LOCUS       DASRWZ010000001         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_342674, whole genome shotgun sequence.
ACCESSION   DASRWZ010000001 DASRWZ010000000
VERSION     DASRWZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342674"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2425
                     /locus_tag="VFH60_00005"
     CDS             <1..>2425
                     /locus_tag="VFH60_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6312189.1"
                     /translation="KQAYRQADQILGDLMGLMGENTNVFVGADHGFAATWKSVGANQV
                     LQDAGLYDAANRGNSKAVAYIAGGTGHIYINLKDREVGGIVEAADYETVRTQIMDAFN
                     NLTDGGAKVVGAVLKKEEARTIGAGEYTVNGWHPTRTGDVLVILQPPYQFDAPTTGQQ
                     IADAPFYGQHGFLPDLVDLDNNINMHSMFGMYGPNVAKGKKIKSPSIVDVVPTAAYAM
                     NLPVPRWSTGRVLTEAFADNSASLVSIQVLAWGDYHGQLDPVTASVDSRSIPSGGVAA
                     IGAYWKEAKAKNPNGTIILSDGDNIGATPPNSAFLNDDPTIEAMNQLGFTASAMGNHE
                     FDKGVAGFQRIHALARFPYLANNIVDAATGRPADFAKPYIVVKANRIDVGIIGIANPE
                     TPNVTSPQGIRGYNWVNPVAPTNNNVRELVAQGVKTIIVVYHQGSISGDFDTVNGIFG
                     DLVQQLDPEVDLVVGGHTRVKTMTRVNGILVSAANHAMETSEMTLLVDPQTKNVAWSW
                     GAFRKPFTPAITPEPGLAALVKDANDKIKPVLGEQVGTAAALVDRSRGAESKMGNLVS
                     DAIRATYQVDVALQNSGGLRADFQPGPVNKGDVFAVLPFGNAVVTGKLKGSDLLAALE
                     NGVSDVSGSAGRFIQLSGVRFAYDASQPVGKRVLWAVLSDGKAVDPNATYNVATNDFM
                     QVGGDGYTALTRMTEVTSREQLWEVAANYVKSLGNVDPQVEGRIIAAQAGQPAPTAPA
                     AATPALPTPASILPTAAPVQSTPAVQPQVTTVAGGPAPTSVAPGMPRTGSTDNTGLLL
                     VLLGISVALL"
BASE COUNT          528 a          767 c          727 g          403 t
ORIGIN      
        1 aagcaggcgt ataggcaggc cgaccagata ctcggtgacc tcatgggctt gatgggcgag
       61 aacaccaacg tctttgtagg cgcggatcac ggcttcgcgg ctacgtggaa gagcgtgggc
      121 gcgaatcagg tgctacagga cgccggactc tacgacgcgg ccaaccgggg taatagcaag
      181 gccgtagcct atatagcggg cggcacaggc catatctaca tcaacctcaa ggaccgcgaa
      241 gttggcggta tagtcgaggc tgccgactac gagaccgttc gcacacagat aatggacgcc
      301 ttcaacaacc tgaccgatgg cggcgctaaa gtggtaggtg ccgtcctcaa aaaggaggaa
      361 gcgcgcacaa taggagcggg cgaatacact gtcaacggct ggcatcccac gcgcacgggt
      421 gacgtgttgg tgatcctcca gccgccttac cagttcgacg cgcccacaac aggccaacag
      481 atcgccgacg cgcccttcta cgggcagcac ggcttcctgc ccgatcttgt ggacctcgac
      541 aacaatatca acatgcactc catgttcggc atgtacggcc cgaatgtggc taagggcaag
      601 aaaatcaagt ccccctccat agtggatgta gtgcctacgg ctgcctatgc tatgaacttg
      661 cccgtgccgc gctggtccac tgggcgcgtg cttaccgagg cgttcgccga caacagcgcg
      721 agcctcgttt ccatccaggt gcttgcctgg ggcgactatc acggccagtt ggaccccgtg
      781 accgccagcg tggacagccg gagcataccg agcggtgggg tagcagccat cggcgcttac
      841 tggaaggaag ccaaagcgaa aaaccccaac ggcacaatca tcttgagcga tggcgacaac
      901 atcggcgcga ccccgcccaa ttcggccttc ctcaacgacg accccactat agaggcgatg
      961 aaccagctag gcttcaccgc atctgctatg ggcaaccacg agttcgacaa gggcgtggcg
     1021 ggcttccagc gaatacatgc cctcgcacgg ttcccctacc tcgccaacaa catcgttgac
     1081 gccgccacag ggaggcctgc cgacttcgct aagccctaca ttgtagtgaa ggctaaccgc
     1141 atagacgttg gtatcatcgg tatagccaac ccggaaacac ctaacgtcac cagcccgcaa
     1201 ggcatacgcg gctataactg ggtaaacccg gtagcgccca ccaacaacaa cgtcagggag
     1261 cttgtagccc agggtgtcaa aacgatcatc gttgtctacc accagggttc gataagcggc
     1321 gacttcgaca ctgtgaacgg catcttcggc gacctcgtcc agcagctcga cccggaagta
     1381 gacctggttg tgggagggca cacccgtgtc aagacgatga cccgcgtgaa cggcatcctg
     1441 gtaagtgcgg ctaaccacgc gatggagacc agcgagatga ccttgctggt ggacccccag
     1501 actaagaacg tcgcctggtc gtggggcgct ttccgcaaac cgttcacccc cgctattaca
     1561 cctgagcctg gactggccgc gctggtgaaa gacgccaacg ataagatcaa gccggtgctc
     1621 ggcgagcagg taggcacggc agccgccctc gtagatagga gtcgtggcgc ggagagcaag
     1681 atgggcaacc tggtgagcga cgctatacgc gctacctacc aggtagatgt cgcactgcaa
     1741 aactccggtg gcctgcgcgc cgatttccag cctggtccgg tcaacaaggg cgacgtgttc
     1801 gccgtgctgc ccttcggcaa cgcggtggta acaggcaagc tgaaaggctc tgacctgctg
     1861 gccgcgcttg agaacggtgt gagcgacgtg tcgggcagcg ccgggcgctt catccagctt
     1921 tcgggcgtgc gcttcgcgta tgacgcctcg cagcccgttg gcaagcgcgt gctgtgggct
     1981 gtgctctccg acggcaaagc ggtagacccc aacgccacct acaacgtagc taccaacgac
     2041 ttcatgcagg tgggcggcga tggctacacc gcgctgacca ggatgaccga agtcacgtcc
     2101 cgcgagcagt tgtgggaggt agcggccaac tacgtcaaga gcctgggtaa cgtggaccca
     2161 caggtggagg gacgcattat cgcggcgcag gcgggacagc ccgcacctac cgcgcccgct
     2221 gccgccaccc cagcgctgcc tactcccgca tctatactgc caacggcggc acccgtgcag
     2281 agcacacctg ctgtccagcc ccaggtaaca acggtggcag gcggtccggc cccaaccagc
     2341 gtagcccccg gtatgccgcg gactggcagc actgacaaca ccggcctgct gttggtgctg
     2401 ctcggcatca gtgtagcctt actgc
//
LOCUS       DASRWZ010000002         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_96,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000002 DASRWZ010000000
VERSION     DASRWZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFH60_00010"
     CDS             <1..208
                     /locus_tag="VFH60_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312190.1"
                     /translation="YYTAVGLADDRHRRFSAAGIALAALALDPFYKTKLKLGLLLRSG
                     LGEGAFGRISHTLHARVPHETRGI"
     gene            254..1462
                     /locus_tag="VFH60_00015"
     CDS             254..1462
                     /locus_tag="VFH60_00015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6312191.1"
                     /translation="MSTILQAEYPHVDEAIDKPGIAASLTPGAARLRVGEVNSRANFM
                     QMQDEWNALVRATGDQPFYRHEWFRIWLDNFAPDARLRVLVARDGEGRLVGALPLIEQ
                     RGMITGAPVRCLSAAANEHTPRFDLVAEDPMEVAAAFLDHLRRDKSWDVLHLASVPPE
                     GHTWRLYEAANRAGNPTGTWDGENSPYFSLPASQEEMQKRLDTKFKANLRRRHKKLAE
                     KGELAVEKVRGGIEMPHRLEEGFAVEQSGWKGREGTAIAQDAHVAGFYSELARFAADD
                     GSLAMFFLRVDGRPVAFQYGLTYGSTYYLLKPGYDEGVKECSPGQLLMDEVVKQCIEG
                     GLSEFDFLGPDMTWKRDWASESHQHSRLYIFRDGGYGRFLRNYKFRWTRQVKHVVSTL
                     LGFAAGFGIG"
     gene            1462..>2253
                     /locus_tag="VFH60_00020"
     CDS             1462..>2253
                     /locus_tag="VFH60_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6312192.1"
                     /translation="MKRTEYFVPALPTLWPGMLLPRMGRRDGRSGFFPFDRPSASYYY
                     FSRNALWEAIKLLGLPGGEVVVPAYHHGVEIETLVAAGVKPVFYPVGRRWDVNPADVE
                     ALIGPNTRALYLEHYAGFPGPTRQMRVLADHYNLPLIEDCALSLLSADGSTPLGSTGD
                     VAFYSIYKMLPVPNGGVMVLNGALAERAAELGTLQVPPFTSTLSHILSSLLQNMEFRG
                     GRAGRAVRKGVRRLGKRTVETAGIERVTTGSDHFNPDDAGLGMSGL"
BASE COUNT          408 a          673 c          791 g          381 t
ORIGIN      
        1 gtattacacc gccgtggggt tggccgacga ccggcacagg cgcttctccg ccgccgggat
       61 agccctggcc gcgctggccc tggacccgtt ctacaagaca aaactcaagc tgggcctgct
      121 tctgcggtcc gggcttggtg agggcgcatt cgggcgcatc agccacaccc tgcacgccag
      181 ggtgccgcac gagacgagag gtatttagcg gcccctgagc cgtcgcaagc gcgagacacc
      241 acccggagca cacatgtcaa ctatcctaca agccgaatat ccccatgtcg atgaggctat
      301 agacaagcct ggaattgccg cgtccctcac cccgggtgcc gccaggctga gggtgggcga
      361 ggtgaatagc cgcgccaatt tcatgcagat gcaggacgag tggaacgcac tggtgcgggc
      421 taccggcgac cagcccttct accggcacga gtggttccgc atctggctgg acaacttcgc
      481 cccggacgcc cggttgaggg tgctggtggc acgcgacggg gagggcaggc tggtgggggc
      541 cttgccgctc atcgagcagc gtggcatgat tacgggtgcg ccggtgcgct gcctgtctgc
      601 cgccgccaac gagcatacgc cccgtttcga cctggtagcc gaggacccta tggaggtagc
      661 agccgccttc ctggaccacc tgagacggga caagagctgg gacgtgctgc acctcgcgag
      721 cgtgccgccg gagggccaca cctggcgctt gtatgaggcg gccaaccggg caggcaaccc
      781 gacgggcacc tgggacggcg aaaactcgcc gtatttcagc ttgcccgcca gccaggagga
      841 gatgcagaag cggcttgaca ccaagttcaa ggccaacctc cggcgcaggc acaagaagct
      901 ggccgagaag ggggaattgg cggttgagaa ggtaaggggc ggaatcgaga tgccgcatcg
      961 cctggaagaa ggcttcgcgg tggagcaaag cggctggaag gggcgggaag gcacggccat
     1021 cgcacaggac gcgcacgtgg cgggcttcta ctcggagctt gcccggttcg cggcggacga
     1081 cggcagcctg gccatgttct tcttgcgggt ggacggcagg cccgtggcct tccaatacgg
     1141 ccttacatac ggctcgacct attacctgct caagcccggc tacgacgagg gcgtgaagga
     1201 gtgcagcccc ggccaactgc tgatggacga ggtggtgaag cagtgcatcg agggcggcct
     1261 gtccgagttc gacttcctgg gaccggatat gacctggaag cgggactggg cgagcgagag
     1321 tcaccagcac tcccggctgt atatcttccg cgacggcggg tacggtaggt tcctgcgcaa
     1381 ctacaagttc cggtggacgc ggcaggtgaa gcacgtcgtg tcaacattgc tggggttcgc
     1441 cgcagggttc gggataggtt agtgaagcgg accgagtatt ttgtcccggc gctgcccacg
     1501 ctgtggccgg gcatgttgct gccgcgcatg ggccgtagag atggccgcag cggcttcttc
     1561 ccatttgata ggccgtccgc gtcgtactac tatttctccc gcaacgcctt atgggaggcg
     1621 atcaagctgc ttgggctacc gggcggcgag gtggtagtcc cggcctacca tcacggcgtg
     1681 gagatcgaga cgctggtggc tgccggggtg aagccggtgt tttacccggt ggggcgcagg
     1741 tgggatgtga accccgccga cgtggaggcg ctaatcgggc cgaacacgag ggcgctgtac
     1801 ctggaacact acgcgggctt ccccggcccc acacgacaga tgcgggtact ggcggaccat
     1861 tacaacttgc ctctcatcga ggactgcgcc ctgtcgctcc tctccgccga cggcagtaca
     1921 ccgctgggca gcacgggcga tgtagccttc tattccatat acaagatgct cccagttcct
     1981 aatggcggag tgatggtgct caatggggca ttagccgagc gtgccgccga gttggggacg
     2041 ctgcaggtac ccccgttcac ctcgacgttg agccatatac tgtcgtcgct gctgcagaat
     2101 atggagtttc gcggcgggcg ggccgggcgg gcggtaagga agggcgtgcg gcggcttggc
     2161 aagcgcaccg tggagacggc cgggatagag cgcgtgacga cggggagcga ccacttcaac
     2221 cctgacgacg cggggctggg catgagcggg ctg
//
LOCUS       DASRWZ010000003         3404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_267,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000003 DASRWZ010000000
VERSION     DASRWZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..650
                     /locus_tag="VFH60_00025"
     CDS             75..650
                     /locus_tag="VFH60_00025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003140489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6312193.1"
                     /translation="MPKPVHILGFAGSLRKQSYNRALLAAAEDYLPEGVTLEQYDIAG
                     IPLFDQDVLDSDGYPEPVQRFRDRIAAADAVLIATPEYNYSMPGVLKNALDWASRPPS
                     PPFNGKPVAVMGASDGPWGTIRAQLHLRQVFVFLNALPVQQPQVMVPKASDKFDDHGR
                     LTDERTADQVRALLEALANWTRRLRGEKPTG"
     gene            complement(832..1911)
                     /locus_tag="VFH60_00030"
     CDS             complement(832..1911)
                     /locus_tag="VFH60_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HET6312194.1"
                     /translation="MSEPVEISAQLLAYISSQRVARLATADAEGKPSLVPVCFAYHAG
                     RFYIALDDKPKRVAPTRLKRVRNILDNPNVALLLDSYYEDWTRLAYVLVSGTARLEEQ
                     GSAAHVSAIGVLREKYPQYREMAIDRQPVIAITPASIRAWKGSQSEPQDDVPADRREL
                     DFASLARGRHVVRRFLTREVPASLVEQTLEAARWAPSPHGMQPWRFVVVTRPELKRKL
                     ADAMSVEWRHNLEMDGESPEVVEDRLRKSRNRLLNTPVLVIPCLYMVGLHEYPDPARA
                     AAEEIMAIQSLGAAVQNMLLSAYSLGLDTGWMCAPLFCPGVVREVLDLDPDLSPHALI
                     QLGYAAIDPPRRPHRPISDLIVRYD"
     gene            complement(1889..2905)
                     /locus_tag="VFH60_00035"
     CDS             complement(1889..2905)
                     /locus_tag="VFH60_00035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6312195.1"
                     /translation="MSQNYIGYAVMGEQFHPTDLVNYSKAAEDNGFTALMVSDHFHPW
                     VPSQGHSPFAWALLGAIGEATNLRFGTGVTPPGWRYHPAILAQAAATLEAMYPGRFYL
                     GLGAGEALNEHIVGQYWPEAVTRMETLRETIEVIRNQFSGKEVKHRGSYFNLESARLY
                     TMPDNPPPIYVATSGPVNAERTGRTCDGLITVGAADEKIRMLLGRFEKGAREAGKDPD
                     EMPRIVQLHVSWAETDEQAVEQAVKEWPNGGMNFPKADIRNPEDFEAMTKLVRPENFK
                     GRVLMSADLEAHLGHLQHYADLGFNELYIHNVGRNQYEFMQAYGREVIPNVKWGRFER
                     AGRD"
     gene            3208..>3404
                     /locus_tag="VFH60_00040"
     CDS             3208..>3404
                     /locus_tag="VFH60_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312196.1"
                     /translation="MSDSTAANNIPQAPTADVQDAASPAPRNAWWLTTRRRRAEWQSL
                     MATGLATFCGRPRAHFESANRG"
BASE COUNT          600 a         1101 c         1048 g          655 t
ORIGIN      
        1 tatctgtctt acactccctt tatgcctttc cgaaggcagt tggtgtaaag aacgagacaa
       61 aaggagaata ttagatgcct aagcctgttc acatactcgg ctttgcgggg agtttacgca
      121 aacaatccta caaccgtgcc ctccttgcgg ccgccgagga ctacctgccg gagggcgtaa
      181 cgctcgaaca gtacgacatc gcgggcatac cgctgttcga ccaggacgtg ctcgacagcg
      241 acggctaccc agagccggtg caacgcttcc gcgacaggat agcggccgcc gacgccgtgc
      301 tgatagccac cccggagtac aactactcga tgcccggcgt gctcaagaac gcgctagact
      361 gggcctcccg cccgcccagc ccgcccttca acggcaagcc tgtagccgtc atgggcgcga
      421 gcgacggccc gtggggcacc atacgcgccc agctacacct gcgacaggtg ttcgtcttcc
      481 tgaacgccct ccccgttcag cagccgcagg tgatggtgcc caaagctagc gataagttcg
      541 acgaccacgg ccgcctcacc gacgaacgca ccgccgacca ggtacgcgcc ctcctcgaag
      601 ccctcgccaa ctggacccgc cgcctccggg gtgaaaagcc aacagggtaa gagacgaaaa
      661 cgcaaagacg caaagaacgc aaagaacgcg cagagataaa attaaaggtt ttctaagcgg
      721 aaataggacc agggtacaac atgacgtact ctggtcctct ttgtactatc tgaaccattc
      781 ttctctttgc gcgttctttg cgttctttgc gtctttgcgt tttcgtctcc ttcaatcgta
      841 ccgcacgatg aggtcggaga tgggacggtg ggggcggcgg ggtgggtcta tggcggcgta
      901 gcctagctgg atgagggcgt gtgggctgag gtcggggtcg aggtcgagca cctcgcggac
      961 gacgccgggg cagaagaggg gggcgcacat ccagcccgtg tcgaggccca ggctgtaggc
     1021 gcttaggagc atgttttgca ccgccgcgcc caggctctgg atagccatta tctcctcggc
     1081 ggcggccctg gcggggtcgg ggtattcgtg caggcccacc atgtaaaggc aggggatgac
     1141 cagcacaggg gtgttgagca ggcggttgcg cgacttgcgc aggcggtcct ccaccacctc
     1201 ggggctttcg ccgtccatct ccaggttgtg ccgccactcc accgacatag catcggccag
     1261 cttccgcttc aactccggcc gggtgacgac cacgaagcgc cagggctgca taccgtgagg
     1321 ggagggcgcc catctcgccg cctcaagcgt ctgctccacc agcgacgcgg gtacctccct
     1381 tgtcaaaaac cgccgcacca cgtgcctccc ccgcgccagt gaagcgaaat caagctcccg
     1441 gcggtcggcg ggcacgtcat cttgcggctc ggattggcta cccttccacg cccgtatcga
     1501 agcaggcgtg atagcgatta ccggctgcct gtcgatagcc atctcgcggt actgggggta
     1561 cttctcgcgc aacaccccta tagccgagac gtgggctgcg ctgccctgct cttccaggcg
     1621 ggcggtgccg ctcaccagca cgtaggccag ccgggtccag tcctcgtagt agctgtccag
     1681 cagcagggcc acgttggggt tatccaggat gttgcgcacc cgcttgagcc gcgtaggggc
     1741 cacgcgcttg ggcttgtcat ccagggcgat gtagaaccgg cctgcgtgat acgcaaagca
     1801 caccggcacc agcgagggtt tcccctctgc gtccgcggtg gcaaggcggg cgacccgctg
     1861 cgagctgatg taggcgagca gttgcgcgct aatctctacc ggctcgctca aatctgcccc
     1921 actttacgtt gggtatcacc tcgcgcccgt aagcctgcat gaactcgtac tggttgcggc
     1981 ccacgttgtg tatatatagc tcgttgaagc cgagatcggc gtagtgctgc aagtggccga
     2041 ggtgcgcctc caggtcggcg gacatgagca cgcgtccctt gaagttctcc ggcctcacca
     2101 gcttggtcat cgcctcgaag tcctccgggt tgcggatatc ggccttgggg aagttcatgc
     2161 cgccgttggg ccactccttg accgcctgct ccaccgcctg ctcgtccgtc tcggcccagg
     2221 agacgtgtag ctggacgatg cggggcatct cgtcggggtc tttgcccgcc tcccgcgctc
     2281 ccttctcgaa gcggcccagg agcattcgaa tcttctcgtc ggcggcccct accgtgatga
     2341 ggccgtcgca ggtgcgcccg gtgcgctcgg cgttcacggg gccggacgtc gccacgtaga
     2401 tgggcggggg gttgtcgggc atggtgtaga ggcgggcgct ctccaggttg aagtagctgc
     2461 cccggtgctt gacctccttg ccgctgaact ggttgcgtat cacctctatc gtctcgcgca
     2521 gcgtctccat ccgggtcacc gcctccggcc agtactgccc gacgatgtgc tcgttgaggg
     2581 cctcgcccgc gcccagcccc aggtagaagc ggcccgggta catcgcttcc agcgtggcgg
     2641 cggcctgcgc cagtatggcc gggtggtagc gccagccggg cggggttact cccgtgccga
     2701 agcggagatt ggtggcctcg ccaatggccc ccagcaaggc ccacgcgaag ggagagtgcc
     2761 cctgcgaagg cacccagggg tggaagtggt cggacaccat gagggccgtg aagccgttgt
     2821 cctctgctgc tttgctgtag tttaccaggt ccgtcgggtg aaactgctcg cccatgacgg
     2881 cgtaacctat gtagttctgg ctcatgattg ctccggattt ggaaattggg attgcggatt
     2941 ttaggtattt gtcctgctat tataaatctt gttacatgtt ccgtgccgag gtcgccctgc
     3001 catcatcgcc ctgtggctga agccacgggc ttacaatggg gaaagtcccc tgcggggact
     3061 gaggaacatc atgtagtctc cgaaggagac ttcgtccctg gtcagcccgc cactttagtg
     3121 gcagggcgtc ccctgccgtt tcattgccga gaatgatctc ctaaactatt gcagctataa
     3181 gtccgtgggt gtatataata tgtcaacatg agcgactcaa ccgccgccaa caacataccc
     3241 caagccccca cagccgacgt ccaagacgcc gcctcccccg ccccgcgcaa cgcctggtgg
     3301 ctcaccacgc gccgccgccg cgccgagtgg cagtcgctca tggcgaccgg cctggctacc
     3361 ttctgtggcc gcccacgcgc ccacttcgag tcggccaaca gggg
//
LOCUS       DASRWZ010000004         1718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_548435, whole genome shotgun sequence.
ACCESSION   DASRWZ010000004 DASRWZ010000000
VERSION     DASRWZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548435"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFH60_00045"
     CDS             complement(<1..567)
                     /locus_tag="VFH60_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312197.1"
                     /translation="MIADRDSRAIVRSLFACVLLLLLAGLSGGLLAGGASYAGASRAG
                     ASPTATAAAPTISAGGTATPTPCTNTTQLLFEGFESGTLGLFTSTVIISSTFTPVPTP
                     GWAASTATPRTGSYAAFAPGPDRITDQRLELSDVIVIPTGVSSATLTFWHNFDLEQSF
                     DGGVLEVSTDSGITWADADRNIIVGGYNS"
     gene            complement(645..>1718)
                     /gene="fabF"
                     /locus_tag="VFH60_00050"
     CDS             complement(645..>1718)
                     /gene="fabF"
                     /locus_tag="VFH60_00050"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET6312198.1"
                     /translation="NPEDYIPAKEIKRMDRFIHYAIAVCKQAAAQGDLQITPENATDI
                     GVVIGSGIGGIETLTESVMIIHEKGPDKVSPFAIPMLLTDLAAGQVSIQMGMKGPNFA
                     VVSACSTSAQAIGEASEIIRRGQAKAMFAGGAEATVCKVGLATFTAMRAISRRNDEPE
                     KASRPFDKLRDGFVLGEGAAALLLEDLEFAQARGANILAEITGYGASADASHVTAPAE
                     GGEGAARAMTMALRNAGLEPKDIDYINAHGTGTPLNEKYETMAIKTAFGDHAYKLAVS
                     STKSMTGHMMGATGAMELIFCIKAIREGIIPPTINQEVPDPDCDLDNVPNVARVRDVR
                     RAMSNSMGFGGHNAVLIVERFDG"
BASE COUNT          304 a          548 c          520 g          346 t
ORIGIN      
        1 ggaattgtat ccgccgacta tgatattccg gtcggcatca gcccaggtaa taccactatc
       61 tgtggatact tccagcaccc ccccatcgaa gctttgctcc aggtcgaaat tatgccagaa
      121 ggtgagggta gcgctgctca cgcctgtggg tataactatc acgtcggaaa gctccaggcg
      181 ctggtcggtt atcctgtccg ggccgggcgc gaaagccgcg taggagccgg tgcgtggggt
      241 agcggttgag gcagcccacc caggggtagg cacaggagtg aaagtagaag atatgataac
      301 ggtggaggta aagaggccca gcgtgccgct ctcgaaaccc tcaaagagca gttgggtggt
      361 attggtgcaa ggcgtagggg tagccgtccc gccggccgag atcgtagggg cggcggcggt
      421 ggcggtaggc gatgcccccg cgcgagaggc acccgcatag gacgcgcccc cggccaacag
      481 cccgccggac aggcccgcca ggagcagaag cagcacgcac gcgaataacg agcgtactat
      541 agctcgacta tctctgtctg ctatcatgtg cccctcctct ccgggaagcg cgtattgcga
      601 ggcccaggct gccgctagtg tcccctgggc ctctttacct gtgcttaccc gtcgaatcgc
      661 tccacgatga gcacggcatt atgcccgccg aagcccatac tgttcgacat ggcgcgccgg
      721 acgtctctca cgcgcgccac gttcggcacg ttatccaggt cgcagtcggg gtcgggtact
      781 tcctggttga tggtgggcgg gatgatgccc tccctgatcg cttttatgca gaagataagc
      841 tccatagcgc ccgtcgcgcc catcatgtgc cccgtcatgc tcttggtgga cgacacggcc
      901 agcttgtacg catggtcccc gaatgctgtc ttaatagcca tcgtttcgta cttctcgttc
      961 agaggggtgc ccgtgccgtg cgcgtttatg tagtcaatat ctttaggctc aagcccagcg
     1021 ttacgcaggg ccatcgtcat ggcccgcgcc gcgccctccc caccttctgc gggggctgtg
     1081 acgtgcgacg catcggctga cgcgccgtag cccgtgatct ccgccaggat attcgcgccc
     1141 ctggcctggg cgaactcaag gtcttccagc agaagtgcag ccgcgccctc gcccagcacg
     1201 aagccgtcac gcagcttgtc gaacgggcgc gaggctttct caggctcgtc gttgcgcctg
     1261 gagatagcgc gcatcgccgt gaaggtcgcc aaccccacct tgcacacggt agcctcggcc
     1321 ccgcctgcga acatggcctt cgcctgcccg cgcctgatga tttcgctggc ctcgccaatc
     1381 gcctgcgccg aggtcgagca ggccgagacc acagcgaagt tcggcccctt catccccatt
     1441 tgtatagata cctgccccgc cgccaggtcg gtcagcaaca tcggtatggc gaaagggctg
     1501 accttgtccg gccctttctc gtggatgatc atcaccgact cggtcagcgt ctcgatgccg
     1561 ccgatgccgg agcctatcac cacgcctatg tcggtagcgt tctccggggt tatctggaga
     1621 tcgccctggg cggcagcctg cttgcacacc gcaatggcgt aatggatgaa gcggtccatg
     1681 cgctttatct ctttggcagg tatgtaatcc tctggatt
//
LOCUS       DASRWZ010000005         1885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_137444, whole genome shotgun sequence.
ACCESSION   DASRWZ010000005 DASRWZ010000000
VERSION     DASRWZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137444"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VFH60_00055"
     CDS             complement(<1..157)
                     /locus_tag="VFH60_00055"
                     /inference="COORDINATES: protein motif:HMM:NF044561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET6312199.1"
                     /translation="MAVQYVLSDYVDHAMSEATYDKLEDGTYAGSIPSCPGVMSFGET
                     LRECQDEL"
     gene            complement(170..1045)
                     /locus_tag="VFH60_00060"
     CDS             complement(170..1045)
                     /locus_tag="VFH60_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6312200.1"
                     /translation="MVQTLERPDTEERAPDSDNIVQGKISLVLPAHNEEPNIRAVVEE
                     AAEVLPTAFSDYEVIVVNDGSKDRTLEIAETLAQENPHVRVVNHPVNRGYGAALTSGF
                     NAATGDYVMFMDSDRQFDINDIHRLTPHVPDYDIVAGYRIKRNDPAHRLLNAAIFGMA
                     VRLMFDIRVRDIDCAFKVMHADVLRGINLESPGALINTEILAKAKVQGCTLTQVGVYH
                     YPRQAGEQSGANLKVVLRAFREIIRLWWRMRTYSPPSGEVVQPPPTWRGFALFGSAAA
                     MGLLFLRGLLRLFKK"
     gene            complement(1286..1720)
                     /locus_tag="VFH60_00065"
     CDS             complement(1286..1720)
                     /locus_tag="VFH60_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET6312201.1"
                     /translation="MNDENGNNHSDHRRTVGGRGEDVAALYLRRRGCTILARRWYANP
                     GEIDIIASCPSRPMGTDALSLERAPTLAFVEVRTRHGHEGMAEESISQRKASSMVTAA
                     YAYMEAHNIDPEATAWRIDLVGVTMLGGKVTDINWVKGAIEE"
BASE COUNT          343 a          577 c          581 g          384 t
ORIGIN      
        1 gtagttcatc ctggcattca cgcaatgtct ctccaaagga cataacccct ggacacgacg
       61 gaatgctgcc cgcgtatgtc ccatcttcta gcttgtcata cgtggcctcg ctcattgcat
      121 ggtccacgta atcgctgaga acatattgca cagccatcag cgtacaacct tacttcttga
      181 acaaccggag caacccgcgc aggaaaagca gccccatcgc ggccgcgctg ccgaacaggg
      241 cgaagcccct ccaggtgggc ggcggctgca ccacctcgcc gctgggcggg gaataggtgc
      301 gcatgcgcca ccacaaccgg atgatctcgc ggaaggcccg cagcaccacc ttcaggttcg
      361 cccccgactg ctcgcccgcc tgtcgggggt agtggtacac gccgacctgg gtgagcgtgc
      421 agccctgcac cttggccttg gccagtatct cggtgttgat gagcgcgccc ggcgactcca
      481 ggttgatgcc cctgagcacg tcggcgtgca tcaccttgaa ggcgcagtcg atgtcgcgca
      541 cgcgaatgtc gaacatcagg cgcacggcca tgccgaaaat agcggcgttg aggaggcggt
      601 gggccgggtc gttgcgcttg atgcggtagc ccgcgacgat atcgtagtcg ggcacgtggg
      661 gggtgaggcg gtggatgtcg ttgatgtcga actgccggtc cgagtccatg aacatgacgt
      721 aatcgccggt ggcggcgttg aagccgctgg tgagggcggc gccgtagccc cggttcacgg
      781 ggtggttcac cacgcgaacg tgcgggttct cctgggcgag cgtttccgcg atttccagcg
      841 tgcggtcctt cgagccgtcg ttgacgacta tgacttcgta gtcggagaaa gcggtaggga
      901 gcacttcggc ggcctcttca acgaccgcgc gtatattcgg ctcctcgtta tgagcgggca
      961 gcaccaggga aatcttgccc tgcacgatgt tatcactatc gggagcgcgc tcttcggtgt
     1021 cggggcgctc aagggtctgg accaacggtt cctccaggct atcgcatcgt cgcgtattgg
     1081 cgcaggcata ccggcatacc ggcgtatgcg ccacaattag cgggcggtgt gtattccact
     1141 tccgcgcagc ctacaatatc accgcacaaa gtatgcccac actaccagcc taagcagttt
     1201 atagcacttg ccgggacgcg tcaagcctgg cgctcggaat tattgcgggg ttggcagggc
     1261 gtccccactg ggctatagca cggcgtcact cctctatagc ccctttcacc cagttgatat
     1321 ccgtcacctt accgcccagc atcgtgacac ccaccaggtc gatgcgccat gccgtggctt
     1381 cggggtcgat gttgtgggcc tccatataag cgtaggccgc cgtgaccatg ctgctcgcct
     1441 tgcgctgcga aatcgactcc tcggccatac cctcgtgccc gtgccgggta cgtacctcga
     1501 caaatgccag cgtcggcgcg cgttcgaggg agagggcgtc ggtgcccatc ggcctggagg
     1561 ggcagcttgc gattatgtca atctcaccgg ggttggcgta ccagcggcgg gccaggatgg
     1621 tgcagccgcg gcgtcgcagg tagagggccg ctacgtcctc tcccctgccg cctaccgtcc
     1681 tgcggtggtc gctatgattg ttgccattct catcgttcat aaattcggag atccacgatg
     1741 tgcagtaggt ccaccaagcg taaataatct acaaaccgac cactttgcac agcgtcaggc
     1801 cagaaaagtc tcgtaacatt gcgcgtggtt tggcagttgg cgcggatgta tagctaggag
     1861 cctactttct ggcagggttt ggcaa
//
LOCUS       DASRWZ010000006         3749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1233938, whole genome shotgun sequence.
ACCESSION   DASRWZ010000006 DASRWZ010000000
VERSION     DASRWZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233938"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..750
                     /locus_tag="VFH60_00070"
     CDS             214..750
                     /locus_tag="VFH60_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312202.1"
                     /translation="MFIQRKAVRVALAALLGVMSLLALSSGAAVAGGKPTIFNLEFSL
                     DTPLPAGPGNCAFDVVQHTEGNLKVRVFRDDEGNRVRSIQSFPEYGGYYLNTENGKSV
                     PYEAHGPVLRTFNADGTVSDTMPGVVKANIPGQGVVFINAGSLTFTYFLSDPNNGTIE
                     ITGGQFDDTTADICPYLQ"
     gene            complement(966..2381)
                     /locus_tag="VFH60_00075"
     CDS             complement(966..2381)
                     /locus_tag="VFH60_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312203.1"
                     /translation="MSNEPGGPRRLLILSSQSTNGDLDGPPDLKRARGGALGVARALQ
                     KEARWPEGLDLLLLSDAYGLVEPTLPDEEPVPVPFTRAENPNWWDGYVGRNLDNLVQR
                     RGYSDVFVLVRPEHAQAVRASQRLRRLAATWAGSEGSAALDEMKAWIGSLDVPGPGET
                     ALDMATGNLEEHDTSDLRTSQLGQIIPFREPAPPEPEPEPEREPRVSIPAAVHDLIDE
                     AIYSSRFMLVASKLSKEQLGQARRDLDAAWARRTARRHERPSVSNVVVKSARLPWSHA
                     PAVTLYGRLLESVGMQSVLGSINKAVSQAAITEPGRYKEILARLPQDESDFVADLLYL
                     LWEASSRMDKDEIAMLRAYLSDNATHIELRRMGIPRNLTIEDRYEVLRVVLECFSGLA
                     PQAKLADHRRVWVWLDEIENILGYNERERWELVKALETLMGNTPPYLTVWLNISPNSP
                     ATSQEIQAALENNLVITADLT"
     gene            complement(2780..3481)
                     /locus_tag="VFH60_00080"
     CDS             complement(2780..3481)
                     /locus_tag="VFH60_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312204.1"
                     /translation="MPVFVERGWRAFCLRVQLEGPGEVRLLLKEDGGSAREADAARLW
                     PRSCEAGLAVAASGNPVARRVDPFVGAGVLSRDDVTLGGLIRKPSERDPDFVPLAEGA
                     PGVPRLRWEGEVYAEGGRYGLELHTDGQAALSMDGATVLTACSKPLTVQSFFVRGGYP
                     WARAEMDLTPGWHAVGLDFAATGTVNGLEWRWTRPDGVVEIVPPDRLRHKVSFDAPPP
                     TPVAPPDNIDCPPSQ"
BASE COUNT          662 a         1266 c         1149 g          672 t
ORIGIN      
        1 ctcagcaccc aaataacacc tttcctcatg tcccaacccg ctcgaacgca tagccgctct
       61 tgctcccacg aaaactactc gaataagtaa tgtgccgcgc gcccttttgc ctgtagagta
      121 agcgaagagc gcgcacgtat gcagcagagg tacgcataac tgcctatgtg cgcgcttgag
      181 ccttcatgca agcacagtaa aggagaggac ccaatgttca ttcaacgtaa ggcggtgcgc
      241 gtggcgctgg cggcgctcct gggggtgatg tcgctgctgg ccctatcgag cggggcggcc
      301 gtggcgggcg gcaaacccac tatcttcaac ctcgaattca gcctggacac gccactcccc
      361 gccggaccgg gcaactgcgc cttcgacgtc gtgcaacaca ccgaaggcaa cctgaaagtg
      421 cgcgtcttcc gcgacgatga gggcaaccgc gtgcgctcga tccagtcgtt cccggagtat
      481 ggtggctact acctcaacac ggagaacggc aagagcgtgc cttatgaggc gcacggcccc
      541 gttctgcgca ccttcaacgc cgacggcacc gtcagcgata ccatgccagg cgtggtaaag
      601 gcaaacatcc ccggacaggg tgtggtcttc atcaacgcgg gaagtctgac cttcacctac
      661 ttcctcagcg accccaacaa cggcacaatc gagataaccg gcggccagtt cgacgatacg
      721 accgccgaca tctgccccta cctgcaatag tcgccagcct ttctaaacgc caagacgcca
      781 agagtacgaa aacgcaaaga cgcatagaac gcgcaaagag gatgagcgtc taacgggtag
      841 gaaaaggacc agggtgcgtt atatgtaccc tggtcctttc gcttagaaca cctttatctt
      901 aatcttggcg cgctcttggc gtccttggcg tcttggcggt tccgtctcct atcatctacc
      961 accccttacg tgaggtcggc ggtgatgacc aggttgttct ccagggcggc ttgtatctcc
     1021 tgagaggtcg cggggctgtt gggactgatg ttgagccaga cggtgaggta gggcggggtg
     1081 ttgcccatga gggtttcgag cgccttgact agctcccaac gctcgcgctc gttgtagccc
     1141 aggatgtttt ctatctcatc gagccacacc cacacccggc gatggtcagc cagcttggct
     1201 tgtggggcta ggccagagaa gcattccagc actactcgca agacctcgta gcggtcctca
     1261 atcgtcaggt tgcgggggat acccatgcgg cgaagctcga tgtgggtggc gttgtccgac
     1321 aggtaggctc gcagcatggc tatctcgtcc ttgtccatac gcgagcttgc ttcccacagc
     1381 aggtagagca ggtcggccac gaagtcgctt tcgtcctggg gcagccgggc cagtatctcc
     1441 ttgtagcggc ccggctcggt aatggcggcc tggctcaccg ccttgttgat gctccccagc
     1501 acgctctgca tgcctacgct ctccaacagc ctaccgtaca gcgtgacggc gggggcgtgc
     1561 gaccagggca ggcgggcgct cttcaccacc acgttgctca ccgatggtct ctcgtgccgg
     1621 cgggccgtgc gccgcgccca ggccgcatcg aggtcgcgcc gcgcctgccc cagctgctct
     1681 ttgctcagct tgctcgccac cagcatgaag cgacttgagt agatggcctc atcgataagg
     1741 tcgtggaccg cagcgggtat gctcacgcgc ggctcccgtt cgggttccgg ctcaggttcc
     1801 ggtggggcag gctcgcggaa aggtatgatc tggccgagtt gcgacgtgcg caggtcggag
     1861 gtatcgtgct cctcaaggtt gccggtagcc atgtccagcg cggtctctcc cggccccggc
     1921 acgtcgaggc tgcctatcca cgccttcatc tcgtccaggg ccgcgctgcc ctcgctccca
     1981 gcccaggtgg cagccagcct gcgcagccgt tgggaagcgc gcaccgcctg ggcgtgctcc
     2041 ggcctgacca gcacgaacac atccgagtag ccccggcgct gcaccagatt gtccaggttg
     2101 cggcctacat acccgtccca ccagttaggg ttctcggccc gcgtgaaggg caccggcaca
     2161 ggctcctcgt cggggagggt cggctccaca agaccgtaag cgtccgagag cagcagtagg
     2221 tccagcccct ccggccatcg ggcctccttt tgcagcgccc gcgccacgcc caaggcccca
     2281 cctctggccc gcttgaggtc gggcggtccg tccaggtcgc cgttggtgct ctgagaggac
     2341 aatatcagca gcctgcgcgg gccgccgggt tcgttgctca caggttctct cccccttcgc
     2401 cggttatctg tctcgcggac aagatatatt agcagacatt cgagggggat gctatgtgcg
     2461 cgagtgacga gtgacgagtg acgagtgacg agtgacgagt gatgaggact gagtaaggag
     2521 acggaaacgc aaagacgcta aggacgcaaa gggcgcgcaa agtttaggat ggttgggctg
     2581 ccctcctacc ggaatgcagg accagagtcc cccctgtcat cctgagcgca gcgaaggatc
     2641 agccgcccgc tcttgattct ccctattacg gtgacaatgt tccattaccc agcatcatcc
     2701 ccaatgttgt agaccctcgc ctacccacct gatccttcgc tacgctcagg atgatagggc
     2761 cacttgtggc cctgcccttc tactgcgaag gcgggcaatc aatgttgtct ggaggagcta
     2821 ccggggttgg gggtggtgcg tcgaagctta ccttgtggcg caggcggtcg ggcgggacga
     2881 tctcgacgac gccgtcgggg cgggtccagc gccattcgag accgttgacg gtgccggtcg
     2941 cagcgaaatc gaggccgacc gcgtgccagc cgggcgtaag gtccatctcc gcgcgcgccc
     3001 acgggtagcc gccccgcacg aagaaggact gcaccgtcag cggcttgctg cacgcggtca
     3061 gcacggtagc gccgtccatg gagagcgcgg cctggccgtc ggtgtgtaac tccagcccgt
     3121 accgtccgcc ctcggcgtat acctccccct cccagcgcag gcgcggcact cctggtgcac
     3181 cctcggcaag gggcacgaag tcggggtcgc gctcggaagg cttgcgaatg aggccgccca
     3241 gggtgacatc gtcccgcgac agcacgcccg ctcccacgaa cgggtccact ctgcgcgcca
     3301 ccgggttacc gctcgccgcc acggccagcc cggcctcgca cgaccggggc cacaggcgag
     3361 cagcgtctgc ctctctcgcc gagccgccgt cctccttcag caacaggcgc acctcacctg
     3421 gcccctccag ctgcacccgc aggcagaatg cccgccagcc gcgctccacg aacacaggca
     3481 cccctaaccc gccgggctgt ccttcgagca gcgccggccc tcgtgcgctg cctcccaggc
     3541 tgtgtgtgat agggccgggc tgcgcttcgt actacgcccc gctccaggtg accgtgaccg
     3601 ggtagcgcac gtccagcccc ggcggcaagg ctcccacggc gggcacctgg ccccggtgca
     3661 ccaccggccc gcccgcggcc tctatcgtta cctcgacgcc ctgcctgccc tgagactgac
     3721 cggcgggcac gagataggcg cgaccggcc
//
LOCUS       DASRWZ010000007         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_274676, whole genome shotgun sequence.
ACCESSION   DASRWZ010000007 DASRWZ010000000
VERSION     DASRWZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274676"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /gene="dnaG"
                     /locus_tag="VFH60_00085"
     CDS             complement(<1..1391)
                     /gene="dnaG"
                     /locus_tag="VFH60_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET6312205.1"
                     /translation="MDNDVIAEIKSRIDIEDVVGEKVRLSRAGRNLKGLCPFHGEKTP
                     SFVVYPKDGSFHCFGCGQNGDIFGFLMKTEGLDFRGALEKLARKAGVELKPRNEQAAQ
                     EDRQRERLKEACAAAALFYHNLLRQHESAAQARAYAEKRGLTTSTWELFQVGYAPESW
                     DILLNHLTSKGYTVQECVDAGLAAEREEGGAYDRFRNRLLFPIRDHKGAVVGFGGRAL
                     GDNQQPKYLNSPQSAVFDKSQILYGLDLARPNIQRSGQVVIVEGYMDVVVAHQAGQTN
                     VVGTIGTALTDGHAELLKRIARKVILCLDPDTAGDMAALKGSEVLQEHADKIAIPVRG
                     ERGLMGVERRSQLEIRVMQLPRGMDPDELLLAEGGPARWDELREGALPLVDHVIGVVA
                     AKHDLNSAHGKSEAVGEMALYVREIGDPVQRAHYMQRVASVLRVPEDAVREAISRATP
                     QKRDGRKARERDEG"
     gene            1958..>2095
                     /locus_tag="VFH60_00090"
     CDS             1958..>2095
                     /locus_tag="VFH60_00090"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HET6312206.1"
                     /translation="MVTFPLREQISYPGGGASVQRQPVDSTNLRSVGYDEEQQILEIE
                     FI"
BASE COUNT          368 a          674 c          634 g          419 t
ORIGIN      
        1 ccttcgtccc gctccctcgc cttgcgcccg tcgcgcttct gcggggtggc cctgcttatc
       61 gcctcgcgca ccgcgtcttc gggcacccgc agcaccgagg ccacacgctg catatagtgg
      121 gcgcgctgca ccgggtcgcc aatctcgcgc acgtagaggg ccatctcgcc tacagcctcg
      181 ctcttgccgt gcgcgctgtt caggtcgtgc ttggccgcca ccacgccgat tacgtggtcc
      241 accagcggca aagccccctc ccgcaactcg tcccaccgcg ccgggccgcc ctcggccagc
      301 agcaactcgt cggggtccat gcccctcggc agctgcatca cgcggatctc aagctgcgac
      361 cgtcgctcca cgcccatcag cccgcgctcg ccccgcaccg ggatggcgat cttgtccgcg
      421 tgctcctgta gcacctcgga gcctttaagg gccgccatgt cgcccgccgt gtcggggtcg
      481 aggcagagga tcaccttgcg ggcgatacgc ttgagcagct cggcatgccc gtcggtgagg
      541 gccgtgccga ttgtgcccac cacgttggtc tgccccgcct ggtgcgccac caccacgtcc
      601 atgtacccct cgactatcac cacctgcccg ctacgctgta tgttgggccg ggccaggtcg
      661 aggccgtaca gtatctggct cttgtcgaac accgccgact gcggcgagtt gaggtacttc
      721 ggctgctggt tgtcgcccaa cgcccgcccg ccgaagccca ccaccgcgcc tttgtggtcg
      781 cgtatgggga agagcaggcg gttgcggaag cggtcgtacg ccccgccttc ctcccgctct
      841 gccgccaggc ccgcgtccac gcactcctgc accgtgtacc ccttcgatgt aaggtggttc
      901 agcaggatgt cccacgactc cggcgcgtac ccgacctgga acaactccca ggtggaggtg
      961 gtgaggccgc gcttctcggc gtaagcacgt gcctgggctg ccgattcgtg ctggcgcagc
     1021 aggttgtggt agaagagggc ggcggcggcg caggcttcct tgaggcgttc gcgctggcgg
     1081 tcctcttggg ctgcctgctc gttgcgcggc ttgagctcga ccccggcctt gcgagccagc
     1141 ttctccaacg cgcccctgaa atcgaggccc tcggtcttca tcaggaagcc gaatatgtcg
     1201 ccgttctgcc cgcagccgaa gcagtggaag gagccgtcct tggggtacac cacgaaggaa
     1261 ggggtcttct cgccgtggaa cgggcagagg cccttcaggt tgcggcccgc cctgctgagg
     1321 cgcaccttct ccccaacgac atcctcgatg tcgatgcggg acttgatctc ggctataacg
     1381 tcattatcca tgcagcgtca tatagtgggt gccgggtgcg acgtagggct acaagctggt
     1441 cgggtaggcc gaggagtggt aaggggtgca tcacggacgc ttcgagcggg tagcaggcga
     1501 gcacagtgat tacacgctag tataactccg taatgtggaa atctgagtcg gcaaaagtgc
     1561 caagttttcc acatagcagc tacttgctgg aagaatttac ccaaaatgtg gcacttaagc
     1621 tgccaatctt gccccacatt tagtataacg atgttgtgga tgcgctttgg gaccatcgcc
     1681 caccgcgacg catcctgctc ttgcggatgg agttgcagcc tgtatccggg tgcagattcg
     1741 tgggaatagt ggatgcattg catccacgta gcgggtctat tcctatggcg tcctgtagcg
     1801 ccaatcaagg cggggatgca gtcgaatagc aagaccaaag ctccacagcc aagaagggga
     1861 ggcggattcg tcacttcgtt cagaatgaca ggggaagatt cataccgtac aagcagcgcc
     1921 ttgcaaaata ctcctggtcc ataccatacg ttgtataatg gtaacgtttc cgttgcggga
     1981 acagattagc tatccaggag gtggcgcttc cgtgcaaagg cagcctgtag actccactaa
     2041 cttacgctcc gtcggctacg acgaggagca gcagatattg gagatagagt tcatt
//
LOCUS       DASRWZ010000008         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1165471, whole genome shotgun sequence.
ACCESSION   DASRWZ010000008 DASRWZ010000000
VERSION     DASRWZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165471"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1371
                     /gene="solA"
                     /locus_tag="VFH60_00095"
     CDS             238..1371
                     /gene="solA"
                     /locus_tag="VFH60_00095"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HET6312207.1"
                     /translation="MYDLIVIGLGAMGSATAYHAASRGHRVLGLDAYERNHANGSSHG
                     RSRIIRESYAEGPEYVPLVQRAYKLWRDLEEESSRSLLTIIGGVYIGSPGTEIVRGVL
                     RSAEEHGLACDYLSAAELMSRFPGFRVPEDMVAVYEANGGVLDAEACVGAHLDLAARH
                     GAELRHGEPVLRWAADGGGVRVETAAGSYTGDRLVIAAGPWIGGLLADLSLPLAVWRV
                     YNTFFEPTRPEFEVGRCPFYLLEVPEGTYYGLPSLPGQGLKAGRHDAGEVSTPQNVRR
                     TVDGSEVEAIRSVLERYMPGAAGALKATTTCVYTVTPDADFVIDRHPEHPQVVYASPC
                     SGHGFKFSSAIGEVLCDLALEGTSAFDVAVFSAERFGIGAGRK"
     gene            1586..2317
                     /locus_tag="VFH60_00100"
     CDS             1586..2317
                     /locus_tag="VFH60_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HET6312208.1"
                     /translation="MFFFDTRYIFFVMIPGIVLMLLASWWVKNAFEKGSKIPNLQRLT
                     GAQVARMILDSSGLGHVAVQPTQPHGLSLSSGASPDLDDHYDPREKVLRLSPRVYGSA
                     SVAAIAVAAHEAGHAIQDAQGYALMRARTAIVPIANIGSQLGGFIVILGVIMQMTTVA
                     WFGVAAFAMAVLFSLLTLPVELNASSRAMTLLTTMGIVDRTEHGQARSVLTAAAFTYV
                     AGLAAAVLNLLYYLMLVTGMGRSRD"
BASE COUNT          434 a          692 c          788 g          481 t
ORIGIN      
        1 ccaaatatca cctttcttta cgcctgtacg gtgccatgtg ctagagaata gggcaacatc
       61 ggggtgaagg acgagattct tcgctgcgct cagaatgaca agggccacct ttggccgctt
      121 gtttcctaca ggttgcagat actctccagg tcggattgtc gaggggtgct atttcgacgg
      181 caggggaaaa tgatatactg gaagggcaaa attgagccac gggggagcgc taataagttg
      241 tacgacctga ttgtaatcgg tcttggggcg atgggcagcg ccactgcata tcacgcggct
      301 tctcgcgggc acagggtgct cggcctcgac gcctacgagc gcaaccacgc caacgggtcg
      361 tcgcatggtc gcagccgcat catccgggag tcctatgcag aggggccgga gtatgtgcct
      421 ctcgtgcagc gtgcctacaa gctctggcgc gacctggagg aggagagttc ccgctctttg
      481 cttacgataa tcggcggcgt ctacataggg tcgcccggca ccgagatagt caggggcgtg
      541 ctgcggagcg cggaggagca tgggctggct tgtgattacc tctcagccgc ggagcttatg
      601 tctcgcttcc ccggctttcg tgtgccggaa gatatggtgg cggtctacga agccaacggt
      661 ggcgtgctcg atgccgaagc ctgcgtcggg gcgcatctcg acctcgcggc ccggcacggc
      721 gctgagttgc gacatggcga accggtcctg cggtgggcgg cggacggggg cggcgtgcgc
      781 gtggagacgg cggccgggag ctacacgggc gacaggcttg taatcgcagc cggtccctgg
      841 attggcggcc tgctggccga cctgtcgctg ccccttgccg tttggcgggt ctacaacacg
      901 tttttcgaac ccacacggcc tgagtttgag gttggcaggt gtcctttcta cctcctggaa
      961 gtgccggagg ggacctacta cggcctgccg tctttacccg gccagggtct caaggcgggc
     1021 cgccatgatg cgggtgaggt aagcactcca cagaacgttc gccgcaccgt ggacggcagc
     1081 gaggtagagg ctatacgctc tgtgctggag cggtacatgc cgggggccgc cggtgcgcta
     1141 aaggctacca cgacctgcgt ctacactgtc acgcccgacg cggactttgt catcgaccgg
     1201 cacccggagc acccccaggt agtttacgcc tccccatgct cagggcatgg cttcaaattt
     1261 tcgtccgcca taggtgaggt gctctgcgac ctcgcgctgg agggcacttc cgcgttcgat
     1321 gtcgcggttt tttcggcaga gcgtttcgga atcggcgcgg gtcggaagtg accaatatgg
     1381 agcgcgaacc ggagcttgga acggcacctg ctatgttcgg gtgctgagtg accaattgga
     1441 atcgcggcgc ggaaatgcaa cataaggtcc caatattgcg tataattgag taggaagcat
     1501 ggcgggtgcg tctatgccca acgatgctac ccccgacgac cacggttgtc gcataactag
     1561 aaagtagata agaggagttt ggcagatgtt cttcttcgac accagataca tatttttcgt
     1621 gatgattccg ggcattgtgc tgatgctcct ggcctcgtgg tgggtcaaga acgcctttga
     1681 gaaaggctcc aagatcccca accttcagcg gctgacgggt gcccaggtgg cgcgcatgat
     1741 cctggatagc agtggcctgg gccacgtggc ggtacagcct acccagccgc atggcttgag
     1801 cctttcctcc ggcgcgtcgc ccgacctgga cgaccactat gaccccaggg agaaggtgct
     1861 caggctctcg cccagggtgt acggcagcgc ttcggtggct gctatcgcgg tggccgcgca
     1921 cgaggctggg cacgccatac aggacgcgca gggctacgct ctcatgcggg cgcgcacggc
     1981 tatcgtgcct attgccaata tcggttcgca gcttggcggg ttcatcgtga tcctgggcgt
     2041 gatcatgcag atgaccacgg tggcctggtt cggtgtggcc gccttcgcga tggcggtgct
     2101 cttctcgctg ctgaccctgc cggtggagct gaacgccagt tcccgcgcca tgacgttgct
     2161 caccacgatg ggcatcgtgg accgcacgga gcacgggcag gccaggtcgg tgctcaccgc
     2221 cgccgccttc acctacgtgg cgggcctcgc ggccgcggtc ctcaacctgc tctactacct
     2281 gatgctagtc accggcatgg gcaggtccag ggactaactc agcaagagag actgagtcgc
     2341 aggtggaata aataatttca ccgcagaggc gcagagaacg cagaggtacg cagag
//
LOCUS       DASRWZ010000009         1770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_343320, whole genome shotgun sequence.
ACCESSION   DASRWZ010000009 DASRWZ010000000
VERSION     DASRWZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343320"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1102
                     /locus_tag="VFH60_00105"
     CDS             158..1102
                     /locus_tag="VFH60_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6312209.1"
                     /translation="MTVEENALARAGASAGQRPGISVFFPAYNDAGTIASMVIAALRT
                     CAQLTDDYEVIVVENGSTDYTVEVLEDLAETHENLRVLTHREPLGYGGALKIGFRESS
                     KELVFYTDGDAQYDAHELALLVAALRPEVDIVQGYKIERHDPIFRIVIGKIYHWTVKI
                     AFGLPVRDTDCDFRLIRKSALQRFTLTSNTGTIPVELVKKLHESGAVFAEVPVHHYHR
                     AYGRSQFFNFKRLAATARQLIRLWIELVLVPRMTGRKPVKSVKPRGSIDLTNPSLLYR
                     TEAEERAMKSTRTTASETAGSRAPGAQAPRPKAPGGKR"
     gene            1210..>1770
                     /locus_tag="VFH60_00110"
     CDS             1210..>1770
                     /locus_tag="VFH60_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6312210.1"
                     /translation="MITGGLGFIGSNLARRLVFLGADVLLVDSLIPEYGGNRRNIAGI
                     QDQLRVNIADIRTQPTMNFLVQDHDFIFNLAGQVSHLDSMTDPFTDLEINCRSQLSLL
                     EACRHNNPAVKIIFAGTRQQYGKPDYLPIDERHLMHPTDVNGINKMAGEWYHILYNNV
                     YGLRAASLRLTNTYGPRQYVKDARMGF"
BASE COUNT          351 a          600 c          534 g          285 t
ORIGIN      
        1 cgtccccacg gtgaccaatg ctgtgtatgg aggatatccg taccggccag ccgctaaccg
       61 ctaaccgcta accgctgacc actatcctcg ccactcctcc ccgcgtgtgg tataatcccg
      121 gctgacacgt aacgagggac atggagaggc accagatttg actgtagaag agaatgcgtt
      181 ggcgcgggcg ggggcgtccg cggggcagcg tccgggcatc tccgtattct tccccgctta
      241 caacgacgcg ggcaccatcg cctcgatggt aatcgcggcc ctgcgtacct gcgcccagct
      301 taccgacgac tacgaggtca tcgtggtcga gaacggcagc accgactaca cggtcgaggt
      361 gctggaagac ctggcggaga cgcacgagaa cctgcgggtg ctcacccacc gcgaacccct
      421 cggctatggc ggcgcgctca agataggctt ccgcgagtcg agcaaggagt tggtctttta
      481 caccgacggc gatgcccagt acgacgcgca cgagctggcg ttgcttgtgg cggccctccg
      541 gcccgaagtg gacatcgtgc agggctacaa gatcgagcgc cacgacccta ttttccgcat
      601 cgtcatcggc aaaatctacc attggaccgt gaagattgcc ttcggcctgc ccgtgcgcga
      661 taccgactgc gacttccgcc tgatacgcaa gtcggccttg cagcgtttca ccctcacgtc
      721 gaacacgggc acgatacccg tcgaacttgt caagaagctg cacgagtcgg gggcggtgtt
      781 cgcggaagtg cccgtgcacc actaccacag ggcctacggc aggtcgcagt tcttcaactt
      841 caagcgcctg gccgccactg cccgccagct aatccggctc tggatcgagc tggtgctggt
      901 gcccaggatg acgggccgca agccggtgaa gagcgtcaag cccaggggca gcatcgacct
      961 caccaacccc tcgctgctct accgtacgga ggccgaggag cgcgccatga agtccacacg
     1021 aacgacggcg agcgagactg cgggtagccg cgcgcccggg gcgcaagccc ccaggccgaa
     1081 agcgccagga ggtaagcgtt gaggccgggt tggacctgga acacggggcc gcttggcgag
     1141 ccgccccccg acgaagcgcc ccagcccccg cccgccgatt acgagctaat ttaccggggc
     1201 aagcgggtga tgattacggg cggcctcggc ttcatcggct cgaacctggc gaggcggctg
     1261 gtgttcctgg gcgcggacgt gctgctggtc gactcgctca tcccggagta cggcggcaac
     1321 cggcgcaata tcgcgggcat acaggaccaa ctgcgggtga acatagccga cattcgcacc
     1381 cagcccacga tgaacttcct ggtgcaggac cacgacttca tcttcaacct ggccgggcag
     1441 gtgagccacc tcgactcgat gaccgacccc ttcaccgacc ttgagatcaa ctgccgcagc
     1501 cagctatctc tgctcgaagc gtgcaggcac aacaacccgg ccgtcaagat catcttcgcg
     1561 ggcacccgcc agcagtacgg caagcccgac tacctgccga tagacgagcg ccacctgatg
     1621 caccccaccg acgtgaacgg catcaataag atggctggcg agtggtacca catcctgtac
     1681 aacaacgtct acggcttgcg tgccgcctcc ctgcgcctga ccaacaccta cggcccccgc
     1741 cagtacgtga aggacgctcg gatgggcttc
//
LOCUS       DASRWZ010000010         5209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_137782, whole genome shotgun sequence.
ACCESSION   DASRWZ010000010 DASRWZ010000000
VERSION     DASRWZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137782"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..450)
                     /locus_tag="VFH60_00115"
     tRNA            complement(375..450)
                     /locus_tag="VFH60_00115"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(415..417),aa:Lys,seq:ctt)
     gene            753..1076
                     /locus_tag="VFH60_00120"
     CDS             753..1076
                     /locus_tag="VFH60_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312211.1"
                     /translation="MEPYTRRRARTRTRATTGTTQSPTTRPRVYYPTHAHLYLGDKTP
                     LYLNWIVEGPDGDLYQVPAEVEGWAHRSSYNGPAEQLRPVEAHMGKALMEMLRVDGES
                     FQLTA"
     gene            complement(1237..2415)
                     /locus_tag="VFH60_00125"
     CDS             complement(1237..2415)
                     /locus_tag="VFH60_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6312212.1"
                     /translation="MVTYIIRRTLQALLMTLFGAFFFYTLLVYVLPWGPVNAYRRAAQ
                     EVRDYNITEPFSRILNSNGKPVGPRQPTPLFTMREMEEAYSLDKPWPLSFFAWLYDPE
                     DTMTYTRRLFLEQQKGLDIRIGPLHITGDGALLGNWGYSTYVRQGVAVQEVVSGRWGN
                     SAVLVGLGLLFSVLIAVPVGMWSAVRAGSKVDHTITFFSFAGQSMPPFVLGMLLIFAA
                     AVIPYQLHHNQGWTWLPYLPPGLTHDLDQETNWLNRLYHLVLPVSALVMSQVAWLSRH
                     VRFSMLDVLSKEYVRTAQAKGLRQWRVLWRHAFRNASIPFITSIALAIPGVLTGVVVI
                     ERLFAYPGMGDILFSAMNIRPDIPIILTVVVIMVFLIGIANLVADILYAVVDPRVGGG
                     "
     gene            complement(2467..3537)
                     /locus_tag="VFH60_00130"
     CDS             complement(2467..3537)
                     /locus_tag="VFH60_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6312213.1"
                     /translation="MSASTTSPTIARTDLDVSASGRARLAPSGQWSVAWRHFRRRRMG
                     MVGLVMLVTLVLMAIFVPVLSPFDWQATNTATMWNQPAGTVDPEGRVHWLGTTERGAD
                     LLELLFVALRTTLVVALPAALLSVLIGSALGAVAGYYGGLLDAIVMRVTDLLLAIPAL
                     PAYVILGQYLRWTVFRYADLGSGSDSMSAWASATLTVTVMVFTLLGWMGVCRLVRAQV
                     LSLRQQAFIEAARALGASNRRIIFRHLLPNALAPILVASIFLVGEFMIALSILSYIDI
                     GVNEGTFSVNNGTGGQPSLGSMLALSNYNGSTWFLANLNPLESMRAYTIILPCLLLLL
                     IVLSINYIGDAARYALDPHRQS"
     gene            complement(3598..4860)
                     /locus_tag="VFH60_00135"
     CDS             complement(3598..4860)
                     /locus_tag="VFH60_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312214.1"
                     /translation="MNGPQIRSRLGAGLAGAMRREACEAVPDNIDLWPQIEARLRARR
                     SSGRLQGFVRHLSSMAGGLPPATGAQRSQRLGRNAPAMMPLFVLVLVAWGVVAFVIGR
                     QTGVRAPELSVYRDGGTQSSAFSGTELASDANGDMLLERVLDTARLEDVRGAGLVHQV
                     GLSRQAGGCIAQIKQTYADANRLIIGMTAQAPGTSGGQPRMYGGIVSLGGAEYDGRNP
                     LLLYETSTSNSSGTAARLLVYDLSLLAEVPAQLNVVVEVYMSVGSASDQPSNPCPRSF
                     RFNLAVPVNAAGTHVVQLDRPVTVAGETVTLEKIISTPSATQAILRVGAGARHSGKWD
                     YMSVGIAQLGSANEPGLDAETDPPATSMALFNGRRSLELAQRDLASDGGTWTLKVNYL
                     EKQASPDSNSAAERLRGPWTFQFEVPAR"
     gene            complement(4857..>5209)
                     /locus_tag="VFH60_00140"
     CDS             complement(4857..>5209)
                     /locus_tag="VFH60_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6312215.1"
                     /translation="ENALHEDDAALARLDPALEEGIHSAEVSAAISAALAQLPPQQRA
                     AIVLRYYLGMNEAEMSALLNCAPGTGRWHLHRARQRLKTLLPAWVRGMFEEPQDQSSS
                     PLPLVARLPNGDTP"
BASE COUNT         1044 a         1585 c         1631 g          949 t
ORIGIN      
        1 gcctctccaa caggggtgtg gtgagtggct acccctgcgg aggcaccaac tccgccactg
       61 gagcaccaga accgtgtgat acagcatcca ggccatactt cagggtgaac aataacgtca
      121 cgaggggaca gactgccaag attgtggctg agaccttctt ccctaactgc caaactccag
      181 taagtagata gagtagaact ggctggagaa gacgtagata cgcaaacaag aaggaggcca
      241 gattgggcct ccttcttgtt tgcggttctt catttttacg agtatcacct ataggagcgc
      301 tgtgctaacg ggaatcttgc acctacgatg gtactcccta aagtcatcat ctctatttac
      361 gcacaaacgg aagttggtga accgcggggg actcgaaccc tcaacccact gattaagagt
      421 cagttgctct gccaattgag ctagcggtcc atttcaggta cacactattg taacaaaaag
      481 tgcggccggt gtcaaagtct ttttggtgcc gaaacttgcc cttcactgaa agattaccct
      541 tcagccagca cccacatcct ctcagctacc ggaactgacg agcaatagcg tgtattgact
      601 attattcacc cgcttttggc ccgattttag ccaatatgga cataatgggg ccttcttgtt
      661 gcttataatt gggtatggtg gttgtgggta cacgacgtac ggcgcggcca cccagctact
      721 caatggcgag agcgacgaga ggagatgcca atttggagcc atacactcga cggcgagcac
      781 gaacacgcac acgggcgacg acgggtacca cgcagagtcc gaccacccgc ccacgggtct
      841 actaccccac ccacgctcac ctctacctgg gcgacaagac gcccttgtac ctcaactgga
      901 tcgtggaggg gccggacggc gacctctacc aggtaccggc ggaagtggag ggctgggcac
      961 accgcagcag ctacaacggc cctgccgaac aattgcggcc ggtagaggcc cacatgggta
     1021 aggccctcat ggagatgttg cgcgtggacg gagagtcgtt ccagctaacc gcgtagccct
     1081 attcgcctga cataagtaac caagcgtata aagtagtcaa gaaataagag caccggccgc
     1141 gttcccgtcg cagggggtac cgccagtgct cttattcgtg gtcgagcctc ctgtttggcg
     1201 tgatgcgggt tgcagtgcgg acagcggcac tcagtactac ccgccgccca cccgcggatc
     1261 gaccaccgcg tacaagatgt ccgcgaccag gttggcgatg cctatcaaga agaccatgat
     1321 caccacgacc gtgaggatga tcggtatgtc cggcctgatg ttcatggccg agaacaatat
     1381 gtcgcccatg ccagggtagg caaagagcct ctcgatgacc accacgcccg tgagcacgcc
     1441 cggtatggcg agggctatgc tggtaatgaa gggtatggag gcgtttcgga aggcgtgccg
     1501 ccacagcacc cgccactgcc tcaggccctt tgcctgggcg gtgcgcacgt actctttcga
     1561 gagcacgtcg agcatagaga agcgcacgtg ccgggaaagc caggctacct gcgacatgac
     1621 caacgcactc accggcagca ccaggtggta gaggcgattg agccagttgg tttcctggtc
     1681 caggtcgtgg gtgaggccgg gcggcaggta gggcagccag gtccaaccct ggttgtggtg
     1741 taactggtag gggattacgg ccgccgcgaa gatcaacaac atgcccagca cgaacggggg
     1801 catcgattgc cccgcgaacg agaagaaggt gatcgtgtgg tccaccttgg accccgccct
     1861 gaccgcgctc cacataccca cgggcacggc tattagcacg ctgaacagca ggcccagacc
     1921 caccagcacg gcagagttgc cccacctgcc gctgacgacc tcctgtacgg ccactccctg
     1981 gcgtacgtag gtagaatagc cccagttccc cagtaacgcc ccgtcccccg taatgtgcag
     2041 cgggccaatc cgtatgtcga ggcccttctg ctgctccagg aacagcctgc gagtgtaagt
     2101 catcgtgtcc tcggggtcat acagccaggc gaagaagctg aggggccagg gcttgtccag
     2161 gctgtatgcc tcctccatct cgcgcatggt gaacaagggg gttggctggc gaggcccgac
     2221 cggtttgccg ttcgagttca ggatcctgct gaatggctcg gtgatgttgt agtcgcgcac
     2281 ctcttgggcc gcgcgccggt aggcatttac aggcccccac ggaagcacgt agaccaggag
     2341 cgtgtagaag aagaacgccc cgaacaacgt catgaggaga gcctggagcg tgcgccggat
     2401 gatgtatgta accatatcgc ccctttgctg tttactctat agcggcaggc ccgaaatgta
     2461 agctggttag ctttgcctgt gggggtcgag ggcgtagcgt gccgcgtcac cgatatagtt
     2521 gatggagagc acgatcagca gcagcaggag gcaaggcaag atgatcgtgt aggcccgcat
     2581 cgactccagc gggttcaggt tggcgagaaa ccacgtgctg ccgttgtagt tggatagcgc
     2641 gagcatgctt cctagagacg gctgtccccc cgtgccgttg ttgacgctga aagtgccctc
     2701 gttcactccg atatcgatgt atgagaggat agatagcgca atcatgaatt caccgacgag
     2761 gaagatgctt gcaaccagga tgggggccag tgcgttgggc aacaggtgcc tgaagatgat
     2821 gcgcctgttg ctcgcaccca gggcgcgcgc ggcctcgatg aaagcctgtt gccgtagcga
     2881 cagcacctgc gcccgcacga gcctgcacac ccccatccag ccgagcaggg tgaataccat
     2941 caccgttacg gtcagcgtgg ccgacgccca ggcgctcatc gaatcgctgc cagagccgag
     3001 gtcggcgtac cgaaagacgg tccaccgcag gtattgcccg agtatgacgt aagcaggcaa
     3061 agccgggatg gccagcagca ggtccgtgac gcgcatgaca atagcgtcca acaagccgcc
     3121 gtagtagccg gcgacagccc ccagggccga gcctatcaac accgacagca aggcggctgg
     3181 cagagccact accagcgtcg tacggagcgc cacgaagagt agctctagca ggtccgcgcc
     3241 gcgctcggtg gtgccgagcc agtggacgcg cccctcgggg tctaccgtcc cggcgggttg
     3301 gttccacatg gtggccgtgt tcgtggcttg ccaatcgaaa ggcgaaagta cgggcacgaa
     3361 gatcgccatg agcacgagcg ttacgagcat caccagcccc accatgccca tgcgacgcct
     3421 gcgaaagtgc cgccaggcca ccgaccactg gccggacggg gcgagacgcg ccctgccgga
     3481 agcggagaca tcgaggtcgg tgcgcgctat agtaggggaa gtcgtggacg cggacatact
     3541 ggcccttcct gttgaaacaa gggtattgcg tgtaattgga gatgtggtgc cagtggttta
     3601 gcgggcgggc acctcgaatt ggaaggtcca cggcccgcgc aaacgctctg ccgccgaatt
     3661 tgaatccggc gatgcctgct tttcgagata gttcaccttg agggtccagg tgccgccgtc
     3721 cgaggccagg tctctctgtg ccagctcaag ggagcgccgc ccgttgaaaa gggccatcga
     3781 cgtggctggc gggtcggtct ctgcatcaag gcccggctcg tttgccgagc cgagttgggc
     3841 aatacctacc gacatgtagt cccacttgcc ggagtggcgc gcacctgctc ccacgcgaag
     3901 gattgcctga gtcgcggaag gcgtgctgat tattttctcc agcgtgacgg tctcacccgc
     3961 tacggtcact ggcctgtcga gctgtaccac gtgggtgcct gccgcgttga caggcacggc
     4021 caggttgaac cggaaggagc gcgggcacgg attcgagggc tggtctgaag cggaccccac
     4081 cgacatgtag acctctacga cgacgttcaa ctgagcaggt acctccgcca gcagggacag
     4141 gtcgtatacc agcaggcggg cggcggtacc ggacgagttg ctggtgctcg tctcgtacag
     4201 cagcagcggg ttcctgccgt catattccgc cccgcccagg ctaactatgc ccccgtacat
     4261 cctgggctgc ccccccgatg tgccgggtgc ctgggcggtc atgcctatta tcagccggtt
     4321 tgcgtctgcg taagtctgct tgatctgcgc gatgcagccg cctgcctgcc ggctcaagcc
     4381 cacctggtgc accaaccctg ccccgcgcac gtcttcgagc cgggcggtgt cgagcacccg
     4441 ctctaacagc atgtcgccgt tggcgtcgga agccagttcg gtgccgctga aggcgctgct
     4501 ctgcgtgccg ccgtcgcggt agaccgacag ttccggggcg cgcacgcccg tctgccggcc
     4561 gatgacgaag gcgaccaccc cccacgctac cagcaccagc acgaacaacg gcatcatagc
     4621 cggggcgttg cggcccaggc gttgcgagcg ttgcgctccg gttgcgggcg ggaggccacc
     4681 cgccatagag gagaggtgac gcacgaagcc ctgtagcctt ccacttgagc ggcgagcgcg
     4741 caggcgtgcc tcgatctgcg gccagaggtc gatgttgtcg ggcaccgcct cgcatgcttc
     4801 cctgcgcatg gccccggcaa gtccggctcc cagcctggag cgaatttgcg gcccgttcat
     4861 ggcgtgtctc cgttgggcag gcgggctacg aggggtagcg ggctggagct ttggtcttgc
     4921 ggctcctcga acatacctcg cacccaggcg ggcaggagcg tcttcaagcg ctggcgcgcc
     4981 ctgtggaggt gccacctgcc ggtgccgggc gcgcagttga gtagggccga catttccgcc
     5041 tcgttcatgc ccaggtaata gcgcagcacg atagcggcac gctgctgtgg cggcaattgt
     5101 gcgagggccg cgctgatggc ggcactcact tccgccgaat ggatgccttc ctcgagagcc
     5161 gggtccaggc gcgcaagagc agcgtcgtct tcgtgcagag cgttctcca
//
LOCUS       DASRWZ010000011         3892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_206259, whole genome shotgun sequence.
ACCESSION   DASRWZ010000011 DASRWZ010000000
VERSION     DASRWZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206259"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..3576)
                     /locus_tag="VFH60_00145"
     CDS             complement(208..3576)
                     /locus_tag="VFH60_00145"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HET6312216.1"
                     /translation="MRRYLPVLLAALTLLCAWLALGLPGAFTAKASTSHAGPLTALDL
                     AGYAAEAGPGPNLDAAPRSPERGSPLSPEATDPPILPPGFVQVPLATGLTYPTSFAFA
                     PDGRVFIAEKGGMVRVLKAGGVLVAPVLDLSAQVNTYWDRGMMTVAVDPDFETNKYIY
                     VYYTYEPDPNADLESDAYKGPKTNKLARYTLQGDVALTATEKILLGSVAGTVAVQSCN
                     NYPAGSDCIPSDGPSHIGGTIRFAPDGSLFLSTGDAADFANINDNALRVQSLNSLAGK
                     LLRIDRDTGQGFLDNPFSSGNVNAIQSKVWAYGLRNPFRFNFRPDTTVPYVGDVGMDA
                     WEELDVVKPGLNYGWPCYEGPAPHPGYAAKPLCGQLQASAVQTPLLSLKHSAGNWCAV
                     GGTFYTGTTYPAQYQGAYFFADCVYRYIDYLLVDANNNLVSGPHRFGYLRYNVSEFAA
                     GGPTQLEMGADGNLYILETVFGTLSRIEVDDPKAPPQGTTYVSDLAWTSVTNGYGVLE
                     RDRSNGGSTPFDGQTITLNTVTYSKGVGVHANSDIKLYLGKSCTNFTSDVGVDDEVGA
                     YGSITFEVLADGGSLFQSGIMTGSTATRNVSVDVTGKEELVLSVRNGGDNSAYDHGDW
                     AGTRVTCKGVPAVAGTVPEDGEAGVLPGANVTVTFSKPMDASTFTGSNVVLTRQGSST
                     PVAATLNYNQAARTLSIDPVNDLQATSNYTVVVKGGPNAVKDQDGLGLYADVVWSFST
                     SSRPAPVISLPLPDLRVKVGDVVTYTGYATDAEDGPIPASALSWDVSICHGCPGQGSH
                     THPWQSGTGASGSFVVPDHGDNSYLVIVLTARDSDGLTASTSVEVRPQMVEITMSSVP
                     PGLQLVYDSYQATTPFTRTAAAGSTHSLWAPSPQGGHLFEGWLDGGAQSHNVQVGVTN
                     TTYTALFSTPVPSSTPMSTATPTPLACDVQFTDVPQGSTFYSFVRCLACGGVLGGYAD
                     GTFKPNVGITRGQLAKLVSNAAGYDEGISGQTFSDVPPGSTFYEYVERIVGRGVVGGY
                     ADGTFGPGNSATRGQIAKVLSNAAGFSDAPASPSFSDVGSSDPFYPYVERLASRGIIG
                     GYADGTFGPGNPATRGQVAKMVANTFFPECAPPQ"
BASE COUNT          716 a         1172 c         1266 g          738 t
ORIGIN      
        1 agaggacgcg ctgagattag ggaaggttta atagagccaa atgggcagca tcgtacagta
       61 gatgctgccc atttgcacat gacaccctca attcttctct gcgcgccctc agcgtcctct
      121 gcgcctctgc ggtgaaccgt actggttcct cttggcgcgt tcctggcgtt cttggcgtct
      181 tggcgggttt cgtctcctct cctgttgcta ttgtggaggc gcgcattcgg ggaagaaggt
      241 attggctacc atcttggcga cttgccccct ggtggcgggg ttgccggggc cgaaagtgcc
      301 gtctgcgtac cccccgatta tgccacgcga ggccagccgc tcgacgtagg ggtagaaggg
      361 gtcgctgctg cccacgtcgg agaaggacgg gctagcaggc gcatctgaga agcccgccgc
      421 gttcgagagg actttggcga tctggcctct ggtggcggag ttgccggggc cgaacgtgcc
      481 gtccgcatac ccgcccacta cgcccctacc cacgatgcgc tctacatact cgtagaaggt
      541 gctacccggc ggcacgtccg agaaggtctg ccccgaaatg ccttcgtcgt agcctgccgc
      601 gttggacacc agcttggcta gctgcccgcg tgtgatgccc acgttgggct tgaaggtgcc
      661 gtccgcgtac ccaccgagca cgcccccgca ggcaagacac cgcacgaagc tgtagaaggt
      721 cgaaccctgc ggcacgtcgg tgaattgcac gtcacaggca agcggcgtgg gagtggctgt
      781 agacataggg gtgcttgagg gcacgggcgt gctgaagagc gccgtgtagg tggtattggt
      841 gacccctacc tgtacgttat ggctctgggc gccgccgtcc agccagccct cgaaaagatg
      901 gccgccctgg ggagaaggcg cccacaggct gtgcgtggaa ccggctgccg ccgtgcgcgt
      961 gaaaggtgtc gtggcctggt agctatcgta caccagttgc aagcccggag gcacggacga
     1021 catggtgatc tccaccatct gcgggcggac ctctaccgag gtggacgcgg tcagcccgtc
     1081 gctatccctg gcggtcagca cgatcacaag gtagctgtta tcgccgtggt cgggcacgac
     1141 gaagcttccg ctcgcgcccg ttcccgactg ccacgggtgc gtatgagaac cctgtccggg
     1201 gcaaccatgg cagatcgaca cgtcccaact cagcgcgctg gccgggatgg ggccgtcttc
     1261 cgcgtcggta gcgtagccgg tgtaggtgac tacgtcgcct accttgacgc gcaagtccgg
     1321 cagagggagg cttatcaccg gggcggggcg ggagctagtg ctgaacgacc acaccacgtc
     1381 ggcgtatagc cccaacccgt cctggtcctt cacggcatta ggcccgccct ttaccacgac
     1441 cgtgtagttc gaggtagctt gcaggtcgtt caccgggtct atggaaaggg tgcgcgccgc
     1501 ctggttgtag ttgagcgtgg ctgccaccgg cgtggaggaa ccttgccggg tcagcacgac
     1561 gttcgagccg gtgaaggtgg acgcatccat aggcttgctg aacgtgacgg ttacgttagc
     1621 acctgggagg acgcctgcct cgccatcctc cggcaccgta ccggctacgg cgggcacacc
     1681 cttgcatgtt acgcgggtgc ccgcccagtc gccatgatcg taggcgctgt tgtcaccacc
     1741 gttgcgcacc gacagcacca actcttcctt gcccgtgaca tcgacactca cattcctggt
     1801 agcggtggaa ccggtcataa tgccgctctg gaagagggat ccgccgtcgg ccagcacctc
     1861 gaacgtgatc gagccgtatg cacccacctc gtcgtccacg cctacgtctg aggtgaagtt
     1921 ggtacagctt ttacccaggt acagcttgat gtccgagttg gcgtgcacgc ctacgccttt
     1981 ggagtaagtg accgtgttga gggtgatcgt ctggccgtcg aagggcgtgc tacctccatt
     2041 gctcctgtct ctctcgagaa ccccgtagcc gttggtcacg gaagtccatg ccaggtcgct
     2101 cacgtaagtg gtgccctggg gcggggcttt tggatcgtct acctcaatgc ggctcagggt
     2161 gccgaagacg gtctccagga tgtacaggtt gccgtccgcg cccatctcca gttgggtggg
     2221 gccgcccgcc gcaaactcgc tcacgttgta gcgcaagtag ccgaaccggt gcggcccact
     2281 caccaggttg ttgttggcat cgacgagcag gtagtcgatg tagcggtaaa cgcaatcggc
     2341 gaaaaagtag gccccctggt actgcgccgg gtaggtcgtg cccgtgtaga acgtaccgcc
     2401 gaccgcgcac cagttgcccg ccgagtgctt caggctcagc aagggggttt gcaccgcgct
     2461 agcctgcaac tggccgcaca aaggcttggc ggcataaccg gggtgcgggg ctggcccctc
     2521 gtagcagggc cacccgtaat tgaggccagg cttcacaaca tcgagttcct cccaggcgtc
     2581 catccccacg tctcctacgt acgggacggt ggtgtcaggg cggaagttga accggaaggg
     2641 gttgcgcaag ccatacgccc ataccttcga ctgtatggca ttgacgttgc cgctggagaa
     2701 ggggttgtcg aggaaaccct gccccgtgtc tctatctatc ctcaggagct tcccggcaag
     2761 ggagttgaga ctctggacgc ggagcgcatt gtcgttgatg ttggcgaaat cggcggcgtc
     2821 ccccgtcgag aggaacaacg aaccgtccgg ggcaaacctg atcgtgcccc cgatatgaga
     2881 cggcccgtcg ctgggtatgc agtcgctgcc cgccgggtaa ttattgcacg actgtacggc
     2941 gacagtgccc gccaccgagc cgaggagtat cttctccgtg gccgtcaggg ccacgtcgcc
     3001 ttgcagggtg tagcgtgcga gtttgttcgt ctttgggcct ttgtacgcgt ccgactccag
     3061 gtccgcgttg ggatcaggct cgtacgtgta atagacgtag atgtacttgt tcgtctcgaa
     3121 gtcggggtca accgccacgg tcatcatgcc ccggtcccag taggtgttca cctgcgcgct
     3181 gaggtcgagc accggggcga ccaacacccc accggccttc agcacgcgca ccatgccccc
     3241 cttttcagcg ataaagacgc ggccatcggg cgcgaacgcg aaagaggttg ggtaggtaag
     3301 cccggtagcg agagggacct ggacgaaccc cggcggcaaa ataggagggt cggtagcttc
     3361 gggagacagt gggctgccac gttccggcga gcgcggcgca gcgtccaggt tcggcccggg
     3421 accggcctcg gctgcgtacc cagcgaggtc aagcgcggtc agcggaccgg catgagaggt
     3481 cgacgcctta gcagtaaagg cccccggaag cccaagggcg agccaggcgc agaggagtgt
     3541 gagtgcggca agaaggaccg gtaggtaacg gcgcacgcgt gaatgacccc tttatgatga
     3601 acgagacggc acggtaatac ccgcctgaca gttaaccgcc ggaccgggac acggcaccaa
     3661 cgggaggaca agcacatgaa acgtggagac ataccactca ggtaagagta ccattcaagg
     3721 ggcgtcacgt caagccgtct gacggagcgg gcggtgtgta gtacaagtga tgtaaagtca
     3781 agaacgttcc aactggtgaa actacagagg cggcaactat ctttccgaac atgccgtgtt
     3841 gtaggagcac ggttgccgta ctcctactgc ttgttagggg gctatctgtt ag
//
LOCUS       DASRWZ010000012         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_206313, whole genome shotgun sequence.
ACCESSION   DASRWZ010000012 DASRWZ010000000
VERSION     DASRWZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206313"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFH60_00150"
     CDS             complement(<1..891)
                     /locus_tag="VFH60_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6312217.1"
                     /translation="MRILYFTQYFPPEVGATQTRAFEMSRYLASRGHHVTVVTEVPNH
                     PSGIIPPAYRGRLSERRVEHGVDVFRLWVWTSPEKTFATRMRFYLSYMGMAALAGSLV
                     RGRYDLVYATSPPLFVGAAGLVASLVRRVPLVFEVRDLWPESAVALGELSNSRAIALA
                     EKLERLLYARARRVVTVTQGIRTRLLQRGIPPEKLALIPNGANTDLFQHDAEGAARLR
                     RELGLEEAFVVLYAGVHGIAQGLETLLEAARLLQDEPRVQFLMLGEGPRKAALVQQRE
                     SLGLTNLRMLPEVATTSMPAY"
     gene            complement(894..>2162)
                     /locus_tag="VFH60_00155"
     CDS             complement(894..>2162)
                     /locus_tag="VFH60_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HET6312218.1"
                     /translation="PDSEDPVGKHDESYYVSTTRALLEDSVRLRLRSDVPLGAHLSGG
                     LDSSSVVALLSRRLHESGERVRTFAGAFAEGPAYDERKYVDEVVRQYGTDHSETLPTA
                     EDLRRLFERIVWHMDEPAAGPGILLQWAVCRITNDAGVIVINGGQGGDEAWAGYFGYV
                     PAYFSSLLRAARKHPRLLLDVLKSGAVLLSRSNTRMTLLKTLVRGRKARLRASGELGP
                     WAGPAFRKASANGEKGKPSASTPSHRGKRTPLGSVMYYDLKWYLPALLQVEDRTSMAF
                     SLESRAPLLDYRLIELASRVPSPLRMKHLDMKHIFKAAVKDVLPPSISRRTDKMGMPT
                     PVDLWFRESLGGWVREQLDSPEVRSFGLLDPDYVLKALEEHQSGARDRSLDLWKMLNL
                     VTWWRLHIAKTAPIPEPQVVNRQPEPSLAF"
BASE COUNT          360 a          685 c          747 g          370 t
ORIGIN      
        1 ataggcgggc atggaggtgg tcgccacctc cggcagcatc ctcaggttgg taaggcccag
       61 cgactcgcgc tgttgtacca gagccgcctt ccttggcccc tcacccagca tgaggaattg
      121 gactcgcggt tcgtcctgca agagcctcgc cgcttcgagc aacgtctcca ggccctgtgc
      181 tatgccgtgc acgccagcgt atagcaccac aaaagcttct tccaggccaa gctcgcgccg
      241 cagccgggcc gcaccttcgg cgtcgtgctg gaaaaggtcg gtgttggccc cgttgggtat
      301 gagggcgagt ttctccggcg ggatgcctcg ttgcagcagg cgggtgcgga tgccctgcgt
      361 cacggtcact acgcggcggg cgcgggcgta gagcagccgt tccagcttct cggccagggc
      421 aatggcgcgc gagttgctca gctcacccag ggccacggcg gactccggcc acaggtcgcg
      481 cacttcgaac acgagaggta cccggcggac cagcgaggct accaggcccg cggcccccac
      541 gaacaggggc ggcgaggtgg cgtaaacgag gtcgtagcgc ccgcgcacca ggctgccggc
      601 gagcgcggcc atgcccatgt agctgagata gaagcgcatg cgtgtggcaa aagtcttttc
      661 gggggaggtc cacacccaga gccggaacac gtcaactccg tgctccacgc ggcgttcgga
      721 gaggcggccc cggtaggcgg gtgggatgat gccggagggg tggttgggca cttcggtcac
      781 taccgtgacg tggtgcccgc gggaggccag gtagcggctc atctcgaagg cgcgcgtctg
      841 ggtagcgcct acttccgggg ggaaatactg cgtgaagtaa aggatgcgca tgctcagaag
      901 gccaggcttg gctcaggctg cctgttcacc acctggggtt cggggatagg ggccgtcttc
      961 gctatgtgca gccgccacca ggtcacgagg ttgagcatct tccacaggtc gagcgaccta
     1021 tcgcgcgcgc ccgattggtg ctcctccagc gccttgagca cgtagtcggg gtccagcagc
     1081 ccgaacgacc gcacctcagg cgagtcgagt tgctccctca cccagccgcc caggctttcc
     1141 cggaaccata gatcgaccgg cgtgggcata cccatcttgt cggtgcgcct ggagatggag
     1201 ggcgggagca cgtctttgac ggcggccttg aagatgtgct tcatatcgag gtgcttcatc
     1261 cttagggggc ttggcacgcg tgacgcaagc tcgatgaggc ggtagtccag caagggcgct
     1321 cgcgattcca gggagaaggc catgctggtg cggtcctcca cctgcagaag cgcgggcagg
     1381 taccacttga ggtcgtagta catgacagag ccgagcgggg tccgcttgcc tcgatgcgag
     1441 ggcgtagagg cagagggctt ccccttctcg ccgttcgcgg acgccttacg gaacgcagga
     1501 ccggcccacg gccccagctc gccggaggcc cttagccggg ccttcctacc acgcaccagc
     1561 gtcttcagca gggtcatgcg cgtgttggag cggctgagca atacggcccc gctcttgaga
     1621 acatccagca ggagcctggg gtgcttgcgg gcggctctga ggaggctgct gaagtaggca
     1681 gggacgtagc cgaaataccc cgcccaggct tcgtcgccgc cctgcccgcc attgatgact
     1741 atcaccccgg cgtcgttggt gatgcggcac acggcccatt gcagcaaaat gccgggtccg
     1801 gcggcaggct cgtccatgtg ccatacgatg cgctcgaaca ggcgtcggag gtcctcggcg
     1861 gtgggcaagg tctcgctgtg gtcggtgccg tactgcctca ctacctcatc gacgtatttg
     1921 cgctcgtcgt aggccggtcc ctcggcgaag gccccggcga aagtgcgcac ccgctcgccg
     1981 ctctcgtgca gcctgcggct aaggagggcg accacggagc tactgtcgag gccgccgctg
     2041 aggtgcgcgc cgaggggcac gtccgagcgc aggcgcaggc gcaccgagtc ttcgagcagg
     2101 gcgcgcgttg tggatacgta gtagctctcg tcgtgcttgc caacaggatc ttccgaatcg
     2161 gg
//
LOCUS       DASRWZ010000013         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_412066, whole genome shotgun sequence.
ACCESSION   DASRWZ010000013 DASRWZ010000000
VERSION     DASRWZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412066"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..3792)
                     /locus_tag="VFH60_00160"
     CDS             complement(154..3792)
                     /locus_tag="VFH60_00160"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET6312219.1"
                     /translation="MHIPRHFTDAVDPYTKLSFVPRTSKISNPDGTVVFKAENVMVPD
                     GWSQVATDILAQKYFRRAGVAAVLKRVPEEGVPEWLWRSEPDEEALARLPKSEQYGGE
                     TDSRQVFHRLAGCWAYWGWKGGYFTSEDDARHFYDEMCFMLAAQYAAPNSPQWFNTGL
                     HWAYGIAGPAQGHYYFDPVAGELKKSTSAYERPQPHACFIQSVQDDLVNPGGIMDLWV
                     REARIFKYGSGTGSNFSALRGENESLSGGGRSSGLISFLKIGDRAAGAIKSGGTTRRA
                     AKMVIVDIDHPDIEKFIDWKVVEEQKVAALVAGSIACRKHLNTIMQAWAEADQATEAD
                     KAPKTKALKKAVRAAREGGVPDGIIARAIGLAEQGETTFDFPIYDSDWEGEAYLTVSG
                     QNANNSVRMSNGFVDAVQKDLDWELTRRTDGKPAKTLKARSLWDRIGKAAWASADPGV
                     QFDTTINEWHTCPSDGRINGSNPCSEYMFLDDTACNLASLNLMHFLGDDPDKVLDIEA
                     FRHANRLWTLVLEISVAMAQYPSAEIALLSHRYRTLGLGYANLGALLMRLGIPYDSPQ
                     AYAFCGAISAIMTGVAYATSAEVASELGPFERYEANREPMLRVIRNHRRAAHYAPDKE
                     YEGLTVKPSGISPAHCPPGLHAAAKQAWDEALELGEKSGYRNAQVTVIAPTGTIGLVM
                     DCDTTGIEPDFALVKFKKLAGGGYFKIINQSVPPALQYLGYSPEQIEDIISYVVGHKS
                     LAGSPAIDHEALRAVGFDDEAIARVEAAMPSAFDISFAFSPFVLGEEFCKETLGLTDE
                     QINSREGILPHLGFSKADIYAANDYVCGTMTVEGAPHLQDEHLPVFDCANRCGRRGTR
                     YLSPSSHLHMMAAAQPFITGAISKTINMPADTTISDVEDVYMKSWRLMLKAVAIYRDG
                     SKLSQPLNSFTAEDEDEEEEEVAAGIPIVQRVQQLAATGAEAAVREVRRLKLPNRRSG
                     YTQKAVISGHNVYVRTGEYEDGRLGEIFIDMHKEGAAFRSVMNCFAIAVSLGLQYGVP
                     LDEYVDAFILTRFEPSGRVMGNDHIKNATSVIDYIFRELAICYLDRLDLAHLPEGYDT
                     NRGDTVPDLTKKPQVNIGPTTVTGMSPHLQPLKNGNGTGTGTGNGNGHSHTNGHAEAA
                     KVEAPTAVLTQTMVMVKAEPTRSEQAAEAKLKGYEGEGCPECGNFTLVRNGTCLKCDS
                     CGSTTGCS"
     gene            complement(4014..4445)
                     /gene="nrdR"
                     /locus_tag="VFH60_00165"
                     /pseudo
     CDS             complement(4014..4445)
                     /gene="nrdR"
                     /locus_tag="VFH60_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
BASE COUNT          744 a         1454 c         1567 g         1000 t
ORIGIN      
        1 gcagagccag catcttggat tcttcgctgc gctcagaatg acaggggcca ctctggtccc
       61 gcacgcgcgg cactttgcag atacttcctt gtaaggacaa acgtggaggc agggcgccgt
      121 gccctgcctc cacgtttgac gaagctaacg gaactagcta caacccgtcg tgctcccgca
      181 gctatcgcac ttcagacagg tgccgttgcg caccagggtg aagttgccgc actccgggca
      241 gccttcgcct tcgtagccct tgagcttggc ctcggcggcc tgctcgctcc gggtcggttc
      301 tgctttgacc atcaccatcg tctgcgtcag cacggcggtg ggggcctcta ccttggcggc
      361 ttcggcgtgg ccgttggtgt ggctatggcc gttaccattt ccagtgccgg tgccagtccc
      421 gttgccgttc ttcaacggct ggaggtgggg cgacatgccg gtgaccgtcg tggggccgat
      481 gttcacctgt ggcttcttgg tcaggtctgg caccgtgtcg cccctgttgg tgtcgtagcc
      541 ctccggcagg tgcgccaggt cgaggcggtc gaggtagcag atagctagct cgcggaagat
      601 gtagtcgatc accgaggtgg cgttcttgat gtggtcgttg cccatcacgc gcccgctcgg
      661 ctcgaaccgg gtcaggatga aggcatcgac gtactcgtcc agcggcaccc cgtactgcaa
      721 gccgagggac accgcgattg cgaagcagtt catcaccgac cggaaggccg cgccctcctt
      781 gtgcatgtcg atgaagatct cgcccagccg gccgtcctcg tactcgccgg tgcgcacgta
      841 gacgttatgg ccggagatga ccgccttctg ggtgtagccg gagcggcggt tgggcagctt
      901 caagcggcgc acttcgcgca cggccgcctc ggcacccgta gcggcaagct gctgcacgcg
      961 ctgcacgatg gggataccgg ccgcgacttc ctcttcctcc tcgtcctcgt cttcagccgt
     1021 gaaggagttg aggggctggc tcagcttgga gccgtcgcgg tagatcgcca ccgccttgag
     1081 catcagccgc cacgacttca tgtagacgtc ttccacgtcg ctaatcgtgg tgtcggcggg
     1141 catgttgatc gtcttggaga tcgcgccggt gatgaacggc tgggcggccg ccatcatgtg
     1201 caggtgggac gacgggctga ggtagcgggt gccccggcgg ccgcagcggt tggcgcagtc
     1261 gaacacgggc aggtgctcgt cctgcaggtg cggcgcgccc tccaccgtca tcgtgccgca
     1321 cacgtagtcg ttggcggcgt agatgtcggc cttgctgaag ccgaggtgcg gcaggatgcc
     1381 ctcgcggcta ttgatctgct cgtcggtgag gcccagcgtc tccttgcaga actcctcgcc
     1441 cagcacgaag gggctgaacg cgaagctgat atcgaaggcg gaaggcatgg cggcttccac
     1501 acgtgcgatt gcctcgtcat cgaagcctac cgccctgagc gcctcgtggt ctatcgcggg
     1561 agagcctgcc agggacttgt gcccaacgac gtagctgatg atgtcctcga tctgctccgg
     1621 gctgtagcca aggtactgga gcgcgggggg caccgactgg ttgatgatct tgaagtagcc
     1681 gccgcccgcc agcttcttga acttcacgag cgcgaagtca ggctctatgc ctgtggtgtc
     1741 gcagtccatg accaggccaa tcgtcccggt gggcgcgatg accgtcacct gcgcgttgcg
     1801 gtagccggac ttctcgccca gttccagagc ctcgtcccac gcttgcttgg ccgcggcatg
     1861 caggccggga gggcagtgcg ccggggatat gccggagggc ttcacggtaa ggccctcgta
     1921 ctccttgtcg ggtgcgtagt gggcggcgcg gcggtggttg cggataacgc ggagcatcgg
     1981 ctcgcggttg gcctcgtaac gctcgaacgg gccaagctcg gaggcgactt cagcggaggt
     2041 agcgtaggcc acgcccgtca tgatggcgga gatcgcgccg cagaacgcgt aagcctgggg
     2101 cgaatcgtag gggatgccga gccgcatgag cagcgcgccc aggttggcgt agccgaggcc
     2161 gagcgtgcgg tagcggtgcg acaggagcgc gatttccgcg ctggggtact gcgccatcgc
     2221 cacgctaatt tcgagcacca gcgtccacag gcggttggcg tgccggaacg cctctatgtc
     2281 gagcaccttg tccgggtcgt cgcccaggaa gtgcatcagg ttgagggagg ccaggttgca
     2341 ggccgtgtcg tccaggaaca tgtactcgga gcacgggttg gagccgttga tccgtccgtc
     2401 gctggggcag gtgtgccact cgttgatcgt agtgtcgaac tgtacgccgg ggtccgccga
     2461 ggcccaggcc gccttaccga tgcggtccca caacgagcgg gccttgaggg tcttggcggg
     2521 cttgccgtcg gtgcggcggg tcagttccca gtcgaggtcc ttctgcaccg cgtccacgaa
     2581 gccgttggac atgcgcaccg agttgttggc gttctgcccg ctcacggtga ggtaagcctc
     2641 gccctcccag tcggagtcgt agatggggaa gtcgaaggtc gtctcaccct gctcggccag
     2701 cccgattgcg cgggcgatga tgccatccgg tacgccaccc tcgcgggccg cgcgcacggc
     2761 ctttttgagc gccttggtct tgggtgcctt gtcggcctcg gtcgcctggt cagcttctgc
     2821 ccacgcctgc atgatggtgt tgaggtgctt gcggcacgcg atggagcctg ccaccagcgc
     2881 ggcgaccttc tgctcctcga ccaccttcca gtcgatgaac ttctctatgt cggggtggtc
     2941 aatgtccacg atcaccatct tggccgcgcg ccgcgtggtg ccgcccgact tgatagcccc
     3001 cgccgccctg tcgcctatct tgaggaagga gataaggccg ctggagcgcc cgccgccgga
     3061 gaggctttcg ttctccccgc gcaacgccga gaagttggag ccggtgccgg agccgtactt
     3121 gaagatacgg gcctcgcgca cccacaggtc catgatgccg ccggggttca ccaggtcgtc
     3181 ctgtacggac tggataaagc acgcatgggg ctggggtcgc tcgtaagcgg aggtggactt
     3241 cttcagctcg cccgcgacag ggtcgaaata gtagtggccc tgggccggtc ccgcgatgcc
     3301 gtacgcccag tgcaggccgg tgttgaacca ctggggagag ttgggcgcgg cgtactgagc
     3361 ggcgagcatg aagcacatct cgtcgtagaa gtggcgcgcg tcgtcctccg acgtgaaata
     3421 gccgcccttc cagccccagt aggcccagca gccggcgagg cggtggaaca cctggcgcga
     3481 gtcggtctcg ccgccgtact gttcgctctt gggcagcctg gcgagggcct cttcgtccgg
     3541 ctcggagcgc cagagccact ccggcacgcc ctcttcgggc accctcttga gcacggcggc
     3601 taccccggcg cggcgaaagt acttctgggc caggatgtcg gtggcgacct gcgaccagcc
     3661 gtcgggcacc attacgttct cggccttgaa caccaccgtg ccgtcggggt tgctgatctt
     3721 ggacgttctg ggcacgaagc tcagctttgt gtaggggtcc acggcgtcgg tgaaatggcg
     3781 tggaatgtgc atgcgagact ccttgaagcc cactgcgggc ttgtctttct tctttttcct
     3841 ggctctatcc ctgacctttc taggttccat gtctttgccc ttttgccgtt atgccgctat
     3901 gccttggctt tgttcgactt gcccggcttg tcgtcgggtc cgggcagccc tgtgaccttg
     3961 cctggtggcg cgtcctcccc ggcttgcgtg gcctcagttt cgagggaggg gtacatcacc
     4021 tcgcgcatct cctcgaggtc ggcgaagttg cggtagacgg aggcgaagcg gatgtaggcc
     4081 acctcgtcaa gctcgcgcag cttctgcatt acaagctccc ctacctgcgt agagggcact
     4141 tccgacgcgc ccaggttgaa gagttgcgcc tcgatgtcgt tcaccgccgc ctcgagttgg
     4201 tcggccgaca ccctgcgctt ggtgagggct tttgccatgc cctggcgcag cttttcgcgg
     4261 ctgtactctt cccgcctgcc gtcccgcttg accaggctca ccccgatgcg ctcgatgcgc
     4321 tcgaacgtgg tgaaacggcg cttgcagtgc ttgcactggc gtcgtcgctt caccacctcg
     4381 ccgtctgagg ccaggcggct gtcggtgact tgcgtatctt cgtggtggca atagggacac
     4441 ttcataaaat ctccatcctg ggtgaattag ctactatatg ttgtatattg cataacgtgt
     4501 gcccaagatg ttgtgtaggc acatattatg tcacaaaatg tagtgggtca agtggttttt
     4561 gcacatgttc cacaacatct tgtggattgg tgcgaaccta cagctgtttt ccacaacatc
     4621 tggtagggtt ttgcacatcc attatacaca attctggaac atttgtgcta gataccggag
     4681 atgtaataac gaaagtgatg aaggtggtca ggaggaatga gagtattcct gttggagtag
     4741 ttctgagcag gaacgggtgt acaga
//
LOCUS       DASRWZ010000014         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_617653, whole genome shotgun sequence.
ACCESSION   DASRWZ010000014 DASRWZ010000000
VERSION     DASRWZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617653"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1666)
                     /locus_tag="VFH60_00170"
     CDS             complement(134..1666)
                     /locus_tag="VFH60_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312220.1"
                     /translation="MTTHLHRVKPERRACPAGLLVALLLAWTAGATPGLADVAPPRQP
                     PGSNISPAEGTTQVRMQSEEVVLDISAGNDKAVAEVSCEFIMLNTSDTAEQMQVRFPL
                     ADPSGQGSGFGQYPEVRDFQASVDGKVVTTGIITTENPLRTETAPIRWATFDATFPPG
                     EEVRIGVRYTTDGTGNLSAQQFTYILETGAGWKDTIGSADLSVRLPYTATSENVLAYS
                     ERQTTPGGRFDGNFIRWHWDDLEPTHESNLEVTVLAPATWEAVLKARAATEVSPMDPQ
                     VWVSLAEAYQAATTSRYPIFDNDPFAALSQEAYAHAIALQPNDAQLHAEFAALMWNQL
                     AVQAFPEEGDPWIARILNEISAALALDPQNKTAQDVYKEMRASTDRPLPLGTPTLVPD
                     LPVPTPTALSPTQTGASTAEPTVSPSATGVVDQPSATASTSSPLPTTPTTRTTPADPT
                     GPAASTTAPGAPPEATATMTAVPPRPNEGGNDVLIWAVLAALGGMALGGIATTLLRRR
                     GG"
     gene            complement(1763..2920)
                     /locus_tag="VFH60_00175"
     CDS             complement(1763..2920)
                     /locus_tag="VFH60_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6312221.1"
                     /translation="MNFELTPEQQRIQSLAREFAQEEVAPISREADERGEFPLHLVPR
                     MGELGLLGAIIAPEYSGAELDYLSYALICEELGRADSSVRGFLTVHLGLVAGCIQEWG
                     TEEQKRRYLPRLASGEWIGCYALTEPNAGSDVAGMESTAREDGDGYLLDGEKIWITNG
                     TSANLAIVFASIDREAKHRGICAFLVETGMPGFEREPMPGVELGHRASEHVRITMRDC
                     RVARSAMLGEPGQGFKVAMSALDRGRLGVAAGAVGVGQACLEACVAYTKERRQFGQRI
                     ADFEMVQAALSDMAADVEAARLLVYKAAWTKDRGLPATRATSIAKLFATEAAVRAASE
                     AVLLHGNRGYSNEYPVERYYRDIKGLQIYEGTSHIQRIIIARELVGREKRE"
     gene            complement(3221..>3693)
                     /locus_tag="VFH60_00180"
     CDS             complement(3221..>3693)
                     /locus_tag="VFH60_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312222.1"
                     /translation="ITGRKVITWQQLGSYGMGGPGFFGLELAARGRYPREWLVLTLWG
                     AGGWLLLDGRWLEAHPSQYHIQRPLFSNFGGTEEWDELTGRLAGAKVLEARITDSSSL
                     IVLRQGSQQRILEVPEDTTRLSVWGGSLEQKVWNHAESHLDAWVVSRRGNLFTG"
BASE COUNT          589 a         1206 c         1155 g          743 t
ORIGIN      
        1 ctacagtttg cagacaaacc ctggctgtat actatcacga ttccggccga atcgccctgc
       61 gaatcaggtg cgcccggcct gataacgctg cctgtgctcg ccgggatggg agcgcgcaaa
      121 tgccctttcg cggctatcct ccccgcctcc tcaggagtgt cgtagcaatc ccgccgagcg
      181 ccatgcctcc cagtgccgct aacacagccc atatcagcac gtcattgccg ccctcattgg
      241 gccgtggggg aacggccgtc atcgtggctg tggcctcggg aggggcaccc ggcgctgttg
      301 tagaggctgc tgggcctgtc gggtctgccg gggttgttcg agttgttggg gttgttggta
      361 gtggtgagct tgttgaggct gtcgcgctgg gctggtccac cactcccgtt gcggaggggg
      421 agacggtagg ctcggcggta gacgcaccgg tctgggtggg tgatagggcg gtcggggtgg
      481 gtaccggcag gtcgggcacc agggtagggg tgccgagcgg gagcggccgg tcggtcgagg
      541 cgcgcatctc cttgtacacg tcctgggcgg tcttgttctg cgggtcgagg gcgagggccg
      601 cgcttatctc gttgaggata cgcgctatcc aggggtcgcc ttcctctgga aaagcttgta
      661 ccgccagttg gttccacatg agcgcggcaa actcggcgtg gagttgggcg tcgttgggtt
      721 gcagggcgat agcgtgggcg tacgcctcct ggctgagggc ggcgaacggg tcgttgtcga
      781 agatggggta gcggctcgtg gtcgcggcct ggtatgcctc tgccaggctc acccagacct
      841 gtgggtccat cgggcttacc tccgtcgcgg cacgagcctt gaggacggcc tcccacgttg
      901 caggggcgag cacagtcacc tccaggttgc tctcgtgggt cggctccagg tcgtcccagt
      961 gccaccgaat gaagttgcca tcgaacctgc ctcccggcgt tgtctggcgt tcggagtagg
     1021 ctagcacatt ctcgctcgtg gctgtgtaag gtaggcgaac gctgaggtcc gccgagccaa
     1081 tggtgtcctt ccaacccgcg ccggtctcca ggatataagt aaactgttgg gcagatagat
     1141 tgcctgtacc gtcggtcgta tagcgcacac cgatgcgcac ctcctcacct ggggggaacg
     1201 tggcatcgaa ggttgcccag cggattggcg ctgtttccgt cctaagagga ttttcagtgg
     1261 tgataatacc ggtggtgact actttcccgt ctacgctggc ctgaaaatct cggacttcag
     1321 ggtactgacc aaaaccacta ccttgcccgc ttgggtccgc gagggggaag cgcacctgca
     1381 tctgctcggc tgtatcgctt gtgttcagca tgatgaattc gcagctgacc tcagctacag
     1441 ccttgtcatt gccggccgat atgtcgagca ctacctcctc cgactgcatc ctgacctgcg
     1501 tggtgccctc ggcggggctg atattgctgc cgggtggctg ccggggcggg gcgacgtcgg
     1561 ccaagccagg tgtagcgcct gcggtccagg ccaacagcag ggctaccagg agtcctgccg
     1621 ggcatgccct acgttccggc ttgacgcggt gcaggtgggt cgtcatgtta cggtctccgg
     1681 tcatggttgt tggaaattgt cgaacgggtc gtagggtccg gctgccggtt agggacgtgg
     1741 gaggccggta gcaccacgat agtcactccc gcttctctct gcctaccaac tcccgcgcga
     1801 tgatgattcg ctggatgtgg ctggtgccct cgtagatttg caggcccttt atgtcccggt
     1861 agtatcgctc gaccgggtat tcgttggagt agccccggtt gccgtgcagc agcaccgctt
     1921 cggaggccgc gcgcacggcg gcttcggtgg cgaacagctt ggcgatagag gtggccctgg
     1981 tcgcgggcag gcctcggtcc ttggtccagg cggccttgta caccagcaga cgggcggcct
     2041 ccacgtcggc ggccatatca gagagggcgg cctgcaccat ctcgaagtcc gcgatgcgct
     2101 gcccgaactg ccgccgctcc ttcgtgtacg ccacgcacgc ctcgaggcac gcctgcccca
     2161 cgcccaccgc gcccgccgcc acccccagcc tgccccggtc cagcgccgac atcgccactt
     2221 tgaagccctg gcccggctcc cccagcatgg ccgagcgcgc cacccggcag tcgcgcatgg
     2281 tgatacgcac gtgctcggag gccctgtgcc caagctccac ccccggcata ggctccctct
     2341 cgaagccggg catacccgtt tccaccagga aagcgcagat gccccggtgc ttcgcctccc
     2401 tgtcgatgct ggcaaatacg atggctaaat ttgcgctggt gccgttggtg atccatatct
     2461 tctcgccgtc gagcagatag ccgtcgccgt cctcccgcgc ggtggactcc atacccgcca
     2521 cgtccgaccc cgcgttcggc tcggtgaggg cgtagcagcc tatccactcg cccgaggcca
     2581 gcctggggag gtagcgtcgc ttctgctcct ccgtgcccca ctcctgtatg cagcccgcga
     2641 ctagtcccaa gtgcaccgtg aggaaacccc gcaccgacga atcggcccgc ccaagctcct
     2701 cgcagatgag cgcgtaactg aggtagtcca gctccgcccc gctgtactcc ggcgcgatta
     2761 tggctcccag caagccaagc tcgcccatgc gcggcacgag atgcaggggg aactcgcccc
     2821 gctcgtcggc ctcccgcgag atgggggcta cctcctcctg cgcgaactcg cgggcgaggg
     2881 actgtatgcg ctgctgttcg ggtgttagct cgaagttcat agagtgcctg ttgctggatt
     2941 cataagcgtt tccggtggtt tatgctatct gctaaggagt atctgcaaat ttattagata
     3001 ctggcagcca aaggtggccc cttgtcattc tgagcgtagc gaagaatcag ccacccgtgc
     3061 ttgatactcc caacgcgggg taccattttc gcctttacca ccccgctccc atgcgctcaa
     3121 caatctagct acccacctga ttcttcgctc cgctcagaat gacaaagtgg agtatggtcc
     3181 cgcacacgca gcgctttgcg gatacgcccc tcgatactca tcagcccgta aacaggtttc
     3241 cccgccgaga cacgacccag gcatccaggt ggctctcggc atggttccac accttctgct
     3301 caagcgaccc accccacacg gaaaggcgcg tcgtgtcttc cggcacctct aatatgcgct
     3361 gttggctgcc ttgccgcagg acaatcaggg acgaggagtc ggtgatacgc gcctcgagga
     3421 ccttcgcgcc cgccagccta cccgtcagtt cgtcccactc ctcggtgccg ccgaagttgc
     3481 tgaacagggg gcgttggatg tggtactggc tcgggtgggc ttcgagccac cggccgtcga
     3541 gcaacagcca cccgcccgct ccccacaatg ttagcaccag ccattccctc ggataacgcc
     3601 ctcgcgccgc caactccagc ccgaagaagc ccggcccgcc catgccgtag ctgccgagtt
     3661 gctgccacgt aatcaccttg cgcccggtga tgc
//
LOCUS       DASRWZ010000015         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_343732, whole genome shotgun sequence.
ACCESSION   DASRWZ010000015 DASRWZ010000000
VERSION     DASRWZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343732"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(487..945)
                     /locus_tag="VFH60_00185"
     CDS             complement(487..945)
                     /locus_tag="VFH60_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6312223.1"
                     /translation="MTPTSTQHIVFTLDSQRYALPLTDVEWVVQPTRLTLLTGSQGAV
                     MGLVNVHGWKIPVVSLRKLHGKAERDLAPGNRLIILRRHDRKVALLVDSVLDGVTCDE
                     GTRAPAPHAAHYPDTPETEDLVLLYEASRLLDSGAYHAGAAPNRIGRVMI"
     gene            complement(1239..1484)
                     /locus_tag="VFH60_00190"
     CDS             complement(1239..1484)
                     /locus_tag="VFH60_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312224.1"
                     /translation="MVVVAPIAAEGVRGTTVPATVARGVAVPAAVLLEGVALLGLCLR
                     EAGPQPPATKTRDAHRTITQGCFLMMLLRMEGRRGYE"
     gene            1504..2823
                     /locus_tag="VFH60_00195"
     CDS             1504..2823
                     /locus_tag="VFH60_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HET6312225.1"
                     /translation="MNGDLDTVRLAAHPVEGEAGDYDPLMSLVGDARFVLLGEASHGT
                     HDFYRERARITRRLIEDKGFTAIAVEGEWQRAYRVNRYIRGFSDEPDAGAALSGFVGF
                     PEWMWNNTDVRDLAEWLKKHNAAVPRDASQVGFYGLDMYGIKESADAVAEYFDERDPT
                     AARQVRDRYSCLSRTGFDPADYESPADTPNASCNTRIEEQIAQLQEMRERAEREGYGG
                     NSAVEREELFSALRHAVVVKSKREYDRSISTGPDSSWNVRDRHMASTLEALSAHLGAA
                     GEPAKVVVWAHNTHVGDARATEMTERQELNLGQLARERYKEDAVLVGFTTYTGTVIAA
                     RSWDEPGERYDVRPALPESYSGLFHDAGMGNSLFILRGDERLAEALDEPRLQRGIGVV
                     YLPETERFSHYYQAWLSRQFDAVIHIEESSALTPLEPAPGTPHTNSP"
     gene            complement(2926..>4007)
                     /locus_tag="VFH60_00200"
     CDS             complement(2926..>4007)
                     /locus_tag="VFH60_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET6312226.1"
                     /translation="PPTATTLQPTQTIAAQPTVPPSPTAEPPTPTAPAASTPTAPPLS
                     PGGWALVTDSEDGPAPAPRYDHSLVADPERHQILLFGGRGSGTFGDTWLFDLAGEEWR
                     EVQGDGPPARFGAGAVYDGANRRVLLVMGQGDSGFFNDVWAFDLDEEAWTQLKANTVA
                     DDEPRPRYGQSAALHKDGRVLISHGFSDQGRFDDTWAFDPNTAKWVNITPTSGPIPLK
                     RCLHELAYDPRDDLLLMFGGCSSGYGPCPQGDLWALDLASGTWTELQPQGEKPSPRSN
                     PSLAYDPDRRAIFLFGGKAQDGPVSDTWVYMLDGNGWGTVESEISPSPRSSQGTAYDP
                     TRRRVYIAGGDTKEGPDMEVWVSGF"
BASE COUNT          693 a         1243 c         1345 g          726 t
ORIGIN      
        1 tcacggcttc cacgaccgcg aacgccccgc ccgtctgctc gctccgcagc ttgatggtca
       61 taccggggcc aatagcctca cccccgcccg gtgggagcac tacttcacgc agacctgccg
      121 gggatatttc ttcgacaacc atgattttcc tccttcgtac agtggtcgct tgcttgtgat
      181 ttgattaaag caaacaaccc gtgccaacgg tgagcacaag aactcgcaca gtatttcgca
      241 cacccgcaca atgagtagta cacgaccgca aatgggccag gagagcgcca gggagataaa
      301 gattattcac cgcagagacg cagaggttac gcagagacac gtgttggttg gaaatagaca
      361 agggcagcat cgcacagtac gatgctgccc ttgtctggac accgtttatt cttctctgcg
      421 tgcctcagcg tgctccgcgt ctctgcggtg aaatcgttct gacggttgag gtgcggccca
      481 ccggggttag atcatgacgc ggcctatcct gttcggcgcg gccccggcgt ggtacgcgcc
      541 cgagtctagc aggcgggagg cttcgtagag cagcactagg tcctcggttt cgggggtgtc
      601 gggatagtgc gcggcgtggg gtgccggtgc tctcgtgccc tcgtcgcagg tgacaccatc
      661 cagcaccgaa tcgaccagca gggccacttt ccggtcgtgc ctgcgcaaga tgatgagcct
      721 gttgccgggg gcgaggtcgc gctccgcctt accgtgcagc ttgcgcaggc tcactaccgg
      781 tattttccag ccgtggacgt tgaccaagcc catgaccgcg ccctgcgacc cggttagaag
      841 cgttagcctg gttggctgca ccacccactc cacatccgtg aggggcagag cgtaacgctg
      901 gctgtccagg gtaaagacga tgtgctgcgt tgacgtgggt gtcatagtcg ttcctccaac
      961 gcgacgggcc gtggtgcatg aagcgcctcc tgcaaccggc atgcgtcgtg tgcccatgta
     1021 tagggcttgt gaccattatg gggaatagag aggccggtct gaatgtctca ttttcccata
     1081 attataggga cttacgggca gatttcatgg gacttgcccg ccctgcgagt ggtgtgctgt
     1141 gtcgcccggc agtgagaacg ccagaagggg ggcttggctg cccgtagatg gcggtcttat
     1201 tctttgggat gacttcaacc acctggcttc atatttgctt actcatagcc tctgcgtcct
     1261 tccatgcgga gcagcatcat gagaaagcaa ccctgggtaa tagtcctgtg tgcgtccctg
     1321 gtcttggtcg caggcggttg cggccccgcc tcgcgcaggc ataaccctag cagcgcaacc
     1381 ccctcaagca gcacagcggc gggcacggcc accccccgcg ccaccgttgc cggcactgtt
     1441 gtacccctga cgccctcggc ggcgataggt gcaaccacta ccactccatt gcccggtgcc
     1501 tccttgaatg gcgatcttga tacggtccgc cttgccgcgc atcccgtgga gggcgaagct
     1561 ggggattacg acccgctgat gtcgcttgta ggcgacgccc ggttcgtgct gctgggggag
     1621 gccagccacg gcacgcacga cttctaccgg gagcgggcgc gcatcacccg caggttgata
     1681 gaggacaaag gttttacggc gattgccgtg gagggcgagt ggcagcgggc ctaccgtgtg
     1741 aaccgctaca tacgcggctt cagcgacgag ccggacgccg gggcggccct gtcgggcttc
     1801 gtgggcttcc cggagtggat gtggaacaac accgacgtgc gcgacctggc ggagtggctc
     1861 aagaagcaca acgcagctgt gcctcgcgac gcctcgcagg tcgggttcta tggcctcgac
     1921 atgtacggca tcaaggaatc ggcggacgcg gtggccgagt actttgacga gcgagacccc
     1981 acggccgcca ggcaggtgcg cgaccgctat agctgcctct cgcgcaccgg gttcgacccg
     2041 gcggactacg aatcgcccgc cgatacgccc aacgcttcgt gcaacacccg catcgaagag
     2101 caaattgcgc agttgcagga gatgcgggag cgtgcagagc gcgaaggcta cgggggcaat
     2161 agcgcggttg agcgcgaaga attgttctcc gccctgcgac acgccgtggt cgtgaagagc
     2221 aagcgcgagt acgaccggtc gatctccacc ggccccgact cgtcgtggaa cgtgcgggac
     2281 cggcacatgg cgagcacgtt ggaagccctg tcggcccacc tgggcgcggc tggggagcct
     2341 gctaaagtgg ttgtgtgggc gcacaacacg cacgtggggg atgcccgcgc caccgagatg
     2401 acggagcgcc aggagctaaa cctggggcag ttggcgcggg agcggtacaa ggaggacgct
     2461 gtgctggtgg gcttcaccac ctatacgggc acggtgatag ccgcccgaag ctgggacgag
     2521 cctggggagc ggtacgacgt gcggcccgcc ctgcccgaaa gctactccgg cctgttccac
     2581 gacgcgggga tgggcaactc tctcttcatc ctgcgcggcg acgagcggct ggccgaagcc
     2641 ctggacgagc cgcgcttgca gcggggcatc ggcgtcgtct atctgcccga aaccgagcgt
     2701 ttcagccact actaccaggc ctggctctcc cggcagttcg acgcggtgat tcacatcgag
     2761 gagtcgagcg ccctcacgcc gctggagcct gcgcccggca ctcctcatac caattcacct
     2821 tgaaacaact gaaacagggg aaagggggga accggggaca tcccagacct cgacaggagc
     2881 gcacccctgc accccctcag cgcgggccat cggatcagac ctgtcctaga acccgctgac
     2941 ccacacttcc atgtcggggc cttctttcgt atcaccgccc gctatgtaga ccctgcgccg
     3001 ggttgggtcg tatgccgtgc cctggctgct gcgcgggctg gggctgatct cgctttcgac
     3061 ggtcccccag ccattgccgt ccagcatgta aacccaggta tcggacacgg ggccgtcctg
     3121 ggccttgccg ccgaacagga agatagcgcg ccggtctggg tcgtaggcga gggaggggtt
     3181 gctgcgcggg gagggttttt caccctgcgg ctgtagctcg gtccaggtgc cggaagcgag
     3241 gtcgagcgcc cagaggtcgc cctgggggca agggccgtag ccggaggagc agccgccgaa
     3301 catgaggagc agatcgtctc gtgggtcgta ggccagctcg tgcaggcagc gcttgagggg
     3361 gatagggccg ctcgtggggg taatattgac ccatttggct gtattggggt cgaacgccca
     3421 ggtatcgtcg aagcggccct ggtcggagaa gccgtgggag attagcaccc ggccgtcctt
     3481 gtgcagggcc gccgactggc cgtagcgcgg tctcggctcg tcgtccgcga ccgtgttggc
     3541 ctttagctgc gtccacgcct cctcgtccag gtcgaaggcc cacacgtcgt tgaagaagcc
     3601 gctatcgccc tggcccatca cgagcagcac gcggcggttg gccccgtcgt acacggcacc
     3661 ggccccgaag cgcgcgggcg ggccatcacc ctgcacctcg cgccactcct cccctgctag
     3721 gtcgaacagc catgtatcgc cgaaggtgcc gctaccacgc ccgccgaaca acaggatctg
     3781 gtggcgctcc gggtcggcca cgagcgaatg gtcgtagcgg ggtgccggag ccggtccgtc
     3841 ctccgaatcg gtgaccaagg cccacccgcc gggggacagg ggtggtgcgg taggcgtgct
     3901 tgctgcgggg gcggtggggg tcggtggttc ggcagtcggg ctgggtggta cggtaggttg
     3961 ggcggcaatg gtctgtgtag gttgcagggt ggtcgctgtg ggcgggg
//
LOCUS       DASRWZ010000016         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_138118, whole genome shotgun sequence.
ACCESSION   DASRWZ010000016 DASRWZ010000000
VERSION     DASRWZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138118"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..847)
                     /locus_tag="VFH60_00205"
     CDS             complement(155..847)
                     /locus_tag="VFH60_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HET6312227.1"
                     /translation="MSQQDQGKVLYSPLFVAVSGLFVACLIAANIISVKLIAVGGWFV
                     PAGVVIFPLSYVFGDVLTEVYGYRSARRVIWLGFLCNLVVVGAIWVAGAWPAAPFWQD
                     QPAWEAILGFAPRLLVASFVAYLVGEFANSFILARMKVATQGRWLWSRTIGSTVVGEG
                     LDSLVFITIAFAGVPGIDLTTAILTQWTIKVAYEVLATPFTYLVVGYLKRVEGIDVYD
                     RNTSFNPLALAD"
     gene            complement(1170..2285)
                     /gene="ychF"
                     /locus_tag="VFH60_00210"
     CDS             complement(1170..2285)
                     /gene="ychF"
                     /locus_tag="VFH60_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET6312228.1"
                     /translation="MALNLGIVGLPKSGKTTLFNAITGSHVATSAYASGEQPNVAVVK
                     VPDERLDVLAGMFHPRKLTPADVQFTDVVGMAAGKDTGNKEPISRQVLGFISTVDALV
                     LVVRAFENAAVPLPEGTEGVDPLRDAESVMLELAFSDLGIIERRLERLKGEIQKTKGP
                     ERELREREQVVLTRIGPALEEGKHIRDLGLNEDDEKVIRNFGFLTAKPMLIVANLGED
                     NLDSAPEIEARISKQWSSASTQVVAIPAQLEMEISQLSPEDAAEFRESLGMGPSRLGE
                     VVARSYTLLGLISFLTAGEDEVRAWTIRRGTVAQRAAGVIHSDLERGFIRAEVVHYDD
                     LIAAGNMVEAKKRGVLRSEGKTYIVKDGDIVNVLFNV"
BASE COUNT          469 a          798 c          732 g          484 t
ORIGIN      
        1 gtgctgttgg acgagattct tcgctgcgct cagaatgaca aagtggggac ttgggtgctc
       61 ctgttcgaca atattgcttt gagaacttct agtgcgtggt cagcgttcgt tctgcccgga
      121 cccggcaagc caatactctg ataagagtgc cgcgtcaatc agctagggca aggggattga
      181 agctggtgtt gcggtcgtac acatctattc cttcaacgcg cttgaggtag cccaccacca
      241 ggtaagtaaa gggggtggct agcacctcgt aagctacctt gatggtccat tgggtgagga
      301 tggcggtggt aaggtcgatg cccggcacgc ccgcgaaggc gatagtgatg aacaccagcg
      361 agtcgaggcc ctcgcccact accgtcgagc cgatggtgcg gctccaaagc caccgtccct
      421 gcgtggcgac cttcatccgc gccaggatga acgagttggc gaactcaccc accaggtagg
      481 ccacgaacga agccaccagc aagcgcgggg cgaaccccag gatggcttcc caggcgggtt
      541 ggtcctgcca gaacggcgcg gcgggccagg ccccggccac ccatatcgcg cccaccacga
      601 ccaggttgca caggaaaccc agccagataa cccgccgcgc gctccggtag ccgtacacct
      661 cggtgagtac gtcgccaaag acgtagctga ggggaaatat caccacaccg gcgggcacga
      721 accagccccc cacggcgatt agcttcaccg atatgatatt tgccgctatc aggcaagcca
      781 cgaacagccc gctcactgcc acaaagagcg gcgaataaag cacctttccc tggtcctgct
      841 ggctcacttc gactcctggt attgcctgct atttcccctt ttgcttgtaa acacgggcag
      901 attataacat tcctgacggc tgagtggcac caaacccgtg ccactcaaag gcaagcagag
      961 ccggtgcaaa ggaagaagct gaattgaagg tcagcattgg ccttgcttcc tttgctcctt
     1021 ccgatgcaac aaagaacgtc tgccaactga ttcctcactc cgttcggaat gacagagggg
     1081 aagcacggtc ctgttgattg tggcaccacg aatcttgcac tcgtcttgtg cctatctcag
     1141 accatgttat gcgggtgccg ggcgcttcct tagacgttga acagcacgtt cacgatgtcg
     1201 ccgtccttga cgatgtaagt cttgccctcg ctgcgcagca cgccccgctt cttggcttcc
     1261 accatgttgc ccgccgcgat gaggtcgtcg tagtggacta cttcggcgcg gatgaagccc
     1321 cgctctaggt cggagtgaat caccccggcg gcgcgctgag ccactgtgcc ccggcggatc
     1381 gtccaggccc gcacctcgtc ctccccggcg gtgaggaagc taatcaggcc caacagcgta
     1441 taggagcgag ccactacctc gcccagccgc gacggcccca tgcccaggct ctcgcggaac
     1501 tcggcggcgt cctccggcga aagctgcgat atctccatct ccagctgcgc cgggatggcg
     1561 accacctggg tggacgcgct gctccactgc ttcgagatgc gcgcctcgat ttcgggggcg
     1621 ctatcgaggt tgtcctcgcc caggttggcc acgatgagca tcggcttggc ggtcaggaag
     1681 ccgaagttgc ggataacctt ctcatcgtcc tcgttgaggc ccaggtcgcg gatgtgcttg
     1741 ccttcttcga gggcggggcc gatgcgggtc agcaccacct gctcgcgctc tcgcagctcg
     1801 cgctccggcc ccttcgtctt ctggatttcg cccttcagcc gctccaaccg gcgctcgatg
     1861 atgcccaggt ctgagaatgc aagctccagc atcacgcttt cggcatcgcg cagcggatcg
     1921 accccctcgg tgccctccgg cagcggcacc gccgcgtttt cgaaggcccg caccaccagc
     1981 acgagcgcgt ccaccgtgct gatgaagccg agcacctgcc tgctgatagg ctccttgttg
     2041 ccggtgtctt tgccagccgc catgcccaca acgtccgtga actgcacgtc ggcgggggtg
     2101 agcttgcgcg ggtggaacat gccggccagc acgtccagcc gctcgtccgg caccttgact
     2161 accgccacgt tcggctgctc gccgctcgcg taagcggagg tggcgacgtg gctgcccgtt
     2221 atcgcgttaa atagggtcgt cttgccgctt ttcggcagtc cgactatacc caggttcaaa
     2281 gccattcagt agcgctccat ctgtgtcagt aatgcaccat tgtaccgcat ggggtcgaat
     2341 aggggccact tcaggcagga ggtcagcagg ggcatgtcac agcaggagag acagaatcaa
     2401 gcctgtccgg catcctgtcg gaaggtggca cccaggagtg gctcttgtca tcctgagcgt
     2461 agcgaaggac cagccgcccg tgt
//
LOCUS       DASRWZ010000017         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_754854, whole genome shotgun sequence.
ACCESSION   DASRWZ010000017 DASRWZ010000000
VERSION     DASRWZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754854"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VFH60_00215"
     CDS             <1..1494
                     /locus_tag="VFH60_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6312229.1"
                     /translation="GKLDEAALTGGPLSPVVARGVLPTSMRLHVDARYQGRGVTLALV
                     DSGFYQHPELTLPNNRIRAWVNATTDPVSALFFDPDDRPEWPGWNSASPSQWHGTMTS
                     GVAAGNGCLSHGLYCGLAPGAQVVLVQARGKDGCISNETITRALRWLEERGPELGVRI
                     VSLSVAGEPCDWLAGNPVDEAIAALVQQGVTVVTAAGNSGVRELVPPATAPDALTIGG
                     IDDHNNFDPQDVELWHSNYGATVSGRIKPELVAPSIWVVAPVLPGSQVAEEAQELFGR
                     RHLHEPGTEARITELKLITPHYQHVDGTSFAAPIVASVVACLLEANPDLTPRQIREIL
                     TSTAQPIEGVSRQRQGAGALNPTRALAAAVRVEGAPMSGYPMSPHVSPYGVTFYLYDR
                     HTNSVQVQGSWDDWASPGVEAEQVLPNIWRAHVDNLQPGRYTYRFLLGGVYWLDDPDN
                     PAKTPNTFGGFDSVLEVPEQPADPSVGAALETKTQRRKGRKERAKSS"
BASE COUNT          269 a          532 c          488 g          217 t
ORIGIN      
        1 ggtaagctgg acgaggcagc cctcaccggt gggcctctgt cacccgtggt ggcgcgtggg
       61 gtgctgccga cctccatgcg cctgcacgta gacgcgcgct accagggcag gggcgtcacg
      121 ctggcgctgg tagactccgg cttctaccag caccccgagc tgaccctgcc caacaaccgc
      181 atacgggcct gggtgaacgc caccaccgac ccggtcagcg ccctcttttt cgacccggac
      241 gaccgccccg agtggcccgg ctggaacagc gcctctccct cgcagtggca cggcacgatg
      301 accagcgggg tagcggccgg caacggctgc ctgagccacg gcctctactg tgggttggcc
      361 cccggagcgc aggtcgtgct tgtgcaggca cgcggcaagg acggctgcat cagcaacgag
      421 acgatcaccc gcgccctgcg ctggctcgaa gaacgcggcc ccgaactggg cgtgcgcatc
      481 gtcagcctct ccgtcgcggg cgagccgtgc gactggctgg cgggcaaccc cgtggacgag
      541 gcgattgccg cgcttgtgca gcagggcgtc accgtcgtga ccgcagccgg caactccggc
      601 gtgcgcgagc ttgtgccccc ggccaccgcc cccgacgccc tcacgattgg cggcatcgac
      661 gaccacaaca acttcgaccc gcaggacgtc gaattgtggc acagcaacta cggcgcgacg
      721 gtttcgggcc gcattaagcc ggagcttgta gccccgagca tctgggtggt ggcccccgtg
      781 ctgcccggct cccaggtggc cgaggaggcg caggagctat tcggcagacg gcacttacac
      841 gaacccggta ccgaggcccg catcaccgag cttaagctga tcaccccgca ttaccagcac
      901 gtagacggca ccagctttgc cgcgcccatc gtggctagcg tggtggcctg cctgctggaa
      961 gccaacccag acctcactcc caggcaaata cgcgaaatac tcacctccac ggcgcagccc
     1021 attgaagggg tgagccgcca gcggcaaggg gcaggggcgc tcaaccctac gcgggctttg
     1081 gcggcggccg tgcgcgtcga gggcgcgccg atgtcgggtt accccatgtc gccgcacgtg
     1141 agtccctacg gggtcacgtt ctacctctac gaccggcata ccaactcggt acaggtgcag
     1201 ggaagctggg acgactgggc ctcccctggc gtcgaggccg agcaggtgct gcccaacatc
     1261 tggcgggcgc acgtagacaa cctccagccg ggccgctaca cctaccgctt cctcctcggc
     1321 ggcgtctact ggctggacga ccccgacaac cccgctaaga cccccaacac gttcggcggc
     1381 ttcgacagcg tgctcgaagt gcctgagcaa ccggcagacc cctctgtcgg ggcagctttg
     1441 gagacgaaaa cgcaaaggcg caaaggacgc aaagaacgcg caaagagtag ctgaggttta
     1501 gagggt
//
LOCUS       DASRWZ010000018         6711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_892131, whole genome shotgun sequence.
ACCESSION   DASRWZ010000018 DASRWZ010000000
VERSION     DASRWZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892131"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFH60_00220"
     CDS             complement(<1..222)
                     /locus_tag="VFH60_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312230.1"
                     /translation="MPQLSPRQKGIFFVLLAAGLWGTLGTFYRLGTDEFHLTPRAIVF
                     WRGAVAAIALGVVMGVILPIGAGKRGLLRL"
     gene            complement(392..2674)
                     /locus_tag="VFH60_00225"
     CDS             complement(392..2674)
                     /locus_tag="VFH60_00225"
                     /inference="COORDINATES: protein motif:HMM:NF022812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HET6312231.1"
                     /translation="MNSHIKNRLLALAMSAIVLVAAAGCDLNTPTPTPVANPTGTATS
                     PQPTATVGTTGGATAEPTPESTPQGTPAAGVTPSVRHAVRFAHYNLQPSNVTPAVAPY
                     TVAPDLSNVSVAEAFGLSDEMKGLIAKYGFAAQFPKEFSHKQFYQLYEDGRYDTESVF
                     VTTDSVLHVYHLIFDKILRSTETKYLISDLEDLTAGLLKASQDQYDQLKGTEGEQAAR
                     RNVAYFTVASRLLDANAPVPDYVRTEVAQELQQIDRHEGLTPSAVMAIGNPEMFVEDY
                     GQYKPRGHYTRSEDLSRYFRAMMWYGRITFRLKLVEETRSALLLTRALQTAQAGDTTA
                     EAAWARIYEPTAFFVGGADDLTFRDYAPVIEQAIGANASLEDLVSTANVEKFQEAAKS
                     LAGPRINSMFVYVTEDKETVTKGLRMMGQRFTLDAYVFGQLIWRNVGSQENPRTLPKA
                     LDVPAAFGSEEAYEILEDMGETKYEKFDRQLSRVRGEIASLDTSQWTENLYWSWLYTF
                     RPLLEPKSPDSGYPSFMTNEAWTRKNLNTVLGSYTELKHDTILYAKQVMAEAGGGPPE
                     IVKGYVEPEPEFYARIAALSAMTRDGLLSRGLLEKTEDRSAISDFNTLNQIESLALDL
                     KRISEKELENKALTQEEYELIRFYGARIEHLTIAAADQEEGQGGPSLQDQDAAVVADI
                     ATGGPQLDQALEEATGRIMEIYVVVPIAGNLVLARGGIYSQYEFVQPSSNRLTNEQWR
                     QRLDNGQVPEEADWKVFIGK"
     gene            complement(3022..4728)
                     /locus_tag="VFH60_00230"
     CDS             complement(3022..4728)
                     /locus_tag="VFH60_00230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET6312232.1"
                     /translation="MSKIAISISLVVALLAGAFAQVGGARTAGGNSAPSNDEVSLVFV
                     GDLMLGRYVGSTLAARGYDTPFEGVRALLQPADLAVGNLESPLVRRGSIRVPPPSPNQ
                     LNLTGDDRAAPALSRAGFDLLSLSNNHALDSGAFGLQSTVNALRASSLVPFGLADKQG
                     GQLPVIRDVRGVKVAFLGYTTILNIITNDMRSNGTLRPGIGYVNPTSKADKDLFAQQI
                     ASARTKADVVVVFMHWGNEYKTQPDSWVVDLGKLAARSGADLVVGAHPHVAQGMKVEL
                     EGTGGRSTVVAYSLGNALFDQMARMETRQGLALSVRADRHGVKSARLVPLEITAGSYK
                     MRVADDASGQPTAKRAALSTPPDLQWRAIWDANQPQPGVAIGYRRVAQSQERASVEDL
                     GLGAPTRVELLNGLLTVQSGDGKGNWKTVWQTEPTWRVTGYTVGDANADGKPDLVYSL
                     WKKRLTWERPDDGGLQVDMEGGDFLPHIYINSWRDGALMPLWHGSPRPSPILDLAVVP
                     LGPAGKPTLGVFDSADPAKEQPPGKVNLWHWTGSFGFELLKSLPGTYSTTWGEGKLLL
                     LK"
     gene            complement(5317..6555)
                     /locus_tag="VFH60_00235"
     CDS             complement(5317..6555)
                     /locus_tag="VFH60_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET6312233.1"
                     /translation="MGHDMDKKDKRDGQQRLLSTLEQLLSIEATNLKSALDQASDLIV
                     GAIGADKADAFILDPTKETLVAEGTSHTPMGVRQHQIGMHLLPLANDGPEAAVFFTGE
                     FYNNGHAESDPTMPPGMTKGLGIRSMMIVPLVVNGERRGVLQACSSKQDAFSEDDLSF
                     LHAVSHWVGALAHRAELVEHIAQDAAAQARQVVAEELVTVLAHDLRNYITPLKGRLSI
                     ILDRAHREDRPRDIDDADAALRAVRRLQALITDLLDVARLDQGVFSLSREPLNLVTLV
                     EEATGVMQTARLDILLRLPDELRVEADPARLKQALENLIANALRHSPKGVPIDVGLAS
                     ERREDGEWAVLTIRDEGSGIAPELMPRLFTRFASGPDSTGLGLGLYLARSIAEAHGGT
                     LTVTSSPGQGTSFQLALPAL"
BASE COUNT         1186 a         1961 c         2233 g         1331 t
ORIGIN      
        1 caggcgcagc aacccccgct tgcccgcacc gatgggtaag atgacgccca tgaccacacc
       61 cagggcgata gccgccaccg caccccgcca gaacacgatg gcacgcggcg tcaggtgaaa
      121 ctcgtcggtg ccgagccggt agaacgtgcc cagcgtgccc cacagaccgg cggccagcag
      181 cacgaagaag ataccttttt gcctggggga aagctgaggc atgaagaaat ttggaaattg
      241 gaatttcgaa tttcggattt ggggtgaaga ttaacacgta aagcgtaaca cgtgagggag
      301 taagatacaa caagagcggc tcacatggag catagtccat agagccgcct tgcttattac
      361 gagttacgct ttacgtgtta ctcttcactc gtcacttgcc gatgaagacc ttccagtcgg
      421 cttcctccgg cacctggccg ttgtcgaggc gctggcgcca ctgctcgttg gtgaggcggt
      481 tgctggaagg ctggacgaac tcgtactggc tgtagatgcc accgcgtgca aggacaagat
      541 tgcctgcgat gggcaccacc acgtagatct ccatgatgcg accggtcgcc tcttcaaggg
      601 cctggtccag ttgcgggccg ccggtggcta tgtcggctac gaccgcggcg tcctggtcct
      661 gcaggctggg gccaccctgg ccttcttcct ggtccgcggc ggcaatcgtc aggtgctcga
      721 tgcgagcgcc atagaagcga ataagctcgt actcttcctg ggtgagcgct ttgttctcca
      781 actccttctc gctgatacgc ttcaggtcga gggcgaggct ctcaatctgg ttgagggtgt
      841 tgaagtcgga aatagcggac cggtcctcag tcttttcgag caggccgcga gacagcaggc
      901 catcgcgggt catggcagag agggcggcga tgcgggcgta gaactcgggc tccggctcca
      961 cgtagccctt gactatctcc ggcggaccgc ccccggcctc ggccatcacc tgcttggcgt
     1021 agaggatggt gtcgtgcttt agctcggtgt agctgcccag gaccgtgttc aggttcttgc
     1081 gggtccaggc ctcgttggtc atgaagctgg ggtagccgct gtcaggtgac tttggttcga
     1141 ggagcgggcg gaaggtgtag agccaggacc agtagaggtt ctcggtccac tgggaggtgt
     1201 ccagactggc gatttcgccc cgcaccctgc tcaactgccg gtcgaacttc tcatatttgg
     1261 tctcgcccat gtcttccagg atctcgtatg cctcctcgga accgaaggcg gcgggcacgt
     1321 ccagcgcctt gggcaacgtt cgagggttct cctggctgcc cacgttgcgc cagataagct
     1381 gcccgaacac gtaagcgtcg agcgtaaacc gctggcccat catgcgcagg cccttggtaa
     1441 cggtttcctt gtcctcggtg acgtacacga acatcgagtt gatccgcggc ccggccagcg
     1501 atttggcggc ttcctggaac ttctccacgt tggcggtgga tacgaggtct tccaggctgg
     1561 cgttcgcacc tatagcctgc tctatcacgg gtgcgtaatc gcggaaggtg aggtcgtcgg
     1621 ccccgcccac gaagaaggcg gtcggctcgt agatgcgcgc ccaggccgcc tcagcggtgg
     1681 tgtccccggc ctgcgcggtc tgtagcgccc gagtaaggag caaggccgac cgggtctcct
     1741 ccaccaactt gaggcggaag gtaatgcggc cgtaccacat catagcgcgg aagtagcgcg
     1801 acaggtcctc ggagcgggtg taatggccgc gcggcttgta ctggccgtag tcctcgacaa
     1861 acatctccgg gttgccgata gccatgaccg ccgatggggt aaggccctcg tgccggtcta
     1921 tctgctgtag ctcctgggcc acctctgtcc gcacatagtc gggcacgggg gcgttagcgt
     1981 cgagcaggcg ggaggccacg gtgaagtagg ccacgttgcg gcgggcggcc tgctcgccct
     2041 cggtgccctt tagctggtcg tactggtcct ggctcgcctt gagcaggcca gccgtcagat
     2101 cctccaggtc ggagatgagg tactttgtct cggtggagcg cagtatcttg tcgaagatga
     2161 ggtggtacac gtgcagcacc gagtcggtgg tcacgaacac tgactcggta tcgtagcggc
     2221 cgtcctcgta tagctggtag aactgcttgt ggctgaattc tttggggaac tgcgcggcga
     2281 aaccgtactt cgcgataagg cccttcatct cgtccgacag cccgaatgcc tcggcgacgc
     2341 tcacgttgga caggtctggt gccaccgtgt agggcgcgac cgccggggta acgttggaag
     2401 gctggaggtt gtaatgggcg aagcgcacgg catggcgcac ggagggggtt actcccgccg
     2461 cgggcgtgcc ttgtggcgtg ctttcggggg tgggttcagc ggtggccccg cccgtggtcc
     2521 cgacggtggc cgtgggttgc ggtgaggtcg ccgtgccggt ggggttggcg acaggcgtgg
     2581 gcgtgggcgt gttgaggtcg cagcctgccg ccgcgaccag cacaatggca ctcatagcga
     2641 gggcgaggag gcgattctta atatggctgt tcatacgctt ttctccttct gtcattttgc
     2701 gttgctgatt gtcatctcga tagttgaata cttatctcta ctgtccagac ctgtcttacc
     2761 cagtgctaga aagcgcaagc ggcgtaacca caggacccta ttcccccttt gtcatcctga
     2821 gcgtagcgac ggatctaaga tgccggcatt atccctacgg tgaacctcca gcacctttaa
     2881 tggtacagtc caacagcatc cctgcccaga ttctttagat ccttcgctac gctcaggatg
     2941 acagggacca ctttggtcct cctacacaac caacttgcaa tatgtaccac tcgttactca
     3001 gtcctcagtc ctccgtcctc attacttcag gaggagcaac ttgccctctc cccaggtggt
     3061 ggagtaggtg ccggggagag attttagaag ctcgaaaccg aagctgccgg tccagtgcca
     3121 caggtttacc ttgccagggg gctgttcctt cgcgggatcg gcgctgtcga acacgcctag
     3181 cgtgggctta ccggcggggc cgaggggcac caccgcgagg tcgagtatgg gtgaggggcg
     3241 aggcgagccg tgccacaggg gcatgagcgc accgtcgcgc caactgttga tgtagatgtg
     3301 gggcaggaag tcgccgccct ccatgtccac ctgcaagccg ccgtcgtcgg gacgctccca
     3361 ggtgaggcgt ttcttccaga gcgagtaaac caggtcgggc ttgccgtcgg cgttggcgtc
     3421 gcccaccgtg tagccggtga cgcgccaggt aggctcggtc tgccagaccg tcttccagtt
     3481 gcccttacca tcgccggact gcacggtaag gaggccgttg agcagctcga cgcgggtggg
     3541 cgcgccgagg cccaaatcct cgaccgaggc gcgttcctgc gactgcgcta cgcgccggta
     3601 gccgattgca acgccgggct ggggctggtt ggcgtcccag atggcccgcc actgcaaatc
     3661 ggggggcgtg ctcagggcgg cgcgcttcgc ggtgggctgg cccgaagcgt cgtcggccac
     3721 tcgcatcttg tagctgccgg cggttatctc cagcggcacc aggcgggcgc tcttgacgcc
     3781 gtggcggtcg gcgcgcaccg agagggcgag gccctggcgc gtctccatgc gggccatctg
     3841 atcgaacagg gcgttgccga gcgaataagc caccacggtg gaacgaccgc cggtgccctc
     3901 aagctccact ttcatgccct gcgcgacgtg cgggtgagcg ccaaccacga ggtcggcacc
     3961 ggagcgggcg gcgagcttgc ccaggtccac cacccagcta tcgggctgcg tcttgtactc
     4021 gttgccccag tgcatgaaca cgacaaccac gtcagctttg gtccgggccg aggcgatttg
     4081 ctgggcgaag aggtctttgt cggccttgct ggtggggttg acgtagccga taccggggcg
     4141 aagggtgccg ttgctccgca tgtcgttggt gatgatgttg agtatggtag tgtatcccag
     4201 gaaggccact ttcaccccgc gcacgtcccg gattacgggc agttggcccc cctgtttgtc
     4261 ggcaaggccg aagggcacca ggctcgaggc gcggagggcg ttcacggtgg attgcagccc
     4321 gaacgcgccg gagtcgaggg catggttgtt ggagagggac agcaggtcga acccggccct
     4381 ggagagggcg ggggccgcac ggtcgtcgcc ggtgaggttt agctggttag gggagggagg
     4441 gggcactcgg atcgagcctc ggcgcaccag cggactctca aggttgccca ccgcgaggtc
     4501 ggccggttgg agcagagcgc gcacgccctc aaacggggtg tcgtagcccc tggcagccag
     4561 ggtgctgccg acgtaccgcc ccagcatgag gtcgcccacg aacacaaggc tcacctcatc
     4621 gttggaaggg gccgaattgc cacctgccgt gcgcgcgccg ccaacctggg cgaaggcccc
     4681 ggccagcaag gcgacaacca gcgatatgga gatggcgatt ttgctcaagg tagttagctc
     4741 tgacgtggga agcggacacc tggcggacca ctcgtgtatg taaagtgaag ttgcgggtaa
     4801 aggtcgggag tatttccgcc gacgcagcta ttgtaccctg ttggcggatg tggataaagg
     4861 gcaggtcgga ttacggtgcg tacgaccaag ggagtacgtg gaggggatag aaagggacgc
     4921 cctacaatag ccctgtggct aaagccacgg gctgacaagt agcgaagtct ccttcggaga
     4981 ctagggctaa tctgcaaagt gctgcccgtg tgggaccagg ctacgccatt gtcattctga
     5041 gcgcagcgaa gaatctcgtc cttcagcacc caaacaccac ctttccccac ggcggtgcta
     5101 tgatgctgct atgttgctat cgaacagggg aacaccaggg tggaggacga gattcttcgc
     5161 tgcgctcaga atgacaatgg ccactcttgt tcgcttattt cttacagctt acagatactc
     5221 ccaagtcccc gtaggggact ttgctacttg tcagcccgta gatttagcca cagggctatt
     5281 gtagggcgag cgtggcggag caacctcgtc ctcccgctac agggcaggca gtgcaagttg
     5341 gaagctggtg ccctgaccgg gggaggaggt gacggtgagg gtgccgccgt gggcctcagc
     5401 gatgctgcgt gccaggtaga gaccgaggcc aagcccggtc gagtcggggc cggaggcgaa
     5461 gcgggtaaac aggcggggca tgagttcggg ggctatgccg gaaccctcgt ctctgatggt
     5521 gaggaccgcc cattcgccat cctcgcggcg ctcgctcgct agcccgacgt ctatggggac
     5581 gccctttgga gaatgtctga gggcgttcgc gatgaggttt tccaacgcct gcttgagacg
     5641 cgccgggtcg gcttctaccc gtaactcatc gggcagccgc aggaggatat ccagcctggc
     5701 ggtctgcatg accccggtgg cctcttctac caacgtgacg aggttcagcg gctcgcgcga
     5761 gaggctgaac actccctggt cgaggcgtgc cacgtccagc aagtcggtaa tgagggcttg
     5821 caggcggcgc acggcgcgaa gggcggcatc ggcgtcatcg atatcgcgcg gcctgtcctc
     5881 gcggtgggcg cggtccagga tgatggagag cctgcccttg aggggggtaa tgtaattgcg
     5941 caggtcgtgg gcgagcacgg tgacaagctc ctcggccacc acctgcctgg cctgcgccgc
     6001 cgcgtcctgg gcgatatgct ccaccagttc ggcccggtga gctaaagcgc ccacccaatg
     6061 acttacagcg tggaggaagc tgaggtcgtc ctcggagaaa gcgtcttgct tgctcgagca
     6121 ggcctggagc actccgcgcc gctcgccgtt gacgaccagg ggcacgatca tcatggaacg
     6181 tatacccagg cccttggtca tgccgggggg catagttgga tcactttccg cgtgcccgtt
     6241 gttgtaaaac tcgcccgtga agaaaacggc cgcttcgggc ccgtcgttag ccaggggcag
     6301 gagatgcata cctatctggt gctgccgcac gcccataggg gtatggctgg tgccttcggc
     6361 tacgagtgtc tcttttgtgg ggtccaggat gaaggcatcg gccttgtccg cgccgatagc
     6421 gcccacgatg agatcgctgg cctggtctag cgccgatttg aggttggtag cttctatcga
     6481 caggagttgt tccagcgtgg agagaagtct ttgctggccg tcgcgcttgt ccttcttgtc
     6541 catgtcgtgc cccattcatg gtaacacctc caacgggagc actggactgc taggcaaatg
     6601 ctggcgaaag aggttatgcg gttgtcctca tgatgacagc acataagtat acctgatttg
     6661 cgccagggtc cgtacggtgg gggaggcacg gcaggggacg ccctgccact a
//
LOCUS       DASRWZ010000019         7690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_618013, whole genome shotgun sequence.
ACCESSION   DASRWZ010000019 DASRWZ010000000
VERSION     DASRWZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618013"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..1805)
                     /locus_tag="VFH60_00240"
     CDS             complement(333..1805)
                     /locus_tag="VFH60_00240"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET6312234.1"
                     /translation="MNLDVTSQPLASLDTDLLVIGIAQDGPLSPLAQALDAEWGGLLG
                     TVRSEGAFKGKAYELEWLYPAPGKARRALLIGTGKASSYDARVVRRLAAIALRQARAK
                     KAGRVAIALPVAGSVTDALLVQSVADGALTGLADSDLYKDRENKGGVEGLQVAVAEAG
                     DEATHALERGRKIAVAVNFGRWLGDEPANVMTPVRAAEEARRWAAECGLECQVLGEDE
                     LREAGMGSLLSVSQGSKLPPQVVVLTYNGGGGPTLGLVGKGVTFDTGGISIKPAADMH
                     YMKYDMCGAAAVLSAIGGIAQLGLKANVIAVAGLVENMPSGTATRPGDVVTAANGKTI
                     EIINTDAEGRLVLADALYLARKRGAERLVDVATLTGAIKVALGDVATGAMGNNEEWTQ
                     RVLDAAKATGEALWPMPLYPEFTDKLKSNIADMMNTGGRFGGALTAGAFLQQFVGDTP
                     WVHLDIAGTAYTEKDYSWQMKGATGAMIRTLIALAEGELE"
     gene            complement(1823..2839)
                     /locus_tag="VFH60_00245"
     CDS             complement(1823..2839)
                     /locus_tag="VFH60_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003324180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6312235.1"
                     /translation="MTPNGTTADGGANDELYDLTIIGAGPTGLFAAFYAGMRTMKTKI
                     IDALPEPGGQLMALYPEKYIYDAPGFPKIVAKDLVRNLVEQATQWNPGMVLGERVLRL
                     EQATEGHWVLTTDKATHHSKAILIAAGVGAFAPNKLNAPGVTGLEGQAVYYFVKEKAA
                     FAGKDLLIVGGGDSAVDWALNLQGVAKSITVIHRRNEFRAHESTVTEMMNSSATVLTP
                     YEVREVSGDGKLEHVTIYNNKTDEEQTLHVDAILVNIGFKADIGPIREWGLRLDKRGI
                     VVNYRMETNLPGIYAAGDIAAEEVKMNLIATGYGQAAMAVNVAKNYIDPKASIFPGHS
                     SEKM"
     gene            complement(3063..5456)
                     /locus_tag="VFH60_00250"
     CDS             complement(3063..5456)
                     /locus_tag="VFH60_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6312236.1"
                     /translation="MDSRVMHPSQYASRRRTLFARLGAVLAWLVVVPFLFGSGSQAAT
                     RHPAPVGTAEPQATSVPATTLRDAFDAAAARWGVPVELLMAIGYVESHWEQRGGEASI
                     DGGYGIMHLVEGDAGTLQQAGHLTGHAEAELKADAATNIDGGAAALSYISQETKTTTK
                     QNNQWGRWYEVVATYSGAQDARVRREYAREVFRVLREGASVRLTSGDTLSLAATEGVQ
                     LPPSAADPQSPDSDDYADAIWVPAHANNYTSGRPYSQSSGLCSGIKYFVIHDTEGSYS
                     SAINWFQNPNSGVSAHYVIRSSDGQMTQMVRNADTGYHAGSWKYNICSIGIEHEGYMN
                     QDGWYTESMYQSSARLVATLADRYSMKKDRTRIIAHSEVPGATHQDPGPKWNWDYYMS
                     LVRHDADRSALVDNTDGGFSAVPSQIDADHAWWSYGGGYGGSSTLSTKSVTSHASSEN
                     SATWSAYLPSTGSYDVYAYVPYVDNNTADTENARYTLYTSEGTRVAAVSQKAITDSGE
                     GDWAHLGKFSFNAGTETRVYLDDYTGESGRNVWYDAIMWVPSGGGTPPPPAPTATETP
                     YRPPSPTNTPVPTKTATPLPQPTWTPGPGSMRFLDLPDTSWAYSYVANLFCRNVVSGF
                     PDGTFRPNSDITRGQFTKMLVLSFDLTWQVPDRATFSDVPLGSTYSPFVETAAKQGIV
                     AGYPDGTFRPNEPVMRGQAVKMIIVAHGGAQADPPVATFTDIPAQHWAFGYVEAAYRQ
                     GIINGYEDGTFRPQRGMTRAQVAKVLTLATQPWLPRSKPSGTSVPAQPPIPVVPLSK"
     gene            5867..6127
                     /locus_tag="VFH60_00255"
     CDS             5867..6127
                     /locus_tag="VFH60_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET6312237.1"
                     /translation="MTYVITEPCINVKDAACVDVCPVDCIHPRKDEPGFGEVLQLFIN
                     PDECIDCGACEPECPVNAIYPEDDVPEEYQEYTAKNREFFDL"
     gene            6237..>7690
                     /locus_tag="VFH60_00260"
     CDS             6237..>7690
                     /locus_tag="VFH60_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET6312238.1"
                     /translation="MAIIKSHVSTGTESYRENAGYMSALVSQLRERLEIVREGGGPDS
                     IARHRARGKLLARDRIEALLDPGTAFLELSPLAAWDMYDGDAPGAGVVTGIGVICGQE
                     CVIVANDATVKGGTYYPITVKKHLRAQEIALENHLPCIYLVDSGGAFLPLQAEVFPDR
                     DHFGRIFYNQANLSAKAIPQVAVVMGSCTAGGAYVPAMSDETVIVKGTGTIFLGGPPL
                     VKAATGEEVTAEELGGGEVHSRISGVTDHLAADDEHALAITRDIVANLHRRKELPWEV
                     EPPEPPLYDPAELYGIVEKDARRPYDVREVIARVVDGSRFHEFKALYGSTLVCGFARI
                     MGYPVGIIANNGILFSESALKGAHFIELCAQRRVPLVFLQNITGFMVGKEYENKGIAK
                     DGAKMVTAVATAQVPKFTVITGGSFGAGNYAMCGRAYQPRQLWMWPNARISVMGGQQA
                     ANVLLTVRRDSKELTPQEQAEFMKPTLDKYEEEG"
BASE COUNT         1353 a         2479 c         2344 g         1514 t
ORIGIN      
        1 accgcgcgag cagctttgtc gtccagccca cgccgcaggt agcggagcgt gccgggctag
       61 tcctgatacc agcagaatgg tcatgtcacc tggacgggtc cgaattggaa caaccaaaat
      121 agacgtaagc gagagcatca aagtaagggc aagaagaacc ggggcaacat gtgaaaagtg
      181 gcagcatcta ctttgagatg ttgcccctct gcactaataa accttcccga tctctgcgta
      241 gcctctgcgc cctctgcgtc tctgcggtga accgttcttg ctgcttctca gcgtggtcct
      301 tctaggaggt ctgctccacg agcggcgcgc gtctactcta gctcgccctc cgccagggca
      361 atcaaggtgc ggatcatagc gcctgtcgcg cctttcatct gccagctgta gtctttctca
      421 gtgtaggccg tgcccgctat gtcgaggtgc acccacggcg tgtcgcctac gaactgctgg
      481 aggaaggccc cagcagtcag cgcgccaccg aagcgtccgc cggtgttcat catgtcggca
      541 atgttgctct tgagcttgtc ggtgaactcc gggtagaggg gcatgggcca cagcgcttct
      601 cccgtggctt tggccgcgtc cagcacccgc tgcgtccact cctcgttgtt gcccatagcc
      661 ccggtcgcca cgtcaccgag ggccacctta atagctcccg tgagcgtcgc cacgtccacc
      721 aagcgctcgg ccccccgctt gcgggcaaga tacagcgcat cggccagtac gaggcggcct
      781 tcagcgtcgg tgttgatgat ctctatcgtc ttaccgttcg cggccgttac cacgtctccg
      841 gggcgggtgg ccgtgccgct gggcatattc tccaccagtc ccgcgacggc gatgacattt
      901 gccttcaggc cgagttgcgc gatgcctccg atagccgaga gcacggccgc cgccccgcac
      961 atgtcgtact tcatgtagtg catgtcagcc gctggcttga tggagatacc gccggtgtcg
     1021 aacgtgacgc ccttccctac gaggcccagt gtgggtccgc ctcccccgtt gtaggtcagc
     1081 actaccacct ggggcggaag cttgctgccc tgcgacacgc tcagcagcga acccataccc
     1141 gcctctcgca gctcgtcctc gcccagcacc tggcactcga ggccgcactc cgcggcccac
     1201 ctgcgggcct cttcggctgc gcgcaccggg gtcatcacgt tggcaggctc atcacccaac
     1261 cagcggccga agttcactgc taccgcgatt ttgcgcccac gctccagcgc atgtgtcgcc
     1321 tcgtcccccg cttccgcgac cgctacttgc aatccctcaa cgcccccctt gttctcccgg
     1381 tccttgtaga ggtcggagtc cgccaacccg gtcagcgcgc cgtccgcgac agactgcaca
     1441 agcagggcgt cggtcactga gcctgcgacc ggcagagcaa tagcaacgcg cccggctttc
     1501 ttagcgcgtg cctgccgcaa ggctatggcc gccagtcggc gcaccacgcg cgcgtcgtag
     1561 ctggaggctt tgcccgtacc tatcaacaga gcgcgacgcg ccttaccggg tgccgggtag
     1621 agccactcta gctcgtaagc cttgcctttg aacgcgcctt cgcttcgcac cgtgcctagc
     1681 aagccgcccc actcggcatc tagcgcctgc gctagggggg aaagcggccc gtcctgcgct
     1741 atgccgatta ccagcaagtc ggtgtctaag gaagcgaggg gttgcgatgt tacatctaag
     1801 ttcactgcgc ggaatctcct ggttacatct tctcgctgct gtggccgggg aagatgcttg
     1861 ccttcgggtc aatataattc tttgccacgt ttaccgccat tgcggcctgg ccgtagcccg
     1921 tggcgataag gttcattttt acctcttcag cggcgatgtc acccgccgcg tagatgccgg
     1981 ggaggttggt ctccatacgg taattgacca ctatgccgcg cttgtcgagt cgcagccccc
     2041 actctcgtat tgggccgata tcggccttga agccaatatt caccaggata gcatcgacgt
     2101 ggagcgtctg ctcctcgtcg gtcttgttgt tgtagatggt gacgtgctcc agcttgccgt
     2161 cgccggacac ctctcttacc tcgtaaggtg tcagcacggt cgcgctggag ttcatcatct
     2221 cggtgacggt gctctcgtgt gcgcggaact cgttgcgacg gtggattacg gtgatcgact
     2281 tagcgacgcc ctgcaggttg agcgcccagt ccaccgccga gtcgccgccg cccacgatga
     2341 gcaggtcctt acccgcgaag gcggccttct ctttgacgaa gtagtacacg gcctggccct
     2401 caagcccggt cacgcccggc gcgttgagct tgttaggcgc aaaagccccc accccggccg
     2461 caatcagtat cgccttggaa tggtgggtag ccttgtccgt cgtgagcacc cagtgcccct
     2521 cggtcgcctg ttccagcctc agcacccgct ctcccagcac catgccgggg ttccactgcg
     2581 tggcctgttc gacgaggttc cttaccaggt ccttcgccac gatcttcggg aagccgggcg
     2641 catcgtagat gtacttctcg gggtagagcg ccatcaactg accgccgggt tcggggagcg
     2701 cgtcgatgat cttggtcttc atcgtacgca tgcccgcgta gaaggccgcg aaaaggcccg
     2761 taggccccgc tccgatgatc gtcaaatcgt aaagctcgtc gttagcgcca ccgtccgccg
     2821 tggtgccgtt cggcgtcaca tgaggctgtc catccggtgc tgtggctccg ttgccagttc
     2881 gctccgtcga gtccatcaag tcctccattt cgcgtgttcc atgacagggt agcctttttg
     2941 aggccacgcc attatacctt acggggccag ccttaacaac cggaaacccg gccgaagtcc
     3001 aacaacaaga gccttcctgc acccgtgaca ggaaggctct tgttgctccg acagcgcatt
     3061 ggctacttac tgagaggtac cactggaatg ggcggctgcg ccgggactga cgtcccggaa
     3121 ggcttgctcc tggggagcca gggctgcgtg gcaagcgtga gcaccttggc gacctgcgct
     3181 cgcgtcatgc ccctctgagg ccggaatgtg ccgtcctcgt agccgttgat gatgccctgg
     3241 cggtaagctg cctccacata gccgaacgcc cagtgctgcg cgggtatgtc ggtgaacgtc
     3301 gcgaccggtg gatcagcttg agccccgccg tgtgccacga taatcatctt caccgcctgc
     3361 ccccgcatga ccggctcgtt cggtcggaac gttccgtcgg ggtagcccgc gactattcct
     3421 tgctttgcgg ccgtttccac gaacggcgag taggtgctgc ccagcggcac gtcgctgaag
     3481 gtcgcccggt cgggcacctg ccaggtcaga tcgaaactca gcaccagcat tttggtgaac
     3541 tgccccctcg taatgtcgga gttggggcgg aaggtgccat cggggaagcc cgaaaccacg
     3601 ttacggcaga agaggttagc cacgtaggag taggcccagc tggtatcagg caggtccaag
     3661 aagcgcatgc tgcccgggcc gggggtccac gtcggctgcg ggagaggcgt ggcggtcttt
     3721 gttggcacgg gcgtgtttgt tggcgagggt ggccggtacg gcgtttcggt cgcagtggga
     3781 gcgggtggag gaggtgtgcc cccgccggac ggcacccaca ttatggcatc gtaccagacg
     3841 ttacgcccgc tctcccccgt atagtcgtcg aggtacaccc tggtctccgt gcccgcgttg
     3901 aaggagaatt tgccgaggtg cgcccaatcg ccctcgcccg aatcggtgat cgccttctgg
     3961 ctgacggccg ccacccgcgt accttccgac gtgtaaagcg tgtagcgggc gttctcagtg
     4021 tcggcggtgt tgttgtccac gtaaggcacg taagcgtaga cgtcgtagct gcccgtactc
     4081 ggcaagtatg ccgaccacgt agccgagttc tcggagcttg cgtggctggt gaccgacttg
     4141 gttgataggg tgctagatcc gccgtaaccg ccgccgtagc tccaccaggc gtggtcggca
     4201 tcgatttgcg acggcaccgc ggagaagccg ccgtcggtgt tgtccaccaa cgcagaacgg
     4261 tccgcgtcgt gccggaccag gctcatgtag tagtcccagt tccacttcgg tccggggtcc
     4321 tggtgcgtgg cgcccggcac ctccgaatgc gcgatgatgc gcgtgcggtc tttcttcatg
     4381 ctgtagcggt cggcaagcgt agccaccaga cgggccgagg actggtacat ggactccgtg
     4441 taccagccgt cctggttcat gtagccctcg tgctcgatgc ctatagagca gatgttgtac
     4501 ttccaggagc cggcgtggta gcccgtgtcg gcgttgcgca ccatttgggt catctgcccg
     4561 tcagacgagc gaatgacgta gtgcgcactc acgccggagt tcgggttttg gaaccagttg
     4621 atggccgagc tatagctccc ctcggtgtcg tggatgacga agtacttgat cccgctgcac
     4681 agcccgctcg actgcgaata gggccggccc gaagtgtagt tgtttgcgtg ggcgggcacc
     4741 cagatggcat ccgcatagtc gtcgctgtcg ggcgactgag ggtccgcggc cgaaggtgga
     4801 agctggacgc cttcagtggc tgcgagggag agggtatcgc cggacgtaag tctgacgctc
     4861 gcgccctctc tcaacaccct gaacacctcg cgggcgtact cacggcgcac ccgtgcatcc
     4921 tgagcgccgg agtacgtcgc caccacctcg taccacctgc cccactggtt gttctgcttc
     4981 gtagtagttt tagtctcttg ggatatgtag ctcagcgccg ctgctccgcc gtcgatgttg
     5041 gtagccgcgt cggctttcag ttcggcctca gcatgtccgg tcagatgacc cgcttgctgg
     5101 agcgtgcccg cgtctccctc aacaaggtgc atgatgccat acccgccgtc gatggacgct
     5161 tcgcctccac gctgctccca gtgcgactct acgtagccaa tcgccatcaa tagctccacc
     5221 gggacgcccc agcgggctgc ggcggcatcg aatgcatctc gtagcgtggt cgccgggacc
     5281 gatgtggctt gcggctcagc tgtccccacg ggagcaggat gccgcgttgc cgcctggctg
     5341 ccggagccaa acaggaaggg caccacgaca agccacgcca ggacggcccc caaccgggca
     5401 aagagcgtgc gcctgcgact agcgtactgg gatgggtgca ttacgcgact gtccataagc
     5461 gacctcgtga gacttagact gtattgtcac tctgccatcc ccaccccctg accgagtttg
     5521 gggagatggc tcaatcaaat cgttgtacag atacgacgca tgatagaggc tatggttgcg
     5581 caaatccgct gacattatgt taagggcgtg gggcggccag cacaatacga aatttgtact
     5641 attgtttgcc ggtgacagga ttatgtcttg ttcttagggg atatgtactc taactttggc
     5701 gctggcccgg tgttcggcgt tgccaggcgc ccccaggcgt ggtcaacccg gctacgcacc
     5761 tccacacgtc ttgacacgcc gttgcacggg agtataatga tgcgtgaagc tacgcccttt
     5821 cacctggccc cggccggggc actactcaga ggaggacccg cgctaaatga cttacgtaat
     5881 taccgagcca tgcatcaacg tcaaggacgc cgcgtgcgtg gacgtgtgcc cggtggactg
     5941 cattcacccg cgcaaggacg aacccggctt cggggaagtg ctgcaacttt tcatcaaccc
     6001 cgacgagtgc atcgactgcg gcgcgtgcga gcctgagtgc ccggtgaacg ctatctaccc
     6061 cgaagacgac gttcccgagg agtaccagga gtacaccgcc aagaaccgcg agttcttcga
     6121 cctctgaccc ttcgggcgcg gcggaccacc cgcgcccgaa ttgccgatcg catggacctc
     6181 cggctggcct ccaccggggg tccagcaatg tcactcaagg aatttccaga ccccacatgg
     6241 caattatcaa gtcgcacgtt tcaacgggca cagagtcgta ccgggagaac gcaggctaca
     6301 tgtccgcact cgtttcccag ctgcgtgagc ggttggaaat cgtgcgggag ggcggcggcc
     6361 ccgactccat agcgcgccac cgagcgcgag gcaagctcct ggcccgcgac cggatagagg
     6421 cactcctcga cccaggcacg gccttcctgg agctatcacc cctagctgca tgggatatgt
     6481 atgatggaga tgcgccgggg gcgggtgtgg tgaccggtat tggggtgatc tgcgggcagg
     6541 agtgcgtaat cgtcgccaac gacgccaccg tcaagggcgg cacctattac cccataacgg
     6601 taaagaagca cctgcgcgcc caggagatcg ccctggaaaa ccacctgcct tgcatctacc
     6661 ttgtagactc cggcggtgcc ttcctgcccc tccaggcgga ggtctttccc gaccgggacc
     6721 actttggccg catcttctac aaccaggcca acctgtccgc taaagctatc ccccaggtcg
     6781 ccgttgtgat gggttcgtgc accgcgggcg gggcatacgt ccctgccatg tcagacgaga
     6841 cggtcattgt gaagggcacc ggcaccattt tcctgggcgg ccctcccctg gtaaaggcgg
     6901 ccacgggcga ggaggtaacc gccgaggaat tgggcggcgg ggaggtccac agccgcatca
     6961 gcggcgtgac cgaccacctg gcggcagacg acgagcatgc cctcgccatt acgcgcgaca
     7021 tcgtggcgaa cctccaccgt cgcaaggagc ttccgtggga ggtcgagcca cctgaaccac
     7081 cactctacga ccccgccgag ctttacggca tcgtagaaaa ggacgcgcgc aggccgtatg
     7141 acgtccgcga ggtcatagcc cgcgtcgtgg acggctcgcg cttccacgag ttcaaggcgc
     7201 tctacggcag cacccttgta tgcggcttcg cccgcatcat gggctacccg gtgggcatca
     7261 tcgccaacaa cggcatcttg ttctccgaaa gcgcgctgaa gggggcgcat ttcatcgagc
     7321 tttgcgccca gcgccgcgta ccgctcgtgt tcctgcaaaa catcaccggc ttcatggtcg
     7381 gcaaggagta cgagaacaag gggatcgcta aggacggcgc gaaaatggtc acggcggtag
     7441 ccacggccca ggtacccaag ttcacggtca tcaccggagg ctccttcggc gcggggaact
     7501 acgccatgtg cggccgcgca taccagccgc gacagttatg gatgtggcct aacgctcgca
     7561 tctcggtcat gggcgggcag caggcggcca atgtacttct taccgtgcgc cgggacagca
     7621 aggagctgac gccccaggag caagccgagt tcatgaagcc cacgctcgac aagtacgagg
     7681 aggaaggcaa
//
LOCUS       DASRWZ010000020         1794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_206899, whole genome shotgun sequence.
ACCESSION   DASRWZ010000020 DASRWZ010000000
VERSION     DASRWZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206899"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1794)
                     /locus_tag="VFH60_00265"
     CDS             complement(<1..>1794)
                     /locus_tag="VFH60_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312239.1"
                     /translation="FRLLRATSKLGVPGAMPGPPQLLPATTRVLAQGYLNRFSMPLLR
                     EWLLPPWMRRQSDPTSPLFTPRSVVNLMVNQTARNWTALGIPGRDHPVESLVDGWGLL
                     TPVPGGPSFDWWVDVEGGVGGTMSPATQRDVVQRLQGDLPVVLTAYEANGLRASSEVW
                     MLPLPEGDWAAMQVVLFNIADVRLRGTFSLALRPYNPEGISPIYRVSCDGATLVADGH
                     LGPVTWPAPDGWALSDISGDLFHRGAGAVSDRHSLHDVRGFAHGVLRYGFDIEPWEEA
                     EFLAFFPVHRGHTPDALRPAPKLFDVPQMEMRQPDSAYYSRAKASTTLAWREALDGGM
                     RVSLPHRELQASWEANRVHVLALHDGKTITPGPDLYHSFWLRDAAYMIYALSVCGYEE
                     AAAQLVRGLAAWQRRNGAFVSHNGEWDGSGQAMWAMERHLALHPDAGLRQELVPAVRR
                     ARRWIVRMLKVSEDGLMPPGISSEHLGPPDRYYWDRLWSLAGLRAADRLLGRRYVRGR
                     GAATSLQANIMKALRKDAQALGDYIVPAAPGRKIDLGMVGSLVGWFPLDCLPNSREEP
                     FARATLDALEAAHFHEGALFVNTGHSGWGTYL"
BASE COUNT          307 a          638 c          587 g          262 t
ORIGIN      
        1 aggtaggtgc cccagcccga atgccccgtg ttcacaaaga gcgcgccctc gtggaagtgg
       61 gccgcctcca gcgcgtcgag cgtagcgcgg gcgaacggct cctcccggct gttgggcagg
      121 cagtccagcg ggaaccaacc cacaagcgac cccaccatac ccaggtcgat tttgcggccc
      181 ggcgcggcag gcacgatgta gtcgcccagg gcctgcgcgt ccttgcgcag ggctttcatg
      241 atgttcgcct gtaacgaagt cgccgcgcct ctgcccctca catagcgcct gcccagcagc
      301 ctgtcggcgg cccgcaaccc cgcgagcgac cacaggcggt cccagtagta gcggtcgggg
      361 gggccgaggt gctccgacga aatgccgggc ggcatcaatc cgtcctcgga gactttgagc
      421 atgcgcacta tccagcgccg cgccctcctc accgcgggca ctagctcttg ccgcagcccc
      481 gcgtcagggt gcaaggccag gtgccgctcc atcgcccaca tagcctgccc tgagccgtcc
      541 cactcgccgt tgtgcgacac gaaagccccg ttgcgcctct gccaggccgc gagtccgcga
      601 acaagctgcg ccgccgcctc ctcgtaaccg cacaccgaaa gcgcatatat catgtaagcc
      661 gcgtccctga gccagaagct gtggtacagg tcgggaccgg gtgtgatagt cttgccgtcg
      721 tgcaacgcca gcacgtgcac gcggttggct tcccacgagg cctgtagctc gcggtggggc
      781 aggctgacgc gcatgccgcc gtccagcgcc tctcgccagg ctaacgtggt tgacgccttg
      841 gcccggctgt agtacgcgga gtccggctgc cgcatctcca tctggggtac gtcgaacagc
      901 tttggcgcgg gccgcagtgc gtcaggcgtg tgcccacggt gcaccgggaa gaaagccagg
      961 aactccgcct cctcccacgg ctctatgtcg aacccataac gcaacacccc atgcgcgaag
     1021 ccgcgaacgt catgcaagct gtggcggtcg cttacggcac cggcaccccg gtggaacagg
     1081 tcgccgctga tgtcggacaa cgcccagcca tcgggtgcgg gccacgtaac ggggccgagg
     1141 tggccgtccg ccaccagggt ggccccgtcg cagctgacgc ggtagatggg cgagatgcct
     1201 tcggggttgt aggggcgcag ggccaggctg aaggtgcccc tcaggcgcac gtccgcgatg
     1261 ttgaacagca ccacctgcat agcggcccaa tcgccctccg gcagcgggag catccacacc
     1321 tcgctcgagg cgcgcagacc gttggcttcg taggcggtaa gcaccaccgg caggtcgccc
     1381 tggaggcgct gcactacatc gcgctgcgtg gcgggagaca tggtgccccc caccccgcct
     1441 tccacgtcaa cccaccagtc gaacgacggc ccgccgggca cgggcgtgag caagccccac
     1501 ccatccacca ggctctcgac agggtggtcg cgccccggta tgcccagcgc ggtccagttg
     1561 cgggccgtct ggttcaccat gaggttgacc acgctccggg gcgtgaagag gggcgaagtg
     1621 gggtccgact gtcgccgcat ccacggtggc agcagccact cacgcaacag tggcatcgag
     1681 aagcgattga ggtagccctg cgcaagcacc ctcgtagtgg cgggcagcaa ctggggcggg
     1741 cccggcatcg cccccggcac gcccagcttc gaggtagcac gaagcaagcg aaag
//
LOCUS       DASRWZ010000021         3009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_1447,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000021 DASRWZ010000000
VERSION     DASRWZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1581
                     /locus_tag="VFH60_00270"
     CDS             268..1581
                     /locus_tag="VFH60_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET6312240.1"
                     /translation="MADTRTALVDKLRNREARVGIIGLGYVGLPLAVIFAEAGYHVTG
                     IDVDPRKVEALNSGESYIEDVPSEAIKPLVDGGLLDATTDFSVLREIDAVSICVPTPL
                     RKTRDPDVSFILAANEQVAKYLHPGMAIVLESTTYPGTTTELVLPRLREAGLTVGEDF
                     FLAFSPERVDPGRTDWTTRTTPKVIGGVTPACLEVALAFYGGAIEKLVPVSSTEAAEM
                     VKLLENTFRAVNIGLVNEVLLMCDKLGLDVWEVIDAAATKPFGFMKFTPGPGLGGHCI
                     PIDPLYLSWKLKTLNYNARFIELASEINTEMPIYWVQKVQDALNDEAKPVKGSKVLVL
                     GVAYKKDVSDIRESPAMDIILLLQEKGARVSYHDPHVPAFHHEALEMASVTDLEAALQ
                     DNDCTMIVTDHSSYDWDMVHRHARVLVDTRHALGGKPEPAGAEAR"
     gene            complement(1612..2205)
                     /locus_tag="VFH60_00275"
     CDS             complement(1612..2205)
                     /locus_tag="VFH60_00275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6312241.1"
                     /translation="MSDDVYIHPTADVSPRATLGAGTRIWHEAQVREGAVLGRNCILG
                     KGAYVDFGVRIGDNVKVQNRASIYHGVTLEDGVFVGPHVIFTNDKLPRAINPDGSLKS
                     DADWEVGQVLVKEGASLGAGAIIVAGVSIGRFALVGAGSVVTRDVPDFGLVYGNPARL
                     VGYVCENGHRAEETQEQRVGGRLWRCPSCGREVIVPP"
     gene            complement(2206..>3009)
                     /locus_tag="VFH60_00280"
     CDS             complement(2206..>3009)
                     /locus_tag="VFH60_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HET6312242.1"
                     /translation="ECAGNGRTGFSPLPQGEPGGVGAVATALWRGVPLAAILERAGLS
                     ASAVEVLFEGGDRGVPSAELGEVPFARSLPLEKALHPDTLLAYEMNGAPLPPAHGGPV
                     RLLVPGWYGMASVKWLSAVTALEQPFTGFYQRDRYVLDYPGSPETLPVREMAARALIT
                     DPEPGSVVRTGSRVVRGVAWSGRGNVASVEVSLEGGGPWRPARVLDPATPYTWRRWEF
                     EWQPTKAGRHVLRARAVDEQGNVQPDLAPWNRLGYCNNSVQGVVVEVRD"
BASE COUNT          549 a         1018 c          910 g          532 t
ORIGIN      
        1 gcccgtggct ttagccacag ggcgtcgtta tcgcgaccct gttccaggca ttactatgct
       61 ccaacaatac aaccattcga gcttcgctat cgtttcaact tcgatcctgc acaagcttcg
      121 cccctgttga cgtcctgtag ataaaatggc gtgcgggtgt tgaggtgatt acgcgccatg
      181 ttgtatcatt gataggttgt ggtgataaaa tggagataat tttctgtagg ctgttgctaa
      241 tagttaagga cacgcaagga acttcgtatg gctgacacaa ggaccgccct ggtagacaag
      301 ttgcgcaatc gcgaggctcg tgtgggcatt atcggtttgg gatatgtcgg gctgccgttg
      361 gcggttatct tcgcggaggc gggctaccac gtcacgggta tcgacgtgga cccgcgcaag
      421 gtggaagccc tcaactccgg cgagtcgtac atcgaggacg tgccgtctga ggctatcaag
      481 ccgctggtgg acgggggcct gctcgatgcc accaccgatt tctcggtgct gcgggagatc
      541 gacgccgtgt ctatctgcgt gcccactccc ctgcgcaaga cccgcgaccc ggacgtttcg
      601 ttcatcctcg cggccaacga acaggtagcc aagtacctgc acccaggcat ggcgatagtg
      661 ctggaatcta ccacctaccc cggcacgacc accgagcttg tgctgccccg gctgcgcgag
      721 gccggcctca ccgtaggcga ggacttcttc ctggccttct cgccggagcg cgtggacccc
      781 ggccgcaccg actggaccac ccgcaccact cccaaggtca taggcggcgt aacccccgct
      841 tgcttagagg tcgcgctggc cttctacggc ggggcgatag agaagttggt gccggtctcg
      901 tctaccgagg cggcggagat ggtgaagctg ctggaaaaca ctttcagggc ggtgaatatc
      961 ggcctggtga acgaagtgct cctgatgtgt gacaagctgg ggctggacgt gtgggaggtg
     1021 atagacgcgg cggccaccaa gccgttcggc ttcatgaagt tcaccccggg tccgggcctc
     1081 ggcgggcact gcatccccat cgaccccctg tacctctcct ggaagctcaa gacgctcaac
     1141 tacaacgccc gcttcatcga gcttgcttct gagataaaca ccgagatgcc catctactgg
     1201 gtgcagaagg tgcaggacgc cctcaacgac gaggccaagc cggtgaaagg ctcgaaggtg
     1261 ctggtgctgg gcgtggccta caagaaggat gtgtcggaca tccgggaatc gcccgcgatg
     1321 gacatcatcc tgctgctgca agagaaaggc gctcgcgtga gctaccacga cccccacgtg
     1381 cccgcattcc accacgaagc gttagagatg gcgagcgtca ccgacctaga ggccgccctc
     1441 caggacaacg actgcacgat gatcgtcacc gaccactcat cgtacgactg ggacatggtc
     1501 caccgccacg cacgggtgct ggtagacacg cgccacgccc tcggtggcaa gcccgagcct
     1561 gccggcgccg aagcacgcta gcaggtttct acttcagaag cggtctcgtc actacggagg
     1621 aactatgact tcccgcccgc aagaggggca ccgccagagc ctacccccca cgcgctgctc
     1681 ctgggtctcc tcggcccggt ggccgttttc gcagacgtag cccacgaggc gggccgggtt
     1741 gccatagacg aggccaaaat caggcacgtc cctcgtgact acagaccccg cacccaccag
     1801 ggcgaaccga cctatgctga cgcccgccac gataatcgct cccgccccca gtgacgcgcc
     1861 ttccttcacc agtacctgcc ccacctccca gtccgcgtcg ctctttagcg agccgtcggg
     1921 gttgatggcc ctggggagtt tgtcgttggt aaatatcacg tgggggccga cgaacacacc
     1981 atcctcaagc gtgaccccgt ggtagatgct ggcgcggttc tggaccttca cgttgtcccc
     2041 gatgcgcacg ccgaagtcca cgtacgcgcc cttgcccagt atgcagttgc gccccagcac
     2101 ggctccctcg cgcacctgtg cctcgtgcca tatgcgggtg cctgcaccga gggttgccct
     2161 gggcgaaacg tcggctgtcg ggtggatgta aacgtcgtca ctcatctagt ctcttacctc
     2221 aactactacg ccctgcaccg agttgttgca gtagccgagc cggttccagg gcgcaaggtc
     2281 gggctgcacg ttgccctgct catctacggc tcgagcgcgc aggacgtgcc tgcctgcctt
     2341 cgtgggctgc cactcgaact cccagcgccg ccaggtgtaa ggcgttgccg ggtcgagcac
     2401 gcgggcgggc cgccacgggc cgccgccctc caggctgact tccaccgagg cgacattgcc
     2461 tcgccccgac caggcaacgc cgcggactac acggctgccc gtgcgaacga ccgagcccgg
     2521 ctcagggtcg gtgattagcg ccctggcagc catttcgcgg acgggcagcg tctccgggga
     2581 accggggtag tcgagcacgt aacggtctcg ctggtagaaa ccggtaaagg gctgttcgag
     2641 cgcggtaacc gccgacagcc acttcacgga ggccatgccg taccagccgg gcaccaacag
     2701 acgcacaggc ccgccatgcg ccggcgggag gggcgcgccg ttcatctcgt aggccagcaa
     2761 cgtatcggga tgcagagcct tctctaacgg tagcgagcgc gcgaacggca cttcccccag
     2821 ctccgccgag ggcacgcccc tatccccgcc ctcgaacagc acctccaccg cgctcgcgct
     2881 cagccccgca cgctccagta tggccgccaa tggcacccca cgccagaggg cggtcgccac
     2941 ggcccccact ccccccggct cgccctgggg cagcggcgaa aagcccgtcc ggccgttgcc
     3001 cgcgcactc
//
LOCUS       DASRWZ010000022         5191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_275574, whole genome shotgun sequence.
ACCESSION   DASRWZ010000022 DASRWZ010000000
VERSION     DASRWZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275574"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5191
                     /locus_tag="VFH60_00285"
     CDS             <1..>5191
                     /locus_tag="VFH60_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HET6312243.1"
                     /translation="TFTASSLLSFSRSYVVVANGQVRPAKGAATELAGGSAANTWTFT
                     AADPTSVQGHSPDSSDGPSAPSNSFGFYFNNPLAPNQNVASFTSIDPTPEGYNNVLEI
                     DPGGTSVFTDQVKLLPNTTYRFQLRAGLKDKWGFDVAPASWTVNVGPLPPGLAIKGGG
                     FQPVYADGPSRVRIETVNLDKVTLHLHSLSEAELREIYTDPNVLYNPSPTYIGKLERE
                     WDVAVSKDNEGRATIFPAIALNAATDRLPVGFYFLRADAPTPYSRDLLQAYTVLMVGR
                     AGVVTKIERQDLLVWVADLGSGKPVPNYPLRVEQWRQDGQMPSQRRRTGQDGVLRLKL
                     DQFDDVTMRAVWSEQTGDAMFATTSWSSSIEPYAFGANVGYSTSMARAALYTDRPIYR
                     PAQVVYYRGAYRLDDDANYTLAPVGGTASLYASTYGNDGETVVYTGTATLSPAGTLNG
                     QFTLPANAPIGDYTLSLSRPGQTLDSNNRYDNVASASFQVQEYRKPDFQVNVAARDEA
                     VQGDPVTTTIQTSYYFGGPLANVTTTVNLQTSLYNFYWSDPDTGETYTFGEGYPRPYY
                     FDYGPYPGSTPEPVQSFQAQTSADGILDVDVSRYVSTTTGSKSLLIEGQVQDLSNQSV
                     ANSDTAIVHQGLYYVGLRVGDYVATAKKPLTITVRTVNSNAERIEPNRTVNLTFTRRD
                     WTAPRYGSGADWKLNEVQVGQVAVTTDANGRATYLFTPPSGGDYTLTAVSTDSRGNTI
                     RTTTQFWASSDGPGYVSWRYNNEKQIQLVADKTQYKIGDTARILVTSPFTQATGLLTV
                     ERGHIKRYRIINIEGGAPTIEVPLVDGDLPNVYVSIALLGPERAPEGAPADWANRATL
                     RMGYVNLSIDTSGKQVQVSIEPQGKTPFAPGSTAGIRVRTLDSSGRPVSGELSLAVVD
                     EAIYALAGDTSTDLFSAFWGERGTSVSTSSSFNAGDGWGMYYGGGRDYDSVPRTGGGG
                     MPLPEEAPMAGASVEAADMAASNQAAPSAPKKVRADFRDTAFWTASVTTGADGTATIN
                     VPLPDNLTTWRLTAQGITADTRAGMATQPMTVTQALLLRPVQPRFLTTGDSPHPQAII
                     HNNTAETLNLDVSLEVSGALTLETQPAATQQVSVAPGQQSIVTWSAKVGKGDVANLRY
                     WVRTADKSGSDYLEDAVSAQLPVKAFAAPEAVATSGEVSGTRADESVFLPYSVNPLLG
                     ELVVQVSPSLAAATTDSLQYVQEYPYESTDQTVSRFLPLVVLEQVYNEQGLTTPYSKD
                     IPAILERSFKRLANLQQLDGGWAWWERGPSVWWETAYAVQGLTAARDAGYAVPGNMLQ
                     RGISRLRDFMQENSSTQGIDETYRLNMRAYTLYVLSNAVGPDDEMRDEGRALAAQAPR
                     ISNHARAWLAMALAKNGLSSESKQVLDSLAAEAKQSSTTAHWEESSPDYWSMGTDNRA
                     TALAIDALVTLSPDDPLVPKAVRWLMTAEKEGHWLSSQETAIVLVSLAHYMRQSKELT
                     ADYTWQVDAFDKLLGQGAANTGNLTQTTTLRLPVADMPQNTLGNLSLARSGDKGKMYY
                     QISLRYYVPGEGIKSRSEGLAITRSYHKIAEASAGGQDSDPVTEVQASDLVKVRLMVV
                     APETSYYVLVTDPLPAGLEGVNGSLNTTSFTERPPNPMGTSLPDEGMGADSLYYDPWY
                     WRWGPFDNVEMRDDRTVLFATYMGPGTYVYEYYARATTPGVYMSLPAHAELLYYPDVF
                     GHSD"
BASE COUNT         1107 a         1701 c         1592 g          791 t
ORIGIN      
        1 gacctttacg gcttccagcc tgctcagttt cagccgcagt tacgtagtgg tggccaatgg
       61 gcaggtgagg cccgcgaaag gcgcggccac cgagctagct ggcgggagtg ccgcgaatac
      121 gtggaccttc acggcagccg accccacctc ggtgcaggga catagccccg actcaagcga
      181 tggcccatcc gcgccgtcta acagtttcgg cttctatttc aacaacccgc tcgccccgaa
      241 ccagaatgta gccagtttta cgagcataga tcccacaccc gaaggctaca acaacgtgct
      301 tgagattgat cccggcggta cgagcgtctt caccgaccag gtcaagctct tgcccaacac
      361 gacttatcgc ttccaattgc gcgcggggct gaaagacaag tggggcttcg acgtagcgcc
      421 cgcctcgtgg accgtcaacg tcggcccgct accccccgga ctcgcgatca agggcggtgg
      481 gttccagcct gtttatgcgg atgggccgag ccgcgtgcgt atcgaaacag tcaatttaga
      541 taaggtgacg ctgcacctgc attctctcag cgaagcggaa ctccgcgaga tatacaccga
      601 ccccaacgta ctctataacc cgtcgcccac ctacatcggc aagctagagc gcgaatggga
      661 cgtagcggtg agcaaagaca acgaaggccg cgccactatc ttcccggcca tcgccctgaa
      721 cgcggcaacg gaccgcctgc cggtgggctt ctatttcctc cgtgccgacg cacccacgcc
      781 ctacagccgg gatctgctgc aagcgtacac tgtgctgatg gtgggacgag caggggttgt
      841 caccaagatc gagagacaag acctgctggt gtgggtcgcg gacctgggca gtggcaaacc
      901 ggtgccgaac taccccctgc gtgtcgagca gtggcggcag gacgggcaga tgccaagcca
      961 gcgccggcgc acaggccagg acggcgtgtt gcggctgaag ctggaccaat ttgatgacgt
     1021 tacgatgcgt gcggtctgga gcgaacaaac gggcgacgcg atgttcgcca ccacctcttg
     1081 gagtagcagc atcgagccat acgcatttgg cgcgaacgtg ggctacagca cgagcatggc
     1141 gcgcgccgca ctctataccg acagaccaat ctaccggccc gcccaggtgg tctactaccg
     1201 gggcgcgtac cgcctggacg acgacgccaa ttacaccctt gccccggtgg gtggcaccgc
     1261 cagcctctac gcgagcacct acggcaacga cggggagacg gtcgtctata cgggcaccgc
     1321 gaccctatcc cccgcgggta cgctcaacgg ccaattcacg ctacccgcaa atgccccaat
     1381 aggcgattac accctttcgc tatcaaggcc gggccagacg ctcgacagca acaaccgcta
     1441 cgataacgtg gcgagcgcga gcttccaggt acaagaatac cgcaagcccg atttccaggt
     1501 gaacgtggca gcgcgtgacg aggcggtgca gggcgacccg gtcaccacca ccatccagac
     1561 gagttactac ttcggcgggc ctctggcgaa cgtcacgacc accgtcaacc tgcaaaccag
     1621 cctctacaac ttctattgga gcgaccccga cacgggcgag acctatacgt tcggtgaggg
     1681 ctacccgcgc ccatactact tcgattacgg cccttaccca gggagcacgc ctgagcctgt
     1741 gcagtccttc caggcgcaga ccagcgccga cggcatactc gacgttgatg taagccggta
     1801 cgtcagcacc accaccggca gtaaatcact gctgattgag gggcaggttc aggacctcag
     1861 caaccagtca gtagccaaca gcgacaccgc catcgtccac cagggcctct actacgtcgg
     1921 cctgcgcgtg ggggattacg tcgccacagc gaaaaagccc ctcaccatca cggtccgcac
     1981 cgtaaactcc aacgcagagc gcatagagcc taaccggacc gtcaacctga ctttcacacg
     2041 ccgcgattgg acagcgccac gctacgggag cggggctgat tggaagctga acgaggtgca
     2101 ggtcgggcag gtggcggtca cgacggacgc caatggccgc gccacctacc tcttcacccc
     2161 tccatcgggc ggcgactaca ccctgaccgc cgtcagcacc gacagccggg gcaacacgat
     2221 ccgcactacc acccagttct gggcgtcgag cgacgggccg ggctacgtct cctggcgcta
     2281 caacaacgag aagcagatac aactggtcgc tgacaagaca caatacaaaa tcggtgatac
     2341 cgcccgcatc cttgtgacat caccattcac ccaggccacc gggctgctga ccgtcgagcg
     2401 cggccacatc aagaggtacc gcatcatcaa catcgaaggt ggagcgccca ccatcgaagt
     2461 gccgttggtg gacggcgacc tgcccaacgt gtacgtcagc atcgccctgc ttgggccgga
     2521 gcgcgcacca gaaggcgcac ccgccgattg ggccaatcgc gccacattgc ggatgggtta
     2581 tgtcaacctg tccattgaca catcgggcaa gcaagtacag gtgagcatcg agccacaggg
     2641 caagaccccc ttcgcgccgg gcagcacggc aggcataagg gtacgcaccc tcgacagcag
     2701 tggccgaccc gtttcgggtg agctatccct ggccgtcgtg gacgaagcca tctacgcgct
     2761 ggcaggagat acgtcaaccg acctctttag cgcgttctgg ggcgagcgcg gcaccagcgt
     2821 cagcacgtcg tccagcttca acgcgggcga tgggtggggg atgtattacg gtggtggccg
     2881 cgactacgat tcggtgccca ggacaggcgg tggtgggatg cctctaccag aagaagcgcc
     2941 catggcaggg gcatcggtcg aggcagccga catggcggca agtaaccagg ctgcgccctc
     3001 ggcccccaag aaggtgcgcg ccgacttccg cgacaccgcc ttctggacgg cgagcgtcac
     3061 gacaggcgcg gatggcacag caacaataaa tgtccctctg cccgacaacc tcacgacgtg
     3121 gaggctcacc gcgcagggca tcacagccga cacgcgtgcg ggcatggcga cccaaccaat
     3181 gaccgtcacg caagccctgc tgctcaggcc ggtgcagccg cgcttcctca ccacgggcga
     3241 cagcccacac ccgcaggcga taattcacaa caacacggca gagaccttga accttgatgt
     3301 aagccttgag gtaagcgggg cgctcacact ggagacgcag cccgccgcca cccaacaggt
     3361 gagcgtagcg cccggccagc agtcgatagt tacgtggagc gccaaagtgg gcaagggcga
     3421 cgtggcgaac ctgcggtatt gggtgcgcac ggctgacaag agcggctcgg actatcttga
     3481 ggacgcggta tccgcccaat tgcccgtcaa agcctttgcc gcgccggaag cggtggctac
     3541 cagtggcgag gtgagcggca cccgcgccga cgagagcgtc ttcctgccat actcggtaaa
     3601 ccccttgctg ggcgagcttg tcgtgcaggt gtcgccatcg ctggcggctg ccaccaccga
     3661 cagcctgcaa tacgtgcagg agtacccata cgagagcacg gaccagacgg tgagccgttt
     3721 cctgccgttg gtggtgctcg aacaggtgta caacgagcag ggcctgacga cgccctactc
     3781 gaaggatata ccggccatac ttgagcgcag cttcaagcgg ctggcgaatt tgcagcagct
     3841 agacggcggc tgggcatggt gggagcgtgg cccgtccgtc tggtgggaga ctgcctacgc
     3901 ggtgcaggga ctcacggctg cacgcgacgc tgggtacgcg gtgccgggca acatgctaca
     3961 gcgcggtatc tcccggctgc gcgacttcat gcaagaaaac agcagcacgc agggcattga
     4021 cgagacctac cgcctcaata tgcgcgccta cacgctgtat gtgctctcca acgcggtagg
     4081 gccggacgac gagatgcgcg acgagggccg cgcgctcgcg gcgcaggcac cccgcatcag
     4141 caaccacgct cgcgcctggc tggcaatggc cctggcgaag aatgggcttt cgagcgagag
     4201 caagcaggta ctggacagcc tcgcggcaga ggccaagcag agcagcacca cagcccactg
     4261 ggaggagagc agccccgact actggagcat gggcacagac aaccgcgcaa ccgccctggc
     4321 gatagacgcg ctcgtcaccc tctcgcccga cgatcccctg gtgccgaagg cggtcaggtg
     4381 gttgatgacg gcggagaagg aaggtcactg gctcagcagc caggagacgg ctatcgtgct
     4441 ggtgagcctc gcccactaca tgcgccagag caaagagcta accgccgatt acacctggca
     4501 ggttgatgcc ttcgacaagc tcctgggcca gggtgcggcc aacaccggca acctgacgca
     4561 gaccacgacc ctgcggctgc ccgtagcgga catgcctcag aacacgctgg gcaacctctc
     4621 actggcgaga agcggcgaca aaggcaagat gtactaccag atcagcctgc gctactacgt
     4681 gcctggcgag ggcatcaagt cgaggagcga gggtctggct atcacccgca gctaccataa
     4741 gatagcagag gcgagcgccg gggggcagga tagcgacccg gtgacggagg tgcaggcgag
     4801 tgacctggtg aaggtgcgcc tgatggtagt cgcgcccgaa acgtcctact acgtgctcgt
     4861 gaccgacccg ttgcccgcgg gcctggaagg ggtcaacggc tcgctcaaca cgacatcgtt
     4921 caccgagcgc ccacccaacc cgatgggcac cagcctgccg gacgagggca tgggcgcgga
     4981 cagtttatac tacgacccct ggtactggcg gtggggaccg ttcgacaacg tggagatgcg
     5041 cgacgacagg acggtgctct tcgcgacgta catgggtccg ggcacatacg tctacgaata
     5101 ctacgctcgc gccaccaccc caggcgtcta catgagcctg cccgcccacg cggaactgct
     5161 ctactacccc gacgtgttcg ggcatagcga t
//
LOCUS       DASRWZ010000023         6719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_275601, whole genome shotgun sequence.
ACCESSION   DASRWZ010000023 DASRWZ010000000
VERSION     DASRWZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275601"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFH60_00290"
     CDS             complement(<1..392)
                     /locus_tag="VFH60_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312244.1"
                     /translation="MTRTITNFPARYPLTNEHVIEIEIVGWLTKEEAEVDPTYKPQWV
                     HEFSDFDFHVRLRVGRVYAVVASTPEFIREYMEREHEDSFLSPGLVIVRDISDTSFID
                     AVERWLYLGLNSHLGLEHYGCLQLADDEG"
     gene            complement(455..1492)
                     /locus_tag="VFH60_00295"
     CDS             complement(455..1492)
                     /locus_tag="VFH60_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6312245.1"
                     /translation="MRILIIGGTGFMGPLVVQRLFDMQHDITLFHTGRHEADLPPAVR
                     HIHSDLGGLPITGLPAELRALQPDVVLHMAPVGARDAQLVMDAFRGMAGRVVGISSMD
                     VYRAYGVLHDKEPGPLEPLPLTEDSPVRTHLYPYRASPHRPASDPEAWVDDYDKILVE
                     QAILGDSSLHGTILRLAMVHGPGDRQHRLFKYLKRMQDGRQAILLNEKGAKWRGARGY
                     VENIADAIALAATDERAAGRTYNVGEPDALSEAEWVAQIAAVVGWPGRIIEVPAHHLP
                     LAFNPDQHLVADTTRIRSELGYTESVPRPEALRRTIAWEYAHPPDTLNPSSYDYAAED
                     VILTEIERTTA"
     gene            2269..3582
                     /locus_tag="VFH60_00300"
     CDS             2269..3582
                     /locus_tag="VFH60_00300"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET6312246.1"
                     /translation="MARAEKDNIRFVNLQFTDLMGIVKSVAIPLHQFEDACLNGKWFD
                     GSSIEGFARIAESDMFLVPDLSTWAVMPWERTDESTARAICWVYNPNGDLFEGDPRHV
                     LARALDYAAALDYKLNTGPELEFFLFRMVNGKPSEDTHDMGGYFDLTGDMGADVRKDM
                     VNALEGMGIKVETSHHEVARGQHEIDFEYANALTTADSAVTLKYTLKAIAAQHGLHCT
                     FMPKPIFGINGSGMHTHQSLAGMDDPTNKFADADDPYGLSPIAKKYIAGQLKHARGMC
                     AVLAPLVNSYKRLVPGYEAPVYISWARTNRSALIRVPKNRPGKPQAARVELRCPDPSA
                     NPYLAFAVMLRAGLEGIEKNYEAPDPVEENLYHFDESERLRRNIQTLPGSLDEALREM
                     EQDELVRETLGDHITDRLLEAKRGEWDEFRMRVTQWELDRYLDVY"
     gene            complement(3718..4263)
                     /locus_tag="VFH60_00305"
     CDS             complement(3718..4263)
                     /locus_tag="VFH60_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312247.1"
                     /translation="MTTRNNPRSVTTRSISKRQLSATLVAAGAMLLLALLSIAALPAT
                     SQAAQPSSGPRPILQAEPTQALPTAQTDVTNQQGGSEEDNSLQGGSTENDSNSAPNSN
                     SNANEQNRPTDNLQGGSTEGGNPLGNTGGSDSPGGGFPWWIPIILVLLIAISIPFLRR
                     RPFNEPATNNPAPDLERRDQQ"
     gene            complement(4462..5457)
                     /locus_tag="VFH60_00310"
     CDS             complement(4462..5457)
                     /locus_tag="VFH60_00310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6312248.1"
                     /translation="MQRRRLGRSGIEVSPLGLGCWAIGGPVTLANGEERNPFGWGHVD
                     DEEAVRAIRRAIDLGVNFFDTANNYGAGHSERQLGRGIAGHRDEVVVCTKFATVFDEE
                     TSTVYFDREMDLTFDSIRETCEASLRRLNTDYIDLYLLHNGSLDPERVPPLLDHLERL
                     VDQGKIRWYGWSTDHADRAPAFAKGPHCTAIEFRLNLIYDNRQMLSVCDEHDLAAVIK
                     SPLNSGLLSGKFTADTTFPDDDGRKGLSFREGLGAERLRQIEAMRDTMTLDGRTMAQA
                     SLGYIWARSERTIPIPGFKTVAQVEENAAAIHCGPLTQQQVRRLDEIMGREPIEG"
     gene            5948..6490
                     /locus_tag="VFH60_00315"
     CDS             5948..6490
                     /locus_tag="VFH60_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET6312249.1"
                     /translation="MAIDATEQGAKRVLATGRVSAVCYSAELINGVGKEQHESAQVTK
                     WGIPGDRHYGETRVSRGRVVRNNRPITVVGADGTREACAKLGIPDIPPGGLGENILAE
                     GLGDLGGMAGGDEIHVLDARGEPKVILSVGKQNDPCASLMIYHKQIVKELMGRRGVLC
                     TVLLEGEVRVGDTIAWVRGE"
BASE COUNT         1217 a         1706 c         2448 g         1348 t
ORIGIN      
        1 ccttcgtcat cagctagctg taggcacccg tagtgttcca ggccaagatg gctattcagc
       61 cccagataca gccatcgttc taccgcatca ataaaagagg tgtcgctgat gtctcgaacg
      121 atcaccaggc ccggcgacag aaaggagtcc tcgtgctctc gctccatgta ctctctaatg
      181 aattccgggg tactggcaac gacggcgtac acacggccaa ctctaagccg tacgtggaaa
      241 tcaaaatcgc taaattcatg gacccattgt ggcttatagg ttgggtccac ctccgcctct
      301 tccttcgtca gccagccgac gatctcgatc tctataacat gttcgttggt gagggggtat
      361 cgagcgggga aattagtgat ggtgcgggtc atggtaatcg ctccactaat tcatcatcac
      421 gcactagacc actttgaatg tatctgaaag cgcctcaggc ggtagtacgc tcaatttcgg
      481 tgagtataac gtcttcggcg gcgtagtcgt agctggaagg gttgagggtg tcgggcgggt
      541 gagcgtactc ccatgctatg gtgcggcgga gggcttccgg ccggggtact gactcggtgt
      601 agcccaactc gcttcggatg cgggtggtgt cagcgacgag gtgttggtcg ggattgaagg
      661 cgaggggtag gtggtgcgcg ggcacctcga taatgcggcc aggccagcct acgacggcgg
      721 cgatttgggc gacccattcg gcttcggaga gggcatcggg ttcgcccacg ttgtaggtgc
      781 gcccggcggc acgctcgtcg gtggcggcaa gggctatggc gtcggcgatg ttttcgacat
      841 agccacgcgc accgcgccac tttgcgccct tctcgttcag caggatggcc tggcggccgt
      901 cctgcatgcg cttgaggtac ttgaagaggc ggtgctgacg gtcaccgggg ccgtggacca
      961 tggcgaggcg caggatggtg ccgtggaggg aggagtcgcc caggatggct tgttccacga
     1021 ggatcttgtc gtagtcgtca acccaggctt cggggtcgga tgcgggcctg tgaggggagg
     1081 ctcgataggg gtagaggtgg gtgcggacgg gggagtcttc tgtgaggggc agcggctcca
     1141 gaggccccgg ttctttgtcg tggagcacgc cgtaggcgcg gtaaacgtcc atgctgctga
     1201 tgccgacgac gcgaccggcc atgcccctga aggcgtccat gacgagctgg gcgtcgcgtg
     1261 cgcctacggg ggccatgtgg agcacgacgt cgggttggag ggcgcgaagc tcggcgggga
     1321 ggccggtgat tggtaggccg ccgaggtcgc tgtggatgtg cctgacggct gggggtaggt
     1381 ctgcttcgtg ccggcctgtg tggaacagcg ttatgtcatg ctgcatgtcg aagaggcgtt
     1441 gcacgactag cggccccatg aagccggtgc caccgatgat caggatgcgc atgtggtgcc
     1501 tccctgcctg gagcgggact ggtgcaaaca ggtgttagtc aatattttgg cgacgagagc
     1561 gcgtgacctt gtagaggata accattcttc gggatatcag gatgatttta tcacacctgg
     1621 cggtcagggc ttagagtgag agcggaacat tagcgtccat gtgccaccct tgtcatttcg
     1681 aacccttcgg ctgcgctcag gataaagtcc gtgagaaatc tcgtcctaca tcgggatgtg
     1741 gctttttctc accgggctga attgcgagac tgagagctgt aagaaaggtg gtatttgggt
     1801 gctgtgggac gagattcttc gcttcactca gaatgacata ggtgggggtt gggtgcgacc
     1861 ccaggagggg tcaaacgcgg ggggctgatc ccttcggctt tgctcagggc aggctcttcg
     1921 ctacgctcag aatgacgggg gagggttggg tactgatgtt cgatacggac attattggga
     1981 taagttttag cagttggccg tggattcgga cgtttgcaca aaaagtgggt gggagattct
     2041 gcgtttataa catagtgagg gtcaaagcgg gggtgctata ctggcttcgg tgggtagctg
     2101 tgaggcttct gccgatttta tttttgttgt agacctgaca ttcggaggtt tttcgagatg
     2161 gtgcaagaac tggaggctag agtgaacggc catcatacca aggctgaacc caaacgctac
     2221 tggacgcccg agacggcggg taataagcag tccgcggtgt ccgagatcat ggcgcgtgcc
     2281 gagaaagaca atatccgctt cgtgaatttg cagttcaccg acctcatggg catcgtcaag
     2341 tcggtggcta taccgctgca ccagttcgag gacgcgtgcc tgaacggcaa gtggttcgac
     2401 ggctcgtcta tcgagggttt cgcccgcatc gcggagtcgg acatgttcct ggtgccggac
     2461 ctctcgacgt gggccgtgat gccgtgggag cgcaccgacg agagcaccgc ccgcgccatc
     2521 tgctgggtgt acaacccgaa cggcgacctg ttcgagggcg acccgcgcca cgtgctggcc
     2581 cgcgccctgg actacgcggc ggcgctggac tacaagctga acacggggcc ggagcttgag
     2641 ttcttcctgt tccgcatggt gaacggcaag ccgagcgaag acacgcacga catgggcggc
     2701 tacttcgacc tgacgggcga catgggcgcg gacgtgcgca aggacatggt gaacgccctg
     2761 gaagggatgg gcatcaaggt cgagacctcg caccacgagg tggcgcgggg gcagcacgag
     2821 atcgacttcg agtacgccaa cgccctcacc acggcggaca gcgcggtgac gttgaagtac
     2881 acgctcaagg cgattgccgc gcagcacggg ctgcactgca cgttcatgcc caagccgatc
     2941 ttcggcatca acggctcggg catgcacacg caccagagcc tggcggggat ggacgacccg
     3001 accaacaagt tcgcggacgc ggacgacccg tacgggttgt cgcccatcgc gaagaagtac
     3061 atcgcggggc agttgaagca cgcacgcggc atgtgcgccg tgctcgcgcc gttggtgaac
     3121 tcgtacaaga ggctggtgcc cggctacgag gccccggtgt acatctcgtg ggcgcgcacc
     3181 aacaggtcgg cgctcatcag ggtgcccaag aaccgccccg gcaagccgca ggccgcccgc
     3241 gtggagctgc gctgccccga cccctcggcc aacccgtacc tggcgttcgc ggtgatgctg
     3301 cgggccgggc tggaaggcat cgagaagaac tacgaagcgc cggacccggt ggaggagaac
     3361 ctgtaccact tcgacgagag cgagcggctg cgccgcaaca tccagacgct gcccggctcg
     3421 ctggacgagg cgttgcgaga gatggagcag gacgagctgg tgcgcgagac gctgggcgac
     3481 cacatcaccg accggctgtt agaggccaag cgcggggagt gggacgagtt ccgcatgcgg
     3541 gtaacgcagt gggagttgga ccggtatttg gacgtgtatt gattttggat tttaggtttt
     3601 ggattttgga tttggttggt tgttgaggta ggtccgggat gccgggcggt aattgaagat
     3661 ggtgagaagg ggccgcttcc tggggagtgg ccccttgtta tttatgaaag ggccggttta
     3721 ctgttggtcg cggcgttcga ggtcgggggc gggattgttg gtggcaggct cgttgaaggg
     3781 gcggcgtcgc aggaagggta tggagatggc aatgagcagg acaaggatga tgggtatcca
     3841 ccaggggaag ccgccgccgg ggctgtcgga gccgccggtg ttgccgaggg ggttgccgcc
     3901 ttcggtgctg ccgccttgca ggttgtcggt ggggcggttt tgctcgttcg cgttggagtt
     3961 gctgttgggg gccgaattgc tgtcgttctc ggtgctgcca ccctgcaagg aattgtcctc
     4021 ttcgctgccg ccctgctggt tggtcacgtc ggtctgggcg gtggggaggg cttgcgtggg
     4081 ctcggcttgt aggatggggc gggggccgga ggaaggttgg gctgcctggc tagtggcggg
     4141 tagggcggcg atggagagca gagctagcag gagcatggcc ccggctgcta cgagagtcgc
     4201 tgatagctgg cgcttgctga tgctcctggt tgttacagag cgtgggttgt tgcgagttgt
     4261 catgccgggg cctccgaggt gctggaatgc atagtacggt gtgtcgtgcc cccccgcgca
     4321 tggaacaagc aatggttatg ccagctagag gaagtaatga gtgatgagga ctgaggactg
     4381 agtagagcgg gacgcaaaga aggtgatggt taggccgcgt gctgctcagt cctcagtcct
     4441 cgtcactcat tacttccctg tctatccttc gattggttct cggcccatga tttcatcgag
     4501 gcggcggact tgctgttggg tgagggggcc gcagtggatg gcggcggcgt tttcctcgac
     4561 ctgggcaaca gtcttgaagc cggggatggg gatggtacgc tcgctgcgcg cccagatgta
     4621 gccgagggag gcctgggcca tggtgcggcc gtcgagggtc atggtgtcgc gcatggcttc
     4681 gatctggcgg aggcgttctg cccccagccc ctcgcggaag ctgaggcctt tgcgcccgtc
     4741 atcgtcgggg aaggtggtat cggcggtgaa cttgccgctg aggaggccag agttgagggg
     4801 gcttttgatc acggcggcga ggtcgtgctc gtcgcagaca gaaagcatct ggcggttgtc
     4861 atagatgagg ttcagccgga actctatggc ggtgcaatgc ggcccctttg cgaatgcggg
     4921 ggcgcggtcg gcgtggtcgg tgctccagcc gtaccagcgg attttgccct ggtccaccag
     4981 gcgctcaaga tggtcgagca gagggggcac gcgttcggga tcgaggctgc cattgtgcag
     5041 caggtagagg tctatgtagt cggtgttgag gcggcgcagg ctggcctcac aggtctcgcg
     5101 gatggagtcg aaggtgaggt ccatttcgcg gtcgaagtag accgtcgatg tctcctcgtc
     5161 gaacacggtg gcgaacttgg tgcagacgac cacctcgtcg cgatgcccgg cgatgccccg
     5221 gcccagttgt cgctcgctgt gtcccgcgcc gtagttgttg gctgtgtcga agaagttgac
     5281 gcccaggtcg atagcgcggc ggatggcgcg taccgcctcc tcgtcgtcta catggcccca
     5341 gccgaagggg ttgcgttcct cgccgttcgc aagcgtcacg gggccgccta tggcccaaca
     5401 gcccaggccg agggggctga cttctatacc gctacgtcca agtcttcgtc gttgcatttt
     5461 cgtgtaaggc tcctttcggg tatgtgtgag gcgcgcagac cgatggcgct gcggggttcc
     5521 agccgtgggc cgcgggaagc aggacaaggc ggggcaggct gctccaatgc cgcgtggagc
     5581 aggtaagccg gacggccggg tcgccgtgga gctgccgcgc tatgggaggt cgaaggaggg
     5641 aagcgttggc ggcggtacgg tggtaggcgt gggaacagtt acaggcgggg ccaggcagag
     5701 gtagtggcgg ggtgcgggtc cgctactgag ggacaaggcg taggggtggg gcgagtgctc
     5761 tgccggtctg tgttgtgcca tcacgtatat agtagtgcgc gttgggggta aaaggttcac
     5821 tctgtggggt tggaaagggt gctgggggcg gtgtatacta ttggcagcgg caagggaggc
     5881 gatgggactt tatgggggcg tgtagtggaa gatggctcct ctaccatgcc cgtgatggag
     5941 cgcattcatg gcgatagatg ctacggaaca gggtgctaag agggtgctgg ctacgggcag
     6001 ggtgtcggcg gtctgctaca gcgcggagct aataaacggg gtgggcaagg agcagcacga
     6061 gtcggcgcag gtgacgaagt ggggcatacc gggcgaccgg cattacggag agacgcgggt
     6121 gagccggggg cgcgtggtgc ggaacaaccg gcctataacg gtggtggggg ccgacggcac
     6181 gcgggaagcc tgcgcgaagc tggggattcc cgacatccca ccgggcgggc tgggcgagaa
     6241 catcctggcc gaagggctgg gcgacctggg cggtatggcc gggggcgatg agatacacgt
     6301 gctggacgcg cgcggcgagc cgaaggtgat actgagcgtg gggaagcaga acgacccgtg
     6361 cgctagcctg atgatctacc acaagcagat agtgaaggag ctaatgggca ggcgcggggt
     6421 gctgtgcacg gtgctcctcg aaggcgaagt gcgcgtgggc gacacaatag cgtgggttag
     6481 gggtgaatag ttaataggac gatgaaaccg caaaggcgca aagagcgcaa aggacaccaa
     6541 tgattaagta ggtgttctac gcaaaaggac cagggtacat cagtcgtacc ctggtccttt
     6601 ttgtacccac taaacaatcc ttaaactttg cgctctttgc gcctttgcgg tttctcctcg
     6661 tcacaacttg cccaatgcac tgtagccgga tagaataggg tatagctggc gaaggggga
//
LOCUS       DASRWZ010000024         4656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1029416, whole genome shotgun sequence.
ACCESSION   DASRWZ010000024 DASRWZ010000000
VERSION     DASRWZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029416"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..605)
                     /locus_tag="VFH60_00320"
     CDS             complement(33..605)
                     /locus_tag="VFH60_00320"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET6312250.1"
                     /translation="MAREDLKILTNRPAINHEQAAEVLFNCASLLQMAGANEYRIAHY
                     RDAARTMLRLGPVALEAALNEELWPQLGLGKRLGNKVRELVRDGAMQFYVELRADLPP
                     AVDQLMRVNGVGPKLATRLHKELGVNTPAELADAARHGQVAKLRGFGPKRQAAYARIL
                     GPNPPTSPAVGTIRLLPREDEEQYELPAAA"
     gene            1162..2607
                     /locus_tag="VFH60_00325"
     CDS             1162..2607
                     /locus_tag="VFH60_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6312251.1"
                     /translation="MSKIELEGATSVEAPPDALAGLSAPDLTDGNRQRSFRRHSFPNG
                     LKLITKEVHAAPVVSFWVWYRVGARDEHIGITGISHWVEHMMFKGTSTLGKGRIMQMV
                     AENGGTLNAFTSDDWTTYFETLPSDRLDLGIMIESDRMANSLFDPAEVESERTVIISE
                     REGHENEPDYMLNEEVSATAFKVHAYGNGIIGWKSDLQTMTREDLYNHYKTYYAPNNA
                     TVVVVGDFDTDELIAKLDEAFGGYEPSQNIPQVRGMEPEQKGERRVIVRHPGATAQLE
                     IVYHTPSASDPDIYPLMVADALLSGAKPLGFGGAGMGRSARLYKSLVATEIAVSAGSY
                     FALHKDPNLFVFGASARQTDDPEGKLREIEDALLEQVRKLQEGDITTEELEKAVRQSR
                     AQFIYSGDSVSSQAYMFGFLESIHTADMYDEALDRLAAVTPQDVQRVARQYFTADNRT
                     VGWFIPLDEESGEDSGTTDASQDGAPSGVES"
     gene            2609..3901
                     /locus_tag="VFH60_00330"
     CDS             2609..3901
                     /locus_tag="VFH60_00330"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6312252.1"
                     /translation="MQTVAERATRGLGPETITRRVLDNGMTVLVYPNPTVPSLVARVS
                     LKGGAMFDPPEKAGLASFAVRSLRRGTERRTFEQLNEDTEGRGASVGVDAGQTLIEAG
                     GRSLKEDTGFLLETIAEVLLQPSFPEAEIEKLRLQLRTGLVEMESDTGSVAELAFRQA
                     LYPDGHPFHYRVAGFLSTLDNIHRDDMLAFYRRYFRPDRALMVVVGDVEPEDIVSRAQ
                     DLFGGWRAEGEVEPYVVPDVAQPVGETRVHRFVPGKTQNDIVLGFPSMRRSDPDYYAF
                     DLMNLLLGRIGLMGRLGKTVRDEQGLAYYAGSSFEAGIGRGPWAVRAGVNPSNVNKAI
                     DSIKSEIERMRTEPIPDEELEGGKRYMTGVLPLRLETSDGISRSILEMELYGLGFDYI
                     TSYPGIINALTPEYVNEVAAMRMSSENYVAAIAGPNVS"
     gene            complement(4209..>4656)
                     /locus_tag="VFH60_00335"
     CDS             complement(4209..>4656)
                     /locus_tag="VFH60_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET6312253.1"
                     /translation="VVAAAEAGAAGVVEASASAGAAEGSPVVASVAVPAAEASVEVAR
                     PAAVGAAVGRQAGVGKAMKVRKQIYITAEQDAELKRLSYATGESESEIIRRALTSFLR
                     RTTAMPGRTRAWQEETKFMQSLLSEDYGPEPDGRTWTREELYSREN"
BASE COUNT          924 a         1441 c         1454 g          837 t
ORIGIN      
        1 acaataagca gctaattcgc cgtaacgccg gattacgcag ccgcaggcaa ctcgtactgc
       61 tcttcgtcct cacgcggcag caggcgaatg gtgccgactg cgggactggt aggcgggttg
      121 gggccgagga tgcgggcgta agcggcctgg cgcttgggac cgaagcccct cagcttggct
      181 acttggccgt ggcgggcggc gtccgctagc tcggcggggg tgttcacgcc cagctctttg
      241 tgcagcctcg tggcgagctt ggggcctacg ccgttcacgc gcataagctg gtccacggcg
      301 ggcggcaggt cggcgcgcag ctccacgtag aactgcatgg caccgtcgcg cacaagctcg
      361 cgcaccttgt tgcccagcct cttgccgagg ccaagctgcg gccatagctc ttcgttcagc
      421 gccgcttcca acgccaccgg gccgaggcgc agcatagtgc gggcggcgtc gcggtagtgg
      481 gcgatgcggt actcgttggc cccggccatc tgcagcaggg aagcgcagtt gaacagcact
      541 tcagcagcct gctcgtggtt gatagcgggc ctgttcgtca gtatcttcag gtcttctcta
      601 gccatctttt tgctcacttc ttgtaacaca ataactcggc tctgtttacc acatatctgg
      661 aactcttgtg ctaattatac acgtaaacga cagaaaagtc aagagcatga gggcaaaaag
      721 gtcgaacttg caactagctt ttttcagcta caaatactat ctagaattcg aatacaaata
      781 ttatctagaa ttcgaacaaa tatcgctcaa acggcccgca tcgtgtcgcc aggatggtca
      841 gtggcgctgc cttatccgcg gtcggatagg agaacccttg gtcccccttt gtcattctga
      901 gcgtagcgaa gaatctagag aagccggtac agggaggcta ttgaacacca cgtaccacgg
      961 tgctcatgct caacgttgaa gccgtgccag catcttggat tcttcactac gttcagaatg
     1021 acacggtcct ccttggtcct gctgttcggc ctgttagcgt cacgcgttgc ggggttcagt
     1081 gcctcggctg tgcgcgtcgg ttataatgga ggcaagtatc acgagcggtc aaaatgggac
     1141 aaaacaggag aaaggcatag catgagcaag atcgaattag agggtgcgac ttccgtagaa
     1201 gcgcccccgg atgccctcgc aggactgtca gcgccggacc ttacggacgg gaatcgtcag
     1261 cgcagcttcc ggcggcattc gttccctaac gggctgaagc tgataaccaa agaggtacac
     1321 gccgcccccg tggtatcgtt ctgggtctgg tacagggtgg gggcgcggga cgagcatatt
     1381 ggcatcacgg gcatctcgca ctgggtcgag cacatgatgt tcaagggcac cagcacgctc
     1441 ggcaaaggcc gcatcatgca gatggtggcc gagaacggcg gcacgctcaa cgcctttacc
     1501 tccgacgact ggactactta cttcgagacc ctgccctccg accgcctcga cctcggcatc
     1561 atgatcgagt ccgaccgcat ggcgaactcc ctgttcgacc cggcggaggt cgagtcggag
     1621 cgcacggtga tcatctcgga gcgcgagggc cacgagaacg agccggacta catgctcaac
     1681 gaggaggtgt cggccaccgc gttcaaggtg cacgcctacg gcaacggcat catcggctgg
     1741 aagtccgacc tgcaaacaat gacccgcgaa gacctctaca accactacaa gacttactac
     1801 gcgcccaaca acgccacggt ggtcgtggtg ggcgacttcg acacggacga gctgatcgcc
     1861 aagctggatg aggcgttcgg cggctacgag ccaagccaga acatcccaca ggtacgcggg
     1921 atggagccgg agcagaaggg cgagcggcgc gtaatcgtgc gccaccctgg ggccaccgcc
     1981 caactggaaa tcgtgtacca taccccctcg gcctccgacc ccgatatcta cccgctgatg
     2041 gtggccgacg cgctgctgtc gggtgccaag ccgctcggct tcggcggtgc gggcatgggg
     2101 cgctcggcgc ggctgtacaa gtcgctggtg gcgaccgaga tagctgtgag cgcaggttcc
     2161 tacttcgcgt tgcacaagga ccccaacctg ttcgtgttcg gggcaagcgc ccggcagacg
     2221 gacgaccccg aaggcaagct gcgcgagata gaggatgcct tgctggagca ggtgcgcaag
     2281 ctacaggagg gtgacatcac caccgaggag ttggagaagg cggtccgcca gtcccgcgcc
     2341 cagttcatct attcgggcga cagcgtatcg agccaggcgt acatgttcgg cttcctcgag
     2401 agcatccaca cggcggacat gtacgacgag gcgctggaca ggctcgccgc cgtgacaccg
     2461 caggacgtgc agcgagtggc ccgccagtat ttcaccgccg acaaccgcac cgtgggctgg
     2521 ttcatcccgc tcgacgaaga gagcggcgag gatagcggca caacggacgc atcgcaggac
     2581 ggcgcgccca gcggagtgga gagctaagat gcagaccgta gcagagaggg ccacgagggg
     2641 actcggcccc gaaacgataa cgcgccgcgt gttggacaac ggcatgacgg tgctggttta
     2701 ccccaacccc accgtcccgt cgctcgtcgc ccgcgtgtcg ctcaagggtg gggccatgtt
     2761 cgacccgccg gagaaggcgg ggctggcctc gttcgcggtg aggtcgctgc gccggggcac
     2821 cgagcgtcgc acgttcgagc agttgaacga ggacaccgag ggccggggcg cgtcggtggg
     2881 cgtggacgcg ggccagacgc ttatagaggc tggcggcagg tcgctcaagg aggacaccgg
     2941 cttcctgctg gaaacgatag ccgaggtgtt gttgcagcct tccttcccgg aagccgaaat
     3001 cgagaagctg cgcttgcagt tgcgcacggg cctggtcgag atggagagcg acaccggctc
     3061 tgttgccgag cttgcgttca ggcaggcgct ctacccggac ggccacccgt tccactaccg
     3121 ggtagcgggc ttcctcagca cgctcgataa catccaccgc gacgacatgc tggccttcta
     3181 ccggcgctac ttccgccccg accgcgccct catggtggtg gtgggggacg tcgagccgga
     3241 ggacatcgtg agccgggcgc aagacctgtt cggcggctgg cgggccgagg gcgaagtgga
     3301 gccgtacgtc gtacccgacg tggcgcagcc ggtgggcgag acgcgcgtgc acaggttcgt
     3361 gccgggcaag acccagaacg acatcgtgct cggcttcccc tccatgcgcc gctccgatcc
     3421 cgactactac gccttcgacc tgatgaacct gctgctgggc cgcatcggcc tgatgggcag
     3481 gctcggcaag acggtgcgcg acgaacaggg ccttgcttac tacgccggtt cgtcgttcga
     3541 ggcggggatt ggccgcgggc cgtgggcggt gcgcgcgggt gtgaacccat ccaacgtcaa
     3601 caaggcgata gacagcatca agagtgagat cgagcgcatg cgcaccgagc cgatacccga
     3661 cgaagagctt gaaggcggca agcgctacat gacgggcgtc ctccccctgc gccttgagac
     3721 gagcgacggc atctcgcgct cgatactaga gatggagctt tacggcctcg gcttcgacta
     3781 catcaccagc taccccggca tcatcaacgc cctgacgccg gagtatgtga acgaagtcgc
     3841 ggctatgcgg atgtcgtcgg agaactatgt cgcggcgatt gcggggccga acgttagctg
     3901 ataagcaaga gccaggttat taagccacaa gagagccgtg tttccccaag gtgtagacgc
     3961 ggctctgctt ataggctggc cctaaagtgt gcctgcaaac tgtagggaac aggcgaacaa
     4021 ggtagcccct gtcattctga gcgtagcgaa gaatctaaga tgctgcccct acctcgacgt
     4081 taaacgtcag cacctcagta cgtgacattc aacagcatcc ctgtgcagac ttctttagat
     4141 tcttagctac gctcagaatg acaggggcta ggcttggtct cccacgggcg gcactttgca
     4201 ggtggaccct agttctcgcg actgtacaat tcttcgcgag tccaggtgcg accgtctggt
     4261 tcaggcccgt agtcctcgga taatagcgat tgcatgaatt tggtttcctc ttgccacgcc
     4321 cgcgtgcggc caggcatggc agtcgtccgg cgcaaaaagc tggtcagcgc ccgccgtatt
     4381 atctcagatt ccgattcgcc ggtagcgtaa gacaatcgct tcaactcggc gtcctgttcg
     4441 gctgtgatgt agatttgctt gcgcactttc atggccttac caacccccgc ttgacgaccc
     4501 accgccgccc caaccgccgc cggacgagcc acctccaccg aagcctccgc tgctggaacc
     4561 gccaccgaag ccaccaccgg agaaccctcc gccgcccccg ccgatgctga agcctccacc
     4621 accccggctg ccccagcctc cgccgctgct acgacg
//
LOCUS       DASRWZ010000025         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_824013, whole genome shotgun sequence.
ACCESSION   DASRWZ010000025 DASRWZ010000000
VERSION     DASRWZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824013"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2056
                     /locus_tag="VFH60_00340"
     CDS             <1..>2056
                     /locus_tag="VFH60_00340"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HET6312254.1"
                     /translation="GLIVGSLYTTNTWDFPTYLVIVLAGLGLPYLAAQRQTEQSRGWA
                     WLRPWIVQSALIVLLAIITFLPFHLTFKSLVGGQPVQLPENLANIPVVSWILEKLATL
                     LLVNTADKTILGFLVIFGIFLAALLVWLAYEAVGYARRQKESGANGNATLYIFLGVIA
                     ATLVLALLFRFPLLALLLPIGFVSLYIVWREPERTERNLALVMVGVAALIGLVIEVVF
                     LRDNFQMRMNTIFKFYYQIWVLWAVAAGYALWRTLHAALREREGQDERGRITLTTAPT
                     GIRAMSGVWAIFFLLLAFSGLLYGYYGPMSRQVAGGGQRLRGLDGTTWLRDASPGDHA
                     GIVWLKANASGNDTVLEAGRDEYGRPGRVSSYTGVPTLVAWDNSHEALWRTGQPELRD
                     QIGTRRQVVNSIYQGVDPANGAPLTAQRLLELLKQYNVTYVFVGATERGEPGAAPASA
                     NEQVTQYAEQVFKQAMPEAFRSGSTVIYAVKDGIAGTGEAPPTPVAGTTPPPAGQFDP
                     NAPPAGLFDRVPAGPNRGQFNFPRGIAADAEGNFYVVDTHNLRIQKFDRDGKFVTMFG
                     SKGNGNGQFLPFSDEAEGTGPGGIAVDAAGNLYVADTWNHRVQKFDRDGKFLAQWGSF
                     INMADASATADPDANRKFFGPRGVAIGPDGDVYVTDTGNKRVLIFGPDGSYKRQISSG
                     MSP"
BASE COUNT          388 a          624 c          663 g          381 t
ORIGIN      
        1 ttggcctcat cgtcggctct ctctatacga ccaacacctg ggacttccct acatacctgg
       61 tgatagtgct ggcgggcctg ggcttgccct accttgctgc tcagaggcaa acagagcaat
      121 caagaggctg ggcctggttg cgcccctgga tcgtacaatc tgccctgatc gtcctcctgg
      181 cgataatcac gtttctgccc ttccatctca ccttcaagtc gctggtgggc gggcaacccg
      241 tgcaattgcc cgagaatctt gcgaacattc cagttgtaag ctggatattg gagaagctgg
      301 cgacccttct gcttgtcaac accgccgaca agaccatcct gggcttcctg gtgatattcg
      361 gcatcttcct ggcggccctc ctggtgtggc tggcctatga ggcggtgggc tatgcgcgcc
      421 gccaaaagga gtcgggggcg aacgggaacg cgacactgta catcttcctg ggcgtgatag
      481 ccgccacatt ggtgctggct ttacttttca ggttcccact gctcgcatta ttgctcccca
      541 tagggtttgt atcgctctac atcgtctgga gggaaccgga gcgcacggaa cggaacctcg
      601 cgctggtcat ggtaggcgtg gccgcgctca ttggcttggt gatagaggta gtcttcctga
      661 gagacaactt tcagatgagg atgaatacca tcttcaagtt ctattaccag atatgggtgc
      721 tgtgggcggt cgcagccggg tacgcgttat ggcggacgct gcacgccgcc ctgcgcgagc
      781 gtgaagggca ggacgagcgg ggccgcatca cactcactac ggctccaaca ggcatccggg
      841 ccatgtccgg tgtgtgggcc atcttcttcc tcttgctggc gttctccggc ctgctctacg
      901 gctactacgg gccgatgagc aggcaggtgg cgggtggcgg gcagcggttg cgcgggctgg
      961 acggcacgac gtggctgcga gatgcatcgc ccggcgacca tgcgggcatc gtgtggctca
     1021 aggccaacgc gagcggcaac gacacggtgc ttgaggcggg gcgcgacgaa tatggcaggc
     1081 cagggcgcgt gtcgtcctat acgggcgtgc ccacgctggt tgcctgggac aactcccacg
     1141 aggccctgtg gcgcaccggc cagccggagc ttcgggacca aatcggtacg cgcaggcagg
     1201 tagtcaactc catctaccag ggggttgacc cggcgaacgg cgcgcctctg accgcacaga
     1261 ggctgcttga gttgctcaag cagtacaacg tcacatatgt attcgtgggc gcgacggagc
     1321 gaggcgagcc aggggcggca cccgccagcg ccaatgagca ggtcacgcag tacgccgaac
     1381 aggtcttcaa gcaggccatg ccggaggcgt tcaggtcagg ctcaacggtc atatacgcgg
     1441 tcaaagatgg gatagcgggc acgggtgaag cgccgcccac gcccgtagcc ggaacgacgc
     1501 ccccgcccgc cgggcagttc gaccccaacg cgccgcctgc cggtctattc gaccgcgtac
     1561 ccgccgggcc gaatcggggc cagttcaact tcccgcgtgg gatagcggcg gacgcggaag
     1621 gcaacttcta cgtggttgac acccacaacc tgcgaataca gaagtttgac cgggacggca
     1681 agttcgtcac catgttcggc tcgaagggca acggcaatgg acaattcctg cccttctcgg
     1741 acgaggccga aggcacgggt ccaggtggca tagcggttga cgcggcgggc aatctctacg
     1801 tggccgacac ctggaaccac cgggtacaga agttcgacag ggacggcaaa ttcctcgcgc
     1861 aatgggggtc gttcatcaac atggccgacg cttccgcgac cgccgacccc gacgccaatc
     1921 gcaagttctt cgggcctcgc ggggtggcga tagggccgga cggcgacgtg tacgttaccg
     1981 atacgggcaa caagcgcgtg ctgatattcg gccccgacgg cagctacaaa cggcagatta
     2041 gcagcggcat gagtcc
//
LOCUS       DASRWZ010000026         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_412831, whole genome shotgun sequence.
ACCESSION   DASRWZ010000026 DASRWZ010000000
VERSION     DASRWZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412831"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFH60_00345"
     CDS             <1..253
                     /locus_tag="VFH60_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6312255.1"
                     /translation="ILAVTGILTFIGVYGEFLIARLMLRDRQEFTLMVGLFLFASDRS
                     QEWGIITAAALVGALPVVIFFFLTQRQIVSGLSSGAVKG"
     gene            384..638
                     /locus_tag="VFH60_00350"
     CDS             384..638
                     /locus_tag="VFH60_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312256.1"
                     /translation="MNDTRRSFLTRLGRVKDAVAGWLPFRSGGDVAAAPLAGKLKDLS
                     WSDPEDEETARRQAAGQAGGDPLARTIYNARPQNPFDKDF"
     gene            639..2444
                     /locus_tag="VFH60_00355"
     CDS             639..2444
                     /locus_tag="VFH60_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HET6312257.1"
                     /translation="MPDETLSLASAKRLRGGQRPHVPPPSSPTSTWVRIEFEGGPDAY
                     PEAVFTDPRREHGWRVALSWDGGANLWQADVLLPQEPTVLTYHFVMRDGSTLRERHQV
                     EGEVEMLYGVWEEREFQVAVYDPNDTPPAWLPGTIFYQIFPDRFAQSDPANVRKGGTT
                     YGQEPLYLDWDDKPEHPPKGRDFFGGDLRGVIDKLDYIAGLGVTCIYFTPIFASPSNH
                     RYDATDYSLVDPRLGTEDDLRELIREAKARGVRVLLDGVFNHCSSDSIYFQEAVRSKE
                     SPYYRWFDFVHWPERWVGWLGMKHKLTTLGVKTMPEFVECPEVEEFFFGEGGIALRWL
                     SLGTAGWRTDVTPWMTHEFWRRFRRAVRRSYPDVYLVAEDWASVPQRLVGDSFDATMN
                     YGLGFSILGFANRKLSAAELDDRLETRRRDTPPASFHAQMNILGSHDTPRVLHKVEGS
                     KERLVFANALLLAYPGAPMIYYGDEAGVEGSYAEDGRRPYPWGKEDPQLLAFYRTAIN
                     ARRDSAALGKGDVATAWIDDRGGYGFIRTHGEERVLALFNNGDTALEAAIPVGDNVPD
                     GESVDLLSLLPAARIAGGTLYASIPALGAAWYRLP"
     gene            2507..2863
                     /locus_tag="VFH60_00360"
     CDS             2507..2863
                     /locus_tag="VFH60_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312258.1"
                     /translation="MDSDDRIERRDDDRQIERLDAADEGTAQGPAAASVISANLAGIN
                     TGISTAATGAPSAGNIGGFSGAAAEAVLRGDAEDEEAVAMNLDALEQFGSKHRQTGDD
                     GVSTEQSELEADELNG"
BASE COUNT          559 a          929 c          938 g          456 t
ORIGIN      
        1 catcctggcc gtgacgggca tcctcacttt catcggcgtg tatggcgagt tcctcatcgc
       61 ccgccttatg ctgagggaca ggcaggagtt tacgctgatg gtgggcctct tcctgttcgc
      121 cagcgaccgc tcgcaggaat ggggcatcat caccgccgcc gcgctcgtgg gcgctctgcc
      181 tgtggtgatc ttcttcttcc tcacgcagcg gcagatagtg agcggtctct ccagcggcgc
      241 ggtaaaaggc tagaggttgg aagacctcaa ttacaagtga atgcacgggg gcgtgaggta
      301 tcacgccccc gtgctgcgcc tccgtaatgc tagcaacaat acaagggcga cctacgtaca
      361 tgttgcatgt aaggaggcac ccaatgaatg acacaagacg cagtttcctc acaaggctcg
      421 gccgtgtgaa ggacgcggtt gccggatggc tgccatttcg ctctggggga gacgtagcgg
      481 ctgcaccttt agcaggcaag ctgaaggacc tgagctggag cgacccggag gacgaagaga
      541 cggcccgcag gcaagcggca ggacaggccg ggggcgaccc cttggctcgg acaatctaca
      601 acgcacgccc acagaacccg tttgacaagg acttctaaat gcccgatgag accttgagcc
      661 tcgcctccgc caaacgcctg cgtgggggcc aacgtcccca tgtgccgccg ccctcctcgc
      721 ctacgtccac ctgggtgcga attgagtttg aaggggggcc ggatgcttac cccgaagctg
      781 tgttcaccga cccgcgccgc gagcatggct ggcgggtggc cctctcgtgg gatggcgggg
      841 ctaacttatg gcaggcggac gtgctcctcc cacaggagcc gaccgtgctc acctatcact
      901 tcgtcatgcg cgacggctcc acgttgcgcg agcggcacca ggtagagggc gaggtggaga
      961 tgctctacgg cgtgtgggag gagcgcgagt tccaggtcgc cgtgtacgac cccaacgaca
     1021 ccccgcccgc ctggctcccc ggcaccatct tctaccagat attccccgac cgcttcgccc
     1081 agagcgaccc cgctaacgtc cgcaagggcg gcaccacgta cggccaggaa cccctatacc
     1141 tcgactggga cgacaagccg gagcacccac ccaagggccg ggacttcttc gggggcgacc
     1201 tgcgcggggt catcgacaag ctcgattaca tcgccgggtt gggcgtgacc tgcatctact
     1261 tcacccctat ctttgcttcc cccagcaacc accgctacga cgctaccgat tacagcctgg
     1321 tagacccgcg cctgggcacg gaagacgacc tgcgcgagct tatacgagag gcgaaggcac
     1381 gaggcgtacg ggtgctgctc gacggcgtgt tcaaccactg ttcgagcgat agcatctact
     1441 tccaggaggc ggtacgcagc aaggaatcgc cctactaccg ctggttcgac ttcgtgcatt
     1501 ggccggagag gtgggtgggc tggctcggca tgaagcacaa gcttaccacg ctgggcgtaa
     1561 agacgatgcc ggagttcgtg gagtgccccg aagtcgagga gttcttcttc ggtgaggggg
     1621 gcatcgcact gcgctggctc tcgctcggca ccgcgggctg gcgcaccgac gtgaccccct
     1681 ggatgacgca cgagttctgg cggcgcttca ggcgtgccgt gcgccgctcc taccccgacg
     1741 tctaccttgt ggccgaggac tgggcgagcg tgcctcagcg cctggtgggg gacagcttcg
     1801 acgccaccat gaactacggc ctcggcttca gcatcctggg cttcgcaaac cgcaagctga
     1861 gcgccgccga gcttgacgac cgcctcgaaa cgcgccgccg cgacacccca cccgccagct
     1921 tccacgccca gatgaacatc ctcggctccc acgacacccc gcgcgtcctc cacaaggtgg
     1981 agggcagcaa agagcgcctg gtgttcgcaa acgctctgct gctcgcgtat cccggcgcgc
     2041 cgatgatcta ttacggcgac gaggcagggg tggaaggcag ctacgcggaa gacggccgcc
     2101 gcccctaccc gtggggcaag gaagacccgc aactgctcgc cttctaccgc acggccatca
     2161 acgcccgccg cgatagcgcc gccctgggaa agggagacgt tgctaccgcc tggatagacg
     2221 accggggagg ctacggcttc attcgcactc acggcgagga gcgcgtgctc gccctcttca
     2281 acaacggcga caccgcactg gaagccgcca tacctgtagg tgacaacgta cccgatgggg
     2341 agagcgtaga cctgctgtcg ctccttcccg cggctcgcat cgcaggaggc acgttgtacg
     2401 ccagcatacc ggcgttgggt gcggcctggt accggctccc ataatccggg ggcctgagca
     2461 gacggacatc gcatgtagca caagtagcga ttggagggca aacgagatgg acagcgacga
     2521 caggatcgaa cgacgagacg acgacaggca aatcgaacgg ctcgatgcgg ctgacgaggg
     2581 cacggcgcaa ggaccggcgg cagccagcgt gatctcggct aacctcgcgg gcatcaacac
     2641 gggcataagc accgcggcta cgggcgcacc gtccgcgggc aacatcgggg gcttttcagg
     2701 ggcggcagcc gaggcggtcc tgcgtgggga cgcggaggac gaggaagcgg tcgcgatgaa
     2761 cctggacgcg ctggagcaat tcggcagcaa gcaccggcaa acgggcgacg acggcgtctc
     2821 cacggagcag agcgagctag aagcggacga gctgaacggc tagaggcagc gcaggggagg
     2881 tt
//
LOCUS       DASRWZ010000027         4746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_618441, whole genome shotgun sequence.
ACCESSION   DASRWZ010000027 DASRWZ010000000
VERSION     DASRWZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618441"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="VFH60_00365"
     CDS             complement(<1..1706)
                     /locus_tag="VFH60_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET6312259.1"
                     /translation="MKSRSRLALSGVLCLLAFFASGPGNEWRAQAQVLVNPLPEGREG
                     LPPGVSIETVLTGMDRPVAMAFDPQGRLFYTEKDTGNVRLFANGTLQPNPVINFKVDR
                     NNERGLLGIALDPDFSRNHFVYVYYTCLVGPDCASSENRVVRFVESNGTGSNPATIFT
                     SPATAPESQHNGGNIHFGPDGKLYISVGDDGNAANSQDVTVKHGKMHRINPDGSAPGD
                     NPSFKQPGALPSLYAMGLRNSFDFTFDPVVPGRIFATENGRECDDELNRIEAGFNYGW
                     RTGYLCDDQDPGGLDPQYNTIAPLWTSGRGDGGRKVPTGITVYTGDQIAQWRNDLFMA
                     TYQQPRRLYHFSLNADRTALTAVNVVPEVLAHMDLETGPDGALWYIQGGGFEEGTLKR
                     LVNMGAAPTSQASPTATVVPGTPAPVAIPGNGNRTFPETGKTVRGIFLDYWDTHGGLP
                     QQGFPISELMAEVSDLDGKTYTVQYFERAVFEYHPQQQPPFNVLLSQLGTFQYRKKYP
                     NGAPNQQANTSPGSVLFPETGKRVGGKFLEYWQRNGGLAQQGYPISEEFTEKSELDGK
                     EYR"
     gene            2253..3005
                     /locus_tag="VFH60_00370"
     CDS             2253..3005
                     /locus_tag="VFH60_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET6312260.1"
                     /translation="MSRVAPLYELQQVDTALQSRVSRMRQIDESMADSPELLAARSAA
                     EEAARYLAGLQDRLRKSSEAVDETSRRIKTQDKRLYDGSIKNPKELGQVQEEVGHLKT
                     RLKQQEDELIDLMLSVERAEEASREKQAALEAVEHDTEQYRAGLTEEKDKLLQQAKVL
                     QVKRQRLVNEAAWADLQAYERLRRAKGGHAVSEVRDGKCGGCGVRVPVNVVRAARADS
                     DFAYCPTCGRMLYPIGDLRFQEFDHNLDNVDR"
     gene            3092..4540
                     /locus_tag="VFH60_00375"
     CDS             3092..4540
                     /locus_tag="VFH60_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HET6312261.1"
                     /translation="MRNPALVAAVAAGKASALATRLMGKGGGTALPGLVAHTIYPSVL
                     RALTSQLPEGSIMVTGTNGKTTTARMLGSILEAAGLDPLHNRSGSNLERGLVSTMVDA
                     ATPGGGLPGSLRAGLFEVDEAALPGVLPAIRPKVALFNNLFRDQLDRYGEIDTVYRKW
                     RDALPAMRPRSTVVLNADDPAIAALTLAPNLRANVFTFGIDDPRYSLDALPHAADSIS
                     CPRCNSRLDYSLILLGHLGHWRCPSCGLARPRLDVVAKQVRLHGTESSDLELETPVGS
                     MQVTLHVPGLYNVYNGLAAVSAALAFGVAPHYIKEGLDRFTAAFGRIERVQIPGSNDN
                     ALLMALVKNPVGFNEVLRMLFPLEEREGLAPAAPKHLMIVINDLFADGRDVSWLWDVD
                     FEILSATLDLVGRVQVSGIRAGDMAVRLKYAGVDPTLIKLDVDIAGALENAVRALPAG
                     ETLYVLPTYTAMLAFRKMLYEKGWVHSQFWEQ"
BASE COUNT          899 a         1458 c         1495 g          894 t
ORIGIN      
        1 cggtactcct taccgtctag ctcgctcttc tccgtgaatt cctcagatat ggggtagccc
       61 tgctgcgcca gccctccatt gcgctgccag tactccagga acttgccccc taccctctta
      121 cccgtctccg ggaagagcac cgagcctgga gaggtgttcg cctgctggtt gggtgctccg
      181 ttggggtact tcttccggta ctgaaacgtg ccaagttgcg acaggagcac gttgaaaggc
      241 ggctgctgct gggggtggta ctcgaacacg gcacgctcga agtactgcac cgtgtaggtc
      301 ttaccgtcca ggtccgatac ctcggccata agctcagata tggggaagcc ctgctgaggc
      361 agccctccgt gtgtgtccca gtagtcgagg aagatgccgc gcaccgtctt acccgtctcc
      421 gggaaggtgc ggttgccgtt gccgggtata gcgactgggg caggtgttcc aggtaccacc
      481 gtagcagtag ggctggcttg tgaagttggt gcggcaccca tgttcaccag gcgcttgaga
      541 gtaccttcct cgaagccgcc accctggatg taccacaagg ccccatcagg ccccgtttcg
      601 aggtccatgt gcgccaggac ttccggcacg acgttcacgg cagtgagtgc cgtccggtcg
      661 gcgttcagag agaaatggta cagcctgcga ggctgctggt aggttgccat gaagaggtcg
      721 ttgcgccact gcgctatttg atcgcccgtg tataccgtaa tgcccgtggg cactttgcgg
      781 ccaccatccc cgcggcccga ggtccacagc ggcgcaatcg tgttgtactg agggtctaag
      841 ccgccggggt cctggtcgtc gcacaagtag cccgtccgcc agccgtagtt gaagcccgcc
      901 tcgattcggt tcagttcgtc gtcacactcg cggccgttct cggtggcgaa tattctcccc
      961 ggaaccaccg gatcgaacgt gaagtcaaag ctattgcgca ggcccatagc atacagggaa
     1021 gggagcgccc ctggctgctt gaagctcggg ttgtcgccgg gcgcgctgcc atccgggttg
     1081 atccggtgca tcttcccgtg cttcacggtc acgtcctgcg agttggcggc gttgccatcg
     1141 tcgcctacag atatgtatag cttgccgtcc gggccgaagt ggatgttgcc gccgttgtgc
     1201 tggctttcag gagcggtcgc gggcgaggtg aatatagtag ccgggttgga gcctgtgccg
     1261 ttgctctcaa cgaagcgcac cacccggttc tctgagctgg cgcagtccgg cccaacgagg
     1321 caggtgtaat agacgtaaac gaagtggttg cggctgaagt ccgggtctaa agcaatgccc
     1381 agcagaccgc gctcgttgtt cctatcgact ttgaagttga taaccgggtt cggctgcaac
     1441 gtcccattgg cgaagaggcg cacgttgccg gtgtcctttt cggtgtagaa gagcctgccc
     1501 tggggatcga acgccattgc cacgggcctg tccatacccg tcagcactgt ctcaatcgac
     1561 acgcccggcg gcaacccctc cctgccctca ggaagcggat tcaccagaac ctgggcttgc
     1621 gcccgccact cattgccggg accggacgca aaaaaggcca gcaggcagag cacgcctgac
     1681 agggccagcc tggaacgaga tttcatagtg aagctcctta cgacaaatac gcgataacct
     1741 gtctgcctgt agcgcgcggc gaagcgggct acgcaagaca aatcgcgggt cttacgtcgc
     1801 atagtggagc gaaactaccg gcaggtacgt gacagtagcg tgcaaaccgt gctctcaagg
     1861 ccggcggcag acccaggcaa gcacctggga aggtacctag cgcgccttac ggtgcagcat
     1921 gtcgttcagc atcaatatcg gaacgcgggc gcgcaaagta accggccggg ggcactgcat
     1981 ggtctctgcg atagccctgt ggggacgccc tgtggctgaa gccacgggct tagaagaggc
     2041 aaagtcccat gggggactga ggaacatcat gcagtctccg taggagactt cgtccctggt
     2101 aagcccgcca attcattggc agggcatcac cgcagggcga cccctaccgg acagcccgca
     2161 ctccgtctct ccatccgcac ctcgcttgcc cgctctatag gggtgtgtta tcctatctaa
     2221 gagtatggcc ttccccctac taaggagccg ttttgagtcg cgtagcacct ttatacgaat
     2281 tgcagcaggt agacaccgcg ctacagagcc gcgtgtcccg catgcggcag atagacgaga
     2341 gcatggccga cagccccgaa ctgctggccg ccaggagcgc cgccgaggag gccgcccggt
     2401 accttgccgg cctgcaagac cgcctgcgca agtcttccga ggccgtggac gagacttcca
     2461 ggcgcatcaa gacccaggac aagcgcctgt acgacggctc tatcaagaac cccaaggagc
     2521 tggggcaggt ccaggaggaa gtaggccacc tcaagacccg gctcaagcag caggaagatg
     2581 agttaatcga cctcatgctc tcggtggagc gagcagagga ggccagccgg gagaagcaag
     2641 ccgcccttga agctgtggag cacgacaccg agcagtacag ggccgggctg accgaggaga
     2701 aagacaagct gctacagcag gccaaggtgc tacaggtcaa gcgccaacgc ctcgtcaacg
     2761 aagcggcgtg ggccgacctg caggcttacg agcggttgcg ccgcgccaag gggggccacg
     2821 ccgtgtccga agtgcgcgac ggcaagtgcg gcggctgcgg ggtgcgggtg cctgtcaacg
     2881 tggtcagggc agcgcgcgcc gacagcgatt ttgcctactg ccccacctgc ggccgcatgc
     2941 tctaccccat aggcgacctc cgcttccagg agttcgacca caacctcgac aacgttgata
     3001 ggtgatttgg aaattcgaat ttcggatttc gagtttagta tgtatccaac aagttcgaaa
     3061 tccgaatttc gaatttccaa atggaggaaa catgcgtaat ccggcgctgg tggcggccgt
     3121 ggcggcgggg aaggcttcgg cgctggctac ccgcctgatg ggtaagggtg gcggcactgc
     3181 cttgccgggg ctggtggcgc acactatcta tcccagcgtg ctgcgcgccc ttacctccca
     3241 actgcccgaa ggctccatca tggtcacggg caccaacggc aagactacca ccgcgcgcat
     3301 gctcggctcg atacttgagg ccgccgggct ggaccccctg cacaaccgct ccggctccaa
     3361 cctggaacgc ggcctggtgt ccactatggt agacgcggct acccccggcg gtggcttgcc
     3421 gggcagcctg cgggcgggcc tgttcgaggt agacgaggcg gccttgcccg gtgtgcttcc
     3481 cgccatccgc cccaaagtgg ccctgttcaa caatctcttc cgcgaccagc tagaccgcta
     3541 cggggagata gacaccgtgt accgcaagtg gcgcgacgcc ctgcccgcca tgcgtccccg
     3601 ctccactgtg gtgctcaacg ccgacgaccc ggctatcgcc gctttgacgc tggctcccaa
     3661 cctgcgcgct aacgtcttca ccttcggcat tgacgacccg cgctactccc tcgacgcgct
     3721 gccccacgcc gccgactcca tttcgtgccc gcgctgcaac agccgcctcg actacagcct
     3781 gatcctgctg gggcacctgg ggcactggcg ctgtccgagc tgcggccttg cacgcccacg
     3841 gctggatgta gtggcaaagc aggtgcggct gcacggcacc gagagcagcg acctggagct
     3901 tgagacgccg gttgggtcta tgcaggtgac cttgcacgtg ccggggctgt acaacgtgta
     3961 caacggcctg gcggcggtat ccgcggcact ggcgttcggg gtggccccgc actatataaa
     4021 ggaagggcta gaccgcttca cggctgcgtt cgggcgcatc gagcgggtgc aaataccggg
     4081 cagcaacgac aatgccctgc tgatggcgct cgtcaaaaac ccggtcggct tcaacgaagt
     4141 gctgcgcatg ctcttccccc ttgaagagcg cgagggtctg gcccccgccg cgcccaagca
     4201 cctgatgatc gttatcaacg acctgttcgc ggacggccgc gacgtatcct ggctgtggga
     4261 tgtcgatttc gagatattgt cggccactct cgacctcgtg ggcagggtac aggtgtccgg
     4321 catcagggcg ggcgatatgg cggtgcgcct caagtatgcc ggtgtggacc ccacgctaat
     4381 caagttggac gtggatatag ccggtgcgct ggagaatgcg gttcgcgccc tgcctgcggg
     4441 ggagactctg tacgtcctac ctacatatac ggcgatgctg gccttccgca agatgctgta
     4501 cgaaaagggc tgggtgcaca gccagttctg ggagcaatag gttgtgacga gtgacgagtg
     4561 acaagcggac cgtggacggg ctgtcatgaa ctatcagcag tgggaagaca cagtgcctgc
     4621 ggagatcaag ggggatggtc ctctggactg tggaggtcta tcggctagct ctctttgctt
     4681 gtgcaatagg gtggaaagat gtaactcgac tagtaggtga tgcccgtaca cgatcgttgg
     4741 ctgatc
//
LOCUS       DASRWZ010000028         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_824145, whole genome shotgun sequence.
ACCESSION   DASRWZ010000028 DASRWZ010000000
VERSION     DASRWZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824145"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..1743
                     /locus_tag="VFH60_00380"
     CDS             319..1743
                     /locus_tag="VFH60_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6312262.1"
                     /translation="MNNASAENPHARPGTATLDAGQLEGSLAVDVVLPGHAEYEVARR
                     SWNRAFDCHPAAIVRCRTTGDVVEAVRFAVGNGLPVGVRSGGHSASGLCVVEGGLVID
                     LSLMNGMTIDPERRVATVGPGLTWGAFTKQANEYGLATPAGDSGSVGVGGLTLGGGIG
                     WLVRKHGLTIDHLLSAEVVTAGGQVLTASETEHPDLYWAIRGGGGNFGIATSFEFRLV
                     PVGLVFAGLVMFQAHPDLLASYLRVAAEAPEELSTIAMLTLGPPMPMLPPQYHGNPVF
                     LVSFCYAGNPQEGERVVAPLRALGSPVMQMVQPMPYPVLYEFSAEGTASRPASIRSLF
                     LDGLDAATATALVEHLDHATSHECMVQLRVLGGAMSRVPADSTAFGHRDKPYMATIIN
                     SWREAPGEAGEVEKHRAWTEAAWHMLKPHAAGAYVSFLGDEGDEAVREAYPPDTYARL
                     AAIKQRYDPTNLFRGNRNIPPVSG"
     gene            complement(1948..>2624)
                     /locus_tag="VFH60_00385"
     CDS             complement(1948..>2624)
                     /locus_tag="VFH60_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312263.1"
                     /translation="ATMAGLSYARDYFTQAEIQEFLDESSSSDSSVLNSAPAYLRESL
                     YFPYDAGADFVNELLAMDGFRAVDIALADPPASTEQILHPDKYFDSPRDEPIQVDLKP
                     LTDTLGAGWTMKDWGTLGEFDLRVILDENGAADPDEAAAGWGGGSYAFYRNGDMILMY
                     TAVEWDDQNEADEYFEAQEETFDNATRDGDLYEQDGRFYSLTRSGKTVTLISSNDRTA
                     VENAAK"
BASE COUNT          470 a          812 c          845 g          497 t
ORIGIN      
        1 cctgattaac cagttaatct tcataagcca cgggctgcac atgcaaagtc tccctttggg
       61 gacaaattca tccggtaagg tctattcaga gggtcaaaaa cgaaccagtc tgccgcaggg
      121 cagactttgt aacgtgtagc ccgcgatttc aatcgccggg cgtctcctgg cggctatgct
      181 gctttcctca tctgcctcac cggtgggtca gtcttactgc ccagagtgta ggtcctgcat
      241 ttagactttg caggctcttg acggcaccgc aagcaatgcg ctacaacata tccaatctag
      301 aatcgaggag acggcgcaat gaataatgct tccgccgaaa atccgcatgc acgccccggt
      361 accgcgaccc tggacgcagg gcaattagag ggcagtcttg ccgtggacgt ggttttaccc
      421 ggccacgctg aatacgaagt agcgcgcagg tcgtggaata gggcgttcga ctgccacccc
      481 gccgcgattg tgcgctgccg cactacggga gatgtagtcg aggcggtgcg cttcgcggtc
      541 gggaacgggc tgccggtggg cgtccgtagc ggggggcata gcgcgtcggg cctttgcgtg
      601 gtcgagggag ggctggtcat cgacctttcg ctgatgaacg gcatgaccat cgacccggag
      661 cggcgcgtcg ccactgtggg accgggcctc acctggggcg cttttacgaa gcaagctaac
      721 gaatacggcc tggcaacccc tgcgggcgat agcggctcgg tgggcgtggg tggcctcact
      781 ttgggcggcg ggatcggctg gctggtgcgc aaacatgggc tgaccatcga ccacctgctc
      841 tccgccgagg tcgtcaccgc cggcggccag gtgctcaccg cgagcgagac ggagcaccct
      901 gacctctact gggcgatacg cggcggcggc ggaaacttcg gcatcgccac ctcgttcgag
      961 ttccgcctcg tgccggtggg cctcgtgttt gccgggctgg tgatgttcca ggcccacccc
     1021 gatttgctcg cttcttactt gcgtgtcgcc gccgaagcgc cggaagagct aagcacaata
     1081 gcgatgctga cgctcggccc gcctatgccc atgctgccac cccagtacca cggcaatccg
     1141 gtgttcctgg tctcgttctg ctacgcgggc aatccccagg agggcgaacg ggtggtagca
     1201 ccgttgcgcg cgctcggctc gcccgtgatg cagatggtcc agcccatgcc ttacccggtc
     1261 ctctatgagt tctcggcgga aggcacggcc agccgcccgg cgagcataag gtcgctcttc
     1321 ctggatggcc tggacgcggc aaccgccacg gcgctggtgg agcacctgga ccacgcgacc
     1381 tcccacgagt gcatggtcca actacgcgtg ttgggtggcg ctatgtctcg cgtgcccgcc
     1441 gatagcaccg ccttcgggca tcgagacaaa ccgtacatgg cgacgatcat caactcctgg
     1501 cgggaggccc ccggcgaggc gggcgaggtc gaaaagcaca gggcgtggac agaagcggcc
     1561 tggcacatgc tcaagcctca cgccgctggg gcctatgtga gcttcctggg cgacgagggt
     1621 gacgaagcgg tgagggaagc ttacccgccc gatacgtacg cgcggctggc agcaataaaa
     1681 cagcgctacg accccaccaa tctgttcagg ggcaaccgca atatcccgcc ggttagtggc
     1741 tagtggctag tgatcagtgg tcagtggtta gtggaagacg ccctgtgggg acgccctgtg
     1801 gctaaagcca cgggctgaga agaggcaaag tcccctgcgg ggactaagca acatgagggt
     1861 agtctccgaa ggagacttcg ctacttccca gcccctggct tcagccacag ggcgtcatgc
     1921 agggcgtctt ccactaacca ctgaccacta cttagccgcg ttctccactg ccgtgcggtc
     1981 gttggacgag atgagcgtga ccgtcttgcc ggagcgggtg aggctgtaga agcggccatc
     2041 ctgctcatac aggtcgccgt ccctggtggc gttgtcgaag gtctcttcct gggcctcaaa
     2101 gtactcgtct gcctcgttct ggtcgtccca ctcgacggcg gtgtacatga ggatcatgtc
     2161 gccgttgcgg tagaaggcat agctgccccc accccagccc gcggcggcct cgtcgggatc
     2221 ggcggcgccg ttttcatcga gtatcacccg gaggtcaaac tcgcccagcg tgccccagtc
     2281 cttcatcgtc cagcccgcgc ccagcgtgtc ggtgagcggc ttcaggtcca cctgaatagg
     2341 ctcgtcgcgt ggggagtcga agtacttgtc ggggtgcagg atctgctcgg tggaggcagg
     2401 cgggtcggcc agcgcgatat ccacggctcg gaagccgtcc atcgccagca gttcgttcac
     2461 gaaatcggcc cccgcgtcgt aggggaagta caggctctct cgcaggtaag cgggagcgct
     2521 gttcagcacc gacgagtcgg aggaggagct ttcatcgagg aactcctgta tctccgcctg
     2581 cgtaaaatag tcgcgtgcgt aactcaggcc cgccatggtg gcat
//
LOCUS       DASRWZ010000029         5168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_824190, whole genome shotgun sequence.
ACCESSION   DASRWZ010000029 DASRWZ010000000
VERSION     DASRWZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824190"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2263)
                     /locus_tag="VFH60_00390"
     CDS             complement(<1..2263)
                     /locus_tag="VFH60_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312264.1"
                     /translation="MRVTLRPRTAAWVLCVLLLASGTAQNNPLSYAQTSSRHFPQTGY
                     TVQGRFLEYWQQNGGLAQQGYPISSELREVSTVDGKVYTVQYFERAVFEHHPENQPPY
                     DVLLSLLGVFEYERKYPQGAPGEVPNNSPDSVLFPQTGKRLGGRFLGYWQTHGALAQQ
                     GYPISNEFTEQSDLNGKTYTVQYFERAVFESHPENAGTPYEVLLSQLGTFHFRDMYLY
                     PPPVRPLPLNRVQRNPMLSTEYLVWNEATRPPGGHDPVSGTGEIWAMDLKTGKQFSVS
                     NDAPGDQWVQAISGSTIVWTDNSHTSSIYEFDVMAKDLATGATYTVASGPTDQVQPVI
                     NGDVVAWVESAGNTQRLLVKELTDDRIIGIASARLDTGMTIQNPKLSEDYLVWGEIFN
                     TGAFYPPKESYLKALNRKTGAAKVVATFRTGDRVGYPPQYSLADHRLVVTPFSGLDAK
                     TTIYDQDTGQSAPIKTEGFPDSPALLSTAISGDTLVWAGGPEIWGVNLNDGKAVPLVA
                     ARGDQELPAVAGDWLAWTNRSGPNDGLIGLTSLSGSFATAEARRGLVTVFDAEAARRP
                     VQYSSIDMVSPDEGWAVGNFGLLSHYMNGTWERVETNDMAPWHDVNMVSRDEGWAGTT
                     AGLMRYDGTNWVPVRDPAIPDVTIFGIDMLSPDEGWAVGAGAILHYKDGRWQREPMPG
                     ADPTTDLLFDLDMVSPDEGWAVGNYSVIWHYSGGKWTQYNEPTDSSLESLYMFSAEEG
                     WAAGTTGAILHYTN"
     gene            complement(2626..3420)
                     /locus_tag="VFH60_00395"
     CDS             complement(2626..3420)
                     /locus_tag="VFH60_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312265.1"
                     /translation="MKTKLSGYLALPMLLLVLLAGCDAGPPPSPTPTPKVPLEPGAAF
                     IMKMVPLDADQAVTSDVQEQARRVIEARAREMGVANPVVRAEEPDLLIVELRGQDNTE
                     DNSRELTRNGLLEIVDGGDVPLLEGTMVVTSEGGPTFEEGTPQGLKTYQTLITGREIV
                     QDKVTYKTNEFTGRPEVNFALNEEGARVFADFTANNITKYMPILLDKQVISSPQIQGA
                     IPSGEGIITGLTEDEARRLVAILRAGALPTRLELVETQQLGTATNP"
     gene            complement(3475..4557)
                     /locus_tag="VFH60_00400"
     CDS             complement(3475..4557)
                     /locus_tag="VFH60_00400"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HET6312266.1"
                     /translation="MADMDIARLRLHNQGIEGARFDRPEDVVGWLGAVQAQDYPAAKW
                     ALGLRLRDARDADVERAFADGAILRTHVMRPTWHFVAPADIRWLLALTAPRVHAASAY
                     QYRKLELDDATFSHSHDVLAKALEGGKQLTRPELGSALQQAGIDTHDLLRLTYIIMHA
                     ELEGIVCSGAKRGKQFTYALLDERAPASKPLTRDEALAELTLRYFTSHGPATLKDFVW
                     WSGLTATDAKAGLEAVKPQLTHGTKDGLTYWFAQSTPFAQAPTPTAHLFPNYDEYGVG
                     YTDRSALVDPTRPGSPDPQDSFFYLGNLIVVEGKAVGSWKRTLAKHTVALTTQTFTPL
                     TPVETAAITVAAQAYGAFLDMPVVLA"
     gene            complement(4615..>5168)
                     /locus_tag="VFH60_00405"
     CDS             complement(4615..>5168)
                     /locus_tag="VFH60_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007140764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HET6312267.1"
                     /translation="QDTFDEAAWLADIEAAREQAADYYLNGFNWRGSPVPEGFQGPLY
                     FPPDPKWRVEATLDRDAPGTGLRVQLPTSIGDLRDFDVYGTFRFTIDGQEHRLTAYRM
                     VPEHPDYDELFLPFKDATTGHESYGSGRYLDVPRHDASHYILDFNWAYNPSCAYSPRY
                     NCPYPPPQNTLAVRVEAGEKAPH"
BASE COUNT          858 a         1489 c         1764 g         1057 t
ORIGIN      
        1 cgttggtgta gtgcagtatc gcgccggtgg tgcctgccgc ccagccttcc tcggcggaga
       61 acatatacag gctctccagg ctggaatcgg tcggctcgtt gtattgggtc cacttgccgc
      121 cgctgtagtg ccaaataacg ctgtagttgc ctaccgccca gccttcatcg ggcgacacca
      181 tgtcaaggtc aaacagcaaa tcggtggtgg ggtccgcgcc gggcataggc tcgcgctgcc
      241 agcggccatc cttgtagtgc aggatggccc ccgcgccaac cgcccagcct tcgtcgggag
      301 atagcatatc gatgccgaag atggtcacgt cgggtatggc ggggtcgcgc acgggcaccc
      361 agttggtgcc gtcgtagcgc atcaggcccg ccgtggtgcc ggcccagccc tcgtcgcgcg
      421 acaccatgtt cacgtcgtgc cagggtgcca tgtcgttggt ctctacgcgc tcccacgtgc
      481 cgttcatata gtgcgacaac agcccgaagt tgccgacggc ccagccttca tcgggggaga
      541 ccatgtctat ggaggagtac tggaccgggc ggcgcgcggc ctccgcatcg aagacggtca
      601 ccaacccacg ccgcgcctct gcggtggcga acgacccgga cagggaggta agccctatga
      661 ggccatcgtt ggggccgctc ctattggtcc acgccagcca gtcaccggcg acggcgggaa
      721 gctcctggtc gccccttgcc gctaccaggg gcaccgcctt gccgtcgttc aggttgacac
      781 cccagatttc ggggccaccc gcccagacca gggtgtcacc cgatatagcc gtggacagaa
      841 gcgcaggaga gtcggggaag ccttcggtct tgattggggc ggactgcccg gtgtcctggt
      901 cgtagattgt agtctttgcg tcaagcccgc tgaatggagt gaccaccagc ctgtggtccg
      961 cgaggctata ctggggaggg tacccgaccc tgtcgccggt acggaaagtg gcgacgacct
     1021 ttgccgcgcc agtcttgcga tttagagctt tcaggtaaga ctccttgggt ggatagaaag
     1081 caccggtgtt gaagatttcg ccccacacca ggtagtcctc gctgagcttc gggttctgta
     1141 tggtcatgcc ggtatccagc cgggcggacg ctatcccgat gattctgtcg tcggtaagct
     1201 ccttcacgag cagcctttga gtgttgcccg cggactcgac ccaggcgacc acgtcgccgt
     1261 tgatgacggg ctgcacctgg tctgtggggc cgctcgccac cgtgtaagtc gcgcccgttg
     1321 ccaggtcttt agccatgacg tcgaactcgt agatggagga ggtgtggctg ttatcggtcc
     1381 agacgatagt ggagccggag atagcctgca cccactggtc gcccggggca tcgttggaca
     1441 cgctgaattg cttgccggtc ttgaggtcca tagcccagat ttcgcccgtg ccgctgacgg
     1501 ggtcgtggcc gccggggggc cgggtagcct cgttccagac aaggtactcg gtcgagagca
     1561 tggggttgcg ctgcacgcga ttgaggggga ggggccgaac ggggggcggg tagaggtaca
     1621 tgtctctaaa gtggaaggtg cctagctggc tcaggagcac ctcgtagggg gtgcccgcgt
     1681 tctcagggtg cgactcgaac acggcacgct cgaagtattg cacggtgtaa gtcttgccgt
     1741 tgagatcgct ctgctcggtg aactcgttgg agatagggta gccctgctga gcgagggcgc
     1801 cgtgcgtctg ccagtaaccc aggaacctgc cacccagccg cttgcctgtc tgggggaaga
     1861 gcacgctgtc ggggctgttg ttgggcacct cgcctggcgc accctgtggg tacttgcgct
     1921 cgtattcaaa gacgccaagc aggctgagca gcacgtcgta agggggctgg ttctcagggt
     1981 ggtgctcgaa gacggcgcgc tcgaagtact gcacggtgta gaccttgccg tctacagtgg
     2041 agacttcgcg cagctccgag ctgataggat agccttgctg ggccaggccg ccgttctgct
     2101 gccagtactc caggaagcgg ccctggacgg tgtagcctgt ctgggggaag tgccttgagc
     2161 tggtctgagc gtaggacaga gggttgttct gtgcggtgcc gctagccagg agcagcacgc
     2221 aaagcaccca cgcggcggtg cggggcctca aagtgacacg cattgacttt cctccgggtt
     2281 tcgagtgggc gtcgtccgtg gaatacgctt gcacataaaa cggaccgggg gaataggggt
     2341 tgcgctgcgg ctgtgtggag gctagcggga ctctattgcc cctgtcatcc tgagcccttc
     2401 ggctacgctc agggtaaact ccgcgaagga tcagccgccc gcgcttgact cttggtactg
     2461 aagtaccagt gttcctgatg ccgccttgca tccccaacgt tgtagaccct ggcctaccca
     2521 cctgatcctt cgctacgctc aggatgacaa gggggactac aggctgacag gagggcgcag
     2581 ggttccagtg cctatgtcgg ggcaaccgtg ggattgtgca aggggctagg ggttggtggc
     2641 ggtgccaagc tgctgcgttt ctacgagttc cagcctggtg ggtagggcac ctgccctcaa
     2701 tattgccacc aaccgcctgg cttcatcttc agtcagaccc gttatgatgc cttctcctga
     2761 cgggattgcg ccttgtatct ggggagagct gattacttgc ttgtccagca ggatgggcat
     2821 gtacttggtt atgttgttag ccgtgaagtc ggcgaaaacg cgggcaccct cctcgttgag
     2881 agcaaagttc acctcaggtc ggcccgtgaa ctcgttagtc ttgtaggtga ctttgtcttg
     2941 cactatctcc ctgccggtaa tgagagtctg gtacgtcttc aagccctggg gggtgccttc
     3001 ctcgaaggtg ggccctcctt cgctggtgac taccatcgtg ccctccagca gaggtacgtc
     3061 cccgccatcg acaatctcca ggaggccgtt gcgcgtaagc tcgcggctgt tatcctcggt
     3121 gttgtcctgg ccgcgcaact ccacgattag caggtcaggc tcctcggcgc gcaccacggg
     3181 gtttgcgacg cccatctccc tggcgcgggc ctcgattacg cggcgtgcct gctcctgcac
     3241 gtcggacgtt acggcctggt ccgcgtcgag cggcaccatc ttcataatga aggcggcccc
     3301 aggctcaagc ggaactttgg gcgtgggcgt aggcgaggga ggcgggcctg cgtcacaccc
     3361 ggccagtaag actagcaaga gcatgggcaa cgcaaggtag ccggacaact tcgttttcac
     3421 aagaggctcc tctcggattt tagattttgg gttttggatt tgttgttcgg gaagttacgc
     3481 taggactact ggcatatcaa ggaacgcgcc ataggcttgg gcggctacgg tgatggcggc
     3541 ggtttcgacg ggggtgaggg gggtgaaggt ttgggtggtg agggctacgg tgtgtttggc
     3601 gagcgtgcgc ttccaggagc ctactgcctt gccttctacg acgatcaagt tgcctagata
     3661 gaagaagctg tcctgggggt cggggctgcc gggtcgcgtg gggtctacca gggcgctgcg
     3721 gtctgtgtag cccacgccat actcatcgta gtttgggaat aaatgcgcgg tgggtgttgg
     3781 ggcttgtgcg aatggggtag attgggcgaa ccagtaggtc aggccgtcct tggttccgtg
     3841 ggtgagttgg ggtttgaccg cttccaggcc ggctttggcg tcggtggcgg tgaggccgga
     3901 ccaccagaca aagtccttca gcgtggcggg gccgtggcta gtgaagtagc gtaaggtaag
     3961 ctcggccagg gcttcgtcgc gggtcagggg cttcgacgcc ggtgcccgct cgtcaagcag
     4021 ggcgtaggtg aactgcttgc cccgcttcgc gccgctgcac acgatgcctt ccagctcggc
     4081 gtgcatgatt atgtaggtca agcgcaacag gtcgtgggtg tcgataccgg cctgttgcag
     4141 tgcggaccca agctccggac gggtcagctg cttgccacct tccagggctt tcgcgagcac
     4201 gtcatggctg tgggagaagg tggcgtcgtc cagttcgagc ttgcggtact ggtaggcgct
     4261 ggcggcgtgg acgcgtgggg cggtgagggc gagcagccag cgtatgtcgg cgggcgcgac
     4321 aaaatgccag gtaggccgca tcacgtgggt gcgcaggata gccccgtccg cgaacgcccg
     4381 ctccacgtca gcgtctcgcg cgtcccgcaa ccgcaagccg agcgcccact tcgcggctgg
     4441 gtagtcctgg gcctgcacgg cacccagcca acccaccaca tcctccggcc tgtcaaacct
     4501 ggcaccctcg atgccctggt tatgcagccg caaacgcgca atatccatat ccgccatcac
     4561 cctggcttct cctcccaaaa tccgaagttc gaaattccaa tttccaaatc tcggtcagtg
     4621 cggcgctttt tcgcccgcct ctacccgcac cgcgagcgtg ttctggggcg gggggtaggg
     4681 gcagttgtag cgggggctgt aggcgcagga ggggttgtag gcccagttga agtcgaggat
     4741 gtagtgggag gcgtcgtggc ggggcacgtc caggtagcgg cccgagccgt agctttcgtg
     4801 cccggtggtg gcgtccttga aggggaggaa aagctcgtcg taatcgggat gttcgggcac
     4861 catgcggtag gcggtgagcc ggtgctcctg cccatcgatg gtgaagcgga aggtgccata
     4921 cacgtcgaag tcgcgcaggt cgcctatgga ggtggggagc tgcacgcgca ggccggtgcc
     4981 gggggcgtcg cggtccaacg tggcctcaac gcgccactta gggtcgggcg ggaagtaaag
     5041 cggcccctgg aagccctccg gtacggggga gcctcgccag ttgaagccgt tgaggtagta
     5101 gtcggcggcc tgctcgcggg cggcttcgat gtcggccaac catgcggctt cgtcaaacgt
     5161 gtcttgca
//
LOCUS       DASRWZ010000030         4654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_481595, whole genome shotgun sequence.
ACCESSION   DASRWZ010000030 DASRWZ010000000
VERSION     DASRWZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481595"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..3479
                     /locus_tag="VFH60_00410"
     CDS             39..3479
                     /locus_tag="VFH60_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HET6312268.1"
                     /translation="MYLRIAGSARTLAGDTLGRAYEFAYKVELHTPRLRLLDGRVATS
                     AQPVSVRYEALADSKGATLDGVKFDVYGFPSERLSALGAQSRAWPAPLPPGIMEGLAR
                     VEVPAPTLLAKGVADIGSLPAGIYLLVATVPYQSEVFADWQLLLVEDGSLALTGDGAA
                     FWATSSVGKAWEGAEISLYSPEGALLEKGLADHTGLWLPTSKGSGASLAVARDGAGHL
                     AALTLDPSSKWDNAPSGSLAVTMQTDLPAYRPGQPVNFRALLRQPRDTAVSTPVAEQQ
                     VSVFLLMPAGPVLSALTLKPDGAGGVSGLFNLSPDLKPGIYTLRVRAGDTGRDFPLRV
                     VAASDDVLSVYIAPGDSIEPGSATVTRTVSVLRAGGEPAAGAALTSSLGIEGDVWLAQ
                     PIRVAAGKDGRATIVASLPAWLVNYNEPALYLRVEAALSGQEGSDIQYLDAMSVRAAQ
                     AGVRQFVSPPMDVAVVASPQADRTTKVRIVSLGEVPLTGDLLALAESPAGERLAWALD
                     LAEKRDVTLDLPPRFGGGRMQFLRAGVRASRMLDLMPNQDPTLALNVDAPESVASGSQ
                     IPVRLGFAGEMDGAGKLSGTASVWLRRVSYPDGDEDSYLSWESGVKIDSSVPATATLT
                     APGHGLWYVMAEVGTDDGIYMQSWSVVRVMPGPSIQAPSMQKAPSGVTQNVSIVVHNP
                     GKEAISSGVRAVGGGSLRVLGGGSQPVEVAAGGWQRLNWTYVAASPQQAQIEFQFMPS
                     SGVEGVWTLDVQVEVNPRTVVTYTSGVFVGERLVGVQVPSGLSSDRVELEIRASTSLL
                     PALANIASGLPAGQIGQGDGADWAAARLSGASSVAAAYNRVGYGPAAIELSSVERSLA
                     LQQIYAAQHADGGWGVEGSDVESTAHVLLALRRQQISWTGAGGEAIPPADVEVISRGL
                     AYLAYEVGRPLTDRETQALMDERAYGLYALASYGQSDAEWARALMAYAGSGSGQVTLS
                     RAGQARLALALMQSGSTQDAFALVDRLLQARPASADTDVALAPLLELLVASGSTSRVG
                     AGATNKATYEAGASSYARSLMEARRGTGWSSPAATAGALWALSLYAKATGEKPVRGSP
                     SVTLHDRPVQAAVLPDDPGTISVVLPGDALQAGNNWLKLKAPAPDQSLYYSLTLRVTR
                     "
     gene            3632..>4654
                     /locus_tag="VFH60_00415"
     CDS             3632..>4654
                     /locus_tag="VFH60_00415"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET6312269.1"
                     /translation="MERLIVEGGYALHGEITPTGNKNSAQPMLAACLLTDEPVTLHNV
                     PDIADVRTLLNILRGMGVEVEASDLSTTHTITLQARVVDRQPDRSLGSQVRGSILMAG
                     PLLARTRYAVVGQPGGDAIGRRRIDTHLLALQALGATVEVDGDHYRMHAERLVGADIF
                     LDEASVTGTEQAILAAVLAEGQTTICNAAAEPHVQDLCHMLNGLGAQISGIGTNTLSI
                     QGVSSLHRGEHTILSDHMEVGSFIGLAAVTGGELRIRKAVPEHMRMSRLVFKKLGVEV
                     RVDGDDIVVPGGQDLKVAYDVHEAVPKIDDGIWPAFPSDLMSIALVTASQSEGTVLIF
                     EKMFENR"
BASE COUNT          841 a         1363 c         1553 g          897 t
ORIGIN      
        1 tcaccgtctc gccgtcagcg ccattaccgt tgggagaggt gtatctgcgt atagcgggga
       61 gcgcgcgcac gctggcgggc gacaccctgg ggcgagcata cgagttcgcc tacaaggtgg
      121 agctacacac gccccggctg agattgctgg atgggcgcgt ggcgacttcc gcgcagcccg
      181 ttagcgtgcg gtatgaggcg ctggctgata gcaagggcgc tacacttgac ggggtgaagt
      241 tcgacgttta tggcttcccc tcagagcgac tctcggctct gggcgcgcag tcgcgtgcct
      301 ggcccgcacc cctcccgccc ggcatcatgg aggggttggc gcgggttgaa gtgcctgccc
      361 ccacgctgct ggctaaaggt gttgccgata ttggcagcct cccggcgggc atttatctgc
      421 tggtggctac ggtgccctac caatcagagg tatttgccga ttggcaattg ctccttgtag
      481 aagacggcag cctcgcgctc acaggggatg gcgcggcgtt ctgggcaact tcgagcgtgg
      541 gtaaggcgtg ggaaggggcc gagatctccc tctattcgcc cgaaggcgcg ctgctggaaa
      601 agggcctcgc agaccatacc ggcctctggc tgcccacttc taaaggatcg ggtgcaagtc
      661 tcgcggtggc aagggacggg gcgggacatt tggctgcgct cacgttggac ccctcaagca
      721 aatgggacaa cgctccatcg ggcagcctgg cggtgactat gcagaccgac ctgcctgcgt
      781 accgtccggg ccagcccgtg aacttccgcg ctctcctgcg gcagccccgc gataccgccg
      841 tgtccacgcc cgtcgcggag cagcaggtgt cggtattctt gctcatgcct gccggacccg
      901 tcttatccgc gctcactctc aagcccgatg gagcgggcgg ggtgagcggc ctatttaacc
      961 tgtcgcccga cctcaagcct ggcatctaca cgttgcgcgt gcgtgcgggc gatacagggc
     1021 gcgattttcc attacgtgta gtagccgcgt ctgatgacgt tttaagcgta tatatagcgc
     1081 cgggcgacag catcgaaccc ggctccgcaa ccgtaacgcg cacggtgagt gtgttgcgcg
     1141 ccggtgggga gcctgccgcc ggggccgccc tgacgtcttc cctaggcata gagggtgacg
     1201 tatggctagc tcagcctatc cgggtggcgg cgggcaagga tgggcgggct acgattgtcg
     1261 cgtcgcttcc cgcgtggctc gtcaattaca atgagcctgc cctgtatctt cgcgtagaag
     1321 ctgcattgtc ggggcaagag ggtagcgata tacaatacct ggacgccatg tccgtgcgcg
     1381 ccgctcaagc tggggtgcgg cagtttgtct cgcctcccat ggacgtggcg gtggttgcca
     1441 gcccacaagc agaccgcaca acaaaggtgc gcatagtcag cctgggggaa gtcccactga
     1501 caggcgacct gctggcgctt gccgaatcgc ctgcgggcga gaggcttgcc tgggctttgg
     1561 acctggctga gaagcgtgac gttacgttgg atcttccgcc gcgcttcggt ggcgggcgca
     1621 tgcagtttct ccgagcgggt gtgcgtgcct ctcgcatgct cgacctgatg cccaaccagg
     1681 acccgaccct cgcgctgaat gtagacgcgc ctgagagcgt cgcatccggc tcgcagatac
     1741 ctgtcaggtt gggctttgcg ggcgagatgg acggtgcggg caagctatcc ggcacggctt
     1801 cggtgtggct caggcgggtg tcttacccag atggcgatga ggactcgtac cttagttggg
     1861 agtcgggcgt caaaatcgac tcgtccgtgc ctgccactgc cacgttaact gctcccggcc
     1921 acggcctatg gtacgtcatg gccgaggtcg gcacggacga cggcatctat atgcagtcat
     1981 ggtcggtagt cagagtcatg cccggaccct ccatacaggc accctcgatg cagaaagcgc
     2041 cgtccggcgt gactcagaac gtgtctatcg tcgttcataa tcccggcaaa gaggctatct
     2101 cctccggcgt gcgcgcggtt ggcggcggct cgctgagggt gctgggcggc ggctcgcagc
     2161 ctgtagaagt ggctgccggg gggtggcaaa ggctgaattg gacctacgta gcggcctcac
     2221 ctcagcaagc acagattgag ttccaattca tgccaagttc aggcgtggag ggagtctgga
     2281 cgttggacgt gcaggtagaa gtgaaccccc gcaccgttgt tacttatacc tccggcgtgt
     2341 tcgttggaga gcgattggtg ggggtgcagg tgccttccgg cctgtcgagt gatagagtag
     2401 agcttgagat ccgcgcttcg acttcgctgc tgcctgccct ggccaatatc gcgtccggcc
     2461 tcccggcggg gcaaattgga cagggcgatg gggcagattg ggcggcggct cgcctgagtg
     2521 gcgcgtcctc ggtggctgct gcctacaacc gggtggggta cggtccggct gcgatagagc
     2581 tatccagcgt ggagcgttcg ctggccctgc aacaaatcta cgcggcccag cacgcggacg
     2641 gaggctgggg tgtggagggt agcgatgtag agagcacggc ccacgtgctg ctcgccctgc
     2701 gccgccagca aatctcgtgg acaggcgcgg gcggcgaggc cataccccct gcggacgttg
     2761 aagtgataag ccgtggcctg gcatatctcg cctatgaggt tggcaggccg ctaactgacc
     2821 gggaaaccca ggcactcatg gacgagcgtg cttatggcct gtatgccctc gcatcttacg
     2881 ggcaatctga cgctgagtgg gcgcgtgcgc tcatggcata tgcgggttca ggtagcgggc
     2941 aagttacgct ctcgcgggcg ggtcaggcga ggttagcact ggcgcttatg cagtcgggca
     3001 gcacgcagga cgcttttgcc ctcgtggaca ggctgcttca ggcacggcca gcctcggcgg
     3061 atacggacgt tgccctcgcg cccctgctgg aactcctggt cgcaagcggc tctaccagcc
     3121 gggtcggagc aggggctaca aataaagcaa cttatgaagc gggtgcctca tcatatgccc
     3181 gttcgctcat ggaggcgcgg agggggacag gctggtccag ccctgccgcc accgcaggcg
     3241 ctttgtgggc gctctctcta tatgctaagg ccacagggga aaaacctgtg cgtggctcgc
     3301 ccagcgttac cctgcatgac cgcccggtgc aagccgcagt cctaccagac gacccaggca
     3361 ctatctccgt agtgctgccg ggcgatgcct tgcaagcagg gaacaactgg ctcaagttga
     3421 aggcccccgc gccggaccaa tcgctgtatt acagcctcac gctgcgtgtg acgaggtaga
     3481 acgtattgcg tattgcgtat tgcgtattac gtagtacgta aagcacaacc ctcaaacaaa
     3541 accttagcga acttagtgct ctttgtgtct ttgtggttta agaattacgc aatacgttct
     3601 acgcaatacg attcaggcag tcaggagata gatggagcga cttatagttg agggtgggta
     3661 tgccctgcat ggggagataa cgcccaccgg gaacaagaac tcggcccagc ctatgcttgc
     3721 ggcttgcctg cttacggacg agcctgtgac gctccataac gtgccggaca tagcggacgt
     3781 gcgtactctg ctcaacatcc tgcgtggcat gggcgtggag gtcgaagcgt cggacctttc
     3841 taccacccat accatcacgt tgcaggctcg cgtcgtagac aggcagcccg accgcagcct
     3901 gggatcgcag gtcagggggt ctatcctgat ggcggggccg ttgctggcgc gaacaaggta
     3961 cgcagtagtg ggccagccgg gcggcgacgc tattggccgc aggcgcattg acactcactt
     4021 gctggccctg caagccctgg gagcgacggt tgaggtagac ggcgaccact acaggatgca
     4081 cgccgagcga ttggtaggcg cggacatatt ccttgacgag gcgagcgtga ccggcaccga
     4141 gcaggctatc ctggccgccg tgcttgccga aggccagacc actatctgca acgctgccgc
     4201 cgagccgcac gtccaggacc tgtgccacat gctgaacggg ttgggcgcgc agataagcgg
     4261 cataggcacg aacaccctgt ccattcaggg cgtgagtagc ctccacaggg gcgagcacac
     4321 tatcctttcg gaccacatgg aagtaggcag cttcattggg ctggcggcgg tcacgggcgg
     4381 ggagctacgc atacgcaagg cggtgcctga gcacatgagg atgagccggc tggtcttcaa
     4441 gaagctgggg gtcgaggtac gggttgacgg agatgatata gtcgtgccgg gcgggcagga
     4501 cctcaaggta gcgtatgacg tgcatgaggc cgtgccgaag atagacgacg gcatatggcc
     4561 cgccttcccc tccgacctga tgagtatagc ccttgtcacg gcctcccaat ccgaaggcac
     4621 ggtgctgata ttcgagaaga tgttcgagaa cagg
//
LOCUS       DASRWZ010000031         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_276048, whole genome shotgun sequence.
ACCESSION   DASRWZ010000031 DASRWZ010000000
VERSION     DASRWZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276048"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..608
                     /locus_tag="VFH60_00420"
     CDS             360..608
                     /locus_tag="VFH60_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312270.1"
                     /translation="MRVLCAKDAGGAPGERRPLEGFLRGKPRRPRGEAGEAREAGEQG
                     GEGTAGARDRQPFGFYRPDPEDEERDRVARRLWKQVFG"
     gene            950..1222
                     /locus_tag="VFH60_00425"
     CDS             950..1222
                     /locus_tag="VFH60_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312271.1"
                     /translation="MTDTGVDEQVMLAVRECTHQAARFHGEDEHGPSGVPVGAACVVA
                     ECGLSVDVTLQALERLTAAGLIYGIGAIEHGGEPHYILAPHLDDGV"
     gene            1226..2413
                     /locus_tag="VFH60_00430"
     CDS             1226..2413
                     /locus_tag="VFH60_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET6312272.1"
                     /translation="MDSDRLGQLQQALRNRGLDGWLFYDFRQSNPIAYRVLGLSTAQL
                     FTRRWYYWLPAEGEPSRLVSAIEAGNLDSLPGRKILYRSRQQLAEGLAQMLAGHTTVA
                     MEYSPLAEIPVVSRVDAGTVEYIRGMGKTVVSSADLVQAFEAPLSEHAFRTHLEAERR
                     LVAIHAATLDFIRENVSAGHTLTEYVVQQHMLGLYEQQGLFRDHGPDVAVGPNASDPH
                     YAPTEGQSLPLREGDLLLIDWWGKLPEEGAVYADYTWVGVVGSSVPERYRAIFDVVRG
                     ARDAALEFIERHVRSGTEIEGWQVDDIARAYVTERGYGDYFIHRTGHNLGVDLHGPGA
                     NLDNLESHDTRQLLPHTLCTVEPGIYLPEFGVRSEVNVYIGDGTIEVTGRPIQQELPA
                     ILA"
BASE COUNT          519 a          762 c          886 g          485 t
ORIGIN      
        1 gagccactat gtccatagac gaaggtcgtg cggggttgcg cggcaccggc agggaggatg
       61 tgccgctgcc ccggcatcgc acacgcgcgg caagctgtgg agcatccggc gtcccctgca
      121 gagaggctag taggcgcatc aagaagcata cctccctttc tgtaacgtgg taaatgacga
      181 aaaggtacgg ctggggggcc gtacctttcg cccggctgga gtgggtggct tggctttacc
      241 ctaaagtggt agtaaaatcg aggcatagga ggaacgccgc cttggacatt ctgcgacgcg
      301 gacgggcgcg gcagggcggc agggtggcat tttcagcggt aggcaagtgg agagcgagca
      361 tgcgagtctt gtgtgctaaa gacgccgggg gcgctcccgg cgagcggcgg ccgttggagg
      421 gcttccttcg aggcaagccc cgcaggccga ggggagaggc tggggaggcc agggaggccg
      481 gtgagcaggg cggcgaaggt acagcaggtg ccagggaccg gcagcctttc ggtttctaca
      541 ggcccgaccc ggaagacgag gagcgagacc gggtggcgcg acggctgtgg aagcaagtct
      601 ttggataatt cacagccgcc tggtcgcaag gtgggaccta tatgcaaagt gccgagtgct
      661 atgagtgcgg gaccaaagtc tcccctgtta ttccgagcgc agcgaagaat ctaaaatgcc
      721 gctgcacaaa ggcggttgaa cattacgtac gatggtgctg actttcagca tagaggcaaa
      781 gcctgcaact tagattcttc gctacgctca gaatgacaag gaccgcctct ctccgcagat
      841 tggcactgcc atcaattgct cactgcagta gcggacctga ccatctgagt gtgacccatc
      901 aagttgggcg gtcctctata atggaagagg cagtgtgtcg gagcgggttg tgacagacac
      961 cggcgtggac gagcaggtca tgctggcggt acgtgagtgc actcaccagg cggcccgctt
     1021 tcatggagaa gacgagcacg gcccgtcggg cgtgccggtc ggggctgcgt gcgtcgtcgc
     1081 tgagtgcggg ttatctgtgg acgtgacgtt gcaagctctt gagcggctga cagcagccgg
     1141 gctgatatat ggtatcggtg cgatagaaca cgggggcgag cctcactaca tacttgctcc
     1201 acaccttgac gatggagtgt gatacatgga ctctgatcgc ttgggacagc tacaacaggc
     1261 gctacgcaac cgtggcctgg acggctggct cttctacgac tttcggcagt cgaaccctat
     1321 agcttacagg gtgctgggcc tctccacggc gcagcttttc acccggcgct ggtattactg
     1381 gctacccgcc gagggcgagc cgtcccggct ggtcagcgcg attgaggcgg gcaacctgga
     1441 cagcctgccg gggcgtaaga ttctctatcg cagccggcag cagttggccg agggcctggc
     1501 tcagatgctg gccgggcata ccactgtcgc tatggagtac tcgcccctgg ccgagattcc
     1561 cgtggtgtcc agggtagacg caggcacggt ggagtacatc cggggcatgg gcaagacggt
     1621 ggttagctcc gccgacctgg tgcaggcgtt tgaggcaccc ctttccgagc acgccttccg
     1681 cacgcattta gaggccgaga ggcggctggt ggctatccac gccgctaccc tcgactttat
     1741 tcgcgagaat gtgtccgcgg gacacaccct gaccgagtac gtggtccagc agcacatgct
     1801 tggcctttac gagcagcagg gcctgtttcg ggaccacggc cccgacgtgg cggttggccc
     1861 caacgccagc gacccccact acgcccccac cgagggacag tcgctgccac tgcgcgaggg
     1921 cgacctgttg ctgattgact ggtggggcaa gctgcccgaa gagggggcgg tttacgccga
     1981 ctacacctgg gtaggcgtgg taggctccag cgtgcctgag cggtaccgcg ccatcttcga
     2041 cgtggtccgt ggggcgcggg acgcggcgtt ggagttcatc gagcggcacg tgcgctctgg
     2101 cacggagata gagggttggc aggtggacga tatcgcccgc gcctacgtca cggagcgggg
     2161 ctacggcgac tacttcatcc accgcacggg acacaacctc ggagtggacc tgcacgggcc
     2221 gggcgctaac ctggacaacc tggagtccca cgacacccgg cagctcctgc ctcacaccct
     2281 ctgcactgtg gagcctggca tctacctgcc cgagttcggg gtgcgtagcg aggtgaacgt
     2341 ctacatcggc gatggcacga tagaagtgac gggccgccca atccagcagg agctacccgc
     2401 tatactggct taggagcggt cgagcggagt cgcatcagcg ggatgcaacg agtagtcatt
     2461 tggagggacc acactccccc ctgtcatcct gagcgtagcg aaggatctaa gatgccgccg
     2521 ttgcctcagt gccgaacctt tagcaacatt acccctataa tcaacattgt tcccttacca
     2581 attactttag atccttcact gcgttcagga tgacaagggg aacagagtac cgcagtcagc
     2641 acacgcaggg ac
//
LOCUS       DASRWZ010000032         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_276143, whole genome shotgun sequence.
ACCESSION   DASRWZ010000032 DASRWZ010000000
VERSION     DASRWZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276143"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1912)
                     /locus_tag="VFH60_00435"
     CDS             complement(<1..1912)
                     /locus_tag="VFH60_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6312273.1"
                     /translation="MPNRLAAETSPYLLQHKDNPVDWNPWDREALERAKADDKPILLS
                     IGYSACHWCHVMEHESFENPEIAALMNEHFVSIKVDREERPDLDSVYMEAVQAMTGHG
                     GWPMTVFLTPDGVPFFGGTYFPPAPRHGMMSFPQVLIAVSRAYHEQRDKVVQSAQEMR
                     GFLQSSMAVEPARIEPTPSILDDAARNMLSQMDRVNGGTHGSPKFPQPMNLEFMLRAY
                     RRTGNIDLLALAELSLQKMALGGLYDQVGGGFHRYSVDDVWLVPHFEKMLYDNAQLAR
                     VYLAAFQVTGNGFYRRIAEETLDYVLREMTSGEGGFYSTQDADSEGEEGKFYVWTPQE
                     IVEVLGEEDGRVFNMLFDVTERGNFEGHNILHLSRPLQELAAQAQIEPDKLEDLVQRS
                     REHLYQAREQRVHPGRDEKILVGWNGLMLRAFSEAASILGRDDYRTAAIANAEFILST
                     MVQQAADDGGEIRLHRTYKDGRAHILAFAEDYAFYANGLVSLYEATFEPRWIAAARSL
                     VSTLMEHFWDGATGGFFSTSDFHEALVARPKEWYDNAIPSANAEAAEALFRLYLLTTE
                     PDYEQYALDTMRPLLDKLGRAPTAFGRMLCALDFYLGSPVEVALIGDMPSGDMQEMLR
                     AVWRPYAPNKVVA"
BASE COUNT          376 a          687 c          690 g          391 t
ORIGIN      
        1 cagccactac cttgttaggg gcatacggcc tccataccgc ccgtagcatc tcctgcatat
       61 cgcccgaggg catgtccccg ataagggcta cctccacggg cgagcccagg tagaagtcga
      121 gtgcgcacag catgcggccg aacgcggttg gcgccctgcc aagcttgtcc agcagcggac
      181 gcatggtgtc gagggcgtac tgctcgtagt ctggttcggt ggtcaggaga tacaggcgga
      241 acagagcctc ggcagcctct gcgttggccg acgggatagc gttgtcgtac cactccttgg
      301 gccgggccac cagcgcctcg tggaagtcgg aggtggagaa gaagcccccc gtcgccccgt
      361 cccagaagtg ttccatcaag gtggacacga gggagcgggc cgccgcgatc cagcgaggct
      421 cgaacgtggc ctcgtacagc gacacaagcc cgttcgcgta aaaggcgtag tcttcagcga
      481 aagccaggat gtgcgccctg ccgtccttgt aggtgcggtg gaggcggatt tcgccgccgt
      541 cgtccgcggc ctgctggacc atcgtggaca ggatgaactc ggcgttggca atggcggccg
      601 tccggtagtc gtcgcggccc aggatgctgg cggcctcgga gaaggcgcgc aacatgaggc
      661 cgttccagcc gaccagtatc ttctcgtcac ggccggggtg gacgcgctgc tcgcgggcct
      721 ggtaaaggtg ctcccggctg cgctgcacca ggtcttccag cttgtccggc tcgatctggg
      781 cttgcgcggc tagctcctgt agggggcgag acaggtgcag gatgttgtgt ccctcgaagt
      841 tcccgcgctc ggtcacgtcg aagagcatgt tgaacaccct gccgtcttcc tcacccagca
      901 cctcaactat ctcttgtggg gtccagacgt agaacttgcc ttcctcaccc tcgctgtcgg
      961 cgtcctgggt cgagtagaag ccgccctcgc cggacgtcat ctcccgcaat acgtagtcga
     1021 gggtttcttc cgcgatccgc ctgtagaagc cattgccagt cacctggaac gcagccaggt
     1081 agacgcgggc gagttgcgcg ttgtcgtaga gcatcttctc gaagtggggc accagccaca
     1141 cgtcatcgac cgaatagcgg tggaagccgc cgcctacctg gtcgtacagg ccgccgaggg
     1201 ccatcttctg caacgaaagt tcggccaggg ccagcagatc gatgttgccc gtgcggcggt
     1261 aggccctgag catgaactcc aggttcatcg gctgggggaa cttgggcgag ccgtgggtgc
     1321 cgccgttcac cctgtccatc tgggagagca tgttgcgagc ggcatcgtcc aggatggagg
     1381 gggtgggttc gatgcgggca ggctccaccg ccatgctgct ctgcaagaag ccccgcatct
     1441 cctgcgcgct ctggaccacc ttgtcgcgct gctcgtggta ggcccgcgag acggcgatca
     1501 gcacctgggg gaagctcatc atgccgtgcc gcggcgcggg cgggaagtag gtcccaccga
     1561 agaagggcac cccgtcgggc gtgagaaaga ccgtcatggg ccaaccgccg tgcccggtca
     1621 tcgcctgcac cgcctccatg tagaccgaat cgaggtcggg ccgctcttcg cggtccactt
     1681 tgatgcttac gaagtgctcg ttcatgaggg ccgcgatctc cgggttctcg aacgactcgt
     1741 gctccatgac gtggcaccag tggcaggccg agtagccgat gctcagtaga atcggcttgt
     1801 catcagcctt tgcgcgttcc agggcctccc ggtcccaggg gttccaatct acggggttgt
     1861 ccttgtgctg taacaggtag ggactcgtct ccgcggcaag tctatttggc atgcgtcaat
     1921 atcctcctgg gttaaggagt gagcaatctt cgagccaacg ccacgtcaga agaacaggct
     1981 ggctagagag gcgactccga ggcccgctat catcgcgccc gatgcccggt tcacccacac
     2041 cagcccgcgc ctgctcaccc gcgaccgcaa catgcccaca accgtgctga gtatgagcca
     2101 ccagcaggcc gagccgagaa acacccccag caccagcagc gccg
//
LOCUS       DASRWZ010000033         3348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1098525, whole genome shotgun sequence.
ACCESSION   DASRWZ010000033 DASRWZ010000000
VERSION     DASRWZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098525"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..2109)
                     /locus_tag="VFH60_00440"
     CDS             complement(295..2109)
                     /locus_tag="VFH60_00440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6312274.1"
                     /translation="MLDLRARQVMRFAPPLVLGLLLLAAFYLRASGHNWDQGHNLHPD
                     EPFVVRTGMQHVNWPQDVTLSALLDPARSPINVRGNGLVYSYGTFPLYSLRLVTVVAH
                     GLTGNDYFSEYFGVAQTGRVLTALFDALTVLAIFGVGARLWGRWIGLLCASLYAFAVL
                     PIQIAHIFGNEPYMALFVTLSLFCSVLVAQTGKRRYALLGGVAAGLALACKLSALPML
                     MLPLAGALVWCLKSGDRRGVRAVLQPRLFVMGALALAGAFLGLFLTDPYAVLDLPTYW
                     SQVQFQADIQRGAIDMVYTRQFIGTWPVLYPWLQVMLVGVNPIVGVAGTLGLVALVWR
                     VLRQRLLVEGLVLVGFVAYFAPVAISEARWMRYYVAAIPYLCLFAGAFVVWLWDLSSR
                     RGPAFPAQRVLAATAGLLVVSAMLGAAAYTSIYRTEHTEVQASRWMYDHVPRGSRVGA
                     MGDTVLPLPIDGKPPPDEAFSRTWYDLLGDKPSEIASREVRDFLRQLDYLTVPGIKVL
                     STVKPLPWRYPVQRRYHELLFSGQLGFEQIYKVTSYPRLFGLEVVDSQPPFEPNFVEY
                     DHHPVWVFRKVRDLSDAEWDALFAEAVKQPSIATRQTP"
     gene            complement(2302..3204)
                     /locus_tag="VFH60_00445"
     CDS             complement(2302..3204)
                     /locus_tag="VFH60_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET6312275.1"
                     /translation="MSEGPEIHRLSAQLTEELVGSRIVAIDTRLKKARTWLEMHPGML
                     EGREIERIYPAGKNLLWQVEGGVYFRFHLLMFGKVRTYTLRYRLPPDPRQRAHIVTTS
                     RQFALFNGQVFDIGTGDPFQQITTLRELGPDICAVPFDRDFFLQRLNRPDNLHREIGP
                     VLLDQTVAAGLGNYLKSDILFECGINPWTHVGDLTPEQQARLADVTPELSQRALRNRG
                     QTVTDEVMEVLLADPDNPNPRWRDRHWVFRQTNKPCKMCGFPIKQRRQGPDAGRITYF
                     CPNCQDVDLDQLSAAGVPEIKASS"
BASE COUNT          697 a         1009 c         1084 g          558 t
ORIGIN      
        1 cccctggcgc gagatagtcg aagagtcccg ctacctgtag cagctctaaa caccaagacg
       61 ccaaggacgc caaggaccac gccaagagta cgaaaacgca aagacgcaaa gaacgcaaag
      121 gacgcgcaaa gggtacgaag gtctagagtg tacaaagagg accagcgtac aacaccatgt
      181 accctggtcc ttttacttag aacacctatt taatcttggc gtggtccttg gcgtcttggc
      241 gtcttggcgg ttccgtctcc tgcaaattta ctgttgccgc aaagctgaca cgcgctacgg
      301 ggtctggcgg gtggcgatgg aaggctgctt caccgcttca gcgaacaggg cgtcccactc
      361 tgcatcgctc aggtcgcgca ctttgcggaa cacccacacc gggtggtggt cgtactcaac
      421 gaagttcggt tcgaacggag gctggctatc gaccacttca agcccgaaca agcgcgggta
      481 agaggtgacc ttgtaaatct gctcgaagcc aagttggccg ctgaacagca gctcgtggta
      541 gcgccgctgc acggggtagc gccagggtag gggcttgacc gtgctcaata ccttgatgcc
      601 gggcacggtc aggtagtcca actgcctcaa gaagtcgcgt acctcgcggg atgctatctc
      661 gcttggcttg tcgcccagca aatcgtacca ggtccgcgag aaggcttcat cgggcggggg
      721 cttgccatca atcggcagcg gcaacacggt atcacccatc gcgcccaccc tgctgcctct
      781 cggcacgtgg tcgtacatcc accgggaggc ttgcacctcc gtgtgctcgg tccggtatat
      841 cgacgtgtag gcggcggcac ccagcatggc ggatacgacc aggaggccgg cggttgcggc
      901 aagcacccgt tgtgcgggga atgccggtcc gcgcctcgac gagaggtccc acaaccacac
      961 cacgaacgcc cccgcgaaca ggcagaggta ggggatcgcc gccacgtaat agcgcatcca
     1021 ccgcgcctcg ctgattgcca ccggcgcgaa ataggctacg aagcccacca gcaccagccc
     1081 ttcaacgagc agccgctgtc tcagcacccg ccacaccagc gcgaccaggc ccagggtgcc
     1141 cgccaccccc acgatggggt ttacccccac cagcatcacc tgcagccagg ggtagagcac
     1201 gggccaggtg cctatgaact gcctcgtgta gaccatgtca atcgcgccgc gctgtatgtc
     1261 tgcctggaac tgcacctggg accagtaggt cggcagatcg agaacggcat aggggtcggt
     1321 gaggaacaag ccaaggaagg ccccggctaa ggccaaagcg cccatcacga acagacgcgg
     1381 ctgtaatacg gcacgcacac ctcgccggtc gccggacttc agacaccaca ccagcgctcc
     1441 cgccaggggc aacatgagca taggcagggc gctcagcttg caggcgaggg ccagtcctgc
     1501 ggcgacacca ccgaggaggg cgtagcggcg cttgcccgtc tgcgctacga gcacgctgca
     1561 aaacaaggag agggtgacga agagggccat gtaaggttcg ttgccgaata tatgggctat
     1621 ctgtatgggc agcacggcga aggcgtacag cgaagcgcag agcagcccta tccaccggcc
     1681 ccacagcctg gctcctaccc caaagatcgc cagcacggtg agcgcgtcaa aaagcgcggt
     1741 gagtaccctc ccggtctgcg ccactccgaa gtactcagag aagtagtcgt tgcccgtaag
     1801 gccgtgggcg acgaccgtta ccagccgcaa cgagtacaag ggaaatgtgc cgtagctgta
     1861 gacgagaccg ttgccgcgta cgttgattgg gctgcgggcc gggtccagca gggcactcag
     1921 ggtgacatcc tgcggccagt tgacgtgctg catgcccgtg cggaccacga aaggctcgtc
     1981 ggggtgcaag ttgtggccct ggtcccagtt gtggccggag gcgcgcaggt agaaggctgc
     2041 aagcagtagc aagcccagca ctagcggagg ggcgaagcgc atcacctgcc tggcacggag
     2101 gtcgagcatc cggtaaacct gcacaaaatg agttgacagc ctggtccagc gacgcgaagc
     2161 agtgccttat gacgggtgcg tgagtaggct tagtgcgccc cggcatgtgg catacctatg
     2221 gtacaccacg gtgtgcagca ggtccaaacc tgggtctatc tgtgcacaac cgctgtgcgc
     2281 gcacgaaggg cctacgagga gtcagcttga cgcctttatc tccggtacgc ctgccgccga
     2341 gagttgatcc aggtcaacgt cctggcaatt ggggcagaag taggtgatgc ggcccgcgtc
     2401 cggcccctgg cggcgctgct tgatggggaa gccgcacatt ttgcagggct tgttggtctg
     2461 gcggaagacc caatgccggt cgcgccagcg gggattgggg ttatcggggt cggccagcag
     2521 cacctccatc acctcgtcgg tgaccgtctg cccccggttg cgcagggcgc gctgagacag
     2581 ttccggcgtc acgtcggcaa ggcgggcttg ctgctcaggc gtcaggtcgc ccacgtgggt
     2641 ccaggggttg atgccgcact cgaacaggat gtcgctcttg aggtagttgc ccagacccgc
     2701 cgccacggtc tggtcgagca gcacggggcc tatctcccgg tggaggttgt cggggcggtt
     2761 gagacgctgg aggaagaagt cgcggtcgaa aggcacggcg cagatgtcgg ggccaagctc
     2821 gcgcagggtg gttatctgct ggaacgggtc gccggtgccg atgtcgaaca cctgcccgtt
     2881 gaacagggcg aactgccgag aggtggtgac gatgtgcgcc cgctgcctgg gatcgggagg
     2941 gaggcggtag cggagggtat acgtgcgcac cttgccgaac atgagcaagt ggaagcggaa
     3001 gtagacgcct ccctcgacct gccacaggag gttcttgccc gccgggtaga tgcgctcgat
     3061 ctcccgccct tcgagcatgc cggggtgcat ctccagccag gtgcgggcct tcttcaagcg
     3121 cgtatctatc gccacaatgc ggctgcctac caactcctcg gtgagctgcg ctgataggcg
     3181 gtgtatctca ggtccttccg acatgcaaaa caaccttgca aaatcggtac cagggttctg
     3241 ggacgtgagg agtaggagac ggaaccgcca agacgccaag gacgccaagg tagcaccaag
     3301 tatagaatgg tttagagaaa gcaaaaggac cagggtacat catgtcgt
//
LOCUS       DASRWZ010000034         4244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_756100, whole genome shotgun sequence.
ACCESSION   DASRWZ010000034 DASRWZ010000000
VERSION     DASRWZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756100"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2297
                     /locus_tag="VFH60_00450"
     CDS             <1..2297
                     /locus_tag="VFH60_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6312276.1"
                     /translation="LAVLALGSGGSSSAARAAATVGNNTNAIQGAGKIESKVLADTAN
                     GKTATFIVLMSERANLNSAYGMKDHDARGWYVYNTLRETAARTQADLRATLNARGIKY
                     KARWVANALLVTGDRSVVEMLAARADVRVIEASRPMYLIEPVRVGTDKSRASQSAPDS
                     PHDVEWGVASVRAPEVWAMGYTGQGIVVAGADTGIQWTHTALKSQYRGWNGATADHNY
                     NWWDAIDDEADPLDPCEPGSQEPCDGHSHGTHTVGTMVGDDGMGNQIGVAPGAEWMGC
                     RNMVSLGVGFGAVFDTYAECFEFFLAPTKLDGTAPDATKRPHVINNSWGCPEGCTPTT
                     LRDIIEASQASGILVEASAGNDGPACSTVTVPPAIHEASFTTGALDIHPEDGLFLADF
                     SSRGPVATDGSMRLKPNISAPGVDTRSAVIGESDAEYDEFSGTSMAGPHVVGVVALLW
                     SARPELARQITETKVLLQMSANPGVDVIEPIPMCGGTTPEDIPNNFFGWGAVDALAAV
                     QRAQGIEPTATAILPTATPGTPTATPTACPIQFTDVPADHTFYASVRCLSCRHIIGGY
                     PCGGINPDTGQAEPCTGNQPYYRPSSNVTRGQIAKIVAQSFEYNADPGEQIYADVKPG
                     DPFFTWINRLTAQDVMNGYPCGGPGEPCSNGSRPYFRPASYATRGQLSKIVANAAAFY
                     EFHNNQVFEDVPATQPFYMYIARLASRQIMTGYPCGGTNPDTGQAEPCGQGNKPYFRP
                     NSNVSRGQTAKIVANAAFPFCNTP"
     gene            2497..>4244
                     /locus_tag="VFH60_00455"
     CDS             2497..>4244
                     /locus_tag="VFH60_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6312277.1"
                     /translation="MSTREPTHALRPNIRVLMALALLVLAMMALGSGGSSSAARAAAP
                     TVPGANSAQPAGKIDPFVSQDLAGDGSASLVIRMADKADLRAAYGMKDHDARGWYVYN
                     TLRDHAARTQTGVRAFLDARGVSYESYWGANVIIAEGNRELVEVLATRTDVERIESNR
                     PFRGIDPIQSDPSDKSGANSPDGAIGIAWGVQNVRAPEVWAMGYRGAGIVIGNQDTGM
                     RWTHVAIKDQYRGWNGATANHNYNWWDGLRVPGDLPTNPCGYSTNEPCDDDELLGGGH
                     GTHTTGTVLGDDGMGNQVGVAPEATWMGCRNMERGVGRPQTYMECFEFFLAPHDLEGN
                     NPDPTKRPHVMNNSWICVEGCAPNTLQDLVEASQASCIFVEVSAGNDGPTCSTIQFPP
                     QIYEASFDTGSITVDEESGLPILSDFSSRGPVLSDGSMRIKPNVVAPGEDIPSALRGS
                     DTEYGNLDGTSMAGPHTVGVVALIWSARPELVRQITETKVLLQLSANPDVHVVDPIQE
                     CGGTTEADIPNNHFGYGRVDAFAALQRAQGIEPTATAILASPTPGTPTATPTACPIQF
                     ADVPANHTFYASVRCLS"
BASE COUNT          862 a         1434 c         1264 g          684 t
ORIGIN      
        1 tgctggctgt gctggcgcta gggtccggcg ggtcgagtag cgccgcgagg gcagcggcaa
       61 ctgtcggtaa taacacgaat gctatccagg gggccggcaa gatcgaatcg aaggtgctgg
      121 ccgataccgc caatggcaag accgcaactt tcatcgtgct catgtcggag cgcgccaacc
      181 ttaattcagc ctacggcatg aaagaccatg acgctcgcgg ctggtacgtc tataacaccc
      241 tcagagagac cgccgcccgc acgcaggcag atctacgcgc cacgctcaat gcgcggggca
      301 tcaaatacaa ggccaggtgg gtggctaacg ccctgctggt gacaggcgac cgctcggtag
      361 tggagatgct tgccgcccgc gccgacgtac gtgtaatcga ggctagccgg cctatgtact
      421 tgatcgagcc tgtaagggta gggacggaca aatcgcgcgc gtctcagtcc gcgccggaca
      481 gcccccacga cgtggaatgg ggcgtggcaa gcgtgagagc gccggaagta tgggcaatgg
      541 gctacacggg acaggggatc gtggtggccg gggccgatac cggtatacaa tggacgcaca
      601 cagctctcaa gtcacagtac cggggctgga acggcgcgac cgcggaccac aactataact
      661 ggtgggacgc catcgatgac gaagccgacc ccctcgaccc gtgcgagcct ggctcgcagg
      721 aaccgtgcga cggccacagc cacggcacgc ataccgtcgg caccatggtc ggtgacgacg
      781 gtatgggcaa ccagataggt gtagcccccg gcgcagagtg gatgggttgc cgcaacatgg
      841 tgtccctcgg cgtcggcttc ggggccgtgt tcgacaccta tgccgagtgc ttcgagttct
      901 tcctagcccc caccaagctc gatgggaccg cgcccgacgc aacgaagcgc ccgcacgtca
      961 tcaacaacag ttggggctgc cccgaaggct gcacgcccac caccctcaga gatatcatcg
     1021 aggcctcgca ggcttcgggt atcctggttg aggcttcggc gggcaacgac ggccctgcct
     1081 gctccaccgt caccgtgccc ccggctatcc acgaggcctc attcaccacc ggcgctctcg
     1141 acatccaccc cgaggacggc ctattcctgg ctgacttcag cagccgcggc ccggtggcaa
     1201 ccgatggctc gatgcgtctc aagccgaaca tctcggctcc cggtgtggat acacgctctg
     1261 ccgtcatagg cgagagcgat gctgaatacg acgagttcag cggcacgtca atggccgggc
     1321 cgcacgtggt cggagtggtc gctttgctct ggtccgcaag acctgagctg gcgcgccaga
     1381 ttaccgagac caaggtcctc ctccagatga gcgcaaatcc aggcgtagac gtgatagaac
     1441 cgataccgat gtgtggcggc acaacgcctg aggacatccc caacaacttc ttcggctggg
     1501 gcgctgttga cgccttagcc gccgtacaac gagcgcaggg catcgagcca accgccaccg
     1561 ccatcctgcc aacggcgaca ccgggcaccc ccacagccac tcccaccgca tgccccatcc
     1621 agttcactga cgtaccggcc gaccacacct tctacgcctc ggtgcgctgc ctgtcgtgca
     1681 ggcacatcat cggcggctat ccgtgcggtg gcatcaaccc cgacaccggc caggctgagc
     1741 cttgcaccgg caaccaacct tactaccggc ctagctcgaa cgtcacacgc gggcagatcg
     1801 ccaagatcgt tgcccagtcg ttcgagtata atgccgaccc aggcgagcaa atctacgcgg
     1861 acgtgaagcc gggcgacccg ttcttcacct ggatcaacag gctgaccgcc caggacgtga
     1921 tgaacggtta tccttgtggt gggcctggcg aaccgtgttc aaacggcagc aggccgtact
     1981 tccgccccgc gtcgtatgcc accagaggcc agctatcgaa gatcgtagcc aatgcggctg
     2041 cgttctacga gttccacaac aaccaggtgt tcgaggacgt gcctgcgacc cagccgttct
     2101 acatgtacat cgcacgcctg gcctcgcgcc agatcatgac cggctacccc tgtgggggca
     2161 ccaaccccga caccggacaa gcagagccgt gcggccaggg caacaagccc tacttccgcc
     2221 ccaactctaa cgtctccagg gggcaaacag ctaagattgt agccaatgcc gccttcccat
     2281 tctgtaacac cccgtaacac gaggtaggca ctgggaagag gaataagagg aagaaggggc
     2341 actgtaaggt gccccttctt cctgtctgcc tagccgttcg ccacccattc acacactctt
     2401 aagacatctt ttctacatca gaaacaaggg tacgcttcct gcacctactc tcccgcgcaa
     2461 tcctgtagac aatgccgtca acaaaaggag gagcagatgt ccactagaga gcctacacat
     2521 gcactgaggc ccaacatccg ggtgcttatg gccctggccc tattggtgct ggcgatgatg
     2581 gcgcttgggt ccggcgggtc gagcagtgcc gccagggcag cggcacctac cgtccccggc
     2641 gctaactccg cccagcccgc aggcaagatc gaccccttcg tgtcgcagga cctcgccggc
     2701 gacgggtccg cctccctcgt catccgcatg gctgacaagg ccgacctgcg cgccgcctac
     2761 ggtatgaaag accacgacgc gcgcggctgg tacgtctaca acacactgag ggaccacgcc
     2821 gccaggacgc agaccggcgt gcgcgctttc ctcgacgcac gaggcgtgag ctacgaatcg
     2881 tactgggggg cgaacgttat catcgcggag ggcaatcgcg agctggtgga ggtcctggcc
     2941 acgcgcaccg acgtggagag gatcgagtcg aatcgcccct tcaggggtat cgaccccata
     3001 cagagcgacc cctcggacaa gagcggcgcc aacagccctg atggggcaat cggcatcgcc
     3061 tggggcgtcc agaacgtgcg cgctcccgaa gtctgggcta tgggctaccg gggcgcgggc
     3121 atcgtcatcg gcaaccagga cacaggcatg cgctggacgc acgtcgcaat caaggaccag
     3181 taccgcggct ggaacggggc cactgcgaac cacaactaca actggtggga cggcctgcgc
     3241 gtgcccggcg acctccctac gaacccctgc ggctactcta caaatgagcc ttgcgacgac
     3301 gacgagctgc tcggcggcgg gcatggcacc cacacgactg ggaccgtcct gggcgacgac
     3361 ggcatgggca accaggtagg cgtcgctccc gaggcgacct ggatgggctg ccgcaacatg
     3421 gaacggggcg tgggccgccc gcagacctac atggagtgct tcgagttctt cctggccccg
     3481 cacgaccttg agggcaacaa ccccgacccc acaaagcgcc cccacgtcat gaacaacagt
     3541 tggatatgtg tggagggctg cgctcccaat accctgcaag accttgtaga ggcgtcgcag
     3601 gcttcgtgta tattcgtgga agtctcggcg ggcaacgacg ggcctacctg ctccaccatc
     3661 cagttccccc cgcagatcta cgaagcgtcc ttcgacacag ggtctatcac tgttgacgag
     3721 gaaagcggcc tacctatcct gtccgacttc agcagccgcg gtcccgtgct cagcgacggt
     3781 tcgatgcgca tcaagcccaa cgtcgtggca cccggcgagg atataccgtc tgccctacgg
     3841 ggtagcgaca ccgaatacgg gaacctggac ggcacctcta tggcagggcc gcatacggtc
     3901 ggcgtggtcg cgcttatctg gtctgcccgg cccgagttgg tgcgccagat caccgaaact
     3961 aaagtcctgc tccaactgag cgccaacccg gacgtacacg tggtcgatcc catacaagag
     4021 tgtggcggca ccacggaagc cgacatcccc aacaaccact tcggctacgg ccgcgttgac
     4081 gcgtttgccg ccttgcagag agcgcagggc atcgagccga ccgccactgc cattctggca
     4141 agcccgacgc cgggcacgcc caccgccacc cctaccgcct gccccatcca gttcgctgac
     4201 gtgcctgcca atcacacctt ctacgcctcg gtgcgctgcc tgtc
//
LOCUS       DASRWZ010000035         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_70955,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000035 DASRWZ010000000
VERSION     DASRWZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70955"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1333
                     /locus_tag="VFH60_00460"
     CDS             206..1333
                     /locus_tag="VFH60_00460"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6312278.1"
                     /translation="MKQQSHSGAGMRNAAAPSRSRLMALTILFSLMLAFTAFAGAVAA
                     SQPARQLAQAEQTPSAALPITDDSVPPATGNQPVGTAVAPDVVQTGLPVPVQTGVAPE
                     AIETTQAAVREAQGQDAPPPVQGDNPAAPGQAASQQSGFPWPWVVLAAMLALVIAGVM
                     LMRTRRTTPQTATVVQSGSVQPYGAPASSARVGSSGAPTTPIPVAPFAAPVGAEATVE
                     TPAPPSTVACPNCGTSNDFSENFCHECGQDLRPTRAQMVAALAPPPAQEDVVTEDTPY
                     LETLDRIDEQLEYVLARPKIAVGTAPGNDIVVDSSFKGWEQVSPVHAELRREQEGFVI
                     VDKDSASGTFVNEMRTGENILADGDMIRLGGVRFIFHIPSA"
     gene            1344..>3055
                     /locus_tag="VFH60_00465"
     CDS             1344..>3055
                     /locus_tag="VFH60_00465"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET6312279.1"
                     /translation="MNKMRRVGKNTNRPVVEQPVAEDAAVGVKDDSVDAAAPSPEVEA
                     QDQAPEAEAAVVPGDPVAVGQEAPVEGDAVVEAEEGEQPADVIPEEDTGGPVDYIEET
                     QDVEGDSLLYRESGVAAEALQDGQADEETVEVVRESGEAQPQTAPLQHVPEREDLTPL
                     PVNAVVDSRYVVQSQLHHSPERNLYRVTTRRQQRCASCGRPSPSDLDTCPQCGAALDG
                     QTPADFYLMAESYRPDALIQDAGMMDLHLYHPNLVPVIDFFNYKPFGKTRYFAVAEPR
                     QGVRLSQMSLPRPGAQVLGWAMQLADALDYLHNRGVVGAGAEADDILVQGDRASLASL
                     QNARTTITDGAEKLQQQTMDLARLAATIYEAYTGNAATISPEGMLPMPPGAPEQVGAA
                     FRAAIEPVQGVAPPISVEHWRNMLAQALEAVAELERPGRPVNIVSAGLTDVGRLREQN
                     QDSFGMAEFVQASAERPMKLGLYIVADGLGGHKGGEIASALAVQSFSGELIGRIRAPL
                     MAMTSDRATPSNEAILQGMTRAVQTANERIYKARGNRQNDMGTTLVAAVIAGGKAYIA
                     NVGD"
BASE COUNT          602 a          942 c         1005 g          506 t
ORIGIN      
        1 ctcatgtcga acggaaattt tgaattatga atgttgaatt ttgaattgtt tctcggttcg
       61 tgtttgtgcc tataattcaa aattcaatat tcataattca aaattcccgt tcgacatggt
      121 gctgccaact tgctataata acctccgggt tatcacactc gtgtggagcc acacaataca
      181 gataaacagg aggtagcttc tctctttgaa gcagcaatca cattccggtg caggcatgcg
      241 caacgctgcc gcaccttccc gctctcgcct catggcgctg actatactct tctccctcat
      301 gctggctttt acggctttcg cgggtgcggt ggcggcctcg cagcctgccc gccaactcgc
      361 gcaggcagag cagacgccca gcgccgcgct gccgataacc gatgatagcg tcccgcctgc
      421 tacgggcaac cagcctgtag gcaccgccgt cgcgccagat gtcgtacaga cgggcctgcc
      481 ggtgccggtg cagacgggtg tcgcgccgga agccatagag accacgcagg cggcggtacg
      541 tgaggcgcag gggcaagatg ctcctccccc cgtgcagggc gacaaccccg ccgcgcctgg
      601 gcaggcggca tctcagcaga gcggatttcc ctggccgtgg gtagtgctgg ccgccatgct
      661 cgcgctggtg atagcgggcg tcatgcttat gcgcacgcgc cgcaccacac cgcagacggc
      721 tacggtcgta cagagcggct cggtgcagcc ttatggcgcg cctgccagca gcgccagggt
      781 tgggtctagc ggagcgccca ccactccgat tcccgtcgct ccgttcgccg cgcctgtggg
      841 cgcagaggct accgtagaga cacccgcgcc acccagcaca gtcgcgtgcc cgaattgcgg
      901 cacgtccaac gacttcagcg aaaacttctg ccacgagtgc gggcaggacc tgcggcccac
      961 gcgggcgcag atggtagccg ccctcgcacc ccctccggcg caggaggacg tggtcaccga
     1021 ggacacaccc taccttgaaa cgctcgacag gattgacgag cagctagagt acgtgctggc
     1081 gaggccgaag atagccgtcg gcaccgcgcc cggcaacgac atagtggttg attcgtcgtt
     1141 caagggctgg gagcaggtgt cgcccgtgca tgcggagctt cgccgcgagc aggaaggctt
     1201 cgtcatagta gacaaagact ccgcgagtgg cacgttcgtc aacgagatgc ggacgggcga
     1261 gaacatcctc gcggacgggg acatgatacg cctgggtggg gtgcggttta tcttccatat
     1321 accttcagcg taagagagag taaatgaaca agatgcgtcg cgtgggcaag aacaccaacc
     1381 ggcctgtagt cgaacagcct gtagccgaag atgctgctgt tggggtcaaa gacgacagcg
     1441 ttgatgcagc agccccctcc cctgaggtag aggcccaaga ccaggcaccg gaggcagagg
     1501 ccgcagttgt gcctggggac ccggtcgcgg tcggccagga ggctccggtt gagggcgacg
     1561 cggtggtcga agctgaagaa ggtgagcagc ctgcggatgt cataccagag gaagataccg
     1621 gcgggcctgt tgattatata gaggagacac aagacgtaga gggcgacagc ctcctctaca
     1681 gagagagtgg ggtcgcggcg gaagccttac aggacggcca ggcggacgag gagactgtgg
     1741 aggtagtccg cgagagcggc gaggcgcaac cgcagacggc gcctctccag cacgtgcctg
     1801 agcgggaaga cctcacgccc ctgcccgtga acgcggtagt tgacagccgc tacgtcgtgc
     1861 aaagccagtt gcaccacagc cccgaacgca acctctacag ggtgacgacg cgccgccagc
     1921 agcgctgtgc cagttgtggc cgtccttctc cctccgacct cgacacctgc ccgcaatgcg
     1981 gggccgcgct cgacgggcag acccccgccg acttctacct gatggctgag agctaccggc
     2041 ccgacgcgct catacaggac gcgggtatga tggacctgca cctgtaccac ccgaacctcg
     2101 tgccggtgat tgatttcttc aactacaagc ccttcggcaa gacgcgctat ttcgccgtcg
     2161 ctgagccgcg ccagggcgtg cgcttgagcc agatgagcct tccccggcca ggagcgcagg
     2221 tgcttgggtg ggccatgcag cttgccgacg cgctcgacta cctccacaat cgtggcgtgg
     2281 taggtgcggg cgcggaagca gacgacatac tggtgcaggg cgacagggcg tcgctcgcca
     2341 gcctgcaaaa cgcccgcacc accatcaccg acggggcgga gaagttgcag cagcagacta
     2401 tggacctggc gaggctggcc gccaccattt acgaggcgta caccggcaac gccgccacga
     2461 tcagtcccga aggtatgctg ccgatgccgc cgggcgcgcc tgagcaggtg ggggcggctt
     2521 tccgcgccgc catcgagcct gtgcaggggg tcgcgccgcc catcagcgtg gagcactggc
     2581 gcaatatgct ggcgcaggcg ttggaggcgg ttgccgagtt ggaacggcct gggcgtccgg
     2641 tcaatatcgt gtcggcgggc cttaccgatg tgggtaggct gcgcgagcag aaccaggact
     2701 cattcggcat ggccgagttc gtgcaggcga gcgcggagcg gcccatgaag cttgggctgt
     2761 acatcgtggc cgacgggctg ggcgggcata agggcggcga gatagccagc gccctggcgg
     2821 tgcaatcgtt ctcaggagag ttgatagggc gtatcagagc gccgctcatg gcgatgacct
     2881 ccgaccgggc aacacccagc aacgaggcta ttttgcaggg gatgacgcgg gcggtgcaga
     2941 ccgctaacga acgcatatac aaggcgcgcg gcaaccgcca gaacgacatg ggcaccaccc
     3001 tggtcgccgc cgtgatcgcc gggggcaagg cgtatatagc taacgtgggc gatag
//
LOCUS       DASRWZ010000036         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1098827, whole genome shotgun sequence.
ACCESSION   DASRWZ010000036 DASRWZ010000000
VERSION     DASRWZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098827"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VFH60_00470"
     CDS             <1..1109
                     /locus_tag="VFH60_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6312280.1"
                     /translation="DAGTGYGISGKLVVAGGHSNPPTASNYLVPSNLAYIFVPPSAPD
                     LDGTWSPFIGLTNPTRNFGFAAHDGFLYAIGGYDYSAGKPDSAGYTQRFDANGDIATT
                     TPVPTSTNTATPTSTVTGTPPTAIPTSTATPIPSATSVACDIEFADVAAGSTFYSFVR
                     CLACKDVLSGYPCGGPGEPCNSRGDPFFRPGNNITRGQIAKIVSESARIYEAPQQQIY
                     EDVAPDSPFYQWINRLSRRGYMSGYACGTTPAEACGPDNLPYFRPNALATRGQLSKIV
                     ANAAELDEAATGQTYADVPESSDPSSFYPYIERLTERNVMGGYACGTTDPNSGPCDGQ
                     ARPYFRPSNHVTRGQAAKIVANTFYPACDTPARP"
     gene            1252..2229
                     /gene="uvsE"
                     /locus_tag="VFH60_00475"
     CDS             1252..2229
                     /gene="uvsE"
                     /locus_tag="VFH60_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HET6312281.1"
                     /translation="MRLGFACTVMGLDVRAHDTRKWQNNPHLRVSLENVAKILHYAHG
                     LGISMYRLSSNLAPYYTDPSRPQFSGQLEESRQALADTGALARGLDIRLSMHPGQYTV
                     LNSPNEAIYQAAVRELRYAADVLDYMQMPYDNKVIVHIGGAYGDKGPALQRWIERYPL
                     LPANVRARLVIENDDTIFNVADALYVSGHTGVPIVFDNLHHRVNPSALSGHDVALDEA
                     AALHLCLDTWPADQRPKVHYSDQRTQPVLVRVKGKGTRTQAPSAGAHADYVDPAGFLA
                     FLQRAGDPRFDVMFEAKAKELAVLRVMDYVRGKDTEPPRHQGRQGPRQD"
     gene            2498..>3741
                     /locus_tag="VFH60_00480"
     CDS             2498..>3741
                     /locus_tag="VFH60_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6312282.1"
                     /translation="MTGELNIVSTLSRPTVPVMNVPQLVYCLLEMRPAEAVSAAPMPL
                     NLCLVLDHSGSMNGSNKIENLRDATNLVLDMLTPQDYIAIIAFNSRNEVIFSGQIRDS
                     SVRSDLRGRIARLKADGGTKMAPAMEAGLVELRKQMPAPGSNAPTALVNRLVLLTDGI
                     TEKEKHCLEQADNVARLGVPVTALGIGKDWNDKLMEEIGRRTGGDADYISSPERIRSH
                     FQRAVQQMQAVALQRLSLGVKPALGIEVRSLYRVHPLISRLAPGQEAGDLLVPLGELE
                     RGHGQTLLVEFVVPSRPAGTYRIAQLEAAYDVPQSGLSAQRANLDVVLSYSHDPAVIS
                     PPNPRVMNLVEKVSAFKLQTRALQDLEAGNIPAATQKLKGALTHLLNQGDTELAGVVQ
                     QELANLEKGRVMSPEGRTTIRF"
BASE COUNT          738 a         1298 c         1051 g          654 t
ORIGIN      
        1 atgacgcggg tacgggctac ggcatctcag gcaagctcgt ggtggcgggc ggccattcca
       61 acccccctac cgcaagcaac tacctggtgc ccagcaacct ggcgtatatc ttcgttccgc
      121 cctcagcgcc cgacctcgac ggcacgtgga gccccttcat cggcctcacc aacccgaccc
      181 gcaacttcgg gttcgcggcc cacgatggct tcctctacgc aatcggcggg tacgactaca
      241 gcgccggtaa gcccgactcc gccggttaca cccagcgatt cgacgctaac ggtgacatcg
      301 ccaccacgac ccctgtgcct acttccacca acactgccac gcccaccagc accgtgaccg
      361 gcaccccgcc gaccgccata cccaccagta cggcgactcc tatcccctcc gctacgtccg
      421 tcgcctgcga tatcgagttt gccgacgtgg cggccggtag caccttctac tcgttcgtgc
      481 gctgcctggc gtgcaaggac gtgctgagcg gctacccgtg cggcgggccg ggcgagccgt
      541 gcaactcccg cggtgaccca ttcttcaggc cgggcaacaa cattacgcgc ggccagatag
      601 ccaagatcgt ctctgagagc gcccgcatct atgaggcccc tcaacagcaa atctacgagg
      661 acgtggctcc cgactcgccc ttctaccagt ggatcaaccg cctgtcgcgg cggggctaca
      721 tgagcggcta cgcgtgcggc acgacccccg ccgaggcttg cggccccgat aacctgccct
      781 acttccgccc caacgccctg gcaaccaggg gccaattgtc gaagatcgta gccaatgccg
      841 ccgagctgga cgaggcagcg acgggacaga cctacgccga cgtgccggag agcagcgacc
      901 ccagttcctt ctacccctac atcgagcggc taaccgagcg taacgtcatg ggtggctacg
      961 catgcggcac caccgacccc aacagcggcc cctgcgacgg ccaggcccgc ccttacttcc
     1021 gcccctccaa ccacgtcacc aggggccagg ctgccaagat cgtagccaat accttctatc
     1081 ccgcctgcga cacacccgcc cgcccataat acgagaacca gacctcaatt gcctgcgtag
     1141 aaagagagga gagactgttc ccctctcttt ctttttggcc tggtgcacga tttgtaatgc
     1201 gggagaagca actgccacca tgcaagatag aattggcagc aggagagaat aatgaggctg
     1261 ggtttcgcat gtacggttat ggggctggat gtgagagcgc acgatacgcg caagtggcag
     1321 aataacccgc atctgcgcgt gagcctggag aatgtcgcca aaatacttca ttacgcgcac
     1381 gggttgggca taagtatgta ccgcctaagc tccaacctcg cgccttacta caccgacccc
     1441 agccgcccgc agtttagcgg acagcttgag gagtcgcgcc aggctcttgc ggataccggt
     1501 gcgttggccc gcggccttga catccgcctc agcatgcacc ccggccaata tacggtgctc
     1561 aattccccga atgaggctat ctaccaggcc gccgtccgcg agcttcggta cgccgccgac
     1621 gtgctcgact acatgcagat gccgtacgat aacaaggtga tagtgcatat aggtggggca
     1681 tacggcgaca aggggcccgc actacagcgc tggatagagc gttacccgtt gctccccgca
     1741 aacgtgcgcg cccgcctcgt gatagagaac gacgacacta tcttcaacgt ggccgacgcc
     1801 ctctatgtga gcggccacac aggggtgccc atcgtgttcg acaacctgca ccacagggta
     1861 aaccccagcg ccctctccgg ccatgacgtg gcgcttgacg aggccgccgc cctccacctc
     1921 tgcctggaca cctggcccgc ggaccaacgc ccgaaggtcc actattcgga ccagcgcacc
     1981 cagcccgtac tggtgcgtgt gaaaggcaaa ggcacccgca cccaggcacc ctcagccggt
     2041 gcacacgctg actacgtaga ccccgccggg ttcctggcct tcctccagcg ggcaggggac
     2101 ccacgctttg acgtaatgtt cgaagccaaa gccaaggaac tcgccgtcct ccgcgtcatg
     2161 gattacgtgc ggggaaaaga tactgaaccg ccaagacacc agggacgcca gggaccacgc
     2221 caagattaag ataaagacgt gttctaaaca aagaggagca aggtacgtga tgtcgtacct
     2281 tgctcctctt tgtactcact aaaccgtcct aaatctttgc gcgttctttg cgtctttgcg
     2341 tctttgtgtt ttcgtactct cggcgtggtc ccggcgtcct tggcgtaatg cggttccgta
     2401 tcttttcctc tcccgcgcaa ctttgacgcg tgccgaacgt aggtctataa tggttgggcg
     2461 gcagtcttgt actcagcatt ctagttcgga gagcgttatg acaggtgagt tgaacatcgt
     2521 ttctaccctt tcgcggccca cggtgcccgt gatgaacgtg ccccagcttg tctattgttt
     2581 gcttgagatg cggcccgccg aggccgtttc cgccgctccg atgcctctca acctgtgcct
     2641 ggtgctcgac cacagcggct cgatgaacgg ctccaacaag atcgagaacc tgcgcgatgc
     2701 taccaacctg gtgctcgata tgctcacgcc ccaggactac atcgccatca tcgctttcaa
     2761 cagccgcaac gaggtgattt tctcgggcca gatacgcgat agctcggtcc gctccgacct
     2821 gcgcggccgt atcgctcgcc tcaaagccga cggcggcacc aaaatggccc ccgcgatgga
     2881 ggccgggctg gtagagcttc gcaaacagat gcccgcgccc ggctccaatg ctcccaccgc
     2941 cctggtgaac aggctcgtgc tcctcaccga cggcatcacc gagaaggaga agcattgcct
     3001 cgaacaggcc gacaacgttg cccggctcgg cgtgcccgtt accgcgcttg gtatcggcaa
     3061 ggactggaac gacaagctga tggaggagat aggccgccgc accgggggtg acgccgacta
     3121 catctcgtcg ccggagcgca tccgctccca cttccagcgg gcggtacagc aaatgcaggc
     3181 cgtggccctt cagaggctct ccctcggcgt caaacccgcg ctcggtatag aagtgcgcag
     3241 cctctacagg gtgcaccccc tcatctcgcg tctcgcgccg ggacaggagg cgggcgacct
     3301 gctcgtgccg ctgggcgagc ttgagcgggg gcacggccag accctgctgg tggagttcgt
     3361 ggtgccctcg cgcccggcgg gcacctaccg cattgcccag cttgaggccg cgtacgacgt
     3421 gccacagagc gggctatccg cccagcgcgc caacctggac gtggtgctca gctactcgca
     3481 cgaccccgcc gtgatttccc cgcccaaccc gcgcgtgatg aacctcgttg agaaggtgag
     3541 cgccttcaag ttgcagaccc gcgccctgca agacctggag gcaggcaaca tccctgccgc
     3601 cacccagaag ctcaagggcg cgctcaccca cctgctcaac cagggcgaca ccgagctggc
     3661 cggagtagtg caacaggaac tggccaacct cgaaaaaggc cgcgtaatgt cccccgaagg
     3721 ccgcacgacg atccgcttcg a
//
LOCUS       DASRWZ010000037         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_139616, whole genome shotgun sequence.
ACCESSION   DASRWZ010000037 DASRWZ010000000
VERSION     DASRWZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139616"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2295)
                     /locus_tag="VFH60_00485"
     CDS             complement(<1..2295)
                     /locus_tag="VFH60_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6312283.1"
                     /translation="MLVVAGAGAVSPLFVPRDSAFGVVLTIASLCVALALGLVLLIGA
                     VVARRWTSALVTVLDDLSQGRYSPRNVPGSEDGEIGSLSRAVDTVASQLQDRFSRLEQ
                     TEVRFRSLLSCLPGVPYIYNACQDGDIEVLFVSPKVQELLGFTQAEWLSDPTLWSCQL
                     HNDDRARVLAQYTAACERGETFASEYRMLTRDGRTVWIRDEAQITQSSDGNGQLMLGM
                     LLDVTWQRNEKQLLHERADRFVGVLDTAREAIISTDHQGNVTLFNQGAQTMLGYTEAE
                     VLGKNLAALWPPDKARGYARQLTDFAGTDNRTGKLGNLGEVLVCRKDGTQILAEASIS
                     QYSRGADKTITVVMRNITERKRIEEHIKSGEASFRSMFSENPLPMWVYDLESLYILEV
                     NSAAQARYGYTRQEFIAMRMTDLKAHEEMPRLVENGNGNKVNDGQYSIQIKHRCKDGR
                     VMDVQLVSQGMEFAGHRAAMVVAEEITERKKAEEALRESEQRFRAMFEGAAIGTALVD
                     LDGHYIKANPALERMLGYASDELRNIRLGDITHTDDLQRSMEPFTQLVNGHKDFYHIE
                     QRFVCRDGSVTWANLTMSLVRGNRRKPQFCIAMVENITERKNTQEQVKRQLERLAALR
                     NIDMTISSSLDLRVTLNVILEQVMSQLHVDATDVLLHNPHTQMLEYAAGRGFKYEGIT
                     RTRLRLGEGNAGVAALERRTVNVPNLHLEADPSREILLAGEEFVTYYAVPLLVKGQVK
                     GVLEIFNRTHLNPQLEWRDFLEALT"
BASE COUNT          412 a          771 c          806 g          479 t
ORIGIN      
        1 ggtaagggcc tctaggaagt cgcgccactc cagctgcggg ttcaggtggg tgcggttgaa
       61 gatctccagc acgcccttca cttgcccctt gacgagcagc ggcacggcat agtaagttac
      121 gaactcctcg cccgccagca atatctcgcg gcttgggtcc gcctcaaggt gcaggttggg
      181 cacgttcacg gtgcggcgtt cgagagcggc cacgcctgcg ttgccctcac ccagccggag
      241 ccgcgtgcgc gtgatgccct cgtacttgaa gccccggccc gccgcgtatt ccagcatctg
      301 cgtgtgcggg ttgtgcagca gcacgtcggt ggcgtccacg tgaagctggc tcatcacctg
      361 ttcgaggatt acgttcagag tcacgcgcag gtcgaggctc gagctgatcg tcatgtcgat
      421 gttgcgcagg gctgccaggc gctctagctg cctcttgacc tgctcctgcg tgttcttgcg
      481 ctcggtgatg ttctccacca tcgcgatgca gaactggggc ttgcggcggt tgccacgcac
      541 cagcgacatg gtaaggttgg cccaggtcac gctgccgtcg cggcacacga accgctgctc
      601 gatatggtag aagtccttgt gcccgttcac aagctgggtg aaaggctcca tgctgcgctg
      661 caaatcgtcg gtgtgagtaa tgtcgcccag gcggatgttg cgcagttcgt cgctggcgta
      721 gccgagcatg cgctccaacg cggggttggc cttgatgtag tgcccatcga ggtccaccag
      781 cgcggtgccg atagcagcgc cctcgaacat ggcgcggaac cgctgctcgc tctcgcgcaa
      841 cgcctcttcc gccttcttgc gctcggtgat ctcctcggcc accaccatag cggcgcggtg
      901 cccggcgaac tccatgccct gcgacacgag ctgcacgtcc atgacccgcc cgtctttgca
      961 gcggtgcttg atctggatgg agtactggcc gtcgttgacc ttgttgccgt tgccgttctc
     1021 gaccaggcgc ggcatctctt cgtgcgcctt caggtcggtc atgcgcatgg cgatgaactc
     1081 ctggcgggtg tagccatagc gggcctgcgc cgcgctgttc acttccagga tgtagaggga
     1141 ctccaggtcg tacacccaca tcggcagcgg gttctcgctg aacatggagc ggaagctcgc
     1201 ctcgcccgac ttgatgtgct cctcgatgcg tttgcgctcg gtgatgttgc gcatcacaac
     1261 cgtgatagtc ttgtcggccc cgcgcgagta ctgggagatg gaggcctcgg ccagaatctg
     1321 ggtgccgtcc ttgcggcata ccagcacctc gcccaggttg cccagcttgc ccgtgcggtt
     1381 gtcggtgccc gcgaagtcgg taagctggcg agcatagcca cgcgccttgt cggggggcca
     1441 tagcgccgcc aggttcttac ccaggacctc ggcttcggta taccccagca tcgtttgcgc
     1501 gccctggttg aagagggtca cgttgccctg gtggtcggtg gagattatcg cctcgcgtgc
     1561 cgtatcgagc acgccgacga agcggtcagc ccgttcgtgc agcagttgct tttcgttgcg
     1621 ctgccaggta acgtcgagca gcatgccgag catgagctgg ccgttgccat cgctgctctg
     1681 cgtaatctgg gcctcgtcgc gaatccacac cgtgcggccg tcgcgcgtca gcatgcggta
     1741 ctcggaagcg aaagtttcgc cccgctcgca ggcggcggta tattgcgcca gcacgcgcgc
     1801 ccggtcgtcg ttgtgaagct ggcaggacca cagggtgggg tcgctcagcc actcggcctg
     1861 cgtgaagccg agtagctcct ggactttggg gctgacgaat agtacttcga tgtcgccgtc
     1921 ctggcaggcg ttgtagatat agggcacgcc gggaaggcag gagaggagag agcggaaccg
     1981 cacctcggtc tgctccagcc tgctaaagcg gtcttgaagc tgggaggcca cggtatcgac
     2041 tgccctcgac aggctgccaa tctcgccgtc ttcgctgccg ggcacgttgc gaggggagta
     2101 ccggccctgc gacaggtcgt ccagcacggt gacgagcgcg gaggtccagc gacgtgccac
     2161 caccgcgccg atgagcagga cgaggccgag ggccagggcc acgcacaggc tggcgatggt
     2221 gagcacgaca ccaaaggcgc tatctcttgg cacaaacagg ggggaaaccg caccggcccc
     2281 ggcgacaacc agcaagagga ctgcacagag caggcgagtg cgcacaaaga gggcaacaag
     2341 cgtagcgagc ccgttgccgg gttgtcgctc gcgtttggtt gtggttccgt gtgtcttctc
     2401 gccgcctgcg cgcacggaag ctatggaact aaacagcaat gaaccctcca agaaaagacg
     2461 aagcaagt
//
LOCUS       DASRWZ010000038         8908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1304570, whole genome shotgun sequence.
ACCESSION   DASRWZ010000038 DASRWZ010000000
VERSION     DASRWZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304570"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..346
                     /locus_tag="VFH60_00490"
     CDS             206..346
                     /locus_tag="VFH60_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312284.1"
                     /translation="MAEQLMGAIPAPPFPEGLDWIHTGGKALRLEDLRGKLVLLDFMT
                     YG"
     gene            356..1729
                     /locus_tag="VFH60_00495"
     CDS             356..1729
                     /locus_tag="VFH60_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase"
                     /protein_id="HET6312285.1"
                     /translation="MHILSAVRRLEEEYSDTLVAIGVHSGKFIAERGTANIRTATHRL
                     HIHHPVVNDRHFRTWRAYNVSAWPTVVLVSPDGKIIGMHAGEFTFEDFDKVIYALEQV
                     YDKAGMLDRTPLNFPLDPEPPADSPLRFPGKVLADHSSGRLFIADTGNNRVLVARVAG
                     ESEARVVQTIGTGEAGMRDGAFAEAALNSPEGMALSGDTLYIADTENHSIRAAHLDSG
                     TLETVAGTGEQGYSRTGGLGKSTALSSPWDLLVRDNWLYITMAGTHQLWRMDLQSGEV
                     LPFVGSGRENIDDGPNPRATLAQPSGLTTDGQRLYFADSESSAVRASDFDPNGHTQTL
                     LGEGLFEFGDKDGKGLSVRLQHTLGVSYHNGKIYIADTYNNKVKTYDLATKECHTSLG
                     SGNSEELYEPGGLSVWAAEGEPRLYVADTNNHRVLHSAIDEAGTLLPPVPLRISFASV
                     AGNSAGQ"
     gene            1912..2961
                     /locus_tag="VFH60_00500"
     CDS             1912..2961
                     /locus_tag="VFH60_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6312286.1"
                     /translation="MSRLRNCLPSHFIRRPPPLEDKATIEGNPDRELGLLSAVPPTVR
                     PARGVRVAGRRWADFGSALLYMSPALLVFAAFTFIPFFRSIWLSFFVTDQTGSPARFN
                     DIKYYARILNLDGSGRDEYLNSILTTVKFTLMVVPTGIAVAVGLAVLATAKVRGMSIF
                     RTIFTSTVAISVASASVIWALIYNPSLKMTTWVVDLLNLKTQGLLLDPATALPAVAFM
                     TIWTSLGFNFIITLAGLQAIPQDLYDSSRIDGASGWQLFRFITLPLLTPTLLFLLIIS
                     TIGAFQAFTQFNVLIANEGPDASTNVMVYALFSSFFKDNRYGFASALAVVLFVVLLVL
                     SIVQFKLLDRRVHYQ"
     gene            3009..3827
                     /locus_tag="VFH60_00505"
     CDS             3009..3827
                     /locus_tag="VFH60_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018886648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6312287.1"
                     /translation="MTGLLKSAGLYALLVLAALILLFPLIFSLSLALQGPTISPNLLP
                     DFANLDWGVFAATFAQEPNFGRWILNSFVVALAVTVGQLITCSLAAYALSTLSFPGKN
                     FFFFVFLATLMIPWEATIVPNYLTVVGLNWKDTYQGLIAPFLASGFGIFLLRQYFMTI
                     PRELFEAAVLDGCGRTRYLWSILLPLSRPALGTLAVYAFLNTYNQYYWPLLVTDSPEW
                     RTTQVGITAFKSSEVALFNLQMAGTLIVMLPTLILLVVGQRQLVRGLTAGALKG"
     gene            3942..5543
                     /locus_tag="VFH60_00510"
     CDS             3942..5543
                     /locus_tag="VFH60_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6312288.1"
                     /translation="MAMTRNFRQIISISFIVILLTSMLAACGGPTTTPTTVSNTATTA
                     PAVDTATTAPANDTPTTEAMADTPTTAAVAPTATTAGASTTMTATTAPAPTATPYPTV
                     PAAAGATKITFWFGLTGFNGGVTQEVVNRFNTSQTKYYVEAIQQPNYDDTINKLSTSF
                     AGGALPNVVQIYDIGTQRMIDSKRIVPVQDFLDKENSDIAADLEPAVASYYTVNGKLY
                     SMPFNSSAPVMYYDKNAFREVGLDPEKPPTTYDELFAAAQKLVKKDASGKIVRSGVDF
                     TLYGWILEQQLAIQTTVYAEPDNGRGAERATRVNFNSEAGQNWLNFLKKLQDEGVGRS
                     LGRHSGTTNGTTLGANFSRGDAAIAFESIATLRNWSRQAEAAGGKVDVGVAFLPKPTG
                     AKGGVIIGGASLWITDQGSAEQQQGAWEFVKFTAQPETQSFFASSTGYYPTRRAAYEL
                     PEMKEALVQFPQFQVAVDELRSTEPSPATQGAVFGTFAGTRVLVEGAMEQFMLGEAPT
                     AKAALDEAADEANELLEEYTSTLTP"
     gene            5635..7344
                     /locus_tag="VFH60_00515"
     CDS             5635..7344
                     /locus_tag="VFH60_00515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET6312289.1"
                     /translation="MQLLSHRERDAALHRMANEGVDILVVGGGITGAGVALDAAARGY
                     RVGLVEKSDFSSGTSSKSTKLVHGGIRYLAQFDFALVRESLVERGRLMRNAPFLVQPV
                     GFLLPLYAGAKRPMGMPIRPPFGLGMGLLLRAGLLAYDLMSGRLGIKRHRQISKATAA
                     RLYPCLNTSKLEAAFIYYDAQTDDSRLTATVIGTAARHGALVANHAELKGFQQENGKI
                     TAALVQDGISGRSFTIPTGTVVNAGGAFAGRIEELAGPSMIRIKPAKGVHLTVERQAL
                     KLGRGAIVLPETDDRRLLFLVPWGAHVTIGTTDTEGGDIDRPRADQDDVAYLLRHVNR
                     YMRCNLQESDIISAWAGYRSLVTSRKSSKASSRLSRTHAVLDGPGGMVTIVGGKLTTY
                     RRMAQDTIDHIARQQGKRVARATERLPLSGSEDWPRAHEDLNAAAARFQLKPDTARRL
                     AMYGSSSRVILDLITAAPDLAARIVPGLPYIMAEVVYACRYEMALDLADLLERRLRIS
                     FEDRSHGVKVAPSIAALMAEELSWDETEVHRQVNNYRNLIAESFQYGTTAGEAERVGA
                     HGR"
     gene            7334..8848
                     /gene="glpK"
                     /locus_tag="VFH60_00520"
     CDS             7334..8848
                     /gene="glpK"
                     /locus_tag="VFH60_00520"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET6312290.1"
                     /translation="MAANASPASRYILALDQGTTSSRAIIFDRAGHVVGQCSREFRQL
                     YPQPGWVEHDPEEIWASQLEVARKVLRDSDIAPGRIAAIGVTNQRETTLAWDSDTGEP
                     ICNAIVWQDRRTAQLCDSLKESGWGSKIQAKTGLVIDPYFSGTKLKWILDNVPGAAQS
                     AASGKLRFGTVDTFLVWRMSGGSLYITDYSNASRTMLFNIHELRWDEEILAGLDIPAS
                     ILPTVYPSSHLYGYAAESFLGEKIPITGVAGDQQAATFGQACYSPGMAKNTYGTGCFL
                     LMNTGRVAVPSKHNLLTTVGWGLGGEITYCLEGSVFSAGAVVQYLRDSLRLIDDAAQT
                     ETLALSVESSGGVYFVPAFVGMGAPYWDPYARGAVLGLTRGSGRAEIVRAALESVAYQ
                     TRDVLEAMVADSGEALTELRVDGGMVVNNFLMQFQADILGVPVVRSAIGETTALGAAY
                     LAGLAVDYWQSEEEIARNWSAAQVYEPRIDPASREALYRNWGRAVERSLRWEQP"
BASE COUNT         1764 a         2875 c         2653 g         1616 t
ORIGIN      
        1 agaatgacaa agggggatac aggctgacaa ggttgggctt tgttcgtcgc cgttgggcca
       61 taccctggcg cggccttata ccctcgcgca ataggcatta cgtccaagcg gcatctgctc
      121 tataatgctg gtggtggcgt aacgagcgca ctctgtaagt tccataccgg agttccctat
      181 cccgcaggcg caaggagcac gcacaatggc agaacaactc atgggcgcta tccccgcgcc
      241 tcccttccct gaggggctgg actggataca caccggcggc aaggcgctgc ggttggagga
      301 cctgcgcggc aagctggtgc tgctcgactt catgacctac ggctgaatca actgcgtgca
      361 catactgtct gccgtgcgca ggctagagga agagtactcg gatacgctgg tggctatagg
      421 cgtgcattcg ggcaagttca tcgccgagcg cggcaccgcc aacatccgca ccgccaccca
      481 caggctgcac atccatcacc ccgtcgtcaa cgaccggcat ttccgcacct ggcgggccta
      541 caacgtgtcc gcctggccca cggtggtgct ggtcagcccc gacggtaaga ttatcggcat
      601 gcacgcgggc gagttcacct tcgaggactt cgacaaggtc atttacgccc ttgagcaggt
      661 gtacgacaag gcgggcatgc tagaccgcac cccgctcaac ttccccctcg accctgagcc
      721 tcccgccgac tccccgctcc gcttccccgg caaggtgctg gccgaccact caagtggccg
      781 cctgttcatc gcggataccg gcaacaatcg cgtgctggta gcgcgggtgg cgggcgaaag
      841 cgaggcgcgg gtggtgcaga ccatcggcac cggcgaggcg ggcatgcggg acggggcctt
      901 tgccgaagct gccctcaact cgcccgaagg tatggcccta agcggcgaca ccctctacat
      961 cgccgacact gaaaaccact ccatacgcgc cgcccacctc gactccggca cgcttgagac
     1021 ggttgcgggc accggcgaac agggctattc tcgcacaggc gggctgggca agtccaccgc
     1081 gcttagctcg ccgtgggacc tgctggtcag ggacaattgg ctctatatca cgatggcggg
     1141 cacgcaccaa ctctggcgta tggacctgca aagcggcgag gtgctcccgt tcgtgggcag
     1201 cgggcgcgaa aacattgacg acgggccgaa cccccgcgcc accctcgccc agccgagcgg
     1261 ccttactacc gacggccagc gcctctactt cgcggacagc gagtccagcg ccgtccgcgc
     1321 ctccgacttc gaccccaacg gccacactca gacgctgctc ggagagggct tgttcgagtt
     1381 cggcgacaag gatggcaagg gcctcagcgt gcgcctgcaa cacaccctcg gcgtgagcta
     1441 ccacaacggc aagatataca tcgccgacac ctacaacaac aaggtcaaga cctacgacct
     1501 ggcgaccaag gagtgccaca ccagcctcgg ttcgggcaac agcgaggagc tatacgagcc
     1561 gggcggcctc agcgtctggg ccgcggaggg cgagccccgg ctgtacgtgg ccgacaccaa
     1621 caatcaccgg gtgctgcact ctgccataga cgaagcaggt acgctgttgc cccctgtacc
     1681 gttgaggatt agcttcgcat ctgtggcagg caacagtgcc ggacaatagc atgtcctccc
     1741 agtgagaatg actgcaccct ggccctgcaa taccgtattg aatgagcggc tgtagtgtgc
     1801 tatactcgcc gtgcctgctc ccgtgtgtag tagacaaaat actcacccag tagctggtgc
     1861 cgtagctgcc caacatcccc ggtgcctttt ctacatcgaa cggaagttag cttgagcagg
     1921 ctacgtaatt gcctaccctc gcacttcatc cggagaccgc cacccctgga agataaagcc
     1981 acaatcgagg gcaacccgga tcgcgagctt ggcctgttga gcgccgtgcc tcctacggtg
     2041 cggcccgcca ggggtgtgcg cgtggccggc aggcgctggg ccgacttcgg ctcggccttg
     2101 ctctatatgt ctcccgcgct gctcgtcttt gcggccttta cctttatccc cttcttccgc
     2161 tccatctggt tgagcttttt cgtcaccgac cagaccggtt cgcccgcgcg cttcaacgac
     2221 atcaagtatt acgcgcgcat tctgaacctc gacggcagtg ggcgtgacga gtacctcaac
     2281 agcatcctga caaccgttaa attcaccctg atggtggtgc cgaccggtat cgcagtagcc
     2341 gttggcctgg ccgtgctggc tacagccaaa gtgcgcggca tgagcatctt caggaccatc
     2401 ttcaccagca ccgtcgccat ctcggttgcc agcgcgagcg tcatttgggc gctcatctac
     2461 aatccgagcc tcaagatgac tacctgggtg gtggacctgc tcaacctgaa gacgcaaggt
     2521 ctgctccttg accccgcgac cgcgctgccc gccgtcgctt ttatgacgat ctggaccagc
     2581 ctcggcttca acttcatcat tacgctggcc gggctgcagg ccataccgca ggacctgtac
     2641 gacagcagcc gcatagatgg cgcgagcggc tggcaactgt tccgctttat tactctcccc
     2701 ctcctgacgc ctaccctgct tttcctgctg attatctcga ccataggagc tttccaggcc
     2761 tttacgcagt tcaacgtgct catcgccaac gaagggcctg atgcctccac taacgtgatg
     2821 gtctatgccc tgttcagctc cttcttcaaa gacaaccgct atggcttcgc ttcggccctg
     2881 gcggtcgtgc tgttcgtggt gctgttggtg ctcagtatcg ttcagttcaa gctgctcgac
     2941 aggagggtac actaccagtg agcacggtta cccaggccat taggagaggc cgcaacgact
     3001 ccctttctat cacggggctg ctcaagagcg cgggcttgta cgccttgctg gttctggccg
     3061 cccttatctt gctgttcccg ctgattttct cgctctccct ggcgctccag gggccgacca
     3121 tatcccccaa cctcctgccc gacttcgcta acctcgactg gggggtcttc gctgctactt
     3181 tcgcgcaaga accgaacttt ggccgttgga tactcaactc tttcgtggtg gccctcgccg
     3241 tgacggtagg gcagcttatc acctgctccc tcgcggccta cgccctgtcc acgctgtcgt
     3301 tccccggcaa gaacttcttt ttcttcgtct tcctcgccac gctgatgatc ccctgggagg
     3361 ccacaatcgt tccaaactac ctgacggtgg tgggcctcaa ctggaaagac acctaccagg
     3421 gcttgatcgc gcctttcctg gcgagcggct tcggtatctt cttgctgcgc cagtacttca
     3481 tgaccatccc ccgcgagcta ttcgaggccg ccgtgctgga tggctgcggg cgcacacgct
     3541 acctctggtc catactgcta cctctgtccc gccccgcgct gggcacgctg gccgtttacg
     3601 cgttcttgaa tacctacaac cagtattact ggcccctgct cgtgaccgac agccctgagt
     3661 ggcgcactac ccaggtcggc atcacggcct tcaaatcaag cgaagtggcc ctgttcaacc
     3721 tgcaaatggc gggcacgctc atcgtcatgc tgcccacttt gattctgctc gtggtcgggc
     3781 agcggcagtt ggtgcgcggc ctcacggcag gggcgctaaa gggctagagc ggcaaacaac
     3841 aacgcagaga ggagggcagc gggcggaatt gggcaacctt tgagctttac atggctcaaa
     3901 gagcgaacgt ctggcaattg aaacccatga ggaggaagga aatggcaatg acgcgaaatt
     3961 tccggcagat aatctccata tctttcatag tgatactact cacgagcatg ctggcggcct
     4021 gcggcggccc gacgaccact cccaccactg taagcaacac cgccaccacc gccccggcag
     4081 tcgatacagc cactacggca ccagccaacg acacgcccac caccgaagct atggcggaca
     4141 ccccgaccac tgcggctgtg gcacccacgg ccaccactgc gggggcttcg accacgatga
     4201 cggccaccac tgctcccgca cccaccgcca caccctatcc cacagtgcca gcggcggccg
     4261 gggctaccaa gattacgttc tggtttggcc tcaccggctt caacggcgga gtcacccagg
     4321 aagtggtcaa caggttcaac accagccaga ccaagtacta cgtcgaggct atccagcagc
     4381 ctaactacga cgacacgatc aacaagctaa gcaccagctt cgcgggcggc gcgctgccca
     4441 acgtcgtgca gatttacgac atcggcaccc agaggatgat agacagcaag cgtatcgtgc
     4501 ccgtgcagga cttcctggac aaagagaact cggacatcgc cgccgacctg gaaccggcgg
     4561 tcgccagcta ctacacggtg aacggcaagc tctactccat gccgttcaat agctctgcgc
     4621 ccgtcatgta ctacgacaag aacgccttca gagaggttgg cctggacccc gaaaagcccc
     4681 ctacaaccta cgacgagctg ttcgcggccg cgcagaagct ggtcaagaaa gacgcgagcg
     4741 gcaagatcgt ccgcagcggc gttgacttca ccctgtacgg ctggatactc gagcagcaac
     4801 tggcaatcca gactaccgtc tacgccgagc cggataacgg gcgcggcgcg gagcgggcca
     4861 ccagggtgaa cttcaacagc gaagccggcc agaactggct caacttcctc aagaagctac
     4921 aggacgaggg cgttggccgc agcctcgggc gccatagcgg cactaccaac ggcaccacgc
     4981 tgggggccaa tttctccagg ggggatgcag ccatcgcctt cgagagcatc gctaccctgc
     5041 gtaactggag caggcaggca gaggccgcag gcggcaaggt cgatgttggc gtggcgttcc
     5101 tgcccaagcc cacgggggca aaaggcggcg taatcatcgg tggtgcgtct ctctggatca
     5161 ccgaccaggg ttccgccgag cagcagcaag gggcctggga gttcgtgaag ttcacggccc
     5221 aacctgagac ccagtcgttc ttcgcttcca gcaccggcta ctatccgacg cgcagggccg
     5281 cctacgagct gcccgagatg aaagaggcgc tcgtccagtt cccgcagttc caggtagccg
     5341 tggacgagct ccgctcgacc gagccaagcc ctgccaccca gggtgcggtc tttggcacct
     5401 tcgcgggcac ccgcgtgctg gtggagggcg cgatggagca gttcatgctc ggtgaggccc
     5461 ctaccgcgaa agccgccctg gacgaagccg cggacgaggc gaacgaactg cttgaggaat
     5521 acacatccac gttgacaccg taaacccggt ccgcaacgct agctgaatat accccgtttg
     5581 ccggactcgg ctacgatgca ccggcaggcg gggtatattt aaccctgaga ggctatgcag
     5641 ttactatctc accgggaacg tgatgccgcc ctccacagga tggcgaacga aggagtcgat
     5701 atcctcgtcg tcggcggggg aatcacgggc gcaggggtgg cccttgacgc ggcggctcgc
     5761 ggttaccggg ttggcctggt ggagaagtcc gatttctcca gcggcacgag cagcaaatcg
     5821 accaagctgg tgcacggcgg gatacgctac ctggcccagt tcgattttgc cctggtacga
     5881 gagtctctgg tcgagcgcgg gcggctgatg cggaacgcgc cgttcctggt gcagcccgtc
     5941 ggctttctgc tgcccctgta cgccggtgcg aagcggccaa tgggcatgcc catacgtccc
     6001 ccgttcggcc tcggtatggg cctgttgctg cgtgcgggct tgctcgcgta tgacttgatg
     6061 tcgggccgac tgggtataaa gcggcaccgc cagattagca aagctaccgc cgcacgtctg
     6121 tacccatgcc tcaacacgtc taagctggag gctgctttca tctattacga tgcgcagact
     6181 gacgactcgc ggctgactgc caccgtgata ggcactgcgg ctcgacacgg cgcgctggtc
     6241 gccaaccacg ccgagctaaa aggcttccaa caagagaacg gaaagattac ggctgccctg
     6301 gtgcaagatg ggataagcgg acgtagcttc accatcccca ccggcaccgt cgtaaacgca
     6361 ggtggagctt ttgccgggcg catcgaagag ttggccggac cctccatgat taggatcaag
     6421 cccgccaaag gggtgcacct cacagtggag cgacaggcgc tgaagcttgg caggggcgct
     6481 atcgtgctgc cggagaccga cgataggcgt ctcctcttcc tggtgccctg gggggcgcac
     6541 gtcactatag gtaccacaga caccgagggc ggcgacatcg accggccgcg agccgaccag
     6601 gacgacgttg cctatctgct gcgtcacgtg aaccgctata tgcgctgcaa cctgcaagag
     6661 agcgacatca tcagcgcctg ggcgggctat aggtcgctgg tcacctcacg caaaagctca
     6721 aaagcctcgt cccgtttgtc tcgcacccat gcggtactgg atgggccagg gggtatggtg
     6781 accatcgtcg ggggcaagct tacaacctac cgccgcatgg cccaggacac gatagaccac
     6841 atagccaggc agcaaggcaa gcgtgtcgct cgtgccacgg agcgtctacc cctctcaggc
     6901 tccgaagatt ggcccagggc gcacgaggac ctcaacgcag cagccgcccg gtttcaactg
     6961 aagcccgaca cggcgcgccg cctggctatg tacggctcaa gcagccgggt tatcctggac
     7021 cttataacag ccgcgcccga ccttgccgcc cgcatcgtgc ccggcctgcc ctacataatg
     7081 gccgaggtag tgtatgcctg ccggtatgag atggccctcg acctggccga cctgttggag
     7141 cgacggctgc gtatcagctt cgaagaccgc tctcatggcg tgaaggtggc tcccagcata
     7201 gccgccttga tggcggagga gcttagctgg gacgaaacag aggtccaccg ccaggtgaat
     7261 aactatcgca atctcatagc cgaaagcttc cagtatggaa ccacagccgg cgaggccgaa
     7321 agggtagggg cgcatggccg ctaacgcaag tccggcaagc cggtacatcc tggcgctgga
     7381 ccagggcact accagctcgc gtgccatcat cttcgatcgc gcagggcacg tggtaggcca
     7441 atgcagccgc gaattcaggc agctttaccc gcagccgggg tgggtagagc acgaccctga
     7501 agagatttgg gccagccagc tcgaagttgc gcgcaaggtg ctgcgtgata gcgacatcgc
     7561 gcccggccgg atagcagcca taggggtcac caaccagagg gagaccaccc tcgcctggga
     7621 cagcgacacg ggcgagccta tctgcaacgc tatcgtctgg caggaccgca ggaccgcgca
     7681 gctttgcgac agcctgaagg aaagcggctg gggcagcaag atacaagcaa agaccggcct
     7741 cgtaatcgac ccgtacttca gcggcaccaa gctgaagtgg atactggaca acgtgcccgg
     7801 cgccgcgcaa agtgccgcaa gtggcaagct ccggttcggg acggtcgata cgttcctggt
     7861 ctggcgcatg agcgggggca gcctttacat aacggattac tcgaatgcca gccgcaccat
     7921 gctcttcaac atacacgagc tgcgctggga tgaagagata ctcgccgggc tggacatccc
     7981 ggcctccata ctgcccaccg tctaccccag cagccatttg tatggctacg cagccgaaag
     8041 tttcctgggt gagaaaatcc ccatcaccgg cgtggcgggc gaccagcaag cagccacctt
     8101 cgggcaggct tgttactctc cgggcatggc gaagaatacc tacggcacgg gctgcttctt
     8161 gctgatgaac accgggcgag tagccgtacc gagcaagcac aatttgctca ctacagtagg
     8221 ctggggcctc gggggagaga ttacctattg cctggaaggc tcggtattca gcgcgggcgc
     8281 ggtagtacag tacctgcgcg acagcttgcg attaatagat gatgccgccc aaacggagac
     8341 gttggccctt tcggtggaga gcagcggtgg cgtctatttc gtgcctgcct tcgtgggcat
     8401 gggcgctccc tactgggacc cctatgctcg cggggccgta ctggggctga ccaggggtag
     8461 cgggcgcgcc gagatagtgc gggccgccct ggagtccgtg gcctaccaga cgcgcgacgt
     8521 gctggaggct atggttgccg atagcgggga ggccctcacc gagttacgcg tggatggggg
     8581 gatggtggtg aataacttcc tcatgcagtt ccaggccgac atcctgggcg tgccggtcgt
     8641 gcgctccgcg ataggcgaaa ctacggccct gggcgctgcc tacctggccg gacttgcggt
     8701 cgattactgg cagagtgagg aagagatagc ccggaactgg tccgccgctc aagtgtacga
     8761 acctcgaatc gatcccgcct cgcgcgaggc gctctaccgc aactgggggc gggccgtaga
     8821 acgttccctg cgatgggagc aaccttgatc ttagggggcg aaactgcctc caattctacc
     8881 cggcctgttc aagcacaaga ccgccgcc
//
LOCUS       DASRWZ010000039         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_413664, whole genome shotgun sequence.
ACCESSION   DASRWZ010000039 DASRWZ010000000
VERSION     DASRWZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413664"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFH60_00525"
     CDS             <1..529
                     /locus_tag="VFH60_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6312291.1"
                     /translation="TLFLRHHAGVYRLLYGLVRVREWAEDLAQDTFLELYRNPPRGYE
                     GSSLLAWLCRVALNRGYNSLKGEDRSRQRAERVEGLARVTTSAEHSDPAVEVLRAEES
                     KRVREVLARLPERQGRLLLLRYAGLSYAECAGALEVAPGSIGTLLVRAEKAFREAYGS
                     WEEAGQSGLLERTVK"
     gene            526..2745
                     /locus_tag="VFH60_00530"
     CDS             526..2745
                     /locus_tag="VFH60_00530"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET6312292.1"
                     /translation="MTGNTGSAVRGELHDEGQLRAYLDGELPRLEAARVEVHLESCAE
                     CRERLEEIRTGAIEVSGLLGMPVHTPDPHVALSGLRQALGEEAVGLNPVTEGAAQALP
                     ASSAWSRERAHDSSGWRWSRLARSGVGVMLAAAVVLGLGLFWSGLPGRVLIKPPVLGI
                     PGSIEATSTAQAGEAAGVAVTPQNELETARRLIGLPAPDADLLDLSNGQTVKLSAFKG
                     RAVVLTFWETSCASCRAILQTLQETRDSLGPAGDVEFLSVTFSHANRDTPERVREFLK
                     DKPYTWRFLHDTTGDASQQYKVTAFPMTFVIDKSGIVRDVRVGTGDGLADLLVDSLTS
                     MSGAADRTPGTRDRIADFVPAGKVRHIVVTLRNESDVPTDPTRIVSRDIWFANGDNHL
                     LMRYTADQTDWSLVPVEPGISVYGAAPREVWLTDDALYVHKTGEPGPSPAANVVLKYP
                     YDPDYLTTYGPNPDALADLQKRYPEAQVVENTVLDGHPVVKVLHRAGTGQAPAAGTPG
                     SNSQSPFARTTTYWIDTETGQYRRVEVVTQAEAAQGNAMQTFTGTLRIERDEVLGAEQ
                     AAPGYFEFKLPEGATFIERERKVISFGMEEGAGGWSEHLDVLGNFSVLMPRTPDYSFD
                     GYGQTTLGAKEGSVTYGVKYSTLATGPLTPADPALLERTFELARAEGRVLSQQDITLG
                     AYPGREYRLTDANGNYRIWRVYVAGQRVYTVFAIAPDEATEAETIRDFFASFHLLNP"
BASE COUNT          533 a          881 c          948 g          460 t
ORIGIN      
        1 gaccctcttc ctgcgtcacc acgccggggt gtaccgcctg ctgtacgggc tggtgcgcgt
       61 gcgcgaatgg gctgaagacc tggcccagga cactttcctg gagctatacc gcaacccgcc
      121 gcgcgggtac gagggcagca gcttgctcgc ctggctgtgc cgcgtggccc tcaaccgggg
      181 ctacaactcg ctcaagggcg aggaccgcag caggcagcgt gccgagcgtg tggagggcct
      241 ggcacgcgtg actacctccg ccgagcactc cgatccggcg gtcgaggtgt tgcgggccga
      301 ggagagcaaa cgggtgcgtg aggtgctggc gcggttgccg gagcgccagg gcaggctttt
      361 gctgctgcgt tacgcgggcc tctcctatgc cgagtgcgcc ggagcgttgg aagtggctcc
      421 aggctcgata ggtacgctgc tggtacgcgc cgagaaagcg tttcgcgagg cgtacggctc
      481 gtgggaagag gcggggcagt ccggcctgct tgaaaggaca gtcaaatgac ggggaacacc
      541 ggtagcgccg tacgcggcga actgcacgac gaggggcaac tgcgcgcgta cctggacggc
      601 gagctaccca ggctcgaagc ggccagggta gaggtccacc ttgaaagctg cgcggaatgt
      661 cgcgagcgat tggaagaaat acgtaccggg gccatagagg tgagcggctt gcttggcatg
      721 cccgtgcaca cgcccgaccc ccatgtcgcg ctgtccggcc tgcgccaagc tcttggcgag
      781 gaggctgtgg gcctcaaccc agtcacagag ggggccgcac aggcactgcc cgcctcatca
      841 gcctggtcgc gggagagggc gcacgactcc tccgggtggc gctggtcccg gctggctcgg
      901 agcggcgtgg gtgtaatgct ggcggcggca gtggtgctgg ggctggggct gttctggagc
      961 gggctgccgg gccgcgtgct gattaagccc ccggtgctgg gtatacccgg ctcaatcgaa
     1021 gcgacctcga ccgcccaagc aggagaggcg gcaggagttg ccgtcacccc gcagaacgaa
     1081 ctggagaccg cacgcaggct gataggcctc ccggctccgg acgccgattt gctagacctg
     1141 tcgaacgggc agacggtgaa gctgtccgcg ttcaagggca gggccgttgt gcttaccttc
     1201 tgggaaacca gttgtgcgtc gtgccgcgct atcctccaga cgctacaaga aacgcgagac
     1261 agccttggac ccgccggcga cgtagagttc ctcagcgtta ccttcagtca cgccaacagg
     1321 gacacgcccg aaagggtgag agagttcctc aaggacaagc cctatacctg gcgcttcctc
     1381 cacgacacca cgggggatgc aagccagcaa tacaaggtta ccgctttccc catgaccttt
     1441 gtcatagaca agtcgggcat agtgcgagac gtgcgagtgg ggaccggcga tggtctggcc
     1501 gacctgctgg tcgacagcct gacgagtatg tcgggggccg ctgaccggac gcccggcact
     1561 cgtgaccgca tcgcggactt cgtgccggcc gggaaggtgc gccacatagt tgtcaccttg
     1621 cgcaacgagt ctgacgtgcc tacggaccct actcgaatcg tgtcgaggga catctggttc
     1681 gcgaacggcg acaatcactt gctgatgcgc tacaccgccg accagaccga ctggtcgctt
     1741 gtgccggtag agccggggat ctctgtctat ggcgcggccc ctcgcgaagt gtggctgacg
     1801 gacgacgccc tgtacgtgca taagaccgga gaaccggggc cttctccggc tgcgaacgtc
     1861 gtgctgaagt acccctatga cccggactac ctgaccacgt acgggccgaa ccctgacgcc
     1921 ctggcagacc tgcaaaagag gtacccggaa gcccaggtcg ttgagaacac cgtgctggac
     1981 ggccaccccg tggtaaaggt actgcaccgc gccgggacag ggcaagctcc ggcagcgggc
     2041 acgcccggct cgaactcaca gtctccgttc gcccggacta cgacctattg gatagacaca
     2101 gagacagggc agtacaggcg ggtggaagtg gtcactcaag ccgaggcggc ccagggcaat
     2161 gcgatgcaga cgtttaccgg cacactgcga atcgagcggg acgaggtttt gggcgcggag
     2221 caagcggcac ctgggtactt cgagttcaag ctgcctgagg gtgccacgtt tatagagcgt
     2281 gagcgcaagg ttatctcgtt cggcatggaa gagggggctg gcggctggtc cgagcacctg
     2341 gacgtgctgg gcaacttctc cgtgctgatg ccccgcaccc cggactacag cttcgacggg
     2401 tatggtcaga ctacattagg ggcaaaggag ggcagtgtca cgtacggcgt caagtactcc
     2461 accctcgcga caggccccct cacacctgcc gaccccgccc tgctggaacg gacctttgag
     2521 ctggctcgtg ccgaaggtag ggtgctctcg cagcaggaca ttaccctggg cgcgtacccc
     2581 ggcagagagt accggcttac ggacgccaac ggcaactacc gcatctggcg ggtgtacgta
     2641 gccgggcagc gcgtctacac cgtcttcgcc atagcccccg acgaggccac cgaagccgag
     2701 accatccgcg acttcttcgc ctccttccat ctcctgaacc cctagccccc tcccgtatcc
     2761 cgtaaatcgt tgcggcggga cgtaagttac aaaggagacg aaaacgcaaa gacgcaaaga
     2821 cg
//
LOCUS       DASRWZ010000040         4335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_482250, whole genome shotgun sequence.
ACCESSION   DASRWZ010000040 DASRWZ010000000
VERSION     DASRWZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482250"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..4074)
                     /locus_tag="VFH60_00535"
     CDS             complement(331..4074)
                     /locus_tag="VFH60_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6312293.1"
                     /translation="MNCPSCQTATQEGAKFCINCGNPLPISCSNCGTQLPPGAKFCHN
                     CGQAVGVAAAVAPPAPAAVAPPAPAVDPTLARLEQYIPKELLNKLELARASRSMEGER
                     RIVTVLFCDVKGSTMLAEQLDPEEWAEIMNGAFKYLIEPVYRYEGTLARLMGDAILAF
                     FGAPIAHEDDPQRAVLAALDIVEGIKEYRQQIERSYGFDLSVRVGINTGLVVVGEVGS
                     DLRVEYTAMGDAVNVAARMEQTAEPGSVQISGNTYKSVSNLFELEALGGIEVKGKSEP
                     VEAYRVLARREGALPTRGIEGLNSPLVGRDRELQTLRARMDDLMAGRGQIVSVVGEAG
                     LGKSRLISELRKALIAEGVMVEGGREPEDLQTIPCIQWCEGRSLSYETTTPYAPFVDM
                     FNSCFGLHTGDSSRDRLEKVRVNVGRVMPGQEEMVVPFLATLLGIELEGEDAERVRYL
                     QPPQVRGRIFFTVQSYIAALASKTPLVLMFEDLHWVDPTSMELLTQLVPLTDSASLMI
                     LAAFRPNTQDPSWQFHESAAREYAHRYTHMAIEPLDEEDSRQLVANLLEIEDLPEAVR
                     ALILKKAEGNPFFVEEVIRSLLDMKLVVREGDRWRATREIENIAVPDTLAGVITARLD
                     RLDEESKRVAQTAAVVGRDFAYDALEEVYEAPHALGGAVSTLQRRELIREKSRIPRRA
                     YLFKHVLTQETAYASLLLSKRRDLHRRVGEYLERVEPERAGEIARHYLEARQEERALP
                     YLVQAGDSAGLADAREEARGYYRKALDILQRVPDGALARRAYEGLGKVLEFSMDVPGA
                     LGAYQEMLDYGEEQNDVPMQISALNKLSYANAFMMGQFEEADAQLAAAEQLANQHQDL
                     AGLAEMYTVKCGMCTAMADFSNANKYLTEAAELGRKLDLKDQTAYGLAHKANTLVYMA
                     QFEEAWKTAQEGLQVAAEAGNRERRAEILANSLPFIYLHRGELDAAYMSAEQGYKIGT
                     QIGAMFSPVFASYLLGTVAFMQGDYEDAMRWQERGLEGAQMLMSVMPFMAVLPLGSLG
                     TICLGISEKLKDRTVQYHTQALEILQTPVGAMTGATGYSDLGFCALELGYADLAADYF
                     ERGLNQPSTTMYLMRPLLLAGSGLAALARKQPEEAVSRVSMARAYAREHEMMHFYPHI
                     ELMAGQVSAGRGEYEAALEQLNRAEDLAMDMRMRPIVWQARAGAAHALSVLGHEGEAA
                     EKRQGAAEMIEEIAALFKSEEYRALFLESAHKKLQARVGEAVA"
BASE COUNT          690 a         1534 c         1280 g          831 t
ORIGIN      
        1 gccagccagg gtgggtgagg cggttgcgta gcggcgggcg gcacagagcg gcacagcagc
       61 acgtcgatgc gtggagggac gggcagaggt taacaggacc aagagtggcc ctgtcatcct
      121 gagcgtagcg aaggatctaa agaaggtggt atacggatgc tgttgaacgt tgcgtcgtgt
      181 cgtacggtgc tggcattcag tgtgttagta ttgccagcat cttagatcct tcgctacgct
      241 cagggtgaca gggtcactct tggtcctgtt aacctccgcc tgtcccttca cgcatcgacg
      301 tgctgctgtg ccactctgtg tcgcccgccg ctacgcaacc gcctcgccca ccctggcttg
      361 cagcttctta tgcgcgctct ccaggaacaa ggcgcggtac tcctcactct tgaacagcgc
      421 cgcgatctcc tcgatcatct cggcggcccc ctggcgcttc tcggcggctt ccccctcgtg
      481 gcccaggacg gagagcgcgt gtgcggcacc ggcgcgggcc tgccacacta tggggcgcat
      541 tcgcatgtcc atcgccaaat cctcggctcg gttgagctgc tccaacgcgg cctcgtactc
      601 gcccctgccc gcgctcacct gccccgccat aagctctatg tgcgggtaga agtgcatcat
      661 ctcgtgctcg cgggcgtagg cgcgggccat gctcacccgg ctgacggctt cctccggctg
      721 cttgcgcgcc agggccgcca ggccgctacc cgccagcaac aagggacgca tcaggtacat
      781 cgtcgtagag ggctggttca ggccgcgctc gaagtagtcg gccgcgaggt cggcgtagcc
      841 aagctccagg gcgcaaaagc cgaggtccga gtagccggta gcccccgtca tcgccccaac
      901 cggcgtttgc agtatctcca acgcctgcgt gtggtactgc accgtgcggt ccttgagctt
      961 ctcgcttatg cccaggcaaa tcgtgccaag cgaccccagc ggcagcaccg ccatgaatgg
     1021 catgacgctc atcagcatct gcgcgccctc caaaccgcgc tcctgccacc tcatcgcatc
     1081 ctcgtagtca ccctgcatga acgccaccgt ccccagtaag tagctggcga acaccgggct
     1141 gaacatcgcc cctatctgtg tgccaatctt gtacccctgc tccgcgctca tgtaagccgc
     1201 gtccagttcg cccctgtgca ggtaaatgaa cggcagggag ttagccagta tctccgccct
     1261 gcgctcccgg ttgcccgctt ccgccgctac ctgcaagccc tcctgggcgg tcttccacgc
     1321 ctcctcgaac tgggccatgt agacgagcgt gttcgccttg tgcgccagac cgtaggccgt
     1381 ctggtccttc aaatcgagct tgcgtccaag ctcggcggcc tcggttagat acttgttggc
     1441 gttgctgaaa tcagccatcg cggtgcacat gccgcacttc accgtgtaca tctccgccag
     1501 tcccgccagg tcctggtgct ggttcgccag ttgctcggct gccgccaact gcgcgtcggc
     1561 ctcctcgaac tgccccatca taaaggcgtt ggcgtatgac agcttgttga gcgccgaaat
     1621 ctgcatcggc acgtcgtttt gctcttcccc gtagtccagc atctcctggt aggcgcccaa
     1681 cgcgcccggc acgtccatcg agaactcaag caccttgccc agcccttcgt aggcccgcct
     1741 cgccagcgcc ccgtccggca ctctttgcag gatgtcgagc gccttgcggt agtaacccct
     1801 ggcctcctcg cgggcgtcgg ccagccccgc gctgtccccg gcttgcacca ggtacggcag
     1861 ggcgcgctcc tcctgcctgg cctccaggta atgccgggct atctcgcccg cgcgctccgg
     1921 ctccacccgt tccaggtact cgcccactcg cctgtgcagg tcgcgccgct tgctcagcag
     1981 cagcgaggcg taagcggttt cctgcgtcag cacgtgcttg aacaggtacg cgcggcgggg
     2041 tatgcggctc ttttcgcgta taagctccct gcgctgcaag gtggacaccg cgccgcccag
     2101 cgcatggggc gcttcgtaca cttcttccag cgcgtcatac gcaaaatcgc gccccaccac
     2161 cgccgccgtc tgcgccaccc gcttcgactc ctcgtccagc ctatcgaggc gggccgtgat
     2221 gaccccggcg agcgtgtcgg gcacggcgat gttctctatc tcccgcgtgg cgcgccagcg
     2281 gtcgccctcc cgcaccacca gcttcatgtc cagcagcgag cgtatgacct cctccacgaa
     2341 gaaggggttg ccctcggcct tcttgaggat gagggcgcgc accgcctccg gcaggtcctc
     2401 tatttccagc aaattagcca ctagctgccg cgagtcctct tcgtccagcg gctctatcgc
     2461 catgtgcgtg tagcggtggg cgtactcgcg ggccgcgctc tcgtggaact gccacgaggg
     2521 gtcctgcgtg ttggggcgga aggcggccag tatcatcagc gaggcgctgt cggtgagggg
     2581 cacaagctgg gtaagcagct ccatcgaagt ggggtccacc cagtgcaggt cctcgaacat
     2641 cagcaccagg ggcgtcttgc tcgcgagcgc ggcgatgtac gactgcacgg tgaagaagat
     2701 gcggcccctt acctggggcg gttgcaggta gcgcacccgc tccgcgtcct ccccctccag
     2761 ctctataccc agcagcgttg ccaggaacgg cacgaccatc tcttcctgcc ccggcatgac
     2821 ccgccccacg tttacccgta ccttttccag cctgtcccgc gagctatcgc cggtgtgcag
     2881 gccgaagcat gagttgaaca tatccacgaa gggcgcgtaa ggcgtggtcg tctcgtacga
     2941 gagggagcgg ccttcgcacc actggatgca gggtatcgtc tgcaagtcct ccggctcgcg
     3001 ccccccctcc accatcacgc cctctgcgat gagcgccttg cgcagctccg atatgaggcg
     3061 cgacttgccc agccccgcct cgcccacgac cgatactatc tgcccccgcc ccgccataag
     3121 gtcgtccatg cgcgccctga gcgtctgaag ctccctgtcg cgccccacca gcggcgagtt
     3181 cagcccctcg atgcctcgcg tgggcaaagc cccctcccgc cgggcgagca ccctgtaagc
     3241 ctccaccggc tccgacttgc ccttgacctc tatcccgccc agcgcctcta actcgaacag
     3301 gttcgatacc gacttgtagg tgttgcccga aatttgcacg ctgccaggct cggccgtctg
     3361 ctccatgcgc gcggccacgt tcacagcgtc gcccatcgcc gtgtactcca cccgcaggtc
     3421 cgacccgacc tcccccacga ccaccagccc ggtgttgatg cccacccgca cgctcaggtc
     3481 gaagccgtag ctacgctcga tctgctgccg gtactccttg atgccctcca cgatgtccaa
     3541 cgccgcgagc accgcccgct gggggtcgtc ctcgtgcgcg atgggtgccc caaagaaggc
     3601 cagtatagcg tctcccatga ggcgggccag cgtgccctcg taccggtaca ccggctcgat
     3661 caggtacttg aacgccccgt tcatgatctc ggcccactct tccgggtcga gttgctcggc
     3721 cagcatggtg gagcctttga cgtcgcaaaa gagcaccgtg acgatgcgcc gctccccctc
     3781 catgctgcgg ctggccctcg ccagctccag cttgttgagc aactccttcg ggatgtactg
     3841 ctccagccgc gccagggtgg ggtcaacggc tggcgcgggg ggtgcgaccg cagctggtgc
     3901 cgggggtgcg accgcagccg cgacgcccac cgcctgcccg cagttgtggc agaacttcgc
     3961 acctgggggc agttgcgtac cgcagttgct acagcttatc ggcagggggt tgccgcagtt
     4021 aatgcagaat ttggcccctt cctgggtggc ggtttgacaa ctagggcaat tcatacatgg
     4081 ctccttattc cgtcccaacg gggctagtct ttagagcgaa cgtacaggat tgttcttacg
     4141 gtgttggtgg aggtaaagag gcgggaggag cggcaggccc gcgccccggt tcgccactaa
     4201 gcgagataga cccggatgca tagcgcatcg agcggcgggg aatattgggg gcctgaatgc
     4261 tgacattcta tcacacctcg ctacaaaagg atacccactc ctcgcgccgt ccggagcttg
     4321 aggctgagcg cgtac
//
LOCUS       DASRWZ010000041         3762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1099081, whole genome shotgun sequence.
ACCESSION   DASRWZ010000041 DASRWZ010000000
VERSION     DASRWZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099081"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..>3762
                     /locus_tag="VFH60_00540"
     CDS             206..>3762
                     /locus_tag="VFH60_00540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET6312294.1"
                     /translation="MPKDLVAAAHRLKMPALALTDRNNLYGAVHFYMQARKAGIKPVL
                     GMEVDLEDGSSLVLLARNMDGYRNLCHLSSALRLNADPEEFPPAGFGEEDEEISPWDP
                     GVWGVPLFGFTAKPPKSSRSNATRLSLTTNTNSKLKIQSSKLPLEVVFSGRHTRGLIA
                     LSGGSRGLLNRLLVSSQGRPRRAAKVAGMLLSAFGEGNFFVELTACTEEERRLIPTLA
                     SLAYDLGIPLAATNDVLYIEPGDATVAAALASARPGGRGRASDSGRLIEDEGIHHDPH
                     AQRLTAPSTGRHLASTEEMAALFEEYPQALANARFIADQCDVELPLHKPLFPAVDLGR
                     RGNETPFSRLWKLCFAGATRLYKPLDAGVISRLKHELEVIEKLGFSPYFLVVYDIALF
                     ARRKGIPTMARGSAANSLVAYVLGITQVDPLAHDLLFERFLNPSRAEFELPDIDIDLC
                     WRRRDEVLHYVYERYGRDHVAIVGTHITFRLRSAWREMAKALGIHPDRISRVASRLPH
                     LFTAEEILEENVPELEALGEDQEVRVYGVEETLLPGVLNEDQKFRDALERQAFELCKA
                     IEGLPRHAGMHCAGVVITPGPITDLVPLQRAARDASMSITQYEKDAIEAMGLVKMDLL
                     GSRALTTLVDSVQAAGLAKEARDVHDSLAAIPFDDAATYRMLAQGDTLGCFQLESPGM
                     RGLLKWLRPRGMNDMAAAISLFRPGPMEGGFLEIFMRRHLGQEPVSYPHPSMEPILKS
                     TYGIILYQEQFLRLGHILGGLSLGDAEKLRKEIGKSRSAEERARLGASFVAGCIERGI
                     DQMQAERVWEIISGYTGFGFCQAHAYSYALTAYRSAYMKAHHPAQYMAAQINNLGGYY
                     GTGVYVEDARRLGLELLPPHANLSGAFCEAGQPPDTRRIRFGLQFVKGLSEKTIALIQ
                     SDRRARGPFRHLLDLLSRVEMSPSEAASLVKVGACDDLGTGGEGPGTAESDTGELAPL
                     SELPPLNRRQMLWLLPTIISHNRPKRASTQHSKLITHNSPRSLAPIQLALGDLMHDVG
                     APRILGGTTLRIDVPDMPDFSLVEKLRLEREVLGFTLTRNERELYGDMVAARGIVPSH
                     ALGEYAGHKVTVAGVIAAGRRHLAKGGEWMLFVSLQDSGGLMEVVLFPEVYKEHAALI
                     ANNGCGPYLVTGTVQVAGKGRGVGVQLPVGVRPSDATVL"
BASE COUNT          700 a         1229 c         1193 g          640 t
ORIGIN      
        1 ccaagaagag tgtcccaggc tctttgctcc gagaaagggg agggaaatat tagccattga
       61 ctagaacata tgttctatgg tatgtatatc ttacgcactg cgcgagcagg gcaggaggca
      121 cccgcacgag gcactcgaca gaggtcacat gggccaggta catcttcact gccattccgc
      181 atactcactc ctggaaggta cggctatgcc gaaagacctg gtcgccgccg cccaccgcct
      241 caagatgccc gcgctagctc tcaccgaccg caacaacctg tacggggcgg tccacttcta
      301 tatgcaggcg cggaaagcgg gtataaagcc cgtgctcggc atggaggtgg acctggaaga
      361 cggctccagc ctggtgctgc tggcccgtaa catggacggc taccgtaacc tgtgccacct
      421 atcgtcggcc ctgcgcctca acgccgaccc ggaagagttc ccacccgcgg gcttcggcga
      481 agaagacgaa gaaatctcgc cctgggatcc tggcgtgtgg ggtgtgccct tgttcgggtt
      541 caccgccaaa cctcccaagt catctcgctc gaacgccacc aggctatctc tcaccaccaa
      601 cactaactca aaactcaaga ttcaaagctc aaaactcccc ctggaggtag tgttctccgg
      661 caggcacaca cgcggcctta tagccctctc aggcggctcc aggggcctgc tcaaccggct
      721 gctggtatca tcgcagggga ggccgcgcag ggcggccaag gtggcgggca tgttgctgtc
      781 ggccttcggg gagggcaact tcttcgtgga actgaccgcc tgcaccgagg aggaacgccg
      841 cctcataccc accctggctt ctctcgccta cgacctgggt atccccctgg ccgccaccaa
      901 cgacgtgctc tatatcgagc cgggcgatgc cacggtcgcg gcggcgctgg cctccgcccg
      961 gcccggtggc aggggtaggg cgtctgatag cggcaggttg atagaggacg aaggcatcca
     1021 ccacgacccg cacgctcagc gtctcacagc cccgtccacg ggccgccacc tcgcctccac
     1081 ggaggagatg gccgccctgt tcgaggaata cccgcaggcg ctcgccaatg cccgcttcat
     1141 cgcggaccag tgcgacgtgg agttgccgct gcacaagccg ctctttccag ctgtggacct
     1201 ggggcggcgc ggcaacgaaa cgcctttctc gcggctctgg aagctgtgct tcgcgggggc
     1261 tacccgactt tacaagccgc tcgacgcggg ggtcatatcc cggctgaaac acgaacttga
     1321 ggtaatagag aagctggggt tctcgcccta cttcctggta gtgtacgaca tcgccctgtt
     1381 cgcccggcgc aagggcatac ccaccatggc gcgaggctct gccgccaaca gcctggtggc
     1441 ctatgtgctc ggcatcaccc aggtggaccc actcgcccac gacctgctgt tcgagcggtt
     1501 cctcaacccc tcccgcgccg agttcgagct gcccgatata gacatagacc tatgctggcg
     1561 gcggcgcgac gaggtgctgc actacgtcta cgagcgctac ggccgcgacc acgtagccat
     1621 tgtgggcacc cacatcacct tccgcctgcg ctcggcatgg cgcgagatgg caaaggcact
     1681 gggtatccac cccgaccgca tcagccgcgt tgcctcgcgc ctcccgcacc tcttcaccgc
     1741 agaagaaatc ttagaggaga acgtgcccga actggaagcg ctcggtgagg accaggaagt
     1801 cagggtatat ggcgtagagg agaccttgct gccgggtgtg ctcaacgaag accagaagtt
     1861 cagggatgcg ctggaacgac aggccttcga gttgtgcaag gctatagagg gcttaccacg
     1921 ccatgccggt atgcactgcg ctggggtagt catcacgccc ggacctatca ctgacctggt
     1981 gcccctgcaa cgggcggccc gcgacgccag catgtcgatt acccagtacg agaaggacgc
     2041 tatcgaggcc atgggcctgg tgaagatgga cctattagga tcgcgggcgc tcaccacgct
     2101 ggtcgattcg gtgcaggcgg cgggactggc gaaggaggcc cgcgacgtgc acgacagtct
     2161 cgccgctatc ccctttgacg acgcggccac ctaccgcatg ctggcgcagg gggacacgtt
     2221 gggctgcttc cagctggaga gtccgggcat gcggggcctg ctcaagtggc tgcggccccg
     2281 gggcatgaac gacatggccg ccgctatctc gctgttccgc cccggcccga tggagggcgg
     2341 attcctggag atattcatgc gcaggcattt gggccaggag ccggtatcgt acccgcaccc
     2401 ttcgatggag cctattctca agagcaccta cggcattatc ctctaccagg agcaattcct
     2461 gcggctgggg catatcctgg gcgggctgtc gctgggagac gccgagaagc tgcgcaagga
     2521 gataggcaag agccgctctg cggaggagcg ggcgcggctg ggggccagct tcgtggctgg
     2581 gtgcatcgag cggggcatag accagatgca ggccgagcgg gtgtgggaga tcatatcggg
     2641 ctacaccggc ttcggcttct gccaggccca cgcctactct tacgccctca ctgcttaccg
     2701 ctcggcctac atgaaggcgc accacccggc gcaatacatg gccgcgcaga tcaacaacct
     2761 gggcggctac tacggcaccg gcgtatacgt ggaggacgcc cgccgcctcg gcctggagct
     2821 gctgccgcca cacgccaacc tgagtggggc gttctgcgag gcgggccagc ctcccgacac
     2881 ccggcgtatc aggttcggct tgcagttcgt caagggcctc tccgagaaga ctattgctct
     2941 catccagtcg gatcgacgcg cccgcggccc gttccggcac ctgctcgacc tgctctcccg
     3001 cgtcgagatg tcgccctcgg aggccgcttc cctggtcaag gtaggcgcgt gcgacgacct
     3061 gggaaccggg ggcgaggggc cgggtaccgc cgagagtgac acaggggagc tagcgccgtt
     3121 atccgagctg ccgcccctca accgcaggca gatgctatgg ctccttccca ccatcatctc
     3181 ccacaacaga cccaaacgcg cctcaactca acactcaaaa ctcataactc ataactcccc
     3241 caggtcgctt gcccccatcc aactggccct gggcgacctc atgcacgacg taggcgctcc
     3301 ccgcatcctc ggcggcacca ccctgcgcat cgatgtgccc gacatgcccg acttcagcct
     3361 ggtcgagaag ctgcgcctgg agcgcgaggt gctaggcttc acgcttacgc gcaacgagag
     3421 ggagctatat ggcgatatgg tagccgcgag ggggatcgtg ccgagccatg ccctgggcga
     3481 gtacgcgggg cacaaggtaa cggtagcggg ggtgatagcg gccggtcgcc ggcacctggc
     3541 gaagggtggg gagtggatgc tgttcgtgtc gctgcaagac tccggcggtc tgatggaggt
     3601 ggtgctcttc ccggaggtct acaaggagca cgcggcgctc atcgccaaca acggctgcgg
     3661 tccgtacctg gtcacgggca cggtgcaggt ggcgggcaag ggcagggggg taggagtgca
     3721 gcttccggtg ggggtgcggc cgtccgacgc gacggtgcta aa
//
LOCUS       DASRWZ010000042         4932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1167740, whole genome shotgun sequence.
ACCESSION   DASRWZ010000042 DASRWZ010000000
VERSION     DASRWZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167740"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="VFH60_00545"
     CDS             <1..1575
                     /locus_tag="VFH60_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HET6312295.1"
                     /translation="AKQPLGTDDAKRRPISYTFEIVSPKDDSIYAALRPFSFDTSSIL
                     ELSWRDLDEVYNIDRFYDKENPRSINEVPLEMRKLIGVLNQLQKELQGTAPAEGVDED
                     IEARLASMPSALEVEAQRQELARRGIHFSIDVSPGPNYTVNLRAQGQIPVYDDISSVH
                     APQDLSRGDSYSALSLVSEATDEELRLASTDYPAWVRDRYLSLPVSLDPAVRELGANI
                     VQDAGANNPLDAARAIEAHLRENYEYSTSIGQPPQGRDRVAWFLFENKRGYCEYYASA
                     MIVMLRSLDIPARFAAGYSPGSYDTKEKQFVVRESAAHAWPEVYFPGYGWIQFEPTPS
                     QPVRSEAIQPAEEPTPFGEAAPEPTITVAAPDPDRGLGEDEEQPTGGAGGGGFTLPWD
                     LGGPGGGWILLSLLAGGAGLGAVFFVRRKPNTAASPGVYYGKLLSLSRFLRLGPAAHQ
                     TPYEFSETLGREFPGTSSLARTISRGYVRERFSPRPADHTERVAVQKAWDSLRGRLVR
                     SLPTRQVRGWVSRRKR"
     gene            1779..3404
                     /locus_tag="VFH60_00550"
     CDS             1779..3404
                     /locus_tag="VFH60_00550"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HET6312296.1"
                     /translation="MANRVTTIPNKRVAPEKVPVPKSSGGGLADAQGRQRSDLVETVW
                     ALFCSVRFAVVLNVALALAAMIGTVIPQMPAGIQNFDQELKVFLDNAQSRYGDLSGLL
                     HWAGFYDLYYSLWFRLLVVMVVFSIIMCTLNRWQPIMRQIRTPSLRFSDGFISGLSEK
                     AEFRSVPVDVATAEQALRGALRKGRYRTVAEASEDGSKVYVYADRDRWSKLVTFVSHA
                     ALVMLILVWAGLANFGWREQSVYFYPGQEVNVGHDTNFSVRNDKFWIEYYADGKTIKE
                     YKHTLAVVQDGKDVLTKTIVVNDPLRYQDINFFLVSYQPVMYVKATDAAGKELEARKM
                     TASGPISATTGTAGALVEFDQVSSDNLPRDIVQYPVGDRFLTLSLVYYQDVGRLENEN
                     PPVYAQVFVDREFETPIYDGFLPRTGPLKLPGFEDINFTFTRDTATVLEVAKDPGLGL
                     VGFFFVVMAAGFTLSMYTSFTRCWARIGVDETRPGTVNLLVGGLAEKNKVSFERDFER
                     LALRLKENLAKAAREAALVRPSEAPRAAETVEV"
     gene            3441..4565
                     /gene="ccsB"
                     /locus_tag="VFH60_00555"
     CDS             3441..4565
                     /gene="ccsB"
                     /locus_tag="VFH60_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HET6312297.1"
                     /translation="MGFDAPTFGFNEPTLTIVAYLGYLFAFLFFCAHLMTRSGRALLA
                     PRSQVTLAGAGAGAGVSGSMDIDIDGQAEGSARGVSYSPVLGRIGSGLVLFAWLSLGT
                     ALAMRWVHAGHPPYVTLYEIATMLVFGITTVYLVLFEGVLKTRALGAFVVLLIFCLHS
                     YAVWFIPTDLKRTIELVPALRSNWLLIHVSMAIISYSAFAAAAGAGLTYLAKHHLKGR
                     IAAALPSGAAIEEFMYRAVAVGFPFVTLVLITGAIWAQEAWTKAWSWDPKEVWALITW
                     LTYAAYLHVRVQRGVRNVTMAWLSLIGFAVVMFTFMGVNYLVEWFGLQSMHTYSIDSG
                     RPPGLSVASIGTVVVFVVLLLLAFVSWLRVKFPGKRSPRK"
     gene            4573..>4932
                     /locus_tag="VFH60_00560"
     CDS             4573..>4932
                     /locus_tag="VFH60_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312298.1"
                     /translation="MAQAITANRGTLKAHFWAGIVLLALGHVLIFATGPSGEHLRPLS
                     DYWFAAVWFGYIFALDALVYRRDGRSLFVNHRKVFLWMLPLSALVWLGFEGVNWFVKN
                     WNYLRPFDIPEWWAQLWS"
BASE COUNT          853 a         1490 c         1605 g          984 t
ORIGIN      
        1 gccaagcagc ctcttggcac ggacgatgcc aagcgcaggc cgataagcta cacgttcgag
       61 atagtcagcc ccaaggacga cagcatctac gcggccttga ggccgttcag cttcgacacc
      121 tcgagcattc tcgaactttc ctggcgcgac ctggacgaag tgtataacat agaccgcttc
      181 tatgataagg agaacccccg gagcatcaac gaggtgccgc tggagatgcg caagctgata
      241 ggcgtattga accagttgca gaaggagttg caaggcaccg cgcccgctga gggggtggac
      301 gaagacatcg aggcgcgcct ggcgtcgatg ccaagcgccc tagaggtgga agcgcagcgc
      361 caggagcttg cgaggcgcgg catccatttc agcatagacg tctcgccagg tccaaattac
      421 acggtcaacc tgcgtgcgca gggccagatt ccggtctacg acgacatatc gtccgtgcac
      481 gcgccgcagg acctgtcgag gggtgatagc tactcggcgt tgtcgctggt ctccgaggcc
      541 accgatgagg agcttcggct cgcctccacc gattacccgg cgtgggtgcg cgaccgctac
      601 ctgtcgctac ctgtgtcgct cgaccccgca gtgcgggagt taggcgcgaa catagtgcag
      661 gacgccggtg ccaacaaccc gttggatgcc gctcgcgcca tcgaggcgca cctgcgcgag
      721 aactacgaat acagcacgtc catcggccag ccgccgcagg ggcgtgacag ggtcgcctgg
      781 ttcctgttcg agaacaagag gggctactgc gaatactacg catcggccat gatcgtgatg
      841 ctgcgaagcc tggacatacc ggcccggttc gccgccggtt actcccccgg tagctacgat
      901 accaaagaga agcagttcgt ggtccgggag tcggcggcgc atgcctggcc cgaagtgtac
      961 ttccccggct acggctggat acagttcgag ccgacgccct cgcagcccgt ccgcagcgaa
     1021 gctatccagc cggcggaaga gccgaccccc tttggtgagg cggccccgga gccgactatc
     1081 acggtagcgg cacccgaccc ggatcgcggc ctgggtgagg acgaggagca acccacgggc
     1141 ggcgccggcg gcggtggatt tacgctcccc tgggacctgg gtgggcctgg cggtggttgg
     1201 atcttgttgt cgctgctggc cgggggcgca gggctgggcg cggtcttctt cgtgcgccgc
     1261 aagccgaata ccgctgccag ccccggcgtg tactatggca agctgctgtc gctgtccagg
     1321 ttcttgcggc tcggtcccgc ggcgcaccag acgccctacg agttcagcga gacgctgggg
     1381 cgcgagttcc ccggcacgtc ctcgcttgcc aggacgatct cgcgtggcta cgtacgcgag
     1441 aggttcagcc cccggcctgc cgaccacacc gagcgggtag cggtgcagaa ggcctgggat
     1501 tcgctacgtg gtcgcctcgt gcgcagcctg ccgacacggc aggtgcgcgg gtgggtctca
     1561 cgcaggaagc gataggcgta attgtgggga gcggggtcat gtgccccgct ccacctttcc
     1621 cccggtcgcg ccgggcacag cgttaaggca atattcatgc gcgactgctt gacggtgcat
     1681 atacctgatg gtaatatcaa gctggaatac cgtttctcgg tcaagcttcc gcgtgcctgc
     1741 tagtccctcg ctactttgct taatcagccg actttcatat ggcgaaccgc gtaacgacca
     1801 tacccaataa gcgtgtcgct cctgaaaagg tacccgtgcc caagagtagc ggcggtgggc
     1861 tggccgacgc acaggggcgg cagcgtagcg accttgtaga gacggtctgg gcgctctttt
     1921 gctcggtgcg gttcgcggta gtcctcaacg tggccctggc gctggcggcc atgatcggca
     1981 ctgtcatccc ccaaatgccc gccggcatcc agaacttcga ccaggagctg aaggtcttcc
     2041 tggacaacgc tcagtctcgc tacggcgacc tttcgggcct gttgcactgg gcaggtttct
     2101 acgatctcta ctactccctc tggttccgcc tgctggttgt gatggtggtg ttcagcatca
     2161 tcatgtgcac cctcaatcgc tggcagccca tcatgcgaca gatacgcact cccagcctgc
     2221 gcttctccga tggcttcata agcgggctgt ctgagaaggc cgagttccgt agcgtgccgg
     2281 tggatgttgc cacagcggag caggcgttac ggggagcatt gcgcaagggc cgctatcgca
     2341 cggtggccga agcctcggag gacggcagca aggtctacgt gtacgccgac cgggaccgct
     2401 ggtccaagct ggtcacgttc gtgagccacg ccgcgctggt catgctcata ctggtatggg
     2461 ctgggctggc gaacttcggc tggcgcgaac agtcggtcta cttctatccg gggcaggagg
     2521 tgaacgtcgg ccacgacact aacttctcgg tgcgcaacga caagttctgg atcgagtact
     2581 acgcggacgg caagacgatc aaggagtaca agcacaccct ggcggtggtc caggacggga
     2641 aggacgtgct gaccaagacc atcgtggtca acgacccact gcgctaccag gacatcaatt
     2701 tcttcctggt cagctaccag ccggtgatgt atgtgaaggc caccgacgcg gcgggcaagg
     2761 agcttgaagc ccgcaagatg accgccagcg gccccatctc agccaccacg ggcacggccg
     2821 gtgcgcttgt cgagttcgac caggtcagca gcgacaattt gcctcgcgac atcgtgcagt
     2881 acccggtagg cgaccgcttc cttactctgt cgctggtcta ctaccaggac gttggtcgcc
     2941 tggagaatga gaaccccccg gtgtacgccc aggtgtttgt ggacagggag ttcgagacgc
     3001 ccatctatga tggtttcctg ccgcgcacgg ggccgcttaa gctgccaggc ttcgaggaca
     3061 tcaacttcac ctttacgaga gataccgcca ccgtgctgga agtcgccaag gaccccggcc
     3121 tggggctggt gggattcttc tttgtcgtga tggcggcggg ctttacgctc tccatgtaca
     3181 ccagctttac ccgctgctgg gcgcgcattg gggtggatga gacgcggccc ggaacggtga
     3241 atttgctggt gggcggcctg gctgagaaga acaaggtgag tttcgagcgc gacttcgagc
     3301 gtttggccct gcgcctcaag gagaacctgg cgaaagccgc tcgtgaggcg gcactggtac
     3361 gtccctcgga agcgccgcgt gccgctgaaa cggtagaagt ataggctaaa cgttgctttc
     3421 cggtctgtgt aggaggagtt atgggtttcg acgcaccgac gttcggcttt aacgaaccga
     3481 cgctcacgat agtggcctat ctgggctacc tgtttgcctt cctgttcttt tgcgcgcacc
     3541 tcatgacgcg ctcaggccgg gcattactgg ctccacgcag ccaggtgacg ctcgcggggg
     3601 caggtgcggg cgcgggcgtg agtggcagca tggatataga cattgatggg caggctgaag
     3661 gctcggcgcg gggggtttcc tattcgccgg tgctgggccg catcggaagc gggctggtgc
     3721 ttttcgcctg gctatcgctg ggtaccgcgc tggcgatgcg gtgggtccac gccgggcacc
     3781 cgccttacgt caccctttat gagatcgcca ccatgctcgt gttcggcatc actacggtct
     3841 acctggtgct gttcgagggg gtgctgaaga cgcgcgccct tggggctttc gtggtgctcc
     3901 tcatcttttg cctgcacagt tacgcggtgt ggttcatacc gaccgacctg aagcgtacca
     3961 tcgagttagt acccgcgctc cggtccaact ggctccttat ccatgtgtcg atggcgatta
     4021 tctcctacag cgcctttgcc gccgccgccg gggcgggcct cacgtacctg gcgaagcacc
     4081 acctcaaggg caggatcgcc gcggctctac cgtcgggcgc ggcgatagag gagtttatgt
     4141 accgtgcggt ggcggtgggt ttccccttcg ttacgctggt gctgataacc ggggcgatat
     4201 gggcgcagga ggcctggacc aaagcctggt cgtgggaccc caaagaggta tgggcgctca
     4261 tcacctggct cacctacgct gcgtacctgc acgtgagggt gcagcgtggt gtgcgcaacg
     4321 tcacgatggc ctggctgtcg ctgatcgggt tcgcggtggt gatgttcacg ttcatgggcg
     4381 tcaactacct ggtcgagtgg ttcgggctac agtcgatgca cacctatagc atcgatagcg
     4441 gcagaccgcc cggcttgagc gtggccagta tcggcacggt ggttgtgttc gtggtgctcc
     4501 tgctgctggc gttcgttagc tggctgcgcg tgaagttccc cggcaagcgc tctcccagga
     4561 agtagcgaat acttggcgca agcaattacg gccaaccggg gcacgttgaa ggcgcatttc
     4621 tgggccggta tcgtgctgct cgccctgggc cacgtgctga tctttgccac ggggccgtcc
     4681 ggggagcacc tgcgcccgct ctcagactac tggttcgctg cggtctggtt cggctacatt
     4741 ttcgcgctgg atgccctcgt ctacaggaga gacggcaggt cgctgttcgt caaccaccgc
     4801 aaggtcttcc tgtggatgtt gcccctatcc gcgctcgtat ggctcggctt cgagggtgtg
     4861 aactggttcg tcaagaactg gaactatcta cgcccgttcg acatcccgga gtggtgggcg
     4921 caactctggt ct
//
LOCUS       DASRWZ010000043         6751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_551020, whole genome shotgun sequence.
ACCESSION   DASRWZ010000043 DASRWZ010000000
VERSION     DASRWZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551020"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFH60_00565"
     CDS             complement(<1..551)
                     /locus_tag="VFH60_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET6312299.1"
                     /translation="MQTVNEISPEMIEQFVIAAHGNEEVVREMHAQQPYLVNVTWDKF
                     NETPLQAASHMGNRSIARYLISQGAHPDITTHAMLGDAEQVARCLEADPEQVHARGAH
                     GISLMYHAALSGKPEITELIVSHGGGEGIDDALHAAVSFGHVDMVRWLLENGVQNVNV
                     PNYDKKTPLSVALANGHTEIAALL"
     gene            complement(579..1922)
                     /locus_tag="VFH60_00570"
     CDS             complement(579..1922)
                     /locus_tag="VFH60_00570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6312300.1"
                     /translation="MAHDTGTAEGVMDLGDGLIMRAARTEEAEDLARFHATQQAYPPD
                     TYDETIYHWMLELMSGRHPTFPPSDFLVVEDTVKGQIASSMSLISQVWSYGGVPFKVG
                     QPELVSTHPDYRRKGLVRKQFEVVHRWSAERGELAQTITGIPWYYRQFGYELAIPSGG
                     GRAGYTPHVPPLKEGAPEPYSVRPATEADIPFLSQMLEQMSRRYLITVVKDEQQLRYE
                     IYGRNADTAVRKVLCVIETADAGPVGMLLHDHETQGTRIAAYAYEVKPGVSWVAVTPS
                     VVRYLAATGREYARKKDGDWNTYMFSLGTEHPAYAALLDRLPRVIKPYAWYVRVPDIA
                     AFVRHVAPAIEQRIPGSAVEGHTGEVRLNFYRDGLLLGFEGGKIARAEAWRSGRTEEG
                     DAGFPNLTFLQLLFGFRSLEDLEYAFPDCWVRGDEVRALLNTIFPRRPSSVWANY"
     gene            complement(2314..6276)
                     /locus_tag="VFH60_00575"
     CDS             complement(2314..6276)
                     /locus_tag="VFH60_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET6312301.1"
                     /translation="MTLGSFFQPSFLLLFLRRWGLFILGLLGIAIVLSIGTSTASACP
                     EPCTIVDETTDPPEQLGPGYRFTVSANTEFGEGLMVELNYGATYIRYDCEYLNGSNLP
                     PGTTARYTCSTDQDIPQGTEVNYQYAICDYGTTGTNCHSYTGFVYSFTAPAPPEPPPT
                     SCEGASVGDDDIYWVGLGHNSFDPLYRNPTGPVPTDTLSVNLKFRTCDNDASVARVRI
                     WNDVDNVETVLTMTQAMTATDPLIGDVRYWSVNVSIPTTPTILYYVFRATDGDRTAHY
                     RAKFGNAYSGGSASTWGALGGWGDATGNQAEAEGNSYQLTVYDAGFDVPEWMQQGIVY
                     QIFPDRFRDGDSDNNPIAGRFFYSETGSVVRSGDTDWNTKICDPRNIATPGCPGIFGQ
                     NFYGGDLQGITDKINQGFFDNLGVNVLYLNPIFRSPSNHKYDTADYKVIDPDFGTLAD
                     FQEMVKAARDHGIKIMLDGVFNHTASDSKYFDRYSRYDVTGTLTSPLGQGTNDGSGAC
                     EATTSPFRSWFYFPHTHAAGRDGDPGSGPLALCAPPDPPSAPATTYEAWFGYSSLPKL
                     QANSTQVRQLIWSDGLNSVAPYWTQQGADGWRFDVGADVDQGLPNPFNNDYWEGFRAA
                     VRNPAVTGKSDVVMLGEEWGDTSAWLLGNEWDSTMNYRFRAALLSWLFTSCLDESPGC
                     SDDARGEKFEENDSNEFSSSGPLGYLSPSQLDTRLRTIQEDYPPMAFKAMMNLEGSHD
                     TQRLRFLLKKGNDDSDAAAVQRMKEWWLFSYTYPGAPTLYYGDEMGLSEDGVFAGNWQ
                     DDPYNRVPFAWGDTPGDYTSDTSNLLPHARHMSSLRLSYRALQDGDVQHGLIVSDTAK
                     LYGFGRTGDDMTALVALNRDNAAHTATFSGLNAAPYNLTNGTVLRDGLSAMTYTVASG
                     VVTVTVNSNWGVVLLEEGEIDTPVAPSPSGSRSGGNVTLSWSPVVTDTEGGQELATVY
                     RVYRSTLPAFVPSASNQVGQDVAPPPFGTADGKISFTDPGAGGTEYFYVVRACNAAGN
                     CSDSAPVFVPSGAPTATPTPTQSHTPTQPPTQQPTFTPTSTRTVAATATGVPTECPIQ
                     FQDVPPSNEVSSFYPFVRCLACRGVLGGYPCGGTNPQTGQAEPCGLTGNPYFRPNNLI
                     TRGQIAKVVSNAAGFDEEPQGQTYADVPPSDEPSSFYPFIERLTAHNVMSGYPCDMVP
                     SEPCDGQNRAYFRPGANATRAQLAKIVSNAAGFVNDVEGQTFADVPPPAQPNDPSSFY
                     TFVERLAQRGVMGGYPCGGPGERCDDQNRPYFRPGNPVTRAQAAKIVANTFYPNCQTP
                     "
BASE COUNT         1133 a         2001 c         2245 g         1372 t
ORIGIN      
        1 aggagggcgg ctatctcggt gtgaccgttc gccagggcca cgctgagggg ggtctttttg
       61 tcgtagttgg ggacgttgac gttctgcacg ccgttttcca gcagccagcg taccatgtcg
      121 acgtggccga agctcaccgc cgcgtgcagg gcgtcgtcta tgccttcccc gccgccgtgc
      181 gacactatca actcagtgat ttcgggcttg ccgctcaggg ccgcgtggta catcagcgag
      241 atgccgtgcg cccccctcgc gtggacctgc tccgggtcgg cttcgaggca gcgggccacc
      301 tgctcggcat cgcctagcat ggcgtgggtg gtgatatcgg ggtgcgcgcc ctgcgagatg
      361 aggtagcggg ctatcgacct gttgcccatg tgcgaggcgg cttgcagggg cgtctcgttg
      421 aacttgtccc aggtgacatt gaccaggtac ggctgttgcg cgtgcatctc ccttactacc
      481 tcttcgttgc cgtgggccgc tattacaaat tgctcgatca tttccgggga tatttcgttt
      541 acggtctgca ttacatattc ctttccgcgt tgccgctgct agtagttagc ccacaccgag
      601 gagggccgcc tgggaaagat ggtgttgagc agggcgcgga cctcgtcgcc acgcacccaa
      661 cagtcgggga aggcgtactc caggtcttcc agcgagcgga agccgaaaag tagctgcaaa
      721 aacgtcaggt tggggaagcc cgcgtccccc tcctcggtgc gtcccgacct ccaggcttcg
      781 gcccgcgcta tcttaccccc ctcaaagccg agcagcaggc cgtcgcggta gaagttgagc
      841 ctcacctcgc ccgtgtgccc ctcgaccgcc gagcctggta tgcgctgctc gatagccggg
      901 gcgacgtggc gcacgaaggc agctatatcg ggcacccgca cgtaccaggc ataaggcttg
      961 atcacacggg gcagccggtc gaggagagcg gcgtaggccg ggtgctctgt gcccaggctg
     1021 aacatgtagg tgttccagtc cccatccttc ttccgggcgt actcgcggcc cgtcgccgca
     1081 agatatcgca ccacggaggg cgtgaccgcc acccacgaga cccccggctt tacctcgtag
     1141 gcatacgccg caatgcgcgt gccctgcgtt tcgtggtcgt gcagtaacat gcccaccggc
     1201 ccagcatccg cggtctctat aacacagagc actttacgga ccgctgtgtc tgcgttgcgc
     1261 ccgtatatct cataacgtag ctgttgctcg tccttcacca ccgtaatcag gtagcgccgc
     1321 gacatctgct ccaacatttg cgacaggaag gggatatcgg cttcggtggc cgggcgcacg
     1381 ctatagggtt cgggcgcgcc ttctttgagc gggggtacgt ggggagtgta cccggcgcgt
     1441 ccgccccctg acggtatcgc cagctcgtac ccaaattggc ggtagtacca ggggatgccc
     1501 gtgatggtct gcgctagctc cccgcgctct gcgctccagc ggtgcactac ctcgaactgc
     1561 ttgcggacga gccccttgcg gcggtaatcg gggtgggtgc tcacaagctc cggttgcccc
     1621 accttgaacg gtacgccccc gtagctccat acctgcgaaa tgaggctcat cgaggaggca
     1681 atctgcccct tcaccgtatc ctcaaccacc aggaagtccg atggcggaaa ggtagggtgc
     1741 ctgccgctca ttagctcgag catccagtga tagatcgttt cgtcgtaagt atcgggggga
     1801 tacgcctgct gcgttgcgtg gaagcgggcg aggtcttcgg cctcctccgt cctggccgcc
     1861 cgcatgataa gcccgtcgcc caggtccatg acaccttccg cggtgccggt gtcgtgtgcc
     1921 aatgcgctct ccttcgatgt ttatcttaaa gagcataaca cacgggtgcc agcgatgtat
     1981 cgggctgcgg aacgattgtg acatttgcgt caagatgggc aagcaggacc aaagcccccc
     2041 ttgtcatcct gagcgtagcg aaggatcagg tgggtaggcg agagtcttca accttgagga
     2101 tgatgttggc attaggaaca ttggtgccgt gatagggaga gtcaaccacg ggagactgat
     2161 ccttcgctgc gctcaggatg acagggccta cttcggtcct gtgcctccca tgtggatagt
     2221 gcccctatct cttatcgcag gaatgtaaaa gacgaaggac gatggtggtg agccatcgtc
     2281 cttcgtccta cttagcgtcc ttgccctggg tcgtcaaggg gtctggcagt tgggatagaa
     2341 ggtgttagcc actatcttgg ctgcctgcgc cctggtgacg gggttgcccg gcctgaagta
     2401 aggtcgattc tggtcgtcgc acctctcgcc ggggccgccg cacgggtagc cgcccatcac
     2461 gccgcgctgg gcgagccgct ccacgaacgt gtagaacgat gacgggtcgt tgggttgcgc
     2521 gggtggcggc acgtccgcga aggtctgccc ttctacgtcg ttcacgaagc ccgccgcgtt
     2581 ggacactatc ttcgcaagct gggcgcgtgt ggcgttagcg ccggggcgga agtatgccct
     2641 gttctgccca tcgcacggct cagatggtac catgtcgcac gggtagccgc tcatcacgtt
     2701 gtgcgcggtg agccgctcga tgaacgggta gaaggagctt ggttcgtcgg acggtgggac
     2761 gtcggcgtac gtctgcccct gcggctcttc atcgaagccc gccgcgttgg acactacctt
     2821 ggctatctgc cccctggtta tgaggttgtt ggggcggaag taagggttgc cggtcagccc
     2881 gcaaggctct gcctgcccgg tctgggggtt ggtgccgcca cacggatagc cgcccagcac
     2941 tcctctacag gcgaggcagc gcacgaacgg gtagaacgag ctcacctcgt tggagggtgg
     3001 cacgtcctgg aactggatag ggcactctgt gggcacgcct gtagctgtgg ccgcgaccgt
     3061 gcgcgtggac gtaggtgtaa acgtgggctg ctgggtgggc ggctgggtgg gcgtgtgcga
     3121 ctgggttggg gtgggtgtag ctgtgggggc acccgagggt acgaataccg gggcgctgtc
     3181 ggagcagtta cccgccgcgt tgcaggcccg caccacgtag aagtactcgg tgccgcccgc
     3241 gccggggtcg gtgaagctga tcttgccatc ggcggtgccg aagggcggcg gggcgacatc
     3301 ctggccgacc tggttgcttg cactcggcac gaacgcgggg agcgtgctgc gatagacccg
     3361 gtaaaccgtg gctagctctt gcccaccctc cgtatcggtt acgaccgggg accaactcaa
     3421 cgtcacgttg ccgccggagc ggctgccgct ggggctgggg gcaaccggtg tgtcgatctc
     3481 gccttcttcg agcagcacca cgccccagtt gctgttcaca gtgacggtaa ccacgccact
     3541 cgccaccgtg taggtcatgg cgctcagacc gtcgcggagc acggtgccgt tagtcaggtt
     3601 gtagggagcg gcgttgaggc cgctgaaggt ggctgtgtgg gccgcgttgt cgcggttgag
     3661 ggcgaccagg gcagtcatat cgtcgccggt gcgcccgaag ccgtacagct tggcggtatc
     3721 gctcacgatg aggccgtgct gcacatcgcc gtcttgcagg gcgcggtagc tcaggcgcag
     3781 gctcgacata tgccgggcgt gcggcagcag gttgctcgtg tcggacgtgt agtcgccggg
     3841 ggtatcgccc caggcaaaag gcactcggtt gtacgggtcg tcctgccagt tacccgcgaa
     3901 cacgccgtcc tcgctgagtc ccatctcgtc gccgtagtag agggtgggcg cgccggggta
     3961 ggtgtaggag aagagccacc actccttcat gcgctgcacg gccgccgcgt cgctgtcgtc
     4021 gttgcccttc tttagcagga agcgcaggcg ctgcgtatcg tgcgagcctt ccaggttcat
     4081 catcgccttg aaggccatcg gcgggtagtc ttcctgtatc gtgcgcagcc gtgtgtcgag
     4141 ctgcgagggg ctgaggtagc cgagagggcc gctggagcta aactcgttcg agtcgttctc
     4201 ctcgaacttc tcgcccctgg cgtcgtccga acagccgggg gattcgtcaa ggcagcttgt
     4261 aaagagccag ctaagcaggg cggcgcggaa gcggtagttc atcgtgctgt cccactcgtt
     4321 gccgagtagc caggccgagg tatcgcccca ctcctcgccc agcatgacca cgtcggactt
     4381 gccggtcacg gctgggttac gtacggcggc gcggaagccc tcccagtagt cgttattgaa
     4441 cggattcggg aggccctggt ccacgtccgc gcccacgtcg aagcgccagc cgtccgcgcc
     4501 ctgctgcgtc cagtacgggg cgaccgaatt gaggccgtca ctccagatga gctggcggac
     4561 ctgtgtcgag ttggcctgga gcttgggcag ggacgaataa ccgaaccacg cttcgtaggt
     4621 ggtggcgggc gcgctcggcg ggtcgggagg ggcgcacagg gccagcggtc ccgagcctgg
     4681 atcgccgtct cggccggcgg catgggtgtg cgggaagtag aaccacgagc ggaaaggcga
     4741 agtcgtagct tcgcacgcgc cactaccgtc gttcgtaccc tgtccgagag ggctggtgag
     4801 ggtgccggtt acatcgtagc ggctgtagcg gtcgaagtac ttgctatcgg aggcggtgtg
     4861 gttgaacacg ccgtcgagca tgatcttgat gccgtggtcg cgggccgcct tcaccatctc
     4921 ctggaagtcg gccaacgtgc cgaagtcggg gtctatcacc ttgtagtcgg cggtatcgta
     4981 cttgtggttc gacggggacc ggaagatcgg gttgaggtag agcacgttca cgccgaggtt
     5041 gtcgaagaag ccctggttga tcttatcggt gatgccttgc aggtcgccgc cgtagaagtt
     5101 ctggccgaag atgccgggac agcccggcgt ggcgatgttg cgagggtcgc agatcttggt
     5161 gttccagtcc gtgtcgccgg aacgcactac cgacccggtc tcgctgtaga agaagcggcc
     5221 cgcgataggg ttgttgtccg agtcgccgtc gcggaagcgg tcggggaata tctggtagac
     5281 gatgccctgc tgcatccact caggcacgtc aaaacccgcg tcgtacacgg tcaactggta
     5341 ggagttaccc tcggcctcgg cctggttgcc cgtagcatcg ccccagccgc cgagtgcgcc
     5401 ccaggtgctc gcgctgccgc cggaataggc gttaccgaac ttggccctgt agtgtgcggt
     5461 cctgtcgccg tcggtggcgc ggaagacgta gtagaggatg gttggggtcg tggggatgct
     5521 gacgttcacc gaccagtacc tcacgtcgcc gataagaggg tcggtggcgg tcatcgcctg
     5581 cgtcatcgtg agcacggtct ccacgttgtc cacgtcgttc cagattctca ccctggctac
     5641 ggaagcgtcg ttgtcacagg tgcggaattt gaggttgacg gatagcgtat ccgtgggcac
     5701 ggggccggtg gggttgcggt acaaggggtc gaacgagtta tgccccaggc cgacccagta
     5761 gatatcatcg tcgcccaccg aagcgccctc gcacgaagta gggggaggct ccgggggtgc
     5821 gggggccgtg aaagagtaga cgaagcccgt atacgagtgg cagttagtgc cggtggtgcc
     5881 gtaatcgcat atggcgtatt gatagtttac ctcggtaccc tgtgggatgt cctggtccgt
     5941 gctacaggtg tagcgcgcgg tagtgccggg gggcaagttg ctgccgttga ggtactcgca
     6001 atcgtacctg atgtaggtag caccgtagtt aagctctacc atgaggccct caccgaactc
     6061 ggtgttggcg gataccgtga agcggtagcc cggaccaagc tgctccggtg ggtccgttgt
     6121 ttcgtctacg atggtgcatg gctcggggca ggccgaagcc gtagaggtgc cgatgctcag
     6181 cacaatagcg attcccagca ggccgaggat gaatagtccc cacctgcgca aaaagagcag
     6241 caaaaacgac ggctgaaaaa agctccctag agtcatcagt tcctcctcga cttccttgca
     6301 ttattgccgt acttgacgta acgaccggcg cttgtgacat tacccgcggc agccaggtat
     6361 gtagtgaatc acgcgggcca caggcccagg gcagcgcgag agtcctatcc gcaagattct
     6421 tgccaggagt acgcggttcg cgctatgatg accccgcttt agccctgcgg ggacgccctg
     6481 ccaatgaatt ggcgggctta ccagtgacga agtctcctac ggagactacc ttcttgttgc
     6541 ttagtccccg taggggactt tgccccttgt aagcccgtgg ctttatccac agggcgttgt
     6601 tgccgcgcct ctgccgagaa tgcctacatc ctgccctaag ccaatccacc cttcgccata
     6661 gaccaatctg gtggtatact tgagccagca gaaggcgaca cggccctgta gcccctgtaa
     6721 atgtctgctt tgcccggtta tcttgggggt a
//
LOCUS       DASRWZ010000044         4062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_688105, whole genome shotgun sequence.
ACCESSION   DASRWZ010000044 DASRWZ010000000
VERSION     DASRWZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688105"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFH60_00580"
     CDS             <1..590
                     /locus_tag="VFH60_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6312302.1"
                     /translation="RGVISGYSSSPPCDTASHVPCFLPNRNLTRGQGAKIVALAAGVP
                     AAPAGQRFADVAPGSPFYNEINALAALGHMGGYPCGSAGEPCTGGNLPYFRPGSSITR
                     GQLSKIVSNTAGYNEAHSTQNFEDVPTNGAFYIWIERLASRGIINGYTCGGAGEPCNP
                     PANRSYFRPNAEITRAQTAKIVYGVWPTQTQPAKR"
     gene            740..1852
                     /locus_tag="VFH60_00585"
     CDS             740..1852
                     /locus_tag="VFH60_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312303.1"
                     /translation="MLRSRQYLAPSIFALALCIGLYLLPSTQGAATPLAQRPTPVLPT
                     PTPAARPIFLTDGVADPVERGVSWFAPTGHTLRGSFLGYWHEQGGLPQFGYPISEEFT
                     EPIGPKGAPIMVQYFERARFEHHPENAGTPHEVLLGALGREFHPPAPPASPLPSPDIF
                     PPSRVYFSETGHNVGGAFLKYWNEHGGLAVHGYPISEEFEEKSPTDGEMYRVQYFERS
                     RFELHPENAGSPYEVLLGQLGTQLSQKKGYPYGWYPLYGHAADFSWVAGIPQWQEVRF
                     DCNIAYAGYCDCITLRYEQAGMRLQLDTIRMPLPTLQRLRDATQQLDQAPRPQVIFGR
                     FSLEEEKLVRCTPPVTARGYLPQHTQDNPATAGVGR"
     gene            1923..3920
                     /gene="acs"
                     /locus_tag="VFH60_00590"
     CDS             1923..3920
                     /gene="acs"
                     /locus_tag="VFH60_00590"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET6312304.1"
                     /translation="MHPTKEPSGDGAGTIESLLNEERLYPPSPEFVAQANVSDPEVYA
                     RAEADPIAFWREQAGVVEWFKQPTDTLQWNPPFAKWFQDGELNACYNAVDRHLPHRAN
                     KPAIIWEGEPGDTRTLTYSELYREVNRAAAMLRNLGVGKGDRVAIYMPMIVEAVVAML
                     ACARLGAPHTVVFGGFSPEALRDRIIDADTKLVITADGGYRRGGIVPLKANTDAALDA
                     PDLPVEKVLVVQRLGEGTQTNMQAGRDIWWHEALRDVADDAIVEPERVGAEHPLFILY
                     TSGTTGKPKGVLHTTGGYMVGTTLTSNWVFDLKEDDVYWCTADIGWVTGHSYLVYGPL
                     SNGVTAVMYEGTPDHPDKDRWWQLIEKYRVTILYTAPTAIRTFMRWGTEYPERCDLSS
                     LRLLGTVGEPINPEAWIWYHKHIGNEQRPIVDTWWQTETGAIMITPLPGLVATKPGSA
                     TRPFPGIVAQIVDEWGNPVEKGVSGGLLVIKQPWPSMLRTIWGDDERYKVQYWGKFNR
                     QYDVYFTGDGSHQDEDGYFWLMGRVDDVLNVAGHRLGTMELESALVSHPAVAEAAVVG
                     VTHELKGQGVVAFVTPRSGHQGTDQLAEELRRHVAEKIGPIARPDRIYFTAELPKTRS
                     AKIMRRLLRDIAEGRVLGDTTTLSDPTVMASIKQQYESTEG"
BASE COUNT          801 a         1342 c         1245 g          674 t
ORIGIN      
        1 accggggcgt gatttcaggc tacagcagct cgccgccttg cgacacggcc agccacgtgc
       61 cctgcttcct acctaaccgc aacctcacgc gcgggcaagg ggctaagata gtggcgctgg
      121 cggcgggggt ccctgccgcg ccagcggggc agcgattcgc ggacgtagcg ccgggcagcc
      181 ccttctataa cgaaatcaac gccctggctg ccctgggcca catgggaggc tacccctgcg
      241 gcagcgcggg cgagccgtgc accgggggca acctgcccta cttcaggccc ggtagcagca
      301 taaccagggg ccagctatcc aaaatcgtct ccaacacggc gggctacaac gaagcgcact
      361 ctactcagaa cttcgaggac gtgcccacca acggtgcctt ctacatctgg atcgagcgcc
      421 tggcgagtcg gggcatcata aacggctaca cgtgcggcgg agccggggag ccgtgcaacc
      481 cacccgccaa ccgctcctac ttccggccca acgccgagat cacgcgggcg cagacggcca
      541 agatcgtgta cggagtgtgg cccacacaga cgcaaccggc taagaggtag acctattcgc
      601 gggaccctgg ctgagcaaag cggcctcctg agcatacctg gaggccgctt ccgcatttgc
      661 acctgctgct ctataatgtg agcgtcatcg ccctgtgtag aatcgccggt cgctcctcta
      721 tccggcaaag gaggctgtca tgctacgaag caggcagtat ctcgcccctt ccatatttgc
      781 gcttgccctc tgcatcgggc tttacctgct gccgagcact caaggagccg ctactcccct
      841 cgcgcagagg cccacacccg tactacccac acccacaccc gcagcccgcc cgatattcct
      901 taccgatggc gtggccgacc cggtggagcg tggcgtctcc tggtttgctc ctaccggaca
      961 taccctgcgt ggtagcttcc tgggctactg gcacgagcag ggcggccttc cgcagttcgg
     1021 ctaccccata tcggaggagt tcacagagcc aattgggccg aagggcgcgc ccatcatggt
     1081 gcagtatttc gagcgcgccc gcttcgagca ccaccctgag aacgcgggca ctccccacga
     1141 ggtgctgctt ggcgcgcttg gccgcgagtt ccacccgcca gccccgcctg cttcgcccct
     1201 gccctcgccc gatatattcc ctccgagcag ggtctacttt agcgagacgg ggcacaacgt
     1261 gggaggtgct tttctcaagt actggaacga acatggcggg ttagcggtac acggctaccc
     1321 tatatcggag gagttcgagg agaaaagccc caccgacggc gagatgtatc gcgtgcagta
     1381 cttcgagcgg tcgcgcttcg agttgcaccc ggagaacgcc ggtagccctt acgaggtgct
     1441 cctggggcag cttggcacgc agcttagcca gaagaagggc tatccctatg ggtggtaccc
     1501 gttgtacggg catgctgccg acttctcctg ggtggcggga attcctcaat ggcaagaggt
     1561 gcgatttgat tgcaatatag cttatgccgg ctactgtgac tgtataactc tacgatatga
     1621 gcaagccggt atgcgcctcc aactggatac tatcaggatg ccgcttccca ctttgcagcg
     1681 cttgcgagat gctacccagc aactggacca ggctccccga cctcaagtga tctttgggag
     1741 attttcactc gaggaggaaa agctggtgcg ttgcacacca cccgtcaccg ccaggggtta
     1801 tttgccgcag cacacgcagg acaaccccgc tacagcagga gtgggacgct agctctttgc
     1861 tattcccacc gcttgcgata tactgggggc actaatcccc atccctagag cggaggtcaa
     1921 cgatgcatcc gacgaaggag ccttccggcg acggcgcagg cacgattgag agccttttga
     1981 acgaggagcg cctctacccg ccctcgcccg aattcgtggc ccaggccaac gtctccgacc
     2041 ccgaagtgta tgcccgcgca gaggcggacc ccatcgcctt ctggcgcgag caggcaggcg
     2101 tggtcgagtg gttcaagcag cccaccgaca ccctccagtg gaacccgccc tttgcgaagt
     2161 ggttccagga cggcgagctg aacgcctgct acaacgccgt ggacaggcat ttgccccacc
     2221 gcgccaacaa gcccgctatt atctgggaag gcgagccggg cgacacccgc accctcacct
     2281 acagcgagct ataccgtgag gtaaaccgcg ccgccgccat gctccgcaac ctgggcgtgg
     2341 gcaagggcga ccgcgtggcg atctacatgc cgatgatagt ggaggccgtg gtggcgatgc
     2401 tggcctgcgc ccgcctgggt gcgccccaca ctgtggtgtt cggcggcttc tccccggagg
     2461 ccctgcgcga ccgtatcatc gacgccgaca ccaagctggt aatcaccgct gatggcggct
     2521 accggcgcgg cggcatcgtg cccctcaagg ccaacaccga cgcagccctc gacgctcccg
     2581 acctgcccgt ggagaaggtg ctcgtcgttc aacgcctggg cgagggcacc cagacgaaca
     2641 tgcaagcggg ccgcgacatc tggtggcacg aagccttgcg ggacgtggcg gacgacgcaa
     2701 ttgtggagcc ggagcgcgtg ggggccgagc acccgctctt catcctctac acgtcgggca
     2761 ccaccggcaa gcccaagggc gtgcttcaca ccaccggcgg ctacatggtg ggcaccacgc
     2821 ttacctccaa ctgggtgttc gacctgaagg aggacgacgt ttactggtgc accgccgaca
     2881 tcggctgggt gacggggcac tcttacctcg tgtatggccc cctctccaac ggtgtcacgg
     2941 cagtcatgta tgagggcacc cccgaccacc ccgacaagga ccgctggtgg cagttgatcg
     3001 agaagtacag ggtgaccatc ctgtacaccg cgcccaccgc catacgcacc tttatgcgct
     3061 ggggcaccga gtacccggag cggtgcgacc tgtcgtccct ccgtctgctg ggcacggtgg
     3121 gcgagcctat caacccggag gcgtggatct ggtaccacaa gcacatcggc aatgagcaac
     3181 ggcccatcgt ggatacctgg tggcagaccg agacgggggc gatcatgatt acaccgctgc
     3241 ccggcctggt cgcgaccaag cccggctcgg ccacccgccc cttccccggc atcgtcgcgc
     3301 agatagtgga cgagtggggc aaccccgtag aaaagggggt gtcgggcggc ctgctggtga
     3361 taaagcaacc ctggccgtcg atgttgcgca cgatatgggg cgacgacgag cggtacaagg
     3421 tgcagtactg gggcaagttc aaccgccagt acgacgttta cttcaccggc gacggctcgc
     3481 accaggacga ggacggatac ttctggctga tgggccgtgt ggacgacgtg ctcaacgtgg
     3541 cggggcaccg cctgggcacg atggagcttg agagcgccct ggtgagccac cccgccgtgg
     3601 ccgaggccgc agtggtgggg gtgacgcacg agctgaaggg gcagggcgtg gtggccttcg
     3661 tcacccctcg ctccgggcac cagggcaccg accagttggc cgaggagcta aggcggcacg
     3721 tggccgagaa gatcggcccc atcgcccgcc ccgaccgcat ctacttcacc gccgagctac
     3781 ccaagacccg ctcagccaag atcatgcgcc gcctcctgcg cgacatagcc gagggccgcg
     3841 tcctgggcga caccaccacc ctctccgacc ccaccgtcat ggcctccatc aaacaacaat
     3901 acgagtccac cgaggggtaa gagacgagta tgccaacgga taaagagacg aaaacgcaaa
     3961 gacgcaaagg acgcaaagaa cgcgcaaaga gtagttaaag gttagagggt acaaaaagga
     4021 ccagagtgcg aaatgacgta ctctggtcct ttttgttagg ac
//
LOCUS       DASRWZ010000045         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1099299, whole genome shotgun sequence.
ACCESSION   DASRWZ010000045 DASRWZ010000000
VERSION     DASRWZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099299"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1421
                     /locus_tag="VFH60_00595"
     CDS             225..1421
                     /locus_tag="VFH60_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6312305.1"
                     /translation="MNDLTLTNVRLIDGTGSQPRDNVVVTVRGGRIESVRDGGEQAGD
                     GGDVIDLGGRTLLPGLINAHCHVTMNAGPDPFAYTNNTSLPTLVLHSVRRCEQMLRAG
                     ITTARDLGGYEYIELALRDAFASGDWPGPRLLCAGKVLTMTGGHGWPIGMEVDGPDEV
                     RKGARENLKKGADCIKIMATGGVLTPGVEPGAQQLGEEEIRAGVEEAHKVGKLAATHA
                     QGTEGIKAALRAGIDTVEHGIFLDEEAIQMMVERGVVFVPTLAAPYQIVEAGEERGIP
                     AYALEKSRRVLEAHTRSFEWALTAGVTIAAGNDGGTPFNPAHDLVTELRLMTEHGLNT
                     LGAIRVATLGSAKALGLSEEIGTVQAGKWADLLVLEEGADPIADITALGRVWLVVKQG
                     KVVVRS"
     gene            complement(1443..2555)
                     /locus_tag="VFH60_00600"
     CDS             complement(1443..2555)
                     /locus_tag="VFH60_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET6312306.1"
                     /translation="MSSGTVILLTGMLVAVACAMLGTFLVLRKMAMMADAISHAILPG
                     LVLSYFLANGPNLLAGFFGAALAAVITVSLVEALQKTRRVGGESAIGLVFPAMFALGT
                     FFVSRFFSDVHLDTDAVLYGNIEFAAQDVLIVGDTSFGPQSLWVMAFLCLMNGLFISL
                     FYKELKLATFDPGLAGTLGFSPFLLHYALMSVTSVTAVGAFTAVGAVLVVALMIVPAT
                     TAYLLTDKLPVMIGLSIGIGAASAVSGYYLAVVLDSSVAGAMASMTGVFFGLALLFSP
                     GQGIVARVRRLAEQRAQFAAETLVVHLFNHENTPQAAAESSVGHLHDELLWSAELSQR
                     AIRRATSLGLIRREADQLSLTPEGRDIARDLASEAA"
BASE COUNT          521 a          783 c          903 g          430 t
ORIGIN      
        1 ccaagaagaa taattgttat tcaatcaaga ggaccggggt acgtaacatc gcccctggtc
       61 ctttttatac cctctaaacc gctattatct tggcgtcctt ggcgtcttgg cggttccgtc
      121 ttcgaatcac cagggagcgg agtttgctct agcaaggggg ttgggcagac agggacacgg
      181 catacttagg cttgactcat ctcacgacaa ggggaacatc cgcgatgaac gatcttacac
      241 ttactaacgt gcggctgatt gacggtacgg ggtcccagcc gagggacaac gtagtcgtca
      301 cggtgcgcgg agggcgcata gagagcgtgc gggacggtgg ggagcaggcg ggggatggag
      361 gagatgtcat cgaccttggg gggcgcacgc tgctgcccgg cctcataaac gcccactgcc
      421 acgtcacgat gaacgccgga cccgacccgt tcgcctatac taacaacacg agcctcccca
      481 ccctggtgct gcatagcgtc aggcggtgcg agcagatgct gcgggcgggc attactactg
      541 cacgcgacct ggggggctac gagtacatcg agctggccct gcgcgatgct ttcgcctcgg
      601 gcgactggcc ggggccgcgt ctgctgtgcg cgggcaaggt gctcactatg acgggtgggc
      661 atggctggcc tatcggcatg gaggtagacg ggcctgacga ggtacgcaag ggtgcccgcg
      721 agaacctcaa gaaaggcgcg gactgcatca agataatggc taccggcggc gtgctcacac
      781 cgggggtgga gccgggcgcg cagcagttgg gtgaggagga gatacgggcc ggggtggagg
      841 aggcccacaa ggtcggtaag ctggcggcca cgcacgcgca gggcaccgaa ggcatcaagg
      901 ccgcgttgcg ggcgggcatc gacaccgtag agcacggcat cttcctggac gaagaggcga
      961 tacagatgat ggtggagcgc ggggtggtgt tcgtgcccac gctggcggcc ccttaccaga
     1021 tagtggaggc gggggaggag cgcggcattc ccgcctacgc gcttgagaag tcgcgccggg
     1081 tgctggaggc ccacacccgg tcgttcgagt gggcgctcac cgcgggggtg actatcgcgg
     1141 cgggcaacga cggcggcacg cctttcaacc ccgcgcacga cctcgtaacc gagctgcgcc
     1201 tgatgaccga gcacggcctc aacacgctgg gcgcgatacg ggtcgccacg ctcggttcgg
     1261 ccaaggcgct ggggctatcg gaggagattg gcacggtgca ggccggtaag tgggccgacc
     1321 tgcttgtgct ggaggagggt gccgacccca tcgcggacat aacggccctg gggcgggtgt
     1381 ggctggtggt caagcagggg aaggtggttg tgcggagtta gggaagcaca gccaaaccgc
     1441 ttctaagccg cttccgaagc cagatcacgc gctatgtcgc ggccctctgg ggtgaggctc
     1501 aactggtcgg cttcgcgcct gatgaggccc agggaagtgg cgcgcctgat cgcacgttga
     1561 gacagctcgg cggaccagag gagctcgtcg tgcaggtgcc ccaccgagct ttcggcggcg
     1621 gcctgcgggg tgttctcgtg gttgaacagg tgcactacca gcgtttcggc ggcgaactgc
     1681 gcccgctgct cggccagcct gcgcaccctc gccacgatgc cctggcccgg agagaacagc
     1741 agcgccagcc cgaagaacac gcccgtcatc gaggccatag ccccggccac cgaggagtcc
     1801 agcaccaccg ccaggtagta gccgctcacg gcggaggccg caccgatgcc gatggacagg
     1861 ccgatcatga caggtagctt gtcggtcagc aggtacgcgg tggtggcggg cactatcatc
     1921 agggccacca ccagcaccgc gcccaccgcc gtgaacgcgc ccaccgccgt taccgaggtg
     1981 acgctcatca gggcgtagtg caggaggaac ggggagaagc cgagcgtgcc cgccaggccg
     2041 ggatcgaagg tcgccagctt tagctctttg tagaacaggc tgatgaagag gccgttcatc
     2101 agacagagga aggccatgac ccagagggac tgagggccaa aactggtgtc accgacgatg
     2161 agcacgtcct gcgcggcgaa ctcgatgttg ccgtaaagca ccgcgtcggt gtcgaggtgc
     2221 acgtcggaga agaagcggga cacgaagaag gtgcccagcg cgaacatggc cgggaacacc
     2281 aggcctatgg ccgactcgcc ccccacccgc cgggtctttt gcagcgcctc caccaggctg
     2341 accgtgatta ccgccgcgag cgccgccccg aagaagcccg ccagcaggtt aggtccattc
     2401 gccaggaagt acgacagcac caggccaggc aggatggcgt ggctgatagc gtcggccatc
     2461 atagccatct tgcgcagcac caggaacgtg cccagcatgg cgcacgccac ggcgaccaac
     2521 atgcccgtta gtagtatgac tgtgcctgaa ctcattgtgc tcccgcaatt cttaatgttt
     2581 aattcttaat tcttaattgt cggtttcttc aattatcttt gataaggcca taaagaa
//
LOCUS       DASRWZ010000046         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_71919,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000046 DASRWZ010000000
VERSION     DASRWZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71919"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            769..1392
                     /locus_tag="VFH60_00605"
     CDS             769..1392
                     /locus_tag="VFH60_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312307.1"
                     /translation="MKCTPEEYAFHQRVLARDPVAFSQLAEWLYASLVRDTGVRARGR
                     AGKAVDMMLIEESVGEALLDYNDAPERYDPHQATLHSYLVMVAYRDFQNALSKELRHV
                     HRQTTMGADGQAEMDVPDARGELEDLLNRISAEEWWSLVEGTFSDATDRQLVIMLANG
                     VRSTESYARVLGITGLGGEEQAREVKKAKDRLLKRLKRLGESHDERT"
     gene            1379..1822
                     /locus_tag="VFH60_00610"
     CDS             1379..1822
                     /locus_tag="VFH60_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312308.1"
                     /translation="MSEHDTQPSLGRLASLAAGRPSLMSSLMQLYREQEGMNDQQLAA
                     FLECEAEALPRLALCRRPRQAPDFRGDIEAIAAHTGANPTRLAGLVRAAEGLQALREG
                     HEAGPTTPITNTTTSMLMAARDYNESAGEEDDSSPGDEAGEDDSH"
     gene            1809..2561
                     /locus_tag="VFH60_00615"
     CDS             1809..2561
                     /locus_tag="VFH60_00615"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HET6312309.1"
                     /translation="MIATDSAIENAAREFWTLAGGVPGYPCDLESSIPYALPLQVRAV
                     PGLRVSHANGYARDRGLPYRFSGRDRRLCGCLLAYFDVGIIFLDSGIAEDERRFTLAH
                     ELAHFLLDYRAPRLRCIEALGEGIRAVLDGLRPPTVEERVHAVLAGTRLGVVGHLMER
                     PAAGLPSSTVLGIESRADRLALELLAPAWFVMEKAEAWRGLSHRERVSALAVLLVREH
                     GLPVGVAGKYASGLLRRNGNGPGFRDWLSGED"
BASE COUNT          492 a          789 c          848 g          476 t
ORIGIN      
        1 gttgaggacg tgctagccgc gcgttactta gcggagggtg tcgctcccta cgggacggtt
       61 ataacgacag gcagcaatat ccggaaggta gcaccccggc ccgccttgcg gcctcagacg
      121 ggaacaccag acatggcaca atgccagcga gagaggcgct gaagaggtta ttagattgta
      181 aagtacgagc atacgacagc cgcctgttct tccgatagcg ttgcgtgacc gaagcggagg
      241 cccggatcga cacgtaccca gcaagagacg gaaccgccag gacgccaaga acaccaagaa
      301 cgcgccaaga ctaagtaggt gctctaagta caaagaggac cagggtacaa ccttctgtac
      361 tccggtcctc ctgcttggaa cagcacaaca atctctgcgt aacctctgcg ttccttcggc
      421 tgcgctcagg gcaggctctc tgcgtctctg cggtggaccg ttcttgttct tggcgctctt
      481 ggcgtcttgg cggttccgtc tcttgccatg taactatccc cgcttttgta agctagtggc
      541 gttagcaggg cacgatttag ctgtacattg cgattgctaa gaacacttgc atgtgatata
      601 gtaaggccca agtacgcgtg ctagtgacat cactgttact tcgttcgtga ctactccccg
      661 gtcgccgcac cgccggttac ctgtcgcgta ttatttcgcg ctccaccctc tatcgcgtcg
      721 aacttactcc atttccctgg aatagattat gaaaggtatt tccgcaacgt gaagtgcacg
      781 ccggaggagt acgccttcca ccagcgggtg ctagcgcgag acccggttgc tttctctcaa
      841 ctggcagaat ggttgtacgc aagcctggtg cgggataccg gggtgcgcgc ccgcgggcgc
      901 gccggtaagg ctgtggatat gatgctcata gaggaatctg taggcgaggc gctgctcgat
      961 tacaacgacg cacccgaacg ctacgacccc caccaggcta ccttgcatag ctacctggtc
     1021 atggtggctt accgcgactt ccagaacgcg ttgagcaagg agctccggca cgtccaccgc
     1081 cagaccacga tgggggccga tgggcaagcc gagatggacg tgcccgacgc caggggcgag
     1141 ctggaggacc tgctcaaccg catctcggcg gaggaatggt ggtcgctggt ggaaggcacg
     1201 ttcagcgatg ccaccgaccg gcagttggtg ataatgctgg cgaacggggt gaggtccacc
     1261 gagagttacg cgagagtgtt aggaataacg gggttagggg gcgaggagca ggcgcgggag
     1321 gtcaagaagg ccaaggaccg cctactcaag cgcctgaaaa ggctggggga gtcgcacgat
     1381 gagcgaacat gacacgcagc cgagcctggg ccgcctcgca agcctggccg cgggccgccc
     1441 cagccttatg tcgagcctga tgcagcttta cagggagcaa gaaggcatga acgaccaaca
     1501 gttggcggct ttcctcgaat gcgaggccga agccctgccc cgcctggcct tgtgtcgccg
     1561 cccgcgccag gcacccgatt tcaggggcga cattgaggcc atcgcggccc acacgggcgc
     1621 taaccccacc cgcctggctg ggctggtgcg cgccgccgag ggcctgcaag ccctcagaga
     1681 agggcacgag gccggcccca ctaccccaat tactaatacc accacttcca tgctcatggc
     1741 cgcgcgagac tacaatgaat ctgccggcga ggaagatgac tcgtcccccg gcgacgaggc
     1801 cggagaggat gatagccact gattccgcaa tagaaaacgc cgctcgcgag ttctggacgc
     1861 tggcgggggg cgtgccgggc tacccgtgcg acctcgaaag cagcatcccc tatgcgcttc
     1921 ccctgcaggt aagggcggtg cccggcctcc gggtcagcca cgcaaacggt tatgcccgcg
     1981 acagagggtt gccctaccgc ttttcgggca gagaccggcg tttgtgcggg tgcctgctcg
     2041 cctattttga tgtcggtatc atcttcctgg actccggtat tgccgaggac gagcggcgtt
     2101 ttaccctggc ccacgagctt gcgcacttct tactggatta ccgggccccg cgcctgcgct
     2161 gcatcgaagc cctgggcgaa gggatacgcg cggtgctgga tggcctgcgc ccgcccacgg
     2221 tggaggagcg ggtacacgcc gtattggcgg gcacccggct cggcgtcgtg gggcatttga
     2281 tggagcgtcc cgccgccggg ctgccctcct ccaccgtgct cggcatcgag agccgggccg
     2341 accgcctggc gcttgagctg ctcgccccgg cctggtttgt aatggagaag gccgaggcgt
     2401 ggcgcggcct gtcccaccgg gagcgcgtga gcgcgctggc cgttctgctg gtgcgggagc
     2461 atggcctccc ggtgggtgta gccgggaagt acgcgagcgg actgctgcgc cgtaacggca
     2521 acggacccgg ctttcgggac tggctttccg gagaggacta gcacgcggag gttgtgaggt
     2581 ggcctgaggg ggtgccctgc gatag
//
LOCUS       DASRWZ010000047         5685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_3322,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000047 DASRWZ010000000
VERSION     DASRWZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3322"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="VFH60_00620"
     CDS             <1..1259
                     /locus_tag="VFH60_00620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6312310.1"
                     /translation="YFIGSYSPDLMGNVGHEQAILALQRRGHEVEVLTQINEPGVPRY
                     SRTAYAGVPTYRVNLATFSGRVSGLLRRAAGRFLQYEHTFTLLAALRRHLKRHRYDLL
                     HVEGVYPFGAAAALGAGKTPYLANAQGADVIELPENDYGYRRFRLPRAAVGLALRRAA
                     LVRVNSPFMIGYLVGERLAERERIVMVPRVLEDSAFPPVDAPLHEFRRESRARLSERY
                     GIGLPNPVVMSLSRLHPFKGLEYLVDAMPLVAAEMRAIGKPVPWFILCGPSRSTEHYG
                     DYREFLQKRAAELGVAQHVIFTGQVAHPDVRRHLAGADIVTSPSVVEALHRVALEAAA
                     VGTPTVVSETTGAASYLAPHDACMAVAPRSPQAIADAIIRLLSEANLYERVRDNGLKV
                     AEGLRVEPVADQLEAAWLRAASAGKG"
     gene            complement(1256..3322)
                     /locus_tag="VFH60_00625"
     CDS             complement(1256..3322)
                     /locus_tag="VFH60_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312311.1"
                     /translation="MAFSYPLVLHLSDSVVLSRGSDAWLHLWDLWWVDKAVVDLQQNP
                     YQTTYLYHPTGLNLYYHSLDLLNALVSIPFQRLFNLTAAFNLLTLANLTLNGLAAYWL
                     CRERTGSVEAGLVGGALFASTPLLGTSLDFGQLDEVTVWWVPLYILALWRALDSPGLV
                     WKPGGGRRAAVCAGLCLVGASLATWYFTAGLVVFTAFFVPVYLLGQEPRGVRQYGARL
                     WNAALKVGVGVALFLVLLSPLLWAMIGERLSGATYMLPAPRTTIFNSVDVLALFAPPR
                     LQLPDLNAHNSTVALGYVAIVLALLGLAAPRKLTLPLGVAFIALLVMSLGPVLKIEGS
                     ETGIPMPYALLNNVPFIGASRQPLRFLATAGMVLSLMSAYGFAWLAGRTRGTGLRVGV
                     TAGALLLVALELFGLPRQLVAPQEGGAVAFLRSNTQPGAVIELPYEEYSALSLLHQTA
                     HERPIVGGYTSRHFPYPFADAAPGLAQLVSADPSPLVGEDVLAPPASETASESLHYYD
                     IRYVVVHKADLATGRYGALEEVLEALFGGQSPAYEDEEVAIYEAAEGQAAPGATLPLV
                     GLGSGWHAVEPDPTHRWTGRNVTDGNALVWIGIPPTAEGSYRLQMTAYSYEKPRTVTV
                     ELDGRAISEQSLTPAFSDLDIDLGRLQEGNHILLLRVHEPPENPPGDERKLAIGTTHL
                     AVVRQP"
     gene            complement(3390..3977)
                     /gene="hpt"
                     /locus_tag="VFH60_00630"
     CDS             complement(3390..3977)
                     /gene="hpt"
                     /locus_tag="VFH60_00630"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET6312312.1"
                     /translation="MSGDIHHILVTEEDLKSAIADLGRTLTREYAGKDLLLVGVLRGA
                     IMFIVDLARAIDLPLTIDFMAVASYGASTETSGIVRILKDLDSSIEGKHVLVVEDIID
                     SGLTLNYILDTLRTRNPASVRVAALLNKPARRRVDVGVDYVCFDIPDEFVVGYGLDFN
                     QIYRNLPFVGVLKPEAYLRTDGQEAGEDEGAETGV"
     gene            complement(4031..5587)
                     /gene="tilS"
                     /locus_tag="VFH60_00635"
     CDS             complement(4031..5587)
                     /gene="tilS"
                     /locus_tag="VFH60_00635"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET6312313.1"
                     /translation="MSVLAQSVARNARQARLSRVLLEAPLVVGVSGGADSLALLSVLR
                     ELRGARAAGTLHVAHLDHGFRGQDGAADARFVETLANEWGLAHTVAYFDVPAYAASRK
                     LSPEDAARRVRYALLAGVAQRHGGTVAVAHNADDQAETVLMHLLRGSGVTGLAGMRLL
                     SDLPIDASYPGLASLMPEVAPKSVRIFRPLLSTWRAEIEAYCQEAGLAPRLDATNLDP
                     AYRRNFIRHNVLPTLEAASPQVKRHLARLADIAAAEDDVLQGITHEEWERLGLAVEDA
                     GIVAPVAAIAALPLGLARRVVRLMLQTVAGHAEDLDFLQVEAARQVLTGAPGSPPSVD
                     LTGGLRVRHVGGTGVVERRGADQVARIFRESADRPLVHAGTFTRVEISNELLLANGWS
                     ATTRVIELGEVAPPPGDYLALFDLDSLASDPEIVLRTRATGDTIQPLGLRGRKKLQDV
                     LVDAKIPWEMRDYLPVVALSGPGAEVLWVPGPGGRRSALAPITGHTRRLLCIEFQPPP
                     EASQEAEGSA"
BASE COUNT         1020 a         1799 c         1943 g          923 t
ORIGIN      
        1 tctactttat cggttcgtac agccctgacc tgatgggcaa cgtggggcac gaacaggcga
       61 tactggccct ccagcggcgg gggcacgaag tagaggtgct gacccagata aacgagccgg
      121 gcgttcccag gtacagccgg acggcctacg caggcgttcc tacttaccgg gtgaacctgg
      181 cgacgttcag tgggcgcgta agtgggctgc tgaggcgcgc ggccggtcgc ttcctccagt
      241 acgagcacac cttcaccctg cttgccgccc tacgccgtca cctgaagcgc catcgttacg
      301 acctgctgca cgtcgagggg gtctacccgt tcggggcggc ggccgccctg ggtgccggga
      361 aaacgcctta cctggcgaac gcgcagggcg cggacgtgat tgagctgccg gagaacgact
      421 acggctacag gcgtttccgg ctgcctcgtg ccgccgtggg gttggcgctg cgcagggcgg
      481 ccctggtgcg cgtcaactcg cccttcatga ttgggtacct ggttggggag cggctggcgg
      541 agcgagagcg catcgtcatg gtgccgcgag tgctggagga cagcgcgttc cccccggtcg
      601 atgcgccgct gcatgagttc aggcgggaga gcagggcgcg cttgtcggag cgttacggga
      661 tagggctgcc taacccggtg gtcatgtcgc tcagccgctt gcacccgttc aaggggcttg
      721 agtacctggt ggatgccatg cccctggtgg cggcggagat gcgggcgata ggcaagccgg
      781 tgccctggtt catactgtgc gggccttcca ggagcaccga gcactacggc gactaccgag
      841 agttcctgca aaagagggcc gccgaactgg gggtagcgca acacgtcatt ttcaccgggc
      901 aggtggccca ccccgacgtg cgccggcacc tggcaggagc ggatatcgta acctcgccca
      961 gcgtggtgga ggcgctgcat cgcgtggccc tggaagcggc ggcggtgggc acccccacgg
     1021 tggtgagcga gacaaccggg gcggcctcct acctggcccc gcacgatgcg tgcatggcgg
     1081 tggcccctcg cagcccgcaa gccatcgccg acgctatcat ccgccttctg agcgaggcca
     1141 acctgtacga gcgggtgagg gacaacgggc tgaaggtggc cgaggggctg cgagtggagc
     1201 cggtagcgga ccaactggaa gccgcgtggc tgcgggcggc ctcggcaggg aagggctagg
     1261 gctggcgtac gacagcgagg tgggtcgtgc caatagccag cttgcgctcg tcgccggggg
     1321 ggttctccgg cggctcgtgg accctcaaga gcaggatgtg gttgccctct tgcagtcgtc
     1381 ccaggtcgat atcgaggtcg gagaacgcgg gggtgagcga ctgttccgag atggccctgc
     1441 cgtccagttc tacagtgacc gtgcggggct tctcgtatga gtaggcggtc atttgcaggc
     1501 ggtaggagcc ttcggcggtg ggcgggatgc caatccacac gagcgcgttg ccgtcggtga
     1561 cgttgcggcc ggtccacctg tgagtggggt caggctcgac cgcgtgccaa ccgctaccca
     1621 gccctacgag cggtagtgtc gcccccggtg ctgcctgccc ctcggcggcc tcgtagatag
     1681 ccacctcctc atcctcgtag gcggggcttt gacctccgaa cagcgcctcc aacacttcct
     1741 ccagcgcgcc gtaacgaccc gtagctaagt cggccttgtg caccacaacg tagcgtatgt
     1801 cgtagtagtg gaggctttcc gaggcagtct ccgaagcagg cggcgcgagc acgtcttcac
     1861 caaccagggg cgacgggtcg gcgctcacca gctgcgccag gccgggggcg gcgtccgcga
     1921 aggggtaggg gaagtgccga gacgtgtagc ctcccactat gggacgctcg tgggccgtct
     1981 ggtgcaggag ggacagggcc gaatactcct cgtagggcag ctctatgaca gcccccggct
     2041 gggtgttcga cctaaggaag gcgacggcac ctccctcttg aggggctaca agctgcctgg
     2101 gcaggccgaa tagctccaac gcgacgagta gcagcgcccc ggcagtgaca ccaacacgca
     2161 aacctgtgcc acgcgtgcgg cctgctaacc acgcaaagcc gtacgccgac atgagcgaca
     2221 gcaccatacc cgccgtcgcc aggaacctca acggttggcg cgacgctcca atgaaaggca
     2281 cgttgtttag cagggcgtag ggcatgggta tgcccgtctc actgccctcg atctttagca
     2341 ccgggccgag cgacatgacc agcagcgcga taaatgccac ccccagcggc agggtgagct
     2401 tccggggcgc tgccaggccc aggagcgcca gcacgatagc gacgtacccc agggccacgg
     2461 tgctgttgtg ggcgttcagg tcgggcagtt gaaggcgggg tggggcgaac agggccagca
     2521 cgtccaccga gttgaaaatg gtggtcctgg gggcggggag catgtaggtc gcgcccgaaa
     2581 gccgctcgcc tatcatggcc cacagcagag ggctgagcag cacgaggaag agggccacac
     2641 ccactcccac cttgagcgcg gcgttccaga ggcgagcacc atattgccga acgccgcgcg
     2701 gctcctgacc aagcaggtag accggcacga agaaggcagt gaacaccact agccccgccg
     2761 tgaagtacca tgtagcaagc gaggccccga cgaggcacag ccccgcgcag acggccgccc
     2821 gcctcccgcc acctggcttc caaaccaggc cggggctgtc gagcgcccgc cacagggcca
     2881 gtatgtaaag cggcacccac cagaccgtca cctcgtccag ttgcccgaag tcgagggagg
     2941 tgccgagcag cggcgtggag gcgaacagcg cgccccccac aagccccgcc tccacagacc
     3001 ctgtgcgctc acggcacagc cagtaggcgg ccagcccgtt tagcgtgagg tttgccagcg
     3061 taagcaggtt gaaggcggcg gtcaggttga agaggcgctg gaaggggatg gagacgaggg
     3121 cgttgagcag gtcgagggag tggtaataga ggttgaggcc ggtggggtgg tagaggtagg
     3181 tggtctggta gggattctgc tggaggtcca cgacggcctt gtccacccac cagaggtccc
     3241 acaggtgcag ccacgcatcg ctaccgcgcg agaggactac cgagtcactg aggtgcagga
     3301 cgaggggata cgagaaggcc acggcccagg cgatgaagaa gaggaggggg aagaaccggg
     3361 cgaacctggc aaagcccctc cccgtgccgc tagactccgg tttcagcgcc ctcgtcctcg
     3421 cccgcctctt gcccgtcggt gcgcaggtac gcttcgggct tgagcacgcc cacgaaaggc
     3481 aggttgcggt aaatctggtt gaaatcgagg ccgtagccta ccacgaactc atccgggatg
     3541 tcgaagcaca cgtaatcgac gcccacgtcc acgcggcggc gggcgggctt gttcaggagg
     3601 gcggccacgc gcaccgaggc ggggttgcgg gtgcgcaggg tgtcgaggat gtagttgagg
     3661 gtgaggccgc tgtcgatgat gtcctccacc acgagcacgt gcttgccctc gatggagcta
     3721 tcgaggtctt tgagtatgcg cacgatgcca ctcgtttcgg tggacgcgcc gtaggaggcc
     3781 acggccatga agtcaatcgt gagaggcaag tctatagcgc gggcgaggtc cacgatgaac
     3841 atgatcgcgc ccctgagcac gcccacgagc agcaggtcct tgcctgcgta ctcccgcgtg
     3901 agggtgcggc ccaggtccgc gatggcgctt ttgaggtctt cctcggtgac gaggatgtgg
     3961 tgaatatcgc cgctcatgtc cctgtacgtt ggtgacacgg gggcctcctc gacatagtct
     4021 aattcgcttc tcatgcgctg ccttccgcct cctggcttgc ctctggtggt ggctgaaact
     4081 cgatacaaag taaacggcgc gtgtgcccgg taataggagc gagtgcggag cgcctgccgc
     4141 cggggccggg cacccacagc acctcggcac cggggccgga gagggccaca acgggcaggt
     4201 agtccctcat ctcccagggg attttagcat caaccagcac gtcctgcaac ttcttgcgcc
     4261 cgcgcagccc cagcggctgt atggtatcgc ccgttgccct agtgcgaagg acaatttccg
     4321 ggtccgaagc gagggaatca aggtcgaaca gggccaggta atcgccggga ggcggcgcca
     4381 cctcgcccaa ctctatcact cgcgtagtgg cgctccagcc gttcgccagg agcagctcat
     4441 tactgatctc cacccgcgtg aaggttcctg cgtgaacgag cggcctatcc gcgctctctc
     4501 taaaaatgcg cgcaacttgg tccgcacccc gccgttccac cacgcccgtt ccacccacat
     4561 gcctgacccg taaccctcca gtcaggtcga ccgaaggcgg actaccggga gcgccggtca
     4621 ggacttgccg ggccgcttca acttgcagga aatccaggtc ctcggcatgt ccagccacag
     4681 tctgcaacat gagcctcacg acacgccgcg ctaaaccgag cggcagggct gcaatcgccg
     4741 ccacaggcgc gacaatcccg gcatcttcga cggcgaggcc aagcctctcc cactcctcgt
     4801 gtgtgattcc ttgcaacacg tcgtcctcgg cagcagcgat atcagccagg cgagcgaggt
     4861 gacgcttgac ctgtgggctt gccgcctcaa gagttggcag cacgttgtgg cggatgaagt
     4921 tgcggcgata ggccgggtca aggttggtgg cgtccaggcg gggtgcaagg ccggcctcct
     4981 ggcagtacgc ctcgatttcg gccctccatg tgctcaggag tggccggaag atacgcacgc
     5041 tctttggagc aacctctggc atcaggctcg ccagcccagg gtagctcgca tcaatgggaa
     5101 ggtcgctcaa cagccgcatg cccgccaggc cggtcacacc tgagccacgc aacaggtgca
     5161 tgaggacagt ttcggcctgg tcgtcagcgt tgtgggctac cgccaccgtg ccgccgtgcc
     5221 gttgggccac gccggcgagg agggcatagc gcacccggcg ggctgcgtct tcgggggaga
     5281 gcttgcggga ggcggcgtag gcgggcacgt caaagtaggc gactgtatgt gcaagccccc
     5341 attcgttcgc gagtgtctcc acaaagcggg catcggcggc cccgtcctgg ccccggaagc
     5401 cgtggtccag gtgggccacg tgcagcgtgc cggccgccct agcgcctcgt agctcccgca
     5461 acacgctcaa gagggccaac gagtcggccc ctcccgacac gccgacgacc aggggggcct
     5521 ccaacagcac cctcgacagg cgcgcttgcc gggcattcct tgcgactgat tgggccagca
     5581 cagacattgg gggtgtctta actcctgcat gagctacctg ttggacgggt cgtcctcccc
     5641 ggtggcacaa cgtgggcaaa cttggggagg agtgggcaca tttta
//
LOCUS       DASRWZ010000048        14830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_277471, whole genome shotgun sequence.
ACCESSION   DASRWZ010000048 DASRWZ010000000
VERSION     DASRWZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 14830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14830
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277471"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFH60_00640"
     CDS             <1..196
                     /locus_tag="VFH60_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312314.1"
                     /translation="GSTFYDGVQRLALYNVVEGYPCGGTGEPCTNGAGTLYFRPGKNA
                     TRGQVAKMVSSAFYPACDAP"
     gene            563..4732
                     /locus_tag="VFH60_00645"
     CDS             563..4732
                     /locus_tag="VFH60_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6312315.1"
                     /translation="MSHKEHQVKIRRMGLLLALLLGVITAALVLRYQQPSSTSTSTAG
                     QNASKAASGVGDESLRGVIMPGDSASRETEALRGREQYFLSRYTYPVGYFDGKWLQEA
                     HEQVAKMQSGIPAGEVVYDRSKSQAPLALSPNGMTSLGPEPGQADGCFVCFNYGLVSG
                     RINSVAIDPTMTSTVYLGVSNGGVWKTTNCCHEGTSWSPVTDDPAISTLTIDEVTLDP
                     TNSNIIYVATGDFRAVGAARGAQGILKSTNGGATWSVLGSNIFTPTRGTAVSGSGTYN
                     AVGAVKVDPNNPNALIAGTKNDLWVSYDAGTNWTGPCYTSAFTNTFGTQRQDNTEILM
                     RDNGSTTTVYYAIGFASGTANGANGVYSATMPASGCPSFTLISRGDNGWPADTGNGTA
                     SNSKPGRIDLAIAPSNPQIIYAVASNASTLGVLGFWRSTNGGETWVQRSTGDALIDCS
                     PDLESANYGQAFYDQAIDVDPNNPDAIVFGEVDTFRSMDGAQSFQNIGCVYTGGDWIH
                     PDQHDFRYMPGSSNQVLFGNDGGIWFTNNANVVSPTRPTIISMNSSVNTMEFYNGDIT
                     GNFATGPEPGANGGTQDNGSFVNVWEGGPGSLGPEEWQLRIGGDGFFAAIEPVNNQTY
                     YQSQNYSVIWRSTQGGYGPYLPVSPGGYDAPWLLDNRSFAMPFEIYKHDCPATGCTHL
                     IAGTYRVWETTNGAQDQDSWYINSPFLTDSQPVCQNPLSLGWACAPFINQLSYAVSMS
                     TTAIAGTSDGNVWYGFNLGQGVQMSAQWVNVSNSNAVLPNRPILDVTTDPMTPTFGDA
                     SIGGFDQATPSTPGHVFRVTCSGLLCASRTWENKTGNLPNLPVDSILVNPRFRQQVFV
                     GTDSGLFFTNDIDAATPVWTRFTTGLPNVWVADLTTDRGFTTLAVWTRNRGLYAWPLP
                     NAPFEQPTSTPTVTGTPPTATSTSVVPTVTPGACFNYSVITRTGASLIPATNNTGVFC
                     DDCAAQIQLPFPVTFYDKTFTQGYALTNGNFTFGPANLAYGDDCFPTRNESYSIHAYT
                     TDLCTNNCVDTEDPNPDPCVGCGIFTATTGTAPNRQFIVMWDAKYFSGANGLRATFEI
                     IFTEGSSDVVVIIGDTGDDGASANSGVQRTANTQYIDYSCQEETLTSGLRIEYKLAQC
                     PTGPPTATPTACPIQFQDVPSSTDVSSFYPYVRCLACRGIVSGYPCGGTNPETGLPEP
                     CGNSNNPYYRPANLITRGQISKIVSEASGLSASPGAQIYEDVAPNSPFFQWINRLSAV
                     GVMGGYPCGGPGEQCGVGNRPYFRPGANATRGQMSKIVANAAGFDEPVSSQSFEDLPP
                     SNSPSSYYPFVERLFLRGIVGGYPCGGAGEPCQPEGRPYFRPGSSVTRGQAAKIVANT
                     FFPNCQSPARP"
     gene            complement(4729..7584)
                     /gene="gyrA"
                     /locus_tag="VFH60_00650"
     CDS             complement(4729..7584)
                     /gene="gyrA"
                     /locus_tag="VFH60_00650"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET6312316.1"
                     /translation="MEIGMIRTVNLDTEMRSAYLDYAMSVIVSRALPDVRDGLKPVQR
                     RVLYAMNQMGLRHNVPYRKSAGIVGEVLKEYHPHGDASVYDALVRMAQDFSLRYPLVD
                     GQGNFGSVDGDPAAAMRYTEVRLRQIAEELLVDIDKDTVSFIPNYDEKAMEPTVLPAR
                     IPSMLLNGAAGIAVGMATNIPPHNLAELCDAEMFLIDNPDATVEELMKFVPGPDFPTG
                     GIIVGNEGIRDAYATGKGRVVMRAKAYIEESRAGRYSVIVTELPFQVNKATLHEKIAE
                     LISDDKLPGASLVRDESDRNGMRLVIELKREAQPKKVLNLLFKHTAMQSTFGVNMLAL
                     VNGNEPRVLTLKKALQHHIEWRQEVITRRTQFELKKAQERAHILEGLKIALDNLDQVI
                     STIRGSRTPESAKANLRSTFKFSEVQAQAILDMRLARLAALERAKIENEYIGVIKEIG
                     YLEDLLAHPQKRYGLIKQDLKELKEKYGDARRTRIAESSSTEFSEEDLIPDLQVLVTI
                     TDRGYIKRLPHDTYKRQNRGGKGIIGMVTREMDAVQHMITCNTLDNLLFLTDKGKVYQ
                     LKAHEVPDTSRQAKGLPLVNLVSLEPGELVTGLIAVRDFKADGAYLVLATRQGKIKKT
                     PLTDYSAVRSTGIIALNLEPGDSLAAARLSHGNSDVLVATRKGQAIRFAETDVRPMGR
                     ATAGVSAIRLAKGDDVVSMDVVRPDSKASLLIVSKKGIGKRTLVKGFPRQGRAGGGVR
                     AMGLTDKSGEVAIARMVFPDDDLVVISTSGQVIRMFADSIPHKQRPAQGVVIMNMKGK
                     DEVASIARIPRTDATGKLLGDDDLEVLTEDQVYDGLDGDGTEGLVVTADGDGLATTNK
                     GRGQGKAMTDALNATVTPSEHVEGNGTKRKAGTAPSAKSNGKTPPPPVDIKTRQRKKE
                     SADRLTIKNTSPPGEVYLIVSRSGACPGQEVSWPGCC"
     gene            8122..10515
                     /locus_tag="VFH60_00655"
     CDS             8122..10515
                     /locus_tag="VFH60_00655"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6312317.1"
                     /translation="MQEVTTFGRWLKSQRQALDLTQERFADRVGCTMSAIEKIERGVR
                     RPSQQVAERIAEVLAIAPAERQAFMAWARGLQAGHEPALPPSVAAAASQAQQPSTSVP
                     IDLPASLTPFIGREAEVAKVRAMLWHTGVRLVTLTGPPGIGKTRLGLAVAASLSQDQE
                     HGVRFVPLDAVRDPGMVVAGIAQALDIEETPGVTAEAALHGYLRDKRLLILLDNFEQV
                     AEAGTAVAEMLAAAPGVKVLVTSRIPLRVYGEHEFPVPPLPLPDLESTDPTSLAHNPA
                     VTLFVQRAQAVQPDFALDSDNGAFVAEICVRLDGLPLAIELAAARSKLFTPQMMLARL
                     GNRLDLLRPGPVDRAARQRSLHGAIQWSYDLLDAREQALFRRLGVFLGGFTVEAAQVV
                     AGEPAGEPDMLEQLESLADKSLLRGQGVGDEPRFGMLETLRDYAMKRLAEAGEGTEVQ
                     QRHARYFLALAERAEPEIRGAQQLAWVERLEREHANLRAALEWSLAPNGDLELGLRLA
                     SALHLFWWTRNYLTEASQWLSRVLSHPEAATPTLVRTKALNAAGTIALFRGDYTQARL
                     FYEECLAMRRELMSQGIGDMGGVAGALGNLGLVAWSLDDHAAAYALTRESLEVLRELG
                     DRHGVARSLTGLGAIAYSQGNHAEAKASYTEALQLFEEFGDRQSVAMMLNNLGEIALA
                     QSNLDEANAYFKDGMVELRDIGNKAAVGGCLDGLAQIAAAEGKPERAARLFGAADAIT
                     KATGEAVPLANRAEYAQIVEGVRDSLGKEAFEAAWDEGQAMSLDEAVAYGLEATGPA"
     gene            10595..11620
                     /locus_tag="VFH60_00660"
     CDS             10595..11620
                     /locus_tag="VFH60_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HET6312318.1"
                     /translation="MRNRILGAIFIPLLALALAACDIGPTPPTPTSLPSATPTVPTPT
                     PVPTDTPVPTPTPMPAEFVWRITGEPDEFDNPSGLDIDSEGNLYVVDAGNHRIQKFDS
                     NGKFLAMWGQEGREDGQFIFTYDHPMGNLVLGGVAVDGDDNVLVADLANSRVQKFDPS
                     GRFLGKWGEPGRLQGRLELPYGIDVESTGQVYLSDFTNFMVMKYEADGKHLLDINEFG
                     IPGFLAVDAQDNLYVTDFEAHAVRKFDNTGKLLFSWGEMGAGDGEFTNPLGIAVDARG
                     NVYVGEAGNPRIQKFDSRGRYLAQWGSKGSGDGKFGSIDDLAIDAQGFIYVSDSGGDN
                     VQKFRLK"
     gene            complement(11800..12249)
                     /locus_tag="VFH60_00665"
     CDS             complement(11800..12249)
                     /locus_tag="VFH60_00665"
                     /inference="COORDINATES: protein motif:HMM:NF039385.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HET6312319.1"
                     /translation="MPAITYYNDKDVLITSETINLAGKVYPVSQVKSVKVWEAEPGLR
                     REWPYLALTLFALLLLTVLNLPNLYPENWHNIIGVVLTLDVLFGVGSAGLLLVPLFLK
                     GECGYMVGLRGTFGSSTPLRCNDEQHAQVLLDAIRLAIVNRGAVEAF"
     gene            complement(12371..13354)
                     /locus_tag="VFH60_00670"
     CDS             complement(12371..13354)
                     /locus_tag="VFH60_00670"
                     /inference="COORDINATES: protein motif:HMM:NF039385.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HET6312320.1"
                     /translation="MSISTFTAYQPRTASVPVAELALYADGLVLLTNRRVVFGSRTYW
                     LKNVKTADVVRVTPRKRMLGFLFYVPFVLAYLAFIYLQSAGGRSGDGLSSALLYGGLA
                     LVALGAAFTALRLRRFRSATVCYVRLNNKSRVLATLDERYAVWLAARIVGAWEGATET
                     AEVPFPEVSQARAQGENLYYSDGYAWVTSDRVRLGSREYGVGGVRKLALGQLPSDRFQ
                     WHLVLAVSLLVLSSVLNYLWFTSVDFRRDYFVFYPGDYWILSLILSVGFLVLLLWTLV
                     NVEDATHILQLRGSTGKVDVFASMDSYYCKRLTEVIKSVMREHSRQPTPGG"
     gene            complement(13390..14349)
                     /locus_tag="VFH60_00675"
     CDS             complement(13390..14349)
                     /locus_tag="VFH60_00675"
                     /inference="COORDINATES: protein motif:HMM:NF039385.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HET6312321.1"
                     /translation="MAGQAEWSAGIPAATGPETVLYHAADVWITDTRVVLGGRSYQLR
                     DIQSPEVAAVTPKQRLRARLPTIVPLVLFMAFLLFGNFNGTFLANLWPSISVILLVAL
                     VCIAVFVVFPIGRTRFATYYSVRLTGKEYAYTSTDVEYVRWLAEQIARVKLGASEVEA
                     QPASPPIDAGSGEYVFYSDGRAAVTSKWFVLGSERTQLQDVKRASVTQVPSDKALQLQ
                     SLSSTLLFVSIFLTNIQNSAPNNGRDFYLLFPGDTTLISFICLATVLVLLFVVLSRLR
                     GSAYVVTLRGNFEDAHHTEVFASFDQAYAATLASHINDAISAR"
     gene            complement(14351..14557)
                     /locus_tag="VFH60_00680"
     CDS             complement(14351..14557)
                     /locus_tag="VFH60_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312322.1"
                     /translation="MTSVLPPSASEDDEYALSKKAPARSEHEDYVATPRRTPVPVLVA
                     VLVAALVILGTVLIVMMVWGGRLL"
BASE COUNT         2924 a         4735 c         4503 g         2668 t
ORIGIN      
        1 aggcagcacc ttctacgatg gcgtgcagag gctggccctg tacaacgtgg tggagggcta
       61 cccctgcggc ggcacgggcg agccatgcac caacggggcg ggtacgctct acttccgccc
      121 cggcaagaac gccaccaggg ggcaggttgc caagatggta tccagcgcct tctaccccgc
      181 ctgcgacgcg ccttagagag caggtaacga gagtgttctc ttagaactgc aataagggat
      241 gtcctcaccc agtaagtgag gacatccctt ttgttaaggt gggtatatat tttccctgtt
      301 caaatgtata ttcgaatcga gggcacaatc tgtgctggaa cgtcaaattc ctgctacatg
      361 gtaggggcac aactacccac acgagtggca gagcgttcgc gcacatgccg gcgtccgaac
      421 gcacatcgta aaagaggagt ggctcggtag acctgacacg caattcaggc caaaggcgtg
      481 ggagaacgac gcctgagcgt accactacct ccaccaggcc atcagtctct cggttagtgc
      541 actgcaagga aggagaaagg tcatgtcaca taaggaacat caggtgaaaa tccggcgcat
      601 gggactcttg ctggcgctcc tgctgggcgt aatcaccgcc gcgttggtgt tgcgctacca
      661 gcaaccctcc agcacgagta cctcgaccgc cggccagaac gcctctaagg cagcgtcagg
      721 cgtgggcgac gagagcctgc gcggcgtaat catgcccggg gatagcgcca gtagagaaac
      781 ggaagccctg cgcgggcggg agcagtactt cctcagccgc tacacctacc cggtgggcta
      841 cttcgacggc aagtggctgc aagaagccca cgagcaggtc gcgaagatgc agtccggcat
      901 ccctgccggt gaggtcgtct acgaccgctc taagagccag gcgcccctgg ccctcagtcc
      961 caacggcatg acaagcttgg ggcctgagcc ggggcaggct gacggttgct tcgtgtgctt
     1021 caactacggc ctggtcagcg gccgcatcaa ctccgtcgcc atagacccca ccatgacctc
     1081 gacggtgtac ctgggcgtgt ctaatggtgg tgtgtggaag accaccaact gctgccacga
     1141 agggactagc tggagccccg tgaccgatga cccggctatc agcaccctca ccatcgatga
     1201 agtcacgctc gaccccacca acagcaatat catctacgtg gctacgggcg acttccgagc
     1261 ggtaggcgcg gcccgcggcg cgcagggcat cctcaagagc acgaacggcg gggccacctg
     1321 gtccgtgctc ggcagcaaca tattcacccc tacccgtggg accgcggtca gcggctccgg
     1381 cacctacaat gccgttgggg ccgtgaaagt ggacccgaac aaccccaacg ccctcatcgc
     1441 gggcaccaag aacgacctct gggtgtctta tgacgcgggg actaactgga cggggccttg
     1501 ctacaccagc gccttcacca atacctttgg cacccagcgc caggataaca ccgaaatcct
     1561 gatgcgcgac aacggttcca cgaccacagt ttattatgcg attggcttcg cctccggtac
     1621 cgccaacggt gccaacggtg tctactcggc aactatgccc gccagtggct gcccgtcctt
     1681 taccctcata tcgaggggag ataacggctg gcccgccgac acgggcaacg gcaccgcctc
     1741 caacagcaag ccgggccgca tcgacctcgc cattgccccg agcaatcccc agatcatcta
     1801 cgctgtggcc tccaatgcct ccaccctggg cgtgctcggc ttctggcgct ctacaaacgg
     1861 cggcgagacc tgggtgcaac gctccactgg agacgcgttg atcgattgca gtcccgacct
     1921 cgaatccgca aactacggcc aggccttcta cgaccaggca attgacgttg accccaacaa
     1981 ccccgacgct atagttttcg gtgaagtgga cacgttcaga tcgatggacg gagcgcagag
     2041 cttccagaac atcggctgcg tctacaccgg gggcgactgg atacaccctg accagcacga
     2101 cttccgctat atgccggggt cgtcgaacca ggtgctgttc ggcaacgacg gtggtatctg
     2161 gttcaccaac aacgccaacg tcgtctctcc aaccagacct accattatct caatgaactc
     2221 ctcggtaaac actatggagt tctataacgg cgacatcacg ggcaacttcg ccacgggacc
     2281 ggagcccggt gccaacggcg gcacccagga caacggctcg ttcgtgaacg tgtgggaagg
     2341 cggccccggc agcctgggac ctgaagagtg gcaattgcgc ataggcggcg acggtttctt
     2401 cgcggcaatc gagccggtga acaaccagac ctactaccag tcgcagaact acagcgttat
     2461 ctggcgctcc actcaaggcg gctatggacc ctatctcccg gtgagccccg gcgggtacga
     2521 tgccccctgg ctcctggata acaggtcctt cgccatgccg ttcgagatat acaagcacga
     2581 ctgcccggcg accggctgca cgcacctgat cgcgggtacc taccgcgtgt gggagacgac
     2641 aaacggggcg caggaccagg atagctggta catcaacagc ccgttcctca ccgactctca
     2701 acccgtatgc cagaatccgt tgagcctggg ctgggcctgc gcgcctttca tcaaccagct
     2761 cagctacgcg gtgagcatgt ccaccacggc tatcgcgggc acaagcgacg gcaatgtctg
     2821 gtacggcttc aacctgggcc agggtgttca gatgagcgcc cagtgggtaa acgtcagcaa
     2881 cagcaatgcc gtgctgccca accgccccat cctcgacgta accacggacc cgatgacgcc
     2941 taccttcggc gacgcgtcga taggtggctt cgatcaagct actcccagca ctccgggcca
     3001 tgtgttccgt gttacgtgta gcggcctgct gtgcgcgagc cgcacctggg agaacaaaac
     3061 gggcaacctg cccaacctgc ccgtggactc gatactggtg aaccctcggt tccgccagca
     3121 ggtcttcgtg ggcaccgact ccggcctgtt cttcaccaac gacatcgatg ccgcgacccc
     3181 tgtgtggacg cgcttcacga cgggcctacc caacgtgtgg gtagccgacc tgacgacaga
     3241 ccgtggcttc accacgctgg cggtctggac ccgcaaccgc ggcctctacg cgtggccgct
     3301 gccgaacgcc ccgttcgagc agcctacctc tacccccacc gtaacgggca ctccccccac
     3361 ggctacaagc acctccgttg tgcctactgt cacccccgga gcctgcttca actactcggt
     3421 gataacccgg acgggggcct cactgatacc cgccaccaac aacacaggcg tcttctgcga
     3481 cgattgcgcc gcacaaatac aactgccgtt cccggtgacg ttctacgaca agacgttcac
     3541 ccagggctac gccctcacca acggtaactt cacctttggc ccggctaacc tggcatatgg
     3601 agacgactgc ttccccacca ggaacgagtc gtacagcatc catgcctaca cgacggacct
     3661 gtgcaccaac aactgcgttg acacagagga ccccaacccg gacccctgcg taggctgcgg
     3721 tatcttcacg gcaaccacag gcaccgcgcc caataggcag ttcatcgtga tgtgggatgc
     3781 caagtacttc agcggagcca acgggctgag ggctaccttc gagatcatct ttacggaggg
     3841 ttcgtccgac gtagtcgtga ttatcggaga tacgggcgac gacggcgcga gtgcaaactc
     3901 cggcgtgcag cgcaccgcca acacccagta catcgactac tcctgccagg aggaaacgct
     3961 tacctcgggc ctgaggatcg agtacaagct cgcacagtgt cccaccgggc cacctacggc
     4021 cacacctacg gcctgcccca tccagttcca ggacgtgccc tcgagcaccg acgtgtcgtc
     4081 gttctacccg tacgtgcgct gccttgcctg tagaggcatc gtcagcggct acccgtgcgg
     4141 aggcacgaac cctgagacgg ggctacccga accgtgcggc aactccaaca acccttacta
     4201 ccgcccggcc aacttgatca ccagggggca gatcagtaag atcgtgtccg aggcttcagg
     4261 actgagcgcc agtccgggcg ctcagatcta cgaggacgta gcgcctaact ccccgttctt
     4321 ccagtggatc aaccgcctgt ccgccgtggg cgtgatgggt ggctacccgt gcggcggacc
     4381 tggcgagcaa tgcggcgtgg gcaacaggcc gtacttcagg ccgggggcca acgctaccag
     4441 ggggcagatg tccaagatag tggctaatgc cgcgggcttc gacgagccgg tatccagcca
     4501 gtccttcgag gacctgccac ccagcaactc accctcctct tactacccgt tcgtagagcg
     4561 tctgttcctg cgtggcatag taggaggtta cccctgcgga ggagcgggcg agccttgcca
     4621 gccggaaggt agaccttact tccggcctgg aagctccgta accagggggc aggcggccaa
     4681 gattgtggct aacactttct tccccaactg ccaatctccg gccagacctt agcagcaacc
     4741 cggccaggag acctcctggc cgggacaagc acctgagcgt gagacaatca aatacacctc
     4801 cccgggtggg gaggtgtttt tgattgtcag cctgtctgct gattccttct tccgctgccg
     4861 ggtcttgatg tccacgggcg ggggcggggt cttgccgttg ctcttggccg agggggcggt
     4921 acctgccttg cgcttggtgc cgttgccctc gacgtgctcg gagggcgtga ctgtggcatt
     4981 gagcgcgtcg gtcatcgcct tgccctggcc gcgtcccttg ttcgtggtgg cgaggccgtc
     5041 gccgtcggcg gtgaccacca ggccctcggt cccatcgccg tccaggccgt cgtacacctg
     5101 gtcctcggtg agcacctcga ggtcgtcgtc gcccagcagc ttgccggtcg cgtctgtccg
     5161 tggtatgcga gcgatggagg ccacttcgtc cttgcccttc atgttcatga tgaccacacc
     5221 ctgagcggga cgctgcttgt gcgggatgct gtccgcgaac atgcgaatta cctgcccgct
     5281 cgtggagatc accaccaggt cgtcatcggg gaagaccatg cgggcaatcg ccacttcgcc
     5341 gctcttgtcg gtcagcccca tagcgcgaac gccgccgccc gcgcggccct ggcgagggaa
     5401 gcccttcacc agcgtgcgct tgcctatgcc cttcttggac acaatcagca gcgaagcctt
     5461 ggagtcgggg cgtaccacgt ccatgctcac cacgtcgtcg ccttttgcga ggcgaatggc
     5521 tgatacaccg gcggtggccc ggcccatcgg gcgcacgtcg gtctccgcga agcggatagc
     5581 ctggcccttg cgggtggcta ccagcacgtc gctattgccg tgcgacagcc gcgccgccgc
     5641 gagagagtcg cccggctcca ggttgagggc gataatgccg gtggagcgca ccgcgctata
     5701 gtcggtgagc ggggtcttct tgatcttgcc ctgcctggtg gcgagcacca ggtaagcgcc
     5761 gtcggccttg aagtcgcgca ccgcgatgag gcccgtcaca agctcgcccg gctccagcga
     5821 caccaggttc accaggggga ggcccttggc ctgcctgctg gtatcgggca cctcgtgggc
     5881 ctttagctgg tagaccttgc ccttgtcggt gaggaacagc agattgtcca gggtgttgca
     5941 ggtaatcatg tgctgcacgg cgtccatctc gcgagtcacc atgccgatga tgcccttgcc
     6001 gcccctgttc tgacgcttgt aagtatcgtg gggcaggcgc ttgatgtaac cccggtcggt
     6061 gatcgtcacg agcacctgca agtcggggat gaggtcttct tcgctgaact cggtggagga
     6121 agactccgcg atgcgggtgc ggcgggcgtc gccgtacttc tccttcagct ccttcaggtc
     6181 ctgcttgatg aggccgtacc gcttctgagg gtgggccagc aggtcctcaa ggtagccgat
     6241 ctccttgatg accccgatat actcgttctc tatcttggcg cgctccaggg ccgccaggcg
     6301 ggcaaggcgc atgtccagga tggcctgggc ctgtacctcg gagaacttga aggtgctccg
     6361 caggttggcc ttggcgcttt cgggcgtgcg gctcccccgt atcgtggaga ttacctggtc
     6421 caggttgtcg agggctatct tgaggccttc gaggatgtgg gcgcgctcct gggccttctt
     6481 cagctcgaac tgggtgcggc gggtgattac ctcttgccgc cactcgatgt ggtgctgcaa
     6541 cgccttcttc agcgtgagca cgcgaggctc gttgccgttc accagggcta gcatgttcac
     6601 gccgaacgtg ctctgcatgg cggtgtgctt gaacagcaga ttgagcacct tcttgggctg
     6661 ggcttcgcgc ttcagctcga tcaccaggcg catgccgttg cggtccgact cgtcgcgcac
     6721 caggctagcg cccggcagct tgtcgtccga gataagctcg gcgatcttct cgtgcagggt
     6781 ggccttgttc acctggaacg gcagctcggt cacgatgacg ctgtagcggc cggcgcggct
     6841 ctcctcgatg taggccttgg cgcgcatgac cacgcggccc ttgccggtgg cgtaagcgtc
     6901 gcggatgccc tcgttgccca cgataatgcc gccggtcggg aagtcggggc cgggcacgaa
     6961 cttcatcagt tcctcgaccg tggcgtcggg gttgtcgatg aggaacatct cggcatcgca
     7021 caattccgcg aggttgtgag gcgggatatt ggtcgccata cccacggcga taccggcagc
     7081 gccgttgagc agcatgctgg gtatgcgcgc cgggaggacg gtcggctcca tcgccttttc
     7141 gtcgtagttg gggatgaagc taaccgtgtc tttatcgatg tccaccagca gctcttcggc
     7201 tatctggcgc aggcggacct cggtgtagcg catggcagcc gcggggtcgc cgtccaccga
     7261 gccgaagtta ccctggccgt ccacgagggg atagcggaga gagaagtcct gtgccatacg
     7321 caccagggcg tcgtacacag aagcgtcgcc gtgagggtgg tactctttga gcacctcacc
     7381 cacgataccg gcgctcttgc ggtagggcac gttatgccgc agccccatct ggttcatcgc
     7441 gtacagcacg cgccgctgca ccggcttcag gccgtctcgt acgtcgggca gggcgcggga
     7501 cacgatcacg ctcatcgcgt aatcgaggta cgccgaccgc atctcggtat cgaggttgac
     7561 cgttctaatc atgcctattt ccatgcgtgc aaagcctcca aaactccaaa ctcaagtctt
     7621 ctacacatat tctaaagcag actcttactt acttatatta atgcacttcc acacattatt
     7681 atacccgatt ggaggggaaa gtgccaggtt ttacacatgg aaacagcaaa tatgttctaa
     7741 atatgtgttc atcgctagag gaagctgctc taggagtcat aacggcaata cagatagcgg
     7801 agatgagcac ccagaatccc cctttgtcat tctgagcaca gcgaagaatc tcgtcccaca
     7861 ccgagatgtg gctcctttct tacggcactt tgctgctgta ggaaagcggg cagttggctg
     7921 ctaaaggacg agatccttcg ctacgctcag aatgacaaag gccgctgcga ctaccctgta
     7981 aagacggcat ccgccttcgt ataagttgtg gtatattgcg aatagaagac ctggctatgc
     8041 cgtcaggccc ttgcgctcgc aaagtatggg gaagctgccg ggctaccagg cacgcttgcc
     8101 ctcacgctca gggataggtc gatgcaggaa gtcactacgt tcggacgctg gctcaagtcc
     8161 caacgacagg cgctggacct tacccaggaa cggttcgccg accgggtagg ctgcaccatg
     8221 tccgcgatag agaaaatcga gcgaggcgtg cgccgcccct cgcagcaggt ggctgagcgc
     8281 atagccgagg tgctcgccat cgcccccgcc gagcggcagg cgttcatggc ctgggcgcgc
     8341 gggctgcaag ccgggcacga acctgcttta cccccttcgg tagctgccgc cgcctcccag
     8401 gcgcaacaac cctctacctc tgttcccatt gatttaccgg cctccctgac cccgttcatc
     8461 gggcgagagg ccgaggtcgc taaggtcagg gccatgctct ggcacaccgg cgtgcggctc
     8521 gttaccctga cggggccgcc cggcataggc aagacccggc tgggtctcgc ggtcgccgct
     8581 tccctctccc aggaccagga gcacggggtg cgcttcgtgc cactcgacgc cgtccgcgat
     8641 cccggcatgg tggtggccgg catcgcccag gccctcgaca tcgaggagac ccccggcgta
     8701 acggcggaag cggccctgca cggctacctg cgcgacaagc gactgctcat attgctggac
     8761 aacttcgagc aggtggcgga ggcgggcacg gctgtcgcgg agatgctggc agccgccccc
     8821 ggtgtaaagg tgttagtcac cagccgcatc cccctccgcg tgtacggaga gcacgagttc
     8881 ccggtgccgc ccctccccct gcccgacctg gagagcacgg accccaccag cctggcgcac
     8941 aaccccgccg taacactctt cgtccagcgc gcccaggcgg tgcaacccga cttcgcgctc
     9001 gacagcgaca acggcgcgtt cgtggccgag atttgcgtgc ggctcgacgg gctgcccctg
     9061 gctatagagc tggcggcggc ccgtagcaag ctcttcaccc cgcagatgat gctcgcccgc
     9121 ctgggcaacc ggctggacct gctcaggccc ggccccgtag acagggcggc ccgccagcgg
     9181 tccttacacg gagctataca gtggagctac gacctcctcg atgcgcgaga gcaggccctc
     9241 ttccgcaggc tgggcgtctt ccttgggggc ttcacggtgg aagccgcaca ggtggttgcc
     9301 ggtgagcctg ccggtgagcc ggacatgctt gagcagcttg agagcctcgc ggacaagagc
     9361 ctattgcgcg ggcaaggggt aggcgacgag ccgcggttcg gtatgttgga gacgctgcga
     9421 gattacgcta tgaaacggct ggcggaggcc ggcgaaggca cggaggtcca gcagcggcac
     9481 gcccgctact tcctcgcgct ggcggaacgg gcggagccgg agatacgggg agcgcaacag
     9541 ctggcctggg tggagaggct ggagcgagaa cacgccaacc tgcgggccgc actggagtgg
     9601 agcctggccc cgaacggcga cctggagctg ggcctgcgcc tggcctcggc cctccatctc
     9661 ttctggtgga cgcgcaacta cctgaccgag gcaagccagt ggctgtcgag ggtcttgtca
     9721 cacccagaag cggcaactcc aaccctggtg cgtacaaagg cgctcaacgc ggcgggtacg
     9781 attgccctgt tcaggggcga ctacacccag gcccgcctct tctacgagga atgcctggca
     9841 atgcggcggg agttgatgag ccagggcatt ggagacatgg gcggtgtagc gggcgcgctt
     9901 ggcaacctgg ggcttgtcgc ctggagcctg gacgaccatg cagcggcgta cgcgctcacc
     9961 agggaaagcc tggaagtact gcgggagctt ggcgacaggc atggcgtggc gcgctcgctc
    10021 accggcctgg gcgcgatagc ctacagccag ggcaaccacg ccgaagctaa agcctcttat
    10081 acggaagccc tccaattgtt cgaggagttt ggcgacaggc agagcgtcgc catgatgctc
    10141 aacaacctgg gagagatagc cctggcccag tccaacctgg acgaagcgaa cgcgtacttc
    10201 aaggacggca tggtggagtt gagggacata ggcaacaagg cggccgtcgg cgggtgcctg
    10261 gacggactcg cgcagatagc ggcggcggaa ggtaagccgg agcgggcggc gcgacttttc
    10321 ggggcggccg atgccataac gaaggccacg ggcgaggcgg tgccgctggc taatcgcgcc
    10381 gagtacgccc aaatcgtgga gggcgtgcgc gacagcctgg gcaaagaggc gttcgaggca
    10441 gcctgggacg agggccaggc tatgtcattg gacgaagccg tcgcctatgg gttggaggcg
    10501 accggccccg cctgaaccgg tatgggcctg gaccggtatg aatatgtata cgggaggtct
    10561 tgtcaaaacc gacacctggt gaggagcaca cgccatgcgg aaccggatac taggagcgat
    10621 tttcataccg ttgctggccc ttgcgctggc ggcgtgcgac atcggaccca caccacccac
    10681 ccccacgtca ctaccctcgg ctacccccac cgtacccact cccacgcccg tgcccaccga
    10741 cacccccgta ccgaccccta cgcccatgcc cgccgagttc gtgtggagga taaccggcga
    10801 gcctgacgag ttcgacaacc cctccggcct ggacatcgac agcgagggca acctctacgt
    10861 ggtggacgcc ggtaaccacc gcatccagaa gttcgattcc aatggcaaat tcctcgctat
    10921 gtggggccag gaaggacgcg aggacgggca gttcatcttc acctacgacc accctatggg
    10981 caacctcgtg ctgggcggcg tggcggtgga cggtgacgac aacgtactcg tggcggacct
    11041 tgcaaactcc cgcgtgcaga agttcgaccc ctccggccgg ttcctcggca agtggggcga
    11101 accgggccgc ctccagggta ggctggaact cccttatggc atcgacgtgg agagcacggg
    11161 gcaagtgtac ctcagcgact tcacaaactt catggtgatg aagtacgagg ccgacggcaa
    11221 gcacctcctc gacatcaacg agttcggcat ccccggcttc ctcgccgtgg acgcgcaaga
    11281 caatctctac gtcaccgact tcgaggccca cgcggtaagg aagttcgaca acacgggcaa
    11341 gctgctattt agctggggcg agatgggcgc gggcgacggg gagttcacca acccgctcgg
    11401 catagcggtc gatgcgcggg gcaacgtgta cgtaggcgag gcgggcaacc cgcgcatcca
    11461 gaagttcgac tccaggggcc gctatcttgc gcaatggggc agcaaaggca gcggcgacgg
    11521 caaattcggc tccatcgacg acctcgccat agacgcgcag ggcttcatct acgtcagcga
    11581 ctccggcggc gacaacgtgc agaagttccg gctgaagtaa gagaggaaac gaaaacgcaa
    11641 agacgcaaag acgcaaagaa cgcgcagaga ttaagttaga ggtgttctaa gcaaaaggac
    11701 cagggtacgt cagatgtacc ctggtccttt tgcactaact aacccttatc ctctttgcgc
    11761 gttctttgcg tctttgcgtc tttgcgtttt cgtctaccat tagaaagcct ccaccgcccc
    11821 acggttgacg attgccaggc gtatggcgtc cagcagcacc tgggcgtgct gttcgtcgtt
    11881 gcagcgcaag ggggtagaac tgccgaaggt gccgcgcaag cccaccatgt agccgcactc
    11941 gcctttgagg aagagcggca cgagcagcag gcccgccgag cctaccccga agagcacgtc
    12001 cagcgtcagc accaccccta tgatgttgtg ccagttctcg gggtagaggt tgggcaggtt
    12061 gagcacggtg agcagcagca gcgcgaaaag cgtcagcgcc aggtacggcc attcgcgccg
    12121 caggcccggc tcggcctccc acaccttcac cgacttcacc tggctgaccg ggtagacctt
    12181 acccgccagg ttgatagtct cgcttgtgat aagcacgtct ttatcgttgt agtatgttat
    12241 agctggcatg ttattttgcg ttccccttcg cattttacat acgcataagg gtaacaggcc
    12301 gtgtcacgcc gccgggtgct actgctagcg cgaagaggat acaggtcatg ggtctacagg
    12361 ttaaccttca tcaaccgccg ggcgtcggct ggcgactatg ctccctcatc accgacttga
    12421 tgacttccgt aagccgcttg caataatagc tgtccatcga agcgaagacg tccactttgc
    12481 ccgtgctgcc tcgcagttgg aggatgtggg tggcatcttc cacgttcacc agcgtccaca
    12541 acagcagcac caggaagccc acggacagta tcagcgacaa tatccagtag tcgcccggat
    12601 agaagacaaa gtagtcgcgc ctgaaatcga cggaggtgaa ccacaggtag ttaagcacac
    12661 tactcaacac cagcagcgac accgccagca ccaggtgcca ctggaagcgg tcggagggta
    12721 gctgccccaa cgcaagcttg cgaacgccgc ccacgccgta ctcgcggctt cccagcctca
    12781 ccctgtcgct cgtcacccag gcgtaaccgt cggagtagta gaggttctcg ccctgcgccc
    12841 gcgcctgcga cacctccggg aacggcacct cagcggtctc cgtcgccccc tcccaggccc
    12901 caacgatgcg cgccgcgagc cacactgcat accgctcgtc cagggttgca agcacccggc
    12961 tcttgttgtt tagcctgacg tagcacaccg tggccgaccg gaacctgcgc aggcgcaagg
    13021 cggtgaaagc ggcccccagc gccacgagcg caagcccccc gtacaggaga gcgctactca
    13081 acccgtcgcc ggaccgtccc ccggcgcttt gcaggtaaat gaaagccagg taggccagga
    13141 cgaagggcac gtaaaagaga aatcctagca tgcgcttgcg gggcgtgacc ctgaccacgt
    13201 cggcagtctt gacgtttttc agccagtagg tccggctgcc gaacaccacg cgcctgttgg
    13261 tcagcagcac cagcccgtca gcgtagaggg ccagttccgc gacgggtacg cttgcggtac
    13321 gcggctggta ggctgtgaac gtcgagatgc tcatgaagat gtggcggcgc tgcccgacaa
    13381 aatatcggac tagcgggcgc tgatggcgtc attaatgtgg ctggcgagtg tcgcggcgta
    13441 ggcctggtcg aacgaggcga acacctccgt gtggtgcgca tcctcgaagt tgccacgcaa
    13501 cgtcaccaca tacgccgacc cgcgcagcct gcttaagacc acgaacaaca ggaccagtac
    13561 agtagctaag caaatgaagg agatgagcgt agtgtcgccg gggaacagca ggtaaaagtc
    13621 acgaccattg tttggcgcgg agttctggat attggtcaag aagatgctca caaacagtaa
    13681 cgtagagctg agactttgca gttgcagggc tttatcggag gggacctgcg ttacgcttgc
    13741 tctcttgaca tcttgcagtt gcgtacgctc gcttcccaac acgaaccact tgcttgtgac
    13801 ggcggcccgt ccgtccgagt agaacacgta ctcaccggag ccggcatcta tcggcggcga
    13861 agcgggctgg gcctccacct cgctcgcacc cagcttgacg cgggctattt gctctgccaa
    13921 ccagcgcacg tattcgacat cggtagaagt ataggcgtat tccttgccgg tgagccttac
    13981 cgagtagtaa gtggcgaacc gggtccttcc tattggaaag actacaaaga ctgcgatgca
    14041 caccagcgcc accagcaata tcacggagat agagggccag agattagcta agaaggtgcc
    14101 gttgaaatta ccaaagagca gaaaggccat gaagaggacg agcgggacga tagtcggaag
    14161 gcgcgcgcgc aatcgctgct tgggagtcac cgcggctacc tccggcgatt gaatatcccg
    14221 gagctggtaa gacctgcccc caagcaccac gcgcgtgtcc gtgatccaca cgtcggcggc
    14281 gtggtacaga acggtctcag gacctgtagc ggcgggtatg cccgcgctcc attcagcttg
    14341 cccggccatg ctacaacaac ctccctcccc acaccatcat cacgataagc accgtaccca
    14401 gtatcaccag cgccgccacc agcacggcca cgagcacggg cacaggggtc ctgcgcggcg
    14461 tggcaacgta gtcctcatgc tcggagcgcg cgggcgcttt cttgctgagt gcgtactcgt
    14521 cgtcctcgga ggccgagggc ggcagcacag aagtcataat gatacctcac aagaaaccaa
    14581 aagggcggac tccccgtccg ccccattata ctagacccta ttgctctcga atacgaatcc
    14641 tctttgcaaa gtcaaggaag agagaagtgg gggacacccc cagccccgtc aggggagtac
    14701 ccctgcaccc ccctggtggt ttctcctcct attgcccccc gctggccaat gtagccacca
    14761 actgcggcag ggagatgcgg gcggcttcga tgagggggcc ggagaagatg aagaagagga
    14821 tcacggctat
//
LOCUS       DASRWZ010000049         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1168363, whole genome shotgun sequence.
ACCESSION   DASRWZ010000049 DASRWZ010000000
VERSION     DASRWZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168363"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFH60_00685"
     CDS             <1..542
                     /locus_tag="VFH60_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6312323.1"
                     /translation="KGRRFKQEVLAVRYRGYDIAEVLDLTIEEALAVFQDIAAVAARL
                     SLMSEVGLGYLQLGQPANTLSGGEAQRIKLAKELARRTTGRTLYLLDEPTTGLHPADV
                     VRLLDLLQRLVDGGNTVVVIEHHIDVIRAADWIIDLGPEGGEAGGYVVAEGTPEAVAG
                     VAQSSTGRFLDRALEGAAL"
     gene            complement(597..1562)
                     /locus_tag="VFH60_00690"
     CDS             complement(597..1562)
                     /locus_tag="VFH60_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6312324.1"
                     /translation="MQTRYDTRTLICIGLTLVLWASGFAGIRAGLSGYDPATMALLRF
                     AVASVALALYAVVTRVRLPARQDLPRLAIAGFLGITVYHLALNYGERSVSAGAASLLV
                     ASAPVWTSLLATLFLGERLKLLGWAGIGLSFLGAALIGLGQGDGLSLEPGVGLVLLAA
                     LAQGSYFVIQKPALRTYRPIEVVSYVTWVGTLVLLVFLPGLVADVQTAPLDATLAVVY
                     MGIGPGAVAYAAWAYVLSRMQASTASSLLYIVPVMAFTIAWFWLGEVPTYMSIVGGAL
                     ALAGVALVNKKPRHDGKDRAANVSHTNPGRDVPPLTARAEPSQAE"
BASE COUNT          361 a          573 c          633 g          297 t
ORIGIN      
        1 gcaaagggcg gcgtttcaaa caggaagtgc tcgccgttcg ctatcgcggc tacgacatcg
       61 ccgaggtact cgacctgacg attgaggagg cgctggcggt attccaggac atagcggcgg
      121 tggcggcgcg gctatccctc atgtccgagg ttgggttggg gtacctgcaa ctggggcagc
      181 ctgccaatac gctgtcgggt ggagaagcgc agcgcatcaa gctggctaaa gaactggcgc
      241 gccgcacgac gggtcgcacg ctttacctgc tggacgagcc aaccaccggc ctgcaccccg
      301 ccgacgtcgt ccggctcctc gacctgttgc aacggttggt ggatggcggc aacaccgtcg
      361 tggttattga gcatcacatc gacgtcattc gcgccgccga ctggattatc gacctggggc
      421 cggagggtgg tgaggcgggc gggtacgtcg tggcggaggg gacgccggag gcggtcgccg
      481 gggttgccca gtcgtctacg gggaggttcc tggaccgggc gcttgaaggg gccgctctct
      541 agaaaggcta ggctgaagcc tgaggcaaag gcaacgccgg agaaagccca ttgccgtcat
      601 tcggcctgtg agggttctgc cctggcggtc aggggcggca cgtcacgacc tgggttggtg
      661 tgtgacacgt tagcagcccg gtccttgccg tcgtggcgcg gcttcttgtt gaccaacgcc
      721 acgcctgcca gggccagcgc gccgccgact atcgacatat aggtgggcac ctcaccgagc
      781 cagaaccagg cgatagtgaa ggccatcacg ggcacgatat agagtaagct gctggcggtg
      841 gacgcctgca tgcgagagag cacgtaggcc caggcggcgt aggccaccgc gccaggtcct
      901 atgcccatgt agaccacggc gagggtcgca tccagcggcg cggtctgcac gtcggctacc
      961 agccccggca gaaagacgag cagcacgagc gtgcccaccc aggtgacgta ggacacgacc
     1021 tctatcggcc ggtaggtgcg cagagcgggc ttctgtatca cgaagtacga accctgggcg
     1081 agggccgcga gcagcaccag ccctacccca ggttccaggc tcagcccgtc gccctgccca
     1141 aggccaatca gggccgcgcc caggaagcta agcccgatgc cggcccaacc gaggagcttg
     1201 agccgctcgc ccaggaagag ggtagccagc agtgaggtcc agacgggcgc ggaggccacc
     1261 aggagacttg ccgccccagc ggacacgctc cgctcgccat agttgagggc caggtggtag
     1321 accgttatgc cgaggaagcc cgcgattgcc agccggggga ggtcttgccg ggcgggcagt
     1381 cgcacccgcg tgacaaccgc gtatagggcc agcgccacgg aggccacggc gaagcgcagc
     1441 agggccatcg tagccgggtc gtagcccgaa agccccgcgc ggatgcccgc gaaccccgac
     1501 gcccacagca cgagcgtgag gccgatgcaa atcagcgtcc tggtgtcata gcgggtctgc
     1561 atggtgggta atatctctct ttctgtttgt agatgcgaat ggttgacagc agggtagaac
     1621 cgcccaaagt tgcctctgtc atcctgagcg tagcgaagga tcagtggccc gctctcgacc
     1681 cttggtatcg atggaccagt gtttctaatg ccagcaccgt ccccgatgtt gaagactctc
     1741 gcctacccac ctgatcctta gcggagttta ccctgagcgc agccgaaggg ctcaggatga
     1801 caaagggtaa tacaggctga cgggagagac tcaggacgac aaagccccca cttttgaacg
     1861 catg
//
LOCUS       DASRWZ010000050         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_894367, whole genome shotgun sequence.
ACCESSION   DASRWZ010000050 DASRWZ010000000
VERSION     DASRWZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894367"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1964)
                     /locus_tag="VFH60_00695"
     CDS             complement(<1..1964)
                     /locus_tag="VFH60_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET6312325.1"
                     /translation="MSSSWKDINYLNFDITIQRAGNGYRAHLHSQSGENVTVTFGKPF
                     SDMELENFLLKAGYSRRSVRRIDTVETAAAKEFGGKLFNTLFKGDAQECLRRDLSYAQ
                     QQDARLRLRLSLTEAPELADLPWEYLYNPANNKFLALSVETPVVRYLELRERIAPLTV
                     NPPLRVLMMVSSHSDFDALDTQAEAKNLHSALADLEARGLVEIETMEDANLAALQRKL
                     RRGEYHIFHFIGHGGYDHQTEQGVLILEDDNGRGRAVSGQDLGMLLHDHRCLRLAILN
                     ACEGGRADRKDPFAGTAQSLLQQGIPAVIAMQFEITDDAAIAFAHEFYEALSDNYPVD
                     AALTEARKSLFGQGRGLEWGTPVLYLRAPNGRIFDVQRTPESGKQRAEPVQQKVQAVA
                     PPPVQSQPQPQVETQPQPQPHAQTQPRIEEKARTEPEVVPVPPVQRSAQVQPQAQPDT
                     RERVQALYNQAQSAVASENWAGALQLWQAVLALEPNNPQARGGFEYAQKQGQLAAAFE
                     DGKKHYNAGRLQQAIDAFNWVRTTAGSNYKGVDGYISQAQSAIAARQAQARPATPPAH
                     QTGPQPTVSTNQPATQAPHAPTHQSASTPASVTPPQYNSQAATPAAPAKQGGMSVMMI
                     VIIVVIATIVVGFLACAMLGLLVEANNYSY"
     gene            complement(2039..2368)
                     /locus_tag="VFH60_00700"
     CDS             complement(2039..2368)
                     /locus_tag="VFH60_00700"
                     /inference="COORDINATES: protein motif:HMM:NF041216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CU044_2847 family protein"
                     /protein_id="HET6312326.1"
                     /translation="MPKRLVEFQLDGGETVLMEVDDAGPSGGPTYRSAHKQSAVVEQA
                     QVTYEQALDRVRPAAESIVQRMRSLGEPPDEVEVQFGLKMSAEFGAFIASATAEVNYV
                     IRLVWRR"
BASE COUNT          424 a          836 c          833 g          508 t
ORIGIN      
        1 taataagaat agttgttagc ctccaccaat aaccccagca tcgcgcaagc caagaagcct
       61 actactatcg tggcaatcac cacgatgatc actatcatca taacgctcat gccaccctgc
      121 ttggcgggcg cggccggtgt agcggcctgg ctgttgtact gcggcggcgt gacgcttgcg
      181 ggcgtgcttg ccgactggtg ggtcggggca tgcggggcct gtgtcgcagg ttggttcgtg
      241 gacacggtgg gctgcgggcc ggtctgatga gcgggcggtg tggccggtcg agcctgggcc
      301 tggcgagcgg ctatggcgct ttgggcctgg gagatgtagc catcgacgcc cttatagttg
      361 ctgcccgccg tggtgcgcac ccagttgaac gcatctatgg cctgttgcaa acgcccggcg
      421 ttgtagtgct tcttgccgtc ctcgaaggcc gccgccaatt gcccctgttt ctgtgcgtac
      481 tcgaacccgc ccctggcctg cgggttgtta ggctccaacg cgagcacagc ctgccacagt
      541 tggagcgcac ccgcccagtt ctccgaggca acggctgact gcgcctggtt gtacagcgcc
      601 tgcacacgtt cccgcgtgtc gggctgagcc tgcggctgga cctgcgcgct ccgctgcaca
      661 ggcggcacag gtaccacttc cggctcggtt cgggcctttt cctcgatccg cggctgggtc
      721 tgcgcatggg gctgaggttg aggttgggtc tctacctgtg gttgaggttg ggactggacg
      781 ggcggcggtg cgaccgcctg taccttctgc tggaccggct ctgcccgctg cttgccgctc
      841 tcaggagtgc gctgcacgtc gaagatgcgg ccgttgggag cgcgcaggta gagcaccggc
      901 gtaccccatt ccagccccct gccttgcccg aacagcgact tgcgggcctc ggtcagcgcg
      961 gcgtccaccg ggtagttgtc cgacagcgcc tcgtagaact cgtgggcgaa cgcgatggcc
     1021 gcgtcgtccg taatctcgaa ctgcatggcg atcacagccg ggatgccctg ctgcaagagg
     1081 ctctgggcgg tacccgcgaa ggggtctttg cggtcggcgc ggccaccctc gcacgcgttg
     1141 aggatcgcca ggcgcaagca gcggtggtcg tgcagcagca tgccgaggtc ctgcccgctc
     1201 accgcccggc cccgcccgtt atcgtcctcc agtatgagca cgccctgctc ggtctggtgg
     1261 tcgtagccgc catgcccgat gaagtggaag atgtggtact cgccgcgccg cagcttgcgc
     1321 tgcaaggccg ccaggttcgc gtcctccatc gtctcgatct ccaccaaccc gcgcgcctcg
     1381 aggtctgcca gcgccgagtg gaggttcttc gcctcggcct gcgtgtcgag cgcgtcaaaa
     1441 tcgctgtggc tggacaccat catcagcacg cgcaggggcg ggttcacggt gagcggtgcg
     1501 atgcgctcgc gcaactccaa atagcgcacc accggcgtct cgacggagag ggccaggaac
     1561 ttgttgttgg cggggttgta gaggtactcc cagggcaggt cggcaagctc cggggcctcg
     1621 gtcagcgaga ggcggaggcg gaggcgggcg tcctgctgct gggcgtagct gaggtcgcgg
     1681 cgcaggcact cctgggcatc acccttgaac agggtgttga acagcttgcc gccgaactcc
     1741 ttagccgccg ccgtctccac cgtgtcgatg cgccgcacgc tacgcctgct gtagcccgcc
     1801 ttgagcagga agttctcaag ctccatgtcg ctgaacggct tgccgaacgt cacggtgacg
     1861 ttctcgcccg actgcgagtg caggtgcgcc cggtagccgt taccggcgcg ctggatggtg
     1921 atatcgaaat tgaggtagtt gatgtccttc caactgctac tcatgatggt tctccaatgc
     1981 accccggacg aggcatcacg tccttctaca acgccacgct acctcttatg tcaagtcttc
     2041 acctgcgcca caccagccgg attacgtagt tcacctcggc agtggccgaa gcgatgaagg
     2101 ccccgaactc cgcgctcatc ttcaggccga actgcacctc tacttcgtcc ggcggctcgc
     2161 ccagggagcg cattcgctgc acgatgcttt cggcggcggg gcgtacgcgg tccagggcct
     2221 gctcgtaagt tacctgggcc tgctcgacca cggcgctttg cttgtgggcc gacctgtacg
     2281 taggaccccc ggacggcccc gcgtcgtcca cttccatcag cacggtctcg ccaccatcga
     2341 gctgaaactc caccaggcgc ttaggcatgt accctctccc ttacaaatgc aacagggccg
     2401 cacactatgg gtgcagcctc aaccggcccc ttccacagcg cagttaccac gagtatagtg
     2461 ccttggagta agcattgtca atggaagaat tataggagac ggaaccgcca agacgccaag
     2521 aacgcaaagg accacgccaa gagttaagta gtgtggtgtt ctgagcaaag agggccaggg
     2581 tatgacgtga tgtaccctgg t
//
LOCUS       DASRWZ010000051         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1168419, whole genome shotgun sequence.
ACCESSION   DASRWZ010000051 DASRWZ010000000
VERSION     DASRWZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168419"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFH60_00705"
     CDS             complement(<1..339)
                     /locus_tag="VFH60_00705"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET6312327.1"
                     /translation="MTGTENNEPRGRGLSVRDVLFAWGPAILWMGAIFYVSSVNTWTA
                     TPRVPEWQGLRKLAHICEYAVLALLVGRALLGTWAAGGVGVTRGLMLRVWVWGAVISG
                     LYAVSDEVHQG"
     gene            785..1621
                     /locus_tag="VFH60_00710"
     CDS             785..1621
                     /locus_tag="VFH60_00710"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6312328.1"
                     /translation="MNKSAVAALTFPGSPVAGFTLNAWGSVFGPRTGIANNPGRPGGE
                     VPANRTVTMPSPDAGNLPADTVQGVQASQDLAHAGANVNTYAAPAAEPAVLTSAEQDR
                     LLVQAALSGDQLAFGELVTRYQTAVYNMAYRMLGDPTEAEDAAQEVFVRAWNQLHTFQ
                     LDRRFSTWLLSIASHHSIDLLRRRKPQAPLDDVALYVQSDDPEPEELAIRAEQSDMVR
                     QLLNTLPDKYRSVTVLRYYNDLSYDEIARVTGLTESAVKTQLHRARKMLAEELAEVNG
                     RR"
     gene            1703..2428
                     /locus_tag="VFH60_00715"
     CDS             1703..2428
                     /locus_tag="VFH60_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6312329.1"
                     /translation="MSCKSTYDELMSLKLDNLLDPAGERGLQVHLAGCEDCSLTWQAM
                     SQADALLCATATAPVPVPANFHAAVMLKVSQAQVYRPQLAEVDAPVPVPVFVPALGSV
                     LPAPVPQPVRAAAGGLDEDAWQEWPWQEWQRRLSQYLRGAAAVGLSVAGTSGLVIALM
                     LSDVIKVEGPFAGFLGMLRTFLSAVNAWLGSLFTGVGAGTVAGVSLVMGIMLLAAWQL
                     VAAYHRTAEMQHGNIGQLAEAVS"
     gene            2482..>3302
                     /locus_tag="VFH60_00720"
     CDS             2482..>3302
                     /locus_tag="VFH60_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET6312330.1"
                     /translation="MHTQDRTYKKTSRLLLLLSVVAGLTFLATAYSITHAIAHAASLE
                     SQLAQAETSRSAVGEDLHVGAGEVVQGDATVTDGDMTVDGEVRGNVVVVQGNAYIHGK
                     VVGDVSVMGGDARLSAGSSVTGNVLVLAGGKVEREEGASIGGEVSTVDFPLLPTNASV
                     GFPSIPGQPSRSHEQFGGIFDALGRVATLVLVLGLGLLLTVFVLAFALIVPHRLQVSS
                     ATLEAVPGPSVAVGLIVALLMWPVFGIVSMLLTLTIVGIVLVPVLGIIVALALLFG"
BASE COUNT          600 a         1178 c          935 g          589 t
ORIGIN      
        1 cccctggtgc acctcgtcac ttaccgcgta caggccggaa atcaccgccc cccataccca
       61 cacccgcagc atcaagccac gcgtcacccc gaccccgcct gccgcccacg tccccagcaa
      121 cgccctcccc accagcaaag ccagaacggc gtactcgcag atatgggcta acttacgcag
      181 tccctgccac tcaggcactc ttggcgtcgc cgtccaggtg ttcacggaag acacatagaa
      241 aatcgccccc atccagagga tggcaggccc ccatgcaaag aggacatccc gtacagacag
      301 cccgcgcccg cgcggctcgt tgttctcggt gccggtcacg ctcatttgtc catagaggat
      361 agcagagggg taggggagtg tcaagcacat aggtagtcag cacccgggag tacgtattcc
      421 cggtatggag caggtcgttt gtcatgtggg gctgccctgt ggctgaagcc acgggcttac
      481 aagaggcaaa gtcccctacg gggactaagg aacatcatgt agtctccgta ggagacttcg
      541 tctctggtaa gcctgccaat tcattggcag ggcaccccca cagggcatcc cccacaaggc
      601 ctcgacatag catagcgtag gcccattacc attgccggac cgcaggtacc acatacatgt
      661 agccgccacc cctaaacccc tgctctaatt gccctgccac aaaccgaatc gaaactgccc
      721 aaatccctgc aacatcctca agcctccttg cgtatatagg gtgtgcaaga ggaggcacgt
      781 tttgatgaat aaaagcgctg tggctgcttt gactttcccc ggttctccgg tcgcgggttt
      841 tacactcaac gcttggggtt cggtgttcgg ccctcgtacc ggcatcgcca acaaccccgg
      901 tcgtcccggt ggcgaagtcc cggccaacag gacagttacc atgccgtcac ccgatgccgg
      961 caacctgccc gccgacacgg tgcagggcgt ccaggcctct caagacctgg cgcacgcggg
     1021 cgctaacgtg aacacatacg cggccccggc cgccgagcct gccgtgctga cttcagccga
     1081 gcaggaccgg ctcctcgtgc aagccgccct ctccggcgac cagctagctt tcggcgagct
     1141 tgtcacccgc taccagactg ccgtgtacaa catggcctat cgcatgctgg gcgaccccac
     1201 cgaggccgag gatgccgccc aggaagtgtt cgtccgcgcc tggaaccagc tacatacgtt
     1261 ccagctagac cgccgcttct ccacctggct gctctccatc gcctcccacc atagcatcga
     1321 cctgctccgc cgccgcaagc cccaggcccc cctcgacgac gtggcactct acgtgcagag
     1381 cgacgacccg gagccggaag agcttgcgat cagggccgag cagagcgata tggtcaggca
     1441 gttgctcaac accctgcccg acaagtaccg ctccgtcact gtcctgcgct actacaacga
     1501 cctgtcctat gacgagatcg cccgcgtcac cggcctaacc gagagcgcgg tcaagacgca
     1561 gcttcacagg gccaggaaga tgctcgcgga agaattggcc gaagtaaacg ggagaaggta
     1621 atattatgga ctacgcttac gcaggctatc cttcatactt catagaccgc gcgccatccc
     1681 tgtccgaagg gggtacgctc tgatgtcgtg caagtccacc tacgacgaat tgatgtccct
     1741 caagctggac aacctgctcg accccgccgg tgagcgcggc ttgcaggtcc accttgccgg
     1801 gtgcgaagat tgctccctca cctggcaggc tatgtcccag gccgacgcgc tcctctgcgc
     1861 caccgccacg gcccccgtac ccgtgcccgc caacttccat gcagcggtca tgctcaaggt
     1921 ttcccaggcc caggtatacc ggccccaact agccgaggtt gacgccccgg tgcccgtgcc
     1981 cgtatttgtc cccgccctcg gttccgtgct ccccgcccct gtccctcaac ccgtacgagc
     2041 ggctgcgggc ggcctggacg aggacgcctg gcaggagtgg ccgtggcagg agtggcagcg
     2101 ccgcttgagc cagtacctgc ggggcgcggc cgccgtgggc ctctccgtcg ccggtacctc
     2161 cggcctggtg atcgccctca tgctgtccga cgtcatcaaa gtcgaggggc cgttcgccgg
     2221 tttcctcggc atgcttcgca ccttcctgtc cgccgtgaac gcctggcttg gctccctctt
     2281 cacgggcgtc ggcgcgggca ccgtggcagg cgtctccctg gtaatgggca ttatgctgct
     2341 ggccgcctgg cagttagtag ctgcctacca tcgcaccgcc gagatgcagc acggcaacat
     2401 aggccagctt gcggaagccg tgagctgacc ccaacccgta tttagaggta aacagacgag
     2461 attttggcta gagaacacgt catgcatact caagaccgca catacaagaa gacctccagg
     2521 ctgctcctgc tcctctccgt agtagccggg cttaccttcc tcgcgaccgc atactccatc
     2581 acccatgcca tcgcccacgc cgcctccctc gaaagccagc tagcccaggc tgagacgtcc
     2641 agaagcgccg tgggcgaaga cctgcacgtc ggcgcgggcg aagtagtcca gggcgacgct
     2701 accgtcaccg acggtgacat gacggtggac ggcgaagtgc gcggcaacgt cgtggtcgtc
     2761 cagggcaacg cctacatcca cggtaaggtg gttggcgacg tgtccgtcat gggcggtgat
     2821 gccaggctta gcgcgggtag cagcgtcacc ggcaacgtgc tcgtgctcgc gggcggcaag
     2881 gtcgagcgcg aagagggggc cagcataggc ggcgaggtca gcactgtgga cttccccctg
     2941 ctgcccacca acgcctcggt cggctttccc tccatccccg gccagccctc gcgctcccac
     3001 gagcagttcg gcggcatatt cgacgccctg ggcagggtag ccaccctcgt cctggtgctc
     3061 ggcctgggct tgctcctcac cgtgttcgtg ctcgccttcg ccctgatagt gccccaccgc
     3121 ctccaggtgt cgagcgccac gctcgaagcc gtgcccggcc cctccgtcgc cgtcggcctc
     3181 atcgtggccc tgctcatgtg gcccgtgttc ggcatcgtaa gcatgctcct aaccctcacc
     3241 atcgtgggca tagtgctcgt gcccgtgctc ggcatcatcg tggccctcgc cctgctcttc
     3301 gg
//
LOCUS       DASRWZ010000052         1902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1305733, whole genome shotgun sequence.
ACCESSION   DASRWZ010000052 DASRWZ010000000
VERSION     DASRWZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305733"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFH60_00725"
     CDS             <1..584
                     /locus_tag="VFH60_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET6312331.1"
                     /translation="IQNARTLAQQHGFLVLELDRIDSVEWAEGVLAQQDPPENAILRQ
                     TSQIAVRVSRGPPPFSLPNLTNTDPNEARGVLESTGLRVVIAYEGSQTIPKGIVARTN
                     PPADTSVKSGQQVTIYISLGKSQIVPDLRGTENLELAQQRLEALGLSVGTVTEEDDPN
                     ESVPPGAVLRTEPNAGTEIEENGVVNIVLRRRQ"
     gene            730..1446
                     /locus_tag="VFH60_00730"
     CDS             730..1446
                     /locus_tag="VFH60_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6312332.1"
                     /translation="MIEAHEVVPGLWLFRGDASGRNFTVLLGDRGAAVVDPGLPDAVN
                     RFVAEMSAELEAVVLTAGVGPSAPAPADEEVPSAWPTLPLLTPEMFVGRTPLPISIPA
                     WDALPLAGAPPSMALYETRQRALLAGDLLPEPSVGVPDLRAGAEPYLENLERIEALDP
                     RLVVPSRGEPATGRRAIKARIENDRNYVYSLTRHVATSAASGVPLDRLLQVATDLYDD
                     FPHLQAHLANMRHIWDELRS"
BASE COUNT          396 a          683 c          500 g          323 t
ORIGIN      
        1 acatacagaa cgccaggacc ctcgcccaac aacacggctt cctcgtgttg gagctagacc
       61 gcatcgacag cgtggagtgg gccgaaggtg tgctagccca acaggacccg cccgagaacg
      121 ccatcctcag gcagacgagc cagatagccg tccgcgtcag ccgcggcccg cctcccttca
      181 gcctgcccaa cctcaccaac accgatccca acgaagcgcg cggcgtgctg gaaagcaccg
      241 gcctgcgggt ggtcatcgcc tacgagggca gccagaccat ccccaagggc atagtcgcca
      301 ggacgaatcc gcccgccgac accagcgtca aatcgggcca gcaggtgacc atctacataa
      361 gcctgggcaa gagccagatc gtgcccgacc tgcgaggcac cgaaaacctc gagctcgctc
      421 aacagcgcct cgaagccctc ggtctctccg taggtaccgt caccgaggaa gacgatccca
      481 acgagagcgt cccgcccggt gccgtcctgc gcaccgaacc aaacgcgggc accgaaatcg
      541 aagagaacgg cgtggtcaac atagtcctcc gccggagaca gtagcgagtg ccgagtaccg
      601 agtaccgagt agcaagtaac accagtgcta atcatattct tctttgcgcg ttctttgcgt
      661 ctttgcgact ttgcgtttcc gtctttatac tcggcactcg gcactcggca ctcctgaatg
      721 gagcaaagct tgatcgaagc gcatgaggtg gtgccggggc tgtggctctt ccggggcgat
      781 gcttccgggc gcaacttcac cgtgctgctg ggtgacagag gcgcggcggt ggtagacccc
      841 ggcctgcccg acgccgtcaa ccgcttcgtg gccgagatga gcgccgagtt ggaggccgtg
      901 gtcctgaccg ccggcgtagg gccatccgcc cctgcgcccg ccgacgaaga agtcccttca
      961 gcctggccca ctctgcctct cctcaccccc gaaatgttcg tgggccgcac gcccttaccc
     1021 atctccatac ccgcctggga cgccctgccc ctcgcgggcg ctcctcctag catggccctc
     1081 tacgaaacgc ggcaacgcgc gctcctcgcc ggcgacctgc tgcccgaacc cagcgttggc
     1141 gtgccggacc ttcgagccgg ggccgaaccc tacctggaaa acctggaacg tatcgaggcc
     1201 ctcgaccccc ggctggtggt cccctccagg ggcgaacccg ccacgggcag gcgtgctatc
     1261 aaagctcgca tcgaaaacga ccgcaactac gtctactcac tcacccgcca cgtagccacc
     1321 tccgccgcct ccggcgtgcc cctcgaccgc ctcctccagg tcgccaccga cctctacgat
     1381 gacttccccc acctccaggc ccacctcgca aacatgcgcc acatctggga cgagctgcga
     1441 agctaaaacg tgcaaggtgc tacggccacg gtctaaactg cgtgactgcg acgctatgga
     1501 gatggctgaa cccagagtgc ctctgtcatc ctgagtgcag cgaaggatca gccgcccgtg
     1561 tttgatagcc ccttctacag cacccgaagc tcccctttgt cattctgagc gtagcgaaga
     1621 atctcgtcct ccagcaccca gacagccctt ttgggtagca ctcacctgta agaaaagtgt
     1681 aacccccagg aagggaacga gattcttcac tgcgttcaca atgacaaacg ggaacagctc
     1741 ccttttagcc caacagcaca acattcacgt attgccagaa gcaagcccca taggtctcac
     1801 ccccgacgat ttagaaccct atttctccag gaactcgttc gtaaacatct cctcgacatc
     1861 caccttcttc ttgatgatcc cgaaatcgaa cagcgcatcc tg
//
LOCUS       DASRWZ010000053         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_72428,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000053 DASRWZ010000000
VERSION     DASRWZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72428"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2421
                     /gene="lon"
                     /locus_tag="VFH60_00735"
     CDS             <1..2421
                     /gene="lon"
                     /locus_tag="VFH60_00735"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6312333.1"
                     /translation="PDGTISLLVQGQRRVRLLRFLRMDPYMRVMGEGIEDQSEKTSTT
                     EALMRAVLALFEKVVKLSRNLSDESYVAAMNVDEPGWLADLIASTITLEQEDRQKLLE
                     TIDPIERLQTLSVMLAKELDVLDLENTIQDRVQNEVDKSQREFYLREQMKAIAQELQD
                     YDPQTREANDLRDRIEAAGMPEDVHKKAIEELERMQAMPMGMPEVGIIRTYIDWLLAL
                     PWHNQTPDHLDLKAAAKVLDANHYGLGKVKERIIEYMAVRKLAQGKMRSPILCFVGPP
                     GVGKTSLGRSIAEALGRRFVRVSLGGIRDEAEIRGHRRTYIGALPGRIIQTMKTAGTV
                     NPVFMLDEIDKVGADFRGDPSAALLEVLDPEQNNAFSDHYLDVPYNLSKVMFIMTANL
                     LDPVPPALLDRMEVIELPGYIEDEKYHIARQFLAPRQIEEHGLTPAHIKVTDGAIRRI
                     IREYTHEAGVRNLEREIGAICRKVAKRVAEAMPPDEVPVVGEQVSGDIPHSTLSNPHL
                     TEHPTEPLPEDALLSQYQAEIDAIERIEAAQEQDAVSDMAPAPVEAEPAGKRLRVRTI
                     RERDLEHYLGPSRFSYGMAEEEDEAGLATGVYWTPMGGDIISVEVTLMEGKGNLLLTG
                     QLGDVMKESAHAALSYARTRARQLGIDPARFEKTDIHVHVPAGAIPKDGPSAGVTLGT
                     ALVSALTARKVRRDVAMTGEITLRGKVLPIGGLKEKVLAAHRAGISTFILPRKNEKDL
                     DEIPQKVRRELEMVAVEDLDAVLKVALRPAPNLSDSKPEPVSEPPPRPAAPTPTRQAT
                     RTTGVHPS"
     gene            2514..>3475
                     /locus_tag="VFH60_00740"
     CDS             2514..>3475
                     /locus_tag="VFH60_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312334.1"
                     /translation="MTWVWIVYIAATLLISVLVGLHLGRFREAYTEMTGMIAGMAMGM
                     LNGFLLGYAAAAITGSMFWGNLFGVLMGLSMGAYFGRAGSLMGVMDGAMGGVMGGSMG
                     AMLMVMLVWPPHIMWTAALLAAIYLAGMFGLVLLIERSAPEHAALHRLMPIFTRAVAI
                     EALEAQDQASSTPHSLPIPDYYALLSIHPDANDGEITQAYLKQLATTDEASVEQLEIA
                     LATLTHPSRREAYDRLLATSKAEATSESPGPEISTYQALQMQPAPRARREAPISWVGV
                     VAAIAIVVILAGWWLANQGTRTAASNFANPLNLPADFVQKLEAKA"
BASE COUNT          715 a         1185 c         1010 g          565 t
ORIGIN      
        1 cccgatggca ccatctccct cctggtgcag ggccagaggc gcgtcaggct gctgcgcttc
       61 ctgcgtatgg acccgtatat gcgcgtcatg ggcgagggca tcgaagacca gtccgagaag
      121 acctctacca ccgaggcgct gatgcgggcg gtgctggccc tcttcgagaa ggtggtcaag
      181 ctgtcgcgca acctctccga cgaatcctat gttgccgcca tgaacgtgga cgagccgggc
      241 tggctggccg acctcatcgc ctccaccatc acccttgagc aggaggaccg ccagaagctc
      301 ctcgaaacca ttgaccccat agagcgcctc cagaccctca gcgtcatgct cgccaaagag
      361 cttgacgtgc tcgacctgga aaacaccatc caggaccgag tgcagaacga ggtggacaag
      421 agccagcgcg agttctacct gcgcgagcag atgaaggcca tagcccagga gttgcaggac
      481 tacgacccgc agacccgtga ggccaacgac ctgcgggacc gcatcgaagc cgctggtatg
      541 cccgaagacg tacacaagaa ggctatcgaa gagcttgagc gcatgcaggc tatgccgatg
      601 ggcatgccgg aggtcggcat catccgcacc tatattgact ggctgctcgc cctcccctgg
      661 cacaaccaga cccccgacca cctcgatttg aaggcggcgg cgaaggtgct ggacgccaac
      721 cactacggct taggcaaagt gaaagagcgc atcatagagt acatggccgt gcgcaagctg
      781 gcgcagggca agatgcgctc gcccatcctc tgcttcgtcg gccctcccgg cgtaggcaag
      841 acctccctgg gccgctccat cgcggaggca ttgggccgca ggttcgtgcg cgtgtcgctt
      901 ggcggcatcc gcgacgaggc agagatacgc ggccaccgcc gcacctacat aggcgcgctc
      961 ccaggccgca tcatccaaac catgaagacc gcaggcaccg tcaaccccgt atttatgctg
     1021 gacgagatag acaaagtggg cgcggacttc aggggcgacc cctccgccgc gctccttgag
     1081 gtgctcgacc cggagcagaa caacgccttc tccgaccact acctcgacgt gccctacaac
     1141 ctgtcgaagg tgatgttcat catgaccgcc aacctgctcg accctgtgcc gcccgccctc
     1201 ctggaccgca tggaagtgat agagcttccg ggctacatcg aagacgagaa gtaccacatc
     1261 gcccgccagt tcctcgcgcc gcgccagata gaggagcacg gcctcactcc cgcccatatc
     1321 aaggtgacgg acggcgctat acggcgcatc atccgcgagt acacgcacga agcaggcgtc
     1381 cgcaaccttg agcgtgagat aggcgctatc tgccgcaagg tcgccaagcg tgtagccgaa
     1441 gcgatgccgc cggacgaggt gccggtggtc ggtgagcagg tatcaggcga tattccgcat
     1501 tccacactca gcaatccgca tttgaccgag catccgaccg agcctctgcc ggaggacgcc
     1561 cttctgagcc aataccaggc cgagattgac gctatagaga ggatagaggc cgcgcaggag
     1621 caggacgccg tctcagatat ggcacccgct cccgtcgagg ctgagcctgc cggcaagcgc
     1681 ctccgcgttc gcacgatacg tgagcgcgat cttgagcact atttaggccc gtcccgcttc
     1741 tcctacggca tggccgaaga ggaagacgag gccggcctgg ctaccggcgt ctactggacg
     1801 cctatgggcg gcgacatcat cagcgtggaa gtcactctga tggagggcaa gggcaacctg
     1861 ctcctcaccg ggcagttggg cgacgtgatg aaggagtcgg cccacgccgc gctctcctat
     1921 gcccgcaccc gcgcccgcca gcttggcata gaccccgccc gcttcgagaa gaccgacatc
     1981 cacgtccacg tccctgcggg cgctatcccc aaggacggcc cttcggcggg cgtcaccctg
     2041 ggcacggccc tggtatcggc cctcaccgcc cgcaaagtgc gccgcgacgt agcgatgacc
     2101 ggcgagatca ccctgcgcgg caaggtgctc cccattggtg gcctcaaaga gaaggtgctg
     2161 gcagcccacc gcgccggtat ctctaccttc atcttgccgc gcaagaacga gaaagacctg
     2221 gacgagatac cccagaaagt gcgccgcgag ttggagatgg tcgcggtgga agacctagat
     2281 gccgtgctca aagtagccct gcgcccggcc cccaacctga gcgacagcaa gcctgagccg
     2341 gtcagcgaac cccctccacg ccccgcagcc cccactccca cccgccaggc cacgcgcaca
     2401 acaggcgtcc accccagcta gtgaccagtg accagtgacc agttagcact cagcactcgg
     2461 tactcatcac tggtcactga cccctgaccc ctgacccctc agaaaggaca tatatgacct
     2521 gggtttggat agtttacatc gcagccacgc tactcatcag cgtgctggtt gggctgcacc
     2581 tggggcggtt ccgtgaagcg tacacggaga tgaccggcat gattgccggc atggcgatgg
     2641 gcatgctgaa cggctttctg ctcggttacg ccgccgccgc gataacaggt agtatgttct
     2701 ggggcaacct cttcggcgtg ctcatgggcc tctcgatggg cgcgtatttt gggcgcgcag
     2761 gcagcctcat gggcgtcatg gacggcgcga tgggcggcgt catgggcggc tcgatgggcg
     2821 ctatgctcat ggtaatgctg gtgtggcccc cgcacatcat gtggaccgcc gcgctcctgg
     2881 cggccatcta cctggcaggc atgttcggcc ttgtcctcct aatcgagcga agcgcacctg
     2941 agcacgccgc cctccaccgc ctcatgccca tattcacccg cgccgtagca atcgaagccc
     3001 ttgaagcgca ggaccaggct tcatctaccc cgcactccct gcctataccc gactattacg
     3061 ctctactcag catccacccc gacgccaacg atggggagat cacccaggca tacctcaagc
     3121 agcttgccac cacagacgaa gcaagcgtgg agcaactgga gatagcacta gccacgctca
     3181 cgcacccctc acgacgcgag gcatacgacc gccttctcgc aacaagcaag gccgaagcca
     3241 cttcagagtc gcctggcccc gaaatctcta cataccaggc cctacagatg cagcctgccc
     3301 caagagcacg ccgcgaagcc ccgatctcgt gggtaggcgt ggttgcggct atcgctatcg
     3361 tagttattct agccggatgg tggctggcta accagggcac gcgcacagcc gcgagcaact
     3421 tcgccaaccc actaaacctg cctgccgact ttgtgcagaa gctggaagct aaagc
//
LOCUS       DASRWZ010000054         4826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1305899, whole genome shotgun sequence.
ACCESSION   DASRWZ010000054 DASRWZ010000000
VERSION     DASRWZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305899"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VFH60_00745"
     CDS             complement(<1..861)
                     /locus_tag="VFH60_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6312335.1"
                     /translation="MADAKEVTVIIDGVPVKVPANTYVWDACRIVGQEIPNFCYIPGL
                     RAFGACRMCVVEVSGRRGFDLVTSCSTPVTDGMEVRTINERIWQQRNMIMEALDTDHP
                     VDCPICEANGDCRLQDYGYEYGVTGNDTRRPKVVRPAERLSPAIDIDRDRCVMCGRCV
                     RECDEVIGATALAIVERGLESLIDAPFGKSLLETPCVSCGQCVELCPVGALSSRLYSS
                     RMTLEHHWWQRKTETTCNSCSVGCTIKLGSTRNQIWEVRSDDALGLNDGRTCVKGRFG
                     QDYINSNSRLS"
     gene            complement(893..1234)
                     /locus_tag="VFH60_00750"
     CDS             complement(893..1234)
                     /locus_tag="VFH60_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HET6312336.1"
                     /translation="MARPYPTWCCWQTGHSRRSNKKEQSQTVTAVYENGVFRLTSELE
                     VPLQEGQRVRLTAEQLPPPDEMMELFANFYDGFSKEEIAEIEKIMLDRSNFFPERDWS
                     WLDDEDAQQEG"
     gene            complement(1626..1859)
                     /locus_tag="VFH60_00755"
     CDS             complement(1626..1859)
                     /locus_tag="VFH60_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312337.1"
                     /translation="MSRLIALDDELYGKLEQVASRHGFDNVENVVAEMSKEESAYRRR
                     EAVRQIKDVYAYMKTKYGRMENSVTLIREDRAR"
     gene            complement(2069..3982)
                     /locus_tag="VFH60_00760"
     CDS             complement(2069..3982)
                     /locus_tag="VFH60_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET6312338.1"
                     /translation="MSEVAHYYPIDVRQRTQVSKEQFEARQQAARRRWQATMTQAEVP
                     VIIVPWGSSSVAHGVVATFEEIRQWLSENEVRAIVRRAGPMGTHYLEPQVDIILPGMP
                     RISYAHITADKVPDLLHSVLELNDLRPDLAVCIYGTEEWDGAELGVRGIPVAKELDFW
                     KLQQRMVGRNLGYIDPESIDDYIARGGYEALKRALSGISPDDLIQMISDSGLRGRGGA
                     GFPTGTKWSGGRKARKTPKYVICNSHEGEPNVFKDRVLHEGDPHQIVEGLILQAYAVG
                     TPWAYIYVGDEYPLAIHRTKIAVQQAYAAGLLGENIMGSGFSCHMDVQMGGGAYISGE
                     ASALMFGIEGKRAMPRTKPPRSVEAGLFNLPTVVNNTETISCVPHIVVNGPQWFSAIG
                     TEKSKGTKLFTLMGSIKTIGVYELPLGRTMRELVEVCGGGTLDGHPFKAIQTGGPSGG
                     CMPEQYLDVIMDFDSLDQYGGTMGSGGYVVYDDRTCIVDMSRYFGRFNRYQSCGKCVP
                     CRIGTKNLLHMVDRIALGGGKPEDIPIMMKWSDHVVEMSLCGLGQTAPLPLLGGLRYF
                     LEEFEEHINQKYCRTGTCPIHAITVEERFLPARIGERYRQQLSAIPYGNQYTKGNGAG
                     SAPASTQGAAVRR"
     gene            complement(4069..>4826)
                     /locus_tag="VFH60_00765"
     CDS             complement(4069..>4826)
                     /locus_tag="VFH60_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET6312339.1"
                     /translation="PLADSTAISPGRAIPPDQISAIVERYKHVDPLEAMIPVLQEMQV
                     RFGYITQDAAEQMAVELGLTAAEVYGVITFYSFFRYTPRGGHVIMSCEGTSCYVRGAG
                     QIREAIENRLDVGPGDTSADGFFTFEPQSICLGACDLGPLVDIEGTFYTFVTPEKMDA
                     ILTQWYEAGDETPVGDNLTGGHLPHYGDNHGFGPTADDLYAGYEVAQFMPAGVGGVAS
                     NTSPLPGVEEAIQNMPPTPGTGGPSGSGSASQS"
BASE COUNT          835 a         1568 c         1394 g         1029 t
ORIGIN      
        1 gctcaggcgg ctgttcgagt tgatgtagtc ctgcccgaag cggcccttca cgcaggtgcg
       61 gccgtcgttg aggcccaagg cgtcgtccga gcgcacttcc catatctggt tgcgggtaga
      121 acctagcttg atcgtgcagc ccaccgagca ggagttgcag gtcgtctcgg tcttgcgctg
      181 ccaccagtgg tgctccagcg tcatcctgct gctgtagagg cggctggaga gggcacccac
      241 cgggcaaagc tccacgcact gcccgcacga cacgcagggc gtctcaagga gcgacttgcc
      301 gaacggcgcg tcaatcagcg attcgagacc gcgctccaca atggcgaggg cggtggcacc
      361 gataacctcg tcgcactcgc gcacgcaccg cccgcacatc acgcagcggt cgcggtcgat
      421 gtcaatcgcg ggggagaggc gttcggcggg gcgcaccacc ttggggcggc gggtgtcgtt
      481 gccggtcacg ccgtactcgt agccgtagtc ctgcaagcgg cagtcgccat tggcctcgca
      541 gatggggcag tctaccgggt ggtcggtatc gagcgcctcc atgatcatgt tgcgctgctg
      601 ccagatgcgc tcgttgatcg tgcgcacctc catgccgtcg gtcacggggg tggagcagga
      661 ggttacgagg tcgaagcctc tgcgccccga cacctcgacc acgcacatgc ggcaagcgcc
      721 gaacgcccgc aggcccggta tgtagcagaa gttggggatc tcctgcccca ctatgcggca
      781 ggcgtcccac acgtatgtgt tggcgggcac cttcaccggc accccgtcta tgatgaccgt
      841 tacttccttg gcgtctgcca ttacctgctc ctaggtatat ttgtgaacgt aactagccct
      901 cttgttgcgc gtcttcgtca tctagccaac tccagtccct ttcggggaag aagttgctgc
      961 ggtccagcat tatcttctcg atctcggcta tctcctcttt cgagaaacca tcgtagaagt
     1021 tggcaaacaa ctccatcatc tcgtcaggtg gtggtaactg ttccgctgtg agcctgaccc
     1081 gctgaccctc ctggagaggc acctcaagct cgctcgtcag ccggaatacg ccattttcgt
     1141 atactgcagt taccgtttgt gactgttcct tcttgttgct ccttcggcta tgaccagttt
     1201 gccagcagca ccaagttggg tagggacgcg ccaactgagt tgaataaccg ctcgtccgct
     1261 gttaccagcg ggcactgttc tcgtatgctc agtgcaagat aaatcgagtc atacatactc
     1321 tgcttgtaac tgatggctat tctacatgca tccaggaaca acgtgcgagt aagtgccacc
     1381 tctgatccaa tgtcagccgc gaagaaggta ttagcaacta tctgaccgtc tgaaggtatc
     1441 aatgcacctc gcgttacttt cttccagatg atgttgccaa cctctgcgta tatcagttca
     1501 ggcacgatta atatgtaatc accagcccca atcccgtcga gtagcacttg agcctagtcg
     1561 ctgtcatcct cgtccacgaa ccacttgatg gctacgctgc tatcaacgac aagcttgctc
     1621 atctactacc ttgctcggtc ctctctaatc agggttacgc tgttctccat tcgcccatat
     1681 ttggttttca tgtaagcata tacatctttt atctgacgaa cagcctcacg cctgcgatat
     1741 gcactttctt ccttgctcat ctctgctacc acgttttcaa cattatcaaa gccgtggcga
     1801 gaggcgacct gttctaactt gccgtacaac tcatcatcaa gggctatcaa cctgctcatt
     1861 gcttgcttcc tctgtctgaa ccgcactctt cccggcttct cggtccttgc tttctaaccg
     1921 ctgcaatccg tcttgcaccc atcgttggaa aagctgctcg attggaactc cgctggaacg
     1981 agcctcttgt tccaatctgg agtatagatc atcaggtata gcgacggtcc ggctcataag
     2041 ggtaactctc acggttctag cttcgacctt accgcctcac cgccgctccc tgcgtgctag
     2101 ccggggccga ccccgcgccg ttgcccttgg tgtactggtt gccgtagggg atggcgctta
     2161 gctgctggcg gtagcgctcc ccgatgcgcg caggcaagaa ccgttcctcc accgtgatgg
     2221 cgtggatggg gcacgtcccg gtgcggcagt acttctggtt gatgtgctcc tcgaactcct
     2281 ccaggaagta gcgcaggccc cccagcaggg gtagcggggc cgtctggccg aggccgcaca
     2341 gcgacatctc aaccacgtgg tcgctccact tcatcataat cgggatgtcc tccggcttgc
     2401 cgccgcccaa cgctatgcgg tctaccatgt gaagcaggtt cttggtgccg atgcggcacg
     2461 gcacgcactt gccgcacgac tggtagcggt tgaaccggcc aaagtagcgg ctcatgtcca
     2521 cgatgcaggt gcggtcgtcg tacaccacgt agccccccga ccccatcgtg ccgccgtact
     2581 ggtcgagcga gtcgaagtcc atgatcacgt cgaggtactg ttcgggcatg cagccgcccg
     2641 aaggcccgcc cgtctgtatc gccttgaagg ggtggccgtc cagcgtgccc ccgccgcaca
     2701 cctccacaag ctcgcgcatg gtgcggccca aaggcagttc gtacacgcca atcgtcttga
     2761 tcgaacccat cagcgtgaac agcttggtgc ccttgctctt ctcggtgccg atagcggaga
     2821 accactgcgg cccgttcacc acgatatggg gcacgcagct aatcgtctcg gtgttgttca
     2881 ccaccgtcgg caaattgaac aggcccgcct cgaccgaccg tggcggcttg gtgcggggca
     2941 tggcgcgctt gccctcgatg ccgaacatca gggccgaagc ctcgccggag atgtacgccc
     3001 caccgcccat ctgcacgtcc atgtggcacg agaagcccga ccccatgatg ttctcgccca
     3061 gcaagcccgc cgcgtacgcc tgttgcaccg ctatctttgt gcggtgtatc gccagcgggt
     3121 actcgtcgcc cacgtagatg taggcccagg gggtgcccac cgcgtacgcc tggaggataa
     3181 gcccctccac tatctggtgc gggtcgcctt cgtgcaggac gcggtccttg aacacgttag
     3241 gctcgccctc gtgcgagttg cagatgacgt acttgggcgt cttgcgggcc ttgcgcccac
     3301 ccgaccactt ggtgccggtc gggaaccccg cgcctcccct accccgcagg ccggagtccg
     3361 agatcatctg gatcaggtcg tcggggctga tgcccgacag cgcccgcttg agggcctcgt
     3421 agccgccgcg tgcaatatag tcgtcaatag actccgggtc gatgtagccg aggttgcggc
     3481 ccaccatgcg ctgctggagc ttccagaaat caagctcctt ggccactggg atgccgcgca
     3541 cgcctagctc ggccccgtcc cactcctcgg tgccgtagat gcataccgcc aaatcggggc
     3601 gcaggtcgtt caattcaagc acgctgtgga gcaggtcggg caccttgtcg gcggtgatgt
     3661 gagcgtagga gatgcggggc atgccgggca ggatgatgtc cacctgcggc tccaggtaat
     3721 gcgttcccat aggccccgcc cggcgcacta tcgcccggac ttcgttttcg gagagccact
     3781 ggcgtatctc ctcgaacgtg gcgaccacac cgtgagccac cgacgacgac ccccacggca
     3841 cgattatcac cggcacctcc gcctgggtca tcgtggcctg ccaccgccgc cgcgccgcct
     3901 gctgccgcgc ctcgaactgc tccttgctga cctgcgtgcg ctgcctgaca tctatggggt
     3961 agtagtgtgc tacttcgctc atatttgctc cgttgctgaa acgtaacgcg taacgcgtaa
     4021 aacgtaactc gtaattagca gatgtactct aggtttacgc gttactgcct agctctggct
     4081 tgccgaaccg ctgccgcttg gcccgcccgt gccgggggtg gggggcatgt tctgtatggc
     4141 ttcctctacg cccggcaggg gcgaggtgtt ggaggcgacg ccgcctaccc ctgcgggcat
     4201 gaactgtgcc acctcgtaac ccgcgtacag gtcgtctgcc gtggggccga agccatgatt
     4261 gtcgccgtag tgcggcaggt gcccgccggt caggttgtcg cccacaggtg tctcgtcgcc
     4321 cgcttcgtac cactgggtca ggatggcgtc catcttctcc ggcgtcacaa acgtatagaa
     4381 ggtgccctcg atgtccacga gggggccgag gtcgcacgcc cccaggcaga tcgattgcgg
     4441 ctcgaacgtg aagaagccgt ccgcgctggt gtcgcctggg ccaacgtcga ggcggttctc
     4501 tatcgcctcg cgtatctgcc ccgcgccccg cacgtagcag ctggtgccct cgcacgacat
     4561 gatgacgtgc ccgccccggg gcgtgtaccg gaagaacgag tagaaggtaa tcacgccgta
     4621 tacttcggcg gcagtcagcc ccaactcgac cgccatctgc tcggcggcgt cctgggtgat
     4681 gtagccgaac ctgacctgca tctcctgcaa caccgggatc atcgcctcga gcgggtccac
     4741 gtgcttgtag cgttccacga tggcgctaat ctggtccggc ggtatggcgc ggccggggct
     4801 gatcgcggtg ctgtccgcca gcggct
//
LOCUS       DASRWZ010000055         4442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_620614, whole genome shotgun sequence.
ACCESSION   DASRWZ010000055 DASRWZ010000000
VERSION     DASRWZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620614"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            538..1851
                     /locus_tag="VFH60_00770"
     CDS             538..1851
                     /locus_tag="VFH60_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6312340.1"
                     /translation="MKGKQSGQTDASGYNRRDFLVNASKLAGAMLAFPILAKAPGAAA
                     QSTGAGSILSSVSSRATERRTVGVLLPQSTAYPKLGANFLAGLDLYLNQSGRKGVSVV
                     SAAVGACCGQAKQEARAMLKSGKVDLMVGLVNPSVADTLVPVLEASNTAFIASSLGEN
                     MVRADEHSSNIFHHTMGMWQANWALGKWAATALGRTAVMASSYYDSGYDTLYAFRMGF
                     EAGGGRVHSTHVTHRPNSDVGLASVMSAIKAAKPNVVFASYYGQTAVDLLNAYARAGL
                     SRSVPLVGTAFLVDQAVVNSVGSAAAGVTTSLPWAASLNTAENQAFITAYQIKTGKSP
                     DAFAVLGYETGGLVASAIDRAAGGNLRDALAGASVVGPRGAIAMNATTQSTSAPHYLQ
                     QAQRQGINVRNVVLADLAAAAELDGQVAALRQTTRTGWISTYLCG"
     gene            2042..2221
                     /locus_tag="VFH60_00775"
     CDS             2042..2221
                     /locus_tag="VFH60_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312341.1"
                     /translation="MEPDGLDCGGTFVEFSWHSFETDGEWDAIAPKAEGAELVGVAGD
                     RTETDDRVAEPAKER"
     gene            2414..2971
                     /locus_tag="VFH60_00780"
     CDS             2414..2971
                     /locus_tag="VFH60_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HET6312342.1"
                     /translation="MQGHQAAHDVAREPSTPSPRRLAFSATVHCLTGCGIGEVLGLVI
                     GSALGWHDLPSIALAVVLAFIFGYGLTLRPLLAGGLRLAEALRLAFAADTLSIAVMEI
                     VDNAIILLIPGAMDAGLTSLLFWGSLAVALAVAFVVAFPVNLWLIRQGRGHAVVHGKH
                     GGHGGHGGHGETVGHTGHDGQPHAH"
     gene            3283..>4442
                     /locus_tag="VFH60_00785"
     CDS             3283..>4442
                     /locus_tag="VFH60_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET6312343.1"
                     /translation="MGKLFLVVCSVLAVAASLHVSASYSAAPIFAEDVQVRVADYSYT
                     PAALAVEAGTTVTWTNTGKDIHNIAIDRGPEAFVSVVLTPGKTARFVFTTPGRYHYFC
                     EWHANMQGDVDVRGPNGSNVAPVTPAARTFAETGKMLRGKFLQYWDAHGGLAQQGFPI
                     SEEMVEKSDTDGKFYTVQYLERAVFELHPDNQPPNDVLLSLLGNFLYKQKYPNGAPGQ
                     QPNTSAGSVLFQETGKRVGGRFLQYWQESGGLPQQGFPISDEFMEKSQLDGKTYRVQY
                     FERAVFELHPENKPPYDVLLSQLGTFRYKSETPGEQDASQGVRQVGISAGPQHYPLLA
                     GPHAAAGLNVRIYENDPQPVVDMVQDLKAKWVLHQLSWYQIEPQKDVYKWEKL"
BASE COUNT          820 a         1370 c         1381 g          871 t
ORIGIN      
        1 gacattggct tgctgactgt gacgagtgcg ccaggtgtcg gcaagaccag gcgagccttg
       61 tcagttgctc acaccgtgcc ggcagacttc ggcgacggcg tcttcttcgt ttcgcctgca
      121 gctgtctcac tgcgttcgaa atgacatagg tcggggcttc tgtactgagg tttgacaggt
      181 cttttttgag gggtcttcaa gctacaacga gcagaagagg tggtaccttc gccccgtcca
      241 tatcgtgtcg aatagccggg ggcaaggacc aggcagcgac ctcccattga cttcgtgcca
      301 gccaaaaaat tgacctgctg ctagtatcgc tagcacaact actcgtttta ctggcagggt
      361 gcgcatccag ggctaaaaat tgatgcagaa atctccgatt ttaggcaatt ttgggcattg
      421 acatttgccg caatcggtgc tactatggct gcagttatgg actctaagac agccaggcta
      481 ttgtgcctgc ctattttgcg tcctgatatt ttagtgtctc actaaatgga gaaaagcatg
      541 aaaggtaaac agagcgggca gaccgatgcc agcggttata accggcgcga tttcctcgtg
      601 aacgccagca aactggccgg ggcgatgctt gcattcccca tactcgccaa agcgccgggg
      661 gcggccgcgc agtcgaccgg ggcggggtca atattatcct cggtttcttc gagggccaca
      721 gagcgccgga cggtcggggt gctgctgcca cagtctaccg cctatccgaa gcttggcgcg
      781 aacttcctgg cgggcctcga cctctatctc aaccagtcgg gccgcaaggg agtaagcgtg
      841 gtttccgccg cggtcggcgc ttgctgcggg caagccaagc aagaggcgcg ggcgatgctc
      901 aagtcgggga aggttgacct gatggtcggc ctggtcaacc ccagcgttgc cgacaccctt
      961 gtgcccgtcc tcgaagcctc gaataccgcc ttcatcgcca gcagcctcgg cgaaaacatg
     1021 gtccgtgccg atgagcacag ctccaatatc ttccaccaca cgatgggcat gtggcaggcc
     1081 aactgggcgc tcgggaagtg ggcggccacg gccctgggcc gcacggcggt catggcctcg
     1141 tcgtattacg atagcggcta cgatacgctc tacgccttcc gcatgggctt cgaggccggt
     1201 gggggtcggg tgcactccac ccacgtgacc catcgcccca acagcgacgt gggcctcgcg
     1261 tccgtaatgt ccgccatcaa ggcggccaag ccgaacgtgg tcttcgccag ctactacggc
     1321 cagaccgcgg tcgatctgct gaatgcctac gctcgcgccg ggctatcgcg gtcggtgcct
     1381 ctcgtcggca ccgccttcct ggtagaccag gcggtcgtca attcggtcgg ttcggctgcc
     1441 gcaggtgtga cgacctctct gccctgggcc gccagcctca acaccgccga aaaccaggcc
     1501 ttcatcaccg cctaccagat caagaccggc aagtcacccg acgcgttcgc ggtgctcggc
     1561 tacgagacgg gcggtctggt cgcctcggca atcgaccgcg cagccggggg caacttgcga
     1621 gatgccctgg ccggtgccag tgtcgtaggc cctcgcggcg caatcgcgat gaatgccacg
     1681 acacagagca cgtcagcccc gcactacctc cagcaggccc agcggcaagg catcaacgtg
     1741 cgcaacgtgg tgctggccga cctggctgct gctgccgagc tcgacgggca ggtggccgct
     1801 ctaaggcaga ccacgcgcac cggctggatt agtacctacc tctgcggcta agcgccggcc
     1861 aaaaccaaat agtgagacgg cgaggggggc cacctatgtg gccctccttc ttcgcgtgca
     1921 cggccgtatg ggtacggaaa ttgctaaaga gccaggttag ctgttcgctc cgcctctcag
     1981 cgttgcgagg tacgttttca acggctgcag agtcgcggcc gcatattgaa ggaggtaagc
     2041 agtggaacct gatggcctgg attgtggtgg tacctttgtg gagttctcgt ggcatagctt
     2101 cgagaccgac ggcgagtggg acgcaattgc tcccaaagcg gaaggtgccg agttggttgg
     2161 cgtggcgggc gaccgcacgg agaccgatga cagggtagcc gaaccggcga aagagcggta
     2221 acaccggcct ctcgcgggtg tgatacctgc cggcaaaata gagaacctgg gtgcgatgcc
     2281 tgtatggggt cgcactcgct tttatgaccc cggatgtaca tctttcggtg gtgtacgcgg
     2341 caccccggtt gccctatcct ggctcttgat ggcaggtcca ggaggaatgc acaccacgcg
     2401 ggaggaggca gccatgcagg gacatcaggc agcacatgac gtggcgcgag aaccttcgac
     2461 gccttctccc aggagattgg ccttcagcgc gacggtgcat tgtttgacgg gctgcggtat
     2521 tggtgaagtg ctggggctgg tgatcggttc cgcgctcggc tggcacgatc tgccctccat
     2581 tgcgctggcg gtggtgctgg cattcatctt cggctacggg ctgacgttgc ggccgctgct
     2641 ggccggtggg ttaaggctgg cagaggccct gcgcctggcg ttcgcggccg ataccttgtc
     2701 aatcgccgtg atggaaatcg tggacaacgc catcatcctg ctgattcccg gcgctatgga
     2761 tgccgggctt accagcctgc tgttctgggg cagcctggcc gtcgcgctgg ccgttgcttt
     2821 cgtggtggcc ttcccggtca acctgtggct gatccgccag gggcgcggac acgcggtcgt
     2881 acatggcaag cacggcggtc atggcgggca tggcgggcat ggcgagacgg ttggtcatac
     2941 aggccacgac gggcagcctc acgctcattg agcaaggctg cgaccttagg gtagtagagt
     3001 agcgaaggtg tggtccctgc atgggccgcg ccttttgttt tgcagagggc cgggcggcac
     3061 cggctgtaac tgccctcgta caggtatgta cctccccctc tcttgcggct cgcctctgac
     3121 cttccttcct accccgcccg cataacagct tatttcccca tttcggtcct cccaacctgt
     3181 cctcccaacc tgtcctccca acctgtcctc ccagcgagtc ctcacgacca aaggcagttc
     3241 ggaggtttag ttctggttgg cactttacga gctttctcca gcttgggcaa gctctttctc
     3301 gtcgtctgtt ctgtcctggc agtggccgcc tccctccacg tctcagcgtc ttattctgcc
     3361 gctcccatct ttgccgagga tgtgcaggtg cgcgtagcgg actacagcta cacacccgcc
     3421 gcgctggctg tggaggcggg cacgaccgtc acctggacca acacgggcaa ggacatccac
     3481 aatatcgcca tcgaccgagg tcccgaagcc ttcgtgtcgg tggtgctgac tcccggcaag
     3541 accgcccggt ttgtttttac caccccaggc aggtaccact acttctgcga gtggcacgct
     3601 aacatgcagg gcgacgttga cgtgcgcggc cccaacggct ccaacgtcgc gcctgtcaca
     3661 cccgcggccc gcacctttgc cgaaacgggc aagatgttgc ggggcaagtt cctgcaatac
     3721 tgggacgctc acggcgggct ggcgcagcaa ggcttcccca tttccgagga gatggtggag
     3781 aaaagcgaca cggatggcaa gttctacacg gtgcagtact tagagcgggc cgtgttcgag
     3841 ctacaccctg acaaccagcc gcccaacgac gtactcctct cgctgctggg caacttccta
     3901 tacaagcaga aataccccaa cggcgcgccc ggacagcagc ccaacaccag cgcaggctcg
     3961 gtgctgttcc aggagaccgg caagcgagta ggcggccgct tcctccagta ctggcaggag
     4021 agtggcgggc tgcctcagca aggcttcccc atatcggacg agttcatgga gaagtcgcag
     4081 ctagacggca agacgtacag ggtgcagtat ttcgagcgtg cggtgttcga gctgcaccct
     4141 gagaacaagc cgccttacga cgtgctactc tctcagttgg gcaccttccg ctacaagagc
     4201 gagacgccag gagagcagga tgcgtcgcag ggcgtgcggc aggtcggtat ttcggcgggg
     4261 ccgcaacact accccctgtt ggccgggccg cacgccgccg ccggcctgaa cgtccggatc
     4321 tacgagaacg acccccagcc cgtggtagac atggtgcagg acctgaaggc caaatgggtg
     4381 ctgcaccagc tttcctggta ccagatagag ccacagaagg acgtctacaa gtgggagaag
     4441 ct
//
LOCUS       DASRWZ010000056         5230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_620852, whole genome shotgun sequence.
ACCESSION   DASRWZ010000056 DASRWZ010000000
VERSION     DASRWZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620852"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..828)
                     /locus_tag="VFH60_00790"
     CDS             complement(277..828)
                     /locus_tag="VFH60_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312344.1"
                     /translation="MRIFQPSGSEGLSNSSSADEAEEVEDDDLQISPLAMMNEGSPEA
                     WLGNFLAILGVAGSARYSTIEGSKRDETLITQLRDIAEASGRENPMRLLEGENSESLK
                     TSDWATWWRLEGELGRYAYSVLALTLLGDTTHRADLPTLYRQTANSRIQKDAHYVLCH
                     LLGKIWPGYNVTEADLRRVAAET"
     gene            complement(773..2920)
                     /locus_tag="VFH60_00795"
     CDS             complement(773..2920)
                     /locus_tag="VFH60_00795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6312345.1"
                     /translation="MTGRRRTDAEEVADLPEDQPEYDYGGLGTMIAREMAVEARRARA
                     RREFFRIEHVTPSRKGEPESKVLGMYRVTGESGREYDVLVRDPNPEHHVNRCVCLDYE
                     SNNLGTCKHIEAVLGYIRRKHGSQLRAAKPRLLEINRLTVYVTLRYVGDGTWTAAPVY
                     DHALDPGVLRLINHYLLPYLAVLEDDPDAFLKRMQRFVEEVEDFGGKVIVEPEVYDYA
                     GQVKTRNARDARRRELMDRVEAGEARLDLLKLPLYPYQAVGTLFLAFTEKALLADDLG
                     LGKTPQAIAATKLLKEWHGIKKVLVITPASVKYQWGREIERFSDESYTVVTGSKQKRE
                     QQYAADVTFTIINYELVLRDLDHITALNADLVILDEAQRIKNWRAKTSQAIKELPHPF
                     AFVLTGTPLENKLEELYSIVEFLDEKLLGPPWQFMAQHVVKDEWGGIIGYKDLAGVRR
                     TIAPILLRRRKADVLQDLPQRIDNDFWLELEPEQERLYRPLEKQLAALLRLPEWDAGT
                     SAMALRLITLLREAATAAQLVNPEVHSSRKLKELPALVEEIALEGHKMLIFTQWERMT
                     RLAEQALAGLDVKTVRLYGGLGIRARQRIIEQFMTDPQTAVFISTDAGGLGLNLQAAS
                     FVINLDLPWNPARVEQRIGRAHRIGQTQPVNVINMIAQNTIEQRVLDVLYRKQELFEE
                     ILDVELDPETGVIKTDADLPAERLRRIVELLLG"
     gene            complement(2957..3487)
                     /locus_tag="VFH60_00800"
     CDS             complement(2957..3487)
                     /locus_tag="VFH60_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312346.1"
                     /translation="MSQSNPSQPHRRAYRYNPNTPLTTRYPRTRPTGVTVLAVLNFIG
                     AGLQVLALLLLILVPPQPVFPEGLGNDPTGFGSILQRMEEEQLATQRMITALVVLVTA
                     PLSMLTGIGLWRLRPWARKLSLVLYGLAVILTLLSSYSRPLTGSSLISILVSGAAFIY
                     LLRPEVGAAFTEQSDV"
     gene            complement(3653..4156)
                     /locus_tag="VFH60_00805"
     CDS             complement(3653..4156)
                     /locus_tag="VFH60_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312347.1"
                     /translation="MSYNPPSASPLPSLWEPGDLPPLPPVPSQPERPTGVTVLAVLNF
                     ISAGLSLLLIALALLSPAPEFGPDDSFLTQLRQEAHSREATSSVISGIVGLLVCLPIA
                     IGLWRLRTWARAIAIVLSGAGVVFTLCSILSQSFTFGTLISLGIGIYVIIYLLQSHVD
                     AAFEAAH"
     gene            4289..>5230
                     /gene="cysS"
                     /locus_tag="VFH60_00810"
     CDS             4289..>5230
                     /gene="cysS"
                     /locus_tag="VFH60_00810"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET6312348.1"
                     /translation="MAEPRYNLAIYNTLTREKETFVSMRPGQVGLYTCGPTVYNFAHI
                     GNLRTYVFEDALTRVLRYDGYEVNHVMNITDVGHLTSDADEGEDKMIKAMRREGKSAW
                     DIAKFYTEAFFNDTERLNIVCPTVVCKATDHIQEMIDLIQCLFDKGHAYVIEDGVYFD
                     VSTFPGYGKLSGQTLEDLVAGARIEVNTEKRNPADFALWKKADPNHVMQWPSPWGDGF
                     PGWHIECSAMSMKYLGRTFDMHCGGVDHIPIHHENEIAQSEGCTGEQFVRYWLHAEFL
                     LINAGKKSKSSGAAGDGGRDMGEGIAPLNQSGGFLTLQ"
BASE COUNT          997 a         1595 c         1635 g         1003 t
ORIGIN      
        1 cgccacaggg ttcgccagtg gtgaggacgc cggtaccctg tgaccaagga tggtccttgt
       61 cattctgagc gcagcgaaga atctcgtcct ccagcaccca agtagcctct ttctatgcgc
      121 cagtatggtg acattcaaaa ggggaccatc ccggtggagg acgagattct tcgctgcgct
      181 cagaatgaca aaggtgaggg atggtcctgc aatagcaaca tggccgtgtt cacgaaccta
      241 tttgtagggt atcagctcac gccttccgtg tcagaactac gtttcagccg cgaccctgcg
      301 caggtccgcc tccgtcacat tgtagccggg ccagatcttg ccgaggagat ggcacagcac
      361 gtagtgggcg tccttctgga tgcgggagtt ggcagtctgg cggtagaggg tgggcaggtc
      421 tgcccggtgg gtggtgtcac ccagcagggt gagggcgagc accgagtagg cgtagcggcc
      481 aagctctccc tccagccgcc accacgtggc ccaatcgctc gtcttgaggc tctcggagtt
      541 ctcgccctcc aacaggcgca tggggttttc gcgtcccgaa gcctccgcaa tgtcgcgcag
      601 ttgggtgatg agggtctcgt ctcgcttgct gccctcgata gtggagtagc gcgccgaccc
      661 ggccacgccc aatatagcca ggaagttgcc cagccacgct tccgggctgc cctcgttcat
      721 catggcaagg gggcttatct gcaagtcgtc gtcctcgacc tcttcggcct cgtcagccga
      781 ggaggagttc gacaatcctt cggagccgct cggctggaag atccgcatca gttttgataa
      841 cgcctgtttc ggggtccagt tcgacatcaa gtatctcctc aaatagctct tgcttgcggt
      901 aaagcacgtc cagcacgcgc tgctctattg tgttctgggc gatcatgttg atcacgttca
      961 ccggctgggt ctggccgata cggtgggcgc ggccgatacg ctgctccacg cgggcggggt
     1021 tccagggcaa gtcgaggttg attacgaagg aggccgcttg caggttcaag ccgaggccgc
     1081 ccgcgtccgt ggaaatgaac accgcagtct gagggtcggt catgaactgc tctattatcc
     1141 gctgccgcgc tcgtatgccc aatccaccgt agaggcgcac cgtcttgaca tcgaggccgg
     1201 cgagggcttg ctcggccagg cgggtcatgc gctcccactg ggtgaagatg agcattttgt
     1261 gcccttccag cgcgatttct tccaccagcg cgggcagctc cttcagtttg cgcgaagaat
     1321 gcacctccgg gttgaccagt tgcgccgccg tggccgcctc tcgcaacaac gtgatgaggc
     1381 gcagggccat cgcgcttgta ccggcgtccc actccggcag gcgaagcagg gcggccagct
     1441 gcttttccag cgggcggtat agtcgctcct gctccggctc aagctccagc cagaagtcgt
     1501 tgtcgatgcg ttgcggcagg tcctgcaaca cgtcggcctt gcgccgcctc agcaagatgg
     1561 gggcgatggt tcggcgcacg cccgccaggt ccttgtagcc gataatgccg ccccactcgt
     1621 ccttgacgac gtgctgcgcc atgaactgcc agggcgggcc gagcagcttc tcgtccagga
     1681 actccactat gctgtaaagc tcctccagct tgttttcaag gggcgtgccg gtcagcacga
     1741 aggcgaaggg gtgcggtagc tccttgatgg cctggctcgt cttggcgcgc cagttcttga
     1801 tgcgctgcgc ctcgtccagg atcaccaggt cggcgttgag ggcggtgatg tggtccaggt
     1861 cgcgtagcac cagttcgtag ttgatgatgg tgaaggtaac gtctgcggcg tattgctgct
     1921 cgcgcttctg cttgctgccc gtgaccacgg tgtaactctc gtcggagaag cgttcgatct
     1981 cgcggcccca ctggtacttc acggaggcgg gggtgataac cagcaccttc ttgatgccgt
     2041 gccactcttt caacaatttc gtcgccgcaa tagcctgggg cgtcttccct aaaccgagat
     2101 cgtcggccag cagcgccttt tcagtgaaag ccaggaagag ggtgcccacc gcttggtagg
     2161 ggtagagggg cagcttcagc aggtcgagcc gcgcctcccc ggcctccacc cggtccatca
     2221 gctcgcggcg gcgggcgtcg cgggcgttgc gggtcttcac ctgccccgcg tagtcataaa
     2281 cctccggctc cactatcacc ttgccgccga agtcctccac ctcctccacg aaccgctgca
     2341 tgcgcttgag gaaggcgtcg gggtcgtcct ccagcacggc gaggtagggc aagaggtagt
     2401 ggttgatgag gcgcagcacg ccggggtcta gcgcgtggtc gtacaccggg gccgccgtcc
     2461 atgtgccatc gcccacgtag cgcaacgtga cgtagacggt cagccggttg atctccagca
     2521 ggcggggctt ggcggcccga agctgcgagc cgtgcttgcg gcggatgtag cccagcaccg
     2581 cctctatgtg cttgcacgtg cccaggttgt tcgactcgta gtcgaggcag acgcagcggt
     2641 tgacgtggtg ctccgggttg gggtcgcgca ccagcacgtc gtactcgcgc ccgctctccc
     2701 cagtcacgcg gtacatgccc agcaccttgc tttccggctc gcccttgcgc gagggggtga
     2761 cgtgctcgat gcggaagaac tcgcggcggg cgcgcgctct cctggcctcc accgccatct
     2821 cgcgcgctat catcgtgccg aggccgccgt agtcgtactc gggctggtcc tccggcaggt
     2881 cggccacttc ttctgcgtct gttcggcgtc gtccggtcat aaagtgtcgt cctggcagca
     2941 acgcgaggtc aaggggctaa acgtcggact gttcggtgaa ggctgccccg acctccggcc
     3001 gaagaaggta gataaaggcg gccccgctca ccaatatgct gataagagag gagccggtta
     3061 gaggcctgga atagctggac agcagagtca gtataacggc aaggccgtac agcaccagcg
     3121 acagtttgcg cgcccacggc ctcagccgcc atagtccaat gccggtaagc atgctcaggg
     3181 gggccgtcac gaggactacc aacgcagtta tcatgcgttg cgtggctaat tgctcttcct
     3241 ccatgcgttg cagtatggat ccaaatcctg ttggatcatt tcccagccct tcaggaaaaa
     3301 ccggctgcgg aggaaccaat atcaagagca gtagcgccaa tacctgcaac ccggcaccta
     3361 tgaagttcag cacggccagc acagtgacgc cggtgggccg cgtccggggg tagcgcgttg
     3421 taagtggagt attcgggttg taccggtatg cacgtctgtg gggctgagac gggttggact
     3481 gcgacatgga gtcctcctta ttttgcacgt tggacgtctt caaacgcttt gttgacttcc
     3541 gcacgacaca ggtggtagaa agccgcgcca aacaccatgg ccgccaggat cgcagagacg
     3601 cttacagggc gcgtgacagt gcccggccac aacacggctt ggtccatggt cctcaatggg
     3661 ccgcctcgaa ggcggcatcc acgtgggatt gcaggagata tatgataacg taaatcccaa
     3721 tgccaaggct gatgagtgtt ccaaatgtga acgactggct aagtatggag caaagcgtga
     3781 acaccacacc ggcaccagac agtacaatcg cgattgccct ggcccatgtc cgcaatctcc
     3841 acagcccgat cgctatcggc aggcagacaa gcaagcccac aatgcccgaa atgaccgagc
     3901 ttgtggcttc gcgagagtgt gcctcctgcc gaagttgagt aagaaagcta tcatcaggac
     3961 cgaactcggg agcgggagac agcaaggcga gcgcgatcaa gagcagcgac agccccgcgc
     4021 taatgaaatt gagcaccgcc agcacggtca cgccggtggg gcgctccggc tggcttggca
     4081 ccgggggcag cggtggtagg tcaccaggct cccacagcga cggtagggga gaagcggacg
     4141 gtgggttgta agacattgcc ggctccttgc agcgcccggc tgcttgaatt caggcactac
     4201 gcaactattt ccacgctggt ttataatggt agtcctatta tcccataagt tgcacgagac
     4261 agtacgagca tatgcaggag gcgtaccttt ggcggagcca cgatacaacc tagctattta
     4321 taacaccctc acccgcgaga aagagacgtt tgtgtcgatg cggcccggcc aggtggggct
     4381 gtacacctgc ggacctaccg tctacaactt cgcccacatc ggcaacctcc gcacctacgt
     4441 cttcgaggac gctctcacgc gcgtgctacg ctacgacggc tacgaggtga accacgttat
     4501 gaacatcacc gacgtgggcc acctaacttc ggacgccgac gagggcgagg acaagatgat
     4561 aaaggccatg cgccgcgaag gcaagagcgc gtgggacatc gcgaagttct acaccgaggc
     4621 tttcttcaac gacaccgagc gcctcaacat cgtctgcccc actgtcgtgt gcaaggccac
     4681 cgaccacatc caggagatga tcgacctgat acagtgcctg ttcgacaagg ggcacgccta
     4741 cgtgatcgag gatggcgtgt acttcgacgt gtccaccttc ccaggctacg gcaagctgtc
     4801 cggccagacg cttgaagacc tggtggcagg cgcacgcata gaggtgaaca cggagaagcg
     4861 caaccccgcc gacttcgccc tctggaagaa ggccgaccct aaccacgtca tgcagtggcc
     4921 ctcgccgtgg ggcgacggct tccccggctg gcatatcgag tgctcggcca tgtcgatgaa
     4981 gtacctcggc cggacgttcg acatgcactg cggcggggtg gaccacatcc cgatacacca
     5041 cgagaacgag atcgcgcagt cggagggctg cacaggcgag cagttcgtgc gctactggct
     5101 gcacgccgag ttcctgctta tcaatgcggg caagaagagc aagagcagcg gggcggcggg
     5161 cgacgggggc cgcgacatgg gcgaggggat cgctcccctg aaccagtcgg gcgggttcct
     5221 caccttgcag
//
LOCUS       DASRWZ010000057         3294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_141346, whole genome shotgun sequence.
ACCESSION   DASRWZ010000057 DASRWZ010000000
VERSION     DASRWZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141346"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VFH60_00815"
     CDS             <1..629
                     /locus_tag="VFH60_00815"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET6312349.1"
                     /translation="PATLDRLLIALRDVPSREWAWTFGALVVLWALTELFVGRLGLHA
                     VSLESDRGSALAVLASAALAWTLAMGAGVVRLLPLGADWVRWAGLGGMAAGLTLRVFS
                     ILWLGPMFTRLVQILPGHRLVTSGPYRFVRHPSYSGLLLFFTGVGLALGDWLAVAIMV
                     LVPVLGVLYRIRVEEKALLGAFGEEYQAYRCKVSSLLPVPKSAFRRDA"
     gene            822..>3294
                     /gene="mutS"
                     /locus_tag="VFH60_00820"
     CDS             822..>3294
                     /gene="mutS"
                     /locus_tag="VFH60_00820"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET6312350.1"
                     /translation="MAETPLRQQYLNIKKQYPDTILFFRLGDFYETFDNDAAIVSQVC
                     DVVLTARPTSKDQRIPMAGVPYHSAEGYIAKLVQAGYKVAVAEQLTAPTVDQATADRR
                     IRLGKKESAITANWAESGVPTHQVGKGRELVERDVVRVITPGTVTEPGMLDARRNNYI
                     AAVVSDGQAAGIAYADITTGEFATTEIRKPTGELTIAVQQEIDRLQPAELLVPGDDME
                     GPPGSMAAYRLWAKAGDDDRGKKNGHNGASGHNRNHGPALPHQTETDPHNWATDPARQ
                     RLLKHFAASSLEPFGCARLPLAIRAAGALLAYIGDTNRSALHQLTRLVTYSTEQYMNL
                     DPQTRRNLEIADSPGGRRHSLLAVLDETRTPMGSRLLARWLNQPLLDIARLRARQDAV
                     EAFFDNGTLRMEARSLLNNMPDLERLCTRAVQGTAGPRDMVAIKTALDRLPDIHATLA
                     QVTGPYAKVLAHLSNRIQPCEDLSELIGRAIADDPPAVVGNGDAIRPGFNPELDRIRA
                     MSRDAKGWIAGLEAQERERTGIKSLKVGYNKVFGYYIEISNANTVPVPPEYIRKQTLV
                     GAERYMTPELKDYESQILNAQERLSELERAAFGEVLGSVAASAPRLLQVAEALALLDV
                     FSDLAEVAVRYSYTRPHLDDGTGLHIREGRHPVVERTLRDDLFVPNDTHLDSKEAQIV
                     LLTGPNMAGKSVYIKQVAIIVLMSQTGSFVPAEEVRVGLVDRIFTRVGAQDDIATGRS
                     TFMVEMEETAYILNHATDRSLVILDEIGRGTSTYDGLAIARAVVEYIHNSPRLGAKTL
                     FATHYHELTELEKILPRVRNYNVAVS"
BASE COUNT          620 a         1153 c         1018 g          503 t
ORIGIN      
        1 tgcccgctac actggatagg ctgctcatag cactgcgcga cgtgccctcc cgcgagtggg
       61 cctggacctt cggggcgctg gtagtgctgt gggcgctcac ggaactgttc gtcgggcggc
      121 tggggctgca cgcggtctcc ctggagagcg acaggggttc cgcactggca gtgctggcaa
      181 gcgccgccct agcctggacc ctggctatgg gagccggcgt ggtgcgtctg ctgcctttgg
      241 gggccgactg ggtgcgctgg gccgggctgg ggggcatggc cgccgggctg acgctgcggg
      301 tgttctccat cctgtggctc ggcccgatgt tcacccgcct ggtgcagata ctacccggcc
      361 accgtctcgt gaccagcggc ccctaccgct tcgtgcgcca cccctcctac agcggcctac
      421 tgctattttt tacgggcgtt ggcctcgcgc ttggtgactg gctggcggta gccatcatgg
      481 tattggtgcc cgtcctgggc gtgctctacc gcataagggt agaggaaaag gcgctgctag
      541 gggcgttcgg agaagaatac caggcatata ggtgcaaggt gagcagcctg ttacccgtgc
      601 ctaaatctgc ctttcgtcga gacgcatagg aacacgttgc aacggagcct accctttatt
      661 gtcaggtgag aggacttcta ggatatacta gacgcaccag tctccaaagg agacttcgcc
      721 ccttgtaagc ccgccaattt attggcaggg cggggttggc agagcaccca ttatccgggc
      781 acccggcata aacctccccg acacatagaa gggaccccac agtggccgaa accccactcc
      841 gccagcaata cctcaacatc aagaagcagt accccgacac catcctcttc ttccgcctgg
      901 gcgacttcta cgagacgttc gacaacgacg ccgccatagt ctcgcaggtc tgcgacgtgg
      961 tgctcaccgc ccgccccacc tccaaagacc agcgcatccc gatggcgggc gtgccctacc
     1021 actcagccga gggctacatc gccaaactcg tgcaggccgg ctacaaagtg gccgtggccg
     1081 agcagctcac cgcccccacc gtggaccagg ccaccgccga ccgccgcatc cgcctcggca
     1141 agaaagaatc ggccatcacc gccaactggg ccgagtcggg cgtgcccacc caccaggtag
     1201 gcaaagggcg cgagcttgtg gagcgggacg tggtgcgcgt catcaccccc ggcaccgtga
     1261 ccgaacccgg tatgcttgac gcccggcgca acaactacat cgccgccgtc gtttcggacg
     1321 ggcaggccgc cggtatcgcc tacgccgaca tcaccacggg cgagttcgcc accaccgaaa
     1381 tccgcaagcc caccggggag ctaaccatag ccgtgcagca ggagatagac cgcctccaac
     1441 ccgccgagct gctcgtgcct ggcgacgata tggaggggcc gcccggcagc atggcggcct
     1501 accggctgtg ggccaaagcg ggcgatgacg accgggggaa gaagaacggg cacaacggcg
     1561 cgagcggcca taatcgcaac cacggccctg ccctgcccca ccagaccgag accgaccccc
     1621 ataactgggc gaccgacccc gcccgccagc gcctgctcaa gcatttcgcc gcctcgtccc
     1681 tcgagccgtt cgggtgcgcc aggctgccgc tcgcgataag ggccgccggg gctttgctgg
     1741 cttacatagg cgacaccaac cgctcggccc tgcaccagct tacccgcctg gtcacatact
     1801 ccaccgagca gtacatgaac ctcgacccgc agacccgccg caacctggag atagccgaca
     1861 gccccggcgg gcgcaggcac tccctcctgg cggtgctcga cgagacccgc accccgatgg
     1921 gttcacgcct gctcgcccgc tggctcaacc agcccctgct cgacatcgcc aggttgaggg
     1981 cgcggcagga cgcggtagag gcgttcttcg acaacggcac cctgcgcatg gaagcccgct
     2041 cgctgctcaa caacatgccc gacctggaac gcctgtgcac ccgcgccgtg cagggcaccg
     2101 ctggcccccg cgacatggta gccatcaaga ccgccctcga ccgcctgccc gacatccacg
     2161 cgacgctggc gcaggtcacc ggcccctatg caaaggtgct ggcccacctc tccaaccgca
     2221 tacagccctg cgaggacctg tcggagctga taggccgcgc catagccgac gacccgcccg
     2281 ccgtggtggg caacggcgac gcgatacgcc ccggcttcaa cccggaactc gacaggatac
     2341 gggctatgtc gcgggacgcc aagggctgga tagccgggct ggaagcgcag gagcgagagc
     2401 gcaccggcat caagagcctc aaggtgggct acaacaaggt gttcggctac tacatcgaga
     2461 tctcgaacgc caacacggtc ccggtgccgc cggagtacat acgcaagcag acgctcgtgg
     2521 gcgcggaacg ctatatgacg ccggagttga aggattacga gagccagata ctcaacgccc
     2581 aggagcgtct gagcgagctt gagagggccg cgttcggtga ggtgctaggc agcgtagccg
     2641 cctcggctcc caggctgtta caggtggccg aggcgcttgc cctgctcgac gtgttcagcg
     2701 acctggccga ggtggccgtg cgctactcct acacccgccc ccacctggac gacggcaccg
     2761 gcctgcacat acgcgagggt cgccacccgg tggtcgaacg caccctgcgc gacgacctct
     2821 tcgtgcccaa cgatacgcac ctggacagca aggaagcaca gatagtgctg ctcaccgggc
     2881 cgaacatggc gggcaagagc gtgtacatca agcaggtggc gataatcgtg ctgatgtcgc
     2941 agaccggctc gttcgtgccc gccgaggagg tgcgcgtcgg cttggtggac cgcatattca
     3001 cccgcgtggg cgcgcaggac gacatagcca ccggccgctc gaccttcatg gtggagatgg
     3061 aggagaccgc ctacatcctc aaccatgcca ccgaccgctc gctcgtcatc ctggacgaga
     3121 taggtcgcgg cacctccacc tacgacggcc tggccatcgc ccgcgcggtg gtggagtaca
     3181 tacacaactc cccccgcctc ggtgccaaga cgctgttcgc cacccactac cacgagttga
     3241 ccgagcttga gaagatactg cccagggtgc gcaactacaa cgtggcggtg agcg
//
LOCUS       DASRWZ010000058         1515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_209772, whole genome shotgun sequence.
ACCESSION   DASRWZ010000058 DASRWZ010000000
VERSION     DASRWZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209772"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..>1515
                     /locus_tag="VFH60_00825"
     CDS             122..>1515
                     /locus_tag="VFH60_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312351.1"
                     /translation="MRYLRDKRGIVVALTLLLALGITAGAMLVRWQGSSPNAATAFEP
                     YVPEPGTGAGESKELHSMEDYWHTRVSYPTGRFDGRWLLEASEQDKLVQERVPAGRVI
                     YNRGNGASPLALDPNSWTSLGPKPLESNGCLNCFQYGHVSGRVNDIIIDPTNSTIAYL
                     ATIAGGVWKTTNCCTANTTWTPMTDDPLISTTSIDDLSMDPTNHNTIYAGTGDLNFGS
                     FSMGSAGILKSTDAGVTWAIKGADVFTGYYPEPAGTFPQYQAVGKIETDPNNGNTLVA
                     GTKTGVYFSYNGGDTWSGPCLTNSFSSQRQDITGLVLTDAGSTTDMFVAVGSRGYSTT
                     VQYNLAENGANGIYKSTVPASGCPASWSLLTRPDNGWPAGTGSGIPVYQAGGNTLGRI
                     DIDISNSNPNYIYAIVQAIGTYQGGVKGVWRSIDGGANWEMRAGLGNSATEWTGCART
                     GQQNWYNQHIEIDPL"
BASE COUNT          313 a          529 c          440 g          233 t
ORIGIN      
        1 tcctccctct tcggcgggcc tctcgaaggg tcgcactcct ctccacatgg cagaccctgg
       61 cgggattggt gcgccacgac aaccggtctc tttattttgc aaggaaaggg aaaggaagag
      121 tatgcggtat ctcagagata agcgcggcat cgtagtcgcg ctcacattgt tgctggcgct
      181 cggcataacc gccggggcca tgctcgtgcg ctggcagggg tctagcccca acgccgccac
      241 cgccttcgag ccatacgtac cggagcctgg cacgggcgcg ggcgagtcca aagagcttca
      301 cagcatggag gactactggc acacccgcgt cagctacccc accggcaggt tcgatgggcg
      361 ctggctgctc gaagcctcag agcaggacaa gctggtgcag gagcgcgtgc ccgcaggccg
      421 cgtcatatac aaccggggga acggcgcatc gccccttgcc ctcgacccca atagctggac
      481 ctccctcggc cccaagccat tggagagcaa cggctgtcta aactgcttcc agtacggcca
      541 cgtttcgggg cgcgtcaacg acatcatcat tgacccgacg aacagcacca tagcctacct
      601 ggcgacgata gcgggcggcg tgtggaagac caccaactgc tgcacggcca acaccacctg
      661 gacccccatg accgacgacc cgctgatctc cactacctcc attgacgacc tctcgatgga
      721 ccccaccaac cacaacacca tctacgcggg cacgggcgac ctcaacttcg gctccttctc
      781 gatgggcagc gcgggcatcc tcaagagcac cgacgcgggc gtgacctggg ccatcaaggg
      841 cgcggacgtg ttcaccggtt actaccctga gcctgccggg accttcccgc agtaccaggc
      901 cgtaggcaag atagagaccg accccaacaa cggcaacacc ctcgtggcgg gcaccaagac
      961 gggcgtctac ttctcgtaca acggaggcga cacctggtcc ggcccctgtc tcaccaactc
     1021 attctcctca cagcgccagg acataaccgg cctggtgctg actgacgcgg gcagcaccac
     1081 cgatatgttc gtggccgtag gctctcgcgg ctacagcacg acggtgcagt acaacctggc
     1141 cgagaatggc gcgaacggca tctacaagtc caccgtgccc gcaagcggct gcccggcctc
     1201 ctggagcctt cttacccggc ccgacaacgg ctggcccgca ggcaccggca gtggcatccc
     1261 cgtctaccag gcgggcggca acacgctggg ccgcattgac atagacatct cgaacagcaa
     1321 ccccaactac atctacgcta tcgtgcaggc tatcggcacg taccagggcg gcgtcaaggg
     1381 cgtgtggcgc tcgatagacg gcggcgctaa ctgggagatg cgcgccggcc tcggcaacag
     1441 cgccaccgaa tggacgggtt gcgcccgcac cggccagcag aactggtaca accagcatat
     1501 agagattgac ccgct
//
LOCUS       DASRWZ010000059         2918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1169587, whole genome shotgun sequence.
ACCESSION   DASRWZ010000059 DASRWZ010000000
VERSION     DASRWZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169587"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2779)
                     /locus_tag="VFH60_00830"
     CDS             complement(<1..2779)
                     /locus_tag="VFH60_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312352.1"
                     /translation="MLLLLAWSATWLGTSAQAWPPLDGVVSDDTGRLDAGEINQAAAR
                     LEDMGVKPLVVFSQGGRGFGSDAAGLGQAAAQQYGLSSAPGVLDPDLLAIVVILDSRQ
                     ASIIYGDRLKSALEQRRGSGTLADQIRSDYLNPNLAGGDFTGAYVDSINYAADQIDLF
                     RNPPPTATPQPAVVTQVSTEGIGDALLWIFLGIVVVIGLAILGPVLWRNYKRNQERAT
                     RIRTLREQLAQARNVAADMITSLDFPADPNEQIQYRFLALALGRERPQQLAQLTGQYK
                     TIYASMSDALARFDLLNERTFNTEEELTQGIAEYQWVQATYKNAGDFLQHLSTAGKEV
                     EGQMAAAPGEIDAAKKAIAAATDELARLAAAAPDLTLPGAEAFSAPLWSPLREAESAL
                     RAAPPLPLRAHDRARTARTLAEATHQSVVELAQAHARLAQERERLAATRREGFKLASA
                     DADFEAALAALNGAAGLLGPEQREQFAKALTKATQAVQVAGENVSSQLALHAANEKAL
                     AELEAAGEQLRAYIQEGVKAFDVVDEYAPSSWEDIRGNGTEAQKRGDDAFNLWQRATR
                     LNGATPDGEQDFTEAAADIAEANTLIAEARALVAAIIERLDHLRKSQAIAQQEIAAAE
                     KDIAAGRAYVSQYDPDITPRPDDMLAQAEKQLAAAREEVSQARPDWIRVVELARAAND
                     LSDRALADARSQAEAMEARRRRVQTTYEQALASLSRLRNFVQVHAGDIHPSINKAVGD
                     ADATFKRAQEELEPLRAGGLEDVARAEALDRVAEGYGKAQKIADEAYKLAYDQFQVMD
                     GLRKQASSAIEQARRSIQDARNYLQDSQEVLSGRPVGYLHQAISLMPKWRDKADAQEL
                     RSIQAAANKANERANLAIQTASAEIDEYNEQIREREAADNAAQMALLVGVLDAVVSSA
                     GSRRSSGGGWGS"
BASE COUNT          381 a         1010 c          943 g          584 t
ORIGIN      
        1 ggctacccca gcccccgccg ctgctgcgac gactaccggc gctgctgact actgcatcta
       61 gcacgcccac gagcaacgcc atctgggccg cgttatccgc ggcttcccgc tcgcgtatct
      121 gctcgttgta ttcatctatc tcggcgctgg ctgtctgtat ggcgaggttg gcgcgttcgt
      181 tggccttgtt cgccgctgcc tgaatcgacc ttagctcctg cgcgtcggcc ttgtcgcgcc
      241 acttcggcat gaggcttatc gcctggtgca ggtagccgac cggcctgccg ctgagcacct
      301 cctggctatc ttgcaggtag ttgcgtgcgt cctggatcga tctcctggcc tgctcaatcg
      361 cgcttgacgc ttgcttgcgc aggccgtcca tgacctggaa ctggtcgtaa gctagcttgt
      421 acgcctcgtc cgctatcttc tgcgccttgc cgtagccttc cgcgacccgg tcgagcgcct
      481 cggcccgcgc cacgtcttcg aggccgcctg ccctgagcgg ctccagttct tcctgggcgc
      541 gcttgaacgt ggcgtcggcg tccccgaccg ctttgttgat ggaagggtgg atgtcgcccg
      601 cgtgtacctg cacgaagttg cgcaggcggc tcaggctggc tagcgcctgc tcgtacgtgg
      661 tctgcaccct gcgcctgcgg gcttccatcg cctctgcctg gctgcgagca tcggccagcg
      721 cgcggtcgga caggtcgttg gccgcgcggg caagctccac cacccttatc cagtcggggc
      781 gggcctgcga cacctcttcc ctggcagccg ctagctgctt ctcggcctgc gccagcatgt
      841 cgtcgggccg gggcgtgatg tcggggtcgt actggctgac gtaagcacgc cctgccgcta
      901 tgtccttctc ggcggccgcg atttcctgct gcgcgatagc ctggctcttg cgcaggtggt
      961 ccaggcgctc gataatggcc gccaccagcg cccgcgcctc cgcgatgagg gtgttggcct
     1021 ccgcgatgtc ggccgccgct tccgtgaagt cctgctcacc gtcgggcgtg gccccgttca
     1081 gccgggtagc gcgctgccac aggttgaacg catcgtctcc ccgcttctgg gcctcggtgc
     1141 cgttgccgcg tatatcctcc cacgaactgg gggcgtactc gtcaaccacg tcaaacgctt
     1201 tcacgccctc ctgtatgtac gccctcaact gctcgcccgc ggcctccagc tccgccagcg
     1261 ccttctcgtt ggccgcgtgc agggcaagct ggctgctgac gttctccccc gccacctgga
     1321 ccgcctgggt agccttagtc agggccttgg cgaactgctc gcgttgttcg gggccaagca
     1381 ggcccgccgc cccgttcaac gcggccaggg cagcctcgaa gtccgcgtcg gcgcttgcca
     1441 gcttgaagcc ctcacggcga gtggccgcca gcctttcgcg ctcctgggcc agccgggcgt
     1501 gtgcctgggc aagctccact acagactgat gagttgcttc cgcgagagtg cgtgcggtgc
     1561 gtgcccggtc gtgggcgcgt aggggcagcg ggggtgcggc cctgagcgcg ctttctgctt
     1621 ctcgcagggg ggaccacagg ggtgcggaga acgcttccgc gcccggtagt gtgaggtcag
     1681 gggccgccgc agccaaccgt gccagttcgt cagtcgctgc ggctatcgct ttttttgccg
     1741 cgtctatctc ccctggcgcg gccgccattt gcccctccac ctccttgccc gccgtggaca
     1801 ggtgctgcag gaagtcgccc gcgttcttat acgtggcctg cacccactgg tactcggcta
     1861 tgccctgggt aagctcctcc tccgtgttga aggtgcgctc gttcagcagg tcgaaccggg
     1921 cgagggcgtc gctcatgctc gcataaatcg tcttgtactg gccggtaagc tgggcgagtt
     1981 gctggggccg ctccctgccg agcgccagtg cgaggaagcg gtactgtatc tgttcgttgg
     2041 ggtcggcggg gaagtcgagg ctggtgatca tatcggcggc cacgttgcgc gcctgggcca
     2101 actgctcccg caacgtccga atcctcgtcg cccgctcctg gttccgcttg tagttccgcc
     2161 agagcaccgg ccccagtatc gcaaggccga ttaccaccac aatgccgagg aatatccaga
     2221 ggagcgcgtc cccaatcccc tccgtactga cctgagtgac cacggcgggc tggggcgtgg
     2281 cggtgggggg cgggtttcgg aaaaggtcga tctggtccgc cgcgtagttg atgctgtcaa
     2341 cgtacgcccc cgtaaagtcg cctccggcca ggttggggtt gaggtagtcc gacctgatct
     2401 gatcggccag ggttccgctc cccctgcgct gttccagggc cgacttcagg cggtcgccat
     2461 aaatgatgga cgcctgccgg gaatcgagaa ttaccactat agccagcagg tcggggtcca
     2521 gcacgccggg ggccgaagac agcccgtact gttgtgctgc ggcctgccca agccccgccg
     2581 cgtccgagcc gaaaccccgc cccccctgcg aaaagaccac gagcggcttg acgcccatgt
     2641 cttcaagccg agcggcggcc tggttgatct cgcccgcgtc gagcctgccc gtgtcgtcgg
     2701 ataccacgcc gtcgaggggc ggccatgcct gcgcggatgt gcccagccag gtggcggacc
     2761 aggcgaggag caatagcaac gcggctagga cccgaacagc gccgggcctc cacgctccca
     2821 tcgtgattgt aggtcgggtg gggggcaacg ctatactacc cctgcgtctc ggttgcctgg
     2881 gtgcccgcct ggctcaaatc gcgctggccc tcgttgag
//
LOCUS       DASRWZ010000060        10118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1032432, whole genome shotgun sequence.
ACCESSION   DASRWZ010000060 DASRWZ010000000
VERSION     DASRWZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032432"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFH60_00835"
     CDS             <1..430
                     /locus_tag="VFH60_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312353.1"
                     /translation="VTPGYVFVFNATTNTVTLTLNSHPVANGTLYGTNISSKYKPNVV
                     PIRRVSGSGNPQVAQFGGQNQLVVGFPTGGSVQYEVDTNTYLQNQIGFSLQLYIFYSV
                     VVLVWPGQQESMPGQSQVLFPQSVSLGPGESQLLSSNGDS"
     gene            690..1949
                     /locus_tag="VFH60_00840"
     CDS             690..1949
                     /locus_tag="VFH60_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312354.1"
                     /translation="MTDVSISGNGNYQIYPAFPGTDSGCENATFLLPPSQFTYPNTIT
                     WTISLIYNGPASVPSGYSNDDFALAFQQPGSTPQSVLVQITLKGTDQNPKANMWACAP
                     TARTALMANFVDFVTQIEKQFELPGLLVPGSTFRIAQQIADNMPAPLLETLFYRYSFS
                     PGFAPGTLPYVDVRPGMRLRVETQISQFLTPDSPMNGYVGGGTFYYQVNSVPAGNGTR
                     VVAFDPFLGTIKAPSVQGAASSPLVAGGLIDLQLAAGAFPHWRLFYPSSVQPPLQSGD
                     TDVTDNVTLVGAQSLEALGTITNDQPSSTTCAATGVGCTVFLGRTIAVPEIPVWIVDN
                     LYHLPALQYVPVGTTLANLVERFIPLPVDPNIAWSAVTTNRISTAAGSLGSVAINPAS
                     QTSPSPVPSTMWDVPLMGGDSVNFNNF"
     gene            1984..5421
                     /locus_tag="VFH60_00845"
     CDS             1984..5421
                     /locus_tag="VFH60_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312355.1"
                     /translation="MQFSQTNAVPQLYQASDNGAATPGTLLYLLPKGTSPPGPTIPFA
                     AAWTTYQGTYIFLEQPLTSGTEQTFANNAWTFINDPRRQGTRFVWFNQPAGSGPLSGT
                     AIAVYQPTGSQSYATGFPVTFPFQNVSLFIPAKSIIGRDDTNFAFNFTPPAGQKITLS
                     AKSGKVMITAGPPVTLPFNGNLAGCLQFSLQLTQQNLTDLDVGLRYYYAQTSGDTVAA
                     EPGEAGDAVEAGADNSFFLSSLRYPLFNQGLTLYANIDPLAPINASGQMRTFFAFNGA
                     DAGQPSAGQAAPVSAYMNSTMGYAFKLLPLTGNQVSTGFAALVFAPNQQASAPSAHDP
                     LYLVPSGDFALQPTGTGQANLMGGLSGVEYFALGGSTNLVSFFPGKSAFAEGFVPGKP
                     PGSTKLKPTIAPTTSFALVSSQAAAVDYYAQPDQSVLYNYSQNVTPAEKAISSLALVP
                     VQAATIPSPEPKLAFPLLPYSGIGNQDLSVYQQMESQMVGPARRQALLLAPPVAKQQL
                     TAAASGQSKYSTTPQGLLATYVPGGPPTNWSEIILAQMGALGDPQFVGLTNVTGDLLT
                     AFQTNKMFLVISQPSSVTGNLLKDNAQIVIGSSEGNNWNFNLDPAVWSQPPGGTILII
                     KFYDMSIKDLAGQTSLWSCPTTFNQDAAATGSAIKGIIDAVDKNDPDFATFLNAVTDP
                     KWNGILALNVEAPLNQLPAQLQGLAAGIDASQFFAHHIGINASKINVPSGGGDLGISN
                     SSIFGMINYTAPSGSAIEEGGGDYQFEVEQLKVLFLNSAVAGFSSIIDLQVNVLFGEP
                     STLIGSPNNIVQLYGVYQQQGSQGEGSYIFQTKSGQPSDFNMTSNVLNAVRLSKGQDV
                     TVTSDSKSTETEAQFLFWGLLDFKGLEGFDLFSFGRESGATAPAGLSFGNLIVEMTFD
                     NTQAQPVPSFTFDAGQLSFDIAGSQARKGGFFNHFPLTVAGFTQAKEGTTPASLGYMG
                     VQSPLTQSSLAYPWYSLNFNLNLGAPGALAAQAGFVASVAAAWTPNLGQDYTVFTGLK
                     LPGSSGSKRQITIEGIFNITFKTLEIIVLPDSNTYILVLYGIGFKFLSFTFPPTGQVN
                     FVLFGDPNSSASGNTSVGWYAAYAKPDAKKSAGSTNSGQLPGPSLMLPIDVPGGRGR"
     gene            5418..6617
                     /locus_tag="VFH60_00850"
     CDS             5418..6617
                     /locus_tag="VFH60_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312356.1"
                     /translation="MIRIMAWNIERFGVNKLQANAWDTTGSRTAFITSTIAQANPDVL
                     VIIEVQTNAVAGFGGLISDTSGAPAVQALLWWLRGWQPGAGWSVVPPLIISPGGGYSE
                     GIAVFFKSANLSFWGPWMWNGVTGAPLVLGMQQTYAGTTWDGYLPAAASTRPGPVNQD
                     TLAGQYRFFDAMGNVINFPNATSRSVWMTQFQVLATGRGLSLFSVHFPPKVAQSRQAF
                     GQLARVPEITSALVAEDRVVLGDFNINALNGWEAGVFQHLTGGPGVIGGVPVSPINYT
                     QQFAAATSIKSVKQASTTGAAPYYSYAKLSSAGALMAIDNMFVARAGGAAGAAGNAQV
                     ANRVIGTAAAPVAYTMDMVSSIPAIIGGLPPGGARKRRLNTLVNYGHIGGGRGSSDHM
                     PVVIDLP"
     gene            6638..>10118
                     /locus_tag="VFH60_00855"
     CDS             6638..>10118
                     /locus_tag="VFH60_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312357.1"
                     /translation="MATNTLSELAAYLQAQAQQTTGVTLDSTFLDTRVQGLIADDLLL
                     AGKAITLAQVTPKNVFVSGNTLYIVNAQTPASGALLHLKSVRANVAIQQTTLNNPTGY
                     DLVLQLVLPGEWNFGVSFPDLALLESPGYLDLDTGPYFYFSSFEAAQPLPSFPAPPKS
                     QELALAEPTEAAGNNILLTGLNYYSTVKIGGIFGVLATIIHENDPLPMYGLVTSTSGE
                     TNFTLKAYLSQTSVGIGFISASSPFLGVGVYYPPAEAGDDSAGGEQEARALALLDEDE
                     EDAEGDDESYPMLLYYLGCTITITSQDGEKLPFEIDVSIPDLQSQQVAVSIVSDQSKP
                     LNLLNLASVLMNSTDTAISTAVKPLDQYLGTITLEQFTAIFQPSATTPLRSIQVQIGT
                     DPPWDTHIPSPSDSSNTLKLDLSLSWAIMFGPATPTWSVSFDALLIFDPKLSFEVTVT
                     VPNLMITGAEHGTVTLSLSDLNTIFHTNLPIPDDLLTMSFTDFMIGMDVNNKKYTVTG
                     TINGQFALFGTPILGLKDMVVGLALDTKASTYTLNLDGIVALGSIEVQTSATISNAAN
                     TDTVFSMHLVNETVGSMLNHLVHLVDPDYDISFGEPWNKLLDISLDALVLAVNVTKGT
                     VSLTYNATIDLGFMEITGVTLTYTKGTTAASSVQISLDGTFLGQSFGGNSGKPGLGWD
                     AINDNPPAVPGQGSSLFDLRYAGLGQHIAFTGIDLNTVEDVMKALKASVLPSQSGKLP
                     PFGQNSLAFSQQSNWLIGAEFTVMGTVSISAIFNDPNLYGILIALSGEKAKSFAGLSF
                     EILYHKVTDTIGVYHIELKLPDAMRHLEFGEVSITLPIVILDIYTNGNFRVNFGFPKG
                     LDFSNSFAIQVFPFVGYGGFYFALLNGATSTRVPKIINGNFSPVIEFGIALSLGVGKT
                     VDEGILSGGITVTVIGILQGVLGWFNPTDPSPKETYYWIQGTIAIVGKLYATINFGII
                     SASLDVTAYASVTLTIESHQPIYIAISAGVSVRVSVKIIFFTIHLSFSAHINASFTIG
                     SASPTPWKLASGSSVSSNGQLRQLRGQQTLHSTLPLHAGYYRALRRSLIGTPAPLSNW
                     PAVLVLQSLSAIDPAANSGASRTGGTTTISTTAAHDYVVGDQIKVAGVADGSFNGTFG
                     VASVTSTTFTYAQA"
BASE COUNT         1920 a         3417 c         2943 g         1838 t
ORIGIN      
        1 cgttactccc gggtacgtct tcgtcttcaa tgcgaccacg aataccgtga cgttgacgct
       61 caactcgcat ccggtagcga atggcaccct ctacgggacc aacatctctt caaagtacaa
      121 gcccaacgtg gtccctatca ggcgcgtctc cgggagcggg aacccccagg tcgcccagtt
      181 cggcgggcag aaccagctcg tggtgggctt ccctacgggc ggctccgtgc agtacgaagt
      241 tgacaccaac acgtacctac agaaccagat aggctttagc ctgcagctat atatcttcta
      301 ttccgtcgtc gtccttgtgt ggccggggca gcaggaaagc atgccgggcc agtcccaggt
      361 gctgtttccg cagtccgtaa gcctggggcc tggcgagtcg cagctcctat ccagtaacgg
      421 cgattcttga ttccgcaaca ggtgcaggtc caatatgtcc tgcgcctgcc attgtatgtc
      481 tattctgccg tgccgaggct cctaacactc gcaaccgggc caggacagcc tcttaccccg
      541 gcgataattt acgcaggcac caatagcagc tagccagcgc agagcagcgg gccgggcacc
      601 tgaaggctcg ctcgccaacg ccagaccagc taagaaggag gcgcgcaagc cgggcggtta
      661 cacgccgctc tacttggcgc acgatgagta tgacagatgt ttccataagc ggaaatggca
      721 actaccagat ctaccctgcc tttccgggca ccgactccgg ctgcgagaac gcgacttttc
      781 tgctgccgcc atcccaattc acctatccaa atacgattac ctggactatc tcgctgatct
      841 acaacggccc ggcctcggtg ccgtcggggt atagcaacga cgatttcgcg ctggcgtttc
      901 agcagcctgg cagcacgccg cagtcggtcc tcgtgcagat caccctcaag ggcaccgacc
      961 agaacccgaa ggccaacatg tgggcgtgcg cccctaccgc gcggaccgcc ttgatggcga
     1021 acttcgtgga ttttgtgacc caaatcgaga agcagttcga gttgccgggg ctgctcgttc
     1081 cggggtcaac cttccgcatc gcgcagcaga ttgccgacaa tatgcccgcg ccgctgctgg
     1141 agaccctctt ttaccgttac tcgttctcgc ccggcttcgc acccggtacc cttccctacg
     1201 tggacgtgcg ccccggtatg cgcctgcgcg tggagaccca gataagccag ttcctcaccc
     1261 ccgactcgcc catgaacggg tacgtggggg gcggtacgtt ctactaccag gtcaattcgg
     1321 tccccgccgg taacggcact cgcgtggtgg ccttcgaccc gttcctcggc acgatcaaag
     1381 cgccgtcggt gcagggtgcc gcaagctctc cactggtcgc ggggggcttg atcgacctgc
     1441 aactggccgc cggggccttt ccccactggc ggctgtttta cccctcgtcg gtgcagccgc
     1501 ctctgcaatc cggcgatact gacgtaaccg acaacgtgac cctggttggc gctcagagcc
     1561 tggaagcgct cggtacgatc acgaacgacc agccatcttc gaccacctgc gccgccaccg
     1621 gtgttgggtg caccgtcttt cttggccgca ctatcgccgt gccggagata cccgtctgga
     1681 tcgttgacaa tctttaccat ctacccgcgc tccaatacgt gccggtcggc actaccctgg
     1741 cgaacctggt ggagcgattt attccgctgc cggtagaccc taatattgcg tggtcggccg
     1801 tcaccaccaa tcgcatttcg accgcggcgg ggtcgctggg gagcgtggcc atcaacccgg
     1861 cttctcagac aagcccttcg cccgtcccct cgaccatgtg ggacgtgccg ctgatgggcg
     1921 gggacagcgt caactttaac aatttctgat ccggccggga cgcagaggac aggagcacgc
     1981 cagatgcagt tcagccagac aaacgctgtg ccccagcttt accaggcaag cgacaatggg
     2041 gccgccactc ccggcacgct cctttacctg ctgccgaagg gcacctcccc gcccggcccc
     2101 accatcccgt tcgcggccgc gtggacgacc taccagggga cctacatctt cctggagcag
     2161 ccgctcacgt ccggcacgga gcagaccttc gccaacaacg cctggacgtt catcaacgac
     2221 ccgcgccgcc agggcacgcg cttcgtgtgg ttcaaccagc ccgccgggtc gggtccactg
     2281 agcggcaccg ctattgccgt gtaccagcct acgggtagcc agagctacgc cacggggttc
     2341 cccgtgacct tccctttcca gaacgtaagc ctctttatcc ctgccaagtc gattatcggc
     2401 cgcgacgata ccaacttcgc cttcaacttc acgccgcctg cgggccagaa gatcacgttg
     2461 agcgcgaagt cgggcaaggt gatgattaca gccgggccgc cggtcacgct gcccttcaac
     2521 gggaacctgg ccggttgcct gcaattcagt ctgcaattga cccagcagaa cctgaccgac
     2581 ctcgacgtgg ggctgcgcta ttactacgcc cagacctccg gcgatactgt ggcggcggaa
     2641 cctggcgaag caggagatgc tgtcgaagcc ggggctgaca actcgttctt cctctcgtcg
     2701 ttgcgctacc cgcttttcaa ccaggggctg acgctctacg ccaacatcga cccgctggca
     2761 cctatcaacg cgagcgggca gatgcgcacc ttcttcgcgt tcaacggtgc cgacgcgggg
     2821 cagccgtcgg cggggcaggc cgcgcccgtg agcgcgtaca tgaactccac tatgggctac
     2881 gcgttcaagt tgctcccgct gaccggcaac caggtatcga ccggcttcgc ggccctcgtg
     2941 ttcgcgccca accagcaggc gagcgccccc tccgcgcacg acccgttgta cctggtgcct
     3001 agcggcgatt tcgccctcca gccgacgggg accggccagg ccaacttgat gggcggcctc
     3061 tcaggcgttg agtatttcgc gctggggggc agcaccaacc tggtgtcctt cttccctggc
     3121 aaatccgcgt tcgccgaggg ctttgtgcct gggaagcccc ctggctcgac caagttgaag
     3181 cctactatcg caccgaccac gtccttcgcg ctggtaagct cgcaggccgc cgcggtagac
     3241 tattacgccc agcccgacca gtcggtcctc tacaactata gccagaacgt gaccccggcc
     3301 gagaaggcga tctcctcgct cgccctggtg cccgtgcaag cggcgacgat cccctcgcct
     3361 gagcctaagc tcgcgttccc gctgctgcca tactccggca tcggcaacca ggacctgagc
     3421 gtgtaccagc agatggagtc gcagatggtg gggcctgccc gccgccaggc gctactctta
     3481 gcccctccgg tggcgaagca acagcttacc gctgccgcga gcggccagag caagtacagc
     3541 acgaccccac agggactgct cgccacgtac gtgcccggcg gcccgcccac caattggtcg
     3601 gagatcatcc tggcgcaaat gggcgcgctg ggcgacccgc agttcgttgg cctcaccaac
     3661 gtgaccgggg acctgcttac ggccttccag accaacaaaa tgttcctcgt catctcacag
     3721 ccctcctctg ttaccggcaa cctgttgaag gataatgcgc agattgtcat cggcagcagc
     3781 gagggcaaca actggaactt caacctagac cccgccgttt ggtcgcaacc gccgggtggg
     3841 acgatcctca tcatcaagtt ctacgatatg agcatcaagg acctcgcggg gcagacgagc
     3901 ctctggtcct gcccgaccac cttcaaccag gatgccgctg ccaccgggag cgccatcaag
     3961 ggcatcatcg acgcggtgga caagaacgac cccgatttcg ctacgttcct caacgcggtg
     4021 accgacccga agtggaacgg tatcctggcg ctgaacgtgg aggccccgct gaaccaactg
     4081 cccgcccagt tacaggggct ggcggcgggc atcgacgcca gccagttctt cgcgcatcac
     4141 atcggcatca acgcctccaa gatcaacgtg ccctcgggtg ggggcgacct gggcatctcc
     4201 aactcctcga tcttcggcat gatcaactat acggccccgt ccggctcggc catcgaggag
     4261 ggcggcgggg actaccagtt cgaggtggag cagctcaagg tgctcttcct gaactcggcc
     4321 gtggcgggct tctccagcat catcgacctg caagtgaacg tcctcttcgg tgagccgagt
     4381 acgctaatcg gatcgcccaa caatatcgtg cagctttacg gggtgtacca gcagcagggg
     4441 tcgcagggcg aaggctcgta catcttccag acgaagtccg gccagccctc cgacttcaac
     4501 atgaccagca acgtgctcaa cgcggtgcgg ctctccaagg ggcaggacgt cacggtcacc
     4561 tccgatagca agtccaccga gacggaggcg cagttcctct tctgggggct gctcgacttc
     4621 aaagggcttg aggggttcga cctcttctcg ttcgggcgcg agtccggcgc gacggcaccg
     4681 gccggtctga gcttcggcaa cctcatagtc gagatgacct tcgacaacac gcaggcccag
     4741 cccgtgccgt cgttcacctt cgacgccggg cagttgtcgt tcgacatcgc gggcagccag
     4801 gcgcgcaagg gcggcttttt caaccacttc ccgctcactg tggccgggtt tacgcaggcg
     4861 aaggagggca cgacccccgc cagcctcggc tacatgggcg tgcagtcgcc gctgacgcaa
     4921 agctcgctcg cctacccctg gtactcgctg aatttcaacc ttaacctggg cgcgccggga
     4981 gcattggcgg cccaggccgg gttcgttgcc agcgtcgcgg cggcgtggac ccccaacctg
     5041 gggcaggact acacggtctt tacggggctg aagctgccag gctccagcgg ctcgaaacgc
     5101 cagatcacga tagagggtat cttcaatatc accttcaaga cgcttgagat catcgtgctg
     5161 ccggacagca acacctacat cctggtgctc tacgggatcg gcttcaagtt cctcagcttc
     5221 accttcccgc cgacgggcca ggtcaatttc gtgctctttg gcgaccccaa ttcgagcgcg
     5281 agcggcaaca ccagcgtggg ttggtacgcc gcttacgcca agccggacgc gaagaagagc
     5341 gccggcagca ccaactcggg ccagcttccg gggccgagcc tcatgctgcc tatcgacgta
     5401 ccgggaggca ggggccgatg atacgcatca tggcatggaa catcgagcgg ttcggggtca
     5461 acaagctgca agccaacgcc tgggacacca ccggcagccg caccgcgttc atcacttcga
     5521 ccatagccca ggccaacccg gacgtactgg tcattatcga ggtgcagacc aacgccgtgg
     5581 cgggatttgg cggcctgatt agcgacacgt cgggagcccc ggccgtccag gctctactct
     5641 ggtggctgcg cgggtggcaa cccggcgccg gttggagcgt ggtgcctccg ttgatcatca
     5701 gccctggggg tgggtacagc gaaggtatcg ccgtgttctt caagtcggcc aacctgagct
     5761 tctgggggcc gtggatgtgg aacggcgtta ccggcgcgcc actggtgctt gggatgcagc
     5821 agacctacgc gggtaccacc tgggatggct acctgcccgc cgctgcctcc acccgccccg
     5881 gtccggtcaa ccaggatacg ctggcgggcc agtaccggtt cttcgacgcg atgggcaacg
     5941 ttatcaactt ccccaacgcc acctcgcgct cggtctggat gacgcagttc caggtgctcg
     6001 ccaccgggcg ggggctgagc ctgttctcgg tccacttccc gccaaaggtg gcccagtccc
     6061 gccaggcttt cggccagttg gcccgcgtgc ccgaaatcac cagcgcactc gtggccgagg
     6121 atagggttgt gctcggcgat ttcaacatca acgcgctcaa cggctgggaa gcaggcgtgt
     6181 tccagcacct gacgggcggg ccgggggtga tagggggagt gcccgtctcg cccatcaact
     6241 atactcagca attcgctgcc gcgaccagca tcaagtcggt caagcaggca tcgaccaccg
     6301 gcgcggcccc ctactacagc tatgcgaagc tgtccagcgc gggggcgctc atggctatcg
     6361 acaacatgtt cgtcgcgcgg gcgggcggtg cggcgggggc ggcgggcaac gctcaagtcg
     6421 ccaaccgcgt aattggcacc gctgccgcgc cagtggctta tacgatggac atggtcagca
     6481 gcatccccgc gattattggt ggtctgccgc cgggcggggc gcgaaagcgg cgactgaaca
     6541 ccctggtcaa ttacggccat atcggcggcg gcagaggaag cagcgaccac atgccggtcg
     6601 ttatcgacct tccctgagag aggactagag cgcaacgatg gctaccaaca cacttagcga
     6661 gctcgcggcg tacctgcaag cccaggccca gcagaccacc ggcgtgacgc tcgattccac
     6721 ctttctcgac acgcgggtgc aaggacttat cgcggacgac ctcctgctgg cggggaaggc
     6781 tatcacgctg gcgcaggtca cccccaagaa cgtcttcgtg agcggcaaca cgctgtacat
     6841 cgtgaatgcc cagacgcccg cctccggggc gctgctgcac ctgaagagcg tacgagccaa
     6901 cgtcgccatc cagcaaacga cgctcaacaa ccccaccggc tacgacctgg tgctgcaact
     6961 cgtgctgccg ggcgagtgga acttcggcgt cagcttcccc gacctcgccc tgctcgaatc
     7021 gcccggctac ctcgacctcg acaccggccc ctacttctac ttttcgtcct tcgaggccgc
     7081 ccagccgctg cccagctttc cggccccccc gaagtcgcag gaactagcgc tggcggaacc
     7141 caccgaagcc gccggcaaca atatcctgct taccggcctg aactactatt ccacggtaaa
     7201 gatcgggggc atcttcgggg tgctcgcgac catcatccac gagaacgacc cgctacctat
     7261 gtacggcctg gttacgtcca cgtcgggcga gacgaatttc acgctcaagg cgtacctctc
     7321 gcagacaagc gtgggcattg gtttcatcag cgcaagctca cccttcctgg gcgtgggcgt
     7381 ctactatccc cctgccgagg cgggcgacga ctcggcggga ggggagcaag aggcgcgcgc
     7441 cttggcgttg ctggacgagg acgaagagga cgcagagggt gacgacgagt cgtacccgat
     7501 gctgctctac tacctgggct gcaccatcac catcacctca caggacggcg agaaactgcc
     7561 gttcgagata gacgtatcta tcccggacct gcaatcgcag caagtggcgg tgtcgattgt
     7621 gtcggaccag agcaagccgc tgaacctgct aaatcttgcg tctgtgctga tgaacagcac
     7681 cgacacggcc atcagcacgg cagtaaagcc gctggaccag tacctcggca ccatcacgct
     7741 ggagcagttc acggcgattt tccagccttc ggccaccacg ccgctgcgct ctatacaggt
     7801 gcagatcggc accgacccgc cctgggatac ccacatcccc agccccagcg atagcagcaa
     7861 cacgctcaag ctcgacctta gcctctcctg ggctatcatg ttcggcccgg ctacccccac
     7921 gtggtccgtc agcttcgacg ccctgctgat attcgacccc aagctgtcgt tcgaagtgac
     7981 cgtgacggtg ccgaacctga tgatcacggg ggccgagcac ggcacggtca cgctctccct
     8041 tagcgacctc aatacgatct tccacacgaa cctgccgata ccggacgacc tgctgactat
     8101 gtccttcacc gacttcatga tcggtatgga cgtgaacaac aagaaataca ccgtcaccgg
     8161 caccatcaac gggcagttcg ccctcttcgg cactcccatc ctgggcttga aggacatggt
     8221 cgtcggcctg gccctggaca cgaaggcttc gacctacacg ctgaacctgg acggcatcgt
     8281 ggcgcttggg tccatcgagg tgcagacgag cgccactatc agcaacgccg cgaacaccga
     8341 caccgtgttc agcatgcacc tggtgaacga gacggtcggc tcgatgctga accacctggt
     8401 gcacctagtc gatcccgatt acgacatcag ctttggcgag ccgtggaaca agctacttga
     8461 cataagcctc gacgcgctgg tgctcgcggt gaacgtcacc aagggcacgg tgagcctgac
     8521 ctacaacgcc actatagacc tgggcttcat ggagattacc ggcgttacgc tgacctacac
     8581 caaggggacc acggctgcca gctcggtgca gatttcgttg gacggcacct tcctgggcca
     8641 gtcgttcggc gggaattcgg gcaagccggg cctcggctgg gacgctatca acgacaaccc
     8701 accggcggtg ccggggcagg gtagctcgct gttcgacctg cggtacgcgg gcctggggca
     8761 gcacatcgcc ttcaccggca tcgatctgaa cacggtggaa gacgtgatga aggcgctcaa
     8821 ggcgtcggtg ctgccgagcc agtcgggcaa gctgccgccc ttcggccaga acagcctcgc
     8881 cttcagccag cagagcaact ggctgatagg tgccgagttc acggtaatgg gcacggtgtc
     8941 tatcagcgcc atcttcaacg accccaacct ctacggtatc ctgatcgcgc tctcaggaga
     9001 gaaggccaag agcttcgccg ggcttagctt cgagatcctc taccacaaag taaccgacac
     9061 gataggcgtc taccacatcg agctaaagct gcccgacgcc atgcgccacc tcgaatttgg
     9121 tgaggtgtcc atcacgctgc ctatcgtgat actggacatc tacactaacg gcaacttcag
     9181 ggtcaatttt ggcttcccga aggggctgga tttctccaac tcgtttgcaa tccaggtatt
     9241 tcccttcgtg ggctacggcg gcttctattt cgcgctgctg aacggcgcaa cctccacacg
     9301 ggtgcccaag atcatcaacg gcaacttctc gcccgtgata gaattcggca ttgccctttc
     9361 gctgggcgta ggcaagacgg tggacgaggg tatcctgtcg ggcggcatca cggtgacggt
     9421 aatcggtatt ttgcaagggg tcctgggctg gttcaacccg accgatcctt cacccaaaga
     9481 gacctactac tggatacagg gcaccatcgc catcgtcggc aagctctacg ccactattaa
     9541 cttcggcatc atctccgcaa gccttgacgt tacggcctat gccagcgtca cgctgaccat
     9601 cgagtcgcac cagccgatct acatcgcgat cagcgcgggg gtgtccgtgc gggtcagcgt
     9661 gaagatcatc ttcttcacga ttcacctgag cttcagcgcc catatcaacg cctcctttac
     9721 gattggctcg gcaagcccca ccccctggaa gctcgccagc ggtagctcgg tatcgagcaa
     9781 cgggcagctg cgccagctac gcggccagca gaccctgcac tcgaccctgc cgctccatgc
     9841 cggttactac agggcgctgc gccgctcgct tatcggcacg cccgctccgc tctccaactg
     9901 gcccgcggtg ctggtgctgc aatcgctgtc ggccattgac ccggccgcga atagtggcgc
     9961 gtcacgcaca ggcggcacca cgaccatcag cacgaccgcc gcgcacgact acgtggtcgg
    10021 ggaccagata aaggtcgcgg gggtggcgga cggctcgttc aacggcacct tcggcgtggc
    10081 ctcggtaact agcaccacgt ttacctacgc acaggccg
//
LOCUS       DASRWZ010000061         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_758555, whole genome shotgun sequence.
ACCESSION   DASRWZ010000061 DASRWZ010000000
VERSION     DASRWZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758555"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VFH60_00860"
     CDS             <1..870
                     /locus_tag="VFH60_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HET6312358.1"
                     /translation="TSFDVIVWQRSETFFLGRYKGLEEIAFYGMAYNIVAIVVGTVPG
                     AFGRVLMPALSVRFGLDDHDSLQALYSTATRYLALLTLPLCAGGIALSTEAVRVMFGL
                     EFAPAGDVMKVLFVGAGIGAIAAAGSALYIALGQTTIAAAWGLPLAVINVLLALVLVP
                     AFGAMGAAVANSLSQIGGVVAGVVYLTHFRGFRLPLRQLARLLLASVPVGLVAYGVVQ
                     LLGGLAGLLAGVLAGAMIYILALILTRALSPRDFDLLEELAGMFPGAIAGVLRRFVGG
                     VRRFSARPADLHT"
     gene            913..1896
                     /locus_tag="VFH60_00865"
     CDS             913..1896
                     /locus_tag="VFH60_00865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6312359.1"
                     /translation="MVASGWQPLVSVIVPTYNREAVLCQTLATLFEQDYPNYEIIVVD
                     QTPQHRDETYRFLEENKQKLRHIKVDKPSLPRARNIGLRHARGELVLFLDDDIIPVRD
                     LVSKHVRGYADDNAVCGVAGQVVPPEGGLVDEGKVGRIDERARLTWNFNSTVAADTMW
                     GPGGNSSFRRKLVEAAGLYEPAFAGTSVAEELDLCVRLYRLGYHMKFEPGASILHLAV
                     SDGGCENRHIGAWWYYWYAHNMALLALRHRSLLNMLLVPPTQARALIRMKGGLRGLPL
                     LAIWLPAQFHALVSYFRSRSSLKGIPSGVRSRGREKRPLQASKSERPNSDK"
     gene            1975..3126
                     /locus_tag="VFH60_00870"
     CDS             1975..3126
                     /locus_tag="VFH60_00870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6312360.1"
                     /translation="MKVGIYYPFEPYVGGGERYTFALAEHLGRAHSVELLTAGLDRIP
                     ALASSLGYDLSRVQLRPRTGYISPVEKAVGSRRYDLFVCISNHAMPPVASLGKRGLLL
                     VQFPFPTRSGDWLRSGLLGPLMLRSYRLALVYSEFVAKWLKLRSPRPIDIRVLAPPVE
                     PVGADPDAPRGRIILGVGRFFEGQHSKRHDVLVEAFRGLVDGGLRGWELHLAGSVRPE
                     PEHQAYLARVRAFAEGYPVVIHTDMAQADLHALYRRASVFWHATGYGTDEQVHPEAME
                     HFGVVTVEAMSAGCVPVVIARGGQPEILQHGVSGFLWSTLDELKALTLQYAASDEAVK
                     RAYRTEAVKASQQYSPRNFQANLDDIVGEVMGQRGLADEHAGSKQRTSG"
     gene            3150..>3649
                     /locus_tag="VFH60_00875"
     CDS             3150..>3649
                     /locus_tag="VFH60_00875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6312361.1"
                     /translation="MNVLYLDATRSLYGASRMLLTLLERADSLGVRPYVVLANDVEDD
                     MRFSRELDRLNVRYWEHPVTVLRRQKYLNPRGVLHLSVSLVKSVGFLRRLIRRYRIDL
                     VQTNTSTILSGAVAARLEGVPHVWHVHELFKRVEGRVLTRLIHARSWRAVVPSEAVAT
                     NLAQSYP"
BASE COUNT          614 a         1101 c         1194 g          740 t
ORIGIN      
        1 accagcttcg acgttatcgt gtggcagcgc tctgagacgt tcttcctggg ccgctacaag
       61 ggcctggagg aaatagcctt ttacggtatg gcatacaaca tagtcgcgat tgtcgtgggc
      121 acggtgccgg gtgcctttgg ccgcgtcctc atgcccgctc tatcggtgcg cttcgggctg
      181 gatgaccacg attcgctgca ggccctctat tctacagcca ccaggtacct ggcgctgctc
      241 acccttccct tgtgcgccgg tgggatcgcg ctctcgacag aagcagtccg ggtcatgttc
      301 gggcttgagt ttgcacccgc gggtgacgtc atgaaggtgc tctttgtggg agccgggata
      361 ggggcaatag cagccgcggg gtccgccctt tatatcgcgt tgggacaaac gaccatcgcc
      421 gccgcgtggg gtctgcccct ggccgtaatc aacgtgctgc ttgcgctggt gctggtacca
      481 gctttcgggg ctatgggcgc ggcggtagcc aacagcctga gccagatagg cggcgtagtt
      541 gcgggcgtag tgtacctgac ccattttcgc ggtttccggc tgccgttgcg gcagcttgct
      601 cgactcttac tggcgtcagt gcctgttggc ctggtagcat atggtgttgt acagcttctc
      661 gggggcctgg cgggtttgct ggccggcgtg cttgcaggtg caatgatata tatacttgcc
      721 ttgatactca cgcgcgcgct gtcacccagg gacttcgatc tgcttgaaga gttggcaggc
      781 atgttccccg gcgctatcgc cggagtcttg cgacgcttcg tcggcggggt gcggcggttt
      841 agcgcccggc ccgccgatct acacacgtga ttagggattg ctgagaaatg atgaatgatg
      901 tccaggaaca atatggtagc tagcggttgg cagccactag tgtcggtgat agtgcccacg
      961 tacaatcgtg aggcagtact ctgccagacc ctggcgaccc tgttcgagca agactatccg
     1021 aactatgaga tcatagtggt tgaccagaca ccccaacacc gggacgaaac gtatcgcttt
     1081 ctggaagaga acaagcagaa gctccggcac atcaaggtag acaagccctc gctgccccgc
     1141 gcgcgcaaca tagggctgag gcacgcacgg ggagaactgg tcctatttct tgacgacgac
     1201 atcatcccgg tgagagacct ggtgagcaag cacgtccgag ggtacgcaga cgataatgcc
     1261 gtatgtggcg tggcgggcca ggtagtgccc ccggagggcg gccttgtgga cgaaggcaag
     1321 gtcggccgga ttgacgagcg ggcgcgattg acctggaatt tcaactcgac cgtggccgcg
     1381 gatacaatgt ggggaccggg tggcaattct tcctttcgcc gcaagctggt ggaggcagcc
     1441 gggctgtacg aacctgcctt cgcgggaacg tcggtggccg aggaattgga cctgtgcgtg
     1501 cgcttgtaca ggctcggcta ccacatgaaa tttgagcctg gagcttcaat tttgcacctg
     1561 gcggttagcg acgggggctg tgagaaccgg catatcgggg cctggtggta ttattggtac
     1621 gcgcacaaca tggcgctcct ggccctgagg catcgctcgc ttctcaatat gcttctcgtg
     1681 cctcctactc aggcccgcgc cttgattcgc atgaagggtg ggttacgcgg cttgcccctc
     1741 cttgcgatct ggctccccgc ccagttccac gcgctggtat cgtacttccg gtcgcgctcc
     1801 agtctgaaag gcataccgtc cggggtgcgg tcccgcggcc gcgagaagag gccactgcaa
     1861 gccagcaagt cggagcgacc gaacagtgac aagtagcaga ccgggcaggg cgtcccacgg
     1921 gtcaggttca actatgccgc ttcaccctca cactgaagtc ggcgcggacg gccgttgaag
     1981 gtaggcatct actatccatt cgagccttac gtgggcggcg gcgagcgcta cacgtttgcc
     2041 ctggccgagc acctgggccg ggcgcacagc gtggagcttc tgacggcggg gttggaccgc
     2101 ataccggcgt tggcttcctc cctgggttac gacctctccc gcgtgcagct acgcccccgc
     2161 accggctaca tttcgcctgt tgagaaagct gtgggcagcc gccgctacga cctgttcgtc
     2221 tgcattagca accatgccat gccgcccgtg gcgtctctcg gtaagcgggg cctgctgctc
     2281 gtacagtttc ccttccccac ccgcagcggg gactggctgc ggtcgggcct gctgggtccg
     2341 ctcatgctgc gctcttatcg cctggctctc gtatattccg agttcgtggc gaaatggctc
     2401 aagttaaggt ctccccgccc catcgatatc cgcgtgctcg cgccccccgt cgagcctgtg
     2461 ggcgctgacc ctgacgcgcc gcgcggtcgg atcatcctcg gtgtggggcg cttcttcgag
     2521 gggcagcaca gcaagcggca cgacgtgctg gtggaagctt tcaggggcct ggtggatgga
     2581 gggctacggg gctgggagtt gcacctggcg ggaagcgtgc gccctgagcc tgagcaccag
     2641 gcctaccttg ccagggtgcg cgccttcgcc gagggctacc ctgtcgtcat ccataccgat
     2701 atggcacagg ccgacttgca cgcgctctac cgcagggcgt cggtcttctg gcacgcgacc
     2761 ggctatggta ccgacgagca ggtccacccg gaggcgatgg agcacttcgg tgtcgttacc
     2821 gtggaggcca tgtcggcggg ctgcgtgccg gtggtaatcg cgcgcggcgg gcagccggag
     2881 atcttacaac acggggtatc gggcttcctc tggagcacgc ttgacgagtt gaaggccctg
     2941 accttgcagt acgcggcctc ggatgaagcc gtgaagcgag cttaccgtac cgaggcggtc
     3001 aaggccagcc aacaatactc gccacgtaac tttcaggcca acctggacga tattgttggg
     3061 gaggtaatgg ggcagcgcgg ccttgcggat gagcatgcgg gctcaaaaca gcggacttcg
     3121 ggctgaccgt ggaggacccg agcaagccta tgaacgttct ttatctcgat gccacgcgca
     3181 gcctgtacgg tgcgagccgg atgctgctta ccctgctcga acgcgccgac tcgctcggcg
     3241 tgcggcccta cgtggtgctc gccaacgacg ttgaggacga catgcgcttc agccgcgagc
     3301 tagaccggct caacgtgcgg tactgggagc accccgtgac ggtgctgcgc cgccagaagt
     3361 acctgaaccc gcgtggcgtg ttgcacctct ccgtctcttt ggtgaagtcg gtgggctttc
     3421 tgcggcggct cattcgccgc taccgcattg acctggtgca gacgaacacc agcacgatcc
     3481 tttcgggtgc cgtggccgcc cgcctggaag gggtgcccca cgtctggcac gtacacgaac
     3541 tgttcaagcg cgtggagggg cgggtgctta cgcggctcat acacgcgcgg tcgtggcggg
     3601 cggtagtgcc ctccgaagcc gtagccacca acctggcgca gtcataccc
//
LOCUS       DASRWZ010000062         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_210313, whole genome shotgun sequence.
ACCESSION   DASRWZ010000062 DASRWZ010000000
VERSION     DASRWZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210313"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFH60_00880"
     CDS             <1..295
                     /locus_tag="VFH60_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312362.1"
                     /translation="EDEILRGVYPERSRRAQNDTGGAWYHTIGTLQIDPSEKETLPAI
                     VVAHEYAHSLGESLKRAGETAAISLETTQVSTSISVYLFNGVRLPTREVEGAN"
     gene            481..2331
                     /locus_tag="VFH60_00885"
     CDS             481..2331
                     /locus_tag="VFH60_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312363.1"
                     /translation="MGDSGTASAGALYAVPTTSAMIPAGGSATLQVRGMSLQPGSTIS
                     GVPASLAAQPVPPDNVRTALYYGINWGYTETDPQQAVLAVWYAQDGTWHGNNHDTAEQ
                     IHSAAASSPGVPSWSPNGRSALQLAGSGQVRLGDVTLAPSSQSPSVGSGTLTLTNTSD
                     QELLVYLPYGTIFGTGPGSVLVWAVGVATQDQPSPTVEETLPLPTDTVEVPESTPTEE
                     AATATATATTAAAAPPEKKSQATATSTAVPGQEASPTEQPPPVKKGGTTATATATPAP
                     TDTATQEPTATATIQPTATAVPAKPSAPNGSAPLDQPASAPVPRDEPAQSPPSDNAAG
                     VAPAENKPATSKQNSTQPPAAGSDTTANTGADTQAPAPKKAQETTSEQAAPAPARDTE
                     QGSRTEALAPAVTMTTAPRAVETAGGTAGDRAAVPPAVQTSGATPPPPPVQTGNPTVP
                     GSTGVPTRPSVARTGTPSPVPTGTPTAADDVPIRETPQPTATRELVPTVEVPTVAAPT
                     APPAPTQDLQPVPTRLTDVEPTPTPPVPVIVTDPGGGSTDGGQGGVTVGSDGGDAGGG
                     TTPSVNPRTGGGPSPLPLVLSISSVLLVLSGWQLRRLAGRQIPGSRDNSH"
BASE COUNT          494 a          880 c          745 g          367 t
ORIGIN      
        1 ggaggacgag attcttcgcg gagtttaccc tgagcgcagc cgaagggctc agaatgacac
       61 aggcggggct tggtaccaca caataggcac tttgcagata gaccctagtg agaaggagac
      121 gttgcctgct attgtagtag cacacgaata tgcgcacagt ctgggggaga gcctcaagag
      181 agcgggagaa acagcggcta tctcgcttga gacgacccag gtaagcacgt cgatcagcgt
      241 gtatctcttc aatggtgtac gtctccccac cagggaggtc gaaggtgcca actaacccct
      301 ggctgcctag cgtctgcttg aaaataggta cggcaggctg tatactcaca gcgcagctta
      361 cccgttcctg aaaggcagat tctcacattg agcaagcccc ggcgcgcctc tactctactt
      421 cgttattgcc ttgcactttt agctttgctt gccgcctccg tgtcgagcgg cgtcttaaat
      481 ttgggcgact ccggcacggc ctcggcgggg gcgctgtacg ccgtacccac cacctcggct
      541 atgataccgg cgggcggtag cgcgactttg caggtgcgtg gcatgtcgct gcagcctggc
      601 tccaccatct ccggcgtgcc cgccagcctg gcggcccagc ctgtgccccc tgacaacgtt
      661 cgcaccgcgc tctactatgg tattaactgg ggctatacgg agaccgatcc ccaacaggcg
      721 gtgctggccg tctggtacgc ccaggacggc acctggcatg gcaataatca cgacaccgcc
      781 gagcagatac actcggccgc cgcatcctct cccggcgtgc caagctggag ccccaacggc
      841 aggtcggccc tccaacttgc cggtagcggg caggtgcggc tcggtgacgt tacactggca
      901 ccttcatcac aaagtccttc cgtgggcagc ggcaccctta ctttgaccaa cacctcggac
      961 caggagctac tcgtctacct gccctacggc accatcttcg gcaccggacc cggttccgtg
     1021 ctcgtgtggg ccgttggtgt agcgacacag gaccagccct ccccaaccgt cgaggaaacg
     1081 cttcccctgc ctaccgacac ggtggaggtg ccggagagca cgcccactga agaagcggcc
     1141 accgcaacgg ccaccgccac gacggcagca gccgccccgc cagagaagaa gtcgcaagcc
     1201 accgccactt cgaccgcggt gccgggacaa gaggccagcc caactgagca accgcccccg
     1261 gtgaagaagg gcgggacgac tgccacggcc acggcgacac cggccccaac cgataccgcc
     1321 acgcaggagc ctaccgctac ggcgaccata cagcccacgg ccacagccgt accggccaaa
     1381 ccctccgccc ccaacggcag cgcgccactc gaccagcctg ccagcgcgcc cgtgcccagg
     1441 gacgagccag cgcaaagccc tccttcagac aacgcagccg gtgtcgcgcc tgccgagaac
     1501 aagccggcaa ccagcaagca gaacagcacg cagccaccgg cggccgggag cgatacaacc
     1561 gcgaacaccg gcgccgatac gcaggcaccc gcgcccaaga aggcccagga gacgacttca
     1621 gagcaggcgg ccccggcccc ggctcgcgac accgaacagg gcagcaggac cgaggccctc
     1681 gcccccgcgg tcacgatgac cactgctccc agggcggtag agacggccgg aggcactgcc
     1741 ggggacaggg ctgcggtacc gccggccgtg cagacatccg gggccacgcc tccgcctccc
     1801 ccagtgcaga cgggcaatcc caccgtgccg ggcagcactg gagtaccgac caggcccagc
     1861 gttgcgcgga cgggcactcc ctcgccggtt cccacgggca cgcctacagc ggcggacgat
     1921 gtgcccataa gggagacgcc gcaacccaca gccacccgtg agttggtacc cacggtggag
     1981 gtgcccacgg tggcggcccc tacggccccg cccgcgccaa ctcaggacct gcaacccgta
     2041 cccacccgcc tcaccgatgt cgagcctacc cccacgcctc ccgtcccggt gatagtgact
     2101 gacccgggtg gaggaagcac ggacggcggc cagggaggcg tgacggtggg ttccgacggc
     2161 ggggatgccg gtggcggcac caccccctcc gtgaacccac gtaccggtgg cggcccctcg
     2221 cccctacccc tggtgctgtc catctcctcg gtgctgttgg tgctgagcgg ctggcaactg
     2281 cggagactgg ccggaaggca aatccccggc agccgagaca actcccacta agtaggagac
     2341 ggaaccgcca aggggccaag gtagcgccaa gattaaggat tgtggctcaa gcaaaaggac
     2401 cagagtacat atggtgtacc ctggtccttt ttgcactctc taaatatcct aaactttgcg
     2461 tgttctttgc gtctttgcgt ttccgt
//
LOCUS       DASRWZ010000063         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_484514, whole genome shotgun sequence.
ACCESSION   DASRWZ010000063 DASRWZ010000000
VERSION     DASRWZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484514"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1764)
                     /locus_tag="VFH60_00890"
     CDS             complement(<1..1764)
                     /locus_tag="VFH60_00890"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET6312364.1"
                     /translation="MARSKKASRGSSAQRRSRTTTPPSNGRSAAPVEVEQVEPEELEL
                     ARPEPARGTRVVRRRRFNLRRALLIMGIVAVLLVAGLFGYMYWQVQRALPTINGTVAF
                     KGLSAPVTVTRDNFGVPHIVAANEKDLYTAQGYVHAQDRLFQMFLFRTVSQGRLSELL
                     SASEDAQIADKFFRTVGFNRAAHAEVAQLPPEVLAALQAYADGVNEFIRTHGDSLPIE
                     FAILGLPMEEWQPVDTIAFGKFQAWDLSQDWGHELLGADLQKQVGAEQAARLMDTYPA
                     GAPVTVPGANSGRAHPMLEAYNKHVRPWLLNLETEGLGSNNWVVDGTKSATGKPLLAN
                     DPHLGVRIPSIWYQMHLRTTDGKYDIAGFSFASSPGIITGHNQDIAWGVTNIGGDVQD
                     LYIEKHDPQGHPGQYQNGDEWLPLQTVTEIIKVKGAEPITHTVRLTERGPIVSDAFDP
                     RDPLGASLEGPVSMQWTAHTPGHLFEAVYDLQTASNWQEFRAALSKWTVPGQNFVYAD
                     TEGNIGYQMTGEYPVRKKGDGSLPVPGYTGEYGWERVVPFEDLARSYNPPEHFIATAN
                     NKPFSDDSDLPIRGNFAGPWRI"
     gene            complement(1914..1985)
                     /locus_tag="VFH60_00895"
     tRNA            complement(1914..1985)
                     /locus_tag="VFH60_00895"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1951..1953),aa:Thr,seq:cgt)
     gene            complement(2116..2346)
                     /locus_tag="VFH60_00900"
     CDS             complement(2116..2346)
                     /locus_tag="VFH60_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312365.1"
                     /translation="MPTGKVYYKAARRPRTEAGGGPGYEERISWIQGELTFEDRYVRA
                     VAHAEETGTRNKTYDFVIPWGSILYIELDTES"
     gene            complement(2442..3023)
                     /locus_tag="VFH60_00905"
     CDS             complement(2442..3023)
                     /locus_tag="VFH60_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET6312366.1"
                     /translation="MSDTTMSLNGKTREDAWELVQRHVHNDGLRKHMLAVEAAMRAYA
                     REYGADEEYWGTLGLIHDWDWEIHPSAEEHPSKGVEMLLEMGWPRELTRSILSHATYS
                     GVPRTEPQDKALFACDELCGLIMATAMVKPNKTLAEVDARSVVKKMKDKGFARAVNRD
                     DIKQGAEELGRPLEEHVDFVVRALQNVSGSLGL"
     gene            3341..3892
                     /locus_tag="VFH60_00910"
     CDS             3341..3892
                     /locus_tag="VFH60_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312367.1"
                     /translation="MFRSRRLTHSVCALAILASLALAGHGSGGVAQAGDGGVIESMTG
                     SGHFTGSNGEFVSFAHNAVKQADGSVNGHYVINLRSLDAVFKGPITCLEVVGNRAWVG
                     GIAESVTSSNPNLAAFEGQEMWFQVADNGEGANDPPDMTTSIGLTPVGGPPGQAAAYC
                     ANMPPPRTFLVGEKGTIQVRDDQ"
     gene            complement(3919..>4173)
                     /locus_tag="VFH60_00915"
     CDS             complement(3919..>4173)
                     /locus_tag="VFH60_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6312368.1"
                     /translation="PVHLARAFRLHFGETPGQYVRRLRLDWSATQLATTDLSITELAS
                     MAGFADQSHFTRVFKSHTGFTPAQYRQCSSKSGRRGGRLR"
BASE COUNT          738 a         1285 c         1319 g          831 t
ORIGIN      
        1 aatccgccac ggcccggcga agttgcccct gataggcagg tcgctatcgt cgctgaaggg
       61 cttgttgttg gcggtcgcga tgaagtgctc cgggggattg taggagcgtg ccaggtcctc
      121 gaacggcacc acacgttccc agccatactc gcccgtgtag ccgggcacgg gcagactgcc
      181 gtcgcccttc ttgcgcaccg ggtactcgcc cgtcatctgg tagccgatgt tgccctccgt
      241 gtcagcgtac acgaagttct gccccggcac ggtccacttg gagagggcgg cgcggaactc
      301 ctgccagttc gaggcggtct gtaggtcgta aacggcctcg aacaggtgcc ccggcgtgtg
      361 ggcggtccac tgcatcgaca caggcccttc aagcgatgca cccagtgggt ctctagggtc
      421 gaaggcgtcg ctgactatcg gcccgcgctc ggtgaggcgt accgtatgcg tgattggctc
      481 cgcacccttc acctttatga tctcggtgac agtttgcaat ggcagccact cgtcgccgtt
      541 ctggtactgg ccggggtggc cctgcgggtc gtgcttttcg atgtacaggt cctgtacgtc
      601 gccgccgatg ttggtcacgc cccaggcgat gtcctggttg tggcctgtga taataccggg
      661 cgacgaggcg aatgagaagc cggcgatgtc gtacttgccg tcggtggtgc gcaggtgcat
      721 ctggtaccag atggagggaa tgcgcacgcc caggtgcggg tcgttcgcca gcagcggctt
      781 gccggtggcg ctcttggtgc cgtccaccac ccagttgttg gagccaagcc cctccgtctc
      841 caggttgagg agccagggcc ggacgtgctt gttgtacgcc tccagcatgg ggtgcgcacg
      901 gcctgagttc gcgccaggta ccgtgacggg tgcgccggcc gggtaggtgt ccatgagccg
      961 ggccgcctgc tccgcgccta cctgcttctg caagtccgcc cccaacagtt cgtgccccca
     1021 gtcctgcgag aggtcccatg cctggaactt gccgaaggcg atggtgtcca cgggctgcca
     1081 ctcctccatc gggaggccaa gtatggcaaa ctcgatgggc aggctgtcgc cgtgggtgcg
     1141 aatgaactcg ttgacgccgt ccgcataagc ctgcaacgca gccagcacct ccgggggcaa
     1201 ctgggctacc tccgcgtggg ccgcgcggtt gaagcccacg gtgcggaaga acttgtcggc
     1261 gatctgagca tcttcactgg cactcaacag ctcggacagc ctcccctgcg ataccgtcct
     1321 gaaaaggaac atctggaaga gacggtcctg ggcgtggacg tagccctgcg cggtgtacag
     1381 gtccttttcg ttggctgcaa cgatatgggg cacgccaaag ttgtcgcggg ttaccgttac
     1441 gggtgcggag agtcccttga aggccacggt gccgttgatg gtcgggaggg cgcgctgcac
     1501 ctgccagtac atgtagccga aaagccccgc gacgagcagc accgccacta tccccatgat
     1561 gagcagggca cgccgcaggt tgaaacgccg cctcctcact accctggtgc ccctggcagg
     1621 ctccggcctc gccaactcga gctcctccgg ttcgacttgt tcgacttcaa ctggggcggc
     1681 gctccggccg ttgctggggg gtgtggtagt gcgtgacctg cgctgtgccg acgagccgcg
     1741 agaggccttc ttcgagcgtg ccatgttagc tccttggtgc caagactcag tgccgcgcca
     1801 catagggccg ggcacgctgg tgaatatacg cagagcctat tatagcagac cccgcaactg
     1861 ggcgaggcta cttcgggcaa caaaaaaggc aggttcgggt acctgcctgc cggagccgat
     1921 ggtgggattc gaacccacga cccatcgctt acgaagcgat tgctctacca ctgagctacg
     1981 tcggcgagag ctattctggg taacattata cggtgcccgc gcggtggctg tcaagcgcgg
     2041 gccgcacagt gagggagagt gctggcggga ctcttagcac gagcgggcct gcggggaggc
     2101 aacgcagacc ggcagctaac tttcggtgtc cagctcgatg tagagaatgc taccccaggg
     2161 gatgacgaag tcgtacgtct tgttgcgagt gcccgtttct tcggcgtggg cgaccgcccg
     2221 cacgtagcgg tcctcgaacg tcaactcacc ctggatccag gagatgcgct cctcgtaccc
     2281 aggaccgcct cccgcttcgg tacgcggtcg gcgcgccgcc ttgtagtaga ctttacctgt
     2341 tggcatatag ggcctcctga gcatgacctg aagttggtga gcagtgctcg gtagtggcac
     2401 aaaatgttga ccaccaggtg gcaagtggct aaaaattcac cctatagacc gagactacct
     2461 gagacattct gcaaggcgcg taccacgaaa tccacgtgct cctccagcgg ccttcctagc
     2521 tcctcggctc cctgctttat atcatcgcgg tttacggcac gtgcgaagcc cttatctttc
     2581 attttcttga ccaccgaacg cgcgtccacc tctgccaaag tcttgttggg cttgaccata
     2641 gccgtcgcca taatgaggcc gcacaactcg tcgcaggcga acagcgcttt gtcctgcggc
     2701 tctgtgcgcg gcacgccgga gtacgtggcg tgagacagta tggacctcgt tagctcccgc
     2761 ggccagccca tctctagcag catctctacg cccttcgagg ggtgttcctc cgccgacggg
     2821 tgtatctccc agtcccagtc atggatgagg ccgagcgtgc cccagtactc ctcgtccgcg
     2881 ccgtattcgc gcgcgtaggc ccgcatcgcc gcctccacgg ccagcatgtg cttgcgcagg
     2941 ccatcgttgt gcacgtgtcg ctgcacaagc tcccaggcgt cttctctggt ctttccgttc
     3001 aggctcatag tggtgtcgct catttatgta ggtgtctcct ggtgcagagt tagtagcaac
     3061 caatatagca aagttgctag ctcagccgct acttagcgtg cgggcgtcag gtccggggcc
     3121 ggcaggaccg cgcatgttat ccaggttcaa ggcgcaggtt ggctacatgc aagacgcagc
     3181 ggctagcggt ggatattatg caaatagccg gtgtgtttct ctgggcctgc gtatagatgt
     3241 gcgcgtcacg tcacgcgtca gatgtcggtg gaacaggcgg cctcatacgt tacgagatgc
     3301 aatgctcgta caactttccg gcatcgaaag gagcttatac atgttcaggt ccagacgttt
     3361 gacacactcg gtgtgcgccc ttgccatcct cgccagcctt gcacttgcgg ggcatggtag
     3421 cggaggagtc gcgcaagcag gggatggtgg agtcatcgaa tccatgacgg ggagcggcca
     3481 ttttaccggc agcaacgggg agttcgtaag cttcgcccat aacgcggtaa agcaggccga
     3541 tgggagcgtc aacgggcatt acgttatcaa tctcaggtcc ctggacgccg tcttcaaagg
     3601 tccgatcacc tgtctggaag tggtaggcaa ccgcgcctgg gtgggaggaa tagccgagag
     3661 cgttaccagc agcaacccca acctggcagc gtttgaaggg caggagatgt ggttccaggt
     3721 cgcagacaac ggcgaaggcg cgaacgaccc gcccgacatg accacttcca tcggcctgac
     3781 cccggtgggg ggacctccag gccaggctgc cgcctactgc gctaacatgc ccccaccccg
     3841 tactttcctc gtcggggaga agggcaccat ccaggtccgc gacgaccagt aactacccgg
     3901 ccgaggcggg cgcctctctc agcggagccg cccgccgcgc ctcccgctct tgctgctaca
     3961 ttgccggtac tgtgcggggg taaagcccgt gtggctcttg aagacacggg tgaagtgact
     4021 ctggtccgcg aatcctgcca tgcttgcgag ttcggtgata gagaggtccg tggtcgcaag
     4081 ctgggtggcc gaccagtcca gcctgagcct gcgcacgtac tgccccggcg tctcaccgaa
     4141 gtgcaatctg aaagcacgag ccaggtggac agg
//
LOCUS       DASRWZ010000064         4865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_758709, whole genome shotgun sequence.
ACCESSION   DASRWZ010000064 DASRWZ010000000
VERSION     DASRWZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758709"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VFH60_00920"
     CDS             <1..1023
                     /locus_tag="VFH60_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6312369.1"
                     /translation="GRLSRISSVSGGSITAGVLALAWHKLRFTGDRLPVAENFNEEVV
                     LPVRGLADKTIDIWAVLRGLFWGGTTSTHIAGYYNRYLFHGRTLQELPKAGQGPDFVF
                     NASNLQSGVLWQSSREYIGDYMVGWLANPDIGVAQAVAASSAFPPFLSPMVLKFNPKK
                     HQFKPGSFSDLNKPPYTAEVLLTDGGVYDNLGLETAWKYFETVLVSDGGGKMGPEGKP
                     GRDWVRQVLRIRDMTDNQVRALRKRQLIASYEAPAGDVNHRSGAYWATRTDISDYKLS
                     DSLPCPPARTAQLANVQTRLGHLDKTTQERLINWGYAICDAAIRGYVDKTLPAPAGFP
                     YPKSKV"
     gene            1104..1658
                     /locus_tag="VFH60_00925"
     CDS             1104..1658
                     /locus_tag="VFH60_00925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016859912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HET6312370.1"
                     /translation="MEDKRLVRLSKYLSKHLRHTPEALGLTLEPGGWVAVEALLDACD
                     RRNFRITREELGEVVARNNKQRFSFDPTRTHIRANQGHSVEVDLQLEPVEPPAVLYHG
                     TASQNVEAIRREGLKRMSRHHVHLSGDMSTALIVGARHGKPVVFDVDAAAMHRAGHEF
                     YCSANGVWLVDHVPPEYLKVTRKA"
     gene            complement(1742..3100)
                     /locus_tag="VFH60_00930"
     CDS             complement(1742..3100)
                     /locus_tag="VFH60_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HET6312371.1"
                     /translation="MVTGTRDRTAEVVRRAAQPLTGGQTDYDALLEKIGDSRFVLIGE
                     ASHGTHEFYRERAQITKRLIADKGFSAVAVEADWPDAYNINRYVRGLGDGATAIESLS
                     GFTRFPIWMWRNADVLDFVGWLREHNDSLPEGGSKAGFYGLDLYSLYGSIQAVLAYLD
                     KVDPEAAGRARYRYGCFEHYAEDTQAYGYAASFGLSDPCEQEVINQLVELQNKAPVYA
                     SMDGKLAEDEFFFAEQNARLVKNSEEYYRTMFHGRVSSWNLRDSHMVETLEALVEHLD
                     KRNPGSKVVVWAHNSHLGDARATEVGQSGEWNIGQLVRERHGDSAFLIGFTTYTGSVA
                     AADNWDEPPRRKKVNPGLPGSYEALFHEVCQAENTSRFLLTLRDGGQAAEALREPRLE
                     RAIGVIYRPATERLSHYFNCRLSDQFDAVIHIDETRAVEPLDQVAGWSSPEPPDTFPS
                     GL"
     gene            complement(3490..4407)
                     /locus_tag="VFH60_00935"
     CDS             complement(3490..4407)
                     /locus_tag="VFH60_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312372.1"
                     /translation="MGLFSGCVGGPQPGSARAIARAEGYMVDMDFSPDWTAVVVGTSH
                     EGAQVWSVADDKPLYKLEAGNARSVAFSPDGNLLALSWRSKDDQRGAVEVRQAADGEL
                     VRSFSDHGTTYSVAFSRDGKLLAAGGSGDVWLYDVSSGEPRWSLDVPGWLVRFSPDGK
                     HLIVSTDRIALYDVDDGVLSREIGGWSEGAFSPDGRLFASPTAQAGLAVQLWDTATWQ
                     AAARLDKAANAQPPPQVHNLAFSPDGKRLVASTNSGTADLWDLGTGTSLRTFEFAQTL
                     DGGKAAFSPDGRRLAIQNAYDIRIWDLDE"
     gene            complement(4544..>4865)
                     /locus_tag="VFH60_00940"
     CDS             complement(4544..>4865)
                     /locus_tag="VFH60_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312373.1"
                     /translation="KVERSAYAGLPARPNATYVLQAEARSNLQSGRFKAYLVCSSAPG
                     GVVRTGPDAAGAASPNDGAWHALRIGVQCPGEAQQLVVALHNPGLGKVSFRDLRVLEV
                     APSR"
BASE COUNT          884 a         1537 c         1553 g          891 t
ORIGIN      
        1 gggcggctgt cacgaatctc gagcgtgtcg ggcggctcga tcactgcggg ggtgctcgcg
       61 ctcgcgtggc acaagctgcg ttttactggc gaccggctcc cggtggctga gaacttcaac
      121 gaggaggtag tgctgccggt gcgcggcctg gcggacaaga ctatcgacat ctgggctgtc
      181 cttaggggcc tattctgggg cggcacgacg agcacgcaca tcgccggcta ctacaaccgg
      241 tacctgttcc acggccgtac tttgcaagag cttccgaagg cggggcaggg tccagacttc
      301 gtgttcaatg cgtcgaacct ccagtcggga gtgctgtggc agtcgtccag ggagtacata
      361 ggtgactaca tggtgggctg gctggcgaac ccggacatcg gcgtagccca ggcggttgcg
      421 gcctcctccg cgttcccgcc cttcctttca ccgatggtcc tgaagttcaa tccgaagaag
      481 caccagttca agcccggctc cttcagcgac ctgaacaagc caccgtacac ggcggaggtg
      541 ctgctaacag atgggggagt atacgacaac ctggggcttg agactgcctg gaagtatttt
      601 gagacagtgc tggtgagcga cggcgggggg aagatgggac cggaagggaa gccagggcgg
      661 gactgggtga ggcaggtcct gcgcatcaga gacatgaccg acaaccaggt gcgcgccctt
      721 cgtaagaggc aactcatcgc ctcatatgag gcgccagccg gggacgtgaa ccaccgctcc
      781 ggtgcgtatt gggcgacacg cacggatatt tcagactaca aactatccga ttctctccca
      841 tgccccccgg ccaggacggc gcagctagcc aacgtacaga ctcggctggg ccacttagac
      901 aagacaacgc aggaaagact catcaattgg ggctacgcga tctgtgacgc cgcgatacgc
      961 gggtacgttg acaagacgct gcccgcgcct gctgggttcc cctacccgaa gtcgaaggtg
     1021 tagagggccg gggtgcgggc gtgtcgtctt gcgtaaaccg aagcagatgg cgggcggcag
     1081 gttcgctgga atggaacaaa gcactggaag ataagcgcct cgtcagactt agcaagtacc
     1141 tcagcaagca cctgcgccac acccccgaag cgctgggcct cacccttgag ccgggcggct
     1201 gggtcgcggt ggaggcgctg ctcgatgcct gcgacaggcg caacttccgc attaccaggg
     1261 aggagctggg ggaggtagtg gcccgcaaca acaagcagcg cttctccttc gaccctacac
     1321 gcacgcacat ccgtgccaac caggggcata gcgtggaggt agacctgcag ctagagccgg
     1381 tggagccgcc agccgtactc taccacggca ctgccagcca gaacgtcgaa gcgatccggc
     1441 gcgagggcct caaacgcatg tcccgccacc acgttcacct ctcaggggat atgtctacag
     1501 cactgatagt gggcgcgcgg cacggcaagc cggtcgtgtt cgatgtagat gcagccgcta
     1561 tgcaccgggc cgggcatgag ttctactgct cggccaacgg cgtatggctg gtggaccatg
     1621 taccgccgga gtacttgaaa gtaacacgta aagcgtaacg cgtaacacgt aacacgtaac
     1681 acgtaaagcg gcttgtgggg tacgtggtac gtgttacgcg ttacgcttta ctcatcacgc
     1741 cctacaaccc actagggaac gtgtccggcg gctccgggga actccagcct gctacctggt
     1801 ccagcggttc caccgcgcgg gtctcgtcga tgtggattac ggcgtcgaac tggtccgaga
     1861 ggcggcagtt gaagtagtgg ctgaggcgct cggtggccgg gcggtaaatg acgcctatgg
     1921 cccgctccag gcgtggctcg cgtagcgcct cggctgcttg gcccccgtcg cgcagggtta
     1981 gcaggaagcg ggacgtattc tccgcctggc aaacctcgtg gaatagcgcc tcgtagctgc
     2041 cgggcaggcc agggttgacc ttcttgcggc gcggcggctc gtcccagttg tcggcggcgg
     2101 cgacgctgcc cgtgtaggtg gtgaagccga taaggaaggc gctgtcgccg tgccgctcgc
     2161 gcacaagctg gccgatgttc cactcgcccg actgcccgac ttccgtggcc cgcgcgtcgc
     2221 ccaggtgcga gttgtgcgcc cataccacca ctttcgagcc ggggttgcgc ttgtccaggt
     2281 gctctaccag tgcctccagc gtctcgacca tgtggctgtc gcgcaggttc caggaggaca
     2341 cgcgcccgtg gaacatcgtg cggtagtact cctccgagtt cttcaccagg cgggcgttct
     2401 gctcggcgaa gaagaactcg tcctcggcca gcttgccgtc catgctggcg tacaccgggg
     2461 ccttgttctg caactccacg agctggttga tgacctcctg ctcgcatggg tcggaaagcc
     2521 cgaagctggc ggcgtagcca taggcctggg tgtcctcggc gtagtgctcg aagcagccgt
     2581 agcggtagcg ggcgcggccc gccgcctccg ggtctacttt gtccaggtag gccagcaccg
     2641 cctgtatgga gccgtaaaga ctgtacaggt cgaggccgta gaagcccgcc ttgctaccgc
     2701 cttcggggag gctgtcgttg tgctcacgca gccagcccac gaagtccaat acgtcggcgt
     2761 tgcgccacat ccagataggg aagcgcgtga agcccgaaag cgactcgatg gcggtagcgc
     2821 catcgcccag accgcgcacg tagcggttga tgttgtaagc gtccggccag tcggcctcca
     2881 cagccaccgc gctgaagcct ttgtccgcga tgaggcgctt ggtaatttgc gcccgctccc
     2941 ggtagaactc gtgcgtgccg tgcgacgcct cgccaattag cacgaaacgc gaatcgccga
     3001 tcttttccaa caaggcgtca tagtcggtct gcccgcccgt gagcggctgc gctgcccgcc
     3061 tgacgacctc tgccgtgcgg tcccgcgtac ctgtaaccat aacccttacc tcctccttct
     3121 actacggcat atggtcaagc aaatcgcagg ccaaacatag actgtgcgga ccggcagtgg
     3181 gacaagtggt agcttggaat agacggaacc gtcgagcctg ccctgagcgt agtcgaaggg
     3241 acgccaagca cgccaagagc gcgccaagat aatttgagta cggttcaccg cagagacgca
     3301 gagacgcaga gttacgcgga gaggagttga gggtgtacaa acatgggcag cagcatacag
     3361 taagtagatg ctgctcttta cctcttacca aacaatccta gtctctgcgt agcctctgcg
     3421 ccctctgcgc cctctgcgcc tctacggtga ataacctcta tcttcttggc gtccgtggcg
     3481 gtttcgtcgt tactcgtcca ggtcccagat gcggatgtcg tacgcgttct ggatggcgag
     3541 gcgtctgccg tcgggtgaga aggctgcctt gccgccatcg agggtttggg cgaactcgaa
     3601 ggtgcgtagg ctcgtgcccg tgcccaggtc ccacaggtcc gccgtgccgc tgttggtgct
     3661 tgccaccagg cgtttgccgt ccggcgagaa tgccaggttg tggacctgcg gcgggggctg
     3721 agcattggcc gccttgtcga gtcgggcggc cgcctgccag gtagccgtgt cccatagctg
     3781 caccgcgagt ccggcctgcg cggtgggaga ggcgaacagc cggccatcgg gcgagaaggc
     3841 cccttcggac cagccaccta tctctcgcga gagcacaccg tcgtccacgt cgtatagggc
     3901 tatgcggtcg gtagagacta taaggtgttt gccatcgggg gagaaccgca ccaaccagcc
     3961 cggcacgtcg aggctccatc gcggctcgcc gctcgacacg tcgtacagcc acacgtcgcc
     4021 gctgccaccg gccgcgagca gtttgccatc ccgcgagaag gccacgctgt aggtggtgcc
     4081 gtggtcggag aatgaccgga ccaactcgcc gtccgccgcc tgcctcacct ccaccgcgcc
     4141 ccgttggtcg tctttggacc gccacgagag ggccagcaaa ttaccatcgg gcgagaaagc
     4201 cacgctgcgc gcattgcctg cctcaagctt gtatagcggc ttgtcgtctg ctacgctcca
     4261 gacctgcgcc ccctcgtggc ttgtgcctac cactaccgcc gtccaatccg gcgagaagtc
     4321 catgtccacc atgtagccct cggcccttgc aatcgctcgc gcggagccgg gctgagggcc
     4381 gccaacacag ccggaaaaca gacccaaaac taacactcct atcaaggcgg tcgcgagcat
     4441 tctgctgata ctcgcctgga cgacccggct cccgttcatc tgcttcttcc aatcgtgagg
     4501 tggattattt tttgcctggc tcagccagcg cggcacgatg cggtcaccgg ctgggagcta
     4561 cttccagcac ccgcaggtcg cggaagctga ctttgcccag gccagggttg tgcagggcga
     4621 ctactaactg ctgggcctcc ccgggacact gtacgcctat gcgtagagcg tgccacgctc
     4681 catcgttggg ggaggctgcg ccagccgcgt caggccccgt gcgcaccact ccgccgggtg
     4741 ccgagctgca caccaggtac gccttgaatc ggcccgattg caggttcgac cgcgcctccg
     4801 cttgcagcac gtatgtcgcg ttggggcgtg cggggaggcc ggcgtaggcg ctgcgctcta
     4861 cctta
//
LOCUS       DASRWZ010000065         6970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_827530, whole genome shotgun sequence.
ACCESSION   DASRWZ010000065 DASRWZ010000000
VERSION     DASRWZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827530"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..2589
                     /locus_tag="VFH60_00945"
     CDS             241..2589
                     /locus_tag="VFH60_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6312374.1"
                     /translation="MFISLRKAYRDLTRRRLRSFLTIIGIIIGVAGIVAITSTSKNMT
                     AAQASAYNNNSQQDMRWWVGISPDNLMAAVNQVPNVETSELRAQYFTKWYATGAWRDI
                     LFNGIKDFDGMRVNRIDLVEGRWPRRGEVVLEASVRDVAPVQIGDEVVYRAGPGNESR
                     HLRVVGFAKSPSYPAASIIGTSVGYTLNTAVRKMYGQDGVNQLLVRLKDFSPGVRTDT
                     KEEIERAFAKRNVNYGSYWERNPDDYVGKRQLDALVTLMTVFSIVGLVISSFLVANTL
                     AAVISEQMSEIGAMKAIGASSGKVISIYVIAGIVYGVVGTGLGLILGVFGGYALLLYL
                     GNLFNLTIQGIAVDPLDIVQGVAVGVGVTTLAAFLPAWRGTAITVRQALDNYGISATY
                     GRGWLDRLVQRLSQLPRVPAMSVRNLARRKGRNVVTVGAIGLAAAAFLAAQSTSDSVN
                     ASIESAYNVYASDAWVWFNEPVGSGFANTLRSMPEVVEVEAWASTSAAVDDLRVTMWG
                     IPADTTLYVYPLVKGRWYTDAEPDVAVISSRFAESRNYQPGDRLELRVGGENAWLTVV
                     GTVNDNAQGLQSSARGKVFVPVATASRLMNRLGAADFFAVRFDRSDGPYVEQTLARLE
                     RKYHTLSPGMLAAYADKASSLEASKILSILLYAMTVIVGTIGGIGIANTLTLNVLERR
                     REIGIMRSLGGRNSHLVQVFMTEALMMGGAGFVLGIALGYPLALLLVWLMSTVLFPLD
                     FVFPANMVLNALVFTILLTMVASVGPALGAARLKVSQALRYE"
     gene            3063..5501
                     /locus_tag="VFH60_00950"
     CDS             3063..5501
                     /locus_tag="VFH60_00950"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET6312375.1"
                     /translation="MADTLKKPITPLTDGHTNGAEHTDADDTLVAAPILPNPPAAVED
                     MPMLAHKRARWRLAIPIVLIIALAIGTGWFFFRPAKSAPTATVRRGTIISTVETTGKL
                     EAERSAKLSFKASGRVERVLVKEGATVKAGDVLAELDTATLQRQLSEAKTQLEISKLQ
                     LQQAKEGAQPEDIAAATADLNAAIARLNQTRAGGRAEDIAAAQAQLNQAQARLDAVKK
                     GATPEDIAQAEATLRQAKAKLEAVKQPATPEQIAQAEATLNQAKARLAAVKQPATPQD
                     IAQAEAQVREAQAKLDALKAGATPEEIAAAQTGVDQAIANLNKVKAPATAQDIAAAQS
                     RVEQAKANRDQVAATAGNSKEQARISMEQASNALQNAQDAYGKIKDENDNTDPKDLTN
                     DQKQAEEKAFRDVQDAQGRLEQARLNYETARQNEIAQLNSANAQVDEAQAAFDKLKAG
                     PTQQDIDVAQAGVDAAVARLNQVKAGPRPEDVTAAQAGLDQAQSRLDALKAGGTPNDV
                     AAAQAEVDRAQASLDALKAGGTPNDIAAAQADVDKAQQALNKLKAGPTAEELREAEEG
                     VVQARANLDKVKKGATQEEINEAQSRVDAAQAALDKVQAGPTNTDIAILEQQINLAQI
                     SVDNASAQVADARLASPIDGTVLALDLEVGETVGGLQQVATVADTTSLRIKADIDEID
                     VGRVSPGQVVTVTLDAYPGVKMPGKIESLAPGATLKQGSTVYQATISFTTTEGVTARE
                     GMASNVDITAQRKDNVLLLPNRAFETVGTRDYVTITERDGTARKVEVETGLSNATDTE
                     VVSGLEEGQVVTLR"
     gene            5513..6349
                     /locus_tag="VFH60_00955"
     CDS             5513..6349
                     /locus_tag="VFH60_00955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6312376.1"
                     /translation="MSSKYDPAYIRDYFERWGEREWQRLDVGPADRVSFHLHRWHLER
                     YIKPGNHVLEVGAGPGRFTIELAKLGAKIIVGDISPVQLELNRRYVQEAGHEQSVAAR
                     EVLDIVDLSRFPPESYDAVVCYGGPLSYVFDRADDALSEMLRVTKPGGFLLLSVMSLL
                     GSTRALLPGVLAVSRQYGLDLVRKVNATGDLHGETAVSEHLCHMFRWSELEALLKRHA
                     GTIVAASASNFLSPGHEEALREVESDPVAWQVFLQWEVDFCREPGALDGGTHIIAVMQ
                     RG"
     gene            6360..>6970
                     /locus_tag="VFH60_00960"
     CDS             6360..>6970
                     /locus_tag="VFH60_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6312377.1"
                     /translation="MATQESTISARGGLFGLSRFHLRLLWVSGIGWMFDAMDIGLLTF
                     LLPTLIKDMGLTGQQPGLVVAFTFIGMFGGAALAGSLSDRYGRKAVFQWTLILYAIGT
                     GLSAFAWDLPSLLVFRLIAGLGLGGELPVASTLVSEWSPPKVRGRMLVLLESFWAYGW
                     TIAALIAFLLVPLFGGDTGTGWRVAFAIGALPALYVLYTRRALP"
BASE COUNT         1438 a         2243 c         2144 g         1145 t
ORIGIN      
        1 aacggggcag cgtttccagg acgtgcctcc ttccaatcct ttttatgtat ggatagagcg
       61 cctggcttcg cggggtgcta tgagtggtta tcagtgcgga ggcccgggcg agccgtgcgt
      121 acgccctgag attcgccctt acttccggtg gggtgctggc gtaaccaggg gccagtccgt
      181 gaagatagta gccaacacct tcttccctgg ctgtgacata tctacggaca ggtaagcccc
      241 atgttcatct ccctccgcaa agcctaccga gacctaacac gccgccgcct gcggtcgttc
      301 ctgaccatca tcggcatcat cataggcgtg gcgggcatcg tcgccatcac ctctacctcc
      361 aagaacatga ccgcggccca ggcgtccgcc tacaacaaca attcccagca ggacatgcgc
      421 tggtgggtgg gcatctcccc cgataacctc atggccgccg taaaccaggt gcccaacgtc
      481 gaaacgagcg aactgcgcgc acaatacttc acaaagtggt acgcgacggg tgcctggcgc
      541 gacatcctct tcaacggcat caaggacttc gacggcatgc gcgtgaaccg catagacctg
      601 gtggaagggc gctggcccag gcgcggggag gtggtgttgg aggcgtcggt gagggatgtc
      661 gcgcccgtgc agataggcga cgaggtagtc taccgcgcgg ggcctggcaa cgagtcgcgc
      721 cacctgcgcg tggtgggttt cgccaagtcc ccttcatacc ccgcggccag catcataggc
      781 acctccgtgg gctacacgct caacaccgca gtgcgtaaga tgtacgggca ggacggcgtc
      841 aaccagctac ttgtgcgcct caaggacttc tcccccggcg tgcgcaccga caccaaagag
      901 gagatagagc gcgctttcgc caagcgcaac gtcaactacg gctcctactg ggagcgcaac
      961 cccgacgact acgtaggcaa gcggcagcta gacgcgctcg ttaccctcat gacggtcttc
     1021 tctatcgtgg gcctggtcat ctcgtccttc ctcgtggcga acaccctggc cgccgtcatc
     1081 tccgagcaga tgagcgagat aggcgcgatg aaagctatcg gggcgagcag cggcaaggtg
     1141 ataagcatct acgtcatagc gggcatcgtc tacggcgtgg tgggcacggg cctgggcctc
     1201 atcctgggcg tgttcggcgg ctatgccctc ctcctctacc tgggcaacct attcaacctc
     1261 accatccagg gcatcgccgt tgacccgtta gacatagtgc agggcgtcgc ggtgggcgtc
     1321 ggcgtgacca cgctggcggc cttcctccct gcatggcgcg gcactgctat caccgtccgc
     1381 caggccctcg acaactacgg catcagcgcc acctacgggc gcggctggct cgaccgcctt
     1441 gtgcagaggc taagccagct gccgcgcgtg cctgccatgt cggtgcgcaa cctcgcccgc
     1501 cgcaaagggc gcaacgtggt gacggtcggg gcgattgggc tggcagccgc cgccttcctc
     1561 gctgcccaga gcacctccga ctcggtcaac gcctctatcg agagcgcgta caacgtctac
     1621 gcctccgatg catgggtgtg gttcaacgag cctgtgggga gcgggttcgc caataccctg
     1681 cgctccatgc ccgaagtggt ggaggtggag gcgtgggcgt ctacttctgc ggcggtggac
     1741 gatctgcgcg tgactatgtg gggcatccct gccgacacta ccctctacgt ctacccactc
     1801 gtgaaggggc gttggtacac cgacgccgag ccggacgtag cggtcatatc ttcccgcttt
     1861 gccgagtcgc gcaactacca gccgggcgac aggctggaac tgcgcgtggg gggcgagaac
     1921 gcctggctga ccgtagtggg cacggtgaac gataacgcgc agggcctgca atcgtcggcg
     1981 aggggcaagg tattcgtgcc ggtcgccacc gcttcccgcc tgatgaaccg actcggagcc
     2041 gccgacttct tcgctgtgcg cttcgacagg tctgacggcc catacgtgga gcagaccctc
     2101 gcccgccttg agcgaaaata ccacaccctc tccccaggca tgctagccgc ctacgccgat
     2161 aaagcgagca gcctggaagc cagcaagata ctcagcatcc tcctctacgc gatgaccgtg
     2221 atagtgggca ccatcggcgg cataggcatc gccaacaccc tcaccctaaa cgtgcttgag
     2281 cgccgccgcg agataggcat catgcgctcc ctcggcgggc gcaactccca tctggtacaa
     2341 gtcttcatga cagaagccct gatgatgggc ggcgcgggct tcgtcctggg catcgcgcta
     2401 ggctacccgc tcgccctcct gctagtgtgg ctgatgagca ccgtcctctt ccccctagat
     2461 ttcgtctttc ccgccaacat ggtgctgaac gcgctagtct tcacaatcct attgacgatg
     2521 gtagccagcg taggccccgc cctcggtgcc gcccgcctca aggtgagcca ggcgttgcgg
     2581 tacgagtgag gggcgaggtt cgcggggcgg ggtgcgaggg gtcaggggtc agggattagg
     2641 tttgataagc cgcagagggc cgaaagatag tagcccctgg cgtgagcctc ggcaaagcca
     2701 taatgtttgt tcatcaagtc cccgtagggg gcgaaagaaa ggaatgttgc aaagtcggga
     2761 ggacaaggga acatcacccc cacatccttg tcattctgag cgaagcgaag aatctcgtcc
     2821 ctcgcgtatt agtttcgggt agcgtagaga cgagagaata aaggcaagaa gatgtacgag
     2881 ggacgagatt cttcgcttcg ctcagaatga caaagggacg tggttcgctt acgtcaggca
     2941 tatatccgat tttgcatcag ccctgactgt atagattagg taattacccg aaccttgcat
     3001 gatatatgta taattaatca caatatacgc tattgatata gaaagaaaag cgggcaaaaa
     3061 agatggcaga caccctcaag aaaccaatca ctcccctaac cgacggccac accaacggcg
     3121 ctgagcatac cgatgccgac gacacgcttg tagccgcacc aatcctaccc aaccctcccg
     3181 ccgccgtcga agatatgccc atgctggccc acaagcgcgc gcgatggcgg ctggctattc
     3241 ccatcgtgct gattatcgcg ctggctatag gcacgggctg gttctttttc aggccggcta
     3301 agagcgcgcc caccgctacc gtgcggcggg gcacgatcat ctccaccgta gagacgacgg
     3361 gcaagctgga agccgagcgg agcgccaagc tctcgttcaa ggcgtcgggg cgcgtggagc
     3421 gggtgctcgt caaagagggt gccaccgtga aggcgggcga cgtgctcgcc gagctagaca
     3481 ccgccacttt gcagcgacaa ttgagtgaag cgaagacgca gctagagatc agcaagctcc
     3541 agcttcagca agctaaggag ggcgcgcaac ccgaagacat tgccgccgcg accgccgacc
     3601 tcaacgcagc cattgcccgc ctcaaccaga cgcgagccgg ggggcgcgcc gaggacatag
     3661 ccgcagccca ggcccagctg aaccaggcgc aggcaaggct cgacgcggtc aagaaaggcg
     3721 cgacacccga agatatagcc caggcggaag ccacgctgcg ccaggcaaag gctaaactcg
     3781 aagcggtcaa gcagcccgcc acgccggagc agatcgccca ggcagaggcc acgctgaacc
     3841 aggcgaaggc caggttagcg gcggtcaagc agcccgccac cccgcaagat atagcccagg
     3901 ccgaggccca ggtgcgcgag gcacaagcca aactggacgc gctcaaggcc ggtgccaccc
     3961 cggaggagat cgccgccgcc caaactggtg tggaccaggc gattgccaac ctcaacaaag
     4021 tcaaggcccc ggccaccgcg caggatatag ccgccgcgca gtccagggtg gagcaggcaa
     4081 aggctaaccg cgaccaggta gccgccaccg cgggcaacag caaggagcag gcccgcatca
     4141 gcatggagca ggcgagtaac gccctccaaa acgctcagga cgcctacggc aaaatcaagg
     4201 acgagaacga caacaccgac cccaaggacc tcactaacga tcagaagcag gcagaggaga
     4261 aagcgttccg cgacgtgcag gacgcgcagg gcaggcttga gcaggcccgc ctcaactacg
     4321 aaacggccag gcagaacgaa atcgcccagc taaactccgc caacgcgcag gtagacgagg
     4381 cgcaggccgc attcgacaag ctgaaagcag gccccaccca gcaggatata gatgtcgcgc
     4441 aggcaggtgt ggacgctgcc gtagccaggc tgaaccaggt gaaagccggg ccgcgccccg
     4501 aagacgtaac ggcggcccag gcgggactcg accaggcgca atccaggctg gacgctctca
     4561 aggcgggcgg cacccccaac gacgtagctg ctgctcaggc cgaagtggac cgcgcccagg
     4621 ccagcctcga cgccctcaaa gcaggcggca cccccaacga catcgcggct gctcaggccg
     4681 acgtagacaa ggcccagcag gctcttaaca agctcaaggc aggccccaca gccgaagagt
     4741 tgcgcgaggc cgaggagggc gtggtccagg cgcgggccaa cctggacaag gtgaagaagg
     4801 gtgctaccca ggaggagata aacgaggcgc aatcgcgggt ggacgcagcc caggccgccc
     4861 tcgacaaggt gcaggcaggc cccacaaaca ccgacatcgc tatccttgag cagcaaatca
     4921 acctggcaca gatttcggtg gacaacgcga gcgcccaggt ggccgatgcc cggcttgcct
     4981 cgcccataga cggcaccgtg ctagccctgg acctggaagt tggcgagacg gtaggggggc
     5041 tgcaacaggt cgcgaccgta gccgacacta cctccttacg tatcaaggcg gacatagacg
     5101 agatagacgt gggccgcgtc agccccggcc aggtcgttac cgtcaccctc gacgcttacc
     5161 caggcgtgaa gatgcccggc aagatcgagt cgctcgcccc aggcgcgacc ctcaagcagg
     5221 gcagcaccgt ctaccaggcg accatcagct tcactacaac cgaaggcgtc accgcccgcg
     5281 agggtatggc ctccaacgtg gacatcaccg cccagcgcaa ggacaacgtg ctcctcctcc
     5341 ccaatcgcgc tttcgagacg gtaggcaccc gcgattacgt cacaatcacc gagcgcgacg
     5401 gcacggcccg caaagtagag gtggagaccg gcctatccaa cgccaccgac accgaagtgg
     5461 tctccggcct cgaagagggg caggtagtca cgctcaggta ataggctagc gcatgagttc
     5521 caaatacgac cccgcataca ttcgtgacta cttcgagcga tggggagagc gcgaatggca
     5581 gaggctcgac gttggccccg ccgatagggt cagtttccac ctccacaggt ggcaccttga
     5641 gcgttacata aagccaggaa accacgtact agaagtgggc gcggggccgg gccggttcac
     5701 catcgagctt gcgaagctgg gggccaagat aatcgtcggc gatatttctc ccgtccagct
     5761 agagctgaac cgccggtacg tccaggaggc aggccacgag caaagcgtgg cggcgcgtga
     5821 agtcctggac atcgttgacc tctctcgctt ccctcctgaa agttatgacg cggtagtctg
     5881 ctacggaggg cctctcagct acgtcttcga ccgcgcggac gatgccttga gtgagatgct
     5941 acgtgtaacg aagccgggcg ggttcctctt attgagcgtc atgtcgctgc tggggtcaac
     6001 cagggctttg ctgccgggag tgcttgccgt cagccgccaa tacggcctcg acctggtgag
     6061 gaaggtcaac gccacgggcg acctgcacgg cgaaactgcc gtttcggagc atctctgcca
     6121 catgttcagg tggtccgaat tggaagccct tctgaagcga cacgcgggca ctatagttgc
     6181 tgcttccgct tccaacttcc tctcgccagg ccacgaagag gcgctacgag aggtcgagag
     6241 cgaccccgta gcgtggcagg tcttcctcca atgggaagtt gacttctgcc gggagccggg
     6301 tgccctggac ggcggcaccc acatcatcgc cgtgatgcag cgcggctgag ggtaagcgca
     6361 tggcgacgca agaatctacc atctcagcgc ggggcgggct gttcggcctg agcagattcc
     6421 acctccgcct cctgtgggtg tcgggtatcg ggtggatgtt cgacgcgatg gacatcggcc
     6481 tgctcacctt cctgctgccc accctcatca aggacatggg gctgaccggc cagcagcccg
     6541 gcctggtggt ggccttcacg ttcatcggca tgttcggcgg ggccgcgctc gcgggtagcc
     6601 tgtctgaccg ctacgggcgc aaagccgtct tccagtggac tctcatcctc tacgcaatcg
     6661 gcaccggcct gagcgccttc gcctgggacc tcccttcgct cctcgtcttc aggctcatag
     6721 ccggcctggg tctcggcggc gagttgcccg tagcctccac gctcgtcagc gagtggtcgc
     6781 cacccaaagt caggggccgc atgctcgtgc tccttgagag tttctgggca tatggctgga
     6841 ccatcgccgc tcttatcgcc ttcttactgg tccccctgtt cggtggagat accggcaccg
     6901 gctggcgcgt ggccttcgca ataggtgcgc tgcccgccct ctacgtgcta tatacccgcc
     6961 gcgccctgcc
//
LOCUS       DASRWZ010000066         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_142207, whole genome shotgun sequence.
ACCESSION   DASRWZ010000066 DASRWZ010000000
VERSION     DASRWZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142207"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..>2443
                     /locus_tag="VFH60_00965"
     CDS             122..>2443
                     /locus_tag="VFH60_00965"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HET6312378.1"
                     /translation="MKNLLGRLMSRPFDELRVIAATWGTLTRDPNPPQNDLAIAVYHT
                     MMDRSAVRGVWEGLSPEERSFVGWLLSQRNMLALADELPAQLDRPPAEVEALLGRVRR
                     AGLIDVDEALVRGSRVVSSGDNLYAWATRSQPEATRRKVVSISTEGAKVLREVIDEAR
                     LYATSTPFEEPFAALLGNLTEEDVQRIATTWKLPEAARYYKSELIGVMGEFLATGQGR
                     ELLLTQLPPSSQALFTYLEGNAGKDTASGARKHFGWDERELRSALIPLVQRALVWDIL
                     AGDRRFLFVPTDLSQNGNSAGGSNGAGKAPPYMQPKLEARPPHSAEARLSYEMAWDLL
                     TLLSTAAQGDLQLTLQDTRITKRVAKKINDSFLQPEDLKAGSDYIDMVVHMAQSLGLL
                     SQTADAENGVQPTLRLTSRADDWARLSFDAQRRRLYGLWQEDRKWSEPATYGTIYWWN
                     SDLTGGRKRLVHHLLDLPVGQWTSLDSFLRKIHMTEPFLIWPQDELVRRFGLRALQGF
                     RSQWFEIEGRIIADMLKTMLHWLGVVEIGRDKPKRFISFRVTAEGRALLDPDLAADSV
                     APPAKTLLVQPNFEVLVLHPDSRVLWKLVRMADLVRHDRVSVYMLNKESVMRAVEAGM
                     SPEEITGFLNTNTGKALPQNVAHSIGDWARMLKRLTIQRVTLIQVDDPAVLDEILASR
                     KTKRMVLLRLSPTTAIASLPDVSEGAREDAWQRLAKELRGAGYVPTLLPEQSGAEERE
                     APARAKPLRRQAADTGATGQEPGRPRARTRTTRT"
BASE COUNT          445 a          870 c          725 g          403 t
ORIGIN      
        1 tctaaacact cagcgacccc cggctatttg acaagccccc cacctttagg tatagtctag
       61 aatcacgctg cacaatgata cgtgcgcgca atcaagaacg ttaggatgcg atttccgaag
      121 tatgaagaac cttcttggca ggctgatgag caggcccttt gatgagcttc gcgttattgc
      181 agccacctgg ggcacgctca ctcgtgaccc taacccaccg cagaacgacc tcgccatagc
      241 ggtctaccac actatgatgg accgctcggc cgtgcgtggc gtgtgggaag gcctctcccc
      301 tgaagagcgc tccttcgtcg gctggctcct cagccagcgc aacatgctgg ccctcgccga
      361 tgagcttccc gcccaactcg accgcccacc cgcagaggtg gaagccctgc ttggccgcgt
      421 ccgccgcgcc gggcttatcg acgtggacga ggccctcgtg cgcggcagcc gcgtcgtgtc
      481 gtccggcgac aacctctacg cctgggctac ccgctcgcag cccgaagcca cccgccgtaa
      541 ggtcgtatcc atctcgaccg agggcgccaa ggtgctgcgc gaggtgatag acgaggcccg
      601 cctctacgcc accagcaccc ctttcgagga acccttcgcc gccctcctgg ggaacctaac
      661 cgaggaagac gtgcaacgga ttgctaccac ctggaagctg cccgaggccg cccgctacta
      721 caagtcggag cttattggcg tgatgggcga gttcctggcg accgggcagg gtcgcgagct
      781 actgctcacg caactccccc cctcctcgca ggccctcttc acctacctgg aaggcaacgc
      841 cggcaaagac actgcgtctg gcgccaggaa gcattttggc tgggacgagc gcgagttgag
      901 gtcggccctg attcccctcg tgcagcgcgc cctcgtgtgg gacatcctgg ccggcgaccg
      961 ccgcttcctg ttcgtgccca ccgacctgtc gcagaacggc aacagtgctg gcggctccaa
     1021 cggcgcgggc aaggcccccc cttacatgca gcccaagctc gaagcccgcc cgccccactc
     1081 cgcggaggct cgcctctcct atgagatggc ctgggacctg ctcaccctcc tctccaccgc
     1141 cgcgcagggc gatttgcagc ttactctgca ggacacccgc atcaccaagc gcgtcgcgaa
     1201 gaagatcaac gactcttttc tgcagcccga agacctcaag gcgggcagcg actatatcga
     1261 tatggtcgtg cacatggccc agtcgctcgg cctgctctcc cagaccgccg acgctgagaa
     1321 cggcgtccag ccgacgctgc ggcttacctc acgcgctgac gactgggcgc ggctctcctt
     1381 cgatgcccag cgcaggcggc tctacggcct ctggcaggag gaccgcaagt ggtctgagcc
     1441 tgctacttac ggcacgatct actggtggaa cagcgacctc accgggggcc gcaaacgcct
     1501 ggtgcaccac ctgctcgacc tgccggtggg ccagtggacc tccctcgata gcttcctccg
     1561 caagatacac atgaccgagc cgttcctcat ctggccgcag gacgagttgg tccgacgctt
     1621 cgggctgagg gccttgcagg gcttccgcag ccagtggttc gagatcgagg gccgcattat
     1681 cgcggacatg ctcaagacga tgctccactg gctgggcgtg gttgaaattg ggcgggacaa
     1741 gccgaagcgc ttcatctcgt tcagggtcac cgctgaaggc cgcgccctgc tcgaccccga
     1801 tttggccgcc gactcagtag ccccgcccgc caagacgctg ctcgtacagc ccaactttga
     1861 ggtgctggtg ctccaccccg atagccgcgt gctgtggaaa ctcgtgcgca tggccgacct
     1921 ggtgcggcac gaccgcgtga gcgtctacat gctgaacaaa gagagcgtca tgcgggcagt
     1981 cgaggcgggt atgtcacctg aggagattac aggcttcctc aacaccaata cgggcaaggc
     2041 gctcccgcag aacgtcgccc actccatcgg cgactgggcg cgcatgctca agcggcttac
     2101 tatccagcgc gtgaccctca tccaggtgga cgaccccgcc gtgctggacg aaattctcgc
     2161 ctctcgcaag accaagcgca tggtgctgct acgcctctcc ccaaccacgg ctatcgccag
     2221 cctacccgac gtgtcggagg gtgcccgcga ggacgcctgg cagaggctcg cgaaagagct
     2281 gagaggcgcg ggctacgtgc ctacccttct ccccgaacag agcggcgctg aggaacgcga
     2341 agccccggcc agggccaagc ccctgcgcag gcaggccgcc gatacgggcg cgacaggcca
     2401 ggagccgggc cgcccccgcg cgcgtacccg caccaccagg acc
//
LOCUS       DASRWZ010000067         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_827593, whole genome shotgun sequence.
ACCESSION   DASRWZ010000067 DASRWZ010000000
VERSION     DASRWZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827593"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..293)
                     /locus_tag="VFH60_00970"
     CDS             complement(72..293)
                     /locus_tag="VFH60_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET6312379.1"
                     /translation="MIDQDLITRDPEIMSGTPVFKGTRVPVRSLFDYLKAGDTLAYFL
                     ESFPSVERKQAEAVLALAAMSRGLSEEVM"
     gene            601..>2614
                     /locus_tag="VFH60_00975"
     CDS             601..>2614
                     /locus_tag="VFH60_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET6312380.1"
                     /translation="MKAVIMAGGEGSRLRPLTIRRPKPMVPVVDRPAIVHIMELLKRH
                     GITEVVITLQYLASTIQEYLGDGSAMGMKVHYTVEESPLGTAGSIRLGEQHLNETFRV
                     ISGDALTDFDLGAAVAFHKQKKSMASIVLKRMPNPLEYGVVIIDEDGRVRQFQEKPSW
                     GEVFSDTVNTGIYILEPEIFKYYEAGQVFDFSSQLFPQMLEKGDPMFGYLADGYWCDV
                     GSIPDYMTASADYLGGQVSVPRMGKETAPGSGIWAEEEVEIARDARVIGPVYLGTGVK
                     INSGAVVRGPAVIRDFTIIDNRATIDRAIIWRNCYIGERAEVRGAILQTQVNVKAQAM
                     IFEGAVIGDGTIIGNGAVIQPNVKIWPSKEVESGATVTSSIIWGAQGRRSLFGRWGVT
                     GLVNVDLTPEFAAKLGAAYGGVLARGTTVTVNRDSHYTPRMIKRALIAGLPSAGVNVL
                     DIRNVPIPVARHYTRSVGAAGGVHVRLSPFDARVVDIKFFDKRGLDLDKATERKIEGL
                     FFREDFRRVYLDDVGRINYAQDVESRYASEYMRHLNVDAITNSSDGYTRIAVDYANSS
                     SSLVLPNLLGRLNADVVAINAVVHEESLYQTPEQFEQAMERLGAITATLKAGFGVRLD
                     TGGERISFVDETGREVDRMRALAVVVDLALAQHAAEHGKGGIAVVPV"
BASE COUNT          530 a          811 c          821 g          452 t
ORIGIN      
        1 accatcccgg tggaggacga gattcttcgc tgcgctcaga atgacaaagg ccgctttggt
       61 taccgtaatt gctacataac ctcttctgac aagcccctgg acatcgcggc caatgccagc
      121 acagcctcgg cctgcttcct ctcaaccgag gggaacgact caaggaagta ggccagtgta
      181 tcccctgcct tcagataatc gaacaacgac ctgacaggca cgcgggtgcc cttgaagacg
      241 ggcgtaccac tcatgatctc agggtcgcgg gttatcaggt cttggtctat catggctcac
      301 atattcaact atcgtattcg catggcataa tctcacgcgt tgcgggcgaa aatccaaaat
      361 gatcctgcat tatagaccag cccacggcaa cgaaccaccc gcaccctaca aaaacccgcc
      421 accctcctgt ataattcacg ataggcgcaa cggcgcgcta cgcgctcccc catttccttg
      481 atttcctgcg ccccttacaa ttttggattt tagattttgg attttagatt agggccgaag
      541 agagccaatc gaaaatccaa aatctaaaat ctaaaatcca aaatccccgg agggcgtctt
      601 atgaaggcag tcatcatggc aggcggcgag ggttctcgcc tgcgtcctct cacgatacgg
      661 cgtcccaagc cgatggtgcc cgtcgtggac cgcccggcta tcgtgcacat catggagttg
      721 ctcaagaggc acggcatcac cgaggtggtc atcaccctgc aatacctcgc ctccacgata
      781 caggagtacc tgggcgacgg ctccgcgatg ggcatgaagg tgcactacac ggtggaggaa
      841 agccccctgg gcaccgcagg ctccatccgc ctgggcgagc agcacctcaa cgagaccttc
      901 agggtcatct ccggcgacgc actgaccgac ttcgacctgg gggccgccgt ggccttccac
      961 aagcagaaga agtcgatggc ctctatcgtc ctcaagcgca tgcccaaccc cctcgaatac
     1021 ggcgtggtca tcatagacga ggatgggcgg gtgcggcagt tccaggagaa gcctagctgg
     1081 ggcgaagtct tctccgacac ggtgaacacc ggcatatata tcctggagcc tgagatcttc
     1141 aagtactacg aggccgggca ggtgttcgac ttcagcagcc agctatttcc acagatgctg
     1201 gagaagggcg accctatgtt cggctacctg gccgacggct actggtgcga cgtcggctcg
     1261 atacccgact acatgaccgc ttcagccgac tacctggggg gccaggtgag cgtaccgcgc
     1321 atgggcaagg agaccgcgcc aggctccggc atctgggccg aggaagaggt ggagatagcc
     1381 cgcgacgccc gcgtaatcgg gccggtctac ctgggcaccg gcgtcaagat caactcgggc
     1441 gcggtagtac gaggcccggc ggtgatccgc gacttcacga tcatcgacaa ccgcgccacg
     1501 atagaccgcg ctatcatctg gcgcaactgc tacatcggcg agcgggccga ggtgcgcggg
     1561 gctatcttgc agacccaggt gaacgtcaag gcccaggcga tgatcttcga gggggcggtg
     1621 attggcgacg gcacgatcat cggcaacggc gcggtgatcc agcccaacgt gaagatctgg
     1681 ccttccaagg aagtggagag cggggctacg gtgacctcct ctatcatctg gggggcgcag
     1741 ggccgccgct cgctctttgg gcgctggggc gtgaccggcc tggtgaacgt ggacctcact
     1801 ccggagttcg cggccaagct gggggcggcg tatggcggcg tgctggctag aggcaccacg
     1861 gtgaccgtga accgcgacag ccactacacc ccgcgcatga tcaagcgcgc cctcatcgcg
     1921 ggcctgccct cggcgggcgt gaacgtgctc gatatacgca atgtgcccat ccccgtggcc
     1981 cggcattaca cccgctcggt gggcgcggcg ggcggcgtgc acgtgaggct gtcgcccttc
     2041 gatgcccgcg tggtggacat caagttcttc gacaagcgcg gcctcgacct ggacaaggcg
     2101 accgagcgca agatcgaggg cctgttcttc cgcgaggact ttcgccgcgt ctacctggac
     2161 gacgtggggc gcatcaacta cgcgcaggac gtggaaagcc gctacgccag cgaatatatg
     2221 cgccacctga acgtggacgc catcaccaac agcagcgacg gctacacccg catcgcggtg
     2281 gactacgcca actcgtcgtc cagcctggtg ctgcccaacc tcctggggag gctcaacgcg
     2341 gacgtggtcg ccatcaacgc ggtggtgcac gaggagtcgc tctaccagac gccggagcag
     2401 ttcgagcagg cgatggagcg gctgggggcc atcacggcca cgctcaaggc ggggttcggg
     2461 gtgaggctcg acacgggcgg cgagcgcatc tctttcgtgg acgagacggg cagggaagtg
     2521 gaccgcatgc gggcgctggc ggtggtagtg gacctggcgc tggcccagca cgcggcggag
     2581 cacggcaagg gcggcatcgc ggtggtgccg gtga
//
LOCUS       DASRWZ010000068         1617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_210679, whole genome shotgun sequence.
ACCESSION   DASRWZ010000068 DASRWZ010000000
VERSION     DASRWZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210679"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFH60_00980"
     CDS             complement(<1..628)
                     /locus_tag="VFH60_00980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6312381.1"
                     /translation="MPLRSIHWLRRLRGRTVRHITVFTLTLLLIEFLDELTFGVREAA
                     WPLIRDDLNLDYSQVGILLGLPNLASTFIEPLICILGDVWRRRVLVLGGGVLFGLALL
                     LTALSQHFLVLLASFILLYPASGAFVSLSQATLMDTDPTRHEQNMARWTFAGSLGVVA
                     GTFSVVAGSWLGVDWRTLFLLLAGLSFLLVMLAWRSPFPRPQQEEEEEA"
     gene            complement(635..>1617)
                     /locus_tag="VFH60_00985"
     CDS             complement(635..>1617)
                     /locus_tag="VFH60_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312382.1"
                     /translation="LPFALLPALPLLRSATSETNSKLKTQNSKLSLRLVVSTLIALGF
                     FVNALGAVVNFDTYINVGYDDETRHWIPYASPIVGHLNLLGQRVSEKASTLRPPERTV
                     VFRGGVSYSEGDKASGALLPRWTTGRAVLELWPPASRGPVSVTLRLNDHRPPQLPRAA
                     VTILVNDNPITVDRAPVPDLPISADYSFLVSGQPARVIIATDTWNPAKLGENERDEEL
                     GVRLESITVREGGHVQPYRLADDLAAPPYYPQPRWYYNPATHFLADLWPVYMLEMGMG
                     RKTMLALGLPIIVLGLLCLGAGCYGLARRGQGPKIGSREIVHNQAGPVEV"
BASE COUNT          307 a          450 c          579 g          281 t
ORIGIN      
        1 gcgcctcttc ctcttcctcc tgctgaggcc gggggaatgg ggagcgccac gccagcatca
       61 cgagtaggaa ggacagcccg gccagcagca ggaagagcgt gcgccagtct acgcccagcc
      121 aggaaccggc gactacggag aaagtgcccg cgaccacgcc cagcgatccc gcgaaggtcc
      181 agcgtgccat gttctgttcg tggcgagtgg ggtcggtgtc catcagggtg gcttgggaga
      241 ggcttacgaa cgcgcctgag gccgggtata ggaggatgaa agaggcgagc agcacgagga
      301 agtgctggct caaagccgtg aggagcagcg ccaggccgaa taggacgccg ccgccaagca
      361 ccagcacccg acgccgccaa acgtcgccca ggatacatat aagcggctct atgaaggtgc
      421 tggcgaggtt gggcagccct agcagtatgc cgacctggga gtagtcgagg ttgaggtcgt
      481 cccgtatgag gggccaggcg gcttcgcgca ctccgaaggt caattcgtca aggaactcta
      541 tgaggagcag cgtcagggtg aagacggtga tatgccggac tgtgcgacct cggagccgcc
      601 gcaaccagtg tatggagcgc agaggcatac agcgttatac ctctactggc cccgcctggt
      661 tgtggacgat ctctcggctg ccgatctttg gaccctgacc tcttcgcgcc agtccatagc
      721 acccggcacc caggcagagc agccctagca caataatggg caatcccagc gcgagcatcg
      781 tcttgcggcc catgcccatc tcaagcatgt agacgggcca caggtcggcc aggaagtgcg
      841 tggcggggtt gtaataccag cgcggctgtg ggtagtaggg gggcgcggca aggtcgtccg
      901 cgagacggta aggctggaca tggccccctt cgcgcacggt gatgctctcc aacctgacgc
      961 caagctcctc gtcccgctcg ttctcgccca gcttggcggg gttccaggtg tcggtggcga
     1021 ttatcacgcg cgcaggctgc ccgcttacga ggaaggagta gtcggcgctt atcggcaggt
     1081 cgggcacggg cgcacggtct accgttatgg ggttgtcgtt gaccaggatg gtgacggcgg
     1141 cgcgtggcag ctgcgggggg cggtggtcgt tcaggcgcag ggtgacggag acaggtccgc
     1201 gagatgcggg gggccaaagc tctagcacgg ccctgccggt ggtccagcgc ggtaggagtg
     1261 cgccgcttgc tttgtcgccc tcgctgtacg acacgccacc cctgaagacg actgtgcgct
     1321 caggcggtct cagagttgag gctttttcgc tcacacgctg gcccagcagg ttgagatggc
     1381 ctacgatggg cgaggcgtag ggaatccagt ggcgggtctc gtcgtcgtag ccgacgttga
     1441 tgtaagtgtc gaagttgacg acggcgccca gagcgttcac gaagaaaccg agggctatca
     1501 acgtggaaac taccagccgg aggctgagtt ttgagttttg agttttgagt tttgagtttg
     1561 tttcgctcgt agcggagcgg aggaggggga gagcggggag gagggcgaac gggagga
//
LOCUS       DASRWZ010000069         3924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_896226, whole genome shotgun sequence.
ACCESSION   DASRWZ010000069 DASRWZ010000000
VERSION     DASRWZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896226"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VFH60_00990"
     CDS             <1..1572
                     /locus_tag="VFH60_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312383.1"
                     /translation="LVELRAVAEVQPGYRDVTSMQAAAEKQVYRDALLGAVAMRPDAD
                     PPGLYYRAGWDWIWLEGSDEQSLPWGQGTTDHLLYDAPVEGWVPPTRANRRNLGQDGR
                     ELDGRRLMVASFDGGNAAGVDFRELPFKLSDFHFFEWHNEGVWGIRYADAVTPRVGEP
                     GPVRDRINYIQQITYHAFGTMIMRSTNAAYYGMNVALMSYNRNGDDLLLANWTRHDDG
                     TFTTDLYLNSLTNGENRLLYNHDGGFGSASFSDDGKFVFLTTYKPSDDALEDRLHLLI
                     DTTEARPPRVLLQQIGLRTTNEYYMLNELVTDAQLLERSPASGKLFFTYWMSSTNYIS
                     MLDPDGPGLAQPLASVKGLGRIQWTAASTEGPDVVLWGIGTTGGRTAVTPGQQALVVV
                     AIPPSGPPVVAQMDMGELSNITMIAQRGTDLVYLLYGYTREGQLKRAELRSVPLSGAG
                     QGRLESTLLSTYTYPSRGKGRFYFDEDFRFSFGPGMLAYLDGSTLRAVTYDGQTDLAL
                     ESDVAALFRVGVAEP"
     gene            1667..3733
                     /locus_tag="VFH60_00995"
     CDS             1667..3733
                     /locus_tag="VFH60_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312384.1"
                     /translation="MSRYSNRLPALASLALPVLALLAWAGLVMLLAYQARPAYAVSMD
                     RSFDRHYLTDGWHQPEENPLFKFRWMSGYATVRFPAIGRQPLKLVIQMGTQGIDPTPE
                     KKVQVTARGALVAVFDVRPSIERFEVPVSPELVSRATGDLDLLVTMPSFQPPGDRRQL
                     GVVVTGLSLEPDGTGGPVLPPAYHALYVAGIGASLYFMLLLLGIGVRARLALSMVAVL
                     AMGLVYAFVRPAIAFYAGDLLLAAVLALVALAVAQPAVTWLYRRAGYSDFRPRSGEGR
                     LLFGLFGFGLLVGWAGLLYPQSEPHDFLFHLHRFNEVQAGNLFFENYVVSGVGQGFYP
                     PAMYVLLLPFSLLLRDPYNLVLLAPTLFSLSAVFIVFYIAKRYMSGYRHAPILASALY
                     VALPVNLLVIWWAHETNLFGLVVLLAVVAYVLVGYGRIGRPAVWLGLAALTFVLLLSH
                     PGVLVWSVTLLGGTVLALLGLRRFAANGSYRSVLLVAGAFAAAGLLAFVLYYSRYLGS
                     FGQAAVERGGEVDFAETLSSVGDVDRLLDMIRLTLYHGVLGDYGLLPLLLVPVGLWLL
                     FRGRDTVSADEAIPHEASQEERQRFRWVMLVWVASGLLFLLVSLLTALPIRPMLFMWP
                     PTALLAGLSLAAFVEGERAPGRAWTWRKVATLGLLAGLAALSLYLWALANFLDMREPH
                     VYPLVF"
BASE COUNT          607 a         1247 c         1306 g          764 t
ORIGIN      
        1 ctcgtggagt tgcgggcggt ggcagaggtg cagcccggct accgtgatgt gacctctatg
       61 caggcggccg ctgagaagca ggtatacagg gacgccctgc tcggcgccgt cgccatgagg
      121 ccggacgccg acccgcccgg cctctactac agggccggct gggactggat atggctcgag
      181 ggcagcgacg agcagagcct gccctggggg caaggcacaa ccgaccacct tctgtacgat
      241 gcgcccgtag aaggctgggt gccccccacc agggccaacc gccgcaacct cggccaggac
      301 ggcagggagc tggacggccg ccggttgatg gtggcttctt ttgacggcgg gaacgcggcg
      361 ggtgtggact ttcgggaact ccctttcaag ctgagcgact ttcacttctt cgagtggcac
      421 aatgagggtg tgtggggcat ccgctacgcc gacgctgtca cgccccgcgt gggcgagcct
      481 ggcccggtgc gagataggat aaactacatc cagcagatta cctaccacgc gttcggcacc
      541 atgatcatgc gcagcaccaa cgcggcgtac tacggcatga acgtggcgct catgagctac
      601 aaccgcaacg gtgacgacct gctcctggcg aactggacca ggcacgacga cggcacgttc
      661 acgacggacc tctatctcaa ctcgctcacc aacggcgaga acaggctctt gtataaccat
      721 gacggcggct tcggtagcgc gagcttcagc gacgacggca agtttgtttt cttgaccacc
      781 tacaagccgt cggacgatgc ccttgaagac cgccttcacc tcctcatcga taccacagaa
      841 gccaggcccc cacgcgtgct gttgcagcag atagggctga ggaccactaa cgagtactac
      901 atgctcaacg agcttgttac ggatgcccaa ctgcttgagc gcagtcctgc ctccggcaag
      961 ttgttcttca cgtactggat gtccagcacc aactacatca gcatgctcga ccccgatgga
     1021 ccgggactcg ctcagccgtt agcgagcgta aagggccttg gcaggataca gtggacggct
     1081 gcctctaccg aaggccccga cgtggtgctg tggggcatag gcaccaccgg cgggaggacc
     1141 gcggtcacgc ctgggcagca ggcgctggtc gtggtggcaa tacctccaag tggaccgccc
     1201 gtcgtggcgc agatggacat gggcgaattg tccaacatca ccatgatagc gcagcggggc
     1261 acggacctgg tctacctgct ctacggctac acgcgggaag ggcagctcaa gcgggccgag
     1321 ttgcgcagcg tgccgctatc gggggcgggg caaggccggc tcgaatctac gctgttgagc
     1381 acctatacct acccgtcgcg agggaagggc cgcttctact tcgatgaaga cttcaggttc
     1441 agctttggcc ccggcatgtt ggcatacctg gacggcagta ccctccgcgc cgtgacctac
     1501 gacgggcaaa ccgacctcgc cctggaaagc gacgtggcgg cgctgttcag ggtgggggtg
     1561 gccgaaccct gaccgttatc cttgttgctc aagtgggtaa tccgcataac ctgccgaacg
     1621 cgacttcgct ggtgtagcgt ccacgtggcg gtatatactg gtgccgatga gccgctattc
     1681 caatcgcctc ccggcccttg cctcgctcgc cctgcccgtg cttgcgctgc tggcgtgggc
     1741 cgggctggtg atgttgcttg cttaccaggc ccgccctgca tacgcggtga gcatggaccg
     1801 ctcgttcgac cgccattacc tcaccgacgg ctggcaccaa cccgaagaaa accccctgtt
     1861 caagttccgc tggatgtcgg ggtacgccac ggtcaggttc ccggcgattg gccgccaacc
     1921 cctcaagctc gtgatacaga tgggcacgca gggcatcgac ccgacgcctg agaagaaggt
     1981 gcaggtcacg gcacgggggg ctttggtcgc ggtgttcgac gtgcgcccct ctattgagag
     2041 gttcgaggtg ccggtctcgc ccgaactggt cagccgcgcc acgggcgacc tcgacctgct
     2101 cgtaaccatg ccaagcttcc agcctcccgg cgaccgaagg cagcttggcg tggtggtcac
     2161 cggcctgagt cttgagccgg acggcaccgg ggggcctgtg ctgccgcccg cttaccatgc
     2221 gctctacgta gcgggcatag gcgcgagcct ctatttcatg ctcctgttgc ttggaatagg
     2281 ggtgcgggcg aggttggccc tcagcatggt cgccgtgctg gcgatggggc tggtgtacgc
     2341 cttcgtccgg cccgctatcg ccttctacgc aggggacttg ctgctcgcgg cggtgctggc
     2401 gttggtggcc ctggcggtgg cgcagcctgc cgtgacgtgg ctgtacaggc gcgcaggcta
     2461 tagcgatttc aggcccagga gcggagaggg ccgcctgctg ttcgggctgt ttggctttgg
     2521 cctgctggtg ggctgggccg ggctgctgta cccccagagc gagccgcacg acttcctatt
     2581 ccacctgcac cgcttcaacg aggtgcaggc gggcaacctc ttcttcgaga actacgtggt
     2641 gtcgggggtg gggcagggct tctacccccc ggccatgtac gtgctgctcc tgcctttcag
     2701 cctgctcttg cgcgacccct acaatctcgt gctgctcgcc cccaccctgt tctccctcag
     2761 cgccgtcttt atcgtcttct acatcgcgaa gcggtacatg agcggctacc ggcacgcgcc
     2821 catactggct tcggcgcttt acgtggcgct gcccgtcaac ctgctagtca tctggtgggc
     2881 gcacgagacc aacttgttcg ggctggtcgt gctgctggcg gtggtggcct acgtgctagt
     2941 ggggtacggg cgcatcggcc gccccgccgt gtggttgggg ctggcggccc tgacgttcgt
     3001 gctgctgctg tcgcaccccg gcgtgctggt gtggagcgtg acgctgctgg ggggtaccgt
     3061 gctggcgttg ctgggcttgc ggcggttcgc ggccaacggc agctacaggt cggtgttgct
     3121 cgtagcgggg gcgttcgcgg cggcggggct gctggccttc gtcctctatt actcccgcta
     3181 cctcggctcg ttcgggcaag ccgccgtgga gcgtggcggg gaggtggatt tcgcggagac
     3241 gctgtcgagc gtgggcgacg tggaccggct tctggacatg atccggctca cactgtacca
     3301 cggcgtgctg ggcgactacg gcctgctacc cctcttgctg gtgccggtgg gcctgtggct
     3361 gctgtttcgc gggcgtgaca cggtgagtgc ggacgaggcg ataccacatg aggcatcaca
     3421 ggaggagcgg caacggttcc gctgggtgat gctggtgtgg gtcgcgtcgg ggctgctctt
     3481 cctgctcgtc agcctgctga ccgccctccc catccgcccg atgctcttca tgtggccccc
     3541 taccgcgcta cttgcagggc tgagcctggc ggccttcgtg gaaggcgaac gcgcgccggg
     3601 cagagcgtgg acctggcgca aagtcgctac actcggcctg ctagcagggc tggcggcgtt
     3661 gagcctctac ctgtgggcac tggcgaactt cctcgatatg cgggagccgc acgtgtaccc
     3721 gctggtgttc tagtgggagg gtggttgacg gaggttgtgg acacgctgcc acgttcgccc
     3781 tgcgagagcc ctgtgggtgt gccctgccac cgtagccctg ccaccccgcc ctgccaataa
     3841 attggcgggc ttaccagaga cgaagtctcc ttcggagact ctccctcatg cctcctagtc
     3901 cccgtagggg actttgcccc tggt
//
LOCUS       DASRWZ010000070         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1307374, whole genome shotgun sequence.
ACCESSION   DASRWZ010000070 DASRWZ010000000
VERSION     DASRWZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307374"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2110)
                     /locus_tag="VFH60_01000"
     CDS             complement(<1..2110)
                     /locus_tag="VFH60_01000"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6312385.1"
                     /translation="MVHFNPGFMDEVSNNLPVQPTPFIGRDKEVEAVCALLRREAVRL
                     VTLTGPGGTGKTRMGVQVGGQLLLDFRHGVYFVALASITDIALVATTIAQTLGIMEAA
                     HRPIIETIKAYLRDRHILLILDNFEQIVDASPVVADIMSGAAGLKVLVTSREILHLRG
                     EHEFPVPPLALPDTRHLPPPESLARYEAVRLFVDRAVALRPDFEVTSDNAPAVAEICA
                     SLDGLPLAIELAAARIKILSPEAMLSRLQNHGVAGGNGRLKLLVGGHRDLPARQQTLR
                     STIGWSYDLLNEGERMLFRRLSVFAGGRSFEAIEAVCNADGALPLDTLEGTASLVDKN
                     LLQQRPGPAGEDRFVMLETIQEYGREKLEESGEAEALQEQHAKYFMEMAERAEPELRG
                     PRQAEWLERLEAEHDNLRAAIRWATEQGHTEMALRLGAALVEFWKTRGHLTEGRTWLE
                     AALETSSTPTQARAKALMSAGNLASAQGDYARASAVLLESLELFRQLGDKVRIASTLK
                     NLGNDARLQGNYDAAYTYFEEGLQIARELGNRQETGTMLGDIGIVAQSMGDQEAARAY
                     YEESLQIRRELKDRRGIAMMLVNLGELARGEGDYDTAQGLYEEGLAIARELGDKWGVG
                     MVLHNLGHVAYHRQKYGQALDLFTQSMSIFYEMRNKRDIAYCLAALGGVYAAQRQPER
                     AAILFSAAQVLSNTISAHLDP"
BASE COUNT          379 a          800 c          734 g          432 t
ORIGIN      
        1 cggggtcgag gtgcgcgcta attgtgttgg agagcacctg ggccgccgag aataagatgg
       61 cggcgcgttc gggctggcgc tgcgccgcgt atactccccc cagagcggca aggcaatagg
      121 cgatgtcgcg cttgttgcgc atttcgtaga agatgctcat gctctgggtg aacaagtcga
      181 gcgcctggcc gtacttctgg cggtggtagg ccacgtgccc caggttgtgc agcaccatgc
      241 ccacgcccca cttgtcgcca agctcccgcg cgatagccag cccttcctcg tacagcccct
      301 gcgccgtgtc gtagtcgccc tcgccccgcg caagctcccc caggttcacc agcatcatcg
      361 cgatgccgcg cctgtctttc agctcacgcc gaatttgaag gctctcctca tagtaggcgc
      421 gggccgcttc ctggtcgccc atgctctgcg cgacaatgcc tatgtcgccc agcatcgtgc
      481 cggtctcctg gcggttgcca agctccctcg cgatttgcag cccctcctcg aagtaggtgt
      541 aagccgcgtc gtagttgccc tggagccgag cgtcgttgcc caggttcttc agcgtggagg
      601 caatacgaac cttgtcgcca agctgcctga acagttccag gctctccaac agcacggcgc
      661 tggcgcgcgc atagtctccc tgggccgacg ccaggttccc agccgacatg agggccttgg
      721 cgcgggcctg cgtgggggtg ctgctggtct ctagcgcggc ttccagccag gtgcggccct
      781 cggtcaaatg cccgcgcgtc ttccagaact ccacgagcgc tgcccccagc ctgagggcca
      841 tctcggtatg cccctgctcg gtcgcccacc gtatggccgc ccgcaggttg tcgtgctcgg
      901 cctccagccg ctccagccac tcggcctgcc tgggcccgcg cagctcaggc tcggcccgct
      961 cggccatctc cataaagtac tttgcgtgct gctcctgtag agcctccgcc tcgccgctct
     1021 cctccagctt ctcccggccg tactcctgga tcgtttcgag catcacgaag cggtcctcgc
     1081 ctgcggggcc gggccgctgc tgcaacaggt tcttatcgac cagtgaggcc gtgccctcaa
     1141 gcgtgtcgag cggcagagcg ccatccgcgt tgcacaccgc ctcgatagcc tcgaagctgc
     1201 gcccgcccgc gaacaccgac aaacgccgga agagcatgcg ctccccctcg ttgagcaggt
     1261 cgtagctcca gccgatcgtg ctgcgcaggg tctgttgcct cgcgggcagg tctcgatgcc
     1321 cacccaccag cagcttgagc cgcccgttac ccccagccac gccgtggttc tgcaagcgcg
     1381 atagcatcgc ctcgggggac aatatcttga tgcgggccgc cgccagctca atcgccaggg
     1441 gcaggccgtc caggctggcg cagatttcgg ccacggcggg ggcgttgtcg ctggtcacct
     1501 cgaaatcggg gcgcagggcc accgcgcggt ccacgaacag gcgcaccgct tcgtaccggg
     1561 ccaggctctc cgggggcggc aggtgccggg tgtcgggcag cgcgaggggc ggcacaggga
     1621 actcgtgctc tcccctcagg tgcaggattt cgcggctggt aaccagcacc ttcagccccg
     1681 ccgccccgct catgatgtcg gccaccacgg gggaggcgtc cactatctgc tcgaagttgt
     1741 ccaggatgag gaggatgtgc ctgtcgcgca ggtaggcctt gatcgtctct atgataggcc
     1801 ggtgggcggc ctccatgatg ccgagggtct gcgcgatggt ggtggcaacg agagcgatat
     1861 cggtgatgga ggcgagggcc acgaagtaga cgccgtgccg gaagtcgagg aggagttggc
     1921 cgcccacctg cacgcccatg cgggtcttgc ccgtgccgcc cggcccggtg agggtcacca
     1981 ggcgcaccgc ttcccgccgc aacagcgcgc acaccgcttc aacctctttg tcgcgcccga
     2041 tgaaaggggt gggctggacc ggcaggttgt tggacacttc atccatgaag cccgggttga
     2101 agtggaccat agcgcgcacc tcgctgtagg gccaccctgt aggcgcaaag ccctgcacta
     2161 cgacgggtcg gccttagatg attatacccg attgtggcgc ggttgactaa agtatcaaca
     2221 agtggctatg tggctctaca tagcctaagt cctccaagta cagaaaatta gatagttcga
     2281 tatcgtcaaa ttgcgcaaaa caagccggca ccggagaccc tactgcccta ccagaggtca
     2341 cctgt
//
LOCUS       DASRWZ010000071         3237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_210923, whole genome shotgun sequence.
ACCESSION   DASRWZ010000071 DASRWZ010000000
VERSION     DASRWZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210923"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1721)
                     /locus_tag="VFH60_01005"
     CDS             complement(246..1721)
                     /locus_tag="VFH60_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312386.1"
                     /translation="MNEGPKLKGILTRISLAATVLLGALPLGIMPVAAQSVPTESQFA
                     DRAFYTLWNRTDNLVASGKVARSYFWGPKPNTHGMVEQYVEGPGGTHLVQYFDKSRME
                     INDPDGDRNNPFFVTNGLLTVELITGRMQLGNRKHVSRWPARIPLASDTDDAEAPTYA
                     SFTSRLHGFTPDKTGEVVHEAIGKAADNGATDRFVKYNVKYFHFETATEHNVPDVFWN
                     FLSATGPVLVGGKVATAQLSNPWFYNTGYPITEAFWASVKIAGKPDTDVLIQPYERRV
                     LTYVPSAPEGYKVQMGNIGQHYYDWRYKDAGKPGPLPYPCTLPANFNDRFAKLWSSDV
                     VIQYQLHCPAAPPLGDENVTLAVQHFERGHMLYVGLKQNPPRTTTSARLIYVFLDDAT
                     IRTYPDRDPNTPEPTPEPAPPGLVTPVLGFGHVWRTHAEIKPLLGYATGPEKALKPGE
                     YSFFASGLMFADAGKIYTMFNPEFFYYAYANTWAVYDDPSQ"
     gene            complement(1801..>3237)
                     /locus_tag="VFH60_01010"
     CDS             complement(1801..>3237)
                     /locus_tag="VFH60_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312387.1"
                     /translation="VGALLVTLLSGLHAGGTVRQASAQASDYKFGDPAFRATWERTDR
                     LVDTGQVKRSFYWGPGPFEPARMEEYAEGPGGQHLVQYFDKSRMEINNPNGDRNNPFF
                     VTNGLLTRELATGSMQVGNSKVVERWPAEIPLASDADDATAPTYASFRTLITRPAPAK
                     AGLTLDERVSRDGVVSQDSSFARHGVRYAYYEAATKHNIPDVFWQFLNVTGPVIENGK
                     TVTALLNKPYFYATGYPIAEAYWATVKIEGKQGVAVLVQPYERRVLTYVPSAPEGFKV
                     QMGNIGRHYHDWRYKDAGRPVALQCNMAITDPRFSKVWSENASVQRQLGCMAGYAGFS
                     TLVVYQPFQNGVMLDVIITDQGSPRGGGSKQTKDIFVLYNDGTAQRFTDTYVDGAPLA
                     PVTAPAGLIVPQGGFGKLWRENSQVAQKIGYATAPEAGENAPNSSSLFLYFEKGRMVR
                     QGNQVFVLYAANGGSATDPNAWALFGNK"
BASE COUNT          527 a          926 c         1097 g          687 t
ORIGIN      
        1 ggctaacgtg gtgctcttaa cattgttaac gccatttctc ggaaaaaggt tgcagattat
       61 gtctatgcta agagacggaa ccgccaagac gtaaaggacg ccaagattaa gtaggtgttc
      121 taagcaaagg aaccagggta agataacatg taccctggtt ccttttgcac tctaaacctt
      181 tctactcttt gcgcgtcctt tgcgtccttt gcgtctttgc gttttcgtct ctttcccagt
      241 ctcggttact gcgaggggtc gtcgtacacc gcccaggtgt tggcgtaggc gtagtagaag
      301 aactcggggt tgaacatggt gtagatttta ccggcgtcgg cgaacatgag gccgctggcg
      361 aagaagctgt attctcccgg cttgagggcc ttttcggggc cggtggcgta gccgaggagg
      421 ggctttatct ccgcgtgggt acgccagacg tggccgaagc cgagtacggg cgttaccagg
      481 ccgggcgggg caggctcagg tgtaggctcc ggcgtgttgg ggtcgcggtc agggtaggtc
      541 ctgattgtgg cgtcatcgag gaagacgtat atgagccttg cagacgtggt ggtgcggggc
      601 gggttctgct tgaggccgac gtacagcatg tgcccgcgct cgaagtgctg cacagccagc
      661 gtcacgttct cgtcgccgag gggcggggcg gcggggcagt gtagctggta ctgtatcact
      721 acgtcgctgg accagagttt ggcgaagcgg tcgttgaagt tggcgggcag ggtgcacggg
      781 taggggagcg gccccggctt gcccgcgtcc ttgtagcgcc agtcgtagta gtgctggccg
      841 atgttgccca tctgcacctt gtagccttcg ggggcggaag ggacgtaggt gagcacccgg
      901 cgctcgtaag gctgtatcag cacgtcggta tcgggcttcc cggctatctt cacgctcgcc
      961 cagaacgcct ccgtaatggg gtagccggta ttgtagaacc aggggttgct gagttgcgcc
     1021 gtggcgacct tgccgccgac aagcaccggc ccggtggcgc tgaggaagtt ccaaaagacg
     1081 tcgggcacgt tgtgctccgt ggccgtctcg aagtggaagt acttcacgtt gtacttcacg
     1141 aacctgtcgg tagcgccgtt gtcggcggcc ttgccaatcg cctcgtggac cacctcgccg
     1201 gtcttgtcgg gcgtgaagcc gtgcaggcgg gaggtgaagc tggcgtaggt gggggcttcc
     1261 gcgtcgtcgg tatcgctggc gagggggatc ctggcgggcc agcggctcac gtgcttgcgg
     1321 ttgcctagct gcatgcgccc cgtgataagc tccacggtga gcaggccgtt ggtcacgaag
     1381 aaggggttgt tgcggtcgcc gtcggggtcg ttgatctcca tgcggctctt gtcgaagtac
     1441 tgcaccaggt gcgtgccgcc cggcccctcc acgtactgct ccaccatccc atgggtgttc
     1501 ggcttcggcc cccagaagta gctgcgcgcc accttgccac tcgccacgag gttatcggtg
     1561 cggttccaca gcgtgtagaa cgccctgtcc gcgaattgcg actctgtcgg gacggactga
     1621 gcggcgacgg gcataatacc gagaggtagc gcgcccagca ggacggtagc ggcaagggat
     1681 atgcgggtga gaatgccttt caacttgggt ccctcattca tataaagtgc tcttgtacga
     1741 ctcgtgtatc atctacggcc acacatgtga cgtatataga tgataggcgg ttgcacgcgc
     1801 ttacttgttc ccgaagagcg cccaggcatt ggggtcggtg gcgctcccgc cgttcgcggc
     1861 gtacagcacg aatacctggt tcccctggcg gaccatgcgc cccttctcga agtagaggaa
     1921 caggctgctc gaattgggcg cgttctcccc tgcctccggc gcggtggcgt agccgatctt
     1981 ctgggctacc tgcgagttct cccgccacag cttgccgaaa ccaccctgcg gcacgatcag
     2041 accggcgggg gcggtgacgg gcgcgagggg agcgccatcg acgtaggtgt ctgtgaagcg
     2101 ttgggccgta ccgtcgttgt acagcacgaa gatgtccttg gtctgcttgg agccgcctcc
     2161 gcgcgggctg ccctggtcgg tgatgattac gtccagcata acgccgttct ggaagggctg
     2221 gtacaccacg agggtgctga agcccgcgta acccgccatg cacccaagct ggcgctgcac
     2281 ggaggcgttt tcgctccaca ccttgctgaa gcgggggtcg gtaatcgcca tgttgcactg
     2341 gagggcaacg ggcctgcccg cgtccttgta gcgccagtcg tggtagtgcc gcccgatgtt
     2401 gcccatctgc accttgaagc cttcgggggc agaggggacg taggtgagca cccggcgctc
     2461 gtaaggctgg accagcacgg ccacgccctg cttgccctct atcttgacgg ttgcccagta
     2521 agcctcggca atggggtagc cggtggcgta gaagtagggt ttattcagca gggcggtgac
     2581 cgtcttgccg ttctctatta cgggtcccgt cacgttcaga aactgccaga acacgtcggg
     2641 gatgttgtgt ttggtggcag cctcgtagta ggcgtaccgc accccgtgcc tggcgaagct
     2701 ggagtcctgg gacacgacgc catccctgct aactctctca tccagggtca gccctgcttt
     2761 ggcgggggcg ggccgggtga ttagggtgcg gaagctggcg taggtgggag cggtggcatc
     2821 gtccgcgtcc gaggcgaggg gtatctcggc gggccacctc tcgacgactt tgctgttgcc
     2881 gacctgcatc gacccggtgg ctagctccct ggtgagcagg ccgttggtga cgaagaaggg
     2941 gttgttgcgg tcgccgttag ggttgttgat ctccatgcgg ctcttgtcga agtactgcac
     3001 gaggtgctgc ccgcccggcc cctcggcgta ctcttccatg cgggcgggtt caaatggccc
     3061 cggcccccag tagaagctgc gcttgacctg gcccgtatct acgaggcggt cggtgcgctc
     3121 ccaggtcgcc cggaaagcgg gatcgccaaa cttgtagtcg cttgcctgcg cggaggcttg
     3181 cctgaccgtg ccgcctgcat gcaagcccga cagcaacgtc accaataatg cgcctac
//
LOCUS       DASRWZ010000072         3937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_143020, whole genome shotgun sequence.
ACCESSION   DASRWZ010000072 DASRWZ010000000
VERSION     DASRWZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143020"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1608
                     /locus_tag="VFH60_01015"
     CDS             169..1608
                     /locus_tag="VFH60_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312388.1"
                     /translation="MATITANPGSSTAASPRSAATTQDRRVARLRSWMLAMISAALAF
                     GVLAGLVAWQANLATYNEYHTIVDEGAVSVDAALLARAAALDHMSAAATYLETEGAEQ
                     QQARAFADSRWDVFTEQARISWRNVTDPTHGELNVFSAADDASHDYIQQIGAMFGYEE
                     TGQRDRAGDAFLAARETMNTRLVPALGGLEAVKVEKMEATYAGADQRINNWRNVSLFV
                     GGLLALIFLGMLLAVRRMHYRWSWSIGAAFIATVLLTAGMQWHLLQASSDARVLVREA
                     YDNVAGVQDMAALLSQQRALDSIIVFDPAELDRRLADFDKYHTLVEQRLCGPRDCTQA
                     GTFLSNNDRISPLAVKAAQDEQDRLDLPRMPLVATVYFRGQADVYEQIRQDYRAWLVA
                     HETLVEHVRANRMAEAAAASSGESATTYAQLQQRAAEATQIARNEFNNIWQRVYTLTG
                     LGQVLALTFPLAGALAAWGLSRRRGELML"
     gene            complement(1631..1858)
                     /locus_tag="VFH60_01020"
     CDS             complement(1631..1858)
                     /locus_tag="VFH60_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312389.1"
                     /translation="MADPVFALTVFFEEPVPAEDAADWAHAIGMMRNVMKVEKHVQDA
                     AAVYAELKARRDLEQRLWDALHGKQKSPFRE"
     gene            complement(2209..3408)
                     /locus_tag="VFH60_01025"
     CDS             complement(2209..3408)
                     /locus_tag="VFH60_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6312390.1"
                     /translation="MHSQFPDGLFRLDDVLVAERARLVRLCARLSGSPDAAEDLVQET
                     LIEAWRIRDRLYDPSGYSQWLSAIARNVCRRWVRSHGRDLVHLAQPNGFDPLRSILEE
                     QPSNDFDLEVELEREELAVLLDRAMSLLPEATRAVLVQRYIEETPVAQVAERMGLSEG
                     AVEMRLQRGKLALKRVLATDLSEEASVYGLGGPDPSARQETRIWCPRCGQHRFLGRMS
                     HEAGTLTLWCPGCSPQLGTEIFSSGITHLLVGVKGYRAALTRLLTWVNHYYDEALNDF
                     AGLCAACGKQNTLHPTLPQQVTPRLGREPGLHILCSNCKRVSWASLRSLAQALPRVRE
                     FWKLHPRLHTLPMRELEANGRPAYMVSFRGVSDGAEMDVIIDRETFRVVGVHESQAAA
                     RVELQAV"
BASE COUNT          780 a         1306 c         1180 g          671 t
ORIGIN      
        1 agaggcacac tgttccccga attgttagac tcaaacgcga gcgactgatc cttcgctgcg
       61 ctcacgatga caggggtaac tggggcgccg cacaagaaga tcaatcatat tcacgaacca
      121 ggaaggaaag gtcgtagcac gtttcacatt tgagaagcag gaggaacatt ggcaacgatc
      181 acggcaaacc caggcagcag cacggcagca agccccagga gcgccgccac gacccaggac
      241 cgccgggtgg cgcggctacg gtcgtggatg cttgccatga taagcgcagc gctggcgttt
      301 ggggtgctgg ccgggctggt ggcctggcag gccaacctcg cgacctataa cgagtaccat
      361 accatcgtgg acgaaggggc ggtcagcgtg gacgcggccc tcctcgcgcg cgccgccgcc
      421 ctcgaccaca tgagcgcggc cgccacctac ctcgaaacgg aaggtgcgga acagcagcag
      481 gcccgcgcct tcgccgactc ccgctgggac gtctttaccg agcaggcccg catctcctgg
      541 cgcaacgtca ccgaccccac ccacggcgag ctaaacgtct tcagcgccgc cgacgacgcc
      601 tcgcacgact acatccagca gatcggcgcg atgttcggct acgaggagac aggccagcgc
      661 gaccgtgccg gagacgcttt cctcgcggcc cgcgaaacga tgaacacccg cctcgtcccc
      721 gcgcttgggg gcctggaagc cgtcaaggtc gagaagatgg aggccaccta cgccggggcg
      781 gaccagcgca tcaacaactg gcgcaacgtc tcgcttttcg tgggcggcct gctggcactc
      841 atcttcctgg gtatgctcct cgcggtgagg cgcatgcact acaggtggtc ctggtccatc
      901 ggcgcggcct tcatcgccac cgtgctgctc acggcgggga tgcagtggca ccttctccag
      961 gccagcagcg acgcccgcgt gctcgtgcgt gaggcttacg acaacgtggc cggcgtgcaa
     1021 gacatggcag ccctgctcag ccagcagcgc gccctcgaca gcatcatcgt gttcgacccc
     1081 gccgagctag accgccgcct ggccgacttc gacaagtacc acacgctggt cgagcaaaga
     1141 ttgtgcggcc cacgcgattg cacgcaggcg ggcaccttcc ttagcaacaa cgaccgtatc
     1201 tccccgctcg cggtaaaagc cgcccaggat gagcaggaca ggctggacct accgcgcatg
     1261 ccgctggtgg ccaccgtcta cttcaggggc caggccgacg tctacgagca gattagacag
     1321 gactaccgcg cgtggttggt ggcgcacgaa acgctggtgg agcacgtccg agccaatcgc
     1381 atggcggaag cggccgccgc ctcaagcggt gagagcgcca ccacctacgc ccaacttcaa
     1441 cagcgcgccg ccgaggccac gcaaatagcc cgcaacgagt tcaacaacat ctggcagcgc
     1501 gtctacactc tgaccgggct ggggcaggtg ctggcgctga cctttccact ggcgggggca
     1561 ctggccgcat ggggcctttc gcgcaggcgt ggcgaattga tgctgtgaag ccacctgcac
     1621 ggaataaagt tcattcccta aagggtgatt tttgtttccc gtgcagggcg tcccaaaggc
     1681 gctgctccag gtcgcgccgc gccttcagct ctgcgtagac ggcggctgcg tcttggacgt
     1741 gcttctccac cttcatgacg tttcgcatca tgccgatagc gtgcgcccag tctgcggcgt
     1801 cttccgcagg tactggctcc tcgaagaaga ccgtcagggc gaatacgggg tcggccactt
     1861 cactcctccc gacacactct gagtgctaca aggtcacata ctcaacgtag ggcctgtttg
     1921 caaagtgctt ttcgcgcggg accaggttcc cctttgtcat tctgagcgta gcgaagaatc
     1981 tcgtcctaca gcacccaaat accacctttc tccaagcctg caaggtgcta tgtgccggca
     2041 ggtgctctag aaaggggtaa cacctgcatg aaggacgaga ttcttcgctg cgctcagaat
     2101 gacaaaggcc acctgtggtc gcttgtttcc taccggttgc ggataatccc taatctttgg
     2161 acgtagccgc cggggccggc tcatgcaatc tatgggccac cctgagcatc agaccgcctg
     2221 tagctccacc cttgcggccg cctggctctc gtggacccct accaccctga acgtctcgcg
     2281 gtcgatgatg acgtccatct ccgccccgtc gctcacccct cggaagctga ccatgtaggc
     2341 cgggcggccg ttcgcctcaa gctcccgcat aggcagggtg tgcagcctgg ggtgcagctt
     2401 ccagaactcg cggacccgag gcagggcctg cgcgagggag cgcagagacg cccaggagac
     2461 gcgcttacag ttggagcaca atatgtgcag tcccggctcg cgccccagcc ttggggtcac
     2521 ctgctggggc agcgtcgggt gcagggtgtt ctgcttgccg caagcagcgc aaagccccgc
     2581 gaagtcgttt agtgcctcgt cgtagtagtg gttcacccag gtcagcagcc gggtgagcgc
     2641 cgccctgtaa cccttcacgc ccaccagcag gtgggtgata ccggagctga atatctccgt
     2701 gccaagctgg ggagaacagc cggggcacca gagggtgagc gtgcccgcct cgtgcgacat
     2761 gcgccccagg aagcggtgct ggccgcacct gggacaccat atccgcgtct cctggcgcgc
     2821 tgaggggtcc gggccaccca gcccgtacac ggaagcctcc tcgctcaggt cggttgccag
     2881 caccctcttg agcgccagct tgccccgctg aagccgcatc tccaccgcgc cctcgctcag
     2941 ccccatacgc tcggccacct gtgctaccgg cgtctcctcg atgtagcgct gcaccagcac
     3001 ggcccgagtg gcctcgggca gcagcgacat cgcccggtcc agcagcaccg caagctcttc
     3061 ccgttccagt tccacttcca ggtcgaaatc gttggacggc tgctcctcca ggatggagcg
     3121 cagggggtcg aacccattgg gctgggcgag gtgcacaagg tctcgcccgt ggctacgcac
     3181 ccagcggcgg cagacgttgc gggcaatcgc gctgagccac tgggagtagc cgctggggtc
     3241 gtacagccgg tccctgatgc gccacgcttc gatcagcgtc tcttgcacca ggtcctcggc
     3301 ggcgtcgggc gaaccggaaa gccgcgcgca cagccgcacc agccgcgcgc gctcggccac
     3361 caacacgtca tctagcctga aaagcccgtc cgggaattga gaatgcacaa aatccccctg
     3421 taaaggagtc gcaatactcc cgccattata acatcgctgc aaggccggac gaactaacgt
     3481 atttttcggg caatctgcga gaattgtccg cccgtaccgg cacatgtgcg tcttgtatca
     3541 ctttttgtcg cactccttgt actactcggt gtaacccaaa tctattctgt tgtccggccc
     3601 tcttgggtaa aatgagcaca gttggaacgc agcaagataa caaacaaaca cagacatata
     3661 aataccgaaa caacaatcca aaaaggcata tacaaaacgg acatagacag cgggcggcca
     3721 catatacagc ccggaacaat tttcaggagg gaacacgaca atgtacggta catatctctc
     3781 agagcagatg gcggcacagc gcacgcggga gaccctggca gtggtggagc gcaggcagca
     3841 ggaccggctg gtcgatcagg ccctcaagcg gtcacgccag ccccgcaagc cctccgggca
     3901 ccacattgtg cggctgggcc gcctggtagt aatgtgg
//
LOCUS       DASRWZ010000073         3981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_280238, whole genome shotgun sequence.
ACCESSION   DASRWZ010000073 DASRWZ010000000
VERSION     DASRWZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280238"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFH60_01030"
     CDS             complement(<1..287)
                     /locus_tag="VFH60_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312391.1"
                     /translation="MSQPQRSRCVWVLAASPVWSSPGRLRHLPSPDVVIAADGGTTLA
                     ARLGLAPDLVIGDMDSSDPALLQQLEAQGIPFDRSRHETKVETDTELAALAA"
     regulatory      complement(350..463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(553..1413)
                     /locus_tag="VFH60_01035"
     CDS             complement(553..1413)
                     /locus_tag="VFH60_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET6312392.1"
                     /translation="MPGIMLIDSPSNPIVKSLRSLAGGARQRRETGLFLAEGVRVVSD
                     ALDAGQVARSCLYDAEALARTEKGRLLVRRLQAVARSATGGVFEATARAVAAASDTQH
                     PQGVVAAFPVPHWTEPAGTGRAALALICDDIQDPGNLGTILRTAEASGVDAVWLTERC
                     VDLYSPKVVRAAMGAHFRLPCFSDATWPGIEASLENLGIQAANIYAAEAGATLNYDEV
                     IWNAPAALIVSNEARGLGEEARRLATRGGGLLAIPMLGGTESLNAAIATAVILFEAAR
                     QRRVATTAAK"
     gene            complement(1624..1962)
                     /gene="rplT"
                     /locus_tag="VFH60_01040"
                     /pseudo
     CDS             complement(1624..1962)
                     /gene="rplT"
                     /locus_tag="VFH60_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
     gene            complement(2070..2273)
                     /gene="rpmI"
                     /locus_tag="VFH60_01045"
     CDS             complement(2070..2273)
                     /gene="rpmI"
                     /locus_tag="VFH60_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET6312393.1"
                     /translation="MPKMKTHKGAARRFKITGSGKIMRTKHMKSHFRRRKSPRVKRMF
                     DRMFPLDPSQEGNVRKLLPYGVS"
     gene            complement(2431..3612)
                     /locus_tag="VFH60_01050"
     CDS             complement(2431..3612)
                     /locus_tag="VFH60_01050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6312394.1"
                     /translation="MPEYIMMMMPSQSSAELLAGYIRREIETHGPVTVARFIEWALYH
                     PEYGYYTRGPNIGPKGDFLTSPETSPAFGQLLASHVAEIDSLLEHPPKLHIVECGPGR
                     GTLAADLLEALASQFPDLLQRCHYWLVEISPALRAEQRQRLLPGFEGYVSWVAGMGEL
                     PSGVDGALIGNEFIDAFPVHVLENRDGAILEQYTGVGDEGAFELVYGQRSDAHLSEFL
                     SRYEIELAPGERIEINLAGAQWLNEAARVFSRGVVTLIDYGDTQPGRYSSSRREGTLL
                     GYYGGAVTHDILAHPGEQDITALVDFTALQDDAKRAGFSTVGMTRQATFLVGLGLGTS
                     VTVEGHTNDVTAALNYRRGLQTLVSMEGLGRFHVLLLGKGVDARVAAASLSGMKYAGL
                     L"
BASE COUNT          687 a         1292 c         1248 g          754 t
ORIGIN      
        1 gccgcgaggg ccgcaagctc cgtgtccgtt tcaaccttgg tctcgtgccg ggagcggtcg
       61 aacggtatgc cctgggcctc gagctgttgc aacaaggccg ggtcgctgct gtccatgtcg
      121 cctattacca ggtcgggggc caggcccagg cgggccgcca gcgtggttcc accgtctgcc
      181 gcgatcacca cgtcaggtga gggcaggtgg cgcaggcggc ccggtgagga ccacacgggg
      241 cttgcggcca gcacccatac gcagcggctc ctttgcggtt gggacatgcg cccttcctcc
      301 tctccggtgc aacaaaaaac cacatcccgg taggggggat gtggtcgttc aatcgataag
      361 tctgctttcc ctacgctggc attacccaga atcaggtcac agggtccgcg acgagccggt
      421 cgcgttctca gcctggcttg cccaagctcc cccagcaatc tatgcacttg ttaatcggcg
      481 tcttctggta ctatacacct tatgaggtaa aaatatcaac cacgcccgcg ccggaactga
      541 aaccagggcg ctctatttgg ccgctgtggt cgccacccgc ctctgccgcg ccgcctcaaa
      601 caggatcact gccgtggcta tagccgcgtt gagggactcc gtgccgccga gcatcggtat
      661 agccagcagc ccgcctcccc ttgtggcaag ccgccgggct tcctctccca ggccgcgcgc
      721 ttcattggag acgatcaggg cggcgggtgc gttccagatg acttcgtcgt agttgagcgt
      781 cgcgcccgct tcggcggcgt agatgttggc tgcctgaatg ccgaggtttt cgagtgaagc
      841 ctcgatccca ggccatgtgg cgtcggagaa gcagggcagg cgaaagtgcg cgcccatcgc
      901 ggccctgacc accttggggc tgtagaggtc cacgcaacgc tcggtaagcc agaccgcatc
      961 gacgcccgag gcctctgccg tgcgcaggat ggtgccgagg ttgccggggt cctgtatgtc
     1021 gtcgcagatg agcgcgagcg cagccctgcc ggtgccggcg ggttcggtcc agtgcggaac
     1081 gggaaaggcc gccactaccc cctgtgggtg ctgggtatcg ctcgcggcgg ccacggccct
     1141 ggcggtggct tcgaagaccc cgccggttgc cgagcgggcc actgcttgca gcctgcgcac
     1201 cagtagccgc cccttttccg tccttgcaag ggcttcggca tcgtagaggc aggacctcgc
     1261 tacctgccct gcatccagcg cgtcgctgac tacccgcacg ccttcagcaa ggaacagccc
     1321 ggtttctcgg cgttgccggg caccacccgc tagcgagcgt aacgacttta cgattgggtt
     1381 ggatgggcta tcgatcagca tgataccggg caaacaaaag ggcgaccgct catgagagag
     1441 tcgccctggg tgtcaacaaa ttaggtacgc cggtaatcag gcgtcggcgg tcgcggtagc
     1501 tgtctcgtcc gcgctcgtct caccggccgc gggcaatgca aggacctcgg ccatctcttc
     1561 ggcgtcggct atggcttgcg tggctacgcg ctggatcaat tcgggtgcga ccggcctgcc
     1621 gccggcgagc gctaccagtt gggcaaaact cgccgcgtcc cgcacggcca gctcggccag
     1681 catcttgcgg tcgatagtga ccccggcggc ggtaaggccg ctgatgaact tgctgtagga
     1741 cagcccgccc agccgggacg ccgcgttgat gcgggtgatc cacaggcgac gcatgtcgcg
     1801 cttgcttgtg cggcggtcgc ggtactgata ggcccgcgac ttcatgacga tctggttcgc
     1861 gaaccggaac aaacggctgc gcgtgcccct gaaacctgaa gcggccttga gtatcttcac
     1921 gtgccgccgg tgccttgtga cacccctctt tacgcgtggc atctctctat ttctcctttg
     1981 ttgaagctgc taccgtaggc taccggcctg ccaagccggt tattggaggc aatacggagc
     2041 cgcgagctga taatttgccg cgccgggcgt tacgagacgc cgtagggcag caacttcctg
     2101 acgttgcctt cctggctcgg gtcgagcggg aacatgcggt cgaacatccg cttcacgcgg
     2161 ggcgacttcc ggcggcggaa gtggctcttc atgtgcttgg tgcgcatgat cttgccggag
     2221 cctgtaatct tgaacctgcg ggccgcaccc ttgtgcgtct tcattttagg catggagtcg
     2281 cttgctcctg taagaataaa tggccgaata agcagcatct tttgcgacct gcatccgcgg
     2341 gccacagtcc gtaattatag catatgcacg cgtgcgatgc aaaccccctc gcagacgtaa
     2401 acgcgcctga aacccgcgcc ttcgccacca ctacagcagc ccggcgtact tcatgccgct
     2461 tagcgacgcg gcggccaccc gcgcgtctac acccttgccc aggagaagga cgtggaaccg
     2521 accgaggccc tccatcgaga cgagggtttg gagaccgcgc ctgtagttga gggcggcggt
     2581 aacgtcgttc gtgtggccct ccaccgtgac cgaagtgcca agccctagcc ccaccaggaa
     2641 ggtcgcttgc ctggtcatgc ccacggtgga gaagcccgcg cgctttgcat cgtcctggag
     2701 ggctgtgaaa tccaccaggg ccgtgatgtc ctgttcgcca gggtgcgcca ggatgtcgtg
     2761 agtgacggca cccccatagt aaccgagcag ggtgccctct cggcgcgaac tggagtagcg
     2821 gcccggttgg gtgtcgccgt agtctatcaa tgtgaccacc ccacggctaa agacccttgc
     2881 cgcctcgttg agccactggg cgccggcaag gttgatttcg atgcgctcgc cgggggcaag
     2941 ctctatctcg tagcgcgaaa gaaactcgct cagatgggcg tcggagcgct ggccgtacac
     3001 aagctcgaac gccccttcgt ccccgacgcc ggtgtactgc tccaagatag cgccgtcgcg
     3061 gttctcaagc acgtggaccg ggaaggcgtc tatgaactcg ttgccaataa gggcgccatc
     3121 caccccggac ggcagttctc ccatgcccgc aacccaggac acatatccct caaagcctgg
     3181 taataggcgc tgcctttgct cggcacgaag agcggggcta atttcaacga gccagtaatg
     3241 acagcgctgg aggaggtccg ggaattgcga ggcgagggct tccaggaggt cggcggccag
     3301 ggtgccacgg ccggggccac actcaacgat gtgaagcttc ggggggtgct ccagtaggga
     3361 gtcaatctca gcgacgtggc tcgcgaggag ctggccgaac gcgggcgagg tctccgggct
     3421 tgtcaggaag tctcccttag gcccgatgtt ggggccgcgc gtatagtagc cgtactcggg
     3481 gtggtatagc gcccactcga tgaaacgggc cactgttacg gggccgtgag tctctatctc
     3541 tcgccttata taaccggcga gtaattctgc acttgattgc gagggcatca tcatcatgat
     3601 atactctggc acaaggaatg ccaatcttct gagacgaagg actttagctt gactgctgcc
     3661 cagacttgtc gccggtgcca cactccgcta caggcgggtg ctgctgcgtg ccctatgtgc
     3721 gggctgcgtg tcgctccgcc cccaggaccc gccgccactc caccggcaca gcctcctgct
     3781 cgcccggagc acgtcgcacc gcctgcaccc caggagctgt ctgcttcaga caccgtgcgg
     3841 aacgaacccg ctgcgtccta tgctacaagt ccagctcctg gcccttctgt tgaccggccg
     3901 tctaccattc ccgtgtcccc acccaccgga cctacgcagc cacagcccgc gccgcccgac
     3961 cggggtggag atggcgcggg c
//
LOCUS       DASRWZ010000074         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1308107, whole genome shotgun sequence.
ACCESSION   DASRWZ010000074 DASRWZ010000000
VERSION     DASRWZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308107"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFH60_01055"
     CDS             complement(<1..285)
                     /locus_tag="VFH60_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET6312395.1"
                     /translation="MKLYFVYIMTNNNRTLYVGVTNNLERRVYEHKNKLIEGFTSRYN
                     LTELVYYAETNDIREAIAREKQIKGWVRARKVAMIEEENPLWVDLSADWFL"
     gene            complement(412..2049)
                     /locus_tag="VFH60_01060"
     CDS             complement(412..2049)
                     /locus_tag="VFH60_01060"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HET6312396.1"
                     /translation="MPGSIYVIGHKNPDSDSICSAVGYTALLHAQGQLNAVAARQGVL
                     RPETSYILDRFGIAAPLLVTDVRPRVADVMTTSVTTVGEDTSLFEVGQLLQRLGIRIV
                     PVVNGDGQLAGVIGVEDFARYFIAGLDLDELDRVPLELDNVLSALGGRVLVAAPGRTL
                     RDRVMVGAMRIDSMLKRLAPDIMMVMGDREDAQRAAIEFGVGALVITGDHPVSEEILA
                     LAREREVTIISVPHHTYTTVRLIHMSVPVRHVMNREPRTCGPNDLVEEVSERLRSGAA
                     RSLVVLRPDKTVMGFISRSSLLRSPRKQVILVDHNERAQSVEGIEEADVVGVIDHHRV
                     ADFQTRVPPFMRLEPVGATSTIVGKLYLESGLPIPQGMAGVLLGGILADTLVFRSATN
                     TPEDERVARILAERAGVELEEFGAAILSTASDVSDRTAEQIIASDFKEFTAGSSKFGI
                     GTIETTSGADVLSRRDEILEELGRLRSREGYDSAIFAVIDIVLGRTTLLVQGHTEAVA
                     SAFDTAAAGPDSVNLPGIVSRKKHIVPVLGTLARSIK"
BASE COUNT          400 a          787 c          685 g          447 t
ORIGIN      
        1 aaggaaccaa tcagcactca ggtctaccca aagcgggttc tcctcctcaa tcatcgcaac
       61 cttccttgcc cggacccaac ccttgatttg cttctcgcgt gcgatagcct cccgaatgtc
      121 attcgtttcg gcgtagtata caagctcagt aaggttgtac ctgcttgtga agccttcaat
      181 cagcttgttc ttgtgctcat aaacgcgccg ttcgaggttg ttcgtcaccc ctacatacaa
      241 ggtgcggttg ttgtttgtca tgatataaac gaagtacagt ttcatgtcac ggtcaaactc
      301 gggcggctga tccttcgcta cgctcaggat gacaaagggt ggctgggcac tcgcgctcca
      361 tcggtgatgc tattgccgga tgtgctatta ctgaccactg accactcgta attacttgat
      421 gctccgcgcc agcgtgccca ggaccggcac gatatgcttc ttgcgcgaca cgatgccggg
      481 caggttcacc gagtcggggc ctgcggcagc ggtgtcgaag gcggaagcga ccgcctccgt
      541 gtggccctgg accagcaggg tggtgcgccc cagcacgatg tcgattaccg cgaagatggc
      601 gctgtcgtac ccctcccggc tccgcaggcg gcccagctcc tccaatatct catcgcggcg
      661 agagagcacg tctgccccgc tggtcgtctc tatggtgccg atgccgaact tgctgctccc
      721 cgccgtgaac tccttgaagt cggaggcgat tatctgctcg gcggtgcggt ccgacacgtc
      781 cgaggcggtg ctcaggatgg cggcgccgaa ctcctctagc tccacgcccg cccgctcggc
      841 cagtatgcgg gccacccgct cgtcctccgg cgtgttggtg gcactgcgaa agacgagcgt
      901 atcggccagg atgcctccca gcagcacgcc cgccatcccc tgcggaatag gcaggcccga
      961 ctccaggtag agcttgccta caatcgtgct ggtcgcgccc accggctcaa gacgcatgaa
     1021 gggcggcacg cgcgtctgga agtcggccac cctgtggtgg tcgatcacac ccaccacgtc
     1081 ggcctcctct ataccctcca ccgactgcgc gcgctcgttg tggtccacca gtatgacctg
     1141 cttgcgcggc gaccgtagca ggctcgagcg gctgatgaac cccatcaccg tcttgtcggg
     1201 acgcagcacc accagcgaac gggccgcccc gctccgcagc cgctcgctca cctcttccac
     1261 caggtcgttg gggccgcagg tgcgcggctc cctgttcatc acgtggcgca cgggcacgct
     1321 catgtgtatc agccgcacgg tagtgtaggt gtggtgcggc accgatatta tcgtgacctc
     1381 ccgctcccgc gccagcgcca gtatctcctc ggacacgggg tggtcgcccg taatgaccag
     1441 cgcccctaca ccgaactcta tcgccgcgcg ctgggcatcc tcccggtcgc ccatgaccat
     1501 catgatgtcg ggggcgagcc gcttgagcat cgaatctatc cgcatcgcgc ccaccatcac
     1561 gcggtccctg agcgtgcggc cgggggctgc caccagcacg cgcccgccca gcgcgctcag
     1621 cacgttgtcc agctccagcg gcacccggtc tagctcgtcc aggtcgaggc ccgcgatgaa
     1681 gtaccgggcg aagtcctcca cgccgattac acccgccagt tggccgtcgc cgtttactac
     1741 cggcacgatg cgtatgccga gacgctgcaa tagctgcccc acctcgaaca gcgaagtgtc
     1801 ctcgcccacc gtcgtcacgg aggtggtcat cacgtcggcc acgcgggggc gcacgtctgt
     1861 gaccagcagg ggcgcggcga tcccaaagcg gtccagtatg taagaggtct caggacgcaa
     1921 cacaccctgc cgggcggcga ccgcatttag ctgcccctgc gcgtgcagca acgccgtata
     1981 accgacggcc gagcagatgg agtcggagtc ggggttctta tgtccgatta cgtaaatgga
     2041 gccgggcacg atgttccttc ctggtttggg gcctcttggt cgtacaaaat gcgtagcctc
     2101 ggcacgcggc acgcgccacc gggctaaatg cccgtacatt atgctggagc ctccccgttg
     2161 tgacaaggca cttagcccag gatgcggtcg ttcccggcac gagggcgcgt tcgtgccctg
     2221 cgatagccct gtgggggacg ccctgtggct aaagccacgg gcttaccaga gacgaagtct
     2281 ccttccgaga ctacctgatg ttgcttagtc cccgcaggg
//
LOCUS       DASRWZ010000075         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_143194, whole genome shotgun sequence.
ACCESSION   DASRWZ010000075 DASRWZ010000000
VERSION     DASRWZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143194"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..2694
                     /locus_tag="VFH60_01065"
     CDS             190..2694
                     /locus_tag="VFH60_01065"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET6312397.1"
                     /translation="MSRFSRAFTVSALVLLVAASVLSTTRYTSGARLSEAQAAPAYAP
                     DPASPFGIAGVMRWPDWGSFSQPADAFMQTGAAWVREDFAWGLIQPTEQRYEWTATDR
                     IVGGLTERKVNVLGIISYSANWATPSKEDDKQPSAISFYPPDPDKYAHFVRTLVSRYK
                     GTVKHWEVWNEPDNSLFWKPKPNAREYAELLKVAYKAIKQADPTAKVLSGGVSGNAIP
                     FLEEMVAAGAGSSFDILALHPYAVPLNPAQARVESRPEVHKMVDVELTKYRAFLERHG
                     PARPIWVTELGWPARDWGLDDAAQADYMAQAYAQMLASGLVERIFWYSFKDASDRPAD
                     SWGMLSWGKGKTDLSPRRPSFSAYATLTRMLAGAKPAGRVQLSRFSVVEDFEKPSTWA
                     RSFHAEGSMLAATGQARAGSGAGKLEYNFNAPNQAVDFAPDVPRPLPGAPTRLGLWVK
                     GDSSGNYLSAWLKDRDGELFKVRLGAVSAANDGWRYYESRTNNYYFDWERAGGSPSNG
                     RPDYPAHFVGFRLENTPDEPPGKGTVLVDDLQSYDGPDVSAVRFTRPDGQMVDVLWSV
                     DPTAVQLATASDKATVVDRDGKESQVTAKDGTLALKVSSSPIYVVHKPAEALGGTGAP
                     SPAGPGATQQPAKLERLAACVATDGVEASQEPDNRYFPETKHNLGGVFRQYWESHGGV
                     EVLGYPLTEVFTAPSSDGKSYRQQYFQRARLEHHPDNAPPNDVQVGLLGVWLTAGRTF
                     PASPAPTTGGEFFPQTGHGLGLFNEWWSAHGGLGVFGYPISGELQERNAADGKTYTVQ
                     YFERNRLEYHPENAGTNAEVLLGLLGVEYLKKQGCQ"
BASE COUNT          525 a          975 c          890 g          480 t
ORIGIN      
        1 ggaggcacaa tttagcgggc gtcttctctc ccaggcattc ctaataccaa cccccatcgc
       61 cacacaggac taaaggggtc gccactataa cccggttgtc ccttcctgcg ggttgcggca
      121 tcatgtataa tcctgtccat aactctcttt gccggctcgt ctcgcaccat tgatgaagga
      181 ccatcattga tgtcgcgctt ctcacgagca ttcacggtct cagccctcgt cttgctggtg
      241 gccgcgtctg tgttgtcaac aacccgctat acgtcaggtg cccgcctctc cgaggcgcag
      301 gcagcgcccg cgtacgcccc cgaccccgct tcccccttcg gcatcgccgg tgtcatgcgc
      361 tggcccgact ggggttcctt cagccagccc gcggacgcct ttatgcagac cggcgcggcc
      421 tgggtgcggg aggacttcgc ctggggcctc atccaaccca ccgagcagcg atacgagtgg
      481 accgccaccg accgcatcgt gggcggcctc accgagcgca aggtgaacgt gctcggcatc
      541 atctcctact cggccaactg ggccactccc tcgaaagaag acgacaagca gccctccgcc
      601 atatcgttct accctcccga cccggacaag tacgcccact tcgtgcgcac cctcgtctcc
      661 aggtacaagg gcactgtgaa acactgggag gtctggaacg agcctgacaa ctccctgttc
      721 tggaagccaa agcccaacgc ccgcgaatat gctgagctgc tgaaagtggc ttacaaagcc
      781 atcaagcagg ctgaccccac cgcgaaggtg ctctccggcg gcgtgtcggg caacgccatc
      841 cccttcctcg aagagatggt ggctgcgggc gcgggtagca gcttcgatat cctggccctg
      901 cacccctatg cggtgccctt gaacccggcg caggcccgcg tcgagagccg ccccgaagtc
      961 cacaagatgg tggacgtcga actgaccaag taccgcgcct tcctggagcg acacggcccc
     1021 gcgcgcccca tctgggtgac ggagttgggc tggcccgccc gcgattgggg cctcgatgac
     1081 gccgcgcagg ccgactacat ggcgcaggcc tacgcgcaga tgcttgccag cgggctggtt
     1141 gagcgcatct tctggtacag cttcaaggac gcgtcggacc gccccgccga tagctggggc
     1201 atgctgagtt ggggcaaggg caagaccgac ctctcaccac ggcggccctc cttctcggca
     1261 tatgctaccc tcacccgcat gctggcgggg gcgaagcccg cgggccgggt gcagttgtcg
     1321 cggttcagcg tggtggagga cttcgagaag ccttcgacct gggcgcgttc gttccacgcg
     1381 gaaggcagca tgctggcggc taccgggcag gcgcgggctg ggtcgggcgc gggcaagctg
     1441 gaatacaact tcaacgcacc caaccaggcc gttgacttcg cccccgatgt gccccgcccg
     1501 ctgcccggcg ctcccactcg cctggggctg tgggtgaagg gggacagttc gggcaactat
     1561 ctctcagcct ggctcaagga ccgagatggc gagcttttca aggtccgcct gggtgcggtc
     1621 tcggctgcca acgacggctg gcgctactac gagtcgcgca ccaacaacta ctacttcgac
     1681 tgggagcgcg ccgggggcag cccttccaac ggcaggcccg actacccggc gcactttgtg
     1741 ggcttccgcc tcgaaaacac gccggacgag cctcccggca agggcaccgt gctcgtggac
     1801 gacctgcaat cgtacgacgg gccggacgtg agcgcggtcc gcttcaccag gcccgatggg
     1861 cagatggtgg acgtgctctg gtcggtagac cccaccgccg tgcagcttgc caccgcctcg
     1921 gataaggcaa cagtcgtgga ccgcgacggc aaggagagcc aggtaacagc caaagatggc
     1981 actctggcgt tgaaggtgtc cagcagcccc atttacgtgg tgcacaagcc tgccgaagct
     2041 ctgggtggga ctggcgcacc gtcccccgct ggccccgggg cgacgcaaca gcccgccaaa
     2101 ctcgagcggc ttgctgcatg tgtagcgacg gacggagttg aggccagcca ggagcctgac
     2161 aaccgctact tccctgaaac caaacacaac ctgggcggcg tgttcaggca gtattgggag
     2221 agccacggcg gggtagaagt cctgggctac ccgctgacgg aggtcttcac cgcgccctca
     2281 tccgacggta agtcgtaccg ccagcagtac tttcaacgcg cccggctgga acaccacccc
     2341 gacaacgcgc cgcccaacga cgtgcaggtg gggctgctgg gcgtatggct cacggcgggg
     2401 cgcacctttc ccgcgtcgcc tgctcccacg accggcggtg agttcttccc acagaccggg
     2461 cacggcctcg gcttgttcaa tgaatggtgg agtgcgcatg gaggactggg tgtgttcggc
     2521 tacccgatct caggagagct acaggagcgc aacgcagccg acggcaagac ctacacggtg
     2581 cagtacttcg agcgcaaccg cctggaatac caccctgaga acgcgggtac caacgccgag
     2641 gtgcttctag ggctgcttgg cgtggaatac ctcaagaagc agggctgcca gtaaggtaac
     2701 cccacgccac gttcgccctg cggtgacccc ctgccactaa agtggcgggc tggcaagggg
     2761 caaagtcccc tacggggact aaggaacatc aggtagtctc cgaaggagac ttcgtccctg
     2821 gtaagcccgt ggctttagcc acagggcacc cccgccgggc acttctcctc
//
LOCUS       DASRWZ010000076         3681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_691542, whole genome shotgun sequence.
ACCESSION   DASRWZ010000076 DASRWZ010000000
VERSION     DASRWZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691542"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3195)
                     /locus_tag="VFH60_01070"
     CDS             complement(<1..3195)
                     /locus_tag="VFH60_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET6312398.1"
                     /translation="MSRFTYLYLHTHFSRKGGPASPSQWCHAATDLGYKSLGVADRGP
                     LAAFPAFSKAAREAGIQPIYGMEARISLPSSKDAKAGVTKAQPAVFFARDTEGLDNLA
                     SLASIAYARWPQEEQALDWDTLAQHASGLALVLLAESDALQDAAIASAGAKELAEWGA
                     SIKECFGDAVYVALAQPGNEGNQASTEKAAAAAQQMGLPTIAMSAARYIRSEDQLAYQ
                     ALQVARERAGWGGEVGAEAPSGGQGTQYLRSPDDAASLYAQWPEALENASRLAERCVG
                     VGYLDELATESFRASIQAEKTRLRRLAEERLLRKLGEDGLPDTVQQWLDDELSAYDRQ
                     NALPAWTALASVCDAALSHDGKPAVPLGAPVGTADGSLLAFAFGISPINPVDYPRPAW
                     LAAEAGTVGRVLPVPGVELPANRRDELLVALVRDYSPNRAALVACHIGIDPLTALSAA
                     SEVLGTGGDVRPLALQAMSQGWNALAADQAGGTPGRPTPASLALSLKGAPVTFKPDPD
                     MLLLAPVQGKLPGCLPVLRREGQPGAWVPVTEEAVAEMRLPAVMLRPSPTLSALASTL
                     TYAAQYPVPGLAVEELDLAAFPPPSTVAAGALARGELVGVPYLTVGAVKGWKGELTPA
                     DAAALVARSLSTGKPPTPPKLDQWTEQTAGTGGALLFRDQFEALVTATSGFSPIEAYS
                     LRVALLRADADGSTIEMKERFAAGCAANGLDAESIETLWQALAASAPDLQSRCSIAAR
                     ARASMWAAFFKAEHPAAYLAAHLAGSMDRGGVPLRALLGEAGRLKVGIKPPDASHSMP
                     APTLERGGSEWTILWGLALLPGWTRDVAHKFVAARPRGGFVKMADFALAAVDAGLTQE
                     QLESLVLAGGCDTLGGQARSREALIETLPAFLEWARAERGRDVAAGDAGPADLFSFND
                     AQPPAALPDEKPTLGGSTLTPRERYLRRAWEVAHIGAGFTPAGEIEALKRTLDSSHLH
                     SRLLRSVQVGEEHVGKSVNLVGLLTHIRLVSPPASNGNTNGNTNGNGHPPAAADTMSV
                     AWVEDTEGAIELVAFPPGYKRHAELWTENSLVIVT"
BASE COUNT          606 a         1212 c         1225 g          638 t
ORIGIN      
        1 ggtgacgatc accaggctgt tctcggtcca cagctccgcg tgccgcttgt agcccggcgg
       61 gaacgccacc agctcgattg cgccctcggt gtcctccacc caggccacgg acatcgtgtc
      121 ggcggctgcc gggggatggc cgttgccgtt ggtattacca ttggtattgc cgttggaggc
      181 gggcgggctg acgagcctga tatgggtaag cagacccacg aggttgacgc tcttgccgac
      241 gtgttcctcc cccacctgca cgctccgaag cagccgcgag tgcaggtgcg agctatccag
      301 cgtgcgcttc agcgcctcga tctccccggc gggcgtgaaa ccggctccga tatgcgccac
      361 ttcccaggcc cgccgcaggt aacgctcccg cggggtgagc gtagagccgc ccaatgtggg
      421 tttctcgtcg ggcagtgccg ccgggggttg tgcgtcgttg aacgagaaga ggtctgccgg
      481 gcctgcgtcc cctgccgcaa cgtcgcgtcc tcgctcggcc cgcgcccact ccaggaaggc
      541 gggtagggtc tctataagag cctcacggga gcgtgcctgc cccccgagtg tgtcgcaccc
      601 gcccgctagc accaggctct ccaactgctc ctgggtaagc cccgcatcga ccgccgcgag
      661 cgcaaaatcg gccatcttga cgaagccgcc cctgggccgg gccgccacga acttatgcgc
      721 cacgtccctc gtccagcccg gcagcagggc gaggccccag agtatggtcc attccgagcc
      781 gccccgctcc agcgtagggg cgggcatgct gtggctcgcg tcgggtggct tgatacccac
      841 tttcaaccgg ccagcttcac ccagcagcgc gcgcagcggg acgccgccac ggtccatgct
      901 cccagcgagg tgggcggcca ggtaagccgc ggggtgctca gccttgaaga acgccgccca
      961 catagacgcc ctggcgcggg ccgctatcga acagcgcgat tgcaggtcgg gcgcggaggc
     1021 ggccagagct tgccagaggg tttctatgct ctcagcgtcc agcccgttcg ctgcgcaacc
     1081 ggccgcgaac cgctccttca tttcgatggt ggacccgtcc gcatccgctc tcaagagggc
     1141 cacgcgcaga ctgtacgctt cgatggggga gaagccggag gtcgctgtga cgagcgcctc
     1201 aaattggtcg cggaagagga gggcaccccc cgtaccggcg gtctgctctg tccattggtc
     1261 cagcttggga ggcgtaggtg gcttaccggt ggagagcgag cgggccacca gcgcggcggc
     1321 gtctgccggt gtaagctcgc ctttccagcc cttcaccgcg cccacggtga ggtatggtac
     1381 gcccaccaac tcccccctgg caagcgcacc ggctgctacg gtgctcggtg gagggaaggc
     1441 cgcgaggtct agctcctcaa ccgctaggcc cggcaccggg tactgcgcgg cataggtcag
     1501 ggtgctcgcg agcgccgaca gggtaggcga aggccgcaac atgacagccg ggagccgcat
     1561 ctccgccaca gcttcctcgg tcacgggcac ccaggcaccc ggctgcccct cccggcgcaa
     1621 cacgggcagg caaccgggga gcttcccctg cacgggcgct aacagcagca tatcgggatc
     1681 gggcttgaat gtgacgggcg cgcccttcag cgaaagggcc aggctggcgg gtgtcggacg
     1741 cccaggggtg ccgccagcct ggtctgcggc gagggcgttc cacccctgcg acatcgcttg
     1801 caacgcgagg ggccgtacgt caccgccggt gcccagcact tccgaggcgg cagagagggc
     1861 cgtgaggggg tctatgccga tgtggcaggc caccagggcc gcgcggttgg ggctatagtc
     1921 gcggacgagc gccactaaaa gctcatcgcg ccggttggcg ggcagttcca cgccgggcac
     1981 cggcaagacg cgaccaaccg tgcctgcttc agcggcgagc caggccgggc gcgggtagtc
     2041 caccgggttg atgggcgaga tgccaaacgc gaaggccagc agcgacccgt ccgccgtgcc
     2101 gacgggcgca cccagcggga ccgcgggctt gccgtcgtgg ctgagggccg catcgcagac
     2161 cgaggccagc gccgtccagg cgggtagggc gttctgcctg tcgtacgcgg aaagttcgtc
     2221 gtccagccat tgctgcacgg tgtcgggcag gccgtcctcc ccaagcttac gcagcaaacg
     2281 ctcctcggcc agccgccgca accgggtctt ctcggcttgt atgctcgccc ggaacgactc
     2341 ggtcgcaagc tcatcgaggt agcccacccc cacgcacctc tccgcgagcc gggacgcgtt
     2401 ctccaacgcc tccggccatt gtgcgtagag gctagccgcg tcatcgggag agcgcaggta
     2461 ttgcgtgccc tgcccgccag agggggcctc cgcgccaact tcgccgcccc aaccggcacg
     2521 ctcccgcgct acttgcaggg cctggtacgc aagctgatct tcggagcgga tgtaacgggc
     2581 ggcggacatg gcaatcgtgg ggaggcccat ctgctgggcg gcagcggctg ccttttcggt
     2641 cgaagcctgg ttgccctcat ttccaggctg tgccagcgcc acgtaaacgg catctccaaa
     2701 gcactctttg atgctcgcgc cccattcggc cagttccttc gcaccggcgg aggcgatagc
     2761 cgcatcttgg agcgcgtcgc tttcagccag caggaccagc gccagccctg aggcgtgctg
     2821 cgccagcgtg tcccagtcga gggcttgttc ctcctggggc cagcgtgcgt aggcgatgga
     2881 ggcgagactt gccaggttgt ccagtccctc agtatcgcgg gcgaagaaga ccgcgggctg
     2941 ggctttggtc acacctgctt tcgcgtcttt ggaggagggc aacgatatgc gggcttccat
     3001 gccgtagatg ggctgaatcc cggcctcgcg ggctgccttg ctgaaggcgg ggaacgcggc
     3061 gagggggccg cggtcggcca cgcccaggct tttgtagccc aggtcggtgg cggcgtggca
     3121 ccactgggag ggcgacgcag gcccgccttt gcggctgaaa tgcgtgtgaa ggtacagata
     3181 agtgaatcgg ctcatgtaag gccgattata tacccggttc gcggctcgtg gattgggtgt
     3241 ggaaaggggt ggcggctgtg gaggatagag gagcttgggc gtttgcgtgt tctatggccc
     3301 tgctaaacgt cctggccttg aggtcgcgac caccctggcg gccgacttaa tcattcaggc
     3361 tagatgttat gtgctgtaag acacgggtaa gttatacgct tagacagtac acccgttagt
     3421 gttggtcttg cgaggaagca tcctttagct cgttggcccc aatcttccca agaacttctt
     3481 gcacaagttc atcaaaaacc tgcttaccca tctgatcgcg aatgcgagct aaagctctac
     3541 ctactacatc gcgatagcgc tgaggcatcc tgagatgctc cattagtacg aaggcttgaa
     3601 ctaacatcgt cactgcatct cccaagttgc tttctcgctc cagtagcagc cctaactggc
     3661 ccagcgagga agctattcaa g
//
LOCUS       DASRWZ010000077         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1034132, whole genome shotgun sequence.
ACCESSION   DASRWZ010000077 DASRWZ010000000
VERSION     DASRWZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034132"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="VFH60_01075"
     CDS             complement(<1..1567)
                     /locus_tag="VFH60_01075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET6312399.1"
                     /translation="MNRQRIESAYIDPQPTTEFVRMIPIGGLGEVGKNMAVVEYGEDI
                     VIIDVGMGFPEEEMFGVDLVLPDISYLNGKEHRIRGICITHGHEDHIGGLAYLLPQLK
                     APVYSRPLTLGLIRNKLRERGLLEETDLREVNAGDVVQLGEMRVEFVHTCHSIPDATM
                     LAVHTPIGTIVHTGDFKLDPTPVDGYPPDFASLQRLGDDGVLGLFSDCVHVETPGYTP
                     SEQMVGRTLGPIIANAPGRVIIATFASLISRVQQIMDLAYKHNRKVALLGRSLENNVQ
                     MAVELGYLQIPENLIVPVGDTGKFYDNEVLVICTGAQGEPTSALSRIANDDSRHWSVK
                     PGDTYILSASPIPGNETSVGRVINNLFLKGAEVIYSHIAQVHVSGHASQEEHKLVLNL
                     LRPQYVIPIHGERRHLVLYTKIAQSMGVPPENTFLVDNGYVVELNRDEGRIAGRVPAG
                     AVYIDGLSVGGIGPVVIRDRQMLARDGVLLVVVTIDKQTGQLVTTPDIVTRGFVYAAE
                     AGPLLEETKERVRN"
     gene            complement(1560..>2247)
                     /locus_tag="VFH60_01080"
     CDS             complement(1560..>2247)
                     /locus_tag="VFH60_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312400.1"
                     /translation="NTRMSDVDAQASAAPAQVLTSSVSNEYHMSRDGKRQGILLLLGV
                     LSLEVFAVWSLATILDRGLEGAEWVSALLMLGIAMVAPLVAWTLLEELNSRYVVAAEG
                     LRYNSIGGIDLLYPWEQLAGFKTEQRRGRVARFFLGEEDGTDNETNAGVTTPGGTNEV
                     EEESEAEPQALALHVRESYRSQGESQLGNPLLRFLHTQAHGNTIPLHGGIENRDELVS
                     QIASRIRNNE"
BASE COUNT          381 a          745 c          670 g          451 t
ORIGIN      
        1 cgttacgcac ccgctccttg gtctcctcca gcaggggacc ggcttcggcg gcgtacacga
       61 aaccgcgcgt cacgatgtcg ggcgtggtca ccaactgccc ggtctgcttg tctatcgtca
      121 ctaccacgag cagcacgcca tcacgggcga gcatttgccg gtcgcgaatc acaacgggac
      181 cgatgccgcc gacgctcaac ccatcgatgt acaccgcgcc cgcgggcaca cgccctgcga
      241 tgcggccctc gtcccggttc aactcgacca cgtagccgtt gtccaccagg aacgtgttct
      301 ccggcggcac tcccatcgac tgcgctatct tggtatagag caccaggtgt cgccgctcgc
      361 cgtgaatcgg tatgacgtat tgcggacgga gcaggttcag cacgagcttg tgctcctcct
      421 ggctggcgtg gcccgacacg tgtacctggg cgatgtggct gtaaatgacc tccgcgccct
      481 tgaggaacag gttgttgata acccgcccca cgctcgtctc gttaccgggg atgggcgagg
      541 ccgataggat gtaggtgtcg ccgggcttga cgctccaatg ccggctgtcg tcgttggcga
      601 tgcgcgagag ggccgaagtc ggctcgccct gcgcccccgt gcagatcacc agcacctcgt
      661 tgtcgtaaaa cttgccggtg tcacccacag gcactatcag attctcgggt atctgcaagt
      721 agccaagctc caccgccatc tgcacgttgt tctccaggct gcggccgagc agcgccacct
      781 tgcgattgtg cttgtaagca aggtccatga tttgctgcac ccgcgagatg agcgaggcga
      841 aggtggcgat aatcacccgg cccggcgcgt tcgcgatgat agggccgagc gtgcgcccga
      901 ccatctgctc cgagggagtg tagcccggcg tctccacgtg cacgcagtcg ctgaacagcc
      961 ccagcacgcc gtcgtcgccc aggcgctgca agctggcgaa gtcgggcggg tagccgtcca
     1021 cgggggtcgg gtcgagcttg aagtcgcccg tatgcactat cgtgccgatg ggggtatgca
     1081 ccgcaagcat ggtggcatcg ggtatagagt ggcaggtatg tacgaactcc acccgcatct
     1141 caccaagctg caccacatcg cctgcgttca cctcgcgcag atccgtctcc tccagcaggc
     1201 cgcgctcgcg cagcttgttg cgtatcaggc ccagcgttag cggcctcgag tacacagggg
     1261 cctttagttg gggcagcagg tacgccaggc cgccgatatg gtcctcgtgc ccgtgggtga
     1321 tgcagatgcc ccggatgcgg tgctccttgc cgttgaggta gctgatgtcg gggagcacga
     1381 ggtccacccc gaacatctct tcttcgggga agcccatgcc cacgtctatg attacgatgt
     1441 cctcgccata ttcgaccacg gccatgttct taccgacctc gcccagtccg ccaatgggta
     1501 tcatgcgcac gaactcggtc gtgggctggg ggtcgatata cgcgctttct attctttgcc
     1561 tattcattat tccttatgcg cgaggcaatc tgcgacacaa gctcgtccct gttttcgatt
     1621 ccaccgtgga ggggtatcgt attcccgtgc gcctgggtat gcaggaaccg cagcagcggg
     1681 ttgccaagct ggctctcccc ttgcgacctg taactttcac gcacgtgcag ggcgagggct
     1741 tgcggctctg cctcgctctc ctcttctact tcgtttgtac cgcccggagt ggtgaccccg
     1801 gcgtttgttt cgttgtcagt cccgtcttct tcgcccagga aaaagcgtgc tacgcgcccg
     1861 cgccgctgct ccgtcttgaa cccggccagt tgctcccacg ggtagagcag gtcgatgcca
     1921 cctatgctgt tgtagcgcag gccctcggca gcgactacgt accggctgtt cagttcttca
     1981 agcaacgtcc aggccaccag gggcgcgacc atggcgattc ccagcatcag cagcgccgac
     2041 acccactcgg ccccctccaa cccacggtcc aggatagtcg caaggctcca gacggcgaac
     2101 acctcaaggc tcagcacgcc aagcagcagg agaatcccct gccgcttgcc gtcccgcgac
     2161 atgtggtact cgttcgacac cgaggacgta agtacctggg cgggcgcggc gctggcctgc
     2221 gcgtcaacat ccgacattcg cgtgttg
//
LOCUS       DASRWZ010000078         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_965537, whole genome shotgun sequence.
ACCESSION   DASRWZ010000078 DASRWZ010000000
VERSION     DASRWZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965537"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFH60_01085"
     CDS             complement(<1..436)
                     /locus_tag="VFH60_01085"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HET6312401.1"
                     /translation="MSAISKSGQVNTQGCEDDIPQPIVNLNRWTLVLGIVGGLVLQQP
                     LFTTVLFLILVPAVLYGQRGSLIYQLGKRVLPGTEKGTPGVDRRLMRFNNSIAVVLLG
                     SAQVAFLFGSAAVGWALALMVAIAASVARAVYCVGCTIYYRFR"
     gene            complement(465..908)
                     /locus_tag="VFH60_01090"
     CDS             complement(465..908)
                     /locus_tag="VFH60_01090"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET6312402.1"
                     /translation="MTLYLDDCARDSIVQHGEGGYPNEVCGILLGKDIDGRRVIRLTM
                     PIENSFEQDEQYHRFLITPADMFRAERLARHERLDVLGVYHSHPNAPAQPSEYDRDHA
                     AWTTWSYVIVSVQEGKAADILAWKLREDRSAYDEEAVEVSATSRT"
     gene            complement(927..2204)
                     /locus_tag="VFH60_01095"
     CDS             complement(927..2204)
                     /locus_tag="VFH60_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HET6312403.1"
                     /translation="MSSGEGQGKSESKVGRGPGTRAVHAGSHADLRGTRPSNVPIYAS
                     STFLSPDAATLDGVLGGTEPGYVYGRYANPTVAALEEALASLDGAAASVAFGSGMAAL
                     HAAFLLCELEPGNTVLAGSDLYGASHTLLATLFGQFDVQTRFADMTDFEAVAQALRTE
                     PRPRAMLFETASNPLIKVADVPRLCELARALGVLTIVDSTFTPPPIIQPLALGADMVM
                     HSDTKYFGGHGDVTGGHVSIADPALEPTLRQISRLAGAIPGPFDAFLTSRGVKTLVVR
                     VRQQCENAAILARRLAEHPLVARVHYPGLPGHPQHDVAARIFCPPYMGAMLALEIAGA
                     GKDEMLRFMDALELVIPATTLGDVYTEVSYPVISSHREWAPAQLRRAGITPGLVRVSA
                     GIEDVEDVIEDIDQALHAAVGAVTTQATDTANQ"
     gene            complement(2201..>2948)
                     /locus_tag="VFH60_01100"
     CDS             complement(2201..>2948)
                     /locus_tag="VFH60_01100"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET6312404.1"
                     /translation="GLLSPGTTILDASSGNTGIAYAMIGAALGYKVEICLPESASPER
                     KRLLKLYGATIIDTPPADGTDGAILEARRLYAERPGRYFYPDQYNNPANWQAHYYGTA
                     PEIWQQTDGRVSHFVAVVGTSGTFVGASRRLKQYNPGIRIVEVEPDAAFHGLEGMKHM
                     PSALVPGIYDPNLADEQVLIGTEEAQAMTRELARYEGVLAGPSGGAGVLAAVRVARSL
                     RSGVVVTVLPDGGSRYLQDTFWDESEGGVR"
BASE COUNT          478 a          970 c          977 g          523 t
ORIGIN      
        1 tccggaagcg gtagtaaatg gtgcagccga cgcagtaaac ggcacgagct acactggcgg
       61 caatcgccac cattagggcc agcgcccaac ctaccgcagc cgagccgaaa aggaacgcca
      121 cctgggcaga gcctagcaga actaccgcta tcgagttgtt gaaccgcatc aggcggcggt
      181 ctacccccgg agtgcctttc tccgtgccgg gcagaacgcg cttgccaagt tggtaaatga
      241 ggctgccacg ctgcccgtac agcaccgccg ggaccaatat gaggaacagc acggtggtga
      301 acaggggctg ctgcaacacc aagccgccca cgatgccgag aaccagggtc caacggttga
      361 gatttacgat gggctgcggt atgtcgtcct cgcaaccttg tgtgttcact tgcccgctct
      421 tgctaatcgc gctcataact cttcctccaa ttaattctct ctttttacgt cctgctcgtc
      481 gcgcttactt ctactgcttc ttcgtcatat gcgctgcggt cctcgcgcag cttccaggcc
      541 agtatgtcgg cagccttgcc ctcttgaacc gaaacgataa cgtagctcca agtggtccag
      601 gcggcgtggt cgcggtcgta ctcggagggt tgggcggggg cgttggggtg cgagtggtac
      661 acgcccagca cgtcaagacg ttcgtggcgc gccagccgct cggcccggaa catgtcggcg
      721 ggcgtaatca ggaagcggtg gtactgctcg tcctgctcga acgagttctc aatcggcatc
      781 gtcagccgta tcacgcggcg gccatcgatg tccttgccga gcaggatgcc gcacacctcg
      841 ttggggtagc ctccctcgcc gtgttgcact atcgaatcgc gtgcgcaatc gtccaggtac
      901 aatgtcattt ctcggtccct ttgcctctac tggtttgccg tatcagtagc ttgtgtggtt
      961 acagcgccca cagccgcgtg gagcgcctgg tctatatctt cgatcacgtc ctctacgtcc
     1021 tcgatgcccg cggagacccg caccaggccc ggcgtgatgc ccgcccgccg cagctgagcg
     1081 ggtgcccact ccctgtgact tgagatgacc gggtaggaca cctcggtgta cacgtcgccc
     1141 agggtggtgg cggggatcac cagctccagc gcgtccatga accgcagcat ctcgtccttg
     1201 cccgcgcccg ctatctccag cgccagcatg gcccccatgt agggcgggca gaagatgcgg
     1261 gcggccacgt catgctgcgg gtggccgggc aggccagggt aatgcaccct tgccaccagc
     1321 gggtgctcgg ccaaccgccg cgccaggata gccgcgttct cgcactgctg cctcacccgc
     1381 actaccagcg tctttacccc ccggctggtt agaaacgcat cgaaaggacc ggggatggca
     1441 cccgccagcc tcgatatttg cctcaacgtc ggctccaatg cagggtctgc tatcgacacg
     1501 tggccgcccg ttacgtcgcc gtgcccgccg aagtacttgg tgtcgctgtg cattaccatg
     1561 tccgcgccga gggccagggg ctggatgatg ggcggcgggg tgaaggtcga gtccacaatg
     1621 gtgagcacac cgagggcgcg ggcaagctcg cacagccggg gcacgtcggc caccttgatg
     1681 agcgggttgg aagccgtctc gaacagcatg gcgcggggcc gcggctcggt gcgaagggcc
     1741 tgcgccaccg cctcgaaatc ggtcatgtca gcgaagcggg tctgcacgtc gaactggccg
     1801 aacagggtcg caaggagcgt atggctcgcg ccgtacaggt cgctgcccgc cagcaccgtg
     1861 ttgcccggct caagctcaca cagcaggaag gccgcgtgca gggccgccat gccgctgccg
     1921 aacgccacgg aagccgccgc accgtccagg gaagccagcg cctcctcaag ggccgccacg
     1981 gtagggttgg cgtagcgccc gtacacgtag ccgggttcgg tgcctcccag caccccatcc
     2041 agggtggcgg cgtcggggct aaggaaggtg ctgctggcgt agatgggcac gttcgaggga
     2101 cgggtgcccc gcaggtcggc gtgcgagccg gcatgcacgg ccctggtgcc cggaccccgc
     2161 ccgaccttgc tttcgctctt cccctggcct tcgcccgaac tcacctgacg ccgccctcgc
     2221 tttcgtccca gaaggtatct tgcaggtagc ggctgccgcc gtcgggcagc accgtcacca
     2281 ccacgccgga gcgcagggag cgtgccacgc gcacggcggc cagcaccccc gccccgcccg
     2341 aaggccccgc cagcacgccc tcgtatcgcg caagctctcg ggtcatggcc tgggcctcct
     2401 cggtgccgat aagtacctgc tcgtcggcaa ggttggggtc gtagatgccg ggcaccaggg
     2461 cggagggcat gtgcttcatc ccttcgaggc cgtggaaagc cgcgtcgggc tcgacctcca
     2521 cgatgcggat gccggggttg tactgcttga ggcggcggga cgcgcccacg aaggtgccgc
     2581 tggtgcccac caccgccacg aagtggctca cgcgcccgtc ggtctgctgc cagatttcgg
     2641 gggcggtgcc gtagtagtgg gcctgccagt tggcggggtt gttgtactgg tcggggtaga
     2701 agtagcggcc ggggcgctcg gcgtagaggc ggcgggcttc gaggatggcc ccgtcggtgc
     2761 cgtccgccgg gggcgtgtcg atgatggtcg caccgtacag cttcagcagc cgcttgcgtt
     2821 cggggctggc cgactcggga aggcaaatct ccaccttgta gccgagggcc gcacctatca
     2881 tggcgtaggc gatgccggtg ttgccggagc ttgcgtcgag gatggtcgtg ccgggtgaga
     2941 ggaggccg
//
LOCUS       DASRWZ010000079         7145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_760293, whole genome shotgun sequence.
ACCESSION   DASRWZ010000079 DASRWZ010000000
VERSION     DASRWZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760293"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFH60_01105"
     CDS             complement(<1..256)
                     /locus_tag="VFH60_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312405.1"
                     /translation="MPASPIRGGYKLLSLVAALALLATQANPTPTNAATQAQPACGAW
                     SIVPTSGTALDLGRFTGVAATSYSNVWAIGSTSDQNDLLAR"
     gene            complement(369..1265)
                     /locus_tag="VFH60_01110"
     CDS             complement(369..1265)
                     /locus_tag="VFH60_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6312406.1"
                     /translation="MSRDERQGLLYVALAVFFFSTSPVFIVWADPIDPFIKTWGRMLV
                     AALAVGGLAWLTRDRARDEGMAAGVAARSRGNSTLRFLTYGLVAALHFLFYISSLSFT
                     TAAHALSLIYTAPIFVALFSALFLKEQLRSRQWAGIGVTVLGVAILAGLEPHMDLEMA
                     FGDLLALISAVMFGLYSVAGRYERERYPLLVYASRVYWSATLWLLPLALFTIPRMSPE
                     AWDWTKLGAIAAVGLLSGALGHTLYNASLRRTHAAYVNIIASQEVTGGIILSWIFLSQ
                     VPTPNSIAGALVTLVGIGLVLR"
     gene            complement(1262..2323)
                     /locus_tag="VFH60_01115"
     CDS             complement(1262..2323)
                     /locus_tag="VFH60_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6312407.1"
                     /translation="MSASYEDSLWGDILIDRPVLAELVESAPVQRLKGIHQAGASVYL
                     LPGTRHATRYEHSLGVLHVLSRLGAGLEERVAGLLHDVPHTAFSHTADIVFPNDEHNF
                     HERFQHDVITRSTVPAILRRHGVSPHAALEPDSYPLLERPLPGLCADRIDYTLRDLRA
                     RHQVDPHHAADFLSHLLPTPSGILVDHTEAAEWFAHLFSEANNRYWTGPQEAGAYWAL
                     AGAIRRGYETGDFTDKDLFSTDAEAMSRLRSLDDPEVQAYLTLLTPGTRFYEVDDGGP
                     CFSTHMKQRYVDPTVLEQGWPAPRKLSEVSQTYRRMLGLFPTARNVYYKLWTDAMPGI
                     IADKLRAQEGKTPAHGPSL"
     gene            complement(2417..3142)
                     /locus_tag="VFH60_01120"
     CDS             complement(2417..3142)
                     /locus_tag="VFH60_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312408.1"
                     /translation="MALMNPVRELPLLSAATPFNKIVVQARTLLPTLLLVYAPALLVL
                     VAVFAASLTSNVPVAYFLRDPAGILHVPNYVGVVSGLGVLLWCSCAAVCLFSWTVLKK
                     AGATSRWPAFFFAAGIVTTVLLIDDFFMLHENASQIYWRFGEETVFALYGFMFVLFIA
                     RFWRTIASTEFLVFLLACGFMGMSLVFDKLNGPYGDNVPAIRLLLEDGAKLLGIVAWL
                     VYFARTCLAQTIPLFARERPATH"
     gene            3831..5258
                     /locus_tag="VFH60_01125"
     CDS             3831..5258
                     /locus_tag="VFH60_01125"
                     /inference="COORDINATES: protein motif:HMM:NF041539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor R domain-containing protein"
                     /protein_id="HET6312409.1"
                     /translation="MHGLMNSIWNSRFSRTFLAVIPLLVIPLVISLGVAPNARDTSAF
                     AASPTVLHDQLNNFEGTIVSFENGTNFSSQAADDFIVPPGKTWTLTQVDVSGFPVVGS
                     DQPAFFNVFLYRDNGTLPGVQVASYSNLSYAFNSTNGEYSIAFPSTLLKAGTYWVSVQ
                     ADTTAAGNRWTWTGRLTPAVNNGAAWRNPGNGFSSGCTDWGTKTACQSATFSGPDQTF
                     RLHGTEETPVPAPVLHDQLNKFEGSIVSFENGTNFSSQAADDFMVPPGKTWTLTQVDV
                     SGFPVVGAAQPTSFNVFLYSDSSTLPGSQVASYSNLDYAFNTTNGEYSIDVPPTSLGS
                     GTYWVSVQADTTAAGNRWTWTGRLTPAVNNGAAWRNPGNGFGSGCTDWGRKTTCQPAT
                     LSGPDQTYRLHGIEATPTATPTSPPAATATPVRGVPPGFEPHYACRATKDGALRDTGV
                     LVAVGPRPTCLKNEVLVAILVNDAP"
     gene            complement(5403..6689)
                     /locus_tag="VFH60_01130"
     CDS             complement(5403..6689)
                     /locus_tag="VFH60_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6312410.1"
                     /translation="MMTLNAAIPAHRKPIVVLLVSVFVLALFGFSRPIAAQPSAPSAP
                     QATCPAGGQCFSDVLPGSPFYDYANNLYMQDIISGYPCGGPGEPCDGQNRPYYRSGNS
                     VTRAQMTKFVDLARKQPGIYIDTPSHIQPLYSRTTAAAGVGVTGASGNGTGVLGYSDM
                     GTGVQGLTSSPITDAVGIMGMVANTSAGRDSAGVRGRNQGTGGYGIGVWGSHDGAGPG
                     VKGTGTNGFGGEFTSENYRGAYVRGGPSWIALYADGWTYVNGNLDVTSHAYVSGDLTV
                     DGDCTGCTQAYVARNSGDESIRLGDLVAAAGVEVDPKSGQPVLLVRRAGLPGDAVIGV
                     AGTSLVRDTAGERAKEQNQPLVPAEGAAAPNTYVRVIVSGLAQVRVTTWAVAIGDSLA
                     PDSSGGATQASQGAVARAVSTPDAQGLVWALVSAGR"
BASE COUNT         1362 a         2074 c         2362 g         1347 t
ORIGIN      
        1 aacgcgctag caggtcattt tggtcggagg tgctgccgat ggcccacacg ttggagtagg
       61 aggtggctgc cacgcccgtg aagcgtccca ggtcgagggc tgttcctgag gtggggacga
      121 tggaccacgc gccgcaggcg ggttgggctt gcgtggcggc gtttgtaggt gtggggttgg
      181 cctgggtagc gagcagggcg agggcggcaa ctagcgacag cagcttatat ccgccgcgaa
      241 tgggtgatgc gggcattggt ctaccttacg tatcaaactg gggcatgaca ttgtagcaaa
      301 ggggtggatg ttgtgtcaaa gtcggggaag tgagtaccct cccaagtttg gcgcgtcgca
      361 agggcgtttc atcgcaatac cagcccaata ccaaccaggg tcacaagcgc gcctgctatg
      421 gagttggggg tggggacctg gctgaggaat atccaggaga ggatgatgcc gccggtgact
      481 tcttgcgagg cgatgatgtt gacataagcg gcgtgggtgc ggcgcaggga ggcgttgtac
      541 agagtgtgtc cgagcgcgcc ggagagcagg ccgacggctg cgatagcgcc cagcttggtc
      601 cagtcccacg cttcggggga cattcggggg atggtaaaga gggcgagagg caatagccag
      661 agggtggcgg accagtagac gcgggaggcg tagaccagca gtgggtaccg ttcgcgctcg
      721 tagcgcccgg ctaccgagta gaggccgaac atgacggccg agatgagggc caggaggtcg
      781 ccaaaggcca tctcgaggtc catgtgcggc tcgaggccag caaggattgc cacgcccagt
      841 acggttacgc cgatgcccgc ccattgcctg ctacgtagct gctctttcag gaagagggcc
      901 gaaaagaggg ccacgaagat gggggccgtg tatatgagcg atagggcgtg ggcggcggtg
      961 gtgaagctga gcgaggagat gtaaaagagg aagtgcaggg cggcgaccag gccataagtt
     1021 aggaacctca gcgtgctgtt gccccgcgac ctcgccgcga caccggctgc catgccctcg
     1081 tctcgcgccc ggtctcgcgt gagccaggcg aggccgccca ccgcgagggc cgccacgagc
     1141 atgcggcccc acgtcttgat gaaggggtct atggggtcgg cccacacgat gaacacgggg
     1201 ctggtggaga agaagaacac cgccagcgcg acgtaaagca ggccctgccg ctcgtctcta
     1261 ctcacaggga cggcccgtgg gctggggtct tgccctcctg ggcgcgcagc ttgtccgcga
     1321 ttatgccggg catggcatcg gtccagagct tgtaatatac gttgcgcgcc gtggggaaca
     1381 agcccagcat gcgccggtat gtctgcgaca cctcggacag cttgcgcggg gcgggccagc
     1441 cttgctccag gacggtggga tcgacgtacc gctgcttcat gtgcgtggag aagcaggggc
     1501 cgccgtcgtc cacctcgtaa aagcgggtgc cgggtgtcaa cagggtgagg taagcctgca
     1561 cttcggggtc gtccaggctt cgcaggcgcg acatggcctc ggcgtcggtg gagaagaggt
     1621 ctttgtccgt gaagtcgccg gtttcgtagc cccgccgtat cgcaccggcc agcgcccaat
     1681 aggcacccgc ctcctggggg ccggtccagt agcggttgtt ggcctcgctg aacaggtgag
     1741 cgaaccactc ggcggcctcc gtgtggtcta ccaggatgcc ggagggcgtg ggtagcaggt
     1801 gggagaggaa gtctgccgcg tggtgggggt ctacctggtg gcgggcgcgc aagtcgcgca
     1861 gggtgtagtc gatgcggtcg gcgcagaggc cggggagggg cctttccagg agcgggtagc
     1921 tatcgggttc gagggcggcg tgggggctta cgccgtggcg gcgcagtatg gcgggcacgg
     1981 tggagcgtgt gattacgtcg tgctgaaatc gctcgtggaa gttgtgctcg tcgttgggga
     2041 aaacgatgtc cgcggtgtgg gagaaggcgg tatggggcac gtcgtgcagc agcccggcga
     2101 cgcgctcctc cagcccggca ccgagccgcg aaagcacgtg tagcacgccc aggctgtgct
     2161 cgtagcgggt ggcatgccgc gtgccgggca gcaggtagac gctcgcgccc gcctggtgta
     2221 tgcccttgag ccgctgcacc ggggcggact ccacaagctc cgcgagcacc ggccggtcaa
     2281 ttagtatgtc gccccacagg ctgtcctcgt agctggcaga catgggggca ttataacgta
     2341 ctcaggaggc ggcaggggta acgggaaagc ctgtgtcagc ggggcatccg ggcatctgcc
     2401 ccgcgttcgg gcggtgttag tgtgttgcgg ggcgctcgcg ggcgaacagg gggattgtct
     2461 gcgccaggca cgtgcgggcg aagtacacca gccaggcgac gatacccagc agcttggccc
     2521 cgtcttccag gaggaggcgt atggccggga cgttatcacc gtaggggccg tttagcttgt
     2581 cgaagaccag cgacataccc atgaagccgc aggcgagcag gaacaccagg aactcggttg
     2641 aggcaatcgt tcgccagaac ctcgcgatga agaggacgaa catgaagccg tagagagcga
     2701 acaccgtttc ctcgccgaag cgccagtaga tttgtgaggc gttttcgtgg agcatgaaga
     2761 agtcgtctat cagcagcacc gtggtgacga tgcccgccgc aaagaagaac gcgggccacc
     2821 ggctcgtggc cccggccttt ttgagcacgg tccagctgaa gaggcagacg gcggcgcagg
     2881 agcaccagag cagcaccccc aggccggaga caacgccaac gtagttcggg acgtgcaata
     2941 tgcctgcggg gtcgcgcagg aaataagcca caggcacgtt gctggtgagg ctggccgcga
     3001 atacagctac aagcacaagc aaggcggggg cgtagacgag gagcagggtg gggagcagcg
     3061 tgcgtgcctg cactacgatc ttattgaaag gtgtcgctgc tgaaaggagc ggcaactctc
     3121 tgacggggtt cataagggcc atattcgttc tctctcacta tgcgggggac gtgctcagcg
     3181 accctgtggg tacaggggtc cgcctcagtt tatgccctcg caggcccccg tcacatcacc
     3241 cgaacgcgct aaaggtgtgg tgcgtaaagt agtaggggag gctagtcatt caggtgcgga
     3301 gcgcggtgcg cgttacccga tccggttgtg aggctaacgc acttcgaccg cggtatgttg
     3361 taaggtgcag gtcacggtct caccggaggt catcatgcgg ctgctctgcc agctcgagcg
     3421 ctcatattga cgtgcttgct cgtagcgggt gacctctgtg ggagcgttga acaataagtg
     3481 ccatgatgct gacgttcaac agaggggcac cgggagcagc ttagattctt cgctacgccc
     3541 agaatgacag ggccactctg gtcctgctgc tgcggcactt tgcagatagg cccttgcact
     3601 cgctatcttg aaactgttgt tcttcaggtg cgctataagg ctgccggatt gctaatagac
     3661 atgctaaaat acctcacaat aagtcttgca atcgtcgctt aataggcgta tacttatttc
     3721 gttttaagcc cttgagccgt tactctaata agtagcggct cgttgtggca ctggcacgcc
     3781 tatgccggtg ccactgtatg cgcaccctga agagatgcat aggaggaaaa atgcacggcc
     3841 taatgaacag catatggaac tctcgattca gtcgcacctt tcttgccgtt atacccctgc
     3901 tggtcatccc gcttgtaata agcctgggag tggcccccaa tgcgcgggat acaagtgcgt
     3961 tcgcggcctc gcctactgtg ctgcacgacc agctcaacaa ctttgaaggc accatcgtgt
     4021 cgttcgaaaa cggcaccaac ttcagcagcc aggcggctga tgatttcata gtgcctccgg
     4081 ggaagacctg gacgctcacc caggtggacg tctccggttt ccctgtcgtg ggaagcgacc
     4141 agcctgcatt cttcaacgtg ttcctctaca gagacaatgg taccctaccg ggcgtgcagg
     4201 ttgcgagtta ctccaacctc agctacgcgt tcaactctac gaacggggag tactcgattg
     4261 cttttccgtc taccttgtta aaagctggaa cgtactgggt ctcggtgcag gccgacacca
     4321 cggccgcggg caacaggtgg acgtggacgg gccgcctgac accggcggtg aacaatgggg
     4381 cagcctggcg caatccgggc aacggcttta gctcgggctg caccgactgg ggcaccaaga
     4441 cggcatgcca gtccgcgacg ttttccgggc cagaccagac tttccgcttg catggcactg
     4501 aggagacacc agtgccggct cccgtgctgc acgaccaact caacaaattt gagggcagca
     4561 tcgtgtcgtt tgagaacggc accaacttca gcagccaggc ggctgatgat ttcatggtgc
     4621 ctccggggaa gacctggacg ctcacccagg tggacgtctc cggtttccct gtcgtgggag
     4681 ccgcgcagcc cacatccttc aacgtattct tgtacagcga tagcagcacc ctgccgggca
     4741 gccaggttgc gagttactcc aacctcgact acgcgttcaa cactacgaac ggggagtact
     4801 cgattgacgt gccccccacg tcgcttggta gtggaacgta ctgggtctcg gtgcaggccg
     4861 acaccacggc cgcgggcaac aggtggacgt ggacgggccg tttgacaccg gcggtgaaca
     4921 atggtgcagc ctggcgcaac cctggcaacg gcttcggctc gggctgcacc gattggggcc
     4981 gcaagacgac atgccagccc gcgacgcttt ccgggccaga ccagacttac cgcttacatg
     5041 gcatcgaggc gacaccaaca gctacgccta ccagccctcc agcggctacg gctacaccag
     5101 tgaggggcgt gccgccaggc tttgagccac attacgcatg ccgagccacc aaggatggcg
     5161 ctctgcgaga taccggagtg cttgtagctg tcggtcctcg gccaacgtgc ctgaagaatg
     5221 aggtgttggt agccattctg gtcaatgacg caccttaggc gctggggtat gaacgtggcg
     5281 ctggctttgc gggataacac ccgcaaagcc agtttgattg tgggcactac tcgatgggca
     5341 cccaggcgac aaaagtggcg tatcattccg ccggttcagc tatgtgtttg gacaggcagg
     5401 gttcagcgcc ccgccgaaac cagcgcccac acaagaccct gtgcgtcggg cgtgctgacg
     5461 gcgcgagcga ccgcgccctg agatgcctgg gtagcaccgc cggagctgtc cggcgccagc
     5521 gagtccccta ttgctacagc ccatgtggtc acgcgcacct gggcgaggcc actgactata
     5581 acccgcacgt aggtgttggg cgcggcggca ccctctgccg gtaccagggg ctggttctgc
     5641 tctttggcgc gctcacctgc cgtatcgcgg accagtgagg taccggctac gcctatgacg
     5701 gcatcccctg gcagccccgc gcggcgcacc agtagcaccg gctggccgga cttcgggtcc
     5761 acctcaaccc ctgccgcggc caccaggtct cccagcctga tgctctcgtc gccgctgttg
     5821 cgcgccacat aggcttgcgt gcagcccgtg caatctccgt ccactgtcag gtcccctgag
     5881 acgtaggcgt gtgacgtgac gtccagattg ccgttgacat aggtccagcc gtccgcgtag
     5941 agcgcaatcc agcttgggcc gccgcgaacg taggcgcccc ggtaattttc tgatgtgaac
     6001 tcgcccccga acccattcgt gcccgtgccc tttacccctg ggccggcccc gtcgtgcgag
     6061 ccccacacgc cgattccgta gccacccgta ccctggttcc gccctcgcac gcccgccgag
     6121 tcgcggcccg cgctggtgtt ggccaccatg cccatgatac ccacggcgtc ggtaattggg
     6181 gaactggtca ggccctgcac tcccgtgccc atgtcgctgt agcctagcac accagtgcca
     6241 ttgccacttg ccccggtgac ccccacaccg gcagcagcag tagtgcgtga ataaagcggc
     6301 tggatgtggc tgggcgtgtc tatgtagata ccgggctgct tgcgtgccag gtctacgaac
     6361 ttggtcatct gcgccctggt cacactatta cctgagcgat agtagggccg gttctggccg
     6421 tcgcacggct cgccgggacc accgcagggg tagccgctga tgatgtcctg catatagagg
     6481 ttgttggcat aatcatagaa agggcttccg ggaagcacgt ccgagaagca ctgaccgcct
     6541 gccgggcatg tggcctgtgg tgctgacggg gcagagggtt gtgccgcgat tgggcgggaa
     6601 aagccaaaca acgctaacac gaacacgctg acgagcagca ccacaatagg ctttctgtgt
     6661 gccgggatag ctgcgttcaa agtcatcatt gtcgtagcct cctatggggt gtaacataag
     6721 atgggtgtgg cagcaatggc gtggttgata cccatattat acgcctggtg caaagagagg
     6781 aatggcatgt cttgtgcttc gaccctcgtg atcagagctg ttagaggttg accaggtttt
     6841 gtgcaaccac cagcacagta gcatgatcca gccatgtttc cgcaacaccg gcagcgggcc
     6901 tctctttgct cgtggcccgg ccctgtggcg gcacagcggc aaccagtaaa ataggcacag
     6961 agagtagcgc ggcactcact caacggaggt ctggtcgtgc gagcaatgat agccttgctt
     7021 cggcagcgca actttggtct actctggttt gggggactga tttcttttgt tggggactgg
     7081 attaccctcg tctccctccc gatctacatc tacaatctca ccggatcggt agctgccaca
     7141 ggtgg
//
LOCUS       DASRWZ010000080         4220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_829107, whole genome shotgun sequence.
ACCESSION   DASRWZ010000080 DASRWZ010000000
VERSION     DASRWZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829107"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..3746)
                     /locus_tag="VFH60_01135"
     CDS             complement(105..3746)
                     /locus_tag="VFH60_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6312411.1"
                     /translation="MSLQSQHSHTPPADDSGDEPHMPPGPARDEASRVGTRPDRHEAE
                     ERANLSEERFRLLVEGVRDYAIFMLDPQGIITSWTPGAQRLKGYTPEEALGKHFSIFY
                     TPEDLQTRKPFRGLETARQTGRFEDEGWRMRKDGSRFWANVVITRILYQGELTGFAKI
                     TRDLTERKQAEEELRRSEQLFRQMFDNAPIGMSMVSLDYRFVRANRAFLDMLGYTSEE
                     LAALTFVDITYPPDVAEDLALAQKLFSGQIPSYKLEKRYVTKAGDVIWIELTVTIVRD
                     AEERPLYVLGMVENISERKAAEDTIKRLNEDLERRVVERTTELRAANAELEVEIGERV
                     AVTQENTRLLAQVEQQRQRLDTIIANVPGVVWEAWGVPDAAGQRIDFVSSYVETLLGY
                     SQEEWLETPNFWLSIVHPDDKETAASVARQTFESGKPGTNQFRWVTKDGRVVWVESHS
                     AAATDEHGNPVGMRGVTMDITERKKLEEQVLGQAKALEHEYERLASLVANVKVGLSLS
                     DKENRYILVNDAALEQTGYTREQVIGRRMEEFANISHKKAYQALADSVLVTGEPVHLS
                     ESYLQNETFPEGRYIDSSIVPMRDAEGNITGLLTAAMDVTEKVRARQEIEAQRSLFRT
                     VLETAPIAITLYDRDMRIIEVNSEQLRLSGTTAEQLLGNVIYDLYPVALERKHLHDQV
                     LAGDSVDIHSVPYPHPSGERYYDIRYRPMRDSRGEVVGLLSSSVDVTEQVMARQAIED
                     QRKFLEVIIESTPVAIAFYDPEMHVVDVNGTWQALTGLRLEDIKGRVLYDVNPQAIGR
                     KDLYDRALAGEIYEGEGIENIAPDGEVRFIDAAIRPVYDSEDKLMGLLVTSVDVTDKI
                     RSKDEIEAQRSLLATIIEGMPVGVVYYDTDMRIVNYNAEWERIAGLERTGDMRGLTLY
                     ELDPPTQARRQLHVRALAGEMISRVNVEHVQPRTGKTVYADIHLRPVRDTSGKIVGLL
                     SVIVDSTLRHEIDQAKDSFIALASHELKTPITTIKGYAQSTLRNLDKYDGERLARTLT
                     IINEQSNRITRLVNEMLDVSRMESQTLTLNVEHFDMRAVIEEVASNQELVSPEFELDV
                     HLPEGPLTVGADRQRIEQVLTNLVQNAVKYSGSSRRIEVAAWREGGEVVVSVRDYGVG
                     IPATQQSRVFQRFFRASNVSTSNYSGLGLGLFISHGIITRHDGRMWLESSEGAGSTFY
                     FALPLVGEQ"
     gene            complement(3947..>4220)
                     /locus_tag="VFH60_01140"
     CDS             complement(3947..>4220)
                     /locus_tag="VFH60_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET6312412.1"
                     /translation="TPITGKHVLLVEDVVDTGLTLAYVLKLLRARQPRSLRVCTLLDR
                     ESVRLIDMPLHYVGFPIPDQFVVGYGLDYRQLYRNLPYIGVLKRPD"
BASE COUNT          677 a         1355 c         1294 g          894 t
ORIGIN      
        1 ccgtagtctg agtggcgagt ggttagtggt gagtggcacg cagccaagcc atccttatcc
       61 tttgcgcgtt ctttgcgtct ttgcgttttc gtctcgtcac ttcactattg ctccccaacc
      121 agcggcaggg cgaagtagaa ggtgctacct gcgccctccg acgactcgag ccacatacgg
      181 ccgtcgtggc gcgtgatgat gccgtgcgag atgaacaggc cgaggcccag gccgctatag
      241 ttggaggtgg acacattgct ggcccggaag aagcgctgga acaccctcga ctgctgggtg
      301 gccgggatgc ccaccccgta atcgcgtacc gagaccacta cctcaccacc ctcccgccac
      361 gccgcgacct ctatcctcct gctgctgccc gagtacttga ccgcgttctg caccaggttg
      421 gtcagcacct gctcgatgcg ctggcggtcc gcccctaccg tgaggggtcc ctccggcaga
      481 tgcacgtcca gctcgaactc cggcgacact agctcctggt tgctcgccac ctcctctatc
      541 accgcccgca tgtcgaaatg ctccacgttc agcgtcagcg tctggctctc catgcgcgat
      601 acgtcaagca tctcgttcac cagccgcgtg atgcggttcg actgctcgtt gatgatcgtc
      661 agcgtgcggg ccagccgctc cccgtcgtac ttgtcgaggt tgcgcagggt ggactgtgcg
      721 tagcccttga tcgtggtgat gggcgtcttc agctcgtggg aggcgagggc gatgaacgag
      781 tccttggcct ggtcgatctc gtgccgcaag gtggaatcga caatcacgct cagcaaaccc
      841 acgatcttgc cgctcgtatc ccgcacgggg cgcaggtgta tgtcggcgta tacggtcttg
      901 ccggtgcggg gctggacgtg ctccacgttc acccggctta tcatctctcc cgcgagcgcc
      961 cgcacgtgca actgcctgcg cgcctgcgtg ggggggtcta gctcgtagag cgtgaggccg
     1021 cgcatatcgc cagtgcgctc cagccccgct atgcgctccc actcggcatt gtaattgacg
     1081 atgcgcatat ccgtatcgta gtacaccacg cccaccggca tgccctcgat gatcgtcgcc
     1141 agcaacgagc gttgcgcctc tatctcgtcc ttggagcgta tcttgtccgt cacgtctacc
     1201 gacgtgacca acaggcccat gagcttgtct tccgaatcgt acaccgggcg aatggcggcg
     1261 tctataaacc ttacttcccc atccggggct atgttttcta tgccctcgcc ttcgtagatt
     1321 tcccctgcca gcgcccggtc gtataaatct ttgcgtccga ttgcctgggg gttcacgtcg
     1381 taaagcacgc gccccttgat gtcctccagc cgtagccccg tcagcgcctg ccaggtgccg
     1441 ttcacgtcta cgacgtgcat ctccgggtcg tagaacgcta tcgccacggg cgtgctctcg
     1501 atgatgactt ccaggaactt gcgctggtcc tcgattgcct ggcgcgccat gacctgttcg
     1561 gtcacgtcga cagagctgct caagaggccc acgacctccc ctctgctatc gcgcatgggc
     1621 cggtagcgga tatcatagta gcgctcgccg ctggggtgcg ggtacggtac gctgtggatg
     1681 tctaccgagt cgcccgcaag cacctggtcg tgcaggtgtt tgcgctcgag cgccaccggg
     1741 tagaggtcat aaatgacatt gcccagcaac tgctcggcgg tcgtcccgga caggcgcaat
     1801 tgttccgagt tgacttcaat tatgcgcatg tcgcggtcat acagggttat ggcgatgggc
     1861 gccgtttcca gcacggtgcg gaagaggctg cgctgggcct ctatctcctg gcgggcgcgc
     1921 accttctccg taacgtccat agccgctgtg agcagccccg tgatgttgcc ctcggcgtcg
     1981 cgcataggca caatcgagga gtcgatatag cggccctccg ggaaggtctc gttttggagg
     2041 tacgattcgg agaggtgcac aggctccccg gtaaccagca cgctatcggc gagcgcctgg
     2101 taagccttct tgtgggagat gttggcgaat tcctccatgc ggcggcctat cacctgctcg
     2161 cgcgtgtaac cggtctgctc caacgcggcg tcgttcacca ggatgtaacg gttctctttg
     2221 tccgagagcg acagccccac cttgacgtta gccaccaggc tcgccagccg ctcgtactcg
     2281 tgctccaggg cttttgcctg gccgagcacc tgctcctcga gcttcttgcg ctcggtgatg
     2341 tccatcgtca cgccgcgcat gcccaccggg ttgccatgct cgtccgtcgc agccgccgag
     2401 tgggactcca cccacaccac gcgaccgtcc ttcgtgaccc accggaactg gttggtgccg
     2461 ggcttgccgc tctcgaacgt ctgccgggcc acgctcgccg ccgtttcttt gtcgtcgggg
     2521 tgcacgatgc tcagccagaa gttgggcgtc tcaagccact cttcctgcga atacccaagc
     2581 agggtctcca cgtagctgct gacgaagtcg atgcgctggc ccgccgcgtc gggcactccc
     2641 caggcctccc acaccacgcc gggcacgttg gcgatgatgg tgtcgaggcg ctgccgctgt
     2701 tgctcgacct gggccagcag gcgggtgttc tcctgggtta cggccacgcg ctcgcctatc
     2761 tccacctcaa gctcggcgtt ggcggccctc aattcggtgg tgcgctccac cactcgccgc
     2821 tccaggtctt cgttgagacg ctttatcgta tcctcggccg ccttgcgctc gctgatgttc
     2881 tccaccatgc cgagcacgta aagcggccgc tcctccgcgt cccgcactat cgtgacggtg
     2941 agctctatcc agataacgtc gcccgctttg gtgacgtacc gcttctccag cttgtagctg
     3001 ggtatctgcc cgctgaatag cttctgagcg agggccaggt cctcagcgac atcgggtggg
     3061 taagttatat cgacaaaggt gagggcggcc agctcctccg acgtgtagcc cagcatgtcg
     3121 aggaaagccc tgttggcgcg cacgaacctg tagtcgaggc tgaccatcga catgccgatg
     3181 ggggcgttgt cgaacatctg gcggaaaagc tgctcgctcc ggcgcaactc ctcttccgct
     3241 tgcttgcgct cggtgaggtc gcgcgtaatc ttggcgaagc ccgtcaattc cccctggtat
     3301 aagatgcggg tgatgaccac gttggcccag aagcgcgagc cgtccttgcg catgcgccag
     3361 ccttcgtcct cgaaccggcc ggtctgcctg gcggtttcga gaccgcggaa gggcttacgg
     3421 gtttgcaggt cttcgggggt atagaagata gagaagtgct tgcccagcgc ctcctcaggc
     3481 gtgtagccct tcagccgttg cgcgccgggg gtccaactgg tgattatgcc ctgggggtcg
     3541 agcatgaaga tagcgtagtc gcgcacgcct tccaccagca gccggaagcg ctcttcgctg
     3601 aggttggcgc gctcctcggc ttcgtgccta tccgggcggg tgcctactcg ggaagcctcg
     3661 tcgcgggcgg ggcctggcgg catgtgtggc tcgtctccgc tgtcgtcagc aggtggtgtg
     3721 tggctgtgct gtgattgtag tgacatgccg tcttctttcg tgtgtgaggt caagagtgcc
     3781 ggtacacgcc caaaggcatg gacgagtccc aggtgcagag ggcacaccca aagccacgcc
     3841 cggctatgta gcgcaacagc actcgtattc gcccagaaat ggcagcaata tacatgcctc
     3901 tgtgcgcatt atatagcagc tacagcagct tcagggaggt gcggggtcag tctgggcgct
     3961 tgagcacgcc gatgtagggg aggttgcggt atagctgacg gtagtcgaga ccgtagccca
     4021 ccacgaactg gtcggggatg gggaagccga cgtagtgtag gggcatgtcg atcaggcgca
     4081 cgctttcgcg gtccaacagc gtgcagacgc gcaacgacct gggctggcgg gcgcgcagca
     4141 gcttcagcac gtaggccagc gtgaggccgg tatccaccac gtcttccacc agcagcacgt
     4201 gcttcccggt gatgggcgtg
//
LOCUS       DASRWZ010000081         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_692000, whole genome shotgun sequence.
ACCESSION   DASRWZ010000081 DASRWZ010000000
VERSION     DASRWZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692000"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2828)
                     /locus_tag="VFH60_01145"
     CDS             complement(<1..2828)
                     /locus_tag="VFH60_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312413.1"
                     /translation="MERLQRTPRQERLEVTCRRFAVLSGIRQEADDYIACLQPRSGRK
                     GTLALLTEPAGEHPALSVEACRLARKIIAEQYVADASLSMTSGLLAALDAANNAVLRY
                     NYANALGESNMDSSPLVAVRAGGARARRFKVGLTAALLRTDGTGIYLAQMAPDQAYVR
                     HNGLLTPVPETPHWRARPNSKVNRPQLTILRGDHDALDVTDTDELFHDFSQDNLLSPS
                     LGTSPGVESNPVYRRIEDGDLVVLVTSSLARLLDRETAELVMSGFDADAVTEALLELA
                     IKHGLAQAHACVLAFGGPTSSGIDMQWSGPEIVTVQEDNAEATLAVEPEAVALQAHES
                     EIEGPPLQGAALSRPTFTILEPDATQTRHADLDEDDFRLPWETPSLEVLRPVDAEQVE
                     AEPEEPESPASLHAALRPTRLLVQHQLERPPFHAPILWEDDFEEELGELNFDGWEDSL
                     PASSTLVEGEAETDVPDPEQVVFPWQKETRLQPLAVVSQDAQTETPAITPFPAPDLFG
                     SGPQPYDAAAFDAWGEVEVPKARRFAMPAFSMPRLSLPRISAPKLPRVTMPKAPDLLK
                     AGRGAATWARSLGQNMLPERPFRLPRVASAGGNIGGVPVRLLTALALVLVVGILAWSV
                     FKAVGDNQQAQVNTLLEQAKQEELLANQPGTNEAQRVELLESALQHARQAALAGSDTQ
                     EAQVFADKVQGQLDKLQGITRLDLTLLFQFGQAGGAGEVAGDMQSTTATGNAATEPAD
                     IIVSGNEAFVLDRASDAVYKCNISAKACAKVLGTGEVAGGQSAGKPVSMTLRQGNVLV
                     LDDKLSVYAYNAETGSWSAEQLGDAGKLAVPKDIATYDGNLYLLGAKPGQVLKYFSGK
                     YGAPPDDWIKDPATSEQLSNPVSVAIDGSIYVLLADGKVLAMQGGKVTGSITPKPGPA
                     AAPATDTFTRPETRDLYVLRSAWGTVHRL"
BASE COUNT          489 a          988 c         1000 g          573 t
ORIGIN      
        1 aggcggtgca cggtgcccca ggcgctcctg aggacataca ggtcacgggt ctccgggcgg
       61 gtgaaagtgt cagtggcggg cgcggctgcc ggtccgggct tgggcgtgat actgcccgtc
      121 accttgcccc cttgcatagc caacaccttg ccgtcggcca ggagcacgta aatcgagccg
      181 tcaatcgcga cagagacggg gttggacaac tgctcggagg tcgcggggtc cttgatccag
      241 tcgtcgggag gagcgccgta ctttcctgag aagtatttca gcacctggcc cggctttgcg
      301 ccaagcaagt acaggttgcc gtcgtaggta gctatgtcct tcggcacggc cagcttgccc
      361 gcgtccccta actgctctgc cgaccaacta cccgtctcgg cgttgtacgc gtacaccgac
      421 agcttatcgt ccagcaccag cacgttgccc tggcgaagtg tcatcgacac cggcttgccc
      481 gcgctctggc cgcccgccac ctcgcccgtg ccgagcacct tcgcgcacgc tttggcggag
      541 atgttgcact tgtagacggc atcgctcgcc ctgtccagca cgaaggcctc gttgcccgac
      601 acgatgatgt ccgcaggctc cgtagccgcg ttgcccgtag cggtggtgga ctgcatgtcg
      661 cccgcgactt cccccgctcc gcccgcctgt ccgaattgga acagcagggt caggtcgagc
      721 cgggtaatgc cttgcagctt atccagctgg ccctgtacct tgtcggcgaa cacctgcgct
      781 tcctgcgtgt ccgaccccgc tagggcggcc tgccgtgcgt gctggagggc gctttccagc
      841 agttccacgc gttgcgcttc gttcgtacct ggctgattgg ccaataactc ttcttgctta
      901 gcttgttcca ggagcgtgtt cacctgcgcc tgctggttgt cccctaccgc cttaaagacc
      961 gaccaggcga gtatgcccac caccaatacc agcgcgagcg ccgtgagcaa gcgaacgggc
     1021 acgccgccga tgttgccgcc tgccgaggct acccttggga gcctgaaggg ccgctccggg
     1081 agcatgttct ggcccaggct gcgcgcccac gtcgctgcgc cgcgacccgc cttcagcagg
     1141 tcaggggcct tgggcatcgt gaccctcggg agcttcgggg ccgagatgcg cggtagggag
     1201 agccgtggca tggagaaggc cggcatcgcg aagcggcggg ccttggggac ctcgacttct
     1261 ccccatgcat cgaacgcggc ggcatcgtaa ggttgtggtc cgctgccgaa caggtcgggg
     1321 gcggggaacg gggttatggc cggtgtttca gtctgcgcgt cctgtgaaac gacggcgaga
     1381 ggctggagcc gggtctcctt ctgccagggg aataccacct gctccgggtc aggtacgtct
     1441 gtctcggcct cgccttccac aagtgtgctc gatgcaggca acgagtcctc ccaaccgtca
     1501 aagtttagct cgcccagttc ttcctcgaaa tcgtcctccc acagaatggg cgcgtggaac
     1561 ggcgggcgct ccagctggtg ttgcacgagc aggcgcgtgg gccggagggc ggcgtgcagg
     1621 ctggcagggg attctggctc ctccggctca gcctccacct gctccgcgtc aacagggcgc
     1681 aacacctcca gcgacggtgt ctcccacggc agccggaagt cgtcctcgtc caggtctgca
     1741 tgccgggtct gggtggcatc cggctcgagg atcgtgaaag tggggcggga aagggccgcg
     1801 ccttgcaagg gtgggccttc tatctccgac tcgtgtgcct gtagggccac cgcctctggt
     1861 tctacggcga gtgtggcctc cgcattgtcc tcttgcacgg tgacgatctc cggcccgctc
     1921 cactgcatgt ctatgccgga ggaggtgggg ccgccgaagg caagcacgca ggcgtgggcc
     1981 tgtgccagcc cgtgcttgat tgcaagctca agcagggcct cggtcacggc atcggcatcg
     2041 aacccggaca tgaccagctc ggcggtctcg cggtcgagca atcttgccag gctggacgta
     2101 accagcacca ccaggtcgcc gtcctctata cgcctgtaga cggggttgct ctccacgccg
     2161 ggcgaggtgc ccagcgaagg gctgagcagg ttgtcctggc tgaaatcgtg gaacaactcg
     2221 tccgtatcgg tgacatccag cgcatcgtga tcgccccgca ggatggtgag ttgcggccgg
     2281 ttgaccttgc tgttggggcg ggcgcgccag tggggagttt caggcacggg cgtgagcagg
     2341 ccgttatgcc tgacgtaggc ctggtcgggg gccatctgcg ccaggtagat gccggtgccg
     2401 tcggtgcgga gcagggcggc ggtgaggccc accttgaagc ggcgcgcccg cgcgcccccg
     2461 gcacgcaccg caacgagagg gctggagtcc atgttggact cgcccagggc gttagcgtag
     2521 ttgtagcgga gcacggcgtt gttggcggcg tccagggcgg cgaggaggcc gctggtcata
     2581 ctgagggagg catcggccac gtactgctcg gctatgatct tgcgggccag cctgcacgcc
     2641 tccaccgaga gggcggggtg ctccccggca ggctcggtca gcagggccag cgtgcccttc
     2701 ctgcccgacc tcggctgcag gcacgcgata tagtcgtcgg cctcctgccg gataccgctc
     2761 aagacggcaa acctgcgaca agttacttcc aaccgctcct gacgcggggt acgttgcagt
     2821 cgctccatgc gctccataat gacctctctt tgcgcctaca ggacgccaaa cgtgacgctc
     2881 gccacaatac catgtctatg ccagcgacac gcggccagta tagcaacggg ctagaacata
     2941 agccgggttt tgcacaacga agtaatgagg actgaggact gaggactgag tagttcatca
     3001 accactccct gattctcagc cctcagtcct cagtcctcat tacttcgtcg
//
LOCUS       DASRWZ010000082         1895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_143941, whole genome shotgun sequence.
ACCESSION   DASRWZ010000082 DASRWZ010000000
VERSION     DASRWZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143941"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFH60_01150"
     CDS             complement(<1..469)
                     /locus_tag="VFH60_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6312414.1"
                     /translation="MAGERLRWGVLGTARIADTVVPGILKARNSELVAVASRDGAKAR
                     AWAQARGRELYVASYDEMLASNLIDAVYIPLPNALHAEWSIRAAERGKHVLCEKPIAT
                     HASQVEELIAAQRRSGVKVMEAFMYRFHPQSERVRQLVEGRAIGETRIVRATFD"
     gene            complement(513..1838)
                     /locus_tag="VFH60_01155"
     CDS             complement(513..1838)
                     /locus_tag="VFH60_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET6312415.1"
                     /translation="MSDTVDKYREYVITGFVKAVQPIVIERAKGAIVTDEAGRDYIDC
                     FAGIAVVNAGHCNPEVIAAATEQMEKLVHCASYVYHTKPEAYLTEKIAQITPGRLKKS
                     FFANSGAEAIEGALKLARLYTGKTEVISLTHSFHGRTWAALSVTGNAGRKRRGGPYAS
                     GVAFAPAPYMFRSRAANPEELGEQCAQAIEEIIQYSTSDDVGAFIAEPLLGEGGLIVP
                     PQNYFQAVKRVLDKHGILFIADEVQTGFGRTGAMFAVEHYGVEPDIIVTAKGIADGFP
                     LGAFTTRDEIAAAFRPGDHLSTFGGNPVSCAAALANIKFIEREGLSDKAREDGEYVMG
                     RLRALQEEFPLIGEVRGMGLMIGIELVADEALTPAVAQAEAIRAYCLEQGVLVGVGGV
                     YGNVVRIQPPLVITRHQLDAALGVLWRALTQVTGAEHPGLQPVASTATR"
BASE COUNT          304 a          629 c          586 g          376 t
ORIGIN      
        1 agtcgaacgt ggctcgcact atgcgggtct cgccaatggc tcggccctcg actagctgtc
       61 gcacgcgctc gctctggggg tggaagcggt acatgaaggc ctccatcacc ttgaccccgc
      121 tcctgcgttg ggcggcaatt agctcctcta cctggctcgc gtgggtggcg atgggcttct
      181 cgcacagcac gtgcttgcca cgttcggcgg cgcgtatgct ccactcggcg tgcaaggcgt
      241 taggaagtgg gatgtacacc gcatctatga ggttggaagc cagcatctcg tcatagctgg
      301 cgacatagag ctcccggccg cgagcttgcg cccaggcgcg ggcctttgcc ccgtcgcggc
      361 tggcgactgc caccagctcg ctgttgcggg ccttcaggat acccggtacc acggtgtccg
      421 ctatgcgcgc ggtgcccagt actccccatc gcaacctctc gcctgccata cgtgccaccc
      481 cggtgatgca gaaaacgtcg tagcttggcg cgctatcttg tagcggtgga ggccaccggc
      541 tgcaaaccgg ggtgctccgc gccggttacc tgcgttaggg cacgccagag cactcccagg
      601 gccgcgtcta gctggtgcct ggtgattacc aggggcggct gaatcctcac gacgttgccg
      661 tagaccccgc ccacgcccac caggacgccc tgctccaggc aatacgccct tattgcttcc
      721 gcctgcgcga cggcgggggt gagagcctcg tccgccacca actcgatacc aatcatcagc
      781 cccatgccgc gcacttcccc gatgagcggg aactcctctt gcagcgcgcg gagcctaccc
      841 atgacgtatt cgccatcctc gcgggccttg tcggagagac cttcccgctc tatgaacttg
      901 atgttggcca gggcagccgc gcacgacacc gggttgccac cgaacgtgga caggtggtcg
      961 cccggcctga acgccgccgc gatttcgtcc cgcgtggtga acgccccgag cgggaagccg
     1021 tccgcgatgc ccttggccgt gacgatgatg tcgggttcca cgccgtagtg ctccaccgcg
     1081 aacatagccc cggtgcgccc gaagccggtc tgcacctcgt cagcgatgaa gaggatgccg
     1141 tgcttgtcga gcacgcgctt tactgcctgg aaatagttct gaggcggcac gataagcccg
     1201 ccttctccca gcagcggctc cgcgatgaac gctcccacat cgtccgaggt ggagtactgg
     1261 ataatctcct ctatcgcctg ggcgcactgc tcgccaagct cctccgggtt ggccgcacgc
     1321 gagcggaaca tgtagggagc cggtgcgaaa gccacgcccg aagcataagg tccgccccgg
     1381 cgcttgcggc ccgcgttgcc cgtgacgctg agcgcggccc aggtgcggcc gtggaagctg
     1441 tgggtgagcg atattacctc ggtcttgccg gtgtagagcc tcgccagctt gagcgcgccc
     1501 tctatggctt ccgcgccgct gttggcgaag aagcttttct tgaggcggcc gggggttatc
     1561 tgcgcaatct tctcggtcag gtaggcctca ggtttggtgt ggtagacgta cgaggcgcag
     1621 tggaccagct tctccatctg ctccgtggcc gccgcgataa cctccgggtt gcagtgcccc
     1681 gcgttgacga ccgctatgcc cgcgaagcag tcgatgtagt ctctacccgc ctcgtcggtg
     1741 acgattgccc ccttcgcccg ctcgattacg atcggctgca cggccttcac aaagcccgta
     1801 atgacgtact ctcggtactt atcgactgtg tcgctcattg gtaggctcct tttcaaagta
     1861 atgagtgatg aggactgagg actgagtagt tcatt
//
LOCUS       DASRWZ010000083         5119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1034591, whole genome shotgun sequence.
ACCESSION   DASRWZ010000083 DASRWZ010000000
VERSION     DASRWZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034591"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..669)
                     /locus_tag="VFH60_01160"
     CDS             complement(199..669)
                     /locus_tag="VFH60_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HET6312416.1"
                     /translation="MQAEVQTSRVRTPDAPAAIGPYSQAIVANGMVFCAGQVALDPAT
                     NDLAGHDIQTQTGRALENLRAVLVAAGSDLQYVVRTTVFLSKFSDFTAMNEVYERYFG
                     EVAPARSTIECGLPGHILVQRDCSAIVPSLVSTIQVIEARESRSTFNLDEDADF"
     gene            841..1920
                     /locus_tag="VFH60_01165"
     CDS             841..1920
                     /locus_tag="VFH60_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013693149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsO family NAD(P)H-dependent flavin-containing
                     monooxygenase"
                     /protein_id="HET6312417.1"
                     /translation="MAGNTEPGRYEVVVIGGGQAGLSVGYYLRRSGLRYVILEAQEGP
                     GGAWLHGWRSLTLFSPARWSSLPGWIMPGGATEYPGRDAVLAYMSEYEQRYGLPVARP
                     VRVGAVRARSDGLEVVSDSGIWHGQAVVSATGTWEGPHIPSYPGRELFGGIQLHSAHY
                     ISPEPFRGKRVLVVGGGNSGAQILAELSLPADATWVTRRPPRFMPDDVDGRYLFDLAT
                     RKYLAEQRGDSDSLREVVTTNLGNIVMVPPVREARERGILHSVPPFERFTATGVVWPD
                     GREEDVDAVIWCTGFKPALDHLDPLGIIEPDGHVEVVGTRSAGQPRLWLVGYGDWTGY
                     ASATLIGVGRTARSTVEEITTFLRG"
     gene            complement(2151..3011)
                     /locus_tag="VFH60_01170"
     CDS             complement(2151..3011)
                     /locus_tag="VFH60_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET6312418.1"
                     /translation="MTTERGSSAHSDAEAGQEGCLFGGIQLDRETCEFYRSAVVLLTE
                     EEIPFLVGGAFAFQCYTGISRFTKDFDIFVKPEDVERTLELLGQNGCKTEMTFSHWLG
                     KAYCGDLFVDVIFRSGNGVSEVDDEWFDHATPGEVLGIPVKLCPPEEIIWTKSFVQER
                     ERYDGADIAHMVRASSESMDWKRLVRRFGPHWRVLLSHLVLFGFVYPGERDKVPAWVM
                     NELMGKLQHELATPAPKTRLIRGTLISRQQYLIDIEEWGYKDARLEPKIKMTEDEIEA
                     WTEGIEVDGS"
     gene            3196..3981
                     /locus_tag="VFH60_01175"
     CDS             3196..3981
                     /locus_tag="VFH60_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6312419.1"
                     /translation="MPKDKRKVRIAAVGDIHCNRSSQGAFQPLFSEINEKADVLVLCG
                     DLTDYGLPEEAHVLVRELAVLRVPVLAVLGNHDFESGKQDEVTGILGDAGVLMLDGDA
                     QEVAGVGFAGAKGFAGGFGTGTLAPWGEAAVKAFVQEAVNEAMKLEKAVARLRTPHKI
                     VVLHYAPIQTTVEGEPEAIFPFLGSSRLEEPLARYPVSAVFHGHAHRGTLEGRTMSGS
                     PVYNVAMPLLKRDFPNKPPFYLFEVGDEGTSDMPVDGRVRAEV"
     gene            complement(4258..4563)
                     /locus_tag="VFH60_01180"
     CDS             complement(4258..4563)
                     /locus_tag="VFH60_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312420.1"
                     /translation="MTDRTNDAGKAQRPSASHLFLVRLWEEPADGTSHAGHVADASGA
                     QPGAVGASDREWKGKVQHVVSGESHTFQDLPSLLDYLRAMMPASDPGTPGNGPATTE"
     gene            complement(4724..>5119)
                     /locus_tag="VFH60_01185"
     CDS             complement(4724..>5119)
                     /locus_tag="VFH60_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312421.1"
                     /translation="NDIRAAGSARAKPRWQTEAIEEYEESLQPLVVRLRRDMASRVLA
                     LTGHRVSPDAIYADSANRVAVTTVDGVVFKLIKENLVVLRPCVQCGCCQFQSMPITNA
                     AELGYALSEWEPRCPGCIPQDSDCWLYTD"
BASE COUNT          891 a         1481 c         1685 g         1062 t
ORIGIN      
        1 accactgtga taaccggctt gatccaccag ttgtccctcc gtgcgttgag gggcagcggc
       61 gctcgcccag cttccttgct ttgcgcggtg cgtacagcca tgtagcatca ctcctggtcg
      121 gtaaaggtgg tggtttggcc ccggaccttt tacacagaat tgccggtagt cgagattata
      181 gcacggagcg tgcggatgct aaaagtccgc gtcttcgtcg aggttgaacg tggagcggga
      241 ttcgcgggcc tctatcacct ggatggtgct gacgaggctc ggcacgatgg cgctgcaatc
      301 tctctgcacc aggatgtgcc ccggcagccc gcactctatc gtggagcggg cgggggcgac
      361 ctccccgaag taacgctcgt agacctcgtt catcgcggtg aagtcgctga atttcgacag
      421 gaagacggtg gtgcgcacta cgtactgcag gtcggacccg gcggctacga ggacggcccg
      481 caggttctcc agggcgcggc cggtctgggt ctggatgtcg tggcctgcaa ggtcgttggt
      541 ggcggggtcg agggcgacct gcccggcgca aaagaccatg ccgttggcca caatagcctg
      601 tgagtatggg ccgatagccg ccggagcatc aggcgtcctg acgcggcttg tctgtacctc
      661 tgcttgcatc tcttgccctc ctcgttgctg gcgctagata cctggtacta tggcggaatg
      721 ttattctacc gaccggcatt tgtcaatagc tgtggtgtta gcgaccggtc atgagcaggg
      781 cctgtattct gtgtattatt aaagcgtgat ggatgcatga tagcgggtgg ggaagcacgt
      841 atggcgggaa acactgaacc gggaagatac gaggtcgtgg tcataggcgg cggacaggcg
      901 gggctgtcgg tgggatatta cctgcggcgg tcggggctgc gctacgtgat actggaggcg
      961 caggaggggc ctggtggggc ctggctgcac ggctggcgct cgctcaccct gttctcgccc
     1021 gcccgctgga gttcgctacc tggctggata atgcccggtg gggccaccga gtaccccggt
     1081 cgcgacgcgg tgctcgcgta tatgtcggag tatgagcaac gctatggcct gccggtggcc
     1141 aggccggtcc gcgtcggcgc ggtgcgtgcg aggtccgacg gcctggaggt ggtaagcgat
     1201 agcggcatct ggcatggaca ggccgtggtg agcgccactg ggacgtggga ggggccgcat
     1261 atcccatcgt atccggggcg cgagctattc ggaggcatac agctacactc ggcccactac
     1321 atatcccctg agccgtttag aggcaagagg gtgctggtcg tgggcggggg caactcgggc
     1381 gcgcaaatac tggcggagct gtccctgccg gcggacgcga cgtgggtgac aaggcggccg
     1441 ccccgcttca tgcccgacga cgtggacggc cgctacctgt tcgacctggc tacgcgcaag
     1501 tacctggccg agcagcgggg cgacagcgac tcgctccgcg aggttgtgac caccaacctg
     1561 ggcaatatcg taatggtgcc cccggtgagg gaggcccgcg agcgtggcat actccatagc
     1621 gtgccgccct tcgagcgctt cacggcaacg ggtgtggtgt ggccggacgg gcgcgaggag
     1681 gacgtagacg cggtaatctg gtgcacgggc ttcaaaccgg cgctcgacca tctggacccg
     1741 cttggcatta tcgagcctga cgggcatgtt gaggtggtag gcactcgctc ggccggtcag
     1801 ccccggttat ggctggtggg ttacggcgac tggacgggct atgcctcggc tacgctgatt
     1861 ggtgtgggtc gcaccgcacg ctcgacggtg gaggagataa cgactttctt gcgggggtaa
     1921 agacggtgga tgttagaggg cctgtgtggt gccgtgggat agtcctgtgg gggacgccct
     1981 gtggctaaag ccacgggctg ggaagtagca aagtctcctt cggagactac tacatgttcc
     2041 tcagtccccg caggggactt tgcctcttgt aagcccgtgg ctttagccac aggggtgtcg
     2101 cagggcaaag ccacagggcg ctacgttctc catcgcctgc tagctgcacc ctaacttccg
     2161 tctacttcga tgccctcggt ccaggcttct atctcgtctt cggtcatttt gatcttgggt
     2221 tccaggcggg cgtccttgta gccccattcc tcgatgtcga taaggtactg ctgcctggag
     2281 atgagcgtgc cccgaatgag gcgagtctta ggagcgggtg tggctagttc gtgttgcagc
     2341 ttgcccatta gctcgttcat cacccaggcg ggcactttgt cgcgctcgcc ggggtagacg
     2401 aagccgaaga gcaccaggtg ggagagtagc accctccaat gcggcccgaa gcggcggacc
     2461 aggcgcttcc aatccatgct ctcggacgac gcgcggacca tgtgcgctat gtcggctcca
     2521 tcgtaccgct cgcgctcctg cacgaacgac ttggtccaga tgatctcctc cggcgggcac
     2581 agcttcacgg gtatgcccag cacctcgccg ggagtggcgt ggtcgaacca ttcgtcgtcc
     2641 acctccgaca cgccgttgcc cgacctgaaa atcacgtcca cgaacaggtc gccgcagtat
     2701 gccttgccga gccagtggga gaaagtcatc tcggtcttgc agccgttctg gcccagcaac
     2761 tcaagggtgc gctcaacgtc ctcaggtttc acgaagatat cgaagtcctt ggtgaacctg
     2821 gaaatccccg tgtaacactg aaaagcaaat gcgccgccga ccaggaaggg tatttcctcc
     2881 tcggtgagga gtacgaccgc gctccggtag aactcgcagg tctcccggtc gagttgaatg
     2941 ccgccgaaaa ggcaaccttc ctgcccggcc tcagcgtcgc tatgtgcgga acttccccgc
     3001 tctgttgtca cttcttctga ttccgtcgca cccacatcgt tctcccgtgt atggactctc
     3061 agcccgctgt cgttgatatg cccgcacatg taaagcagaa gatgtgccat gtagctactc
     3121 aaaagccgcg tggggtatgc attttgctaa ccacgctacg ttgtaaaggt aagtgcattt
     3181 cgcgtggagg aaagcatgcc gaaggacaag cgcaaagttc gcatcgcggc cgtgggagac
     3241 attcactgca acagaagctc gcagggggct tttcagccgc tcttctccga gatcaacgag
     3301 aaagccgacg tgctggtgct gtgcggcgac cttaccgact acgggttgcc cgaggaggcc
     3361 cacgtgctgg tgcgcgagct ggcggtcttg agggtgccgg tgctggcggt gctcggcaac
     3421 cacgatttcg aaagcggcaa gcaggacgaa gtgaccggca ttttgggcga tgcgggcgtg
     3481 ctcatgctgg atggggacgc gcaagaggtg gcaggcgtgg gtttcgcggg cgccaagggc
     3541 ttcgcggggg gcttcggcac cggcacgctg gccccgtggg gcgaggcggc ggtcaaggcg
     3601 ttcgtgcagg aagcggtcaa cgaggcgatg aagcttgaaa aggcagtggc gcgcctgcgt
     3661 acgccccaca agatcgtggt gctgcactac gcgcccatcc agaccactgt ggagggcgag
     3721 ccggaggcca tcttcccatt cctgggttcg agcaggcttg aggagccgct ggcccgctac
     3781 cccgtttcgg cggtgttcca cggccacgcc cacaggggca cactcgaagg ccgcaccatg
     3841 agcggctcgc ccgtctacaa cgtggctatg cccctgctca agcgggactt ccccaacaag
     3901 ccacccttct acctcttcga ggtcggggac gaaggcactt cggacatgcc cgtcgatggg
     3961 agggtcaggg ctgaagtata gcaggagacg gaaccgccaa ggacgccaag gttacgccaa
     4021 gatactaaag tacggttcac cgcagagacg cagaggacgc tgaggttacg ccaagattaa
     4081 ggatggtgtt ccaagcaaaa ggatcagggt acatcatgtc gtaccctgat ccttttgtac
     4141 tcaccataca accctaatct ttgcgtgttc tttgcgtcct ttgcgtcttt gcgttttcgt
     4201 actcttggcg cgctcttggc gtccttggcg tcttggcggt tccgtcctgg ccgcgcatta
     4261 ttcggtggtg gcagggccgt tgccgggggt gcctgggtcg gatgcgggca tcatcgcgcg
     4321 caagtagtcg agcagcgatg gcaggtcctg gaaggtgtgc gactcgccgc tgacgacgtg
     4381 ttgcaccttg cctttccatt cgcggtcgga ggcccccacg gcacctggtt gagcgccgct
     4441 tgcgtccgcg acatgccctg catgggaggt accgtcggca ggctcctccc acaggcgcac
     4501 caggaagagg tgcgatgccg agggccgctg ggccttaccc gcgtcgtttg tacgatctgt
     4561 catagtgata cctgccgttc gaatattgtt gctgacacca gattggtatt gtgttgcgtt
     4621 catggtttgt aatgtgcctc tgatatatcc tgttgttctt ttgctgtgcg ttcgtcttca
     4681 tttatggccg agtatcttta gtgcggagta ctttttcaag ctattagtct gtgtacagcc
     4741 agcagtccga gtcttggggg atgcagccgg ggcagcgagg ctcccactcg gagagcgcgt
     4801 agcccaactc ggcagcgttg gtgatgggca tgctctggaa ctggcagcag ccgcactgca
     4861 cgcagggccg caggaccacc aggttctctt ttatcagctt gaacaccacg ccgtccaccg
     4921 tcgtcacggc taccctgtta gccgagtcgg catagatagc gtcgggggat actctgtgcc
     4981 cggtgagggc cagcacccgc gaggccatgt cgcggcgcag cctcacgacg agcggttgaa
     5041 ggctctcctc gtactcctct atggcctcgg tctgccaccg gggctttgcc cgggctgaac
     5101 cggcggcgcg tatatcatt
//
LOCUS       DASRWZ010000084         1651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1034811, whole genome shotgun sequence.
ACCESSION   DASRWZ010000084 DASRWZ010000000
VERSION     DASRWZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034811"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /locus_tag="VFH60_01190"
     CDS             <1..1517
                     /locus_tag="VFH60_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312422.1"
                     /translation="EDIPSLPPGAAAVAWSPDGTKIAAGWPGRILHISGGPGSEVMVE
                     QFKGDGQGKFVVAGGWAPDNRHLAMTDGIDYGVLDMQTGEFVTVARSFRQPGIARPGV
                     SWSAGIRKVAFAINSTAQDGQRLFLADLDGRNPQLILSHPRPARYTGQVSDYGPPFFS
                     SDSKTMLLRVSRTNTERGQLVYTHESWLVRTDGSGGEKLVDGFNANWRPKPRLDLPMP
                     GFWFAWRTVDLPVANQQAARPYLWGPTAVYSGTETFAEAPGGKMSVVYFDKGRMEIVN
                     PSPPQPTRYLVTAGALAKELVTGQIATGANSYEQRTPADVLVAGDNPAADAPTYATFN
                     KLAAGTDAGKAQDRTGQPIEAVLAKDGTITQDAGLGGTAKVGEFFPQTGHNVPEVFMS
                     YFKGQPWDWVYIAGYPISEPYWTRTEVAGQPRNVLVQVFERRTLTYDPAEVQQYQVQF
                     GNTGLQYYNWRYVQPPSARPAEPTHAPLPEAQPEPTQQRPPADVIPPQPTAVLR"
BASE COUNT          308 a          558 c          541 g          244 t
ORIGIN      
        1 gtgaggacat accgtcgctg ccgccggggg ccgccgcggt cgcctggtcg cccgacggca
       61 ccaagatcgc cgccgggtgg ccggggcgca tcctgcacat ctcaggcggc ccaggctcgg
      121 aagtgatggt cgagcagttc aagggtgacg gccagggtaa gttcgtggtg gcgggcggct
      181 gggcacccga caaccggcac ctcgcgatga ccgacggtat cgattacggc gtgctcgaca
      241 tgcagacggg cgagttcgtg acggtagccc gctcgttcag gcagccaggc atcgcccggc
      301 ccggcgtctc gtggtcggcg gggatacgca aggtggcctt cgccatcaac agcaccgcgc
      361 aggacgggca gcgcctcttc ctggccgacc tcgatgggcg caacccgcag cttatcctct
      421 cgcacccccg cccggcgcgc tacaccggcc aggtgagcga ctatggcccg cctttcttct
      481 cctccgacag caagacgatg ctcctgcgcg tgtcccgcac caacacggag cgggggcaac
      541 tggtctacac gcacgagtcg tggctggtgc gcaccgacgg ctcaggaggc gagaagctgg
      601 tggacggctt caacgccaac tggcggccca agccccggct cgatttgccc atgccgggct
      661 tctggttcgc ctggcgcacg gtagacctgc ccgttgccaa ccagcaggcc gcccgcccct
      721 acctgtgggg ccccaccgct gtctactcgg gcaccgagac cttcgcggag gcccccggcg
      781 gtaagatgtc agtggtctac ttcgacaagg gccgcatgga gatcgtgaat ccttcgccgc
      841 cgcagcccac ccgctacctc gtgacggcgg gcgcgctcgc caaggagctt gtcacggggc
      901 agatagccac gggcgccaac agctacgagc agcgcacccc cgccgacgtg ctggtggcgg
      961 gtgacaaccc ggcagcggat gcgcccacct acgccacttt caacaagcta gcggcgggca
     1021 ccgacgcggg caaagcccag gaccggaccg ggcagcctat cgaggcggtg ctcgcgaagg
     1081 acggcacgat aacgcaggac gccgggttgg gtggcacggc aaaggtaggc gagttcttcc
     1141 cccagaccgg gcataacgta cccgaagtgt tcatgagcta cttcaagggg cagccgtggg
     1201 actgggtata catcgcgggc taccccatct cggagccgta ctggacgcgc accgaggtag
     1261 cgggccaacc gcgcaacgtg ctcgtgcagg tgttcgagcg ccgcacgctc acgtacgacc
     1321 ccgccgaggt gcagcagtac caggtgcagt tcggcaacac cggcctgcaa tactacaact
     1381 ggcgctacgt ccagccgccg tcggccaggc ccgccgaacc gacacatgcg ccgctacctg
     1441 aggcccaacc tgagcctacg caacagcggc caccggcgga cgtgataccg ccacagccaa
     1501 cagccgttct gcggtaaccg gctgctactc ataacgctag gcgttccaga aattccacta
     1561 gtggaagata gttccaggtg gaagatagtt ccaggtggaa gatagttcca ggtggaagat
     1621 agttccaggt ggaagatagt tccaggtgga a
//
LOCUS       DASRWZ010000085         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_75550,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000085 DASRWZ010000000
VERSION     DASRWZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75550"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1157)
                     /gene="argC"
                     /locus_tag="VFH60_01195"
     CDS             complement(87..1157)
                     /gene="argC"
                     /locus_tag="VFH60_01195"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET6312423.1"
                     /translation="MRGMQISGNPVRVAVMGASGYIGGEALRLLLGHPCVEVEAVTSP
                     SHAGKPVTEVHPHLHKLTDLHFTADIRAEEAAGYDAIFMALPHGAAMQRVPEMLAAGG
                     GGGPHGDGPILLDMSGDFRIKDAAQFARFYGQEHTANELATTAAYGLTEWNREAIKAA
                     RLVACPGCFPTGSLLALTPLARAGLLDGNVVIDSKTGSSGSGKEPGEGTHHPVRSQDF
                     RAYNLLKHRHQPEIAQELARWRGDAAGCMKVVFTPHSAPRVRGIFTTAYAFPSRTISQ
                     GELQEIYREAYEQEHFVRLVDNPRVAVVAHSNFCDISATTDGEGAIIVTSAIDNLVKG
                     GAGQAVQDLNLMLGLPEGAGLE"
     gene            complement(1157..2209)
                     /locus_tag="VFH60_01200"
     CDS             complement(1157..2209)
                     /locus_tag="VFH60_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312424.1"
                     /translation="MQITLDKIASVTSRLGLGSKVEVSEYVPAERGTALVVEALEEKA
                     VYGELELTGGRLARIIKGDLIAGVLGERQALKGFVGSVPSHIEPGDTLHILNMGGVIG
                     ICSSANSDFGKPLQVRVIGSVVVGGVPANIADNAVPWQTSLPDCAPIILVSGTCMNAG
                     KTTAACEVVRVLSSRGYRVAAAKLAGVATQRDLLNMQDHGAVCALSFSDAGLPSTTRT
                     DSCLVPAAKGVLSALSACNPDVIVVEFGDGIMGHYGVDILLRDRELMGHVRAHILCAN
                     DLVAAWGGLRYLDELGVSVDCVSGPATDNSAGVDYITQNFNIAAANGRGEPARLAGMV
                     EKLVFAEQGVLAEVVA"
BASE COUNT          375 a          789 c          764 g          407 t
ORIGIN      
        1 cgggcagggc ggtcgggtcc agctcgaccg cgtgctcggg gcgctcgacc cagcgcgagc
       61 gaaagaagcc gctcattcgg tcctgctcac tcgagccccg cgccctcggg caggccgagc
      121 atcaggttga ggtcctgcac cgcctggccc gcgccgcctt tcaccaggtt atctatggca
      181 ctggtgacga tgatagcgcc ctcaccatcg gtggtcgcgg atatatcgca gaagttggag
      241 tgggccacca ctgccaccct ggggttgtcc accaggcgaa cgaagtgctc ctgctcgtat
      301 gcctcccggt agatctcctg caactcaccc tggctaatcg tgcgagaagg gaaggcgtaa
      361 gcggtggtga agatgcccct caccctaggg gccgagtggg gcgtgaatac caccttcatg
      421 caacccgccg catcgccgcg ccacctggcg agttcctggg ctatctccgg ctggtggcgg
      481 tgcttgagga ggttgtaggc gcggaagtcc tgcgagcgta cggggtggtg ggtgccctcg
      541 cccggctctt tgcccgaacc tgaggagccg gtcttggagt cgatgaccac gttgccgtcg
      601 agcagccctg ccctggcgag gggcgtcagc gccagcagcg agccggtggg gaagcagccg
      661 gggcaggcca ccaaccgcgc cgcctttatc gcctcccggt tccactcggt cagcccgtag
      721 gccgccgtcg tagccagttc gttagccgtg tgctcctggc cgtagaacct ggcgaactgg
      781 gcagcgtcct tgatgcggaa gtcgccgctc atatcgagca ggatgggacc gtcgccgtga
      841 gggccgcccc cgcctcctgc cgccagcatc tcaggcacgc gctgcatggc ggcaccgtgg
      901 gggagggcca tgaatatcgc gtcgtacccg gcggcttctt cggcccggat atcggcggtg
      961 aaatggaggt ctgtcagctt gtgcaggtgt gggtgcacct cggtcacggg cttacccgcg
     1021 tgcgacgggg aagtgaccgc ctccacctct acacacgggt ggcccaatag caggcgcaac
     1081 gcctccccgc cgatgtagcc cgatgcaccc atgaccgcca cgcgcacagg gttgccgcta
     1141 atttgcatcc ctcgcatcag gctaccacct ctgccagcac gccctgctcg gcgaacacca
     1201 gtttttcgac catgcccgcc aatcgcgcgg gttcgccgcg cccgttggcg gcggctatgt
     1261 tgaagttctg ggtgatgtaa tcgaccccgg cgctattgtc ggtggcgggg cctgacacgc
     1321 agtccacgct cacgcccaac tcgtccaggt agcgcaggcc gccccacgcg gctacgaggt
     1381 cgttggcgca caggatgtgg gcgcggacgt ggcccatcag ctcgcggtcg cgcagcagga
     1441 tgtccacgcc gtagtggccc atgatgccgt cgccgaactc cacgacgata acgtcggggt
     1501 tgcaggcgct gagggccgag agcaccccct tggcggcggg cacgaggcag ctatcggtgc
     1561 gggtggtgga ggggaggccc gcgtcgctga aggagagggc gcacaccgcc ccgtggtcct
     1621 gcatgttgag caggtcgcgc tgggtagcca ccccggccag cttggcggcg gcgacccggt
     1681 acccccggct cgatagcacg cgcacgacct cgcatgcggc ggtggtcttg cccgcgttca
     1741 tgcaggtgcc ggacaccagg attatggggg cgcagtcggg gagggaggtc tgccagggca
     1801 cggcattgtc tgctatgttg gcgggcaccc cgcccacgac caccgagcct atcacgcgca
     1861 cctgtagcgg cttgccgaag tcggagttgg ccgagctgca tatgccaatg acgccgccca
     1921 tgttgaggat gtggagggtg tcgcccggct cgatgtggga gggcacggag cctacgaagc
     1981 ccttgagggc ctgccgctcg cccagcaccc cggcgatgag gtcgcccttg atgatgcgcg
     2041 ccagcctgcc gcccgtaagc tcaagctccc cgtacaccgc cttctcttcc agcgcctcga
     2101 ccaccagcgc ggtcccccgc tcggcgggca cgtactcact cacctcaacc ttgctgccca
     2161 ggcccaaccg gctcgtgacc gaagcgattt tatccaacgt tatctgcatc gtttttctcc
     2221 ttgcgcccct taccctgtta caattacgaa ctaccgggcg ctcggtactt ggtactcgat
     2281 acacgggact tttttggcaa acaaaaaact cccgccccaa gcaagggacg agagc
//
LOCUS       DASRWZ010000086         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_7176,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000086 DASRWZ010000000
VERSION     DASRWZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7176"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..1224)
                     /locus_tag="VFH60_01205"
     CDS             complement(307..1224)
                     /locus_tag="VFH60_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6312425.1"
                     /translation="MGKIQFGLVIPSGSRDKALSGAYMDNVRKLTELGSGHYDSAWFV
                     DHLQFDDAELMEGWTAVTYLAALNPQFSWGHAVLCQSFRNPALLGKMAATLQYMSGGR
                     FILGLGAGWKEDEYRSYGYDYPPAGTRVEELEETLQIVKALWTEERATFQGKHYRVEE
                     AWCEPKPNPLPTIMVGGSKPKMLRLIARHADWWNVSWTAIEDYRPQVEECERACADVG
                     RDPSTLRRTWFGGSAVGSTEEEVEELAGGRLKAGTAFVGTPQQVIEQMLPFVELGVDY
                     FMLHCGGFPRLTTAELLINEVMPALNAGR"
     gene            1366..>2471
                     /locus_tag="VFH60_01210"
     CDS             1366..>2471
                     /locus_tag="VFH60_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HET6312426.1"
                     /translation="MLNAQVSATIDSEWVRSQFPSLGLEVDGRPVVYLDNPAGTQVPA
                     RTMEAITSYLSAANANVHGAFLTSRRTDAMLAEARQGMAAFLGAASPREIAFGPNMTT
                     LTYAVSRAIGRDLNPGDELIVTELDHDANVSPWRDLAERGVAVRRVPVHLDCTLDMEA
                     LGNLLSPRTRLVAVTHASNAVGTVPDLPRIVEMAHTVGALVWVDAVHYGPHGPIDVQA
                     LDVDYLVCSSYKFFGPHLGILYGKEHLLERLRPYQLRPAPHTVPEKFETGTKNHECIA
                     GLAATLAYLEELGRRTGGLGTAGGQREALMSAMTGIREYEKGLGAALLEGLLSVPGLR
                     IYGITDPDRLDERVPTFCFTIDGQATPEIGRKLG"
BASE COUNT          443 a          774 c          809 g          445 t
ORIGIN      
        1 acggtcgtgt cggcgcgggc gggcagaacc aagcacccct ctgtcatcct gagcgtagcg
       61 aaggacccaa ggaagccact attgtgaggc tgttgaacat agagggttat agtgccgacg
      121 ttcaatgtag aggctatgcc agcatctgag attcttcact gcgttcagaa tgacaggggc
      181 cactttggtc ctgccgttgg acattgaggc ggccgcgctg ggatagtgat aatcttattc
      241 agtttaagca gaggggaggc ccaccagcct cccctctgcc cacgtctaca ctggaagccc
      301 gcctgtttaa cgcccggcgt tgagcgcggg cattacctcg ttgatgagta gttcggcggt
      361 ggtgaggcgc gggaagccgc cgcagtggag catgaagtag tccacgccaa gctctacgaa
      421 ggggagcatc tgctctatca cctgttgggg ggtgcccacg aaggcggtac ccgctttcag
      481 cctgcccccg gcgagttcct ccacttcctc ctcagtagag cctaccgcag agccgccgaa
      541 ccatgtgcgg cgcagggtag aggggtcgcg gccaacgtcg gcgcaggccc gctcgcattc
      601 ctcgacctgc gggcggtagt cctcgatggc ggtccaggag acgttccacc aatcggcgtg
      661 cctggcgatg agcctcagca tcttcggctt ggagccgccg accatgatgg tgggcagggg
      721 gttgggctta ggctcgcacc aggcctcctc cacgcgatag tgcttgccct ggaacgtggc
      781 gcgctcttcg gtccagagcg ccttgacgat ctgtaacgtc tcctcaagct cctccacgcg
      841 ggtgcccgca ggcgggtagt cgtagccgta ggagcggtac tcgtcttcct tccagcccgc
      901 gcccagtccc aggatgaagc ggccgccgct catatactgg agggtagcgg ccatcttgcc
      961 gagcagcgcg gggttgcgga acgactggca gagcaccgcg tggccccaac tgaactgcgg
     1021 gttcagcgca gccaggtagg tgaccgccgt ccagccctcc atcagctcgg catcgtcgaa
     1081 ctgcaggtgg tccacgaacc aggccgagtc gtagtgcccg ctgccaagct cggtcagctt
     1141 gcgcacgttg tccatgtaag ccccgctcaa cgccttgtcc cgcgagccgc tgggtatcac
     1201 caggccgaat tgtatcttgc ccatgctata actactcctt gcgcttgaat gtcgatctag
     1261 ctgtcccgct cgtctgtgtt atagtgtcct tgcatagctt agcaatccgg gtgcccctgg
     1321 aaaattcgca atccgcaatt cgaactttga aattggagag gtcccatgtt gaacgcgcag
     1381 gtatcagcca ccatagatag cgagtgggtg cggtcgcagt ttccctcgct ggggctggaa
     1441 gtagacggta ggccggtggt ctacctggat aacccggcgg gcacgcaggt gcctgcgagg
     1501 acgatggagg ccatcacctc ctacctgagt gccgccaatg ccaacgtgca cggggccttt
     1561 ctgaccagcc ggcgcaccga cgccatgctc gccgaagcca ggcaaggtat ggcggccttc
     1621 ctgggagccg cgtcgcccag ggagatcgcc ttcggcccca acatgaccac cctcacctat
     1681 gcagtcagcc gggcgatagg cagggacctc aatcccggcg acgaactgat agtcaccgag
     1741 ctggaccacg atgccaacgt cagcccctgg cgcgacctgg cggagcgtgg ggtagcggtc
     1801 aggcgggtgc ccgtgcacct cgactgcacg ctcgacatgg aggcgttagg caacctgctg
     1861 tcgccgcgca cgaggctggt agcggtgacg cacgcttcca acgcggtagg caccgtgccc
     1921 gacctgccca ggatcgtgga gatggcgcac acggtgggtg ccctggtgtg ggtggacgcg
     1981 gtgcactacg ggcctcacgg gcctatcgac gtgcaggcgc tggacgtgga ctacctggtc
     2041 tgctcgtcgt acaagttctt cggtccgcac ctgggcatac tgtacggcaa ggagcacctg
     2101 ctggagaggc tgcggccata ccagctacgg cccgccccgc acacggtgcc ggagaagttc
     2161 gagacgggca ccaagaacca cgagtgcata gcgggtctgg cggcgaccct cgcgtacctt
     2221 gaggagctag gcaggcgcac gggcggtctg ggcacggcgg gtgggcagcg ggaggccctt
     2281 atgtcggcca tgaccggcat acgcgagtac gagaagggcc tgggcgccgc gctcctggag
     2341 ggcctgctaa gcgtgccggg cctgcgcatc tacggcatca cggacccgga ccgcctagac
     2401 gagcgggtgc cgaccttctg tttcacgata gacgggcagg ctacccccga aatcgggcgc
     2461 aagctgggcg a
//
LOCUS       DASRWZ010000087         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_692947, whole genome shotgun sequence.
ACCESSION   DASRWZ010000087 DASRWZ010000000
VERSION     DASRWZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692947"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFH60_01215"
     CDS             complement(<1..676)
                     /locus_tag="VFH60_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312427.1"
                     /translation="MAQGGQAGGGQVNVTAPPPRKNNTPLIVGAALAGLLLLGLILWL
                     LFRPRDVNVNVPAPTPTATTQVVEVVVTPTPEPPSPTPVVQTVVVAVTDTPEPPTATP
                     VVQTVVVVVTDTPGPPEPTPTAQVVVVTTTPNPSPTTPPQPTQPPATPTTVPTLTAQE
                     SQIAAPLRQAPEGDQVTYVRYDGSNIQVYFNIAPRETLPEAALQAKQQIKDLLQQLVQ
                     VELTYGQ"
     gene            complement(859..1293)
                     /locus_tag="VFH60_01220"
     CDS             complement(859..1293)
                     /locus_tag="VFH60_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312428.1"
                     /translation="MSAERPRDIGSIVTRAFGEASGCLRGCSGELVVRALFLLGAGAV
                     GLGMRLLIQYMGVPLTLASLFVLTVILLEFWPRAPHPPRSRRRWLYYGPIAGLIVSVC
                     YALYLGAQDSEDVGWWALVAGGMLVLLGLAAKIDRWLKGETA"
     gene            1511..>3710
                     /gene="alaS"
                     /locus_tag="VFH60_01225"
     CDS             1511..>3710
                     /gene="alaS"
                     /locus_tag="VFH60_01225"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET6312429.1"
                     /translation="MTRIDDPIHNPQSEFPNPPYMSSREIRERFIRYFVEKVGSTHVP
                     SSSIVPTNDPTLLFTTAGMVQFKDVFLGNETRPYKRAVTSQKVMRAGGKHNDLENVGP
                     SIRHQTFFEMLGNFSFGDYFKREAIAFAWELLTSPQEQGGFGLDPNRLWPTIYLDDDE
                     SFVLWQEVAGVRPQRITRLGKDDNFWAMGDTGPCGPNTEIYWDFYPERGEDGDNPGTN
                     EARFFEIWNLVFMQFDQPGDGTMVPLEHPGVDTGMGLERMSTVMQSVPSSYETDLFTY
                     ILDKIQELTGHTHEERERDIISYRVVADHTRAATFLIGEGVLPGNNLREYVLRRIMRR
                     AMVHARRLGLTGPFMGEVADVIIESMGDVYPEIRQRRDFIPEAVRREEESFARTIITG
                     LTQFEQIINRLGLKPGEVFPGREMFELYGTHGLSKDIMKDEAVSRGLQIDEVGFEEAL
                     KEEQQRSKGSTILKGGERQNLDVYQQAAGNQSTTFLGYDYATLDGEAPRSKVLGIVTN
                     GEVVDLAGEGTDVEVVLDQTPFYAESGGQVGDKGFLVWEGGRMRVDDTQRPVGGVIVH
                     RGTVIEGTLRTGAEVEPEIPGGPRWSTMRNHTATHLLHKALRDVLGAHVHQKGSVVEP
                     GRLRFDFSHNAPMSQDELLTVERIVNEQVRADYPVQTAELPVKEAIAQGAMALFGEKY
                     GDIVRLVRIPGYESKELCGGTHVERTGQIGPFVVTYEGSIGQGVRRIEAVT"
BASE COUNT          720 a         1270 c         1086 g          634 t
ORIGIN      
        1 cctggccgta ggtgagttct acttgcacca gttgctgcaa caggtccttg atctgctgct
       61 tggcctgtaa cgccgcttcg ggcagcgtct ctcgcggggc gatgttgaag tagacctgga
      121 tgttgctgcc gtcgtagcgc acgtacgtca cctggtcgcc ctcaggggcc tgacgcaagg
      181 gggctgctat ctggctctcc tgggccgtca gcgtgggcac cgtggtgggc gtcgcgggcg
      241 gctgagtggg ctgtggcggg gtggtcggtg acgggttggg cgtagtggtc accactacca
      301 cctgcgccgt cggtgtaggc tcaggcggtc caggtgtgtc ggttaccacc actaccaccg
      361 tctgcaccac gggcgtagcg gtcggcggct ccggcgtgtc ggtcaccgcc actaccaccg
      421 tttgcaccac aggcgtagga gacggcggct ccggcgtagg cgttaccaca acctccacga
      481 cctgcgtcgt agcggtaggg gtgggggccg gcacgttcac attcacgtcc ctcggcctga
      541 ataagagcca cagtatcaag cccagcagca gcaacccagc gagtgcggcc cccacgataa
      601 gcggggtgtt gttcttcctg ggtggtggcg cggtgacgtt tacctgcccg cccccggctt
      661 gtcctccttg tgccatgttg tcgccctcct tggcttctct ttgtttcgat gtgctttcgt
      721 atggaatggg attgcatcac ttttaactgc gcaaccactc atccgttacg gctagcgtag
      781 ttggtcgggc tgctcgcgct actccataaa gcctcggaga agagaggaag gctgtcggta
      841 agggacggcc acgagccact acgccgtttc ccccttcaac cacctatcta tcttcgccgc
      901 cagccccagc aacaccaaca tcccacccgc gaccagcgcc caccagccca catcctccga
      961 gtcctgcgcc cccaggtaaa gggcgtaaca taccgacaca attagccccg caatcggccc
     1021 gtaataaagc caccgccgcc gcgacctggg aggatgaggt gccctcggcc agaactccag
     1081 caaaatcacc gtcagcacaa acagggacgc cagggtaagg ggcaccccca tgtactggat
     1141 aagcagcctc atccccagcc ccacggcacc cgcccccagc agaaagagcg ctcttaccac
     1201 aagctcgccg ctgcaccccc gcaggcagcc cgacgcctcg ccaaaggctc tcgtcactat
     1261 ggaaccaatg tctcgcggtc gttcagcact cacatcttcc ctcctcaatg cacgccaatc
     1321 aaacctcgta cctccgtacc cacccgtacg tctcacgcac gacacccctc tccaccccgc
     1381 gtcccttgac gctccccgcc gcccctcggt acaatggcct gtactatacc caccggccga
     1441 tacacagaaa tagccaccag cattactcat aacgtaaatt cgaatttaga atttacccaa
     1501 aggggcaccc atgacccgaa tagacgaccc aattcacaat ccgcaatccg aatttccaaa
     1561 tcccccctac atgtccagcc gggagattcg ggagcgcttc atccgctact tcgtggagaa
     1621 ggtcggctct acccacgtgc cctcctcctc catcgtgccc accaacgacc ctacgctcct
     1681 gttcaccacg gcgggcatgg tgcagttcaa ggacgtcttc ctcggcaacg agacccgccc
     1741 ctacaagcgc gccgtcacct cacagaaggt catgcgcgcc gggggcaagc acaacgacct
     1801 cgaaaacgtc ggcccctcca tccggcacca gaccttcttc gagatgctcg gtaacttctc
     1861 ctttggcgac tacttcaagc gcgaggccat cgcttttgcc tgggagttgc ttacctcgcc
     1921 ccaggagcag ggcggcttcg gcctcgaccc caatcgcctc tggcccacga tctacctgga
     1981 cgatgacgag tcgttcgtgc tgtggcagga ggtcgccggg gtgcggcccc agcgcatcac
     2041 gcggctcggc aaggacgaca acttctgggc tatgggcgat accggcccct gcggacccaa
     2101 caccgagatc tactgggact tctacccgga gcgcggcgag gacggcgata accccggcac
     2161 caacgaggcc cgcttcttcg agatctggaa cctcgtcttt atgcagttcg accagccggg
     2221 cgacggcacg atggtgcccc tcgaacaccc cggcgtcgat accggcatgg gcctcgagcg
     2281 catgtccacg gtgatgcaga gcgtcccttc ctcgtatgag accgacctgt tcacttacat
     2341 actcgataag atacaggagc tgaccggcca cacccacgag gagcgcgagc gggacatcat
     2401 ctcctaccgt gtggtggccg accacacccg cgccgctacc ttcctcatcg gggagggcgt
     2461 gctcccaggc aacaatctaa gggagtacgt gctgcgccgc attatgcgcc gcgctatggt
     2521 ccacgcccgc cgcctcggcc tcaccgggcc gttcatgggc gaggtcgctg acgtaatcat
     2581 cgagagcatg ggcgacgtat accccgaaat acgccagcgg cgagacttca tcccggaggc
     2641 cgtgcgccgc gaagaggaga gcttcgcccg caccatcatt accggcctca cccagttcga
     2701 gcagataatc aaccggctcg gcctcaagcc cggcgaggtc ttcccaggca gggagatgtt
     2761 cgagctttac ggcactcacg gcctgtcgaa ggatatcatg aaggacgagg ctgtgtcacg
     2821 gggcttgcaa atagatgaag tcggcttcga ggaggccctg aaagaggaac agcagcgcag
     2881 caagggcagt actatcctca agggaggcga gcgccagaac cttgacgttt accagcaggc
     2941 cgcgggcaac caatcgacca cgttcctggg ctacgactac gccaccctcg acggtgaggc
     3001 cccccgctct aaagtgctcg gcatcgtcac caatggcgag gtagtggacc tggccgggga
     3061 gggcaccgac gtagaggtgg tgctggacca gaccccgttc tacgccgaat cgggcgggca
     3121 ggtgggcgac aaaggcttcc tggtgtggga aggtgggcgc atgcgggtgg acgacaccca
     3181 gcgccccgtc ggcggcgtga tcgtgcacag gggcaccgta atagagggca cgctccgcac
     3241 cggggccgag gtcgagccgg agataccggg cggcccccgt tggtccacga tgcgcaacca
     3301 caccgccacc cacctgctcc acaaggccct gcgcgacgtg ctgggcgctc acgtccacca
     3361 gaaggggtcg gtggtcgagc cgggccgcct gcgcttcgac ttcagccaca acgcccccat
     3421 gagccaggac gagctactca cggtggagcg catcgtcaac gagcaggtgc gcgccgacta
     3481 cccggtgcag acggccgagc ttcccgtgaa agaggccata gcccagggcg cgatggccct
     3541 cttcggggag aagtacggcg atatagtgcg cctcgtgcgc atccccggct acgagagcaa
     3601 ggagctttgc ggaggcaccc acgtcgagcg caccggccag ataggaccct tcgtcgtcac
     3661 ctacgaaggc tccatcgggc agggcgtgcg ccgcatcgaa gcggtgacgg
//
LOCUS       DASRWZ010000088         7878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1172704, whole genome shotgun sequence.
ACCESSION   DASRWZ010000088 DASRWZ010000000
VERSION     DASRWZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172704"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..507
                     /locus_tag="VFH60_01230"
     CDS             244..507
                     /locus_tag="VFH60_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET6312430.1"
                     /translation="MNTNKQEALKRLAYIEGHLKGIRKMVEEDQYCVDVLKQTYAVQR
                     AIDKFESLLLHGHLKSCVVEGIHEGRDDEVLTELGELFDLAQR"
     gene            524..3973
                     /locus_tag="VFH60_01235"
     CDS             524..3973
                     /locus_tag="VFH60_01235"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET6312431.1"
                     /translation="MAIEQVEKQGTEGQVPSANGAPAGAKDIEITLPIGGMTCASCVR
                     RVEKSLAKVDGVRSAGVNLATERATVKYDPSSANVATLISAVERAGYSVPLQEEILPI
                     EGMTCASCVRRVEKSLAKLPGVESVAVNLATEQATVKYNPAMLGHAEMGKAVERAGYA
                     IRAEQRAIAAATSADADGAPVDTDAERRRRELNTVRAKFIVSLTSGLLIMAIMFLPLP
                     MSMEARNLVMFALATPVQFWAGWQFYKGALMAARYLSTNMNTLIAVGTSAAYLYSAFL
                     TFFPGAVRGAGLMPEAYYDTSTLIIALILMGRYFEVRAKGRTSDAIKKLMGLAPRTAR
                     VIRDGVEVDLPVEQVLVGDIVRVRPGDRVPVDGVVLEGESSVDESMLTGESLPVEKGP
                     GSHVIGATLNKSGSFTFRATKVGRDTALAQIVRLVEEAQGSKAPIQRLADQISSYFVP
                     VVLGVAALTFVLWYFVGPEPRFTFALVSMIAVLIIACPCALGLATPTAIMVGTGKGAE
                     YGVLIRGGEALEGAHKVNAIVLDKTGTLTRGKPSVTDVVASSGLPERELLRLVAAAER
                     GSEHPLGEAIVRHADELGLPHATVSGFKALAGHGIAATAEGRELLVGNARLMSERGIH
                     PDGLGDRAVELARDGKTPMYVALDGRHAGLIAVADTLKPESVEAVRELKALGLEVWML
                     TGDNEATARAIASQVGITNVMAEVLPEQKAAKVKELQAQGKVVAMVGDGINDAPALAQ
                     ADLGIAIGTGADVAMEASDITLVGGDVRGVVTAIALSRRTISTIRQNLFWAFIYNVVL
                     IPVAMGVLYPLFGLLLNPVMAAAAMGMSSVSVVSNSLRLRGFTAPKSAEEIVRPPLRR
                     RLADIGYLAGIGALALIVGALSMFLFKPSMGAMGGGMEEVNTAPVARVSLDTGGPVQP
                     GAPTTLRLTLTEASTGSPVGELAVEHDAPMHLIVVSRDLGHFAHIHPQSADKPGEWAI
                     EHTFPAEGDYVLYDEFAVVGNGEEVHRIDLKVGDASSPAAALSPNLGPQQSGEYTVTI
                     APQGEVRAGQTSAFLLNVSRDGSPVSDLQPYYGAASHVVVLDANASDFSHVHAVPGTE
                     APQMMDGGGMSEEAEMEELPAAFGPDLAFSHQFPKPGLYKVWSQFQHEGRVQTVAWVV
                     EVR"
     gene            4042..4311
                     /locus_tag="VFH60_01240"
     CDS             4042..4311
                     /locus_tag="VFH60_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HET6312432.1"
                     /translation="MSQITLNVPDISCAHCEKTILNALQGQPGVEAVQVSLPAKNVYL
                     TYDDQALSLDQVKEILDEEGYPVASVSEGAAPDTQRRGFIPLSSN"
     gene            4849..6003
                     /locus_tag="VFH60_01245"
     CDS             4849..6003
                     /locus_tag="VFH60_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6312433.1"
                     /translation="MSIRKYSRIPAFIGLLVGGLVLSACGGPAASTPVPVPTEGAPAT
                     STAGPSVQATPEQTAEGTATLTPGTSGGAQGKLVLYTTRSESLIKPVVDAFQQKNPGV
                     EVVLLTGSSGELSAKLLEEKANPQADVFINTDTIAMSSLASQGVFEPNPSTAVELVPE
                     EYRAEDATWVALTLRARVIMYNTNLVKAEDLPRSMFDLTDPKWKSQVGAADSTNGSVQ
                     AQIVAMRELVGEERTAEFIKGLVANETRFFGGHTDVRKAVGAGELKLGLVNHYYYQLS
                     RAEGAPVGVVYPDQAQGEMGLLVNSTNAGLVRGAPNLPAAHSFVEYLLTMEGQKIFAE
                     LNYEYPVREGVALAEGVQTLDQYRLAEIDLKTLFDELEPSRALMESAGLP"
     gene            6034..7704
                     /locus_tag="VFH60_01250"
     CDS             6034..7704
                     /locus_tag="VFH60_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET6312434.1"
                     /translation="MLATERRLLAIREWRRARGVAMPPLLLALALVSGLVAVTPLVYI
                     VVRAAGVSPDVWSRLWSGRVPGLLANTLMLVAFTAELTAGLGLGLAWLLDRTDLPGRN
                     FWRWVLALPLAVPAYVGALCYLIVLRRGGLLEQGLISLGWVEFGQVPLPSLYTLWAAT
                     LVIGLFTFPYVYLPVAAALRSTNWSLEEAARVGGQGAWGAFGRVVLPLLRPALLAGTL
                     LVCLYALSDFGTVALLQYRTFTVAIYNQFAGAVDRSGAAVLSVLLVLLSTPLLFLEAW
                     SHRRSRRYVNQASWKPRRLVPLGRWRWLAAGAVSLVGVLSLGLPLAVLGGLTVQGWLW
                     PTPLDVIWGVGGEGLWQYGLNSFLLAGLAASIAILLAFAPAYLASRYPNRFMLALLGV
                     GKSGYSLPGIIVGLSLVLLFSNWVPVLYGTVAVLVLAFGIRFLPQALASTEAAMKASS
                     PRLEQAARTLGRGPWQVFREITLPMIAPGLVAGWALVFLTSMKELPTAILLRPPGFDT
                     LPVRIWAAASESIYTQAAPPAFLLISLTVLVMALLFTRGKFGTDEVVL"
BASE COUNT         1403 a         2401 c         2667 g         1407 t
ORIGIN      
        1 caagggcagg cacgggggcc tgcccctaca tagcacccat tcgtgcctgg cgattacctg
       61 attaaccagt taatcttcat tagccgcagg gtgtccccac agtgccaagc ccgccccttc
      121 atgccgaaga tacgagtgcc cggggtcgcg attcagtggg gttgacaccg agggacgagc
      181 atgttatact agcgttgcaa accccctccg ggggggtagg gtataacaca gaggatgcat
      241 gctatgaaca caaacaaaca agaagccctg aagcgcctgg cctacatcga gggccacctc
      301 aaaggcatcc gcaagatggt cgaggaagac cagtactgcg tggatgtact caagcagacc
      361 tacgcggtgc agcgcgctat cgataagttc gagagcctgc tgctgcacgg ccacttgaag
      421 tcctgcgtgg tagagggcat acacgaggga cgcgacgacg aagtattgac cgagcttggc
      481 gagttattcg acctggcgca acgctaacgg aaagcatact acgatggcta tcgaacaagt
      541 agaaaaacaa gggacggaag gccaggtgcc ttctgctaat ggcgcgcctg cgggtgcgaa
      601 agacatagaa atcacgctgc cgataggcgg catgacgtgc gccagttgcg tgcgccgtgt
      661 ggagaagtcc ctcgcgaagg tcgacggggt gcgaagcgcg ggtgtgaacc tggcaaccga
      721 gcgggccacg gtgaagtacg acccctcgtc cgccaacgta gctaccctca tctccgcagt
      781 cgagcgggcg ggctattcgg tgccgctcca ggaagaaata ctgccgattg agggcatgac
      841 ctgcgccagt tgcgtgcgcc gcgtggagaa gtccctcgcc aagctgcccg gcgtggagag
      901 cgtggcggtg aacctggcga ccgagcaggc cacggtgaag tacaacccgg ccatgctggg
      961 ccatgccgag atgggcaagg ccgtcgaaag ggcggggtac gccatccgcg ccgagcagag
     1021 ggccatcgca gccgccactt cggcggatgc tgacggtgcc cccgtcgata cggacgccga
     1081 gcgcaggcgg cgcgagctaa acacggtccg cgccaagttc attgtgagcc tgacctccgg
     1141 cctgctcatc atggcgataa tgttcctgcc gctgcccatg tcgatggagg cgcgtaacct
     1201 cgtgatgttc gcgctggcga ccccggtgca gttctgggcg ggctggcagt tctacaaggg
     1261 cgcgcttatg gccgcccggt acctgtctac caacatgaac accctcatcg cggtgggcac
     1321 gtcggcggcg tacctgtaca gcgcgttcct gacgttcttc cccggcgcgg tacgtggggc
     1381 gggcctgatg cccgaagcct actacgacac ttccaccctg atcatcgccc tcatcctcat
     1441 gggccgctat ttcgaggtgc gcgctaaggg ccgcaccagc gacgctatca agaagctgat
     1501 gggtctcgcg ccccgcaccg cccgcgtgat tcgcgacgga gtagaggtgg acttgccggt
     1561 ggagcaggtg ctggtgggcg atatcgtgcg cgtgcggccc ggcgacaggg tgccggtgga
     1621 cggggtggtg ctggaaggcg aatcgtcggt ggacgagtcg atgctgacgg gcgagagcct
     1681 gccggtggag aagggtccag gctcccacgt gataggcgcg acgctgaaca agagcggctc
     1741 ctttaccttc cgcgctacga aggtcgggag ggataccgcc ctggcgcaga tagtgcggct
     1801 ggtggaggag gcgcaaggct cgaaggcccc aatacagcgg ctggcggacc agatttcgtc
     1861 ctactttgtg cccgtggtgc tgggcgtcgc ggccctgact ttcgtgctct ggtacttcgt
     1921 ggggccggag ccgcgcttca ccttcgccct cgtgtcgatg atagcggtgc tgatcatcgc
     1981 ctgcccctgc gccctcggcc tggcgacccc tacagccatc atggtgggca ccggcaaagg
     2041 tgccgagtat ggagtgctca tccggggcgg agaggccctt gagggcgctc acaaggtcaa
     2101 tgcaatcgtg ctggacaaga ccggcaccct caccaggggc aagccctccg tcaccgacgt
     2161 ggtggcctcc agcggcctgc cggagcggga gttgcttcgc ctggtggccg ccgccgaacg
     2221 cggttcggag cacccgctgg gtgaggccat cgtgcggcac gcggacgagc ttggcctgcc
     2281 ccacgccacg gtcagcgggt tcaaggcgct ggcgggacac ggtatagccg ccacagccga
     2341 gggacgcgag ctgctggtgg gcaacgcccg cctgatgtcg gagcgcggca tccacccgga
     2401 cggactgggt gatagggcgg tcgagctggc ccgcgacggc aagaccccta tgtacgtggc
     2461 gcttgacggg cggcatgcgg gcctaatcgc ggtggccgac acgctcaagc cggagtcggt
     2521 ggaagcggtg cgcgagctaa aggcgctggg cctggaagtg tggatgctga cgggcgacaa
     2581 cgaggccacc gcccgcgcca tcgcgtcgca ggtgggcatc acgaacgtca tggccgaggt
     2641 gctgccggag cagaaggccg ccaaggtgaa ggagttgcag gcgcagggca aggtcgtggc
     2701 gatggtgggc gacggtatca acgacgcccc ggccctggcg caagccgacc tgggtatagc
     2761 gataggcacc ggggctgacg tagctatgga ggcctcggac attacgctcg tgggcggcga
     2821 cgtgcgcggc gtggtcacgg ccatcgccct gtcgcgccgc accatctcca cgatacggca
     2881 gaacctgttc tgggccttca tctataacgt ggtgctgata ccggtcgcga tgggcgtgct
     2941 ctacccgctg ttcgggctgc tgttgaaccc cgtcatggcc gccgccgcga tgggcatgtc
     3001 gtcggtaagc gtggtgtcca actcgctcag gctgcgcggc ttcacggccc cgaagagtgc
     3061 cgaggagatc gtgcgcccgc ccctgcgccg ccgcctggcc gacatcggct acctggcggg
     3121 tataggtgcg cttgccctca tcgtgggcgc gctgtcgatg ttcctcttca agccgtctat
     3181 gggcgcgatg ggcgggggta tggaggaagt caacacggcc ccggtcgccc gcgtgagcct
     3241 ggataccggc ggccccgttc agcccggcgc gcccaccacg ctccggctga cgctcaccga
     3301 ggcttcgacc ggctctccgg tgggggaatt ggcggtggaa cacgacgcgc cgatgcacct
     3361 catcgtggtc agccgtgacc tcgggcactt cgcgcacata caccctcaat cggcggataa
     3421 gcccggagag tgggctatcg agcacacctt cccggcagag ggcgactacg tcctgtacga
     3481 cgagttcgcc gtggtaggca acggcgagga ggtccaccgt atcgacctga aagtaggcga
     3541 cgcgagtagc ccggcagccg ctctgtcgcc caacctcggc ccgcaacagt ccggcgaata
     3601 caccgtgaca atcgcgccgc agggcgaggt acgggcggga caaactagcg cgttcttgct
     3661 gaacgtatcg cgggatggca gccccgtcag cgacttgcag ccctactacg gcgctgccag
     3721 ccacgtggtt gtgctcgacg ccaacgcctc cgacttcagc cacgtgcacg cggtgcccgg
     3781 cacggaggcc ccgcaaatga tggacggtgg cggcatgtcg gaagaggcgg agatggagga
     3841 gctacccgcc gcgttcggcc ccgacctggc tttcagccac caattcccaa agccgggcct
     3901 ctacaaggtc tggtcgcagt tccagcacga aggccgggtg cagaccgtgg cctgggtggt
     3961 ggaagtgcgc taggttagtg ttcttgtctg ccttgcagac ggacgttgta tagaaaccaa
     4021 acacaaagga gcaaaaacaa aatgtctcag attacgttga acgtacccga tatatcctgc
     4081 gcccactgcg agaagacgat cctgaacgcc ttgcagggcc agcccggcgt cgaagcggtc
     4141 caggtgagcc tgcccgccaa gaacgtatac ctcacctacg acgaccaggc cctcagcctc
     4201 gaccaggtga aggagatcct cgacgaggaa ggctaccccg tggcctcagt ctccgaaggc
     4261 gcggcacccg acacgcagcg gcgggggttc atccctctta gcagcaacta gagagtacgg
     4321 aaccgccaag acgccaagga cgccaagagc gcgccaaggt attaaggctt ggagagtaca
     4381 aaaagaacca gggtacggca tcacgtaccc tggttctttt gcttagaact caaccataat
     4441 gccctggcgt gttcttggcg tcttggcggt tccgtactcc tgaatggctt tctactgaca
     4501 aacctgtcga aagtccttga caaaactttc aaattaagtt accttcattt acggtgctca
     4561 gaagaggcac gtcagccggg agatggctga aagccttgcc ggtactcgtg ggcatgcagc
     4621 gccacatttt cttacaaaga gccgggcgcg caagcctgca tagatgcaaa acggcgaaca
     4681 gggtggctgc cctgttcgcc tctgccgtag accgtccgtt ggaccaaacg ctctccagcc
     4741 tcctgatgat agcacaccgg aggctggtag gcaacacttg cttgctcatc gagggcgggc
     4801 aatgacacca aaatgcaacc gggaactgtc tcaggaggaa ctttaagaat gtcgatacgg
     4861 aagtactcgc gcataccggc gtttattggc ttgctcgtcg gtggcctggt gctgtcggcc
     4921 tgtggcggcc ctgctgcctc cacgccggtg cccgttccga ccgagggcgc gccggccacc
     4981 agcaccgccg ggccttcggt ccaggccacg ccggagcaga ccgcggaggg cactgccact
     5041 ctcacacccg gcacgtccgg tggggcgcag gggaagctgg tgttgtacac cacgcgctcg
     5101 gagtcgctca tcaagccggt ggtggatgcg ttccagcaga agaacccagg cgtggaagtg
     5161 gtgctgttga ccggcagttc gggcgaattg tccgcgaagc tgcttgagga gaaggccaac
     5221 ccgcaggctg acgtgttcat caacaccgac acgatagcca tgtccagcct ggcgagccag
     5281 ggcgtgttcg agccgaaccc ctccacagcg gtcgagctgg tgccggagga atacagggcc
     5341 gaggatgcta cctgggtcgc tttgacgctc agggcgcgcg tcatcatgta caacacaaac
     5401 ctcgtcaagg ccgaggacct gcccaggtct atgttcgacc tgaccgatcc taaatggaag
     5461 agccaggttg gcgccgccga tagcaccaac ggctccgtac aggcgcagat agtggcgatg
     5521 cgcgagctag tgggcgagga gagaacggca gagttcatca aggggcttgt cgccaacgaa
     5581 acgcggttct tcggtgggca caccgacgtg cgtaaggcgg tcggcgcggg cgagctgaag
     5641 ctggggttgg tgaaccacta ctattaccag ctctccaggg ctgagggcgc gccggtaggt
     5701 gtggtctacc ccgaccaggc acaaggcgag atgggcctgc tggtcaactc caccaacgcg
     5761 ggcctggtga ggggagcgcc caacctgccc gccgcgcact cgttcgtgga atacctgctc
     5821 acgatggaag ggcagaagat atttgccgaa ttgaactacg aatacccggt aagggaaggc
     5881 gtggcgcttg ccgagggcgt gcagaccctg gaccagtacc gcctcgcgga gatagacctc
     5941 aagacgctct ttgacgagct agagccgagc agagctttga tggagagcgc gggcctacca
     6001 taacaggacg agtgaggagt caggctaccg accgtgttgg cgaccgaacg cagactactg
     6061 gcaataagag agtggcgtag agcgcgtggg gtggcgatgc cgcccctgtt gctggctctt
     6121 gccctcgtgt cggggctggt cgcggttact cccctcgtct atatcgtcgt cagggcagcc
     6181 ggcgtatcgc cggacgtatg gagccgcctg tggagcgggc gggtgcccgg cctgctcgct
     6241 aacacgctga tgctggtcgc ctttaccgcc gagcttacgg ccgggctggg gctggggctg
     6301 gcctggctgc tggaccgcac cgacctgccc ggccgcaact tctggcgctg ggtgcttgcg
     6361 ttgccgctgg cggtgcccgc ctacgttggt gctctgtgct acctgatagt gctccgaagg
     6421 ggcggcttgc tggagcaggg gttgatatcc ctgggttggg tcgagttcgg ccaggtgccg
     6481 ctcccgtcgc tttacacact gtgggcggcc acgctggtta ttggcctgtt taccttccca
     6541 tacgtttacc tgcccgtagc cgctgccctg cgctctacca attggagcct ggaagaagcg
     6601 gcgcgcgtgg gcgggcaggg tgcctggggg gcgttcgggc gcgtcgtgct gcctttgctg
     6661 cggccggccc tgctggcggg tacgctgctg gtctgcctct acgccctctc cgacttcggc
     6721 acggtggcgc tgctccagta ccgcaccttc accgtggcca tctacaacca gtttgccggg
     6781 gcggtcgacc gcagcggcgc ggcggtgctg agcgtgttgc tggtgctgct ctcgacgccg
     6841 ctgctcttcc tcgaggcgtg gtcgcacagg cgcagcaggc gctacgtgaa ccaggcatcc
     6901 tggaagccgc gaaggctcgt gccgctgggc cgctggcgat ggttagctgc cggtgccgtg
     6961 tcgctggtgg gcgtactgtc gctggggttg ccgctggcgg tgctcggcgg gctgacggtg
     7021 cagggctggc tttggcccac cccgctagac gtcatatggg gcgtcggggg tgaggggctg
     7081 tggcaatatg gtcttaatag tttcctgtta gcgggactgg cggcaagcat agcgatattg
     7141 ctggcatttg cccctgccta cctggcctct cgctatccga atcgcttcat gctcgctctg
     7201 ttaggcgttg gcaagtcggg ctactcgctg ccgggcatca tagtcgggtt gagcctggtg
     7261 ctgctgttca gcaactgggt gccggtgctc tacgggaccg tggcggtgct cgtgctggcc
     7321 ttcggcatcc ggttcttgcc gcaggcgctg gcctccaccg aagcggcaat gaaagcgtct
     7381 tcacccaggc tggagcaggc cgcccgcacg ctgggccgcg gaccgtggca ggtgttccgc
     7441 gagataacgc tgccgatgat cgcgccgggg cttgtcgcgg gctgggcgtt ggtcttcctt
     7501 acctccatga aggagctacc aacggctatt ctgctgcggc cgcccggttt cgatacgctg
     7561 ccggtgcgca tctgggccgc cgcgagcgaa tcgatctaca cgcaggccgc gcctcccgcc
     7621 ttcctgctga tctcgctcac cgtattggtg atggcgttgc tgtttacgag gggcaaattt
     7681 ggtaccgatg aggttgtgct gtgagagaca ttagagactt taccgtagat acgggacacg
     7741 tcgtaatcga ggttggcaac gtgagcaaga gcttcgccgc gccgcaaagc ggggagcctc
     7801 gctggggccg ggggcggacc gccgtcctgg acccggcacg ctccgccgcg gtgcgggagg
     7861 tctccttgca ggtgcggc
//
LOCUS       DASRWZ010000089         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_7930,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000089 DASRWZ010000000
VERSION     DASRWZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7930"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VFH60_01255"
     CDS             complement(<1..1222)
                     /locus_tag="VFH60_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET6312435.1"
                     /translation="MNTALSLTKVRLLSFVLGAIALITVGGLTFSGAHASAITKAGSQ
                     PTAIASTATPAAAVGKSSRQAPAAPNDSCGLNSNYVVTASTGASLISGAAKVGSGCDE
                     CTHNITLPFPFRLYDQTFTSVVAGSNGTLGFPGNLNSVDYLCIPEAGYTYSIFPYLEN
                     MSTAGTTKGIYTLVTGTAPNRIFVVEWRTCTMIDVDMPCSGTNVFQVQLYEGQNKFDI
                     VYGQMTLGNAPAIGVQKDSTLFTGYACADSNISNGTRLSFIQSSCPTSTPTRTSTSTP
                     TNTPTRTATRTSTATNTPGGPTPTVTNTPVTAPNDVIISEFRLRGPNGANDEFVELYN
                     NTNGHIIVNSVDGSSGWALVAGDGATRFVVPNGTLIPARGHFLGVNSIAYSQAGYPAV
                     NGTTATGDATYNTDI"
     gene            1568..1693
                     /locus_tag="VFH60_01260"
     CDS             1568..1693
                     /locus_tag="VFH60_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312436.1"
                     /translation="MYFKPVGGLAMAVPILTIGITPMSDEHSFGAYLRQRRKLFG"
     gene            1715..>2990
                     /locus_tag="VFH60_01265"
     CDS             1715..>2990
                     /locus_tag="VFH60_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HET6312437.1"
                     /translation="MSGCSKALIRKIEAGERRPSRQIAELLMEGLEVPPEEQAEFMRL
                     ARGEGAVPGPAAMPPPPVVPAQLPLPRTTPVPAAAPAPPRAFPGSPAAADPNQAQFAT
                     ASLVAPATGAATPAPAPEAAVARPASGLPVQLTGLIGREDEVDSISGRLLDEDVRLLT
                     LLGPPGIGKTRLAIEVASELQGNFRDGVYFVGLATISDPGLVITTIAQTLGLREASGQ
                     TFAVALTNYLRGKQTLLVLDTFEQVIEAAPHVVDLLTACPGLKVLVTSREALHVRGER
                     QFPVLPLEMPAPGSRHPAGELLTYPAVQLFMERAQAVKPQFELTEENAAAVTAICVRL
                     DGMPLALELAAARIKLFPAHEIAARLDRSLPLLTGGARDLPARQQTLRGAIQWSYDLL
                     DEAEKDLFVRLGVFVGGCDLTGIEAICSGPEPE"
BASE COUNT          506 a          884 c         1007 g          593 t
ORIGIN      
        1 gtatgtcagt gttataggtg gcatcgccgg tggcggtggt accgttgacc gcggggtagc
       61 ctgcctggct gtaagcgatc gagttcacgc ccaggaagtg cccgcgtgcc ggtatgaggg
      121 tgccattggg cacgacgaac cgggtcgcac cgtcgccggc aaccagcgcc cagcccgagg
      181 agccgtctac ggagttgacg atgatatggc cgttcgtatt gttgtatagc tccacgaact
      241 cgtcgtttgc accgttaggc cctctcaagc gaaactcgct gatgatgaca tcgttgggag
      301 ccgttacggg ggtgttggtt acggtaggcg tggggccgcc gggagtattg gtggcggtac
      361 tggtgcgtgt agcggtcctg gtgggggtat ttgtcggtgt gctggtagac gtgcgcgtag
      421 gggtgctcgt ggggcaggag gactggatga aggagagcct ggtgccgttg ctgatgttgc
      481 tgtccgcgca ggcatatccg gtgaataggg tagagtcttt ctgcacgcca atcgcgggcg
      541 cgttgccgag cgtcatctgg ccgtacacga tgtcaaactt gttctggccc tcgtagagtt
      601 gcacctggaa gacgttggtg ccgctgcaag gcatgtcaac gtcaatcatg gtgcaggtgc
      661 gccattcgac aacgaatatg cggttggggg ccgtgcccgt caccagggta tagataccct
      721 tcgtggtgcc cgcggtgctc atgttctcca ggtaagggaa gatgctgtag gtatagcctg
      781 cttcgggtat gcacaggtag tccacgctgt tgaggttgcc tgggaagcca agcgtgccgt
      841 tgctgcctgc gaccacgctt gtgaaagtct ggtcgtacag ccggaagggg aacggcaggg
      901 tgatgttgtg cgtgcactcg tcgcagccgg aacctacctt ggccgccccg ctaatcaagg
      961 aagccccagt ggaggcggtg accacgtagt tgctgttcag gccgcacgag tcgttgggcg
     1021 cggcgggagc ctgccggctg ctcttgccga ccgcagccgc cggggtagcg gtcgaggcga
     1081 tggcggtggg ttggctgcct gcctttgtta tggcacttgc gtgcgcgcct gaaaacgtca
     1141 gcccgcccac cgtaatgagg gcgatagcgc ccaggacgaa tgaaagaagc cttaccttcg
     1201 tgagacttag cgcggtattc actttgtgtt ctctcctata gaccttttgt tgtgctggtt
     1261 tttatatggt gcgtttcggt tttgctgtgc ctgttaatgt tttgccgttt gtatatccgg
     1321 gttgtttata tatcttgccg gtaaggtgca tcgatgtatg tttgtgatgt ctgtattgta
     1381 gagaaaaggc tgctgccagg gaacaggttt gtaatacatg ttccaataca ggaactctga
     1441 caaaagtgat acaagagcag gacaaatcgc tacaggttcg cgtgtcaaac gtggtacaat
     1501 gtacatagaa gaaatacatc ggcaggttgg gcgttagagc gccgccagga cttcacgcgc
     1561 tcggccggtg tatttcaagc cagttggggg cctggctatg gccgtcccca tcctcacgat
     1621 tggcatcacc cctatgagcg acgaacattc ctttggcgcg tacctgaggc agcgccgcaa
     1681 attatttggc tagacccagg acgagcttgc cgagatgtcg ggctgctcca aggcgctgat
     1741 tcgcaagatc gaggccgggg agcgccgccc gtcgagacag atagccgagc tactcatgga
     1801 ggggctggaa gtgcccccgg aggagcaggc ggagttcatg cggctggcaa ggggcgaagg
     1861 ggctgtgccc ggtccggctg cgatgccccc gccccccgtg gtgcccgccc agttgcccct
     1921 gccccgcacc accccggtgc ccgcagccgc ccccgccccc ccgcgggcct tccccggctc
     1981 gcctgccgcc gcagacccca accaggccca gttcgccacg gcatcgcttg tcgcgcccgc
     2041 caccggcgcg gcaaccccgg caccagcgcc cgaagccgcc gtggcgcgcc ccgcctccgg
     2101 gctgccagtg caactcaccg gcttgatagg ccgggaggac gaggttgaca gcattagcgg
     2161 ccgcctgctg gacgaggacg tgcgcctgct gacgctgctg gggcctccgg gtatcggcaa
     2221 gacccgcctg gcgatagagg tggcatccga gttgcagggc aacttccgcg atggggtcta
     2281 cttcgtcggg ctggcgacca taagcgaccc cggccttgtg ataaccacga ttgcccagac
     2341 cctcggcctg cgcgaggcga gcgggcagac gttcgcggtg gcgctcacca actacctgcg
     2401 cggcaagcag accttgctgg tgctcgatac gttcgagcag gtgatagagg ccgcgccgca
     2461 cgtggtggac ctgctgaccg cctgccccgg cctcaaggtg ctggtcacga gccgggaggc
     2521 gttgcacgtg aggggcgagc gccagttccc cgtgctgcct ttggagatgc ccgcgcccgg
     2581 cagccggcac cccgccgggg agctactgac ctaccccgcc gtgcagcttt tcatggagcg
     2641 ggcgcaggcc gtcaagcccc agttcgagct aaccgaggag aacgcggcgg cagtgaccgc
     2701 tatctgcgtc aggctcgacg gtatgccgct ggcgttggag cttgcggcgg cccgcatcaa
     2761 gctcttcccc gcccacgaga tcgccgcccg cctcgaccgc agccttccac tgctcaccgg
     2821 cggggcgagg gacctgcccg cccgccagca gacccttcgc ggcgcgatcc agtggagcta
     2881 cgacctgcta gatgaggcag agaaggacct tttcgtcagg ctgggcgtgt tcgtgggcgg
     2941 ctgcgacctc accggcatag aggcgatttg cagcgggccg gagcctgaaa
//
LOCUS       DASRWZ010000090         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1310013, whole genome shotgun sequence.
ACCESSION   DASRWZ010000090 DASRWZ010000000
VERSION     DASRWZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310013"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VFH60_01270"
     CDS             complement(<1..715)
                     /locus_tag="VFH60_01270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6312438.1"
                     /translation="MPEILDDLSPLTLAWATDQNFIEFHKQMACLPGAEVHDEADLMW
                     FATPIKMGGFNGVVRASLRPEDVGQKVEEVAEMFRLRDTRWNWAVGPSSQPPDLGSRL
                     EEHGMSVGGGELAMAVDLHALNADVPRPEGLTVVRVRDAATLDAYMHAMVTGFGPPED
                     FSRQWRVVFDCLGLGEDAPFQHYLGLLDGEPVASSSVFYGAGVAGIYMVATVPQARGR
                     GIGSYITVAPLLDARDLGYR"
     gene            complement(848..2458)
                     /locus_tag="VFH60_01275"
     CDS             complement(848..2458)
                     /locus_tag="VFH60_01275"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET6312439.1"
                     /translation="MQAHQSYTVEAEGQVEMPDITGNSDTSGKVTRALPAHNRGLVAL
                     PPAQAFSARRQHVALVAARLVMDGFLLAAAFGLAYWLRYEIEIGRNVVADSSYQSFAF
                     FYPYMAAFVAIGLLSFHARSMYALPRGASWFDFMRVVFGASLVTVAALTLGALFFNPV
                     LPSRGLFLLLWVSTLAIFAVERFAFRMLRLWLWRRGINIRRAVVVGTGLAGQRIMKDI
                     VERTDLGYKLTGYVSDVKESPGGQNWRVPLRVRNGSMPQWLGTLKDVDRIICGRDVHE
                     VIVALPATHHAQILNIIDSCRACGVDFKLVPDLFEMRFNEVRIDALNGVPLIGVKDVA
                     LQGFNLFVKRVLDVTLAVGSLTVAAIPMLFIALAVKLNSPGPVLFQQKRVGKGGEEFT
                     CYKFRSMYEDAEQRLAEIAHLNEADGPIFKVKNDPRLTPVGRFLRRTSLDELPQLFNI
                     LKGEMSWVGPRPPLRREVDQYSDWHLKRLDVTPGLTGLWQVSGRSDLSFEEMVKLDIY
                     YAENWSLAMDATIILKTIPAVVKRQGAY"
BASE COUNT          488 a          841 c          831 g          446 t
ORIGIN      
        1 cgcggtagcc gaggtcgcgg gcgtcgagga ggggtgccac cgtgatgtac gagcctatgc
       61 cccggcccct cgcctggggc acggtggcta ccatgtagat gccggccacc cccgcgccgt
      121 agaagaccga ggagcttgcc acaggctcgc cgtcgagcag gccgagatag tgctggaagg
      181 gcgcgtcctc ccccaggccc aggcagtcga acaccacgcg ccactggcgg ctgaagtcct
      241 ccggcggccc gaagcccgtg accatcgcgt gcatgtaggc gtcgagcgtg gcggcgtcgc
      301 gcacccgcac caccgtaagc ccctccggcc tcggcacatc ggcgttgagc gcgtggaggt
      361 cgacggccat cgccagctcg cccccgccta ccgacatgcc gtgctcttcc aggcgcgagc
      421 cgaggtccgg cggctggctg ctggggccga cagcccagtt ccacctggta tcgcgcaggc
      481 ggaacatctc agcgacctcc tccaccttct gccccacgtc ctccggccta aggctggcgc
      541 gcaccacccc gttgaaccca cccatcttga taggcgtggc gaaccacatc aggtccgcct
      601 cgtcgtgcac ctccgcgccc ggcaggcagg ccatctgctt gtggaattcg ataaagttct
      661 ggtccgtagc ccaggcaagc gtcagcggcg acaggtcatc taggatttca ggcaataggg
      721 ttccttcatg gatgatggga agtaacgcgt aacgcgtaat gagtaaagga gcctggcgta
      781 acaaaacggc tcacatggta catagcctca tgagccgcat tacgtattag gcgttacgtg
      841 ttacttccta atacgctccc tgccgtttga ccaccgcggg gatcgtcttg aggatgatcg
      901 tcgcgtccat cgcgagcgac cagttttcgg cgtagtaaat gtcgagcttg accatctcct
      961 cgaacgacag gtcggagcgg ccgctcacct gccacagccc cgtgaggccg ggggtcacgt
     1021 cgaggcgctt gaggtgccag tcgctgtact ggtccacctc gcggcgcagg ggcgggcggg
     1081 ggccaaccca gctcatctcg cccttcagga tgttgaatag ctggggcaat tcgtccagcg
     1141 aagtgcggcg caggaacctg cctaccgggg tgaggcgggg gtcgttcttg accttgaaaa
     1201 tcggcccgtc cgcctcgttg aggtgcgcta tctcggccag gcgctgctcg gcgtcctcgt
     1261 acattgagcg gaacttgtag caggtgaact cctcgccgcc cttgcccacg cgcttctgct
     1321 ggaacagcac ggggccgggg gagttgagct tgacggccag cgcgataaag agcatcggga
     1381 tggccgccac ggttagcgag ccgaccgcca gcgttacatc cagcacccgc ttgacgaaca
     1441 ggttgaagcc ttgcagcgcc acgtccttca cgccgatgag gggcacgccg ttcagagcgt
     1501 cgatgcgcac ctcgttgaag cgcatctcga acaggtcggg caccagcttg aagtccaccc
     1561 cgcaggcgcg gcagctatcg atgatgttga ggatttgggc gtggtgggtg gcgggcaggg
     1621 ctacgatcac ctcgtgcacg tcgcgcccgc agatgatgcg gtccacgtcc ttgagagtgc
     1681 cgagccactg gggcatggag ccgttacgca cccgcagagg cacccgccag ttttgcccgc
     1741 cggggctttc cttcacgtcc gacacatagc cggtgagctt atagccgagg tctgtgcgct
     1801 ccacgatgtc cttcatgatg cgctgcccgg ccagccccgt gccgaccacc accgccctgc
     1861 gtatgttgat gccccgccgc cacagccaca ggcgaagcat gcggaaggcg aaccgctcta
     1921 cggcgaaaat cgccagggtg gatacccaca gcaggaggaa aagccctcgg ctggggagca
     1981 ccgggttgaa gaagagggca cccagcgtca gcgccgccac ggtgaccagg gaagcgccga
     2041 acaccacgcg catgaagtcg aaccaggaag cgccacgagg gagggcgtac atgctacgag
     2101 cgtggaagga gagcagcccg attgccacga aggccgccat gtaggggtag aagaaggcga
     2161 aggactggta cgagctatcg gccaccacgt tgcggccaat ctcgatctcg tagcgcagcc
     2221 agtaagccag gccgaaagcc gccgccagca gaaagccgtc catcacgagc cgggccgcca
     2281 cgagagctac gtgctgacgg cgggccgaaa acgcctgtgc cgggggcaaa gccaccaggc
     2341 ccctgttatg ggcgggtagg gcgcgcgtaa ccttgccact cgtgtccgaa ttgcctgtga
     2401 tgtcgggcat ttccacctgc ccctcggctt ctaccgtgta actctggtga gcttgcataa
     2461 tcttgaaagt gccgagttcc gagttccgag taatacgact tatcccagtg aatacgtgag
     2521 tgcggacggg ttggactgct gagactctct cccgcccttg tcattctgag cgtagcgaag
     2581 aatctcgtcc ctcaccggga tgttcc
//
LOCUS       DASRWZ010000091         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_898911, whole genome shotgun sequence.
ACCESSION   DASRWZ010000091 DASRWZ010000000
VERSION     DASRWZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898911"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFH60_01280"
     CDS             complement(<1..566)
                     /locus_tag="VFH60_01280"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HET6312440.1"
                     /translation="MDNVEQLKTPSRTFKTLQYLTLVSRLVLGVIFALAGLAKLGLPF
                     HMAESIRAYEMGLPPFLVDFMANVLPTIELGVGIWLLLGLFTRFSATITAALMVVFTI
                     AITQAWMRGLDINCGCFGGPVGDDPSVTPAARAIIGAMGPVGTFLTNEKADLMTIGRE
                     RILLLMSIHLILVPSVFSLDQLRARARR"
     gene            complement(647..1465)
                     /locus_tag="VFH60_01285"
     CDS             complement(647..1465)
                     /locus_tag="VFH60_01285"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6312441.1"
                     /translation="MNKEANPRPTGTQNRPTGAGTSTKQSVTNRPAGVPPRPPARNGS
                     SAGTQGRSGSGAPRRQPPKQQSIGLKPLDIALLVAGVSVVVIVAFFVLSAQTGQPTMA
                     PGAGTSNQQNQAAQEPPKLAEGTVAPDFTLPGVDGNTYKLSDYRGKVVLLEFMAPWCP
                     HCQADAPMFNQVAQSFQGQDVQVFAISATPDNKDRNGPISMADMTWFRDTYSVNYPML
                     FDQPLAAAKAYQIYYYPTVYIVDKEGKIAKFIISETNNPITVERLTNEINNVLQ"
     gene            complement(1600..2817)
                     /locus_tag="VFH60_01290"
     CDS             complement(1600..2817)
                     /locus_tag="VFH60_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET6312442.1"
                     /translation="MAKLTVRDADVAGKRVLVRVDFNVPLDAGGQITDDTRIEASLPT
                     IRYLLENGAAVILMSHLGRPKGGPDPKLSLAPTAARLQELLGSGVTVRFVGDCTGAEV
                     KHAARDLQPGEVLVLENVRFHAEEEANNADFAGELAGIADIYVNDAFGSAHRAHASTE
                     GIARHLPAYSGLLMEREISIMGKALEDPERPFVAILGGAKVSDKMGVIGNLLSKVDVL
                     VIGGGMANTFLKATGVPIGQSLVENDRVRDAEALVGRANLQNVRLMLPRDAVIAGEMS
                     ATAQATRVDLHDLPPDIDPEGWRILDIGPESAAEYGAVIREAKTIVWNGPMGVFEIEA
                     FAAGTLAVAQAVCDATREGATSIVGGGDSVAAIEKMGLADCITHVSTGGGASLEFLEG
                     QTLPGVAALLDAG"
     gene            complement(2944..>3530)
                     /locus_tag="VFH60_01295"
     CDS             complement(2944..>3530)
                     /locus_tag="VFH60_01295"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET6312443.1"
                     /translation="REHTIVSNASCTTNCLAPVAKVILEKFGIVKGLVTTVHSYTNDQ
                     VILDYPHKDLRRARAAAMNIIPTTTGAAKAVSLVIPELKGKFDGFALRVPTPTVSIID
                     FVAQIERSATAQEINGALIEASENELVDILDYTEEPLVSMDFKGDPHSSIVDGLSTMV
                     IGENLVKVIAWYDNEWGYACRVADLTQFMAEKGT"
BASE COUNT          608 a         1182 c         1026 g          714 t
ORIGIN      
        1 tgccgccgcg ctcgcgcccg tagctggtcg agggagaaaa cgctcggcac gagtatcagg
       61 tgaattgaca taagcagcaa gatacgctcg cgcccaatcg tcatcaggtc ggccttctcg
      121 ttcgtcagga acgtgcccac cggccccatg gccccgatta tggcccgcgc agccggggta
      181 acgctggggt cgtcgcccac cggcccaccg aagcacccgc agttgatgtc cagcccccgc
      241 atccacgcct gcgtgatcgc tatcgtgaac accaccatga gcgccgccgt tatcgtagct
      301 gagaagcgtg tgaacagccc cagcagcagc cagatgccca cccccagctc gattgtgggc
      361 agcacgttag ccatgaaatc cactaggaaa ggcggcagcc ccatctcgta agccctgatg
      421 ctctcggcca tgtggaaagg cagccccagc ttggccagcc ccgccagcgc gaagatcacc
      481 cccagcacca gccgcgatac cagcgtcaag tactgtaggg tcttgaacgt cctgcttggg
      541 gtcttgagtt gctccacgtt gtccatccgc tttaccttcc tgatataaag agtgttgagt
      601 tagccgtggt aggccgactc aacactcaaa actcataact cagaacttac tgaaggacgt
      661 tgttgatttc gttcgttaga cgctcgaccg ttatggggtt gttggtctcg ctgattatga
      721 acttggcgat cttgccttcc ttgtccacta tgtagaccgt ggggtaatag tagatctggt
      781 aggctttggc ggcggcgagc ggctggtcga atagcatcgg gtagttcacg ctatacgtgt
      841 cgcggaacca ggtcatgtcg gccatgctga tggggccgtt gcggtccttg ttgtcaggtg
      901 tggcgcttat cgcaaacacc tgcacgtcct gcccctggaa gctctgcgcg acctggttga
      961 acatgggcgc gtcggcctgg cagtgcgggc accacggggc catgaactca agcagcacca
     1021 ccttgcccct gtagtcgctc agcttgtagg tgttgccatc aacgccgggc aaggtaaaat
     1081 cgggagccac ggtgccctca gccagcttgg gcggctcctg ggcggcctgg ttttgctggt
     1141 tgctcgtacc tgcaccaggg gccatggtag gctgcccggt ctgggcgctc agcacgaaga
     1201 acgcgacgat taccaccacc gatacgcccg ccaccagcag tgctatgtcg agcggcttga
     1261 ggccgataga ctgctgcttg ggcggctgtc gcctgggagc gccggacccg ctacggccct
     1321 gcgtacctgc agaggagccg ttgcgtgccg ggggcctggg cggaaccccc gccgggcgat
     1381 tagtcacact ctgcttggtg gatgtgcccg ctcctgtggg ccggttctgt gtgcctgtgg
     1441 gacgtgggtt agcttcttta ttcatgaatg atttatgcct ctcctatgtt gactcgacta
     1501 taagttttag gggcgtgcgt acacgccccc gtaattataa ccgatttggg ttacacgtcg
     1561 gaaattcgaa tttggaaatc cgaaattcga attttcggcc taccccgcgt cgagcaaggc
     1621 cgccacaccc ggcaacgtct gcccttccag gaactcgagc gacgccccgc ccccggtgga
     1681 tacgtgcgtg atgcaatcgg ccagccccat cttctcgata gccgccaccg agtcgccccc
     1741 gcccacgatg ctggtagcgc cctcgcgggt ggcgtcgcac accgcctgag ccaccgccag
     1801 cgtgcccgcc gcgaacgcct ctatctcgaa cacgcccatc ggcccgttcc acacgatggt
     1861 cttggcctcg cgtatcacgg ccccgtactc tgccgccgac tccggcccta tgtcgaggat
     1921 gcgccagcct tccgggtcta tatccggcgg caggtcgtgc aggtcaaccc tggtggcctg
     1981 ggccgtggcg ctcatctcgc ccgcgattac cgcgtccctg ggcagcatca ggcgcacgtt
     2041 ttgcaggttg gcccgcccca ccagcgcctc cgcgtccctc acccggtcgt tctccaccag
     2101 agactgcccg atggggacgc ctgtcgcctt gaggaaggtg ttagccatgc ccccgcctat
     2161 aacgagcaca tccaccttgc tcagcagatt gcctatcacc cccatcttgt ccgacacctt
     2221 ggccccgccc agtatcgcca cgaagggccg ctccgggtcc tccagcgcct tgcccatgat
     2281 gcttatctcg cgttccatca gcagccccga atatgcaggt aaatgccggg cgataccctc
     2341 cgtcgaggcg tgcgcgcggt gggccgatcc aaaggcgtca ttgacataaa tatcggctat
     2401 gccggcaagc tcccccgcga agtcggcgtt gttggcctcc tcttcggcgt ggaagcgcac
     2461 gttctcaagc accagcacct cgcccggttg caggtcccgc gccgcgtgct tcacctcggc
     2521 gcccgtgcag tcgcccacga acctcaccgt gacgccgcta cccagcagct cttgcaggcg
     2581 ggcagccgtg ggcgcgaggc tcaacttcgg gtccggcccg cccttcggcc tccccaggtg
     2641 cgacatgaga atcaccgctg ccccgttctc cagcaggtac cgtatcgtgg gcagcgaggc
     2701 ctcgatgcgc gtgtcgtccg tgatctgccc gcccgcgtcc agcggcacgt tgaagtccac
     2761 ccgaaccagc accctcttac ccgcgacatc cgcatcccgc accgtgagtt ttgccacaac
     2821 gcttacctcc ggggtgttga atgtcacgtg gtgccctgtg ggggtgccca gtggcattag
     2881 ccacaggaca cccccacagg actatcctca actcaaaact caacactcat aactcaaaac
     2941 tacttacgtc cccttctccg ccatgaactg cgtcaggtcg gccacgcggc aggcgtagcc
     3001 ccactcgttg tcgtaccagg cgattacctt caccaggttc tcgcctatga ccatcgtgct
     3061 cagcccgtcc acaatcgagg agtgcgggtc gcccttgaag tccatgctga cgagcggctc
     3121 ctcggtgtag tcgaggatgt ccaccagctc gttctcgctc gcctctatca gcgcgccgtt
     3181 gatctcctgg gcggtagccg agcgctctat ctgggccacg aagtcgatga tcgacacggt
     3241 tggggtgggt acgcgcaacg cgaagccgtc gaacttaccc ttcagctccg gtatcaccag
     3301 cgacaccgcc ttggcagccc cggtggtcgt cgggatgatg ttcatcgcgg cggcgcgggc
     3361 gcggcgtagg tccttgtgcg ggtagtcgag gattacctgg tcgttggtgt acgagtgcac
     3421 cgtggtcacc aggcccttga cgatgccgaa cttctccagt atcaccttgg ccacgggcgc
     3481 gagacagttg gtggtgcacg atgcattcga gacgatggtg tgctctcgcg
//
LOCUS       DASRWZ010000092         6311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_282289, whole genome shotgun sequence.
ACCESSION   DASRWZ010000092 DASRWZ010000000
VERSION     DASRWZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282289"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..863
                     /locus_tag="VFH60_01300"
     CDS             51..863
                     /locus_tag="VFH60_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312444.1"
                     /translation="MADTSPTTWKALTLVADGRTTLFEVAAAEGAPISRDMSYRSDDP
                     ELRLAASVDGQAVREYLLPTRQLELAGEPPYYFHMTLHVLGTLGVMLNCSITHSDEPL
                     STVDPSARYGSQGVRFQPWYLPGSQYSNESMAGRGLFTRGLHVSGIITPGNVCSCCVC
                     DRCARSFVLRQIHAGFSDCQYFYCERGLHTLLVYEPIEGMPFQLQGPDHGMAFQEQLP
                     PCQACNTHFRYYNPLRCPHCNAPYVDFATYPEMRPNEYYAYFHLGTELQKLG"
     gene            complement(994..2247)
                     /locus_tag="VFH60_01305"
     CDS             complement(994..2247)
                     /locus_tag="VFH60_01305"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6312445.1"
                     /translation="MGLKLTGLWRHPDFVKLWAAHTVSALGTGITGQALPLTALLVLG
                     ASAGEMGLLAAITQVPVVVILFAGVWVDRLRRRPILIAADVGRAALLVSIPIAAVAGA
                     LSLGQLFVVAALVESLSLFFSVAYRSYLPSLVQRERLVEGNSKLSAGGSVAEISGPGL
                     GGVLVQWLSAPLAILFDAASFLLSALFLGAIRTPEPPPKPAEERQSVRSEITEGLRVV
                     LSNPVLRALAACSGTFTFFGNFFALYPLYVVRDLGMPPALVGLFVGAGGIGALVGAML
                     VGPVTRRFGVGPTLISSLAFAGIVGSSILFVDGPLPLNVAVFFAAQLVGDIAICIYLI
                     NDMSLRQAMVPDRLLGRATASMGFLVGALGPVGALLGGLLGELIGARHTLAIAVIAIT
                     LAPLWLFFSPARALREPPTLPEATT"
     gene            3171..5747
                     /locus_tag="VFH60_01310"
     CDS             3171..5747
                     /locus_tag="VFH60_01310"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6312446.1"
                     /translation="MAEGVGFGGWLRQRRKEAGLTSKELAQQVGCSVATIEKMEAGIR
                     RPSRQIAELVGEFFGVGSEELPAFVEFARSEHAGEQPMPADEAASRAPWRILHNLRAL
                     HNLRSRPNNLPAQRTAFVGREREVAALSSGLRRPEVRLFTLTGPPGSGKTRLALHVAA
                     ELLDEFQDGCFFVSLAPIVDRESVAPAISLALRVPQVAGSSAVESLLEYMRGRQLLLL
                     LDNCEQVLEAAPLVADLLTWAPGLKIIATSRQAFRLYGEREIELPPMSLPDPQVPLAP
                     DELMRYEAIRLFVDRAQAAKYDFALTTDNAASVATTCRRLDGLPLGIELAASHVKSLS
                     PEEIATRLESRLELLDGGPIDLPPRHQTVRNAIEWSYDLLDEAERRLFRRLAVFKGGF
                     TLAAVEEVAGLVAGQVRVGPGEEHTGTSATLESLASKSLVQVGVGPDGAPRYWLLATI
                     AEYAWEQLASSGEAEELQGRHMAYYLKLVEQAEPQLRGPEQTLWLDLLDAEYDNLRAA
                     LRCCAEGRDVYAGLCLSGGLWRFWLVRGYLAEGRGQLAAALAVAQDSPGARLSRRHDP
                     RGYVKARAKALNGAGVLAGVQGDYETATAFFEEGLALYRRLDDRPNVAGMLSNLGNIA
                     KSQGDYSRARALQEESLAIRRELNDRQGMAASLANLGNVAHAQGDFTSARSLHEESLG
                     IASQLGDSWGVATTLNNLGTVAHEQGDLEAARAFFEESLAMRQALGDRRGIAKSLHNI
                     GSVIKDLKAYADAAARCKESLAIYSELGDRTGAAVCLESLAGIASLQGQARRAALLGG
                     AAEALREALGNPIPTAQQAEHEARVAAARRQLGEVPFAQAWKKGRALALEEAIALAQG
                     QA"
     gene            complement(5825..>6311)
                     /locus_tag="VFH60_01315"
     CDS             complement(5825..>6311)
                     /locus_tag="VFH60_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009314121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312447.1"
                     /translation="PSTAADSPAFDPLRYWSGPSWPVTNWLVIRALRERHDEARRLGE
                     VLRRSTLAVIEEGAGAEQARRAAALCMEANSAGEEFTTPSRRQYQHGWLWDSAIAAAC
                     WPLVPTKPGPAEAVAVEVGTPGFWEYYHPHTGEPLGAPRMTWTAALYLELLHMGDTPP
                     S"
BASE COUNT         1161 a         1904 c         2186 g         1060 t
ORIGIN      
        1 ctgcccctac ggtgcttttg caatacgatc ttaggaagtg tgaggtgtga atggcagata
       61 catcaccaac aacctggaaa gcgctgaccc tggtggcgga cggcagaact accctgttcg
      121 aagtagcagc cgcagagggc gcgcccattt caagggatat gagctacagg agcgacgacc
      181 ctgaactgag actagcagca agcgtagacg gccaggcggt gcgcgagtat ctcctgccca
      241 ccaggcaact agagcttgcc ggagagcctc cctactactt ccacatgacg ttgcacgtgc
      301 tcggcacgct gggtgtcatg ctcaactgct ccataaccca cagtgatgag ccgctatcca
      361 ctgttgaccc ctccgccaga tacggctcgc agggcgtgag gttccaaccc tggtacctgc
      421 ccggctcaca atacagcaac gagtcgatgg ccgggcgagg gctgttcacg agaggcctgc
      481 acgtaagcgg catcataacg ccgggtaacg tctgctcctg ctgcgtctgc gaccgttgcg
      541 ccaggtcgtt cgtgctgcgc cagattcacg cgggcttcag cgactgccag tacttctact
      601 gcgagcgcgg cctgcacacc ctcctggtct acgaaccaat agagggtatg cccttccagc
      661 tacagggtcc agatcacggc atggctttcc aggagcaact gccgccctgt caggcctgca
      721 acacccactt tcgctactac aaccccctgc gctgtcccca ctgcaacgcg ccctatgtag
      781 acttcgctac ctaccctgaa atgcgcccca atgaatacta cgcctacttc cacctcggca
      841 ccgagctaca gaagttgggc tgagatggac gatggacgat aggaataatg aggactgagg
      901 actgaggact gagtagctgt cagaacgcgt gccaacaaca atcctaatcc ttggcgcgct
      961 cttggcgtct tggcggttcc gccttttcaa gtttcacgtt gtcgcttcgg ggagggtggg
     1021 tggttcgcgg agggcgcggg cgggggagaa gaagagccag agcggggcga gggtgatggc
     1081 gatgactgct atcgccagcg tgtgcctcgc gcctatcaac tcgcccagaa ggccgccgag
     1141 gagcgcgccg acaggcccca gcgcgcccac cagaaagccc atgctggcgg tggcgcgtcc
     1201 cagcaggcgg tcgggcacca tcgcctggcg caggctcatg tcgtttatca ggtagatgca
     1261 tatggcaatg tcgcccacga gctgagcggc gaagaagacc gcgacgttca ggggcagcgg
     1321 cccgtccacg aacaggatgg aggagcctac gatgccagcg aaggcgagcg agctgatgag
     1381 ggttggcccc acgccgaagc gccgtgtgac aggccctacc agcatggcac ccactaacgc
     1441 cccgatgccc ccggccccta cgaagaggcc caccagggcg gggggcatgc ccaggtcgcg
     1501 cacgacgtac aatggataga gggcgaagaa gttgccgaag aaggtgaacg tgccggagca
     1561 ggcggcgaga gcgcgcagca ccgggttgct cagcaccacg cgcagtccct cggttatctc
     1621 gctacgcacg ctctgccgtt cctccgcggg tttgggtggt ggttcgggcg tgcgtatcgc
     1681 gcccaggaag agggccgaga ggaggaacga ggccgcgtcg aacaggatgg cgaggggggc
     1741 ggagagccac tgcaccagca ctccaccgag cccggggccg ctgatctcgg ccaccgagcc
     1801 gcccgcgctg agcttgctgt tgccctccac cagccgctcg cgttgcacca ggctgggcag
     1861 gtaggagcgg taggccacgc tgaagaagag ggagagcgat tccaccagcg cggccacgac
     1921 gaatagctgc cccaggctga gcgcgcccgc gacggcagcg attgggatgg agacgagcag
     1981 cgcggcccgc cctacatccg ccgctatcag gatggggcgg cggcgcaggc ggtcaaccca
     2041 taccccggcg aacaggatga ctaccaccgg cacctgcgtg atagccgcga gcaggcccat
     2101 ctcgccagcc gaggccccaa gcacgagcaa tgcggtgagg ggcagcgcct ggcccgtgat
     2161 gccggtgcct agcgccgaca ccgtgtgcgc ggcccacaac tttacgaagt ccgggtgcct
     2221 ccacagcccc gtcagcttca gtcccataac agccagtctc ccatgctccg tagcggcgtg
     2281 ggtatcatac gtggctggcc gctgtactac atcgggcgtg gggcgtatct tgtcagggct
     2341 aagccgcggt tgaaagcgct gggcaggacg cggatagcag cacccaaatc ccccttgtca
     2401 tcctgagtga tagacgttgc aagtaagtac ccaataagag gaccaagaga ggcattgtca
     2461 tcctgagcgt agcgaaggat caggtggcag ccggtctctg tggcaatgcg tgaggctggc
     2521 ggcaagccaa cctgggtagc ccaatacagc ttcttcccta cccaactgat ccttcgctac
     2581 gctcaggatg acaaggggct gtttgggtgc tgtagttgct ggcttgacga ctttggcaag
     2641 accgggagac acgcggaaat gaacaaaggg aaagcccaca tgccctggac tttccctccg
     2701 ttcaaggaga taggtagtat gctgccttat gcttctaaga cagcactatg ttggctactg
     2761 tgaagtgccc acctcgcaag gctttcgcca ggtcttgctt ttccttgctc cttcaaacat
     2821 tactgactaa gcctccacca tttgcacgtt tgctgtcgaa taatgatgtg gttggggatt
     2881 gcggccccat aatttcatac tgaggagtgt tacgcggtga gtactattta gtgatgagcg
     2941 tttgcggtta gagggcgagg actggcccct tgcggtacag agacgggagg aattgctcta
     3001 atcgcaggtg tgctgcccgg ttatcgggcg cggtttaaca ataccaggcc aggggaggcg
     3061 tgcgcgaggg gaattcgcgg acaattaccg gacaaaatca ggacagtcac aggcgggagc
     3121 gcgcgtttgg ccgggggcgc tctatactgg tagtacaggt tggaggcatc atggccgagg
     3181 gggtcgggtt cggagggtgg ctgcggcagc ggcggaaaga ggcagggctt acgagcaagg
     3241 agcttgcaca gcaggtcggc tgctcggtcg ccacaatcga gaagatggag gcaggcatcc
     3301 gccgcccttc caggcagatc gcagagttgg ttggggagtt ctttggtgtc gggagcgagg
     3361 agcttccggc attcgtggag ttcgcgcgct cggagcatgc gggcgagcag cccatgcccg
     3421 ctgacgaggc ggcgagccgc gccccctggc gtatactgca caacctgcgg gcgctgcaca
     3481 acctccgctc gcgccccaat aacctgcccg cccagcgaac ggcgttcgtg ggccgcgagc
     3541 gggaagtggc cgcgctctcc tccgggctgc ggcggcccga ggtgcgcctc ttcaccctca
     3601 ccggcccgcc gggcagcggc aagacccggc tggccctaca cgttgccgcc gaattgctcg
     3661 acgagttcca ggatgggtgc ttcttcgtgt ccctcgcgcc tatcgtagac cgcgaatcgg
     3721 tggcccctgc tatctcgctc gcgttgagag tgccgcaggt ggcgggtagc tcggcggtag
     3781 agagcctgct ggagtacatg cgtggcaggc agttgctctt gctgctggac aactgcgagc
     3841 aggtgctcga agctgctcca ctggtggccg acctgctcac ctgggcgccc ggcctgaaga
     3901 taatagccac cagcaggcaa gcgttcaggc tgtacggcga gcgcgagata gaattgcccc
     3961 caatgtcgtt gcccgacccg caggtacccc tcgcccccga cgaactgatg cgctacgagg
     4021 cgataaggct gttcgtggac cgcgcgcagg ccgccaaata cgacttcgcg ctcaccacgg
     4081 acaacgccgc gtccgtggct accacctgcc ggcggctcga cgggctgcca ctcggcatag
     4141 agcttgccgc ctcccacgtg aagtccctgt cgccggaaga gatagccacg aggctggaga
     4201 gcaggctgga attgttggac ggtggcccta tcgacttgcc gccccggcac cagacggtga
     4261 gaaacgccat agagtggagc tacgatctgc tggatgaggc cgaacgcagg ctcttccgcc
     4321 gcctggctgt gttcaagggc ggctttaccc tggcggcagt cgaggaggtc gcgggcttgg
     4381 tggcggggca ggtccgggtg gggccgggcg aggagcacac cgggacgtcg gccactctcg
     4441 aatcgctggc gagcaagagc ctcgtgcaag tgggggttgg acccgacggc gcgccccgat
     4501 actggctgct ggccacgata gcagagtacg cctgggagca gttggctagc agcggcgagg
     4561 ctgaagagct acagggcagg cacatggcct attacctgaa gctggtcgag caggccgagc
     4621 ctcaacttcg cggcccggag cagactttgt ggctcgacct gctggacgcc gagtacgaca
     4681 atctgcgcgc ggccctgcgg tgttgcgcag agggtaggga tgtgtacgcg gggctgtgcc
     4741 tgtcaggtgg gctgtggcgg ttctggttgg tgcgcggcta cctggcggag ggccgggggc
     4801 agttggcagc ggcgcttgcg gtggcgcaag actcccctgg cgcgcggctt tcgcggcgtc
     4861 acgacccgcg gggctacgtc aaggcgaggg ccaaggcgct caacggggcc ggggtgctgg
     4921 cgggcgtgca aggggactac gagacggcca ccgctttttt cgaggagggg ttggcccttt
     4981 acaggcggct ggacgaccgg ccgaacgtcg cgggcatgct tagcaacctg ggaaacatcg
     5041 ccaagtcgca gggggactac agtagagcgc gcgctctaca ggaggaaagc ctggctataa
     5101 ggcgggaatt gaacgacagg cagggcatgg cggcctctct cgcgaacctg gggaacgtgg
     5161 cccacgcgca gggcgatttc acgtccgccc gctcgctgca tgaggagagc ctgggtatcg
     5221 ccagccagtt gggggacagt tggggcgtcg ccacgacgct gaacaacctg ggcacggtgg
     5281 cgcacgagca aggcgacctg gaggcggcgc gggccttctt cgaggagagc ctggcgatga
     5341 ggcaggcgct gggagacagg cgcggcatcg cgaagtcgct ccacaatatc gggtcggtga
     5401 tcaaggacct caaggcttac gccgacgcgg ccgcgcgatg caaggagagc ctggctatat
     5461 acagcgaact gggtgacagg accggggccg cggtgtgcct ggagagcctg gcggggatag
     5521 cgagcttgca gggccaggcc cgtcgtgccg ctctgctggg aggcgcggcg gaagctttgc
     5581 gcgaggcgct gggcaacccc atccccaccg cgcaacaggc cgagcacgag gcgcgggtcg
     5641 cagccgctcg ccggcaactg ggggaggtgc cctttgccca ggcttggaag aagggccgcg
     5701 ccctggcgct agaggaggct atcgcgctgg cgcaggggca agcttgaggc gcgaacggaa
     5761 agggtagccg actgcatgag gcggggcttg gtcctgtgat ggttgcttat tctgttgcgg
     5821 tgggttatga agggggagta tcgcccatgt gcagtagctc gaggtagagc gcggcggtcc
     5881 aggtcatgcg gggcgcgccc agcggctctc cggtatgggg gtggtagtat tcccagaagc
     5941 cgggcgtgcc gacttcgacg gcgactgctt cggctggacc gggtttggtg ggcaccagcg
     6001 gccagcatgc ggctgctatg gcgctgtccc acagccagcc gtgctggtac tggcggcggg
     6061 acggcgtggt gaactcctcg cccgcgctgt tcgcctccat gcacagcgcc gccgcccgcc
     6121 gcgcctgctc cgcgcccgcg ccttcctcga tcaccgccag cgtgcttcgc cgcaggactt
     6181 ctcccagccg tcgcgcctcg tcgtggcgtt cgcggagggc gcgtattacg agccagttgg
     6241 tcacgggcca ggaggggccg gaccagtaac gcagggggtc gaaggcgggg ctgtccgcgg
     6301 cggtggaggg g
//
LOCUS       DASRWZ010000093         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_145226, whole genome shotgun sequence.
ACCESSION   DASRWZ010000093 DASRWZ010000000
VERSION     DASRWZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145226"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1312)
                     /locus_tag="VFH60_01320"
     CDS             complement(182..1312)
                     /locus_tag="VFH60_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HET6312448.1"
                     /translation="MAAEEGIRGGRKLAMPTFIPGLQLSEAFYTEAVRPILDAHFPGL
                     EHAAALIGWGSDVLGYDTLISTDHHWGPRLLLFLPEADFEVNKATVSEALSGRLPYTF
                     RGYSTNFGTPDQIGVRLLKEVESGPVQHMVEIFTVRDFFEWYLGLDPRGEISAADWLT
                     CSEHRLLGATSGKVFHDGPGELSAAREQIAYYPPELWMYMLASQWAKIAQEEAFVGRT
                     GDVGDERGSRLIAARLVQYLMRLCFLVERRYAPYSKWFGTGFSRLRCASTLGPALDGV
                     LAASSWQEREGHLSEAYRIVAGLHNDLGVTGPLDAEVRSYHGRPYMVIFAERFVEAIR
                     EAISSEEVKALPPNIGSASQFVDSTDLLEHAEVRRRLRGVYE"
     gene            complement(1273..>2272)
                     /gene="truB"
                     /locus_tag="VFH60_01325"
     CDS             complement(1273..>2272)
                     /gene="truB"
                     /locus_tag="VFH60_01325"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET6312449.1"
                     /translation="SAQDDKGGFGSVVQTAEHSRTRHSNPHYDAHLKHMDGIFNIDKA
                     LGMTSHDVVARVRRITRQRKAGHAGTLDPMATGVLPVVLGKATRLVEYLADADKAYRA
                     VVMLGATSDTYDREGTLTPTPGALMPSREQVEAALGRFRGEIEQVPPMHSAIKVGGKK
                     LYELARAGVEVERQARRVTIKTLDLEAFNPPTLQIFVECSKGTYIRSLAYDLGTVLGT
                     GAYLQDLTRTRHGPFSIEGAITLEGLDAAMQQDTWREALYPPEYILRGWHTHTATVEE
                     ELAIRQGKTLSLPAPVVGEREMLAAKTATGALLAVLYWDGERGVWQPKKVFAGGVS"
BASE COUNT          394 a          780 c          668 g          430 t
ORIGIN      
        1 gggacttggc aaggtgtagc ccccgatttc aatcgcgggg cgtcgcctgg caccttgtgt
       61 gacttattcg tgcacccgcg gggcgtcgcc tggcacctga tgttggttga tttgttgagc
      121 ttcgttattg gcaaggcgtc cccggcatgg cattctcaac cgccagtaac tacaccctgc
      181 ttcactcgta cacgccacgc agcctgcgcc gcacctccgc gtgctccaac aggtcggtag
      241 agtccacgaa ctggctggcc gagccgatgt tgggcggcag ggctttcact tcctcacttg
      301 aaatggcctc gcgtatagcc tctacaaagc gttctgcgaa gatgaccatg tacggcctgc
      361 catgatagct ccttacctcc gcgtcgagcg gccccgtgac gcccaggtcg ttgtgcagcc
      421 ctgccacgat gcggtatgcc tccgacaggt gcccctcgcg ctcctgccag gaacttgccg
      481 ccagcacgcc gtccagcgca ggcccaagcg tactcgcgca ccgcagccgc gagaaccccg
      541 tgccgaacca cttggagtaa ggggcgtacc tgcgctccac caggaagcac agcctcatca
      601 ggtattgcac gagccgggcc gcaataagcc tggacccccg ctcgtcgccc acgtcgccgg
      661 tgcgcccgac gaacgcttct tcctgggcaa tcttggccca ctgcgaggcc agcatataca
      721 tccacagctc ggggggataa tacgctatct gctcccgcgc cgcgctaagc tcgcccggcc
      781 cgtcatggaa cacctttccg cttgtggccc ctaacagcct gtgctcggag caggtgagcc
      841 aatccgctgc gcttatctcc ccacgcgggt caagccccag gtaccactcg aagaagtccc
      901 gcaccgtgaa aatctctacc atgtgctgca ccggcccgga ctcgacctcc ttcaacagcc
      961 tcacgcctat ctggtcgggc gtgccgaagt tggtagagta gccgcgaaac gtgtagggca
     1021 gcctgccgct caacgcctcg ctcaccgtcg ccttgttcac ctcgaaatcg gcctccggca
     1081 ggaacagcaa cagcctcggc ccccagtgat ggtcggtgga gatgagagtg tcgtagccca
     1141 gcacgtccga accccagccg atgagggcgg cggcatgttc caggccggga aagtgggcat
     1201 cgaggatagg gcgtaccgcc tcggtgtaga aggcttcgct gagttgcagg ccggggatga
     1261 aggtgggcat agctaactta cgccccccgc gaataccttc ttcggctgcc acaccccgcg
     1321 ctccccgtcc cagtacagca ccgccagtaa cgcccctgtc gccgtcttag ccgccagcat
     1381 ctcccgctcg cccaccacag gcgcgggcag ggacagcgtc ttaccctggc gaatcgccag
     1441 ctcttcttcg actgtggcgg tgtgggtgtg ccagccccgc agtatgtact caggggggta
     1501 tagggcctcg cgccaggtgt cctgctgcat cgcggcatcg agaccttcca gcgtaatcgc
     1561 cccctctatg ctgaatgggc cgtgcctggt gcgagtgagg tcttgcaggt acgccccggt
     1621 gcccaggacg gtaccgaggt cgtaagccag ggagcgaata taggtgccct tgctgcactc
     1681 cacgaagatt tgcagcgtag gagggttgaa ggcctcaagg tccagcgtct tgatcgtcac
     1741 ccggcgtgcc tgacgctcca cctccacccc cgcccgcgca agctcgtaca gcttcttgcc
     1801 acccaccttg atggccgagt gcatcggcgg cacctgctct atctcgcccc tgaaccgccc
     1861 taacgccgcc tccacttgtt cgcgggacgg catgagcgcg cctggcgtgg gcgtgagcgt
     1921 accctcgcgg tcgtaggtat cagacgtggc acccagcatg accaccgcgc ggtaagcctt
     1981 gtcggcatcg gccaggtact ccaccagccg ggtggcctta cccagcacga ccgggagtac
     2041 ccccgtcgcc atagggtcga gcgtgcccgc gtgccccgcc ttgcgctgcc tcgtgatgcg
     2101 ccggacgcgc gcaacgacat catgagaggt catgccaagg gctttgtcga tgttgaagat
     2161 tccatccatg tgctttagat gtgcatcgta atgagggttg ctatgcctgg tgcgtgaatg
     2221 ttcggcggtt tgcactacgg aaccaaagcc ccccttgtca tcctgagcgg ag
//
LOCUS       DASRWZ010000094         6761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_487802, whole genome shotgun sequence.
ACCESSION   DASRWZ010000094 DASRWZ010000000
VERSION     DASRWZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487802"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..1024
                     /locus_tag="VFH60_01330"
     CDS             476..1024
                     /locus_tag="VFH60_01330"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET6312450.1"
                     /translation="MANIGSINELFLHELGDIYDAEHQFLKGQEEMLQGASDGTLRQM
                     ITTHIEETRQQIAIIEQVYGLLGKQPERVMCDGAKGIVSEGQKVLRETAGASGVRDVA
                     IASAASRVEHYEISAYRGLIQGAQLMGQQQIAGLLLQNLQQEERTSQMVEQNLPVLLE
                     TAMNAEGISGGIGQIAQQHTSY"
     gene            1144..2118
                     /locus_tag="VFH60_01335"
     CDS             1144..2118
                     /locus_tag="VFH60_01335"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6312451.1"
                     /translation="MAKPLDQQVVVITGASSGIGRETALRLAKRGAKVVVAARRDEAL
                     DDLVEQIRADGGEAAAVPTDVSVYGEVEALAGAALEQYGRIDTWVNNAGVLIVGEFEK
                     IELDEARRLFDVNFWGEVHGIKAVLPIMREQGGGTIINVTSVTAQRPLPLMSIYSASK
                     SALNGLSQGLRAELEGSGIELCIVMPASIDTPLYQNALTKEGVTPKPAPPIYPPSEVA
                     KAIEKTAESPKRDVYAGPAGVLFNLNNILAPALLDKVLGAARRPAILSDRPEAEGHNN
                     LDEPLLDTPTTSSGGWRTPKYKAFDKLARVGLGVLALVVFRRLTGKHR"
     gene            2688..3389
                     /locus_tag="VFH60_01340"
     CDS             2688..3389
                     /locus_tag="VFH60_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6312452.1"
                     /translation="MSAREMTVLVVDDEPNIVEVVSAYLQREMFAVVTASDGEAALRK
                     FEEHNPDLIVLDVMLPRLDGLEVLRRVRAKGNTPVIMLTARGEESDKLVGLGIGADDY
                     LTKPFSPRELVARIKAVLRRATAAPAPEPGADTVLRFSGLKINPRTRSVETDKGPAEL
                     TAKEFDLLYFLASHSSEVFTRTQLLDQVWDYSYYGDTSTVTVHIRRLREKIEPDPMRP
                     RYVKTVWGVGYKFEK"
     gene            3601..4722
                     /locus_tag="VFH60_01345"
     CDS             3601..4722
                     /locus_tag="VFH60_01345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6312453.1"
                     /translation="MYDAIVVGARCAGSPTAMLLARKGYRVLLVDRATFPSDTMRNHF
                     VRLGGIGQLQKWGLLDRVIATGCPPVRQQIFDFGDFPMAEPTSPAGGVDAEYAPRRIV
                     LDKILVDAAGEAGAEVREGFSVSEVIFEDGRVTGIRGKSKGGATVTENAPIVIGADGL
                     HSIVAKAVGAPKYNEKPTLTCGYYSYWSGLPCNALEIFYRSEPAHLILAFPTNFELTC
                     VAVQLPSDQFAAFRLDVERNFMEILDLAPRMAERVRVGRREERFYGTGDLPNFFRKPY
                     GPGWALVGDAGYHKDPVSARGVSDAMRDAELLAEAVDAGLSGRLPLDEALAGYERQRN
                     EAARPEYAETCQTASFRPLPRRVYEGRAALRASMLNTAL"
     gene            4725..6038
                     /locus_tag="VFH60_01350"
     CDS             4725..6038
                     /locus_tag="VFH60_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6312454.1"
                     /translation="MSSGTTEPTRPRPLATLWMKVAGWALLGVAVALAVTLLYAVMRF
                     RMPGEDLLDLTQFLLVSGGISVLLGALAFRLGLGTRLPSLRIAIAAVYLVGVAVVGIN
                     VLYSAFLMFASPHDLGLLTVLLFFSAVISLFFAALLSQSMMSRLLDLLVVARRVASGD
                     LSVRARDRSPDAIGRRATELNSMVAQLEHSRKEREMLEVSRRTLIASVSHDLRTPLAS
                     MRAMVEALNDGVVSEPETVSRYLRTIQNETLHLTTLIDDLFELSQIDAGAMQLHVEPT
                     SLSDLLSDALESMSAQAAGKKIQIESHVEGELPRVPLDAPRMQRVLYNLIQNAIRHTP
                     SDGTITLTLRGQLEYVELTVADTGEGIRHADLPHIFDRFYRGEPARTRDSNSAAPGAG
                     LGLAIAKGIVEAHGGSISASSPPGAGAVFKVVLPASPGMVGTLPV"
     gene            6474..>6761
                     /locus_tag="VFH60_01355"
     CDS             6474..>6761
                     /locus_tag="VFH60_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312455.1"
                     /translation="MSNRDDDMGDMRIGASGSSSMGGAGNMGGDTGSSDMSGGSMGGS
                     TGGGMSGGGSMGGDMDDMGSGDMGGMSGGGSMGGSMGDTMGGMGSGASGGGS"
BASE COUNT         1261 a         2009 c         2222 g         1269 t
ORIGIN      
        1 acctgatcct tcgctacgct caggatgaca gagggggagt tggtcctgct ctcctgcttg
       61 cgcacggggg tagcccaatt gggggtagcg tcaggattag ttatgtcccc ttccaactat
      121 tgattggatg tggaaaactc ctacaagagg ctattgactc acgatcggct agcagctata
      181 ctcaaaccac ttcgagacgg aaggttcgcc ggaagtttcg aaggaacaga gacccggatg
      241 gtcctcacgg atcgtacggg ttttctgttt aacccccttc tgctcctctc ctgttgggcc
      301 tattcactgc ccgcctcact cccgtaatgc cgctagattt ggcgacatcc cgctgtaacc
      361 cctctatctg gcatgcccct tgcacacaag tactgcgccc gcgagcacca aagcgcggag
      421 ggcgcgggca ctcgaacaca cgaaaccgga gtagcatttc gagaggagtt gcaaagtggc
      481 aaacatcgga agcataaacg agcttttcct acacgagttg ggtgacatct atgacgccga
      541 gcaccagttc ctgaaaggcc aggaagagat gctgcaaggc gcgagcgatg gcacgctccg
      601 ccagatgatc accactcata tcgaggagac ccgccagcag atcgccatca tcgagcaggt
      661 gtacggcctc ctcggcaagc agcccgaacg cgtcatgtgc gacggggcca agggcatcgt
      721 cagcgagggc cagaaggtcc tgcgcgagac ggccggggcc agtggcgtgc gcgacgtagc
      781 tatagcgagt gccgcctcac gcgtggagca ctacgagata tcggcctatc gcggtctcat
      841 ccagggcgct cagctcatgg gccagcagca gatcgccggt ctgctcttgc agaacttgca
      901 gcaggaagag cggacctcgc agatggtcga gcagaacctg cccgtgctgc tcgaaacggc
      961 catgaacgcc gagggcatat cgggtggcat agggcagatc gcccagcagc acacgagtta
     1021 ctagcgagca atgagttatg aggactgagg actgagtagc tcatacgggc cagtagcttt
     1081 tctcacgagc tactcagtcc tcagtcctca taactcatta ctcgtaacgt taggggacgt
     1141 atcatggcaa aaccattgga ccagcaggtg gtggtgatta cgggggcttc gtcggggata
     1201 gggcgggaga ccgctttgcg gctggcgaaa cgcggagcca aggtggtcgt ggcggcgcgg
     1261 cgcgacgagg cgctggacga ccttgtggag cagatacgcg cggacggggg cgaggccgcg
     1321 gcggtgccga cggacgtgtc ggtctacggc gaggtggagg cgcttgctgg tgcggccctg
     1381 gagcagtacg ggcgcatcga cacctgggtg aacaacgccg gtgtgctgat cgtgggcgag
     1441 ttcgagaaga tcgagctgga cgaggcgcgc aggctgttcg acgtcaactt ctggggcgag
     1501 gtgcacggca tcaaggcggt gctgcccatc atgcgcgagc agggcggggg cactatcatc
     1561 aacgtcacct cggtcacggc gcaacggccc ctgcccctca tgtcgatcta ttcggcctcc
     1621 aagtcggcct tgaacggctt gagccagggg ttgcgggccg agctggaggg tagcggcata
     1681 gagctttgca tagtcatgcc cgcgagcatc gacacgcccc tctaccagaa cgcgcttacc
     1741 aaggaaggcg tgacccccaa gcccgccccg cctatctacc caccgtcgga agtggctaaa
     1801 gcgatagaga agaccgccga gtcgcccaag cgcgacgtgt acgccgggcc tgccggggtc
     1861 ctgttcaacc tgaacaacat cctcgcaccg gccctgctcg acaaggtgct gggcgcggcg
     1921 cgcagacccg ccatcctcag cgaccggccg gaggcggagg ggcacaacaa cctggacgag
     1981 ccgctgctcg atacccctac gacgtcctcc ggaggctggc gcacgccaaa atacaaggcg
     2041 ttcgacaagc ttgcaagggt ggggttggga gtgctcgcgc tggtagtatt ccgcaggctc
     2101 accggcaagc acaggtaggg gttctttggc ggcgcgcaag cgccccttcc aacaaccggc
     2161 tggatgtgta taacttcgcg atttgaggta ttgacggccc cctgatgcgt cgctatactg
     2221 aggctacttc gagatggacg gttcgccgga cgtttcggag gaacagagac ccggatggtc
     2281 ctcacggatc gtacgggttt tctgtttaac ccggactctc ctctctgctg cccattcgcc
     2341 ctgcgaggac tatgcaaaga ggagagtgaa ggccaccccc ggtcccctct tgcgagtacc
     2401 cctgcgcccc cacaactgta atactcccgt aacttctttt agctcctggt tagctcgccc
     2461 gtgtatgctt gtattcgaag gagcgggcta tccggcgtcg cgggcagcac catacccgct
     2521 gcgatgctgc cgttacgccg ggttggaatg ggtcgccgtg tgtcacacga tagggccggg
     2581 gtgctataat ggtgcaactt cacgcgagcc aacaatttcc gtgggcaagc caggtggcgg
     2641 ccctgcactc tgctgcgtgg gcgtccgcgt cgcgcgagga gatgagcttg agcgcaagag
     2701 agatgaccgt cctggtggtg gacgacgagc ctaatatagt cgaggtggtg agcgcgtatt
     2761 tgcagcgcga gatgttcgcc gtggtgaccg cctccgatgg cgaggcggcc ctgcgcaagt
     2821 tcgaggaaca caaccccgac ctgatagtgc tggacgtgat gctaccccgg ctggatgggc
     2881 ttgaggtgct gcgcagggtg cgggcgaagg gcaacacgcc ggtgattatg ctcacggcgc
     2941 ggggagagga gagcgacaag ctggtgggcc tcggcatcgg ggccgacgac tacctgacca
     3001 agcctttcag cccccgtgag cttgtggccc ggatcaaggc cgtgctgcgc cgcgccaccg
     3061 ccgcccccgc cccggagccg ggagccgata ctgtgctgcg cttctccggc ctcaagatca
     3121 acccgcgcac ccgctcggtg gagacggata aggggcctgc ggagctgacc gccaaggagt
     3181 ttgacttgct ctacttcctc gccagccact cctccgaggt attcacgcgc acccagcttc
     3241 tcgaccaggt gtgggactac tcctattacg gcgacacctc cactgtgacc gtccatatcc
     3301 gccgcctgcg cgaaaagatc gagccggacc cgatgcgtcc ccgctacgta aagacggtgt
     3361 ggggcgtggg gtacaaattt gagaagtagc actttgtagg ggacgccccg tggggacgcc
     3421 ctgccaatga attggcgggc ttaccagagg caaagtcccc ttcggggact aagcaacatg
     3481 gggtagtctc cgaaggagac ttcgtctctg gtaagcccgt ggctttagcc acagggcgtc
     3541 cccacagggc acccccttaa ggctaacgca gactgggcaa ccaacttaag gagtggcgtc
     3601 atgtacgatg cgatagtggt aggcgcgagg tgcgcaggct caccgacagc catgctgctg
     3661 gctcgcaagg gctatcgtgt gctgcttgta gaccgggcca ccttccccag cgacaccatg
     3721 cgcaaccatt tcgtgcggct tggtgggatt gggcaactcc agaagtgggg tctgctcgac
     3781 agggtgattg ccaccgggtg cccgcccgtg cgccagcaaa tatttgactt tggcgacttt
     3841 cctatggccg agcctacctc gcccgccggg ggcgtagatg ccgaatatgc ccctcgccgc
     3901 atcgtgcttg acaagatcct cgtggatgcc gcaggggagg cgggcgctga ggtacgcgaa
     3961 ggcttctcgg tgtcggaggt catctttgag gatggaaggg tcacgggtat acggggcaag
     4021 tcgaagggcg gcgcgactgt gacggagaac gcgcctatcg tcattggggc ggatgggctg
     4081 cattccatcg ttgctaaggc ggtgggcgct ccaaagtaca acgaaaagcc cacgcttacg
     4141 tgtggctatt acagttactg gagcggtctg ccctgtaatg cgctggagat attttacagg
     4201 agcgagccgg cccacctcat acttgctttt ccaacgaact tcgaactaac gtgcgtggcc
     4261 gtgcaactgc cttcagacca gttcgcggcc tttcgcctgg acgtggagcg taactttatg
     4321 gagatactgg accttgcgcc ccggatggcc gagcgggtgc gggtggggcg cagggaggag
     4381 cgtttctacg gcacgggcga cctgcctaac ttcttccgca agccgtatgg tcccggctgg
     4441 gcgctggtgg gcgatgcggg ctaccacaaa gacccggtgt cggcccgtgg cgtatccgac
     4501 gctatgagag acgccgagtt actggccgag gctgtagacg ccggcctgtc gggacgccta
     4561 ccgctcgatg aggcgctggc cgggtacgag cggcagcgca acgaggccgc caggccggag
     4621 tatgccgaga cgtgccagac cgcctctttc cggcctttgc cccgccgggt gtacgagggt
     4681 cgcgcggcgc tacgtgcaag catgttgaat accgcccttt agccatgtct tcaggcacca
     4741 cagaaccgac tcgaccccgg ccgcttgcca ctctgtggat gaaggtggcg ggctgggcgc
     4801 tgcttggggt ggcggtggcg ctggccgtta cgctgctgta cgccgtcatg cggttcagga
     4861 tgccggggga agacctgctc gacctgacgc agttcctgct cgtgtcgggg ggcatctcgg
     4921 tgctgctggg ggctttggcc ttccggttgg ggctgggcac gcggttgccc tcgttgcgca
     4981 tcgcgatagc ggcggtatac ctggtgggcg tggcggtggt gggcataaac gtgctctatt
     5041 ccgccttcct catgttcgct tcgccccacg acctgggctt gctcaccgtg cttctctttt
     5101 tctcagccgt tatctccctc ttcttcgccg cgctgctctc acagagcatg atgtcgcgtc
     5161 tgctagacct gctcgtggtg gcgcggcgcg tggcctcggg cgacctgagc gtgcgcgccc
     5221 gcgataggtc gcccgacgca ataggcaggc gcgccacgga actcaactcg atggtggcgc
     5281 agctagagca ctcccggaag gagcgggaga tgctggaagt ctcgcggcgc acgctgattg
     5341 cctcggtgtc gcacgatttg cgcacgcccc tggccagcat gcgggcgatg gtggaggccc
     5401 tcaacgacgg ggtggtgagc gagccggaga cggtatcgcg ctacctgcgc acgatacaaa
     5461 acgagacgct gcacctgacc accctcatag acgacctgtt cgagctttcg cagattgacg
     5521 cgggggcgat gcagctacat gtggagccta cttcgctgtc ggacctgctg tcggatgcgc
     5581 tggagagcat gtcggcgcag gcggcgggca agaagataca gatcgagagc cacgtggagg
     5641 gggaactgcc ccgcgtgcct ctcgacgccc cgcgcatgca gcgcgtgctg tacaacctca
     5701 tccagaacgc gatacgccac acccccagcg acggcacgat tacgctgacg ttgcggggcc
     5761 agcttgagta cgtggagctt acggtggcgg acacggggga gggcatccgg catgccgacc
     5821 tgccccacat cttcgacagg ttctaccggg gcgagcctgc ccgcacccgc gacagcaatt
     5881 cggcggcgcc gggcgcggga ctggggcttg ccatagccaa ggggatagtg gaggcgcacg
     5941 ggggtagcat aagcgccagc tcgccgccgg gcgccggggc ggtgttcaag gtggtgctgc
     6001 ccgcctcacc cggcatggtg ggcacgctgc cggtatagcc gctttgcata acacctttcc
     6061 tccaagcggc actatgtgga taacttgttc atgttgccat tgactcgctt acggagggct
     6121 gatatactca tgtcacttcg aggttatcag ttcgctggta actttggagg aacagagacc
     6181 cggatggtcc gcaaggattg tctgggtttt ctgtttaacg ctcattttcc tggcgtgggc
     6241 actctcttct caatcccctc cactaaacct ctctgtagct ctcacaggca cgccccctat
     6301 gccaatcttg gcttgcctgg cacgtcttat gcttcccttc aatggtgcgg tacgcacaaa
     6361 accgcgaccg cagagccggg tggaccttgt cgcggaggac cttaccggcg ataagcaaca
     6421 cgacgaacac gaccaacact actgggcaat gaagcccgaa ggagtgaacc aagatgagca
     6481 atcgcgatga cgatatgggt gacatgcgca taggtgcaag tggctcctct tcaatgggtg
     6541 gcgccggcaa catgggcggc gacacaggct ccagcgatat gagcggaggc tccatgggcg
     6601 gctcgaccgg aggcggcatg agcggcggag gctctatggg cggcgacatg gacgacatgg
     6661 gtagcggcga catgggcggt atgtctggtg gcggctccat gggtggttcc atgggcgata
     6721 ctatgggcgg catgggcagc ggggcgtccg gaggcggctc c
//
LOCUS       DASRWZ010000095         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_8361,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000095 DASRWZ010000000
VERSION     DASRWZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8361"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VFH60_01360"
     CDS             complement(<1..888)
                     /locus_tag="VFH60_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET6312456.1"
                     /translation="MSTFITGSVAYDNIMNFPGHFKDHILPEKIHVLNISFLVNTLKR
                     QRGGVAANIAYTMALLGRPPAVFTSVGANDWADYEAWMDRHGIDSRYVHVVPDEFTAT
                     CYITTDMDNNQITGFYTGAMAYDKQRSLHEVPRDELGYVVIGPTEPEPIVKFTRECQE
                     MGVPYIWSPIWQIIRMTPEELSEGVRGAKVVVANDYEYELIKDKTGMDQSGILEHAEM
                     VVTTKGKHGSVIMTRDEVAAIPPARPTEVVDPVGAGDAYLGGLVFALESGLDIQRAGR
                     VASLAAVYAVEQYGTQTHTY"
     gene            complement(923..2137)
                     /gene="mtnA"
                     /locus_tag="VFH60_01365"
     CDS             complement(923..2137)
                     /gene="mtnA"
                     /locus_tag="VFH60_01365"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET6312457.1"
                     /translation="MPAVWADNYMARREAMLNTVPTEQAEQEGATGLPAASHPVPPPV
                     QWLPSGAVRLVDQTRLPHHLEFLDCRTIDELTDAILRLKVRGAPAIGLAGAYGLALAA
                     HISEATGAVTLTQELQRAALTLRQTRPTAVNLPWALDTVMEVAGAAFSAGPAAIRQAV
                     LAEARRIDSENEAANLRMGEYGAELLADGMNVLTHCNAGPLAAGGIGTALGVIYTAHQ
                     QGKKLHVWVDETRPLLQGVRLTAWELGQWGIPYTVIADNMAASLMKAGRVDAVITGAD
                     RIAANGDVANKIGTYSLAVLARAHGLPYYVAAPSSTIDPHMSSGSDITIEERHADEVT
                     HHGGTRMAPQQALVYNPAFDVSPANLVSCIITESGLLRPPFEEGLRQAAALSWRARDG
                     VTMAQAVHAEGR"
     regulatory      complement(2197..2313)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT          464 a          798 c          828 g          527 t
ORIGIN      
        1 atatgtatgt gtctgcgtgc cgtactgttc taccgcgtac acggccgcca gggaagccac
       61 ccgcccggcc cgctgtatgt ccaggcccga ctccagcgcg aacaccaacc cgcctaggta
      121 cgcatcgccc gcgcccacgg ggtccactac ctctgttggc ctggcgggcg ggatggcggc
      181 gacttcgtcg cgagtcataa tgaccgagcc gtgcttgccc ttggtggtca ccaccatctc
      241 agcgtgctcc aagatgccac tctggtccat accggtcttg tccttgatta gctcgtactc
      301 gtagtcgttg gcgaccacca ccttggcacc gcgcacgccc tcgctcaatt cttccggggt
      361 catgcggata atctgccaga tgggtgacca gatgtagggc acgcccatct cctggcactc
      421 gcgggtgaac ttgacaatcg gctccggctc ggtggggcca atcaccacgt agccaagctc
      481 gtcgcggggc acctcgtgca gcgagcgttg cttgtcgtag gccatcgcgc cggtgtagaa
      541 gccggtaatc tggttgttgt ccatgtcggt ggtgatgtag caggtagcgg taaactcgtc
      601 gggcaccacg tggacgtagc ggctgtcgat gccgtgcctg tccatccatg cctcgtagtc
      661 ggcccagtcg ttcgcgccga cagaggtgaa caccgccggg ggccgaccca ggagggccat
      721 cgtgtaggcg atattggccg ccacgccgcc tcgctgccgc ttgagggtgt tgacgagaaa
      781 agagatgttg agtacgtgta tcttctccgg caggatgtgg tccttgaagt ggccggggaa
      841 gttcatgata ttgtcgtaag cgaccgaacc cgttatgaat gtgctcatct agttaccgtg
      901 attctcctct tagtgcctgt gtttaccggc cttcagcatg cacggcctgt gccatagtga
      961 ccccgtcccg cgctcgccag gaaagggccg ccgcctgccg cagaccctct tcaaacgggg
     1021 ggcgcagcaa gccggactca gtgattatac aactaaccag attggcgggc gaaacgtcga
     1081 aggcggggtt atagacgagg gcctgctgag gggccatgcg agtgcccccg tggtgggtga
     1141 cctcgtcggc gtgccgctcc tctatcgtga tgtcgctgcc gctactcatg tgcgggtcga
     1201 ttgtagagga gggtgccgct acgtaataag gcaggccgtg ggcgcgagca agcacggcga
     1261 ggctgtacgt gccgatcttg ttagccacgt cgccattggc ggcaatgcga tccgcgccgg
     1321 tgataaccgc gtccacgcgc ccggctttca tgagggaagc ggccatgttg tcagcgatga
     1381 cggtatacgg gatgccccat tgcccaagct cccaggcggt caggcgaacg ccctgcaaga
     1441 gcggacgggt ctcatcgacc cacacgtgca gcttcttgcc ctgctggtgt gccgtgtaga
     1501 taacgcccag ggccgtgccg atgcccccgg ccgccagcgg ccccgcgttg cagtgggtga
     1561 gcacgttcat gccgtccgcc agcagctcgg ctccatactc gcccatgcgc aggtttgccg
     1621 cctcgttctc gctgtcgatg cgccgcgcct ccgccagcac ggcctgcctg atagccgcag
     1681 ggccggcact gaaagccgcc cccgccacct ccataaccgt gtcgagcgcc cagggcaaat
     1741 tcacagcggt aggacgggtc tgccggagag tgagagccgc gcgctgcaat tcttgcgtca
     1801 gcgtcaccgc gccggtggcc tcggagatgt gagcggccag cgcaagccca taggcccccg
     1861 cgaggccaat agcgggcgcg cctcgcacct tgagcctgag tatggcatcg gtcagctcgt
     1921 ctatcgtgcg gcaatcgagg aattcgaggt ggtgaggaag gcgggtctgg tccacgaggc
     1981 ggacagcgcc ggaagggagc cactgtacgg gagggggcac ggggtgcgaa gcagcaggga
     2041 ggccggtagc gccctcctgt tccgcctgtt ctgttggtac cgtgtttagc atcgcttctc
     2101 gccgcgccat gtagttatca gcccacaccg ccggcaaaaa aaaagttctc tcggcacgag
     2161 agaactatca gcaagcagca caatcgttgc tgtggtctct catctttcag gctttcgcct
     2221 gccggatttg gcacctcctg cctggggctt ttacgcctca gacccggttg ccgggcttca
     2281 cggggccgat ccctccgccc atctggataa gagtatttta tctggttttg gtcagcaaat
     2341 ggaataatat cacaacgacc ggggggtgtc aagcaacact ggtgcggctt gcactctgtt
     2401 cagggtcggg gacccgtgcg cggaataggg catcgggtgc aaaggggacg ccctgtggct
     2461 aaagccacgg gcttaccaga gacgaagtct ccttcggaga ctacctgatg ttgcttagtc
     2521 cccgtagggg acttcgtctc tggtaagccc gctaattcat tgaggcatgg tcataattca
     2581 tgttgcacgt gtagagttgg gagaaatagc ggcaaat
//
LOCUS       DASRWZ010000096         3320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1173301, whole genome shotgun sequence.
ACCESSION   DASRWZ010000096 DASRWZ010000000
VERSION     DASRWZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173301"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFH60_01370"
     CDS             <1..508
                     /locus_tag="VFH60_01370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6312458.1"
                     /translation="SDTDLTMTNRPPTIGIDASRAIIAQRTGTEHYSASLLAALAGLP
                     EAARYEFRLYVNMRSADEARERLGFALPPNWRVVTAPFPRVWTHLRLSWEMLRRPPGV
                     LFVPSHVVPLWHPRRTVVTVHDLGYLEYPQAHTRSSRLYLHMSTLFSARAARRVIAVS
                     EATRRDLV"
     gene            518..1156
                     /locus_tag="VFH60_01375"
     CDS             518..1156
                     /locus_tag="VFH60_01375"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6312459.1"
                     /translation="MPPRKVSVVHHGRDPIFAPVDDAARQAETAAKYGVQAPYCLHVG
                     TLQPRKNLGLLVEAWDILRASMEQPPQLLLAGKRGWLYDSLFEMVRARGLGELVRFAD
                     YVERADLPALYSGALALTFPSLYEGFGLPPLEAMSCGTPVIASTATSVPEVVGDAGLL
                     LDPHDPRPWAEAVQRLSRDPSLQAELRRKGLERASHVTWERCARETLKVLVD"
     gene            complement(1409..1810)
                     /locus_tag="VFH60_01380"
                     /pseudo
     CDS             complement(1409..1810)
                     /locus_tag="VFH60_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AhpC/TSA family protein"
     gene            complement(1826..1963)
                     /locus_tag="VFH60_01385"
     CDS             complement(1826..1963)
                     /locus_tag="VFH60_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312460.1"
                     /translation="MIAVQPPAVGEQAPDFDLDTPHAGKLTLKDLTAKRTLIILQRHL
                     G"
     gene            complement(2059..3231)
                     /locus_tag="VFH60_01390"
     CDS             complement(2059..3231)
                     /locus_tag="VFH60_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET6312461.1"
                     /translation="MNTRRNRRLVLLVGLLVLAGLLVWRPNSVLGLITRPFGISVDVG
                     GGNATSIVVPDGFEVNIYASGLRGPRLLDVGPDGTLFVAERDAGRVAALPDKDGDGRA
                     DGSVTVADGLSRPNSVFVYSGTLIVGEQGQVSMLRLGQDYKATERTVLVPDLPSGGIH
                     NSKTAVVGSDGRLYVAMGSTCNVCDESDERRAAVSVYGLDGAGGTVFAKGLRNAVGLA
                     LNPWTGEVWASNNGRDLMGDDLPPETVYALKEGGDYGWPRCYAGDIPDPDFGKSGDAC
                     AGVEAPVVRMAAHMAPLGIAFYKGGNFPAPYNDSLYIALHGSWNSSSKVGYKVMRVPL
                     SEGRVAGDAVDFATGFLSTEDVVTGRPAGVAVGTDGSLFISDDKSGFIYKVSWRGK"
BASE COUNT          609 a         1120 c         1014 g          577 t
ORIGIN      
        1 ctcagatacc gacctaacca tgacaaaccg accaccaacc atcggcatag acgccagccg
       61 agccattatc gctcagcgca ccggcaccga gcactactcg gccagcctgc tggctgccct
      121 cgccgggcta cccgaagccg cccgctatga gtttcggctt tatgtaaata tgaggtctgc
      181 tgacgaggca cgggagaggc tggggttcgc cctgcccccg aactggcgag tggtgacagc
      241 acccttcccg cgcgtgtgga cgcacctgag gctgtcgtgg gagatgttgc gcaggccgcc
      301 gggcgtgctg ttcgtgccct cccacgtcgt gccgctgtgg caccccaggc gcaccgtagt
      361 caccgtgcac gacctgggct acctggagta cccgcaggcc catacgcgca gttcgcggtt
      421 gtacctgcac atgtccaccc ttttcagcgc cagggcggcc cgccgagtga tagccgtctc
      481 ggaggctacc aggcgcgacc tcgtgtagca ctatcgcgtg ccgccacgca aggtcagcgt
      541 ggtccatcat gggcgcgacc cgatctttgc ccccgtggac gatgcggcgc gccaggcgga
      601 gacggcggcc aaatacgggg tgcaagcacc gtactgcctc cacgttggca cgctgcaacc
      661 gcgtaagaac ctgggcctgc tggtcgaggc gtgggacata ttgagggcaa gcatggagca
      721 accgccccaa ctgctgctgg cgggcaagcg cggctggctg tacgacagcc tcttcgagat
      781 ggtgcgggcg cggggcctgg gagagttggt gcggtttgcc gactacgtgg agcgcgccga
      841 cctgcccgcc ctctactccg gcgcgctggc cctcaccttc ccctccctct acgaaggctt
      901 cggcctgccg cccctggaag cgatgtcctg cggcaccccc gttatagcct cgaccgcgac
      961 ttccgtgccc gaagtggtag gcgacgccgg cctcctgctc gacccccacg acccgcgccc
     1021 ctgggccgaa gccgtccaac gcctctcccg cgacccgagc ctgcaagccg agctacgccg
     1081 caagggcctg gaacgcgcct cccatgtcac ctgggaacgt tgcgcccgcg aaactttgaa
     1141 ggtgctagta gattagacgg aaccgccaag acgccaagag cctgccctga gcgaagccga
     1201 agggacgcaa aggaccacgc caagaggaag aaagaacggt tcaccgcaga gacgcagaga
     1261 gcgcagaggt tacgcagaga ttaaggtggt gtacttagtc aaatgaccag tgtacagaat
     1321 ggcgtgcacg ggtcctcttt ctaccctcca aaccataata tcttggtgtg gtccttggcg
     1381 tcttggcggt tccgtctcct aatgtccctt agacagcagc acccgcgagc acgcggacca
     1441 acgcctcagg gtcggggtgg tcggaggggg tggtggagcg gtgggcgtat agcagcttgc
     1501 cggtgtcgtc tacgatgaag tagccgccga gctgggcgac gtgcctggtg tgcgaagggg
     1561 tctcgcgcat gcgcaacgtc gccctcaaac cgggcagcca gttttgcgga cccagaagct
     1621 ccattgtatt ggcatctcta aggccgtaat cgaagtagat ttgcctgctc tcgtcagagg
     1681 ccagaggaaa aggtagctta tggcggcgcg cccaggcgag cgcccggttg ggtgggaaga
     1741 aagtcaccag gagcacttcg gccccggcgg cctggatagt ggggtaatgc gagcgcaact
     1801 gcgctacgta gtcgaggcag accgttcagc ccaggtggcg ctgcaagatg atgagcgtgc
     1861 gtttggccgt caggtccttg agggtaagct tgcccgcatg gggagtgtcc aggtcgaagt
     1921 cgggtgcctg ctcgcctact gccgggggtt gtactgcgat catgtctgct atatgcccct
     1981 ttctcacgtt gtgctcgatg ctccttactt gtacgcaatg gcggcaggta aggtttgtcc
     2041 gcctgaagca gctacttatt acttgccccg ccaggacacc ttgtagatga accccgactt
     2101 gtcgtccgag atgaacaggc tgccgtctgt gcccacggcc acacccgccg ggcggccggt
     2161 gaccacgtcc tccgtgctaa ggaaccccgt cgcgaaatcg actgcgtccc ccgccacccg
     2221 cccttcactg agaggcaccc gcatgacctt gtagccaacc ttcgagctgc tattccacga
     2281 gccgtgcagg gcgatgtaca ggctgtcgtt gtagggtgcg gggaagttgc cgcccttgta
     2341 gaaggcgata cccagtggtg ccatgtgcgc cgccatgcgc acaaccgggg cttccacgcc
     2401 cgcgcaggcg tcaccgctct tgccgaaatc ggggtcgggg atgtcgcccg cgtagcagcg
     2461 gggccaaccg tagtcgccgc cctccttcag cgcgtacacc gtctccgggg gcaggtcgtc
     2521 gcccatgagg tcgcgtccgt tgttggaggc ccacacctca cctgtccagg ggttgagggc
     2581 cagacccacc gcgttgcgca accccttcgc aaacacggtc ccgcccgcgc cgtccaggcc
     2641 gtacaccgat accgccgccc gtcgctcgtc gctctcatcg cagacgttgc aggtggaacc
     2701 catcgccacg tacaggcggc cgtcggagcc taccacggca gtcttgctgt tgtgtatgcc
     2761 cccgctcggc aggtcgggca ctaataccgt tcgctcagtg gccttgtagt cctggccgag
     2821 ccgcagcata gagacctgcc cctgctctcc cacgatgagc gtgcccgaat acacgaaaac
     2881 gctgttgggc ctggacagcc catccgccac cgtgacgctg ccgtcagccc gcccgtcgcc
     2941 gtccttgtcc ggtagcgcgg ctacacgccc ggcgtcgcgc tcggccacga acagggtgcc
     3001 atccggcccc acgtccagca ggcgcggccc ccgcaagccg ctggcgtaga tgttcacctc
     3061 aaacccatcc ggcaccacga ttgaagtagc attcccgccg cccacgtcca ccgaaatgcc
     3121 gaagggccgc gtaatcaacc ccagcacgct attaggccgc caaaccagca gcccggccag
     3181 caccaaaagc cctaccagca gcaccaaacg ccggttgcgc ctagtattca taaagaactc
     3241 ccccaaacat ccccacaacg cacatcgtgt taatattacg tacgcagcaa cacattggga
     3301 tgaagatagg ggcggttgcg
//
LOCUS       DASRWZ010000097         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_762253, whole genome shotgun sequence.
ACCESSION   DASRWZ010000097 DASRWZ010000000
VERSION     DASRWZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762253"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(433..1887)
                     /locus_tag="VFH60_01395"
     CDS             complement(433..1887)
                     /locus_tag="VFH60_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET6312462.1"
                     /translation="MKNPGTPKRPNILWICTDQQRFDTLGCYANPFVRTPNIDRLAAS
                     GVLFERCYSQSPVCTPSRVSFLTGRYPRTTRCRQNGQSIPGDEVLVTKLLADADTAGG
                     LSGKLHISACHPKVAPVAERRVDDGYAEFHWSHHPDPDWPANEYFRWLRGQGVSYGRA
                     AYGGSRYVQVSVPPRYHQTTWCAQKAIDHIEAAAGSGRPWLFSVNFYDPHHPFDPPLE
                     YLQRYLDHLDDIPLPAYTEGELLDKPVFQQIDHRAAYNMPGNYPFPDMSAEDHRLVRA
                     AYWAMIDLIDEQVGRMLKALEETGQLEDTLVVFMSDHGEMLGDHGIYLKGPYFYEPAV
                     RVPMLVSWPGMIPAGRRSGALVELVDLAPTLLEASGLPVHAGMQGRSLWPLLAGQAAE
                     HRQDVYCEYYNAMPWHKDTAPQATMLRTSRYKLVAMHGLGTGELYDLQADPTETRNLW
                     SDDGYAGVKLAMLERLCDRMAWTVDPLPLREADW"
     gene            2080..>2470
                     /locus_tag="VFH60_01400"
     CDS             2080..>2470
                     /locus_tag="VFH60_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET6312463.1"
                     /translation="MICSNCGAANQPGVNFCTRCGTRLVPPTAPQGALPPEIRPTGAA
                     TPPQYPPAQYPPQQYPPQYPQGQYPQGQYPQGPGYPPQQYPPGQYPVNQAPVKKGRSP
                     CGCCFTVFLVFILLVIGTGVLGYFLLQN"
BASE COUNT          465 a          779 c          763 g          463 t
ORIGIN      
        1 agcaacaaga cgagccatat acgcctgccc ttgaaacgat ccttccattg caacactttc
       61 tcttactcct ttcctgcccg aacgccaaaa cacatgactc aaaaaggccg tacaagtaaa
      121 gacggcgagt cctctacgta tacagcaaac cacttttgga ggagttgagc ggagccggat
      181 gggagcaaga gcaagagcgt agtggtaact atataagtag gttgtagagc ctgataccca
      241 gtatatcaca gggcatataa gaaagtacat cgctaacctg acgattcagg ctattaatcc
      301 gtgtaaaacg ttggtgattt gagccacgca acgggtcggc acccgcgcat atcaccggca
      361 caggtcgttc accgaggctc ttgttggagc aaggaaagcc gagctaccgg ctcatagcca
      421 atctcgcggg gcttaccagt ccgcctctcg cagtggcagg gggtccacgg tccaggccat
      481 acggtcgcac agccgctcca gcatcgccag cttcacgcct gcgtacccgt cgtcgctcca
      541 caggttgcgc gtttcggtgg ggtctgcttg caggtcgtag agttcgccgg tgcccagccc
      601 gtgcatagcc accagcttgt agcggctcgt gcgtagcatg gtggcctggg gagcggtgtc
      661 cttgtgccag ggcatggcgt tgtagtactc gcagtacacg tcctgcctgt gctcggcggc
      721 ctgcccggca agcagcggcc agagcgaccg cccttgcatg cccgcgtgca ccggcagacc
      781 cgaggcttcg agcaaggtag gcgcgaggtc caccagttcc accagcgcgc cgctcctgcg
      841 tccagcgggt atcatgccgg gccacgacac taacatgggc actcgcaccg caggctcgta
      901 gaagtagggg cctttcaggt agatgccgtg gtcccccagc atctcgccgt ggtccgacat
      961 gaagacgacc agcgtgtctt cgagttgccc cgtctcctcc aacgccttca gcatgcggcc
     1021 tacctgctcg tcaatcaggt ctatcatggc ccagtaggcc gcgcgcacca ggcggtggtc
     1081 ctcggcgctc atgtcgggga aggggtaatt gccgggcatg ttgtacgcgg cacggtggtc
     1141 tatctgctgg aagacaggct tgtcgagcag ctccccctcg gtgtaagcgg gcaggggtat
     1201 gtcgtccagg tggtccaggt agcgttgaag gtactccagc ggcgggtcga acgggtggtg
     1261 agggtcgtaa aagttgaccg aaaagagcca cggcctgccg gagcctgccg ccgcctcgat
     1321 gtggtctatg gccttctggg cgcaccaggt ggtctggtga tagcggggag gcacgctgac
     1381 ctgcacgtac ctggagccgc cgtatgcggc ccgcccgtag ctcacgccct gcccccgcag
     1441 ccaccggaag tactcgttag ccggccagtc ggggtcgggg tggtgcgacc agtggaactc
     1501 ggcgtagccg tcatcgacgc ggcgctccgc cacgggggct accttggggt ggcacgccga
     1561 aatatgcagc ttgccggaga ggccgcccgc cgtatccgcg tccgccagca gcttggtgac
     1621 cagcacttca tcgcccggta tcgactggcc gttctgcctg caccgcgtgg tgcgtggata
     1681 gcggcccgtg aggaagctca cccggctggg cgtgcacacg ggactctggc tgtagcagcg
     1741 ctcgaacagc acgccgctcg ctgcgagccg gtcgatgttc ggggtgcgca cgaaggggtt
     1801 ggcgtagcag ccgagcgtgt cgaaacgctg ctggtcggtg catatccaga ggatgttagg
     1861 ccgcttgggg gtgccggggt tcttcatggt tatacaggag catagtccat gcggcgcaaa
     1921 gaggcaagcg ccccattgca ttgctagcgg ttgcaattta taatagccac gacagccact
     1981 catcgtgtcc cactgctata gccgggctgc ttttgcattg aacggtaata gacccgcttc
     2041 cgaacgcacg ccagcgatgc caggcgtgga ggtaacgcga tgatttgtag caactgtggg
     2101 gccgccaacc agccgggggt gaatttctgc acccggtgcg gcacgcgact ggtgcccccc
     2161 acggccccac agggcgcttt gccccccgag atccgtccta ccggggctgc taccccgcca
     2221 caatacccgc ccgcgcagta ccctccgcag caatatcctc cccagtaccc ccagggtcag
     2281 tatccgcaag gccaataccc acagggtccg ggctacccgc cccaacaata cccaccgggc
     2341 caataccctg taaatcaggc ccccgttaag aagggccgca gcccctgcgg gtgttgcttc
     2401 acggtgttcc tggtattcat cctcctcgta atcggcacgg gcgtgctagg ctacttcctg
     2461 ctgcaaaacg
//
LOCUS       DASRWZ010000098         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_145649, whole genome shotgun sequence.
ACCESSION   DASRWZ010000098 DASRWZ010000000
VERSION     DASRWZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145649"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFH60_01405"
     CDS             complement(<1..380)
                     /locus_tag="VFH60_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312464.1"
                     /translation="MAIPLDENPHNAPPTHAASQAVVAPIAADLPLHDLHALLSEVGL
                     THDEYRQFVSQLGRQPNMVELGMAGAMWSEHCGYKHSKPLLRLFPTKGARVLQGPGEN
                     AGVVDIGDGWAVALKIESHNHPSAV"
     gene            624..1415
                     /locus_tag="VFH60_01410"
     CDS             624..1415
                     /locus_tag="VFH60_01410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6312465.1"
                     /translation="MSRSDIWANAEAYEQYVGRWSRLVAREFLDWLAVPPGSHWLDIG
                     CGTGALTEAILQHAQPATVMGVDPAEGFIAYAREHVQDRRASFRVEDATSLPSKDETF
                     GAVVSGLVLNHIPMHDVALAEMARVARGGGIVAAYVWDYAARMQMMRVFWDAAVELDP
                     AIAEMDQGRRYPICKPEPLAELFNRVGLTGVEVRAIDVATHFRDFDDYWSPFLGGQGP
                     APSYVTSLTEEKKTALRERIRSMLPIAPDGSIDLVARAWAVRATR"
     gene            complement(1443..>2724)
                     /locus_tag="VFH60_01415"
     CDS             complement(1443..>2724)
                     /locus_tag="VFH60_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6312466.1"
                     /translation="RIDLLGIKGAALHGADANRFVLVDNASNFLVEPLIDRVRARWIV
                     GNGLLFIDDPQHKAQRRLMMPAFHRKRIEHYAEVMRDSTNEMLDEWRPGQVLDIGKQM
                     HHLALVIAGRTLFSMDLGGSAHELGESVATIVKVVSNPLNIGLAQLPFDVFKLGEGAT
                     VRRALKRIDRTLRRIIAEHERGHTDTGDVVSMLVTARDEEGNRLSVEQIRDQLLTLFV
                     AGHETSANALTWAFYLLAQHPEVTSKLLAELDLKLKGEPPTAADLERLPYLEQVMKEV
                     LRLYPPAPSASRTAKEDFEWKGYTIKKGEIVTYVPFVSHRMPTQFVDPELFMPARWAP
                     DFRPQPPQYAYIPFAVGPRSCIGAGFAQMEIRLVLAMVLQRFRLDLVPNQHIMATVRT
                     TVQPEGKLLMRPHVQDNHPERSPARVTGNVVGAS"
BASE COUNT          486 a          787 c          925 g          526 t
ORIGIN      
        1 accgccgagg ggtggttgtg cgactcgatc ttgagggcca ccgcccagcc gtcgcctata
       61 tccaccacgc ccgcgttctc gcccggccct tgcagcacgc gcgcgccctt cgtcgggaag
      121 aggcgcagga ggggcttcga atgcttgtag ccgcagtgct cgctccacat agcccccgcc
      181 atgccaagct caaccatgtt gggttgccgt ccgagttgcg agacaaactg cctgtattcg
      241 tcgtgtgtga ggccgacttc agagaggagc gcgtgcagat cgtgcagagg gaggtccgcc
      301 gcgatggggg ctacaacagc ttggctggcg gcatgtgtcg ggggcgcgtt atggggattc
      361 tcgtcaagag gaatggccat cggctcctgg ggcttgagac ggttgagcgg ccgactgtac
      421 gtcaaaagta taccagattg gcagggcgag gttttacaca tcctggtgtg ggtgggaaga
      481 tttgggggtt aggatttcgc atatgagcgc atttagaaag ggcccggatt ctacggaatc
      541 tggacgggcc gcaccctgac gctgcctgca tctgctacaa tcgcctcaag caagtcatcg
      601 aagccgacct tgaggagagt tcaatgagca ggagtgatat ctgggcgaat gctgaggcat
      661 acgagcagta tgtaggcagg tggagccgct tagtcgcgag agaatttctt gattggcttg
      721 ctgtgccgcc aggtagccat tggctagata tcggctgcgg caccggcgcg ctcactgaag
      781 ctatactcca gcacgcgcag cccgcaaccg tcatgggtgt tgatcctgct gagggcttca
      841 ttgcctacgc tcgcgaacat gtgcaagaca ggcgcgcgtc gttcagagtg gaggacgcca
      901 cgtcgctgcc ctctaaggac gagacgtttg gcgcagtggt gtccggcctg gtgctgaacc
      961 acatccctat gcacgatgtc gcacttgctg aaatggcacg tgtcgccagg ggtggaggta
     1021 tcgtggctgc gtacgtctgg gactacgctg cccggatgca gatgatgcgc gtgttctggg
     1081 acgcggcagt ggagttagac ccggccatag ccgagatgga tcaaggccgc cgctacccta
     1141 tctgcaagcc ggagcctcta gctgagctat tcaatagagt gggtctgaca ggtgtcgaag
     1201 tacgcgcgat agacgtggct acccacttcc gcgattttga cgactactgg tcgccgttcc
     1261 tgggcgggca gggtccagct cccagctatg taacctcact caccgaggag aagaagacgg
     1321 ctctccgaga gagaataagg tcgatgctgc caatagcgcc ggacggcagc atcgacctgg
     1381 tggcgagagc gtgggcggtt agggcaacca ggtagtagga gtcgaaatcc caatgcccag
     1441 attcaactcg ctcccaccac attgcccgtc accctcgccg gggagcgttc ggggtggttg
     1501 tcctgtacgt gggggcgcat tagcagcttg ccctcgggct ggacggtggt acgcaccgtc
     1561 gccatgatgt gctggttggg caccaggtct aggcggaagc gttgcaggac catcgccagc
     1621 accaggcgta tctccatctg cgcgaagcca gcccctatgc aggagcgggg gccgacggcg
     1681 aacgggatgt aggcgtactg gggaggctga gggcggaagt cgggtgccca gcgcgcgggc
     1741 atgaagagtt cggggtctac gaactgcgtg ggcatgcggt ggctcacgaa aggcacgtag
     1801 gtcacaatct cgcccttctt gatcgtgtag cccttccact cgaagtcctc tttggcggtg
     1861 cggctggcgc tgggcgcggg cgggtagagg cgcagcacct ccttcatcac ctgttcgagg
     1921 tagggcagac gctccaggtc ggcggcggtg ggaggctcgc ccttgagctt gaggtccagt
     1981 tcggccagca gcttcgaggt cacttcgggg tgctgggcga gcaggtagaa ggcccaggtg
     2041 agggcgttgg cgctcgtctc gtgcccggcc acgaacaggg tgagcagttg gtcgcgtatc
     2101 tgctcgacgc tgaggcggtt gccctcctcg tcccgcgcgg ttaccagcat cgaaaccacg
     2161 tcgccggtgt cggtatgccc ccgctcgtgc tccgcgataa tgcgccttag cgtgcggtcg
     2221 atgcgcttga gggcgcggcg caccgtggcc ccctccccca gtttgaacac gtcgaacggc
     2281 agttgagcga ggccgatatt gagggggttg ctcaccacct tcacgatagt ggcgaccgac
     2341 tcgcccagct cgtgcgccga gcctcccagg tccatgctga acagcgtgcg ccccgcgatg
     2401 accagggcca ggtggtgcat ctgcttgcct atgtcgagca cctggccggg ccgccactcg
     2461 tccagcattt cattggtgga gtcccgcatc acctcagcgt aatgctcgat gcgcttacgg
     2521 tggaaggccg gcatcatcag gcggcgctgg gccttgtgct gggggtcgtc tatgaagagc
     2581 aggccgttgc ccacgatcca gcgggcgcgc acgcggtcaa tcagcggctc gacaaggaag
     2641 ttggaagcgt tgtccaccag cacgaagcgg ttggcatcgg ccccgtgcag cgccgcgccc
     2701 ttgatgccca gcaggtcgat gcga
//
LOCUS       DASRWZ010000099         4850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_899452, whole genome shotgun sequence.
ACCESSION   DASRWZ010000099 DASRWZ010000000
VERSION     DASRWZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899452"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFH60_01420"
     CDS             complement(<1..203)
                     /locus_tag="VFH60_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312467.1"
                     /translation="MSNKPSERPADEQTYRQLYSLLSWGQGVYYALTGLWAIVSIGTF
                     QKVTGPKVDTWLVKTVGVLIIVIG"
     gene            complement(605..1852)
                     /gene="galK"
                     /locus_tag="VFH60_01425"
     CDS             complement(605..1852)
                     /gene="galK"
                     /locus_tag="VFH60_01425"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET6312468.1"
                     /translation="MDEEQGGRANADEMRRMSRLRSAFQDLFRASPDFVARAPGRVNL
                     IGEHTDYNGGFVLPVAIDRTILIAARAVPASRQVEMVSANYGNRDTFDLDDITHAESE
                     ETSWSNYVRGVAWALQAEGHLELGRVPGAQFVIAGNVPEGAGLSSSAAIEIASALAFT
                     RLAGVELERVPLALTCKRAENEFVGVQSGIMDQFISALGETDSALLIDTRSLNYEVVP
                     LGFEELGYKVVAVDSAAPRTLAGSAYNQRTVECAEAMDILARELDLPRGAQLRDVHLR
                     QLESLQSKLPGLLYRRARHVVSENERTLEAVALMREGLGGGNNLRRFGELLYASHASL
                     RDDYEVTSRELDLLVELAGLSPGVVGARMTGGGFGGCTVNIVQTERLAEFERDVVEEY
                     RRRTGRNSALHVCRAVQGGSYIA"
     gene            complement(1852..3231)
                     /locus_tag="VFH60_01430"
     CDS             complement(1852..3231)
                     /locus_tag="VFH60_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET6312469.1"
                     /translation="MATPTEYTLPLLGLPGPRPEDFIWAGGVEDTFVPQTRPGHRALD
                     EYELIGHYEHWREDLALAKTLGLRALRWGIPWYRVEPERGRFDWRWVDEVLPYMVQEL
                     GITPIIDLMHYGCPFWLHREFDNPDYPAAVAAYAAEFAQRYKGLVRWYTPLNEPLVNA
                     LMCGKRGLWPPYLRGDQGYLRVMVHLAKGIVATVRALKEIDPHSIMVHVDAAGIVRAE
                     RRDLERVAAEDQLRHFICYDLITGRVDTEHALFPWLLRNGVSPADLYALASQPIDLDV
                     LGLNFYPQWSTKQVYINRQGKLAYKEIERQGLGFAEMIASYQQRYHAPVIVTETSARG
                     TDRVRARWLAAGLGAIKALRGQGVPVLGYTWFPIFTMIDWSYRLAKGPVEQYRLELGL
                     YRLGRAGEPRWAASPLVEQFRAYVADPEAAIGTLAAAPDAATEREYRYLYGGPAQVGE
                     QGATGERSN"
     gene            complement(3264..4331)
                     /locus_tag="VFH60_01435"
     CDS             complement(3264..4331)
                     /locus_tag="VFH60_01435"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET6312470.1"
                     /translation="MPDDRFSLGGHPHRRYNPLTREWVLVSPHRTKRPWQGQVEPPVP
                     EERARYDPTCYLCPGNERAGGVRNPQYEHTFVFTNDFAALLPDTPPGEYHEDGLLLAR
                     SERGTSRVVCFSPRHDLTLAEMERDDLCRVVDAWAGQYEELGAMRSIGYVQIFENRGA
                     MMGASNPHPHGQIWATEHLTVNVAREYESQLEYYREKGRSLLADYLAVELREGARIVV
                     ENEHFVALVPFWAVWPFETMVISRNPVGAITELDSDERSGLADVLKRLTTRYDNLFKV
                     SFPYSMGFHQAPTDGEPHPEWHLHAHFYPPLLRSATVRKFMVGFELLGEPQRDITPES
                     AAERLREQSEQHYGASDTATQ"
     gene            complement(4368..>4850)
                     /locus_tag="VFH60_01440"
     CDS             complement(4368..>4850)
                     /locus_tag="VFH60_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312471.1"
                     /translation="LREGPNGERIDEQVDEERMHGWAEMTRQYSSRYNGIPVILAETN
                     VGGEVENRVAWFQNIIDQTRLARANGVPVAGLTYYGAIDHVDWDSALRVRNYNINPCG
                     MWSLRWEGDKLVREPTALVDMYKRLINAPLAESVGELSDPQAAARIRAVLEPAARSRS
                     "
BASE COUNT          822 a         1583 c         1536 g          909 t
ORIGIN      
        1 cctatcacga tgatgagcac gcccaccgtc ttgaccaacc aggtgtctac tttgggccct
       61 gtgactttct ggaacgtgcc gatgctgacg atggcccaca ggccggtgag agcataatac
      121 accccctgcc cccaggacaa aagcgaatac agctgccggt aagtctgctc gtcagcaggt
      181 cgctcggatg gcttgttgct catgggatat gtattcctcg tttgcgtgac atagcgaaac
      241 ttgcgttgcc aagctccgtg gagcatcttg caacgaagag ggggaccaaa gggagtcttt
      301 gtcattccga gcgtagcgag gaatcaggtg ggcagggaag aaggggcatt gcaataccta
      361 gcgcccggtg ccgagcacga cgtaacctta ccgaggtgcc ttgccactag cctgctgcat
      421 tgcggctgag tacaactgcc acctgattcc tcgctaagct cggaatgaca aaggccactt
      481 tgctcctact ttcgttactt acttgcagga tgctccactt agccacttac accctttgat
      541 agcttctctg aactgggggc aagttgcgtg ccccccgcta gatcgccttg attctggcct
      601 cgggttacgc gatgtacgag ccaccttgca cggcccggca gacatgtagt gccgagtttc
      661 ggccggtccg ccgccggtac tcctcgacca catcccgttc gaactcggcc agacgttcgg
      721 tttgcactat gttcacggtg cagccgccga accctccccc ggtcatgcgc gcacccacca
      781 cgccgggaga taggcccgca agctccacta acaggtctag ctccctcgaa gtgacctcgt
      841 aatcatcgcg gaggctggcg tgcgaggcat agagtagctc cccgaagcgt cgcaggttgt
      901 ttccgccgcc caggccctcg cgcatcaggg ccaccgcttc cagcgtgcgc tcgttttcgc
      961 tgacgacgtg ccgggcgcgg cggtagagca gtccgggcaa cttcgattgc agcgactcca
     1021 gttgccgcaa atgcacgtca cgcagttgcg cgccacgtgg caggtcgagt tcgcgggcca
     1081 ggatgtccat cgcctcggcg cattcgacgg tccgctggtt gtaggcgcta ccggccaggg
     1141 tgcgaggggc cgcactgtcg actgccacca ctttgtagcc aagttcttcg aagccgagcg
     1201 gcaccacctc gtagttgagg ctgcgggtgt caatcaggag cgcggaatcg gtctcgccca
     1261 gggccgagat gaactggtcc atgatgccgc tctgtacgcc gacaaactcg ttttcggccc
     1321 gtttgcaggt tagggcgagc ggcacgcgct ccagttccac gccggcgagg cgtgtgaacg
     1381 ccaatgcgga agctatctcg atggcagccg agctactcag tccggccccc tccggcacgt
     1441 tgcccgcgat gacgaactgc gcgcccggca cccggcccaa ctcaaggtgc ccctcggcct
     1501 gtagcgccca cgcgacgccc cgcacgtagt tgctccacga ggtctcttcg ctttcggcgt
     1561 gcgtgatgtc gtccaggtcg aaggtgtccc ggttgccgta gttggccgac accatctcca
     1621 cctgacggct tgcgggaacg gcgcgggcgg caataaggat ggtgcggtcg atggccacgg
     1681 gcagcacaaa gccgccgttg tagtcggtgt gctcgccgat gaggtttacg cggccagggg
     1741 cgcgcgccac aaagtccggc gacgccctga aaaggtcctg gaatgcagac cgcagccgcg
     1801 acatgcgccg catctcgtcc gcgttggctc gcccgccctg ctcctcgtcc attagttgct
     1861 cctctctccc gtggcccctt gctcgcccac ctgagcgggg cctccgtaca ggtaccggta
     1921 ctcacgctct gtggccgcat ctggcgctgc cgccagcgtg ccgatggccg cctcggggtc
     1981 ggcaacataa gcgcggaact gctccaccag gggcgaggcg gcccagcgcg gctctccagc
     2041 gcggcctaac ctgtaaagcc ctagctccag cctgtattgc tcaaccggcc ccttagcgag
     2101 ccggtaactc caatctatca tcgtgaagat cgggaaccag gtgtagccaa gcaccggcac
     2161 tccctgcccg cgcaacgcct tgatagcccc aagccccgcc gccagccacc tggcgcggac
     2221 cctgtcggtg ccacgcgcgc tcgtctcggt cacaatcacc ggggcgtggt acctttgctg
     2281 gtagcttgcg atcatctccg cgaagcccag tccctgccgc tcgatttcct tgtaggctag
     2341 tttaccctgc ctgttgatgt acacctgctt ggtcgaccac tgcgggtaga aattcaagcc
     2401 cagcacgtcc aggtcgatag gttgggaggc cagtgcgtaa aggtcggccg gtgagacacc
     2461 gttgcgcaac agccagggga agagcgcgtg ctcggtatcg accctgccgg taatcaggtc
     2521 gtagcagatg aagtgccgga gctggtcctc ggcagccacc cgttcgaggt cgcgccgttc
     2581 cgctcttacg ataccggccg cgtccacgtg caccatgatc gagtgagggt ctatctcctt
     2641 aagggcacgc accgtggcga ctattccctt tgcaaggtgc accatgaccc gcaggtagcc
     2701 ctggtcgccg cgcaggtacg ggggccacag tccgcgcttg ccgcacatga gggcgttcac
     2761 cagcggttcg ttgagcggcg tataccacct gaccaacccc ttgtagcgtt gcgcgaactc
     2821 ggcggcatac gcggccaccg ctgccgggta gtccgggttg tcgaactcgc ggtgaagcca
     2881 gaaggggcaa ccgtagtgca tgaggtcgat gataggggtg atgccaagct cctgcaccat
     2941 gtagggcagc acctcgtcta cccagcgcca gtcgaacctg cccctctccg gctccacgcg
     3001 ataccagggg atgccccagc gaagcgcccg cagccccagc gtctttgcca gcgcgaggtc
     3061 ctctcgccag tgctcgtagt gacctataag ctcgtactcg tccagggcgc ggtggccggg
     3121 gcgagtctgc ggcacgaacg tgtcttcgac gccccccgcc cagatgaagt cctcgggccg
     3181 ggggccgggc aggcccagga gaggcagtgt gtattcggta ggtgtagcca tagctgttgg
     3241 tgtatttcct tcggttgtta aagttattgt gtagcagtgt cagaagcccc gtagtgctgc
     3301 tccgactgct ctcgcaggcg ctccgcggcc gattcggggg tgatatctct ttgcggctcg
     3361 cccagcagtt cgaagcccac catgaacttg cgcacggtgg ccgagcgcag caggggcggg
     3421 tagaagtgag cgtgcaggtg ccattcgggg tgaggctcac catcggtggg cgcctggtgg
     3481 aagcccatcg agtaggggaa ggacaccttg aacaggttgt cgtaccgcgt ggtgaggcgc
     3541 ttgagcacgt cggccagccc gctacgctcg tcgctgtcca gctccgtaat cgcgcccacc
     3601 gggttgcggc tgatcaccat cgtctcgaag ggccacaccg cccagaaggg cacaagagcc
     3661 acaaagtgct cgttctcgac cacgatgcgc gcgccctcgc gcaactcaac ggccaggtag
     3721 tcggccagca gcgagcggcc tttctcccgg tagtactcaa gttgcgattc atattcgcgg
     3781 gcgacgttga cggtcaggtg ctcggtcgcc cagatctgac cgtgggggtg cgggttgctc
     3841 gcgcccatca tcgcgccccg gttctcgaat atctgcacgt agccgatgga gcgcatcgcc
     3901 ccaagctcct catattgccc ggcccaggcg tccaccaccc ggcagaggtc gtcgcgctcc
     3961 atctcggcaa gcgtgaggtc atggcgcggc gagaagcaaa cgacgcggga ggtgccccgc
     4021 tcgctgcgcg ccaggaggag gccgtcctcg tggtactccc ccggcggggt atcgggcagt
     4081 agcgcggcga agtcgttggt gaaaacgaag gtgtgttcgt actgggggtt gcgtacgcca
     4141 ccggcccgct cgttgccggg gcacaggtag caggtagggt catagcgcgc gcgttcctcc
     4201 gggacgggtg gctccacctg cccttgccac ggccgcttgg tgcgatgggg ggagaccagc
     4261 acccactcgc gggtcagcgg gttgtagcgc ctgtgcgggt ggccgcccag gctgaaccgg
     4321 tcgtcaggca tcggtgcctc cacgtacggg gaccgcggca aatgcgctca agaacggctc
     4381 ctggcggcag gctcaagcac ggcgcggatg cgggcggcgg cctgcgggtc ggaaagctcg
     4441 ccaacggact ccgcgagggg cgcgttgatg aggcgcttgt acatatcgac cagcgcggta
     4501 ggctcccgca ccagcttatc accctcccac ctgaggctcc acatgccgca cgggttgatg
     4561 ttgtagttgc gcacgcggag cgcgctgtcc caatcgacgt gatcaatcgc gccgtagtag
     4621 gtaaggcccg ccacgggcac tccgttggcg cgggccaggc gtgtctggtc gatgatgttc
     4681 tggaaccagg ccacgcggtt ctccacctcg ccacctacgt tggtttcggc caggataacg
     4741 ggaatgccgt tgtagcgcga ggagtactgc cgggtcatct cggcccagcc gtgcatccgc
     4801 tcctcgtcta cctgctcgtc gatgcgctcg ccgttcggcc cctcgcgaag
//
LOCUS       DASRWZ010000100         6496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_625416, whole genome shotgun sequence.
ACCESSION   DASRWZ010000100 DASRWZ010000000
VERSION     DASRWZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625416"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFH60_01445"
     CDS             complement(<1..435)
                     /locus_tag="VFH60_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312472.1"
                     /translation="MFTYLIKRFLQSIGFILLAWFAVYTLMVVIAPPASITRTRPDGR
                     ISRVVPQELADPSTKAFDPWPLNFFLWLFDPRQPPEAGVDTIGNFTGHEEAAPPPGID
                     IGIGDFRLRGSGFLTGNFGRSTVVQPGEAVGDMLGEKWGRTVM"
     gene            complement(488..1477)
                     /locus_tag="VFH60_01450"
     CDS             complement(488..1477)
                     /locus_tag="VFH60_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6312473.1"
                     /translation="MATVLHSETHLAPAYTSQAAQFWKNFRRHKTGVVSLIVLTLVVL
                     AVIVIPAISPFDAHWAYPVDRYAPAGAVNTLTGQVHWLGSDYLGRDELVRLAVGGRIS
                     LLVALLATLLVLVFGSLVGLAAGYYGGWVDVVLMRLTDFMLALPLLPMYLFVMRALRQ
                     AADLRPLWIDDKTNATLTLVAVAGLFTLFSWMGLARLVRASVLSLRSQNFVEAARALG
                     AGNRRIILRHLLPNCVAPIVVSATFTAGDFLILEAVLAYFGYGVTDRPFPSWGNMLAP
                     VQALVFSLTNINPFEDIRGWLFLFPTLMILICVLCINYIGDALRSALDPYGDK"
     gene            1735..2073
                     /gene="trxA"
                     /locus_tag="VFH60_01455"
     CDS             1735..2073
                     /gene="trxA"
                     /locus_tag="VFH60_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET6312474.1"
                     /translation="MADTTTVEVITDTEFQDRVLEAESPVVVDFWAPWCGPCRMVSPI
                     VEELSTEYSGKVRFAKMNTDDNEATATQYGIWSIPTLIIFKEGKEVNRLVGFAPKEQL
                     KRQIDRSLAS"
     gene            complement(2245..4908)
                     /locus_tag="VFH60_01460"
     CDS             complement(2245..4908)
                     /locus_tag="VFH60_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET6312475.1"
                     /translation="MDSNPPQDMPPVKVRVHDTAPQHTVQPGDNQAIAAEALEAANKA
                     PTRRVPAWVPLAALLAVLAVLGGTTWWAITRGQGGPSSGGGRSSLPGDAVALINSSTP
                     LPSIVPAGPRDVVGDLSYEPEQDRWSAGLREAYRAMEEGRHSAAISQFSALVGDGTGA
                     ESRDALWGLAAAYWQGGQADSSIRAYTLLSQLDDPRSARAVARIAHIYEQTGREPEAV
                     SAYDSYLQRAGPARHALMLMKARLLGATQPAEDVYKALVDDNPSDLDLRDALLAWADV
                     KSRRNDHRGAMQLYDRLAALQASDPRPLLDNYGLPAAVHAANEARLAGDANLARTRLL
                     AYIKGQCGEEGATPCPDYPYGTYSAVEALIKLEPSAMLSGTVEPMMAARAAYESGYFN
                     RTIGFLDTLRQSAANAPQLAEASLLTGKAYQSSGAYESAYNWYTATVQTYPTSTFAVE
                     ANRRAGDTLRDQALWDEAMGAYQQTVSTYPGAGDETIRARLNGGVLAYRLEQRDVALG
                     LLEPVLSAGEVSPTLKSDALFWVGKVRKGQGDASWRNNVSQVPTLNPGSFYAFRAQSM
                     LDGAGDGGPLALTALESGVTAEDLGVDYGREAQERGGLLSWAAGLQPPGASTPGATSA
                     ATQATTPTQASTASGAGAANGIDGPLAQDPEMLRAASLFQMGFEAEAYGAYRILAERL
                     RDRDDGASLAHLVIYLRYNSNANIAMRVSELLAAMDAGNPLERPTLLLKIMYPTPFEE
                     LVLQETAQQEIDPLVMYALMRQESQFIPAARSHADARGLTQVIPSTGEGIAEQLGDNN
                     FSPGDLYVPHVNVRYGTYYLASNLPTFDRKLLPGLAAYNGGPGNADRWLAGSALIDPD
                     LYVERIDLFETEDYLRRVYQNYGFYRLTYGR"
     gene            5242..5499
                     /locus_tag="VFH60_01465"
     CDS             5242..5499
                     /locus_tag="VFH60_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312476.1"
                     /translation="MKIRPILGEIQSICRSLGQAQSLRSADVGDQHGLRSAYRQLVLS
                     SLAVIRDLCTDIEDALQLEIEDLAARSMGHSIEDPFDANPY"
     gene            complement(5699..6271)
                     /locus_tag="VFH60_01470"
     CDS             complement(5699..6271)
                     /locus_tag="VFH60_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET6312477.1"
                     /translation="MQASHDAVIIEGFPSVAQWYNLSCEYAAAAAVTLYWGDLVSQRD
                     FIREIPTHPNPHVGFRGDIFGPHGGIDDYGIYAEPLVPVLEKRGYNAVVFYGPVSRLK
                     GNVAAGNPVVVWITTGKYAERPGFYHTYEGETFKLVPYEHAVVVYGYDSDGVYLMDVG
                     DGGFYYTEWDSFLRRWSYFDQMSLVIFPQE"
BASE COUNT         1208 a         2017 c         2088 g         1183 t
ORIGIN      
        1 catgacggtc cggccccact tctcccctag catatcgccc acggcttcgc ccggctggac
       61 taccgtcgag cggccgaagt tgcccgtcag gaagccggag cctcttagcc gaaagtcccc
      121 gatgccgatg tcgatgcccg gaggcggcgc tgcctcttcg tgtcccgtga agttacctat
      181 ggtatcgacg ccggcttctg gaggctgccg ggggtcgaac agccagagga agaagttgag
      241 gggccagggg tcgaacgcct tcgtggaggg atcggcaagc tcctgcggca ccaccctgct
      301 gatgcgcccg tccgggcgcg ttctcgtgat cgaggcaggg ggggcaatca ccaccatgag
      361 cgtgtacacc gcgaaccagg caagcaagat gaaccctata ctctgtagga aacgctttat
      421 caggtacgtg aacatatcta gctccaatcc tcaggactgc cgtgcggccc gtgccggtcg
      481 cagttatcta cttgtcacca taggggtcga gggcgctgcg caaggcgtcg ccgatgtagt
      541 taatgcagag cacgcagatg aggatcatca gggtggggaa caggaacagc cagccccgta
      601 tgtcctcgaa cgggttgata ttggtcaggc tgaatacgag cgcctgcacg ggggccagca
      661 tgttgcccca ggaggggaac ggcctgtcgg tgacgccata cccgaaatag gcgaggacgg
      721 cttcgaggat gaggaagtcg cccgccgtga aggtggcgct cacgacaatg ggcgctacac
      781 agttgggcag caggtgcctc aagatgatgc gtcggttgcc cgcgcccagc gcccgcgccg
      841 cctccacgaa gttttgcgag cgcagagaca gcaccgaagc cctcaccagc cgggccaggc
      901 ccatccagct gaacagggta aacagcccgg ctaccgccac cagcgtcagg gtggcgttgg
      961 tcttgtcgtc tatccataat gggcgcaagt cagctgcttg cctgagcgcc ctcatcacga
     1021 acaggtacat cggcagcaga ggcagggcca gcatgaagtc cgtcagccgc atcaacacca
     1081 cgtctaccca gccgccgtag tagcccgccg caagccccac gagcgagccg aaaacgagca
     1141 ccagcaaggt ggcgagtagc gccaccagca atgatatgcg cccgccgacg gcgagcctga
     1201 ccagttcgtc ccggcccaga taatcggagc caagccagtg tacctgcccg gtcagcgtgt
     1261 tcacagcacc cgcgggggcg tacctgtcta ccgggtaggc ccagtgcgca tcgaacgggg
     1321 agattgccgg gatgacgatc accgccagca ccaccagggt gagcacgatg aggcttacaa
     1381 cgcctgtctt atgcctgcga aagttcttcc aaaattgggc cgcctggctg gtataagcag
     1441 gtgccaggtg cgtctctgaa tgtaaaactg tagccatata aacctcgtag ctgaaagagt
     1501 agcagcgagt cacggggcgc gaaggcccca tggtaggatt atgatagcac aaaatcgctg
     1561 ccgcaccgga cctcgtttgg tgaggtcttc ttatccacca acttgaccga gctttgaagg
     1621 gtcggttata atgatatccg tcctcgacaa aaaaggttct atatgagggg aacctcgaga
     1681 atcgagggac cgggcgacga ggcccgatat atcagagtaa aggggaaata taagatggct
     1741 gatacaacca cagtggaagt tattaccgat accgagtttc aagatagagt gctggaggcc
     1801 gaaagccccg tcgtggtgga cttttgggca ccctggtgcg ggccttgccg catggtaagc
     1861 cccatagtag aggaactgag caccgagtac agcggcaaag tgcgcttcgc caagatgaac
     1921 accgacgaca acgaggccac cgccacgcag tacggcatct ggagcatccc gaccctcatc
     1981 atcttcaagg agggcaagga agtcaaccgc ctggtaggct tcgcccccaa agagcagctc
     2041 aagcgccaga tcgaccgctc cctggcttca taacgggcga gacggaaccg ccaagacgcc
     2101 aagagcgcca agagcgccaa gattaactag gtgttctaag caaaaggacc agggtacaaa
     2161 gtatgtaccc tggtcctctt tttatcctct aaacctctat aatcttggcg ctcttggcgt
     2221 cttggcggtt ccgtctcctg tgcatcagcg gccgtatgtc agcctgtaga agccgtagtt
     2281 ctggtagacc ctacgcaggt agtcctccgt ctcgaacagg tcgatgcgct cgacgtagag
     2341 gtcgggatcg atcagggcgg agcctgcgag ccagcggtcg gcgttgccgg ggccaccgtt
     2401 ataggcggcg aggccgggca gcagcttgcg gtcgaaggtg ggcaggttgc tcgccaggta
     2461 ataggtgccg tagcggacat tgacgtgggg cacgtacagg tcgcccggcg agaaattgtt
     2521 gtcgcctagc tgctcggcta tgccctctcc ggtggagggt atcacctggg tcagccccct
     2581 cgcgtcggcg tgggagcggg ccgccgggat gaactggctc tcctgccgca tcagcgcgta
     2641 catgacgagg gggtctatct cctgttgcgc cgtctcttgc agcaccagct cctcgaaagg
     2701 cgtggggtac atgatcttga gcagcaaggt gggacgctcg agggggttgc ccgcgtccat
     2761 tgcggccagc aactcgctta cccgcatggc gatattggcg ttggagttgt agcgcaggta
     2821 gatgacaagg tgcgccagcg aagccccgtc gtcccggtcc ctgagtcgtt cggccagtat
     2881 cctatacgcg ccgtaagcct cggcctcaaa gcccatttgg aacaacgacg ccgccctcaa
     2941 catttcgggg tcttgcgcga gcggcccatc gatgccgttt gccgcacctg ccccgcttgc
     3001 tgtactggcc tgagtgggtg tggttgcctg ggtagctgcg gaggtagcgc ctggagtgct
     3061 tgcgccggga ggttgcaggc cggccgccca cgacagcagg ccgcccctct cctgcgcctc
     3121 gcggccgtag tccacgccta ggtcttccgc cgttacgccg ctctccaggg ccgttagcgc
     3181 caggggtccg ccatcgcctg cgccgtccag catcgactgc gcccggaagg cgtagaacga
     3241 gccggggttg agggtgggca cctgggagac gttgttgcgc caggaggcat cgccttgccc
     3301 cttgcgcacc ttacccaccc agaacagcgc gtccgacttg agcgtgggcg acacctcccc
     3361 cgccgagagc accggctcca acaggccgag ggcgacatcc cgctgctcca gcctgtaggc
     3421 gagcacccca ccgttcaggc gggcgcgtat ggtctcgtct cccgcgccgg ggtaggtgga
     3481 cacggtctgc tggtaagcgc ccatcgcttc gtcccagagc gcctggtcgc gcagggtgtc
     3541 gcccgcgcgg cgattggcct ccaccgcgaa ggtggaagtg gggtaggtct gcacggtggc
     3601 cgtgtaccag ttgtaggccg actcgtaagc gcccgacgac tggtatgcct tgcccgtgag
     3661 caggctggct tcggctagtt ggggcgcgtt ggcggcgctc tgccgcagcg tgtcgaggaa
     3721 ccctatcgtc cggttgaagt agcccgactc gtaggccgcc cgcgccgcca tcatcggctc
     3781 gacggtgccg ctcaacatgg cggaaggctc taacttgata agggcctcca ccgcgctgta
     3841 ggtgccgtag ggatagtcgg ggcagggggt cgcgccctct tcgccgcact gccccttgat
     3901 gtaggcgagc aggcgggtcc tggcgaggtt ggcgtcgccc gccagcctgg cctcgttggc
     3961 ggcgtgcacg gcggcgggca ggccgtagtt gtcgagcagg gggcgcgggt cggaggcttg
     4021 cagggcggcg aggcggtcgt acaattgcat ggcccctctg tggtcgttgc ggcgcgactt
     4081 cacatcggcc cacgccagca gcgcgtcgcg caggtcgagg tccgaggggt tgtcgtccac
     4141 cagcgccttg tatacgtcct cggcgggctg ggtagccccc agcagccggg ccttcatcag
     4201 catgagggcg tggcgggcgg gtccggcgcg ttgcaggtag ctgtcgtagg cgctcaccgc
     4261 ctcaggctcc cgcccggtct gctcgtagat gtgggctata cgggccaccg cgcgggcgga
     4321 gcgtgggtcg tcaagctggg ataggagcgt ataagcgcgt atcgaggagt cggcctgccc
     4381 gccctgccag tacgccgcgg ccagccccca cagcgcatcg cgggactccg ccccagtgcc
     4441 gtcgcccacc agggccgaga actgcgagat ggcggcgctg tgccggccct cctccatcgc
     4501 ccggtaggcc tcgcgcaggc ccgcgctcca cctatcctgc tcaggctcgt atgagaggtc
     4561 gccaacaacg tccctggggc cggccggcac gatgctcggc aggggcgtgg aggaattgat
     4621 gagggctacg gcatcgccgg gcaggctcga ccggcccccg ccgctgctgg gcccgccctg
     4681 gccgcgcgta atggcccacc aggtcgtgcc gcccagcacg gccagtacgg ccaggagggc
     4741 ggcaagcggc acccaggcgg gcacccggcg cgtgggcgcc ttgtttgccg cctccagggc
     4801 ttcagccgcg atagcctggt tatcacccgg ctgaacggtg tgttgaggcg cggtatcgtg
     4861 gaccctcacc tttacggggg gcatgtcttg aggcgggttg gaatccatgg aggacctgag
     4921 gcggggaatg gagtctcgga cctgggccgc gtgcccgtgc gacaccgaca gtataggcgg
     4981 cgggcatggc cgcgtcaagc cggtggcctt gtacggttat gaactgcgca gcccggcgct
     5041 ctctctgaac ctttttagcc tatttggtgt tcataaagaa agactatcgc atatcttgat
     5101 atccgcgtcg cacaattcct cgcaattgcc ttgccttccc cagttcaccc ctacaagctg
     5161 gccccgcgtc cgccccctcc gctgtccggg tcacgctcct ggtatacaat agctctcccg
     5221 ccgccaccag gagggacgac catgaagatt aggcctattc taggcgagat ccaatcgatt
     5281 tgccgcagcc tcggccaggc gcagtcccta cgctctgccg acgttggcga ccaacatggt
     5341 ctacgctccg cttaccgcca actggtgctc tcttccctcg ccgtcatccg cgacctttgc
     5401 accgatatag aggatgccct gcaactggag atagaggacc tcgccgcccg tagtatgggc
     5461 cactccattg aggacccctt cgatgccaac ccatactgac ctggctgcgc aaagacattc
     5521 tcagagatga aaaccgcaaa ggcgcaaaga gcgcaaagta cacggaagac taaccgggtg
     5581 ttctatgcaa aagaagcagg gtacgtgctg tcgtaccctg ctctttcttg tacccgctaa
     5641 accatcctat gaactttgcg ctatttgcgc ctttgcggtt tctcctcttc ccttatattt
     5701 actcctgggg gaagatcacg agggacatct ggtcgaagta gctccagcgg cgcaggaagg
     5761 agtcccattc ggtgtagtag aagccgccgt cgccaacgtc catcaggtag acgccgtcgc
     5821 tatcgtagcc gtagactacg acggcgtgct cgtagggcac cagcttgaag gtctcgccct
     5881 cgtaggtgtg gtagaagccg gggcgctccg cgtacttgcc ggtggttatc cacaccacga
     5941 ccgggttgcc cgccgctacg ttccccttga ggcgcgatac cgggccgtag aagaccaccg
     6001 cgttatagcc gcgtttctca agcaccggca ccagcggctc ggcgtagatg ccatagtcgt
     6061 ctatgccgcc gtgcgggccg aaaatgtcgc cacggaagcc gacgtgcggg ttggggtgcg
     6121 tggggatttc acggatgaag tcgcgctggg agacgaggtc gccccagtag agggtaacgg
     6181 cggcggcggc cgcgtactcg cagctcaggt tgtaccactg ggccaccgat gggaagccct
     6241 ctatgatcac ggcatcgtga gacgcttgca ccggagcggg cggcctatac agcgcggcaa
     6301 cggtcgacag gagtatgagt atcagtaggt aacgtagttt gtgcatggac ctggacatcg
     6361 caaacatggc gctcttcctc aatgcgacct agccaatcgc gggcgtcact gcctggtgct
     6421 tatgcaagca tcatgccatt ctgcatggct gggacaagat agcgtgtaac aattgcagcg
     6481 accagggtgg cctctg
//
LOCUS       DASRWZ010000101         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_488314, whole genome shotgun sequence.
ACCESSION   DASRWZ010000101 DASRWZ010000000
VERSION     DASRWZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488314"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..961
                     /locus_tag="VFH60_01475"
     CDS             170..961
                     /locus_tag="VFH60_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6312478.1"
                     /translation="MQQPQLLTVEVSEGAARVTLNRPEVRNAFNSQLIEEIRGTFERI
                     SQGAYGEVRAVVLGGEGHSFCAGADVNWMRASLDFTQEQNVADALHMASMFDQVNRCP
                     VPVIGRVHGAALGGGVGLAAVCDIVVAAEDAVWAFSEAKLGIAPAVISPYVLAKIGRT
                     HARALFFTAERFDTARAVQIGLAHIAMPEAKLDDEIERLVCEVKTSSARAIAWAKELI
                     ATVPALPPHDAMQLTAQTIAALRVSPDGQEGLRAFLEKRKPGWAS"
     gene            949..2994
                     /locus_tag="VFH60_01480"
     CDS             949..2994
                     /locus_tag="VFH60_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET6312479.1"
                     /translation="MGKLMVFGKILIANRGEIAIRIARACLAMDIKSVAVYSDADEHA
                     LHVRSCDEAYHIGPAAALESYLSGDKLIEVARRAGCDAVHPGYGFLSENAAFARAVIQ
                     AGLTWIGPSPEAITLMGSKIEAKRLAEQAGVPVVPGYYGEDQTPARLETEAERVGYPI
                     LIKASAGGGGKGMRVVETQGDFRDALEGAKREALAAFKDDTVMLERYLTEPRHIEVQV
                     LGDHYGSIVHLGERECSIQRRHQKVVEESPSPVIDDSLRAQLTGAALKLARTAGYTNA
                     GTVEFIFQDGEFFFLEMNTRLQVEHPVTEQALNFDLVEAQIRVAGGERLWVVQEEIEF
                     EGHAIEARLYAEDPEKGFLPATGIISLLDTPYDGYGIRVDAGVSQGDEISPYYDPMIA
                     KIVASGDTRHQALTRMREMLGSMRLEGVRTNLDFLRWLFSHPQFELGNTSTRFIERYY
                     RPGAFTLVPTQVVLAAGALFVLAPTSASPDPWSSGGWRQLGHGMRANVLVEGRSYRVE
                     LSGRTQSPNGYAARVTQGEQVLFEGPVVLVVPKQSAQDSDVAGVGAVVQVRLQEDGPT
                     YMLECGYSGGAQEAYLYWESREYWVRRAPALSTERLSTSLHLTDADTLESPMPGKVLK
                     VMAAVGDSVADEQPLVIIEAMKMEFTVRAPHAGVVATVHFPEGAQVAAGDILVELEK"
     gene            2991..>3902
                     /locus_tag="VFH60_01485"
     CDS             2991..>3902
                     /locus_tag="VFH60_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET6312480.1"
                     /translation="MSNRPSLPARVTVYEVGPRDGLQNERGQVQAADKVHYIDMLSAA
                     GFSVIEATSFVNPRAVPQLADAAEVLSGISRPAGVRYPVLVPNEKGMVRALESGATDV
                     AVFTAASESFTKANINSTIAESLVNFRPVFALARQHNVSVRGYVSTAFGCPYEGAVAP
                     EKVREVALALAEMGAQELSIGDTIGVGTPNQVYDILGLLLRDLPAKRLAVHFHDTRGT
                     ALANVLAALEMGISTIDSSSGGLGGCPYAPGASGNLATEDLLYMLHGLGVQTGVQLEK
                     VVEASRFITGVLNKPPASRYLQAYLAQC"
BASE COUNT          751 a         1186 c         1292 g          673 t
ORIGIN      
        1 tcgacaagta cgaggaggaa ggcaacccct actattccac cgcccggctc tgggacgacg
       61 gcattatcga cccgctcgac acccgtatgg tgctcggcct ggggattgct gcctcagcga
      121 acgcacccat acaggagacg catttcggcg tcttcaggat gtgattacga tgcagcaacc
      181 gcagctactc accgtcgagg tcagcgaagg ggccgcccgc gtaactctca accggcccga
      241 ggtccgcaac gccttcaact cgcagctgat tgaggaaata aggggcacct tcgagcgcat
      301 cagccagggt gcgtatggcg aggtgcgggc ggtagtcctg ggcggggagg ggcattcatt
      361 ttgcgcgggc gcggacgtga actggatgcg ggccagcctc gacttcacgc aagagcagaa
      421 cgtggcggac gcgctgcaca tggcaagcat gttcgaccag gtgaaccgct gcccggtgcc
      481 cgtcatcggc agggtccacg gagcggcgct agggggcggc gtcggcctcg cagccgtctg
      541 cgacatcgtg gtagcggcgg aggacgccgt gtgggccttc tcagaggcga agctgggcat
      601 cgcgcccgcg gttatctctc cttacgtact ggcgaaaatt ggccggacgc atgcccgcgc
      661 cctgttcttc accgcagaac gcttcgacac cgcccgcgct gtccagatag gtcttgccca
      721 tatcgcgatg cccgaagcga agctggacga tgagatagag cgcctcgtct gcgaagtcaa
      781 gaccagctca gcgcgggcca tcgcctgggc taaggaactg attgccacgg tccccgcgct
      841 accgccacac gacgcgatgc aactcactgc acagacaatc gcggccctgc gtgtgagtcc
      901 ggacggccag gaaggcttgc gtgcattcct cgaaaagagg aagccagggt gggcaagctg
      961 atggtgttcg gcaagatact gattgctaac cgcggcgaga ttgccatccg tattgcccgc
     1021 gcctgcctgg cgatggacat caaatcggtg gctgtatact cggacgcgga cgagcacgcg
     1081 ctgcacgtgc gctcttgcga cgaagcctac cacatcggcc cggcagccgc gctcgaaagc
     1141 taccttagtg gagacaagtt aatcgaggtc gcaaggcgtg cgggctgcga tgccgtccac
     1201 ccaggctacg gcttcctctc tgagaacgct gccttcgcga gggccgtgat ccaggcgggc
     1261 ctcacctgga tcggacccag ccccgaagct atcacactta tgggttccaa gatcgaagcc
     1321 aagcggttgg ccgaacaagc gggcgtgccg gtggtgcccg gctattacgg cgaggaccag
     1381 actcccgcgc gtcttgagac ggaggcggaa cgagttggct accccatctt gataaaggcc
     1441 tcagcggggg gcggcggcaa gggtatgagg gtagtggaga cgcaggggga cttcagggac
     1501 gcgctggagg gggcgaagcg tgaggcgctg gccgcgttca aagacgacac cgtcatgctc
     1561 gaacgctacc tgaccgagcc acgccacatc gaggtgcaag tgctcggcga ccactacggc
     1621 agcatcgtac acctgggaga gcgtgaatgc tccatccagc gcaggcacca gaaggtggtc
     1681 gaagaatcgc cctcgccggt tatagatgac tcgttgaggg cgcaactcac cggggctgca
     1741 ctcaagttgg cccgcacagc cggttataca aatgccggga cggtcgagtt catattccag
     1801 gacggcgagt tctttttcct ggaaatgaat acccgcttgc aggtggagca ccccgttaca
     1861 gagcaagcgc tcaacttcga cctggtcgaa gcacagataa gggtggcggg cggcgagcgg
     1921 ctttgggtcg tgcaggagga aatcgagttc gaggggcacg cgatagaggc ccggctctac
     1981 gccgaggacc cggaaaaggg attcttgccc gcgaccggca ttatctcctt actggatacg
     2041 ccgtacgacg gctacgggat ccgcgtggac gccggggtct cgcagggcga tgagattagc
     2101 ccttattacg acccaatgat cgccaagatc gtggcctcgg gcgacacgcg gcaccaggcg
     2161 ctcacccgca tgcgcgagat gcttggcagc atgcggctgg aaggcgtccg caccaatctg
     2221 gatttcctgc gttggctctt ctctcaccct cagttcgagc taggtaatac ctctacccgc
     2281 ttcatcgagc ggtactaccg tccgggggcg ttcacactcg tgcccacaca ggtggtactc
     2341 gcggccggtg ccctgttcgt gttagccccc acctcggcca gcccggaccc gtggagctcc
     2401 ggcgggtggc ggcagttggg gcacggcatg cgcgccaacg tgctcgttga ggggcggtcg
     2461 taccgcgtcg agctttccgg gcggacccag tctccgaatg ggtacgcagc cagggtaacc
     2521 cagggtgagc aggtgctttt cgaaggcccg gttgtgctcg tcgttccaaa gcagtcggcg
     2581 caggatagcg acgtcgcggg agtgggtgcc gtagtccagg tgaggttgca ggaagacggg
     2641 cctacgtaca tgcttgagtg tggctactcc ggcggcgcgc aggaggctta cctctattgg
     2701 gagtcccgcg agtactgggt ccggcgtgct cccgcgctct ccacggaacg tctgagcact
     2761 tcattacacc tgacggacgc agacacgttg gaaagtccca tgcccggcaa agtgctcaaa
     2821 gtaatggctg ccgtcggtga cagcgtggct gacgaacaac cgctggtcat tatagaggcg
     2881 atgaagatgg aattcacagt gcgcgccccg cacgcgggcg tggtggcaac cgtgcacttc
     2941 cccgaaggcg cgcaagtagc agccggggac atcctggtgg agcttgagaa atgagcaaca
     3001 ggccctcgtt gcccgcccgg gtcacggtgt acgaagtcgg ccccagggac gggctgcaaa
     3061 acgagagagg ccaggtgcag gccgccgata aggtccatta catagacatg ctgagcgcag
     3121 cgggcttctc ggtcatcgaa gccacgtcgt tcgtaaaccc gcgcgccgtg ccccagttgg
     3181 ctgatgccgc cgaggtgctg agtggcatca gccggcctgc cggggtgcgc taccccgtgc
     3241 tggtgccaaa cgaaaagggc atggtgcgag cgctcgaaag cggcgcgacg gacgtagcgg
     3301 tcttcactgc cgcgtcggaa agctttacaa aggccaatat caactcgacc attgcggaat
     3361 ccctggtgaa ctttcgcccg gtgttcgcgc tcgcccggca gcacaacgtc agcgtgcgtg
     3421 gctacgtgag cactgcattc ggctgcccct acgagggcgc ggtcgcccca gaaaaggtcc
     3481 gagaggtcgc cctggcgctg gccgagatgg gcgcgcagga gctatctatt ggcgacacca
     3541 taggcgtggg cacccccaac caggtgtacg acatcctggg actgcttctg cgcgacctcc
     3601 ctgcgaaacg gcttgcggtg cacttccacg atacgcgggg gacggccctc gccaatgtcc
     3661 tggcggctct ggagatgggc ataagcacaa tcgattcctc gtcggggggg ttgggcggct
     3721 gcccttacgc gccgggggcc tcaggcaacc tggcgaccga ggacctgctg tatatgctgc
     3781 acgggctggg cgtacagacc ggcgtgcagc ttgaaaaagt ggtggaagcc tcgcgtttca
     3841 tcacgggcgt gttgaacaag ccccccgcca gccgttacct ccaggcatac ctggcacagt
     3901 gc
//
LOCUS       DASRWZ010000102         3682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_77336,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000102 DASRWZ010000000
VERSION     DASRWZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77336"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..801)
                     /locus_tag="VFH60_01490"
     CDS             complement(196..801)
                     /locus_tag="VFH60_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312481.1"
                     /translation="MTSYGPGPGGTPQGGQPEVPSGAPAPPPGPGQPNQYAPPPGYAP
                     PPGYTPPPGGPPMGTTVTAPPPPRGRSPLLIIGIIVLALVLLCGIGGALLVGGLINAT
                     QPVANAGDAFMAALRDGDYNTAYDMSAPALQQDAGDAEGLQAALGTKPVASWSFTSRN
                     ISNNQGSLEGTTTYTDGSTGTVTMALIQVGNDWKVSGVSLR"
     gene            1240..2079
                     /locus_tag="VFH60_01495"
     CDS             1240..2079
                     /locus_tag="VFH60_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312482.1"
                     /translation="MEINLGEERIIELVSPYSFNEVHERMIGKKMDAFGALTKLMQRP
                     KWDEIVISGSQMRYEPFWYGSALAHYSYDRRRRYTVEVAPEVQSVTVHGQEHEVRREK
                     TSNFSFEAMEHCVEETRRELMLDPVRGDDRDFSRYTAAPNKRDVPTLEALEQGGALVV
                     PPEVRSSFLVRRLIQALVKTFQADVVKEERIDIDSIYLYFRPVYAFEYHWVPRDKRAV
                     WEFDAVTGEVRTEGGQIKRQVTAVLENDALFDVGSDAIGTIVPGAGVAVKLGRLAARK
                     ALK"
     gene            2460..>3682
                     /locus_tag="VFH60_01500"
     CDS             2460..>3682
                     /locus_tag="VFH60_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312483.1"
                     /translation="MFRKLPGDGTKGRWRLFIVIASLLGVWACAILVRWQYEPGGQFA
                     PRTVAPNGEPRRAGGELAEYGPGSGGESAELQSLEDYWLTRISYPTGKVDSRWLLEAA
                     KEDKLVPNRVPGGIPTYRRTASGPEGPNGANAADSPLAMSTNSWTSLGPQPLQSTGCQ
                     DLVAGCYEYGHVSGRVNDIEVDPVMTNVAYIAPVGGGIWKTTNCCTAATTWVPITDDP
                     LLNTMAVDDLSIDPNDHNTIYVGTGDLNFGSFSMGSVGIYKSTDAGATWSVKGADVFG
                     PAYPNAGNGFPQYNAVGKVEADPNNSDIVVAGTKTGVYFSYDGGDNWSGPCLTNNFAS
                     QRQDVTGLILSDSGDLTTMYVAVGARGYSTTVQSNLAENGANGMYRATVPASGCPAEW
                     DSISSFGNGWPNGTS"
BASE COUNT          707 a         1153 c         1142 g          680 t
ORIGIN      
        1 cgactaccaa cggcagtact cgttcaaagc ttccattgct gagcattggt gttggcctct
       61 tcctctactc actagcctgg agtagatgag ttcgcccggt tagccccagc gcacaacctg
      121 gcgtaaaaag gcccagggaa gcgcatcctg ggccttttgg ctgccgtcac ccggcggggt
      181 acgtcccgtg cttacctacc tcaggctaac gccggacacc ttccagtcgt tgcccacttg
      241 gatgagagcc attgtcacgg tgccggtgct cccatcggtg taggtggtcg tgccctcaag
      301 gctgccctgg ttgttgctga tgttgcgcga agtgaagctc caactcgcca ctggcttagt
      361 gcccagcgcg gcttgcagcc cctccgcgtc gccggcgtcc tgctgcaatg cgggggcgga
      421 catgtcatag gccgtgttgt agtcgccatc gcgtagggcg gccatgaagg cgtcgccggc
      481 gttagctacg ggctgggtag cgttgattag tccgcccacc agcaacgcgc ctccaatgcc
      541 gcagagcagc accagggcca acacaatgat accgatgatt agcaggggcg accttccacg
      601 cggaggcggg ggtgccgtga ctgttgtccc cattggcggg ccgcccggtg gaggtgtgta
      661 acccggtggg ggcgcgtagc caggcggggg cgcatactgg ttcggctgcc ccggccctgg
      721 aggaggcgca ggcgcgccgc ttggcacctc aggttgcccg ccttgaggcg tgccaccagg
      781 ccctggtcca taactggtca tgatctcatt actcctactt taagcaatat gatgcgccgg
      841 aacctttagg ccctcaacaa catagcccgg ctgcggcaat attagcaccc gcacccccgg
      901 ctgtcaatag cgcctgctcg ccgttccagc cctgtggatt cgcctaagac cactcgctcg
      961 cgaccgcctc caaaaccacc tcttgatagt cccacccggc atcagagtta gtcaataata
     1021 cagccccacg tccccgctgc ccgttgccta agaacaatac ccagcgtgcg gtgtagctct
     1081 tcggtatcaa gacatatgtc gcaaggacct cctatgccct actattgtat cctggcaatc
     1141 gcaagcgtgc ccatcttgct acggctccta caacctggga gtcttgcgcc aacgcactat
     1201 tggcggtaga attgagcaaa gtcccgtgtg aggtgctaca tggagataaa tctcggtgag
     1261 gaacgtatca tagagcttgt gtcgccttac tcgttcaacg aggtccacga gcgcatgata
     1321 ggcaagaaga tggacgcgtt cggcgcgctc accaagctca tgcagcgccc caagtgggac
     1381 gagatcgtca tcagcggcag ccagatgcgc tacgagccgt tctggtacgg ctcggccctg
     1441 gcccactaca gctacgaccg ccgccgccgc tacaccgtcg aagtggcacc cgaggtgcag
     1501 tctgtcacgg tacacggcca ggagcacgag gtacgcaggg agaaaacgtc caacttcagc
     1561 ttcgaggcga tggagcactg cgtcgaggag acccggcgcg agttgatgct tgacccggtg
     1621 cgcggcgacg accgcgattt cagccggtac acggccgccc ccaacaagcg cgacgtaccc
     1681 acgttggagg cgctcgaaca gggtggcgca ctggtggtgc cgccggaggt tcgtagttct
     1741 ttcctggtgc gccgcctcat ccaggccctg gtgaagactt tccaggccga cgtggtgaaa
     1801 gaggagcgca tcgacatcga ttccatctat ctttacttcc ggcccgtgta tgccttcgag
     1861 taccactggg tgcccaggga caagcgcgcc gtctgggagt tcgatgcggt aaccggtgaa
     1921 gtgcgcaccg agggaggcca gattaagcgg caggtgacgg ccgtgctcga aaacgacgcg
     1981 ctgttcgacg tgggtagcga cgcgattggc accatcgtgc cgggcgcggg ggtcgcggtc
     2041 aagctcggcc ggctggccgc tcggaaggcg ttgaagtaag ccgggcaggc tgatgccctg
     2101 cgcaaccggg aagtggaccc atatgtgggc gggccgggcc ttgatatgcc tggtccgctt
     2161 ttgtatttgg cggcctcctt accacttttg tttgacagag cgacgcccac ctgggtatac
     2221 tcataggctc ttggggattt agctgtcgcg gccatgcaag gccacgagcg gaggcatttt
     2281 accccagcag tgcgctattt gtcgcaggcc cgctgccgcc ggcttcgact acaagtacaa
     2341 acacagccac gagaatgggt caaacgccat gatgtttggt ccatttcttg cattatgtgc
     2401 gccgcacgac cgcccgtaag gtacgcgggg cgtactctgt agttcagcaa ggagggaaca
     2461 tgtttcgcaa gctaccagga gacggcacca agggccgctg gcgtctgttc attgtgattg
     2521 cttcgctgct gggtgtctgg gcctgcgcca tcctggtgcg gtggcagtac gagccgggag
     2581 gccagtttgc tccgcgcacc gtagcgccga acggcgagcc tcgcagggcg ggcggcgaac
     2641 tcgctgagta cggccccggc agcggaggag agagcgccga gttgcagagc ctggaagact
     2701 actggctcac ccgcataagc tatccgacgg gcaaggtcga tagcaggtgg ctgctcgagg
     2761 cggctaagga ggacaaactg gtgcccaacc gcgtgcccgg tggcatccct acctaccgcc
     2821 gcaccgccag tggtcccgaa ggccccaacg gtgccaacgc cgccgacagc cccctcgcaa
     2881 tgagcaccaa tagctggact tcgctgggcc cgcagcccct gcagagcacc ggctgccagg
     2941 acctcgtcgc aggttgctac gagtacgggc acgtttccgg gcgcgtcaac gacatcgaag
     3001 ttgacccggt tatgactaac gtggcctata tagccccggt gggtggcggc atctggaaga
     3061 ctaccaactg ctgcacagcc gccacaacct gggtgccaat caccgacgat cctctgttga
     3121 atactatggc ggtggacgac ctgtccatcg acccgaacga ccacaacacc atctacgtgg
     3181 gcacgggcga cctgaacttc ggctccttct cgatgggtag cgtcggcatc tataagagca
     3241 ccgacgccgg ggccacctgg agcgtcaagg gcgcggacgt gttcggcccg gcgtacccca
     3301 atgcaggcaa cggcttccct cagtacaacg cggtaggcaa ggtggaggcc gaccccaaca
     3361 atagcgacat tgtggtggca ggcaccaaga ccggtgtcta cttctcctac gacggtggcg
     3421 acaactggag cggcccttgc ctcaccaaca acttcgcgag ccagcgccag gatgtcacgg
     3481 gcctcatcct cagcgactcg ggcgacctta ccactatgta cgtagcggtg ggcgcgcgtg
     3541 gctacagcac tacggtgcag agcaacctcg cggaaaacgg ggccaacggc atgtacaggg
     3601 ccaccgtgcc ggccagcggt tgcccggcgg aatgggactc cataagctcg ttcgggaacg
     3661 gttggcctaa cggcacttcc ag
//
LOCUS       DASRWZ010000103         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_625586, whole genome shotgun sequence.
ACCESSION   DASRWZ010000103 DASRWZ010000000
VERSION     DASRWZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625586"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFH60_01505"
     CDS             <1..508
                     /locus_tag="VFH60_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312484.1"
                     /translation="YSPDHFKGNTATLEVKVKRGQLRFGQVQLQVPNLFAMIWTRTRR
                     WLPFFGFWFWLLLLVASSLGRTLLWAMAGAITLLVVAEALLLLWSWHVKMLVPAERLN
                     TGRLLVKSAGGDQQQIEVKVMARPSWLRRALGWTMALVLLTLEIAGIAWIVMTVAGVP
                     FNASIAPF"
     gene            533..>2347
                     /locus_tag="VFH60_01510"
     CDS             533..>2347
                     /locus_tag="VFH60_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6312485.1"
                     /translation="MRCPNCGNENPPDYVFCDECGARLQGGEDTGVAAQPGAATDTGA
                     NVGQTGDSGGIGGVGGVGGVAPVGSYGGQPQEGQQGYGADAAYASPQGGQYDGGQAGG
                     QYEPGTGGSGPMGDAGTPGGQSTYDVQGGAGATGVADGAGATGAGLDQQATQATYGED
                     KGFGAGPSATAAAGVAGGYTGDEGAGASTPVTEDTVQAGGATGQGWQPSADDVVVYAP
                     DSQAGAEQGASMPGVVEIDDDTQNGQGAAGLAPVPSGETYSDTSAGTGAAVGGVSGGA
                     ATQEGGQGQGAWASQALTLLDRAQSAMGRGDWAAFGSGMSELRSYLTGLGATTGAAGA
                     GTAAWTASSSGSYDAPTADSTAAAGQNAQGAAGAAGAVGQHGGGAFTSSDTAADQGGT
                     YGGYNEPPYSGGGSQADVAGTPTVEPQAEAGTLDAQAPSGEESGYDAPYGGTGEADAS
                     AGMAAGDVTTGPTAGAGVGMMSQGASQAAVGGIATSTNGASDNMMARLVIISTGAELP
                     IPDQEEIMVGREDPSSGIFPDVDLTPYGGEEGGVSRRHARLLHINDDYFVEDLQSTNF
                     TKLDGQRLPAHVRERLEDGARIDFGRVATIFRRSSEDDN"
BASE COUNT          438 a          697 c          842 g          370 t
ORIGIN      
        1 ctacagcccg gatcacttta agggcaacac tgccacgctt gaggtcaaag tgaagcgggg
       61 gcagctgcgt ttcggccagg tacagttgca ggtacccaat cttttcgcta tgatatggac
      121 gcgcacgcgc agatggctgc cgttcttcgg cttctggttc tggctgttgc tcctggttgc
      181 cagctcgttg ggacgcaccc tcttgtgggc gatggcgggg gcaatcacgc tccttgtggt
      241 tgccgaggcc ctgctcttgc tttggtcctg gcatgtgaag atgctggtgc ccgccgagcg
      301 actaaacacg gggcgcttgc ttgtgaagag tgcgggcggc gaccagcagc aaatcgaggt
      361 aaaggtgatg gctcgcccat cgtggctgcg cagggcgctc ggctggacga tggcattggt
      421 gttgctgact cttgagattg cgggaatagc ctggattgta atgactgtgg cgggcgtacc
      481 atttaacgcc tccatcgccc ccttctaaaa ccaacaaacg aggagaacaa gcatgagatg
      541 ccccaattgt ggtaacgaga atcctcccga ttacgtattt tgcgacgagt gcggcgcgcg
      601 cctgcaaggg ggagaagata caggggtagc cgcccagccc ggagccgcaa ccgataccgg
      661 cgctaacgtg ggacagaccg gggactccgg tggaattggt ggcgtcggtg gcgtcggtgg
      721 cgttgctccc gtgggcagtt acggcggcca gccacaggaa ggacagcagg gctacggcgc
      781 tgatgctgca tacgcctctc cgcaaggcgg gcagtacgat ggcggccagg cagggggcca
      841 atatgagcca ggcacgggcg gcagtgggcc aatgggcgac gccggtacgc caggtggaca
      901 aagcacctac gatgtccagg gtggcgcagg cgcaacaggc gttgcagacg gggccggtgc
      961 tactggcgct ggactggacc agcaagccac ccaggccact tacggtgaag ataagggctt
     1021 cggcgcgggg ccgagcgcaa cggctgcggc cggcgtggcc ggtggctaca caggggacga
     1081 aggcgctggg gctagtacgc ccgtgacgga agacaccgtc caggcgggtg gcgctacggg
     1141 ccagggctgg cagccgagcg cggacgacgt ggtagtctac gctcccgatt cacaggcggg
     1201 cgccgaacag ggtgcctcta tgccgggcgt cgtggaaata gacgacgata cgcagaacgg
     1261 acagggtgcc gcggggctgg cccctgtgcc ttcgggtgag acctattcag acacaagtgc
     1321 gggcaccggc gctgctgtcg gcggcgttag tggcggggcc gcaacgcagg aaggcgggca
     1381 ggggcagggt gcctgggcct cccaggctct caccctgctc gatagggcgc agtcagcaat
     1441 gggccggggg gactgggccg cattcggcag cggcatgtcg gaactgcgct cctacctgac
     1501 gggccttggg gctaccaccg gcgccgcagg cgccggtact gccgcctgga ccgcaagctc
     1561 ctcaggctcc tatgacgcgc ccacagccga ttctacggcc gcagccggcc agaacgcgca
     1621 gggtgcagcc ggtgcagccg gtgcggtggg ccagcacggt ggaggcgcgt ttacgagcag
     1681 cgacactgcc gccgaccagg gtggcaccta tggcggctac aacgaacccc catactccgg
     1741 cggtggctcg caggctgacg tcgcaggcac tcctactgtc gagccgcagg ccgaagcggg
     1801 cactctggat gctcaggcac cctccggcga agagtcgggt tacgacgcac cttatggcgg
     1861 caccggagaa gccgatgcga gcgcgggcat ggcagcaggt gatgtcacca caggcccgac
     1921 cgcgggcgcg ggcgtaggca tgatgagcca gggggcgtcc caggcggcgg tgggtggtat
     1981 cgcgacctcc acgaacgggg cgtcggataa catgatggcc cgcctggtga ttatctcgac
     2041 cggcgcggag ttgcccatac ccgaccagga ggagatcatg gttgggaggg aagacccgtc
     2101 cagcggcatc ttccccgacg ttgacctcac gccctacggc ggcgaagagg gcggcgtatc
     2161 gaggaggcac gcgaggctgt tgcatatcaa cgacgactac tttgtcgagg acttgcagtc
     2221 gaccaacttc accaagctcg acggccaacg cctgcccgcc cacgtccggg aacgcctaga
     2281 ggacggcgcg aggatcgact tcggcagggt ggccaccatc ttcagaagaa gctcagagga
     2341 cgataac
//
LOCUS       DASRWZ010000104         1657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_351720, whole genome shotgun sequence.
ACCESSION   DASRWZ010000104 DASRWZ010000000
VERSION     DASRWZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351720"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VFH60_01515"
     CDS             <1..1224
                     /locus_tag="VFH60_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-lysine hydrolase"
                     /protein_id="HET6312486.1"
                     /translation="GPNVLRLLPPLVITEAQLDRALDAIERVLTGQAHGAPLTPGAGE
                     APELTSEETRLLYQALTIPSLSGQERGLAEFLCGRAREMGLEASIDEAGNLIAQAMPL
                     AAHDRSAEPVVLLGHMDTVGGVVPVRVSEGLLYGRGAVDAKGPLVAFLCAASRMRRSG
                     ELRRPVVVVGAVEEEAATSRGARAVVVRYRPSACIIGEPSGAQAVTIGYKGRLLVEYE
                     VARESSHSAGPHSTAGETAAAFWQRVREHAACWNRERAGESAFAALLPSLRGMHSQGD
                     GLVERARLTLGYRLPVGYDVGALRAELERWASEDGAVVTFSGEEAAFQTTRTTPLARA
                     FIQAIRSHGGQPTFKVKTGTSDMNVVGPHWGDNIVAYGPGDSSLDHTPNEHISLSEYL
                     HSIDVLEQVLRTLAT"
     gene            complement(1532..>1657)
                     /locus_tag="VFH60_01520"
     CDS             complement(1532..>1657)
                     /locus_tag="VFH60_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014734723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-lysine deacetylase"
                     /protein_id="HET6312487.1"
                     /translation="NIVAYGPGDSSLDHTPNEHISLSEYLHSIDVLEQVLRTLAT"
BASE COUNT          283 a          514 c          559 g          301 t
ORIGIN      
        1 ggtcccaacg tgctccgcct gctgcctccc ctcgtcatca ccgaggccca acttgaccgc
       61 gccttggacg cgatagagcg ggtgcttacc gggcaagcgc acggtgcccc tctcacccct
      121 ggagcgggag aagcccccga attaacctca gaggagacac gccttctcta ccaggcgctc
      181 accataccca gcctttccgg gcaggagcgt ggtttggccg agttcctgtg cggccgggcg
      241 cgcgagatgg gcctggaggc ttcaatcgat gaggcgggca acttgatcgc gcaggccatg
      301 cctcttgccg ctcacgaccg gagtgccgag cctgtcgtgc tgctgggaca catggacacg
      361 gtcggcgggg tggtgcctgt gcgggtgtcg gaggggctgc tgtacgggcg aggggcggtg
      421 gacgcgaaag gcccgctggt ggccttcctg tgcgcggcgt ctcgcatgag gcgttcgggc
      481 gagctgaggc gtccggtggt cgtggtaggc gcggtggagg aggaggcggc cacttcgcgg
      541 ggggcgcggg cggtggtggt ccgctaccgt ccctccgcat gcatcatcgg ggagccaagc
      601 ggcgcgcaag ccgtaacgat tggttataag gggcggctgc tggtcgaata cgaagtggcg
      661 cgcgagagca gccacagcgc cgggcctcac tccaccgcag gcgagacggc cgccgccttc
      721 tggcagcgcg tacgggagca cgccgcatgc tggaaccggg agcgcgccgg ggaatcggcg
      781 ttcgcggccc tgctgccctc gttgcgcggc atgcacagcc agggtgacgg cctggtggag
      841 cgggcgcgtc tcacgttagg ctaccgtctg cccgtgggct atgacgttgg ggcgttacgc
      901 gcagaactgg aacgctgggc ctcggaggat ggcgcggttg taaccttctc cggcgaggag
      961 gcagctttcc agaccacccg caccacaccc cttgcccgtg ccttcatcca ggctatacgc
     1021 tcacatgggg gccaacccac tttcaaggtg aagaccggca cttccgacat gaacgtggta
     1081 ggcccgcact ggggcgataa catcgtcgcc tacggccccg gcgattcgtc gctcgaccat
     1141 acccccaacg agcatatcag cttgagcgag tacctccact cgatagacgt gctggagcag
     1201 gtactgcgca ccctcgccac ctagccccct caactcctcc cagaaggcct ggtggtagtt
     1261 aagatgagac gaaaccgcca agacgccaag gacgccaaga gcgcgccaag attaaggatg
     1321 gtgtcctaag caaaaaggag cagggtacaa cattacgtac cctgattctt tttgtaccgg
     1381 cccaaccttc cttatctcag cgcgtcctct gcgccctctg cgtctctgcg gtgacccgtg
     1441 cttaaacttg gcgcgctctt ggcgtcttgg cgtcttggcg gttacgtctt atcttaactg
     1501 ccaacgtgca tactgggagg agttgaaggg gctaggtggc gagggtgcgc agtacctgct
     1561 ccagcacgtc tatcgagtgg aggtactcgc tcaagctgat atgctcgttg ggggtatggt
     1621 cgagcgacga atcgccgggg ccgtaggcga cgatgtt
//
LOCUS       DASRWZ010000105         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_831330, whole genome shotgun sequence.
ACCESSION   DASRWZ010000105 DASRWZ010000000
VERSION     DASRWZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831330"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1270
                     /locus_tag="VFH60_01525"
     CDS             260..1270
                     /locus_tag="VFH60_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6312488.1"
                     /translation="MTFVWPIMLFWLVLIPAFPAIYVWMQQRRQHLVALYGNPGLVQE
                     AAGRRVGARRHIPPLFFLVALVILIVAMARPQATVSLPRLEGSVILAFDVSGSMAADD
                     LKPSRMEAAKAAAREFVQDQPSSVQIGVVTFSDGGFTAQPPTDDQAAVLASIERLTVQ
                     RGTSLARGIEASLNVLAAGEGDKPQGLSIPRTQEAEPTATPVPKGTYTSAVIVLLTDG
                     ENNQEPDPLEAAQAAADRGVRIHTVGVGSTAGATLRIEGFSVRSRLDEETLQRISEMT
                     GGTYYNAANEEDLRAIYDNLDPELAIKPQKMEMTSLFAGAGILILILGSTLSLLWLGR
                     LP"
     gene            1308..2339
                     /locus_tag="VFH60_01530"
     CDS             1308..2339
                     /locus_tag="VFH60_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6312489.1"
                     /translation="MSLLWPGFLLLLGLVPLFIGVYVWTLRRRRVALRYSSLSLVRAA
                     LPRYSHLRRHLPFALFVLALSSLIIALGRPVQIVSVPSGQATIILALDASGSMRQNDI
                     PPSRLQAAQAAALSFIQQQKSSTQIGLVAFAGYSQLIQPPTSNQEALEVAVQSLTTAR
                     GTAIGSGILESLDAIAEIDSSVAPSVDDFSSGPQPTPVPKGAYAPHIIVLLTDGVATT
                     GPYPHDAAQQVPDPWVRVYTIGFGTEAGSPGFGPGGGFGGGRQQSGGGGFRRGIDEET
                     LKQIAEMTDGEYYSASSADELLKVFQELPTYLITRHEVMEISVLFAAIGAVLVAGAMG
                     LAMRWHPLP"
BASE COUNT          442 a          803 c          867 g          463 t
ORIGIN      
        1 gacagcgcac gagatgacgc tggattgcgg tacaaaagcc ccacattgtc atcctgagcg
       61 tagcgaagga tcaggtgggt aggccagagt cttcaacgtg gtggatgatg gcggctagag
      121 gaacattggt gctcaagtag ggagagtcaa gaacgggagg ctgatccttc gctacgctca
      181 ggatgacaag gggggctttg gttgccctta tccgagagtt agcagctact cccttgctcg
      241 atatgaagtg gacgcgcata tgacgttcgt ttggcccata atgctgttct ggctggtgct
      301 gataccggcc ttcccggcaa tatacgtgtg gatgcagcag cggcggcagc atctggtcgc
      361 gctgtacggc aaccccggac tcgtgcagga ggcagcaggc cgccgggtgg gcgcgcgccg
      421 ccacattccc ccactcttct tcctggtggc cctcgtaatc ctcatcgtgg ccatggcgcg
      481 gccccaggcc acggtgagcc tgccccggct ggagggcagc gtcatcctcg cgttcgacgt
      541 ttcgggcagc atggcggccg acgacttgaa gccgagccgg atggaggcgg ccaaggccgc
      601 ggcgcgagag ttcgtgcaag accagccgtc cagcgtgcaa atcggtgtcg tcacgttcag
      661 cgacggcggc ttcaccgcgc agcctcccac cgacgaccag gcggcggtcc tcgcctcgat
      721 agagcgcctc acggtgcagc gcggcacgtc cctggcgcgg ggcatcgagg cgtcgctgaa
      781 cgtgctcgcg gcgggtgagg gagacaagcc ccagggcctc tctatccccc gcacgcagga
      841 agccgaacct accgccacgc ccgtacccaa gggcacctat acctctgccg ttatcgtcct
      901 gctcacggac ggcgagaaca accaggagcc ggacccgctg gaggccgcgc aggccgccgc
      961 cgaccggggc gtccgcatcc acacggtcgg ggtgggcagc acggcggggg ccacgctgcg
     1021 catcgagggt ttttcggtgc gctcgcggct ggacgaggag actttgcagc ggatctcgga
     1081 gatgacgggc ggcacctatt acaacgcggc gaacgaagaa gacctgcgcg ccatctacga
     1141 caacctcgac ccggagcttg ctatcaagcc ccagaagatg gaaatgacct ctcttttcgc
     1201 gggcgcgggc atcctcatct tgatactcgg tagcacgttg tcgctcctgt ggctggggcg
     1261 cttgccatga gcgggcgagt gtgcggcatg gatagggaag gtagcagatg agcttacttt
     1321 ggcccgggtt cctgctcctg ctggggctgg taccgttgtt tatcggggtg tatgtctgga
     1381 cgttgcgcag gcggcgcgtg gcgctacgct attccagcct ttccctggtg cgtgcggccc
     1441 tgccccggta ctcgcacctg cggcggcacc tgccgtttgc cctgttcgtg ctggcgctca
     1501 gcagcttgat tatcgcgctg ggccgcccgg tgcagatagt gagcgtgccc agcggccagg
     1561 ccacgatcat cctcgcgctc gatgcctccg gtagcatgcg ccagaacgat atccccccca
     1621 gccgcctgca agctgcccag gccgccgcgc tctccttcat tcagcagcaa aaatcgagca
     1681 cgcagattgg cctggtggct ttcgccgggt actcgcagct aatccagccc ccaacctcaa
     1741 accaggaggc gttggaagtg gcggtgcaga gcctgaccac ggcgcgaggt acggccatcg
     1801 gcagcggcat cctggagtcg ctggacgcta ttgccgagat cgactccagt gtcgcgccca
     1861 gcgtggacga cttttcttcg gggcctcagc caacgccggt gcccaaggga gcctacgccc
     1921 cgcacatcat cgtcctgctc accgacgggg tagccacgac cggcccctat ccccacgatg
     1981 ccgcccagca agtccccgac ccctgggtgc gcgtgtacac catcgggttc ggcacggagg
     2041 cagggtcgcc ggggtttggt ccggggggtg gtttcggtgg gggccggcag caatcgggtg
     2101 gcggtgggtt ccgcaggggc attgacgagg agacgctgaa gcagatcgcg gagatgaccg
     2161 acggggaata ttactcggcg tcgagcgcgg acgagctttt gaaggtgttc caggagcttc
     2221 ccacgtacct gattaccagg cacgaggtaa tggaaatcag cgtgttgttc gcggcgatag
     2281 gtgccgtgct ggtggcgggg gctatgggcc tggctatgcg ctggcacccg ctgccctgag
     2341 cgcacaagag cgaagggtgg agtacttgcg gccggggtga cgacattccc gctgggccgc
     2401 tgggggacgc cctgtggggg tgccctgcca atgaattggc gggcttacca gggacaaagt
     2461 ctccttcgga gactaccctc aggtctccta gtccccgtag gggactttgc ctcttctcag
     2521 cccgccaatt tattggcagg gcgtacccac aacgcgaact aaggccgcga gagga
//
LOCUS       DASRWZ010000106         5207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_77540,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000106 DASRWZ010000000
VERSION     DASRWZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77540"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFH60_01535"
     CDS             <1..539
                     /locus_tag="VFH60_01535"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HET6312490.1"
                     /translation="ILVAALLLVAVVGPRVASAHAHLESSTPADGATVASGLTQVTVT
                     FSEEVSVDQSSAQLVSADGSAVSGATGAVDRANRNTMVITTPALADGKYTVTWRAVTE
                     DDNGITNGTFSFTVGSGGVSTTGGGATTTTGGTTGTGGASLPATGSGDARPLTLLGLL
                     LATLLLGAGTLAKRAARA"
     gene            complement(734..2524)
                     /locus_tag="VFH60_01540"
     CDS             complement(734..2524)
                     /locus_tag="VFH60_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6312491.1"
                     /translation="MSGVPRAFGLVWGTHTGLTVSMGLLSVLFGILPSASAWVSKLVI
                     DGVVEAINRGDDPGGNESWVNYVVVLVLIQFGLAAGSSLLQTVRNINQQALQELTARR
                     IQLMLMQHANRLDLSFFENPEFYDTLQQAQREAGYLPTAMVEQMFGLVRSLITFLSMV
                     ALITSLGWVVAVLALLAPIPSFIASSRYGWQGFLMSRRQSPDRRRMSYYLDLLTRDTY
                     NKEIKLFGLGDFFIKLWNEIAERFYKENRSLVKRRYMAGFTWGSLSTIVTSGTFLYVA
                     LQVIARRLTLGDLTFYSQAVGQVQSSLSSILDGLSGMYENSLYLTNLYDFLDYDPLVK
                     SPATPRKLALPLKQGLEFRNVTFTYPGKTEPALRDVSFTIKPTEAVALVGQNGAGKTT
                     IVKLLTRLYDPDAGQILLDGIDIRELDLADLHATIGVIFQDYVTYYFSARDNIGVGRL
                     GEMGNQEMVEQSAGKSGADSVIEKLPKGYDTMLGRWFDDGYQLSGGEWQKVALARAFM
                     RDAEILILDEPTASLDARAEYEIFARMKELTRGKMAVFISHRFSTVRLADRIFVLENG
                     IISEAGTHEDLLSLDGTYAELFNLQAAAYR"
     gene            complement(3102..3314)
                     /locus_tag="VFH60_01545"
     CDS             complement(3102..3314)
                     /locus_tag="VFH60_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312492.1"
                     /translation="MSDANSASVAGKGPVTNCVRCDSWTAGPGPALCANCQSEGFRLC
                     QYCGQRVSNETTPYCDQCAEEIMGTA"
     gene            complement(3379..4311)
                     /locus_tag="VFH60_01550"
     CDS             complement(3379..4311)
                     /locus_tag="VFH60_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312493.1"
                     /translation="MPETFHIRTWTKTLPSREHPEHNEDLIWSARNGVAHAMIDGMGG
                     ARRMVNGKAVGGEHAAAKIAEVLDTRLQDLPDNLSVTAARELLAVAVSEAGPKVYNEV
                     NASGRIPASEVPAGKTAQEAMAAAVMTGLVFCDGGRRAVFGQNGDTRGYLFSGGELLL
                     LTEDQDAVTLDVLDGKLSEDDAQAVQEAMDAFDGHDIGKLDEKARYYFVRRYAAFGQI
                     GDSAEPPAPVFSTIQLRPGDVILLCSDGVYANLTATEIRDGLTALDPAAALVDKADAR
                     SGERTLPDPNDLSAPYNYRAHVDDTSALVILVEW"
     gene            complement(4391..5101)
                     /locus_tag="VFH60_01555"
     CDS             complement(4391..5101)
                     /locus_tag="VFH60_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6312494.1"
                     /translation="MEVICPNCGATGQEAGKFCENCGFAIPDDAGASPQVQPLDAGST
                     QPDVSGGATSTAVGQAVAGAPPGIAAAGTSGIGGPQVVGAQFVVVRDGRPSLDEGFTI
                     TRVGEFLVGRMDPESGTQVDVDVRQWVQPFDAQGQKQYLIHRKQCYIGLTPEDEVTIR
                     SYPGYEADTLVKAPGENTFTSLQNMGTKRPARPDGSFELQLNDQIYMGDPDAVMYYLS
                     GDPTAQGNYLVLELINKS"
BASE COUNT          931 a         1672 c         1571 g         1033 t
ORIGIN      
        1 ttatcctggt ggcggcgctg ctgctggtag cggtggtagg gccacgggtc gcctcggccc
       61 atgctcacct tgaaagctcg accccggcgg atggagccac cgtagcctcc ggcctcaccc
      121 aggtcacagt caccttcagc gaggaggtga gcgtggacca gtcgagcgcg caactggttt
      181 cagccgacgg cagcgctgtg tccggggcga ccggcgcggt agacagggcc aaccgcaaca
      241 cgatggtcat caccaccccc gcgctggccg acggcaagta caccgtcacc tggagggccg
      301 taaccgagga cgacaacggc atcaccaacg gcaccttcag ctttacagta ggtagcggcg
      361 gcgtgtccac caccggcggc ggcgcgacta ccactactgg cggcaccact ggtacgggtg
      421 gcgcgtccct ccccgccaca ggctccggcg acgcccgccc gctgaccctc cttggcctcc
      481 tcctcgccac cctcctactc ggtgcgggca cgctggcaaa gcgggcagca agggcttaat
      541 ctcagcctat taattcaccg cagagacgca gaggacgctg aggacgcgca gagaagaact
      601 gaggttggta aagcaaaagg gtagcatcta ctgtacgatg ctgccctttt gctttgctaa
      661 acagtcctac tctttgcgcg tcctttgcgt cctttgcgtc ctttgcgtct ttgcgttttc
      721 gtgtcgtcgc gacctacctg taagccgctg cttggaggtt gaagagttcc gcgtaggtgc
      781 cgtcgaggga cagcaggtcc tcgtgggtgc ccgcctcgga gatgatgccg ttttcgagca
      841 cgaagatgcg gtcggccagg cgcacggtgc tgaagcggtg gcttatgaac acggccatct
      901 taccccgtgt cagctccttc atgcgggcga agatctcgta ctcggcacgg gcatcgagcg
      961 aggcggtagg ctcgtccagg atgaggattt cggcgtcccg catgaacgcg cgggccaacg
     1021 ccaccttctg ccactcccca cccgatagct ggtagccgtc atcgaaccag cggcccagca
     1081 tagtatcgta gcccttcggc agcttctcta tcaccgagtc ggccccgctc ttgcccgccg
     1141 actgctcaac catctcctgg ttgcccatct cgcccagcct gcccacgcct atgttgtcgc
     1201 gggccgagaa gtagtacgtc acgtagtcct ggaagatgac cccgatagtc gcgtgcaggt
     1261 ccgcaaggtc caactcgcgt atatctatgc cgtccaggag aatctggccc gcatcggggt
     1321 cgtacaggcg ggtgagcagc ttcactatgg tcgtcttgcc cgcgccgttc tgccccacca
     1381 gcgccaccgc ctcggtaggc tttatggtga agctaacgtc gcgcaacgca ggctccgtct
     1441 tgccggggta ggtgaaggtg acgttgcgaa actcaagtcc ctgcttgagg ggcagcgcca
     1501 gcttgcgggg agtggcgggg cttttcacca gcgggtcgta gtcgaggaag tcgtacaggt
     1561 tggtgagata gaggctgttc tcgtacatgc ccgacagccc atccaggatg ctgctcaggc
     1621 tcgattgcac ctgccccacc gcctggctgt agaaggtgag gtcgcccagc gtcagccgcc
     1681 gcgcgatgac ctgcaaagcg acgtacagga atgtgccgct tgtgacaatc gtggagaggc
     1741 tgccccaggt gaaaccggcc atgtaacggc gcttgaccag gctgcggttc tccttgtaga
     1801 agcgttccgc gatctcgttc cacagcttga tgaagaaatc gcccaggccg aacagcttga
     1861 tttccttgtt gtaggtgtcg cgggtgagca ggtcgaggta gtagctcatg cggcggcggt
     1921 cgggcgactg gcggcgcgac atgaggaagc cctgccaccc gtagcgcgag cttgcgataa
     1981 agctcgggat aggtgccagc agcgccagca ctgccaccac ccagcccagg ctcgtaatca
     2041 gggccaccat gctcagaaat gtgattagcg agcgcacaag cccgaacatc tgctcgacca
     2101 tcgcggtggg aaggtagcct gcttcccgtt gcgcttgctg aagggtgtcg tagaactccg
     2161 ggttctcgaa gaagctgagg tcaagacgat tggcgtgctg catcagcata agctggatgc
     2221 ggcgagccgt aagctcctgt agcgcctgct ggttgatgtt gcgtaccgtt tgcaggagag
     2281 agcttccggc ggccagcccg aactgtatca gcaccagcac caccacgtag ttcacccacg
     2341 actcgttgcc gccagggtcg tcaccccggt tgatagcctc caccaccccg tcgatgacca
     2401 gcttgctcac ccacgcgctc gcgctcggca ggataccgaa cagcaccgag agcaggccca
     2461 tactgaccgt gaggcccgta tgcgtgcccc acaccaggcc aaaggcgcgc ggtacgccgg
     2521 acatggtaac gcgagcgttc ttcttcaggt tctgaagccg ctcgctgaaa gtcttgggac
     2581 ctgtatcttc ttcttcgggc ttttgctgtg agggtcgaga accacggcca ttgccgccat
     2641 acatgccgcg agacccgcca tgccatcctg tttgcattct ttgtgttacc ttgcttctct
     2701 aagctgtgtg tctcttatac agcaagtata acaggtacgt gcggcggggt gccctacccg
     2761 ttcggctagc ggggtgcctg gtagttggac tagcggctgt ggtcagcaac acaggttgcc
     2821 gtcagcagta cgtgaaggca accaattttg gttgacgaga ctctgggggc ctttgtcatt
     2881 ctgagcgtag cgaagaatct cgtcctccat gcaggtgcca cccttttcaa tagcaccata
     2941 accctttctc gcaataagaa aggtggtatt tgggtgctgg aggacgagat tcttcgctac
     3001 gctcagaatg acaaaggggg cgtgcgccta aactaggaac tgtcacactc aggcgctacc
     3061 tacggtaagc tgtctcaccg accactgacc acttgtcacc gctacgccgt ccccatgatc
     3121 tcctcggcgc attggtcgca gtagggggtg gtctcgttcg acacgcgctg gccgcaatac
     3181 tggcataggc ggaagccctc ggactggcag ttagcgcaca aggcgggacc cggccccgca
     3241 gtccagctat cgcagcgcac gcagttggtc accggaccct tacccgccac ggaggcagaa
     3301 ttcgcatcgc tcatgatgtt ctcctttctc gctcttattc caggtcagcc gtacttatat
     3361 tcgcgtagca cctgccactt accactctac cagtatcacc agcgccgagg tgtcgtccac
     3421 gtgagcgcga tagttgtagg gggcggacag gtcgttgggg tcgggcaggg tgcgctcgcc
     3481 ggagcgggca tccgccttgt ccaccagcgc ggcggcgggg tctagcgcgg taaggccgtc
     3541 ccgtatctcg gtggcggtga gattggcgta cacgccgtcg ctgcacagca ggatcacgtc
     3601 accgggccgc agttgaatgg tcgaaaagac gggggctggc ggctccgccg agtcccctat
     3661 ctggccgaac gcggcataac gcctcacgaa atagtagcgc gccttctcgt ccagcttgcc
     3721 gatgtcgtgg ccgtcgaagg cgtccatcgc ctcctggacc gcctgggcgt cgtcctcgct
     3781 cagcttgcca tccagcacgt ccaacgtcac ggcgtcctgg tcctcggtca gcagcagcaa
     3841 ctccccgccc gagaagagat agccccgcgt atcgccgttc tgtccgaaca ccgccctgcg
     3901 cccaccgtcg caaaacacca ggccggtcat cacggcggcg gccatcgctt cctgcgccgt
     3961 cttccctgcg ggcacctccg aagccggaat ccgccccgaa gcgttcacct cgttgtagac
     4021 cttgggcccc gcctccgata cggccacggc cagcaactcg cgagcggcgg tcaccgagag
     4081 attgtccggc aggtcctgca accgtgtgtc cagcacctcc gcgatcttcg ccgccgcgtg
     4141 ctcgccgcca accgccttgc cgttcaccat gcgccgcgcc ccacccatgc cgtcgatcat
     4201 ggcatgggcg acgccgttcc gcgccgacca gatcaggtcc tcgttatgct cagggtgctc
     4261 cctgctgggc agggtcttgg tccaggtacg gatgtggaat gtttcgggca caaaaccccc
     4321 tggcgatttt agattttaga ttttggattc ggcaaatttc cagacaatcc aaaatctaaa
     4381 atctaaaatg ctacgatttg tttatcagct caagcacaag gtaattcccc tgtgctgtgg
     4441 gatcgccgga gaggtagtac atcacggcgt cggggtcgcc catgtatatc tggtcgttga
     4501 gttgtagctc gaacgagccg tcggggcggg cgggccgctt ggtacccatg ttctgcagcg
     4561 aggtgaaggt gttctcgccg ggcgccttca ccagcgtatc ggcttcatat cctgggtacg
     4621 agcgtatggt cacctcgtct tcgggggtga ggccgatgta gcactgcttg cggtggatga
     4681 ggtactgctt ctggccctgg gcgtcgaaag gctgcaccca ctggcgcacg tcaacgtcca
     4741 cctgcgtgcc cgactccggg tccatgcgcc ccaccaggaa ctcgcccacc cgcgtgatgg
     4801 tgaacccttc gtccaggctg ggccgcccgt cgcgcactac cacgaattga gcgcccacga
     4861 cctgcgggcc accgatcccc gaagtcccgg cagccgcgat accgggcggg gcacctgcta
     4921 ctgcctgccc caccgctgtg ctcgtcgccc cgccacttac gtccggctga gtgctacccg
     4981 catcgagagg ttgcacctgg ggagatgcgc cggcatcgtc ggggatggcg aagccgcagt
     5041 tctcgcagaa cttccccgcc tcctgcccag tagcgccgca attcggacag attacttcca
     5101 tgtacgccct ccgttgccaa acggtcttga tgagttttga gtgccgagtg ccgagtgccg
     5161 agtgccgagt gccgagtgcc gagtgccgag tgccgagtgc cgagtgc
//
LOCUS       DASRWZ010000107         4526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1242457, whole genome shotgun sequence.
ACCESSION   DASRWZ010000107 DASRWZ010000000
VERSION     DASRWZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242457"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /gene="glgX"
                     /locus_tag="VFH60_01560"
     CDS             complement(<1..1918)
                     /gene="glgX"
                     /locus_tag="VFH60_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET6312495.1"
                     /translation="MNVWPGKPYPLGATWDGTGVNFALFSENATGVELCLFDSTDDEA
                     ERMRIRMTEQTDYVWHVYMPNLKPGQLYGYRVYGPYEPSQGHRFNPAKLLLDPYAKAI
                     TGRIDWSDALFGYPMGLEDPDRDLQMDTQDSAPGMNRSVVIDPAFDWGDDKAPNTPLH
                     ESILYEVHVKGFSAQHPDIDPKLRGTYAGLATPEAIDYFRTLGITAVELLPVHHFVND
                     KTLVDKGLSNYWGYNSIGFFAPYSEYSSSGKLGEQVREFKAMVKTLHAAGIEVILDVV
                     YNHTAEGNHFGPHLSFKCIDNVSYYRLVPDDPRFYMDYTGTGNTLNMLHPRTLQTVMD
                     SLRYWVTEMHVDGFRFDLASTLARGLHEVGRLNSFLDIIHQDPVISQVKLIAEPWDIG
                     EGGYQVGNFPVNWSEWNGKYRDTVRSFWRGDESQVAELAYRLSGSSDLYQHNGRAPYA
                     SINFVIAHDGFTLNDLVSYNEKHNEANGENNMDGESHNRSWNFGVEGPTDDPEITAAR
                     EKQKRNFLATLLLSQGVPMICGGDEFGRTQQGNNNAYCQDNEISWYNWDWSDKDHDLF
                     EFTRKLIQVRKDFPVLHRRKFFQGRRIRGSEVEDLTWFRPDGQEMTEAEWGSHTTRCF
                     GMRLGGDAMMEWDDR"
     gene            complement(2077..>4526)
                     /gene="treY"
                     /locus_tag="VFH60_01565"
     CDS             complement(2077..>4526)
                     /gene="treY"
                     /locus_tag="VFH60_01565"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HET6312496.1"
                     /translation="SPYFKARAESLHGYDIANHNELNPSIGTEADLDHLPSHFETERE
                     RILERNREKEVIKRRIASLCASEPRVSAFIEGNVERFNGVQGEPHSFDLLDSLLERQP
                     YRLSFWRVATEEINYRRFFDVNDLAAVRIDQPPVFQETHRMIMRFLREGKLHGLRIDH
                     VDGLYDPARYFQDLQHSYFLEICRLVMDELGPELERTDRAELEALLVGRFDAERMADP
                     QGVLNRPLYVVVEKILARGEALPADWVVHGTTGYEFTNDANGLFVDSANARAFDEIYN
                     SIMGEKVKFADLVYESKQRIMRLSLSSEINVLTNMLNRISERDRHYRDFTLNGIRNAI
                     REVIACFPVYRTYITPETTEVERRDQAHIERAVALARKRNPAVDPSAFDFLGDILMLR
                     GFDDLPPEECAERTNFVMKFQQVTGPVIAKGLEDTAFYVYNRLISLNEVGGEPGYFGI
                     YPAAFHKAQAERQRHWPFSLLTTSTHDTKRSEDVRMRIDVLSEMPKEWRTAFRRWARA
                     NRKHKKNVEGQPAPSPNEEYFLYQTLVGVWPLEEDLGEEAHAEVVERVQAYMRKAMNE
                     AKVNTSWVNPNEAYQQAVADFIATILRRDEDNRFFADFLPFQRRVAHYGAFNSLSQVL
                     IKLTSPGVPDIYQGNEVWDFSLVDPDNRRPIDYGLRRRLLDQVEEVKEVGAVASLVNT
                     KEDGRIKLLVTSRALNFRRANLRLFQHGSYTPIAVEGKHAGNIVAYARAYEGSLAATV
                     APCLVTQLGKAGETVVPVGPVWSGSWLPLPGALDGDSFRNVFTGETLSAASRNGSVGL
                     PLEEVLAVFPVALLEKV"
BASE COUNT          724 a         1365 c         1514 g          923 t
ORIGIN      
        1 ctcggtcgtc ccactccatc atggcgtccc caccgagccg catcccgaag cagcgggtgg
       61 tgtgtgagcc ccattctgct tccgtcatct cctggccgtc cgggcggaac caggtgagat
      121 cctccacctc ggagccgcgg atgcggcggc cctggaagaa cttgcgccgg tgcagcaccg
      181 ggaagtcctt gcgcacctgg atcagcttgc gggtgaactc gaacaggtca tggtccttgt
      241 cggaccagtc ccagttgtac cagctaatct cgttgtcctg gcagtaggcg ttgttgttac
      301 cctgctgcgt ccggccaaac tcgtcgccgc cgcagatcat gggcacgccc tggctgagca
      361 ggagggtcgc caggaagttg cgcttctgct tctccctcgc ggcggtaatc tccgggtcgt
      421 cggtggggcc ttccacgccg aagttccagc tacgattgtg gctctccccg tccatgttgt
      481 tctcgccgtt cgcctcgttg tgcttctcgt tgtagctcac gaggtcgttg agcgtgaagc
      541 cgtcgtgggc gatcacgaag ttgatgctgg cgtagggggc gcggccgttg tgctggtaga
      601 ggtcgctgga cccgctgagg cggtaggcaa gttcggccac ctgcgactcg tcgccgcgcc
      661 agaagctgcg caccgtgtcg cggtacttgc cgttccactc cgaccagttc accgggaagt
      721 tgcccacctg gtagccgccc tccccgatgt cccacggctc cgcgatgagc ttcacctggg
      781 agatcaccgg gtcctggtgg attatgtcaa ggaagctgtt cagcctgccc acttcgtgca
      841 gaccgcgcgc caacgtggag gcgaggtcga accggaagcc atccacgtgc atctcggtta
      901 cccagtagcg caggctgtcc atgaccgttt ggagggtgcg cgggtggagc atgttgaggg
      961 tgttgccggt gccggtgtag tccatgtaaa agcgggggtc gtcgggcacg aggcgatagt
     1021 atgacacgtt gtcgatgcac ttgaagctaa ggtgcgggcc gaagtggttg ccctcggcgg
     1081 tgtggttgta gaccacatcg aggatgacct caatccccgc cgcgtgcagc gtcttgacca
     1141 tcgccttgaa ctcgcgcacc tgctcgccca gcttgccgga actggagtac tccgagtagg
     1201 gggcgaagaa gccgatgctg ttgtaacccc agtagttgct cagccccttg tccaccagcg
     1261 tcttgtcgtt gacgaagtgg tgcaccggca gcagctccac ggcggttatg ccgagggtcc
     1321 ggaagtagtc gatagcctcg ggcgtggcaa gcccggcgta ggtaccccgc agtttagggt
     1381 cgatgtcggg gtgctgggcg gaaaagccct tgacgtgcac ctcatataag atagattcgt
     1441 gcagcggggt gttgggggct ttgtcgtcgc cccaatcgaa cgccgggtct atcaccacgg
     1501 agcggttcat gccgggcgcg ctgtcctggg tgtccatctg gaggtcgcgg tcggggtctt
     1561 cgaggcccat cgggtagccg aatagggcgt cgctccagtc gatgcggccc gttatggcct
     1621 tggcgtaggg atcgagcagg agcttcgccg ggttgaagcg gtgcccctgg gaaggctcgt
     1681 aggggccgta cacgcggtag ccgtatagct gtcccggctt gaggttgggc atgtagacgt
     1741 gccagacata gtcggtctgc tcggtcatgc ggatgcgcat gcgctcggcc tcgtcgtcgg
     1801 tcgagtcgaa caggcagagt tccacgccgg tggcattctc gctgaagagg gcgaagttta
     1861 cgcccgtgcc gtcccaggta gcgcccagag ggtacggctt acccggccat acgttcatgt
     1921 cgtccatgct ccttgttcta acgcttcttg ttgttgtgac caactatgca agtccccctc
     1981 aggtaacaat tccttctttt attttcgttt caccgtgcag ggacgggcac gatgggccag
     2041 cccctacaga acgaccgcat tgggaaatag aacgtcctaa accttctcca gcagggccac
     2101 cgggaacacc gccagcactt cctcaagcgg cagccctacg cttccgttcc gcgaggctgc
     2161 cgaaagggtc tccccggtga agacgttgcg gaacgagtcg ccgtccaacg cgccgggcag
     2221 cggcagccac gaaccggacc acaccggccc taccggcacc acggtctccc ctgccttgcc
     2281 gagctgggtc accaggcaag gcgctaccgt cgccgccagg ctgccctcgt aggcgcgagc
     2341 gtaggcgacg atgttacccg cgtgcttgcc ctctaccgcg atgggtgtgt aagagccatg
     2401 ctggaagagt cgcaggttag cacgcctgaa gtttagggcg cggctggtca cgagcagctt
     2461 gatgcgtccg tcctccttgg tattgacgag cgacgcgacg gcacctactt ccttcacctc
     2521 ctccacctgg tcgagcaggc ggcggcgcag gccgtaatcg atagggcggc ggttgtcggg
     2581 gtccaccaag ctgaagtccc agacctcgtt gccctggtag atgtcgggca cgccggggct
     2641 ggtgagcttg atgagcacct gcgacaacga gttgaatgcg ccgtagtggg ccacgcgccg
     2701 ctggaacggc aggaaatcag cgaagaagcg gttgtcctcg tctcggcgca gtattgtcgc
     2761 gatgaagtcg gccacagcct gctggtacgc ctcgttgggg ttgacccagc ttgtgttcac
     2821 cttggcctcg ttcatcgcct tgcgcatgta agcctgcacc cgctccacca cctcggcgtg
     2881 cgcttcttcg cccaggtctt cttcgagggg ccacacgcct accaacgtct ggtagaggaa
     2941 gtactcctcg ttggggctgg gggcgggctg gccctcgacg ttcttcttgt gcttgcggtt
     3001 ggccctggcc cagcggcgga aggcggtgcg ccactccttg ggcatctccg acagcacgtc
     3061 tatgcgcatc cgcacgtctt cgctgcgctt ggtgtcgtgg gtactggtgg tgagcaggga
     3121 gaagggccaa tgccgctgcc gctcggcctg cgccttgtgg aacgcggcgg ggtaaatgcc
     3181 gaagtagccc ggctcgccgc ccacctcgtt gagcgagatg aggcggttgt acacgtagaa
     3241 ggcggtgtcc tccaggccct tggcgatgac ggggccggtc acctgctgga acttcatcac
     3301 gaagttggta cgctcggcgc actcctccgg cggcaggtca tcgaagcccc tgagcatcag
     3361 gatgtcgccc aggaagtcaa acgcgctggg gtccacggcg gggttgcgct tgcgggcgag
     3421 ggctacggcg cgctcgatgt gcgcctggtc acgccgctct acctcggtgg tctctggcgt
     3481 gatgtaggtg cggtagacgg ggaagcaggc tatcacctcg cgaatggcat tgcggatgcc
     3541 gttgagggtg aagtcgcggt aatggcggtc gcgctcggat atgcggttga gcatgttggt
     3601 gagcacgttt atctcgctgg aaagggacag gcgcatgatg cgctgcttgc tctcgtacac
     3661 caggtccgcg aacttcacct tctcgcccat gatggagttg taaatctcat cgaacgccct
     3721 ggcgttggcg ctgtccacga agaggccgtt ggcgtcgttg gtgaactcgt agccggtggt
     3781 gccgtgcacc acccaatcgg cgggcagggc ctcgccccgc gccagtatct tctcgaccac
     3841 cacgtagagc ggcctgttga gcacgccctg tgggtccgcc attcgctcgg catcgaaccg
     3901 gcccaccagc agggcctcaa gctcggcgcg gtcggtcctc tcaagctccg ggcctagctc
     3961 gtccatgacc aggcggcaaa tctccaggaa gtagctgtgc tgcaagtcct ggaagtaccg
     4021 ggcggggtcg tagaggccgt caacgtggtc gatgcgcagg ccgtggagct tcccttcgcg
     4081 gaggaagcgc ataatcatgc ggtgcgtctc ctggaagacg gggggctggt cgatgcgcac
     4141 ggcggcgagg tcgttcacgt caaagaagcg ccggtagttg atctcctcgg tcgccacgcg
     4201 ccagaagctg aggcggtagg gttggcgttc cagcagggag tcgagcaggt cgaagctatg
     4261 cggctcgccc tgcacgccgt tgaagcgttc tacgttgccc tcgatgaacg cgctcacgcg
     4321 cggctccgaa gcgcagaggg aggcgatgcg gcgcttgatg acctcttttt cgcggttgcg
     4381 ctcaagtatc ctctcccgct cggtctcgaa gtgggagggc aggtggtcca ggtcggcttc
     4441 ggtgcctatg gaggggttta gctcgttgtg gttggcgatg tcgtagccgt gcaggctttc
     4501 ggcgcgggcc ttgaagtagg gcgagg
//
LOCUS       DASRWZ010000108         1997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_968543, whole genome shotgun sequence.
ACCESSION   DASRWZ010000108 DASRWZ010000000
VERSION     DASRWZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968543"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1171
                     /locus_tag="VFH60_01570"
     CDS             329..1171
                     /locus_tag="VFH60_01570"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6312497.1"
                     /translation="METPKIQIDNLRVVYPGRGATGPVEALGGVTLHVPEGELICVVG
                     PSGCGKTTLLRVLAGLEKPATGTATIRHDDAARPAMAMVFQGAGIFPWLTVEDNVAYG
                     LQLRGVDREERLKTARHWIGEIGLERFARSYPAQLSGGMRQRVGLARALAVDAEVLLM
                     DEPFGALDAQTRLILQQTLLEQWERNAKTVIFVTHSIEEALTLGDRVYVMSARPGLLL
                     KEFRVPFERPRDAVALRTDPRFSTLFAGVWEVLRDEVDRARLAELGTVSDTGLESTKV
                     EVPM"
     gene            1168..>1997
                     /locus_tag="VFH60_01575"
     CDS             1168..>1997
                     /locus_tag="VFH60_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6312498.1"
                     /translation="MRSSVASNRWLSLLSPLLLVALWELAVQVGWLNRVFVPPPSEVF
                     VALGQLIQSGQLFRDLGVSLVRVGLGFLYGGVPAIALGLLMGISPLVRAIVRPIATAI
                     YPIPKIALLPLVIVVLGIGEASKVATIAVSVFFLVVLNVAASVMQIDAQYFEVARTFG
                     AKSKDLFWTVALPASLPGIMNSVKIGMGFTLTLIVGVEFVGANEGIGYLIWRSYELYA
                     INRMLAGLVAVAVVGWLITVLLDEIEYALVPWQATSVKLKEARMRQRAQVWFKASRPW
                     "
BASE COUNT          336 a          597 c          691 g          373 t
ORIGIN      
        1 tcgacgcgga gaagtgacga gttgctagtt gttagtgaag agtggcgagg ttaggggaaa
       61 gaaggggacg tggatgccac atttttggcg aagcgcgacc aacgctcccc tctgtcatcc
      121 tgagcgtagc gaaggatctc aagaagttgg agagggctgc tgttgaacgt tggcacaatg
      181 gtgctgaagg ttcagcacag tggtaggggc gacatcttag atccttcgct acgctcagga
      241 tgacaggggc actcttagtc ctgctacact aacaaatcgc attcacgtac tgacagtcgc
      301 aagccgcaca ataccgctca tttcagctat ggaaactccg aagatacaaa ttgataacct
      361 gcgagtggtc taccctgggc ggggagctac cgggccggtg gaggcgcttg ggggcgtgac
      421 gctgcacgtg ccggaggggg agcttatctg cgtggtgggg ccttccgggt gcggcaagac
      481 caccctcctg cgcgtgctgg cggggcttga gaagccggcc accggcaccg ccaccatccg
      541 ccacgacgac gcggcccgcc ccgctatggc gatggtgttc cagggcgcgg gcattttccc
      601 ctggctcacg gtagaggata acgtggccta cgggctgcaa cttcggggag tggacaggga
      661 agagcggctg aagacggccc ggcactggat tggcgagata gggctggagc ggttcgcccg
      721 ctcgtacccc gcgcagcttt cgggcggcat gcggcagcgt gtcggcctcg cccgtgccct
      781 cgcggtggac gccgaagtgc tgctcatgga cgagccgttc ggcgcgctcg acgctcagac
      841 ccgccttatt ttgcagcaga ccctcctcga acagtgggag cgcaacgcca agacggtcat
      901 cttcgtaacg cacagcatcg aggaggccct cacgctgggc gaccgcgtgt acgtcatgtc
      961 ggcccgcccc ggtctgctac tcaaagagtt ccgcgtgccc ttcgagcggc cccgcgacgc
     1021 cgtggccctg cgcaccgacc cccgcttcag caccctcttt gccggcgtgt gggaggtgct
     1081 gcgcgacgag gtggaccggg cgcggctggc cgagctgggc accgtgagcg atacggggct
     1141 ggagagcacg aaagtggagg tgccgatgtg agaagcagcg tggcgagcaa ccgctggctg
     1201 tcgctcctgt cgccccttct gctggtcgcg ctgtgggagc ttgccgtgca ggtgggctgg
     1261 ctgaaccgcg tgttcgtgcc gcccccctcc gaggtgttcg tggcgctggg ccagctaatc
     1321 cagagcgggc agcttttccg cgacctgggc gtcagcctgg tgcgcgtggg cctcggcttc
     1381 ctgtacggcg gcgtaccggc catcgcgctg ggcctgctca tgggcatcag ccccctcgtg
     1441 cgcgccatcg tgcgccccat cgcgaccgcc atctacccca tccccaagat agccctgctt
     1501 ccgttggtaa tcgtggtgct gggcatcggg gaggcgagca aggtagccac gattgcggtg
     1561 agcgtattct tcctggtggt gctcaacgtg gccgcctcgg tgatgcagat agacgcccag
     1621 tacttcgagg tggcccgcac cttcggggcg aagagcaagg acctgttctg gacagtggcc
     1681 ctgcccgcca gcctgcccgg catcatgaac agcgtcaaga taggcatggg cttcaccctc
     1741 acgttgatcg tgggcgtgga gttcgtgggc gcgaacgagg gtatcggcta cctcatttgg
     1801 cgctcctacg agctatacgc cataaaccgc atgctggcgg ggttggtggc tgtggccgta
     1861 gtgggttggc tcatcacggt gctgctggac gagatcgagt acgcgttggt gccgtggcag
     1921 gcgaccagcg tgaagttgaa ggaggcgcgt atgcgacagc gcgctcaagt gtggttcaaa
     1981 gccagtcgcc cctggag
//
LOCUS       DASRWZ010000109         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_146684, whole genome shotgun sequence.
ACCESSION   DASRWZ010000109 DASRWZ010000000
VERSION     DASRWZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146684"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFH60_01580"
     CDS             <1..504
                     /locus_tag="VFH60_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312499.1"
                     /translation="MLSILPVPGTAPLPFPVDRFSIVAVVAASLLFDLIASKEPPQDE
                     LWPSLAILLALMSPLAWQGELMPGNGDEGVSGYLAGAATLVGLAGLFGGTRYGWSAAA
                     RWLAWWGSALALGTLPAGAWGLVTLPAAMVLGWAAQRPGWGKYATLLACVLALGALAT
                     ALPRLPG"
     gene            complement(476..1618)
                     /locus_tag="VFH60_01585"
     CDS             complement(476..1618)
                     /locus_tag="VFH60_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET6312500.1"
                     /translation="MPTVDDIETYFLCQGCQKSEEVAQRLADFFRTARKSIDIAIYSF
                     SLCPEPRRILYAALHEQVKAGVQVRIVYDEKAGKDSTFGPLGDYCAFNTPDFVKTLDL
                     PIRGATGQRALMHHKYVILDAGTPEARVWTGSLNWTDDGWEKQENNVIIVRSQELAAY
                     YLHDFEELWVDGNLAPSGTMDSGEDTVQYKGEPAHVLLNFSPGEGEWIDEAIANQIDR
                     THRRATLAFVVLTSSRILRAVQGLMMRNVPIVGIYDESQMEGVKYQWSLVPANHWKIP
                     AFKEIIQYAQLVGKRSTPYTENSQHDFMHNKIMVLDDVVVTGSYNFSRHAQQNAENSL
                     IITSAALAETYNTYLEGLMDKYRQTGPTKPPPASKELVSPEAAEAQ"
     gene            complement(1602..>2033)
                     /locus_tag="VFH60_01590"
     CDS             complement(1602..>2033)
                     /locus_tag="VFH60_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312501.1"
                     /translation="DGQVECSVRGSGRGAISRRFRGCCARKGCFALDVVQVFLVTFGV
                     LVAIGGLVLMLRSARRGDDGRRGYLPFAMLIVGLIVAYRSFSDFRTLDAQDITILFLF
                     AIALLTLIGLQFFVMERSGSYLDAPAEPEGRKGEGEDANSG"
BASE COUNT          339 a          634 c          660 g          400 t
ORIGIN      
        1 atgctgtcca tcctgccggt gcccggtacg gcgcccctac ccttcccggt agaccgcttc
       61 tccatcgtgg cggttgtagc ggcctccctg ctgttcgacc tgatagcctc gaaagagccc
      121 ccccaggacg agctatggcc cagcctggcg attctcctcg cgctgatgtc gcccctcgcc
      181 tggcaaggag agcttatgcc gggcaacggc gacgagggtg ttagcggcta cctggcgggg
      241 gcggccacgc tcgttgggct ggcggggctg ttcggcggca cgcgctacgg gtggagcgcg
      301 gcggcccggt ggctggcatg gtgggggtcg gccctggcgt tggggacgct gcccgccggt
      361 gcgtgggggt tggttacact accggccgcg atggtgctgg gttgggccgc gcaaaggcca
      421 ggctggggca aatacgccac tctgctggcc tgcgtactgg cgctgggggc gctggctact
      481 gcgcttccgc ggcttccggg ctgaccagtt ctttcgaggc gggcgggggc ttagtggggc
      541 cggtctgccg gtacttgtcc atgaggccct cgagataagt attgtaagtc tcggccagcg
      601 cggcggacgt gatgatgagg gagttttcgg cgttttgctg ggcgtggcgc gagaagttgt
      661 acgagccggt aaccaccacg tcatcgagca ccatgatctt gttgtgcatg aaatcgtgct
      721 ggctgttctc cgtgtagggg gtgctacgct tacctaccag ttgggcgtac tgtatgatct
      781 ccttaaaggc cgggatcttc cagtggttgg cgggcaccag gctccactgg tacttcacgc
      841 cctccatctg cgactcgtcg tagataccta caatcggcac gttgcgcatc atgaggccct
      901 gcaccgccct gagaattcgg ctggaggtta gcaccacgaa ggccagcgtc gccctgcgat
      961 gggtgcggtc tatctggttg gcaatcgcct cgtcaatcca ctcgccctcg ccgggagaga
     1021 agttgagcag cacgtgcgcc ggctccccct tgtactgcac ggtgtcctcg ccgctgtcca
     1081 tcgtgccgga tggtgccagg ttgccgtcca cccaaagctc ctcgaagtcg tgcaggtagt
     1141 aggcggcaag ctcctgcgag cgcacgatga tgacgttgtt ctcctgcttc tcccagccgt
     1201 cgtcggtcca gttgagcgag ccggtccaca ctcgggcctc gggcgtaccc gcgtcgagga
     1261 tgacgtactt gtggtgcatc agggcacgct gccctgtcgc gccccttatg gggaggtcca
     1321 gggtcttcac gaagtcgggc gtgttgaagg cgcaatagtc gcccagcggg ccgaaggtgc
     1381 tatccttgcc cgccttttcg tcgtagacga tgcgcacctg cacgcccgcc tttacctgct
     1441 cgtgcagggc cgcatagagt atccggcgcg gttccgggca gagcgaaaac gaatagatag
     1501 cgatgtcgat agacttgcgc gcggtgcgga agaagtcggc caggcgctgg gccacctcct
     1561 cagacttctg gcagccctgg cacaggaaat acgtctctat atcatccact gttggcatct
     1621 tctccctcac ctttgcggcc ctcgggttcg gcgggcgcat caaggtaaga cccgctacgt
     1681 tccattacga agaattgcag ccctatgagc gtcagcagag ctatcgcgaa caagaacaga
     1741 atggttatgt cctgcgcgtc gagcgtgcgg aagtcgctga aggagcggta ggcgacgatc
     1801 aggccgacga tgagcatggc aaacggcagg tagccccggc ggccatcgtc tcccctccgc
     1861 gcgctccgca gcatgaggac caggccgcca atagcgacca gcacaccaaa cgttaccagg
     1921 aagacttgca cgacatccaa agcgaaacag cccttccgcg cgcaacaacc acggaagcgg
     1981 cgagagatag ccccccggcc gcttccgcga acgctgcact ctacttgccc gtc
//
LOCUS       DASRWZ010000110         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1311491, whole genome shotgun sequence.
ACCESSION   DASRWZ010000110 DASRWZ010000000
VERSION     DASRWZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311491"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1200)
                     /locus_tag="VFH60_01595"
     CDS             complement(55..1200)
                     /locus_tag="VFH60_01595"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET6312502.1"
                     /translation="MSATRPTPHTNSDPDLGFEVEIKTSGRAYDHGQPSSPAAPATVS
                     PTRAAAKAAARRRNLTLLILGIAALAIAGLWAFLSNPNFNVPSDAVARVNGEYIYNGE
                     VTREIDLARATFELAKSDEQPQSPGQVLETLIDRKLQTQDARRNGVAVSQAEVDAALK
                     GVLDRLNVTEEEMQSTLEKYNLTLDDLRVPVADAYLINKYIGTCIVSGTTSEQEVLSR
                     KNDWQTGLAQNSRIEYLKSPGAGPAPTINSEAPDFTLRNLEGEEVKLSDLRGKPVMVN
                     FWATWCAPCRVEIPEIVKMYRETHAGGNYEILGVATSSSLETVRAFSQEFDMQFPLLP
                     DDGNHITNLFHVLPIPTTFFIDKDGIIRETQIGPVDRALMEKWLLPK"
     gene            complement(1202..2008)
                     /locus_tag="VFH60_01600"
     CDS             complement(1202..2008)
                     /locus_tag="VFH60_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET6312503.1"
                     /translation="MALSFFAGLLSFLSPCVLPLVPAYIGYLGGSALPVARGVAAAGT
                     VGAAGAVGATGGQAPLTQATARWIVMANAFLFVLGFTIVFAMIGNLAGALSDLLYENK
                     RLLQYIAGGMLFIFGLHMIGLIRIQFLDYTRRLDVRPAQNLGYVRSFLIGLGFGIGWT
                     PCIGPTLGLIFTLALNGKTSDAFVPFLAYSLGLGVPFLATALAMGQISGVLKRLTRRS
                     YTLKAGKWTIIDQVNIVSLVSGVLLVVMGFLVFFNLVTLLAPPDVNAFVI"
     gene            complement(2076..>2317)
                     /locus_tag="VFH60_01605"
     CDS             complement(2076..>2317)
                     /locus_tag="VFH60_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312504.1"
                     /translation="LRDTMLDMTVEANFGMFTIGSQPLSLNWWLRIITGFLAACGTVW
                     FAFPRMDRAVAESEAQAQIYRMSALNYPPGMAQRV"
BASE COUNT          422 a          707 c          760 g          428 t
ORIGIN      
        1 atcgttccgt gtcctttgcg ctctttgcgc ctttgcggta tatccgtaaa attgttactt
       61 cggaaggagc catttctcca tcagggcgcg gtctacgggg ccgatctggg tttcgcggat
      121 aatgccgtct ttgtcgatga agaaagtggt ggggatgggg agtacgtgga agaggttggt
      181 gatgtggttg ccgtcgtcgg gcaggagggg gaactgcatg tcgaactcct gcgagaaggc
      241 cctgaccgtt tcgagactgc tgctggtcgc cacgccgagt atctcgtagt tgccgcccgc
      301 gtgggtctcc cggtacatct tgactatctc gggtatctcg acgcggcagg gcgcgcacca
      361 ggtagcccag aagttgacca tgaccggctt gccgcgcagg tcgctcagtt tgacctcctc
      421 accttcgagg ttgcgcaacg tgaagtcggg ggcctccgag ttgatggtgg gcgcggggcc
      481 tgcccccggc gacttgaggt actctatcct gctgttctgg gccaggccag tctgccaatc
      541 gttcttgcgg ctcagcacct cctgctccga ggtcgtccct gacacgatac acgtaccgat
      601 gtacttgttg atgaggtagg cgtccgcgac cggcacgcgc aggtcgtcca gcgtgaggtt
      661 atacttctcc agcgtggact gcatctcctc ctcggtcacg ttcagcctgt ccagcacgcc
      721 tttgagggcc gcatccacct ccgcctggga gacggccacc ccgttgcgcc gggcgtcctg
      781 cgtttgcagc ttgcggtcga tgagggtctc gagcacctgg ccggggcttt gcggctgctc
      841 gtcgctctta gccagctcga acgtggcccg cgccaggtct atctcccgcg tcacctcgcc
      901 gttgtagata tactcgccgt tcacgcgggc cactgcgtcg gacggcacat tgaagttggg
      961 gttggacagg aaagcccaca gccccgcaat cgccagcgcg gcaatcccca ggataagcag
     1021 cgtcaggttg cggcggcggg ccgccgcctt ggcggcggcg cgggtgggcg agacggtcgc
     1081 aggagcagcc ggagatgagg gctgcccgtg gtcgtaggcg cgcccgctgg tcttgatttc
     1141 tacctcgaag cccaggtcgg ggtcggagtt ggtgtgaggc gtgggtctgg tagcgctcat
     1201 attagattac aaacgcgttg acgtcgggag gggccaggag ggttaccagg ttgaagaata
     1261 cgaggaagcc catcaccacc agcaggacgc cgctcaccag cgagacgatg ttcacctggt
     1321 ctatgatggt ccatttgccc gccttgagcg tgtagctgcg gcgagtgagg cgcttcaaca
     1381 cacccgaaat ctgccccatc gccagcgccg tcgcaaggaa gggcacgcca agccccagcg
     1441 agtaggcgag gaagggcacg aaggcgtcgc tggtcttgcc gttgagggcc agcgtgaaaa
     1501 tgagaccgag agtggggccg atgcaagggg tccagccgat gccgaagccc aggccgatga
     1561 ggaaggagcg cacgtagccg aggttctgcg cgggccgcac gtcgaggcgg cgggtgtagt
     1621 cgaggaactg tatgcggatc aggccgatca tgtgcagccc gaagatgaac agcatcccgc
     1681 cggctatgta ctggaggagg cgcttgttct cgtaaagcag gtcgctcagc gccccggcaa
     1741 ggttgcctat catggcgaac acaatcgtga agccgagcac gaacaggaac gcgttagcca
     1801 tgacgatcca gcgcgccgtg gcctgggtca aaggtgcctg gccgcccgtc gcgcctactg
     1861 cgcctgccgc gcccactgta cccgctgccg ccacaccccg cgccacgggg agggccgacc
     1921 cgcccaggta gcctatgtaa gccggcacca acggcagcac gcacggcgac aggaacgata
     1981 acagccccgc gaaaaacgat agcgccaaaa aagtaagcgt ctccatacag tttccctcta
     2041 agtagttaca acgtctgcaa cgcggcctct gcctctcaca cccgctgcgc catgccgggc
     2101 gggtagttca gggctgacat ccggtagatt tgcgcctgcg cctccgactc ggccaccgcg
     2161 cggtccatcc tggggaaggc gaaccagacg gtcccgcacg cggccaggaa cccggtgatg
     2221 atacgcagcc accagttgag agagagcggc tgcgagccta tcgtgaacat gccgaaattg
     2281 gcttccaccg tcatgtcgag catagtatcg cgcaggc
//
LOCUS       DASRWZ010000111         3318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1037424, whole genome shotgun sequence.
ACCESSION   DASRWZ010000111 DASRWZ010000000
VERSION     DASRWZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037424"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFH60_01610"
     CDS             <1..241
                     /locus_tag="VFH60_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312505.1"
                     /translation="FIFVESRPGFDVEEIAPRRNLVMMHTPGGAEGAPRAAADAAIEA
                     SGRPHEEVVTGYHDEVHRFVRRPFVLVPTSPDLEE"
     gene            322..1437
                     /locus_tag="VFH60_01615"
     CDS             322..1437
                     /locus_tag="VFH60_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016471748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET6312506.1"
                     /translation="MYDVAVVGGGPAGAVAALGLARASRRVLLLDKSGAGEFKVGEGL
                     PPAAMPLLRDLGLREAFLAGGHLPSYGNLSAWGSPHLQSTDFIFDPNGHGWHLDRARF
                     DAFLREHAREAGAEVRGATSLRRHEWDEEAGLWRLSLLGREGKAEAQCRWLVDASGRR
                     CVVARREGARRRADDALTAVYALFGQADDGAGGDQDSRTLIEAAPDGWWYTALLPSNR
                     RVAVYHSDTDLLPTSTLLAPEGFRSLLDGTEHVRGYFAGPGYTMLGLPRATSAQSARL
                     GWFVGAGWLAVGDAALSFDPLSSQGIMTALFAGMVAGVALDAHLSGEPDALDSYSARL
                     AAIYRAYLNNRLFYYGYESRWADRLFWRRRKAGAARD"
     gene            complement(1704..2861)
                     /locus_tag="VFH60_01620"
     CDS             complement(1704..2861)
                     /locus_tag="VFH60_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET6312507.1"
                     /translation="MAQLVKQQQDEARQNGKDGASPPLEGQEAYAPEEAHDNIPPGTR
                     VAVIINPAAGKKGGITTNASGVDDVRRVLEANGIQADFLETQYPQHATELAQQALKDK
                     YDIVIACGGDGTVGEVATALIGRKITLGILPLGSANNVARMMHVPFDLEEAAKVLRLG
                     EIKKVDAGRCNGTYFLETAGVGLDAAIFPILNKVDKGEYVRLFDALNTFFTFRPRSVT
                     VVLDGRAIRIKALVVLVANGPYWGYSVPLAPEARVDDRRFDVVIFRNFSKWSFVRHVL
                     RAMLHRADPRRLVGHGQHKQNGKQITMANSAGHPDVRVYRAKKVQVLTSERRPWPVHA
                     DALPRGHTPAQIDLIPGALRVITGPGEHVTDPPSKGTPKGTPPEHRLRSET"
     gene            3098..3316
                     /locus_tag="VFH60_01625"
     CDS             3098..3316
                     /locus_tag="VFH60_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HET6312508.1"
                     /translation="MKEDEREDTTIYKVVVNFEEQYSIWPADRKDPMGWRDVGKHGTK
                     AEYFAYLEKVWRDMGPHDLRIQTDEAGH"
BASE COUNT          555 a          940 c         1186 g          637 t
ORIGIN      
        1 cttcatcttc gtggagtcgc ggccggggtt cgacgtggaa gaaatcgcgc cacggcgcaa
       61 cctggtcatg atgcacacgc cgggaggcgc tgaaggcgcg ccacgcgcag cggccgatgc
      121 ggccatcgag gccagcggga ggccccacga ggaggtcgtg accgggtacc acgacgaggt
      181 ccaccggttc gtgcgccggc ccttcgtgct tgtgcccacc agcccggacc tggaagagta
      241 agggggagtt ggaatttcgg atttcgaatt tgttggattc ctactaaact cgaaattcca
      301 aattccaaat tcgtaattcg tatgtacgac gtagctgtgg tggggggcgg gcctgcgggg
      361 gcggtggcgg cgctggggct ggcgcgtgcg tctcggcggg tgttgttgct cgacaagagc
      421 ggggctgggg agttcaaggt tggcgagggg ttgccgcccg ccgcgatgcc gctgttgcgc
      481 gacctggggc tgcgggaggc tttccttgcc ggggggcacc tgccgtcgta tggcaacctc
      541 tcggcctggg gctcgccgca cctccagagc accgatttta tcttcgaccc gaacgggcat
      601 ggttggcacc tggaccgcgc gcgttttgac gcatttttgc gcgagcacgc gagggaggcc
      661 ggggccgagg tccggggtgc tacctcgctg cggcggcacg agtgggacga ggaggcggga
      721 ctctggcggc ttagcctgct ggggcgtgaa ggtaaggcgg aggcgcagtg ccgctggctg
      781 gttgacgcga gcgggcgtcg ttgcgtcgtg gcgaggcggg aaggtgcgcg ccgcagggcc
      841 gacgatgcgc tgacggccgt gtacgccctg ttcgggcagg cggacgacgg ggcaggcggg
      901 gaccaggact ctcgcacctt gatcgaagcg gcccccgacg gctggtggta cacggcgctg
      961 ctgccctcta accgccgcgt cgccgtctat cacagcgaca ccgacctgct gcctacctcg
     1021 acgctgctcg cgccggaggg cttcaggtcg ctgctggacg ggacggagca cgtgcgcggc
     1081 tacttcgcgg ggcctggcta cacgatgctg gggcttccca gggctacttc ggcgcagagc
     1141 gcccgcctgg gatggttcgt gggggcaggc tggctcgcgg tgggggacgc cgcgctctct
     1201 ttcgacccgc tctcctcgca gggcatcatg acggcccttt tcgcgggtat ggtggccggg
     1261 gtggcgttgg acgcgcacct ctccggcgag cctgacgccc tcgacagcta cagcgctcgc
     1321 ctggccgcga tttacagggc ctacctcaac aaccgcctgt tctattatgg gtacgaaagc
     1381 cgctgggcgg accgtctctt ctggcggcgg cgcaaagctg gggcggcgcg cgattaggct
     1441 ctatctgtac agagtaggaa gcaagcgaac agaggtggcc cctgtcattc tgagcgcaga
     1501 gaagaatcta agatgctact tgtgccccgt tgtcgaacgt cagcagcatg gtacctatgg
     1561 ttcaacaacc tccctgtacc aacttcctta gattcttcgc tacgctcaga atgacagggg
     1621 ccactctggt cccgcgtagg cggcactttg cagatgggcc ctagcctgac agtcacttca
     1681 cgcagcgtgt agagcaagcg tggttacgtt tcggagcgga gccggtgctc agggggcgtg
     1741 cctttggggg tgcccttgga aggcgggtcg gtgacgtgct cgccggggcc ggtgatgaca
     1801 cgcagggcac ctgggatgag gtcgatctgg gcgggggtgt ggccgcgtgg gagggcgtcg
     1861 gcgtgcacgg gccaggggcg gcgctcggaa gtaagcacct gcaccttctt ggcgcggtag
     1921 acgcgaacgt cggggtggcc agcgctattt gccatggtga tctgtttgcc gttttgcttg
     1981 tgctggccgt gtcccacgag gcgacgaggg tcggcacggt gcagcatcgc ccgcaggacg
     2041 tggcggacga aggaccactt gctgaagttg cgaaagataa ccacgtcgaa ccggcggtcg
     2101 tccaccctgg cttcgggggc gagcggcacc gagtagcccc agtaggggcc gttggcgacg
     2161 aggaccacca gggcctttat gcgtatggcg cgcccgtcga gcaccacggt gacggagcgg
     2221 ggcctgaagg tgaaaaaggt gttgagggcg tcgaacaggc ggacgtattc gcccttgtcc
     2281 accttgttga ggatggggaa gatagcggca tcgaggccga caccggccgt ttcgaggaag
     2341 taggtgccgt tgcagcgacc ggcgtctacc ttcttgatct caccgaggcg gagcactttg
     2401 gcggcctctt caaggtcaaa cggcacgtgc atcatgcggg ccacgttgtt ggcgctgccg
     2461 aggggtagga tgccgagggt gatcttgcgg cctatcaggg cggtcgccac ctcgcccacc
     2521 gtgccgtcgc cgccgcaggc aatcacgatg tcgtacttat ctttgagggc ctgttgggca
     2581 agctcggtgg cgtgctgggg gtactgggtt tcaaggaagt cggcctggat gccgttggct
     2641 tcgagcacgc ggcgcacgtc gtccacaccg gaggcattag tggtgatgcc gccctttttg
     2701 cccgcggcgg ggttgatgat tacggctacc ctggtgcccg gtggtatgtt atcgtgggcc
     2761 tcttcgggtg cgtaggcttc ctggccctcc agtgggggtg aggcaccgtc tttgccgttc
     2821 tgtcgcgctt cgtcctgttg ctgttttacc aattgggcca ttcttacgtc ctctttcccg
     2881 cgaacaagta gatgcgaagt atcacaagcg actgtaatgc aaatgccggg ccaagggtca
     2941 agggagcagc gatgtcttga cggccttcct ctcgattcgg acaggaaggg cctggagcct
     3001 ggtggtatac tctatccaca atccgtatct ggacgcacac cgccaggaca acgtaattag
     3061 accaatcaca aggcacgcca ggaaatcagg aggcagcatg aaagaggacg aacgggaaga
     3121 cactacgatc tacaaagtgg ttgtgaactt tgaagagcag tattccatct ggcctgccga
     3181 ccggaaggac ccaatgggct ggcgcgacgt gggcaagcat gggaccaagg cggagtattt
     3241 cgcctatctt gagaaggtgt ggagggacat ggggccgcac gacttgcgca ttcaaacgga
     3301 cgaggccggt cattaacg
//
LOCUS       DASRWZ010000112        11030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_626378, whole genome shotgun sequence.
ACCESSION   DASRWZ010000112 DASRWZ010000000
VERSION     DASRWZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626378"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..1657
                     /locus_tag="VFH60_01630"
     CDS             296..1657
                     /locus_tag="VFH60_01630"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6312509.1"
                     /translation="MQPNNPSIPGPDTRPARVLMYCHDGFGLGHLRRTTTLAQNLRDA
                     YPNSGILLAVGSASLPFWKDLPGLDYIKLPSIRKVATDCWSARSLPISADMAHGIRAS
                     VLREVMLRYQPDLFVADYLPLGPAGELVPALKAAKAIGTRCVLGLRDILDAPDTVARL
                     WAQHGIAEAIEEWYDRVLVYGDPGVFDSVSAYGFSAKIASMARYCGYVVNDSPHEAPE
                     KVRARLNLATSRLVLVTGGGGQDAAPLVRSYLRGLREHVQSHDAPSFDSLVVLGPLMD
                     RNTRNAIREEAQGLPGSIRTEVGDIRRYVAASDLVVTMGGYNSLMEVALARKPAVVVP
                     RHGPSQEQKMRAGLFASRGWLVAAPDSETITGRELLGIIERTLDDPPRGAWEGFRMEG
                     AHKSVEALADCLADRHAGRLVTLPEIGYSLPHGRLRKSYGPGRTPSIRPPYADGSPPP
                     QLQ"
     gene            1753..3012
                     /locus_tag="VFH60_01635"
     CDS             1753..3012
                     /locus_tag="VFH60_01635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010999319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6312510.1"
                     /translation="MSNTYENRPLRVGYVLKRYPRYSETFVVNEILAHEAAGVQIDIF
                     SLRPPNDDHFQDVISQVRAPVTYLPSYGLKAADFWQAIQDANGLLPGVCTGLEAARGE
                     DVRDVHQALMLAREVKLRGITHLHAHFASVATSVARLAAHFAGVPYTFTAHAKDIFHE
                     SVRKDDLRRKLEDAAGVVTVSGYNVDYLSEISGAARNKINRIYNGLHLGQFPYTSPQD
                     RAPLIVGVGRLVEKKGFCDLVYACALLARKGVDFRCLIIGTGDEEARLREQIERLGLT
                     DRVELMGPKPQCEVKQYVGSAAVFAAPCVVGSDGNRDGLPTVLLEAMALGTPCISTDV
                     TGIPEVLRDGKTGLMVPQHNPAKLAEAIERLLNDPQLRVNIARSARRLIEDEFGIDRN
                     SVRIRALFEQATAANVLEPVEAVGARA"
     gene            3015..4205
                     /locus_tag="VFH60_01640"
     CDS             3015..4205
                     /locus_tag="VFH60_01640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6312511.1"
                     /translation="MRVAYVCADAGIPVFGSKGASVHVQEVTGALLGRGARVTLFAAR
                     TGGEPPEGLEGVTVYDLPTPAQGERAERERAAFEANGMIREAISQAGPFDMVYERYSL
                     WSYAGMEYAREHGIPGLLEVNAPLIEEQAEHRGLVHRELAVEVADRAFGAATALLAVS
                     REVAAYLDALPSARGRVHVVPNGVNPGRFEGVEGARDRKEDGTFTVGFVGTLKPWHGL
                     PVLVEAFDLLCRRRHDARLLVVGEGPEREMMQAELERRGLLRHTRFTGAVAPHEVPQL
                     LADMDVAVAPYPARADFYFSPLKVYEYMAAGLPVVASRIGQLEDVIEHEANGLLCAPG
                     DPIALAGALDRLRQDAALRARLGQEARRSVLRSHTWDSVAARILEIAATRQALSGVME
                     RVGP"
     gene            4208..7330
                     /locus_tag="VFH60_01645"
     CDS             4208..7330
                     /locus_tag="VFH60_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET6312512.1"
                     /translation="MSRAMTLKETIPGFWRIIRRFWPYLRKQRKLMAGSLVALFAEVG
                     LRLLEPWPLKFVFDRVIPQQPGVTSSGIPLLDSLDVRMLLLVSAVGIVLITAMRALAA
                     YFNTVGFAMLGTRVLSQTRSDVYNHIQRLSLSYHTKARSGDLVVRIISDVGQLKDVAV
                     TALLPMLANVLILVGMLALMLLLQWQLTLLALAVAPLFALATVRISRRIREAARTQRK
                     REGAMAATASESISAIKVVQALSLEDTLAESFFSQNNKSLKQDVKTSKLAASLERTVD
                     VLIALATGLVLWYGANLVLDGALSAGELLVFLTYLKNGFKPVRDFAKYTGRLAKATAA
                     GERVMDVLEQEPDVRNMPGAVPAPALRGAVRFNNVAFEYEPGHPVLRDINLDVQPGQH
                     IALVGASGNGKSTLVSLIMRLYDPTEGRVLLDERDVREYTIDSVRSQISVVMQDTILF
                     AASVRDNIAYGSPEATEEEIEAAARLANAHDFIEAMPEGYDTMLGERGATLSNGQRQR
                     ISIARAAIRRAPIVILDEPTTGLDEENERTVMEALDRLARGRTTFLITHKLSQARKAE
                     TIVVLQDGSIVEQGTHAELMRAGGAYRKLYTGESLAGPGVEIAVPAASPAPAPAAVHM
                     VVGGEDTGDVSDTGSLQTSGGPVPETQLEGANKVKAQAYNSKETDRARKPRGWRRSFA
                     LMLGALAVTVALVAAAINANATGSATNGASTVAQASTGAPATSTVPSRRAVPTALADM
                     RSHVEATVAGGAVQATSVVTYSGYLTGVASLAWSPDNRTLALGSTDDVARLLDTQTGV
                     VSMLKGHTKDVYLVAWSPDGTRLATGGFDNRVRVWTAGGQLVSTHGDHSGIIAGLTWL
                     QDGRSLASVSTNMQIIQWDAESASQVFAVKGEGDFLYNVTWTPDGSIMAAGASDTVRL
                     WSREGKLLTVMSGHTDEIYCAAWSPDGKVLATGANDDTVRLWSREGKSLATLTGHTEF
                     INGVAWSPDGKRLASGAFDKTVRLWDVAGKQLGTLVAGAEVLAVAWSPDGKSLAAVLL
                     DGTVWVWKLA"
     gene            7344..9815
                     /locus_tag="VFH60_01650"
     CDS             7344..9815
                     /locus_tag="VFH60_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6312513.1"
                     /translation="MLSRYDAELVRRDTAIPGLATLLDAEAFAACLREHAPGADIRRV
                     RPFYVRYKPGMNCLVAYKVELPGGQVDIYAKGHGAGAADPLREAVEQPGIPGALGRGR
                     ISMRHLGIVVSVFPNDSKLEQLDRLGRPDEKTRLLRRVFSDRPELWEAEVRGIRYKPE
                     RRLVAQLMSGDKPQAVLKFYSDTGYADAKQSARVFNTRKADCRVLQVPRRLGHSDRHH
                     VLAFEWMEGQLLSEALAGADLDLAAIERVGVALAEGHRRPVGKLAPLNRESEASTLLS
                     VASGLAFLCPDLSEKVHGLARRLATYLMQQPPMHTPVHGDFYAKQVLLAGERVVILDF
                     DSAAAGDPAYDLGLFVAHLERGAIRHTVPQDKIHSVANALLSGYERESGQTFRERVMV
                     YAAAGLLKLAPHQFRNRERNWPEGMEAIIDRAEAVLSSVVAPAVIALAPGAQGEEPER
                     VGVQANGAVEAAIVAVSDPFGAVNDPAMPFLAQALNPADVHQQFRRQLSGFIGEGGRL
                     DLLGIRVVRHKMGRRCLVEYNVAVERPGEQPQVVALIGKARARGLDKATFETVSALWR
                     SGFGGDAEDGIGVPEPVGTVPKFQMWLHRKVPGVPATQLLAAKDGADLAGRIAEAAHK
                     LHSARIRPQKRHGMADELRILHERLPLLGQMRPEWAGRIERLLAACDRLGAALPRPRN
                     SGIHRDFYADQVMAHADRIYLLDFDLYCEGDPALDIGNFLGHIQEQALRTLGDPRVLA
                     HVEEALEERFVELSGEQTRRAIQVYTTLTLVRHIHISTRFPDRQQITEALFELCEQRL
                     ARPAALPNRRLGARPAQLVLQEGSV"
     gene            9823..10890
                     /locus_tag="VFH60_01655"
     CDS             9823..10890
                     /locus_tag="VFH60_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6312514.1"
                     /translation="MKARKSPLARGLLVALVAVMAAGCSQESLTGLRQGVATPIGGPA
                     ESPSQVPESSNTPGAVGEAPTRTPQPRASRTVPTGTPQLPPDNDDGEEAVLVGAGDIG
                     DCDSGDDERTAALLDEIEGTVFTLGDNVYDRGTAREFRECYEPSWGRHKERTRPSPGN
                     HDYRTDDAGPYFDYFGDSAGEKGKGYYTYDLGSWHIVVLNSNCKYVGGCEEDSEQLRW
                     LEDDLANNPARCTLAYWHHPLFNAGEHGNEKAVRPLWEALYDAGAEVVLAGHDHNYQR
                     FAPQDPGGKSEPERGIRQFIVGTGGKDHYDIEDSMPNLEASNDDANGALKLTLRAEGY
                     SWEFIPAEEDDFQDSGTGSCH"
BASE COUNT         1981 a         3493 c         3827 g         1729 t
ORIGIN      
        1 ctacagcatg tgccacaatg cctgcttata ccccccctct gtcacgccgg gcgcgctccg
       61 aacaaggtgt gaacaaccct caactctatc ttaatttctc ctgggtggtc attactttct
      121 cggacctgcc gcgttaacaa cgtcgaggcc gagatgcggg gacaagcgcg cccgtgtaac
      181 tgttgggcgt atgcctatta acattcgtta aataagtcct cccgatgtcc tccctacaca
      241 acaaccctcg acgtgggagg accgggttgt cccggcccct acatggagcg cagcaatgca
      301 gccgaacaac ccgagcatac ccggcccgga tacgcgcccg gcacgggtac tcatgtactg
      361 ccacgatggt tttggcctgg gccacttgcg ccgcaccacc accctggcac agaacctccg
      421 agacgcttac cccaatagcg gcatcctgct cgcggtgggc agcgcctcgc tccccttctg
      481 gaaagacctt cccggcctcg actatatcaa gctgccttcg attcgcaagg tggcgaccga
      541 ttgttggagc gcccgcagcc tgcccatcag cgccgacatg gcccacggca tacgcgcctc
      601 cgtgttgcgc gaggtgatgt tgcgctacca gcccgacctg ttcgtagcag attacctgcc
      661 cctcggcccc gccggagagc tggtgcccgc gctcaaggcc gcgaaggcca tcggcacccg
      721 ctgcgtgctt gggctgcgcg acatcctcga cgcccccgac acggtagcga ggctctgggc
      781 gcagcacggc atcgcggagg ccattgaaga gtggtacgac cgggtgctgg tctacggcga
      841 cccaggcgtg ttcgacagcg tgagcgccta cggcttctcg gcgaagatag caagcatggc
      901 gcggtactgc ggctacgtgg tcaacgactc gcctcacgaa gcaccggaaa aggtgcgggc
      961 gcggctcaac ctggcaacgt cccggctggt gctcgtgacg ggcgggggag ggcaggacgc
     1021 ggccccgctg gtgcgctctt accttcgcgg gctgcgggag cacgtgcaga gtcacgacgc
     1081 accctccttc gacagcctcg tcgtgcttgg gccgctgatg gaccgcaaca cccgcaacgc
     1141 gatacgcgag gaggcccagg ggctacccgg cagcatcagg accgaggtgg gcgacatccg
     1201 gcggtacgtg gcggcctccg acctggtggt cacgatgggc ggctacaaca gcctgatgga
     1261 ggtggcgctg gcccgcaagc ccgccgtggt ggtgcccagg cacgggccga gccaggagca
     1321 gaagatgcgg gccggcctgt tcgcctctcg cgggtggctg gtggcggcac ccgacagcga
     1381 gaccataacg gggcgcgagc tactgggcat aatcgagcgc accctggacg acccgccgcg
     1441 gggcgcgtgg gaaggcttcc gcatggaggg cgctcataag tcggtggagg cgcttgccga
     1501 ttgcctggcg gacaggcacg ccggccgcct ggtgaccttg ccggagatcg gctacagcct
     1561 gccgcatggg aggctccgca agagctacgg cccaggacgg acgccctcca tacgcccgcc
     1621 atacgcagac ggctcgccgc ccccgcaatt gcagtagccg ccgcgtgcga agcggcaccc
     1681 tgctcgctgc acgcctaacg atttgccacc tcccaaacaa cagatacagt tttgaaaggg
     1741 gacatgccgg ccatgtcgaa tacatacgag aaccgacctt tacgagtggg gtacgtactg
     1801 aagaggtatc cgcgctactc cgagacgttc gtggtgaatg agatactggc gcacgaagcg
     1861 gccggggtgc agatagacat cttctccctc cgcccgccta acgacgacca cttccaggac
     1921 gttatttcgc aggtgcgtgc gcccgtcacg tacctgccct cctacggctt gaaggcggcg
     1981 gacttctggc aggccatcca ggatgccaac ggcctgctgc cgggggtgtg caccgggctg
     2041 gaggcggcgc ggggcgagga cgtgcgcgac gtgcaccagg cgctgatgct cgcccgcgag
     2101 gtgaaactgc gtggcattac ccacctgcac gctcatttcg cgagcgtggc tacgagcgtg
     2161 gcccggctgg ccgcccactt cgcgggggtg ccttacacgt tcacggccca cgccaaggac
     2221 atctttcatg agagcgtgcg caaggacgac ctccggcgca agctcgagga cgcggcgggc
     2281 gtggtcacgg tcagcggcta caacgtggac tacctcagtg agatatccgg tgcggcgcgc
     2341 aacaagatca accgcatcta caacggcctg cacctcggcc agttccccta cacctcgccg
     2401 caggaccgcg cgccgctgat tgtgggcgtg gggcggctgg tcgagaagaa gggcttttgc
     2461 gacctggtct acgcgtgcgc cttgctggcc cgcaagggcg tggacttccg ctgcttgatt
     2521 atcggcactg gcgacgagga agccaggctg cgcgagcaga tagagaggct gggactcaca
     2581 gatagggtcg agcttatggg gccgaagccg cagtgcgagg tgaagcagta cgtgggtagc
     2641 gcggcggtgt tcgcggcccc ctgcgtggtc ggctcagacg gcaaccgcga cggcctgccc
     2701 accgtgctgc tggaagcgat ggccctgggc acgccctgca tctccaccga cgtgacgggc
     2761 atcccggagg tgctgcgcga cggcaagacg gggctgatgg taccccagca caaccccgcc
     2821 aaactggccg aggctatcga gcgcctgctg aacgaccctc agttgcgcgt gaatatcgcc
     2881 cgcagcgcgc gcaggctcat cgaggacgag ttcggcatcg accgcaattc agtgcgtata
     2941 cgcgccctgt tcgagcaagc cacagcggca aacgtgcttg agccggtgga agcggtagga
     3001 gcccgggcgt gagcatgcgg gtggcttacg tgtgcgccga cgccggtata cccgtgtttg
     3061 gcagcaaggg cgcttcggtg cacgtgcagg aggtaaccgg ggcgctgcta ggcagagggg
     3121 cgcgggtgac cctcttcgcc gcacgcaccg ggggcgagcc accggaggga cttgagggcg
     3181 tgacggtgta cgacctgccc acccccgcac agggcgagcg agcggagcgg gagcgggccg
     3241 cgttcgaggc caatgggatg atacgcgagg ccatttccca ggccgggcct ttcgacatgg
     3301 tatacgagcg gtattcgctg tggagctacg ccggtatgga gtacgcccgc gagcatggga
     3361 tacccggtct gctggaggta aacgcgccct tgatagagga gcaggccgag caccggggcc
     3421 tggtgcaccg cgagctggcc gtggaagtgg ctgacagggc cttcggggcg gctaccgccc
     3481 tgcttgccgt ctcgcgtgag gtggccgcgt acctggacgc gttgcccagc gccaggggtc
     3541 gggtccacgt tgtgcccaac ggtgtcaatc ctgggcggtt tgagggcgta gaaggcgctc
     3601 gcgacaggaa ggaagatggc accttcacgg tggggttcgt gggcacgctc aagccgtggc
     3661 acgggcttcc ggtgctggtc gaagctttcg atttgctctg ccgccgcaga cacgatgccc
     3721 ggctgctggt cgtgggcgag gggccggagc gcgagatgat gcaggcggaa ctggagaggc
     3781 gtggcctgtt gcgtcacaca cggttcaccg gcgcggtggc cccccacgag gtgccgcaac
     3841 tgcttgccga catggacgtg gccgtagcgc cctaccccgc cagggccgat ttctacttct
     3901 cgccgctgaa ggtgtacgag tacatggctg ccgggctgcc ggtagtcgcc agcagaatcg
     3961 ggcagttgga ggacgtaatc gagcacgaag ccaacggcct gctctgcgcc cccggcgacc
     4021 ccatagccct ggcgggtgcc ctggacaggt tgcggcagga cgcggctttg cgggcgcggt
     4081 tgggacagga ggcccggcgt agcgtgctca ggagccatac ctgggactcg gtggccgcgc
     4141 gcatacttga aatagccgcc acgaggcaag cgctgagtgg cgtcatggaa agggtgggtc
     4201 cgtagcgatg tcgcgtgcga tgacgctgaa ggagacaata ccgggcttct ggcgcatcat
     4261 ccggcggttc tggccctacc tgcgcaagca gcggaagctg atggcggggt cgctcgtggc
     4321 cctgtttgcc gaggtgggat tgcggctgtt ggagccgtgg cccctcaagt tcgtattcga
     4381 ccgcgtgatt ccccagcagc ccggcgtcac ctcttcgggc atccccctgc tcgactcgct
     4441 cgacgtgcgc atgctgctgc tggtgtcggc ggtgggcatc gtgctcatca cggcgatgag
     4501 ggcgctggcg gcctacttca acaccgtggg ttttgccatg ctcggcacga gggtgctgtc
     4561 gcagacgcgc agcgacgttt acaaccacat ccagcggctg tcgctctctt accacacgaa
     4621 agcccggagc ggcgacctgg tagtgcgcat catcagcgac gtgggccagc taaaggacgt
     4681 ggccgtgacc gcgctgctgc cgatgctggc gaacgtgctg atactggtgg ggatgctcgc
     4741 cctgatgctg ttgctccaat ggcagctcac actcctcgcc ctggccgtcg cacccctgtt
     4801 cgcgctcgct accgtgagga taagccgccg catacgcgag gccgcccgca cccagcgcaa
     4861 gcgcgagggc gcgatggccg ccaccgcctc ggagtctatc agcgcgataa aggtggtgca
     4921 ggcgctatcg ctggaagaca cgctcgctga gagcttcttc agccagaaca acaagagcct
     4981 caagcaggac gtgaagacca gcaagctggc ggccagcctg gagcgcacgg tagacgtgtt
     5041 gatagcgctg gcaaccggcc tggtgctctg gtatggggcc aacctcgtgc tggatggggc
     5101 gctgagcgcg ggcgaactgc tggtgttcct cacctacctc aagaacgggt tcaagccggt
     5161 gcgcgacttc gccaagtaca ccgggcggct ggctaaggcc accgcggcgg gcgagcgggt
     5221 gatggacgtg ctcgaacagg agccggacgt gcgcaacatg cccggcgcgg tacccgcgcc
     5281 cgcgctacgg ggtgccgtac gcttcaacaa cgtcgccttc gagtacgagc ctgggcaccc
     5341 ggtgctccgc gatatcaacc tcgacgtgca gccggggcag catatcgccc tggtgggggc
     5401 gtcgggcaac ggcaagtcca cgctggtgag cctcatcatg cgcctgtacg accccaccga
     5461 gggcagggtg ctgctggacg agcgggacgt gcgcgagtac accatcgact cggtgaggtc
     5521 gcagatcagc gtggtgatgc aggacaccat cctgttcgcg gcgagcgtgc gcgacaacat
     5581 agcctacggc tcgcccgagg ccaccgagga ggaaatagag gcagcggccc gcctcgccaa
     5641 cgcccacgac ttcatcgagg cgatgcccga aggctacgac acgatgctgg gcgagcgcgg
     5701 ggccaccctc tccaacggcc agcgccagcg catctcgatt gccagggccg ccatacgccg
     5761 cgcgcccatc gtcatactgg atgagccgac cacggggctg gacgaggaga acgagcgtac
     5821 cgtcatggag gccctggacc gcctggcgcg tggccgcacc acgtttctga ttacccacaa
     5881 attgagccag gcccgcaaag ctgaaaccat cgtcgtattg caagatgggt cgattgtcga
     5941 gcagggcacc cacgccgagt tgatgagggc aggtggtgcg taccggaagc tctacacggg
     6001 cgagtcgctt gccggaccgg gtgtagagat agccgttcct gccgcaagtc cggcccccgc
     6061 gcccgccgcc gtgcacatgg tggtgggagg cgaagatacc ggcgatgtca gcgatacggg
     6121 cagtttgcag accagtggcg gacccgtacc tgaaacccag ttggagggtg caaacaaggt
     6181 gaaggctcaa gcttataact caaaagaaac ggacagggcg cgcaagcctc gtgggtggag
     6241 gcgttctttc gccctcatgc tgggcgcgct cgcggtcacg gtggccctgg tagcggcggc
     6301 cataaatgcc aacgccaccg gcagcgcgac gaacggcgcg agcacggtag cgcaggcgtc
     6361 caccggggcc ccggctacga gcacggtgcc ttcgcgccgg gccgtgccta ccgccctcgc
     6421 ggatatgcgc tcgcacgtgg aagccacggt ggcggggggc gcggtacagg caacgagcgt
     6481 ggtcacctac tcgggctacc tgacgggcgt ggcttcgctg gcctggtcgc ccgacaacag
     6541 gacgctggcg ctcggctcca cggacgacgt tgccaggctg ctcgacacgc agaccggagt
     6601 ggtatcgatg ctgaaggggc acacgaagga tgtgtacctc gtggcctggt cgcccgacgg
     6661 cacgcgcctg gccacgggcg ggttcgacaa ccgggtgcgg gtgtggaccg ccggcgggca
     6721 gctagtctcc acccacggcg accattcggg catcatcgcg ggcctcacct ggctgcaaga
     6781 cggccgcagc ctcgcctccg tctccaccaa catgcagatc atccagtggg acgccgagtc
     6841 ggcctcgcag gtgttcgcgg tcaagggaga aggcgatttc ctctacaacg tgacctggac
     6901 cccggacggc agcatcatgg cggcaggggc gagcgatacg gtgcgcctgt ggagccgtga
     6961 gggcaaattg ctcaccgtca tgtccgggca cacggacgag atatactgcg ccgcctggtc
     7021 gccggacggc aaggtactcg ccacgggcgc taacgacgac acggtgcgcc tgtggagccg
     7081 cgagggcaag tctctggcga cccttaccgg gcatacggag ttcatcaacg gggtagcgtg
     7141 gtcgcccgac ggcaagcggc tggcctccgg ggcgttcgat aagacggtgc gcctgtggga
     7201 tgtggccgga aagcagctag gcaccctggt ggcgggcgcg gaggtcttgg cggtggcgtg
     7261 gtcgcccgat ggcaagagcc tggccgccgt gctgctggac ggcacggtgt gggtctggaa
     7321 gctggcgtaa gaggagggtg ggcgtgcttt cacgttacga cgcggaactg gtgcggcggg
     7381 acacggcgat ccccggtctg gctaccctgc tggatgccga ggcgttcgcc gcgtgcctcc
     7441 gagagcacgc cccaggagct gatatacgcc gcgtgcgccc cttctacgtg cggtacaagc
     7501 cggggatgaa ctgcctggtg gcgtacaagg tcgagctacc cggcgggcag gtagacatct
     7561 acgctaaggg gcacggggcc ggggccgccg acccgctacg cgaggcggtt gagcagccgg
     7621 gcataccggg ggcgctgggg cgcgggcgca tctccatgcg ccacctgggc atagtggtct
     7681 ccgtcttccc caacgacagc aagctagagc agctagaccg gctcggacgg ccggatgaaa
     7741 agacgcggct gctgcggagg gtcttttcgg accggcccga gctgtgggag gccgaagtgc
     7801 ggggcatcag gtacaagccg gagcgccgcc tggtggccca actaatgtcg ggagacaagc
     7861 cccaggccgt gctcaagttc tacagcgaca ccggctacgc ggacgctaag cagagcgcca
     7921 gggtgttcaa cacgcgcaag gccgactgcc gggtgttgca ggtgccccgc aggctgggcc
     7981 actccgaccg gcaccacgtg ctcgccttcg agtggatgga agggcaactg ctgagcgagg
     8041 cgcttgcggg ggcggacctg gaccttgcgg cgatagagcg tgtgggggtg gccctggccg
     8101 agggacacag gcggcccgtg ggcaagctgg cgccgctcaa ccgggagtcg gaagcctcta
     8161 cgctgctgtc cgtggcttcg gggctggcct tcctctgccc agacctgtcc gagaaggtgc
     8221 acggtttggc gcggcggctc gctacctacc tcatgcaaca gcctcctatg cacacgcccg
     8281 tccacggcga cttttacgcc aagcaggtgc tcctcgccgg ggagcgcgtg gtgatattgg
     8341 acttcgacag cgcggcggcg ggcgacccgg cctacgacct gggcctgttc gtggcccacc
     8401 tcgaacgcgg cgctatccgg cacacggtcc cgcaggacaa aatccactcg gtagccaatg
     8461 ccctcctcag tgggtacgag cgggagagcg ggcagacctt tcgagaacgg gttatggtgt
     8521 acgcggccgc cgggctgttg aagcttgctc cccaccagtt ccgcaaccgc gagcggaact
     8581 ggcccgaagg gatggaggcc atcatcgacc gggcagaggc cgtcctgagc agcgtggtag
     8641 ctcccgccgt tatcgccctt gcccctggag cgcaagggga ggagccggag cgtgttggcg
     8701 tgcaagcgaa cggggccgtg gaggcggcga ttgtagccgt cagcgacccg tttggtgcgg
     8761 tcaacgaccc cgcaatgcct ttcctggcgc aggcgctcaa cccggcggac gtgcaccagc
     8821 agttccggcg gcagttgtcc ggcttcatag gcgaaggggg ccggttggac ctcctgggca
     8881 tacgcgtcgt gaggcacaag atggggcggc gctgcctggt cgaatacaac gtggcggtgg
     8941 agaggccggg agagcagccg caggtggtcg cgctgattgg caaggcccgc gcgcgtggcc
     9001 tggacaaagc cacttttgag acggtatccg cgctgtggcg gtcggggttc gggggcgacg
     9061 cggaggacgg cattggcgtg cctgagcctg taggcacggt gcccaagttc cagatgtggc
     9121 tgcaccgcaa agtgcccggc gtaccggcta cgcaattgct ggccgcaaag gatggggcgg
     9181 acctcgccgg gcgcatagcg gaggcggcac acaagctgca ctcggcccgt atccgcccgc
     9241 aaaagcgaca tggcatggca gacgagctac gcatcctgca cgagcggttg cccctgctgg
     9301 gccagatgag gccggagtgg gcggggcgca tcgagcggct gctggctgcc tgcgaccgcc
     9361 tgggtgcggc attgcccagg ccgcgcaact ccggcatcca ccgcgatttc tacgccgacc
     9421 aggtgatggc gcatgcggac cgcatctacc tgctcgattt cgacctctac tgcgagggcg
     9481 atcccgccct ggacataggc aacttcctgg ggcacatcca ggagcaggcg ttgcgcacgt
     9541 tgggcgaccc gcgcgtcctg gcgcatgtcg aagaggcgtt ggaggagcgc ttcgtggagc
     9601 tttcgggcga gcagacacgc cgggcgatac aggtgtacac caccctcacc ctggtgcggc
     9661 acatacacat aagcacccgc ttcccggacc ggcagcagat caccgaggcg ctgtttgagc
     9721 tatgcgaaca gcgcctggcc cgacccgcag ctctgcccaa caggaggttg ggtgcgcggc
     9781 ccgcacaact ggtactgcag gaagggagcg tatagaggct gtatgaaggc aaggaagtca
     9841 ccgcttgcac gtgggctgct agtggccctg gtagccgtca tggcggcagg gtgctcccag
     9901 gagagcctca ccgggctgag gcagggtgtg gcaacgccaa tcggcggccc agcagagtcg
     9961 ccttcgcagg taccggaatc gtccaacacg cccggtgcag tgggggaagc cccaactcga
    10021 acgccacagc ctcgcgccag ccgcactgtc cccacaggta ccccgcagtt accacccgac
    10081 aacgacgacg gtgaggaagc agtgctggta ggggccgggg acataggcga ctgcgacagc
    10141 ggcgatgacg aacgcaccgc cgcgctgctg gatgagatcg aaggcaccgt ctttacgctg
    10201 ggcgacaacg tatacgaccg ggggaccgcc agggagtttc gtgagtgcta cgagccgagt
    10261 tggggacggc acaaggagcg cacgcggccc tcacccggca atcacgacta ccgcacggat
    10321 gacgcaggcc cctacttcga ctacttcggg gactcggcag gcgaaaaggg caagggctac
    10381 tacacctacg acctgggtag ctggcacatc gtggtgctga acagcaactg caagtacgtc
    10441 ggcgggtgcg aagaggactc ggagcagctg cgctggctgg aggacgacct cgcgaacaac
    10501 cccgcgcgtt gcaccctggc gtactggcac caccctctat tcaacgccgg ggagcacggt
    10561 aacgaaaagg ccgtgcgtcc gctgtgggag gccctgtacg atgcgggcgc ggaagtggtg
    10621 ctggctggcc acgaccacaa ctaccagcgt ttcgcgcccc aggaccccgg tggcaagagc
    10681 gagcctgagc gcggcatccg ccagttcatc gtgggcaccg ggggcaagga ccactacgac
    10741 atagaggact ctatgcccaa ccttgaggcc agcaacgacg acgcgaacgg cgcgctaaag
    10801 ctcactctgc gcgcagaggg ctatagctgg gagtttattc cggccgagga agatgacttc
    10861 caggatagcg gcacaggaag ttgccactag gcagagagtg cttcaccatg gtcccctgaa
    10921 gcaacataga cgccagccga cgcccatcga ttgaaatcga gggctacaca ttgcaaagtc
    10981 ccacaagggg actccttcat agcaacattt acgtgcagga gagacaccaa
//
LOCUS       DASRWZ010000113         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1243094, whole genome shotgun sequence.
ACCESSION   DASRWZ010000113 DASRWZ010000000
VERSION     DASRWZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243094"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFH60_01660"
     CDS             complement(<1..736)
                     /locus_tag="VFH60_01660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6312515.1"
                     /translation="MRNISRRELLGLAGLGALGLGAAALGIDRLAPRSGGEKEGEGDM
                     LTVFTFGDSILDCGHYNQYGVHPGQLVVRNDDRLFPEFKGNDLTSRGEARLQHRAVDG
                     ATVNGLPSQARGLKVEGPAVAMLTVGGNDLLQGLVIDDGSGIKEFAQKLEGFLKQLPI
                     RPVLVGNVYDPSFGDDNQNVLGVDPRLARENHRRMNAVLAEMGDKYGTLVDLHAHFLQ
                     GDPSWYTHTIEPSLTGASEVRRAFLPH"
     gene            complement(743..2611)
                     /locus_tag="VFH60_01665"
     CDS             complement(743..2611)
                     /locus_tag="VFH60_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312516.1"
                     /translation="MFGRRLSLAIVALLLALLPVLSAGAANRYAPLAASMEGWSAPVN
                     ISNSKQYDNTPSIAASPNGAVSIIWLRSQGANPNANLLLQARNSSLGGGFQQEQLAES
                     VPQKSSGGAIVRRDALGRKHIIWWQYEGRAVCDYYLRIEADGRTSIKEQVPGTCGHSY
                     KNTALAIGPDNNAHMLFGQNLQSLFYWVRADAGWPVQAERIGGTTTPEAPTLGVTTQG
                     TAIAAWKDKAPGGFGDVYTGVRQAPGKWQVENVSHTLTPGCEGNSITDEPALAADPTG
                     GMRLAWEDERCDPRNPEPTLREPYYREWVPATGWNALPPVRVHRTPGNAFGIAITVDA
                     GGTAHIVWATVLRERCTLYYAGGRGNRFSDPATPFDGWARGEAIKDMSLAYSPGYLHL
                     AFGSPRDDPDKENYYTYLQIGDPPPPPTPTPPPLPKLPPSNEKVPGNGSRAFPETGYT
                     VTGVFLDYWNAHGGLRQQGFPISNVVRETSPLDGKPYLMQYFERAVFEYHPENPAGSN
                     VLLSQLGTFQYRRKYPNGAPNQRPNQDAVAPFPETGHRVGGSFLQYWQQNGGLAQQGF
                     PISDEFTEVSETDGKTYTVQYFERAVFEWHPANPPPNNVLLSLLGVFRLKQQYGGG"
BASE COUNT          456 a          867 c          997 g          570 t
ORIGIN      
        1 tgtgcggaag gaaggcgcgg cgcacctcgg aggccccggt gaggcttggc tctatagtgt
       61 gggtgtacca ggaggggtcg ccctggagga agtgggcgtg caggtccacc agcgtgccgt
      121 atttgtcgcc catctcggcc agcacggcgt tcatgcggcg gtggttctcg cgggcgagcc
      181 tggggtccac gccgaggacg ttctggttgt cgtcgccaaa gctggggtcg tagacgttgc
      241 cgaccagcac cgggcgaatt ggcaattgct tcaggaaccc ttccaacttc tgcgcgaact
      301 ctttgatgcc cgacccatca tcaatgacca gcccctggag caggtcgttg ccccccaccg
      361 tgagcatcgc cacggcaggg ccttctacct tgaggccgcg cgcctgcgat ggcaacccgt
      421 ttaccgtggc cccatccacc gccctgtgtt gcaggcgggc ctccccccgc gatgtcagat
      481 cgttgccttt gaattcgggg aagagcctgt cgtcgttgcg cacgaccaac tggccagggt
      541 gcacaccgta ctggttgtag tgcccgcaat cgagaataga gtcgccaaat gtgaagaccg
      601 ttagcatgtc gccttctccc tctttctccc caccgctgcg tggggctaac ctgtctattc
      661 cgagtgccgc ggctcccagg ccgagcgcac ccaggccagc aagtccgagc aactcgcgcc
      721 tcgaaatatt cctcatgcgc gactatcctc caccatattg ctgcttgaga cggaacacgc
      781 ccagcaatga cagcagcacg ttattgggcg gcgggttcgc cgggtgccac tcgaacacgg
      841 cgcgctcgaa gtactgcacc gtgtaggtct tgccgtccgt ctcgctcact tcggtgaact
      901 cgtcggaaat ggggaagccc tgctgggcca ggccgccgtt ctgctgccag tactgcaaga
      961 aggagcctcc gaccctatgc cccgtctcag gaaacggtgc cacggcatcc tggttgggcc
     1021 gctggttggg tgcaccgttg gggtacttgc ggcggtactg aaacgtgcca agctgcgata
     1081 ggagcacgtt gcttcccgcc gggttctccg ggtggtactc gaacaccgcg cgctcgaagt
     1141 actgcatcag gtagggcttg ccatcgaggg gcgaggtctc ccgcaccacg ttggagatag
     1201 ggaagccctg ttgccgcagg ccgccatgcg cgttccagta gtcgaggaat acgcccgtga
     1261 ccgtgtagcc ggtctccggg aaggcgcggc tgccgttgcc gggcaccttc tcgttggagg
     1321 gtggtagttt ggggaggggc ggaggcgtgg gcgtgggcgg cggtggggga tcgcctattt
     1381 gcaagtaggt gtaatagttc tccttgtcgg ggtcgtcgcg agggctgccg aaggccaggt
     1441 gcaggtagcc ggggctgtag gcaagggaca tgtccttgat ggcctcgccc cgcgcccagc
     1501 cgtcgaaggg cgtggccggg tcgctgaagc ggttgccgcg cccgccggcg tagtagaggg
     1561 tgcagcgctc gcgcaacacg gtggcccaca ctatatgcgc tgtgcccccc gcgtctaccg
     1621 ttatggcgat gccaaaggcg ttaccggggg tgcgatgcac gcgcacgggg ggcagggcgt
     1681 tccagccggt ggcgggcacc cactcgcggt agtacggctc gcgcagggta ggttcggggt
     1741 tgcgggggtc gcagcgttcg tcctcccacg cgaggcgcat gccgccggtg gggtccgcag
     1801 ccagggcagg ctcatctgtg atggagttgc cttcacagcc gggcgtgagg gtgtgcgaca
     1861 cgttctcgac ctgccacttg ccgggggcct gccgcacgcc cgtgtagacg tcaccgaagc
     1921 cgccgggggc cttgtccttc caggcggcga ttgcggtgcc ctgggtggtc acgccgagcg
     1981 tgggggcttc gggcgtggtg gtgccgccta tcctctcggc ttgcacgggc cacccggcgt
     2041 cggcgcgcac ccagtagaag aggctttgca ggttctggcc gaagagcatg tgggcgttgt
     2101 tgtcggggcc gatagcgagc gcggtgttct tgtagctatg cccgcacgtg ccgggcacct
     2161 gctccttgat ggaggtgcgg ccatcggcct cgatgcgcag gtagtagtcg cacaccgcgc
     2221 ggccctcgta ctgccaccag atgatgtgct tgcggcccag cgcgtcccgc cgcacgattg
     2281 ccccgccgct cgacttttgc ggcacgctct cggctagctg ctcctgctgg aatcctccgc
     2341 cgagggagga gttacgggct tgtagcagca ggttggcgtt ggggtttgcg ccctggctgc
     2401 gcagccagat gatgctcacc gcgccgttgg gggaggccgc gatggagggc gtgttgtcgt
     2461 attgcttcga attggagatg tttacgggcg cgctccatcc ctccatgctc gcggcaaggg
     2521 gcgcgtacct gttggctgcc ccagccgaga ggacgggcag gagggccagc aacagcgcga
     2581 ctatcgcgag agataagcgt ctaccaaaca acgagtgcgg cgtcccttct agaaggctcc
     2641 ctttagcgtg gagtgggtca tgaacagata cgtaagtgta gtgttgtaat tgtaccaaga
     2701 agggacgtgc attcgctgtg ccaagtttgc ccaggtcggt gatttcttca acctgtactc
     2761 ccctttgtca tcctgagcgt agcgaaggat caggtgggta ggccagagtc ttcaacgttg
     2821 ggaatgatgg ccgcattagg aacattgggg ggcttcaata ggagcagtca agaacgggcg
     2881 gctgatcctt
//
LOCUS       DASRWZ010000114         5319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_694978, whole genome shotgun sequence.
ACCESSION   DASRWZ010000114 DASRWZ010000000
VERSION     DASRWZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694978"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFH60_01670"
     CDS             <1..486
                     /locus_tag="VFH60_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6312517.1"
                     /translation="FSEPVDAVSFTDVPPGSAFYLYVERLRHRGVVNGYACGGKNPQT
                     REDEPCDDLGRPYFRPGNTATRGHTARIVSQAGQVPDPIDRRPAHFADVEPGDPYYTE
                     VERLYGIGAIGGFPCGGPGEPCNDANQPYFRPGASVTRGQATKIVSNTFYPNCQAPAR
                     R"
     gene            complement(632..1354)
                     /gene="radC"
                     /locus_tag="VFH60_01675"
     CDS             complement(632..1354)
                     /gene="radC"
                     /locus_tag="VFH60_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET6312518.1"
                     /translation="MPHDSTVPNGRANFTIKELPLDARPREKLKALGAGSLSESELLA
                     IILRVGTQGESVVDLANRLLVTYGGLVGLSRVSFPELCGVHGLGEAKACQLKAALELG
                     RRLLLAQPEERLQVRSPADLAPLLMLEMAPLDQETLRVVLLNTRNQVIKFADVYRGSL
                     NSSLVRVAEVFKEAVRVNAAAIIVAHNHPSGDPTPSPEDRLVTEQIVAAGKLLEIVVL
                     DHLVIGERRYISMREKKLGFRG"
     gene            complement(1542..1832)
                     /locus_tag="VFH60_01680"
     CDS             complement(1542..1832)
                     /locus_tag="VFH60_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET6312519.1"
                     /translation="MKQPKEYYVYIMTNSTRVLYTGVTNNLMRRVYEHKEGAKPGFTS
                     RYKVEWLVYVEVTDDVRDAIAREKQVKGWVRAKKIALIESLNPGWRDLSLDW"
     gene            2118..2990
                     /locus_tag="VFH60_01685"
     CDS             2118..2990
                     /locus_tag="VFH60_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312520.1"
                     /translation="MRHDLASKNGAPPTLGPLTTGLSLAEVVARLSKKPAVDGVLVMG
                     SGGTPDMHPASDYDLFVVLNAMPAPLYLVATEIDHRLAEIYFGSTEKLQEYIAAEPPL
                     QPVDIDAAVTRWLQTGRVAFDRSGLLSRAQARFRQREWQDWFKPQTGLQLHQTWFSVN
                     YNLAQTRRMLASPDPVYRTAVDLRLLFTVMEVWQAYFKLRRLPSLGEKHDVRYLAAND
                     PEYLDLFRCCLAEPDRSRKFELYGRLADIALAPMGGLWQEGTTAMQLRGDTQWQPDTV
                     DNALAFWRELVTEG"
     gene            3001..4023
                     /locus_tag="VFH60_01690"
     CDS             3001..4023
                     /locus_tag="VFH60_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6312521.1"
                     /translation="MQATPGVQSLYSQLFDAEFMRKLERLSLISRKLKAGRMKGERRS
                     PRRGQSVEFADYRTYAPGDDLRRVDWNAYARMERLFLKLFQEEEDITVHILIDASKSM
                     DWGDPNAVTLTSAVATVDRGQGTDAHGAVVKGSEGLDTSGQNKLVYAKRVAAALGYIS
                     LSNLDRLAVAGISKAGVQRFTAVRGKGHAVSLLRFVAGVRAEGETDLDASLRQYASQA
                     RYPGLLYVLSDCLMDGAGLEGLKALQASGHEVHLVHILAPSEVNPELALMGDLRLRDV
                     ETGATQEVSIESGILDLYREKFEQWQDGIESFCRRRGINYLQVTTDLPFEDLVLQYMR
                     QRGVVG"
     gene            complement(4338..5255)
                     /gene="larC"
                     /locus_tag="VFH60_01695"
     CDS             complement(4338..5255)
                     /gene="larC"
                     /locus_tag="VFH60_01695"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HET6312522.1"
                     /translation="MRVAFFDPVSGLSAEMILGALIDVGVPLESLRAELYKLPLSGYS
                     FDLAKALRQGVTGTYLQVGTGPASHNGAGQLAGRLGRHTLAKPPDSSLDVAAMLRMIL
                     GSSLPAPVQQASVKVLRNLAETESELAALHRQNGHANAIWNVETLAQVVGTMSALNML
                     GVNRVDCAAFGVGCDYDSLPAHDRLGIALLRRTPRACDENKIDRGYVTLTGAAIMVSI
                     VTSFGRVPTMRIDGVGYGATTTAEPGRSGLLRVLIGDTTACPTDDSQTTIPLMLTPTN
                     GHHSTAPADTLIAGSHQQSGRHRLLKRET"
BASE COUNT          951 a         1784 c         1593 g          991 t
ORIGIN      
        1 ttcagcgagc ctgttgacgc cgtgtccttc accgacgttc cgcccggcag cgctttttac
       61 ctctacgtcg agcgactgcg ccaccgcgga gtggtcaacg gctacgcctg cggcggcaag
      121 aacccccaga cgcgggagga cgagccttgt gacgacctgg gacgccccta cttccggccc
      181 ggcaacacgg ccaccagggg ccacacagcc cgcatcgttt cccaggcggg gcaggtcccc
      241 gaccccatcg accgccgccc cgcgcacttc gccgatgttg agcctggcga cccctattac
      301 acggaggtgg aacgcctcta cggcatcggg gccatagggg ggttcccttg cggcggcccc
      361 ggcgagcctt gtaacgacgc caatcagcct tacttcaggc cgggagccag cgtcacgaga
      421 gggcaggcca ccaagatagt gtctaacact ttctatccca actgccaggc acccgctcga
      481 aggtagagag taatgcgtaa cacataacgc gtaacacgta acgcggctca agagactctg
      541 taccatatga gtcgctttgg tgctcgtggc actattacgt gttacgcttt acgccttacg
      601 tgttacgtgt tactccctac tcccacatcg tctagcccct gaaccccagc ttcttctcgc
      661 gcatgctgat gtacctgcgc tcgcctatca ccaggtggtc cagcaccact atctccagca
      721 gcttcccggc agccactatc tgctcggtga cgagcctgtc ctcgggcgag ggggtagggt
      781 cgccgctggg gtggttgtgg gccacgatga tggcggcggc gttcacccgc accgcctcct
      841 tgaacacctc agccacgcgc accagcgagg agttgagcga accacggtac acgtccgcga
      901 acttgatcac ctggttcctg gtgttgagca gcaccacccg tagcgtctcc tggtctaatg
      961 gggccatttc cagcatgagc agcggggcca ggtcggcggg cgagcgcacc tgcaaccgct
     1021 cctccggctg ggccagcagc agccgcctgc ctaactccag tgcggccttc aactggcacg
     1081 ctttcgcctc ccccagcccg tgcaccccgc acaactccgg gaaggaaacc cgcgacagcc
     1141 ccaccagccc gccgtaggtc accagcagcc ggttcgccag gtccaccacg ctctcgccct
     1201 gggtgcccac ccgcagtatg atcgccagca actccgactc cgatagcgaa cccgccccca
     1261 gcgccttcag cttctccctc ggcctggcat cgagtggcag ttccttgatg gtgaagttgg
     1321 cgcgcccgtt cgggacggtt gagtcatgcg gcatagtaac ccctccctac tgccatttag
     1381 ctccgtaagt tctcatttca tcggcggcta aacgtggtcc caaagccata atgcctcctg
     1441 gctccttccg cagcgcccaa gagtgcccct gtcatcctga gcgtagcgaa ggatctaaag
     1501 aagttggcct agtacaatgc tggacctggc tgaacccgcg ttcaccagtc gaggctcaaa
     1561 tccctccacc caggattaag cgactcaatc agcgcgatct tcttcgccct gacccaacct
     1621 tttacttgct tctctctagc tatggcatca cgcacgtcgt ccgtgacctc aacgtagaca
     1681 agccactcaa ctttgtacct gctggtaaaa cccggtttgg ctccttcttt gtgctcgtat
     1741 acgcggcgca tcaggttgtt cgtgacgcct gtgtagagga cacgcgtgct gttcgtcatg
     1801 atgtagacgt agtattcctt cggttgcttc atcggggcta cccaacttct ttagatcctt
     1861 cgctacgctc aggatgacag gggcactcgg gtccgtcaca tcgcggcgaa gcggcttcct
     1921 gcgctgtgtg ctgctattgc ccgaatccgc cattgtctgt agactctact tgtgcaggaa
     1981 gcctcctatc gggccggcat gatgaacctc ccgcccatcg tgcctgtata tcgagtgtcg
     2041 ggtctcatgg attcacccgc ttccaccgcc cggcgacgtt agtagcctct atatagcttt
     2101 cccgcgcaag gagcgaagtg cgacacgacc tggcgagcaa aaacggcgca cccccgaccc
     2161 tcggcccgct cacaaccggt ctgagcctgg ccgaagtggt cgctcgcctg tcgaagaagc
     2221 ccgcggtgga cggcgtactc gttatggggt cggggggcac acccgatatg caccccgcca
     2281 gcgactacga cctgttcgtc gtgctgaacg ccatgcccgc acccctctac ctggtggcga
     2341 ccgagatcga ccaccgcctg gcggagattt acttcggctc tacggagaag ttgcaagagt
     2401 atatcgctgc cgagccgcct ttgcagccgg tcgatattga cgctgcggtg acacgctggc
     2461 tgcaaacagg gcgtgtcgcc ttcgaccgct ccggcttgct gtcccgggcc caggccaggt
     2521 tccggcagcg cgagtggcaa gactggttca agccccagac cggcctgcaa ctgcaccaga
     2581 cgtggttcag cgtcaactac aacctcgccc agacgcgccg catgctcgcc tcccccgacc
     2641 cggtctaccg gaccgccgtg gacctgcgcc tgctgttcac ggtcatggag gtgtggcagg
     2701 cctacttcaa attgaggcgg ctcccctccc tcggcgagaa gcacgacgtc cgctacctcg
     2761 ccgcgaacga cccggagtac ctcgaccttt ttcgctgctg cctggccgag ccggaccgct
     2821 ctcgcaagtt cgagctttac gggcggctcg cggatatagc cctcgcgcct atggggggcc
     2881 tctggcagga gggcaccacc gccatgcagt tgcgcggcga tacacaatgg caacccgaca
     2941 cggtagacaa cgccctcgcc ttctggcggg aactagtaac agagggataa cggagtaatt
     3001 atgcaagcaa cccccggtgt gcagtcgctc tacagccagt tgttcgatgc cgagttcatg
     3061 cgcaagctgg agcgcctgtc cctcatcagc cgtaagctca aggcaggccg catgaaaggc
     3121 gagcggcgca gccccaggcg cgggcagtcg gtggagttcg cggactaccg cacttacgcc
     3181 cccggcgacg acctgcgccg cgtggattgg aacgcctacg cccgcatgga gcgcctcttc
     3241 ctcaagctct tccaggagga ggaggacatt accgtgcaca tcctcatcga cgcgagcaag
     3301 tcgatggact ggggcgaccc caacgccgtg accctcactt cggccgtggc taccgtagac
     3361 cgggggcagg gcaccgacgc gcacggggcg gtggtgaaag gcagcgaggg cctagatacg
     3421 agcgggcaga acaagctcgt ctacgccaag cgcgtggccg ccgccctcgg ctacatctcc
     3481 ctctccaacc tcgaccggct cgcggtggcc ggtatcagca aggcgggggt gcaacgcttc
     3541 acagccgtgc ggggcaaagg gcacgcggta tcgctgctgc ggttcgtggc gggggtgcgg
     3601 gccgagggcg agaccgacct ggacgcctcg ctgcggcagt acgcctccca ggcccgctac
     3661 cccggcctcc tctacgtgct cagcgattgc ctgatggacg gggccggcct ggaggggctg
     3721 aaggcattac aggcgtcggg gcatgaagtc cacctggtcc acatccttgc cccctccgag
     3781 gtcaaccccg aattggccct catgggcgac ctgcgcctgc gcgacgtgga gaccggggcc
     3841 acccaggaag tgagcatcga gagcggcatc ctggatctat acagggagaa gttcgagcag
     3901 tggcaggacg gcatagagag cttctgccgc cgccggggca tcaactacct ccaagtcacc
     3961 accgacctcc ccttcgagga cctggtgcta caatatatgc gtcagcgggg cgttgtgggg
     4021 tagtgacgag tgacgagtga ggcggcttgc taatgtgggt acgagaacac gatcatgctg
     4081 gttgcgcggg accaaagtgg ccttgtcatc ctgagcgcag cgaaggatca atcccccgtg
     4141 tttgactctc cctattgaag caccagtgtt cctcttacca ccctcatccc caacgttgaa
     4201 gactctggcc tacccacctg agccttcgct acgctcagga tgacaggggg aggcttggtg
     4261 ccgcatagcc ggtagcgtcg tatttacgaa ccaacgccag caatcctcat cactcgtcac
     4321 gcattacgcg ttacgtgtta cgtctcgcgc ttcaagagcc tgtgcctgcc gctctgctgg
     4381 tggctgcctg ctatgagcgt gtcggcgggc gcggtggagt ggtggccgtt ggtgggggtg
     4441 agcatgaggg ggatggtggt ctgcgagtcg tccgtggggc aggcggtggt gtcgccaatt
     4501 agcaccctta gcaggccgga gcggcccggt tcggcggtgg tcgtagcgcc gtagccgacg
     4561 ccgtcgatgc gcatggtggg tactctgccg aagctagtca cgatggatac cataatggcc
     4621 gcgccggtga gggtcacgta gccccggtcg atcttgttct cgtcgcaggc gcgtggggtg
     4681 cggcgcaaga gggctatccc caggcggtcg tgcgcgggca ggctgtcgta gtcgcagccc
     4741 acgccgaagg cggcgcagtc cacccggttc acccccagca tgttcaaggc cgacatggta
     4801 ccgaccacct gcgccagcgt ctcgacgttc catatggcgt tcgcgtgccc gttctggcgg
     4861 tgcaaggccg caagctcgct ctcggtctct gccaggttcc tcaacacctt gaccgaagcc
     4921 tgttggaccg gcgcgggcag gctgctcccc agtatcatgc gcagcatcgc ggccacatcc
     4981 aggctgctgt cgggcggctt ggcaagggtg tgcctgccga ggcgtcccgc cagttggccc
     5041 gccccattgt gggaagcagg cccggtgccc acttgcaggt aagtgcccgt gaccccctgc
     5101 cgcaaagctt tagccaggtc gaacgagtag cccgaaagag gcagcttgta tagttcggcc
     5161 cggagcgact ccaggggcac gcccacgtcg attagggcac ccaggatcat ctccgctgaa
     5221 agccccgaca ccggatcgaa gaacgccacc cgcatacctg aagttttcca ctcctcccgc
     5281 cacggcacag ccccggcacg cgcgtattat agcacagcc
//
LOCUS       DASRWZ010000115         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_78316,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000115 DASRWZ010000000
VERSION     DASRWZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78316"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFH60_01700"
     CDS             <1..746
                     /locus_tag="VFH60_01700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6312523.1"
                     /translation="LTFPYNFALLFGVATVCIGVGVWAWSSVHEPPALVTAPPMSVRD
                     VVRRGVSAFKEDGDYRAFMVARILISLATIADPFYVVFAKTHLGAPPATVGLYLGAVA
                     VSSLLSNFIWSPLSDRAGNRTLMVLTVCSVALVPATALVLSLFAGILPSEVLFTAFAL
                     VFIVSGLALGAARIINNNMLLTIAPKAQLGTYVGFLNTVLGIVIFVPVLGGLLADLVG
                     YEALFALSMLLALSAFFSVRNMSPSSARS"
     gene            836..1573
                     /gene="rph"
                     /locus_tag="VFH60_01705"
     CDS             836..1573
                     /gene="rph"
                     /locus_tag="VFH60_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET6312524.1"
                     /translation="MAPTKRLDGRAADQLRPVQITPNFQLFPAGSVLIEFGRTRVICA
                     ASVEERVPPFMAGSGKGWVTAEYSMLPSATPTRSSRETSGSKPQGRTMEIQRLIGRSL
                     RAVVNPHALGERTIAIDCDVIEADGGTRTASITGAVVALALAVRKLQAQGTISAGSAP
                     VLKQLLAAVSVGIVNGVPMLDLNYQEDSQADADCNVVMTDAGWLVEIQATAERKAFSR
                     DELDRLLALATSGCNQLIRLQREVLGL"
BASE COUNT          262 a          497 c          512 g          314 t
ORIGIN      
        1 ggctgacgtt cccttacaac ttcgctctgt tgttcggcgt cgcaaccgtt tgcataggag
       61 tgggtgtttg ggcctggtcc tccgtgcacg agcctcccgc cctggtgacg gcgccgccca
      121 tgagcgtgag agacgtcgtg cggcggggcg tgagcgcctt caaggaggac ggcgactacc
      181 gcgctttcat ggtggcccgc attctcatct cgctggctac gattgccgac cccttctatg
      241 tggtgtttgc caagacgcat ctcggcgcgc cccccgcgac agtgggactc tacctgggtg
      301 ccgtggccgt atcgtccttg ctttccaact tcatctggag tccgctgtcc gaccgagcgg
      361 gcaaccggac gctgatggtg cttacggtgt gctccgtggc gctggtgccc gcgaccgcgc
      421 tggtcctgtc gctgttcgcg ggcatcttgc cttccgaagt gctcttcacc gccttcgcac
      481 tggtgttcat cgtctctggc ctggcgctcg gggcggcgcg cataatcaac aacaacatgc
      541 tgctcacgat cgcgccaaag gctcagctcg gcacgtacgt gggtttcctc aacacggtcc
      601 tggggatcgt aattttcgtg ccggtgctcg gcgggttgct ggccgacctc gtgggctacg
      661 aggcgttatt cgcgctgtcg atgctgttgg ccttgagcgc gttcttctcg gttcgcaaca
      721 tgagtcccag ttcggcacgt tcttagaggt tgtaggacag cagcatgccc aggcttgtac
      781 cccgaccgcg gtaggtgcaa gcaggctgaa acactacaca acaggaggcg tttacatggc
      841 cccaacaaag cggctggacg ggcgagcggc ggaccagctt cgacccgtgc agattacccc
      901 caacttccaa ttattccccg caggctcggt gctcatcgaa ttcggacgca cgcgggtgat
      961 atgtgccgcc agcgtcgagg agcgggtgcc gcccttcatg gcgggcagcg gcaaaggctg
     1021 ggtaaccgcc gagtactcga tgctgccctc cgccacgccc acccggagca gtcgcgagac
     1081 atccgggagc aagccccagg gccgcactat ggagatacag cggctgattg gccggtccct
     1141 tcgagcggtt gtcaaccccc acgcgctggg cgagcgcacc atcgccatcg actgcgacgt
     1201 gatcgaggcg gatgggggca cgcgcacggc ttccattacg ggcgcggtag tggcgctggc
     1261 cctcgcggtg agaaagctac aggcccaggg tacgatctcg gccgggtcgg ccccggtgct
     1321 gaagcagttg ctggcggcgg taagcgtggg catcgtgaac ggcgtgccga tgcttgacct
     1381 gaactaccag gaggacagcc aggccgacgc cgactgcaac gtggtcatga cggacgctgg
     1441 gtggctggtc gaaatccagg cgaccgccga gcgcaaagcc ttcagccgcg atgagcttga
     1501 caggctgctc gctctcgcca cgagcggctg caaccagttg atacgcctgc aacgtgaagt
     1561 cttgggactc taattctgat aaaat
//
LOCUS       DASRWZ010000116         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_215228, whole genome shotgun sequence.
ACCESSION   DASRWZ010000116 DASRWZ010000000
VERSION     DASRWZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215228"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3601)
                     /locus_tag="VFH60_01710"
     CDS             complement(<1..3601)
                     /locus_tag="VFH60_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312525.1"
                     /translation="MRKSVRIIGILVTLSMLLSLAPAGQPLGQAGVVDAATPADGTLN
                     PPNSQVTWTGGPYTVVTPDPALCSDSSTNCDTFTLNVNLPDNYWETAEGKVTIGITWT
                     GSADFDMYVYNEEGDEVDSSAAGATTYEEVTLPELPGGTYEVRVVAYTAFGTTYNGYA
                     RLESTQLPPGMVYPESRDVIMEALTVDYPLNIIFVGYSPTAEEIAEMRQWIPDNYQPT
                     VSSKSPIPDEIQNEAAGLLNWNKNHYINGKPYFLGIRYNYKIRFLQATEEYARALFQQ
                     AENSTAPNQSFHTAQVGASLARYDQSYGNYRVVAKGGDPSYKVTDPTKTDLIDAYAME
                     DWIFNSRYDDLWNCAFRDLETGDLYSAAVIQPGEDAYYDPYYDKNGLNLDRMPQGPNK
                     GSSFLFFDTFMPEYASEYFRPNAYHTWGTDKVINNAIVSKKVEEGGSWRITNPDTGAW
                     DGVDFARTWGGRYRFHFIDLGAAPNDYESSSWAGAERTMSSDYPHGDPPIWQYKADPQ
                     WQQSGEACAADDLNPQYEFTGGTPCRMMPRLGRDVAYGLFFRSTSGYLYRPIPRGDVN
                     WLAVSNWTDFYSRPQYDPVTGQITSAPWYGNWWTNTDKLYKIANATDPAATDDVLRWL
                     SSALPYTRWVGRKGEVITVYDPTNNQPTARLLDTSPKYEDLPAPEYHVSSSATDFEMV
                     PEPLYAFHTSPRIVTYGSSQVNLTDLSNAFEKAKARGFVGLTYDFSVPHEYMRDYFDA
                     HRPGIADIVTGTNGISEFSLVNTIPGINMVFEKAYTWALPVIVGGIAVGTDDGEAWGT
                     MNNVNDRAKSPLSDYPANPAANCPAPTRTLEPWNCPKHSTVADPGARSGGGFSYTIEH
                     EAAHNLGLSHPHDGSFGIDRCPEDDPNEARRGKWECYWSGLGWMYDISAAPTTYAMSY
                     RPYEVEDQDNLQRGRIAEYLVLGQDALKQRLIDESNDGNTTPSAGWTSDFNRMKQWRA
                     QAAALFQVGDYLHSEYAARNASLAARGVPQLPGNTSNPKLVEAGQIFYFNVNPQTQPE
                     EIVPCGGGGPGPVLPPCEIQFQDVPPSTDVSSFYPFVRCLACQHILGGYPCGGPGEPC
                     GVTGDPYYRPNANITRGQISKIVSNSARFHEDPGAQLDADVKPGDPFYPWINRLSRRH
                     HMGGYPCGGPGEPCDGSNRPYFRPASNATRGQLSKIVSNAASFNDTPQGQTYTD"
BASE COUNT          712 a         1140 c         1405 g          827 t
ORIGIN      
        1 cgtccgtgta ggtctggcct tgcggcgtgt cgttgaagct cgcggcgttg gaaactatct
       61 tcgatagctg cccgcgagta gcgttggagg cgggccggaa gtacggcctg ttgctgccat
      121 cgcacggctc gccggggccg ccgcaggggt agccgcccat gtggtgcctg cgcgacaggc
      181 ggttgatcca cgggtagaac gggtcgcccg gcttcacgtc agcgtccagc tgcgcgccgg
      241 ggtcctcgtg gaagcgcgcc gagttggaca cgatcttgga gatctgcccg cgcgtgatgt
      301 tggcgttggg ccggtagtat gggtcgccgg tcactccgca cggctcgccg gggccgccgc
      361 aggggtagcc gccgaggatg tgctggcaag cgaggcagcg cacgaacggg tagaaggacg
      421 acacgtcggt gctcggcggc acgtcctgga actggatctc gcaaggcggg agcacggggc
      481 cggggcctcc gccgccgcaa ggcacgatct cctcaggctg cgtctgaggg ttcacgttga
      541 agtagaagat ctgcccggcc tccaccaact tggggttgga ggtgttaccc ggcaactgag
      601 gcacgccacg cgccgccagg gaggcgttgc gggctgcgta ctcagagtgc aggtagtcgc
      661 ctacctggaa cagggccgcg gcctgcgcgc gccactgctt catccggttg aagtcggagg
      721 tccacccagc cgagggggtg gtgttgccgt cgttcgactc gtcgatcagc ctctgcttca
      781 gggcgtcctg gccaagcacc aggtactcgg cgatgcgccc gcgctgcagg ttgtcctggt
      841 cctccacctc gtaggggcga tagctcatag cgtaggtggt gggagcggcc gagatgtcgt
      901 acatccagcc gagacccgac cagtagcact cccacttgcc gcggcgggct tcgttcgggt
      961 cgtcctcagg gcagcggtcg atgccgaagg agccgtcgtg cgggtgagac aggcccaggt
     1021 tgtgggcagc ctcgtgctcg atggtgtagg agaaaccgcc gccgctacgg gcaccggggt
     1081 cggccactgt ggaatgcttg gggcagttcc acggctcgag cgtgcgggta ggggcggggc
     1141 agttcgcggc cgggttggcc gggtagtcgg acaggggcga cttggcgcgg tcgttgacgt
     1201 tgttcatcgt gccccacgcc tcgccatcgt ccgtgcccac ggcgatacca cccacgataa
     1261 cgggcagcgc ccaggtgtag gccttctcga acaccatatt gataccgggg atggtgttca
     1321 ccagggagaa ctcagagatg ccgttcgtgc cggtcacgat gtcggcgatg ccgggccggt
     1381 gagcgtcgaa gtagtcgcgc atgtactcgt gcggcaccga gaagtcgtag gtcagcccga
     1441 cgaagcccct ggccttggcc ttctcgaagg cgttggagag gtcggtcagg ttcacctgcg
     1501 agctaccgta ggtgacgatc ctggggctgg tgtggaacgc atagagcggt tccggtacca
     1561 tctcgaagtc cgtggcgctg ctgctcacgt ggtactcagg ggcgggcagg tcctcgtact
     1621 tgggcgaggt atcgagcagc ctggctgtag gctggttgtt ggtgggatcg tagacggtga
     1681 ttacttcgcc cttacgcccc acccagcggg tgtaaggcag ggccgaagac agccacctga
     1741 gcacgtcgtc cgtcgctgcc ggatcggtag cattagcgat cttgtagagc ttgtcggtgt
     1801 tggtccacca gttgccgtac cagggcgcgc tggtaatctg gcccgttacg gggtcgtact
     1861 gcggcctgga gtagaagtcg gtccagttgg acacggccag ccagttcacg tcgccgcgcg
     1921 gtatcgggcg atagagatag cccgaggtgg agcggaagaa gaggccgtag gccacgtcgc
     1981 ggcccaggcg aggcatcatg cggcagggag tgccgccggt gaactcgtac tgagggttca
     2041 ggtcgtcagc cgcgcacgct tcgccactct gctgccactg cgggtcggcc ttgtactgcc
     2101 agatgggcgg gtcgccgtgc gggtagtcgg aggacatagt gcgctcggca cctgcccagg
     2161 aggatgattc gtagtcgttg ggcgcggcac ccaggtcgat gaagtggaag cggtagcggc
     2221 cgccccaggt gcgggcgaag tccacgccgt cccaggcccc ggtgtcgggg ttggtaatgc
     2281 gccacgagcc gccctcttca accttcttgg acacgatcgc gttgttgatt accttgtcgg
     2341 tgccccaggt gtggtaagcg ttggggcgga agtactcgct cgcgtactcg ggcatgaagg
     2401 tatcgaagaa gaggaaagag gagcctttgt tggggccttg cggcatccgg tcgaggttca
     2461 gcccgttctt gtcgtagtag gggtcgtagt aagcgtcctc gcccggctgg atgacggccg
     2521 cgctatagag gtcgcccgtt tcgaggtcgc ggaaggcgca attccacagg tcgtcatagc
     2581 gcgagttgaa gatccagtcc tccatagcgt aggcatctat gaggtcggtc ttggtggggt
     2641 cggtcacctt ataggagggg tcgccgccct tggccaccac gcggtagtta ccgtagctct
     2701 gatcgtaacg cgcgaggctg gcaccgacct gtgccgtgtg gaacgactgg ttcggagctg
     2761 tgctgttctc ggcctgctgg aacaaggccc ttgcgtattc ttcggtggcc tgtaagaagc
     2821 gaatcttgta gttgtagcgg atgcccagga agtatggctt cccgttgatg tagtggttct
     2881 tgttccagtt caacaagccc gccgcctcgt tctgaatctc gtcggggatg ggggacttgc
     2941 tggaaacggt gggctggtag ttgtcgggta tccactgcct catttccgcg atttcttcag
     3001 cagtggggct gtaaccgacg aaaatgatgt tgagcgggta gtctaccgtc aacgcttcca
     3061 tgatgacgtc tctgctctcc ggatagacca ttccgggggg aagctgggta gattcgagcc
     3121 tggcgtagcc gttgtaggtg gtgccgaagg cggtatacgc cactacacgc acttcgtagg
     3181 tgccgccggg cagttcgggt agcgtcacct cttcgtaggt ggtggcccca gcagcgctgc
     3241 tgtcaacttc atcgccttct tcgttgtaaa catacatatc gaagtcggcc gagccggtcc
     3301 aggtaatgcc gatagtgacc ttaccttcgg cggtctccca gtaattgtcg gggagattga
     3361 cgttgagagt gaaggtatcg cagttggtgc tgctgtctga gcacagtgcg gggtcggggg
     3421 ttacgactgt ataaggcccg ccggtccagg taacctggct attagggggg ttgagagtgc
     3481 catccgcggg tgtagcagca tcgactaccc ctgcttgccc caacggctgc cctgccggtg
     3541 cgagggaaag caacatggac agcgtcacca gaatgcctat gatacgaact gatttcctca
     3601 ttcctgaaag ctcctcctgt ctgactcgcg gctcatcatc gagcacgcaa aaacggtgcg
     3661 gggcatgcta agatcgccgg ggaaaaacgt cctctcacat ctcagatcga ggtagaggta
     3721 tgggtgctct gtctgggcac agagggaaat tatgtttgca ttttccacag gatgccgctc
     3781 ggcaaaagcc tctttgaaag tgctgccccg caaatcggga agcttcagct aaatgaaatc
     3841 gcgtggaaaa cggcaacgga tggggcatag atcaagaatc gtgccctcat tgtgacatcg
     3901 aagcgtaccc aacccgacaa gggtttgtca aggccgagga tgcccagggc aggccgtgca
     3961 ggtgctgttc cagggctgtg acaaagtaag ctagcaaggc caaagcccca actgttccgt
     4021 ctgtgcccgg gtcagattgc ggcaccctaa cacccttagt cattctgagc gcagcgaaga
     4081 atct
//
LOCUS       DASRWZ010000117         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_558231, whole genome shotgun sequence.
ACCESSION   DASRWZ010000117 DASRWZ010000000
VERSION     DASRWZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558231"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="VFH60_01715"
     CDS             complement(<1..1486)
                     /locus_tag="VFH60_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312526.1"
                     /translation="MTKPKLPTAYMRVSLAFLLVLAAISAGVVGSGGKSALAAPLGVV
                     PAPSVMVPAGGTAYLDVRGFALQPGGSISPDALGPGTEPLGPAQLRTTLYYGINWGYT
                     DSEPQQVALALWYAQGSGWLDANHVTAERIASAAASSQGTPSWLPDGRSLLQLVASGD
                     VTVADLTLTPSALSAAMGTGTLAVHNTGTQDLLVHLPYGTTFTGASGSVLVWATGAGA
                     APPAATPVATAEPAENTPVPAVATDTPAPPTPTTGNTKSDFTPAPKATAPGGKSGKAS
                     PTSAASATPIPTDTPLPTDTPLPQPTETPTPEPTATDTPPTAVAEPPTNPIPDGGSKK
                     NTQPPASEGNPATGPANAQEPDKEKEPSGSAAPAQPVTEPDAEAQSPSKDVAMGPLPV
                     TPAATPTRPRPTQPAAPAPNGTSISSDQIPPPNGTSVSGQVPPPVTTAVGEPGGPVIP
                     QPQETAFLPVPTTPPTSAPETKPTELPTAGAGSDQAPGVEQKPTE"
     gene            1800..2798
                     /locus_tag="VFH60_01720"
     CDS             1800..2798
                     /locus_tag="VFH60_01720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET6312527.1"
                     /translation="MFDVRLPEITRDSGSDNYGRFRIEPLQPGWGTTIGASLRRILIS
                     SLVGAAVTGIRLSEMPDDPNEIAGIEEGLIDLVLNVKQLRFRVPADSQEAGESFRVAL
                     RFAGEETGEITASNLDLPEGVDLIDPDALIATATGQGVPFELELLVESGRGYGLAEVR
                     TDLPADMIPVDAMYTPVPRVNYVVEHTRVGQMTDYDRLLLDIWTDGTIEPDDALSQAA
                     QILTQYATAVARYGRDSSELDLEEAGPPEEDITNRPIEDLNLSMRTTNALRRANITTI
                     AQVLAMNDNDLLHLRNFGQKSLYELRDALAAQGFVMAVDMEGVEDEEGEEDELEEE"
BASE COUNT          517 a          808 c          996 g          585 t
ORIGIN      
        1 gctctgtggg cttctgttcc actcctgggg cctggtcgct gcccgcgcct gctgtgggca
       61 gctcggtggg cttagtctcc ggcgcgctgg tcggcggcgt tgtaggcacg ggcaggaaag
      121 cggtctcctg aggttggggt atcaccggcc cccctggctc accgacggct gtagtcacgg
      181 gcggcggcac ctggcccgac acggaagtgc cgttgggcgg agggatttga tccgatgata
      241 tggacgtgcc gttgggagcg ggcgctgccg gttgggtggg cctgggcctg gttggagtag
      301 ctgcgggcgt aacgggcagc ggtcccatcg ccacatcttt cgagggactc tgcgcctcgg
      361 catcaggctc cgtaaccggc tgggcggggg cggcgctgcc ggatggctct ttctctttat
      421 ccggctcctg tgcattcgca ggtcctgtcg ccggattgcc ctcagaagcg ggaggctgag
      481 tgttcttctt tgaaccgcca tccgggatag ggttggtcgg tggctccgct acggctgtgg
      541 gcggcgtatc ggtagccgtc ggctcaggcg tgggcgtctc agttggctgc gggagcggag
      601 tgtccgtcgg taggggagta tcagttggga tgggagttgc tgaggcggcg cttgtaggcg
      661 atgccttgcc cgatttgccg ccgggcgcgg tcgccttcgg cgcgggggta aagtcgctct
      721 tggtgttgcc ggtggtcggc gttggcggcg cgggcgtatc tgtagcgaca gccggtaccg
      781 gagtattctc ggcgggttcg gcggtggcta ccggcgtggc ggcaggggga gccgcgcccg
      841 caccagtggc ccaaacgagc actgagccgg atgccccggt aaaggtcgtg ccgtatggca
      901 ggtgaaccag caggtcttgc gtgcccgtgt tatgcacggc cagggtgcct gtgcccatag
      961 ccgccgacag cgcgctcgga gtgagcgtga ggtccgcgac cgtgacatca ccggaggcga
     1021 ccagttggag gagcgagcgg ccatcgggta accagcttgg cgtaccctgt gacgaggcag
     1081 ccgcgcttgc gatgcgctcg gctgtgacat ggttggcgtc gagccagccg ctcccctgcg
     1141 cgtaccagag cgccagggcg acctgctgcg gctcgctgtc ggtgtagccc cagtttatgc
     1201 cgtagtagag ggtagttcgg agttgcgccg ggcccagcgg ctctgtgcca ggccccaacg
     1261 catcggggga gatagacccg cccggttgga gcgcgaaacc tcttacgtcc aggtaggccg
     1321 tgccgcccgc cgggaccatt accgatggcg cgggcacgac gccgagtggc gctgccaggg
     1381 cggatttccc gcccgaaccg accacgcccg ccgaaatggc cgccaggaca agtagaaatg
     1441 caaggcttac acgcatgtaa gcggtgggga gcttaggctt agtcaaatta gcgagccgcc
     1501 tttcactgtg cgaggattac ttcgagagta tacaatttca cgcaacaact ttgcaagttc
     1561 acgacgacga tttctaaccg ccaagatgcc aagagtttta tggtttggtt ttcagtacaa
     1621 tgccttgagc gcgaactaaa cattcctatc aatcttaacg tcttggcgtc ttggcggtta
     1681 gctcctgatt accatcataa aaggccctgt gaaatttgcg attcggggcc tcttatgata
     1741 tagtataagg tcagtttttg tctggctttt ctgttgtatc taaaatgggg gtgagttagt
     1801 tgtttgatgt aaggttgcct gagataacca gggatagcgg tagcgacaat tatggcaggt
     1861 tccgcattga gccgctccag ccgggctggg gcactactat aggcgctagc ttgaggcgca
     1921 tcctgatttc gtccctggtg ggcgcggcag tcaccggcat ccgcctctcc gagatgcccg
     1981 atgaccctaa cgagatagcg ggcatcgaag agggccttat tgacctggtg ctgaacgtga
     2041 agcagttgcg cttccgggtg cccgccgaca gccaggaagc gggagagagt ttccgtgtgg
     2101 cgttacgctt cgcgggcgag gagacgggcg agatcaccgc gagcaacctc gacttgccgg
     2161 aaggcgttga tttgatagac cccgatgccc tgattgccac cgctacgggg cagggcgtgc
     2221 catttgagtt ggagttgctg gtcgagtcgg ggcgcggtta cggattggct gaagtgcgta
     2281 ccgacctgcc cgccgatatg ataccggtag acgctatgta tactccggtg ccccgcgtaa
     2341 actacgtggt ggagcacacc cgcgtaggcc agatgaccga ttacgaccgc ttgctgctgg
     2401 acatctggac ggacggcacc atcgagccgg acgacgccct cagccaggcc gcgcagatac
     2461 tcacccagta cgcgaccgcg gtggcgcgct acgggcggga tagctcggag ctagaccttg
     2521 aagaggcggg gccgccggaa gaagacatca cgaaccgccc aatcgaagac ctgaacctca
     2581 gtatgcgtac caccaacgct ctgaggcgcg ccaacatcac cacaattgcg caggtgctcg
     2641 ccatgaacga caacgacctg ctccacttga ggaacttcgg ccagaagtcc ctctacgagt
     2701 tgcgggacgc gctggcggcg cagggtttcg tcatggccgt tgatatggaa ggcgtagaag
     2761 acgaagaggg cgaagaggac gaattagaag aagaataggc gtgctgcgtt ttgtcgcatg
     2821 ggaggtggct aggccacctc ctttttcatt ggccgtaaac tacctcgaag atatagaccc
     2881 cgccctggaa gtagagtagc cggtag
//
LOCUS       DASRWZ010000118         1912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_489511, whole genome shotgun sequence.
ACCESSION   DASRWZ010000118 DASRWZ010000000
VERSION     DASRWZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489511"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..416)
                     /locus_tag="VFH60_01725"
     CDS             complement(111..416)
                     /locus_tag="VFH60_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET6312528.1"
                     /translation="MDARMAKQLQQMQAKMVKAQEELANETVTATAGGGAVTVEMNGH
                     HEVVSVKLDPAAVDPDDVEMLQDMIVAALSDALQKAQDLAQRKMSAITGGLNIPGLF"
     gene            complement(433..>1912)
                     /locus_tag="VFH60_01730"
     CDS             complement(433..>1912)
                     /locus_tag="VFH60_01730"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312529.1"
                     /translation="EPPPQTAFILATTEVHKVPATVASRCQRFDFRKIPLSAMTARLR
                     HVCDEEGIEAEQAALELVARQSTGSLRDALSLLDQLRVYAEEGITLKSVQDMLGTSGA
                     EQVADFVDTMLAGDLGAGMQTVNRLLDEGLDARQFNRQIVEHLRNLMLVKSGAASDTA
                     LLDATEEMRARLNAQAAKSSMPELLRWVKEFAEADGAIRSSAYRQLPLEMALVSALLP
                     PEAEARAQVRERGAELASAIEPIAAVPPTRPNPARQAPPPAPEPQVQMPPPIHRELGA
                     LSSNGSSNGNGNSPRTNPDTQPRPAAPAPIEPPAYLYEGVEDAVESAPRGMAQMGGGY
                     DGPPLPPAPPGNGDAATGDVGSGDHLERLRQLWPRLCDQISARSRPIGSAFRNPALVR
                     PFAVKGSRVIISFRDPIQRTRSQGEPHRSIIEESLIRVLGHKCVLESILFSEEETGGP
                     PTEPFSAEMRGRAAPKEKKTAPHETTRGRAAMNIFGITKFDD"
BASE COUNT          294 a          644 c          601 g          373 t
ORIGIN      
        1 aaggaggcga aaagaaaaat cccacgggaa tatccttaag tgggaccttt ctttttaggg
       61 tacattgacg agctatcgtc actcgtcact cgtcactcgt cactcgtcac ttagaacagc
      121 cccggaatgt tcagcccgcc cgtaatggcc gacatcttcc tctgggccag gtcctgggct
      181 ttctgcaagg cgtcactcaa cgcggcgaca atcatgtcct gcaacatctc cacgtcgtcc
      241 gggtccaccg ccgccgggtc gagcttcacg ctgacaacct cgtggtggcc gttcatctcc
      301 accgtcaccg caccgccgcc cgccgtggcc gtgacggtct cgttcgcaag ctcttcctgg
      361 gctttcacca tcttggcctg catttgctgc aattgctttg ccattctagc gtccattcgc
      421 aactcctcta gactaatcgt caaacttggt aatgccgaag atattcatcg ccgcccggcc
      481 acgggttgtc tcgtgggggg ccgtcttctt ctctttgggc gcggcccttc ctctcatctc
      541 ggccgaaaag ggttcggtgg gcgggccacc ggtctcctcc tccgagaaca ggatgctctc
      601 caatacacac ttatggccca gcacgcggat aagcgactcc tcgataatgc tgcggtgcgg
      661 ttccccctgg ctgcgggtgc gctggatggg gtcgcggaag ctgattatca cgcggctgcc
      721 cttcacagcg aacggcctca ccagcgccgg gtttcggaac gcgctgccaa tggggcggct
      781 tctcgcgcta atctgatcgc acaacctggg ccacagttgc cgcaagcgct caaggtggtc
      841 gccgctaccc acatcgccgg tagccgcgtc gccgttgccg ggcggggccg ggggcagcgg
      901 cgggccatca tagccgcccc ccatctgtgc cataccccgc ggggcgctct ctaccgcgtc
      961 ctccacgcct tcgtacagat aggcaggcgg ctctatcggg gcgggggccg cgggcctcgg
     1021 ctgggtatcg gggttggtcc ggggcgagtt accgttgccg ttgctggaac cgttgctgga
     1081 tagggcacca agctccctgt gaataggtgg gggcatttgt acctgcggct caggagcggg
     1141 gggcggtgcc tgcctcgcgg ggttgggcct cgtgggcggc acggcggcga tgggttcgat
     1201 tgcagaagct agctcggccc cgcgctcgcg tacctgcgcc cttgcctcgg cctctggcgg
     1261 cagcagcgcc gacaccaacg ccatctccag cggcaactgg cggtaggccg acgaccgtat
     1321 agccccatcg gcctcggcaa actccttcac ccagcgcagt agctccggca tcgaggactt
     1381 cgcggcctgg gcgttcaggc gagcgcgcat ctcctcggtg gcgtctagca gggcggtatc
     1441 cgaggcggcc ccgctcttca ccagcatcag gttgcgcagg tgctccacta tctggcggtt
     1501 gaactgccgg gcgtccagcc cttcatcgag caagcggttg accgtctgca tcccggcccc
     1561 caggtcgccc gcaagcatcg tgtccacgaa gtcggccacc tgctccgcgc ctgacgtgcc
     1621 aagcatgtcc tgcacgctct tgagggtgat gccctcctcc gcgtacaccc ttagctggtc
     1681 gagcagcgag agcgcgtcgc gaagggagcc ggtggactgg cgggcgacaa gctccagcgc
     1741 cgcctgttcc gcctcgatgc cctcctcgtc gcaaacgtgg cgcaggcggg cggtcatagc
     1801 gctcagcggt atcttgcgga agtcgaaccg ctggcagcgg gaggccaccg tggccgggac
     1861 cttgtgcacc tcggtggtag cgaggatgaa agcggtctgc ggcggcggct ct
//
LOCUS       DASRWZ010000119         4773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_900951, whole genome shotgun sequence.
ACCESSION   DASRWZ010000119 DASRWZ010000000
VERSION     DASRWZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900951"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1362
                     /locus_tag="VFH60_01735"
     CDS             166..1362
                     /locus_tag="VFH60_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6312530.1"
                     /translation="MRVGINAHLLSFSGSYRQAGLSRYIDELLFELPRVAPDLQITGF
                     TGNEALPASARARQPSNLTLAPSRFPTQRAPVRIAWEQAVLPGAALRHRLDLLHCPVN
                     VRPILSPCPTVVTIHDLVFLRSPQSFHPAKRRYLSAMTGWSARHASHVIAVSDSTRQD
                     VIDLLGVRPNRVTTVHNGVGEQFKPLDAAERSAFRAQQGIGGRTILYVGTLEPRKNLP
                     MLIRAFARLSEDPAFEDVKLYIGGSKGWYYDEIFATAERLGLTQSGRVTFLGRVPDEQ
                     LPLWYNVATVVAYPSLYEGFGLPALEAMSCGTPVLASNASAMPEVVGGGGLLLDPTDD
                     AAWLDALRAVLSDDALRADLAGEALKQAAAFSWTRSARETAEVYRRVQNRRGSKQSER
                     VGARRR"
     gene            1359..1865
                     /gene="tsaE"
                     /locus_tag="VFH60_01740"
     CDS             1359..1865
                     /gene="tsaE"
                     /locus_tag="VFH60_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET6312531.1"
                     /translation="MIKGELPAVADLDIVSHGADQTRRLGEHLGRSLQPGDLLLLEGD
                     FGSGKTTFTQGIAQGLGIDARYVNSPTFTLINEYDGGRVHLAHIDLYRLEGDEQVATL
                     GLDDYLAGHGVTVIEWPLGAAAWLPDEYMLVRFSHLSETKRTLRFWALGPRYRELAEL
                     FKRDAYGA"
     gene            1912..2604
                     /gene="tsaB"
                     /locus_tag="VFH60_01745"
     CDS             1912..2604
                     /gene="tsaB"
                     /locus_tag="VFH60_01745"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET6312532.1"
                     /translation="MILALDTSTEYASIALYLPGQGVIAEQTWRSGREQTTQLLPAVE
                     SLMSLVAVETGGLAGVAVATGPGSYSGVRIGISTAKTLAFSLGIKLWGVPSLDVLAYS
                     QVAVTAAQVCAVLSLGRGRYGWALYRTRGARWQRRNADANNTLPEIASQAAEAGTEAR
                     TVTMFCGELDAAAAAQITEIMGNNASIAPAAGGLRRAGYLAELALQRSERGETDDPAT
                     LQAIYLQPKAEA"
     gene            2695..3468
                     /gene="rimI"
                     /locus_tag="VFH60_01750"
     CDS             2695..3468
                     /gene="rimI"
                     /locus_tag="VFH60_01750"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET6312533.1"
                     /translation="MPYILEYMTHDDIPQVAAIERVCFSMPWPVSAYKRELKTPETNR
                     YIVVRFLTPEQARQVNLPGPTPANLSRLTHPELLSAGDEVALSRPQSVGGLSRLTSLL
                     PWRKNGHSEAVEEVTHRQFPVVGYAGLWLMVDEGHVTTIGVHPDHRGKGAGELLFLGL
                     VDIAAEMKAARVTLEVRVSNLPAQALYRKYGLEIAGVRKRYYSDNGEDAYIMWSEPVG
                     SPHFEERMRQLREDLAARFRASFDTDLPAARPVDRSRLQ"
     gene            3480..4577
                     /gene="tsaD"
                     /locus_tag="VFH60_01755"
     CDS             3480..4577
                     /gene="tsaD"
                     /locus_tag="VFH60_01755"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET6312534.1"
                     /translation="MNILAIETSCDETSVALVRNGRHILANVIASQIPLHQKYGGVVP
                     ELASRQHVVTIVPVLEEAMKRANLDWPQVDAVAVTKGPGLSPALLVGVNAAKALAFAR
                     HKPLLGVNHMEGHIYSNWLIPEAAAGDPPLEPHFPLLNLIVSGGHTELVLMTGHGQYR
                     MLGKTIDDAAGEAFDKAARILGLGYPGGPAIQRASEGGDPARFQLPRAWLKGTYDFSF
                     SGLKTALLRKVQEYGVVASKPAPWQKQEAEGAAPPSPVAAVPIVERELPVADLAASFQ
                     AALVDALVEKTAAAAAEYGVREVLVAGGVAANASLRSRLKERLPIPFRYPPIVLCTDN
                     AAMIAAAAHYRYEDALQHDLSMDIEPSARYV"
BASE COUNT          864 a         1712 c         1411 g          786 t
ORIGIN      
        1 gagcggtggg ttagccgaaa agtactctcc ggcgcgctga aaggagtata cgataggtat
       61 tatgtcatgt caataggtcg taggagaaaa gtactacgcc acacgccttc ctacgcacga
      121 aatgccctcc atgcagcccc tgccctccgc gggtataatg tcagcatgcg cgtcggcatc
      181 aatgcacacc tactctcctt cagcggcagc taccgccagg cgggactcag ccgctacata
      241 gacgaactgc tattcgagct gccccgcgtc gcccccgacc tacaaatcac cggctttacc
      301 ggcaacgagg ccctacctgc ctccgcacgc gcccgccaac cgtcgaacct caccctcgcc
      361 cccagccgct tccccaccca acgcgccccc gtccgtatcg cctgggagca ggccgtccta
      421 cccggcgcgg ccctccggca caggctcgac ctcctccact gccccgtgaa cgtccgacct
      481 atcctgtcgc cctgccccac cgtggtcacg atacacgacc tggtgttcct gcgctccccc
      541 caaagcttcc accccgccaa gcgccgctac ctgagcgcca tgaccggctg gtcggcccgt
      601 cacgcctccc acgtgatcgc cgtctccgac tccacccgcc aggacgtgat cgacctgctg
      661 ggcgttcgcc ccaacagagt gaccaccgtc cacaatggcg tgggcgagca gttcaagccc
      721 ctcgacgcag ccgagcggtc cgccttccgg gcgcaacagg gtattggagg ccgaaccata
      781 ctctacgtgg gcacgctgga gccgcgcaag aacctcccca tgctcatccg ggctttcgca
      841 cgcctctcgg aagaccctgc cttcgaggac gtgaagctct acatcggggg cagcaagggc
      901 tggtactacg acgagatatt cgccaccgcc gagcgcctgg gtctcaccca atcgggccgc
      961 gtgaccttcc tggggcgcgt gcccgatgag cagctaccct tgtggtataa tgtcgccacc
     1021 gtggtcgcct atccttcctt atacgagggg tttggcctgc ctgccctcga agcaatgtcc
     1081 tgcggcacgc ccgtgctcgc ctccaacgcc tccgccatgc ccgaggtggt aggcggcggc
     1141 ggcttgctgc tcgatcccac agacgacgct gcctggctcg acgcgttgcg ggcggtgctc
     1201 tccgacgacg cactgcgggc ggaccttgcc ggcgaggcgc tgaagcaggc ggcggcgttc
     1261 agttggaccc gctcggcccg cgagacggcg gaggtctacc ggcgggtgca aaacaggcgg
     1321 gggagtaagc aatctgagag ggtgggggcg agacggcgtt gatcaagggc gagcttccgg
     1381 cagtcgcaga cctcgatata gtgagccacg gggccgacca gacgcgccgc ctcggggagc
     1441 acctgggcag gtccctccaa cctggcgacc tgctgctcct ggaaggcgat ttcggctccg
     1501 gcaagactac cttcacgcag ggcatagccc agggcctcgg catcgacgcc cgctacgtca
     1561 actcacccac ctttaccctc atcaacgagt acgacggcgg ccgcgtccat ctcgcccaca
     1621 tcgacctcta caggctcgaa ggagacgaac aggtcgccac gctgggcctg gacgactacc
     1681 tggcaggcca cggcgtcacg gtcatcgagt ggcccctggg cgctgccgcc tggctccccg
     1741 acgagtacat gctggtccgc ttctcgcacc tgagcgagac caagcgcacc ctccgcttct
     1801 gggcgctcgg cccgaggtac cgcgagctgg ccgagctatt caagcgagac gcctacggtg
     1861 cctgacgcaa tttcctggcc ggtctagcca ctacctctta cgacgccaac catgatactt
     1921 gccctcgaca catccacaga gtacgccagc atagccctct accttccggg tcagggcgta
     1981 atcgccgaac agacctggcg ctccggccgc gagcagacca cccaactgct gcccgctgta
     2041 gagagcctca tgtcactggt ggccgtcgag acgggcgggt tagccggtgt ggcggtggct
     2101 accgggccgg ggtcgtacag cggggtgcgc atcggcatca gcaccgccaa gacgctggcc
     2161 ttctccctcg gcataaagct ctggggcgtg ccgagcctcg acgtgctggc ctactcgcag
     2221 gtggccgtca ccgccgccca ggtctgcgcc gtgctgtccc tgggcagggg ccgctacggt
     2281 tgggcgctct accgcactcg cggggcgcgc tggcagcgac gcaacgctga cgccaacaat
     2341 acgctccctg agatagcctc ccaggccgcc gaggccggca cggaagcccg caccgtcacc
     2401 atgttctgcg gcgagctgga cgcggcggcc gccgcccaaa tcaccgaaat tatgggcaac
     2461 aacgcctcta tagctcccgc cgccgggggc ctgcgccggg ccggttacct ggccgagctt
     2521 gccctccaac gctccgagcg gggggaaacc gacgaccccg ccaccttaca ggctatctac
     2581 ctgcaaccaa aggccgaagc ctgacctgcc gttcctgtct aaagcgtgta tacttgaaca
     2641 tcatggaccg cgcggagtgg cgcaagctcg atgaggagca ggtgctgaga tagaatgccc
     2701 tacatattag agtatatgac ccacgacgac atcccccagg tcgcggccat cgagcgcgtc
     2761 tgcttcagca tgccctggcc cgtgtctgcc tacaagcggg aattgaagac gccggaaacg
     2821 aaccgctaca tcgtcgtgcg ctttctcacc cctgagcagg cccgccaggt aaatctcccg
     2881 gggccgacgc ccgcgaatct cagccgcctg acccatcccg agttgcttag cgccggtgat
     2941 gaagttgccc tctctcggcc tcaatcggtg gggggcctct cgcgtcttac ctccctgctg
     3001 ccctggcgca agaacgggca ctccgaggca gtggaagaag tgacccacag gcagttcccc
     3061 gtcgtcggtt acgcgggcct ctggctcatg gtggacgagg ggcacgtcac cacgataggc
     3121 gttcaccccg accacagggg caagggcgct ggtgagctgc tcttcctggg gctggtggac
     3181 atcgccgccg agatgaaggc cgcccgcgtg acattggagg tgcgcgtctc gaacctgccc
     3241 gcccaggcac tctaccgcaa gtacggcctg gaaatcgccg gggtccgcaa acgctactac
     3301 agcgacaacg gcgaggacgc ttacatcatg tggagcgagc cggtcggctc cccacacttc
     3361 gaggaacgca tgaggcagtt gcgcgaggac ctggcggcac gctttcgcgc cagcttcgat
     3421 accgacctgc ccgccgcccg cccggtggac cgctcccggc tacagtagat ctaacgccca
     3481 tgaatattct tgctatcgaa acttcctgcg atgagacctc cgtcgccctc gtgcgcaacg
     3541 gcaggcacat actcgccaac gtaatcgcct cgcagatccc cctgcaccag aagtacggcg
     3601 gggtcgtgcc ggagcttgcc tcccgccagc acgtggtcac catcgtgccc gtgctcgaag
     3661 aagcgatgaa gagagcaaac ctggactggc cccaggtgga cgccgtcgcg gtgaccaaag
     3721 gccccggcct ctcgcccgcc ctcctcgtgg gggtaaacgc cgccaaggcc ctggccttcg
     3781 cccggcacaa gcccctgctc ggcgtgaacc atatggaagg ccacatctac tccaactggc
     3841 tcattcccga agccgcagcc ggggaccccc cgctggagcc gcactttcct ttgctcaatc
     3901 tcatcgtttc cggtgggcat acggagcttg tgctcatgac cggccacggc cagtaccgca
     3961 tgctgggcaa aacgatagac gacgcggcgg gggaagcttt cgataaggcc gcccgcatcc
     4021 tgggtctcgg ctaccccggc ggccccgcca tacaacgcgc ctctgaaggt ggcgaccccg
     4081 cccgcttcca actgccccgc gcctggctga aaggcaccta tgacttctcg ttcagcggcc
     4141 tcaagaccgc gctcttgcgc aaggtgcagg agtacggcgt ggtcgccagc aagcccgcgc
     4201 cctggcagaa gcaggaggcc gagggggccg ctcccccttc gcccgtagcg gccgtgccca
     4261 tcgtggagcg agagctaccc gtggccgacc tcgctgcctc cttccaggca gcgctggtgg
     4321 acgccctggt agaaaagacg gcggccgcgg ctgctgagta cggcgtccgc gaagtgctgg
     4381 tggccggtgg cgtggcggcc aacgcctccc tccgctctcg cctgaaggag cgcctgccca
     4441 tcccgttccg ctaccctcca attgtcctct gcaccgataa cgccgccatg attgccgccg
     4501 ccgcccacta ccgctacgag gatgccctgc aacacgacct ctcgatggac atcgagccaa
     4561 gcgcccgcta cgtgtgacag ggtgtaaagg gacggaaccg ccaagacgcc aagaacgcca
     4621 agaaccacgc caagaaccac gccaaggtat caaggttttg agagtacaaa atgaaccagg
     4681 gtacaacatt ctgtaacctg gtccttctgc ttagaacact tttatcaggc tctgcgtaac
     4741 ctctgcgctc tctgcgtctc tgcggtgaac cgt
//
LOCUS       DASRWZ010000120         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_78525,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000120 DASRWZ010000000
VERSION     DASRWZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78525"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..>2655
                     /locus_tag="VFH60_01760"
     CDS             331..>2655
                     /locus_tag="VFH60_01760"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HET6312535.1"
                     /translation="MNNNRRPIVRGGGRVRRERYDDRPDPYLLDAEIARNRPATDGLT
                     WKKMDLHIHTPASSDYRDPGVTYLDILKKAEEKGLDMIAFADHNTVSGYAAMHQEIET
                     LTLLERLGRISDDEQATLDEYRRLLKKIVVLPAFEFTATFGFHILGVFPEGTSIRKLE
                     YLLLNLNVPEDKMVRGAPDVGSSDDVLTAYAAIAGAGGMAIAAHANSSNGVAMQGFPF
                     GGQTKIAYTQDPNLVALEVTDLDQTGRRATAGFYNGTKTEYPRRMHCIQGSDAHSLET
                     EQSDANNKRLGVGARITEILVREASFAALREVLMGDDYTRTRPYRSNPAWERIEAARA
                     KGPNQIVDFHERAVHVHNRTRPILEDVVAFANGYGGIIYIGASPDTKVPVQGVERPEE
                     VVRLLREDVQRMVEPRLDVNFDIKPSSTSDRAIVVMNVPGGDNRPYVFAPTTQIYIRE
                     GGESLLATRAEMIKLIVDAARARGELAPVASTTTHQPITTPAATASPVPAVPEPRPIL
                     PAGEPLARSDDAQPTPQAQERRQGRQQDRQGRQREERQEQPAAREQRKPWREQGDDRR
                     AAREAAETPAAEVESPEETPAQPTAPLREERRAAAPAADAAPVPVPTRPESQPGRRIP
                     SLHELPPEKIKGQVQPMARTGGSPAPTPESQATEPPQSALYAVQGADAQEVAPEVERA
                     IIEAASTASDAPDVAPPVIEGTERLEVGYEPTAPARGRGTRSRRKPGSAIPEQATREM
                     PAATVSETAEQAAEAQPAQVEQAEQAEQPEEAAPA"
BASE COUNT          554 a          924 c          817 g          360 t
ORIGIN      
        1 cgggcaggcg caagaaccag ggccacgtgc cctctccctc ccccagcgtc cagacccagc
       61 ccagcgccaa ggccgcacag aaaaaaccta agtaattctg gattttagat tttggattaa
      121 gaagaattca gagcaaatct aaaatccaaa atctaaaatc taaaatccaa ccaccagcct
      181 cgccccatta tttacataga cgcaccgatc ttccaacctg gattgcagtc ttgcgaacgg
      241 cagcatgccg ctcgtgattt gcgcgcgttc cggggtgtgt agcttctagc tacgcgaaga
      301 gtgggcgtct caccacagat tgaggcatga atgaacaaca acagacgacc cattgtgcgc
      361 ggcggaggcc gcgtcaggcg cgagcgttac gacgacaggc ccgaccccta tttgctggac
      421 gccgagattg ctcgcaaccg cccggccacc gacggcctga cgtggaagaa gatggacctc
      481 catatccaca cgcccgcctc ctccgattac cgcgacccag gcgtgacgta cctggacata
      541 cttaaaaagg ccgaagagaa gggcctagat atgatcgcct tcgccgacca caacaccgtg
      601 tccggctacg cggcgatgca ccaggagata gagaccttga ccctcctgga gcgcctgggc
      661 cgcatcagcg acgacgagca ggccaccctc gacgagtacc ggcggctcct caagaaaatt
      721 gtcgtgctgc ccgcgttcga gtttacggcc acgttcggct ttcacatcct aggcgtcttc
      781 cccgaaggca cctccatccg caagctggaa tacctgctgc tcaacctcaa cgtgccggag
      841 gacaagatgg tgcgcggcgc gccggacgta ggctccagcg acgacgtgct caccgcctat
      901 gcggctatag ccggggcggg cggcatggca atcgccgcgc acgccaactc ctccaacggc
      961 gtggctatgc agggcttccc cttcggcggg cagaccaaga tcgcctatac ccaggacccc
     1021 aacctcgtcg ccctcgaagt gaccgacctc gaccagaccg gcaggcgagc cacggcgggc
     1081 ttctacaacg gcaccaagac cgagtacccc cgccgcatgc actgcatcca gggttccgac
     1141 gctcactcgc tggagaccga gcagtcggac gccaacaaca agcgcctcgg ggtaggggcg
     1201 cgcatcaccg agatactggt gcgcgaggcc tccttcgcgg ccctacgaga ggtgctgatg
     1261 ggcgacgact acacgcgcac tcggccctac aggtccaacc ctgcgtggga gcgcatagaa
     1321 gcggcgcggg ccaaggggcc taaccagatc gtggacttcc acgagcgggc cgtgcacgtc
     1381 cacaaccgca cccgccccat cctggaggac gtggtagcgt tcgccaacgg ctacgggggt
     1441 atcatctata tcggtgcgtc tcccgacacc aaggtgcccg tgcagggcgt ggagcgtccg
     1501 gaggaggtcg tgcgcctgct cagggaggat gtgcagcgta tggtggagcc tcgccttgac
     1561 gtgaatttcg acatcaagcc aagctctacc agcgaccgcg ctatcgtggt gatgaacgtg
     1621 ccgggcggcg acaaccgtcc ctacgtattc gcgcccacga cccagatata catccgcgaa
     1681 ggcggggaga gcttgcttgc cacccgcgcc gagatgataa agctgatcgt ggacgcggca
     1741 cgagcgcgag gcgaacttgc gcccgttgcc tccacgacta cccaccagcc aatcaccacc
     1801 cccgccgcga ccgcctcacc cgtgcccgcc gtgccggagc cacgtcccat cctgcccgcg
     1861 ggcgagccgc tggcacgctc ggacgacgca cagcccacac cgcaggccca ggagcgcagg
     1921 caagggaggc aacaggaccg ccagggcaga caacgggaag agcggcagga gcaacctgct
     1981 gcccgcgagc agcgcaagcc gtggcgcgag cagggcgacg accgccgggc cgcgcgtgag
     2041 gccgctgaga cccccgccgc cgaagttgaa agcccggagg aaacccccgc acagcccaca
     2101 gcaccgttgc gggaagagcg tcgcgcggcc gccccggctg ccgatgccgc gccggtgcct
     2161 gtgcccaccc gcccggagag ccagcccggt aggcgcatac cctccctgca cgagcttccg
     2221 cctgagaaga tcaaggggca ggtacagcct atggcccgca caggtgggtc gcccgcaccc
     2281 actcccgagt ctcaggcaac ggagccgcca cagtccgcct tgtacgcggt acagggggcc
     2341 gacgcccagg aagtggcccc tgaggtggag cgggcgataa tcgaggcggc gtccactgcg
     2401 tccgacgcgc cggatgtcgc cccgcccgtt atcgagggta cggagcgcct ggaggtgggg
     2461 tacgagccta cggcccccgc aagaggccga ggcacccgaa gccgccgcaa gccggggtcg
     2521 gccataccgg agcaggccac cagagagatg cctgccgcca cggtgagcga gacagccgag
     2581 caagcagccg aagcccagcc tgcccaggtc gagcaagctg agcaagctga gcagcctgag
     2641 gaagccgctc ctgcc
//
LOCUS       DASRWZ010000121         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_147588, whole genome shotgun sequence.
ACCESSION   DASRWZ010000121 DASRWZ010000000
VERSION     DASRWZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147588"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..2641
                     /locus_tag="VFH60_01765"
     CDS             212..2641
                     /locus_tag="VFH60_01765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HET6312536.1"
                     /translation="MSSTFTKNSANSKRVALDLEAELLRLFGYGEFRGRQKEIISQVL
                     QGTDTIAVMPTGAGKSLCYQLPAMLLPGTTLIISPLIALMKDQYDNLPSDVYERTTFI
                     NSSLDAEELGQRMSEIIAGKYKLVYCAPERLRQQQFTEALRRARISMLVVDEAHCVSR
                     WGHDFRPDYLFIGKCLPQLGHPTLFALTATATPEMREEIGEQLGRKLKPIVASVFRPN
                     LFYEVEELNDKEAKLKRLVEICQREHGAGVIYGRSREICEQIAAMLRRSGVKAGFYHA
                     GMESEARAAAQEAFMLDRTRVIVATIAFGMGVDKSNVRFIVHFSPPDSLEGYVQESGR
                     AGRDGRPARCILMMTPGDKSNLSRWQREEQMKIEPLRAIYKELSRHVPVGGARFLRFE
                     EIERDATNSGTPVDSMQSRVGVSMLERVGLIVRHPDAPKAMYLTLLPEGVAAKDPQFR
                     GFVTTAGLVQGQPTRRDTAALAEAMQVTAPELERLALVWEEYGWLRARGERRDPVIER
                     LRPPADTAQTIQRLLDEQERNQQHQIDQIMRYATASRCRHQMLAAHLGEQIDECGTSC
                     DYCAPPADRPDDVQAQAAELPDNPGQIIIACLMSFPFQAGKPSLVKALTGSAASNVKA
                     DRVRHFGALAAASPSAIMKAIDELVEGGYLAFYEHDEGFKLLRVTEEGLEGVPANAVS
                     LKQKRQPKPRDRAGGAVTPRPDRAARVDTERTKAVRRSEEDREPTPEEADLFERLRQW
                     RRVIANRLGLPPYIIFHDKTLWAIAKAQPTTVEDLLQIKGVGQSHIDKYGDELLEALA
                     SFAETEDELES"
     gene            complement(2803..3237)
                     /locus_tag="VFH60_01770"
     CDS             complement(2803..3237)
                     /locus_tag="VFH60_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312537.1"
                     /translation="MENTETTVDLELDKAALLKIHAHDRQAHFDTDVDLLLAHSGHEF
                     ISVSNGEVHRSTLAEQHAFFEGYFKGATYYEWDDLEPPIIRISADASMAWMIVRTQVR
                     RATRLEGAEEAREVRFVYAGIMTYEKNAGNWVRVANVSTFAS"
BASE COUNT          672 a         1082 c          999 g          540 t
ORIGIN      
        1 cgcggagttt accctgagcg tagcgaaggg ctcagaatga ccaaggctga taacgaccct
       61 gatttcgacc ggcctcagtg cctcgctttg tgtgagaaat gcactcaaag ctgctcacct
      121 gtggtacaat tagatagtac aaagagacgt tgccttcggc ttcagaatcc cggcttcggg
      181 gcatttccca ctagcaagtg ccaggctctc catgtcctca actttcacca agaacagtgc
      241 caacagcaag cgtgtcgccc tcgacctcga agccgagcta ttgcgactct tcggctacgg
      301 cgagttccgg ggcaggcaaa aagagatcat ttcgcaggtg ctacagggca ccgacacgat
      361 agccgtcatg cccaccggcg cgggcaagtc cctctgctac caactcccgg ccatgctcct
      421 gccgggcacc acgctcatca tctcgcccct catcgccctg atgaaggacc agtacgataa
      481 cctcccctcc gacgtctacg agcgcaccac cttcatcaac agcagtctcg acgcggagga
      541 gcttgggcag cgcatgtccg agatcatcgc gggcaagtac aagctggtgt actgcgcgcc
      601 ggagcgcctg aggcagcagc aattcaccga ggcgttgcgc cgggcgcgta tctccatgct
      661 ggtggtggac gaggcgcact gcgtctccag gtggggccac gacttccgcc ccgactacct
      721 gttcataggc aagtgcctgc cccaactcgg ccaccccacc ctgtttgccc tcaccgccac
      781 ggccacgccg gagatgcgcg aggagatagg cgagcagcta ggccgcaagc tcaagcccat
      841 cgtggcgagc gtcttccgcc ccaacctctt ttacgaagtg gaggaactga acgacaaaga
      901 agccaagctg aagcggctgg tggagatttg ccagcgcgag cacggggccg gggtaatcta
      961 cgggcgcagc cgcgagattt gcgagcagat tgccgctatg ctccgtcgct ccggcgtgaa
     1021 ggcgggcttc taccacgcgg gcatggagtc ggaggcccgc gctgccgccc aggaagcgtt
     1081 catgctcgac cgcacgcgcg tgatagttgc gaccatcgcg ttcggcatgg gcgtggacaa
     1141 gagcaacgtg cggttcatcg tgcacttcag ccccccggac tcgctcgaag gctacgtgca
     1201 ggagtcgggc cgggcgggcc gcgacggtcg ccccgcgcgc tgcatcctca tgatgacccc
     1261 aggcgacaag tccaacctga gtcgctggca gcgcgaagag cagatgaaga tcgagcctct
     1321 acgcgccatc tacaaggagt tgagcaggca cgtcccggtg ggcggggcgc ggttcctgcg
     1381 cttcgaggaa atagagcggg acgccacaaa ttccggcacg cccgtggaca gcatgcagtc
     1441 gcgcgtgggc gtgagcatgc tggagcgcgt cggcctgatc gtgcgccacc ccgacgcgcc
     1501 caaggccatg tacctgaccc tgctccccga aggtgtcgcg gcgaaagacc cccagttccg
     1561 cggcttcgtc accactgccg gcctggtaca gggccagccc acccgccgag acaccgctgc
     1621 cctcgctgag gcgatgcaag tcaccgcgcc cgagctggag cggctggccc tggtatggga
     1681 ggagtacggc tggctgaggg cgagaggcga gcggcgcgac ccggtaatcg agcgactgcg
     1741 gccccccgcc gacaccgccc aaactataca acgcctgctg gacgaacagg agcgcaacca
     1801 gcaacaccag atagaccaga tcatgcgcta cgccactgcg agccgctgcc gccaccagat
     1861 gctggccgcg cacctgggcg aacagataga cgagtgcggc accagttgcg actactgcgc
     1921 cccgcccgcc gaccgccccg acgacgtaca agcccaggca gccgagctac cggacaatcc
     1981 cgggcagatc attattgcgt gcctcatgtc gttccccttc caggcgggca aacccagcct
     2041 ggtgaaggcg ctgaccggct ctgccgccag caacgtgaaa gccgaccgcg tgcgccactt
     2101 cggtgccctg gccgctgcct cacccagcgc gataatgaag gccattgacg agcttgtgga
     2161 ggggggttac ctcgccttct acgagcacga cgaaggcttc aagctcctgc gtgtgactga
     2221 ggagggactt gaaggggtgc ccgccaacgc tgtatctctc aagcagaagc gccagcccaa
     2281 gccgcgcgac cgagcgggcg gggccgtcac tcccaggccg gatcgcgccg cccgcgtgga
     2341 caccgagcgc acgaaggcgg tacgccggtc ggaggaggac agggagccta cccccgaaga
     2401 ggccgacctg ttcgagaggc tgcgccagtg gcggcgggtg atcgcgaacc gcctcggcct
     2461 gcccccatac atcatcttcc acgacaagac gctgtgggcc atcgccaagg cgcagcccac
     2521 cacggtcgag gacctgctgc aaatcaaggg cgtgggccag agccacatcg acaagtacgg
     2581 cgacgagcta ttggaagccc tcgccagctt cgcggaaacg gaagacgaac tggaatcgta
     2641 ggggcgagct ttcccgcgcc gcgatatccc aatccactat cgcgcgggag ggaccatact
     2701 cgctcttgtg acctgggaga gtgtctaaca agcttagggt cactggcaaa agcggcgaag
     2761 caacagacga cgtatagccg gaaagataga gaccggagtg gtctatgagg caaaggtcga
     2821 gacgttcgcc acccgcaccc agttccctgc attcttctca taagtcatta tgcccgcata
     2881 gacgaaccgt acctccctcg cttcctccgc gccctcaagc cgggtagccc gacggacctg
     2941 agtccgaaca atcatccagg ccatgctcgc gtctgcggag atgcgtatga tgggcggctc
     3001 tagatcgtcc cattcgtagt aagtagcgcc cttgaagtag ccctcgaaga aggcgtgctg
     3061 ctcggccagc gtggacctgt gcacttcacc attgctgacg gaaatgaact cgtgtccgct
     3121 gtgcgctagc agcaaatcga catcggtgtc gaaatgcgcc tggcggtcgt gcgcgtgaat
     3181 cttgagaagt gcggccttgt ctaactcaag gtctacagtc gtctcggtat tctccattta
     3241 tccccctgtt cgcgccacta taagagccta ctttctaggt ccaatctgca aag
//
LOCUS       DASRWZ010000122         1789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_969755, whole genome shotgun sequence.
ACCESSION   DASRWZ010000122 DASRWZ010000000
VERSION     DASRWZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969755"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="VFH60_01775"
     CDS             complement(<1..1506)
                     /locus_tag="VFH60_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET6312538.1"
                     /translation="MNQSGKQAHQTSPAKNGKNIPGGHNTVPHRHVHRGRVPGAAHRG
                     REVLSDRKAVRLHTERYRRRMRLVMVLVGLLLTFVVVVGVGVYIVGTTVLNSVSRAAT
                     DIFETPVAQLPRSESGADMPEGTADVVVTFPNWDKKEPVNILLIGLDERTGVEDIRTD
                     TQIIVHIDPDAGTAALVSLPRDLWVEIPGHGEDRINAAYKTGEKAKPGTGATLAKATV
                     RENFGIPIHYYAMVNFKGFEQIVDMLGGVTIDVPRPMVDNEFPFQEFGATRVYIPAGL
                     QHMNGHTALQYARSRHADSDIGRNSRQQQVLLAIKEQGVSLDVMAHLSELAEELGDSV
                     RTDLHWQQIGSLVQLSQSIDGDSIQTVVIAGNMIRETTLPSGANVLMPRWELIRPAIT
                     QAFSDPRLGNEAARLSVKNGTWTAGVARKVRDQLAEIGVYVADLSNAADRGEHPVTTV
                     TDYTGGKKPYTIESVTTMLGIPPGEVKHAPAAQAPIASDGEPVDILVMVGDD"
BASE COUNT          306 a          557 c          545 g          381 t
ORIGIN      
        1 gtcgtctccg accatcacga ggatgtctac cggctcgccg tctgaggcta taggtgcttg
       61 tgcggcgggg gcgtgcttga cctcgcccgg cgggatgccg agcatggtgg tcaccgattc
      121 gatggtgtag ggctttttgc cgccggtgta gtcggtgacg gtggttacag ggtgctcgcc
      181 cctatcggcc gcgttggaca ggtcggccac gtacaccccg atctcggcca gttggtccct
      241 taccttgcgg gccaccccgg ccgtccaggt gccgttcttc accgacaggc gggcggcctc
      301 gttccccagg cgtgggtccg agaacgcctg ggtgatagcg gggcgtatca attcccacct
      361 cggcatgagg acgttggctc cactgggcag cgtggtctcg cgtatcatgt tccccgctat
      421 gaccactgtc tgtatggaat cgccgtctat gctttgcgat agctgcacga gagagccaat
      481 ttgctgccag tgcaaatcgg tgcgcaccga atcgcccagc tcctcggcca gctcggagag
      541 gtgagccatt acgtccaggc tcaccccctg ctcctttatc gccaggagca cctgctgttg
      601 gcgcgaattg cggcctatat cgctatcggc gtggcgggaa cgtgcgtact gaagggcggt
      661 gtgcccattc atgtgctgca accccgccgg tatgtagacg cgcgtcgccc cgaactcctg
      721 gaaggggaac tcgttatcca ccatgggccg gggtacgtca atcgttacgc cccccagcat
      781 atccactatc tgctcgaagc ccttgaaatt gaccatcgcg tagtaatgga tggggatgcc
      841 gaagttctca cgcacggtag ccttggcgag cgtggcgccg gtgccgggct ttgccttctc
      901 gcccgttttg taagcggcgt taatacggtc ctcaccgtgc cccggaattt ccacccacag
      961 gtcgcggggg agcgatacga gggcggcggt cccggcgtcg gggtctatgt gcactatgat
     1021 ttgcgtgtcg gtgcgtatat cttctactcc cgtccgctcg tccagaccta tcagtaggat
     1081 gttgaccggc tccttcttgt cccagttggg gaaggtgacg accacgtccg ccgtgccctc
     1141 cggcatgtca gccccggact cagagcgggg aagctgggct accggcgtct cgaatatgtc
     1201 ggtggcagcc ctcgacacgc tgttcagcac ggtggttccg acgatgtaca ctccaacccc
     1261 tacgaccacc acgaacgtaa gcagcagccc caccagcacc atgacgaggc gcatcctgcg
     1321 ccggtacctt tcggtgtgca gacgcacggc cttgcggtcc gacaggactt cccggccccg
     1381 gtgagcagcc ccaggcaccc gcccgcgatg aacgtggcgg tgaggcacag tattgtgccc
     1441 gccaggtatg ttcttgccgt tcttcgccgg tgaagtctgg tgggcttgtt taccgctctg
     1501 gttcaagaac gaatagctcc tggtccgatc atgctctcgc tacggtcccg gccaaattct
     1561 aatggcgggc ctcaacggtt ggtagcgcaa tatccaccct cgtcataagg ctagcaggtc
     1621 gtagtgcagg gcagttgcat agccgcgtgc gcgtcagcca atgaggccaa catcatgaaa
     1681 gtataaccga aatgtcggcg gtttcaacat caataagggt aaaaccacag acttagtcat
     1741 tctccgccgc cacgacgccc tggtatagcc ctgtgggggt gccctgtgg
//
LOCUS       DASRWZ010000123         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_901680, whole genome shotgun sequence.
ACCESSION   DASRWZ010000123 DASRWZ010000000
VERSION     DASRWZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901680"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..2012)
                     /locus_tag="VFH60_01780"
     CDS             complement(384..2012)
                     /locus_tag="VFH60_01780"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET6312539.1"
                     /translation="MNPAVRYRTTWPLVCAAIALVCLPAMLTASHLAPAYAQPGGRTF
                     PETGKTVSGAFLAYWESNGGLAQQGYPISGEMAEVSDLDGKSYTVQYFERAVFEAHPE
                     NQPPHHVLLSQLGTFQYKKKYPQGAPTQRPNNSPGSRLFAETGKRVGGKFLDYWQKNG
                     GLPQQGYPISEEFTEVSDLDGKSYTVQYFERAVFELHPENRPPYDVLLSQLGTFRYRE
                     KYLAGSAATPTAGRGSGANVTGTWSQAKPMLTHRSEHGAAVLGGKIYVVGGFAGPDRN
                     YTGVSREVEVYDPAADAWSKVAPLPDALHHAAVMASDSLLYVTGGYITTDDPGASNAW
                     VAVKTTWAYDPKADRWSRAADMPQPRAAHAAAFLGGKLYVIGGLDAAVSTELHVYDPA
                     TGTWNTSLPPMPQVREHLAAVGLDGKLYVIGGRYGRNSTLLEAFDPAKNAWERLESMP
                     VGRSGFTAVALDGRIHVFGGESLPTGQVVPGHDTYDPRSNTWVQQTPPIITRHGLPSV
                     VLGGRWYILGGGTSASGGTFQTLSNLVDVFTPNR"
     gene            2316..>3730
                     /locus_tag="VFH60_01785"
     CDS             2316..>3730
                     /locus_tag="VFH60_01785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6312540.1"
                     /translation="MQPLELDTHHTATHVTRRTRQGASLRAQLRARSATIELAALLSV
                     VLLGFVLRAYGLTAHGIWFDEAYHIRLVTLPTVGEMLLAVLSNPPSDPLYVLLLRPWV
                     ALFGHEDFSVRTLSVVLSTATLPATYWLGKVAAGRAAGLVGALLLAVSPYAIEFGQQA
                     SLYVLASLTTTLALAAGLRWYGTGSRRDALLYIALGIAAIYSHYVVAAILGLFGLLSL
                     SRYAGAGPPLIARRAWIAAHVAIFAAWTPWLMGLAASWIATPVPRATLRNPATVEQVL
                     GALVQFSSGTAALLQRARVPEALGLLAGGLLLLSGWLAGREARLRHLRLISVVSGLIF
                     LLPAVASAATGLWLFVPHFMVFLLPAMFVVFGAGLVHLAGWRPTGSDSLTSMLRYLPL
                     ALAAVWLVAQVWGLALFHRYPPHGADGLRELAGILNTATRPDDLVLVTPPALQAGLTQ
                     YYHDETYGIPADFDLFNLYPP"
BASE COUNT          598 a         1189 c         1226 g          717 t
ORIGIN      
        1 gccagggccg ccggggtgta gggcctatcc gcaaactgta ggaagcaagc gaaccaggtt
       61 ggcccttgtc attctgagcg cagcgaagaa tctcatcctc taccttagtg ttgccatttt
      121 ccttagcacc tggcatcact cggcacctga cccacgtcaa gaaaagtggt atttgggtgc
      181 tggaggacga gattcttcgc tgcgctcaga atgacagagg ggagcttggt cccacgcggg
      241 aggcaatttg cagatagccc atagtaatac agagtatgta aagtcaagaa cggtctaact
      301 ggcggggccg tagagggggt aattgccttt ccaaacaagc cttaggggca cggttggcgt
      361 gcccctaagg cttgctagag gggctatcta ttgggcgtga agacgtctac gaggttgctc
      421 aacgtttgga aggtgccacc cgaggcagag gtgccgccgc ccaggatgta ccagcggccg
      481 ccgaggacca cggagggcag gccgtgccgg gtgatgatgg gcggcgtctg ctggacccag
      541 gtgttggagc gggggtcgta cgtgtcatgg cccggcacta cctggcccgt gggcaggctc
      601 tccccgccga atacgtggat gcggccgtcc agggccaccg ccgtgaagcc actcctgccc
      661 acgggcatgc tctccaaccg ctcccaggca ttcttcgccg ggtcaaacgc ctcaagcagc
      721 gtagagttgc gcccgtaacg cccgcctatc acgtacagct tgccgtccag ccccaccgcc
      781 gccaggtgct cgcgtacctg cggcatgggc ggcagggacg tgttccaggt gcccgtggcc
      841 gggtcgtaga cgtgtagctc ggtggatacg gcggcatcca ggccgccaat cacgtagagc
      901 ttgccgccca ggaaggcggc tgcatgggcc gcgcggggct ggggcatgtc ggcggctcgc
      961 gaccagcggt cggccttcgg atcgtacgcc caggtggtct tcaccgccac ccaggcattc
     1021 gaggcccccg ggtcgtcagt ggtgatgtag ccgcctgtga cgtatagcag gctgtcggaa
     1081 gccattacgg cggcgtggtg cagggcatcc ggcagcggcg cgaccttgct ccacgcgtca
     1141 gccgccgggt cgtagacctc tacctcgcgg ctcacgcctg tgtagttcct gtcggggcct
     1201 gcgaagcccc caaccacgta aatcttgcca ccgagcaccg ccgcgccatg ctcggacctg
     1261 tgggtgagca tcggtttagc ctgggaccag gtgccggtta catttgcgcc cgaaccccgc
     1321 ccggcggtgg gtgtggccgc gctcccggcc aggtacttct cccggtagcg gaaggtgcca
     1381 agctgcgaca ggagcacgtc ataaggaggc cggttctcag ggtgcagctc gaacacggca
     1441 cgctcgaagt actgcaccgt gtagctcttg ccgtcgaggt ccgacacctc ggtgaactct
     1501 tcggagatgg ggtagccctg ttggggcagg ccgccgttct tctgccagta atcgaggaac
     1561 ttgcccccta cccgcttgcc ggtctcagca aagagcctgg aaccgggcga attgttgggg
     1621 cgctgcgtgg gagcaccttg ggggtacttc ttcttgtatt ggaaggtgcc aagctgcgac
     1681 aggagcacat ggtgtggtgg ctggttctca gggtgcgcct cgaacacggc acgctcgaag
     1741 tactgcaccg tgtagctctt gccgtcgagg tccgacactt cggccatttc gccggagatg
     1801 ggatagcctt gctgcgcgag gccgccgtta ctctcccagt aggcgaggaa cgccccggag
     1861 acggtcttgc cggtttcggg gaaggtacgg ccgccgggct gcgcgtaagc cggtgccagg
     1921 tgagaggcag ttagcatggc aggcaggcag actaacgcga ttgcggcgca caccagcggc
     1981 caggtcgttc tgtagcgcac ggctggattc atcagatatc ctccccggct gaactccgcg
     2041 tttggactgc tctcgtgacg taagaagcag cccctcagga aagtataatc gacttcggcg
     2101 caggcccggt actcaacaat aagtatgcac accggcgtcg cgatgttccc cggcacaggt
     2161 ggcgaggccg catccccacc agctgctgtc ggtgtatccg ctgcggtctt gaccctgcac
     2221 gcgaactgac ttatcatcgc aatcagtggc ccgtagctaa cggcgcagtg accggatgta
     2281 cgggcagacg gcccaggggt cgcggacgga cagctatgca gccgctggag cttgacacac
     2341 accatacagc cacgcacgtc acccggcgca cgcgccaggg ggcttccttg cgggcgcagc
     2401 tacgggcaag gagcgcgaca attgagcttg ccgccttgtt gagcgtcgtg ctgctcggct
     2461 tcgtgctgcg cgcctacggg ctgaccgccc atggcatctg gttcgatgag gcgtaccaca
     2521 tacggcttgt caccctgccc accgttggcg agatgctgct ggccgtgctc tccaatccac
     2581 cctccgaccc gctttacgtg ctgttgctca ggccgtgggt ggcgctcttc gggcatgagg
     2641 atttcagcgt gcgcacccta tcggtcgtgc tcagcaccgc tacgctaccc gctacatact
     2701 ggctcggcaa ggtagcggcg gggagggcgg ctggcctcgt tggggcgttg ctgctggccg
     2761 tctcaccgta cgctatcgag ttcgggcagc aagcctcgct gtacgtgctt gccagcctca
     2821 cgacgacgct ggcactcgcg gcgggcctgc gttggtacgg caccggcagc cgaagagacg
     2881 ctctgcttta catcgccctc ggcatagccg ccatttacag ccactacgtc gtagcggcca
     2941 tcctggggct gttcggcctg ctgtcgctct ccaggtacgc gggggcaggg ccgcccctta
     3001 tcgcccgccg tgcctggata gcggcccacg tggctatctt cgcggcctgg acgccgtggc
     3061 tcatgggcct ggctgcaagc tggatagcca cgccggtacc acgtgccacc ttgcgcaacc
     3121 cggccacggt agaacaggtg ctgggcgcgc tggtgcagtt tagcagcggc acggcggccc
     3181 tcttgcagcg ggcgcgggtg ccggaggcgc tggggctgct ggcgggcggc ctgctgctcc
     3241 tatcgggctg gcttgcgggc cgggaagcaa gacttcgcca cctgcgcctc atcagtgtgg
     3301 tgagcgggct tatcttcctt ctaccagcgg tggcctcggc cgcgaccggg ctgtggctgt
     3361 tcgtgccgca cttcatggtc ttcctcttgc ctgccatgtt tgtcgtattc ggcgcagggt
     3421 tagtgcacct ggcaggctgg cggccgacgg gtagcgatag cctcacctct atgctccggt
     3481 atctgcccct ggcgctggct gccgtttggc tggtggcaca ggtttggggc ctggcgctct
     3541 tccatcgcta cccccctcac ggtgccgacg gcctgcgcga gcttgccggc atactgaaca
     3601 cggccacgcg gcccgacgac ctggtgctgg tcacgcctcc cgcgctgcaa gccggcctta
     3661 ctcagtatta ccacgatgag acctacggca tacctgcgga cttcgacctt ttcaacttgt
     3721 acccgcccta
//
LOCUS       DASRWZ010000124         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1244403, whole genome shotgun sequence.
ACCESSION   DASRWZ010000124 DASRWZ010000000
VERSION     DASRWZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244403"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1231)
                     /locus_tag="VFH60_01790"
     CDS             complement(104..1231)
                     /locus_tag="VFH60_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6312541.1"
                     /translation="MINLWQVQVFLVVAETGSFSAAAVRLHLTQPGVSQQIRSLETHL
                     GTKLFIRRGHGVELTAAGYDLLDPARRLIHLSEATERTVMSRRSEVSGRIRLGCCVSS
                     APYTMGNWLYEFRSKFPEVSVQLEHTEPGPMIGALRAQELDGGFVLGRMRGRGLVHHK
                     IIEDPITLIVPLNHAWTSPAEIGRAELELQALGREERHGTTAATHAAPSSSSGGGRTP
                     RYGEDGWVPAIKPALLKTQHLIVEHGNGESHSDARRALNDALEERGLTIRDMHVVLEL
                     PDPTAVACAVAEGLGVGLVPLSIARRFVGQVFPLRIEGFSLAQHVYLIHDRKALHSPA
                     VSAWWKFVDGKVAKPSHAESKGESNEREEIAAPVEMAASLV"
     gene            complement(1228..2079)
                     /locus_tag="VFH60_01795"
     CDS             complement(1228..2079)
                     /locus_tag="VFH60_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET6312542.1"
                     /translation="MHSPGPARPPLVDSHAHLDSDDFAGQTEEIVARALDMGLVGVVS
                     IGFEPAGWAPTLQLADRFDLVFPALGIHPNSADQTSEATLSDLADLCRERSVVGLGET
                     GLDYYREYVSHDRQRQSFRAHLDMARQLDLPVIVHNRDAHADVLDILKRDGRGTRGVM
                     HSFSGDLAYARECLALGYMVSLAGPVTFKKALDKHEVAAMVPLDMLLVETDCPYLTPE
                     PFRGRRNEPSYVSYTAEAIARIRGVPLGAVAEATTANAIRLFGLPIRIEPSATWQAEQ
                     TAIEAQP"
BASE COUNT          373 a          685 c          757 g          409 t
ORIGIN      
        1 agaatgacaa aagccacctt tgttcgcagg cttcctacac tttgcagata ctcccaaccc
       61 tcttgagaga tgcctagcta aagggactct gcctggtggc gcattagacc aggctggcag
      121 ccatctccac aggtgcggct atttcttcgc gctcgttgct ttcgcccttg gattcggcat
      181 gactcggttt ggcgactttg ccgtccacga acttccacca ggcgctgaca gcggggctgt
      241 gaagggcctt gcggtcgtgg atgaggtaga cgtgctgggc gagcgaaaag ccctctatgc
      301 gcagcgggaa gacctgcccc acgaagcgcc gcgctatgga gagtggcacc agacccactc
      361 ccaggccctc ggctacggcg caggccacgg cggtcgggtc gggcagctcc agcaccacgt
      421 gcatgtcgcg gatggtgagg cccctttctt cgagggcgtc gttgagggcg cggcgggcgt
      481 cgctgtggct ttcgccgttg ccgtgctcca cgatgaggtg ctgggtcttg aggagggcgg
      541 gctttatcgc gggtacccag ccgtcctccc cgtagcgggg cgtgcgtccc ccgccggagc
      601 ttgagctagg ggcggcgtgg gtcgcggctg ttgtgccgtg tcgctcctcg cggcccaggg
      661 cctgtagctc caactcggcg cggcctatct cggcgggcga ggtccaggcg tggttcaggg
      721 gcacgatgag ggtaataggg tcctcgatga tcttgtggtg caccaggccc cgaccgcgca
      781 tgcgccccag cacgaagccg ccatcaagct cctgcgcccg caacgcccct atcatgggtc
      841 caggctcggt atgttcgagt tgcaccgaca cttcggggaa cttgctgcgg aactcgtaga
      901 gccaattccc catcgtgtaa ggtgccgagg agacgcaaca gcccaggcga atgcgcccgc
      961 ttacctcgct gcggcgcgac atgacggtgc gctcggtggc ctcggacaga tggatgaggc
     1021 ggcgcgcggg gtcgagaagg tcgtacccgg cggcggtcag ttccacgccg tggccgcgcc
     1081 ggatgaagag cttcgtgccg aggtgagtct caagggagcg gatctgctgg ctcacgccgg
     1141 gctgtgtaag gtgcagccgt acggccgccg ccgagaagct gcccgtctcg gccaccacca
     1201 ggaagacctg tacctgccaa aggttgatca tggttgtgcc tcaatagctg tctgttctgc
     1261 ctgccacgtc gcagacggct cgatccgaat cggaaggccg aacaacctga tggcgttcgc
     1321 cgttgtggcc tccgctaccg ccccaagcgg taccccgcgt atgcgcgcta tcgcctccgc
     1381 ggtgtaggag acgtaggagg gttcgttgcg cctgccccgg aacggctccg gggtgaggta
     1441 ggggcagtct gtctccacca ggagcatgtc cagcggcacc attgccgcga cctcgtgctt
     1501 gtcgagggcc ttcttgaacg tcaccggccc cgccagcgac accatgtagc cgagggccag
     1561 gcactctcgc gcgtaggcga ggtcgcccga aaaggagtgc atgacgccac gcgtgccccg
     1621 gccgtcgcgc ttcaggatat cgagcacgtc ggcgtgggcg tcgcggttgt ggacgatgac
     1681 gggtaggtcg agctgccgcg ccatgtccag gtgcgctctg aaggactgtc gctgccggtc
     1741 gtgcgacacg tactcacggt agtagtccaa ccccgtctcg cctaggccaa ccacgctgcg
     1801 ctcccggcac aggtctgcca gatcagagag ggtggcctcg gaggtctggt cggcgctgtt
     1861 ggggtggatg ccgagggcgg ggaagacgag gtcgaagcgg tccgcgagtt ggagggtggg
     1921 tgcccagccc gcaggctcga agccaatcga gacgaccccc acgaggccca tgtcgagggc
     1981 gcgcgccact atctcctcgg tttgccctgc aaagtcgtca gaatcgaggt gcgcgtggct
     2041 gtcaacaaga ggtggtctgg cggggccggg tgagtgcatg tgcaaccctg tcgttacaac
     2101 actattagtg ccgttagctg ccgcagtggg gcggtaggtg cgttgtatgg taccacaccc
     2161 cgcgccgaca ttcaaccgga aagggcttgc attaacagca gattaattgt agaataaatc
     2221 acaa
//
LOCUS       DASRWZ010000125         3805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_285352, whole genome shotgun sequence.
ACCESSION   DASRWZ010000125 DASRWZ010000000
VERSION     DASRWZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285352"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VFH60_01800"
     CDS             <1..994
                     /locus_tag="VFH60_01800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6312543.1"
                     /translation="RDIPVLALSEMRWHPRWLRALPRRMNVALVHVHGQRAHYFVWLM
                     RRLFPRAWGRLPMVATVHGWVQDNFVRKVVTWLEFVTLRDCAHVITVSEQQRRTLLDK
                     GFSPGRVTVVRPGIPYLAAEGLHRATEEERRAARERWGLPFDAYVVTAVGRLSTEKRF
                     DLYLETCAKLAAEIPEARFMLVGGGKQEANLKALSTQLGLEDRLTFTGLTRDMAAVYA
                     ATDLLMLTSDTEGTPHVLLEAMGSDIPVVSTAVGGIPEFVTTEESGLLVPPGSSDALA
                     QAARRVYADPQLASRLVEGGRSVASGFTVDRMTEGVERVYAQVLTARRNKRGTS"
     gene            997..2160
                     /locus_tag="VFH60_01805"
     CDS             997..2160
                     /locus_tag="VFH60_01805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6312544.1"
                     /translation="MPGQRRVRVLQLIDVLGPGGAEMLLHTLASSLDAGRFALHVCGL
                     RPDEPYDLAPQIRALGVPVIGFNQRNAYDIPTLIALVRYIRRHRIDIIHTHLLASDIM
                     GRVAGFLTRTPVVSTVHNSREDFEHEPARRRWMERSTAKLWCRRLIVVSPALREEIAA
                     WFGLPVGRFVAIPNGVDTSRFRPMPDLDKAALKRDLLGDPAARGDTPLVLNVARLVPQ
                     KGLAYLALAARLVLDARPDARFAVVGQGPLRDDLLKQVEELGLDGKFVLAGVRSDVPE
                     LMAAGDVFVLSSLWEGLPISLLEAMSSGCPVVATEVGGVGGVVQSDRTGLLVPAEDPE
                     ALAQAILRCLNNPQAAQQRAAAAREWAHRHYSMETWVRKLEQLYLHEVGRRSR"
     gene            complement(2175..3407)
                     /locus_tag="VFH60_01810"
     CDS             complement(2175..3407)
                     /locus_tag="VFH60_01810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6312545.1"
                     /translation="MITMERPVTIADDFLEELARPVEAASPRAPRVEVVGDVLAFAAL
                     RREWGELLEESGAGIFNSWEWLYPWYRRLGAGRELYILLARDQDGRLVGLMPLCVEHR
                     RAMGRKLRRLAFLGETHVCGDYMDVVSRPEEHGAVVEAFAAHLRQSQGAWDVLDLADF
                     DSQSPTLSMLQRGFSAPEYVAEPRPGSVCPIMSFEPGETFEGFLRGSSRRENYRRRKK
                     QLEKQPGFSVECVEDPAQLAQPFGEFLRLHSLRWSGDGGSDGISGHEVEAFHRDATAL
                     LAERGKLRLFVLRVGGKAVASLYALRHGDTFSYYQAGRDPEWQNMSVGLVMLVETFKR
                     AIEEGATTYDFLRGEEPYKADWNNGQRHTVGLRIYPRGGIGANLGRQERLVASSRGFV
                     KRLLPTSVLQRIRRVRKD"
     gene            complement(3409..>3805)
                     /locus_tag="VFH60_01815"
     CDS             complement(3409..>3805)
                     /locus_tag="VFH60_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6312546.1"
                     /translation="SHNMLAAQDMARVVEVRRRNYRYLLGQLRDVGQPLMPHLHEGVC
                     PLFYPMIVRDKEAIVRKLEAVGIEAIDFWHYFHPSCDPKQFPDAAWLREHVLEVPCHQ
                     DLTPGRMSYVARMVREVVCGETRGGQVGV"
BASE COUNT          643 a         1370 c         1173 g          619 t
ORIGIN      
        1 gcgtgacatt cccgtgctgg ccttgtcgga gatgcgctgg cacccacgct ggctgcgcgc
       61 gctaccgcgt aggatgaacg tcgccctggt gcacgtgcac gggcagcgcg cccactactt
      121 cgtctggctg atgcgcaggc tcttcccccg cgcctggggc cgcctgccca tggtcgccac
      181 cgtgcacggt tgggtgcagg acaacttcgt gcgcaaagta gtaacctggc tggagttcgt
      241 caccctgcgc gactgcgccc acgtgataac cgtctccgaa cagcagcgcc ggaccctgct
      301 cgacaaaggc ttctcgccgg gccgcgtcac cgtcgtccgg cccggcatac cctacctcgc
      361 cgccgagggc ctgcaccgcg ccaccgagga ggaacgccgg gccgccaggg agcgctgggg
      421 cctgccgttc gatgcctacg tcgtcacggc ggtggggcgg ctctctaccg agaagcggtt
      481 cgacctctac cttgagacct gcgctaaact cgcggccgag attcccgaag ctcgcttcat
      541 gctcgtgggc ggcggcaagc aggaggccaa cctgaaggcc ttatcgaccc agctcggcct
      601 tgaggaccgg ctgaccttca ccgggttgac cagggacatg gccgccgtgt acgccgccac
      661 cgacctgctg atgctcacct ccgacaccga gggcaccccc catgtgctgc tggaagcaat
      721 gggcagcgac atcccggttg tgtccacggc tgtggggggc atcccggagt tcgtcacaac
      781 ggaggaaagc ggcttgctcg taccaccggg cagcagcgac gctctcgccc aggccgcgcg
      841 acgcgtctac gccgacccac aacttgcctc ccggcttgtg gagggcggac gtagcgtggc
      901 ttccggcttc actgtggacc gcatgaccga gggcgttgag cgggtgtacg cccaggtgct
      961 aactgcccgc cgaaacaaga gggggactag ctgagtatgc caggacaaag acgcgtgcgg
     1021 gtgcttcaac ttatagacgt gctggggccg ggcggggccg agatgctgct ccacaccctg
     1081 gcttcctcgc tggacgccgg ccgcttcgcc ctgcacgtgt gcggcctccg ccccgacgag
     1141 ccttacgacc tcgcaccgca gatacgcgcc ctcggcgtgc ccgtgatcgg gttcaaccag
     1201 cgcaacgcct acgacatccc cacgctaatc gcgctggtcc gctacatacg caggcaccgc
     1261 attgacataa tacatacgca cctgctcgcc tccgacatca tgggccgcgt ggcgggcttc
     1321 ctcacccgca ccccggtcgt ctccaccgtc cacaactcgc gggaggactt cgaacacgaa
     1381 cccgcccggc ggcgctggat ggagcgctcc accgcgaagc tctggtgccg caggctcatc
     1441 gtggtatccc ccgcgctgcg cgaggagata gccgcctggt tcggcctgcc cgtcggccgc
     1501 ttcgtcgcaa tacccaacgg ggtagacacc tcccgcttcc ggcccatgcc cgacctcgat
     1561 aaggcggccc tgaagcgcga cctgcttggc gaccccgccg cgcgcggaga tacccccctc
     1621 gtcttgaacg tggcgcggct cgtcccgcag aagggcctcg cctacctggc gctggccgcg
     1681 agactcgtcc tcgacgctcg ccccgatgcc cgcttcgccg tggtggggca gggtccgctc
     1741 agagacgacc ttctgaagca agtcgaggag ttgggcctgg acggaaaatt tgtgctggcc
     1801 ggggtcagga gcgacgtgcc cgaactcatg gccgcgggcg acgtgttcgt gctgtcgtcc
     1861 ctgtgggagg gcctgccgat ctcgctgttg gaagcgatgt cttcgggctg cccggttgtc
     1921 gccaccgagg taggcggcgt gggcggggtg gtgcaaagcg accgtaccgg cctcctggtg
     1981 cccgccgaag accccgaagc gctggcccag gcaatactca ggtgcctcaa caatccgcaa
     2041 gcggctcagc agagggcggc ggccgcacgc gaatgggcgc accggcatta cagcatggaa
     2101 acatgggtgc gcaagctgga gcaactttac ctgcacgagg tgggacggcg ttcgcgctga
     2161 accagggact acgctcagtc cttccgcacc cgcctgatcc tctgcaacac cgaggtgggt
     2221 agcagtcgct tcacaaagcc ccgcgacgat gcaaccagac gctcctgccg ccccaggtta
     2281 gccccgattc ccccacgagg ataaatgcgc aggcccaccg tgtgtcgctg gccgttgttc
     2341 cagtcggcct tgtacggctc ctcgccgcgc aggaaatcgt aggtggtggc cccttcctct
     2401 atggcgcgct tgaacgtctc cacgagcatc acgaggccca cgctcatgtt ctgccattcg
     2461 gggtcgcgcc cggcctggta gtagctgaaa gtatcgccgt ggcgcaaagc atacagtgag
     2521 gccaccgcct tgccgcccac ccgcaacacg aacagccgca gcttgccccg ctcggccagc
     2581 agggcagtgg cgtcccggtg aaacgcctcc acctcgtgcc ccgatatgcc gtccgagccg
     2641 ccgtcgcccg accaccgtag cgagtgcagg cgcaggaact ccccgaaggg ctgcgcgagt
     2701 tgcgcggggt cctctacgca ctccacgctg aagccgggct gcttctccaa ttgcttcttg
     2761 cgcctgcggt agttctcgcg ccgcgaggag cctctcagga agccctcaaa cgtctctccc
     2821 ggctcgaagc tcatgatggg gcacacagaa ccaggccgag gctcagcgac gtactcaggg
     2881 gccgagaagc cccgttgcag catcgacagg gtaggcgatt ggctgtcgaa gtccgccagg
     2941 tccagcacgt cccacgcgcc ttgtgactgc cgcaggtgcg cggcgaacgc ctccaccacc
     3001 gcgccatgct cctccgggcg cgacaccacg tccatatagt cgccgcaaac gtgagtttcc
     3061 cccaggaaag ccaggcggcg cagcttgcga cccatggcgc ggcggtgctc cacgcacagc
     3121 ggcatcagcc ccaccagcct gccgtcctgg tcgcgcgcta acagtatgta cagttcgcgc
     3181 cccgccccca gccgcctgta ccagggatat aaccactccc acgagttgaa gataccggcc
     3241 ccgctctcct caagcaactc gccccactcc cggcgcaacg cagcgaaagc cagcacatcc
     3301 ccgaccacct ccacgcgggg ggcgcgcggt gacgcggcct cgacagggcg ggccagttcc
     3361 tccaaaaaat cgtccgctat cgtcaccggg cgttccatcg tgatcatact atacacccac
     3421 ctgcccgcct cttgtctcac cacacaccac ttcccgcacc atccgcgcca cgtaagacat
     3481 ccgccccggt gtgaggtcct ggtggcaggg cacctcaaga acgtgctcgc gcagccaggc
     3541 cgcgtcgggg aattgcttgg ggtcgcacga agggtggaag tagtgccaga agtcgatagc
     3601 ctcgatgccc accgcctcca gcttccgcac tatggcctct ttgtccctca ctatcatggg
     3661 gtagaagagc gggcaaaccc cctcatgcag gtggggcatg aggggctgtc ccacgtcgcg
     3721 caactgcccc agcaggtagc ggtaattgcg ccgccgtacc tcgaccacgc gcgccatgtc
     3781 ctgcgcggcc agcatgttgt ggctc
//
LOCUS       DASRWZ010000126         5777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_833715, whole genome shotgun sequence.
ACCESSION   DASRWZ010000126 DASRWZ010000000
VERSION     DASRWZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833715"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..849
                     /locus_tag="VFH60_01820"
     CDS             124..849
                     /locus_tag="VFH60_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET6312547.1"
                     /translation="MRNVVRSLGVMLVAGALLVPSALAQESGPMAEAEIMGTQGQPVG
                     TAHFTQDGAGAVRVHVIVQGLTGMEGPHGIHVHTVGMCEPDKQFSTAGGHFNPTGAQH
                     GLRNPQGPHVGDLPNINIDASGAGMLEFTSSMLSLTPGPNNIFDEDGAAIVIHAGLDD
                     EVSDPAGNSGASVACGVIRLATMEGPGGTTVGMPRTGASDAGLPWLLALLGAGMAGVG
                     AVARSRGRKKAEIRVRADEADRR"
     gene            876..1850
                     /locus_tag="VFH60_01825"
     CDS             876..1850
                     /locus_tag="VFH60_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HET6312548.1"
                     /translation="MDATNGNTNGVQGIHHITAIAGNAQENLDFYAGVLGMRLVKRSV
                     NQDDPGTYHLFYADAEGHPGTDLTFFPWANMPAGRKGYGLSVEVPLAVPSGSLGYWTE
                     RLTHYGVETAPPETRFGERTLPLRDPHGMELALVETDDPRLFTPWEGSPVPVERQIRG
                     LHAARLWERSLDNTTRFLTSALGFERLGAEGGWVRFGVRGGGSGNLVEIREVPDARLG
                     QWGVGSVHHIAWRVPDDEAELAVRRQVIAAGGRPTEVIDRFWFKSVYFREPGGVLFEL
                     ATDGPGFATDEDPAHLGEQLVLPPWLEASRPGIEAALPELTMPLTTAG"
     gene            2315..2839
                     /locus_tag="VFH60_01830"
     CDS             2315..2839
                     /locus_tag="VFH60_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016860122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6312549.1"
                     /translation="MNVKRTITVESSPEELYNFWHNFENLPRFMQHLESVRVTGDGRS
                     HWKAKAPLGQTVEWDAQVTEDEPNRLIGWHSLEGADVQNSGTVRFTPATGGRGTVVQV
                     EMQYDAPGGPLGVLLAKLFNEEPGQQIFEDLRAFKQVIETGEVVRSDATLSGTRLKQR
                     PGQPPETWPLTESA"
     gene            2901..4073
                     /locus_tag="VFH60_01835"
     CDS             2901..4073
                     /locus_tag="VFH60_01835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010995071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HET6312550.1"
                     /translation="MKANCWMGTKNVEVQEVPDPKILNQHDAIVQISSTAICGSDLHM
                     YNGLLPTMDRGDVLGHEFMGEIVEVGREVKNLKVGDRVIVPFPISCGNCFMCKQGLYS
                     GCENTNPNAWQQELVWGHSTAGVYGYSHMLGGYAGGQAEYARVPFADVNPLKIPAGIE
                     DEKVLFLTDAFPTGYFGADNCDIQPGDTIAIWGAGPIGQFATRSAFLLGAERVIVIDR
                     VDYRLQMARDAGAETVNFEDTDGLLLDVIKDMTGGRGPDACIDAVGMQATGGGLLGVM
                     DHVKQAVKLETDRPLALREVMMAVRTGGRVSIVGDYAGLIDNVPFGAVMNRGITMRMG
                     QAPVQKYYHELLERILNGEIDPSFVATHTLPLGETPKGYELMDNKEDGCVKVVLKP"
     gene            4465..5520
                     /locus_tag="VFH60_01840"
     CDS             4465..5520
                     /locus_tag="VFH60_01840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6312551.1"
                     /translation="MSTTETELVNYVREHSGATINDIASQVPALRAKSYAGVRALVAN
                     LVNRHILKPCGQAPLEQVDGRYERVAACEVCGSPSGEHKTVLWKYNTPVVRCGACGLH
                     YANPRWKTEHLFSRYTADYWEEYAGTISHTSVDEAANNARWFPFLGPHDDVRSTNRLL
                     DVGCATGEFLLAARARGWQVYGVESSPIGAATAARVTGGEVHSGILDTAHYPNGYFDV
                     VTMWDVIEHLQSPRRYLQRIAEILRPGGLLSITTPNIRSLSFWLLGVDWTEIGPNDHL
                     YYFTPRTMARLLDECGFSVYTMHTMAVEPVVWRQWLKYEPLHPFAPLLRNISLPITNR
                     LLLGDELYLIAQRRFDT"
BASE COUNT         1176 a         1758 c         1806 g         1037 t
ORIGIN      
        1 gagaagaggg cggcgcgaat attgcagccg ccctcttttg ctgacttaag ccttcgcatg
       61 gcatgtggct tgcaaaacgt atcggctacg tctaaatatc tacttaatta cggaggagag
      121 attatgagga acgtcgtcag gtcactaggt gtcatgttgg tagcgggggc gctgttggtg
      181 ccctcagcgc tcgcccagga gagcgggccg atggccgaag cagagattat gggcacgcag
      241 ggccagccgg tcggcaccgc ccatttcaca caggacgggg cgggcgctgt gcgcgtgcac
      301 gtcatcgtgc aggggctaac gggcatggaa ggcccgcacg gcatccacgt ccacacggtc
      361 ggcatgtgcg aacctgacaa gcagttcagc accgcgggcg gccacttcaa ccctacgggc
      421 gcacagcacg gcctgaggaa cccccagggg ccgcacgtgg gcgacctgcc caacatcaac
      481 atcgacgcga gcggggcggg catgctcgag ttcacaagca gcatgctctc gctcacgccc
      541 ggaccgaata acatcttcga cgaagacggc gcggccatcg tgatccacgc cggtctcgac
      601 gacgaggtat ccgaccccgc cggtaacagc ggcgcgagcg tggcctgtgg cgtcatccgc
      661 ctggcgacga tggaaggccc aggtggcacg actgtgggta tgccccgcac cggggcgagc
      721 gatgccggtc tgccgtggct gttagccctc ctgggcgcgg gcatggccgg cgtgggcgcg
      781 gttgcaaggt ctcgcggccg caagaaggca gagatacggg tcagagcgga cgaagccgac
      841 cgccgctgag caagtgcgga aaggtagaca aagagatgga cgcaacaaac ggcaacacca
      901 acggggtcca gggcatacat cacatcacgg cgattgcagg gaacgcgcag gagaacctcg
      961 atttttacgc cggtgtgctg ggtatgcgcc tcgtgaagag gagcgtcaac caggacgacc
     1021 ccggcaccta ccacctgttc tacgccgacg ccgagggcca ccccggcacc gacctgacgt
     1081 tcttcccctg ggcgaatatg cccgccgggc gcaaaggtta cggcctgtcg gtggaagtgc
     1141 ctctggccgt gccctccggt agcctgggct actggaccga acggcttact cactacgggg
     1201 tagagaccgc ccctcctgag acccgcttcg gcgagcgtac cctgcccctc cgagacccgc
     1261 acggcatgga actcgcgctg gtggagacgg acgacccgcg cctgttcacc ccctgggagg
     1321 gcagcccggt cccggtcgag cgccagattc gcggcttgca cgcggcccgg ctgtgggagc
     1381 gcagcctcga caacacgacc cgcttcctca cctccgccct tggcttcgag cggctgggtg
     1441 ccgagggcgg ctgggtgcgc ttcggcgtgc gcgggggcgg ctcgggcaat ctggtggaga
     1501 tacgcgaggt gcccgacgcc cggctcggac agtggggcgt gggcagcgtc caccacatcg
     1561 cctggcgcgt gccggacgac gaggcggagc ttgccgtgcg gcggcaggtg atcgcggcgg
     1621 gtggcaggcc caccgaggta atcgaccgct tctggttcaa gtcggtctac ttccgcgagc
     1681 ctggcggggt gctcttcgag cttgccaccg acggccctgg cttcgccaca gacgaggacc
     1741 cggcccacct gggcgagcaa ctagtgctgc cgccctggct cgaagccagc cgcccgggga
     1801 tagaggcggc cctgccggag ctgactatgc cgctcactac ggccggatag gtgcctacag
     1861 aatgtttgtt gtaggctagt agcataccgt gcctggggcc aacgcaacac ttcaagcaac
     1921 aagataaggc ttccactcct gctgtgacag agtggaagcc ttatcttgtt gcttgagtag
     1981 accccaaagt tatcaacgtt tctcacattt tagcgaacgt gatcttgcga aagcgtaatc
     2041 cgtgaaagac cgacgattcg catggcaact atctcagccg gtctggcacc acttctgctt
     2101 acgtatgcaa gcctgccaag caaagtgacg cgcctgcttc gcccctgcta catgttcgcc
     2161 acgagctagc tcagcagccc ccgattggaa caacagccag acaccagaca agctgccacc
     2221 actgtccaac cccacgagtg gccagccaca aagctgctgg aggtgttggg gcatggctgg
     2281 tggagcggcg ctaacctaca aggagaatga cgttatgaac gtaaaaagga caataacagt
     2341 cgagagttcg ccggaggagc tatataactt ctggcacaac tttgagaacc tgccccgctt
     2401 tatgcagcac ctcgaatctg tgcgggtaac gggcgacggc cgctcccact ggaaggccaa
     2461 ggcgccgctc gggcagacgg tcgagtggga cgcccaggtc acggaggacg agccgaacag
     2521 gctgataggc tggcactcgc tggaaggcgc ggacgtgcag aactccggta cggtgcgctt
     2581 caccccggcc accggcgggc gcggcacggt ggtgcaggtg gagatgcaat acgacgcgcc
     2641 gggtgggccg ctgggcgttc tgctcgccaa gctattcaat gaggagccgg gccagcagat
     2701 attcgaggac ctgcgagcct tcaagcaagt gatcgagacg ggcgaggtgg tacgctcaga
     2761 tgctaccctg tccggcacgc gcctgaaaca acgccccggc cagccgccgg agacctggcc
     2821 tctcaccgag tctgcatagt ctgcttcgct cattactacc agcattaccg ttctaccgcg
     2881 aacgaaggag agggacctta atgaaagcca attgttggat gggcaccaag aacgtggaag
     2941 tgcaagaggt gccggacccg aaaatcctga accagcacga cgccatagtc cagattagct
     3001 cgacagccat ctgcggctcc gatcttcaca tgtacaatgg cttgttgccc acaatggaca
     3061 ggggcgacgt gttgggacac gagttcatgg gcgagatagt ggaagtcggc cgtgaggtga
     3121 agaacctgaa ggtaggcgac cgcgtgatcg tgcccttccc gatctcgtgc ggcaactgct
     3181 tcatgtgcaa gcaaggcctc tactcgggct gtgaaaacac caacccgaac gcctggcagc
     3241 aagagctggt gtggggccac tcgacggcgg gcgtgtacgg gtactcacac atgctgggcg
     3301 gctacgccgg tggccaggcc gagtacgccc gcgtgccctt cgcggacgtc aatcccctca
     3361 aaatacccgc gggcatcgag gacgaaaagg tcctcttcct gaccgacgct ttcccaacgg
     3421 ggtattttgg agccgacaac tgtgacatcc agccgggcga cacgattgct atatggggtg
     3481 ctgggccaat tgggcaattt gccactcgca gcgccttcct gcttggcgcg gagcgcgtga
     3541 tagtcatcga ccgggttgac taccgccttc aaatggcgcg tgacgcgggc gcggagaccg
     3601 tcaacttcga ggatacggat ggcttgctgc tggacgtcat caaggatatg acgggtggcc
     3661 gcggcccgga cgcttgcatc gacgccgttg gcatgcaggc cacgggcggc ggtctgctgg
     3721 gcgtcatgga ccacgtcaag caggcggtca agctggagac cgaccggccg ctggccctcc
     3781 gcgaggtgat gatggcagtg cgcaccggag gcagagtgtc aatcgtcggt gattacgcgg
     3841 gcctgatcga caacgtgccc ttcggcgcgg tcatgaaccg tggcataacc atgcgcatgg
     3901 gtcaagcccc ggtgcagaag tactaccacg agctgcttga gaggatcctc aacggggaga
     3961 tagaccccag ctttgtcgcc acgcacacgt tgccgctagg ggaaacccca aaggggtacg
     4021 aactgatgga caacaaggag gacggttgcg tcaaggtggt gctaaagccg taggtccagc
     4081 gcgcgacccg cgcacagaca tgtagtacac ttagcggcta gagccgagcg tacacttaag
     4141 ctcaacaagc atgcagtcct ggtcagcgcc gggatggcat gcttgttggt ttacatgggg
     4201 catgcagttg gcgcatcaag tacagggtgc ggaccaatct tccatgagag gctttccagc
     4261 cgccggacgc aaatctaaat gctgaagtgg tagacatcct ttgtcgtgcc ggcaaaaggc
     4321 agctatactt gtacccctat gcagttaccg aagcttttct cacgcaaaca gagggtcgca
     4381 agtgcggtcc ggtttctatt acaggggtta gaccagtcgc ccctacgatt tcgcgtatca
     4441 cggctctccg gcaatgacac gcatttgtct actactgaga cggaactcgt caactacgtc
     4501 cgcgagcact ccggggccac catcaatgac attgcatcgc aagtacctgc tctccgggcc
     4561 aaatcgtacg ccggggtgcg agcgctcgtc gccaacctgg tcaacaggca cattctcaag
     4621 ccttgcggac aggcaccgct cgagcaggtt gatggccggt acgagcgcgt agctgcttgc
     4681 gaggtctgtg gttcgccatc cggcgagcac aagaccgtgc tgtggaaata caacactccc
     4741 gtcgtgcggt gcggcgcatg cgggctgcac tatgccaatc ccaggtggaa gaccgagcat
     4801 ctgttcagcc ggtacacggc ggactactgg gaggaatatg ccggcaccat aagccacact
     4861 tccgtggatg aagccgccaa caatgctcgc tggttcccct tcctggggcc tcacgacgac
     4921 gtccgttcca cgaatcgcct gctggacgta ggttgtgcta cgggcgagtt tctgcttgcc
     4981 gccagggcgc ggggctggca agtatacggt gttgaaagct cccccatagg cgctgccacg
     5041 gccgcgcggg tcaccggagg cgaggtacac agcgggatac tcgatacggc ccattacccg
     5101 aacggctact tcgacgtcgt caccatgtgg gacgtgatcg aacatctaca aagcccaaga
     5161 cggtacttgc agcgcatagc agagattctt cggcccggcg gcctcctatc gattaccacg
     5221 cctaatatcc gcagcctttc attctggctg ctgggagtcg actggacaga gatcggcccc
     5281 aacgaccacc tgtactactt tactcctcgc acgatggccc gcctgcttga tgagtgtggc
     5341 ttctcggtct atacgatgca tacgatggcg gtagaacccg tggtatggcg gcagtggctc
     5401 aagtacgagc cgctacaccc gttcgctccc ctcctcagga atatatccct ccctatcacc
     5461 aaccggttgc tgctcggcga tgagctgtac ttgatcgcgc aacgccggtt cgacacttag
     5521 gaagaggagg cggccgcagg gttccgtgcg ccatcgagtg cgactggcaa ccacgtatcg
     5581 cgtgcaaaca cgctcaagtg tcgagctagc ggcggcctcc gctgcccgtg cccaaaaata
     5641 aattaacgac caacttgttg agtggatatt gactcggtat tagcagtgtg ctacacttgc
     5701 tcacatctgg atgcttcgta gcatcgctgt ctcttcatac ctccctcttc gtcacccaca
     5761 tccgtgctga gctcgtg
//
LOCUS       DASRWZ010000127         6076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1107733, whole genome shotgun sequence.
ACCESSION   DASRWZ010000127 DASRWZ010000000
VERSION     DASRWZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107733"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VFH60_01845"
     CDS             <1..1320
                     /locus_tag="VFH60_01845"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6312552.1"
                     /translation="VGRVVGSAHAAGDAAIVSATDAHRDGLAQRLKARGVDAALAMSQ
                     GRYVALDAADTLLKFTRDGQPDASLFATLIGGIISGAASAVKHERPRVAAFGEMVALL
                     WADGKPDIAIQVEELWNGLADTHSFELLCAYPMSLFPQSGDGASFGRFCETLSRVIPA
                     ESYTALPGEEERLRAITLLQQKAQALESEIEERKRIEQVLRERNEQLSEAVATRDEFL
                     SVAAHELKTPITSLRGFAQILLRDLGQKREISPERLQFALDTIERQTDKLNQLIARLL
                     DTAQIEAGKLRIEPQRTDLAALVRSVIAQQEGGENHKFVFQGPEQLEAVVDPVRFEQA
                     VTNLLQNAVKFSPEGGVVTVELERAVDGGVQLSVTDQGVGVPPSQRERVFERFHQAHG
                     AGHLSGLGLGLYITREIVALHGGLVWTEEPAHSGSRFVVALPPSSRA"
     gene            1351..1719
                     /locus_tag="VFH60_01850"
     CDS             1351..1719
                     /locus_tag="VFH60_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6312553.1"
                     /translation="MTRVLVVDDEQNIRQVLVYVLEDEGYQVVEAQDGETALGLIERQ
                     HPDVIILDMKMPGMDGWEFVGRYRELYGHQAPIIVLTAARDAAQRGADVSADSYVPKP
                     FDLDVLLERVSSMVRTTPGG"
     gene            complement(2009..2782)
                     /locus_tag="VFH60_01855"
                     /pseudo
     CDS             complement(2009..2782)
                     /locus_tag="VFH60_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720699.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
     gene            complement(2869..3252)
                     /locus_tag="VFH60_01860"
     CDS             complement(2869..3252)
                     /locus_tag="VFH60_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018397370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET6312554.1"
                     /translation="MKLLLDTHVFLWFISGDPRILNVMLQSIRTPENEVYLSVVPLWE
                     VLVKYELGKLPLPQIPESYIPLQRKRHLIDSLPLDEASVSHLANLPPLHRDPFDRMLL
                     CQALEHDLTIVTVDEQIRAYPVRVL"
     gene            complement(3249..3488)
                     /locus_tag="VFH60_01865"
     CDS             complement(3249..3488)
                     /locus_tag="VFH60_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET6312555.1"
                     /translation="MNRISVDEMGRDLPGYIQRVEAGETLVIVRGGKPVAEIKPAAPE
                     TAGLRPFGLCSGEFVVPDDFDMPLPESVMSEFEGR"
     gene            complement(3507..5306)
                     /locus_tag="VFH60_01870"
     CDS             complement(3507..5306)
                     /locus_tag="VFH60_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HET6312556.1"
                     /translation="MDSFDTLEHDVLVIGAGGAGLRATIAAAQAGLNVAVVCKSLLGK
                     AHTVMAEGGIAGALGNLDKADNWQVHFMDTMRGGGFLNNWRTVELLTREAPERVLELE
                     QWGAVFDRTPEGLIMQRPFGGHTYRRLNHVGDRTGLELIRTLQDKAVAAGMKCYMETT
                     IIRLLKDGDRVVGALGYYRETGRFFVIKAKAVILATGGWGRMYKVTSNSWESTGDGAI
                     LAFNAGAELQDMEMVQFHPTGMVWPPGVRGLLVTEGVRGEGGILRNSKQERFMFDSKY
                     MTAAYKGRFSETEDEAARWLEDKANNRPPPELLPRDVVARAIYREVQAGNASEHGGVY
                     LDVTQRGADYIKRKLPSMYEQFHALGDVDITKEPMEVYPTIHYTMGGVRATSETCATN
                     VPGLYTAGETACGVHGANRLGGNALCDLLVFGKRAGEAAAAHAQEHPHGEPDAARIRE
                     EVASMLAPFESAGTENPYVITQELQAAMQEGAMIARTEESLSKCLDKVLELQERAHNL
                     HIEGSRMYNPGWHTARDIQNMLKVSEVIVRCALQRRESRGAQWRLDYPDPDPEWAKKN
                     LIATSGPNGEVQIDTRPVPELPPELAALFEVKK"
     gene            complement(5398..>6076)
                     /locus_tag="VFH60_01875"
     CDS             complement(5398..>6076)
                     /locus_tag="VFH60_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HET6312557.1"
                     /translation="VVLSAFVLYSAFRAFENQFYLVEPYLSPLYSPLIITNWSIFGWT
                     ISPAIYILIFPLSFRLTCYYYRLAYYRAFFWDPPACAVPEPLERRHYSGERQFPFILQ
                     NIHRYALYAALVFIVILAIDAIKGFFFEHGFGVGVGSLVLTVNVILISLYTFSCHSFR
                     HLTGGSVNCYSCTLASRTRHGMWKQISFLNARHSLYAWASLVWVAFTDLYIRSVAAGW
                     ITDFRFF"
BASE COUNT         1076 a         1811 c         1990 g         1199 t
ORIGIN      
        1 gtgggccggg tcgtcgggtc ggctcatgcg gccggggatg ccgcgattgt gagcgccaca
       61 gacgcgcacc gtgatgggct ggcacagagg ctaaaggcgc ggggggtcga tgcggccctt
      121 gccatgtcgc agggccggta cgtggccctg gatgccgctg acaccctgtt gaagttcacg
      181 cgggacggac agccggacgc cagtctcttt gctactttga ttggcggcat tatctcgggc
      241 gcggcttcgg cggtcaagca cgaacggccc agggtcgccg cgtttggcga gatggtcgcg
      301 ctgctgtggg cggacggaaa gcccgacatc gcgatccagg tagaggaact ctggaacggc
      361 ctcgctgata cgcattcgtt tgagttgcta tgcgcctacc cgatgagcct cttcccgcag
      421 tcgggggatg gtgcgtcttt tggcaggttc tgtgaaacgc tctctcgcgt catacccgcc
      481 gagagctaca cggcgctgcc gggtgaagag gagcggctgc gtgctatcac cctcttgcag
      541 cagaaggcgc aagccctcga aagcgagata gaggagcgca agcgaatcga gcaagtgctc
      601 cgcgagcgga acgagcaact gagcgaggct gtggcgaccc gcgacgagtt cctgtcggtg
      661 gccgcgcacg agcttaagac ccccattacc agcctacggg gcttcgcgca aatactgcta
      721 agggacctcg gacagaagcg ggagatttcg cctgagcggt tgcagttcgc cctcgacacc
      781 atcgaacggc agacggacaa gctcaaccag ttgatcgcgc gcctcctcga tacggcgcaa
      841 atcgaggccg ggaagctgcg aattgaaccg cagcgcaccg accttgccgc gctcgtccgc
      901 tcggtaatcg cgcagcaaga gggcggcgaa aatcataagt tcgtctttca aggccccgag
      961 cagctggaag cggtggttga cccggtacgc ttcgagcagg cggtcaccaa tttgctgcaa
     1021 aacgcagtca aattcagccc cgaaggcggg gtagtgaccg ttgagctgga gcgagccgtg
     1081 gatggtggcg tgcagctttc ggtgacggac cagggtgtgg gcgtgccgcc gagtcagcgg
     1141 gagcgggtgt ttgagcgttt ccaccaggcg catggggcgg gccacctgtc cggcctgggc
     1201 ctcggccttt acatcacccg cgagatagtg gcgttgcacg gcgggcttgt ctggaccgag
     1261 gagcctgcac attccgggtc gcgcttcgtt gtagcccttc cgccctccag ccgagcctag
     1321 gcgtggcccg tcagcccaac cggtgagcgc atgactcgtg tccttgtggt tgacgatgag
     1381 cagaatatac gccaggtgct tgtctacgtg ctcgaagatg agggctacca ggtggtggaa
     1441 gcgcaagacg gcgagaccgc cctggggctg atcgagcggc aacaccccga cgtgattatc
     1501 ctcgacatga agatgccggg catggacggc tgggaattcg tcggccgcta ccgcgagcta
     1561 tacggccacc aggctccgat catcgtcctc acagccgcgc gggatgccgc ccaacgaggg
     1621 gccgacgtga gcgccgacag ctacgtgccc aagccgttcg atctggacgt gctgctcgaa
     1681 cgagtgtcgt ctatggtcag aacgacccct ggcggttgaa accgcgggca acagctacga
     1741 agtcccccta cggggactta ttttcattgt ctgtagcagg gctgacctca ccgcctatta
     1801 cttccgcgta gcctgatctg ggcacggccc tggccctgta ggggttctgc gggtcgaaga
     1861 tgatgccgtc cttgctcaca ccgggagggc catatggcct cggcggggct tcaggttccg
     1921 gcgggggagc cgtgctgctg attgcgggtg cgttgtatcc cacgctggac gtgcgtgtgt
     1981 ccttgctcct ggttaccttg cgggcggccc acatcaccgg gtcgaagtaa atgtcggcca
     2041 cccgctcctt gagggggatg ataccgttgt ctgtaaggtt gatatgctca gggcagacct
     2101 ccgcgcagca cttggtgatg ttgcagaagc cgacgcccgc ctcctcgcgc gccatctggc
     2161 ggcgttccag gccgtcgtag gggtgcatat cgaggttgga gagcttgatg agctggcgcg
     2221 ggccgaagaa ggcgtccttg cggttgtggt cgcgcaggat gtggcaggtg tcctggcaga
     2281 ggaatcactc gatgcacttg cgaaactcct ggatgcggtc cacgtctatc tgctgcatga
     2341 ccaaaggtgt gggtgcgtcg gccctgggtg tgaagggtgc gacgcgttcc gacatgcggt
     2401 agttccacga cacgtcggta acgaggtctt tcaccagcgg gaacgccttg agcgggtgga
     2461 ccaggatggg cgcgtcgccg tctgcgtagt cgcgcacgcg ggccttgcag agcagtcgag
     2521 gcttgccgtt gatttcggcg ctacaagagc cgcacttgcc cgccttgcag ttccaccggc
     2581 aggagagggt gctgtcgaac tcggcctgga tgtagtggat ggcgtcgagc actaccatgc
     2641 cggggaaggt gggcacctcg tagtcggact cggtgccctc ctcggcggtg ccccggaaga
     2701 cgcgcagggt gacagtctgc ccgacggagg aagtggggcg tttggcctcg cccttgtcct
     2761 cgaaggtcgc ctcccggtcc atcggcatac tgcccagctt ggcctctgtt tcggtggtca
     2821 tggtgttctc ctagacttta ttacttccat tctgaaaatt gctccgtatc aaagaaccct
     2881 cactggataa gctctaatct gctcatcaac cgtcacgatt gtcaggtcat gttctaaagc
     2941 ctgacataac agcatcctgt caaatgggtc tcggtggaga ggcggcagat tggcaaggtg
     3001 gctcacacta gcctcatcca gcggcaaact gtcaatcaga tgcctcttac gctgtagtgg
     3061 tatgtagctc tccggtattt gaggtaaggg tagtttgccg agttcgtatt tgacgagcac
     3121 ttcccataga ggcaccacgc tgagatagac ttcgttttcc ggcgttcgaa tactttgtaa
     3181 catgacattg agtatacgtg gatctccact gatgaaccac aggaagacgt gtgtatccag
     3241 caggagtttc atcttccctc gaattcgctc atgacgcttt ccggtaaggg catgtcgaaa
     3301 tcatcgggca cgacgaactc gcctgagcat agcccgaacg gccgcaaacc tgccgtttct
     3361 ggggccgctg gtttgatttc ggctacgggc ttgccgcccc gcacgatgac cagagtctct
     3421 ccagcctcta cacgctgtat gtagcctggc aaatcgcgtc ccatttcatc tacactaatc
     3481 cggttcatat gtttgcctct gactgcttac ttcttcacct cgaacagcgc cgcgagttcc
     3541 ggcggcagct ccggcaccgg gcgagtgtcg atttggacct cgccgttagg gccgctggtg
     3601 gcaatgaggt tcttcttggc ccattcgggg tcggggtcgg ggtagtcgag ccgccactgc
     3661 gcgccccggc tctcgcggcg ttgcagggcg cagcgcacga tgacctcgga caccttgagc
     3721 atgttctgga tgtcgcgcgc cgtgtgccag ccggggttgt acatccgcga gccttcgatg
     3781 tgcaggttgt gagcgcgctc ctgcaactcc agcaccttgt ccaggcactt gctcaggctc
     3841 tcttcggtgc gggctatcat cgcgccttcc tgcatcgccg cctgcaactc ctgggtgatg
     3901 acgtaggggt tctcggtgcc cgccgactcg aagggggcta gcatggaggc tacttcctcc
     3961 cgtatgcggg ccgcgtcagg ttcgccgtgc gggtgttctt gcgcgtgcgc ggctgccgcc
     4021 tcgcccgccc gcttgccgaa caccagcagg tcgcacaggg cgttgccccc caggcggtta
     4081 gcgccatgca ccccgcacgc cgtctcgccc gcggtgtaga ggccgggcac gttggtggcg
     4141 caggtctcgg acgtggcccg cacgccgccc atcgtgtagt ggatggtggg gtagacctcc
     4201 atcggctctt tcgtgatgtc cacgtcgccc aacgcgtgga actgctcgta catgctcggc
     4261 agcttgcgct tgatgtagtc cgcgcccctc tgcgtcacgt cgaggtagac cccgccatgc
     4321 tcgctggcgt tacccgcctg tacctcccgg tagattgcgc gggccaccac gtcgcggggt
     4381 agcagctcgg gcggcggccg gttgttggcc ttgtcctcca gccagcgggc ggcctcgtcc
     4441 tcggtctccg aaaatcgccc cttgtaagcg gcggtcatgt acttcgagtc gaacatgaag
     4501 cgctcttgct tggagttgcg caggatgccg ccttcgcctc gcacgccttc cgtgaccagg
     4561 aggccccgca ccccgggagg ccacaccatg ccggtggggt ggaactgaac catctccatg
     4621 tcctgcaact cagcgccagc gttgaaggcg agtatggccc cgtctcccgt ggactcccag
     4681 gagttggaag tcaccttgta catgcggccc cagccacccg tagccagtat cacggccttg
     4741 gccttgatga cgaagaagcg gccggtttcg cggtagtagc cgagcgcgcc taccacgcgg
     4801 tctccgtcct tcaggagcct gatgatggtg gtctccatgt agcacttcat accggcggcg
     4861 acggccttat cctggagggt gcggatcagt tcgaggccgg tgcggtcgcc cacgtggttg
     4921 aggcggcggt aggtgtggcc gccgaagggg cgctgcatga tgaggccctc cggcgtgcgg
     4981 tcgaacacgg ccccccactg ctccaattcg agcacgcgct ccggggcctc cctggtgagc
     5041 agctccacgg tgcgccagtt gttgaggaag ccgccgcccc tcatggtgtc catgaagtgc
     5101 acctgccagt tgtcggcctt gtcgaggttg ccgagggcac cggcgatgcc gccctccgcc
     5161 atcaccgtgt gggccttgcc gagcagcgac ttgcagacga cggccacgtt cagcccggct
     5221 tgtgccgccg ctatcgtggc ccgcagcccc gcgccccccg cgcctattac gagcacatcg
     5281 tgttcgaggg tatcgaaaga gtccatagtc ggtagtcaat ccaatcagtt agtatgagca
     5341 gtagatgcga gaaagtcaga gggagcggcg tattgcttgc ctggtctcgc ctcgtatcta
     5401 gaagaaccgg aagtcagtaa tccaacccgc cgccactgag cgtatgtaca ggtcggtgaa
     5461 ggctacccag acgaggctgg cccaggcgta cagggaatgg cgggcgttga ggaagcttat
     5521 ctgcttccac atgccatgcc gcgtcctgct ggcgagggtg caagaatagc aattgacgct
     5581 gcccccggtg aggtgccgga atgagtggca cgagaaggtg tacagggaga tgagtatgac
     5641 gttgaccgtg agcacgagcg acccgacgcc cacgccgaaa ccgtgctcga agaaaaagcc
     5701 cttgatggcg tcaatcgcca ggatgacgat gaacaccagg gccgcgtaga gggcgtagcg
     5761 gtggatgttc tgcaggatga aggggaactg gcgctcgccg ctgtagtgcc tgcgctccag
     5821 cggttccggt acggcgcacg cgggcgggtc ccagaaaaag gcccggtagt aggcgaggcg
     5881 ataatagtag caggtcagcc ggaagctgag aggaaagatg aggatgtaga tagccgggga
     5941 gatcgtccag ccaaagatgc tccaattggt gatgattagg ggcgagtaga gcggggaaag
     6001 gtacggctcc acgaggtaga actggttctc gaacgcacgg aacgccgagt agaggacgaa
     6061 cgcgctcaag accacc
//
LOCUS       DASRWZ010000128         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_628099, whole genome shotgun sequence.
ACCESSION   DASRWZ010000128 DASRWZ010000000
VERSION     DASRWZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628099"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFH60_01880"
     CDS             <1..702
                     /locus_tag="VFH60_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET6312558.1"
                     /translation="DTKVEVRWVNTETLEQQGPNDLLDGVDGIVVPGGFGDRGVMGKV
                     IAAQYARENNIPYLGLCYGLQMAVIEFARNVLGLPGAFTTEIDSRCPDPVIDIMPDQR
                     DIADMGGTMRLGSYPCNLVPGTKAALAYGETVVHERHRHRFEFNNAYRERMREAGLLV
                     SGLSPDGRLVEIIELRDHPWFVATQAHPELKSRPTRPHPLFRDFVKAVKAYSQESQDG
                     EEGEDKPLDMRRIVA"
     gene            1067..>2820
                     /locus_tag="VFH60_01885"
     CDS             1067..>2820
                     /locus_tag="VFH60_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312559.1"
                     /translation="MAVAVAEPKVEAPAVTRTRRRISLDWGMAALMFVLAFAFMAPGL
                     PPLRVAAPMEFLLPFHPWQRYYPEITSPFLGGDLLLQQLPWHHWAQDEWAAGRFPLWA
                     SGPAGGMPLHASSQAAVLYPLHLLWALLPVGAGLGIIMALKLWLAGLGMWGFLRVLGL
                     HPAAVGLSALSFMFSTSLVNWLPWQLSGLILLLPWLLWAVYLWWTKESRPALVGVAAL
                     VGFMVLGGHAETLCILGLTTAGWTLGVMLVSGGTWRSRLRRLAGTVLAAGLGFLLGMV
                     QLLPFLDAVGLTHQFAARQAQDVATASTFKLRPEFLLNWLVPRSQGYLPDLVLPRSFG
                     FTEGNGYVGLVPVVGLGLAIVSAFRRRLKLSLALPWLCIGAFAFLVTYDGTVGPFIRA
                     LPGFAQSINVRWVMVVGFSIIMLGAFGWDWLARAVLAPKADGESTWHRLALVEAVTLV
                     ALGGVVMAAHVLSLLPYPVMEHIGPTWLQLNNTYGWYWAVWAAGLFVAVLGLAFLWLV
                     WRRGPRVAPVLLGLLLLADLWWLLIPINGTSPAEQYYPVTEFIRQVKAAVPPTERILI
                     EDDVMPANTGLVYNIRDWR"
BASE COUNT          430 a          924 c          914 g          552 t
ORIGIN      
        1 gacaccaagg tggaagtgcg ctgggtcaac accgagacgc tcgagcagca aggccccaac
       61 gacctgctgg acggcgtgga cggcatagtg gtgcccggtg gcttcgggga ccgtggcgtg
      121 atgggcaaag taatcgccgc ccagtatgcg agggagaaca acatacccta cctggggctg
      181 tgctacggct tgcagatggc ggtcatcgag ttcgcccgca acgtgctcgg cctgccgggg
      241 gccttcacca ccgagatcga ctcgcgctgc ccggaccccg tgatcgacat catgcccgac
      301 cagcgcgaca tagccgacat gggcggcacg atgcgcctgg gttcctaccc ttgcaacctc
      361 gtgcccggca ccaaagccgc gttggcctac ggcgagaccg tagtgcacga gcggcaccgg
      421 caccgcttcg agttcaacaa cgcctaccgg gagcgcatgc gggaggcggg cctgctcgtg
      481 tcgggcctgt cgccggacgg caggctggtg gagatcatcg aactgcgcga ccacccctgg
      541 ttcgtggcta cccaggccca ccccgagctg aagagccgcc ccacccgccc ccaccctctc
      601 ttccgcgact tcgtgaaggc cgtcaaagcc tatagccagg agtcgcagga cggcgaggaa
      661 ggcgaggaca agccgctcga tatgcggaga atagtggcat aactgcttcg ggacgcagcc
      721 ggttctaagc ggagaaaggg gcacctgggt aggctgcccc tttgtcttac agtttagggt
      781 acaatggagc gttcctggca cggtctgtcg taaagtagaa ttgcaggacc aactttccct
      841 ttgtcattct gagcgtagcg aagaatctcg tccctcaccg gaacgttccc cttttcttct
      901 gagggtgtgg tactagggga aaggtgttgc ctgggtgctg gaggacgaga ttcttcacta
      961 cgttcagaat gacaaaggcc aatatagtac ccgttgccaa cggattcgta ggcgatagtc
     1021 aatggcgcgg gcactagtcc tataacccgg aagaagctag caacccttgg cagtagcagt
     1081 cgctgaaccc aaagtagaag cccccgccgt gacgcgtacc cggcggcgca tctcgcttga
     1141 ctggggcatg gccgcgctca tgttcgtgct ggcttttgcc tttatggcac ctggcttgcc
     1201 cccgctcagg gtagccgcgc cgatggagtt cctgctgccc ttccacccct ggcagcgcta
     1261 ctacccggag ataacgtcgc ccttcctcgg cggcgacctg ctcttacagc agctcccctg
     1321 gcaccactgg gcgcaggacg agtgggccgc gggccgcttt cccctctggg cctccggtcc
     1381 cgccgggggc atgccgttgc acgcaagctc gcaggcggcc gtgctttacc cattgcacct
     1441 gctgtgggcg ctcctgcccg tgggcgcagg cctcggcatc atcatggcgc tcaagctctg
     1501 gctggcgggc ctgggcatgt ggggcttctt gcgggtgctc ggcctccacc cggcagcggt
     1561 ggggctatcg gccctcagct tcatgttctc cacctccctt gtgaactggc tcccctggca
     1621 gctttcgggc ctcatactac tgctgccctg gctgctgtgg gccgtctact tgtggtggac
     1681 caaagagagc cgccccgcgc tcgtgggggt ggccgcgctc gtgggcttca tggtgcttgg
     1741 cgggcacgcc gagacgctgt gtatcctggg cctgaccacc gccgggtgga cgctgggcgt
     1801 catgctggtt tccggcggca cgtggcggtc gcgcctgcga cggctggcgg gtacggtgct
     1861 cgccgcgggg ctgggtttcc tgctaggcat ggtccagttg ctgcccttcc tggacgccgt
     1921 aggtttgacg caccagttcg cggcgcggca ggcccaggac gttgccaccg cctcaacctt
     1981 caagctgcga cccgagttcc tgctcaactg gctggtgccc cgcagccagg gctacctacc
     2041 cgacctcgtg ctaccgcgct ccttcggctt cacggagggc aacggctacg tggggcttgt
     2101 gcccgttgtg ggtctgggcc tcgccatcgt gtctgccttc cggcggcgtc tgaagctgtc
     2161 gctggccctg ccgtggctct gcataggcgc gttcgccttc ctcgtcacct acgatggcac
     2221 agtaggccca ttcatccgtg cgctccccgg tttcgcgcag agcataaacg tccgctgggt
     2281 catggtggtg ggcttctcta tcatcatgct gggcgcgttt ggctgggact ggctggcgag
     2341 ggcggtcctc gccccaaaag ctgacggcga gagcacatgg catcgcctgg cgctggtgga
     2401 ggccgtcacc ctggtagcgc tgggcggggt tgtcatggcg gcccacgtgc tgagcctgct
     2461 gccctacccg gttatggagc acattggccc aacgtggttg cagctcaaca acacctatgg
     2521 ctggtactgg gccgtgtggg cggcggggtt gttcgtagca gtgctcggcc tggcgttcct
     2581 gtggctcgtc tggcggcgcg gcccgcgggt ggccccggtg ctgcttggcc tgctgctcct
     2641 ggccgacctc tggtggctgc tgatccccat caacggcacc tccccagccg agcagtacta
     2701 ccccgtcacc gagttcatcc gccaggtgaa agccgccgtg ccccccaccg agcgcattct
     2761 catcgaagat gacgtaatgc ccgccaacac cgggctggta tacaacatcc gcgattggcg
//
LOCUS       DASRWZ010000129         1555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_559873, whole genome shotgun sequence.
ACCESSION   DASRWZ010000129 DASRWZ010000000
VERSION     DASRWZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559873"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1507
                     /locus_tag="VFH60_01890"
     CDS             245..1507
                     /locus_tag="VFH60_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019734954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HET6312560.1"
                     /translation="MEQWLALAVFVVTFAAIIVEKVHKTLVALAGAMAMLLLGLISQE
                     RAFEGVDWNVIFLLAGMMIIANALRRTGIFRWTAMKMIDIAKGEPVAVLLLLSTLTAV
                     ASAFLDNVTTVVLMVPLTLFVANALAISPVPFLISEILASNIGGAATLIGDPPNIIIG
                     SASGLDFGALLFNMGPLILVVYLVFAGLTVLFFRKELKTSLRHRAEAIAMPEEGVIRD
                     RRLLVQSMIVLVAVILGFLLHGFLHYEAATVALTGAVALMLFANLEVHEVLSELEWTT
                     LLFFIGLFVVVEALVEVGWIEMAARAMLDLTGGNEAVTAMILLWGSGVASGIVDNIPY
                     TATMVPIVQELSATMRPEPLWWSLAIGACLGGNATAVGASANVIVLSLADRAGYKISF
                     MTFLKYGLIVTFVSLAISTVYVWLRYLM"
BASE COUNT          261 a          432 c          519 g          343 t
ORIGIN      
        1 ataaacgggg actgtggatt aagtatattc aacttcaata tgcgtagctc ggacatgacc
       61 ggctcttctg cttgcctgcg tcctaccgtc tcgcttataa tcgaccggat agttgtgcag
      121 cgtcgaaatc gcaagtgcaa ctggggccct gagcgtgcag accagcgctc agggttttgg
      181 cttgttttag gggtgagttg ctgctgatgc ttgtctcgcg cgactataag aaaaggggta
      241 gctaatggag caatggctgg cgcttgcggt gttcgtggtt accttcgcgg cgatcatagt
      301 ggaaaaggtg cacaagacgc tggttgccct ggctggcgcg atggcgatgt tgctgttggg
      361 gctgatctca caggagcggg cgttcgaggg cgttgactgg aacgtgatct tcctgctcgc
      421 aggcatgatg attatcgcca acgccctgcg acgcaccggc atcttccggt ggaccgcgat
      481 gaagatgatt gacatagcta agggcgagcc tgtggccgtg ctgctgctcc tctccaccct
      541 caccgccgtc gcctcggcct tcctcgacaa cgtgaccacg gtggtgctca tggtgcccct
      601 caccctgttc gtggccaacg ccctggcgat aagcccggtg ccgttcctca tctccgaaat
      661 cctggcctcc aacatcggcg gggccgccac gctgataggc gaccctccca acatcatcat
      721 cggcagcgcg tcgggcctcg acttcggggc gctcctgttc aacatggggc cgctcatact
      781 ggtggtctac ctggtgttcg ccgggctgac ggtgctcttt ttccgcaagg agctaaagac
      841 cagcttgaga caccgcgccg aagcgatagc catgcccgaa gaaggagtca tacgcgacag
      901 gcggctgctc gtccagtcca tgatcgtact ggtggcggtt atcctgggct tcctgctgca
      961 cgggttcctg cactacgagg cggcgaccgt ggccctcacc ggcgcggtgg cgctgatgtt
     1021 gttcgccaac ctagaggtgc acgaggtgtt gagcgagctt gagtggacga cgctgctgtt
     1081 cttcattggg ctgttcgtgg tggtcgaggc gctggtggag gtggggtgga tagagatggc
     1141 ggccagggcc atgctcgacc tgacgggggg caacgaggcg gttacggcca tgatcctgct
     1201 ctggggcagc ggggtagcct ccggcatcgt ggacaatatc ccttacacgg ccacgatggt
     1261 gcccatagtg caggagctga gcgccacgat gaggccggag ccgctctggt ggtccctggc
     1321 gattggggcg tgcctgggtg ggaacgccac ggcggtgggg gcctcggcca acgtgatcgt
     1381 gctcagcctg gccgaccgcg ccgggtacaa gatcagcttc atgacgtttc tgaagtacgg
     1441 cctgatagtc acgttcgttt cccttgcgat cagtacggtg tacgtgtggc tgcggtacct
     1501 gatgtagggg agtggtcagt ggtcagtggt cagttgcatc agtgccctgc gggga
//
LOCUS       DASRWZ010000130         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_80105,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000130 DASRWZ010000000
VERSION     DASRWZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80105"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..979
                     /locus_tag="VFH60_01895"
     CDS             350..979
                     /locus_tag="VFH60_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312561.1"
                     /translation="MILHSRKWRLVIRVMTLALTSMLMLAFAGAASYALQDDSPQPLT
                     TPAQLHDKRKPLADTYVNVTAAPNLARAQSTQELLGERYWIPLDGYGYQLFVRTDSGQ
                     YLFPYDYAPEMGFIGAGAPVLYSGKITPLKGQAGADDLVQQLAEQGVTVDKGKAMVLL
                     LNEAPSAYRPVVPVMPVLAWMWLGAFVGLVQIWRGKGRVIRGAPRPRTA"
     gene            1134..2534
                     /locus_tag="VFH60_01900"
     CDS             1134..2534
                     /locus_tag="VFH60_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6312562.1"
                     /translation="MFFKQFVNEDLGCASYIIGDTDAGECVVVDPGWDVTPYVGLASN
                     KGMAIRYVIETHNHADHVSGHGKLARLGAQVAVHEEAGVTYPHKPLADGEVLAIGEVE
                     LRVIHTPGHRPEHVALAVTDTSRAGEPWLVLTGDSLFVGDVGRPDLAVEPDEGAAQLF
                     TSLNTLLKLEDGVEVYPGHVAGSLCGKSMSPKPNSTIGFERRYNAPLAETERGAFVRR
                     VTAELPPQPPQFGRIVEKNRGPFLTEDAVVRALTADEVEALRKEGAFVLDTREPDAFG
                     GGHIPGAINVDLHGGQFATRASWVIPEGVPVVLVLGAPGDLKQAMSLLEAVGQGNVRG
                     HLLGGMHAWDSSGRPIETVPQVPVAELRERLQSGDRSFTVLDVREDSEWRDGHIEGAI
                     HIPFHQLTKSLDRVPSHKPVAVVCGGGTRSSIAASLLQGEGFDPMNVSGGMDAWNSSG
                     YPLAQASFSAGADAAR"
BASE COUNT          503 a          847 c          873 g          477 t
ORIGIN      
        1 agggcaggca cgggggcctg cccctacgca gcacccattc gtgcctggcc attacctgat
       61 taaccagtta atcttcatta gtggcagggc actcccacag ggctgttgct cgttaccatc
      121 atgcccggcc ggaccagtag cctggactct ctttgcgcgt cctctgcgtt ctctgcgtcc
      181 tctgcgttct ctgcgttctc tgcggtgaat aattcagtct cacaagtcac ccaggtgctt
      241 tcctatgggt gagctttgca tggtataatt atggggcgtg aaacttgctt ctgaacatca
      301 gcctaagaca cgtacagaca cagcgcgggg cgacaggaga gcgtaggaga tgatacttca
      361 cagccgcaag tggcggctgg taatacgcgt gatgacgctg gcattgacca gcatgcttat
      421 gcttgcgttc gcgggcgcgg cttcgtatgc cttgcaggac gacagccccc agcctctcac
      481 cactcccgcc cagcttcacg acaagcggaa gcccctggcc gatacctacg tgaacgtcac
      541 cgccgcgccc aacctggccc gcgcccagtc cacgcaggag ttgctcggcg agcgctactg
      601 gatacccctc gacggctacg gctaccagct attcgtgcgc accgacagcg gccagtacct
      661 cttcccttac gattacgccc ctgagatggg cttcataggc gccggcgctc ctgtgctcta
      721 cagcggcaaa attaccccgc tcaagggcca ggcaggggcc gacgacctgg tgcagcaact
      781 ggcagagcag ggcgtgaccg tggacaaagg caaggcaatg gtgctccttc tcaacgaggc
      841 ccccagcgcc taccgccccg tggtgcccgt catgccggtg ctcgcctgga tgtggctagg
      901 cgctttcgtc ggcctggtgc aaatctggcg aggcaaaggc cgcgtcattc gtggcgctcc
      961 caggccccga acggcgtagg aagtaatgag tgatgaggac tgaggactga ggactgagga
     1021 ctgagtgtac cccatcccaa tatccttaaa ctttgcgcgt cctttgcgtc tttgcgtttt
     1081 cgtctcttaa ctcagtcctc agccctcatc actcattact cgtggggtga atcatgttct
     1141 tcaagcagtt tgtcaatgaa gacctggggt gcgcgtccta tatcataggg gatacggacg
     1201 cgggtgagtg cgtcgtggtc gatccggggt gggatgtgac accttacgtg gggcttgcct
     1261 ccaacaaggg catggctatc cgctacgtga ttgagacgca caaccacgcc gaccacgttt
     1321 cggggcacgg caagctggcg cggctgggcg cgcaggtggc cgtccacgaa gaggcgggcg
     1381 tgacttatcc ccacaagccg ctcgctgatg gcgaagtgct cgcgataggc gaggtcgagc
     1441 ttcgggtgat acatacgccc ggacaccgcc cggagcacgt cgccctcgcc gtgaccgata
     1501 cctcccgcgc cggggaaccc tggctggtgc tcaccggcga ctccctgttc gtgggcgacg
     1561 tgggcaggcc cgacctggcg gtggagccgg acgagggggc ggcccagcta ttcacgtcgc
     1621 tgaacaccct gctcaagctg gaggacggcg tggaggtcta tccggggcac gtcgctggct
     1681 cgttgtgcgg caagtcgatg agtcccaagc ccaactccac tataggcttc gagcgcaggt
     1741 acaacgcgcc cctggccgag acggagcgcg gggccttcgt gcgtcgggtc accgccgagc
     1801 tgcccccaca gcctccccag tttggccgca tcgtggagaa gaaccgaggc cctttcctca
     1861 ccgaagatgc cgtagtgcgc gccctcaccg ccgacgaggt agaagctctg cgcaaggaag
     1921 gcgctttcgt gctggacacc cgcgagccgg acgctttcgg gggcgggcac atacccggtg
     1981 ccatcaacgt ggacctgcac ggcgggcagt tcgccacccg cgcctcgtgg gtcataccgg
     2041 agggggtgcc ggtggtgctg gtgctgggcg cgccgggcga cctgaagcag gcgatgtcgc
     2101 tgctcgaggc ggtggggcag ggcaacgtca gggggcattt gcttgggggt atgcacgcct
     2161 gggacagcag cggcaggccg atagagacgg tgccgcaggt gcccgtggcg gaactgcgcg
     2221 agcggctgca atcgggcgac cgcagcttca ccgtgctcga cgtgcgcgag gactcggagt
     2281 ggcgagacgg ccacatcgag ggggcgatac acatcccgtt ccaccagcta acaaagagcc
     2341 tcgaccgggt gcccagccac aagccggtgg cggtggtgtg cggcgggggc acgcgtagca
     2401 gcatcgcggc cagcctccta cagggcgagg gcttcgatcc catgaacgtc agcggcggca
     2461 tggatgcctg gaactcctcc ggctaccccc tcgcccaagc cagcttcagc gccggggctg
     2521 atgccgctag atagatgcgg cagcaccagg agacgaaacc gccacgacac caaggacgcc
     2581 aagagcgcgc caagtaaagt acggttcacc gcagagacgc agagagcctg ccctgagcgc
     2641 agccgaaggg acgcagagat aaaggaaggt ttactaaagg gtaagggcag catctactgt
//
LOCUS       DASRWZ010000131         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_80183,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000131 DASRWZ010000000
VERSION     DASRWZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80183"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..2100)
                     /locus_tag="VFH60_01905"
     CDS             complement(37..2100)
                     /locus_tag="VFH60_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6312563.1"
                     /translation="MYSGSEGSTPMMEGGTLLADRYRLLEKVGEGGAAEVFRARDQRL
                     DRTVAIKLLRPQFSYDEPSRKRFVIEAKTAAGIAHPNIVDIYDFGETDDGSMFIAMQF
                     VTGANLKDVLQKRGRLSSAETISIVRQACSALHAAHERGLIHRDVKPQNILIDRQGHV
                     HLTDFGIVKALSGPALTQSGMTFGTAAYMSPEQATGAPIKAAADLYSLGCVMYEMLCG
                     MPPFTGENPAIVAYKQVWEQPRPLHDCVAEVPPSLEAIVMRLLNKDPGRRYESAHDLV
                     ADLDALNVTSNQPTQAVPVSQTPALGTGVAPHVPLAAELGQPVQMPAAPAPVPPARVS
                     ISQPTVSPPHPQSTVPTPVGPAQTRQVAPAAVSPYTAQPARSVQVNVSQRRRGLGMWP
                     AVLALLAGLLLCGLGAWQGSNILGMFGGGNNGTPAPTTLAQGVPTGTSQAGPIVADTP
                     SPEPPPTDTPQLSPTADTSMLPTATAIALLPTDTPVPPPTDTPAPPPTDTPAPPPPTD
                     TPLPEPAPTDTPIVFPTDTPEEVVEPTPVQAGGTGSVTLEDSNFRGGYFRQDGLYHGR
                     TATWVYGLGTAYSSMAASFVVESAPEGQALLSIVGLDSEDADKTPVSIMVNDVEIFVG
                     GNPLPNDFIGSPTGPGNWGTAQLVIPAGLLQPGVNTLRITNLSPSARINAPPFFMLDS
                     ATITW"
BASE COUNT          361 a          699 c          900 g          459 t
ORIGIN      
        1 gtaattcgta attcgtaatt cgtaattcgt aatttcctac caggtgatcg tcgcgctgtc
       61 cagcatgaag aaggggggcg cgtttatcct ggcgctgggg gagaggttgg tgatgcggag
      121 ggtgttgacg ccgggctgca agagaccggc gggtattacc agttgcgcgg tgccccagtt
      181 gccggggccg gtggggctgc ctatgaagtc gttgggcagc gggttgccac ctacgaatat
      241 ctctacgtcg ttcaccatga tgctgacggg cgtcttgtcc gcgtcttccg agtcgaggcc
      301 aactatgctg aggagggcct ggccctcggg cgcgctctcg accacgaagc tggccgccat
      361 gctgctgtag gcggtgccca ggccgtacac ccaggtggcc gttctcccgt ggtacaggcc
      421 gtcctggcgg aaatagcccc ctctgaagtt actatcctcc agcgtgacgc tcccggtgcc
      481 gcctgcctgc acgggcgtcg gctctacgac ctcttcgggc gtatcggtcg ggaacacaat
      541 cggcgtgtcc gtgggcgcgg gttccgggag gggtgtgtcg gttggaggtg ggggcgcggg
      601 cgtatcggtg ggtggtgggg cgggcgtgtc ggttggaggt ggcacggggg tgtcggtggg
      661 cagcaaggct atcgccgtgg cggtcggcag catggacgtg tccgcggtag gcgatagctg
      721 gggcgtatcg gttggaggcg gctccggcga cggggtgtct gcgacgattg gcccggcctg
      781 cgacgtgccg gtgggcacgc cctgggcgag cgtggtggga gcgggcgtgc cgttattgcc
      841 cccgccgaac atgcccagga tgttactccc ctgccaggcc ccgaggccgc acagcagcaa
      901 ccccgccagc agcgcgagca ccgcgggcca catgcccagc ccgcgcctcc tctgggagac
      961 gttcacctgc acgctcctgg caggttgcgc cgtgtagggt gagactgccg ccggggccac
     1021 ctgcctcgtc tgtgccggtc ccacaggcgt gggcaccgtg gactgcgggt gcggcggtga
     1081 gacggtgggt tgggatatgc tcacgcgggc gggcggcacg ggggccgggg cggcgggcat
     1141 ctgcacgggc tggcccaact cggccgctag gggcacgtgc ggcgctacgc cggtccccag
     1201 cgcgggggtc tgcgagacgg gcacggcctg cgtgggctgg ttggaggtca cgttcagcgc
     1261 gtcgaggtcg gccacgaggt catgggccga ttcgtagcgt ctgccggggt ctttgttgag
     1321 cagccgcatg actatggctt ccagcgaagg cggcacctcg gccacgcagt cgtgcagggg
     1381 gcgcggctgc tcccacacct gcttgtaggc cacgatagcg gggttctcgc cggtgaaggg
     1441 cggcatgccg cacagcatct cgtacatgac gcagccgagc gaatagaggt cggcggcggc
     1501 cttgatggga gcgccggtgg cctgttctgg cgacatgtag gccgccgtgc cgaacgtcat
     1561 gcccgactgg gtgagggcgg ggccggaaag ggctttgacg atgccaaagt cggtcaggtg
     1621 gacgtggcct tgcctgtcaa tcaggatgtt ctggggcttc acgtcgcggt ggatgaggcc
     1681 gcgctcgtgg gcggcatgca acgcgctgca cgcctgccgg actatggaga tcgtctcggc
     1741 ggagctaagc cgcccgcgct tctggagcac gtccttgagg ttagcgcccg taacgaactg
     1801 catcgcgata aacatcgagc cgtcgtcggt ctccccgaag tcgtagatgt ccacgatgtt
     1861 ggggtgtgct ataccggcgg cggtcttggc ctcgattacg aatcgcttgc gggaaggctc
     1921 gtcgtaggag aactggggcc tgaggagctt gatagccacg gtgcggtcga ggcgctggtc
     1981 cctggcgcgg aatacctcgg cggccccacc ctcgcccacc ttttcgagca ggcggtagcg
     2041 gtcggcaagc agcgtgccgc cttccatcat gggtgtagag ccttcagaac cggagtacat
     2101 ggaccacctg ctcctcacat gtcgcaaacg gtgacgccgg attgggcacc gagcgtcaac
     2161 ggggtaccaa tagttaaacg atgcccccag gccggatgtc acgccagccg gggatgttgg
     2221 tgctttatgc ggtgccgctc tttcttataa gcattaaccg cgccgtgctt ggagaagagg
     2281 agtggtcagt tgttagttgg cgttggcaat gtgggtgccc ggcatggacg ccctgtggct
     2341 tatgaagatt aactggttaa tcaggtaatg gccaggcacg aatgggtgct gcgtaggggc
     2401 aggcccccgt gcctgccct
//
LOCUS       DASRWZ010000132         5143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_970866, whole genome shotgun sequence.
ACCESSION   DASRWZ010000132 DASRWZ010000000
VERSION     DASRWZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970866"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..869)
                     /locus_tag="VFH60_01910"
     CDS             complement(75..869)
                     /locus_tag="VFH60_01910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6312564.1"
                     /translation="MGLDAHIQNYLRGAASRGREVERIGPFLATFSPGDDNPYLNYAI
                     PDDGARPSSEDVSALVAAYRRHNRTPRLEYLPGLAPDVEAALLEGGLVVEGRPPLMVY
                     EPGTEREVPVPAGIELVVPTTDEDLWGMLVAQNEAYGGSAPTVEQVESQRAFLAAGGL
                     GILAREAGTGEPAGAGGCTVPANGTTELVGVGVRDKFRRRGIAAAMTAHLVRDALSVG
                     VTTVFLMAAHEAEARIYARAGFSQVGEVLHISLPGRLNHATGGMGE"
     gene            complement(871..2682)
                     /locus_tag="VFH60_01915"
     CDS             complement(871..2682)
                     /locus_tag="VFH60_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6312565.1"
                     /translation="MKTWHVMGRTIGFRFWLYMLNMVIWTLFFGAPLVMGLVMKAFFE
                     ALSGGAQAGGTIWTLVAFSVVVGIISMAALWVGIDQWSVFWYSSHALLRKNMMQWLVS
                     GPGARVLKEAPGETVNRFRDDVEHVVKYIDNWFDSTGQGLVTLVAVAVMFSINWGITA
                     LVLLPLMIVIMAANMLSAKLKKYRQLNREATARVTGAIAEMFGGIQAIKVASAEEDVL
                     AHLRTLNEKRRKVALMDSLITQLVDSFNANTTNIGTGLVVIVAAESFRARSFTVGDFA
                     LFVTYMGIVTSIPRWVGSLWAKYKQTGVSIDRMSSVMEGTTPNDLVAGGPIYFYQDAP
                     APEFAPKTAQHTLRGLEVRGLSYKYPGSQRGIEDVSFTVERGSFTVITGRIGSGKTTV
                     LKALLGLVPADSGEITWNGERVEDPASFLVPPRTAYTPQVPRLFSETLRDNVLMGLPP
                     EQVDLDAALHLAVMERDIAAMEGGLDTLVGPKGVRLSGGQVQRAAAARMFVRDAELLV
                     FDDVSSALDVETERVLWQRLFERREATCLVVSHRRAALRQADNIIVLKDGKVEASGTL
                     DDLLLTCDEMKLLWHGHAGYADEDVAVETEQEEPVAV"
     gene            complement(2679..4424)
                     /locus_tag="VFH60_01920"
     CDS             complement(2679..4424)
                     /locus_tag="VFH60_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6312566.1"
                     /translation="MKIPLKQYGQFLGKYLRTQLPAVLLLAFVLFTNIGLQLWNPLIL
                     KDFVDTAGKGVGLDSLVGPGVLFVSIALASQLLTVLATYLGESVGWNATNQLRVDLAL
                     HCLRLDMPFHKTHTPGEMIERIDGDVSAMSNFFSTFVIKVLGNILLMFGVLVVLFLED
                     WRIGVTLGLFVALMLFSMLRMNGFAVVPMRDEREASAGLFGFIEERLSGLNDIRANGA
                     GGYVMQGLYRSMRTQFTTGRRAWRADGTLWSTFVGLFTLGHILAVAVSAWLFLSGSIS
                     FGAVFLVFFYAGMLDRPMGELTEQLKDLQKAGAGLVRVQELLGIPVDIVDGRGTGFPR
                     GALAVSFEDVSFGYGDEEMVMHALSMHLKPGTVLGLLGRTGSGKTTLARLLLRLYDPV
                     EGAISLNGVDIRHARLRELRHAVGFVTQDVQLFNATLRENLTMFDSAIPDAKIIQVVH
                     ELGLSEWYAALPDGLDTVLGPGGGSLSAGEAQLLAFARIFLRDPGLVVLDEASSRLDP
                     ATERLIEQAVDRLLRGRTGIIIAHRLATVQRADEIMVLEDGRVLEHGPRLELANNPRS
                     RFSQLLKTGLEEVLA"
     gene            complement(4510..>5143)
                     /locus_tag="VFH60_01925"
     CDS             complement(4510..>5143)
                     /locus_tag="VFH60_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6312567.1"
                     /translation="DGQSLDSGLDPEMLVKVGRYMAELHNHSESFAPPAGFQRPRWDR
                     NRLFGANSVLHTPDAGRYFSRSELKLFEAAAERVSRTMQRLGEGREVFGLIHADLHNR
                     SYLFHGDQVGAIDFSDCGWGYYLYDIAIALGELKHRDDYPALHAAFLSGYRQVRPLSE
                     QHEGLINTFMVARRVDLVNNILGWEDPFLLPWVPRFLEKSIEVLKRYLGN"
BASE COUNT          911 a         1718 c         1592 g          922 t
ORIGIN      
        1 tggcccagag ccaccggcct atctccggct cgtagccagg cagtggcagc acatctcgca
       61 tgtcctgaga gtcgttactc acccattcct cctgtagcgt ggttcaatct gcctggcagt
      121 gagatatgta agacctcccc gacctgggag aaacctgcgc gagcgtagat gcgcgcttct
      181 gcttcgtggg ccgccatgag gaacaccgtc gtcacgccta cggagagtgc atcacgcacc
      241 agatgcgcgg tcattgcggc tgcgataccc cggcggcgga acttatcgcg cacgcccacc
      301 cccacaagct cggtggtgcc attggccggt acggtgcacc caccggcccc cgcaggctcg
      361 cccgtgcccg cctcccgcgc caggatgcca agcccacctg ccgccaggaa ggctcgttgc
      421 gattctacct gctcaacggt aggcgcggag cctccatatg cctcgttctg cgcgacgagc
      481 ataccccaca ggtcctcgtc ggtggtgggt acgaccagct cgatacccgc cgggaccggg
      541 acttcccgct cggtgccagg ctcgtatacc atgagcggtg gccgtccttc aacgaccaat
      601 ccgccctcaa gcagggcagc ctccacgtcg ggtgccaggc caggcaggta ctccaaccgt
      661 ggagtgcggt tatgcctccg gtaagcggca accagggcag acacatcctc agatgacggc
      721 cgggcaccgt catccgggat ggcatagttg agatacgggt tgtcgtcgcc cggagagaag
      781 gtcgccagga aggggccgat cctctcgact tcgcgcccgc ggctggctgc tcctcgcagg
      841 tagttctgga tgtgggcatc cagtcccata ttagaccgcc accggctctt cctgttccgt
      901 ttccaccgcc acatcctcgt ccgcgtagcc cgcgtgcccg tgccacagca gcttcatctc
      961 gtcgcaggtg aggagcaggt cgtccagggt gccgctggcc tctaccttgc cgtccttgag
     1021 cacgatgatg ttgtctgcct gtcgcaaggc cgccctccgg tgggagacga ccaggcacgt
     1081 agcctccctg cgctcgaaca gccgctgcca cagcacgcgc tccgtctcca cgtccagcgc
     1141 gcttgagacg tcgtcgaaca ccagcagctc ggcgtcgcgc acgaacatcc gggcggcggc
     1201 ggctctttgc acctgcccgc ccgacagcct cacgcccttg gggcctacca gcgtatcgag
     1261 gccgccctcc atcgcggcga tgtccctttc catgacggcc aggtgcaacg cggcgtcgag
     1321 gtctacctgc tcgggcggga ggcccatgag gacgttgtcc ctgagcgtct cgctgaacag
     1381 gcggggcact tgtggcgtgt aagccgtgcg gggcggcacc aggaacgagg cggggtcttc
     1441 cactcgctcg ccgttccagg tgatttcgcc gctatcggcg ggcacgaggc cgagtagcgc
     1501 cttcagcacg gtcgtcttgc cgctgccgat gcgccccgtt atcaccgtga acgagccgcg
     1561 ttccacggtg aagctcacgt cctctatccc gcgctggctg ccggggtact tgtagctcaa
     1621 gccgcgcacc tccaagccgc gcagggtgtg ctgggccgtc ttgggcgcga actcaggcgc
     1681 gggcgcatcc tggtagaagt agattggccc gcccgcgacc aggtcgttgg gggtggtgcc
     1741 ctccattacg gaggacatgc ggtcgattga cacgccggtc tgcttgtact tggcccacag
     1801 gctgcccacc caccggggga tcgaggtcac gatacccatg taggtcacga agagggcgaa
     1861 gtcgcccacc gtgaagctgc gcgcccggaa cgactcggcg gccacgatca ccaccaggcc
     1921 cgtgccgatg ttggtggtgt tggcgttgaa cgagtcgacc agttgcgtga tcaggctgtc
     1981 cattagggcc accttgcggc gcttctcgtt gagcgtgcgc aggtgggcga gcacgtcctc
     2041 ttccgccgag gccaccttga tagcctggat gccgccgaac atctcggcaa tggccccggt
     2101 cacgcgcgcc gtggcctccc ggttgagctg gcggtacttc ttgagcttgg cgctcagcat
     2161 gttggccgcc atgatgacga tcataagcgg cagcagcacc agggcggtaa tcccccagtt
     2221 tatcgagaac atgacggcca cggccaccag cgtcaccagc ccctgcccgg tggagtcgaa
     2281 ccagttgtcg atgtacttga ccacgtgctc cacgtcgtca cggaagcggt tgaccgtttc
     2341 gccgggcgct tccttgagca ccctggcccc cggaccgctc accagccact gcatcatgtt
     2401 cttgcgcagc agggcgtggc tggagtacca gaataccgac cactggtcga tacccaccca
     2461 cagcgcggcc atgctgatta tgcccacgac cacgctgaag gccaccaaag tccagattgt
     2521 gccccccgct tgcgccccgc cgctgagtgc ctcgaagaag gccttcataa cgaggcccat
     2581 gaccaggggt gccccgaaga aaagggtcca gatcaccatg ttcagcatgt agagccagaa
     2641 gcggaagcct atggtcctgc ccatgacatg ccaggttttc acgccagtac ctcctccagg
     2701 cccgttttca gcagttggct gaagcgcgac cggggattgt tagctagttc caggcggggg
     2761 ccgtgttcca gcaccctgcc gtcctcaagt accatgattt cgtcggcccg ctgcaccgtg
     2821 gcgagcctgt gcgcgatgat gatgcccgtg cgcccccgca gcagcctgtc caccgcctgc
     2881 tcgatcagcc gctcggtagc cgggtcgagc cgggacgatg cctcgtccag cacgaccagg
     2941 ccggggtcgc gcaggaagat gcgggcgaat gctagcaact gcgcctcccc cgccgacagg
     3001 ctgcccccac cggggccgag caccgtgtcc agtccatcgg gcagcgcggc gtaccactcg
     3061 ctcaggccaa gctcgtgcac tacctgtatt atcttcgcgt cgggtatggc gctgtcgaac
     3121 atcgtcaggt tctcgcgcaa ggtcgcgttg aatagctgca cgtcctgggt cacgaagccg
     3181 accgcgtgcc gtagctctcg caggcgggcg tggcggatgt caacgccatt caggctgatt
     3241 gccccttcaa cggggtcgta gaggcgcagc agcaggcggg ccagggtggt cttgccactg
     3301 cctgtgcgcc ccagcaggcc gagcacggtg cccggcttga ggtgcatcga cagcgcgtgc
     3361 atgaccatct cttcgtcacc gtagccgaac gacacgtcct cgaagctaac cgcgagcgcg
     3421 ccgcgtggaa agccggtccc ccggccgtcc acgatgtcta cgggtatgcc aagcagttcc
     3481 tgcacgcgca ccaggccagc cccggccttt tgcaagtctt tcagttgctc ggtaagctct
     3541 cccatgggcc ggtccagcat gcccgcgtag aagaagacga gaaacacggc gccgaacgag
     3601 atgctgccag agaggaaaag ccacgcgctc accgcaaccg ccaggatgtg gcccagcgtg
     3661 aacaggccca cgaaggtgct ccatagcgta ccgtcggccc gccaggcccg gcggcccgtc
     3721 gtgaattggg tgcgcatgga cctgtagagg ccctgcatga cgtacccacc ggcaccgtta
     3781 gcgcggatgt cgttcagccc ggacaggcgt tcttcgatga agccgaacag ccccgccgag
     3841 gcctcgcgct cgtcccgcat gggcaccacc gcgaagccgt tcatgcggag catggagaag
     3901 agcatcagcg ctacgaacaa gcccagggtc accccgatgc gccagtcctc aaggaacagc
     3961 acgaccagca cgccgaacat cagcagtatg ttgccgagca ctttgataac gaacgtcgag
     4021 aagaagttgg acatggcgct tacgtcgccg tcgatgcgct cgatcatctc gccgggtgtg
     4081 tgggtcttgt ggaaaggcat gtcgaggcgc agacaatgca aggccaggtc cacccgcaac
     4141 tggttggtgg cgttccaacc gacgctctcg cccaggtatg tcgcaagcac ggtgagcaac
     4201 tggctggcca gggcgatgct aacgaaaagc acccccggcc ccaccaggct gtcaagcccc
     4261 actcccttcc cggcggtgtc cacgaagtct ttcaagatca gcggattcca gagttgcaac
     4321 ccgatattgg taaacagcac gaaggccagc aggagcaccg cgggcaactg agtccgcagg
     4381 tacttgccaa ggaactggcc gtattgtttt agggggattt tcatagttgt gctttcgggc
     4441 gtaacacgta acacgtaaca cgtagctctt tcttcttatg ccatacgcgt tacgcgttac
     4501 gtgttactct cagttcccaa ggtacctctt cagcacctcg attgatttct caaggaacct
     4561 gggcacccat ggcaggagga acggatcctc ccagcccagg atgttgttta ccagatccac
     4621 gcggcgggcg accatgaagg tgttgattag cccttcatgc tgctccgaga gcgggcggac
     4681 ctgccggtag ccgctcagga aggcggcgtg cagggccggg taatcgtccc ggtgcttcaa
     4741 ctcgcccagc gcgattgcga tgtcgtacag gtagtagccc cagccgcagt cgctgaagtc
     4801 gatggccccc acctggtcgc cgtggaacag gtagctgcgg ttgtgcaggt cggcgtggat
     4861 caggccgaac acctcgcggc cttcgcccag gcgctgcatc gtcctgctta cgcgctcggc
     4921 ggcggcttcg aacagcttta gctcactgcg gctgaagtag cggccggcat ctggcgtgtg
     4981 cagcaccgag ttggcaccga agaggcggtt gcggtcccag cgtgggcgct ggaacccggc
     5041 gggcggcgcg aagctttccg agtgattgtg cagttcggcc atgtagcggc cgaccttgac
     5101 cagcatctca gggtccagcc cggagtcgag cgactgtccg tcc
//
LOCUS       DASRWZ010000133         4692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1313768, whole genome shotgun sequence.
ACCESSION   DASRWZ010000133 DASRWZ010000000
VERSION     DASRWZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313768"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="VFH60_01930"
     CDS             complement(<1..1797)
                     /locus_tag="VFH60_01930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6312568.1"
                     /translation="MTRAPATLVANETQSRPYGPAGASRSSSALRFLPLVIGVVAFGV
                     FMLTATGFHTYDGISYIRDMGKPLPALVLPHHLVYEPTMLLVFNLWRALGYTGYADLP
                     GEMLSSLAGAGGLALFFSIARRCTQPSAVAVTATLVLGLTYGYWFYSVEVDVYVLPLF
                     FLLLAAWLYTRALERSAPAMFMVCLVGATHAVGVLYHQAALFVVPAFTLGIYLLPGSV
                     SGRVTRVVGYGVSLALVLVPAYFVGGVVVAGQDTPDKFVRWANNYGQLGTWGALTQDT
                     PANAISGASAAISSDYWVGRLLLLALAAVLVWRGRSGTRRGGPLAWLLWAWVGIYTVF
                     FTWWQPEVLKFWVLVLPPALLLVLLGFRWDVHAPRYRLAATIVVLLLAISNAPTIWAK
                     RDPMSDPGRRTSAALAQLSAPEDLIVLQSGSAEHYLPFMYDRINVMSTRELWYQAGQG
                     GEEQAVAGIRQRMWHALAKGSSVWLENRVLSPGVRTSDHYVFSPEQIGLLLSPYGTRI
                     NREHVAAGDSNFMRLSPREVFSPADAWTFTTDQTGWSGVNVLGEAVGEQGWCFHPAQD
                     PNLYGPPVRLQAANYSALSVTMTAGKTDRGQFF"
     gene            complement(1881..2288)
                     /locus_tag="VFH60_01935"
     CDS             complement(1881..2288)
                     /locus_tag="VFH60_01935"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET6312569.1"
                     /translation="MPRYYTLSEANEMLPQLSQLIELMQAQRRQLELLQGRSKVVAQK
                     TRGNGNHNPGEDVALAQATKQVDDALQAAIKQLQDWGIELKDLQTGLIDFPALREGRV
                     VYLCWQLGEPEIAYWHETDTGFAGRQPLDDQFT"
     gene            complement(2340..2780)
                     /locus_tag="VFH60_01940"
     CDS             complement(2340..2780)
                     /locus_tag="VFH60_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6312570.1"
                     /translation="MSVAGFKFIDLPTAAERLGVTRTELLKWVDEGRIRPFSGKGQQS
                     VFRTGDVEKLVVELGVAARAVEAQAQEASEQAGTPARARRRDPVKLLGTRLSQTSRWA
                     EITDQDIATWIDALEPVQYERVRKVVDIAVDRLEKVRASIEQRR"
     gene            complement(2832..3452)
                     /locus_tag="VFH60_01945"
     CDS             complement(2832..3452)
                     /locus_tag="VFH60_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6312571.1"
                     /translation="MKVSEVMIDKPPVVTPGDTISQAARLIDELHTSLVPVVDNQEDR
                     HVVGVITDKEVMRCTASGENPQASYVKDYMGNTYETVRVDQDVETALVEVPESVDAPK
                     GDLGSGTGTDSILGTPGGGGLMGRRPIVVVDDGRRVVGLITRPHLAMDMAKVQSGQVV
                     AGRTESMQLVWRCTDCGYLLTRAEQLPEQCPDCGAPKENFVLVTED"
     gene            complement(3621..4304)
                     /locus_tag="VFH60_01950"
     CDS             complement(3621..4304)
                     /locus_tag="VFH60_01950"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6312572.1"
                     /translation="MIVEQAAIKSRLDGLRLSYEGFTKQLARPGVSAERSERVTAELE
                     MLGEEIATLEKVHQLGRVEADRARIEAIVEERLEIVQAKLTEDVRFSGIEPESLGAVS
                     GEARALLWTLGRDRLTLAMQDMSQSMPVRDPGRTDRAVPNILLHALQEAPDAESRASA
                     AYELGKLQLAEAIPTLAAALDDEDAFVADVALQALGYFPEEVLREAQLSPATVERVTR
                     ARERRQQPA"
     gene            4425..4637
                     /locus_tag="VFH60_01955"
     CDS             4425..4637
                     /locus_tag="VFH60_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312573.1"
                     /translation="MSQDVGKRSSRRAISWAAVGVGAILLLLVPLYLVFNAVSGSTQV
                     TVGGTWLILSVVAGMGLVAAGAALAD"
BASE COUNT          844 a         1483 c         1512 g          853 t
ORIGIN      
        1 gaagaactgc ccccgatcgg ttttgcccgc agtcatagtc accgacaagg cgctgtaatt
       61 ggcggcttgc aggcggacgg gcgggccgta caggttcggg tcctgcgccg ggtggaagca
      121 ccaaccttgc tcgccaaccg cctcccccag gacgttaacc ccgctccaac ccgtctggtc
      181 cgttgtgaag gtccaggcgt ccgcggggct gaatacctca cgtggcgaca ggcgcatgaa
      241 gtttgaatcg cccgccgcca cgtgttcgcg gttgatacga gtgccatacg gggagagcag
      301 gaggccgatc tgttcaggcg agaaaacgta atggtcgctg gtgcggacgc cgggggagag
      361 tacgcggttc tcgagccaga cggaggagcc ttttgccagg gcgtgccaca tgcgctggcg
      421 gatgccggca accgcctgct cctcgccacc ctgacccgcc tggtaccaca actctctcgt
      481 tgacataacg ttgatgcggt cgtacatgaa cggcaggtag tgctccgcgc tgcccgattg
      541 caggacgatg aggtcttcag gcgcggatag ttgcgccagc gccgcgctcg tccgtcgtcc
      601 ggggtcggac atcgggtcgc gcttggccca gatggtcggt gcattggaga tggctagcag
      661 caacaccact atggtagccg cgaggcggta ccgtggcgca tggacatccc accggaagcc
      721 cagcaggacg agcagcaggg cgggcggcag cacgagcacc cagaacttca gcacttccgg
      781 ctgccaccag gtaaagaaga cggtatagat gcccacccag gcccacagca gccaggcgag
      841 cggaccgccg cggcgagtgc ccgaacgccc ccgccatacc agcacggcag cgagcgcgag
      901 caacagcagg cggccaaccc agtagtccga gctgatagcc gccgaagcac cgctgatggc
      961 attggccggt gtgtcctgcg ttagcgcgcc ccacgtgcca agctggccgt agttgttggc
     1021 ccaacgcacg aatttgtcgg gagtgtcctg gcccgcgacg accacgcccc cgacgaagta
     1081 ggcaggcacg aggaccaatg caagtgaaac cccgtagcct actactctag tgaccctccc
     1141 gcttacgctt cccggcagca ggtagatacc cagcgtaaag gccggcacga cgaaaagggc
     1201 ggcctggtga tagagcaccc caacggcgtg agtggcccct acgaggcata ccatgaacat
     1261 tgccggggca ctgcgctcca gagcgcgggt gtataaccag gcggccaaca gcaggaagaa
     1321 gagcggcagg acgtaaacat ctacctcgac gctgtagaac cagtagccgt acgtcagccc
     1381 cagcacgagg gtggcagtca cggcaactgc gctcggctgg gtgcaacggc gggctatgga
     1441 gaagaagagg gctagaccgc ccgcccccgc caggctgctc aacatctcac cgggcaggtc
     1501 ggcatagccg gtgtagccaa gcgcccgcca caggttgaac accaggagca tggtaggctc
     1561 gtagacgagg tggtgtggca ggaccagcgc ggggaggggc ttacccatgt cccggatgta
     1621 ggaaatgccg tcgtaggtgt gaaagccggt ggcggtgagc atgaacaccc cgaaggccac
     1681 aacaccaatt acgagtggca ggaagcgaag ggctgaggag gagcgggacg ccccggcagg
     1741 gccatagggt cgtgattgcg tctcgtttgc gacgagcgtg gcaggggcgc gtgtcatggg
     1801 gtggcgctgg acctcatacc cggctctgga agtggagttt ggaagcggct tgtatagatg
     1861 ggtgccgccg gcaaacgcat ctaggtaaac tggtcgtcaa ggggctggcg gcccgcgaag
     1921 ccggtatcgg tctcgtgcca gtaggcgatc tcaggctcgc ccaactgcca gcacaggtaa
     1981 acgacgcgcc cttcacggag cgcggggaaa tcaatcaggc cggtctggag gtccttgagc
     2041 tcgatgcccc agtcctggag ctgctttatc gcggcctgga gagcatcgtc tacctgcttg
     2101 gtggcctggg ccagcgccac gtcttcgccg gggttgtggt tgccgttgcc gcgtgtcttc
     2161 tgcgcgacca ccttcgacct gccctgcaga agctccagtt gccgccgctg ggcctgcata
     2221 agctcaatta gctgagacaa ctggggcagc atctcattgg cttcgcttag cgtgtagtag
     2281 cgcggcatct gctctccgtc ccgcccttca tgggaggtcc gctgcgaggg cggactgctt
     2341 caacgtcgct gctcgatgct ggcgcgcacc ttctcaagcc ggtccaccgc gatatcgacc
     2401 accttgcgta ccctttcgta ctgcaccggc tcgagcgcgt ctatccaggt ggcgatatcc
     2461 tggtcggtaa tctcggccca acgggacgtc tggctcagcc gcgtgcccag tagcttgacg
     2521 gggtctcggc ggcgtgcccg ggcaggcgtc ccggcttgct cgctcgcctc ctgtgcctgc
     2581 gcttccaccg cccgcgctgc gaccccaagc tctacgacca gcttctccac gtcccccgtg
     2641 cgaaacacgc tctgctggcc cttgccgctg aaggggcgga tgcggccctc gtccacccac
     2701 ttcagtagct cggtgcgagt cacgcccagg cgttcggcgg cggtgggtaa atctatgaat
     2761 ttgaaacctg caactgacat atagtgaagc ccggagggtt acatcggcca tgcggtcgcg
     2821 tgcgccaggt gctagtcctc ggtaacgagc acgaagttct ctttgggcgc tccacaatcg
     2881 gggcactgct ccgggagctg ctcggccctg gtaagcaggt agccgcagtc ggtgcaacgc
     2941 cacacaagct gcatagactc ggtgcggccc gccacgacct gccccgactg caccttagcc
     3001 atgtccatag cgaggtgcgg gcgggtaatg aggcccacca cacgcctgcc gtcgtccacc
     3061 accacaatgg gacgcctgcc catcagcccg ccgccaccag gggtgccgag tatagagtcg
     3121 gtgccggtgc cgctgcccag gtctcccttg ggtgcgtcca cgctttccgg cacctccacc
     3181 agggccgttt ctacgtcttg gtccacgcgc accgtctcgt aagtgttgcc catgtaatct
     3241 ttgacatagg aggcctgggg gttttcgccg gaggcggtgc agcgcatcac ttctttatcg
     3301 gtgatgacgc cgaccacgtg ccggtcttcc tggttgtcca ccacggggac gagggaggta
     3361 tgcaactcat caatcagccg tgcggcctgg gagatagtgt cgccgggcgt gaccacggga
     3421 ggcttatcga tcattacttc gctaactttc atcagggcct cctaatgaga gacaattaga
     3481 cgcgctacga cgcggcaaaa gaaagtgaga caataatcag gcgggcgaaa tgccttacag
     3541 taagcaggtg gcgcgcaccg gggcgcttac tgcggcatta tatcacaggg gtttgggccg
     3601 ggcaagcgta gaatcgcccg tcaagcgggc tgctggcggc gctccctggc acgggtgacg
     3661 cgctccacag tggccgggct aagctgggcc tcccgcagga cctcttcggg gaagtacccc
     3721 agggcctgta gcgccacgtc cgccacgaaa gcgtcctcat cgtcaagcgc ggcggccagg
     3781 gtgggtattg cctcggccag ttggagcttg ccaagctcgt aggcggcgct ggcccgcgac
     3841 tcggcgtcag gggcttcctg gagggcgtgc aggaggatgt taggcacggc acggtcggtg
     3901 cgtcccgggt cgcggaccgg catgctttgg gacatgtcct gcatggctag cgtcagccgg
     3961 tcgcgaccca gcgtccagag tagcgccctg gcctcaccgg acactgcccc cagcgattca
     4021 ggctcgatgc cgctgaaacg cacgtcctct gtcaacttcg cctgcacgat ctccagtcgc
     4081 tcttcaacga tagcctctat cctggcgcgg tccgcctcga cccggcccaa ctggtggacc
     4141 ttttcgagcg tggctatctc ttcgcccagc atctcaagct cggccgtcac ccgctcggac
     4201 ctttcggcgg acacgccggg gcgggcgagt tgtttcgtga agccctcgta gctaaggcgc
     4261 aatccgtcaa gcctgctctt gatcgcggct tgttcgacta tcatgctctc tccggtagtg
     4321 gcaggcgcgt gctgccttca gcgtacccag cgtccaatcg tttgacgctc ccaacggcgt
     4381 gtgcaataat acctgcacca attatacact cagggaggct taatttgtct caagacgttg
     4441 gcaagcgttc ctctcgccgg gccatatcgt gggcggcggt gggagtgggg gcgatcttgc
     4501 tgctgctcgt gccgctctat cttgtgttca acgcggtctc cggcagcacg caggtcacag
     4561 tgggtggtac gtggctaatc ctgtcggtgg ttgcgggcat gggcctggtc gcggcaggtg
     4621 cggcactggc cgactgaacc caggttcgca agcccctgaa aggtagattc cttgctcgtt
     4681 acccctatat cg
//
LOCUS       DASRWZ010000134         5270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1039683, whole genome shotgun sequence.
ACCESSION   DASRWZ010000134 DASRWZ010000000
VERSION     DASRWZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039683"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1068
                     /locus_tag="VFH60_01960"
     CDS             268..1068
                     /locus_tag="VFH60_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6312574.1"
                     /translation="MPYFEHDGTQVYYELRGEGDPILALPGFSEDIGDLEELIRRLEP
                     DYRVIAADLPGSGRSGPIPRQYTPDFYREDAHTMSALLRHLGVERAHVAGFSDGGEVA
                     LLMAVHYPDRVRSLTVWGAAGTLGAGDIAPMLDAIYNMFDDPIEDLQGWRDYIKEQYG
                     EEAGRATLQSWATASKAILARGGDISLSRAHEIRCPALLISGEYDPFVTPEMTRDLAS
                     HIPNSEDEVFPGMGHSVHHEHPDGFVEAVLRWLELVKWQEDQERDSKG"
     gene            1155..2006
                     /locus_tag="VFH60_01965"
     CDS             1155..2006
                     /locus_tag="VFH60_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02587 family membrane protein"
                     /protein_id="HET6312575.1"
                     /translation="MSKSKEGSWQKELDDIAHGMSGAFLFGVPLLFTMEMWWIGNFTE
                     PLRRLILVGLAFGANLMLAYFAGLKEERSLFANVLQAVEALAIGVVASAVVLLVLNQI
                     KLGDSLHHMLGTITVQVLPLSIGASAANVLLSRGGDKLDEDGSSSNPGQWKAALLDLA
                     RTIGGAMFIGFSIAPTEEVPMLAGQLSPGQELALIALSLAITYLIVFVSGFSPRQEQE
                     QNPGPFQRPISETASSYIVALLVALGALLVFSQVDLSEPVTSIIAKTLVLGLPAAIGG
                     AAARVLL"
     gene            2003..2503
                     /locus_tag="VFH60_01970"
     CDS             2003..2503
                     /locus_tag="VFH60_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312576.1"
                     /translation="MSNDRDSDDRQARDGGGGGGGQQSGGQTRDREQGASGGKEKQER
                     TTAEWVSLFISVAIVLALVGGLVYQIFAGGNRPPTIEVKPLMEEIRLEGDSYYLSLDI
                     TNSGDRTAENVEVMLSLDSGEGDPETMQFQVQFLDGGETENQTVIFKNNPAEGELTHT
                     ASFHVP"
     gene            2536..3630
                     /locus_tag="VFH60_01975"
     CDS             2536..3630
                     /locus_tag="VFH60_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HET6312577.1"
                     /translation="MSTPDTTPATDPLYGDRLPQAQAVMERFISGLRLDRKVLILCDL
                     DVDGLGAGVVLWHLLTRRGVEPGLIEVQHPPKGENAFTPSTRGYVARAHPRALFVLDL
                     GVSSRIIMHEIPTLMVDHHRPSGLPAVEYVNCISGYDWDPVPTSSLLTYLLCNSRGEV
                     SDKAWVAAIGNLGDLGPLHPELDRAAREQKLKWLREATTLLNAAKRSSLYAGDASFRV
                     LRDANSAREIVEGEGADIETLRQCRQEVKQALDEAKRLAPRFSKTEKMALLEFSSPSR
                     VHPLLAQSWHGRLKGYIVIAANRGFLPNRVAFSARTSTSTNLIEFLARFRDALPPNEM
                     EYGYGHDQATGGVISAEAWEQLKRAMGLDE"
     gene            complement(3781..4650)
                     /locus_tag="VFH60_01980"
     CDS             complement(3781..4650)
                     /locus_tag="VFH60_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HET6312578.1"
                     /translation="MANTTSSVKGAARDAKQKAEDSSRGGWIEWLSRFGYAVRGVLYI
                     VIGILAVQVALGQGGDMADKDGAIAAIGNQTFGELLLVLVIIGLAGYSLWGFVRAFLD
                     PFDRGNGPKGLLQRAGYIVSAVVYGALIFPTVRFLLHAGDEQAPGGNATADFSAWLMS
                     HQPLGQWLLIIIGVVAFIGGLGQAWLAFSGDFQKDLKFREMSEDSRALARRVGRAGHA
                     ARGVVFAMIGFFIAKAAWDADPGQARGLDGTLLLLAQQPYGPLLLGAVALGLIAFGVY
                     SILCARWIKTSDK"
     gene            4983..5150
                     /locus_tag="VFH60_01985"
                     /pseudo
     CDS             4983..5150
                     /locus_tag="VFH60_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
BASE COUNT         1054 a         1682 c         1635 g          899 t
ORIGIN      
        1 tcggcgtaga gacaagcccc ctttgtcatc ctgtactccc ctttgtcagc ctgagcgtag
       61 cgaaggatca ggtgggtagg ccagagtcta caacattggg gatgctggcg gtaagaggaa
      121 cataggtgca agcataggaa gagtcaaaca cgagcggctg atccttcgct gcactcagga
      181 tgacaaggcc ggggctttgg tgctgccagt ccaaattagg ggctgcccaa cagggcatga
      241 taagcctcgt ccgcaaaaag ggacaaaatg ccatacttcg aacacgacgg aacacaagtc
      301 tactacgagc tacgcggcga aggcgacccg atactggccc tgcccggctt cagcgaggac
      361 ataggcgacc tggaggagct aatccgccgg ttggagcctg actaccgcgt gatcgccgcc
      421 gacctgcccg gctccggccg ctcaggaccg ataccgcgac agtacacgcc cgacttctac
      481 cgggaagacg cgcacaccat gtcggccctg ttgcgccacc tcggcgtaga gcgggcgcac
      541 gtggcagggt tcagcgacgg cggcgaagtg gctctgctca tggcggtgca ctaccccgac
      601 agggtgcgtt cgctcaccgt atggggcgcg gcaggcacat tgggcgcggg cgatatagcc
      661 cccatgctcg atgccatata caacatgttt gacgacccca ttgaagacct gcaaggctgg
      721 cgcgactaca tcaaggagca gtatggcgag gaagcgggcc gcgccaccct ccagagttgg
      781 gccacggcat caaaggccat cctggcgagg ggcggcgata tcagcctgtc ccgcgcccac
      841 gaaatccgct gccccgccct gctcatcagc ggcgaatacg acccgttcgt cacccctgaa
      901 atgacccgcg acctcgcctc ccacatcccc aactccgagg acgaggtctt tcccggcatg
      961 ggccacagcg tgcatcacga acaccccgat gggttcgtgg aagcggtgct gcgatggctg
     1021 gaactcgtga aatggcagga agaccaggag cgtgattcta aagggtgaag cgtgatgcgg
     1081 ccgggcaagt aacactgccg ctagttgcgc cgttctaaat cagtagccaa catcacgttt
     1141 cgcgtttcgg agccatgagc aagtcgaagg aagggtcctg gcagaaagag cttgacgaca
     1201 tcgcccacgg gatgagcggg gcgtttctgt tcggggtgcc actcctgttc acgatggaga
     1261 tgtggtggat cggcaatttc accgagccgc tacggcgact gatcctcgtg ggcctggcct
     1321 ttggcgccaa cttgatgctc gcctacttcg ccgggctcaa ggaggagcgg tcccttttcg
     1381 ccaacgtgct acaggccgtg gaagccctgg ctatcggggt ggtggcctcc gccgtggtgt
     1441 tgctggtgct gaaccagata aagctaggcg attcgctgca ccacatgctg ggcaccatca
     1501 ccgtgcaggt acttccgttg agcatcgggg cttcggcggc caacgtgctg ctctccaggg
     1561 gcggcgacaa actggacgag gacggcagct cctccaaccc cggccagtgg aaggccgcgc
     1621 tgctggacct cgccaggacg attggcggcg ccatgttcat cggcttcagc atagccccca
     1681 ccgaggaggt gccgatgctg gcggggcagc ttagccccgg acaggagctt gccctcatag
     1741 ccctgagcct ggcgatcaca tacctgatag tgttcgtgag cggcttcagc ccgcgccagg
     1801 agcaggagca gaatccaggt ccattccagc gccctatcag cgaaactgcc tcctcttaca
     1861 tagtcgcgct gcttgtggcg ttgggtgcgc tgctcgtctt cagccaggta gatttgagcg
     1921 agcctgttac cagcatcatc gccaagacgt tggtgctggg cctgcccgca gccataggtg
     1981 gcgcagccgc gagggtcctt ttatgagcaa cgaccgcgac agcgacgaca gacaagccag
     2041 ggacggcggg ggcggcgggg gcggccagca gtccggcggg caaacgcgcg accgggagca
     2101 aggcgcatcc gggggcaagg agaagcaaga gcgcaccacc gccgaatggg tcagcctgtt
     2161 catcagcgtg gctatcgtgc tggctctggt tggcgggctt gtctaccaga tattcgcggg
     2221 cggcaaccgg ccccccacca tagaggtaaa gccgctgatg gaggaaattc ggctggaagg
     2281 cgacagctat tacctctccc tggacatcac caacagcggc gacaggaccg ccgagaatgt
     2341 ggaggtgatg ctctccctcg atagtggtga gggcgacccc gaaacgatgc agttccaggt
     2401 gcagttcctc gacggcggcg agaccgagaa ccagacggtt atcttcaaga acaacccggc
     2461 ggaaggcgaa ttgacccata ccgccagctt ccacgtgccg taacatgccg gcctcgcccc
     2521 cttcacaccg ctgacatgag cacccccgat accacccccg caacggaccc gctttacggc
     2581 gaccgcctcc cgcaagccca ggccgtaatg gagcggttta tctccggcct gcgcctcgac
     2641 cgcaaggtgc tcatcctgtg cgacctcgac gtggacgggc tgggcgctgg cgtggtgctg
     2701 tggcacctgc ttaccaggcg tggggtcgag cccggcctga tagaggtgca acacccaccg
     2761 aagggggaga acgccttcac tccctccacc aggggctacg tagcgagagc gcacccacgc
     2821 gccctgttcg tgctcgacct gggcgtgtcg agccgcatca tcatgcacga aatacctact
     2881 ctcatggtag accaccaccg cccaagtggg ctgcctgccg tggagtacgt caattgtatc
     2941 tccggttacg attgggaccc ggtgcccacg tccagcctgc tgacctacct gctctgcaac
     3001 agccggggcg aggtgtcgga caaggcgtgg gtagccgcga taggcaacct gggcgatctg
     3061 gggccgctcc accccgagct tgaccgggcc gccagggagc aaaagctgaa gtggctgcgc
     3121 gaggcgacta ccctgctcaa cgccgccaag cgcagcagcc tgtacgcggg agacgcgtcc
     3181 ttccgggtgc tgcgggacgc aaacagcgcg agggagatag tcgagggcga aggcgcggac
     3241 atcgaaactc tgcggcagtg caggcaagag gtgaagcagg cgctggacga ggctaagcgc
     3301 ctggccccgc gcttcagcaa gacggaaaag atggccctcc ttgagttcag cagccccagc
     3361 cgcgtgcacc ccctcctcgc ccaaagctgg cacggcaggc tcaagggcta catcgtaatc
     3421 gccgccaacc ggggcttcct ccccaaccgg gtggccttca gcgcccgcac ctctacctcc
     3481 accaacctga tagagttcct ggcccggttc cgcgacgccc tccctcccaa cgagatggag
     3541 tacggctacg gccacgacca ggccaccggc ggggtaatct cagccgaagc ctgggaacag
     3601 ctgaagcggg cgatggggct tgacgagtaa tgagtgatga ggactgagga ctgagttaag
     3661 agacccaacc gcaaaggcgc aaagagcgca aaggacgcat taagaggatg agtggggctg
     3721 ttagtgccac tcagtcctca gtcctcagtc ctcagtcctc agtcctcatc actcattact
     3781 tcacttgtca ctcgtcttga tccagcgggc gcagaggatt gagtagaccc cgaaggcgat
     3841 gaggccgagg gcgacggcac ccaggagcag ggggccgtag ggttgttggg ccagtagcag
     3901 gagcgtgccg tcgaggcccc gtgcctggcc ggggtcggcg tcccaggctg ccttggcgat
     3961 gaagaagcct atcatggcga acactacgcc cctcgcggcg tgcccggcac ggcctacccg
     4021 cctggccagc gccctggagt cctcgctcat ctcgcggaac ttgaggtcct tctggaagtc
     4081 tccggagaac gccagccacg cctgtcccag acctcctatg aaggccacaa ccccgatgat
     4141 gattagcagc cactgtccca aaggctggtg cgacataagc caggccgaga aatcggcggt
     4201 ggcgttgccg cccggcgcct gctcatctcc cgcgtgtaaa aggaaccgca cggtggggaa
     4261 tatcaacgcg ccatacacca ccgcgctgac aatatagccc gctcgctgta ggaggccctt
     4321 ggggccgttg ccccggtcga aggggtcgag gaaagcgcgg acgaagcccc acagcgaata
     4381 gcccgccagg ccgataatca ccagcaccag caacagctca ccgaaagtct ggttgcctat
     4441 agcggcgatg gccccgtcct tgtccgccat atcgccaccc tgccccaatg ctacctgcac
     4501 ggccaggatg cctatcacta tgtacagcac cccccgcacc gcgtacccga acctcgatag
     4561 ccattctatc cacccgcccc tggacgagtc ttccgccttc tgcttggcgt cgcgtgccgc
     4621 accctttacg ctcgatgtgg tattcgccac ggtttgttac ctgctttcat gctcgattct
     4681 gtgccctcgc ccctggtaca gcaaataatg taaagggcac gtttcatgcc agcggcagta
     4741 acttcggcgt ggggaccagg ctgccacatg gcatataaga tgcaggcgcc agtcccgttg
     4801 aaaaaccccg gaggtgccta gatgggcgat gagaacaaga gcaagctcag cgtgtcgctc
     4861 cccgtctcga tgacattcgc gggggggtgt cacggcaacg gtagtggggg tgccggtgct
     4921 cggcaagctg gtggaagccg cttttcggag tacacgcgcg caaggacaag ggcaagcgcc
     4981 gggccgtcct gcgcctgatg gccctcagca tgacgttgtg cggcctcacc ggttggagcc
     5041 gcatctacct cggcgtgcac cacccctctg acgtgctggc gggctggata gcaggcacca
     5101 cctggctgct cacctgcggc ctggcctcga agtacatgtc ggaagcgtaa tgttgccttg
     5161 ctgtgcttcg gtgcgtgaat gttaccaggc atgcatagca ggaccaaact cccctttgtc
     5221 attctgagcg tagcgaagaa tctcgtcccc cagcacccaa gcgccacctt
//
LOCUS       DASRWZ010000135         5079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_80367,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000135 DASRWZ010000000
VERSION     DASRWZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80367"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..427)
                     /locus_tag="VFH60_01990"
     CDS             complement(17..427)
                     /locus_tag="VFH60_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6312579.1"
                     /translation="MPRIEAVGFEPFEVPQGTRLVRALEDNGVDILHRCGGFAKCTTC
                     RVEFIEGEPDRMTVAERDRLEAKGVLGEVRLSCQVECTHDMRLRPVNLFSQSDLSDPG
                     PQPEDHITPDPEWIEAPRQRQELVWDTGQAPLAP"
     gene            642..1613
                     /locus_tag="VFH60_01995"
     CDS             642..1613
                     /locus_tag="VFH60_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6312580.1"
                     /translation="MPDVITCGEMLIDFVSTVSGVTLVEAPAFEKAPGGAPANVAVGL
                     ARLGVSTGFVGKLGDDDFGRYLQSVLEGNGVDTSRLQFTSEARTALAFVSLREDGERE
                     FVFYRHPSADMLYAPHEVHVPYIARARVFHFGSITLGAEPGASATLHAAHVARENGLF
                     VSYDPNLRLNLWPSAEAAREGMLRGWEYANLIKISEEEAEFLSGTEDLEQAAQDLWHG
                     NLRLLAVTRGEQGATFFAPGKSGYVPGFPVRAVDTTGAGDAFLAGLLFSLYPNLDRLT
                     QGDLAEDELQQAVRFANAAGALATTRRGAIPALPTLEEVQRLLATGD"
     gene            2017..2487
                     /locus_tag="VFH60_02000"
     CDS             2017..2487
                     /locus_tag="VFH60_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET6312581.1"
                     /translation="MAIKTDQKIDQQLDPRVGEIDRELRARGHRFTPRRLMVVEVLSR
                     HEGHLTGEEILAQVRHRHPSTDKTTVYRTLELLSSLGMVVVTDMGSGRMEYELSSNPH
                     HHLICERCDTRIEVEDSFFEPLRDRLWKQYGFCTNLAHFALFGVCPACKAKEES"
     gene            2747..4885
                     /gene="ispG"
                     /locus_tag="VFH60_02005"
     CDS             2747..4885
                     /gene="ispG"
                     /locus_tag="VFH60_02005"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HET6312582.1"
                     /translation="MPNTYAYRRRKTREVMVGSVGVGGDNPIRVQSMTTTQTDDVEAT
                     LAQTIRLVEAGCEIVRITTPTSKDARALGEVKRRLVAMGLDVPLVADIHFNPAAAMEA
                     ALYADKVRINPGNFADTKVFAVKEYSEDQYEAELARIEEKFKPIVLRCKERGISMRIG
                     TNHGSLSDRIMNRFGDTPEGMVESAIEFAAIARKYDYHDLIFSMKSSNPKVMIEAYRL
                     LVARLDALGWDYPLHLGVTEAGNAEDGRIKSATGIGALLDDGLGDTIRVSLTEDPEYE
                     VPVAFALVKPYNQMLGSRVIDAGGQEIVHAQTLPDLRDPFHFVPRPTYEVATGAVKTG
                     GMQQTAVVASVDVQPGQDLFAAVADLAKRKGQNAIRPDIIRVPLSGLREIAPLADDWK
                     RRGEEEDPAAGKSALALIVDAGDNFKGAEQVAGKLAGIGAIGLQVGGAEEVDWQAYEA
                     CVNAAIEAHVPLWLQTYGGLLNFINWTEVLDGTATFEGVDLLLELAKRAHALGHRGLV
                     LSLRQDDPTPSYMIRAYRYLSTRLAELGRKYPVHLVTEAGDFGQPLAHDDSQDGWLIG
                     ASIKVGSLLCDGIGQSIELGGTGTADERVTLAFNILQAAGARSVKAEFVACPSCGRTL
                     FDLQETTERIKKSTGHLRGVKIAVMGCIVNGLGELADADFGYMGGAPGKVNLFVGKEC
                     VEKGVPTEEAVDRLINLIHKHGKWIEPVVR"
BASE COUNT          977 a         1597 c         1620 g          885 t
ORIGIN      
        1 gaccgtaaag gtgcgactag ggggccagtg gtgcctgccc ggtatcccat accagctctt
       61 gccgctgcct gggggcttcg atccattcgg ggtcgggggt aatgtggtcc tcgggctgcg
      121 ggccggggtc ggataggtcg ctctgactaa acaggttgac cgggcgcaaa cgcatgtcgt
      181 gggtgcactc gacctggcac gacaggcgca cctcccccag cacgcccttg gcctccagcc
      241 tgtcccgctc ggccaccgtc atcctgtccg gctcgccttc gatgaactcc acccggcagg
      301 tggtgcactt cgcgaagccc ccgcaacgat gcaatatatc tacgccgttg tcttccagcg
      361 cccttaccag gcgggtgccc tgcggcacct cgaacggctc gaaaccgaca gcttctattc
      421 ttggcatatc tcgatacctc ccttacttat gctcttgctt gtgttggcct gcttttcttt
      481 cttattctgc gcgccgcagg gagccaccgc cggctggccc ctgtaatacc ccttaggaga
      541 gcaagccctg tgccgggaag gagagggtgt gcgcgacgac gtgcgccgtg ccttataatg
      601 ggtgccacat gaccgcgtgc cgataaccgg cggaggagtt gatgcctgac gtaattacct
      661 gcggcgagat gctgatagac ttcgtttcca ccgtatccgg ggtgacgctc gtggaagcgc
      721 ccgcgttcga gaaggcaccc ggcggcgcgc ccgcgaacgt ggcggtgggg ctggcgcggc
      781 tgggggtgtc taccgggttc gtcggcaagc tgggggacga cgatttcggg cgctacctgc
      841 aatcggtgct ggagggcaac ggggtggata ccagccgcct ccaattcacc tcggaggcgc
      901 gcaccgccct ggccttcgtc tcgctccggg aggacggcga gcgcgagttc gtcttctaca
      961 ggcaccccag cgccgacatg ctctacgctc cgcacgaggt gcacgtgccc tatattgccc
     1021 gcgcccgcgt gttccatttc ggctctatca cgttgggagc cgagcctggc gcttcggcca
     1081 ccctccatgc ggcccacgta gcccgcgaga acggcctgtt cgtctcgtac gaccccaacc
     1141 tgcgcctcaa cctgtggccc tcggcggagg ccgcacggga gggcatgctg cgcggctggg
     1201 agtacgcaaa cctcatcaag atcagcgagg aggaggccga gttcctatcg ggcaccgagg
     1261 acctcgaaca ggcggcccag gacctctggc acggcaacct gcgcctgctg gcggtgaccc
     1321 ggggcgagca gggggccacg ttcttcgcgc cgggcaagag cggctacgtg cccggctttc
     1381 cggtgcgggc ggtagacacc accggcgcgg gcgatgcttt cctggcgggg ctgctcttca
     1441 gcctgtaccc caacctggac cgcctcaccc agggcgacct ggctgaggat gagctacaac
     1501 aggccgtcag gttcgcaaac gccgccggtg cgctcgccac tacccggcga ggggccatac
     1561 ccgccttacc caccctggaa gaggtgcaac gcttacttgc gacaggcgat tagaagctat
     1621 cgcgaaaatg acattttgga acatcggcac ccaggccccg tccttgtcat ttcgaacgta
     1681 gtgagaaatc tcgtccttca gcacccaagt atcacctttc ttacaccaca gaatcccgtg
     1741 agaaaagagg aacatcccgg tgtaggacga gatttctcac tccgttcgaa atgacagggg
     1801 ccgcctttgg gtgctgatat tccactatgt tatttttgat atagcttatt gccgttaccc
     1861 cgtccccgtt gcgtatacgc cgcccaaagc gcgaaccccg gcacccagtc cgcacatgcc
     1921 gttcagaaac gctgatggtc tgctcgccgg ggcgcgcgga ctgcagaata gcttcttcag
     1981 gcccttacac ccgccctttg gtataattga ctggatatgg caataaagac ggaccagaaa
     2041 atagaccaac agctagaccc cagggtgggg gagattgacc gcgagctgcg cgcgcgcggg
     2101 cacaggttca cgccccgccg cctgatggtg gtcgaggtgc tcagcaggca cgaggggcac
     2161 cttaccggcg aggaaatcct ggcccaggtg cggcacaggc acccctccac cgacaagacc
     2221 acggtgtacc gcacgctgga actgctcagc agcctgggca tggtggtagt caccgacatg
     2281 ggcagcggga ggatggaata cgaactgagc agcaaccctc accaccacct catctgcgag
     2341 cgctgcgaca cccgcataga ggtggaggac agcttcttcg agccgctacg cgaccgcctc
     2401 tggaagcagt acggcttctg caccaacctg gcccacttcg ccctcttcgg cgtctgcccc
     2461 gcctgcaagg ccaaagaaga gtcttgagtg ctgtaccttt aggggagaat agacgtatat
     2521 atagtaagtg atgaggactg aggactgaag actgagcaga gagactaaag cgccaagacg
     2581 caaggaacgg ctgagagtga taaggtctaa gatgtatagc actcgtcact cagtcctcat
     2641 cactcattac tcggcaaaca tgaggtaaca caaagtgagc ataacaattc cgggcggtga
     2701 gcctatcata ttgaaatcgg cggcggagac gcccaggcac ccttatgtgc cgaacaccta
     2761 cgcctacagg cggcgcaaga cccgcgaagt gatggtgggg agcgtcggcg tgggcggcga
     2821 caaccctatc cgcgtgcaat cgatgaccac cacgcagacg gacgacgtag aggccacgct
     2881 ggcgcagact atcaggctgg tggaggcggg ctgcgaaatc gtgcgcatta ccacccccac
     2941 ctcgaaggac gcccgcgcgc tcggtgaggt gaagcggcgg ctggtggcga tgggcctcga
     3001 cgtgccgctg gtggccgaca tccacttcaa cccggcagcc gcgatggaag ccgccctcta
     3061 cgccgacaag gtgcgcatca accccggcaa cttcgcggat acgaaggtgt tcgcggtcaa
     3121 ggagtacagc gaggaccagt acgaggccga gcttgcgcgc atcgaggaga agttcaagcc
     3181 gatagtgttg cgctgcaagg agcggggcat ctccatgcgc atcggcacca accacggctc
     3241 cctctccgac cgcattatga atcgcttcgg cgacaccccg gaaggcatgg tcgagtcggc
     3301 catcgagttt gcggcgatag cccgcaagta cgactaccac gacctgatct tctccatgaa
     3361 gtcgagcaac cccaaggtga tgatcgaagc ctaccggctg ctggtggccc gcctggacgc
     3421 gctgggctgg gactatccgc tgcacctggg cgtgacggag gcgggcaacg cggaggacgg
     3481 gcgcatcaag tcggccacgg gcataggcgc gctcctcgat gacggcctgg gcgacacgat
     3541 acgcgtgtcc ctcaccgaag acccggagta cgaggtgccg gtggccttcg cgctggtcaa
     3601 gccctacaac cagatgctgg ggtccagggt aattgatgca gggggccagg aaatcgtcca
     3661 cgcgcagacc ctccccgacc tgcgcgaccc cttccacttc gtgccccggc ccacctacga
     3721 ggtagccacg ggcgctgtga agaccggcgg catgcagcag acggcggtgg tcgccagcgt
     3781 ggacgtgcag ccggggcaag acctgttcgc ggcggtggcc gacctggcga agcgcaaggg
     3841 ccagaacgcc atccgccccg acatcatccg cgtacccctc tccggcctcc gggagatcgc
     3901 acccctcgcc gacgactgga agcggagagg tgaggaggaa gacccggcgg ctggcaagtc
     3961 ggccctggct ctcatcgtgg acgccggaga taacttcaag ggggccgagc aggtggcagg
     4021 caagctggcc ggtatagggg caatcggtct ccaggttggc ggggcggaag aggtagattg
     4081 gcaggcgtac gaggcgtgcg tgaatgccgc tatcgaggcg cacgtgcccc tgtggctgca
     4141 aacctacggc ggcttgctga atttcatcaa ctggaccgag gtgctggacg gtacggccac
     4201 gttcgagggt gtggacctgc tgctggagct ggcgaaacgc gcccacgccc tgggccaccg
     4261 gggtctcgtg ctgtccctcc ggcaggacga ccccacccca tcgtacatga tacgggccta
     4321 ccgctatttg tctactcgcc tggccgagtt aggccgcaaa tacccggtcc acctggttac
     4381 cgaagcgggc gactttggtc agcccctggc ccacgacgac tcgcaggacg gctggctgat
     4441 aggggcctcc atcaaggtag gctccctgct ctgcgacggg atagggcaga gcatcgagct
     4501 aggcggcacg ggtaccgcag acgagcgcgt gaccctggcg ttcaacatcc tccaggcggc
     4561 gggtgcgcgc tccgtgaagg ccgagtttgt ggcctgcccc tcctgcgggc gcaccctctt
     4621 cgacttgcag gagaccaccg agcgcatcaa gaagtccacc ggccacctgc gtggggtgaa
     4681 gattgcggtc atgggctgca tcgtcaacgg cctgggggag cttgcggacg ccgacttcgg
     4741 ctacatgggc ggcgcgccgg gtaaggtgaa cctgttcgtc ggcaaggagt gcgtcgagaa
     4801 aggcgtgccc accgaagagg ccgtagaccg cctcatcaac ctcatccaca agcatggtaa
     4861 gtggatcgaa ccggtggtga ggtgagttag gatttaggat tttgggggcg gtagctctac
     4921 aagcttcctg gatgtagtca ataccggcaa gcacactact ggggacggcc tgtatggacg
     4981 ccctgtggcg gtgccctgcc aatgaattgg cgggattacc agagacgaag tctccttcgg
     5041 agactaccct cacgttccct tagtccccgt aggggactt
//
LOCUS       DASRWZ010000136         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_628623, whole genome shotgun sequence.
ACCESSION   DASRWZ010000136 DASRWZ010000000
VERSION     DASRWZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628623"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..794
                     /locus_tag="VFH60_02010"
     CDS             153..794
                     /locus_tag="VFH60_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET6312583.1"
                     /translation="MKYYIAVAGNIGVGKSSLTELLAERLGWEPFYEAVAENPYLSDF
                     YQDMRRWSFHSQVFFLSRRLRHHYDLLNRPGSVIQDRSVYEDAEIFARNLFNGGNMSE
                     ADWRTYYDLYELATTIVRPPNLVVYLQASLSTLLGRIVLRGRDFERAIQPEYLAQLNE
                     LYEDWASRFNLCPVLTINTDGMDFVHNATHMEQIARRIRDRLQGKETITLQSP"
     gene            809..1678
                     /locus_tag="VFH60_02015"
     CDS             809..1678
                     /locus_tag="VFH60_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6312584.1"
                     /translation="MMQETIEYVEVPGATLWTARQGSGPALVMLHGGPGLWDYMGPLA
                     GMVDDLATVYRYDQRACGRSSGGPPFTVQAAVADLEALREHWGVERWVVLGHSWGATL
                     ALAYCLAHPGRVHALVYVSGVGIYTGWREEFHANMGALVPTEEKQQLADLRALLASGD
                     GEISAAANREYCVRTWSVEMLDRANARELAESVLVEGVQANWEVNRVLSSEGERFAEQ
                     GDMPERLAALRVPTLVLHGEADPRPAWAGRRVAEHIPGAEFVLLREAGHFVWLDNPGG
                     LRGALRRFLVGVL"
     gene            2098..2385
                     /gene="rpsO"
                     /locus_tag="VFH60_02020"
     CDS             2098..2385
                     /gene="rpsO"
                     /locus_tag="VFH60_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET6312585.1"
                     /translation="MALETERKQTIVSDYKVNDTDTGSPEVQVAILTTRINDLRAHFT
                     AHKHDHHSRRGLLKMVGRRRRLLSYLAKNDINRYRSLISRLGLRDATRTNS"
     gene            2586..>2974
                     /locus_tag="VFH60_02025"
     CDS             2586..>2974
                     /locus_tag="VFH60_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET6312586.1"
                     /translation="MREDKVYSATIGGREFTIATGKLAEQASGACTVRYGDTIVLAAV
                     VAAKEAMEGRDFLPLTVDYEERMYAAGKIPGGFIKREGRPTETATLTARLTDRPIRPL
                     LPKQWRSEIQVTITTLSSDQENEPAILS"
BASE COUNT          578 a          918 c          924 g          554 t
ORIGIN      
        1 acgaaaacgc aaagacgcaa agaacgcaaa gaacgcgcaa agaagattga ggttttggcg
       61 tagtgccact cggcactcgg cactcggcac tcggcactcg gcactcggca ctcgtctggc
      121 atcacccgct tacatctgct ataagtagca gcatgaagta ctacatcgcg gtcgcgggta
      181 acatcggggt gggcaagtcg agcctgacgg agctgctggc cgagcgtttg ggctgggagc
      241 ctttctacga ggcggtggcc gagaacccct acttgagcga cttctaccag gacatgcggc
      301 ggtggagctt ccattcgcag gtcttcttcc tgtcgcgccg cctccggcac cattatgacc
      361 tgctcaaccg ccccggctcg gtgatacagg accgcagcgt gtacgaggac gcggagatat
      421 tcgcccgcaa cctgttcaat ggcggcaaca tgtccgaggc cgactggcgc acttactacg
      481 acctctacga gcttgccacc acaatcgtgc ggccccccaa cctggtggtc tacctgcaag
      541 cctcgctgtc cactctgctg ggccgtatcg tgttgcgggg ccgcgacttc gagcgtgcga
      601 tacagcctga atacctcgcc caattgaacg agctttacga ggactgggcg agccgcttca
      661 acctgtgtcc cgtgctcacc atcaacactg acggcatgga cttcgtgcac aacgccacgc
      721 acatggagca gattgcccgc aggatacgcg acaggttgca gggcaaagag accattacgt
      781 tgcagtctcc atgagcaatc gggcaagcat gatgcaagag accatagagt acgtagaggt
      841 gccgggcgct acactctgga cggcgcggca ggggagcggg cctgcgctcg tcatgctgca
      901 cggcgggcca gggctgtggg actacatggg gccgcttgcg ggtatggtag acgacctggc
      961 gacggtgtac cgctacgacc agcgcgcgtg tggcaggtca tcgggtgggc cgcctttcac
     1021 ggtacaggca gcggtggccg acctcgaagc gttgcgcgag cactggggcg tggagcggtg
     1081 ggtggtgctc ggtcactcgt ggggggccac gctggccctc gcctactgcc tggcccaccc
     1141 tgggcgcgtg catgcgctgg tctacgtgtc gggcgttggc atctacacag gctggcggga
     1201 ggagttccac gccaacatgg gcgcgctcgt ccctacggag gagaagcagc aactcgcgga
     1261 cctgagggcg ctactggcaa gcggcgatgg cgagatatcc gccgcggcca accgtgagta
     1321 ctgcgtccgc acgtggtccg tcgagatgct cgaccgcgcc aacgcccgtg agcttgcgga
     1381 gagcgtgctt gtggagggcg tgcaggccaa ctgggaggta aaccgggtgc tcagctcgga
     1441 gggggagcgg tttgccgaac agggcgacat gccggagaga cttgccgccc tgcgcgtgcc
     1501 caccctggtg ctccacggcg aggctgaccc acgtcctgcc tgggcggggc ggcgtgtggc
     1561 ggagcacata ccgggtgccg agttcgtgct gctgcgggag gcgggtcatt ttgtatggct
     1621 ggacaaccct gggggtttgc ggggtgcgct ccggcgattt ctggtggggg tactctaggg
     1681 aatgccctct atgttgccct gtatggccgc cctgtggcta aagccacggg ctgggaagta
     1741 gcgaagtctc cttcggagaa taccctcacg ttcctcagtc cccgcagggg actttgcctc
     1801 ttctcagccc gtggctttag ccacagggct atcccagggc gtcccgtctg ccctatgcag
     1861 tccgcaaata cactctataa cctccctatg tctcgtaata caccgttagt cccctcaacc
     1921 ggcagtggct gcgcgaatcg gccttgctcc gcgcacttgg atgtgctata attctgggta
     1981 ttgccatgaa tggcggagag caaaagaggc aagagtaatt cgcctccccg cgatattaat
     2041 agcagcaagc cttatgaagt acggcgcacg cggttggcgc ataggaggac tagaactttg
     2101 gcactggaga cagagcgcaa gcagactatt gtcagcgact acaaggtaaa cgacaccgac
     2161 accggctcgc ctgaggtcca ggtagcgatc ctgacgaccc gcatcaacga tctgagggcg
     2221 catttcaccg cccacaagca cgaccatcat tcgcgccgcg gcctgctcaa gatggttgga
     2281 cgccgccgcc gcctgcttag ctacctcgct aagaacgaca tcaacaggta tcgcagcctc
     2341 atttcccgcc ttgggctgcg cgatgcgacc cgcaccaatt cgtaggcact ccgctttcag
     2401 tagcaagttg tcagttctca agtcagggct ggcaactttc tgctatctgc cgtcttacgc
     2461 atggcatatg catggcacgc cacgtgcacc acttttgcgt gaaagcatga acgggtagcg
     2521 tatcattcac tttagccggc ccgccgcaca atccggtgca ggcggcacac attaggagac
     2581 gtttcgtgag agaagataag gtatattccg caacgattgg cggacgcgag tttacgattg
     2641 ccaccggcaa gctcgcagag caggcgtctg gtgcctgcac ggtgcgctac ggcgacacca
     2701 ttgtactggc cgccgtggtc gccgcgaagg aagcgatgga gggccgcgac ttcctgcccc
     2761 tgacagttga ttacgaagag cgcatgtacg ccgccggtaa gattccaggc ggctttatca
     2821 agcgggaagg ccgccccacc gagacggcca ccctcaccgc ccgcctcacc gaccggccca
     2881 tccgccccct cctccccaag cagtggcgct ccgagataca ggtgactatc accaccctct
     2941 ccagcgacca ggagaacgag cctgctatcc tctc
//
LOCUS       DASRWZ010000137         1919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_902914, whole genome shotgun sequence.
ACCESSION   DASRWZ010000137 DASRWZ010000000
VERSION     DASRWZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902914"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1136
                     /locus_tag="VFH60_02030"
     CDS             24..1136
                     /locus_tag="VFH60_02030"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET6312587.1"
                     /translation="MMLGDLGAEVIKVESPEGDDTRRWGPPYQGSESAYYLCCNRNKR
                     GIVLDLGRPVDRETARELGIRSHVVVENFRLGTMEKWGLSYEALSAENPGLVYCSISG
                     YGRTGPDAHLPGYDYVVQGAGGIMSVTGEPDAPPAKVGVAIVDLTAGMFACSAILAAL
                     RVRDLTGRGQRIDISLLDSHLAWLANVGSNYLISGEVPARYGNGHANIVPYQTFATSD
                     GWVVLATGNDRQWQRFCMAIERPDLLADPRFATNHARVEHRGMLVPMLEAIFLTRTTH
                     EWVALCAGADVPAGPVNTVDRALNSPQALARDMVRSVDHPGIGPVRMVASPLKLESTP
                     PTIRRHPPMLGEHTEEVLREVLGASVAQSPASGGGE"
     gene            1133..>1919
                     /locus_tag="VFH60_02035"
     CDS             1133..>1919
                     /locus_tag="VFH60_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312588.1"
                     /translation="MRRHPRLDAWMLGLKRVRSPRRANSRIRHMRPQVARAVRLLRAA
                     SRLGVPGSVPAPPELLPAGARALAQGYLNRFSMPLLNGWLLPPWMRRQSDPASPLFTP
                     RSVSNLMLNQTARNWTALGIPGVGHPVESMVDPWGLLTPVPGGPSLDWWVAVEGGALG
                     TMAPSQFDVVQRLQGDLPVVVTAYEGDGLRVSSEAWLLPLPDGDWAAMQVVLFNIADL
                     PLRGVFQFAMRPYNPEGVSPIYNIACDGGTLFTGGRPGPITWPA"
BASE COUNT          293 a          657 c          619 g          350 t
ORIGIN      
        1 tgctcgcggg gccgtaatgc acgatgatgc tgggggacct gggggccgaa gtgataaagg
       61 tagagtcgcc ggagggtgat gacacccgcc gctgggggcc tccctaccag ggcagcgagt
      121 cggcttacta cctttgctgc aaccgcaaca agcgcggtat cgtgctcgac ctgggcagac
      181 ctgtagaccg cgaaacggcc cgcgagcttg gcatacgcag ccatgttgtt gtggagaact
      241 ttcgcctggg gacgatggaa aagtgggggt tgagctacga ggcgctgtct gccgagaatc
      301 ccggccttgt ctactgctcc atctcaggct atgggcgcac cggccccgac gcccacctgc
      361 ccggctacga ttacgtggtg cagggtgccg ggggcatcat gagcgtcacg ggagagccgg
      421 acgcccctcc cgcgaaggta ggggtcgcca tcgtggacct gacggcgggc atgttcgctt
      481 gctctgcaat actggcagcc ctcagggtgc gcgacctcac cggcaggggc cagcgcattg
      541 acatctccct gctcgactcc cacctggcgt ggctcgccaa cgtcggcagc aattacctca
      601 tttcgggtga ggtgcctgcc cgctacggca acggacatgc caatatcgtg ccttaccaga
      661 cctttgccac ctccgatggc tgggtggtgc tcgcgaccgg caacgaccgg caatggcagc
      721 gattctgcat ggctatcgag cgccccgacc tcctggccga tcctcggttt gccacaaatc
      781 atgctcgcgt cgagcatcgc ggcatgctcg ttcctatgtt ggaggccatc ttccttacgc
      841 gcaccaccca tgagtgggtt gccctgtgcg ccggggcgga cgtgcccgca ggccccgtca
      901 atacggtgga ccgcgctctc aacagccctc aggccctggc ccgcgacatg gtgcggagcg
      961 tggaccaccc tggcatcggc cccgtgcgta tggtggcctc gcccctgaag ctggagagca
     1021 ctccgcccac tatacgccgt cacccgccta tgcttggcga gcacacggag gaagtgctgc
     1081 gtgaagtgct gggcgcgagc gtggcgcaat cccccgcgag cggaggcggc gagtgagacg
     1141 ccatcctcgg cttgacgcgt ggatgctggg gttgaagcgt gtcagatcac ccaggcgcgc
     1201 taactcccgc atccgccata tgcgccccca ggtggcgcgg gctgtgcgcc tgctgcgcgc
     1261 ggcatcccgc ctgggtgtgc ccggctctgt gcccgcgccg cccgaactgc tacctgcggg
     1321 cgcgcgggcg cttgcccagg gctacctcaa caggttctct atgcccttgc tgaacggctg
     1381 gctcctcccg ccctggatgc ggcggcagtc ggaccccgcc tcgcccttgt tcaccccgcg
     1441 cagcgtctcc aacctcatgc tgaaccagac cgcccgcaac tggacggctc tcggcatacc
     1501 tggggtgggc caccctgtag agagcatggt tgacccctgg ggcctcctca cccccgtgcc
     1561 cggcggcccc tcgctcgatt ggtgggtggc tgtggaaggc ggcgctcttg gtactatggc
     1621 cccttcgcag ttcgacgttg tgcagcgctt gcaaggggac ctgccggtgg tcgtgaccgc
     1681 ctacgagggg gatgggctgc gcgtatcaag cgaagcctgg ctgctgcccc tgcccgacgg
     1741 cgattgggcc gccatgcagg tcgtcctctt caatatagcc gacctgcctc tgcgcggcgt
     1801 cttccagttc gcaatgcgcc cctacaatcc ggagggcgta tctcctatct acaacatagc
     1861 ctgcgatggc ggtacgttat tcaccggagg ccgccccggc cccataacct ggcccgcgc
//
LOCUS       DASRWZ010000138         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_765792, whole genome shotgun sequence.
ACCESSION   DASRWZ010000138 DASRWZ010000000
VERSION     DASRWZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765792"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VFH60_02040"
     CDS             <1..777
                     /locus_tag="VFH60_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6312589.1"
                     /translation="RPQGVHVEFVRSPDRVTVPLELFDAAIDENTALVATSHVYFTSG
                     AIQDIKTLARMAHERGAMLLVDAYQATGHIPTDINELGADFYLSGSLKWLLGGPGITF
                     LYASEPACLQEPTVAGWWGMGNMFDFDVTSLQFRSEATRYELGTPSMAAVYAALGGCS
                     IVNEIGSERIRERDIALTEDLIRRALEAGFKPRVAPTPEQRTPIVLLDFDDPKPIVAA
                     LARQKIVVDFRPGAVRVSPYFYNTIEENEAVIQAIKAVAR"
     gene            855..2165
                     /locus_tag="VFH60_02045"
     CDS             855..2165
                     /locus_tag="VFH60_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312590.1"
                     /translation="MTNTPEELARSAPQRIASAIPKGVAGPLQRVRALAEERGWSTYV
                     VGGFVRDALLGIPQKDVDLTVVGDGVALAHLLASELGTEAEVADRFGTATVDIGGGLD
                     LDFVTARKESYPSPGALPEVAAGSLDDDLARRDFTINALAVPLEGAGFGKLIDGHGGT
                     ADIASGLVRVLHPRSFEDDPTRIFRAVKYAERLGFKLERGTLELILQAVRDGALGTIS
                     TERAVRELLAIMEEPRAEAMLAALDRLGVLPSVHPGLAWRYPPGRVQLRDDSKLTPGQ
                     RRDAYLATIAAEYARAPEEAEALARWLRLPAPLVRLMRDAAVLAGLWPRLSNEELKPS
                     EVYGMLNGLSDAALEAFGRLEVLRADSVGWGRFQEYLTLTRNVKPQLTGDYLRTLGVK
                     EGPVYREALRALHDAVLDGPVRGKEGEEKFVREWLRERGLLGER"
     gene            2829..>3478
                     /locus_tag="VFH60_02050"
     CDS             2829..>3478
                     /locus_tag="VFH60_02050"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET6312591.1"
                     /translation="MERATASPLPNRSFFGMNLYVTGLERSKLERTAVLNAATDLGVK
                     WSREEMSWANLEPYEKGEYNWKAYDPWIDELVKRDIDVIGAIQTSPSWASGVPQDAGP
                     DWYWYVPRNPQDFVDFAGVAAAHYKGKIDVWEIWNEQDVEMTFKCNGCDRVKVYAEML
                     AGSYAAIKQANPEATVLVGGLSIHDRTNAGLAFLEQVVAASGGKLNFDVLSIHPYMP"
BASE COUNT          631 a         1087 c         1132 g          628 t
ORIGIN      
        1 cggccccagg gcgtgcacgt cgagttcgtg cgcagcccgg accgcgtgac ggtgcccctc
       61 gaactgttcg atgccgccat tgacgagaac accgccctgg tcgccacttc gcacgtatat
      121 ttcaccagcg gggcgataca ggacatcaag accctggcgc gcatggccca cgagcgaggc
      181 gcgatgctgc tggtggatgc ttaccaggcc accggccaca tccccaccga catcaacgag
      241 ctgggtgcgg acttctacct gtcgggcagc ctcaagtggc tgctgggcgg ccccggcatt
      301 accttcctct acgcaagcga gcctgcctgc ctgcaagagc cgacggtggc gggctggtgg
      361 ggcatgggca acatgttcga cttcgacgta acgtcgctcc agttccggtc cgaggcgacc
      421 cgctacgagt tgggcacgcc cagcatggcc gccgtgtacg ccgcgctggg gggttgctcc
      481 atcgtgaacg agatcggctc cgagcgtata cgcgagcggg atatagccct caccgaagac
      541 ctcatccgcc gcgcccttga agcaggcttc aagccacgcg tggcccccac gcccgaacag
      601 cggacgccga tagtgctgct cgacttcgat gacccgaagc ccattgtggc agccctcgcc
      661 cgccagaaga tcgtcgtgga cttccggccc ggcgccgtgc gcgtctcgcc ctacttctac
      721 aacacgattg aggagaacga ggccgtgata caggccatca aggctgtagc gcgctaggac
      781 gaaagcgcct catgtggcgg cacaggcatc gacaggcata cctcccggcc acgtgcgccc
      841 ggcacctctg ctccatgacc aacaccccag aagaactagc caggtcggcc ccgcagcgca
      901 tagcgagtgc gattcccaaa ggcgtggcag ggccgctaca gcgggtgcgc gcgctcgcgg
      961 aggaacgcgg gtggtcgacc tacgtcgtgg gggggttcgt gcgcgacgct ttgttgggca
     1021 tcccccagaa ggacgttgac ctcaccgttg tgggagacgg cgtggccctg gcccacttgc
     1081 ttgcgtcgga gcttggcacc gaggccgagg tggcggaccg cttcggcacg gctacggtgg
     1141 atatcggggg agggctggac ctcgattttg tcaccgcccg caaggagagc tacccgtcgc
     1201 ccggtgcctt gcctgaagtg gcggcgggta gcctggacga tgacctcgcc cgccgcgact
     1261 tcacgatcaa cgccctggcc gtgccgcttg aaggggctgg gttcggcaag ctcatagatg
     1321 ggcatggcgg gacggcggat attgcgtcag gattggtcag ggtgctacac cccaggagct
     1381 tcgaggacga cccgacccgc atcttccgcg ccgtgaagta cgccgagagg ctgggcttca
     1441 agctcgagcg cggtaccctg gaactgatac tacaggcggt gcgcgatggc gcgctgggca
     1501 caatttcgac cgagcgggcc gtcagggaat tgctcgcgat tatggaggag ccgcgtgcgg
     1561 aagcgatgct ggcggcgctc gacagattgg gcgtgctgcc gtcggtgcac cccgggctgg
     1621 catggcgcta tccaccgggg cgggtccaac tcagggacga tagcaagctc acgccgggcc
     1681 agaggaggga cgcgtacctg gcgacaatag cggccgaata cgcacgcgcg ccggaggaag
     1741 cagaggccct cgctcgctgg ctgcgactgc ccgcgcccct cgtgcgcctg atgcgagatg
     1801 ccgctgtgct cgcgggtctc tggccccgcc tcagcaacga agaattgaag ccatccgaag
     1861 tgtacggcat gctgaacggc ctgagtgatg ctgcgttgga agcgtttggc cggttggagg
     1921 tattgagggc cgatagcgtg ggctgggggc ggttccagga gtacctgacg ctcacgcgaa
     1981 acgttaagcc gcagctaacc ggcgactact tgcggacgct gggggtgaaa gaaggtcccg
     2041 tgtacaggga ggcgctacgg gcgctgcacg acgcggtgct ggacggacct gtacgtggga
     2101 aagagggcga ggaaaagttc gtgcgcgagt ggctgcgcga gaggggcctg ttaggggagc
     2161 gatagcggga ggggcgacgc accggcaacc acataaccgt gcctggggcc gccccttcaa
     2221 aaggaccgga agactcgaac aaatgcaagc tacttcttgg cagtcttctc agacttggta
     2281 gcgacttgtt tcttcttctt ctgattgttc ttgggactct tgtcgcccat gtgccaattc
     2341 cccctttcgt agagtcggga gggaaccccg tggaaaactt atatcatgcc aggaggctgc
     2401 tgtcaatggt ccgtagatgt aggtgtgagg gcatttattc tcagaaggga ctagtgtgcc
     2461 ctgcaaccgc gccctgccaa tgaattggcg ggcttaccag ggacgaagtc tcctacggag
     2521 actacatgat gttgcttagt ccccgcagga gactttgcct cttgtaagcc cgccacttta
     2581 gtggcagggc gcggttgcag ggcatccccg catggctacc gctaggctag agactacacc
     2641 tctcccgcca cccggcgcgt ttgacccagt gcccgccctt ccttataatt gcgctacgtg
     2701 cccgttcccc tactctacta gaactgagtc ccctcttgag caaacatcgc ttcgcttcca
     2761 ggttgcgcct ttttctttct ctagcacttc tcctgtcgat gctaccccgg atgggtgctt
     2821 cgcctgttgt ggaacgggcg accgcctcgc cgctccccaa ccgctctttc ttcggcatga
     2881 acctgtatgt gacgggcctg gagcgcagca agctggagag gaccgccgtg ctgaacgccg
     2941 ccaccgacct gggggtcaag tggagccgcg aggagatgtc ctgggccaat cttgagcctt
     3001 acgagaaggg cgaatacaac tggaaggctt acgacccctg gattgacgag ctggtcaagc
     3061 gcgatatcga cgtgattggc gctatccaga cctccccctc ctgggcctcc ggcgtgccac
     3121 aggacgcggg ccctgactgg tactggtacg tcccgcgcaa ccctcaggac ttcgttgact
     3181 tcgcgggcgt ggcggcggcc cactacaagg gcaagatcga cgtctgggaa atctggaacg
     3241 agcaagacgt ggagatgacc ttcaagtgca acggctgcga ccgcgtgaag gtctacgccg
     3301 agatgctggc gggcagctac gcggctatca agcaggccaa cccggaggcc accgtgctgg
     3361 tcggcggtct ctccatccac gaccgcacca acgcgggcct ggccttcctg gaacaggtgg
     3421 ttgcggcctc aggcggcaag ctcaacttcg acgtgctctc catccacccc tatatgcc
//
LOCUS       DASRWZ010000139         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_355069, whole genome shotgun sequence.
ACCESSION   DASRWZ010000139 DASRWZ010000000
VERSION     DASRWZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355069"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="VFH60_02055"
     CDS             <1..1899
                     /locus_tag="VFH60_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6312592.1"
                     /translation="NAGIALFRTSNPANFVFANRLDAVGSISEGDPLYKEGDGYPGLT
                     PFSIDYSFYRDLRGSNGPKDTNDNAADFLFVDTQGTPAGAGQRLGAPGPENLSSPTYG
                     NLPGGSGPEGSPPVSISLLDPGVPANAAPNFVRDTTSDPDNNSWSGTITVRRKITNNT
                     GVNITRLRFRITDITTFPEPSAITTTADLRARTSNAVVVSTSGAGNITVQGTTLEEAT
                     GDMVGQPHGGAYNSTLSVGSITLATPLPPGQSVNVQFLFGVQQAGCFRLAFDMPGLDY
                     TGTTDSAVCSAAPPTATSTSTATRTATNVPASTNTTTTTHTATRTATNVPASTNTATR
                     TATRTATSTVTNTPGAPGSSPTSTTQVPPTSTSMPPTATRTATQQAATATATATQPGP
                     GPPTATRTSQAVATATSVPSTCSLQFGDVPAGSAFYSYVRCLTCQTVMGGYPCGGANE
                     PCTDPGATYFRPSALITRGQIAKIVSNAAGFEDTVPTGQQTFEDVAPGSAFWAYIERL
                     TSRGIMSGYNCGGTAEPCGQGNRSYFRPGANATRGQLAKIVANAAGFSDTHSNQMFTD
                     VPASAPFFTWIERLAARGYISGYPCGGPGEPCDAANTPYFRANDNVTRGQASKIVSNT
                     FYPNCQTP"
BASE COUNT          403 a          778 c          565 g          333 t
ORIGIN      
        1 aatgccggta tcgccttgtt ccgcacctcg aaccccgcca actttgtgtt cgcgaaccgg
       61 ctcgacgcgg taggctccat cagcgagggg gaccccctct acaaggaagg cgacggctac
      121 cctgggctta ccccgttctc gatcgattac tccttctacc gcgacctgcg aggctccaac
      181 ggccccaaag acaccaacga caacgctgcg gacttccttt tcgtagacac ccagggcacc
      241 ccggcgggcg cgggccagcg tctcggcgct cccggccccg aaaacctcag cagcccgacc
      301 tatggcaacc tccccggcgg ttccggcccg gagggcagcc ccccggtctc gatcagcctg
      361 ctggaccccg gcgtgcccgc gaatgccgca cccaacttcg tgcgcgacac cacgtccgac
      421 ccggataaca actcctggtc cggcacaata acggtccgcc gcaagataac caacaacacc
      481 ggggtcaaca tcaccaggct gcgcttccgc atcaccgaca tcacgacctt ccctgagccg
      541 agcgcaatca ccactacggc agacctgcgg gcgcgcacct ctaacgccgt cgtggtgagc
      601 acatccgggg ccggtaacat caccgtgcag ggcactaccc tggaggaggc gaccggggat
      661 atggttgggc agccccatgg cggggcttac aattctacgt tgtccgtggg cagcataacc
      721 cttgccactc cactcccgcc tggccagagc gtcaatgtgc agttcctgtt cggcgtgcag
      781 caagcgggtt gcttccggct cgcgttcgac atgcccggcc tcgactacac gggcaccacc
      841 gatagcgctg tgtgctcggc ggctcccccc accgctacgt ctacatctac ggccacccgt
      901 actgccacca acgtgcctgc cagcaccaat accactacca caacgcacac tgcgaccagg
      961 acggctacca acgtgcccgc cagcactaac acggctaccc gcacggccac caggactgct
     1021 accagcaccg tcaccaacac tcctggggct cccggttcca gcccgacctc taccacccag
     1081 gtgccgccga ccagtacctc aatgccgcct accgccacac gcaccgctac ccagcaggcc
     1141 gccacggcta cggccacggc tacccagccc ggtccggggc cgccgacggc cacccgcacc
     1201 tcccaggctg tggcaaccgc taccagcgtg ccctccactt gcagcctcca gttcggtgac
     1261 gtgcccgccg ggagcgcctt ctacagctac gtgcggtgcc tcacctgcca gaccgtgatg
     1321 ggcggctacc cctgtggcgg ggccaacgag ccttgcacag accctggcgc gacttacttc
     1381 aggccctccg cgctaatcac cagggggcag atagccaaga tcgtgtccaa cgcagcgggc
     1441 tttgaggaca ccgtgcccac aggccagcag accttcgagg acgtagcccc cggtagcgcg
     1501 ttctgggcct acatcgagcg cctgacgagc cgaggcatca tgagcgggta caactgcggc
     1561 ggcactgccg agccttgcgg gcagggtaat cgctcgtact tcaggcccgg cgctaatgcc
     1621 acgcgcggcc agctcgccaa gatcgtggcg aacgccgccg gcttcagcga tacgcactcc
     1681 aaccagatgt tcaccgacgt gcccgcctcg gccccgttct tcacctggat agagcgcctg
     1741 gcagcccgtg gctacatcag cggttacccc tgtggcggcc ccggcgagcc gtgcgacgct
     1801 gctaacacgc cctacttccg cgccaacgac aacgtcacgc gaggccaggc ctcaaagatc
     1861 gtatccaaca ccttctaccc caactgccag actccctagg gcttcttggg agagggaaac
     1921 gggagtacga aaacgcaaag acgcaaagac gcaaagaacg cgcagagatt aggattatgg
     1981 tgttctaagc aagaggacca gggtacatgt catcgtaccc tggtcctctt gtactcaccg
     2041 aatctttatt ctctttgcgg gttctttgcg tctttgcgt
//
LOCUS       DASRWZ010000140         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_491676, whole genome shotgun sequence.
ACCESSION   DASRWZ010000140 DASRWZ010000000
VERSION     DASRWZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491676"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1187)
                     /locus_tag="VFH60_02060"
     CDS             complement(156..1187)
                     /locus_tag="VFH60_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET6312593.1"
                     /translation="MRLKGTNYWRSDRPGVNTWAEWDGPQVWQELLKARELGINTIRI
                     GLPYDTPMGMDEVWGEGCEGRPDRCSEVRGPLVNHMTQFLQLASDNGMKVLFVLFDWS
                     DSFPEPGTAEYRRQVNYLRAIVGPFADDDRVLGWDLHNEPENYNNWRLQGPARVVAWV
                     EDMAREVRAIDGRHPLTVGVASYQTMWLDAGGKQLLDMVDFVGFHSYEAGGLSGQIAE
                     TKKRTGKPILIGEMGWPTGPGRLSTPQKRYDEATQQFLYQAMIRDVAANDIAGMVQWT
                     LWDHPLGNPNHYIKPTIEAWFGLVRLDGTFKPAAGDFRDSYAVPLLPSTTKTDVPLTL
                     PGGAIAPKP"
     gene            complement(1200..>2195)
                     /locus_tag="VFH60_02065"
     CDS             complement(1200..>2195)
                     /locus_tag="VFH60_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312594.1"
                     /translation="TPMHHRRSVGLWLMLALHLPLVAAEFPISVHGAPSFAMSQFEQV
                     WSRTDRPVAQGAVSRSWLWGPQPGEVRTEPFVSAPGGTRTVQYFDKARMEANPAVTDP
                     SDFWSTTTGLLVVELVSGRVQVGPDSYETRTPADLPVAGDAGQSGDDTPLYSSFRELA
                     SLPGGADRRVPSSIGSPVLATIGRAGVVGALPGSEVRYTAYSQETGHNIPSVFERFMQ
                     ARGLVSEAGTLQEGALFDPVYLLGYPITEAYWATVAIDGKPTRVLVQLYQRRVLTYIP
                     SFSPEWQVQMGNVGQHYYTWRYTVPAAPTPTPAPTAVPRPGANDTFLHIQGSKFY"
BASE COUNT          361 a          699 c          730 g          405 t
ORIGIN      
        1 aagataacta aggtattcta agtaaaagga ccagagtacg acatgacgta ccctggtcct
       61 ttttgtactc tccaaaccct ttcttctctt tgcgcgtcct ttgcgtcctt tgcgtctttg
      121 cgttttcgtc tccaactgct actatcgccc ctgtgctagg gtttcggagc aattgcgccg
      181 cccggcaacg taaggggcac atcggttttg gtggtgctgg gtaggagggg gacggcgtag
      241 ctgtcgcgga agtcgcctgc cgcgggcttg aaggtgccat ccaggcgcac caggccgaac
      301 cacgcctcga tagtgggctt gatatagtgg ttggggttgc ctagggggtg gtcccagagg
      361 gtccactgca ccatgcccgc tatgtcgttg gcggccacgt cgcggatcat cgcctggtag
      421 aggaactgct gggtggcttc gtcgtagcgc ttttgcgggg tggagagcct gccaggcccg
      481 gtgggccagc ccatctcgcc gatcagtatc ggcttgcccg tgcgcttctt ggtctcggct
      541 atctgcccgc tgaggccccc cgcctcgtag ctgtggaagc caacgaagtc caccatgtcg
      601 agcagttgct tgccgcccgc gtcgagccac atcgtctggt agcttgccac gcccaccgtg
      661 agcgggtgcc tgccgtcgat agcccgcacc tcccgcgcca tgtcctccac ccaggcgacc
      721 acgcgggccg ggccttgcag cctccagttg ttgtaattct caggctcgtt gtgcaggtcc
      781 cagcctagca cacggtcgtc gtcagcgaag gggccgacga tggcgcggag gtagttcacc
      841 tgccgccggt actccgccgt gcccggttcg gggaagctgt cgctccaatc gaacagcacg
      901 aagagcactt tcataccgtt gtcggaggcg agttgcagga actgggtcat gtgattgaca
      961 agtgggcctc gcacctcgga gcacctgtcg gggcggcctt cgcagccctc gccccacact
     1021 tcgtccatgc ccataggggt atcgtagggc aggccgatgc gaatcgtgtt gatgcccagc
     1081 tcgcgcgctt tgagtagctc ctgccacacc tgggggccgt cccactcggc ccacgtgttc
     1141 acgccgggcc tgtcgctgcg ccagtaattg gtgcccttga ggcgcacagg ctcgccacgt
     1201 tagtaaaatt tgctcccctg gatgtgcagg aaggtgtcgt tagcgccggg gcgcggcacg
     1261 gcggtagggg cgggagtggg cgtgggcgcg gcggggactg tgtagcgcca ggtgtagtag
     1321 tgctggccca cgttgcccat ttgcacctgc cactccgggc tgaagctggg tatgtaggtc
     1381 agcacgcggc gctggtaaag ctgcacgagc acccgcgtgg gcttgccgtc aatcgccacc
     1441 gtggcccagt acgcctcggt tatggggtag ccgagcaggt acacagggtc gaacagcgcg
     1501 ccctcctgca aggtgcctgc ctcgctcaca aggccgcgcg cctgcatgaa ccgctcgaac
     1561 acggagggga tgttgtggcc ggtctcctgg gaataggcgg tgtagcgcac ctcgctgccc
     1621 ggcaaagccc ctaccactcc cgccctgccg atcgtggcga gcacgggaga accgatagat
     1681 gagggcacgc ggcggtcggc cccacccggc agggaagcca gttcgcgaaa cgagctatag
     1741 aggggcgtgt cgtcgccgct ctgccccgca tcacccgcca cgggcaggtc ggcgggcgtg
     1801 cgcgtctcgt agctgtccgg ccccacctgc acgcggccgc tcacaagctc caccacgagc
     1861 aagcccgtgg tggtggacca gaagtcggaa gggtccgtca cggcggggtt cgcctccatg
     1921 cgggccttgt cgaagtactg cacggtgcgg gtgccgccgg gggcagagac gaaaggctcg
     1981 gtgcgcacct cccccggctg cggcccccac agccacgagc gcgacacggc accctgcgcc
     2041 acgggccggt cggtgcgcga ccacacctgc tcgaattgcg acatggcgaa cgaaggcgcg
     2101 ccatgtacgg agatagggaa ctctgcggct actaacggca aatgcaaggc aagcatgagc
     2161 cagaggccca cagaacggcg atggtgcata ggggt
//
LOCUS       DASRWZ010000141         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1039923, whole genome shotgun sequence.
ACCESSION   DASRWZ010000141 DASRWZ010000000
VERSION     DASRWZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039923"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFH60_02070"
     CDS             <1..270
                     /locus_tag="VFH60_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6312595.1"
                     /translation="ITWVPLSHDWEPHSPYPDMAASHAESDAIQRALSTVPADMRVCL
                     VLYMYQGLAYKEIADTLGISVNLVAVRIYRGREKFIEAYKKGEGG"
     gene            359..2629
                     /locus_tag="VFH60_02075"
     CDS             359..2629
                     /locus_tag="VFH60_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6312596.1"
                     /translation="MRHTCQDVQLLISKYVDAEATPEEREIVDLHVVACLECARKMTE
                     YMEIAAIFAEAPMRAPQPDLRAGLFREIGNIKDEARRKEDLAARRRKWYRWSPPAARP
                     AASTQLVPRGRFRWGRLWDLASPFAAASMAMLAIVAVVLTYGNKARDNASDVPAIANY
                     PDFPAVSTIPATVVYPMMVSTEGGAKVYEQVPPIKTGLAGPAPLAATTVPVQGTPGIS
                     EDVALQLEQATPVLEADDAWHQLRDPAYGYVVSYPPNWWTQSRGNTRYFYPWTEGGTQ
                     HAPYRIELHVDANTEGLDAAAANQALLGGKGRLEGREGGAVWLRSSGGDDASVTDAIY
                     AFDPNFIYTLRLTVPKESPLGDFESRWGRAQDLFSRMSRNVSLGGQQGRVLFLNGGDL
                     WSVSATGPGNPMALWKGYNTRWTRQFALSPDGHTVAFGTTNATLDPWANELNLAQLDG
                     TGSNQAQPILSGAEIHDIAWYADRELLAIADLADSGLGIYRLTLDADHKAGPPELLVA
                     LGEDLMGARGLTVSPDRQLISFMAPLGESKSTDIYAVRPDGGNLLKLVTHADALSPSQ
                     PGGQGSPAEQQAIKSYVWADGSLEPGQGGYRYGLLYTRGNSASPTLYRGGLLYGAPNP
                     RNGPLLDPASLDKPDAASVQIVHVAYSPAGKVAMSGFYSLRGLKVESLAGLWTADFTG
                     GTLTNVRPLPTPDASHGVADLQWSPDGTAIIYREMVPSSEADFASRYDGRSPFSIVKL
                     DITTGQRTVLYSAPGR"
BASE COUNT          550 a          962 c          844 g          434 t
ORIGIN      
        1 atcacctggg tgcccctgtc gcatgattgg gagccgcatt ccccgtaccc cgacatggct
       61 gccagccacg ccgagagcga cgcgatacaa cgcgccctca gcacggtgcc cgccgacatg
      121 cgcgtctgcc tggtgctcta catgtaccag ggcctcgcgt acaaggaaat cgccgacacg
      181 ctaggcatat cggtcaatct ggtggccgtc cgcatctatc gcggccgcga gaagttcatc
      241 gaggcgtaca aaaagggaga agggggatag cattttagat tttagatttt ggattttgga
      301 tttgaaggac ttcctaacaa tccaaaatcc aaaatctgaa atctaaattc cacaactgat
      361 gcgccacact tgccaggacg tacagctact catatcgaag tacgttgacg cggaggccac
      421 gcccgaagag cgggagattg tggacctcca tgtcgtggca tgcctggagt gcgcccgcaa
      481 gatgaccgag tacatggaga tcgcggctat attcgccgag gcccccatgc gcgcccccca
      541 gcccgacctg cgcgcgggcc tgttccgcga gataggcaac atcaaggacg aggccaggcg
      601 caaagaggac ctggccgcgc gacgccgcaa gtggtacagg tggtcgcccc cagccgctcg
      661 ccccgccgcc tcaactcagc ttgtgccgcg cggacggttc aggtggggga ggctgtggga
      721 cctggctagc cccttcgctg cggccagcat ggcgatgtta gccattgtcg cggtggtgct
      781 cacgtacggc aacaaggcca gagacaacgc ctctgacgta ccggccatcg ccaactaccc
      841 cgacttcccc gccgtctcca ccatacccgc caccgtggtc taccctatga tggtaagcac
      901 ggagggcggc gctaaagtct acgagcaggt gccccccatc aagacggggc tggcaggccc
      961 ggcccccctg gcggccacaa ccgtgcctgt gcaaggcaca cccggcatca gcgaggacgt
     1021 ggcgctgcaa ctcgaacagg ccaccccggt cctggaagcg gacgacgcct ggcaccagct
     1081 acgcgacccc gcatacggct acgtcgtctc gtacccgcct aactggtgga cccagtcgcg
     1141 gggcaacact cgctacttct acccctggac ggagggcggc acccagcacg ccccataccg
     1201 catagagctg cacgtggacg ccaataccga gggcctcgac gccgccgccg ccaaccaggc
     1261 gctgctcggc ggcaagggcc gtctggaagg ccgggaggga ggcgcggtct ggttgaggag
     1321 cagtgggggc gacgatgcca gcgtcaccga cgcgatctac gccttcgacc ccaacttcat
     1381 ctacacgcta cgcctcaccg tgcccaagga aagcccgctg ggcgacttcg agtcgcgctg
     1441 gggccgggca caagacctgt tcagccggat gagccgcaac gtgtccctcg gtgggcagca
     1501 gggccgggtg ctcttcctca acggcggcga cctctggtcg gtgagcgcca cgggaccggg
     1561 caacccgatg gccctgtgga agggctacaa cacccgctgg acccgccagt tcgccctctc
     1621 ccccgacggc cacacggtag ccttcgggac tacgaacgcc accctcgacc cgtgggccaa
     1681 cgagctaaac ctcgcgcaac tggatggcac cggctccaac caggcgcaac ccatcctctc
     1741 cggcgcggag atacacgaca tcgcctggta tgccgaccgc gagctactgg ctatcgcgga
     1801 cctggcggac tccgggcttg gcatctaccg gctcacgctt gacgcagacc acaaagcggg
     1861 ccctcctgag ctactggtgg cgcttgggga agacctgatg ggggccaggg gcctcaccgt
     1921 gtcacccgac aggcaactca tctcgttcat ggccccgctc ggcgagagca agagcaccga
     1981 catttacgcc gtgcgtccag acgggggcaa cctcctcaag ctggtgacgc atgccgatgc
     2041 gctgtcgcct tcgcagcccg gcggccaggg tagccccgct gagcagcagg cgatcaagtc
     2101 ctacgtgtgg gccgacggca gcctggaacc ggggcagggc ggctacaggt atggcctcct
     2161 ctacacacgc ggcaactcgg ccagccccac tctttacagg ggcggcctcc tctacggcgc
     2221 gcccaacccg cgcaacggcc ccttgctaga ccccgcctcg ctcgacaagc ccgatgcggc
     2281 ctcggtgcag atcgtccacg tggcctattc gcccgcaggc aaggtggcga tgtccggctt
     2341 ctacagcttg cggggcctca aagtagagtc gctggcgggg ctgtggacgg ccgacttcac
     2401 gggcgggacg cttaccaacg tgcggcccct acccacccct gacgcttctc acggcgtagc
     2461 cgacttgcag tggtctcccg acggcaccgc gatcatctac cgagagatgg tgccctcaag
     2521 cgaggccgac ttcgcctccc gctacgacgg ccgctccccc ttctccatag tcaagctcga
     2581 cataacaacc ggccaaagga ccgtcctcta cagcgcgccg gggcggtaag cggcactgga
     2641 ttacgaaaac gcaaagcatg ccctgagcgc agccgaaggg acgcaaagga cgcaaagaac
     2701 gcgcagagat taagttaaag gtgtcctaag caagaggacc agggtacgtc atgacgtacc
     2761 ctggtcctct tgcttaggac acctttaact
//
LOCUS       DASRWZ010000142         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1039962, whole genome shotgun sequence.
ACCESSION   DASRWZ010000142 DASRWZ010000000
VERSION     DASRWZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039962"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..2095
                     /gene="pepF"
                     /locus_tag="VFH60_02080"
     CDS             293..2095
                     /gene="pepF"
                     /locus_tag="VFH60_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET6312597.1"
                     /translation="MAIALPKRGELDAKYTWDLGSIFTDNEKWEAKAGEVRAMLPELD
                     GYKGHLGESAQQLLGYFKLAERINVPMMQLFVYANMMHDSDTTNQETAALRDQARGMF
                     ASVAAALSFAEPELLTVPQEKIDRFMEEEPELQVYRHYFDMLRRRAEHVRSSEVEQVL
                     AMASDVLDTPRSAHGTLADSDLDYGSVDTGESELKVTGGTIQSLLRNPDIEVRRKAWE
                     QHADGFLSVKNTFATLLAGGVKKDVFLARAHNYGTAVEAALAQNFIPREVYDNMLNTA
                     RRKLPVWHRYWSVRRRILGLDMLHPYDVFAPLSKEQPRLAFEEAVDMICEGMGPLGGD
                     YCDPMRKGLLEQRWVDVYPNVGKRSGAYSGGSYGTNPFILMSYANDLESMSTLAHELG
                     HSMHSYLTRANQPMVYARYGMFVAETASNFNQALVRAHLLKKNDDPQFQISVIEEAMY
                     NFHRYFFIMPILARFELEIHTRVENGQALTADGLIELMADLFAEGYGPEVEVDRPRVG
                     ITWAQFPIHMYLNFYVYQYATGISAANALAANVLEKGESAAEDYKRFLKAGSSTYPLE
                     VLKMAGVDMSTPEPVERAFDILEGFIDRLERLAL"
BASE COUNT          465 a          688 c          705 g          401 t
ORIGIN      
        1 aaggtggcgc gctccccggc atgcctacca ccggccagtc tgacacgcct atgctactct
       61 cgctgctgct ggcggcgttg ggtctgctag gcatcggcgt cctggcacgt cgcgcaaaga
      121 ccaccggacg ctaacagtac ggaatttgga aattagaaat gcggatttcg gatttgaagg
      181 agcgcttcct caaatccgaa atccgcattt ctaattagcc cttttccttg acattcaggc
      241 ccggtattgt agatatttat ctatactatt cagtgagagg agcgatatcg atatggcaat
      301 agctttaccc aagcgtggcg agcttgatgc gaaatataca tgggacctgg ggagcatatt
      361 caccgataac gagaagtggg aggcgaaagc gggggaggtc cgcgccatgc tgccggagct
      421 tgacgggtac aaggggcacc tgggcgagtc ggcccagcag ttgctcggct acttcaagct
      481 ggcggagcgc atcaatgtgc ctatgatgca actgttcgtg tacgccaaca tgatgcacga
      541 cagcgatacc accaaccagg agaccgccgc cctgcgcgac caggcgcgcg gcatgttcgc
      601 ctccgtcgcg gccgcgttgt ccttcgcgga gccggaactg ctcaccgtgc cgcaggagaa
      661 gatcgacagg ttcatggagg aggagccgga gcttcaggtc tacaggcact acttcgacat
      721 gctgcgccgc agggccgagc acgtgcgctc gtccgaggta gagcaggtgc tggctatggc
      781 ctccgacgtg ctcgacaccc cccggagcgc ccacggcacc ctggccgact cggacctcga
      841 ctatggcagc gtcgatacgg gcgagagcga attgaaggtg acgggcggaa ccatccagtc
      901 gcttttgcgc aaccccgata tcgaggtgcg ccgcaaagcc tgggagcagc acgccgacgg
      961 gttcctgtcg gtgaagaaca ccttcgcgac gttgctggcg ggcggtgtaa agaaggacgt
     1021 gttcctcgcc cgcgcccaca actacggcac ggcggtggag gcggccctgg cgcagaactt
     1081 catcccgcgc gaagtgtacg acaacatgct caacacggcc cggcgcaagc tccctgtgtg
     1141 gcaccgatac tggtcggtgc gccgccgcat cctgggcctc gacatgctcc acccttacga
     1201 cgtgttcgct cccctctcga aagagcagcc caggctggcg ttcgaggaag cggtggacat
     1261 gatctgcgag ggcatggggc cgctgggtgg cgactactgc gacccgatgc gcaaagggct
     1321 attggagcag cggtgggtgg acgtttaccc gaacgtgggc aagcggagcg gcgcgtactc
     1381 cggcggctcc tacggcacca accccttcat cctgatgagc tacgcgaacg acctggagtc
     1441 gatgagcacg ctggcccacg agcttgggca ctccatgcac tcctacctga cgcgggccaa
     1501 ccagccgatg gtgtacgctc gctacggcat gttcgtggcc gagacggctt ccaacttcaa
     1561 ccaggcgctg gtgcgcgccc acctgctcaa gaagaacgac gacccgcagt tccagatttc
     1621 ggtgatagaa gaggcgatgt acaacttcca ccgctacttc ttcatcatgc cgatactggc
     1681 ccgcttcgag cttgagatcc acacacgcgt ggagaacggg caggcgctca cggcggacgg
     1741 gctgatcgag ctgatggcgg acctgttcgc ggagggctac gggcctgagg tggaggtgga
     1801 caggccgcgc gtgggcatca cctgggcgca gttcccgatc cacatgtacc tcaacttcta
     1861 cgtctaccag tacgccacgg gcatctcggc cgccaacgcc ctggccgcga acgtgctgga
     1921 gaagggggaa agcgccgccg aggactacaa gcggttcctc aaggcgggaa gctccaccta
     1981 cccgctggaa gtcctcaaga tggcgggagt ggacatgtct acgcccgaac ccgtagagcg
     2041 cgccttcgac atcctggaag gcttcatcga ccgcctggag cgactcgccc tttagcggag
     2101 cgattttgat gaaaccgcaa aggcgcaaag agcgcaaagg taacactaag agcataaggc
     2161 ttcagagggt acgaaaggga ccagggcgcg acatcacgtg ccctggtccc ttcttgcact
     2221 gagagcaccg tgatatcgca ccctggtcct tcttgcact
//
LOCUS       DASRWZ010000143         1829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_286356, whole genome shotgun sequence.
ACCESSION   DASRWZ010000143 DASRWZ010000000
VERSION     DASRWZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286356"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..945)
                     /locus_tag="VFH60_02085"
     CDS             complement(352..945)
                     /locus_tag="VFH60_02085"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6312598.1"
                     /translation="MDSDSATRRNRWLDRQGVEGDAYDVQYIEREKAGENVHGEADFV
                     EAFRPTSVLDAGCGTGRVAIELARRGITAVGVDIDPRMLETARRKAPHLEWHQGDLST
                     INLGRKFDAVVMAGNVMIFVTSGTEGAVLKNLARHLVHGGVLVSGFQLGTGYLTIDEY
                     DRLAKEAGLELLERWSTWQRDPWDPRSTYALNVHRLG"
     gene            complement(984..1613)
                     /locus_tag="VFH60_02090"
     CDS             complement(984..1613)
                     /locus_tag="VFH60_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET6312599.1"
                     /translation="MAILLAGCGSAPPSAQPVDTTSSATTSLQAQGATPTATAWVPPT
                     ALAGTPIPGAVPTLAAAPPLERATLTLTNKAGEQFVMTVEIADTDEARGLGLMFRSSM
                     DPDAGMLFDFEEDTTSSFWMANTILPLSIAFIEANGNIIDIRDMQPLDTTSVGPGAPY
                     RYALETNQGYFRAHNLAPGDKVTLPGEQSAIIPGMPACPCVTNDCGAAQ"
BASE COUNT          321 a          533 c          603 g          372 t
ORIGIN      
        1 ggcattggta cttggatcga tagctcgggg cagacgccgg acgagacggt ggatgcggtc
       61 ctggctcgtc tttgacgtat gcttatgggt acataacccc gctcctctgt catttcgagc
      121 gcagcgagaa atctcgtcct ccagcaccca aagtccgcct tttctaccgg gaccacgctc
      181 ccgaaagaaa agtgcaagac cacggtgagg gacgagattt tctcgctgcg ctcgaaatga
      241 cgtaggtcgg ggctttggta ctgccagagc aggaggagca agagccaatg tcgttaggtc
      301 gggcatggcc cttgatgcgg gttgcttgtc cgtagcgacc ggcccctggt cttagcccaa
      361 cctgtgcacg ttcagcgcgt aagtgctacg agggtcccag gggtcgcgct gccaggttga
      421 ccagcgttcg aggagttcga ggcccgcttc tttcgcgagg cggtcgtact cgtctatagt
      481 gaggtagccg gtgccgagtt ggaagccgct gacgagcacg cccccgtgga ccaggtgccg
      541 ggccaggttc ttcagcaccg cgccctccgt gccgctcgtt acgaagatca tcacgttgcc
      601 tgccatgacg acagcatcga atttcctacc caggttgatt gtggagaggt cgccctggtg
      661 ccattcgagg tgaggggcct tgcggcgggc cgtctccagc atgcgggggt cgatatcgac
      721 acccacagcg gtaataccac gccgggccag ctcgatagct acccggcccg taccgcaacc
      781 cgcgtccagc accgatgtgg gccggaacgc ctccacgaaa tcggcctcac cgtgtacgtt
      841 ctcgcccgct ttctcgcgct cgatgtattg aacgtcgtag gcgtcgcctt ccacaccttg
      901 cctgtcgagc caccggttgc gccgggtagc gctgtcagag tccatgcgtg cccgtcctcg
      961 atgtgccggg tgtaacagac ccgctattga gccgcgccac agtcgttggt gacacatggg
     1021 caggcgggca tgccggggat gatcgcgctc tgctcgccgg gcaaggtcac tttgtcgccc
     1081 ggcgcgaggt tgtgcgcccg aaagtagccc tggttggtct ccagcgcgta gcggtagggc
     1141 gcgccggggc cgacgctggt ggtgtcgagg ggttgcatgt cgcggatgtc gatgatattg
     1201 ccgttcgcct cgataaaggc gatgctcaag ggcaggatgg tgttcgccat ccagaagctg
     1261 ctggtggtat cttcctcgaa gtcgaacagc atgcccgcgt cggggtccat gctgctgcgg
     1321 aacatcaggc caagcccccg cgcctcgtcg gtgtccgcta tctccaccgt catcacgaac
     1381 tgctcgcccg ccttgttggt cagggtaaga gtggcccttt cgaggggtgg ggcggcggca
     1441 agggtgggca cggcccccgg tatcggcgtg cccgcaagcg ctgtgggcgg cacccaggcc
     1501 gtagctgtag gcgtggcacc ctgcgcctgc aaggaagtcg tagcagagga ggtagtatct
     1561 accggctgcg cgctgggcgg agcgctccca caaccggcca gtagtatagc caagacaagg
     1621 gaaagcgctc caggcacgaa aagaggacgt ttctttctca tagtggatta tacaagtttg
     1681 gcgctgaaac gggaaaggtg gcgagacgag tggtcagtgg tcagtggtta gtggggcagc
     1741 ttatcgtagg tagttcgtga gtgggacagt tgctaatagt ggatcctgtc cccctttgtc
     1801 attctgagcg tagcgaagaa tctcgtccc
//
LOCUS       DASRWZ010000144         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_355234, whole genome shotgun sequence.
ACCESSION   DASRWZ010000144 DASRWZ010000000
VERSION     DASRWZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355234"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1056)
                     /locus_tag="VFH60_02095"
     CDS             complement(106..1056)
                     /locus_tag="VFH60_02095"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET6312600.1"
                     /translation="MSGRLQLFTGNAHPALAQAIAEHLGMKVGTAMVTQYRNGETRVV
                     IEENVRGNDVFIIQPTCHPVNHHLMEMLIMVDAIKRASAQRITAVMPYYGYAKQEKKS
                     RAREPITAKLVADLLQVAGVDRILTCDLHAPAIEGFFNIPVDHLQGRRLLADRCRELD
                     LPDPVVVTPDQGRIGWAMEVRERLGGHVDLAIIAKQRPTADRSYAVEMVGDVRGKTAL
                     LIDDMIMTGGTLCAAAEMLLDRGARAVYACATHAAFAPGTLELLETSSFTRVLISDTI
                     PVPGINRHETSKIEVVSIAQLFGEAILHIHEDRSVSALFR"
     gene            complement(1154..2611)
                     /gene="glmU"
                     /locus_tag="VFH60_02100"
     CDS             complement(1154..2611)
                     /gene="glmU"
                     /locus_tag="VFH60_02100"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET6312601.1"
                     /translation="MESQPLSVVVLAAGLGKRMQSRQPKVLHRLAGLPLAAHVLRALA
                     PLAPNHTVLVVGHGADQVEATLGNSYGLDGSLSITYANQPEQLGTGHAVLMAREALRS
                     YHGPVLVLYGDTPLIRTDTLNQLLSRHRQNRPHVTMLSAIVEDPTGYGRVMRDKDQNV
                     LAVVEERNASLTQRAINEINSGVYVFESDWLWNSLQGLQPNQPSGEFYLTDLIGMAIK
                     EERGTGTTSSLAMGARGKRRLLTFTLDGIEEAMGINSRAQLAEAEVIVQGRLRRQWLE
                     AGVTMLLPESIYLGMDVHLDPDTVLYPGVILEGTTRVATGCTLGPNTHIINSTIGENC
                     RIVASMIEGSTVERNVSVGPYSHLRPGAHLMEGVRMGNFGEVVRSTLEPNVNMNHFSY
                     VGDATIGEGTNIGAGTITANYDGVKKHRTTVGKNVFLGSDTMLRAPIEVGDGAITGLG
                     SVVTKDVQPHTMVVGVPARMIRRLEQTPQEAEEPQ"
BASE COUNT          477 a          884 c          917 g          580 t
ORIGIN      
        1 acttccttaa ctgctacgca acaatagtat accaattcgg ttccatactg cgtaaattac
       61 gaatcgcgag ttactcagtc ctccgtcctc agtcctcatc actcgctacc ggaagagcgc
      121 gctcaccgac ctgtcctcgt ggatgtggag gatggcctca ccgaatagct gggcgatgct
      181 tacaacctcg atcttgctcg tttcgtggcg gtttatgccc ggcacgggta tcgtatcgct
      241 gatgagcacc ctggtgaagg aggacgtttc cagcagttcc agcgtgccgg gggcaaaggc
      301 ggcgtgggtg gcgcaggcgt acacagcacg ggcaccccgg tccagcagca tctcggcggc
      361 ggcacagagg gtcccgcccg tcataatcat gtcgtcgatg agcagggccg tcttgccgcg
      421 cacgtcgccc accatctcca ccgcgtacga gcggtcggcg gtggggcgct gcttggcgat
      481 gatggcgagg tctacgtggc cgcccagccg ctcgcgcacc tccatcgccc aacctatgcg
      541 gccctggtcg ggggtcacca ccacagggtc gggcaggtca agctcccggc agcggtcggc
      601 cagcaggcgg cggccctgca agtggtccac cgggatgttg aagaagccct caatcgcagg
      661 ggcgtgcagg tcgcaggtga gaatacggtc caccccagcc acctgcaaca ggtcggccac
      721 cagcttggcc gtgataggct cgcgggcgcg gctcttcttc tcctgcttcg cgtagccgta
      781 atagggcatc actgccgtga tgcgctgcgc ggaggcccgc ttgatcgcgt ccaccatgat
      841 gagcatttcc atcaggtggt ggttgacggg gtggcaggtc ggctggatga tgaatacgtc
      901 gttgccgcgc acgttctcct cgatcaccac gcgggtctcg ccgttgcggt actgcgtgac
      961 catcgccgtc cccaccttca tgcccaggtg ttcggctatg gcctgggcca gcgcggggtg
     1021 cgcgttcccg gtgaatagct gcaatcgacc agacatatca gccctccacg cattctggaa
     1081 tttggatttt ggattttgga tctgctgcat cctagccctt ctcctgctct gtctgcgtct
     1141 cgtcggttgt gctttactgt ggctcttctg cttcttgcgg ggtctgctcc agcctgcgta
     1201 tcatgcgggc gggcacgccc accaccatcg tgtggggctg cacatccttg gtaaccacgc
     1261 tgcccaggcc cgttatggcc ccatccccca cttctatggg agcgcgcagc atcgtgtcgc
     1321 tgccgaggaa gacgttcttg cccacggtgg tcctgtgctt cttcacgccg tcgtaattgg
     1381 cggttatcgt gcccgccccg atattggtgc cctcgcctat cgtcgcgtcg cccacgtagc
     1441 tgaagtggtt catgttcacg ttcggctcga gcgtggagcg cacgacttca ccgaagttgc
     1501 ccatgcgcac gccttccatg aggtgcgcgc cggggcgcag gtggctgtag gggccaacgc
     1561 tgacattccg ttccaccgtg ctcccctcaa tcatggacgc gacgatgcgg cagttctcgc
     1621 caatcgtgga gttgatgatg tgggtattcg gccccagcgt gcagccggtg gctacgcggg
     1681 tggtgccttc gagtatcacg cccggataga gcaccgtgtc ggggtcaagg tgcacgtcca
     1741 tgcccaggta gatgctttcc ggcaggagca tggtcacacc ggcctcaagc cactggcggc
     1801 gcaggcggcc ctgtacgatc acctcggcct cggcaagctg ggcgcggctg ttgataccca
     1861 tcgcctcctc gatgccgtcg agggtgaaag tcagcaggcg gcgcttgcca cgcgccccca
     1921 tcgccagcga ggaagtggtg cccgtgccgc gctcctcttt gatcgccatg cctatcaggt
     1981 cggtcaggta gaactcaccg ctaggctggt tgggctgaag cccctggagg ctgttccaca
     2041 gccagtcaga ctcgaacacg tacacgccac tgttgatctc gtttatggcg cgctgcgtga
     2101 gcgaggcgtt gcgctcctcg accaccgcca gcacgttctg gtccttgtcg cgcatgaccc
     2161 gtccgtagcc ggtggggtcc tccactatgg cgctgagcat ggtgacgtgg gggcggttct
     2221 ggcgatgcct ggagaggagc tggttcaagg tgtcggtgcg gatgaggggc gtatcgccgt
     2281 acagtacgag cacggggccg tgatagctcc gcagggcctc ccgcgccatg agcacggcat
     2341 gtccggtgcc cagttgctcc ggctggttgg cgtaggtaat agagagggag ccgtcgagac
     2401 cgtacgagtt gccgagggtg gcctccacct ggtcggcacc gtgcccgacc actagcaccg
     2461 tgtggttggg ggcgaggggt gcgagggcgc gcaaaacatg ggcagcgagg ggtaggccgg
     2521 ccaggcggtg cagcaccttc ggctgccgcg actgcatccg cttgcccagc cccgccgcca
     2581 agactacaac cgagagcggc tggctctcca taagggttta tcctttgtca gagtatggtg
     2641 cgtgaggggc ttctactgag gtagccccat gttagcaggt gaggcagtgg ctgtcaagtt
     2701 tcagcagcct cgtaataacg acagctttag agacgaaaac gcaaagcatg tcctgagcgt
     2761 agccgaaggg acgcaaagga cgcaaagaac gcgcagagat tacgattgtt gttctaagta
     2821 cagaggggac cagggtgcga cagcacctac cctggtcc
//
LOCUS       DASRWZ010000145         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_491912, whole genome shotgun sequence.
ACCESSION   DASRWZ010000145 DASRWZ010000000
VERSION     DASRWZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491912"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFH60_02105"
     CDS             <1..197
                     /locus_tag="VFH60_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6312602.1"
                     /translation="SESATQVEPGTTVAQLWDALQTQYPPLRPLGSVSAFAVNGHYAT
                     PATPLSEGDVVAFIPPVSGG"
     gene            199..660
                     /locus_tag="VFH60_02110"
     CDS             199..660
                     /locus_tag="VFH60_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET6312603.1"
                     /translation="MALETTPHSGPQDVRCLFDITRNPLSTDELVNAVLVGSIGAIVT
                     FTGTVRDNNEGRKVVALEYEAYDEMAVAEMARIGSEMVAKWGLHGIAIRHRVGRLSIG
                     EASVVIAASSPHRREAFEACSEALDLLKARVPIWKKEYFEDGEHWVGIGAG"
     gene            737..1747
                     /gene="moaA"
                     /locus_tag="VFH60_02115"
     CDS             737..1747
                     /gene="moaA"
                     /locus_tag="VFH60_02115"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET6312604.1"
                     /translation="MEEFTDQLNRPLHDLRISVTDRCNFRCTYCMPKEVFGRDFQFLP
                     SADLLSFEEITRLARVFIGLGVEKLRITGGEPLVRRDLEKLIAMLAPLEGLKDLTLTT
                     NGALLAKKAPALKAAGLQRLTVSLDSLDDQVFRAMNDVDFPVGQVLEGMEAAREAGLG
                     PIKINMVVKRGVNDTSVLPMARHFHGTGYILRFIEYMDVGTTNGWRMDDVVPASEIVR
                     MIDAEMPLEPAERNYVGEVASRYRYLDGGGEIGIISSITQPFCSTCTRARLSPEGRLF
                     TCLFGNFGHDLRALLRTGKTDEEISALVAKIWRRRTDRYSDQRTLETVRRSKVEMSHI
                     GG"
     gene            complement(1850..2290)
                     /locus_tag="VFH60_02120"
     CDS             complement(1850..2290)
                     /locus_tag="VFH60_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET6312605.1"
                     /translation="MRRHHRLIFVNTSGWLALYNPNDPNHAAARMLWDDLRNQPVRFV
                     TTDYVLDQVFTAMKFFGSLDAAQALNTVINNTSLLRFFMTDSVIFERAWKVFVSDEHP
                     QFTFTDCINYAVIQYLGADEVFTFDRNFSAPGLMILPSPGGGDR"
     gene            complement(2290..2556)
                     /locus_tag="VFH60_02125"
     CDS             complement(2290..2556)
                     /locus_tag="VFH60_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312606.1"
                     /translation="MSNYLIDVHLYMEKGMYERLVDRAQELGVPLSAVVREAVVEYFA
                     NSPEVPDRSSDMPQPEDAIWQIPTLSGSFGALNVPGMGGQGGNS"
BASE COUNT          498 a          850 c          773 g          447 t
ORIGIN      
        1 catccgagag cgccacccag gtcgagccgg gcaccaccgt cgcccagctc tgggacgccc
       61 tgcaaaccca atacccgccc ctgaggccgc ttggctccgt gtccgcgttc gccgtcaacg
      121 gccactacgc cacgcccgcc actcccctct ctgagggcga cgtcgtggcc ttcatccccc
      181 cggtaagcgg aggctgatgt ggccctcgaa acgaccccgc actccggccc ccaggacgtg
      241 cgctgcttat ttgacataac ccgcaacccg ctatccaccg acgaactggt gaacgccgtc
      301 ctggtcggca gcatcggggc gatagtgacc ttcaccggca ccgtgcgcga caacaacgag
      361 ggccgcaagg tggtggccct cgaatacgaa gcctacgacg agatggccgt agccgagatg
      421 gcgcgcatcg gcagcgagat ggtggccaag tggggcctgc acggcatagc gatccgccac
      481 cgggtcggca ggctgtcgat aggggaagca agcgtggtca tagccgcctc ctccccccac
      541 cgccgggaag ccttcgaggc gtgcagcgaa gccctcgacc tcctgaaagc ccgcgtgccc
      601 atctggaaga aagagtactt cgaggacggc gaacactggg taggaatagg agctgggtga
      661 tttggaattt cggaatgcgg atttcggatt tgctccgtat ccaactaaat ccgaaattcg
      721 aaatccgaaa ttccaaatgg aagaattcac cgatcaactg aacaggcccc tccacgacct
      781 gcgcatctcc gtgacggaca ggtgcaattt tcgctgcacc tactgcatgc ccaaggaggt
      841 gttcgggcgc gacttccagt tcctgccgag cgccgacttg ctgagcttcg aggagattac
      901 ccggctggct cgcgtgttca tcggcctggg ggtagagaag ttgcgcatca cgggcggaga
      961 gccgttggtg cggcgcgacc tggaaaagct catcgccatg ctcgccccgt tagaggggct
     1021 gaaagacctc accctcacca ccaacggcgc gctgctcgcg aagaaggccc ccgccctgaa
     1081 ggcagcaggg ctgcaacgcc tcaccgtgag cctcgactcg ctggacgacc aggtgttcag
     1141 ggccatgaac gacgtggact tcccggtcgg gcaggtgctg gagggtatgg aggcggcgcg
     1201 ggaagcgggg ctggggccta tcaagatcaa catggtggtc aagcgcggcg tgaacgacac
     1261 aagcgtcctg ccgatggccc gccacttcca cggcacaggc tacatcctgc gcttcatcga
     1321 gtacatggac gtgggcacca ccaacggctg gcgcatggac gacgtggtgc ccgcctccga
     1381 gatcgtgcgc atgatagacg ccgaaatgcc cctcgaaccc gccgagcgca attacgtggg
     1441 cgaagtggcc tcccgctaca ggtacctcga cggcggcggt gaaatcggca tcatctcctc
     1501 gattacgcag cccttctgct ctacctgcac ccgcgcccgc ctctcccccg aaggccggct
     1561 attcacctgc ctgttcggca acttcggcca cgacctccgc gccctcctcc gcaccggcaa
     1621 gaccgacgag gagatatcgg ccctcgtagc caaaatctgg cgtcgccgca ccgaccgcta
     1681 ctccgaccag cgcaccctgg aaacagtacg acggtcgaag gtggagatgt cgcacatagg
     1741 cggctaacgc gtaacgcgtg acgcgtaaca cataacacgg ctccaggggc tttgttccct
     1801 gagagccgct ttgttgctta ttgcaccctt acctgttacg cgttacgcgt tacctatccc
     1861 caccacccgg cgaggggagg atcatcagcc ccggcgcgga gaagttgcgg tcgaaggtga
     1921 acacctcgtc tgccccgagg tactggatga cggcgtagtt gatgcagtcg gtaaaggtga
     1981 actgcgggtg ctcgtcgctc acgaacacct tccaggcccg ctcgaagatg acgctgtccg
     2041 tcatgaagaa gcgcagcagc gaggtgttgt tgataaccgt gttcagggcc tgggcggcat
     2101 cgagcgaccc gaagaacttc atcgccgtga acacctggtc cagcacgtaa tcggtagtca
     2161 cgaagcgcac cggctggttg cgcaggtcgt cccagagcat gcgggcggcg gcgtggttgg
     2221 ggtcgttggg attgtacagg gccagccagc cgctggtgtt cacgaatatc agccggtggt
     2281 gtcggcgcat tagctattgc ctccctggcc gcccataccc ggcacgttga gcgcgccgaa
     2341 cgagccgctc aatgtgggta tctgccaaat cgcgtcttcc ggctggggca tgtcgctgct
     2401 gcggtcgggc acctccgggc tgttggcgaa gtactccacc accgcctcgc gcaccaccgc
     2461 gctgagcggc accccaagct cctgcgcccg gtccaccagc cgctcgtaca tccccttctc
     2521 catatagaga tgcacgtcaa tcaggtaatt actcactcat ctcctcct
//
LOCUS       DASRWZ010000146         4184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1245808, whole genome shotgun sequence.
ACCESSION   DASRWZ010000146 DASRWZ010000000
VERSION     DASRWZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245808"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFH60_02130"
     CDS             complement(<1..256)
                     /locus_tag="VFH60_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312607.1"
                     /translation="MADTNNRDNTGNTQPGDHSGTTGTQAVAEPQSSAPTVPPAPALP
                     PMNPIERPGPTSEEWPEEQRRVLLVAAHPDDPEFSSAGTVA"
     gene            370..780
                     /gene="folB"
                     /locus_tag="VFH60_02135"
     CDS             370..780
                     /gene="folB"
                     /locus_tag="VFH60_02135"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET6312608.1"
                     /translation="MSVTNNHPTEPGEIRLSNMVFFGTHGANPEETALGQRFSVNLSF
                     WLDMSASSRTDQLEDTVSYSAVYKLVRSEVEGEPSKLLEHLAGRILDRILQFDGRVTR
                     ARVEVGKPSPPLKGSTTVEVWVVLERVRAGEVGT"
     gene            1026..1436
                     /locus_tag="VFH60_02140"
     CDS             1026..1436
                     /locus_tag="VFH60_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6312609.1"
                     /translation="MQQPDRPPGRNRSGALNEPKDVLVVEDEAYLCDLVADVLEAEGH
                     QARTASNGHEALERVQDRKPQLILLDLMMPVMDGWEFIRALRSNRDWADIPIVVVTAV
                     YDIKRTQQETGAVAVVTKPFDIDQIADVVARFSS"
     gene            1946..2962
                     /locus_tag="VFH60_02145"
     CDS             1946..2962
                     /locus_tag="VFH60_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312610.1"
                     /translation="MQWGIGPTLPIASPEGRISFIRGIEGTVPGLFVVNPDGSNLQQL
                     TRDIYIDQSAWSPDGKYIAAQVRYNNLGSILRVEVGADNKAVEIINLTDQETAENAFP
                     TWSPDGTRIAFMSKRGGGDWQIHVMNADGSDKRMVTDGKGLAKLPVWSPDGASIAYVL
                     SDGASSASEINVVPASGGAARAITSNGSVKSNPLWAHDGKSIIFVQGSGDRDNTIAVV
                     PVEGGEPRDITEPGAVKGLQLSPTEPVLVYYRILVESRGTDVYTLPLAGGPPTVVTTD
                     NTDDYLPAWSPDGKRLTWASSRAGRQGQEYRIAVGNPDGTGTQVITSGPGAEYQPQWA
                     PAVK"
     gene            2970..>4184
                     /locus_tag="VFH60_02150"
     CDS             2970..>4184
                     /locus_tag="VFH60_02150"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6312611.1"
                     /translation="MPRTQTRRNAFNAILLGMAAMLLWACSDLPVSTGPTPTTVALQA
                     TRTPRPTRVAPTPTEELFIEDPTETPEEEPAATPTPRKSLGGGGSGPTATEEAPRSTR
                     TPRTTPTAVAGEATPIATLMPSADRLAIFEQVWETVADNYLYEDFHGADWDALKEEYE
                     PKVRAATSGDEFYATVDDMVSELGDDHSRFISPWDADEEDDLMSGNTSYVGVGIFSKH
                     NETEIQVVYVFPGSPAEEGGIQRRDVITAVDGGPITPADEDLSRIRGPEGSKVTLTVR
                     SPGEEPRDVELVRRPINGAVLPSARWLEADPSVGYLVIPSFDPEDMDEQVADELATLV
                     EGDEPLAGIVVDLRGNGGGLIDTMQRIVGQFMTGEAGKYASRGREHMMVPPRGTMYQQ
                     LKDIPLVVLVDEG"
BASE COUNT          845 a         1290 c         1318 g          731 t
ORIGIN      
        1 gggccacggt gcccgccgac gagaactccg ggtcatccgg gtgggcggcc acgagcagca
       61 ccctgcgctg ctcctccggc cactcctcgg aggttgggcc gggcctctcg atagggttca
      121 tcggcggcaa agcaggcgcg ggtggcacgg ttggtgctga actttgaggc tcggcaaccg
      181 cctgtgtgcc ggtagtgccg ctatgatcgc ccggctgcgt gttccctgta ttgtctctat
      241 tgttggtgtc ggccatatgg atgggtcgct ccctttttgc gtagtgtgct tgtccctaag
      301 tgcggctggg cacgcctcca cggtataaca ctcctttatc gcagcgtcaa gcaacgggat
      361 agatgaccca tgagcgtaac gaacaaccat ccgaccgagc caggtgagat acggctgagc
      421 aacatggtct ttttcggaac gcacggcgcg aacccggaag agaccgccct cgggcagcgt
      481 ttcagcgtca atctgtcctt ctggctcgac atgtccgcgt cttcgcgcac ggaccaactg
      541 gaagacacgg tgagctattc cgccgtctac aagttagtcc ggagcgaagt agagggcgag
      601 cctagcaagc tcctggagca cctggcgggc cgtatacttg accggatatt gcagttcgac
      661 gggcgcgtaa cgagggccag ggtcgaggtg ggcaagccaa gcccgccgct caaagggagc
      721 actacagtgg aagtatgggt cgttctggag agagttagag cgggagaggt gggtacatag
      781 tgtccttacg gtccgatttt cgtgcctgag aaggccaaga actgccgcct gttggcacgc
      841 tacttgcacg agatcatcta ttcaggctat gtttaccggc ggcaaacata gcgagactta
      901 actaaggata agcgtcttcc cacatgtctc aacaatataa tgcttcccgc gagtacggca
      961 tgatcacgac tggcatgccg aggtcgcgtt atatcttgcg gcctgagaca aggggcagtg
     1021 gcggaatgca gcaacccgat aggccccccg gccggaaccg cagcggcgct ctcaatgagc
     1081 caaaggacgt gctcgtcgtt gaagacgagg cgtacctgtg cgacctggtc gccgacgtgc
     1141 tggaagccga agggcaccag gcgcgcaccg cgtccaacgg ccatgaggcg ctggagaggg
     1201 tccaggaccg caagccacag ttgatcctcc tcgacctgat gatgcccgtc atggatggct
     1261 gggagtttat tcgcgcgctc cgctccaacc gcgactgggc cgacatcccc attgtggtgg
     1321 tcaccgccgt ctacgacatc aagcgcaccc agcaggagac cggcgcggtg gccgtcgtca
     1381 ccaaaccctt cgacatcgac cagatagcgg acgtggtagc gcgctttagt tcctgacagg
     1441 ccatgaggcc agggtaacgc caagattagg attattcacc gcagagacgc agagagcgca
     1501 gagggtgcgc tgagattgcg gaaggtctaa tgagcgcaag gggcagcatc ttattgtgag
     1561 atgctgcccc ttccacatac caacctcaat tcttctctgc gtaacctctg cgtgctctgc
     1621 gtctctgcgg tgaactcttt gttccccctg gcgtggctcc cggtatcctg ccgtctagct
     1681 tctgttgctt gaggcactgc caccgggtgc gctataatag actcaggccc cgcgcgctcg
     1741 catgcccatc ccgtgcgggg ctagactctc tacccgagca aaccccgata tgagtaatag
     1801 ttccaggcgg tcttctcgcg cttcaaggtc gcagcacgtg gccgcgcgta ccactaccct
     1861 attggttggc gccggtgtcc tgaccgcgct agtccttgca tatgtgacgc tgcggttcgc
     1921 ggcccctagc gtgctgcctc cctttatgca gtggggcatt gggcctaccc tgcctatcgc
     1981 ctcccctgag ggccgaatca gcttcatccg cggcattgag ggcacggtgc cgggcctgtt
     2041 cgtggtcaac cccgatggca gcaacctgca acagcttacc cgcgacatct acatcgacca
     2101 atcggcatgg tcgcctgacg gcaagtacat agcggcccag gtcaggtaca acaatctggg
     2161 aagcatttta agagttgagg ttggggctga caataaggcg gtggaaataa taaacctcac
     2221 cgaccaggag accgccgaga acgcctttcc tacgtggtcg cctgacggca cgcgaatcgc
     2281 tttcatgtcc aagcggggcg gcggtgactg gcagatccac gtgatgaacg ccgacggctc
     2341 cgacaagcgc atggtcaccg acggcaaagg gctggccaag ctgccggtat ggtcgcccga
     2401 cggcgcgagc atagcttacg tgctgagcga cggcgcaagc tccgcgagcg agatcaatgt
     2461 ggtcccggcc tccgggggcg cggccagggc tataactagc aacgggtcgg tgaagtcgaa
     2521 cccactctgg gcgcacgatg gcaagtctat aatatttgtg caaggctccg gcgaccgcga
     2581 caacactata gccgtcgtgc ccgtggaggg tggcgaaccg agagacatta cagaacccgg
     2641 cgctgtgaag ggtctgcaac tatcccccac cgagccggtg cttgtgtatt acaggatact
     2701 ggtcgagtcg aggggcaccg atgtatacac gctccccctg gccggtgggc ctcccacggt
     2761 ggtcacgacc gacaacaccg acgactatct tcccgcgtgg tcgcctgatg gcaagaggct
     2821 cacgtgggca agcagccggg caggccgcca gggccaggaa taccgaattg cagttggcaa
     2881 ccccgacggc acaggcacgc aggtgataac ctccggtcca ggggccgaat accagcccca
     2941 gtgggcacca gcagtaaagt aggagtaaga tgccgcgaac tcagaccagg cggaacgcct
     3001 tcaacgccat actgctaggt atggccgcca tgctcctgtg ggcctgtagc gacctcccgg
     3061 taagcacagg ccccacaccc acgaccgttg ccttgcaggc gaccaggacg ccgcgcccca
     3121 ctcgtgtggc ccccacgccc acagaagagc tattcatcga ggaccccacc gaaacgccgg
     3181 aagaggagcc tgccgccaca cccaccccgc gcaaatcgct gggcggcggg ggaagcggcc
     3241 ctacggctac ggaggaggcc ccgaggagca cccgcacccc caggacgacc cctaccgccg
     3301 tagccggaga agctactcct atcgcgacac tcatgccctc cgccgaccgg ctggcgattt
     3361 tcgagcaggt ctgggagacg gtggccgaca actacctcta cgaagacttt catggggccg
     3421 actgggacgc gctcaaggag gagtacgagc caaaggtccg cgcagccacg tccggggacg
     3481 agttttacgc cacggttgac gacatggtct cggagctggg ggacgaccat tcacgcttca
     3541 tctcgccctg ggacgccgac gaagaagacg acctgatgag cggcaatacc agctacgtgg
     3601 gcgtgggcat cttcagcaag cacaacgaga ccgaaatcca ggtggtttac gtcttccccg
     3661 gcagcccagc cgaggaaggt gggatccaga ggcgcgacgt aatcaccgcg gtggatggcg
     3721 gccccatcac accggcggac gaggacttga gccgcatcag agggccggag ggcagcaagg
     3781 tgacgctcac cgtgcgctcg cccggcgagg agccgcgcga tgtggagttg gtccgcaggc
     3841 cgatcaatgg cgctgtgctc ccctccgctc gctggctcga agcagacccc tcggtggggt
     3901 acctcgtcat accgagcttc gacccggaag atatggacga gcaggtagcg gacgagcttg
     3961 cgacgctggt tgagggcgac gagccgctgg caggcatagt ggttgacctg cggggcaacg
     4021 gcggcggcct gattgacacg atgcagcgca tcgtcggcca gttcatgacc ggggaggcgg
     4081 ggaagtacgc ctccaggggc cgcgagcaca tgatggtccc gccacgaggc accatgtacc
     4141 agcaactgaa ggacatcccc ctcgtggtgc tcgtggacga aggc
//
LOCUS       DASRWZ010000147         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_560825, whole genome shotgun sequence.
ACCESSION   DASRWZ010000147 DASRWZ010000000
VERSION     DASRWZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560825"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFH60_02155"
     CDS             complement(<1..219)
                     /locus_tag="VFH60_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET6312612.1"
                     /translation="MHAHSRHATSEDQRTREKNWAALSSMFVAVALTAAKLGAGLISG
                     SLGLLSEALHSAMDLVGTVLSYAAVRISN"
     gene            399..1232
                     /locus_tag="VFH60_02160"
     CDS             399..1232
                     /locus_tag="VFH60_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6312613.1"
                     /translation="MLARARQSILYRDLLYNLVVRDLKVRYKHSFLGFFWSLLNPLLL
                     MAVFTFVFTTLLGQSGGAYPMYHIFFLCALLPWNWCASSVVGTLGSIVNNGHLIKKVY
                     FPREFLPFSTVVSNMINYLLSLPALLVFMLLFRSPEAGNPFSADCHLACINEHMLWLP
                     LLILTQAVFLVGLGFFLAALNVFFRDTSVLVEVGLNAWFFLTPIIYDVTTLTSFPAVM
                     YYLNPMASIITNYRDIFYHNQPGSPDVLFMLRTLAECTVILVAGYLFFMRTSRTFGEE
                     I"
     gene            1235..>2654
                     /locus_tag="VFH60_02165"
     CDS             1235..>2654
                     /locus_tag="VFH60_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6312614.1"
                     /translation="MSRTIEQPEQGSTSKPQPNGSKAGASNGRKLLAESGRPMSSGAP
                     GGGADKPYKVIKFSKVSKRFILRHERPRSFQDVLVSLFGLRSLSKRGVAMPRPANEEF
                     WALRDVNFSIYAGEAVGIIGENGSGKSTTLKLISRILEPSTGSVSVRGKVSALLELGT
                     GFHPELTGRENVFLNGSLLGLSRKEMASRYDAIVDFSEIGEFIDTPIKPYSSGMVMRL
                     GFAVAINVDPDILLTDEVLAVGDEAFQRKCLDYIARLRSRGVTIVFVSHALEAVRSLC
                     SRAIWLDHGRMIADGPAGQVVDQYLTYENDKHAERLKAGQESRPPNTITAASRPQPMT
                     TPLPDEDEEDLLPEGDGGPLRADRTAPLETPDVKITGVTFLDGSGEPSQVFYTGQPIT
                     IRLHFNARARVEDPVFGLALYTENGTHINGPNTRFSGLVIPAIEGTGYADYHVEQLPL
                     MAGRYYVTVAVTGREFTNILDHQ"
BASE COUNT          476 a          861 c          772 g          545 t
ORIGIN      
        1 gttcgagatg cggactgcgg catagctgag caccgtgccc accaggtcca tagctgagtg
       61 tagcgcctcc gagagcaagc cgaggctgcc ggaaatcagg cccgcgccca gcttcgccgc
      121 cgtcagggct accgccacga acatggagga gagcgccgcc caattcttct cgcgggtgcg
      181 ctggtcctca ctcgtggcgt gtcggctgtg cgcgtgcatg ctgccctctc ctctacggtg
      241 gccggtctaa tgggtattat ctcacatcgc gtgccactgg cggcgagtgt gccttcgctt
      301 gacacccccc ggtgccgctc ttataatccc tgacagaacc tacctcgcgc acggccgtta
      361 ttactgcata ctaaatcccg gattcccagg ccctcatttt gctagcaaga gcacgtcagt
      421 caattctata ccgcgacctg ctgtacaacc tcgttgtacg ggacctcaag gtccgttata
      481 agcactcctt cctcggcttc ttctggtcct tgctgaaccc gttgctgctt atggcggtgt
      541 tcacgttcgt cttcaccacg ctgctgggcc agagcggcgg cgcgtacccg atgtaccaca
      601 tcttcttcct gtgcgcgctg ttgccctgga actggtgcgc tagctccgtc gtgggcacgc
      661 tcggcagcat cgtgaacaac gggcacctta tcaagaaggt ctatttcccg cgcgagtttc
      721 tgcccttctc tacggtcgtt tcgaacatga tcaactacct gctgtcgctc cccgcgctgc
      781 tggttttcat gctcttgttc cgctctcccg aggcgggcaa ccccttcagc gccgactgcc
      841 acctggcgtg cataaacgag cacatgctct ggctgcccct cttgatactc acgcaggcgg
      901 tgttcctggt agggctcggt ttcttcctgg cggcccttaa cgtctttttc cgcgatacaa
      961 gcgtgctcgt cgaagtgggc ttgaacgcct ggttcttcct cacgcccatc atctacgacg
     1021 tgactaccct aaccagcttc cccgcggtca tgtactatct gaacccgatg gcttccatca
     1081 tcaccaacta tcgcgacatc ttctaccaca accagccggg cagtcccgac gtgctgttca
     1141 tgctgcgaac gctggcggaa tgtacggtca tcctggtcgc gggctacctg ttcttcatgc
     1201 gtaccagccg cacatttggt gaggagattt agcggtgtct cgtacgatag agcagcctga
     1261 gcaggggagc acctctaagc cgcagccgaa cggctcgaag gccggtgcct ctaacggcag
     1321 gaagctattg gcggagtctg gccgtcccat gtcgtcaggt gcgcccggcg gtggggccga
     1381 caagccgtac aaggtcatca agttcagcaa ggttagcaag cggttcatct tgcgccacga
     1441 gcgcccccgc tcgttccagg acgtgctggt gagcctgttc ggactgcgca gcctctccaa
     1501 gcgcggtgtc gccatgcccc gccccgccaa tgaggagttc tgggccttgc gggacgtgaa
     1561 tttcagcatt tacgcgggcg aggccgtcgg catcatcggg gagaacggtt ccggcaagtc
     1621 caccacgctc aagctcatct cgcgcatact ggagccgtcc acggggagcg tatcggtgcg
     1681 cgggaaggtg tcggccctgc tggaactggg caccggcttt cacccggaac ttacaggacg
     1741 ggagaacgtc ttcctgaacg gctcgctgct cgggctttcg cgcaaggaaa tggcgtcccg
     1801 ctacgatgcc atcgtggact tctccgagat cggggagttc atcgacacgc cgataaagcc
     1861 ttattcgtcc gggatggtta tgaggctagg tttcgcggtc gccatcaacg tggacccgga
     1921 tatcctgctc accgacgagg tgctcgcggt gggagacgag gctttccagc gcaagtgcct
     1981 cgactatata gcgaggctgc ggagtcgcgg ggtgactatt gtgttcgtgt cgcacgcgtt
     2041 ggaagcggtg cgctcgctgt gcagcagggc catatggctg gaccacggcc gcatgatcgc
     2101 ggacggccct gcggggcagg tcgttgacca gtacctgacg tacgagaacg acaagcacgc
     2161 cgagaggcta aaagccgggc aggagagccg tccccccaac accatcaccg cggccagccg
     2221 cccccagccg atgacgaccc cgcttcccga cgaggacgaa gaggacttac tgccggaagg
     2281 ggatggtggc ccactgcgtg ccgacaggac cgcccccctc gagactcccg acgtcaagat
     2341 caccggcgtt accttcctcg acggcagcgg ggagccttcg caagtcttct atacgggcca
     2401 gccaatcacg attcgactgc acttcaacgc cagggcgcgt gtcgaagacc cggtgttcgg
     2461 ccttgcgctc tatacggaga acggcaccca tatcaacggc ccgaacacgc gcttctccgg
     2521 gcttgtaatc cctgcgatag agggcacagg ctacgctgac taccacgtcg aacaactgcc
     2581 cctgatggcc gggcgctact acgtgacggt cgctgtcacc ggacgcgagt tcaccaacat
     2641 tctggaccac cagc
//
LOCUS       DASRWZ010000148         3961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_149451, whole genome shotgun sequence.
ACCESSION   DASRWZ010000148 DASRWZ010000000
VERSION     DASRWZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149451"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..2203
                     /locus_tag="VFH60_02170"
     CDS             422..2203
                     /locus_tag="VFH60_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET6312615.1"
                     /translation="MENIDLLVDLAFAMGAALLGGFIAHLLRQPALLGYLLAGVAIGP
                     YTPGPVTSVERVQTLANFGVALLMFALGTEFSLEALQRVRRVAVWGGLGQIVLVIALG
                     TVMGLLMGFSVTHSIFLGGVISICSSILMLKLLIPRGEVESIAGRAALGTSIVQDLAT
                     VALIIVLPALAGEIGPALLADAGIAILRGGAFLAAAYFLGTRLVPPLLEQVARAGARE
                     LFLLTVVAIAVGMAVLGHLAGISFALGAFIGGLVVSESEFSSEVLDEIIPVRDIFSTL
                     FFVSIGMLMEPAFLLSHSVQIALLVATIIVGKFVIASLLVRLLGYDTENALRTGLLLA
                     QIGEFSFVLAGVGLVSGTIDEELYGLILAAALVTLVLNPILVNSSDRFIAPLSRPVER
                     LIRRLPGAAARLPVDAVVREVVADDTQLAALRRHVVLCGFGRVGREVARVLEDRPFPF
                     VVIEYNPRRVEEARHLGYLAIQGDATDTATLERAGIAGAYILVVAVPDLVSAEQIVRQ
                     GRAMSRKVYIIARSTDTRAIPHLMRAGADQVLQPEFETGLEIIRQVWRKYGVSSIETQ
                     SMIGGQRRRHYSGEITEHDYTDDPFWS"
     gene            complement(2378..3751)
                     /locus_tag="VFH60_02175"
     CDS             complement(2378..3751)
                     /locus_tag="VFH60_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET6312616.1"
                     /translation="MDSLVFNLWYLVAGALLVVMALGGSVLKRLPLTTSILYLAVGFA
                     LGQHGAGLIDIDPLEESGLLERLTEVAVIISVFTAGLKLRAPLSRPRWFIPVRLAFLS
                     MTITVGLITLVGVAVLGLPVGVAVLLGAILAPTDPVLASDVQLEHPFDRDKLKFGLTG
                     EAGLNDGAAFPFVMLGLGLLGLHDIGNFGWRWFAIDVVWAITGGLAVGGLLGTIVGRL
                     VIYLRKNHKEALGLDDFLALGLIALSYGVALMAHTYGFLAVFAAGLALRRIEMLSSGD
                     KPPEEVMAVTRAVENEEKEEIATDPEKASAYMAEAVLGFNEQLERIGEVGVVVLVGGL
                     LTWDLLPLEAWWFVPLLFLVIRPISVEVGLIGSSVAPLQRRLMSWFGIRGIGSVYYLM
                     YAIHNGVPEEASQQLLSLVLTVVAVSVVVHGLSVTPLMGLYGRLTSRERDGTAKTPRT
                     PRTTPSK"
BASE COUNT          744 a         1286 c         1213 g          718 t
ORIGIN      
        1 ttgaccgtca caatcgttgc aacggtattc ctatcgctcc catcatcctg agcgttgaag
       61 actctagcct acccacctga tccttcgcta ggctcaggat gacaaagcca atttggtcct
      121 cccggaacgg tgcatgtagc tacaccagcg caccatatgt agtgctcacc tgccagaaac
      181 gttagcactc tttgggttct ttgcgtattg gcgtttccgt ctccttgccc ctatctgagg
      241 catattactt gctgacgcag gggcaaaccg gctgttcagc cccggcgacg gcctgcacag
      301 cgcatctcct ccgacctaaa aagcacacgt ttcatcgcag caacctgggt accttgaggc
      361 actcagtgat agcgcatacc cggcaccggc cgcatacggc ccaggaaatt acccgacccg
      421 catggaaaac atcgacctgc tagtagatct cgcattcgcg atgggcgcgg ccctgctggg
      481 agggttcatc gcgcacttgc tcaggcagcc cgccctcctg ggctacctgc tggcaggcgt
      541 tgccatcggc ccttacaccc ccggccccgt cacgagcgtg gagcgggtgc agacgctggc
      601 gaacttcggc gtggcgctcc taatgttcgc gttgggcacc gagttctccc tggaggccct
      661 gcaaagggtg cggagggtgg cggtgtgggg cgggctgggg cagattgtgc tcgtaatcgc
      721 gctcggcacc gtgatgggcc tgctgatggg cttctccgtc acgcactcga tcttcctggg
      781 cggggttatc tctatctgta gctccatcct catgctgaag ctgctcatac caagagggga
      841 ggtagagtct atcgcgggca gggcggcgct gggcacgagc atcgtgcagg acctggcgac
      901 cgtggctttg ataatcgtgc tccccgcgct tgcgggcgag ataggtccgg cgctgctggc
      961 cgacgcgggt atcgccatcc tcaggggcgg ggcgttcctg gctgcggcct acttcctggg
     1021 gacgcgcctg gtgcccccgc tgctggaaca ggtggcgcgc gctggggcga gggagctatt
     1081 cctgctcacg gtggtggcta tcgcggtagg catggcggtg ttgggccacc tggcggggat
     1141 ctcgttcgcg cttggggcct tcatcggagg tctggtggtc tccgaatcgg agttcagcag
     1201 cgaagtgctg gacgagatca tcccggtgcg cgacatattc tcgaccctct tcttcgtgtc
     1261 catcggcatg ctcatggagc ctgccttcct cttaagccac agcgtgcaga tagcgctcct
     1321 ggtagccacc ataatcgtag gcaagttcgt aattgccagc ctgctcgtcc ggctgctggg
     1381 ctacgacaca gagaacgcct tgcggacggg gctgttgctg gcgcagatag gcgagttctc
     1441 gttcgtgctt gccggggtcg gcctggtgag cggcacgatt gatgaggagc tttacggcct
     1501 catcctggcc gccgccctgg tgacgctggt gctgaaccct atcctcgtca acagctcgga
     1561 ccgctttata gcgccgctca gcaggcccgt cgagaggctc atcaggcggc ttcccggcgc
     1621 ggcggcacgc ctgcccgtgg acgctgtagt gcgggaggtg gtggccgacg acacgcaatt
     1681 agcggcgctc cgcaggcacg tggtcctctg cggcttcggc agggtggggc gcgaggtagc
     1741 gcgggtgctg gaagaccggc cgttcccgtt cgtcgtcatc gagtacaacc cgcgcagggt
     1801 agaggaggcg cgacacctgg gctacctggc gatacagggc gacgccaccg acactgcaac
     1861 attggagcgg gcgggcatcg ccggggctta catactcgtg gttgcggtgc cggacctggt
     1921 gagcgcggag caaatcgtgc ggcaagggag ggctatgagc aggaaggtgt acattatcgc
     1981 gcgcagcacg gacacacgtg ccatccccca cctcatgagg gccggggccg accaggtatt
     2041 gcagccggag ttcgagaccg gcctcgagat catcaggcag gtatggcgca agtacggcgt
     2101 ctcctctatc gagacccaga gcatgatagg cggccagcgc aggcggcact acagcggcga
     2161 aatcaccgag cacgattaca ccgacgaccc cttctggtcg tgagtatagc aagagaccga
     2221 accgccaagc atgccctgag cgtagccgaa gggacgccaa ggacgccagg agagcgccaa
     2281 gtctaaatag gtgttcttga gcaagaagac cagggtacga aactatgtat tccggtgctt
     2341 ttcatgtacc ctggctcttc ttggactctc caaaccttta cttacttggc gtggtccttg
     2401 gcgtccttgg cgtcttggcg gttccgtctc tctctctcga cgtcagcctc ccgtaaaggc
     2461 ccatcaaggg ggtgacggac aggccatgca ccaccaccga caccgccaca acggttagca
     2521 ccagcgatag aagctgctgg gacgcctctt cgggcacacc gttgtgtatg gcgtacatga
     2581 gatagtagac agagcctatc ccccgtatgc cgaaccagga catgagccgc cgttgcagtg
     2641 gggccacgct ggagcctatc agccctacct ctaccgagat gggccggatc accaggaaca
     2701 gcaagggcac gaaccaccac gcctccaggg gcagcaggtc ccaggtcagc agcccgccca
     2761 ccagcaccac cacgcccacc tcgcctatgc gctcgagctg ctcgttgaaa cccaacaccg
     2821 cttcagccat gtatgcggag gccttctccg ggtcggtggc gatctcctcc ttctcctcat
     2881 tctccaccgc ccgcgtgacc gccatgacct cttcgggcgg cttgtcgcca ctagacagca
     2941 tctctatgcg gcgcagggcc agtcccgcgg cgaacacggc caggaagccg taggtgtgcg
     3001 ccatgagagc caccccgtac gagagggcga tgaggcccaa cgccaggaaa tcgtcgaggc
     3061 ccaacgcctc cttgtggttc ttgcgcaggt agatcaccag ccggccaacg atcgtgccca
     3121 gcagcccccc aacggcaagc ccacctgtga tggcccacac cacgtcaatt gcgaaccagc
     3181 gccagccaaa attgcctatg tcgtgcaggc ccagcaggcc cagccccagc attacgaagg
     3241 ggaaggccgc gccgtcattg agacctgcct ccccagtcag cccgaacttc agcttgtcgc
     3301 ggtcgaaagg gtgctcgagc tgtacgtcgg aggcgagcac cgggtcggtg ggggccagga
     3361 tcgcgcccag gagcacggct acccccaccg gcagccccag cacagccacg cccaccaggg
     3421 tgattagccc caccgtgatg gtcattgaga ggaaggccag ccgcaccgga atgaaccacc
     3481 tcggccggct cagcggcgcg cgcagcttta gccccgccgt gaacaccgaa atgattacgg
     3541 ccacctccgt tagccgctcc agcaggcccg actcttcgag gggatcgatg tcgatgaggc
     3601 ccgccccgtg ttgccccaac gcgaacccca ccgccaggta caggatggag gtggtgaggg
     3661 gcagtcgctt cagcaccgag ccgccgagcg ccataaccac cagcagcgct ccggccacca
     3721 ggtaccacag gttgaaaact aagctgtcca atctactccg cctcgcgccc ctcttccggg
     3781 cgtctcaccg cctgcctgga ccctccacct ccaccacctg aaagccccat aagcggcagt
     3841 cccaacgata gaggcaagtt gcgtacccaa agtggacggc agcaagggtt ggcagctttc
     3901 cagaatatag tcattctggg aaagctgctc gcgccatagc cacgctgatg aggcagacta
     3961 a
//
LOCUS       DASRWZ010000149         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_217810, whole genome shotgun sequence.
ACCESSION   DASRWZ010000149 DASRWZ010000000
VERSION     DASRWZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217810"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(473..1687)
                     /locus_tag="VFH60_02180"
     CDS             complement(473..1687)
                     /locus_tag="VFH60_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HET6312617.1"
                     /translation="MSKPRNRLYLLIIMLFSILPVRGAEAQADSTSYRAETTRWRAAE
                     GQFAGWQGVGVSLAPDGALRLDRGKARGEAERYGAGRYRHGGGFVGETTGPVITTAFP
                     FSEAVPSWNASTPTGTWLEAQLRVRLGSRWTRWYNLGVWASDPETLARHSVSGQADAD
                     AYVDVDTLKLGRRGDPLTATAYQLKFRLFARGRDSLPSLTNAAVAVSTSPTAPTAPAP
                     GNPASWDNLLAVPECSQMVYPDGGEVWCSPTSVAMVLGYWEGRRGPCESGVRAAVSGV
                     YDSVYEGHGNWPFNAAYAASRGFESYVTRFTSMSQAEAWIAAGVPVIISYSWGRGQLT
                     GAPLPASNGNLAVLAGFDATGNPVVNDPAAPNNEAVQRTYSRAELERLWLQGSGGTAY
                     LIYPADRVVPDL"
     gene            2144..>2372
                     /locus_tag="VFH60_02185"
     CDS             2144..>2372
                     /locus_tag="VFH60_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6312618.1"
                     /translation="MTKKKREERTVRLGIIGAGLAIKLLHWSPLSRMPERFSVVAVAD
                     IDANAAQEVAGLASGCRSTTDYHELLASEDVE"
BASE COUNT          438 a          757 c          753 g          424 t
ORIGIN      
        1 acctcccctg cataagtgag agttgtcgat aagcaggaac cacctccttc atgagaagca
       61 aaaggagagc cgtgacgagg tgaggtgtgg ggaaggtgtt tcacggcagg cgctcgttac
      121 accgctacag cccagttcaa atggtgcata aggcgtaaaa agtactgcaa gaatgcggct
      181 caatgcatta tacgcccaca cccgaggctg tcaagcatct gcgcgacagg gggcgcagct
      241 atgcgcgtgg gcgaccagga ccacactccc ccttgtcatc ctgagcgaag cgaaggatca
      301 gccgcccgtg tttgactctt ggtattaatg caccagtgtt cctgatgcca ccagcatcgc
      361 ctacgttgaa gactctcgct tacccacctg atccttcgct tcgctcagga tgacaagggg
      421 ggcatggtcc tgtagttgtg ctgcgggccg cgacgttcgc taagacatcc gcttacaggt
      481 caggcactac tcggtcagcc gggtaaatca ggtacgccgt tccgccggaa ccctgcaacc
      541 acaaccgctc tagctccgcc cgcgagtagg tgcgttgtac ggcctcgttg ttgggcgccg
      601 cgggatcgtt cacaacgggg ttgcctgtgg cgtcgaaacc tgccagcacg gccaggttgc
      661 cgttgcttgc gggcaggggc gcgccggtga gttggcccct gccccagcta tagctgatta
      721 tcacaggcac gcccgccgca atccatgctt ccgcctggga catgctcgtg aagcgggtca
      781 cgtaggactc aaagccccgg ctcgccgcat aggccgcgtt gaagggccag ttgccgtgcc
      841 cctcgtagac gctgtcgtac acgccgctca ccgccgcccg cacgcccgac tcgcatggcc
      901 cccgccgtcc ctcccaatag cccagcacca tagctacaga agtggggctg caccacacct
      961 ccccgccgtc ggggtatacc atctgcgagc actcaggcac ggcgagcaga ttgtcccagg
     1021 acgcggggtt gccgggcgcg ggcgcggttg gagcggtggg gctggtcgag accgctacgg
     1081 cggcgttggt gaggcttggg agcgagtcgc ggcccctggc gaacagtctg aacttcagtt
     1141 ggtaagccgt ggcggtgaga gggtcgccgc gcctgccgag cttgagcgtg tccacgtcca
     1201 cgtaagcatc ggcgtctgct tgcccgctca cggagtgccg agccagggtc tccgggtcgc
     1261 tcgcccacac gcccaggttg taccacctgg tccagcgcga gcccaggcgc acccggagtt
     1321 gggcttcaag ccaggtgccc gtgggcgtag aggcgttcca ggaaggtacg gcttccgaaa
     1381 aggggaaagc cgtcgtgatg acagggccgg tggtctcgcc cacgaagccg ccgccgtgcc
     1441 tgtacctgcc cgccccgtac cgctcggcct ccccacgcgc cttgccccgg tcgagtcgca
     1501 aggcgccatc aggggcgagc gacaccccca cgccctgcca gcccgcgaac tggccctcgg
     1561 cggcacgcca gcgggtggtc tcggccctgt acgatgtgct atcggcctgg gcctcggcac
     1621 cccgcactgg caagatggag aaaagcatga ttatcaggag gtacagccgg ttgcgtggct
     1681 tggacacacg gttcctttcg acgggaaaag ggcagggcgg ttcgggcggc cgcgcttaca
     1741 gtatatcatg tcggggcgtg tggctgatgg ctcgccagga ggctactggg ttcatgttcg
     1801 ataggtaagt tggggctgcg ctggatgaga caccccgtga tagccctgtg gctaaagcca
     1861 cgggcttgcc agagacgaag tctcctacgg agactacctg atgttgctta gtcccctatg
     1921 ggactttgcc tcttgtaagc tcgccaattc attggcaggg catccccaca gggctatagg
     1981 ggcagcacct gagtggcatt gttcctgcat gcaacaccga acggccctac ctgacccctc
     2041 actgcacccg caagagcagt accccgcttg ggtgcccaca actacgatat ggcggccttt
     2101 aaacttgtac ggcgtccgca gccgcgaata ggacagacca cacatgacaa agaagaagcg
     2161 cgaagagcga acggttcgcc tcggtatcat cggggctggg cttgccatca aattgctcca
     2221 ctggtcgcca ctcagccgca tgcctgagcg cttcagcgtc gtcgccgtgg ccgacatcga
     2281 cgcgaacgcc gcgcaagagg tggccgggct ggcgagcggg tgccgttcga ccacggacta
     2341 ccacgagctg ctcgcctctg aggacgtgga gg
//
LOCUS       DASRWZ010000150         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_835085, whole genome shotgun sequence.
ACCESSION   DASRWZ010000150 DASRWZ010000000
VERSION     DASRWZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835085"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFH60_02190"
     CDS             <1..706
                     /locus_tag="VFH60_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6312619.1"
                     /translation="RAVFLDAGLTLIYSELSLPTLCYEVGREHGLRVSLPALEAAVPR
                     ASAFLHHTQRGDPDLWSSDDRVQRLWTGFYTAVYHEAGVEADEATLNACAGDVYKRYL
                     APGAWKLYPDVLPTLVGLRDRGYTVGVISDWGSALTGKVLLPLGVGPYIDFMVVSTVQ
                     REAKPGSGLYREALARAGVPARQAVHVGDNYITDVLGARSAGIEGVLLDREGARRDMA
                     LDCLRIESLEELLPLL"
     gene            864..1895
                     /locus_tag="VFH60_02195"
     CDS             864..1895
                     /locus_tag="VFH60_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6312620.1"
                     /translation="MKDYKQIWNNLSASFPEASYHVCCVEDEDEIRSNGQLTAAFLRE
                     VLEIGPDDRVLEIGCGVARIGRELAPYCGEWHGADISGNMIAYAAKRTEGMPNVHLYE
                     QPDNTLSIFPDNYFDCVYCTIVFMHVDKLEMFTYLRESFRVLKPGGRAYFDTYNILAP
                     QAWEEFRRIIDTYAPHKRPGHVSQFSTPQEMQKFMEEAGFESVFIDGESNKQLLVALG
                     RKPQATAEATDAVPTGIAPTALPHSANEGATPDEPPETSDNESATQLRAKIDRLNKAL
                     RGKEAATHELQAMMYGKNRYIEEVEAALDVKNKHIARLERIIQRQEKLLRALTVRVAL
                     RLRRTLRLP"
BASE COUNT          431 a          701 c          626 g          336 t
ORIGIN      
        1 ccgcgcggtc ttcctcgacg cggggttgac cctgatttac tccgagcttt cgctgcccac
       61 cctctgctac gaagtggggc gggagcacgg cctgcgggtg tcgctacccg cgttggaggc
      121 ggcggtgcct cgcgcctcgg ccttcctgca ccacacccag cgcggcgacc ccgacctgtg
      181 gtcgtccgac gacagggtgc aaaggctctg gaccgggttc tacaccgccg tctaccacga
      241 ggcgggggta gaggcagacg aagccacgct gaacgcctgc gccggcgacg tttataaacg
      301 ctatctcgcc cccggtgcct ggaagctcta ccccgacgtg ctgcccaccc tggtcggtct
      361 gcgcgacagg ggttacaccg tcggcgtcat atcggactgg gggagcgcgc tcaccggcaa
      421 ggtgctgctg ccgctgggcg tggggcctta tatcgacttc atggtggtat ccaccgtgca
      481 gcgcgaggcc aagcccggct ccggcctcta ccgcgaggcg ctggcaaggg ccggagtacc
      541 tgcccgtcag gccgtccacg tgggcgacaa ctacatcacc gacgtgctgg gcgcgcgctc
      601 cgcaggcata gagggcgtcc tgctcgaccg cgaaggcgca cgccgcgaca tggcgctcga
      661 ctgcctgcgc atagagtcgc ttgaggagtt gctgccgctg ctatagccct acggggacta
      721 aggaacatca tgtagtagtc tccgtaggag acttcgtctc tggtaagccc gccaatacat
      781 tggcagggcg tccccgcggg gcgagcgtgg cagggcaccc caaccgtacc ggagaatacg
      841 actacacaag tggaggggtt agagtgaagg actacaagca gatttggaac aatctcagcg
      901 cttccttccc ggaggcgagc taccacgtgt gctgcgtcga ggacgaggac gagattcgca
      961 gcaacggcca gctaaccgcc gccttcctgc gcgaagtgct cgaaatcggc cccgacgacc
     1021 gggtgctgga gataggctgc ggcgtggcgc gcataggccg ggagcttgcc ccctattgcg
     1081 gcgagtggca cggcgcggac atctcaggca acatgatcgc ctacgccgcc aaacgcaccg
     1141 agggcatgcc caacgtccac ctgtacgagc agcccgacaa caccctctcc atcttccccg
     1201 acaactactt cgattgcgtc tactgcacca tcgtcttcat gcacgtggac aagcttgaga
     1261 tgttcaccta cctgcgcgag tcgttccgcg tgctcaagcc ggggggcagg gcctacttcg
     1321 acacctacaa catcctcgct ccccaggcgt gggaggagtt ccgccgcatc atcgacacct
     1381 acgcccccca caaacgtccc ggccacgtca gccagttcag caccccccag gagatgcaga
     1441 agttcatgga ggaggcaggc ttcgagtcgg tgttcatcga cggcgagagc aacaagcagc
     1501 tccttgtagc gttgggccgc aagccgcaag ccactgccga agccactgac gcggtaccca
     1561 ccggcatcgc gcccaccgcc ctcccccact ccgccaatga gggagccaca ccggatgagc
     1621 cgccagagac gagcgataac gaaagcgcca cccagttacg cgccaagata gaccggctca
     1681 acaaagccct gcgaggcaaa gaggcggcaa cccacgagct acaagcaatg atgtacggca
     1741 agaaccgcta catcgaggag gtagaagccg ccctggacgt aaagaacaag cacatcgccc
     1801 gcctggagcg catcatccag cgccaggaga agttgctgcg cgccctcacc gtccgtgtcg
     1861 ccctgcgctt gcgccgcacc ttgcgcctcc cttagccagc actatagtaa gaaagcctaa
     1921 aataacgtgc acggtatgag catccaaagc cccgcacttg ccattctgag tggtgcatct
     1981 tgcaaataag catcaaaaag aggaccaact ccccctctgt catcctgagc gtagcgaagg
     2041 atcaggtggg tagggaagaa gctgtattgg actacccgac caggcttgcc gcca
//
LOCUS       DASRWZ010000151         5609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_629280, whole genome shotgun sequence.
ACCESSION   DASRWZ010000151 DASRWZ010000000
VERSION     DASRWZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629280"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1457)
                     /locus_tag="VFH60_02200"
     CDS             complement(231..1457)
                     /locus_tag="VFH60_02200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6312621.1"
                     /translation="MSLSAQSIQPRVAYTHDLPRTADLVIIGGGIVGAATAFFAARAG
                     LRPLVLERRPALCTLTTPASTGAFRAQFDNPEEIALVSESIALFANFAQATGLEGYDL
                     DMRKQGYLWLTTSEAGVVIQKELVARQHAWGLTDVEWFDGDEARRRFPYLSPDVIGAR
                     FRAGDGWLDVRRLTLGYAAAASTGAVFATNTTVTGFDVQGGRVGAVHTSRGSVSTGRV
                     VVAAGPFAGEVAGMAGLELPLSLVRRQKLVMPDVPEVPPWAPMTIDEETGAHWRPALR
                     GANLLRTSPGVPPGPPLEDVPISVDFVFGLLDPASEHSVARISPFWREVWARQTSHYY
                     LQAGQYSYTPDHKPFLGPTPIEGLYLNCGYSGHGIMASGGGSRIVVDTITGSLRPEDN
                     PFRLDRPLVGRHMDIL"
     gene            complement(1491..2477)
                     /locus_tag="VFH60_02205"
     CDS             complement(1491..2477)
                     /locus_tag="VFH60_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HET6312622.1"
                     /translation="MQPHKPTLGALRLVPEYHHRVWGGQHLQSNADKPIGEAWLVYEQ
                     DKVASGPHEGRTLADLTEEYGEALIGRRVMAQTGPRFPLLIKILDCADWLSLQVHPND
                     EQAAELEGPEHFGKTEAWYVLDAEPGARLISGIETGTSPESLAKAIREGKVVELAQYI
                     DVGKGDTILMHAGTVHALGPGLLIYEVQQTSDITYRIYDWDRPLEGGRKLHIEQSVAV
                     SNPEMKGEVTPAPKPLRDGQREQLVCCDYFTLELIGSETQPVELDTGGETFHALTVTE
                     GEIQVEGDGWSERLGQYDSVVLPASVGAYTLRPLGRSQALKSSVELTDDVRA"
     gene            3415..4611
                     /locus_tag="VFH60_02210"
     CDS             3415..4611
                     /locus_tag="VFH60_02210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6312623.1"
                     /translation="MSLEPVSIVAPGKRKHAEQIFELVATQWRDDLKRHAREGRINRS
                     HYDWKTSRVGIANGRVVAHFGVYNLTMRVGTARVATAGVNLVVTDPDFRQRGLMPSTI
                     RASLDAMRKNGYELSLVCNAIANYYTRFGYVSAWPEHDVTVRARDLPHDGPDFRLHRF
                     APRHRPELSQLYNTENATLTGTVVRPTFLRTKEPGDLYGYTWGVGEAVRGYLMYDVVS
                     NGRAVWHYDSAGDAGERLRVMGQIERRAGCEEVRFISLHPNSGMARAIRTLNSTADET
                     YRPTGGWLVRVINLRTLFEKLAGELSRRLACSHLAGWRGNLLIASQHEQVLLSIDRSR
                     VEMLPPEDAEHEIRGGEAIARLVIGSAEPDELVKAHNMQLSGDGMALATVLFPAQRPQ
                     MGNADL"
     gene            4689..>5609
                     /locus_tag="VFH60_02215"
     CDS             4689..>5609
                     /locus_tag="VFH60_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312624.1"
                     /translation="MQQPTGQRRNWKALIPIAALLLLVGALGAVLRFGAAGQDSPPAV
                     PRVQQAQTSIAQRSTFAGPTPTLDCTAGLAWRVVGSPNLSEAAAPGGEAAEGPAGEEG
                     EERGGSVLADVAALAQDDAWAVGSSHSGLIVETLVERWDGKEWRIAFSQVVESRGSQL
                     NSVAAVSPDYAWAVGAYQLLDRQTVPLIERWNGSEWGLVAPAPISATVGIFNGVAALN
                     ESDAWAVGYYTDERGIYRTLAQHWNGREWSTVPTPSIGQLLNTLTGVAMRATDDVWAV
                     GYRVAERNQYVPIPMHWDGKEWRMVEAPALG"
BASE COUNT          990 a         1797 c         1775 g         1047 t
ORIGIN      
        1 cgtagcgaag gatcagccgc ccggtcttga ctcttcctac tcttgcacca atgttcctcc
       61 agccgccagc atcctgtacg ttgtagactc tcgcctaccc acctgatcct tcgctacgct
      121 caggatgaca aaggggggac tctggtcccg ctatcctgcc cacgcacaac cgtgcccgat
      181 tgcggtgtca ctatctccac gtggcaaagt aatcaccgct tgccgggtgc ctacagtatg
      241 tccatgtgcc tgcccaccag cggcctgtcg aggcggaacg ggttgtcctc cgggcgtaag
      301 ctgcccgtga tggtgtccac cacgatcctg ctgccgccac cgctcgccat gatgccatgc
      361 ccgctgtagc cgcagttgag gtagaggccc tcgatagggg tagggccgag gaacggcttg
      421 tggtcgggcg tgtagctgta ctgcccggct tgcagatagt agtgggacgt ctgcctggcc
      481 cacacctcgc gccagaaggg gctgatgcgc gccaccgagt gctcgctggc ggggtcgagc
      541 aggccgaaca cgaagtccac gctgatgggt acgtcctcca gcggcgggcc gggcggcaca
      601 ccgggcgagg tgcgtagcaa gttggcccct ctcagcgcgg gacgccagtg cgcgcccgtc
      661 tcctcgtcaa tcgtcatggg tgcccagggc ggtacttccg gcacgtccgg catcacgagc
      721 ttctggcggc gcaccaggct gaggggcagc tcaaggcccg ccatgcccgc tacttctccc
      781 gcgaaaggcc ccgccgccac tactacccgc cccgtgctca cgctgcccct cgacgtatgc
      841 accgcgccga ccctcccgcc ttgcacgtca aacccggtga ctgtggtgtt ggtcgcgaaa
      901 acggcccccg tagaggcagc agcggcatag cccaacgtca ggcggcgcac gtccagccag
      961 ccgtcgccgg cgcggaagcg ggcaccaatc acgtccggcg acaggtaagg gaagcgcctg
     1021 cgcgcctcgt ccccgtcgaa ccactccacg tcggtgaggc cccaggcgtg ctgccgggcc
     1081 accagctcct tctgtatgac cacgcccgcc tccgacgtgg tgagccagag gtacccctgc
     1141 ttgcgcatgt cgaggtcgta accttcgagg cccgtcgcct gcgcgaagtt ggcgaacagg
     1201 gcgatgctct cgctcaccag ggcgatctcc tccgggttgt cgaactgcgc cctgaaagcc
     1261 cctgtggagg cgggggtggt gagggtgcaa agggcgggcc tgcgttccag cacgaggggg
     1321 cgcaggcccg cacgcgccgc gaagaacgcc gtggcggccc ccacgatgcc gcccccgata
     1381 atcacaaggt cggctgtgcg gggtaggtca tgcgtgtacg ccaccctggg ctgaattgat
     1441 tgtgctgaca gagacacggt gctgctccat caggagatat gtaatgctgc ttatgccctc
     1501 acgtcgtcgg ttagctccac gctcgacttg agcgcctggg acctgccgag tggcctgagc
     1561 gtgtaagccc caaccgaggc gggcagtacg acactgtcgt actgccccag ccgttcgctc
     1621 caaccgtcgc cttccacctg tatctcgccc tcggtgacgg tgagggcgtg gaacgtctcg
     1681 cccccggtgt ccaactcgac cggctgcgtc tccgagccga taagctccaa tgtgaaatag
     1741 tcgcagcaga ccaactgctc gcgctgcccg tcgcgtagag gcttaggggc aggggttacc
     1801 tcgcccttca tttcggggtt ggagacggcc accgattgct cgatgtgcag cttgcgaccg
     1861 ccttccaggg gcctgtccca gtcgtagatg cggtaggtga tgtcggaggt ctgttgcacc
     1921 tcgtagatga gcaggccggg gccgagggcg tgtacggtgc ccgcgtgcat caggatggtg
     1981 tcgcccttgc ccacgtcgat gtactgcgcc aactccacga ccttgccctc gcgtatcgcc
     2041 tttgcgagcg actccggcga ggtgcccgtc tcgatccctg agatgagcct ggcccccggc
     2101 tcggcgtcga gtacgtacca ggcctcggtc ttgccgaaat gttccggccc ctcaagctcg
     2161 gctgcctgct cgtcgttagg gtgcacctgg agggagagcc aatcggcgca atcgaggatt
     2221 tttatgagca gcgggaagcg gggtccggtc tgcgccatta cccgcctgcc tataagcgcc
     2281 tcgccgtact cctcggtaag gtccgcgagc gtgcggcctt catgcgggcc ggaagccact
     2341 ttgtcctgct cgtaaacaag ccaggcttcc ccgatgggtt tgtcggcgtt ggattggagg
     2401 tgctgcccgc cccagacgcg gtggtggtac tccggcacga ggcgcagggc acccagggtt
     2461 ggtttgtgtg gttgcatacc atctttatac cagagattag cttttcgccg cttcacctgc
     2521 cggccatgtc atattgtaag taggtaataa acaaccggaa ccaggatgta gctgaaacgt
     2581 tgtaggttgc agtatccacc atacgaattc gatactcact cggaggtagg agatgtgtct
     2641 gtgcaaagta gccgcctaac aactgagcag ccagcgacct gggtaagttg gaaaacaatc
     2701 cagtttctgg tgtctttgaa cttactctac cttgtcctgt cacttagtcc ctactttgtc
     2761 tataggatat ccttgcgcac gagctattct gaccctagag acttttggcc ctatggctat
     2821 gaaggactgg gtgttgccat ctggttcctg gcccgagtag ttgctgaatt gacgcctatt
     2881 ctgctgcctc tattggcaat cattacaggt acactggtga gacctttctg gaagcaacaa
     2941 ggaaaggagg caaaggtcat tatcttcctg agtattgccg ttacagggtt agttggtgtg
     3001 tcctcctggt tcttgaaccc cggtgtcacg tactggctga atgactgaca caatcgccaa
     3061 ttccaggcgt aggaacggct cggcggtggt atttaatggt actctaattg aactctaatc
     3121 agactctaat gaaaacctat tgaccacagc agccccggtt tgctactatt gaggcagaga
     3181 gcacagggta actgggtaca acgacattcc tttttccttt ctttgcgaca gtgagccggc
     3241 gatgagccgg tttcattgtt taatgccgct taataccgca caatgcgctc accccttgtg
     3301 ttatgatgta gcgttgcctt cgcgtgaagg tgccttcgca acctggcaca ccgccggtgc
     3361 ttctccagtg ccggtgggtg tcgagtcttc cctttgctgg tttggagtgc gcccttgtct
     3421 cttgagcccg taagcatcgt tgctcccggt aaaaggaagc acgccgagca gatattcgag
     3481 cttgtggcga cgcagtggcg cgatgacctg aagcggcacg cccgcgaggg ccgcatcaac
     3541 cgcagccatt acgactggaa gacctcgcgc gtcggcatcg ccaacggccg ggtcgtggcc
     3601 catttcggcg tgtacaacct cacgatgagg gtgggcaccg cgcgcgtggc gaccgccggg
     3661 gtaaacctgg tggtgaccga ccccgatttt cgccagcgcg gcctcatgcc ttccacgata
     3721 cgcgcctccc tcgacgccat gcgcaagaac ggctacgagc tgagcctcgt gtgcaacgcc
     3781 atcgccaact actacacccg cttcggctac gtatcggcct ggcccgaaca tgacgtgacg
     3841 gtgcgcgccc gcgacctccc ccacgacggc ccggactttc ggctgcacag gttcgcccct
     3901 cgccaccgcc ctgagctgtc ccagctctac aacaccgaga acgccaccct caccggcacg
     3961 gttgtgcgtc ccaccttcct gcgcaccaag gagccgggcg acctgtacgg ctacacctgg
     4021 ggcgtgggtg aggcggtacg aggctacttg atgtacgacg tggtatccaa cggccgggcg
     4081 gtgtggcact acgactcggc gggagatgct ggagagcggc tgcgggtgat gggacagatc
     4141 gagcggcgcg cagggtgcga agaggtgcgc ttcatcagcc tccaccccaa cagcggcatg
     4201 gcccgcgcca tacgcaccct caactccacc gccgacgaga cctaccgccc caccgggggc
     4261 tggctggtgc gcgtcatcaa cctgcgcacg ttgttcgaga agctggcggg cgagctatcg
     4321 cgcaggctgg cttgctcgca cctcgcgggt tggcgcggca atttgctaat cgcctcacag
     4381 cacgagcagg tgctgctttc gatagaccgc tcccgcgtgg agatgctgcc accggaggac
     4441 gccgagcacg agattcgggg tggcgaagcg attgcccggc tggtaatcgg cagcgcggag
     4501 ccggacgagt tggtgaaggc ccacaacatg cagctatccg gcgacggcat ggccctggcg
     4561 acggtgctct tcccggccca gcgcccccag atgggaaacg ccgacctgta ggggccgcgc
     4621 ttaggtgccc cattccacag cgtcattctt caaattgcat agagcaactc ccctggaaag
     4681 cagcttagat gcaacaaccc acaggccaac ggcgtaactg gaaggcgcta ataccaatcg
     4741 cggcgctgct gctgctggtc ggcgcgctgg gggccgtcct tcggttcggc gcggcggggc
     4801 aggacagccc gccggcagtc cccagggtgc aacaggcgca gacttccata gcccagcgca
     4861 gcaccttcgc cgggcctacc cccaccctcg attgcacggc gggccttgcc tggcgcgtgg
     4921 tgggcagccc caacctgtcg gaggcggccg cgccgggcgg ggaggcggca gagggccccg
     4981 ccggggagga aggcgaggag aggggcggca gcgtgctggc ggatgtcgcc gcgctggccc
     5041 aggacgacgc gtgggcagtc ggttccagcc acagcgggct aatcgtggag acgctggtgg
     5101 agcgctggga cggcaaggag tggcgcatcg ccttcagcca ggtcgtagag tcgcggggca
     5161 gccagctcaa cagcgtggcg gcggtgtcgc ccgattacgc ttgggccgta ggcgcgtacc
     5221 agctactgga ccgccagacg gtgcccctca tcgagcggtg gaacggtagc gaatggggcc
     5281 tggtagcgcc cgcgcccatc agcgccacag tcggcatctt caacggcgtg gccgcgctca
     5341 acgagagcga cgcctgggcg gtcggctact acaccgacga gcgcggcatc taccgcacgc
     5401 tggcgcaaca ctggaacggc cgcgaatgga gcacggtgcc cacccccagc atcgggcagc
     5461 tcctgaacac cctaaccgga gttgccatgc gcgctacaga cgacgtgtgg gcggtgggat
     5521 accgggtcgc ggagaggaac cagtacgtcc ctataccgat gcactgggat ggcaaagagt
     5581 ggcgcatggt agaggcccct gccctcgga
//
LOCUS       DASRWZ010000152         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_423596, whole genome shotgun sequence.
ACCESSION   DASRWZ010000152 DASRWZ010000000
VERSION     DASRWZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423596"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VFH60_02220"
     CDS             <1..727
                     /locus_tag="VFH60_02220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6312625.1"
                     /translation="RCLEAISGHTGSLLEIGCGAGRYTRAFLHYRPELEVHGCDISQV
                     ALEEARQADRTGKIDYVLGDALDLPYPNNSFDIVVLFDVFEHVTDVGKAASEVARVLK
                     PGGVFHCFVPCEGNKRTIFALLRHSKRVPIHMWKRVHIGHIQVLTTKQMKQILLRREL
                     RVTDATFSFHILGQIHDVFDYWRRDMLSRDDLAKWQRVLVRAISRAVFIPTWRLAYFE
                     DTLLKRNPSAIGVHLTARNEKAA"
     gene            803..>1697
                     /locus_tag="VFH60_02225"
     CDS             803..>1697
                     /locus_tag="VFH60_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312626.1"
                     /translation="MRPHFSFQSLLPWAAALAVLLGVWLLPLPATMFGQEQVSAERMP
                     ETRGTANAAIGDFFKARQAYYDFITSHDMAVISSSLHSALAEARRSPQSTGVLAEVLH
                     WGIQVRDYNAVLAGYAEAGDKVFPVLRYYDDELMKYTRSLSPPTGEVRALTFPLADHQ
                     RLYPPPIGDLMPDPPWIKEDVIKSQLETINGQIRAIQAAVTGGNAPSNMPQVLDGLAR
                     SVGDIWESGRSVEQVGLQHTKYIDHLQTYETKFQQYLDAHGPNSISSTRRMLAYGANM
                     AVGLVLAGGVALLLMPRKKPVG"
BASE COUNT          320 a          570 c          517 g          290 t
ORIGIN      
        1 acgatgcctc gaggcgataa gcggtcacac cggcagcctg ctggagattg gctgcggggc
       61 cgggcgctac acacgcgcgt tcctgcacta ccgccccgag cttgaggtgc acggctgcga
      121 catctcacag gtggccctgg aagaagcccg ccaggccgac cgcacgggca agatcgacta
      181 cgtgctgggc gacgccctcg acctgcccta tcccaacaac tccttcgata tcgtggtgct
      241 gttcgacgtg ttcgagcacg tcaccgacgt tggcaaggcc gcctccgagg tggccagggt
      301 gctcaagccg ggcggggtgt tccactgctt cgtgccgtgc gagggcaaca agcgcaccat
      361 attcgccctg ctccggcaca gcaagcgcgt gcccatccac atgtggaagc gcgtgcatat
      421 cggccacatc caggtgctga cgaccaagca gatgaagcaa atactcttga ggcgcgagct
      481 gcgcgtcacg gacgccactt tctcgttcca catcctgggg cagatacacg acgtgttcga
      541 ctactggcgg cgagacatgc tttcccgcga cgacctggcg aagtggcagc gtgtgctggt
      601 aagagccatc agccgcgccg tgttcatacc cacctggcga ctcgcctact tcgaggacac
      661 cctgctcaag cgcaacccct cggctatcgg cgtgcacctg accgcccgca acgaaaaagc
      721 cgcctgagtt ggccgtcgca accggccggg ggtggcggag ttgtactttc aacatgacct
      781 tgctagctga ccgttaccca acgtgagacc tcacttctcc ttccagtcgc tactcccctg
      841 ggctgccgcg ctcgcggtgc tgctcggtgt gtggctcctg ccgctgcccg cgacgatgtt
      901 cgggcaggag caggtatcgg cggagcgcat gccggagacg cgcggcacgg ccaatgccgc
      961 gattggcgac ttcttcaagg cccgccaggc ttactacgat ttcatcacga gccacgacat
     1021 ggcggtgatt tcaagcagcc tgcacagcgc gctggcggaa gccaggcgaa gcccgcaaag
     1081 cactggcgtt ttggccgaag tgctccactg gggcatccag gtgcgcgatt acaacgccgt
     1141 gctggcgggc tacgccgaag cgggcgacaa ggtgttccct gtcctgcgct actacgacga
     1201 cgagctgatg aagtacacgc gctcgctcag ccctcccacc ggggaagtgc gcgccctcac
     1261 cttccccctg gccgaccacc agcgcctcta cccaccacct attggcgacc tcatgcccga
     1321 cccgccctgg atcaaggaag atgtcataaa atcgcagctt gaaacgatca acggccagat
     1381 tcgcgcgata caggccgccg tgaccggcgg caacgctccg agcaacatgc cccaggtgct
     1441 ggacggtctt gccaggagcg tgggcgacat ctgggagagc gggcgctcgg tcgaacaggt
     1501 gggtttgcag cacaccaagt acattgacca cctccagacc tacgagacca agttccagca
     1561 gtacctcgac gcccacggtc cgaacagtat ctcttccacc cgccgcatgt tggcctatgg
     1621 cgcgaacatg gcggttgggc tggtgctagc gggcggggtg gcgctcctgc tgatgccccg
     1681 caagaagccg gtcggaa
//
LOCUS       DASRWZ010000153         4675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_561210, whole genome shotgun sequence.
ACCESSION   DASRWZ010000153 DASRWZ010000000
VERSION     DASRWZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561210"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFH60_02230"
     CDS             complement(<1..534)
                     /locus_tag="VFH60_02230"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6312627.1"
                     /translation="MNFKVVIDVLKDTFKDFGEDKVPRLAAALAYYTIFSIGPLLLIA
                     IAVASLVFEKTDVEGQMLDTIQGLLGKEGRDTIESALEAGNKGGGNIVAASIGIATLL
                     MGASGVFGQLKDALNTIWEVKPKEGQGVLGILRERFLSFTMVLGTGFLLLVSLVVSAG
                     IAALGTFLKSVLPGGDWV"
     gene            complement(666..2015)
                     /locus_tag="VFH60_02235"
     CDS             complement(666..2015)
                     /locus_tag="VFH60_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6312628.1"
                     /translation="MLSTADSTIRELEDECVRHLQALIRLDTSNPPGNEVLAVEYIRG
                     QLAEAGVSAEVVESEPGRANLRAVLKGDGSERPLLLMSHTDVVPVEPEFWTHQAPFSG
                     EIDGGYVWGRGAVDMKQWVAWHLTIFLDLARRAVPLKRDIVLLCTADEEDGSYKGMRW
                     IAEHMPEWLDGEYGLSEGGGGEMQVRGRSFFPCRVAEKGACRFRLRAHGSPGHASRPH
                     SDNALVKLGTALGRLGAFQMPLQPSDIVRGMLERIFEDEPRAENKVGDFLNEYTVEMA
                     LQDSPFSPAARLTLNAQLRNTAAPTILHAAGSRINVIPSTAEADVDGRVLPGVSMEDF
                     ARQVVEIVGPDVEVEVYEYWPGSASSFDTDLFRIMQDVTKELTGATLVPFMATGASDA
                     RFAEPLGVDVYGFGLMRDEPGAAPSALMHAHDERISIDNVYLGLHALHETVCRIATL"
     gene            2158..2535
                     /locus_tag="VFH60_02240"
     CDS             2158..2535
                     /locus_tag="VFH60_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET6312629.1"
                     /translation="MPKTVTATDAKNKFGSLVNWVVENSDEVIVENHGSPAVVLISVS
                     EYEGYKAFREVERRKRLLAELTELRERVRERNPDITTDEQAFEIADEIVRDAIDSLVA
                     KGKIRFAADNSGDNSADNSSGNS"
     gene            2535..2975
                     /locus_tag="VFH60_02245"
     CDS             2535..2975
                     /locus_tag="VFH60_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HET6312630.1"
                     /translation="MLRVLLDANVYISYLLAPDAPGTIQQLMSAALEGRYTLLLPREL
                     LAEMSETLSRKQALSRRTNPQERQKLLSRFMEIAEKIDTIREEIPTVTRDRKDDYLLA
                     YAVIGQVDYLVTGDMDLLVLDHVGPVQIVRPADFLAVLSRQSPS"
     gene            complement(2986..4395)
                     /locus_tag="VFH60_02250"
     CDS             complement(2986..4395)
                     /locus_tag="VFH60_02250"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6312631.1"
                     /translation="MSSAGAVISDLEGISDPDFRMLGEQVVEAMARLSVPGVAVGLIH
                     GDEEHTAGFGVTSIANPLPVDADTLFQIGSTTKTVTGTIAMRLVEQGKLDLDTPVRAY
                     LPELRLSDEDVAGRVTLKHLFTHTGGWVGDYFDDFGMGEDALSRMVAKLAGLPQLTPL
                     GEVWSYNNAGFYIAGRIIEVVTGQPYEQVARELVLGPLGMHMSFFFPSEAITYRVAIG
                     HNNPYLDGKFLPRIARPYALPRTSSPVGNIISTAKDQLRYARFHMGDGTAEDGTRLLS
                     SESMLLMQTPHVAAADGQSFGITWFIREIEGTRVLRHGGATNGQMSAFVFVPSHRFAI
                     TVLANADRGRELHQEVTTWALEHYLGLKPEEPGLMERDEHQLASYAGLYTGPMTDLDL
                     QLREGGLVLQDVPKVGLPGAELQPPGPPMRLAFCGEDLVTVLDEPMKGAHGEFLRGPE
                     GEIRWLHISGRVFGRAVGE"
     gene            4547..4620
                     /locus_tag="VFH60_02255"
     tRNA            4547..4620
                     /locus_tag="VFH60_02255"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4581..4583,aa:Val,seq:gac)
BASE COUNT          915 a         1395 c         1478 g          887 t
ORIGIN      
        1 tacccagtcg ccgccgggca gcacgctctt caggaacgta ccgagggcag cgatacccgc
       61 gctcaccaca agggaaacga gcagcaagaa accggtgccg agcaccatcg tgaacgacag
      121 gaacctttcg cgcaggatgc cgaggacgcc ctgcccttcc ttcggcttga cctcccagat
      181 ggtgttgagc gcgtccttca attgcccgaa cacgcctgac gcgcccatta gcagggtcgc
      241 aatacctata gaagcagcaa caatattacc gccgcctttg ttgccggctt ccagggcaga
      301 ctcgatggta tcacgaccct ctttaccaag gaggccctgt atcgtatcca gcatctggcc
      361 ttctacgtct gtcttttcga agacgaggct ggcaacggca atcgcaatca gcagaagggg
      421 gccgatggag aagatggtgt agtaggcgag ggccgccgcc agccggggca ccttgtcctc
      481 gccaaagtcc ttgaaggtgt ccttcaggac atcaattacg actttgaagt tcatgcatag
      541 actccttgtg caatcctcac aaccggctat gcaagcgccg taccactggc gattttggat
      601 tttagacttt ggattttgga tttgcttgac gttcttctga atccaaaatc taaaatccaa
      661 aatcgtcaga gtgttgctat ccgacacacg gtttcgtgga gggcgtgcag gccgaggtag
      721 acgttgtcaa tggagatgcg ttcgtcgtgg gcgtgcatga gggcggaggg ggcggcgccg
      781 ggttcgtcgc gcatgaggcc gaagccgtac acgtcaacgc cgaggggttc ggcgaagcgg
      841 gcgtcactcg cgccggtcgc catgaagggc accagggtgg ccccggtgag ttctttggtg
      901 acgtcctgca ttatgcggaa caggtcggtg tcgaagctgc tggccgagcc gggccagtac
      961 tcgtacacct ccacctccac gtcggggccg actatctcaa cgacctgccg ggcgaagtcc
     1021 tccatggaga cgccggggag cactctgccg tccacgtcgg cctcggcggt ggaggggatg
     1081 acgttgatgc gactgcccgc ggcgtgcagg atggtgggcg cggcggtgtt gcgtagctgg
     1141 gcgttgaggg taaggcgagc ggcgggggag aagggcgagt cttgcagcgc catttccacg
     1201 gtgtattcgt tcaggaagtc gcccaccttg ttttcggcgc gaggctcatc ctcgaatatg
     1261 cgctccagca ttccgcgcac tatatcgctg ggttgtagcg gcatctggaa cgctcccagc
     1321 ctgcccagcg cggtgccaag cttgacaagg gcgttgtcgc tgtgcgggcg ggaggcgtgg
     1381 ccgggcgagc cgtgggcgcg caggcggaag cggcaggccc ccttttcggc cacgcgacac
     1441 gggaagaagg agcggccccg cacctgcatc tcgccgccac ccccttcgct caggccgtac
     1501 tcgccatcca gccactccgg catgtgctcg gctatccagc gcatgccctt gtacgagccg
     1561 tcttcctcgt cggcggtgca cagcagcaca atatcgcgct tgaggggcac agcccggcgg
     1621 gcgaggtcga ggaagatagt caggtgccag gcgacccact gtttcatatc gaccgcgccc
     1681 cggccccaca cgtagccgcc gtctatctcg ccggagaacg gggcctggtg ggtccagaac
     1741 tcaggctcga ccggcaccac gtcggtgtgg gacatcagca gcaggggccg ctcggaccca
     1801 tcgcccttga gcacggcccg caggttggcc cgccccggct cggactccac cacctccgcg
     1861 ctcacccctg cctcggccaa ctgtcccctg atgtactcca cggcgaggac ttcgttgcct
     1921 ggagggttgc tggtgtcgag gcggatgagg gcttgcaggt gccgcacgca ctcgtcctcc
     1981 agttccctga tcgtactgtc agcggtagaa agcatctcgc gctccctttt tcagccatta
     2041 tacgatggct agacaaatcg tatgataacg ctacttgaca gctaatattt tgtctaatat
     2101 attgtacaaa ataaagataa ggacagccgg agtagaaagc ctagactgga gcctgccgtg
     2161 cctaaaaccg ttacggctac cgacgccaag aacaagttcg gttcgctggt caactgggta
     2221 gtagagaaca gtgacgaggt tatagtggaa aaccacggtt cgcctgccgt ggtgcttatc
     2281 tcggtgagcg agtacgaggg ctacaaagcg ttccgggagg tagagcggcg aaagaggcta
     2341 cttgcggagc ttaccgagtt gcgcgaacgg gtgagagagc gcaacccgga cattacgaca
     2401 gatgaacaag cattcgagat tgccgacgag atcgtgcgcg atgccatcga cagcctcgta
     2461 gctaaaggca aaatccgctt tgcggccgac aactccggag acaactccgc agacaactcg
     2521 tctggcaact cctgatgttg agggtgctgc ttgatgctaa cgtatacatc agctatttgt
     2581 tggcaccgga tgcccccggc accatacagc agttgatgag cgcggcactg gaaggcagat
     2641 atacgctgct actccccaga gagcttctcg cggagatgtc ggaaaccctg tcgaggaagc
     2701 aagcgctttc ccgcaggacc aacccgcaag agcggcagaa gttgctttcg cgcttcatgg
     2761 aaatagcgga gaaaatagat accatcaggg aagaaatacc gactgtcacg cgagaccgca
     2821 aggacgatta cctgctggcc tacgcagtca taggccaggt tgattacctg gtcacgggcg
     2881 acatggacct gctcgtgcta gaccacgtcg gcccggtcca gatagtgcgc cccgctgact
     2941 tcctggcggt cctcagccgg caatcccctt cttgacctaa gctgcttatt ccccgaccgc
     3001 gcggccaaag accctgcccg agatgtgcag ccagcgtatc tccccttccg gcccacgcag
     3061 gaactcgccg tgcgcccctt tcatcggctc atcgagcacc gtaaccaggt cttcgccgca
     3121 gaaagcgagg cgcatgggag gtccaggagg ttgcagctca gcgcctggca agcccacctt
     3181 cggaacgtcc tgcaagacca ggccgccttc gcggagttgc aggtcgaggt cggtcatggg
     3241 gccggtgtac agcccggcgt aggaggcgag ttggtgctcg tcgcgctcca tcaagcccgg
     3301 ctcttccggc ttgaggccga gatagtgctc cagcgcccag gtggtgactt cctggtgcaa
     3361 ctcgcggccg cggtcggcat tggctagcac ggtgatcgca aagcggtggg acggcacgaa
     3421 cacgaaggcc gacatctggc cgttggtggc cccgccgtgc cgcaagacac gcgtgccctc
     3481 tatctcgcgt atgaaccatg tgatgccgaa ggattgcccg tcggcggcgg ccacgtgggg
     3541 cgtttgcatc aggagcatcg actcggagga gagcaggcgc gtgccgtcct cggcggtgcc
     3601 gtcgcccatg tggaagcggg cgtaccgtag ctggtccttc gcggtcgaga tgatgttacc
     3661 caccgggctg gaggtgcggg gtagagcgta gggccgggcg atcctcggga ggaacttgcc
     3721 atcgaggtaa gggttgttgt gcccgatagc cacgcggtac gtgatcgcct cggaggggaa
     3781 gaagaacgac atgtgcatgc ccagcgggcc tagcacaagc tcgcgggcca cctgctcgta
     3841 aggctgcccc gtgaccacct cgataatgcg gcccgctatg tagaagcctg cgttgttgta
     3901 cgaccagacc tcgcccagcg gggtaagctg cggcaaaccg gccagtttcg ccaccatacg
     3961 cgaaagcgcg tcctcgccca tgccgaagtc gtcaaaatag tcgcccaccc agccgccggt
     4021 gtgggtgaac aggtgcttga gagtgaccct gcccgccacg tcctcatctg acaggcggag
     4081 ttcgggcagg taggcgcgca cgggcgtgtc gaggtccagc ttgccctgtt cgaccaggcg
     4141 catggcgatg gtgccggtga cggtcttggt ggtggagcct atctggaaga gcgtgtcggc
     4201 atcgacgggc agggggttag cgatgctggt gacgccgaag cccgccgtgt gctcctcgtc
     4261 gccgtggatg aggcccacgg ccactcccgg cacgctcagc cgcgccatag cctccactac
     4321 ctgctcgcct agcatgcgaa aatcggggtc gcttataccc tcaaggtccg aaatgaccgc
     4381 ccccgcagat gacatgtgcc tacctccgtg tggtgtgaat gtgtagggat agtataacag
     4441 ggacgggcga ggtgatcagg actgaggact gaggactgag tggcgcttga gcgcgtgcgg
     4501 cgttcgcttg acatgattgt gccgagtagc tataattaaa tgcctcgggc ggttagctca
     4561 gttggttaga gcgctacgtt gacatcgtag aggtcattgg ttcgagtcca ttaccgccca
     4621 ctatagttta cgcacgacag aagggcctgg cagcagttgc caggccctct cttct
//
LOCUS       DASRWZ010000154         1588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_561255, whole genome shotgun sequence.
ACCESSION   DASRWZ010000154 DASRWZ010000000
VERSION     DASRWZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561255"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1452
                     /locus_tag="VFH60_02260"
     CDS             181..1452
                     /locus_tag="VFH60_02260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6312632.1"
                     /translation="MPYRILTIAPTSFFSDYGAHVRILEETVHLQRRGHAVLLCTYHI
                     GDNVPGVNIRRSVDVPWKRGVMVGSSRHKLYFDVALAATVQRAAAAFKPDVVHAHIHE
                     GALLGWTVRQVRGVPLVFDYQGSMTAEMLDHRFIREKSPLLRPLKWLEARIDRSADAI
                     ITSSHNAETGLRARTGDYSDRISTVADAVNTQTFSPPATGMERAACEALKRSLGIPAG
                     RRVVVYLGLLAPYQGTDLLLEAAAIMVNEWGMRDVQFLVMGFPGVDSYRGQADRLGLN
                     GCVSFPGRISYADAPRYLAVGDVAVAPKLSETEGAGKIGNYMAMGLPVVAFDTPVSRE
                     YLGPLGVYAERGNSRSLAEKLRQALDDPGRYREVGARLRQECVARLSWDAAIDRIEAV
                     YERAGARRAGKRAPDTAMLRISDTPQEALGD"
BASE COUNT          299 a          487 c          516 g          286 t
ORIGIN      
        1 agggcgaggc atgcctcgcc cctaccccgc tattctggac acatactttg cctgccctaa
       61 ctcctgaccc ccgcccctcg gacctcgctc ctcgaacctt gctcctgcac aatttgacac
      121 cccaacaagg gcggcaatat aatggcaggc agaggaaaga gctagattag cgagagatta
      181 ttgccctacc gcatattaac aatcgccccc acttcgttct tctccgatta tggcgcgcac
      241 gtccgcatac tggaggagac cgtacatctc cagcggcgcg ggcatgccgt cctactctgc
      301 acctaccata taggcgacaa cgtgccgggc gtcaatatca ggcgctctgt agacgtgccc
      361 tggaagcggg gcgtgatggt cgggtcgtcg cgccacaagc tctactttga cgtggcgctg
      421 gcggcgacgg tgcagagggc ggcggcagcc ttcaagcctg acgtggtgca tgcccacata
      481 cacgagggcg cgctgttggg gtggaccgtg cggcaggtac gcggtgtgcc cctcgtcttc
      541 gactaccagg ggagcatgac cgccgagatg ctcgaccacc gcttcatccg cgaaaagtcc
      601 cctcttttgc gccctctcaa atggttggag gcgcgcattg ataggtcagc cgatgctatt
      661 atcacctcat ctcacaatgc cgaaacaggg ctgagagcgc gcaccggcga ctactcagac
      721 cgcatctcga cggtagccga cgcggtaaac acccagactt tctccccacc tgccaccggc
      781 atggaacgcg cggcatgcga ggcgctcaag cgttcgctcg gtatacccgc cgggcggcgc
      841 gtggtggtct acctggggct gctcgcgccg taccagggca ccgacctgct gcttgaagcc
      901 gccgccatca tggtaaacga gtggggtatg cgcgacgtgc agttcctggt gatgggcttc
      961 cccggcgtgg acagctatcg cggccaggcg gacaggctcg gcctcaatgg gtgcgtctct
     1021 ttccctggcc gcatatctta cgcggatgcc ccccgttacc tggcggtcgg agatgtagcg
     1081 gtagcgccca agctgtcgga gacggaagga gcaggaaaga taggcaacta catggcgatg
     1141 gggctgccgg tggtggcctt cgatacgccc gtgagccgtg agtacctggg gccgctgggg
     1201 gtgtacgctg agcggggcaa ctcgcgctcg cttgccgaga agctgcggca ggcgctcgat
     1261 gaccctgggc gctacaggga agtgggcgcg aggctccggc aggagtgcgt cgcaaggctg
     1321 tcgtgggatg ccgccattga ccgtatagag gcagtgtacg agagggccgg ggcgcgcaga
     1381 gcaggcaaga gagcgcccga taccgccatg ttgcgcataa gcgatacccc ccaggaggcg
     1441 ctgggtgatt gaccaggcca gggcgggtgg tccggcaagg gtcgccgtcc aactgtaccg
     1501 gggcaacagg ttcaagaaac tggtcaagcc tttcgcggtg aaggctatca gcgcgttctg
     1561 gctgcgattt tacaaagcga agtacggg
//
LOCUS       DASRWZ010000155        16407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_423898, whole genome shotgun sequence.
ACCESSION   DASRWZ010000155 DASRWZ010000000
VERSION     DASRWZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 16407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16407
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423898"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..777)
                     /locus_tag="VFH60_02265"
     CDS             complement(73..777)
                     /locus_tag="VFH60_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6312633.1"
                     /translation="MSSMLTVDAIHTFIGQYHILEGVSLDVPRGSITALLGRNGAGKT
                     TTLKSVIGLLSPRMGKIVFDGEEIQGLTPYAIAARGVGYVPEHRAVFRDLTVEENLKI
                     AERKRGDLARHSDLIFDLFPDLRRFYKLPGGQLSGGQQQMLSIARALVADNRLLLIDE
                     PSEGLAPIIIEAIVHALRRLSEHITVLLVEQNFRMASQLADRYYILDTGHTAHEGQMS
                     DLVADQELITRYLGAA"
     gene            complement(770..1591)
                     /locus_tag="VFH60_02270"
     CDS             complement(770..1591)
                     /locus_tag="VFH60_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6312634.1"
                     /translation="MDTYIIEARDLSKHFGGLRAVDGVNLQVREGSLHSIIGPNGAGK
                     TTLFNLISGNLKPTRGRVFLRGRDITGTPLHRMAHMGIGRSFQITNIFPNLTVLENAR
                     LAAQAMGRDSLNLWSMATRFRQYEEAAMTALKQVGLSERAGQAASILPHGDKRKLELA
                     ILLAAGPQVLLLDEPTAGMASEQVPMLLDIIRRIREDTGKTILLVEHNMGIVMNVSDR
                     ITVMHQGSVLAEGTPDEIRTSRAVQEAYLGESYTEPQLPGVAPLTAATSEGTDNV"
     gene            complement(1683..3494)
                     /locus_tag="VFH60_02275"
     CDS             complement(1683..3494)
                     /locus_tag="VFH60_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6312635.1"
                     /translation="MSKNAKRWSSILASLMLVLMALPIGGTNTYAQAGSQTFPETGKT
                     VKGRFLEYWQQNGGLAQQGFPITDEMSERSDTDGKTYQTQYFERAVFELHPENQKPYD
                     VLLSLLGVFQYKAIYGAAGPAGQKVSTAAGARRFAETGMTVGGKFLDYWNKNGGLAQQ
                     GYPISQEFTEVSALNGKPYTVQYFERAVFELHPENQAPNDVLLSQLGTFRLKQKQAAA
                     KGPVNVGLLTDRSGSLAIYGPMLEQGFALGLEYATGGTNKVYGRDIKVTTKDTASAVD
                     TGTSLAREAIEKDKAQVLVGVPSSGVALAVSSVANDNKVVYLAGPAASPDITGKNFNR
                     YTFRAGRSSVHDALTMGTALTGMGKKFIQIAPDYAFGRGSAAAFYNVIKAKGGTFVVN
                     DNTTDFGTVFAPQDTTDFTPYLNQVLDSGAEVVVVTWAGTGFVPLFQQMQQLGIFDSM
                     VVATGMGDNQTLAKGYADAIGSVGVSVYHYSLFDTAINKWLVDKHKAKYNTPPDLFTE
                     SGFIAAQMLVRSITMNNGEADAEGLIKALEGMTFDGPKGAYTIRAEDHDLLQPMTLVK
                     LKNTTDKDYRFFELVKAFKPEETAPPCTVPAAANRCK"
     gene            complement(3683..5554)
                     /locus_tag="VFH60_02280"
     CDS             complement(3683..5554)
                     /locus_tag="VFH60_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6312636.1"
                     /translation="MDADNLRVHYIEQGEGEPIVLVHGNWGTSSWWEPVLERLPDGYR
                     GIAYDLRGRGKTEGPDNDYTMPEMAADLRAFLDALGLDRVHLVGHSLGSAVAMQFLLE
                     WPERVLTFVAVSPAWVDGMPQAYNVPAGQQAIKADRAMFATALKALAPGVADDEYWQR
                     LVVEGHEQRIEAALRNLPALVAWKPGDKLRDAGVPALVIAGDRDPLTGGANATRAAEA
                     LGAGHVILQGTGHSPNIEAPDEFVRLLLEHLSGGRAAESTTTQKELSMANGTLLPGIT
                     EATVDTGRLKMHVLVSGPQDGDPVVLVHGNMSSGPFWEETMLTLAAEGYRVLAPDLRG
                     YGDTEAVPVDGTRGMRDWSDDLKALVTALDLGRFHLLGWSMGGGVAMQYAIDNGADLL
                     SLTLESPVSPYGFGGTKDAQGTPNHDDFAGSGGGTANPEYVRLVKEGDRGGESPFSAR
                     SVLNNFYFKPPFRVAPEREEAFVTSVLKTRIGDEFYPGGMTDSPNWPNVAPGTGGFNN
                     AMSPRYLNLGAFANIEHRTPVLWVRGADDQIVSDTSLFDFGFLGQIGAVPGWPGAEVC
                     PPQPMIAQTRAVLDAYKANGGDYWELTITDSGHSPHIEKPEEYNAALLKHLRLAAGR"
     gene            complement(5584..7344)
                     /locus_tag="VFH60_02285"
     CDS             complement(5584..7344)
                     /locus_tag="VFH60_02285"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET6312637.1"
                     /translation="MNHFLKAHKPSIPVAIPGAACILALLLLAGALSSAGRHGTAVAA
                     PLTDSPTFGVVQAGSFITETYGDRDYRLYIPEGYQAGTPLPLLVVLHGCGQDPTSMSV
                     DTHFNVYADQFQFLALYPRQPQSANGTRCWNWFEPAHQQRGAGEPALIAGMTAQVQSR
                     YSVDSTRIFATGLSAGGAMSIIMGATYPDVYAAIGVSAGIEYGCATDTASGLVAMASG
                     GCADPVESGNKAYAAMGQYKRVVPVIVFHGSADAVVVVKNAHNILTQWAQTDDLAADG
                     ADNNDIDDVPEGTSPGQVPGGHTYTRYTYNDSRDGRTVLEKYIVDAMPHAWSGGTLDP
                     ANGGDRNVDPLGPDASLIMWQFFQANPMPGNEPMPTATAIPCAVQFPDVPQGSAFYAY
                     VRCLACQGILGGFADGTFKPGSNVTRGQLSKIVANAAGFNEQVGGQTFEDVPPGSTYH
                     PFIERMAARHIIGGYPCGGPGEACGEGDRPYFRPNASASRGQIAKIVSDAAGYAESPG
                     AQRFEDVAPGSAFYEGVQRLASRGHVSGYECGGASEPCGEGNRPYFRPGDDASRGQTA
                     KIVANTFFPGCETSTQGMPH"
     gene            complement(7348..8157)
                     /locus_tag="VFH60_02290"
     CDS             complement(7348..8157)
                     /locus_tag="VFH60_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6312638.1"
                     /translation="MPRVSVNDINLYYEFRGRAEGPTVVFVNGLLTDTASWNKHLPFF
                     EEQYRCLVYDCRGQGQSDKPDHVYETALHIADLAALVDAVGVERAHFVGLSNGGVAVL
                     AYAANRSERVISAVASGAYPYVDTILKVKLTSWVRAMEAGGSPLRFDVATPWVWGGRF
                     LDENYEGLLYFREKGVNMPIEWAMNLIKGAMLHDISDRLSHIQAPTLVMVGEEDVLTP
                     PALSRYIAERVPRSELHVMQGRGHASALEDIPQFCQVVLDFFHRVEDAQGG"
     gene            complement(8233..9771)
                     /locus_tag="VFH60_02295"
     CDS             complement(8233..9771)
                     /locus_tag="VFH60_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET6312639.1"
                     /translation="MYIGDWLARREMLTPGKVALIDAQREFQPITYGEWNRTANRTAN
                     WLRALGVGQGTLVAVLSMNCVEYLDVWFACGKLGAIMQTLNWRLTGRELVALLQDATP
                     QVLIYGPDFVAQARAIQSEVPGIEHYAALDPRQVASPGDRLFVEREACPDSPPPPVEL
                     TWDSPWALAYTGGTTGLPKGAILTHGNVTWNSVNTVMSWGLSPDDRTFLNMPLFHTGG
                     LNVLTAPLVHIGGTSIVCRGFDVDQVFDLVEHMGVTIYFGVPTMFIAQQAHPRWATAD
                     FSRLKWVISGGAPCPPPVFEKWWARGVDFKMGYGLTESGPNTFWMTSENARRKPGSVG
                     VPLFHVDVRVVRPDGIECGPDEVGELHIRGPQVVPGYWNRPEESAKTIVDGWLHTGDL
                     ARRDEEGFYYIVGRLKDVIISGGENIYPAEIEAVISGHPSVASVALIGVTDEHWGEVG
                     RAVVVLNDGCMPDGDGIIAYCRERLARYKIPKSVVFADTLPVTGAGKIDKLALKERYG
                     DFRF"
     gene            complement(9920..10828)
                     /locus_tag="VFH60_02300"
     CDS             complement(9920..10828)
                     /locus_tag="VFH60_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6312640.1"
                     /translation="MGTNFRLDGRVAVMTGGANGIGRQTAFTFAASGAAVAVWDMAED
                     QGQALATEIRDSGGRAAFYKVNVTSASEVEAAVAVWDMAEEQGQALVTEIRDAGGKAA
                     FYKVNVTSTSEVEAAVAAVVEQFGKVDILVNNAGITRDAQLVKVKDGEVTGKMSEADF
                     DLVVSVNLKGVFNCTQAVAPHMITRGYGRIVSASSVVGIYGNFGQTNYVATKAGVIGM
                     TKVWARELGKRGITVNAVAPGFIGTDMVRAMPEQVLSNMVSHTPVGRLGEPEDIAYAY
                     LFLASEEASFINGAVLSVDGGLVLGT"
     gene            complement(10807..11832)
                     /locus_tag="VFH60_02305"
     CDS             complement(10807..11832)
                     /locus_tag="VFH60_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HET6312641.1"
                     /translation="MSRSSAGIVAAGAYIPQTYITAAEIAEHTGIPEEVLVSKFGLRR
                     KPIPGPDDHPNAMAVKAAQDALSRADVRPEEIDVVMSTTEEYKEYPLWTTGIKLAYDI
                     GANRAWAVDVQLRCGTTVAALRMAKAMMLYEPNVNTVLIAGGYRNGDLVDYANPRSRF
                     MINLSCGAGALILRKNHPGNHLLGSAIITDGSFSLDVVIPAGGTMEPLTAEGLSAGRY
                     YLDVPDPEGMKQRLDTLSMQNFLKVIDMALEESGCTRGDIDYLNILHMKRSAHDYVLR
                     ELGLRDNQTFYLSDFGHMGQQDGIISIVQGLESGRLKDGDLMVIVAAGIGYTWGASVV
                     RWGPTSD"
     gene            complement(12010..15342)
                     /locus_tag="VFH60_02310"
     CDS             complement(12010..15342)
                     /locus_tag="VFH60_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET6312642.1"
                     /translation="MSEPMYPAPTRREDLLLRTKLAPPQVHSRVLPRPALASLLREAL
                     QYRLTMLHAGTGYSKTTALAALDNGEIPLFWYTLGDAESDPQRFLSYLISAFRVRMPG
                     MSDLPLVVLRERGSEGSKAAWVQALDALINALAEGPGEPALLVLDDYHFVGNSPEITA
                     LVERFLEYLPSNLHVIVSTRYPLAFPKLVTWRARGEVLEIGRKALAFGHEEVAALFRD
                     VYRLELAPEELHALVEKTEGWPIALQLVWQGLRGGTTRSTSQLLEQGSTSLSALFDYL
                     ASDVLGAQPTDVASFLRDTAILRELTPAACDAVTGCSDSGAMLDRLLEMDLFMVALGD
                     PSERHYRYHHLFHDFLRQQSQADAEDTLKRHHLAAEFFAGAGDYEEAIYHWLAAGDLS
                     QVAGAIERAGETALRAGWLDTVATWIDPLPPDVLAAHPLLQAFLGDIYRMRSRFDEAL
                     AWYAQAERVWRARDDRAGTSRALRGQALLYLDTVRPAQAESLLEEALRLIEGLPDRES
                     RARLLELLAENKLNMGKPSEAEELRSEARSLREEGPGEDTLSVRVKLRTGRLDEAQRT
                     LEQWAEAERREVELGQVHPPRSHRETLLILSLIHSFRGQPERAIELAQEAIDVARRLD
                     SPFIEAVGQTRLGHALQIRRDLTSPAPNAQHVEEAIRGYGAAVALGDKLAVRRMRGEA
                     MWGLTRTYGFSGDLASAERSAAEGAEIGRWAGDAWIVALIELTLGASYVLGGQPQRAS
                     DLLSQVLVSFWGCGDSFGRAATRLWLALAYHDLGQAELCNVSIDEALALCEAHGYDFL
                     FTAPSLLGPPDIRRAVPLLVRARGNRRNAAYISRLLSQIGLPDAQVHPGYQLRVYLLG
                     TFRVFRGDVEVEPREWQRDKARQLFQLLLTQRGRWLQRDEVVERLWPNLSPEAALRDF
                     KVALNALNKAIEPGHASDAAFAYIVRDGVSYRLRPEADLWLDTDEFERRCQAGSRLAD
                     ARESEEAVANLRLALDLYAGNYLPDALYEDWASAERERLLSIFLRAADRLAGLLVEGG
                     QYDEALEVCARILSYDACWEGAYRWMMLAYAHRGDRTLALRAYQRCRTTMSAELGVAP
                     STATTALYNRLLKAEDLPVTVL"
     gene            16302..>16407
                     /locus_tag="VFH60_02315"
     CDS             16302..>16407
                     /locus_tag="VFH60_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET6312643.1"
                     /translation="MDMGLKGTVAVVIASSQGLGKATALGLAEEGANLV"
BASE COUNT         2830 a         5211 c         5258 g         3108 t
ORIGIN      
        1 ttggatttta gattttggat ttagaagaac tcccggcact ccaaaatcta aaatccaaaa
       61 tctaaaatcg tctcacgctg ctcccaaata ccgggtaata agctcctggt ccgctacgag
      121 gtcggacatc tggccttcgt gggcggtgtg gccggtgtcg aggatgtagt agcggtcggc
      181 cagttgggag gccatgcgga agttctgctc gaccagcagc acggtgatat gctccgacag
      241 gcggcgcaga gcgtgcacga tagcctctat gatgataggg gccaggccct cgctcggctc
      301 gtctatgagg aggagccggt tgtcggccac cagggcgcgg gcgatggaga gcatctgctg
      361 ctggccgccc gaaagctgcc cgcctggtag cttgtagaag cgcctcaggt cggggaagag
      421 gtcgaagatg aggtcgctat gccgtgccag atcgccccgc ttgcgctccg ctatcttcag
      481 gttctcctcc accgtgaggt cgcggaacac cgcccggtgc tccggcacgt agcccacgcc
      541 tcgagccgcg atggcgtagg gggtgaggcc ctgtatctcc tcgccgtcga acacgatttt
      601 gcccatgcgg ggcgatagta ggccgatgac cgacttgagc gtggtggtct tacccgcgcc
      661 gttgcggccc agcagggcgg tgatgctgcc cctcggcacg tccagcgaca ccccttccag
      721 tatgtggtac tggcctatga aggtgtggat ggcatccact gtgagcatgc tagacattgt
      781 cggtcccctc gctggtggct gccgttaaag gggccactcc gggcaactga ggctcggtgt
      841 agctctcgcc caggtaggcc tcctgcacgg cgcggctggt cctgatttcg tccggggtgc
      901 cctcggccag cacgctaccc tggtgcatga ccgttatgcg gtccgacacg ttcatcacga
      961 tacccatgtt gtgctcgacc agcaggatcg tcttgcccgt gtcctcccgt atgcgccgta
     1021 ttatgtcgag cagcataggc acctgctcgg aggccatgcc cgccgtcggc tcgtccaaca
     1081 gcagcacctg cgggccggct gccagcagga tagccagttc cagcttgcgc ttgtcgccgt
     1141 gcggcaatat ggaggctgcc tgccccgcgc gctcggacag ccccacctgc ttgagcgcgg
     1201 tcatggccgc ttcctcgtac tgccggaagc gtgtagccat ggaccagagg ttgaggctgt
     1261 cgcggcccat cgcctgcgcg gccagccggg cgttctccag cacggtgagg ttggggaaga
     1321 tattggtgat ctggaaggac cgtcctatcc ccatgtgggc catgcggtgc agaggcgtgc
     1381 ccgtgatgtc gcgcccgcgc aggaacaccc gccccctcgt cggcttgagg ttgccgctaa
     1441 tcaggttgaa cagggtggtc ttgcccgcgc cgttcggccc gatgatggag tgcagcgacc
     1501 cctcgcgcac ctgcaagttc accccgtcca cggcccgcaa gccgccgaag tgcttcgata
     1561 agtcacgagc ttcgatgatg taagtgtcca taagctttac ggagtgttga gttatgagtt
     1621 atgagttttg agttattctt gacaactcag cactcataac tcataactca taacttccta
     1681 aactacttgc agcgattcgc ggcggcgggt accgtgcacg ggggcgcggt ctcctccggc
     1741 ttgaacgctt tgaccagctc gaagaacctg tagtccttgt cggtggtgtt cttgagcttg
     1801 acgagggtca tcggttggag caggtcgtgg tcttcggcgc gaatggtgta agcacccttc
     1861 ggcccatcga acgtcatgcc ttccagcgcc tttatcaagc cctccgcatc ggcctcgccg
     1921 ttgttcatcg tgatggagcg caccagcatc tgcgccgcga tgaatccgct ctcggtgaac
     1981 aggtccggcg gtgtattgta cttggccttg tgcttgtcaa ccagccactt gttgatggcg
     2041 gtgtcgaaca ggctgtagtg gtacacgctc acgcccaccg agccaatcgc atcagcgtag
     2101 cccttggcca gcgtctggtt gtcgcccata cccgtggcta ccaccatgct gtcgaagata
     2161 ccaagctgct gcatctgctg gaacaggggc acgaagcccg tacccgccca cgtgacgacc
     2221 accacctccg cgcccgaatc gagcacctgg ttcaggtacg gggtgaagtc ggtggtgtcc
     2281 tgaggggcga acacggtgcc gaagtcggtg gtgttgtcgt tcaccacgaa ggtgccgccc
     2341 ttggccttga tgacgttgta gaaggccgcc gccgagccgc gcccgaaagc gtagtcgggg
     2401 gctatctgga tgaacttctt gcccatgccg gtcagcgcgg tgcccatcgt cagcgcgtcg
     2461 tgcacgctgc tgcggcctgc gcggaaggtg tagcgattga agttcttgcc ggtgatgtcg
     2521 ggggaggccg ccgggcctgc caggtagacc accttgttgt cgttagccac gctcgacacg
     2581 gccaacgcta cgcccgagct gggcacgccc accaggactt gcgccttgtc cttctctata
     2641 gcctcgcggg ccagggaggt gccggtatcg acagcgctcg cggtgtcctt ggtcgtgacc
     2701 ttgatgtcgc ggccgtagac cttattggtg ccgccggtgg catattcgag gcccagcgcg
     2761 aagccctgct cgagcatggg gccgtagata gccagcgaac cggagcggtc ggtgagcaag
     2821 cccacgttga ccgggccttt ggcggctgcc tgcttctgct tcaggcggaa ggtgccaagc
     2881 tgggagagga gcacgtcgtt gggggcctgg ttctcagggt gtagctcgaa caccgctcgc
     2941 tcgaagtact gcaccgtgta aggcttgccg ttgagggccg atacctcggt gaactcctgg
     3001 gagatggggt agccctgctg cgccagcccg ccgttcttgt tccagtagtc gaggaacttg
     3061 ccgcccacag tcatgccggt ctccgcgaac ctgcgggcac cggcggcggt gctcaccttc
     3121 tggccggccg gtcccgcagc accgtagatg gccttgtact ggaacacgcc caggagggag
     3181 aggagcacgt cgtaaggctt ctggttctcc gggtgcaact cgaacacggc acgctcgaag
     3241 tactgcgtct ggtaagtctt gccgtccgtg tcgctcctct ccgacatctc gtccgtgatg
     3301 gggaagccct gctgcgccag cccgccgttc tgctgccagt actccaggaa cctgcccttc
     3361 accgtcttac ccgtctccgg gaacgtctgc gagccggcct gcgcgtaggt gttggtgccc
     3421 ccgatgggga gcgccatgag cacaagcatc agggacgcca gtatggaaga ccatcgtttc
     3481 gcgttcttgg acattcgctt ttcctccttg ttcgtatggt aataaatcgc tccgcctgtc
     3541 ctcgccgcac ggtgccggga caaggcattc cccacccagg ttctcttcac gataggctga
     3601 cctcctattt gggtaatctc gaatttcgaa tttcgaattt tgtttatgcg tcttggcggt
     3661 tccgtctcct gttgggaggc gtttaccgcc cggctgccag gcgtaggtgc ttgagcagcg
     3721 cggcgttgta ctcttctggc ttctcgatgt ggggcgagtg gccggaatcg gtgatggtaa
     3781 gctcccagta gtcgccgccg ttggctttgt aggcgtcgag cacggcgcgg gtctgggcga
     3841 tcatgggctg gggcgggcat acttcggccc caggccagcc gggtaccgca ccgatctggc
     3901 ccaggaagcc gaagtcgaac agggaggtgt cggagacgat ctggtcgtcc gcgccccgca
     3961 cccacagcac gggggtcctg tgctcgatgt tggcgaacgc acccaggttg aggtacctgg
     4021 gcgacatggc gttgttgaag ccacccgtgc ccggcgcgac gttgggccag ttgggcgagt
     4081 cggtcatgcc gccggggtag aattcgtcgc caatacgggt cttgagcacc gaggtgacaa
     4141 aggcttcctc ccgctccggg gctactctga aaggcggctt gaagtagaag ttgttgagca
     4201 cgctgcgggc gctgaagggg ctttcgccgc cccggtcgcc ttccttgacc agccgcacgt
     4261 actcagggtt ggccgtgccg ccgcccgacc cggcgaaatc gtcgtggttg ggggtgccct
     4321 gcgcgtcctt cgtgccgcca aagccgtagg gggatacggg gctttccagg gtgagcgaga
     4381 gcaggtcggc cccgttgtct atcgcgtact gcatagccac gccgccgccc atcgaccagc
     4441 cgaggaggtg gaaccgcccc aggtcgagcg ccgttacgag cgccttgagg tcgtcggacc
     4501 agtcgcgcat accgcgagtg ccgtccaccg gcaccgcttc ggtatcgcca tagccgcgca
     4561 ggtcgggggc gagcacccgg taaccctcgg ccgccagggt cagcatagtt tcctcccaga
     4621 acggccccga cgacatattg ccgtgcacca gcaccacggg gtcgccatcc tgcggcccgc
     4681 tcaccagcac gtgcatcttg agccgcccgg tatcgacggt ggcctccgtg atgccgggta
     4741 acagcgttcc attagccata ctcaactcct tttgtgtggt tgtagattca gcagcgcggc
     4801 cgccggatag atgctccagc aacagccgca caaactcgtc cggcgcttcg atattgggcg
     4861 agtggccggt gccttgcagg atcacgtgcc cggcacccaa tgcctctgcc gcccgcgtgg
     4921 cgttggcccc gcccgtgagg gggtcgcggt cgcccgcgat gacgagggcc ggcacgcccg
     4981 cgtctcgcag cttatcgccc ggcttccagg cgacgagcgc ggggaggttg cgcagggcag
     5041 cctctatccg ctgctcgtgc ccctccacca ccagccgctg ccagtactcg tcgtccgcca
     5101 cgcctggcgc gagggctttg agggcggtcg cgaacatagc gcggtcagcc ttgatagctt
     5161 gctggcccgc cgggacgttg taggcttggg gcatgccgtc cacccacgcg ggcgacacag
     5221 ccacgaacgt cagcacgcgc tccggccact ccaacaagaa ctgcatggcg accgcgctgc
     5281 cgagcgagtg ccccaccagg tgcaccctgt ccaggcccaa cgcatcgagg aaggcgcgga
     5341 ggtcggcggc catttcgggc atggtgtagt cgttgtcggg gccttccgtc ttgccccgcc
     5401 cgcgcaggtc gtacgcgata cctcgatagc cgtccggcag gcgctccagc acaggctccc
     5461 accaggagga ggtgccccaa ttgccatgca cgagcacgat aggctcaccc tcaccttgct
     5521 ctatgtagtg cactctcaag ttgtcagcgt ccacaaacgg cattgcttag tccctgttct
     5581 tatctaatga ggcatacctt gcgtgctcgt ctcgcaacct gggaagaagg tgtttgctac
     5641 gatcttggcg gtctggcccc tgctggcgtc gtcgccgggg cggaaatagg gcctgttgcc
     5701 ctcgccgcac ggctcgctcg ccccgccgca ctcgtaaccg cttacatggc ccctgctcgc
     5761 caggcgttgc actccttcgt agaaggcgct gccgggtgct acgtcctcga agcgttgcgc
     5821 gccgggcgac tcggcgtaac ctgccgcgtc cgacacaatc ttcgctatct gcccgcgtga
     5881 ggcgctggcg ttcgggcgga agtacggcct gtcaccctcg ccgcaagctt cccctggccc
     5941 accgcacggg tagccgccga tgatgtgacg cgccgccatc ctctcgatga aggggtggta
     6001 ggtgctgccg gggggcacgt cctcgaaggt ctggccgccg acctgctcat tgaagcctgc
     6061 cgcgttggct acaatcttcg aaagctggcc ccgcgtgacg ttgctgcccg gcttgaaagt
     6121 gccgtcagcg aagccgccga gtatgccctg gcaggcaagg cagcgcacgt aagcgtagaa
     6181 ggcgctgccc tggggcacgt cggggaactg cacggcgcat gggatggcag tggcagtggg
     6241 cataggctca tttcctggca tcggattcgc ctggaagaac tgccacataa tcaggctggc
     6301 gtcgggaccg agcgggtcaa cgttgcggtc gccgccgttc gccgggtcga gtgtgccacc
     6361 cgaccacgcg tgtggcatag catcaacaat gtacttctcc agcacggtcc tgccgtcgcg
     6421 agagtcattg taggtatagc gggtataagt gtggccgccg ggcacctgcc ccggggaggt
     6481 accctccggc acgtcgtcaa tgtcgttgtt gtcggcaccg tcggcggcga ggtcgtcggt
     6541 ttgcgcccac tgggtaagga tgttgtgggc gttcttgacg acaaccaccg catcggcgct
     6601 accgtggaac acgatcacgg gcaccacgcg cttgtactgg cccatcgcgg cgtaggcttt
     6661 gttgccactc tctacggggt cggcgcagcc ccccgacgcc atcgccacta gaccggacgc
     6721 cgtatcggtc gcacacccgt actcgatgcc cgcgctcacc ccgatggctg cgtacacatc
     6781 cgggtaggtc gcgcccatga ttatgctcat cgccccgccc gccgagaggc ccgtagcaaa
     6841 gatgcgggtg ctgtcaaccg aatacctgct ctgcacctgg gctgtcatgc ccgcgatgag
     6901 tgcgggttct ccggcccccc gctgctggtg cgcaggctcg aaccagttcc agcaccttgt
     6961 gccattggcc gattgcggct ggcgcgggta gagggccagg aactggaact ggtccgcata
     7021 cacgttgaaa tgagtgtcca cggacatgga ggtggggtct tgtccgcagc catgcaacac
     7081 caccagaagc ggcagtggtg tgccggcctg gtagccctcc ggaatataca gccggtaatc
     7141 gcggtcacca tatgtttcgg tgatgaacga acctgcctgc acaacgccga aggttggcga
     7201 atcggtaagg ggcgcggcaa ccgcggtgcc atgccggcct gccgagctaa gtgccccggc
     7261 gagcagcaac agcgccaaga tgcaggcagc acctggaatc gcaaccggaa ttgagggctt
     7321 gtgggctttc aggaagtggt tcatagctta ccctccttgg gcatcctcta cccggtggaa
     7381 gaagtcgagc accacctggc agaactgcgg tatatcctca agcgctgaag cgtggccccg
     7441 gccctgcatt acgtgcaact cgctcctcgg cacccgctcc gcgatgtagc gcgacaacgc
     7501 gggcggggtc agcacgtcct cctcgcccac catgacgagc gtcggggcct gtatgtgcga
     7561 cagcctgtcg ctgatgtcgt gcagcatggc ccccttgatg aggttcatgg cccactcgat
     7621 aggcatgttg acgcccttct cgcggaagta gagcagccct tcgtagttct cgtccaggaa
     7681 gcgcccgccc cacacccagg gcgtggccac gtcgaagcgc aaggggctgc cccccgcctc
     7741 catcgcccgc acccacgagg tgagcttcac cttcagtatc gtgtccacat acgggtaagc
     7801 ccccgaagcc accgccgaga tcacccgctc cgagcggttc gcagcatagg ccagcaccgc
     7861 cacgccgccg ttagacagcc ccacgaagtg cgcccgctcc acacccaccg catccaccag
     7921 cgccgcaagg tcggctatgt gcagggccgt ctcgtacacg tggtccggct tgtcagactg
     7981 cccctgcccc cggcagtcgt acaccaggca gcgatactgc tcctcgaaga acggcaggtg
     8041 cttgttccag ctagccgtgt cggtaagcag cccgttcacg aacaccacgg tcggcccctc
     8101 ggccctaccc cggaactcgt aatacaggtt aatgtcgttg acactcactc ttggcatagt
     8161 tttcaccgat tttagatttt agattttgga ttttggattt ttaggcgttc ttctaaatcc
     8221 aaaatccaaa atctaaaatc taaaatcgcc atatctttct ttcagggcca gtttgtctat
     8281 cttgcccgcg cctgtaacgg ggagcgtgtc cgcgaagact acgctcttgg gtatcttgta
     8341 ccgggccagg cgctcgcggc agtaggcgat aatgccgtcg ccatccggca tgcagccgtc
     8401 gttgagcacc acgactgcgc ggcctacctc gccccagtgc tcgtcggtta cgcctatgag
     8461 ggcgaccgat gcgacagagg ggtggccgga gataacggcc tctatctcgg ctgggtagat
     8521 gttttcgccg ccggagatga ttacgtcctt aaggcggccc actatgtagt agaaaccttc
     8581 ttcgtcgcgc cgggcgaggt ccccggtgtg cagccagccg tccacgatag ttttggcgct
     8641 ctcttcgggg cggttccagt agccgggcac gacctgcggg ccgcgtatgt gcagctcgcc
     8701 cacctcgtcg gggccgcatt cgatgccgtc gggccggacc acccgcacgt ctacgtggaa
     8761 caggggcacg cccaccgagc cgggcttgcg ccgggcgttc tccgaagtca tccagaaggt
     8821 attggggcct gactcggtca gcccgtagcc catcttgaag tccacgcctc gcgcccacca
     8881 cttctcgaaa acgggcgggg gacagggcgc gccgccgctg attacccact tgaggcggga
     8941 gaaatcggcg gtggcccagc gggggtgggc ctgctgcgcg atgaacatgg tgggcacgcc
     9001 gaagtagatc gtgacgccca tatgctccac aaggtcgaac acctggtcca cgtcgaaccc
     9061 tcggcagacg atggaggtac cgccgatatg caccagcggc gcggtgagca cgttcagccc
     9121 gcccgtgtgg aacaggggca tgttcaggaa agtgcggtcg tcgggactca ggccccagct
     9181 catcaccgta ttgaccgagt tccaggtgac gttgccatgc gtgaggatag cccccttggg
     9241 gagtccggtg gtgccgcccg tatacgccag cgcccacggg ctgtcccagg taagctccac
     9301 gggaggcggc ggagagtcgg ggcaggcctc gcgctccaca aagagcctat cgccgggcga
     9361 ggctacctgc cgagggtcca gggccgcgta gtgctcgatg cccggcactt ccgactgtat
     9421 ggcccgcgcc tgtgctacga aatccggtcc atagatgagc acctgggggg tggcatcttg
     9481 caacaaggct accaactcgc gtcctgtgag ccgccaattg agcgtctgca taatcgcgcc
     9541 cagcttgccg caagcaaacc acacatcgag gtactcgacg cagttcatcg acagcaccgc
     9601 gaccagcgtg ccctgcccca ccccaagggc gcgcagccag ttagccgtgc ggttggccgt
     9661 ccggttccac tcgccgtaag tgataggttg gaactcgcgc tgcgcgtcga tgagagccac
     9721 cttccccggt gtcagcatct cccgccgcgc tagccagtcg ccaatataca tcttcatcct
     9781 cgaaggggtg caggggtctt cccctgacgg ggcgtggggt gtccccactt acccatcttt
     9841 tattctcttt ttctgtctca tagaaggaga gaggaggcgg ggacacccca caccccgtca
     9901 ggggaagacc catacacccc tatgtgccca gcactaagcc gccgtccacg ctgaggacgg
     9961 caccgttgat gaagcttgct tcctctgagg cgaggaagag gtaggcgtag gctatatcct
    10021 ccggctcgcc caggcggccg acgggggtgt gcgagaccat gttggagagc acctgttcgg
    10081 gcatggcgcg caccatgtcg gtgccgatga agccgggggc cacggcgttg acggtgatgc
    10141 cgcgcttgcc aagctcccgc gcccacacct tcgtcatgcc aatcacgccc gccttggtcg
    10201 ccacgtagtt ggtctgcccg aagttgccgt agatgcccac caccgacgac gcgctgacga
    10261 tgcgcccgta gccccgcgtt atcatgtggg gggcgaccgc ctgcgtgcag ttgaacacac
    10321 ccttgaggtt caccgacacc accaaatcga agtcggcctc gctcatcttg cccgtcacct
    10381 cgccgtcctt caccttcacc agttgggcgt ctcgtgttat accggcgttg ttgacgagga
    10441 tgtccacctt gccgaactgc tcgaccacag cggccacggc ggcttccacc tcggacgtgg
    10501 aggtgacgtt gaccttgtag aaggccgcct tgcccccggc gtcccgtatc tcggtgacga
    10561 gggcctgccc ctgttcctcg gccatgtccc acacagccac ggcagcttcc acctcagacg
    10621 cggaagttac gttgaccttg tagaaggccg ccctaccgcc gctgtctcga atctcggtgg
    10681 caagtgcctg cccctggtcc tctgccatgt cccacacggc cacggcggca ccggacgcgg
    10741 cgaatgtgaa cgctgtctgc cgccctatgc cgttcgcgcc gcccgtcatg accgctaccc
    10801 taccgtctag tctgaagttg gtccccatct gacaacactc gctccccagg tgtaacctat
    10861 gccggcggcc acgatgacca tgagatcgcc gtccttgagc ctgccgcttt caagcccctg
    10921 cacgatggaa ataatgccgt cctgctggcc catgtgcccg aagtcggaga gatagaaagt
    10981 ctggttgtcc cgcaggccaa gctccctgag cacgtaatca tgggcggaac gcttcatgtg
    11041 gaggatgttg aggtaatcga tgtctccgcg cgtgcagccg ctctcctcaa gggccatgtc
    11101 tatcaccttg aggaagttct gcatcgacag cgtgtcgagc cgctgcttca tcccttccgg
    11161 gtcgggcacg tcgaggtaat agcgccccgc cgacagccct tccgccgtga gcggctccat
    11221 cgtgccgccc gccgggatga ccacgtccag ggaaaatgag ccatcggtga tgatggcgct
    11281 cccaaggagg tggtttccgg ggtggttttt gcggaggatg agcgcacccg ccccgcagct
    11341 gagattgatc atgaagcggg agcgggggtt ggcgtagtcc accaggtcgc cgttgcggta
    11401 gccgcccgcg atgagcacgg tgttgacgtt cggctcgtag agcatcatcg cctttgccat
    11461 gcgcagggcc gccacggtgg tgccgcaccg cagttgcacg tccaccgccc aggcgcggtt
    11521 agcccctatg tcataggcca gcttgatgcc ggtggtccac agggggtact ctttgtattc
    11581 ctcggtggtg gacatcacca cgtcgatctc ttcgggccga acgtctgcgc gggagagggc
    11641 gtcctgggcg gctttgacag ccatcgcgtt agggtggtcg tcgggaccgg ggatgggctt
    11701 ccggcgcagc ccgaacttgc taacgagcac ctcttccggg atgccggtgt gctccgctat
    11761 ctcggccgcc gtgatgtaag tctggggtat gtacgcgccc gcggccacga tgccggctga
    11821 cgatctgctc atcctgcgcg cctttgctcc ggtggggacg gggaggaata acacgtaacg
    11881 agtaacgcgt actacgtaag ggtgcaataa gcaacaaagc ggcttatatg gtacacagcc
    11941 tcttgagccg cgttacgtgt tacgtgttac tcgttacgct cggttgagca agagcctacc
    12001 agaggggggt tacaaaacgg ttacagggag gtcttcggcc ttgaggaggc ggttgtagag
    12061 ggcggtggtg gcggtggagg gtgcgacgcc aagctcggcg gacatggtag tgcggcaccg
    12121 ctggtaggcg cgcagggcca gcgtccggtc gcccctgtgt gcgtaggcca gcatcatcca
    12181 gcggtaagcc ccctcccagc aagcgtcata agagaggatg cgggcgcaaa cctccagcgc
    12241 ctcgtcgtat tgcccgccct cgaccagcag gcccgcgagc cggtcggctg ccctcagaaa
    12301 aatggagagc agccgttcgc gctcggcact ggcccagtcc tcgtagaggg catcaggtag
    12361 gtagttgccc gcgtacaggt caagtgccag gcgcaggtta gcaacagcct cctcgctctc
    12421 cctggcgtcg gccaggcgcg acccggcctg gcagcggcgc tcgaactcgt ccgtatcgag
    12481 ccagaggtcc gcctccgggc ggaggcggta ggagacgcca tcgcgcacga tgtaggcgaa
    12541 cgccgcgtcg gaggcgtgcc ccggctcaat cgccttgttg agggcgttga gggccacttt
    12601 gaagtcgcgc agggcggctt ccggcgagag gttgggccac aagcgctcca ccacctcatc
    12661 acgctgcaac caccggcccc gctgggtgag cagaagctgg aatagctgcc tcgccttgtc
    12721 tcgctgccac tctctcggct ccacctccac gtcaccccgg aacacgcgga aggtgccgag
    12781 taggtacacg cgcaactggt agcctgggtg gacctgcgcg tcgggcaggc cgatctgaga
    12841 cagcaggcga gagatatagg ctgcgttgcg gcgattgcca cgggcgcgca ccagcagtgg
    12901 caccgcgcgg cggatgtcgg gaggcccaag gagggaggga gccgtgaaaa ggaagtcgta
    12961 gccgtgggcc tcgcagaggg ccagcgcctc gtcaatggag acgttgcaga gttccgcctg
    13021 acccaggtcg tgataggcga gcgccagcca gaggcgtgtg gccgcgcggc caaagctatc
    13081 gccacaaccc cagaaggaca cgagcacctg ggacaggagg tctgacgcgc gttgtggttg
    13141 cccccccagc acgtaactcg cgcccagcgt tagctcaatc agggccacta tccaggcgtc
    13201 gcccgcccag cggccaatct cagccccctc ggcagcggag cgctcggcgg aagccaggtc
    13261 gccgctgaag ccgtacgtcc gcgtgaggcc ccacatcgcc tcgccgcgca tgcgccgcac
    13321 ggccagctta tcgcccaggg ccacggcggc cccgtagccg cgtatcgcct cctcgacgtg
    13381 ttgcgcgttc ggggccgggg aggttagatc gcggcgaatt tgcagggcgt ggccgagccg
    13441 ggtctgcccc accgcctcga tgaagggcga gtcgagccgc cgggccacgt cgatggcctc
    13501 ctgggcaagc tcgatcgcgc gctccggctg ccccctgaac gagtggatga gcgacagtat
    13561 cagcagggtc tcgcggtggg agcgcggcgg gtgaacctgg cccaactcga cctcccggcg
    13621 ctcggcctcg gcccactgct cgagcgtgcg ttgcgcctcg tccagccgcc cggtgcgcaa
    13681 cttcacccgc acgctgagcg tgtcctcgcc cggcccctcc tcgcgcagcg aacgagcctc
    13741 cgaacgtagt tcctccgcct cggacggttt gcccatattg agcttgttct cggccagcaa
    13801 ttcgagcagg cgggcgcgcg attcgcggtc cggcaggccc tcgatgagac gcagcgcctc
    13861 ctccaacagg ctctcggcct gagcggggcg taccgtatcg aggtacaaca gggcctgtcc
    13921 gcgcaaggcc cggctcgtgc ccgcccggtc gtccctggcg cgccacaccc gttcggcctg
    13981 cgcgtaccag gcgagcgcct catcgaagcg gctgcgcatc cggtagatgt cgcccaggaa
    14041 agcctgtaag agcgggtggg cggccagcac gtcgggcggc aggggatcga tccaggtggc
    14101 gaccgtgtcg agccagcccg cgcgcagggc agtctcgccc gcccgctcta tcgcgcccgc
    14161 cacctgggag aggtcgcccg cggccagcca gtgatagatc gcctcctcgt agtcgcccgc
    14221 gcccgcgaag aactccgccg cgaggtggtg gcgcttgagc gtgtcctcag catcggcctg
    14281 cgactgctgc cggaggaaat cgtggaagag gtgatggtag cggtagtgcc gctctgaggg
    14341 gtctcccagg gccaccataa acaggtccat ctccagcagg cggtcgagca tagcaccgct
    14401 gtctgaacag cccgtgacgg catcgcaggc ggcgggggta agctcgcgca ggatggcggt
    14461 atcgcgcagg aatgaagcga catcagtagg ttgtgcccca agcacgtcgc tcgccaggta
    14521 atcgaacagg gcactcaggg aggtggagcc ttgttcgagt agctgagagg tgctgcgggt
    14581 ggtgccgcct ctcaggccct gccacaccag ttgcagggca atcggccagc cctccgtctt
    14641 ctccacgagc gcgtgtagct cctcaggggc aagctccagc ctgtagacat cgcggaagag
    14701 cgccgccacc tcctcgtgcc cgaaggccag cgccttgcgg ccgatctcca gcacctcgcc
    14761 ccgcgcccgc caggtcacca gcttggggaa cgcaaggggg tagcgggtgg agacgatcac
    14821 gtgcaggttg gagggcaggt actccaggaa tctctcgacc agagcggtaa tctccggcga
    14881 gttgccgacg aagtggtagt cgtcgagcac cagcagggca ggctcgcccg gcccttcggc
    14941 aagggcgttg atgagggcgt cgagggcctg cacccaggcg gccttgctgc cctcgctgcc
    15001 gcgctcgcgc aagaccacga ggggcaggtc cgacataccc ggcatgcgca cgcggaaagc
    15061 cgagatgagg taggagagga agcgctgggg gtcgctctcg gcatcgccca gggtgtacca
    15121 gaagaggggt atctcgccgt tgtcgagagc ggcaagggcg gtggtcttgc tgtagccggt
    15181 gcccgcgtgg agcatcgtga ggcggtactg cagcgcctcg cgcaggaggg aggcgagggc
    15241 agggcgaggg agcacgcggc tgtgcacctg gggtggggcc agcttagtgc gcaggaggag
    15301 gtcttcgcgg cgtgtggggg ccgggtacat aggctcactc acatagagtg cgtggagggg
    15361 gtgcgtctca agccctccgt cgatacgcag aagggcatgt tgtgggacat tatgctccaa
    15421 tagcgacggt tgtgcaaggc ccggcttggg gaggaggcga cagcgtactg cgtgccctgt
    15481 gaggtggcgg agggacggta taagcgtaga gatcaagctt gtggcgaaga ggcacactgc
    15541 ttagacgccg ggcggggaac ttgcgcaggt aggcccttct gccttatttc cggcctctac
    15601 tccagcattt tgctccctgg tttggaggtg aacaaggcct caggtaagtt gtacttgtgt
    15661 gtacttgata ttaaagtcta ataacacttg acaaataata acactgctcc tataatctga
    15721 ccagatgcag cgcttcggct tatgaggtgc tgcaagcgga ataacactgg gccaccacgg
    15781 gtagaggcgc gtgattatgc aaacgccttt atgtcgggag gtactttctc gtgtctggtt
    15841 tcagacgaaa ggtaacaaga tggaaatctc gaagggtata aagaaggctt tggttggcgc
    15901 gctcttattc ctggttcttg gtgtgtctgc cctcagtggc ttcagcgtcc agcagcacgg
    15961 cgcggtgagt gatgtgcctg cggcgtacgc ggggccaaac cctcctcctg actacggtaa
    16021 gcccccgacg gatccgaatg cgccaacgcc tactcccacc gcaaccccct tcccgtagtt
    16081 ccggtgcaca cggcatcaga agtgaagaaa tagagtctga cctctaaccg tcgaacgatg
    16141 agccatccca taaaccggat ggctcatcgt ttatattggt tcctaatttt ttttgcgtta
    16201 ttcatgtccg ttgggatttg ccttaagcag tctttgcgaa tatgacccct taccggtata
    16261 tctataatga tagagcagct attcacagga ggaagcaaga tatggatatg ggactgaagg
    16321 gcacagtagc agttgtcatc gcatcaagtc aggggctagg taaggccacg gcactgggat
    16381 tagcagaaga aggggccaat ctagtaa
//
LOCUS       DASRWZ010000156         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_423927, whole genome shotgun sequence.
ACCESSION   DASRWZ010000156 DASRWZ010000000
VERSION     DASRWZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423927"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="VFH60_02320"
     CDS             <1..1623
                     /locus_tag="VFH60_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312644.1"
                     /translation="ASELASAGVRWIRMEFFADYDGSVRPGEINWEKYDWFIRELAPK
                     YNLKVLGLLNVGMVAFNGETLRTLAFNDPADGGGPDPTNGSNHFIRVFTARAQTIAAR
                     YGTAISAYEIINEPNISFDLWLDSGHGSAEIKPERYAALIFNAYRAIKSVNAQAQVIV
                     GGIMIGSPPEGQDHDQFDYLFQLYVSPWAEKYRADGYSSREGWNVVPWDGVAVHPYFL
                     DPPQLFELLREFARKLRDRGDHASKLWITEIGMQAVPPANRGDAPTDDEVRQAEFVRG
                     LYGGILRDSELRSIVPHVFWFKYEDFVPGSYTHNYGLVRLQENERRDGYSPTGAVDVR
                     KLAYRAYQEIARGGAITDPVDEGQVLDGNQVYFGETGQAIAPQFAQYWRDHGGLGLFG
                     YPISRPVPLNGYLSQFFERAIFEYHPENRGTPYEVQLRLLGNEFTQGRSFDRADVSLL
                     TPDKVYFPETGHSLGGAFLKHWQENGAMHVYGYPISEEITEVSPTDGLPYLVQYFERN
                     RFEYHPEAAGTPFEVQLGLLGANMLKLDLWWR"
     gene            complement(1821..2285)
                     /locus_tag="VFH60_02325"
     CDS             complement(1821..2285)
                     /locus_tag="VFH60_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET6312645.1"
                     /translation="MPDITGVVVKELVTHTDERGFFRELIRKSDDFFVEGFAQLSHSL
                     MHPGVAKAWHIHKHQIDWWYVPVGVLKVALYDTRPDSPTKGVLQELFMGGDYGQKILK
                     IPPGVAHGCKAIGGPAHLVYVTSNTYDPADEGRIPHDDPEIGYDWVSLPSIK"
     gene            complement(2359..2748)
                     /locus_tag="VFH60_02330"
     CDS             complement(2359..2748)
                     /locus_tag="VFH60_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312646.1"
                     /translation="MINQNEPEGKMDAAPEPESAPVALLFCADLMFAVQLQNMARKSG
                     YRVANARPGAPLPGADVLVVDLGSRGDWEAAVKEMAGRSVPVIAFGPHMDAEGRKRAK
                     AAGATRVLANSNLTRDLPTILADLRER"
     gene            2848..4623
                     /locus_tag="VFH60_02335"
     CDS             2848..4623
                     /locus_tag="VFH60_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6312647.1"
                     /translation="MFARESSEGYRGSMQLGQFWDGPDNFGWQNLASRLEEQTRVFWA
                     IDSISTAITSTQTVQEMLQSALERIAEVTGAHRGGIYVPQPDGTWLLAVRHNMPEDPT
                     EKRAVLAPDHLALRLVLESNGAITRSERLDEPSSLISQAAKEAGTQSWAAITIMALNT
                     IWGVLTLSSHNYNAFNSVHLELLRVVGQLLGLSISNTVMHEKALVAADSEFRRRVAEM
                     ETVLGSMSDGLLMCDNSGTIVRANRAAARILGTSMSSLVGSCVLTDAWRHSTYAIDIE
                     AETEIDWPLLRVFEDGDECRNYHIERVVRDEVRVLSLTASPVRRPGGGRDGAVILVRD
                     ITEEREAEEMKEDFLSILSHELRGPLTVISGYAQMLARRLGRHGLSEELNYVSLIKEN
                     ALRMSGMVGDLVDSGRLESGVQAIKKEPADLGALVMAVAARISTEQSHAANFHSIDVR
                     IEPDLPHVEMDARRIDQVLTNLITNGVKYSPDGGPITIAVERTPPEKYIVVPIGDNTT
                     GELAPESLMVSVTDEGAGVPPEERKRIFDRAYRGDRGKEISAQGLGLGLYISRLAVEA
                     HGGHIGVEEGPGGIGSTFWFTVPLE"
     gene            complement(4604..>5199)
                     /locus_tag="VFH60_02340"
     CDS             complement(4604..>5199)
                     /locus_tag="VFH60_02340"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018975170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6312648.1"
                     /translation="DIEKRHEGRADFPLTELGREQARLAAAWIAISCLPTAIVSSPLK
                     RASETARIVGAACGLMVEFDEALMEFNNGALAGLSFEEADRLYPLPDGGRPPHERVPG
                     GESLLEFRFRAEEVWSRLVAQHAEAGRLLIVAHGGMISMLFRSFARLPVGIDVWLHTG
                     DTGMHLWRLDGAVRSILFANRLGHLDGPGGPVTPAAP"
BASE COUNT          996 a         1639 c         1642 g          922 t
ORIGIN      
        1 gcttcagagc ttgcatcggc aggcgtgcgc tggatacgca tggagttctt tgccgattac
       61 gacggctcgg tgcgccccgg cgagatcaac tgggagaagt acgactggtt cattcgagag
      121 cttgcgccca agtacaacct gaaggtgctg gggctgctca acgtgggcat ggtggccttc
      181 aacggcgaaa cgctgcgcac cctggccttc aacgaccctg ccgacggggg tggtcctgac
      241 ccgaccaacg gctccaacca cttcatccgc gtgttcaccg cacgcgccca gacgattgcc
      301 gcgcgttacg gcaccgccat cagcgcctac gagattatca acgagccgaa catcagcttc
      361 gacctgtggc tggacagcgg gcacggctcg gctgagatca agccggagcg ctacgccgcc
      421 ctcattttca atgcctaccg ggctatcaag agcgtcaacg cccaggccca ggtgatagtc
      481 ggcggcatca tgattggctc gccgccggag ggccaggacc acgatcaatt cgactacctg
      541 ttccagcttt acgtctcgcc ctgggccgag aagtaccggg ccgacggcta cagcagccgc
      601 gagggctgga acgtcgtgcc gtgggacggc gtggccgtgc acccctactt cctcgacccg
      661 ccgcagcttt tcgagctgct gcgagagttc gcccgcaagc tgcgagaccg tggggaccac
      721 gcgagcaagc tctggatcac cgagatcggt atgcaagctg tgccgcccgc caaccggggt
      781 gatgcgccca ctgacgatga ggtcaggcag gccgagttcg tgcgcggtct gtacggcggc
      841 attctgcgcg attcggaatt gcggtccata gtgccgcacg ttttctggtt caagtacgag
      901 gacttcgtgc caggcagcta cacgcacaac tatggcctgg tgcgcttgca ggaaaacgag
      961 cgccgcgacg ggtatagccc aacaggggcc gtggatgtac gcaagctagc ctatcgcgcc
     1021 taccaggaaa tcgcccgtgg gggcgctatt accgacccgg tagacgaggg tcaggtgctc
     1081 gacggcaacc aggtctactt tggcgagact ggccaggcaa tcgccccaca attcgcccag
     1141 tactggcgcg accacggcgg gctggggctg ttcggctacc ccattagccg ccccgtgccg
     1201 ctgaacggct acttgagcca gttcttcgag agggccatct tcgagtacca ccccgaaaac
     1261 aggggcactc catacgaagt gcagttgcgg ctgctcggca acgagtttac gcaggggcgc
     1321 agcttcgaca gagccgacgt ctccttgctg acgcccgaca aggtgtattt cccggaaacg
     1381 ggccacagcc tgggcggtgc cttcctgaag cactggcaag agaacggggc catgcacgtc
     1441 tatggctatc ccatctccga ggagataacg gaagtgtcgc ccacggacgg cctgccgtac
     1501 ctggtgcagt acttcgagcg caaccgcttc gagtaccacc ccgaagccgc gggcacgcca
     1561 ttcgaggtgc agctcggcct cctcggggcc aacatgctca agctcgacct ctggtggcga
     1621 tgagataaga ttgacggaac cgccaagacg gaaaggacgc caagagcgcg ccaagatagt
     1681 tgaggtcttg gggtactaag aggaccaggg tacgatagtt gtaccctggt cctcctgctt
     1741 agaacaccct tatcttaatc ttggcgcgct cttggcgtcc ttggcatctt ggcggttccg
     1801 tctcgtgtta ttccactagg tcacttgatg gaggggaggg acacccagtc gtagccgatt
     1861 tcggggtcgt cgtgggggat gcggccttcg tctgcggggt cgtaggtgtt cgaggtgaca
     1921 tagacgaggt gagcgggacc gcctatggcc ttgcagccgt gggccacgcc gggagggatc
     1981 ttgagtatct tttgcccgta gtcgccgccc atgaacagtt cctgcaagac ccctttagtg
     2041 ggcgaatccg ggcgggtatc gtagagggcc accttcagca cgcccaccgg cacgtaccac
     2101 cagtcgattt gatgcttgtg gatgtgccag gccttggcga cgccggggtg catcagcgag
     2161 tggctcagct gggcgaagcc ctcgacgaag aaatcgtccg atttgcgaat aagctcccgg
     2221 aaaaagcccc gctcgtccgt gtgtgtaacc agttctttca ccactacgcc cgtaatatca
     2281 ggcattgtca caacccctct ccgtcaaaag tcgagcgggc aaagagacca cgctcgtaat
     2341 tcgcaatgtg taattcggtc accgctcccg caggtcggca aggatcgtcg gcaggtcgcg
     2401 tgtcaggttg gagttggcga gcacgcgggt agccccggcg gctttcgccc gcttgcggcc
     2461 ttccgcgtcc atgtgggggc cgaacgcgat cacgggcaca ctcctcccgg ccatctcctt
     2521 gacggcggct tcccagtcgc ctcgcgagcc gaggtccact accagcacgt ccgcgccggg
     2581 caaaggagcg ccgggccggg cgttagcgac cctgtagccg ctcttgcggg ccatgttctg
     2641 caactgcact gcgaacatca ggtccgcgca gaacagtagc gctacaggcg ccgactccgg
     2701 ttctggtgcc gcgtccatct ttccttccgg ctcgttttgg tttatcatat ttcttgcacc
     2761 atgtgaattt gagagtagag caccctggca ggcgcagcac cggactgcat tatgcaagcg
     2821 gggcgctact cgtggcattt gtatcagatg ttcgcacgcg agtcaagcga aggttaccgg
     2881 gggtcgatgc agttggggca attctgggac gggcctgata attttggctg gcagaacctg
     2941 gcctcacgcc tggaggaaca gacgcgcgtc ttttgggcca tcgactctat ttccaccgcc
     3001 atcacctcta ctcagacggt gcaggagatg ctgcaaagcg cgctggagcg catcgcggaa
     3061 gtaacgggcg cgcacagggg cggcatctac gtgcctcagc cggacggcac ctggctgctc
     3121 gcggtgaggc acaatatgcc cgaagaccct accgagaagc gcgccgtcct ggcacccgac
     3181 cacctcgcgt tgcggctcgt gctcgaatcg aacggcgcta tcacgcgcag cgagcgatta
     3241 gacgagccta gcagtctgat ttcccaggcc gccaaggaag caggcactca gagttgggcc
     3301 gcgataacca tcatggccct caacaccatc tggggcgtgc ttaccctttc aagccacaac
     3361 tataacgcct tcaacagcgt ccaccttgaa ttgctacgag ttgtggggca gttactcggc
     3421 ctgtccatct caaacacggt catgcacgag aaggcgttgg tcgccgcgga cagcgagttc
     3481 cggcggcgcg tggccgagat ggagaccgtg ctcggcagca tgtccgacgg gctgctcatg
     3541 tgcgacaact ccggcacaat cgtcagggcc aaccgcgcgg cggcccgcat cctgggcacg
     3601 tccatgtcga gcctggtcgg tagttgcgtg ctgaccgacg catggcgtca cagcacctat
     3661 gccatcgata ttgaagcgga gacggagatt gattggcccc tgctgcgggt attcgaggac
     3721 ggcgacgagt gccgcaacta ccacatcgaa cgggtagtgc gggacgaggt gcgggtgctc
     3781 agcctgacag cctccccggt gcggcggcca ggcggcggca gggacggcgc ggtcatcctg
     3841 gtgcgcgaca tcacggagga gcgcgaggcc gaggagatga aagaggactt cctgagcatc
     3901 ctctctcacg agctgcgcgg ccccctgacc gtcatcagcg gctacgctca gatgctcgcc
     3961 cggcgactcg gcaggcacgg cctcagcgaa gagcttaact acgtgtcgct catcaaggag
     4021 aacgccctcc gcatgagcgg catggtgggt gacctggtag actcggggag gctcgagtcg
     4081 ggcgtgcagg ccatcaagaa ggagcctgcc gacctgggcg cgcttgtcat ggcggtcgcg
     4141 gcgcgcatca gcacggagca gagccacgcc gccaacttcc acagcatcga cgtgcgtatc
     4201 gagcctgacc tgccacacgt cgagatggac gcccggcgca tcgaccaggt gctcacgaac
     4261 ctcattacga acggcgtgaa gtactcgccc gacggtggac ctatcacaat cgcggtggag
     4321 cggacgcctc ccgaaaagta catagtggtc cccataggtg acaacaccac cggcgagctg
     4381 gcccctgaga gcctcatggt ctccgtcaca gacgaggggg cgggagtgcc gcctgaagag
     4441 cgcaagcgca tcttcgaccg cgcctacagg ggcgacaggg gcaaggagat cagcgcgcag
     4501 gggctgggac tcggcctcta catttcgcgc ctggcggtag aagcgcacgg gggccacatc
     4561 ggggtagagg aagggccggg agggataggc agcactttct ggttcacggt gccgctggag
     4621 taaccggccc accgggtcca tcaaggtgtc ccaggcgatt ggcgaagagg atgctccgca
     4681 ccgcgccgtc cagccgccac aggtgcatgc cggtgtcccc ggtgtgtagc cagacgtcga
     4741 tgcccaccgg cagccgcgcg aacgaacgga acagcatgct tatcatgcca ccgtgcgcca
     4801 cgattagcag tcgccccgcc tccgcatgtt gcgccaccag cctcgaccag acctcctcgg
     4861 ctcggaaccg gaactccaac aacgactccc caccaggcac ccgttcgtgg ggtggcctgc
     4921 ccccgtccgg gagcgggtac aggcgatcag cttcctcgaa cgacagcccg gcgagcgcgc
     4981 cgttgttgaa ttccatcagc gcttcgtcga attccaccat cagaccgcac gcagcaccca
     5041 caatccgggc tgtctccgag gcgcgtttga gtggactcga cacgatggcc gtgggaaggc
     5101 agctgattgc aatccacgcg gcagccaggc gcgcctgctc cctgccaagc tcggtcaagg
     5161 ggaaatcagc gcggccttcg tgcctctttt cgatgtcag
//
LOCUS       DASRWZ010000157         3375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_904015, whole genome shotgun sequence.
ACCESSION   DASRWZ010000157 DASRWZ010000000
VERSION     DASRWZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904015"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFH60_02345"
     CDS             complement(<1..304)
                     /locus_tag="VFH60_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312649.1"
                     /translation="MKLDPSRPANRTSSTRLRSRPDPHLALRLGFALVVPALILAWSL
                     VTVLGGESKRELSGAAYAPGVRASARSGNVSSQARQAAATPTIEVYDPNKAQPSTPT"
     gene            complement(524..1720)
                     /locus_tag="VFH60_02350"
     CDS             complement(524..1720)
                     /locus_tag="VFH60_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET6312650.1"
                     /translation="MPSQLPAHEQHPPPGTLGEVAALFLKLGTIAFGGPVAHIAMMRD
                     EVVTRRRWVTDQQFLDLLGATNLIPGPNSTEMAIHLGFVRAGWRGLVVAGSLFILPAM
                     LIVIALAWLYVQYGSTPGAGWLLYGIKPVIIAIVAQALWGLLKTAVKGPLHAIVGAGV
                     LALYLLGFNEILLLFGGGLLVMLLENGLRDWRARSQAASFVPLFGAGPLLAQIAAQGA
                     YGLASFNLWTLFLTFLKIGAVLYGSGYVLLAFLRNDFVERLGWLTNQQLLDAVAVGQL
                     TPGPFFTTATFIGYLLGGLPGALVATLGIFLPSCVFVAVSGPFVRRLRRSRWSASLLD
                     GVNVAALGLMLGVTWELGRSALVDLPTVLIAVVAWAALLRFKVNSAWLVLAGGGVGLV
                     GFMLSP"
     gene            complement(1781..1877)
                     /locus_tag="VFH60_02355"
     tRNA            complement(1781..1877)
                     /locus_tag="VFH60_02355"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1841..1843),aa:Sec,seq:tca)
     gene            complement(1981..2472)
                     /locus_tag="VFH60_02360"
     CDS             complement(1981..2472)
                     /locus_tag="VFH60_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312651.1"
                     /translation="MSIQDFYSAASQISFTLLGFWWAVAQFRYADWAGDPARRRLAHV
                     VSLLFLLPGVMSLVSMLSGDTPVLWRLTFGGAGVLGLVATLLIVTGTPAESAGGWLRP
                     AALFVAAPLYALITLFAIWPEFARDWLGLQPLQVEGILLSLLVFLGVNLAWSLFWEGK
                     HSG"
     gene            complement(2522..>3375)
                     /locus_tag="VFH60_02365"
     CDS             complement(2522..>3375)
                     /locus_tag="VFH60_02365"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET6312652.1"
                     /translation="MAGIGSRFVAAMLDHLILAVTLFLLVCAASTIGLISLSARDGGA
                     GAFLVLAILTLITFLIIWGYFVVFETVWNGQTPGKRAGRLRVIRYNGQPIGAGEALVR
                     NLVRLVDFMPGFYGVGLLSMFIDKNARRLGDFAAGTIVIREGEHTRLHDVRVNRPAPE
                     QPSYAPTVPASTPYSPYTPYTSYSPYATGQIRNPQSEIQNRPDPLPGISIRDVTHQDY
                     ALMRELLARSVRGELPSDRTQDLAVRLAHGVAGRMGQNFAEWQARGWQPLVFLQAVLD
                     AKDVRGE"
BASE COUNT          694 a         1052 c         1143 g          486 t
ORIGIN      
        1 gggtgggggt ggaaggttgg gccttgttgg ggtcgtacac ctctatcgtc ggagtagcgg
       61 cggcctgcct cgcctgggac gagacattcc cgctacgtgc ggaggcccgg acacccggag
      121 cataagccgc cccggatagc tcccgcttgc tctcgccgcc caggacggta accaggctcc
      181 aggcaaggat gagggcgggc acgaccagcg cgaagccgag cctgagagcc aggtgagggt
      241 cggggcggct ccggagacgc gtagaggagg tgcggttagc gggacgtgat gggtcgagct
      301 tcaaggtggt atacctgcca gggagttagc gttcgggcgt ctgcataata tacagcgaac
      361 agggtctgcg aagttcaatt agcgcgcgtg cggagcggga caggtcgtgc ggcggcaaag
      421 agtgtagggg tagtagcgcg ggtgctacct ttacaattta cagcatgcac aggggcttga
      481 aatcggacca gcgaggtatt ccgggtagag cgcgagtcgg gtgctacggg cttagcatga
      541 aacctacgag gccaacgccc ccgcccgcaa gcaccagcca ggccgaattc accttgaacc
      601 ggagcagggc agcccaggcg actacggcta tcagcacagt cggcaaatct acaagcgccg
      661 acctgccaag ctcccacgtg acccccagca tcaggcccag ggccgccacg ttcacgccgt
      721 ccagcagcga ggcggaccag cgggagcggc gcaaacggcg cacgaacggg ccgcttaccg
      781 ccacaaagac acacgacggc aagaatatgc cgagggtggc gaccaacgcc cccggcaggc
      841 cgccgagcaa gtagccgatg aaagtggccg tggtgaagaa ggggccgggg gttagctggc
      901 ccaccgcgac cgcgtcgagc aattgctggt tggtgagcca ccccagccgc tccacgaaat
      961 cgttgcgcag gaacgcaagc agcacgtagc cgctgccata gaggaccgcg ccgatcttga
     1021 ggaaggtcag aaacagggtc cagaggttga aggaagcaag cccgtaagcc ccctgcgcgg
     1081 ctatttgggc cagcagcggc cccgcgccga acagcggcac aaaggaagca gcttgagagc
     1141 gcgcccgcca atccctcaac ccgttctcaa ggagcataac gaggaggcca ccgccaaaca
     1201 gcaggagaat ctcgttgaag ccgaggaggt agagcgccag cacgcccgcc cctacgatag
     1261 cgtggagggg gcctttcacc gccgtcttga gcaggcccca cagcgcttgc gccactatcg
     1321 cgatgattac gggcttgatg ccgtagagca gccagcctgc ccccggcgtg gagccgtact
     1381 gcacgtacag ccacgccagc gcgatgacta tcagcatggc gggcaggatg aagagggagc
     1441 ctgccaccac cagccctcgc cagcccgcgc gcacgaagcc gaggtgtata gccatctcgg
     1501 tcgaattggg gccggggatc aggttggtgg cccccagcag gtcgaggaat tgctggtcgg
     1561 tgacccacct gcggcgagtg accacctcgt cgcgcatcat cgcgatatgt gccaccgggc
     1621 cgccgaacgc gatagtgccc agcttaagga aaagagcagc cacctcgccc aaagtgccgg
     1681 gcggggggtg ctgctcgtgc gcgggaagtt gagagggcat atcctcagtc ggctgtagcc
     1741 tgtcggtcat cgcctggcct gcataccggg gctgcccacg tggcgagggt gcatgggagt
     1801 cgaacccacc tgcgccactc agcgtaacgc acagcagttt tgaagactgt gaagcccacc
     1861 gggacctacg cacccccgcc ttaagcatac ccaacgtagc gagggagcgt caaggcaggg
     1921 catggagagt tgcgagctga gtgtcgctaa cggggcactg ccggtgctcc tgtggaggcc
     1981 ctagccgctg tgcttgcctt cccaaaacag cgaccacgcc aggttcacgc ccaaaaagac
     2041 aagcagcgac agcaggatgc cctccacttg caagggttgc agaccaagcc agtcgcgggc
     2101 gaattccggc catatggcga agagggttat gagggcgtag agcggggcgg ctacgaacag
     2161 ggcagcgggc cgaagccagc ccccagcact ttcagccggg gtgccggtga caatcagcag
     2221 ggtggcgacc aggcccagca cgcccgcccc gccgaaggta agccgccaca gcaccggggt
     2281 gtcgcccgaa agcatcgaca cgagggacat gaccccaggc agcaagaaca gcagcgacac
     2341 cacgtgcgcc agcctgcggc gtgccgggtc gcccgcccag tcggcgtacc ggaactgcgc
     2401 caccgcccac cagaagccaa gcagggtgaa gctaatctgc gacgcggcgc tgtagaagtc
     2461 ctggatggac atttctcccg tctccttcga gcagccgctt caaccgcagc tgcagggtga
     2521 gctactcccc acgcacgtcc ttggcgtcga gcacggcttg caaaaagacg agcggctgcc
     2581 agcctctagc ctgccactcc gcgaagttct gccccatgcg gcccgctacg ccatgtgcca
     2641 ggcgcacggc gaggtcctgc gtacggtcgg aggggagttc gccgcgcact gaccgggcga
     2701 gtagctcgcg catcagggcg tagtcctggt gggtaacgtc gcgaattgag ataccgggca
     2761 gagggtcggg acgattttgg atttcggatt gcggatttcg gatttggccg gtggcgtagg
     2821 gcgagtacga ggtgtagggc gtgtagggcg agtagggagt ggaagccggg accgtagggg
     2881 cgtaggaggg ttgttccggg gcgggccggt ttacgcgcac gtcgtggagg cgggtatgct
     2941 cgccctcgcg gatgacgatt gtgccagccg cgaagtcccc caggcggcgg gcgttcttgt
     3001 cgatgaacat gctcagcagg ccgaccccgt agaagccggg catgaagtct accaggcgca
     3061 ccaggttgcg caccagcgcc tcacccgccc cgattggttg gccgttgtag cggatgacgc
     3121 gcagcctgcc cgcgcgcttg ccgggcgtct gtccgttcca caccgtctca aacaccacga
     3181 agtagcccca gatgatgaga aacgtgatca gcgtgaggat ggcgagcacc aggaacgcgc
     3241 ccgcgccccc atccctggcc gataagctta tgaggccaat cgtggaagcg gcgcacacca
     3301 ggaggaacag cgtcacggcc aggatcaggt ggtccagcat cgcagccaca aaccgcgagc
     3361 ctatccccgc catct
//
LOCUS       DASRWZ010000158         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1246636, whole genome shotgun sequence.
ACCESSION   DASRWZ010000158 DASRWZ010000000
VERSION     DASRWZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246636"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFH60_02370"
     CDS             <1..614
                     /locus_tag="VFH60_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6312653.1"
                     /translation="AKYQLPVYRYDPAAANRLLDEAGWARGADGIRVKDGERLSFEYG
                     TTRNATRQAIQALVQADLKVVGVEAITVNYPQGFFAQDGPVVTGKTKLAQFAFVNDEL
                     TGFSHFSNDPVLSNVEPTTRNYQQYRNDRVTEANRIFNSEIDRGKTAEAAAIIQVEVM
                     NDIAVVPLVQRANIEVYRGTLMNRRLSNGIASQWWNIAQWYFK"
     gene            692..1882
                     /locus_tag="VFH60_02375"
     CDS             692..1882
                     /locus_tag="VFH60_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET6312654.1"
                     /translation="MTDKIVVEKLEQAVGIMRELDIDLWITYARETTLTPDPCLEIIA
                     GFEVTWHSAFLVSKTGERVAIVGRFDAENVQMTGGYSRVIPYDESIKPAMLEVVRGFD
                     PRSIALNYSENDPAADGLTHGMFLDLQRTFEGTPFASRFVSSEGLVSALRGRKSPSEV
                     ALIRAAIKTTEELYSQVGQGLGLGQKELAIAERLTSMRKELGLGTAWAEEYCPIVNAG
                     PDSPVGHAAPGQHETRPGHLLHMDFGVKQDGFCSDLQRMWYIPNEGEADIPDDVRRAW
                     DACWGAIDAGAALLKPGVRGWEVDAAARSYLVGAGYPEYQHALGHGLGRVAHDGATLL
                     GPRWDRYGNTPYGVVEAGNVFTLELGTVVPGRGYIGLEEDVLVTEEGLEWLSTPQREL
                     WVLR"
     gene            complement(1896..2411)
                     /locus_tag="VFH60_02380"
     CDS             complement(1896..2411)
                     /locus_tag="VFH60_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312655.1"
                     /translation="MSYCDNCGQARGAAKNYCRGCGEPLNLTSYTGYVEIANLSEGVA
                     TGIETAHRRMRWRKSTAEASNESLELYLKLVVIIALFHGVAYMGGSSILSLLNGNLGL
                     VLYGGVFLGAVFKMIFDVRLGYSLGRSLFEVLLIGVFAYGCVFGIFWWLTETYIHGGK
                     PLFDFSSLKNR"
     gene            complement(2780..3424)
                     /locus_tag="VFH60_02385"
     CDS             complement(2780..3424)
                     /locus_tag="VFH60_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312656.1"
                     /translation="MTFKNHSQGNIKRAGLITTLAGIMLVSLAACGGPAAPAATPVAT
                     ATQPAAMAPVETTVPEGLETAITTAPSAVATESTGMGDMGDMTEMGDEATATQAPADA
                     GGAATPTPAADQGASEGSTTQVQGTLREWAIDLSQQEVPAGKVIFTVTNTGQNTHNFA
                     VEDSTGAMIAKTPNFGSRDGAQTLELDLAPGTYTIICTLPGHATRGQRMQLVVK"
BASE COUNT          727 a         1080 c         1176 g          711 t
ORIGIN      
        1 cggcaaaata ccagttgccc gtgtataggt acgacccggc tgccgccaac cgcttacttg
       61 acgaggcggg ctgggcgagg ggagcggacg gcatccgtgt caaagatgga gagcggctgt
      121 cgttcgagta cggcacgacc cgcaacgcca ctcggcaggc tatccaggca ctcgtgcagg
      181 ccgacctcaa ggttgtgggt gttgaggcta taacggtgaa ctacccgcag ggcttctttg
      241 cccaagacgg acccgtggtt acaggcaaga ctaagctggc gcagtttgcc ttcgtgaacg
      301 acgaacttac cggcttcagc catttcagca acgacccggt gctgtccaac gtggagccta
      361 cgacgcgtaa ctaccagcaa tacaggaacg acagggtaac ggaggccaac cgcatcttca
      421 actcggagat agatcgtgga aagaccgccg aggccgccgc catcatccag gtggaggtga
      481 tgaacgacat agcggtggtg ccgctggtgc agcgcgccaa cattgaggtt tacaggggca
      541 cgctgatgaa ccgcaggctc agtaacggca ttgcttccca gtggtggaac atcgcgcagt
      601 ggtacttcaa gtagcggccc cgtgacccat tcggcaacgc tcctccctcg cctgtataat
      661 gcactgcata taagtttcag ggaggaagcg gatgacggac aagatagtgg tggaaaaact
      721 ggagcaggcg gtcggcatca tgcgcgagct ggacatcgac ctgtggatca cctacgcgcg
      781 cgagacgacc ctcacgcccg acccgtgcct ggagatcatc gcgggcttcg aggttacctg
      841 gcactcggct ttcctggtga gcaagaccgg cgagcgggtg gcgatagtgg gccggttcga
      901 tgccgagaac gtgcagatga cgggcggcta ctcgcgggtt ataccctatg acgagagcat
      961 caagcccgcc atgctggagg tggtgcgggg tttcgacccg cgcagcatcg cgctgaacta
     1021 ctccgagaac gatcccgccg ccgacggcct cacccacggc atgttcctcg acctgcaacg
     1081 caccttcgag ggcacgccct tcgcctcgcg gttcgtgtcg tccgaggggc tggtgtcggc
     1141 gctcaggggg cgtaagtcgc cctccgaggt ggccctcatc cgcgcggcca tcaagacgac
     1201 cgaagagctt tacagccagg tagggcaggg cctcggcctg gggcagaagg agctagccat
     1261 cgcggagcgg ctgacctcca tgcgcaagga gttggggctg ggcaccgcct gggccgagga
     1321 gtactgcccc atcgtgaacg cagggcctga ctcgccggtg ggccacgccg cccccggcca
     1381 gcatgagacc cggcccggcc acctgctgca catggacttc ggggtgaagc aggacggctt
     1441 ctgctccgac ctacaacgca tgtggtacat ccccaacgag ggcgaggcgg acatcccgga
     1501 cgacgtccgc cgggcttggg acgcctgctg gggcgctatc gacgcggggg ctgcgttgct
     1561 caagcctggc gtgaggggct gggaggtaga cgccgccgcc cgctcttacc tcgtcggggc
     1621 gggctacccg gagtaccagc acgcgctcgg acacggcctg ggtagggtgg cccacgacgg
     1681 cgcgacgctg ctggggccgc gctgggaccg ctacggcaac actccctacg gcgtggtgga
     1741 ggcgggcaac gtctttacgc tggagcttgg caccgtcgtg cccggcaggg gctacatcgg
     1801 ccttgaggaa gacgtactgg tcacggagga agggcttgag tggctgagca cgccgcaacg
     1861 ggagctttgg gtgctgaggt aggggcaggc gaaacttacc ggtttttcag gctgctaaaa
     1921 tcgaacagcg gcttgcctcc gtgtatgtag gtctccgtga gccaccagaa gatgccgaag
     1981 acacacccgt aagcgaacac tccgataagt aaaacctcaa acagactcct gcccaggcta
     2041 tagccgagcc gcacgtcgaa tatcatcttg aaaacggcac ccagaaatac cccgccgtac
     2101 aggacaaggc ccaggttgcc gtttagaagg ctcaggatcg aactgccgcc catataagcg
     2161 acgccgtgga aaagggcgat aatcacgact aacttcaagt aaagctccaa gctttcgttt
     2221 gaagcctcag ctgtgctctt tcgccaccgc atacgccggt gggccgtctc aataccggta
     2281 gctacaccct cgctgagatt ggcgatttca acataaccgg tgtaggaagt caggttgagt
     2341 ggttccccgc agccccggca gtagttcttt gccgccccgc gggcttgtcc gcagttgtcg
     2401 cagtaagaca tagtgacctt ttcctggcat gtgattaagg caggcgcgta agagttatcc
     2461 gcttgcctcc taccgagtat agtctcgcct gtatgcgtga gcaataggta aatagggtga
     2521 cttgttaagt cctaatgtac caaccgtggt cctagacagt tcatgcagag taacgaatac
     2581 agccaatcac ggtgccgggc gtctgctgtg tcacagttcc gggtaatggc ccagaactgt
     2641 gcaactctgc aagggcgctc atgaacggcg tacaaacaaa aagggagccg ttgcaggctc
     2701 ccctcggtaa ctatcgtgtc aaattgtcgc ttacgtgccg gaggtaccta acggggctgc
     2761 ctccgcgttg tccctacgcc tacttcacca ccaactgcat ccgttggccc ctggtagcgt
     2821 ggccgggcag ggtgcaaatg atggtgtaag tgccgggggc gaggtccagt tccagggttt
     2881 gagcgccgtc gcggctgccg aagttgggtg tcttggcaat catcgcgccg gtactgtcct
     2941 ctaccgcgaa gttatgggtg ttctgtcccg tgttagtcac cgtgaagatc accttgcctg
     3001 cgggtacttc ctgctggctc aggtcgatgg cccactcccg gagggtgccc tgcacctggg
     3061 ttgtgctgcc ctcgctcgcg ccctggtcgg cagccggggt gggcgtggct gctccaccgg
     3121 catcggcggg tgcctgcgtg gcggtcgcct catcccccat ctcggtcata tctcccatat
     3181 cgcccatgcc ggtgctttcg gtagccacgg cggagggggc ggttgttatg gccgtctcca
     3241 acccttccgg aacggtcgtc tccaccgggg ccattgccgc cggctgggtg gcggtggcta
     3301 cgggtgtcgc ggcgggggcg gctggcccgc cacaggctgc gaggcttacc agcattatgc
     3361 ctgccagggt ggtaatcaat ccggcccttt tgatgttgcc ttgtgaatgg ttcttgaatg
     3421 tcatgttgaa cgcttacctc tcttggtcgt gtcaatgttg tagtgtgtcg tacaggctgc
     3481 ccaatgggag gcggtgcttg cctgcttaca gtctaagatg cgaaccttaa agaaaaccct
     3541 gagaaaagtt aagatttggt taaggcggca gaggtgaagg gtccgaaacc acaaagacgc
     3601 caagatgaag gctggtgtgt tccatgcaaa gggccagggt acgtgtggat gtaccctggc
     3661 cctccccaaa agaaaagttc ccacttaagg aata
//
LOCUS       DASRWZ010000159         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1178182, whole genome shotgun sequence.
ACCESSION   DASRWZ010000159 DASRWZ010000000
VERSION     DASRWZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178182"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1043
                     /locus_tag="VFH60_02390"
     CDS             3..1043
                     /locus_tag="VFH60_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6312657.1"
                     /translation="MHRLAYGAKNLLTNNSSPFTRHASLATPEIAFEHVTKRFPSAAT
                     PAVDDVSLDVQQGDLVVLLGPSGCGKTTLLKMVNRLYEHDSGRILMQGREIRSFKVTD
                     LRRRIGYVIQSVGLFPHMTVARNIATVPEMLGWDKARINSRVDELLELVGLDPDEYRG
                     RYPAQLSGGQQQRVGLARAMSADPAIMLMDEPFAAIDSITRRRLQDELLNIQQKVRKT
                     ILFVTHDVEEAIRLADKIAVMRAGKVVQYDTPLRILSTPADDFVTELTGAGDVLRRLS
                     LLNVGLAMLPANGASPGPGAATTLHPRDSLRDALGQLLGSGAEVLPVVDEQGHHVGTI
                     DMAAIRRLTHPE"
     gene            1048..1731
                     /locus_tag="VFH60_02395"
     CDS             1048..1731
                     /locus_tag="VFH60_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6312658.1"
                     /translation="MDQLTRYLFANFGDKVWPRMVEHITLSGLALLIALLIALPLGLA
                     ISKVKPAAAPVLGVLDIVYTIPSFALFAFLVPITGIGTQPALIALTAYALVVLVHNTM
                     VAFNGVDPAVREAARGMGMSPVQVLWRIEVPLALPIIIAGIRIAALSTVGLTTIAAWI
                     GAGGMGQLLRDGMNDPTYSKLYAGIIGIGLIAVAADIVFRLLERLAGVPTHQKARKSP
                     SKDLLVKRA"
     gene            2130..>2720
                     /locus_tag="VFH60_02400"
     CDS             2130..>2720
                     /locus_tag="VFH60_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6312659.1"
                     /translation="MNPRNGLVDKLSAHVLLSGAALLLAVVIGMSLGIWISRFGALAR
                     VTVNFAGVLRVVPSIAVLFLLLPIVGTGFRPSLIALTFLAVPPILINTDAGMRSVSPA
                     IIEAGRGLGMSRWRLLWRVQLPLAIPVVLAGLRRAAVEVIASATLATLIGGGGLGDYI
                     QAGLALSRNDILLVGAVPVALLALVTELGLNYAERAF"
BASE COUNT          517 a          898 c          835 g          470 t
ORIGIN      
        1 ggatgcacag gctagcatat ggagcgaaga acttgctaac taacaactcg tcacccttca
       61 ctcgtcacgc gtcactcgct actcccgaaa tcgctttcga gcacgtgacc aagcggttcc
      121 cctcggccgc tacacccgcg gtggacgatg ttagcctcga cgtgcaacag ggcgatctgg
      181 tggtcttgct ggggccttcg ggctgcggca agaccacttt gcttaagatg gtgaaccgcc
      241 tgtacgagca cgactccggg cgcatcctca tgcagggccg ggagattcgg agcttcaagg
      301 tgacggacct gcggcggcgc atcggctacg tgatacagag cgtgggactc ttcccccaca
      361 tgacggtggc ccgcaatatc gcgaccgtgc cggagatgct gggctgggac aaggcgcgca
      421 tcaacagccg ggtggacgag ctactggagc tagtgggcct ggacccggac gagtaccggg
      481 gccgctatcc cgcgcagttg tcgggcggcc agcagcaacg cgtgggcctc gccagggcca
      541 tgtccgccga ccccgctata atgctcatgg acgagccgtt cgccgcgata gacagcatca
      601 cccgccgccg cctccaggac gagttgctca acatccagca gaaggtccgc aagaccatcc
      661 tcttcgtcac ccacgacgta gaggaggcga tacgactggc cgacaaaata gccgtcatgc
      721 gcgccggcaa ggtggtgcag tacgacacgc ccctgcgcat cctatcgacc cccgccgacg
      781 acttcgtgac cgaactcacc ggcgcgggcg acgtgctgcg acggctcagc ctcctcaacg
      841 tgggtttggc tatgctcccg gcaaacggag caagccccgg ccctggcgct gccactacgc
      901 tgcaccctcg tgatagcctg cgggatgccc tggggcagct tctcggcagc ggcgcggaag
      961 tcctccccgt tgtggacgag caggggcacc acgtgggcac catagatatg gccgcgatac
     1021 ggcgcttgac gcaccccgag tagaagcatg gaccaactga cccgctacct gttcgcgaac
     1081 ttcggtgaca aggtgtggcc ccgcatggtg gagcacatta cgctcagcgg cctcgccctg
     1141 cttatcgcac tgcttatcgc cttgccgctc ggactggcta ttagcaaggt caagcccgcc
     1201 gccgcgcccg tgctgggtgt gctcgacatc gtgtacacca tccccagctt cgccctgttc
     1261 gccttcctgg tgcccatcac cggcataggc acccagcccg ccctcatcgc cctgacggcc
     1321 tacgcgctgg tggtgctggt gcacaacacg atggtggcct tcaacggggt ggacccggca
     1381 gtgagggagg cggcaagggg catggggatg agtcccgtgc aggtgctctg gcgcatcgag
     1441 gtgccgctgg ccctccccat catcattgcg ggcatacgca tcgccgccct ctccacggtg
     1501 ggcctcacca ctatagccgc ctggataggc gcgggcggca tgggccagct actgcgcgac
     1561 ggcatgaacg accccaccta ctccaagctc tacgccggca tcatcggcat aggcctaatc
     1621 gccgtagccg ccgacatcgt cttccgcctc ctcgaacgcc tcgctggcgt gcccacccac
     1681 caaaaagccc gcaagtcccc ctcaaaggac ctgctggtga aacgtgcgtg acggattgtt
     1741 agggcctagg aagagacgaa accgcaaagg cgcaaagagc gcaaaggacg cgtagagaat
     1801 agggttggtt ttggtacgcg actaggccct gttggcataa ggagaccagg agtggcccct
     1861 gtcatcctga gcgcagcgaa ggatctaaag aaggaggaga ggtaggctgt cgaatatggg
     1921 agaaaggtaa cggatgttca ggatattcgc aaaggcaaca gcttagatcc ttcgctacgc
     1981 tcaggatgac aggggcactt tggtcccctt acgccaacag agggcagcca cataccaaca
     2041 agagcaatcc gcccaactca agactcaaaa ctcataactc ataactcact ggccctatgg
     2101 acacactaca gaaagcctta gaatacgcct tgaaccccag gaatggcctg gtagacaagc
     2161 tatcggcgca cgtactgctg agcggggcgg cgctcttgct ggcagtggtt ataggcatgt
     2221 cgctgggcat ctggatcagc cggtttgggg cgctggcgcg ggtcacggtc aacttcgcgg
     2281 gcgtgctccg cgtggtgcca agcatcgccg tgctttttct gctgctgccc atcgtgggca
     2341 ccggcttccg gccctcgctg atcgccctca ccttcctcgc cgtgcccccc atcctcatca
     2401 acaccgacgc gggcatgcgc agcgtcagcc cggccataat cgaggctggg cgcggcctgg
     2461 ggatgtcaag gtggcggctg ctgtggcggg tgcaactgcc cctggcgata cccgtagtgc
     2521 tggccggcct gcgcagggct gcggtggagg taatcgccag cgccaccctc gcaaccctca
     2581 tcggcggcgg cgggctgggc gactacatac aggcgggcct cgccctctcc cgcaacgaca
     2641 tcctgctggt aggggccgtg cccgtggccc tcctggccct ggtcaccgaa ctcggcctga
     2701 actacgccga gcgagccttc
//
LOCUS       DASRWZ010000160         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_492929, whole genome shotgun sequence.
ACCESSION   DASRWZ010000160 DASRWZ010000000
VERSION     DASRWZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492929"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VFH60_02405"
     CDS             complement(<1..959)
                     /locus_tag="VFH60_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312660.1"
                     /translation="MSEYSRGNIHPKTYLTIALVACALAALWSILQASTATATATAAG
                     RMAYVGTNASGLPATADGSTCEAAWRVVPSPNQGSGSNYLTAISAVSPSDIWAVGYYT
                     STGQYRTLTEHWDGTQWSIVPSPDPGYNNYLQGVAAIAHNDVWAVGSYDYPSSGGRHV
                     LTMHWNGTQWSVVANPDPGGNYTNFLNAVDAVSSGDVWAVGFWRRYSPQGLVMRWNGT
                     AWVQAANPSSGSVESMSAVTALSSNDVWSVGYSSTGNSLIWHWDGTQWSAVTSPNVGI
                     LHGLDASAPDDVWAIGGNDILNWDGTQWSVVPAPSVGTLNAITA"
     gene            1140..>2897
                     /locus_tag="VFH60_02410"
     CDS             1140..>2897
                     /locus_tag="VFH60_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HET6312661.1"
                     /translation="MQESVTFGQWIKERRKRLDLTREEVARCASCSLATIEKLETGER
                     RPSRQLAELVAKCLGIAAGDMAAFLSFARGASPLPDFAETSPTKGGETLQVPRLAAGL
                     ISPPTAFVGREREVLEVRRRLLQDDVRLLTLTGPPGIGKTRLALEVAAGLSNGEFADG
                     IVFVPLATTTEPGLVPARIGQALGLRESGGRPVAEVLKEWLRDKRLLLVLDNFEQVTD
                     GGAHLGELLLSAPSLKALVTSRTLLHTYGEHSFPVPSMSLPDKESAHEVESLAGYEAI
                     NLFVQRAKAARHDFQLTADNANAVTEICRRLDGLPLAIELAAMRARILTPQLILERLG
                     NRLDLLVGGSSDLPARQRSLRGAIEWSYSTLDAGEQALFRYISVFVGGCTLEAVEAVS
                     SHVASRAEELQAEGGGTTSHPGITNTIEVLSSLAGKSLLQVSETGAEARFSMLETLRE
                     YAWQRLEGVERDAASTAHATYFLRLAEQSEVGLLAREQSAWLARLEREHDNVRAALAR
                     LLEMGEAETAARLAGALRRFWYLRGHISEGRSWLERVLETREKLPTVVQAKTLHGVGT
                     LAWSQGDNSTAGRLFGESLD"
BASE COUNT          535 a          890 c          953 g          519 t
ORIGIN      
        1 gctgttatcg cgttcaaagt gcccacgctc ggagcgggca cgacgctcca ctgcgtgccg
       61 tcccagttca gtatgtcgtt gccgccgata gcccatacgt cgtcgggggc ggaggcgtcc
      121 aagccgtgca aaatgcctac gttcgggctg gtcacggcgc tccactgggt cccatcccag
      181 tgccagataa gcgagttgcc ggtggaactg taccctacag accatacatc attcgaggat
      241 aaggctgtta cggcgctcat agactccacg gagccgctcg agggattggc ggcctgcacc
      301 catgccgtgc cgttccaccg catgaccaga ccctgcgggc tgtagcgccg ccagaagccc
      361 accgcccata cgtcgccgga tgagacggca tccaccgcgt tgagaaagtt ggtgtagttg
      421 cctccggggt cggggttagc caccacactc cactgcgtgc cgttccagtg catcgtcagc
      481 acgtggcggc cgccggaaga ggggtaatca tagctgccca ccgcccatac atcgttatga
      541 gcgatggcgg ccactccctg gagatagttg ttgtagccgg ggtccgggct ggggacgata
      601 ctccactgcg tgccgtccca gtgctcggtt agcgtcctgt actggcctgt gctcgtgtag
      661 tagcccaccg cccagatgtc gctaggggac accgctgata tggccgtcag gtaattacta
      721 ccggaaccct ggttggggct agggaccacg cgccaggctg cctcacacgt cgaaccatcg
      781 gccgtggcgg gcagaccgct tgcattcgtg ccgacatatg ccatgcgccc tgcggctgta
      841 gctgtagctg tagctgtcga agcctgtagt atgctccaca gcgcggcaag cgcgcaggcc
      901 acgagcgcta ttgtcaaata ggtcttcggg tgtatgttgc cccggctgta ttcgctcatc
      961 aagttgtcct catgccttct actggtttat ccgtgctgtt gacggtcgat cttctgaatc
     1021 ttgaagcctg gcccttcaaa tctattatag aatcgaatcc gctataatga gccgtacaag
     1081 aacgcccaca aaacggcccc agaaagccac cgtcagcggt atataccgtc acccatgcca
     1141 tgcaagaaag cgtaactttc ggccagtgga tcaaggagcg ccgcaagcga ctcgacctga
     1201 cgcgcgaaga ggtagccagg tgcgcgtcct gctcgttggc gacaatcgag aagcttgaga
     1261 cgggagagcg ccgcccctcc aggcaactgg cggagctggt ggcgaagtgc ctgggcatcg
     1321 ctgccggtga catggcggcc ttcctgagct ttgccagggg cgcttccccg ctgccggact
     1381 tcgctgaaac gtctcccaca aaagggggcg agacgctaca ggtcccccgg ctcgcggcag
     1441 gcctgatttc cccacccacc gcgtttgtag gccgcgagcg ggaggtcctg gaggtccgcc
     1501 gccggttgct ccaggacgac gtgcgcctcc tgacgctcac cggccctccc ggtatcggca
     1561 agacccggct ggcgctggag gttgcggccg gcctgagcaa cggcgagttc gcggacggca
     1621 tcgtcttcgt ccccctcgct acgacgaccg agccgggctt ggtccccgcg aggatcggcc
     1681 aggcgctggg gcttcgcgag agcggcggcc gcccggtagc ggaggtcctg aaggagtggc
     1741 tgagggacaa acgcttgctg ctggtgctcg acaatttcga gcaggtaacc gacggcggcg
     1801 cccacctggg agagcttctc ttgtcggccc ccagcctgaa agcgctcgtc accagccgca
     1861 cgctgctgca cacctacggc gagcacagct tccccgtgcc ctctatgtcc ctgcccgaca
     1921 aggagagcgc gcacgaagtg gaaagcctgg ccgggtatga agccataaat ctgttcgttc
     1981 agagggcaaa ggccgcccgc catgacttcc agcttaccgc ggacaacgca aatgccgtga
     2041 ccgagatttg ccgccgactg gatgggcttc ccctggctat agagctggcg gccatgcgcg
     2101 cccggatatt gaccccgcaa ctgatcctgg agcggctggg aaaccggctc gatttgctgg
     2161 tcggaggcag ctcggacctg ccggcgcggc aaaggtcgct gaggggcgcg atagaatgga
     2221 gctacagcac gctggacgcc ggcgagcagg cgttgttcag gtacatctcg gtgttcgtgg
     2281 gcgggtgcac gctcgaagcg gtagaggcag tttcaagcca cgtcgcgagc cgtgccgagg
     2341 agctacaggc ggagggcggc ggaaccacct cgcacccagg aattaccaac actatcgagg
     2401 tcttgtcctc tctcgccggc aagagtctac tacaggtaag tgagacagga gccgaggcgc
     2461 gcttttccat gttggagacg ctgagggagt acgcatggca gcggctggaa ggggtggaga
     2521 gggacgcggc cagcacagcc catgcgactt acttcctgcg tttggcggag caatcggagg
     2581 tagggctgct cgcacgggag cagagcgcgt ggctggcgcg tctggaaaga gagcacgaca
     2641 atgttcgggc cgccctcgct cgcctgctgg agatgggtga ggcggagacc gccgcacgcc
     2701 tggcaggagc tttgcggcgc ttctggtacc ttcgcgggca catcagcgaa gggcgaagct
     2761 ggctggaaag ggtgcttgag accagggaga agctgccaac tgttgtgcag gctaagacgt
     2821 tgcacggcgt gggcacgctg gcgtggagcc agggcgacaa tagcacggcg ggccgcttgt
     2881 tcggcgagag cctggac
//
LOCUS       DASRWZ010000161         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1178469, whole genome shotgun sequence.
ACCESSION   DASRWZ010000161 DASRWZ010000000
VERSION     DASRWZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178469"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFH60_02415"
     CDS             <1..436
                     /locus_tag="VFH60_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6312662.1"
                     /translation="RRRKADEGLSFEALRHAIKHCDLELMFGFYAEDAELSIVNASVT
                     QTSPSELRGKAEVARLLRATFGQKTSRRVEREVVDEEQVTFEEACEYPDGSRLLVKTT
                     LEVRDGNIVRQTDLVAEDARADATLPNRLLSSEQATEKEDYW"
     gene            436..1257
                     /locus_tag="VFH60_02420"
     CDS             436..1257
                     /locus_tag="VFH60_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312663.1"
                     /translation="MSQFTSAAKRHPLITFFVLTYAIGWGCIPFWTFMAGSPFIAALI
                     VSPLTQGWAGLRELGSRMIRWRVRWYWYVVAIGLPLAVLLITAALNVALGASAPSLAF
                     GSVGAILLVFAVRLVNPGDGALGEEPGWRGFALPGLQGSGLSPLVSTLILGVLVTGWH
                     VPILFLEEGGLQPSIIVGYLLGSFAVTFWYTWLFNRTGGSVLMTIVSHAAQGTITIGG
                     LWSAGADFAQANLLNGVVLSVVAIGLVVLDWQFWRGPAPVPATVQPAYVGGPRVR"
BASE COUNT          266 a          470 c          493 g          302 t
ORIGIN      
        1 gaggcggcgg aaggctgacg aagggctgag cttcgaggct ctgcgccacg ccattaagca
       61 ttgcgatctg gagctcatgt tcggcttcta cgccgaggac gccgagctaa gcatcgtcaa
      121 cgccagcgtt acgcaaacct cgccctccga gctgcgcggc aaggcggagg tagccaggct
      181 cctgcgagcg accttcggcc agaagacgtc tcgccgcgtt gagcgggagg tcgtcgacga
      241 ggagcaggtg acgtttgagg aggcgtgcga gtacccggac ggcagccgtc tgctggtcaa
      301 gacgacgctg gaggtgcgcg acggcaatat cgtgcgccag acggacttgg tagcagagga
      361 tgcacgggcg gacgcaactc tacccaatcg ccttctaagt tctgaacagg caaccgagaa
      421 ggaggattac tggtaatgtc acaatttacg agcgcggcga agcgccaccc gctcatcacg
      481 ttcttcgtcc tgacctacgc gatcgggtgg gggtgcatac cgttctggac cttcatggca
      541 ggctcgccgt tcatcgccgc cctcatcgtg agccccctaa cccagggctg ggcggggctg
      601 cgagaactcg gctcccggat gatccgctgg cgcgtgagat ggtactggta cgtggtcgcc
      661 ataggactcc cgcttgctgt cctcctgatc accgcagcac tcaacgtcgc actcggggcg
      721 agcgccccct cgctggcctt cggctccgtg ggcgccatcc tcctggtgtt cgcggtgcgc
      781 ctagtcaacc ccggagatgg cgcgctgggc gaggagccgg gctggcgagg cttcgccctc
      841 ccagggctgc aaggatccgg tctctcgccg ctggtgtcca ccctcatcct cggcgtgctt
      901 gtaaccggct ggcacgtgcc catcctcttc ctggaagagg gcggtttgca gccgtctatc
      961 atcgtgggct acctactggg atcgtttgcg gtgacgttct ggtacacgtg gttgttcaac
     1021 cgcaccggcg gcagcgtgct catgacgatc gtgtcccacg ccgcccaagg gaccatcacg
     1081 ataggagggc tctggtccgc cggtgcagac tttgcgcagg ccaacttgct caatggcgtg
     1141 gtgctgagcg tcgtggcgat agggttggtc gtcttggact ggcagttctg gcgtggccct
     1201 gcgcctgttc ctgcgacggt acagcccgcg tatgtgggcg gaccgcgagt gcgatgaggt
     1261 gtcactctca ctaccggaag cctggtttct gggaatttcc gctctcggca gctcgtgaat
     1321 tgagccaaca cgcacatcag gcgcaaagcc gcgtcatcct atacgtccga acgcctatta
     1381 aaggtttttg aattccccca actcacgggt tgcatccggc caccgacgcg atagggtcat
     1441 gcgcgaggca agggaagcta ttgggaaggg tggtcactac ccccatgcac cacgacgatt
     1501 cgaggccaac ccatctgttg atgggcttaa t
//
LOCUS       DASRWZ010000162         1685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_904343, whole genome shotgun sequence.
ACCESSION   DASRWZ010000162 DASRWZ010000000
VERSION     DASRWZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904343"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFH60_02425"
     CDS             <1..207
                     /locus_tag="VFH60_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312664.1"
                     /translation="AVFGPSDPARYGPWDPAGRSVVVAPAGTKSGAESLRKAAAGGDK
                     YHDLVPADEVWGAVQKVFRPGRIG"
     gene            219..770
                     /locus_tag="VFH60_02430"
     CDS             219..770
                     /locus_tag="VFH60_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HET6312665.1"
                     /translation="MFDTGSTAPTGQTAIVIVPPPDIRGFADHYRQKYAAEQIWRIEA
                     HITVDYPFVPFDRIEEAEDRLRQLFAKCPPRAVAIRGFGIFPEDKVLYLRLAKPERVT
                     SLYEAVLAEFPQYPAYGRRPEEFVPHMTVGLFSDAEELERVYNELAPLRLFIAWDVES
                     VYLIYKTADGTWHPWSEIPLGEG"
     gene            785..>1685
                     /locus_tag="VFH60_02435"
     CDS             785..>1685
                     /locus_tag="VFH60_02435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET6312666.1"
                     /translation="MDDPKMLARELMVRIFCTPHALRNRLARRDGDTKHIKRILVMRP
                     DHLGDLLFATPTLDHIRQAFPTAYITGVVGPWGLAMWEGNPNLDALQVVPFPGIVERV
                     GGAEPYRLLARSAEQIAEGRYDLGVTLRFDHWWGAALMWAAGVPRRWGYDTPGMRSWL
                     TGRVPYTPGRHEVEQDLRLAHATIKAVGTSYTPRLEIDRAQGRPPLTPPNVSAPPLDL
                     LASWLSADAEKRVVIHPGTAGANKLWTIKGWAEVADRLAADGCSVLITGAPNEQTLCA
                     RIVQATSSKPLDLSGRTASISELA"
BASE COUNT          296 a          573 c          564 g          252 t
ORIGIN      
        1 gcggtgttcg gccccagcga cccggcgcgg tacgggcctt gggaccctgc ggggcggagc
       61 gtggtggtcg cgcccgcggg cacgaagtcg ggggccgagt ctttgcgcaa agcggcggcc
      121 gggggcgaca agtaccacga tctggtaccg gctgacgagg tatggggcgc tgtgcagaag
      181 gtgttcaggc cggggcgcat cgggtaagat aaagtactat gttcgacacc ggctcaacag
      241 ctcccaccgg gcaaacggct atcgttatcg tgccgccgcc cgacatacgg ggcttcgccg
      301 accactaccg ccagaagtac gccgccgagc aaatctggcg catcgaggcc cacatcacgg
      361 tggactaccc gttcgtgccc ttcgaccgca tcgaggaggc cgaggatagg ctgaggcaac
      421 tgttcgccaa atgcccgccc agggcggtcg cgatacgcgg cttcggcatc ttcccggagg
      481 acaaggtgct ctacctgcgc ctggccaagc cggagcgggt cacgtccctc tacgaggcgg
      541 tgcttgccga gttcccccag tacccggcgt atggcaggcg gcccgaagag ttcgtgcccc
      601 acatgacggt gggcctcttc tccgacgcgg aggagcttga gcgcgtctac aacgagcttg
      661 cgcccctgcg ccttttcatc gcgtgggacg tggaaagcgt gtacctcatc tacaagacgg
      721 cggacggcac ctggcacccc tggtccgaga tcccgctggg cgagggctga cacgcgtggc
      781 gggcatggac gacccgaaga tgctggcgcg cgagctgatg gtgcgcatct tctgcacgcc
      841 ccacgccctg cgaaacaggc tggcccgccg cgacggcgac accaaacaca taaaacgcat
      901 cctcgttatg cggcccgacc acctgggcga cctgcttttt gcgaccccta ccctggacca
      961 catccggcaa gccttcccca ccgcctacat aacgggagtg gtggggccgt ggggcctcgc
     1021 catgtgggag ggcaacccca acctggacgc gctacaggtg gtgccctttc ccggcatcgt
     1081 ggagcgggtg ggcggcgcgg agccgtacag gctgctcgcc cgctcggcgg agcagatagc
     1141 cgagggccgc tacgacctgg gcgtgaccct gcgcttcgac cactggtggg gcgcggccct
     1201 catgtgggcg gcgggcgtgc ccaggcggtg gggctacgac acgcccggca tgcgaagctg
     1261 gctcaccggc cgggtgccct acacgccagg caggcacgag gtcgagcaag atttgcggct
     1321 ggcacacgct actatcaaag ccgtaggcac cagttacacg ccacggctgg aaatagaccg
     1381 cgcgcaaggc cgaccccccc tcaccccgcc caacgttagc gcacctccgc tggacctgct
     1441 cgcctcctgg ctgagcgcgg acgcggagaa acgggtggta atccaccccg gcacggcagg
     1501 ggctaacaag ctctggacca tcaagggctg ggccgaggta gcggacaggc tggcggcaga
     1561 cgggtgcagc gtcctcatca ccggggcacc caacgagcaa accctgtgcg ctcgtattgt
     1621 tcaggccacc tcaagtaagc ccctcgacct gtcgggccgc accgccagta tctccgaact
     1681 ggcct
//
LOCUS       DASRWZ010000163         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_561980, whole genome shotgun sequence.
ACCESSION   DASRWZ010000163 DASRWZ010000000
VERSION     DASRWZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561980"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1433)
                     /locus_tag="VFH60_02440"
     CDS             complement(225..1433)
                     /locus_tag="VFH60_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6312667.1"
                     /translation="MSKLLRIVGLLMLLEKAWRHLLVVRFFRRPVPRATREVELVSIL
                     QPILSGDPTMPACLERSLRLRSRYPLEFIWLVDVDDPHAQRICMDLCRKYPDREVNVV
                     VMPPPAERQNPKTVKLIAGARLANGDVLCVLDDDTQLPDGGLEKCLPYLDRPGVGLAF
                     GLPYYLEFSNLWSSLVSYFVDSHSLLTYIPYTALSEPFTINGMFYAARRDVLESVGGF
                     EGLEKMLADDFAVAQRFRRNGYRLAQTPLLHGISTRVTGPRHYLSLIQRWFIFPRESL
                     MRHLKPREQAVLYGLGLVPSLFPLSLLGYLLGRPSKGKALYSLLYFGYSYAVFAHINK
                     RYLGGAAAWGKSWLVPVIQVVFPLQLLAALLSPQRITWRGHEMQVERGGGFRFVRRRG
                     TALTGRTQNP"
     gene            complement(1430..2611)
                     /locus_tag="VFH60_02445"
     CDS             complement(1430..2611)
                     /locus_tag="VFH60_02445"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6312668.1"
                     /translation="MSRVADKLAQGLLAAFAIDRALKLAAVVHFFQRKPPRPPSPWPT
                     VTLLQPVSWGASDLRAALLSRARLDYPVRVQHLFVCDEADAESRAACGALFEDFPGLC
                     GQVVGVPAGASPMSFKTVKLQAGMAYATGDVVCCVDDDIILRPNALTTLVPYLYERGA
                     GAAFGLACYTSWRNLPTSLMSAFVNANALLSYVPLTYMAEPFTITGHCYALRREVLQS
                     VDEFRGMEHHFGDDHELAWRLRAKGLRSVQTPLVYDVVNHFDTLGQYAVQMKRWFVFP
                     RQMLLPAMTQREQAVSFVGSAGNLVPGLLLLLALITRRPAAGRALGWSLGLSAAIYAW
                     CEALYLKRRTPLKGWPALLAATLFAPFQVVWALLSDDVIEWRGRRVRVLKGGRYEEVE
                     A"
     gene            complement(2608..3468)
                     /locus_tag="VFH60_02450"
     CDS             complement(2608..3468)
                     /locus_tag="VFH60_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6312669.1"
                     /translation="MPQDPFRQYKQFLATHSFRHLDVEGSRWPYIVGGSGESALVLLP
                     GAPGRPEASFEYVLAFEREYTVISVGYPAEVATVEECLRGLASIMEAEGFGRAHVVGG
                     SYSGLLAQRFVRQYPGKVDRLVLSDTGVPRPGRARKYRRYVWLLRVLPMPVVRALWRV
                     GAYLYLRDMAPASRPFWRAYFALVRRSITRRECISRLQVWVDFDSGSRFSPGDLATWP
                     GEVLILEAERDTTFPAWERQALRKLYPTAEVRVFTGGGHAASLDRRDEYIAAIRAFLA
                     AHPSQREAAS"
BASE COUNT          712 a         1198 c         1183 g          552 t
ORIGIN      
        1 ctgccacata ccgtcttcgc cgtttcggtg aagtatctac aatcgtcctg cgttttagcg
       61 cctgctggga ttgatgctgg aatcgtggaa gcaactgaga acatgcctac aagaagagca
      121 agaaggcaag ggtaaggtct atgattaccg gagttggtcc ttaacatgac acgccccttt
      181 acttcccttg atcgtgttga ggagttcacc agagaggcca aagctcaggg attctgggtc
      241 cgtcctgtca aggctgtgcc acgcctgcga acgaagcgga acccaccgcc ccgctccacc
      301 tgcatctcat gccctcgcca cgtaatgcgc tgcggagaga gcagggccgc caggagctgc
      361 aacggaaata ccacctgtat cacgggcacc agccacgatt taccccaggc cgccgcccct
      421 ccaaggtagc gcttgttgat gtgggcgaac accgcgtagc tgtagccaaa gtacagcagc
      481 gagtacaacg ccttcccctt cgagggccgc cccagcaggt aacccaacag cgatagcggg
      541 aacagcgacg gcaccagccc caggccgtaa agcaccgcct gctccctcgg cttgaggtgc
      601 cgcatgagcg actcgcgggg gaagatgaac caccgctgga tgaggctgag gtaatgtcgt
      661 gggccggtga cgcgcgtgct gatgccgtgc aggaggggcg tctgtgccag gcggtagcca
      721 ttgcgcctga agcgttgcgc caccgcgaaa tcgtccgcca gcatcttctc cagcccctcg
      781 aagccgccca ccgactccag tacgtcgcgg cgcgcggcgt agaacatgcc gttgatagta
      841 aacggctcgg acagggcggt gtaggggatg taggtaagca ggctatggct gtccacgaag
      901 taagagacca gggaggacca gaggttggag aattcgagat agtaaggcag gccgaacgcc
      961 aaccccactc ccggccggtc gaggtagggc aggcactttt cgaggccgcc gtcgggtagc
     1021 tgcgtgtcgt cgtccagcac gcagagcacg tcgccgttag ccagccgggc acccgcaatg
     1081 agcttgaccg tcttggggtt ctgccgctcg gcgggcgggg gcatgaccac cacgtttacc
     1141 tcgcggtcgg ggtacttgcg gcaaaggtcc atgcagatgc gctgggcgtg ggggtcgtca
     1201 acgtccacca gccagatgaa ctccaacggg tagcggctgc gcaggcggag gctgcgctcg
     1261 aggcaagcag gcatggtggg gtcgccgctc aggatgggct ggagaatgct gaccagctcc
     1321 acttcgcggg tcgccctcgg caccggcctg cgaaagaagc gcaccaccaa taggtgccgc
     1381 caggctttct ccaacagcat caacagcccc acaatccgaa gcagcttgct caagcctcga
     1441 cctcctcgta tcgcccgccc ttcagcacgc gcactcgcct gccgcgccac tcgatcacgt
     1501 cgtccgacag gagcgcccac accacctgga acggggcgaa cagcgtggca gccagaagcg
     1561 cgggccaccc cttcagcggc gtgcgcctct tgaggtacag cgcctcgcac caggcgtaaa
     1621 tcgcggcgga tagccccagg ctccagccaa gcgcgcgccc ggcggcgggc ctgcgggtaa
     1681 tgagcgccag caacaggagc agaccgggca ccaggttgcc cgcgctaccg acgaagctca
     1741 ccgcctgctc gcgttgggtc atggcgggca aaagcatctg gcgcgggaag acgaaccagc
     1801 gcttcatctg cacggcgtac tggcccagcg tgtcgaagtg gttcaccacg tcgtagacga
     1861 gcggagtctg caccgagcgc aggcccttcg cccgcaagcg ccaggccagc tcgtggtcgt
     1921 cgccaaagtg gtgctccatg ccccggaact cgtccaccga ctgaagcact tcacggcgca
     1981 gagcgtagca atgcccggtg atcgtgaaag gctcggccat gtaggtgagc ggcacgtagc
     2041 tgagcagggc gttggcgttg acaaaggcgc tcatcaggct cgtgggcagg ttgcgccagc
     2101 tcgtgtagca ggcgagaccg aacgccgccc cggccccccg ctcgtaaagg tagggcacca
     2161 gcgtggtcag cgcgttgggc cgcaaaatta tgtcgtcgtc cacacagcac acgacatctc
     2221 ccgttgcata ggccatgccc gcctgcaact tgaccgtctt gaacgacatg ggagacgccc
     2281 ccgcaggcac gcccactact tgcccgcaca ggccggggaa atcctcgaac aacgccccgc
     2341 acgccgcccg cgattccgcg tcggcctcgt cgcacacgaa caggtgctgc acccgcaccg
     2401 ggtagtcgag cctggcacgc gacaggaggg ccgcacgcag gtcgctggcc ccccacgaca
     2461 cgggctgaag cagcgtcacg gtgggccacg gagagggcgg gcgcgggggt ttacgctgga
     2521 agaaatggac tacggcagcg agttttaagg cccggtcgat ggcgaaggcc gccaggagac
     2581 cttgtgcaag cttgtcagca acccggctca agacgcggcc tcacgctgag aagggtgggc
     2641 ggcgaggaag gcccggatgg ctgcgatgta ctcgtcccgc cggtcgagcg aggccgcgtg
     2701 cccgcccccg gtgaagaccc gcacctcggc agtgggatag agcttccgca gggcctgccg
     2761 ctcccacgcg gggaatgtgg tgtcgcgctc ggcctccagg atgagcacct cgccgggcca
     2821 ggtcgcaagg tcgccggggg agaagcggct gccgctgtcg aaatcgaccc acacttgcaa
     2881 gcgactgatg cactcgcggc gggtgatcga cctcctcacc agcgcgaagt acgcccgcca
     2941 gaaggggcgg ctggccgggg ccatatcgcg cagatagagg tacgcaccca cgcgccagag
     3001 cgcccgcacc acgggcatgg gcagcacgcg caaaagccac acgtagcgcc ggtacttacg
     3061 ggcgcgcccc ggcctgggca cccccgtgtc cgacagtacc agccggtcca ctttgccggg
     3121 gtactgccgc acgaaccgct gggccagcag cccgctgtag gagccgccga ccacgtgcgc
     3181 ccgcccaaag ccttccgcct ccattatgct cgccaggccc cgcaagcact cttcaaccgt
     3241 ggcgacctcc gccgggtagc caaccgagat gaccgtgtac tcccgctcga aggccagcac
     3301 gtactcaaac gaggcctcgg gacgtccggg ggcaccgggc aataacacca atgcactctc
     3361 accgctccca ccgacgatgt agggccagcg cgagccctct acgtccaagt gtcggaacga
     3421 gtgggtagcg aggaactgct tgtattgtcg aaaggggtct tggggcatgt tagctggtga
     3481 gcgcctgtgg ttccggcgcg cttcccagtg taccttaagc gcgcttgtgg agagtagggc
     3541 gcacagcacc caaaggtggc cctgtcatcc tgagtgtagc gaaggatcag gtggcagccg
     3601 gtctctgtgg caatgcgtga ggctggcggc aagccaacct gggta
//
LOCUS       DASRWZ010000164         3963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_972766, whole genome shotgun sequence.
ACCESSION   DASRWZ010000164 DASRWZ010000000
VERSION     DASRWZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972766"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFH60_02455"
     CDS             <1..597
                     /locus_tag="VFH60_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009226263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6312670.1"
                     /translation="MGLLPAQEGEVRWNGQLVENPATFFVPPRAAYTPQVPRLFSETL
                     RDNILLGLTEDHSDLPGALRMAVLERDIFDLEQGLGTHVGPRGVKLSGGQIQRSAAAR
                     MFVRDAELLVMDDLSSALDVETERILWDGLFRQRDDATCLAVSHRRAALTRADHIIVL
                     KDGKVEAQGTLGHLLATSEEMKALWQGEAEAEGAAVGV"
     gene            1015..1272
                     /locus_tag="VFH60_02460"
     CDS             1015..1272
                     /locus_tag="VFH60_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312671.1"
                     /translation="MDKPWFDESGMLLLDDYVMQSASYQRVTDDHQVEEEEIAAQAQR
                     VAELLKQLEGLLSPEAKAVATDALSELAVLSLLQAKRAGGG"
     gene            1324..2190
                     /locus_tag="VFH60_02465"
     CDS             1324..2190
                     /locus_tag="VFH60_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6312672.1"
                     /translation="MPTFESVKTVPVRLADMAPAASEVMNYFRAQGYEVTGKPTGTGG
                     VLISLRKGDTFKAVLGMKTSLNIEIDPAIDGTLIRAKVGIWGEQAVPTMIMLFVAWPV
                     LLTQIWGMAQQANLDDEALFRTEQALLRAAGMPTVTEPTQAPAPGQAAAPYTPGPYVP
                     PPVYSQPQQQQQQQPQPQQQAFYTSQPAPQVAPAAAPVGYTYGQPPPEQPQQTGYSPT
                     PSPVAAATGPATPQEALAAAVPASAPPAQAPAPAGETASAPPSTIFCMSCGAQLPGDA
                     RFCFKCGKPVAS"
     gene            complement(2384..3613)
                     /locus_tag="VFH60_02470"
     CDS             complement(2384..3613)
                     /locus_tag="VFH60_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312673.1"
                     /translation="MNPDTLSDWLLPGLGIFVALVMLPTVVLIWHAKRQQRPKFVTQD
                     DFTDKFDKVMTSTNEATRLAREAFTTSVAALDATNLAAQDTRKALGTISSTITDQHDD
                     LKGTLVKAMEDSRAVNRAVVTAVQGLVDHTREELSVSRSTANDMAEAVKALKSISTDF
                     NQAVVTLRSATPEEGPYTDELSSAAITEDLSATQVGGRVGNHVQARVMKAPQTHLRDV
                     SLNRGPLDTLPGIGDEQSQIDDTTPLVGRLGRVEVEPADATLPSHAPQARPATVSSDV
                     MELLQLYAGAFADLVAAYQRSRPLNDREIVELAGTYCTTWISRLQSLHERRVPAEVWQ
                     RFYMHAIALQTVNDFRSRLQGSALRVAAELWERQARNAPGTHEVFRKIAAEIRRQVGE
                     VERQTQPPAEYAARRVS"
BASE COUNT          740 a         1223 c         1255 g          745 t
ORIGIN      
        1 atgggcctgc tgcccgccca ggagggggag gtacgctgga acgggcagtt ggtggagaac
       61 ccggccacct tcttcgtgcc gccacgcgcc gcctatacgc cacaggtgcc ccggctcttc
      121 agcgagacgt tgcgggacaa catcctgctc ggcctgacgg aagaccactc cgacctgcct
      181 ggggcgttac gcatggcggt gctggaacgc gacatcttcg acctggagca ggggttgggc
      241 acgcacgtcg gcccgcgcgg ggtgaagctg tcgggcgggc agatacagcg cagtgccgcc
      301 gcccgcatgt tcgtgcgcga cgccgagctg ctggtgatgg acgacctgtc gagcgcgctg
      361 gacgtggaga cggagcgcat actgtgggac ggcctgttcc gccagcgcga cgacgccacc
      421 tgcctggccg tctcgcaccg ccgggccgca ctcacccgcg ccgaccacat catcgtgctc
      481 aaggacggta aggtagaggc tcaaggcacc ctcggccact tgctggctac ctcggaggag
      541 atgaaggcgc tgtggcaggg agaggcggag gccgaggggg cagcggtggg ggtgtagtaa
      601 gttatgagtt atggtaggta catgctaacc tgctccaatg ttgggaccaa atcccgcctt
      661 tgtcattctg agcgtagcga agaatctcgt cctcaagcac ccaaatgacc cctttctatc
      721 cgctggtatt gaaaactgta acatcccggt ggaggacgag attcttcgct gcgctcagaa
      781 tgacaaggtc caccaacatc cctccctcgt accctgtgac attcacgcac tgcaaacggt
      841 aagccatatc actcagcgct cgtactcagc actcgtatta ctcagtcctc agtcctcagt
      901 cctcatcact catctaacac attgacgact ttccggcccg cttatataat gcctcagatt
      961 ggtaggtcat tatgaccttc cagcgacgct gcttacattc aggaggaggc ttgtatggat
     1021 aagccctggt ttgacgagtc gggcatgttg ctgctcgacg attacgttat gcaatcggct
     1081 tcctaccaga gggtcaccga tgaccaccag gttgaggaag aagagatagc cgcacaggcg
     1141 cagcgcgtgg cggagttgct gaagcaactg gaagggctgc tctcgccgga ggccaaggcc
     1201 gtcgctacgg acgccctgag cgagctagcg gtgctcagtt tgttgcaggc caagagggcc
     1261 ggaggtggct agtttcacag gtgcgggcgg catacaacac gattgataag aaggagaacg
     1321 tatatgccaa ctttcgaatc ggtcaagacg gtccccgtga ggctggcgga catggccccc
     1381 gccgcctcag aggtgatgaa ctacttccgg gcgcagggct acgaggtgac gggcaaaccc
     1441 accggcacgg gaggcgtgct tatcagcctg cgcaagggcg acactttcaa ggctgtactc
     1501 ggcatgaaga cctccctcaa tatagagatc gaccccgcta tagatggcac gctcattagg
     1561 gcgaaggtgg gcatctgggg cgagcaagcg gtgcccacga tgatcatgtt gttcgtggcc
     1621 tggcccgtgc tgctcacgca gatctggggc atggcgcagc aagccaacct ggacgacgaa
     1681 gcgctcttcc gtaccgagca ggcgctattg cgtgcggcgg ggatgcccac ggtgaccgag
     1741 cctacgcaag cccccgcgcc gggccaggca gccgcgccct acacccccgg tccttacgtc
     1801 ccgccgcccg tctattccca gccgcaacag caacagcaac agcagcccca accacaacag
     1861 caggcttttt acacctcgca gcccgctcct caggtcgctc ccgctgccgc gccggtcggc
     1921 tacacctacg gacagcctcc gccggagcag ccgcagcaga ccggctatag ccccacgcct
     1981 agcccagtag ctgccgccac aggcccggcc acgccccagg aagcccttgc cgccgcggtg
     2041 cccgccagcg cccccccagc tcaggccccg gcacccgccg gagagactgc cagcgcaccg
     2101 ccctcgacca tcttctgcat gagctgcggc gcgcaattac cgggagacgc ccgcttctgc
     2161 ttcaagtgcg gcaagccggt cgcctcatag cagcaggaga cgaaaacgca aagacgcaaa
     2221 ggacgcaaag gacgcgcaaa gagtagaaag gtttagaggg tacaaagagg accagggtac
     2281 ggaagatgta ccctggtcct cttgcttaga acacccacat cctaatctct gcgcgttctt
     2341 tgcgtctttg cgtctttgcg ttttcgtctc tttaccgtgt tgactacgat actcgcctgg
     2401 ctgcatactc ggcggggggt tgggtttgcc gctcgacctc gcccacctgt ctcctgattt
     2461 cggcggcgat cttgcggaag acctcgtggg tgcctggggc gttgcgggcc tggcgctccc
     2521 acaactcggc ggccacgcgc agggcggaac cttgcaggcg gctgcggaag tcatttaccg
     2581 tctggagcgc gatggcgtgc atgtagaacc gctgccagac ttcagccggt acgcgccgct
     2641 cgtgcagcga ttgcagcctc gaaatccagg tggtgcagta ggtgcccgca agctccacga
     2701 tctcgcggtc gttgagaggg cgcgaccgct ggtaggcggc aacgaggtct gcgaacgccc
     2761 ccgcgtacag ttgcagcagc tccatcacgt cgctggagac agtggcgggc ctggcctggg
     2821 gggcatggct gggtagggtt gcatcggccg gctcaacctc cactctgcca agcctgccaa
     2881 ccaggggggt agtatcgtct atctgggact gctcgtcccc aatgccgggc agggtgtcga
     2941 gcggcccgcg gttgagcgac acgtcgcgca ggtgggtctg gggtgccttc atcacgcggg
     3001 cctgtacgtg gttgcccacg cggccgccca cctgggtggc cgaaaggtct tccgtgatgg
     3061 cagcggaaga aagctcgtcg gtatacggcc cttcctccgg tgtggcgctg cgcagcgtga
     3121 ccaccgcctg gttgaagtcg gtcgagatag acttgagggc cttgaccgcc tcggccatat
     3181 cgttggcggt gcttctcgac acgctcagct cctcgcgggt gtggtccacc aggccctgga
     3241 cggccgtgac caccgcgcga ttgacggccc tgctatcctc catcgccttg acgagggtgc
     3301 ccttgagatc gtcgtgctgg tcggtgatgg tagagctgat cgtgccgagg gccttgcggg
     3361 tgtcctgggc ggccaggttg gtggcgtcga gggcggccac ggaggtggtg aaagcctccc
     3421 tcgccaggcg cgtggcctcg ttggtgctgg tcatcacctt gtcgaacttg tccgtgaagt
     3481 cgtcctgcgt gacgaactta ggtcgctgct gccgtttagc gtgccagatg agcacgacgg
     3541 tcggcagcat gacaagcgct acgaagatgc ctaagccggg tagcaaccaa tcgctcagag
     3601 tatcagggtt cacagctatg ccctctcacg tttagcattc ggcggggtcc gcgtcgtccc
     3661 ggcgacgtac cgggctgcga caggtgccca ttatatagca gcccgcaggc gaccgttgcg
     3721 gtgtccaccg tgaagcgggg gatgtggaaa actcgagacg gcgcctcctc ggcatgatag
     3781 gagcagtcgc atacagataa gcatgattgg agtccatgct agggtattca gcggcagctt
     3841 gcagcgagcg cttattatta gcctctcgtt gtgatgtagt agcaactttt ccgtgacacg
     3901 ccgcgtataa tatagtaggg gtgcactttc atagcgacgt ctatttacag gagaactgga
     3961 ctg
//
LOCUS       DASRWZ010000165         4105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1041532, whole genome shotgun sequence.
ACCESSION   DASRWZ010000165 DASRWZ010000000
VERSION     DASRWZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041532"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1264
                     /locus_tag="VFH60_02475"
     CDS             83..1264
                     /locus_tag="VFH60_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312674.1"
                     /translation="MADEKLVGILVGRENTFPPAFIDRVNSKNEGVRAEFCKLGGTRM
                     DEPPVYSVIVDRISHEIPYFRAYLKKAVLDGTVVINNPFWWSADDKFFECCLADRQGV
                     AVPRTVVLPQKSYVEGVVTESLRNLQYPMDWASLAEYVGFPAFMKPFVGGGWRNVYKV
                     HNVEELIWCFDQTGELGMILQEGIAFEKYARCIVLGQSTANIIRYEPGNPFHLRYSTD
                     DPVEFFGKELYERIYRDCITLTSSLGYDMDTVEFAVRDGVPYAIDWLNPACDMDYHSV
                     TPKNFEWCVENMANLVIDRAKNGSPQPGEYRWDRFLNAAPPPTEGPVKNWTEFAHEDV
                     AGAEQEKREAAAKSGGRAEAKSMPVPAAGNTSGVPEDVQSRGDGGPSIPHPVPNGETT
                     P"
     gene            1261..2691
                     /locus_tag="VFH60_02480"
     CDS             1261..2691
                     /locus_tag="VFH60_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312675.1"
                     /translation="MSGHGPSAAINAESAHPAIEDFHEFLAANPQVAADSQEMLARES
                     VTRLLTFGGEPFTRYLRPHMITPGQYGVITDVVKTLASAMQKLRRACLADAGMRDQLD
                     LTPEELRLAMIDPGFEEPSPSIRLDSFWSERDWRFVEINAESPAAIAYEDVLADLFLE
                     LPAINQWRYSNGYYLSPLYARTRFIQAIRQAWTEFKQTYGPCFNDNPTRAIVDWAGVP
                     TDTEFEIFRRYFEELGFKTLVTQPERLEFRDGRLRDGDFVIDIYYKRVLTSELLEKPD
                     VARATIQAYEAGKICVINSFRAKFLHKKMSLGLMHDDANAHLFSEHELDVIRRHIPWT
                     RKVAEGHTTLNGQSIDLLPFIADNRESLVLKPNDEYGGKGVVIGWTASDEEWQRAIKE
                     ALESSFVVQWAVQLDQEPYPYYDQQQGVSFRDLTADLDPFVFGPDMAGILTRLSAAAL
                     LNVTAGTGSVVPTMIVEKDPIPARAR"
     gene            2748..3869
                     /locus_tag="VFH60_02485"
     CDS             2748..3869
                     /locus_tag="VFH60_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HET6312676.1"
                     /translation="MQKFFTIGVEEEFQIVDPSTRDLKPFVEQMLSHGDEDFIEHVKR
                     EMIQSMVETVTPVCQNVEEARREVYRLRAGVAELAARNGLAIVASGAHPFSHWQSQII
                     TEHERYKVLEQDLQDVVRSILIFGLHVHVGIPDRDLLIDIMNEARYFLPHMLALSSNS
                     PFWQGRHTGLKSYRTIIWQQFPRTGIPSTLGSWSDFENFVNLLIKTNSIDNGKKIWWD
                     LRPHPYFSTLEFRVCDMPTNPAETIMIVAFFQAIVAKLYKLRQKNLGFRIYDRALSME
                     NKWRAARYGLDGMLIDFGKEQEVPMRQLALELLDFVDDVVDDLGSREAVNYIHQVLQQ
                     GTGADRQMEVFRRTGDLNDVVDYLIYETTNGLDWQGYGR"
BASE COUNT          863 a         1183 c         1348 g          711 t
ORIGIN      
        1 tgagttatga gttttgagtt attcagccaa ctcaaaactc ataactcaaa actcattact
       61 aaacagacca aggaggacga ggatggcaga cgagaaactg gtaggcatac ttgtggggcg
      121 agagaacacc tttcctcccg ccttcatcga ccgggtgaat tccaagaacg agggagtgcg
      181 ggccgagttc tgcaagctgg ggggcacgcg catggacgag ccgcctgtgt acagcgtgat
      241 cgtggaccgc atctcgcacg aaatccccta ttttcgcgcc tacctaaaga aggcggtgct
      301 cgatgggacg gtggtgatta acaacccgtt ctggtggagc gcggacgaca agttcttcga
      361 gtgctgcctt gccgacaggc agggggtggc cgtgcctcgt acggtggtgc tgccgcaaaa
      421 atcgtacgtg gagggggtgg tgacggagtc gctacgcaac ctccagtatc cgatggactg
      481 ggcgtccctc gcggagtacg tgggcttccc tgcgtttatg aagccgtttg taggtggagg
      541 gtggaggaac gtatacaagg tacataacgt cgaagagctt atctggtgct tcgaccagac
      601 aggcgagctg ggcatgatct tgcaagaggg catcgcgttc gagaagtacg cccgctgtat
      661 cgtgcttggg cagagcacgg caaacatcat tcgctacgag ccgggcaacc cgttccacct
      721 gcgctacagc accgacgacc cggtggagtt cttcggcaag gaactgtacg agcgcatcta
      781 ccgcgattgc attaccctca ccagcagcct gggctacgac atggacacgg tggagttcgc
      841 ggtgcgcgac ggcgtcccct acgcgataga ctggctcaac ccggcctgcg atatggacta
      901 ccactcggtt acccccaaga acttcgagtg gtgcgtggag aacatggcta acctggttat
      961 cgaccgggcc aagaacggct ctccacagcc gggcgagtac cgctgggacc gcttcctcaa
     1021 cgccgcgccc ccgcccaccg aagggccggt gaagaactgg accgaattcg cgcacgagga
     1081 cgtggcgggc gcggagcagg agaagcggga ggcggcagcg aagtcgggcg gcagggcgga
     1141 ggccaagtcg atgccggtgc ccgcggcggg caacacgtcc ggcgtaccgg aggacgtgca
     1201 aagccggggc gatggcgggc cgtcgatacc ccacccggtg ccgaacggag agaccacgcc
     1261 atgagcgggc acggcccgtc ggcggctatc aacgctgaga gcgcccaccc cgcgatagag
     1321 gacttccacg agttcctggc cgccaacccg caggtcgccg ccgactcgca ggagatgctg
     1381 gcgagggaga gcgtcacgag gctgctcacg ttcggcgggg agccgttcac gcgctacctg
     1441 cgcccgcaca tgataacgcc cggccagtac ggcgtcatta ccgacgtggt aaagacgctc
     1501 gcctcggcca tgcagaagtt gcggcgggcg tgcctggcgg acgctggcat gcgggaccaa
     1561 ctcgacctga cgccggaaga gctacggctc gcgatgatcg accccggctt cgaggagccg
     1621 tcgcccagca tccgactcga ctcgttctgg tcggagcggg actggcgctt cgtggagata
     1681 aacgccgagt ccccggccgc catcgcctat gaggacgtgc tggcggacct cttccttgaa
     1741 ctgcccgcga tcaaccagtg gcggtattcg aacggctatt atctaagccc gctctatgcg
     1801 cgcactcggt tcatacaggc catacgccag gcatggaccg agttcaaaca aacctacggg
     1861 ccgtgcttca acgacaaccc cacgagagcg attgtagact gggcgggggt gccgacggac
     1921 acggagttcg agattttcag gcggtacttt gaggagttgg gcttcaagac gctggtcact
     1981 cagccggaga ggttggagtt ccgcgacggg cggctgcggg acggcgactt tgtcatcgac
     2041 atctactaca agcgggtgct gaccagcgaa ttgcttgaga agcccgacgt ggcacgtgct
     2101 acgatacagg cttatgaggc ggggaaaatt tgcgtcatca actcgttccg cgccaagttc
     2161 ttgcacaaga agatgagcct ggggctgatg cacgacgacg ccaacgccca cctgttcagc
     2221 gagcacgagc ttgacgtgat ccggcggcac atcccctgga cgcgcaaggt ggcggagggg
     2281 catactacgc tgaacgggca gagcatcgac ttgctgccgt tcatcgcgga caaccgcgag
     2341 agcctggtgc tgaagccgaa cgacgagtac ggcggcaagg gggtcgtgat tgggtggacg
     2401 gcctcggacg aggagtggca gcgggcgata aaggaggcgc tggaaagctc cttcgtggtc
     2461 caatgggccg tgcagctcga ccaggagccg tacccttact acgaccagca gcagggggtc
     2521 agcttccgcg acctcaccgc cgacctcgac ccgttcgtgt tcgggccgga catggcgggg
     2581 atactgaccc ggctgtcggc ggcggccctg ttgaacgtca ccgcgggcac agggtcggtg
     2641 gtgccgacca tgatagtgga gaaggacccg atcccggcga gagcaaggtg aggcgcgggc
     2701 gtggggcagc ctccaggtga taccggcgag tcagaggggg agtagcgatg cagaagttct
     2761 tcacaatcgg ggttgaagaa gagttccaga tagtagaccc ttcgacgcgc gacctcaagc
     2821 cgtttgtcga gcagatgctg tcgcacgggg acgaggactt catcgagcac gtcaagcgcg
     2881 agatgataca gtcgatggtg gagacggtaa ccccggtgtg ccagaacgtg gaggaggcgc
     2941 gcagggaggt ctaccggctg cgggccgggg tggcggagct tgcggcgcgc aacgggctgg
     3001 cgatagtggc ctccggggca cacccgttct cccactggca gagccagatt atcaccgagc
     3061 acgaacgcta caaggtgctg gagcaggacc tgcaagacgt ggtgcgctcc atcctcattt
     3121 tcgggttgca cgtgcacgtg ggcatacccg accgcgacct gctcattgac ataatgaacg
     3181 aagcgcgcta cttcttgccg cacatgctgg ccctctcgtc caactcgcct ttctggcagg
     3241 gcaggcatac ggggttgaag tcgtaccgca cgattatctg gcagcagttc ccacgcacgg
     3301 gcataccttc cacgctgggg tcgtggtcgg acttcgagaa cttcgtgaac ctgctcatca
     3361 agaccaacag tatcgacaac ggcaagaaga tctggtggga cctgcgcccg cacccgtact
     3421 tcagcacgct ggagttcagg gtgtgcgaca tgcccaccaa cccggccgag acgatcatga
     3481 tcgtggcgtt cttccaggcg attgtcgcca agctgtacaa actgcgccag aagaacctcg
     3541 gcttccgcat ctacgaccgc gccctgagta tggagaacaa gtggcgggcg gcgcgatacg
     3601 gcctcgacgg catgctgatt gacttcggca aggagcagga agtgccgatg cgccagctcg
     3661 cgctcgagct gctggacttc gtggacgacg tggtggacga cctgggcagc cgggaagcgg
     3721 tgaactacat acaccaggtg ttgcagcagg gcaccggggc cgaccggcag atggaagtct
     3781 tccgccgcac gggcgacctc aacgacgtgg tagattacct catctacgag accaccaatg
     3841 gcctcgactg gcagggctac gggcgataag agacggaacc gccaagacgc caagagcacg
     3901 acaagagtac gaaaacgcaa agaacgcgca aaggttaagg agtatttaca gaataaaaaa
     3961 aggaccagag tcatgatggc gtgccctggt ccttttgctt agaacaccta cttaatcttg
     4021 gcgtcgttct tggcgcggcc ttggcgatct tggcggtccc gtctcttctt gttgctttgg
     4081 tatggcttgc cctaatttgg ggaaa
//
LOCUS       DASRWZ010000166         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_150812, whole genome shotgun sequence.
ACCESSION   DASRWZ010000166 DASRWZ010000000
VERSION     DASRWZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150812"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFH60_02490"
     CDS             <1..373
                     /locus_tag="VFH60_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6312677.1"
                     /translation="LAEGNWSPESGELAFTRLRAQFPEMDAVFASNDQMAQGVLHAAW
                     KENVRVPADLAVVGFDDIPEAAYFIPPLTTIRQETNELGRAAVRVLDQLITARRETGP
                     GEAYQPVSLEPRLIVRGSSVL"
     gene            424..2196
                     /gene="araB"
                     /locus_tag="VFH60_02495"
     CDS             424..2196
                     /gene="araB"
                     /locus_tag="VFH60_02495"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HET6312678.1"
                     /translation="MKCAIGVDFGTESGRAVLVDITDGRELAGSVHRYANGVIDEQLP
                     GSDISLEPDWALQDPNDYIEVFKHAVPAVLRESGVDPRDVIGIGIDFTACTMLPTRSD
                     GTPLCFLPEYRRQPHAWVKLWKHHAAQPEADLINEVARQRGEAWLARYGGKISSEWFF
                     SKALQILTEAPHIYRAADRLIEAADWVVWQLTGQETRNACTAGYKAIWQKETGFPSRE
                     YFAALHPGMADIVDAKLSREIAPLGARAGTLSEEAAGWTGLLPGTAVAVANVDAHVTL
                     PATGSIEAGTMVMIMGTSTCHVMVGEKQAESPGVADVPDVPGMCGVVEDGIVPGMLGY
                     EAGQSGVGDIFAWFVEHGVPPEYHQEARQRGMDLHTLLEEKAAKLKPGESGLVALDWW
                     NGNRSTLVDAELSGLIVGMTLATEAPEIYRALIEATAYGTREIIETLDKSGVSVRRLV
                     AAGGLPERNKLLMQIYADVTGREIAVIRSPQAPAVGSAMHAAVAAGVEAGGSPDIATA
                     ARHMGGLREDKYVPVPEHARVYERLYSEYKTLYDYFGRGQNEVMKRLRALRREALTPL
                     ERAEEADLQSNALPDATEQQAFSG"
     gene            2235..>2811
                     /locus_tag="VFH60_02500"
     CDS             2235..>2811
                     /locus_tag="VFH60_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET6312679.1"
                     /translation="MLEHLKEELYTLHLELVKYNLVVWTGGNVSARDPESGLVVIKPS
                     GVRYEHMRPEHMVVLDPEGRKVEGELKPSSDTASHLYIYRHRPDVGGVVHTHSPYASA
                     FAANGRPIPVVLTAIADEFGGPIPCGGFSLIGDESIGKVVLESIGRSSAVLLKNHGVF
                     TIGKNAEAAVKAAVMTEDNARSVWLALQLGTP"
BASE COUNT          564 a          840 c          945 g          462 t
ORIGIN      
        1 acttgcggag ggcaactgga gtcccgaaag tggagagctt gcattcacga ggctacgagc
       61 gcagttcccc gaaatggatg ctgtctttgc ttcgaacgac cagatggccc agggggtgct
      121 tcacgctgcc tggaaagaga acgtgcgcgt acctgcggac ctggccgtcg tggggtttga
      181 cgatattccc gaggccgcgt acttcattcc gccgctgact acaattcgac aggaaaccaa
      241 cgaactgggg cgggcggcgg tcagagtcct ggaccagcta atcacagccc ggcgtgagac
      301 cggcccagga gaggcatacc agccggttag cctggaaccc cggctgatcg tgcgcggtag
      361 ctcggtctta taagtcccac aagggaccac atgcatgatt gtcgtacgtc aagggggccg
      421 tacatgaaat gcgccatagg ggtagatttc gggaccgagt cgggtagggc ggtgctagtt
      481 gacataactg acggacgcga gttagccggt agcgtccacc ggtatgccaa cggcgtaatc
      541 gatgagcaac tacccggcag cgatattagc ctggagccgg actgggcgct gcaagacccc
      601 aatgactaca tagaagtctt caagcacgcg gtacctgccg tgctccgcga gagcggtgta
      661 gaccctcgtg acgtgatcgg cataggcata gatttcaccg cctgcactat gctccccacc
      721 agatcggacg gcacccccct ctgcttcctc cccgaatacc ggcggcaacc tcacgcctgg
      781 gtcaagctgt ggaagcacca cgccgcccaa ccggaggccg acctcatcaa cgaggtagcc
      841 cggcagcgag gcgaggcgtg gctcgcgcgg tatggcggca aaatctcctc ggaatggttc
      901 ttctcaaagg ccctgcagat cctcaccgaa gccccccaca tctaccgggc ggcagacagg
      961 ctgatcgagg ccgccgactg ggtagtgtgg cagcttaccg gccaggagac gcgtaacgcc
     1021 tgcaccgccg ggtacaaggc tatctggcaa aaggagaccg gcttcccctc tcgcgagtac
     1081 ttcgccgccc tccaccctgg tatggccgac attgtggatg cgaagctgag ccgcgagata
     1141 gcgccgcttg gagccagggc gggtacgctg tccgaagagg cggcagggtg gaccgggctg
     1201 ctacccggca ccgccgtggc cgtggcgaac gtggacgcgc acgtcacttt gcccgccacc
     1261 ggcagcatag aagcaggcac gatggtgatg ataatgggca ccagcacctg ccacgtcatg
     1321 gtgggtgaga agcaggcaga atcccccggt gtggcggatg ttcccgacgt gcccggcatg
     1381 tgcggggtgg tggaggacgg catcgtgccg gggatgctcg gctacgaggc ggggcagagt
     1441 ggggtagggg acattttcgc ctggttcgtc gagcacgggg tgcccccgga gtaccaccag
     1501 gaagccaggc aacgcggtat ggacctgcac acgctgctgg aggagaaagc ggccaaactc
     1561 aaaccgggcg agagcggcct ggtcgcgctc gattggtgga acggcaaccg ctccacgctg
     1621 gtagacgcgg agttgagcgg cctcatcgtg ggcatgacgc tggcgaccga ggccccggag
     1681 atataccgcg ccctgattga ggccacggcc tatggcaccc gcgagattat agagacgctc
     1741 gataagagtg gggtcagcgt gcgccgcctg gtggccgcgg gggggctacc cgaacgcaac
     1801 aagctgctca tgcagattta cgccgacgtg accggccgcg agattgccgt catacgcagc
     1861 ccccaggccc cggcggtggg ttctgccatg cacgcggccg tggcggcggg tgtcgaggcg
     1921 gggggctccc ccgacatagc caccgctgcc cggcacatgg gaggcttgcg agaagataag
     1981 tacgtcccag ttcctgagca cgcgcgcgtg tacgagcgtc tctactcgga gtacaagacg
     2041 ctgtacgact actttggaag gggccagaac gaggtgatga agcggcttcg cgccctgcga
     2101 cgagaagcgt tgactcccct ggagagggcc gaagaagccg accttcaaag caatgccctg
     2161 ccggacgcta cggaacagca ggcgttcagc gggtaagcct cggctgagta gagaaaggac
     2221 ggggagcacc tgtcatgttg gagcatctta aagaagagct atacacgctg cacctggagc
     2281 ttgtgaagta caacctggtg gtgtggaccg ggggcaacgt cagcgcgcgc gaccctgaga
     2341 gcgggctggt ggtcatcaag ccgtcgggcg tgcgctacga gcatatgcgg cccgagcaca
     2401 tggtggtgct cgacccggaa gggcggaagg tggaagggga gcttaagccc tcgtcggata
     2461 cggccagcca cctgtacatc taccgccaca gacctgacgt tggcggggtg gtgcacactc
     2521 actctccgta tgccagcgcc ttcgccgcca atggccgccc tatccccgtg gtgctcaccg
     2581 ccatcgcgga cgagttcggc ggccccattc cttgcggggg tttctcactt attggcgacg
     2641 agtcgatagg caaggtagtg ctggagagca taggccgctc ttccgccgtg ctgctcaaga
     2701 accacggggt atttactata ggcaagaacg ccgaggcggc ggtcaaggcg gccgtaatga
     2761 ctgaggacaa cgcacgcagt gtgtggctgg cccttcaact gggcaccccc g
//
LOCUS       DASRWZ010000167         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_287907, whole genome shotgun sequence.
ACCESSION   DASRWZ010000167 DASRWZ010000000
VERSION     DASRWZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287907"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VFH60_02505"
     CDS             <1..1031
                     /locus_tag="VFH60_02505"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET6312680.1"
                     /translation="DDLRLSRDQLDVIIHCVADGIVVQDPAARVIFANAAGVQSLGII
                     SLKALRDMPFDQVLHQFEFVTETGEICSRERLPAMRALHAEQVPDEVFCFRNRMTGHE
                     RWYILNATPVFDRHGQVQFAVSIFKEISERKRVERALLELSVRDELTGLYNRRELNRL
                     LTDEVNRYRRYRRPAALVLVDIDHFKLVNDNFGHQAGDMVLRRVAELMQSQLRASDRV
                     ARFGGEEFAIVLPETTASEAYDVAEDLRQSLAALRCRYVSGDGTALDLAVTVSLGVAG
                     LLDDMGGVDEWVESADAALYFSKRTGRNKTTCAIAPEHGGAHTVSSQWATLVYDNALP
                     VAANGMHD"
     gene            1367..1930
                     /locus_tag="VFH60_02510"
     CDS             1367..1930
                     /locus_tag="VFH60_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010548556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HET6312681.1"
                     /translation="MTDDSQLETAEDLLTRAHELLLRTGLTVSVAESCTGGLLGAALT
                     ERSGSSAYFLGGVLAYADEVKTGLLGVDGEVIAAHGAVSRPVALGMAGGVRRVIGSDL
                     AISITGIAGPTGGTDDKPVGTTFIGLSGLGTERVERYVWQGGRAENRANSVRAALQML
                     VQLIEGNMSAVSPVPLPRPHGEATDGA"
     gene            1951..>2138
                     /locus_tag="VFH60_02515"
     CDS             1951..>2138
                     /locus_tag="VFH60_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET6312682.1"
                     /translation="MYLKTTLPNGLRIVTFQMPHTRSAAVQFFVRVGARNESMEKSGI
                     SHFLEHMVFKGTEHRPKPV"
BASE COUNT          409 a          654 c          669 g          406 t
ORIGIN      
        1 cggacgactt gcgcctctcg cgcgaccaac tggacgtcat aattcactgc gtggcggatg
       61 gcatcgtagt gcaggacccc gcggcgaggg taatattcgc caatgccgcc ggagtgcaaa
      121 gcctgggcat catttcgctc aaggccctgc gcgacatgcc cttcgaccag gtgctccacc
      181 agttcgagtt cgtgactgaa accggcgaaa tttgctcgcg cgagcggctt ccggcaatgc
      241 gcgccctcca cgccgagcag gtgcccgacg aggtcttctg cttccgcaac cggatgacag
      301 gacacgagcg ctggtatatc ctcaatgcca cccccgtgtt cgaccgccac gggcaggtgc
      361 agttcgcagt cagcatcttc aaggagatca gcgagcgcaa gcgcgtggag cgcgccctgt
      421 tggagctttc ggtgcgcgac gaattgaccg ggctgtacaa ccggcgcgag ttgaaccggc
      481 tgctgacgga tgaagtaaat cgctaccgcc gctacaggcg tcctgctgcc ctggtgctgg
      541 tggacatcga ccacttcaag ctggtcaacg acaactttgg acaccaggcc ggcgacatgg
      601 tgctcaggcg tgtggccgag ttgatgcagt ctcagcttcg cgccagtgac agggtagctc
      661 gtttcggggg tgaagagttc gcgattgttc tgccggagac gactgccagt gaggcgtatg
      721 acgtggccga ggacctgcgc cagtcgctcg ccgcgctaag gtgtaggtac gtatcgggcg
      781 acggtaccgc tcttgacctg gcggtgacgg tgagcctggg ggtggccgga ctgctggacg
      841 acatgggcgg cgtagatgaa tgggtcgagt ctgccgatgc ggcgctctac ttctccaagc
      901 gcacagggcg caacaagacg acttgcgcga tagcccccga gcatgggggc gcgcacaccg
      961 tgtcgtcgca atgggctacc ctcgtctacg ataacgccct gcccgtcgcc gcaaatggca
     1021 tgcatgactg actgcaatat atccaggtgc taaaaaccgc gaacaccgat ggagtcgggc
     1081 actccgggct gcgctgaccc cgcaacgggt cccggacgcc tttcttccct ggttatccca
     1141 gtcccgcaag cttccaaaga tgttccaccg ggcatgccgt ggcccttccg gcatgttgcc
     1201 gcaaagtcca agagcgtcaa tctcgacctt ctatcatgct ctaccttctt gagattgctc
     1261 gctgcgctca gcatgcggct cggctgccac gacgcatgac tctcttggta cgctggagag
     1321 cgtgtggtat acttttctcc aatcatccat gcgaccgctc ttgaaaatga ccgacgactc
     1381 ccaactggaa acagccgaag atctactcac ccgtgcccac gaattgttgc tgcgcaccgg
     1441 cctgaccgtg tctgtcgcgg aatcgtgtac gggcgggctg cttggcgcgg cgctgacgga
     1501 gcgttccggc tcgtctgctt acttcctggg cggggtgctc gcctacgcgg atgaggtcaa
     1561 gaccggcctg ctcggcgtgg atggagaggt catagccgcc cacggagctg tcagcaggcc
     1621 agtagccctc gggatggcgg ggggcgttcg ccgcgtaatc ggctctgacc tcgccatctc
     1681 gattacgggc atagccgggc ccacgggcgg caccgatgac aagccggtgg gcacgacctt
     1741 catcggcctg tccggtttgg gaacggagcg cgtggaaagg tacgtctggc aaggcggcag
     1801 ggctgagaat cgagccaact cggtacgcgc cgcgttgcaa atgctcgtgc aactgataga
     1861 aggtaacatg tccgctgtgt ctcccgtgcc gcttccccgc ccgcatggag aagcaacaga
     1921 cggcgcgtaa tactagatta gagagaaaca ttgtacctga agaccacgct ccctaacggg
     1981 ctgcgaatag taacattcca gatgccgcat acgaggtcgg cggcagtcca attcttcgtg
     2041 cgcgtcggcg cgcgcaacga gagcatggaa aagagcggca tcagccactt cctcgagcat
     2101 atggtgttca agggcaccga gcaccgcccg aagcctgt
//
LOCUS       DASRWZ010000168         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_972868, whole genome shotgun sequence.
ACCESSION   DASRWZ010000168 DASRWZ010000000
VERSION     DASRWZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972868"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2621)
                     /locus_tag="VFH60_02520"
     CDS             complement(<1..2621)
                     /locus_tag="VFH60_02520"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET6312683.1"
                     /translation="MAFQPEVNQQLSIDGITYMVAEHPVARGIPYGQEGRQAVVYQLI
                     AGADKRAIKAFKSRYQMPSMVSLAKRLAPYAALPGLQVCRRSVLSPQQHEALLNAYPD
                     LIYAVLMPWIEGPTWMEVLLEKIELTRDQSLTLARALAGILAGMEQEGLAHCDLSGPN
                     LLLPMMASGPTSYQTPVALVDVEQMYGPDLRRPEVVPGGSAGYAHKTAPEGLWSSDAD
                     RFAGAVLMAEILSWCDPRVREACWGESYFDPQEMQTDGERYRLLVTALTEAWGGNVAR
                     MFERAWTSQSLADCPSFGQWLVSLPKSVPGAAATQPEPEAIAGTSEVSVRTLMELASR
                     FEAQGNLTSALATYQQARSLPDVGSALASELTLIIDELENKLSPQPAAPAAPAPATQA
                     APTPAPQPQAPPRRGRVDSLFDQGLGAYNRGEWREARELLTEVVRSEPNYTFAGQSAA
                     TLLAESERRIAAASQPAAPVAPVPQATQGTQTVPITPDDAPAAQTARAEVKSSPSTID
                     RLTRIVVPATLGLLLLIFGGIVFLQLQGTPKPPASGNDGGPGGAQNTPSGPDALSSPV
                     PEDTDAFLGLIRFHDGSNALDEVSFNIEKIEDFPAPPAGKQYQVWLLGAGGERRRSLG
                     ILKLEGEGSGNLEMTDEEGANLLKLYDAFEITLEPDPDSSPELPSSDVAFSGALPSEA
                     YMHMKHVLVETEETPEQVGSTLGLLREADLVLTIAKEMVKAQESGDLERMRLDAEGLL
                     NLIEGEGGANFGDVNQDGRVTTFGDGFGLLLNGDRLGYIQLVIAHTTNTVRAPDATNN
                     IKIHADHTIVSAQNLGDWVGELRTLTLNIAKSTSVEEAAGDVRKASALADRILNGHDL
                     NGNEQVEPIKGEGGAKTA"
     gene            complement(2720..3034)
                     /locus_tag="VFH60_02525"
     CDS             complement(2720..3034)
                     /locus_tag="VFH60_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312684.1"
                     /translation="MPPVTEGEPPPPGTEPPPVPGDVPPVGTEPPPPVEGAPPPPPAD
                     VPQEAIPGEPPPTDIPPEGAPGEQPPADVPTEGTPGEQPPAEVPPESVPEEQPPPEDR
                     QG"
     gene            complement(3022..>3282)
                     /locus_tag="VFH60_02530"
     CDS             complement(3022..>3282)
                     /locus_tag="VFH60_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312685.1"
                     /translation="LRLSRHQLSRHQLSHRQWNHLRLSHRQWNHLRWNRRLLSLHQWN
                     RHLLSLRLGLRRNHNLSRNRNLRLSRRPNLRLGRLGSLPCPR"
BASE COUNT          501 a         1032 c         1075 g          674 t
ORIGIN      
        1 gcggtcttcg cgccaccctc gccctttatc ggctcgacct gttcgttgcc gttgaggtca
       61 tggccgttga ggattctgtc cgccagggca gaggcctttc gcacgtcccc tgccgcctcc
      121 tccacagatg tgctcttggc gatattcaag gtaagcgtgc gcaattcccc tacccaatcg
      181 cccaggtttt gtgcggagac gattgtgtgg tccgcgtgga tttttatatt attggtggcg
      241 tctggagcgc gtacagtgtt agtcgtgtgc gcgatcacca attggatgta accaagccgg
      301 tctccgttca gcagcagtcc gaaaccatca ccgaaggtcg tcactctgcc gtcctggttc
      361 acgtccccga agttcgcccc tccctcaccc tctatgaggt tcaacaaacc ctcggcgtcg
      421 agcctcattc tctccaggtc acccgactct tgcgccttga ccatctcctt tgcgatagtt
      481 aaaaccaggt ccgcctcgcg caacagccca agcgtcgaac ctacctgctc cggcgtttcc
      541 tcagtctcta ccaatacgtg tttcatatgc atataagcct ctgatggcag cgcgcccgaa
      601 aaggctacat cgctgctggg tagctcaggg ctgctgtccg ggtcaggttc gagagtgatt
      661 tcaaaggcat cgtagagctt gaggagattg gccccttctt cgtcggtcat ctccaggttg
      721 ccactccctt ccccctccaa tttgagtatg ccgagtgagc ggcgtctctc gccacccgcg
      781 cccaggagcc atacctggta ttgcttaccg gcaggcggcg cggggaagtc ctcgatcttc
      841 tcgatattga agctgacctc atcaagagca ttagacccgt cgtggaagcg tatcaggccg
      901 agaaatgcat cggtatcttc cggcacgggg gatgacaatg cgtccggccc tgacggcgtg
      961 ttctgcgcgc cgccagggcc gccgtcgttg cccgatgcag gcggtttggg cgtgccctgc
     1021 aattgcagga agacgatacc gccgaaaatc aacagcaaca gccccagcgt cgccgggacc
     1081 actatcctgg tcaacctgtc tatcgtgctg ggggaggact tgacctctgc ccgtgcggtc
     1141 tgcgccgccg gtgcgtcgtc gggcgtaatg ggcacggttt gtgtgccctg agtggcctgc
     1201 ggtacgggcg ctacaggcgc ggcaggttgg gatgcggctg cgatacgccg ttcgctttca
     1261 gcgagcaggg ttgccgcgct ctggcccgcg aatgtgtagt tcggctcgga gcgcaccact
     1321 tcggtgagca attcccttgc ctcccgccac tcgccccggt tgtaggcacc gagaccctgg
     1381 tcgaagaggc tgtccacacg gcctctgcgg ggaggggcct ggggttgggg cgcgggggtt
     1441 ggagcggcct gcgttgcggg cgctggcgca gcaggggcag caggctgggg cgacagctta
     1501 ttctccaact catctatgat cagggtaagc tcgctggcga gcgcgctccc cacgtccggg
     1561 agagaccgcg cctgctggta ggtagccagc gcgctggtca gattgccttg cgcctcgaag
     1621 cggctcgcca actccatgag cgtgcggacg ctgacttccg acgtgcccgc aatcgcttca
     1681 ggttcgggct gtgttgcggc ggctccgggc acgctcttgg gcaggctcac cagccactgg
     1741 ccgaaagaag ggcagtcggc tagcgactgg ctggtccacg ccctctcgaa catgcgcgcc
     1801 acgttgccgc cccatgcctc ggtcagggcg gtgacgagca ggcggtagcg ctcgccgtcg
     1861 gtttgcatct cctgggggtc gaagtagctc tcgccccagc acgcttctcg cactcgcggg
     1921 tcgcaccagc tgagtatctc cgccatgagc accgccccgg cgaagcggtc cgcgtcggac
     1981 gaccacaaac cctccggggc ggtcttgtgc gcgtagcctg ccgagcctcc gggcactact
     2041 tcgggccgcc ggaggtcggg gccgtacatc tgctcaacgt ctacgagggc gacgggcgtc
     2101 tggtagctag tggggccgga cgccatcata ggtaatagca ggttggggcc ggacaggtcg
     2161 cagtgggcaa gcccttcttg ctccattccc gccaatatgc ccgccagggc gcgagcgagc
     2221 gtgagggact ggtctcgggt aagctcgatc ttttccagca gcacttccat ccaggtgggg
     2281 ccttctatcc agggcatgag caccgcgtag ataaggtcgg gataggcgtt caagagggct
     2341 tcatgctgct gtggggaaag gacgcttcga cggcatacct gcaagccggg cagggctgcg
     2401 tagggcgcga ggcgcttggc tagactcacc atactgggca tctggtagcg cgacttgaac
     2461 gctttgatag ccctcttgtc ggcgcctgct atcagttggt atacaacggc ctgcctgcct
     2521 tcctgcccat acgggatgcc gcgggcgacg gggtgctcgg caaccatgta ggttataccg
     2581 tcaatgctca gttgctggtt gacttccggc tgaaacgcca tctatatctc cttttcatcg
     2641 cgctcaacga gaagcgtagc gcgctgctgg ccctccgcca gtgatgtggc atgtccacac
     2701 taccggcaac gctatgctgt tatccttgcc tatcctcagg cgggggttgt tcctccggca
     2761 ctgactccgg gggcacctca gccggtggct gctcccctgg cgttccttcc gtaggcacgt
     2821 cggcaggtgg ctgctcccct ggcgctccct ccgggggtat gtcggttggc ggcggctcac
     2881 cgggtattgc ttcctgtggc acgtcggctg gcggcggtgg gggtgcgccc tctaccggcg
     2941 gtggcggttc tgtgccaacg ggcggcacgt cgccagggac aggaggcggt tccgtgccgg
     3001 gcgggggcgg ctcaccctcg gtcaccgggg gcacgggagg ctccccaacc ggcccaggcg
     3061 gaggttcggg cgccggctca ggcgcaggtt ccggttgcgg ctcaggttgt ggttccggcg
     3121 caggcccagg cggaggctca gcaggtggcg gttccactgg tggaggctca gcaggcggcg
     3181 gttccaccgg aggtggttcc actggcggtg gctcaaccgg aggtggttcc actggcggtg
     3241 gctcaactgg tggcggctca actggtggcg gctcagccgg ag
//
LOCUS       DASRWZ010000169         1612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_288039, whole genome shotgun sequence.
ACCESSION   DASRWZ010000169 DASRWZ010000000
VERSION     DASRWZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288039"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VFH60_02535"
     CDS             complement(<1..1037)
                     /locus_tag="VFH60_02535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6312686.1"
                     /translation="MTVSYWQDASHEQIIECDVCVVGAGIVGLYLARLIAGHGQRVCV
                     LEARHIASGASGRNAGMVLTGTAYYYHDAIRVYGPELARDLWLLSLHNRDIARGLSEE
                     FGTHWEHSGSLLLALDDTEAEDIERAADAMLDDGIEAKFSHKDPLKRGFTAVVSQPGD
                     AGIHPVKFAQALAASSGATIYEQSEVFDIAPMPGGGVELKSRRATVRCRAAALCTNAF
                     APLLHPYFEGKVMPTRGQILRTEPLGTRLFSQMCYADYGYEYFRQLPDGSLLMGGWRH
                     HFREAEVGYEDRTTRDVQGGLEGFIARYFPEAKGARVTHRWSGTMGFSADGIPLVGRL
                     PDIPDVYFAVG"
     gene            complement(1038..>1612)
                     /locus_tag="VFH60_02540"
     CDS             complement(1038..>1612)
                     /locus_tag="VFH60_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312687.1"
                     /translation="QGKPIPGQEAQPPPAPAPEPDPFANVPPPFRQEPPAIASGAPPP
                     VAEQAPTWTPGTPRPQPGTPEYEALVRAALAQRGVTPAPPSPQPTYTPPPAPTQQPAA
                     WTPGMPRPKPGTPEYEAMAKAALEAARQGNSAPTGPQPQNAPPPGETSTSSGGGFWAA
                     DKPRPQPGTPEYEALVREALNQQRKAREGG"
BASE COUNT          248 a          505 c          569 g          290 t
ORIGIN      
        1 aagccaacgg caaagtaaac atcgggtata tcgggcaggc gtcctaccag gggtatgccg
       61 tccgcgctga agcccatcgt gcccgaccag cgatgggtaa cgcgcgcgcc cttcgcttcg
      121 gggaagtagc gcgctataaa accctcaagg ccgccctgca cgtcgcgggt ggtgcggtcc
      181 tcatacccaa cctcggcctc gcggaaatgg tgccgccagc cgcccatcag caggctgccg
      241 tcgggcaact gccggaaata ctcgtagccg taatccgcgt agcacatctg cgagaagagc
      301 cgggtgccca ggggttcggt gcgcaatatc tggccgcgag tgggcatcac tttgccctcg
      361 aagtaggggt gcagcagggg cgcgaaggcg ttggtgcaga gggcggcggc ccggcagcgc
      421 acggtagcgc ggcgcgattt caactccacg cccccacccg gcataggggc tatatcgaat
      481 acctccgatt gctcgtagat ggtcgcgccg gaggaggccg ccagcgcctg ggcgaacttg
      541 accgggtgga tgcccgcgtc ccccggctgc gatactacag ccgtgaagcc gcgcttcagg
      601 gggtctttgt ggctgaattt agcctctatg ccgtcgtcca gcatagcgtc cgccgcccgc
      661 tcgatgtcct cggcttccgt gtcatcgaga gcaagtagaa gcgaaccgct gtgctcccag
      721 tgggtgccga actcttcgga aaggccgcgc gctatgtcgc ggttgtgcaa gctgagcagc
      781 cagaggtcgc gggcaagttc cggcccgtag actcgtatag cgtcatggta gtagtaggcc
      841 gtgcctgtga gcaccatgcc cgcgttgcgc cccgacgcgc ccgacgcgat atgccgcgct
      901 tcgagcacgc acaccctctg cccgtgacca gcgatgaggc gagccaggta gaggccgacg
      961 atccccgcgc ccaccacgca cacgtcgcac tcgatgatct gctcgtggct ggcgtcctgc
     1021 cagtaggaga cggtcaacta gcccccttca cgcgctttgc gctgctggtt gagggcttcc
     1081 cttaccagag cctcgtattc cggcgtgccg ggttgagggc gcggcttgtc ggcagcccag
     1141 aacccgccgc ctgaactggt agaagtctcg ccgggtggtg gtgcgttctg tggttgcggg
     1201 cctgtggggg cgctgtttcc ctgccgtgca gcttccaggg ctgctttcgc catagcctcg
     1261 tattcgggcg tgcccggttt gggccgcggc ataccgggcg tccaggcagc cggttgctgg
     1321 gtgggagcag gagggggtgt atacgtaggc tggggcgagg gtggcgctgg cgtaacccct
     1381 ctctgcgcaa gggcggccct caccaaagcc tcatattcgg gcgtgccggg ctgaggccgg
     1441 ggtgtaccgg gcgtccaggt gggggcctgc tcagccacag gcggaggcgc gccggaggct
     1501 atggcgggcg gctcttgcct gaacggtggg ggtacattgg cgaaaggatc aggctccggt
     1561 gcgggcgcgg gcggtggttg cgcctcctgg ccgggtatgg gcttgccttg ct
//
LOCUS       DASRWZ010000170         6127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_562384, whole genome shotgun sequence.
ACCESSION   DASRWZ010000170 DASRWZ010000000
VERSION     DASRWZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562384"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..696)
                     /locus_tag="VFH60_02545"
     CDS             complement(121..696)
                     /locus_tag="VFH60_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6312688.1"
                     /translation="MSPLGKRILVLGMFWVFLVMIVTQVYDNVTGRGMPVTRAAAPTP
                     TAPVEADATIQQLADLQACVANNPNNLDCMLDLANLYYDLGQYAQSQTAFEAAVKLDP
                     HNVGALVKLAGSYIRQNNFNQAVTTLEEAIRLQPDSPELHLLLGLSVSKLEPPQMERA
                     VAEWRQVIKLDPDSALADQARLYIQEAGQQP"
     gene            complement(693..1259)
                     /locus_tag="VFH60_02550"
     CDS             complement(693..1259)
                     /locus_tag="VFH60_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312689.1"
                     /translation="MQELGKRIGMLRLLLADIQSKQTQLEEMEGQYRAQLARIVDFVV
                     YRDGDVSNALSLMSEVQSKLNEVTQTEDHLAQIAEKANLELEVLVLTKRVAEARSQLA
                     ELEARQKELSSRLAYMSEGEPAMGEDQDLQPRQRDHIRSINDEVMEVSSEIERLNQLI
                     TEASERAALTIHVSARKVPDAARKREAE"
     gene            1636..2595
                     /locus_tag="VFH60_02555"
     CDS             1636..2595
                     /locus_tag="VFH60_02555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6312690.1"
                     /translation="MALDNFVSQADGLGIVVTPEEQRVIFSRPGFHPERRVLVVEGPK
                     FDGVADGTLLGYATLPSVEDEETGERMYTANLFVHPAAREHGLQRVLMGRLVEIARQE
                     EAALEGKARGQAEIRIDASEKQRYIRALSQEIGLNLARQFWMMECPLVKLDEPQAIEG
                     VTIRSYRVPADHPAAMEAYNQSFRDHYGFHPTTPDRWEYRFADPTMKADLSWIAEIDG
                     EPGKIAGFCLCNVYEEENKTLDRCEGWIGLLGTVRDWRGKGLGRSLLLHGLHSLRASG
                     LDTGVLGVDSDSPTGANRLYESVGFRTREIWLQFRAPLAEVRL"
     gene            2625..3269
                     /locus_tag="VFH60_02560"
     CDS             2625..3269
                     /locus_tag="VFH60_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6312691.1"
                     /translation="MPPKDLTRAETSYDLGRVYMERGQYELAAEQFRQALVASPHNPA
                     ALYQLGKALEFLGRIDETLQAYEQAVSAPANPNVGHSTGSDYRAEAKQGIDRLRKLKE
                     YLAKVSYYEAKVNRAIWHVREVMRESTPPIVDQIYFGAMDIDPKNLFVAFAYADSEAL
                     ERAKQQGHFDLIRREFLAALQEAGYPTHALPSDKIDFVSKQEVDEQGGPWIYFR"
     gene            complement(3435..4229)
                     /locus_tag="VFH60_02565"
     CDS             complement(3435..4229)
                     /locus_tag="VFH60_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312692.1"
                     /translation="MCLVLAFIIAVSYVQVALQAQANVTGLLAIPTSRSVKLSALPAE
                     GTATAVAAKTQVAKEPKGRIRTRDHGSWSYEASREESGLVVATVRYDSKTAEGVRAYA
                     AANKQLAEELATSGNEAIVKVTFKKPFPVDAYRAWAASSGVTQFDYVYTRVKNSAGRD
                     GMFSVVVHSQDPLPQSGLDSFMLSSKQLDNGPYTVLGVIYFEGRIAADRLVELSRDPN
                     VFIADVTHNIVVAELVQDGIYLADRTHVSPVAVFGVLESLGLHNFK"
     gene            4679..>6127
                     /gene="hutU"
                     /locus_tag="VFH60_02570"
     CDS             4679..>6127
                     /gene="hutU"
                     /locus_tag="VFH60_02570"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET6312693.1"
                     /translation="METMTSLRAPRGTTLQCKGWLQEAALRMLMNNLDPDVAERPQDL
                     VVYGGRGKAARDWPSLLKILDCLRNLENDETLLVQSGKPVGVFKTHTDAPRVLIANSN
                     LVPHWATREVFSRLEAQGLTMYGQMTAGSWIYIGTQGILQGTYETFGAAARKHYGPEA
                     DLSGKLVLTAGLGGMGGAQPLAVTMNGGVAIVVEVDQTRIQRRLDTRYLDTATDNLED
                     ALQIAEAATRERRPLSIGLLGNAAAIYPEMVRRGSIPDMVTDQTSAHDPLNGYVPLGY
                     DLDSAAALRHSDPGEYVRMSLESMAVHVRAMLDLQRMGATVFDYGNNIRQQAFGQGVT
                     DAFDFPGFVPAYIRPLFCEGKGPFRWAALSGDAEDIYTIDDMVLREFPDNASVQRWIP
                     LAREKVAFQGLPARICWLGYGERAHFGQVVNEMVRTGQVSAPIVIGRDHLDTGSVASP
                     NRETEAMKDGSDAISDWPILNALINAVGGATWV"
BASE COUNT         1205 a         1969 c         1832 g         1121 t
ORIGIN      
        1 ctcagcccgt ggctttagcc acagggcgtc cccacagggc ggccgcaaga ggctattccc
       61 gccttctctc gcagtaaccg gtgccccacc ccagacatgc agagcacaca ccggcaaaat
      121 ctaaggctgc tgcccggcct cctggatgta caggcgggcc tggtccgcga gggccgagtc
      181 ggggtccagc ttgataacct gccgccattc cgcgacggcg cgctccatct gcggcggctc
      241 cagcttgctc acggacaggc cgagcaacag gtgcagttca gggctatcgg gttgcaagcg
      301 gatagcctct tccaacgtcg tcacagcctg gttgaagttg ttctggcgta tgtacgagcc
      361 tgccaacttg accagcgcgc ccacgttgtg cgggtcgagc ttgaccgcgg cctcgaacgc
      421 tgtttgcgac tgcgcgtact ggccgaggtc gtagtagagg ttcgccaggt cgagcatgca
      481 atcgaggttg ttcgggttgt tggctacgca ggcttgcagg tccgcgagtt gttgaatggt
      541 ggcgtcggcc tccacggggg ccgtgggcgt gggagccgcg gcgcgggtga cgggcatgcc
      601 ccgccccgtg acgttgtcgt acacctgggt gacgatcatc accaggaaca cccagaacat
      661 acctagcacc agtatcctct tgcccagcgg actcattcag cctctctttt cctggccgcg
      721 tccggtacct tgcgggcaga cacgtgtatg gtcagcgccg cccgttcgct cgcctcggtg
      781 atgagctggt tcagacgctc gatctcggag gacacttcca ttacctcgtc gtttatgctg
      841 cggatgtggt cgcgctgcct cggctgcagg tcctggtcct cacccatcgc cggctctccc
      901 tccgacatgt aagcaagccg cgaggatagc tccttctgcc gcgcctctaa ttctgccagt
      961 tgcgaccgtg cttccgctac gcgcttggtg agcacaagca cctcaagctc caggttggcc
     1021 ttctcagcaa tctgcgccag gtggtcctcg gtctgcgtca cctcgttcag cttactctgt
     1081 acctctgaca tgagggagag cgcgttagac acgtccccat ccctgtatac aacgaaatcg
     1141 acaatccgcg ccaactgcgc ccggtactgg ccttccattt cctccagttg cgtctgcttc
     1201 gactgtatgt ccgcaagcaa cagccgcaac atcccgatac gcttccctag ttcttgcatg
     1261 tgaaccccct cgctgtagtg ccgagtgccg agtgccgagt gccgagtagt ggtcagtggt
     1321 cagtggtcag tggtcagtgg taagtgagac gcaccaaaac cagcctaaac tttgctcgta
     1381 cttttggtcc ttcgcgtcgg gtctctttac tcggtactcg gtactcggta ctcggtactc
     1441 ggtactcggt actcggtact cggtactcgg tactcggtac tcggtactcg ttatgctata
     1501 atggcgcggc tgtccgcgca cccgaatgcg ggccgactta ctcttggagg ggaaacggtg
     1561 tctgttcctg gcaatgtgca tgcagcggta gattgggttg tgcggcccta tcgggaggac
     1621 gatttcgagg ctaccttggc gctggacaac ttcgtgtccc aggccgatgg cctcgggata
     1681 gtggtcaccc cggaggagca gcgggtcatc ttctcccggc ccggcttcca cccggagcgc
     1741 agggtgctgg ttgtcgaagg tccgaagttc gacggcgtgg ccgatgggac gctcctgggg
     1801 tacgccaccc tgccctcggt ggaagacgag gagaccggcg agcgcatgta caccgccaac
     1861 ctgttcgtgc accccgccgc cagggagcac ggcctccagc gcgtcttgat ggggcggctg
     1921 gtggagatcg cccgccagga ggaagcagcc ctggagggta aggcgcgcgg gcaggccgaa
     1981 atacgcatcg atgcctcgga gaagcagcgc tacatcagag cgctcagcca ggaaatcggg
     2041 ctgaaccttg ccaggcagtt ctggatgatg gagtgcccgc tcgtcaagct ggacgagcca
     2101 caggcgatag agggcgtcac gattcgcagc taccgcgtgc ccgccgacca ccccgccgca
     2161 atggaggcat acaaccagtc cttccgcgac cactatggct tccaccccac cacccccgac
     2221 cgctgggaat accgcttcgc ggaccccacc atgaaggccg acctctcctg gatagccgag
     2281 attgatgggg agccgggcaa gatagcgggc ttctgcctgt gcaacgtcta cgaagaggaa
     2341 aacaagacct tagaccgctg tgagggctgg ataggactgc tcgggacggt acgcgactgg
     2401 cgaggcaaag ggctaggtcg ctcgctgctg ctccacggcc tccattctct gcgcgcctcc
     2461 ggcctcgata cgggcgtgct gggcgtcgat tcggatagcc ccaccggcgc gaaccgcctg
     2521 tacgagtcgg tgggcttccg cacccgcgag atctggcttc agttccgcgc cccgctggca
     2581 gaggtgcgtc tctagcctag cgccgtgtgc cggaggagaa agtggtgccg cctaaagacc
     2641 ttacgcgggc tgaaacctcg tacgacctgg gccgcgtcta catggagcgg gggcagtacg
     2701 agcttgccgc cgagcagttc cgccaggccc tcgtcgcctc cccccacaac cccgccgcac
     2761 tctaccagct aggcaaagcc ctcgaattcc tcggccgcat cgatgagacg ctacaggcat
     2821 acgagcaagc cgtaagcgcc ccggccaacc ccaatgtggg gcactcgacc ggctcggact
     2881 accgggcaga ggccaaacaa ggcatagacc gcctccgcaa gctcaaagag tacctggcga
     2941 aagtgagcta ctacgaggcg aaggtcaacc gggccatctg gcacgtgcgc gaagtgatgc
     3001 gcgagtcgac gcccccaatc gtggaccaga tttacttcgg cgcgatggac atagacccca
     3061 aaaacctctt cgtcgccttc gcctacgccg actccgaggc gcttgagcgg gcaaagcagc
     3121 aaggccactt cgacctcata cgccgagagt tcctggccgc gctacaagaa gccggttacc
     3181 cgacccacgc cctcccctca gacaagatcg acttcgtctc caagcaggaa gttgacgaac
     3241 aaggtggacc gtggatttac ttcaggtaag cagcaccaga cgtatatcct cccctcctgg
     3301 agagtctgct gtcgtttcgc cgccaggatg ctggatgaca caggtgcccg tccgcgaaag
     3361 cgccggtcct aagctggcgg tacctaccgg catacggatg tagctgaagc gtgaaggtag
     3421 gctgttagtt gaggctattt gaagttgtgc aggcccaaac tctccaggac cccaaacaca
     3481 gccacgggcg atacgtgggt gcggtccgcg aggtagatgc cgtcttgtac aagctcggcg
     3541 acaacaatgt tatgggtaac atcggcaatg aagacattag ggtctcttga aagctcaacc
     3601 agtcgatcgg cggcaatccg gccctcgaag taaatgacac ctaatacagt gtatggtcca
     3661 ttgtccaact gtttcgaaga aagcataaag ctgtctagcc ctgactgggg aagggggtcc
     3721 tgggagtgta cgaccaccga gaacatgcca tctcgcccgg cgctattctt gacgcgagtg
     3781 taaacgtagt caaactgagt gacaccagat gacgcagccc aggcacgata cgcatctaca
     3841 ggaaatggtt tcttgaaagt aactttcaca attgcttcgt ttccggaggt ggcgagttcc
     3901 tcggcaagtt gcttattggc agcggcgtaa gcgcgcacac cttccgccgt tttagagtcg
     3961 tagcgcactg tggcgacgac aaggccgctt tcttctctgg aagcctcata cgaccagctc
     4021 ccatggtcgc gagttcgaat acggccctta ggttcctttg ccacttgcgt ttttgccgcg
     4081 acagccgtgg cagtcccttc ggcggggagc gccgagagct ttacagagcg ggaagtgggt
     4141 atagcgagca acccggttac gttggcctga gcctggagag cgacctggac gtaagacacc
     4201 gctatgatga aggccaatac caggcacaca attgccacca accacgccaa acgcctcgaa
     4261 agcgttacac acatatagga ctcctttagt cctgggaaac gagggtgcat acagcctgag
     4321 cagcaagcac cctgaccggc aagcatacca atttggggtc ctgacttgct ggtcggcagg
     4381 cccgaacggc gggcacctgc ttgagccgcc gcttcatttg gttgcactac acggttgtag
     4441 ggaagccccg caagtaacgt aacgatacta agctgcccag gttgcacacg ccaccttcaa
     4501 caggcccttt atcccacaag caaaggccgt tgttatatac tagaagatga gccgtaccgg
     4561 aacaaatcgg taacctcttt gaggtccttt ggctgcgcca atgccatgcc tggcgtttta
     4621 ggaatgcaca cccacagcga aggaagtagc ataccatctc caaagaaggg ggactgacat
     4681 ggaaaccatg acgagcctgc gcgcaccacg cggcacgacc ctccagtgca aaggctggct
     4741 gcaggaggcc gccctcagaa tgctgatgaa caacctcgac cccgacgtgg ccgagcgccc
     4801 gcaagacctg gtcgtgtacg gcggcagggg caaggcggcc cgcgactggc cctccctgct
     4861 caagatcctc gactgcctcc gcaacctgga gaacgacgag accctcctgg tgcagagcgg
     4921 caagcccgta ggcgtcttca agacccacac cgacgcgccc cgcgtgctca tcgcaaactc
     4981 caacctggtg ccccactggg ccacgcgaga ggtcttttcc aggctggaag cgcagggcct
     5041 caccatgtac ggccagatga ccgcaggtag ctggatttac atcggcaccc agggcatcct
     5101 ccagggcacc tacgagacct tcggcgcggc tgcccgcaag cactacggcc cggaggccga
     5161 cctgagcggc aagctggtgc tcaccgcggg gctgggcggc atgggcggcg cgcaaccgct
     5221 ggccgtcacc atgaacggcg gagtggccat cgtcgtggag gtggaccaga cccgcatcca
     5281 gcgcagactc gatacccgct acctcgacac cgccaccgac aacctcgaag acgccctgca
     5341 aatagccgag gcagccacca gggagcgccg tcccctctcc atcggcctcc tcggcaacgc
     5401 ggcggcgatt tacccggaga tggtgcggcg cggcagcata cccgacatgg taaccgacca
     5461 gacctccgcc cacgaccccc tcaacggcta cgtgcccctc ggctatgacc tcgatagcgc
     5521 ggcggccctc cggcatagtg accctggtga gtacgtgcga atgtcgctgg aatcgatggc
     5581 cgtccacgtg cgcgccatgc tcgacctgca acgcatgggc gctaccgtgt tcgattatgg
     5641 caacaacatc cgccagcagg cgttcgggca gggtgtgacg gacgccttcg acttccccgg
     5701 cttcgtgccc gcctacatcc gcccgctctt ctgcgagggc aaaggcccct tccggtgggc
     5761 ggccctatcc ggcgatgcgg aggacatcta cacgattgac gatatggtgc tgcgcgagtt
     5821 ccccgacaac gcctccgtgc aacgatggat acccctggcg cgtgagaagg tcgccttcca
     5881 gggcctcccg gcgcgcatct gctggctcgg ctacggggag cgggcgcact tcggccaggt
     5941 tgtcaacgag atggtgcgca ccggccaggt gagcgccccc atcgtcatag gccgcgacca
     6001 cctcgatacc ggcagcgtgg cctcgcccaa ccgcgagacc gaggcgatga aggacggcag
     6061 cgatgcaatc tccgactggc ccatcctaaa cgccctcatc aacgccgtgg ggggcgccac
     6121 ctgggtg
//
LOCUS       DASRWZ010000171         9090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_219214, whole genome shotgun sequence.
ACCESSION   DASRWZ010000171 DASRWZ010000000
VERSION     DASRWZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9090
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219214"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..983
                     /locus_tag="VFH60_02575"
     CDS             54..983
                     /locus_tag="VFH60_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6312694.1"
                     /translation="MPVQVNEKACIDRAHCFAAAACPYDAYFHNSLRKTWEVESTICG
                     DCPGPCLNFCDKDALLWGDDLIDLKLVKAEVDGTMTHAEVVEARAKHKKDLIEAAAAA
                     EAARVAAEAARKKKEGAVMEITIRNFEEEVLRSDTPVAVDCWAAWCGPCKQYSPTFEA
                     VARQYEGIVKFAKLDTEAEPTLSRGLGVQALPTTLLFYKGQLVNAVEGALPAEHLQQW
                     LYQTLAVIRQYATQLDAEAEDAITAAVQNLNLLDGNGNLPPSADDLAGPEVPHAQSGF
                     SAQDLDPRNMSPRPPGKDDDGPGRRTASGLYIP"
     gene            1099..1782
                     /locus_tag="VFH60_02580"
     CDS             1099..1782
                     /locus_tag="VFH60_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6312695.1"
                     /translation="MSTPRKIDAKTAAKRRAARRKQTRETMGRCGIIAIVAILVIGTI
                     ASVIIPGTVTAPGAVPTDVPAVNPTATQPDLSGLQSLVQLGDEAASKGNWADAASIYR
                     SYLAQDPTDGQVHFKLGKVYLSMQPPNYLEALSELQQALSINPSAPYAQEAQSLIDQY
                     KDNALTPSVITGTMTTLPGSITGTVTITSTGTVTGSGTLTSTVVVTGTSAAGTAPPTA
                     PPAGGTQTP"
     gene            2117..3583
                     /locus_tag="VFH60_02585"
     CDS             2117..3583
                     /locus_tag="VFH60_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6312696.1"
                     /translation="MKAEEIDCTTSGEGATGQRSLREVFARDPRDLIVGGRTLVPTLD
                     GTSRPYVNLDSAASTPIARPVKDVVDSFLEWYAAVHRGSGFKSQVSSLVYEQARERLA
                     RFVGADLNDRCLIFVRNATEAINRCANRIPLSEGDVVLTSLMEHHSNILAWRRRCAHV
                     ESVSVDQFGMPILEDLEKRLHANAGHVKLVAISGGSNVTGVIPDVRSIARVTHEAGAV
                     LLVDAAQLAPHRPIEMGTMGDPESIDFLAFSGHKMYAPLGCGVLIGPRSAFEGGIPDL
                     VGGGTVLFVSEEEEMWGGPPDSEEAGSPNVVGAVAMAAAARFLADDLGWEAVVRHERE
                     LTSYALEKLNELPGLTIYGPRDPALPQDRLGVIAFNLEKSHHQLTAAILSHEYAIGTR
                     TGGFCAHPYLMRLFGIGTDSVHTLRDEVSGGDRRNMPGAVRMSFGFYSTRDDVDAIIH
                     ALWDIQRGKWGGDYTQNPKSGEYKPTSELTSPVGWFSI"
     gene            complement(3580..4122)
                     /locus_tag="VFH60_02590"
     CDS             complement(3580..4122)
                     /locus_tag="VFH60_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6312697.1"
                     /translation="MLQSDNAQAPLAPHADRPEMPEGYGIPKSIEGVLPWSYVSDMMS
                     NALNYWIGTTRPDGRPHAVPIWGVWVDESLYFECGPNTRRGRNLAANPAVVVHIERGD
                     EVVILEGTTEQIENPDPSLAERLAEASGAKYGPQYNYQPKPDSRQEGGLCVVHPTVAF
                     AWTKFPEDCTRWRFEKSPGA"
     gene            4313..5575
                     /locus_tag="VFH60_02595"
     CDS             4313..5575
                     /locus_tag="VFH60_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6312698.1"
                     /translation="MNKITLSRRHRAPVWLLLMVLVLPGMLGACSLSSVEVRQSRTTT
                     PPANRQATPVPTQQDSSQNNQGDQGNSSPGGSGGDLNSPPAQQADGSAGAAWQIPAEQ
                     QAVVRVVEQVNPAVVTVVNKLDPRQSGFSGEARGSGVVIDEEGRIITNNHVIEGAVEG
                     GLQVIFIDGTIAPAQLIGADPGNDLAVLKVDTEVKVVATLADSSKVKVGETVIAIGSA
                     LGDFTNPVTVGVVSGTNRDLEGQEVQLENLIQTDAAINHGNSGGPLLNLSGEVIGINT
                     AVVRGDGSGVGAGDVAEGLGFAIPSNRVRTVAGRIIQQEGRPRPFLGVATEPVTPQLA
                     SYYDLRGPNGRLLSSGVLVVEVVPGSAAEDVGLAPGDVILSIDDTTLNEDNPLADVLA
                     EYEAGQTVRLQVVRNGSSQDVDVTLGGR"
     gene            5845..6198
                     /locus_tag="VFH60_02600"
     CDS             5845..6198
                     /locus_tag="VFH60_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6312699.1"
                     /translation="MDILTVEEVAERLKLNPFTIRRLLREGVLQGSKVGKRQWRIQLR
                     DLEAYLSQGSNDGTRPRRGSATMEPVSPQGRHPYPQDFLDVEAADLNAMLARVTPENR
                     HELVDWGARSGNEVW"
     gene            6200..6553
                     /locus_tag="VFH60_02605"
     CDS             6200..6553
                     /locus_tag="VFH60_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET6312700.1"
                     /translation="MALPAQPYVPDRGDLVYLDFNPVRGHEQARVRPALVLSPLSYNS
                     KVGLALVCPITSRAKGYPFEVALPEGLAVSGVILSDQVKSISWPHRSILYKDSLPVQV
                     FRQVIANVLGLLTKG"
     gene            6695..8902
                     /locus_tag="VFH60_02610"
     CDS             6695..8902
                     /locus_tag="VFH60_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312701.1"
                     /translation="MHVLIVFGLALGVRLAYWQDARAYSIGADEPDYVLPAQTLLRDG
                     RYVDTFITPGRTWTRVPLTSLVFASSFLFVPDEAAKQAAGDDAALMEPRYDALNLVQI
                     GESLLTVGLIMLLAGRIFPQKGARAAIVAGYIAALYPPLASSPAQRALSEPTSILFVV
                     AAMYAFSWWAPEAATSKNLLVGIVGGALLGFGALARPIALTFLPFVLVWLFVTYRSAR
                     RSPGAEAGGKPRGVGLFARYRKPLIVSAVATIVALACIAPWTLYNYRQYGRFLLLDTA
                     STTAYWNYHNFRGEDITARMAALPNPADRLSLIISEGTANIVEYPGEAAGSVVFAFFY
                     FWHLESNSAVLLNPWDMTQRDPDVPDLLHADAAFLLVGLMGVCGLAGVGFRRPDDRAG
                     RTLLLSNLWLLSMVLLGLVVPYDGRYRLPAVPSFIVLAAGLLVLADWRAVFSPRRAWA
                     IARAHGRVVVVASALCLWAVMGAYSPNIPPLLRTLYQAWRGDLALQAEDVASAIGRYR
                     LAQQAFPGFFYGYRREAEAARFAGMDDHARQFYATSHELAPDDAYNILGFADLASRHP
                     EWQFTIAERGWLMRDEDVWRGNPWNSFRPTPTRSIDVGSGGDSVYIRGFHRPDQPSPD
                     FNYRWSRGRAVVRVPLPAGAFGAVRLRMSAPGVGPSEPMQVSVTQNVSQPTVLQVAPG
                     WADYNVPIADVPGTTLTLEIASPTRKVADYVPDTNDPRSLGVGIDSVTLVEGP"
BASE COUNT         1689 a         2827 c         2952 g         1622 t
ORIGIN      
        1 tatggctggt gtgtataatg atatagagac gaagagggag gagttttcga gagatgcccg
       61 ttcaggttaa tgagaaggcg tgcatcgaca gggcgcattg cttcgcggcg gcggcatgcc
      121 cttatgatgc ctactttcac aactcgttgc gcaagacgtg ggaggtcgaa tcgactatct
      181 gcggggactg tccaggccct tgcctcaact tctgcgacaa ggacgccctc ctctggggtg
      241 acgacttgat cgacttgaag ttggtgaagg ccgaggtaga tggcacgatg acgcacgccg
      301 aggtggtaga ggcccgcgcc aaacacaaga aagaccttat agaggccgcg gcagccgccg
      361 aagccgcccg ggttgccgcc gaggccgcaa ggaagaagaa agaaggtgca gtaatggaga
      421 taacgatacg caattttgaa gaggaagtat tgaggtcgga tacacccgtg gcggtagatt
      481 gctgggcggc atggtgcggc ccctgcaagc agtactcgcc cactttcgag gccgtagcca
      541 ggcagtacga gggcatcgtg aagttcgcca agctcgatac cgaggcggag ccgaccctgt
      601 cccgcggcct gggcgtgcag gcgctgccca ccaccctgct cttctacaag ggccagctcg
      661 tcaacgccgt ggagggtgcg ctgcccgccg agcacctgca acagtggctc taccagacgc
      721 tcgccgtcat acgccagtac gctacccagt tggacgccga ggccgaggac gcgatcacgg
      781 cggccgtcca gaacctcaac ctgctggacg gcaatggcaa cctgcccccc tccgccgacg
      841 acctggccgg ccccgaagtg ccgcacgcgc agtcaggctt cagcgcccag gacctcgacc
      901 cgcgcaacat gtcgccccgg ccccccggca aggacgacga tggccccggc aggcgcaccg
      961 ccagcggcct gtatatccct tagtgatgag tgacgaggac tgaggactga gtgggaccag
     1021 aacgaactac tcagtcctca tcactcatta ctcattactc gttgaccgtt tcacggctga
     1081 tttggtacaa tcactaccat gtctacaccc aggaagatag acgccaaaac ggcggctaag
     1141 cggcgggctg cccgacgcaa gcaaactcgt gagacgatgg gacgatgcgg tattatcgct
     1201 atcgtggcta tccttgtaat tggcaccata gccagcgtga tcataccggg caccgtcacc
     1261 gcgcccgggg ccgtgcccac tgacgtgccc gccgtgaatc ccacggctac ccaacccgat
     1321 cttagtggtt tgcagtcgtt ggtccagcta ggcgacgagg cagctagcaa gggcaactgg
     1381 gcggatgcgg ccagcatcta caggtcgtac ctcgcccagg accccacgga cggccaggtg
     1441 cacttcaagc tgggcaaggt ctacctcagc atgcagccgc ctaactacct ggaagccctg
     1501 tccgagttgc agcaggcgct cagcatcaac cccagcgcgc cctacgccca ggaagcccag
     1561 tctctcatag accagtacaa ggacaatgcc ctcaccccca gcgtgataac gggcaccatg
     1621 accaccctcc ccggcagcat caccggcacg gttactatca cgtccaccgg cacggtcacc
     1681 ggtagcggca cgctcacgtc cacggtggtt gtgacgggca ccagcgcagc aggcacggcc
     1741 ccgcccacag ccccacccgc cgggggcacg cagacgccgt aaacagcacc ggcttagggg
     1801 tattaagaga cggaaccgcc aagacgccaa ggatgccaag aggattacgg ttagatttta
     1861 caaagaggac caggatgcat atcattgcac cctggtcctc tacttaataa caccgtgact
     1921 actctttgcg cgtcctttgc gcctgtgcgt ctttgcgttt tcgtactctt ggcgttcttg
     1981 gcgtcttggc ggttccgtct agttgctgtt agcggtgcga cacagttgca aaggtgggag
     2041 tgtggcggta tagtatgggg cagctacaga gcatagggag ggcgctcaag ggcgagcgta
     2101 ccggaaggaa tcgaatatga aggctgagga gatagactgt accacgtccg gcgagggagc
     2161 cacaggtcag cggagcctgc gggaggtatt tgcccgcgac ccgcgagacc ttatagtcgg
     2221 cgggcgcacc ctcgtgccta cgctcgacgg cacaagccgc ccctacgtca acctcgacag
     2281 cgccgcctcg acccctattg cccgccctgt gaaggacgtg gtagacagct tcctcgaatg
     2341 gtacgcggcc gtgcacaggg gcagcggctt caaatcgcag gtgtcctccc tcgtgtacga
     2401 acaggcccgc gagcgacttg cccgcttcgt gggggccgat ctcaacgacc gctgcctgat
     2461 cttcgtccgc aacgccaccg aggcgatcaa ccgttgcgcc aaccgcatac ccctttccga
     2521 gggcgacgtg gtgctcacct ccctcatgga gcaccattcc aacatactgg cctggcggcg
     2581 gcgctgcgcc cacgtcgaaa gcgtatcggt ggaccagttc ggcatgccca tcctggaaga
     2641 ccttgagaag cgcctgcacg ccaacgcggg gcacgtcaag ctggtagcca tctccggcgg
     2701 ctcgaacgtc acgggcgtca tccccgacgt gaggagcatc gcccgtgtga cgcacgaagc
     2761 gggcgcggtg ttgctggtgg acgcggccca actcgcgccc cacaggccca tcgagatggg
     2821 cactatgggc gaccccgaat cgatagactt cctcgcgttc tccgggcata agatgtacgc
     2881 gcccctgggc tgcggcgtgc tgatcgggcc gcgctccgcg ttcgagggag gcatacccga
     2941 cctggtgggc gggggcacgg tgctgttcgt gtcggaggaa gaggagatgt ggggcgggcc
     3001 gccggacagc gaggaggcgg gcagtcccaa cgtggtgggg gcggtcgcga tggcggccgc
     3061 ggcccgcttc ctggcggacg acctgggctg ggaggcggtg gtgcggcacg agcgcgagct
     3121 tacgtcttac gcgctggaga agctgaacga gctacccggt ctcactattt acggcccgcg
     3181 cgaccccgcc ctaccccagg accgcctcgg ggtgatagcc ttcaacctgg aaaagtcgca
     3241 ccaccagcta accgccgcga tcttgagcca cgagtacgcc attggcacgc gcacgggtgg
     3301 cttctgcgcc cacccctacc tgatgcggct gtttggtatc ggcaccgaca gcgtgcatac
     3361 cctgcgcgac gaggtgagcg ggggcgaccg ccgcaacatg cccggagctg tgcgtatgtc
     3421 gttcggcttc tacagcaccc gcgacgacgt ggacgccatc atacacgcgc tgtgggacat
     3481 ccagcggggc aagtggggcg gtgattacac gcagaacccg aagagcgggg agtataagcc
     3541 cacctcggag ctgaccagcc cggtggggtg gttcagcatt taggctccgg ggcttttctc
     3601 gaagcgccaa cgcgtgcaat cctcggggaa cttggtccag gcgaaggcga cggtgggatg
     3661 tacgacgcag aggccaccct cctgccggct atccggcttg ggttggtagt tgtactgcgg
     3721 accgtacttt gccccagatg cctcggccag gcgttccgca agcgaggggt ccgggttttc
     3781 tatctgttcg gtggtgccct ccaggattac cacctcgtcc ccgcgctcga tgtgcaccac
     3841 aacagccggg ttcgcggcca ggttgcggcc ccgccgggtg ttcggcccgc actcgaagta
     3901 taacgactca tcaacccaca caccccatat tggcaccgcg tgcggccttc catccgggcg
     3961 tgtggtacct atccaataat tcagcgcgtt gctcatcata tcgctgacgt agctccaggg
     4021 taacacccct tctatgctct tggggatgcc gtagccctcc ggcatctcag gccggtcggc
     4081 gtgcggtgcc aggggtgctt gcgcattgtc actttgcaac atacagagtc ctccctttcg
     4141 tgggttagag ttcgtcttgc ggaatatata cacgagcata cctgacacct gctgtcaggt
     4201 tgccgctaat acccctccgg tggcctaaac tctgcttata ctccttacgc atagatgcat
     4261 ctctttgggc gtgtgcaaat acggcagtca aaccgcagac aggcaggtta gcatgaacaa
     4321 aatcaccctt tcccgcaggc accgggcacc cgtatggttg cttctaatgg tgcttgtgct
     4381 tcctggtatg ctgggcgcgt gcagcctcag tagcgtggag gtgaggcagt cccgcacgac
     4441 gacccccccg gccaaccggc aagccacccc ggtgcccacc cagcaggata gctcgcagaa
     4501 caaccagggc gaccagggta acagcagccc gggcggctct ggtggcgacc tcaactctcc
     4561 acccgcgcaa caggcggacg gctcggcggg ggccgcatgg cagataccgg ccgagcagca
     4621 ggccgtagtc cgggtagtcg agcaggtgaa cccggcggtg gtcacggtgg tcaacaaact
     4681 cgacccacgc cagagcggat tcagcggcga ggcgcgcggc tcaggcgtgg tgatcgacga
     4741 ggagggccgg atcatcacca acaaccacgt gatcgagggc gcggtcgagg gcggcttgca
     4801 ggtgatcttc atcgatggca ccatagcccc cgcccagttg attggggccg accccggcaa
     4861 cgacctggcc gtgctcaagg tggacaccga ggtaaaggtg gtcgccaccc tggctgactc
     4921 cagcaaggtc aaggtaggcg agacggtgat agcgataggc tcggcgctgg gcgacttcac
     4981 caacccggtc accgtgggcg tggtgagcgg caccaaccgc gacctggaag gccaggaagt
     5041 gcagttggag aacctgatcc agacggacgc cgcgatcaac cacggcaact cgggcgggcc
     5101 gctactcaac ctgtcgggcg aggtgatagg catcaacacg gcggtggtgc gtggtgacgg
     5161 gagcggcgtg ggcgcgggag acgtggcgga ggggctgggc ttcgcgatac cgtccaaccg
     5221 cgtcaggacg gtggcgggcc gcatcatcca gcaggagggc aggccgaggc cgttcctggg
     5281 cgtggctacc gagccggtta ccccgcagct cgcctcctat tacgacctgc gcggccccaa
     5341 cggcaggctg ctcagcagcg gcgtgctcgt cgtagaagtg gtgcccggca gcgccgccga
     5401 ggacgtgggg ctggcccccg gcgacgtgat actcagcata gacgacacca ccctcaacga
     5461 ggacaacccc ctggccgacg tgctggccga gtacgaggca gggcagacgg tccggctgca
     5521 agtggtacgc aacggcagca gccaggacgt ggacgtgacg ctggggggaa ggtaattagt
     5581 gacgagtgac gagtgacgag tgacgagggg acgccctgtg gctaaagcca cgggctggga
     5641 agtagcaaag tcccctacgg ggactactag aggcgagaga gggtccgtag cagactttgc
     5701 ctctggtgag cctggcagtt catttgataa ggtggttagt tgccctgctg agaatgataa
     5761 tatcctgctg ggatcctcat agttgtggtt tagtgtgtta ttgtatgcta ttgtgtgcta
     5821 tagtatgtca ggaggtatat gaacatggat atcctgacag tggaagaagt agccgaaaga
     5881 ctgaagctca accccttcac aatccggcgc ttgctgaggg agggtgtgct tcagggtagc
     5941 aaggtaggta aacgacagtg gcgcattcag ctccgcgacc tggaagcata tttgagccag
     6001 ggtagcaacg atgggacgcg cccgcgacgt ggcagcgcta ccatggagcc ggtgtcgccg
     6061 caaggcaggc acccgtaccc tcaagacttc cttgatgtgg aagcagcaga cctgaacgcg
     6121 atgctggcgc gggttacccc ggagaaccgt cacgagttag tagactgggg cgctcgttcc
     6181 ggcaacgagg tctggtaagt tggctcttcc tgcacagcct tacgtaccag accgtggaga
     6241 cctggtgtac ctcgacttca acccagtcag ggggcacgaa caagccaggg tacggccggc
     6301 cctggtgttg tcgcctctca gctataactc caaagtggga ttggcgctgg tatgccctat
     6361 aaccagccgc gcaaaaggct atccgttcga ggtcgcactg ccggaagggc ttgccgtcag
     6421 cggcgtgata ctttccgacc aggtgaagag tatatcctgg ccgcaccgca gtatcttata
     6481 caaggactcg ctgccggtcc aggtgttccg gcaggtaata gctaacgtac tggggttgct
     6541 gacgaagggc tagcttggag gacccggtgt cgcaaggcgg gggagcaggt cagagatggc
     6601 ttgctctttt gtttggtggg tatcctcatt tgtgtgtgct acaataccag cgatgagcaa
     6661 gccacggcca aagtctctat cctgggcgag cgttttgcac gtgctgatcg tcttcgggct
     6721 ggcgctgggg gtgcggctgg cctactggca ggacgcgcgg gcttacagca tcggggcgga
     6781 cgagccggac tacgtgctcc ccgcacagac gctgctcagg gacgggcgct acgtagatac
     6841 cttcatcacg cctgggcgca cctggacgcg ggtgcccctt acctccctgg tgttcgcctc
     6901 ctcgttcctc ttcgtgcccg acgaggccgc caaacaggcc gccggggacg atgccgccct
     6961 catggagccg cgctatgacg cgctcaacct cgtgcagata ggggagagcc tcctcaccgt
     7021 ggggcttatc atgctgctcg cggggcgcat cttcccgcag aagggcgcgc gggcggcaat
     7081 cgtcgcgggg tatattgcgg cgctatatcc tcccctcgcc agcagccccg cccagcgcgc
     7141 gctcagcgag cctacatcga tcctgttcgt agtggcggct atgtacgcct tctcgtggtg
     7201 ggcaccggaa gcagcgacca gcaagaacct gctggtgggt attgttggtg gggctttgct
     7261 ggggtttggc gcgctcgccc gccctatcgc tcttaccttc ctgccgttcg tgctcgtgtg
     7321 gctgttcgtc acgtacaggt cggcgcgcag gtctcctgga gcggaggcgg ggggcaagcc
     7381 tcgcggggtg gggctgttcg cacggtaccg caagccgctg attgtatcgg cggtggctac
     7441 tattgtcgct ctggcgtgca tcgcgccatg gacgctgtac aactacaggc agtacgggcg
     7501 gtttctgctg ctcgacacgg ccagcaccac ggcgtactgg aactaccaca acttccgggg
     7561 cgaggatatc acggcccgca tggcggcgct gcccaacccg gcggaccggc tgtcgctcat
     7621 catctcggag ggcacggcga acatcgtgga gtaccccggc gaggcggcgg gcagcgtcgt
     7681 gttcgccttc ttctacttct ggcacctgga aagcaactcg gcggtgctgc tcaacccctg
     7741 ggatatgacc cagcgcgacc ccgacgtgcc cgacctgttg cacgccgacg ctgctttctt
     7801 gctcgtgggg cttatgggcg tgtgcgggct tgcgggagtg ggcttcaggc ggccggacga
     7861 cagggcgggg cgcaccttgc tgctgtcgaa cctgtggctg ctgtcgatgg tgctgctggg
     7921 gctggtggtg ccctacgatg gccgctaccg cctgcctgcc gtgccctcgt tcatcgtcct
     7981 ggccgccggg ctgctcgtgc tggccgactg gcgggcggtc ttctcgcccc gtagggcctg
     8041 ggccatagcg agggcacatg ggagggtggt tgttgtcgcc tcggcgctgt gcttgtgggc
     8101 ggttatgggg gcctactcac ccaacatacc gcccctgtta cgcacccttt accaggcctg
     8161 gcggggcgac ctggcgttgc aggcggaaga cgtcgcgtcc gcgattgggc ggtacaggct
     8221 ggcgcagcag gcgttccccg gcttcttcta cggctacagg cgcgaggccg aggccgcccg
     8281 cttcgccggc atggacgacc acgcccgcca gttctacgcc acgagccacg agctggcccc
     8341 cgacgacgct tacaacatcc tcggtttcgc ggacctcgcc tccagacacc ccgaatggca
     8401 gttcactatt gccgagcgcg gctggctgat gcgggacgag gacgtgtggc gcggcaaccc
     8461 ctggaacagc ttccggccca cgcctacccg ctctatcgac gtgggttcgg gcggcgacag
     8521 cgtctacata cgcggcttcc accgccccga ccagccctct cccgacttca actaccgctg
     8581 gagccggggc agggcagtcg tgcgcgtccc gctgcccgca ggcgcgttcg gggccgtgcg
     8641 cctgcgcatg tcggcccccg gcgtaggccc aagcgagccg atgcaagtca gcgtcacgca
     8701 gaacgtgtcg caacctacgg tgttgcaggt cgcccccggc tgggcggact acaacgtacc
     8761 tattgccgac gtaccgggca ctacattgac gctagaaatc gccagcccca cgcgcaaggt
     8821 ggccgactac gtacccgaca ccaatgaccc ccgcagcctg ggcgtaggca tagacagcgt
     8881 cacgctggtg gaagggccat aggcggcata ggcgttgcct ccaccggata aggcggattt
     8941 gcgggactca gtcccccttt gtcatcctga gcgtagcgaa ggatcaggtg ggtaggacag
     9001 agtctacaac gctcaggatt agggcggtat gaggaacact ggtgcaacaa tagggagagt
     9061 caagaagggg cggctgatcc ttcgctgcgc
//
LOCUS       DASRWZ010000172         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1041883, whole genome shotgun sequence.
ACCESSION   DASRWZ010000172 DASRWZ010000000
VERSION     DASRWZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041883"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..507
                     /locus_tag="VFH60_02615"
     CDS             133..507
                     /locus_tag="VFH60_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6312702.1"
                     /translation="MTNPLEGASNEHIAPLVEAMRADPQQAAYYLALIEPLRQYDREH
                     HGDLLKTLSTYLRHGGNSVRTANALFIHRNSLRYRLSRVQALTSLNPDDPDARLALQI
                     ALVLNAQLNPQSEIPISKFEEL"
     gene            508..762
                     /locus_tag="VFH60_02620"
     CDS             508..762
                     /locus_tag="VFH60_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET6312703.1"
                     /translation="MNEPAPEAVALTSARNKLPGVITRVKRGDVMCQVDMQVGDNHIV
                     AIITTEAADEMGLEPGMQAVAMFKSTSVMIATPGTHDSGL"
     gene            1151..2839
                     /gene="modA"
                     /locus_tag="VFH60_02625"
     CDS             1151..2839
                     /gene="modA"
                     /locus_tag="VFH60_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6312704.1"
                     /translation="MPLAALAQTPQPGAATFADPTFERTWQRTDKPVADGTVKRGFYW
                     GPAPFDKRQEQYAEGQGGTRLVQYFDKSRMEINDPNGDKNSPFYVTNGLLTVELVSGR
                     MQTGNNSYTDRTPANIPLASDNDDPTAPTYASFAKLLADSGNLTNQNVTATVDKAGSV
                     GQDAALGSTAGAKLVYYEAQTKHNIPQVFWDFLNVKGPVYESGALATKQISEPWFYAS
                     GLPITEPYWARVKIAGKADTLVMVQLYERRVLTYVPSEPQPEFRVQVGNIGRHYHDWR
                     YAEGSQPGLPIAPPALPTEPGPPTIPPPPRTVQPTLAPTNLNVFGASSLKESFEEMGQ
                     KFKAANRNLRELSFNFQGSQSLVAQMQQGAPADVFTSADRANMDKAVAAGLVSGTPRA
                     LARNLLTVVLPNENFGNITSLKDLAKPGVKLSIADPSVPVGKYSLEALDKLSADPAYG
                     AGFKQAVLNNVVSRENNVRQVLARVQLGEVDAGIVYVTDARAGNASTPGGVPPVKTLA
                     IPEKYNVAAIYYIAAVKGAAHPEAGRAFIGYALSAEGQAVMEKYGFSRANATRP"
     gene            2855..3745
                     /locus_tag="VFH60_02630"
     CDS             2855..3745
                     /locus_tag="VFH60_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6312705.1"
                     /translation="MAPGTGQEHQSNNATPAQSRAYPRPAEAAWGPLLGRLAVGMASA
                     VCLAFLTLPLLALLLRVPLPDLPRYLTNPLVLDALRLSLISSLCSLALMALLGTPVAW
                     GLARSNFPGKRVVETLVELPLVLPPAVAGVALLFAFGRRTPVGGVLRDLNIEVAFTLA
                     AVVMAQAFVAAPFYIKSARAGFQSVPRELVEAAQTQGAGGWQTFRHIVVPLALPSIAG
                     GAVMGWARAVGEFGATILFAGNFQGRTQTMPLAIYTALETDINAAVVLAAILVVSSFA
                     LLITFKLLSGRQLDVVGLGE"
BASE COUNT          750 a         1357 c         1199 g          617 t
ORIGIN      
        1 gccactccga ttctgtgcac tttgcactaa caccgcccgg aggtgtgtgc tacaacatat
       61 atagtagcac tctcagacaa aggaccacag gcacacccgc cgtccatcgt ccatagtcca
      121 ttggaggtac gcgtgaccaa ccccctcgaa ggggcatcca acgagcacat agcgccgctg
      181 gtcgaggcga tgcgggccga cccacagcag gcggcctact acctggccct gatagagcct
      241 ctgcgccagt acgaccgcga gcaccacggc gacctgctca agacgctgtc cacgtacctg
      301 cggcacggcg gcaactcggt gcggacggcc aacgccctct tcatccaccg caactccctc
      361 cgctaccgcc tctcccgcgt ccaggccctc acctccctca accccgacga cccggacgca
      421 cgcctcgctc tacaaatcgc cctggtcctg aacgcacagc taaatccgca atccgaaatc
      481 ccaatttcca aattcgagga gctttagatg aacgaaccag cccccgaagc cgtggccctc
      541 accagcgctc gcaacaagct gcccggcgtt atcacccgcg taaagcgcgg cgacgtgatg
      601 tgccaggtgg acatgcaggt gggcgacaac cacatcgtcg ccatcatcac cacagaggcc
      661 gccgacgaaa tgggcctgga gccaggcatg caagccgtcg ccatgttcaa atcaacctcc
      721 gtcatgatcg ccacccccgg cacgcacgac tcgggcttgt agcgggacga tggacgatgg
      781 atgatggcag ttgccttatc atgtatcggc tgtgcctcag tagatgtgac aggaccagag
      841 tccccctctg tcatcctgag cgtagcgaag gatctaaaga aggtggtaca gggttgtaat
      901 tgaacatcag ggatcatggt cgtgaaagtt gaacgtcggg gcagaggtag catcttagat
      961 ccttcgctac gctcaggatg acagggggga gtgtggtcgc cgccattgat acgtagtctc
     1021 tatcggccca gcatcgtcca tcgtccacct ccaagaaagg aagttacatc tacacatgcg
     1081 tcttttcaca acatcgcagc acaacttcaa gagaacccta tttggcctgt caatcgcggc
     1141 gctcgcctta gtgcctttag ccgctctggc gcagacgcca cagccggggg ccgctacctt
     1201 cgcggacccc accttcgagc gcacctggca gcgcaccgac aagcccgtag ccgacggcac
     1261 ggtcaagcgc ggcttctact ggggtccggc gcccttcgac aagcggcagg agcagtacgc
     1321 cgagggccag ggcggcacgc ggctggtgca gtacttcgac aagagccgca tggagataaa
     1381 cgaccccaac ggcgacaaga acagcccctt ctacgtcacc aacggcctgc tcacggtcga
     1441 gcttgtgagc ggcaggatgc agaccggcaa caacagctac actgaccgca cgcccgccaa
     1501 tatccccctc gcttccgaca acgacgaccc caccgcgccc acctacgcta gcttcgccaa
     1561 gctgctggcg gacagcggca acctcaccaa ccagaacgta accgccacgg tggataaggc
     1621 gggcagcgtg gggcaggacg cggcgctggg cagcaccgcg ggggcgaaac tggtctacta
     1681 cgaggcgcag accaaacaca acatcccgca ggtcttctgg gacttcctca acgtcaaggg
     1741 gccggtgtat gagagcggcg cgctcgccac gaagcagatt tccgagccgt ggttctacgc
     1801 cagcggcctg cccatcaccg agccgtactg ggcgcgggtg aagatcgcgg gcaaggcgga
     1861 caccctggtc atggtgcagc tttacgagcg gcgcgtgctc acttacgtcc cctcggaacc
     1921 ccagccggag ttcagggtgc aggtgggcaa catcgggcgg cactaccacg actggcgcta
     1981 cgccgagggt tcccagccgg gtctacctat cgctccgccc gccctgccga ccgagcctgg
     2041 tcccccaacg ataccgccgc cccctcgtac cgtgcaaccg accctcgcgc ccaccaacct
     2101 caacgtgttc ggggcttcct ccctcaagga gtcgttcgag gagatggggc agaagttcaa
     2161 ggcggccaac aggaacctgc gcgagcttag cttcaacttc cagggctcgc agtcactggt
     2221 cgcacagatg cagcagggcg cgcccgccga cgtgtttacc tccgccgaca gggccaacat
     2281 ggacaaagcc gtagcggcgg gcctcgtcag cggcaccccc cgcgcccttg cccgcaacct
     2341 gctcaccgtg gtgctgccca acgagaactt cggcaacatc acgagcctga aagacctcgc
     2401 aaagcccggc gtcaagctct ccatagccga cccttccgtg ccggtgggca agtacagcct
     2461 cgaggcgctc gacaagctgt cggcggaccc ggcttacggc gcgggcttca agcaggcagt
     2521 gctcaacaac gtggtcagcc gcgagaacaa cgtgcgccaa gtgctcgcgc gggtgcaact
     2581 gggcgaggtg gacgcgggca tcgtctacgt caccgacgca cgggccggga atgcctcgac
     2641 ccccggtggc gtgccccccg tcaagacgct ggcgataccg gagaagtaca acgtggcggc
     2701 catctactac atcgccgccg tcaagggtgc ggcccacccg gaagcgggca gagccttcat
     2761 aggctacgcg ctgtccgccg aagggcaggc ggtcatggag aagtacggct tcagccgcgc
     2821 caacgcgacc aggccataga gcacgcgggg cagcatggca ccaggaacgg gccaggagca
     2881 ccaatcgaat aatgcgaccc ctgcacagtc cagggcctac ccacggcccg ctgaggccgc
     2941 ctgggggccg ctgctgggca ggctggccgt cggcatggcg agcgcggtgt gcctggcgtt
     3001 cctcaccctg cccctgctgg ccctcctcct gcgcgtgccc ctgcccgacc tgccccgcta
     3061 cctcaccaac cccctggtgc tggacgcgct tcgcctgagc ctgattagca gcctgtgcag
     3121 cctcgccctc atggcgctgc tgggcacgcc ggtagcgtgg gggctggccc gctccaactt
     3181 ccccggcaag cgcgtggtcg agacgctggt ggagctgccc ctggtgctcc ccccggcggt
     3241 ggcgggcgtg gcgctgctgt tcgcgttcgg ccgccgcacg cccgtcggag gcgtgttgcg
     3301 cgacctcaac atagaggtgg ccttcaccct ggcggctgtg gtcatggccc aggcgttcgt
     3361 ggcggccccg ttctacatca agtcggcgcg ggcggggttc cagtcggtgc cgcgcgagct
     3421 ggtggaggcc gcgcagacgc agggggcggg cggctggcag accttccggc atatcgtcgt
     3481 gcccctggcc ctgccgagca tcgcgggcgg cgccgtcatg ggctgggcgc gggccgtggg
     3541 cgagttcggg gcgactatcc tgttcgcggg caacttccag ggccgcaccc agacgatgcc
     3601 cctcgccatc tacaccgccc tcgaaaccga catcaacgcc gccgtcgtgc tggccgccat
     3661 cctggtcgtc tcctccttcg ccctccttat caccttcaag ctgctgtcgg ggcggcagtt
     3721 ggacgtagta ggcttgggtg agtgatttag agacgaaaac gccaagcctg ccctgagcgc
     3781 agccgaaggg acgccaagga cgccaaggac acgccaggtt aagtacggtt caccgcagag
     3841 acgcagagga cgcagaggac gcgctgagat ggtgattgtt cgggaggggg tgagggttac
     3901 acccccagtg tacagtgttg ccc
//
LOCUS       DASRWZ010000173         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1179144, whole genome shotgun sequence.
ACCESSION   DASRWZ010000173 DASRWZ010000000
VERSION     DASRWZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179144"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /locus_tag="VFH60_02635"
     CDS             <1..2008
                     /locus_tag="VFH60_02635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6312706.1"
                     /translation="GRALSAGLSLLAGFMLFSLVRVAAGTRAALYALLLFSVAPFSIV
                     MGQHFSPAALLLLVQAAAVWSLLNWRASVAEQRRQGSAWAFGFAITLGILAALLDPGS
                     VFLAVPAAYVVLAPRSDGGQLLLARRPSATVNTWRDAWAQSANKGKLVGYACALAGAA
                     FLWWLLSSSMDSGLSLAVGDGGGGASSAVAALLSGGTYVQMVGLVIEKVLTVAGMLLL
                     LAGALQGARPPVQFAVHAWLAGGVLHVMSDASRLGRHDDVLLPILLPLCALVGVGAAW
                     AGSLPARVWLAVTEQRRENASDYAVSPHTSWLLDLPEERVEAATPARPQAQVALGKSI
                     AMRAQRAGSRARRVWFIGVGHLAVLAGFAVIALAGSQTATARLQPTPQSLELAATGRE
                     VAAITNLGDRLIVAGPAAPSLFFASARTGWAIPEDDFSIAEVQSLQRQGAAYLLSLDQ
                     EWLGHHADYTGLLTNYTVKKLARNYILFDLNTKPAANDRQYFLESGHTLGGEFRRFWE
                     AHGGVAKLGYPISEELDEASPLDGVTRTTQYFERAVLEYHPEFAGTPNSVMLASVGRW
                     VMSGRELPRVAPFQSTPTRVYFAETGHSLKEAFLRYWQREGGLAMFGYPISEELPEIS
                     PADGKVYTVQYFERARFEWHPTDAGTPKEVPLGLIGKQALEMRK"
BASE COUNT          332 a          697 c          680 g          432 t
ORIGIN      
        1 gggccgggcg ctctctgcgg gcttgagcct ccttgcgggt ttcatgctct tttcgctcgt
       61 tcgggtcgcg gctggtaccc gcgctgccct gtatgcgctg ctgcttttca gcgtcgcgcc
      121 tttcagcatc gtcatgggcc agcacttttc acccgccgcc ctcttgctgc tcgtccaggc
      181 cgccgccgtg tggtcgctgc tcaactggcg cgcgtctgtt gccgagcagc gcaggcaagg
      241 gtccgcctgg gcattcgggt tcgccattac gttgggtata cttgcagccc tgcttgatcc
      301 tggcagcgtc tttctggccg tccccgccgc ttacgtggtg ctggccccgc gttccgatgg
      361 cgggcaactg ctgctggccc gccgcccatc ggcaacggtc aatacctggc gggatgcctg
      421 ggcgcaatct gctaacaaag gcaagctggt aggatatgcc tgcgctttag ccggcgcagc
      481 ttttctatgg tggctgttgt cttcgagcat ggactccggc ctctctctgg ctgtgggcga
      541 tggaggaggc ggcgcgtcat cggctgtagc cgccctgctt agcggcggca cttacgtcca
      601 gatggtgggg ttggtgatag aaaaagtgct gaccgtcgcg ggcatgctac tgttgctggc
      661 aggtgccttg cagggtgccc gccctcctgt gcagtttgcg gtccacgcct ggctcgcggg
      721 cggcgtgctc cacgtcatgt ccgatgcgag ccgtcttggc aggcacgacg atgtgctcct
      781 gcccatttta ctgcccctgt gcgccctggt gggagtgggc gcggcttggg caggctccct
      841 cccggcgcgc gtctggctgg ccgtcaccga gcagcgccgc gaaaacgcct cggactacgc
      901 ggtgtcgccc catacctcct ggctgctgga cctgccggaa gagcgcgtcg aggccgctac
      961 cccggcccgg ccccaggctc aagtagccct gggtaagagc attgcgatgc gggcgcaacg
     1021 ggcaggttcc agggcgcggc gcgtgtggtt cataggcgtg ggccacctcg ccgtgcttgc
     1081 tggctttgcc gtcatagccc tggcaggctc gcagaccgcc acagcccgcc tccaacccac
     1141 cccgcaatcg cttgagcttg ccgcgacggg ccgtgaggtg gccgctatca cgaacctcgg
     1201 tgaccgtctc atagtggccg gacccgcagc gccctccctc ttcttcgcgt cggcgcgcac
     1261 cggctgggcc atccccgaag atgatttcag catagccgag gtgcagtccc tccaacgcca
     1321 aggcgcggcc tacctactca gtcttgacca ggaatggctg ggccaccatg ccgactatac
     1381 aggtctcctc accaattaca cggtcaagaa gctggcgcgc aactacatcc tgttcgacct
     1441 gaacaccaag cctgccgcca acgataggca atatttcctg gaatcgggac atacgctggg
     1501 aggcgagttc cgccgcttct gggaggcgca cggcggcgtg gcgaagctgg gctatcctat
     1561 ctccgaagag ttggacgaag caagccctct cgacggcgtg acgcgcacca ctcaatattt
     1621 cgagagggcc gtgctcgaat accaccctga gttcgctggc acgcccaatt cggtgatgct
     1681 ggcctcggtt ggccggtggg tcatgtccgg gcgcgaattg ccgcgtgtcg cgccgttcca
     1741 gagcacgccc acccgcgtct actttgccga gacgggtcat agcttgaaag aagccttcct
     1801 gcgctactgg cagcgtgagg gcgggcttgc catgttcggc tatcccatct ccgaggaact
     1861 ccctgagatc agcccggcgg atggcaaggt atacaccgtg caatacttcg agcgcgcccg
     1921 cttcgagtgg caccccacag acgcgggcac cccaaaggag gtcccactag gcttgattgg
     1981 caaacaggcc ctggaaatgc gaaaataggg ctgccgtaac ccattcgagt agactataat
     2041 agtcgggttg agtttgcata caggagacgt tgcgcctttg agcctggtca cgcaggacgc
     2101 tgccggactc gtggatagtg tggaagtggg cagcccggcc c
//
LOCUS       DASRWZ010000174         4216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1179155, whole genome shotgun sequence.
ACCESSION   DASRWZ010000174 DASRWZ010000000
VERSION     DASRWZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179155"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFH60_02640"
     CDS             <1..333
                     /locus_tag="VFH60_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6312707.1"
                     /translation="WVLDRGIRWMAIDAISTDHPFNTKVRVARPDLIPEVEAKIGMSM
                     DEAFPWMEDYQCTHTLLFPRGVFHAENVGGQVDEVLDQRVWLGCFPFRFKGGEAAFAR
                     IVAFVQES"
     gene            421..1296
                     /locus_tag="VFH60_02645"
     CDS             421..1296
                     /locus_tag="VFH60_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET6312708.1"
                     /translation="MKPTPFEYYAPRTRQEALDLLAEHAGEAKVLAGGQSLVPSMNFR
                     LAQPSILVDLNGVDDLFGIQVGEDGRLLVGAMTRHRSMERSPIVAERAPLIAEAMPFV
                     AHPQIRNRGTFGGSLAHADPAAELPALAVVLDAQMRVQNATGDRWIGASDFFLGLFTT
                     ALEEDEMLVEVSVPPLPARTGTAFEEFARRHGDYALVGAAAVVTLGEDGTVTESRLVL
                     LSVGDMPVVSMSASAALLGQRPTEEAIRAAAEALEADIDPQADIHATAEYRRHLAKVL
                     ARRTLTLAARRAGGA"
     gene            1306..1833
                     /locus_tag="VFH60_02650"
                     /pseudo
     CDS             1306..1833
                     /locus_tag="VFH60_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
     gene            1970..>4216
                     /locus_tag="VFH60_02655"
     CDS             1970..>4216
                     /locus_tag="VFH60_02655"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6312709.1"
                     /translation="MIGARVKRNEDPRLLTGQALFVDDVHIPGMLHVAFKRSDYAHAR
                     LLSVDVSGAREVPGVVAVYTAEDLGDYWQPSPLLVPPPPAQYVVFNQRTAGQLARDKV
                     RYVGEPVVMIVAESRYVAEDAADAVLIEYEPLDAVVRVEAGLDEDAPLVHEDVGSNMA
                     AHVIQTKGDYASARANADLVVTRHFDYDRGTAAPIETRGVVAQWDQLARHLTVWDSTQ
                     APIPIRNGLAGMLGLSERQVRVVAPFVGGGFGPKIMMFYPEETLVPWAAIKLNRPLKW
                     IEDRLENFVAMTHERGQVHEAELALTKDGKILGIKDVFLHDNGAYDPYGLTVPLNTQT
                     HLLGPYIVPHYYTEFKAVFTNKIIVTPYRGAGRQHGVFVIERLLDAAAKELGIDPLEL
                     RRRNFIHPDQFPYNNVIIAQDFTSLTYDSGNYEPVLDRARAMIGWDEFRAVEGPRLRA
                     EGKRIGIGLVMYVEGTGIGPYEGARIQVEPTGKVTVVTGVGTQGQGHYTVFGQIAAHM
                     LGVPVEDVLVSTGDTGQFNWGTGTFASRGATVAGSAVHAAAGMVAEKAKRLAAEMMEA
                     ADEDIELQGGYAQVKGMPGARVSLSELAARANPLRGAVKPGTEPGLEATAYFGPPMQA
                     TACGAHAIILEVDDETFDVKVQRYVVVHDCGTVLNPLLLEGQVHGGVAQGIGNAFFEQ
                     LVYDESGQLLNASFMDYLLPTSTDVPRVEVGHEETPSPLNAMGVKGAGEAGAIPVGPL
                     YAQALDNAF"
BASE COUNT          760 a         1278 c         1452 g          726 t
ORIGIN      
        1 tgggtgctcg acaggggcat ccgctggatg gcgattgacg ctatctccac cgaccacccc
       61 ttcaacacca aggtgcgtgt ggcccgcccc gacctcatac cggaggtaga agccaagatc
      121 ggcatgagca tggacgaggc gttcccctgg atggaagact accagtgcac ccacaccctg
      181 ctcttcccac gtggtgtctt ccacgccgaa aacgtgggcg gtcaggtgga cgaggtgctg
      241 gaccagcgcg tctggctcgg ctgcttcccc ttccgcttca agggtggcga agcggccttc
      301 gcccgtatcg tcgccttcgt acaggaaagc taggcgagtt tggaatttcg aaattcgaat
      361 ttcggattta gttgatatcc gaacaaattc gaatttcgaa atcccaaatc ccaaattctc
      421 atgaagccta ctccattcga gtactacgcg ccgcgcacgc ggcaagaggc gttggacctg
      481 ctggccgagc acgctgggga ggccaaggtg ctggcgggcg ggcagagcct ggtgcccagc
      541 atgaacttcc gcctggccca gccctctatc ctggtggacc tgaacggggt ggacgacctg
      601 tttggcatcc aggttggcga ggacggcagg ttgctcgtgg gcgcgatgac ccggcaccgc
      661 agcatggagc gcagccctat cgtggccgag cgcgcgccgc tcatagccga ggcgatgccg
      721 ttcgtcgctc acccgcagat acgcaaccgg ggcacgttcg gcgggtcgct ggcccacgcc
      781 gaccccgccg ctgaactgcc cgccctggcc gtggtgctcg atgcccagat gagggtgcag
      841 aacgcgaccg gcgaccgctg gataggcgcg tcagacttct tcctgggcct gttcaccacg
      901 gcccttgagg aggatgagat gctggtggag gtgtcggtgc cgcctctgcc cgccaggacg
      961 ggcaccgcct tcgaggagtt cgcgcgcagg cacggcgatt atgccctggt gggtgcggcc
     1021 gccgtggtca cgctgggcga ggatggcacc gtgaccgaga gcaggctggt actcctgtcg
     1081 gtgggcgaca tgcccgtcgt gtcgatgtcg gcctccgctg ccttgctggg acagcgcccc
     1141 accgaggagg cgattcgggc ggcggcggaa gccctagaag ccgatatcga cccgcaggcc
     1201 gacatccacg ccacggctga gtaccggcgg cacctggcga aggtgctggc gcggcgcacc
     1261 ctcacgctgg cggcacggcg ggcgggcggg gcttgaggaa gcacgatgga ccacgttgtt
     1321 gacgtagaag tgaacggccg gggctatacc cgtgaagtcg agccgcggct gctgctcagc
     1381 gatttcttgc ggcacgagct tgggctgacc ggtacccacg tgggctgcga gcacggcgta
     1441 tgcggggcct gcaccgtgct actcgatggc gtgcaggtgc gctcgtgact cacctttgcg
     1501 gtgcaggcgg ccgggcgcag cgtgatgacc gtggagggcc tgtcgggcga ccccgacgac
     1561 ttgcacccgc tgcaatcagc cttccacgaa gcccacggcc tgcaatgcgg cttctgcacc
     1621 cccggcttcc tgatgaccct gctgcccttc ctcgaagaaa acgacgaccc caccgaacaa
     1681 gagatacgcc tcgccctctc cggcaacata tgccgctgca ccgggtacca gcacatagta
     1741 gacgccgtca cgctggccgc cgccaaacgc cgcgccggca taactacacc cgcccaggaa
     1801 gcggacgtag agggagaagc aggcatgctg taggggaatt cttaattctc aattcttaat
     1861 tcttaattgg gttggtgact atgtgtattc tgttgcgacg ttgttcacgg cagacctaat
     1921 taaggattaa gaattaagaa ttaagaattc tccccgaaag gggagcgaga tgattggtgc
     1981 gcgtgttaag cggaatgagg accctcggct gctgactggg caggcgttgt ttgtggacga
     2041 cgtgcatatt ccggggatgt tgcacgtggc gtttaagagg agcgattacg cgcatgcgcg
     2101 gctgttgagc gtggacgtgt cgggggcgcg cgaggtgccg ggcgtggtgg cggtctatac
     2161 ggccgaggac ctgggggact actggcagcc ttcgcccttg ctggtgccgc cgcccccggc
     2221 gcagtacgtg gtcttcaacc agcggaccgc ggggcagttg gcgcgggaca aggtgcgcta
     2281 cgtgggcgag cctgtggtca tgattgtggc cgagagccgg tacgtggcgg aggacgcggc
     2341 ggatgcggtg ctgatagagt acgagccgct ggatgcggtg gtacgcgtgg aagcagggtt
     2401 ggacgaggac gcgccacttg tgcacgagga cgttggctcg aacatggccg cgcacgtgat
     2461 acagaccaag ggcgattacg cttccgcccg tgccaatgcc gacctcgtag tgacgcgcca
     2521 tttcgactac gaccggggca cggcggcacc catcgagacg cggggggtcg tggcgcagtg
     2581 ggaccagctt gcccgccatc tcaccgtgtg ggactcgacc caggcgccca taccgatacg
     2641 taatggtctc gcggggatgc tcggcctctc ggagcggcag gtgcgggtgg tcgcgccttt
     2701 cgtgggtggc gggttcggcc ccaagatcat gatgttctac ccggaggaga cgctggtgcc
     2761 gtgggcggcg attaagctga accggcccct caagtggatt gaggacaggc tggagaactt
     2821 tgtcgccatg acccacgagc gggggcaggt gcacgaggcc gagctagccc tcaccaaaga
     2881 cggcaagata ctgggcataa aggacgtctt tctgcacgac aacggtgcgt acgaccccta
     2941 cggcctcacc gtgcccctca acacgcagac ccacctgctc ggcccctaca tcgtgcccca
     3001 ctactacacc gagttcaagg cggtgttcac caacaagatc atcgtcaccc cctaccgggg
     3061 tgcgggacgc cagcatggag tgttcgtgat agagcgcctg ctcgacgcgg cggccaagga
     3121 gcttggtatc gatccgctgg agctgcgccg ccgcaacttc atccaccccg accagtttcc
     3181 gtacaacaac gtgattattg cccaggactt caccagcctg acctacgaca gcggcaacta
     3241 cgaaccggtg ctcgacaggg ccagggccat gataggctgg gacgagttcc gcgccgtgga
     3301 ggggccgcga ctgcgcgccg agggcaagcg gattggtatc gggctggtca tgtacgtcga
     3361 gggcacgggc atcgggccgt acgagggggc gcgcatacag gtggagccta cgggcaaggt
     3421 gacggtggtc acgggggtgg gcacgcaggg gcaggggcac tataccgtgt tcggccagat
     3481 agccgcgcac atgctgggcg tgccggtaga ggacgtgctc gtctccaccg gggacacggg
     3541 tcagttcaac tggggcacgg gcaccttcgc gagccgaggg gccacggtgg ccgggtccgc
     3601 ggtacacgct gccgccggta tggtggcaga gaaggccaaa cgtctggcgg cagagatgat
     3661 ggaggccgcc gacgaggaca ttgagctaca ggggggctac gcgcaggtga agggcatgcc
     3721 gggcgcgagg gtgagcctgt cggaacttgc agccagggcc aacccgttgc gtggcgcggt
     3781 caagccgggc accgagccgg gcctggaagc caccgcctat ttcggcccgc ccatgcaggc
     3841 taccgcgtgt ggcgcgcacg ctatcatcct ggaagtggac gacgagacgt tcgacgtgaa
     3901 ggtgcaacgc tacgtggtgg tgcacgactg cggtacggtg ctcaacccgc tcctcctgga
     3961 ggggcaggtg catggtggcg tggcgcaggg catcggcaac gccttcttcg agcaactggt
     4021 atatgacgaa agcgggcaac tgctcaacgc ctccttcatg gactacctgc tccctacctc
     4081 taccgacgtg ccccgcgtcg aagtcgggca tgaagagacg ccttcgccgc tgaacgctat
     4141 gggcgtgaag ggtgcgggcg aagcgggggc tatcccggtc ggtccgctct atgcccaggc
     4201 gctcgacaac gccttt
//
LOCUS       DASRWZ010000175         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1042119, whole genome shotgun sequence.
ACCESSION   DASRWZ010000175 DASRWZ010000000
VERSION     DASRWZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042119"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..991
                     /locus_tag="VFH60_02660"
     CDS             242..991
                     /locus_tag="VFH60_02660"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6312710.1"
                     /translation="MIPLGDEGTPMRRGFPIVNVTIIVLNILVYFLQMTGGDPITYGW
                     SLIPKEIMTGVDLTTPQFLPGVGEVPMYPAPLGIPYLTLFTSMFMHGGLLHIGSNMLF
                     LFIFGDNVEDNMGSLKYLVFYLLCGLGADFLQIFLGGTDSVIPNLGASGAIAGVLGAY
                     LILFPQARVRALIPLGYFMTVAAVPAIIMIGIWIVTQFISVFVAGEQAAGGGGGVAYW
                     AHIGGFIAGAILVFVFRDRSQSNTVLSRFGG"
     gene            complement(1176..>3139)
                     /locus_tag="VFH60_02665"
     CDS             complement(1176..>3139)
                     /locus_tag="VFH60_02665"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HET6312711.1"
                     /translation="PEQPSYPPSAPTQPYTPTPQGGYQAPPEQPGYTPQYGAPQQPQQ
                     YQQYQQPPAGPTAPYVPPQQVNVAPAKKGGRGPVIGLLVAALLLAAGGAAAFFILGRT
                     VNRPAAAVSRVLPPNALAFLSVDPVLEGTQKAAMDQLGEAFKSQPGFDDAWAKITEQT
                     TEMAGGGEAAENCAGGMTDFGSMSSYLGNNLTIAVLPPSSTDLEGLQTGDEEIGAVLS
                     RNVVGMVDLDFNPLNKQGPAAEFKTVTDNVSQAELVEKYRDMDIRKATVCDATLYFTL
                     LDGSATAVLAADAAPLKVVMDQYKDDKGLKTDARFTALQAQVPAERIATLYLNWTEIY
                     KQAGFIDPTATEALQGVEGAMLMTLSAENDGLRLDVASETDFTNNMLGTSMDVQLNAN
                     ARPDVNTLSDIPTGSLAFLLGTDLQTILQGGIDAIRNSGGDDANAGLDDMIAQVQDRT
                     GMDLEQDILPLMSGDWAISVFPDPAAGAFPVRGVVFQMKLKQEDMERAQQVVEALAQG
                     ATDGGSETVDVSGNTFYTLSPDGAALMGTTNDRLIVVVSANGEPADQAAADTADGLGQ
                     GIGSTDAWRDTAKHLPQNSNLIGWLDFNSIRELLEGTLEGEGLDEYETTAAPFVRPIK
                     YLLMGSSSQAPEGDRLSRNHTIFFLGISK"
BASE COUNT          535 a          985 c          969 g          650 t
ORIGIN      
        1 gaatctcgtc ctccaccggg atgttacacc tttcttgaac gcaggtgctc accaatagaa
       61 aaatggtaat tgggtgctga aggacgagat tcttcgctac gctcagaatg acaaaggcgg
      121 cgttagtcct ttaaaagcca cttcttagca gccacgtaat attagtaaca cgccactgac
      181 cactgaccca tcgttctcta accaacactc agactacaca acctacaaag gagaccaacg
      241 catgataccg ttaggcgacg aaggcactcc catgcgccgg ggcttcccca tcgtaaacgt
      301 caccatcatc gtgctcaaca tcctggttta cttcctccag atgaccgggg gcgaccctat
      361 cacctacggt tggagcctca tccccaagga gattatgaca ggcgtggacc tgaccacacc
      421 ccagttcctg cccggcgtgg gcgaggtccc catgtatccc gcgccgctcg gcatacccta
      481 tctgacgctg tttacctcga tgttcatgca cggcggcctg ctgcacatcg gctccaacat
      541 gctcttcctg ttcatcttcg gtgacaacgt cgaggacaac atgggttcgc tcaagtacct
      601 cgtcttctac ctcctgtgcg gcctgggcgc cgacttcctg cagattttcc tcggcggcac
      661 cgactcggtc atccccaacc tgggggcctc gggcgcgata gcgggcgtgc tgggggctta
      721 tctgatactc ttcccccagg cgagggtgcg ggcgctcata ccgctcggct atttcatgac
      781 cgtagccgcg gtgcccgcca tcatcatgat cggcatttgg atagtgacgc agttcatcag
      841 cgtattcgtg gcgggcgagc aggcagcagg tggcgggggc ggcgtcgcct attgggcgca
      901 catcggcggc ttcatcgcgg gggccatcct cgtattcgtc ttccgcgacc gctcccaatc
      961 caacaccgtc ctctcccgct ttggtggctg atttaagatg aaaccgcaaa ggcgcaaaga
     1021 gcgcaaagtt taaggattgt ccagaaggta caaaaaggac cagggtacgg gatatcgtac
     1081 cctggtcctt ttgtttagaa cacctactat atcgttccgt gtcctttgcg ctctttgcgc
     1141 ctttgcggtt tcatctcctc cactagttac gacgcttact tgctgatgcc gaggaagaag
     1201 atggtgtggt tgcggctgag cctgtcgcct tccggtgcct ggctggacga acccatcagc
     1261 aggtacttga tggggcggac gaacggcgcg gcggtggtct cgtactcgtc aagcccctcg
     1321 ccttcgagcg taccctccag aagctcgcgg atgctgttga agtcgagcca gcctatcagg
     1381 ttgctgttct gcggcaggtg cttagccgtg tcgcgccacg cgtcggtgct accgatgccc
     1441 tggccgaggc cgtcggcagt gtccgccgct gcctggtcgg caggctcccc gttggcgctg
     1501 acgaccacga tcaggcggtc gttggtcgtg cccatgagcg cggcgccgtc ggggctgagg
     1561 gtgtagaagg tgttgccgct aacgtccacc gtctcggacc cgccatcggt ggcaccctgg
     1621 gcgagcgcct ctaccacctg ctgcgccctc tccatgtcct cctgcttgag cttcatctgg
     1681 aagaccacgc cccgcacggg gaacgcgccc gcggcaggat cgggaaatac actgatagcc
     1741 cagtcgccgc tcatgagagg cagtatgtcc tgttccaggt ccatgcccgt ccggtcctgc
     1801 acctgtgcga tcatgtcatc caggcccgcg ttagcatcat cgccgccgga gttgcgtatc
     1861 gcatcgatgc cgccctggag gatggtctgg aggtcggtgc ccaacaggaa ggcaagagag
     1921 ccggtcggaa tgtccgagag ggtgttcacg tccggcctgg cgttcgcgtt gagctgcacg
     1981 tccatggaag tgccgagcat gttgttggtg aagtccgtct ccgaagccac gtcaaggcgc
     2041 aggccgtcgt tctcggcgct cagcgtcatg agcatcgcgc cctcgacacc ctggagtgcc
     2101 tcggtagccg tcgggtcgat aaagccggcc tgcttgtaga tctcggtcca gttcaggtag
     2161 agcgtggcga tgcgctcggc gggcacctga gcctgtagcg cggtgaatcg ggcgtcggtc
     2221 ttgaggccct tatcgtcctt gtactggtcc atgaccacct tgaggggggc cgcgtccgcg
     2281 gccagcacgg cggtagctga accgtccagc agcgtgaagt agagcgtggc gtcgcacacc
     2341 gtggccttgc ggatatccat gtcgcggtac ttctcgacca gctccgcctg gctcacgttg
     2401 tccgtaacgg tcttgaattc cgccgccggg ccttgcttgt tgagcgggtt gaagtcgagg
     2461 tccaccatgc ccacgacgtt gcgagacagg accgcgccta tctcttcgtc cccggtctgc
     2521 aatccctcga ggtccgtgct gctgggtggg agcacggcga tagtgaggtt gttgcccagg
     2581 tagctagaca tgctgccgaa gtcggtcatg ccgcccgcgc agttctcagc cgcctctcca
     2641 ccacccgcca tctcggtagt ctgctcggtg atcttggccc aggcgtcgtc gaagccgggc
     2701 tgcgacttga acgcctcgcc aagctggtcc atcgccgctt tctgggtgcc ttcgagcacc
     2761 gggtccaccg acaagaatgc aagcgcgttt ggagggagca cacggctcac ggccgcggcc
     2821 gggcgattga ccgtcctacc gaggataaag aaggcagccg cgccgcccgc cgcgagcaac
     2881 agcgccgcca cgagcaggcc aatcaccgga cccctgccgc ccttcttcgc cggggccacg
     2941 ttcacctgct gcgggggcac gtaaggggcg gtggggccag ccggaggctg ctggtactgc
     3001 tggtattgtt ggggctgttg aggagcgcca tactggggcg tgtagcccgg ctgctccgga
     3061 ggggcctggt acccgccctg gggcgtgggc gtataaggct gggtgggagc agagggtggg
     3121 taggaaggct gctccggct
//
LOCUS       DASRWZ010000176         3657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1110900, whole genome shotgun sequence.
ACCESSION   DASRWZ010000176 DASRWZ010000000
VERSION     DASRWZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110900"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3657)
                     /locus_tag="VFH60_02670"
     CDS             complement(<1..>3657)
                     /locus_tag="VFH60_02670"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET6312712.1"
                     /translation="APLSASFQTAPPPAVLRTLPSAGASEVPTDTIVTITFNRPMIPL
                     TALDSQPDPSQWVTISPNVTGRWVWLGTAAVGFHADAGFLPSTAYRVDVKAGWPDAAG
                     VTLQQGAAVSFTTVRPTILNVSPSNGSGRVPIDAPIEVSFNMPMDHASVESNISLPLP
                     SAAEWSSDSTVVTFTAGSLLEFSRSFTVQVTGSLKPARGYAAELTGGASANRWSFTTT
                     SATHVENHYPDSTGGPASPGESFGFSFNNPLAPNQDVSRFLTVDPVPQGYIGQLEVEG
                     TSVYTRGVQLLPDTTYKFALKDGLKDKWGFPVAAGTWEVKIGPLPPSVAIKGGIFQPI
                     YSEGPSRVRIEASNLAKFKLQLYSLSNDDVRSVMYSGIYDYDESTTNAPGELKREWDV
                     DVPQAPQGQAAVLYPVAALDANADRLPPGYYLLQATAPNPYSERPLQSATVLVVGRTG
                     VVTKAEGDNLFVWAADLGSGEPVPGYNLRVEQIGQSGNLQGTQHGTTGADGVVRFVLN
                     GVNTTSITSVWSDVDGDTLLATTGWSRNLYFSGYGEDGLNGERGALYTDRPIYRPAQT
                     VYFRGVLRADDDALYSLPDAARTRELSAYTYGNNGQTNVYTGTVALSPSGTFSGQFVL
                     PADAPTGSYTLTMVRIGDGRDPYTYYSVYTSFQVEEYRKPDFQVSVTVTPEVVHGDPL
                     VAGISSSYYFGGPLANVTATLNLRADLYYFGWSDPDTGESYQFGTSYPYYYYDIYPPQ
                     PNAEPVVTSLQVRTDANGLATVDVSRYVTTTAGSRSVLIEGQVQDLNNQTVANSTTSV
                     VHQGDFYVGLRTSEYVAQARQPLTVTVRTVQSLSREVQPNSAVSLRFVRLEWKAPPDG
                     TYGLPWTQEEVPVGDASVTTDANGRATYLFTPPQGGSYRIYGESRDARGNVLKSSLDF
                     YAYSDDPGYVPWRYENPQQIELVADKELYKAGDTARILVTSPFTEATGLLTVERGHLR
                     RYTIVTLQGGAPTIEVPLEEGDLPNVYIGLTLLGQERPPDGAPADWANKVTMRQGYVG
                     LTLDTSGKQLDVSIEPQGAGPFEPGTTASVKVSTRDSSGKGVPGELSLAVVDEAIYAM
                     ASDNSARLFDAFWGQRGLSVRTGTSFTSDETTVTSGYAAGETEAVPSAMPLMDEAQDG
                     AMRQAGNAGGAAPTAPQRVRVDFRDTAFWTSAVTTGADGSAVVAVPLPDNLTTWRLTA
                     QGINLATQAGTASTP"
BASE COUNT          578 a         1143 c         1282 g          654 t
ORIGIN      
        1 ggggtcgagg cggtacccgc ctgcgtcgcc aggttgatgc cctgcgcggt cagccgccag
       61 gtggtcagat tgtcgggcag gggcacggcg acgaccgcgc tgccatccgc cccggtggtc
      121 acggcagagg tccagaaggc ggtgtcgcgg aagtccacgc gcacgcgctg cggagcggtg
      181 ggtgcggccc ctccggcatt acccgcctgg cgcatggccc cgtcctgtgc ctcgtccatc
      241 agaggcatag cggagggcac agcttcagtc tcacctgccg catatccgga tgtcaccgta
      301 gtctcgtcag aggtgaagct ggtgccggtg cgcacgctca ggccgcgctg gccccagaag
      361 gcatcgaaca agcgagcgga gttgtcggat gccatggcgt agatcgcctc gtccaccacg
      421 gccagcgata gctcgccggg cacacccttg ccgctgctgt cgcgggtgct taccttcacg
      481 ctcgcggtag tacccggctc gaacggcccc gcgccctgcg gctcgatact cacgtctagc
      541 tgcttgccag aggtatcgag ggtgaggccc acgtagccct gccgcatagt caccttgttg
      601 gcccaatcgg cgggcgcgcc atcgggcggc cgctcctggc ccagaagtgt gaggccaatg
      661 tagacgttgg gcaggtcgcc ctcttccagg ggcacctcaa tcgtcggcgc gccgccctgg
      721 agcgtcacga ttgtgtagcg gcgcaggtgg ccgcgctcca ccgtgagcag gccggtagcc
      781 tcggtgaaag gcgaagtgac caggatgcgg gcggtatcgc ccgccttgta cagctccttg
      841 tcggccacaa gctctatctg ctgcgggttc tcgtagcgcc agggcacgta gccggggtcg
      901 tcgctgtaag cgtagaagtc gaggctgctc ttgagcacgt tgccacgggc gtcccggctt
      961 tcgccgtaga tgcggtagct cccgccctgc ggaggagtga acaggtaggt agcgcggccg
     1021 ttggcgtccg tcgtcacgct ggcatcgccc accggcacct cttcctgggt ccagggcagg
     1081 ccgtaggtgc cgtccggggg cgctttccac tccaggcgca cgaagcgcag gctcacggcg
     1141 ctgttgggct gcacctcgcg gctgagcgac tgtacggtac gcacggtgac ggtgaggggc
     1201 tgcctggcct gggccacgta ttcgctggtg cgcagcccca cgtagaagtc gccctggtgc
     1261 accacggagg tagtgctgtt ggctactgtc tggttgttga ggtcctgcac ctgcccttcg
     1321 atgagaacgc tgcggctgcc cgcggtggtt gtgacgtagc ggctcacgtc cacggtggct
     1381 aggccgttcg cgtcggtgcg cacctgtagg gaagtcacga caggctccgc gttcggttgg
     1441 ggcgggtaga tgtcgtaata gtagtaaggg tacgaggtac cgaactggta agactcgccc
     1501 gtgtcggggt cgctccagcc gaagtagtag aggtcggcgc gcaggttgag ggtagcggtg
     1561 acgttggcga gcggcccgcc gaagtagtag ctggacgaga tgccggccac aagggggtcg
     1621 ccgtgcacca cttcgggggt aaccgtgacg ctcacctgga agtcgggctt gcggtactcc
     1681 tcgacctgga agctcgtgta caccgagtaa taggtatatg ggtctcgccc gtcgccaatc
     1741 cgcaccatcg tcagagtgta agagcctgtg ggcgcgtcgg cgggcaggac gaactggccg
     1801 ctaaaggtgc ctgacggcga cagcgcgacc gtgccggtgt agacgttggt ctggccgttg
     1861 ttgccgtagg tgtaagcgct tagctcgcgc gtcctcgcgg cgtccggtag cgagtaaagg
     1921 gcgtcgtcgt cggcgcgtag cacgccgcgg aagtagacgg tctgcgcggg gcgatagata
     1981 gggcggtcgg tgtacaaagc gccgcgctcg ccgttcaagc catcttcccc atagccgctg
     2041 aagtagaggt tgcgactcca gcccgtggta gcgagcaggg tatcgccgtc cacgtcgctc
     2101 cacacgctgg taatggacgt cgtattgacg ccgttgagca cgaagcgcac cacaccatcc
     2161 gcccctgtgg tgccgtgctg ggtcccttgc agattccccg attgaccaat ctgctccacg
     2221 cgcagattgt agcccggcac cggctcgccg ctacccaggt cggcggccca gacgaacagg
     2281 ttgtcgccct ccgccttggt cacaacgccg gtgcgcccca ccacgagcac ggtcgcggac
     2341 tgcaagggtc gctcgctgta agggttgggc gcggtagcct gtagcaggta gtagccggga
     2401 ggcaggcggt cggcattagc gtccagtgcc gccacgggat agagcacggc ggcctggcct
     2461 tgcggggcct gcggcacgtc cacgtcccac tcgcgcttga gttcgcccgg cgcgttggtg
     2521 gtgctttcgt cgtagtcgta tataccgctg tacattacac tgcgcacgtc gtcgttgctg
     2581 agggagtaaa gctgcaactt gaacttcgcc aggttggaag cctctatgcg cacacggctc
     2641 ggcccctcgg agtagatagg ctggaaaatg ccgcccttga tagctacgct cggcggcagc
     2701 ggcccgatct ttacctccca ggtgcctgcc gccaccggga agccccactt gtccttgagg
     2761 ccgtctttca gcgcgaactt gtaggtcgta tcgggcaaca gttgcacgcc ccgcgtgtag
     2821 acgctcgtac cctccacctc aagctggccg atgtagccct gcggcacggg gtccacggtg
     2881 aggaaccggc taacgtcctg gttgggcgcg agggggttgt tgaacgagaa gccgaacgac
     2941 tcgcctggag aggcgggacc gcccgtgcta tcgggataat ggttctccac gtgcgtggca
     3001 ctggtcgtgg tgaagctcca gcggttggcc gaagccccgc ccgtaagctc ggcggcgtag
     3061 ccccgcgcgg gcttgaggct gccggtgact tgcacggtga agctgcggct gaactccagg
     3121 agactgcccg ccgtgaacgt caccacggtg ctgtcggacg accattcggc ggcgctcggt
     3181 agcggcaggc tgatgttgct ctccaccgag gcatggtcca tcggcatgtt gaagctcacc
     3241 tcgatgggcg catctatggg cacgcggccc gacccattgg agggcgacac gttgaggatg
     3301 gtggggcgca ccgtggtaaa gctcaccgca gcgccctgct gcaacgtcac accggcggca
     3361 tcgggccacc cggctttcac gtccaccctg taggcggtcg aagggaggaa gcccgcatcg
     3421 gcgtggaagc ccaccgccgc cgtaccaagc cacacccagc gtcccgtcac gttgggcgaa
     3481 atcgtcaccc actgcgaggg gtcgggctgg ctgtcgagcg cggtgagggg gatcatcggg
     3541 cggttgaaag tgatagtgac aatcgtgtcg gtgggcacct cgctggcccc cgcgctgggc
     3601 agggtgcgca gcacggcggg agggggtgcc gtctggaagc tggcggacag gggagcg
//
LOCUS       DASRWZ010000177         1870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_699598, whole genome shotgun sequence.
ACCESSION   DASRWZ010000177 DASRWZ010000000
VERSION     DASRWZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699598"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            670..1833
                     /locus_tag="VFH60_02675"
     CDS             670..1833
                     /locus_tag="VFH60_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6312713.1"
                     /translation="MRSTGPLSATRHHEHHSLIELPPIDVDTGEVDIPAEERAQIEAE
                     SPIMAESVEDISEDRSWLSHKLLNVRTLFSFALAIGLIVFLFTRLDIDVGATWSIILT
                     ADPLILLTGFLVYYSAFWLRATRWKVLLQNAGHGKQDSPKQLPSTNGLVEIIYLSWFV
                     NCIVPAKLGDAYRSYLLKRNSGISFSSTIGTIFAERVVDLLVLFGLLGASFALVGQRL
                     ASEGEVNLGLVMLFGAGMVLALIAGLIALRFFGHVVLRFVPQRFKTKFTQFRQGILHS
                     FKRRSLPTVLGLTVVIWFLEAARLFFVTRSLDAHVVTISAVIFIALLSSLLTTLPLTP
                     GGAGVVEGAVTVALGLFLKDHNMAVSIAILDRLINYWSLVVGGLILYLISKRR"
BASE COUNT          315 a          623 c          532 g          400 t
ORIGIN      
        1 gtaagagttc cgccgggcgg gtgtatatgg ccgactggct gccggaagtg gtcatgaggt
       61 gctcctttgg cgttttgctg gattataaac gaggtttgcg ggcgtgcaaa ggaaggagac
      121 gaaaccgcca agacgcaaag acgccaagga ccacgccaag ttaaggattg tgttcttagc
      181 aaacaggacc agggtacatg ctgtcgtatc ctggtccttt tgcactatct aacccttacc
      241 actctttgcg cgttctttgc gttttttgcg tctttgcgtt ttcgtactct tggcatggtc
      301 cttggcgtct tggcggtttt gtctctttac atgttgctat tgccccgggc gttgtgcatt
      361 ggtatgttac ctgctgttgc agagggtacg cgcctaacgc agcgtaacgt gtttatggta
      421 atattccaga gactctgttg agccgggtac cgcccccggt actcactctc tatcggaggc
      481 acagcaattc tgaactctcg acccggcccc ggaacgcctg ccggacagga ccgccccgcg
      541 cccaacaacg gctcggacgg cctcgacggg catgatggtg acggcagcgt cgctgacggc
      601 gtcgcccgtg acggcaacac cccggacggc tcgaacggcc ccacggagcc gcggcccact
      661 cgcgcgggga tgaggagcac cgggccgctc tcggctaccc ggcatcacga acaccactcg
      721 ctgatagagc ttccacccat cgacgtggac accggcgagg tggacatccc cgccgaagag
      781 cgcgctcaaa tcgaggcgga atcccccatc atggccgagt cggtggagga tatctccgag
      841 gaccgctcct ggctcagcca caagctgctc aacgtccgca ccctcttctc gttcgccctg
      901 gcaatcggcc tcatcgtctt cctctttacg cgcctggata tcgacgtcgg ggccacgtgg
      961 agcatcattc tcaccgccga cccccttatc ctgctgacgg gcttcctggt ctactattcc
     1021 gccttctggc tgcgcgccac ccgctggaag gtgctcctgc aaaacgcggg gcacggcaag
     1081 caggactcgc ccaagcaact gcccagcacc aacggcctgg tggagattat ctaccttagc
     1141 tggttcgtca actgcatcgt gcccgccaag ctgggcgacg cctaccggag ctacctgcta
     1201 aagcgcaact ccggcatttc gttctccagc accataggca ccatctttgc cgagcgcgtg
     1261 gtggacctgc tggtgctgtt cgggctgctt ggcgcgtcct tcgcgctggt ggggcagcgg
     1321 ctagccagcg agggcgaggt caacctgggt ctcgtgatgc tgttcggggc gggcatggtg
     1381 ctggccctca tcgcggggtt gatcgccctg cgcttcttcg gtcacgtcgt attacgcttc
     1441 gtaccccagc gcttcaagac caagttcacc cagttccgcc agggcatcct gcactcgttc
     1501 aagcgccgca gcctgcccac cgtgctcgga ctcaccgtgg tcatctggtt cctggaagcg
     1561 gcccgcctct tcttcgtcac gcgctccctc gacgcccacg tggtcaccat ctctgccgtc
     1621 attttcatcg ccctgctaag ctctctgctc acgaccctgc ccctcacccc aggcggcgcg
     1681 ggcgtagtcg agggtgccgt caccgtcgcc ctgggcctct tcctcaaaga ccacaacatg
     1741 gccgtctcca tcgccatcct agaccgcctc atcaactact ggagcctggt agtaggcggc
     1801 ctcatcctct acctcatcag caaacgcagg tagtgccgag tgccgagtgc cgagtgccga
     1861 gtgccgagtg
//
LOCUS       DASRWZ010000178         1805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1042421, whole genome shotgun sequence.
ACCESSION   DASRWZ010000178 DASRWZ010000000
VERSION     DASRWZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042421"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..879
                     /locus_tag="VFH60_02680"
     CDS             196..879
                     /locus_tag="VFH60_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312714.1"
                     /translation="MQQHRPGPNAYAGVTTEQDRHTQRVYRKRVRGVRVRWLISGLLL
                     GLLGGVLLTIVVSALVVTKRPLPIKEDLSEPDVSVTVSESYLNRATAERVATFSTGME
                     YLTLTSLRLDLQPGHRMDLQPTFHSDLGFFVFDTSAIVSNQLAVENGKLVIRMVGDPQ
                     LGDLNVSLDALPLDLKGTLASAVDRINNDLLISEINESLISGFGGTDFTIYGLSTEGD
                     RLTIRLRER"
     gene            complement(945..>1805)
                     /gene="glp"
                     /locus_tag="VFH60_02685"
     CDS             complement(945..>1805)
                     /gene="glp"
                     /locus_tag="VFH60_02685"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET6312715.1"
                     /translation="FRSVKMGDSVRRAGEDMRQGDLVLGPGTIIRPAEIGLLASVGKA
                     LVAAHRRPRVVVLATGDELVEVDQTPGPGQIRNTNNYAIAAQLQSWGAVPLNLGVARD
                     SREHLETKLHEALDLEPDLIISSAGVSVGDHDFVKDVLLSMGEVHMWRVRVRPGKPLL
                     FGRLGEREVPLLGLPGNPVSSMVTMELFARPAILKMLSKSRLHRPTVTARVTEAMDYG
                     KGREHYVRGIVSKEGDEYVCRPTGGQGSNLLTSMSRANALLIIPESVSRVEPGDQLKA
                     LMLDWPEDVF"
BASE COUNT          347 a          585 c          544 g          329 t
ORIGIN      
        1 gaggtggccc ggctgatagg caccagcaaa gaggcggtca tacacgctat cacgcagggc
       61 gagctaaagg ccgaccgcaa aggccgcgac gtggtctgca tccagcatca cgacgtagta
      121 gagtggcttc gccgccgcaa cgcgacccgc tagcgcacag cacgcccggc attcacaaga
      181 cggcccctac acactatgca acaacaccgc cccggaccta acgcttacgc gggggtcaca
      241 accgagcagg atcgccacac ccagcgggta taccgcaagc gggtgcgcgg cgtgcgggtg
      301 cgctggctca taagcgggct gctgctgggc ctcctggggg gcgtattgct caccatcgtg
      361 gtgtccgcgc tggtggtcac caagcggccg ctgcctataa aggaggacct gagcgagccg
      421 gacgtgtcgg tgacggtcag cgaaagctac ctcaacaggg caaccgccga gcgcgtagcg
      481 actttcagca ccggcatgga gtacctgacc ctcaccagcc tgcgcctcga tttgcagccc
      541 ggccaccgca tggacctcca gccgaccttc cactctgacc tggggttttt cgtcttcgac
      601 accagcgcca tcgtgagcaa ccagctcgcg gtggaaaacg gcaagctggt gataaggatg
      661 gtgggcgacc cacagctagg cgacctcaac gtgtccctcg acgccctgcc tctcgacctc
      721 aaaggcacct tagcctccgc cgtggaccgc atcaacaacg acctgctcat ctccgagatc
      781 aacgaatcgc tcatctcagg cttcggcggc accgatttca ccatctacgg cctcagcacc
      841 gaaggcgacc gcctgacgat acggctgagg gagaggtagg attttagatt ttagattttg
      901 gattttagat ttagaagaac tcctaataat ccaaaatcta aaatctaaaa tacgtcttcg
      961 ggccagtcga gcatcagcgc ctttagttgg tcgccgggtt cgacgcggct gacggattcc
     1021 gggatgatta gcagggcgtt ggcgcgggac atggaggtga gcaggttgga accctggccc
     1081 ccggtggggc ggcagacgta ctcgtcccct tctttggaga cgatgccgcg cacgtaatgt
     1141 tcccggccct tgccatagtc catcgcttct gtgacgcggg cggtgacggt ggggcggtgc
     1201 aggcgcgact tgctgagcat cttcaggatg gccggcctgg cgaatagctc catcgtcacc
     1261 atgctcgata ctgggttgcc gggcaggcct agcagaggca cctcgcgctc gcccaatctg
     1321 ccgaacagca gcggcttgcc aggcctcacg cgcacccgcc acatgtgcac ctcccccatc
     1381 gaaagtaaca cgtccttcac aaagtcgtgg tcgcccaccg atacacctgc tgaggatatt
     1441 atcaggtccg gctccaggtc tagcgcttcg tgtagcttgg tttcaaggtg ctctcggctg
     1501 tcgcgggcca cgcccaggtt gaggggcacc gcgccccagg actgtagctg cgcggcgatg
     1561 gcgtagttgt tggtattgcg tatctggccg gggccggggg tctggtccac ctccacaagc
     1621 tcgtcacccg tagccagcac caccactcgc ggcctgcggt gcgcagccac gagtgccttg
     1681 cccaccgatg ccagcaggcc gatttcagcg gggcgaataa tggtgccggg accgagcacc
     1741 aggtcgcctt gtctcatgtc ctcgcccgcc ctgcgcacgc tgtcgcccat cttgacgctg
     1801 cggaa
//
LOCUS       DASRWZ010000179         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_768518, whole genome shotgun sequence.
ACCESSION   DASRWZ010000179 DASRWZ010000000
VERSION     DASRWZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768518"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1927
                     /locus_tag="VFH60_02690"
     CDS             164..1927
                     /locus_tag="VFH60_02690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HET6312716.1"
                     /translation="MSVVYPSIDSPEFEQGFSALVGSIEELARLFDERKIEKGESKPL
                     DDETVGAFEAVVNRLNEVLKDTMTLGAYIRGFTTTNSRDNLAQAKLSELQNSTLILSK
                     LSTRLTAWIGSLDVEELVNRSQVAAGHAYMLRKAQEQSRHLMSPVEEELAAELNLTGG
                     TAWSKLHNNVTSQLAVQVDLKGETRTLPMPSVRNLGFDEDREVRRKGYEAELEAWKTV
                     EVPLAQALNSIKGEVNTLTRRRGWGSALDEAVFNANIDRQTLDAMMQAAHESFPDFRR
                     YLKAKARALGLEKLAWYDVTAPLSKGNRVWEDQEAAGFIVEQFGTYSEKMSRFARRAF
                     EEDWIDAEPRSGKRDGAFCMWLRGDESRILSNYKPAYGGMSTLAHELGHGYHNMVKAH
                     RTMLQKTTPMTLAETASIFCETIVRNAALQHGSPDEQLSILEASLESSTQVVVDITSR
                     FLFESRVFEMRKQRELSADEFCDLMLQAQRETYGDGLDENYLHPYMWAVKGHYYSTGL
                     SFYNFPYMFGLLFGLGLYAQFQQDADTFRTGYDDLLSSTGLDDAATLASRFGIDIRTP
                     DFWRSSLAIIRADIDRFESLV"
BASE COUNT          432 a          642 c          623 g          333 t
ORIGIN      
        1 cgtaaaggag caatagcaac agagcggtga atttcgatat atggtgactg agccgccgtt
       61 acgtgctacg cgttacgtgt tactttcccc tcctcccaaa actaacactt agagaggaca
      121 cattatggtc gcaactgcag agactaagtt accgcattgg aacatgtcgg tggtatatcc
      181 cagcatcgac tcgcctgagt tcgagcaggg gttcagcgcg cttgtcggca gcatagagga
      241 attggcccgg ctgttcgatg agcgcaagat tgagaagggc gagtccaagc cgctggacga
      301 cgagacggtc ggcgcgttcg aggcggtggt caaccggctg aacgaggtgc tgaaggacac
      361 tatgacgctt ggcgcgtaca tacgtggctt caccaccacc aactcccgcg acaaccttgc
      421 ccaggccaag ctgagcgagt tgcagaactc caccctcatc ctgagcaagc tcagcacccg
      481 cctgaccgcc tggataggct cgctcgacgt ggaagagctg gtaaatcgct cccaggtggc
      541 cgccggccac gcgtacatgc tgcgcaaagc ccaggagcaa tcgcgacacc tgatgtcgcc
      601 cgtcgaggaa gagctggcgg cggagctgaa cctcaccggc ggcacggcct ggagcaagct
      661 gcacaacaac gtcacctcgc agcttgccgt gcaggtggac ctcaagggcg agacgcgcac
      721 gctgccgatg ccctcagtgc gcaacctggg cttcgatgag gaccgggagg tccgccgcaa
      781 gggctatgag gccgagctag aggcctggaa gacggtcgag gtgccgctgg cccaggccct
      841 caacagcatc aagggcgagg tgaacacgct caccaggcgg cgcgggtggg ggtcggccct
      901 cgacgaggcg gtcttcaacg ccaacatcga ccggcagacc ctcgatgcta tgatgcaggc
      961 cgcccacgag tcgttcccgg acttccgccg ctacttgaag gccaaggcac gagcgttggg
     1021 gctggagaag ctggcctggt acgacgtgac ggccccgttg agcaaaggca atcgcgtgtg
     1081 ggaagaccag gaagccgccg gcttcatcgt ggagcagttc ggcacatact cggagaagat
     1141 gagccggttc gcccggcgcg ccttcgagga agactggatt gacgccgagc cgaggtcggg
     1201 caagcgcgac ggggccttct gcatgtggct gcggggcgac gagtcgcgca tcctatccaa
     1261 ctacaagccc gcttacgggg gcatgagcac gctggcccac gagcttgggc acggctacca
     1321 caatatggtg aaggcccacc gcaccatgtt gcagaagact acccccatga ccctggccga
     1381 gaccgccagc atcttctgcg agacgatcgt gcgcaacgcc gccctacagc acggcagccc
     1441 cgacgagcag ttgtccatac tggaagcctc gctggagtcg agcacgcagg tggtggtgga
     1501 catcaccagc cgcttcctgt tcgagagccg cgtgttcgag atgcgcaagc agcgcgagct
     1561 ttcggcggac gagttctgcg acctgatgct gcaagcccag cgggagacct acggcgacgg
     1621 cctcgacgag aactacctgc acccctatat gtgggcggtg aaagggcact actacagtac
     1681 cggcctgtcg ttctacaact tcccctacat gttcggcctg ttgttcggcc tgggcctcta
     1741 cgcccagttc cagcaggacg ccgacacctt ccgcaccggc tacgacgacc tgctctcctc
     1801 caccggcctc gacgacgccg ccaccctggc ctcccgcttc ggcatcgaca tccgcacccc
     1861 cgacttctgg cgctccagcc tcgccatcat ccgcgccgac atcgaccgct tcgagtccct
     1921 cgtgtagcag caggagacga aaacgcaaag acgcaaagac gcaaagaacg cgcagagatt
     1981 aagattggtg ttctaagcaa aagggccagg atacatgatg tagtatcctg
//
LOCUS       DASRWZ010000180        10350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1316809, whole genome shotgun sequence.
ACCESSION   DASRWZ010000180 DASRWZ010000000
VERSION     DASRWZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10350
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316809"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1674)
                     /locus_tag="VFH60_02695"
     CDS             complement(70..1674)
                     /locus_tag="VFH60_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6312717.1"
                     /translation="MSRLWKRFIRASAFVSKEFNEVRRQPRLVLSLVLGPFLILFLFG
                     IGYQGETNNLRALVVVPPTGQFSNDVAEYRAMTGNQLNVTGVTNSLPEALDRLKERSV
                     DIVVQVPEDAARQISAGSQARVPVYFNEVDPLRRDWITYLSFLYINEINKNTVAAVAS
                     QGQEQAGDLRSVLARMRNSLAATEQSVRNGQTQEASQEAQRMQGSSTNVQLTVVLLAQ
                     LLASENALVKPPSAQDPDQVNLANGGEAANDLSEDLRALNVALNDPNTDRATLLQRID
                     RVRTDLDEMDRLTQQFQSINPLVLAAPFYPQVENTAPIKANFTSFYAPGVLVLILQHI
                     AITLTALSMVRERLLGTVELFRVSPVSPMEILTGKYTGYLLLLGGIASILLVMMSNNI
                     VIEGFPLSLGVPILGDWVLLFFSIVLVIFSSVGLGLLISTISKSESQAVQISMLVLLA
                     SVFFSGFFLRIEALWWPVRAVSYALPVTYGVSSLQVIMLRGGVPSPAAWLGDVPFPAV
                     LLALFTLGLFFSLIGYLLFRRSLRQQ"
     gene            complement(1667..2749)
                     /locus_tag="VFH60_02700"
     CDS             complement(1667..2749)
                     /locus_tag="VFH60_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6312718.1"
                     /translation="MSQSMVNHTLPIEVQDPARRTLELPVVNQPPAPPMIDVRNVSKV
                     LGGNTAVADLNFQVQQGEIFGFIGPSGSGKTTTIRLLTGVYTPTSGELTVMGVEPSHP
                     GRRVQEHFGYMPQLFVLYPNLTVQENLSFVAQLYGMGRVARNRRVKELLQFVELYDDR
                     NKLATNVSGGMQRRIELAAALLHNPALLFADEPTAGIDPVLRGKFWEEFRRLRDEGRT
                     LFITTQYVGESEYCDRVGVIREGRLVAVDTPTALRRRAFGGDMVDVVVNGLSDAIVRS
                     LEELPIVKYVQRVSANELRASVGEAGPAIPPMLDALNSLSCEVKRIEEYRPNFDDVFI
                     RLMEQDEEATAQAEAKGTSTTETAHV"
     gene            complement(2756..3379)
                     /locus_tag="VFH60_02705"
     CDS             complement(2756..3379)
                     /locus_tag="VFH60_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312719.1"
                     /translation="MRFDLDTEIRYPTGERAGVLRHVIVNEANEVVEVAMATDTLVSR
                     FVRVPVGALSTEPGDVLTINLTQDEIDELPAYAEERLPAVPEGWQFPDDAAPGADAFP
                     ATMLQPIIPIVEVADIPEGELSIGQGTELACLDGRWGIVDEVLTGDDGQVYALVGRPD
                     AVEEADRVIPVQLVQHADANQVVLNCTLEDIANYTQELQGELEEPDR"
     gene            complement(3383..4636)
                     /locus_tag="VFH60_02710"
     CDS             complement(3383..4636)
                     /locus_tag="VFH60_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HET6312720.1"
                     /translation="MQTSHDSTQAPATLIDTLWQEAERLREAMIDFTQRLIQTISLPG
                     QEGAIAQVVIAEMESLGYDHVSIDEAGNVIGLIRATAAEEGATPPSIMFNTHMDHVDV
                     GDPANWPFPPYEGVVKNGEIWGRGASDLKGSLACHVYTGALLKRAGLPLANDVYVVGV
                     VQEEVGGAGSAVLAENLHTDYVVIGEPSYNKIALGHRGRIEIVVTITGKSVHASVPDT
                     GVNPLYSMSRFLQAVQTLTFEADPANPDLGPTSIAPTLITTDQTSKNVVPGQCTVILD
                     VRNSPVDNEEQVLQRVRALLESSLQDGATGSAEVPDLVLTTYTGVTQVLHGHGPFGVA
                     ADSPLTREVLSIVGGALRREVPTQMWKFATDAGYFVNKGAKVIGFGPGYEHVIHTVDE
                     RISIDMMVEGMVACAALALQMRGEG"
     gene            4903..5343
                     /gene="mscL"
                     /locus_tag="VFH60_02715"
     CDS             4903..5343
                     /gene="mscL"
                     /locus_tag="VFH60_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET6312721.1"
                     /translation="MFSEFKKFINRGNVIELAVAFVIATAFAAVVKSLVDNIIMPPVG
                     ALLGGFDFTNLYINLSNRPYPSYAEATAAGAPVIGYGIFINTVITFLIVTFVVFLLVR
                     AYNKGVKQPAPEVDTKDCPYCMTAIALKATRCPACTSDLAVAMG"
     gene            5588..6181
                     /locus_tag="VFH60_02720"
     CDS             5588..6181
                     /locus_tag="VFH60_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6312722.1"
                     /translation="MAVAQTKVIIADDDPIIRMDLREMLSGMGYNVIGEAADGKHAVD
                     LARNLQPDLMIMDIRMPELDGIEAARTLTQESIAPVLLLTAYSEPDLVQRAAQAGVIG
                     YLVKPFREAQLAPAIEVTLARFREFEALGKELGDIKSAMEARKVVERAKGLLMDRFGL
                     SESEAFRRIQKRSMDSRKPMRDIAEAILLASEMEKDS"
     gene            complement(6305..6697)
                     /locus_tag="VFH60_02725"
     CDS             complement(6305..6697)
                     /locus_tag="VFH60_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312723.1"
                     /translation="MKQVPENRNNTSNQKSLTVYAVLQTLTLFLTWGWFAALWAVAMV
                     AFTMSDLEWWLLVASACAGLYGLRPNLERAIDTAWLKGITDSKALGERFAQMKPIKRR
                     VAVIFSVVTLALLTILLVIFGFNLVGTA"
     gene            complement(6852..7514)
                     /locus_tag="VFH60_02730"
     CDS             complement(6852..7514)
                     /locus_tag="VFH60_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HET6312724.1"
                     /translation="MSILTRIATLINANINAMIDAAEDPEKMVNEYLRQMTEQLSEAR
                     AATAMAMADETRVRSQYERFKSEADDWQRKAEMAVQADQDDLAREALTRRASAQKLAD
                     TYQAQWESQHDQVGDLREALAKLEAKISEAETKREIIIAKQRRAATQEAITNALQNVQ
                     GQTADSSLQRMENEVDQRLARASAMAELSGQDLDSRFSDLETENQVNTDLADLKRKFN
                     KG"
     gene            complement(7700..8434)
                     /locus_tag="VFH60_02735"
     CDS             complement(7700..8434)
                     /locus_tag="VFH60_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HET6312725.1"
                     /translation="MKTLLAKRIPLAWSLLALIVLAAAGILWLLATRASAPANAVLIS
                     SAPTHTPTAEAGLPGATTQAGGVASTSAPTQPTPQALVVYVSGEVVSPGVYTLPLGSR
                     LADAVAASGGFTDSANREGINLAARIADEQHISVPREGEPVQAAEDETKGQGGAPGAS
                     VTGDEAPPSPQPAVTGAPPPRVNVNTANAQALEELPGIGEVLAARIINDRQANGPFRT
                     VDDLARVPGIKEHVLSQLRGLVTVGP"
     gene            complement(8481..>10350)
                     /locus_tag="VFH60_02740"
     CDS             complement(8481..>10350)
                     /locus_tag="VFH60_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET6312726.1"
                     /translation="PAAYASAALEGRYYEQTGLTLAPEFLDYYDAQGGVLLFGYPVTP
                     ARLENGYLVQWTERQRLEHHPEHAGTPHEVLLGLLGRELTAGYSGPTFRAQLHAAAHT
                     SQADQDATSTTGHFFPETGQTVAEPFLSYWRDNGGLRVFGYPISRLFADEQGLQQQWF
                     ERARFEYHPELAPDHEVLLGHLGLEALKNAEAPRYELQVDYAPAPEGKLEIGIAQGGE
                     SDDAGFFDNVRELGRDLGPGLVRLDNIFNYYGIVGRAPDGKLTYRWDDFDRVLDGIKA
                     MGKEPFICLSYMPETMSVSGSSRVVPPARYEEWAELVAATVRHVNGERRLGVRYWEVW
                     NEPNLWDFWQGSYPEYLRLYDVTVEAALAADPNIRIGGPAVASYQPDHINEFMQHEAS
                     LGVKGRIDFVSWHSYGRQPEELAANIRAVREIAGKYPQFSPELIITEFNVLQGGPLDT
                     SANGATDTADGAIAFLSSIESMQRERLDKALLFELKDGIGPRSYWGRWGMLTYDGLAK
                     PVYHAYKAYQERPGGQLPVRSGNAPTDGTLGLLAYGHPANATLFLWYTGLAPARVKVT
                     LPDTFADAEFDLTLFDRQNNNPARTGDSTLRTLSPRNAGDIVLDLQPRSLVILRSR"
BASE COUNT         1766 a         3087 c         3473 g         2024 t
ORIGIN      
        1 actccccaag ccctaatagc ttggcgcgct cttggcgtcc ttggcgtctt ggcggttccg
       61 tttctttgtt tactgctggc ggaggctccg gcggaacagg aggtagccga tgaggctgaa
      121 gaacaggccg agggtgaaga gggccaggag cacggcgggg aagggcacgt cgcccagcca
      181 ggcggcgggg cttggcaccc ctcctctgag catgattacc tgcaagcttg agacgccgta
      241 ggtgacgggc agggcgtagg agaccgcgcg tacgggccac cagagggctt cgatgcggag
      301 gaagaaaccg ctgaagaaca cggacgccag cagcacgagc atcgagattt gcaccgcctg
      361 gctctcggac ttcgagattg tggaaatcag caggccgagg ccaaccgagg agaagatcac
      421 cagcacgatt gagaagaaga gcagcaccca gtcgcccagg atgggcaccc cgagactgag
      481 ggggaagccc tcgattacga tgttgttgct catcatcacg agcaggattg aggcgatacc
      541 gcccagcagc agcagatacc cggtgtactt gcccgtaagt atctccatcg ggctgaccgg
      601 cgacacgcgg aaaagctcca ccgtgccgag caggcgctcg cgcaccatag agagagcggt
      661 aagcgtaatc gcgatatgct gcaagatcag caccagcacg ccgggcgcgt agaacgaggt
      721 gaagttagcc ttgatgggcg cggtgttctc gacctgtggg tagaaggggg ccgccagcac
      781 cagggggttg atagactgga actgctgcgt gaggcggtcc atctcatcga ggtcggtgcg
      841 cacgcggtcg atacgctgca agagcgttgc gcggtcggta ttcgggtcgt ttagcgccac
      901 gttcagggcg cggaggtctt cggagaggtc gttcgccgct tcgccgccat tggcaaggtt
      961 cacctggtcg gggtcctgcg ccgatggggg cttgacgagg gcgttttcgg aggcgagcag
     1021 ctgggcaagc aagaccacgg tcagctgcac gttggtgctg ctgccctgca tgcgctgggc
     1081 ctcctggctc gcctcctgcg tctggccgtt gcgcacgctc tgctcggtgg cggcgaggga
     1141 gttgcgcatg cgagcgagta cggagcgcag gtcgcccgcc tgttcctggc cctggctggc
     1201 gacggctgcc accgtgttct tgttgatctc gttgatgtag aggaacgaaa ggtaggtaat
     1261 ccagtcgcgg cgaagggggt ccacctcgtt gaagtagacg ggcacgcgcg cctgcgagcc
     1321 tgcggaaatc tggcgggcgg cgtcctccgg cacctgtacc acgatatcca cggagcgttc
     1381 tttgaggcgg tccagggcct cagggaggct gttggtgact ccggtgacgt ttagctggtt
     1441 gcccgtcatg gcgcggtact cggcaacgtc gttggagaac tggccggtgg gcgggacgac
     1501 cacgagggcg cgcaggttgt tggtctcgcc ctggtagcca atgccgaaca ggaacagaat
     1561 caggaacggc ccgagcacga gggagagcac cagcctgggc tggcggcgga cctcgttgaa
     1621 ctctttgctg acaaaggcgc tggcgcgtat gaagcgcttc cacaacctag acatgggccg
     1681 tctcggtcgt gctggtgccc ttggcttctg cctgggctgt ggcctcttcg tcctgctcca
     1741 ttagcctgat gaacacgtca tcgaagttgg gccggtactc ctcgatgcgc ttgacctcgc
     1801 aactgaggga gttgagcgcg tccagcatgg ggggtatggc aggaccggct tcccctacgc
     1861 tggcgcgcaa ctcgttggcc gatacgcgct gcacgtactt cactatgggc aactcttcca
     1921 gggagcgtac gatggcgtcg gacaggccgt tcaccacgac gtccaccata tcgcccccga
     1981 aggcgcgacg acgcagggcc gtaggcgtgt ccacggccac gagtcgcccc tcgcgtatga
     2041 cgcccacgcg gtcgcagtac tcgctctcgc ccacgtactg agtcgttatg aatagggtgc
     2101 ggccttcgtc gcggagcctg cggaactcct cccagaactt gcccctgagc accgggtcga
     2161 tgcccgcggt aggctcgtcg gcgaatagga gggccgggtt gtgcagcagg gcggccgcaa
     2221 gctcgatgcg ccgctgcatg ccgcctgaga cgttggtggc gagcttgttc ctgtcgtcgt
     2281 atagctccac gaactgaagt agctccttga ccctgcgatt gcgcgccact ctccccatac
     2341 cgtaaagctg ggccacgaag gaaaggttct cctgcacggt gaggttgggg tagagcacga
     2401 acaactgggg catgtagccg aaatgctcct gcactcgcct gccggggtgg ctaggctcca
     2461 cccccatgac ggtgagctcg cccgatgtgg gcgtgtagac gccggtcagc aggcggatgg
     2521 tggtggtctt accggagccg ctggggccga tgaagccgaa tatctcccct tgctgcacct
     2581 ggaagttgag gtcggctacg gcggtgttgc cgcccaggac cttgctgacg ttgcgcacgt
     2641 cgatcatggg cggggcgggt ggctggttga ccacgggcag ctcaagggtg cgcctggccg
     2701 ggtcttgcac ttcgatggga agggtgtggt tcaccatgct ttgggacatc tgctgctacc
     2761 tatccggctc ttccaactca ccctgcaact cctgggtgta gttggctata tcttcaagcg
     2821 tgcagttgag cacgacctgg ttggcgtcgg catgctgcac gagttgcaca ggtataaccc
     2881 ggtcggcctc ttcgacagcg tcgggtcgac cgacgagagc gtacacctgg ccgtcgtcgc
     2941 cggtgagcac ctcgtccacg atgccccacc gtccgtccag gcaggcaagc tcggtgccct
     3001 ggccgatgga gagttcgcct tcgggtatgt cggccacctc aacgatgggg atgatcggtt
     3061 gcagcatcgt ggccgggaag gcgtccgcgc caggcgctgc atcgtcgggg aactgccagc
     3121 cttcggggac ggcaggcagg cgttcctcgg cgtaggccgg tagctcatct atctcgtcct
     3181 gggtgagatt tatggtcagc acgtcgcccg gctcggtgct caaggctccg acgggcacgc
     3241 gcacgaaacg cgagacgagg gtgtcggtag ccattgctac ctctaccacc tcgttagctt
     3301 cgttgacgat gacgtgccgc aacacccccg cgcgctcgcc agttgggtag cggatctcgg
     3361 tgtccagatc aaacctcatg gattacccct ctcctctcat ttgcagggcc agggcggcgc
     3421 aggccaccat gccctctacc atcatgtcga tggatatccg ctcatcgacg gtgtgaatta
     3481 cgtgctcgta gccaggcccg aagccgatca ccttagcgcc tttgttcacg aagtagcccg
     3541 cgtcggtggc gaacttccac atctgcgtgg gcacctctcg ccgcaacgcc ccgccgacta
     3601 tgctcagcac ttctcgcgtg agggggctgt cggcagcgac cccaaatggc ccgtgcccgt
     3661 gcaatacctg cgtcacgccc gtgtaggtgg tgagcaccag gtcgggcact tcggcgctgc
     3721 cggtggcccc atcttgaagc gacgactcca ggagcgcccg cacgcgttgg agcacctgct
     3781 cctcgttatc gaccgggctg ttgcgcacgt ccagtatgac cgtgcactgg cccggaacga
     3841 cgttcttgct ggtctggtcc gtggtgatca gggtcggcgc aatggaggtc ggccccaggt
     3901 cggggttggc ggggtccgcc tcgaacgtca gtgtctggac cgcctgcaaa aacctcgaca
     3961 tagagtacaa cggattcacg cccgtatccg gtacgctcgc atgcaccgat ttgccggtaa
     4021 tggtcacgac gatctcgata cggcccctgt ggcccagcgc gatcttgttg tacgacggct
     4081 cgccgattac cacgtagtcg gtatgcaagt tctcggccag caccgcgcta cccgcgcccc
     4141 cgacctcctc ctgcacgacg cccaccacgt acacgtcgtt ggcaagaggc aggccggcgc
     4201 gcttgagcag ggcacccgtg tagacgtggc aggcgagaga gcctttcaga tcgctcgcgc
     4261 cccggcccca gatttcgccg ttcttgacga cgccttcgta gggcgggaac ggccagttgg
     4321 cggggtcgcc cacgtccacg tggtccatgt gggtgttgaa catgatgctc ggaggggtcg
     4381 cgccttcctc ggccgctgta gcccgtatca ggccgatgac gttcccggcc tcatcaatgc
     4441 taacgtggtc gtagccgagg gactccatct ccgcgatcac gacctgggcg atggccccct
     4501 cctggccggg caggctgatc gtctgtatca ggcgttgcgt gaagtcaatc atcgcctcgc
     4561 gcaggcgttc ggcctcctgc cagagggtgt ctatcagtgt ggcgggggct tgagtgctgt
     4621 cgtgggatgt ttgcatgcgt atgtacctcc tggtgtcgtc gtgctagtcc caagtgtagc
     4681 gcaaaacgtc ggtggcgggt aggacagggg ctgcggtggc tggtgccttg taagcgttca
     4741 gaaggggatg gacgggggta ggggctgccg ccaaaccgtt ctaccgcaac tgtgaacacc
     4801 tactgcgtcc atatggtttg acagtgcggt ggcgtggtgc taccatgcgc atgctctgaa
     4861 ggtcttgtcg taaggcacat atttacctgg gaggggggaa ccatgttcag cgagttcaag
     4921 aagttcatca accgggggaa cgttatcgag ctggcggtgg cctttgtaat cgccactgcg
     4981 tttgcggcgg tcgtcaagtc actggtggac aacataatca tgccgccggt aggggcgctg
     5041 ttgggcggct ttgatttcac gaacctttac atcaacctga gcaacaggcc gtacccatcg
     5101 tacgcggagg cgacggcagc gggagcgcct gtgatagggt acggcatctt catcaacaca
     5161 gtcattacgt ttctaattgt caccttcgtc gtattcctcc tcgtgcgagc ctacaacaaa
     5221 ggggtcaagc agccagcgcc tgaagtggac acgaaggact gcccgtactg catgacggct
     5281 atagctctga aggccacacg ctgcccggct tgcacgtccg atctggcggt agccatgggc
     5341 tgatttaggg cgcgtcacgc gtcagactga gggccggcct gctttcacca ctcgttgtgc
     5401 ttgcccgcaa aaggggcagt ttggttgctc catttatccc cctaatcttg cctcttctcg
     5461 tcacttttta tcacgtccta tcccacctat gattgacggg tgcagataac gcccctataa
     5521 tgaattgcct caggctgccg gaacgtggcg gagggtgtta gagcgacctt tatagtgagg
     5581 gaacccagtg gcagttgcac aaaccaaggt aataatcgcc gacgacgacc ccataatcag
     5641 gatggacctg cgggagatgc tgtcgggcat gggctacaac gtgatcgggg aggccgcgga
     5701 cggcaagcac gcggtcgatt tggcccgcaa cctgcagccc gacttgatga taatggacat
     5761 ccgcatgccg gagcttgacg gtatcgaggc ggcccgcacg ctcacgcagg agagcatcgc
     5821 ccccgtgctg ctgctgaccg cgtacagcga acctgacctg gtgcagcggg cggcccaggc
     5881 gggcgtgatt ggctatttgg tcaagccgtt ccgcgaggcg cagcttgccc ccgcgattga
     5941 ggtgacgctg gcccgcttca gggagttcga ggcgcttggc aaggagctgg gggacatcaa
     6001 gtcggcaatg gaggcgcgca aggtggtgga acgcgccaag ggcctgctca tggaccgctt
     6061 cggcctcagc gagagcgagg ccttccgccg catccagaag cgcagcatgg acagccgcaa
     6121 gccgatgcgc gacatcgcgg aggccatcct actggcaagt gagatggaga aagacagtta
     6181 ggtaactaca aattacggat tacgaattac aagtgataag tagagaggcg acaagcggct
     6241 ctcagggacg agttactgtg agagccgctt tgcatcttac ccgtaattcg taattcgtaa
     6301 tgacttacgc ggtgccgacg aggttgaagc caaatatcac caagaggatt gtaagcagcg
     6361 ccagggtgac cacggaaaag attaccgcta cgcgccgctt aatgggcttc atttgggcga
     6421 agcgctcgcc gagggccttg gagtcggtga tgcccttgag ccaggccgtg tcgatggcgc
     6481 gttcgaggtt gggcctgagg ccgtacagcc ccgcgcaggc cgaggcgacc agcagccacc
     6541 attcgaggtc cgacatcgtg aaggcgacca tcgccaccgc ccacagggcc gcgaaccagc
     6601 cccaggtgag aaagagcgtc agcgtctgga gcaccgcgta gacggtgagg cttttctggt
     6661 tgctcgtgtt gttgcgattt tcaggaacct gcttcattcg tttacctctg gggagtaatg
     6721 agtaatgagt gatgaggact gaggactgag gactgagcag cactaaccca aaccaccatc
     6781 ctatttgcgc actgcttctt cactatcccg tctcttaact cagtcctcag tcctcattac
     6841 tcatcacttc cctagccttt gttgaatttt cgctttagat ccgccaggtc ggtgttgacc
     6901 tgattttcgg tttcgaggtc ggagaagcgg gagtcgaggt cctgtccgct tagttcggcc
     6961 atagcagagg ccctggcgag acgctggtcc acctcgtttt ccatgcgctg gaggctggag
     7021 tcggcggtct ggccctgcac gttctggagg gcgttggtga tggcctcctg ggtggcggcg
     7081 cggcgctgct tggcgatgat aatctcgcgc ttggtctcgg cctcggatat tttggcctca
     7141 agcttggcga gggcctcgcg caggtcgcca acctggtcgt gctgcgactc ccactgggcc
     7201 tggtaggtgt cggcgagctt ctgcgcgctg gcgcggcggg tgagggcctc gcgagcgagg
     7261 tcgtcctggt cggcctgcac cgccatctcg gccttgcgct gccagtcgtc cgcttcgctc
     7321 ttgaagcgtt cgtactgcga gcggacgcgg gtctcgtcgg ccattgccat cgcggtggcc
     7381 gcgcgcgctt cggaaagctg ctcggtcatc tggcgcaggt actcatttac catcttctcg
     7441 gggtcctcgg cagcgtcaat catggcgttg atattggcgt tgatgagggt ggcgatacgg
     7501 gtcagtatgg acatcgttga tcctccagcg ggttgtaatc aataaacagg ggcgggccgc
     7561 tactacgcgt ggcaatcgac gtgccagccg cgccaggctc ttggcaatag cgggtccatt
     7621 ttatcatacg cgggcaggct ttgttagcgt cccatgatag tatacgcacc cggcgcgcct
     7681 atgttacatg caggcagggc tagggtccga ccgtcaccag gccgcgcaac tgggagagta
     7741 cgtgctcttt gatgccgggc acgcgcgcca ggtcgtcaac cgtcctgaac ggcccgtttg
     7801 cctggcggtc gttaatgatg cgcgcggcga gcacctcgcc aatgccgggt agctcttcca
     7861 gcgcctgggc gttggccgtg ttgacgttca ccctgggagg gggagcgccc gtaacggccg
     7921 gctgtgggga agggggcgct tcatcgccgg tgacgctagc accaggcgct ccaccctggc
     7981 cctttgtctc atcttcggcg gcctggaccg gctcgccctc gcggggcacg gagatatgct
     8041 gttcgtcggc tatgcgagcg gccaggttga tgccctcacg gttggcactg tccgtgaagc
     8101 cgccggaagc cgccacggca tcggcaaggc gcgaccccag gggcagggtg tacacgccgg
     8161 ggctgacgac ctcgccgctg acgtacacca ccagggcttg cggggttggc tgggttgggg
     8221 cggaagtgga tgcaaccccg ccggcctgag tggtggcacc cggcaggccc gcctctgcgg
     8281 tgggcgtgtg cgtgggtgcg ctgctgatga gcaccgcgtt ggcgggtgcc gaagcccttg
     8341 tcgcgagcag ccacagtatg cccgccgccg ccaggacaat cagggccagc agggaccagg
     8401 caaggggtat tcgctttgca agcagcgtct tcacatggcc ctcatggcct acactccagg
     8461 tccgcccagc tggcggccac ctatcttgac cgcaagatga ccagcgaccg gggctgaagg
     8521 tccagcacga tgtcgccggc gttgcggggt gacagggtgc gcaacgtgct gtcacccgtg
     8581 cgggcggggt tgttgttctg gcggtcgaag agggtaaggt cgaactcagc gtccgcgaat
     8641 gtgtcgggta atgtgacctt gacgcgggcc ggggccaagc ccgtgtacca caggaagagg
     8701 gtagcgttgg cggggtggcc gtacgccagc agaccgagcg tgccgtccgt aggagcgttg
     8761 cctgagcgta caggcagttg gccgcccgga cgctcctggt aggccttgta ggcgtggtac
     8821 acgggcttgg cgaggccgtc gtaggtgagc atgccccacc tgccccagta ggacctgggg
     8881 ccgatgccgt cttttagctc gaacaggagc gccttatcca gcctctcgcg ctgcatgctc
     8941 tcgattgagg agaggaaggc aatcgcgccg tccgccgtgt cggtggcccc gttggcgctg
     9001 gtgtcgaggg ggccgccttg cagcacgttg aactcggtga tgattagctc cgggctgaac
     9061 tgcgggtact tcccggcgat ttcgcgcacg gcccggatgt tggcggccaa ttcctccggc
     9121 tgcctgccgt aagagtgcca cgacacaaag tcgatacggc ctttgacgcc caggctggct
     9181 tcatgctgca tgaactcgtt gatgtggtcg ggctggtagc tggcgaccgc ggggccgccg
     9241 atgcggatgt tggggtcggc ggcgagcgcg gcttccaccg tcacgtcgta caatcgcagg
     9301 tactcggggt agctgccctg ccagaagtcc cagaggttcg gctcgttcca gacctcccag
     9361 tagcgcacgc ccagccgccg ttcgccgttg acgtgccgca ccgtggccgc gaccaactcg
     9421 gcccattcct cgtaccgggc ggggggcacc acgcggctgc taccgctcac cgacatcgtt
     9481 tcgggcatgt acgacaggca gatgaacggc tccttgccca tcgctttgat gccgtccagc
     9541 acgcggtcga aatcgtccca tcggtaggtc agcttgccgt caggagcgcg tccgactatg
     9601 ccgtagtagt tgaagatgtt gtcgaggcgc acgaggcccg gccccaggtc gcggcccagc
     9661 tcgcggacgt tgtcgaaaaa gcccgcgtcg tccgattcgc cgccctgggc gatgcctatc
     9721 tccagcttgc cctccggggc gggggcatag tcgacctgca actcgtaccg tggggcttcg
     9781 gcgtttttca aggcttcgag gccgaggtgc ccaagcagta cttcgtggtc cggggcgagc
     9841 tcggggtggt actcgaaccg cgcccgctcg aaccactgct gttgcaggcc ctgctcgtcc
     9901 gcgaataggc gcgagatggg gtagccgaac acccggaggc cgccattgtc ccgccagtac
     9961 gagaggaacg gctccgcgac ggtctgccct gtttcgggga agaagtggcc ggttgtggag
    10021 gtagcatcct ggtctgcctg gctcgtgtgg gcggctgcgt gcaactgggc gcggaaggtg
    10081 gggccgctgt acccggcggt aagctctctg cccaggaggc cgagcagcac ctcgtggggt
    10141 gtgcccgcgt gctcagggtg gtgctccagg cgctggcgct cggtccactg cacgaggtag
    10201 ccattttcga ggcgggccgg cgtcacgggg tagccgaaga ggagcacgcc gccctgtgcg
    10261 tcgtagtagt cgaggaattc gggagcgagg gtgaggccgg tctgctcgta gtagcggcct
    10321 tccagggcgg cggacgcgta ggcggcaggc
//
LOCUS       DASRWZ010000181         6392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_14875,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000181 DASRWZ010000000
VERSION     DASRWZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14875"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /locus_tag="VFH60_02745"
     CDS             complement(<1..1786)
                     /locus_tag="VFH60_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6312727.1"
                     /translation="MVTRLGDRLVAARRRRFVGRENERELFRSALTAPELPFVVLHVY
                     GPGGVGKTTLLKEFIAVAAELGIPTLYVDARNVDPSPEGVMAALQLALGLTSPENPVD
                     VMAERGGRQVLFFDTFELLAPLVTWLREVLLPQLPDDTLIVFGDRRPPVPAWYTDPGW
                     QDLIHILALRNLSPNESKDYLSRRNIPAEHHDEVLTFTHGHPLALSLVADVFAHKGDM
                     RFQAEATPDVVKALLEQLVQKVPGPSHRTALEVCALVRVTTEALLSAVLDTPDVHELF
                     DWLRGLSFIESGPLGLFPHDLAREALTADLRWRNRNWYSELHRRIRNFYTSGLEKASL
                     LEQQRILFDYIFLHRDNPVVRPYLEWQESGTSVPGAMREDDEPRLVEMVREFEGDESA
                     QIAAYWFQREPAGVLVFRDTEQNPTGFLLSLSLEQLTDADAEVDPAIDRARRFLSLKA
                     PLRTGEAARHFRFWMASDTYQSVSPVQSLIFVNAVRQYFTPGLVYSFFPTADPAFWAP
                     LFAYADLHPVPEAEFEAGGRRYGVYGHDWRAVPVMAWLSLLAEREIAAAPEQVAHAPA
                     PSPVVVLSEQEFESAVQEAMRNYARPEVL"
     gene            complement(2133..4274)
                     /gene="glgP"
                     /locus_tag="VFH60_02750"
     CDS             complement(2133..4274)
                     /gene="glgP"
                     /locus_tag="VFH60_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET6312728.1"
                     /translation="MNLIQPAGTLKVLPERIGRLNELAYNLWWSWHPEAQRLFSEVDP
                     VLWESVYHSPVRFLNDVRRRSLDVAAERAEYLDLYDMVMAKFDKYLGARDTWYGANHG
                     GEEGPIAYFSFEFGLHESLPIYSGGLGVLAGDHVKGASDLGVPMVFVGYLYPQGYFRQ
                     IINQDGWQEAEYPKIDFDDKPVLPAQTPDGREVIVSVELPGRTVFAKVYRFVVGRVSL
                     YMLDTDIHPNAPSDRELSSRLYGGDQDMRVAQEMVLGIGGVRALRLLGIEPRAWHMNE
                     GHSAFLLLELARERVEKGEQFQQALDNVAASAVFTTHTPVAAGNDAFSFDLISKYFTD
                     YAPLLGISKEQLFDLAKEGQPWGPAFSMTALALRGSTYHNGVSKLHSRVSRNMWHWLY
                     PNVPVEQVPISAITNGVHTGSWLAADLHGFFDRYLGEGWYDRLDDPGVWQPLYGASDQ
                     ELWEIHRRLKHALVRFTRERMSAWYSRNGGPTDVTNLLDPEALTLGFARRFATYKRAT
                     LIFNDVGRLMSIISNPERPVQMVFAGKAHPKDEPGKHFIQQVNWAARHEGLKGKIIFL
                     EEYDMNVARYLVQGVDVWLNNPRRPYEASGTSGMKAALNGVPNFSVLDGWWAEGYNGK
                     NGWAIGEGQEYGNPDEQDWHDVNSLYQTLEHQIVPAYYNRDAGGVPTEWVRLMKEAIV
                     SCAPTFSMHRQVKEYTETFYLPAMAASGGGK"
     gene            complement(4555..5811)
                     /gene="fabF"
                     /locus_tag="VFH60_02755"
     CDS             complement(4555..5811)
                     /gene="fabF"
                     /locus_tag="VFH60_02755"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015844186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET6312729.1"
                     /translation="MSAANTGLKRVVITGLGAITPLGHGRRGLWAGVLAGRSAVKRMD
                     RFDPGPYRSQVAAQIDDFDPLDHLEAQRARRLDRYSQFALAAAQQAVLDSGLCLSASD
                     AEHSGVFLGSALGGLGFAEEQHDAFVRHGLRGVNNILALTVFGSASSCNVAMEMGFHG
                     PNQTNANSCASGAVAVGEAFNLVRLGRARVMLAGGVEAPLAPLTFGAFDLIRAISSNS
                     NDEPSRASRPFDAERDGFVMSEGSAVLVLEEYEHARARGATVYAEVLGYSTTTDAYHM
                     TAPRPDGTQASRAMREALADAHLRPEEIDYISAHGSSTPLNDKTETLAIKQVFGERAY
                     SIPISGTKSMHGHALGASGAFELAITCLAMEHDYLPPTANLHHADSECDLDYLPNAGR
                     SARVHAVLSNSFGFGGINACLVLGRA"
     gene            complement(5863..6327)
                     /locus_tag="VFH60_02760"
     CDS             complement(5863..6327)
                     /locus_tag="VFH60_02760"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6312730.1"
                     /translation="MHTSNEITIHAPYERIFALASRVEEWGSILPHYRYVRVLKQAGN
                     RKWVRMSAWRNFIPVTWSAIETVEPGTADEPGRILFHHIKGLVRGMDVEWSFQPQADG
                     SYLVKISHQLDRPPFPTRILGPRLIELVVGRGFIGYIAGKTLRRIKHLAERG"
BASE COUNT         1164 a         2076 c         1989 g         1163 t
ORIGIN      
        1 tcaggacctc cggcctggcg tagttgcgca ttgcctcctg cacggcgctc tcgaactctt
       61 gctcgctcag gacaaccacc ggggagggtg cgggtgcgtg tgcaacctgc tccggggcgg
      121 cggctatctc gcgctcggcc agcagcgaca gccacgccat caccggcacc gcccgccagt
      181 cgtgcccgta cacgccatag cgcctgcctc ccgcctcgaa ctcggcctca ggcaccgggt
      241 gcaggtcggc ataagcgaac aagggtgccc agaaagccgg gtcggcggta gggaagaacg
      301 agtagaccag gccaggcgtg aagtactggc gcacggcgtt cacgaatatg aggctttgca
      361 ccggcgacac cgattggtac gtatccgaag ccatccagaa tcggaagtgc ctggccgcct
      421 ctccggttct cagcggtgct ttgaggctca ggaagcggcg ggcgcggtcg atagccgggt
      481 ccacctcggc atccgcgtcg gtaagctgct ctagcgacag gctcagcagg aagccggtag
      541 ggttctgctc ggtgtcccgg aagaccagca ccccggcagg ctctcgctgg aaccagtagg
      601 cggcaatctg cgccgactcg tccccctcga actcccgcac catctcgacc agcctcggct
      661 catcgtcctc ccgcatggcc cccggcacgg acgtacccga ctcttgccat tcgaggtaag
      721 gccgcaccac cgggttgtcg cggtgcaaga atatgtagtc gaacaggatg cgttgctgct
      781 ccagcagaga ggccttttcg agaccgcttg tgtagaagtt gcggattcgc ctgtgaagct
      841 ccgagtacca gttgcggttg cgccagcgca ggtcggcggt gagtgcctcg cgggccaggt
      901 cgtgcgggaa cagccccagc ggccccgact cgatgaacga cagcccccgc agccagtcga
      961 aaagctcgtg cacgtcgggc gtatccagca ccgcgctcag cagggcctcc gtggtgacgc
     1021 gcaccagggc gcacacctcc agcgcggtgc ggtgggaggg gccgggcacc ttctggacga
     1081 gctgctctaa cagcgccttt acaacgtcgg gggtggcctc ggcctggaag cgcatgtccc
     1141 ccttgtgcgc gaacacgtca gccacgagag acagggcgag cgggtgcccg tgggtgaacg
     1201 taagcacctc gtcgtggtgc tcggcgggga tgttgcggcg tgacaggtag tctttgctct
     1261 cgttggggct gaggttgcgc agcgccagga tgtgtatcaa gtcctgccag ccggggtcag
     1321 tgtaccacgc gggcacgggg ggcctgcggt cgccaaacac gatgagcgtg tcgtcgggca
     1381 gttgtgggag cagcacctcg cgcagccagg taacgagcgg tgccagtagc tcgaacgtgt
     1441 cgaagaacag cacctgcctg ccgccgcgct ccgccatgac gtccaccggg ttctcagggg
     1501 aggtcagccc cagcgcgagt tgcagggccg ccatgacccc ttcgggtgag gggtccacgt
     1561 tgcgggcgtc cacgtaaagc gtcgggatgc ccagctcggc ggccaccgcg atgaactctt
     1621 tgagaagcgt tgtcttgccc acgccgcctg gtccgtacac gtggagcacc acaaacggaa
     1681 gctccggggc ggtcaaggcc gaccggaata gctctcgttc gttttccctc ccgacaaaac
     1741 ggcgacgccg cgccgccaca agacgatcac ccaggcgtgt aaccatgcga cattcctgct
     1801 actaatatta ggagcgcaat cgatgtgcgc attatactgc gattcaagtc tatgtacaac
     1861 tgccagtaaa acaatgtatg tgtcgccatc gctcggtgca aaatccagct attgcggcat
     1921 aaggaacacc gagaataccg aagcgccaag tcgtcaagac gccaaggacc acgccaagat
     1981 agaggacggt gttctaagca aaaggaccag ggtacgacat catgtaccct ggtccttttt
     2041 gtacccacca aacctttatt ctctttgcgc gtcctttgcg tactttgcgt ctttgcgttt
     2101 tcgtactctt ggcgtttcgg tctaagaagt tgctatttgc cccctccgct ggcagccatg
     2161 gcggggaggt agaaggtctc ggtgtactct ttcacctggc ggtgcatcga gaaggtcggg
     2221 gcgcaggaga caatcgcctc tttcatcagc cggacccact cggtgggcac gcccccggcg
     2281 tcgcggttgt agtaggcggg cacgatttgg tgttccaaag tctggtacag ggaattcacg
     2341 tcgtgccagt cttgctcgtc ggggttgccg tactcctgcc cctctccaat ggcccaaccg
     2401 ttcttcccgt tgtacccctc ggcccaccag ccgtcgagca ccgaaaagtt aggcacgccg
     2461 ttgagggccg ccttcatgcc gctcgtgccg ctggcctcgt agggcctccg cgggttgttg
     2521 agccacacgt ccacgccctg cacgaggtaa cgcgccacgt tcatgtcgta ctcttcgagg
     2581 aagatgatct tccccttcag cccctcgtgc cgcgcggccc agttcacctg ctggatgaag
     2641 tgcttgcccg gctcgtcctt cgggtgggcc ttgcccgcga acaccatctg caccggccgc
     2701 tcagggttgc ttatgatgga catgagcctg cccacgtcgt tgaagatgag ggtcgcccgc
     2761 ttgtaggtcg cgaacctgcg ggcgaaccca agtgtgaggg cctccgggtc gagcaggttg
     2821 gtcacatcgg taggtccgcc gttgcggctg taccaggcac tcatgcgctc gcgggtgaac
     2881 cggaccagcg cgtgcttgag cctgcggtga atctcccaga gttcctggtc ggacgcccca
     2941 taaaggggtt gccatacgcc ggggtcgtcc agccggtcgt accagccctc tcccaggtag
     3001 cggtcgaaga agccgtgcag gtccgccgcc agccacgagc cggtgtgcac gccgttggta
     3061 atggccgaaa tgggcacctg ctccaccggc acgttagggt agagccagtg ccacatattg
     3121 cgcgataccc ggctgtgcaa cttgctcacc ccgttgtggt aggtggagcc gcgcaacgcc
     3181 agggccgtca tgctgaaggc gggaccccag ggctggcctt ccttcgccag gtcgaacagt
     3241 tgctccttgc taatgcccag cagaggcgcg tagtcggtaa agtacttgga gatcaggtcg
     3301 aaggagaaag catcgttccc ggcggccacg ggagtgtgag tcgtgaagac ggccgaggcg
     3361 gccacgttgt ccagcgcctg ctggaactgc tcgcctttct cgactcgctc ccgtgccagt
     3421 tcgaggagga ggaaggcgga atggccctcg ttcatgtgcc aggcgcgagg ctcgatgcct
     3481 agcagccgca gggcgcgcac gccgccgatg cccagcacca tctcctgcgc cacccgcata
     3541 tcctggtcgc caccgtacag acgcgacgac agctcgcggt cggagggggc gttcgggtgg
     3601 atgtcggtgt caagcatgta aagcgacact cgccccacga cgaagcggta gaccttggcg
     3661 aacaccgtgc gccccggcag ctccaccgac acgatcactt cgcggccgtc gggcgtctgc
     3721 gcgggcagca cgggcttgtc gtcgaagtcg atcttggggt actcggcctc ctgccagccg
     3781 tcctggttga tgatctggcg gaagtagcct tgcgggtaga ggtagccgac aaacaccatc
     3841 ggcacgccca ggtcggaggc cccttttacg tggtcccccg ccagcacgcc caggccgccc
     3901 gaatatatgg gcagcgactc gtgcaagccg aactcgaagg agaagtaggc gattggccct
     3961 tcctcgcccc catgattcgc gccgtaccag gtgtccctcg cgccaaggta cttgtcgaat
     4021 tttgccatga ccatatcgta caagtcgagg tactcggccc gctcggcggc cacatcgagg
     4081 ctgcggcggc gaacatcgtt gaggaaacgt accggactgt ggtacacgga ctcccacagc
     4141 acgggatcga cttcggagaa gaggcgctgg gcttcagggt gccagctcca ccagaggtta
     4201 taagcaagtt cgttcaggcg gccaatacgc tcaggcagta ccttcaaggt gcctgcgggc
     4261 tggatcagat tcaagagaaa accctccgtt tatgctttcg ggcggttcta cccacctcgc
     4321 attttattag atgcgcccat tctttgccac caagccgaga tatgcacata gcccctcggc
     4381 cggcgacgcg cccctacagt tgcgtccttc ccgcctaccc cgaacaacgc agagcggcag
     4441 gccagtatgc agtcaacact gacccgctaa ctccatcgcc actgatagtg agttgccatc
     4501 taagatgagc aacgactatg ccacgccccg gtaagcgctg cagtcgcgca cccgctaggc
     4561 cctacccagc accaggcagg cgttgatgcc gccgaagccg aacgagttcg atagcaccgc
     4621 gtgcactctc gcgctacgac ccgcgttagg gaggtaatcg aggtcgcact cagagtcggc
     4681 gtggtgcaga ttggccgtgg gcggcaggta gtcgtgctcc atcgccaggc aagtaatggc
     4741 tagctcgaac gcaccactcg ccccaagcgc gtgcccgtgc atcgacttgg tgccgctgat
     4801 aggaatggag taggcgcgct ccccgaacac ctgcttgata gcgagcgtct ccgtcttgtc
     4861 gttgaggggg gtgcttgagc cgtgcgcgct gatgtagtct atctcttcgg ggcgaaggtg
     4921 cgcgtccgcc aatgcctccc gcatcgccct acttgcctgg gtgccatcgg ggcgaggggc
     4981 cgtcatatga taggcgtcgg tggtggtcga ataacccagc acctccgcgt agacggtcgc
     5041 ccctctggcg cgagcgtgct cgtattcctc aagcaccagc accgccgacc cttccgacat
     5101 cacgaagccg tcccgctcgg cgtcgaaggg gcgggaggca cgcgacggct cgtcattgga
     5161 gttgctggat atagcgcgga tcaggtcgaa cgccccaaaa gtcagcgggg ccagcggcgc
     5221 ttcgacaccc ccggccagca tgacccgcgc ccggcccaga cgcaccaggt tgaacgcttc
     5281 gcctacagcc accgcgcccg aggcgcacga gttggcgttg gtctggttcg gtccgtggaa
     5341 tcccatttcc atcgccacgt tgcaggagga agccgagccg aacacggtga gggcaagtat
     5401 gttgttcacg ccccggaggc cgtgccgcac gaacgcgtca tgctgctctt cagcgaatcc
     5461 aagcccgccc agcgcgctgc ccaggaacac ccccgaatgc tcggcgtccg aggctgagag
     5521 gcacaggccc gaatcgagca ccgcctgctg cgccgccgcc agcgcgaatt gagagtacct
     5581 gtcgagccgt cgggcgcgtt gggcctccag gtggtccagc gggtcgaagt cgtctatctg
     5641 cgccgccacc tgcgacctgt aggggcctgg gtcgaacctg tccatgcgct tgaccgccga
     5701 cctgcccgcg agcacccctg cccacaggcc gcgccgcccg tgccccaggg gcgtaatcgc
     5761 gcccaggccc gtaataacca ctctttttag cccggtattc gctgctgaca aagaaaagct
     5821 ccgaatctat aaccctgtac gcacgccggt tgcacgagca aatcagcccc gctcggcgag
     5881 gtgcttgata cggcgtagtg tcttgcccgc gatgtagcct ataaagcccc gccccaccac
     5941 aagctcgatc agcctggggc cgagaatgcg cgtagggaaa ggcggcctgt cgagttggtg
     6001 gcttatcttc accaggtagg aaccgtccgc ctgtggctgg aatgaccact ccacgtccat
     6061 cccgcgcacc agccccttta tatggtggaa caagatgcgc cccggctcgt cggccgtgcc
     6121 tggctccacc gtctctatcg cgctccacgt caccgggatg aagttgcgcc acgccgacat
     6181 gcgcacccac ttgcggttac ccgcctgctt gagcacgcgc acgtacctgt aatgcggcag
     6241 tatgctaccc cactcctcga cccgcgaggc aagcgcgaag atgcgctcgt atggggcatg
     6301 gatggtgatt tcgttgctcg tatgcatagt tttcagggcc tcagcagcga ccagagaaac
     6361 attggactta gggcggcgcg ggcatcggca aa
//
LOCUS       DASRWZ010000182         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_700064, whole genome shotgun sequence.
ACCESSION   DASRWZ010000182 DASRWZ010000000
VERSION     DASRWZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700064"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFH60_02765"
     CDS             <1..484
                     /locus_tag="VFH60_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6312731.1"
                     /translation="PSRSRLLREAIDDAADKGVVTAAAAGNSNITVPQYPAAGTGVAA
                     AVDGLLAVTSVDAYEKKSHFANYGLWVDIAAPGEGIRSAFPVSVYASWSGTSMATPFI
                     AGQAALVHAVDGSLGPAGVERRIRCSARPLALKNPTYVSMLGAGHADVRASIDPGACR
                     "
     gene            729..1298
                     /locus_tag="VFH60_02770"
     CDS             729..1298
                     /locus_tag="VFH60_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6312732.1"
                     /translation="MREEDMRPAFESDRDVIQGCRAGDAGAWQSLLNRYERLVFSIPR
                     RYGLSREDADDITQLTFTILIQSIGKLPDDSNLGAWLATVARRHTWRLFNRSRREGAG
                     EYTDSSEATPLLPNSATEDMAQWELTEWLHHGLSLVGEPCRGLLHALYLEPAQPSYAV
                     VAARMGMAVGSVGPTRIRCLKRVKEVLDE"
BASE COUNT          297 a          452 c          481 g          278 t
ORIGIN      
        1 gcccagccgg tcgcggttgc tccgagaagc gattgacgac gctgccgaca agggcgtcgt
       61 caccgctgct gccgcgggca actcgaacat cactgtgccg cagtaccctg cggcgggaac
      121 cggcgtcgcg gccgccgtag atgggctgct ggccgtcacc tcggtggacg catacgagaa
      181 aaagtcgcac ttcgccaact acgggttatg ggtggacata gccgcgcccg gcgagggtat
      241 ccgcagcgcc ttcccggtca gcgtgtacgc ctcctggagc gggacctcta tggcaacgcc
      301 gttcatcgcc ggccaagcgg cgctcgtcca cgccgtcgac ggctcgctgg gccccgcggg
      361 cgtagaaagg aggatacggt gtagcgcccg gcccctcgcc ttgaagaacc cgacctacgt
      421 cagcatgcta ggcgccggcc acgccgatgt acgcgccagc atcgacccgg gtgcttgccg
      481 ctaattcacc gagtaggttg gtccggtact gcagggcatc aaatcacgct catgttctta
      541 gtatagactg tagcaatgac cctgctcgag cctggctagt atgatctgct ttcgtcagaa
      601 gtgcgccctg ttgggaagaa gagatgtgca cgagtctaac caaaacgata gtcttggtac
      661 cctctggttg ggtctttggc tgcgagccgg cgaaagcaca gagcgcgagc ctccggggtc
      721 ggtggaccat gcgtgaggaa gacatgcgtc ccgcgttcga gtcggatcga gatgtaatac
      781 agggctgccg tgcgggcgac gctggcgcat ggcaaagttt gctgaatagg tacgagcggc
      841 tcgtgttctc gatccctcga aggtacgggc tctcccggga ggatgctgac gacatcacgc
      901 agcttacgtt cacgattctg atccagagca taggcaagtt acccgacgac agcaacctgg
      961 gcgcgtggtt agctaccgta gcccgccgcc acacctggcg ccttttcaac cgcagtcgtc
     1021 gcgaaggtgc cggcgagtac actgattcga gcgaggccac gccgctgctc ccaaacagcg
     1081 ctaccgagga catggcgcaa tgggaactaa ccgagtggtt acaccatgga ctctcacttg
     1141 tcggcgaacc ttgccggggc ctgctacatg ccctctacct cgaaccagca cagccgtcat
     1201 acgccgtagt cgccgcgcgt atggggatgg ccgtgggcag cgtaggcccc acccgaatcc
     1261 gctgcctgaa gcgcgtgaag gaggttttgg atgaatgatg gcaagcgaat cagggcgaac
     1321 aggggcacgg atggttgccc gcagccgact acaattcttt ctcgggccgg tacgaaattc
     1381 tgtatttttc tgtgcaccgg taggcatgtc caaggtagaa ggaacatgag aacactccgg
     1441 aggttgccag ccaatgcctg agcctgagag ggaggtcagc ttcgcccggc tcgtcgactg
     1501 ggtggaag
//
LOCUS       DASRWZ010000183         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_974248, whole genome shotgun sequence.
ACCESSION   DASRWZ010000183 DASRWZ010000000
VERSION     DASRWZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974248"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFH60_02775"
     CDS             complement(<1..475)
                     /locus_tag="VFH60_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312733.1"
                     /translation="MKVTEPRRFMWSITAMLAIMLGVVLFVGSAFASLPTKQAQSKSS
                     NAPSGGDTATATLTATLTVTSTATLTATATVQPSATLTSTATLTATSTVTSTATLTAT
                     STVTSTNTVVASATTTPSRTSTGTATGTASATRTAPGTGTATQTRTATVVASQTPG"
     gene            1079..1765
                     /locus_tag="VFH60_02780"
     CDS             1079..1765
                     /locus_tag="VFH60_02780"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6312734.1"
                     /translation="MRARLRKIAFESLYGPFAWLYDWVSKTFFLGQWRNWQRAAIPHV
                     RGRRVLEVGMGTGNMQLDLRHLGYDVWGVDLSPQMLRQAAKKARRRNLPPWWACRARA
                     EALPFPPACFDTVVSTFPSEYIIGEATLAEIKRVLRPGGRLVVVPAGWITPKDAQGKA
                     FEGVARLVYGDSHGGTDAPLPARQPGAPQNTGWVALMEGRLREAGFDPSTYIASNARG
                     SAVVVVADRP"
BASE COUNT          343 a          565 c          598 g          344 t
ORIGIN      
        1 tgccgggcgt ctggctcgcc acgacagtag ccgtcctcgt ctgcgtggcc gtgcctgtgc
       61 caggagcggt acgggtcgcc gaagcggtac cagtagcggt gccggtggag gtgcggctcg
      121 gggtggtggt ggcgctggct acaaccgtgt tggtgctggt caccgtgctt gtcgccgtca
      181 acgtggctgt gctggtgact gtgcttgttg ccgtcagcgt ggctgtgctg gtgagtgtag
      241 ctgaaggctg cacggtggcc gtggcggtca gcgtggcggt gctggtgact gtgagcgtgg
      301 cagtcagggt agcggttgcg gtatcccctc cgctgggagc gttagatgac ttgctctgcg
      361 cctgcttggt gggcaagctc gcgaacgcgc tgcctacgaa cagaactaca ccgagcataa
      421 tggcaagcat cgctgtgatg ctccacatga atctccgtgg ttcggtaact ttcatttgtg
      481 ttcctcctga ccttgcgatg aacgatgaga gcgtgcgcag ccggcaactt tgctgctacg
      541 caggcagcat gacccgtttc gcctaccagg ttggttatca tccgtgggtg agtgaaggat
      601 attggtagaa cgtatggctg ggtcgatgta ggaccaagac gacatcgacg ggccgacgat
      661 gtgtctcggt aagctaaatg gggcgggtac ccggggaggc ccgcataaga cagaaggagc
      721 agcaatgcgc ccagctaaca acctctgcca tgcgcccggt acggcgagag ccatcccggc
      781 gaggtaaaga acaccttcgc agcatacgcc cggtgccgca cccgcgtcat cgcggtgaga
      841 ggaacgtgca aacacactat gctgtcaaaa gaccccaggg ttacccttcc ctactacata
      901 gcatgctaca tgccaggctc gcgaattccg gtccattccc ctggtcacga gagccaatat
      961 ttatacttgt taacatctcc cccgagccgt gtcctatagg gcactgcccg gccacaactg
     1021 atacaatggc ctcatggaca ggatagacca acgcagaaaa cgcccccgtt tagagcaaat
     1081 gcgcgccaga ctgaggaaaa tagccttcga gtcgctgtac ggccccttcg cctggctgta
     1141 cgactgggtg agcaagactt tcttcctggg ccagtggcgc aactggcagc gggcagccat
     1201 accccacgtg cgcggcaggc gcgtgcttga agtcggcatg ggcacgggca acatgcagct
     1261 agacctgcgc cacctggggt acgacgtgtg gggcgtggac ctgtcgccgc agatgctccg
     1321 gcaagccgcg aagaaagccc gccgccgtaa cctcccgccc tggtgggcgt gccgcgcccg
     1381 cgccgaggcc ctccctttcc cgcccgcctg cttcgacacg gtggtgagca ccttccccag
     1441 cgagtacatc atcggagagg ctaccctggc cgagataaag agagtgctac ggcctggcgg
     1501 caggctggtg gtagtgcccg cggggtggat cacgcctaaa gacgcccagg gcaaggcgtt
     1561 cgagggagtg gcccgcctcg tctacggcga cagccacggc ggcaccgacg ccccgctacc
     1621 ggcacggcaa ccgggcgcgc cccaaaacac cggctgggtg gccctcatgg aaggcaggct
     1681 gcgggaggcg ggcttcgacc ccagcacgta catcgcctcc aacgctcgcg gctcggctgt
     1741 agtagtggtc gcagacaggc cttgaggccc tgaatgcaag ccccgctgtc gtcctgtatc
     1801 cccatctgtc atactgagcc tcccttgtca tcctgagcgt agcgaaggat
//
LOCUS       DASRWZ010000184         4196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1043103, whole genome shotgun sequence.
ACCESSION   DASRWZ010000184 DASRWZ010000000
VERSION     DASRWZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043103"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFH60_02785"
     CDS             <1..1059
                     /locus_tag="VFH60_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6312735.1"
                     /translation="VGDPPAPPETLAPPVPQAQPTLSGTVPAPVAQPPAVALDPDPYS
                     LTVVIPAYNEGDAIHRVIGKVRELRPRAEILVVDDGSKDNTAEVALAAGARVVVHPYN
                     KGNGAAVKTGLRNATGDVVLLLDADGQHPPEDIERVLEPIGEYDLVVGARTRDSESAW
                     IRDFGNFIFNRLATYLSGRDIPDLTSGFRAMKRDMIMEFIHLLPNLYSYPTTSTLAFI
                     KAGYNVRFVPIRARKGTGRSNTRIVRDGVRAVVIILRMITLFAPMKIFLPFSIVLFLG
                     GMLYGLVNILIWGINRIPNGSVLLILSSLFIFLFGLISEQIAAMRFESSQRNWTRDHG
                     TSARDGHGDHTAKGGDGS"
     gene            1049..2104
                     /locus_tag="VFH60_02790"
     CDS             1049..2104
                     /locus_tag="VFH60_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6312736.1"
                     /translation="MAAKADPGHADPTPLSHPPTAEQHAPAHGQPGPPHVSQPSAPRS
                     RTRAWLLRIAGTLLFLALLVWLDLSGALPLEKVWSALAGADFRLVALSIAFYVPFLAV
                     KAARWRIVSASMNVPLSWGNAWRIYGIGLAAGTFTPGQAGDALKAWYVQRKGYSLARG
                     LGSSVLDRLFDVAGLAVLGLVGVLVYGGRFAGQAPVLVAWLVVCVAAVAFLAWNPTRT
                     WAVNFVARRLARLRGGGDGAGGDWSLDARTVAYAGLLTPASFAISIFRVWLLAASVGI
                     MLGPLEVSGFVGLTTAAALVPVTVGGVGTRDAIAALSFTQLGYDPAQGIAVSLLILVL
                     NVAQAITGWVIWLRYRA"
     gene            2222..>4196
                     /locus_tag="VFH60_02795"
     CDS             2222..>4196
                     /locus_tag="VFH60_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6312737.1"
                     /translation="MGIGGWASEYGVPVGAVALAIVALLALFAVLGRLGIAAGVWRAI
                     RGQWLATVGLSVLLLFATLLATIFAKGQETGQLHKYALHIVASPTNLLANGELETTSA
                     SSVQGVTGWTPLTGTIAPIAVGDAGVRLEPGAVLASSMVRVQAGATYRYSLAVPPSET
                     GTAIAQVRLLWLSGTLDLDNPVDWSDSLPRRIDYAQVPRSGEFVDGEHAAPAGATHMR
                     LELRNLGSDSLQVVKVILWVDGAQVEAHPDGRQGAVAFSFDWESAMGGAIHSKGMLKH
                     DVGAAAEHGLDMRQGADWLDDLFARNHISATFYGTGYNLLDGNTERRTFSGNPTYEWA
                     SRENRWETDYWTEHPWYGDDPYGTYQTHPAWYFGDQTRRLLAAGHEIASHTFGHMYVR
                     GSDVPSMTVDTDEWLSAAQAMGVPPPTTFAFPWRSSNSLKADMYDMLYNKGIRAVTRI
                     YALDMVDQYTVGAVKVYPTMWVMPDFLLGGASSTAGEEADGATIDADRGVQVIAEVLA
                     RRGTTSFWTHPEQLADGPAFATDRQSWQRVVEAAAHERDRGRLWIDTVASIIAYERGV
                     MSVTTRLEQGFLGIGGRQLKVDNGSGLELKGVTLTLPGDVRSASSDGVAVRTVQVAED
                     GAVRLSEPGKPTFPARQLVIDTRPPGVTTIAIEW"
BASE COUNT          689 a         1333 c         1433 g          741 t
ORIGIN      
        1 gtgggcgatc cgcccgctcc ccctgagacc cttgccccgc ccgtgccgca ggcacagccc
       61 acgctgtccg ggaccgtgcc cgcgcccgta gcccaacctc ctgccgtagc gctcgaccca
      121 gacccatatt cgctcactgt cgtcatcccg gcctacaacg agggcgacgc tattcaccgc
      181 gtgattggca aggtgcggga gcttcgcccc agggccgaga tactcgtcgt tgacgacggc
      241 tcgaaggaca ataccgccga ggtggcgctg gcggcggggg ccagggtggt ggtgcacccg
      301 tacaacaagg gcaacggcgc agcggtgaag accgggctgc gcaacgccac gggcgacgtt
      361 gtgctcctgc tcgacgccga cgggcagcac ccgcctgagg acatcgagcg cgtgctggag
      421 ccgataggcg agtacgacct ggtggtcggc gcgcgcaccc gcgattcgga gagcgcctgg
      481 atacgcgact tcggcaactt catcttcaac aggctcgcca cgtacctgtc ggggcgcgac
      541 atacccgacc tcacctccgg cttccgggcc atgaagcgcg acatgatcat ggagttcatc
      601 cacctcctgc ccaacctcta ctcctacccg accaccagca cgcttgcctt catcaaggcg
      661 gggtataacg tgcggttcgt gcccatcagg gcgcgcaagg gcaccgggcg cagcaacacc
      721 aggatagtgc gcgacggcgt cagggccgtc gtcatcatcc tgcgcatgat aaccctgttc
      781 gcgccgatga agatattcct ccccttcagc atagtcttgt tcctgggggg tatgctgtac
      841 ggtctggtga acatcctcat ctggggcatc aaccgcatac ccaacggctc ggtgttgctc
      901 attctgagca gcctgttcat cttcctgttc ggtctcatct cggagcaaat cgccgccatg
      961 cgcttcgaga gcagccagcg caactggacg cgggaccacg gcacgagcgc gcgggacggg
     1021 catggcgacc acacggcgaa gggcggggat ggcagctaag gcggaccccg gccacgcgga
     1081 ccctacgccc ttatctcacc ctcccacggc cgagcaacac gcgcccgccc acggccaacc
     1141 cggcccacct cacgtgtcgc agccctcggc accccgcagc cggacccgcg cctggctgct
     1201 acgtatcgcg ggcacgttgc tgttcttggc gctgctcgtc tggctcgacc tgagcggtgc
     1261 gctgccgttg gagaaggtgt ggagcgccct ggccggtgcc gacttccggc tggtggccct
     1321 ctcgattgcc ttctacgtgc ccttcctcgc cgtaaaggcc gcccggtggc gcatcgtgtc
     1381 ggcgagtatg aacgtgccgc tgtcatgggg caatgcctgg cgcatctacg gcattggcct
     1441 ggcagccggg acgttcacgc cggggcaggc gggcgatgcg ctcaaagcct ggtacgtgca
     1501 gcggaaaggt tactccctgg cgcgcgggtt gggcagctcc gtgctggacc gcctgttcga
     1561 cgtggcgggg ttggcggtgc tgggccttgt gggcgtgctg gtgtacggcg ggcggttcgc
     1621 gggacaggcc cccgtgcttg tcgcctggtt ggtggtttgc gtggcggcgg tggccttcct
     1681 cgcctggaac cccactcgca cgtgggccgt taactttgtg gctcgtcgcc tggcgcggct
     1741 caggggtggc ggtgacgggg cgggcggcga ctggtcgctc gatgcgcgga ccgtagccta
     1801 cgcggggctg ctcacgccgg caagcttcgc catctcgata ttcagggtgt ggctgctggc
     1861 cgcctccgtg ggcatcatgc tggggccgct cgaagtatcc ggcttcgtcg gcctgaccac
     1921 cgccgccgcc ctcgtgcctg tcaccgtggg cggggtcggc acgcgcgacg ccattgcggc
     1981 cctgtcgttc acccagctag gctacgaccc ggcccaaggc attgccgtgt ccctgctcat
     2041 cctggtgctg aacgtggcgc aggcaatcac cggctgggtc atctggctgc ggtatagggc
     2101 gtgagtaatt acgaattacg aattacgaat tagcgaaacg caaacacgaa ggacgcgcaa
     2161 agtttaggga tagtttgaat ttctgtaatt cgtaattcgc aattccccag aggggtgggt
     2221 attgggaatc ggtggctggg caagcgaata tggggtgcct gtgggtgcgg tggcgctggc
     2281 gatagtggcg ctgctggcct tgttcgctgt gctgggcagg ttgggtatcg cggcgggtgt
     2341 gtggcgggcc atcagggggc agtggctggc taccgtgggg ttgagcgtgc tgctgctgtt
     2401 cgccacgctg ctggcgacca tcttcgctaa gggccaggag acggggcaac tgcataagta
     2461 tgcgctccac attgtggcta gtcccaccaa cctgctggcg aacggcgagc ttgagacgac
     2521 tagcgccagt agtgtccagg gtgtcacggg ctggacgccg ctcaccggca ccatcgcgcc
     2581 tatcgcggtg ggtgacgccg gagtgcgcct ggagccggga gcggttcttg ccagtagcat
     2641 ggtgcgggtg caagccgggg cgacgtatcg ctatagcctc gcggtgccgc cgagcgagac
     2701 cgggaccgcg attgcgcagg tgcgtttgct ctggctgtcc ggtaccctcg acctcgataa
     2761 ccctgttgac tggagcgact cgctgccgcg ccgcatcgac tatgcacagg tgccgaggtc
     2821 gggcgagttc gtggatgggg agcacgcagc cccggcgggt gcgacgcata tgcggcttga
     2881 gctcaggaac ctgggcagcg actctctgca ggtggtcaag gtgatactgt gggtggacgg
     2941 cgcgcaggtc gaggcgcacc ccgacggtcg ccagggcgcg gtggcgttct cgttcgattg
     3001 ggagtcagcg atgggcgggg caatccactc gaagggcatg ctgaagcacg acgttggggc
     3061 tgccgccgaa cacggcctgg acatgcggca gggagccgat tggctggacg acctgttcgc
     3121 ccgcaaccac attagcgcga ctttctacgg caccggctac aacctgctgg acggcaatac
     3181 ggagcggcgg accttctccg gcaaccccac ctatgagtgg gctagccgcg agaaccgctg
     3241 ggagaccgac tattggaccg agcacccctg gtacggcgac gacccttacg gcacgtacca
     3301 gacccacccg gcctggtact ttggcgacca gacgcgcagg ctcctcgcgg cgggccacga
     3361 aattgcctcg cataccttcg ggcacatgta cgtgcgcggc tccgacgtac cttccatgac
     3421 ggtggacacg gacgagtggt tgagcgcggc gcaggcgatg ggtgtgcctc cgcctaccac
     3481 gttcgccttc ccctggcgca gcagcaactc gctcaaggcc gatatgtacg acatgctcta
     3541 caacaaaggc atacgcgccg tcacccgtat ctacgccctc gacatggtgg accagtacac
     3601 ggtaggggcg gtgaaggtgt atcccacaat gtgggtcatg cctgacttcc tgctgggcgg
     3661 ggcctcaagc acggcgggcg aggaggcgga cggggccact atcgacgcgg accgtggagt
     3721 gcaggtaata gccgaggtgc tggcgaggcg gggcactacc tccttctgga cgcacccgga
     3781 gcaactggcc gacggcccag cctttgcgac cgaccggcag tcgtggcagc gggttgtgga
     3841 agccgccgcg cacgagcggg accggggcag gctgtggatc gacacggtag ccagcatcat
     3901 cgcttacgaa cgcggcgtga tgagcgtcac cacacgactg gagcaaggct tcctgggcat
     3961 cggaggccgg caacttaagg tggataacgg ctcggggctg gagctaaaag gcgtcaccct
     4021 tacactgccc ggcgacgtgc gaagcgcatc atcagacggt gtggctgtcc gcaccgtaca
     4081 ggtcgcggaa gacggtgcgg tgcggttgag cgaacctggc aaacccacgt tccctgcccg
     4141 gcagctagtg attgacaccc gtccacccgg cgtcaccacg atcgctattg agtggg
//
LOCUS       DASRWZ010000185         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_700385, whole genome shotgun sequence.
ACCESSION   DASRWZ010000185 DASRWZ010000000
VERSION     DASRWZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700385"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="VFH60_02800"
     CDS             complement(<1..1733)
                     /locus_tag="VFH60_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET6312738.1"
                     /translation="MSHNLLRKVQAKSPGAGGLEHDPLPFEQRYRRALDNAQLRRNLL
                     NFQRSWQVSREGAFSTYAENPERAATEPVEQAHSHSHASHMPLAKGTAEFEAMRDRLA
                     AIKDEVIERLPEYVERFRKAAEARGIIIYRAADAEAANRYVLNLCRERGITHVVKSKT
                     MVSEEIELNGALEAQGIKAVETDLGEWIQQLSHERPSHMVMPAIHKSREQVGDLFTQA
                     TGREVSRTDIGEQVAVARRELRRDFLEAGLGVSGANALIAESGTVMFIENEGNARLVT
                     TLPRVHVVLAGIEKLVPDYAAAMLQLRLLARSATGQGITSYTTFLSGPPEHGKEMHIV
                     LLDNGRMKMRETPLIRDALRCIRCAACADICPPYAVVGGHVFGHIYSGAIGLVNTPFH
                     HGIEAGAGPQSLCVSCNACATVCPVGIPLPQQILKVRSMVVEEEGAPMPMRAAIEFWS
                     HPRLVDIALRAGAVASAPLRDGHFTRVERTGANLVPQLHSLTGWRTPPALAMRPARDR
                     LKQRRAYAGNPLESQARGMRVAYFIQCITDRFFPRMALSVARMLEACGVEVVVPHAQH
                     CCGLPALDAGM"
     gene            complement(1735..>1917)
                     /locus_tag="VFH60_02805"
     CDS             complement(1735..>1917)
                     /locus_tag="VFH60_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET6312739.1"
                     /translation="NIVPSLDEAGALLAELERAGHRYVSLVTGPSRTADIERVLTIGV
                     QGPKALHIIIMTEEGA"
BASE COUNT          329 a          600 c          615 g          373 t
ORIGIN      
        1 atcatccctg cgtcgagggc cggcaggccg caacagtgct gggcatgcgg cacgacgact
       61 tcaaccccgc atgcttccag catccgcgcg acggagaggg ccatacgcgg gaagaacctg
      121 tcggtgatgc actggataaa gtaggctact cgcataccgc gagcctggct ctccaggggg
      181 ttgcctgcat aggccctcct ctgcttcagg cggtcacggg ccgggcgcat ggctagcgcg
      241 ggaggggtgc gccagccggt gaggctatgg agttggggga ccaggttcgc gcccgtgcgc
      301 tctactcgcg tgaagtgccc gtcgcgtaaa ggcgcggatg ccacagcgcc tgcgcgtagg
      361 gcaatgtcaa ccagcctggg gtgtgaccag aactcgatag cggcacgcat gggcatgggt
      421 gcgccctctt cctctaccac catgctacgt accttcaaaa tctgctgggg caagggaata
      481 ccgacggggc aaaccgttgc gcaagcgttg catgatacgc acagcgattg cggccccgcg
      541 cccgcttcga taccgtggtg gaagggtgtg ttgaccagtc cgatagcgcc tgagtagatg
      601 tgcccgaaga cgtgcccgcc caccacggcg taaggcgggc agatgtcggc gcaggccgcg
      661 cagcggatgc agcggagggc atcccggatc aggggcgttt cgcgcatttt catgcgcccg
      721 ttgtcgagca agacgatgtg catctctttg ccatgctccg gcggccctga gaggaaggtg
      781 gtgtacgatg taataccctg gccggtggcg gagcgggcga ggaggcgcag ttgcagcatg
      841 gcagcggcgt aatcgggcac gagcttctct atgcctgcca gcaccacgtg cacgcggggc
      901 agcgtcgtca ccagcctggc gttgccctcg ttttcgatga acatgactgt gccgctctcc
      961 gctataaggg cgttagcgcc gctgactccc aggcccgcct ccaggaagtc acggcgcaac
     1021 tcccttcgcg ccacggctac ctgctcacct atgtcggtgc ggctcacttc gcgtcctgtg
     1081 gcctgggtga aaaggtcgcc cacctgctcg cggctcttgt ggatggcggg catgaccatg
     1141 tgagaggggc gctcgtgcga cagctgctgt atccactcac ccaggtcggt ctccaccgcc
     1201 ttgataccct gggcttcaag tgcgccattt agctcaatct cctcggagac catcgtcttg
     1261 ctcttgacca cgtgcgtgat gccgcgctcc cggcacaggt ttagcacgta acggttggca
     1321 gcctcggcgt cagcggccct gtagatgatg atacctcgcg cctcggcggc cttccggaag
     1381 cgctcgacgt attcgggcag gcgctcgata acctcgtctt tgatagccgc cagcctatcg
     1441 cgcatggcct cgaactcggc ggtgcctttc gccaggggca tgtgcgacgc gtgggagtga
     1501 gagtgagctt gctcaacagg ctcggttgct gcacgctccg ggttctcagc gtaggtagag
     1561 aacgcgcctt cgcgcgagac ctgccacgaa cgctggaagt tgagtaagtt acgcctcaac
     1621 tgcgcgttat ccagcgcccg ccggtagcgt tgctcaaacg gcagtggatc atgttcaagc
     1681 ccacccgcgc cgggtgattt ggcctgcacc tttcttagca ggttgtgaga catctcaggc
     1741 accctcttca gtcattatga taatgtgcag cgccttcggc ccctgcacgc cgatggtaag
     1801 cacccgctct atatctgcgg tgcgacttgg gccggtgacg agcgagacgt agcgatgccc
     1861 cgcacgttcc agttcggcca gcagcgcgcc tgcttcatca aggctcggca cgatatt
//
LOCUS       DASRWZ010000186         6844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_563928, whole genome shotgun sequence.
ACCESSION   DASRWZ010000186 DASRWZ010000000
VERSION     DASRWZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563928"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFH60_02810"
     CDS             <1..325
                     /locus_tag="VFH60_02810"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET6312740.1"
                     /translation="YRFAVRIMGDSAVEALDKASLEYDDISLFIPHQANTRIINSIAD
                     RLHLDPEKVFINIANYGNTSAASIPIALCEAVDEGKVKDGDNVLFVAFGSGLTSAAAV
                     VRWGR"
     gene            330..1280
                     /gene="fabD"
                     /locus_tag="VFH60_02815"
     CDS             330..1280
                     /gene="fabD"
                     /locus_tag="VFH60_02815"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET6312741.1"
                     /translation="MSDTAAVARHGAGPSATAWVFPGQGSQAVGMGKALYDLYPEVRT
                     LYGTADRVLGYSISELCFNGPADQLQRTDNAQPALLTTEIAHLTALRLRYPGEYNAPA
                     FVAGHSLGEYAALVAAEVLDFEDALMLVSERGRLMNEAGATLDQPTGMVAVLGLPDEA
                     AAQVCQEAGLDLANLNAPGQVVLSGPQEGLERAAELAKAAGARRVVPLQVSAAFHSRW
                     MRPMSEHFARYLGEAPFSEPKVPVVANVTARPAPDAQDVRRLLEMQTYSPVRWVESVE
                     YMVEQGVRTFVEIGPGKVLSGLVKRIAPDAHVVSSEELLS"
     gene            1309..1530
                     /locus_tag="VFH60_02820"
     CDS             1309..1530
                     /locus_tag="VFH60_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET6312742.1"
                     /translation="MREPLIVSDPAVMMGKPVIAGTRLTVELILEKLGAGETIEQLLE
                     SYPRLTREAILTAAAFGKAPRVSGSKEQS"
     gene            1536..1853
                     /locus_tag="VFH60_02825"
     CDS             1536..1853
                     /locus_tag="VFH60_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HET6312743.1"
                     /translation="MPKVIAEVSFSPIGTGTPSLSRYITAALAVLEAHPEVRYQLNPM
                     GTVLEGERRAVIEVIEAMNEAIFAAGAQRVGTTLKIDERRDKAASMEHKVESVMEGLK
                     RDG"
     gene            1889..3313
                     /gene="accC"
                     /locus_tag="VFH60_02830"
     CDS             1889..3313
                     /gene="accC"
                     /locus_tag="VFH60_02830"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET6312744.1"
                     /translation="MFKRILIANRGEIALRVIRACHELGVEAVVAYSEADRETLPVRL
                     ADTAICIGPAAPGRSYLHIPAVISAAEMAGCDAIHPGYGFLSENPYIAEICEKVGITF
                     VGPSSEAIQAMANKVQARKLMAEAGLPIIPGTEDALLNADEAHAVAEEIGFPLLMKAV
                     GGGGGRGMRVVNSIDELLEAYNLARSEASLAFGNPDVYLERYLYHPRHIEVQVLADQY
                     GHCIHLGTRDCSLQRRHQKILEEAPAPGLTEEQTRRIGEAAVLGAKAIGYSTVGTFEF
                     LLDELGEFYFIEMNTRIQVEHGITEMVTGVDLVKWQIRTAAGEPLTLEQKDITITGHA
                     IECRVNAEDVQRGFLPAGGPVELYLPPGGPGIRVDSHLYSGYTAPSNYDSLLGKIMAW
                     GEDRQEAIARARRALIECIIIGLPTTIPFHQKILDDPRFVQGHIHTGLVTEWLAEQLV
                     ENPASAGTSTAGSPNGRVAPGRVQ"
     gene            3333..3674
                     /locus_tag="VFH60_02835"
     CDS             3333..3674
                     /locus_tag="VFH60_02835"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET6312745.1"
                     /translation="MQDTKLGTVRIAPSVLATIVSLTASGIKGVASMSSVTRPPARVL
                     QRPNMGSAPGVSLQVRNNTIAADVYIVAEPDTNLKRLAQTIQTRVREALDQMVGMSAE
                     VVNVYIEDVSD"
     gene            3782..4273
                     /gene="nusB"
                     /locus_tag="VFH60_02840"
     CDS             3782..4273
                     /gene="nusB"
                     /locus_tag="VFH60_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET6312746.1"
                     /translation="MSTAERRQARITALQTLYEVDTTEHTAEEVMLRLFNEPMSESQP
                     EGDPLVEDVRSYARTLVEGAVEHQGDLDEIIARSAPNWPVAQMSSIDKNILRLAIFEI
                     LFDNRVPLKAAINEAVELGKRFGSDSSSRFINGVLGTVAAEHQQLRRKYNKAAPGATA
                     NQE"
     gene            4295..4540
                     /locus_tag="VFH60_02845"
     CDS             4295..4540
                     /locus_tag="VFH60_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET6312747.1"
                     /translation="MSSRQELFRRLRPLLAEALGADEDEISLDTSVYDDLNADTIEFH
                     DEVIPLIEEEFGVTIDDDEVPDLTTVRELVAYLAENT"
     gene            4572..5000
                     /locus_tag="VFH60_02850"
     CDS             4572..5000
                     /locus_tag="VFH60_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET6312748.1"
                     /translation="MANATEHLREVAVQQYGVDEAEVANKDANALTELLLGKIAMDKY
                     KVSASELEGLDRRGETQLLLQNMVSDLLKVDKSKVSPETTFSDLGADSLDMVELLMTI
                     EDVCEPFGEMKIPEEDANIGTVGEAVDRIDSYITNYVSAS"
     gene            5176..5985
                     /gene="tatC"
                     /locus_tag="VFH60_02855"
     CDS             5176..5985
                     /gene="tatC"
                     /locus_tag="VFH60_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET6312749.1"
                     /translation="MSTLSSIRQRLPFGKRNGRPPRTSRRRPDGSLDVEMPLMEHLRE
                     FRDRLIKSALTVLIFTSLSFVFVDREIEVLLGMAGGRNLIAITVTENFVAYVKVALIT
                     GIGLSMPVLVYQLFQFLAPGLTRNEKRWILISLPGVMAFFVMGVVFCYFLVLPSALEF
                     LLNFGSPQIEIQPTISEYLGFVTNFMLALGLAFETPLIIFILSKVGVATPKRLKRFRR
                     WAYVLAFVAAAIITPTPDPVNQTYVAVPIILLYELGILFAWLGRPRTKKAA"
     gene            complement(6017..6682)
                     /locus_tag="VFH60_02860"
     CDS             complement(6017..6682)
                     /locus_tag="VFH60_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET6312750.1"
                     /translation="MTDPVSSTTPGRVLSVNVGKPREIEWLGQRDTTAIWKSPVAGRV
                     PLRGVNLEGDDQADRRVHGGRDKAVYSYGREDAEWWEQEVGRSLEPGTFGENLTLAGV
                     DMNGAVIGERWEIGTALLEVAQPRFPCWKLGARMGDPDFPPRFGAALRPGVYLRILRE
                     GDIGAGDEVRSVFTPTHNVTIGDVAYIYHKDHAGASLLLEVPELPETLKGWARRMQHH
                     ARP"
BASE COUNT         1374 a         2089 c         2119 g         1262 t
ORIGIN      
        1 ttaccgcttc gcggtgcgca tcatgggcga ttcggcggtg gaggcgctcg acaaagccag
       61 cctcgaatat gacgacatat ctctcttcat cccacaccag gcgaatactc gcatcatcaa
      121 ttcgatagcc gaccgtctgc acctcgaccc ggagaaggtc tttatcaaca tcgccaacta
      181 cggcaacacc tcggcggcct ctatccctat tgcgctgtgt gaagccgtgg acgagggcaa
      241 ggtcaaggat ggcgacaacg tgctcttcgt ggccttcggc tccgggctga ccagcgccgc
      301 cgccgtcgtg cggtgggggc ggtaggggcg tgagcgacac ggcggcggtt gcacggcacg
      361 gtgcgggacc gtccgctacg gcctgggtgt tccccggcca gggttcgcaa gccgtgggca
      421 tgggcaaggc gctttacgac ctctacccgg aagtgcggac cctgtacggc acggcagaca
      481 gggtgcttgg ctactccatc tcggaactgt gcttcaacgg ccccgccgac cagctccagc
      541 gcaccgacaa cgcccagccc gccctcctca ctacagaaat cgcccatctc acagcccttc
      601 gcctgcgcta ccccggcgag tacaacgctc ccgccttcgt agcggggcac agcctgggcg
      661 agtacgcggc gttggtggcg gcggaggtgc tggacttcga ggacgcgctc atgctagtgt
      721 cggagcgcgg gcggctgatg aacgaagccg gggccacgct ggaccagcct acgggcatgg
      781 tagccgtgct tggcttgccc gacgaggcgg cagcgcaggt ctgccaggag gctggcctgg
      841 acctcgccaa cctgaacgcc cccggccagg tggtgctgag cggcccgcag gaggggcttg
      901 agcgggctgc ggagcttgcg aaggcggcgg gtgccaggcg ggtggtgcct ttgcaggtga
      961 gcgcggcctt ccactcgcgc tggatgcgcc ctatgtcaga gcacttcgcc cggtacctgg
     1021 gtgaggcccc cttttccgag ccgaaggtgc ccgtcgtcgc caatgtcacc gcccgccccg
     1081 cccccgatgc ccaggacgta cgccgcctgc tagaaatgca gacctactcg ccggtacgct
     1141 gggtggagtc ggtggagtac atggtggagc agggcgttcg cacttttgtg gagattgggc
     1201 cgggcaaggt gctttcgggg cttgtcaaga ggatagcccc ggacgctcat gtggttagct
     1261 ctgaggaatt actgagctaa ggacaatcta acattgagga gatacatgat gagggagccg
     1321 ctgatcgtat ccgaccccgc tgtaatgatg ggcaagcctg ttattgcagg tacaagattg
     1381 acagttgaac tcattctgga gaaactggga gcaggcgaaa ccatcgagca attgttggag
     1441 tcgtatccga ggcttacacg cgaagcaatt cttacggctg ctgcatttgg gaaagctcct
     1501 cgagtgagtg gatctaagga gcagtcataa ccggtatgcc aaaggtaatt gctgaagtct
     1561 cattctcgcc catcggcacg ggcacaccca gcctgagccg gtatatcacc gccgccctgg
     1621 ctgttcttga ggcgcacccc gaagtcaggt accaattgaa cccgatgggt accgtgttgg
     1681 agggcgagcg tcgagcggta atcgaggtaa tcgaggctat gaacgaggcg atctttgctg
     1741 ccggtgcgca gcgtgtgggt acgaccctca agatcgacga gcgccgcgac aaggccgcct
     1801 cgatggaaca caaggtcgaa tctgtgatgg aggggcttaa gcgggacggc tagcgcgagc
     1861 ggggttcgcg tgcgtcctgc caacaatcat gttcaagcgg attctcattg ctaacagggg
     1921 agagatagcc ctacgcgtta tccgggcctg ccacgagttg ggcgtggagg cggtggtcgc
     1981 ctattcggag gccgaccgcg agacgctgcc ggtgcgcctg gccgacacgg ctatctgcat
     2041 aggccccgcc gcgccgggac gctcctacct gcacatcccc gccgtcatca gcgccgccga
     2101 gatggccggg tgcgacgcca tccaccccgg ctacggcttc ctctccgaaa acccctacat
     2161 cgcggagatc tgcgagaagg tcggcattac cttcgtgggg ccaagctccg aggcaatcca
     2221 ggcgatggct aacaaggtac aggcacgcaa gctcatggct gaggccgggc tgcccatcat
     2281 tcccggcacc gaagacgctc tgttgaacgc ggatgaagcc catgcagtgg cggaggagat
     2341 cggcttcccg ctgttgatga aggcggtggg aggaggcggc ggccggggca tgcgcgtggt
     2401 caacagcata gacgagcttc tcgaggcgta caacctcgcc aggagcgagg cgtcccttgc
     2461 gttcggcaac cccgacgtgt acctggagcg ttacctctac cacccgcgcc acatcgaggt
     2521 gcaggtgctg gccgaccagt acggccactg catccacctg ggtacccgtg attgctcgct
     2581 acagcggcga caccagaaga tactcgaaga ggcacccgcg cccggcctga ccgaggagca
     2641 aacgcgcaga attggggagg cggcggtgct cggcgcgaag gctatcggct acagcacggt
     2701 gggcacgttc gagttcctgc tggacgagtt gggcgagttc tacttcatcg agatgaacac
     2761 ccgcatccag gtggagcatg gcatcaccga aatggttacg ggcgtggacc tggtgaagtg
     2821 gcagatacgc accgccgccg gggaaccctt gacgctcgag cagaaggata ttacaatcac
     2881 cgggcacgcc attgagtgcc gggtgaacgc cgaggacgtg caacggggct tcttaccggc
     2941 gggtgggccg gtcgagttgt acttgccgcc gggcgggcct ggcatacgcg tcgattcgca
     3001 cctctactcc ggctacacag ccccctctaa ttacgactcc ctgttgggca agatcatggc
     3061 ctggggagag gaccggcagg aggccatcgc tcgcgcccgc cgcgccctca tcgaatgcat
     3121 tatcatcggc ctgcccacca cgataccgtt tcaccagaag atactggacg acccgcgctt
     3181 cgtgcagggc cacatccaca ccggcctggt cactgaatgg ctggccgagc aactagtcga
     3241 gaaccccgcg agcgccggca cgagcacggc gggcagcccc aacgggcggg tggcgccagg
     3301 cagggtacag tagtaacgag agggcatcga gcatgcagga caccaagcta ggcaccgtaa
     3361 ggatagcacc aagcgtgctc gccactatag tgagcctcac ggccagcggc atcaagggcg
     3421 tggcgagtat gagtagcgtc accaggcccc cggcgcgggt gcttcagcgc cccaacatgg
     3481 gcagcgcgcc cggcgtttcc ctgcaggtgc gtaacaacac tatagccgcc gacgtttata
     3541 tcgtggcgga acccgacacc aacctcaagc gcctcgccca gaccatccag acccgcgtcc
     3601 gcgaagccct cgaccagatg gtcggaatgt ccgccgaagt ggtgaacgtc tacatcgagg
     3661 atgtgagcga ctagggagtg ccgagtgccg agtaccgagt aacgagaaaa cgtggtttcc
     3721 agcagattcc cttacgtact actcggcact cggcactcgg cactcggcac tcgttacccc
     3781 catgtcaact gccgaacgac gacaagccag gatcaccgcg ctacagacgc tgtacgaggt
     3841 ggacacgacg gagcacacgg ctgaggaggt catgctacgg ctgttcaatg agcctatgtc
     3901 ggaaagccag cccgaggggg acccgttggt ggaagacgtg cgcagctacg cgcggacgct
     3961 ggtggagggt gcggtggagc accagggcga cctggacgag attatcgcga ggtcggcccc
     4021 caactggccc gtggcgcaga tgtcgagcat agacaagaac atactgaggc ttgcaatttt
     4081 cgagattctg tttgataata gggtgcccct gaaggccgcc ataaacgaag cagtcgagct
     4141 aggcaagcgg tttggtagcg atagctccag ccgctttata aatggggtgc ttggcacggt
     4201 agctgctgag caccagcaac tgcgccgcaa atacaacaag gccgcccccg gcgcgaccgc
     4261 caaccaggaa taagtaagga acaccctaag ccgaatgtct tcgcgacagg aactattcag
     4321 gaggctccgt ccgctgcttg ccgaggccct aggggccgac gaggatgaga ttagcctgga
     4381 caccagcgtc tacgacgacc tcaacgccga cacgatagag ttccacgatg aggtcatccc
     4441 gctgattgag gaggaattcg gggttacgat agacgatgac gaggtacctg atctcactac
     4501 cgtgcgcgag ctggtagcat atttggcaga aaacacctag ccaatcccgg cttgaaagga
     4561 gacatatagg aatggcaaac gctacggaac acctcagaga ggtcgccgtc cagcagtacg
     4621 gagtggacga ggcagaggtc gccaacaagg acgcgaacgc tctcaccgaa ctgctcctgg
     4681 gcaagatcgc tatggacaag tacaaggtca gcgcctcaga gcttgagggc ctcgaccgcc
     4741 gtggggagac tcagttgctc ctccagaaca tggtcagcga cctgctgaag gttgacaaga
     4801 gcaaggtaag ccctgagacg accttctcag acctgggagc cgactccctc gacatggtcg
     4861 agctgctcat gactatcgag gacgtgtgcg agccgttcgg cgagatgaag atccctgagg
     4921 aagacgctaa catcggtacc gtgggcgagg ccgtcgaccg catcgactct tacatcacca
     4981 actacgtctc cgcgagctga gatttggaag ttcgaatttc ggatttcgga tttagtaagt
     5041 atccaacaaa tccgcaattc gaatttctaa tttccaaatt ggttatttgt aaaggggcgc
     5101 ggtgggtcct ataatggact tgccgcgcct tttgcgcgcc cgccggtcgt gtggtcactt
     5161 atcagccacc agataatgag cactttgtcg tcaattcgcc agcgcctccc gttcggtaaa
     5221 cgcaacgggc ggccgccgcg tacctcacgc aggcggcccg acggcagcct cgacgtcgag
     5281 atgcccctga tggagcacct gcgcgagttc agggataggc tcatcaagtc ggcgctcacc
     5341 gtcctgattt tcacctccct gagcttcgtg ttcgtggacc gcgagataga ggtgctcctg
     5401 ggtatggcgg gcggaaggaa tctgatcgct atcacggtca cggagaattt cgtcgcgtac
     5461 gtgaaggtgg cgctgattac ggggatcgga ctctcgatgc cggtgctcgt gtaccagttg
     5521 ttccagtttc tcgcccccgg cctgacgagg aacgagaagc gctggatctt gatcagcctg
     5581 cccggcgtca tggctttctt cgtcatggga gtcgtgttct gctacttcct ggtgcttccg
     5641 agcgcgctgg agttcctgct taacttcggc agcccccaga tcgagataca gccgactatc
     5701 tcagaatact tgggctttgt gaccaacttc atgcttgcac ttggcctggc cttcgagacg
     5761 cctctcatca tattcatcct atccaaagtg ggcgtggcta cgcccaagcg gctcaaaagg
     5821 ttccgccgct gggcctacgt gctggccttc gtagcggcgg ccatcatcac accgacgccc
     5881 gacccggtca accagacgta cgttgccgtc cccattatcc tgctgtacga gcttggcatc
     5941 ctgttcgcat ggctggggag gcccaggacg aagaaggcag cctagcacgt caaccggacc
     6001 tgcctgacga gggtagctag ggcctggcgt ggtgctgcat ccttcttgcc cagcccttta
     6061 gcgtctccgg aagctcgggc acttcaagca acaagctcgc tcctgcgtgg tccttgtgat
     6121 agatgtaagc cacatctccg atagtaacgt tgtgtgtggg ggtgaacacg ctccgcactt
     6181 catcgcccgc gccaatgtcg ccctccctga gtattcgcag gtacacacct ggacgcaggg
     6241 cagcgccgaa cctgggcggg aaatcggggt ctcccatgcg agcgcctagc ttccagcagg
     6301 ggaaccgcgg ctgtgccacc tctaggaggg cggtgccaat ctcccaccgc tccccgatta
     6361 ccgcgccgtt catatctacg cctgcaagcg tcaggttttc accgaacgta cccggttcga
     6421 gcgagcggcc cacctcctgc tcccaccact ccgcgtcctc gcggccgtag gagtagacag
     6481 ccttatcgcg cccgccatgc acccgccggt ctgcctggtc gtcaccctct aggttcaccc
     6541 cgcgcagggg tacgcgcccg gccaccggcg atttccagat ggcggtcgtg tccctctggc
     6601 ccagccactc gatttcgcgc ggcttcccca cgttcaccga aagcactctc cctggcgttg
     6661 tgcttgatac cgggtctgtc attgctggat tctcctggta ttttctgcgt aattagctac
     6721 gatgggcgca tgcaacccca atagatagga ccaaagcctc ccctttgtca ttccgaacgc
     6781 agtcaggaat caggtggccg tcgcgcactg ttgcaatcga aggagaaacg gacgcaaggc
     6841 aggt
//
LOCUS       DASRWZ010000187         4047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_700516, whole genome shotgun sequence.
ACCESSION   DASRWZ010000187 DASRWZ010000000
VERSION     DASRWZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700516"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1472)
                     /gene="nuoH"
                     /locus_tag="VFH60_02865"
     CDS             complement(135..1472)
                     /gene="nuoH"
                     /locus_tag="VFH60_02865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET6312751.1"
                     /translation="MEFWLIVLRAALAFVIVNVGMVLVAFLVYFERKVAAHMQARQGP
                     NRAGPIGLLQSFADLLKMLVKENTVPTKADKIVFFLGPVVATFAALGALSVIPWGPGR
                     NQPGHIDLFGQQIGWPIAEVNVGILLILAMSSLGVYGLIMGGWSSNSKYSLLGGLRSS
                     SQVISYEITLGISLVGVFIMSGTLDLIGITSAQHPLFHNPTQPGIWFILLQPVAFFIF
                     LTSAIAETNRTPFDLPEAESELVGGYHTEYSGIRFGLYFLGEYIGMLTVSAITSVCFL
                     GGFTSPFAAWFDQPAIGAAPAIDLPFISGALGSGLHWLVLKIAVFIFVYYWLRWTLPR
                     FRYDQLMGLCWKVFLPATLANILILSLLKLIIFPPGISVAEYADRWWGWWIIVAIQVV
                     MGILTLLGVSRMAGLSWFGRAERPVLVDRQVILVRNVHGGRGTIEGEARTIKP"
     gene            complement(1518..>4047)
                     /locus_tag="VFH60_02870"
     CDS             complement(1518..>4047)
                     /locus_tag="VFH60_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET6312752.1"
                     /translation="ETPCVSCGQCVELCPVGALSSRLYSSRMTLEHHWWQRKTETTCN
                     SCSVGCTIKLGSTRNQIWEVRSDDALGLNDGRTCVKGRFGQDYINSNSRLSTPLIRRD
                     GKFVMATWDEALDYVASRLNEHKGQVGVVASPKITNEENYALQKVARLGLGTNNIDYN
                     GRSHEAPALEVLREMLGYAAPTNNFLDTRQKAGCVLTIGDSIYETHPVYAYQLQRMIR
                     LMGKKLIVVSPRWTKMCDWSTLWLAPHAGTEELLVNGIARAILDAGLIDQAAVEAKAD
                     NVLAYTEALGGANFALDAVSSATGVEAKDILAAAYLYATGGLDAGPQPLDSRTHPSNL
                     PLMMLAAGGDVASGARNGGTDADVTPQGGGPVNYVVNKPERGFPPSTIVFPAGGTYTL
                     TPRTVAALTNLALATGNVGREGGGVNPLMLDTNCLGANDMGCQPGYFPGYRPVNADNA
                     RVMEELWTANMDRKAPEVPSELGLDLLGMLNAADTGDIKAMWVVGANPVLTTEAWDTT
                     RVRAALEKLDFLVVQDIFMNETGELADVILPASSYAEKEGTFTNAERRVQRVRQSIEP
                     VGVSRPDLAIISSIGERLGVPMPGLDPLNVFAEIVRTVPQYAGMSYSRLDMTEFIDDA
                     IPMPAAVSYKQLKVKSLMWPCTDRLSPGSPVLYTDGFATTTGKARMWTERQQGTMAGS
                     YRSADAQTQAAGGTLLATLGFGLFPFNTGTLSRHSYGLSRVEPTPRLHIHTADAGPLG
                     IVDEMPVLVMADGVEGGESIYAVALVYDRIPQGRAFLAFTLEQYGTNPTVRQLRHQIA
                     SDTTGGRKAVALRVQPAPNRTMDSMREFQPVATANVLDTRIQPI"
BASE COUNT          707 a         1288 c         1316 g          736 t
ORIGIN      
        1 cccgccgatc tggtttacct agtctgccat ttgtagctcc tatgagttat gagtaatgag
       61 tgatgaggac tgagggcagc ggattgaggg atacataagc ttctcagtcc tcagtcctca
      121 tcactcatta ctcgtcacgg ctttatagtt ctcgcttcac cctctattgt gccccggccg
      181 ccgtgcacgt tgcgtacgag gattacctgg cggtcaacca gcacggggcg ctcggccctc
      241 ccgaaccagc taagcccggc catgcgcgac acgcccagga gggtcagtat acccatcacc
      301 acctgtatcg ccacgataat ccaccagccc caccagcggt cggcgtactc cgccaccgag
      361 atgcccggcg ggaatatgat cagcttgagc agggagagta tcaggatgtt cgccaacgtg
      421 gcgggcagga acaccttcca gcacaggccc ataagctggt cgtagcggaa gcgcggcaac
      481 gtccagcgca gccagtagta cacgaagatg aacaccgcga ttttgagcac cagccagtgc
      541 aggcccgagc cgagcgcgcc gctgatgaac ggcaggtcta tcgcgggggc cgcccctatc
      601 gcgggctggt cgaaccacgc ggcgaagggc gaggtaaagc cccccaggaa gcagaccgag
      661 gtaatggccg acacggtgag catgccgatg tactcgccca ggaagtagag gccgaaccgg
      721 atgccgctgt actccgtgtg gtagccgccc acaagttccg actcggcttc tgggaggtcg
      781 aacggggtgc ggttggtctc cgcgatggcc gaggtgagaa agatgaagaa ggccacgggc
      841 tgtagcaaga tgaaccagat acccggctgc gttgggttgt ggaagagcgg gtgctgcgcc
      901 gaggtgatgc ctatcaggtc gagcgtgcct gacatgatga acacacctac gagcgagata
      961 cccagagtga tttcgtagct gatgacctgg gacgacgaac gcaggccgcc cagcaacgag
     1021 tacttgctgt tggaggacca gccgcccatg atgaggccgt acacgccgag ggaggacatc
     1081 gccaggatga gcaggatgcc cacgttcact tcagcgatgg gccaccctat ctgctggccg
     1141 aacaggtcta tgtgccccgg ctggttgcgg cccggacccc acggtatcac cgagagcgcg
     1201 cccagggccg cgaaggtcgc caccacgggg ccgaggaaga acacaatctt gtccgccttg
     1261 gtcggcaccg tgttttcctt cacgagcatc ttgagcaggt cggcaaagct ttgcagcaag
     1321 cctatcggcc ccgccctgtt cggcccttgc cgcgcctgca tgtgtgcggc caccttgcgc
     1381 tcgaagtaca caaggaaagc caccagcacc atgcccacgt tcacgatgac gaaggcaagt
     1441 gcggccctga gcactatcaa ccaaaattcc atatcgtttc cctctaggag gtaagaagcg
     1501 cgttggcgct tcctacgtca aattggctgt atcctcgtat cgagcacgtt agcggtagcc
     1561 accggctgga actcccgcat gctgtccatc gtgcggttgg gtgcgggctg cacccggagc
     1621 gccaccgcct tgcggccccc cgtggtgtcg ctggctatct ggtgccgcaa ctgccgcacg
     1681 gtcgggttgg tgccgtactg ttccagcgtg aaggcgagga aggcgcggcc ctgcggtatg
     1741 cggtcgtaca ccagcgccac cgcgtaaatc gattcgcccc cctccacacc gtccgccatc
     1801 accagcaccg gcatctcatc cacgatgccc agtggccccg cgtccgccgt gtggatgtga
     1861 aggcgcgggg taggctcgac gcggctgaga ccgtagctgt ggcggctgag cgtgcccgta
     1921 ttgaacggga ataggccgaa ccccagcgtg gcaaggagcg tgcccccggc ggcctgagtc
     1981 tgagcgtcag ccgaacggta cgagcctgcc atcgtgccct gttgccgctc cgtccacatc
     2041 cgcgccttgc cggtagtggt agcaaagccg tccgtgtaga gcacagggct gccggggcta
     2101 aggcggtcgg tgcacggcca catgaggctc ttcaccttga gctgcttgta agagacggcg
     2161 gcgggcatcg gtatcgcgtc gtctatgaac tcggtcatgt cgaggcgcga gtagctcatg
     2221 cccgcgtact gcggcacggt gcgcacgatc tcggcaaaga cgttcagcgg gtcgaggccg
     2281 ggcatcggca cgcccagccg ctcgcctatg ctgctgatga tcgccaggtc gggcctgctt
     2341 acgcccaccg gctcgatgga ctggcgcacc cgctgcaccc ggcgctcggc gttggtgaag
     2401 gtgccttcct tctcagcgta ggacgaggcg ggcagtatca cgtcggcaag ctcgcccgtc
     2461 tcgttcatga aaatgtcctg caccaccagg aaatcgagct tttccagcgc cgcgcgcact
     2521 ctggtggtgt cccacgcctc ggtcgtcagc accgggttcg cgcccaccac ccacatcgcc
     2581 ttgatgtcgc ccgtatcggc ggcgttgagc atgcccagca ggtcgaggcc cagctcggag
     2641 ggcacttcag gggccttgcg gtccatgttg gccgtccaaa gctcctccat cacgcgggcg
     2701 ttgtcggcgt tcacggggcg gtagcctggg aagtagcccg gctggcagcc catgtcgttc
     2761 gcgcccaggc agttagtgtc gagcatcagc gggttgacgc ccccgccctc gcgccccacg
     2821 ttgcccgtcg cgagggccag gttggtgagg gccgccacgg tgcgcggggt gagggtatac
     2881 gtcccgcccg ccgggaagac aatcgtggag ggcgggaagc cgcgttccgg cttgttcacc
     2941 acgtagttga ccggcccgcc tccctgcgga gtaacgtcgg cgtccgtgcc gccgttgcgc
     3001 gcgccgctcg ccacgtcccc acccgcggcg agcatcatca ggggcaggtt gctggggtgg
     3061 gtgcggctat ccaggggctg cggacctgca tcgaggccgc cggtggcgta caggtaggcg
     3121 gcggccagta tgtccttggc ctccactccc gtggcgctgc ttactgcgtc cagcgcgaag
     3181 ttggccccgc caagcgcctc cgtgtaagcg agcacgttgt cggccttggc ctccacagcc
     3241 gcctggtcta ttagcccagc atccaggatg gcgcgggcga tgccgttcac cagcagctcc
     3301 tcggtgcccg cgtgcggtgc cagccagagg gtagaccagt cgcacatctt cgtccagcgg
     3361 gggctgacca cgatgagctt cttgcccatg aggcgaatca tgcgctgtag ctggtaagcg
     3421 tacaccgggt gggtctcgta gatgctgtcg ccaatcgtga gcacgcaacc ggctttctgg
     3481 cgcgtgtcga ggaagttgtt ggtaggcgcg gcgtagccca gcatctcgcg gagcacttcc
     3541 aacgcggggg cctcgtggct gcggccgttg tagtcgatat tgttggtacc gaggcccagc
     3601 cgggccacct tctgcaaggc gtagttctct tcgttggtga tcttggggga ggcgaccacg
     3661 ccaacctgtc ctttatgctc gttcaggcgg gaggcaacgt aatcgagcgc ctcgtcccag
     3721 gtagccatga cgaacttacc gtcgcggcgg atgagggggg tgctcaggcg gctgttggag
     3781 ttgatgtagt cctgcccgaa gcggcccttg acgcaggtgc gtccgtcgtt gaggccgagc
     3841 gcatcgtccg agcgcacttc ccagatctgg ttgcgggtgg agcctagctt gatcgtgcaa
     3901 cccacggagc aggagttgca ggtcgtctcg gtcttgcgct gccaccagtg gtgctccaac
     3961 gtcatcctgc tgctgtagag gcggctggag agagcaccta ccgggcaaag ctccacacac
     4021 tgcccgcacg acacgcaggg cgtctca
//
LOCUS       DASRWZ010000188         4919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_15418,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000188 DASRWZ010000000
VERSION     DASRWZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15418"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..1860
                     /locus_tag="VFH60_02875"
     CDS             409..1860
                     /locus_tag="VFH60_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HET6312753.1"
                     /translation="MNRHYESIISGRTSRIARAVMLMLLAGVSTLALAVPVRAGATMP
                     DLGTAGSFGVLGGSAVTNTGPTTVDGDVGVSPGTSVTGFPPGLATGTIHAGDAVAAGA
                     QADANTAYNDAAGQACDFDLTGQDLGGLTLTPGVYCFSSSAQLTGRLTLDGGPGSVFV
                     FRIGSTLTTASNAEVSVINGVEPCNVFWQVGSSATLGTNTDFQGNILALTSITLNTGT
                     STIGGLYALNGAVTLDTNDIQACEIAGPPSTPTTAGTAASTATLTALPATGTSTAATN
                     TPVPAATNTTVPTATDTPVPVATDTPGPRNTRTRSDTPTPEPEDTEVATEVATEVATS
                     VATDTPSDTALATTTPSDTSTTTPTLAATNTPTNTITLSTPTVMLTGTPLSTETPGGP
                     SVTPTAPIPGDTPTEAPPAATLTETPVAPGGTPEAAVSAVPPPPLLLPPTGGSANLGL
                     PWLDSVVPLLIALLLAVGVRLKMRSRSRVRHKI"
     gene            2340..4877
                     /locus_tag="VFH60_02880"
     CDS             2340..4877
                     /locus_tag="VFH60_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET6312754.1"
                     /translation="MAEVNYLNFDVVIQRSGEGYRAQVQSPAGEASSHFTNPFSQIEV
                     EHFMLSAAPGKRNTRRIDAPEVGAAKKFGEKLYNSVFTGDVGAVLRSSLDEAWQRNSK
                     LRVRLRLTDVPELADIPWEYLYNPSLNRFFALDDDTAIVRYLDVPERVRPVTVTLPLR
                     VLVMISAPTDYAQLDVQQEWANLNEALADLERNGIVELQLLEDASLSALRKQLTKGQY
                     HIFHFIGHGGFDRQTEEGVLVMRDEAGRGRFVSGQQLGWLLHNHRTLSLAILNACEGA
                     KADRADAFAGTGQSLVQQGIPAVIAMQFEITDEAAKTFAYGFYSALALGQPVETALTE
                     TRLTLFSEDHGLEWGTPVLYMRSTDGRVFNVQQQEEAARKQTRITELYQAAQAAMATH
                     DWTAATASWQAILAIDPGHVHAAAGLEQVGKQQELAALYDQGRSHLGAGRWQEAVTAL
                     GRAQAIDANYRDVSALLGDARQRLDAVQRSQAGSAPHTPMHGTPAVDLRGAQPYAGPP
                     QGAQGAGAAGIGQQTGMQAISQQQVVGPQPVVMPGPYTGGGVPGPQTQGAGGGSSTGG
                     RGAYEGPVPAPVPAPVPRRRVAWVVPAVLLVVLAVFGTAAFAIISSQQAGERAAQAAS
                     TATVEKQAANTRATRTAVARNRARANATGTAEALEAIISANATSTAYANLLEVSAQST
                     ATAQAEATEAAGTITDPTARSAHIESVTLEHNLYNAEGVKGMEINVVFTTENLKDVVL
                     PVNVYFSWPDGTKIVSMDGTYQAPDNQVGVSKSAQPGYVSSRYDDFKIFMPYSELEMD
                     FRADTFDIQLQVIIFDDKSVELTRSAYIPFSYTSPGPVTEPTRITIP"
BASE COUNT         1004 a         1587 c         1515 g          813 t
ORIGIN      
        1 actactccgc ctggctgcgg tgacacggtg ctatgctgag agcataatcg catcgggcga
       61 cttagcagca ggggcatcag cgaataagac agtttgaccc ctactctgcg gcgcattttc
      121 tagcgagcgc gcgcacgaag cttgctggcc ttgccacgct aggactttag tccagccagg
      181 atcgtctgcg cataacccaa aagtactgcc tctctgacgc ctttcaggac cgctgggcta
      241 ttgtcgcaac ggccgcttgc gggtacgatg tagaagcctg gggcgttggg cgcaccagga
      301 atgacctaac aacacactca ggactttcga cttcagaagc gaatcttcgg agcgaaagtc
      361 tttttgcttt aagcccaggt cattactaga gggaggcgca ggcggaatat gaatcgacac
      421 tatgaatcta tcatcagcgg caggacatct cgaatagcgc gagcggttat gcttatgctg
      481 ttggcggggg tctcgacgct cgcactggcc gtgccggtac gagccggggc gactatgccc
      541 gacctgggaa cggcgggcag tttcggcgtg ctgggcggca gcgcggtcac caataccggc
      601 ccgaccacgg tagacggcga tgtcggagta agccccggga cttcggtgac cgggttcccg
      661 cccggcctcg ccaccgggac catacacgcg ggcgatgcgg ttgcggcagg ggcgcaggca
      721 gacgcgaaca ccgcctataa cgatgctgcc gggcaagcct gcgatttcga cctgacaggc
      781 caggacctgg gcggcctgac gctcactccg ggcgtgtact gtttttcctc ctccgcccag
      841 ttgaccggcc ggctcaccct ggatggcggc cccggtagcg tgttcgtttt tcggataggc
      901 agcaccctca cgaccgccag caatgccgag gtatcggtta tcaacggcgt ggagccatgc
      961 aacgtcttct ggcaggtcgg tagctccgcg acgttgggga caaacacaga cttccagggg
     1021 aacatcctgg ctctgacttc catcaccctc aatacgggca catcaaccat cggtggcctc
     1081 tatgctctga atggcgctgt gacattggac accaacgaca ttcaagcctg cgaaatagca
     1141 ggcccaccaa gcacgccaac caccgccggc accgctgctt ctaccgccac tctgacggcg
     1201 ctacccgcca ccggcacgtc tacagccgca actaatacgc cggtgccggc agctaccaat
     1261 accaccgtcc ctaccgcgac tgacacgccg gtacctgttg cgactgacac gccggggcct
     1321 cgcaatactc gcacgcggtc cgatacgcct acgccggagc cggaagatac agaggtagcc
     1381 acagaagtag ccacagaagt agccacaagt gtagctaccg acacgccgtc agatacagcc
     1441 ctggccacta ccacgccgtc agatacgagt acgaccaccc ccactcttgc cgctaccaat
     1501 acccctacca acacgatcac actgtctacg ccaaccgtga tgctaaccgg tacgccgcta
     1561 tctacggaga cacccggagg gccgagcgtc accccgaccg cccccatccc cggcgatacg
     1621 ccaaccgagg cacccccggc agcgacgttg accgagacgc cggttgcgcc gggcggcacc
     1681 cctgaagcgg cagtctcggc ggtgccaccc ccaccgttgc tcctgccgcc caccggcggg
     1741 tccgccaacc tcggcctccc ctggctcgac tctgtggtcc cgctcctcat tgccttacta
     1801 ctggcggtgg gcgtaaggtt aaagatgcgt agccggtccc gcgtgaggca caaaatctag
     1861 ggaatatcca actgcaaaga agtacagggg cgtgctgctg gcacgcccca acgagtgcaa
     1921 agctctactt ttcgccgccc accccgccag ggctgtgtca aagtcgaatg tagagggtaa
     1981 agatggcctt tgtcattctg agcgcagcga agaatcacgt cccagaccga gatggttcat
     2041 ctttcttatg gagacagtgg agacagtgga gacagtacag agagtggggt taagaaaggg
     2101 gctacttggg tgctgtagga cgagattctt cgctgcgctc agaatgacaa agcccgacac
     2161 actgctgctc gcctactttg acaaagccct agccaccacg cgggcaagta ttgacatcgg
     2221 gccctttccc gatttatagt gctattatag ttagcatatc ttcagagcag cttcgcccac
     2281 gaggagcctg ctcttgttgc cgttaggcgt ctcctttgga ggacaaaagg cggggcatca
     2341 tggcggaagt aaattatctc aacttcgacg tggtaatcca gcgctcaggc gaaggctacc
     2401 gggcgcaggt gcagtcgccc gcgggggagg ccagcagcca cttcaccaac cccttctcgc
     2461 agatagaggt ggagcacttc atgttgagtg cggcccccgg caagcgcaac acgcgcagga
     2521 tcgacgcgcc ggaggtgggc gcggccaaga agttcggcga gaagctctac aactcggtat
     2581 tcaccggcga cgttggagcg gtgctgcgca gcagcctgga cgaagcctgg cagcgtaact
     2641 ccaagctgcg ggtgcgcctg cgcctcaccg acgtgccaga actggccgac atcccgtggg
     2701 agtacctgta caacccctcg ctcaaccgct tcttcgccct ggacgacgat acggctatcg
     2761 tgcgctacct ggacgtgccg gagcgcgtgc ggccagtgac ggtgacgctg cccctgcgcg
     2821 tgctggtgat gatttcggca cccaccgact acgcgcaact cgacgtgcag caggagtggg
     2881 ccaacctgaa cgaggccctg gcagacctgg agcgcaacgg cattgtagag cttcagttgt
     2941 tggaagacgc ctcgctctcc gcgttgcgca agcaactgac gaagggacag taccacatct
     3001 tccacttcat cgggcacggc ggcttcgaca ggcagaccga ggaaggcgtg ctcgtgatgc
     3061 gagacgaggc gggccggggc cggttcgtga gcggccaaca actgggctgg ctgctgcaca
     3121 accaccgcac cctctcgctc gccatcctca acgcgtgcga gggggccaag gccgaccgcg
     3181 ccgacgcctt cgcgggcacc gggcagagcc tcgtgcagca gggcatcccc gccgtgatcg
     3241 ccatgcagtt cgagattacg gacgaggccg ccaagacgtt cgcctacggc ttctacagcg
     3301 ccctcgcgct cggacagccg gtggagacgg cgctcaccga gacccgcctg accctcttct
     3361 cggaggacca cggcctcgaa tggggcacgc cggtgctcta catgcgctct accgacgggc
     3421 gcgtgttcaa cgtgcagcag caagaggagg cggccaggaa gcagacgcgt ataacggagc
     3481 tttaccaggc ggcgcaggcc gcaatggcga cccacgactg gacggcggcc acggcaagct
     3541 ggcaggctat cctggctata gaccccggcc acgtacacgc tgccgccggg ttggagcagg
     3601 ttggcaagca gcaagaactg gccgcgctgt acgaccaggg ccgctcgcac ctgggggcgg
     3661 gacgctggca ggaggccgtc accgcgcttg gcagggcgca agcgatagac gccaactaca
     3721 gagacgtaag cgccctcctc ggcgatgccc ggcagaggct cgatgcagtc cagaggagcc
     3781 aggcaggcag cgcaccccac acgcccatgc acggcacgcc cgctgtagac cttcgcgggg
     3841 cgcagccgta cgcggggccg ccacagggtg cccagggtgc cggtgccgcc gggattgggc
     3901 aacagaccgg catgcaggcg atatcgcaac aacaggtagt tggcccgcag cctgtcgtga
     3961 tgcccggccc ttacaccggc ggcggtgtgc ctggacctca gacgcaaggt gccggtggcg
     4021 ggagtagtac cggtgggcgg ggtgcgtacg aaggaccggt cccggcaccc gtacccgcgc
     4081 ccgtgcccag gcgcagggtg gcgtgggtgg taccggcggt gctgttggta gtgctggcgg
     4141 tgttcggcac ggcggcgttc gcgataatat catcgcagca ggcgggggag agggcggccc
     4201 aggccgcgtc taccgccacc gtcgagaagc aggcagccaa cacaagggcc accaggacgg
     4261 cggtcgccag gaaccgcgcc agggccaacg ccaccggtac cgccgaggca ctcgaggcca
     4321 tcattagcgc aaacgctacc tccacggcct acgccaacct gttagaggta agcgcccaga
     4381 gcaccgccac cgcgcaggct gaggctacgg aagcggccgg cacgataacc gaccctaccg
     4441 cgcgcagcgc tcacatcgag tcggtgacgt tggagcataa cctgtacaat gccgagggtg
     4501 tcaagggcat ggagatcaac gtcgtgttta cgaccgagaa cctgaaggac gtggtattgc
     4561 cggtaaacgt ctacttctcc tggcccgacg gcacgaagat cgtaagcatg gacggcacct
     4621 accaggcacc cgacaaccag gtaggggtga gcaaaagcgc gcaacccggc tacgtctcgt
     4681 cccgctacga cgacttcaag atattcatgc cctactctga gcttgagatg gacttcaggg
     4741 cggacacgtt cgatatacaa ttgcaggtga tcatcttcga cgacaagtct gtcgaactga
     4801 cacgctcggc ctatattccc ttcagctaca caagccccgg cccggtgacg gagcctaccc
     4861 gcatcaccat cccgtaaggt cgtcgttttt ttgtagtgct gttattcggg gcagcgata
//
LOCUS       DASRWZ010000189         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_289772, whole genome shotgun sequence.
ACCESSION   DASRWZ010000189 DASRWZ010000000
VERSION     DASRWZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289772"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="VFH60_02885"
     CDS             <1..1562
                     /locus_tag="VFH60_02885"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HET6312755.1"
                     /translation="AVVPLVQRVLVWDALTADRRYLFIPHDLLKGNTSGAPSEIAHRM
                     QPKLDAPAPHTVESRLPYEMAWDMLTLASSAARQELSLTLQEGRITKRVAKKLNDELL
                     HPTDIKAGTDYIDVLVHLAHALGLLVEREGEQPAYAATPKAEDWAKLSFDAHRRRIFG
                     FWQDDRKWVEPAAYGTIYWWNSDLTGARKRLVKHIAALPVGQWISLEGFLRKVHLNEP
                     FIIWSQEELVRRFGLRALQGFRSQWFEIEGRIITDMLKTMLYWLGAVDLGRDKPKRLI
                     SFRLTPEGAALLDPEVAEHPTTSQRTTHGAPEKTLMVQPNFEVLVLHPDSHVVWNLLR
                     VADLVRHDRVSVYTLNKESVLRAIEHGMSPGEIQQFLQANTGKELPQNVFHSIADWAR
                     LVRRLDVVRATLIEVDEPTLLDELMASRKTRRYITRRLSPTTAIATLPATSDSALSDP
                     WQRLIKELRGAGYIPRYVEEIMSTDEPEGKPTNSHDQPTSLPAKRATGRTPSTTGRRR
                     AATEHSKTGTQ"
     gene            1565..2143
                     /gene="ruvA"
                     /locus_tag="VFH60_02890"
     CDS             1565..2143
                     /gene="ruvA"
                     /locus_tag="VFH60_02890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET6312756.1"
                     /translation="MIVGLEGTVASFGVDHIVLNVGGVFYKVAVPASSISQLGKKGET
                     VRVYTHLYVREDQLALYGAADERQLRMFETLLGVSGIGPKVGLSILSTMPVESLENAI
                     ASGNVDLLTRIPGIGRKTASRLVLELKGKLDLLVAAGISASPTAASEVVEALSGLGYS
                     PAEIQAALSTLPKEQELTTEDMVMFALKRLGR"
     gene            2161..>2700
                     /locus_tag="VFH60_02895"
     CDS             2161..>2700
                     /locus_tag="VFH60_02895"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET6312757.1"
                     /translation="MNHLILASGSPRRHELLARLTPDFTILTSPVEEVGSTLMPEWHL
                     ESVELPLPYHIPEEHDPRLWAWRKAMDVLNTNATPANTLILGADTVVVGLSDLLGKPR
                     DVAHARTILETLRGIEHFVVTGLVVLQAKGDLTADTIQRGATVTRVIMGDYPDAAIEA
                     YIATGEPMDKAGAYALQGLG"
BASE COUNT          566 a          930 c          768 g          436 t
ORIGIN      
        1 ccgccgtcgt acccctggtg cagcgcgtcc tcgtgtggga tgccctcacc gccgacaggc
       61 gttacctctt catcccgcac gacctcttga agggcaacac gtcgggagcg ccctccgaga
      121 tagcccaccg catgcagccc aagctcgacg cgcccgcacc acacaccgta gagagccgcc
      181 tgccttacga gatggcctgg gacatgctca cgctcgcatc gtcagccgcc cgccaggagt
      241 tgagcctcac cttgcaggag ggacgcatca ccaagcgcgt cgccaagaag ctgaacgacg
      301 agcttttgca ccccaccgac atcaaggcgg gcaccgacta cattgacgtg ctggtacacc
      361 tcgcccacgc gctgggcctg ctggtagaga gagaggggga gcagcccgcc tacgccgcca
      421 cgccgaaagc tgaagattgg gcaaagctgt cgttcgacgc gcaccggagg cgcatcttcg
      481 gcttctggca ggatgaccgc aagtgggtcg agcctgcggc ctacggcacc atctactggt
      541 ggaacagcga cctcaccggc gcgcgcaagc ggctcgtcaa gcacatagcc gcgctccccg
      601 tggggcaatg gatcagcctc gaaggcttcc ttcgcaaagt ccacctcaac gagccattca
      661 taatctggag ccaggaggag cttgtgcgcc gtttcgggtt gcgcgctcta cagggcttcc
      721 gcagccagtg gttcgagatc gagggccgca ttatcacgga catgctcaag acgatgctct
      781 actggttggg cgcggtggac ctgggcaggg acaagcccaa gcgccttatc tccttccgcc
      841 tcacgccgga gggcgcggca ttgctcgacc cggaagttgc cgagcaccca accacctcac
      901 agcgcaccac gcacggcgca cccgaaaaga ccctgatggt gcagcccaac ttcgaagtgt
      961 tggtgctcca ccccgacagc catgttgtgt ggaacctgct gcgcgtggcc gacctcgtgc
     1021 gccacgaccg cgtaagcgtc tacaccctga acaaagagag cgtgctgagg gctatcgagc
     1081 acggcatgtc gccgggggag attcagcagt tcctacaggc gaacaccggc aaagaattgc
     1141 cgcagaacgt cttccactct atagcagact gggcgcggct ggtccgcagg ctggacgtgg
     1201 tgcgcgccac cctaatcgag gtagacgagc ctaccctgct ggatgagctt atggcctcgc
     1261 gcaagacccg ccgctacatc acccgccgcc tatcgcccac caccgcaatc gccaccctcc
     1321 ccgctacgag cgactctgct ctcagtgacc cctggcagcg tctcatcaag gagttgaggg
     1381 gcgcgggcta catcccccgc tacgttgaag agattatgtc aaccgacgag ccggagggca
     1441 agcccaccaa ttcccacgac cagcccacca gtctccccgc caagcgagcc accgggcgca
     1501 ctccctccac gactggcagg cgtcgggccg ctacagagca ctctaagaca gggacgcagt
     1561 aaatatgatc gtgggtctcg aaggcacagt cgccagcttc ggcgtggacc acatagtgct
     1621 gaacgtcggc ggcgtctttt acaaagtagc cgtacccgcc tcatcaatca gccagttggg
     1681 aaagaaaggc gagacggtgc gcgtctacac ccacctctac gtgcgtgagg accagctggc
     1741 cctctacggc gcagccgacg agcggcagtt acgcatgttc gagaccctgc ttggcgtgtc
     1801 cggtatcggt ccaaaggtcg gcctctccat cctgagcacc atgcctgtgg agagcctgga
     1861 gaacgccata gcgagcggca acgtagacct gctgacgcgc atacccggca taggccgcaa
     1921 gaccgcctcg cgcctcgtgc tcgaactcaa aggcaagctc gaccttctcg tggccgcagg
     1981 tatctccgcc agccccaccg ccgccagcga agtcgtagag gccctttccg gcctcggcta
     2041 cagccccgcc gagatacagg ctgcgctctc caccctcccc aaagagcagg aactgaccac
     2101 cgaagacatg gtcatgttcg ccctaaaacg gctgggccgc tagctcccga caggcgcgga
     2161 atgaatcatc taatactcgc ctccggctcc ccacgccgcc acgaactcct tgcccgcctg
     2221 acccccgact ttaccatcct gaccagcccc gtcgaggagg ttggatcaac cttgatgccg
     2281 gagtggcatc tcgaatctgt agagctaccc ctcccctatc acatccccga agagcacgac
     2341 ccccgcctat gggcctggcg caaagcgatg gacgtgctca acacgaacgc cacgcccgcc
     2401 aacactctca tactaggcgc ggatacagtg gtagtcggcc tgtctgacct cctgggcaaa
     2461 ccgcgagacg tcgcccatgc gcggactatc ctcgaaactc tgaggggaat agagcacttc
     2521 gtcgtgaccg gccttgtagt gctacaagct aagggcgacc tcacagcaga cacgatccaa
     2581 cggggcgcaa cggttacgag ggtaataatg ggtgactacc cggatgctgc catagaggca
     2641 tatattgcca ctggcgaacc tatggacaaa gcaggggcct acgccctaca agggctagga
//
LOCUS       DASRWZ010000190         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1249063, whole genome shotgun sequence.
ACCESSION   DASRWZ010000190 DASRWZ010000000
VERSION     DASRWZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249063"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1246
                     /locus_tag="VFH60_02900"
     CDS             200..1246
                     /locus_tag="VFH60_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6312758.1"
                     /translation="MSKSKSKSKIPLNDGSLALSGAYEVEHEAERDTSPLRSGRLKLV
                     PRPEPEPETATPDSLYNPSIDTTPRLREHMVPPVSPGAPITDKLDDAPLSAHPSGIGE
                     HAAAVRMVPTVGARVRTGLRRSTLLALLAVAVAGALVASGYLLATTPWLANLTGSAPP
                     AAVSEAPPSGPTGDSAFAQPPGQTQAKDDQLLLSDLPAPQPPSTAAKATRDQALEAYK
                     QGDYAQAVQLFEGYVLVSGDDAEAYYHLGMSYMALAGQEHSMADSELAFRTAISLQPT
                     WAAPYQGLAENLMRRGSYEEAIAPAQKAAELAPAATEVWLTLGRAYQGAGRDFEAARA
                     FSQASYLAPAPPLP"
     gene            1567..1917
                     /locus_tag="VFH60_02905"
     CDS             1567..1917
                     /locus_tag="VFH60_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6312759.1"
                     /translation="MIGPVVRQLRQERNLSQETLANAAHVSSGYLSKLERGLYKSPSF
                     EVLSRIAGALGLQTAELYRAAGIEHPIESSDPKLEPLMETFNPKLADLPKRVRDLIMS
                     EIRRVFREEEEAAT"
     gene            1914..2387
                     /locus_tag="VFH60_02910"
     CDS             1914..2387
                     /locus_tag="VFH60_02910"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HET6312760.1"
                     /translation="MSTLTDEVLDSIDFLVHRYHLHSRVPVDLSPILERFEVKKHNFT
                     PMTMGFVLVRPKQIHIGISKNLDAAWHRMALAHEAAHIIANQPHQLFVCKTGDWSREN
                     DEREAQLIAACLLVPLKVIEDRYRVDVAPYLGRQLKVPADLVDLRWSFARVHGDI"
     gene            2393..>2587
                     /locus_tag="VFH60_02915"
     CDS             2393..>2587
                     /locus_tag="VFH60_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6312761.1"
                     /translation="MPGPPAPIRYRKAIAYITREVEGRKQLLVFTHRDNPEAGVQVPA
                     GTAHEGEDIEATLFREVEEET"
BASE COUNT          486 a          864 c          794 g          443 t
ORIGIN      
        1 tccttcgctg cgctcaggat gacaaggtcc tctttggtcc tgctgttctt tattggagta
       61 gacgtggcat attgaaggtc gtgcgcctcc tgtgatagtg caaggtgtgg aaacgggggg
      121 cagcggagtc gtgcggtgct ataatgccca gctatagaca cgcaatttcc gcgcactacg
      181 cacgggtgag gtaagacaca tgagcaagag caaaagcaag agtaagatac cgctgaacga
      241 cggcagcctg gcgctcagcg gcgcgtacga ggtcgagcac gaggcagagc gggatactag
      301 tcctctgcgg agcggcaggc taaagctggt gccacgtcct gagccggagc ctgagaccgc
      361 cactcccgac agcctgtaca acccctctat cgacaccacc ccccggctca gggagcacat
      421 ggtcccgccc gtgtcgcccg gcgcgccgat aacggacaag ctggacgacg cacccctttc
      481 ggcgcacccc tccggcatag gggagcacgc ggccgccgtg cggatggtgc ccaccgtggg
      541 ggcgcgggtg aggacgggcc tgcgccggag caccttgctg gcgctgcttg ccgtggcggt
      601 agccggtgcc ctggtagcaa gcggctacct gctggctacg accccgtggc tggccaacct
      661 caccggcagc gccccaccgg ccgccgtgag cgaggcccca ccgtctggcc ctaccggcga
      721 tagcgccttc gcgcagccgc cgggacagac ccaggccaaa gacgaccagc tactgctcag
      781 cgacctcccg gccccgcaac ccccctccac cgcggccaaa gctacgaggg accaggcgct
      841 cgaagcctac aagcagggcg actacgccca ggcggtgcag ttgttcgagg gctacgtgct
      901 ggtgagcggc gacgatgccg aggcctacta ccacctgggc atgtcgtaca tggcgctagc
      961 cggacaggag cactcgatgg ccgattcgga gctggccttc cgcaccgcca tttcattgca
     1021 gcccacctgg gccgccccct accagggcct tgccgagaac ctgatgcgcc gaggctccta
     1081 cgaagaagcc atcgcccccg cgcagaaggc agccgagctt gcccccgccg ccaccgaagt
     1141 gtggctcacg ctgggacgag cctaccaggg cgcgggccgc gacttcgagg ctgcgcgggc
     1201 gttctcccag gcatcctacc tcgccccggc accgcccctg ccctgacata tctaaacgcc
     1261 aagacgccaa ggacgcgcca agattaggat tgtagggttc taagctaaag gaacagggta
     1321 aggcattacc taccctgttc cttctttgta ctcactaaac aatcctaaac tttgcgcgtc
     1381 ctttgcggtc tttgcgtctt tgcgtttccg tactcttggc gtggtccctg gcgtcttggc
     1441 gtttggtccc gtcttactgc cgccatcctg gtgggttttg tggtataata cgggactttt
     1501 cgcccggtgg gacgcgttgc tcatattcag ggcagccttc cctgtgccgt ttgcaccgcg
     1561 agccttatga taggtcccgt cgttcgacaa ctcaggcagg agcgcaacct gagccaggag
     1621 acgctcgcca acgccgccca cgtgagcagt ggctacctct ccaagctcga aaggggactc
     1681 tataagtcgc cttccttcga ggtgctcagc cgcatagccg gggcgttggg gctacaaacg
     1741 gcggagcttt accgcgccgc cggtatcgag cacccgatag agtccagcga ccccaagctg
     1801 gagccgctga tggagacgtt caaccccaag ctggccgacc tgcccaagcg cgtccgcgac
     1861 ctcatcatgt ccgaaatccg ccgggtgttc cgcgaggaag aggaggctgc cacgtgagca
     1921 cccttaccga cgaagtgctc gactcgattg acttcctggt ccaccgctac cacctgcact
     1981 cccgcgtgcc cgtggacctc agccccatat tggagcggtt cgaggtcaag aagcacaatt
     2041 tcacgcctat gacgatgggg ttcgtgctgg tgcggcccaa gcagatccac atcggtatca
     2101 gcaagaacct cgacgcggcc tggcaccgta tggcgctggc ccatgaagcg gcccacatca
     2161 tcgccaacca gccccaccag cttttcgtgt gcaagacggg cgactggtcc cgcgaaaacg
     2221 acgagcggga ggcccagttg atcgcggcct gcctgctggt gccgctgaag gtgatagagg
     2281 accgctaccg cgtggacgtc gccccttatc tcggcagaca actcaaggtg cccgccgacc
     2341 tggtagatct gcgctggtcg ttcgcccgcg tgcatggaga catataggcg tgctgcccgg
     2401 cccgcccgca ccaatcaggt accggaaagc tatagcctac atcacgcgag aagtcgaggg
     2461 ccgcaagcag ttgctcgtct tcacccaccg ggacaacccg gaggcgggcg tgcaggtccc
     2521 ggcgggtacc gcgcacgagg gtgaggacat cgaggctacc ctcttccgcg aggtggagga
     2581 ggagacc
//
LOCUS       DASRWZ010000191         1841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1043472, whole genome shotgun sequence.
ACCESSION   DASRWZ010000191 DASRWZ010000000
VERSION     DASRWZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043472"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VFH60_02920"
     CDS             complement(<1..883)
                     /locus_tag="VFH60_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET6312762.1"
                     /translation="MFRTLRSKLLWSFAAVSVLMMVLVLAVGFVLARNNARNAAFANL
                     EQKRAIAVPYIALVLLDERPQGGRPAVTEAARHALDVAGLRVFLLDRDTLSVEHDTGD
                     NAALLAKKFPFYLAADLEDQLRSGRPVRGTTNFEGESRQYQYIAQLTPLPARPPGGLP
                     GRASRQFVVVAQPEPDLARVLQNAQESLLPAVLAGLAASLVVAYFLARSISGPVSKLA
                     EAAAAVSRGDYGQRLPVQGHDEIATLTHRFNVMAEEVGNTHQAQRDFVANVSHDLKTP
                     LTSIQGVSQAMLDGSIRE"
     gene            complement(903..1607)
                     /locus_tag="VFH60_02925"
     CDS             complement(903..1607)
                     /locus_tag="VFH60_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6312763.1"
                     /translation="MSDKTILVVDNEPNIIELVKLYLGNEGFRVEQANNGRQALERYR
                     ACAPALVVLDLMVPEVDGWEVCRQIRREGDTPIIMLTARSDDVDKIVGLELGADDYLT
                     KPFNPRELVARVKAVLRRTAARHSAGQTGQPLQFGNTTVDEARHEVTVGGKRVELRAK
                     EFELLLAFVQHIGLVLERDKLLNVVWGYDYFGDTRTVDVHVAHLRDRLQGSSLTLQTI
                     RGVGYKMVLQAAETPA"
BASE COUNT          330 a          574 c          589 g          348 t
ORIGIN      
        1 cctcacgtat cgagccgtcc agcatcgcct gggagacgcc ctgtatggag gtgagagggg
       61 tcttgaggtc gtgggagacg ttggcaacga aatcgcgctg tgcctggtgg gtgttgccta
      121 cctcctccgc catgacgttg aaccggtgag ttagcgtggc aatctcgtcg tgcccttgca
      181 ccggtaggcg ctgcccgtag tcgcccctgg acacggcagc cgccgcctcg gcaagcttgc
      241 tgacggggcc ggagatggaa cgcgccagga agtaagcgac cacgagggag gccgcaagcc
      301 cggccagcac ggcggggagc agcgactctt gcgcgttttg tagcacgcgg gcgaggtccg
      361 gttcgggctg ggccaccacc acgaactgcc gggaggcccg tccgggcagc cctccgggcg
      421 ggcgggccgg aagcggtgtt agctgggcta tgtactggta ctgccgggac tcgccctcga
      481 aattagtagt cccccttacg ggtctgccgc tcctgagttg gtcctcgagg tcggcagcaa
      541 ggtagaaggg gaacttcttt gcgagcagcg ccgcgttgtc gccggtgtcg tgctccaccg
      601 agagcgtgtc acggtcgagc aagaacacac ggaggcccgc cacatcgagc gcgtggcggg
      661 cggcctcggt gacggcaggt ctaccccctt gcggccgctc gtccagcagc acgagggcga
      721 tgtagggcac tgcgatggcc cgcttctgct ccaggttcgc gaaggccgca ttgcgagcgt
      781 tgttgcgggc cagcacgaag cccaccgcca gcaccagcac catcatcagc acggagacgg
      841 cggcgaacga ccacaatagc tttgaacgta gcgttctgaa catgggcaac gtaccgggcg
      901 tattacgcgg gcgtttcggc agcctgcaac accattttgt agccgacgcc gcgtatagtc
      961 tgcaaggtga gcgaggaacc ctggaggcgg tcgcgcaggt gggccacgtg cacgtccacg
     1021 gtgcgggtgt cgccgaaata gtcgtaaccc cagaccacgt tgagcagctt gtcccgctcc
     1081 agcaccaggc cgatatgttg cacgaaggcc agcagcaact cgaactcctt tgcccgtagc
     1141 tccacccgct tgccgccgac cgtcacctcg tgccgggcct cgtccaccgt ggtgttgccg
     1201 aactgtagtg gctgcccggt ctgcccggcg ctgtgccggg ccgccgtacg cctcaggacg
     1261 gccttgacgc gtgcgacaag ctcgcggggg ttgaagggct tggtcaggta gtcgtccgcg
     1321 ccaagctcca ggccgactat tttgtcaacg tcgtcgctgc gggcggtgag catgatgatg
     1381 ggagtgtcgc cctcgcgccg gatttgcctg cacacctccc aaccgtctac ctccggcacc
     1441 atcaggtcga gcaccacgag ggcaggggcg caggcacggt agcgttcgag cgcctgcctc
     1501 ccgttgttgg cctgctctac cctgaacccc tcgttcccca ggtacagctt taccagctcg
     1561 attatattcg gctcgttgtc cacaaccagg atagtcttgt cgctcacgca agcattatag
     1621 cacccggatt taggaattag gattgctgac ttaggatttg ttgtagggca acaccgaagt
     1681 cgaattcaga ggggaaggtg gcctttgtca ttctgagcgt agcgaagaat ctcgtccccc
     1741 agcactcaaa taccacttct cttactgtca ctatctatga caatctcccc aagaaagggg
     1801 taacatctcg gtggaggccg agattcttcg cgacgttcag a
//
LOCUS       DASRWZ010000192         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_769562, whole genome shotgun sequence.
ACCESSION   DASRWZ010000192 DASRWZ010000000
VERSION     DASRWZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769562"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..2153
                     /locus_tag="VFH60_02930"
     CDS             183..2153
                     /locus_tag="VFH60_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6312764.1"
                     /translation="MSVGQAQDSDSSIRSATEHFLTRARGRLVGRDGVLSIDGLDAAI
                     RAFGVATVMVLSLTGYGRVGGHFTDGLADPVGVALGLVLYNILVIAILGVPFRRPPTF
                     SLFLLDWSMVTTSVFLTGGFLSPFIILYYALGIGAALRVGFSRSMILVAACSVVYIFL
                     GVVSQADLGASKLPIVVIEIASLFMVVLTGVGMRNALEVEARRVQLEEQSVSQLRLLN
                     GLINTVLSASPDLEEVMRTVASVSSQALQSDSGLAVLFSEQVASPPEAAGGREIILVS
                     NSNPNPPQLSSIEDELLAQAVATQAPVLSDALRGDHTVRRYPGLERDGERLCSLVCVP
                     FMLEGRAIGGLFVGRFDQRAFTPTETGLLMAISSQVAVAVRLARLYEMEREKALRSQE
                     RERLERDLLSIVSHELRTPLTSIKTCIGALSDMSPTAPGAGATESTQTRLLQNVGRST
                     ERLIGLVNELLDMARLRAGRVSLQVQQVNIGTVITELAGQLLPLLESRGQTLLLDLPP
                     GDTGRWYRLSIPGDRRRLEQVLINLVSNANKYSPPGTRITLGATPREGQVRVFVRDEG
                     PGIPHAEQHRIFDKFYTGQEAIAPDERVESTGLGLAIARSIVELHGGTMSIASRPGSG
                     STFYFTLPQHATEGPAGLALVTNGAADEDTVG"
     gene            2134..2838
                     /locus_tag="VFH60_02935"
     CDS             2134..2838
                     /locus_tag="VFH60_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6312765.1"
                     /translation="MKILLVDDDPDVVEAITLSFNVHWPDATILGAGDGRTGVALFRQ
                     EDPDVVLLDIALPDTDGFRVLRQIREQSDVPVLMISARGEEVDKVKGLELGADDYVTK
                     PFGYMELTARIKAVLRRAQSLPPISGGGRFDMGPLAINYATHQVTLDGRPVKLTPIEY
                     KLLYQLTRSAGQVLLHDQLLTKIWGPEYLGELDYLRIYVRRLREKLEANPQQPEFILT
                     ERGLGYRFASPPVPAR"
     gene            2880..3992
                     /locus_tag="VFH60_02940"
     CDS             2880..3992
                     /locus_tag="VFH60_02940"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6312766.1"
                     /translation="MALDTTYAGVNPTGVGLYSRRLAEQVRIAAPGERLRLRCFGPAC
                     RPQADRPRRADVLQELPLNTQGVLPARLVRFRPNVVHATSHIGPVWGPGSMIVTVHDL
                     IFRRHPEDYPRAWLAATNLLLPRVLHRAAAIIADSQATAEDLHHFYRSTRGKVRVLYP
                     GFDEVAQPGLRQNPHDSTLRDLGIRGDPYIMCVGPWSRRKNLPVVLEAFDTLAREVPG
                     VLLVVSGNASSGMKGAQPQELIAGMQPETRARIKTVGFVPRDRLNVLLSGASLLAYPS
                     RIEGFGLPPLEAMSLGVPVVASDAPVLREVAGGSALHVPADDAAAWAAAFRRIFTEPG
                     LAASLSDKGRARSSQFSWRRCAAETIAVYREVAGRA"
BASE COUNT          706 a         1268 c         1311 g          770 t
ORIGIN      
        1 agacttcgtc tctggtaagc ccgccacttt agtggcaggg cggcccccgc agggctacca
       61 cgcagggcgg ccccaggcca cacgacgtta tctgcctgca cttttcgcgg aagtaccggc
      121 gtgcaccgcc ctggcgtata atggatgtga gtgaatactt cttacgtcac ggaaaataaa
      181 gaatgagcgt aggtcaagca caagatagcg atagctcaat ccgttcggcc acggagcatt
      241 ttctgacgcg agcgcggggg cggctcgtcg ggcgcgatgg cgtgctcagc atcgacggcc
      301 tggacgctgc catccgggct ttcggggtgg cgaccgtgat ggtgttgagc ctcactggct
      361 acgggcgcgt gggggggcac ttcacggacg gcctggccga cccggtgggg gtcgccctgg
      421 gcctcgtcct gtacaacatc ctggtaatcg cgatactggg tgtaccgttc cgccgcccgc
      481 ccaccttttc gctcttcctg ctcgactggt cgatggtcac gacctcggtc ttcctcaccg
      541 gcggcttcct aagtccattc atcatcctct attacgcgct tgggattggg gctgctttgc
      601 gcgtcggctt ttcgcgctcc atgatcctgg tggccgcctg ttcggtggtc tatattttcc
      661 tgggggtcgt gagccaggcc gacctggggg cctccaagct gcctatcgtg gtcattgaga
      721 ttgcctcgct gttcatggtc gtgctcaccg gggtcgggat gcgtaacgcc ctcgaggtcg
      781 aggcccgccg ggtgcagttg gaggagcagt ccgtcagcca gcttcgactg ctgaacggcc
      841 tcatcaacac cgtcctctcc gcatcgcccg acctggaaga ggtaatgcgc acggtggcct
      901 cggtctccag ccaggcgctg caatcggata gcggcctcgc ggtactcttc agcgagcagg
      961 tcgcttcccc acccgaggca gcagggggcc gcgaaataat actcgtgtcg aactccaacc
     1021 ccaatccacc tcagctttcg agcatcgaag acgaactact cgcccaggca gtggccacgc
     1081 aggccccagt gctaagtgac gcgctccggg gcgaccacac ggtccgccgc taccctggtc
     1141 tcgagcgtga cggcgagaga ttatgcagtc tggtgtgcgt gccgttcatg ctggagggac
     1201 gcgctatcgg cggcctcttc gttggacggt ttgaccagcg cgcgttcacc cccaccgaga
     1261 ccgggctgct gatggcgatt tcgagccagg tagcggtagc cgtgagactc gcccgcctgt
     1321 acgagatgga gcgcgaaaag gcgttgcggt cgcaggagcg cgagcggctg gagcgggacc
     1381 tgctttcaat cgtgtcccac gagctgcgta cgccgctcac gtccatcaag acctgtatag
     1441 gtgctctgag cgatatgtcg cccacagccc ccggtgctgg tgccaccgaa agcacgcaga
     1501 cccggctcct acagaacgtg ggacgcagca cggagcggct catcgggctg gtgaacgagt
     1561 tgctcgacat ggcgcgattg cgggcggggc gagtgtcgct ccaggtgcag caggtcaata
     1621 tcggcacggt aataacggaa ttggcaggcc agttgctccc gctgctcgaa tcgcgcgggc
     1681 agaccctgct gcttgacctg ccgcccggcg atacgggtcg atggtaccgc ctcagcatac
     1741 ctggcgaccg ccgccgcctc gagcaggtgc tcatcaacct ggtgtcgaac gccaacaagt
     1801 acagcccgcc gggcaccagg ataacgttgg gtgccacccc tcgcgagggg caggtgcgcg
     1861 tcttcgtgcg cgatgaaggt cccggcattc cccatgccga gcagcaccgg attttcgaca
     1921 agttttacac cgggcaggag gccatcgcgc ccgatgagcg cgtcgagagc accggcctgg
     1981 gactggccat agcccgctcc atcgtggagc tacacggggg cactatgtca atcgccagcc
     2041 ggcccggcag cggcagcacg ttctatttca ccctgcccca gcatgccact gaaggacctg
     2101 ccggtctcgc ccttgtcacg aatggagcag cagatgaaga tactgttggt tgacgatgat
     2161 cccgatgtgg ttgaagccat tacgctttcg ttcaacgtac actggccgga cgccaccatt
     2221 ttgggagcgg gcgacgggcg aacgggggtc gcgttgttca ggcaggagga ccccgacgtc
     2281 gtgctactgg acatcgccct gcccgacacg gacggctttc gcgtgctgcg ccagataagg
     2341 gagcagtccg acgtgccggt gctgatgatc tcggcgcggg gcgaggaagt tgacaaggta
     2401 aagggcctcg agcttggggc cgacgactac gtcaccaagc cgttcggcta tatggagctg
     2461 accgctcgca tcaaggccgt gctgcgccga gcgcagtctc ttcctcctat cagcggcgga
     2521 ggcaggttcg acatgggacc gctggcgatc aactatgcca ctcaccaggt cacactcgac
     2581 ggcaggccgg tcaagctcac gcctatcgag tacaagctgc tgtaccagct tacccgcagc
     2641 gccgggcagg tgctcctgca cgaccagcta ctgaccaaaa tttgggggcc ggagtacctg
     2701 ggcgagctgg actacctgcg catctacgtg cggcggttgc gagagaagct ggaagccaac
     2761 cctcagcagc cggagttcat cctcaccgaa cgcgggctgg gctaccgttt cgcctcgcct
     2821 cccgtgcctg caaggtagca ggggtgctgc ctggtcgaac gggtggcgca ccgcttaggg
     2881 tggcccttga caccacctac gcgggggtca acccgaccgg cgtgggcctc tattcgcgcc
     2941 gcctggcaga gcaagtgaga attgccgcgc ctggggaacg actgcgactc aggtgcttcg
     3001 gtcctgcctg ccgaccgcag gccgatagac cgcgacgggc agatgtgcta caggagttgc
     3061 cgcttaacac gcagggcgta ctacccgcgc gactcgtgcg cttcaggccc aatgtagtgc
     3121 atgcgacctc gcatatcggc ccggtttggg gtccgggcag catgattgtt acggtgcacg
     3181 acctgatctt ccggcggcac cctgaggact atccgcgggc ctggctggcg gcgaccaacc
     3241 tgttgctacc tagagtgctg catcgtgcgg cggccatcat tgccgactcc caggccacgg
     3301 cagaggacct tcatcacttc taccgttcga cgcgtggcaa ggtacgggtt ctatatcccg
     3361 gctttgacga ggtcgcgcag ccgggtctcc ggcagaaccc gcatgactct accttgcgcg
     3421 acctcggaat ccggggggac ccctacatta tgtgcgtcgg accctggtcg cgccggaaga
     3481 acctgcctgt ggtgctagaa gccttcgata cgctggcacg ggaggtaccg ggagtgctgt
     3541 tggtagtgtc tggaaacgcc tccagtggca tgaaaggtgc tcagccccag gagcttattg
     3601 ccgggatgca gccggaaacg cgggcgcgga tcaagactgt cggattcgta ccccgcgaca
     3661 ggttaaatgt gttactgtcg ggtgcaagtc tgttagcgta cccgtctcgc atcgagggat
     3721 tcgggctacc cccccttgaa gccatgtctc ttggggtgcc ggtggtcgcc tcggacgccc
     3781 cggtcctgag agaggtagcg ggagggtctg cgttgcacgt ccccgcggac gacgcagcag
     3841 cctgggccgc cgccttccgg cgcatcttca cggaaccggg ccttgctgcg tcactgagcg
     3901 acaaaggccg ggcgcgtagc agccaattta gctggcggcg gtgcgcggcg gagactatcg
     3961 cggtctaccg ggaagtcgcg gggagggcat aatcggctcg aatcgagagc cagcggcctt
     4021 ttacgccgtt tttatatgaa acattaagct ttttt
//
LOCUS       DASRWZ010000193         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_700924, whole genome shotgun sequence.
ACCESSION   DASRWZ010000193 DASRWZ010000000
VERSION     DASRWZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700924"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(543..1451)
                     /locus_tag="VFH60_02945"
     CDS             complement(543..1451)
                     /locus_tag="VFH60_02945"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HET6312767.1"
                     /translation="MKIKAIDILAAQRAALLAPAEESEAIYQEQVMEPWRSMWVPMLA
                     RMSQGGGNGEAASLMNAARMFGFYLPEHGVEQGLAALDVLERAGTWEACRRALEIAAD
                     ALKPGEHGIPLEQVQFSVVLMNPEMALKNEKAQGYSGFGSMPGYVMVTVWPNDYNVPR
                     LPSAAAHEFNHNVRLSYEPWSQETSVGQYVVLEGLAESFAGELFGSDKVGPWTQLPTE
                     ADMELARNRIREGLEVTGFNEIRGYIFGDWAAAAMGYTPQGLPDFAGYSIGYHIVQAY
                     MQRTDSTAAEATYLPWREIVEESRYL"
     gene            1572..>2347
                     /locus_tag="VFH60_02950"
     CDS             1572..>2347
                     /locus_tag="VFH60_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6312768.1"
                     /translation="MQSAIIDKLHTIGEVARLSGVPVKTIRYYSDIGLLPPSEVTEAG
                     YRLYSDADIVRLELVRTLRAVNFDLPTISRLLQGELRPADALELQLGAIEVQMRSLKR
                     QRSVLKATLLNVEKGEEAMLSHMKGTQSLAQLERLEREAFLKSRLDRGLEGVAVDPAW
                     KAAYLYSAISDLPEEMNEAQFEAWLELAALMSGEDFLSGVREQSRPFWEAVEGDFDMA
                     AWQAAQGQVFQAAMQAVSDGRSPGGEDAQQVVEQWVSLHAR"
BASE COUNT          408 a          718 c          745 g          476 t
ORIGIN      
        1 agcatgacta taagtccaag acagtctccc atggtcgatt acctcctcgg taacgcgtga
       61 gcgtaaagct cagagtaacg tgaacaacga tacgattgcc gccaggggca cggccaagac
      121 acctagcata gtgaaactgc aacagctaca gccgcctcgt ctacccgtgc gccgtagctc
      181 catcctcgta aggtcccgct cgtccatagc tatctccctc ttatgttgtc cccggtaggg
      241 ccggggccgg ttgtcggagt tcgccttccg tctttgcgcc ctttacaacg aaaacgaccg
      301 gctgcgtgtt atgcacgccg caaatcgtgt ttcggcgcag gccgtatcga ttatctaaac
      361 gccaagacgc caagattaag gatgttgtgt tctaagtaaa aggaccaggg tactacagca
      421 cgtaccctag tcctctttgt actctccaaa ccgtcatcct cttggcgcgt tctttgcgtc
      481 ctttgcgact ttgcgttttc gtactcttgg cgtggtcctt ggcgtcttga cgtttagatc
      541 tgctacaggt agcgggactc ttcgactatc tcgcgccagg ggaggtacgt ggcctcggcg
      601 gcggtgctgt cggtgcgctg catgtaggcc tgtacgatgt ggtagccgat gctatagccc
      661 gcgaaatcgg gtaggccctg gggagtgtag cccatcgcgg ccgcggccca gtctccgaag
      721 atgtagccgc ggatttcgtt gaagcccgtg acctccagcc cctcgcgtat gcgatttcgc
      781 gccagctcca tatctgcctc ggtgggcagt tgggtccacg gccccacctt gtcgctgccg
      841 aatagctcgc ccgcgaacga ctcggcaagc ccctccagca ccacgtactg gccgacgctc
      901 gtctcctggc tccacggctc gtagctgagc cggacgttgt ggttgaattc gtgggcggcc
      961 gccgagggca ggcgcggcac gttgtagtcg ttgggccaca ccgtcaccat cacgtagccg
     1021 ggcatggagc cgaagcccga gtagccctgg gccttctcgt tcttcagcgc catttccggg
     1081 ttcatgagca ccacgctgaa ctgcacttgc tcgagcggga tgccgtgctc ccccggcttt
     1141 agcgcgtcgg cggcgatttc cagcgcccgc cggcatgcct cccacgtgcc ggcgcgctct
     1201 agcacgtcca gggcggcgag gccctgctcc acaccgtgct ccggcaggta aaagccgaac
     1261 atgcgggccg cgttcatcag agaggcggct tcgccattgc cgccgccctg ggacatgcgc
     1321 gcaagcatcg gcacccacat cgagcgccac ggctccatca cctgctcctg gtatattgct
     1381 tcgctctcct cggccggtgc cagcagtgcc gctctctggg cggcgagtat gtctatagct
     1441 ttgattttca tagcggtgtc tccttcaata tccgggtatg attgccgcgc tgccatttac
     1501 ttgttgccgg tttgcagccg gacgagaaac agcataaact atccagtaac cggagagtca
     1561 aggggtaaaa cgtgcaaagt gccataatcg acaagctaca caccataggc gaggtggccc
     1621 ggctgagcgg ggtgccggtg aagacgatcc gctactactc ggatatcggc ctgctgcctc
     1681 cgtcggaggt gacggaggcc gggtaccgcc tctattcgga cgccgatatc gtgcggctgg
     1741 aactggtccg cacgctcagg gcggtcaact tcgacctgcc gaccatctcc cgcctgctgc
     1801 aaggcgagct acgcccggct gatgcgctgg agttgcagct tggggccatc gaggtgcaga
     1861 tgcgctcgct caagcggcag cggtcggtgc tgaaggcgac tctgctgaac gtggagaagg
     1921 gtgaggaggc gatgctctcg cacatgaagg ggacgcagag cctggcccag ttggagcggt
     1981 tggagcgtga agctttcctc aagtcgcgcc tggacagggg gcttgagggc gtggcggtgg
     2041 acccggcctg gaaggcggcg tacctgtact cggctatctc cgaccttccg gaggagatga
     2101 acgaggccca gttcgaggcc tggctggagc ttgccgcgct catgtctggc gaggactttt
     2161 tgagcggggt gcgggagcag tcgaggccgt tctgggaggc ggtggaggga gacttcgaca
     2221 tggcagcgtg gcaggccgcg cagggccagg tcttccaggc cgccatgcaa gccgtgagcg
     2281 acggccgctc ccccggaggc gaggacgcgc aacaggtggt ggagcagtgg gtgagcttgc
     2341 acgcccg
//
LOCUS       DASRWZ010000194         3781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_838641, whole genome shotgun sequence.
ACCESSION   DASRWZ010000194 DASRWZ010000000
VERSION     DASRWZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838641"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2165)
                     /locus_tag="VFH60_02955"
     CDS             complement(<1..2165)
                     /locus_tag="VFH60_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6312769.1"
                     /translation="MYKESVNAPQSLTTEPAHVTALMADLQILDERRHLAIERLRATV
                     GDAMVSAQRGLIIEEGDVDGWLTKRGDKESTVDQSGTARLPANRGGRLAALGSFFGLG
                     QLEQAMLLTTLAPEVDLSYANLYAYVQDDVTKKFPTLDLAVSLWCETLPDKLMARRLI
                     ASDGNLRRQLLLSVDDSPVLTSPLLARPMLLDPRIAAYLLGADDTDPALGNSVRVVRP
                     PAVESTLSSTQLRSLERLVRPIMGRAGPGVSRNGASSGNGSHAPVSSLVVWMRGLEKQ
                     GKRMAAEHLAAMLDCPMLVVDSAALVSAGQDQRKLMLRVLREARLQGAIIFWAEADAL
                     APTREGGRQDLPVDLANLLIEWPGCSFFDIRGVQPLHVPSVLSNELDFPIPTNERRRR
                     LWDYALAGVEVASDVDLALLTGAFKLSGEQIDAAADSARHAAAWRAASSGDKAEAHVT
                     MRDLLVACRQHSNQGLGLLARKVTPVYTWNDLVLPADRFAQLHEMCLHIRHGPLVFEE
                     WGFDRKLARGKGLNVLFAGQPGTGKTMAAEVLATDLGLELYKIDLSGVVSKYIGETEK
                     NLEKIFHEGQTSNAILFFDEADSLFGKRSEVKDSHDRYANLEISYLLQRMEEYDGIVI
                     LATNLRKNLDDAFIRRMHGAIEFPMPEEPDRLEIWQRTFPHEAPRDPNIDFAFLAKRF
                     KLSGGNIKNIVLEAAFFAAEAGTPISMGHVVRALRREHQ"
     gene            complement(2176..3414)
                     /locus_tag="VFH60_02960"
     CDS             complement(2176..3414)
                     /locus_tag="VFH60_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312770.1"
                     /translation="MMQPPSAIAEPIVVNLTPPEVKVAAGADPVEIIANVRNAGTTVD
                     QFSIELENIDSSWYTVLVQSVSLFPGDSAPIPIRIHPPKGSNTRAGHYTFVVRARSQA
                     DPTLVGVTKGVVQVGSYSVFQMELAPKRVTGRRGKYRLALTNGGNGETQLELTGRDAE
                     LTLNFGFNPSAPTIQPAQKKMVPVTVSKKGFRLVGMPEQYKFIINARPVDGNEKDAKE
                     VEGELVHQAWFTSWRRPLYILAALILLFLWLLFKPDLNPCSARFLLPSQIQFYSGFAC
                     VGGVLKPFAKNTAPPREVGQSENPCDSGPGFTEVRAKYKDLVGDCVSDEWSDDAGNNH
                     QTTTTGELLFAWIVRPDDSYAQAHPEVPRVGRESGELQARMYFIRNDGRLFTFVDCDP
                     RGTFNDCERVEIAPPQSAQE"
     gene            complement(3600..>3781)
                     /locus_tag="VFH60_02965"
     CDS             complement(3600..>3781)
                     /locus_tag="VFH60_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312771.1"
                     /translation="VRPAAGGGDRGYSGGGILDCVFDFQAISCYNGGSTGTGEKARAR
                     EEYIRHYTSAYSAGE"
BASE COUNT          669 a         1215 c         1135 g          762 t
ORIGIN      
        1 ttctggtgct cgcgccgcag ggcacggact acgtgcccca tacttatagg tgtgcccgct
       61 tccgccgcga agaaggctgc ctcaagcacg atgttcttga tgttcccccc gctcagcttg
      121 aaacgcttcg ccaggaaggc aaaatcaatg ttggggtcgc ggggcgcttc atgggggaag
      181 gtgcgctgcc atatctccag gcggtccggc tcctccggca tgggaaactc tatcgccccg
      241 tgcatgcgcc tgatgaacgc gtcgtccagg ttctttcgca ggttggtcgc caggataact
      301 atcccgtcat actcctccat gcgctgcaac aggtagctga tctccaggtt ggcgtagcgg
      361 tcgtggctgt cttttacttc gctcctcttg ccgaagagcg aatcggcctc atcgaagaag
      421 aggatggcgt tgctggtctg cccctcgtgg aatatctttt cgaggttctt ctccgtctca
      481 cctatgtact tgctaaccac gcccgacagg tctatcttgt aaagctcaag gcccaggtcg
      541 gtagccagca cctcggcagc catcgtcttg ccggtgccgg gctgcccggc gaagagcacg
      601 ttgaggccct tgcccctggc aagcttgcgg tcgaagcccc actcctcgaa caccaggggt
      661 ccgtgccgga tgtgcaggca catctcgtgc aattgcgcaa agcggtccgc aggcagcacc
      721 aggtcgttcc aggtataaac gggcgtcacc ttgcgtgcga ggaggcccaa cccctggttc
      781 gagtgctggc gacaggccac aagcaggtcg cgcatcgtca cgtgcgcctc cgccttgtcc
      841 cccgaagagg ccgcccgcca ggccgccgcg tgccgcgcgc tgtccgccgc cgcatctatc
      901 tgctcgcccg acaacttgaa cgcgcccgtg agcagcgcca ggtccacgtc ggacgccact
      961 tctacccctg ccagagcata gtcccacagc cgccgccgcc gttcgttggt gggtatcggg
     1021 aagtcaagct cgttggagag cacggagggg acgtggagcg gctgcacgcc ccgtatgtca
     1081 aagaaagagc agccgggcca ctcgatgagc aggttcgcca ggtcaacggg caggtcttgc
     1141 ctgccgccct cccgcgtcgg cgcgagcgcg tccgcttccg cccagaagat gatagccccc
     1201 tgtaaccgcg cttcacgcag cacgcgcagc atcagcttgc gctggtcctg ccccgccgaa
     1261 acgagggcag ccgagtccac cacaagcata gggcaatcga gcatcgccgc gaggtgctcg
     1321 gcggccatgc gcttgccctg cttctcaagg ccgcgcatcc acactaccag gctgctcacc
     1381 ggcgcgtgcg agccgttgcc cgacgatgcc ccgtttcgcg acacgcctgg tcctgccctc
     1441 cccatgatgg gccggaccaa ccgctcaagg ctgcgtagct gggtgctgct gagggtagat
     1501 tcgaccgccg gcggcctgac cacccgcacc gagttcccaa gcgcggggtc ggtgtcgtcc
     1561 gcgcccagca ggtaagccgc gatgcgcggg tcgagcagca tgggccgcgc cagcaggggt
     1621 gaggtaagca cgggagagtc gtctaccgag aggagcagtt ggcggcgcag gttgccatct
     1681 gaagcgatca gccgccgcgc catcagcttg tcgggcaagg tctcgcacca gagcgacacc
     1741 gccaggtcga gcgtggggaa cttcttggta acgtcgtcct gcacgtatgc gtagagattg
     1801 gcatacgaca ggtctacctc cggggccagg gtggtcagga gcatcgcctg ctccaattgc
     1861 cccagcccga agaagctgcc cagcgccgcc agccgccctc ccctattcgc ggggagcctc
     1921 gccgtgcccg attggtctac ggtgctttct ttgtcgcctc gcttggtgag ccagccgtca
     1981 acatcccctt cctcgatgat caggccgcgt tgggccgaga ccatcgcgtc gcccacggtg
     2041 gcgcgcagcc gctctatcgc caggtgtctc cgctcatcga gtatttgcag gtctgccatg
     2101 agcgcggtaa cgtgcgcagg ctcggtcgtt agagattgtg gcgcatttac agattcttta
     2161 tacatgactc ctgccctatt cttgcgctga ctgcggtggc gctatttcca cccgctcgca
     2221 gtcgttgaac gtgcctcgcg gatcgcagtc cacgaacgtg aatagcctgc cgtcgttgcg
     2281 tatgaagtac atacgcgctt gtagctcacc actctcgcgc cccaccctgg gcacttcagg
     2341 gtgggcttgg gcgtatgagt cgtcaggtct cacaatccag gcaaaaagca gctcccccgt
     2401 agtcgtggtt tggtggttgt tgcccgcatc gtcgctccat tcgtccgaga cgcagtcgcc
     2461 cacgaggtcc ttgtatttgg cgcgcacttc ggtgaagcca ggccccgaat cgcacgggtt
     2521 ttccgactgc ccgacttcgc gagggggagc ggtattctta gcgaacggct tgagcacgcc
     2581 gcccacgcag gcgaaaccgg agtagaattg gatttgcgaa gggagcaaga accgcgctga
     2641 acaggggttc aggtcgggct tgaagagcag ccacaggaag aggagtatga gggcggcgag
     2701 tatgtagagc ggcctgcgcc acgaggtaaa ccacgcctgg tggaccagtt cgccctccac
     2761 ctccttagcg tctttttcgt tgccgtccac cgggcgggcg ttgattatga acttgtattg
     2821 ctccggcatg cccactagcc tgaagccctt cttgctcacg gtgacgggca ccatcttctt
     2881 ctgcgcgggc tgtatggtgg gcgcggaggg gttgaagccg aaattgagcg tcaattcggc
     2941 gtcgcggccc gtaagctcaa gctgagtctc gccgttgccg ccgttggtga gagcgagccg
     3001 gtacttcccc ctgcgccccg tgacgcgctt aggggccaac tccatctgga agaccgagta
     3061 cgagccgacc tgcaccacgc ccttcgtcac ccctacgagg gtggggtcag cctgggagcg
     3121 ggcgcgcacc acgaatgtat agtgcccggc cctggtgttg ctccctttgg gcgggtggat
     3181 gcgtatcggg atgggcgcgc tatcgcctgg gaagagcgac acgctctgca caagcaccgt
     3241 gtaccaggac gagtctatat tctcaagctc tatgctgaac tggtccaccg tagtaccggc
     3301 gttgcgcacg ttggctataa tctccaccgg gtcagccccg gcggctactt ttacctccgg
     3361 cggtgtcagg ttgacaacga ttggctctgc gattgcagat ggcggctgca tcatagctct
     3421 ttatctcccc tctgactata tgatattggt tgacaacctt gcatgagcag agaggcggac
     3481 gctctatacg agcatcccct tatcctccct ggccaggcgt taatcctgaa ttacgaatta
     3541 caagtcagcc cggtcctgat tcacgatttg taattcgtaa ttagtaattc cattaccgcc
     3601 tactcgcccg cactatacgc actggtatag tgccggatgt attcctcacg ggcgcgggcc
     3661 ttttcgcccg tgcctgttga gcctccatta taacatgaga tggcctggaa atcgaaaacg
     3721 caatcgagaa tcccgccccc gctatagcca cgatcaccac cgccagcagc aggtcgaacc
     3781 c
//
LOCUS       DASRWZ010000195         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_15918,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000195 DASRWZ010000000
VERSION     DASRWZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15918"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..602
                     /locus_tag="VFH60_02970"
     CDS             192..602
                     /locus_tag="VFH60_02970"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HET6312772.1"
                     /translation="MSAPQDIKFRFNPSSERSSKVLGPLEDDIMEVVWSQGSATVSTV
                     HKALREKKDIAYTTVMTTMSRLAKKHLLNQDTTNSSYVYTPTLSRDDFERYVVRGVVN
                     GLFDDFGDEVIDYFLECVRQRGDGALAKLRSAVG"
     gene            805..1668
                     /locus_tag="VFH60_02975"
     CDS             805..1668
                     /locus_tag="VFH60_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6312773.1"
                     /translation="MFSWFGKKRKATAERLPDAPEPTVREALLSARPSDIFARWAHKG
                     SLATILPDLDALRKVSQLPAHRDNAFIHTLKVVDAIEPTPVRRWAALLHDIGKGPTFI
                     ETPEGRSRFFNHDLVGTVMAEEIMSEHGEDPALIEAVQRLVSLHMRPIAYRADEWTDA
                     AVRRLVEEAEEGRGREGWDDLLALSHADLRGYLPEPIDRGLWVLQSLEERRRQIDEAE
                     AREAQLSSLGPRSPLDGTELLALSAREPGPWVGQLKDYLCNEVEARRLGQEDKVAATE
                     LAQRWLETHTG"
     gene            2029..2754
                     /locus_tag="VFH60_02980"
     CDS             2029..2754
                     /locus_tag="VFH60_02980"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6312774.1"
                     /translation="MRIFRTPPYQDVTELLDSISGVDSHDLQTTLRDIRRANIFGLGT
                     WVVKHHLEKLLGARGHTGKLSVLDVATGSGDIPEELFRWAGRRGIELSAVLTDISPEI
                     LQVARERINQAGFGGSSSFVVCDAGKLPFADASFDVVVCSLAFHHLTLQQGKAALREM
                     ARLARVGFIINDVYRSQGAWYMAWALVHLTTTNRLTRHDGPASVLRAFTPRELRRMAA
                     EVGVPVSVYTHPFWRVAVVGPSG"
BASE COUNT          538 a          842 c          868 g          520 t
ORIGIN      
        1 gcttttgcag tccctactca gtcctcagtc ctcattactg gtccttgcta ctaagggtag
       61 cgggacatta cgactcgtgg taaaatgggc atgccggatg cgttttcctg gcctcttact
      121 tcctgcaaag tggcgcacgg caatggaagc tttggcttcc gcgtaccata tttgcgtatc
      181 aggagcagga aatgagcgcc cctcaggaca tcaagtttcg attcaaccct tcatctgagc
      241 gttcgagcaa ggtgcttgga cccctcgaag acgacatcat ggaggtggtc tggtcgcagg
      301 gcagcgccac ggtgagcacc gtacacaagg cgctccgcga gaagaaggac atcgcctaca
      361 cgaccgtcat gaccaccatg agccgccttg ccaagaagca cctgctcaac caggacacca
      421 ccaactccag ctacgtctac acgcccaccc tctcgcgtga cgacttcgag cgttacgtgg
      481 tgcgcggcgt ggtcaacggc ctcttcgatg actttggcga cgaggtgata gactacttcc
      541 tcgaatgcgt gcggcagcga ggtgacggtg ccctggcgaa actgcggtca gccgtcggct
      601 agtccactgc ttctctcctg ctcaacgtca gttcccggta cgggcacgac ccgccacttc
      661 acgccggtcg ccccaccctg agaattctaa cccatctttt gggcatgtgc cttgcagtag
      721 tgcgtaccgg gtgaacgccc gcgtatcgta accgtacgcg tgcacggcca cgcctgcatc
      781 ctgagagaag accccttgcc cgatatgttt tcctggtttg gaaagaagcg caaagccaca
      841 gcggagaggc tgcccgacgc gccggagcct acggtgcgag aggcgctgct ttcggcgcgg
      901 ccctccgaca tattcgcgcg ctgggcccac aagggtagcc tagctactat cctgcccgac
      961 ctcgatgcgc tgcgtaaggt gagccagctc ccggcgcacc gcgataacgc cttcatccac
     1021 accctcaagg tggtggacgc gatagagcct acgcccgtga ggcgctgggc cgccttgctg
     1081 cacgacatcg gcaaaggtcc gactttcata gagaccccgg agggccgctc ccgcttcttc
     1141 aatcacgacc tcgtgggcac cgtcatggcc gaggagatca tgtcggagca tggagaggac
     1201 cccgcgctga tcgaggcggt gcaaaggctg gtgagcctgc acatgcggcc gattgcatac
     1261 cgcgccgacg aatggacgga cgccgccgtg cgccgcctgg tggaggaggc ggaggaaggg
     1321 cgtgggaggg agggttggga cgacctgctg gccctgtcgc acgccgactt acggggctac
     1381 ttgccggagc cgattgaccg cgggctgtgg gtgctgcaaa gcctcgagga gcggcgcagg
     1441 cagatagacg aggccgaggc gcgggaggcg caactctcca gcctcgggcc gcgctctccc
     1501 ctcgatggca cagagttgct ggcgctctcc gcccgcgagc ctggaccgtg ggtgggccaa
     1561 ctgaaagact acctctgcaa tgaagtagag gcccggcggc tcggccagga agacaaggtg
     1621 gcggccaccg agcttgccca acgctggctt gagacgcaca ccggctgagc ggtgttagaa
     1681 gccatttcaa gaatgacaga ctagcatttg agcacccaag ctcccctcct gtcatttcga
     1741 acgtagtgag aaatctcgtc cctcagcaca ttgttccacc tttcttcgag tacagagtct
     1801 catgagaaaa ggggaacctc ccggtagagg acgagatttc tcgctgcgct cgaaatgaca
     1861 taggcggggt ttacgtctca aggttcggca gtgttgtttt tgagatggct tatagagagc
     1921 cagaagtcaa ggcgggcgag ctgcattgtc cgccgaagaa gggtggccgt gcattggtct
     1981 tgccttcgga ttgacagcct accccatgca acatagaatg acactagcat gcgaatcttc
     2041 cgcacaccgc cctaccagga cgtcaccgag cttctggact cgattagcgg cgtagacagc
     2101 cacgacttgc aaacgacgct ccgcgacatc cgcagggcca acatcttcgg cctgggcacg
     2161 tgggtggtca agcaccacct ggaaaagctg ctcggggcgc gcgggcacac cggcaagttg
     2221 tcggtgctcg acgtggcgac ggggtcgggc gacataccgg aggagctttt tcgctgggcg
     2281 gggaggcggg gtatagagct atcggccgtg ctaaccgaca tcagccccga aatcctccag
     2341 gtcgcccgcg agcgtattaa ccaggcgggg ttcggtggct cgtccagctt cgtggtatgc
     2401 gatgcgggta agctgccgtt cgcggacgcc tcgttcgacg ttgtcgtgtg ctcgctggct
     2461 ttccaccatc tgacgctcca gcagggcaag gccgccctcc gcgagatggc acggctggca
     2521 agggtgggtt tcataatcaa cgacgtatac cgctcgcagg gagcctggta catggcctgg
     2581 gcgctggtgc acctcaccac caccaaccgc ctcactcggc acgacggccc ggcctcggtg
     2641 ttgagggctt tcacaccaag ggagctaagg cgcatggccg cggaggtggg cgtaccggtg
     2701 tctgtgtaca cgcacccatt ttggcgagtg gcagtagtgg ggcctagcgg gtagccgatg
     2761 taggatgg
//
LOCUS       DASRWZ010000196         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1181153, whole genome shotgun sequence.
ACCESSION   DASRWZ010000196 DASRWZ010000000
VERSION     DASRWZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181153"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..2153
                     /locus_tag="VFH60_02985"
     CDS             132..2153
                     /locus_tag="VFH60_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HET6312775.1"
                     /translation="MQAIAETRNTGAESFEMSNTRVVIENVKPEIDGGRFPIKRVIGE
                     KVQVTADIFTDGHEAVSAVLLYRKSGGDWTEMPLTFLVNDAWEGEFTVSEIGQWEYTV
                     AAWLDHFKFWQRAMLKRIDAKQDVSVDLVIGANLVEAAVTNAQGDDRKRLSAYAEALR
                     RGGTEALQPAVSEELAQLMYRYESREPLTRYEPVLRVTVDPTRARFSQWYEFFPRSCW
                     EPGCEHGTFADCENRLQYVHQLGFNVVYLPPIHPIGHTKRKGKNNSVVAQPGEEGSPW
                     AIGSEEGGHKAIHPALGSLDDFRRLVRRARDEYGIEIAMDIAFQCSPDHPYVKEHPDW
                     FRMRPDNTIQYAENPPKKYEDIYPFDFESRDWRNMWEELRSVLLYWIEQGVRVFRVDN
                     PHTKSLMFWEWCIGTIKERHPETIFLSEAFTRPKLMYYLAKIGFSQSYNYFPWRNARC
                     ELTEWMTELTTTEVKEFYRPNLWPNTPDILTEYLQVSGRPGHMIRFLLAATLGASYGI
                     YGPAFELCVNQPIAYGKEEYLDSEKYEIKHWDVSSPLTLAPLITRVNKIRRENKALQT
                     NEGLRFHQTTNDKLIAYSKSTPAGDNIILTVVSLDPVYTQSGFVEVPLEEFGMDPHQP
                     FPVHDLLTDNRFMWAGRSNYVELNPSILPAHVLSLRTRARTEQDFDYYM"
     gene            2474..4120
                     /locus_tag="VFH60_02990"
     CDS             2474..4120
                     /locus_tag="VFH60_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HET6312776.1"
                     /translation="MSDNGNFGPMKSWEEVFQGKNKAALEKLLPAYLMTVRWFGGKAR
                     QIGGVTLTASIPLPCEQLKPYIANIRVDYEDGGNQTYVLPLDFAQGKRGEQIESDSPQ
                     AVIARFEVGGTEGMLYDASQDKAFAALLLDSVRKGEIFGDSAYSLVAWPTPAFAHVVG
                     EEDEHVEPKIMKAQQSNTSIAYGGRAILKMFRRLEEGTSPDLEIGRFLGEQGFAHTPP
                     LAGALEFKTGDGDEPLTLAILQGFVSNQGDAWEYTLDSLEGYFESAASGGADNVPESP
                     RGEHLLEMLDQEVPAPVREALGPYLDSARLLGRRTAEMHIALASGANDPAFAPEPFTR
                     EYLRSRYRSVSGLTEQAFGLLENKAADLPGETREQARQVIESRQQILSRFEAMLQGQI
                     DAVRTRVHGDYHLGQVLWTGSDFVIIDFEGEPVRSLKERREKHSPLKDVTGMLRSFHY
                     AAYAAMFSRITKDVVASESQAGIEGWADAWHMWVSVAYLKEYLEVAGSGTSLPRNPAD
                     LRTLFDIYLLEKAVYELIYELNNRPDWVRIPLQGVRELVG"
BASE COUNT          854 a         1356 c         1418 g          748 t
ORIGIN      
        1 gttaacctgc cggggccttt ttgcttgccg agtcattgcg gcatataaaa tgcagactat
       61 agcccatgtt cgcgcatgac gccgcgagcg taccaggagc aggactctag agtaagagag
      121 ggttagcagc aatgcaggct atagctgaga cgcgcaacac cggggctgaa agcttcgaga
      181 tgagcaacac gcgcgtggtc atcgagaacg tcaagccgga gatcgacggc gggcgcttcc
      241 cgatcaagcg cgtgattggc gagaaggtgc aggtaaccgc cgacatcttc accgacgggc
      301 acgaggcggt atcggccgtg ctgctctacc gcaagtcggg cggcgattgg accgagatgc
      361 cgctcacctt cctggtaaac gacgcctggg agggtgagtt cactgtctcc gagatagggc
      421 agtgggagta caccgtcgcc gcctggctgg accacttcaa gttctggcag cgggccatgc
      481 tcaagcgtat cgacgcaaag caggacgtga gcgttgacct ggtgataggc gcgaacctcg
      541 tagaggccgc cgtcaccaat gcccagggcg acgaccgcaa gcggctatcc gcctacgctg
      601 aggcattgcg caggggcggc acggaggcgt tgcagcccgc ggtgtcggag gagcttgccc
      661 aactgatgta ccggtacgag tcgcgggagc cgctgacccg gtacgagcct gtgctgcgcg
      721 tcaccgtgga ccccacccgc gcacgcttca gccagtggta cgagttcttc ccccgctcgt
      781 gctgggagcc gggctgcgag cacggcacgt tcgcggactg cgagaaccgc ttgcagtacg
      841 tacaccaact tggcttcaac gtggtctacc tgccgcccat ccacccgatt gggcacacca
      901 agcgcaaggg caagaacaac agcgtggtgg cgcagccggg cgaggagggc agcccctggg
      961 ctataggctc ggaggagggc gggcacaagg cgatacaccc cgcgctcggc agcttggacg
     1021 acttccgcag gctggtgcgc agggcgcggg atgagtacgg catagagata gcgatggaca
     1081 tcgcgttcca gtgctcgccc gaccacccat acgtgaaaga gcaccccgat tggttccgca
     1141 tgcgccccga caacacgatc cagtacgccg agaacccgcc caagaagtac gaggacatct
     1201 acccgttcga cttcgagagc cgcgactggc gcaacatgtg ggaggagctg cgcagcgtgc
     1261 tcctctactg gatcgagcag ggcgttaggg tgttccgggt ggacaacccg cacaccaagt
     1321 cgcttatgtt ctgggagtgg tgcatcggca cgatcaagga gcggcacccg gagacgatct
     1381 tcctgtccga ggcgttcacc cgccccaagc tgatgtacta cctggccaag ataggcttca
     1441 gccagtcgta caactacttc ccctggcgta acgcccgctg tgagcttacc gagtggatga
     1501 ccgagcttac tacgaccgag gtgaaggagt tctaccgccc caacctgtgg cccaacaccc
     1561 ccgacatcct gaccgagtac ttgcaggtca gcgggcggcc cggccacatg atccgattcc
     1621 tgctggcggc cacgctgggc gcgagctacg gcatctacgg ccccgcgttc gagctttgcg
     1681 tgaaccagcc tatcgcctac ggcaaggagg agtacctcga ctcggagaag tacgagatca
     1741 agcactggga cgtaagcagc cccctgacgc tcgcacccct tatcacccgc gtgaacaaga
     1801 taaggcgcga gaacaaggcg ctgcaaacca acgagggcct gcgcttccac cagaccacca
     1861 acgacaagct gatcgcctac agcaaatcca cgcccgcagg ggacaacatc atcctcaccg
     1921 tggtgagcct ggacccggtg tacacgcagt cgggcttcgt ggaggtgccg ctggaggagt
     1981 tcggcatgga cccgcaccag ccgttcccgg tgcacgacct gctcaccgac aaccgcttca
     2041 tgtgggcggg ccgcagcaac tacgtggagc taaaccccag catcctgccc gcccacgtgc
     2101 tcagcctgcg cacccgcgcc cgcaccgagc aggacttcga ctactacatg tgaggctatg
     2161 gagcagtggg gcacgatacc ccactgctcc tgacgggatg ccacgttgag cgggaccaga
     2221 gtcccctctg tcatcctgag cgcagcgaag gatctaaaga aggtggtaag gtgtgctgtt
     2281 gaatgtagtg gcaatggtgc tgacggttca acgcatgggc aagggcagca tcttagatcc
     2341 ttcgctgcgc tcaggatgac aggggggact ctggtcgccg ggaccatgtc ataggggtgg
     2401 cctgccagga tgtgctgcat cccgccgctc cgatgggagc ggatatccaa acccgtaatg
     2461 aggtattgca aacatgtctg acaacggcaa cttcggcccc atgaagtcgt gggaggaagt
     2521 gttccagggc aagaacaagg cggcgcttga gaagctgctg cccgcttact tgatgacggt
     2581 gcgctggttc ggcggcaagg cccggcagat agggggcgtt acgcttaccg ccagcatacc
     2641 cctgccctgc gaacagctta agccctacat cgccaacatc cgggtggact atgaagacgg
     2701 cggcaaccag acctacgtgc tgcccctcga tttcgcccag ggcaagcgcg gcgagcagat
     2761 agagagcgac tcgccgcagg ccgtaatcgc ccggttcgag gtgggaggta cggagggcat
     2821 gctctacgac gcctcgcagg acaaggcgtt cgccgccctg ctgctcgact ccgtcaggaa
     2881 gggtgagatc tttggcgata gtgcctattc gctggtggcc tggcccacgc ctgccttcgc
     2941 gcacgtcgtc ggtgaggaag acgagcatgt cgaaccgaag attatgaagg cccagcagag
     3001 caacacgtcc attgcttacg gggggcgtgc catcctcaag atgttccggc gcctggagga
     3061 gggcaccagc cccgacctgg agataggccg cttcctgggg gagcagggct tcgcgcatac
     3121 ccctcccctc gcgggcgcgc ttgagttcaa gaccggggat ggcgacgagc cgctgaccct
     3181 ggcgatcttg cagggcttcg tgtccaacca gggcgacgcg tgggagtaca ctctcgattc
     3241 gttggaaggc tacttcgaga gcgcggcgtc cggtggcgct gacaacgtgc ctgagagtcc
     3301 gcggggagag cacctgctgg agatgctgga ccaggaggtg cccgcgccgg tgcgggaggc
     3361 cctcggcccc tacctcgact cggcccggct gctgggcagg cgcaccgccg agatgcatat
     3421 cgccctggcc tctggtgcca acgacccggc gttcgcgcct gagcctttta cgcgggagta
     3481 cttgcgctcg cggtaccgtt cggtgagcgg gcttacggag caggctttcg ggttgctgga
     3541 gaacaaggcg gcggacctgc cgggcgagac gcgagagcag gcccgccaag tcatagaaag
     3601 ccgccagcaa atcctgtcgc ggttcgaggc gatgttgcag gggcagatag atgcggtgcg
     3661 cacccgcgtt cacggcgact accacctggg gcaggtgctc tggaccggca gcgacttcgt
     3721 aatcatcgac ttcgagggcg agccggtgcg ctcgttgaag gagcggcgcg agaagcattc
     3781 gcccctcaag gacgtgacgg gcatgctgcg ctcgttccat tacgccgcgt atgccgccat
     3841 gttctcccgc attactaagg acgtggtagc ctccgagtcg caggcgggca tagagggctg
     3901 ggccgacgcc tggcacatgt gggtcagcgt ggcttacttg aaagagtacc tcgaggtcgc
     3961 cgggtcgggc acctccctgc cgcgcaaccc cgccgatctc aggacgctgt tcgacatcta
     4021 cctgctcgaa aaggcggtgt acgagctaat ctacgagctg aacaaccgcc ccgactgggt
     4081 acgtattcca ctacagggcg tgcgcgagct tgtggggtag agcttattct tgagatttga
     4141 cgggcagcac ggtgctatat tcccgcttga atgaccacac gcttactcta gccaggccct
     4201 gagcgccgcg gcttatctca cttctatctc ggccagcaca acccggtccg gcagcacggt
     4261 cgcctggcct tccgttggcg ctgcctcagc ctgcgagcgc acggctgctc cggggccgtt
     4321 ggggggcacc cacatgcccg tttcgagacg gtacttgccg ggtttgaggt cggcgg
//
LOCUS       DASRWZ010000197        11496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_769970, whole genome shotgun sequence.
ACCESSION   DASRWZ010000197 DASRWZ010000000
VERSION     DASRWZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11496
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769970"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFH60_02995"
     CDS             <1..535
                     /locus_tag="VFH60_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET6312777.1"
                     /translation="EQERLRAVLLAATEHFGWASRGSGRLPGHGYGLACGTEKGSFVA
                     TCAEVEVERGTGRLRVVRVIEAFECGAIVNPQGLANQVEGAVVQALGGALFERVEFGG
                     GNVESNRFSRYRVPRFADVPQIEVVLVDRKDLPSAGAGETPIVGPAPALANAIFDACG
                     LRLRDLPLAPGGLLAGT"
     gene            complement(849..1769)
                     /locus_tag="VFH60_03000"
     CDS             complement(849..1769)
                     /locus_tag="VFH60_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6312778.1"
                     /translation="MGLQLRPHHPGEHGNHFDTTLAVVRACERAGLDSAWMADHFMFA
                     DAEHPEKEVPVLECFVVLGALAAATSSIRLGQLVAGVPYRNPALLAKMCATLDVISHG
                     RSIVGLGAAWHEGEFTAYGWPFPPARERLEMLEEAVQVVDRMLRQRPASFTGKHYSIE
                     NAYNDPRPVQQPRPPIMIGGSGEQVTLKLVARYADFCNVGGDPATVARKFDVLRRHCE
                     AVGRPFSEITLCNNVGIVLGRDEAEVAAKLEKYPGFGDIQGTPETVVARLREYAQVGS
                     RYVTFHMPDAEELDTIRLLGEEVLPQVAEM"
     gene            2124..3791
                     /locus_tag="VFH60_03005"
     CDS             2124..3791
                     /locus_tag="VFH60_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET6312779.1"
                     /translation="MVLCGLEGLGLRTLEELHRLGEGVVVVAASPSEQFAARAEALGA
                     TLIRGDYTQESVLKSAGVESADAIVLTDSSDLGNLHAALTAQELNPRIRVVLRMFNDE
                     LGKRIQALFYDCHAFSASAIAAPSFVSAALYQDWEQQIYLEGRMLVVHQASTADTNVV
                     LPLACVESDGTTTLFPEDQHEAICLVDSGLRPDEVGEGPHSKDRAPRGRLARRVRRLG
                     TAFGALGAMADRKFAYLLLALTLLACLGTIVFYSFARLDFVQAFYFTVTILIGTGFDQ
                     ITIPDAPLALEFFGVFVVLLGAVALTLFYTFITDALVGARLARLLGAAHGRMKGHVVV
                     CGVGSIGYRVVEQIARQGIPVVAAELHENDFIHTIRRLGVPVVMADVRLKETLEELDV
                     GEARCLVAATDDDLANLQAALNARAINPTLRVVLRVFDPDLAARVERAFSIRVSRSVS
                     SLAAPTFAAAAVGGKVIATAAVGKRVLIVGQGRIERGSWAEGKNLEQLQRAIGGRAIL
                     LSADGHKTWRPDRATVLSKGQELVVATTRAGFARFVESVEGHERSQE"
     gene            4052..5236
                     /locus_tag="VFH60_03010"
     CDS             4052..5236
                     /locus_tag="VFH60_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6312780.1"
                     /translation="MRHLLIDNVRVWDGTGATTQAGQAVEVQDGRIAWVGPASDWPGR
                     RAGVAIVDGRARTLMPGLIDCHVHYASPGGPDWIERFTDPEPEVTLRAIDLAGASLRS
                     GVTSARDVGAPDDLNIRLSKAAASGAFPSPHIHAAGTWIAHQGTYVSFARQFATADEL
                     RQAISVEVEAGADLIKVALAPWNEGKRPEGAPAVPFDADLLAVAVEAAHAAGLSIACH
                     ANDPESCKLAARAGVDSLEHGMHLDGDDLAAMATHGTVLVPTLSVWDSWLFYTREVGW
                     PEARKARAESLRESSRAAVSGAAKAGVPVALGTDAGGGSVRHGRVAREVELMIECGLS
                     PEAALQAGTTVAARLLGVSDRGAIEVGQVADMVLLDANPLDDPAALRLVAAVFQGGRR
                     VA"
     gene            complement(5290..5964)
                     /locus_tag="VFH60_03015"
     CDS             complement(5290..5964)
                     /locus_tag="VFH60_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HET6312781.1"
                     /translation="MKLSNFASIDPNHPGRALYRGGQGDRDVWEEFISNPEGLAREVA
                     RIRRQLGAIEQPIDEEDYHTPIPELVEVHQYIAQRAGRKSSGQGYSVDPGTRRAIELH
                     AMNFAIEYYIARGWHVEDVSATMSYDLRCTQPTLDDTDELHVEVKGTTSRGSGVLLTP
                     NEVIHARTYSNVALCIVSEIIVTRNADQLPIATGGTMRIIYPWDINVGQLTAVGYEYT
                     IPPNDI"
     gene            6475..6903
                     /locus_tag="VFH60_03020"
     CDS             6475..6903
                     /locus_tag="VFH60_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6312782.1"
                     /translation="MAAEVGPGAEYVIPMERLESFYDEPGEYGWIMEGHKHGFGLSSI
                     IITQTAPRGGPPLHVHDTEEVHVLPECHLAYVIGESTFEVEGPCVVNIPARVPHTFLN
                     IGPAVARIVCFWPANDFWTNYDELGPNPLLARYGAGGQAV"
     gene            6934..7500
                     /locus_tag="VFH60_03025"
     CDS             6934..7500
                     /locus_tag="VFH60_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312783.1"
                     /translation="MEIESLSCNNCGAPLDVPSTANFLTCRYCGSRLVVRRTETAHYT
                     ELMEQLERSTQQLEDQAEYMRLRDTVDALDADWQRERRRHLIRRPNGSLQSPTRRYLG
                     TSIVVALVVFGLTGLWFYASASAGPGASCFGWIGAVISLAAVAHAAWVSNKVSNYNLA
                     KRVYHQERGELLKRMYEVYPQELDWVRE"
     gene            7550..8908
                     /locus_tag="VFH60_03030"
     CDS             7550..8908
                     /locus_tag="VFH60_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6312784.1"
                     /translation="MTGTNDHIDSFLQERLDRYIDETARLCAQPSVSATGEGVAECSN
                     IVAEILAGHGFEVQKFDTPGEPVVVGRARGRSERTLLCYNHYDVQPAEPFELWTSPPF
                     EPTVRDGALYARGAKDDKGEFIARLAAVDAVRAAHGGELPCGVTFVVEGQEEIGSPHI
                     ARFVLDHLDLLKSHGAIWEEGGVGPEERPFNVLGARGVLTVEFHVRTLSRDAHSGQGS
                     VLPNAAWRLLWALTSLKGPDGRIRIPGFYDQALPASQLDKDLFAALPSGEHEARETFG
                     VKEFLHGVTGEAYKLAVFEPTCNVQGIGSGWQGVGPKTIVPAEASAKVDFRLVPDQDP
                     DDILEKLRRHLESEGFGDVEVTRLGAMWPAKSAPDDPLVLLTNRAAEEVYGVPSLVMP
                     ISGGSSPIYAFAGPLGIPVVTAGVGYLGSRTHSPDEHVRLDHFLNGARHIARIVDGFA
                     EI"
     gene            complement(9060..>11496)
                     /locus_tag="VFH60_03035"
     CDS             complement(9060..>11496)
                     /locus_tag="VFH60_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6312785.1"
                     /translation="LLGKRVFEAYPHVDQAGFKVNYREAWKIQAAVEYEAYYEPFETW
                     FAFNAQPHEGGGLSVYFRDITARKQAETERLRLAAIVESSEDAILAKTLDGVITSWNR
                     GAEKMYGYTAEEMVGRSIYVLVPVERHSEVNSILGLIRKGERVEAYETVRLTKTGDHI
                     TVSVSVSSILDEAGAIVGASAIARDMTARIRAEKALEAKERHFRSIVMSLQEGLVVQD
                     ASGCIVECNERAEQVLGLKRDQLIGRSSIDPCWRAVREDGSPFPGEDHPAMVSLRTGG
                     FLTNVPMGVRKPDDTLAWISINSHPLFNPGETVPYAVVVTFSDMTERRAAEEQIRIQT
                     QVLDQIQAGIVVMNMERRVTHWNAYAEKLYGWKQEEVLGKELRELLIMPPDQPKAKQV
                     MEQVFREGYWEGEFTLQHKNGTTFPAYVIDTVIRDGNGAPIAVAGISRDITERNEREQ
                     RLQRQFDHLAAMHSIDKVITASLDLPVTLDVILEQVTSQLGVDAADVLLLNPHTQSLE
                     CVAVRGFRHSDVQKTRMRLGEGFAGRAALERHTIHIPNMLNRKQPMLFSVAEREGFLM
                     YHGVPLIARGKVQGVLEIVHRSPLDPDAAWLQFSDALARQAAIAIDNATLFQELQRSN
                     TELALAYDNTLEGWSRALDLRDKETEGHTRRVTDVTVRLARSMGVGEAGVLQIQRGAL
                     LHDIGKMGIPDSILLKPGPLTDEEWEIMRRHPVYAYELLSPISFLRPALDIPYCHHEK
                     WDGTGYPRGLKGEQIPLAARIFAVVDVHDALSSDRPYRAAWPPEKVREHIRMGAGTHF
                     DPDVVDAFLTRNE"
BASE COUNT         2125 a         3512 c         3643 g         2216 t
ORIGIN      
        1 ggaacaggag cgcctgcgcg ccgtgctgct agcggcgacc gagcatttcg gctgggcctc
       61 cagggggtca gggcggctac cggggcacgg ctatgggctg gcctgcggca cggaaaaggg
      121 gagctttgta gccacgtgcg cggaggtgga ggtcgagcgg ggcacggggc ggttgagggt
      181 ggtgcgggta atcgaagcgt tcgagtgcgg ggcgatagtc aacccgcagg ggctggccaa
      241 ccaggtagaa ggggcggtag tgcaggccct gggtggcgcg ctcttcgagc gggtcgagtt
      301 cggcggcggc aacgtagaga gcaaccggtt ctcgcggtac cgggtgccga ggttcgcgga
      361 cgttcctcaa atagaggtgg tgctggtgga ccgcaaagac ctaccctcgg cgggtgcggg
      421 cgaaacaccc atcgtcggcc cggcccccgc gctcgcaaac gccatattcg atgcctgcgg
      481 cctgcggctg cgcgacctgc ccctcgcgcc aggtgggctg ctggctggaa cgtagcggaa
      541 atcgggggcc acgcggccgg tatcacgaca gccaccggat accgaggcag agccaggaag
      601 agttaaacaa aagacccaaa caatccgtga ggaccgtctg ggcttttgtt cctccgctgt
      661 cacaggcgaa cctgcaacct cgaagtaggc tcattatagc tacctagctt ggctgagtca
      721 acgaggagcg gcgggacttt tacacatcca tcctgtaatt ggaaggggag gtgcacggac
      781 caagccaatt acaggccagg gttgatccat atcgccacac ggcctaaaga accgctaacc
      841 ccggcgactc acatctccgc cacctggggc agtacctcct cacccagcag gcggattgtg
      901 tctagctcct cggcatcggg catgtggaag gtgacgtacc gggagcctac ctgcgcgtac
      961 tctctcaatc gcgcaaccac cgtttcgggc gtgccctgga tgtccccgaa gccggggtac
     1021 ttctccagct tggccgctac ctctgcttcg tcgcgcccca gcactatccc aacgttgttg
     1081 cagagggtaa tctcgctgaa aggacgtcct accgcctcgc agtgccggcg caacacgtca
     1141 aacttgcgtg cgacggtggc cgggtcgcca cctacgttgc agaagtcggc gtagcgggcg
     1201 accagcttga gcgtaacctg ctcgccgctg cccccgatca tgatgggcgg ccggggctgt
     1261 tggacgggcc tggggtcgtt gtaggcgttc tcaatcgagt aatgcttgcc ggtgaagctc
     1321 gcgggccgtt gcctcaacat ccggtccacc acctgcacgg cttcctccaa catctccagc
     1381 ctctcacgag cgggtgggaa gggccagccg taagcggtga actccccctc gtgccaggcc
     1441 gcgcccagcc ccacaatgct gcggccgtgg ctgattacgt caagcgtggc gcacatcttc
     1501 gctagcagcg ccgggttgcg gtagggcaca ccggccacaa gctgccccag ccttatgctg
     1561 ctcgtagccg cggccagcgc ccccagcacc acaaagcatt ccagcaccgg cacctctttc
     1621 tccgggtgct ccgcatcggc aaacatgaaa tggtccgcca tccaggccga gtcgagccct
     1681 gcacgctcac aggcccgcac gaccgcaagt gttgtgtcga agtggttgcc gtgctcgccg
     1741 gggtggtggg ggcggagttg tagacccatt aacattgttg gcgctcctta gatgggtgtg
     1801 ttctggtgga cctggcaata cccgtgcccg cagcgctcgc gaacagaagc agcacgcccc
     1861 gccggccagt atcgccacct tgccgggagt gatagaatag aggcggcaca cggtagaagg
     1921 gccacgacct caatacgcat gtgtcgcacg ttaagtgcgt tttgaggtcg tggggcctgg
     1981 tagcaaagcc aggcaagact gacgaacgtc cctgaacaaa gggcggctag gctggtgccc
     2041 tgcaaggcgt gggcacgcgg ctcaagcacg gcggttgcca gaggtgtatg tgactggagg
     2101 aggcaccttc cggccacagg cagatagtct tatgcggcct tgaggggctg ggcttgcgca
     2161 cgctcgagga attgcaccgc ctgggcgagg gcgtggttgt tgtcgctgcc tccccaagcg
     2221 aacagttcgc cgcccgcgcc gaggccctgg gcgcgacgct catacggggc gattacaccc
     2281 aggagtcggt gctgaagagc gcgggcgtgg agagcgcgga tgccatcgtg ctcaccgata
     2341 gcagcgatct cggcaatttg cacgcggccc tcacagccca ggaactcaac ccccgcatac
     2401 gcgtggtgct gcgcatgttc aacgacgagt tgggcaaacg catacaggcc ctcttctacg
     2461 attgccacgc cttcagcgcc tccgccattg ccgccccgtc gttcgtctcc gccgccctct
     2521 accaggactg ggagcagcag atatacctgg aggggcgcat gctcgtcgtg caccaggcgt
     2581 ccacggccga cacgaatgtt gtgctgcccc tggcctgcgt ggagagcgac ggcacaacca
     2641 ctcttttccc ggaagaccag cacgaggcga tttgcctggt cgatagcggc ctccggccgg
     2701 acgaggttgg cgaggggcct cactctaaag accgcgcgcc caggggcagg ttggcacggc
     2761 gcgtgcgcag gctgggtacg gctttcgggg cgctgggcgc tatggcggac cgcaaattcg
     2821 cttacctgct gctggccctt acgctcctgg cgtgcctggg cacgattgtt ttctattcct
     2881 tcgcccgcct agacttcgta caagccttct acttcaccgt gactatcctc atcggcaccg
     2941 gcttcgacca gatcacgatc cccgacgcgc ccctggccct cgagttcttt ggcgtgttcg
     3001 tggtgctgct gggagccgtg gccctaacgc tgttctacac cttcatcacc gatgcgttag
     3061 tgggggcgcg cctggcccgc ctgctcgggg cggcgcacgg ccggatgaag ggccacgtag
     3121 tggtgtgcgg agtgggcagc atcggttaca gggtcgtgga gcagatagcg cggcagggca
     3181 taccggtggt cgcggccgag ttgcacgaga acgactttat ccacaccatc aggcgactcg
     3241 gggtgcccgt ggtgatggcc gacgtgcggc tgaaggagac gttggaggaa cttgacgtgg
     3301 gcgaagcccg gtgcctggtc gccgccaccg acgatgacct ggctaacctt caagccgccc
     3361 tcaatgcacg tgctatcaac cccacgctgc gcgtcgtatt gcgcgtgttc gaccccgacc
     3421 tcgcggcacg ggtggagcgc gcttttagca ttcgtgtctc gcggagcgtg tcgtcgctgg
     3481 cggcccctac tttcgccgcg gcggccgttg gaggcaaggt gatagccacc gcggcggtgg
     3541 gcaagcgtgt gctgatagta gggcagggta ggatcgagag ggggtcatgg gcggagggta
     3601 agaacctcga acagttgcag agggcaatag gaggacgcgc catactgttg tcggctgacg
     3661 ggcacaagac gtggcgaccc gaccgcgcga ccgtgctgtc gaaggggcaa gaactggtgg
     3721 tagcgaccac gcgcgcaggc tttgcccggt ttgtcgagag cgttgaaggg catgagcgat
     3781 ctcaagaata acctgtcgaa cataagcaaa taagctccgg caatgtcatt tcgagcgtag
     3841 cgagaaatct cgtcctccgg cacccaagta ccacttttct tccgggcaca gtctcatgag
     3901 aaaagggaaa catcctggtg aaggacgaga tttcccgcta cgctcgaaat gacattgcat
     3961 gaagtacagg catggcatgt gctgtagacg agaacggcag aaacccagga acagcttccc
     4021 gcagtagcga caccagtaga ggagcaaccc aatgcggcac ttgttgatcg acaacgtcag
     4081 agtgtgggac ggcacgggcg caaccacgca agccgggcaa gcggtggagg tgcaagacgg
     4141 gcgcatcgcc tgggtgggtc ctgcttccga ctggccgggc cgccgcgccg gtgtcgccat
     4201 cgtggacggc cgggcgcgca cgttgatgcc cggcctgata gactgccacg ttcactacgc
     4261 cagccccggc ggccccgact ggatagagcg cttcaccgac cccgagccgg aagtcacgct
     4321 ccgcgccatc gacctggcgg gggcttcctt gcgtagcggg gtcaccagcg cccgcgacgt
     4381 gggtgcgccc gatgacctga acatccgcct ctcgaaagca gcggccagcg gggcgttccc
     4441 ctcgccccac atccacgctg ccgggacgtg gatagcccac cagggcacgt acgtctcatt
     4501 cgcccgccaa ttcgctaccg cggacgagtt gcgccaggct atcagcgtcg aagtcgaggc
     4561 gggggccgac ctcatcaagg tagccctcgc accctggaac gaaggcaaac gcccggaggg
     4621 tgcccccgct gtaccgttcg acgccgacct gctggcggta gcggtggagg cggcgcacgc
     4681 tgccgggctg agtatcgcct gccacgccaa cgacccggag agttgcaagc tggcagccag
     4741 ggctggcgta gactcattgg agcatggcat gcacctggat ggcgacgacc tggccgccat
     4801 ggcaacccac gggaccgtgc tcgtgcccac gctttccgtc tgggactcct ggctgttcta
     4861 cacgcgggag gtgggctggc cggaggcacg gaaggcgcgt gccgagtcgc tgcgcgaaag
     4921 ctcgcgggcg gcggtgtcgg gggcggcaaa ggcgggagtg ccggtggccc tgggcacaga
     4981 cgccggaggt ggctcggtgc gccacggccg ggtggcccgc gaggtggagc taatgatcga
     5041 gtgcgggctg agtcccgagg cggccctgca agcaggtacg accgtggccg cgcgcctgct
     5101 gggcgtgtcc gaccggggcg ctatcgaggt tgggcaggtc gcggacatgg tgctactcga
     5161 cgcaaacccg ctggacgacc cggctgcgct ccgcctggtg gccgcggtgt tccagggcgg
     5221 gcggcgcgtc gcgtaagcac acggcggagg caaattgcta gttgagcata cagtcctcat
     5281 ctgccggcat catatgtcat ttggaggtat agtgtactca tatccaacag cagtaagctg
     5341 cccaacgttg atatcccaag gataaattat tcgcatcgtt ccgccagtcg ctatgggcag
     5401 ctggtcagca tttctagtca cgataatctc agatacaatg catagagcta cattgctgta
     5461 ggttcgagca tgaatgacct cattaggagt aagtagcact cccgatcctc ttgatgttgt
     5521 tccctttact tcaacatgta gttcgtcagt atcgtccaga gtcggctgtg tgcagcgtag
     5581 gtcatagctc atggttgccg aaacatcctc tacatgccag ccccttgcta tatagtactc
     5641 aatagcaaag ttcatcgcat gcaattctat agcacgtctc gtcccagggt ctacactgta
     5701 gccttggcca gaggatttcc gccccgctct ttgagcaatg tactgatgca cttctacaag
     5761 ttcaggtatg ggcgtgtgat aatcctcttc atcgataggt tgctcaattg cgcctaactg
     5821 tctccgaata cgtgctacct cgcgtgctaa tccttcgggg ttacttataa actcctccca
     5881 aacgtcccta tcaccttgcc ctcccctata cagcgcacgt ccggggtgat ttgggtcaat
     5941 actcgcaaaa ttggataatt tcatcgatac gccattaggg ttacgaaaac gttcagcgtc
     6001 tgggtgcgta ggatttcgag acaactgatt tagggcttga cttagttcaa gcacatgctc
     6061 gctcctgtta tctaacattc cctcacggag atacaggtcc agagctaata tcaactcatc
     6121 ccgcgtccaa ttagggttcc taggcatgtt tttactcccg atactatata gctactgtat
     6181 ggctactgta accaataact tggggacggt acttctccaa ttgcactttc cttgtttagg
     6241 cattgttctt cgtgaacgcg gcgagtatag aatgagtgta actgtttgtc aactgctact
     6301 gatgtgcatt tgttggatag ctgtaccatc tcacgctaga gtgtcacttc ccttgccggg
     6361 attgctgcgg ctgatagaat agatacggca cacctggtag catctatgta atatatttca
     6421 ggtgtggaga gttaagcgtc actcacggca gcattgtgga ggaggtcgga cagcatggcg
     6481 gcagaagttg gtccgggcgc agaatacgtg atccccatgg agcggctgga gtccttttac
     6541 gacgagccgg gagagtacgg ctggatcatg gaaggccaca aacatggctt cggtctctct
     6601 tcgatcataa tcacgcagac agccccacgg ggtggccctc ccctgcacgt ccatgacacg
     6661 gaggaggtgc acgtactgcc tgaatgccac ctggcgtacg tgatcggaga gagcaccttt
     6721 gaggtcgagg ggccgtgcgt cgtgaacatt cccgctcgcg tgcctcacac cttcctcaac
     6781 atcggcccgg cggtcgcccg tatcgtgtgc ttctggcccg ccaacgactt ctggaccaac
     6841 tacgacgagc taggccctaa ccctctgctg gcgcgctatg gagccggagg acaggctgtc
     6901 tgaacgggcg ttttgtagca agggaagccc tgcatggaaa tagagtccct atcctgcaat
     6961 aactgcggcg cacccctgga tgtgccgtct acagctaact tccttacctg ccgctactgc
     7021 ggctcgaggt tagttgttcg ccgtaccgaa accgcccact ataccgagct aatggagcag
     7081 ttggagcggt ccacacagca actagaggac caggcagaat acatgcgcct gcgcgacacg
     7141 gtggacgcgc tcgatgcgga ttggcagcgt gagcgcaggc ggcatctaat ccgcaggcca
     7201 aacgggtcgt tgcagtcccc cacgcgcagg tacttgggca cgagtattgt agtagccctg
     7261 gtagtgtttg gccttaccgg gttgtggttc tatgccagcg cgagcgctgg ccccggcgcc
     7321 tcgtgtttcg gttggattgg ggcagtcatt tcccttgcgg ctgtagcaca cgcagcgtgg
     7381 gtcagcaata aggtcagcaa ctacaacctg gcaaagcggg tgtaccacca ggagcgcggc
     7441 gagctattga aaaggatgta cgaggtctac ccccaggagc ttgactgggt gagggagtga
     7501 cccgcagccg acgtggaacg cctgaccatc gatagaaagg aacaaccaca tgaccggcac
     7561 caacgaccat atcgactcgt tcctgcaaga gcgcctcgac cgctacatcg atgagacggc
     7621 ccgcctgtgc gcccaaccga gcgtgtcggc caccggagag ggtgtggccg agtgctccaa
     7681 catcgtcgcg gagatactgg cgggccacgg tttcgaggtg cagaagttcg acacgcccgg
     7741 cgagcctgtg gttgtgggcc gcgcccgtgg caggtccgag cgcacgctgc tttgctacaa
     7801 ccactacgac gtacagcccg ccgagccgtt cgagctttgg acctcgcccc cgttcgagcc
     7861 gaccgtgcgc gacggtgccc tctacgctcg cggggccaag gacgacaagg gggagttcat
     7921 cgctcgcctg gccgccgtcg atgcggtgcg ggccgcgcat ggcggggagc taccctgcgg
     7981 ggtcacgttc gtggtggagg ggcaggagga gataggcagc ccccatatcg cccggttcgt
     8041 gctcgaccac ctggacctgc tcaagtcgca cggcgcgatc tgggaggagg gcggcgtggg
     8101 gccggaggag aggccgttca acgtgctcgg ggccaggggc gtgctcacgg tcgagttcca
     8161 cgtacgcacc ctctcccgcg acgctcactc aggccagggg tcggtgctgc ccaacgcggc
     8221 ctggcgcttg ttgtgggcgc tcaccagcct caaaggcccc gacgggcgca tccgcatacc
     8281 cggcttctac gaccaggccc tccccgcctc ccaactcgac aaggacctgt tcgccgccct
     8341 cccctccggc gagcatgagg cgcgggagac gttcggcgtg aaggagttcc tgcacggggt
     8401 aacgggcgag gcgtacaagc tggcggtgtt cgagccgacc tgcaacgtgc aaggcatcgg
     8461 cagcggctgg cagggggtag ggcctaagac gatagtgccc gccgaggcca gcgccaaggt
     8521 cgatttccga ctcgtgcccg accaggatcc cgacgacatt ctggagaagt tgcggcggca
     8581 cctggagagc gaaggcttcg gggacgtgga ggtcacacgc ctgggtgcga tgtggcccgc
     8641 caagtccgcc cccgacgacc cattggtgct gctcaccaac cgcgccgccg aggaggtgta
     8701 cggcgtgccc agcctggtga tgcccatctc cggcgggagc agcccgatct acgcgttcgc
     8761 cggaccgctc ggcatcccgg tcgtcacggc gggcgtgggc tatctcggct cgcgcaccca
     8821 ctcgcccgac gagcacgtcc gcctcgacca ctttctcaac ggcgcgaggc acatcgcccg
     8881 catcgtggac ggcttcgcgg agatttagag agtaacgagt gatgaggact gaggactgag
     8941 gactgagtaa cgcgtaaagc ggctccaggg accatgtacc ctgagagccg ctttgttgct
     9001 tattgcacca ttacgtgtta ctcaatactc gttactcagt cctcagtcct cattactcgt
     9061 tactcgttac gtgtgaggaa cgcgtccacc acatcggggt cgaagtgggt gcctgctccc
     9121 atgcggatgt gctcgcgtac cttctcaggc ggccaggcgg cgcggtaggg cctatcggag
     9181 cttagagcat cgtgcacgtc caccacggcg aagatacggg cggcgagggg tatctgctct
     9241 cccttgaggc cacgagggta gccggtgccg tcccacttct catggtggca gtagggtatg
     9301 tccagggcgg ggcgcaggaa cgagataggc gacagcagct cgtaagcgta tacggggtgt
     9361 cggcgcatga tctcccactc ctcatcggta agtggccccg gcttgaggag gatggagtct
     9421 ggaatgccca tcttgccgat gtcgtgcagc agcgcgccac gctggatttg cagcactccg
     9481 gcttcaccca cgcccattga gcgcgccagg cgcaccgtca cgtcggtcac gcgccgggtg
     9541 tggccttcgg tctccttgtc gcgcaggtcg agggcacgcg accagccctc aagcgtgtta
     9601 tcgtaggcca gggccagctc cgtgttggag cgttgcagtt cctggaacag ggtggcattg
     9661 tcaatcgcga tggccgcctg cctggccagg gcgtccgaga actgtagcca ggcggcatcg
     9721 gggtccagcg gggagcgatg cactatctcc aacacgccct gcaccttacc cctggcaatg
     9781 agcggcaccc cgtggtacat gaggaagcct tccctctcgg ctaccgagaa gagcatgggc
     9841 tgctttcgat tcagcatgtt tgggatatgg atggtatggc gttcgagcgc ggcccgcccg
     9901 gcgaagcctt cacccaggcg catgcgggtc ttttgaacgt cgctatgcct gaagccgcgc
     9961 acggcgacgc attccagcga ctgggtatgc gggttgagca gcagcacgtc agccgcgtcc
    10021 acacccagtt gcgacgtaac ctgctccagt atgacgtcca gggtgacggg caggtcgagg
    10081 ctggcggtga tgaccttgtc aatgctgtgc atggcggcca ggtggtcgaa ctggcgctgt
    10141 agacgttgct cccgctcgtt gcgctctgtg atgtctctcg agatacccgc gacggctata
    10201 ggcgcgccat tgccgtcgcg gatcacagtg tctatcacgt aggccgggaa ggtggtgccg
    10261 ttcttgtgct gtagggtgaa ctcaccttcc cagtagcctt ccctgaacac ctgctccatg
    10321 acctgcttcg ccttaggctg gtcgggaggc atgatgagca attcgcgcag ttccttgccc
    10381 agcacctctt cctgcttcca gccatacagt ttctcggcat aagcgttcca atgcgtcacc
    10441 cgccgctcca tgttcatgac gacgatcccg gcctgtatct ggtcgagcac ctgggtctgg
    10501 atgcgaatct gctcttcggc ggcgcgccgc tccgtcatgt cgctgaacgt gacgaccacc
    10561 gcgtagggga cggtctcgcc gggattgaaa agtgggtgcg agttgatgct gatccaggca
    10621 agcgtgtcgt ccggcttgcg cacgcccatt gggacgttag tgaggaatcc tccggttcgc
    10681 aagctgacca tcgcagggtg gtcctcccca ggaaatggcg agccgtcttc gcgtacggcc
    10741 cgccagcacg gatctatgga gctacgccct atcaactggt cccgtttgag gccgagcacc
    10801 tgttccgccc tctcgttgca ttctacgatg cagcccgaag catcctggac caccaggcct
    10861 tcctgcagag acatgacgat ggagcggaaa tgacgctctt tagcttcgag ggccttctct
    10921 gcccgtatgc gggccgtcat gtccctggct atcgcagacg cgccaacaat agcgcccgct
    10981 tcatccagga tcgaggagac gctgacagac accgtaatgt ggtcgcctgt cttggtcagc
    11041 cttaccgttt cataagcctc gacgcgctcc cccttgcgga tgagacccag gattgagttg
    11101 acctcgctgt gccgctcgac gggtactagc acgtaaatgg acctaccgac catctcttcc
    11161 gccgtgtagc cgtacatctt ctccgcgcca cggttccaac tggtaatgac gccatccagc
    11221 gttttcgcga gtatggcgtc ctcagacgac tcgacgatgg cggccagccg cagcctctct
    11281 gtctcggcct gcttgcgggc tgttatgtcc ctgaaataga cggaaaggcc gccgccttca
    11341 tgcggttggg cgttgaatgc aaaccaggtc tcgaaaggtt cgtaatatgc ctcgtactcg
    11401 acggcagctt ggatcttcca ggcctctcga taattgactt tgaagcctgc ctggtctacg
    11461 tgcggataag cctcgaatac ccttttgcca agcagc
//
LOCUS       DASRWZ010000198         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1112848, whole genome shotgun sequence.
ACCESSION   DASRWZ010000198 DASRWZ010000000
VERSION     DASRWZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112848"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..392)
                     /locus_tag="VFH60_03040"
     CDS             complement(93..392)
                     /locus_tag="VFH60_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312786.1"
                     /translation="MTDKHAHNSPTGKATNARRSPMTHNRDSTASSAGDTASDDLVTL
                     EVTVRREHIALLRRFATREGASVEAMASLWLEEKADEAMRASRSSSGGQASGGGE"
     gene            complement(389..844)
                     /locus_tag="VFH60_03045"
     CDS             complement(389..844)
                     /locus_tag="VFH60_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312787.1"
                     /translation="MLLALGVLIGWAVAAFSPAGLSAQQRAGGDIVMISGRDDHGLLQ
                     DSRIKLQRAPNDEAAVGFAANGSFARVVEQRGEWLKVQLVEDAAGLGWVNDFYLRGRM
                     IRTDGGGQVDLADARVVEGKLFIGVRPVAEPTAELVWIDSSALQEIGAE"
     gene            complement(964..2925)
                     /locus_tag="VFH60_03050"
     CDS             complement(964..2925)
                     /locus_tag="VFH60_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET6312788.1"
                     /translation="MDGAFDGRGSRCRRVVAHVPVPAQGAEKGKAGGQSTRNFRSPAR
                     RVQRGTILKSLFGLMLLVPLFAVGLLGPSRAGATGTAVPCSTNGTWTQGEVNLYWLDV
                     EQGDSQFIVGPTGKTLLIDLGETSFNTYGSTTKATSVANKIKTICGTGTDPVALDYVM
                     ISHHHLDHIGYARNPTDTANYGNGLYQLLTPANLNFTVGTFIDRDSGTWTDTNNNGVC
                     EVGTSTNPSNEIAWHNAGTTSQTARRFICWLYGPSGQADRANIEGHVTTLTNNSTWPT
                     IDLGTGVNVTILNANGKDTMQADGVTPVSGDHTTQGGNGPPSENDYSVAVKITYGNWK
                     YATAGDTDGEYNTSSFGYTYNNIETKIGPLFGNVDTMRANHHGSDHSSSPAYIDTLKP
                     ETVFISCGSNSYGHPGNRMMDDLRDVSNDAGTGADIYLANNPCDQYQSDGTTLTDYTG
                     ALNSNGDVVLRTTNSGTGYTIYYDTGSRSYTAYAYTPTPTPTNTPTNTPTPTNTPVNT
                     PTATPAGPSSVVINEYLMAPNVTYATEFIELYNPANNSINIGGLYLDDLAGGGGSPKL
                     IPANTTISAHGYYVMEIASGFLNNTGSDSVRFLSIVGGVETVYDSTSYSLGSTQYDKG
                     YHRNGDGGSWCGTISTNVTKGTANQSTCP"
BASE COUNT          527 a          888 c         1052 g          670 t
ORIGIN      
        1 gaggtagggg cagcatctta gattcttcgc tacgctcaga atgacagagg ccgcctttgt
       61 tcgtttgctt cctatgcaga agagaccggg tgctactcgc ccccaccact ggcctgaccg
      121 ccagatgagg agcgagaggc gcgcattgcc tcgtccgcct tctcctcaag ccagaggctc
      181 gccatcgcct ccacagaagc gccctcgcgg gtggcaaagc ggcgaagaag ggcgatgtgc
      241 tcgcgacgaa ccgttacctc cagggttacg aggtcgtctg aggcggtatc gccggcggaa
      301 gatgctgtgg agtcccggtt gtgggtcatg ggcgacctcc tggcgtttgt ggctttgcct
      361 gtgggtgagt tgtgcgcgtg cttatctgtc attcggcgcc tatctcttgt agcgcggagg
      421 agtcgatcca cacaagctct gctgtaggct cggccaccgg gcgcacgccg ataaacagct
      481 tgccttctac tacacgagcg tccgccaggt caacttgccc gccgccatcg gtgcgaatca
      541 tgcggccgcg caggtagaag tcgtttaccc agcccaggcc tgcggcgtct tcgaccagtt
      601 gcacctttag ccactcgccg cgctgctcga ccacgcgggc aaagctgccg ttagccgcaa
      661 agcccacggc cgcctcgtcg ttgggagcgc gctggagctt gatcctggag tcctgtagca
      721 gcccgtggtc gtcccggccg ctgatcatga cgatatcccc gccggcccgc tgttgtgcag
      781 atagcccggc gggggagaat gcggccactg cccagccgat caagacgcca agcgccagca
      841 gcaccacccc cgccgcgacc agacgtggag ccccagcaga cggaaaccag tggaacctac
      901 gttgaggctc cactggtttc gcgagttccg agttctttac ggaccttaca ggcttcacca
      961 tgcttatggg caggtggact ggttagcggt gcccttggtc acgttggtag agatggtccc
     1021 gcaccagctc ccgccatcgc cgttgcgatg atagcccttg tcgtactgcg tgctgcccag
     1081 gctatacgag gtcgagtcgt agaccgtctc taccccgccc acgatgctca agaagcgcac
     1141 cgagtcggac ccggtattgt tgaggaagcc gctggcgatt tccatcacgt agtagccgtg
     1201 cgccgagata gtggtattgg cgggtatcag cttgggcgag ccgccaccgc ccgccaggtc
     1261 gtccaggtac aggccgccta tgttgatcga gttgttggcc gggttgtaca gctctatgaa
     1321 ctcggtcgcg taggtgacgt tcggggccat caggtactcg ttgataacca ccgaggaagg
     1381 gcccgccggg gtcgcggtcg gcgtgttgac gggtgtgtta gtaggcgtag gcgtgttggt
     1441 cggggtattg gtaggcgtgg gggtgggagt gtaggcgtac gcggtgtagg agcgcgaacc
     1501 ggtatcgtag tagatcgtgt agccggtgcc ggagttagtt gtgcggagca ctacgtcgcc
     1561 gttggagttc agcgcgccgg tgtagtcggt cagcgtcgtg ccgtcggact ggtactggtc
     1621 gcaggggttg ttagccaggt agatgtcggc ccccgtgcct gcgtcgttcg acacgtcgcg
     1681 gaggtcgtcc atcatgcggt tgccggggtg gccgtagctg ttgctgccgc aggagatgaa
     1741 gacggtctca ggcttgagcg tgtctatgta tgccgggctg gaggagtggt cgctgccgtg
     1801 atggttggcg cgcatggtat cgacgttgcc gaacagcggc ccaatcttgg tctcgatgtt
     1861 gttgtaggtg tagccgaagg agctggtgtt gtactcgccg tccgtgtcac cggccgtggc
     1921 atacttccag ttgccgtagg tgatcttcac ggctaccgag tagtcgttct cggagggcgg
     1981 gccattgcca ccctgcgtgg tgtgatcgcc actgacgggg gttacgccgt ccgcctgcat
     2041 cgtgtccttg ccgttggcgt tgaggatggt cacgttcacc ccggtaccca ggtcaatcgt
     2101 gggccaggtg gagttgttgg tcagggtggt tacgtggcct tcgatgttgg cgcggtcggc
     2161 ctgacctgat ggcccgtaca gccagcagat aaagcgtcga gcggtctgcg aggtcgtgcc
     2221 cgcgttgtgc caggcaatct cgttcgaggg gttggtggag gtgccgacct cgcacacgcc
     2281 gttgttgttg gtatcggtcc aggtgccgct atcgcggtcg atgaacgtac cgacggtaaa
     2341 gttgaggttg gcgggggtga gcaactggta aagcccgttg ccgtagtttg cggtatcggt
     2401 gggattgcgg gcgtagccga tgtggtcgag gtgatggtgg gaaatcatca cgtagtcgag
     2461 agcgacgggg tcggtgccgg tgccgcagat ggtcttaatc ttgttggcga ccgaggtagc
     2521 ctttgtggtg gagccgtaag tgttgaacga agtctcaccg aggtcgatca agagcgtctt
     2581 gccggtgggg ccgacgatga actggctgtc gccctgctcc acgtcgagcc agtagaggtt
     2641 cacctcgccc tgtgtccacg tgccgtttgt cgagcagggc accgcggtac ccgtagcacc
     2701 ggctcgcgag gggccaagca gaccaaccgc gaagaggggg actagcaaca tgaggccgaa
     2761 cagcgatttc agtatggtgc ctctctgcac tctgcgtgcg gggcttcgga aattgcgcgt
     2821 actttgcccg ccagctttgc ccttttcggc accctgcgcg ggcaccggca catgggctac
     2881 cacccgccta cacctagacc ctctcccatc gaacgctccg tccatgtatg tactccttta
     2941 gctgccgggc caaacggtcg aggcgcgggc agtatacgag ggtgggccgc gtagcaacaa
     3001 tgctgcctta cacgcacagc attgtaacgc gacgacgacg tggcaacaga gcaccacgtt
     3061 aggcaaaggc gctgcatcat cggtaagttc tagccttatg tccgagttta gtataccatt
     3121 aatttgaccg aaaatgg
//
LOCUS       DASRWZ010000199         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_564916, whole genome shotgun sequence.
ACCESSION   DASRWZ010000199 DASRWZ010000000
VERSION     DASRWZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564916"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VFH60_03055"
     CDS             <1..777
                     /locus_tag="VFH60_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET6312789.1"
                     /translation="VRSYEGDAWGYLSSAWLLRYLEHSAVMAAADVGYGSRFHEENRS
                     AWVIRRMTLLMPRLISLGQDLEIVTWISHFARVRGGREYRVFDAGGELVATGIAEWVY
                     MDRATLTPMAIPQQTAIDFDVPGAPLGNYDPPPLEPPDEVEGWEHSTSRVAYWHECDS
                     LGHVNNAVYTGWLDDAMFAAVEAHGTSVGAQREDGLRLGGVYYKLDYKRAALPGDELT
                     VTTHLERQSGPSYAVSQRIISADGTALLTASSIYTWQSVL"
     gene            complement(844..2670)
                     /locus_tag="VFH60_03060"
     CDS             complement(844..2670)
                     /locus_tag="VFH60_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312790.1"
                     /translation="MSRLKQLGILAAITFLLVIALLSTVLGARAQPAPTAQPTKLVWA
                     QVFDPTVAEYDLQSVFMTGPNDAWAVGGRYGKGGIIVRYEFINGVWRSVSSKPYQAPL
                     NAVVAVGDRELWAVGDVETIIRITTDGRNVVSIEEMGDRIPLPSARPRADLLTIQMLG
                     NGEEGWVGGYWTIASPGAAPYPMLLHYKQGVWEVDNSTLGSGAIHSLHLTQGGGWMVG
                     QNGIWRFEGGNDSMTGAWAQEQKPPACPDTVCYETLTGVRAISADEAWAVGSRSGTCA
                     ICVSAPYILHRTGGKWQRVVPDKPVANLNLPPQTASFLSNVYFTDKDSGLAVGHYRDV
                     LNATGPGDIRRPLVFSYRSGAWGNEPLPGASRGELADVSMADATHALAVGSDGLVLSY
                     GYGSQPAPSPTPGLTPQPQPTQYLTAPVPDPQDPGVSYFPLVGHTLRGAFRDYWQGHG
                     GLPQFGYPITEEFREVSPTDGRAYAVQYFERARFEYHPENVPPYNVLLGLLGRTVTAG
                     REHEQPFVPAQPSGAPGTLYFKETGHNMAQEFSSYWREHGGLPVYGYPISEPFPELSR
                     TDGKTYLVQYFERNRLEWHPELPDPYKVSLGLLGVQVVYGRK"
BASE COUNT          536 a          993 c          966 g          522 t
ORIGIN      
        1 gtgcgcagtt acgaaggcga tgcctggggg tatctgtcct ctgcgtggct gctgcgctac
       61 ctggagcact cggcagtgat ggcagccgcc gacgtgggct acggctcccg gttccacgag
      121 gagaaccgtt cagcctgggt catacggcgg atgaccctgc taatgccacg cctcataagc
      181 ctggggcagg accttgagat cgtcacctgg atatcccact tcgcacgcgt gcgcggcggc
      241 cgcgagtacc gggtgttcga cgctgggggc gagctggtgg ccaccggcat cgcggagtgg
      301 gtgtacatgg accgcgccac cctcaccccc atggctatcc cacagcagac cgccatcgac
      361 ttcgacgtgc ccggtgcgcc gctcggcaac tacgacccgc ccccgctcga acctcccgac
      421 gaggtggaag gctgggagca cagcacgagc cgggtcgctt actggcacga atgcgactcg
      481 ctaggacacg taaacaacgc cgtctacaca gggtggctgg acgacgccat gttcgcggcg
      541 gtagaggccc acggtacgag tgtaggggca cagcgcgagg acggcctacg gcttggcggc
      601 gtgtactaca agctcgacta caagcgcgcg gccctgcccg gcgacgaact gaccgtcacc
      661 acccacctgg aacggcagtc cggccccagc tacgccgtgt cccagcgcat catatccgcc
      721 gacggcacag ccctgctcac cgccagcagc atctacacct ggcaaagcgt gctgtaagcg
      781 cgctgccgta ccacccaggg cgtccccacg gggcgtcccc tcgctgccga gcatgacggc
      841 accctacttc cggccgtaca caacctgcac gccgagcaac cccaggctca ccttgtacgg
      901 gtcgggcagt tctgggtgcc actccaaccg gttgcgctcg aagtactgca ccaggtatgt
      961 cttgccgtcc gtgcgcgaca actcagggaa cggctcgcta atcgggtagc cgtagaccgg
     1021 caggccgccg tgctcccgcc agtacgacga gaactcctgc gccatgttgt gccccgtctc
     1081 cttgaaatag agggtgccag gcgcgccgga gggttgcgcc ggcacgaacg gctgctcgtg
     1141 ctcgcgtccc gccgtgacgg tgcgcccaag caggcccagc agcacgttgt acggcggcac
     1201 gttctccggg tggtactcga agcgggcgcg ctcgaagtac tggactgcgt acgccctgcc
     1261 atcggtgggg cttacctctc ggaactcctc ggttatgggg taaccgaact gcggaaggcc
     1321 gccgtgtccc tgccagtagt cgcgaaacgc cccgcgcaac gtatgcccga ccagtggaaa
     1381 gtagctcacg ccggggtcct gggggtcggg cacaggtgcg gtaaggtact gcgtgggctg
     1441 cggctgcggg gtaaggccgg gggtggggct gggcgcgggt tggctgccgt agccgtagct
     1501 caagaccagg ccgtccgagc ctaccgccag cgcatgcgtc gcatcggcca tgctcacatc
     1561 cgccaactcg cccctggagg cacccggtag aggctcgttg ccccacgccc ccgacctgta
     1621 gctgaacacc agcggcctgc gaatgtcgcc aggccctgtc gcgttgagca cgtccctgta
     1681 atgccccacc gccaggccgg agtctttatc tgtgaagtag acgttgctca ggaaagaggc
     1741 ggtctgcgga gggagattga gattggcgac cggcttgtcg ggcaccaccc gttgccattt
     1801 gccccccgtg cggtgcaata tgtaaggcgc ggagacgcag atagcgcagg tgccgctacg
     1861 gctgcccacc gcccatgcct cgtcggcgct gatagcccgc accccggtga gcgtctcata
     1921 gcacacggta tcggggcagg cgggcggctt ctgttcctgc gcccatgcgc cggtcatgct
     1981 gtcgttgccc ccctcaaaac gccagatgcc gttctgcccc accatccagc cgccgccttg
     2041 cgtcaggtgc aggctgtgaa tggccccgct tcccagggtg gagttgtcca cctcccacac
     2101 gccctgcttg tagtgcagca gcatcgggta tggcgcggcg ccggggctgg ctatagtcca
     2161 gtaaccaccc acccagcctt cctcgccgtt cccaagcatc tgtatcgtca gcaggtcggc
     2221 cctgggccgc gcactgggca gcggtatccg gtcgcccatc tcctctatgc tcacgacgtt
     2281 gcggccgtcg gtggtgatgc gaatgatcgt ctccacgtcg cccaccgccc aaagctccct
     2341 gtcgcccacg gcgacgaccg cgttcagagg cgcctggtag ggcttgctcg acaccgagcg
     2401 ccaaacgccg ttgatgaact catagcgcac gatgatgccg cccttgccgt agcgcccacc
     2461 caccgcccag gcgtcgttgg ggccggtcat gaagacgctc tggaggtcgt attcagccac
     2521 ggtggggtcg aacacctgcg cccaaacaag cttggtgggc tgggctgtag gggcgggctg
     2581 cgcgcgggca cccagcacgg tgctgagcag ggctatcacc agcaagaagg tgatagcagc
     2641 cagtatgccc agttgtttta gacgtgacat ggcggtactc gtcaggaggt atttgcaggt
     2701 aaaacgaagc gcccacgata aagttccgac gcagagggga taacagatgg catcgtcatt
     2761 gtcggctggg ggtgccctgg ggttgtgccc tgccaataaa ttggcgggct tacaggaggc
     2821 aaagtcccct acggggacta aagaacatga ggctagggcc tatgtgcaaa ctgtaggaag
     2881 taactgacca aagctgcctt tgtcattctg agcgcagcga agaatctcgt cctccaccgt
     2941 gatgttgccc ctttccgcag cacatagtag cacatggcgc gctgcagagt gaagaaaggt
     3001 ggtatttggg tgctgaa
//
LOCUS       DASRWZ010000200         3319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1044210, whole genome shotgun sequence.
ACCESSION   DASRWZ010000200 DASRWZ010000000
VERSION     DASRWZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044210"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..180)
                     /locus_tag="VFH60_03065"
     tRNA            complement(109..180)
                     /locus_tag="VFH60_03065"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Ile,seq:cat)
     gene            complement(258..1040)
                     /locus_tag="VFH60_03070"
     CDS             complement(258..1040)
                     /locus_tag="VFH60_03070"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6312791.1"
                     /translation="MTYNILEGANIRFGDRTELVLDLIRQSEADVVGLCECTGFDEER
                     GSRFKYFRDRLRMGGVLTRAPSGHHIALLYRRGLRTFSRTTLANTMYNGFARIAFESV
                     ELGPVTVVMTHLHPFSSWYRVTEVQQLLGRAQSSDEAIIMGDMNSIAPTDMPLDLASA
                     TPYLTGRFSGPDGEIDTLPIQAMLTRGFVDLGASGVGSTYPTRLERKHKEHGITVRID
                     YILATPGLASRCRSIWAVDDPPAHDASDHLPVVAEFDLTPVG"
     gene            1264..1962
                     /locus_tag="VFH60_03075"
     CDS             1264..1962
                     /locus_tag="VFH60_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6312792.1"
                     /translation="MNRPTLLLLHGAIGARDQFAPLLPLLGTDFDLHSLDFEGHGSNP
                     PRPHPFSIERFKENVLDYLERHSIEHAHVFGYSMGGYVACDLARSHPHVVQSIATLGT
                     KFYWDPEVAEREVGMLDPRKIAAKVPHFARALAERHTASGWEYVLEQTRELLRQLGQV
                     GGLRPQDVAGIEPRVRLMLGDRDTTVTLSETAEMYRALPRGELEVLPATPHQFERVPM
                     PRLAYTLAQFFAQE"
     gene            complement(2236..2757)
                     /locus_tag="VFH60_03080"
     CDS             complement(2236..2757)
                     /locus_tag="VFH60_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET6312793.1"
                     /translation="MLLAAVTLDGKIARHAHHFVDWSSKEDKKMFMQTSKRAGVVILG
                     NNTYETFPSPLPGRLHVVLTTNVADKQNVPGEIEFTNRTPAEIVADLEARGYTEAVLT
                     GGAQVNALFLAADMVDEIWLTVEPFIFGLGIDLFRGMPFDLRATLLEVRQLNDGGSVL
                     LRYGLREPTPGAD"
     gene            complement(2763..>3319)
                     /locus_tag="VFH60_03085"
     CDS             complement(2763..>3319)
                     /locus_tag="VFH60_03085"
                     /inference="COORDINATES: protein motif:HMM:NF036773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HET6312794.1"
                     /translation="SPGIFAFEPARVPSRPTIYAHLSNTQWGTNFPQWLEGDFKFRVR
                     LAPHAGDWRTGELWGQTSSLDLNELHMRGERGVGSMPSLVERDTGLLLLSLHPAHVGR
                     GLIARYWDALGTHRKVTLSLPQPVARVWRCDLMERPVEELPLQKRPASDPSHKPVSWG
                     VLHVAPHATVTLLLEFDKPQEAAP"
BASE COUNT          604 a         1080 c         1040 g          595 t
ORIGIN      
        1 tccactacgc aaggcaccgg cgcatttggc atgaagatca gaacaataaa cgaggtcggg
       61 gcgttgaatg ccccgacctc gtttattgtt ctgatcttga aactttagtg ggcccggtag
      121 gattcgaacc tacgaccaag cgattatgag tcgcctgcgc taccgctgcg ccacaggccc
      181 cggccacttt taccgcccaa ttataccaaa ccggcggcga tttgtcactg cttacaaccg
      241 gcacccagta ccgggtgcta acccacaggc gtcaggtcga actcggccac cacaggcagg
      301 tggtcggagg cgtcgtgggc gggcgggtcg tccactgccc agatggagcg gcagcggctt
      361 gcaaggccgg gggtcgccag tatgtaatcg atgcgcaccg tgatgccgtg ctccttgtgc
      421 ttgcgctcca gcctggtcgg gtaggtagac cccacacccg aggcccccag gtccacgaag
      481 ccccgggtga gcatggcctg gatggggagc gtgtctatct cgccgtcggg gccggagaac
      541 cgcccggtca ggtagggggt ggccgacgcg aggtcgagcg gcatgtcggt gggggcgatg
      601 ctgttcatgt cgcccatgat gatcgcctcg tccgacgact gggcgcgccc aagcaactgc
      661 tggacctccg tgacccggta ccagcttgag aacgggtgca ggtgcgtcat caccactgtg
      721 accggcccca gctcgacgct ctcgaacgcg atgcgggcaa agccgttgta catggtgttc
      781 gccagcgtgg tgcggctgaa ggttcgcagg cccctccggt agaggagggc gatgtggtgg
      841 ccgctgggtg ccctggtcag cacgccaccc atgcgcagcc ggtcgcggaa gtacttgaac
      901 cggctgccac gctcctcatc aaagccggtg cactcgcaca gccccaccac gtccgcctcg
      961 gactgccgga tgaggtccag gaccagctcg gtgcggtccc cgaagcggat gttggccccc
     1021 tcaagtatgt tgtaggtcat tactctcaca cgactactcc tgtgctcggc ctcttttcgc
     1081 gccccgtggg cacgaccttt catggtgcct gtatgccgca cctggggagc aagaagcaca
     1141 ccagcgggaa cgtgcccgca gggaaaggtg aagtccgtgg gcggttgaat cccggcgcgg
     1201 cccgcgttac actgtcgcca gcacgcgact gctcttccac tccaatgaag gttgaggacc
     1261 aacatgaaca ggccaacttt acttctgctc cacggggcga ttggcgcgcg cgaccagttc
     1321 gcgcccctgc tgcccctcct gggcacggac ttcgacttgc atagtctcga cttcgagggg
     1381 catggctcca accctccgcg ccctcacccc ttcagcatcg agcgcttcaa ggagaacgtg
     1441 ctcgactacc tggagcggca ttcaatagag catgcccatg tgttcggcta cagcatgggc
     1501 ggctacgtgg cctgcgacct ggcgcgctcg cacccgcacg tcgtgcaaag catagccacc
     1561 ctcggcacga agttctactg ggaccccgaa gtagccgagc gcgaggtggg gatgctagac
     1621 ccgcggaaga tagccgccaa ggtgccccat ttcgcccgtg cgctggccga aagacatacc
     1681 gcttcgggct gggagtatgt gttggaacag acgcgcgagc tactgcgaca gctaggccag
     1741 gtaggcggtc tgcggcccca ggacgtagcc ggtatcgagc cgcgagtgcg cctgatgctc
     1801 ggtgaccgcg acaccacggt gacactgtcc gagaccgccg agatgtaccg ggcgctgcca
     1861 cgcggcgagc tggaggtgct gcccgcaacg ccccaccagt tcgagcgggt gcccatgccg
     1921 agattggctt ataccctcgc ccagttcttt gcccaggagt agaaccgagg actagagcct
     1981 atctgcaagg tgctgcctgt gcgggacacc ctcccccttt gtcattctga gcgcagcgaa
     2041 gaatctcgtc cttcagcacc caaacaccac ctttcttcac ggcggtgcta tgatgctgct
     2101 atattgctat cgaaaagggg aacaccaggg tggaggacga gattcttcgc ggagtttacc
     2161 ctgagcgcag ccgaagggct cagaatgaca agggccacct ttgttcgcgt atttcccact
     2221 gtttgcagag acgccttagt cagcgcccgg cgtcggctca cgcaggccat agcggagcag
     2281 caccgacccg ccatcgttta gctgccgcac ttccagcaga gtagcccgga ggtcgaacgg
     2341 catgccccgg aacaggtcga tgcccaggcc gaagatgaac ggctccacgg tcagccatat
     2401 ctcgtccacc atgtcggccg cgaggaatag agcgttcacc tgcgcgccgc ccgtcagcac
     2461 cgcctcggtg taaccgcgcg cctccaggtc cgctacgatc tcggcaggcg tgcggttggt
     2521 gaactctatc tcgcccggca cgttctgctt gtccgccacg ttggtagtga gcacgacgtg
     2581 caacctgccg ggcagcggcg aggggaacgt ctcatacgtg ttgttgccca ggatgaccac
     2641 gcccgcgcgc ttcgaggtct gcatgaacat cttcttgtcc tccttcgagg accaatcaac
     2701 gaagtggtgc gcgtgccggg cgatcttgcc gtccagcgta acggcggcca gcagcattat
     2761 cttcacgggg cggcctcctg gggcttgtcg aactccaata acagggtcac cgtcgcgtgg
     2821 ggtgcgacgt gcagcacgcc ccacgatacc ggcttgtgcg acgggtcact cgcgggccgc
     2881 ttttgcaaag gcaactcctc cacgggccgc tccatcaagt cgcagcgcca gacgcgggcc
     2941 actggctggg gcaggctcaa cgtgaccttg cggtgggtgc caagcgcgtc ccagtagcgg
     3001 gcgataaggc ccctgccaac atgggcgggg tgcagtgaca gcaagagcag ccccgtatcc
     3061 ctctcaacga gagacggcat cgagcctacg ccccgctccc ctcgcatgtg caactcgttc
     3121 aggtcgaggc tggaggtctg gccccacaac tcgcccgtgc gccagtcgcc cgcgtgcgga
     3181 gccagcctga ccctgaactt gaaatcgccc tcgagccact gcgggaagtt ggtgccccac
     3241 tgcgtgttgg agaggtgcgc gtatatagtc ggtcgcgagg gcaccctggc cggctcgaac
     3301 gcgaatatgc cgggactgc
//
LOCUS       DASRWZ010000201         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_633107, whole genome shotgun sequence.
ACCESSION   DASRWZ010000201 DASRWZ010000000
VERSION     DASRWZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633107"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1469
                     /locus_tag="VFH60_03090"
     CDS             273..1469
                     /locus_tag="VFH60_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6312795.1"
                     /translation="MLPVAEQFKIETILPSERARLVRFCAHLSGCIDAAEDLAQETLM
                     EAWRHRDRLSDPAGHQQWLSAIARNVCMRWRRVQGRHSERMAIPVGMGGEPLNVEDIA
                     DDDFDLEVELERSELADLLDRAMSLLPAETRTALVEKYVEEMPIAEIGARMGLSEGAV
                     AVRLHRGKLALRRLLANELSDEAVAYGLVDVWDVDWRETRIWCPGCGRRRALHSNTLK
                     NRYGRFILHCPDCFPAFPDVPITVSHTDIWNPGLQQVLGGVRSTKAVLSRMMAWVEGY
                     YMPRLSGRAVPCTGCGEMVPVQLEIPERYAPSIKERQGVFLRCERCNYFCAERLHALG
                     MCLLEGRKFWQDHPRMQALPLREVETAGVPTLVASFRSVTDGAGYDVLFVRDTYQLIS
                     THIQSE"
     gene            1674..>2850
                     /locus_tag="VFH60_03095"
     CDS             1674..>2850
                     /locus_tag="VFH60_03095"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6312796.1"
                     /translation="MISLLRQRNFSLLWFGGVVSYAGNWMLFIALPIYVYQLTGSAVA
                     IGTAFIIGTLPRLLFGTVAGVFADRWDRRNTIIAANLLMGVGLLPLALVHSVDTLWIV
                     YAVSLFNATVSTVVGPAENALLPTLVSKEDLVTANSLNALNDSLARLVGPPLGGAIAG
                     LLGLMGVAMADASTFFFAAAMVALIRVDARPKKEGGEEVRVQGVWARFWTDWLNGLRV
                     VKHDRQLAIIFVFTAIAQVGEGVLGVMMAVFVSRVFGGGPEELGLLFGSQAIGGMIGS
                     LFVGNVAKRVAPARLLGLSAVLFGIIDLLIVNYPTFYPSIVPAYVLIALVGIPAVGMI
                     TSMYTLLQVGSPDEYRGRVFGAWGTTATLASLVGMVLASAFGDALGPVTLLNLQGSGY
                     "
BASE COUNT          503 a          848 c          972 g          527 t
ORIGIN      
        1 acaaagggga atgcaggatg acaaagggga atgcaggttg acaggcgcgg ggcctggttc
       61 tctagtccgg caaggcgtat tcaggaaggc aaggcaggac tttcctaaag taggtagcgg
      121 caaaaagcgc agtagggtca cactatttac tctctagtgc ggtcctgctg ctataattgc
      181 ttgaaaatgg cgtaagattc cgccgaaagg cagcgataag cggttaggag tgtggccgcg
      241 cgccatagat tgctacctag ccggagagca ccttgttacc agtagccgag caattcaaga
      301 tagagaccat actgccctcg gagcgggcga ggctggtgcg tttctgcgcc cacctctccg
      361 gctgcatcga tgcagccgaa gacctggccc aggagacgct catggaggcg tggcggcaca
      421 gggacaggct gagcgacccc gccgggcacc aacagtggct ctcggccata gcccgcaacg
      481 tctgcatgcg ctggcggagg gtgcaggggc ggcactcgga gcgcatggct atacccgttg
      541 gcatgggcgg tgagccgttg aacgtagagg acatcgcgga cgacgatttc gacctggagg
      601 tggagcttga gcggagcgaa ctggccgatc tgctcgaccg ggccatgtcg ttgctgcccg
      661 ctgagacccg caccgccctg gtggagaagt acgtcgagga gatgcccatt gccgaaatcg
      721 gggcgcggat gggcctgagc gagggggctg tggcggtgag gctgcaccgg ggcaagctgg
      781 ccctgcgccg cctccttgcc aacgagctga gcgacgaagc ggtggcttac ggcctggtgg
      841 acgtctggga cgtggactgg cgggagaccc gcatctggtg ccccggctgc ggtcggcgtc
      901 gcgccctcca cagcaacacg ctgaagaacc gttatggcag gttcatcctg cactgtcccg
      961 actgcttccc ggccttcccc gacgtgccta tcacggtgtc gcacaccgac atctggaacc
     1021 ccggcctgca acaggtgctg ggcggcgtga ggagcaccaa agcggtcctg tcgcgcatga
     1081 tggcctgggt agagggctac tatatgcccc gcctgtccgg gcgcgcggtg ccctgcacgg
     1141 ggtgcggcga gatggtgccc gtccagttag agatacccga acggtacgcc cccagcatca
     1201 aggagcggca aggggtgttc ctgaggtgcg agcgctgcaa ctacttctgc gcggagcggc
     1261 tgcacgcgct gggcatgtgc ctcctggaag ggcgtaagtt ctggcaggac caccccagga
     1321 tgcaggccct gcccctgcgc gaagtggaga cggcgggcgt gcccaccctg gtcgcgagct
     1381 tccgcagcgt aacggatgga gccgggtacg acgtgctctt tgtgcgtgac acttaccagc
     1441 taatcagcac tcacattcag tctgagtaac acaaccataa cggcaagctt cgacttgctc
     1501 tctaatacca aatctccttg agcgcactgt agcaggaggg ggaacgagag aattggggga
     1561 cacccccata cccccgccgg gggtcacccc ctgcccccat attgcagcgc tcgctaacgg
     1621 ccccggtcta tgcatgacgg gtggcgcttg cctgccctga aacggagtac ctgatgattt
     1681 ctttgctgcg acagcgcaac ttttcgctcc tgtggttcgg cggcgtggtg tcttacgcgg
     1741 gtaactggat gctcttcatc gcgctgccga tctacgtgta ccagcttacc ggctccgccg
     1801 tggctattgg cacggcgttt ataatcggca cgctgccacg ccttctgttc ggcactgtcg
     1861 ccggggtgtt cgccgaccgc tgggaccgcc ggaacacgat aatcgccgcc aacctgctca
     1921 tgggcgtggg tttgctgccg ctcgcgctgg tgcactcagt ggatacgctc tggatcgtgt
     1981 acgccgtctc gctgttcaac gcgaccgtga gcacggtagt ggggcctgcc gagaacgcgc
     2041 tgctgcccac gctcgtcagc aaggaagacc tggtcacggc caactccctc aacgcgctga
     2101 acgacagcct ggcgcgcctg gtggggccgc cgcttggggg cgcgatagcg ggtctgctgg
     2161 gcctcatggg ggtagccatg gcggacgcct ccaccttctt cttcgcggcc gcgatggtgg
     2221 ccctcatcag ggtggacgca cgccccaaaa aggagggcgg ggaggaagtg cgcgtgcagg
     2281 gagtgtgggc caggttctgg accgactggc tgaacgggct gcgggtagtc aagcacgaca
     2341 ggcagttggc gatcatattc gtgttcaccg cgatagcgca ggtgggcgag ggcgtgctgg
     2401 gggtgatgat ggcggtgttc gtgagcaggg tgttcggggg cggccccgaa gagttggggc
     2461 tgctgttcgg ctcgcaggcg ataggcggca tgataggcag cttgttcgtg ggcaacgtcg
     2521 ccaagagggt agccccggcc cgcctgctcg gcttgagcgc cgtcctgttc ggcataatcg
     2581 acctgttgat cgtgaactac ccgaccttct acccatcaat cgtgcccgcc tacgtgctga
     2641 tagccctggt gggcatcccg gcggtgggca tgataacgag catgtacacc ctgctgcaag
     2701 taggctcgcc cgatgagtac cggggccgcg tgttcggtgc ctggggaacg accgcgacgc
     2761 tagcctccct ggtaggcatg gtgcttgcca gcgcattcgg tgacgctctc ggcccggtga
     2821 cactgctcaa cttgcaaggc agcggctacg
//
LOCUS       DASRWZ010000202         4551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_565129, whole genome shotgun sequence.
ACCESSION   DASRWZ010000202 DASRWZ010000000
VERSION     DASRWZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565129"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1041)
                     /locus_tag="VFH60_03100"
     CDS             complement(34..1041)
                     /locus_tag="VFH60_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET6312797.1"
                     /translation="MEATLDRVDISLKLLNSSAGRSGSLPRRGVRYDRLARGAAVAGL
                     GLAGLVFGGFPMFAARRIIYPTWSDEYYRATTGKLEMDLPVRPEYVVFEGRDGKALSG
                     WFVPAPEGSAKPWPVVMLAYGYGGFKEQMAGYSRSIHEGGFATFLFDMSGSGLRKGEP
                     GTFGYKERWQMMDAANYLRTRDDVDPGRIGALGVSMGAAATLLAAAEDPGIKAVVADS
                     SYADLFGMVRPGISAFISPRALFLAPLIVRYAETMLGMKSGEVRHDEAAARLGDRPLF
                     IIHGDCDTLTDPNSATRLYDAASGPKQLWVVNECGHAYAPVLAPDEYKQRVNGFFQRY
                     L"
     gene            complement(1355..2008)
                     /locus_tag="VFH60_03105"
     CDS             complement(1355..2008)
                     /locus_tag="VFH60_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6312798.1"
                     /translation="MIKVLIADDHKMFRQGLRMLFEMESDIKVVGEARDGLEVQELAV
                     ALEPDIILMDINMPGTDGVEATRQVLKRRPEQAVIILTMFREDEHVYQAINAGARGYV
                     LKDADSVDVLRALRSVADGGSVLDAAMTNKVFQQFKQMSEKSEKHNPEGLTNRELEIL
                     ALIANGDSNRVIGEKLFLSEKTIKNYITSIFQKLQTSDRTHAAVYAIQHGLIGQPVN"
     gene            complement(2143..3567)
                     /locus_tag="VFH60_03110"
     CDS             complement(2143..3567)
                     /locus_tag="VFH60_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6312799.1"
                     /translation="MVEALLALRAESAGTWRHLGRVARYAIAFGKGLGLDLDALMTLH
                     CGAMLHDVGKQLVPSEILDKASPLDPDEWYALTKHSMLSGQILKARGIPRGVALLAQS
                     HHEWYNGRGYPLGLEGESIPLVARVLSIADAYEAMSNDRPYRPALSPEEALNELDRGS
                     GTQFDPSLTRRLRPLLEAGMEALVPIRTLRVISDDPALHRALWFAAYPWGWEIAPWPA
                     DWSAACPPELICETELSQSVELTVVDGRCVHRLSQDALSSIEGPILWVDAAEGQTPGV
                     SRPLDLCHLLPFLDADTAWNGSGATQPDRVKVLVADPFKLFRQALVRCFENREDVQVV
                     GAVDSPDAYRRALETAAFDVAVVASDFLEGTRTVRELRPGDSLLAGQGEGHTSREHAD
                     VPLVVLLADEDLEDAARSVERQVPPVGVPGLFVGGKRVSIPRGAPVEVLVEAISLLAE
                     KKAGAQPPGRNGPFFEGQQPQTWS"
     gene            complement(3818..>4551)
                     /locus_tag="VFH60_03115"
     CDS             complement(3818..>4551)
                     /locus_tag="VFH60_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312800.1"
                     /translation="LSGALLLLYKRAILAAGDAFDPRDYSLTGPKDDNPANTERLPRI
                     SVAHLAAIDRFNMLLPRLMEERRRIQAARVRSDREILPLLGRLWEPQFAGRVYADASR
                     QLAAALDLYGLTAGALPNRVLVLASPSDAGAMLLATTLGTEFLVALASIEPGAAHGWS
                     AEEGFTRHSLATTAPLVQALVQQADAVLAFPGAAAALTPGVSTPVAILGAGPAGEFPQ
                     AERFDVLDMEAVRRFCAQPGAKHHE"
BASE COUNT          859 a         1460 c         1408 g          824 t
ORIGIN      
        1 acggttggag gctcctcttc atgcaatttg cggttacaga tagcgctgga agaacccgtt
       61 cacgcgctgc ttgtactcgt cgggggccaa tacgggggcg taggcgtgcc cgcactcgtt
      121 tacgacccaa agctgcttcg gccccgaggc ggcgtcatac aggcgggtgg ccgagttggg
      181 gtcggtgagc gtgtcgcagt cgccgtggat gatgaagaga gggcggtctc ccagcctggc
      241 ggcggcctcg tcgtggcgga cctcgcccga cttcatcccc agcatggttt cggcgtaacg
      301 aacgataagg ggcgcgagga agagggcgcg ggggctaatg aaggcgctga tgccgggccg
      361 caccatacca aagaggtcgg catacgagga gtcggccacc acagctttga tgccggggtc
      421 ctcggcggcc gctagcaggg tagcggccgc ccccatagac actcccagcg ccccgatgcg
      481 gccggggtcc acgtcgtcgc gggtgcggag gtaattggcg gcgtccatca tctgccagcg
      541 ctccttgtag ccgaaagttc ccggctcgcc tttgcgcagg ccgctgccgg acatgtcgaa
      601 caggaacgta gcgaaaccac cctcatgtat agacctggag tagccagcca tctgctcctt
      661 gaagccgccg taaccgtacg ccagcatcac caccggccat ggtttagcac ttccctccgg
      721 cgcgggcacg aaccagccgc tcaacgcctt gccgtcgcgc ccctcgaaca cgacgtactc
      781 cggcctgacc gggaggtcca tctcaagctt gccggtggtg gcccggtagt actcgtcaga
      841 ccaggtcggg tagataatgc gccgggccgc gaacattggg aagccgccga acaccaaacc
      901 cgcaaggccc agtcccgcta cggcggcccc acgagcgagc cggtcgtacc ggactccccg
      961 tcgcggcaat gagccgctac ggcccgcgct gctgttcaac agcttaaggg atatgtctac
     1021 tctgtctaat gtcgcttcca tccaattgct cccgtgcgcc cagtatttct gcttgcccac
     1081 attatacgtc ggacctgtat ggctcagatg ttcccctgat gattataggt gggacctagc
     1141 agccacgtca acgcgccgtc cacggatacg cttcagcggg ctgacattac tatgtccaaa
     1201 catgcaagca ccccattcag gcaagcagcg gagtaaatag ccgggaacgc aaacgcccgc
     1261 tctaccacga gaagcgggcg tccacgataa agtgccaaat tatgcgcgga ctaacaccca
     1321 cacaaatccg aaatcgcaaa ttccaacttc caaatcagtt caccggctgg ccgatcagac
     1381 cgtgttgtat ggcgtaaacg gccgcgtggg tgcggtcgct ggtttgcagt ttctggaaaa
     1441 tagaagtgat atagttcttg atcgtcttct ccgaaaggaa cagcttctca ccgataacgc
     1501 ggttgctgtc gccattggcg atcaaggcga gtatctcaag ctcacggttg gtcagcccct
     1561 ccgggttgtg cttctcggac ttctcggaca tctgcttgaa ctgttgaaac accttgttgg
     1621 tcatcgcggc atcgagcacc gagccaccat cggccacgct acgtagcgcc cgcagcacgt
     1681 ccacgctgtc ggcgtctttg agtacgtagc cgcgcgcccc cgcgttgatg gcctggtaca
     1741 cgtgctcgtc ctcgcggaac atcgtgagga tgataacggc ctgctcgggc cgcctcttca
     1801 gcacctggcg agtggcttcc acaccgtccg tgcctggcat attgatgtcc atcaggatga
     1861 tgtcaggctc cagcgcaacg gccagttcct gcacttccag cccgtcgcgc gcttcaccca
     1921 caactttgat gtcggactcc atctcgaaca acatgcgcaa gccctgcctg aacatcttgt
     1981 ggtcgtcggc tatcagaacc ttaatcatct ttacctctct cgtgctagag cgcaataact
     2041 gggtcaagcg gtaacgtgcg ctttagagca actaacactt gaccttgtgc aatgttacaa
     2101 gatttacacc tcggcggcaa tcaccggata gtcccagagc ttctaggacc aagtctgggg
     2161 ctgctgtccc tcgaaaaatg gcccgttccg gccgggaggc tgcgcgcctg ctttcttctc
     2221 ggccaggagg ctgattgcct cgaccagcac ctcgacgggt gcgcccctgg gtattgagac
     2281 tcgcttgccc cctacgaaca agccgggcac ccccaccggc ggcacctgcc tctcgacgct
     2341 ccgggccgca tcttcgaggt cttcgtccgc cagcagcacc accaggggca catccgcgtg
     2401 ctccctgctc gtgtggccct ccccttgccc ggccagcaag ctgtcacccg gccgtagctc
     2461 tcgcacggtg cgcgtgccct cgagaaagtc gctcgccacc accgccacgt cgaacgcggc
     2521 ggtttcgagg gcgcgacggt aggcatcagg cgaatcgacc gcacccacca cctgcacgtc
     2581 ctcacggttc tcgaagcagc gcaccagcgc ctggcgaaat agcttgaacg ggtcggccac
     2641 cagcacctta acccggtccg gctgggtggc accacttccg ttccaggcgg tatcagcgtc
     2701 gagaaatggc agcaggtggc acaagtcgag gggcctggat acccctggcg tctgcccctc
     2761 cgccgcatcg acccatagga ttgggccttc tatggaactg agagcgtcct gagaaaggcg
     2821 gtgcacgcac cgtccatcga cgaccgttag ctcgacagac tggctgagct ccgtttcgca
     2881 aatgagttcc ggcgggcagg cggcactcca atctgcgggc cagggcgcga tctcccagcc
     2941 ccaggggtag gcggcgaacc acaacgcgcg gtgcagagca gggtcgtcgg agatcacacg
     3001 cagggtccga atgggcacca gcgcctccat accggcctcc aagaggggcc gcaggcggcg
     3061 agtaaggctc gggtcgaact gggtgccgct gcccctgtcc agctcgttga gcgcctcctc
     3121 gggagagagg gccgggcggt agggtcggtc gttggacatc gcctcgtagg catccgcgat
     3181 tgacaggacg cgggcgacca ggggtatcga ctcaccttcc aggccaagcg ggtagccgcg
     3241 gccgttgtac cactcgtgat gagactgggc gaggagggcc acgccacggg ggatgccccg
     3301 cgccttgagg atctgcccgg agagcatcga gtgcttggtc agggcgtacc attcgtccgg
     3361 gtcgaggggg ctggctttgt caaggatttc gctgggcacc agttgcttgc caacgtcgtg
     3421 gagcatcgct ccacagtgca gggtcatcag tgcatccagg tcgaggccca gccccttgcc
     3481 gaaagcgatg gcgtagcggg ccacgcgccc cagatgccgc cacgtcccag ccgattccgc
     3541 tcggagggcc aggagggcct caaccatcgg ctctagaagc cagcaatctc ctatttcttt
     3601 tctcgagaca cccgaacttg tgtgcatggc ataccatccg tcctcgtaca tccgccttcg
     3661 caaaggagcg gtgcgtcagt cagcgcgaca cgtgtcgttt gttttcatga cgaggaacat
     3721 ccaggagaag tataccaggg gccaacagcc gctagaagcc gcgatagtca catttgcgta
     3781 gggaccaaag tcccgaaaat gtctcctgtc aggagggtta ttcgtggtgt ttggctccag
     3841 gctgcgcgca aaaccggcgt acggcttcca tgtcgagaac gtcgaagcgt tctgcctggg
     3901 ggaactcacc ggccggcccg gcacccagaa tagctaccgg agtgctcacg ccgggggtga
     3961 gggcggcggc cgcgccggga aaggccagta cggcgtcggc ttgctgaacg agcgcctgca
     4021 caagcggggc ggtggtagca agtgagtgcc tggtgaagcc ttcctccgcg ctccaaccat
     4081 gcgccgcgcc cggttctatg ctcgccaggg ccacaaggaa ctcagtaccg agcgtggtcg
     4141 ccaacagcat cgccccggca tctgaggggc tagccaggac gagcacgcgg ttggggagcg
     4201 cccccgcggt gaggccgtat aggtcgaggg cggcagctag ctggcgggaa gcgtcggcgt
     4261 aaacgcgccc ggcaaactgc ggttcccaca ggcgtcccaa cagaggcagt atctcacggt
     4321 cagagcgcac gcgagccgct tggatgcgcc gccgctcctc catcaggcgg ggcaggagca
     4381 tgttgaatcg gtcgatcgcg gccaggtgcg ccacgcttat ccgaggcagg cgctcggtat
     4441 tggccgggtt atcgtccttc gggcctgtga gggaatagtc ccgcgggtcg aaagcgtcac
     4501 ctgcggcgag gatggcgcgc ttgtagagca gcaggagcgc ccctgaaagc a
//
LOCUS       DASRWZ010000203         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_565160, whole genome shotgun sequence.
ACCESSION   DASRWZ010000203 DASRWZ010000000
VERSION     DASRWZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565160"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1552
                     /gene="purL"
                     /locus_tag="VFH60_03120"
     CDS             <1..>1552
                     /gene="purL"
                     /locus_tag="VFH60_03120"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET6312801.1"
                     /translation="RLGRQPNMVELGMAGAMWSEHCGYKHSKPLLRLVPTQGERVLQG
                     PGENAGVVDIGDGWAVALKIESHNHPSAVEPVEGAATGVGGIVRDIFTMGGRPIALLN
                     SLRFGPLTDPHTRYLFGGVVAGIGGYGNCIGVPTVGGEIYFDPSYAANPLVNAMCVGL
                     LRKERLMRAAASGLGNPVLLVGADTGRDGIHGATFASDTLGEDSLQDRPAVQVGNPFL
                     EKCLMEACLEVLEIEGLVVGMQDLGAAGLTSSAVECAARAGSGIEIDLAHVPRRERGM
                     NAYEIMLSESQERMLVIVTSGREDEAREVFARWGLHSNVIGHVTGDSMVRVFDGPLLA
                     ADLPAHLLADEVPIRYVTGRPDPQIEHLQHPDWDALLPHEDEHTRYDRALLDVLASPN
                     VASKERVFRTYDHTIGSNTVLQPGQADAAIVRVKGTGKAIALTTDCNSRYCYLDPYAG
                     GAHAVAEAARNLACVGAEPLAVTDCLNFGNPENPHVFYQMEGAVNGIADACNALSIPG
                     VSGNVSLYN"
BASE COUNT          257 a          541 c          518 g          236 t
ORIGIN      
        1 cggctaggca ggcagcctaa catggtagaa ctgggcatgg cgggcgctat gtggagcgaa
       61 cactgcggct acaagcactc caagcccctg ctgcgcctcg tccctacgca gggcgagcgc
      121 gtgctgcagg gtccgggcga gaacgcgggc gtggtggaca tcggcgacgg ctgggcggtg
      181 gccctcaaga tcgagtcgca caaccacccc tcggcggtgg agcctgtgga gggcgcggcc
      241 acgggcgttg gcggcatcgt gcgcgacatc ttcacgatgg gcgggcggcc catagccctg
      301 ctcaactccc tgcgcttcgg ccccctcacc gacccccata cgcgctacct gttcggcggc
      361 gtggtcgcgg gcatcggcgg ctacggcaac tgtatcggcg tgcccaccgt gggcggcgag
      421 atttacttcg acccctccta cgccgccaat cccctcgtga acgctatgtg cgtgggcctg
      481 ctgcgcaaag agcggctgat gcgggcggcg gcctccggcc tgggcaaccc cgtgctgctg
      541 gtgggggccg ataccgggcg cgacggcata cacggcgcga ccttcgcctc cgacacgctg
      601 ggtgaggact cgctgcaaga ccgccccgcc gtgcaggtgg gcaacccctt cctcgaaaag
      661 tgcctcatgg aagcctgcct ggaagtgctc gaaatcgagg ggctggtcgt gggcatgcag
      721 gacctggggg cggcgggcct cacctcctcg gcggtggagt gcgcggcgcg ggcgggtagc
      781 gggatagaga tagacctggc gcacgtaccc cgtcgcgagc gcggcatgaa cgcctatgag
      841 atcatgctgt ccgagtcgca ggagcggatg ctggtgatcg tcacgtcggg gcgcgaggac
      901 gaggcgcggg aggtgttcgc ccgctggggt ctgcactcga acgtcatagg ccacgttacg
      961 ggcgacagca tggtgcgcgt tttcgacggc cccctcctcg ccgctgacct gcccgcccac
     1021 ctgctcgccg acgaggtgcc gatccgctat gtaaccggca ggcccgaccc ccaaatcgag
     1081 catttgcagc accccgactg ggacgccctg ctcccccacg aggacgagca cacccgctac
     1141 gaccgggcgt tgctggacgt gctcgcctcg cccaacgtcg cctccaagga gcgcgtgttc
     1201 cgcacctacg accacaccat aggcagcaac actgtgctgc aacccggcca ggccgacgcc
     1261 gccatcgttc gcgtgaaggg cacaggcaaa gctatcgccc tcaccaccga ctgcaacagc
     1321 cgctactgct acctcgaccc ctatgctggt ggggcgcacg cggtagcaga ggccgcccgc
     1381 aatctcgctt gcgtgggtgc cgaacccctg gccgtgaccg actgcctcaa ctttggcaac
     1441 cccgaaaacc cccacgtctt ctaccagatg gaaggggcgg tcaacggcat cgcggacgcg
     1501 tgcaacgccc tcagtatccc gggggtcagc ggcaacgtgt cgctctacaa cg
//
LOCUS       DASRWZ010000204         9794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_633303, whole genome shotgun sequence.
ACCESSION   DASRWZ010000204 DASRWZ010000000
VERSION     DASRWZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633303"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFH60_03125"
     CDS             complement(<1..457)
                     /locus_tag="VFH60_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312802.1"
                     /translation="MPVFPVLTMLPLSATVIAGAFAIVLLNRYFGGKRRPHELAWAVA
                     FLLFAIGAACQVYADVSGGWNPFLARTYYLTGAILNVGFLALGTMFLLFRGRTAAIAL
                     WVTLALSAYAVFALYTVPIDTRPLADSHFVEWSALFTADRTPRWLAALFS"
     gene            complement(684..2480)
                     /locus_tag="VFH60_03130"
     CDS             complement(684..2480)
                     /locus_tag="VFH60_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6312803.1"
                     /translation="MMVNQDDILDVAVVGGGPSGLYSAWRLLTGKTESKGRGKAKGGR
                     PIKKVTVFELSGRTGGRLLTWRPFPEQPGLNAELGGMRFYQEQGLVWGLINNHFVKQG
                     KLQPPIDFYVTDPNGNNIWYLRENNLKAPDLTNPDKVPYRLDARARYADPPTIINDVI
                     GRMLAANREVIAERLGGPTRPMSWQEWDEVKPYLRYGSRRMWDVGFWNLLWDILSPEG
                     YNYVTDAFGYYSITNNWNAAEAMQFIYADFTGNPQYKTLNEGYDYLPYLVRQEFVAAG
                     GEVRLNTPVRAVNRNSEGLMEVWLEGADTPQLARHVILAMPRRSLELLERTPLWQMDR
                     VVGASGTLQNYLRSVIIYPAFKMFLAFETPWWRNPPISMAAGRTVSDQPIRQTYYFPP
                     LPGGFQPGKAPMDGPCLVMASYDDLNAVPFWQSLEDPQDLKAVAKTQMMATLPGAASN
                     ADTGPLQAHIAETNAALADEPGFHYAPPEMVRYALEQLQLVHYNQPLPDPMILPGNLK
                     GYFLAAYKDWGHDPFGGGWNFWAPQTDVRDVMQRIRRPFTDANLYIVGDAYSGAQGWV
                     EGALTTAETMLREQFDLAEDPTWQPPGYYMGY"
     gene            complement(2477..4369)
                     /locus_tag="VFH60_03135"
     CDS             complement(2477..4369)
                     /locus_tag="VFH60_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015114129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET6312804.1"
                     /translation="MATEQPPQGQRWTRDYLYDILRDLGIHYLFGVPGTNEVPLIDGS
                     ELPENEVTYVPCLHENIAMGAAMGYARMTGKPGVLVLHVTPGAGHSLGNLFNAWKSRV
                     PLVILCGQQQNELVTQEPLLASNVVQIVSQFTKWSHELRAWEEMGMVLQRAFKEAMAP
                     PAGPVFISFPWEFTIRRIEADDHLPGVTRIPSRFTGDPEAVQQAADLLAKAKSPVIVA
                     GDGVGYSDAWKELQQLAELIGAPVYLEGFSSMANFPNFDYHWQGELPGDQMQTQSRFN
                     LHDVAFLCGFGAQAQVTVFKYSDGPLIPATVQTIYLHNNAWEIGKNEYGQAAILGDIK
                     ATLPGLNALIAKNRPPGADARNKTLQAYAQQRAQQWETYLQQSAGQEPIVAVVVANAL
                     RQLIEKHNLQKKFVYVHEAISDPTPFQFYLPFGEPYAEPTSYYCVEGGSLGWSMPATL
                     GIKLAEEGHQGIQPDLVVNAVGDGSSLFYPQVWWTAAQHKLPILYIIMNNREYRTLLQ
                     GVLGVVKYYGSDPDYGWNPVSTEPEYLKLGNPDFDFVKIAAAFSVPNGRRVRDPKEVM
                     PALEEAFEYVLGRKQPYVLELFTDPNPKSVVPSTVLEGADTDVTQSEVAAPPLDIFYH
                     KEANLL"
     gene            complement(5174..>9794)
                     /locus_tag="VFH60_03140"
     CDS             complement(5174..>9794)
                     /locus_tag="VFH60_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 66 protein"
                     /protein_id="HET6312805.1"
                     /translation="ASALPRPAVLTLEPTVAHAATTGTLITRVYTDKARYNPGNTVVI
                     TAELKNTTGASWTGTLALSIKKLETQVHTATSTSITLANNATTTRTFNWTAPATDFTG
                     YYAGITAGVVDFHGTGIDVSTSPYRFPRYGYISDFPSSRTSTQSTNMIKQMSEDYHIN
                     MFQFYDWMWRHEKMFKRTGGTVDSSWVDLFNRTISWPTIQNDISAVHAYNANAMAYAM
                     SYAAREGYQQMWGIDPAWGMYNDTAHASQLNVDFKNGAFLWLFNPANVNWQNYIINEY
                     KDAINTAAFDGIQIDQMGQRTGVYDYNGFSHYIPPTFPQFQQSVKAALVANNAARAGM
                     TFNIVDGTVDGWAANHVSTYGASDFDFSEIWYLSNSYNQLRNYVEQLKRNNGGKAVVL
                     AGYMNYGENLGPKSEAETATRVGVTTNTNHPGYTGTGFVDGFDAVGESITWSVSFPET
                     GDYSFDFRYANDIGATATRNVYVDGTLLGQVSFSDQANWDTWAHDAWIQKTSLTAGTH
                     TVKLAYDSGNSGAINVDHLTLGTFDDHSIRLADAMMFASGATHIELGDQNHMLAHEYY
                     PNKSKSMRNSLKSAMKDYYSFATAYENLLFDADVVPADQGAQWLSLTTGQTLSGNATS
                     GTVWEMVKRKPGYDIIHLINLIGNDDQWRNSGVQPTFQTNVGVKYYTGPNATVSGVYL
                     ASPDNDHGVTSSLTYTSGSDTQGNYVQFTIPSLKYWDMVYVKRTVTAPGGGQYEAESA
                     IKSAVTTNTPPAGYTGTGFVDGNSVSGDGVSFLVNVPANDSYTLRFRYANGGTGNATR
                     GLFVDGQGAGTVSFRNHNHWAVWGTAEKVVRLSAGVHQVVLWYGTGNTGAVNLDNLVV
                     LRETTPAAASATSLWMNNWSNMIGIHMASKLSPADTGTFGPRLAEIRFSSDWPTNQIV
                     DSTAFFRDQTGTLVKYTDAHQFDSEAWIETDGTLTAQYLNYGTSALPVKITKQYAMVP
                     NQNFIVVKYTLQNLTTSSRTINFLEQLHLNNKTKNNPTPGWQHGWWDVSRNALGTDMS
                     QTGQYYMEFGAFQAMDTRQVGNDSDSNTANTTSGAWYQFDANGTLKNNGDLWSQNLDL
                     GFQKTLTITAGSSASVAFYYTIGTNQAAAESAADTARAQTADYWLGQTATQYTNWLNA
                     GRRTSMTDAGLNTAFDRSLVINKQSQQPQYGSWPAATNPAYEYKVWVRDAAVTAMGMD
                     ATNHLAEAEKYWNWMASVQSTDGTWHTNYSAWHANQWISFVEPEHDAIGLFLMGVYRH
                     YNIVKATNPTAATTFLNGVWTQVTRSGDWIRNNIGANGLGAADASIWEEAIEYNTFTQ
                     VTYAEGLNAARYLATEKGDTTRATNYLSSAQTIKNATLRSFTASPHRGLWNDSTRSFN
                     RAVNTDGTARTLVDASSDLLWVFGMLPATDSRVRDHRIKVLSTLTHDGYGVARYEQDP
                     FYYSSPYSPGGMYEAAAAEPVWPQMSMYAAMLEHWQGNDAMSLARLQWYASRTGRGFV
                     TPGESIDWTTGLPLVSTMAEPVTGAWYQLAVLNYLNLFDPRLPGF"
BASE COUNT         1701 a         2884 c         3158 g         2051 t
ORIGIN      
        1 cgctgaagag ggcagccagc cacctggggg tgcggtcggc ggtgaagagg gcgctccact
       61 cgacgaagtg gctatcggca agcggtctgg tgtcaatagg tacggtgtag agcgcgaaca
      121 ccgcgtaagc cgacagcgcc agcgtaaccc acagcgcgat agctgcggtg cgccctctga
      181 acagcaggaa catcgtgccc agggccagga aaccgacgtt caagatggcc ccggtgaggt
      241 aatacgtgcg tgccaggaag gggttccagc cacccgaaac gtcggcgtat acctggcagg
      301 ccgcccctat cgcgaacagg agaaaggcca ccgcccaggc aagttcgtgc ggcctgcgct
      361 tgccgccgaa gtagcggttc agcagcacga tggcaaacgc cccggcgatt accgtggctg
      421 ataggggcaa catcgtcagg actgggaaaa cgggcatcaa cgctcctcct gtcagttctc
      481 cgggcgcgcc acggactagc ctctccgcag gcacatacag tgtacacaag gcgcgagtgg
      541 gaggcaagtc tgcgagcgtt aatattccat atacttagcc ttgccctact cccaggagca
      601 agcagggtaa ggggacagtc cccacaccct gcttgttgct ccatatggcg tcaccgcaac
      661 cgcccggcgg tcgcgtcgct cagctagtag cccatgtagt agccaggagg ctgccaggtc
      721 gggtcttccg ccaggtcgaa ctgctcgcgc agcatggtct ctgcggtggt gagtgcccct
      781 tctacccagc cctgggctcc cgaataggca tcgcccacga tgtacaggtt ggcatccgtg
      841 aacggcctgc ggattcgctg catcacgtcc ctgacatccg tctgtggtgc ccagaagttc
      901 caaccaccgc cgaacgggtc gtgcccccag tccttgtagg cggcgaggaa gtaacccttg
      961 agatttccgg gcagaatcat gggatcagga agcggctggt tgtagtggac gagctgcaac
     1021 tgctcgaggg cgtaacgcac catttccggc ggtgcgtagt ggaacccagg ctcatcggca
     1081 agcgcggcgt tcgtctcagc gatgtgggcc tgtaacgggc ctgtatccgc attggaagca
     1141 gccccgggca acgtcgccat catctgcgtc ttggcgacag ccttaaggtc ctgtgggtct
     1201 tccagactct gccagaacgg caccgcgttc aggtcgtcgt aagatgccat caccaggcag
     1261 gggccgtcca taggggcctt gcccggctgg aacccgcccg gtagcggcgg gaagtagtag
     1321 gtctgccgga taggttggtc ggagacggtg cggcccgccg ccatactgat aggcggattg
     1381 cgccaccagg gggtctcgaa cgccaggaac atcttgaacg ccgggtagat tatcaccgag
     1441 cgcaggtagt tctgcagtgt ccccgacgcg ccgactacac ggtccatctg ccacagcggg
     1501 gtcctctcca gcagttccag cgaacggcgt ggcatggcga gtatcacgtg ccgggcgagc
     1561 tgaggcgtat ccgcgccctc gagccacacc tccattagac cttccgagtt ccggttgacc
     1621 gcgcgcacgg gcgtattcag cctgacctca ccacccgctg ccacaaactc ctggcgcacc
     1681 aggtacggaa gatagtcgta gccctcgttg agcgtcttat actgggggtt gccggtaaag
     1741 tcggcataaa tgaactgcat cgcctcggct gcattccagt tgttggtgat gctgtaatag
     1801 ccgaaagcgt ccgtcacata gttgtagccc tcgggcgata gtatatccca gagcaggttc
     1861 cagaacccca catcccacat ccgccgcgac ccatagcgca agtaaggttt gacctcgtcc
     1921 cattcctgcc agctcatcgg gcgggtcggc cctcccagcc gttcggctat cacctccctg
     1981 ttggccgcca gcatacgtcc tatcacgtca ttgatgatcg tgggtggatc ggcgtagcgg
     2041 gcgcgcgcgt cgaggcggta tggcactttg tccgggttgg tcagatcggg agccttgagg
     2101 ttgttctcgc gcaggtacca gatgttgtta ccgttggggt cggtcacgta aaagtcaatc
     2161 ggcggttgta gtttaccctg tttgacaaag tggttgttga tgaggcccca cacaaggccc
     2221 tgctcttggt agaagcgcat gccgccgagc tcggcgttca ggcccggctg ttcggggaac
     2281 ggcctccagg ttaggagcct gccccccgtg cgcccactca actcgaacac agtgactttc
     2341 tttatcggcc gtccgccctt agccttgccc cggcccttgg actcggtctt gccggtgagc
     2401 agccgccacg ccgaatacag gcccgatggg ccgccaccga caacggctac gtcgaggatg
     2461 tcatcttgat tgaccatcat agcaggttcg cctccttgtg gtagaaaatg tcgagcggcg
     2521 gggccgctac ctcgctctgc gtcacatcag tgtcggctcc ttccaacacc gtgctcggca
     2581 ccaccgactt ggggttcggg tcggtaaata gctccagcac gtacggttgc ttcctgccca
     2641 ggacgtactc gaacgcctct tccagggcgg gcatgacctc tttagggtcg cgcacgcgcc
     2701 gcccgttggg cacgctaaag gccgctgcga tcttgacaaa atcgaagtca gggttcccaa
     2761 gcttcaagta ctccggctcg gtcgaaacgg gattccagcc gtagtctggg tccgagccgt
     2821 agtacttgac gacccccagc accccttgca gcagcgtccg gtactcccgg ttgttcatga
     2881 tgatgtacag tatggggagc ttgtgctggg ccgccgtcca ccacacctgc gggtagaaca
     2941 gcgaagagcc gtcgcccacc gcgttcacca caaggtcagg ttgtataccc tggtgccctt
     3001 cttccgccag cttgatgccc aacgtggcgg gcatggacca cccaagcgac cctccctcca
     3061 cgcagtaata gctggtcggc tcggcgtaag gctctccgaa cggtaagtag aactggaagg
     3121 gggtgggatc ggagatcgcc tcgtgcacgt agacgaactt cttctgcaag ttatgtttct
     3181 cgatgagctg cctgagcgcg ttggcgacta ccacggcgac tataggttcc tggcccgccg
     3241 actgttgcag gtaagtctcc cattgttgcg cccgctgctg ggcgtaggct tgcaaggtct
     3301 tgttgcgggc gtccgcgccg ggtggccggt tcttggcgat gagggcgttg aggcccggca
     3361 acgtggcctt tatgtcgccc aggattgccg cctgaccgta ctcgttcttg cctatctccc
     3421 aggcgttgtt gtgcaggtaa atcgtctgta ccgtcgcggg aatcaggggg ccgtcggagt
     3481 acttgaacac cgtcacctgc gcctgcgccc cgaagccaca gaggaacgcc acgtcgtgca
     3541 aattgaagcg gctctgtgtc tgcatttggt ccccgggcaa ctccccctgc cagtggtagt
     3601 cgaagttcgg gaagttagcc atgctgctga aaccttccag gtacacaggc gcgcctatca
     3661 gctcggcaag ctgctgcaac tctttccagg catcggagta gccgaccccg tcccctgcta
     3721 ctatcacagg actcttagcc ttcgccagca ggtcggcggc ctgttgcacc gcctctgggt
     3781 ctccggtaaa gcgcgagggg atgcgcgtga cgccgggcag gtggtcgtca gcttctatgc
     3841 gccggatggt gaactcccag gggaacgaaa tgaagacagg cccggcgggt ggtgccatag
     3901 cctctttgaa ggccctctgc agtaccatgc ccatttcttc ccaggcccga agctcgtggg
     3961 accactttgt aaactggctc actatctgca ccacgttgga ggcgagtagt ggctcctggg
     4021 ttacaagctc gttctgctgc tggccgcaga ggataaccag gggcaccctc gacttccagg
     4081 cgttgaacag attgccgagg ctgtggcccg cgccaggcgt tacgtggagt accagaaccc
     4141 ccggcttgcc cgtcatacga gcgtacccca tcgcggcccc catcgctatg ttctcgtgaa
     4201 ggcacgggac gtaagtgacc tcgttttccg gtagctcact cccgtctatc agtgggacct
     4261 cgttggtgcc gggcacaccg aacaaataat ggatgccgag gtctcgaagg atgtcgtaca
     4321 ggtaatcgcg agtccaccgc tggccttgtg gtggttgttc cgttgccatg tcttacctcc
     4381 tgtggagacg tccaggcccg cacagtcttc agtacaggcc aaactcaagc taccgcatcg
     4441 cgactcgaag tagctgcccg cgtccctggc gcggccgagc ctcggtgcga ctatgcgtcc
     4501 ttttctaagc atattccgaa agccactcac cacctccctt caacatgggc ttgccttttt
     4561 cgggcactgg aatgcgccgg acacaccggc ccgacggacc tcatcggtgc ggcggaatcc
     4621 acgatgatag cgtacgccca catgtatatg agcgagcgac atgcaccgcc tatgggcggg
     4681 tccaattggg tccgcaaaga ccccctgcaa caggcccggt tatctcgatg ggccgtgtag
     4741 gggagacatc aaagcataag ttacgactgt gtgcgacact agaaatgaag gttagttcac
     4801 agctacctcg ggacagccgt tagtgttaga gcctagcata tcggcctgcg tgttgtcaat
     4861 agacatttgc gctctgctgc tagggatata cggcgtgact ttgtagtaca tagtcgcggc
     4921 ttctgtaaca ctacgatgca actgcttgga gccgggcagg gcctgggacg tgcgaagtgt
     4981 ctgccggcaa ggaggtaaga ggcgtgagat gcgggtctat cggggtgaaa aagctccagg
     5041 tgccctcctg gagctacagg gagtgtgccc cggcgaggaa gattgtagaa aaccggggcc
     5101 gcaacacgaa gatcgcggcc ccaattttcg aagctctact gattgacatt gttaggctag
     5161 aaggcccgtg ctcctagaag cccggcagcc tcgggtcgaa caggttgagg tagttcagca
     5221 cggccaactg gtaccacgct cccgtgacag gttccgccat tgtgctgaca aggggcaggc
     5281 ccgttgtcca gtcgatggac tcgcccggag tgacgaaccc gcgcccggtg cggctggcgt
     5341 accactgcaa gcgggccagg gacatggcgt cgttaccctg ccagtgctcc agcatggccg
     5401 cgtacatgct catctgaggc cataccggct cggcagccgc agcttcgtac atgccgcctg
     5461 ggctgtacgg gctggagtag tagaaggggt cctgctcgta acgggccacg ccgtagccgt
     5521 catgggtcag ggtgctgagc accttgatcc tgtggtcgcg cacccgcgag tcggtggcag
     5581 gcagcatacc gaacacccac agcaggtccg acgaggcgtc caccagggtg cgcgccgtgc
     5641 cgtcggtgtt caccgcccga ttgaacgagc gggtcgagtc gttccacagg ccacggtggg
     5701 gcgaggcggt aaaggagcgc agggtggcgt tctttatcgt ctgcgcactc gacaggtagt
     5761 tggttgccct cgtcgtgtcg cccttctctg ttgcaaggta gcgggcggcg ttcaggcctt
     5821 ccgcgtaggt cacctgggtg aaggtattgt actcgattgc ctcctcccag atcgaggcgt
     5881 cggcggcccc caagccgtta gcgccgatgt tgttcctgat ccagtcgccg gagcgggtca
     5941 cctgcgtcca gacgccgttc aggaaggtcg tggccgcagt ggggtttgtg gcctttacga
     6001 tgttgtagtg ccggtagacg cccatcagga agaggccgat ggcatcgtgc tcaggctcca
     6061 cgaaggagat ccactggttg gcgtgccagg cgctatagtt ggtgtgccag gtgccgtcgg
     6121 tgctctgcac cgacgccatc cagttccagt acttctcggc ctcggccagg tggttggtgg
     6181 cgtccatgcc catagcggtc accgccgcgt cgcgcaccca caccttgtac tcgtaggccg
     6241 ggttggtagc cgcgggccac gagccgtact ggggctgttg cgactgcttg ttgatcacca
     6301 ggctgcggtc gaaagcagtg ttcaggcccg catcggtcat gctggtgcgc ctgcccgcgt
     6361 tgagccagtt ggtgtactga gtggcggtct ggccgagcca gtagtcggcc gtctgcgcgc
     6421 gggcggtatc ggctgccgac tcggcggcgg cctggttggt gccgatcgtg tagtagaagg
     6481 cgacagaggc gctgctgccc gcggtgatgg ttagcgtctt ctggaagccg aggtcgaggt
     6541 tctgcgacca cagatcgccg ttgttcttca gcgtgccgtt cgcgtcgaac tggtaccacg
     6601 cgcccgaagt agtattggcg gtgttggagt cggaatcgtt gcccacctgg cgggtgtcca
     6661 tcgcctggaa cgcgccgaac tccatgtagt actggcccgt ctggctcatg tcggtgccca
     6721 gcgcgttgcg gctaacgtcc caccagccat gttgccagcc gggtgtgggg ttgttcttag
     6781 tcttgttgtt caggtgtagc tgttccagga agttgatggt gcgcgagcta gtcgtcaggt
     6841 tctggagtgt gtacttgacc acgatgaagt tctggttggg caccatcgcg tactgcttgg
     6901 taatctttac gggcagcgcg ctggtgccgt agttgaggta ctgtgcagtc agcgtgccgt
     6961 cggtctctat ccaggcttcg gagtcgaact ggtgggcgtc ggtgtacttg acaagcgtgc
     7021 cggtctggtc gcggaagaaa gcggtggagt cgactatctg gttggtaggc cagtcgctgc
     7081 tgaagcgaat ctccgctaag cgaggcccga aggtgccggt atccgcaggg cttagtttgc
     7141 tagccatgtg gatgccgatc atgttgctcc agttgttcat ccacaggctc gtggccgacg
     7201 ccgccgccgg cgtggtctcg cgcagcacca ccaggttatc caggttgaca gccccggtgt
     7261 tgccggtgcc gtaccatagc accacctggt gcacaccggc cgagaggcgc accacctttt
     7321 ccgccgtgcc ccagacggcc cagtggttgt ggttacggaa gctcaccgta cccgcgccct
     7381 ggccgtccac gaacagcccg cgggtggcgt tgcccgtgcc gccgtttgca tagcggaagc
     7441 gcagggtgta gctgtcgttg gccgggacgt tcaccaggaa cgatacgccg tcgccgctga
     7501 cactgttgcc gtccacgaag cccgtgccgg tgtagcccgc ggggggggtg ttggtggtga
     7561 ccgccgactt gatggccgat tctgcctcgt actggccgcc acccggcgcg gtaacggtgc
     7621 gcttcacgta caccatgtcc cagtacttga ggctcggtat tgtgaactgc acgtagttgc
     7681 cttgcgtgtc gctgccgctc gtataggtga gggaagaggt cacgccgtga tcgttgtccg
     7741 ggcttgccaa gtacacgccg ctcacggtag cgttggggcc ggtgtagtac ttgacgccca
     7801 cgttggtctg gaaggtgggc tgcacgccgg agttgcgcca ctggtcgtcg ttacctataa
     7861 ggttgatcag gtggatgatg tcgtagccgg gcttgcgctt caccatctcc cacaccgtgc
     7921 cggaggtggc gttgccgctc agcgtctggc cggtggtcag cgagagccac tgcgcgccct
     7981 ggtcggcggg taccacgtcg gcatcgaaca ggaggttctc ataagcggtg gcgaagctgt
     8041 aatagtcttt catcgcgctc ttgagggagt tgcgcatgct cttcgacttg ttgggatagt
     8101 actcgtgagc aagcatgtgg ttctggtcgc caagctcgat gtgggtagcg ccactggcga
     8161 acatcatagc gtccgcgagg cgtatggaat ggtcgtcgaa ggtccccaac gtcaggtggt
     8221 ccacattgat ggcaccggag ttgcccgagt cgtaggcgag tttcacggtg tgggtaccgg
     8281 cggtcaggct ggtcttctgt atccaggcgt cgtgcgccca ggtgtcccag ttagcctggt
     8341 ccgagaatga gacctggcct agaagcgtgc cgtccacgta cacgttgcgg gtagccgtgg
     8401 caccgatgtc attggcgtag cggaagtcga acgaatagtc gcccgtctcc gggaagctga
     8461 cgctccaggt gatggactcg cctaccgcgt cgaagccgtc aacgaagccg gtgcccgtgt
     8521 agcccgggtg gttggtgttg gtggtcacgc ccacgcgagt ggcggtctcg gcctcgctct
     8581 tggggccgag gttctcgccg tagttcatat acccggccag cacgacggcc ttgcccccgt
     8641 tgttgcgctt cagttgctcg acgtagttgc gtagctggtt gtagctgttg gagaggtacc
     8701 atatctcgct gaagtcgaag tcgctcgccc cgtaggtcga gacatggttg gcggcccagc
     8761 catccaccgt gccgtccacg atgttgaacg tcatccccgc cctggcggcg ttgttcgcta
     8821 cgagggccgc cttgaccgac tgctggaact gcgggaaagt aggcgggatg taatgtgaga
     8881 agccgttata gtcgtagacg ccggtgcgct ggcccatctg gtcgatttgt atcccgtcga
     8941 aggcggcggt gtttatcgcg tccttgtact cgttgatgat gtagttctgc cagttgacgt
     9001 tggcggggtt gaacagccag aggaacgccc cgttcttgaa atcgacgttt agctggctgg
     9061 cgtgcgcggt gtcgttatac atgccccagg caggatcgat accccacatc tgctggtacc
     9121 cctcgcgggc cgcgtagctc atcgcgtagg ccatggcatt ggcgttgtag gcgtgcacgg
     9181 ccgagatgtc attttgtatc gtaggccagg agatagtgcg gttgaacagg tccacccagc
     9241 tcgaatcgac ggtgccgccg gtgcgcttga acatcttctc gtgccgccac atccagtcgt
     9301 agaattggaa catgttgatg tggtagtcct ccgacatctg ctttatcatg ttggtgcttt
     9361 gcgtggaggt gcgcgaggag ggaaagtcgg atatatagcc gtagcgcggg aagcggtagg
     9421 gggaggtgct tacgtcgatg ccggtgccgt ggaagtctac cacaccggcg gtgatgcctg
     9481 cgtaataccc ggtgaagtcg gtggcgggcg cggtccagtt gaaggtgcgc gtggtcgtag
     9541 cgttgttggc cagggtaatg cttgtgctgg tggcggtgtg gacctgcgtc tccagcttct
     9601 tgatgctgag ggcgagcgtg cccgtccacg aagcgccggt ggtattcttt agctcggccg
     9661 taatgaccac cgtgttgccg gggttgtacc gggccttgtc ggtgtacacg cgagtgatga
     9721 gcgtgccggt ggtagcggcg tgggctacgg taggctccag cgtcaacacc gccgggcgtg
     9781 ggagggccga agcc
//
LOCUS       DASRWZ010000205         3743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_221958, whole genome shotgun sequence.
ACCESSION   DASRWZ010000205 DASRWZ010000000
VERSION     DASRWZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221958"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1673
                     /gene="araA"
                     /locus_tag="VFH60_03145"
     CDS             156..1673
                     /gene="araA"
                     /locus_tag="VFH60_03145"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HET6312806.1"
                     /translation="MIDLSQYEVWFVTGSQDLYGPETLEKVAEHSRQIAAGLSASTAI
                     PVKVVFKPVLTGPDEIRQLCLEANSTRNCIGLVAWMHTFSPAKMWIAGLASLQKPFVH
                     LHTQYNREIPWSEIDMDFMNLNQSAHGDREFGYIGSRLRLNRKIVVGHWQDEEVRSDL
                     GAWARAAAAWHDAQGARFARFGDNMREVAVTEGDKVEAQLRLGYSVNGYGLGELVRYV
                     NEVPGAEVERLIEEYRERYQVSPDLGKDGGSSNYRSLWEGARLEAGIRAFLEEGNFKG
                     FTTTFEDLHGLHQLPGLAAQRLMAEGYGYGAEGDWKTAALVRAMKVMDAGLEGGTSFM
                     EDYTYHFGSSGMKILGSHMLEVCETIASPAEKPRLEVHPLSIGGKADPARLVFDGRMG
                     PAVAASIVDVGNRFRMVVNAVDAVPVEAPLPKLPVARVLWTPQPDLKTAATAWIMAGG
                     AHHTGFSFALTPQHLEDFADMAGMEFLLIDRDTRLRDFKKEIHWNDVYYSLARGF"
     gene            2120..3595
                     /locus_tag="VFH60_03150"
     CDS             2120..3595
                     /locus_tag="VFH60_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6312807.1"
                     /translation="MVLQRRTHKFLRLVTLFLLMATFLAGCGGETAPTQVPAAPTGTT
                     GSQEVATTAPVDEATATMAAAPATDTATTTGAATTEPTAAPNPNITPNPALSAKLTVW
                     GWQATVDALKAAEADFKQAYPNIEVEYVPRPPADTYQQLQLAASAGTGLPDISVIEDS
                     HLAQFVGLGVLADVSEEVQPYIPQFNEYRWQQAMLDGKYYAMPWDSGPVAVFYRRDVF
                     EKAGVDPESIKTWDDYYAAAKTIKEKAGVPMWQQSKAKNDGRLFEMLMWQEGLGYVDK
                     DGNVILDKEPRIAEILDYMGKFWQEDLAADSEPWTDPWYKEMADGTVATVVEAAWMGG
                     FFKSFIAPEAKGKWGVFRLPAWQAGGSQSSNDGGSSLAIFETSQQKEAAWAYVEFHMT
                     RPDEMIKIYEQSDFYPGLQTTYNDPAFQEADPYYAGQKVRAFFSEVAREIPTAGVYTA
                     EYQEMNGLLTPEIQKFALGQQTSQEALTNAANAIRDKTGRK"
BASE COUNT          776 a         1128 c         1218 g          621 t
ORIGIN      
        1 cttcaactgg gcacccccga ggagataccg cccgaggacg tggcaaagct ccaccaccgt
       61 tacacgaacg tctacggcca atagacacac gggcacaaag aggcaacgct ttatacatag
      121 ccttataccg aaacgctgac gaggagaaac gttagatgat cgatcttagc cagtatgagg
      181 tgtggttcgt taccggcagc caggacctgt acggtcctga gacgcttgag aaagtagccg
      241 agcactcccg gcagatcgcg gccgggctgt cggcgtcgac tgccatcccc gtcaaggtgg
      301 tgttcaagcc ggtgctgacc ggcccggacg agatacggca gctatgcctc gaggccaact
      361 ctacccgcaa ctgcatcggc ttggtcgcgt ggatgcacac attctccccg gccaagatgt
      421 ggatagcggg gctggcttcg ctccagaagc cgttcgtaca cctgcacacg cagtacaacc
      481 gcgagatacc ctggtccgag atcgacatgg acttcatgaa cctcaaccag tcggcgcacg
      541 gggaccgcga gttcggttac atcggcagcc gcttgcgtct caaccgcaag atcgtggttg
      601 ggcactggca ggacgaggag gtgcggtcgg acctgggcgc gtgggcgcgg gcagccgccg
      661 cctggcacga cgcgcagggg gccaggttcg cgcgcttcgg agacaacatg cgcgaggtgg
      721 ccgtaacgga gggcgacaaa gtggaggccc agttgcggct ggggtactcc gtcaacggct
      781 acgggttggg tgaactggtc aggtacgtca atgaggtgcc gggcgcggaa gtggagcggc
      841 tgatagagga gtaccgggag cggtaccagg tctcgcctga cctgggcaag gacggcggta
      901 gcagcaatta ccgttcgttg tgggagggcg cgcggctgga ggcaggcata cgggctttcc
      961 tcgaagaagg caacttcaaa ggcttcacta ccaccttcga ggacttgcat ggcctccacc
     1021 agttgccggg gctggcggcg cagcgcctca tggcagaggg gtacggctat ggtgccgagg
     1081 gcgactggaa gacggccgcg ctcgtacgcg ccatgaaggt catggacgcg gggctggagg
     1141 gcggcacttc ctttatggag gactacacct accacttcgg ctctagcggc atgaagatac
     1201 tcggctcgca tatgctggag gtctgcgaga cgatagcctc ccccgccgag aagcccaggc
     1261 tggaggtcca cccactctcc atagggggca aggccgaccc ggcgcggctg gtgttcgacg
     1321 gcaggatggg gcctgccgtg gcggcatcta tcgtggacgt aggcaatcgg ttccggatgg
     1381 tggtcaacgc ggtggatgcc gtgcctgtgg aggctcccct gcccaaattg cccgtagcca
     1441 gggtgctctg gactccgcag cccgacctga agacggcggc caccgcctgg atcatggcgg
     1501 gcggggcgca ccacaccggc ttcagctttg ccctcacgcc ccagcacctg gaagacttcg
     1561 cggatatggc gggcatggag ttcttgctga ttgatcgcga tacccggctg cgtgacttca
     1621 agaaagagat acattggaac gacgtgtact actcacttgc caggggcttc taacgtgaga
     1681 agtcacggct gtgtacaagt agaagagcag cactgtgttg gccccctgtc attctgagcg
     1741 tagcgaagaa tctaaagaag gtgggaaagg atgccgccgg acattacgtc ctatgatgct
     1801 gacgttcaac gtagaggtat gagtggcatc ttagattctt cgctacgctc agaatgacag
     1861 gggccacctg taccctgaaa attcgacttg gatgcggacg tacctggcaa attatgtcga
     1921 tggagtgaag caatgagcac acgcaacacg cccttgacgg gtctgcgcgc taagttccac
     1981 ggacggtccc cgccggggtc cctctacatg gtgaccgcat gagcctgcct ctaacagggg
     2041 ttgccggccg cccgcaccga gcggcagcac cgcgcggcta attggcttct atcgtccaaa
     2101 gaaggaaagg agactaaacg tggttttgca aagacgcact cacaagttcc tacgcctggt
     2161 gacgcttttc ctgctcatgg caacattcct cgcgggttgc ggcggagaga ctgcacccac
     2221 ccaggtgccc gctgccccca ccggtactac cgggagccag gaggttgcca ctacggcacc
     2281 tgtcgatgag gccaccgcaa cgatggctgc tgctccggcg actgacacgg cgaccacaac
     2341 gggcgcggcc accaccgagc ctaccgccgc accgaacccc aatatcacgc cgaatcccgc
     2401 cctctcggcc aagctgaccg tctggggttg gcaggccacg gtggatgcgt tgaaggctgc
     2461 ggaggccgat ttcaagcagg catatcccaa catcgaggtg gagtacgtgc cccgcccgcc
     2521 cgcggatacc taccagcagc tccagcttgc agcttccgcg ggcaccggcc tacccgacat
     2581 ttcggtcatc gaggactccc acctggcgca gttcgtgggc ctcggggtgc tggccgacgt
     2641 tagcgaggag gtccagccct acatcccgca attcaacgag taccgctggc agcaggctat
     2701 gctggacggc aaatattacg cgatgccgtg ggacagcggc ccagtagccg tgttctaccg
     2761 gcgcgacgtg ttcgagaagg cgggcgtcga tcctgagtct atcaagacgt gggacgacta
     2821 ctacgcggcc gccaagacga tcaaggagaa ggccggggtg ccgatgtggc agcagtccaa
     2881 ggccaagaac gacggccgcc tgttcgagat gctcatgtgg caggaagggc ttggctacgt
     2941 ggacaaggac ggcaacgtca tcctcgacaa ggagccgcgt atcgcggaga tactggacta
     3001 catgggcaag ttctggcagg aagacctggc cgccgacagc gagccgtgga ccgacccctg
     3061 gtacaaggag atggccgacg gcacggtggc gacggtagtg gaggcagcct ggatgggcgg
     3121 tttcttcaag agcttcatcg cgccggaagc caagggcaag tggggcgtct tcaggctccc
     3181 cgcctggcag gcaggtggct ctcagtcgag caacgacggt gggtcctcgc tggcgatctt
     3241 cgaaacgagc cagcagaagg aagccgcctg ggcctacgtg gagttccaca tgacgcgccc
     3301 cgatgagatg atcaagatct acgaacagtc agacttctac cccggcctgc aaaccaccta
     3361 caacgacccg gccttccagg aggccgaccc ctactacgcc ggccagaagg tgcgcgcctt
     3421 cttcagcgag gtagccaggg agataccgac ggcaggcgta tacaccgccg aataccagga
     3481 gatgaacggg ctactgacgc cggaaatcca gaagtttgcc ctgggccagc agacctcgca
     3541 ggaggcgctg acgaacgcag ccaacgcgat ccgggacaag acgggtcgca agtagaagga
     3601 gcatccgatg cgcaacactt cgtcgcagcc ggatctctca aagggcaact cgccggccgt
     3661 tccagccaag tgggcggccg gcggggccag gccccagcaa gcgcgcggtc tcatgtcgcg
     3721 gctgtggagc gcccgctggc cgc
//
LOCUS       DASRWZ010000206         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_839502, whole genome shotgun sequence.
ACCESSION   DASRWZ010000206 DASRWZ010000000
VERSION     DASRWZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839502"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="VFH60_03155"
     CDS             <1..1409
                     /locus_tag="VFH60_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HET6312808.1"
                     /translation="VDTPGTNAIIRRHEELTRTFVPRSDMVLFLTSADRPFTESERGF
                     METIREWGKKIVIVLNKIDLMQTPEDLDRVLHFIQENSLALLGQQPDIYPVSARLAQR
                     AREMAAGIERDAILGASRMEQLESYIFGTLDEAGRIRLKLSTPLGIAGRIAERYHQLT
                     RSRLAVLAEDFRTVENIEGQIGLYRQDMQREFEGRISQIENIIYEMRERGDSFFEETL
                     RMGRIRDLLKSDKIKREFEERVVSDSARRIDDAVSGLINWMVDQDLRLWQAVMQYVNR
                     RREAGVASTPGFDEHVIGNIGGQFESNRESLLRSVVLEARRVVETYDRRQQADAIAQD
                     MRAAVGQMVAAGGVAAVGVLVSIAFAAAFIDITGITAALVSGAAGLFVLPYRKRKAQQ
                     EFRERTQELRDKLVSAMSTQFNTELEHSTNRIREALAPYTRFVRLQQERMTEADANLS
                     RIEESLDSLRAQVETIGR"
     gene            1558..1905
                     /locus_tag="VFH60_03160"
     CDS             1558..1905
                     /locus_tag="VFH60_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312809.1"
                     /translation="MMADNPQNAQGDPEEQDVAKLGGPTAQAMGTEYQMQSEAGTKAA
                     QAAGEVSGEAAGAEHMEHAGRDPELYYSKQTAQGTADGGEMVDKPHDVIGEIGRHRQA
                     QPEAREEHGEKGT"
     gene            1975..2466
                     /locus_tag="VFH60_03165"
     CDS             1975..2466
                     /locus_tag="VFH60_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008949432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6312810.1"
                     /translation="MVRRATPEDGPTIVRLITALADFEKLPPPDEAAQARLLGDAFGP
                     RPRFEIFLAEHMGEVAGYAFGFETYSTFLALPTLYLEDLFVLPEHRGRKVGYTLFLHC
                     AREATRRGCGRFEWAVLDWNQHAISFYERLGARHLDDWRIYRLDREGLRALVHEEPDA
                     PQV"
BASE COUNT          534 a          809 c          881 g          434 t
ORIGIN      
        1 tagttgacac cccaggcacc aacgccatca tcaggcggca cgaagaattg acgcgcacct
       61 tcgtgccccg ctccgacatg gtgctcttcc tcacgtccgc cgaccgcccc ttcacggagt
      121 ccgagcgcgg tttcatggag acgatacgcg agtggggcaa gaagatcgtg atcgtgctca
      181 acaagatcga cctgatgcag acgccggaag acctcgaccg cgtgctccac ttcatccagg
      241 aaaacagcct ggccctcctg gggcagcaac cggacatcta ccccgtgtcg gcgcgactgg
      301 cgcagagggc aagggagatg gctgcgggca tcgagcgcga tgctatactg ggtgccagcc
      361 gcatggagca acttgagagt tatatcttcg gcaccctgga cgaagcgggc cgcatccgcc
      421 tcaagctgtc cacgccgctg ggcatagccg ggcgcatcgc ggagcggtac caccagttga
      481 cgcgcagccg gctggccgtg ctggccgagg acttcaggac ggtcgaaaac atcgagggtc
      541 agattgggct gtaccggcag gacatgcagc gcgagttcga gggccgcatc tcgcagattg
      601 agaacatcat ctacgagatg cgcgagcgtg gtgacagctt cttcgaagag accctccgca
      661 tgggccgcat ccgcgacttg ctcaagtccg acaagatcaa gcgcgagttc gaggaacggg
      721 tggtctccga cagcgcccgg cgaatagacg acgcggtgag cggcctcatc aactggatgg
      781 tagaccagga cttgcggctc tggcaggcgg tgatgcagta cgtcaaccgc aggcgagagg
      841 caggggtcgc ctccacgccc ggctttgacg agcacgtgat agggaacatc ggcgggcagt
      901 tcgagtcgaa ccgggagtcg ctgttgcgct cggtggtgct tgaggcgcgg cgcgtcgtgg
      961 aaacgtacga ccgcaggcag caggccgacg ccattgccca ggacatgcgg gcggcggtgg
     1021 ggcaaatggt ggcggcaggg ggtgtggcgg cggtgggggt gctggtgtca atcgcgtttg
     1081 cggcagcctt catcgacatc acgggcataa cggccgcgct cgtgtcgggt gcggcggggc
     1141 tgttcgtact gccctaccgc aagcgaaagg cacagcagga gttccgcgag cggacgcagg
     1201 agttacgcga caagctggta tccgccatga gcacgcagtt caacactgag cttgagcact
     1261 ccaccaaccg catccgcgag gctcttgccc cctacacccg tttcgttcgg cttcagcagg
     1321 agcgcatgac cgaggccgac gccaacctct ctcgcatcga ggagagcttg gactcgctac
     1381 gggcgcaggt ggagacaata ggcagataga tcggcactca ggagggccat gcagcctccc
     1441 atcgagaagg cggcccggca acacggtgca gtggtacttt cctgaggggt cctgtggttt
     1501 cccctgattc ccactttttc tcgtagttgt tcaacttata gtcgtagaag gagatgcatg
     1561 atggccgaca acccgcaaaa cgcacaaggc gacccggagg agcaggacgt cgcaaagctg
     1621 ggaggcccca ccgcgcaggc aatgggcacc gagtaccaga tgcagtcgga ggcgggcacg
     1681 aaagcggccc aggcggcggg cgaggtctcc ggcgaagcgg cgggggccga gcacatggag
     1741 cacgccggcc gcgatcccga gctttactac agcaagcaga ctgcgcaggg cacggcggac
     1801 ggcggcgaaa tggtggacaa gccccacgac gtgataggcg agatcggcag gcacagacag
     1861 gcccagccag aggccaggga agagcacggc gagaagggca cctgatagtc gtgccggtgg
     1921 gagcaagcaa tgactgacgt acagggtgca atctcatcca gtgggaccgc tgtgatggtc
     1981 cgccgagcaa cgccggagga cgggcctaca atcgtgcgcc tcatcacggc gctggccgac
     2041 ttcgagaagc tgccgccccc ggacgaggcc gcgcaggccc gcctcctggg tgacgcgttt
     2101 ggcccgcgcc cgcgcttcga gatttttctc gcggagcaca tgggagaggt agccgggtac
     2161 gccttcggat ttgaaaccta ctcgacgttc cttgccctac ccaccctcta ccttgaggac
     2221 ctgttcgtgc taccggagca ccgtggcaga aaggttggct acaccctctt cctccactgc
     2281 gccagggaag caacacgccg gggctgtggc cgcttcgagt gggccgtgct cgactggaac
     2341 cagcacgcca tcagcttcta cgagcggctg ggtgcgcgtc acctggacga ctggcgtatc
     2401 taccggctgg acagggaggg ccttcgggcg ttggtccatg aagagccaga cgcgccccaa
     2461 gtttgaggtg gaatccgcag gtcgcttggc atgaatggtc cgtgagtaga atgtgctgga
     2521 atggcgggac cactccccct cttgtcatcc tgagcgtagc gaaggatcag ccgcccattc
     2581 ttgacagcct cttctacggc accatagccc cacccttgtc atttcgaacg cagtgagaaa
     2641 tctcgtcctc tacaccga
//
LOCUS       DASRWZ010000207         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_427893, whole genome shotgun sequence.
ACCESSION   DASRWZ010000207 DASRWZ010000000
VERSION     DASRWZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427893"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2048)
                     /locus_tag="VFH60_03170"
     CDS             complement(<1..2048)
                     /locus_tag="VFH60_03170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6312811.1"
                     /translation="MLERLKLPRRLTSGLLTVDFLVLSALFLVAFARRWSGISGSLPY
                     VAHPDEPKVIDSAIHIVKSGDLNPNLYIWPSIYIYIQALLLKVHVVWGTLRGYYEGAA
                     SLPDVTHIFTLDSGVYIWARTLAAFVGAITIPVLYRVGKEMFNDSRRVGLAAALMLAV
                     SPLHVQYSHFALMDAPLGLMGIFVLWASYRLSRASASGKPLLRDPLFWQAALAGLLVG
                     VATGTKYNGLYLGVVPFIALVMAWRRNRYTLSRGRLLGAALGMGAFAALGFLLCEPYL
                     LLDWPAFYNGFTFQVQAYLPAENLDQVRASIERHVTDLAASDMHFFMPTALGAFVVLA
                     NAPVRNRFWLLVPFPVLYLLAMSRFSLTYVRNLIVVLPFLALLSGYVIDLVAMQLTTL
                     ARGYAARIEDRRVWNTVRWGIVGVALLFVTMEPLRISSNYSAAMADPDSRNLAWNWMQ
                     GKMREGYRFAAELHPWQVQDWPDVLAFDVENPGQTEELTARLPGWYGLYGYDYVVLNS
                     NLKDWQRDSANLPKYLTLPVVQKYLGDQEGGSKGPTVTVLDVRGPRDRPDPAPAAHDL
                     EAQVEDFARLVGFDLAPIANADILLEPGTVPLSYAFAPGQAIGLNMYYRALRDGSPAD
                     PPWQVWVHLVDPATGATVAQVDVPPLAGMLKQYPIINLERHPVSDWHHGELVAGIYNF
                     P"
BASE COUNT          387 a          618 c          774 g          347 t
ORIGIN      
        1 gggaagttgt agataccggc tactagctcg ccatggtgcc agtcgctgac ggggtgacgt
       61 tcgaggttga tgatggggta ctgcttgagc atgcccgcga ggggcggtac gtccacctgg
      121 gcgacggtgg cgcctgtggc ggggtccacg aggtgtaccc acacctgcca gggcgggtcg
      181 gcggggctgc cgtcgcgcaa ggcccggtag tacatattga ggccgattgc ctggcccggc
      241 gcgaaagcgt aactaagggg caccgtaccc ggctctaaca ggatatcggc gtttgcgatt
      301 ggggccaggt cgaagccgac cagcctggcg aagtcctcta cctgggcttc caggtcgtgg
      361 gccgccggtg ccgggtcggg ccggtctcgc ggcccgcgca cgtcgagcac ggttacggtt
      421 ggccccttgc tcccgccttc ctggtcgccc aggtatttct gtacgacggg caacgtcagg
      481 tacttgggca gattggcgct atcgcgctgc cagtccttga ggttggagtt gagcaccacg
      541 tagtcgtagc cgtacaggcc gtaccagccg gggaggcggg cggtaagctc ctccgtctgg
      601 ccggggttct ccacgtcgaa ggcgagcacg tcgggccagt cctgcacctg ccaggggtgc
      661 agctcggcgg cgaagcggta gccctcgcgc atcttcccct gcatccagtt ccaggccagg
      721 ttgcggctgt cggggtcggc catggccgcg ctgtagttgc tggagatacg tagcggctcc
      781 atcgtcacga acagcagggc tactcccacg atgccccagc gcacggtgtt ccacacacgg
      841 cggtcctcga tgcgggccgc gtagccccgt gcaagtgtgg taagctgcat cgccacaagg
      901 tctatgacgt agcctgagag gagagccagg aagggcaaga cgacgatcag gttgcgcacg
      961 taggtgagcg agaagcggct catcgccagc aggtagagca cggggaaagg caccagcagc
     1021 cagaagcggt tgcgcacggg ggcgttcgcc agcaccacga aagcccccag cgcggtaggc
     1081 atgaagaagt gcatgtcgct ggcggccagg tcggtgacgt gtctctctat ggaggcgcgc
     1141 acctggtcca ggttctcggc ggggaggtag gcttgcacct ggaaggtgaa gccgttgtag
     1201 aaggcgggcc agtcgagcag caggtacggc tcgcacaaga ggaagccgag cgcggcgaac
     1261 gcccccatgc ccagcgccgc ccccagcagt cgcccccggc tcagggtgta cctgttgcgc
     1321 ctccacgcca tgacgagcgc gatgaagggc accacgccga ggtagaggcc gttgtacttg
     1381 gtgccggtag cgacgcccac cagcagcccg gccaacgccg cctgccagaa gagggggtcg
     1441 cgcagtaggg gcttgccgga ggcagaggcg cggctcaggc ggtaggaggc ccagagcacg
     1501 aatatgccca tcaggccgag gggcgcgtcc atgagggcga agtgggagta ctgcacgtgg
     1561 aggggcgaca cggccagcat gagggccgcc gccagcccca cccgcctgct gtcgttgaac
     1621 atctccttgc cgacgcggta gagcaccggg atggttatgg cacccacgaa ggcggccagc
     1681 gtgcgcgccc atatgtacac gccggagtcg agcgtgaaga tgtgcgtcac gtcgggcagg
     1741 gaggcggcac cttcatagta gccccttaac gtgccccaga cgacgtgcac cttgagcagc
     1801 aacgcctgga tgtatatgta gatcgagggc cagatataga gattagggtt gaggtcgccc
     1861 gatttgacga tgtgtatcgc ggagtcgatc accttaggct cgtcggggtg ggccacgtag
     1921 ggcagagagc cggagatgcc ggaccagcgt cgcgcgaacg cgaccaggaa gagcgccgac
     1981 agtaccagga agtctacggt gaggaggccg gaggtgaggc ggcgcggtag tttcagcctt
     2041 tcgagcaaag gaaattatat catgttggcg gttgccggcg gcgaaagatg tcgtgggttg
     2101 ttcggggtcg tgtcagggta gggtgt
//
LOCUS       DASRWZ010000208         9844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1181934, whole genome shotgun sequence.
ACCESSION   DASRWZ010000208 DASRWZ010000000
VERSION     DASRWZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181934"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1029
                     /gene="lipA"
                     /locus_tag="VFH60_03175"
     CDS             64..1029
                     /gene="lipA"
                     /locus_tag="VFH60_03175"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET6312812.1"
                     /translation="MADTMNNVPGSPLELKPYSRPGGPARPISAHPAPIERKPEWLTV
                     KWQQGENYRDLKGLMRELGLHTVCEEAHCPNISECWEHRTATFLILGDTCTRSCGFCA
                     IKTGRPSKLDRQEPERVARAIEALQLKHAVITSVNRDELDDGGAEMFAEVIRLTREYV
                     PGCGLEVLIPDFKGVWWALQRVMDAQPDILNHNVETVPRLYYIVRPQAKYERSLELLQ
                     KARQMDAQGRSFTKSGIMIGLGEEWDEIVQTIRDLRDHDVDILTVGQYLRPSFRHLPV
                     LKYYTPAEFKELKEIALGLGFKHVESGPLVRSSYHAHEQVQAAGA"
     gene            1047..1751
                     /gene="lipB"
                     /locus_tag="VFH60_03180"
     CDS             1047..1751
                     /gene="lipB"
                     /locus_tag="VFH60_03180"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET6312813.1"
                     /translation="MADTIQRAESRTRPLSILDLGLIPYSECWELQRRLASERAEGAI
                     GDTLLLLEHPHTYTCGRRGGRDHILISEQELRERGISVLDVDRGGDVTYHGPGQLVAY
                     PIVRLAEEGQVADYHAYLRALEQVLITTLADFAIRADRIEGYSGVWVRSADSDEKIAA
                     IGVKVDGRGVSSHGIALNVNTDLQYFSHIVPCGIVEKGVTSMQRLLGTAPEMADVKSV
                     FSRAFCGVFGFSLALL"
     gene            1877..2146
                     /locus_tag="VFH60_03185"
     CDS             1877..2146
                     /locus_tag="VFH60_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET6312814.1"
                     /translation="MHATVRGRVQNVGFRMFARDAAQRLGVAGYVRNEHDGSVTVLAV
                     GAREALESLLVELRRGPAHARMERVEADWLPGNPQGFTGRFEVRP"
     gene            2146..3447
                     /locus_tag="VFH60_03190"
     CDS             2146..3447
                     /locus_tag="VFH60_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312815.1"
                     /translation="MSASVRADVVGTSDRDRARYVWLVLALSTLAFFGLCALVGNSIF
                     GFLTSVTERSAARLELRKGAQLTLQRFGSSASEVVADEAALEEGDRTTTGPGSEGYID
                     LFGGDITVYTYFSTTVTIDRLRTSRFFQNLREMRLTLNSGIVVVATGPPSDYTDEDYV
                     VATEDGDVVIPSQSRIRVYKDTRGEGTTVVVEEGTAAVFSKGNRIDIDEGRMVSVDGE
                     GTFSAVSSAFEDLVSNDRFIDPWTREADRVEHGGLDIAAWTTIREAASATDTRSNSIV
                     VITEVVGSSVNYLVDIKRQGPNDQYGRLGIRQEINRPVDYLHTIELAAVIRVVQQPSI
                     IGGPAGNVYPLTIRVNYTDAQGRAQQWVRSFYYGQDSDVESDPPEVKQVEQNRWTTVT
                     YTLKPNADQPGFEPDIINSIEIYGYGPQFNSSINSISLTGR"
     gene            3547..4860
                     /locus_tag="VFH60_03195"
     CDS             3547..4860
                     /locus_tag="VFH60_03195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET6312816.1"
                     /translation="MSKIAVIGTGYVGLVTGTCFADLGNDVTCVDVVPEKVKMLREGR
                     SPIYEPGLEEIIVRNLRAGRLRFTTSYEEALKGAEFIFIAVGTPESEDGSADMQYVKA
                     AAREIGRVAPGRAHEPMAIINKSTVPIGTGDVVGALVRENAPEDFRFAVVSNPEFLRE
                     GQAVLDFMHPDRVILGSDDREIAERVQELYESLGCPIMITDIRTAEMIKYASNAILAT
                     YISFINEIAFICESLGADVQEVVQGLGYDKRINPAFLNAGVGFGGSCFPKDVKALAHM
                     AETSSARPQLLNAVLDINQEAKRAFVDKVVKLLDGSVKGKRIGVLGLSFKPDTDDMRE
                     SPSIDIVNALSELGADIQAYDPIAMEAASRHLNGKVRFCRDAYETAAGADALLLITSW
                     NEFKALDMDKVRESMRRPVLLDGRNVYDPVEMRELGFTYAGVGRR"
     gene            4869..5333
                     /gene="acpS"
                     /locus_tag="VFH60_03200"
     CDS             4869..5333
                     /gene="acpS"
                     /locus_tag="VFH60_03200"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET6312817.1"
                     /translation="MPDQFVSPSSLAGLSPIAAGLSVADAMDGRLQCGVDLIEISRIQ
                     MALDRWGERFLRRVWTEREIAACRGRHRELAARFAAKEAASKALGTGIVGIVWRELEV
                     LSDRRGKPLLFLHGQARARADELGLNTWAVSITHSRDLACAFVVAYAAPSEA"
     gene            5335..6969
                     /locus_tag="VFH60_03205"
     CDS             5335..6969
                     /locus_tag="VFH60_03205"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HET6312818.1"
                     /translation="MALLVTAAEMRHIEADAVERGQTWEGLMDQAGEAVAHHAISWLG
                     AKTPQRVLVLAGPGNNGGDALVVARHLHDHGWQVRSFTWSRNARPGDRLLAPLGARGI
                     EVQPVAASAPDAGLRNALAWCTVVVDGLLGTGLERDIESPLADIIREVAGARVPVVAI
                     DVPTGIDSDTGGVRGVALKASFTVATGLLKYGHVLEPGKSLSGKIAIGEIGVSQAASR
                     EAAQGTLLTDGDIKKMLPERPKDANKGTFGKAMVVSGSVNYIGAAALATEGAMRSGAG
                     LVTLACAGDLLPILAVKLTECTFLPLPSDLGAIAQAAAEKLFAATKDYNALLVGCGLG
                     KEKETAAFLRSLLAKPEEITHPGMRPIGFAARRAEPKNSEQDKIALPPLVLDGDALNL
                     LADWPEWAEHVPAGSVLTPHPGEMARLTGLTVDEVQADRVGVARENAAKWKQIVVLKG
                     AGTVVAEPGGRVYVSPFSNPGLATAGTGDVLAGTIVGLLAQGLATLDAACAGVYLHGV
                     AGEIMLAEMGPVGGLAGDLARLMPQAQKALREGRDG"
     gene            6962..8233
                     /gene="alr"
                     /locus_tag="VFH60_03210"
     CDS             6962..8233
                     /gene="alr"
                     /locus_tag="VFH60_03210"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET6312819.1"
                     /translation="MVSVGEPAAGQGTEPAAAFQRSGRPLSAEIDLSAIAANTSAIKA
                     LVGPRCRVMAVVKADGYGLGAPWVALAALEGGASWLGVACVDEGVQLRRAGYSGPILV
                     LSYVPPEEAEAAVRNRLTLVLHRERTALALEAAASRLGLPEGKVAVHVKVDTGLGRYG
                     CLPDEFLPLALAIGKLHHVRLQGLMTHFADADNPDLTFAHRQLEIFNDVRARAAEHGI
                     EFEIVHAANSAAALSLPEARFDMVRCGIMLSGHVPSPHLRGQVELKPALTLRSRLARV
                     YEAPQGATVGYGRSWIARSPSLIGLVPLGYADGYRRSLSNRAEALVGGRRVPVVGRVS
                     MDQIGLDLTEIQDAQEGDEVVLVGVQGHEAVTVDDLAEWAGTISYEVLCGLSERVPRR
                     YLHSGEPVEVCDLLGSSPTHDRRMPRKELSR"
     gene            8230..9534
                     /locus_tag="VFH60_03215"
     CDS             8230..9534
                     /locus_tag="VFH60_03215"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET6312820.1"
                     /translation="MRAVDLIAAKRDGREMSREEISWLLKAYQRGDVPDYQLSAWLMA
                     VYFRGMTARETADLTMAMVDSGTRLDLSSLGGFVADKHSTGGVGDKTTLVVAPLVAAA
                     GVPVAKMSGRGLGFSGGTIDKLESIEGFRTQLSAEEFIEVARRTGLVVVAQSPDLAPA
                     DGLLYALRDVTATIESIPLIASSIMSKKIAAGATGVVLDVKVGAGAFMKSLEATRELA
                     TTMRDIGRNVGLQVRAVISGMDQPLGWAVGNALEVQEAVQTLRGEGPDDLLEVSTTLG
                     AHLLNMAGVAGSVEDGVAHLRETLSSGKALAKFREFVEGQGGDWRFIDDFSLLPQAPR
                     QVEVESEEQGYVTAIDAETIGRASVEIGAGRAVKGASIDHSVGFVLHKKLGDTVTPGE
                     SLARVHAASQDAVDLAIRRLRSAFRIAGARVEPMPTVLEVIR"
     gene            complement(9617..>9844)
                     /locus_tag="VFH60_03220"
     CDS             complement(9617..>9844)
                     /locus_tag="VFH60_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312821.1"
                     /translation="EEALRMDAAGDYAGSANRLRTAASYLQDIAPASPTAMAEAQVLE
                     QESKAAEGGFTAITRKAVHYAQSTVRQSRKK"
BASE COUNT         1902 a         2896 c         3287 g         1759 t
ORIGIN      
        1 agaccggata gaaagacccc gtgccaggct tcacgacaaa gcacgggcag gagacacatc
       61 aaaatggcag atactatgaa caacgtaccc ggttcgcccc tggagctaaa gccctacagc
      121 cgccccggcg gcccggcgag acctataagc gcgcatcccg cccccatcga gcgcaaaccc
      181 gaatggctga ccgtcaagtg gcaacagggc gagaactacc gcgacctcaa gggcctcatg
      241 cgagagcttg gcctgcatac cgtgtgcgag gaagcccatt gccccaatat ctcggagtgc
      301 tgggagcacc gcaccgccac gttcctaatt cttggcgaca cctgcacgcg gtcctgcggc
      361 ttctgcgcga taaagacggg ccgcccctcg aagctggacc gccaggagcc ggagcgcgtg
      421 gcgcgggcta tcgaagcctt acagttgaag cacgcggtca tcacatcggt gaaccgggac
      481 gagcttgacg acggtggggc tgagatgttc gccgaagtga tccgcctcac gcgtgaatac
      541 gtgcccggct gcggcctcga ggtgctcata cccgacttca agggggtctg gtgggcgctg
      601 caaagggtta tggacgcgca gcccgacatc ctcaaccaca acgtggagac ggtgccccga
      661 ctttattaca tcgtgcgccc gcaggccaag tacgagcgtt cgctggagct actgcaaaag
      721 gcccgacaga tggacgccca gggccgcagc tttaccaaga gcggcatcat gatcggcctg
      781 ggtgaggaat gggacgagat agtgcagacc atacgcgacc tgcgcgacca cgacgtcgac
      841 atcctcacgg tgggccagta cctcagaccg tccttcaggc acctgcccgt gctcaagtac
      901 tacacgccgg ccgagttcaa ggagctgaag gaaatcgcgt tgggcctggg cttcaagcac
      961 gtggaaagcg ggccgctcgt ccggtcgtca taccacgcgc acgaacaagt gcaggcagcg
     1021 ggcgcataga acggggggcg aacttcatgg ctgacaccat ccagagggcg gagtcgcgga
     1081 ctcgccctct ttctattctc gacctcggtc tcatccctta ttcggaatgc tgggagttgc
     1141 aaaggcgtct tgcgtcggag cgcgccgaag gggcaattgg ggacacgttg ctgttgctcg
     1201 aacacccgca cacctacacc tgcgggaggc gcggcggtcg agaccacatc ctcatcagcg
     1261 aacaggagct gcgcgaacgc ggcatttccg ttctggacgt ggaccggggc ggcgacgtta
     1321 cctatcacgg gccgggccaa ctcgtcgcct atcccatcgt gcgactggca gaagagggcc
     1381 aagtggctga ttatcacgcc tacctgcgag ctttagaaca agttcttata accacattag
     1441 ctgatttcgc aattagggca gatcgcatcg aggggtatag cggcgtctgg gtacgcagtg
     1501 ctgatagcga cgaaaagata gcggccatcg gggtcaaggt agatggacgc ggcgtctcga
     1561 gccacggcat cgccctcaac gtgaacacgg accttcaata cttcagccat atcgtaccgt
     1621 gcggcattgt ggagaaggga gtgacatcca tgcagcgact gcttggcaca gcgcccgaga
     1681 tggccgatgt caagtcagta ttcagccgcg cattctgcgg tgtgttcggc ttctccctcg
     1741 cgctcctgtg acctacgctc gtaccgtgcc ctcgtttgac tacagcatct acgcggcggt
     1801 acaatatcag gcagtggaaa acaatacccc ctctacccta aaggacgctc agtcccaccc
     1861 gccggagctt cagacgctgc acgccacggt gcgcgggcgc gtacagaacg tgggcttcag
     1921 aatgtttgca cgggatgccg cgcaacgcct gggggtggcc ggttacgtgc gcaacgagca
     1981 cgacggcagc gtaacggtcc tggccgtggg agcgcgggag gcactggaga gcttgcttgt
     2041 ggagctccgg cgcggaccgg cccacgcgcg catggagcgg gttgaggcgg actggctgcc
     2101 cggcaacccg cagggcttca ccgggagatt tgaggtgcgt ccctgatgag cgcgagcgtg
     2161 agggccgacg tagtagggac cagcgaccgc gaccgtgcgc gttacgtgtg gcttgtgctc
     2221 gcactcagca cgctagcctt cttcggactg tgcgcgctgg tgggcaacag catattcggc
     2281 ttccttacca gcgtcactga gcggtctgcc gctcggttag agctacgcaa gggcgcacaa
     2341 cttactttgc aacgcttcgg cagcagcgct tcggaggtgg tagccgatga ggcggcgctg
     2401 gaggaggggg accgcacgac caccggcccg ggtagtgagg gctacattga cctgttcggc
     2461 ggcgacatca ccgtctacac ctatttcagt actacagtaa ctatcgacag gctgcggacc
     2521 agccggttct tccagaatct gcgcgagatg cggcttacct tgaacagcgg cattgtggtc
     2581 gtggccactg gtcctccttc tgattacacc gatgaggact acgtcgtggc caccgaggat
     2641 ggcgacgtag tcatacccag ccagtcacgg atccgagttt acaaggacac gaggggcgaa
     2701 ggtaccacgg tcgttgtcga agagggcacg gccgccgtct tttccaaggg caacaggatc
     2761 gacattgacg aaggcaggat ggtcagcgtg gacggagagg gtacttttag cgcggtcagc
     2821 tccgccttcg aggacctggt cagcaatgac agattcatcg acccgtggac gcgcgaggcc
     2881 gatagagtgg agcatggcgg cctcgacatc gcggcatgga cgaccatccg ggaagcggcc
     2941 agcgccacgg atacacgctc gaactccatc gtggtgatca ccgaggtagt tggctcgagt
     3001 gtcaattacc tcgtggatat aaagcgccag ggtccgaacg accagtacgg gcggctcggt
     3061 atccgccagg agatcaaccg ccctgtggac tacctgcata cgattgagct agccgccgta
     3121 atccgggtcg tgcagcaacc gagcatcata ggaggccctg cgggaaatgt ctatcccctg
     3181 acgattcggg taaactatac cgacgctcag gggcgcgccc agcaatgggt ccgcagcttc
     3241 tattacgggc aagattcgga cgttgaaagt gatccgccgg aggtaaaaca ggtcgagcaa
     3301 aatcgctgga cgacggtgac ttacaccctc aagcccaatg ccgaccagcc gggcttcgag
     3361 ccggacatta tcaactcgat tgaaatttat ggctacggcc cgcaattcaa tagctctata
     3421 aactcgattt cgctgacggg gcgttgagca gaggccatac aaccgtaacc gcggccgaac
     3481 gtttgtagag gaggctggta cggagcggaa tggcaaggcg cgagagtaaa ctactggagg
     3541 atagtcttga gcaagatagc ggtaattggc acgggctacg tcggcctggt tacgggtacc
     3601 tgttttgccg acctggggaa cgacgtgact tgtgtggacg tggtaccgga gaaggtcaag
     3661 atgttgcgcg aagggcgctc acccatctac gagcctggtc tcgaagagat catcgtgcgc
     3721 aacctcaggg ccggcaggct gcgcttcacc acctcgtacg aggaggcctt gaagggagcg
     3781 gagttcatct tcatcgcggt aggcaccccc gagtctgagg acggttcggc ggacatgcag
     3841 tacgtcaagg cggctgcccg cgagataggg cgagtcgcgc cgggccgcgc ccacgagccg
     3901 atggcgatca tcaacaagag cacggtgccc atcggcacgg gcgacgtggt aggcgcgctc
     3961 gttcgggaga acgccccgga ggacttccgg ttcgcggttg tctcgaaccc tgagttcctg
     4021 cgcgaagggc aggccgtgct cgacttcatg caccccgacc gcgttattct cggctccgac
     4081 gaccgcgaga tagccgagcg cgtgcaagag ttgtacgagt cgcttggctg ccccattatg
     4141 attaccgaca tacgcaccgc tgaaatgatc aagtacgcct cgaatgcgat actggcgact
     4201 tatatctcgt ttatcaacga gatagccttc atctgcgaga gcctgggcgc ggatgtccaa
     4261 gaggtggtgc agggcctggg ctacgacaag cgcatcaacc cggcctttct caatgcgggc
     4321 gtgggcttcg gtggtagctg cttccccaag gacgtgaaag ccctggcgca catggccgag
     4381 acgagcagcg cccggcccca actgctgaac gcggtccttg acatcaacca ggaagcaaag
     4441 cgcgcgtttg tagacaaggt ggtgaagctc ctggacggct cggtgaaggg caagcgcatc
     4501 ggtgtgctcg gcttgtcatt caagccggac accgacgaca tgcgagaaag cccttctatc
     4561 gatatcgtaa atgccctcag cgagcttggc gcggatatac aagcctacga ccctatcgca
     4621 atggaagccg ccagcaggca cctgaacggg aaggtgcgct tttgcagaga tgcctacgaa
     4681 acggcggcgg gggcggacgc tctgttgctc atcacttcgt ggaacgagtt caaggcgctt
     4741 gacatggaca aggtcaggga gagcatgcgc cggcccgtgt tactcgacgg gcgcaacgtc
     4801 tacgacccgg tggagatgcg agagcttggc ttcacgtacg cgggcgtagg ccgccgttag
     4861 ccggtagcat gccggaccag ttcgtaagcc caagctcgct cgcgggtctt tcgcctatcg
     4921 cggcgggact gtcggtagcc gacgcgatgg acggccggct gcaatgcgga gtggacctga
     4981 tcgagatcag ccgcatccag atggccctgg accgctgggg agagcgcttc ctgcggaggg
     5041 tgtggactga gcgcgagata gccgcctgcc ggggcaggca cagggagttg gccgcgcgct
     5101 tcgccgccaa ggaagccgct tctaaggcgc tgggcaccgg catcgtcggc atcgtgtggc
     5161 gtgagttaga ggtattgtcg gaccggcggg gcaagccgtt gctattcctg catgggcagg
     5221 cgcgggccag ggccgacgag cttggcctca acacctgggc ggtcagcatc acacacagcc
     5281 gcgacctggc ctgcgcgttt gttgtggcat acgccgcgcc ctccgaggca taacgtggct
     5341 ttgctggtca cggcggccga gatgcgccac atcgaggcgg acgcggtcga gcgggggcag
     5401 acctgggaag gtctgatgga ccaggcgggc gaggcggtgg cacatcatgc catcagctgg
     5461 ctaggcgcaa agacccccca gcgcgtgctt gtgctggccg ggccgggcaa caatggtggc
     5521 gacgccctcg tggtggccag gcacctgcac gaccacggct ggcaggtccg gtctttcaca
     5581 tggtcgcgta acgcccgccc cggcgaccgg ttgttggccc cgctgggtgc gagaggcatc
     5641 gaggtgcagc ccgtggccgc cagcgctccc gacgcgggcc tccgcaacgc gcttgcctgg
     5701 tgcaccgtgg ttgtggatgg cctgctaggc accgggttgg agcgcgacat agaatcccca
     5761 ttggctgata taatcaggga agtagccggt gcgcgtgtgc ctgtagtggc cattgacgta
     5821 cccaccggca tcgacagcga tacgggaggg gtgcgcggcg tggcgcttaa agcaagtttc
     5881 acggtcgcga cggggctgct caagtacgga cacgtcttgg agccgggtaa gagtctctca
     5941 ggaaaaatag ctataggaga aatcggagtg agtcaggcag caagccgcga ggcggcacaa
     6001 ggaactctac taacagacgg cgacatcaag aagatgctgc cggagcggcc caaagacgcg
     6061 aacaagggca cgttcggcaa agcgatggtg gtctccggct cggtcaacta catcggagcg
     6121 gcggcattgg ctaccgaggg tgcgatgcgc tccggcgccg gcttggtgac cctggcttgc
     6181 gcgggcgacc tgctgcccat tctcgcggtc aagcttaccg agtgcacctt cttgcctctg
     6241 ccttcggacc tgggggcaat tgcgcaggcg gctgccgaaa agctgttcgc cgcaacgaag
     6301 gactacaacg ccctgcttgt gggctgcggg cttggcaagg agaaagagac agccgcattc
     6361 ttgcggagtc tgcttgccaa accggaggag atcacgcacc ctggtatgcg ccccatcggg
     6421 ttcgctgccc gccgcgcaga accgaagaac agcgagcaag acaagatcgc cctaccgccc
     6481 ctggtgttgg atggcgacgc gctgaacctc ctcgcggact ggccggagtg ggccgagcac
     6541 gtacctgccg gttctgtcct gacgccccac cctggcgaga tggcccggct gacgggtctc
     6601 accgtggatg aagtgcaggc ggacagggtt ggtgtggcgc gggagaacgc ggctaagtgg
     6661 aagcagatcg tggtgctgaa gggtgcgggc accgtcgtag ccgagccggg cgggcgggtc
     6721 tacgtcagcc ccttctcgaa ccccggactg gcaaccgcgg gcaccggcga cgtgcttgcc
     6781 gggaccatag tggggctgct tgcgcaggga ctcgccaccc tggacgcggc ctgcgcaggc
     6841 gtctacctgc acggcgtggc gggcgagatt atgctggcag aaatgggtcc cgtgggaggc
     6901 ctcgcgggag acctcgccag gctcatgcct caagcccaaa aggccctacg agaggggcgt
     6961 gatggttagc gtcggggaac cggctgcggg ccagggtaca gagcctgcgg cagcgtttca
     7021 gaggtcgggg cggccgctaa gcgccgaaat cgacctctcg gctatcgccg cgaacacgag
     7081 cgccatcaag gctcttgttg gtccccgttg ccgcgtaatg gcagttgtga aggccgacgg
     7141 gtacgggctg ggcgctccgt gggtggcgct agccgcgctt gaaggtgggg cttcgtggct
     7201 gggcgtggcc tgcgtagacg agggggtaca gctgcgccgt gcgggctaca gcggtcccat
     7261 cctggtcttg agctatgtgc cccccgaaga ggcggaggcg gcggtccgaa accggctgac
     7321 cctcgtgcta catcgcgagc gtactgctct agcccttgaa gctgccgcgt cccggttggg
     7381 actgcccgag ggtaaagtgg cagtgcacgt caaggtggat accgggttgg gtcgctatgg
     7441 ctgcttgcct gacgagtttc tacctctcgc cctggccatc ggaaagctcc accatgtgcg
     7501 cctgcaaggg ctgatgaccc atttcgccga tgccgataac cccgacctga ctttcgccca
     7561 ccggcaattg gagatcttca acgacgtgag ggcgcgggca gccgagcacg ggatagaatt
     7621 cgagattgtg catgccgcca actctgccgc cgctctgtcc ctgcccgagg cgcggttcga
     7681 catggtacgc tgtgggatca tgcttagcgg gcacgtcccc tcgccccacc tgcgtgggca
     7741 agtcgagcta aagcccgcac taactctgcg ctccaggctg gccagggtgt acgaggcccc
     7801 gcagggcgct actgtaggct atggccggag ctggatcgcc cggtcaccgt cgctgattgg
     7861 cctggtgccc ctgggctatg cggacggcta ccgacgctcc ctctcgaacc gtgctgaggc
     7921 gttggtgggt ggccgtcgcg tgcccgtcgt tgggcgcgtc agcatggacc agatcgggct
     7981 tgacctgacg gagatacagg atgcacagga gggtgacgaa gttgtgctgg tgggcgtgca
     8041 agggcacgaa gccgttacgg ttgacgacct ggcagagtgg gccggtacga tctcttatga
     8101 agtcctgtgc gggctgtcgg agagggtgcc ccgccgctac ctgcactcag gtgagccggt
     8161 tgaagtgtgc gacctcctgg gcagcagccc gacccatgac aggcgcatgc cgcgtaagga
     8221 gcttagccgg tgagagcggt agacctgata gcggccaagc gagacggccg cgagatgagt
     8281 cgggaagaga tctcctggct attgaaagct taccagcggg gagacgtgcc cgactaccag
     8341 ctaagcgcct ggcttatggc ggtctacttt cgtggcatga ccgcacgcga aacggccgac
     8401 ttgacaatgg cgatggtcga ttccggcacg cggctagacc tctcgtcact cggaggattt
     8461 gtggccgaca agcactcgac cggcggggtc ggtgacaaga cgacgctcgt ggtagcccca
     8521 ctggtggccg ccgcgggggt cccggtcgcc aagatgtcgg ggaggggact ggggttttcg
     8581 ggcgggacga ttgataagct ggaatccatc gagggcttcc gcacccaact gtcggccgag
     8641 gagttcatcg aggtggcgcg gcgtactggc ctcgtggtag ttgcgcagtc gcccgacctc
     8701 gctcccgcgg acggcctgct ttacgcgctg cgggatgtaa ccgccacgat tgagagcata
     8761 ccacttatcg cgagcagcat catgtccaag aaaatagcgg cgggtgcgac gggtgttgtg
     8821 ctcgacgtca aggtgggggc aggggcgttc atgaagtcgc ttgaagccac tcgcgagctt
     8881 gctaccacca tgcgcgatat cgggcgcaac gtcggcctac aggtacgggc ggttatctcc
     8941 ggcatggacc agccgctcgg ttgggccgtg ggcaacgcgc ttgaagtcca ggaagcggtg
     9001 cagacactac gcggcgaggg accggacgac cttctggaag tctctaccac gctgggcgcg
     9061 cacctgctga acatggcggg cgtggcgggt tctgtcgagg acggagtggc ccacctgcgc
     9121 gagaccctta gctcaggcaa ggccctggca aaatttcgcg agttcgttga aggccagggc
     9181 ggcgactggc gcttcataga cgacttctcc ctcctcccgc aggcacctcg tcaggtcgaa
     9241 gtcgaatcgg aagagcaggg ctacgtcacg gccatagatg cggagaccat cgggagagcc
     9301 tcggtcgaga ttggcgcggg tagagcggtc aagggtgctt ccatcgacca ttcggtgggt
     9361 ttcgttctac acaagaagct aggcgacaca gtcacgccgg gtgagagcct tgcaagggta
     9421 catgccgctt cccaggacgc cgtggacctg gccatccgcc gcctgcgtag cgcgttccga
     9481 atcgccggcg cacgtgtcga gcctatgccc accgtgctgg aagtgatacg gtagaggaac
     9541 gggaatttag gatctaggat ttagcatttt ggaggtttcg ggaaaaccta aatcctaaat
     9601 cctaaatgtc ctccttctat ttctttctgc tttgacgaac ggttgactgc gcgtagtgga
     9661 cggctttgcg ggtgatggcg gtgaagccgc cctccgccgc tttgctttct tgctcaagta
     9721 cctgtgcctc cgccatcgcg gtgggtgatg cgggagcaat atcttgcagg tacgaagctg
     9781 ccgtgcgcaa tcgatttgcg ctgccagcat agtctcccgc ggcgtccatc ctgagcgcct
     9841 cttc
//
LOCUS       DASRWZ010000209         6251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1181972, whole genome shotgun sequence.
ACCESSION   DASRWZ010000209 DASRWZ010000000
VERSION     DASRWZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181972"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VFH60_03225"
     CDS             complement(<1..788)
                     /locus_tag="VFH60_03225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET6312822.1"
                     /translation="MYVKNDMLGHFGRVAEEAARTAGSLALSGFRGEMQVRTKGRKDI
                     VTEYDTAAEDEAVRIVKGDFPEHGILAEESGASYLEDAAPRYLWAIDPIDGTHNYAMQ
                     LPYWCCSVAVLDSIERVVLAAAVFDPLHGEMFVARAGEGAYLNGRPLRVAALDKLEDA
                     TLAFDIGYDPAISGRMLNITTRLQPQVRRVRLLGSAVLALTYVAAGRFDAYYHLHLMP
                     WDIAAASLLIKEAGGVVTDWEGNPAGTSGSGVAAANPALHPQLR"
     gene            complement(795..1970)
                     /locus_tag="VFH60_03230"
     CDS             complement(795..1970)
                     /locus_tag="VFH60_03230"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET6312823.1"
                     /translation="MNTLPEQNPYDVLQVPRDASIDEIEDAYDALYDLHEPGARAGDE
                     REIAFLTDLNEAREVLLDPQRRSHLDQKLHERTRRPTKAPAPRTSPYTRPQEKSSARP
                     AHPSPGAMAPTTGTVPAKVRRRSGPRPRYVAPPPTRDRRVPLLVGGLFMVVLVFALAV
                     FLMMRGQQGGLAVTPDPARGEVIASVNGVPVYEDDWKERIERDKNSALSDPLFSPLVN
                     NFQGITGTRMLDILSYDAMDKLINLEVIMQQAKKEGLYPTPAQERGLIDEAKGRDLQG
                     GKSFEQFLSERNLTEGKYNRTVIQNVVYTVMADRHLPPTGTSQARTDGFIKWICETRK
                     SYDVRINRKFMLESNVPCTSGLPSDLPLPGIEQEEIPDEIATAVVPASPNPNSTPAP"
     gene            complement(2007..3818)
                     /gene="lepA"
                     /locus_tag="VFH60_03235"
     CDS             complement(2007..3818)
                     /gene="lepA"
                     /locus_tag="VFH60_03235"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET6312824.1"
                     /translation="MPEQRLVRNFCIIAHIDHGKSTLADRLLEITGTVAQRNMVAQML
                     DSMELEREKGITIKARAVRMSYKADDGERYELNLIDTPGHVDFTYEVSRSLAACEGAL
                     LVVDATQGIEAQTLANTYLAMEHDLALVPVVNKIDLPAADPDTVAAEIENVIGLPNEE
                     IIRASAKEGTGVKEILEAVVKHIPPPKGNPSAPLRALVFDSHYDAYKGVIAYVRIVDG
                     EISTGDNLLVMSSGVVTEAQELGSFTPEMTPTNKLTTGEVGYIATGLKIVRDCQVGDT
                     ITLSRRASAEPLPGYKAIKPMVFAGLYPVESDDYVELREALERLHLNDASLVYQPESS
                     AALGFGFRCGFLGLLHMEIVRERLEREYDLSLLITAPSVEYQVTRTDGQIVTVDNPSE
                     MPDPGKIATIEEPWMEISVVTPSRYIGPIMELVTTRRGIYKKMDYLDEERVVLGYDIP
                     LGELIVDFYDQLKSRTQGYASLDYQFAEYQAADLVKLDILVNGQPVDALSIITHRDKA
                     HYQGRQLVDRLRRLIPRQLFEVPVQAAIGGKVVARETISAIRKNVLAKCYGGDISRKR
                     KLLEKQKEGKARMKMVGNVEIPQEAFMAVLSLEEPVK"
     gene            complement(4349..4603)
                     /locus_tag="VFH60_03240"
     CDS             complement(4349..4603)
                     /locus_tag="VFH60_03240"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6312825.1"
                     /translation="MEILGRVTDSEGEPVRDAVVLILSGTASHPDIAALTDEQGKYRL
                     TGLAPGTYRVAVNAADRPPASSEVHLNSAEGAQLDFRLSR"
     gene            complement(4635..5864)
                     /locus_tag="VFH60_03245"
     CDS             complement(4635..5864)
                     /locus_tag="VFH60_03245"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET6312826.1"
                     /translation="MPKAGNSRNVKPEGAEADGGFVEGLAPNSRPSGVSTSTARSITE
                     VDEVDPRDDEPVSSPNLNELLIAGAGEAERESTDRGELEDVAGYAHTHGFEAAAGQRS
                     IYRVGGSTYGDTIEASVPFNGQSLSVEVPLLDAWYAQYASPTERAAALRASATSVLRE
                     QARTNSGPALLGMGPEASYVSPDIQEVVIGRDDRVRINNTKVVPWQWICSLLMTAGDG
                     TRWIGTGWLIGPRTVITAGHCVYMREHGGWVREMQVMPGRNASDSSLGNVVANQFRSV
                     RGWVEGQKRICDYGAIILPESSPFGRQLGTFGYAMPADDVLAGLLVNISGYPGDKPPG
                     TQWYHSRTLTSITARTLVYDVDTAGGQSGAPVWWRQGDKRYVVGIHTNGATSGNSATR
                     IVQAVHQNLTAWRDEGA"
BASE COUNT         1058 a         1941 c         1943 g         1309 t
ORIGIN      
        1 tgccgcagtt gtgggtgcag ggctggattg gcggctgcta caccgcttcc cgacgtaccc
       61 gctgggttgc cttcccagtc ggtgacgaca ccccccgctt cctttatcaa gagggaggcg
      121 gccgcgatat cccagggcat caggtggagg tgatagtacg catcgaagcg cccggctgcc
      181 acgtaggtca gggcgagcac ggctgagcct agcaatcgca cccggcgcac ctgcggctgt
      241 aacctggtgg tgatgttcag catgcggcct gaaatagcgg ggtcgtaccc gatgtcgaaa
      301 gccagggtgg cgtcttctaa cttgtcgagg gccgctaccc gcaggggccg cccgttaagg
      361 tacgcacctt ctcccgccct ggcgacgaac atctcgccgt gcaacggatc gaacacggca
      421 gccgccagca ctacgcgctc gatggagtct aggacggcca ccgagcagca ccagtaggga
      481 agctgcatgg cgtagttgtg cgtcccatct atggggtcga tggcccacag gtagcgaggg
      541 gccgcgtcct caaggtagct ggccccactt tcttccgcga ggatgccgtg ctccgggaaa
      601 tcgcccttta cgatacgcac cgcttcgtcc tcggcggcgg tgtcgtactc ggtcacgatg
      661 tccttgcggc ccttggtgcg cacctgcatt tcaccccgga agcccgacag cgcgagcgat
      721 cctgccgtcc tggccgcttc ttccgcgacc cggccaaagt gccccagcat gtcgttcttg
      781 acgtacatgt agggtcaggg cgcgggtgtg gagttcgggt tcgggctggc ggggacgacg
      841 gcggtggcta tctcgtccgg tatctcttcc tgttctatac cggggagggg caggtcgcta
      901 ggcagaccgc tggtgcacgg tacgttcgac tccagcatga acttcctgtt gatacgaacg
      961 tcgtaggact tgcgtgtctc gcaaatccac ttgatgaagc catcggttcg cgcttgcgag
     1021 gtgcctgtag gcggcaagtg cctgtcggcc atgaccgtgt acaccacgtt ctggatgacg
     1081 gtgcggttgt atttgccttc ggtgaggttt ctctcactca gaaactgctc gaaactcttg
     1141 ccgccctgca agtccctccc cttggcctcg tctatcaggc ctctctcctg ggcgggagtg
     1201 ggatagagac cctccttctt agcctgctgc atgatgactt cgagattgat gagcttgtcc
     1261 atagcgtcat agctcaggat atccagcatg cgggtgcccg tgataccctg gaagttgttc
     1321 acgagagggg agaagagtgg gtcggatagg gccgagttct tgtcgcgctc gatacgctcc
     1381 ttccagtcgt cctcataaac cggtacgccg ttcacgctgg caatcacttc accccgcgcc
     1441 gggtccggcg taacggccag gccgccctgc tgcccgcgca tcatcaggaa cacggcgagg
     1501 gcgaacacga gcactaccat gaacaaaccg ccgaccagta gaggcacgcg ccggtcgcga
     1561 gtagggggtg gtgccacgta ccgggggcga ggcccggacc tgcgccttac cttcgccggt
     1621 accgtgccgg tggtgggggc cattgcgccg ggggaggggt gagccggccg ggccgacgac
     1681 ttctcctgcg gcctagtgta aggggaggtg cgcggggcgg gggccttggt gggccttctc
     1741 gtccgttcgt gtagcttctg gtccaggtgc gagcggcgct gagggtccag caggacttcg
     1801 cgcgcctcgt tcaggtcggt gaggaaggct atctcgcgct cgtcaccggc gcgcgcccca
     1861 ggctcgtgca ggtcgtagag cgcgtcgtag gcgtcctcaa tctcgtctat gctggcgtcg
     1921 cgtgggactt gcagcacgtc gtagggattt tgttcgggca gggtgttcac ggcctcacgt
     1981 tctccactag aattgcgccg gcctacttac ttgaccggtt cttccaacga tagcaccgcc
     2041 atgaaggctt cctgcggtat ctcgacgttg cccaccatct tcatgcgggc cttgccttct
     2101 ttttgcttct ccagcagctt gcgcttgcgc gaaatgtcgc cgccgtagca cttggcgagc
     2161 acgttcttgc ggatagccga aatcgtctcg cgggccacca ccttgccgcc tatcgccgcc
     2221 tgtacgggca cctcgaatag ctggcgcggg atgagcctgc gcaggcggtc cacgagctgc
     2281 cgcccctggt agtgggcctt gtcgcggtgc gtaattatgc tcaacgcatc cacgggctgg
     2341 ccgttcacca ggatgtcgag cttcaccagg tcggcggcct ggtactcggc gaactggtag
     2401 tccagcgagg cgtagccctg ggtgcgggac ttcaactggt catagaagtc cacgataagc
     2461 tcgcccaggg ggatatcgta gccgagcacc acgcgctcct cgtcgaggta gtccatcttc
     2521 ttgtagatac cccggcgggt ggtcacaagc tccatgatgg ggccgatgta gcgcgagggc
     2581 gtgaccacgc tgatttccat ccagggttct tcaatcgtgg cgattttgcc ggggtcgggc
     2641 atctcgctgg ggttgtccac cgtaactatc tgcccgtcgg tgcgcgtgac ctggtactcc
     2701 acgctgggag cggtaatcag cagcgagagg tcgtattccc gttccagccg ctccctgacg
     2761 atttccatgt ggagcaggcc caggaagccg cacctgaagc cgaagcccag cgccgcgctg
     2821 ctttcgggct ggtagaccag ggaagcatcg ttcaggtgca gacgctccag agcttcgcgc
     2881 agctcaacgt agtcgtcgct ctccacgggg tagaggcccg cgaacaccat cggcttgatg
     2941 gccttgtagc ccggcagcgg ttcggcggag gccctgcgcg acaaggtgat ggtgtcaccc
     3001 acctgacaat cacgtacgat ctttagccca gtggctatgt agccaacttc gccggtggtc
     3061 agcttgttcg taggcgtcat ttcgggcgtg aacgagccga gttcctgggc ctcggtgacc
     3121 accccactcg acataacaag caaattgtcg cccgtgctga tctcgccgtc cacgatgcgc
     3181 acgtaggcaa ttacgccctt gtaggcatcg tagtggctgt cgaacaccaa ggcgcggagg
     3241 ggagcactgg ggttgccttt gggcggcggt atgtgcttga ccaccgcttc aagtatctcc
     3301 ttgacgccgg tgccttcctt cgcggaggcg cggatgatct cctcgttggg caggccgatg
     3361 acgttctcta tctcggcggc caccgtatcg gggtccgcag caggcaggtc gatcttgttg
     3421 accacgggca ccaacgccag gtcgtgctcc atcgccaggt aggtgttggc gagcgtctgc
     3481 gcctcgatgc cctgggtcgc gtccacaacc aggagagcgc cttcgcaggc cgcgagggag
     3541 cggctaactt cgtaagtgaa gtccacatgg ccgggagtgt caatcaggtt cagctcgtac
     3601 cgctcgccgt cgtcggcctt gtacgacatg cgcacggcgc gcgccttgat ggtgatgccc
     3661 ttctcccgct ccaactccat agagtcgagc atctgggcca ccatgttgcg ctgagcgacc
     3721 gtgccggtga tctccagcag gcggtcagcg agggtagatt ttccgtggtc tatgtgggcg
     3781 attatgcaga aattgcggac taatcgttgt tccggcatct agtgtatggc ttcccagtga
     3841 ataaggtgga aggcaaaaaa gaacgctctt gcgagcgcgt ccgattcgag gtctcacctg
     3901 tcagtataga aaggcagggg ccgctttgtc aagcgtgacg tgacacgggc ccgcagccga
     3961 gccattcagg tgggcaagga gccggtgtgc cctggcaggg atatactgcc gcgctgccct
     4021 gccagtgaat ttaccggcta acttgcagcc aggtctccct caaaggagaa tagcgcaggg
     4081 cagtgagtat ccgcagggga cttcgctagc tgccagcccg cggcaaatga agattgataa
     4141 gttgataagg tagcggcctg taggggcagg cccccgtgcc tgccctggtg gtacgcgtac
     4201 aatgttgcta ttctccaaca agggcaggca cgggggcctg cccctacgta gcacccattc
     4261 gtgcccggcc attacctgac taactcgttc ttcttcatca gccacagggc aaccccaggg
     4321 tctccccgcg aggccctgca ctcggcggtc acctcgacag cctgaagtcc agttgcgcgc
     4381 cttccgccga gttgaggtgc acctctgagc tagcgggggg gcggtctgcc gcgttcaccg
     4441 cgaccctgta ggtgccggga gcgaggccgg tcagccggta ctttccctgc tcgtcggtga
     4501 gtgcggctat gtcggggtgg gatgccgttc cagagaggat cagtacaacg gcatcccgca
     4561 ccggctcgcc ctctgagtcc gtaacacggc ccaggatttc catgttcttg cttcccttca
     4621 gtaagagggt ggggctaggc cccctcgtcc cgccaggcgg tgaggttctg gtgcacggcc
     4681 tgcacgatgc gggtcgcgga gttgccggag gtagctccgt tcgtgtggat gccgaccacg
     4741 tagcgcttgt ctccctgccg ccaccagacg ggggcgccgc tctgcccacc tgccgtatct
     4801 acgtcgtaaa ccagcgtgcg cgcagttatg gaggttaggg tgcggctgtg ataccattgc
     4861 gtgccggggg gcttgtcgcc agggtagccg ctgatgttca ccagaagccc tgccagcacg
     4921 tcatctgccg gcatcgcgta gccgaaggtc ccaagctgcc gcccgaacgg gctgctctca
     4981 ggcaatatta tggcaccata gtcgcatatg cgcttctgcc cctcgaccca cccacgcacg
     5041 ctgcgaaact ggttggcaac gacgttgccc aggctgctgt cggacgcgtt tcgccctggc
     5101 atgacctgca tttcgcgcac ccaacccccg tgctctcgca tgtagacgca atggcccgcg
     5161 gtgataacgg tccgggggcc gataagccag ccggtgccta tccagcgtgt cccgtcgccc
     5221 gccgtcatta acagcgagca gatccactgc caggggacga ctttcgtgtt gttaatccgc
     5281 actcggtcgt cacggccgat aaccacctcc tggatgtccg gagagacata cgacgcctcc
     5341 ggccccatgc caagcaaggc cggtccgcta ttggtacgcg cttgctcgcg aaggaccgag
     5401 gtcgcgctgg ctcgcaacgc tgcggctcgt tcggtcgggc tggcgtactg ggcgtaccac
     5461 gcgtcgagca acggcacttc aacagatagc gactgtccgt tgaagggtac gctggcttcg
     5521 atggtgtctc cgtaagtgct gccacccaca cgatagatag agcgctgccc cgcggcggcc
     5581 tcgaagccgt gagtgtgcgc gtaaccggcc acatcttcaa gctcgccgcg atctgtactc
     5641 tccctctccg cttcccccgc gcccgcgatg aggagttcgt tcagattggg gctggatacc
     5701 ggctcatcgt ctcgcgggtc cacttcatcc acttccgtga tgctcctggc cgtagaggtg
     5761 ctcacccctg acgggcgcga gttgggcgca agaccttcaa caaagccgcc gtctgcctcc
     5821 gccccttctg gcttcacgtt gcggctgttt cctgctttcg gcatttctat acctcctgat
     5881 tcggaacgct tctcttgctc cgttgactgt tatagctcgc gcataggggg aagcctgcac
     5941 actgtggtcc tatcttgtct ttttcacaca ggtgaattct cctcccgaat acactatatc
     6001 aaaggctggt gcgcgatgtg cgtcacaaaa agtgtcacag aacgaaacgc cctggcaaag
     6061 tgtagaaacg atatcatggc tgatagcttg ctaatagcac tcacgtctcg ctactttgtt
     6121 tttgagatag tttccggcac cggcagccgt cccattagta tggctggcgg tgcacaactc
     6181 tacccataag cgccggttct tgctaaaatc cccattggcg agcggagcac gcacgttccg
     6241 cagcctttgc a
//
LOCUS       DASRWZ010000210         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_976100, whole genome shotgun sequence.
ACCESSION   DASRWZ010000210 DASRWZ010000000
VERSION     DASRWZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976100"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="VFH60_03250"
     CDS             <1..1223
                     /locus_tag="VFH60_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6312827.1"
                     /translation="VIKRSGKQSLVLGVRRTGEVLGEMSLIEQVPRMATVRALRDSQV
                     LGISQNGFHQLIAGNPQAVNAILRTFITRLRSTESLLVQNEKMASLGTLAAGLAHELN
                     NPAAAVRRSSDQLKEVFAEWQRLAAEIDLLDLGPDRSRKLAELRTGMDKRAATNVMLD
                     PLTRSDREDELQTWLEDHDVDQAWELAPTLVSFGWEPGELEELAGAFSDEQLPVIVRW
                     LAAGNSAFSLLDEVSKGATRISEIVKAVKTYSYLDQAPIQEVDVHEGLENTLVILRHK
                     LKNVKVNREYDPDLPKIEAYASELNQVWTNLIDNAIDAMKGEGEITLRTYARPDSVVV
                     EIADSGPGIPEEVQQRIWEPFFTTKAPGVGTGLGLHIVYNIIADKHRGQARVESRPGM
                     TCFQVTLPLKLARE"
     gene            complement(1554..2927)
                     /locus_tag="VFH60_03255"
     CDS             complement(1554..2927)
                     /locus_tag="VFH60_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6312828.1"
                     /translation="MSDLESVFAWIDSQRDAAITDLQRYCRQPSIAAQGVGMEEMAAI
                     VAADLRDLGADTTLVPTAGYPVAVGRLDGDSPRRVMVYNHYDVQPPEPLEEWRTPPFE
                     AAIEDGVLYARGVADNKGNVVARMWAARAWLAVRGSLPCGVTFVFEGEEEVGSPHLAD
                     FAAANRALLEADACLWEAGYREPNGSLALYAGLKGMLYVELRARTVAYDLHSGNANLA
                     PNAAWSLVRALHSLRDDGGRVLIPGFYDGVRPPSERDLALIRQSPMDVQSTAQAWGVN
                     RLLGPSQDPVDLTVQSLFEPTCNIAGIWGGYSGPGTKTVLPATAGAKIDFRLVPDQDP
                     ERILTSLRAYLADKGFSDVEVVALEGTEWPAQSPVETPLMEAMVRAARTVYGSEPRIV
                     TRMAGTGPMEQLCQRHGLPAVGGAGVGNSNSRVHAPNENVRLEDFVLGIKHVAAMLAE
                     FAALHEA"
BASE COUNT          599 a          991 c         1093 g          551 t
ORIGIN      
        1 aagtcatcaa gcgctcgggt aagcaaagcc tggtgctggg ggtgcgcagg acgggcgagg
       61 tattggggga gatgtcgctg atagagcagg tgccccgcat ggcgacggtg cgcgcgctca
      121 gggacagtca ggtgctgggc atcagccaga acggcttcca ccagcttatc gcgggcaacc
      181 cgcaggcggt gaacgccatc ctgcgcacct tcatcacccg cctgcgcagc accgagtcgc
      241 tgctggtgca aaacgagaag atggcctccc tcggcacgct ggcggcgggg ctggcccacg
      301 agctgaacaa ccccgccgcc gccgtgcgcc gtagctccga ccagctaaag gaggtgttcg
      361 cggagtggca gcgcctggca gccgaaatcg acctgctcga cctcggcccc gaccggtcgc
      421 ggaagctggc ggagttgcgc accggcatgg acaagcgcgc cgccaccaac gtgatgctcg
      481 accccctcac ccgtagcgac cgagaggacg agctacagac ctggctggaa gaccacgacg
      541 tagaccaggc gtgggagctt gcccccacgc tggtgtcgtt cggctgggag cctggcgagc
      601 tggaggagct tgcgggtgcg ttctcggacg agcagttgcc ggtaatcgtg cgctggctgg
      661 cggcgggcaa ctcggccttt tcgctgctgg atgaagtgag taagggggcg acccgcatct
      721 cggagatcgt gaaggcggtc aagacctact cctacctgga ccaggctccc atccaggagg
      781 tagacgtgca cgaggggctg gagaacacgc tcgtcatcct gcggcacaag ctgaagaacg
      841 tcaaagtcaa ccgcgagtac gaccccgacc tgccaaagat cgaggcctac gccagcgagc
      901 taaaccaggt gtggaccaac ctgatagaca acgcgattga cgcgatgaag ggagaagggg
      961 agattacact gcgcacctac gcccggccgg acagcgtggt ggtggagatc gcggacagcg
     1021 ggcctggcat accggaggag gtgcagcagc gcatctggga gccgttcttc accaccaagg
     1081 ccccaggcgt gggcaccggg ctgggcctgc acatcgtcta caacatcatc gcggacaagc
     1141 accgggggca ggcccgcgtg gagtcgcgcc ccggcatgac gtgcttccag gtgacgctgc
     1201 cgctgaagct ggcgagggag taagcatcta tctcttgggt atggtgctcc gcatgacgcc
     1261 ctgtggctga agccacgggc tgagaagtag cgaagtctcc ttcggagact ggggcttacc
     1321 tgcaaagtgc cggccgcgtt ggactaaaga tggcccctgt cattctgagc gcagcgaaga
     1381 atctcgtccc tcaccgaggt gttccacctt tcttcccgta cacaggggaa ttattctgtg
     1441 ctacaagaaa ggtgacattt gggtgctgga ggacgagatt cttcgctgcg ctcagaatga
     1501 caaagggata cctggcacga taggttcaac atatgggcta tccacaaggc gacctatgcc
     1561 tcatgcaaag cagcaaactc tgcaagcata gccgccacgt gcttgatgcc gagcacgaag
     1621 tcctccagcc tgacgttctc gttgggggcg tggacgcggg agttggagtt gcccacgccc
     1681 gcgccaccca cggcgggcag accgtgacgc tggcatagct gctccatcgg ccccgtgccc
     1741 gccatgcgcg tgacgatgcg cggctcgctg ccgtagacgg tgcgggcggc ccgtaccatc
     1801 gcctccatca gtggggtctc tacggggctt tgcgcgggcc actcggtgcc ttcgagggcg
     1861 accacttcca cgtcggagaa gcctttgtct gccaggtagg cgcgcaggga ggtgaggatg
     1921 cgctccgggt cctggtcggg caccaggcgg aagtcgatct tggcaccggc ggtagcgggg
     1981 agcaccgtct tggtgccggg gccgctgtag ccgccccaga tgcccgctat gttgcaggtc
     2041 ggctcgaaca ggctctgcac tgtgagatcg accgggtcct ggctaggccc caggaggcgg
     2101 ttaacacccc aggcttgcgc ggttgactgc acgtccatcg gggactggcg tatcagggcg
     2161 aggtcgcgct cggaaggggg gcgcacgccg tcgtaaaagc cgggtatgag caccctgccc
     2221 ccgtcgtcgc ggagggagtg caaggccctc accaggctcc acgcggcgtt gggcgcgagg
     2281 ttggcattgc cggaatggag atcgtaagcg acggtgcggg cgcgcagctc cacgtagagc
     2341 atgcctttca gcccggcgta cagggcgagc gacccgttag gctcccggta gcccgcctcc
     2401 cacaggcagg cgtccgcctc cagcagcgcg cgattggcag ccgcgaaatc ggccaggtgg
     2461 gggctgccca cctcctcctc tccctcgaac acgaacgtca ccccgcaggg tagcgagccg
     2521 cgcaccgcta gccaggcacg cgccgcccac atgcgggcca ccacgttgcc cttgttgtcg
     2581 gccaccccac gggcgtagag caccccgtcc tctatcgcgg cctcgaaggg gggcgtgcgc
     2641 cactcctcaa gcggctccgg tggctgcacg tcgtagtggt tgtagaccat cacgcggcgg
     2701 gggctgtcgc cgtcgagcct gccaaccgct acagggtagc ccgcggtggg cacgagcgtg
     2761 gtgtcggccc ccaggtcgcg caggtcggcg gcgacaatgg cggccatctc ctccatgccc
     2821 acgccctgcg cggcgatgct gggctggcgg cagtagcgct gcaagtcggt gatggcggcg
     2881 tcccgctggg aatctatcca ggcaaacacg gattcaaggt cggacatggg gcctccggat
     2941 ttggaaattg gaatttcgga tttcggattt gcttagtatt ctactaagaa ctaagggcag
     3001 cttacaaaat tggttgggcg aggagtgggg agtaacaggt tatgctgcac cactcaggag
     3061 attgtttgtc attccgagcg tagcgaggaa tcaggtgggt agggaagaag tcgcattaca
     3121 gtactgagta gcggtgtgcg tttgccactc gcatgctgca attcgactgg gtacgcctgc
     3181 cacctgattc ctcgctacgc tcggaatgac aaacacttct ttcggtcctg cgtt
//
LOCUS       DASRWZ010000211         3783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_702153, whole genome shotgun sequence.
ACCESSION   DASRWZ010000211 DASRWZ010000000
VERSION     DASRWZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702153"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VFH60_03260"
     CDS             <1..870
                     /locus_tag="VFH60_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312829.1"
                     /translation="GVVDGAVFNANGDLLLYRVCGELDREGSYVCRLRLVDVALQELH
                     PNTVATITVRGRDMQDWDLTGGFTRDGRHVLLVEKSREETIVRLYTPNSGDAWTLNDE
                     ASHMLAAALSNDALVPLGVPGLSRWTGKNALSHIGGAIPLLEARGLWPQRYFGGGYWV
                     AASPSDRYLLYLDADDTAPAGRSTRGSPATYSLYSAPFRSNVPGDDRAAARRLLSTTL
                     LPHAWLPSIYMLPEGKTLLTTAPPQPHDRPGLYAYDLQTGEHVLAIPGVTDLWRPGYY
                     TLQPQMPVFDPGE"
     gene            complement(889..2514)
                     /locus_tag="VFH60_03265"
     CDS             complement(889..2514)
                     /locus_tag="VFH60_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6312830.1"
                     /translation="MQHQPSGPQSSSLITHYSLRDTLLGLVLVALLALAIVASWKNPE
                     VEGRTRVRQAYGLLDEGRYTRAVARLEETLLLYDEPEARLALSYAYLARRDLERAERQ
                     ARLVLDAGRPDVLPAGWTQLGRVLQAAGQPEDALAAWNMAVRTARPYRGIPRIEADVR
                     SAMWNVAMFHWARGEFDAARGVLQDLAAGDDTYGLSARVQLAQLLGPSEGERALQLLE
                     SIPPPGPSPTPGAFNPQTRVPTTPNLRVPGLSEGLTPGQRDGLVVTVRSALESARNTH
                     AGDGRFESLMVWGNAYLLQGEPRLARLQLAQAVAMDPESSEGHAQYGLALAGVGEVEG
                     AIRELETATALDPRLPLPHNALARLSMQGGRWDDALREIDALQRLQATSATSHLLRGE
                     YHRLRGEYSRAEEALFQAAAIQRGLGAQPGEPDAQLTLALFYIDVVGEGCLKGLPAAQ
                     GSVAAHPDHPGSLDAVGWAFALCGRPNEALPPLEEAVSRAPDHPRYRFHLGRAYAQLG
                     RDADAREQYTRVLDFDPGGSWTNLAVTELAKLP"
     gene            complement(2528..3640)
                     /locus_tag="VFH60_03270"
     CDS             complement(2528..3640)
                     /locus_tag="VFH60_03270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6312831.1"
                     /translation="MAEYQKPVQELAGYPDLDVTLMLPRRWPERAGQMVRAETLPGAS
                     FRVIVARTAFTGLYYIYFFPTLLFHLLRLRPDIIYCYEEAHTLMAALVLLLRRIFLPR
                     TRMLLYAAQNIKKQYPLPFRLFERYAFRNTDMILACGTTVAMTLRSKGYRGSLRVVPL
                     PTDACAFAPDPARREAQRRELGIPQDGLVLLYAGKLVEEKGLLSLWRAFEEVAAEQER
                     ACLVLAGGGPLKSKLEEKAREAGLESRVRLPGVVHNTQLPNMMNAADVFVLPSETRPN
                     WREQFGRVAVEAMSCALPVVGSDSGEIPTVLGDAGLIFPEGDSAALASHLRRLLSDAP
                     LRAQLGSRGRERVLSLFTTEKVAAQHHNVYRVMSDE"
BASE COUNT          611 a         1286 c         1270 g          616 t
ORIGIN      
        1 ggggtcgtgg atggggcggt tttcaatgcc aacggcgacc tcctgctgta ccgggtgtgc
       61 ggggaactcg accgggaggg cagctatgtg tgccggttgc gcctggtgga cgtggcccta
      121 caggagctac accccaacac ggtcgctacg ataacggtgc gcgggcgcga tatgcaggac
      181 tgggacctta cgggcgggtt cacgcgggac ggcaggcacg tgctgctggt tgagaagtcg
      241 cgcgaagaga ccatcgtgcg cctgtacacg cccaactccg gcgacgcctg gaccctgaac
      301 gacgaggcca gccacatgct cgcggcggcc ctctccaacg acgcgctggt gccgctggga
      361 gtgcccggcc tctcgcgctg gacgggcaag aacgcgctca gccatatagg gggtgctatc
      421 cccctcctcg aagcacgtgg cttgtggccg cagcgttact tcggcggggg ctactgggtg
      481 gccgccagcc cgagcgaccg ctacctgctc tacctcgatg cagacgacac ggcaccggcg
      541 ggccggagca ccaggggaag ccctgccact tacagcctct actctgcccc gttccgctcg
      601 aacgtgccgg gcgacgacag ggcagccgcc cgccggctgt tgagcacgac gctgctcccg
      661 catgcgtggc taccctccat ttacatgctg ccggagggta agacgctcct caccaccgcg
      721 ccgccccagc cccacgaccg tcccggcctc tacgcctacg accttcagac aggagagcac
      781 gtgctggcta tccccggcgt gacagacctc tggcggcccg gctactacac gctgcaaccc
      841 caaatgccgg tgttcgaccc cggcgagtga cccggcgcgg gtaagcggtc agggcagttt
      901 cgcaagctcg gtcacggcca ggttggtcca cgagccgccg gggtcgaagt ccagcacgcg
      961 ggtgtattgc tccctcgcgt ctgcgtcgcg gcccaattgc gcgtaggccc tgcccaggtg
     1021 gaatcggtag cgcgggtggt cgggggcgcg ggacacggcc tcttccaggg ggggcagggc
     1081 ttcgttgggc ctgccgcaca gggcaaaggc ccagcctacc gcgtccaggg acccggggtg
     1141 gtcggggtgc gcggctaccg acccctgcgc ggcgggcagg cccttgaggc agccttcgcc
     1201 tactacatcg atatagaaga gggcaagggt aagctgggcg tccggctctc cgggttgcgc
     1261 gcccaggccc ctttgtatgg ccgccgcctg gaacaacgcc tcctcggccc ggctgtactc
     1321 cccccgcagc cggtggtact cgcccctgag caggtgcgag gtggcgctgg tggcttgcag
     1381 gcgctggagg gcgtctatct cccgcagcgc atcgtcccac cgtcccccct gcatggagag
     1441 gcgggccagg gcgttgtgcg gcaggggcaa gcgcgggtcg agggccgtgg cggtctcaag
     1501 ctccctgata gccccctcaa cctcccctac gcccgccagc gcgagaccat actgcgcgtg
     1561 cccctcgctc gactccgggt ccatagcgac cgcctgcgcc agttgcagcc tggcaagcct
     1621 cggctcgccc tgtagcaggt acgcattccc ccataccatc agactctcaa atcgaccatc
     1681 accagcgtgc gtattccttg ccgactccag cgcgctcctg acagtaacca ccagtccatc
     1741 cctttggccg ggcgttagcc cctcgctgag acccggcacg cgcaggttgg gcgtcgtggg
     1801 cacccgcgtt tgggggttga acgcacctgg cgtgggggat ggccctggtg ggggtatgct
     1861 ctcgagtagt tggagggcac gctcgccctc gctggggccg agtagctggg ctagctgcac
     1921 cctggcgctc aggccgtatg tgtcatcgcc cgccgccagg tcttgcagca cgccgcgcgc
     1981 cgcgtcgaac tccccccgcg cccagtggaa catcgccacg ttccacatag ccgagcgcac
     2041 gtcggcctcg atacgcggga tgccacggta gggccgggct gtccgcacgg ccatgttcca
     2101 ggcggccagc gcgtcttcgg gctgacccgc ggcctgtagc acgcggccca gttgcgtcca
     2161 cccggcgggg agcacgtccg gccgccccgc atcgagcacc agccgggcct gccgctccgc
     2221 ccgttcgagg tcgcgccgcg ccaggtaggc gtaggagagg gccagccgcg cctccggctc
     2281 gtcgtacaag agcagcgtct cctccagccg cgccaccgcc cgcgtatacc gcccctcgtc
     2341 cagcagaccg tatgcctgcc tcacccgggt gcgcccctcc acctccgggt tcttccatga
     2401 ggccacaatc gccagcgcca gcaacgccac cagcaccagg ccaagcaaag tgtcgcgaag
     2461 agagtaatga gtgatgagtg atgaggactg aggaccggat ggctggtgtt gcactactca
     2521 gtcctcatca ctcatcactc attactcgat agacgttgtg gtgctgggcg gctacctttt
     2581 cggtagtgaa gaggcttagc acgcgctctc taccccggct gccaagctgc gcgcgcaggg
     2641 gggcatcgga gaggaggcgg cgcaggtggg aggcgagggc tgcgctgtcg ccttcgggga
     2701 agattagccc ggcgtctccc aaaacggtgg gaatctcgcc ggagtccgaa ccgactacgg
     2761 ggagggcgca ggacatggct tcgacggcca cccgtccgaa ctgctcgcgc cagttggggc
     2821 gggtctcgga gggcagcacg aaaacgtcgg cggcgttcat catgttaggt aactgcgtgt
     2881 tgtgtaccac gcccggcagg cgcacccgcg actccagccc ggcctcacgc gccttctcct
     2941 ccagcttgct cttgagcggc ccgccgcccg ccagcaccag gcacgcgcgc tcctgctcgg
     3001 cggccacctc ctcgaacgcc cgccagagcg acaggaggcc cttctcctcg accagcttgc
     3061 ccgcgtagag cagcacgagg ccatcctggg gtatcccaag ctcgcggcgt tgggcctccc
     3121 ggcgggccgg gtcgggggcg aacgcgcagg cgtcggtggg tagcggcacc acacgcaggg
     3181 agcctcgata gcccttggag cgcaacgtca tagctacagt cgtgccgcaa gccagtatca
     3241 tgtcggtgtt gcggaaggcg taacgctcga acagccgaaa aggcagcggg tactgcttct
     3301 tgatgttctg cgcggcgtaa aggagcatgc gggtgcgcgg caggaagatg cggcgaagca
     3361 gcagcacgag agccgccatg agggtgtggg cctcttcgta gcaatagatg atgtcgggcc
     3421 gcagcctcag caggtggaag agcagggtgg ggaagaagta gatgtagtag aggccggtga
     3481 aagcggtgcg cgccacgatt accctgaagc tcgcgcccgg cagcgtctcg gcgcgcacca
     3541 tctgcccggc cctctccggc caccgcctgg gtagcatgag cgtcacatcc aggtcggggt
     3601 agcccgcaag ctcctgcacc ggcttctggt actcggccac cacagccgcg tgccagatcc
     3661 acaggacggt tagtaatgag gactgaggac tgaggactga gtgttcagtt gtatgtcgtg
     3721 gttctccgtt ggcagggaat tggttcatct cttgtataga ctactacaat cggcgggtct
     3781 tag
//
LOCUS       DASRWZ010000212         4216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1250648, whole genome shotgun sequence.
ACCESSION   DASRWZ010000212 DASRWZ010000000
VERSION     DASRWZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250648"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFH60_03275"
     CDS             complement(<1..881)
                     /locus_tag="VFH60_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312832.1"
                     /translation="MRAGSRPRGRTRKTGPLRIRGFRRGATRSHAAEPLARGLVRLRR
                     VRRGVASHLPNVPLIRQIGRQSASERVEGLSPEAALHASRNFRYGVGNGIAFTVVDSL
                     IAPSLVLAWFINRLGAPNVLVGLLPAILSGGWFLPQMLVASRVQGLKFMMPRYTRVGI
                     MRTVTMGVIALCTVFLAPWPDIMLVVFFVLYTFYAFSAGFSGLPWLEVVGKVIAPRRR
                     GSFFGMRNFWGGLLALLIAAPIGAVLSEQFWGLTFPYNVALLFGVATVCIGVGVWSWS
                     SVHEPPALVTAPPLSVR"
     gene            complement(881..4000)
                     /gene="polA"
                     /locus_tag="VFH60_03280"
     CDS             complement(881..4000)
                     /gene="polA"
                     /locus_tag="VFH60_03280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET6312833.1"
                     /translation="MDTPATRNDDGQASTGAARDAAGDGSRDKLVLVDGHALVYRAYH
                     ALPPDLRTSTGEPTNAVLGFTQMLLDTLRIQAPDYIVVTFDKGRTFRHEASSDYKAQR
                     GPMPDDLHAQMHRVRQIVEALGIPIVELAGFEADDLIGTLSKQAEERGIDTYILTADT
                     DQHQLVSDSVRMIAPGGYKQRFSDARIYDRPAVLERYGFEPTLVPDYKALVGDKTDNI
                     PNVPGIGEKTAAALLVQYGSLEAILEHLDALKPKQQESLREHAEQARQSKVLATIVRD
                     APVELDLAQSRSHGFDKDRVLKLLQELEFRSLVGKVNQVEAVLDNRAASQAGGAAATP
                     GSTATQEPQDGSAPHEQATAEAARSGALQMNMFDEPGVTASAPEPAPAPAGGLDTSKR
                     SSAAAFTDAIESEIAGVPAKVTIVSDVDALRAMVERLRQTGRFTLDVEATSPDDMEAE
                     LVGIALTPGTTDAVVGEAYYVPTGHTQAQGESGLAEAAPGQLSVEQVREVLGPLLLDE
                     SVPKDGHNLKYDIKVLKRAGLPVEGLGHDTMISAFLVGENSIGLKDLAFTKLFVQMVP
                     ITELIGKGRTQTTMNTVPAEVAGKYAAADVAVTERLRHEFVPRLVADGLQKLFDEVEM
                     PLVPVLAEMELTGVAIDITWLQSLSTTMHSKLGELQEQIYAEAKHAFNINSTQQLGVV
                     LFEELQLPGRKRTQKGYSTDREVLDTLRGLQPIVESIIEYRQLIKLKNTYIDALPLLV
                     RRDTGRVHTSFNQTVAATGRLSSSNPNLQNIPIRTEIGRDVRKAFVADNASSHRFFGD
                     EETVLLAADYSQIELRLLADMAGEERLIHAFEAGQDIHAATAAEIMGIPIDQVDPDSR
                     RLAKTVNFGVLYGMGSYGLARDTGLSTKEASEFIELYWSRYPAVRSFMNRTLQEGREQ
                     KYVTTLLGRRRYMPELRSPNAGVRQAGERMAINAPVQGTAADIIKIAMNRLYVEMKRR
                     GLRSKMLLQVHDELLFEAPVSEQQALGDIVCEIMEGAWQLRVPLVAEVKAGRNWGEMQ
                     SVSPTRADK"
BASE COUNT          732 a         1383 c         1310 g          791 t
ORIGIN      
        1 tctcgcacgc ttaagggagg cgctgtgacg agggcgggag gctcgtggac ggaggaccac
       61 gaccacacac ccaccccgat gcataccgtt gccacaccga acagcagggc gacgttgtaa
      121 gggaacgtca gcccccagaa ctgctcggac agcacggctc caatcggcgc ggcgatgagc
      181 agggcgagta gcccgcccca gaagtttcgc atcccgaaga agctgcctct ccgcctcggc
      241 gcgatcacct tgcccactac ttccagccag ggcaggcccg aaaaccccgc cgaaaaggcg
      301 taaaaggtgt acagcacgaa gaacaccaca agcattatgt cgggccacgg cgcgaggaac
      361 accgtgcaca gggctatgac gcccatcgtg acggtgcgca taatgccgac gcgcgtgtac
      421 cggggcatca tgaacttgag gccctgcacg cggctggcca ccaacatttg aggcaggaac
      481 cagcccccgc taagaatagc gggcagcagg ccaaccagca cgttgggagc gcccagcctg
      541 ttgatgaacc aggcgagcac cagggacggg gcgatcagcg agtccaccac agtaaacgct
      601 ataccgttcc ccaccccgta gcgaaagttg cggctggcgt gcagggcagc ttcgggagac
      661 agcccttcca ctcgctccga cgcggattgc ctgccgattt gccggatgag gggcacgttg
      721 ggaagatgcg atgccactcc ccggcgcacc cgcctcaacc tgacaagccc ccgcgccagg
      781 ggttcggctg cgtggctcct tgtcgcgcca cggcggaagc cccttatcct caaagggcct
      841 gtcttgcgcg tgcgtcccct cggccgggag ccggcccgca ttacttgtct gccctggtag
      901 gcgacacgga ctgcatctcg ccccagttcc tgcccgcctt cacctcggcc acgagtggta
      961 cccgaagctg ccaggcccct tccatgattt cgcacacgat gtcacccaac gcctgttgct
     1021 ccgatacggg ggcctcgaat agtagctcgt cgtgcacctg caagagcatc ttgctgcgca
     1081 gcccacggcg tttcatctcc acgtacaggc ggttcatagc gatcttgatg atgtcggcgg
     1141 cggtgccctg cacgggggcg ttgatggcca tacgctcgcc cgcctggcgc acgcccgcgt
     1201 taggggaccg tagctccggc atgtagcggc ggcggcccag cagcgtggtg acgtacttct
     1261 gctcgcgccc ctcctgcaag gtgcggttca tgaagctgcg caccgccggg tagcgcgacc
     1321 agtacagctc gatgaactcg gaggcttcct tggtcgaaag cccggtgtcg cgcgcaaggc
     1381 cataactgcc cataccgtac aacacgccga agttcaccgt ctttgcaagg cgtcgggagt
     1441 cagggtccac ctggtcaatc ggtatgccca tgatctcggc ggcggtggcc gcatggatgt
     1501 cctgccccgc ctcaaaggcg tgtatcagcc gctcctcccc cgccatatca gcgagcaggc
     1561 gcaactcgat ttgtgagtag tcggcggcga gcagcacagt ctcctcgtca ccgaagaagc
     1621 ggtggctgct ggcgttgtcc gcgacgaacg ccttgcgaac gtcgcgccca atctcggtgc
     1681 ggatggggat gttttgcagg ttggggttgc tggacgagag cctgcccgtg gccgccaccg
     1741 tctggttgaa ggacgtgtgc acgcgtcccg tatctcgcct caccagcagg ggcagggcgt
     1801 cgatatacgt gttcttcagt ttgataagct gacggtactc gatgatgctt tccacgatgg
     1861 gttgcagtcc tcgcagcgta tcgagcactt ccctatcggt ggagtagccc ttctgcgtgc
     1921 gcttgcggcc aggtagctgt agctcctcga acagcaccac gcctaactgc tgggtggaat
     1981 tgatgttgaa cgcgtgcttg gcctctgcgt agatctgctc ctgtagctct cccagcttcg
     2041 agtgcatggt ggtggagagc gattgcaacc acgttatgtc aatcgctacg ccggttagct
     2101 ccatctccgc gagcacaggc accaggggca tctcgacttc atcaaataac ttctgcaacc
     2161 cgtcagcaac cagcctgggt acgaactcgt ggcgcagccg ctcggtcacg gccacatcgg
     2221 ccgccgcgta cttgcccgcg acctccgccg gcacggtatt catcgtggtc tgcgtcctgc
     2281 ccttgcctat caactcggtt atgggcacca tctgcacgaa cagcttggta aaggccaggt
     2341 cctttagacc aatcgagttc tcccccacga gaaaagccga gatcatagtg tcatgtccca
     2401 ggccctcgac aggtagtccg gcccgcttga gcaccttgat gtcgtacttg agattgtggc
     2461 cgtccttggg caccgactca tcgagcagga gcggccccag cacctcacgc acctgctcga
     2521 cgcttagctg tccgggcgcg gcttcggcca gacctgactc gccctgggcc tgcgtatgcc
     2581 cggtgggcac gtagtacgcc tcgcccacca cggcatcggt cgtacccgga gtgagggcga
     2641 tacccacgag ttcagcttcc atgtcgtcgg gcgaagtggc ctcgacgtcc agcgtgaacc
     2701 gccccgtctg ccgtagccgc tcgaccattg ccctcagggc gtccacgtcc gagacaatcg
     2761 tcaccttcgc gggaacgccg gcgatctcgc tctcgatagc atctgtaaac gcggcggcgc
     2821 tggaccgctt cgacgtgtcg aggccaccgg cgggggcggg ggcaggttcg ggcgcactgg
     2881 cagtgacacc cggctcgtcg aacatgttca tctgcaaggc accggagcgc gcggcttcgg
     2941 cggttgcctg ctcgtgggga gcggagccgt cctgcggctc ttgcgtggcg gtgctccccg
     3001 gtgtggcggc agccccgcca gcctgggagg ccgccctgtt atccaaaacg gcctctacct
     3061 ggttcacctt gcccaccagg ctgcgaaact ccaactcctg gaggagcttc aacacgcggt
     3121 ccttgtcgaa gccatggctg cggctctgcg cgaggtcaag ctccaccggc gcgtcccgca
     3181 cgatggtcgc cagcaccttg ctctgtcgcg cctgttcggc atgctcgcgc agcgattcct
     3241 gctgcttcgg cttaagcgcg tcaaggtgct cgagtatcgc ctcaaggctg ccgtactgca
     3301 ccagcagggc cgccgcggtc ttttctccga tgccggggac gttcggtatg ttgtcggtct
     3361 tgtcgcccac gagcgccttg tagtcgggca cgagggtagg ctcgaacccg tagcgttcga
     3421 gcacggcggg cctgtcgtaa atgcgggcat cggagaagcg ctgcttgtag ccgcctgggg
     3481 cgatcatacg caccgagtcg gacacaagct ggtgctggtc cgtgtcggct gtgaggatat
     3541 aggtgtcgat gccgcgctct tcggcctgct tggagagggt gcctatcagg tcgtcggcct
     3601 caaaacccgc cagttcgaca atcgggatgc cgagggcctc cactatctgg cgcacccggt
     3661 gcatctgggc gtgcaggtcg tccggcatgg ggccgcgctg ggctttgtag tcggacgaag
     3721 cctcatgccg gaaggtgcgc cccttgtcga aagtgactac tatgtaatcg ggggcctgga
     3781 ttcggagggt gtcgagcagc atctgggtga agcccagcac cgcgttggtc ggctccccgg
     3841 tggaggttct caggtcgggc ggcagggcat ggtaggcgcg gtacacgagc gcatgcccgt
     3901 ccaccagcac cagcttgtcc ctggagccgt ctccggcagc gtccctcgcg gccccggtcg
     3961 aagcctgtcc atcgtcgttt ctcgtggctg gcgtgtccaa gattcagcta cactccttct
     4021 agtaatgcac cctctattat accccctgcc gggtactctc gacacgcttt gcccagcaac
     4081 tgcgcgaaga gacaaagtcc tcacctgacc gccaaattcc agtgcgcgcg aggccgcggc
     4141 aggtgggtgc gccctgccac taaagtggcg ggcttacaag aggcaaagtc ccctacgggg
     4201 actgaggaac atcatg
//
LOCUS       DASRWZ010000213         4800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1044867, whole genome shotgun sequence.
ACCESSION   DASRWZ010000213 DASRWZ010000000
VERSION     DASRWZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044867"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="VFH60_03285"
     CDS             complement(<1..1683)
                     /locus_tag="VFH60_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312834.1"
                     /translation="MVGLLRWWGVWLVLLLVVAGSVAAYQRPATYVVDVGSRQDQAYT
                     RNFHARLEEPGRTFRWSDVYGYVTFPGVGGARPFTVTVALDPARPADVEIFVNGDRFF
                     AGDVGPGWREFAFPVDESHPAALASRDTVVEFRAPEYRAAGEAGEAKGLKVDRVVLEQ
                     APEGGFIWPSLATVALTVLSTLLVYLLVGRALAGISHSEQGRGRAVVLALVAGTALCL
                     LLAASRTAASAAVPHVAATLASMLVILVVAERLAAGLFAGIGGRGARLLALAVAVAFG
                     VRFGGMALPQSVIIDMPYHMKWLRTLLAGDWQALYFPGGLSEVPPEWGMSLLIPKSPL
                     FYLAAAPLNVIPFDLETLVKWLICSLDASLVVLASWFAMRVGASRRAALAAACLYAVM
                     PLAFRAFSYGILPTIFAQWLAAGLFAFVLAFSVKGWRPATLLVGTLLATLTLLAFPTV
                     AVFVTLVVAFVPLGWRVAGRKEATRSFNWQPYVMLSVAWLLAIVVYYGLYIEPVVASA
                     QALLTPAPGGGTTVRWPGGVAELVVWTADYLASTLPVVLAALGALILWARRRA"
     gene            complement(1769..3835)
                     /locus_tag="VFH60_03290"
     CDS             complement(1769..3835)
                     /locus_tag="VFH60_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312835.1"
                     /translation="MIRHSNLLRRLLNPGVFAGVLATLALVVLAYQVRPTYAIGVGTP
                     PDTSIVRGFNTPEQMQGEVPQAFRWTTEESFITLRDVGRQDFSVILTVSGFRPEGQEP
                     ARLKVDVGGRTVLDVEPAPQLTNYSFEVSRDDVSDGTLVMKLSSNTFVPPGDPNPRPL
                     GVVVTGALVEPGTHPDRFIEPPWRVVVGLVSAAAMIGVLLALLGWGVGVVAGGSALLG
                     LLAGWLLVSNRLWLTSGRWYESWPQALLAGAAFAGLVAGAGWLLQRLTGARWPALDRR
                     ILLSVMLLAFVVRFAGQLHPLIHIIDLVFHSHRFDTVQAGQLLFTIRSDEWGGHETFY
                     LPTPYVFMLPLQWLLNDQLLVIRLSTVALGTLGAGLLYLTAVASTGDRRAALLASALY
                     VVVPIAVLPYSWGITSNLFGEFFALCAMTLLVTCYRYIRPTRPAFWALLLLLLPALLS
                     HPGVVQLTGLAVGLTGLLWTLFGRRSQPGIGRAVPPGVWVLSALLLGVGLAYMLYYGY
                     FAADMLGTLQQIRAERAAQAQPGAVHLRVGGSVSDRSLGLIVRYAETRREWLVGGLLG
                     FWNEAQAYYRVWPIGAALLGFVSVWPSRRLWGLWAVKRRVLVLTALGWGGAVFAFALV
                     GWTMNLYVRYALFALPLVCLGSGVVLSKFWRRGWAGSLLVCLVLAFFAFEALNLWQYR
                     ITYAFK"
     gene            complement(3881..>4800)
                     /locus_tag="VFH60_03295"
     CDS             complement(3881..>4800)
                     /locus_tag="VFH60_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312836.1"
                     /translation="VFGLGGGGILAALLYLTLPISVLPYSWGITPNVFGEFFLLCSLA
                     VALGSVGKPAPARPAFWVLVALLTITLLSHPGVVALSGLAFALLGALWVARRGPLRRA
                     GVWLLVSLALAAGISYAVYYHHFTGQMLDSLARIGAERAQASEGAIFSRVVGGSVEDV
                     SLGLINREVHSRREWVLGGLEGVWRESVAYYRGWPVLAALLGLALVWPARRLLRARGG
                     SVRLRFALAGSVWLGVVALFAVVGWATGIYVRYMLSALPIVALGAGLLLSAFWRRGRL
                     GRWLSVLVLVFFAFEAVLLWHYRISYQFK"
BASE COUNT         1054 a         1780 c         1373 g          593 t
ORIGIN      
        1 agcacgcctg cgcgcccata ggatgagcgc tccgagcgcg gcaagcacga cgggaagggt
       61 gctggcgagg tagtcggccg tccacaccac gagttcggcc acgccacccg gccagcgcac
      121 ggtggtgcct cctccgggtg ccggggtgag gagggcctgc gccgaagcca ccacaggctc
      181 gatgtacagg ccatagtaca ccacgattgc aagtagccac gccaccgaca acatcacgta
      241 gggctgccag ttgaacgacc gcgttgcctc tttgcggccc gccacccgcc agccgagagg
      301 cacgaaagcc accaccagcg ttacaaacac ggccactgta ggaaacgcca gcagggtgag
      361 cgtggcgaga agcgtcccca ccagcagggt agcaggacgc caacccttca ccgaaaaggc
      421 cagcacgaac gcaaacagac ccgccgcaag ccactgggca aaaatagtcg gcaagatgcc
      481 gtaggagaaa gcgcggaagg ccaatggcat gaccgcatac aggcaagcag cagccagcgc
      541 ggccctccta gacgcgccca cacgcatcgc aaaccaggac gcaagcacca ccaagcttgc
      601 atcgagggag caaatgagcc acttgactaa tgtctcaagg tcaaatggta tgacattcaa
      661 cggtgccgcg gcaaggtaga acaggggcga cttcggtatc agcaaggaca tgccccactc
      721 cggcggtact tcgctcaggc cgcccggaaa gtagagggct tgccagtcac ccgcgagcaa
      781 ggtacgtagc catttcatat ggtagggcat gtcgataatc acagattgcg gcagcgccat
      841 gcccccaaac cgcacgccaa acgccaccgc gaccgccaac gccagcagcc tggcccctcg
      901 tcctcctatc cctgcgaaaa gccccgctgc cagccgctcg gcaacaacca ggatcaccag
      961 catgctggcc agggtcgccg cgacgtgcgg caccgccgca gacgctgccg tacggctcgc
     1021 cgcaagcagc agacatagcg cggtcccagc caccagggcc agcaccaccg cccgccccct
     1081 cccctgctca gagtggctga tgcctgccag cgcgcgtccc accagcagat agacgagcag
     1141 ggtgctcaac acggtgagcg ccaccgtcgc caacgaaggc cagatgaagc cgccctccgg
     1201 tgcctgctcg agcacaaccc tgtcaacctt cagtccctta gcctcgcctg cttctccggc
     1261 ggccctgtac tcgggcgcgc gaaactccac aaccgtgtcg cgggacgcca gcgcggccgg
     1321 gtggctctcg tcaaccggga aagcaaactc ccgccagccc ggtcccacgt caccggcaaa
     1381 gaacctgtcc ccgttgacga atatctccac gtcggcaggt cgtgccgggt cgagtgcgac
     1441 ggtgactgtg aaggggcgcg cgccgcccac tcccggaaag gtcacgtagc catatacgtc
     1501 cgaccacctg aatgtgcggc ccggctcctc gaggcgtgcg tggaagttgc gcgtgtaagc
     1561 ctggtcctgc cgcgagccga catccacaac ataggtagca ggacgctggt aggccgctac
     1621 agagcctgcc acaaccagca gaagcaccag ccacacaccc caccaccgca gcagccccac
     1681 cacgccagcc gcctcatccc gcagccaagt gacaagggtt tgcaaacgcg gtagcactct
     1741 acccggtgtt cgtgccagag cttgcaggct acttgaaggc ataggtgatc ctgtactgcc
     1801 acaggttgag tgcctcgaac gcgaagaaag caagcaccag gcagaccagc agcgaccccg
     1861 cccagccccg ccgccagaac ttcgagagta ccaccccgct gcccaggcac accagcggca
     1921 gggcgaacag cgcgtaccgc acgtagaggt tcatcgtcca ccccaccagc gcgaacgcga
     1981 acacggcccc accccaaccg agggccgtca gcaccagcac ccgccgcttc actgcccata
     2041 agccccacaa gcgtcgggag ggccacacgg acacgaaccc cagcagcgcc gcgccaatgg
     2101 gccacaccct gtagtacgcc tgcgcctcgt tccagaagcc cagcaggccg cccaccagcc
     2161 actcacggcg ggtctcggcg tagcgtacta ttagccccag gctccggtcc gagaccgacc
     2221 ctcccacccg caggtgcacc gctcccggct gggcctgggc cgcacgctcg gctcgaatct
     2281 gttggagggt gcccagcata tccgccgcga agtagccgta gtacagcatg taggccagcc
     2341 cgacgcccaa cagcagcgcc gagagcaccc acacccccgg cgggaccgct cgccctattc
     2401 caggctgaga cctccggcca aacagggtcc acagcagccc ggtcaggccc accgccaggc
     2461 ccgtaagctg taccacgccc gggtggctga gtagcgcggg cagcagcaat aacaggagcg
     2521 cccaaaaggc cggtcgcgtt ggccgtatgt agcggtaaca ggtcacgagg agcgtcatag
     2581 cgcacagggc gaagaactct ccgaacaggt tcgatgttat gccccacgag tacggtagca
     2641 cggcgatggg cacaaccacg taaagcgcgg aggccagcaa agccgcccgc cggtcgccgg
     2701 tggacgccac agcggtgaga tacagcagcc ccgcccccag cgtgcccagc gccaccgtag
     2761 aaagccttat cacaagcaac tggtcgttca gaagccactg caacggcagc atgaagacgt
     2821 agggcgtcgg caggtagaaa gtctcgtgcc cgccccattc gtccgacctg atggtgaaca
     2881 gaagctgccc ggcctgcacc gtgtcgaacc tgtggctgtg gaagaccagg tcgatgatgt
     2941 ggatgagcgg gtgtagctgc cccgcgaacc gcaccacgaa cgccagcagc atcacgctca
     3001 gcaggatgcg acggtcgagg gcaggccaac gcgcccccgt gagccgctgc aagagccaac
     3061 ccgcccccgc caccagcccc gcgaaggccg ccccggcgag cagcgcctgg ggccagctct
     3121 cataccatcg ccccgacgtg agccagagcc ggttcgacac cagcagccac cccgccagca
     3181 gccccaggag ggcagacccc ccggccacca cgcccactcc ccagccaagc agcgccagga
     3241 ggacgcctat catcgcggct gcgcttacca accctaccac cacacgccag ggcggctcga
     3301 tgaagcggtc cgggtgggtg ccaggctcga ccagagcccc cgtgacgacc acacccagag
     3361 ggcgggggtt agggtcgccg ggaggcacaa aggtgttgct cgacagcttc atcaccagcg
     3421 tgccatcgct tacgtcgtcc ctcgacacct caaacgagta gttggtcagt tgtggcgcag
     3481 gctctacgtc cagtacggtg cgccctccaa cgtccacctt gagcctggcg ggttcctgcc
     3541 cctccgggcg aaagccgctc acggtgagga tcacgctgaa gtcctgcctg cccacgtctc
     3601 gaagcgtgat gaacgactcc tcggtggtcc acctgaacgc ctgcgggacc tctccctgca
     3661 tctgctcagg cgtgttgaag ccccgcacga ttgaggtgtc gggcggcgtg cctacgccaa
     3721 tcgcgtaggt cggcctaacc tggtaggcga gcaccaccag cgccagcgtc gccagcaccc
     3781 cggcaaacac gcccggattc agcaaccgcc tgagcagatt ggagtgtcta atcaccctca
     3841 ccgtcctaaa tccgcaatcc taattcccaa gtcccaaatt tcacttgaac tggtagctga
     3901 ttctataatg ccacagcaac accgcctcaa aggcgaaaaa caccagcacg agcaccgata
     3961 gccatctccc caagcggccc ctgcgccaga acgccgagag caacagccct gcgcccaggg
     4021 cgacgatagg cagggccgac agcatgtagc gcacgtatat gccggtggcc caacccacca
     4081 ccgcgaacag cgccacgaca cccagccata cgctgcccgc gagcgcaaat cgcaagcgca
     4141 ccgaaccgcc acgagcgcgc aacaacctcc tggcgggcca cactagcgcc agccccagca
     4201 gggccgcgag caccggccag ccgcgatagt atgccacgct ctcccgccac acaccttcca
     4261 gtccaccaag cacccactcc cgccggctgt ggacctcgcg gttgatgagg cctaggctca
     4321 cgtcctccac cgacccgccc acgacgcggc tgaagatcgc cccctcgctc gcctgcgccc
     4381 tctccgcccc aatccgcgcc aggctgtcca gcatctgccc ggtgaagtgg tggtagtaga
     4441 ccgcgtacga aatgcccgcg gcaagcgcca gcgacaccag gagccacacc cctgcccgcc
     4501 gcagaggccc cctgcgagcc acccacagcg ccccaagcaa tgcaaaagcc aacccgctaa
     4561 gcgccaccac gcccgggtgg ctgagcaatg ttatcgtcag cagtgcaacg agcacccaga
     4621 aggccggtcg cgcgggggcc ggctttccga cgctacccaa cgccacggcc agcgagcaca
     4681 acaggaagaa ctcgccgaat acgttcggcg tgatacccca ggagtaaggc agcaccgaga
     4741 tcggcagggt cagatagagc agagccgcaa gaatcccccc cccccccaac ccaaaaacaa
//
LOCUS       DASRWZ010000214         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_702227, whole genome shotgun sequence.
ACCESSION   DASRWZ010000214 DASRWZ010000000
VERSION     DASRWZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702227"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFH60_03300"
     CDS             <1..210
                     /locus_tag="VFH60_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312837.1"
                     /translation="LVLPDDNVLDLHIDTDDESFLTVDGQTHVPLRDGDTVRVTISPD
                     PCLFARVQDKAYFSATLVNRLRRAE"
     gene            complement(382..2706)
                     /locus_tag="VFH60_03305"
     CDS             complement(382..2706)
                     /locus_tag="VFH60_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET6312838.1"
                     /translation="MHRSSHPGIQRWSARKVASTLLALCLPVILATFFSGGFLRSGSG
                     TASQVFDAPSAHAAAPHAPQANASVSITDNGLNPSTLNVTPGTTVTWTNNSSEERRID
                     ETSNHIFRSDDLEPGDTFSYNFSTPGTFYYEEDRDDFQGTIIVSGPTPTPSGGLGTTV
                     DVSMESGDNFNPTTVYINTGTTVRWTNNDGEEHSSSSTNATGDNAWQSPPLNEGESWS
                     KTFLNPGTFTYLCTFHDDEMFGTVVVSGAAISPTPGPATSTPASGTSTVNIQGYQYQP
                     KDHVVALGSTVRWTNLDADSHSATSSTGAFDSGGLGQNGTYEKTFTTAGRYDYFCMYH
                     PGMTGSITVQTSDGSVPTAGPSNTPTMTRTPQPTNTPGGAPVTEASIVDYSYNPTTIN
                     VQPGTTVRWTNNGQAPHTVTSDPAGQFASGLMSSGAAWQFTFNTVGTYAFFCDFHSNM
                     RGTVNVLAPGSTPIPQPSNTPRATSTPMSQPSPGTVRVAIENFVYEPVTATVNLGDKV
                     IWTNFDTAPHSVTSNGEHAGLFDSGEFGLNGTYERVFDTPGTFKYYCTVHGNYMTGYV
                     VVRNSGGVFADVAPSNTFYPYIVCLADRSVLGGYPDGTFRPGNQVTRGQLAKIIANSA
                     GFNETPSGQSFADVAPGSTFYDYVERIASRGIIGGYPCGGPGEPCTAGNKPYFRPGDN
                     ASRGQIAKIVAGAANLTGTPSRQTFADVPTNHTFYSFVEQLSANGAMSGYACGGTGEA
                     CDSQNRPYFRPNVKTTRGQIAKIVSMSFFPECAN"
BASE COUNT          541 a          819 c         1059 g          620 t
ORIGIN      
        1 ctggtgctcc ccgacgataa cgtgctggac ctgcacattg acaccgacga cgagtcgttc
       61 ctcacggtgg acggccagac gcacgtcccc ctccgcgacg gcgacacggt gcgcgtcacc
      121 atctcccccg acccctgcct cttcgcccgc gtgcaggaca aagcctactt ctcggccacg
      181 ctagtgaaca ggttgcggag agcggaataa gagagacgaa aacgcaaaga cgcaaagagc
      241 gcaaaggacg cgcaaagtct cggattgttt agagggtgca aaaaggacca gggtacatgc
      301 tgttgtaccc tggtcctttt gcttaaaacg cctatttaat tttggcgtta tcttggcgtc
      361 ttggcggttc cgtctcctgc cctagttcgc gcactccggg aagaagctca tcgagactat
      421 cttggctatc tggccgcggg tggtcttgac gttggggcgg aagtaggggc gattctggct
      481 gtcgcaggct tcgccagtgc cgccgcaagc gtagccgctc atcgcgccgt tggccgacag
      541 ttgctccacg aagctgtaga aggtgtggtt ggtgggcacg tccgcgaagg tctggcggga
      601 gggggtgccg gtcaggttgg ccgcgcccgc cacgatcttg gctatctggc cgcgagaggc
      661 gttgtcgccg gggcggaagt aaggcttgtt gcccgcagtg catggctcgc cggggccgcc
      721 gcacgggtag ccgccgatga tgccgcgcga ggcaatgcgc tcgacgtagt cgtagaaggt
      781 gctgccgggg gccacgtccg cgaaggactg cccggagggc gtctcgttga agcccgccga
      841 gttggcgatg atcttggcga gctggcctcg cgtcacctgg ttgccggggc ggaacgtacc
      901 gtccgggtag ccgcccagca ccgagcggtc ggccaggcag acgatgtagg ggtagaaggt
      961 gttgctgggg gccacgtccg cgaagacgcc gccggagttc cgcaccacca cgtagccggt
     1021 catatagttg ccgtgcacgg tgcagtaata cttgaacgtg ccgggcgtat cgaacacgcg
     1081 ctcgtaggtg ccgttcaggc cgaactcacc gctgtcgaac aggcccgcgt gctcgccgtt
     1141 gctggtgacg ctgtgcgggg cggtgtcgaa gttggtccag atcaccttgt cgcccaggtt
     1201 caccgtagcg gttacgggct catagacgaa gttctcaatc gcaacgcgta cggtgcccgg
     1261 agatggctgg ctcatcgggg tggaagtggc cctcggcgta ttgctgggct gcggtatggg
     1321 cgtgctgcct ggggcgagca cgttgacagt gcccctcatg ttgctgtgga agtcgcaaaa
     1381 gaaagcgtag gtgccaaccg tattgaaggt gaactgccac gccgccccac tggacatgag
     1441 gccgctggcg aattggcccg ccgggtcgct ggtgacggta tgcggagcct ggccgttgtt
     1501 ggtccagcga acggtggtgc cgggctgtac gttgattgtc gtggggttgt agctgtagtc
     1561 aactatggac gcttcggtca caggggcacc accgggcgtg ttcgtgggct ggggtgtgcg
     1621 cgtcatggtc ggcgtgttgc tggggcctgc ggtgggtacc gagccgtcgc tggtttgcac
     1681 ggtgatgcta cccgtcatac cggggtggta catgcagaag tagtcgtacc tgcccgccgt
     1741 ggtgaaggtc ttctcgtaag tgccgttctg gccgaggccg ccgctatcga acgcgcccgt
     1801 gctgctggtg gcgctatggc tgtccgcgtc taggttggtc cagcgcacgg tagagcctag
     1861 cgcgacaacg tggtccttgg gttggtactg gtagccctgg atgttcacgg tggaagtacc
     1921 ggaggccggg gtactggtgg cggggccggg ggtaggcgag atggcggcac ccgataccac
     1981 gaccgtgccg aacatctcgt catcgtggaa ggtgcacagg taggtgaagg tgccggggtt
     2041 gaggaacgtc ttagaccacg actcgccctc gttgagcggg ggactctgcc aggcgttgtc
     2101 gccggtggcg ttggtgctcg aggagctatg ctcctcgccg tcgttgttgg tccagcgcac
     2161 ggtggtgccg gtgttgatat agacggtggt ggggttgaag ttgtcgccgc tctccatcga
     2221 gacgtccacg gtggtgccga ggccaccgga gggggtgggt gtcggaccgg acactataat
     2281 ggtgccctgg aagtcatcgc ggtcctcttc gtagtaaaag gtgcccggag tggagaagtt
     2341 gtaagagaag gtatcgcccg gctccaggtc gtcgctgcgg aagatgtgat tgctcgtctc
     2401 atcgatgcgg cgctcttccg agctgttgtt agtccacgtc accgtggtac cgggggtcac
     2461 gtttagagtc gagggattga ggccgttgtc ggtgatggat acgctagcgt tagcctgcgg
     2521 ggcgtgcggg gctgccgcgt gcgccgatgg ggcgtcaaac acctgggagg cagtgcctga
     2581 gcctgaacgt aaaaagccac ctgagaagaa cgttgccagg atgacgggca gacaaagtgc
     2641 gaggagcgta gacgcaacct tgcgggcgct ccacctctgt atacctgggt gcgatgatcg
     2701 atgcattcct ctttcactcc ctttttgtgc tatgcacgct aaccgagtgc gacctaatat
     2761 caccaagtcc tggataaaat acggacaagg gttacgatat aaaattcgta ccctgggagg
     2821 tggggtattc cggctcgtaa catataagtt taccaaaatc gaggggccaa ccgcaaacat
     2881 acagggagcg ccaaggggag agttttcgtg cgtggggcgc tatccatgtc gaaccggcag
     2941 gaccgaagtg gcccctgtca tcctgagcgt agcgaaggat ctaaagaagc tggtactgga
     3001 tgctgtcgaa catcacgaat catggtgcaa acgttcaac
//
LOCUS       DASRWZ010000215         5159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_771094, whole genome shotgun sequence.
ACCESSION   DASRWZ010000215 DASRWZ010000000
VERSION     DASRWZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771094"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            475..2115
                     /locus_tag="VFH60_03310"
     CDS             475..2115
                     /locus_tag="VFH60_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET6312839.1"
                     /translation="MGTDLADDVIEGIPANSEPPEGKAVALSRPLIITGLIMATALSA
                     LDVTIISTALPTIVGVLGGLEFYPLVVAAFLLTATTTVPVYGKLSDMFGRKPVFLIGA
                     GVFILGSALCGLAWDMPSLIAFRAMQGLGAGSIVPVSLTLIGDLFDVEERARIQGVFG
                     SVWGVSSIIGPLVGGAIVQFFDWRWAFFINVPVGLLSMLLIFLYLREPAIHTRQRIDF
                     GGAITLTLGMGLVLVALQSGSRGGWLSPITLVFWAGAVGLLALFVAYERRAPAPILSF
                     SLLSRPVIAIPCLVGLLAGVVLIGYAAYVPLLVQGAWGGSPIEAGLVVAPMSIGWPVA
                     SAMSGRLIKRVGYFPLVATGATAIAAGTLLLLGVGLVSDISLKTAITLVASGITGLGF
                     GASTTTMLIALQVSVPWSERGVATASAQFFRNMGQAIGATVLGTVLTLLLAPMLASER
                     VQQAVAAMPPGALKAGADPALGPANALFDLDVRPTLEPDVVGALADALSNSLWWMFVA
                     MLLISVVGLLVATRFPRVVTPAGTGATATPAPRPISME"
     gene            2229..3863
                     /gene="treS"
                     /locus_tag="VFH60_03315"
     CDS             2229..3863
                     /gene="treS"
                     /locus_tag="VFH60_03315"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HET6312840.1"
                     /translation="MSDSLWYKDAIIYELHVKAFYDSNGDGIGDFNGLTEKLDYLQDL
                     GIDTIWLLPFFKSPLRDDGYDIADYYQVHPNYGTVADFKRFLKEAHRRNLRVLADLVM
                     NHTSDQNAWFQEARASTDPKKRNWYVWSDTIHKYEQARIIFTDTERSNWTWDETAKAY
                     FWHRFFSHQPDLNFDNPAVRREMLRVIKFWMDTGLDGFRADAVPYLFEREGTNCENLP
                     ETHEYLKQVRKFMDEHYEGKVLLAEANQWPADLLPYFGNDDEFQMCFNFPLMPRIFMS
                     LRREERWPIIEIISQLPKIPERCQWGIFLRNHDELTLEMVTDEERDYMYYEYAKDPRM
                     KINIGIRRRLAPLLDNGRRQIELLNNLLLTLPGTPVLYYGDELGMGDNIFLGDRDGVR
                     TPMQWTPDRNAGFSRADPARLYSPVINDPVYGYQSINVEAQMRTQTSLLRWMRRMIHV
                     RKKYPVFGRGDIEFLNPANPKVLAFIRHHEDVDVLCISNLSRFAQPVELDLSQYNGRR
                     PIELIGDTPFPKIGELPYFLTLGPHGFFWFRLLREG"
     gene            4152..5036
                     /locus_tag="VFH60_03320"
     CDS             4152..5036
                     /locus_tag="VFH60_03320"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET6312841.1"
                     /translation="MATGVAQAIIDRQKWLDDISDKVQPLINNTFSSGGEVGKVAKDF
                     LNGVWLGHPLHPVITDVPVGGWTITQLLDVISMLRGGDDGLDAAADIALGAGIVAAVG
                     AAVTGLADWSDVGGSHRRMGMAHALLNVGGLTLNVASLALRVGGKKNRGVARSLSAGG
                     YVLNAAAAYVAGELVYNLGQAVNRDAWVDGPDKFTDVASLEDLREGEMVKVDVDGRPV
                     VLLQHDDGIHAFEGTCPHYGCGLWEGILEDHTVTCQCHGSQFDVRDGTLIHGPATAPV
                     PSYDVRRRMGRVQIRMRK"
BASE COUNT          917 a         1691 c         1546 g         1005 t
ORIGIN      
        1 ccatactctc gcccttgctt gcctacgcct gggggaggag cactccgagg ctcgtggtgt
       61 agaccgcctg cccaccgctg tgaccgaccc actcgcagcg gcgctcgttc ggtctctcga
      121 tgatgctgat ttgcgccgag ccttgtccgt ggcgacaacg tcttttatca ccgaacttga
      181 agaacaggac tccgcccttt gcgtacgtct caggccttta ctccaagagt tcggcgctcc
      241 gctgagtggt tcgatataag cgtcacgttg aggattccgc caggtctata aagcttcgta
      301 gtatagacct ggtccttctg ctttcgaact gtttttgagt tgagttctag tgaagatagt
      361 ttcggaggga ctttgctcct tttccgtccg ccggctctaa gcagggctac acaggtcggc
      421 acgcgacgtg caatctaatc tacctcgcaa cttcacaaac acaggggcgt aatcttgggc
      481 acagacctgg cagacgacgt tatagagggc atccccgcca atagcgaacc gccggagggc
      541 aaggcggtag ccctcagcag gccgctcatc atcaccggcc tcatcatggc gaccgccctc
      601 tccgcgcttg acgtgactat catctccacc gcactaccca ccattgtggg cgtgctgggc
      661 gggcttgagt tctacccgct ggtggtcgcc gcgttcctgc tgacagccac gaccaccgtg
      721 ccggtctacg gcaagctgtc tgacatgttc ggccgcaagc cggtcttcct catcggcgcg
      781 ggcgtcttca tcctggggtc tgccctctgc ggcctggcct gggacatgcc ctcgctgata
      841 gccttccgtg ccatgcaggg gctgggggca ggctccatcg tgccggtgtc cctcaccctg
      901 attggcgacc tgttcgacgt ggaggagcgg gccaggatac agggcgtgtt cggctctgtc
      961 tggggcgtgt cgagcatcat cggccccctg gtgggagggg ccatcgtgca gttcttcgac
     1021 tggcggtggg ccttcttcat caacgtgccg gtgggcctac tgtcgatgct gctcatcttc
     1081 ctctacctgc gcgagcctgc catccacacc cggcagcgca tcgattttgg gggcgcaatc
     1141 accctcacgc tgggcatggg gctggtgctg gtggccttgc aatcgggttc caggggcggc
     1201 tggctgtcac ccatcaccct ggtcttctgg gcgggagcgg tggggctact ggccctgttc
     1261 gtcgcctacg agcggcgcgc cccggccccc attttgtcgt tcagcctgct ctcccgcccg
     1321 gtaatcgcca tcccgtgcct ggtggggtta ctggcgggcg tggtgctcat cggctacgcg
     1381 gcctacgtgc ctctactggt gcagggtgcg tggggcggct ctcccataga ggccgggctg
     1441 gtggtggccc ccatgtcgat aggctggccc gtggcttccg cgatgagcgg ccgcctcatc
     1501 aagcgggtgg ggtacttccc gctggtggcg accggcgcga cggccatcgc tgcgggcacg
     1561 ttgctcctgc tgggcgtggg gctggtgagt gacatatccc tcaagacggc aatcacgctg
     1621 gtggcctcgg gcatcacggg cctcggtttc ggcgcaagca ccaccaccat gctgatcgcg
     1681 ctacaggtgt cggtgccctg gtcggagcgt ggagtcgcca cggcctcggc gcagttcttc
     1741 cgcaacatgg ggcaggccat aggggccacc gtgctcggca cggtgcttac tctgctgcta
     1801 gccccgatgc tggcttccga gcgagtgcag caggctgtcg cggccatgcc ccccggcgcg
     1861 ctgaaggccg gcgcagaccc cgctctcggt cccgccaacg ccctgttcga cctcgatgtg
     1921 cgccccactt tagagcccga tgtcgtgggt gccctggccg acgcgctctc caacagcctc
     1981 tggtggatgt tcgtggcgat gctgctaata tccgtcgtgg gactgctggt agccacccgc
     2041 ttccccaggg tagtcacccc agctggcaca ggcgcgaccg ccacgcccgc cccgcgcccg
     2101 atttccatgg aatgaaggtt gctaggacaa cgaatttgga aattggagtt tcgaatttcg
     2161 aatttagata agtatccagc aaattcgaaa tccgcaatcc caaattccaa attccacttg
     2221 gaggaactat gagcgatagc ttgtggtaca aagacgcgat tatctacgag ctgcacgtca
     2281 aggcgttcta cgacagcaac ggcgacggca ttggcgactt caacggcctc accgagaagc
     2341 tcgactacct gcaagacctc ggcatcgaca ccatatggct gctgcccttc ttcaagtcgc
     2401 ccctgcggga tgacggctac gacatcgccg actactacca ggtgcacccc aactacggca
     2461 cggtggccga cttcaagcgg ttcctcaagg aggcccaccg ccgcaacctt cgcgttctcg
     2521 ccgacctcgt gatgaaccac acctccgacc agaacgcctg gttccaggag gcccgcgcct
     2581 ccaccgaccc caagaagcgc aactggtacg tgtggagcga cacgatacac aagtacgagc
     2641 aggcccgcat catcttcacc gatacggagc gctccaactg gacctgggac gagacggcca
     2701 aagcctactt ctggcatcgc ttcttcagcc accagcccga cctcaacttc gacaacccgg
     2761 ccgtgcgccg cgaaatgctg cgcgtcatca agttctggat ggatacgggc ctcgacggct
     2821 ttcgcgccga cgccgtgccc tatctgttcg agcgcgaggg caccaactgc gagaacctgc
     2881 ccgagacgca tgagtacctc aagcaggtgc gcaagttcat ggacgagcac tacgagggca
     2941 aggtgctgct cgcggaggcc aaccagtggc ccgctgacct gctgccttac ttcggcaacg
     3001 acgacgaatt ccagatgtgc ttcaacttcc ccctgatgcc ccgcatcttt atgtcgttgc
     3061 gccgcgagga gcgctggccc attatcgaga tcatcagcca gttgcccaag ataccggagc
     3121 gctgccagtg gggcatcttc ctgcgcaacc acgacgagct gacgctggaa atggtcaccg
     3181 acgaagagcg ggactacatg tactacgagt acgccaaaga cccgcgcatg aagatcaaca
     3241 tcggcatccg ccgccgcctg gccccgctcc tggacaacgg ccgccgccag attgagctgc
     3301 tcaacaacct gctgctcacc ctgcccggta cccccgtgct ctattatggc gacgagcttg
     3361 gcatgggaga caatatcttc ctgggcgacc gcgacggcgt gcgcaccccc atgcagtgga
     3421 cgcccgaccg caacgcgggc ttctccaggg ccgaccccgc ccgcctctac agcccggtca
     3481 tcaacgaccc ggtgtatggc taccagtcga tcaacgtaga ggcccagatg cgcacccaga
     3541 cgagcctcct gcgctggatg cggcgcatga tccacgtgcg caagaagtac cccgtcttcg
     3601 gcaggggcga catcgagttc ctcaaccccg ccaaccccaa ggtgctggcc ttcatacgcc
     3661 accacgagga cgtggacgtg ctgtgcatct ccaacctctc ccgcttcgcc cagccggtag
     3721 agttggacct gtcgcagtac aacggccgcc gcccgataga acttatcggt gacacgccgt
     3781 tcccaaagat aggcgagcta ccctacttcc tcacgctcgg cccccacggt ttcttctggt
     3841 tccgcctcct acgcgagggc tagattgtga tgggttaaga gacggaaccg ccaagacgcc
     3901 aaggacgcca aggaccacgc caagattagg tagggttctt agcaaaagga ccagggtact
     3961 tcatcgtgta ctctggtcct ctttgtatca tctagaccat cttaatcttg gcgcgctctt
     4021 ggcgtccttg gcgtcttggc ggttccgtct ctcaagtcac tatcattcca gtgctgaact
     4081 cccggcactc tactggcacg ttttgtgcac cccatacgcg ctgactgaat accctcatat
     4141 ggaggcacta gatggctacg ggtgttgctc aggcaatcat cgacaggcaa aaatggctgg
     4201 acgacatatc cgacaaggtg cagccgctca tcaacaacac cttcagcagc ggcggcgagg
     4261 tgggcaaggt cgccaaggac ttcctcaacg gcgtctggct cggccacccc cttcaccccg
     4321 tgattaccga cgtgcccgtg ggcggctgga cgattaccca gctactagac gtgattagca
     4381 tgcttcgcgg cggcgatgac ggcctggacg cggctgctga catcgcgctg ggtgccggta
     4441 tagtagcggc ggtgggtgcg gcggtcaccg gccttgccga ctggagcgac gtgggaggct
     4501 cgcatcgccg catgggtatg gcgcacgccc tcctgaacgt gggcggcctc acgctcaacg
     4561 tcgcttcgct ggcgttgagg gtaggcggca agaagaatcg cggcgtggcc cggtccctct
     4621 cggcgggcgg ctacgtgctc aacgcggcgg cggcatacgt tgcgggcgag ctggtgtaca
     4681 acctcggtca ggccgtcaat cgcgacgcct gggtggacgg ccccgacaag ttcaccgacg
     4741 tggcctccct cgaagacctg cgggaaggcg agatggtcaa ggtggacgta gacgggaggc
     4801 ccgttgtgct gctacagcac gacgacggca tccacgcctt cgagggcacc tgcccgcact
     4861 acggctgcgg cctgtgggag ggcatcctgg aggaccacac ggtcacctgc cagtgccacg
     4921 gctcgcaatt cgacgtgcgc gacggcactc tcatccacgg ccccgccacc gcccccgtcc
     4981 ccagctacga cgttcgcagg cgcatgggcc gcgtgcagat acgcatgcgt aagtgacgag
     5041 ttgttagttg ttagttgtta ctgacggggt tggtacagcg agtgcgcgga tgtgaggggc
     5101 acatagtgca gcgaccccac tcccccttgt catcctgagc gtagcgaagg atcaggtgg
//
LOCUS       DASRWZ010000216         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_771225, whole genome shotgun sequence.
ACCESSION   DASRWZ010000216 DASRWZ010000000
VERSION     DASRWZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771225"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1979)
                     /locus_tag="VFH60_03325"
     CDS             complement(<1..1979)
                     /locus_tag="VFH60_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312842.1"
                     /translation="MKITRREVAWAVLGSALLATLFLGPAIFTGRIISPADMLYNYYP
                     WRAEQPPDWRGPSNDLLVDSAVQFEPWLEYSARRLHAGELPLWNADNMLGAPLIGNNQ
                     SGIFFPLNWPYLLWPDGWMHTLRLWLKLFIAGLGMYLAAREVAKVGPVGASVATLAFT
                     YGAAMTVWLLYPLTATAMWLPWLWWATARLISQPSARYVAILAGIVALMLLAGHLQTA
                     YHVMLATGLFALFHAWQTAPRQVGRLARTLGLWVIACGLGAMIAAIQLLPFIEYSGES
                     MALLRRSERQAAGGLSLPIEYAWTTFTPDLWGNPARHTWWGTEINYNESHNYIGMMPL
                     ILVPFALFGGDRLKRRMAFLLAVLAALALGVAYHAPVLYDMALTLPGMSLLLNTRMVL
                     VAQFALSLLAALGVEAILRWLPEQHRRVVVGLAVCVGILLAVGVAVPALWAHDFFGVP
                     PFVQQSNSVQYTNLVQQPNLVWQASLWRAAAVIAVCGAVLGAVAALWAIRPRIAHPAM
                     SLVPVVLLADLWLAHAGYNPSVAREEYYPQTAATRFLQEQQRQEDGPFRVIAGGAGPV
                     GVFMPNINLIYGLSDLRGYDALDPKLYVELAVRAAPGGLRATPGGFNTSRMLDLLNVR
                     YVMSAPGDDPNYVVDVRQEASSGVTVGEIAGQTQPG"
     gene            complement(1980..2615)
                     /locus_tag="VFH60_03330"
     CDS             complement(1980..2615)
                     /locus_tag="VFH60_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6312843.1"
                     /translation="MIDRAGAVGSARVAVQLYRGNRFKKLAKPFVVKAISAFWLRFYK
                     VKYGKRVEFGRNFVSRGNLVVRGPGRVVFGDNVNAWSHAEKNVLITFTPDARIIIGDG
                     CRLNGAGIQAYRQVRIGPRCILSSTIIVDTDFHPLDPATRHDHDAPVVCAPITIGENV
                     WVAGQTAILKGVTIGDNSVVGFRADVTKDVPPNVVVGGNPARIVKRLDEGA"
BASE COUNT          538 a          897 c          815 g          475 t
ORIGIN      
        1 cccggctgag tttggcccgc gatctcgccc accgtgacgc cactgcttgc ctcctggcgc
       61 acgtctacca cgtagttggg gtcgtcgccg ggagccgaca tcacgtagcg cacgttgagc
      121 aagtcgagca tacgggaggt attgaaccca cccggcgtgg cgcgcaagcc gccgggagcc
      181 gcccgcaccg ccaactctac gtagagtttg gggtccagcg catcgtagcc acgcaggtcg
      241 gagaggccgt agataaggtt gatgttgggc atgaacacgc ccaccggccc cgcccctccc
      301 gcaatgaccc ggaaaggtcc gtcctcctgc ctctgctgct cctgtaagaa gcgcgtggcg
      361 gctgtttgcg gataatactc ctcgcgggcc accgaggggt tgtaaccggc atgcgccagc
      421 cagaggtcgg ccagcaacac taccggcacg agggacatgg cagggtgagc gatgcgcggc
      481 cttatggccc acaaagcggc taccgcgccc aatactgctc cacaaacggc tataaccgca
      541 gccgctcgcc aaagcgatgc ctgccatacg aggtttggtt gttgaacaag gttagtatat
      601 tgtacagagt tcgattgctg aacaaaaggc ggcaccccga agaagtcatg cgcccacaaa
      661 gcgggcacag ccacccctac agccagcaat attccaacgc acaccgccag cccaaccacc
      721 acccggcgat gctgttctgg tagccaccgc aagattgctt cgacccccaa cgccgccagc
      781 aagctcaacg cgaattgcgc taccagcacc atgcgtgtgt tcagcaacag gctcatccca
      841 ggcagcgtga gcgccatgtc gtacagcacc ggcgcatggt aagccacgcc cagcgccagg
      901 gccgcgagca ccgccagcag gaaagccatc ctccttttca gcctatcccc accgaagagc
      961 gcgaaaggca cgaggatgag aggcatcatg cctatgtagt tatgcgactc gttatagttt
     1021 atctcggtgc cccaccacgt atggcgggcc gggttgcccc acaggtcggg ggtgaaagtg
     1081 gtccaggcgt actctatggg cagggacagg ccgcctgccg cctggcgttc acttcgcctc
     1141 agcagcgcca tgctctcgcc cgaatactct atgaagggga gtagctggat agcggctatc
     1201 atggccccca gcccgcacgc tataacccac agccccagcg tgcgagccag tcgccccacc
     1261 tggcgcggcg cggtctgcca ggcatggaac agggcaaaga ggccggttgc cagcataacg
     1321 tggtaggccg tttgcaggtg ccccgccagc aacatgaggg caacaatccc cgcgagaata
     1381 gctacatagc gggcgctcgg ctggctaatc aggcgtgcag tggcccacca cagccagggc
     1441 agccacatcg ccgtcgcggt cagcgggtag aggagccata ccgtcatggc tgccccatag
     1501 gtaaaagcca gggtggcaac cgacgcgccc acaggcccga ctttcgccac ctcccgcgcc
     1561 gccaggtaca tgcccagccc tgcgatgaag agcttcagcc agagtcgcag ggtgtgcatc
     1621 cacccgtccg gccacagcaa gtagggccag ttgaggggga agaagatgcc cgactggttg
     1681 ttgccgatga ggggcgcgcc gagcatattg tcggcgttcc acaggggcag ttcgcctgcg
     1741 tgcaaccggc gggcgctgta ttccagccag ggttcaaact gcaccgcgct gtcaaccagc
     1801 aggtcgttgc tcggccctcg ccagtcggga ggctgctcgg ctcgccaggg gtagtaattg
     1861 tagagcatgt cggcgggcga gatgatgcgc ccggtgaata tggccgggcc gaggaagagt
     1921 gtggcgagga gagcagagcc taacaccgcc caggccactt ctcggcgggt tatcttcaac
     1981 tacgctcctt cgtccaggcg cttgactatg cgcgccgggt tccccccgac caccacgtta
     2041 ggcggcacgt ccttagtgac gtcggcgcga aagcccacca cagaattgtc tcctatcgtc
     2101 acgcccttga gtatggccgt ctgcccggct acccacacgt tctcaccgat agtgatgggc
     2161 gcacacacca ccggcgcgtc atgatcgtgc ctcgtcgcgg ggtcgagcgg gtggaagtcg
     2221 gtatctacga taatggttga gctgagtatg cagcgcgggc cgatcctgac ctgcctgtag
     2281 gcctgtatgc ccgcgccgtt caaacggcaa ccgtcgccta taatgatgcg tgcatcaggc
     2341 gtaaaagtga tgagcacgtt cttctcggcg tggctccacg cgttgacgtt atccccgaac
     2401 accacccggc ccggccctct gacgaccagg ttgcctctgc tgacgaagtt gcgcccgaac
     2461 tccactcgct tgccgtactt cactttgtag aatcgcagcc agaacgcgct gatagccttc
     2521 accacgaaag gcttggccag cttcttgaac ctgttgcccc ggtagagttg gacggcgacc
     2581 cttgccgaac cgaccgcacc ggcacggtca atcacccagc gcctcctggg gtgtatcgct
     2641 tatgcgcagc atggaggtat cgggcgctct gttgcctgct ctgcgtgccc cggccctctc
     2701 gtatactgcc tctatacggt caatg
//
LOCUS       DASRWZ010000217         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_17450,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000217 DASRWZ010000000
VERSION     DASRWZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17450"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFH60_03335"
     CDS             complement(<1..213)
                     /locus_tag="VFH60_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312844.1"
                     /translation="MRKSREPLDRSDVISIISLLVALFGTLFSGIFAMLAQQSAEEAN
                     RIARDANTPSVSASAEWQIVNIELQLC"
     gene            353..1252
                     /locus_tag="VFH60_03340"
     CDS             353..1252
                     /locus_tag="VFH60_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312845.1"
                     /translation="MANSTILYACTERGLGIVNKPGTSTEWLPLRVVLPDRAVVSAWA
                     DPGPPIRVMVVDAGGELLLSENGGRTWSPVPGLPSVASLFEVGDPPLPHVITQAGMIA
                     RTNDGGTTWETLPFAPREVQVSPGAHATGAGEYVYLSGTVEGYPSALRGNLGNGDWAD
                     IGLEEVAALAHDADKGGLYACGLLGVYAGAGSSWTLLPGSPRDGTSIVAVPGPAARPR
                     TLLVGTPTGLSVSPDANAWQTADLPHEGAITALARDPERRDRVYAATSTGYLLESGNR
                     GQSWQPVNSNHIALVGSLFVVRI"
     gene            1312..1467
                     /locus_tag="VFH60_03345"
     CDS             1312..1467
                     /locus_tag="VFH60_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312846.1"
                     /translation="MLLSGAKAWRLGLLVVLLVLSEVVARQPAGVYAAQPTPNGGDHP
                     TPVYSTY"
     gene            1945..>3293
                     /locus_tag="VFH60_03350"
     CDS             1945..>3293
                     /locus_tag="VFH60_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET6312847.1"
                     /translation="MNNEQTEQPASVDQQGGNGQGQSNGNANGAYDPALAETASGQDN
                     GQEGAVPTTMAGDMPGPLPEEAAGDTGEADGGADVASADAGAADGTDGADASVAPALD
                     LPADLDTGYVGDAGGAYAEASAASDETDGAMGIGDASDVDAPGAGDTSGVDAMVNTGD
                     TGDTDAAGEGAEATAQVPADGSADSDELVVRTARDPRMESVGANASMEDLLRASEQQY
                     RTLKHNDVIEGTIMRVDRDEILVDIGAKTEGIIPSRELQSLTPEEREALAPGDPILVA
                     VVQPENNEGHALLSLDRARQERSWRDLQRKFEAGEVIQAPVSGHNKGGLLVNIEGVRG
                     FVPSSQSSSLPPGEANKQAELSRYHNQVLPLKIIEINRNRNRLILSERQAMQEQRESM
                     RTRLLRELESGQIRPGTVTSIADFGAFVDIGGADGLIHLSELSWKRVSHPSDVLKVG"
BASE COUNT          607 a         1047 c         1027 g          612 t
ORIGIN      
        1 gcataactgc aactcaatgt tcactatctg ccattctgct gatgcgctga cactaggagt
       61 attggcgtca cgggcaattc tgtttgcctc ttctgccgac tgttgcgcta gcatagcaaa
      121 tatcccactg aaaagcgtac cgaacagcgc taccaaaagg ctgataatgc taatcacgtc
      181 gcttctatca agtggctcac gtgacttcct cattgctcga cacctccgcc tccgactacc
      241 cattctcgtc aatgagaaat atacttctag tgagagcaaa tctttctact actttcatct
      301 tccaattcgt cggtggcgcg ctccctgaaa tacactcatt tgaactgcga taatggctaa
      361 ctctaccatc ctgtatgcat gcaccgaacg cgggctgggc atcgtgaaca agcccggcac
      421 ctctacggag tggctgccct tgcgcgtggt gctgccggac cgggccgtcg tttccgcctg
      481 ggccgaccct gggccgccca tcagggtgat ggtagtggac gccggtgggg agttgttgct
      541 gagtgagaac ggcgggcgta cgtggtcgcc cgtgcctgga ttgccttccg tcgcaagcct
      601 gtttgaggtg ggcgacccac ccctgcctca cgtaatcacg caggccggca tgatagcccg
      661 cactaacgat ggtggcacga cgtgggagac cctgcccttc gcaccccgtg aggtccaggt
      721 gtcgccgggg gcacatgcta ccggggcggg cgagtacgtc tatttgtcag gcacagtcga
      781 aggctacccc tcagcgttac gcggcaacct gggtaacggc gattgggccg atattgggct
      841 tgaggaggtc gcggccctgg ctcatgatgc ggacaagggc ggtctgtacg cctgcgggct
      901 gctgggggta tacgcaggcg caggctcaag ttggacgctc ttgcccggct cgccccgcga
      961 cggcacctct atcgtggcgg tccccggccc ggcagcccgt ccgcgtacgc tcctcgtggg
     1021 tacccccacc ggcctctccg taagccccga cgccaacgcc tggcaaacgg ccgacctccc
     1081 ccacgagggc gcgatcaccg ccctggcgcg cgaccccgaa cgccgcgacc gcgtctatgc
     1141 cgccacgagc acgggttacc tccttgaaag cggcaaccgg ggtcagtcgt ggcagccggt
     1201 caacagcaac cacatcgcgt tggtaggctc tttgtttgta gtgaggattt agcgtattca
     1261 agcggcaatg aggcgtgtgg gtcctgcctc ttccctggtc accggaggct tatgctgcta
     1321 tctggcgcta aagcttggag gctggggctt ctggtcgtgc tgctcgtctt gagcgaggta
     1381 gtagcgcggc agcccgccgg ggtatatgct gcccagccca cgcccaacgg cggagaccat
     1441 cctacgcctg tctattccac ctactaaggc agcacggatg gggaggagcg acacagcacc
     1501 ggcccccacc cctggcaagg tttaaaggac ttcgacgtgt attgccggaa tacctcgatc
     1561 ggtgcggagc ctggcagccg gtgtccagaa ccacaattat tctccaatcg tacccaggtc
     1621 ctattttacg gtgccatact cgtaagcctt ctgctggtcc ttgccctcgt ccgttcgcgc
     1681 tcatggcggc gcgcacgccc aggcgagtga cgatgggggt tcttaccgtc gcctgtcgtc
     1741 cacctcgcgt gcttgcaatt cagaggggct tctcgtataa tacaaaacga atcgccagat
     1801 tgagaatgcg agaagaactc cccctgtttg ccaataccgg cataatgtcg caccgacgta
     1861 gcgaggtccc ttacctcgcc agcatgtgca gttcccccgc cgacactccc ctggcgtcta
     1921 ctatctcctt tgaggacggt gtggatgaat aacgagcaga cagagcagcc ggctagcgtg
     1981 gaccagcagg gcggcaacgg gcagggtcag agcaacggca acgccaatgg cgcgtacgat
     2041 ccggcgcttg ctgagactgc cagcggccag gacaacggcc aggaaggcgc tgtccctacc
     2101 actatggccg gcgatatgcc cggccccttg ccggaagagg cagcaggcga tacgggtgaa
     2161 gccgacggcg gcgcggatgt ggctagcgcg gatgcaggtg ctgcggacgg cacggacggc
     2221 gctgatgctt cagttgcccc tgcgcttgac ctaccggccg acctcgatac cggctacgtg
     2281 ggtgacgcgg gcggcgctta cgccgaggct tcggctgctt ccgacgagac cgacggcgct
     2341 atgggtattg gagatgcttc cgacgtggac gcaccgggcg ctggtgatac ttccggcgtg
     2401 gacgcaatgg tgaataccgg ggacaccgga gacaccgatg ctgctggtga gggcgcggaa
     2461 gctacagctc aggtcccggc agatgggagc gcggactcgg acgagctggt ggtgcgcacc
     2521 gcacgcgacc ctcgcatgga gtcggtgggc gctaacgctt cgatggagga cctgttgagg
     2581 gcctcggagc agcagtaccg caccctcaag cacaacgacg tcatcgaagg caccatcatg
     2641 cgggtggacc gcgacgagat cctggtggat atcggtgcca agacggaggg catcatcccc
     2701 agccgcgagc ttcagagcct cacccccgaa gagcgagagg cccttgcccc cggcgacccg
     2761 atcctggtgg cggtggtgca gcccgagaac aacgaaggcc acgccctcct ctccctcgac
     2821 cgcgcgcgcc aggagcgttc gtggcgcgat ttgcagcgca agttcgaggc gggcgaggtc
     2881 atccaggccc ctgtttccgg gcacaacaag ggcggcctgc tggtgaacat cgagggcgtg
     2941 aggggcttcg tgccttcttc gcagagttcc tcgctgcctc cgggtgaggc caacaagcag
     3001 gccgagctca gccgctacca caaccaggtg ctgccgctca agatcatcga gatcaaccgc
     3061 aaccgcaacc gcctcatcct ctccgagcgc caggccatgc aggagcagcg cgaatcgatg
     3121 cgcacccgcc tcctgcgcga gcttgagtct ggccagatac gtccgggcac cgttacctcg
     3181 attgccgact tcggcgcgtt cgtggacata ggcggtgcgg acggcctcat ccacctgtcg
     3241 gagctgtcgt ggaagcgggt atcgcacccc agcgacgtgc tcaaggtggg cga
//
LOCUS       DASRWZ010000218        15312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_428619, whole genome shotgun sequence.
ACCESSION   DASRWZ010000218 DASRWZ010000000
VERSION     DASRWZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 15312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15312
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428619"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VFH60_03355"
     CDS             complement(<1..1129)
                     /locus_tag="VFH60_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312848.1"
                     /translation="MTALLNPGIFVGALLACLLVVLCYQARPSYEIVVGSPTDAPLLD
                     GFSGAERVPEGVELGYETFRWLGSYGRVTLSDLGAQPYSVSLTVNGARPPGTSPSLLR
                     AQVGGQVLLDVQPPPNVETYTFAVPHAVVDPQSGSFTLELTSNTFQVAGDSRELGIIV
                     TRIGLQPAPGGPGLIMPVQGVLASVLLAAGLLGVLLAVVGWGPGAVGLGASVPGVLAG
                     GLLVFDRLWLTTGQWYATWPGVALLGGAVLGLAWPAGMWVLRRGGVIWASMERMALLT
                     VLLGAFVVRLAGQLHPQIFVYDLGFHLNHLELVQTGQLLFTNAPAEFGGTGHETFYLP
                     TAYLLAMPLDWLLGDGRLALRVLTVLLGTLGALPVYYIARRL"
     gene            complement(1144..3390)
                     /locus_tag="VFH60_03360"
     CDS             complement(1144..3390)
                     /locus_tag="VFH60_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312849.1"
                     /translation="MKVGRLQFASTGVVKRQPAQAVEPDSARVAAPATPSRLSRLVPH
                     LVVLAAFTLLTIVATWPMFPQLGGYVMDKGDPLYSVWAMAWQAHALATQPLDLFNSNI
                     MYPFRGTLTFDELSFAQAVLSAPFYFVLGNPVLSHNLILFLSFVLSGWATWLLVRELT
                     GSSWAGVVAGAAFAFCFYRINHLPHITLDNTQWMPLVLLAAYKLLWTREWGWAVALGG
                     FFTLQALSGHYLAFYTAMLLGLFVVYYGVAQRRVFSLSFLLKAGAGLAGSLVFILPIA
                     IPYVRDQGQFEFSRSIFEAERFSNTLASFLAVFKGSPAYRSLLAPFADPGPWAIERAA
                     FPGFFVLGLAVAGLVMAMRRGVVVPGEAVKGSLRLHAGFYGLMALISAVLSLGPSLQP
                     WYAPSNYDPAAIQRVMPLPYLFLHEWVPGFQSMRVVTRIGIVTALALSVLAGIGALFL
                     LRYLAGRARGHNVARGLVPVVAALVAFVPVAESWSAPISMQPVGTREAVPAVYKWLGQ
                     QPHTVILEYPMTHYKRGDPSVEMANLYQYYSVYHWHDTINGSTTIRPFAYSALVLETE
                     RCFPCPRSLGALKALGVEYVVAHLANLSGPQLEEFEWRSTHVEGKVLPDFELVQDFGS
                     DRVYRLTGPPDAEELAGQIPPGTSLRLGKLENDPARQENENVAGGYMAAIAFHLRDHP
                     QYGDPRLTFGQPIQAPIEGSRADFGLLWTGEDPAPYGYSAGERLWYNEFVTLYGTHNE
                     TDGGSSVR"
     gene            complement(3387..4622)
                     /locus_tag="VFH60_03365"
     CDS             complement(3387..4622)
                     /locus_tag="VFH60_03365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6312850.1"
                     /translation="MALVFFYPQLKKLTGAERLILKLASSTARALDGREQVVLLTHHI
                     AEECLPALGEGVGLIESGVPQQVTGNHYIDAAVEYVLGPVLALGIPRKKLSGVVFFGP
                     PSVPAMWFARRIWLPLLGKRVPVVYFCFEPPRFIYSDTEDIVSRLGALGRLVSPAFRI
                     YRRLDQAMVRSAHRVLSNSPYGSRRIGNAYKRKAVVIEHGMDFRAPDEGAVEALRERY
                     GLRGRPVAVTVNHLHPRKRIDLFIRAVREAAGRVHGAVALVVGGGPERESLATLAASL
                     GMAVGRDVVFTGAVPEDELPAHYAVGNVYVHTGREESFGLSVIEALGLGLPVVSVNEG
                     GPCDTVQHGKSGYLVPATPEALGGAAADLLADPRTAREMGEAGASFVRSHFQWAKGAA
                     TLLGVLRNVGERLESRKGT"
     gene            complement(4623..4808)
                     /locus_tag="VFH60_03370"
     CDS             complement(4623..4808)
                     /locus_tag="VFH60_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET6312851.1"
                     /translation="MSNEATVPRELLEILVCPVDKAKVTLEGDRLVCEQCGRAYPVRE
                     GIPIMLEEEAEMTGKQS"
     gene            complement(4828..6411)
                     /locus_tag="VFH60_03375"
     CDS             complement(4828..6411)
                     /locus_tag="VFH60_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312852.1"
                     /translation="MTDRQELQSGQTRATPARPDVWRIASWALTGALALLLVGLAVKS
                     GLSAATSAQMWAYPFQFDESEGMIVAETMLLDRGVSIFDVPGPDLFISAPYPPLFYLL
                     NLPGQHLAGAEPTFKVGRTLSILATIFVGITIFGVVVALTRDRLAGAVGAAAWWSLGV
                     VTFWGSLVKPDMTAVALGLAGLWWLAARPERQAWWALVFFLAAFYTKQTAIAAAGAGI
                     LWLLVMRPRTGFGFAGAYAVGAIVPSAILTWLTGGGYYYHQGTFELPGLPDPVHDLPW
                     MPERFALYVSNFVASYWVLLVPGMLAVLLVGLRSLWVRISRRGAKLDVLHRGAILLLA
                     YMGMSVFVSIGTGTLGGNHNHLLDGAAAGGLGLGLAAGMARRAEYLGVKLAGVGVALV
                     AIIALPSLFATPEWLRLSFSPLEPGKYEGMRNIFQYVTNNGGPAYSDNVGLLLTTGKR
                     LWTTDPYTQTHATQYGRWDESKLVAEIRRKGFAQVILRIDIFAPDPGAGDVSPGILQA
                     LRDNYKLDQRNVENIYVPR"
     gene            complement(6408..8645)
                     /locus_tag="VFH60_03380"
     CDS             complement(6408..8645)
                     /locus_tag="VFH60_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312853.1"
                     /translation="MQLSAAAAGASGMARPPETRLKARSNGTTGTPQPPSAPVDSAPS
                     YSLWKDPLAWALLAVALCMRVLYNLALHPGGHTPLDFVIDEREYFSAAHVFAEGRGFA
                     FYNDFIWVRPPLYVLFLGGLFGLGGGSYQYGPVLLAQSVISAASLLPFGLLAGEVGGR
                     AGARWTLGLGVLYLPFALFVGLLVSETLFLGFFGFALLAVWQGRQALVRGEARRGYLW
                     LAGAGLLLGCATLTRSTAIAFIFVAALWLLLSLPRAWTPGRKLVPSAVLVLVALATLV
                     PWAVRNYSAYHRFIPVDTTGGYNLWLASLGVRDEPRMQEVLRRVEGQGERQDYAFARA
                     WETITADPAAFVGKGLKESLDLWKPSLSAEENAVRGYPSGRVPAWHMALLLLLDNVLY
                     IVILLLAICGFALTKPTPLKWLTGAWVLLWVVMSFIFFAVTRFRMPVVASLLPWAGVG
                     MAALPSLGDWRESSLLARVSSAVAVLAIVVVVGLNVPVEDTLKGLDAWQRQAPYREGE
                     RLLGEGRVPEAVESYRRADLNVADTRYGLAAALLQLGSVDEAMSVLRPNEPGDRYEPP
                     ILGGQAARMRGNLDTARSFFNARVVQVAGDEALRWAWDHLRPESVQSLELGSGLDVGY
                     VRGFHGPEKDANGRSFRWSIDRAEVRGISGGLCTLDWNGWRPSGLPPAEVTLTPTNSQ
                     GNDMSGLMKIALSNSPEWSATRIPSPPTGGISGLVLEVDSFVVSGSDPRLVGVQLAWV
                     SGCQR"
     gene            complement(8569..9852)
                     /locus_tag="VFH60_03385"
     CDS             complement(8569..9852)
                     /locus_tag="VFH60_03385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6312854.1"
                     /translation="MKILMVTSSYPKYEGDVTAPFIESIAASIAARGNEVHVLAPYHP
                     DLKRAPVEKGVHLHFFKYAPLRHLNIWGYAEALEADVRVKGAIFPLTPVVFASSLWSL
                     FQLTRQVKFDIMHAHWVVPNGPVAALVARLRRLPLVVSLHGSDIFIAEHSRLIASIAR
                     RVFRAASAITAPSDDLRERATKIGAPANRCHLVPYGVDPSKFTRIEGAGPLLRKELGL
                     PEDAVVIFAVGRMVYKKGFEYLIRALPAVLREHPNARIVLAGGGDLESKLVSLVRQLG
                     VEKSVIMPGWVSRDKLPLYFSGCDLFVLPSVVDQQGNVDGLPNTLLEAMASARPVVAT
                     NVAGIPLAVKDGENGLVVPEKQPGELSAAINLLLRAPDLRQQYGQAGRRRVEDDLNWD
                     TTARTFENLYHAAFSRRRRSKRHGEAAGNQIEGAQ"
     gene            complement(9973..10326)
                     /locus_tag="VFH60_03390"
     CDS             complement(9973..10326)
                     /locus_tag="VFH60_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6312855.1"
                     /translation="MLENNSSPHGRARLMLAVFFCLLALLVPVVLAACDSGGDAGATP
                     RSGEVVFGRYCNTCHPGGGLGAGPSLIRSVPDLSDDQLRDIVRHGKSRMPGFTTHEIS
                     DAELDDLIGFVRGLK"
     gene            10935..11075
                     /locus_tag="VFH60_03395"
     CDS             10935..11075
                     /locus_tag="VFH60_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312856.1"
                     /translation="MDQHSYEHNVPQQLVGFAATGLGKVALTLSTLSLLIGGLLVSVC
                     AR"
     gene            complement(11253..14438)
                     /gene="ileS"
                     /locus_tag="VFH60_03400"
     CDS             complement(11253..14438)
                     /gene="ileS"
                     /locus_tag="VFH60_03400"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET6312857.1"
                     /translation="MAFKPVDSRPDFVAMEHAILEFWRETDAFRKLKEKNKGNKHWSF
                     IDGPITANNPMGVHHGWGRTFKDIAQRYRAMLGYDQRYQNGFDCQGLWVEVEVEKELG
                     FASKRDIEAFGLDRFSRACRERVNKYAGIITRQSERLGQWMDWDDSYYTMSDNNMEHI
                     WLFLQKCHENGWLYRGTRSMHWCWRCGTSLSQHELTDNYRDVTHTSVYLKFPLTTPGH
                     EGESLLIWTTTPWTLAANVAAAVHPELEYVKVRQGEEVHYISRGAIGRLAGEYEEVGT
                     VKGAELVGLTYSGPFDELPAEEGVHHPVIAWDEVQDAEGTGIVHIAPGCGAEDFELSK
                     VYGLAVLVPLDEAGSYTGKFGWLSGQHVTEVAPRIFDSLRDKGVLYNLEDYTHSYPTC
                     WRCNTELVFRLATEWYIGVDEVRPRMRRAAGEVKWTPDYAGKRMDDWLTNMGDWNISR
                     KRFWGLTLPFYVCAHCDTVTVVNSRQELRDRAVNPDVVDALPELHRPWVDEVKIKCPN
                     CAETVSRIPEVGDAWLDAGIVAFSTLDYRSNPEYWRKWFPAHWVSEMREQIRLWFYSM
                     LFMSVTLEDTAPYEQVLTYEKVHDEKGRAMHKSWGNAIWFDEAAEKMGADVMRWLYAG
                     ANITQNMNFGYNLAEEVKRNLLTLWNTYSFFVMYANLDGFDPSASAVPVRERAELDRW
                     ILARLHELIQLCRTELDSFDTASVTRNVDAFVDDLSNWYVRRSRRRFWKSESDSDKLS
                     AYSTLYEVLVTLSKLVAPVMPFLAEEMYQNLVVRGGNSNGQPESVHHNDYPEADESLI
                     DRQLLADTALAQRIVSLGRAARNKEGVKVRQPLREMVVRAPSRRDEEAVERIVQQVLE
                     ELNVKNLTLTNQVGELITFVIKPNFALLGPKYGARLNAIRQALAKADPAEVAASVGQE
                     QPVRVQLEGEEGSVELLPAELVVETREREGFAVAQEGGLVVALDTHLDEPLLQEGMAR
                     DLVRVINDMRKSADFDVSDRITTYFRLNGEGGSANGEVNLLRGAVANFGEYIRAETLS
                     NALVEGAAPEGAFTQEEQLGGAAISLGVERK"
     gene            complement(14525..>15312)
                     /locus_tag="VFH60_03405"
     CDS             complement(14525..>15312)
                     /locus_tag="VFH60_03405"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HET6312858.1"
                     /translation="RTARGLGLRPSLAPVAQQQQQPTRQEAPTPPERRAQAVTELTPV
                     AEAAVESSRSQATEPADAPEPEVAAEEAPAAPTPDISSLMKRGTDVAAKPRSEQRKPK
                     PEPEAPPEPEAPAEEAEEEEPAEQSNLMELQVVFDGGSRGNPGEGYGSFLVQAPGRKP
                     VIKRLEFGDNYTNNQAEYESLIGSLKYIIERLNTTGRTPDQVQLDIKTDSDLVVNQIL
                     GNFKVKDAGLRKRHEQATDLLGQFADWAISWHPRDVSVKLLGH"
BASE COUNT         2787 a         5321 c         4558 g         2646 t
ORIGIN      
        1 tcagacgcct ggcgatgtaa tacactggca gcgcgcccaa cgtgccgagc aggaccgtca
       61 gcacccgcag cgccagcctg ccgtcgccca gcagccaatc gaggggcatt gcgagcaagt
      121 acgcggtggg caggtagaag gtctcgtgcc cggtgccgcc aaactcggcc ggggcgttgg
      181 tgaaaagaag ctgcccggtc tgaactagct ccaggtggtt caagtggaag ccgaggtcgt
      241 acacgaatat ttgcgggtgt agctgccccg ccagcctcac cacaaacgcg ccaagcaaca
      301 cggtaagcag cgccatgcgc tccatactcg cccagatcac cccgccccgt cgcagcaccc
      361 acatgcccgc cggccacgca agccccagca cggccccgcc caacagcgcc acgcccggcc
      421 acgtcgcgta ccattggccc gtagtgagcc acagccggtc aaataccagc agcccgcccg
      481 ccagcactcc cggcacactc gccccaagcc ccaccgcgcc cggaccccac cccacgactg
      541 ccagcagcac ccccagcaaa cccgccgcga gcagcactga tgccagcact ccttgcaccg
      601 gcattattaa ccctggcccg cccggtgccg gttgcagccc gattcgcgtc acaataatgc
      661 ctagctcgcg actatcgccc gccacctgga aagtgttgct ggtaagctcc agtgtgaagc
      721 tgccactttg aggatcgact accgcatggg gcacggcgaa cgtataagtc tccacgttgg
      781 gaggcggctg gacatcgagc agcacctgcc cgccgacctg cgcgcggagc agtgagggtg
      841 aggtgccggg cgggcgagcg ccattgacgg tgagggacac ggagtagggc tgcgctccca
      901 ggtcgctcaa agtcacgcgc ccgtagctgc ccagccagcg aaaggtctcg tagcccagct
      961 caacgccctc cggcacgcgc tccgccccgc tgaagccgtc gagcaggggc gcgtccgtgg
     1021 gcgaacctac aacgatctca tacgatggcc gggcctggta gcacaggacg accagcaggc
     1081 aagcgaggag ggcacccaca aagatgccgg gattgagcaa ggctgtcaac aggcggcctc
     1141 gtctcaccgc acgctgctcc ccccgtcggt ttcgttgtgc gtcccgtaga gcgtgacaaa
     1201 ctcgttgtac cagagccgct cgccagccga gtagccataa ggagccgggt cctcccccgt
     1261 ccacagcaac ccgaagtcgg ccctgcttcc ttcgatggga gcctgtatgg gctggccgaa
     1321 ggtcaggcga gggtcgccgt actgcgggtg gtcgcgcagg tggaaggcaa tcgcggccat
     1381 gtacccacca gccacgttct cgttctcctg ccgcgccggg tcgttctcta gcttgccaag
     1441 cctgagcgac gtgccgggtg gtatctgtcc tgccagctcc tcggcgtcag gaggcccggt
     1501 cagccggtag acgcggtcgc tgccgaagtc ctgcaccaat tcgaagtcgg gcagcacctt
     1561 gccttcgacg tgcgtcgagc gccactcgaa ctcctcaagc tgcggcccgc tcaggttcgc
     1621 caggtgcgcc accacgtatt cgacacccaa cgccttgagc gcgccgaggc tgcgagggca
     1681 cgggaagcac cgctcagtct cgagcaccag cgccgagtag gcgaaggggc gaatagtggt
     1741 gctgccgttg atggtgtcgt gccagtggta cacgctgtaa tactggtaca ggttcgccat
     1801 ctccacgctg gggtcgccac gcttgtagtg ggtcatcggg tattccagga tgacggtgtg
     1861 gggttgctgg cccagccact tgtagacggc aggcaccgcc tccctggtgc ccaccggctg
     1921 catgctgatg ggcgcgctcc agctctctgc cacgggcacg aacgctacca gcgccgccac
     1981 gaccggcacc aacccgcggg ccacgttatg ccccctcgcg cgtcccgcca ggtacctcaa
     2041 caggaacaaa gcgcctatgc cggcaagcac cgagagcgcg agcgccgtta caatgccaat
     2101 cctcgtgacc acccgcatgc tctgaaagcc gggcacccac tcgtgcaaaa acaggtacgg
     2161 cagcggcatc acgcgctgga tagcggcggg gtcgtagttc gagggcgcgt accagggttg
     2221 caggctcggc cccagcgaca gcacggcgga gatgagcgcc atcagcccgt agaaccccgc
     2281 atgcaacctg agcgagcctt ttaccgcctc gcccggcaca actacgcccc tccgcatcgc
     2341 catgacaagg cccgcgaccg ccagccccag cacgaagaag ccggggaagg ccgcgcgctc
     2401 tatcgcccac gggcctgggt ccgcgaaagg agccaacaac gagcggtaag cggggctgcc
     2461 cttgaacacg gccaggaagc tagccaatgt attggagaag cgttccgcct cgaagatgct
     2521 gcggctgaac tcgaactgcc cctggtcgcg cacgtacggg atggcgatgg gcaggatgaa
     2581 cacgagcgag cctgccagcc cggccccggc tttcagcagg aaggagagag aaaatacccg
     2641 gcgttgcgcg acgccgtagt acacgacgaa tagccccagc agcatcgccg tgtagaaggc
     2701 aaggtaatgg ccggagagcg cctgcaacgt aaagaagcct cccagcgcca ccgcccaacc
     2761 ccactctcgc gtccatagca gcttgtaggc ggccagcagc accagcggca tccactgcgt
     2821 gttgtccagc gtgatatggg gcaggtgatt tatgcggtag aagcaaaacg cgaacgccgc
     2881 gcccgccacc acccccgccc agctactgcc ggtaagctcg cgcaccagca gccacgtagc
     2941 ccagcctgac agcacgaagc tgaggaacag tatcaggttg tggctgagca ccgggttgcc
     3001 aagcacgaag tagaacgggg ccgacaacac cgcctgcgcg aacgaaagct cgtcaaaggt
     3061 gagcgtgccc cggaaggggt acatgatgtt gctgttgaac aggtccagcg gctgggtcgc
     3121 gagtgcgtgc gcctgccagg ccatcgccca cacgctgtaa agcgggtcgc ctttgtccat
     3181 gacgtaccca cccaactgcg ggaacatcgg ccaggtggcg acaatcgtga gcagggtaaa
     3241 ggcggccagc acgaccaggt gcggcaccag ccttgacagc ctggaagggg tcgcgggcgc
     3301 ggcgactctg gcgctgtcag gctcgacggc ctgggccggt tggcgcttga ccacgccggt
     3361 ggatgcgaat tgcagacgac ctactttcat gtacccttcc ggctctccag gcgctcccct
     3421 acattcctca gcacacccag cagcgtagcg gcacctttcg cccattggaa gtggctgcgc
     3481 acgaagctag caccggcctc gcccatctcc ctcgcggttc gcgggtccgc aagcaggtca
     3541 gccgccgcgc cacccagcgc ctcaggcgtg gcgggcacaa ggtagccgct cttgccgtgc
     3601 tgcaccgtgt cgcaaggccc gccctcgttc accgacacca ccggcagccc aagccccagc
     3661 gcctctatca ccgagaggcc gaagctctcc tcacgccccg tgtgcacgta gacattgccc
     3721 actgcgtagt gcgctggaag ttcgtcctcc ggcaccgccc cggtaaacac cacgtctctg
     3781 cccaccgcca tgcccagcga tgcggcaagc gttgcaagcg actcccgctc aggtccgccg
     3841 cctactacca gcgccaccgc accgtgcacc ctccctgccg cctcgcgcac cgcccggatg
     3901 aagaggtcta tccgctttcg tgggtgcagg tgattcaccg tgactgcaac cggcctgcct
     3961 cgcaacccgt atcgctcccg caatgcctcg accgcgccct cgtccggcgc acggaagtcc
     4021 ataccgtgct cgatcaccac cgccttacgc ttgtaagcgt tcccgatgcg cctcgacccg
     4081 taagggctgt tcgacaacac ccggtgggcc gagcgtacca tcgcctggtc cagccgccgg
     4141 tagatgcgaa acgccggtga caccaggcgg cccagcgccc ccagccgtga gactatatct
     4201 tctgtgtcgc tgtagatgaa gcggggcggc tcaaagcaga agtagactac gggcacccgc
     4261 ttgccgagca ggggcagcca tatcctccgg gcgaaccaca tggcgggaac gctcggcggt
     4321 ccgaagaata ccaccccgct gagcttcttg cgcgggatgc ccagggccag gacggggcca
     4381 agtacgtact ccacggcggc gtcgatatag tggttgcccg tgacttgttg cggtacgccc
     4441 gactctatta gccctacccc ctcgcccagc gcgggcaggc attcctccgc gatgtggtgg
     4501 gtcagcaaga ccacctgctc tcgaccgtcg agcgccctgg ccgtagagct tgccagcttc
     4561 aggataagtc gctcggcccc cgtgagcttc tttagctggg ggtagaagaa gaccagggcc
     4621 aatcagcttt gcttcccggt catctcggct tcttcttcca gcatgatcgg tatgccttcg
     4681 cgtaccgggt acgccctccc gcactgctcg cacaccagcc ggtcgccctc cagcgtcacc
     4741 ttagccttat ccaccgggca gaccagtatt tccagcaact cgcgcggcac cgtggcctcg
     4801 tttgacatag cgctcactcc tgtgcgttca cctgggaacg taaatattct cgacgttgcg
     4861 ttgatcaagt ttataattat cccgcagcgc ctgcaagatt ccgggcgata cgtcgcccgc
     4921 gccggggtcg ggagcaaaga tgtctatccg cagtatcacc tgcgcgaagc ccttgcgccg
     4981 tatctcggcc accagcttgc tctcgtccca ccgcccgtac tgggtggcgt gagtctgggt
     5041 gtacgggtcg gtggtccaga gccgcttgcc ggtggtcagc agcaggccga cgttgtccga
     5101 gtaggccggg ccgccgttgt tggtcacgta ctggaagatg ttgcgcatcc cctcgtactt
     5161 gccaggctcc aacgggctga acgagagccg cagccattcg ggagtagcaa agagggatgg
     5221 cagcgcaatt attgccacca gggccacgcc cacccccgcc agcttcacgc ctaggtactc
     5281 cgcgcgacgc gccatacccg ccgccaaccc cagccctagc cctcccgccg ccgccccgtc
     5341 gagcaggtgg ttgtgattcc cacccagcgt gcccgtgcca atggagacaa agacgctcat
     5401 acccatgtag gccagcagca ggatagcgcc ccggtgtagc acatctagct ttgccccccg
     5461 ccgcgaaata cgcacccaca gcgagcgtaa ccccacaagc agcaccgcga gcataccagg
     5521 caccagcaac acccagtagc tcgccacgaa gttgctgacg tagagcgcga aacgctccgg
     5581 catccagggc aggtcgtgca cggggtcggg cagacccggt aattcgaacg tcccctggtg
     5641 gtagtagtag ccgccgcccg tcagccaggt gagaatggcg cttgggacga tggcgcccac
     5701 cgcgtacgct cccgcgaatc cgaatcccgt gcgaggtcgc atcacaagca gccagaggat
     5761 gcccgccccc gccgccgcaa tcgccgtctg cttggtgtag aacgccgcca ggaagaagac
     5821 gagcgcccac cacgcctgcc tctccggccg cgcggcgagc caccacagcc ccgctaggcc
     5881 gagcgccacc gccgtcatgt ccggcttgac caggctcccc cagaacgtca ccacacccag
     5941 cgaccaccag gcagcagccc caacagctcc cgccagcctg tcccgcgtga gggccaccac
     6001 gacgccgaag atcgttatac caacgaagat ggtcgccagt atggagagcg tgcggcctac
     6061 tttgaaggta ggctcggccc ccgccaggtg ctgtccgggc aggttgagca ggtaaaacag
     6121 cggcgggtag ggcgcggaga tgaagaggtc ggggccgggc acgtcgaaga tagacactcc
     6181 ccggtcgagc agcatcgtct cggctacgat catgccctcc gactcatcga actgaaacgg
     6241 gtaagcccac atctgcgcgg acgttgcagc cgacaggcca ctcttcaccg ccagccccac
     6301 gagcagcagt gccagggcac ccgtcagcgc ccagctagct atcctccaca cgtccggcct
     6361 ggccggtgtg gccctggtct gcccgctctg tagctcctgc cggtccgtca ccgctggcac
     6421 ccgcttaccc aggcaagctg cactcccacc agtcgagggt cgctacccga cacgacgaag
     6481 gagtccactt caagtacgag accgctaatt ccaccggttg gaggcgatgg tatcctggtg
     6541 gccgaccact caggggagtt cgagagcgct atcttcatta gcccggacat atcattcccc
     6601 tgcgagttcg ttggtgtcaa agtcacctcg gccggaggca acccggacgg tcgccagccg
     6661 ttccagtcga gggtgcacag tccgcccgat atgccccgca cctccgccct gtcgatgctc
     6721 caccggaagc ttctgccgtt cgcgtccttt tcggggccgt gaaagcctct cacataccct
     6781 acatcaagac cgctgcccag ttcgaggctt tgcacgcttt cgggccgcag gtggtcccac
     6841 gcccagcgca gcgcctcgtc ccccgccacc tgcaccaccc tcgcattgaa gaacgagcgg
     6901 gcggtatcga ggttgccccg catgcgcgcc gcctgcccgc ccaggatggg aggctcatag
     6961 cggtcgcccg gctcgttcgg cctgagcacc gacatggctt cgtccacgct ccctagttgt
     7021 agcagggcgg ctgcaagccc gtagcgcgtg tcggcaacgt tcaggtcggc ccgccggtag
     7081 ctctcgaccg cctccggcac gcgtccctcg cccagcagac gctcgccttc cctgtatggt
     7141 gcctgccgct gccacgcgtc aaggcccttc aacgtatcct caaccggcac gttcagaccc
     7201 acaactacca ctatcgccag cacagcaact gcggacgata cgcgcgcgag taggctagac
     7261 tctcgccaat cgcccaggct cggcaaggca gccatcccca cccccgccca cggcaacaga
     7321 gatgcgacca cgggcatgcg gaagcgcgta acggcgaaaa atatgaacga catcaccacc
     7381 cacagcagca cccacgcgcc cgtgagccac ttcagcggcg tgggcttcgt cagcgcgaac
     7441 ccgcatatcg caagcagcag aatgactatg taaagcacgt tgtcgagcaa cagcaggagc
     7501 gccatgtgcc acgcgggcac cctccccgat gggtagccgc gcaccgcgtt ctcctccgcg
     7561 ctgagtgacg gcttccacag gtcgagcgac tccttcagcc ccttgcccac gaaggcggcg
     7621 gggtcggcag taatcgtctc ccaggcgcgg gcgaatgcgt agtcctgccg ctcaccctgc
     7681 ccctctactc tgcgaagcac ctcctgcatc ctcggctcgt cgcgcacccc aaggcttgcc
     7741 agccagaggt tgtacccgcc cgtcgtgtcc acggggatga agcggtggta cgccgagtaa
     7801 ttgcgtaccg cccagggcac cagcgtagcc agggccacca gcaccagcac ggccgaaggc
     7861 acgagcttcc tacccggcgt ccaggcgcgc ggaaggctaa gcaagagcca gagcgcggcg
     7921 acgaaaatga acgcaatcgc ggtggagcgc gtaagagtgg cgcagccgag taacaggccc
     7981 gcaccggcca gccaaaggta gcccctgcgc gcctcgcccc gcaccagcgc ctgacgcccc
     8041 tgccacacgg ccagcagggc aaacccgaag aaccccagga acaacgtctc cgagacaagc
     8101 agccccacga acagcgcgaa cggcaggtac agcaccccca gccccagcgt ccaccgcgcc
     8161 cccgccctac cccccacctc ccccgccagc agcccgaacg gcaggagcga tgcggcgcta
     8221 atcaccgatt gcgccaggag caccggcccg tactggtaac tgccaccgcc taacccaaaa
     8281 aggccgccca ggaacagcac gtagagaggg ggccgcaccc agatgaagtc gttgtagaag
     8341 gcgaagccac ggccttctgc gaatacgtgg gccgcgctga aatactcgcg ctcgtcaatg
     8401 acaaaatcga ggggtgtgtg cccgccgggg tgtaaagcga ggttatagag cacgcgcatg
     8461 cagagggcca cggccagcag cgcccatgcg agcgggtctt tccacaacga gtaggagggt
     8521 gcgctgtcta caggcgcgga cgggggctga ggcgtgcctg tggtgccgtt actgcgcgcc
     8581 ttcaatctgg tttccggcgg cctcgccatg ccgcttgctc ctgcggcggc ggctgaaagc
     8641 tgcatggtag aggttctcga aggtgcgcgc ggtggtgtcc cagttcagat cgtcctcgac
     8701 cctgcggcgg ccggcctgcc cgtattgctg gcgcaggtcg ggtgccctca atagcaggtt
     8761 gatggccgcc gatagttccc ccggctgctt ttcgggcacc accaggccgt tctccccgtc
     8821 cttcaccgcc agcggtattc ccgctacgtt ggtagccacc accgggcggg ccgaggccat
     8881 cgcctcaagc aacgtgttgg gcaggccatc gacgttcccc tgctggtcca ccacggaggg
     8941 cagcacgaac aggtcgcagc cgctgaagta gaggggcagc ttgtcgcggg acacccagcc
     9001 cggcatgatg accgacttct ctacgcccaa ctgccgcacc agcgacacga gcttgctctc
     9061 caggtcgccg cccccggcca gcacgatgcg ggcgttcggg tgctcgcgca gcacggcagg
     9121 caaggcccgt atcaggtact cgaacccctt cttgtagacc atgcgcccca ccgcgaagat
     9181 gaccaccgcg tcttccggca gccccaattc cttgcgcaac aacggcccgg ccccctcgat
     9241 gcgggtgaac ttgctggggt ctacgccgta gggcaccagg tggcagcggt tggcgggggc
     9301 gccaatcttc gtggccctct ctctcaaatc gtcgctgggt gcggtgatgg cgctcgcggc
     9361 cctgaagacc ctgcgcgcga tggaggcgat gaggcggcta tgctccgcga tgaaaatatc
     9421 ggagccgtgc aagctgacca cgagcggcag ccttcgcaga cgcgccacca gcgccgcgac
     9481 cgggccgttg ggcaccaccc agtgagcgtg catgatgtcg aacttgacct gccgggtaag
     9541 ctgaaacagc gaccaaaggc tgctggcaaa taccacgggg gtgaggggaa agatagcccc
     9601 cttgacccgc acgtcagcct ccaacgcttc cgcgtagccc caaatgttga ggtgcctgag
     9661 gggggcgtac ttgaagaagt gcaggtgcac gcctttctcc accggggcgc gcttcaggtc
     9721 ggggtggtaa ggagccagca cgtggacttc gttgccacgt gcggcgatgc tggccgcaat
     9781 cgactcgatg aacggtgcgg taacatctcc ctcgtacttg ggataggagc ttgttaccat
     9841 cagtatcttc atagctctgg gtgattccta cgcagggttg gtctccgtcc accggccggg
     9901 cacgctgacc attatacagc atatacccgc cgcccgccgc agggcacgcc tggctagcgc
     9961 gccggcccgt agctacttca acccgcgcac gaagccgatg aggtcgtcca gctctgcgtc
    10021 ggagatctcg tgcgtggtga agccgggcat gcgcgacttg ccgtgccgca cgatgtcgcg
    10081 tagctggtcg tcggagagat cagggacgga ccgaatgaga gaaggccccg cgcccagccc
    10141 cccgcccggg tggcaagtgt tgcagtaccg cccgaacacc acctcgcccg accggggagt
    10201 cgcacccgcg tccccgccac tatcacaagc cgccagcacg acgggcacga gcagggccag
    10261 caaacagaag aataccgcca gcatgaggcg cgcgcggccg tgaggtgatg aattgttttc
    10321 gagcatgggg tgtctcttct gagaagaagt gggagctata ggtggggtgc cagcgttgcc
    10381 cctgacgccg cacatattgt acattcgtgc cttgtctcgg tcaagcacgg gtatcccaga
    10441 tgacgggtgt aactatattc ggcaaaggtg cagtgggggt gccctgttgg tatgccctgc
    10501 ccacctcgcc ctgtgggcac gccacgcaat gaattggtgg gcttacacga ggcaaagtcc
    10561 cctacgggaa ctaagcaaca agagggtagt ctccgaagga gacttcgtct ctggtcagcc
    10621 cgccaattca ttggcagggc gaggtgggca gggcgtccat gcagagcaac ctacccctca
    10681 actgcagaaa ccccacagaa gatttactta ttcggcctat ccgaccccta tcctttgcgc
    10741 ggacgtgtcg ttataacctg tggaacggct tgatgagtgc gcccctcatc tctccgtcaa
    10801 gggcagccgg ggcatatacg cccgatttac gagtgcattt gcctcggcca gcccagacct
    10861 tctcctcccg cactcttact cacttcatag acaacattgt cgcataccgt cgcaccccac
    10921 aagagggtaa tatcatggac cagcactctt acgagcacaa tgtcccgcag caacttgtag
    10981 gcttcgccgc caccggcctc ggtaaggtgg ccctcaccct gagcaccctc agccttctga
    11041 tcggcggcct cctcgtttcc gtctgcgctc gctagtaaat agacgaaacc gccaagaaag
    11101 tataggtgtg gctacactgg aaagaccata gaacgaactc acgtactctg gtctttctgc
    11161 ttagaacacc tacttaatct tggcgcgctc ttggcgttct tggcgtccct tcggctacgc
    11221 tcagggcatg cttggcggtt tcgtctattt agctacttcc tctccactcc caggcttatg
    11281 gccgccccgc cgagttgctc ctcttgcgtg aaggcacctt ccggcgcggc cccctccacc
    11341 agggcgttcg acagggtctc ggcccggatg tactcgccga agttcgcgac cgcgcctcgc
    11401 agcaggttca cctccccgtt tgcgctccca ccctcgccgt tcaggcggaa gtaggtcgtt
    11461 atgcggtcgc tcacgtcgaa gtcggccgac ttgcgcatat cgttgatgac gcgcaccagg
    11521 tcgcgggcca taccttcctg cagcaacggc tcatccaggt gcgtatcgag cgccaccacc
    11581 aggccaccct cctgcgctac cgcgaagccc tcccgctcac gcgtctccac gacaagctcg
    11641 gcgggcagca gctctaccga cccttcctcg ccttccagtt gcacccgcac cggctgctcc
    11701 tggccgaccg aagccgccac ctcggcgggg tcggccttgg cgagcgcctg ccgtatggcg
    11761 ttcagacgag cgccgtactt ggggccgagc agcgcgaagt taggcttgat gacgaacgtg
    11821 attagctcgc ccacctggtt ggtgagggtg aggttcttca cgttcaactc ctcgagcacc
    11881 tgctggacga tgcgttccac cgcctcctcg tcccgcctcg acggggcgcg taccaccatc
    11941 tcgcgcaacg gttggcgcac cttgaccccc tccttgttgc gggcggcgcg ccccaggctc
    12001 actatgcgct gggcgagcgc cgtgtcggcc agtagctggc ggtctatcag cgactcgtcg
    12061 gcctccgggt agtcgttgtg gtgcacgctc tcaggctggc cgttgctgtt gccgcccctc
    12121 accacgaggt tctggtacat ctcttcggcc aggaagggca ttaccggcgc gaccagtttc
    12181 gacagcgtca ccagcacctc gtacaacgtc gagtaggccg acagcttgtc gctgtccgac
    12241 tcactcttcc agaagcggcg gcgcgaacgg cgcacgtacc agttcgacag gtcgtccacg
    12301 aaggcgtcca cgttgcgcgt caccgaggcg gtgtcgaagc tgtcaagctc ggtacggcat
    12361 agctgtataa gctcgtgcag ccgcgccagt atccagcggt cgagctcggc ccgctcccgc
    12421 accggcaccg ccgaggccga tgggtcgaag ccgtccaggt tggcgtacat gacgaagaag
    12481 ctgtaggtgt tccacagcgt gagcaggttg cgcttgacct cctcggccag gttgtagccg
    12541 aagttcatgt tctgggtgat gttggccccg gcgtagagcc agcgcatcac gtcggccccc
    12601 atcttctcgg cggcctcgtc aaaccagatg gcgttgcccc acgacttgtg catggcgcgg
    12661 cccttctcgt cgtgcacctt ctcatatgta agcacctgct cgtagggcgc ggtgtcctcc
    12721 agcgtcaccg acatgaacag catcgagtag aaccagaggc ggatctgctc gcgcatctcg
    12781 ctcacccagt gcgccgggaa ccacttgcgc cagtactccg ggttcgacct gtagtccagt
    12841 gtcgagaagg ccactatgcc cgcgtccagc cacgcatcgc ccacctcggg tatgcgcgac
    12901 accgtctctg cgcagttggg gcactttatc ttcacctcgt ccacccacgg cctgtgcagc
    12961 tccggcagcg cgtccaccac gtccgggttt acggccctgt cccgcagctc ctggcgcgag
    13021 ttcaccaccg tgaccgtgtc gcagtgcgcg cacacgtaga agggcagcgt gagtccccag
    13081 aaccgcttgc gcgatatgtt ccagtcgccc atgttggtga gccagtcgtc catacgcttg
    13141 cccgcgtagt cgggcgtcca cttcacctcc ccggcggcgc ggcgcatcct cggcctgacc
    13201 tcgtccacgc ctatgtacca ctcggtagcg aggcggaaga caagctcggt gttgcagcgc
    13261 cagcaggtcg ggtagctgtg ggtgtaatcc tcaaggttgt acagcacgcc cttgtcgcgg
    13321 agcgagtcga atatcctcgg cgcgacctcg gttacgtgct ggccgctcag ccagccgaac
    13381 ttgcccgtgt agctgcccgc ctcgtccagg ggcaccagca ccgcgaggcc gtacaccttg
    13441 ctaagctcga agtcctcggc gccgcagccg ggggctatgt gcactatgcc tgtgccctcg
    13501 gcgtcctgca cctcgtccca ggctatcacc gggtggtgca cgccttcctc ggcgggcagc
    13561 tcatcgaacg ggccggagta ggtcagcccc acaagctccg cgcccttcac cgtgcccact
    13621 tcctcgtact cgcccgccag gcggcctatc gcgccacgcg atatgtagtg tacctcctca
    13681 ccctgccgga ccttgacgta ctcaagctca gggtgcacgg cggctgccac gttggccgcc
    13741 agcgtccagg gcgtggtggt ccatatcagc aggctttcgc cctcgtgccc cggcgtggtg
    13801 agcgggaact tgaggtagac ggatgtgtgc gtgacatccc ggtagttgtc ggtcagttcg
    13861 tgctgagaca gggacgtgcc gcaacgccag caccagtgca tcgagcgggt gcccctgtag
    13921 agccaaccat tctcgtggca cttctgaagg aaaagccaga tgtgctccat attgttgtcg
    13981 gacatggtgt agtaggaatc gtcccagtcc atccactggc ccaggcgctc cgactggcgc
    14041 gttatgatcc ccgcgtactt attcacccgc tcccggcagg cgcgagagaa gcggtccagc
    14101 ccgaacgcct ctatatcgcg cttggacgcg aaccccagct ccttctcgac ctccacctct
    14161 acccacaagc cctggcagtc gaagccgttc tggtagcgct ggtcgtagcc gagcatagcg
    14221 cggtacctct gggcgatgtc cttgaaggtg cgtccccagc cgtggtgcac gcccataggg
    14281 ttgttagccg ttatcggccc gtcgatgaac gaccagtgct tgttcccctt gttcttctct
    14341 ttcagcttcc tgaaagcatc cgtctcccgc cagaattcga gtatggcatg ctccatcgcc
    14401 acgaagtccg gcctgctatc cacaggtttg aaagccatct cttcctccta ccattttata
    14461 ttttagattt tggattggag aacattcttc taaatccaaa atctaaaatc taaaatccaa
    14521 aatcctaatg tcccagcaat ttcaccgaca cgtccctggg gtgccagcta atggcccagt
    14581 ccgcgaattg gcccagcagg tcggttgcct gctcgtggcg ctttcgcaga ccggcgtctt
    14641 tcaccttgaa gttgcccagg atctggttga ccaccaggtc gctgtcggtc tttatgtcga
    14701 gttgcacctg gtcgggcgtg cggcccgtgg tgttcaacct ctctatgatg tacttcaatg
    14761 aacctatcag gctctcgtac tcggcctggt tgttggtgta gttatcgcca aactcaagcc
    14821 gcttgatgac cggcttgcga ccgggggcct gcaccaggaa cgagccgtag ccctcgccgg
    14881 ggttgcccct gctgccaccg tcgaacacga cctgaagttc catcaggttg gattgttcgg
    14941 caggctcttc ttcctcggcc tcctcggcgg gcgcttccgg ctctggcggt gcttccggct
    15001 ccggcttcgg cttgcgctgc tccgacctcg gcttggcggc cacatcggtc ccccgcttca
    15061 tgaggctcga tatatcgggc gtcggcgcgg cgggggcttc ttcggcagcc acctcaggct
    15121 caggcgcgtc tgccggctcg gtcgcctggc tacgggagga ctccaccgca gcctcggcca
    15181 cgggagttag ctcggtgact gcctgcgctc gcctctcagg gggcgttggt gcctcctggc
    15241 gggttggctg ctgttgctgc tgcgcgacgg gtgccaacga cggtctcagg cccagccccc
    15301 tggcagtacg cg
//
LOCUS       DASRWZ010000219         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_702737, whole genome shotgun sequence.
ACCESSION   DASRWZ010000219 DASRWZ010000000
VERSION     DASRWZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702737"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2427
                     /gene="carB"
                     /locus_tag="VFH60_03410"
     CDS             <1..2427
                     /gene="carB"
                     /locus_tag="VFH60_03410"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET6312859.1"
                     /translation="VSVSDLWQARDFAGRQGFPLVARAAYCLGGTGSGHANDYAELES
                     LITTGLQESPVSSVLLERSVTGWAEIEYEVLRDAVDNAIIVCNMENMDPMGIHTGESI
                     VVAPSQTLSDEDYQRLRTASLEIVRSLGVVGGCNCQFALNQRTGEFAIIEVNPRLSRS
                     SALASKATGYPIARVAAKIALGYTLAELRNEITGASACAEPALDYVVVKLPRWPFDKF
                     RNVDRRLGMTMKSTGEVMAIGRTFEQAFLKALRSLDVGSSWLKVSPTWTEEKLEECLA
                     HATPARPSAIYNALARGWSVDRISALSRIHPWFVTRIAAMCEMEQRYRECPTDAMLLA
                     AKRMGFSDDHLLELEGSAYHSEQERLQLSLQVRRRMSDLGMRPTYKMVDTCAGEFEAS
                     TPYFYSTYEQEDEAGPLPGPKVIVLGSGPIRIGQGIEFDYSSVHAVEALRAAGIKAIV
                     INNNPETVSTDFHLSDRLYFEPLTVEEVLNVVEHEREGLLGVIAQFGGQTALNLINPL
                     VAAGVRILGTSPESISVAEDRDKMAQLCERLGIPAPKWAIVHSHTELETFVPQIGFPV
                     LVRPSYVLGGRGMRVVHGEADLHAYLHSLGDHLRKHPVLVDQFLEDATEIDVDAVSDG
                     QDVYTVIMEQLERAGIHSGDSTCVYPPQTLSPEVIGQVESYTRALGQHLKVVGLMNVQ
                     YAVKEGTVYLLEVNTRASRTVPFASKATGLPLARLATQVIMGTPLSELVLDVSRKNDR
                     VSVKVVVLPFKKFPTLVPVLGPEMQSTGETMGTGEDFATALRKARLGAGYSLEAAAEV
                     APNSKTIAGH"
BASE COUNT          507 a          767 c          877 g          460 t
ORIGIN      
        1 gtgtcggtat cggacctgtg gcaggcgagg gactttgccg gcagacaagg cttccccctg
       61 gtggcgcggg ctgcatactg cctgggcggc acaggctccg ggcacgccaa tgattacgcg
      121 gagcttgagt cgctcattac cacggggttg caggagagcc cggttagctc ggtgttgctg
      181 gagcgtagcg ttaccggctg ggctgagatc gaatacgagg tgctcaggga cgcagtggat
      241 aacgccatca tcgtgtgcaa catggagaac atggacccga tgggcatcca taccggcgag
      301 tcgattgtgg tagcgcccag ccagacgctg tcggacgagg actaccagag gctgcgcacc
      361 gcttcgctgg agatcgtgcg gtcgctgggg gtggtgggcg gctgcaactg ccaattcgct
      421 ctcaaccagc gcacgggcga gttcgccatc atcgaggtca atccgcgcct gtctcgctcc
      481 tcggccctgg cctcaaaagc caccggctac cctatcgccc gtgtggccgc caagatcgcg
      541 ctcggctaca cgctggcgga gttgcgcaac gagatcacgg gggcgagcgc ctgcgcggaa
      601 cccgccctcg actacgtggt ggtgaagctg ccacgctggc cctttgacaa gttccgcaac
      661 gttgaccggc ggctgggcat gacgatgaag agcacgggcg aagtgatggc cattggccgt
      721 acgttcgagc aggcgttcct caaggcgcta cgcagtctag atgtcggtag ttcctggctg
      781 aaggtaagcc ccacctggac cgaggagaag ctggaggagt gcctggcaca cgccacaccg
      841 gcccgccctt ctgccatcta caacgcgcta gcccgtgggt ggagcgtgga ccgcatctcc
      901 gccctgtcgc gcattcaccc ctggttcgtc acccgtatcg ccgctatgtg cgagatggag
      961 cagcggtaca gggagtgccc tacggacgcg atgctgcttg ccgccaagcg tatgggcttc
     1021 tcagacgatc acctgcttga gcttgagggc agcgcctatc acagcgagca ggagcgactg
     1081 caacttagtc tccaggtaag gcggcggatg agtgacctgg ggatgcggcc cacgtataag
     1141 atggtagata cctgcgcggg agagttcgag gcgagtacgc cctacttcta ctcgacctac
     1201 gagcaggagg acgaggctgg gccgttgccc ggaccgaaag tgatcgtact gggaagcggg
     1261 ccgatacgca tcgggcaggg catcgagttc gactactcgt cggtgcacgc ggtggaggcg
     1321 ttacgggcgg ccggaatcaa ggccatcgtc atcaacaaca accccgaaac ggtgagcacc
     1381 gacttccacc tgagcgaccg cctctacttc gagcctttga cggtagaaga agtgttgaac
     1441 gtagtggagc acgagcggga ggggctgctc ggagttatcg cgcagttcgg cgggcaaact
     1501 gccctaaacc tgataaaccc cctcgtggcg gcgggcgtgc ggatactggg cacttcgccg
     1561 gaatctatca gcgtagccga ggaccgggat aagatggccc aactgtgcga gcgcctgggc
     1621 atcccggcac cgaagtgggc tatcgtccac agccacaccg agcttgagac cttcgtgccg
     1681 cagatcggct tcccggtgct tgtgcgcccc tcctacgtgc tggggggtag gggcatgcgg
     1741 gtggtgcacg gcgaggccga cttgcacgcc tacctgcaca gcctgggcga ccaccttcgc
     1801 aagcaccccg tactggtgga ccagttcctt gaggacgcga ccgagatcga cgtggacgcc
     1861 gtgtctgacg ggcaggacgt gtacaccgtc atcatggagc agttggagcg ggctgggata
     1921 cattcggggg atagcacctg cgtctacccg cctcagacgc tctcgccgga ggtcatcggc
     1981 caggtcgaaa gctacacgcg ggcgttaggg cagcacctca aggtggtggg gctgatgaac
     2041 gtgcagtacg cggtaaagga aggcacggtc tacctgctcg aggtgaacac gcgggcctcg
     2101 cgcacggtgc cgttcgccag caaggccacg gggctgcctc ttgcccgcct tgccacgcag
     2161 gtcattatgg gcacgccgct gtcggagctg gtgctcgacg tatcacgcaa gaacgaccgg
     2221 gtatccgtta aagtggtggt gttgccgttc aagaagttcc cgacgctggt gccggtgctg
     2281 ggaccggaga tgcagagcac cggggagacg atgggcaccg gggaagactt cgcgacggcc
     2341 ctacgcaagg ccaggctggg agcgggctac agccttgagg cggctgctga ggtagccccc
     2401 aactcgaaga ccatcgccgg acactgagcg ctggcctggc acaagtaaga tcctgccagt
     2461 tacgggacga cggcgtatcc cgcttatata cgtaaagtac cccttctgca ggaccaaggc
     2521 ggcctctgtc attctgagcg tagcgaagaa tctaaagaag gtgcagtagg taggctgttg
     2581 aatgatacgt actaagatgc tgacgttcaa c
//
LOCUS       DASRWZ010000220         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_771414, whole genome shotgun sequence.
ACCESSION   DASRWZ010000220 DASRWZ010000000
VERSION     DASRWZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771414"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..526
                     /locus_tag="VFH60_03415"
     CDS             236..526
                     /locus_tag="VFH60_03415"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HET6312860.1"
                     /translation="MKLVLAVVSNQAAEGITDGLLAAGFRATRLASTGGFRREGNTTI
                     MMGVEDDDVEPVLAILRSGVQNKKTPVVLDSNGQPTGRGAVFVLPLQNHFHL"
     gene            583..801
                     /locus_tag="VFH60_03420"
     CDS             583..801
                     /locus_tag="VFH60_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312861.1"
                     /translation="MRVRLTDMAYGGDAVGRDPDSGMAVFAWPGITGEDVEVEVGLRR
                     HNLMRGLVTELHTPSSLRVAPPCPYYGA"
     gene            913..2151
                     /locus_tag="VFH60_03425"
     CDS             913..2151
                     /locus_tag="VFH60_03425"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312862.1"
                     /translation="MRPPVPSPRDFHYRNTSHFAIDGSARKLAYFKRNSHDLIAVEEC
                     PISNEGINAMIPRLNALLAQSAAPEDLQMEPRGIMRVWKIAIRHSQVTGQTLVIFHTR
                     PGGTVGGPVSDNVGPRRDRHGRLQAPDRPDEGPNMDENPEASPVFPMRRRDVKRAVPG
                     LAVVEGQQAQAALTVVEVMDDGTVNLLGETREAASETAEAMAETLTGAYVGDMGQGSA
                     MGRAAPPLGAWMERLGGRNYWVDPHGFFQVNTEAAEAMLAEVVAALPSGLGLVVDAHA
                     GVGTFALALAGHAEKVLGYEMVGPAVASARWNALVSSASNVEFRAGRSETLFRALPDD
                     QRPEAVVLDPPRSGCAPSLLREVARREVPRIVYVSCDPSTLARDIKALSSNYTLTSTR
                     VIDMFPQTYHVESVSVLERT"
BASE COUNT          397 a          750 c          721 g          389 t
ORIGIN      
        1 aacatcccgg tggaggacga gattcttcgc tatgctcaga atgacaaaga ccaccttcgg
       61 tcgctcgtct cctatcgttt gcagttaggc cctattctcc ccaagagagt ccccgtaggg
      121 gactttgcca cttcccagcc cgtggcttca gccacagggt gttccccgtc tgaagtgccg
      181 ccactattga cagcacacgc gcttacatct ataatcgggc actgggggtg ctgcaatgaa
      241 attggtgctc gcggtcgtat ccaaccaggc ggcggaaggt ataacggacg gcttattggc
      301 cgcgggcttc cgcgcgaccc ggctcgcctc caccggcggc ttccggcgcg agggcaacac
      361 caccattatg atgggtgtgg aagacgacga cgtggagccg gtgcttgcca tcttgcggtc
      421 gggcgtgcag aacaagaaga cccccgtcgt cctcgacagc aacgggcagc cgaccggccg
      481 tggcgcggtg ttcgtgctcc ccctccaaaa ccacttccat ctttaggccc tcggcaccgg
      541 cctcctcaac ccctctcctc tcttaccctg aaaggtctca acatgcgcgt taggctgacc
      601 gatatggcgt atggcggcga cgccgtgggc cgcgaccccg attccggcat ggccgtattt
      661 gcctggcccg gcatcacggg tgaggacgta gaggtagagg tcggcttgcg acggcacaac
      721 ctcatgcggg gcctcgttac tgagctgcac accccctcca gcctgcgcgt tgcgccaccc
      781 tgtccctatt acggcgcgtg aggcgggtgc cagtggcagc atatcgggta tgacggccag
      841 gtagggttca agcaccacat ccttcgctcg cagcttaccc ggaccggcgg tatagccgac
      901 cccgatgctg taatgcggcc tcccgtgccc tccccgcgtg acttccacta ccgcaacacc
      961 tcgcacttcg cgattgacgg ctcagcacgc aagctcgcct atttcaagcg caacagccac
     1021 gacctcatcg cggtcgaaga gtgccccatc tccaacgagg gcatcaacgc catgatcccg
     1081 cgcctcaacg cgctgctggc ccaaagcgcc gcccctgagg acttgcaaat ggagcctcgc
     1141 ggcataatgc gggtgtggaa gatagccatc aggcacagcc aggttaccgg gcagacgctg
     1201 gtgatattcc atacccggcc cggaggcaca gttggcgggc ctgtcagcga caacgtgggg
     1261 cctcggcggg acaggcacgg ccggttgcag gcccccgacc gccctgacga ggggccgaac
     1321 atggacgaga acccggaagc cagcccggtc ttccccatgc ggcggcgtga cgtgaagcgc
     1381 gccgtgccgg ggcttgccgt ggtggagggg cagcaggcgc aggcggccct caccgtggtg
     1441 gaggtcatgg acgacggcac ggtgaacctg ctcggtgaga cgcgggaagc ggcctccgag
     1501 accgcggagg cgatggccga gacccttacg ggggcctacg tgggcgacat ggggcagggc
     1561 agcgccatgg gccgggcagc gcctcccctg ggcgcgtgga tggagcgtct cggcgggagg
     1621 aactactggg tagaccccca cggcttcttc caggtgaaca cagaggccgc cgaggccatg
     1681 ctggcagagg tcgtggcggc gctgccttcc gggctggggc tggtggttga cgctcatgcg
     1741 ggggtaggca ccttcgcgtt ggcgctggcg gggcatgccg agaaggtgct gggctacgag
     1801 atggtcggcc ctgccgtggc gagcgcccgc tggaacgccc tggtaagctc cgcctccaac
     1861 gtcgagttcc gcgccgggcg ttcggagacc ctgttccgcg ccctccccga cgaccagcgc
     1921 cccgaggccg tcgtgctgga cccccccagg tcgggctgcg cgccctcgct cctgcgcgaa
     1981 gtggcccggc gcgaggtgcc ccgcatcgtg tacgtgtcgt gcgaccccag cacgcttgcg
     2041 cgggacatca aggccctctc ctcgaactac actctcacat ccacccgcgt gatagatatg
     2101 ttcccgcaga cctaccacgt agagtcggtg tcggtactcg aacgcacata gcctgctgcc
     2161 cttcaattca ggtaatattc agcctactgt aagaacctaa cctcccactt aagagatgtg
     2221 ctgtgggagg ttaggttgag gacgactaga gaaacac
//
LOCUS       DASRWZ010000221         4111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_840306, whole genome shotgun sequence.
ACCESSION   DASRWZ010000221 DASRWZ010000000
VERSION     DASRWZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840306"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1296
                     /locus_tag="VFH60_03430"
     CDS             91..1296
                     /locus_tag="VFH60_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET6312863.1"
                     /translation="MADEQPLTFLCITSYEKGHAFLRECKRQGCYVVLLTVDRLRDAP
                     WPHDSIDEFHTIPDLFIREEAIRAASHIARRRYLDRIVPLDDYDVFMAAHLREHFRVP
                     GMGDTTARYFRDKLAMRMQALEKGILVPPFVPVINYDRLREYMDRVPPPWVLKPRAEA
                     ASIGIKRIYGSEELWRALDELGDRQSYFLLEKYILGDVYHVDAITYEREVLFAEVHKY
                     GAPPLNVMHEGGLFVTRTLPRESPEATQVRDLHDRVLGLLGFVRGVTHTEFIRGREDG
                     EYYFLETAARVGGAHITDLVHATTGIDLWAEWAKVEIAGGDRPYAVPAYWKDYGGMIV
                     CLARQEWPDTGAYDDREVVMRINHRHHAGLVVVSPDAARVQALLDNYQPRFYNDFFAN
                     LPAPDRPPT"
     gene            1379..1957
                     /locus_tag="VFH60_03435"
     CDS             1379..1957
                     /locus_tag="VFH60_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312864.1"
                     /translation="MSTSLPPSDVDLAGELRVGERVLWRGRPEREAFVRRTWPLSVFG
                     GALLVAVVAFYMVVLTTEAPNLLAFWGIPFAIAALYMLVGHFILTHREWSHTEYLVTD
                     SRVLIRHGIFSPRLTSYSLVSLPHTQVVMHGDNIGNVMFKGPFGQGYGPPPGYRTMWP
                     YTPGYVLGLMYVRDPGAVQAIIEAARRGESRK"
     gene            2009..3145
                     /locus_tag="VFH60_03440"
     CDS             2009..3145
                     /locus_tag="VFH60_03440"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HET6312865.1"
                     /translation="MRSFTWTQVCGRRLARQRLLEPAPRSELADVVSAVCGIQAQVIT
                     AAELAIGVRVAGIDQQDVRDEIWERRRLVKTYGPRGTLHLLPSGELSLWMAAMRARES
                     LSEPKWYEAAGLEPEQTEALLAGIAEALDGCCLTREELAAEVARRAGPWAKERLASTW
                     GEMLAPAAYTGRLCFGPSQGSRVTFVRADQWAGTWTDLDPEEALIEVSRRYLAAYSPA
                     THQDFARWFWLKPAEARRVMESLAAELEEVEVDGQRAWVLASGADLHPPSGEGSVFLL
                     PQYDCYVLGAVPRDRVVPEASRKRIAGHGRGRFEGAVGLPVLLVDGVVAGMWERHTRS
                     KRTELKVESFVQLTPVQREHLDGEAARVGRFFGTEVSLTLGPLG"
     gene            3202..>4111
                     /locus_tag="VFH60_03445"
     CDS             3202..>4111
                     /locus_tag="VFH60_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312866.1"
                     /translation="MPKLRNVFGTPLAAAVALALVVGLVPSGQALAQEQKGCQAFQET
                     GMTTCGKFLEYWKSHGGLEQQGFPISQQMQEKSDVDGKTYTVQYFERAVFELHLENKP
                     PHDVLLSLLGSMRYKEKDPSGARELPDDAKAAAGMTFPETGKTIKGAFLQYWKDHGGL
                     AQQGFPITNLVRETSELDGKEYTMQYFERAVFELHPENKPPYNVLLSQLGAMRYKMKY
                     AGTTTPPGATTLPEGNWGGEHLAINVSAAGMFIEFDCAHASFKGPISVVNGTFEAPGT
                     FVRETGVQMDPEMAPPAQPAVINGRVN"
BASE COUNT          751 a         1271 c         1366 g          723 t
ORIGIN      
        1 acgtagcgtg ctatcctctc cctataatgc gggcagagtt gccgcgtggc cgggtttgtg
       61 ggtcgcttgg gtagaaaggg gccgcgagaa atggctgatg agcaaccgct gactttcctc
      121 tgcatcacga gttacgagaa ggggcacgct ttcctgcgcg agtgcaagcg ccagggctgc
      181 tacgtggtcc tgctcacggt ggacaggctg cgcgacgcac cctggcccca cgacagcata
      241 gacgagttcc acaccatccc cgacctgttc atccgcgagg aggccatacg cgccgccagc
      301 cacatcgccc gccgccgcta cctcgaccgc atcgtgcccc tcgacgacta cgacgtgttc
      361 atggccgccc acctgcgcga gcacttccgc gtgccgggca tgggcgacac cacggcccgc
      421 tacttccgcg acaagctggc gatgcgcatg caggcgctgg agaagggcat actcgtgccg
      481 cccttcgtgc cggtcatcaa ctacgaccgg ctgcgcgagt atatggaccg tgtgccgccg
      541 ccctgggtgc tcaagccccg cgccgaagcc gcctccatcg ggataaagcg catctacggc
      601 agcgaggagc tttggcgggc gctcgatgag ctgggcgaca ggcagtcgta cttcctgctg
      661 gaaaagtaca tcctgggcga cgtgtaccac gttgacgcga ttacgtacga gcgcgaggtg
      721 ctgttcgccg aggtgcacaa gtacggcgcg cccccgctca acgtgatgca cgagggcggc
      781 ctgttcgtca cgcgcacgct gccccgcgaa agtcccgaag cgacgcaggt gcgcgacctg
      841 cacgacaggg tgctgggcct gctcggattc gtgcgcggtg tgacccacac tgaattcatc
      901 cgggggcggg aggacgggga gtattacttc ctggaaacgg cggcccgcgt cggtggggcg
      961 cacatcaccg acctggtgca cgccaccacg ggcatcgacc tctgggcgga gtgggccaag
     1021 gtggagatag cgggcggcga ccggccctac gccgtaccgg cctactggaa ggactacggc
     1081 ggcatgatcg tctgcctcgc ccgccaggag tggcccgaca ccggggccta cgacgaccgt
     1141 gaggtagtta tgaggataaa ccaccgccac cacgcgggcc tggtggtggt ctctcccgac
     1201 gctgcccgcg tgcaggccct cctcgacaac taccagcccc gcttctacaa cgacttcttc
     1261 gccaacctac ccgcccccga cagaccaccc acttagggaa ttttggattt tagattttgg
     1321 attttggatt gttaggagtt cttctagatc caaaatctaa aattcaaaat ccgtaactat
     1381 gtccacttct ctacctccca gtgatgttga ccttgcaggc gaactgcggg taggggagcg
     1441 ggtgttgtgg cgggggaggc cggagcggga ggcgttcgtg cggcgcacct ggccgttgag
     1501 cgtgttcggc ggggcgttac tggtggcggt ggtcgcgttc tacatggtgg tgctcaccac
     1561 cgaagccccc aacctgctgg ccttttgggg catccccttc gctattgcgg ccctctacat
     1621 gctggtgggc cacttcatcc tcactcaccg cgagtggagc cacaccgagt acctcgtcac
     1681 cgacagccgc gtgctaatcc ggcacggcat cttctcgccc aggctcacga gctacagcct
     1741 ggtcagtttg ccgcatacgc aggtggtcat gcacggcgac aatataggca acgtcatgtt
     1801 caaggggccg ttcggccagg gctacggccc ccctcccggc taccggacga tgtggcccta
     1861 cacccccggc tacgtgctcg gactcatgta cgtgcgcgac cccggtgccg tgcaggcgat
     1921 aatagaggcg gcgcgtaggg gtgaatccag gaaatgagct cgggcacagg attgtgtcga
     1981 tgttggcgat aaaggaggtc tgtcaggcat gaggtcgttc acatggaccc aggtgtgcgg
     2041 gcggcggctc gcacggcaac gcctgcttga gcctgcgccc aggtccgagc ttgcggatgt
     2101 ggtcagcgcg gtttgcggca tccaggccca ggttatcacg gctgccgagc ttgccattgg
     2161 cgtcagggtc gcagggatag accagcagga cgtacgggac gaaatctggg agcgacgacg
     2221 cctggtcaag acctatggcc cgcgtgggac tttgcacctg ctccccagcg gagagttgtc
     2281 gttgtggatg gcggcgatgc gggcgaggga gtcgctgagt gagccgaaat ggtacgaggc
     2341 tgcgggcctc gagccggagc agactgaggc cctattggca ggcatagcgg aagccctcga
     2401 tgggtgctgc ctgacacgcg aagagcttgc cgccgaggtg gcgcgccgcg caggcccctg
     2461 ggctaaggag cggctcgctt ccacctgggg cgagatgctg gcaccggccg cctacaccgg
     2521 gcggctctgc ttcggcccca gccagggtag cagggtgaca ttcgtgcgag cggaccagtg
     2581 ggcgggcacc tggacggacc tcgacccgga ggaggcgcta atcgaggtgt cgcgacgcta
     2641 tcttgccgcc tatagtccag ccacgcacca ggacttcgcc cgctggttct ggttgaagcc
     2701 cgcggaggcc cggcgggtga tggagtcgct tgccgccgaa ttggaggaag tcgaggtgga
     2761 cggccaacgt gcctgggtgt tggcaagcgg tgcggacctc cacccgccct ccggggaggg
     2821 atcggtattc ctgctaccgc aatacgactg ttacgtgctg ggggcggtcc cacgcgaccg
     2881 cgttgttccc gaagcctcgc ggaaacgcat cgcggggcac gggcgggggc gcttcgaggg
     2941 cgcggttggc ctacccgtgc tgctggttga tggggtcgtt gccgggatgt gggagcgaca
     3001 cacgcgaagc aagcgcaccg agctaaaagt agagtctttc gtgcagctta cccccgttca
     3061 gcgcgaacat ttggacggcg aagctgcccg cgttgggcga ttttttggca ccgaagtgtc
     3121 acttacgctt gggccgcttg ggtagggcag ggaactttct ctccctattg ccgttataat
     3181 agacacagat aggaggtgct tatgcctaaa ttgcgtaatg tgttcgggac gcccctggca
     3241 gcggcggttg cgttggccct ggttgtgggc ttggtgccct cggggcaggc cctcgcccag
     3301 gagcagaagg gctgccaggc gtttcaggag accggcatga cgacctgtgg caagttcctg
     3361 gagtactgga agagccacgg cggcctggag cagcagggct ttcccatcag ccagcagatg
     3421 caggagaagt ccgacgtaga cggcaagacc tacacggtgc agtatttcga gcgggccgtg
     3481 ttcgagttgc acctggaaaa caagcctccg cacgacgtgc tgctctccct cttgggtagc
     3541 atgcgctaca aagagaagga ccccagcggc gcgcgggaac tgccggacga tgccaaagcc
     3601 gcggcgggca tgaccttccc ggagaccggc aagacgataa agggcgcctt cctgcaatac
     3661 tggaaggacc acggcgggct ggcgcagcag ggcttcccca tcacgaacct cgtacgcgag
     3721 acgagcgagc tggacggcaa agagtacacg atgcagtact tcgagcgggc cgtgttcgag
     3781 ctacacccgg agaacaagcc gccctataac gtgctgctat cccagttggg cgcgatgagg
     3841 tacaagatga agtacgcggg cactaccacg ccccccggcg ctactaccct gcccgaaggc
     3901 aattggggcg gcgagcacct ggcgattaac gtgagcgcag ccgggatgtt catagagttc
     3961 gactgcgccc acgccagctt caaaggcccc atctccgtcg tcaacggaac attcgaggcc
     4021 cccggcacct tcgtcaggga gaccggcgtt cagatggacc ccgaaatggc acccccagcc
     4081 caacccgccg tcatcaacgg tagggtcaac g
//
LOCUS       DASRWZ010000222         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_291928, whole genome shotgun sequence.
ACCESSION   DASRWZ010000222 DASRWZ010000000
VERSION     DASRWZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291928"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..>2859
                     /locus_tag="VFH60_03450"
     CDS             234..>2859
                     /locus_tag="VFH60_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6312867.1"
                     /translation="MEQASDGIAIYDHQGNIVEANTRACEMLGYSHEELLTRTLVDLT
                     DPEDLAATPIRWAQLRSGQIVLSERVLRHKDGTRIACEISARLLDDGAYVQAIVRDVG
                     ERKQAEMALRESESRFRTVVECLGEGLIITDLDDVVLYANRQLAELSGYSVEEMLGRP
                     AYEIFLHPDKWPMMGQRNNNRKQGVSEQYELEMLRKDGQTIVVQINASPLLDAGGNVV
                     GTLGAQTDITERKRAEETLRRRNDELALLYETTLGLINRLDINSLLEGILTRAADFMG
                     APHGCIYVIEPHSDTMSAQIGTGMFASIVGFQLRKGEGLSGRVWETGQPMSVDDYHTW
                     SGAVPGFDYMRSVVGVPLKSGETVTGVLCLTFVDESHRFRPEDIDLLSRFAQLASLAL
                     ENAKLYAAAQNEIAERTRAEEALRASESRFRAVFEDAIDSIFVMDSKGGFVDVNPAAC
                     QLAARSEEELTGMNILDLVPPSRIGDIEQLTRIFSEHSAVKGEFQIMRPGGDVIDVEF
                     TSKANFLPGYHLAVFSDITEQKLLREQLSHQAFHDALTDLPNRTLFMDRLSQALARST
                     RHQSSLAVIFLDLDDFKVINDSLGHRAGDALLIQVGKRLQNCVRLGDSVARLGGDEFT
                     VLLEDTSIEDATATAQRIAAQLSAPFYVEGCEVFVTSSTGIALSTAGHDGPDDLLRNA
                     DVAMYEAKNKGKNRYTVFDPSMNARAWERLQMEIDLRRALERNELTLYYQLVVDLASG
                     TVRELEALVRWMHPQRGIVSPGDFIPIAEETGLILPIGQWVLEEACRQVKRWQDALPH
                     GQTLRISVNLSPKQFKHSALADDIVRALRQTGLDPRSLQPELTETAAMEDAEATIATL
                     QQLKALGIQLAIDDFGTGY"
BASE COUNT          582 a          875 c          915 g          487 t
ORIGIN      
        1 aagcattaga tttcgaaatt agaatttcgg atttcgaatt tgtttggata ccaactaaat
       61 ttgaaatccg aaatccgaaa tccaaagtag tagcccatat taaagccccc attaggacca
      121 atgggggctt ttccgtgcgg cttctgatgg ggtactctag tggtatgatg gcttctcgga
      181 acaagtccgg cgcggcctcc ggtcgcgcta gtgagagccg ttatcgcctg cttatggaac
      241 aggcttctga cggcatcgca atttacgacc accagggcaa tatagtggag gcgaacacgc
      301 gcgcttgcga gatgctcggc tattcccacg aggagctact cacccgcacc ctggttgacc
      361 tgacagaccc cgaggacctg gccgccaccc ccattcgctg ggcgcaactc aggagcgggc
      421 agatcgtgct cagcgagcgc gtgctaaggc acaaggacgg caccaggata gcctgcgaga
      481 taagcgcccg gctgctggac gatggggcct acgtgcaggc tatcgtgcgc gacgtgggcg
      541 agcgcaagca ggccgagatg gccctgcggg agtcggagtc ccgcttccgc acggtggtgg
      601 aatgcctggg cgaaggcctc atcatcaccg acctggacga cgtggtgctg tacgctaacc
      661 ggcagctagc cgaactttcc ggctacagcg tcgaggagat gctggggcgg cccgcgtacg
      721 agatattcct tcaccctgac aagtggccca tgatggggca gcgcaacaac aatcgcaagc
      781 aaggcgtctc cgagcagtac gagctggaga tgttgcggaa agacggccag actatagtgg
      841 tacagataaa cgcctcccca ctgctggacg ccggcggcaa cgttgtcggc acgctcggcg
      901 cgcagaccga cattaccgag cgcaagaggg ccgaagagac cctgcgccgc cgcaacgacg
      961 agctggccct cctctacgag acgacgctgg gcctcatcaa tcgcctcgac atcaacagcc
     1021 tgctggaagg catccttacc agggccgccg acttcatggg tgccccgcac ggctgcatct
     1081 acgtgatcga accccactcg gatactatgt cggcgcagat tggcaccggc atgttcgcct
     1141 ccattgtggg cttccagttg cgcaagggag aggggctttc ggggcgagtc tgggagacgg
     1201 ggcagccgat gtcggtggac gactaccaca cctggtcggg cgcggtaccg ggcttcgact
     1261 atatgcgctc cgtggttggc gtgcccctga agtcgggcga gacggtcacg ggcgtgctgt
     1321 gcctgacgtt cgtggatgag agccacaggt tcaggcctga agacatagac ctgctcagcc
     1381 ggttcgcgca gcttgcctct ctcgcgctgg aaaacgccaa gctgtacgcc gctgcccaga
     1441 acgagatagc cgagcgcact cgtgccgaag aagccctccg tgcgagcgag tcgcgcttcc
     1501 gtgccgtgtt cgaagacgct attgactcga tattcgtgat ggactccaag ggcgggttcg
     1561 tggacgtgaa cccggcggcc tgccagttgg cggcacgctc cgaggaagag cttaccggca
     1621 tgaacatcct cgacctggtg ccgccgagcc gcatagggga tatcgagcag cttacgcgaa
     1681 tatttagcga gcacagcgcg gtgaagggcg agttccagat aatgcggcca gggggcgacg
     1741 tcattgacgt ggagttcacg tccaaggcca acttcctgcc gggctaccac ctcgcggtgt
     1801 tcagcgacat taccgagcag aagctgttgc gtgagcagct atcgcaccag gcgttccacg
     1861 acgcgctgac cgacctgccc aaccgcaccc tgttcatgga ccgcctgtcg caagccctgg
     1921 cgcgcagcac gcgccaccag tcgtccctcg cggtaatctt cctcgacctg gacgacttca
     1981 aggtaatcaa cgacagcctc gggcacaggg cgggagacgc gctgctgata caggtgggca
     2041 agcggctgca aaactgcgtg cgcctgggtg acagcgtagc ccggctgggc ggcgacgagt
     2101 tcacggtgct gctggaagac acctcgattg aggatgccac ggccacggcc cagcgcatcg
     2161 cggcgcagct ctcggctccg ttctacgtgg aggggtgcga ggtatttgtc acgtcgagca
     2221 cgggcatcgc gctcagcacc gccgggcacg acggccccga cgacctgctg cgcaacgccg
     2281 acgtggctat gtacgaggcc aagaacaagg gcaagaacag gtataccgtg ttcgacccca
     2341 gcatgaacgc ccgggcctgg gagcgcctgc aaatggagat cgacctgcgc cgcgcgctgg
     2401 agcgcaacga gcttaccctc tattaccagc ttgtggtgga cctcgccagc ggcacggtgc
     2461 gggagcttga ggcgctcgtc aggtggatgc acccgcagcg gggcatcgtg tcgccgggcg
     2521 acttcatccc gatagccgag gagacgggcc tcatcctgcc gataggccag tgggtgctcg
     2581 aagaggcgtg caggcaggta aagcggtggc aggacgcgct accccacggc cagacgctac
     2641 gcataagcgt caatctctcg cccaagcagt tcaagcacag cgcgctggcc gacgacatag
     2701 tccgagcgtt gcggcagacc ggcctcgacc cccggagtct gcagccggag ctcaccgaga
     2761 cggcggctat ggaagacgca gaagccacga tagcgacttt gcagcagcta aaggcgctcg
     2821 gcattcaact ggcgattgac gacttcggca ccggctact
//
LOCUS       DASRWZ010000223         8179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_840365, whole genome shotgun sequence.
ACCESSION   DASRWZ010000223 DASRWZ010000000
VERSION     DASRWZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840365"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /gene="folP"
                     /locus_tag="VFH60_03455"
     CDS             complement(<1..1087)
                     /gene="folP"
                     /locus_tag="VFH60_03455"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET6312868.1"
                     /translation="MQSTDNKSYNARIVTLDRPTVASELALIGAQKPGQKILASKAEF
                     LAVKLDNLSCVAANVLKQEMLARGGDCAVHHDCLTLERDTTSVLLTGTREQYDSLVGK
                     LHQQGFDLPEASRQIAALLANLEAQPLPLQLGRYTLPRGQRTLIMGILNVTPDSFSGD
                     SLGDDVEAAVAQARKMHAEGADILDIGGESTRPGADPVPIEEEMRRVLPVIERLNARY
                     GVPLPLSIDTRRSEVARAAVAAGAVIINDITGLRDDPGIADVAAETGAGLVLMHIQGT
                     PRTMQKDPRYDDLLGEVIAYLRAGVDKAVSAGVNRERIWVDPGIGFGKTLDHNLELLR
                     RLGELRSLGCGVLLGTSRKSFIGRVLAH"
     gene            complement(1332..2378)
                     /locus_tag="VFH60_03460"
     CDS             complement(1332..2378)
                     /locus_tag="VFH60_03460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6312869.1"
                     /translation="MTDTLSSIRDWARTLPGLDSGPIDVKFKYERERARSYLLSSGET
                     VMRLRQYIDEPGKKASLLERGARDAAQAEATALELYAVAGLAAQLVWAGELPGDIGGH
                     GVVYRRIEGLSGDKAYLTEAHAERLGRALYEVHSHGANVKLIAPHPRNLAGWWNRVHE
                     QYRDLPPEFLAGLPHALEESLVGLVQSVSGDANAHIRFWQGASLVPVHGSPAMRNVIM
                     EDRRVTLVDWSRFGLGDPAFELAGAVWEMALSGGEALVEHLAGPYLRLAGDIMLERRV
                     MIYRRLLPFGRFLELLHLRWRGEVLTAEQEKQGTMLLSAAMTVYGWPHQTVSETLAQF
                     TNWLKSQPEKPTSM"
     gene            complement(2406..2966)
                     /gene="folE"
                     /locus_tag="VFH60_03465"
     CDS             complement(2406..2966)
                     /gene="folE"
                     /locus_tag="VFH60_03465"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET6312870.1"
                     /translation="MDRDKIQAAVRMILEAVGEDPEREGLQGTPRRVADMYEEVFAGL
                     TIDPSEYLAVGFEESHKEMVVLRDIPFTSVCEHHLLPFVGKAHVGYIPAGRIVGLSKL
                     ARVVEGFARRPQLQERLTSQIADTIVDAIKPVGVGVVIEAQHYCMIMRGVKKPGSTMI
                     TSAMRGLFRNNPPTRAEFLEFVRNNR"
     gene            complement(3059..5533)
                     /locus_tag="VFH60_03470"
     CDS             complement(3059..5533)
                     /locus_tag="VFH60_03470"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET6312871.1"
                     /translation="MIADAHGGIESHRAADDRRGGRWLERLGPPALFLLLSALFLWRP
                     IVTGQVFLPTDLSYKYDYVWIEHELPPGAKIDQNHVVADVADYFYPSVTYAIQRLRSG
                     SFPLWNPHVLTGTPFFAAGQTSVLDPVNLIAFVAGPYDFWTWAAWLRLAMLGFTTYGF
                     LRALGRNVAAGLAAGVVFMVCGFVVVWLNYAVSWSLVWMPALFWASTRLLQTGRLPWL
                     GLTGLSAGALLLGGHAETQFLCGLVWVAYVLYALFVFKHTGRDRVRSLGQVTGAGALG
                     LGIGAVQFVPTVDFMLSSGALGARSGHIQSFDLGETGLRFISQLFPNWTGTPISDNYW
                     VRPELTNFNEQTGYIGLLTLGLGALGAAAMWRRDRLVPFFVVASVLALLLAIRAPGFH
                     LVKALPVLSAGHGVRWLIVFAFFGAVLAGYGVEAMMKQAPGTRRLRDSGLWLASGALA
                     AFALVLAIYLGIRDLHWDRAWGPMLSHVDMARLLYPAQIANNWPIIFLGLGALVLLAR
                     WRGWLPAAAVPVLLVALLYADLWTFGNAYNPTTPHAAILPPTQVTRHLTEHLGHERMA
                     GTLNVLRPNVAAVFGLRDLRGYEPIVEGTFAHMYDDFYHQSRSRVFDLQRKQDLELTA
                     TDQRQLNIAGVRYVATVRKPRVDGSARPYTFILQDGKVALYENLEAFPRAYVVFGSSV
                     VPDTEEAIAAVRSPGLDPNRTVILDAGDAVTAPAALDRSSAPVAWLADEPERVELRAT
                     LPQPGWLVLSDNFAPGWEVEVDGQPATLLRGNVAFRAVAVPAGTHTVSFHYRPRIFYI
                     AAAISTLSTLVALTIGSIYLLRTRRH"
     gene            5823..6917
                     /gene="apbC"
                     /locus_tag="VFH60_03475"
     CDS             5823..6917
                     /gene="apbC"
                     /locus_tag="VFH60_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HET6312872.1"
                     /translation="MPVTKDQVLQALSVVKDPDLGRDIVSLGFIEELNIDDRNNVSFN
                     VVLTTPACPVKEQLKQQSESVVSAIPGVRDVSANMTSRVRQGIMTERQGIPGVRNIVA
                     VGSGKGGVGKSTVAVNLALALAKDGARVGLLDADVYGPNVPLMMGSSAKPQQVGEKIA
                     PVEAYGIKIISMGFFIDADSPVIWRGPMLTKLLTQFMYDVAWGELDYLVMDMPPGTGD
                     VQLTTAQSVPLTGAVMVSTPQDVALLDASKALMMFKKLNVPVLGIVENMSTFVCPHCQ
                     NPTDLFGHGGAQAAAARYSVPFLGEIPLHLRIREGGDAGRPVVVDAPNTPEAEAFMQV
                     ARNLAAQVSVVAYRQQEAKRGAPVAFFSKR"
     gene            7197..>8179
                     /locus_tag="VFH60_03480"
     CDS             7197..>8179
                     /locus_tag="VFH60_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6312873.1"
                     /translation="MQRQEFEASLAPRQGDLLSLPFDQYGRMRIAQNVLTTLLETFAD
                     VDSGNPTASDTLSGISQDPNESKVQNPKSRILDVGGYPGVLHHFVSPVYFDVHVLDVV
                     PDDGSIPNYTQGSGMDLPYPDHSFDVVTALDTLEHIPNTQREQFLTEMMRVARHACLL
                     VNPVQSVEADVAEETLDEYIRWILDAQQEQLREHRDFGLPDASATSDAFERNGWRTHS
                     FATANVYNWLLMMIAKHYLISLRDDKAAGFERTLDRFYNLTFSDADRSLPAYRGVLVA
                     VRPGLEPALERIQVAYPPVATTDTSNTVRLQLTQVLMTLLDLKTANHEDRLL"
BASE COUNT         1552 a         2651 c         2467 g         1509 t
ORIGIN      
        1 cgtgagccag cacccgcccg atgaagctct tgcgagaggt gcccagcaag actccgcagc
       61 cgagagagcg caactcaccc aggcggcgta gcaattcgag gttgtggtcc agcgtcttgc
      121 cgaagccgat gcctggatcg acccatatcc tctctctgtt taccccagcc gacacggctt
      181 tgtcaactcc cgcgcgcaaa tacgctatca cctcgcctaa caggtcatcg tagcgcgggt
      241 ccttctgcat ggtgcgcggg gtgccctgga tgtgcatgag caccagcccg gcaccagttt
      301 ccgccgccac gtcagctatg ccggggtcgt cgcgcagccc cgttatgtcg tttatgatga
      361 ccgcacccgc tgccaccgcg gcccttgcca cctccgaccg gcgggtgtcg attgaaaggg
      421 gaaggggcac accgtacctg gcgttgaggc gttctatcac ggggagcacg cggcgcatct
      481 cttcctcgat aggcaccggg tccgcgccgg gccgggtgct ctcgcctccg atgtcgagga
      541 tgtcggcacc ctccgcgtgc atcttgcggg cctgggccac cgcggcctct acgtcgtcgc
      601 ccaacgagtc gccggaaaag gagtcgggcg tgacgttcag gatgcccatg atgagcgtgc
      661 gctggcccct cggcaatgta taccggccga gttgcagcgg gaggggttgc gcttccaaat
      721 tggcaagcag cgccgctatt tgcctgctcg cctccggcag gtcgaagcct tgctggtgga
      781 gcttgcctac aaggctgtcg tactgctcgc gagtgccggt aagcagcacg gatgtggtat
      841 cgcgctccag ggtgagacag tcgtggtgca ccgcgcagtc tccccctctt gcaagcatct
      901 cctgcttcag cacgttcgcc gccacgcaag agaggttgtc cagcttcacg gcgaggaatt
      961 cggccttact ggcgagaatc ttctgccccg gcttttgcgc gcctatgagg gccaactctg
     1021 aggcgacagt cggccggtcc agcgtgacaa tgcgcgcgtt gtagcttttg ttgtctgttg
     1081 actgcatttt gttctcgttt gccaggtgtg attgctgttc ctagcgacct tatacccccc
     1141 ctgtcatcca gagcgaagcg aaggatcagc cccccgttct tgactctctc ttttgatgca
     1201 ccaatgttcc tctagctgcc agcatcctca acgttgaaga ctctcgccta cccacctgat
     1261 ccttcgcttt gctcaggatg acaaagggag gcttggtggc agcttaatta gggacatgcc
     1321 tgccctacgt tttacatgga agtcggtttt tccggctgtg actttagcca atttgtaaat
     1381 tgtgctaacg tctcactaac tgtctggtgg ggccaaccat ataccgtcat cgcggcggac
     1441 agcagcatgg tgccttgctt ctcctgttcg gcggtgagta cctccccgcg ccagcgaaga
     1501 tgtaggagct ctaggaagcg tccgaagggc agcaggcggc ggtagatcat cacccggcgc
     1561 tctaacatga tgtcgcccgc cagacgcagg tatggcccgg caaggtgctc caccagcgcc
     1621 tcgccgcccg acaaggccat ctcccagact gcccccgcta gctcgaaagc cgggtcgccc
     1681 aggccgaagc gcgaccagtc caccagcgtc acccgccggt cctccatgat gacgttgcgc
     1741 atcgcgggac tgccgtgtac gggcaccagg gacgcgccct gccagaacct gatgtgggcg
     1801 ttggcgtcgc ccgacaccga ttgtaccagc cccacgagcg attcttccag ggcatggggc
     1861 aggcccgcca ggaactccgg cggtagatcg cgatactgtt cgtgtacccg gttccaccag
     1921 cctgccaggt tgcgcgggtg cggtgctatc agcttcacat tcgcgccgtg gctatgaacc
     1981 tcatacaggg cgcgcccaag cctctccgcg tgcgcctccg tcaggtaagc cttgtcaccc
     2041 gacagcccct cgatgcgccg gtagaccacc ccgtgcccgc cgatgtcgcc cggcaactcc
     2101 ccagcccaga ccagctgcgc ggccagaccg gctaccgcgt aaagctccaa cgctgtagcc
     2161 tccgcctggg ctgcgtccct ggcgccgcgc tcaagcagcg acgctttctt acctggctcg
     2221 tcgatgtact gccttaggcg catcaccgtc tcgcccgacg atagcaggta agagcgcgcg
     2281 cgctcgcgct cgtacttgaa cttgacatcg atggggcctg aatccagtcc ggggagggta
     2341 cgcgcccagt cccggatgct gctaagggtg tctgtcatgg ttgctctgcg gaccgttcca
     2401 gcgtgctacc tgttgttgcg gacaaactca aggaactcag ccctggtggg gggattgttg
     2461 cggaacaggc cgcgcatggc cgacgtaatc atggtgctgc cgggcttctt cacgccgcgc
     2521 atgatcatgc aatagtgctg cgcttcgatc actacgccca cgcccaccgg cttgatggca
     2581 tccacgatag tgtcggctat ctggcttgta aggcgctcct gcaactgcgg gcggcgggcg
     2641 aaaccttcca ccacccgcgc cagcttcgac agccccacaa tgcgccctgc cgggatatag
     2701 ccgacgtgcg ccttgccaac gaagggcaag aggtggtgct cgcagacgga tgtgaacgga
     2761 atgtcccgaa ggaccaccat ttccttgtgc gactcctcga agcccacggc caggtactcg
     2821 ctggggtcaa tggtgaggcc ggcgaaaacc tcctcgtaca tgtcggccac ccggcgcggg
     2881 gtgccttgca aaccctcacg ctccgggtcc tcgcccaccg cttccaatat catgcgcacg
     2941 gctgcctgta tcttgtcccg gtccaatctt tcctcccgtt tgtgcgtgtg ggtgggccaa
     3001 ccacgagtat aacatatcgg actcgggtgg tcagtggtca gtggtcagtt gttggttgtt
     3061 agtggcgacg ggtgcggagg aggtagattg agcctatggt tagggcgacc agagtggaga
     3121 gggtgctgat ggcggcggcg atgtagaaga tgcggggccg gtagtggaag gagaccgtgt
     3181 gagtcccggc gggcacggcc actgcgcgga aggccacgtt gccccggagg agtgtggcgg
     3241 gttggccgtc aacttcgact tcccacccgg gagcgaagtt gtcgctcagc accagccagc
     3301 cgggttgtgg cagtgtggcc ctaagctcga cgcgctccgg ctcgtctgcc agccaggcaa
     3361 cgggcgcgct gcttctatca agggcggcag gcgcggttac tgcgtctccg gcgtcgagga
     3421 tgaccgtgcg gttggggtcc agccccggcg agcgcacggc ggctatggct tcctccgtat
     3481 cgggcacgac gcttgagccg aacaccacgt aggcgcgggg gaaggcttcc aggttttcgt
     3541 acaaggctac ttttccgtcc tggaggatga aagtgtaggg cctcgcgctg ccgtccaccc
     3601 tgggcttgcg gactgtggcg acataccgca cgcccgcaat gttcaactgg cgctggtcgg
     3661 tcgcggtaag ctcaaggtct tgcttgcgct ggaggtcgaa gaccctggat cgcgactggt
     3721 ggtagaaatc gtcgtacatg tgggcgaatg tgccctccac tataggctcg tagccgcgaa
     3781 ggtcgcgcag gccgaagacc gccgccacgt ttggccgcaa cacgttgaga gtgccggcca
     3841 tgcgttcgtg gcccaggtgc tccgtgaggt ggcgggtcac ctgagtcgga ggcaggatgg
     3901 cggcgtgagg ggtggtaggg ttgtacgcgt tgccgaaggt ccagaggtcg gcgtagagta
     3961 aggccaccag cagcacgggc acggccgccg ccggtagcca cccccgccac cgggcaagca
     4021 gcacgagtgc ccccagtccc aggaagatta tgggccagtt gttagcgatc tgggccgggt
     4081 aaagaagccg cgccatgtct acgtggctga gcattggacc ccaggcgcgg tcccagtgga
     4141 ggtcgcgtat gcccaggtag atggcaagga ctagggcgaa ggcagcaagg gcaccgcttg
     4201 ccagccacaa cccgctatcc ctcagccggc gcgtgcccgg cgcttgcttc atcatcgctt
     4261 ccacgccata gcccgcgagc accgccccaa agaaagcaaa cacgatgagc cagcggacgc
     4321 cgtgccccgc gctcagcacc ggcagggcct tcaccaggtg gaaacccggc gcgcgtatcg
     4381 cgaggagcag ggccagcacc gaagccacca cgaaaaaagg caccaggcgg tcgcgccgcc
     4441 acattgcggc cgccccgagc gcgcccaatc ccagggttag caaacctatg tatcctgtct
     4501 gctcgttgaa gttggtgagt tccgggcgca cccaatagtt gtccgagata ggggtgcccg
     4561 tccagtttgg gaagagttgg gagatgaagc gcaggcccgt ttcgcccagg tcgaaggact
     4621 ggatatggcc ggagcgcgcg ccgagcgccc cgctggaaag catgaagtcc accgtcggca
     4681 cgaactggac tgccccgatg ccgagtccga gcgcaccggc tcctgtgacc tgccccaggc
     4741 tacgcacccg gtcccgcccc gtgtgcttga atacgaacag ggcgtacaga acgtaggcca
     4801 cccataccag cccgcagagg aactgcgtct cggcgtgccc gccgagcagc agcgcgcccg
     4861 ccgacaggcc ggtaagcccc agccacggta gccgtcctgt ttgcaggagg cgagtgctcg
     4921 cccagaagag ggcaggcatc cacaccaaag accagcttac ggcgtagttg agccacacca
     4981 ctacgaaccc gcagaccatg aagaccacgc ccgcagcaag tcccgccgcc acgttcctgc
     5041 cgagcgccct caggaacccg taagtggtga acccaagcat cgcgaggcgc agccaggccg
     5101 cccaggtcca gaagtcgtat ggccccgcaa cgaaggctat cagatttacc ggatcgagca
     5161 ccgacgtttg tcccgccgcg aagaacggcg tgccggtcag gacgtggggg ttccacaggg
     5221 gaaagctgcc ggagcggagg cgctggattg cgtacgtaac agaagggtag aagtagtcgg
     5281 ccacgtccgc gaccacatga ttctggtcga tcttcgcgcc cggcgggagt tcatgctcga
     5341 tccatacgta gtcgtacttg tagctcaggt cggtcggtag gaagacctgt cccgtgacga
     5401 taggccgcca caagaagagg gcgctgagca gcaagaaaag ggcgggcggg ccgagccgtt
     5461 caagccatcg cccgccccgt ctatcgtcag cagccctgtg gctctcgata ccgccgtgtg
     5521 cgtccgcaat catatggcac gtttataggg cacgtgagct tgcatgtcaa ttatctgttc
     5581 ggggcgctgg cacggtcacc gccccgaacg gccctatacg tgaggtcctt gcacgcttcc
     5641 acactcacat atttccgcgg gcgcaaagcc gtacccgacc gattgacata caatcccaaa
     5701 gcaagtacca tagtggtaac gtatactttg cgaaatcctg cacaaagcat cacaaatcca
     5761 accatcttac ctccaaaatc cgaaattcga aatccgagat tccaaatcga aaggacattc
     5821 ccatgcccgt tacaaaagac caggttctgc aggccctgag cgtcgtaaaa gaccccgacc
     5881 tggggcgtga catcgtatcg ctcggcttca tagaagaact caacattgac gaccgcaaca
     5941 atgtgtcctt caacgtggtg ctcaccaccc cggcctgccc ggtcaaggag cagctaaagc
     6001 agcaatccga gtcggtggtc tccgcgatcc ccggcgttag ggacgtaagc gccaacatga
     6061 cctcccgcgt gcggcagggc atcatgactg agcgccaggg cattccagga gtgcgcaaca
     6121 tcgtggccgt aggctccggc aagggcggcg tgggtaagag caccgttgcg gtgaaccttg
     6181 cgcttgctct tgccaaggac ggcgctcgcg tgggcttgct ggacgccgac gtgtacggcc
     6241 ccaacgtgcc cctgatgatg gggtcgtccg ccaagccgca gcaggttggt gagaagatag
     6301 ccccggtcga agcttacggc atcaagatca tctcgatggg cttcttcatc gacgccgaca
     6361 gcccggtcat ctggcgcggc cctatgctca ccaagctgct cacccagttc atgtacgatg
     6421 tagcctgggg cgagcttgac tacctggtga tggacatgcc ccctggcacc ggcgacgtgc
     6481 aactcactac agcgcaatcg gtgccgctca ccggcgcggt gatggtgtcc accccgcagg
     6541 acgtggcgct gctcgacgcg agtaaggcgc tgatgatgtt caagaagctg aacgtgcccg
     6601 tgctgggcat cgtcgagaac atgagcacct tcgtctgccc gcactgccag aaccccaccg
     6661 acctgttcgg acacggtggc gcgcaggctg ccgcggcccg ttacagcgtg cccttcctcg
     6721 gcgaaatccc cctgcacctg cgcatccgag agggtggcga cgctggccgc cccgtagtcg
     6781 tggacgcgcc caacaccccg gaggctgagg cgttcatgca ggtggcccgc aacctcgccg
     6841 cccaggtcag cgtagtggct taccgccagc aagaggccaa gcgcggcgca cccgtcgcct
     6901 tcttcagcaa gcggtaatga ggagacgaaa acgcaaagac gcaaaggcgc aaagaacgcg
     6961 caaagtgtag ttaaagtttt aagaatgtaa aaggaccagg gtacgacatt agtgccctgg
     7021 tccttttgct tgaataacaa tccttaatct ctgcgcgttc tttgcgctct ttgcgtccct
     7081 tcggctgcgc tcagggcatg ctttgcgttt tcgtctctca ggcacgtttc acgtttcgcg
     7141 gttcccgtgt tatactacgc cccagaacca agcctcagat cggagcagtc caggagttgc
     7201 aaagacagga attcgaggcg agcctcgccc cacgacaagg cgacctgctc tccctaccgt
     7261 tcgaccagta tggccgcatg cgcatcgccc agaacgtgct cactaccctg ctggagacct
     7321 tcgctgacgt agattccggc aaccccaccg cgtccgatac cctctccggt atatcccagg
     7381 accccaacga atccaaagtc caaaatccga aatctagaat cctcgacgtc ggcggctacc
     7441 ctggcgtgct tcaccatttc gtcagccccg tctatttcga cgtgcacgtg ctcgacgtgg
     7501 tgcccgatga cggctccata cccaactaca cgcagggttc cggtatggac ctgccttacc
     7561 cagaccactc gttcgacgtc gtcaccgccc tcgacacgct ggaacacata ccgaacaccc
     7621 agcgcgagca gttcctgacc gagatgatgc gtgtggctcg ccatgcctgc ttgcttgtaa
     7681 acccggtgca atccgtggaa gccgacgtag ccgaggagac gctcgacgag tacatacgct
     7741 ggatactgga cgcgcagcag gagcagctta gagagcaccg agattttggt ctgcccgatg
     7801 cctcggccac ctcggacgcc ttcgagcgca acggctggcg cacccactcc tttgccacgg
     7861 ctaacgtcta caactggctc ctgatgatga tcgccaagca ctacctgatt tccttgcgcg
     7921 atgacaaggc cgccggtttc gagcgcaccc tggaccgctt ctacaacctg actttctccg
     7981 acgccgaccg ctccctgccg gcctatcgtg gcgtgctggt ggcggtgcgg ccgggcctgg
     8041 aaccggcgtt ggaacgcata caagtcgctt acccccctgt agccaccacc gacacgtcca
     8101 acaccgtgcg cctgcaactc acccaggtgc tcatgacgct gctggacctc aagacggcca
     8161 accacgagga ccgcctgct
//
LOCUS       DASRWZ010000224         6091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1251437, whole genome shotgun sequence.
ACCESSION   DASRWZ010000224 DASRWZ010000000
VERSION     DASRWZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251437"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VFH60_03485"
     CDS             complement(<1..1154)
                     /locus_tag="VFH60_03485"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HET6312874.1"
                     /translation="MDQNRSEGSARVTGSDTGPLPPQGLPSTQGGQPTTYVPPHIPAE
                     IRAQGTTGGNYAGETPSAPPPYPPYPPYAPAPQPPVVAPPATYHDRPAHRTPLLGPLL
                     LISAGVVFLLNNLGVLPWSVWETLGRLWPLILIAIGIDLVIGRRNPLLSMLVVLAVLG
                     AGAAFVVANGDFARPGTLASAPVNIRLDTATSADVRVDLGVGNLSIGSMSGDAKLLAT
                     GNLDYFETHGAPRQDVTESGDRASVELAERAGGWNFGSNWFNGGRSPGWEINLNERVP
                     LDLEVDSGTGNLTLDLEKLQVSTVKVDSGTGNASITLPATANGTIVEIDGGTGNMELF
                     VPEGLEARIEVDSGIGNTTVDPRFSSQGEDTYESSNYDTATNKVTIKIDHG"
     gene            complement(1254..1793)
                     /locus_tag="VFH60_03490"
     CDS             complement(1254..1793)
                     /locus_tag="VFH60_03490"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET6312875.1"
                     /translation="MNGTSRLYRSTSQRMLGGVAAGFASYFGIDPNVVRLIFLVLTFV
                     TGGGFALVYMLLWLLIPGTASTAADVNQVVNENIGDMGSQLRSFTGTKASNGGNGGNG
                     GNGGVAQPNGGPNPAQGQLPPNAASVQPRQGNPAVALIVIGVFFLLLNLGFFRFFAWG
                     VWWPLLLVGLGVMMISRRA"
     gene            complement(1960..2157)
                     /locus_tag="VFH60_03495"
     CDS             complement(1960..2157)
                     /locus_tag="VFH60_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007410826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET6312876.1"
                     /translation="MNYKRLARSNNVMFAGVAAGLAEYFNLDPTLVRVLFVALGIISG
                     FFPALFVYLVLWFIMPRYATQ"
     gene            2887..3963
                     /gene="pheS"
                     /locus_tag="VFH60_03500"
     CDS             2887..3963
                     /gene="pheS"
                     /locus_tag="VFH60_03500"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET6312877.1"
                     /translation="MATLQEQIEGARQEALAALAQVGDSGGLEEWNRQYLGKKGLLTG
                     LFRGVGGLPPEERSAAGQAINTAKGELEQAYVGAEKRIGQGELQRRLVEEAVDITLPG
                     RHMPAGHLHIINQTARDVIQAFRDMGFQVYEGPDIELDMYNFELLNIPPEHPARSMQD
                     TFYVDDRLLGMREPGASMDELPPVVLRTHTSPGQIRLMRRVEPPVRLVIPGRTYRSEA
                     TDATHEAYFTQMEGMAIDKKVTLADLKGTLAAFARAIFGSDRKVRFRCDYFPYVEPGV
                     DLSIDCINCDGSGCRSCKYTGWLEILGAGMIHPQVLINGGVDPDDYSGFAFGVGFERV
                     AMLRHGIDDIRLFYGNDLRFLQQF"
     gene            4056..>6091
                     /gene="pheT"
                     /locus_tag="VFH60_03505"
     CDS             4056..>6091
                     /gene="pheT"
                     /locus_tag="VFH60_03505"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET6312878.1"
                     /translation="MPMRAPLSWLREYIDINVPVEELARRLTLAGQEVEHIVTVGAEW
                     ENIYTGVVARLERHPNADRLNLATVEYGAGKEITVVTGAPNIEQGQKVVLGLVGCKYL
                     DQHVSPPRWSTLKPAKIRGIQTEGMVMSEAELGLSEEHEGIIVLDAQTPLGLTAQEVL
                     GDTILEIDVTPNNGRILSMIGMAREIAALFGGEVRYPSSEWHAAGPPAEQILSVEIQD
                     PDLCARYSGAVIQNVKIGPSPAWMQRRLALAGMRPINNIVDITNYVMLEMGQPLHAFD
                     YEAVEGHKIIVRRARQGEQIETLDHVHRTLDPNMLAICDINKPVALAGVMGGANSEIG
                     TEGTATVLLESAHFSQRTVRRTARLLRLPSEASYRFERWVDPTLTVPAMKRAADLMRQ
                     LGGGTIAEGYADEYPRPLKPTRLHFYTSEAERLLGITLPPSEIAKMLERLEFKVDLPH
                     NADAVGQYTTMLIDVPSYRNDVTLPADIVEEVARMVGYDIIPETLLDGRLPRQEVNYS
                     REIAEEMTDLMIAAGMDEVLTYSVAHSEELRKLAELEDRLEGTSEDGATERPRYRAWD
                     TTRPPVTIVNPTSSRQDVMRPSLLPNMLDTLLMNMRLMPETPIRIFELGKVFMARTEE
                     EVEARRAEIERERVRYPRLRNWQPVQDEERLPLEPRRLMGLMSGPRQPRSLFNQ"
BASE COUNT         1191 a         1681 c         2120 g         1099 t
ORIGIN      
        1 ccatgatcga tcttgatggt aaccttattc gtggcggtgt cgtagttgct cgattcgtag
       61 gtgtcctccc cctggctgga gaagcgaggg tctacggtcg tgttgccgat gccggagtct
      121 acctcgatgc gggcttcgag gccctcgggc acaaatagct ccatgttgcc cgtgccgcca
      181 tctatctcca cgatggtgcc attcgccgtg gcgggcaggg taatgctggc gttgccggtg
      241 cccgaatcga ccttgaccgt gcttacctgc aacttttcca ggtcgagggt caggttgccg
      301 gtgccgctgt ctacttccag gtcgaggggc acgcgctcgt tcaggttgat ttcccaaccg
      361 ggcgagcggc cgccgttgaa ccagttggag ccgaagttcc agccaccggc gcgttcggca
      421 agctccacgc tggccctgtc gccgctctcg gtaacgtcct ggcggggagc gccgtgggtc
      481 tcgaagtagt ccaggttgcc ggtggcgagc agcttagcgt cgccggacat gctgcctatg
      541 ctgaggttgc cgacgcccag gtccacgcgc acgtccgccg aagtggcagt gtcgagccgg
      601 atgttgacgg gggcgctcgc cagcgtgccc ggacgcgcaa agtcaccgtt cgcgaccacg
      661 aaagcggccc cggcccccag cactgcgaga acaaccagca tggacagcag ggggttgcgg
      721 cgaccgatta ccaggtcgat gcctatagcg atgaggatga ggggccagag gcggcccagc
      781 gtctcccaca cactccatgg gagcacgccg aggttgttta gcaggaagac gacaccggcc
      841 gaaataagca ggagcggtcc gagcagcggt gtgcggtgcg ccgggcggtc gtggtaagtg
      901 gcgggcgggg caaccaccgg cggctggggg gcgggtgcgt agggcggata tggtgggtag
      961 ggtggcggag cggagggtgt ttcgcccgcg tagttgccgc cggtggtgcc ctgcgctcgt
     1021 atttcggcgg ggatgtgtgg gggcacgtac gtggtgggct gacccccctg ggtggatggg
     1081 aggccctgcg gcgggagggg gccggtgtcg ctgcccgtca ccctggcgct gccttcgctg
     1141 cgattttgat ccatagaact tcctcctggt atgtaaagca aggatgcagt agtccgtggg
     1201 gagtactgca tcggtagcgg tgtacgctat ttatttagtg cctgccggac gtatcaggcg
     1261 cggcgactga tcatcatcac gcccagaccc accaggagca gcggccacca gactccccag
     1321 gcgaagaagc ggaagaagcc gaggttgagc aacaggaaga atacacctat gactatcagg
     1381 gccaccgcgg ggttgccctg ccgaggctgg acggaagcgg cattcggggg cagttgtcct
     1441 tgagcggggt tcgggccgcc gttgggctgg gccacaccgc cattgccgcc gttgccaccg
     1501 ttgccgccgt tgctagcttt cgtgccggtg aagctgcgca actgggagcc catatcgcct
     1561 atgttctcgt tgacgacctg gttcacgtca gcggcggtgc tggcggtgcc tggaataagc
     1621 agccacagca gcatgtagac cagggcgaac ccgccgccgg taacgaacgt tagcaccagg
     1681 aagatgagtc gcacgacgtt cgggtcgatg ccgaagtagc tcgcgaaccc ggcggccacg
     1741 ccgcccagca tcctctgact agtgctcctg taaagacggc tcgtgccgtt cattctttgt
     1801 ccctccaaaa cgtatttagt gcctgttaag cggaaatcca ggcggcgaga tgttagttgt
     1861 tagttgttag ttgttagagt gctttatcga tcctggtgta ccaggcttcc aacgcagcta
     1921 ctcagtcctc agtcctcagt cctcagtcct catcactcgt tactgggtcg cataccgggg
     1981 catgatgaac cagaggacca ggtagacgaa cagggccggg aagaagccgg agatgatacc
     2041 gagcgctacg aacagcaccc ggaccagggt cgggtcgagg ttgaagtact cggccagtcc
     2101 ggcggcgaca cccgcgaaca tcacgttgtt actgcgcgcc aatcgcttgt aattcacttg
     2161 cgcccctcct tcatttatgc ttgcaacttt acttacgcag ggtcgcggca aaatgtcgca
     2221 aagttggcga acaatttctt agccggatac gaatgccgag gctgctttgc atgcggcctt
     2281 gactgggtag tgaaagtgtc gcgcggtaag tgtacaaccc cggcttgatg aagtgatggt
     2341 gcctccggca tattggagca tcctgcgttc gatggagtgg actcctgaga cttgagatgt
     2401 aggtacccgg tgacgtacga ctcttgacaa ggctgcgcca cttcggttac taatagatgc
     2461 tacaaggccc ggtctcgcag acgggcagca caaatgaaga gcgacaatca aaggcgaaga
     2521 cggagagagt aagtctgcgg cggcgcacag ggaaggacag gccagggact gagagcctac
     2581 ctcgccaaaa cagcaccgaa gttcactccc gagccggcag gggaagttac gaattacgaa
     2641 ttacgaatta cgaattacga attgggctag atagacgaga ctcaattcgt aattcgtaat
     2701 tcgtaattcg taattccaac gcgtagtctg tcccggtagc tcccgttatc gagcacggag
     2761 cggtccgcgt acctggtggg tgcggggccg aaatcagggt ggtaccacgt ggccgcgcct
     2821 cgtccctggc gagcagcgcg gtctttttgt ttaagtcgag attggcaggg gtgaaggagc
     2881 acttgtatgg cgacattgca ggaacaaata gaaggggcca ggcaggaggc gctggcggcg
     2941 ctggcgcagg tgggcgacag cggcgggctt gaggagtgga accggcagta cctgggcaag
     3001 aagggcttgc tgacggggct gttccggggc gtgggcgggc tgccccctga ggagcgttcg
     3061 gcggcggggc aggccatcaa cacggccaag ggcgagttgg agcaggcgta cgtgggggcc
     3121 gagaagcgca tcgggcaagg ggagttgcag cgcaggctgg tggaggaggc ggtggacatc
     3181 acgctgccgg gccggcatat gcccgcggga cacctgcaca tcatcaacca gacggcccgc
     3241 gatgtaatcc aggcgttccg cgacatgggc ttccaggtgt acgaggggcc agacatagag
     3301 cttgatatgt ataacttcga gctgctcaac atcccgcccg agcaccccgc ccgctcgatg
     3361 caggacacgt tctacgtgga cgaccgattg ctgggtatgc gcgagccggg ggcgagcatg
     3421 gacgagttgc cgcccgtagt gttgcggacg catacctcgc cggggcaaat ccggctgatg
     3481 cggcgggtgg agccgccggt gcgcctggtg ataccggggc gcacctaccg ctcggaggcg
     3541 accgatgcca cgcacgaggc gtacttcacg cagatggagg gcatggcgat agacaagaag
     3601 gtgacgctgg ccgacctgaa gggcacgctg gcggctttcg cccgcgctat tttcggcagc
     3661 gaccgcaagg tgcgcttccg ctgcgactac ttcccgtacg tcgagccggg ggtggacctt
     3721 tcgattgact gcatcaactg cgacggcagc gggtgccggt cgtgcaagta cacgggctgg
     3781 ctggaaatac tgggggcggg catgatccac ccgcaggtgc tcatcaatgg cggtgtcgat
     3841 cccgacgact acagcggttt cgctttcggg gtgggcttcg agcgggtggc aatgctacga
     3901 catgggatag acgatatacg gctgttctac gggaacgacc tgagattcct acagcagttc
     3961 taagggttat tgtggagaag tgaaaccgca aagacgcaaa gagcgcaaag gacacgggaa
     4021 gatagttatt gttttgaggt aagaggagag aagctatgcc gatgagagcg ccgctctctt
     4081 ggttgagaga gtacattgat ataaacgtgc cggtggagga gctagcgcgc cgccttacgc
     4141 ttgctgggca ggaggtggag catatagtca ctgtgggtgc ggagtgggag aacatttata
     4201 cgggggtggt tgcgcggttg gagaggcacc ctaatgccga ccggctgaac ctggctaccg
     4261 tggagtacgg ggcgggtaaa gagatcacgg tggtgacggg cgcgcccaat atcgagcagg
     4321 ggcagaaggt ggtgctgggg ctggtggggt gtaagtacct ggaccagcac gtatcgccgc
     4381 cgaggtggtc tacgctgaag cccgccaaga tacggggcat ccagaccgag ggcatggtca
     4441 tgtcggaggc ggagttgggg ctttcggagg agcacgaggg catcattgtg ctcgacgctc
     4501 agacccctct cggcctcacc gcgcaggagg tgctcggcga tacgatactg gagatagacg
     4561 ttacgccgaa caacgggcgc atcctctcga tgataggcat ggcgcgggag atcgcggccc
     4621 tgttcggggg ggaggtgcgc tacccgtcga gcgagtggca cgcggcagga ccgcccgccg
     4681 agcagatttt gagcgtggag atacaggacc ccgacctgtg cgctcgctac tcaggcgcgg
     4741 tgatacagaa cgtgaagatc ggcccctcgc ccgcgtggat gcagcggcgg cttgcgctgg
     4801 cgggcatgcg gcctataaac aatatcgtgg acatcaccaa ctacgtcatg ctggagatgg
     4861 ggcagcctct gcacgccttc gactacgagg cggtggaggg ccacaagatc atcgtgcgcc
     4921 gtgccaggca gggggagcag atcgagacgc tggaccacgt gcaccggacg ctcgacccca
     4981 acatgctggc tatctgcgac atcaacaagc cggtggccct ggcgggggtg atgggcgggg
     5041 ccaacagcga gatagggacg gagggaacgg ccacggtgct gctggagtcg gcgcatttca
     5101 gccagcgcac ggtgcgccgg accgcccgcc tgctcaggct gccttcggag gcgtcgtacc
     5161 ggttcgaacg ttgggtggac cccacgctga cggtgcctgc catgaagcgg gcggccgacc
     5221 tgatgcggca gctaggcggg ggcacaatcg cggagggcta cgcggatgag taccctcggc
     5281 ctctcaagcc gacgcggctc cacttctaca cctcggaggc cgagcgcctg ctgggaataa
     5341 cgctgccacc cagcgagata gcaaagatgt tggagcggtt ggagttcaag gtggacctgc
     5401 cccacaacgc cgacgcggtg ggccagtaca ccaccatgct gattgacgtg ccgtcgtacc
     5461 gcaacgacgt gaccctgccc gccgatattg tggaggaagt ggcgcgtatg gtgggctacg
     5521 atatcatccc ggagacgctg ctcgatggca ggttgccgcg gcaggaggtc aattactcgc
     5581 gggagatcgc ggaggaaatg accgacctga tgatagcggc gggtatggac gaagtgctga
     5641 cttactcggt ggcgcacagc gaggagttgc gcaagctggc ggagttggag gacaggcttg
     5701 agggtacgag cgaagatgga gcgactgaga ggcctcggta ccgtgcctgg gacacgacgc
     5761 gcccgccggt gaccatagtg aaccccacga gtagcaggca agacgtgatg aggcccagcc
     5821 tgctgccgaa catgcttgac acactgctga tgaacatgcg cctgatgccg gagacgccga
     5881 ttcgcatctt cgagcttggc aaggtcttca tggcgcgcac cgaggaagaa gtggaggcgc
     5941 gccgggccga aatcgagcgc gagcgcgtgc gctacccacg cctgcgtaac tggcagccgg
     6001 tgcaggatga agagcgtctg ccgttggagc cacgtcgcct tatgggcctg atgtcggggc
     6061 cgcggcagcc gcgctcgctt ttcaaccagg a
//
LOCUS       DASRWZ010000225         4063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_17899,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000225 DASRWZ010000000
VERSION     DASRWZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17899"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFH60_03510"
     CDS             complement(<1..568)
                     /locus_tag="VFH60_03510"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6312879.1"
                     /translation="MSSGLPANQIEAATQPGGEHAAQGGRLPYLPGLDGLRAIAVTGV
                     LLYHAGMLWLPGGFLGVEVFFVISGYLITSLLLSEWQRHGRVNLLAFWLRRARRLLPA
                     LFLLIVSTLTFAVLFLPEEVAGLRGDAVAATGYVTNWYLIFNQQSYFETMGRPSLLQH
                     LWSLAVEEQFYLLWPLLFVGGIRILRRGL"
     gene            complement(1271..2392)
                     /locus_tag="VFH60_03515"
     CDS             complement(1271..2392)
                     /locus_tag="VFH60_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312880.1"
                     /translation="MKKHITTYLLLAVATVLLVACGAPPIEQGGSSTPISAPTAATAA
                     TASETVPATTPAPTAIITTPPATLAPTAAATASATQPARTEVPDTPATPGSADPGVGS
                     DVVGSNVCKFILGAAVAPEGLAVKPCDWNAPVLCVLSGDELAGQVELFMSHLETLPEF
                     RQTLESEGLTPGSIDYRDPEQAPALAAALRTFVEDYHRTFEEDRRLTYGDKATYTRLE
                     TQEVRVADIPGLRYGFVVHDNGGKVLERWISFAAFDGSILYILTPHYDPRSHMTFRSD
                     EELQRFEPYMSEIATGLKLPFPVLETPVKQVVTLARVPLFRYYGNGSNPVAEVPAGRT
                     LLVSGRSPDDRAWRVECPGDVAGECWVSAHAKLTRPGVP"
     gene            2897..3304
                     /locus_tag="VFH60_03520"
     CDS             2897..3304
                     /locus_tag="VFH60_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312881.1"
                     /translation="MEDIEDTAAVGTYGMELGETASLAVTWQTALQPVAVPPAHRHPA
                     RAYLARLAVGSRPAMRGALAVAAELLTGGRCTWETMPWHLLEAQHTKALRAELAHRYA
                     PTTANKMLAAVRGVLREAWELGLVPGEQYHRAT"
     gene            3314..3913
                     /locus_tag="VFH60_03525"
     CDS             3314..3913
                     /locus_tag="VFH60_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET6312882.1"
                     /translation="MRGERLLRGRALGGGELTALFEVCKSDKSPAGARDAALIAVLYG
                     SGLRRSEAVALDLASYNREEGSLTVRAGKGNKDRMCYTASGQSKLIDAWLTLRGEQAG
                     PLFCAIAKGGKVKERRLTDRAVLYIVQRRARQAGVEHFSPHDLRRTMIGDLLDAGADI
                     STVQKLAGHANVSTTVRYDRRDEVSKKRAARLLNIPGAD"
BASE COUNT          829 a         1211 c         1281 g          742 t
ORIGIN      
        1 ccaggccccg gcgcaggatg cgaatgcccc caacaaagag gaggggccat agcaggtaaa
       61 actgctcctc gaccgccagg gaccacaggt gctgcaagag cgaaggccgg cccatcgtct
      121 cgaaatagga ttgctggttg aatatcaggt accagttggt gacgtagccc gtggcggcca
      181 cggcatcgcc ccgcagcccg gctacctctt cgggcaggaa cagcaccgca aaggtgagcg
      241 tggagacgat taggaggaag agggcgggga ggagcctgcg cgcccgcctg agccagaaag
      301 ccaggaggtt gacgcgcccg tgcctctgcc actcggaaag cagcagcgag gtaatcaggt
      361 agccgctgat gacgaagaag acctctacgc ccaggaaacc gccgggcagc cagagcatac
      421 cggcgtggta gagcagcacc ccggtcacgg cgatggcgcg caggccgtcc aggcccggca
      481 ggtacggtag acgcccgccc tgtgccgcat gctctccgcc cggctgagtt gctgcctcta
      541 tttggttggc tggcagacca cttgacatcc tactttcctg gtgcgacgac ggacgatacc
      601 aaggatagcg cgcttccgta agagaacgtg ccttaccctg tgtagattac agcacaagtc
      661 acgggcgtgc aattagacac acggactaat ctaccctaat gatttgtacc tacctaaggc
      721 cgtgcccgcc aggggcttgc gccccgcgat ataccgggca ggcgcgcgtc acacgtgttg
      781 aggtggaact gctgagtact gctacattat agcgcgctac agcaatgctc tcaatggccg
      841 gatcaatcca agccgccaac tcttcccgct ggcggatttc acatagtggt cgcgaattgc
      901 tgccaactgg caggctctgg cactacattc ggcacgcgct gtacgtacaa accatgctta
      961 agtctcagaa aaacgcacga cgcatttaag taattggcta aaaaactaat ataccaaaaa
     1021 taaaaaccaa cagggactat gtaagagaca tatcccctga gatagtgctg cttgcagtgc
     1081 aatggtacga agggaacgta gggtcgaggt gcaagcccgc tagacagacc cacgcgccgt
     1141 attcgttcca gagctagtac gtcaagtagg gcatgcaccc gcagcgctta tttctctgga
     1201 cgccgcaaag cgacatatcc ccaaaggcac atacattccg gctttgccga gcgcatcaaa
     1261 cctacagtac ctacggaacc ccaggccgtg tcagcttcgc gtgggccgat acccagcact
     1321 cacctgctac gtcgccgggg cattcgaccc gccaggctct gtcgtcgggg ctgcgcccgc
     1381 ttacgaggag ggtccgtccg gcgggcacct cggccaccgg gttcgagccg ttgccgtagt
     1441 acctgaacag cggcacccgc gccagggtca ccacctgctt taccggagtt tccagcaccg
     1501 ggaagggtaa tttcaggccg gtggcaattt cgctcatgta aggctcgaac ctctgcaact
     1561 cttcgtcgga gcggaaagtc atgtgggaac gggggtcgta gtggggcgtg aggatgtaga
     1621 gaatactccc gtcgaacgcc gcgaaggaaa tccaccgttc gagcaccttt ccgccgttat
     1681 catgcaccac gaagccgtac cgcaggccgg ggatgtccgc gacccgcacc tcctgcgtct
     1741 ccagccgcgt gtatgtggcc ttgtcaccat aggtaagccg ccggtcctct tcgaaggtcc
     1801 tgtggtagtc ctccacgaag gtcctgagcg cagcggccag ggcaggtgcc tgctcggggt
     1861 cgcggtaatc tatcgaaccg ggggtgagtc cctcgctctc cagagtttgc cggaattccg
     1921 gtaacgtctc caggtgcgac atgaatagct cgacctggcc cgcaagctca tctcctgaga
     1981 gcacgcagag caccggggcg ttccagtcac agggcttcac cgcgaggccc tccggggcaa
     2041 cggccgcgcc caagatgaat ttgcagacgt tgctgcccac gacatcgcta ccgacgcctg
     2101 ggtctgcgct acccggcgtg gccggtgtgt ccggtacctc ggtcctggcg ggctgagtgg
     2161 cgctggcggt ggcggccgcg gtgggtgcta gcgtggcagg tggggtcgtg ataatggcgg
     2221 ttggcgctgg ggtggtcgcc ggtacagtct cggaggccgt tgctgctgtg gctgctgtgg
     2281 gggctgatat aggcgtgcta ctgccgccct gctcgattgg tggcgctcca catgccacca
     2341 gcaagactgt tgcgacagcc agcaatagat atgtcgtgat gtgtttcttc atttgcaatt
     2401 cctcagatgg cgcgggtgca atgcgtaatg aggtgccccc gcttccctat accttgtacc
     2461 ttacgctgtc catagagctt tcctatgctc ctggcgtcat tttatcagga gcgccgaccc
     2521 ttaccattgg acacaagagg tatcgggcag tactcacaag tatttgcatc agcctcggac
     2581 ttgcggccta gtacggtgcg gatcgatgtg gaaaacgcct tcatgctgcc gcgatgaggg
     2641 gtaaaatcag cccgacacat tacttcctag aatggtcctt ctggaaagta attgcacatt
     2701 ctacagggtg gctgctgagg ggagggggtg cagctgggcc aggtcgagca ctgtccccat
     2761 acctgacatt aagaacgtgc tcatcttcta atgccgagat ttgcaccggc ggacaagcag
     2821 acaagcggca gcttcatacg gcgatggata gaacgttgaa cggtacgcca gtatgaggac
     2881 ggcgaaagga gtagcaatgg aggatataga agacacggcg gcagtcggca cttacggaat
     2941 ggaactgggt gagaccgcca gccttgccgt gacgtggcag accgcgttgc agcctgtggc
     3001 cgtgcctccc gcccaccgcc acccggcaag ggcgtacctg gcgcggttgg ccgtgggatc
     3061 gaggccggcc atgcgggggg cgctcgcggt ggccgccgag ttgcttaccg ggggacggtg
     3121 cacctgggag acgatgccct ggcacctgct ggaggcacag cacacgaaag ccctgcgcgc
     3181 ggagctggct cacaggtacg cccccaccac cgccaacaag atgctggcgg ccgtgcgggg
     3241 agtgctgcgc gaggcctggg agcttggcct tgtgcccggc gagcagtacc acagggccac
     3301 ctagttccgc accttgcgcg gggagcggct gctgcggggc cgcgccctgg gagggggcga
     3361 actgacagcg ctcttcgagg tctgcaagtc ggacaagtcg cctgctgggg cgcgcgatgc
     3421 cgccctcatc gcggtcttgt acggctccgg gctgcggcgt tcggaggcgg tggctctgga
     3481 cctggcttcg tacaacaggg aggaaggcag cctcaccgtg cgggcgggga agggcaacaa
     3541 ggaccgtatg tgctacaccg cctcgggcca gtcgaagcta atcgacgcct ggctcacgtt
     3601 gcgcggcgaa caggcgggtc cgctcttttg cgctatcgcg aaggggggca aggtcaagga
     3661 gcgcagattg acagatcgcg cggtcctcta catcgtgcaa cgccgggcca ggcaggccgg
     3721 ggtggagcat ttttcgcccc acgacctgcg ccgcaccatg attggcgatt tgctggacgc
     3781 gggggccgac atctcgaccg tgcagaagct ggcggggcac gccaacgtat ccactaccgt
     3841 gcgctacgac cgccgggacg aggtcagcaa aaagcgcgcg gccaggttgc tcaacatacc
     3901 cggtgcggac tagaagctaa ttgaaaaaca agatagtcgg acctgcgcac ccaaagcccc
     3961 ttatatgtca tttcgaacgt agtgagaaat ctcgtccttc agcacccaaa tgccaccttt
     4021 ctaatgccac ctttctaaca gcgtagagtc tcgtgagaaa agg
//
LOCUS       DASRWZ010000226         5779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_977148, whole genome shotgun sequence.
ACCESSION   DASRWZ010000226 DASRWZ010000000
VERSION     DASRWZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977148"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..2062)
                     /locus_tag="VFH60_03530"
     CDS             complement(359..2062)
                     /locus_tag="VFH60_03530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET6312883.1"
                     /translation="MAAISRKHRTKRTALLSLLLLVLTLSWSGISAAAPATQEALAEP
                     EISARAAIVVEYPSGRVLYSRAMHDQLAPASTTKILTAIMALEYGKLDEVFTTTDEDL
                     VGESSMGLASGERQTLLDLLYGMMLPSGNDASMVVARGLAEKAAPPETSSEDPIARFS
                     AMMNKRVEQLGLRNSHFANPHGLDMEGHYSSAYDLASLSWYAMHFPTFNEVVKQISYD
                     APGHPLLNTNEMLTRYEGADGIKTGWTDAGGLCLVTSATRGERRLISVVLNSPRWYAD
                     SGALLDFGFAKLAQVPTDAGAEVLEVTRRGVADWILGEGSTPSMLPPQMAGGGGVVAQ
                     PEQAEQSEAVIAAPAPVQEQEAPLQSIAGASSPTTGSNNVPWALLLSGLVGVVIFYAV
                     ATRLAAFRPRRLIASVAAHLPQREQVTYAPPPTPIRVPQRRQVEQAGTPGRPTPAHRT
                     LGTEPNLRRREPNLLVSPEQLYRQHLARAVDLAWEGRQGSSMAEFLLALRMSGSLDVA
                     EIVAVHQLGPDAVLALSRAQVAAGQLEDAHRTLTHGILVLPQERFLRMAIKQLESDYC
                     A"
     gene            complement(2204..2734)
                     /locus_tag="VFH60_03535"
     CDS             complement(2204..2734)
                     /locus_tag="VFH60_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312884.1"
                     /translation="MIYLHGEQDEMEEDLLPANTATGTNLLPGARLRPSQFISQVGSE
                     VMERLGASALEFHVRARSRLVQFWSGDNASVHYEVWVHEREARLEIGFHLEADEAHNA
                     AVYRELDRCLLDVQFQLGGSVWLEPWDKGWVRLYETQPLWPLDPARVEETAERTAAFV
                     QVVQPLYESILERLAE"
     gene            complement(2731..3726)
                     /locus_tag="VFH60_03540"
     CDS             complement(2731..3726)
                     /locus_tag="VFH60_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312885.1"
                     /translation="MGTTLENKLAAPSPDRTDGDRLPLVSSVLGALAYADLFDAPLSA
                     EEIHKYQITTSYSRAEITQALSREPDLLARVATDGTYYCLKGREGLFSVRAERAEASR
                     KVWERARRYAGWLRRLPFVRMVAVTGALAVNNVNGRYDIDLLVISRSGRVWLCRRGLI
                     GWVRVGRLLGDDLCPNYILSEGSLELSQRDFFTAHELAQMVPVYGARVYERMLRANEW
                     ASTYLPALAGMSSLPEMDDRRAPRPGPVERALRGRAFDRWERWELERLRGKLRSQIGR
                     DAEVVLTPDQCKGHTGLHRAAVMTRFMSRLAELGLHDVADALQEAQIRTTTEPDA"
     gene            complement(3831..>5779)
                     /locus_tag="VFH60_03545"
     CDS             complement(3831..>5779)
                     /locus_tag="VFH60_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312886.1"
                     /translation="VRRTAGSLAAVYATLFFAVSPLSAALGQQFSPAALILAMQALAV
                     LALLSWRDTVNHARVQGSTGRFLVAITVGAIAALLDPGSIFLAVPAAYLVLSVDGERL
                     QGRITRRTPRATADTWSDAWHKSAHRGKVLVYSAMLVIAAGGWWLYSSKVDTLVLGSG
                     DGAGSMGGLVANLFNYGTYVQLVGLTIGRVLSVAGLLLLGAGVLQGARQGTRGLFNLW
                     LAAGLLHALLDAGRLSRHEDVLLPLILPACALVGIGASWAGSLPARVWRAINEQQREP
                     ASEYAVSPHTAWLLDLPEVRAEAQESRPQARPALGRSVAARTQRAGERARRASLMGIG
                     HLAVLGAIGLVAVSSWQSAWAGTQPTVDGVELTAAGQEIGAITAPGSHIVIVGPHAPE
                     LFYASGRSGWALDEESFSILEVQQLHRAGASYLLSADQTWLGQHPDYVGLLAKYSVKQ
                     LARRYILFDLNTPPSANDRLYFLESGHTLGGAFRRFWELHGSVQKLGYPISEEVSEPN
                     PLDGQVRTVQYFERAVLEHHPEFAGTRDEVMLAAVGRWVTRDRYFQAVTPFNSTADHA
                     YFEQTGHSVKEAFLRYWHQQGGLAAFGYPISEELPEISPADGKVYTVQYFERARFEWH
                     PADAGTANEVQLGLIGKQALEMRK"
BASE COUNT         1048 a         1958 c         1762 g         1011 t
ORIGIN      
        1 ctctatcaat caattctatg agaggtacgc acagggagaa cgaggctggc gcatggaggt
       61 tgcgctggga gcacctaact tgaagtcatc tcaaacacga tgctgtcgga ggccagcaat
      121 gtagaatata agcaggaaga ccccgacctt gtcattcgaa cgtagtgaga aatggcaaca
      181 cgagtcccgg tcatcccgat ccccacttcg aacacggaac gttgtcttcc ctgaaggcgc
      241 tgcccgggct atggctgtcg tcccagatac gattatgaac gtgtccggct gggcaaaagc
      301 aaaggccaca cggtactaaa cagtgtggcc tttagcgtgc atccgcccga accgcgattc
      361 aggcgcagta gtccgactca agctgcttga tcgccatgcg caggaaacgc tcctggggta
      421 acaccaggat accgtgggtg agggtacggt gggcatcctc cagttgtcct gccgccacct
      481 gcgccctcga cagcgcgagg acggcatccg ggccaagctg gtgcaccgcc actatctcag
      541 ccacgtccag gctgccgctc attcgcagcg ccagcaggaa ctcggccatg ctcgacccct
      601 gccgcccctc ccaggccagg tccaccgccc gcgccaggtg ctgccggtaa agctgttccg
      661 ggctgacgag caagtttggc tcccggcggc gcaagtttgg ctcggtgcct agcgtacggt
      721 gcgcgggggt cgggcggccc ggcgtcccgg cttgctccac ctgtcgcctc tgtggcaccc
      781 gtataggggt cggtgggggt gcataggtca cctgttcccg ttgcggcagg tgggcggcta
      841 cggaggcgat taagcgccgc ggccggaacg ccgccagtcg cgtcgccacc gcgtagaaga
      901 tcaccacacc caccagccct gagagcagga gcgcccacgg cacattgttg ctgcccgtcg
      961 tgggagaact cgcacctgcg attgactgca agggggcctc ctgctcctgc acgggggccg
     1021 gggccgcgat cactgcctct gattgctccg cctgttccgg ttgtgcgacc actccccctc
     1081 cgcccgccat ctgtggaggc agcatcgaag gcgtggaacc ctcccccagt atccagtccg
     1141 cgaccccccg cctggtgacc tcaagcactt ccgctcccgc atcggtgggc acctgcgcca
     1201 gcttggcgaa gccgaaatcg agcaacgccc cgctatcggc gtaccaccgt ggagagttca
     1261 acacgaccga gatcagccgg cgctcgcccc gcgttgccga ggttaccagg caaagcccgc
     1321 ccgcgtccgt ccagcccgtt ttgatgccgt cggccccctc gtagcgggtg agcatctcgt
     1381 tcgtgttgag gagcgggtgt ccgggcgcgt cgtagctgat ttgcttcacc acttcgttga
     1441 aggtagggaa gtgcatggcg taccaggaca ggctggcaag gtcgtacgcg cttgagtaat
     1501 gcccttccat gtccaggccg tgggggttcg caaagtggct gttccgcagg ccaagttgct
     1561 cgacccgctt gttcatcatc gcggagaagc gcgctatagg gtcttccgaa ctcgtctcgg
     1621 ggggcgcggc cttctccgcc aaccctcgcg ctacaaccat cgaggcgtcg ttgccggacg
     1681 gcagcatcat cccgtacaga aggtctaaca gcgtctgccg ctctccactg gcaaggccca
     1741 tgctcgactc gcccacgagg tcttcgtcgg tagtcgtgaa cacttcgtcc agtttgccgt
     1801 attcgagcgc cattatagcg gtgagtatct tggtggtgct ggccggggcc aactggtcgt
     1861 gcatggcgcg cgagtagaga acgcgccccg aggggtactc cactacgata gccgcgcggg
     1921 cgctgatttc cggttccgcg agcgcctcct gggtggcggg ggcggcggcg gaaattccgc
     1981 tccacgacaa ggtgaggacg aggaggagta gggaaaggag ggcggtgcgt ttggtgcggt
     2041 gcttccggga gattgcagcc atgccaggac ctcggaccaa agtaaagact ccgcttgtta
     2101 gctgtcacag ctaagcgtgt cgggagtata gcagttcaac ctatggggaa ggcacttttc
     2161 cacattgtgg aaaactcagg caaaatgtgg aaaactttga ccgttattcc gcgagccgct
     2221 ccaaaataga ttcgtacagc ggctgcacca cctgcacgaa ggccgcggtt ctttccgctg
     2281 tttcttccac gcgagccgga tccagcggcc agagcggctg cgtctcgtac agccggaccc
     2341 agcccttgtc ccacggctca agccagacgc tcccgccaag ctggaactgc acgtccagaa
     2401 ggcacctgtc tagctccctg tagacagccg cgttgtgggc ctcgtccgcc tccagatgga
     2461 agccgatttc aagcctcgcc tcacgctcgt ggacccacac ttcgtagtgc accgaggcat
     2521 tgtccccgct ccagaattgc accaacctgc tacgcgcccg cacgtgaaac tccagcgccg
     2581 aagccccaag cctctccatc acctcgctac ctacctgcga aatgaactgg gacggccgca
     2641 aacgagcgcc cggcaggagg ttcgtgcctg ttgcggtgtt cgccggcagc aagtcctctt
     2701 ccatttcgtc ttgctccccg tgcaggtaaa tcatgcgtca ggctccgttg ttgtacgaat
     2761 ttgcgcctct tgcagcgcgt ctgccacgtc gtgcagcccc agctccgcca gtcgggacat
     2821 gaaacgagtc atgacggccg cccggtgcag ccccgtgtgc cccttgcact ggtccggcgt
     2881 gagcactact tccgcgtccc gcccaatctg ggaacggagc tttccgcgca gcctctccaa
     2941 ctcccaacgc tcccagcggt cgaaggcacg accacgcaac gccctttcca ccgggccggg
     3001 gcgcggagca cgtcggtcgt ccatctccgg caggctgctc atgcccgcaa gcgcggggag
     3061 gtaagtactg gcccactcgt tcgcccgcag catccgctcg taaacgcgcg ccccgtagac
     3121 cggcaccatc tgcgccagct cgtgggcggt gaagaagtcg cgctgcgaga gttcaaggct
     3181 gccttccgag aggatgtagt tcgggcacag gtcgtctccc agcaagcgtc ccaccctcac
     3241 ccagccgatg aggccgcgcc tgcacagcca gacacgtccg gacctcgaaa tcaccaacaa
     3301 gtctatatcg tagcgcccgt tcacgttgtt gaccgccagc gcgccggtga ccgctaccat
     3361 gcgcacgaac ggcaaccgcc tcagccaccc tgcataccgc cgcgctcgct cccacacctt
     3421 ccgagaggcc tctgcccgtt cggcccgcac cgagaacaac ccctcgcgcc ccttgagaca
     3481 gtagtacgtg ccgtccgtcg ccacacgggc aagcaggtcc ggctccctgc tcaacgcctg
     3541 cgtgatttcc gccctggaat aagacgttgt gatctggtac ttgtgaatct cctcagcaga
     3601 tagcggcgcg tcgaacaggt cggcgtaggc aagagcgccg agcaccgacg agaccagggg
     3661 cagcctgtcc ccgtcggtgc ggtcgggact tggcgccgcc agtttgtttt ctagtgtggt
     3721 ccccaataag aagcctcgga tgcgacagat atgtaacccg acagtagaat gtaccggacc
     3781 gggttacatg gctcgcccgt gcccgcccgc tcggacggac gcggcatgcc ctattttcgc
     3841 atttccaggg cttgcttgcc aataagtcca agctgcacct cgttggcggt gcctgcatcg
     3901 gccgggtgcc actcgaagcg cgcccgctcg aagtactgca cggtgtagac cttcccgtcc
     3961 gcggggctaa tctcaggaag ctcttccgag atgggatagc cgaaggcggc gagtccgcct
     4021 tgctggtgcc agtaacgcaa gaaagcctct ttgacgctgt gccccgtctg ctcgaagtaa
     4081 gcatggtccg ccgtcgaatt gaagggcgtg accgcctgga agtagcggtc cctggtcacc
     4141 cagcgtccca cggcggccag catgacctcg tcccgcgttc cggcaaactc cgggtgatgc
     4201 tcgagcacgg cgcgctcgaa gtactgcacc gtgcgcacct gcccgtcgag ggggttcggt
     4261 tcgctgacct cctcggagat cggatagcca agcttctgca ccgagccgtg cagctcccag
     4321 aagcggcgga acgcaccgcc cagggtgtgc ccgctttcga ggaagtagag gcggtcgttc
     4381 gcgctgggcg gcgtgttcag gtcgaacaag atgtaccgcc gggccagttg cttgacgctg
     4441 tacttggcaa gcaggcccac gtaatcgggg tgctgcccca accacgtctg gtcggcgctc
     4501 agcagatatg aagccccggc gcggtgcagt tgctgcacct caagtatgct aaagctctcc
     4561 tcgtccagcg cccagccact acgcccgctg gcataaaata gctccggcgc atgcggcccc
     4621 acaataacta tatggctgcc gggggccgtg atagcgccta tttcctgtcc cgctgccgtc
     4681 agttccacgc catcaacagt aggctgcgtg cccgcccacg ctgattgcca gctcgacacg
     4741 gccaccaacc caatcgcccc cagcacggcc aggtggccta tacccatgag cgacgcccgc
     4801 cgcgcccgct ctccggcgcg ttgcgtcctg gcggcgacgc tcctacccag cgctggacgt
     4861 gcctgcggac ggctctcctg cgcctcggcg cgcacttcgg ggaggtccag cagccaggca
     4921 gtgtggggcg aaacggcgta ctccgacgca ggttcgcgct gctgctcgtt gatggcgcgc
     4981 cacacgcggg cgggtagcga accggcccac gaggccccga taccgacaag ggcgcacgcg
     5041 ggcaaaatca gcggcaggag cacgtcttcg tggcgcgaga gcctgccggc gtcgagcagc
     5101 gcgtgcaaga ggccggccgc gagccaaagg ttgaacaggc cacgagtgcc ctgcctcgcc
     5161 ccctgtagca ctcccgcccc caggaggagc agccccgcca ccgacagcac ccgccctatc
     5221 gtcaggccca ccagctgaac gtaggtgcca tagttgaaca ggttggcgac cagccccccc
     5281 atgcttccgg ccccatcgcc gctgcccagc acaagagtat cgaccttgga cgagtagagc
     5341 caccagccgc ctgcagcaat aaccagcatc gcagagtaga ccaggacttt gccccggtgc
     5401 gccgacttgt gccaggcatc gctccacgtg tccgcagtgg cgcgcggcgt gcgccttgtt
     5461 atgcggcctt gcaacctttc cccgtccacc gacagcacga gataggcagc gggcaccgcc
     5521 aggaagatgc tgccagggtc gagaagcgcc gctatggctc ctactgtgat ggcgacgagg
     5581 aagcggcctg ttgaaccctg gacgcgggcg tggtttacag tgtcgcgcca gctaaggaga
     5641 gccagcacag ccagcgcctg catcgcaagt atcagggcgg cgggcgagaa ttgctgtccc
     5701 agtgccgcgc tcagggggga gaccgcgaag aatagcgtgg cgtacaccgc cgctaacgaa
     5761 cctgccgtgc ggcgcaccg
//
LOCUS       DASRWZ010000227         6644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_634904, whole genome shotgun sequence.
ACCESSION   DASRWZ010000227 DASRWZ010000000
VERSION     DASRWZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634904"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..362
                     /locus_tag="VFH60_03550"
     CDS             111..362
                     /locus_tag="VFH60_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312887.1"
                     /translation="MFLGWFDDTPKKAAAEKLEEAVERYVAKFGEAPDLCLVNQKDAT
                     THAGVEVKVVEYVRPNHFWVGKASALPGGTGVGSIVQAA"
     gene            complement(648..1415)
                     /gene="minC"
                     /locus_tag="VFH60_03555"
     CDS             complement(648..1415)
                     /gene="minC"
                     /locus_tag="VFH60_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HET6312888.1"
                     /translation="MQTTENVIIKGVREGLLFILDDDVPFAHILQELVQRINGQPNFF
                     KGAGVILNAGRRVLETGDFDRLYKMLSRNDMKVLSFVGASAQSRMVAESFGVTSRPPS
                     FAAGDSGVSLGLPNRGTRPGLGGMLAQAAADGNVADADTGLFLRATLRHGQTVRYPGD
                     VCILGDVEAGAEVIADGDVVVWGTLRGLVHAGANGDDEAVVCALQMSPVQLSIAGVVS
                     RFPSHTSRYPDAPQPPELARLEGGRIVIEGWQGSVVE"
     gene            complement(1572..3848)
                     /locus_tag="VFH60_03560"
     CDS             complement(1572..3848)
                     /locus_tag="VFH60_03560"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET6312889.1"
                     /translation="MLNETVKRKYTPMYVVMGLMFALLAGQLYKLQIVEGGRYVALAE
                     NNRLRTIPVKANRGVIYDRNAQRLVLNNPSYNVLVTAADLADVNCAVQNIEGSKVIQD
                     LAVILAPRVDGVAKVPYVIALKPAELAVANEKAVEATGVNRQGEVANLLSNILQLNAD
                     NLRAPINDTMAHYATSGSIFLIRKDVTAEQATAIQAEADSLPGISVYTELEYNFIKGF
                     EDCLKPVVVQQVSYDAMQSVVVQITKLRGVSVSPEPMRHYVDGSLYSHLLGYVGQISE
                     EQYLAAQEKYGEEANPYEKGDKVGKAGLEATLDEQLRGKKGVQSMIVNSHEQVVSEVA
                     YQAPITGNNVTLTLDSSLQKAATQAMHKGIDSAKVKAGVAIVMRVDDGQVLAMVSLPS
                     YDNNLFTGGIKQTEFDALMQDPTLPLFNRAISGAYPPGSTFKMITAAAALQEGVVNVD
                     TKFVCPGLIQVPLSTNENVRTPFRDWRPQGHGTINVVEALTVSSDVFFYIVTGPRQED
                     NVVKKSDGTRDITWLRYYVPGARQPIEFNGLGIERLHNYATAFGLGSKTGIDLPGETD
                     GLAPNREWKTNRDPVNGWSLGDTLFTAIGQGDNLVTPLQLVNVTAAVANGGTLYKPQL
                     VQKITGPNGEVVQDYQPQVLGKVPVSPENLAIVREGMRQVVVNQAKGTAATHISLKSF
                     EWAGKTGTAEFGEPIRIDKGKEVRRAHAWFTAFAPYDKPEIAVVVLLEGGEESLEGST
                     FAVPVTDEILKAYFKVDH"
     gene            complement(3909..4415)
                     /gene="mreD"
                     /locus_tag="VFH60_03565"
     CDS             complement(3909..4415)
                     /gene="mreD"
                     /locus_tag="VFH60_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET6312890.1"
                     /translation="MVYVGAIILFLAALVQSVVLPQAVPISARPHFVVLLVVAVCIAE
                     SLYEAVIWGFIGGLMLDLLSGPAFPIGTNALILVLLALLASMGQADPFHNLLLVPLVT
                     VFTATVFYHVLMMGVSLALGHSVALLDTMLRVALPGAVLNAILMPVAYSAMLWLSERV
                     GRRVRVEW"
     gene            complement(4487..5326)
                     /gene="mreC"
                     /locus_tag="VFH60_03570"
     CDS             complement(4487..5326)
                     /gene="mreC"
                     /locus_tag="VFH60_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET6312891.1"
                     /translation="MKQKERSLSWTRTLYLLVALVLVSLALILLSQGKQLGPVEGAVN
                     AVVTPVQQFTRDVTTGIGSWIDALSRAHELEDENRRLRGLVEALTTENASKEAAIREN
                     EQLRQALKFQTDRPDITGVLANNIGGDPSSQREILTIDRGSAYGIKQGMAVLSPGGIM
                     VGTIDEVKQDRATVLLITDQESRVPVTTQRTQTPGILEGMWQHGSALRVAHIPRDADV
                     AKDDLLLTSGLGGTFPRGIIVAQVDVVHQDDVQTEKSAEALPLVETYSLESVLVVTNE
                     GTP"
     gene            complement(5412..6482)
                     /locus_tag="VFH60_03575"
     CDS             complement(5412..6482)
                     /locus_tag="VFH60_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET6312892.1"
                     /translation="MIGPLNALFGLFSRDLGVDLGTANTLVHVRGKGIVVSEPSVVAI
                     DKRTRKPYAVGAEAKAMVGKTPGYIVAIRPLKDGVIADFDIVEMMLKHFIRKVHSQGL
                     MTPKPRVVIGIPSGVTEVERRAAHDAAINAGAREAYPIEEPMAAAIGAGLPVQEPVGS
                     MIVDIGGGTTEVAVISLGGIVINHSIRTAGDEIDESIIQYARREHNLHIGERMAEAAK
                     IAAGSAYPLEEELAVTLRGRDVITGLPKAIDISSVELRDAISQPVSEIVEVVRLALEE
                     TPPELVADIMQFGITLAGGGALLKGLDRRLMLETKMPVRVAEDPLTCVVRGAGRIVES
                     LEKYDRVLKSSAQNRRAVVVQN"
BASE COUNT         1215 a         2002 c         2062 g         1365 t
ORIGIN      
        1 tgaagtgtgc atttcgtgta ttgacaggca tggggaggtg tgctacaatt agaacagaag
       61 ttctaagttt gtgggagaat aaatgcagtt tgagtttgtg gaggtgtagc atgtttcttg
      121 gttggttcga tgacactccg aagaaggccg ccgcggaaaa gctggaagag gcggtcgagc
      181 ggtacgtggc taagttcggt gaggcccccg acctgtgcct ggtgaaccag aaggacgcca
      241 ccacccacgc cggggtggaa gtgaaggtgg tggagtacgt caggccgaac cacttttggg
      301 tcggcaaggc ctcggcgctg cccggcggca cgggcgtggg cagtatcgta caggcggcgt
      361 aggtagaggg acggaagtga gaagagaagg ggtaggcgta ggtacctgcc ccttctttgt
      421 ttgtaggaaa tcggctgctg gggtgtctgg ggtcgccgtg ggatagccct gtgggggagg
      481 tcctgtggag gtcgccctgt ggctaaagcc acgggctggg aagtagcgaa gtctccttcg
      541 gagactaccg gaggcgagag tagtcccgta gggactttgc ctcttctcag cccgtggctt
      601 tagccacagg gcatagtggc agggcgtccc gatacacctg gctgagacta ttccaccacg
      661 cttccctgcc agccctcgat cacaattcgg ccgccctcca gcctggcaag ctccggcggt
      721 tggggcgcat cggggtagcg gctggtgtgg ctggggaagc ggctgactac gcccgcgatg
      781 ctgagttgca cgggcgacat ttgcagagcg cataccaccg cctcgtcgtc gccattcgcc
      841 ccggcgtgca ccaggccgcg cagggtgccc cagaccacca cgtccccatc cgcgattacc
      901 tcggcccctg cctccacatc acccaggatg cacacgtcgc ccggatagcg caccgtctgc
      961 ccgtggcgca gggtcgcacg aaggaacagg ccggtgtcgg cgtccgcgac gttgccgtcc
     1021 gcggcggctt gggccagcat gccgcccagt ccgggccggg tgccgcgatt aggcaggccg
     1081 aggctgacgc ccgagtcgcc cgccgcgaac gaaggggggc ggctggtcac gccgaagctc
     1141 tcggccacca tgcggctctg cgcggacgcg ccgacgaacg agagcacctt catatcgttg
     1201 cgggagagca tcttgtacag gcggtcgaag tcgcccgttt cgagcaccct gcgccccgca
     1261 ttgagtatga ccccggcccc cttgaaaaag ttgggttggc cgttgatgcg ttgcaccagc
     1321 tcttgcaaaa tatgcgcgaa aggcacatcg tcgtccagga tgaacaacag cccttcgcgt
     1381 acccccttga tgatgacgtt ctctgtcgtc tgcaaacctg gcctccgaga gcaagtaatg
     1441 agtgatgagg actgaggact gagtcgtcct tgcccggcga tctatgtgca gtttaccagt
     1501 ctctgcacca acgcactacg ttttgcgcaa cgcgagctac tcagtcctca gtcctcatca
     1561 ctcattactt cctaatggtc caccttgaag taagccttga ggatttcgtc ggtgacgggc
     1621 acggcgaagg tggagccttc gaggctttcc tcgccgcctt ccagaaggac cacgacggcg
     1681 atttcgggct tgtcgtaggg agcgaaggcg gtgaaccagg cgtgggcgcg gcggacttcc
     1741 ttgcccttgt caatgcggat tggctctccg aactcggctg tgccggtctt gcccgcccac
     1801 tcgaagctct tcaggctgat gtgagtggca gcggtgccct tggcctggtt taccaccacc
     1861 tgccgcattc cctcgcgcac tattgccagg ttctcaggcg acacgggcac cttgccgagc
     1921 acctggggct ggtagtcctg caccacttcg ccgttgggcc cggtgatctt ctgcaccagt
     1981 tgcggcttgt agagcgtgcc gccattggcg acggccgccg tcacgttgac gagttgcaga
     2041 ggcgtcacca ggttgtcgcc ctggccgatg gctgtaaaga gggtatcgcc cagagaccag
     2101 ccgttcacag ggtcgcggtt cgttttccac tcccggttgg gagcgaggcc gtcggtttca
     2161 cccggcaggt cgatgcctgt cttgcttccc agaccgaacg cggtggcgta gttgtgcagc
     2221 cgctcgatgc ccagtccgtt gaactcgatg ggctgacgcg cgccgggaac atagtaccgc
     2281 aaccatgtaa tgtcacgtgt gccgtcgctc ttcttcacga cgttatcttc ctgccgcggc
     2341 ccggtaacga tatagaagaa cacgtcgctg gaaacggtaa gggcctctac cacattgata
     2401 gtcccatgac cctggggccg ccaatcgcgg aatggagtgc gcacgttctc gttagtcgag
     2461 agtggcacct gtatgaggcc ggggcaaacg aacttggtgt ccacgttgac gacgccttcc
     2521 tgtagcgccg ccgccgccgt gatcatcttg aaggtcgagc cgggggggta ggccccactt
     2581 atggcgcgat tgaagagggg gagggtcggg tcctgcatca gcgcgtcgaa ctcggtctgc
     2641 ttgatgcccc cggtgaacag gttgttgtcg taggagggca gcgacaccat cgcgagcacc
     2701 tgcccgtcgt ccacccgcat cacaatcgcc acgcccgcct tcactttggc gctgtcgatg
     2761 cccttgtgca tcgcctgggt ggctgccttt tgcaggctcg agtctagggt aagggtaacg
     2821 ttgttgcccg tgataggggc ctggtacgcc acttcggaga ccacttgctc gtgcgagttg
     2881 acgatcatgc tctgcacgcc cttcttgccg cgcaactgct cgtccaacgt cgcttccagc
     2941 ccggccttgc ccaccttgtc gcctttttcg taagggttgg cttcctcgcc atatttctcc
     3001 tgcgcggcga ggtactgctc ctcggaaatc tggcccacgt agcccagcag gtgcgagtag
     3061 agcgacccgt caacgtagtg tcgcatcggc tccggggaga cgctgacgcc gcgcagcttg
     3121 gtaatctgca ccaccacgct ctgcatggca tcgtagctca cctgctgcac cacgaccggc
     3181 ttgagacaat cctcaaaccc tttgatgaag ttgtactcca gctcggtgta gacagatatg
     3241 ccgggcaggc tgtccgcctc ggcttgtatc gccgtggcct gctcggcggt cacgtccttc
     3301 ctgatgagga agatgctccc gctggtggca taatgcgcca tcgtgtcgtt gatgggcgcg
     3361 cgcaggttgt cggcgtttaa ctgcaagatg ttggacagca ggttagccac ctcgccctgc
     3421 ctgttcaccc cggtagcctc taccgctttt tcgttcgcga cggccaactc agcgggcttg
     3481 agcgcgatga cgtagggcac cttcgcgacc ccgtccaccc tgggggccag gataaccgcc
     3541 aggtcttgta tcaccttgga gccttcgatg ttctgcacgg cgcagttcac gtcggccagg
     3601 tcggcggccg tgacgaggac gttgtacgag gggttgttaa gcaccagccg ttgggcgttg
     3661 cggtcgtaaa tgaccccgcg gttggccttg accggtatgg tacgcaggcg gttgttctcg
     3721 gccaacgcaa cgtacctgcc accctctacg atttgcagct tataaagctg tccggcgagc
     3781 aaggcaaaca tgaggcccat gaccacgtac atcggggtgt atttgcgttt tacagtctcg
     3841 tttagcatct tttggatttt agattttgga ttttggattg ttgagcctga tagcgaacaa
     3901 gtacgcccct accattctac gcgtacccgg cggcctaccc gctcgctgag ccagagcatg
     3961 gcgctgtagg ctaccggcat cagtatggcg ttgagcacgg ccccgggaag ggcgactctc
     4021 aacatggtgt ccaacagagc cacgctatgc cccagcgcca gcgacacgcc catcatcaat
     4081 acgtggtaga agaccgtggc cgtgaaaacc gttaccaggg gcaccagcaa caggttatga
     4141 aacgggtcgg cctggcccat gctggctaga agggccagca gcaccagtat aagggcgttg
     4201 gtgccaattg ggaaggccgg gccgctcagc aagtcgagca tcagtccccc tatgaaaccc
     4261 catattaccg cctcgtacag gctctcggca atgcataccg ccaccaccag cagcacgacg
     4321 aagtgcggcc gtgccgatat aggcaccgcc tgcggaagca ccaccgactg cacgagggcg
     4381 gcaagaaaaa ggattatcgc gccaacatat accatgcttc aagagtaaca cgtaacgcgt
     4441 aacacgtaag gtccacctat agaggttacg cgttacgtgt tacctcctaa ggtgttcctt
     4501 cgttagtgac taccagcacg ctctccaggg agtacgtttc gaccaggggt agggcctcgg
     4561 ccgatttctc ggtctgtacg tcgtcctggt gcactacgtc tacctgggcg acaataatgc
     4621 ctcgcgggaa ggtgcctccc aggccggagg tgagcagcag gtcgtcttta gcaacgtcag
     4681 cgtcgcgggg gatatgggcc acgcgcaacg cgctaccatg ctgccacatg ccttcgagta
     4741 tgccgggcgt ttgcgtgcgc tgggtggtca cgggcacccg cgactcctgg tcggtgatga
     4801 gcagcacggt agcccggtcc tgcttcacct cgtcaatagt gccgaccatg atgccgccgg
     4861 ggctgagcac ggccataccc tgcttgatac cgtaggcgct gccgcggtct atcgtcagta
     4921 tctcacgctg gctgctgggg tcgccgccga tgttgttggc gagcactccg gtaatatcag
     4981 ggcggtcggt ctggaacttg agggcctggc gtagctgctc gttctcgcgt atcgccgctt
     5041 ctttgctggc gttctcggtc gtcagcgcct cgaccaggcc tcgcagcctg cggttctcgt
     5101 cctctagctc gtgggcgcga gacagcgcat cgatccagct tcctatacct gtagtaacgt
     5161 cacgcgtgaa ctgctgcacc ggagtgacca cagcgttgac cgcgccctcg accggcccaa
     5221 gctgcttgcc ctggctcaac aaaattagcg ccaacgaaac aagcaccagc gccacaagca
     5281 aatagagggt gcgcgtccag gagagtgagc gttctttctg cttcatgaga ctttcgattt
     5341 tggattttag attttagatt ttggattagc tgctacaatc caaaatctaa aatctaaaat
     5401 ccagaatccg attagttctg tactacgacg gcccggcggt tctgcgcgct ggactttagc
     5461 actcggtcgt acttttcgag cgactcgact atgcggcccg cgccacgcac cacgcaggtg
     5521 agggggtctt cggccacgcg cacgggcatc ttggtctcga gcatgaggcg gcggtcgaga
     5581 cccttgagga gcgcgccgcc acctgcgagg gtgatgccga actgcatgat gtcggccaca
     5641 agttccggcg gggtctcttc gagggccagg cgcaccacct ccacgatttc ggagacgggc
     5701 tggctgatgg catcccgcag ctccacgctg ctgatgtcaa tcgccttggg gaggccggta
     5761 ataacgtcgc gcccgcgcag ggtgacggca agctcttctt cgagggggta ggcagaaccg
     5821 gcggctatct tggcggcctc ggccatgcgc tcgcctatat gcaggttgtg ctcgcggcga
     5881 gcgtactgga tgatcgactc gtcaatctcg tcaccggcgg tgcggatcga gtggttgatg
     5941 acgatgccgc cgagggagat gacggccacc tcggtggtgc cgcccccgat gtccactatc
     6001 atcgagccaa ccggctcctg gacgggcagc ccggccccga tagcggcggc cataggctcc
     6061 tcaatggggt atgcctctcg cgccccggcg tttatggccg catcgtgggc ggcccggcgt
     6121 tccacctcgg tcacgccgga ggggatgcca atcaccacgc ggggcttagg cgtcatcagc
     6181 ccctgcgagt gcactttgcg gatgaagtgc ttgagcatca tctccacaat gtcgaagtct
     6241 gcgatcacac catccttgag gggacgtata gcgacgatgt aaccgggggt cttgcctacc
     6301 atcgccttgg cctctgcacc cacggcgtag ggcttgcggg tgcgtttatc gatggctacg
     6361 acggaaggct cggacaccac aatgcccttg cctctaacat gtacgagagt gtttgccgta
     6421 cccaggtcca cacccaggtc gcgactgaac aggccgaaca gagcgttcaa agggccgatc
     6481 ataagcgtag tgctcctatc ggcgggcaaa ccacaagcag aagaagtgtg ccaaccgcaa
     6541 agttgaaggt cggtggccgg tcgcgcgtga aacttcagta gtatacacgc agcgggccat
     6601 gagcagcgca tctccgcgcc ggagttaaga ctgtcgtgag gcag
//
LOCUS       DASRWZ010000228         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_429282, whole genome shotgun sequence.
ACCESSION   DASRWZ010000228 DASRWZ010000000
VERSION     DASRWZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429282"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1649
                     /locus_tag="VFH60_03580"
     CDS             237..1649
                     /locus_tag="VFH60_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6312893.1"
                     /translation="MATITANKAAPVTDPATLKTPAGPKGAPVLGSIRAMRKDPLRFL
                     LSVSRRYGDVTQYHFLRLTQYMVNHPAGVKRVLQENNRNYTKSVFDYQILKSALGQGL
                     LTNDDDSWLSQRRLMQPAFHRQRLAAFGTLMTEATTRMLDERWGRFAASGRELDVSAE
                     MHELALRIVGNALFGLDLTTEADSVGRALTIAQRRLIDQFFMPYIPMWVPTPKHLEFR
                     RAIRDIDEVVFKIIEERRRNDEDRGDLLSMLMLARDADTGEGMDDQQLRDEVITLLLA
                     GHETTANALTWAWYLLSQNPLAARRMREELREVLGGRVPTMSDLPNLTYTRMVLDETM
                     RLYPPAWSISRKATEEDELAGYRIPAGAFITVSPYAMHRHPRYWENPEGFDPERFTPE
                     RSAQRPPYAYFPFGGGPRLCIGNNFALMEGQLILATMAQRYRLDLVPGHPVVVEPLIT
                     LRARYGMRMQLHPLGDGKVA"
     gene            1653..2249
                     /locus_tag="VFH60_03585"
     CDS             1653..2249
                     /locus_tag="VFH60_03585"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6312894.1"
                     /translation="MATRTALPAERKRQILDAAASVFSRAGYYEARMDDIADEAGLSK
                     GALYLYFTGKEALSIALVEGHLDHSLRFMEGLVSAEGTVRDRLMSYIRHESEELESMR
                     AILPVCYEFYAAALRQEEAAAFFKSYFRRYLDLLAEMVQQGTDRGELRQADPRQTAIL
                     LAGSHEGLTLLWLMDPTAFDWTAQMESGLQLVLDALVV"
BASE COUNT          433 a          807 c          701 g          382 t
ORIGIN      
        1 gatgttcctt agtcccctac ggggactttg ccccttgtca gccctgtggc taaagcagga
       61 cgaacataga cggactaacc accccgttcc atcgacaagc atgtcaagca tgtcaccctg
      121 gcaggcactt cattttacct attgacaggt acctgggatg gtgctactat cataatgact
      181 gaccagtcag tcatcactcc gaacggcaag tagcacgcat aaagtggagc accccaatgg
      241 caactatcac cgcgaataaa gcggcccctg taacagaccc cgccacactg aaaacccctg
      301 ccggaccaaa gggggcgccg gtcctcggca gcatccgcgc catgcgcaag gacccgttgc
      361 gcttcctgct cagcgtctcc cgccgctatg gcgacgtgac ccagtaccac ttcctgcgcc
      421 tcacacagta catggtgaac cacccggcgg gcgtgaagcg cgtcttgcag gagaacaacc
      481 gcaactacac caagagcgtc ttcgactacc agatcctcaa gtcggcgttg gggcaaggtt
      541 tgctcaccaa cgacgacgac tcgtggctga gccagcgccg cctcatgcag cccgccttcc
      601 accgccagcg cctggccgct ttcggcacgc tgatgaccga ggctaccacc cgcatgctgg
      661 acgagcgttg gggccggttc gccgcaagcg gccgcgagct ggacgtgtcc gccgagatgc
      721 acgagcttgc gctgcgcatc gtgggtaacg ccctgttcgg cctcgacctg accacggagg
      781 ccgattctgt gggtcgcgcc ctcaccatcg cgcagcgccg cctgatagac cagttcttca
      841 tgccgtacat accgatgtgg gtgcccaccc ccaagcacct cgagttccgc agggcaatcc
      901 gcgacattga cgaagtggtc ttcaagatca tcgaggagag gcggcgcaat gacgaggacc
      961 ggggcgacct cctctcgatg ctcatgttgg ctcgcgacgc ggatacgggc gagggtatgg
     1021 acgaccaaca actgcgcgac gaggtgatta ccctgctcct cgcggggcac gagaccaccg
     1081 ccaacgccct cacctgggcc tggtacctgc tctcccagaa ccccctcgca gccaggcgca
     1141 tgcgggagga gctacgggaa gtgctcggcg ggcgcgtgcc caccatgtcc gacctgccca
     1201 acctgaccta cacaaggatg gtgctggacg agacgatgcg cctctacccg cccgcgtggt
     1261 ccatcagccg caaggccacc gaagaggacg agcttgcggg ctaccgcatc ccggcgggtg
     1321 ctttcatcac ggtcagcccc tatgcgatgc accggcaccc ccgctactgg gagaacccgg
     1381 aaggcttcga ccccgaacgc ttcacgcctg agcggtcggc gcagcgaccc ccttatgctt
     1441 atttcccctt cggaggcggg ccgcgcctgt gcatcggcaa caacttcgcg ctcatggagg
     1501 gccagttgat cctggcgacg atggcgcagc gctaccgcct cgacctcgtg ccgggtcacc
     1561 cggtagtggt cgagccgctt atcaccctcc gggcgcggta cggcatgcgc atgcaactcc
     1621 accccctcgg agacgggaag gtggcgtaac agatggcgac ccgcacagcc ctccccgcgg
     1681 agcgcaagcg ccagatactc gacgccgccg cctcagtctt ctcccgcgcg ggctactacg
     1741 aggcccgcat ggacgacatc gcggacgaag cgggcctcag caagggcgca ctctacctct
     1801 acttcacagg caaggaggcc ctcagcatcg cgctggtgga ggggcacctg gaccactcgc
     1861 tgcgcttcat ggagggcctc gtcagcgccg agggcaccgt gcgcgaccgg ctgatgtcgt
     1921 acatcaggca cgagtcggag gaactggaga gcatgcgggc catcctgccg gtgtgctacg
     1981 agttctacgc ggcggcgttg cggcaggagg aggcggccgc cttcttcaag agctacttcc
     2041 ggcgctacct cgacctgctg gccgagatgg tgcaacaggg caccgaccgg ggcgagctac
     2101 ggcaggcaga cccccgccag actgccatcc tcctcgcggg cagccacgag ggcctcaccc
     2161 tgctctggct gatggaccct accgccttcg actggacggc ccagatggaa agcggcctgc
     2221 aacttgtgct cgatgccctg gtggtgtagc gggggtgcag gggtactccc ctgacggggg
     2281 gctgggggtg tcccccaact ctctcccctc gtcgtaactt cct
//
LOCUS       DASRWZ010000229         6955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1320342, whole genome shotgun sequence.
ACCESSION   DASRWZ010000229 DASRWZ010000000
VERSION     DASRWZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320342"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1346
                     /locus_tag="VFH60_03590"
     CDS             81..1346
                     /locus_tag="VFH60_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6312895.1"
                     /translation="MSLHTDPLQPANVRVPRDRLSRLYGIIRSLNSIIQLDKLLNQIM
                     ASAAELIAAEGGSLLLVSPDGGSLTFRVACGPAAGQLVGLTLPVNDRSVVGLVALLGT
                     PIIENDTATSPHFSGQVDKKTGYTTRKLLCVPLKVQGRIIGVVELVDKVSGQDFHAED
                     VELLEVLADAAAIAIENARLYEEQLLKAEQLEQAYGELKEAHRATLQTLAGILDARDD
                     ATHGHSRRVVTFTLKLAKMLGITDRRQLKSIEQGALLHDVGKIGIADAVLRKPGPLTE
                     EEWEEMRRHPEIGYKLLKNLGFLRETLPIVRFHHEHWDGSGYPCGLKGEGIPLEARIF
                     SVVDAFDAITSDRPYATPRTYEEACEILVAESGVKFDPAIVEAFLSGPAHEWERLREE
                     ATGLLDPGLVLHLYATGPLSLYALPQRPG"
     gene            1476..3404
                     /locus_tag="VFH60_03595"
     CDS             1476..3404
                     /locus_tag="VFH60_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET6312896.1"
                     /translation="MHRENQLMPNQARSNIGSIFSSAVSFLTRRLRNQIIVPYLVLAT
                     VFAMAGTYLLLNSANESLHARFNNQLLDAARGAADGLVKSEMDQLAGLRAIVYTQGFS
                     QALAGGDATAVRGLAAPQAVNYGFDSLLVVKADSSVLLDLSQDQRAPSAGVPAGMSTL
                     VSAALSPSGALDKVSSVVATSSGPVFYTAGPVRHGDKVVGAVLVGTDLSRLMRQLARS
                     SLSDGAGFYSGGEAPRYMLTSSDTAPSIPALPGGWYEEVLGKATDQIRLRTLALPAGT
                     YVEALGVVPGNGVGGSPVGVYGVMLSTSTLDANLRETLWKLLPLFALGLLLIVFIGHS
                     IASGIHRPVAQLVNASNDVAGGDLDVQIPATRQDELGVLAARFNVMVSGLRHSLFVKD
                     LFGRFVSPEVSAKLLAGQIELGGEQREVTVLFSDLRDFTRLSEQYSAPAIVDLLNEHF
                     SNVIQAAQENGGIVNKFGGDSTLIIFGAPVDMPDHADRALATAVAMQAALGRLNERRT
                     KEGWAPLRQGIGVNTGTVVAGQIGSKARMEYTVIGDAVNLASRLQSMTKDLNGCDIVF
                     SENTLLALSDPPEWLVEDLGPIEVRGKSKQVRAYGLVGLAEGDVEAAHEPDKLAVLPE
                     RAPALENVPAVVGVGQAR"
     gene            3404..4510
                     /locus_tag="VFH60_03600"
     CDS             3404..4510
                     /locus_tag="VFH60_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312897.1"
                     /translation="MQAGPVRPRRSTAGGDMRVLLLGSGLALLALFATAWWALQRPTP
                     PWLKGEVIAYRVADYGRDETAPVYVPLSADIVQAVIKDNATFQRDGRDFAPPSQSAAQ
                     SVAWLATETPERGALPDQAVGGTTTPTRVPTNAATPMPQASTHTATTTRTVTAMSTPV
                     ATGTPAGGQGPTQEANAEVTWTPSATATRTLPGVPTATQEATPSPATKRGDVEATATP
                     KPTSEADPGKNKAKPSATATVGRTSRVPTSTSTPPPAPSDTPTPTAALPSDTPTPAPT
                     DTPQPTPSATPSLTPSATPSDTPTIAPPTEEATATPDAKPTHEPKPTDEPKPTKEPKA
                     TEEPRPTREPKPTDEPAPTKEPKPTKEPNGSALP"
     gene            complement(4614..5243)
                     /locus_tag="VFH60_03605"
     CDS             complement(4614..5243)
                     /locus_tag="VFH60_03605"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET6312898.1"
                     /translation="MEKDAEYPMVTLTPGELHPREAYRLLVSIVVPRPIAWVSTLGAD
                     GSHNLAPFSFFNVVGGTPPPVMVSVGQRTGRPKDTLRNVQETGEFVVHIANEGLAARL
                     NLTSGEWPYDADEFDLAGLTRVPSTDVRPPRVLEAPVALECRNTQIVPVADTHYTMIL
                     GQVLRYHIREGLLGANGLVQAIQLGAIARLGGDEYTAIDRTFSLTRPQV"
     gene            complement(5293..6030)
                     /locus_tag="VFH60_03610"
     CDS             complement(5293..6030)
                     /locus_tag="VFH60_03610"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET6312899.1"
                     /translation="MMIVAAARALQGARTVFVGVGLPNLACNLALRTAAPDLQLIYES
                     GVYGARPARQPLSIGDPALVSGALSVVSMPDLFGLYLQGGLIDVALLGGAQVDRFGNL
                     NTTVIGDYDGPRVRLPGSGGACEIAINARRLIYIMRLSRRSFVSALDFRTSPGHLSGG
                     EARGASGMPGEGPVTVVTDLAVFDFDNPQHEMQLSGLHSGVSLEQVQQEVGWDLRVSS
                     TLAETPPPGDTELDLLRKELDPDSHYR"
     gene            complement(6060..>6955)
                     /locus_tag="VFH60_03615"
     CDS             complement(6060..>6955)
                     /locus_tag="VFH60_03615"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET6312900.1"
                     /translation="REAISTYVRSGQTLALEGFTASIPFSAAHEIIRQGHSGLTLARM
                     TPDLVYDQLVAAGLARKLVFSYLGNPGVGPLLAIRRCIEQGKPVPLELEEYSHYGMVG
                     RYMAGAYRLPFFPLRSYTGTDLPLVNPLIRSVQSPYGDGEVAVVPPLNPDVAILQAQR
                     ADVEGNTQMWGLLGAQKEVAFAAKRVIVVVEEIVDEAVIRADPNRTVIPGLIVDAVVH
                     EPFGSHPSYVQGYYDRDNAFYLEWDEISRDEARTAEWLREWVYEVPDRATYLRKLGPE
                     RLARLRPGPAPSPPVDYGDYR"
BASE COUNT         1291 a         2262 c         2213 g         1189 t
ORIGIN      
        1 agggggataa gcaccagggt aaacagtgcc ccgcaaccga gccgtacctt gaattacgag
       61 gcaggcgttg tgccacgcaa atgagcttac atacagatcc attacagccc gctaacgtgc
      121 gcgtacctcg cgaccgcttg agcaggttat acggcatcat ccgctcgctc aactcgatca
      181 tccaactcga caagctgctg aaccagatta tggcttcggc agccgagtta atcgccgcgg
      241 agggagggtc gttgctcctg gtaagccccg acggcggcag cctcaccttt cgcgtggcct
      301 gcggccctgc ggcgggccag ctcgtcggcc tcactctgcc cgtaaacgac cgcagcgtgg
      361 tgggtctcgt ggccctgctc ggcaccccca ttatcgagaa cgacaccgcg accagtcccc
      421 atttctccgg gcaggtagac aaaaagactg gctatacgac gcgcaagctg ctctgcgtgc
      481 ccctcaaggt gcaaggcagg attattggcg tggtggaact ggtggacaag gtctccggcc
      541 aggacttcca tgccgaggac gtggagctac tcgaagtgct ggcagacgcg gcggccatcg
      601 cgattgaaaa cgcccgcctc tacgaagaac aactgctcaa ggcggaacaa ctggagcagg
      661 cgtacggcga gctaaaggaa gcccaccgcg ccacgttgca gaccctggca ggaatcctcg
      721 acgcccgcga cgacgccacg cacggccaca gcaggcgggt cgttaccttt accttgaagc
      781 tcgcgaagat gctgggcatc accgaccgcc gccagctcaa gtccatcgag cagggggctt
      841 tgctgcatga cgtaggtaag ataggcatag ccgacgccgt gctccgcaag ccggggccgc
      901 tcaccgagga agagtgggag gaaatgcggc gtcaccccga aatcggctac aagctgctga
      961 agaacctcgg tttcctccgc gagaccctgc ctatcgtgcg cttccaccac gagcactggg
     1021 acggcagcgg gtacccttgc ggcctgaagg gtgagggcat ccccctggaa gctcgcatat
     1081 tctctgtggt cgacgcgttc gatgccatca cgtcggacag accctatgcg acgccgcgca
     1141 cttacgagga ggcttgcgag atactagtcg cggaaagcgg cgtcaagttc gatcccgcga
     1201 tagtcgaggc tttcctctcg gggcccgctc acgagtggga gcggctcagg gaggaggcga
     1261 ccggcttgct cgacccaggc ctggtcttgc acctgtacgc caccggaccg ctgtcgttgt
     1321 acgcccttcc acaacggccc ggttgagcgt cgcgtgccgt gcgcctgcgc cctggaccag
     1381 gcaggcgttg ctagtacaat tcggccatgc tcatatactc ctgtgtagct atatccgcgt
     1441 gctaagcacg cgcctataat gcagggagtg ggtctatgca tagggagaac cagttaatgc
     1501 ccaaccaggc gcgttccaat atcggctcta tctttagcag cgccgtcagc ttcctgacgc
     1561 gtcggcttcg caaccagatc atcgtgccat acctggtgct cgccaccgtg ttcgccatgg
     1621 cgggcacgta cctgctgctc aactcggcca acgagtcgct gcacgcgcgt ttcaacaacc
     1681 agttattgga cgcggcgcga ggggcagccg acggcctggt gaagtctgag atggaccaac
     1741 tggcgggcct ccgggctatc gtctacacgc agggcttcag tcaggccctt gcgggcgggg
     1801 acgcaaccgc ggtgagaggt ctcgctgcgc cacaggctgt caattatggc ttcgacagtt
     1861 tgctcgtagt caaggccgat agttctgtgc tcctggacct ctcgcaggac cagcgtgcgc
     1921 cttcagcggg tgtgcctgcc ggcatgtcga cccttgtctc cgcggccctc agcccttcag
     1981 gtgcgctcga caaagtatcg tctgtggtgg cgacttcgag cggcccggtc ttctacaccg
     2041 ccggacccgt acgccacggc gataaagtcg tgggggccgt gctggtgggc accgacctct
     2101 cccgtctgat gcgccagctt gcgagaagca gcctgagcga cggcgcgggc ttctacagcg
     2161 gcggagaagc tccccgctac atgcttacct cgtccgacac cgcgccctca attccggcgc
     2221 tacccggtgg ctggtacgag gaagtgctgg gtaaggccac cgaccagatc cggctgcgca
     2281 cgctggccct gcccgccggc acctacgtgg aggccctggg cgtggtgccc ggcaatggcg
     2341 tgggcggctc tccggtgggc gtgtacggtg tgatgctgag cacgagcacc ctggacgcca
     2401 acctgcgcga gaccctgtgg aagttgctcc ccctgttcgc cctgggcctg ctgctgatcg
     2461 tgttcatcgg ccactccatc gcgtcgggga tacacaggcc ggtggcgcaa ctggtcaacg
     2521 cctccaacga cgtggcgggt ggcgacctcg acgtgcagat acccgccacg cgccaggatg
     2581 agctgggcgt gctggcggct cgtttcaacg tgatggtgtc gggcctgcgg cattcgctgt
     2641 tcgtcaagga cctctttggc cgcttcgtgt cgccggaagt atcggccaag ctgctggcgg
     2701 gacagataga gcttgggggc gagcagcgag aggtaacggt gctgttcagc gacctgcgcg
     2761 atttcacccg cctctccgag cagtactccg ccccggccat cgtggacctc ctgaacgagc
     2821 atttcagcaa cgtaattcag gcggcgcagg agaacggcgg catcgtcaac aagttcggcg
     2881 gcgattcgac gctgatcatc ttcggcgcgc cggtggacat gcccgaccat gccgaccgcg
     2941 cgctcgccac tgccgtagca atgcaggccg ccctcggcag gctcaacgag cggcgcacga
     3001 aggaaggttg ggcaccgcta cgccagggga tcggtgtcaa tacgggtacg gttgtcgccg
     3061 ggcaaatagg ctccaaagcg cgcatggagt acacggtcat aggcgatgcg gtgaacctgg
     3121 cctcgcgcct ccaatcgatg accaaagatc tcaacggttg cgacatcgtt ttcagcgaga
     3181 acactctgct cgccctgtcg gacccgcccg aatggctagt ggaagacctg gggcctatcg
     3241 aggtgagggg caaatcgaag caggtgcggg catatggcct tgtaggacta gcagagggtg
     3301 atgtcgaggc cgcgcatgag ccggacaagt tggcggtact gccggagcgc gcgccggcgc
     3361 ttgagaatgt gcccgcagtc gtaggcgtgg ggcaggcacg ctgatgcagg ccggtcccgt
     3421 gcggccgcgg cgttcgacgg ccgggggcga tatgcgcgtg ctcctgctgg gcagcgggct
     3481 ggctttactt gctctatttg ccaccgcgtg gtgggcgctc cagcggccta cgcccccgtg
     3541 gctgaagggt gaggtgatag cctaccgggt cgctgattac ggccgggacg agaccgcccc
     3601 ggtgtacgtg ccgctcagcg ccgatatcgt gcaggccgtc atcaaggaca acgccacctt
     3661 tcaacgcgac ggtcgcgact tcgcaccgcc atcgcagagc gcggcgcaga gcgtggcctg
     3721 gttagctacc gaaacgcccg aacgtggagc actacctgac caggcggtgg gaggtacaac
     3781 cacgcccacg cgagtgccca caaacgccgc cactcccatg ccgcaagcct ccacccacac
     3841 agcgaccacc acgaggacgg taaccgcaat gagcacgccc gtcgctaccg gcactcctgc
     3901 cggcggccag ggtcctactc aggaggccaa cgcggaggtc acctggaccc cctctgccac
     3961 agccacgcgg accctgccgg gtgtgcctac agccacacag gaggccaccc cctcacctgc
     4021 cacgaagcgc ggggacgttg aggcgaccgc caccccgaag ccaacaagcg aagccgaccc
     4081 tggtaagaat aaggccaagc catcggctac cgccactgtg gggcgtacat cgagggtgcc
     4141 tacttccacc agcacgccgc ctcccgctcc ttccgacacg cccaccccga cggcagcgct
     4201 accgagcgac acaccgacac ccgcacctac agacacccca cagcccacac caagcgcaac
     4261 gccgagcctc acacctagcg ccacgccaag cgacacgccc acgatagcgc cgcccacgga
     4321 agaagccact gcaaccccgg acgctaagcc tactcacgag ccaaagccaa cagatgagcc
     4381 taagcccacc aaggagccga aagcgactga ggagcctagg cccaccaggg agccaaagcc
     4441 caccgatgag cctgcaccca caaaggagcc taagcctacc aaggagccga acggcagcgc
     4501 cttgccctaa gtggcccctc cgttttccct tagcttatgc cagtcgtgtg cccgtagcta
     4561 ctcatcgcgt gcctggcatc ctggcgttta gaaacctgtc tcgttagacg gtgctacacc
     4621 tggggtctgg tgagagaaaa cgtgcggtcg atggcggtat attcgtcacc ccccagccgg
     4681 gcgatggccc ctagctgtat cgcctgtacc aggccgttag cgcctagaag tccttcgcga
     4741 atatggtacc ggagcacctg ccccaggatc attgtgtaat gggtgtcggc gaccggcaca
     4801 atttgggtgt tgcggcattc cagcgcgacc ggggcctcca gtacgcgtgg gggcctcaca
     4861 tcggtcgaag gtacgcgggt caacccggcc aggtcaaact cgtcggcgtc gtagggccac
     4921 tccccggaag taaggttcag ccgagccgcc aatccttcgt tcgcgatgtg caccacgaac
     4981 tcacccgtct cctgtacgtt gcggagcgtg tccttgggcc tacccgtgcg ctgcccgacc
     5041 gacaccatga cgggaggggg agtaccgccc accacgttga aaaaggagaa gggcgcgaga
     5101 ttgtgggagc catcggcacc caacgtggag acccaggcga taggccgggg tacgacgatg
     5161 ctcacaagca gccggtaggc ctcgcgaggg tgaagctctc ccggtgtcag cgtaaccata
     5221 ggatattctg cgtccttctc catgtggtgt gctccggtcg taagtccgcg caacccgaag
     5281 gccggtcgcg ggctagcgat agtgactgtc ggggtcgagc tctttcctca gcaggtcaag
     5341 ctccgtgtca cctggtggcg gtgtttcagc cagcgtgctc gacactcgta ggtcccagcc
     5401 aacctcctgc tgcacctgtt ccaggctgac accggagtgg agtccgctga gttgcatctc
     5461 gtgctgtggg ttatcaaagt cgaagacggc aaggtccgtg acgacggtga cagggccttc
     5521 cccaggcatg ccggacgctc cccttgcttc cccgccggag agatggccgg ggctggtgcg
     5581 aaaatcgagc gcgctcacga aggagcggcg gctcagacgc atgatgtata tgaggcgtcg
     5641 ggcgttgatg gcaatttcgc aagccccgcc gcttccgggc aggcgtaccc tgggaccgtc
     5701 gtagtcgcct atgaccgtgg tgttgaggtt gccgaaacgg tccacctgcg cgccgcccag
     5761 cagggccacg tcgatgaggc cgccttgaag gtagaggcca aacaggtcgg gcatggacac
     5821 caccgagagc gccccactca ccagggcagg gtcgcctatt gagagcggct gccgggccgg
     5881 gcgagcgccg tacacccccg attcgtagat gagctgaagg tcgggcgctg ccgtgcgcag
     5941 ggcgaggttg caggccaggt tgggcagccc cacacccacg aacacggtgc gagcgccctg
     6001 caaggctcgt gcggctgcca ctatcatcat ttcggtaggg ctgtaggccg cgtcattcac
     6061 tagcgatagt ctccatagtc aacgggtggg ctgggggccg gaccggggcg cagtcgcgcc
     6121 agccgctcag gtcccagctt gcgcaggtat gtggcccggt ccggcacttc gtacacccac
     6181 tcccgcagcc attcagcggt gcgggcctca tcgcgggaga tttcgtccca ttccaggtag
     6241 aaggcgttgt cccggtcgta gtagccctga acgtaggagg ggtgggagcc aaacggctcg
     6301 tgcaccacgg catccacgat caggccgggg attaccgtgc ggttcgggtc ggcacgtatc
     6361 accgcctcgt ctacaatctc ttcgacgacc actatcaccc gcttggcggc gaacgctacc
     6421 tctttctgcg ctcccaggag gccccacatc tgcgtgttgc cctctacgtc ggcgcgctgg
     6481 gcctggagga ttgccacgtc ggggttgaga ggcggcacca cggccacttc accatctccg
     6541 taggggcttt ggacgctgcg aatgaggggg ttcaccaggg gcaagtcggt gcccgtgtag
     6601 ctgcggagcg ggaagaacgg cagcctgtac gccccggcca tgtaccgccc taccatcccg
     6661 tagtgcgagt actcctcgag ttccaggggc accggcttgc cctgttctat gcagcgtcgt
     6721 atcgcgagca gcggtcccac gcctgggttg cccaggtagc tgaacaccag cttgcgggcc
     6781 agtcccgctg ctacgagctg gtcgtagacg aggtctgggg tcatgcgggc cagggtgagg
     6841 ccgctatgcc cctggcggat gatttcgtgg gcggcggaga aagggatgga ggcggtgaaa
     6901 ccttccaggg caagcgtctg cccgctccga acgtaagtgg agatagcctc ccgca
//
LOCUS       DASRWZ010000230         3617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_909412, whole genome shotgun sequence.
ACCESSION   DASRWZ010000230 DASRWZ010000000
VERSION     DASRWZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909412"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1266)
                     /locus_tag="VFH60_03620"
     CDS             complement(31..1266)
                     /locus_tag="VFH60_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET6312901.1"
                     /translation="MLTEEKREQGPGQGQETTHTRTVAAPLPEVYRAFTHPVALRDWL
                     CDVAEVEPRTGGRFYLAWDNDYYTAGRYTELARNERVAFTWRGPSDPADSQVLVTLAQ
                     AAEGVTVTVTHAGVANEWAEGATASRLSWDAALENLQSILETGRDLRLIRRPMFGLNG
                     ADTLTAEIAAELGLPVEEGLRLTGLVEGLAAHNAGLRPNDVLVSLGGHSVRDYGSLGG
                     ALGLRRAGDQVPATFYRDGEQQTVTLELSARQLPAVPETKQELAESLRKDNAQVAQEL
                     DSLVEGVTDEEAEYRSSEDNWNAKEVLAHLIASEYDGHVTIGNFVADAPDDPAYYAND
                     LLRLRPLAAAHGSIEALAEELKRSQQVTADMLANMPAPASKRKRNVFRLGLGYVGFAE
                     HTRDHFNEIRVLVEAARAE"
     gene            complement(1515..2408)
                     /locus_tag="VFH60_03625"
     CDS             complement(1515..2408)
                     /locus_tag="VFH60_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6312902.1"
                     /translation="MVRLVVFPKCYMDELCVTRSMTVFEWIDLAATLPVDGVEMYDRF
                     LVETTPAYLRRVRDALDARGLAMPMMCYSPDFTVPDAGGRREEIDRQKRVMEVTAALG
                     GKYCRVLSGQRRPGVSRERGLAWVVEAVEALIPHALDLSLTLTLENHYKDNYWQHPEF
                     AQFSDVFLEILARVPSPALAVNYDPSNALLAGEDPIELLHKVKRRVATMHASDRTLKP
                     GHTLDDLRAQEDSTGYASILQHGEVGKGLNDFDAIFSTLREAGFDGWVSIEDGVNGLD
                     ELRRSAEFLREKIGRYWPRVV"
     gene            complement(2659..>3617)
                     /locus_tag="VFH60_03630"
     CDS             complement(2659..>3617)
                     /locus_tag="VFH60_03630"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6312903.1"
                     /translation="GAGEEVLRLKAVGICGSDVHGYTGTSGRRTPGVVMGHELAGVVS
                     EVGPGVEGLGQGDRVTVNPLLYCGECDYCVEGREQTCRNRRTIGVNMGRAGGLAEYVT
                     VPGRNCVPIDEGVSFAEGFMAEPLAVGLHAARLAAPTPGQPMLVLGGGAIGLCVLLAC
                     IQLGAGPVYVTDLLPHKLEMVERLGGRPLDAHGTDLAELARETTGGRGFPTIIDAVAD
                     STTIKQAVPALAPGGTLVLVGLARPLAEFALYDLVTQERVLRGSYAFSAAEFREAASW
                     INSRRVDVRPLLEASCTLAEATAMFDRRAAGEIDAVKIVVEL"
BASE COUNT          599 a         1310 c         1050 g          658 t
ORIGIN      
        1 cgttcttggc gtcttggcgg ttccgtctct ttactcagct ctggcggctt cgaccagcac
       61 ccggatttcg ttgaaatggt cgcgcgtgtg ttcggcgaag cccacgtagc cgaggccgag
      121 gcggaagacg ttgcgcttgc gcttggaagc aggcgcgggc atgttagcta gcatatccgc
      181 cgtgacctgc tggctgcgct tgagttcctc ggccagggcc tctatgctgc cgtgcgcggc
      241 tgccaggggg cgcaggcgca agagatcgtt ggcgtagtag gccgggtcgt caggggcgtc
      301 cgccacgaag ttgccgatgg tgacgtggcc gtcgtactcc gaggctatca ggtgcgccag
      361 tacctccttc gcgttccagt tgtcctcgct cgaacggtac tcagcctcct cgtccgtcac
      421 gccctcaacc aggctatcaa gctcctgggc cacctgcgcg ttgtccttgc gcaagctctc
      481 ggccaactcc tgcttagtct ccggcacagc gggcaattgc cgggccgaca gctctaacgt
      541 cacggtctgt tgctctccat cccggtagaa agtggcgggc acctggtcgc cggccctgcg
      601 cagaccgagc gccccgccca ggctgccgta gtcacgcacc gagtgcccgc ccaggctgac
      661 cagcacgtcg ttcggccgca agcccgcgtt gtgcgccgcg aggccctcca ccagccccgt
      721 caggcgcagg ccctcttcca ccggcaggcc caattcggct gctatctccg cggtcagcgt
      781 gtcggccccg ttcaggccga acataggccg ccgtatgagg cgcaggtccc ggcccgtctc
      841 caggatagat tgcaggttct ccagcgccgc gtcccaacta agcctggacg cggttgcgcc
      901 ctcggcccac tcgttagcaa ctccggcgtg cgtgacagtc acggtgacgc cttcagcagc
      961 ctgggcgagc gttaccagca cctggctgtc cgccgggtcg ctcggccccc gccacgtgaa
     1021 agccacccgc tcgttgcgcg caagctcggt gtacctgccg gccgtatagt agtcgttatc
     1081 ccacgccagg tagaagcgtc cgcccgtgcg cggctcgacc tcggccacgt cgcacagcca
     1141 gtcgcgcaaa gccaccgggt gggtgaatgc gcgatacacc tccggcagtg gggccgcgac
     1201 cgtccgcgtg tgggtcgttt cctgcccttg acctggccct tgctccctct tttcctcggt
     1261 cagcatcttg tcctccatct actaaaggat agtggtacat agccgcctgg gtaactctct
     1321 cttaccccaa tacggccgca tcgaaggcac aaccatgtgc cggtacggca gcgcagggta
     1381 cctttaagct agacagcaaa ccggccctcc cggttcactg caagccgcga tttggattca
     1441 gatacgggcg cgctcattcg ttagcatgga attgaggatg acaaggacca gcacggccct
     1501 ctggccgcaa ctcttcatac aaccctcggc cagtagcgcc cgatcttctc cctgaggaac
     1561 tcggcggagc ggcgcaattc gtccagccca ttgacgccat cctctatcga aacccagccg
     1621 tcgaaaccgg cctcccgcaa cgtcgaaaag atggcgtcga aatcgttcaa gcccttgccg
     1681 acctccccgt gctgcaaaat agaggcgtag cccgtggagt cctcctgcgc ccgcaggtcg
     1741 tccagcgtat ggcccggctt gagcgtccgg tcgctcgcgt gcattgtggc cacccggcgc
     1801 ttgactttgt gtaacaactc gatagggtct tcccctgcca gtagggcgtt cgaggggtcg
     1861 tagttcaccg ccagggcggg cgagggcacg cgggccagta tctccaggaa cacgtcgctg
     1921 aactgggcga actccgggtg ctgccagtag ttgtctttgt aatggttctc cagcgtcagc
     1981 gtgaggctca ggtccagcgc gtgggggatg agggcctcta ccgcctccac cacccaggct
     2041 agcccccgtt cgcggctcac ccccggcctg cgctgccccg acagcacccg gcagtactta
     2101 ccgcccagcg ccgccgtgac ttccatcacc cgcttctgcc tgtcgatttc ctcgcgccga
     2161 ccaccggcat ccgggaccgt gaagtccggc gagtagcaca tcatcggcat cgccagcccc
     2221 ctcgcatcga gcgcgtcacg cacccgccgc aagtaggcag gtgtcgtctc caccagaaag
     2281 cggtcgtaca tctccacgcc gtccacaggc agggtcgcgg ccaagtctat ccattcgaac
     2341 acggtcatac tgcgcgtaac gcacagctcg tccatgtagc acttggggaa caccaccaac
     2401 cttaccatgc cgccctcacc cctctgtcat cctgagcgta gcgaaggatc taagctgctc
     2461 cttcagccgc caaattggac ctacagcacc atgttcgtaa tgttcaacat ggtccccgta
     2521 ccagcttctt gagatccttc actgcgttca ggatgacaga gggggactcg gtcctgttca
     2581 ccctgcaatt tccatccccg gtttccaata gaaccggcat ccacaggtaa ggggcgtctg
     2641 cgagctatct gcatccgcct acaactccac aactatcttc accgcatcga tctcccccgc
     2701 ggccctgcgg tcgaacatag cggtggcctc agcgagggta caactggcct ccaggagggg
     2761 gcgcacgtcc acgcggcggc tgttgatcca gcttgcggct tcgcggaact cggcggccga
     2821 gaaggcatag gagcctctca ggacgcgctc ctgggtcaca aggtcgtaca acgcgaactc
     2881 ggccaggggc ctggcgagtc ccaccagcac cagcgtgccg cccggggcca gcgcgggcac
     2941 cgcctgcttg atagtagtcg agtcggccac cgcgtctatg atcgtgggga agccgcgtcc
     3001 gcccgtcgtc tcccgcgcca gctctgccag gtcggtgccg tgcgcatcga gcggcctgcc
     3061 acccaggcgc tccaccatct ccagcttgtg aggcagcagg tcggtcacat ataccggccc
     3121 cgcccctagc tggatgcacg cgagcaagac gcacaggccg attgcgccgc cacccagcac
     3181 cagcatgggc tgtccgggcg ttggcgctgc gagcctcgcg gcgtggaggc ccaccgccag
     3241 tggctcggcc atgaagcctt cagcgaagct cacgccctcg tctatcggca cgcagttgcg
     3301 gccggggacc gtcacgtact ccgcgagacc cccggccctg cccatgttca caccgatcgt
     3361 ccggcggttg cggcaggtct gctctctgcc ctccacacaa tagtcgcact ccccacagta
     3421 caacagcggg ttgacggtca cgcggtcgcc ctgccccaac ccctcgaccc ccggcccaac
     3481 ttccgacacc acgcccgcca gttcgtgccc catcaccacg cccggcgtcc gccgccccga
     3541 ggtgcccgtg tagccatgca cgtcgctgcc gcagatgccg acagccttca gccgcagcac
     3601 ctcttcgccc gcgccgg
//
LOCUS       DASRWZ010000231         1545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_498076, whole genome shotgun sequence.
ACCESSION   DASRWZ010000231 DASRWZ010000000
VERSION     DASRWZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498076"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..715
                     /gene="surE"
                     /locus_tag="VFH60_03635"
                     /pseudo
     CDS             2..715
                     /gene="surE"
                     /locus_tag="VFH60_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
     gene            717..1364
                     /gene="thpR"
                     /locus_tag="VFH60_03640"
     CDS             717..1364
                     /gene="thpR"
                     /locus_tag="VFH60_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET6312904.1"
                     /translation="MTSDANTNTGERDSARLFVAIELPQDILRVIEGIQAQTRENLGE
                     AASLLRWSRPESIHITLQFLGETPTARIAAITEALQQACAGAGPFSLEVGGLGAFPNV
                     RRPRVVWVGLGGDTGAAVGLTTSIQEALAPLGFKPDKPFSPHMTIARVREGNPVGKLA
                     PLSRVLSMTGIVLPEETRFQVEGVSLMQSFMQSGGSVYKQLAHVALVGGGEAAAG"
BASE COUNT          301 a          471 c          511 g          262 t
ORIGIN      
        1 cttgctggcc ctgcacaccg cgctggcgcc cgaccacgaa gttgtcatca tcgcccccga
       61 ccgcaactgg tcgatttcgg gccacaaccg cacgatggac cgcccaatca gggtgaacga
      121 ggtgacgttg cgcgacggca cccaggcgta gtccagcgac ggctcccccg ccgactgcgt
      181 ggccctggcc gcgctgggct tcctgcccga aaagccggag ctggtcatat cgggcatcaa
      241 caacggctac aacctggccg acgacatcac atattccggc acggtggccg ccgcgatgga
      301 gggcatcatc tcggagatac ctgctatcgc ggtctcggcg gacacgcacc cggtgtggca
      361 gatagaggtc gcggcgggcg cggtggctcg gctggtgcag caggtggcta agcagggcct
      421 tgacaacgac atcttgctca acgtgaacgt gccaagcctc acctacgagg agatcaaggg
      481 cgtccagata acccgcctcg gcaaacgggt ctaccaggac aagctgatag agcggctgga
      541 cccgcgaggc cgcaagtact actggatagg cggcacggag gtgctcagca cgcacatgga
      601 gggcaccgac gccacagctt gcgagcatgg ctacgtctcc gtcacgccca tacacctcga
      661 cttcaccaac cacgcgctgt tggacaggtt gcggtcgtgg gacttgaggt tctaaagtga
      721 catcagatgc caataccaac accggggaaa gggactccgc gcgcctattc gtggcgatag
      781 agttgccaca agacatactg cgggtaatcg agggcatcca ggcgcagaca cgcgagaacc
      841 tgggcgaagc ggcgagcctg ctgcggtggt cgcgccccga aagcatacac atcaccttgc
      901 agttcctggg agagacgcca accgcgcgca tagctgcgat aactgaggcg ttgcagcagg
      961 cgtgcgcggg ggcggggcca ttctcgctcg aagtgggcgg gctgggcgcg ttccccaatg
     1021 tgaggcgacc gcgcgttgtg tgggtgggcc tcggtgggga tacgggagcc gctgtcggcc
     1081 tcacgacctc gatccaggag gcgcttgccc ctcttggctt caagccggac aagcccttca
     1141 gcccacacat gaccatcgcc agggtgcgcg aaggcaaccc ggtcgggaag ctcgcacccc
     1201 tctcgcgcgt gctatcgatg acgggcatcg tgcttcctga ggagacgaga ttccaggtag
     1261 agggcgtaag cctcatgcag agcttcatgc agtcgggagg ctcggtctac aagcagttgg
     1321 cgcatgtcgc gttggtgggc ggtggggagg cggcggccgg ctgaggcagc ggtgtccgcg
     1381 catcgccaaa ggtcctcttg caggctcttc tcttttgtcc cataatagca acaaatatcg
     1441 gcagccaggg cgtttctcct atattgaccg ggagacctgc tgttcgcgta ttagggtggg
     1501 gacgcgaacg cagagctatg ataaggctgt ggattgccgc gtgcg
//
LOCUS       DASRWZ010000232         5264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1183567, whole genome shotgun sequence.
ACCESSION   DASRWZ010000232 DASRWZ010000000
VERSION     DASRWZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183567"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFH60_03645"
     CDS             complement(<1..478)
                     /locus_tag="VFH60_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqG family protein"
                     /protein_id="HET6312905.1"
                     /translation="MTPDISALREQIDASLQRVAAELQALARNSIRIKTIPVTDEALL
                     PPGTSKFGGLPDLPADVAWPEWNGEPLAFIAQFRLEEVAPYDLDRLLPATGMLYFFYE
                     AQQSTWGDLPGDKGSARVIYYDGDAARLRGTPFPETLHEWGRDYPPCRLELSSEASM"
     gene            complement(498..3230)
                     /locus_tag="VFH60_03650"
     CDS             complement(498..3230)
                     /locus_tag="VFH60_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6312906.1"
                     /translation="MADSKANGASKGKRARHGTQLSLDFSSPEAQDEPQLQLVEAQTE
                     VPQEPAWLSEAPTPQPEMDGGDVSAGAPQKDLPAPARFNHTPDANLMERILTGLNKEQ
                     REAVTTTQGALLIIAGPGSGKTRVLTHRIAYMIEADGVWPHRICAVTFTNKAAFEMKS
                     RLEKLIGPRVKDLTVGTFHSLGVRILRQHADLLGYSRDFAIYDDEDQQKIVKEAMSYV
                     NLDDKQYSPRAILSRISSAKAAFASPEQASKLAETYFDELAARVYRRYQELLIKNQAM
                     DFDDLIMLTIELFTTQPEVLRKYQERYLHVLEDEYQDTNHSQYIMVKMLSGIHGNLCV
                     VGDEDQSVYSFRSADIRNILEFERDFPNAKTIILGQNYRSTQTILKVADSVIKQNTQR
                     KSEKHLWTENAAGLPITLFEAYDEAEEASYVAREVRRLVGSGYSYKDVAVTYRTNAQS
                     REIEQACMLYQIPYQLVGGVRFYSRKEVKDVMSILRVVHNPHSSVDFARMVQNTPLGK
                     GIGAKTVAELEAYATKLGMSYYEALHTAVKQSRRDKAEQMQAGTPILNVPTSKFAVML
                     TMLEEIIASRPDLPVVGLLDLLLEKTRYKDFLEEGAGQEGLERWQNVLELRTVAENYA
                     DLPTSEQLGRFLEDVALLSDVDSLKEEKDAITLITLHAAKGLEFPIVFIIGMDEGILP
                     HSRSLEDENQMEEERRLAYVGITRAKERLYMLYAFRRTLYGMTQMNGPSRFIADVPAD
                     LVTGRDPGTAFNPIIQGNKRNTVPTEGFSADDILGGKTSIYGKSKDRDRPTFGSGIPR
                     ATSGSGGRGTYGPSGRPTSPRTEGRRENKRQEQNTRLNITSAADLRDRAQNGSAAPTG
                     ERGFKVGERVTHHTFGEGQVLAVKPSANDEEVTVMFKKAGTKRLLASAARLEKL"
     gene            3879..4595
                     /locus_tag="VFH60_03655"
     CDS             3879..4595
                     /locus_tag="VFH60_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312907.1"
                     /translation="MSTQMQTCSVCGGQVPVGSRFCQNCGHSMPQAEDSSARVQGFTP
                     PQGSPTTNVDPQYNPNNAPYSPQPNTASLQQGQYGEVPRQHPQPQYSPPAQGYAPPPP
                     SLYGQQPPSYQQTAMQPQGYAPPQGYSEAPLYAAPAYGQYAAQPQRDPVLALLLELIG
                     YVGVLGIGHIYAGRTSRGVGLLVGWLLYGVIIFFLFITIIGSPIACLMLLLTPIVPIL
                     SGLWIKNDLDKERAAHAPRY"
     gene            4694..5029
                     /locus_tag="VFH60_03660"
     CDS             4694..5029
                     /locus_tag="VFH60_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HET6312908.1"
                     /translation="MNGNETNGNSNGGTQSNETYGQGGAGGTATASGTRTEEFKLSGE
                     DIIGKVREIIEEGNARRIILRNAEGHNLIEIPLTVGLVGAALLPVYAALGAAVALAAN
                     LMSVVEKRA"
BASE COUNT          915 a         1568 c         1675 g         1106 t
ORIGIN      
        1 gcatacttgc ctcagaggat aactctaacc ggcacggcgg gtagtctcga ccccattcgt
       61 gcaacgtttc gggaaaggga gtgcctctga gccgcgcggc atcgccgtcg tagtatatga
      121 ccctggcgct acctttgtcc cctggaaggt ctccccaggt gctttgttgg gcttcgtaga
      181 agaagtaaag catacccgtc gcgggcagaa ggcgatcgag gtcgtaaggg gcaacttctt
      241 ccagcctgaa ctgcgcgatg aacgcaagcg gttcaccatt ccattcaggc caggcgacat
      301 ccgcaggtag gtccggcaga cctccgaatt tcgacgtgcc cggcggcaac aatgcctcgt
      361 ccgtgacagg tatagtcttg atacgaatag agttgcgggc cagcgcctgc aactccgctg
      421 ctaccctctg caaactggcg tctatctgct cgcgtagggc gctgatgtcg ggtgtcacgc
      481 gatacctccg cgctgctcta cagcttctcc aaccgagccg cgcttgccag gagcctcttg
      541 gtgccggcct tcttgaacat gaccgtaacc tcttcgtcgt tggcgctagg cttgaccgcg
      601 agcacctggc cctctccgaa ggtgtggtgg gtcacgcgct cgcccacctt gaagccgcgc
      661 tctccggtgg gggctgcgga gccgttctgg gcgcggtcgc gcaggtcggc ggcggaggtg
      721 atgttgaggc gggtgttctg ctcctggcgc ttgttttccc ggcggccctc ggtgcggggc
      781 gaggtggggc ggccgctcgg gccataggta ccccgaccgc cggagcctga ggtggcgcgg
      841 ggtatgccgg agccgaacgt gggcctgtcg cggtctttgc tcttgccgta gatgctcgtt
      901 ttgccgccga ggatgtcgtc ggcgctgaag ccctcagtgg gcacggtgtt gcgcttgttg
      961 ccctggatta tggggttgaa ggcggtgccg gggtcgcggc ccgtgacgag gtcggcgggc
     1021 acgtcggcga tgaagcggga ggggccgttc atctgggtca tgccgtagag ggtgcggcgg
     1081 aaggcgtaga gcatgtaaag ccgctccttg gcccgcgtga tgcccacgta ggccaggcgg
     1141 cgctcctcct ccatctggtt ctcgtcctcc agcgaccggc tgtgcggcag tatcccctcg
     1201 tccatgccga tgatgaagac gatggggaac tcaagcccct tggcggcgtg cagggtgatg
     1261 agggtaatgg cgtccttctc ctccttgagc gagtccacgt cggagaggag ggccacgtcc
     1321 tccaggaagc ggcctagctg ctcggaggta ggcaggtcgg cgtagttctc ggccacggtg
     1381 cgcagctcaa gcacgttctg ccagcgctcc agcccttcct gcccggcccc ttcctccagg
     1441 aagtccttgt agcgggtctt ctcaagcaaa aggtccagca gccccaccac tggcaggtcg
     1501 ggacgcgagg cgataatctc ctctaacatg gtgagcatga cggcgaactt ggaggtgggc
     1561 acgttcaata tgggggtgcc cgcctgcatc tgctcggcct tgtcgcgcct gctctgcttt
     1621 acggcagtgt gcagcgcctc gtagtacgac atgcccagct tggtcgcgta ggcttccagc
     1681 tcggctacgg tcttggcccc gatgcccttg ccgagggggg tgttctgcac catgcgggcg
     1741 aaatcgacgc tgctgtgggg gttgtgcacc acgcgcagga tggacattac gtccttgact
     1801 tccttgcgcg agtagaagcg caccccgccc acgagttggt acggtatctg gtagagcatg
     1861 cacgcctgct cgatttctcg cgactgggcg ttggtacggt aagtaacggc tacgtccttg
     1921 tacgagtagc cgctgcccac gagcctgcgt acctcgcggg ccacgtagct ggcttcctcc
     1981 gcctcgtcgt aagcctcgaa cagcgtaatg ggcaggccgg cggcgttctc ggtccacagg
     2041 tgtttttcgc tcttgcgctg ggtgttctgc ttgatgacgc tgtcggctac cttgaggatg
     2101 gtctgcgtgg agcggtagtt ctggcccagg atgatcgtct tggcgttggg gaagtcgcgc
     2161 tcgaattcga ggatgttgcg gatgtcggcc gagcggaagc tgtacacact ttgatcctcg
     2221 tcgcccacaa cgcacagatt gccgtgtatg cccgacagca tcttgaccat gatgtactgc
     2281 gagtggttgg tgtcctggta ctcgtcctcg agcacgtgca ggtagcgctc ctggtacttg
     2341 cgtagcactt cgggctgggt ggtaaagagt tcaatggtga gcatgataag gtcgtcaaag
     2401 tccatcgcct ggtttttaat tagcagttcc tgatagcggc gatagacgcg ggcggccaat
     2461 tcatcgaagt aggtctcggc cagtttgctc gcctgttcgg ggctggcgaa agcggccttc
     2521 gcggagctga tgcgcgacag gatggcgcgg ggcgagtact gcttgtcgtc gaggttgacg
     2581 tagctcatcg cctccttgac gatcttttgc tggtcctcgt cgtcgtaaat tgcgaagtcg
     2641 cggctgtagc cgagcaggtc ggcgtgctgg cgcaggatgc ggacgccgag ggagtggaag
     2701 gtgcccacgg tgaggtcttt cacgcggggg ccgatcagct tctccaggcg gctcttcatc
     2761 tcgaaggcgg ccttgttggt gaacgtcacg gcgcagatgc ggtggggcca cacgccgtcg
     2821 gcctcgatca tgtaggcaat gcggtgggtg agcacgcggg tcttgccgct gcccggcccc
     2881 gcgatgatga gcagcgcccc ttgggtggtg gtgactgcct cgcgctgctc tttgttcagc
     2941 ccggtgagga tgcgctccat gaggttggca tcgggggtgt ggttgaaccg cgcgggggcg
     3001 ggaaggtctt tctggggcgc acctgcactc acatccccac cgtccatctc aggctgaggt
     3061 gtgggagctt ccgaaagcca ggcgggttcc tggggcacct ctgtttgggc ttccaccagt
     3121 tgtagctgcg gctcgtcctg ggcctcgggc gaagagaaat cgaggctcaa ctgggtgcca
     3181 tgccgtgcgc gcttgccctt agaggctccg ttcgccttgc tgtctgccaa cgtggaagct
     3241 cctgtctagg aatgagcggg tgccggtgac cttgagtgct tgacggaggc cctggctgcc
     3301 taatatcctg gggggtagta catatattct aacatgtgtg cccggctcgc ggcaaagcta
     3361 tccacacgga aaatcggacc tatgatgtgt acaactagac ccggtgcata acctcactgt
     3421 cgcatctcat tggaccaact ccccctgtca tcctgagccc ttcggctacg ctcagggtaa
     3481 actccgcgaa ggatcaggcg ggtaggctag aggctacaac gctggggatg ctggcggtaa
     3541 gtggcacatt gttgcaacaa tagtgagaat caaacacggg cggctgatcc ttcgctacgc
     3601 tcaggatgac aggggggagt tggtcctggc tgtctggtcg cgtaggtatt agtcccaggg
     3661 aacgctgcat cgtgccccgc aaccccaaac catctcccct ctgcgcgttc gttcgtgctt
     3721 ttcgcccctt cggctgcgct cagggcgtgc tttgcggttt cgtctttcct cgtttgctct
     3781 catgtagttc tgcaacttag ctgcgaatag tgcaactttg ccggggggtt tcgcgtagtt
     3841 attttcggct aagccaattt tatggagatg taagacttat gagcacccaa atgcaaacat
     3901 gctcggtgtg cgggggccag gtgccggtag ggtcgcggtt ctgccagaac tgcgggcatt
     3961 ccatgccaca ggcggaagac tcctcggccc gcgtgcaggg tttcaccccg ccgcagggtt
     4021 cgcccaccac caacgtggac ccgcaatata atcccaacaa cgcgccctac tcgccccagc
     4081 ccaatacggc gtctctacag caggggcagt atggcgaggt acctcgtcag catccgcagc
     4141 cacagtacag cccaccggcc cagggctacg caccgccacc cccgtcgctc tacggccagc
     4201 agccgcccag ctaccagcaa acggccatgc agccccaggg gtacgcacca ccgcagggct
     4261 actcggaggc cccgctctac gccgcccccg cctacgggca gtacgccgcc caaccgcaac
     4321 gcgacccggt cctggcgctg ctgctggagc ttatcggcta cgtgggcgtg ctcggtatag
     4381 ggcacatcta cgcggggcgc acctcgcgcg gcgtgggatt gctggtaggc tggctccttt
     4441 acggggtgat catcttcttc ctgttcatta cgatcatcgg ctcgcctatc gcttgcttga
     4501 tgttgctcct cacacccatc gtcccgatac tctccgggct gtggatcaag aacgacctcg
     4561 acaaggagcg cgccgcacac gcacccaggt actagcgcga gaggacttat caaccgcagg
     4621 accggccacc cattcccgtt tacataacgc cggtgacaag gccgggcaca acacaactca
     4681 gaaggagaag caaatgaacg gcaacgaaac taacggtaac agcaacggcg gcactcagtc
     4741 gaacgagact tacggccagg gcggcgcagg cggcaccgcc accgccagcg gcacccgcac
     4801 cgaggagttc aagctcagcg gcgaggacat catcggcaag gttcgagaga tcatcgaaga
     4861 aggcaacgcc cgccgcatca tcctgcgcaa cgccgagggc cataacctca tcgagatccc
     4921 cctgaccgta ggcctggtag gcgctgccct cctgccggtg tacgccgctc tcggcgcggc
     4981 agtagccctc gcggccaacc tcatgagtgt ggtcgagaag agagcgtaag aggaatttag
     5041 gaattaggat ttcggattgc ggatttgggt tcgcgggccg aggtctgagt gttattggca
     5101 caatcagaga agggcgactc ggtaaactga gtcgcccttt ttgcatccta ccaacgaatc
     5161 cgaagtccga aatcctaatt cctaaatttc ttcgacttct gcctgtaaga atttgcgatc
     5221 ttcaggcgtt ggaataggtg agacgaagtg aacagttctt ttgc
//
LOCUS       DASRWZ010000233         5249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1046353, whole genome shotgun sequence.
ACCESSION   DASRWZ010000233 DASRWZ010000000
VERSION     DASRWZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046353"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /gene="guaA"
                     /locus_tag="VFH60_03665"
     CDS             complement(<1..988)
                     /gene="guaA"
                     /locus_tag="VFH60_03665"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET6312909.1"
                     /translation="MDKGQLEIDELLLEAEEIVAEEAGRPEVEGPLVSAPRPAQQHDT
                     VVVLDFGSQYSQLIVRRVRELGVYAELLPCDAPQAERDRLAPAAFILSGGPASVYEPG
                     APQLPRYVLGDERPVLGICYGLHLLTRAYGGEVEGSTHREFGPATIRRVAESPLFEGL
                     GDSLPVWMSHGDRVMEPPAGFELLAVSDNAPVAAMGRGPVLGIQFHPEVIHTPQGKEE
                     LSNFLFGVAGLRPTWNSASFIETAIDQIREAVGEGRVLCGLSGGVDSSVAAALVQRAI
                     GDRLVCLFVDNGLLRQNEAAEVVETFGRNMHMNLVAVDAADRFLDRLEGIDDP"
     gene            complement(1045..1851)
                     /locus_tag="VFH60_03670"
     CDS             complement(1045..1851)
                     /locus_tag="VFH60_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6312910.1"
                     /translation="MIATLTRTRQPGSSTHGADEHNIEVEGVSLRYLEAGAEHGGIPI
                     LMLHGLQGGADLWLPHPLPALAERFHVIAPDLPGFGDSGMLSSYGMGAYTTVMLAFMD
                     ALGHARFNLIGHSLGAQIAITMAAMQPERVNRLLVADGSGLPQGGPRWLNPVKLLTDA
                     SSWHFKLYPKVFRLARKSRAFREGLQALQRDYVTDRLRDVTMPTLVVWGSRDRVAPLE
                     HGAFLAKHIPHARLAIIRGAGHMPFYERPAQFMKLAEAFFSAGRGGEAAG"
     gene            complement(1883..2695)
                     /locus_tag="VFH60_03675"
     CDS             complement(1883..2695)
                     /locus_tag="VFH60_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6312911.1"
                     /translation="MKSTSTSARTARLRLPLSRSRARHDRYARVDGVRVRYVEAGAEH
                     GGVPLVIVHGYNGSCDYWYPQTVLGLAERRHVIAVDLPGNGLSGKLAAHNMETLSDFI
                     VRFVETLGYAQADLMGHSMGGLLAVAAANARPERVRSLVLVDSAGLPDLVKHLWLVPI
                     VALADSSMRQWRFYPTFIKIGLRARTARECLRMVSKVSMRELLAGLDLPVFILWGEND
                     RVVPVEHGYYMAEHIAGAKLAVVAGVGHMPFYEKPAECNRLILDFIEGLERR"
     gene            complement(2655..3122)
                     /locus_tag="VFH60_03680"
     CDS             complement(2655..3122)
                     /locus_tag="VFH60_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HET6312912.1"
                     /translation="MRTEGSTDGTGVGLRLTLPLPPGVNNQYATVNGRRVLSAEARAY
                     KQAVKRQVDELGFSGQVSPAMLATLRGSYLSLFIDFYFASPLKRDLDGGLKVTQDALC
                     EALGVNDNRVVDIHLVKRIDPRNPRIEVQLEAIAPDEWKFDEEYLYLGADGPA"
     gene            complement(3182..3766)
                     /locus_tag="VFH60_03685"
     CDS             complement(3182..3766)
                     /locus_tag="VFH60_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312913.1"
                     /translation="MKGGIVLSYIVGQYLSRNTGSVALLGACYMFLAGVLIMVAPVLA
                     GQSGVPVDSLGGVLIAIVGTLVLGLVAAVIGGLVAMLDGLVCGLASGAVIGILVQWVL
                     PRTLPPARYQAVFRAVGVITSLIASLATLSLLLTWMFSGFTTSNVVISSFSLVVLPSL
                     IAAIPNWWIGARIARWYAVKVREPDGGSAEEMAE"
     gene            complement(3801..5087)
                     /locus_tag="VFH60_03690"
     CDS             complement(3801..5087)
                     /locus_tag="VFH60_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6312914.1"
                     /translation="MFPVEPPETPAPPGGTAERPRHDPVAALRFRDFRLLIMGSFLAV
                     FAEQMLMVAVGWELYERTRDPLALGLVGLVEIVPVLLLALPAGQVADRRERKWVVVAA
                     MSGLAACAFGLVVLSLNTGPLPAIYALLFGIGVARSFQSPAFSAMSAQVVPSTHYGSA
                     AAWSSGAWQTAAIVGPALGGLLIALWGGAAGVYATSGGLLVLVALLFCLLRPRPVDRV
                     TEPINLTSLLAGVRFIWRTPLILTAITLDMFAVLLGGATALLPVFARDILEVGAGGLG
                     WLRAAPAAGALVAALTMALMPPLRYAGRTLLVVVAGFGVATVVFGLSRDFALSLSMLA
                     LLGALDSVSVVIRSTLVLTLTPDALRGRVNAVHYVFVGISNELGAFESGVAAALIGTV
                     GAVVAGGLGTLLVVVAVAVMAPELRRLGRLDTHHTS"
BASE COUNT         1030 a         1870 c         1560 g          789 t
ORIGIN      
        1 ccgggtcgtc tatcccctcc agccggtcga ggaacctgtc agcggcatcc acggccacga
       61 ggttcatgtg catgttacgg ccaaaagtct ccaccacctc cgccgcttcg ttctgccgca
      121 agaggccgtt gtccacgaac aggcacacaa gccggtcgcc aatcgcccgc tgcacgagcg
      181 ccgccgccac cgacgaatcg accccgccgc tcaggccgca cagcacccgc ccctcgccta
      241 ccgcctcgcg tatctggtct atcgccgtct cgatgaagga ggctgagttc caggtggggc
      301 gcagccccgc caccccgaac aggaagttcg acagctcctc cttgccctgc ggcgtgtgta
      361 tcacctccgg gtggaactgt atgccgagca caggcccccg ccccatcgcc gctaccgggg
      421 cgttgtccga gacggccagc agctcgaacc cggcgggcgg ctccatcacc cggtcgccgt
      481 ggctcatcca gacgggcagc gagtccccca gcccctcgaa caggggggac tcggccaccc
      541 gtcggatcgt ggcggggccg aactcgcggt gcgtcgagcc ttcgacctcg cccccatagg
      601 cgcgggtgag cagatgcagg ccgtagcaga tgccgagcac cggcctttcg tcgccaagca
      661 cgtaccgggg tagttgcggc gctcccggtt cgtacacgct ggccgggccg cccgacagga
      721 tgaacgcggc gggtgcaagc cggtctctct cggcttgcgg ggcgtcgcag ggcagcagtt
      781 ccgcgtatac gccaagctcg cgcacccgcc gcacgatcag ttgcgagtac tgcgacccga
      841 aatcgagcac cactacggtg tcatgctgct gcgccgggcg tggtgcgctt acgagcggcc
      901 cttccacttc cgggcgtccc gcttcctccg ctactatctc ctcggcctca agcaatagct
      961 catcgatctc caactgcccc ttgtccaagg tagacctcca tattgactga gagcgaacga
     1021 tacacgctac ttctgcctct gccgctaccc ggcggcctcc ccgccccggc cggcgctgaa
     1081 gaacgcctcg gccagcttca tgaactgtgc gggcctctcg taaaaaggca tgtgcccggc
     1141 gcccctgata atcgcaagcc gagcgtgcgg gatgtgcttg gcgagaaaag cgccatgctc
     1201 cagcggggcc acgcggtcgc gcgaacccca cacgacgagc gtgggcatcg tcacgtcgcg
     1261 caggcggtcg gtgacgtagt cgcgttgcag cgcttgcagc ccctcgcgaa aggccctcga
     1321 cttgcgggcc agccggaaca ccttggggta gagcttgaaa tgccaggagc ttgcatcggt
     1381 cagcagcttg acggggttga gccagcgcgg gccaccttgc ggcaggccgg agccatcggc
     1441 aaccagcagc ctgttcacgc gctccggctg catggccgcc atcgtgatgg ctatctgcgc
     1501 ccccagcgag tgccctatca ggttgaagcg ggcatgccca agcgcatcca tgaaggccag
     1561 cattaccgtt gtgtaagcgc ccatcccata ggaggagagc atgccagaat cgccaaagcc
     1621 gggcaagtcg ggggcaatta cgtggaagcg ctcggccagc gcggggagcg ggtgcggcag
     1681 ccacaggtcg gccccaccct gcaagccgtg cagcatcagg atgggtatgc ccccatgctc
     1741 ggcccccgct tcgaggtagc gcagggagac cccttccacc tcgatattgt gctcgtcagc
     1801 accatgcgtg gaggagcctg gttgtcttgt ccgggtcagt gtggcaatca tacctgtacg
     1861 cagcgcagat tatgtccgct tatcatcgcc gctcgagccc ctcgatgaaa tccaggatca
     1921 gccggttgca ctcggcgggc ttctcgtaga aaggcatgtg ccccacgccc gccacgacgg
     1981 ccagtttggc ccccgcgatg tgctcggcca tgtagtagcc gtgctctacc ggcacaaccc
     2041 ggtcgttctc gccccacagg atgaacaccg gcaaatcgag ccctgccagc agctcgcgca
     2101 tgctgacctt gctcaccatt cgcaggcact cgcgcgcggt ccgcgcccgc agcccgatct
     2161 tgatgaaggt cgggtagaag cgccactgcc tcatgctgga atcggccagg gccactatag
     2221 gcaccagcca caggtgcttc accaggtcgg gcagccccgc gctgtccacc aggacgaggc
     2281 tgcggaccct ctcggggcgt gcgttcgcgg cggccacggc cagcaagcct cccattgagt
     2341 gccccatgag gtcggcctgc gcgtagccga gcgtttccac gaagcgcact atgaagtcgc
     2401 tcagcgtctc catgttgtgg gccgcgagct tgcccgaaag gccattgccc ggcaggtcca
     2461 ccgcgataac gtgcctgcgc tcggccagcc ccagcacggt ctgcgggtac cagtagtcac
     2521 agctaccgtt gtagccgtgc actatcacca gcggcacccc gccgtgctcc gcgcccgcct
     2581 ccacgtagcg cacccgcacc ccgtccaccc gcgcatacct gtcgtgccgg gcacgcgagc
     2641 gggacagcgg gagcctaagc cgggccgtcc gcgccgaggt agaggtactc ttcatcgaac
     2701 ttccattcgt cgggcgcaat agcctccaat tgcacttcaa tacgtgggtt gcgggggtcg
     2761 atgcgcttca ccaggtggat atccaccacg cggttgtcgt tcacacccag cgcctcgcac
     2821 aacgcatcct gcgtcacctt gaggccgccg tcgaggtcgc gtttgagggg cgaagcaaag
     2881 tagaaatcaa tgaacagcga caggtagctg ccgcgtagcg tggcgagcat cgcgggagag
     2941 acctggcccg agaagcccaa ctcgtcaacc tgccgcttca ccgcctgctt gtaggcccgc
     3001 gcttcggcgc tgagcacccg cctgccgttc acggtggcgt actggttgtt cacccccgga
     3061 ggcagcggca gcgtcaaccg caggcccacg ccggtgccgt ccgtgcttcc ctccgtcctc
     3121 atggagcgat tataacacaa tgcccggacg gttttcgcag catcgaaacc ccaggacccc
     3181 tctactccgc catttcctcg gcgctcccgc catccggttc gcgcactttg acggcatacc
     3241 agcgtgcgat acgcgcacct atccaccaat tagggattgc ggcaattaaa gaaggcaaga
     3301 caaccaggct gaacgaggaa atgacgacat tcgaagtagt aaatccactg aacatccacg
     3361 tcagtaacag ggacagagtc gcaagacttg cgatcaaact cgttatcacc ccgaccgctc
     3421 gaaacaccgc ttgatatctg gccggaggca gcgtcctggg caacacccat tgcaccagga
     3481 taccgataac ggccccactc gcaaggccgc acacgaggcc atccagcatg gcgacaagcc
     3541 cgcctatcac ggctgccact aagccaagca caagagtgcc aactatggcg atcaggactc
     3601 ctccaagaga atccacgggt actccggatt gtcctgccag aacgggcgcg accataatca
     3661 gtacacccgc gaggaacata tagcaagcgc caagaagggc cacggaaccc gtgtttcgag
     3721 aaaggtactg gccgacgatg tacgacagca ctatgccacc cttcatagca gcgtcccttc
     3781 ccatgtgccc atgccaatcc tcacgatgtg tggtgcgtat ccaaccgccc cagccgccgc
     3841 agctcgggtg ccatcaccgc caccgccact accaccagca acgtgcccag gccccctgcc
     3901 accaccgcgc ccactgtgcc aatcagggct gccgccacgc ccgactcgaa cgcccctagc
     3961 tcgttcgaga tgccgacgaa cacgtagtgc accgcgttca cgcgcccgcg tagcgcatcg
     4021 ggggtgagcg tcagaaccag cgtgctgcgt atcaccacgc tcacgctgtc gagggcaccc
     4081 agcagcgcga gcatcgacag ggagagcgcg aagtcgcggg acagcccgaa caccaccgtc
     4141 gccaccccaa accccgccac caccaccagc aaggtgcgcc cggcgtagcg caacggcggc
     4201 atcagcgcca tcgtcagggc agccaccagc gcccctgccg ccggggccgc ccgcagccac
     4261 cccaacccac cggcccccac ctccagtata tcgcgcgcga agaccgggag cagcgcagtg
     4321 gccccaccca gcaacaccgc gaacatgtcg agcgttatcg ccgtaagaat cagtggcgtc
     4381 cgccagatga atcgcacgcc cgccagcagc gaggtgagat tgataggctc ggtgacccgg
     4441 tccacggggc gcggccggag caggcagaac agcagtgcca ccagcaccag cagtcccccg
     4501 gaggtagcgt acacgcccgc cgccccgccc cagagcgcga tgagcaagcc gcccagggca
     4561 gggcctacaa tggccgccgt ttgccacgcc ccactggacc atgctgcggc gctaccgtag
     4621 tgagtggaag gcaccacctg cgcgctcatc gcactgaagg cggggctttg gaacgagcgc
     4681 gccaccccga tgccgaatag cagcgcataa atggcaggca gcggccccgt gttgagggag
     4741 agtaccacca acccgaacgc gcacgcggca agcccgctca tcgcggcgac gactacccac
     4801 ttgcgctccc ggcggtcggc cacctgcccg gcaggcagcg cgagcagcaa cacgggcacg
     4861 atctccacca ggccgaccaa ccccagcgcc aggggatcgc gggtgcgctc gtacagctcc
     4921 cagcctaccg ccaccatcag catctgctcg gcgaacacgg caagaaagct gcccattatc
     4981 aggaggcgga agtcgcggaa ccgtagcgct gcaacggggt cgtggcgcgg gcgctcggcg
     5041 gttcctcccg gcggggcggg ggtctcaggc ggctctaccg ggaacatagg ttactattat
     5101 aagagacgaa accgccaaga cgcccagaac gccaagagcg cgccaagaag aaaatgatta
     5161 ttcaccgcag agacgcagag agcctgccct gagcgcagcc gaagggacgc agaggttacg
     5221 cagagtagaa atacaaggta ttctgagta
//
LOCUS       DASRWZ010000234        11073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1252144, whole genome shotgun sequence.
ACCESSION   DASRWZ010000234 DASRWZ010000000
VERSION     DASRWZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 11073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11073
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252144"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFH60_03695"
     CDS             complement(<1..346)
                     /locus_tag="VFH60_03695"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6312915.1"
                     /translation="MRLSDVSSLRGFSEQLGEGVTLTQLYPNAGDGSGLLALDLLEVE
                     AGGQTPVRASGEEQVLFVVSGTGELSQDGTTAGVKAGAVVSVGPFESYSIRNTDNVRL
                     RVLVSSSLLARTA"
     gene            823..963
                     /locus_tag="VFH60_03700"
     CDS             823..963
                     /locus_tag="VFH60_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312916.1"
                     /translation="MDTESAENQLPIIGIGCGALALFMVCVLTALAVGLFWVLQQGTA
                     VV"
     gene            1238..1870
                     /locus_tag="VFH60_03705"
     CDS             1238..1870
                     /locus_tag="VFH60_03705"
                     /inference="COORDINATES: protein motif:HMM:NF039508.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5701 family protein"
                     /protein_id="HET6312917.1"
                     /translation="MDLHQEFDRQFNNLLRKGYAEVANKSREEFSRYIRPLKDKLQDL
                     TVPEIDLERGHLPLVIVIKSDLVSTERAMSLAEKEGKEGTTKLYPHAPGDFQTIGNVT
                     VPDGMAYLLVDVDRGKENINLPPNEAIVLIEKAERSPLTIDEGVAILTQFPEFLVKNN
                     CYSLLASRYKGDQRVPAIWINANKNPNLGWCWDGNPHTWLGSASCKIRIG"
     gene            2246..5764
                     /locus_tag="VFH60_03710"
     CDS             2246..5764
                     /locus_tag="VFH60_03710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET6312918.1"
                     /translation="MTTAIRPQSSPSGPPLYTDEQYLQLVGRRNFGRIPSVMPMPNLI
                     QIQIDSFDWFKTQGLQELFEEISPITDFTGKNMELRFLDFHFGEPRYSVFECRQRDMT
                     YAAPLRVRVQLLIKETGEIKESEIFMGDFPLMTDTGTFVINGAERVVVSQLVRSPGVY
                     FTATEDANTGRQLFGAKLIPNRGAWLEFESSNKDVMSVKVDRKRKIPVTILLRAIGYS
                     DDDQLLELFKEVNTNPDHNYIRATMDKDGIHEREGKDAREEAVIEFYRRLRPGDPPTR
                     DNADSLLQSLLFNPRRYDLAKVGRYKLNKRLGIDVSLDTRVLTNEDLLKIVEHMVVLN
                     NRELDPLNHARPDDIDHLGNRRVRAVGELIQQQFRVGLLRMERVIKERMSIQDPESAS
                     PGALINIRPVVAAMREFFGGSQLSQFMDQTNPLSELNHKRRLSALGPGGLSRDRAGFD
                     VRDVHESHYGRICPVETPEGPNIGLIGSLATYARINEFGFIETPYRRVVNTVNNNADE
                     LVGHVLRENIEDAKGKVIAESGTPIDAALAKRIAATDLARVRVRPRLTEEIEYLSADV
                     EDNFVIAQANSPVDETNHFTARSVAARHHQQFLQEPPERVDYMDVSPKQVVSVATALI
                     PFLEHDDANRALMGANMQRQAVPLLMPRSPIVGTGIEWQAARDSGQVVLAKQDGVVTR
                     ADASLIVIRDDEGVEHEYNLAKFVRSNQDTCINQRPLIHSGDRLRAGHVIADSSSTET
                     GELALGQNILVAFMSFEGGNFEDAILLSQRLVRDDVFTSIHIEKYEVEARETKLGPEE
                     ITRDIPNVGEDSLKNLDEHGIIYIGAEVTANDILVGKITPKGETELTPEERLLRAIFG
                     EKAREVKDTSLRVPHGVRGKVIDVKQFSRANNDELPAGVSQMVRVSIAQKRKISVGDK
                     MAGRNGNKGVISRILPMEDMPFLPDGTPVDIILNPLGVPHRMNIGQILETHLGWAADK
                     LCFKVATPVFDGASEESIHDELERAGLPRDGKVDLYDGRTGDKFDNPVTGGQIYMLKL
                     AHLVEDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQEMLTV
                     KSDDVMGRVKTYEAIVKGESIMEVGVPESFKVLVKELQSLGLDVEVFTEDDVLVALSE
                     DAGADTMSELEGINLSGFEADERDRL"
     gene            5828..10078
                     /gene="rpoC"
                     /locus_tag="VFH60_03715"
                     /pseudo
     CDS             5828..10078
                     /gene="rpoC"
                     /locus_tag="VFH60_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            complement(10564..10881)
                     /locus_tag="VFH60_03720"
     CDS             complement(10564..10881)
                     /locus_tag="VFH60_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312919.1"
                     /translation="MFEVGKEMIAWLTLFVLLAVQIGGLTWWLRREFLPFVFNSKAPL
                     IYSTVLATDCVITWLVSMLDTPGGSSGAAMLAVLGVIQLVIVVVLALFFQWIVRSDLT
                     DIK"
BASE COUNT         2408 a         3532 c         3261 g         1872 t
ORIGIN      
        1 tggcagtacg cgccaacagc gacgaggaca ccagcacgcg cagccgtacg ttatcggtgt
       61 tccttataga atagctctcg aacgggccta cggataccac cgcgcccgcc ttcacccccg
      121 ccgtcgtgcc gtcctgcgac aactcgcccg tgcccgacac gacgaacagc acctgctcct
      181 ccccgctggc ccgcaccggc gtctgcccgc ccgcctccac ttccagcagg tcgagcgcga
      241 gcaagccgct gccatccccg gcgttcgggt aaagctgcgt cagcgtcacg ccctcgccca
      301 actgctccga aaaccctctc aagctagaca catcgctcag tcgcatgaat cacccactcc
      361 taacgggcca ctccgcccgc actcatctca aatcggcaat ccaaaatccg aaatctaaag
      421 tgcaaaaaac ccatccttca cttggaagga cgggcaagaa ttgtcccgcg gtaccacctt
      481 cgttggcgca cagcgcccac ttggtgaggg caccccacgc atgggtaggt ccctcggccc
      541 cggtaacgta gggcgacccc ggcgcagtct actgggcggc acgcgacgtg ctactgttca
      601 accggcggct caggaacgat tttcagcggg ctttcgggac cggtcgcacc aaccccggcc
      661 tctctgcgcc tacctgcacg cttactcttt ccgtcatagc ctgcaataca tataatgtga
      721 catttctagg atacaggaac aggaaagcag tgtcaagcgg acgctggtag tacaaacgtt
      781 ccgcctggag acgcaagaat cgttgaccag tgaggagttt ggatggatac cgagagtgca
      841 gaaaaccagt tacccattat cggcataggg tgcggcgcgc tggcgctgtt catggtgtgc
      901 gtgctgacgg cgctggccgt ggggctgttc tgggtgttgc agcagggcac cgctgtggtc
      961 taacccacgg tataaattag caggattgag attgttgaag tgaccccttt taccggcgca
     1021 aggccgcgca tgacgcctta cgggcgtgca gacaagtggt acttgggtgg tggagagcga
     1081 gattcttcgc ggagtttacc ctgagcccag ccgaagggct cagaatgaca aaggccgaag
     1141 tttggtccaa cacaatcagc actttgcaga taggccctga tcttccctct ttatctctag
     1201 ctatgattga acagcactaa cgtatgaggg cgtcctcatg gaccttcacc aagagtttga
     1261 cagacagttt aataacttgc ttcgaaaagg atacgcagag gttgctaaca aatctcgcga
     1321 agaattctca agatacataa gaccactgaa ggacaaacta caagatctta ccgttcctga
     1381 aatagatttg gaaagaggtc atttacctct cgtaattgta atcaagagtg atctcgtttc
     1441 cacagaaaga gctatgtcac tcgcagaaaa ggaaggaaag gaaggcacta ccaagttata
     1501 tccgcatgcg ccaggagact ttcaaaccat tgggaatgta actgttccgg atggcatggc
     1561 ttatctatta gttgatgtag atcgcggaaa ggagaatatt aatcttccac caaacgaggc
     1621 tatagtactg attgaaaagg cggagcgctc cccactaact atcgatgaag gagtagcaat
     1681 tcttacccag tttcctgaat ttcttgtaaa gaataactgc tactctttgc ttgcgtcgcg
     1741 ctacaagggt gaccaaagag ttcccgccat ctggataaat gccaataaaa accctaatct
     1801 cggttggtgt tgggatggaa acccacatac ctggttaggt tcagcttctt gcaaaattag
     1861 gattggctga agcttacggc ctatagggag ccgaacaacg gccgaattgt tcatagtaag
     1921 cacaacggcc ggaaaacgac ccgctctgtg tcggttatag tgccaggttg agggtataga
     1981 gcagaatgcg gctgatggga caccaaaatc ccacttgaca actccctacc ccccggttat
     2041 aatgacctgt ccagggtaaa ttccccattt atgacagacc tacaaacgca aaagtaaccc
     2101 ccacctgtcc cacggctgcc cacaatgtcg tgagccgacg ggaagggtgt tttgcgtctt
     2161 ttggctgtcc actctcgcgg cccctggcga agctgagcgt tcaagctggt tcagcgcgtt
     2221 cttgagttat aggagcaatg tatttatgac gacagctata cgcccgcaga gcagtccctc
     2281 aggtcccccc ctctacaccg acgaacagta tctccaactg gtaggtaggc gcaacttcgg
     2341 caggatccct tccgtcatgc cgatgcccaa cctcatccag attcagatag attcgttcga
     2401 ttggttcaag acgcagggcc tccaggagct gttcgaggaa atctccccga ttaccgactt
     2461 taccggcaag aacatggagc tgcgcttcct cgacttccat tttggcgagc cacgttactc
     2521 cgtatttgaa tgccgccagc gcgatatgac ctacgccgct cccctgcgcg tcagggtgca
     2581 gctactcatc aaagaaacag gcgaaatcaa agagtctgaa atattcatgg gcgacttccc
     2641 gctcatgacc gataccggca cgttcgtcat caacggtgcc gagcgcgtcg tcgtgtccca
     2701 gttggtgcgc tcccccggcg tctatttcac cgccaccgaa gacgccaaca ccggccgcca
     2761 gcttttcggg gccaagctca tccccaaccg gggtgcctgg ctcgagttcg agtcctccaa
     2821 caaggacgtg atgtccgtca aggtggaccg caagcgcaag atccccgtga cgatcctgct
     2881 gcgcgctatc ggctactccg acgacgacca gctactggag ttgttcaagg aagtcaacac
     2941 caaccccgac cacaactaca tccgggccac gatggacaag gacggcatcc acgagcgcga
     3001 gggtaaggac gctcgcgagg aggccgtgat cgagttctac cgcaggctcc gcccaggcga
     3061 cccgcccacc cgcgacaacg ccgacagcct cctgcaatcg ctcctgttca acccccgccg
     3121 ctatgacctg gcgaaggtgg gccgctacaa gctcaacaag cgactcggca tcgacgtgtc
     3181 cctcgacacc cgcgtgctga ccaacgagga cctgctcaag atagtggagc acatggtagt
     3241 gctcaacaac cgagagctag accccctcaa ccacgcccgc cccgacgaca tcgaccacct
     3301 gggcaaccgc cgcgtgcgcg cggtgggcga gctaatccag cagcagttcc gagtcggcct
     3361 cctgcgcatg gagcgcgtca tcaaggagcg catgtcgatc caggaccctg agagcgcatc
     3421 ccccggcgcg ctgataaaca tccgcccggt ggtcgccgcc atgcgcgagt tcttcggcgg
     3481 ctcccagctt tcgcagttca tggaccagac caacccgctc tccgagctga accacaagcg
     3541 ccgcctgtcg gccctcggcc ccggcggcct ctcccgcgac cgcgcgggct tcgacgtgcg
     3601 cgacgtgcac gagagccact acggccgcat ctgcccggtg gagaccccgg aaggccccaa
     3661 catcggcctt atcggctccc tggcgaccta cgcccgcatc aacgagttcg gcttcatcga
     3721 gaccccctac cgcagggtgg tcaacacggt gaacaacaac gccgacgagc ttgtgggcca
     3781 cgtgctgcgc gagaacatcg aggacgccaa gggcaaggtc atcgctgaga gcggcacgcc
     3841 tatcgacgcg gccctcgcca agcgcatcgc cgctaccgac ctggcccgcg tccgcgtgcg
     3901 cccccgcctc accgaagaaa tagagtacct gtcggccgac gtggaggaca acttcgtaat
     3961 cgcccaggct aactccccgg tggacgagac caatcacttc accgctcgct cggtggccgc
     4021 ccgccaccac cagcagttcc tccaggaacc gcctgagcgc gtggactaca tggacgtgtc
     4081 gcccaagcag gtggtgtcgg tggctacggc cctgataccc ttcctcgagc acgacgacgc
     4141 caaccgcgct ctcatgggcg cgaacatgca gcgccaggcc gtgcccctcc tcatgcctcg
     4201 ctcgcctatc gtgggcaccg gcatcgagtg gcaggccgcc cgcgactccg gccaggtggt
     4261 gctcgccaag caggacggtg tggtcacccg cgccgatgcc tccctcatcg tcatacgcga
     4321 cgacgagggt gtggagcacg agtacaacct ggcgaagttc gtgcgctcca accaggacac
     4381 ctgcatcaac cagaggccgc tcatccattc gggcgaccgc ctccgggcag gccacgtaat
     4441 cgcagactcc tcgtccaccg agacgggcga gcttgcgctg ggccagaaca tcctcgtagc
     4501 cttcatgagc ttcgagggcg gcaacttcga ggacgctatc ctgctgtcgc agcgcctggt
     4561 gcgcgacgac gtctttacct ccatacatat agagaagtac gaggtagagg cccgcgagac
     4621 caagctaggc cccgaagaaa tcacccgcga tatacccaac gttggcgagg actctctgaa
     4681 gaacctcgac gagcatggca tcatttatat aggtgccgaa gtcaccgcga acgacatcct
     4741 tgtgggtaaa attaccccca agggcgagac ggagctaacc ccggaggagc gactgttgag
     4801 ggctatcttc ggtgagaagg cccgcgaggt gaaagacacc tccctgcgcg tgccccatgg
     4861 cgtgcgcggc aaggttatcg acgtaaagca gttctcccgc gccaacaacg acgagctacc
     4921 ggcgggcgtg agccagatgg tgcgcgtatc tattgcccag aagcgcaaaa tctcggtggg
     4981 cgacaagatg gcggggcgga acggcaacaa gggcgttatc tcccgcatcc tcccgatgga
     5041 ggacatgccc ttcctgcccg acggcacccc ggtggacatc atcctgaacc ccctgggcgt
     5101 gccccaccgt atgaacatcg gccagatact tgagacccac ctgggttggg ccgccgacaa
     5161 gctctgcttc aaggtcgcca ccccggtgtt cgacggcgcg agcgaggaga gcatccacga
     5221 cgagctggag cgggcgggcc tgccacgcga cggcaaggtg gacctctacg acggccgcac
     5281 cggcgacaag ttcgacaacc ccgtgacagg gggacagatt tacatgctca agctggccca
     5341 cctcgtggag gacaagatcc acgcccggtc cacaggcccc tacagcctcg ttacgcagca
     5401 gccgctgggt ggcaaggcgc agttcggcgg ccagcggttc ggtgagatgg aagtgtgggc
     5461 gctcgaagct tacggcgcgg cgcacaccct gcaagagatg ctcaccgtca agtccgacga
     5521 cgttatgggc cgcgtaaaga cctatgaggc catcgtcaag ggcgagagca tcatggaagt
     5581 cggcgtgccc gagtcgttca aggtgctcgt caaggagcta cagtcgctcg gcctcgacgt
     5641 ggaagtattc accgaggacg acgtgttggt agccctcagc gaggacgccg gggccgacac
     5701 catgtccgaa ctggaaggca tcaacctgag cggcttcgag gccgacgagc gggacaggct
     5761 ttaggtaatt acgaattaca gcagaactca tgtaattcgt aattcgtaat tctccaaagg
     5821 agatacgatg ttagaggtga acaatttcag tgcaatacgg attagcctgg cctcgccgga
     5881 gaagatcaag gactggtcgc acggtgaggt gaccaagccg gagacgatca actaccgcac
     5941 gctcaagccg gagcgcgacg ggctgttctg cgagcgtatc ttcggtcccc agaaggactg
     6001 ggaatgctac tgcggcaagt acaagagggt acgatataag ggcaccatct gcgacaagtg
     6061 cggcgtggaa gtgacccgct cccgcgtgcg ccgcgagcgc atggggcaca tcgagcttgc
     6121 ctcccccgtg tcgcacatct ggtacgtcaa gggcacacct tctcgcctcg gcttgctgct
     6181 cgacatctcg ccgcgcaact tggagcgggt gctctacttc gctacctata tagtgaccaa
     6241 cgttgacgag gaagagcgcc agcggcagct cgagcgcgta cagaccgagg gcgagcgccg
     6301 catcgaggaa gtcaccgagc gcacagcttc gcgcatcagc gagttgcagg aaggcttgca
     6361 ggacgagatc gccgagatcc aggccagcga ccgcgatgcc cgcaccgagc ttgacgccgg
     6421 gctgaaggct tccaagaagg agctagactc ccagcgacgt gccatcgagc gccagctcaa
     6481 gaagcagatg ggcaatgagg ccgtcgagga catctacctg gcaggggcca cccgccccgt
     6541 catccaggcg ggccagacta tcaccgacgg cacgcttgag cgcctgaagg acatagccga
     6601 ggagtacctg ttcgacgtgc aggaggacct gcgccgccag tctcagcact cgcaggacgt
     6661 gacgggcgct acccgcgacc agcgttccta cgtggccttg caggagagcg agcagatcca
     6721 ggtccgcgcc aaccgcgagg ttgaggacct gcgccgacag atggaagagc ggttggacca
     6781 gctagagtct atccaggtct accagttgct gtcggagcag tcctaccgcg aaatgtcaga
     6841 ggccttcccc ggcgtgttcc gcgcgggcat gggcgccgag gcggtgctgg agatcgtgga
     6901 caagctgaac ctggacaaca tggcgaacga gatgcgccac gaaatccaga cggtgggcgg
     6961 ccagcgccgc aagaaggcca ccaagcgcct gcgcgtgatc gaggccttcc gcaagtcggg
     7021 caaccacccc tcctggatga tgttcaagct gctgcccgtg ataccccctg agcttcgccc
     7081 gatggtgcag ctcgacggcg gccgcttcgc cacgtccgac ctgaacgacc tgtaccggcg
     7141 cgtaatcaac cgcaacaacc gcctcaagag gctgttggag cttggcgcgc ctgagatcat
     7201 catccgcaac gagaagcgca tgcttcaaga agcggtggat gccctcatcg acaacggccg
     7261 ccgtggccgc gtggtgtccg gctccggcaa gcaccgcctg aagtccctca gcgacatgct
     7321 caagggcaag cagggccgct tccgccagaa cctgctcggc aagcgcgtgg actactcggg
     7381 ccgctcggtc atcgtggtag gcccggagct taagctgcac cagtgcggcc tgcccaagaa
     7441 gatggcgctc gagctgttca agccgttcgt tatgcgccgc ctcgtggccc agggccacgc
     7501 ccataacatc aagagcgcca agcgcatagt cgagcgcgtg cgccccgaag tgtgggacgt
     7561 gctcgaagag gtcatcaagg actacctagt gctgctcaac cgcgccccgt ccctgcaccg
     7621 cctctccatc caggcgttcg aggcagtgct catcgaaggc tcggctatcc agctccaccc
     7681 gatggtctgc tcggccttca atgccgactt cgacggcgac cagatggcgg tacacgtgcc
     7741 gctctcctcc gccgcccagc gcgaggcgag ggagcgcatg ctctcgatct acaacatgct
     7801 ctcgcccgcc aacggcgacc ccatcgtgtc cccttcgcag gacatcgtgc tcggctgcta
     7861 ctacatgaca gtcatgcgcc ccggcaccga gggtgaaggc aagatatttg cctcgcccga
     7921 agaggccgag cttgcgtaca acagcaacat catcaacctg caagcgccca tcatcgtgcg
     7981 catgcccggc ctgaacggca agtccgagct tgtggagacc acggtcgggc gcgtcatgtt
     8041 caacaccgtg gtggcccgct tcaacgagga gctttcgccc gaagagcgca tcccttatcg
     8101 caaccaggcg atggacaagg gcgcgctgcg cgggctcatg gccgaggtgc acctcaagat
     8161 gggctcgcgc cgcaccgccg aggtagcgga cgtcttcaag aggctgggct tccagttcgc
     8221 caccaagtcg gggatgtccg tggccgtggc ggatatcgag ataccgcgca ccaagccgga
     8281 ccgcctcacc aaggccgacg ccgacgtgga cacggtagag aagagctacc gccgaggtat
     8341 catcaccgag caggagcgct acgaggaggt catccgcatc tggaacgaga ccaccgacga
     8401 gcttaccgcc gagcttgaga aggtgatgga cccctacggc ctcatctaca cgctggcgaa
     8461 ctcgggcgcg accaaggcca agttcaagca gatacgccag ttggccgcca tgcgcggtct
     8521 catggccgac ccgtcgggcc gcatcatcga gatgcctatc cgctcgaact tccgcgaagg
     8581 tctcaccgtg ctggaatact tcgtatcgac ccacggcggc cgcaagggcc tcgccgacac
     8641 cgcactccgc accgcagacg ccgggtacct cacccgccgc ctcgtggacg tggcgcagga
     8701 cgtgatcgtg accaccgagg actgcggaag cacctccggc gtgtggatcg cctggccggg
     8761 ccgcgacgtt gccgacaacc tggcacagcg cgtaatcggc cgttacgccg cacaccccgt
     8821 gactcacgcc ataaccggcg aaatccttgt tgacgccaac gaggagctaa ccgaggacgc
     8881 gctcaatgag ttcatgcgga gcgtcgagga gtcggtccaa ccgctacgcg agaagctggc
     8941 gaaggaagga cggcgcggcg acgagctgga caagatcacc ggcgctgaga aggccaagtt
     9001 cggcatcatg acccgctccg cgctcatgtg cgacgtgagc cacggcgtct gccagaagtg
     9061 ctacgggcgc gctctgcaca acagcagggt ggtggacctg ggtgaagcgg tgggtatcat
     9121 cgcggcccag tccatcggcg agccgggcac gcagcttacc ctgcgcacct tccacacggg
     9181 cggcgtggcc tctaccgaag acatcacgca gggtctaccc cgcgtggagg agcttttcga
     9241 ggcgcgcgtg cccaagggcc aggctatcct cgctgaaatc gacggtgtgg tgaacatcgt
     9301 ccgcgaggag accggcgaga ccagaatccg cctgacccac agcgaaatct tcgacgacga
     9361 gtaccccctg ccccgccacc acgagctgct ggtggacgag ggcgccgagg tgctggaggg
     9421 catgccgctc ttccgctcca acgccaaggg cgaagaggac gacgtgaccg tggcccgcac
     9481 ctccggcatc gtggtgctgg agaagggcaa gaagcggctc atcgtgcgcg gcgaggaccg
     9541 cgagctacgg gagtactcgg taccccacag cgcgcagttc gaagtccgcg agggcgaccg
     9601 cgtatcggcg ggacagcccc tcacgcgtgg cgcgcagaac ccacaggaaa tcctgcacat
     9661 tctcggtcgc gaggccgtgc agcgctacct ggtggacgaa gtgcagcgcg tgtaccgctc
     9721 gcagggtgtg aacgtaaacg acaagcatat cgagatcatc gtgcgccaga tgctgcgcaa
     9781 ggtgcgtatc gatagccccg gcgacaccga gatgctgccc ggcgagatgg tggagaggtt
     9841 caacttcgcc aacatcaacg ctcgcgcaat cgcggcaggc ggcgaggcgg ctacggctac
     9901 ccccgtgctg ctcggcatca ccaaggcgtc gctctcgacc gactcggtcc tctcggccgc
     9961 ctccttccag gagacgacca gggtgctcac cgaagccgcg ataaacggcc aggtggaccg
    10021 cctgcgcggc ctcaaggaga acgtgatcat cggcaagctg atcccggccg ggtcgggcgc
    10081 tcgctacggt gcggacggcg gcgacctgcg catcatatcg accacaggta tcaccggcct
    10141 cggctctcct aacatcgatg actacctatc ccactccgag ggcgcgggcg gcttcgaagg
    10201 cgagtacccc ggcgggggtg tggacgacta cctgaacaac ctacccggct tcgcgccgga
    10261 cggcgatggg atgtcctcgg aagaggccac ccgcgccctg ctcgaaccgg aagcctacct
    10321 ggagtcgatc gagcctaccg aggaagaagc cggcgaggat gaggtcagcg acgtgcccct
    10381 ctccgacgac ggcccgaccg aggacgcagc cgaagacgac acgacaggcg acgatggcga
    10441 taataccgac gccgaggaca acctcggcta ggtccgccgc gctgtagaga gcacgaaaga
    10501 ggaggggctc ttagcggaga ccctcctctt ttagcgtcag gaatggatag cgctcgtcgg
    10561 gtgctacttt atgtcagtga ggtcgctcct gactatccac tggaagaaga gcgcgagcac
    10621 gaccacaatg accaactgga tcacgccaag cacggcaagc atggccgcac cggacgagcc
    10681 gccgggtgtg tccaacatcg acacgagcca ggtgattacg cagtcggtgg ccagaacggt
    10741 gctatagatt agcggggcct tgctgttgaa cacgaaaggc aagaactcgc ggcgcaacca
    10801 ccaggtgagg ccgcctatct ggacggcaag cagcacaaac aacgtaagcc aggcaatcat
    10861 ctctttacca acctcgaaca agtagtttca attcataata catctgattg caagggtaag
    10921 gttcaaggga agacatttca atacataatt gccctctcaa gaacaaagtg cctaacataa
    10981 gcacacaaga tccgcctttg tcattctgag cgtagcgcag aatctcgtcc tccgccggga
    11041 tgttcccctg ttccgcagga gactctgtgc ggt
//
LOCUS       DASRWZ010000235         4814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1321015, whole genome shotgun sequence.
ACCESSION   DASRWZ010000235 DASRWZ010000000
VERSION     DASRWZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321015"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..765)
                     /locus_tag="VFH60_03725"
     CDS             complement(103..765)
                     /locus_tag="VFH60_03725"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6312920.1"
                     /translation="MSRIVDLSHRLAPGMPSYPGMLEPQFRTWFTHEESASRGTYSPG
                     TTFQIATYELGSNTGTYVDAPFHRHPGAPDLAGLPLEKLADLPGVVGEGAEEGEVPWQ
                     AFEGVDLRGKAVLVRTGWSRRWGEPGYFRSGPYLGEEVCRKLVEAGATLVGIDCANID
                     NMSDPARPAHTILLAAGIPIVEHLRDLDQLPQNGFRFFAVPPAIEQGTSFTVRAFAIV
                     ES"
     gene            complement(863..1840)
                     /gene="meaB"
                     /locus_tag="VFH60_03730"
     CDS             complement(863..1840)
                     /gene="meaB"
                     /locus_tag="VFH60_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011013089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET6312921.1"
                     /translation="MTATQNSEPKTRNTVPALVDRTLSGDRAAASRLMTIVENRRTGY
                     RETMRLLMPHTGRAQILGVTGPPGAGKSTLVSELIRAYREQGKTVGVVAVDPTSAVSG
                     GALLGDRIRMLAYYQDPGVFVRSMASRGQLGGLAAATVDVARVLDAMGRDIVIIETVG
                     VGQDEVSIAGLADTSLLVEVPGMGDDVQAIKAGVMEIADVFVINKADREGADRLANQI
                     KTVMRLLPHPEDAWVPPIVKTVAAEGKGIPELLEAIAKHQGYLAAEGRLEKDRRARLQ
                     TEVMERARDLALRRILARVEQSGDLSRVLDDLQARKIDPATAARMLVDE"
     gene            complement(1995..2465)
                     /locus_tag="VFH60_03735"
     CDS             complement(1995..2465)
                     /locus_tag="VFH60_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET6312922.1"
                     /translation="MVAKSPSELDEQEASSEAGRKIRVLIAKPGLDGHDRGAKVIARA
                     LRDAGMEVIYTGLHQTPEMIAEAALQEDVDAVGLSILSGAHMALFPRIMEELRTRGLD
                     DVLVYAGGIIPDEDIPAIKEMGVAGVFGPGTPMQDAVRFVKENVTPRHSLEEEE"
     gene            complement(2788..3813)
                     /locus_tag="VFH60_03740"
     CDS             complement(2788..3813)
                     /locus_tag="VFH60_03740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6312923.1"
                     /translation="MENVSYRQMVDSDHGQVSPLWMETWGYTDYEFLKRRIGADPNYM
                     EHTFLASAPDGEVLATAHYWLFDLRDVDGRPRRAGCVSNVVTKEKARKQGHARRLMEM
                     ALERMPHDGCEWTLLFTSEMGRALYLRLGYRAYSLPYARGVLSGSPLTLAGNYTITRS
                     DLTPQPRYWQAIAPIYEAYNRARPLTVARDAAYWQGLHVRKLERPPRGRTVHLFLAHS
                     TREGREVGYAIAYLSNHEAEPPAGSEQGFVLSEIAILPGHEEAIPHLLSAFAAAARGE
                     RVAGTSFLPGDAGVDKALRVLLEQPMEELNDRSIMARPLEGGLSQAEVEAIMAAPNVA
                     FWPLDEF"
     gene            4127..>4814
                     /locus_tag="VFH60_03745"
     CDS             4127..>4814
                     /locus_tag="VFH60_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SipW-dependent-type signal peptide-containing
                     protein"
                     /protein_id="HET6312924.1"
                     /translation="MVNLGTLRNTSNLKKVLLTVMVLGMATGVLGSGTFATFTAQTTN
                     PNNTFVAGTLVLSNTKTGGTTCFSTGGGSTDSNSNSACESLFSANVRKPGESASQTLT
                     VKNEGSLAASALKLFSTACGNANSTGESYFGTGLPCGKVQLYIQQYSDSSFTTPSACL
                     YGGATGATCDFSDATKTLSAFQGAYGSSGGGLTVGSGLAAGASVYVKIAVQLPTDANN
                     SFQGRQATMDF"
BASE COUNT          847 a         1581 c         1470 g          916 t
ORIGIN      
        1 cagcttcttt agattcttcg ctacgctcag aatgacatgg gccactctgg tcctccagtt
       61 gttaagttga tactgcttgc ggctcgtggc caagccagat gactacgact ctacaatggc
      121 gaaggcgcgc accgtgaacg aggtgccctg ctctatcgcg ggcgggacgg cgaagaaccg
      181 gaagccattt tgcggcagtt ggtccaggtc gcgcaggtgc tcgacgatcg gtatgcccgc
      241 cgccagcaga atggtgtggg cgggtcgcgc ggggtcggac atgttgtcga tgttggcgca
      301 gtcgatgccc accagggtcg ccccggcctc caccagcttg cggcacactt cctcgcccag
      361 gtacggcccg gagcggaagt agccaggctc tccccagcgc ctcgaccagc cggtacgcac
      421 cagcacggcc ttgccccgca ggtccacccc ctcgaacgcc tgccagggca cctcgccttc
      481 ctccgcccct tcccctacca cgcccggcag gtcggccagc ttctccagcg gcaggccggc
      541 aaggtcgggc gcgccggggt ggcgatggaa aggcgcgtcc acgtaagtgc cggtgttgct
      601 ccccagctcg taagtggcta tctggaaggt cgtaccgggc gagtaggtgc cccgcgacgc
      661 gctctcctca tgcgtaaacc acgtacgaaa ctgcggctcc agcatccccg gatacgaagg
      721 catccccggc gcaagcctat gactgaggtc tacaatacgc gacaaaccag cactccttcg
      781 gagagtaacg agtgatgagg actgaggact gagaggacgc ttactcagtc ctcagtcctc
      841 agtcctcatc actcgttact cgtcactcgt ccaccagcat tcttgcggcg gtggcggggt
      901 ctattttgcg ggcctggagg tcgtccagga cgcgggagag gtcgccgctt tgctctacgc
      961 gggccaggat gcggcggagg gcgaggtcgc gggcgcgctc catcacctcg gtttggaggc
     1021 gggcgcgccg gtctttttcg aggcggccct cggctgccag gtagccctgg tgtttggcta
     1081 tggcttccag caactcaggg atgcccttgc cctcggcggc caccgtcttg acgatgggcg
     1141 gcacccaggc gtcttcgggg tgggggagga ggcgcatgac cgtcttgatc tggttcgcca
     1201 ggcggtccgc gccttctcgg tcggccttgt tgatgacgaa cacgtccgcg atctccatca
     1261 cgcccgcctt gatggcctgc acgtcgtcgc ccatgcccgg cacctcgacc agcaggctcg
     1321 tgtcggccaa ccccgcgatg gatacctcgt cctggcccac gcccaccgtc tcgatgatga
     1381 caatatcgcg gcccatagcg tccagcacgc gcgccacgtc cacggtcgct gctgccagcc
     1441 cgccgagctg cccccgcgag gccatagagc gcacgaacac gccggggtcc tggtaatagg
     1501 cgagcatgcg gatgcggtcg cccaggagcg cgcccccgct caccgccgag gtcgggtcca
     1561 cggctaccac gcccaccgtc ttaccctgct cgcggtacgc cctgataagc tcgctcacca
     1621 acgtagactt gcccgcgccc ggcggcccgg tgacgcccag tatttgcgcg cggccggtgt
     1681 gcggcatgag caggcgcatc gtctcgcggt agcctgtccg gcggttctcc acgatagtca
     1741 tcaggcgcga agccgccgcc cggtcgccgg agagggtacg gtccaccagg gcggggaccg
     1801 tgttacgagt cttgggttct gagttttggg tggctgtcat ataagtaggt gagtgacaag
     1861 tgacgagtga tgaggctgag tggcactcac ccaaacaatc cttaaacttt gcgcgttctt
     1921 tgcgcccttt gcgtctttgc gttttcgtct ctttactcag tcctcagtcc tcgtcactcg
     1981 tcactcgtca ctcgtcactc ctcctcttcc agggagtgcc ggggtgtgac gttctccttc
     2041 acgaagcgga cggcatcttg catgggggtg ccggggccga acacacccgc gacgcccatc
     2101 tccttgatag cgggtatgtc ctcgtcgggg atgatgcccc cggcgtacac cagcacgtca
     2161 tcgaggccgc gagtgcgcag ttcttccatg atacgaggga agagggccat gtgcgcgccg
     2221 gagaggatgg agaggcccac cgcgtccacg tcttcctgca gggcggcctc ggcaatcatt
     2281 tcaggggtct ggtgcaggcc ggtataaatg acctccatgc ccgcgtctcg caaagccctg
     2341 gcgatcacct tggccccgcg gtcgtgcccg tcgaggcccg gcttggcgat gagcacgcgt
     2401 atcttgcggc ccgcctcgct gctcgcttcc tgctcgtcca gttcagatgg cgactttgcc
     2461 accattttgc gcctcctaag tgtcgggggt gttcgtgtgt gcttgctcca tcttacaact
     2521 taagccacgc gcagtaaagg aatggtgcgt ggtccacgga ggttaagcgt agcggggacg
     2581 ccctgtgggg tttgccctgc caaagaaatg gcgggctggc aaggggcaaa gtcccctacg
     2641 gggactgagg aacatcaggt agtctccgta ggagacttcg tctctggtaa gcccgccaat
     2701 tcattggcag ggcggggtgg gcagggcaca cctacagggc gtcccctacg gcacgtgcct
     2761 gccgcaagcg atcgcaccct gccccactca aaactcgtcc aacggccaga acgcgacgtt
     2821 gggcgcggcc atgatggcct ccacttccgc ctggctcaaa cctccctcta agggacgcgc
     2881 catgatggac ctgtcgttaa gctcctccat tggctgctcg agcaataccc tcaaagcctt
     2941 gtctaccccg gcatcaccgg gcaaaaagga ggtgccggcc acacgctctc ccctagccgc
     3001 agccgcgaac gccgacagca ggtgcggtat agcctcctcg tggccgggga gtatggctat
     3061 ctcggacagc acgaagccct gttccgaccc ggcgggaggc tcggcttcgt ggttagagag
     3121 atatgctatc gcataaccca cttcgcggcc ttcccgcgtg ctatgggcta agaaaaggtg
     3181 aactgtacgg ccccttggtg gcctctcaag cttgcgcacg tgcagaccct gccagtaagc
     3241 ggcgtcgcgt gccaccgtaa gtggccgggc gcggttgtat gcctcgtata tgggcgctat
     3301 agcttgccag tagcgcggct gcggcgtcag gtccgagcgc gtgatggtat agttgccagc
     3361 gagggttagt ggcgacccgg aaagcacccc gcgtgcgtac ggcagcgaat aggcgcggta
     3421 gcccaggcgc aggtagagcg cgcggcccat ctccgaggtg aagagcaggg tccactcgca
     3481 gccgtcgtgg ggcatgcgtt cgagcgccat ctccatgagc ctgcgggcgt ggccctgctt
     3541 gcgggccttc tctttcgtga caacgttgga cacgcaaccc gccctgcgcg gcctgccgtc
     3601 cacgtctcgc aggtcgaaaa gccagtaatg cgcggtcgcg aggacttcgc cgtcaggggc
     3661 ggaggccaaa aaggtgtgct ccatgtagtt ggggtcagcc ccaatgcgcc tcttcaggaa
     3721 ctcgtagtcc gtgtaccccc acgtctccat ccagagggga cttacctggc cgtggtcgga
     3781 gtcgaccatc tgcctgtagc ttacgttttc cacaaaccgc ctcctttgtc cgcgtgcaag
     3841 ttgccgggcc tcccacacgt cccactttat agcggcagag gtccctaact cgtcgggact
     3901 tccggcccta agtcctaggg aagatagtac tgaatgatac aggaatatga gaccatgctc
     3961 ctattaccct ttagtcccgc atcgggtaaa gtgctaatat cgatagcaga ctgtggcacc
     4021 cgacaactat gctacaggcc cggttttata catccggcct tcttcaacgt gcttactgaa
     4081 gcataggggc agaccggaac aacattatct ctggaggaat tatccaatgg ttaatctagg
     4141 tactctgagg aacaccagca acctgaagaa agtgctgctc accgtaatgg tgctcggcat
     4201 ggcgacgggg gtcttaggct ccggcacgtt cgctactttc acagcccaga cgaccaaccc
     4261 gaacaacacg ttcgtggcgg gcaccctcgt gctgagcaac accaagacgg gcggcaccac
     4321 gtgcttctcg acaggcggcg gcagcaccga cagcaacagc aacagcgcct gcgaatcgct
     4381 gttcagcgcc aacgtgcgca agcccggcga atcggcctcg cagacgctca ccgtgaagaa
     4441 cgaaggctcc ctggccgcct cggccctcaa gctcttctcc acagcttgcg gcaacgccaa
     4501 cagcacgggc gagtcgtact tcggcaccgg cctgccctgc ggcaaggtcc agctttacat
     4561 ccagcagtac tccgactcca gtttcactac cccctcagcc tgcctctacg gtggggccac
     4621 gggcgctact tgcgacttct cggacgccac caagacgctc tccgccttcc agggcgcgta
     4681 cggctcttcg ggcggcgggc tgacggtcgg cagcgggttg gccgctgggg cttcggtcta
     4741 cgtcaagata gccgtgcaac tccccacgga cgccaacaac agcttccagg gccgccaggc
     4801 caccatggac ttca
//
LOCUS       DASRWZ010000236         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_704141, whole genome shotgun sequence.
ACCESSION   DASRWZ010000236 DASRWZ010000000
VERSION     DASRWZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704141"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..>1669)
                     /locus_tag="VFH60_03750"
     CDS             complement(164..>1669)
                     /locus_tag="VFH60_03750"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET6312925.1"
                     /translation="VYGVVVLALAAAPILPAALGWEEKRLRWAGLLLLALLAGAGRTL
                     LEHPPLTSGDLAYYNGTETSAPVRIVAHVSGEPVYRDRSQRLRLTARSLALPGADEPV
                     QVSGDMLAVLPRYPGFYAGEQVTLYGTLTPPPQIEGFDYAGYLARQGVYSYMLYPGVL
                     DRKATTEGGLLQLISGVRGAVRQALREGLPEPQAALVVGVVTGDRTSLPEDLQQAFSI
                     SGTSHILAISGQNISLLVGFVFLAYAGGKERRKMPPWLLGTVLVLLAAYTVFTGATPA
                     VVRAALMSGVLLASQAVGRRFDPISALAVSAALMSLVDPDLLLDVGFLLSFAAMLGLV
                     QVSPLVLQGLSKARLPGLLGVPLSASIGAQLTTAPLVLLLTGRFSTVSPLATLTTEFM
                     LLPLMIAGIAGGFLGALFGQAGSLLSLLAWPPATWMIESVRWWASLPAASLEVGQVGP
                     GWIVGYYLLAALLVWWAREGHVRFKLQPGELALAACAVAAWTVLLGLLVGG"
BASE COUNT          331 a          532 c          577 g          229 t
ORIGIN      
        1 cgagacgagc cggcaaagag agttatggac aggattatac atgatgccgc aaggcgcagg
       61 aagggacaac cgggttatag tggcggcccc tttagtcctg tgtggcggtg gaggttggta
      121 ttaggaatgc ccgggagaga agatgcccgc taaattgtgc ctgctagccg cccaccagca
      181 gccccagcaa gacagtccag gccgcaaccg cacaggctgc cagtgccagt tcgccgggtt
      241 ggagcttgaa ccggacgtgg ccttcgcgcg cccaccagac gagcagcgcg gcgaggaggt
      301 agtagcccac tatccagcca ggcccaacct gaccgacttc gagtgaggcc gcgggcaggc
      361 tcgcccacca gcggaccgac tcgatcatcc aggtggcggg gggccaggcg aggagcgaca
      421 gcagggagcc ggcctgcccg aatagcgcgc ccaggaaccc accggcaatg cctgcgatca
      481 taagtggtag gagcatgaat tcggtcgtga gggtggcgag cggcgaaacc gtggagaagc
      541 ggcccgtgag cagcaggacc aggggcgcgg tggtcagttg cgcgccgatg ctggcggaaa
      601 gtggtacgcc cagcagccct ggtaggcgcg ctttgctcag cccctgaagc accaggggcg
      661 agacctgcac cagccccagc atcgcggcga aactcagcag gaagcccacg tctaacagca
      721 gatcggggtc aaccagcgac atgagcgcgg ccgacacggc cagggccgat attgggtcga
      781 agcgccgccc taccgcctgc gatgccagca gcacgccgct cataagggcg gccctcacca
      841 cggcgggggt agccccggtg aagacagtat acgcggccag caagacgagt acggtgccga
      901 gcagccaggg gggcatcttg cgccgctcct tgccgcccgc gtaggccagg aagacgaagc
      961 cgaccagcaa ggagatgttt tgcccggaga tcgccaggat gtggctcgtg ccgcttatgc
     1021 tgaacgcctg ctgcaagtct tcgggaaggc tcgtgcggtc tcccgttact acccccacca
     1081 cgagcgccgc ctggggttcg ggcagcccct cgcgtagcgc ctgccgcact gccccgcgca
     1141 ctcccgaaat tagctgcaac agcccgcctt cagtggtggc cttgcggtcg agtacgccgg
     1201 ggtaaagcat gtaagagtag accccctgcc gcgccaggta gccggcgtag tcgaatcctt
     1261 cgatttgcgg cggcggggtc agggtgccgt acagggtcac ttgctcgccc gcatagaagc
     1321 cagggtagcg cggcagaaca gccagcatat cccctgagac ttgcacaggc tcgtccgcgc
     1381 cgggcagcgc gagcgaacgc gcggtcagcc gcaggcgttg cgagcggtcc cggtagacgg
     1441 gttcgccact gacgtgggct acgatgcgaa caggggccga ggtctccgtc ccgttgtagt
     1501 aagcgaggtc gccggaggtg agcggggggt gctcgagcag ggtgcgcccg gcacccgcca
     1561 acagcgcgag aagcaacagt cccgcccagc gcagccgctt ttcttcccaa cccagagcag
     1621 cgggtaagat cggtgcggcc gcaagggcga gcaccacgac accgtacac
//
LOCUS       DASRWZ010000237         1893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1046759, whole genome shotgun sequence.
ACCESSION   DASRWZ010000237 DASRWZ010000000
VERSION     DASRWZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046759"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1819
                     /locus_tag="VFH60_03755"
     CDS             <1..1819
                     /locus_tag="VFH60_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6312926.1"
                     /translation="LNRSVAIKVLEPALAVDPRAVERFKREAVTAANLEHPAIVRVYD
                     VQQEGHLHYIAMRYVQGTTLRDILRDNGPLPFDAILGITRTVAEALHYAHRHGVIHRD
                     VKPGNILVEPDGTVLLTDFGIARAADSSQAALTATGSVMGTADYLAPEQISGRPADAR
                     SDIYSLGVVLYEMLTGVTPFAGENTATVLYRQVYDSPPPIRSINPRLPAELQPVVDRV
                     LAKNPTSRYADPLDMAKELEEVGQWLPPGSPWLNRGRGGQTVAYPNGGREPSQPYNMQ
                     PPQGVARGTSPQPQRVVPRTYSESDQHRDTTPPARYEAQAHVPPRRVGRRRVMLFVAL
                     LLVAAGVSVLAVAANIISIARPVDPVQPRFQPPGPATYTGPLREGNGENISIPRALQP
                     PALDGDLSDWAGASPGPFAAPYNVLTARGVTPQGPDDLSAQFQFAWDDTNFYVAAVIT
                     DDVHVQFAGTRGYDLFKGDDIEMWFDLDLAGDFDKPHDFVKGTNSGDDFQLGLSPGDF
                     GELLPEAVFWDPQRNADRNKLVSVAVQKRLTNNGYTLEAAIPWLAFGNFRPQPGMAIG
                     FVASAGDNDQAGKAIQEIMVSTSPNMQWRKPATFGNLFF"
BASE COUNT          363 a          577 c          640 g          313 t
ORIGIN      
        1 gctgaacagg tcggtggcta taaaggtgct tgagcctgcg ctggcggtgg accccagggc
       61 ggtggagagg ttcaagcggg aagcggtaac ggcggcgaat cttgagcacc cggccatcgt
      121 tagggtgtac gacgttcagc aagaggggca cctgcactac atagcgatgc gctacgtgca
      181 gggtacgacg ctgcgcgaca tcttgcgcga caacggcccc ctgccctttg acgccatcct
      241 gggcatcacg cgcacagtgg ccgaggcgct gcattacgcg cataggcacg gggtcatcca
      301 ccgcgacgtg aagccaggca acatactggt cgagccggac ggcacggtgc tgctcaccga
      361 cttcggcata gcgcgtgcgg cggatagctc acaggcggcg ctaacggcta cagggtcggt
      421 gatgggcacg gcggactacc tggcaccgga gcagataagt ggccgcccgg cggacgctcg
      481 ctccgacatt tactcgctgg gcgtggtgct ctacgagatg ctgacgggcg tgacgccgtt
      541 cgcgggggag aacacggcta cggtactcta ccggcaggtg tatgatagcc cgccgcccat
      601 acgctcgata aacccgcgcc tcccagccga attacagccg gtggtggacc gcgtgctcgc
      661 caagaacccg acctcgcgct acgccgaccc gttggacatg gcgaaagagc tggaagaggt
      721 agggcagtgg ctgccgcccg gctcgccctg gctcaacagg ggcaggggag ggcagacggt
      781 agcgtatccg aacgggggca gagagccgag ccagccttac aacatgcaac ctccgcaggg
      841 cgtggcgcgc ggcaccagcc cacagccaca gcgcgtggtg ccgcgcactt actcggagag
      901 cgaccagcac cgcgatacga cacctccggc gaggtacgag gcgcaagcac acgtgccgcc
      961 tcgacgcgtg ggccgcaggc gggtgatgct cttcgtggcg ctcctgctgg tggctgccgg
     1021 ggtgtcggtg ctggcggtgg cggcgaacat catctctatc gcccgcccgg tcgatcccgt
     1081 ccagccgagg ttccagccgc ccggacccgc cacatatacg gggccgttgc gcgagggcaa
     1141 cggggagaat ataagcatcc cacgcgccct gcaacctccg gcgctggacg gcgatctatc
     1201 ggactgggcg ggcgcgtcgc ctgggccgtt cgccgcgccc tacaacgtgc tcaccgccag
     1261 gggtgtgacg ccgcaggggc cggacgacct gagcgcgcag ttccagttcg cgtgggacga
     1321 taccaacttc tacgttgcgg cggtcatcac ggacgacgta cacgtgcaat tcgctgggac
     1381 gcggggctac gatttattca aaggcgacga tatagagatg tggttcgatt tggacctggc
     1441 gggggatttc gacaagccgc acgactttgt gaagggtacg aacagcgggg acgattttca
     1501 gttgggcttg tcgccgggcg acttcggcga gttgctgcct gaagcggtgt tttgggaccc
     1561 gcagcgaaat gcggaccgca ataaactggt gagcgtggcg gtacagaagc gcctaacgaa
     1621 caacggctac accctggaag cggcgatacc ctggcttgcg ttcggcaact tccgccccca
     1681 acctggcatg gctataggtt tcgtggcgtc ggcgggggat aacgaccaag ccggcaaggc
     1741 tatccaggag attatggtct ccaccagccc caatatgcag tggcgcaagc ctgccacgtt
     1801 tggcaatctc ttcttctaag gggagaatta aacaaacagc aaaaagcctc ggtcaggcgc
     1861 tccgcgccga actgaggctt tttgttgtct tgc
//
LOCUS       DASRWZ010000238         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1321087, whole genome shotgun sequence.
ACCESSION   DASRWZ010000238 DASRWZ010000000
VERSION     DASRWZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321087"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFH60_03760"
     CDS             <1..216
                     /locus_tag="VFH60_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HET6312927.1"
                     /translation="LADKLHNARSILADFGASGEGAWSRFKRGRLQQEWYYRWLAEAL
                     CAPREGEVPLPFCAEFRQVVEELFGAT"
     gene            complement(433..2382)
                     /locus_tag="VFH60_03765"
     CDS             complement(433..2382)
                     /locus_tag="VFH60_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6312928.1"
                     /translation="MPRFRFTLLAKFSITSLALLAVMGVLLGMGLTRHFEEQAIEQQK
                     MAAASLVPPVVGPSLSEELLANGAHGEEYEDIQRALSFLGGSNLVAARVWNQDGVVIY
                     SDLPYLVGRSQPLTPQVREGLEGRISAEIRPIASKEAAAVRGYGDIMTVYTPLQMPGS
                     NRIGAVFEGDYDVTDLRQRISYTNGFLWFSIAGGFLFLYVSLFTIVRNASSRLERQSA
                     ENAHLYGEAQHQLAERQVAEERIKRQVENLQALRDIDIAITSNPDLALTLNVILTQVR
                     TQLKVDAADVLLLDERAKELEYAAGSGFRAEAVAGTRLWLGQGYAGKAALQRRTIGVD
                     RMTNTGDLTRARLLEGEDFVAYCAVPLVAKDKVRGVLEVFQRGPLPDDPEWLKFLEAL
                     AGQAAIAVDNAALFDSLQRSNAELARAYDKTLEGWSRALDLRDKETEGHSRRVTEMTV
                     HLAREMGVADEELVHIHRGALLHDIGKVGIPDSILHKPGPLSDEEWATMRMHPVYAYE
                     LLRPIAFLEPALDIPYCHHEKWDGTGYPLGLKGEEIPLAARIFAIADVWDALRSNRPY
                     RSARPREMALAYIMEQSGKHFDPDVVGAFLRMDWVNVPHQQSILAGGDLPVTATVAGN
                     TVTDQGKGAEVVGDPVPAVAGARPG"
BASE COUNT          443 a          819 c          846 g          473 t
ORIGIN      
        1 ctggccgaca agctacacaa cgcccgtagc atcctggccg atttcggagc tagcggcgag
       61 ggcgcgtggt cgcgcttcaa gaggggccgc cttcagcagg agtggtacta ccgatggctg
      121 gcggaagcgt tgtgcgcgcc gcgggagggc gaggtgccgc tgcccttctg cgcggagttc
      181 cggcaggtgg tggaggagtt gtttggtgcc acgtaggtgc atgacgctct cgtgacgccc
      241 tggatgccct gtggctaaag ccacgggctt acaggaggca aagtcccctt cggggactga
      301 ggcacatcat gtagtctccg aaggagactt cgctacttcc cagcccgtgg ctttagccac
      361 agggcgtcat gcagggcgtc cccgcagggc acccatgcca tgctacacac cacccatcgt
      421 acctgccaag acctaacccg gccgggcacc cgcgaccgca ggcacagggt ccccgaccac
      481 ttctgctccc ttgccctggt cggtaaccgt gttacccgcg acggtggcgg tgacgggcaa
      541 atctcccccg gcgaggatgc tttgctggtg gggcacgttg acccagtcca tgcgcaggaa
      601 cgcccccacc acgtcggggt cgaagtgctt gcccgactgc tccatgatgt aggccagcgc
      661 catctcgcgg ggccgcgccg agcggtaggg gcggttcgag cgcagggcgt cccacacgtc
      721 ggctatggcg aagatgcggg ccgcgagcgg tatctcctct ccctttagac cgagcgggta
      781 gccggtgccg tcccatttct cgtggtggca gtagggaata tcgagcgcag gctccaggaa
      841 ggcgatgggg cgtagcagtt cgtaggcgta taccgggtgc atacgcatgg tcgcccattc
      901 ctcgtcgctg agagggccgg gcttgtgcaa aatcgagtcg gggatgccca ccttgccgat
      961 atcgtgcagg agcgcgccgc gatggatgtg caccagctcc tcatcggcca cgcccatctc
     1021 tcgcgccagg tgcacggtca tctcggtgac gcggcggctg tggccctccg tctccttgtc
     1081 gcgcaggtcg agagcgcggg accagccttc cagcgtcttg tcgtacgccc tggcaagctc
     1141 cgcgttggag cgttgcaggc tatcgaacag ggctgcgttg tccacggcaa tcgcggcctg
     1201 ccccgccagc gcctccagga acttgagcca ctcggggtcg tcgggcagcg gccctcgctg
     1261 gaacacctcg agcaccccgc gcaccttgtc cttggcgacc agcggtaccg cgcagtaggc
     1321 cacgaagtcc tcgccctcca gcaaccgggc gcgggtgagg tcgcccgtat tggtcatcct
     1381 atcgacgccg atagtgcgcc gttgcagggc cgccttgccc gcgtagccct gtcctagcca
     1441 gaggcgagtg cccgctaccg cttcagcccg gaagccgctg cccgccgcgt actcaagttc
     1501 cttggcccgc tcgtcgagca gcagcacgtc ggcagcgtca accttcaact gggtgcgcac
     1561 ctgggtgaga ataacgttaa gggtcagggc caggtcgggg ttggaggtaa tagcgatgtc
     1621 gatgtcgcgc agggcctgca aattctccac ctgccgcttg atgcgctcct cggctacctg
     1681 gcgctcggct agctggtgct gggcctcgcc gtagaggtgg gcgttctccg cgctctggcg
     1741 ttcgaggcgg ctcgaggcgt tgcgcacaat cgtgaatagc gacacgtaga ggaaaaggaa
     1801 cccgcccgcg atgctgaacc aaaggaagcc gttggtgtag ctgatgcgct gccgcaggtc
     1861 ggtaacgtcg taatcgccct cgaacacggc ccctatgcgg ttgctgcccg gcatttgcag
     1921 gggagtatag accgtcataa tgtcgccata cccgcgtacg gcggcggcct ctttggaggc
     1981 aatgggacgg atttcggcgg agatgcgccc ttccagcccc tccctgacct ggggggtaag
     2041 cggctggctc ctgcccacaa ggtagggcag gtcggagtaa atgaccaccc catcctggtt
     2101 ccacaccctg gccgcgacca ggttggagcc gcccaggaag gagagcgcgc gctggatgtc
     2161 ctcgtactcc tcgccatgcg cgccattcgc cagaagctcc tcgctgagag aggggccgac
     2221 cacggggggc acgagagagg cggcggccat cttttgctgc tcgattgcct gttcctcgaa
     2281 atgtcgtgtc aggcccatgc cgagcagcac ccccatgaca gccaggaggg ccaggctcgt
     2341 tatcgagaac ttcgcgagta aagtgaaccg gaaccggggc atacagccat accttagtgt
     2401 agtgcgtctc ccggcacgcg gccgcgaggg tgcttgtgcg cctctaagct attatagatg
     2461 cgtgttctgc cgcttgtaac tcagacatat ggataatatc tcataggaca ttagtacttt
     2521 ggcgtataat aagcccgccg ctcgcgagtg ccgagtgccg agtgccgaga gaagaaatga
     2581 g
//
LOCUS       DASRWZ010000239         4076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_498686, whole genome shotgun sequence.
ACCESSION   DASRWZ010000239 DASRWZ010000000
VERSION     DASRWZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498686"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..866
                     /locus_tag="VFH60_03770"
     CDS             153..866
                     /locus_tag="VFH60_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6312929.1"
                     /translation="MRNVFYVAGKELRSYFVSPVAYIIVAFWLVITGLFFTASATAGD
                     ASMRNVFGVIPILLLLISPALTMRLLAEESRTGTLELLLTAPVKDWSVVVGKFLGALG
                     LFVAMMFLTVLYPLILVLFRGDPDWGPIASGYLGLLLLGMAFLSVGLLASSLTSNQIL
                     SAVIALAILLVLFIITVLTSNVPPNVAAVLAKLSIGDRYDPFTRGIVDLTDVVYFLTF
                     TGAVIFITIQIVEARRYRA"
     gene            863..2491
                     /locus_tag="VFH60_03775"
     CDS             863..2491
                     /locus_tag="VFH60_03775"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HET6312930.1"
                     /translation="MNKSARALVATLAGFAALGFAGIALFYWAAFQSLTLDFGSMDLT
                     LRILIIAAIVSFAIYVLAAPESVGKAAGKRSTRLTTNALVASIAAVAVAVVINFISEN
                     APTVRADWTAGGDFSLSPQTQSVLDSLNNRPENVQAVAFIRTENQGGRQQADDLLREY
                     SSRQRKLTYQFIDPFRQPAAAVGYGITREGVVVFTDGKKREIADSITERDFTSAIVRL
                     GQNKVNTIAFLTGHGERDMNSFEAGGYSTARESLEQNNYQTVAWSLVTSPTLTVQDVT
                     VLVIVEPKQALTAQEVQTIQSYLDAGGHLLVMMDPAMSPEAQKPLHDLLTPYGLEPVQ
                     GVALDPAIQLDPSDAGILGPDTYPSNDITSDLERNKLRTAYLASMGFRDNGAKSGFVI
                     RPLVRTVSSAPTSWLETTLTGTTAPAFDAENDLPGPVILAALIEPADPVTGAVTSTTT
                     PNTRIVAFGDGDFASNNILQQQLPLYNSDLFGNTVSYLAGANELVSIRPKDPSTPRTV
                     ALDAGQRHLVLVSTVLGLPLLVSLAGVMVWWRRK"
     gene            2493..3323
                     /locus_tag="VFH60_03780"
     CDS             2493..3323
                     /locus_tag="VFH60_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET6312931.1"
                     /translation="MERYRTTIILLVLLVALGGLAMVLGGNRGATDTPGVPTPAPVTY
                     VWQDDNPVTGLDAVSGTQKLVIIKDVTTTIWSIREPISDTADPFAVGNLADQLQNLQA
                     TSTITDASDLAQFGLADPGVTLTVAFSDTAGTKRTLLLGGKTIDGSAYYAKTSDKADV
                     YLVSNSIVEPLRSWFDAPPKVVPTATPLLPTIGPTQEVTGTGTITGTVLPAGTTTPAG
                     ATTPGAGTTTPQAQATGVITGTETMTAQQPAAANATTPETTPLLATATPAGAGATPSP
                     "
     gene            complement(3395..>4076)
                     /locus_tag="VFH60_03785"
     CDS             complement(3395..>4076)
                     /locus_tag="VFH60_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET6312932.1"
                     /translation="ANVLFATLDPTTRLIKLPEGQEVLLTDTVGFIQRLPTQLVAAFR
                     ATLEEIQDADVLLHVVALTHPNAEEQAETVDRVLEELGITEKPKVVALNKIDRLFPEL
                     VGSARANGADGAADAPSGLDVANISARISEEMGLGSNYVPVSARLNWGLTELLERIQQ
                     TLSDTLVGVRVLIPYNESELVSRFHERGIINSEEHTGTGTLIEGRLPKRLVRAYERYE
                     ERNGHDPK"
BASE COUNT          750 a         1297 c         1264 g          765 t
ORIGIN      
        1 gcggacgagt cgttcgagga ccgcgagtac gacgactacc atggcgatag ggacggcgac
       61 gaggccggtg accactcagg agcggacgag gacgatgcgg aagcggtggc agcgcccagg
      121 acgcgggtag tggcccgcag gagggtgcga ggatgagaaa cgtattctac gtcgcgggca
      181 aggaattgcg ctcctatttt gtgtcaccag ttgcgtatat catcgtggcc ttctggctgg
      241 ttataaccgg gctgttcttt acggccagtg ccacggcggg cgatgccagc atgcgcaacg
      301 tgttcggcgt catccccatc ctgctgttgc tgatctcccc ggccctcacg atgaggctgc
      361 tggcggagga atcgcgcacg ggcacgctcg aactgctgct gaccgcgccc gtcaaggact
      421 ggtcggtggt ggtgggcaag ttcctgggag cgctcggcct ctttgtggcg atgatgttcc
      481 tcacggtgct gtacccgttg atactcgtcc tcttccgggg cgaccccgac tgggggccga
      541 ttgcgagcgg ctacctcggc ctgctattgc ttgggatggc gttcctgtcg gtgggcctgc
      601 tggcttcttc gctcacgtcc aaccagattc tctcggcggt gatcgcgctc gcgatattgc
      661 tggtgctgtt tatcatcacc gtcctgacca gcaacgtccc gccaaatgtg gcggcggtgc
      721 tcgccaagct gtcgataggc gaccgctacg acccgttcac acgcggcatc gtggacctga
      781 cggacgtggt ctacttcctg acgttcacgg gggccgtcat ctttatcacc attcaaattg
      841 ttgaagccag gaggtacagg gcatgaacaa atccgcacgt gccctcgtcg ccacgcttgc
      901 cggtttcgcg gccctgggct tcgcgggcat cgctcttttc tactgggctg ccttccagag
      961 cctcacgcta gacttcggca gcatggacct gacgttgcgc atcctgataa tcgccgccat
     1021 cgtcagcttt gcgatttatg tgctggcagc gcccgaatcg gtgggcaagg ctgccggtaa
     1081 gcgcagcacg cgccttacca ctaacgcatt ggtggcgtct attgctgcgg tggccgtcgc
     1141 ggtcgtgatc aacttcataa gcgagaacgc gcccacggtg cgggccgact ggacggcagg
     1201 tggcgacttc tcgctgtcac cccagaccca gagcgtgctt gacagcctga acaacaggcc
     1261 cgagaacgtg caggcagtgg ccttcatccg gaccgaaaac cagggtggtc ggcagcaagc
     1321 ggacgacctg cttcgcgagt acagcagccg ccagcgcaag cttacctacc agttcatcga
     1381 cccgttccgg cagcctgccg ccgctgtggg ctacggcata acgcgggaag gtgtggtcgt
     1441 cttcaccgac gggaagaagc gcgagattgc cgacagcatc accgagcgcg acttcaccag
     1501 cgctatcgta cggctcggcc agaacaaggt aaacaccatc gcgttcctca ccggacacgg
     1561 cgagcgcgac atgaacagct tcgaggcggg gggctattca acggcgcggg agtcgttgga
     1621 gcaaaacaac taccagacgg ttgcctggag cctagttacc tcaccgacgc tcacggtgca
     1681 agacgtgacg gtgctggtga tcgtggagcc gaaacaggcg ctgacggccc aggaggtaca
     1741 gaccatccag agctaccttg acgccggcgg gcacttgctt gtgatgatgg accccgcgat
     1801 gtcgccggaa gcgcagaagc cgctgcacga cctcctcacg ccgtacggcc tcgaacccgt
     1861 gcagggtgtg gccctcgatc ctgctatcca actagacccc agcgatgcgg gtatcctcgg
     1921 accagatacg tacccttcaa acgacatcac gagcgacctt gaacgcaaca agctacgtac
     1981 ggcctacctt gcttcgatgg ggttccggga caacggcgca aaatcgggct tcgtcatcag
     2041 gccgctagtg cgcaccgtta gctcggctcc taccagctgg ctggagacta cccttaccgg
     2101 cacgacggca ccggctttcg acgctgagaa cgacctgccc ggccctgtga tcctggccgc
     2161 tctgatcgag cctgccgacc cggtgacagg cgctgtcacc agcacgacta cgcctaacac
     2221 gcgtatcgtg gcgttcgggg acggcgactt cgcgagcaac aacatcttgc agcagcaact
     2281 gccgctgtac aacagcgacc ttttcggcaa cacggtgtca tacctcgcgg gcgcgaacga
     2341 gctagtgtcg atacggccca aagacccgtc tacgccccgc acggtggcac tggacgccgg
     2401 gcagcgccac ctggtactgg ttagcaccgt actgggccta cctctgttgg tgtccctggc
     2461 cggtgtgatg gtgtggtgga ggcgcaagta acatggaacg ctatcgcact actatcattc
     2521 tgctggtcct gctggtcgcc ctgggcgggc tggcgatggt cctgggcggg aaccgcgggg
     2581 cgaccgacac ccccggcgtg ccgacccccg ccccggtgac ttacgtgtgg caggacgaca
     2641 acccggtgac tggcctcgac gcggtgagtg gcacgcagaa gcttgtgatt atcaaggacg
     2701 tgaccaccac catctggtcg atacgagaac ccatctcgga taccgccgac ccgttcgcgg
     2761 tcggcaatct cgcggaccaa ttgcagaact tgcaagccac aagtacgatt acagatgctt
     2821 ccgacctggc acaattcggg ctggcggacc ccggcgtcac gctgacggtc gcgttcagcg
     2881 acacggcggg gaccaagcgc acgttgctgt tgggcggcaa gaccatagac ggctcggctt
     2941 actacgcaaa gacctccgat aaggcggatg tctatctcgt gagcaattcc atcgtggagc
     3001 cattgcgctc ttggttcgac gctccgccga aggtcgttcc gacggcgacg cccttgctgc
     3061 cgacgattgg tcctacgcag gaggtcacgg gcacgggcac aatcaccggc acggtgctgc
     3121 ctgccggcac gaccaccccg gcgggtgcga ctacaccggg cgcgggcacg accacacccc
     3181 aggcgcaggc gacgggcgtt atcacaggca cggagacgat gaccgcgcag caaccggctg
     3241 ctgcgaacgc caccactcct gagactaccc cgctgctggc gactgctacc ccggcgggtg
     3301 ctggcgcgac accttcaccg tagagcatcg aatcgacaat taagagaagg ggacggctca
     3361 aaaccgtccc cttcttcgtt ggaatcgcta acccctactt tgggtcgtgg ccgttgcgct
     3421 cttcgtagcg ttcgtaggcg cggaccaatc tcttaggaag gcgtccctct atgagagtgc
     3481 cggtgccggt gtgctcctcc gagttgatga tgcccctctc gtggaaacgc gataccagct
     3541 cgctctcgtt gtaggggatc agcacgcgca cccccaccag ggtatcgctc aatgtctgct
     3601 gtattcgctc cagaagctct gtaaggcccc agttgaggcg tgcggatacc ggtacatagt
     3661 tcgaccccag ccccatctcc tcagagatgc gtgcgctgat gttggccacg tccaacccac
     3721 tcggggcatc ggcggccccg tccgcgccat tcgccctggc gctgccgaca agctcgggga
     3781 agagcctgtc tattttgttc aaggcaacta ccttgggctt ctccgttatg cccaactctt
     3841 cgagcacgcg gtccacagtc tcggcctgct cctcggcgtt ggggtgggta agggccacaa
     3901 cgtggaggag cacgtccgca tcctggatct cttcgagcgt ggcgcggaag gcggcgacaa
     3961 gctgggtcgg caagcgctgg atgaagccta ccgtgtcggt aagcaacacc tcctgcccct
     4021 ccggcagctt gatgaggcgt gtggtcgggt ccagggtcgc gaacagcacg ttcgcg
//
LOCUS       DASRWZ010000240         4453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_88018,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000240 DASRWZ010000000
VERSION     DASRWZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88018"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1888)
                     /locus_tag="VFH60_03790"
     CDS             complement(<1..1888)
                     /locus_tag="VFH60_03790"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET6312933.1"
                     /translation="MESPARNVQAGLKPPADRQNSNGGRSSMNNSPQAHILNRVNALE
                     TTVDELQQAMRRAGLLPEPAPTFDVSQFATPTQESLIGPATQVETPEETAQANSVPPI
                     TVPPANAIERCQACGAANAPNAPLCTWCGEPLVTSLGLPPVEGPAKAANQPARPQGGE
                     VRAVPAKTTRSYEVERVSVGAPPPPLPRQTTTQVSGPAPKREPFKLQFDVLGKAEFWL
                     NKVGIVLFLFGVVFLFRYAIDNGWLDEQARVGAGLLLGSMLLGFGVLLHAQRRHLSQV
                     LLGGAIATYYITGYAAHNIFPMLNVSHESALAYMAVVTALAFLLSLWHGEAVLALIGV
                     VGGLLTPFALGTSEVQVPGLITYTALILLGTSAIYMYRGWRAMLGCAFLGGAANLFMG
                     HALAWATWGDQWWQQGAATLGDRAFLQAGIIVVLLAFWVVPVVRQVLCRKDPARWPVP
                     TLDKAKYPELSKLAGLDVHVLSAMVPLLSLVGSWFLWSDFWTVGPPLVSWETWGVVSL
                     LGAAVMLGAFLLLHRVERSLAYVHALLATTLLTLAIVQLLDADAIFLAFAVEALGLHL
                     IARRFSDKGMELWGHALFGVVGLWIAPRLAASLGGMSPFYNSRTLLDLVVLGLGLAAS
                     FAFAR"
     gene            2057..4285
                     /locus_tag="VFH60_03795"
     CDS             2057..4285
                     /locus_tag="VFH60_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6312934.1"
                     /translation="MASLNNLPHRLTSFVGREREKDEAIGLLARCRLLTLTGTGGSGK
                     TRLALEVASCVAEDYPQGAWLVELSSITDPALPVQEVATLFGVREEPGTPLLDTLAAY
                     LHSTRTLLVLDNCEHLVDECAELADRLLTACPGLRVLATSRQPLGLDYEVAHRVPPMS
                     MPDATQLPQVEELERYEALRLFVERATAIRSDFVVGQEHVSALVELAAKLDGLPLAIE
                     LAAARTRVLSVQQIVERLDERFRLLSTTSRSASPRHRSLQAVMDWSYELLSEEERAVL
                     RSLSMFAGAFTLEAVQAVSGLEVDEFETIDLLSRLVDRSLLNVDERGGAATYRMLETI
                     RRYGIERLEEAGEATPARDRLLEYYTDLAESTENELLSVSQPLWLERLEREHDNLRAS
                     LEWGWTSERVRNRQLGLRLAGALVWFWYFRGYLSEGRTWLERLLGEEGARRPSVGLAK
                     SLSAAGVLSYLQSDYLVARERLEEGLRVWRALGDLRGTAFALTFLGRVAQQLDDPRAA
                     ELGAESVALFREHGDKWGLALSLDFLGEVARERGDHARAEELHRESMSLYLEVGHSWG
                     VALELSHFGHVALRAGDYATARQRLEEAIEIQRTVGDKWMLAWTLHNLGHVVRAQGDH
                     AQAAVFHDESLQLFRELGGLGGMPAAAASRDALFEAPSLVPQPPSPAPQDNPHNLTPR
                     EIEVLQLIAEGLTDAQVADRLVLSTRTVQAHLRSIYSKLDITTRSAATRYALEHGLVG
                     GR"
BASE COUNT          831 a         1424 c         1446 g          752 t
ORIGIN      
        1 gcctcgcgaa cgcaaacgag gccgccagcc caagccccag caccacgagg tccagaaggg
       61 tgcgggaatt gtagaagggc gacataccgc ccaagctagc tgccaggcgt ggcgcaatcc
      121 acagccccac aacgccgaag agcgcatggc cccacaactc catgcctttg tccgagaacc
      181 gtcgggcgat gagatgcagc cccagcgctt ccaccgcaaa ggccaggaag atagcgtcgg
      241 cgtcaagtag ctgcacaatc gcaagcgtca gcagcgtggt ggcaagcagc gcatgcacgt
      301 aggcaaggct ccgttccacg cggtgcagca gcaggaacgc gccaagcatc accgccgcgc
      361 caagcaggct gaccacgccc cacgtctccc acgacaccag tggaggcccc acagtccaga
      421 agtcggacca taggaaccag gaccccacga gggagagcag aggcaccata gccgacagca
      481 catggacgtc aaggccggca agcttggaaa gctccgggta tttggcctta tccagcgttg
      541 gcaccggcca ccgggccggg tccttgcgac atagtacctg gcgcacaaca ggcaccaccc
      601 agaaggcgag caggaccact atgatgcccg cttgcaggaa agcccggtcg cccagcgtag
      661 ccgcgccttg ctgccaccac tggtcgcccc acgtggccca ggcgagcgcg tggcccatga
      721 agagattggc tgcccctcca aggaacgcac agccgagcat ggcccgccaa ccccggtaca
      781 tgtagatagc gctggtgcct aatagtatca aagcggtgta cgtgatgagg ccgggcactt
      841 gcacctcgct ggtgccgagg gcgaaaggag tcagcagccc tcccaccacg ccgattagcg
      901 cgagcaccgc ctctccgtgc catagggaga gcaagaaggc cagcgcagtg acaaccgcca
      961 tgtaggcgag cgcggactcg tgagatacgt tcaacatcgg aaatatgttg tgggcggcgt
     1021 agccggtgat gtagtaggtg gcaatcgcgc cgccgaggag cacctgggag aggtgccgcc
     1081 gctgggcgtg cagtagcaca ccgaagccca gcagcatgga acctagcagc agcccagccc
     1141 ctactctcgc ctgttcgtcg agccagccgt tatcaatcgc gtacctgaac aggaacacca
     1201 ccccgaacag gaagagcacg atgcccacct tgttcagcca gaactccgct ttcccaagta
     1261 cgtcgaattg cagcttgaac ggctcgcgct tgggtgcagg cccacttacc tgggtcgtgg
     1321 tttggcgcgg gaggggaggc gggggcgctc ctaccgaaac acgttcaacc tcgtacgacc
     1381 tcgtcgtctt cgcgggcacc gcccgtacct caccaccttg tggcctggcg ggttggttcg
     1441 ccgctttagc aggaccttca accggaggca gccccagcga agtgaccagg ggttcgccac
     1501 accacgtgca gagtggtgcg ttgggggcat tcgccgcacc gcaagcctgg cagcgttcta
     1561 tcgcattggc cgggggcacg gtaatgggcg ggacgctatt ggcctgggcg gtctcctccg
     1621 gggtctccac ctgtgtggct ggtcctataa ggctctcctg ggtgggtgtt gcgaactgcg
     1681 acacgtcgaa cgtgggtgca ggctccggca acaggcccgc ccggcgcatg gcctgttgca
     1741 gttcatcgac tgtcgtttcg agagcattta cgcggttgag tatgtgagcc tggggcgagt
     1801 tgttcatcga gcttcgacct ccattgctgt tctgcctgtc tgccgggggc ttgagacctg
     1861 cctgcacgtt gcgcgcaggc gactccacct caataacacc ttacccgttc gacccagtga
     1921 gggctacggc aaatgcacct agctttgtta ccgcccctat actgccgaat aggtaatttt
     1981 gcctagatgt cgccggcaaa gggcatgtgg cctgggcctt gcggctgtat aattgaggta
     2041 tcgaaaggag acatgcttgg cctcgctaaa caacctgcct catcgactca cgagcttcgt
     2101 cggccgcgag cgcgaaaagg acgaagcaat agggctgctg gcacgctgcc gcctgctgac
     2161 cctcacgggc acgggcggta gcggcaagac tcgtcttgcg ctggaagtgg cctcgtgcgt
     2221 ggcggaagac tacccacagg gtgcctggct ggtcgagctt tcctctatta cggaccccgc
     2281 gctccccgtg caggaggtcg cgaccctgtt cggcgtgcgc gaggaacccg gcacgcccct
     2341 gctcgatacg ctggccgcct acctccactc cacccgtacg ctcctggtgc tggacaattg
     2401 cgagcacctc gtggacgagt gcgcggagct ggccgaccgc ctgctgacgg cgtgccccgg
     2461 cctgcgagtc ctcgcgacca gccgccagcc cctcggcctc gactacgagg tggcccaccg
     2521 cgtgccccct atgtcgatgc cggacgccac gcagcttccc caggtcgagg aactggagcg
     2581 gtatgaagcc ctgcgcctct tcgtggaaag ggccacggct atccgctcgg actttgtcgt
     2641 gggacaggag cacgtcagcg cgcttgtgga gcttgccgcc aaactcgacg gcctccccct
     2701 ggcgatagag ctcgcggcgg cccgcacgag ggtgctttcc gtacagcaga tcgtagagcg
     2761 ccttgacgag cgtttcaggc tgctgtccac caccagccgg agcgcctcgc cccgccaccg
     2821 ctcgttgcag gctgtgatgg actggagcta cgagctgctt tccgaggagg agcgagccgt
     2881 gctcaggtcg ctgtcgatgt tcgcgggggc cttcaccctg gaggccgtgc aggcggtgtc
     2941 gggcctggaa gtggacgaat tcgagacgat cgatttgcta tcgcggttgg tggacaggtc
     3001 gctcctgaac gtagacgagc gcggcggtgc ggccacgtac cgcatgcttg aaacgattcg
     3061 gcgttatgga atcgagcgcc ttgaggaggc cggggaagcc acccccgcac gcgaccgcct
     3121 cctggaatac tatacggacc tggcggagag caccgagaac gaactgctca gcgtcagcca
     3181 gcccctctgg ctggagcgcc tggagcggga gcacgacaat ctgcgggcct ccctcgaatg
     3241 gggctggact tcggagcgtg taaggaaccg ccagctagga ttgaggctgg ccggggcgct
     3301 ggtctggttc tggtacttcc gcggctatct tagcgaaggc cggacctggc tggaaaggct
     3361 gcttggcgag gaaggcgcca ggcggccgag tgtgggtctc gccaagtcgc tgagcgcggc
     3421 aggggttctc tcgtacctcc agagcgacta cctggttgcc agggagcggc tggaagaggg
     3481 gctacgggtg tggcgggcgc tgggcgatct gaggggtacc gctttcgctc tcacgttcct
     3541 ggggcgggtg gcccagcaac tggacgaccc tcgcgcggcg gaactgggcg ctgagagcgt
     3601 ggcgctcttc cgcgaacacg gcgacaagtg ggggctggcc ctctcgctcg actttctggg
     3661 tgaggtggcc cgcgaacgag gcgaccacgc acgcgccgag gagctgcacc gtgagagtat
     3721 gtccctgtac cttgaggtgg gccatagctg gggcgtggcc ctcgaactga gccattttgg
     3781 gcacgtcgcc ctgcgcgccg gtgactatgc caccgctcgc cagcggctgg aagaagcaat
     3841 cgagatacag cgaacggtgg gcgacaagtg gatgctggcc tggacgctgc acaacctcgg
     3901 ccacgtggtg agggctcagg gcgaccacgc tcaggcggca gtcttccacg acgagagcct
     3961 gcaactgttc cgagagcttg gtggtcttgg tggcatgccc gccgccgctg catcgcgaga
     4021 cgcactgttc gaagcgccct ccctggtacc tcaacctccg agtcccgcgc cacaggacaa
     4081 cccgcacaac ctgaccccgc gcgaaatcga ggtgttgcag cttatcgctg aaggtctcac
     4141 cgacgcgcag gttgcagacc ggctggtcct gagtacgcgc acggtgcagg cccacctgcg
     4201 ctccatctac agcaaactgg acatcactac ccgtagcgcc gcgacgcgct acgcgctgga
     4261 gcatggcctg gtcggaggac gctagacgag actcgccaac gccgggccgt acccaacaga
     4321 ccataggtga cgcgttttgg ccgctaacgt gccggtcctg gcaacctggg cagaaaattc
     4381 cttcagaatt ggtctctgta cccccttgac aaagcggcag ttcgggtgta agatagtctc
     4441 acttcggagg tgt
//
LOCUS       DASRWZ010000241         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_498955, whole genome shotgun sequence.
ACCESSION   DASRWZ010000241 DASRWZ010000000
VERSION     DASRWZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498955"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="VFH60_03800"
     CDS             <1..1397
                     /locus_tag="VFH60_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312935.1"
                     /translation="VKVYAEMLAGSYAAIKQANPEATVLVGGLSIHDRTNAGMAFLEQ
                     VVAASGGKLNFDVLSIHPYMPDRHPESTDPRTVVQNFPYRLEMSYKWLQDHGAPNKEI
                     WVTETGYSTCNPCGNLGVSEEEQARRLVRLYALAMAAPNVTHFTYFQIKDKFNHPPGD
                     MFGNMAIMRNDMSQKPAYIAYRVMAHTLEGATFTGQGPLMRAVQNRWQPQFDRYHYRF
                     TRSGKNVSVIWKLGQPETVTMPLTQPSVEVVSSRGEKLDVQVHNGAITLRISEDPIYV
                     VESNPTTSAALDPATDAASPTGFRASLRFQPYWQNNGGLPLFGFPISGERNETNPIDG
                     KQYIVQWFERARFEYHPEHAGTPHQVQLGLLGVEYLKGRTFPAIAPPPSVESQCFRET
                     GHCIWGKFLERWRKLGLAVVGLPLSDQFEERGTDGKTYIVQYFERERFEYHPENQPPY
                     DVLLGLLGRQLYKP"
     gene            1412..>1529
                     /locus_tag="VFH60_03805"
     CDS             1412..>1529
                     /locus_tag="VFH60_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET6312936.1"
                     /translation="MLLGVLSDTHGTLHPRLLAVFREAGVERILHAGDVGKEN"
BASE COUNT          308 a          503 c          472 g          246 t
ORIGIN      
        1 gcgtgaaggt ctacgccgag atgctggcgg gcagctacgc agctatcaag caggccaacc
       61 cggaggccac cgtgctggta ggcggcctct ccatccacga ccgcaccaac gcgggcatgg
      121 ccttcctgga acaggtggtc gcggcctccg gcggcaagct caacttcgac gtgctctcca
      181 tccaccccta tatgcccgac cgccaccctg agagcaccga ccccaggacg gtcgtgcaga
      241 acttccccta ccgcctcgag atgagctaca agtggctgca agaccacgga gcgcccaaca
      301 aagaaatctg ggtgacggag acgggctatt cgacctgcaa cccgtgcggc aacctgggcg
      361 tgtctgagga ggagcaggca cggcggcttg tgaggctcta cgcgctcgca atggccgcgc
      421 ctaacgtcac ccacttcacc tacttccaga taaaggacaa gttcaaccac ccgcccggcg
      481 atatgttcgg caacatggct attatgcgca acgacatgag ccagaagccc gcatatatcg
      541 cctaccgggt catggcgcac acgcttgagg gtgccacctt caccgggcag gggccgctca
      601 tgagggccgt gcagaaccgc tggcagccac agttcgaccg ctaccattac aggttcacaa
      661 ggtcgggcaa gaacgtcagc gtaatctgga agctgggcca gccggagacc gtcaccatgc
      721 ccctcaccca gccgagcgtg gaggtcgtgt cttcgcgggg cgagaagctg gacgtgcagg
      781 tacacaacgg cgctatcacc ctgcgcatta gcgaagaccc catctacgtg gtcgagtcga
      841 accccaccac cagcgcggcg ctggaccccg ccaccgatgc tgcctccccc accggcttcc
      901 gggcctccct gcgcttccag ccctactggc agaacaacgg agggcttccc ttgttcggct
      961 tccccatcag cggcgagcgc aacgagacca acccgataga cggcaagcaa tacatcgtgc
     1021 agtggttcga acgcgcccgg ttcgagtacc acccggagca cgcgggcacg ccgcaccagg
     1081 tgcagcttgg cctgttgggc gtggagtacc tgaagggccg caccttcccg gcgattgcgc
     1141 ccccgcccag cgtcgaatcg cagtgcttca gggagactgg gcactgcatc tggggcaagt
     1201 tcctggagcg gtggcgcaag cttggcctgg cggtggtggg cctgcccctc tcggaccagt
     1261 tcgaggagcg cggcaccgat ggcaagacct acatcgtgca gtacttcgag cgggagcgtt
     1321 tcgagtacca cccggagaac cagccaccat atgacgtatt gctggggcta ttgggcaggc
     1381 aactgtacaa accctgagac ggaaagttga tatgctgcta ggcgtgctgt ctgacacaca
     1441 tgggacgctc caccctcggc tgctggctgt gttccgagag gcgggggtgg agcgcatctt
     1501 gcatgccggg gacgtgggca aggagaacg
//
LOCUS       DASRWZ010000242         1555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_19347,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000242 DASRWZ010000000
VERSION     DASRWZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19347"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1555
                     /locus_tag="VFH60_03810"
     CDS             <1..>1555
                     /locus_tag="VFH60_03810"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET6312937.1"
                     /translation="FDCSRINELRINEQMNLRAAAIMQHCSGQKSGQAGQKSNPVGSV
                     FSQLRSPLAYGGPDQDTIVGESSNLLQSESWVWSQGNTVVVTFTDFRGLNLSPRTLCG
                     ASTSTDGGATFTYLPVGVFSGLGNCSGDPFHYFSARANKWFAGFFSSACGANGIGQWE
                     SVDGINWTTSGCSHTGSSDDRFSGWVDNKVGSPFFGTQYVIFSNFALTNAPLQVVRST
                     DDGVTWSAAQTLSSRPGGGANRPVQLTGSLGTDGTVFAQSMDEGGGGLANRINYTHRT
                     TDGGATWTQIQQGPAFPAPGSGTCPANSFFAIIPGPWRHQGWGQPGVGPNNVAHYAYA
                     QHGAGADQGDIMYIRSTDNGSTWSSPIKLNTDATTRAQWMPSLSVNSQGSVFVGWYDR
                     RNTANNDYERFGRASTDNGVTWQADQAVSDVIIPQWAPDPNTAACYGGDYDYAMASDS
                     GQGTDHYQTWNDGRVVQGGVNQMNVFFDKIPLIGGTPTPTVTGTPPTNTPTRTATITS
                     TTTNTPVPSA"
BASE COUNT          310 a          554 c          438 g          253 t
ORIGIN      
        1 tttcgactgt tctcgcatca atgaactgcg tataaacgag cagatgaacc tgcgcgccgc
       61 cgccatcatg cagcattgca gcgggcagaa gtccggacag gccgggcaga agtccaatcc
      121 ggtcggctcg gtcttcagcc agcttcgcag ccccctggcc tacggcggcc cggaccaaga
      181 tacgatagtt ggcgagtcga gcaacctcct ccagagcgaa agctgggtat ggagccaggg
      241 caacacggtc gtcgttacct tcaccgactt ccggggcctc aacctcagcc cgcgcaccct
      301 ctgcggcgcg tctacctcaa ccgacggcgg cgcaacgttc acctaccttc ccgtcggcgt
      361 cttcagcggc ctgggtaact gctcaggcga ccccttccac tacttcagcg cgcgcgctaa
      421 caagtggttt gccgggttct tctcaagcgc ctgcggtgcc aacggcatcg ggcagtggga
      481 atcggtagac ggcatcaact ggacaaccag cggttgctcc cacaccggta gcagcgacga
      541 ccgcttctcc ggttgggtgg acaacaaggt gggcagccca ttcttcggaa cccaatacgt
      601 tatattctcc aatttcgcgc tcactaacgc gccgctacag gtggtcaggt ccaccgatga
      661 cggcgttacg tggagcgcgg cccagacgct ctccagccgc ccaggaggcg gcgctaaccg
      721 tcccgtgcag ttgacgggca gcctcggcac agacggcacg gtcttcgccc agtctatgga
      781 cgagggcggc ggcggactgg cgaaccgcat caactacacg catcgcacca ccgacggcgg
      841 ggccacctgg acgcagattc agcaagggcc tgccttccca gcaccaggtt cgggcacctg
      901 cccggccaac tccttctttg ccatcatccc cggcccctgg cggcaccagg gttggggcca
      961 gccgggcgtc ggccctaaca acgtcgcaca ttacgcttat gcccagcacg gcgcgggcgc
     1021 ggaccagggc gacatcatgt acattcgctc gaccgataac ggctcaacat ggtcgtctcc
     1081 catcaagctg aacacggacg ccaccacgcg ggcgcagtgg atgcccagcc tgtcggtcaa
     1141 ctcgcaaggc tcggtcttcg tgggctggta tgacaggcgc aacaccgcca acaacgatta
     1201 cgagcgattt ggccgcgcct cgaccgataa cggcgtcacc tggcaggccg atcaggccgt
     1261 cagcgacgta atcatcccgc agtgggcacc cgatcccaac accgcagcct gctatggcgg
     1321 cgattacgac tatgcaatgg caagcgacag cggccagggc actgaccact accagacctg
     1381 gaacgacggg cgcgtggtcc agggcggcgt caaccagatg aacgtcttct tcgacaagat
     1441 tcctctgatc ggcggcaccc ccacaccgac ggtgacgggc acccctccca ccaatacccc
     1501 cacccgcacc gctaccatta cctctaccac taccaacacc cccgtacctt ccgcc
//
LOCUS       DASRWZ010000243         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_773232, whole genome shotgun sequence.
ACCESSION   DASRWZ010000243 DASRWZ010000000
VERSION     DASRWZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773232"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..2174)
                     /locus_tag="VFH60_03815"
     CDS             complement(42..2174)
                     /locus_tag="VFH60_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6312938.1"
                     /translation="MSHSPAPAQRKYGMLLLCLLGTLAVALLLWPHNSLARPYSVARL
                     DGSASFYDKLSPLLAPVFGLNVRANSDDTDAGQHEPSLAVSRVDTNTVVIAAKDFRVD
                     NAKQVWIYSSTDGGATWPASRQLQMPGLPADVPNQSDPVAVGRDDGRIYVSALALNEP
                     DRTRHGLFITWTDDGGLTWRPSVPVYYPDTVLDDKDWFAIDNNPASPYYHYMYMAYVP
                     DGVDVVVQRSTDGGLTWSPRQQVGFGGTEYPYPVVGPDGAVYLFMMHNWGAGRTGNVQ
                     MTSSADGGATWTAPATIAQAVQPGNPIRTGDQFRFFATLAAAVDPNTGELYVAWTDRR
                     NITTNGVEVLYVKGTPGGGSWSAPARLSHDAPGVVRDHITPVLYADRNSRLHAFWLDR
                     RLDPDNRLFDSWYSSTTDGGATWDADTRVSTASQDLNVGLPPGSGNAAGDYWGLDVVS
                     DTVYVAWNDARSGQQDILVSRGTLRDDPGPTATPTVGTCSTEFADVAPGSTFYPFVRC
                     LACRNVLGGYPCGGEAEPCDGAARPYFRPGANISRGQISKVVSISAGFEEAPGGQVFE
                     DVPPGSTFYPWIQRLALRGIMSGYACGGEAEPCATTANRPYFRPSQPASRGQLSKIVA
                     NAAGASESVPPGTQTYADVPPSSPFWLYIERLSARNVIGGYPCGGAGEPCDGQTRPYF
                     RPEANVTRAQAAKIVANTFFPNCDAAAP"
     gene            complement(2203..>2658)
                     /locus_tag="VFH60_03820"
     CDS             complement(2203..>2658)
                     /locus_tag="VFH60_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312939.1"
                     /translation="TGSNLLLWDFRGCDAAVLMQWEVINASASCAPGKGLLLKPANED
                     PQVVNQALQIAPSQGGDRYVRVRTSFSVAAGAVTPESMTQLFWTDPTAYFSGFKKRQA
                     PLPPDGAPHVLWAFLAPHEVGQSITALRIDPINSTAQAEVRWIAVDTVR"
BASE COUNT          425 a          831 c          962 g          440 t
ORIGIN      
        1 ccaaagatac gcaaaactca gtatggacgc gcgtgttatg tctatggagc ggccgcatcg
       61 caattgggga agaaagtgtt ggcgactatc tttgcggcct gggcgcgcgt gacgttggcc
      121 tccggccgga agtagggccg cgtctgtcca tcgcatggct cacccgcccc gccgcagggg
      181 tacccgccta tcacattgcg ggcggaaagc cgctcgatat agagccagaa ggggctgcta
      241 ggcggcacgt cggcgtaggt ctgggtgccg gggggcacgc tctcgctcgc gcccgccgcg
      301 ttcgccacga tcttggatag ctgcccgcga gaggccggtt gcgatggccg gaagtagggg
      361 cggttcgctg tcgtggcgca aggctcggcc tccccgccgc acgcgtagcc gctcatgatg
      421 ccccgcagcg ccagccgctg tatccacggg tagaaagtcg agccgggggg cacgtcctcg
      481 aacacctggc ctccgggggc ctcctcgaag cccgccgaga tggacaccac cttgcttatc
      541 tgcccccggc tgatgttggc tcctggccgg aagtaggggc gggctgcccc gtcgcaaggc
      601 tcggcctcgc cgccgcacgg gtagccgccc agcacgttgc gacaagccag gcacctcacg
      661 aacgggtaga aggtgctgcc cggcgctacg tccgcgaact cggtggaaca ggtccccacg
      721 gtaggtgtgg cggtggggcc ggggtcgtcg cgcagggtgc ccctggacac gaggatgtcc
      781 tgctgccctg accgggcgtc gttccaggcg acgtacaccg tgtcggacac cacgtcgagg
      841 ccccaatagt cgcccgccgc gttgccgctg ccaggcggga ggcccacgtt caagtcctgc
      901 gaggcggtgc tcacgcgggt gtcggcgtcc catgtcgctc cgccgtcggt ggtagagcta
      961 taccagctat cgaacaggcg gttgtcgggg tcgaggcggc ggtcgagcca gaaggcgtgc
     1021 aggcggctgt tcctatcggc gtagagcacg ggggtaatat ggtcgcgcac cacgccgggt
     1081 gcatcgtggc tcaggcgggc gggcgcgctc caggacccgc cgccgggggt gcccttcacg
     1141 taaagcacct cgacgccatt ggtggtgatg ttgcggcggt cggtccaggc cacgtaaagc
     1201 tctccggtgt tggggtccac cgcggcggcg agggtggcga agaagcggaa ctggtcgccg
     1261 gtgcggatag ggttgccggg ctgcacggcc tgcgcaatcg tggcgggcgc agtccaggtc
     1321 gccccgccat cggccgagct ggtcatctgc acgttgccgg tgcgccccgc tccccagttg
     1381 tgcatcatga agaggtacac cgcgccgtcc ggccccacca ccgggtaagg gtactcggtg
     1441 ccgccgaagc ccacctgctg gcggggcgac caggtcagcc cgccatcggt ggagcgctgc
     1501 acgaccacgt ccacgccgtc gggcacgtaa gccatgtaca tgtagtggta gtacgggctg
     1561 gcgggattgt tgtcaatcgc gaaccagtcc ttgtcgtcca gcaccgtatc ggggtagtag
     1621 acgggcacgg agggccgcca ggtgaggccg ccatcgtcgg tccaggtgat gaagaggccg
     1681 tggcgggtgc ggtccggctc gttcagcgcg agggcagaga catagatacg gccgtcgtcg
     1741 cggcccacgg ctaccgggtc gctctggttg ggcacgtccg ccgggaggcc gggcatctgt
     1801 agctgccggg aagcgggcca cgtcgcccca ccatcggtcg aggaatatat ccacacctgc
     1861 ttggcgttat ctaccctgaa gtccttggcg gcgattacca ccgtattggt atcgacgcgg
     1921 ctcacggcca ggctcggctc gtgctgcccc gcgtccgtgt cgtcggagtt ggcccgcacg
     1981 ttgaggccga atacaggggc cagcagcggg ctaagcttgt cgtagaaaga cgcagagccg
     2041 tccaacctgg cgacagagta gggccgggcg agggagttgt gcggccagag caggagggcc
     2101 actgccagcg tacccaggag gcagaggagc agcatgccgt acttgcgttg ggcgggagcg
     2161 ggcgagtgag acaacaagag ggcctttcta tggggatggt agctagcgca cggtatcgac
     2221 ggctatccag cgcacctctg cctgggccgt cgagttgatg gggtctatgc gcaaggccgt
     2281 gatggactgc cctacctcgt ggggtgccag gaaagcccac aggacgtgtg gggcgccatc
     2341 gggaggcagg ggcgcttgac gtttcttgaa gccgctgaag taggcggtcg gatcggtcca
     2401 gaacagttgc gtcatactct ccggcgtaac cgcgcccgcc gcgaccgaga acgaggtacg
     2461 tacgcgcacg tagcggtcgc cgccctggga gggcgctatt tgcagggcct ggtttacgac
     2521 ctgtgggtct tcgttggcgg gcttgagcag cagccccttg ccgggcgcgc agcttgcgga
     2581 ggcgttgatt acctcccact gcatgagcac cgccgcatcg cagccccgga agtcccacag
     2641 cagcaggtta gagccggt
//
LOCUS       DASRWZ010000244         4059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1321624, whole genome shotgun sequence.
ACCESSION   DASRWZ010000244 DASRWZ010000000
VERSION     DASRWZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321624"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFH60_03825"
     CDS             <1..417
                     /locus_tag="VFH60_03825"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET6312940.1"
                     /translation="RGTEKLLAGVAHGAIAFGLFGVTFPVALLITGVIWLYSRRSPYV
                     RFHSDQAGCYQCSVLLINVLVVLLLLAFGGFAVVGVIRGETTLSELGPVLWGLGLFVA
                     WFAITIILGVIGAIMVLLGRPFKYPFIGDWFLKRGR"
     gene            746..3817
                     /locus_tag="VFH60_03830"
     CDS             746..3817
                     /locus_tag="VFH60_03830"
                     /inference="COORDINATES: protein motif:HMM:NF036537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LodA/GoxA family CTQ-dependent oxidase"
                     /protein_id="HET6312941.1"
                     /translation="MFVRMGEEGQPAVQPGGQAASAAEAAPDCGDDPIATLKHVFLKV
                     LMETRIAQGQDPALRPVFVKPHGVARGTFTMVPDLPHELRVGVFGHRSFPAWVRFSGD
                     TLPTNPDFNKAQGTGRTLGIGIKLFGVPGTKVLEPEVNAATHDFLLENYDLFFVDNVQ
                     EFCEFIYAGFVEGDFGKYLKDHQETQEILNELHKPVPSVLQTSYWSALPFAFGSERYV
                     KYKVVPRQVPDVGPGPAITNDDPNYLHADLRRRLLNGAAELDFYVQLRTGKMPLDAAM
                     TRWEESESKPIHVATLYMPQQDVEARGQAAYGEHLAFNTWHALPEHMPVGSIAQARRV
                     VYQAAAEFRRNVNGIPVGEPVEPRPLLFEPALRDEKIVRAAIHPAIGIARVGNSKTEY
                     FIGPEVPDPQPGPPGFYKDKKGALKRQAALFRVYGYNAAGEVVSELTADKADINWTVH
                     VANTKGAWYQFQIALDIPEAPSAHQTQLRNQDVTDRTQLVIDPGPRSIKGTNVSGDKY
                     AFNSGKFLGDTVYLGELRTDEQGRLIFLGGKGESASPSGGSITTFANNDGWYDDVSDG
                     PVTATVSIGGREVPVEGAWVVVAPPNYGTNVVDVRTMYDLLNDVNIAAGWTPLPKTVS
                     FKDDIYPILARFCNLQWVNQGFAVQFGWGGPNNFTDPEYVKQLAGTGAEYAELRQQIY
                     NSMRVLNRDGESPVPWPWIYGDAMNIPPVSPRQYMALSPTQMYMLEQWVNGKFEADWG
                     TGSEPPHSLDQVPLEQQPATLDQAALHFCLADAFHPGCEMTWPVRHSTMYSAPFRIKH
                     RPAGEPVPDYGLVLLPEVATGPTGPLYAQGPGDISRWMAIPWQADTASCRSAYTDSAY
                     GSNYDPYVPTFWPARVPNHVLTEINYEIVMDETRPREERIAAFRERASWFRVLKGDSL
                     AQKNQMVRDFGKMGVVEVREGIPNDPDFPPFIFVESRPGFDVEEIAPRRNLVMMHTPG
                     GAEGAPRAAADAAIEASGRPHEEVVTGYHDEVHRFVRRPFVLVPTSPDLEE"
     gene            3908..>4059
                     /locus_tag="VFH60_03835"
     CDS             3908..>4059
                     /locus_tag="VFH60_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6312942.1"
                     /translation="MYDVAVVGGGPAGAVAALGLARASRRVLLLDKSGAGEFKVGEGL
                     PPAAMPL"
BASE COUNT          751 a         1193 c         1379 g          736 t
ORIGIN      
        1 cgggggacgg agaagctctt ggcgggggtg gcgcacgggg caatcgcgtt cgggttgttc
       61 ggggtgacgt tcccggtcgc gctgttgata acgggggtca tctggctcta ttcgaggcgg
      121 tcgccgtacg tgcgcttcca ctccgaccag gcgggctgct accagtgctc ggtgctgctg
      181 ataaatgtac tggtggtgct gttgctgctc gcattcgggg gcttcgcggt cgtgggggtg
      241 atacggggcg agactacatt gagcgagtta ggtccggtgc tatggggcct cgggctgttc
      301 gtggcgtggt tcgccatcac cattatcctg ggggttatag gcgcgataat ggtgctgctg
      361 ggcaggccct tcaagtaccc cttcattggt gattggtttt tgaagcgggg gcggtgagac
      421 gtgttgcgtc cctggtggct accttgaggg ttattgggtt gcgttcaggc gcgggaagtc
      481 tcgaactgcg ttggactgga gtgcgtggca aaagcatagt tggtaaaagg acactttatc
      541 agtcgtacat atgttgctga aatataggtc gaacggcgtt gacaagcgaa tgtgcctgtg
      601 ctaccatctg tctgtcttgc caattgtaga cccaggcttt ccgccgtgcg cccgaagctt
      661 tatagacacg ggcagaagcg ggcatccctg gagacgaccc tatcgtgacg ctcaacttga
      721 ccggttcggg gagggaccag aggctatgtt tgtacgtatg ggagaggaag gtcaaccggc
      781 ggtacaacct ggcggccagg cggcttcggc ggcagaggcg gcccctgact gcggcgacga
      841 cccaatcgcc acgctgaagc acgtattctt gaaggtgctc atggagacac gtatcgccca
      901 ggggcaggac cccgcgttgc gcccggtgtt cgtgaagccg cacggcgtcg cccgtgggac
      961 ctttaccatg gtgcccgacc tgccgcacga gctacgagtg ggggtcttcg ggcacaggag
     1021 cttcccggcc tgggtgcgct tctccggtga caccctgccc acgaatcccg acttcaacaa
     1081 ggcgcagggt accggcagga cgctgggcat tggcatcaag ctgttcggcg tgcccggcac
     1141 gaaggtgctc gaaccggagg tgaacgccgc tacacacgac ttcctgttgg agaactatga
     1201 tctcttcttc gtggacaacg tacaggaatt ctgcgagttc atctacgcgg gcttcgtgga
     1261 aggggacttc ggcaagtatt tgaaggacca ccaggagacg caagagattc tgaacgaact
     1321 gcacaagccg gtgccgagcg tgctgcaaac atcgtattgg agcgcactgc ccttcgcgtt
     1381 cggctccgag cgttacgtca agtacaaagt ggtgccccgg caggtgccgg acgtggggcc
     1441 tgggcctgcg attacgaatg acgacccgaa ttacctgcac gcggacctcc ggcgcaggct
     1501 gctcaacggg gccgccgaac tcgacttcta cgtgcagttg cgcaccggca agatgcccct
     1561 cgacgcggcg atgacccgct gggaagagtc tgagagcaag cccatccacg tggcgacgtt
     1621 gtacatgccg caacaggacg tagaggcgcg cgggcaggcg gcctacggcg agcacctggc
     1681 gttcaatacc tggcacgcgc tgcctgagca catgccggtg ggcagcatcg cgcaggcgcg
     1741 cagggtcgtc taccaggccg ccgccgagtt ccgccgcaac gtcaacggca ttccggtggg
     1801 agagccggtc gagccacgcc cgttgctctt tgagccggcg ctcagggacg agaagatagt
     1861 gcgggcggct atccacccgg cgataggcat cgctcgcgtg ggcaacagca agacggagta
     1921 cttcattggg ccggaggtgc ccgaccccca gcctgggccg cccggcttct acaaggacaa
     1981 gaagggcgcg ctcaagcggc aggccgcgct tttccgggtc tacggctaca acgcggccgg
     2041 ggaggtcgtg tcggaactga ccgccgacaa ggccgatatc aactggactg tgcacgtggc
     2101 gaacacgaag ggggcgtggt accagttcca gatcgcgctg gacatcccgg aagcgccgtc
     2161 ggcgcaccag acgcagcttc gcaaccagga cgtgaccgac cgtacgcaac tggtaatcga
     2221 ccctggcccg cgcagcatca aggggaccaa cgtatcgggg gacaagtacg ctttcaacag
     2281 cggcaagttt ctcggcgaca ccgtatacct gggggagttg cgcacggacg agcaagggag
     2341 gctcatattc ctcggtggca agggcgaatc ggcttccccc agcggcggct cgatcaccac
     2401 cttcgccaac aacgacggct ggtacgacga cgtatccgac ggcccggtga cggccaccgt
     2461 atccatcggc gggcgggaag tgccggtcga gggggcctgg gtggtagtgg caccgcccaa
     2521 ctacggcacc aacgtggtgg acgtgcgcac catgtacgac ctgctgaacg atgtcaacat
     2581 tgcggccggc tggaccccgc tgcccaagac cgtgtcgttc aaggacgaca tatatccgat
     2641 cctggcgcgc ttctgcaacc tccaatgggt aaaccagggc ttcgcggtgc aatttggctg
     2701 gggcgggcca aacaacttca cagacccgga gtacgtcaag cagcttgccg gcacgggcgc
     2761 ggagtacgcg gagttgcggc agcagatata caattccatg cgcgtgctca accgggacgg
     2821 cgagtcgccg gtgccctggc cctggatata cggggacgcg atgaacatcc cgccggtgtc
     2881 gccacggcag tacatggccc tttctcccac ccagatgtat atgctggagc agtgggtgaa
     2941 cggcaagttc gaggcggatt ggggcacggg aagcgagccg ccgcatagcc tcgaccaggt
     3001 gccgttggag cagcaacccg cgacgctgga ccaggccgcg ctccactttt gcctggccga
     3061 tgcgttccac cccggctgcg agatgacctg gccggtgcgg cattccacga tgtattccgc
     3121 gcccttccgc atcaagcacc gcccggcggg cgagccggtg cccgactacg gcctggtgtt
     3181 gctccctgag gtagccaccg gccctaccgg ccctctctac gcgcaggggc ctggcgacat
     3241 ttcgcgctgg atggcgattc cgtggcaggc ggataccgcc agttgccggt ccgcctatac
     3301 ggactcggct tacggctcta attacgatcc ctacgtgccg acgttctggc ccgcccgcgt
     3361 gcccaaccac gtgctgaccg agataaacta cgagatcgtc atggacgaga cgagaccgcg
     3421 cgaggagcgc atcgcggcgt tcagggaaag ggctagctgg ttccgggtgc tgaaaggaga
     3481 cagcctggcg cagaagaacc agatggtgcg cgacttcggc aagatgggcg tggtggaggt
     3541 gcgggagggc atacctaacg accccgattt tccacccttc atcttcgtgg agtcgcggcc
     3601 cggcttcgac gtggaagaaa tcgcgccacg gcgcaacctg gtcatgatgc acacgccggg
     3661 gggcgctgaa ggcgcgccac gcgcagcggc cgatgcggcg atcgaggcca gcggtaggcc
     3721 ccacgaggag gtcgtgaccg ggtaccacga cgaggtccac cggttcgtgc ggcggccctt
     3781 cgttcttgtg cccaccagcc cggacctgga agagtagtgg ggaatttgga atttcggatt
     3841 tcgaatttcg gatttgattg aactcctact aaattcgaaa tccgaaattc caaattccaa
     3901 attcgtaatg tacgacgtag ctgtggtggg gggcgggccg gcgggggcgg tggcggcgct
     3961 ggggctggcg cgtgcgtctc gtcgggtgtt gctgctcgac aagagcgggg ctggggagtt
     4021 caaggttggc gaggggttgc cgcccgccgc gatgccgct
//
LOCUS       DASRWZ010000245         5372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_636257, whole genome shotgun sequence.
ACCESSION   DASRWZ010000245 DASRWZ010000000
VERSION     DASRWZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636257"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFH60_03840"
     CDS             complement(<1..743)
                     /locus_tag="VFH60_03840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET6312943.1"
                     /translation="MSNHIYYADNLPLLQSMPEASVDLIYIDPPFNTGKKQQRNSLQT
                     VRSENGNRTGFGGQRYETVNLATRAYADAFDDYLAFLQPRLLEAYRVLSPTGSLYFHI
                     DYREVHYCKLLLDGIFGRECFLNEIIWAYDYGARTTRKWPPKHNNILLYAKDPDRYTF
                     NADAVDRIPYMAPELVGPEKAARGKTPTSVWWHTIVPTNGKEKTGYPTQKPLGIMRRI
                     LLASSNPGDLVLDFFAGSGTTGAACLELG"
     gene            complement(1028..3154)
                     /locus_tag="VFH60_03845"
     CDS             complement(1028..3154)
                     /locus_tag="VFH60_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312944.1"
                     /translation="MDPIERESEFDHTLYTAEFVTAEPDEPLSSILERVEAALSRTPN
                     VVLILPRGSKAFHSTHDFLALGKLHSGRDVRVSLASPDPTIASLARVLGFYVESVPDD
                     HDYYEAPGSVTEEETEQPTSPLPLGAPRLPGPLADNPEWVLSPSGYVHPVPSTTSTWL
                     NHPSDPAHAQEGKEGKEGKDGSGASPSGANGSKPAHPHLSMPLSRSGVPPPRTKPRAT
                     GQLQPAIITDPSLPSITGDRFDAAVPLVSTTGSGRIKARRSDEEAAAYVKLDEQATVR
                     KIRRFSARRVVTGVLVLLLLALVGAGTYAWVYLPEGTVEITPLRKDLTGLQIEVPVLI
                     VPSAAGRAGLAAPLGQADTGVISTYSLTATQLTATIVEQNTAPATGSRQIAVGRSRGT
                     IIFTNSTPYPVTVPAMTTFTAANGVTVQVTQGGVVPATVFTPGATVSSLELEVEATVD
                     GPGGNVAAKQLQSTYKGLTYINPNPIEGGALHTLKVVTQQDIDALVADLNGKALDQGR
                     ALGLLLAQVPQGHELITQTFKFGGVESSSSLNADQDGEAVSVVLTTTVQAFSYNKGDV
                     QNAVVEAIRDQVAMMPVTVGPVLDESTIRYEPPTFQALDAAQGRVTYSTSASARVHFT
                     LTPTLKEQIRSLVTGKEIAKARSLISERYGEYVNASAIQANVLWLSIDKLPGDPERIT
                     IAEGTGAAYGPAPSSPSTRTDPRSQR"
     gene            3524..4057
                     /locus_tag="VFH60_03850"
     CDS             3524..4057
                     /locus_tag="VFH60_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6312945.1"
                     /translation="MEEREAVARLKRGDISGLEGLVLRYQVQAVQAAYLVVGDYAHAE
                     DIAQAAFLRAYERIASFDAARPFGPWFMRIVVNRAMSAAGGRQDLSLEVRAETEPDLP
                     SPDPGLQELLEAAETREERLAALEKLSPGQRAAVEMRYFLDMSAVEVSQQLAVPEGTV
                     RRRLHDARLRLRRLLTI"
     gene            4121..5041
                     /locus_tag="VFH60_03855"
     CDS             4121..5041
                     /locus_tag="VFH60_03855"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET6312946.1"
                     /translation="MSQQFVSRVLREHAQTNMTRRPNLWPGLSARLQAQGALASKETN
                     VSTRIAKRPLVVAGALVAAVLLAFVAATLVQPAPVEASQVLARAEQASNPKTSTINTF
                     HGVYVIHSLTHRELGQYSDGRADMWYQASPYLYMFKGVNKIAGEPDVNWFVGRDEQGI
                     YDYMTGERYRNHPASQFPVKPFIALDWRRLSQGDSLGSAAGTGDAQFSLFDLYDTKIT
                     STETVAGRQAYKLELTARPVDRTQTGARWPAHERVTMWVDKDVFLILRIQVYDINGAV
                     SSNESFESIEINQPIDPSVFDFSEPTGAER"
BASE COUNT          935 a         1582 c         1818 g         1037 t
ORIGIN      
        1 ctgcctagtt cgaggcacgc cgcgccggtg gtgccgctgc ccgcgaagaa gtccagcacc
       61 aggtcgccgg ggttggagga ggcgagcagg atgcggcgca tgatgccgag gggcttctgg
      121 gtggggtagc cggtcttttc cttgccgttg gtgggcacta tagtgtgcca ccagaccgag
      181 gtgggggtct tgccgcgcgc ggccttttca gggcctacca gttcgggggc catgtagggg
      241 atgcggtcta ccgcgtcggc gttgaaggtg tagcggtcgg ggtctttggc gtagagcagg
      301 atgttgttat gcttgggggg ccacttacgg gtggtgcgcg ccccgtagtc gtaggcccag
      361 atgatctcat tcaggaagca ctcgcgccca aaaatgccgt ccagcagcag cttgcaatag
      421 tgcacctccc ggtagtcgat gtggaagtag aggcttccgg tgggggagag caccctgtag
      481 gcttctagca ggcggggctg gaggaaggcc aggtaatcgt cgaaagcgtc ggcgtaggcg
      541 cgggtggcga ggttcaccgt ctcgtagcgc tgcccgccga agccggtgcg gttgccgttc
      601 tccgagcgca cggtttgcag ggagttgcgc tgctgtttct tgccggtgtt gaagggcggg
      661 tcgatgtaaa tgaggtccac agaggcctca ggcatgctct gtagcagggg caggttatcg
      721 gcgtagtaaa tgtggttgct cataactatc tgagtccgag tggggcgcag tctgcttcga
      781 tagcgggcct ctatttcaac attcaggacc aaatacccct tgtcatcctg agcgtagcga
      841 aggatcaggt gggtacgcga gagtcttcaa cctacaggat gctggtcgta agagacactt
      901 tgttgcccaa atagcaacgg ccaaacacgg gcggctgatc cttcgctacg ctcaggatga
      961 cagggggaac aggggcctgt ctcgtcaaca ttataccctt ccatcctggc acgctctgcc
     1021 gcccgcgtca cctctggctg cgggggtcgg tgcgcgtgct gggggaggag ggggccgggc
     1081 cgtaggcggc gccggtgcct tcggctatag tgatgcgttc ggggtcgccg ggcagcttgt
     1141 cgatgctgag ccacagcacg ttcgcctgga tggccgaagc gttcacgtac tcgccgtacc
     1201 gctcgctaat caggctgcgg gccttggcta tctccttgcc cgtgaccagg ctgcgtatct
     1261 gctccttgag cgtgggggtg agggtgaagt gcacgcgggc gctggccgac gtgctgtacg
     1321 tcacccgccc ctgtgccgcg tcgagcgcct ggaacgtggg cggctcgtag cgtatcgtgc
     1381 tctcatccag caccgggccg accgtcaccg gcatcatcgc aacctggtcg cgtatcgcct
     1441 cgaccaccgc gttctgcacg tcacccttgt tgtagctgaa cgcctgcacc gtagtcgtga
     1501 gcacaacgct taccgcctcg ccatcctggt ccgcgttcag gctggagctt gactcgaccc
     1561 cgccgaactt gaaggtctgg gtgatcaact cgtgcccctg cggcacctgc gcaagcagca
     1621 gacccagcgc ccggccctgg tccagcgcct tgccgttcag gtccgccacc agcgcgtcaa
     1681 tatcctgctg ggtgaccact ttcagcgtgt gcagcgcgcc gccctctatg gggttagggt
     1741 tgatgtacgt gaggcccttg tacgtgctct gtagctgctt ggcggccacg ttgcccccag
     1801 gcccatccac ggtggcttct acctcaagct caagcgagct aaccgtcgcg ccgggggtga
     1861 acacggtagc gggcacgacc ccaccttgcg tcacctgcac ggtcacgccg ttggccgcgg
     1921 tgaacgtagt catggcgggc accgtcaccg ggtagggcgt gctgttggtg aagatgatcg
     1981 taccccgcga gcggcccacg gcaatctgcc tgctgccggt ggcaggcgcg gtgttctgct
     2041 ccacgatcgt ggcggtcagt tgcgtggccg tgagcgagta ggtgctgatg acgcccgtgt
     2101 cggcctggcc cagcggtgca gccagcccgg cacgtcccgc cgcggagggc acaatcagca
     2161 ccgggacctc tatctgcaac ccggtgaggt ccttcctgag cggggttatc tccacagtgc
     2221 cttccggcag gtagacccac gcgtacgtgc cggcccccac cagcgcgagc aatagcagca
     2281 cgagcacgcc cgtgacgacc ctgcgcgcgc tgaaacgccg tatcttgcgc acggtggcct
     2341 gctcgtccag cttgacgtag gcggcggcct cttcgtccga gcgccgggcc ttgatgcgcc
     2401 ccgacccggt agtggatacg aggggcacgg ccgcgtcaaa cctgtcgccc gtaatcgacg
     2461 gcagcgaggg atcggtgatg atggccggtt gtagctgccc ggtggccctc ggcttcgtgc
     2521 gcggaggggg cacgcccgac ctggagagcg gcattgacag gtgcgggtgg gcgggcttgc
     2581 tgccgttcgc gccgctgggg cttgcgccgc tgccatcttt gccttctttg ccttctttgc
     2641 cttcctgggc gtgggcgggg tcgctggggt ggttcagcca ggtggaggtt gtgctgggca
     2701 cagggtgcac gtaccccgag ggcgaaagca cccactccgg gttgtcagcg agggggcctg
     2761 gcaggcgggg cgcgcccagc ggcaggggcg aggtaggctg ctccgtctcc tcctcggtca
     2821 ccgagcctgg ggcctcgtag tagtcgtggt catcgggcac gctctcgacg tagaagccga
     2881 gcacgcgggc caggctggca atcgtagggt cgggggaggc caaagacacg cgcacgtccc
     2941 gcccgctgtg cagcttgccg agcgccagga agtcgtgcgt cgagtggaac gccttgctgc
     3001 cacggggtaa gatgagcact acgttcggcg tgcgcgacaa cgcggcctcc accctctcaa
     3061 ggatggagga aagcggctcg tccggctcgg cggtcacgaa ctccgcggtg tatagcgtgt
     3121 ggtcgaactc gctttcgcgc tctatcgggt ccatggtcag tgaggagttg ttagttgtta
     3181 gttgttagtt gttagtaagg tgggtgggtg ctggtctatg tgatagcatg gttaaacggc
     3241 ccgcgtccgt gttggggcgg gtggtcccat tatatacaca agtggggtct ggttgtgtaa
     3301 tatccactaa tcgagagagt accccgaacg tttcgcccgc tgtgggggta gtatctctgg
     3361 aaggccttcc aaataccaat tctacttgaa aggactgcaa gacagggggt aaaagggtac
     3421 ttgcccgccg gtaacctggc ggggtccgcc tgtgcctctt tcccaccttc tcgcgggcct
     3481 ttcaaggaga ggcggtacaa catgggtgta agaggagatg cagatggagg agcgcgaggc
     3541 ggtcgccagg ctcaagcgcg gggacatcag cgggcttgaa ggcctggtgc tccgctacca
     3601 ggtgcaggcg gtgcaggcgg cttacctcgt ggtgggcgac tacgcgcacg ccgaggatat
     3661 agcccaggcc gccttcctcc gcgcttacga acgtatcgcc tctttcgatg ccgctcgccc
     3721 gttcggcccc tggttcatgc gcatcgtggt gaaccgggcg atgtcggcgg cgggcggcag
     3781 gcaagacctc tcgcttgagg tgcgggccga gacggagcct gacctgccca gccccgaccc
     3841 cggcttgcag gagctgctgg aagcggcaga gaccagggag gagagacttg ccgccctgga
     3901 gaagctgtcg ccggggcagc gggccgccgt ggagatgcgc tactttctcg acatgagcgc
     3961 ggtcgaggta tcgcaacagc tagcggtgcc cgaaggcacg gtcaggcggc gcttgcacga
     4021 tgcccgccta cgcttgcgca ggctgctaac aatataagtc cgagcatgta ggggcgcggt
     4081 gcgtcgtacc cctgccgaaa gtgtaaatgg gagaacagct atgagtcagc aattcgtcag
     4141 ccgtgtgttg agagaacatg cgcagacaaa catgacgcgg cggcccaacc tgtggcccgg
     4201 cctcagcgcg aggttgcagg cccagggggc gctcgcgtcg aaggagacta acgtgagcac
     4261 tcgtattgca aagcggccgc tggttgtggc cggggcgctg gtagctgccg tactgctggc
     4321 ctttgtcgcc gcgaccctcg tccagcccgc gcccgtggag gcttcgcagg tgctcgccag
     4381 ggccgagcaa gcgagcaatc ccaagacctc gaccataaat accttccacg gtgtgtacgt
     4441 catccactcc ctcacccacc gcgaattggg ccaatactcc gatgggcgcg cggatatgtg
     4501 gtaccaggca tcgccgtacc tttacatgtt caagggcgtg aacaagatag ccggagagcc
     4561 ggacgtgaat tggttcgtgg ggcgggacga gcagggcatc tacgactata tgacgggtga
     4621 gcgctaccgt aatcacccgg cttcccagtt ccccgtaaag ccttttatcg cgctggattg
     4681 gaggagacta tcccaggggg atagcttggg atcggcggca ggcaccggcg acgcgcaatt
     4741 cagcctgttc gacctctacg acaccaagat aacgagcacc gagacggtag cgggccgcca
     4801 ggcatacaag ctggaattga cggcacgccc ggtagaccgc acccagaccg gggcacgctg
     4861 gcccgcccac gaacgtgtga cgatgtgggt ggacaaagat gtattcctca tcctgcgcat
     4921 ccaggtgtac gatatcaatg gcgccgttag ctcgaatgag agcttcgaga gcatcgagat
     4981 caaccagcca atagacccct cggtgttcga cttctccgag ccaacgggag cggaaaggta
     5041 acgcaagaaa ggcggaaccg ccaagacgcc aagaacgcca agagcgcgac aggattaagg
     5101 atggtgcgtt cttaaggaga gtaccagggt acgctagcac gtaccctggt cctttttgta
     5161 ctccccaacc cttcactctc tttgcgtgtt ctttgcgtct ttgcgtcttt gcgttttcgt
     5221 ctcttctcta aatgtccttg cgcaccccga acagaattgg ggtctgagag ctattattag
     5281 gcagaaggga ccttcaacca aaaatcgacc ggcggagggg gcatggaaga cgaacaagct
     5341 gttgcgcggc ttaagcgcgg cgacataggc gg
//
LOCUS       DASRWZ010000246         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_499226, whole genome shotgun sequence.
ACCESSION   DASRWZ010000246 DASRWZ010000000
VERSION     DASRWZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499226"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..2196
                     /locus_tag="VFH60_03860"
     CDS             277..2196
                     /locus_tag="VFH60_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6312947.1"
                     /translation="MDTFVGRRLGKYHLESLLGRGGMATVYRAHDTVLNRAVAIKVLD
                     PALTIDPRAVERFKREAVTAANLEHPNIVRVYDVQQDGNLHYISMRYVQGTTLREILQ
                     DNGPLPVETAVNIIVPVAQALHYAHKNGVIHRDVKPGNILVEPDGSVMLSDFGIARAA
                     GAAQAGLTATGLVMGTADYLSPEQISGRPAEPRSDIYSLGVVLYELLTGVTPFSGENT
                     ASVLYRQVHDTPPPLRSMNPRLPQELQPIIDRALAKNPSLRYSDALDLARDLEEVARW
                     LPQGSPWLKNNAGVNGTGSTTRYVPRSAAGNGNEAQPWDYGSQQAAVPYLEEADEPYE
                     VYEQFDPHVAYTRTPQPTGGGRRTMLLLFALLLVGLGAVVMAAGAGILPLKLGQGGRG
                     DAGGPTRFQAPGPVTYSGPVRTGNGPDIRVPMSLVPPTIDGDLSDWAGAEPWAVPAFD
                     SITYINDKVRDLKWGGPDDLSATFNFAWDDTNFYVATVVTDDVHMQNANTRGYNLYFG
                     DDIEIWFDLDLEGDFAQGTGNADDFQLGLSPGDFAANGPEAVFWNPDRKADRNKMVTV
                     RAQRRPANNGYVIEAAVPWTAFGDFRPQTGMGIGFVASAGDNDNPDVPRQELMISTGN
                     RLQYKQPLTFGMLYL"
     gene            2208..3035
                     /locus_tag="VFH60_03865"
     CDS             2208..3035
                     /locus_tag="VFH60_03865"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6312948.1"
                     /translation="MSSISDQGYLLSSQYKTAANLNARIRLHELYSTNKYGWFRWLFD
                     QFDLPDEAALLEVGCGPGTLWVENLSRVPEGWDVSLTDLSPGMVEEARANLQGRERGF
                     SFRQADAMSIPYPDETFDAVLAMYMLYHVPDRGKAFAEMRRVLKQDGKLYVATLGRMH
                     MYEVYELLNRFDAQAAARLPFGPPNPQAQEHRLGFILETAGEEIAQHFSQVELRRYED
                     ALKVTEAAPLVDYILSSSGSVFGQEQTQALTAFIQGELDAKGPIHIRKDNGLFVAQQ"
BASE COUNT          597 a         1076 c          909 g          492 t
ORIGIN      
        1 cctgtggctg aagccacggg ctgagaagag gcaaagtccc caacggggac tctcctacat
       61 gaaccagtct ccgaaggaga cttcgctact tcccagcccg tggcttcagc cacagggcga
      121 ccatgcgggg ctacgtacag ctaccatccc tgacgcgaga cgtgcaaacg ctggtaggca
      181 tgcggcaata agccgtataa tatcgctggt gccgctcgcg cgccttgcgt ctcctgcgcg
      241 ccttgattac caaccaccgg cacccaacaa ctcacattgg atacattcgt aggccgtaga
      301 ctaggcaagt accatctgga gtccctcctt ggcaggggcg gcatggctac ggtctacagg
      361 gcgcacgaca cggtgctcaa ccgcgccgtc gccatcaagg tgctcgaccc cgcgctcacc
      421 atcgacccca gggcggtaga gcgtttcaag cgggaagcgg tcaccgccgc caacctcgaa
      481 caccccaaca tcgtccgcgt gtacgacgtg cagcaggacg gcaacctcca ctacatctcg
      541 atgcgctacg tgcagggcac caccttgcgc gaaatcctcc aggacaacgg ccccctgccc
      601 gtcgagaccg ccgtcaacat catcgtaccc gtggcccagg ccctccatta cgcccacaag
      661 aacggcgtca tccaccgcga cgtaaagccg ggcaacatcc tggtggagcc ggatggctcg
      721 gtcatgctct cggacttcgg catcgcgcgc gcggcgggtg cggcccaggc cgggctgacc
      781 gctacggggc tggtgatggg caccgccgat tacctgtcgc cggagcaaat tagcggccgc
      841 ccggcggaac cgcgctccga catctactcc ctcggcgtgg tgctgtacga gttgctcacc
      901 ggcgtgacgc ccttctccgg cgagaacacc gccagcgtcc tttaccggca agtgcacgac
      961 actcctcctc ccctccgctc gatgaaccca cgcctgcccc aggagctaca gcccataatc
     1021 gaccgcgctc tcgctaagaa ccccagcctg cgctactccg acgccctcga cctggcccgc
     1081 gacctggaag aagtggcccg ctggctccca cagggcagcc catggctcaa gaataatgcg
     1141 ggcgtcaacg gcacgggcag caccacgcgc tacgtgcccc gctcggccgc gggcaacggc
     1201 aacgaagcgc agccgtggga ctacggcagc cagcaggcgg cagtccccta cctggaagag
     1261 gccgacgagc cgtacgaggt ctacgagcag ttcgaccccc acgtagccta cacgcgcacg
     1321 ccgcagccca cgggcggagg acgccgcacc atgctgctgt tgttcgcgct gctgctcgtg
     1381 ggcctgggcg ctgtggtcat ggcggcgggg gcgggcatat tgcctctcaa gttgggacag
     1441 ggcgggcgcg gtgatgcggg cggccctacg cgcttccagg ctccaggccc cgtcacctat
     1501 agcggccccg tgcgcacagg caatggcccc gacattcgcg tgcctatgtc ccttgtgccg
     1561 cccaccatcg acggcgacct ctccgattgg gcgggcgcgg agccgtgggc cgtcccggcg
     1621 tttgacagca ttacctatat aaatgacaag gtgcgcgacc tgaagtgggg cggcccggac
     1681 gacctgagcg ctaccttcaa tttcgcctgg gacgatacca acttctacgt cgccacagtg
     1741 gtcaccgacg acgtacacat gcagaacgcc aacacgcgcg gctacaacct ctatttcggc
     1801 gacgacatag agatctggtt cgacctggac ctggaaggcg atttcgcgca gggcaccggc
     1861 aacgccgacg acttccagct tggtctctcc cccggcgact tcgcggcaaa cggcccggag
     1921 gcagtcttct ggaaccccga ccgcaaggcc gaccgcaaca agatggtcac ggtgcgcgcc
     1981 cagcgcaggc ccgccaacaa cggctacgtg atcgaggccg ccgtgccctg gaccgccttc
     2041 ggggacttcc gcccgcagac cggcatgggc ataggcttcg tggcctcggc cggcgacaac
     2101 gacaaccccg acgtgccccg ccaggagctg atgatctcca ccggcaaccg cctccaatac
     2161 aagcaacccc tcaccttcgg catgctctac ctgtaacctt ttcaattatg tcaagcatat
     2221 ctgaccaggg ctacctcctg tccagccagt acaagaccgc tgcgaacctc aacgctcgca
     2281 tacgcctgca cgagctttac agcaccaaca agtacggctg gttccgctgg ctgttcgacc
     2341 agttcgacct tcccgacgag gcggcactac tggaagtggg ctgcggcccc ggcactttgt
     2401 gggtggagaa cctgtcgcgt gtgccggaag gttgggacgt atcgctgacc gacctctcgc
     2461 ccgggatggt cgaggaggcg cgggccaacc tgcaaggccg cgagcgaggg ttcagcttcc
     2521 gccaggccga cgccatgtcc atcccatacc cggacgagac cttcgacgcg gtgctggcga
     2581 tgtacatgct ctaccacgtg cccgaccggg gcaaggcgtt tgccgaaatg aggcgggtgc
     2641 tcaagcagga cggcaagctg tacgtggcga cgcttggccg catgcatatg tacgaggtgt
     2701 acgagctgct gaatcgtttc gacgcacaag cagcggcgcg cctccccttc ggccctccca
     2761 acccccaggc acaggagcac aggctcggct tcatactgga gactgccggt gaggaaatcg
     2821 cccagcactt cagccaggtc gaactgcgac gctacgaaga cgcgctgaag gtgacggaag
     2881 ccgcgcccct ggtagattac atactctcca gttcaggctc cgtcttcggc caggagcaga
     2941 cccaggcgct gaccgccttc atccagggcg agttggacgc taaaggcccc atccacattc
     3001 gcaaagacaa cggtctgttc gtagcgcagc agtagacgct ctgccgcagg gcagtcttaa
     3061 agactcccgg taca
//
LOCUS       DASRWZ010000247         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1321750, whole genome shotgun sequence.
ACCESSION   DASRWZ010000247 DASRWZ010000000
VERSION     DASRWZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321750"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1047
                     /locus_tag="VFH60_03870"
     CDS             205..1047
                     /locus_tag="VFH60_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6312949.1"
                     /translation="METETRIRVTALASGSSGNAFLIEAAGTRLLFDAGLPAATLEHY
                     LRQCGTSPSQLSAIFVSHEHIDHLRGAGMLARRYRLPVVASEGTFLAGAMQLGSLPEK
                     LVQPPGREVYVGTDGADGVVVRTFAVSHDAAEPVGFWIEAGGYNIVLCTDLGCETASI
                     RDALEAADLLVIEANHDVQRLWRGPYPPRLKRRVAGRHGHLANDDAARLVSGLARDAR
                     PRTVWLAHLSATNNTPALAFDTVAQPLDREGCTHLEVAVLARDKPSHVWQGAASPGEV
                     KATA"
     gene            complement(1400..2101)
                     /locus_tag="VFH60_03875"
     CDS             complement(1400..2101)
                     /locus_tag="VFH60_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6312950.1"
                     /translation="MSLVRLVGLTRQYRMGPNLVAAVDGVDLEIGAGEAVALVGPSGS
                     GKSTLLNLIGGLDRPTSGEVWVDGENIARAPARRLVEHRRMRIGFIFQSFNLLSHRTA
                     LENVEVPLMLAGLPRKERQARARELLTRMGLGPRTGHRPSQLSGGEQQRVAIARALAN
                     QPAILLADEPTGNLDSATGAEVMSLLRELKSGGLTIVLVTHDMAVASYMDRIVRLRDG
                     KIVSIESTADVATPG"
BASE COUNT          407 a          778 c          723 g          437 t
ORIGIN      
        1 gccctgccaa catcgccctg tggctaaagc cacgggcttg ccagagacga agtctcctac
       61 ggagactaca tgatgttcct tagtccccgt aggggacttt gcctcttgta agcccgccac
      121 tttagtggca gggcacaccc acagggcacc ccaacagggc acccccctct tgacatcagc
      181 gaacatttgt actattgtta gctcatggaa acggagacgc gaatacgggt cactgccctt
      241 gccagtggca gttcaggcaa tgccttcctc atcgaagcgg cgggcactag gctgctgttc
      301 gacgccgggc tgcccgcggc gacattggag cactatctgc ggcagtgcgg cacctcgccc
      361 tcgcagttgt cggccatatt cgtcagccac gagcatatcg accacctgcg cggcgccggt
      421 atgctggcgc ggcgctacag gctgcccgtg gtggctagcg aaggcacgtt cctggccggg
      481 gcaatgcagc ttgggtcgct ccctgagaag ctcgtgcagc cgcccggacg ggaggtatac
      541 gtgggcacgg acggtgcgga cggggtggtc gtgcgcacct tcgcggtctc ccacgacgcc
      601 gccgagcctg taggtttctg gatcgaggcc gggggctaca atatcgtgct gtgcaccgac
      661 ctcggctgcg agacggcgtc catacgcgac gcattggagg ccgccgacct gctggtgatc
      721 gaagccaacc acgacgtgca acgcctctgg cgcggcccct atcctcccag gctcaagagg
      781 cgcgtagcag gccgccacgg ccacctggcc aacgacgatg ccgcccgcct ggtctcagga
      841 ttggcccgcg acgcccgccc gcgcaccgtc tggctggccc acctctccgc caccaacaac
      901 acccccgccc tggccttcga tacggtcgcc caaccattgg accgcgaggg ctgcacccac
      961 ctggaagtcg ccgtcctggc gagagacaag cccagccatg tctggcaagg tgccgcgtct
     1021 cccggcgagg tcaaggcaac ggcataagtc cgcgcccgcg ccctgtcatc ctgagcctcc
     1081 cctctgtcat cctgagcgta gcgacggatc aggtgggtag gcgagagtct acaacgctga
     1141 ggatgctggc ggcttgagga acagtggtgc tccggtagca agagtctaac acgggcggct
     1201 gatccttcgc ggagtttacc ctgggcgtag ccgaagggct ccggatgaca aggccactct
     1261 tggtcctacc ggtccaacat gggggttgag caccagtgtg cggtccaccg tacccctaca
     1321 caccgcaaac tatcctaaac tttgcgcgta ctttgcgtgc tttgcgtctt tgcgttttcg
     1381 tctctttacc agctacgccc taccccggtg tggcaacgtc cgccgtgctc tcgatgctga
     1441 ctatcttgcc gtcgcgcagg cgcacgatgc ggtccatgta ggaggcgaca gccatatcat
     1501 gggtgacgag cacgatggta aggccgcccg attttagctc gcgcagcaac gacatgacct
     1561 cggccccggt ggcgctgtcg aggttgcccg taggctcgtc ggccaggagg atggcgggtt
     1621 ggttggcgag ggcgcgggcg attgccacgc gctgctgctc gccgccggat agctgtgagg
     1681 gacggtggcc ggtgcgcggg ccgagtccca tgcgggtaag tagctcgcga gcgcgggcct
     1741 gcctctcctt gcggggtagc cccgccagca tcaggggcac ttcgacgttc tccagcgcgg
     1801 tgcggtgcga cagcaggttg aagctctgga agatgaagcc tatgcgcatg cgccggtgtt
     1861 cgaccagccg ccgggccggt gcgcgggcga tgttctcccc atccacccac acctcgcccg
     1921 aagtcggcct gtcaagccct cctatcaggt tgagcagcgt ggacttgccc gaaccggacg
     1981 gccccaccag cgccaccgcc tcccccgccc cgatctccag gtccacccca tccaccgccg
     2041 ctacaagatt aggccccatc cggtactgcc gggtgaggcc cacaagccgg actaacgaca
     2101 tggcgacgaa tttggaaatt ggaaattcga atttcggatt tgacaggagt tcttctaaat
     2161 ccgaattcga atttccaatt tccaaattac ttcagcccct ctgcgatctt ggcggcttct
     2221 tctactgtga agttgccgcg tacggcgagg aagaggccgg tgccttgctc ctgccaggtg
     2281 agcacggtcc agttgccgcc ttcgggagag aacgcattgg ctttcgcgcc gcgcacggta
     2341 agttc
//
LOCUS       DASRWZ010000248         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1184740, whole genome shotgun sequence.
ACCESSION   DASRWZ010000248 DASRWZ010000000
VERSION     DASRWZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184740"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..583
                     /locus_tag="VFH60_03880"
     CDS             119..583
                     /locus_tag="VFH60_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312951.1"
                     /translation="MVASSGSDGGVEEASEATYMEMLAKREDQDLLDVSRRLLDWAQG
                     EGLPLHWSSVRDVPLFAPAIETPTGPAYFVEVTARGRIDVLFDRLRETDAFRDDAQRL
                     EVLRRLNGIPGMGMPEFTIDKRPTFSLNALVSDEAFEQFTSTFRWVAQQVRG"
     gene            complement(1106..>2654)
                     /locus_tag="VFH60_03885"
     CDS             complement(1106..>2654)
                     /locus_tag="VFH60_03885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET6312952.1"
                     /translation="RLADMFGYELHEVVGMNMWSFTPFEYQSMILDKALIDDETPYEL
                     VGVRKDGSTFPIEVHGQTVPYQGRTVRVTTTRDLTEQKHAEQVLKQSEREYRGLFENA
                     HDAILIIAPEDEIVLDANAAACKLYGFTREEFAGMSILRVSGDPERISGPNARRLEDG
                     GTIQFETVQYRKDGTEIALEITAAMVEYKGQPAYLSINRDVTERKRAEEALRASEQKY
                     RLLMEGATDSVVIIDREGNYIEVNDMACRALGYTRDEFLTLNMHQVIAPEDLSARPLM
                     DDRRAARKGLLFERTMLRKDGTRMLVEISARLLDDGSVQAIVRDITERKRAEEALHVL
                     ATQDALTGLLNRREMDRVLEEEVTRCRRYERTLSVVMIDIDHFKSVNDRYGHKVGDEV
                     LRWMGGIFRNSVRSTDRVARYGGEEVLAILPETGEMEAYRVAERFRATVAARPFTYTT
                     PDGEAVSLPITISLGVAELPGHALTADSLVIAADNALYHAKRSGRNRVVSYTLSALAT
                     TAEIAAR"
BASE COUNT          466 a          771 c          879 g          538 t
ORIGIN      
        1 gacggacatc taccaaaagg tagacatacg ggctggactc tgggcgcggg caccaggcct
       61 gataatatcg tcagtgttat attgtccaca tagatcaagc gaaggagcgt tcaaggagat
      121 ggttgcaagc agcggctcgg atggcggggt ggaggaggcg agtgaggcta cgtacatgga
      181 gatgctggcg aagcgcgagg accaggacct gctggacgtg tcgcggcggc tgctcgactg
      241 ggcgcaaggc gagggactgc ccctccactg gagtagcgtg cgcgacgtgc ccctgttcgc
      301 gcctgctata gagacgccaa ccggccccgc ttacttcgtg gaggtcaccg ccaggggcag
      361 gatagacgtg ctgttcgacc ggctgcgcga gacggatgcc tttagagacg acgcgcagcg
      421 gctggaagtg ctgcgccgcc tgaacggcat acccggcatg ggtatgcccg aattcaccat
      481 cgacaagcgg cccaccttct ctctcaacgc cctcgtcagc gacgaagcgt tcgagcagtt
      541 cacctcgacc ttccggtggg tagcgcaaca ggtgaggggc tagcagccgt cacgtcccca
      601 ctgctacagc acccaagctc ccactgtcat gccaatggtg catcttgcaa gcaagggtca
      661 ataacaggac gcaaagtggt ctttgtcatc ctgagagggt gtctaataat ggcagagggt
      721 agatttaggc acaaaaacaa ggacatacag gttggaagga gcgcaggaga aagcaaccgt
      781 tgtgaccata gagcctattc gagaggcatt ttccttcgtg atgctcttgt ccttcactct
      841 tcccttgcac tccgtactat attattagac accctctgag cgtagcgaag gatcaggtgg
      901 cagtcggcct ttgtggcaat gcagacatct ggcggcaagc cggcgtgggt tctccaatac
      961 agcttcttcc ctacccacct gatccttcgc tacgctcagg atgacaatgc cgcgccttgg
     1021 tgctgtaaaa gagagagcgg aacgtgagtg ttgcaagaaa cccatgtagt gacctgtgcc
     1081 gaaggcaagg tttggggtgt agggcttagc gggcggctat ctcggcggtg gttgcgaggg
     1141 cggagagggt gtagctgacc acgcggttgc ggcccgaccg cttggcgtgg tagagggcgt
     1201 tgtcggctgc tatcacgagg gagtctgcgg tgagggcgtg gccgggtagc tcggctacac
     1261 ccaggctgat ggtgatgggc aggctcacgg cctcgccgtc gggcgtggtg taggtgaagg
     1321 ggcgggcggc cacggtggcg cggaagcgtt cggccacgcg gtaggcctcc atttcgccgg
     1381 tttcgggcag gatcgccagg acttcctcgc cgccgtagcg ggccacgcgg tcggtcgagc
     1441 gcacgctgtt gcggaagata ccgcccatcc agcgtaatac ctcgtcgcct accttgtgcc
     1501 cgtacctgtc gttcaccgac ttgaagtggt cgatgtcgat catgaccacc gacagggtgc
     1561 gctcgtagcg gcggcagcgg gtgacttcct cttcgagcac gcggtccatc tcgcggcggt
     1621 tgaggaggcc ggtcagggcg tcttgcgtgg ccagcacgtg caaagcttcc tcggcgcgct
     1681 tgcgctcggt gatgtcgcgc acaatggcct gcaccgagcc gtcgtccagc agccgggcgc
     1741 tgatctccac cagcatgcgc gtgccgtctt tgcgcagcat ggtgcgctcg aagagcaggc
     1801 ccttcctggc ggcgcggcgg tcgtccatca gtgggcgggc ggaaaggtcc tcgggcgcta
     1861 tcacctggtg catattgagc gtgaggaact cgtcgcgggt gtagcccagc gccctgcacg
     1921 ccatgtcgtt gacctctatg tagttgccct cgcggtctat gatgaccacg ctgtcggtgg
     1981 ccccttccat cagcaggcgg tacttctgct cgctggcccg gagcgcctct tcggctcgct
     2041 tgcgctcggt cacgtcgcgg ttgatgctca agtaggcggg ctggcccttg tactcgacca
     2101 tcgcggcggt gatttcgagg gcgatctcag tgccgtcctt gcggtactgc accgtctcga
     2161 actgtatcgt gccgccatcc tccaaccgcc tggcgttggg gccggagatg cgttcggggt
     2221 ccccggaaac gcggagaatg gacataccgg cgaactcttc gcgcgtgaag ccgtaaagct
     2281 tgcacgctgc ggcattggcg tcgagcacta tctcatcttc gggagctatt atcaggatgg
     2341 cgtcgtgggc gttctcgaac aggcccctgt actcgcgctc cgactgtttt aggacttgct
     2401 cggcatgctt ctgctcggtc aggtcgcggg tggtggtgac gcgcaccgtg cggccctggt
     2461 agggcaccgt ctggccgtgg acctctatcg ggaaggtaga gccgtccttg cgtacgccca
     2521 ccagctcgta gggggtctcg tcgtcgatga gcgccttgtc caggatcatg ctctggtact
     2581 cgaacggggt gaaggaccac atgttcatgc cgaccacttc gtgcaattcg tagccgaaca
     2641 tgtccgccag gcgc
//
LOCUS       DASRWZ010000249         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_636434, whole genome shotgun sequence.
ACCESSION   DASRWZ010000249 DASRWZ010000000
VERSION     DASRWZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636434"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1303)
                     /locus_tag="VFH60_03890"
     CDS             complement(221..1303)
                     /locus_tag="VFH60_03890"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HET6312953.1"
                     /translation="MNQYDIALIPGDGIGNEVIPAGLKVLQAAGELTGSFRLNTRHFP
                     WGCEFYLQHGRMMDPDGLKQLEPFEAIYLGAVGYPTVPDHISLWGLLLPIRQGFDQYI
                     NLRPIRLLEGIPGPLRDKGPKEINFVVVRENSEGEYSGHGGRSHLGRPQEVATQTTVF
                     TRTGVARVVRYAFELARSRGPRPGAPFISATKSNAQQYTSVLWDEVCAEVAAGYPEIE
                     WRSMLVDALAALMVLHPERLDVVVASNLFGDILTDLGGALQGSLGVPASANLNPERHY
                     PSMFEPVHGSAPDIAGRGLSNPLATIWAGSMMLDHLGEPEAARLVMRAMEQVVARGET
                     LTPDFGGTATTGQVADAVIAALGKDA"
     gene            1763..3898
                     /locus_tag="VFH60_03895"
     CDS             1763..3898
                     /locus_tag="VFH60_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6312954.1"
                     /translation="MTTPTPAYLDTTRPLTERVTDLVSQLTLEEKLSQLQNSTPGVPR
                     LGIPAYNYWNEALHGVARNGRATIFPQAIGMAATWDPPLIQRVASDIGDEARAKYHEA
                     LRRNGFTRLYQGLNFWSPNVNIYRDPRWGRGQETWGEDPYLTGQMGAAFVRGMQGDHP
                     RYLKAAACAKHYAVHSGPEKQRHGFDAHASLRDMHETYLPAFKALVDAGVESVMGAYN
                     RTNGEPCCAHSYLIGEVLRGQWGFQGHFLSDCGAIDDIYRGHGAAPDAASAAALALKR
                     GCDLECGGTYRHLGEALERGLVTMDDIDRALSRVLATRFKLGMFDPPEQVPYASTPLS
                     VVGSEAHRALSYEAAVKSVVLLKNRNNILPLSPDVRTMRVLGPTAVDVSVLLGSYHGL
                     NGTLTTLLEGIVSRMPEGVGMEYRMGVQLIHPNTASQEWLYSPRSQPDVVIACMGVTP
                     MLEGEEGDSIASTEEGDRAGISLPSVQAEFVRKLAGAGAKVVLVLTGGGPIALGDLEE
                     LVEAVVFVWYPGEAGGLAVADVLFGHVTPSGKLPLTFPRSLDDLPPFDDYGMQGRTYR
                     YADKEPLYPFGFGLSYTRFAYSDLQMEREQIAAGDELAVSVTLTNSGNVDGEEVVQVY
                     ASALDVPGAPISSLVAFRRVALRAGERKSLAFEVPAERLALVDEEGIARVVPGRMRLT
                     VGGCSPGERGLNLGAARPASGEFLILGHI"
     gene            4141..>4420
                     /locus_tag="VFH60_03900"
     CDS             4141..>4420
                     /locus_tag="VFH60_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312955.1"
                     /translation="MNEAQADSSVQTDGGHAYEAGTSDEARQEALAPVTQQPVSPADE
                     VATRAIDGSAAEARGSGKPRSGIALCLSGGGYRAMLFHLGALWRLNELG"
BASE COUNT          789 a         1376 c         1492 g          763 t
ORIGIN      
        1 atcctgagcg cagcgaagga tcagccgccc gttcttgact ctccctactt gagcaccaac
       61 gttcctctta ccagcattat cctacacgtt gtagactctc gcctacccaa ctgatccttc
      121 gctacgctca ggatgacaaa ggggagctca ggatgacgag ggtgggtttt ggccgcctac
      181 acgtaaattg gagcttcgtc ccctgttagc tcatggtgat tcaggcgtcc ttgcccagcg
      241 cggctattac cgcatcggct acctggccgg tggtggccgt gcctccgaag tcgggggtga
      301 gagtttcgcc acgggctacc acctgctcca tagcgcgcat caccaggcgg gcggcttcag
      361 gctcgcccag gtggtccagc atcatggagc ctgcccagat ggtggctagc gggtttgaga
      421 ggcccctgcc cgcgatgtcg ggggcggagc cgtgcaccgg ctcgaacatg gaggggtagt
      481 gccgctccgg gttgaggttg gcgctcgccg gtacccccag actgccttgc aaagcccctc
      541 ccaggtcggt cagaatgtcg ccaaagaggt tggaggctac aaccacgtcg aggcgttcgg
      601 ggtggagcac catcaaggcg gccagcgcgt ccaccagcat gctgcgccac tctatctccg
      661 ggtagccggc ggctacctcc gcgcacacct cgtcccacag caccgaggtg tactgctgcg
      721 cgttcgactt ggtggcgctg atgaaaggcg cacctggccg aggtccgcgc gagcgtgcaa
      781 gctcgaaggc gtagcgcacc acccgcgcca caccggtccg tgtgaacacg gtcgtctggg
      841 tagcgacctc ctggggcctg cctaaatggc tgcgcccgcc gtgcccgctg tactccccct
      901 cgctgttctc gcgcaccacc acgaaattta tctctttggg gcctttgtcg cggagggggc
      961 cggggatgcc ctcaagcagg cggatggggc gcaggttgat gtactggtcg aagccctggc
     1021 ggatgggcag cagcaggccc cacagcgaga tatggtcggg caccgtggga tagcctaccg
     1081 cgcccaggta tatcgcttcg aatggctcca actgcttgag gccgtcgggg tccatcatgc
     1141 ggccgtgttg caggtagaac tcgcagcccc acgggaagtg gcgcgtgttg aggcggaagc
     1201 tcccggtcag ttcgcccgcc gcctgtagca ccttcagccc ggcggggatg acctcgttgc
     1261 ctatgccgtc gccgggtatc aaagcgatgt cgtactggtt cacgtgttgc tcctgggtga
     1321 cgctcgatgc aggtaaccac cggcgtcggg gcatggatgg gtgtgtgctt ggtaattata
     1381 caatgtaggc aaccctcctg gtggacgggt gctaaggaca cgccctgcta cggctgtgtc
     1441 agagggggat accacggcca gagccgaacc ttgtcatcct gagcgtagcg aaggatcagg
     1501 tgggtaggcg agagtctgca acgtaggaga tgatggcgga tagaggaacc tcggggcctc
     1561 agagggagag tcaagaacgg gcaactgatc cttcgctacg ctcaggatga caaggccact
     1621 ttagttccgg tctccagctt tggcaaagtc gtgaaaccat actgttcggc ctggtctccc
     1681 caccgcccgg cacgatatac tgttgcggac ggttttccgt cccacgccaa ccaccacaac
     1741 caggcatacg aggtagcttg ctatgaccac acccacaccg gcatatcttg acaccacccg
     1801 ccccctgacc gagcgcgtca ccgacctggt ctcgcaactg acgttggagg agaagctcag
     1861 ccagttgcag aactccacgc cgggggtgcc acgcctgggc ataccggctt acaactattg
     1921 gaacgaggcg ctgcatgggg tggcccgcaa cggccgcgcc accatcttcc cgcaggctat
     1981 cggcatggcc gccacctggg acccgcccct catccagcgc gtcgcatccg acattggaga
     2041 cgaggcccgc gccaagtacc acgaggcgct caggcgtaac ggctttacgc ggctctacca
     2101 ggggctgaac ttctggtcgc ccaacgtcaa catctaccgc gacccacgct ggggccgtgg
     2161 acaggagacc tggggcgagg acccctacct caccggccag atgggggccg ccttcgtgcg
     2221 gggcatgcag ggcgaccacc cccgctacct caaggcggcg gcctgcgcca agcactacgc
     2281 cgtgcacagc ggccccgaaa agcagcgcca tggtttcgac gcccacgcga gcctgcgcga
     2341 tatgcacgag acgtacctgc ccgccttcaa ggcgctggtg gacgcggggg tcgagtcggt
     2401 gatgggggcc tacaaccgca cgaatggcga gccgtgctgc gcccactcct acctgattgg
     2461 cgaggtgttg cgcgggcagt ggggcttcca gggccacttc ctctccgact gcggcgctat
     2521 cgacgacatc tacagggggc acggcgcggc ccccgacgca gcatcggcgg cggccctggc
     2581 cctcaagcgg ggctgcgacc tggaatgcgg cggcacctac cggcacctgg gcgaagcgtt
     2641 ggagcgcggc ctggtgacaa tggacgacat cgacagggcg ctgtcgcgag tgctagcaac
     2701 ccgcttcaag ctcggcatgt tcgacccgcc ggagcaggtg ccgtatgcct cgacgcccct
     2761 gagcgtggtg ggcagcgagg cgcaccgggc gctcagctac gaggcggcgg tcaaatcggt
     2821 ggtgctgctg aagaaccgca acaacatctt gccgctcagc ccggacgtgc ggacgatgcg
     2881 cgtgctcggc cccacggcgg tggacgtgag cgtgctgctg ggcagctacc acgggctgaa
     2941 cggcacgctc accacgctgc tcgaaggtat cgtgtcccgg atgcctgagg gggtcggcat
     3001 ggagtaccgc atgggcgtgc agctaatcca ccccaacacg gcctcgcagg agtggttgta
     3061 cagccccagg tcgcagcctg acgtggtgat cgcgtgcatg ggcgtcaccc ccatgctgga
     3121 aggcgaggaa ggcgactcga tagcctccac ggaggagggc gaccgtgcgg ggataagtct
     3181 gccgagcgtg caggccgagt tcgtgcgcaa gctggcgggg gcgggtgcga aagtggtgct
     3241 ggtgctcacg gggggcgggc caatcgcgct gggcgacctg gaggagctgg tggaggcggt
     3301 ggtgttcgtg tggtaccccg gcgaggcggg cgggctggcc gtggccgacg tgctgttcgg
     3361 gcatgtcacc ccctccggca agctgcccct caccttcccg cgctccctgg acgacctgcc
     3421 gcccttcgat gactacggca tgcagggccg cacctaccgc tacgcagaca aagagccgct
     3481 ctaccccttc ggcttcggcc tgagctacac ccggttcgcc tacagcgacc tacagatgga
     3541 gcgagagcaa atcgctgccg gggatgaact ggcggtcagt gtgacgctca ccaatagcgg
     3601 caatgtggat ggtgaggagg tcgtgcaggt ctacgccagc gcgttggacg tgccgggcgc
     3661 gccaatcagc agcctggtgg ccttccggcg ggtggcgttg cgggcgggcg agcgcaagtc
     3721 gctggccttc gaggtgcctg cggagcggct ggcgttggtg gatgaggagg ggattgcgcg
     3781 ggtggtgccg gggcgcatga ggctcacggt gggtgggtgc tcgccgggag aacgggggct
     3841 aaacctgggt gccgcgcggc ccgcgagcgg cgagttcctc attctgggac acatatagct
     3901 ggctcttgaa gtctacggtg ttatcaaata caatatggcc cagatacaat atggcccaga
     3961 gagcctcacg atagacacgc ggagttacta tatagctccg cgtgttccac ttatgacatc
     4021 tacgcttaac actcagacag cgctacttat gtggtgaaca gtcttgttgc gaatagcccc
     4081 ttgcggctct tagccctggg tgaaagggcg acgtgggggt ctcacagagg gggagcaccg
     4141 atgaacgaag cacaagccga tagcagcgtg caaaccgacg gtgggcatgc atacgaagca
     4201 ggtacgagcg atgaggccag gcaagaggct ctcgcgcccg tcacacagca acctgtaagc
     4261 cccgctgacg aggtcgcgac acgggcaatc gatggcagcg cggcagaggc cagggggtcg
     4321 ggtaagccca ggtccggcat agctctatgt ctgtcgggcg ggggctaccg ggcgatgctc
     4381 ttccacctgg gcgcgttatg gcggctcaac gagcttggct
//
LOCUS       DASRWZ010000250         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1184774, whole genome shotgun sequence.
ACCESSION   DASRWZ010000250 DASRWZ010000000
VERSION     DASRWZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184774"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFH60_03905"
     CDS             <1..825
                     /locus_tag="VFH60_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312956.1"
                     /translation="ANTSSGSVLFKETGKRLGGRFLEYWQKNGGLPQQGYPISDEFTE
                     KSDLNGQEYRVQYFERAVFEYHPEQKPPYDVLLSHLGTFRWRAKYETQANCGSPILPG
                     LWMGQLTWGFTMSGSSVAGNGGVSGPVRLNVACDGTFSGTVRIENYRTRIGSGNFAIL
                     TCTQSEVVVANIAGRVERVADGKRLNILGGVFQQGIYTCVNPLAPAQPTRLAGNTVPP
                     TMVEIEQESRTKISGSQWVPDPSYVSLIEQFRSAYRDVQIVYTGNWDLERQEINTP"
     gene            complement(949..>2649)
                     /locus_tag="VFH60_03910"
     CDS             complement(949..>2649)
                     /locus_tag="VFH60_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312957.1"
                     /translation="VYSETRRHAPGLLAARLWLSLDIVYSWSTFYKQDVPSIGLALLG
                     CALLSLWHSRKSAGANPKLALERSEGSKIQNLKSDLLFYASILPMSLAFWLKQSTVAQ
                     FAAGGLFLLLVSWRLGVRWGVWAAASIVVPFLGFLALSKGGLAEHLLAFNAFGRSTAR
                     LWQRLDALWVNFTPLVICGAVFLVGALAWAARKKGLPPLSALFLLFSIPVALLALLHP
                     SGNYNHLLNLLAPLCLAVGLMLGHASNWLGGSGEHRRLAWTGAGLLAGVLVAVLAQAV
                     LTYNKPFYEWYSPLALPLEERAERMRQLDKVVQDTPGSIFSEDQWLLLKNGKQVLYDD
                     PVAMAALSRSGAWDESVLLEDLARRKFALVILQYDLTGELYNPRWSDRGLATVQASYT
                     RRFRDVYFTYSPTSPPQEPNTATNCNMAGGPTMQGFTFGITTANRGDNLPLSLYWREP
                     GGEAERQPSLKVFARLIDGTGQPRWQADWQPGELAGKPWAGGGWPPDDTVRDDLWVPI
                     TGDLPYGRYRLQTGFYSQGADGKIAPATPTCSGDAGVDPDGTVILADLTVVERWSERP
                     "
BASE COUNT          490 a          794 c          943 g          422 t
ORIGIN      
        1 gccaacacat cgagcgggtc ggtgctgttc aaggagacgg gcaagcggct cggcgggcgg
       61 ttcctggagt actggcagaa gaacggcggc ctcccgcagc agggctaccc gataagcgac
      121 gagttcacgg agaagagcga cctgaacggc caggagtacc gggtgcagta cttcgagcgt
      181 gcggtgttcg agtaccaccc tgagcagaag cccccttacg acgtgctgct gtcgcacctg
      241 ggcacttttc ggtggcgggc caagtacgag acgcaagcca actgcggctc gcccatcctg
      301 cccggcctgt ggatggggca gttgacctgg ggctttacga tgagcggttc gtcggtcgcg
      361 gggaacggcg gggtatcggg gcctgtgcgc ctgaacgtgg cctgcgacgg cactttttcg
      421 ggcacggtgc gcatcgagaa ctatcgcacg cgtatcggca gcggcaactt tgctatcctg
      481 acttgcacgc agtcggaagt ggttgtagcc aacattgcgg ggcgggtgga gcgggtggcc
      541 gacggcaagc ggctcaacat actcggcggg gtcttccagc agggcatcta tacgtgcgtg
      601 aaccccctgg ccccggcgca gcccacccgg ttggcgggta acacggtgcc cccgacgatg
      661 gtcgagatag aacaggagag tcgcaccaaa atcagcggca gccagtgggt gcccgacccc
      721 tcgtacgtga gcctgataga gcagttccgc agcgcctacc gggacgtaca gatcgtctac
      781 accggcaact gggacctcga gcggcaggag atcaacacgc cgtaggagcg agtgccgagt
      841 gccgagtgaa gagacgaaaa cgcaaagacg caaagaacgc aaaggacgcg caaagtttaa
      901 gatggttgga attggtgccc ctcggtactc ggcactcggt actcgctcct aaggccgttc
      961 gctccagcgt tccactaccg ttaggtcggc caggatgacg gtgccgtcgg ggtctacgcc
     1021 cgcgtcgcct gagcaggtag gggtggcggg ggcgattttg ccgtccgcgc cctggctgta
     1081 gaagccggtt tgcaggcggt agcggccgta tgggaggtcg ccggtgatgg gcacccagag
     1141 gtcgtcgcgc accgtgtcgt cggggggcca gccgcccccg gcccagggct tacccgcaag
     1201 ctcgcccggc tgccagtcgg cctgccacct gggctgcccc gtgccgtcga tgaggcgggc
     1261 gaacaccttg aggctaggct ggcgctcggc ctcgccgcct ggctcgcgcc agtagaggct
     1321 caatggcagg ttgtcgccgc ggttggcggt ggtgatgccg aaagtaaagc cctgcatggt
     1381 ggggccgccc gccatgttgc aattcgtggc ggtgtttggc tcctggggag gactggttgg
     1441 cgagtaggtg aaatagacgt cgcggaagcg gcgggtgtag ctcgcctgga cggtcgccag
     1501 gccccggtcg gaccagcgcg ggttgtacaa ctcgccggtg aggtcgtatt gcaggatgac
     1561 gagagcgaat ttgcggcgcg ccaggtcttc cagcagcacc gactcgtccc aggccccgct
     1621 acgagaaagg gcggccatag ctaccgggtc gtcgtagagc acctgcttgc cgttcttcag
     1681 cagcagccac tggtcctcgg agaagatgga gccgggggtg tcctgcacca ccttgtctag
     1741 ctgccgcatc cgttcggccc gctcctccaa cggcagggcc agggggctgt accactcgta
     1801 gaacggcttg ttgtaggtga ggacggcctg ggcgagcacc gccaccagca cgcccgccag
     1861 cagcccggca cctgtccaag cgagccgcct gtgctccccg ctgcccccca gccaattcga
     1921 ggcgtgtccc agcatgaggc ccacggccag gcagaggggt gccagcaggt taagcaggtg
     1981 gttgtagttg ccgcttgggt ggagcaaggc gagtagcgcg accgggatag agaacagcag
     2041 gaacaacgcg ctcagcggcg gcaggccctt tttgcgtgcg gcccaggcga gcgcgcccac
     2101 caggaacacc gcaccgcaaa tcaccagcgg ggtaaagttc acccagagcg cgtcgagtcg
     2161 ctgccacagg cgagcggttg agcgtccgaa cgcgttgaag gccagcaggt gctcggccag
     2221 gccgcccttc gagagggcga ggaagcccag gaagggcacg acaatagaag ccgccgccca
     2281 cacgccccac cgcaccccca gccgccagct gacgagcagc aggaacaatc cccctgccgc
     2341 gaactgcgcc accgtcgatt gcttcagcca gaacgcgagg gacattggta ggatggaggc
     2401 gtagaagagg aggtcggatt ttagattttg gattttggac ccttcgcttc gctcaagggc
     2461 aagctttgga tttgcgccgg cggatttgcg ggagtgccag agggagagga gggcgcagcc
     2521 gaggagggcg agaccgatgg aggggacgtc ttgcttgtag aaggtgctcc agctgtagac
     2581 gatgtcgagg gagagccaca ggcgggcggc gaggaggccg ggggcatgcc gcctggtctc
     2641 gctgtagac
//
LOCUS       DASRWZ010000251         4806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_156957, whole genome shotgun sequence.
ACCESSION   DASRWZ010000251 DASRWZ010000000
VERSION     DASRWZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156957"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1724
                     /locus_tag="VFH60_03915"
     CDS             24..1724
                     /locus_tag="VFH60_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6312958.1"
                     /translation="MKRVTVTLLSFIVAFVVAFSPSMAQTRSSAPDTGKGRVDLSAPA
                     VPGQFVVKFRDGISDSAREVAITSLGGRFFDRIEAQDVDAVEFPALAKGSSLEATEAI
                     LNALKQNPNVEYVEPNYIYSTSFTPNDPGRTQQYAWNNIQAYSAWDVTQGSSSVVIAI
                     VDTGIQRNHPDLDAKIVAGYDYVQNDTAPDDGNGHGTHVAGTSAAETNNGIGGAGTCP
                     NCRLMPVRVLDNSGSGTLVNVANGINFAANNGAKSINLSLGGSGSTTLQNAVDNAWNR
                     GAFLACAAGNSNTSSTASAYPAAYPNCFAIASTTSSDARSSFSNYGSWVEVAAPGSSI
                     YSTWLNSGYNTISGTSMATPHVAGLAGLLASQGLTNSQIRTRICNTADRISGTGSLWT
                     CGRINAYRAVTGSGGTPTPTPTPPPGGTERIVNGGFESGTSPWVQSSANGYQLIDPTR
                     PRTGTYSAWLCGYNNCNDNIYQTVTVPTNGTLTYWWYMTTQETSHPWDYLRVRLYNTS
                     GTLLTTLRTWNDGSGAGVWRQDSISLAGYAGQTVRVHFGATTDGSLTTSFFVDDVSLR
                     "
     gene            1891..3324
                     /locus_tag="VFH60_03920"
     CDS             1891..3324
                     /locus_tag="VFH60_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET6312959.1"
                     /translation="MWIGIVSIILLVLATAFFVAAEFALVSVRRTRIDQLVSEGNALA
                     KDVRRALDRLNTYLAAAQVGITMATLGLGALGEPVLAQVLVPPLEAILPHEWVEQFIS
                     IHGIAFVIALLLVTITELIIGETVPKIWAIQRAEAMSLVVIKPMNLMLLIFKPLIWLI
                     NTLSNAILRMIGLPQDKGHETVYSVEELEMLVVSSRQAGVLDKQEEVILRRVFDFGEL
                     TARQVMRPRTEVVGVPVDDTLSGVISTMLEHKHSRFPVFEGDMDNIVGVLFVLDVFAY
                     LAQTTDSLEAAGVGADGVVRAVVAGVSPSPGLAANQFNVRSLMRPIDAVPETLDVALL
                     LARMQQSGTQMVVVVDEYGGTAGIVTLEDVVEEIVGEVRDEFEPESTTSGIVVTPEGT
                     LVDGLVAIDDVSEALGLKIESESDTVGGYVFEMLGRKPELGDTVSVDGFTLRVEELDG
                     LRIARVRVLSGRNGQGQQVPQEQTDEE"
     gene            3437..4666
                     /locus_tag="VFH60_03925"
     CDS             3437..4666
                     /locus_tag="VFH60_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6312960.1"
                     /translation="MNRPVRGEGRPGERPTSVSRVARGGRPAAGVRRPGRPTMGESSP
                     GTSRPGKAERRSGVGRPATNEGQPGRPAPTGSRAGHRATTANRPSASRPPVRSEGRRH
                     ARPQAKAAAQDGERLQKILARAGVASRRAAEEMIAAGRVTVNGTLVTEMGARADPAKD
                     TIKVDGTALNVPALETQAQEMVYIAMHKPKDVVSTAKDTHGRPTVLDLLRGTGGEERG
                     VRDQGPGIRGQGPGGSSSQRGRPTPNTQQPSPDVKQRVYPVGRLDADSTGLLLLTNDG
                     DLTFRLTHPKYGVEKEYRVLVRGKPGEAVLNRLREGVEIEGEITAPAKVEELGRRDND
                     TWLRITIREGRKRQVRLMAAAVGYHVVELQRVRFGPILLGNLIPGKWRYLAQHEIHAL
                     RKAVKLPISEHAKRPAR"
BASE COUNT         1006 a         1389 c         1615 g          796 t
ORIGIN      
        1 ttagtcgcac aaggagaaag atcatgaaac gggttacggt gacactgctg tcgtttattg
       61 tggcgtttgt agtggccttt tctccatcta tggcgcagac caggagttcc gcgcctgata
      121 cggggaaagg gcgagtagac ctgagcgcgc ccgccgtccc agggcaattc gtggtgaaat
      181 tcagggacgg catttcggac agtgctcgtg aggtggcaat tacttcactc ggcgggcgct
      241 tcttcgaccg catagaggcc caggacgttg acgcggtgga gttcccggcg ctggcgaagg
      301 gcagcagcct ggaagccacc gaagcgatac taaacgccct caagcagaac ccgaacgtcg
      361 agtacgtgga accgaactat atctactcca ccagcttcac cccgaacgac cctggccgca
      421 ctcagcagta tgcgtggaac aatatccagg cttattccgc gtgggacgtt acccagggca
      481 gctccagcgt ggtcatcgct atagtggata cggggataca gcgcaaccac cccgacctcg
      541 acgctaagat cgtcgcgggc tacgattacg tgcagaacga caccgcgccc gacgacggca
      601 acgggcacgg cacccacgtc gcgggcacga gcgcagccga gacgaacaac ggcatcggcg
      661 gggcgggcac ctgccccaat tgcaggctga tgccggtgcg cgtgctcgac aacagcggta
      721 gcggtaccct cgtgaacgta gccaacggta tcaacttcgc cgccaacaac ggcgcgaaat
      781 ctatcaactt gagcctgggc gggagcggct cgaccaccct gcaaaacgcg gtggacaacg
      841 cctggaaccg gggcgcattc ctggcgtgcg ccgcaggcaa cagcaacacc agcagcaccg
      901 ccagcgcata ccctgccgca taccccaact gcttcgccat cgcgtccacg accagcagcg
      961 atgcgcgctc gtccttctcc aactacggct cgtgggttga ggtcgcggca cccggctcct
     1021 cgatctattc gacctggctc aacagcggct acaacacgat aagcggcacc tcgatggcga
     1081 ccccgcacgt cgcgggcctg gcgggcttgc tggcttccca gggcctgacc aactcgcaga
     1141 tcaggacgcg catatgcaat accgccgacc gcataagcgg cacgggcagc ctgtggacct
     1201 gcgggcgcat caacgcttac cgcgcggtga ccggcagtgg tggcacgccc acacccacgc
     1261 ctacccctcc accgggcggt accgagagga tagtgaacgg cggcttcgag agcggcacaa
     1321 gcccctgggt gcagtcttcc gctaacggct accaactgat agaccccacg cgtccgcgca
     1381 caggcacgta cagcgcgtgg ttgtgcggct acaacaattg caacgataac atctaccaga
     1441 cggtgacggt gcccaccaac ggcacgctca cctactggtg gtatatgacc acccaggaga
     1501 cgagccaccc gtgggactac ctacgcgtgc ggctctacaa cacaagcggc acgctcctta
     1561 ctactctacg cacctggaac gacgggagcg gcgcgggagt atggaggcag gatagcatca
     1621 gcctggcggg ttacgcgggc cagaccgtgc gcgtccattt cggcgcgacc accgacggct
     1681 ccctgacgac cagcttcttc gtggacgacg ttagcctgag gtagaattaa gaacaactaa
     1741 caaagtctga ttgtctgcta cgtaggggcg gttcgcgaac cgcccctact tttgaaaggc
     1801 ttggccaggc cgctccgcgc atgaccgagc ctatttgttg ttttatagga tgcttttgat
     1861 ggcggtctga aaactgctat tatatctgtt atgtggatag gtatcgtctc gataatcttg
     1921 ttggtgctgg ctacggcatt cttcgtggct gccgagttcg cgctggtgag cgtccggcgc
     1981 acgcgcattg atcagctagt cagtgaaggc aacgccctgg caaaagatgt caggcgcgcg
     2041 cttgaccgcc tgaacactta cctggcggcg gcgcaagtgg gcatcactat ggcgacgctg
     2101 gggcttggcg cattgggcga gccggtgctg gcgcaggtgc tcgtccctcc tctcgaagct
     2161 attctgcctc atgagtgggt cgagcagttc atctccattc acggaatcgc tttcgttatc
     2221 gccttgctcc tggtgactat caccgagctt atcataggtg agaccgtgcc gaagatatgg
     2281 gctatacagc gcgcggaggc tatgtctttg gtggttatca agccgatgaa cttgatgctg
     2341 ctcatcttca agcccctcat ctggcttatc aacacgctca gcaacgccat acttcgcatg
     2401 attggattgc cccaggacaa agggcatgag accgtctact cggtggaaga gcttgagatg
     2461 cttgtggtat cgagccgcca ggcgggggtg ctcgacaagc aggaagaagt gatcctgcgc
     2521 agggtgttcg atttcggaga gcttaccgcc cgccaggtga tgcgcccccg cacggaggta
     2581 gtcggggtgc cggttgacga cacgctatcc ggcgtcatct cgaccatgtt ggagcacaag
     2641 cacagtcgct tccccgtctt cgagggcgac atggacaata tcgtgggcgt gctcttcgtc
     2701 ctggacgttt tcgcgtacct ggcccagacg acagatagcc tggaagccgc cggggtgggg
     2761 gcggacggcg tggtgcgcgc ggtggtggcg ggggtgtcgc catcgcccgg cctggctgcg
     2821 aaccagttca acgtgcgctc tctcatgcgc ccgatagatg ccgtgcctga gacgctggac
     2881 gtggcgctct tgctggcgcg catgcagcag agcggcacgc agatggttgt ggtagtagac
     2941 gagtacggcg gcacggcagg catagtcacc ctggaggacg tggtagagga gatagtgggc
     3001 gaggtgcgcg atgagttcga gccggagagc actacatcag gcatagtggt cactcccgaa
     3061 ggcacgctgg ttgacgggct ggtagcgata gacgacgtaa gcgaggcgct ggggctgaag
     3121 atagagagcg agtcggacac ggtaggcggc tacgtcttcg agatgttggg ccgcaagccg
     3181 gagttgggcg acacggtgag cgtagatggc ttcaccctgc gcgtagagga gcttgacggc
     3241 ctgagaatag ccagggtgcg cgtgctatca ggcaggaacg ggcaggggca acaggtcccg
     3301 caggagcaga cagatgagga atgaaggccg cccaggcagg cgcggggcag cgcggagcga
     3361 gggtcgccca ggcaggcgaa caagaagcga cggccctccc gccagcagac cgggcgcaaa
     3421 caggggtcgc cccggcgtga ataggccggt gaggggggaa ggcaggccgg gcgagcggcc
     3481 aacgagtgta agccgcgtcg ccaggggtgg caggccggcg gctggtgtac gtcgccccgg
     3541 caggcccacg atgggtgaga gcagtccagg tacgagcagg ccagggaagg cagagaggcg
     3601 ttcgggcgtg ggtagaccgg ccacgaatga gggccaaccc ggcaggccag caccgacagg
     3661 gagcagggcc gggcatcgag caaccactgc caatcgccca agcgcgagca gaccgccggt
     3721 aaggagtgag ggtcgccgtc atgcgaggcc gcaggcgaaa gcggcagcgc aggacggcga
     3781 gcgcctgcaa aagatactcg ccagggcggg tgtggcttcg cgccgggctg cggaggagat
     3841 gatagcggcg ggccgcgtga ccgtcaacgg cactcttgtg acggagatgg gagcgcgcgc
     3901 cgaccccgcg aaagacacca taaaggtaga cggcacggcg ctcaacgtgc ccgcactcga
     3961 aacgcaggcc caggagatgg tctatatagc catgcacaag ccgaaggacg tggtatccac
     4021 cgctaaggac acgcatggcc ggcctacggt gttggacctt ctgcggggga cggggggcga
     4081 ggagcgaggg gtcagggacc agggaccagg gatcaggggc cagggaccag gagggagcag
     4141 cagccaacga ggtcgcccaa cacccaacac ccaacagcca tcacccgatg tcaagcagag
     4201 ggtttacccg gtggggaggc tggatgcgga cagcacgggg ctgctcctgc tgactaacga
     4261 tggcgatttg acctttcgcc ttacgcaccc caagtatggc gtcgagaagg agtaccgggt
     4321 gctggtgagg ggcaaacctg gggaggcggt gctgaacagg ttgcgcgagg gggtagagat
     4381 cgagggtgag ataacggccc ccgcgaaggt tgaggagcta ggcaggcgcg acaacgatac
     4441 gtggctgcgc atcacgatac gcgaagggcg caagcggcag gtacggctaa tggcggcggc
     4501 ggtggggtat cacgtagtgg agctacagcg cgtgcggttc ggccccatac tgctgggcaa
     4561 tctcatcccc ggcaaatggc gatacctggc tcagcacgag atacatgcgc tgcgtaaggc
     4621 ggtcaagctg cccatctcgg agcatgcgaa gcgcccggca agataggcta taatgtcctg
     4681 aactgaattc ttatcaagcg aaccccatca ggtaagtgtt tagataggga taaaggaatg
     4741 tttcagtagg ggcgacccgg tgggtcgccc tggttggggt agcaagtgag catggtacgc
     4801 catccc
//
LOCUS       DASRWZ010000252         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1116467, whole genome shotgun sequence.
ACCESSION   DASRWZ010000252 DASRWZ010000000
VERSION     DASRWZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116467"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFH60_03930"
     CDS             <1..583
                     /locus_tag="VFH60_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6312961.1"
                     /translation="LVLIQSKGTDENGNEDQSLGSGVVVSDQGDILTSLHVVTASTEI
                     RLTFADGTESLAQVTVAQPETDIAVLHALQPPAEIFPAVLGSPAAMSVGDEAFVVGNP
                     FGLYSSMSAGVISGFGRSFKPVESEYRLNDLIQIDAAVNPGNSGGPLLNRYGEVIGIV
                     TGLVNPTEQNFFVGVGFAVPINLAAPAAGAPPW"
     gene            690..1712
                     /locus_tag="VFH60_03935"
     CDS             690..1712
                     /locus_tag="VFH60_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6312962.1"
                     /translation="MDQFKRPEDRLPMERVLYEVKKVIVGQDHLLEQMIVALLARGHM
                     LVEGVPGLAKTMAIKTLAEAIGGEFKRIQFTPDLVPADLIGTRIYNQKTGEFNTVLGP
                     VFTNLLLADEINRAPAKVQSALLEVMQERQVTIGLETFQVPNPFLVLATQNPIETEGT
                     YPLPEAQVDRFMFKVLIDYPSPTEEFVIVERMTGVLQAVQKVLTTAHLLELQQAADRV
                     YVDPALIQYAVHIVGATRAPKDVGLRELQPYIMYGASPRASINVIRAAKALAFVRGRA
                     YALPQDVLDMALDVLRHRLVLSYEALSDNVTGDDLLNRILDRLPAPVVPLHEHTNNHA
                     SVRTSA"
     gene            1669..2580
                     /locus_tag="VFH60_03940"
     CDS             1669..2580
                     /locus_tag="VFH60_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6312963.1"
                     /translation="MSTPTTMQASAQAPERILQRLDWQVIRRLDGLLQGDYRTLFYGS
                     GVDFADLREYQPEDDVRYIDWNVTARMDSPYVRQYTEDREITAWFLLDLSPSMDFGSM
                     RSLKRSALIDLVATLTRLLTRHGNRVGAMFYGSRVERTIPAHGGQVQVLRLINDLQKQ
                     PQLPSAPFTDLGPLLEAGLHALKRRSLVFVISDFISIPGWERRMSLLNQRHEVIAIRL
                     WDPREVELPDIGPVIMEDAETGEHLYVDTHDRNFRKRFKEAARQREAALGEAFKHAGV
                     DVLSLSTEEDLVLALVRFAGLRRQRKR"
BASE COUNT          537 a          836 c          892 g          466 t
ORIGIN      
        1 gctcgtgctc atccagtcta aaggcacgga tgaaaacggc aatgaggacc agtcccttgg
       61 cagcggcgtg gtcgtcagcg accagggcga catccttacc agcttgcacg tcgtcaccgc
      121 gtccaccgag atccggctga cgttcgcgga cggcaccgag tcgctcgccc aggtgacggt
      181 ggcgcagccg gagaccgaca tcgcggtatt gcacgcgctc caacccccgg cggagatatt
      241 cccggcggtg ctcggcagtc cggccgctat gagcgttggg gacgaagcgt ttgtggtggg
      301 caatcccttc gggctgtata gctcgatgag cgctggggtc atctccggct tcggccgctc
      361 gttcaagccc gtggagagcg agtacaggct gaacgacttg atccagatag acgcagcggt
      421 caaccccggc aattcgggcg ggccgctcct gaaccgctac ggagaggtga taggcatagt
      481 cacgggcctg gtgaatccga cggagcagaa cttctttgtg ggcgtgggct tcgccgtacc
      541 cataaacctg gccgcaccgg ccgcgggagc gccaccctgg taaagggtga ggaacgggac
      601 cagggtggcg aacgcaagca atccagcggc gcacctgggg cacagggtgt ggctgcgcag
      661 agggcataga gacttagagg agatggagaa tggaccagtt caagaggccg gaagataggc
      721 tgcctatgga gcgcgtgctg tacgaagtga agaaagtgat cgtcggccag gaccatctac
      781 tggagcaaat gatcgtggcg ctgctggcgc gcgggcacat gctggtcgag ggcgtgccgg
      841 ggcttgccaa gacgatggcc atcaagacgc tagccgaggc cattggcggt gagttcaagc
      901 gcatccagtt cacgcccgac ctcgtgcctg ccgacctgat tggcacgcgc atctacaacc
      961 agaagacggg cgagttcaac acggtgctgg ggccggtctt caccaatctc ctgctggcgg
     1021 acgaaatcaa ccgggcacca gccaaggtgc aaagcgccct gctcgaagtg atgcaggagc
     1081 ggcaggtgac gattgggctt gagaccttcc aggtgcccaa ccccttcctc gtgctcgcca
     1141 ctcagaaccc catcgagact gaaggcactt accccctgcc ggaggcccag gtagaccgct
     1201 tcatgttcaa ggtgctgatc gattacccca gcccgacgga agagttcgtc atcgtggagc
     1261 gcatgacggg ggtgctgcaa gcggtgcaga aggtgctgac caccgcgcat ctgctggaat
     1321 tgcaacaggc cgccgacagg gtgtacgtgg acccggccct tatccagtac gccgtgcata
     1381 tcgtgggcgc cacccgcgct ccgaaagacg ttggactgcg ggagctgcag ccctatatca
     1441 tgtacggggc gagtcctcgt gcctcgatca acgtgatacg cgccgccaaa gccctggcct
     1501 tcgtacgggg gcgggcctac gcgctcccgc aggacgtgct ggacatggcc ctcgacgtat
     1561 tgcggcaccg cctggtgctg tcttacgagg cgctttccga caacgtgacc ggcgacgacc
     1621 tgttgaacag gatcctcgac cgcctgcccg ccccggtggt accgttacat gagcacacca
     1681 acaaccatgc aagcgtccgc acaagcgcct gagcgcatcc tgcaacgcct cgactggcag
     1741 gtcatccgcc gtctggatgg cctgttgcaa ggcgattacc ggaccctgtt ctacggctcc
     1801 ggggtcgatt ttgccgacct gcgcgagtac cagcccgaag acgacgtgcg ctacatcgac
     1861 tggaacgtca cggcgcgcat ggactccccc tacgttcgcc agtatacgga ggaccgggag
     1921 atcaccgcgt ggttcctgct cgacctgagt ccctcgatgg acttcggcag catgcgtagc
     1981 ctgaagcgtt cggcgctgat agacctcgtc gccacgctga cgcgcctgct cacccggcac
     2041 ggcaaccgcg ttggggccat gttctacggc agccgcgtcg agcgcaccat cccggcacat
     2101 ggcgggcagg tgcaggtgct acgcctgata aatgacttgc aaaagcagcc gcagctaccg
     2161 agcgccccct tcacggacct cggcccgctg ctggaagctg gattgcacgc gctcaagcgg
     2221 cggtcgctgg tgttcgtcat ctcggacttc atcagcatcc ccggctggga gcggcgcatg
     2281 agcttgctca accagcggca cgaggttatc gctatccggc tgtgggaccc gcgagaagtc
     2341 gaattgcccg atatagggcc ggtcatcatg gaagacgccg agacgggcga gcacctctac
     2401 gtggacacgc acgaccgcaa cttccgcaag cgcttcaaag aggccgcccg ccagcgggaa
     2461 gcagccctgg gcgaggcttt caagcacgcg ggggtagacg tgctgtcgct ctcgacggaa
     2521 gaggatctgg tgctcgcgct ggtgcgtttc gcggggctgc ggcgacagcg caagagatga
     2581 cggtggattg cagtacaaaa gccccccttg tcatcctgag cgtagcgaag gatcaggtgg
     2641 gtaggcgaga gtcttcaacg cggtggatga tggcggctag aggaacattg gtgcaatagt
     2701 aggaagagtc aaacacgggc ggctgatcct t
//
LOCUS       DASRWZ010000253         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1185270, whole genome shotgun sequence.
ACCESSION   DASRWZ010000253 DASRWZ010000000
VERSION     DASRWZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185270"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VFH60_03945"
     CDS             complement(<1..952)
                     /locus_tag="VFH60_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6312964.1"
                     /translation="MPALLLVLVLLLQACGGPTAAPTSTVVAPADTATSAPAATTDPT
                     AAATTAPTTEATAMSGETPESDLSGTVTFWSSYNTVSPEMETLTGEIIPAFQKLYPNV
                     TVKAQALPGDELRQKLLTSIAGGETPDVVRVDIIWVPEFAELGALAKLDEEMEDFEDY
                     KEQVYPGPLSTNFHKGNYYGLPLDTNTKVAFYNNDVLAEAGMSAPPKDWAEFTSFCEK
                     VKALNKPDTYCYAESGTGPWSILPWIWAGGGSITDPEYTKATGYVNSPQTVAALTTLR
                     DLLKNELISPSIMGGGMATSDALGKGHAALIIDGPWMPPIF"
     gene            complement(1176..2396)
                     /locus_tag="VFH60_03950"
     CDS             complement(1176..2396)
                     /locus_tag="VFH60_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET6312965.1"
                     /translation="MVLRLRSGNRELIREINKSLVLSLVRDHEPISRTEISQTVNLSP
                     ATVSGFTGELIEQGLIYEHEAGTSTGGRRPILLALNRQGGFVIGIKLAETHIVAALTD
                     LGANVIDQRAAPLSDDDRQPEAVVAVLADTVNKLRSLHPERNIFGVGLGMAGAVDRRL
                     GICHFSPFLNWHDVPLRRMLEQRLELPVVLENDVNTLTMSEKWFGAGVGVNDFLVITL
                     GRGIGMGMVLNGQIYRGGCGGGGEFGHITVVPDGPQCECGKRGCLEALVSDPAILRRM
                     RSAIGRDLTMSEAAVLARQGDSTAQGIFNAAARTLGMAIADLVNIFNPPLLIVGGEGA
                     RAFDLLLEPLQEALQANCFNGFFDDMRLVVEPWGDDAWARGAASLMLEELFRPTLYHD
                     DKARASLVLPISSL"
BASE COUNT          467 a          774 c          927 g          523 t
ORIGIN      
        1 cgaagatggg gggcatccag gggccatcga tgatcagagc ggcgtggccc ttaccgagcg
       61 cgtcgctggt agccatgccg ccacccatga tggagggcga gataagctcg ttcttcagca
      121 ggtcgcgcaa ggtggtgagg gccgccactg tctgcgggct gttcacgtag ccggtggcct
      181 tggtgtactc agggtcggtg atgctgccgc cgcccgccca aatccagggc aggatgctcc
      241 aggggccggt gccgctttcc gcgtagcagt aggtgtcggg cttgttcagg gccttgacct
      301 tctcgcagaa ggaggtgaat tccgcccagt ccttgggggg tgcgctcata ccggcctcgg
      361 cgagcacgtc gttattgtag aaggcgacct tggtgttcgt gtcgaggggc aggccgtagt
      421 agttgccctt gtggaagttg gtggagaggg ggccggggta gacctgttcc ttgtagtcct
      481 cgaagtcttc catctcctcg tccagcttgg caagcgcgcc cagctcggcg aactccggca
      541 cccagatgat gtctacgcgc accacgtcgg gggtctcgcc gcccgcgatg gaggtcagca
      601 gcttctgacg tagctcgtcg ccggggaggg cctgggcctt taccgtgacg ttcgggtaca
      661 gcttctggaa ggcggggatg atctcgccgg tgagcgtctc catctcaggg gagacggtgt
      721 tgtaggaact ccagaaggtc accgtgccgc tcaggtcgct ttcaggcgtc tcgcccgaca
      781 tggcggtagc ctcggtcgtg ggcgcggtcg tggctgcggc ggtcgggtcg gtcgtggcgg
      841 ccggggcgct ggtggcggtg tcggcgggtg ctacgactgt cgaggtcggc gcggcggtgg
      901 ggcctccaca ggcctgcaac aataggacaa ggaccagcaa caacgctggc ataaatctac
      961 ggatcttcat tgtatcccct ttctgctcct ctgggttggt actctcggcg tggggtgacg
     1021 aaatgctgga acgtacccgt tgcttggctg atcaggccaa atgctggcct tatgcacacc
     1081 tccatcaagt taattaattg aactaagtaa gtttttgcga aatttgagac cctagctata
     1141 tgggtggttt gtgattgcct gggacgtgaa gggcgctaga gcgagctaat aggaagcaca
     1201 agcgaggcgc gcgccttgtc gtcgtggtac agggtggggc ggaatagctc ttccagcatc
     1261 aggctggccg cgccccgcgc ccaggcgtcg tcgccccacg gctcgaccac gaggcgcatg
     1321 tcgtcgaaga agccgttgaa gcagttggcc tggagggctt cctgcagagg ctcaagcagc
     1381 aggtcgaagg cccgcgcgcc ctcgccgccg acgatgagca ggggcgggtt gaagatgttc
     1441 accaggtcgg caatcgccat gcccagcgtg cgggccgccg cgttgaagat gccctgggcc
     1501 gtggagtcgc cctgccgcgc aagcacggcc gcctcgctca tggtgaggtc gcgccctatt
     1561 gccgagcgca tgcggcgcag gatggcgggg tcggatacca gggcttccag gcacccgcgc
     1621 ttgccgcatt cgcactgcgg gccgtcgggc accacggtaa tgtggccgaa ctcgccaccc
     1681 ccgccgcagc cgccccggta gatctggccg ttgagcacca tgcccatgcc gatgccgcgc
     1741 cccagcgtga ttaccaggaa gtcgttgacg cctacgcccg cgccgaacca cttctccgac
     1801 atggtgaggg tgtttacgtc gttttcgagt acgacgggca gctcgaggcg ctgctctagc
     1861 atgcggcgca ggggcacgtc gtgccagttg agaaacgggg agaagtggca gatgccgagg
     1921 cgacggtcca cggcccccgc catgcccagc cccacgccga agatgttgcg ttcggggtgg
     1981 aggctgcgca gcttgttcac ggtgtccgcg agcacggcga ccacggcttc gggctggcgg
     2041 tcgtcgtcgc tgaggggcgc ggccctctgg tcgatgacgt tggcccccag gtcggtgagg
     2101 gcggccacga tgtgggtctc tgcgagcttg atgccgatta cgaagccgcc ctggcggttg
     2161 agggcgagga ggatgggccg gcggccgccg gtggaggtac cggcctcgtg ttcgtaaatg
     2221 aggccctgct cgatcagctc gcccgtgaag ccggagacgg tggcggggct aaggttcacg
     2281 gtttgcgaga tctcggtgcg cgaaatcggc tcgtgatcgc gcacgaggct caatacgaga
     2341 gacttgttga tctctctaat cagttcgcgg ttgccggagc gcagtcttag caccatgcga
     2401 ataccactcc caatataagg cacgtaaatt cagcaattga aataagttgg tggtaggata
     2461 acccatcttg cagctgctgt caataggccg gggcctaaag cagagcaacg ggggctgttt
     2521 tgcctcttga cagagcagtc aacatccatt aaacttcgct caccaaagaa agtcaaaata
     2581 ttgatgcgtg agggtcgcgc aaccacgaag taggccggcg atgtcccgta caggcaattg
     2641 atgcgttgca ccgcaacgca tcccctatgt gtgtaaagcg tggctgcgga g
//
LOCUS       DASRWZ010000254         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_88991,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000254 DASRWZ010000000
VERSION     DASRWZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88991"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFH60_03955"
     CDS             <1..691
                     /locus_tag="VFH60_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6312966.1"
                     /translation="LPGVYTFTGLIVGRVYAIEDPDGLTIIDAGLPVAPSRIEAQLRA
                     AGHEPGDVKRILVTHAHPDHVGGLPGLKKLTGAEVICSEPERPVVEGKVAIAMMSPYK
                     EMQESRRFKPPPTMLEATPVDRTVGEGDVLDEVLGGLQVLITPGHALGHITFWQPERR
                     IAFCGDVMMHLAGLTLPFGPFTPDMDENRRSIRRIACLEPEAVLFGHGQPLLKDTARR
                     VQAFSDRVNRP"
     gene            941..1279
                     /locus_tag="VFH60_03960"
     CDS             941..1279
                     /locus_tag="VFH60_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312967.1"
                     /translation="MAQNMMWNEIEAQYRGQATGGDVVTVAEDAMRLAHKKAGNWAFE
                     ADWWVETLVRVLEHWGLYSKRWIEPSTQVVRLHFTLGEPKTAAPGATGPLSARIMSGP
                     LDRNKDKPGS"
     gene            complement(1287..>2221)
                     /locus_tag="VFH60_03965"
     CDS             complement(1287..>2221)
                     /locus_tag="VFH60_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET6312968.1"
                     /translation="KLAIVGPVNAGKSTLFNLIEGKDVSEVSPVPGTTKVAIEQKMGP
                     FVLVDTPGFGEVGGVDRANVAGLSAQSADAVLLLLDAGAGLRQGDADLLRYLQGLRRP
                     VVVALNKIDLLGKDADKVAADASQRLGLSVIPVSAKKGTNVAEKLLPALVDAMPDLAV
                     ALGRELPKVRRAMAGKIVKQSSILNTAVGAEPIPFIDIPVLLATQSRMVLRIAAIYGE
                     PFTAKHAKELISTIAGGLAFRFLAQQGAKLVPTGGWAVAAGIAAVGTWAMGQVAIEYF
                     ESAKRLNKQQLRDMYKNALKRNQETFEQQGQPRP"
BASE COUNT          387 a          763 c          672 g          399 t
ORIGIN      
        1 tctacccggc gtctacacat ttacgggcct catagtgggg cgagtgtacg cgatagagga
       61 ccccgacggg ctgaccatca tcgacgcagg tttgccggtc gccccctcgc gcatcgaggc
      121 ccaacttcgg gcggcggggc acgagccggg cgacgtgaag cgcatcctag tcacgcacgc
      181 ccaccccgac cacgtaggcg ggcttcccgg actgaagaag ctcacgggcg cggaggtcat
      241 ctgctcggag ccggaacgcc cggtggtcga gggcaaggtg gctatagcca tgatgtcccc
      301 ctacaaggag atgcaggagt cgcgccgctt caagccgcct cccaccatgt tggaggctac
      361 tcccgtggac cgcacggtgg gtgagggcga cgtgctcgat gaggtgctgg gcggcctcca
      421 ggtgctcatc acccccggcc acgcgctcgg ccatatcacc ttctggcagc cggagcgccg
      481 catcgccttc tgtggcgacg tgatgatgca cctcgccggg ctgaccctcc ccttcggccc
      541 cttcaccccc gacatggacg agaaccgccg ctccatccgc cgcatagcct gcctcgaacc
      601 cgaagccgtc ctgttcggcc acggccagcc cctcctcaaa gacaccgccc gccgcgtgca
      661 agccttctcc gaccgtgtca acaggccgtg agattagaga cgaaaccgca aagacgcaaa
      721 gacgcaaaga acgcgcagag cttaggattg ttgttctaag tacaaaaagg accagagtac
      781 atcacttcgt accctgctcc ttttgtccca tcgaacccat ttcttcactt tgcgcgtcct
      841 ttgcgtcttt gcgtcttagc gttttcgtct ctttccacgc tatgtcctgg tataatgggc
      901 gtgtagtggg aggttagctg ctaggcggag gcaggttagt atggctcaga acatgatgtg
      961 gaacgagatc gaggcccagt accgcgggca ggctacaggg ggagatgtgg tgacggtcgc
     1021 ggaagacgcg atgaggctgg cccacaagaa ggcgggcaac tgggcgttcg aggccgactg
     1081 gtgggtggag acgctcgtgc gcgtgttgga gcactggggc ctctacagca aacgctggat
     1141 cgagccgtct acccaggtcg tgcgcctcca ctttacgctc ggtgagccta agaccgccgc
     1201 gccaggggct acgggtccgc tctcggcccg catcatgtcc ggccccctgg accgcaacaa
     1261 ggacaagccc ggctcctgat acgctcttag ggccggggct ggccctgctg ctcgaacgtc
     1321 tcctggttcc tcttcagcgc gttcttgtac atatcgcgta actgctgctt gttcagacgc
     1381 ttggcgctct cgaaatactc tatcgccacc tgccccatcg cccacgtccc cacggccgcg
     1441 atgcccgccg ctaccgccca accgcccgtg ggcaccagct tggccccctg ttgcgccagg
     1501 aagcgaaagg ccagcccgcc cgcgattgta gaaattagct ccttggcgtg cttggcggtg
     1561 aacggctcgc cgtagatggc cgctatccgt agcaccatgc gcgactgcgt agccagcagc
     1621 accgggatgt cgatgaacgg gatcggctcg gcgcccaccg ccgtgttcag tatggaagac
     1681 tgcttcacta tcttgcccgc catagcccgg cgcactttgg gtagctccct gcccagggcc
     1741 accgcaaggt cgggcatggc gtccacgagc gcgggcagca gcttctcggc cacgttggtg
     1801 cccttcttcg cagagaccgg tatcacggag agacccaggc gttgcgaggc gtcggcggcc
     1861 accttgtcgg cgtccttgcc cagcaggtcg atcttgttca gggccaccac cacgggcctg
     1921 cgcagcccct gcaggtagcg cagcaggtcg gcgtcgccct ggcgcagccc cgcgcctgca
     1981 tccagcagca acagcacggc gtcggcggac tgggcgctaa ggcccgcgac gttggcgcgg
     2041 tccaccccgc ccacctcacc gaagccgggc gtatccacca gcacgaacgg ccccatcttc
     2101 tgctcgatag ccaccttggt cgtaccgggc accgggctta cctccgacac gtccttcccc
     2161 tcgatcaggt tgaacagtgt agacttcccc gcgttcacag gccccacaat agccagcttg
     2221 g
//
LOCUS       DASRWZ010000255         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_431396, whole genome shotgun sequence.
ACCESSION   DASRWZ010000255 DASRWZ010000000
VERSION     DASRWZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431396"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..2333
                     /locus_tag="VFH60_03970"
     CDS             129..2333
                     /locus_tag="VFH60_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312969.1"
                     /translation="MKRLASWAPYMALGLALLAALLDLPNRVASVADTLPVVRLLDQP
                     DEQYRLHLGEVPYDLLRAADAALPPDATVLLVTPGRDTGTLEYITYHRALYFLTPRPV
                     WWAAPTPPDGTWKSRWWLSTPLTPEALRAVAAGKGASHMLVFGVSEAAPFERPMLVAA
                     DGYLSQLDAQPPGPTTQPAPSTYAGPLWPLGVLLAVAVPMLLGGLALVAAGRLGYRVR
                     GVEALSVAWSLGAGLLTLLMLYLNALGFSLDRQMAIAIVVGGIATLPVSRAVLRGSRP
                     VRASGEASQVKSLTGNESEAQAGSTRLLLPAYLARGVQYLLVALLALQFVLVAVPALG
                     RPLDVWDSWVNWGIKSRAIFLEGHISSAVYADPSRAVTHLDYPLLVPLLQSWFYGWAG
                     APDDRLAGVASVLFYVALLGICYAAVRARGGNCLLALAVAATVGTISHLAGLASIVFA
                     EMPLAVFATIAGVYLLKWLDGGPRGTLLVSALGAGLLCWTKRDGVLFLGALLPAVVLL
                     GRWHRRAWVGAGALVVGALLVSGPWYAVAAAHSFPAPDFQPAGLDTLAANLGRWSTIM
                     ELEWHSLTSQGWSYIWLLAVLSGLVYWVVGRGGDAGDGLRGALWLKPRAYKRQSPLRR
                     LPSSSSVCEGDFASSQAATLLARLDQPDEDDSTPEGKAAWAALIPVAALLHIVLSGGV
                     YFFSAFVPFEQHILSSIDRLLAQVAPLVVLWVAFQALPESVSSRYPAKLSSE"
BASE COUNT          375 a          813 c          833 g          556 t
ORIGIN      
        1 cgcaccctca gagttgctat gacgttgcgg ggcaacgata agctgaatcg ctcccattat
       61 atccccggcg tcaggaccat gggcaaccgg agcgttgtgc tataatcccg cccatgaagg
      121 ccgtcgatat gaagcgactc gctagctggg caccatacat ggcgcttggg cttgctttgc
      181 tagcagccct attggacctg cccaatcgtg tcgcgtcggt tgccgatact ctgccggtgg
      241 tgcggttgct tgatcagccg gacgagcagt accgcctcca cctgggggag gtgccttacg
      301 acctgctgcg ggccgccgac gccgctttgc cccccgatgc cacggtgttg ctggtgacgc
      361 cagggcgcga caccggcacg ctggaataca tcacctacca ccgcgccctc tatttcctca
      421 cgccgaggcc ggtgtggtgg gccgcgccaa cgcctcctga tggcacctgg aagtcgcgct
      481 ggtggctgtc caccccgctt acaccggagg ctctacgtgc ggtagctgcc ggcaagggtg
      541 cctcccatat gcttgtgttc ggcgtatctg aagctgcgcc atttgagcgc cctatgctcg
      601 tggcagcgga tggttacctg tcgcaactgg acgcacaacc gcctggccca acaacgcaac
      661 ctgcgccttc gacctacgca ggacctttgt ggccccttgg ggtcttgctg gcggtagccg
      721 tgcccatgct gcttggcggg ttggcgctcg tggccgccgg tcggctgggc tatcgcgttc
      781 gcggcgtgga ggcgttatcg gtggcctggt cgctgggcgc cgggctgctc actctgctga
      841 tgctctactt gaatgccctc ggattcagtc tggaccggca aatggcaatt gcgatagtgg
      901 ttgggggaat tgccacgttg cctgtaagta gggccgtgct acgtgggagc cgccctgtca
      961 gggcgagtgg agaagcctcg caggtaaagt cgcttaccgg caacgaatct gaggcgcagg
     1021 caggtagcac tcgcctactg ctgcccgcgt acctggctcg tggggtgcag tacttgctgg
     1081 tggctttgtt ggcgctgcaa ttcgtgctgg tcgccgtacc cgcgctgggc aggccgctcg
     1141 acgtgtggga tagttgggtg aactggggta ttaagtcacg cgctatcttt cttgaggggc
     1201 acatctcgtc cgccgtttac gccgaccctt cccgcgccgt cacccatctc gactacccgt
     1261 tgctggtgcc cctgctgcaa agctggttct acggctgggc gggtgccccc gacgaccggc
     1321 ttgcgggggt ggcctcagtg cttttctacg tagcgttgct gggtatctgt tacgcggcgg
     1381 tgcgcgcacg tgggggtaat tgccttctcg ccctggcggt tgccgccacg gtaggcacaa
     1441 tctcgcacct ggcgggcctg gcaagcatcg tgttcgccga gatgccgttg gcggtattcg
     1501 ccaccatcgc gggggtctac ctgctcaagt ggctggatgg cgggccgcgc ggcaccctgc
     1561 tggtatcggc gctgggcgcg ggcttactgt gctggaccaa gcgcgacggc gtgcttttcc
     1621 tgggggcgtt gctcccggcg gttgtgctac tcggccggtg gcaccggcgt gcgtgggtgg
     1681 gtgcgggtgc cctcgtagtg ggcgcgctac tggtgtccgg cccctggtac gccgtcgccg
     1741 ccgctcatag cttccccgct cctgacttcc agcctgccgg cctcgacacc ctggccgcca
     1801 acctgggccg ctggagcacc ataatggagc ttgagtggca cagcctcacg agccagggtt
     1861 ggagctacat atggctccta gcggtcttga gtgggctggt gtattgggtg gtaggtcgcg
     1921 ggggtgacgc cggggacggc ctgcggggtg ccctgtggct aaagccacgg gcttacaaga
     1981 ggcaaagtcc ccttcggaga ctgccctcaa gttcctcagt ctgcgaaggg gactttgctt
     2041 cttcccaggc cgctactttg ctggcacggc tagaccaacc cgacgaggac gacagcacgc
     2101 cggagggtaa ggccgcgtgg gcagccctca tccctgtagc ggcgctcctg cacatcgtct
     2161 tgtctggcgg ggtctacttc ttctcggcct tcgtgccctt cgagcagcac atacttagct
     2221 ccatagaccg gctgctagcc caggtagccc ccctcgttgt gctctgggta gcttttcaag
     2281 ccctgcctga atctgtcagc agccggtatc cagcgaagct gagcagcgaa tgagcagctt
     2341 tttgcctgta aagcgcgcac tgcttagtcc cctgcgggga ctttgctact tctcagcccg
     2401 tggtttagcc acagggcatc ccattaggcc gctgcccctg ctattcgagc gtgttgtaca
     2461 cgaaccccaa cacgcccggc cccgtatgtg tcgaaataac cgggcctaat tccccgaaga
     2521 attcttcctg gacgttgagc cgccctcgca ccatttcagc cagggagcgg gcatcgg
//
LOCUS       DASRWZ010000256         1807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_911715, whole genome shotgun sequence.
ACCESSION   DASRWZ010000256 DASRWZ010000000
VERSION     DASRWZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911715"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1793)
                     /locus_tag="VFH60_03975"
     CDS             complement(<1..1793)
                     /locus_tag="VFH60_03975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6312970.1"
                     /translation="MTTQPIKTIPVARRRLMLGLWLLPLLLGLVWILLSYHLVREQAV
                     DVGNAGDAAFISGGFHSDEADLTYRYRWTRGRASITFHGAGSARPTEVLVQAQGFRPE
                     GFPQVVTMTVSVEGSVIPLEPSVVTLTNSLETYRFGLSGPTPIQPYTVVLDTPTFNAP
                     GDPRPLGAKIARVELKQRGDGLNLPPLDALLWPLVLIGGLAYLLLQLPSLLGYFLVLP
                     ATAVVLAVVMLYRLYAAAYLPPLAFVVGLAALLHWQRARLAGWPEWVDRLGKVVNAQR
                     VMVAAIATYALVSLWVLPQVDWIGHADYAENAVIARNFVQGKGLTVDYVAQFYEPHPD
                     LSHPAETWPLLQPLTIAPFFALFGPVTWVAKLPNLLVMLALAWLVFSLATRLWDARVG
                     LLAGLFTLAHPYFFNSVLYPINDLGFALIFFALAWLVWHALSPYHDISPEDTRDEGLV
                     QVERSEGRRERLLVAVGVGVLAGLLVWSKPSGAILLLGLLLWAGWRWLRLRRLHSRGL
                     EWRALLPMLFAFVLVLAPFFLRNMLAFGKPFFTTEGYDAWILRYWPGHNWEDIYKYYV
                     GGELPNFRWVLGGKFGYETLFQAIGLNLQWV"
BASE COUNT          379 a          589 c          583 g          256 t
ORIGIN      
        1 cacacccatt gcaggttcag tccaatcgcc tgaaacagcg tctcgtagcc gaacttgccg
       61 ccgagcaccc acctgaagtt gggcaattcc ccgcctacat aatacttgta aatgtcctcc
      121 cagttgtggc cgggccagta gcgcaggatc caggcatcat aaccttctgt ggtgaagaac
      181 ggcttgccga aagccagcat gttgcgcaag aagaaagggg caagcaccag cacgaacgcg
      241 aacagcatgg gcaggagggc acgccactcc aggccccggc tgtggagcct gcgaagccgc
      301 agccagcgcc accccgccca cagtagcaag cccagcagca ggatagcccc gctaggcttg
      361 ctccagacca gcagcccggc gagcacgccc acccccacgg ccacgagcag ccgctccctc
      421 cgcccctcgc tccgctctac ctgcaccagc ccctcgtccc gcgtatcttc gggggatatg
      481 tcgtgatagg gggagagggc gtgccacacc agccaggcaa gcgcgaagaa gatgagcgcg
      541 aaaccgaggt cgttgatcgg gtagagcacc gagttgaaga agtaggggtg cgccagggtg
      601 aagagacccg ccagcagccc cacccgcgca tcccacaggc gcgtcgccag ggaaaagacc
      661 agccaggcaa gcgccagcat caccagcagg ttgggcagtt tcgccaccca ggtgacgggg
      721 ccgaacaggg cgaagaaggg cgcgatggtg aggggttgaa gcaggggcca ggtctcggct
      781 gggtgggaga ggtcggggtg cggctcgtag aactgggcca cgtaatcgac ggtgaggccc
      841 ttcccttgca cgaagttgcg cgcgatcacc gcgttctcgg catagtcggc gtgccctatc
      901 cagtccacct gcgggagcac ccaaagcgac accagcgcgt acgtggctat cgccgccacc
      961 atcaccctct gcgcgttaac caccttgccg aggcggtcca cccattccgg ccagcctgca
     1021 agccgcgcgc gctgccagtg tagcagggcc gccagcccaa ccacgaacgc cagcgggggc
     1081 aggtaggcgg cggcatatag cctgtagagc atcaccacgg caagcactac cgcggtggcg
     1141 ggcagcacca ggaagtagcc cagcagagaa gggagttgca gcagcaagta cgccaacccc
     1201 ccgatgagca cgagcggcca caagagggca tccaggggcg gcaagttcaa cccatcgccc
     1261 ctttgcttca actccacccg cgctattttc gcaccgagag gccggggatc gccgggtgcg
     1321 ttgaaggttg gggtgtccaa caccacggtg tagggctgga taggagttgg gccggatagc
     1381 ccgaacctgt acgtctcaag gctgttcgtg agggtgacaa ctgaaggctc cagcgggatc
     1441 accgagcctt ccaccgaaac ggtcatagtg accacctggg gaaagccttc gggccggaag
     1501 ccctgcgctt ggacgagcac ctccgtgggc ctcgcgctac cggctccgtg gaacgtaata
     1561 gaggcacgcc cgcgcgtcca gcgatagcgg taggtcaggt cggcctcgtc cgagtgaaag
     1621 ccgccgctga tgaaggcggc gtcgcctgcg ttgcccacgt ccacggcctg ctcgcgtacg
     1681 aggtggtagg agaggagtat ccacaccagg cccaggagca aaggcaagag ccacagcccc
     1741 agcatgagcc ttcgccgggc cacggggata gtcttgatgg gctgggttgt catctcggcg
     1801 gctcctg
//
LOCUS       DASRWZ010000257         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_979846, whole genome shotgun sequence.
ACCESSION   DASRWZ010000257 DASRWZ010000000
VERSION     DASRWZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979846"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFH60_03980"
     CDS             <1..1180
                     /locus_tag="VFH60_03980"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312971.1"
                     /translation="GAILVLLVARFAPARRVREVLGLMGALFGISCALIGQTSRFWLR
                     SFTETGSTDTEQFLGGLRGIIAWPLPPFMAGRGLEAAGRGDIVGALANLSVFLLLTFG
                     VFALCVWAADFLYAAGWVRMQSSGSARRSKQRADKAARERGWLSRVPADVAIAIKDWK
                     VVPRDLRNFAQMLAPLIIWPILYLNFTSGGRRGRGVPGADFFSNLDGVMMAVGVLIST
                     VGLMSNIAFTSISREGKSWWLLKIAPVSAFELLRGKFLAAAAPYVVLSTLLMLGAAIW
                     RQLDPLWSLYGWLGVEVLGLGMLAVAVALSVPWAKLDWDDPRKMTSGWGSFAAFVIWF
                     GMGLLGGLFLALPVFFEALDPLLAPLLALLGLICATGMSAGVAYLAYKYGERKLPEIG
                     "
     gene            complement(1244..1453)
                     /locus_tag="VFH60_03985"
     CDS             complement(1244..1453)
                     /locus_tag="VFH60_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312972.1"
                     /translation="MTLDALKDQHPQEGLLRDEETGHYRILPGFGHQAHRYAMHETWL
                     DEDDAMWIDGKAVERESHKAEGYGQ"
BASE COUNT          205 a          456 c          512 g          328 t
ORIGIN      
        1 gggtgcgata ctggtgctcc tggtggcgcg tttcgcccct gcccgccgcg tgcgcgaggt
       61 gctgggcctc atgggcgcgc tgttcggcat ctcctgcgcc ctcatcgggc agacctcgcg
      121 cttctggctg cgctctttca ccgaaaccgg cagcaccgac acggagcagt ttcttggggg
      181 tttgcgaggg ataatcgcct ggccgctgcc tccgttcatg gcggggcgcg ggcttgaggc
      241 cgccgggcga ggggacatcg tgggggcgct cgctaacctg tcggtgtttc tgctgctaac
      301 gttcggggtg ttcgccctgt gcgtgtgggc cgccgacttc ctctacgcgg cgggctgggt
      361 gcgcatgcag agcagcggca gcgccaggcg cagcaagcag cgggcggaca aggcggcaag
      421 ggagcgcggc tggctgtcgc gggtgcccgc cgacgtggct atcgccatca aggactggaa
      481 ggtggtgccc cgcgacctgc gcaacttcgc ccagatgctc gcgcctctca tcatctggcc
      541 gatcttatac ctgaacttca cgtcaggcgg cagacgcggg cggggcgtac ccggcgccga
      601 cttcttcagc aacctcgatg gggtgatgat ggcggtgggg gtgctcatct cgaccgtggg
      661 gctgatgagc aatatcgcct tcacgagcat cagccgggag ggcaagtcgt ggtggctgct
      721 gaagatagcg cccgtgtcgg cgttcgagtt gctgaggggt aagttcctgg cggcggcggc
      781 gccctatgtg gtgctgagca cgctgctgat gctgggggct gccatctggc ggcaattgga
      841 cccgctatgg agcctctacg gctggctggg cgtcgaggtg ctgggcctgg ggatgctggc
      901 ggtggcggtc gccctgtcgg tgccgtgggc caaactcgac tgggacgacc cccgcaagat
      961 gacctcaggc tggggcagct ttgccgcttt cgtcatctgg ttcgggatgg gcctcctcgg
     1021 cggcctcttc ctcgccctcc cggtcttctt cgaggccctg gaccccctgc tcgcccccct
     1081 gctggccctc ctgggcctga tatgcgccac cggcatgagc gcgggagtgg cctacctcgc
     1141 ctacaagtac ggcgagcgca agctgccgga gattgggtga attacgaatt acgaattaca
     1201 gaggaattac cgtaattcgt aattcgtaat tcgtaattcg taattactgc ccgtagcctt
     1261 cggctttgtg gctttcgcgc tctaccgctt tgccgtctat ccacatggca tcgtcttcgt
     1321 ccagccaggt ctcgtgcatt gcgtagcggt gggcctggtg gccgaagccg ggcaggatgc
     1381 gatagtggcc ggtctcctca tctcggagca gcccctcttg cgggtgctgg tcctttaggg
     1441 catctagagt catcgctttc ctcctaatct acgatagtgt gctgtatgcc attctttatt
     1501 c
//
LOCUS       DASRWZ010000258         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_775003, whole genome shotgun sequence.
ACCESSION   DASRWZ010000258 DASRWZ010000000
VERSION     DASRWZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775003"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFH60_03990"
     CDS             complement(<1..849)
                     /locus_tag="VFH60_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6312973.1"
                     /translation="MSDTHRTIEAVWRMESAKLIGALARIVRDVGLAEELAQDALVIA
                     LERWPEVGVPDKPGAWLMATAKHRAIDRLRHLRLAERKHEELGRELELRHDLTEADFE
                     AVLDEGDHVQDDLLRLIFTACHPVLSPEARVALSLRLLGGLTTPEIARAFLVPEATVA
                     QRIVRAKRTLAEARVPFEVPSGAGRAARLASVLEVIYLIFNEGYSATAGDDWMRPALC
                     EDALRLGRILAGLMPDEAEVHGLVALMELQASRVRARTGPSGAPVLLLDQNRRRWDQL
                     LILRGLA"
     gene            complement(877..1350)
                     /locus_tag="VFH60_03995"
     CDS             complement(877..1350)
                     /locus_tag="VFH60_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6312974.1"
                     /translation="MQKITTFLTFNDQAEEAVNLYVSTFRDSQVTNISRYGERAPFPA
                     GTVMTASFVLAGQEFVALNGGPSFTFAEGISLYVNCETQEEVDELWEKLSEGGEQGPC
                     GWLKDRFGVSWQIIPAALGQLLQDEDPRRAGNVMQAMLEMTKIDIAGLRRAYEQA"
     gene            complement(1452..1877)
                     /locus_tag="VFH60_04000"
     CDS             complement(1452..1877)
                     /locus_tag="VFH60_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6312975.1"
                     /translation="MRFMVIVPAGNGSEEGILPDEKLLAEMGNFNEEMVKAGVMLAGE
                     GLHPTSKGARLEFTGGTTRVIDGPFTESKELIAGFWLIQVKSKEEAIEWMKRAPFGEG
                     VVLEIRQVFETEDFGPAMTPELREQEERLRAQTEANAKS"
     gene            2447..>2876
                     /locus_tag="VFH60_04005"
     CDS             2447..>2876
                     /locus_tag="VFH60_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312976.1"
                     /translation="MKRPLPGLALRAALALVLVLVPLRASTLETSFAQTTVVQFPATG
                     KQLKGKFLEYWNTHGGLPQQGYPISDEFPEVSTTDGKTYTVQYFERAVFELHPENAAP
                     NDVLLSLLGVSSYKQRYPQGAPGQAPNTSPGSVAFEQTGHR"
BASE COUNT          516 a          944 c          839 g          577 t
ORIGIN      
        1 cgctagccct cgaaggatga gaagttggtc ccaccgccta cggttctgat cgaggagcag
       61 tacgggcgca cccgacggcc ctgtgcgcgc ccgcactcgc gaggcctgta gctccatgag
      121 cgcgaccagg ccgtggacct cggcttcgtc cggcataagc cctgccagga tgcggcccag
      181 gcgtagcgca tcctcgcaca gcgcgggccg catccagtcg tcgcccgccg ttgccgagta
      241 gccctcgttg aagatgagat aaatcacctc cagcaccgaa gccaggcgag ccgcgcgccc
      301 ggccccgctc ggcacctcaa acggcactcg cgcctccgcg agggtccgct tggcccgcac
      361 gatgcgctgg gcgacggtgg cctccggcac gagaaacgcg cgggctatct ccggggtggt
      421 caggccccct agcaaccgga gcgagagggc cacgcgcgcc tcgggcgaga gcaccgggtg
      481 gcaggccgtg aagatcaggc gtagcagatc gtcctgcacg tggtcgcctt cgtcgagcac
      541 ggcctcgaag tcggcctcgg tcaggtcgtg gcggagttca agctcgcggc cgagttcttc
      601 atgcttgcgc tcggccagcc tgaggtggcg cagccggtcg attgcacggt gtttcgcggt
      661 ggccatgagc caggcacccg gcttgtcggg gacgcccacc tccggccacc gctccagcgc
      721 gatcaccagc gcgtcctggg ccagctcttc agccagccca acgtcgcgca cgatccgcgc
      781 cagagcgccg atgagctttg ccgactccat ccgccagacg gcctcgattg tgcgatgagt
      841 gtcggacatg gtctaagcta ccgtacagag gccgggttat gcttgctcgt acgcccgcct
      901 caagcccgcg atgtcgatct tggtcatctc caacatcgcc tgcatgacgt tcccggccct
      961 gcgggggtct tcgtcctgca acaactgtcc cagtgcggcg gggataattt gccatgacac
     1021 gccaaacctg tctttcagcc agccgcatgg gccttgttcg ccgccctccg agagcttctc
     1081 ccaaagctcg tctacttctt cctgcgtctc gcagtttacg tagagcgaga tgccttcggc
     1141 gaaggtgaaa ctcggccccc cgttgagcgc cacgaactcc tgccccgcga gcacgaacga
     1201 cgcagtcatt accgtcccag cgggaaatgg tgccctttca ccatagcggc tgatattcgt
     1261 gacttgtgaa tccctgaaag tggaaacgta gaggttcacc gcttcttctg cctgatcgtt
     1321 gaacgtcagg aacgtggtga ttttttgcat ggtgttctcc tagcttgtaa tggctgcata
     1381 aagaggccct tacccggcat cgctgcgaca tcagcccgat cagaagatgg tcagcgggta
     1441 ggcgcgtcgg actagctctt cgcgttcgct tccgtctgtg cgcgcaatct ttcctcctgc
     1501 tcccttagct cgggcgtcat cgcgggacca aagtcctccg tctcgaacac ctgccgaatc
     1561 tccaggacga cgccctctcc gaagggggcg cgcttcatcc actcaattgc ctcttccttc
     1621 gacttcacct ggatcagcca gaagcccgcg atcagctcct tcgactcggt gaaaggcccg
     1681 tcgataaccc tggtcgtgcc gcccgtgaac tccaggcgcg cgcccttgga ggtggggtgc
     1741 aggccctcgc ccgcgagcat cacgcccgcc tttaccatct cttcattgaa attgcccatc
     1801 tccgccagca acttctcgtc cggtaggatg ccctcttcgg agccgttgcc ggcgggaact
     1861 atgaccatga atcgcatcgc tgtatctcct ttttgcttgt ttgcgtgtta gattcggagc
     1921 ttggagatgc gtctcctggt ctgaccagac caggcgcaaa tcctcgctct atatatacgt
     1981 cgaacgaggt gcggcaaaat cgacatgaga ttcgcgaaat cctcgaaatt gctcaacacg
     2041 aggtgcgggg gcattgaata cgcagacggt accccattgc cggcccgata ttggccggtt
     2101 acacccgagg agcggggaag ggaatgtggg ggccggtgac aagtctacca taatgaggcc
     2161 caccctgcac ccagcccgcg tgagtggcaa cctggagttc cgaaagtgct ccaaaggtgc
     2221 tccgctagat cgtggctgca cgcgttccgg ccctgagtac tccacgtgag gatgtggctt
     2281 tgggctgagt tcttggtgta caatggcccg gcgtgacttc catgcatcca aagccttata
     2341 tttggcctca caggcgggca ggagctaata gcagtaccgc cgtccgtgcg ctctcttatg
     2401 cgagcggtcc catgaaatcg ctctctttac gtgaggacac cacactatga aaagacccct
     2461 acccggtttg gctttacgcg cggccctggc gcttgtattg gtcctggtcc ctctgcgggc
     2521 ttctacattg gagaccagtt tcgcgcaaac cacggtcgtg cagttccccg ccaccggaaa
     2581 acagttgaag gggaagttcc tcgaatactg gaacactcat ggcgggctgc cccaacaggg
     2641 ctatccgatc tccgacgagt tcccggaggt gagcactacc gacggcaaga cctataccgt
     2701 gcagtacttc gagcgggccg tcttcgagtt gcacccggag aacgctgcgc cgaacgacgt
     2761 gttgctctcc ctgctgggcg tgtcgtccta caaacagcgc tacccacagg gcgcgcccgg
     2821 acaggccccg aatacatctc ctggctccgt tgctttcgag cagacgggtc atcgcc
//
LOCUS       DASRWZ010000259         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1186367, whole genome shotgun sequence.
ACCESSION   DASRWZ010000259 DASRWZ010000000
VERSION     DASRWZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186367"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFH60_04010"
     CDS             <1..823
                     /locus_tag="VFH60_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6312977.1"
                     /translation="RLEGVPHVWHVHELFKRVEGRVLTRLINALSWRVVVPSEAVAAN
                     LAQSYPAVRRKLAVIKNGIDATMYRTVSGEQVERLRAEWKIPPGMKVVGMIGRIGMWK
                     GEEQFVELAARLVEQGIDAHFVIVGGVFDDRRHLLDRLEELIARKELRLRMVVAGLRD
                     DVPVAINLFDVLVHLPTRPEPFGLVALEAMSASKPVVAAALGGLVEIVDNGATGFLVR
                     PGDLDGAAEKVSLLLRDGAMSEKMGRAGCERVDTQFLSAHYAAAFGRLYKQLDAR"
     gene            988..1887
                     /locus_tag="VFH60_04015"
     CDS             988..1887
                     /locus_tag="VFH60_04015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6312978.1"
                     /translation="MAVILNWNNLELTQETLRSLYAQDYPHLRAVLIDNASRNQEETL
                     SVIHREFPQVELWGSRRNLGFAGGCNLGMRIALDMGAERVLLINNDVLLEPDAVRLLV
                     QALEADERAGAASPLVLYASEPDRVWFAGGKVRQGGRVLPEHLWLDEPPGRVPDVASY
                     ETEWLAGTCVLVRRSAVERAGMLDPAYFLYWEDVDWCYRLRAEGYRLLVAPRAIIRHR
                     VNATTGSLPSLGSVYYWERNRLRFIQKWGTWRSRITALVKIVWRSLAWMVRPPRDDPQ
                     ASTKLEAYRDYLLRRFGQRRAPA"
     gene            1887..>3179
                     /locus_tag="VFH60_04020"
     CDS             1887..>3179
                     /locus_tag="VFH60_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312979.1"
                     /translation="MAIVGIGLSLILLALYAWLLVVIYRAPFRALAVLVAGMAFHNIV
                     LMFLIDLGTPALLLRMVQFWKEGVLLLLAFMAGRLAVRAWRAGKRPRLSWLDWTMLAF
                     AVLSFVYLVIPDWAFPIESSLNQRLVSFRVMLLLPLLYAFGRIFWRYTRPDLIWTGRM
                     ILGSILVVAVFGLWELWFVPTPDWLDWGVNGFTAWLGYGYGGPKGLPENFFQSTDAGY
                     LLRRMVSTYISPLGLAYTGLLLVPICTVLVSSRNVARELPAWFRTAAFVALLVSILFS
                     ITRGALLMMVLEFGLLMLLIRRRRTLVAGGIVAVTVVFILIEYVNFGPLLTFGLEEVR
                     PPLGLAAVRQAKTLAPGQSDSAIATAVASPTQRAGQSNNAGEQDTKGGELVERMLTRE
                     DPSTRGHIEALRVGAIHVAKYPLGTGLGSSVPRFGSAEG"
BASE COUNT          534 a          917 c         1105 g          623 t
ORIGIN      
        1 gcgcctggaa ggggtgcccc acgtctggca cgtacacgaa cttttcaagc gcgtagaggg
       61 gcgggtgctc acgcggctca taaacgcgct gtcgtggcgg gtggtagtgc cctccgaggc
      121 agtagccgcc aacctggcgc agtcataccc ggcggtgcgg cgcaagctgg ccgtcatcaa
      181 gaacggcata gatgctacaa tgtaccgcac ggtgtccggc gagcaggtag agcggcttcg
      241 tgccgagtgg aaaatcccgc ccggcatgaa agttgtgggt atgatagggc gtatcggcat
      301 gtggaagggc gaagagcagt tcgtggagct ggctgcccgc ctggtggagc agggcatcga
      361 cgcgcatttc gtgatcgtgg gcggggtgtt cgacgaccgc aggcacctgc tggaccgcct
      421 tgaagagttg atagcgcgca aggagctgcg gttgcggatg gtggtcgcgg gattgcggga
      481 cgacgtgccg gtggcgatta acctgttcga cgtgctggtg cacctgccga cgcgccccga
      541 gccgttcggc ctggtggcgc tggaagcgat gtccgcctca aagccggtag tggcagcggc
      601 attgggagga cttgtcgaaa tagtggataa tggcgcgacg ggtttcctag tgcggccggg
      661 cgacctggac ggtgcggcag agaaggtaag tctgctcctg cgggacggcg ctatgtccga
      721 gaagatgggc cgggccggtt gcgagcgggt ggacacgcag tttctttcag cacactatgc
      781 cgccgcgttt ggccggttgt acaaacagct agacgcccgc tgatgccggt aggtcgcctg
      841 cgcaccgcac cggccgggtt gacctctgcc cttagggtac atacagcgtc tatgcatgcg
      901 ccagacaaca tagtcatgac gaatggccgg tctctggcct cctctccctt acaccacacc
      961 tctcccatca cgcatacacc tggggtggtg gcagtcatcc tcaattggaa caacctggag
     1021 ctgacccagg agaccctgcg ttcgctatac gcacaagact acccacacct gcgggcggtg
     1081 ctgattgaca acgcttcccg gaaccaggag gagacgctga gcgttatcca tagggaattc
     1141 ccccaggtcg aactgtgggg aagcaggcgc aacctgggct tcgcgggggg ctgcaacctg
     1201 ggaatgcgga tagcgttgga tatgggggcc gaacgcgtgc tgctgatcaa caacgacgtg
     1261 cttttggagc ctgacgctgt acggctgctg gtgcaagccc tggaagcaga cgagcgcgcc
     1321 ggggccgcaa gcccgctcgt gttgtacgct tcggaacccg accgggtctg gttcgcgggc
     1381 ggcaaggtgc ggcagggggg gagagtgctg ccggagcacc tgtggctcga cgagccgccc
     1441 ggccgcgtgc ccgatgtggc ctcatacgag acggagtggc tggcgggcac ctgcgtgctc
     1501 gtgaggcgtt cggcggtgga gcgggcaggt atgctggacc cggcttactt tctctactgg
     1561 gaggacgtag actggtgcta caggttgcgc gctgaggggt accgcctgct ggtagcgccc
     1621 cgcgccataa tccggcaccg ggtgaacgca acgacgggca gtttgccgtc gctgggcagc
     1681 gtttactact gggagcgtaa ccgcctccgt ttcatacaga agtggggtac gtggcgctcg
     1741 cgcatcacgg ccctggtaaa aattgtttgg cggtcgctgg cctggatggt gaggccgccg
     1801 cgagatgacc cgcaagcctc gacgaaattg gaggcgtacc gggactatct tttgaggcgc
     1861 ttcggacaga ggagggcacc ggcttagtgg ctatcgtcgg tatagggttg agcctcattc
     1921 tgctggcact gtacgcgtgg ctgctggtgg ttatttaccg cgcgccgttc agggcgctcg
     1981 cggtcctcgt cgcgggtatg gccttccaca acatcgtcct catgttcctg atcgacctcg
     2041 gcacccccgc gctgctgctc aggatggtgc aattctggaa agagggtgtg ctcctcctgc
     2101 tggccttcat ggcgggcagg ttggccgtgc gggcgtggcg cgcggggaag cgtccgcgtc
     2161 tctcctggct cgactggacc atgctggcat tcgccgtgct gtccttcgta tatttggtga
     2221 tacccgactg ggccttccca atagaaagca gcctgaacca gcgcctggtg agcttccgcg
     2281 tcatgttgct cctaccgctc ctgtacgcct ttgggcgaat cttctggcgg tacacgcgcc
     2341 cggacctcat ctggacgggc cgaatgatac tcggttcgat cctggtggtg gcggtgttcg
     2401 ggctgtggga gctttggttc gtgcccacgc cggactggct cgactggggt gtgaacggct
     2461 ttactgcgtg gctcggttac gggtacgggg ggccaaaggg gctaccggag aacttcttcc
     2521 agagcaccga cgcgggctac ctgctacgcc gcatggtctc cacctacatc agcccactcg
     2581 ggctggcgta taccgggctg ttgctggtgc caatctgcac cgtgctggtc agctccagga
     2641 acgtggcgcg cgagttgccc gcgtggttcc gcacggccgc gttcgtggcg cttttggtta
     2701 gcatattgtt cagtatcacg cgtggcgcgc tcctcatgat ggtattggag tttggcctgc
     2761 tgatgctact catacgccgc agacgtacgc ttgtcgcggg cggtatcgta gcggtgaccg
     2821 tggtattcat cctgatcgag tacgtcaatt tcggccccct cttgacgttc ggcctggaag
     2881 aggtgcgtcc gccgctggga ctggcggccg tcaggcaagc caaaaccctg gctccgggcc
     2941 agtcagacag cgcaatcgcc acggcggtgg cctctcccac ccagagggcg gggcagagta
     3001 acaatgcagg agagcaggat accaagggcg gggaactcgt agaacggatg ctgaccaggg
     3061 aagacccctc gacgcgcggc cacatcgagg ccctgcgggt cggggcgatc cacgtggcaa
     3121 agtacccact cggcacaggg ctgggtagct cagtgccacg cttcgggtcg gcggaaggg
//
LOCUS       DASRWZ010000260         3917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_295656, whole genome shotgun sequence.
ACCESSION   DASRWZ010000260 DASRWZ010000000
VERSION     DASRWZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295656"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..672
                     /locus_tag="VFH60_04025"
     CDS             79..672
                     /locus_tag="VFH60_04025"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HET6312980.1"
                     /translation="MLEVQTEQQTNDQAEDGGLHPLITVAGALLDLDRIKIVAALANG
                     PASRMQLHEATGLAHRDLLRHMDNMQQAGLVKPVGDVRQPDVYTPYELDLKAFSDARK
                     AMGKYKGVRARPTDARELVLDTFMRGGKLSAFPKKHEQTVIILEEIARKFEPEKQYSE
                     RDVNVILEEVNADYCTLRRSLVDYGYLNRSGGIYSKA"
     gene            813..1790
                     /gene="ispH"
                     /locus_tag="VFH60_04030"
     CDS             813..1790
                     /gene="ispH"
                     /locus_tag="VFH60_04030"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET6312981.1"
                     /translation="MRSKEMGMCFGVKMTLDQIDEAGKSRSRIDTLGTLVHNPQLVEK
                     LQGQGVEVVKGLDQTTASTVAVTAHGAAPEVYARAQMQGLELIDTTCPLVTRVQQLAR
                     QQMEAGNWVILYGDAEHPESKGILGWANSVLDEMKAQAESGEQADGDKPKTKVKSWNA
                     RRAISAYSVADIEAALAADGTPTAKIRRIALLSQTTQNTEWFKKFAKEVIDRFLEHGT
                     EVHVHNTICLPTAKRQPAAVDLAKEVDVMVVVGGYESANTRHLRDISSEFCPAYHIER
                     PEQLEEAWFQGVKKVGLTAGASTPDYVINEVEEALHDMNERLARQVATA"
     gene            complement(2038..2655)
                     /locus_tag="VFH60_04035"
     CDS             complement(2038..2655)
                     /locus_tag="VFH60_04035"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6312982.1"
                     /translation="MDPKWLEWVRRLQAIAQNGLTYAQDPYDRDRYNALLDIAAEIVA
                     SHEPVSAEKVRGIFARETGHATPKVDVRGVVFQDDRVLLVKEIADGRWTVPGGWADIG
                     DSPAGATLREIREESGYEARIVKLMALYDRLRHEHPPHAFHIYKVFFLCEITGGAPTT
                     SNETDGVGFFPLDDLPTLSVGRVTAKQLHRFYEHYRDPSLPADYD"
     gene            complement(2661..3116)
                     /locus_tag="VFH60_04040"
     CDS             complement(2661..3116)
                     /locus_tag="VFH60_04040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6312983.1"
                     /translation="MEATNPNYKSAVREIFERAAFVNDVGIRLAECGPGWCESVLAVS
                     PRHYQHHDFVHAGVQATIADHTAGAAATTLIRDTELVLSAEFKINLLNTARGETLKCR
                     AEVLKAGRMLIVVESEVFARSGEKERLVAKATVTLAVVARPGAPAGEAG"
     gene            complement(3170..>3917)
                     /locus_tag="VFH60_04045"
     CDS             complement(3170..>3917)
                     /locus_tag="VFH60_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET6312984.1"
                     /translation="PANHFTLRIFTPGKELPLAGHPPVGASFVIARERMFDRSGDEAE
                     LRLEEGVGLIKVRLEFRDGAPDMIWMTQLPPRFGPVIEDVAAVAEMLSIAPGDLNTDL
                     PVEVVSCGVPFLIVPVRDLAAMRRLRLRQEVSSRLLGPPGVGEVFIFAPEVEYAGSTV
                     HSRMFAPDFGIGEDPATGGASGPLGSYMVHHGLVPADPTAYIVSEQGVEMGRPSFIHI
                     QITREGGEITRVQVGGQVVYMGQGEIELDD"
BASE COUNT          777 a         1246 c         1227 g          667 t
ORIGIN      
        1 cgcctgcctg gtatgattct attgtggggt attccgggca tttagcatgg cgcaggccca
       61 cgagagcgag aagaaacaat gttagaagta cagacagaac agcaaacaaa cgaccaagcc
      121 gaagacggcg gtctgcatcc cctcatcacg gtggcgggcg cgttgctcga cctggaccgc
      181 ataaagatcg tggcggccct cgcaaacggt ccggccagca ggatgcagct acacgaggcg
      241 accggcctgg cccaccgcga cctgctgcgc cacatggaca acatgcagca ggcgggccta
      301 gtaaagccgg tgggcgacgt acgccagccg gacgtttaca cgccgtacga gcttgatctc
      361 aaggcgttca gcgacgcccg caaggcgatg ggcaagtaca agggcgtgag ggccaggccc
      421 accgacgcgc gcgagttggt gctggatacc ttcatgcgcg gcggcaagct ctcggccttc
      481 cccaagaagc acgagcagac cgtaatcata cttgaggaaa ttgcccgcaa gttcgagcct
      541 gaaaagcaat attccgagcg ggatgtaaac gttatattag aggaagtaaa cgcagactac
      601 tgcacactgc gccgctccct ggtagattac ggctacctga acagaagcgg cgggatttac
      661 agcaaggcgt gacgagtgac gagtgatcac gactgagtaa agagacggag acgcaaagag
      721 ggttatcatc agggtgggta ctgctcagtc ctcatcactc gtcactcgtc actcgtcacg
      781 cctaaggaga tggaatagaa atgttttaaa tcatacgctc taaagagatg gggatgtgct
      841 tcggggtgaa gatgaccctg gaccagattg acgaggcggg caagagccgg tctcgcatcg
      901 acacgctcgg cacgctggtg cacaatccgc agcttgtgga gaagttacaa ggccagggtg
      961 tggaggtggt caagggcctc gaccagacga cggcgtccac ggtagcggtg acggctcacg
     1021 gcgcggcccc ggaggtatat gcgcgggcgc agatgcaggg tttggagctt atcgacacca
     1081 cctgccccct cgtgacgcgg gtgcagcagc ttgcccggca gcagatggag gccggcaact
     1141 gggtcatcct ctacggcgat gcggagcacc ctgagagcaa gggcatcctt ggctgggcca
     1201 actcggtgct ggacgagatg aaggcccagg ctgagagcgg cgagcaggcg gacggcgaca
     1261 agcctaaaac caaggtcaaa agctggaacg cccgccgggc catcagcgcc tacagcgttg
     1321 ccgacataga agccgccctc gccgccgatg gcacgcccac cgccaagata cgccgcatcg
     1381 ccctgctctc gcagaccacc cagaacaccg agtggttcaa gaagttcgcc aaagaggtga
     1441 tagaccgctt cctggagcac ggcaccgagg tgcacgtcca caacaccatc tgcctgccca
     1501 ccgccaagcg ccagcccgcc gcggtggacc tcgccaaaga ggtggacgtg atggtcgtgg
     1561 tgggcggcta cgagtcggcc aatacccggc acctgcgcga catctcatcc gagttctgcc
     1621 ccgcctacca catcgagcgg ccggagcaac tggaggaagc ctggttccag ggcgtgaaga
     1681 aggtcggcct caccgccggt gcctccaccc ccgactacgt aatcaacgag gtggaagagg
     1741 ccctgcacga catgaacgag cgcctggcga ggcaagtagc taccgcatag taagaggcgg
     1801 aaccgccaag cacgccaagc acgccaagaa gataaggatt tttcaccgca gagacgcaga
     1861 gagcctgccc tgagcgcagc cgaagggacg cagagggcgc gcagagattg ttgtggtgtt
     1921 ctaagcagaa ggaccagggt gcagaatgcc gtactctggt ccttcttgct gagaacacct
     1981 gccttgtctt accgtgtcct ttgcgctctt tgcgcctttg cggtttcttc gtacagtcta
     2041 gtcgtagtcc gcaggcaaac tggggtcacg gtagtgctca tagaagcggt gtagctgctt
     2101 agccgtcacc cgccccaccg aaagcgtcgg caggtcgtcc agcgggaaga aaccgacgcc
     2161 atccgtctcg ttgctagtgg ttggtgcgcc gccggtgatc tcgcacagga agaagacctt
     2221 gtagatgtgg aaggcgtgcg gcgggtgctc gtgccgcagc ctgtcgtaga gggccatcag
     2281 cttcacgatg cgggcctcgt agcccgactc ctccctgatt tcgcgcaacg tggccccggc
     2341 gggcgagtcg ccaatgtcgg cccagccgcc gggcacagtc caccgcccgt cggctatctc
     2401 tttcaccagc agcacacggt cgtcctggaa taccacgccg cgcacgtcaa ccttgggggt
     2461 ggcgtgccct gtctcgcgcg cgaaaatgcc gcgcaccttc tccgcgctca ccggctcgtg
     2521 cgaagccacg atttcggcgg cgatatcgag gagcgcgttg tagcggtcgc ggtcgtacgg
     2581 gtcttgggcg taggtgaggc cgttctgcgc gatagcctgc aatcgcctga cccactcaag
     2641 ccacttcggg tccatcacgg ctaccctgcc tcccctgccg gtgcgcccgg cctggctacc
     2701 actgccagcg tcacagtggc cttggctacc aggcgctcct tttcgcccga cctggcgaag
     2761 acctccgact ctaccacgat caacatccgc cccgccttga gcacctccgc ccggcacttc
     2821 agcgtctcac cccgcgcggt gttcagcagg tttatcttga actcggcgct cagcacaagc
     2881 tcggtgtccc ggatcaacgt ggtagccgcc gctcccgccg tgtggtcggc tatggtggcc
     2941 tgcacgcccg cgtggacgaa atcgtggtgc tggtagtgcc ggggtgacac cgccagcacg
     3001 ctctcgcacc agccggggcc gcactcggcc agccttatgc ccacgtcgtt gacgaacgcc
     3061 gcccgctcaa atatctcccg cacggcggat ttgtaattgg ggttggttgc ttccatcatt
     3121 gattaggacc tattcggcag ggtgtaccgg ccacacggca ggttgccggt caatcgtcta
     3181 gctcgatctc gccctgtccc atgtagacca cctggccgcc tacttgcacg cgcgttattt
     3241 cgccgccttc gcgcgtaatc tggatgtgaa tgaagctggg gcggcccatc tccacgcctt
     3301 gctcgcttac gatataagcg gtggggtcgg cgggcacgag gccgtgatgc accatgtagg
     3361 agccgagggg gccgcttgcc ccgccggtgg ccgggtcttc cccgatgccg aagtcagggg
     3421 cgaacatgcg actgtgcacg gtcgagcctg cgtactccac ctccggcgcg aagatgaaca
     3481 cctcgcccac gccaggcggc ccaagcaggc gggaggacac ttcctgccgc agcctcaggc
     3541 gtcgcatcgc cgccaggtcg cgcacaggca caatcaggaa gggcaccccg cacgacacca
     3601 cctccaccgg cagatcggtg ttgaggtcgc ccggcgcgat ggacagcatc tcggccactg
     3661 cggctacgtc ctcaatcacc gggccgaatc ggggaggtag ttgcgtcatc cagatcatgt
     3721 cgggcgcgcc gtccctgaac tcgaggcgca ccttgataag gccgacgcct tcctccagcc
     3781 gtaactctgc ctcgtccccc gaccggtcga acatgcgctc gcgggcgatt acgaacgagg
     3841 cgcccacggg ggggtggccc gccagcggca gctccttgcc gggcgtgaat atgcgcaggg
     3901 tgaagtggtt cgcgggg
//
LOCUS       DASRWZ010000261         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_570138, whole genome shotgun sequence.
ACCESSION   DASRWZ010000261 DASRWZ010000000
VERSION     DASRWZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570138"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1720
                     /locus_tag="VFH60_04050"
     CDS             <1..1720
                     /locus_tag="VFH60_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET6312985.1"
                     /translation="EQQAADRPHHHVPTGPGDSWGGQELKRVLTAASAWLARNAEALN
                     SLNVFPVPDGDTGTNMALTMQAAIKQVASSPSHSAAEIIAGISHGALMGARGNSGVIL
                     SQIWRGFADGIGGKERISARDFADGLTAATTTAYRAVLKPVEGTILTVMRETADGAKA
                     AASTLNSYSYVLSETVKASKASVARTPTLLAKLREAGVVDAGGQGLYVLLDGIRRYAE
                     GEDLETSAVEGTSGPAAEMEMGHAHVEHGEYGYCTNFLLIGHGWDFDEVRKRIASYGD
                     SAVIVGDDRVIKVHIHTEMPGTVLDYACALGSLRQIGITNMQDQHEEFLEMHADGSGG
                     APAMSDTAGPGDDAGTGVAQGRIAVLAVAAGPGLVKTFRSMGATAIIEGGQTMNPSTE
                     DILKLVESVPQDEVILLPNNGNIIMSARQTQSLTKKRVMVVPTDTIPQGMSALIAFNY
                     EASLEANAKAMEAAASQVETAEITRAVRDATVNGIAVKAGEVIGLLNNTLVATGKECE
                     EVAWDLLERMGASDRELITIYWGGDLAEGEAEAFCEQVRERYSGAEVELVHGGQPFYD
                     YIISAE"
     gene            1735..>2107
                     /locus_tag="VFH60_04055"
     CDS             1735..>2107
                     /locus_tag="VFH60_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET6312986.1"
                     /translation="MPRIRVVTDSACDLPDALLKQLHITVVPHSLGWAGDPEADRAHL
                     TAEQLHRRLRAEASTPEVHPPTIEEFTHVYRSLRETCDGVLSLHVSSKLSDTLANAAT
                     AREAFGPVGQGGPFPISLVDSL"
BASE COUNT          423 a          632 c          698 g          354 t
ORIGIN      
        1 agagcagcag gcagcagata gacctcacca ccatgtgccc accgggcctg gcgactcctg
       61 gggtgggcag gagttgaagc gcgttcttac ggcggcctcg gcatggctcg cccgcaacgc
      121 cgaggctctc aactccctca acgtcttccc cgtgccggat ggagatacag gcaccaacat
      181 ggccctgacc atgcaggccg ctatcaagca ggtggcaagc tcgcccagcc attccgcagc
      241 cgagataatc gcgggcatat cgcacggcgc gctcatgggc gcacgcggca actcaggcgt
      301 gatactctcc cagatttgga gaggcttcgc ggacggcatc ggcggcaagg aacgcatctc
      361 ggcccgcgat ttcgccgacg gcctcaccgc cgcaaccacc accgcctacc gcgccgtgct
      421 caagcccgta gagggcacta ttttgacggt catgcgggag acggctgatg gcgcgaaggc
      481 tgcggcttcc actctcaact catacagcta cgtgttgagc gagaccgtga aagcctccaa
      541 agcctcagtc gcccgcacgc ccacacttct agccaagctg agagaggcgg gcgtggttga
      601 cgcgggcggg caggggctat acgttttgct tgacggcata cggcgctacg ctgaggggga
      661 agacctggag acgagcgcgg ttgaggggac ttccggcccg gctgccgaga tggagatggg
      721 ccacgctcac gtagagcacg gcgagtacgg ctattgcacg aacttcctgc tgattgggca
      781 cgggtgggac tttgatgaag tgcggaagcg tatcgcctcc tacggtgatt cggctgtcat
      841 cgtgggtgat gaccgcgtga taaaagtgca tatccacacg gagatgcccg gcaccgtgct
      901 cgactacgcc tgcgcgcttg gtagcctgcg gcaaataggc atcaccaaca tgcaggacca
      961 gcatgaagag ttccttgaga tgcacgccga cggtagcggt ggcgcgcccg cgatgagcga
     1021 tacagcgggg ccgggagatg atgcaggcac gggagtcgcc cagggccgca tcgctgtgct
     1081 ggcggtggcg gcggggccgg ggttggtgaa gactttccgc agcatgggcg cgaccgccat
     1141 catcgagggc ggccagacga tgaaccccag cacggaagat attttgaaac tggtagagag
     1201 cgtgccccag gacgaagtga tactcctgcc caacaacggc aacatcatca tgtcggccag
     1261 gcagacccag agcctcacga agaagcgtgt gatggtggtg cctaccgaca cgatcccaca
     1321 gggtatgtcg gcgctgatag ccttcaacta cgaagccagc cttgaggcta acgctaaggc
     1381 tatggaagcc gccgcctcgc aggtggagac cgccgagatt acgcgggccg tgcgcgacgc
     1441 caccgtgaac ggtattgcgg tgaaggcagg cgaggtaatc ggcctgctca ataacaccct
     1501 ggtggcgacg ggtaaggagt gcgaggaggt tgcctgggac ctgctggagc gcatgggcgc
     1561 gagcgaccgc gagctaatca ccatctactg gggtggcgat ctcgcggagg gtgaggccga
     1621 ggcgttctgc gagcaggtgc gggagcgata ttcgggcgcg gaagtagagt tggtgcatgg
     1681 cggccaaccc ttctacgatt acatcatctc ggcagagtag cgtcagcaga gactatgccc
     1741 cgtattagag tagtaaccga cagcgcatgc gatttgccgg atgccctcct gaagcaactc
     1801 catattaccg tcgtgccgca ctccctgggt tgggcgggcg atccggaggc ggatagggcg
     1861 cacctcacgg cggagcagct acacaggcgt ctgcgcgccg aagcctcaac ccccgaagta
     1921 catcccccca ccattgagga gtttacgcac gtctaccgct cgttgcgcga gacctgcgat
     1981 ggcgtgctat cgctgcatgt gtcgtccaag ctgagcgata cgctggcgaa tgccgccaca
     2041 gcgcgagagg cgttcgggcc ggtgggccag ggcgggcctt tccccatctc cctggtagac
     2101 tcccttt
//
LOCUS       DASRWZ010000262         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_90238,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000262 DASRWZ010000000
VERSION     DASRWZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90238"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VFH60_04060"
     CDS             complement(<1..913)
                     /locus_tag="VFH60_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6312987.1"
                     /translation="MANDNPTFGWVMQPALFHTPDGVDSRDVSLARDMIAANERHIQV
                     AREGGFDTIWVEDHMGWGEKAHLECFTNLAWLAGRHPGLTYGTMVCGQAFRNPALLSK
                     MATNMFLLTQGKFILGIGAGNNPGEHHEFGYQFAPPAERIEQTEEAIKIIKALWNDSP
                     ATFHGKHYRIDNAYSSPRPDEWIPLCIGGMGEKKLLRLVAEYGDWWCADLAPVDVFAH
                     KASVLARHCADLGRDPADIVHGQVTWVSVEEDSAHATRWGHLHIVAGNPDEVTRELEA
                     YRDAGADHFMVRFMDYPRTDNLERFINK"
     gene            1241..2311
                     /locus_tag="VFH60_04065"
     CDS             1241..2311
                     /locus_tag="VFH60_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET6312988.1"
                     /translation="MRYLGNKDSLLPFIDEVLAMHGVKSTRDKPLCVCDPFTGTTSVA
                     RHLKRQEWQTVTGDLMTYSYALQHAYIGLNEAPVFANLVNAGVLDGSVNLPVPLHRVI
                     AHLNNLRGVEGFFYWNYSPDGPEGRRYFTSGNALRIDAIRNTINWWWERSWLTESERF
                     LLTAALIEAVSRVANIAGTYAAYLKRWDSRARKPLMLAVPQMVHSVYEHRINLADAND
                     MVPNTECDLLYVDPPYNTRQYCTNYHLLETIAVGDEPETRGIAGLRNDGDGKKSAYSK
                     AGTAEAALSKLVADSKAKWVLVSYNSEGVIPHDKMVDILSQRGNVEIYACEYRRFRSD
                     ADGENRQYKPNNTVQEMLYWVG"
BASE COUNT          537 a          743 c          853 g          502 t
ORIGIN      
        1 ctttgttgat gaagcgttcc aggttatcgg tgcgtgggta gtccatgaag cggaccatga
       61 agtgatcggc accggcgtcg cggtaagctt ccagttcgcg ggtgacttcg tcggggttgc
      121 ccgctactat gtgcaggtgg ccccagcggg tagcgtgagc cgagtcctct tccacgctga
      181 cccaggtgac ctggccgtgc acgatatcgg cggggtcgcg gcccaggtcg gcgcagtgcc
      241 gggcgagcac ggacgctttg tgcgcgaaca cgtccaccgg ggcgagatcg gcgcaccacc
      301 aatcgccgta ctctgctacc agcctgagca gcttcttctc gcccatgccg ccgatgcaga
      361 gcggtatcca ctcgtcgggg cggggtgagc tataggcgtt gtctatgcgg tagtgcttgc
      421 cgtggaaggt ggcggggctg tcgttccaca gggccttgat tatcttgatg gcctcctcgg
      481 tctgctcgat gcgctcggcg ggaggggcga actggtagcc gaactcgtgg tgctcgccgg
      541 ggttgttgcc cgcgcctatg cccaggatga acttgccctg ggtgagcagg aacatattgg
      601 tcgccatctt ggagagaagc gcggggttgc ggaaggcctg cccgcacacc atcgtgccat
      661 aggtgagacc ggggtgtcgc cccgccagcc aggccaagtt ggtgaagcat tcgaggtggg
      721 ccttctcgcc ccagcccatg tggtcttcca cccagatggt gtcgaagcca ccttcgcggg
      781 cgacctggat gtgccgctcg ttggcggcta tcatgtcgcg agcgaggctg acgtcgcggg
      841 agtccacccc atcgggcgta tggaacaagg ccggctgcat cacccagccg aacgtcgggt
      901 tgtcattagc caaaataatt cctcctaaga tatcggtgcg ggggtctaca gcagcaagca
      961 gggtgccaac ggagtaatga gtgatgagga ctgagggctg agtaggagac gaaaacgcaa
     1021 agacgcaaag gacgcaaagg acacgcaaag aagattaagg ttaggacgaa tcctatttcg
     1081 gcgtttgtac ttggtactct gacgtaccct tgcatctagc tatagtgtgg tgcataatag
     1141 cgaaacgacg tgcaggatga gcgcaatggc gtagctcaat cctcagtcct cagtcctcat
     1201 cactcattac tcctcgttac tcgtcagaaa ggccgcctcg atgcgctacc tgggtaacaa
     1261 agacagcttg ctgcccttca tcgacgaagt gcttgctatg cacggcgtga agagcacgcg
     1321 ggacaaaccg ctctgcgtgt gcgacccttt cacaggcacc acctcggtgg cgcgccacct
     1381 caaacggcag gagtggcaga cggtcacggg cgacctgatg acctattcgt acgccctgca
     1441 acacgcctac atcggcctca acgaagcccc ggtcttcgcc aacctggtga acgcgggggt
     1501 gctcgacggc agcgtcaacc tcccggtgcc cctccatcgc gtcatagcgc acctgaacaa
     1561 cctgcgcggc gtggaggggt tcttttattg gaactactcg cccgacgggc cggagggcag
     1621 gcgctatttc acctcgggca acgccctgcg catcgacgct atcaggaaca ccatcaactg
     1681 gtggtgggag cggagctggc tgaccgagtc ggagcggttt ttgctcacgg cggctctgat
     1741 agaggcggtg tcgagggtag ccaatatcgc gggcacgtac gcggcctacc tgaagcggtg
     1801 ggactcgcgg gcgcgcaagc cactgatgct ggcggtgccc cagatggtgc atagcgtcta
     1861 cgagcaccgg atcaacctgg ccgacgcgaa cgacatggtg cccaacacgg agtgcgacct
     1921 gctgtacgtc gatccgccct acaacacccg gcagtactgc accaattacc acctgctcga
     1981 gacgatagcg gtgggggacg agccggagac gaggggcatc gcggggctgc gcaacgacgg
     2041 cgacggcaag aagtcggcct actccaaggc ggggaccgcc gaagctgccc tgtcgaagct
     2101 ggtggcagac tcgaaggcga agtgggtgct ggtaagctac aacagcgagg gtgttatccc
     2161 gcacgacaag atggtggaca tactgtcgca gcggggcaac gtggagatat acgcgtgcga
     2221 gtaccgccgc ttccgctccg acgccgacgg cgaaaaccgc cagtacaagc cgaacaatac
     2281 ggtgcaggag atgctgtact gggtagggta gggatgaatt cttaattttt aattcttaat
     2341 tggattaggt aatactacat ctattacgta cttggaggcg gggatgccgc aacgcaaggt
     2401 tgaggggtta cagggcggca gtagggaagc gaagccaatt aagaattcaa aattaagaat
     2461 taagaattcg ccgcccgcgg cgtcgcaccc gcgtaacagg ttgaacaatt tgacggggag
     2521 tgagtgggtg catttcttgg cgtcggtgga ggctacggct tttcctacta cggggccgca
     2581 gtcttacggg cacaatttgc ggcgggtgca cccctcgcct aagccgcccc agttg
//
LOCUS       DASRWZ010000263         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_912849, whole genome shotgun sequence.
ACCESSION   DASRWZ010000263 DASRWZ010000000
VERSION     DASRWZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912849"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFH60_04070"
     CDS             <1..612
                     /locus_tag="VFH60_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312989.1"
                     /translation="ISKSIGARAIILAAATLSMLPLAACNQAAPDGATPTRAQVRPTK
                     TPGDKMQPPADFSVQYEWRAGSMPPPYHYEYTIRIGPGSEGQIVYLPDYQSEGVPTWT
                     ETITVTSRQLGDLYALVVDRKLLREKWGRVQDPPVGGSVQWAEITAGGKTYKVPTQLQ
                     GFQESAAASLYEAIKALVPQATWDKLEAQREQYVKDYQAKDGD"
     gene            670..>2030
                     /locus_tag="VFH60_04075"
     CDS             670..>2030
                     /locus_tag="VFH60_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6312990.1"
                     /translation="MHKAARAKAVARIVAAVALLLASGSLLAGGSLASAGMPVRASTE
                     ASTQAGAAQPTGKIAPDVLSATTGGQVAPIAILLADQADLSAAYGITDRDARGRFVYD
                     TLRSHANRTQAALRAELDALGTRYTSFWVANVIFTQAGRPLVERLAARGDVKLIESNA
                     SRRVIADPMLPGQADGPLSPQAVEWNIARVHAPQVWSLGYRGEGIVIGIHDTGVQWDH
                     PALKPQYRGWNGVTADHNYNWHDAIDVDAGTCPGNSPVPCEGYSHGSHVAGIAVGDDG
                     GGNQIGVAPGADWIACRSMDTAGDGSPETYTECLQFFLAPYDLAGQNANPSLRPHVVN
                     NSWGCTPSEGCDSANLQVLKPIVENVQAAGILVVSSSGNSGPTCGSVNQPIAIYDAAF
                     ATGGTNINDELWSGSSRGPVTVDGSNRLKPNISAPGQNVRSSLKGGGYGLLTGTSMAS
                     PHVA"
BASE COUNT          407 a          702 c          640 g          281 t
ORIGIN      
        1 atctcgaagt ccataggggc cagggctatc atactggctg cggccactct gagcatgctt
       61 ccactggccg cctgcaacca ggcagcaccc gatggcgcta cgcccacccg cgcacaggtc
      121 aggcccacca agaccccagg agacaaaatg cagccgcccg ccgacttcag cgttcagtac
      181 gaatggcggg ccggtagcat gccacctcca taccactacg agtacactat tcgcatcggc
      241 cccggctcgg aaggtcagat cgtctacctc cccgattacc aatccgaggg cgtccctacc
      301 tggaccgaaa ccatcaccgt cacgagccgg cagcttggcg acctgtatgc cctggttgtt
      361 gaccgcaagc tgctccggga gaaatggggc agagtgcagg acccacccgt cggcgggagc
      421 gtgcaatggg cggagattac cgcgggcggc aagacgtaca aggtcccaac ccagttgcaa
      481 ggcttccagg agagcgccgc cgcgagcctg tacgaagcga tcaaggccct cgtaccccag
      541 gctacctggg acaaactgga agcccagcgc gagcagtacg ttaaagacta ccaggcgaag
      601 gacggggact gaccgggcag ggcagccacc cgcacaatgt cggtgtaaac gcaaaggaag
      661 gagcacaaat tgcacaaggc cgcacgcgcc aaagcggtcg ccaggattgt agcggctgtc
      721 gccttgttgc tggcgtcggg gtcgctgctc gcgggggggt ctctcgcctc ggcgggcatg
      781 ccggtgcgcg cctccacgga ggcaagcacc caggccggcg cagcccagcc caccggtaag
      841 atcgcgccgg acgtgctgag cgccaccacc ggcgggcaag tagcgcccat cgccatcctg
      901 ctggccgacc aggcagacct gagcgccgcc tacggcatca ccgacaggga cgcccgcggc
      961 cgcttcgtct acgacaccct caggtcgcac gccaacagga cccaggcggc cctgcgcgcg
     1021 gagcttgacg cgctcggcac ccgctatacc tccttctggg tcgccaacgt tattttcacc
     1081 caggccggcc gccccctggt ggagagactg gcggcccgcg gcgacgtaaa gctgatcgag
     1141 tcgaacgcca gcaggcgcgt gattgccgac cccatgctac cgggccaggc ggatggtccc
     1201 ctcagtcccc aggccgtcga gtggaatata gcgcgagtgc acgcccccca agtatggagc
     1261 ctgggctaca ggggcgaagg catcgtcatc ggcatacacg acaccggcgt gcagtgggac
     1321 cacccggccc tcaagcccca gtaccgcggc tggaacggcg tcacggcgga ccacaattac
     1381 aactggcacg acgcgataga cgttgacgcc gggacttgcc ccggcaacag ccccgtgccc
     1441 tgcgaaggct acagccacgg tagccacgta gccggtatcg cggtgggcga cgacggcggc
     1501 ggtaaccaga taggcgtggc ccccggcgcg gattggatag cctgccgcag catggacacg
     1561 gccggtgacg gctccccaga aacgtacacc gagtgcctgc aattcttcct cgcgccctac
     1621 gacctcgcgg gccagaacgc caaccccagc ctgcgcccgc acgtggtcaa caatagctgg
     1681 ggctgcacgc cctccgaagg ctgcgatagc gccaacctcc aggtgttgaa gcccatagtg
     1741 gagaacgtgc aggcagcggg catactcgtg gtctcctcgt cgggcaacag cgggccgacc
     1801 tgcggtagcg tgaaccagcc catcgctatc tacgacgctg ctttcgccac gggaggcact
     1861 aatattaatg acgagctatg gagcggcagc agccgggggc cggtgaccgt ggatggctcg
     1921 aaccgcctca agccgaacat ctcagcgccg gggcagaacg tcaggtcgtc cctgaagggc
     1981 ggcggctacg ggctactgac cggcacctct atggcaagcc cccacgtcgc
//
LOCUS       DASRWZ010000264         7160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1118358, whole genome shotgun sequence.
ACCESSION   DASRWZ010000264 DASRWZ010000000
VERSION     DASRWZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118358"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1488)
                     /locus_tag="VFH60_04080"
     CDS             complement(13..1488)
                     /locus_tag="VFH60_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312991.1"
                     /translation="MILSTALQDSASFASDLSTIGQGVLDSLTPFFLPFLIFSLVATL
                     LSVSAQFGPWRTLAPFGLSLWAVIVACGGSIIAVSVLAGFLTLGLLLGAEPQSFSDFL
                     LFVLSCGVTTAVLGAASGVLQWLVFLPNLGHISAIRWVFGNVLFWSISYPLSLLLAAG
                     FSLFRRDDILDPMMVVFGIVYGCILGVKIGGLAAEHTPPESKGAAEWLVLAVAGRWLF
                     ALYLALLLLVVVAPIYGSVSAGMAYQHGVNAAEEQRSQVATALMVPVHATIAAEEKER
                     AIQADLTAVALAAIPTPLLVLAPVSTDVATFAEVTPHLENYVTRLRQEGFEFCGSKLN
                     LKKRNTYVCVKHEPHDGTSWDLRPAIMEGEEPMEVFISKPDPTISKGDNTIVVSVLRT
                     DVNAYTTFNFRGLQGQLPAPHTHEHVLGEPSDRFPFMGVYLDGHVLPCARLVGRFDLL
                     QLDYDQSTGEIHSMAVDFVQYCADGSSSIFGAIRYKSTVPL"
     gene            complement(1679..4228)
                     /locus_tag="VFH60_04085"
     CDS             complement(1679..4228)
                     /locus_tag="VFH60_04085"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6312992.1"
                     /translation="MDSSRPGEATETTPVEPVEPVEPVVVTVERDNSDRSSERFRTLL
                     GVMIAIVTVIGAVVAWRSALAGTEAGNADDAGIIAALNTQETNTLSDIISSQHRTSFL
                     EYWQHKQQIEELAADGSLENIPEGDPNGVIRQVTEAADLATASKLFFPARYLNPDGTY
                     NVTKERAETLAEEAQRRDMNAQQHFDDANQWRDKSLALVVSLVVLGIALWLFALAETI
                     EHRVKYALALGGLGFLLLGAGAALAVETNVSLVDVYSVGTMLALVMAVLVVLGVVAIL
                     VMRRGTGDSMTASSDDESPREARFKQAATVLIATVALLGALAAYLQADASNRGDTAIR
                     RSQLQAAEALGVQSTGQANFNFHYGGAAQAWEELKVLANAATEVGDAKAAARYTAIQT
                     DLANLSELLRPPYFDAATSAAPDTAAYEADQYVAKAETLAESSTLGGNLENAWEEKSN
                     SYIIHLTLLATALALLGLSLTLSGRVRPLFVGVGSAITVVTVLWMGLVFAKPVPEIPQ
                     AAVEAYGRGVGAAYKSDYKAAIAAFDEAVAAAPGYSTALYERGAARGADGDLEGAIKD
                     YEAAQAAGKDDAALAWDLGLSYYQMGRFDDAIRAHRAALERDPRRVYVQLDLAVALLA
                     SGRTEEAKAEYQKAKDSISSHVAAAKAAGSEPPPSLLYYLDLGADDLEGLFYQLNDQP
                     RPWASAPPKDKIVASEGMLQVVEEQFFELKSLAVALEYTGKPPAGPVEAEVEPLQFGL
                     QGEGDAFDIADTFASETKDVWIIYKFNGMQDGQQVVWKVYVDGVEYPEYRTVSIWEEG
                     PSGEASQPLTDDFAYGSTYSFDPGEYTVEMYVDSQLTQRGFFVVEEAVEED"
     gene            complement(4353..5000)
                     /locus_tag="VFH60_04090"
     CDS             complement(4353..5000)
                     /locus_tag="VFH60_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6312993.1"
                     /translation="MPIRAVLFDLDGTLINSIDHIVDCWQHTVRTGLGREISREEVLP
                     TIGRALVACLDEMAPGRGQELYGIYTNYERDRHDAAVHLIAGTGETLDALLAGGYKLG
                     VVTSKVLPVAMSGLRLFALDRYFEVIITQEDTERHKPYPDPLLVACERLTIPPVEAVY
                     VGDAHPDVEAGKAAGVLTVGVTWGAAGEAIRAAEPDCILSGMAELPGVLKLMSPA"
     gene            complement(5109..5195)
                     /locus_tag="VFH60_04095"
     tRNA            complement(5109..5195)
                     /locus_tag="VFH60_04095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5159..5161),aa:Ser,seq:gga)
     gene            complement(5289..5376)
                     /locus_tag="VFH60_04100"
     tRNA            complement(5289..5376)
                     /locus_tag="VFH60_04100"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5340..5342),aa:Ser,seq:cga)
     gene            complement(5414..5486)
                     /locus_tag="VFH60_04105"
     tRNA            complement(5414..5486)
                     /locus_tag="VFH60_04105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5451..5453),aa:Arg,seq:gcg)
     gene            complement(5557..6525)
                     /locus_tag="VFH60_04110"
     CDS             complement(5557..6525)
                     /locus_tag="VFH60_04110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6312994.1"
                     /translation="MPHLLPPDPPISPTNRVLSDVPDAYWGPIIAALRARLDPPLGRW
                     RRLPTGKNVVLELGDEAILKLIPPCWAEDAGREAAALRAVPVAGPVATPTLLASDELE
                     GWTVLLLRRLPGEVLSGRWPSLERPVRARLVAHLGEAAAWLHSVEVPAGSPLPYDWAA
                     HLRGDIEDAPRQLAQAGAPQALQASWPAFLRSVGPLPAPDSPFVLLHGDLSLGNVLVQ
                     ERAGELLMTGLLDFGDASLGDAVHDWLSPGVHNFAGDPAVLGAFFDGYGLPPSERTPA
                     LQAHLLARSLLYYGWGYLARKFPLQGATGWAEVAGVVWPIAATARG"
     gene            6688..6945
                     /locus_tag="VFH60_04115"
     CDS             6688..6945
                     /locus_tag="VFH60_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312995.1"
                     /translation="MTPIIEPDDDAPVGRVLSRREVLGLLAGLGGVTLLAACDPSQPG
                     AAQPTSAAATSGAATQTAPAQAAATASGTPLNAVVFLGGAR"
BASE COUNT         1425 a         2323 c         2115 g         1297 t
ORIGIN      
        1 gaagaggact tactaaaggg gcacagtcga cttataacgg atggcgccaa atatcgagga
       61 ggaaccgtcg gcacagtact gaacaaagtc cacggccatg ctgtgaattt cacctgtgct
      121 ctgatcgtag tctaattgga gaagatcaaa cctgccaacc agtcgcgcac agggcagaac
      181 atgcccatcc aggtaaacac ccataaatgg gaacctatct gatggctcac ccagcacatg
      241 ctcgtgagta tgcggagccg gtagctgacc ctgcaacccc ctgaagttaa aggttgtata
      301 tgcattcacg tcggtacgca acacgctcac cacgatggta ttgtccccct tcgagatcgt
      361 agggtccggc ttcgatataa agacctccat cggttcttct ccttccatga tggcgggtct
      421 aaggtcccag cttgttccat cgtgcggctc gtgtttaaca cacacgtagg tattacgttt
      481 cttcagattc aacttactcc cgcaaaactc aaatccttct tgtctcaggc gcgtaacata
      541 gttctcaagg tgtggcgtga cctcagcgaa cgtagctaca tccgtgctta ccggagcaag
      601 cacaagcagg ggcgtgggta tcgcggccag cgccactgct gtcaggtctg cctggatggc
      661 gcgctccttc tcctccgccg ctattgtagc gtgcacaggt accatcagcg ccgttgccac
      721 ctgggaccgc tgttcctctg ctgcgttcac gccgtgctgg taggccatgc cggcagatac
      781 gctaccatat atcggtgcaa caacgaccaa taacaataag gcgaggtaaa gggcaaagag
      841 ccaccgcccc gcgacggcaa gcacgagcca ctcagccgcg cccttgctct ccggcggcgt
      901 atgctcggca gccaggcccc cgatcttcac acccaatatg cacccataca cgatgccgaa
      961 cacgaccatc atggggtcga ggatgtcgtc gcgcctaaac agcgagaacc cggcggcaag
     1021 cagcaagctc agcgggtaag agatcgacca gaacagcaca ttaccgaaaa cccaccggat
     1081 cgcggaaatg tgccccaggt tggggaggaa gaccaaccac tgcaacacgc ccgaagccgc
     1141 ccccaggacg gctgtggtta ctccgcagct gaggacaaaa agcagaaagt cggagaagct
     1201 ctggggttca gcgcccaaca gcaggcccaa cgtaagaaaa ccggccagga cggacaccgc
     1261 tatgatgctc ccaccgcaag ccacgatgac tgcccacaaa gacaggccga acggcgcgag
     1321 cgtgcgccag gggccaaact gggcggatac ggaaagcagg gtggctacga gggagaagat
     1381 gagaaaggga agaaaaaacg gcgtgaggga gtcgagtacg ccctgcccga tagtggagag
     1441 gtcggaagca aaactagcgc tgtcctggag ggccgtcgat agaatcatga tagcgaaaag
     1501 tgcgatggca aaaattccgg catggctacc tgcactatac gcgcctcaca gccgtggaag
     1561 aatagcaact tggtactgta atcaattgcc atttgcccta tgttcaaaca aataaggaag
     1621 ccgccttcat tggcggcttc cctggatatg tgctccccac aagaggagtt acctgtcctc
     1681 agtcctcctc cacggcttct tcgaccacga agaagccgcg ctgcgtcagt tgcgaatcga
     1741 cgtacatctc gaccgtgtac tcgccggggt cgaagctgta ggtgctgccg tacgcgaagt
     1801 cgtccgtgag gggctggctg gcctcgccgg aggggccttc ttcccagatc gatacggtgc
     1861 ggtactcagg gtactccacg ccgtccacgt agaccttcca gaccacctgc tgcccgtcct
     1921 gcatgccgtt gaacttgtag atgatccaca cgtccttggt ctcggaggcg aacgtatcgg
     1981 ctatgtcgaa ggcgtcgccc tcgccctgca agccgaactg caacggctcg acttcagcct
     2041 ccacggggcc tgccgggggc ttacccgtgt attccagggc caccgccagg ctcttcagct
     2101 cgaagaactg ctcctcgacc acctgcaaca tgccctcgga cgccactatc ttgtccttgg
     2161 gcggcgcgct ggcccacggt cgcggctggt cgtttagctg gtagaacagg ccctccaggt
     2221 cgtccgcgcc caggtcgagg tagtacagga gagagggggg aggctcgctg cccgccgcct
     2281 tcgccgcggc tacatggctg ctgatgctgt ccttcgcctt ctggtactcc gccttcgcct
     2341 cctcggtcct accggaggca agcagggcca ccgcgaggtc tagctgcacg tagacgcgcc
     2401 tggggtcgcg ctccaacgcg gcgcggtgcg cccgtatcgc gtcgtcgaag cgacccatct
     2461 ggtagtagct gaggcccagg tcccacgcca gggcagcgtc gtccttgccc gccgcctgcg
     2521 ccgcttcgta gtccttgatg gccccttcca ggtcgccgtc tgccccccgc gccgcgccgc
     2581 gctcgtacag ggcagtggag taaccgggcg ccgccgccac ggcttcatcg aacgcggcta
     2641 tggctgcctt gtagtcagac ttgtacgccg cgcccactcc ccgcccgtac gcctcaacgg
     2701 ctgcctgcgg gatttcaggc acgggcttgg caaagaccaa ccccatccag aggaccgtca
     2761 ccaccgtgat ggctgagccg acgcccacga acagaggccg gacccgcccc gaaagcgtta
     2821 gcgacaggcc gaggagggcc agcgcggtgg cgagcagggt caggtggatg atgtagctgt
     2881 tcgacttctc ttcccaggcg ttctcaaggt tgccgcccag cgtggaggac tcggccaggg
     2941 tctcggcctt tgccacgtac tggtcggcct cgtaagccgc agtatccggg gccgcgctgg
     3001 tggccgcgtc gaagtagggc gggcgcaata gctcgctcaa gttggcgaga tctgtctgta
     3061 tagcggtgta gcgggcggcg gccttcgcgt cgcccacctc cgttgcggcg ttggcgagca
     3121 ccttcagctc ttcccacgcc tgggctgccc cgccgtagtg gaagttgaag ttggcctggc
     3181 cggtgctctg cactcccagg gcttccgccg cttgcagttg cgacctcctg atagcggtat
     3241 cgccccggtt gctcgcgtcg gcctgcaagt acgcggcgag cgctcccagc agggccaccg
     3301 tggcaataag caccgtcgcg gcctgcttga accgggcctc gcgcggggac tcgtcgtcgg
     3361 aagaagctgt catagagtcg ccggtgcccc ggcgcatcac cagaatcgcc acgacaccga
     3421 gcaccaccag cacggccatc accagggcga gcatcgtgcc caccgagtac acgtcaacaa
     3481 gcgacacgtt cgtctctacc gcgagagcgg cacccgcgcc caggaggagg aagccgaggc
     3541 cgcccagggc cagcgcgtac ttgacccggt gctctattgt ctcggccagc gcgaagagcc
     3601 acaacgcgat gcccagcacg accaggctga ccaccagcgc gagggacttg tctcgccact
     3661 ggttagcgtc atcgaagtgc tgctgcgcgt tcatgtctct gcgctgcgcc tcttcggcca
     3721 gggtctcggc gcgctctttg gtgacgttgt aggtgccatc ggggttgagg tagcgcgcgg
     3781 ggaagaacag cttgctggcc gtcgccaggt cggccgcctc ggtgacctgg cgaatgacgc
     3841 cgttagggtc accttcgggg atgttctcaa gggagccatc cgctgccagc tcctcgattt
     3901 gctgcttgtg ctgccagtac tccaggaagc tggtacggtg ctggctggat ataatatcgc
     3961 tcagcgtgtt ggtctcttga gtgttgaggg cggcgataat accggcgtcg tcggcgttgc
     4021 ccgcttcagt gcccgccagt gcggaccgcc aggcgacgac cgcgccaatc accgtcacaa
     4081 tcgctatcat cacgcccaac aaggtgcgga accgctcgct agacctgtca ctgttgtcgc
     4141 gctctaccgt gactaccaca ggctccacag gctccacagg ctccacaggt gtcgtttcgg
     4201 ttgcctcgcc cggccgagaa ctatccacta tcgtaacctc catgcaaaat ggtgaacagc
     4261 gccaaaacag gcaccgatta agtacggccc catgcactgc tcctattata aagccaagca
     4321 gccacttaag actacgtttt caacatggat tctcaagcag gcgacattag cttcaacaca
     4381 ccgggaagct cggccatgcc ggacaggatg cagtccggct cggccgcgcg gatagcctca
     4441 ccggcagctc cccacgtaac ccccaccgtg agcactcccg ccgccttgcc cgcttctacg
     4501 tcggggtggg catccccgac gtagacggcc tcgacagggg gtatcgttag acgctcgcag
     4561 gcgacaagca gggggtcggg gtaaggcttg tgccgctcgg tgtcctcttg cgtgataatc
     4621 acctcaaagt agcggtccag ggcgaagagg cgcaggccgc tcatcgccac gggcagcacc
     4681 tttgaagtga ccacccccag tttgtaaccc cctgccagca gcgcgtcgag cgtctcgccc
     4741 gtgcccgcaa tgaggtgcac agcggcgtcg tggcggtccc gctcgtagtt ggtgtatatg
     4801 ccgtacagtt cctgccctcg ccctggcgcc atctcatcga ggcaggctac gagggcgcgg
     4861 ccaatggtgg gcagcacctc ctcccggctt atctcccgcc ccaggccggt acgcaccgtg
     4921 tgctgccagc agtccacaat atggtctatc gagttgatga gggtaccatc gaggtcaaac
     4981 aaaacggcac gaattggcat ggtatatcct ttaggaacga tggatgataa caatgctacc
     5041 caactcagca ctcataactc ataactccta actaaaaaag acgccccaca aggaagcgtc
     5101 ttttacggcg gagagggtgg gattcgaacc cacgaggagc ttgcacccct acccgctttc
     5161 caggcgagcg ccctaggcct ctaggcgacc tctccagatt ttagattttg ggttttggat
     5221 tttagattgg agacttggat aggcagcccg gaacagcaat tcaaaatcta aaacctaaaa
     5281 tcgaccagcg gagagggtgg gattcgaacc cacggaaggc tcagcacctt cagtcgtttt
     5341 cgagacgacc gcactcggcc actatgcgac ctctccgaga caaaaaaaga ccggccaggg
     5401 ggccggccta atgcgtgact ggagggactc gaacccccgg cctttgggtc cgcaacccaa
     5461 cgctctatcc actgagctac agccacccgg ttcgctttac agccttacga ttataacaaa
     5521 ctccattcga gattgcaagc tagtcgctct cgcgcctcac ccacgcgccg tagcagcaat
     5581 gggccatacg acacccgcta cctcggccca gcctgtcgcc ccttgcaacg ggaacttacg
     5641 cgccaggtaa ccccagccgt agtagaggag gctccgggcc agcaggtgag cttgcagcgc
     5701 gggagtgcgc tctgaaggag gcaggccgta gccgtcgaag aacgccccca gcaccgccgg
     5761 gtcgcccgcg aagttgtgca ctccgggcga gagccagtca tggacggcat cacccagcga
     5821 agcgtccccg aagtcgagca ggcccgtcat tagcaactcc ccggcacgct cttgcacgag
     5881 cacgttgccc aggctcaggt cgccatgcag caacacgaag gggctgtctg gggcgggcaa
     5941 ggggcctacg gagcgcagga acgcgggcca gctagcctgt agagcctggg gagcgccagc
     6001 ctgggccaac tgccggggcg cgtcctctat atccccgcgt aggtgtgctg cccagtcgta
     6061 ggggagaggc gacccggcgg gcacctccac gctgtgcagc caggccgccg cctcacctag
     6121 gtgcgcgaca aggcgcgcac gcaccggccg ctccaatgac ggccaccgtc ctgacaggac
     6181 ctcgcccggc aacctgcgca gcagtagcac cgtccaccct tccagttcgt ccgatgccag
     6241 cagagtaggc gttgctaccg gccccgcgac aggcaccgct cgcagggccg ccgcctctcg
     6301 ccccgcgtcc tcggcccagc agggcgggat gagcttgagg atagcttcat cgcccaactc
     6361 gaggaccacg ttcttgccgg tgggcaggcg tcgccaacga cccagaggcg ggtccagccg
     6421 ggcgcgcaac gccgcgatga ttggccccca gtaggcgtca ggaacgtcgc tcagcacgcg
     6481 gttggtgggg cttataggtg ggtcaggtgg caggaggtgt ggcacggtgc gtcggtcctt
     6541 tttcggggtt agggggcaaa ccttacttgg ccaaaaagcg tattatcttc cgtagtccgt
     6601 cttcaccaag tcttaattat tctcgcgatc tgtcttaatg cggcgagtgt acactggtct
     6661 ttggaaagaa caatcaagga gagacctgtg acacctatta tagaacctga cgatgatgcc
     6721 ccggtagggc gcgtcctctc ccgacgagag gtgctaggct tgttggccgg gttgggaggg
     6781 gtgacattgc ttgcggcttg cgacccctcc cagcccggcg cagcccaacc cacaagtgcc
     6841 gccgctacgt ccggtgcggc cacccagacg gccccggcgc aggcggccgc tacggccagt
     6901 ggcactcccc tgaacgcggt agtctttctc ggaggtgcgc gttagcccta cgtgacgccc
     6961 tgtggctaaa gccacgggct gagaagaggc aaagtcccct gcgggactac cctcacctta
     7021 cacagtctcc gaagtagact tcgctacttc ccagcccgtg gctttagcca cagggcgtcc
     7081 ccgcaggtcg tccccgctcc tctaacgccc actgtccact atccatcgtc cgcatgcatc
     7141 catgctactt gtatcacttt
//
LOCUS       DASRWZ010000265         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_21711,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000265 DASRWZ010000000
VERSION     DASRWZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21711"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            458..1885
                     /gene="ftsA"
                     /locus_tag="VFH60_04120"
     CDS             458..1885
                     /gene="ftsA"
                     /locus_tag="VFH60_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET6312996.1"
                     /translation="MPNNDRIIVGIDVGTNKICTIVAEADAGPGNRLNVLGVGAVASE
                     GIAKGMITDIHKASASIAHSLEAAERQSGCRILSAYVSISGNHISSMNSRGLVAVSHP
                     DRIIGQDDVTRAVDAARAMAIPAGRDVLHVIPRTYTVDGQDGIQNPIGLSGFRLDVET
                     HVVTGANNVRENLVRCVQKADVEVDDLVLSPLASAEAVLTEQEKNMGVVLVDIGHGTT
                     DIAIYADGSVWHSKVVPIGGWHLTNDLVIVFNIPYESAETLKLQYGMAIAPGSSRHAQ
                     REAKAAGLGNGRVPAKGSFAAVSANGSGHGAHGVPLPVDVLMATVAEEAGLDEGDTLE
                     AETFEGNLRRISRDELNEVMAARLDQLFGLVGDEIRRSGYEGLLPAGMVLTGGVAQMS
                     NIEALAAGKLRLPVRMGHPRRIGGLADVFDSPAYATGVGLILWGMKPGTVNLPVTEER
                     PAHPKGRGFNTGVLADWLNKFMPRG"
     gene            2212..>3019
                     /gene="ftsZ"
                     /locus_tag="VFH60_04125"
     CDS             2212..>3019
                     /gene="ftsZ"
                     /locus_tag="VFH60_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET6312997.1"
                     /translation="MNNNPNRPVHIQYGSGRDNGLPAENYAQIKVIGVGGGGSNAVNR
                     MIQAGVKGVDFIAVNTDAQALMHSEAPTRIRIGDKLTKGLGSGGNPDVGRRAADETSE
                     ELFEVLKGADMVFITAGMGGGTGTGASPVIAQIAREIGALTVGVVTKPFTFEGSRRRL
                     AAEEGLNGLKEQVDTLITIPNDRLLQIADKKTTLNEAFRIADDVLRQGIQGISDLITE
                     PGLINLDFADVKSIMQLAGSALMAIGRGTGDNRCIDAARQAIESPLLEISI"
BASE COUNT          630 a          885 c          987 g          517 t
ORIGIN      
        1 acgctcagga tgacaagggg gaatacaggc tgacagggga ggggcttggg ccgcccggtg
       61 tgacaagaag agtcaaccac aaggggcgag ggtgcatagg gctgttgcgt tcgacgtgga
      121 ctgatttcgg cgcacaggaa catgggtacc acttcttaga acacctgttt tgctgccggg
      181 gtctgccgcc tgcaacaagc gcaaacttat gggtacaacc agtagataca actaggactt
      241 atggaaaact ttccacagaa cggtggaaac cagagtataa tagggaccga cggtgtagaa
      301 tggtctcctc agtaaccaat gaggaggaga cgagataaag gtccttgggg ttgccggtca
      361 ggccctcgta atttcggcat tggctcaaca ccttatgttg ggctgcggct tgccgttgta
      421 attttgcccc gtggaccccg cctcgtcgcg cacgcacatg ccaaataacg acaggatcat
      481 cgtcggcata gatgtcggca ccaacaagat ctgcaccata gtggccgagg cagacgccgg
      541 gccgggtaac cgcctcaacg tgctaggggt gggcgcggtg gcctcggagg gtatcgccaa
      601 ggggatgatc accgacatac acaaggccag cgcctccata gcccactcgc tggaagccgc
      661 cgagcgccag tcggggtgca ggatactctc cgcttacgtg tcgatctccg gcaaccatat
      721 ctcctcgatg aacagccggg gcctcgtggc ggtgtcgcac cccgaccgca tcatcggcca
      781 ggatgacgtg acgcgggcgg tggacgcggc ccgtgccatg gcgataccgg cggggcgcga
      841 cgtactgcac gtcattccac gcacctacac ggtggacggg caggacggca tacagaaccc
      901 cattggcctg tcggggttcc ggctcgatgt ggagacgcac gtggtgacgg gcgcgaacaa
      961 cgtgcgggaa aaccttgtgc ggtgcgtgca gaaggccgac gtggaggtgg acgacctggt
     1021 gctctcgccc ctggcttccg ccgaggctgt gctcaccgag caagagaaga acatgggcgt
     1081 ggtgctggtc gatatagggc acggcaccac cgacatcgcg atatacgcgg acggctcggt
     1141 gtggcacagc aaggtggtcc ccataggcgg ctggcacctg accaacgacc tcgtgattgt
     1201 gttcaacata ccctacgagt cggcggagac cctcaagttg cagtacggca tggcgattgc
     1261 ccccggcagc agccgccacg cccagcgcga ggcgaaagct gcgggcctgg gcaacggcag
     1321 agtgcccgcg aagggtagtt tcgccgcagt ctctgctaac ggctcgggac acggcgcaca
     1381 tggcgtaccg ctgccggtgg acgtgttgat ggctaccgtg gccgaggaag cggggctgga
     1441 cgaaggggat acgctggaag ctgagacctt cgaggggaac ctgcggcgga tcagccggga
     1501 cgagctgaac gaggtgatgg cggcgcgtct cgaccagcta ttcgggctgg tgggtgacga
     1561 gatcaggcgc agcggctacg aggggctgct tccggcgggc atggtgctca ccgggggcgt
     1621 ggcccagatg tcgaatatcg aggcgctggc ggcgggcaag ctacggctgc cggtacgcat
     1681 ggggcacccc cggcgcatag ggggcctggc cgacgtgttc gacagcccgg cgtacgctac
     1741 aggcgtgggc ctgatactct ggggcatgaa gccgggcact gtaaatctgc ctgtaacgga
     1801 ggagcgaccg gcacatccga agggaagggg cttcaacact ggcgtattgg ctgattggct
     1861 aaacaagttc atgccaaggg gttgagcggt ccataagagg gcgattcatt gccaatttgc
     1921 gcaaaacggg ccgaataagc accgaaacac cttgaacttc gtgctctgat ggtgtattat
     1981 cagtccttag tcccagtctt tttgggctac ccgtggaata attaagctca tcctgccgtt
     2041 tctcaactcg gcgcatgcct ctacaagggt aagggatagg gtaattcaca gcgcaaactc
     2101 acgcccctgg caaggacgtg gcagcgtttg cgtagcacgc tccgctggtc cgcgcggatg
     2161 agcggacgaa agagcaaccg gggaagagga ggattggaca tgtttccgcc tatgaataat
     2221 aacccgaaca ggccggtgca tatacagtat ggttcggggc gcgacaacgg tctgcccgcc
     2281 gagaactacg cccagatcaa ggtgataggc gtgggtggtg gcggctccaa tgccgtcaat
     2341 cgcatgatcc aggcgggcgt gaagggtgtg gacttcatcg cggtcaacac cgacgcccag
     2401 gccctcatgc attccgaagc ccccacccgc atccgcatcg gtgacaagct tactaagggc
     2461 cttggctcag gcggcaaccc cgacgtgggc cgccgcgccg ccgacgagac gagcgaagaa
     2521 ctgttcgagg tgctcaaagg cgctgacatg gtgttcatca ccgccggtat gggcggcggc
     2581 acgggcacgg gcgcttcccc cgtaatcgcc cagattgccc gcgaaatcgg cgcgctcact
     2641 gtgggcgtgg tcaccaagcc cttcacgttc gaaggctccc gccgccgcct cgccgccgag
     2701 gagggtctga acggcctcaa agagcaggtt gacaccctca tcaccatacc caacgaccgc
     2761 ctcctccaga ttgccgacaa aaagaccacc ctcaatgagg ccttccgaat cgccgacgac
     2821 gtgctgcgcc agggcatcca gggtatttcc gatctcatca ccgagccggg cctcatcaac
     2881 ctcgacttcg cggacgtgaa gagcatcatg cagcttgccg gttcggccct catggctatc
     2941 ggccggggca caggggacaa ccgctgcatc gacgcggccc gccaggctat cgagtcgccc
     3001 ctgctggaaa tcagcatag
//
LOCUS       DASRWZ010000266         1908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_501666, whole genome shotgun sequence.
ACCESSION   DASRWZ010000266 DASRWZ010000000
VERSION     DASRWZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501666"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFH60_04130"
     CDS             complement(<1..623)
                     /locus_tag="VFH60_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6312998.1"
                     /translation="MSLMERDEYAARYGSTRGRRTGAEVRVLLLWLAWIPLTVVGAVV
                     GRQLGLVIDYLAEVGGVLYFGELVLRGVFVGAVLGGLQGLALWDYLHLRGWLIWTGAT
                     ALGWAGRDLALYYTGGPIVNTMFDWGGPSLPLIGGVTGLVGGLMLGIAQSLVVRHTRM
                     PIEQIPWLLACTGGAALGRMGAGFAMNFGSADSLVAVAGAEALGIAFL"
     gene            complement(702..>1908)
                     /locus_tag="VFH60_04135"
     CDS             complement(702..>1908)
                     /locus_tag="VFH60_04135"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6312999.1"
                     /translation="IEVYDPNKAQPSTPTPIPQEERTALVDDVWSTVEQSYIDPEFNG
                     VDWEGLREEYRRRVLDAPTAQDYYGAIVEMVGLLDDGRSTFTPPSKVPMPATGAEEAG
                     FANIGVTRVFDDNSLLVLYVYPNGPAAKLGIKRRDRITAINGRPVTRAQDDLALSGIV
                     GSRLTLTVVSPGGQPRDMELKREIAVGPAPIFARQLSEDPTLVYLAMPTLSVANVDQI
                     SLYLLNKMQRQFLMQDVPLKGLVLDLRQTTGSSQQTLSFVLGEVTTGNLGNFESRDAY
                     NNFDLDVPEGPLYKKYGGLPLVLLVDEGTRGDAEIMTAALQAEGRAVVLGKRTAGNTV
                     SNETYPYPDGSVLSIAQWRFFLPDGTNIDGRGVTPDVEVREDWTQYAEDSDPYIPAAV
                     SLLHSLSNP"
BASE COUNT          340 a          643 c          600 g          325 t
ORIGIN      
        1 aggaacgcta tacccagcgc ctctgccccg gctaccgcca cgaggctgtc ggccgagccg
       61 aagttcatcg cgaaccccgc tcccatgcgg cccaaggccg cgccgccggt gcaggccaac
      121 agccacggta tctgctcgat aggcatcctg gtgtggcgca ccaccagaga ctgtgcgata
      181 cccagcatca gaccgcccac cagcccggtc accccgccga ttagaggcaa agaggggccg
      241 ccccagtcga acatcgtgtt cacgataggg ccgccagtgt agtagagcgc caggtcgcgc
      301 ccggcccaac cgagcgccgt cgccccggtc cagatcagcc agccacggag gtgcaggtag
      361 tcccacagcg ccagcccctg caacccgccc aggaccgccc ccacgaacac cccacgcaac
      421 accagctcgc caaagtagag cacgccgcca acctcggcaa ggtagtcaat gaccaggcca
      481 agctggcggc ccacgaccgc gcctacaacc gtgaggggta tccacgccag ccagagtagc
      541 agcacccgga cctccgcgcc ggtgcgccgc cccctggttg acccataccg ggccgcgtat
      601 tcgtctcgtt ccatcaggct catagtgctg ccttgttctg tctggggcga gtattcccgc
      661 tgctgttgct gaagctcatg gaaaagtacc ggctaccggc gctaggggtt gctcagcgag
      721 tgcagcaggg agacagccgc cgggatgtaa gggtcgctgt cctcggcgta ctgtgtccag
      781 tcctcgcgca cttccacgtc gggcgtgacg ccccggccat cgatgttggt gccgtccggc
      841 aggaagaagc gccactgcgc tatgctgagc accgagccat cagggtacgg gtaggtctcg
      901 ttagatacgg tattgcccgc cgtgcgcttg cccagcacca cagccctgcc ctcggcctgc
      961 aaagccgccg tcattatctc ggcgtccccc cgcgtgccct cgtccaccag taggaccaac
     1021 ggaaggccgc cgtacttttt gtacagcggc ccctccggca cgtccaggtc gaagttgtta
     1081 taggcgtcgc gggattcgaa gttgcccagg ttgccggtgg tcacctctcc caacacgaag
     1141 ctcaaagtct gctgagaact gccggtagtc tggcgcaaat cgagcaccag ccctttgagg
     1201 ggcacgtcct gcatgaggaa ctgccgctgc atcttgttga gcaggtagag gcttatctgg
     1261 tccacgttgg ccacgctcaa ggtaggcatg gcgaggtaga ccagggtggg gtcttcgcta
     1321 agctggcggg caaagatagg ggccggaccg accgcgatct cccgctttag ctccatgtcg
     1381 cggggctggc cgccgggcga taccacggtg agcgtcagcc tcgaaccgac gatgcccgac
     1441 agcgcgaggt cgtcctgggc gcgggtgacg gggcggccgt tgatggctgt aatgcggtcg
     1501 cggcgcttga tacctagctt ggcggcgggg ccgttggggt agacgtagag caccagcagc
     1561 gaattgtcgt caaatacccg cgtgacgccg atgttggcaa agcccgcttc ttccgcaccc
     1621 gtcgcgggca taggcacctt gctgggggga gtgaaggtgg agcgcccgtc gtccaagagg
     1681 ccgaccatct ctacgatagc cccgtagtag tcctgcgcgg taggggcatc cagcacccgc
     1741 cgccggtact cttcccgcag cccctcccag tccacgccgt tgaactcagg atcgatgtag
     1801 ctctgctcga cagtgctcca cacgtcatcg accagggccg tgcgctcctc ctgggggatg
     1861 ggggtggggg tggaaggttg ggctttattg gggtcgtaca cctctatc
//
LOCUS       DASRWZ010000267         7430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_844726, whole genome shotgun sequence.
ACCESSION   DASRWZ010000267 DASRWZ010000000
VERSION     DASRWZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844726"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="VFH60_04140"
     CDS             <1..1328
                     /locus_tag="VFH60_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET6313000.1"
                     /translation="LEQGSTAEGSGHRDVVMRVLRAALEAADPHRAVRAALRGRDFSH
                     VAGDIYVLGAGKAGAAMSGAAEEVLGERIAGGLVVVKEGHSGDVRPGRIEVVEASHPV
                     PDERGVQATGRLLELAGAAGENDLVLCLISGGGSALMSAPAEGLTLEDLRQTTQSLLR
                     AGATINELNSARKHLSRVSGGRLARAASPARVLSLILSDVTGSPLDVIASGPTAPDPT
                     TFHDALAVLERYALNENVPAAVRDRLRAGAEGGVEETPKPDDPLFARVENVIVASNVI
                     AVEAGAAKAREMGLDAAIMSTYIEGEAAEVGLVLAGVAKEIAAYGRPVDRPGCVLFGG
                     ETTVTVRGNGTGGRNTELALAAAVGIREMGPGVLVASLATDGGDGTSPSAGAIIDGTT
                     IARGEALGLDHHAALANNDSYTYLSRVGDALMLGPTGTNVNDVMAVFVF"
     gene            1410..3191
                     /locus_tag="VFH60_04145"
     CDS             1410..3191
                     /locus_tag="VFH60_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HET6313001.1"
                     /translation="MKLGGFTFVLHSHMPFYRGAGKWPHGEESLHEVMAESYVPLVEA
                     LTDLLESGIQAKITLGVTPVLAEQLADPLIASNFEAYVQQEIESAQADVHRFEASGEA
                     HFLYLAHFYLDWYAHVLETFRNRFDRDIIGALRRLQDAGAIEIITSAATHPYLPLMQR
                     DSTIDAQIAIGKRASMRHFGREPRAIWLPECAYRPAYYATASDGTQYERPGLESWLQN
                     HGIGLFFSESSAVEGSRVLGRPEFTTTELFVERPRMVQAAASGSDIYGQVAQVVEEMG
                     YESAGFVTERRVVPFESGGQAWEQGGTFQAYYVRESEVAVVGRNRAVGEQVWAAKLGY
                     PGDAEYREFHKKDAEHGFQYWRVTDSKTDLAYKQPYDPYKAGQRIEAHADHFVSLLEE
                     QFRWYNVKSGGTPGIIASAYDTELFGHWWFEGVQFLKEVLTRLAKHERIDLVTTSEWL
                     EQRPPERVIDLPESSWGHAGTHVTWINPETSWMWDSVHAAESRMEELVERFESASGDV
                     EAALNQAARELVLLQASDWEFLYTTGQARQYATDRFMEHVDRFNALADAVEQDGERAA
                     SLAREYGERDNLFPDIDYRLFARKQPL"
     gene            complement(3413..3760)
                     /locus_tag="VFH60_04150"
     CDS             complement(3413..3760)
                     /locus_tag="VFH60_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313002.1"
                     /translation="MGNGSGGNNPELEIEDYGTSDSEGGSGSAFGSGGGQGSSGGGGG
                     ADSASGTGVERPGAQFGEFGETGTGTDLTVEMEGFDARTGGIKGGQEGADDAAGPGQY
                     GGETGGLGSSGRS"
     gene            complement(4071..4895)
                     /locus_tag="VFH60_04155"
     CDS             complement(4071..4895)
                     /locus_tag="VFH60_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313003.1"
                     /translation="MDNYNPGPTAGPPGNNDPNVTRYFPAGEGSPPAPQQEPVGGRVE
                     APRQAYAPPAAPGPYAAPGTQPPPQPTYQGQQYAAPQAPQQPIPGQQYQGQVWPPQHQ
                     HPHQHPHPQGVQPAAGYVAPADKHDRDRTTLGIILIGGGLLFLLNQLSAFGGFGKMVP
                     LLIGGAFLYAYFRTRQAYRIGFLIPGAILTGIGVGQVIESYAFFNLGGDVTAVTLGLG
                     FCAIWFFERRHWWSLIPGSILVLSGLSQMYLIGSLWPLVLIVIGVYLLYGQSRRKV"
     gene            complement(5024..5731)
                     /locus_tag="VFH60_04160"
     CDS             complement(5024..5731)
                     /locus_tag="VFH60_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313004.1"
                     /translation="MRRLMWKRSDVVHRPPEELFELSISRLLDPKNVPSVVSMEVSEG
                     PVGTGTTIQWSTSYKDTLASDTAVITEFEPPRRVSMHYTRRVLPAPGSTSTRRYGTIE
                     TATTTIYEPVAGGTRRTASAEVWSPDVSAVWHSLYMLAHGNFYRKQFHKSTDALLDLA
                     EGPSFRRKAISFVRQVWSGWVPFWLAFVGLLWVHASHEQLGLSGSMLQVVRVTIGLMV
                     VLALIGAYFMASFRDSA"
     gene            complement(5791..6405)
                     /gene="nadD"
                     /locus_tag="VFH60_04165"
     CDS             complement(5791..6405)
                     /gene="nadD"
                     /locus_tag="VFH60_04165"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET6313005.1"
                     /translation="MKPEVKRLGVLGGSFDPVHLAHLLLAETVREALALDLVLFVPTG
                     VQPLKQGRPVTPAEHRIKMLELATQDNPSFGVSRVDVDREGPSYTVDTVRHLREAWGG
                     EDLQMWFILGADSLASFPMWRDPSGILSQTRLAVVGRPGVSVDLATLEERLPGITASV
                     DRVEAPLLEISATDIRQRVATGRSIRYRVPDAVREYIEVNALYR"
     gene            complement(6442..7260)
                     /locus_tag="VFH60_04170"
     CDS             complement(6442..7260)
                     /locus_tag="VFH60_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET6313006.1"
                     /translation="MTPTPTLPTPETTAVPYFGFFGGGPDPLVNVLFDIARALVILLV
                     ALFIARLAKRWVVHLFARSRISLNIAVLIGNVLQALVAVLGLLPLAGVLGVDWAALVT
                     LLGAVGLGLSLAMQDLLKNVVAGIYILLEHPFKIGDRISVKDATGVVRSIELRTTILS
                     TEDGLQVVVPNNVVLNEIVTNRSVTNLHRQVIVIQVPNASLTNTSTQISTALRDFADI
                     AASPAPVVALESVTDGNASLRIEYWVPLGTQMSLTPQVVEALQARFPHANVTVA"
BASE COUNT         1383 a         2324 c         2425 g         1298 t
ORIGIN      
        1 cgttggagca gggcagcacg gcagagggtt ccgggcatag ggacgtggtc atgcgggtgc
       61 tgcgggctgc gctcgaggcc gccgatccac accgtgcggt gcgcgctgcg ctgcgggggc
      121 gcgacttctc ccatgtggcg ggtgatattt acgtgctggg cgcgggcaaa gccggggccg
      181 ccatgtccgg ggccgccgag gaagtcctgg gtgagcgcat cgccggtggg ttggtggtag
      241 ttaaggaagg gcacagcggg gacgtacggc ctgggcgcat cgaggtagtg gaggctagcc
      301 accccgtgcc cgacgagcga ggtgtgcagg ccaccgggcg gctgctggag ttggcgggcg
      361 cggcaggcga aaacgacctg gtgctctgcc tcatttcggg tggcggctcg gccctcatgt
      421 ccgcgccggc ggagggactt acgctagagg acctccgcca gaccacccag tccctgttgc
      481 gtgccggcgc taccatcaac gagttgaaca gcgcacgcaa gcacctgtcc cgcgtctccg
      541 ggggccgcct ggcacgcgcc gcctcacccg cccgcgtgct gtccctcatc ctgagcgatg
      601 tgaccggctc cccgctcgac gttatagcct ccggccccac agcgcccgac cccaccacct
      661 tccacgatgc cctcgccgtg ctcgaacgct acgccctcaa cgagaacgtc cctgccgccg
      721 tgcgtgatag gctgcgtgcg ggggccgagg gcggggtaga ggaaacgccc aagcccgacg
      781 acccgctgtt cgctcgtgtg gagaacgtca ttgtcgccag caacgtgatc gccgtggaag
      841 ccggggcagc gaaggcgcgc gagatgggac tagatgctgc tattatgtca acatacatcg
      901 agggtgaagc cgcagaggta gggctggtgc tggccggggt ggcgaaggag atagccgctt
      961 acggcaggcc ggtggacagg ccgggctgcg tgcttttcgg cggcgagacc accgtgacgg
     1021 tgcgcggcaa cggcacgggg ggacgcaata ctgagcttgc cctggcggca gcggtaggca
     1081 tcagggagat ggggcctggg gtgctggtcg cctctcttgc cacggatggc ggcgacggca
     1141 cctcccccag cgcgggagca attatagacg gcactacaat tgcaagagga gaagccctgg
     1201 gcctcgacca ccacgccgcc cttgccaaca acgacagcta cacatacctg tccagggtgg
     1261 gcgatgcgct gatgctaggc cccaccggca ccaacgtcaa cgatgtaatg gccgtattcg
     1321 tattctagtt atgagtcctc cacccacatt ctagactcaa gactcataag tcaacactca
     1381 caactctcat aactctcgcg gggggtacta tgaagctggg cggatttact ttcgtgttgc
     1441 actcgcacat gccgttctac cggggggcgg gcaagtggcc ccacggggag gaaagcctgc
     1501 acgaggtcat ggccgagtcc tacgtgccgc tggtcgaagc gctcaccgac ttgctggaaa
     1561 gcggcataca ggccaagatc acgctcggcg tcaccccggt gctcgccgag caactggccg
     1621 accctctgat cgcgagcaac ttcgaggcat acgtgcagca ggagatcgag tcggcgcaag
     1681 ccgacgtgca ccggttcgag gcgagcgggg aggcccactt cctctacctg gcccacttct
     1741 acctcgactg gtacgcccac gtgctggaga ccttccgcaa ccgcttcgac cgcgacatca
     1801 tcggggcctt gcgccgcttg caggatgccg gggctatcga gatcatcacc tcggccgcca
     1861 cccacccata cctgcctctc atgcagcgcg actccacgat cgacgcccag attgctatcg
     1921 gcaagcgggc cagcatgcgc cacttcggca gggaaccgcg cgccatctgg ctgccggagt
     1981 gcgcctaccg ccccgcctat tacgccaccg cctcggacgg cacccagtac gaacggccgg
     2041 ggctggaaag ctggctgcaa aaccacggca tcggcctctt cttcagcgag agcagcgcgg
     2101 tggagggcag tcgcgtgctg ggccgcccgg agtttaccac aaccgagctt ttcgtggaga
     2161 gaccgcgcat ggtgcaggcc gccgcctcag gctcagacat ctatggccag gtggctcagg
     2221 tggtggagga gatgggctac gaaagcgcgg gcttcgtcac cgagaggcgg gtagtgccgt
     2281 tcgagtccgg ggggcaggcg tgggagcagg gcggcacctt ccaggcgtac tacgtgcgcg
     2341 agtcggaggt agcggtggtc gggcgcaacc gcgcggtggg cgagcaggtg tgggccgcca
     2401 agctcggcta tccgggcgac gccgagtacc gcgagttcca caagaaagac gccgagcatg
     2461 gcttccagta ctggcgcgtc accgacagca agaccgacct ggcctacaag cagccctacg
     2521 acccctacaa ggccgggcag cgcatcgagg cccacgcgga ccacttcgtg tcgctgctgg
     2581 aagaacagtt ccgctggtac aacgtcaaga gcgggggcac acccggcatc atcgcctcgg
     2641 cctacgacac ggagcttttc gggcactggt ggttcgaggg ggtgcagttc ctcaaggagg
     2701 tgcttacccg cctggcgaag cacgagcgca tcgacctggt cacgacctcc gagtggctcg
     2761 aacagcgtcc gccggagcgc gtgatagacc tgccggaaag ctcctggggc cacgcgggca
     2821 cgcacgtcac ctggatcaac cccgaaacta gctggatgtg ggactcggtg cacgccgccg
     2881 agagccgcat ggaggagctg gtagagcggt tcgagagcgc tagcggcgat gtggaagcag
     2941 ccctcaacca ggccgcccgc gagcttgtgc tgctacaagc cagcgactgg gagttcctct
     3001 acacgacagg acaggcccgc cagtacgcca ccgaccgctt tatggagcac gtggaccgct
     3061 tcaacgccct ggccgacgcg gtagagcagg acggcgaacg agcggccagc ctcgcccgcg
     3121 aatacggcga gcgcgacaac ctcttccccg acatagacta ccgcctcttc gccagaaagc
     3181 aacccctcta ggcagcaacg tcgagactac accagcagta tttttcaccg cagagacgca
     3241 gagacgcaga gaacgcagag gacacgcgga gagaatgaag ggtttaggtg atgcaaaaag
     3301 gaccagggta cgtgttgttg taccctagtc cttttgcttg gaacaccatc ttaatcttgg
     3361 cgtcgttctt ggcgttcttg gcgtcttggc ggttccgtct agcttcgtct ccttagctgc
     3421 gcccgctgct acccaggccg ccggtttcgc cgccgtactg gccggggcct gcggcgtcgt
     3481 cggcgccttc ctggccgccc ttgatgccgc cggtgcgtgc gtcgaagccc tccatctcga
     3541 ccgtgaggtc ggtgccggtg cccgtttcgc caaattcacc gaactgagcg cccggccgct
     3601 ctacgccggt gccgctcgca ctatccgcgc cgccgccacc gccgctgcta ccctggccgc
     3661 caccggagcc gaaagccgag ccggagccac cttcggagtc gctggtgccg tagtcctcaa
     3721 tttcaagttc ggggttgttg cccccactgc cattacccat gctgattgtc ctcctggtaa
     3781 gagtatgagc tctatgcttg tgatgccggg tatgcgggga gcaagcttta tgccatcggg
     3841 caaccggtaa gagacggaac caccaagcct gccctgagcg tagccgaagg gacaccaagg
     3901 acgccaagag cgcgccaaga ttaagatggt gttgtcctaa gcaaaaagga ccagggtaca
     3961 atatcaagta ccctggtcct cttttcactc tctaaacctt aacgtcttgg cgcgctcttg
     4021 gcgtccttgg cgtccttggc ggttccgtct cctaatatgt tgctgcgact ctataccttg
     4081 cgacgggact ggccgtagag caggtagaca ccgatgacga tgagcaccag gggccacagg
     4141 ctgcctatga ggtacatttg cgagaggccc gacagcacca gaatgctgcc cggtatcagc
     4201 gaccaccagt gcctgcgctc gaagaaccag atagcgcaga agccgagacc caacgtaact
     4261 gccgtaacgt cgccgccgag gttgaagaag gcgtagctct cgatcacctg gccgacgccg
     4321 atgccggtga ggatagcgcc tgggatgagg aagcctatcc ggtaagcctg gcgcgtcctg
     4381 aagtaagcgt agaggaaagc cccaccgatg agcagcggga ccatcttgcc gaagccgccg
     4441 aacgccgaaa gctggttcag caggaaaagc aggccgccgc ctatcaggat gataccgagc
     4501 gtcgtgcggt cgcggtcgtg cttgtccgcg ggcgcgacgt agccggcagc aggctgaacg
     4561 ccctgcgggt gagggtgctg gtgagggtgc tggtgctggg gcggccacac ctgcccctgg
     4621 tactgctggc ctggaatggg ctgttgaggg gcctggggcg ctgcgtactg ctggccctgg
     4681 tatgttggct ggggaggcgg ttgcgtgccg ggcgcggcat acgggccggg ggccgcgggc
     4741 ggagcgtagg cctgccgtgg ggcctcgaca cgccctccaa ccggctcttg ctgcggggcg
     4801 ggtgggctac cctcaccggc cgggaaatag cgggtgacgt tggggtcgtt attgccgggc
     4861 ggccctgcgg tagggccggg gttgtagttg tccatgtctg gttctcctaa ctatcgcctg
     4921 gagtgtaagc ggatgtcttg ttttatcaaa ggcatgtacg cagccgggcg gtgcagagtt
     4981 gcaacattgc gccggaactg ctcagaagat gcatgtgatt tcgttacgcg ctatcccgga
     5041 aagaggccat gaaataggcc ccaatgaggg ccaagaccac catcagccca atagtcacac
     5101 gcaccacttg caacatggac ccggacagcc ccaactgctc gtgcgaggca tgcacccaca
     5161 acaaccccac gaacgccagc cagaaaggca cccagcccga ccagacttgc ctcacgaacg
     5221 atatggcctt gcgccggaac gacggaccct ccgccaggtc gagcaaggca tccgtgctct
     5281 tgtggaactg cttcctgtag aagttaccgt gggccagcat gtacagggag tgccagaccg
     5341 cgctcacgtc gggggaccag acctcggccg aggccgtcct gcgagtgcca cccgccacag
     5401 gctcgtagat ggtagtcgtg gcagtctcta tagtgccgta ccgccgggtc gaggtgctgc
     5461 cgggcgcggg cagaacacgg cgagtgtagt gcatagatac ccgcctgggc ggctcgaact
     5521 cggttatgac cgcggtatcg gaggctaaag tatccttgta cgaggtagac cactgtatcg
     5581 tcgtacctgt acctaccggg ccttcagata cctccatact cacaacgctg ggcacgttct
     5641 tagggtcgag tagcctgcta atcgaaagct cgaacagctc ctccggcggc ctatgcacca
     5701 catccgaccg cttccacatg agtcttctca tcagcctctc caatgcggct gccgggactt
     5761 caggcgcatg ctctactggc tgtcttttgc ctacctgtag agagcgttga cctctatata
     5821 ctcccgcacg gcgtccggca ctcgatagcg tatagagcgc ccggttgcca ccctctgcct
     5881 tatgtcggtg gccgaaatct ccagcagggg ggcctctacc cggtccaccg aggccgtgat
     5941 acccggcagc cgctcctcca acgtcgccaa gtccacgctg acgccgggcc taccgacgac
     6001 cgcgagccgg gtctgcgaca ggatgccgga ggggtcgcgc cacatcggga agctggcaag
     6061 cgagtcagcc ccgaggatga accacatttg caaatcctcc ccgccccagg cctcgcgcag
     6121 gtgccgcacg gtgtctaccg tgtaggatgg cccctcccgg tcaacgtcca cgcgtgagac
     6181 gccgaaggag ggattgtctt gggtagcaag ctcgagcatc ttgatcctgt gctcggcggg
     6241 cgtgacgggg cggccctgtt tcaaaggctg tacgccggtc ggcacgaaca gcaccaggtc
     6301 gagcgccagc gcctcgcgca ccgtctcggc caggagcagg tgggccaggt gcacggggtc
     6361 gaaggagcct cccagcacgc ccagccgttt gacttcaggc ttcatcgtct ccaaacatca
     6421 agtcctgtgt agaggtgggc gtcatgccac cgtgacgttc gcgtggggga agcgggcttg
     6481 cagggcctcc accacttgcg gggtcaggga catctgcgta ccgaggggca cccagtactc
     6541 gattcgcagg ctggcgttgc cgtcggttac gctttccagt gccaccacgg gcgcggggct
     6601 ggctgctatg tccgcgaagt cccgcagggc cgtgcttatc tgggtgctcg tgttagtcag
     6661 gctggcgttg ggcacctgta tcactatcac ctgcctgtgc aagttggtca ccgaacggtt
     6721 ggtgacaatc tcgttgagca ccacgttgtt ggggacgacc acctgtagcc cgtcctccgt
     6781 gctcaggatc gtggtgcgta gctcgatgct gcgtactacc cccgtagcgt ctttgacgct
     6841 gatgcggtcg cctatcttga aagggtgctc gagcaaaata tagatgcccg ccaccacgtt
     6901 cttgagcagg tcctgcatgg cgagggaaag acccaggccc accgcgccga gcaacgtaac
     6961 cagggcggcc caatcgacgc cgagcacccc ggccaacggc agcaagccga gcaccgccac
     7021 cagcgcctgt agaacgttgc ctatcagcac ggcgatattg aggctgatac ggctccgggc
     7081 gaacaggtgc accacccacc gcttcgccag cctggcgatg aacagcgcca ccaacagtat
     7141 gaccagggcg cgcgctatat cgaagagcac gttgaccagt gggtccgggc cgccgccgaa
     7201 gaagccgaaa tagggcacgg cggtggtctc aggtgtgggc agggtaggtg taggtgtcat
     7261 ggtgtactag tataaggcag aaccgggcga gtgacgagtg acgagtggct ggtggttagt
     7321 ggttagtggt tagtggttag tggttagtgg ttagtggtta gtggttagtg gttagtaggg
     7381 agtgccgaga cgccctgtgt gtagccctgc atgacgccct gccaatgaat
//
LOCUS       DASRWZ010000268         9199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_707269, whole genome shotgun sequence.
ACCESSION   DASRWZ010000268 DASRWZ010000000
VERSION     DASRWZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707269"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VFH60_04175"
     CDS             <1..1405
                     /locus_tag="VFH60_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017814831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6313007.1"
                     /translation="ISMVMAACGGPAATPVPATNTAGTTAEATNTAGTTVEATNTTGT
                     DAEPTDTAGTDAEPTNTAGAGGDQPYAGTTVTIFGVAADEQARLFQNEFTDFQERTGI
                     KVVYEGNKDFETLILVRVEGNNAPDIAQFAQPGLLADFVRKGNVVDLNTFMTRAELEE
                     TYNPTFLDLATVDGKMAGLWHTNDVKSLVWYPKKAFDAKGYKVPTTWDEMIALSDQIV
                     ADGSAPWCIGIESAGATGWVGTDWVEDVMLRTTTPENYDKWVRGELKFNSPEVKAAFD
                     RVGEIWFKEGYVYGGTSTILTTAFGDSVKPLFEAEPQCWLHRQASFITNFFPETAKVG
                     EDIAYFYLPPIDPAQGRPVLGSGSISAMFNDRPEVREVMKFLATGESIKAEVEAGVAV
                     APHKDADPSWYPTDTARGFAEILQNATTFRFDGSDLMPSAVGAGSFWTGIVNWVGAEG
                     ANTDQVLQEIDASWPTQ"
     gene            1562..2692
                     /locus_tag="VFH60_04180"
     CDS             1562..2692
                     /locus_tag="VFH60_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6313008.1"
                     /translation="MARTETVAELPGSPPPVGRTPTELLAWVFRLLVSLIVPLVAFFV
                     LYQGFLFLRDSDAPKLVIALVAIIWGVGGVALLFVLSNWLVERLPDAWRLRIQPFVFI
                     GPGIIMLSWFLAVPVVRTLYQSFFDRTSTNFVGFANYAYAFTAPSMQESFRNNVLWMI
                     VGTGLSVIFGLLIAVLADRSSFESIAKALIFLPMAISFVGAGIIWKFVYALSPGDEQI
                     GLLNAIVAGLGGEPQGWLVERPWNNFFLIIIMVWLQTGFAMVLLSAALKGVPGELLEA
                     ARIDGANEFQIFFRVIIPTIQGTIVTVATTILIATLKIFDIVFVMTNGNFGTEVMASQ
                     QYKQMFRFQDFGRGSAVAIILLIAVIPVMIYNLRQFRERRAF"
     gene            2692..3582
                     /locus_tag="VFH60_04185"
     CDS             2692..3582
                     /locus_tag="VFH60_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6313009.1"
                     /translation="MGEKQRRGLAKATVNTILALLCLIWSVPTLGLLVSSFRPRDEVL
                     STGWWATNPASFTTANYEQVLGGKEFVDPSLPGGVARGNDMGGSFLNSMIVTVPATLI
                     PILIAAFAAYGFAWMNFPGRKLLFTLVVALLVVPLQIALIPLLRDYTGLGLNGTFLSV
                     WLAHTGFGLPLAIYLLFNYISQLPRDILESAFIDGASHFSIFFRLILPLSTPALASFA
                     IFQFLWVWNDLLVALVFLGGNADVAVLTQRLTEMSGSRGQDWHLLTAGAFITMAVPLM
                     VFFGLQRYFVRGLLAGSVKG"
     gene            4049..4750
                     /locus_tag="VFH60_04190"
     CDS             4049..4750
                     /locus_tag="VFH60_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6313010.1"
                     /translation="MRILAVADQVEPQLYTNSVLKWLGPVDLLLSCGDLPADYLEFLY
                     STFNVPFMHVKGNHCYVPHDPVTKQCSPTSYPGILDLNGKVVEHEGLLIAGAEGSPWY
                     NGGTHQYTEQQFTLTLLRLVPGLLANKVRTGRYLDVLVTHAPPRGIHDNNDKAHRGFT
                     TLNAFIQRFRPAVLLHGHTHRYEPMLPVFTDLGATQVLNAYGHVTLQLEPAEGRAGWQ
                     LQTEQLRGVKKWMVT"
     gene            4735..5787
                     /locus_tag="VFH60_04195"
     CDS             4735..5787
                     /locus_tag="VFH60_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HET6313011.1"
                     /translation="MDGNVRHSHFVQIAAHDFERAHRKAFISQLISSLKREPNWLLSF
                     EEVKEKLPLKGQHYRGVQQVAIGDIVGSVDRYHDFNRAFLPTQTHTRPRWESVDIAAL
                     SDVILPPVQLYKVDHVYFVKDGNHRVSVAKEKGMDYIDAEVIEMPTSVSLSPTTDPRD
                     LIKLGEYARFLEQTKLDRLRPGLKIDFSTLGRYDVLIEHISAHRWYMGIDQDRPVSWE
                     EAVLDWYDNVYLPVVHTIRDNNILRGFPGHTEGDLYLWIMDHRWYLREDTGVDVGVQT
                     AALSYDEKYASWTRKVARSLRRLQEATTRPLVLSAQAITRAIRASAHAVADGHEEAVP
                     EHAVLDQESLARTEGV"
     gene            5883..7085
                     /locus_tag="VFH60_04200"
     CDS             5883..7085
                     /locus_tag="VFH60_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313012.1"
                     /translation="MKISRLTAIYTAIGLATLLAGLIVVLLLRSSPVVDNLRRAYDMA
                     RATEHHRLAPGLENRGNYVGANANAINRSQGRPPTISPTPTPTISLTPIVYPTLAPVA
                     PLSQTVDGYTVILYPVYADANSVVLTYTVQSLHPLPGKLSMCEPLPGQVTDCPPYPLA
                     PAGMSPSPIMPRAAYSPQLTGADGAILPWLRGSRLQDAQLGTPRGSLLVFDPQLSPEQ
                     LPAELKLHLTLNIAWMLVPQDDGVPVSRHVKGPFTFDFSMPVDPVRRVAEVNQTLITG
                     RGHKITVSRVIATRHHVRVFWSLGFSTQGVPTHPAGEPPDYMSYYHTCCSLSLRVGGK
                     SVGFHTGLVTPTSGYSVVDASFLDEQGNWTINTSLYSTWTGSMIYPDIPGPSFHFAMP
                     PATTSLQP"
     gene            7509..>9199
                     /locus_tag="VFH60_04205"
     CDS             7509..>9199
                     /locus_tag="VFH60_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6313013.1"
                     /translation="MDFLTRFSLRKAPIIFMVMALIVFGGWTASQQLKSELLPNIDLP
                     VLSVVTVYPGAAPDDVRRDVTEPLEKILAGSANLKSINSVSSDSVSFVTAQYQYGTNM
                     EKTQQTIQEAANRLTLPAQVQRPNVGRFNFQDIPVVAFTINTQEGGEDALANLRREVE
                     DKIVPELKAINGVNSVTVAGSGEKQVVITFDADKLRQNGLTASQITGVLQANNISFPT
                     GQVVDNGQSIPVRVTNQFNAVEDLRDLVVRPAIPAQAGAGQGAGQGAAGQGAGGGAAG
                     GQAGGQQGGAAGQGAGGQGGQTTPAQPTGPIPAVTLGEVATVELKNAAGDTISRSNGK
                     PSLSVIVYKTQNANTIQVADEVNARMDEAIKALPGSQKAILVDQSTFIKESLDGLIRE
                     GVLGAILAIVVILVFLANVRSTLITAVSIPLSVLIALILLNAFNISLNIMSLAGLAVA
                     IGRVVDDSIVVLENIYRHHFQEGESLRDAAYNGTKEVATAITSSTITTVCVFLPLGFI
                     AGLVSEFFRSFAVAVSVSLLASLIVALTVIPVLAVILLKTGRAKDPGKAAAAHAEP"
BASE COUNT         1777 a         3004 c         2636 g         1782 t
ORIGIN      
        1 aatatcaatg gtgatggcag cctgcggcgg ccccgccgcc accccggtcc ccgccaccaa
       61 cacggcgggc accactgccg aggccaccaa caccgcaggc accaccgtcg aggctactaa
      121 taccacgggg acagacgccg agcctactga cacggcgggt accgatgccg aacccaccaa
      181 taccgcaggc gcgggcggcg accagcctta cgctggcacg accgtcacca tcttcggtgt
      241 ggccgccgac gagcaggccc gcctcttcca gaacgaattt actgacttcc aggagcgcac
      301 cggcatcaag gtcgtctacg agggcaacaa ggacttcgag accctcatcc tcgtgcgcgt
      361 agaaggcaat aacgcccccg acatcgccca gttcgcacag cccggcctcc ttgccgattt
      421 cgtgcgcaag ggcaacgtgg tggacctgaa caccttcatg acccgcgccg agctggagga
      481 gacgtacaac cccaccttcc tcgacctcgc taccgtcgat ggcaagatgg caggtctgtg
      541 gcacaccaac gacgtcaaga gcctcgtctg gtaccccaag aaggcattcg atgctaaggg
      601 ctacaaagtc ccgaccacct gggatgagat gatcgctctg tccgaccaga tcgtggccga
      661 cggcagcgcc ccctggtgca taggcatcga gtcggccggc gctaccggtt gggtaggcac
      721 cgactgggta gaggacgtga tgctccgcac caccacccct gagaactacg acaagtgggt
      781 ccgcggcgag cttaagttca actcgcccga ggtcaaggct gccttcgata gggtaggcga
      841 gatctggttc aaggaaggct acgtatacgg cggcacttcc acgatcctca ccaccgcctt
      901 tggcgactcg gtgaagcccc tgttcgaagc cgagccgcag tgctggctgc accgccaggc
      961 tagcttcatc accaacttct tccccgagac ggccaaggta ggcgaggaca tcgcttactt
     1021 ctacctgccg cccatcgacc cggcgcaggg caggcccgtg ctcggctccg gctccatctc
     1081 ggccatgttc aacgacaggc ccgaggtgcg tgaggttatg aagttcctcg ctaccggcga
     1141 gtcgatcaag gccgaagtcg aggccggcgt ggctgtggct cctcacaagg atgccgatcc
     1201 tagctggtac ccgaccgata ccgctcgcgg cttcgctgag atcctccaga acgctaccac
     1261 cttccgcttc gacggctccg acctgatgcc gagcgcggtg ggtgcaggct ccttctggac
     1321 cggcatcgtg aactgggttg gcgcggaggg cgctaacacg gaccaggtct tgcaggagat
     1381 cgacgcaagc tggcccaccc agtaagcgac tcagcccgta tgggccacat acatggtgca
     1441 ggggaggggt tgcaacccct cccctgcaca tcattctcgc acttctaaga gcggccatac
     1501 ctgaaccgta acggctgctt ctatccgcaa caacctccgg gtatatagaa ggagcgtctt
     1561 tatggcacgt actgaaacgg ttgccgaact gcctggctcg ccgcccccgg tgggacggac
     1621 gccaacggag ctgctggcct gggtctttcg cctgctcgtc tcgctgattg tgcccctcgt
     1681 cgcctttttc gtcctgtacc agggctttct cttcttgcgg gatagcgacg cgcccaagct
     1741 ggttatcgcc ctcgtagcca taatctgggg cgtgggcggg gtcgccctcc tgtttgtgct
     1801 ctccaactgg ctggtcgaac gcctgcccga cgcatggagg ctgcgcatac agcccttcgt
     1861 attcatcggc cccggcataa ttatgctaag ctggttcctg gccgtgcccg tggtgcggac
     1921 cctctaccag agcttcttcg accgcacctc cactaatttc gtcggcttcg ccaactacgc
     1981 gtatgccttc accgccccca gcatgcagga gtcgtttcgc aataacgtgc tctggatgat
     2041 agtgggcacc gggcttagcg tgatatttgg cctgctcatc gccgtgcttg ccgaccgctc
     2101 gagcttcgag tcgatagcca aggcgctcat cttcttgccg atggccatct ctttcgtggg
     2161 tgccggcatc atctggaagt tcgtgtacgc cctcagcccc ggcgacgagc aaatcggcct
     2221 gctgaacgct atcgtggcgg gccttggggg tgagccacaa ggctggcttg tggagcggcc
     2281 ctggaacaac ttcttcctga tcatcatcat ggtgtggttg cagaccggct ttgctatggt
     2341 gctcctctca gcggccctca aaggcgtgcc gggcgagctg ctcgaagccg cgcgcatcga
     2401 cggcgccaac gagttccaga tcttcttcag ggtcatcata cctaccatcc agggcaccat
     2461 cgttacggtt gccacgacca tccttatcgc cacgctcaag atattcgaca tcgtgttcgt
     2521 gatgaccaac ggcaactttg ggaccgaagt catggcgagc cagcaatata aacagatgtt
     2581 caggttccag gacttcgggc gaggctccgc cgtggcgatc atcctgctca tcgcggttat
     2641 cccggtgatg atctacaacc tgcgccagtt ccgcgaaagg agggccttct aatgggtgag
     2701 aaacagcgaa ggggccttgc caaggccacc gtcaacacga tactggccct cctgtgcctg
     2761 atctggtcgg tgcccacgct cggcctgctg gtgtcgtcct tccgcccccg cgatgaagtg
     2821 ctctcgaccg gctggtgggc taccaaccca gcctccttta ccaccgccaa ctacgagcag
     2881 gtgctgggcg gcaaggaatt tgtggacccc agtctgcccg gcggcgtggc gcgcggcaac
     2941 gacatgggcg ggtcgttcct gaacagcatg atcgtcaccg tgcccgccac gttgatccct
     3001 atcctcatcg ccgccttcgc cgcgtatggc tttgcctgga tgaactttcc ggggcgcaag
     3061 ctgctattta ccctggtagt ggccttgctg gtggtgcccc tacaaattgc gttgatcccg
     3121 ctcctgaggg actacacggg gcttggcttg aacggcacct tcctgtcggt gtggctggcg
     3181 cacaccggct tcggcctgcc actcgcgatc tacctgctgt tcaactacat cagccagttg
     3241 ccacgcgaca tcctcgaatc ggccttcatc gacggcgcgt cgcacttcag catcttcttc
     3301 agactgatcc tgcccctctc tacgcccgcg ctcgcctcgt tcgccatctt ccagtttttg
     3361 tgggtctgga acgacctgct ggtggccctc gtattcctgg gcggtaacgc cgacgtggcg
     3421 gtgctcacgc aaaggctcac ggagatgagc ggctcgcgag gccaggactg gcacctgctc
     3481 acggccgggg ccttcattac gatggcggtc cccctgatgg tcttcttcgg cctccagcgc
     3541 tacttcgtac gcggcctcct cgcaggctca gtcaaaggtt gattttagat cttggatttt
     3601 ggattgatgc ttacaggagt tgagcctggt tattatgaga taccacccca agacccaggc
     3661 tctacggtca accagacgct tcctcgtcgt attcaatcca aaatccaaga ccgagcgtag
     3721 cggaggatta gcctcccgct cttgacgctc catgccgggg cacagcgttc ctattaccgc
     3781 cctcatcccc tacgttcaag actctggcct acccacctga tccttcgcta cgctcaggat
     3841 gacaaggggg agtgtggtcc tgccattcgc tgcctgattg ccggctgttg ctgcatggcg
     3901 gcgggaccac cgtcgatatt cctgtatact acaggtgcaa cgctctgcta ggcccggtac
     3961 ttgcttcaac gagtgcgcgg gctgcggagc ccattcaccc cgacgctgtt gtcgaaaacc
     4021 aagcgccgaa agctagaagc tcaaaccgat gcgtatcctc gcggtcgccg accaggtgga
     4081 gccacaactc tataccaact ctgtcctgaa gtggttaggg ccggtcgatt tgctgctgag
     4141 ttgcggcgat ctgcccgccg attacctgga gtttctttac agcaccttca acgtaccgtt
     4201 catgcacgta aaaggcaacc actgttacgt gccccacgac ccggtgacca agcagtgctc
     4261 gcccaccagc taccccggca tcctcgacct taacggcaag gtagtggagc acgaggggct
     4321 gctaatcgcg ggtgcggagg gtagcccctg gtacaacggc gggacacacc agtataccga
     4381 gcagcagttc acactcacgc tgctgcggct ggtgcccggc ctgctggcga acaaggtgcg
     4441 caccgggagg tacctggacg tgctggtgac gcacgctccc cccaggggca tacacgacaa
     4501 caatgacaaa gctcaccgag gtttcaccac cctgaacgcc ttcatacagc gcttccgccc
     4561 cgccgtcctg ctgcacggcc acacccaccg ctacgagcct atgctccccg tcttcaccga
     4621 cctcggcgct acccaggtgc tcaacgcata tgggcacgtg acgctgcaac tcgaaccggc
     4681 ggaggggcgg gccgggtggc aacttcagac cgagcaactt cgcggggtca aaaaatggat
     4741 ggtaacgtaa ggcattcgca cttcgtccag attgcggcgc acgacttcga gcgcgcccac
     4801 cgcaaggctt tcatcagcca gctaatttcc tccctcaagc gcgagcctaa ctggctgctc
     4861 tccttcgagg aagtcaaaga gaaactgccc ctcaaaggcc agcactacag gggcgtgcag
     4921 caagtcgcga tcggcgacat cgtcggcagc gtggaccgct accacgactt caaccgcgcc
     4981 ttcctgccca cacagacgca cacccgccct cgctgggaga gcgtcgatat cgccgcgttg
     5041 agcgacgtga tcctgcctcc cgtgcagctt tacaaggtgg accacgtcta cttcgtgaag
     5101 gacggcaacc accgcgtatc ggtcgccaag gaaaagggga tggactacat cgacgcggag
     5161 gtgatcgaga tgcccacttc ggtgagcctg tcgcccacca ccgacccgcg cgacctgata
     5221 aagctgggcg agtacgcgcg cttcctggag caaaccaagc tcgacaggct caggccgggc
     5281 ctgaagatcg acttcagcac cctgggccgc tatgacgtgc tgatagagca catttcggcg
     5341 caccgctggt acatgggcat cgaccaggac cgccccgtta gctgggagga ggcggtgctc
     5401 gactggtacg acaacgtcta cctgccggtg gtgcacacga tccgcgacaa caacatcttg
     5461 cgcggcttcc ccggccacac cgagggcgat ctgtacctct ggatcatgga ccaccgctgg
     5521 tacctgcgcg aggacaccgg cgtggacgtg ggggtgcaga cagccgcgct cagctacgac
     5581 gagaagtacg caagctggac gcgcaaagtg gcccgcagcc tgcgccgctt gcaggaggcc
     5641 accacgaggc cgctggtgct ctccgcccag gccattaccc gtgccatacg cgcaagcgcc
     5701 cacgccgtgg ccgacgggca cgaggaggcc gtaccggagc acgctgtgct ggaccaggag
     5761 tcgctggcgc ggacggaggg cgtgtgactt atgccaggga ccgcaccata tcgcttccgg
     5821 tcctggtaga tgctcaaggg ctatgattgg tagaccctta tgaagtaaaa tagagccagg
     5881 agatgaaaat tagcaggctc accgctattt acaccgctat tggtctcgcc accctgctgg
     5941 cggggctaat agttgtgcta ctactccggt ccagtcctgt cgttgataat ctcagacgcg
     6001 cctatgacat ggcacgagct acggagcatc acaggcttgc gcctggcctc gaaaacaggg
     6061 gcaactatgt gggagctaat gccaatgcca taaacaggtc gcaaggccgc cccccaacta
     6121 ttagccctac tcctacccca actattagcc ttactcctat cgtctacccc acgctggccc
     6181 cggtcgcgcc gttgagccag acggtcgatg gctataccgt aattttgtac cccgtgtatg
     6241 ctgatgcgaa tagcgtagtc ctgacgtata cggtgcaaag tttacacccg ttgcccggca
     6301 aactttcaat gtgcgagcct ctgccgggcc aggtcacgga ctgtcctccc taccctctcg
     6361 cccccgcagg catgtcaccc tcaccaatca tgcccagggc agcctatagc ccgcaactta
     6421 ccggggctga tggtgccatc cttccgtggc tccgtggtag ccgcttgcag gatgcgcaac
     6481 tcggcactcc aagaggctcc ctgctcgtgt ttgatcctca actgtcacct gagcagttac
     6541 ccgcggaact caagttgcac ctgacgttga acatcgcctg gatgctggtg ccgcaagacg
     6601 atggtgtccc agtatcgcgg cacgtcaagg ggccatttac cttcgatttc tccatgcctg
     6661 ttgacccggt gcggcgggta gcagaggtaa accagaccct tataacaggt cgagggcata
     6721 agattaccgt ttcgagagtg attgccacgc ggcaccatgt gcgtgtattc tggagcctgg
     6781 gattctccac gcagggagtg cctacccacc ctgccgggga gcctcccgat tacatgtctt
     6841 attaccatac ctgctgttcg ctaagcctga gagttggcgg caagtctgtt ggtttccaca
     6901 cgggcctagt aacaccgacg agcggatatt ctgtagtaga tgcctcgttc ctggacgagc
     6961 aaggtaactg gacgatcaat acatcgctct actcaacctg gacgggcagc atgatctatc
     7021 ccgatatccc cggcccctcg ttccatttcg ctatgccgcc cgctaccact tctctacagc
     7081 cataaggtcg ggacagggtc tgattgtgcg ccgtctacag ctattgttga ggcggtgaca
     7141 ggttacccag gttaccttgg ggggacgccc tgcatgacgc cctgtggcta tagccacggg
     7201 cttacaagag gcaaagtccc cttcggggac taagcaacgt catgtagtct ccgaagggga
     7261 cttcgctact gcccagcccg tggctttagc cacagggcgt catgcagggc gtcccgccgg
     7321 gcacttctcc tcatccacag tatctatcac caagagcacc aaagtcccct tgtcaccatc
     7381 aacaacattg tgtataattt cacatataca ccgaaaacgg cgtacgcttt gcaaacccgc
     7441 gactatcgtg acggcgtaat atagctgtgc ccatatcaaa cgcactttac atttggagga
     7501 cttcatagat ggacttcctt acgcggttct cgctgcgcaa ggcccctatc atctttatgg
     7561 tgatggccct tatagtgttc ggcggctgga cggcttccca gcagctcaag agcgaactcc
     7621 tgcccaatat tgacctgccg gtgctcagcg tggtcacggt ctacccaggc gcggcccccg
     7681 acgacgtgcg gcgcgacgtt accgagccgc tggagaagat actggcgggc agcgccaacc
     7741 tcaagtcgat caactccgtc tccagcgata gcgtgtcgtt cgtcaccgcc cagtaccagt
     7801 acggcacgaa catggagaag acccagcaga cgatccagga ggcggccaac cgcctgaccc
     7861 tgcccgcgca ggtgcagcgt cccaacgtgg gccgcttcaa cttccaggac atcccggtgg
     7921 tcgccttcac catcaacacc caggagggtg gcgaggacgc cctggctaac ctgcgccgcg
     7981 aagtagaaga caagatcgtg ccggagctaa aggccatcaa cggcgtgaac tcggtcacgg
     8041 tggcgggcag cggcgaaaag caggtggtca tcaccttcga cgcggacaaa ctgcggcaga
     8101 acggcctcac cgcttcgcaa atcacgggcg tgttgcaagc caacaacatc tccttcccca
     8161 cggggcaggt agtagataac ggccagtcca tcccggtgcg cgttaccaac cagttcaacg
     8221 cggtcgagga cctgcgcgac ctggttgtgc gccccgccat ccccgcgcaa gctggtgccg
     8281 ggcaaggtgc tgggcaaggt gccgccggac agggcgcagg cggtggcgcg gcagggggcc
     8341 aggcgggagg ccaacagggt ggtgctgccg gacagggtgc cggaggccag ggcggtcaga
     8401 ccacgcccgc ccaacccacg gggcctattc ccgctgtcac gctgggtgag gtcgccacgg
     8461 tggaactgaa gaacgccgcc ggggacacga ttagccgcag caacggcaag cccagcctct
     8521 cggtgatcgt ctacaagacg cagaacgcca acacgataca ggtggccgac gaggtgaacg
     8581 cgcgcatgga cgaggcgatc aaggccctcc ccggctcgca gaaggccatc ctggtggacc
     8641 agtccacctt catcaaggag tcgctcgacg gcctcatccg cgagggcgtg ctgggcgcga
     8701 tcctggctat cgtggttatc ctggtcttcc tcgcgaatgt ccgctcgacc ctcatcaccg
     8761 ccgtgtccat cccgctgtcg gtgctcatcg ccctcatctt gctgaacgcc ttcaacatct
     8821 cgctcaacat catgagcctg gcgggcctgg cggtggctat cgggcgcgtg gttgacgaca
     8881 gcatcgtggt gcttgagaac atctacagac accacttcca ggagggggag tcgctgcgtg
     8941 acgcggccta caacggcacg aaggaagtgg ctaccgccat cacttcatcg accattacca
     9001 ccgtgtgcgt gttcctgccc ctcggcttca tcgcggggct ggtgagcgag ttcttccgct
     9061 cgttcgcggt ggcggtatcc gtgtcgctgc tggcgtccct catcgtggcc ctcaccgtga
     9121 tacccgtgct ggcggtcatc ttgctcaaga cggggcgcgc caaggacccc ggcaaggcgg
     9181 ccgccgccca cgcagaacc
//
LOCUS       DASRWZ010000269         1746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_775882, whole genome shotgun sequence.
ACCESSION   DASRWZ010000269 DASRWZ010000000
VERSION     DASRWZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775882"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VFH60_04210"
     CDS             <1..1321
                     /locus_tag="VFH60_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6313014.1"
                     /translation="MVNSGPLDGMAPQDAKQAIIQLLEAEGKGRATTTFRLRDWLISR
                     QRYWGAPIPMVYCDKCGEVPVPEEMLPVVLPLDVEFRLGGESPLARVESFVNTTCPQC
                     GGPAKRETDTMDTFVDSSWYFLRFCDPRNEEGPFTPDLANYWMAVDQYTGGVEHAILH
                     LMYSRFLTKVMADAGLVNVQEPFRRLFTQGMITKDGAKMSKSKGNVVPVDFMVETKGA
                     DTARLFTLFIGPPDEDAEWSDQGADGMFRFLNRVWRLFEGSVTVVDANGAERAIADYS
                     VSDRDLLRKVHLTIRKVTSDIERFHFNTAVSAIMELSNAMQSYREAHGPETVAYSIAA
                     TNLLLLIAPMSPHIAEELWQRSGGQGSVSMQAWPQFNEELAAADVITLVIQVNGKVRD
                     KIEVSADVSEDEATTLALASPKVRQYLDGGSPKQVRYVPGRLVNIVV"
BASE COUNT          348 a          482 c          584 g          332 t
ORIGIN      
        1 aatggtaaat tcggggccgc tggatggcat ggcgccgcaa gacgcgaagc aggctatcat
       61 acagttgctg gaagccgagg ggaaggggcg ggcgaccacc accttccgcc tgcgcgattg
      121 gctgatctcg cggcagcggt actggggggc gcccatcccg atggtctact gcgacaagtg
      181 cggtgaggtg ccggtgcctg aggaaatgct accggtggtg ctgccgctgg acgtggagtt
      241 caggctgggg ggcgagtcgc cgttggcgcg ggtggagagc ttcgtcaaca ccacctgccc
      301 ccagtgtggg ggtcccgcca agcgcgagac tgacacgatg gacacgttcg tggattcgtc
      361 gtggtacttc ctgcgcttct gcgaccctcg caacgaagag ggtcctttca caccggacct
      421 ggctaactac tggatggcgg tggaccagta cacgggtggg gtggagcacg cgatcctgca
      481 cctgatgtac tcgcgcttcc tgaccaaggt gatggccgac gcgggcctgg tgaacgtgca
      541 ggagccgttc cggcggctgt tcacgcaggg catgatcacc aaggacgggg ccaagatgtc
      601 caagagcaag ggcaacgtgg tcccggtgga cttcatggtc gagaccaagg gcgcggacac
      661 ggcccgcctg ttcaccctgt tcatcggccc gcccgacgag gacgccgagt ggagcgacca
      721 gggcgcggac ggcatgttcc gcttcctgaa ccgggtgtgg cggctgttcg agggcagcgt
      781 gacggtggtg gacgcgaacg gcgcggagcg tgcgattgcc gactactcgg tctcggaccg
      841 cgacctgctg cgcaaggtgc acctgacgat acgcaaggtc acgagcgaca tagagcggtt
      901 ccacttcaac acggcggtga gcgccatcat ggagctgtcg aacgcgatgc agagctaccg
      961 ggaggcccac ggtcccgaga cggtggccta ctcgatagcg gccaccaacc tgttgttgct
     1021 gatcgcgccg atgtcgccgc atatagccga agagttgtgg cagcgttcgg gcgggcaggg
     1081 cagcgtgagc atgcaggcgt ggccgcagtt caacgaggaa ctggcggcgg cggacgtaat
     1141 cacgttggtc atccaggtta atggcaaggt gcgcgacaag atcgaggtgt ccgccgacgt
     1201 atcggaggac gaggcgacga cgctggccct ggcaagcccc aaggtgcggc agtacctgga
     1261 cggtggcagc cccaagcagg tgcgctacgt accgggtagg ctggtgaata tcgtagttta
     1321 gttggttgca ccggcattgt gttagcccgg cggtgaattg ataccggtac cggtaaaccg
     1381 ccaggtcaag cctgagaagg gagggtaggg gcttacgcta ccctcccttc ctggcgagaa
     1441 ccaaactttt agtcttaaat gttacgtaaa ttgtactatg agcactcctt gactcagata
     1501 atcgagttac ttatactagc cgagttcaat acagggtaga ggaagtaggt gcaggcgact
     1561 cgtgcatata gagtcgcctg tacgtagcat tctcccaaac ccgtcagagc aggcctttag
     1621 cctgttctgg attactgttg ctatcaaaag gagttctact gtgcgtcctc gtccccttag
     1681 gtctattttc acccgtggtg catcttcgac aaacccgcac cctaaagaac gaaatttcgg
     1741 aagtcc
//
LOCUS       DASRWZ010000270         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_570927, whole genome shotgun sequence.
ACCESSION   DASRWZ010000270 DASRWZ010000000
VERSION     DASRWZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570927"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..639)
                     /locus_tag="VFH60_04215"
     CDS             complement(61..639)
                     /locus_tag="VFH60_04215"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HET6313015.1"
                     /translation="MNNANPLVIVDYRPGWPEEFEAVARPIREALGMLALRVDHIGST
                     AVPGLAAKDIIDVQVTVRDFTPDLEAAFRQAGYSRLEFNQDHRPPTATGPDRDGEKQM
                     YRPAPGGRRINLHVRRASSGNGRYALLFRDYLRAQPLAAAAYGQVKIALAQHGPTDWD
                     LYYDVKDPVCDIIMAGAEDWAASSGWRIEHYS"
     gene            948..2321
                     /locus_tag="VFH60_04220"
     CDS             948..2321
                     /locus_tag="VFH60_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6313016.1"
                     /translation="MRAKRPVVVVALLVATVLLALGLMSGAAWAGAPTEVQKTTGREG
                     KAAAPLAPDVASFVYHEQSNALVGEGCSKYLSDPDRSADAGWQTYSAAYGLRYKSEFQ
                     ANANQWRVYYTTDGSNPGGALGAPSGTTQVVTGASVCTFSDETQSGQTVEVISASIPA
                     RSPGTTVKYILSAWHSGGGAEVFANSGACPGCVSCTQASCATLFQYTVIAGGPTATPT
                     SPAGPNPTATPPGGPCDINGRFTDVPAGSPFYEFVECLYCRGAINGYEDNTFRPNVLT
                     TRGQIAKIVALAFAFGESDPANHKFADVPRGSTFYRYIEAIASREIISGYPCSPGSAL
                     EPCDDQGRPYFRPGNNVTRGQLSKIVVIAGQQALGWTLISKSEEDRSFADVYPGSTYY
                     EYIETAFCHNMIGGYPCGGPGETCDSQNRAYFREGTNATRGQISKIVCTAVRNDAACV
                     VSRPEKK"
BASE COUNT          424 a          767 c          766 g          419 t
ORIGIN      
        1 actggtgcga cttgcactct gttcggggtc gtggcctcgt gtgcgtcata gggcgcgagg
       61 ttacgagtag tgttcgatgc gccaccccga cgaggccgcc cagtcctccg cccccgccat
      121 gatgatgtcg cacaccgggt ctttcacatc gtagtacagg tcccagtcgg tgggaccatg
      181 ttgcgccagc gctatcttga cctgcccata ggccgcggct gccaggggtt gcgcgcgtag
      241 atagtcgcgg aaaagcagcg cgtaacggcc attgccgctg cttgcccggc gcacatgcag
      301 attgatgcgg cgtccacctg gcgcggggcg gtacatttgc ttctccccgt cgcggtcggg
      361 gcctgtggct gtgggcgggc ggtggtcctg gttgaattcc aggcgcgagt agcccgcctg
      421 ccggaaagcg gcttccaagt cgggtgtaaa gtcgcggacg gtcacctgca cgtcaatgat
      481 gtcctttgcc gcgaggcccg gcaccgctgt cgagccgatg tggtccactc tgagggccaa
      541 catacccaac gcctctcgta tgggacgcgc tacggcctcg aactcctcgg gccacccagg
      601 gcggtagtct acgatgacaa ggggattggc gttattcacc tgctgctcct cggtttctgt
      661 gtgtgctctc cctgctcgat acataagtcg gttgtgcaga tcagccatgc cactataccg
      721 ttttccacca tgacgcgcac aactgcggcc tgttgcgatt ggggccaggt gctgctagac
      781 tatgcaccta gcggaggcag gcaaccggcc cggcctgtgg gtctttggtt cctgtgcatt
      841 cttcgctcgt ggaaagaaga ctatcacggt cttaggctgg gtaggtgatc agtcgtttgg
      901 cttagcgtgc gtgcggggcc gggaaggagc ggatggagat ggtggctgtg agagcaaaaa
      961 ggccggtggt cgtcgtggcc ctgctggtgg cgaccgtact gctggcgctg gggctgatgt
     1021 ccggtgcggc gtgggcgggg gcacccacag aggtgcaaaa gaccacaggc agagagggca
     1081 aagcggccgc accgctcgcg cccgatgtgg caagcttcgt gtaccacgag cagtcaaacg
     1141 ccctggtagg ggagggttgc agcaagtacc tgtccgaccc cgaccgtagc gcggacgcag
     1201 gctggcagac ctacagcgcc gcgtatgggc tgcgctacaa gtcggagttc caggccaacg
     1261 cgaaccagtg gcgcgtctac tacaccaccg atggctcgaa ccccggcggc gcgctcggcg
     1321 ccccctccgg cacgacccag gtggtaacgg gcgcgagcgt gtgcaccttc tcggacgaga
     1381 cgcagagcgg gcaaaccgtg gaagtaatct cggccagcat ccccgcgagg tcgcccggca
     1441 ctaccgtcaa gtacatcctg tcggcgtggc actcaggggg cggcgcggag gtgttcgcca
     1501 atagcggggc ctgccccggg tgcgtttcgt gcacccaggc ttcctgcgcc accctgtttc
     1561 agtacaccgt catagcgggc gggccgaccg ccacccccac atcccccgca ggacccaacc
     1621 cgacggccac accccccggc ggtccatgcg acatcaacgg ccgcttcacg gacgtccctg
     1681 ccggcagccc cttctacgag tttgtggagt gcctgtattg ccggggcgcg atcaacgggt
     1741 acgaggacaa taccttcagg cccaacgtcc tcaccacgcg agggcagata gccaagattg
     1801 tcgcgctggc gtttgccttc ggcgagtccg accccgccaa ccacaaattc gccgacgtgc
     1861 cgaggggttc caccttctac cgctacatcg aggccatcgc ctcccgcgag atcatcagcg
     1921 gctatccgtg cagccccggc agcgcgctcg agccttgcga cgaccagggc cgcccatatt
     1981 tccgccccgg caacaacgtc accaggggcc agctttccaa gatcgtggtc atcgcggggc
     2041 agcaagccct cggctggacg ctgatatcga agtcggagga agaccgctcg ttcgccgacg
     2101 tgtacccagg ctccacctat tacgagtaca tcgagacggc cttctgccac aacatgatcg
     2161 gcggctaccc ctgcggcggg ccgggcgaaa cttgcgacag ccagaaccgc gcctacttcc
     2221 gcgagggcac caacgccacg cgggggcaaa tctcaaagat agtatgtacc gccgtgcgca
     2281 acgatgccgc ctgtgtggtg tccaggcccg agaagaaata gctcaccaaa gggatagagg
     2341 ttgagaagag ggcggcgcga atagtgcagc cgccct
//
LOCUS       DASRWZ010000271         1772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_227585, whole genome shotgun sequence.
ACCESSION   DASRWZ010000271 DASRWZ010000000
VERSION     DASRWZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227585"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFH60_04225"
     CDS             <1..298
                     /locus_tag="VFH60_04225"
                     /inference="COORDINATES: protein motif:HMM:NF021482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HET6313017.1"
                     /translation="FPGLLSGGIGFYPESDLLMVAVYLQAGDRRPLFKVVSKEHPLSI
                     DQQQGIPMEKVHKWLEELEQTKTRESEEDSANKRTKNKRTKSRVSRIRRSKNDG"
     gene            291..503
                     /locus_tag="VFH60_04230"
     CDS             291..503
                     /locus_tag="VFH60_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313018.1"
                     /translation="MAETNTDLLGNPTTEQEQELLRVYESLKRLAARDDLPPCAARNV
                     RRALSSMWQATNDLHLQFEQLYEYGV"
     gene            516..1154
                     /locus_tag="VFH60_04235"
     CDS             516..1154
                     /locus_tag="VFH60_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HET6313019.1"
                     /translation="MELLLLAILTGLAYLLGSIPTGLLVARVRGVDLLAAGSGKTGAT
                     NTMNAVGKGAAAVVLAVDLVKGALAVLLAFRFPWEQNAWLAIAVAWAGAAAVLGHNYS
                     VWVRLVSGRWGGGRGIVPAMGAMLMLHPLVVLAAIVAGLAAIALTRLMVIGAIVGTIG
                     AVGVAVILVATGSLWSWFLPAVVVWALLLIAGFQDNLQRLSRGAEPKLERPR"
     gene            1169..>1772
                     /locus_tag="VFH60_04240"
     CDS             1169..>1772
                     /locus_tag="VFH60_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6313020.1"
                     /translation="MTDHTIPPAPPGDERPTIFSRRRFLTLAGGGLALAMQALAGRAW
                     AASTEERAPAAKQETLDYKIAQMLMIGFSGSTLTAGNPIVRDVRDRGVGGVFLMDYSG
                     SPISNITSPAQLKTLAAGLRDLASGWPLPLLIAVDQEGGQVARLKTRYGFPATVSQQY
                     LGSRNDMAVTRSQAQTTARTLASHGLNVNFAPVVDVNTNPR"
BASE COUNT          337 a          556 c          583 g          296 t
ORIGIN      
        1 atttcctggc ctgctgtcag gaggtatagg tttctacccg gagagcgact tgttgatggt
       61 agcggtctat ctgcaagcag gtgatagacg gccactgttc aaggttgtct ccaaagagca
      121 cccactcagc atagaccagc aacagggtat cccaatggag aaggtacaca agtggctcga
      181 agaacttgag cagacaaaaa ccagggaaag tgaggaagat agcgcaaaca agcgcactaa
      241 gaataagcgc actaagtcga gggtatcgcg catccgaagg agcaagaacg atggctgaga
      301 ccaacaccga ccttctaggg aatccaacca ccgagcagga gcaggaactc ctccgcgtgt
      361 acgagtcgct caagcggctg gcggcgcggg acgacctgcc cccctgcgct gcccgcaacg
      421 tgcggcgcgc cctctcgtcc atgtggcagg ctaccaacga cctccacttg cagttcgagc
      481 agctatacga gtatggcgtc tagcaggccg caatagtgga actgctgctg ctcgccatac
      541 tgacgggcct tgcctacctg cttggctcca tccccacggg gctgctggtg gcccgcgtca
      601 ggggcgttga cttgctggcg gcgggcagcg gcaagacggg ggcgaccaac accatgaacg
      661 ccgtaggtaa aggggcggcc gccgtggtgc tggcagtgga ccttgtaaag ggcgcgctag
      721 cggtgctgct ggccttccgc tttccgtggg agcagaacgc ctggctggcg atagcagtag
      781 cgtgggcggg ggccgccgcc gtgctggggc acaactactc ggtgtgggtg cggctcgtgt
      841 cgggcaggtg gggcggaggg cgcggcatcg tgcccgcgat gggcgctatg ctgatgctgc
      901 acccgctcgt ggtgctggcg gccatagtgg cggggttggc cgccatagcc ctcacgcggc
      961 tcatggtgat tggggcaatc gtgggcacta tcggagcggt gggcgttgcc gttatattag
     1021 tggcgacagg ctcgctatgg tcctggttcc tgcccgccgt tgtcgtgtgg gccttgctct
     1081 tgattgcggg cttccaggac aatctgcaac gcctgtcgcg aggtgcagaa ccgaagttag
     1141 agcggccacg ctagctatac tggagcgcat gacggaccac acaatcccac ccgcaccccc
     1201 aggcgacgaa cggcctacca tattctcgcg ccgccgcttc ctcacgctcg cggggggcgg
     1261 gctggccctt gccatgcagg cgctggcggg tagagcgtgg gcagcatcca ccgaggagcg
     1321 ggcaccagcc gccaagcagg aaacgctcga ctacaagata gcccagatgc tgatgatagg
     1381 tttctcaggc agtacgctga ccgccggcaa ccctatcgtg cgggacgtac gcgaccgcgg
     1441 cgtgggcggc gtcttcctga tggactacag cggctcgccc ataagcaaca tcacctcccc
     1501 cgcccagcta aagacgctcg ccgcgggcct gcgtgacctg gcgagcggct ggcccctgcc
     1561 cctgctcatc gcggtggacc aggaaggcgg gcaggtggcg cggctcaaga cccgctacgg
     1621 cttccccgcc acggtgagcc agcagtacct cggctcgcgc aacgacatgg cggtgactcg
     1681 cagccaggcc cagactaccg cccgcaccct cgcctcccat ggcctcaacg tgaacttcgc
     1741 ccccgtggtg gacgtgaaca ccaacccgcg aa
//
LOCUS       DASRWZ010000272         4869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_571027, whole genome shotgun sequence.
ACCESSION   DASRWZ010000272 DASRWZ010000000
VERSION     DASRWZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571027"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..1529
                     /locus_tag="VFH60_04245"
     CDS             498..1529
                     /locus_tag="VFH60_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6313021.1"
                     /translation="MLRVIQAGMGGWGRDWARNVLRPNEEVALVACVDADAASLALAR
                     KHLDLPAHGYFETLTAALNAVDAEAVLITASLPGHVPLAAEALNAGKHVLLEKPFAPS
                     VAEARQLVELAEERGRVLMVSQNYRFFPAVQAVRELLRSGSLGDIGPVSVDFRRYANT
                     AARQGHRHYTVPQPLLADMAIHHFDLMRAVLGQEPVEIACRAWNPAWSNFEEPAAAFA
                     TIAFDGGAVVSYRGSWVSPGRQTAWAGEWHMECHAGEVAWTSRDNLGANVDHVTVKPL
                     GKRAQRLELPAHLPIDRAGSLRAFMRAVATGEEPESSGRDNLGSIALTYAAIEASTTG
                     EPVRIERAG"
     gene            1604..2329
                     /locus_tag="VFH60_04250"
     CDS             1604..2329
                     /locus_tag="VFH60_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006054886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HET6313022.1"
                     /translation="MSTPINVTVWNEFRHERNLEHPAHAIYPEGIHEAIASGLREHPD
                     LAVRTATLDEPAQGLPPAVLESTDVLLWWGHAAHDAVPDELVAQVQQRVLDGMGLIVL
                     HSGHFSKIFKRLMGTGCELKWREGEDHERLWVIEPGHPIAEGLGECIEVGREEMYGER
                     FDIPAPETLVLVSWFTAGEVFRSGCCYTRGLGKIFYFRPGHESFPTYFHPDERRGLAN
                     AVRWAAPTRGPRTVFGKAAPRES"
     gene            2319..4238
                     /locus_tag="VFH60_04255"
     CDS             2319..4238
                     /locus_tag="VFH60_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HET6313023.1"
                     /translation="MSPNYPVQPVPFTAVQLDDPFWASRIETNRSITIPLAFEKCEET
                     GRVDNFRRTAEVLRGTPDADRTPPRLVWDDSDVYKVIEGAAYALSTLPDPAVEEYVDG
                     LVALIAASQEPDGYLYTARTVDPENPPAYAGSRRWELERKASHELYNLGHLYEAAVAH
                     HHATGKRGLLEVALKSADLLDSTFGPGKEAIWPGHQVVEIALVKLHRLTSDPRYLALA
                     KFLLDARGPDGREGSGSEYNQSHAPVTQQSEAVGHAVRATYLYAGMADVAAATGEAAY
                     TGALGRIWEDVVRKKLYITGGIGATAEGEAFGPAYDLPNLTAYCETCAAIGMVFWNHR
                     LFLTGPDARYIDVLERTLYNGLLSGVSLDGREFFYENPLESDGTHQRSPWFDCACCPS
                     NIVRFFPTLPGYVYAHDAGSIWVNLFASGTAEIDMGDGRVVTLAQETRYPWEGAVRLT
                     VSTKKPLSFALKVRIPGWARGEAVPGDLYRFVDSAEGPITLKVNGRPVDLEIERGYAN
                     LKREWRSGDVVELELPMPVRRVVAHERVEAGRGRVALQRGPLVYCVEWPDNRDSEVRS
                     LTLPDDAPLSAEQVPDLLNGVVVVRGRGLRQTQDEKGGSIPTEQDFTAIPYYAWANRG
                     PGEMLVWLRRTQSAG"
     gene            complement(4279..>4869)
                     /locus_tag="VFH60_04260"
     CDS             complement(4279..>4869)
                     /locus_tag="VFH60_04260"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HET6313024.1"
                     /translation="GLFAATGESRYRQAAEMAFAYEASWFDSARSNWADLRAYRGTRK
                     HRAAISYANYGCHGAPGIALSRVRACEVLGDDQYRQEAQLALGTTLRATASMLKIELD
                     DHSLCHGACGNLEILSAGKHLIPGEWESVERMVAELSHAHALLLAAPESVWPCGVDGE
                     TPNLMLGLAGTGHFHLRRYDASIPSALLPGHTQLPG"
BASE COUNT          905 a         1553 c         1592 g          819 t
ORIGIN      
        1 ggaagaggcg gccctggccc tggtgacgac gaaccccgcc gccgtgctgg agaacagggc
       61 gatagaggtc gcgccggagc cggtggaggt gcggaagtcg ttccttgggg ggttgttcgg
      121 gggcgggaag cgggaggggt aatgtgctgt gatagcttgg ggtgctggcc cggctgtgac
      181 gccccgcatg acgccctgcc aatgaattgg cgggcttacc agggacaaag tctcctacgg
      241 agactacatg atgttcccca gtccctgtag gggactttgc ctcttgtaag cccgccaatt
      301 cattggcagg gctaaggcca cagggcaccc ctattgcttt ccgagaatga ctgcaccctg
      361 taccgccaca agttccgtca ttgcccttag gaccccttcc catactatgc tagcttttga
      421 aatacactgt aaactagaac gtatctgcaa attatctccc cgcagataac gaacagacca
      481 tccaggagaa taacggcatg ttacgagtca tccaggcagg catgggcggc tgggggcggg
      541 actgggcaag gaacgtattg cggcccaatg aggaggtagc actcgtggca tgcgtggatg
      601 ccgacgcggc ttcgttggcc ctggctcgca agcaccttga cctgcccgcc cacggctact
      661 tcgaaaccct caccgcagct ctcaacgccg tcgatgccga ggcagtgctg ataacggcca
      721 gtctgcccgg gcacgtgcct ctggccgccg aggcgctcaa cgccggcaag cacgtgctgc
      781 ttgaaaagcc ctttgcccct tcggtagccg aggcgcggca gctggtcgaa cttgccgagg
      841 agcgcggccg cgtgctcatg gtcagccaga actaccgctt cttccctgcc gtgcaggccg
      901 tacgcgagct actgcgttcg ggcagcctgg gtgacatagg cccggtttcg gtcgatttcc
      961 ggcgttacgc caacactgcg gcgcgccagg gccaccgcca ctacacggtg ccccagccgc
     1021 tcctggccga catggcgatc caccacttcg acttgatgcg cgccgtgctg ggccaggagc
     1081 cggtcgagat cgcctgccgc gcctggaacc cggcctggag caacttcgag gagcctgcgg
     1141 cagccttcgc gaccatcgcc ttcgacggcg gcgcggtcgt gagctaccgg ggaagctggg
     1201 tcagtccggg gcggcagact gcctgggcgg gcgagtggca catggaatgc catgcgggag
     1261 aggtggcctg gactagccgt gacaacctgg gggcgaacgt agaccacgtg acggtgaagc
     1321 ccctgggcaa acgcgcccag cgtctcgaac tgcccgcaca cctgcccatc gaccgtgcag
     1381 gcagcctccg cgccttcatg cgggcggttg ctacgggaga ggagcctgaa agctccggcc
     1441 gggacaacct cggcagcatc gccctgacgt atgccgcgat tgaagcctcg accacgggag
     1501 agccggtacg catagagcgc gcgggctagc gcgccccacg actaacatgc gccctgcccc
     1561 tttcgtagca gacccggcgg gcgcgcagca gaggactacc agcatgagca cacccattaa
     1621 cgtcaccgtc tggaacgaat tccgccacga acgcaacctg gagcaccccg cgcacgcgat
     1681 ctacccagag ggcatccatg aggcgattgc ctccggcctg agggagcacc ccgacctggc
     1741 ggtgcgcacc gcgacccttg acgagcctgc gcagggactg ccgcccgccg tactggagag
     1801 caccgacgtg ttgctctggt gggggcacgc cgcgcacgat gccgtgccgg acgagctagt
     1861 ggcgcaagta cagcagcgcg tacttgacgg catgggtctg atcgtgttgc attcggggca
     1921 cttctccaag attttcaagc ggctgatggg caccggctgc gagctaaagt ggcgtgaggg
     1981 cgaggaccac gaacggctgt gggtaatcga gccgggccac cccatcgcgg aggggctagg
     2041 ggaatgtatc gaggtgggcc gcgaggagat gtacggcgag cgcttcgaca tcccggcccc
     2101 tgagaccctg gtgctggtaa gctggttcac agccggcgag gtgttccgca gcggctgctg
     2161 ttacacgcgg gggctgggga agatcttcta cttccggccc ggccacgagt ccttcccgac
     2221 ctactttcac ccggacgagc ggcgcggatt ggcgaacgcc gtgcgctggg cggcacccac
     2281 gcgaggaccg cgcacggtgt tcggaaaggc ggcaccgcgt gagtcctaac tacccggtcc
     2341 agcccgtacc cttcaccgcg gtccagttgg acgacccgtt ctgggcgtca cgcatcgaga
     2401 ccaaccggag catcactatt ccccttgcct tcgagaagtg cgaggagacg gggcgcgtgg
     2461 ataacttccg ccgcaccgcc gaggtgctca gaggcacgcc cgacgccgat agaacacctc
     2521 ccaggctggt gtgggacgac agcgacgtgt acaaggtcat agagggggct gcttatgcgc
     2581 tgagcaccct gcccgacccc gcagtggagg agtacgtgga tggcctggtg gcactcatcg
     2641 ccgcgtcaca ggagccggat gggtacctct acacggcacg cactgtcgac cccgaaaatc
     2701 cccccgcgta cgccgggagc cgtcgctggg aactggagcg caaggccagc cacgagctat
     2761 acaacctggg ccatctctac gaagccgccg tggcgcatca ccacgcgaca ggtaagcgtg
     2821 gactgctcga ggtcgcgctc aagtcggctg atttgctgga tagcaccttt ggccccggaa
     2881 aagaggccat ctggccgggg caccaggtcg tggaaatagc tctcgtcaag ctccaccgcc
     2941 tcacgagcga cccccgctac ctggctctgg cgaagttcct gctagatgcg cgcggccccg
     3001 acgggcggga gggcagcggc agcgagtaca accagtcgca cgcgcccgta acccaacaat
     3061 cggaagcggt ggggcacgcc gtgagggcca cctacctgta cgcgggtatg gcagacgtag
     3121 cggccgcgac gggtgaggcc gcttacaccg gggcgctggg gcgcatctgg gaagatgtcg
     3181 tccgcaagaa gctgtatata accgggggca tcggcgcgac cgccgagggc gaagcctttg
     3241 gccccgccta cgatttgccc aacctgacgg cctactgcga gacctgcgcg gcaatcggca
     3301 tggtcttctg gaaccaccgg ctcttcttga cgggccccga cgcccgctac atcgacgtgc
     3361 tggaacgcac cctgtataac ggcctgctat ccggtgtatc gctcgacggc agggagttct
     3421 tctatgaaaa cccgctggag tccgacggga cgcaccaacg tagcccctgg ttcgattgcg
     3481 cctgctgccc cagcaacatc gttcgcttct tccccacgct gcccggctat gtctacgctc
     3541 acgacgcagg tagcatctgg gtgaatctat ttgcgagcgg cacggcggaa atcgacatgg
     3601 gcgacgggcg ggtcgtgact ctggcccagg agacccgcta tccgtgggag ggggcagtca
     3661 ggctgaccgt tagcacgaag aagcccctct ccttcgcgct caaggtgcgc atccccggct
     3721 gggcgcgcgg tgaggcggtg ccgggcgacc tgtaccgctt tgtcgatagc gcggaggggc
     3781 ctatcacgct gaaagtcaac ggccggcccg tcgacctgga gatagaacgg ggctacgcga
     3841 acctgaaacg cgagtggcgg tccggcgacg tggtggagct tgaactaccg atgccggtcc
     3901 gccgggttgt cgcgcacgag cgggtggagg ccggtagagg cagggtggct ctgcaacgcg
     3961 gccccctcgt ctactgcgtc gagtggcccg ataaccgtga tagcgaggta cgaagcctca
     4021 cgttgcctga tgatgcgccg ttgagtgccg agcaggtgcc cgacctgctg aacggtgtgg
     4081 ttgtagtcag gggccgcggt cttcgacaaa cacaagatga aaagggaggc tctatcccga
     4141 cggagcagga ctttacggcg atcccctact acgcctgggc gaaccgcgga cctggcgaga
     4201 tgctagtctg gctacgccgc acgcagagtg ctggataata aactaagcaa cgaatatatt
     4261 atcccaccgg agcgtagtct agcctggaag ttgtgtatgc cccggtagca aagcggaagg
     4321 tatggaggca tcataccggc ggaggtggaa gtggcccgta ccggccaggc cgagcatcaa
     4381 gttgggcgtc tctccatcca cgccgcatgg ccacacgctc tcgggtgccg cgagcagtag
     4441 cgcgtgtgcg tgcgatagtt ccgcgaccat ccgctccacg gactcccact cgcccggtat
     4501 caggtgctta cccgccgata gtatctccag gttaccgcag gcgccatgac acagcgaatg
     4561 gtcgtccagt tcgattttca gcatgcttgc ggtcgcacgc aaagtcgtac ctagcgccag
     4621 ctgtgcttcc tgccggtact gatcgtcgcc taagacttcg cacgcacgca cccggctcaa
     4681 cgctattccg ggtgcgccgt gacacccgta attggcgtat gaaatcgcgg ctctatgctt
     4741 ccgggtgccg cgataggcgc gcaggtcggc ccagttagac ctcgcgctgt cgaaccagga
     4801 ggcctcgtaa gcgaacgcca tttcggccgc ttgccgatac cgcgactctc cggtggccgc
     4861 gaacaagcc
//
LOCUS       DASRWZ010000273         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_571046, whole genome shotgun sequence.
ACCESSION   DASRWZ010000273 DASRWZ010000000
VERSION     DASRWZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571046"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFH60_04265"
     CDS             complement(<1..899)
                     /locus_tag="VFH60_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313025.1"
                     /translation="MATTTLSELATYLQGQAQQTGGVTLDSTFLSSRVLGLIATDLLL
                     SSQSITLAQVTPKNVFVSGDTLYVVNAQTPASGALLHLKSVTANVAIQQTTLNNPTGY
                     DLVLQLVLPAEWNFGVSFPDLALLEAPGYLDLDTGPFFYFSSFTAGQPLPSFPAPPGT
                     LALAETVEATDSNILATGLNYYSTVKIGGIFGVIASLIHENNPLPMYGLITSTSDKTN
                     FTLKAYLTQSSVGIGFISASAPFLGVRVYYPPPDAGDDSSGGEQEARALALLDEDEAD
                     DESYPMLLYYLGCTINITSKDGET"
     gene            complement(918..2126)
                     /locus_tag="VFH60_04270"
     CDS             complement(918..2126)
                     /locus_tag="VFH60_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313026.1"
                     /translation="MTRIMAWNIERFGVNKLLNNAWDTTQSRANFIVSTIAQADPDVF
                     IIIEVQTNAVTGHGGLISDTSGAPGVWAILWHLRGVQVGAGWSVVPPLIISPAGGYSE
                     GIAVFFKSANLNFWGPYEWNGATSVAIGAGMQQNYAGTLWNGALPGTPTTNPAAGGYN
                     QDELAGKYQFFDGMGNVINFPNGNARTPWMTQFQVLATGRTLSLFSVHFPPKVGPARN
                     AFGQLAVVPEISGGLGAGEDRVVLGDFNINAGNVWEAGVFQHLTGGPAVLGGIPVSGV
                     NYAQQFNTPTSIKSVKQASTTGAPNYYGYGKQSSAGALLAIDNMFVARAGGAAGPAGG
                     AQVANRVIGTAAAPVAYTMDMISSIPAIIGGLPPGGARKKRLNTLVNYGHIGGGRGSS
                     DHMPVVIDLP"
     gene            complement(2123..>3975)
                     /locus_tag="VFH60_04275"
     CDS             complement(2123..>3975)
                     /locus_tag="VFH60_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313027.1"
                     /translation="TYVPGGLPTNWAEIILAQMGATGDQQFVMLTGVKGDLLTAFQTN
                     KMFLVVSMPSSIQDFLSHANAQIAIGNSAGNNWNFNLDPAAWSQTPGGTILIVKFYDM
                     SIKDLAGQTSLWSSPATFNQSADATGRAIQGIINAVDKSDPDFATFLNAVTDPKWNGI
                     LALNVVAPLNELPAQLQGLSAGIDKSQFFAHHIGINASKINVPAGGGDLGISNSSIFG
                     LINYTAPSGSGIEEGGGDYQFEVEQLKVLFLNSAVAGFSSIIDLQINVLFGEPCTLVG
                     SSNNVVQLYGVYQQQGPQGQGSYVFQTKSGQASVFNMTSNVLNAVQLSKGQFVTVQTV
                     GSQTEAEFLFWGLLDFKALPGFDLFTFGRENANDSPAGLSFGNLIIEMTFDNTQSPPD
                     PTFTFDAGQLAFDMAGSTARKAGFFNHFPLTVAGLTQAKQGTTPNSLGYMGVQTPLTQ
                     SSLAYPWYSLNFNLNLGAPGALAAQAGFVASLTAAWTPNLGQDYTVFTGLKLPGSSGS
                     KRQITIEGIFNITFKTLEIIALQESSTFILVLYGIGFKFLSFTFPPTGQVNFVLFGDP
                     SSTSSGGTSVGWYAAYAKPDAKKSAGSDKSGQLAGPSLMLPIDVPGGGGR"
BASE COUNT          715 a         1204 c         1250 g          806 t
ORIGIN      
        1 gtctcgccgt cctttgaggt gatattgata gtgcagccca ggtagtagag cagcatcggg
       61 tacgactcgt cgtccgcctc gtcctcatcg agcagggcca gggcgcgcgc ctcctgctcc
      121 cctcccgacg aatcgtcgcc cgcgtcggga gggggatagt agacgcggac tcccaggaac
      181 ggcgcgctcg cgctgatgaa ccctatgccg accgaagatt gagttagata cgccttgagg
      241 gtgaaattcg tcttgtccga cgtggacgta atcaggccgt acatcggcag cgggttgttc
      301 tcgtgtatga ggctggcgat taccccgaag atgccaccta tctttaccgt ggaataatag
      361 ttcaggcccg ttgccaggat attgctgtct gtggcttcga cggtttccgc cagcgccagc
      421 gtgccgggag gggcggggaa agaaggcaag ggctgacccg cggtgaacga tgaaaagtaa
      481 aagaaggggc cggtgtcgag gtcgaggtag ccgggcgctt ccagcagggc gaggtcgggg
      541 aagctgacgc cgaagttcca ctcagcaggc aacaccagtt gcaagaccag atcgtacccc
      601 gtcggattgt tgagcgtggt ctgctggatg gcgacgttgg ccgtgacgct tttcaggtgc
      661 agcagcgcac cggaggcggg tgtctgggcg tttaccacat aaagcgtgtc accgctcacg
      721 aagacgttct tgggcgtgac ctgcgccagc gtgatagact ggctcgacag caggagatcg
      781 gtcgctatca ggccgagcac ccgcgagctg aggaaggtcg agtcgagggt cacgcccccg
      841 gtctgctggg cctgcccttg cagatacgtc gctagttcgc tgagtgtggt cgtcgccatc
      901 gttgcgcttt agtcctctca gggcaggtca atgacgaccg gcatgtggtc gctgcttcct
      961 ctgccgccgc cgatgtggcc gtagttgacc agggtgttca gtcgcttctt ccgcgccccg
     1021 ccgggcggca agcctccgat gatcgcgggg atgctgctta tcatgtccat tgtgtaggcc
     1081 acaggcgcgg cagccgtacc gatcacccgg ttggcgactt gagcgcctcc cgccggaccc
     1141 gcagccccac ctgcgcgagc cacgaacatg ttgtctatag ccagcagcgc ccctgcgctc
     1201 gattgcttcc cgtagccgta gtaatttggc gcgcccgtgg tagacgcctg cttgaccgac
     1261 ttgatgctcg tcggcgtgtt gaattgctgg gcgtagttga cgccggagac gggtattcca
     1321 cccagcaccg ccgggccgcc tgtcaggtgc tggaatactc ctgcttccca cacattaccc
     1381 gcgttgatgt tgaaatcacc aagcacgacc ctgtcttcgc ctgctcccag tccgccactg
     1441 atttccggca cgacggccag ttggccgaag gcgttgcggg caggtccgac ttttggcggg
     1501 aaatggactg agaagaggct caacgtccgc ccggtggcga gcacctggaa ctgggtcatc
     1561 cacggcgtgc gagcgttgcc atttggaaaa ttgatgacgt tacccatgcc gtcgaagaac
     1621 tggtatttgc ccgccaactc gtcctggttg tagccaccgg ctgccgggtt ggtggtgggc
     1681 gtaccgggca gcgccccatt ccagagcgtg cctgcgtaat tctgttgcat gcccgcccct
     1741 atcgccacgc tggtagcgcc gttccactca tacggccccc agaagttcag gttcgcggac
     1801 ttgaagaaga ccgcgatacc ttcgctgtat ccacccgcgg ggctgataat caacggcggc
     1861 accacactcc acccggctcc cacctgcacg ccccgtaagt gccagaggat tgcccacacg
     1921 cccggcgcgc cggaagtgtc gctgatcagg ccgccatgcc ccgtcacggc gttggtctgc
     1981 acctcgatga tgatgaatac gtccgggtcg gcctgggcga tagtggagac gatgaaattc
     2041 gcccggctct gggtggtgtc ccaggcgttg ttcagcagct tgttgacccc aaaccgctcg
     2101 atgttccacg ccatgattcg tgtcatcggc caccccctcc cggtacgtct atgggcagca
     2161 tcaggctcgg ccccgcgagc tggcccgact tgtcgctgcc cgcgctcttc ttcgcgtccg
     2221 gcttggcgta agcggcgtac cacccaacgc tcgtgccgcc actggaagta gagctcgggt
     2281 ctccgaagag cacgaagttg acctgtcccg tcggcgggaa ggtgaagctc agaaacttga
     2341 agccgatgcc gtacagcacc aggatgaagg tgctgctctc ttgcagggcg attatctcca
     2401 gcgtcttgaa ggtgatgttg aatatcccct cgatggtgat ctggcgcttc gagccgcttg
     2461 agccgggcag ctttagccct gtaaagaccg tgtagtcctg gcccaggttg ggtgtccagg
     2521 ccgccgtgag gctggcgacg aacccggcct gtgcggccag agcgccgggt gcgcccaggt
     2581 tcaggttgaa gttcagcgag taccaggggt aggcgagcga ggattgcgtg agcggagtct
     2641 gcacgcccat atagcccagg ctgttcggcg tggtgccctg cttcgcctgc gtcagcccgg
     2701 ccaccgtcag cgggaagtga ttgaaaaagc ccgctttgcg cgccgtgctg cccgccatgt
     2761 cgaaggccaa ctgccccgcg tcgaaggtga acgtcggatc aggcggactc tgcgtattgt
     2821 cgaacgtcat ctcaattatc aggttgccga agctcagtcc ggctggcgag tcgttggcat
     2881 tctcgcggcc aaaagtgaag aggtcgaagc cgggaagcgc cttgaagtcg agcagccccc
     2941 agaatagaaa ctccgcctcg gtctgactcc caacggtctg cacggtgacg aactgcccct
     3001 tggatagctg caccgcgttc agcacgttgc tggtcatgtt gaagaccgat gcctgccccg
     3061 acttcgtctg gaatacgtaa gaaccctgcc cctgcggccc ctgctgctgg tagacgccgt
     3121 aaagctgcac tacgttgttc gatgacccca cgagcgtaca cggctcgccg aacagcacgt
     3181 ttatttgcag gtcaataatg cttgagaagc ctgctaccgc cgagttcagg aatagcacct
     3241 tgagctgctc cacctcgaac tggtaatccc cgcctccctc ctcaatgccc gaccctgacg
     3301 gagccgtgta gttgattaag ccgaagatag aagagttgga gatgcccagg tcccccccgc
     3361 ccgcaggcac gttgatcttc gaggcattga tgccgatgtg gtgggcaaaa aactggctct
     3421 tgtcaatgcc cgctgacagg ccctgtagct gggcaggcag ttcgttcagt ggcgctacca
     3481 cattcagggc cagtatgccg ttccacttgg ggtccgtgac ggcgttgagg aacgtggcga
     3541 agtcggggtc gctcttatca accgcgttga tgatgccctg gatcgccctt ccggtggcgt
     3601 cggcggactg gttgaaagtc gcggggctgg accagaggct agtctgcccg gcgaggtcct
     3661 tgatgctcat gtcgtagaac ttgacgatga ggatcgtgcc gcccggagtc tgggaccacg
     3721 cggcggggtc caggttgaag ttccagttat tgcccgcgct gttgccgata gcgatctgcg
     3781 cgttggcgtg cgacagaaaa tcctggatgg aggagggcat cgagacgacg aggaacattt
     3841 tattggtctg gaacgccgtc agcaggtctc ccttcacccc cgtcagcatg acgaactgct
     3901 ggtcgcccgt cgcacccatc tgcgccagta taatctcggc ccagttggtg ggcagcccgc
     3961 caggaacgta cgtgg
//
LOCUS       DASRWZ010000274         4050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1050353, whole genome shotgun sequence.
ACCESSION   DASRWZ010000274 DASRWZ010000000
VERSION     DASRWZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050353"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1781)
                     /locus_tag="VFH60_04280"
     CDS             complement(144..1781)
                     /locus_tag="VFH60_04280"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET6313028.1"
                     /translation="MNATVPRSPALNQNTLVRTLLLAFGGVALIQWLHSSVLAGLEHA
                     QADYGTLSWLRDGSLALPFVALAVLLSRRGGRDNRLGLAALYASAMGGGALLHGLFPG
                     DAHTTHDGAAISNAGLLQHVLVVMAIAGAYAYLADLVLDGLDRMSNGTLDRLPVPGRV
                     ARIVVLAVMLVGIAPALLFTGTSRVVAQTSFSDQVIHPRGSLNLFARRLPDGSMAFVA
                     PDFGNNGVRPTIEMTEGETLNIVVKNEVNADISLHVHGVHYNQDSDGTRHSNSFVPPG
                     GTRTYQWRAAPGTAGYWHYHDHVMGDDEGTGGILAGLYGGLIVRKPGEVRPAKTFVIV
                     GHDKTLNGRIYPDTPTPTARQGELVEFLVISHGNRVHTFHLHAHRWITPNRPGDPNPA
                     NPANAGSGHEDNHILAPGDSFGFMVIAGEGVGPGKWMYHCHFQDHASAMKGFFNVQPS
                     GQATNVETSRTFPETGKTVSGRFFEYWQQNGGLAQQGFPISEPFQEKSDVDGKTYTVQ
                     YFERAVFEAHPELARPYDVLLTPLGSLRYKEKYPNGR"
     gene            complement(1865..3256)
                     /locus_tag="VFH60_04285"
     CDS             complement(1865..3256)
                     /locus_tag="VFH60_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HET6313029.1"
                     /translation="MSRKLRALNTSVLIASLMAFAVAAGPAAASQASQPAGQTWTSLV
                     GNAISTEQGRPASWQSIRFFPETITINQGDSITWRFNAGNEPHNVVFLGPEPFPEQVA
                     GPPPGAPGGEGGPPGGGPPGGGPPGGPPGGGPPGGPGGPGAGGPPPQIPGNPKFWNRQ
                     GGNSYDGSQFVNSGVVAGDIPGPKEYTLSFPKAGTYLAICTLHAGPNPETGEVQGMVQ
                     TVVVQAPGSAAPKTQAQYDTENAALMKAEETRALALEAETRAMVSAPSTNPDGTRTYH
                     VIAGYANRDPQFPLSYVRFVPEGLNLNVGDTVKWTDPTGGFHNVIFGEEPTLVDIVPG
                     PDGQPQVLLNLINFLPTGQPAHTGSGFYNSGILAPPGTPPDAGGPFPMVTEYSLTFTQ
                     PGRFEYICSIHYSQGMAGFVTVASGGGNETVGMPRTGNGDAAGLLYGLLALGLALAAS
                     GVALRMRKSSQVS"
     gene            complement(3851..>4050)
                     /locus_tag="VFH60_04290"
     CDS             complement(3851..>4050)
                     /locus_tag="VFH60_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313030.1"
                     /translation="DREGFMQTDRVWVSVDDGFLRRVELSINTFDWQDGQAVAAIDTI
                     ATFVFYDYNTTMTIEAPIQTQ"
BASE COUNT          728 a         1193 c         1365 g          764 t
ORIGIN      
        1 ctcttcttcg ctgcgctcag aatgacagag gggagcttta ggtgctgatt ttccgcagtt
       61 ttgtctttgg atacttccag tcataggagc ggaaggccag agtcagcctg ggatagacag
      121 taatgccacc cgccatattt cccctatctg ccgttggggt acttctcctt gtagcgcagg
      181 ctgccgagcg gggtgagaag cacgtcgtag gggcgggcta gctccgggtg ggcctcgaac
      241 acggcgcgct cgaagtactg cacggtgtag gtcttgccgt ctacgtccga cttctcctgg
      301 aaaggctcgg agattgggaa gccctgctgg gccaggccgc cgttctgctg ccagtactcg
      361 aagaacctgc ccgaaacggt cttgccggtc tcagggaagg tgcgggaggt ctccacgttg
      421 gtagcctggc cggagggctg cacgttgaag aagcccttca tggccgaagc gtggtcctgg
      481 aagtggcagt ggtacatcca cttgcccgga cctaccccct cgcccgcaat caccatgaag
      541 ccgaagctgt cgccgggcgc caggatgtgg ttgtcctcgt ggccactgcc cgcgttggcg
      601 gggttggccg ggttgggatc gccgggccta ttgggagtaa tccagcgatg ggcgtgcagg
      661 tggaaggtgt gcacgcgatt gccgtgcgag atcaccagga actcaaccag ttcgccctgc
      721 cgcgcggtgg gcgtgggggt atcggggtag atgcggccgt tgagcgtctt gtcgtgcccc
      781 acgatcacga aggtcttggc ggggcggact tcgcccggct tgcgtacgat gaggccgccg
      841 tagagaccag ccaggatgcc gcctgtgcct tcgtcgtcgc ccatcacgtg gtcgtggtag
      901 tgccagtacc cggcggtacc gggagcggcc cgccactggt aggtgcgcgt gcccccaggc
      961 gggacgaacg agttggagtg ccgggtgccg tcgctgtcct ggttgtaatg cacgccgtgc
     1021 acgtgcaggc tgatatcggc gttcacctcg ttcttgacga cgatgttcag cgtctccccc
     1081 tcggtcatct ctatcgtggg gcgcactccg ttgttgccga aatcgggggc cacgaaggcc
     1141 atgctgccgt cgggcagcct tcgggcgaac aggttgaggc tgcccctggg gtggatcacc
     1201 tggtcggaaa agctggtctg cgcgaccacc cggcttgtcc cggtgaacag cagcgccggg
     1261 gcaatcccta ccagcatgac ggcgagcact acgatacgag ccacgcggcc gggcacgggc
     1321 aggcggtcga gcgtgccgtt ggacatacgg tcgaggccgt caagcaccag gtccgccagg
     1381 tatgcatacg cgcccgctat cgccatgact accagcacgt gctgtagcag cccggcattg
     1441 gagatagcgg caccatcgtg ggtagtgtgg gcgtctcccg ggaacaggcc gtgcaacagc
     1501 gccccgccac ccatcgccga ggcgtaaagg gcggccaggc cgagccggtt gtcccgccct
     1561 cccctgcgcg acaggagcac ggctaacgcc acgaacggca gggcgagaga gccgtctcgc
     1621 agccagctta gagtgccgta gtccgcctgc gcgtgctcca aaccggccag cacggacgaa
     1681 tgcagccact ggatgagggc cacaccgcca aacgccagga gcagcgtgcg tacgagcgtg
     1741 ttctggttga gggccgggga ccgggggacc gtagcgttca agggactcac ctttctggat
     1801 tgcggaaaag gagatgaggc cgtgctcccg ctatagagca cagcctcttc tcagcgctgt
     1861 ctaactaact tacctggctt gatttcctca tgcggagagc aacgccactc gccgccagcg
     1921 ccagcccgag ggccagcaag ccgtacagca ggcccgccgc atcaccgtta ccggtgcgag
     1981 gcatgccgac cgtctcattg ccgccaccgg aggccaccgt gacgaagccg gccatgccct
     2041 ggctatagtg gatggagcag atgtactcga agcggcccgg ctgggtgaat gtgagcgagt
     2101 actccgtgac catcgggaag ggtccgccgg catcgggcgg cgtgccgggc ggggcgagaa
     2161 tgccggagtt gtagaagcca ctgccggtgt gagcgggctg gccggtgggc aggaagttga
     2221 tcaggttgag caacacctgc ggctggccgt caggtccggg cacgatgtcc accaaggtcg
     2281 gttcctcacc gaatatgacg ttgtggaacc cacccgtggg atcggtccac tttacagtgt
     2341 cgcccacgtt gaggttaagc ccctcgggta cgaaacgcac gtaagagagg gggaactggg
     2401 gatcgcggtt ggcgtagccg gcaatcacgt ggtaggtgcg ggtgccgtca gggttggtgc
     2461 tgggggctga gaccatagcg cgggtctcgg cctctaacgc cagggccctg gtctcctcag
     2521 ccttcatcag cgccgcgttc tcggtatcgt actgggcctg ggtcttgggc gccgcgctgc
     2581 caggggcctg cacgaccacc gtctgtacca tgccctggac ctcgcccgtt tcggggttgg
     2641 ggccggcgtg cagggtgcag atagccaggt aggtgcccgc cttggggaag gaaagcgtat
     2701 attccttggg gccgggaatg tcgccggcta ccacgccgga gttgacgaac tgcgagccgt
     2761 cgtagctatt gccgccctgc cggttccaga acttggggtt gccggggatc tgcgggggcg
     2821 ggccgccagc gccgggacca ccggggccac ctggaggacc gccacccgga gggccgcccg
     2881 gaggaccacc acccggagga ccaccacccg gagggcctcc ctcaccgccg ggagcgccag
     2941 ggggcgggcc tgcgacctgc tcggggaacg gctcagggcc gaggaacact acgttatgag
     3001 gctcatttcc cgcgttgaat ctccaggtga tagagtcgcc ctggttgatg gtgatggtct
     3061 ccgggaagaa gcgtatcgac tgccaggagg cgggtctgcc ttgctccgta gagatggcgt
     3121 tgccgaccag tgaggtccag gtctggcccg cgggctggct cgcctgagaa gccgcggcgg
     3181 gacccgcggc gacggcgaaa gccatcaatg aagcgattag caccgaagtg ttgagcgctc
     3241 ttagttttcg ggacataact tccattcctc cacgatttaa cgaattgtat ctaccgtgtg
     3301 tttttgccgt acgttgcacg tgcgttctcg attgtctgag catgacgctc tatgtgtcgg
     3361 gggagaaatc accacctcct tgcgtcctgc gctctcaatt gtgcgctcgt acgctgctca
     3421 cttaccaggc cacgggctgt tcactttgtg ttcagtgtgt gcgggtggga agcggggcgt
     3481 tagagcacta ccaaatttga gattatggca tgcctattga gggcgcgagc atatagcact
     3541 gacaggcctc tacatacaac accggcggac agagtgtagc gcgaaggggg agacatgtca
     3601 atagatttgc gatattttcc acatctaccc agctacacgg gccggtgtct tgccacctct
     3661 atctaaccac aaaaggggct gtggtaggtc gcacaaaaaa ccgcacaaga agtaagacag
     3721 gcagggcacg tgggggcgga ccggagggtt tagggggagt agagtggtcc tccccctcga
     3781 tgggactgtc aacaaaaaga ggggccgctg aacagagtgt cagcggcccc tccatgcgaa
     3841 gcgcggtgcg ttattgagtc tggatggggg cctcaatcgt catcgttgtg ttgtagtcgt
     3901 agaagacaaa cgtagcgatt gtatcgatgg ccgcgacggc ttgcccgtct tgccagtcga
     3961 aggtgttgat gctaagttct accctccgca agaacccatc gtcaactgaa acccagactc
     4021 tatccgtctg cataaagcct tcccggtcgt
//
LOCUS       DASRWZ010000275         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_776543, whole genome shotgun sequence.
ACCESSION   DASRWZ010000275 DASRWZ010000000
VERSION     DASRWZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776543"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFH60_04295"
     CDS             <1..427
                     /locus_tag="VFH60_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313031.1"
                     /translation="DFPDEPGGLNPESPIAAEYEGTVQEPASNRTPAAGTAATGAALG
                     GLGGLLVGLGVLAVPGIGPLLAAGPLAGALGGMLAGGAAGGVIGGLSAIGVPEEYARD
                     YAARIEQGHTLISVRTDEVTADPAERILTVNGAENVHCD"
     gene            774..2489
                     /locus_tag="VFH60_04300"
     CDS             774..2489
                     /locus_tag="VFH60_04300"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET6313032.1"
                     /translation="MNAARFSDFARTADAVTATSKRLEKLAILAEYLAPLGDVDLAIA
                     CRFLSGQPFPPSDERTLNVGFSMASTVLVELSGIDGHEYGQLVVRLGDMGDVAERIMP
                     QQPAEPGEPLTLQSALAAFEQLASTRGTGNKAVLLRNLLAKATPPEAKYLVKLVTGDM
                     RMGLKESLVEEALARMAGVGVGELQRVNMLVGDVGETALMSRHGRLGEATMRLFHPLK
                     FMLATPAEEPRDILAEARNRAEAQLYVEDKYDGVRAQLHKQGERAELYSRTLDPVTNR
                     FPEVTAALLALPHDAIFDGEIVALHGEIESRCLPFAALQKRMGRKTVSQELMSEVPVA
                     CMVFDLLYLDGEVLLDAPLSRRKGLLAGLDLRYPLVRATGQLVPVEVFEQEARIEAEE
                     GANEADDGAKAHPLDAMFAAARDRGNEGLMVKMPGSNYSPGKRGKAWLKVKRALATLD
                     VVVTAVEWGHGKRRDMLSDYTFAVLSPEGDLLNVGKAYSGLTDAEIIEMTEWFKAHTL
                     RDFGRVRLVEPRIVIEVAFDKIQPSPRHKSGYALRFPRIVRLRPDKRPQDASTLEEVR
                     TIAEA"
BASE COUNT          519 a          836 c          874 g          425 t
ORIGIN      
        1 cgacttcccc gacgagccgg gcggcctcaa ccccgagtcg cccatcgccg ccgaatatga
       61 gggcacggtg caggaaccgg cgagcaatcg cactcccgcc gccggtacgg ctgccacggg
      121 cgcggctctg ggcggcctcg gtggcctgct tgtgggcctg ggcgtgctgg cggtgcctgg
      181 catagggcca ctgctggcgg cggggccgtt ggctggtgcg ctggggggca tgctggccgg
      241 gggcgcggca ggcggggtca taggcggcct ctcggcgata ggcgtgccgg aagagtacgc
      301 ccgcgactat gccgcccgca tcgagcaggg ccacactctc attagcgtac gtaccgacga
      361 agttaccgcc gaccccgccg agcgcatcct caccgtgaac ggcgcggaga acgtacactg
      421 cgactgattg cctgagcgcg taacacgaaa cgtgatgagg tgtaacagcc catcacgttt
      481 ttcgtttgtg acgtaagatt aagccaggac attgaagtct gtctccggaa tgaaggtatc
      541 gaatatgagc acccgaagcc ccgcctttgt cattctgagc gtagcgaaga atctcgtcct
      601 ccagcacata gttccccctt tccttcatac cgtagagagt cctatgagaa aaaagccgca
      661 tcgaggtaga ggacgagatt cttcgctacg ctcagaatga caaaggcggg gttgggtgct
      721 catatttgac acctttgctt tcgagatagg catcaaacta atgggaggca ctattgaacg
      781 cagcacgttt cagcgacttc gcccgcaccg ccgacgccgt caccgccaca agcaagcggc
      841 tggagaagct ggctatcctg gccgaatacc tcgcgcctct gggcgatgtt gacctcgcga
      901 tagcgtgcag gttcctctcg gggcagccct tcccaccctc cgatgagcgc accctcaacg
      961 tcggcttcag catggcctcc acggtgctgg tagagctttc cgggattgac gggcacgagt
     1021 acgggcagtt ggtggtgcgg cttggggaca tgggcgacgt ggccgagcgc atcatgccgc
     1081 agcaaccagc ggagccgggc gagcctttga ccctgcagtc ggccctcgcc gccttcgagc
     1141 agttggcctc cacgcggggc acggggaaca aggcggtgct gctacgcaac ctgctggcga
     1201 aagccacccc gcccgaagcc aagtacctgg tcaagctcgt cacgggcgac atgcgcatgg
     1261 ggctgaagga gtcgctggta gaggaggcgc tggcacgcat ggcgggcgtg ggcgtgggcg
     1321 agctacagcg ggtgaacatg ctcgtgggtg acgtgggaga gacggccctc atgtcacgcc
     1381 acggcaggct gggcgaggcc accatgcgcc tgttccaccc cctcaagttc atgctcgcca
     1441 cccccgccga ggagccgcgc gacatcctgg ccgaggcccg caaccgagcc gaagcgcagc
     1501 tctacgtcga ggacaagtac gatggggtgc gggcgcaact ccacaagcag ggagagcggg
     1561 ccgagctata ttcgcgcacg ttggacccgg tgaccaaccg cttcccggag gtcaccgccg
     1621 cgctcctcgc actcccccac gacgccatct tcgacgggga gatagtggcc ctgcatggag
     1681 agatcgagag ccgttgcctg cccttcgcag cgctccaaaa gcgcatgggc cgcaagaccg
     1741 tctcgcaaga gcttatgtcc gaggtgccgg tggcctgcat ggtgttcgac ctgctctacc
     1801 tggacggcga ggtgctgctg gacgcacctc tctcccgccg caaggggcta ctagcgggcc
     1861 tcgacctgcg ctaccccctc gtgagggcca ccgggcagct tgtgcccgtc gaggtgttcg
     1921 agcaagaagc gcggattgag gccgaagaag gcgcgaacga agcggatgac ggcgcgaagg
     1981 ctcacccgct ggatgccatg ttcgctgcgg cgcgggaccg tggcaacgag ggcctcatgg
     2041 tgaagatgcc cggctcgaac tattcgccgg gcaagcgggg caaggcgtgg ctgaaggtaa
     2101 agcgggccct tgccaccctc gacgtggtcg ttacggcggt ggagtggggg cacggcaagc
     2161 ggcgcgacat gctctcggac tacaccttcg ccgtgctctc ccccgaaggc gacctgctca
     2221 acgttggcaa agcctattcg ggcctcacgg acgccgagat tatagagatg accgagtggt
     2281 tcaaggccca caccctgcgc gattttggcc gggtgcgcct ggtggagcct cgcatcgtca
     2341 tcgaggtggc cttcgacaag atacagccca gccctcggca caagagtggc tacgccctcc
     2401 gcttcccccg catagtgcgc ctccgccccg acaaacgccc ccaggacgcc agcaccctgg
     2461 aagaagtgcg cactatcgcg gaggcatgaa gaagagacga aaccgccaag acgccaagga
     2521 cgccaagagc gacgccaaga acgcgccaag ggtaagaacg gttcaccgca gaggcgcaga
     2581 ggacgctgag gttacgcaga gagaataaag gtttaggtgg tgcaaagagg accagggtac
     2641 gacaacatgt accc
//
LOCUS       DASRWZ010000276         5698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_981908, whole genome shotgun sequence.
ACCESSION   DASRWZ010000276 DASRWZ010000000
VERSION     DASRWZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981908"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2139)
                     /locus_tag="VFH60_04305"
     CDS             complement(<1..2139)
                     /locus_tag="VFH60_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6313033.1"
                     /translation="MKKGFNKALASLLTYGVLVSTLALSLTGGQAIAANVASSPAAPE
                     AYSWNNPINASQGGAFYDNTPTIAADQLDGTVSIGWTRTEKGGTDAYITQASNDTLGG
                     SFRRQNLWEGEDQQMGNVKLAADAQGKRHMAWWAVDGNQVVGLISIIDANGNASDAEE
                     VPGTRGANRKNVAIATGPDNSTHVLFGRNNENIYYYHRTAQGVWDVQGEGVPTRARPV
                     DIEVGVSSLGTVMVAYKDTAAGSHNDIWTTIRRGADNWSPIESPTSPCCNGCPYDSHT
                     YQPALDRDANGGLRMVWADERCDPVSDPPSRDIYYREWTPDTGWEGHPMVRVVINSGD
                     SYYPDISVDSGGNAHLVWGDTTSSPIDYYRMFYAVGKGTSFSGVQIPADPYFGRSYQK
                     EPSVDNSPGYVHTAFASDRGDSQKENYYQYSPIDGGPAATPTPVPQPPPCPNNSFKDV
                     CVDGDQASYDAVMELSDQGVLSGYNTSPPCPNQSWINCFLPGNLITRQQLSKVIALGA
                     TVPANLQGAPHFTDVPPNNTFYQFIEYAYNAGAISGYTCGGPGEPCDSERRAYYRPLN
                     NVTRGQISKMVAEAFEFNEPVQGQSFQDVPTNSTFYEYIERLAARGILSGYPCGGTGE
                     PCGTSGKPYFRPNANVTRRQAAKIVYGAQQDANQSATPTQTQVPATSTSVATATRTAT
                     ATSTSIIPLEGSTNRAREIAVNVYNAAVTPS"
     gene            complement(2162..2929)
                     /locus_tag="VFH60_04310"
     CDS             complement(2162..2929)
                     /locus_tag="VFH60_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313034.1"
                     /translation="MSLKRLAPVLAVALLMLTAVAVIVGATGSQQYKVPAGGIVDSPN
                     ARAGVVDKGVPTIPDAPKAEPARPGAPFADDFSADSGSKYESLASAPGSWKVKGNRLQ
                     QRGDEYEELSDAPAVLTVKDVTFADNSISAHIYGIAMPTGLVFRGSDVGYYRLTLYRE
                     QANGEAKAVLEKVEGTRVTELASAPESTFAGYANQKWQTVKVTAEGNRITASVDGTQI
                     LDVADDSFSSGWAGVWATADARTQFDNVRIENASANR"
     gene            complement(3669..>5698)
                     /locus_tag="VFH60_04315"
     CDS             complement(3669..>5698)
                     /locus_tag="VFH60_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313035.1"
                     /translation="FRWSSGPVYMQLQPLGYPTFVRLFVQGVRPPGEPDAQMSLNSGG
                     RDLGTFTVPRAPGEIEVRLPLSAIAAINPRLELTTTLFQPPGDRRTLGVVFYRLEQRS
                     GPGPAVPSLWPALALLASGLLVYQAGYLASRRAFYGLVGAAVWGLSVGLLNSVARPWL
                     VFYSLYFVVPPLLFLLVLPWFRRLAPGAEPEGDGARPAETPLTERPWPLAIVVTVLAV
                     LVMAWHLLAPWVPTGDTPTDNLTWGVAFYGVLPLPIQLLGVLLFGGSLLWAWFAPLRV
                     HEEPATVEAGRPPPGFASTLRDRWLWVLAAGSVIVFSLFPVQYSEGDSEEFDRKIPIG
                     AIWRERELLDFYLKSKLWLLLRGWLPKPSQVYALVASLSGAVYVAGAWLLGRTLGRTR
                     SEGWVVVFSLVSLGNILLFFGYVESYNLVQITSLFVIWACWQYTRGRLPFGAAAALAT
                     LAPLFHGSALWWGPMVVAALVLRARQLPRAMRWKVAGADLLQGVGVGLAIVLLMGSIM
                     LIDGYDYERLQTGLSEMGGLDGRTMLPLFEAQSPYERYTFLSLPHLGAIIQEQLLTAP
                     MALPTVLAVLALAWRGTRRLVGTVAPFAVLLVGAASVFFYSISWNPDLGPRDDWDLLS
                     LPAIPLTLVAIYLLLRLPHGKARRLALTAYASVSAVHAAAWVLVHVLGIRP"
BASE COUNT         1037 a         1734 c         1894 g         1033 t
ORIGIN      
        1 cgagggcgta accgctgcgt tgtacacgtt cacggcgatt tccctggcgc ggttggtgct
       61 tccctccagt ggtatgatcg aggtgctggt ggctgtagcc gtgcgagtgg cagtagccac
      121 cgaggtagag gtagcgggta cctgggtctg ggtaggtgtc gcgctctggt tagcgtcctg
      181 ctgcgcgccg tacacgatct tggcggcctg gcggcgggtg acgttcgcgt tcgggcggaa
      241 gtaaggcttg ccgctcgtgc cgcaaggttc cccggtgcca ccgcacgggt acccggagag
      301 gataccgcgg gctgccaggc gctcgatgta ctcgtagaag gtgctgttgg tgggcacgtc
      361 ctggaagctc tggccctgca ccggctcgtt gaactcgaag gcttcagcga ccatcttgga
      421 tatctggccg cgtgtcacgt tgttcaacgg gcggtagtag gcgcgacgct cgctgtcaca
      481 tggctcaccg ggacccccgc acgtgtagcc gctgatagca ccggcgttgt aggcgtactc
      541 gatgaactgg tagaaggtgt tgttgggcgg cacgtcggtg aagtgcggcg caccttgcag
      601 gttcgcgggc accgtggcac cgagcgcgat caccttcgat agctgctgcc tggttatcag
      661 gttgcctggc aggaagcagt tgatccagct ttggttgggg catggcggcg aggtgttata
      721 cccggatagc acgccctggt cgctaagctc cattaccgcg tcgtaagagg cctggtcgcc
      781 gtccacgcac acgtccttga aggagttgtt ggggcacgga ggcggctgcg ggaccggtgt
      841 gggcgtagcc gcggggccac catctatggg gctgtactgg tagtagtttt ccttctggct
      901 gtcgccacgg tccgaggcga acgcggtatg cacgtagccg gggctgttat cgacgctcgg
      961 ctccttctgg tacgacctgc cgaagtacgg gtcggcgggg atttgcacgc cggagaagct
     1021 tgtcccctta ccaaccgcgt agaacatgcg gtagtaatcg atggggctgc tggtggtgtc
     1081 gccccacaca aggtgagcgt tgccgccact gtccaccgag atgtcggggt agtaggagtc
     1141 gccgctgttg attaccacgc gcaccatcgg atggccttcc caacctgtat ccggcgtcca
     1201 ttcgcgatag tagatgtccc ggctgggtgg gtcggaaact ggatcgcaac gctcgtcagc
     1261 ccacaccatg cgcaggccac cgttggcatc gcggtcgagc gcgggctggt aggtatgcga
     1321 gtcgtaaggg cagccgttgc agcagggaga ggtcggggac tcgataggcg accagttatc
     1381 ggcaccccgg cgtatcgtcg tccagatgtc gttgtggctg ccggcggcgg tatccttgta
     1441 ggctaccatg acggtgccca ggctggagac gcccacttcg atgtccacgg gccgcgccct
     1501 ggtgggcacc ccttcgccct gcacgtccca cactccctgt gcggtccggt ggtagtaata
     1561 gatgttctca ttgttgcgac caaagagcac gtgggtgctg ttgtccgggc cggtagctat
     1621 ggcgacgttc ttgcggttgg cgccccttgt gccgggcacc tcttcagcgt cggaagcgtt
     1681 gccgttcgcg tcgatgattg agatgaggcc gacaacctgg ttgccatcga cggcccacca
     1741 ggccatgtgc cgcttgccct gcgcatcggc ggccagcttg acgttcccca tctgctggtc
     1801 ttcgccttcc cacaggttct ggcggcggaa ggagccaccg agtgtgtcgt tgctcgcctg
     1861 ggtgatgtag gcgtcggtgc cgcccttctc ggtgcgggtc cagcctatgg aaacggtccc
     1921 gtcgagttgg tccgccgcga tggtcggagt gttgtcgtag aacgccccgc cttgtgaggc
     1981 gttgatgggg ttgttccagg aatatgcctc gggagcggcg gggctgctcg cgacattggc
     2041 cgcgattgcc tgcccacccg tcaggctaag cgccagcgtc gagaccagca caccgtaggt
     2101 gagcagggag gcgagcgcct tgttaaagcc cttcttcatg ttaaagtgtc tcctcacttc
     2161 cttagcggtt tgcagatgca ttctcgatgc ggacgttgtc gaactgggtc ctggcgtcgg
     2221 cggtagccca cacgccagcc cagccggagc taaatgagtc gtcggccacg tccagtatct
     2281 gggtgccgtc caccgaagcg gtgatgcggt tgccctccgc ggtcaccttg acggtctgcc
     2341 acttctggtt cgcgtaaccg gcgaaggtgc tttcgggcgc gctcgcaagc tcggtcaccc
     2401 tggtgccctc caccttctca agcacggcct tagcctcgcc gtttgcctgc tcgcggtaga
     2461 gggtcaggcg gtagtagccg acgtcagagc cgcggaacac caggccagtc ggcatagcga
     2521 tgccgtaaat gtgcgcgctg atgctgttgt cggcgaacgt cacgtccttc accgtcagca
     2581 cggcgggggc gtcgctcagc tcttcatatt cgtcgccgcg ctgctgaagg cggttgccct
     2641 tgaccttcca ggagccgggg gcggaagcga gcgactcgta cttgctgccc gagtcggcgg
     2701 agaagtcatc agcgaacggg gcgccggggc gggcgggttc tgccttgggc gcgtccggga
     2761 tggtagggac acccttatcg accacacccg cgcgggcgtt gggcgaatct acgatgccac
     2821 ccgcggggac cttgtactgc tgggagcctg tcgcgccaac gatcacggcg acggcagtga
     2881 gcatgagcag ggcgaccgca agtaccggcg ctaatctctt aagggacatt aaactttgct
     2941 ctcctttcca atgtgaaacc aaactgtacg ggcacgaagc catagctatt ctgttgtagg
     3001 atggcgcagt ggtacatcaa cgtgcgtggc ctccagggcg taggcagcca tacaggcatc
     3061 gcagggagat gcttcaaatc gacgagggtc attgcgcgac acagccggga ggtggtggta
     3121 agctctgcgt aaccggacca ggaggcctct ttgcaccctt cacgccgttt aggcgcccgg
     3181 cggcggctgc acgtcatgca ccggcacgcc ggtatgaagg ccggtagcaa tgtagccgta
     3241 tgctaaagtt agtgctcggc tgaaagtgcg tcgtcgcata ccgcttattg ggttggcagt
     3301 cagagtatat gagaatctta gaagcggtgt caaatctggg tacttgtcgc cggaccatgc
     3361 gggcgccgac ttttacacca aacatgatgt agagtcgccc tacagcaccg catggaatga
     3421 ccctcgcacg ccctttgagt gtgcgccaca cgtatgacga gcgagatgca gcctggttgc
     3481 gtgattgccg tactgcaaat tacgtattga tatttgcgta acccttccac ttgcaaatac
     3541 gcagggcgca cgccccggca ggcggagccg gaccaggctc gcgacagttc atggcgttgc
     3601 gccagcctcc acatccgatt ctgcgcccat tagccccaca aggtgaggtt ttccacacga
     3661 accaggtctc aggggcggat gccgagcacg tggaccagca cccaggcggc ggcatgcacg
     3721 gccgacacgc tcgcgtacgc ggtgagggca agccgccgag ctttcccatg tggcagccgc
     3781 agcagcagat atatcgctac gagggtgagt gggatggcgg ggagcgaaag caggtcccag
     3841 tcgtcgcggg ggccgaggtc ggggttccag cttatcgagt agaagaagac gctcgccgcg
     3901 cccacgagaa gcacggcaaa cggggcgact gtgccgacca gccgccttgt gccacgccag
     3961 gcgagcgcga gcaccgccag tacggtgggc agggccatgg gtgctgtcaa caactgctcc
     4021 tggattatag ctcccaagtg agggagtgag aggaaggtgt aacgctcgta gggcgactgt
     4081 gcctcgaata gaggcagcat ggtacgccca tcgaggccac ccatctcgct gagtcccgtc
     4141 tggagccgct cgtagtcgta cccgtcgatg agcatgatgc tgcccatcag caagacgatg
     4201 gcgaggccga cgcccacgcc ttgcaacagg tcggcacctg ccaccttcca ccgcatagcg
     4261 cggggcaact ggcgggcgcg caataccagt gccgctacca ccattggacc ccaccagagg
     4321 gccgagccgt ggaagagcgg ggcgagggtg gcgagtgcgg ctgcggcacc gaagggcagg
     4381 cggcccctgg tgtactgcca gcaggcccag attacgaaga ggctggtgat ttgcacgagg
     4441 ttatagctct ccacgtagcc gaagaacagc aggatgttgc ccagggatac gaggctaaac
     4501 accaccaccc aaccttcgga gcgggtgcgc cccaacgtcc tccccagcag ccaggccccg
     4561 gccacgtaca cagcacccga taggcttgct acgagcgcgt acacctgcga cggcttcggc
     4621 agccagcccc ttagcaggag ccacagcttc gacttgaggt agaagtccag cagttcccgt
     4681 tcgcgccaga tagcgcctat tggaatcttg cggtcgaact cttcgctgtc gccttccgag
     4741 tactgcaccg ggaacaacga aaacacgatg acgctacccg ccgccagcac ccacaaccag
     4801 cggtcgcgga gggtgcttgc gaagccaggt ggtggccggc ccgcctcaac ggtcgccggt
     4861 tcctcgtgca ctcgcagtgg cgcgaaccag gcccacaaca agctgccgcc gaacagcaac
     4921 acaccaagca actgtatcgg caacggtaaa acgccgtaga acgcgacacc ccacgtaagg
     4981 ttgtcggtgg gagtatcgcc ggtgggcacc caaggtgcga gcaggtgcca ggccataacc
     5041 agtacggcca ataccgttac cacaattgcg agcggccagg gacgctcggt gaggggggtc
     5101 tctgccggcc gcgccccatc cccctccggc tctgcgcccg gggccagcct gcgaaaccag
     5161 ggcagcacca gcaagaacaa cagtggcggc accacgaagt agaggctgta gaaaacgagc
     5221 cagggccgcg ccaccgaatt gagcagccct accgacaggc cccaaaccgc ggcgcctacc
     5281 agcccgtaga acgccctccg cgacgcgaga tacccggcct ggtacacgag caggccgctg
     5341 gcgagtaggg ccagcgcggg ccagagcgaa ggcaccgcag gtccggggcc gctgcgttgc
     5401 tctaggcggt aaaagaccac gcccagcgtg cggcggtcgc ccggaggttg gaagagagta
     5461 gtcgtaagtt caaggcgcgg gttgatggcc gcaatcgcgg agaggggcag gcgtacctcg
     5521 atctcgccag gggcgcgggg cacagtaaac gtgcccaggt ccctgccgcc cgaattgagc
     5581 gacatctgtg cgtcaggctc gcctggcggc cgcacgccct gcacgaacag ccggacgaag
     5641 gtggggtagc cgaggggctg caactgcata taaacggggc cgctgctcca acggaacg
//
LOCUS       DASRWZ010000277         6778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_91359,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000277 DASRWZ010000000
VERSION     DASRWZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91359"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFH60_04320"
     CDS             complement(<1..732)
                     /locus_tag="VFH60_04320"
                     /inference="COORDINATES: protein motif:HMM:NF044385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313036.1"
                     /translation="MRVLFTTQSTSSHWHPLVPLARALEGAGHEVAFAATPGFCPTIE
                     AKGFRCFPAGAYESQEARQQRKEQMAGLSGAEETFFLLRNVFAGSTAERSLPDLLNII
                     RDWRPGIVVRENTEFAGCVAAERAGIPHAVVQITAAWTFFLEAVEAPIRRLSASVGIP
                     SEQAADVPDMLYRHLLLFPRPLSLWDPAVPVPPTTRTFRYAGFSQSGAEELPGWVAEL
                     DTHGKRPTVYATLGTFDNERTDILAS"
     gene            complement(882..2726)
                     /locus_tag="VFH60_04325"
     CDS             complement(882..2726)
                     /locus_tag="VFH60_04325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET6313037.1"
                     /translation="MKRYDFEQYAAKVRSNDLSFSPDGQWVAYTNDRTGILNVWKQPV
                     HLCANGSPQMPVQLTASLDRAVVRAIWSADGKRILALADYNGTERLQICEVPPDRGWL
                     YPVTNDPGVSHEIGLARVAFPHHGEPFSPDSTRIAYACDARTPVDFDVVVRDLHTGET
                     TSVFAEGNRNYAASWLPDGRSILVVRMSQMSDHDLFLCDLETGERRHLTPHEGSVANI
                     PGPWHPDGSGFYLLTNRDREFKGLAFHRLSDDSLHWLDTPDWDVDEVAISPDGRYLAW
                     TVNEDGYSRLRLRDLSSGDTREPSGLPRGNYVCLRFSPTEPILGLYVASATQPDNLHM
                     LNVATGETWQLTHNFLGGIPLEELVEPELVRFPTFDGRTVPALLFKPKGVRPGERAPV
                     IVSIHGGPETQELPLYKDMYQYLLGRGIGVLAPNIRGSTGYGKTYQKLIYRDWGGGEL
                     RDLEHAALYLRGLDWVDPDRLGVFGGSFGGFATLSCITRLPQYWAAAVDLFGPSNLVT
                     AAASLPPSWKHRVKAWVGDPVEDYDFLVERSPINYTDNIRAPLLVIQGANDPRVKRAE
                     SDQIVERLRQTGKTVEYMLLEDEGHGAAKTANDHRIYKACVEWFEKHL"
     gene            complement(2843..5878)
                     /locus_tag="VFH60_04330"
     CDS             complement(2843..5878)
                     /locus_tag="VFH60_04330"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6313038.1"
                     /translation="MARNTPRVVDGWLSYGPGEEESPVRPVRVGSGEWWDWLHDSRAF
                     RFEDGDASFTARKEQRANGWYWYAYKRRGGKLRCVYIGATGDLTAERLSEVGRSISAA
                     EETVVGAQSPGPTGANPLLLTRLRPPMQRRRLISRPTLTRRLEAGLGGKLTLVSAPAG
                     SGKTTLLVEWTGHTELPVAWLSLDAADNDPARFLAYLIAALQGIRPEIGETALPLLRA
                     PQVVPTDPVMTVLANSIASVSHDFILVLDDYHHVQSEVVHAAVAFLLGHAPPQMHLVI
                     ASRTDPPLPIAQLRAHGQLTELRAADLGFTHDETARFLRDVMQLPASAEEVAALEART
                     EGWIAGLQLAALSSQGREGGLGAVSTFTGSHRHLLDYLAAEVLAQQPPHVRDFLLRTS
                     ILETLTAPLCEAVLGPEHASDPQATLEQIERANLFLVALDERQEWYRYHALFGEFLRS
                     YLHRTMPGEVAGLHLRAARWLHEHGFVPEAIDHALAAREYEFAASLVEQHARTMLTHG
                     ENTTLIGWLDALPEELLLARPRLCIYHAWAATYTGQCDVAEDRVNDAERAAANRASAV
                     EPQSTRGEIAAIRARLAADNSDLDRVLALSTEAMEHLPAERLDLRGHVALNLGLTYMD
                     FGEMEQTAHYLAQAHEIGRASGNLRLAIFGLRYLGVARMVQGRLHEAARLYRQALQVA
                     AEGQDGTRPPLPAAGVAYAGLGRLLYEWNYLDEAREHALRGLELGKRGGERKILLEGY
                     TVLGKTLNALGDPQGALEAMDRMVEIAGADWGHRTRLWLAQGRVAEAARWLHEKGITR
                     DSPLHYDLELEHLTAARVMVSQGNADDALYLLDRLLTSAEAQGRARSVIEALTVRAAA
                     LHSQGRADGARQSLEQALALAAPERFVRTFADEGGPIADLLEAIYSDRQPRRRSVIHI
                     PEDYLSTLLSALGRIPGEAPGHARYPSVPQELLPEPLTPREMAVLRLMSNGMSNAQIA
                     AELVIGVGTVKTHALGIYGKLDARNRTQAVARARELGLI"
     gene            complement(6092..6577)
                     /locus_tag="VFH60_04335"
     CDS             complement(6092..6577)
                     /locus_tag="VFH60_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313039.1"
                     /translation="MRLPFVVGTFVSALIGSTLGIFLVLPFGLIASEFVIFPLALAVS
                     AVLASVCAAWAGNFLASDGTRTRLLQVVGVTEGIAAIIALAILGGASLRLVLLGPLIN
                     VALACTAALALAACVATWGFRSAERDPREGRLTLGLLALAAVSVPAMIFIAWLAGLTG
                     A"
BASE COUNT         1170 a         2135 c         2317 g         1156 t
ORIGIN      
        1 ggaggcgagg atgtcggtcc tctcgttgtc gaaagtgccg agggtagcat agacggtggg
       61 ccgcttgcca tgcgtgtcta actccgcgac ccaccccggc aactcttccg cacccgactg
      121 gctgaagcct gcgtagcgga aggtgcgggt ggtgggtggg acgggaacgg cagggtccca
      181 caggctcagc gggcggggga acagcaacag gtgccggtag agcatatcgg gcacgtcggc
      241 ggcctgctcg gaggggatac ccacggaggc agacagacgc cggattggag cctccactgc
      301 ctccaggaag aaggtccagg cagcggttat ctgaacgacg gcatgaggaa tcccggcgcg
      361 ttccgccgcg acgcagcccg cgaactcggt gttctctcgc accacgatac cggggcgcca
      421 atcccggatg atattcagca ggtcgggcag gctgcgctcg gcggtggaac cggcgaacac
      481 attccgcagg aggaagaacg tctcttccgc gccgctgaga cccgccattt gctctttgcg
      541 ctgctgtcgg gcttcctgac tctcgtacgc cccggcgggg aaacaacgga agcccttagc
      601 ttctatggtc gggcagaagc caggtgtggc agcaaacgcg acctcgtgcc ccgccccttc
      661 caacgcccgc gccagcggca caagggggtg ccaatgactt gaagtcgatt gtgtagtaaa
      721 cagcacccgc acgaggacac cccttcgctc tcgccgctaa caacaatgtg acccgcacat
      781 ggtaaagagg ccatgaccgg ctacattata gcaacagcaa ggggccgaag tctcgaaagt
      841 tcctccaaat ccgcatttcg aaattcgaag ttccaaatcg tctataggtg cttttcgaac
      901 cattcgacgc acgctttgta gatgcggtgg tcgttggcgg tcttggcggc cccgtggccc
      961 tcatcctcaa gcagcatgta ctcgaccgtc ttgcctgtct ggcgcagacg ctccacaatc
     1021 tggtccgact cggccctctt gacgcgtggg tcgttcgctc cctggatcac gaggaggggc
     1081 gcgcggatgt tgtcggtgta gttgatgggg ctgcgctcca ccaggaagtc gtagtcctct
     1141 acggggtcgc ccacccaggc ctttacccgg tgcttccagg acggcgggag gctagcggcg
     1201 gcggtcacga ggttggacgg gccgaacagg tccacggccg ccgcccagta ctgtggcagg
     1261 cgggtgatgc acgacagcgt ggcaaagccg ccgaacgacc cgccgaacac ccccagccgg
     1321 tcggggtcaa cccaatcgag gccgcgcagg taaagggcgg cgtgctccag gtctctcagc
     1381 tcgccgccgc cccagtcacg gtagatcagc ttctggtacg tcttgccgta gcccgtggaa
     1441 ccccggatgt tgggtgccag caccccgatc ccacggccga gcaggtactg gtacatgtcc
     1501 ttgtacagcg gcagttcctg ggtctcaggc ccaccgtgga tggacactat cacgggcgcg
     1561 cgctcgcccg gccgcacgcc tttcggcttg aagagcaagg cgggtaccgt gcgcccgtcg
     1621 aaggtgggga agcggacaag ctccggctct acaagctcct ctagcgggat gccgcccagg
     1681 aagttgtggg taagctgcca ggtctcgccg gttgcaacgt tcagcatgtg caggttatcg
     1741 ggctgagtgg cagaggcgac gtagaggccc aggattggct cggttgggct gaagcgcagg
     1801 caaacatagt tgcccctcgg caagcccgac ggctcccgtg tgtcgccgct gctgaggtcg
     1861 cgcaagcgga ggcgggagta gccgtcctcg ttcacggtcc acgccaggta gcgcccgtcc
     1921 ggggatatag ccacctcatc aacgtcccaa tcgggcgtat cgagccagtg caggctgtcg
     1981 tcgctgagcc ggtggaaggc gaggcccttg aattcgcggt cgcggttggt cagcaggtag
     2041 aagccgctgc cgtcggggtg ccaggggccg gggatgttcg ccacgctacc ctcgtggggc
     2101 gtgaggtgcc tgcgctcgcc cgtttcaagg tcgcacagga acaggtcgtg gtcgctcatc
     2161 tgggacatgc gcaccaccag gatggagcgg ccatccggca accacgaagc cgcgtagttg
     2221 cggttgccct cggcgaatac ggaggtggtc tccccggtgt gtaggtcgcg caccaccacg
     2281 tcgaagtcta cgggggtgcg agcgtcgcag gcataggcga tgcgggtgga gtcggggctg
     2341 aacggctcgc cgtggtgcgg gaacgccact ctagccagcc cgatctcgtg cgacacaccg
     2401 gggtcgttcg ttaccgggta gagccagccc cggtcgggtg ggacctcaca gatttgcagc
     2461 cgctcggtgc cgttgtaatc ggccagggcg aggatgcgct tcccgtccgc gctccatata
     2521 gcgcggacca ccgccctgtc gaggctggcg gtgagttgca ccggcatctg cgggctgcca
     2581 ttggcacaca ggtgcaccgg ctgcttccag acgttgagta tgcccgtacg gtcgttagta
     2641 taagcgaccc actggccgtc gggactgaaa ctcaggtcgt tggagcggac cttagccgca
     2701 tattgctcga aatcgtaacg cttcatggcg cgtaccctcc ttgcctgctt atctcacata
     2761 tcgctcgcag gatgaagcaa ctcacgcgat tttccgaggg agggcgtgat gtcaggatgc
     2821 cggtgccgcg atgccgctgc ttctaaatca gccccaactc cctggcgcgg gctacggcct
     2881 gggtgcggtt gcgggcatcg agcttgccgt agatgccgag ggcgtgggtc tttaccgtgc
     2941 ccaccccgat taccagctct gcggctatct gggcgttgga cataccgttg gacatgaggc
     3001 gcagcacggc catttcgcgg ggcgtgagcg gttcgggcag caattcctgg ggcacacttg
     3061 gatacctcgc atggccgggg gcctcgcccg gtatgcggcc aagggccgat aggagggtgg
     3121 agaggtaatc ttcggggata tgtataacgg agcggcggcg gggttgccgg tctgagtaga
     3181 tggcttccaa caggtccgcg ataggcccgc cctcgtcggc gaaggtgcgc acgaagcgtt
     3241 cgggggccgc cagggccagc gcctgttcga gcgattgccg ggcaccgtcc gctcggccct
     3301 gggagtggag cgccgccgct cgcacggtta gcgcctcgat tacgctacgc gcgcggccct
     3361 gggcttccgc cgaagtcagc agcctgtcca gcaggtagag ggcgtcgtcg gcgttgccct
     3421 gtgagaccat gaccctcgcg gcggtaaggt gctcaagctc caggtcgtag tggaggggcg
     3481 agtctctggt aatgcctttc tcgtgcagcc agcgtgcggc ctcggccacc ctgccctgcg
     3541 ccagccacag ccgggtgcgg tgcccccagt ccgcgccggc aatctcgacc atgcggtcca
     3601 tcgcctcgag cgcgccttgc gggtcgccca gggcgttgag ggtcttgccc agcacggtat
     3661 agccttcgag gaggattttg cgctcgccgc cgcgcttgcc aagctcgagg ccgcgcaggg
     3721 cgtgctctcg cgcctcatcg aggtagttcc actcgtacag cagccgcccc agccccgcgt
     3781 aagccactcc tgccgccggg agcgggggcc gcgtgccgtc ctgcccttca gccgccactt
     3841 gcagagcctg cctgtagagc ctcgcggcct cgtgcaacct gccctgcacc atgcgggcca
     3901 cacccaggta gcgcaggccg aatatggcga ggcgcaggtt gccggaggcg cggcctatct
     3961 cgtgcgcctg ggcgaggtaa tgggcggtct gctccatctc accgaagtcc atgtaggtga
     4021 ggccgaggtt cagggccacg tggccgcgca ggtcgaggcg ttcggcgggc agatgctcca
     4081 tcgcctccgt ggagagggcc agcacgcggt ccaggtcgct gttgtcggcg gccaggcggg
     4141 cgcgtatggc ggctatctcg cccctggtgc tctgcggctc cacagcggaa gcccggtttg
     4201 cggccgcgcg ttcggcatcg ttcacgcggt cttccgcgac atcgcactgc ccggtatagg
     4261 tagcggccca ggcgtggtag atgcagaggc ggggccgggc cagcagcaac tcttcaggca
     4321 gagcgtccag ccagcctatg agggtcgtgt tctcgccgtg ggtgagcatc gtccgggcgt
     4381 gctgctctac cagggaggcc gcaaactcgt actcgcgggc ggcaagggcg tggtcgatag
     4441 cttccggcac aaacccgtgc tcgtgcagcc accgtgcggc ccgcaggtgc aagcccgcca
     4501 cctctccggg catggtgcga tgcaggtagc tccgcaggaa ctcgccaaac agcgcgtgat
     4561 agcggtacca ctcctgccgc tcgtccagcg ccaccaggaa caggttggcg cgttcgattt
     4621 gctcaagagt ggcttgaggg tcgctcgcat gctcgggacc cagcactgct tcgcacaggg
     4681 gggcggtgag ggtttccagt atggaggtcc gcagcaggaa gtcccgcacg tgggggggtt
     4741 gttgtgccag cacttcggcg gccagatagt cgagcaggtg gcggtggctg ccggtgaagg
     4801 tggagacggc ccccagcccg ccctcccggc cctgcgagga gagggccgcc agttgcagcc
     4861 cggctatcca cccctcggtg cgcgcctcga gggcggccac ttcctcagcg gaggcgggta
     4921 gctgcatcac atcgcgcagg aagcgggcgg tctcgtcgtg ggtgaagccg aggtcggcgg
     4981 cgcgaagctc ggttagctgg ccgtgggcgc gtagctgggc gatggggagg ggagggtcgg
     5041 tgcgggaggc gattaccagg tgcatctggg gcggggcgtg gccgagcagg aacgccaccg
     5101 ccgcgtggac cacctccgac tgcacgtggt ggtagtcgtc gagcaccagg atgaagtcgt
     5161 gggacacgga ggctatggag ttcgccagca cggtcatcac ggggtcggtg ggcacgacct
     5221 gaggggcgcg cagcaggggc agggcggtct cgcctatttc ggggcgtatg ccctggaggg
     5281 cggcaatcag gtaggcgagg aagcgggcag ggtcgttgtc cgccgcgtcg agggagagcc
     5341 aggcgacggg tagctccgtg tggccggtcc actcgaccag gagggtggtc ttgcctgagc
     5401 ctgcgggggc cgagacgagg gtgagcttgc cgccaagccc agcctccaag cgccgtgtga
     5461 gggtgggccg cgagattagc ctgcgccgct gcatgggggg ccgcagcctg gtgagaagca
     5521 gcggattggc accagtaggg ccgggcgact gagcacccac aacggtttct tccgcggctg
     5581 agatggaccg gcccacctcg gacagccgct ccgcagtgag gtccccagtc gcgccgatgt
     5641 agacgcaacg cagcttgccc ccacgccgtt tataggcgta ccagtaccag ccgttggctc
     5701 gctgctcctt gcgggcggtg aaagaagcgt cgccgtcctc gaagcggaag gcgcggctgt
     5761 cgtgcagcca gtcccaccac tcgcccgagc cgacccgcac cggccgtacc gggctttcct
     5821 cttcgccggg gccgtagctc aaccaaccgt caacaaccct gggggtatta cgcgccatgc
     5881 agacctccca agccagttta tagagcgaaa tgaagtaagg taagaccagg cagtaattac
     5941 ttatcactac tacttgtagc acgcgccaat cgccctaacg agtaatgcgc cgccgccaga
     6001 ccccggctag actggtagca gggcaccccg ctccttctag acgcgcccac tgacagaggg
     6061 acgtggggtg ctgttttact taaggcagcg gctatgcgcc cgtcagccct gctaaccacg
     6121 cgatgaagat catagcgggc acgctgaccg ccgctagagc gagcaggccc agggtgaggc
     6181 gtccctctcg tgggtctcgc tcagcactac gaaaccccca cgttgccacg caggccgcga
     6241 gcgcgagggc cgccgtgcaa gctaaggcga cgttgatgag cggccccagc aggaccagcc
     6301 tgagcgacgc cccacccagg atggcgaggg ctatgatagc ggcaatccct tccgttacgc
     6361 ccaccacctg caagagccgc gtcctggtgc cgtcgctcgc caggaagtta cccgcccagg
     6421 cagcgcatac ggaggcgagg actgcggaga cggccagggc gagcggaaat atgacgaact
     6481 cggaggctat caggccgaag ggcaagacca ggaagatacc gagcgtgctg ccaataagcg
     6541 cggatacaaa cgtgcctact acgaagggga ggcgcatcgg gtttacctac tttagttttc
     6601 taacaggcca tttgataggc taattacgtc aggctgaagc agtgccgaca tgccacaagc
     6661 aacagcacca aagtcacctt gtcatcctga gcgcagcgaa ggatcagcca ccggcgtttg
     6721 actcttccta cttgagcacc acttttcctc tagccgccag catccccttc gttgcaaa
//
LOCUS       DASRWZ010000278         1835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_365861, whole genome shotgun sequence.
ACCESSION   DASRWZ010000278 DASRWZ010000000
VERSION     DASRWZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365861"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="VFH60_04340"
     CDS             complement(<1..1402)
                     /locus_tag="VFH60_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HET6313040.1"
                     /translation="MDNIPDLYSNPYPSTRSVTIAREGVVATSQPLAVQAGISMLQRG
                     GNAVDAALATAIALTVVEPTSNGIGSDAFALVWDGERLHGLNGSGRAPGGLALEAINA
                     AGHDSMPTRGWLPVTVPGTPAAWRDLHARFGRLDFADLFEPAIYYAERGYPLSPVLAA
                     GWGYAARAYAANTTPEFRGWHDTFTPAGHIPQAGDMWASPGHAATLRRIANSNADDFY
                     NGDLARQIASFARDTGGLITEADLAGHTSTWVDPISTTYRGYEAWEIPPNGQGITALI
                     GLNILEGFDMASMPRDTAESFHLQIEALKLAFADAHRYVADPEHADVPTARLLDKGYA
                     QGRRALIGEQALDPAPGDPNSGGTVYLCAVDGDGLMVSFIQSNYMGFGSGVVVPGTGI
                     ALQNRGHGFSMERGHPNFVQPGKRPYHTIIPGFLTREGQAVGPYGVMGGFMQPQGHMQ
                     MVVNLVDYGMNPQASLD"
BASE COUNT          317 a          587 c          587 g          344 t
ORIGIN      
        1 cgtccaggct ggcctgtggg ttcatgccgt agtccacaag gttcactacc atctgcatgt
       61 gcccctgagg ctgcatgaag cctcccatga cgccgtacgg ccccacggcc tggccctccc
      121 gcgtgaggaa gccggggatg atagtgtggt aggggcgctt gcccggctgc acgaagttag
      181 ggtgcccccg ttccatgctg aagccgtggc cccggttctg tagcgcgatc cccgtgcccg
      241 gcaccaccac gcccgacccg aagcccatgt agttcgactg tatgaagctc accatcagcc
      301 cgtcaccgtc caccgcgcac aggtacacgg tgccgccgga gttggggtcg ccgggggcgg
      361 ggtccagcgc ctgctcgcca atgagggcgc gccgcccctg tgcgtaaccc ttgtccagca
      421 agcgagcggt cggcacgtcg gcgtgttcgg ggtcggcaac atagcggtgg gcgtccgcaa
      481 acgccagctt gagcgcctct atttgcaggt ggaacgactc ggcggtatcg cgtggcatgg
      541 aggccatgtc gaagccttcc aggatgttga gcccgatcag cgcggtgata ccctggccgt
      601 tgggcggtat ctcccacgcc tcgtagcccc tgtaggtcgt ggagatgggg tccacccagg
      661 tgctcgtgtg ccccgccagg tccgcctcgg tgataagccc gcccgtgtcc cttgcgaagc
      721 tcgctatctg ccgcgccagg tccccattgt agaagtcgtc ggcgttagag ttggcgatgc
      781 gccgcaaagt agccgcgtgt ccggggctgg cccacatgtc cccggcttga gggatatgtc
      841 ctgcgggcgt gaaagtgtcg tgccaacccc tgaactccgg cgtcgtgttg gcggcataag
      901 ccctggccgc gtagccccaa cccgccgcca gcacgggcga cagtggatag ccgcgctcgg
      961 cgtagtaaat ggcaggctcg aacaggtccg cgaagtcgag gcggccaaag cgggcgtgca
     1021 ggtcgcgcca ggcggcgggg gtgcccggca cagtcacggg cagccagcct ctcgtgggca
     1081 tggagtcgtg cccggcggcg tttatcgcct ccaacgcaag ccccccaggc gcgcggcccg
     1141 acccgttcag cccgtgcagc ctctccccgt cccacaccag cgcgaaagca tccgagccga
     1201 tgccattgga cgtaggctcc accacggtga gggcgatagc cgtcgccagg gcagcgtcca
     1261 cagcgttgcc gccccgctgc aacatggaga taccggcctg caccgccagc ggctggctgg
     1321 tagccaccac gccctcgcgg gcaatcgtca cgctgcgggt cgagggatag gggttgctgt
     1381 aaaggtcggg gatgttgtcc acggcggcac ctcctggggt ttgtgggtga aattataacc
     1441 gaggcgcgaa aatcagtcag caggtgagaa tgagacgaaa ccgccaagac gccaaggacg
     1501 ccaagaacga cgccaagata gaggatggtg ttcaaagcaa aaggaccagg atacatgctg
     1561 ttgtaccccg gtccttattg taccgtctaa acctttattc tctttgcacg tcctttgcgt
     1621 tctttgcgtt ctttgcgttc tttgcgtttt cgtctcttgg cgcgctcttg gcgtttttgg
     1681 cgtcttggcg gtttcgtcta atcaccgttt gccgccacgc gcccgctgtg atatgattcg
     1741 tgggttagct cagaaggaga cacccacaac atgcagacag aagagactac tacgcgacag
     1801 acccctgtcg tctatatcgc ggacctgaag gacca
//
LOCUS       DASRWZ010000279         3216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_982164, whole genome shotgun sequence.
ACCESSION   DASRWZ010000279 DASRWZ010000000
VERSION     DASRWZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982164"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="VFH60_04345"
     CDS             <1..1382
                     /locus_tag="VFH60_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6313041.1"
                     /translation="DAAIRAINTARVDYYLMKPWDPPDERLYPIVDDLLDDWLAAYRP
                     AFEGIRIVSHRWSARAHNLKDFLARNQIPYQVLDIEADEQAQKLVELAGTGPDALPLV
                     LFPDGTHMVDPEPLAVAAKVGMRTRAETEFYDLIIVGGGPAGLAAAVYGGSEGLRTLM
                     IEREAPGGQAGTSSRIENDLGVPAGLSGAELARRALTQATRFGVEVVSLQEVCGLKVK
                     DPYRTVQFTDGSEVTCYSLVVATGVQYRKLEAPGIDRLLGAGVFYGAAMTEAMACQDQ
                     HVYIVGGANSAAQAAMYFVNYAEDVTLLVRGNSLSAGMSQYLVDRIESAPNIHVRLNS
                     SVAAVDGDGRLETITIADSATGQTDTVPAGAMFIFIGASPRTEWLDGVVERDRSGFVL
                     SGLDLVREGQRPRGWTRDRDPFLLETNVPGVFVAGDVRHDSVKRIASAVGDGAMAVQF
                     VHRYLAEVR"
     gene            1472..1756
                     /locus_tag="VFH60_04350"
     CDS             1472..1756
                     /locus_tag="VFH60_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET6313042.1"
                     /translation="MQEEIAECSHLGEVQRVRPSARGCEDCLKTGDEWVHLRECMSCG
                     YIGCCNDSKNTHATKHFHASGHPIIKSFEPGEDWGWCYVDEVYFERLPTR"
     gene            1872..>3216
                     /locus_tag="VFH60_04355"
     CDS             1872..>3216
                     /locus_tag="VFH60_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET6313043.1"
                     /translation="MQGTLPVGDAPDRKPGWPRRRYVVVGLGLMLLVAASAIAILVWL
                     RPAGPAPLKPLPDVAELASNYRLSQTLTDAFGPQSWSPDGRYLVTYSASTYGLQVHDL
                     GGPSPRTWSVPAEASYSYRSIGIAEKAIWSPDSRLLAVDEARSSVPYTNTVVEPNSAL
                     PYTITVVDAEKGTVRNTIRVLGPPELAATPTPAGRSRTAVVQTFHQLVGWADNGRSLV
                     TLLFVERREDVARNPGYSLFDPTPQPDATPVPPSFSVYFYVHDAATGDMVSSMQVYFT
                     TAYPSLYGLALSPDGNTLAFATTDRTPRSEGSTSQVNLSLWDLRAGRLKHRVEGLGDE
                     VLAPFPGIWDQFLAWSPDGATLYVASDTTVKVVEVATGKVVRRFPEVVPPTSTPLPTA
                     PQPPTPGPGTSFPQPTPGVPPSSTPDPDRYYPIQNLELSPSGDRLAVVDYRTIRVW"
BASE COUNT          588 a         1040 c         1010 g          578 t
ORIGIN      
        1 ccgacgccgc catccgcgcc atcaacaccg ccagggtgga ctactacctg atgaagccct
       61 gggacccacc tgacgaacgc ctctacccta tcgtggatga cctgctggac gactggttgg
      121 cggcttaccg ccccgccttc gagggcatac gtatcgtaag ccaccgctgg tcggcccgcg
      181 cccacaacct gaaggacttc ctcgcccgca accagattcc ctaccaggtg ctcgacatag
      241 aggcggacga gcaggcgcag aagctggtgg aactcgcggg cacggggccg gacgccctgc
      301 ccctggtgct cttccccgac ggcacccaca tggtcgatcc tgagccgctc gcggtggcgg
      361 ccaaggtggg catgcgcacc cgcgccgaga ccgagtttta cgacctcata atcgtgggcg
      421 gggggcctgc ggggctcgcg gcggcggtgt acggcggctc ggagggcctg cgcacgttga
      481 tgatcgagcg ggaagcgccg ggcgggcagg cgggcacaag ctcgcgcatc gagaacgacc
      541 tcggcgttcc ggccggcctc agtggcgctg agctggcccg gcgcgctctc actcaggcca
      601 cccgctttgg tgtggaggtg gtgagcttgc aggaggtgtg cgggctgaag gtgaaggacc
      661 cctaccgcac cgtgcagttc accgacggct ccgaagtgac ctgctattcg ctcgtggtcg
      721 ccaccggagt gcagtaccgc aagctcgaag cgccgggcat cgacaggctg ctgggcgcgg
      781 gcgtcttcta cggcgcggcg atgaccgagg cgatggcctg ccaggaccag cacgtctaca
      841 tcgtgggcgg ggctaactcg gcggcgcagg cggccatgta cttcgtcaac tacgccgagg
      901 acgtgaccct gctggtgcgc ggcaactccc tctccgcggg catgtcgcag tacctggtgg
      961 accgcatcga gtcggccccc aacatccacg tgcgcctcaa ttcgagcgtc gcggccgtag
     1021 acggcgacgg caggctggaa accatcacca tagccgactc agccaccggg cagaccgata
     1081 cggtacccgc cggggccatg ttcatcttca taggcgcgtc accgcgcacc gaatggctcg
     1141 acggcgtggt ggagcgcgac aggagcggct tcgtgctctc cggcctcgac ctggtgcgcg
     1201 agggccagag gccgcgaggc tggacccgcg accgcgatcc cttcctgctc gaaaccaacg
     1261 tgcccggtgt gttcgtagcg ggcgacgtgc gccacgactc ggtgaagcgc atcgcctccg
     1321 ccgtgggcga cggcgcgatg gccgtgcagt tcgtacacag gtacctggcg gaagtgagat
     1381 agacgaaatt agaatttggg atttgggatt tcgaatttgc tgtgatatct actaaactcg
     1441 aaatcccaaa ttcccatagg gaggttttgg aatgcaagaa gagatagcgg agtgctcgca
     1501 tttgggcgag gtacaacgtg tgaggcccag cgctaggggt tgcgaggact gcctcaagac
     1561 gggggatgag tgggtgcacc tgcgcgagtg catgagttgc ggctacatcg gttgctgcaa
     1621 cgactccaag aacacccacg ccaccaaaca cttccacgcc tccggtcacc ccatcatcaa
     1681 gtcgttcgag ccgggcgagg actggggttg gtgctacgtg gacgaggtct acttcgagcg
     1741 cctgcccacc cgctaggccc acgcgcaagc gaatatgtgc cttcggagcc ggctgccatc
     1801 tctagcgtta tcgaagttgc ccggtatgtg cggctatgtg gtccgcgctc gccgcaaccc
     1861 aggaggccgg tatgcagggc accttacccg taggggacgc ccctgaccgc aaaccaggtt
     1921 ggccgcgccg cagatacgtt gtggtggggt tggggctgat gttgcttgtg gccgcttccg
     1981 ctattgccat cctggtctgg ttacgccccg ccggccccgc acccctcaag cccttgccgg
     2041 acgtagccga gctagcgtca aactaccggc ttagccagac tctcacggat gcttttgggc
     2101 ctcagtcatg gtctcctgat ggccgctacc ttgtcacgta ttccgcttca acctacggct
     2161 tgcaggtgca tgaccttggt ggccccagcc cgcggacctg gagcgtgccg gccgaagcct
     2221 cgtacagcta ccggagcata gggatagcag aaaaggctat atggtcgccc gatagccgcc
     2281 tgctagcggt ggacgaagct cgtagctcgg ttccttacac taacacggtg gtcgagccta
     2341 atagcgcgct tccttacaca atcacggtgg tagacgcgga aaaaggcacg gtaaggaaca
     2401 cgatccgcgt gctcggccca cctgaattgg cggcgacgcc cacacccgcc gggcgaagcc
     2461 ggacggcggt ggtacaaact ttccaccaac tcgtgggctg ggccgacaac gggcgcagcc
     2521 tggtcacgct cttgttcgta gaaaggcggg aggacgtggc ccgcaatccc ggctactctc
     2581 tttttgaccc cacgcctcaa ccggacgcca cccccgtacc tccttccttc agcgtctact
     2641 tctacgtcca cgatgcggcg acaggcgata tggtgagttc catgcaggta tatttcacca
     2701 cggcctaccc cagcttatac ggcctggcgc tgtcgcccga tggtaatacg cttgcttttg
     2761 caacgacaga caggactccc cggtcggagg ggagcacgag ccaggtcaat cttagtttat
     2821 gggacctgcg cgccggtagg ctcaagcata gagtcgaggg cctgggcgac gaggtgttag
     2881 cgccttttcc gggtatatgg gaccaatttc tcgcctggtc gccggatggt gcgactctct
     2941 atgtggcctc cgacactacc gtaaaggtag ttgaagtggc tacgggcaaa gttgtgcgcc
     3001 gctttcccga ggtagtgcca cccaccagca cgccccttcc aaccgcccct caaccaccga
     3061 ccccagggcc tgggacatcg ttcccacagc caacgcccgg cgtcccaccc agttccacac
     3121 ccgaccccga taggtactat ccgatacaaa acttagaact atcaccctcc ggcgaccgcc
     3181 tggcggttgt cgattaccgg acgatcaggg tgtggg
//
LOCUS       DASRWZ010000280         5766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1119644, whole genome shotgun sequence.
ACCESSION   DASRWZ010000280 DASRWZ010000000
VERSION     DASRWZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119644"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..5755)
                     /locus_tag="VFH60_04360"
     CDS             complement(164..5755)
                     /locus_tag="VFH60_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HET6313044.1"
                     /translation="MTGAIYFSVVLMQRPRWWAAAGAGACLGLAVSSNVSVLPVALVV
                     IAAVVLRAMYRSRTRKLGAEFGDPVGVSPASSEERGHSFGGHLRRGLRYVVLAGVCSI
                     LAFGIGEPYVLWSFDFSTLSNGLEAFLNSNPWSRRIQEEARVQGGIAYVPFTLQYIGT
                     VPVVYQLEQMVYWGIGLVPGLMTVLGFVAGLWHALRRRPAELLLMAAAIPYFFTITPI
                     LTKWMRYMLPLVPIFCLFGAAFLVRGTIWARKRFVAADGVNARSAAGRLVRVQRAVFP
                     ALIVLSIGWAFLWAVAFMNIYTQPHSRIQASEWIHNNVPREATISYEDWDDALPLSLP
                     PIAGRDRSGYPVSIRMTLYDSKPPEAGVEQIKTWLRQLDYIIITSNKQYRSIPRLLWA
                     YPAQTKYYELLFAEKLGFVRVHTTQVSPELLGIKINDQPADESFTVYDHPRVDVFKKV
                     SELTDDQYRMLFSSALNRSSLYNPSRQGSVPDDKGLSYSQPVNTLPQLNDYAWNPLGQ
                     EETQWFAVVLWLALVEVLGLLALPIVFTVFRNVPDRGYAVAKLVALLVVAWGVWIAAS
                     SRAFPFTAWSVLLMVALMAGLSALCWRLGAGRDIRDFFRTKRNLVMAYEVVFLLAFAA
                     VLALRLANPDVWHPIQGGEKPMEIGFLNSVLRSAWMPPLDPFFAGGFINYYYYGYFVV
                     ACLIKVLGIHPAIAFNLAAPLFYALTFTAGMSVVYNLVAWSQKRRGSDSPVSRSGLVF
                     GVGASCLMLVIGNMHGVWQWIMIAFPNLGRGLLDLTRSLNFGEQSMFTQYPSFNWWDA
                     SRIISHTINEFPYWTFLFADLHPHLMDMPFTVLALVFMQNLLFAGAYRKPLLLAYRSN
                     ELSWRRALWLRVSSTLSWLWGPGAVGALNFALMALTLGALLVINSWDFPTFLGLAGGG
                     VLAALLMLGRHGQVDGADEDVPEAQPLPTRISPLERAWIYGVALASVGLLAALALLYY
                     LPFFLNFKAFYTSILPLVDGGQIPNSVDFMHRTTLAEFLVVWGLFVFIAASYLVYRLW
                     HFPWGAAADSLMGIVPGPTAPATRGLSTQQALTLAGDGSSRKARRLVPALSLPGGSTV
                     PVSDAFRFTRGPGEGSLDEAEPEARHTAGPSSDFALAGEATSGKAPVGDGNGSSRGAD
                     SSAGPDANTIGGWLSTASAEEDKTLKVRRRVALGGARSGLDASNGDVAVQNAPGFEIE
                     EVPLAQEAALPARNTAQPGIIPLWAGLGLLGITAALVVLQMVTGQWLLALLVALIGGI
                     AATTLATSRTAANLFCGVLMLGALTVAMGVELVYLADHLQGGLQYRMNTVFKFYMQVW
                     VLFALAGASAIYYMLYGFRERATTTGAARNATRTDALGQPPTTTPASNFTAPEAYRHD
                     HEPKANGSDGAESYNWVVWSLEHADDGVSATSEQSPPAAAPQYAAVETQVLPAETPQE
                     GPLPSINWTPGRLVWLGVLALFLVATLTFTFLGTPDRLAKRFPIPPPVGTLNGLKFMT
                     SATYFGGEPPVQVNLRYDYEAINWLNSNVKGAKVLAELPMEYYRAYGMRAAANTGLSM
                     VVGGLHQDEQRSGSYARLVGERRGDMDAFFTTHDVQVALTILSKYDIDYIYLGQLEQA
                     RAGATGMPKFAQLAGPGVEILREVFRTQQPANVPGTIIYEVVRRPDREVSTLVGAPVA
                     DSGIPGISITPLPTPTATPVPTPPTDDPELQQLINLVAQDPFNRENRLMLVEWYRSHN
                     FPLDAARELQVLVEQDPQNIALRHMLGDAYQQGGQPELALKAWEDARDVDPNNPAGHN
                     KVGIAYLDRKRYDDAVREFQATVDRDPRFVEAYYHMGEAYQLLGERDKALAAYQSVID
                     NAPPGAEGWVDAANRRLAEIP"
BASE COUNT         1141 a         1774 c         1873 g          978 t
ORIGIN      
        1 cgcgggaacg ccagccaggg ttggagtagg cgcattatag tgtaccaaag ttaaggctgt
       61 caagagcttt atacccatca cacccggagg agtggcagcc ccgcgcccgg tgccccgcgt
      121 caaacgcgtc cccgccggcc agttacgcac gggaagctgc ccgctatggt atctctgcaa
      181 gcctccggtt cgccgcatct acccagcctt ccgctcctgg aggcgcgttg tcaatgacgc
      241 tctggtaggc cgcaagcgcc ttgtcccgct cgcccagcaa ctggtacgcc tcgcccatgt
      301 gatagtatgc ttccacgaag cgcgggtccc tatcaactgt cgcctggaac tcgcgtaccg
      361 cgtcatcata ccgcttgcgg tccaggtagg caatgcccac cttgttgtgg ccggccgggt
      421 tgttcgggtc cacgtcgcga gcgtcctccc aggccttgag cgcaagctcc ggctgccccc
      481 cctgctggta ggcatcgcca agcatgtggc ggagggcaat gttctgcggg tcctgctcga
      541 ccagcacctg aagctcgcgg gcggcatcca gcgggaagtt gtgcgagcgg taccactcca
      601 cgagcatgag cctgttttcg cgattgaacg ggtcctgcgc caccaggttg ataagctgct
      661 gcaactcagg gtcgtcggtc ggcggggtcg gtacgggcgt ggcggtgggg gtaggcagag
      721 gtgtgatcga gatgcccggt atgccggagt cggcgacagg cgctcccacc agggtgctaa
      781 cctcgcggtc ggggcggcgg accacttcat agataatcgt tcccggcacg ttggcgggtt
      841 gctgcgtcct gaagacttcg cgcagaattt cgacgcccgg cccggcaagc tgcgcgaact
      901 ttggcatacc tgtggcaccc gctcttgcct gctccaactg gcccagatag atgtagtcga
      961 tgtcgtactt gctgaggatg gtaagcgcca cctgcacgtc gtgggtcgtg aagaaggcgt
     1021 ccatgtctcc acggcgctca cccaccagcc gggcgtagct acctgagcgc tgctcatcct
     1081 ggtgcagccc gcccaccacc atcgacagtc cggtgttggc ggcggccctc atgccgtaag
     1141 cgcggtagta ctccattgga agctcggcga gcacctttgc gcccttgacg ttgctgttga
     1201 gccagttgat agcctcgtag tcgtacctga ggttgacctg gaccggcggc tcgccaccga
     1261 agtatgtcgc agaggtcatg aacttgaggc cgttgagagt acccacagga ggcggtatgg
     1321 ggaaccgctt cgcaaggcgg tcgggcgttc cgaggaacgt aaaggtaagg gtagccacaa
     1381 ggaacaacgc gagcacgccc agccagacca gcctaccagg agtccagttg atgctgggaa
     1441 gaggcccttc ctgtggcgtc tcggcgggca gtacttgagt ctccactgcc gcgtactgcg
     1501 gggcggcggc cggggggctt tgctcgctgg tggcggagac tccgtcgtcg gcatgttcca
     1561 gggaccagac cacccagttg taggattcgg ccccgtcgct gccattggcc tttggctcgt
     1621 ggtcgtggcg gtatgcctca ggagctgtga aattacttgc gggcgtagtt gtcgggggtt
     1681 ggcctagcgc gtcggtgcgg gtggcgttcc ttgccgcccc ggtggtagtt gcgcgctccc
     1741 tgaacccgta gagcatgtaa tagatggcag atgcgcccgc cagggcgaac agcacccaca
     1801 cctgcatgta gaacttgaac acggtgttca ttcggtactg gaggccgcct tgcagatggt
     1861 ctgcgaggta gacgagttcg acacccatag caaccgtcag cgcaccaagc atgaggacgc
     1921 cgcagaagag gttggcggcc gtcctggaag tagcaagcgt agttgctgcg atgccgccga
     1981 tgagggccac caggagcgcg agtagccact ggcccgtcac catctgaagc accacgaggg
     2041 cggccgttat gccaagcagc cccagcccgg cccagagcgg gatgattccg ggttgtgcgg
     2101 tgttgcgggc aggaagggca gcctcttgag cgagtggcac ctcctcgatc tcgaagccgg
     2161 gcgcgttctg cacagctacg tccccgttac tggcgtccag acctgatcgt gcgccgccca
     2221 gcgctacccg ccgccgcacc ttgagcgtct tgtcctcttc ggcgctggcg gtgctcagcc
     2281 acccaccaat ggtgtttgcg tccggcccag cgctgctatc cgccccacgc gaggagccgt
     2341 tgccgtcccc tacgggtgcc ttaccgctgg tcgcttcccc ggcgagggcg aagtccgagg
     2401 aagggcccgc cgtgtgacgt gcttcaggct ccgcttcgtc aaggctgcct tcccctggcc
     2461 ctcgcgtgaa gcggaaggca tctgagaccg gcacggtgct gccaccgggc aaggaaagcg
     2521 ccggtaccag cctcctcgct ttgcgagacg aaccatcccc cgccagggtg agggcctgct
     2581 gggtcgatag gccgcgcgtt gcgggtgctg tgggaccggg tacgatgccc atgagactgt
     2641 ctgcagcagc gccccagggg aaatgccaca gccgatacac caggtacgac gctgcgatga
     2701 agacaaacag cccccaaacg accaggaact cggccagggt cgtccggtgc atgaaatcaa
     2761 cggaattcgg gatttgaccg ccgtcaacca gaggtagtat cgatgtgtag aaagccttga
     2821 agttgaggaa gaacggcaga tagtacaaga gcgcgagcgc agccaacagg ccgacggaag
     2881 ccagcgctac gccgtaaatc cacgcccgct ccaaaggaga aatgcgggtt ggcagcggct
     2941 gcgcctccgg cacatcctcg tccgcgccgt ccacctggcc gtgcctacct aacatcagca
     3001 aggcggcgag tacgccaccc cccgcgagcc ccaggaaggt tgggaagtcc cacgagttga
     3061 tgaccaggag agcgccgaga gttagcgcca tcagggcaaa gttaagcgca cccacggctc
     3121 ccggacccca gagccaggag agcgtgctgc tcacgcgcag ccagagcgcg cgccgccagg
     3181 aaagctcgtt cgagcggtag gcgagcagga gaggcttgcg gtaggcaccg gcgaacagca
     3241 ggttctgcat gaataccagg gccagcacag tgaacggcat gtccatcagg tgcgggtgca
     3301 agtctgcgaa gaggaacgtc cagtagggga actcgttgat ggtgtgcgag attatccggc
     3361 tagcgtccca ccagttgaaa gaagggtact gggtgaacat cgattgctcg ccgaagttga
     3421 ggctacgcgt cagatcgagc aagccgcggc ccaggttggg gaaggcgatc atgatccact
     3481 gccacacgcc gtgcatgttg ccgatgacga gcatcaagca actcgcgccc acgccgaaca
     3541 ccaggcccga acgcgacacg gggctgtcac tgcctcgccg cttctggctc caggcgacca
     3601 ggttgtagac gacgctcatg cccgcggtga aggtgagcgc gtagaagaga ggggccgcga
     3661 ggttgaaagc gatagctggg tgaatgccca gcaccttgat gaggcaggcc acaacgaaat
     3721 agccgtaata gtagtaattt atgaagccgc ccgcgaaaaa cgggtcgaga gggggcatcc
     3781 aggcgctacg caacaccgaa ttgaggaagc cgatctccat cggcttctcg ccgccctgga
     3841 tggggtgcca cacgtcgggg ttggcaaggc gcagggcaag cactgcagcg aaggcgagca
     3901 ggaacacaac ctcatacgcc atgaccaggt tgcgcttggt cctgaagaag tcgcggatat
     3961 ctctgcccgc ccccagccgc cagcatagag ccgagaggcc cgccatcaag gcgaccatga
     4021 gcagcacgct ccaggccgtg aaggggaagg cgcgggagct agccgcgatc cacacgcccc
     4081 aggccacaac gagcaacgca accagcttgg caacggcgta accccggtcg ggtacgttgc
     4141 ggaagacggt aaagacgata ggcagcgcga gcaggccaag cacctccacg agtgccaacc
     4201 acagcacgac ggcgaaccac tgcgtctctt cctgccccag cgggttccag gcgtaatcgt
     4261 tgagctgcgg cagcgtgttt accggctgag aatagcttag ccccttgtcg tcgggcacgc
     4321 tcccctgcct gctcgggttg tacaaagagg agcggttgag cgcgctgctg aagagcatgc
     4381 ggtactggtc gtcggttagc tcgctgacct ttttgaagac gtccacccgg gggtggtcgt
     4441 agacggtgaa gctctcgtct gccggttggt cgttgatctt gatgccgagc agctcaggcg
     4501 aaacttgcgt ggtgtggacc ctgacgaagc ctagcttctc agcgaatagc aactcgtagt
     4561 acttggtctg agcgggatag gcccacagca ggcggggtat cgagcgatat tgcttgttgc
     4621 tggtgatgat aatgtagtcg agttggcgga gccaggtctt tatctgctcc acgccggctt
     4681 cgggcggctt ggagtcgtac agggtcattc tgatcgaaac ggggtagccg ctgcggtccc
     4741 tacccgcgat cggcggcagc gacagaggta gtgcgtcgtc ccagtcctca taggagatag
     4801 tcgcctcacg cggcacgttg ttatgtatcc attccgaggc ctggatgcgc gagtggggct
     4861 gggtgtagat gttcatgaac gccacggccc agagaaaagc ccagcctatg ctcaggacga
     4921 taagggccgg aaacaccgcg cgctgcactc tcacaaggcg gcccgccgcg ctgcgagcat
     4981 tcactccatc ggcggctaca aacctcttac gcgcccagat cgtgcctcgc accaggaatg
     5041 cggccccgaa caggcagaag ataggtacca acgggagcat gtagcgcatc cacttggtga
     5101 ggatcggtgt gatggtaaag aaatagggga tggcagccgc catcagcaga agctccgcgg
     5161 ggcgacggcg cagggcgtgc cacaggcccg ccacgaagcc caggacggtc ataaggcccg
     5221 gcacgaggcc gatgccccag tagaccatct gctcaagctg gtacaccacg ggtaccgtgc
     5281 caatgtactg caaggtaaag ggtacgtaag cgatcccacc ctggacgcgg gcctcttcct
     5341 gtatgcggcg ggaccaggga ttggagttga gaaatgcctc caagccgttg ctcagagtgg
     5401 agaagtcgaa gctccagagc acgtacggct cacctatgcc gaacgcgagt atggagcaga
     5461 cccccgcgag cacaacgtag cgcaacccac ggcgcaagtg gcccccgaag ctgtggcccc
     5521 gctcctcgga cgaggcgggt gacacgccta cggggtcgcc aaattctgcc cccagcttgc
     5581 gagtgcggga gcggtacatc gctcggagta ccacagcggc tatcacgacc agggccacag
     5641 gcagcacgga gacattgctg gacaccgcga ggcccaggca ggcgcccgcc cccgccgccg
     5701 cccaccatcg tggccgttgc ataaggacca cgctgaaata gatcgccccc gtcatgaagg
     5761 cgacca
//
LOCUS       DASRWZ010000281         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_366352, whole genome shotgun sequence.
ACCESSION   DASRWZ010000281 DASRWZ010000000
VERSION     DASRWZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366352"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1752)
                     /gene="menA"
                     /locus_tag="VFH60_04365"
     CDS             complement(58..1752)
                     /gene="menA"
                     /locus_tag="VFH60_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HET6313045.1"
                     /translation="MKNRNRWLSLLLPVLLLLAWELAVRAGVLNRIFFPPPSEVFETL
                     GELAASGRLFRDIGISMTRIVLGFLLGGIPAIVLGLLMGVSPLIRALAQPLAAAIYPI
                     PKIALIPLVLVVLGVNENSKVAIIAISVFFLVVLNVASSVMQIDARYFEVARSFGARQ
                     WDLFWTVALPGSLPGIMNSIKLGMGFALTLIVGVEFVGANEGIGWLIWQSYELYKIDT
                     MLAGLVAVALVGWLVSVLLDELEYLLVPWQAAGLRVKEVRMRQRARIWWGAVRPWSYT
                     AAVIPVLLGASIAAYDGVFRAWPVLLALVGSIAIQGGTNLMNDYFDYRKGADTPGTLS
                     LGGSIVRGDLTPRQVFMGGMLAFGIGIAVGLYLVSLTGPFIFWLGLLSVAAGFFYTAG
                     PFALAYVGLGEVAVFIFMGPVMVIGSYYVQAQTVTLPVVLASIPVGFLVAAILHANNL
                     RDLESDREIGKRTLATLLGRAGANIEYYILVGGTYLSLAVTVLLGIAPWLTLISVVTL
                     PIAVRLMRIVAGETEPKKLHPVLRLTARLHMQFGILLVAGWAAAWLLDSFPIIGFR"
     gene            complement(1749..2549)
                     /locus_tag="VFH60_04370"
     CDS             complement(1749..2549)
                     /locus_tag="VFH60_04370"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313046.1"
                     /translation="MEAPKIAISNLRVVYPGRGRTGPVEALGGVSLDVADGELVCIVG
                     PSGCGKTTLLRVLAGLEQPTSGSAHIRNDDPKRPSQAMVFQSAGIFPWMTVEQNVAYG
                     LQLRGVPRPERVATARRWISEIGLDRFAKTYPAQLSGGMQQRVGLARAFAYDPEVLLM
                     DEPFGALDAQTRLLLQQTLLQQWESDDKTVIFVTHSIEEALTLGDRVFVMSARPGLLL
                     HEVQVPFERPRDAVALRTDARFSALFAEIWGVLRDEVERTRLAEEVHV"
BASE COUNT          542 a          912 c          762 g          402 t
ORIGIN      
        1 ggacgaagag cataaggata ttaaagtaga ctagctcgcg tgtaacaggg tatgcaacta
       61 tctgaagccg ataatcggga agctatcgag cagccatgcc gccgcccagc ccgctaccag
      121 caggataccg aactgcatat gcagccgtgc ggtaaggcgc agcacagggt gcagcttctt
      181 cggctcggtc tcacccgcaa cgatacgcat gaggcgcaca gcaatgggca gggtcacaac
      241 gctgatcaac gtcagccagg gggcgatgcc cagcagcacg gtgacggcca gcgacaaata
      301 ggtgccgccc acaaggatat aatattcgat gttggccccg gcccttccca gcagcgtcgc
      361 gagggtgcgc ttgccgatct cgcggtcgct ctcaaggtcg cgcaggttgt tggcatggag
      421 tatggccgcc accaggaagc ctacgggtat agacgccagc actacgggca gcgtgaccgt
      481 ctgcgcctgc acgtaatacg agccgatgac catcacaggc cccatgaaga taaagaccgc
      541 tacctccccc agcccgacgt aggccagcgc gaaaggccct gccgtgtaga agaagcccgc
      601 cgccacgctc aacagcccca gccagaagat gaacggcccc gtcaggctca ccaggtagag
      661 gccgactgct attccgatgc cgaacgccag cataccaccc ataaatacct gccggggagt
      721 gaggtcgccc cgcacgatgg agccgcccag gctcaacgtg ccgggagtgt ccgcgccctt
      781 ccggtaatcg aagtagtcgt tcatcaggtt ggtgccaccc tggatggcta tcgagcctac
      841 cagggccagc aagacgggcc aggctctgaa caccccatcg taagccgcga tgctcgcgcc
      901 cagcagcacg gggatgaccg ccgccgtgta gctccacggg cggaccgcgc cccaccagat
      961 acgcgcacgc tgcctcatgc ggacctcctt cacccgcaag cccgccgcct gccagggcac
     1021 cagcaggtac tcaagctcgt caagcagcac cgacacgagc cagcccacca gcgccaccgc
     1081 caccagcccc gccagcatgg tgtcaatctt ataaagctcg taggactgcc atatgagcca
     1141 gcctatcccc tcgttagcac ccacgaactc cacgcctacg atcagggtca gggcgaagcc
     1201 catacccagc ttgatactgt tcatgatgcc gggcaggctg ccgggcagcg ccaccgtcca
     1261 gaagaggtcc cactggcgag cgccgaagct gcgcgccacc tcaaagtacc tggcgtctat
     1321 ctgcataacg ctcgacgcga cgttcagcac gaccaggaaa aagacgctga tggcgataat
     1381 agccaccttg ctgttctcgt ttacccccag caccaccagc accaggggta tcagggcgat
     1441 cttggggata gggtagatcg cggcggccag cggctgcgcc agcgcccgga tgagcgggct
     1501 gacgcccatc agcaggccca gcacaatcgc ggggatgccg cccaacagga accccagcac
     1561 gatacgcgtc atgctgatgc cgatgtcgcg gaacagccgc ccgctcgccg ccaactcccc
     1621 cagcgtctcg aacacctccg agggcggcgg gaagaatatg cggttgagca ctccggccct
     1681 gaccgccaac tcccacgcca gcaggagcaa caccggcagg aggagcgaga gccacctgtt
     1741 gcggtttttc atacgtggac ttcctcggcc agccgggtgc gttcgacctc gtcccgcagc
     1801 acgccccaaa tctcggcgaa gagcgcgctg aagcgtgcat cggtgcggag cgcgacggca
     1861 tcgcggggcc gctcgaacgg cacttgcacc tcgtggagca gcaacccagg ccgtgccgac
     1921 atcacaaaca cccggtcccc cagcgtcagg gcttcctcga tgctgtgggt cacgaagatg
     1981 accgtcttgt cgtcgctctc ccactgctgc aagagcgtct gttggagcag gaggcgcgtc
     2041 tgagcgtcga gcgcgccgaa cggctcgtcc atcagcagca cctcagggtc gtatgcgaaa
     2101 gcgcgggcca gcccgacacg ctgctgcata ccgcccgaaa gctgcgcggg gtacgtcttg
     2161 gcgaagcggt ccaaccctat ctccgaaatc cacctgcggg cggtagcaac ccgctccggc
     2221 ctgggcacgc ctctcaattg gaggccatac gccacgtttt gctctaccgt catccagggg
     2281 aagatgcccg cgctctggaa gaccatcgcc tgcgaaggac gtttggggtc gtcgttgcgg
     2341 atgtgcgcac tgccgctagt gggttgctca aggcccgcca gcacccgcag cagggtcgtc
     2401 ttgccgcaac cactcggccc gacgatgcac acaagctccc cgtccgccac gtcgagcgac
     2461 accccaccca acgcctccac aggcccggtg cgtccccggc caggatagac cacgcgcagg
     2521 ttgctgattg ctatcttcgg cgcttccatc tgcttattgc ttaccttacc tcttgtccaa
     2581 ctccgccgcg acctcggacg caagctgcgt atccacga
//
LOCUS       DASRWZ010000282         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_366464, whole genome shotgun sequence.
ACCESSION   DASRWZ010000282 DASRWZ010000000
VERSION     DASRWZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366464"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /gene="aspS"
                     /locus_tag="VFH60_04375"
     CDS             complement(<1..1162)
                     /gene="aspS"
                     /locus_tag="VFH60_04375"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HET6313047.1"
                     /translation="MERIWTSELPNHVDERVQLAGWLHRLRRHSKVSFLILRDAKGLA
                     QVVVEEPSLVERLGELYSESVISVEGVVSAEAQAPNGVEIREPKVIIVSAASSPPPID
                     LFRPSLQAQLPTILDYAPLALRHPRHRAIFPVSAASMLGFRETLHSLGFVEVQTPKIV
                     ASATEGGANVFSIEYFGRTAYLAQSPQFYKQMLVGVFERVFEVGPVFRAEPHDTPRHL
                     NEYVSLDLEFGFVQDHSTVMSLLAEVLRGMLAAIQREAPGALSLLGITLPEVPATIPS
                     IHFAEAQKLMHEATGEDTSHEPDLSPSQERWLGEWARREHNSDFLFVVGYPMVKRPFY
                     THPDPERPLYSNSFDLLFRGLELVTGGQRLHRYADYMAVMAAREMDPTPFAEY"
BASE COUNT          439 a          680 c          669 g          395 t
ORIGIN      
        1 ggtactccgc gaagggcgtg gggtccatct cccgcgccgc catcaccgcc atgtagtccg
       61 cgtagcggtg cagccgctgt ccgccagtca ccagctccag cccccggaac agcaggtcga
      121 agctgttgga gtagagcggc cgctcagggt cggggtgcgt gtagaagggg cgttttacca
      181 tcgggtagcc gaccacgaac aggaagtcgg aattgtgctc ccgccgcgcc cactctccca
      241 gccaacgttc ctgcgagggc gacaggtcgg gttcgtgcga agtgtcctcc cccgtggcct
      301 cgtgcatgag cttctgcgcc tccgcgaagt ggatagatgg tatcgtagct ggcacttcag
      361 gtagggtgat gccgagcaac gacaacgccc ccggcgcttc ccgctgtatc gccgccagca
      421 tgccccgcaa gacctccgcc agcagcgaca tgacggtgga atggtcctgg acgaagccga
      481 actcaaggtc cagcgacacg tactcgttga ggtggcgggg cgtgtcgtgc ggctcggccc
      541 tgaacaccgg gcctacctcg aacacgcgct cgaacacccc caccagcatc tgcttgtaga
      601 actgcgggct ttgtgccagg taagcggtcc tgccgaaata ctcgatgctg aacacgttag
      661 ccccgccctc ggtagccgaa gccactatct tgggggtctg tacctccacg aagccgagag
      721 aatgcaacgt ctcgcggaac cccagcatgg aggccgccga caccgggaag atcgcgcggt
      781 gtcgcgggtg ccgcagggcc aggggcgcgt agtcgagtat cgtgggtagt tgggcttgca
      841 gcgaggggcg aaacaggtct atgggtggcg ggctgctcgc cgccgacaca attatgacct
      901 tcggctcgcg tatctccacg ccgttgggag cctgcgcctc cgctgaaacc acaccttcga
      961 ccgagattac cgactcgctg tacagttccc ccagccgctc caccagggac ggctcctcaa
     1021 ccaccacctg agccagtccc ttagcgtcgc gcagtatgag gaagctgact ttgctgtgcc
     1081 ttctcaggcg gtgcagccag ccggcaagtt gcacccgctc atccacgtga ttgggtagct
     1141 cgctcgtcca tatccgttcc atagtgacac ctcctaacgc attaaatgca gaaccccatc
     1201 gccatgcgga cgatggggct ttcgtggtgc caccgcattt caccgcgcca cgcctccctg
     1261 tcctgtcgag gcatgtagcg gccttttgcc ctgtaacggg ggcgagccgt cggggcttac
     1321 ttgggcagtt gctgcccgtt cttccccgcg ctccggagtg tcttcacagt gcccgggggc
     1381 aaccatccca gcaacggttg ctctctgcgc cccggcgcta cctgctactc gtctccatcc
     1441 acgctttgta agtgcagatt ctaacggaga atacaggcca ggtcaatccg ccgctggtag
     1501 taatcgcggt acaacttaag agacggaacc gccaagacgc caaggacgcc aagagcgcgc
     1561 caagattaag tacggttcac cgcagaggcg cagagagcct gccctgagcg cagccgaagg
     1621 gacgcagaga ttacgcagag ccaagggctt atttaggaaa gggcaagggc agcagtggac
     1681 tgtacgctgc tatccttgcc agaacatcct caattcttct tggcgtcctt ggcgtcttgg
     1741 cggtttcatc tcctcacaaa aaccccttga caaccctccc cggcttccgc tatagtcact
     1801 ttacacaaac ctatacccga aagcgtggat gggaagagta gcggcccagg tggcagacag
     1861 agaacgggcg gcaggtggaa agcccgcgtg caaacggacc gcgaaaaaga ccctgagttg
     1921 caggaagaaa tcgcactgcg aaagtagaac cttgccggtg gcccccgtta cagggcatga
     1981 gtacaggcga ctcgaaaagg ccggttgtcg agaggcatac ggcgaacgaa ggtggtacca
     2041 cgagagcata gctcttcgtc ctttgcggga tggagggcgt ttttgtttaa gtggcaccgg
     2101 gtaccggagg tcgaggcacg cccgtactcg gtgaggccgg acgctgagag gcgtccggcg
     2161 aagaaaggtg gtaccacgga agc
//
LOCUS       DASRWZ010000283         1668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1325426, whole genome shotgun sequence.
ACCESSION   DASRWZ010000283 DASRWZ010000000
VERSION     DASRWZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325426"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1318)
                     /locus_tag="VFH60_04380"
     CDS             complement(83..1318)
                     /locus_tag="VFH60_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010999320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313048.1"
                     /translation="MPRKPRIALYSHDAMGLGHMRRNLLIAQTLACPPLDSVVLMLAG
                     AGEAGTFGMPPGVDCVTLPALRKNGNGSYRSRSLDLPFDELISMRAHIIRAALQAFDP
                     DVLIVDKVPRGIGGELNQSLDYLHARGHTRCVLGLRDVLDEPEAVHREWGRDGNEDAL
                     RTYYDAVWVYGDPAVYDMVREYNLSPAIATKVRYTGYLDQCKRLGAVGEDADRQFHAL
                     SLPNSRLVLCLVGGGQDGDRLAEAFAQVNLPPGAYGVIVTGPYMPADVQRRLHALASD
                     HARLRVLDFVPEPAVLLARADRVIAMGGYNTTCEILSFGKPALIVPRVKPRLEQWIRA
                     ERLRDMGLLDVLHPDGVTPQALADWLARDIRPLDSVRDRLDMKGLSRLPNLLRDVLDT
                     RRQPASCRIHHRGYEYAAY"
BASE COUNT          308 a          452 c          583 g          325 t
ORIGIN      
        1 ttcatgggcg agtatctcgt ttactatgaa cgtttctgag tagcggggat agcgtttgag
       61 cacgtagcct acccgcttgt tatcaatagg cagcatattc gtatcctcta tggtgaatgc
      121 ggcatgaggc gggctgtcgc cgcgtgtcca gcacatctct gagcaggttg gggaggcggg
      181 agaggccttt catatcgagg cggtcgcgca ccgagtcgag gggccggatg tcacgcgcca
      241 gccagtcggc gagggcctgg ggggtcacgc cgtccgggtg gagcacgtcc agcaggccca
      301 tgtcgcgcag acgctcggcc ctgatccact gttcgaggcg gggcttgacc ctgggcacga
      361 tgagggcagg cttgccgaag gagagtatct cgcaggtcgt attgtagccg cccatcgcga
      421 taacgcggtc ggcgcgggcc agcaagacgg caggctcagg cacgaaatcg agcacccgca
      481 gcctggcatg gtcggacgca agggcatgca atcggcgctg cacgtcggcg ggcatgtaag
      541 gccccgtgac gatcacgccg taagcgccgg gcggcaagtt gacctgggcg aaggcttcgg
      601 ccagccggtc gccgtcctgc cccccgccaa ccaggcagag cacgagcctg ctgttgggga
      661 ggctcagggc gtggaactgc cggtcggcat cctccccgac ggcacccaac cgcttgcact
      721 ggtcgaggta cccggtatag cgtaccttcg tggcgattgc gggggagagg ttgtattcgc
      781 gcaccatatc gtagactgct gggtcgccgt acacccatac ggcgtcgtag taggttctca
      841 gcgcgtcttc gttaccatct cgcccccatt ccctgtggac ggcctccggt tcatcgagaa
      901 cgtcgcgtaa gcccaggacg caacgggtgt ggccgcgagc gtgtagataa tcgagggact
      961 ggtttagctc gccgcctatg ccgcgcggca ccttgtcaac tatgagcacg tcggggtcga
     1021 atgcctgtag tgcggcgcga ataatgtgcg ctcgcatgga gataagttcg tcaaaaggca
     1081 ggtcgaggct gcgggagcgg taagagccgt tgccgttctt tctgagggcg ggcagggtta
     1141 cacagtctac gccgggtggc atgccgaaag tgcccgcttc gcccgcgccc gcaagcatga
     1201 gcaccaccga atcgagcggt gggcatgcaa gagtctgcgc gatgaggagg ttgcggcgca
     1261 tgtggcccag gcccatcgca tcgtgcgaat agagggctat gcggggcttg cgcggcaact
     1321 cacgcccgtt tgtgcgagag aaaatagatt tcggaatgag gttgcgagca gcatttgctc
     1381 tcaggggagg ggcattatct ttagtacgct gtggaggagt gcgtatacct tttgtcctgt
     1441 ccctacctcg gccgttccag gctagtgagg atttcatggc gggtccttgc cagaactcga
     1501 aagttggact taagagtctc ttcaggaatc gcggctgctg agcgggaggt cctcttcggg
     1561 gaggaagtcg ggttcttgaa ggaggaacgg ttgcttggag gggcaaccac cggtgtggga
     1621 ggagctttgg ctacaagtag gaggccggga ggggaagagt aagctagt
//
LOCUS       DASRWZ010000284         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_297952, whole genome shotgun sequence.
ACCESSION   DASRWZ010000284 DASRWZ010000000
VERSION     DASRWZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297952"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VFH60_04385"
     CDS             <1..677
                     /locus_tag="VFH60_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET6313049.1"
                     /translation="LARNRRVSEDTAIGVLFAGMFALGIVLISTVEGYTADLASFLFG
                     NVLGVSVEDLVASVVIGALVLVLLVLFHKELVLVSFDNDMAEAAGLPVWLLNLGLLLL
                     IAVTIVVSLRAVGNILVVAMLVTPAAAARLWTDRLRVMMGLSALFGALAGVLGLLVSY
                     HANLAAGGSIVLAVTLWFGVSLLVAPRHGYLGRFLRPASKGKLAGPEGVLAQSASPSE
                     GHGHVH"
     gene            728..1192
                     /locus_tag="VFH60_04390"
     CDS             728..1192
                     /locus_tag="VFH60_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET6313050.1"
                     /translation="MPGVEAVLERLNHEGYRLTGPRRLVLVEVLQRTTPFTSAELWDS
                     IQQTAPTIGRATVFRTLDLLTRLGVVQRIHRDAEAGRCHAYLACDEQHHHHLICNSCG
                     NVTDFHEDRALEALVREVERRTAFQVEGHRLELVGTCPACRNDSQSHIGDTC"
     gene            1186..>2014
                     /locus_tag="VFH60_04395"
     CDS             1186..>2014
                     /locus_tag="VFH60_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HET6313051.1"
                     /translation="MLTNSHRAKLLRALAVLSLMLLAACDAGTPGSSTSTADSPGSGL
                     NVVATTTQIRSMAEAVLGDRGTVRSILPPGADAHDFEPKPNIVQAISESDVVLKNGIG
                     LDDWVDKLIENAGGVRPLITVTVGIPLRAGDEEGEHGQSDPHVWLNVTNAITMTANIR
                     DALIQADPANEAAYQANAGTYITRLRELDRYIKDQVDTIPPEDRKMVTNHDALGYYID
                     AYGLTFVGSIIPSMSTGAQPSAQDVAELIRKIKEAKVKAIFLESRVNPALARQVGSDT
                     "
BASE COUNT          338 a          647 c          657 g          372 t
ORIGIN      
        1 cgctggctcg caaccggcga gtgtctgagg acacggcgat tggcgtgctg ttcgcgggca
       61 tgttcgccct gggcatcgtg ctcattagca ccgtcgaggg ctacaccgcc gacctcgctt
      121 cgttcctgtt cggcaacgtg ctgggcgtga gcgtggaaga cctggtcgcc agcgtggtta
      181 tcggcgcgct ggtgctggtg ctgctggtcc tgttccacaa ggagcttgtg ctggtgtcgt
      241 tcgacaacga catggccgag gccgccgggc tgcccgtctg gctgttgaac ctggggctgc
      301 tcctcttgat cgcggtgacg attgtggtgt ctttgagggc ggtgggcaac atcctggtgg
      361 tggcgatgct ggtcacgccc gccgccgccg ctcgcctgtg gaccgaccgc ctgcgcgtga
      421 tgatgggcct gagcgcgctg ttcggggcgc tggcgggggt gctgggcctg ctggtgagct
      481 accacgccaa cctggcggcg ggcggcagca tcgtgctggc ggtcaccctc tggttcggcg
      541 tttcgttgct ggtggctccc aggcatggct acctgggccg cttcttgcgc ccggcgagca
      601 agggcaaatt ggccggaccg gagggggtgc tggcgcagtc cgcctctcct tccgagggcc
      661 acggccacgt tcattgaggc atcgcaagag gccacttact tagctggcct caaattagga
      721 gagtttcatg cccggcgtcg aagcagtgct cgaaaggttg aatcacgaag gctaccggct
      781 caccggcccg cgccgcctgg tgctggtgga ggtgttgcag cgcaccacgc cctttacgtc
      841 tgccgagttg tgggactcca ttcagcagac cgctcccacg ataggccgcg ctacagtctt
      901 ccgcacgctc gacctgctca ccaggctggg cgtggtacag cgcatccacc gcgacgccga
      961 ggccgggcgc tgccacgcct atctcgcctg cgacgagcag caccaccatc acctgatctg
     1021 taactcctgc ggcaacgtta ccgatttcca cgaggaccgc gcgctcgaag cgttggtgcg
     1081 cgaggtggag aggcgtactg ccttccaggt cgaggggcac cggctggagc ttgtgggcac
     1141 ttgccccgcc tgccgcaatg attcgcagtc acacataggc gacacatgct aactaactcc
     1201 caccgggcta aactgctgcg cgctttagct gtgttgtccc tcatgctgct ggcagcttgc
     1261 gatgcgggca cgccgggtag ctccacttcg accgcggact ccccaggctc cgggctgaac
     1321 gtagtggcta ccaccacgca gattcgctca atggccgagg ccgtactggg cgaccgtggc
     1381 accgtgcgct cgatattgcc gccgggggcc gatgcgcacg acttcgagcc caagccgaat
     1441 atcgtgcagg ccattagtga gagcgacgtg gtgctcaaaa atggcatcgg cctggacgat
     1501 tgggtggaca aactgatcga gaacgcgggc ggcgtgcggc ccctcatcac ggtgacggtg
     1561 ggcatccccc tccgcgccgg tgacgaggaa ggcgagcacg gccagtccga tccgcacgtc
     1621 tggctcaacg tcaccaacgc gattacgatg acggccaaca tccgcgacgc cctcatacag
     1681 gccgacccgg ccaacgaagc tgcctaccag gccaacgccg gtacttacat cacgcggctg
     1741 cgcgagctgg accggtacat caaggaccag gttgacacta ttccccccga agaccgcaag
     1801 atggtgacca accacgatgc cctcggctac tatatcgatg cctacgggct gacttttgtc
     1861 ggctctatca tcccctcgat gtccaccggg gcgcagccgt cggcgcagga cgtggctgag
     1921 ctaatacgca agatcaagga agctaaagtg aaggccatct tcctggagag ccgcgtaaac
     1981 ccggccctgg cgcggcaggt aggcagcgac acgg
//
LOCUS       DASRWZ010000285         3508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1257207, whole genome shotgun sequence.
ACCESSION   DASRWZ010000285 DASRWZ010000000
VERSION     DASRWZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257207"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFH60_04400"
     CDS             <1..320
                     /locus_tag="VFH60_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313052.1"
                     /translation="GAILHYTNGKWEEVPNPDGVVISDFYMLSPRDGWAVGSGGAILH
                     YDGDKWTLVDTGIRGVHLESLDMVSPDEGWAIGREDRSANTAGVILHYQHGKWSVYSR
                     GSR"
     gene            complement(499..2055)
                     /gene="rpoD"
                     /locus_tag="VFH60_04405"
     CDS             complement(499..2055)
                     /gene="rpoD"
                     /locus_tag="VFH60_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET6313053.1"
                     /translation="MLQAHSLDDEFDPVTSMQATYRKGKPSHLQMVPLDVPEESDLHL
                     AIVPAIDDSVDTVDIPGIGLPGLPGMSGIPGITDDDGDVLEPEVTQEQIDRLLDLEGV
                     STDDPVRMYLREIGRVSLLSADAEVSLAKRIEKGEALRTKVAEIEERYGFTPPGEVIC
                     LEIYEGLIARWPALEAIYKGHYDTRQLPESRREVLQAIAPVESIDVNLLRKVGMRDGK
                     PPEKLADEVRTILLTTAMLPELLQETFDMGQWPDGEELALILLDCADTLDRHWSLVLE
                     DARAARAHLAEANLRLVVSVAKKYAGRGMTLLDLVQEGNIGLLKAVEKFRYSKGYKFS
                     TYATWWIRQAITRAIADQARTIRIQVQRVETINRVLKTSRRLMQELSREPSPEELGKA
                     VGMPADRVREILRISQEPISLETPIGEEEDSHLGDFIEDHKAIAPADAATHNMLKAQI
                     VTVLGSLTKRERKVLQLRFGLEDGRSRTLEEVGREFGVTRERIRQIETKALRKLRHPS
                     RSKKLKDYME"
     gene            complement(2491..>3508)
                     /locus_tag="VFH60_04410"
     CDS             complement(2491..>3508)
                     /locus_tag="VFH60_04410"
                     /inference="COORDINATES: protein motif:HMM:NF021895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313054.1"
                     /translation="DMAALKGSEVLQEHADKIAIPVRGERGLMGVERRSQREIRVMQL
                     PRGMDPDELLLAEGGPARWDELREGALPLVDHVIGVVAAKHDLNSAHGNSEAVVEMAL
                     YVREIGDPVQRAHYMQRVASVLRVPEDAVREAISRATPQKRDGRKARERDEGRTAEQP
                     VVQSPRSSNSLPTTVDMITSAEEHLVSLVLKYPQVTWMAGAPLPEDFMRLENRLIFET
                     VSRAAAAESNKSTPVAEEIRAAAREQLDEALLAHFDRIAVREEPELFRFALPYELETR
                     LKRLRQHNDRMWLHQCQVMLQEAQETDDKDTIGKLLPLLTASFSRFRHYQPKPSTVFR
                     DSRD"
BASE COUNT          559 a         1200 c         1020 g          729 t
ORIGIN      
        1 ccggcgcgat attgcactac accaacggca agtgggagga ggtacccaac cccgacgggg
       61 tagtcatatc ggacttctac atgctatcgc cccgtgacgg ctgggccgtc ggcagcggtg
      121 gcgctatcct ccactacgac ggcgacaaat ggaccctggt agacaccggc atcaggggcg
      181 tacacctcga aagcctcgac atggtctctc ccgatgaagg ctgggcgata ggccgcgaag
      241 accgctcggc caacacggca ggggtaatcc tccactacca gcacggcaag tggagtgtct
      301 acagcagggg cagccgctaa gagacggaac cgccaagacg ccaaggacgc caagagcgcg
      361 ccagattaag ggtgggttgg agagtacaaa aaggaccaga gtacatgcga cgtactctgg
      421 tcctttttct cagaacacat acttaatctt ggcgtgatcc ttggcgttct tggcgtcttg
      481 gcggtttcgt ctccgttcct actccatgta gtccttaagc ttcttggagc gggaggggtg
      541 gcgcagcttc cgtaacgcct tggtctctat ctggcggatg cgctcgcggg tgacgccgaa
      601 ctcgcggccc acctcttcca gcgtgcggct cctgccgtct tccaggccga agcgtagctg
      661 tagcaccttg cgctcccgct tggtgagtga gccgagcacc gtcactatct gcgccttgag
      721 catgttatgg gtggccgcat cggcaggcgc tatcgccttg tggtcctcga tgaagtcgcc
      781 caggtggctg tcctcttcct cgccaatcgg cgtctccagc gaaatcggct cctggctgat
      841 gcgcagtatc tcgcgcaccc ggtcggcggg catgcccacc gccttgccaa gctcctcagg
      901 agaaggctcg cggctgagtt cctgcatcag gcggcgggag gtcttgagca cgcggttgat
      961 cgtctccacc ctctgcacct ggatgcggat cgtgcgggcc tggtccgcga tggcgcgggt
     1021 gatcgcctgg cgtatccacc acgtcgcgta cgtggagaac ttgtagccct tgctgtagcg
     1081 gaacttctcc accgccttga gcagtccgat gttgccttcc tgcaccaggt cgagcagcgt
     1141 catgccccgc cccgcgtact tcttggcgac gctcaccacc agccgcaggt tggcctcggc
     1201 caggtgggcg cgggcggccc tggcgtcttc cagcaccagg ctccagtgcc tgtcgagcgt
     1261 gtcggcgcag tcgagcaaga tgagggcaag ctcctccccg tcgggccact gacccatgtc
     1321 gaaggtctct tgcaggagtt cgggcagcat ggccgtggtg agcagtatcg tgcgcacctc
     1381 atcggccagc ttctcaggcg gcttgccgtc gcgcatgccc accttgcgca gcaggttgac
     1441 gtcgatgctc tccaccgggg cgatagcctg caacacctcg cgccgcgact cgggtagctg
     1501 cctggtgtcg tagtgcccct tgtagatagc ttccaacgcg ggccaccgcg cgatcagccc
     1561 ctcgtagatt tccaggcaaa ttacctcgcc gggcggggtg aagccgtaac gctcctcgat
     1621 ctccgcgacc ttggtgcgca gcgcctcgcc cttctctatc cgcttggcga gggacacctc
     1681 ggcgtccgcg ctgagcagcg acacccgtcc gatctcgcgc aggtacatgc gcaccgggtc
     1741 gtcggtgctc accccctcca ggtcgagcag cctgtcaatc tgctcctgcg tgacctccgg
     1801 ctccagcacg tccccatcgt cgtcggtgat gccggggatg cccgacatcc ctggtagacc
     1861 tgggaggcca atgccgggga tatctacggt gtctacggag tcgtcgatgg ccggtacgat
     1921 agcgagatgc aggtcgcttt cctctggtac gtccagcggc accatttgga ggtggctggg
     1981 cttgcccttg cggtaggttg cttgcatcga tgtcacagga tcgaactcgt cgtccaggct
     2041 gtgggcttgc agcatatctt gccttcctct cctacgtcct caaaactcag actacactct
     2101 ctacacttcg ctcgaactct cttttgcccc ggcccgcggg cctccccgcc tgccggacct
     2161 tacccccgtg gccctcactc acgccccgtg tccgcatgag agccgatagc ttgttcgccc
     2221 tcaattcacc cccctcagtt tcgaccttaa gctactgatt ttgtgttcta caaacacttt
     2281 gcatggtgcc cactgcaatc cggaatctaa actacccact taagcagttc ctataggaag
     2341 atagattctc cttcagtaca acacctctcc tccatcacac tgacatagcc tgccgtgtct
     2401 ctactcctac cccaaacaat cctaaacttt gcgcgtcctt tgcgtccttt gcgtctttgc
     2461 gttttcgtcc gtctcgttgg actgcacccc tcagtcccgg ctgtcgcgaa agacggtgct
     2521 gggcttcggc tggtagtgcc ggaagcgcga gaaagacgcg gtcagcaacg gcaggagctt
     2581 gcctatcgtg tctttgtcgt ccgtctcctg ggcctcctgc aacatcacct ggcactggtg
     2641 cagccacatc cggtcgttgt gctggcgcag ccgcttcaat ctcgtctcaa gctcgtaggg
     2701 gagggcgaag cggaatagct ccggctcctc gcgcacagct atgcggtcga aatgggccag
     2761 caacgcctcg tccaactgct cgcgggcggc tgcgcgtatc tcctcggcca cgggcgtgct
     2821 cttgttgctt tccgccgctg ccgccctgga gactgtctcg aatatcagcc ggttctcaag
     2881 ccgcatgaag tcctccggca gcggcgcgcc cgccatccag gtcacctgtg ggtacttgag
     2941 caccaggctc acgaggtgct cctcggcgga ggtaatcatg tccaccgtgg tcggcaggct
     3001 gttcgaggac ctgggactct gcactacggg ctgttcggcg gtgcgccctt cgtcccgctc
     3061 cctcgccttg cgcccgtcgc gcttctgcgg ggtggccctg cttatcgcct cgcgcaccgc
     3121 gtcctcaggc acccgtagca ccgaggccac acgctgcatg tagtgggcgc gctgcaccgg
     3181 gtcgccaatc tcgcgcacgt agagggccat ctcgactaca gcctcgctgt tgccgtgcgc
     3241 gctgttcagg tcgtgcttgg ccgccaccac gccgattacg tggtccacca gcggcaaagc
     3301 cccctcccgc agctcgtccc agcgcgccgg gccgccctcc gccagcagca actcgtcagg
     3361 gtccatgccc ctcggcaact gcatcacgcg gatctcacgc tgcgaccgtc gctccacgcc
     3421 catcagcccg cgctcgcccc gcaccgggat ggcgatcttg tccgcgtgct cctgtagcac
     3481 ctcggagcct ttaagggccg ccatgtcg
//
LOCUS       DASRWZ010000286         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_503412, whole genome shotgun sequence.
ACCESSION   DASRWZ010000286 DASRWZ010000000
VERSION     DASRWZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503412"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFH60_04415"
     CDS             complement(<1..247)
                     /locus_tag="VFH60_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313055.1"
                     /translation="MFEHVRAVFLDAGLTLIYSELSLPTLCYEVGREYGLRVSLPALE
                     AAVPRATAFLHHTQRGDPDLWSSDDRVQRLWAGFYTAV"
     gene            complement(255..461)
                     /locus_tag="VFH60_04420"
     CDS             complement(255..461)
                     /locus_tag="VFH60_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313056.1"
                     /translation="MGAVVLVAALYGVYKLEQLDLSQTNAAPAGAPDPVPLSRRIQRF
                     RTGPLPKAPVGDPVPQEEHSGSVR"
     gene            complement(465..1424)
                     /locus_tag="VFH60_04425"
     CDS             complement(465..1424)
                     /locus_tag="VFH60_04425"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HET6313057.1"
                     /translation="MQQLLDPQPNISIGPLLLIFLLAMLFTFVATPLARRVAHRYSVV
                     DAPSARKIHANPVPLLGGAAIYLAVIVSLVLFGHLQYVQQTASILIGATLMSMLGVWD
                     DKWGLRPLAKLVGQVIAASLLFYSGISVEFLHNDVLNYGATVLWVVTITNALNLMDNM
                     DGLAGGVAAVASVFFFLIATLTDQLLVAPMAAVLVGACLGFLYYNFNPAYIFMGDTGS
                     LFLGFMLAAVGIKLRFPANFDIVTWMVPVLVLGVPLFDTTLVTLSRIRRGIPISRGGK
                     DHLSHRLVALGYTKREAVMVLYLIQGMLGVAALVVMQADLLEG"
     gene            complement(1443..>2349)
                     /locus_tag="VFH60_04430"
     CDS             complement(1443..>2349)
                     /locus_tag="VFH60_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313058.1"
                     /translation="YANLGRLYLWWADPAGGNDPSKNELAVREMENAATRSPGNAQIR
                     DELAVAYARNNQFAKAIETLNYSQHELDPTFARTPFIRSQLTGERAGQIRGLLDQGQP
                     LPTDGETDYGKLMLDVGRAYSETIALDPLLVIDNNFTTRIDALLEATKPYTKTNTTLP
                     ETTVTNILTNTVVIALENQLVQREQDVTAYLRERGAYTGTDQFVSSELLTQLFQDPNW
                     AGTQAPDRPPGWLDAEMARRTHVAAMLNYGIAYVYSKLERQGYPTEGLVRATALEPYG
                     QFNMPNTQQQQQPPQTTTETQPTQP"
BASE COUNT          414 a          679 c          844 g          412 t
ORIGIN      
        1 agacggcggt gtagaacccg gcccagagcc tttgcacccg gtcgtcggag gaccacaggt
       61 cggggtcgcc gcgctgcgtg tggtgcagga aggccgtggc gcgaggcacc gctgcctcta
      121 acgcgggcag cgacacacgt aggccgtact cccgccccac ttcgtagcag agggtaggca
      181 gcgaaagctc ggagtaaatc agggtcaacc ccgcgtcgag gaagaccgcg cggacgtgct
      241 cgaacatggg gcggtcagcg gacagagccg gaatgctcct cctggggcac ggggtcaccc
      301 accggggctt tagggagggg gccggtcctg aagcgttgaa tgcggcggct gagaggcacc
      361 gggtcgggcg cgcccgcggg tgcggcgttg gtctgcgata ggtctagctg ctccagcttg
      421 tacaccccgt agagagcggc cacgagcacc accgcgccca cgacctatcc ctcaagcagg
      481 tcggcttgca tcaccacgag cgcggcgacc cctagcatgc cctgtatcag gtagagcacc
      541 atgaccgctt cgcgcttggt gtagccgagg gccaccaggc gatgggagag gtggtctttg
      601 ccgccccggc tgatggggat gccccggcgg atgcgcgaga gggtgaccag ggtggtgtcg
      661 aacagcggca cgccgagcac gagcacgggc accatccagg tgacgatgtc gaagttggcc
      721 gggaagcgca gcttgatgcc cacggcggcc agcatgaagc ccaggaagag cgagccggtg
      781 tcgcccatga agatataggc ggggttgaaa ttgtagtaga ggaagccgag acacgccccc
      841 accagcaccg ccgccatagg agccaccagc agttggtcgg tcagcgtggc gatgaggaag
      901 aagaagaccg aggccaccgc cgccacgccg cccgcaagcc cgtccatgtt gtccatgagg
      961 ttgagcgcgt tggtgatggt taccacccac agcacggtcg ccccgtagtt gagcacgtcg
     1021 ttgtggagga actccaccga gatgccggag tagaagagga gggaagcggc tatcacctgc
     1081 cccaccagct tggctaaggg gcgcaagccc cacttgtcgt cccacacgcc cagcatggac
     1141 atgagggtcg cgccgatgag gatggaggcg gtctgctgga cgtactgcaa gtggccgaac
     1201 agcaccaggc tcacgatgac cgccaggtag atggcggccc cacccaacag aggcacgggg
     1261 ttagcgtgta tcttacgggc cgagggcgcg tctaccacgc tgtacctgtg cgccacccgc
     1321 cgggcaaggg gcgtggccac gaaggtgaac agcatggcca gcaagaagat gaggagcagg
     1381 gggccgatgc tgatattcgg ttgtgggtcg agcagttgct gcatggtcct ggaacgtggc
     1441 cgctagggct gcgtcggctg cgtctccgtg gtcgtttgcg gaggctgctg ttgctgttgc
     1501 gtgttgggca tattgaactg gccgtaaggc tccagcgcgg tggccctgac gaggccctcg
     1561 gtgggatagc cctggcgttc cagcttgcta tagacgtacg cgataccgta gttcagcatc
     1621 gcggcgacat gggtgcgcct cgccatctcg gcgtcgagcc agccgggggg cctatccggg
     1681 gcttgcgtgc cggcccagtt ggggtcctgg aagagttgcg ttaagagttc gctagacacg
     1741 aactggtcgg tgccagtata cgcgccccgc tcgcgcaggt aggccgttac gtcctgctct
     1801 ctctggacaa gctggttctc cagggcgatt acgaccgtgt tggtgaggat gttggtcact
     1861 gtggtttcgg gcagcgtggt gttggtcttc gtgtatggct tggtggcttc cagcagagca
     1921 tcgattcggg tggtgaagtt gttatctatc accagcagag ggtcgagggc aatcgtctcg
     1981 gagtaagcgc ggcccacgtc gagcatcagc ttgccgtaat cggtctcgcc atcggtgggc
     2041 aggggctggc cctggtctaa caggccgcga atctgtccgg cacgctcccc ggtcagttgg
     2101 gagcggatga agggggtgcg ggcgaaggtc gggtcgagtt cgtgctgaga gtaattgagc
     2161 gtctctatcg ccttggcgaa ctggttgtta cgcgcgtagg ccacggcaag ctcgtccctg
     2221 atctgggcgt taccgggcga gcgcgtggct gcgttctcca tctcgcgcac ggccagctcg
     2281 ttcttcgagg ggtcgttgcc gccggctggg tcggcccacc agaggtagag gcgacccagg
     2341 ttggcgtag
//
LOCUS       DASRWZ010000287         4787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_298137, whole genome shotgun sequence.
ACCESSION   DASRWZ010000287 DASRWZ010000000
VERSION     DASRWZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298137"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFH60_04435"
     CDS             complement(<1..213)
                     /locus_tag="VFH60_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET6313059.1"
                     /translation="MRIVNLTPEDEGLVRQVAALLVEAFREHWPEAWPDIDSALAEVR
                     ESFAEGRISRVAVGEGGEARGWIGGVP"
     gene            complement(541..1149)
                     /locus_tag="VFH60_04440"
     CDS             complement(541..1149)
                     /locus_tag="VFH60_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313060.1"
                     /translation="MQTNIRTGNVQASPAIQLYTLAGMSALLYVVLSLAAMALTDWNN
                     PAATSTPALVLGVLGNIAVLPVVVAVYQHLRPASGALSSVAVISGVLALIGAAVGSVV
                     GYDSALGMASGLSASFGMLLFFGLSGYLALTSKLLPAGWAYLSIIVGLLAGAAGIISG
                     SAGSSSDMTGLAWTAFNVSTLVWALWTAVEFLRRGRATSAGE"
     gene            complement(1205..1483)
                     /locus_tag="VFH60_04445"
     CDS             complement(1205..1483)
                     /locus_tag="VFH60_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313061.1"
                     /translation="MPQDAEYEADKIQPQPQAQAQAQHAPQSTSHLFLVRLWAESDSE
                     GEAVWCGKVQHVTKGRPNQFRDWPALIDLFKTMLPDAERVRHHTALEE"
     gene            complement(1679..2359)
                     /locus_tag="VFH60_04450"
     CDS             complement(1679..2359)
                     /locus_tag="VFH60_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313062.1"
                     /translation="MKTKAQGLFRTLVVAFAATALLPAAVFAQPGPPAGVLIDQPPEL
                     AAARAKFENMTAEAATAAGYMSTHECVSSPFGGMGIHYIHPQLFESQIRSGEMDPQNP
                     PVLLVDANSRVVGLEWETTQNTPQPTFLDQKVPLQPGHPGMEEEHYMVHIYFKPNNKL
                     LLGAFDPDLKCPAGGAGGPPEGAAGAAPVGMPQTGNTAGELLPLSLALAAGLALLAAG
                     ATLRKRKA"
     gene            2544..>4787
                     /locus_tag="VFH60_04455"
     CDS             2544..>4787
                     /locus_tag="VFH60_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6313063.1"
                     /translation="MRSEVRTFSQWVKEGRKALDLTQEDLAERVGCSIWTIRKIEMGE
                     RKPSRQVVELLADALRVPADRRPAFISLARTGSGTGELVVHPPGDSPAQTPNNLLASP
                     TALIGREAEVAKARAWLLSEDVRLLTLVGPPGIGKTRLALEVAGALLDEFADGVFFVP
                     LAQVSEPPLVASTVAQVFGLKETGEEPIQAKLVQHLQNERMLLVLDNFEQVVGAANLV
                     GDLLARCPRLKVLVTSREALRLRGERQFSVPPLALPQEEQPLPVETLASYPAVRLFVE
                     RAQAVSGFRLTEENAANVATICARLDGLPLAIELVAARSKLLPPKAILARLMRSEGRT
                     SLRLAAGGARDVPPRHQTLRAAIEWSYDLLEAGEQKLFARFGVFVGGCSIAAAEAVCN
                     ARGDLAVDALEGVESLLDKSLVRQSGQEEEDVEVDWEPRFEMLETIREYARERLRESG
                     EEEQTGQWHAEYYLALAEATEAELGGSRQGEWLDRLEQEHDNLRAALQWSLVDAESPG
                     DGPSNDEKLELALRLCGALGSFWEMHSHLSEGRKWFDLALERGHSVPPAARAKALSGA
                     GTLARYQGDFASARRLFDESLALRRELGDKKGIAASLNNLGTIAWNTGDYATARLLFA
                     ESLAVRQELGNTPAIAASLSNLAAVTRHLGEPDEARRYQEESLALMRELGNKRGIATA
                     LNNLGGAAWGQGDYATARLLFEESLALARELGHKSGVADVLCNLGEVLTAQGEYTGAY
                     PLPVASLK"
BASE COUNT          901 a         1503 c         1523 g          860 t
ORIGIN      
        1 cgggacaccg ccaatccaac cccgcgcctc tccaccctcg cccaccgcca cgcggctgat
       61 gcgtccctcg gcaaacgact cgcgtacctc cgccagggcc gagtcgatgt caggccaggc
      121 ctccggccag tgctctcgga acgcctcaac gagcagggcg gctacctgcc ggaccagccc
      181 ctcatcctct ggcgtgagat tgacaatgcg catggcacct cccacaggca ttattgtgaa
      241 acgcgatgta ctctggagag attataagcc acgatgtgga gaggatagtt ccgttgtcca
      301 gcgcacagcg taccaccgag ggccagtcgg ttgagaacag gccctcggtg gtacgccgcg
      361 tatcccttta tgtcacggag ttccattcta ccagtttctc ggccccagtg cctggcaggc
      421 cgcgtagcac ccggcagtgc tctcgtcggg gactgtatgg gagacccctc acgtggctta
      481 gctaagacgt cttaccccgg tagcggaagc tgaagtcctc cctcctgatg tgtcgcgtca
      541 ctactcgcct gcggatgtgg cgcggcccct acgtaggaac tccactgcgg tccaaagcgc
      601 ccataccaag gtagacacgt tgaaggcggt ccatgctagg ccggtcatat cgctactcga
      661 accggcgcta cccgatataa ttccggcagc accggccaat agtccgacga ttatgctcag
      721 gtaggcccag ccggccggca agagcttgct cgtgagagcc aggtagcccg acaggccgaa
      781 gaacaggagc attccgaaac tggccgatag accgctagcc atgcccaggg cgctatcata
      841 gcccactacg ctgccgacag cggccccgat cagcgccagc acaccgctta taaccgcaac
      901 gctgctgagc gccccggaag ccgggcgcag gtgctggtag actgccacca caactggaag
      961 caccgcgata ttgccgagca cgcccaggac cagggcgggg gtgctggtcg ccgccgggtt
     1021 gttccagtcg gtcaaagcca tcgctgcgag gctcagtact acgtagagca gcgcgctcat
     1081 accggccagt gtgtacaact ggatcgccgg tgaagcctgc acgtttccgg tcctaatatt
     1141 ggtctgcatg tttttcctcc ctatctgcta acgatcatca tcaggtaaca acgtccgggt
     1201 cctgttattc ttccagggca gtgtggtgcc ggacccgctc ggcatcgggc agcatggtct
     1261 tgaagaggtc tatcaacgcg ggccagtccc tgaactggtt cggccttccc ttcgtaacgt
     1321 gctgcacttt gccgcaccac accgcctcgc cctcgctgtc actctctgcc cacagccgca
     1381 ccaggaacag atgtgatgtg ctctgcgggg catgctgtgc ctgtgcctgc gcctgcggct
     1441 gtggctgtat tttgtcagcc tcgtattctg cgtcctgggg catttgcatg cctctccttc
     1501 tcccggcggt agtgcatttg cggggtcccc ggtaagctgg gctagagttt ggcgctgcgt
     1561 gcgtaccact ccaggtgtgc atggtttcca gcgtaccggg gccgcctcac tatcacctat
     1621 gcgtcagcta cgtcggccgc agcagacaac ccacctcggt cgaaccgtag ttcggacctc
     1681 aggctttccg cttgcggagc gtagcccctg ccgcgagcaa ggccaatccc gctgctagcg
     1741 ccaggctcag gggcagtagt tcccctgcgg tgttgccggt ctgcggcatg cctacaggag
     1801 cagcgccggc agcaccctcc ggtggaccgc ctgccccacc ggcagggcat ttaaggtcgg
     1861 gatcgaaggc accgagcagc agcttgttgt tgggcttgaa gtagatgtgg accatgtagt
     1921 gctcttcctc catgcctggg tgccccggct gaagcggcac cttttggtcc aggaacgtgg
     1981 gttggggagt gttctgggtc gtctcccatt ccaggcccac cacacggcta ttagcgtcca
     2041 ccagcagcac agggggattt tgcgggtcca tctccccgct cctgatctgg ctctcaaata
     2101 gttgcgggtg gatgtaatgg atgcccatcc ccccgaaagg tgagctgacg cactcgtgtg
     2161 ttgacatgta ccccgccgcc gtagcagcct cagcggtcat gttctcaaac ttggcccgtg
     2221 ccgcggccag ttcgggcggc tggtcaataa gcactccggc aggcggtccg ggctgtgcga
     2281 agacggcggc gggcagaagc gccgttgcgg caaatgctac taccagtgtg cggaaaagtc
     2341 cctgtgcctt ggttttcatc ggtgttgcct cctcaatctt ttctttcaga tgcttggtct
     2401 agaatgccac tcaaaagtga taacctgtcc acctacattg tggccgagga taggctataa
     2461 tgagtcgcac catcattagc acaaaactag cacaagcggt gtggccacga aggcggcaga
     2521 aggcggcagg cacggagggc gctttgcgaa gcgaggtacg cacgttcagc caatgggtca
     2581 aggaagggcg caaggccctg gacctcacgc aggaggacct ggcagagcgg gtaggttgct
     2641 cgatatggac catccgcaag atcgagatgg gcgagcgcaa accctctcga caggtagtgg
     2701 agttgctcgc cgatgccctg cgcgtgcccg cggaccggcg ccccgccttc atcagccttg
     2761 ctcgcacagg ctccggaacg ggcgagctgg tcgtgcaccc tccaggcgat agcccggcac
     2821 agactcccaa caatttgctc gcttctccca ctgctttgat cggaagggaa gccgaagtag
     2881 caaaagcgcg cgcctggctc ctgagcgagg acgtgcgcct cctcacattg gtcggccctc
     2941 caggcatcgg taagacgcgc ctcgccctgg aagttgcagg agcgctgctg gacgagttcg
     3001 cggatggcgt gttcttcgtg cctttggccc aggtgagcga gccacccctc gtagcttcca
     3061 ccgtcgccca ggtattcggc ctgaaagaga ccggtgaaga acctatccag gccaagctcg
     3121 tacaacacct gcagaacgag cgcatgctac tcgtgctcga caacttcgag caggtagtgg
     3181 gcgcggcgaa cctcgtgggg gacctcctgg caagatgtcc ccggctgaag gtgcttgtaa
     3241 cgagccggga ggcgcttcgc ctgcgcggtg agcggcagtt cagcgtgccg cccctggcgc
     3301 taccccagga ggagcaaccc ctccccgtag agactctggc gagttacccg gcggtgcgct
     3361 tgttcgtgga gcgcgcacag gctgtgagcg gcttcaggct cacggaagag aacgccgcga
     3421 acgtggctac catttgcgcc cggctggatg gcctgcccct cgcgatcgag ctggtcgcgg
     3481 cgcgcagtaa attactaccc ccgaaagcga tcctcgccag gcttatgcgc tcggagggcc
     3541 gcacctctct acggctggcg gcaggcgggg cgagagacgt acccccccgg caccagacgc
     3601 tgcgggccgc catcgagtgg agctacgacc tgctcgaagc aggcgagcaa aagctatttg
     3661 cgcgttttgg cgtgttcgta gggggttgca gcatcgcggc ggccgaggcg gtgtgcaacg
     3721 ccaggggcga cctggcggta gatgcgctgg aaggggtgga atcgctgctc gacaagagcc
     3781 tggtacgaca gagcggccag gaagaagagg acgttgaggt agattgggaa ccccgcttcg
     3841 aaatgctcga aacaatccgc gaatacgcga gggagaggtt gcgggagagc ggggaggagg
     3901 aacaaacggg gcaatggcac gccgagtatt acctggccct ggctgaagcc accgaagcgg
     3961 agttgggcgg ctcgcggcag ggcgagtggc tcgaccgcct cgagcaagaa cacgacaact
     4021 tgcgcgcggc gctgcaatgg tccctggtgg acgccgagtc ccccggcgac ggcccgtcca
     4081 acgacgaaaa gctggagcta gccctccgcc tgtgtggggc gctcggtagc ttttgggaga
     4141 tgcacagcca cctcagcgaa gggcgcaagt ggttcgacct ggcgctggag aggggccatt
     4201 ccgtgccccc cgcggccagg gccaaggcgc tgagcggggc gggcacactc gcccgctacc
     4261 agggagactt cgcaagcgca cgacggctgt ttgatgaaag cctggccttg cggcgggaac
     4321 tgggcgataa gaaaggtata gccgcctccc taaacaacct gggaacgatt gcctggaaca
     4381 ccggcgatta cgctacggcc cggctgctgt ttgccgaaag cctggcagta agacaggaac
     4441 tggggaacac accggctatc gcggcttccc tgagcaacct ggccgccgtg accaggcacc
     4501 tgggcgaacc cgacgaggcg cgccgctacc aggaagagag cctggctctg atgcgcgagc
     4561 taggcaacaa gcgcggtatc gctacggctc tgaacaacct cggaggtgcg gcctgggggc
     4621 agggggacta cgctacggcc cgcctgctgt ttgaggagag cctggctctc gcccgggagc
     4681 ttgggcacaa aagcggcgtg gctgacgtcc tatgcaacct gggcgaagtg ctaacggctc
     4741 agggtgaata cacaggggcc tacccgctcc ccgtggcgag cctcaag
//
LOCUS       DASRWZ010000288         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_846558, whole genome shotgun sequence.
ACCESSION   DASRWZ010000288 DASRWZ010000000
VERSION     DASRWZ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846558"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFH60_04460"
     CDS             complement(<1..395)
                     /locus_tag="VFH60_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313064.1"
                     /translation="MLGRWFYRSRQFFTALGVGARLDREDVKEARRVLGPSLYRLFAA
                     MPDPYKRHGLYVYRKVREAGGHDESLLQAALLHDTGKYDPASGRYVTVPHRVAVVLLG
                     AVPFGRPALDWLSRHREARGLTGYVLYPF"
     gene            complement(415..1074)
                     /locus_tag="VFH60_04465"
     CDS             complement(415..1074)
                     /locus_tag="VFH60_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6313065.1"
                     /translation="MFGLQFDPVTFFGFMLGFVIGTTFHEFMHAYSAFLLGDDTAARQ
                     GRITLNPAAHFDPFGFIMGILLALGIGGIAWGRPVPVNPYALKGGRRGMAIVAVAGPV
                     SNLVFASALVLFYHGASGMLPPQVKGVIENMILINVLLFCFNLIPIPPLDGFNILVGI
                     LPNYWNVVLEPIRRYALPILIGFVFLVPYVGNALRFDLNPITEILRPVAGGILRIFGL
                     T"
     gene            complement(1089..1520)
                     /locus_tag="VFH60_04470"
     CDS             complement(1089..1520)
                     /locus_tag="VFH60_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313066.1"
                     /translation="MAELASNSMDSSRMTGKPTLMAIYPRRYEVAAILDSIGRVGYPL
                     NDVSVYYRVKGTDHVVDATTGQVAAGQSINQEEVTPEVLNNAETVVLLHPGADVLSAV
                     TEALIAVGEPNILYEGETGVEGRLGGYTRLDETHNAEVPGS"
     gene            complement(1557..>2163)
                     /locus_tag="VFH60_04475"
     CDS             complement(1557..>2163)
                     /locus_tag="VFH60_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6313067.1"
                     /translation="GTRYKGAEPLRHLVYLTISTGVGGGVITDGKLLVGSTGMAAELG
                     HIVIDMGGPRCGCGNLGCLEACASGTALAREGYLVVKSRRPTKMAELVKGVAEDVTAE
                     TVFQAAREGDHEAMLLVQREGMLVGVGVVNCIHAFNPQLIVLGGGVSGAKELLFDPVR
                     ATVDDRIMPAYKGTFQIVPAALGGDTGALGAVAAVLVELKQ"
BASE COUNT          414 a          740 c          630 g          379 t
ORIGIN      
        1 tagaacgggt agagcacgta gccggtaagc cccctggcct ctctatgccg cgacagccaa
       61 tcgagggcgg gacggccaaa cggtaccgcc cccagcagca ccaccgctac cctgtgcggc
      121 accgtaacgt accggccact cgcggggtcg tacttgcccg tatcgtgcag caacgccgcc
      181 tgcaatagac tctcatcgtg cccgcccgcc tcgcgcactt tcctgtaaac gtacaatcca
      241 tgccgtttat acgggtccgg catggccgcg aacagcctgt aaagggaggg gccgagcacg
      301 cgccgggcct ctttcacatc ctcccgatcc agcctggcac ccacgcccaa cgcggtaaag
      361 aactgcctcg acctgtaaaa ccacctgcct agcactccgg ggcgcaccgg actttcacgt
      421 gaggccgaat atacggagaa tgccacccgc gacggggcgc agtatctccg ttatcgggtt
      481 caggtcgaac cgtagcgcgt tgccgacgta cggcacgaga aacacaaagc caatcaaaat
      541 gggcagcgcg taccgcctga ttggctcaag gacgacgttc cagtagttag ggaggatacc
      601 aaccaggatg ttgaagccgt ccaggggagg gatgggtatg agattgaagc agaagagcag
      661 gacgtttatc aggatcatgt tctctattac gcccttcacc tgcgggggaa gcatacctga
      721 agcgccgtgg tagaacagga caagcgcgct agcgaacacc aggttggaca ccggccccgc
      781 cacggccacg attgccatac cgcgcctgcc ccccttgagc gcgtagggat tcacgggcac
      841 cgggcggccc caggctatcc cgccgatgcc gagcgcgagc aggatgccca tgatgaagcc
      901 gaacgggtcg aagtgcgcgg cgggattgag ggtgatgcgg ccctggcgag cagccgtgtc
      961 gtcgcctagc aagaacgccg agtaggcatg catgaactcg tgaaaggtgg tgcctatcac
     1021 gaagcccagc atgaagccga agaacgttac cgggtcgaac tgtagtccaa acataagttt
     1081 atgcgcttct aactgccggg tacctcggca ttgtgcgttt cgtccagccg cgtgtaacca
     1141 cccaggcggc cttccacgcc ggtctcgcct tcgtagagga tgttcggctc tccgaccgcg
     1201 atgagggcct ccgtcaccgc cgacaagacg tccgcgccgg ggtgaagcaa cacgaccgtt
     1261 tcggcgttgt tcagcacttc cggggtgact tcctcctggt tgatcgattg ccccgcagcc
     1321 acctgccccg tggtggcatc caccacgtga tcggtgccct tcacccggta gtacaccgat
     1381 acgtcgttta aaggataacc caccctgccg atgctgtcca atatggctgc cacctcataa
     1441 cgtcgcgggt aaatcgccat gagcgtcggc ttgcccgtca tgcggctgct gtccatactg
     1501 ttgctcgcca attctgccat gctggcaccc ccctgttatc ctgcacgccc tcggtgctac
     1561 tgcttgagct cgaccagaac cgcggctacg gcaccgagcg cgcccgtatc gccacccagg
     1621 gcggcgggca cgatctggaa ggtgcctttg taggcgggca ttatgcggtc gtccacggtg
     1681 gctcgcaccg ggtcgaacag tagctctttg gcacccgaca ccccgccgcc cagcacgatg
     1741 agctgcgggt tgaaggcgtg tatgcagttg accaccccca cgcccaccag cataccctct
     1801 cgctgcacca gcaacatggc ttcgtggtcg ccctcgcgtg cggcctggaa caccgtctcg
     1861 gcggtcacgt cctccgccac gcccttgacc agctctgcca tcttcgtcgg gcggcgcgac
     1921 ttgacgacca agtagccctc ccgcgccaac gccgtaccgg aggcgcaggc ctcaaggcag
     1981 ccgaggttgc cgcacccgca gcgcggcccg cccatatcta tcacgatatg cccaagctcg
     2041 gcggccatgc cggtgctgcc caccagcagc ttgccgtccg tgattactcc gcccccaacc
     2101 cccgtgctga tggtcaggta gaccaggtgc ctcagcggct cggccccctt gtaccgggtg
     2161 ccc
//
LOCUS       DASRWZ010000289         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_915094, whole genome shotgun sequence.
ACCESSION   DASRWZ010000289 DASRWZ010000000
VERSION     DASRWZ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915094"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..588
                     /locus_tag="VFH60_04480"
     CDS             232..588
                     /locus_tag="VFH60_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6313068.1"
                     /translation="MEVVNLAEKFGKFEDYWSPKIVGELNDFHVKLVKVKGEFVWHHH
                     EVEDELFLVVKGRLLIRLRDRDLWLNEGEFAIIPHGVEHMPYAEEETHILLLEPAATV
                     NTGNVQNERTAVVERI"
     gene            873..1727
                     /locus_tag="VFH60_04485"
     CDS             873..1727
                     /locus_tag="VFH60_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET6313069.1"
                     /translation="MITDADPQPQTPGAAGGGPDETAPAEHSTNGAFEVHLEVFEGPL
                     DLLLKLIERRQLDISKVALADVTDAFLSYMSAHPDMPPAPMASFVWVASKLLWIKSQA
                     LLPRPPATRTEDDEEDPGDELVRRLEAYKRVQEAAKWLRNRETAGLRSYERPATLEAL
                     PIASRPRLSPEDMGNGLDGVTLAKLVKMVQKRMQLGMPAIPTGTATKGHVITVAEKVS
                     HLRKRLAPLKRGERLALGMEFAEAAMRSRTELVVLFLAVLEVIRRKMAAAEQDEAFGE
                     IWLMRAEE"
     gene            1896..2945
                     /locus_tag="VFH60_04490"
     CDS             1896..2945
                     /locus_tag="VFH60_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET6313070.1"
                     /translation="MGIDVYGNLLAGLEDRLHRIEHPPVITLTTDFGTHDGYVGAMRG
                     VIHTLLPDAKVVDISHDITPQQIQEGAFILYRAYRYFPASAVHIAVVDPGVGSSRRSI
                     VLSTRHGTFVGPDNGIFTYVLRAEQAPVPNPDVDRMPWIGGMWGVAPNWAGELELEEA
                     DGSAADPVAGEVLEDDPYAHPGDLPHAYHLDNPDYWLANVSNTFHGRDVFAPVAAHLA
                     SGAHPDRMATPVSIDTLVTLPDAAPRVQRSRQGTTIVGQVIHIDRFGNIITNLPDRLL
                     EPLLEATGATALIEIAGYSIKGLGQSYADVRRGQALALIGSERLLEVAVRNASAAQRM
                     KVSVGDQVRIVVQGS"
BASE COUNT          640 a          952 c         1019 g          548 t
ORIGIN      
        1 ccacccaggc ctgggggcac gcagagcaat cgagcacggc gcatcgcccg gcctggcacg
       61 tctgatagcc aaccaccagc accccaaccc cacggacgag accctgcgcc gcctgcaagc
      121 tgccgacgac agttgaacaa atttaggatt taggaattag gatttaggat ttggtaggta
      181 tccaagcaaa tcctaaatcc taattcctaa attaccagga ggggatggat tgtggaagtt
      241 gtgaacctgg cggagaagtt tgggaagttt gaggactatt ggagccccaa gattgtgggg
      301 gagttgaacg acttccatgt gaagttggtc aaggtgaagg gggagttcgt ctggcaccac
      361 cacgaggtgg aggacgagtt gttcctggtg gtgaaggggc ggctgctcat acggctgcgc
      421 gaccgcgacc tctggctcaa cgagggggag ttcgccatca taccgcacgg tgtagagcac
      481 atgccctacg ccgaggagga gacccacatc ctgctgcttg agcctgccgc cacggtgaac
      541 accggcaacg tgcagaacga gcgcaccgcc gtagtcgagc gcatataatc accgccttgc
      601 gggccggctt tccgtaccat cgctgaccat tctacctctg gtcctcctgg gtcaccgccc
      661 gtcagtctgt attcccctct tgtcatcctg agcgtagcga aggatcaggt gggtaggcga
      721 gagtcttcaa cgttggggat gccggcggcc agcggcacag gggtgccgta acattgagag
      781 tcaaacatgg gcggctgatc cttcgctacg ctcaggatga caaggcgggc ctttggtcgc
      841 cggaggctga atagaacctc tatcaagcta gcatgattac agacgccgat ccacaaccgc
      901 agacgcccgg tgcagcaggt ggaggccccg acgagaccgc gcccgccgag cactcgacca
      961 acggggcctt cgaggtgcac ctggaggtgt tcgaggggcc gctcgacctg ctgctcaagc
     1021 tgatcgagcg caggcagctt gacatcagca aagtggccct ggccgacgtg accgacgcct
     1081 tcctctccta catgtcggca caccccgata tgccgcccgc gcccatggcc tctttcgtgt
     1141 gggtggcgag caagctcctg tggatcaagt cgcaggctct gctgccccgc ccacccgcta
     1201 cgcgcacgga ggacgacgag gaggacccag gcgacgagct ggtgcgccgg ttggaggcct
     1261 acaagcgcgt acaggaagcc gccaaatggc tgcgaaaccg cgaaacggct ggacttcgtt
     1321 cctacgagcg gcccgccact ctggaagctc tgcccattgc ctcccggccc aggctttcgc
     1381 ctgaggatat gggcaacggc ctggatggtg tgacgttagc gaagctggtg aagatggtcc
     1441 agaagcggat gcagctgggc atgcccgcga tacctaccgg caccgccacc aaggggcatg
     1501 ttattaccgt ggccgagaag gtcagccatc tgcgcaagcg gttggcccca ctcaagcgcg
     1561 gggagcggct ggccctgggc atggagtttg cggaggcagc catgcgctcg cgcactgagc
     1621 ttgtggtgct tttcctcgcc gtgctggagg taatacgccg caaaatggcc gcagccgagc
     1681 aggacgaagc tttcggggag atttggctta tgagggcgga ggagtagtca gtggtcggtg
     1741 gtcggtggtc agtggtcagt gaagagacga aaacgcaaag acgcaaagag cgcaaagaac
     1801 acgcaaaggt tatatgttgt ttggatggcg ggttagttgt cactgaccac tgaccactga
     1861 ccactgacca ctcgtccacc acgggggaca acgtcatggg catcgacgta tacggaaacc
     1921 tgctggcggg gcttgaggac cgccttcaca ggatagagca cccgcccgtt atcacgctca
     1981 ccaccgactt tggcacccac gacggctacg tgggcgcgat gcggggcgtg atacatacgc
     2041 tgctgcccga cgcgaaggtg gtcgatatca gccacgacat caccccgcag cagatacagg
     2101 agggcgcttt catcctctac cgcgcgtacc ggtacttccc ggcgagcgcg gtgcatatcg
     2161 cggtggtgga ccccggcgtt ggtagctccc ggcgctctat cgtgctgagc acccggcacg
     2221 gcaccttcgt tggacccgac aacggcatct tcacctacgt gctcagggcg gagcaagccc
     2281 cggtgccgaa cccggatgtg gacaggatgc cctggatagg cggcatgtgg ggcgtcgctc
     2341 ccaactgggc gggcgaattg gagcttgagg aagctgatgg ctcggccgcg gacccggtgg
     2401 cgggtgaagt gctggaggac gacccgtatg cccacccagg cgatttgccc cacgcctacc
     2461 acctggacaa ccccgactac tggctggcga acgtgagcaa caccttccac ggccgcgacg
     2521 tgttcgcccc cgttgccgcc cacctcgcca gcggcgcgca ccccgaccgc atggctaccc
     2581 cggtcagtat tgacacgctg gtgacgctgc cggacgccgc gcccagggtg cagaggtcgc
     2641 gccagggcac taccatcgtg gggcaggtga tacacatcga ccgtttcggc aacatcatca
     2701 ccaacctgcc cgacaggctg ctcgaaccgc tgctggaggc caccggcgcg accgccctga
     2761 tcgaaatcgc gggctacagc ataaagggcc tggggcagtc ttacgcggat gtgcggcggg
     2821 ggcaggcgct ggccctcatc ggctcggagc gcctgctgga ggtggccgtc cgcaacgcca
     2881 gcgccgccca gcgcatgaag gtaagcgtgg gcgaccaggt gcggattgtg gtgcaggggt
     2941 cgtaggggtg cgggtgggca tcaactcaag cttcgtatga tgccctgtgg gggtgccaag
     3001 catgacgccc tgttgctaaa gccacgggct gggaagtagc gaagtctcct tcggagactc
     3061 aaagctcaat cgaaagcgaa agggtggaat acgagcacct aagcacctac tttgtcattt
     3121 cgaacgcagt gagaaatctc gtccttcagc acccaaata
//
LOCUS       DASRWZ010000290         4208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_23993,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000290 DASRWZ010000000
VERSION     DASRWZ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23993"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="VFH60_04495"
     CDS             complement(<1..1697)
                     /locus_tag="VFH60_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6313071.1"
                     /translation="MQSTPVEQPNTADSDRKPATLGRSFIRTLRGQLILRTLLPLTLL
                     VVAFAVVGQVGYTQVSESLAKSRDAELARIQAERVGDHLSLAVQVLQQVASSPVLLDP
                     KDDTGIFYELKKESVIRQFDMVQVTGPDGKLIASNLAAIAGDVLNKRAYESLSKGIGP
                     ATVIIPTELRDGRPASLVTLPFIDNRGGYGGMVEGVIFYGSPKLGRPFGLQRAATANG
                     GAGHSISYLVASDGRVLWHPDQKLVGTMLPSLALNDLTTSEPSAIITPLNGEQHIIGY
                     APLNLALLFPRADLDLTWLRWYVVTQERWADIVAPLNSLLSGLLVLALVTMLLAVLLV
                     ARSAGTLTRPVAKLVAASQALSAGRLRHRLQVSGPVEIEELARQFNSMARQLQASYSE
                     LEAKVTERTGELAAANAELERRLVESVTMQRVTADLAGTAGLQEILQTIASSVSEMLS
                     TESCLVFLPSESRPDELEAAIGWNSPKLASANHLPVDQSLSGVAFRTGQVQISNNAAS
                     DERVNHTLRQEMGAHSVLAAPMVSRGHAIGVVTAVNKKQGDFTHDDLRVMSVLANQAA
                     "
     gene            complement(1699..2103)
                     /locus_tag="VFH60_04500"
     CDS             complement(1699..2103)
                     /locus_tag="VFH60_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313072.1"
                     /translation="MLDLLVTIHSVFRWVVLLTAVLAIAVALVSATGTRRWGVVGDRS
                     SLFFTIAMDVQFLIGLLVWVLGQRWQGDVGLGWLHPLAMLGAVALAHVGRARADLGRH
                     ATSTDKGRQGAIFFTASLLVVLIAIPLNSWPV"
     gene            complement(2308..3288)
                     /locus_tag="VFH60_04505"
     CDS             complement(2308..3288)
                     /locus_tag="VFH60_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET6313073.1"
                     /translation="MRNFDYTKAGTLEAALSAVGQGDGAALLAGGTDLLPLMKDEIVV
                     PRALVDIGGLTGLSYVEDRGGTLHLGAMSTLSDIAAHPLVGERYPALAQACGLAATPQ
                     LRNMSTIGGNLLQQPRCWYYRGQHNCWLKGGEVCFARNGENEQHAIFLNSPGESRCVS
                     VHPSDPAVALLLFDAVVRMQTPQGETEAPLRDLYVVPTPERRNSFAMPPGAVITEIVL
                     SPFASGRSVYLKAMPRASWAFALVSVGLYVRVAAGSIEEARLTLGGVAPIPLRFPEIE
                     EQLVGQETDRLDADSLADSLVATATPLSQNGYKVPLLRGLFKQALSQVIE"
     gene            complement(3288..>4208)
                     /locus_tag="VFH60_04510"
     CDS             complement(3288..>4208)
                     /locus_tag="VFH60_04510"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET6313074.1"
                     /translation="GPPAHALVRLNSDGTVEVICGTQDIGTGIRTALSQVAAEELGVP
                     LDVVRFRLGDTQKGPFAPASWGSITVPSVGPAVRGAAEDAKQQLLEIASYFFEVPASQ
                     LQVRDGHITVEGREEGKRSIGDLLSEVGDYMIVGKGFRGPNPAEPLRTWGAQIADVAV
                     DLATGRVRVLRVVAVHDVGRVLNPKGLESQFYGGILQGIGLTLMEDRVVDERTGLVLN
                     ANLQDYKVPTMPDSPEMIVRAVDIPDTAANHIGSKGAGEPPIIPTPAAIANAIYDAVG
                     VRVRHLPVTPRRLLETLEPGRTPQLEESGS"
BASE COUNT          752 a         1399 c         1298 g          759 t
ORIGIN      
        1 gccgcctggt ttgccagcac cgacatcacg cgcaggtcgt catgggtgaa gtcgccttgc
       61 ttcttgttta cggcggtcac caccccaatc gcatgcccgc gcgacaccat cggggccgcc
      121 agcacggagt gcgcccccat ctcctgccgc agggtatggt tcacccgctc atcagaggcg
      181 gcgttgttgc taatctgcac ctgcccggtg cgaaaagcca cgcccgatag cgactggtct
      241 accggcaggt ggtttgcgct cgcaagcttc ggggagttcc atccaatcgc cgcttccagc
      301 tcgtcgggcc tgctttcgct cggcaagaac accaggcacg actcggtgct caacatctcg
      361 ctgactgagg aggctatcgt ctgaaggatc tcctggagtc cggcggtacc cgccaggtcg
      421 gcggtcacgc gctgcatggt gacgctctcc accaggcggc gctcaagctc agcgttggcg
      481 gcggccagct cacccgtgcg ctcggtgact ttggcttcca gctccgagta ggaggcttga
      541 agctgccgcg ccatagagtt gaactgtcgg gccaactcct cgatctccac ggggccgctc
      601 acctgcaacc tgtgccgcaa tctccccgcg ctcaacgctt gcgaagccgc caccagcttt
      661 gctaccggcc tcgtcagcgt gcccgcgctc cgcgccacca gcagcacggc cagcagcatc
      721 gtgacgaggg cgagcaccaa cagccccgac agcaacgagt tcagcggagc gacgatgtcc
      781 gcccagcgct cctgggtgac cacgtaccag cgcagccagg tgaggtcgag gtcggcacgg
      841 gggaagagca gcgcgaggtt cagtggggcg tacccgatga tgtgctgctc gccgttgagg
      901 ggcgtaataa ttgcgctggg ttcgctggtg gtcaggtcgt tcagcgccag gctgggcaac
      961 atggtgccga cgagtttttg gtccgggtgc cagagcacgc gcccgtcgga cgcgacaaga
     1021 taagagatgc tgtgccctgc tccgccgttg gcggtggcgg ccctctgaag gccgaacggc
     1081 ctgcccagct tggggctgcc atagaaaatg acaccttcta ccatgccccc gtaaccgccc
     1141 ctgttgtcga tgaagggcag tgtgacgagg ctggccggtc tgccgtcgcg tagctcggtg
     1201 ggtatgataa cagtggctgg gccgatgcct ttgctgaggc tttcgtaagc ccgcttattg
     1261 agcacgtccc ccgctatggc cgcaaggttg gaggctatca gcttgccgtc aggcccggta
     1321 acctgcacca tatcgaactg gcggataaca gattccttct tcagttcgta gaagatgccc
     1381 gtgtcgtctt tggggtccaa aagcacaggg gagcttgcta cctgctggag gacctgcacc
     1441 gcaagggaca ggtggtcccc cacgcgttcg gcctgaatcc gcgcaagctc ggcgtcccgg
     1501 ctcttcgcga gcgactcgct gacctgcgtg tagcccacct gcccaacgac cgcgaaggcc
     1561 accacaagca gggtaagcgg tagcagggtc cgcaatataa gctggccccg caaggtgcgg
     1621 atgaaagaac gccccagggt ggcaggtttg cggtccgagt cggccgtatt cggttgctcg
     1681 acgggggtgc tttgcattct agacgggcca cgagttcagc ggaatggcaa tcaatactac
     1741 cagcagactc gcggtgaaga aaatagcgcc ctgccgcccc ttgtccgtgg aggtggcgtg
     1801 acggcccagg tccgcacgcg ccctgcccac gtgcgcgagc gcgacggccc ctaacattgc
     1861 cagcgggtgc agccagccta gccctacgtc accctgccag cgctggccca gcacccacac
     1921 gagcaagccg atgagaaact gtacgtccat agcaattgtg aaaaagaggc tggaccggtc
     1981 gccaactacg ccccagcggc gagtgccggt ggcggacacc aaggccaccg cgatggccag
     2041 cactgccgtt agcagcacga cccacctgaa aacactgtgt atggttacta gcaagtcgag
     2101 catggcattc tccagtaaac aaaagcgtct gtcgccttac gcggggcaga cagactatcc
     2161 ctgcatacca tattgtaccg aggttccagg gtgccctgca tcagcctttc agtgtactgc
     2221 ggccgccaaa tgccaccggc gctcgtgcat aaacttggag gctcaaggac ctggggcggg
     2281 agcagactcc gaccgcgacc cgcggcgcta ttctattact tgcgaaagcg cctgcttgaa
     2341 cagcccacgc agcaagggca ccttatagcc gttctgcgac agcggagtgg cggtagcgac
     2401 caggctatcg gccaggctgt cggcgtccag gcgatcagtt tcctgtccta ccagttgctc
     2461 ttcgatttct gggaaacgca ggggtatggg cgcgactccc cccagagtca accgcgcttc
     2521 ctcgatgctg cccgccgcca cgcgcacata caggccgacc gaaaccagtg caaacgccca
     2581 actggcgcgg ggcatcgcct tgaggtagac agagcgtccc gacgcgaaag ggctgagcac
     2641 aatctccgta atgaccgctc ccgggggcat ggcaaagctg ttgcgccgct cgggtgtggg
     2701 taccacgtac aggtcgcgca gtggtgcctc ggtttcaccc tggggtgttt gcatcctcac
     2761 taccgcgtcg aaaagcaaca aggcgaccgc cgggtcggag gggtgaacgc tcacgcagcg
     2821 gctctcgcca ggcgagttca ggaagatagc gtgttgctcg ttctcgccgt tcctcgcgaa
     2881 gcacacttct ccacccttga gccagcagtt gtgctggccc ctgtaatacc agcacctcgg
     2941 ctgttggagg agattccccc caatcgtgct catgttgcgc aactgggggg tagcggcaag
     3001 cccgcacgcc tgcgccagcg ccgggtagcg ctcgcctacg agcgggtgcg ccgctatgtc
     3061 gctcagcgtg gacatggcgc ccaggtgcaa cgtgccgcca cggtcctcaa catagctaag
     3121 gcccgtgagg ccgcctatat caaccagggc acgcggcacg actatctcgt ccttcatcag
     3181 cgggagcagg tccgtcccac ccgccagcag cgccgccccg tcgccctgcc caacggcgga
     3241 gagtgcggct tcaagcgtgc ctgccttcgt gtagtcgaag tttcgcatta cgaaccactt
     3301 tcttccagct ggggcgtccg ccccggctcg agcgtttcca gcaaccgcct gggcgtgacc
     3361 ggcaggtggc gcacccgcac tcccactgcg tcgtatatgg cgttcgcgat ggcagcggga
     3421 gtggggatta tgggcggctc gcccgcgccc ttcgagccga tgtggttggc cgccgtgtcc
     3481 ggtatgtcaa ccgcccgcac gatcatctca ggagagtcag gcatggtcgg tactttgtag
     3541 tcctgcaaat tggcgttcag aacaaggccc gtgcgctcgt ccaccacgcg gtcttccatg
     3601 agcgtcagac cgataccctg caagatgccg ccgtagaact ggctctcaag ccccttcggg
     3661 ttgagcactc tcccaacgtc gtgcaccgcg accacgcgca acacgcgtac ccggccggtt
     3721 gcaaggtcca ccgccacatc ggcaatctga gcgccccagg tgcgcagcgg ctcggccggg
     3781 ttcggccccc tgaagccctt gcccacgatc atgtagtccc ctacctcgga gagcaggtcg
     3841 cctatgctgc gcttgccctc ttcgcgaccc tctaccgtga tgtggccgtc cctgacctgt
     3901 agctggctgg cgggcacctc gaagaagtac gaagcgatct ccagtagctg ttgctttgcg
     3961 tcttcggcag cgccgcggac cgccggccca accgacggca cagtgatgct tccccagctt
     4021 gcgggtgcga acggcccctt ctgcgtgtcg ccaagccgga agcgcaccac atcgaggggc
     4081 acgcccagct cttcggccgc gacctggctt agcgccgtcc tgatgccagt accgatgtcc
     4141 tgggtgccgc aaatcacttc gaccgtgccg tctgagttga ggcgcaccag cgcgtgtgcg
     4201 ggagggcc
//
LOCUS       DASRWZ010000291         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_229405, whole genome shotgun sequence.
ACCESSION   DASRWZ010000291 DASRWZ010000000
VERSION     DASRWZ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229405"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VFH60_04515"
     CDS             complement(<1..1322)
                     /locus_tag="VFH60_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313075.1"
                     /translation="MLLSIALRSASVPAEAAVYTVANAPTNFAFPEFGAVWQRADGPV
                     AAGQVARSWLWGPEPGSTVEEPFAEADGGKRTVQYYDKARMELNEAEQDVNSAWRVTT
                     GLLVNEMVTGRVQVGAARFESRAPAAIFVAGDANAPTNPTYADFAPAIASKAPDRTGS
                     VIDDTMPRWDPALVREPHTYARYISETGHNIADVFWSYLNQQGQVSTKDGTLRTEQLF
                     DWVYLMGYPISEPYWAKITVDGKSYAALVQLFQRRVLTYIAEMPAGWQVQMGNVGQHY
                     HEWRYNRVGGTAGEQAKPGGTSVTPTPVLPEGAFVTVEGNQFVYAGAPIKLKGTNYWL
                     SDAPFSGTWASWNGPKVYEELKRAQALGINVVRVGIPFDHGDTMDVLWDSDKEMRKIS
                     MWIQRQMTQVLQIASTLGMKVIFTLFEWYDEQPSQGSREERPNIAYLEG"
     gene            1770..2126
                     /locus_tag="VFH60_04520"
     CDS             1770..2126
                     /locus_tag="VFH60_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313076.1"
                     /translation="MPGSSFPPPLPPPPPPSSQQQVGYPPSEAPRTAQGHSVLAALRV
                     GSPAKWQFWVGIALLAFVLTCCPCTLAVLIYGSDALADPGGFISSILGVGACLFLVAF
                     VLGIILLRVGRPRRLQ"
     gene            2243..>2866
                     /locus_tag="VFH60_04525"
     CDS             2243..>2866
                     /locus_tag="VFH60_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313077.1"
                     /translation="MLAAPSLLLVRWQLLPPAPFVLDLGDSSVSASHFYGVEAAPDRK
                     FRWSREMSAVSLPALASSVTISITLDPARAAGQPLPVVRLRVGDRQVGELASQAGWQT
                     YATNTGPGLSPDVRLVVESGTFYPGVADRRLLGVAVSQIAVASAPNRFGLIFPPLLLL
                     SLAALTPALGLFLAAILRIATEKRYTWLLLLALALIPVVFSLALTQDY"
BASE COUNT          479 a          924 c          880 g          583 t
ORIGIN      
        1 ccctccaggt aggcgatgtt aggccgttcc tccctcgaac cctggcttgg ctgctcgtcg
       61 taccactcga acagcgtgaa aatgaccttc atacccagcg tggaggcaat ctggagcacc
      121 tgggtcatct ggcgctgtat ccacatgctg atcttgcgca tctccttgtc gctgtcccac
      181 aacacgtcca tcgtatcgcc gtgatcgaac gggataccca ctctcacgac gttgatgccg
      241 agggcctggg cgcgcttcag ctcttcgtac actttggggc cgttccagga cgcccaggtg
      301 cccgaaaacg gggcgtcgct caaccagtag ttcgtgccct tcagcttgat gggcgcgccg
      361 gcgtaaacga attggttgcc ctccacagtg acaaaggccc cctccggcag cacgggtgtg
      421 ggggtgacgc tcgtgccgcc cggctttgcc tgctcgcccg ccgtgccgcc gacgcggttg
      481 tagcgccact catggtagtg ctggccgacg ttgcccatct gcacctgcca gccagcgggc
      541 atttccgcaa tgtaggtcag cacccggcgc tggaagagct gcaccagggc tgcgtagctc
      601 ttcccatcga cggtaatctt ggcccaatac ggctccgaga tggggtaccc cataaggtac
      661 acccagtcga aaagctgctc agtgcgcagg gtgccatcct tagtactgac ttgcccctgc
      721 tggttgaggt agctccagaa aacgtctgcg atgttatgcc cggtctcaga gatgtacctc
      781 gcgtaggtgt ggggttcgcg aacgagcgcg gggtcccacc ggggcatcgt gtcgtcaatt
      841 accgagccgg tgcggtcggg ggctttggag gcgatggcgg gtgcgaagtc cgcgtaggtg
      901 gggttagtgg gcgcgttcgc atctcccgcc acgaaaatgg ctgccggggc gcgcgactcg
      961 aacctggccg cgcctacctg gacgcgtccc gtcaccattt cgttcacgag caggccggtg
     1021 gtcacccgcc acgccgaatt cacgtcctgc tcggcctcgt tcaactccat gcgcgccttg
     1081 tcgtagtact ggacggtgcg cttgccgccg tccgcctcag cgaacggttc ttccacggtg
     1141 ctgccgggtt ccggccccca cagccacgaa cgggccacct gcccagcggc tacaggtcca
     1201 tcggcgcgct gccagactgc cccaaattcg gggaaggcaa agttggtggg cgcatttgcg
     1261 acagtgtaga ccgccgcctc tgccgggacg gaagcggaac gaagagcgat tgacagcagc
     1321 attattgctg cgacgaggag ggtcacacgc tgcattcgac gtgataaggc agaccgggac
     1381 atgaggttcc tttccatgtt tacacgccag caaagtaggt cgtagaatgg cgtggcccct
     1441 aagtatacca agtgtgaggc actatttagg gctcgctggg caaaactgag gcgaaagtac
     1501 tacatgagag agtattgacg gcggccacag acagagaggc agttggccgc cgcggagagg
     1561 caggggccgt accctgagta gtggcggtgt gcctgtgata taatgtggcg aaatccagcc
     1621 gaccagggga acgaccacgc ttgcagtgtc cacgttgtaa cacagctaac agtccagact
     1681 cgcgcttttg ccgcatgtgt ggtttacccc tgacacgaca gggcggcaca ggcggcgttc
     1741 cggtagagca gccacggccc caggctgggt tgccggggag ttcgtttccg ccacccctgc
     1801 caccgcctcc acccccgtcg tcgcagcagc aggtcgggta cccacccagc gaagcgccac
     1861 ggacggccca ggggcacagc gtgctggcgg cgctacgcgt gggcagcccg gcgaagtggc
     1921 agttctgggt aggcatcgca ttgcttgcct tcgtgctcac ctgttgcccc tgcaccctgg
     1981 cggtcctcat ctatggctct gatgccctgg ccgaccccgg cggatttatc agctctatcc
     2041 tcggagtagg ggcctgcctg ttcctggttg ctttcgtgct cggcatcata ctcttgcgcg
     2101 ttggccgccc caggcgcttg cagtagccgt aaacgtggac acgcataccc ccagcgagcc
     2161 atcaaaagca gaggggcagg acagcctccg aacctctgcg tggggtctca caaagtttca
     2221 actggcactc tgtgtcttct ttatgctggc cgcgccctcg ctccttctcg tgcgctggca
     2281 gcttcttcct cccgcgccct tcgtactcga cctgggcgac tcctcggtgt ccgccagcca
     2341 cttctatggc gtggaggcgg cccccgaccg caagttccgc tggagccgcg agatgtcggc
     2401 agtgtcgctc cccgccctcg catccagcgt aaccataagt ataaccctgg acccggcccg
     2461 tgcggcagga cagcccctgc ctgtcgtgcg cctgcgcgtg ggtgacagac aggtcggtga
     2521 gctcgcatcg caagcgggct ggcagaccta cgctaccaat acggggcctg ggttgtcgcc
     2581 agacgtgcgc ctcgtggtcg aaagtggcac tttctatcct ggcgtggccg acaggcggtt
     2641 gctaggggtc gcagtttcac agattgccgt agcttctgcg cccaaccggt tcggcctgat
     2701 ttttccgccg ctgctgttgc tttcgcttgc cgctcttacc cccgccctgg gcctgtttct
     2761 ggcagccata ttgcgtatcg cgaccgagaa gcgttacacc tggctcctgt tgcttgccct
     2821 ggccttaatt ccggtggtat tcagccttgc cctgacgcag gattac
//
LOCUS       DASRWZ010000292         4496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_983456, whole genome shotgun sequence.
ACCESSION   DASRWZ010000292 DASRWZ010000000
VERSION     DASRWZ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983456"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFH60_04530"
     CDS             complement(<1..397)
                     /locus_tag="VFH60_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6313078.1"
                     /translation="MLIVDVNACPQFVAGDETLLREVLHPHRDAAPVPYSLAHAVLAP
                     GTRSLSHRLRGSEVYYFLQGHGQMHIDGEREEVRAGHVVYVPPGATQFVVNTGEEQLA
                     FLCIVHPAWRADDEEVEEVEEVEEVEEVEE"
     gene            791..1093
                     /locus_tag="VFH60_04535"
     CDS             791..1093
                     /locus_tag="VFH60_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313079.1"
                     /translation="MAKQGQHNNDSNDSDQSKGPNNPSKSVAIDTGTPKKKETYAQQA
                     AEGKATDRQPQPQRNDWNEDTRDKPTIEDSPRARDSDIGSGRSGSDSNAGPGTRGG"
     gene            1095..1517
                     /locus_tag="VFH60_04540"
     CDS             1095..1517
                     /locus_tag="VFH60_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015194541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313080.1"
                     /translation="MGSTGGGGGGSARFSEEDRLWDQDLRPDDNAGQNEGIQGLHPEK
                     ADGVRTAYDIKDLHNRLEGFEDDELKQITVLPVGSRLEQNATYIDLRTHDREEFTARG
                     DMEAGPENWYVPKTEVDYQLWNRLIGVRNPERTGEADD"
     gene            complement(1573..2634)
                     /locus_tag="VFH60_04545"
     CDS             complement(1573..2634)
                     /locus_tag="VFH60_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015210504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET6313081.1"
                     /translation="MHIYEDRQEEDGLVIQSVVGQEVPIVPLRPAENHATSLTLRHQP
                     SADQHPAAVYLARLARGSRRTMSEALNTVAGILTSDRYDMQTLDWGGLRYQHTAAVRS
                     ALADLYAPATANKMLAALRGVLREAWRLGQMSAEDYHRAADLPAVRGTTPPRGRALDE
                     GELGQLFARCALDKTPAGLRDAAMLGVLYGCGLRRSELVGLDVADYVAKDGALHIRSG
                     KGNKARTNYATGGARTALEAWLKARGKQAGPLFCPINKAGKIATRRLTDQAVRKVLLK
                     RAREAGVERFSPHDLRRTMIGDLLDAGADISTVQRLAGHANVTTTARYDRRGEAAKRK
                     AAELLHVPVAPPTDEADAE"
     gene            2982..4445
                     /locus_tag="VFH60_04550"
     CDS             2982..4445
                     /locus_tag="VFH60_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HET6313082.1"
                     /translation="MEATERKSGGTTAKDLSVIASGQSQGIDIDPEAKTPPVVLSVTP
                     RFGSIFGGTSVQVKGRNFKPRSRVLFGLREARIVIYINAQTLICVTPPAQPGLVDVVV
                     VNPSGLRGVLPRGFLYSLVGQVVQNPENAAEQLEAGSGAIDLADLEAAAEMGAASSTM
                     ELEEGSDQIVEVDGLAELDDIDVQVPPDQRSRPIVSSYYPKQGPSIGGPGVTFVGAQF
                     RPRTRVFFAGRQAATIFINESTLVAITPGAPGPGSADIVLRNPSGLQSVIPNGFRYTR
                     GFLLEGLTGGARDQAAKGATKNAEPEAEELAEGIDLQQQPPVVTSVTPNRVDTNGGKT
                     VTIRGSNFRSGSTVTFGMLPATNVVFVSRSELRCTVPPRSTAGNVKVRVTNQGSMLTG
                     TLSMGFTYVQAPAPLLFGISPDTVSSFGNTGINVFGANFAPGCLILLGAGKGVANYID
                     STFVGFAAPPASPGTIFQVGVQNPDGQIACCAILRYV"
BASE COUNT          906 a         1394 c         1433 g          763 t
ORIGIN      
        1 cttcctctac ttcctctact tcctctactt cctctacttc ctctacttcc tcgtcgtccg
       61 ctcgccaggc cgggtgcacg atgcagagga acgccaactg ctcctcccct gtgttgacaa
      121 cgaactgggt cgctcccggc ggaacgtaga ccacatggcc tgcgcgcacc tcctcgcgct
      181 caccgtcgat atgcatctgg ccgtgccctt gcaggaaata gtatacctcc gagcctcgca
      241 gccggtgcga caaagaccgc gtgccgggag ccagtacggc gtgcgccagg ctgtagggga
      301 ccggagccgc gtcgcggtgg gggtgcagca cctcccggag caaggtttca tcgcccgcta
      361 cgaactgcgg gcaggcgttc acgtcaacta taagcatcta tcagggcctt tcgagtagta
      421 tcctgcttcc acggcgggag tgccgcgacc cttcatttta catcgctacg tgccagttcg
      481 catcaaaggg ggtaacacgc ccctcgtatc gaatggcaca ccccttgctt gcacgttttg
      541 gagcgtaagc tataccgccg catgctagca acgaaaggag cgccacgatg gtaaagaagg
      601 gacaggaaca ggtgagtcgt atggattcgc cgcgccaggg cgaagagcgc gaccccaagg
      661 aaggcaagat caagcacaag ggtggctcgg agagccagcg ccgcgcaact ccgcccagcg
      721 aaggtagctc caagggctaa ggagccggag aagcgagcgc aggcagtaat taccggaggt
      781 acttcaacat atggcaaagc agggacagca caacaacgat tccaatgaca gcgaccagtc
      841 gaaaggtccc aacaatccct ccaagagcgt agccatcgat accggcacgc ccaagaagaa
      901 ggagacctac gcccagcagg ccgctgaggg caaggccacc gacaggcagc cccagcccca
      961 gcgcaacgac tggaacgagg acacccgcga caagccgacg attgaggaca gcccgcgtgc
     1021 ccgcgactcc gatataggca gcgggcgcag cggcagcgac tcaaatgccg ggcctggcac
     1081 gaggggaggc tgatatgggc tcgactggtg gtgggggagg gggttcggcc cgcttcagcg
     1141 aagaggatag gctgtgggac caggacctgc gccccgacga caatgcaggg cagaacgagg
     1201 gtatccaggg cttgcacccg gagaaggccg acggtgtgag gaccgcctac gatatcaagg
     1261 acctgcacaa ccggctggag ggcttcgagg acgacgaact caagcagatt acggtcttgc
     1321 cggtgggcag caggctggag cagaatgcta cctatatcga cctgcgaacg cacgaccgcg
     1381 aagagtttac ggccaggggc gatatggaag caggccccga aaactggtat gtgcccaaga
     1441 cggaggtgga ttaccagctc tggaaccggc tcatcggcgt gcgcaacccg gaacgcaccg
     1501 gggaggccga cgattaggaa ccggctagtt cagacaagca aatctgcgga ataggcccct
     1561 atgaaggtgg gtctattccg cgtctgcttc gtctgtagga ggcgcgacag gcacgtggag
     1621 caactcggcg gctttgcgtt tggccgcctc gcctcgcctg tcgtagcggg ccgtggtagt
     1681 gacgttggcg tggcccgcca gccgctgcac ggtcgagatg tcggcccccg cgtcgagcag
     1741 gtcgccaatc atggtgcggc ggaggtcatg gggcgaaaag cgctccaccc cagcctccct
     1801 ggcgcgcttg agcagaacct tccgcaccgc ctggtcggta agcctgcggg tggctatctt
     1861 cccggcttta tttatggggc agaagagcgg cccggcctgc ttgccgcgcg ccttgagcca
     1921 ggcttccagg gcggtgcgcg cgcccccggt ggcgtagttg gtgcgggctt tgttgccctt
     1981 gcccgacctg atatgcagcg ccccgtcttt ggccacgtag tcggccacat caagccccac
     2041 aagctcgcta cgccgcagac cgcagccgta cagcactccc agcatggcgg cgtctctcag
     2101 gcccgcaggc gtcttgtcga gggcacagcg ggcgaatagc tggcccagct ccccctcgtc
     2161 gagcgcgcgg cccctgggag gcgtggtgcc ccgcacggcg ggcaggtcgg cggcacggtg
     2221 gtagtcctcg gcgctcatct gccccagccg ccaagcctcg cgcaacaccc cccgcagggc
     2281 ggccagcatc ttgttcgcgg tggcgggcgc gtacaggtcg gccagggccg agcgcaccgc
     2341 tgccgtgtgc tggtagcgca ggccgcccca atcgagggtt tgcatatcat agcggtccga
     2401 ggtaaggata cctgccaccg tattcaacgc ttcggacatg gtgcgccgcg agccacgggc
     2461 gagtctcgcc aggtagacgg ccgcggggtg ctggtcagcg ctgggctggt ggcgcagggt
     2521 gaggctggta gcgtgattct ccgcaggccg aagcgggact attggtactt cttgccccac
     2581 cacgctctgt attaccaggc cgtcctcttc ctgccggtcc tcatagatgt gcatgctctg
     2641 ttcttccctc taccttctac ccttacgaca cgttcaaccc ccgaaacgtt cctgaatgat
     2701 cgcagccata gattagcaca gacgctctat ggctgcgtcg ttattcgccg tgacgtgccc
     2761 gttccgccgg cggctgatgt tagccattgg tgaacattaa gtgagcaatg aatgacctgg
     2821 atgattagca gagagtggat gcactatgtt gcaggtccga caaacatgtt cagggccgca
     2881 gctgcagcta aagttcctgt gtggacgtgt aaacactggc atatcggaac atcgcaacat
     2941 cgggacgatt gaaacatgaa taacgaaaag gagaaaaggt gatggaagca acagaacgca
     3001 agagcggggg cacgaccgcc aaagacctct cggtaatcgc ctcgggccag agccagggca
     3061 tcgatatcga cccggaagct aagacacccc cggtggtgct gtcggtgact ccccgtttcg
     3121 gctcgatctt cgggggcact tcagtccagg tgaaaggtag aaacttcaag ccccgctcca
     3181 gggtgctatt cgggctgcga gaggcacgca tcgttatcta catcaacgcg cagactctca
     3241 tctgcgtcac gcccccggcc cagcctggcc tggtggacgt tgtcgtggtc aacccgagcg
     3301 ggctgcgcgg agtgctcccc aggggctttc tgtactcgct ggtggggcag gtggtgcaga
     3361 accctgagaa cgccgccgag cagcttgaag ccggcagtgg ggccatcgac cttgcagacc
     3421 tggaggcggc cgccgagatg ggcgccgcca gtagcacgat ggaattggaa gaaggcagcg
     3481 accagatcgt agaggtagat ggattggccg agcttgacga cattgatgtg caggtgccgc
     3541 cggaccagag gagcaggccc atcgtgagca gctattatcc gaagcaaggg ccgtccatag
     3601 gcggaccggg cgtcaccttc gtaggcgcgc agttccgccc gcgcacccgc gtgttcttcg
     3661 ccgggcggca ggcagccacc atcttcatca acgagagcac gctggtcgct atcacccccg
     3721 gcgcacccgg accaggctcc gccgacattg tgctcaggaa ccccagcggc ctgcaatcgg
     3781 tgatacccaa cggcttccgg tacacgcgcg gcttcctgtt ggagggtctg accgggggcg
     3841 ctagggacca ggcagccaaa ggtgccacga agaacgcgga gcctgaagca gaggagctgg
     3901 cagaaggtat cgacctacag cagcagccac cggtggtcac ctcggtcacg cctaataggg
     3961 tcgatacgaa tggcggaaaa acggtcacca tcaggggcag caatttccgc tcaggctcga
     4021 cggtgacttt cggcatgctg cccgccacca acgtcgtgtt cgtgagcagg agtgagctga
     4081 ggtgcacggt acccccgcgc tcgaccgccg gcaacgtcaa ggtgcgcgtc accaaccagg
     4141 ggagcatgct gacgggcacg ctgtcgatgg gcttcaccta cgtccaggca ccggccccgc
     4201 tcctgttcgg catcagcccg gatacggtca gctcgttcgg aaacaccggg atcaatgtgt
     4261 ttggggccaa cttcgcccct ggctgtctga tcctcctggg cgcgggcaag ggcgtggcga
     4321 actacataga cagcaccttc gttggtttcg ccgcgcctcc agcatcaccc ggcacaatct
     4381 tccaggtagg agtgcagaac ccggacggcc agatcgcgtg ctgtgcgatc ctcaggtacg
     4441 tctgaggcga caaggcgagc atcgcggttg aactcgtgta gtagcataca aagcta
//
LOCUS       DASRWZ010000293         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_641259, whole genome shotgun sequence.
ACCESSION   DASRWZ010000293 DASRWZ010000000
VERSION     DASRWZ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641259"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFH60_04555"
     CDS             <1..192
                     /locus_tag="VFH60_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313083.1"
                     /translation="PYAESASAEFEVRPGTHNYVVPLGADWSSMETVEGLRLDPVESG
                     NPSEGDLNKVCVEEIRLLP"
     gene            complement(214..978)
                     /gene="rlmB"
                     /locus_tag="VFH60_04560"
     CDS             complement(214..978)
                     /gene="rlmB"
                     /locus_tag="VFH60_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET6313084.1"
                     /translation="MAEWLYGRHAVEETLKAGRRKVFRVLLSEGGKGGRSPADDPLAR
                     IQAEARRQGASLERVPPARLDALTRVGQHHQGVAAEVSGYPYRTLAEVVALCREAGPN
                     ALVLLLDSIQDPQNFGTLLRSAEAVGATAVVMLERRQVEVTAAVVNASAGAVEHLTVC
                     QTNNLPRGIEALQEAGLWVYALQAESDAVPYTMADLKGPLGLVVGSEGSGVGRLVRER
                     CDGALEIPMRGKIESLNAAVAGSIVLYEALRQRTTV"
     gene            complement(1048..1395)
                     /locus_tag="VFH60_04565"
     CDS             complement(1048..1395)
                     /locus_tag="VFH60_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET6313085.1"
                     /translation="MPVYEYQCKNCGERFEKIQPVMSDPLTECLLCGQGPIRRVLHPV
                     GVIFKGSGWYVTDNRGSGRSGNSSGSKGDEDGKSSSSAEASTDKPKADASNNTSGSTS
                     GGTSSTTSKSSDE"
     gene            complement(1478..3145)
                     /locus_tag="VFH60_04570"
     CDS             complement(1478..3145)
                     /locus_tag="VFH60_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313086.1"
                     /translation="MPHSRDTDSETPRAPKIGKVIDLQGYLAAERALDRFLATAPSHP
                     QYQARLSEVAGYGPSLLAVIIRRLDAYRPDEIEELGRAATAFPDRDLAVAHLHKAAAD
                     RRNSDSRRLGAMLVLEYCLGEPPDEDFVGTLHDPLAALAYSLRGALRGAFKSQILRSY
                     VHTLLAQSSELLFGIFSRLTSIERDTPTEVARLLALHPDLELSMSVVEALAAQGSRGS
                     VQTLALLEPSLAPEVSHAANRAMQKLRLSGYSPRVLRPPVEGCRALISPIDGSGNRLL
                     MLIAPESESGDGSTVLELFVNEAVGVVEASGTPDAMQADLPMVARPGFVHRHSLRTWM
                     GMPLGEDTRGGSYDGSRTAGHVPLLEADFLYGLEVLREAVQNNWATSGPLPLDYCLLS
                     HICWSYSGGLDSADLRAAWDLDSDSPLTAREADLLTNSVFDSWYLSGESAMQVAHDIS
                     TLSGGPPRELTDDSWRFLLPALIRLAHDEFGPDVRQRYSDRLRRMSEWLHMSGEDHDA
                     ECARSAAQTILKAPPETNLLVLRLVQRGILVALNRLAADSRHHNQTH"
BASE COUNT          591 a         1101 c         1017 g          604 t
ORIGIN      
        1 ccatatgctg aaagtgcctc ggcagaattc gaggtccggc ccggcacgca caactacgtc
       61 gtgcctctcg gcgcggactg gagcagcatg gagacagtag agggactgcg acttgacccg
      121 gtggaaagcg gcaatccctc cgaaggggac ttgaacaagg tctgtgtcga ggaaatacgg
      181 ctgctaccct gaccgcagca cgagccacac tgtctagacg gtggtgcgct ggcggagcgc
      241 ctcgtagagc actatcgagc ctgccacggc ggcgttgagc gactcaatct tgccccgcat
      301 cggtatctcc agcgccccat cgcacctctc gcgcaccagc ctgccgacgc cgctgccctc
      361 cgaaccgacc accagcccca gcggcccttt caggtcggcc atcgtgtacg gcacggcgtc
      421 actctccgct tgcagggcgt atacccacaa ccccgcttct tgcagggctt ctatgccgcg
      481 cggcaggttg ttggtctggc agactgtgag gtgctccacc gcgcccgccg aggcgttcac
      541 cacagccgcc gtcacctcca cctgacgccg ctcaagcatc accacggcgg tcgcgcccac
      601 cgcctccgcc gagcgcaaca aggtaccgaa gttctgcggg tcctggatgg agtcgagcag
      661 cagcacgagc gcattcggcc cggcttcgcg gcacaaagct accacctcgg cgagcgtgcg
      721 ataggggtag cccgaaacct cggcggctac tccctggtgg tgctggccga cgcgcgtaag
      781 ggcatctagc ctggctgggg gcacccgttc gagggatgcg ccctgccgcc gcgcctccgc
      841 ctggatgcgg gcaagggggt cgtctgccgg gcttctgcca cccttgcccc cttcgctcaa
      901 cagcactcga aagaccttgc ggcggcccgc cttcagcgtc tcctctaccg catgccttcc
      961 atacagccac tcggccatga accctcctct cccgcaaaac aacaggcgac cgggtggtcg
     1021 cctgggtgcc tcggccatgt tgagacgcta ctcgtccgat gacttcgagg tcgtgctgct
     1081 agtgccacca ctcgtgctgc cgctcgtgtt attgctggcg tcggccttcg gcttgtcggt
     1141 ggatgcctcg gcggaagacg acgatttgcc gtcctcatcg ccctttgagc cgcttgagtt
     1201 cccggacctg cctgagccgc ggttgtcggt gacgtaccag ccgctgccct taaaaatcac
     1261 ccccaccggg tgcaacacac gccgtatggg accctgcccg caaagcagac actccgtcag
     1321 gggatccgac atcacgggct gtatcttctc gaaccgctcc ccgcagttct tgcactggta
     1381 ttcataaact ggcatgttat ctcttctttg ccctctctaa gatctgctgt tacggcacag
     1441 ctatgaatat aagttgccgc acgtggcaac gaaaagtcta gtgtgtctgg ttgtggtgcc
     1501 tgctgtcggc ggcaagcctg ttcagcgcca ccagtatgcc gcgctgcacg agcctgagca
     1561 cgagcaaatt ggtctcaggt ggggccttta gtatggtctg cgccgccgac cgcgcgcact
     1621 ccgcgtcgtg gtcctcgccc gacatgtgca gccactcgga catgcgccgc agccggtccg
     1681 agtagcgctg ccgcacgtcg gggccgaact cgtcgtgggc cagccggatg agggcgggca
     1741 gcagaaaacg ccagctatcg tcagtcagtt cccgtggcgg gccgccgctg agtgtgctga
     1801 tatcgtgcgc cacctgcata gccgactccc ccgacaggta ccagctatcg aataccgagt
     1861 tggtcagcag gtccgcctct ctcgcggtca aggggctgtc gctatcgagg tcccaggccg
     1921 ccctcaggtc ggccgagtcg aggcccccgc tgtacgacca gcagatgtgg ctcagcaggc
     1981 aataatcgag cggcagcggg ccggaggtgg cccaattgtt ttgcaccgcc tcgcgaagta
     2041 cctccaggcc gtacaggaag tccgcttcca gcagtggcac gtgacccgcg gtccgcgagc
     2101 cgtcatacga gccgccccgg gtgtcctcgc ccaacggcat gcccatccag gtgcgcaagc
     2161 tatgcctgtg tacgaagccg ggccgcgcga ccataggcaa atcggcctgc atggcatccg
     2221 gcgtaccgct cgcctccacc acgcccaccg cctcgttcac gaacagttca agcaccgtgg
     2281 acccgtcgcc gctctcggac tccggcgcga tcagcatgag gaggcgattc ccgctgccat
     2341 ctatggggct gattagggcg cggcagccct ccacgggagg ccgcaacacg cgaggactgt
     2401 atcccgaaag ccgcagcttc tgcatggcac ggttggcggc gtgggagacc tcaggggcca
     2461 ggctaggctc aagcagggcc agcgtctgca ccgagcctcg gctaccctgc gccgccaacg
     2521 cctccaccac ggacatggag agttccaggt cggggtgcaa cgccagcagc cgggctacct
     2581 cagtgggtgt gtcgcgctcg atggaggtga ggcgactgaa tatgccgaag agtagctccg
     2641 aggactgtgc cagcaaagta tgcacgtaag accgcagaat ctgcgacttg aatgcccctc
     2701 ggagcgcgcc ccgcaaggag taggcgaggg ctgccagcgg gtcgtgcagg gtgccgacga
     2761 agtcctcgtc gggcggctcg cccaggcagt attcaagcac cagcatagcc cccaggcggc
     2821 gcgagtcgga gttgcgcctg tcggccgccg ccttatgcaa atgggccacc gccaggtcgc
     2881 ggtcaggaaa cgcggtggcc gcgcgcccca gttcctctat ttcgtcgggg cggtaagcgt
     2941 caagcctgcg gatgatgacg gcgagcagcg aaggcccgta accggctact tcgctgagcc
     3001 tggcctggta ctgggggtgc gaaggcgcgg tagcgaggaa gcggtcgagc gcgcgttccg
     3061 ccgccaggta gccctgcaag tcaattactt tgcctatttt cggggcgcga ggagtctcgc
     3121 tgtccgtgtc gcggctgtgt ggcacgcata acctctctgg caaagcacta tggacgtgat
     3181 ttggaaattg gaatttcgga tttcgaattt ataggatacc tactaaattc gattctcgaa
     3241 atcccaattt ccaaattgaa aagcgtgatg cggtacttcc attataccgc atcacgcgtc
     3301 agttttactc aag
//
LOCUS       DASRWZ010000294         1566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_298988, whole genome shotgun sequence.
ACCESSION   DASRWZ010000294 DASRWZ010000000
VERSION     DASRWZ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298988"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="VFH60_04575"
     CDS             <1..1469
                     /locus_tag="VFH60_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6313087.1"
                     /translation="LPVVTTDPVLAHLRQAEEHYEQFRQQGERQAGELTEARLEVGEL
                     VASTGSLQSSLQAAQEQAEQHAARAQELQALLDRAQESVSRLEASLNETQELREQERR
                     AALRYRDRAAQMAEGFKQIHRAMFEGGVYGLILKACLTITGATRGLYVTTRGGIDNLR
                     IRAAEDIDGYPESPPSEFVKAICKRVLEENDSLVCNSEQELSDLPKPQREGEHFQNLV
                     ATPVVLLRSLNGIIIAADKLNGDFDEGDIETLLSVGDQAAIAVENKHLQQELQNSYVA
                     TVSMLADAVEAKDPYTHGHCDLVSRYARLIAEKMELEQQDRAVICYAALLHDVGKIGV
                     SDGVLNKPGPLLPEERELMRSHVRVGHDLLSKVPALSRVADVVLHHHEWYDGSGYPDG
                     LRGDQIPIAARIVCCVDAYCAMITKRSYKEAYSVDRAREELLRCAGTQFDPTVVQIFL
                     SILDTTGGELIDDDEEEDAACGVLPGFYNLRELTQPVQ"
BASE COUNT          330 a          492 c          517 g          227 t
ORIGIN      
        1 ctttacccgt ggtgactacc gacccggtgc tggcgcacct gcggcaggcc gaggagcact
       61 acgagcagtt ccgccaacag ggggagcgtc aggccggcga gcttaccgag gcgaggttgg
      121 aggtagggga gttggtggcg agcaccggca gcctccagtc aagcttgcag gctgcccagg
      181 agcaggccga gcagcacgcc gcgcgcgcgc aggagctcca ggccctgctg gaccgggcgc
      241 aggagtcggt ctcccggctg gaggcgagcc tgaacgagac gcaggagttg cgcgagcaag
      301 aacgcagggc cgccctccgc taccgcgaca gggccgctca gatggccgaa ggcttcaaac
      361 agatacaccg cgccatgttc gaggggggcg tgtacggcct catcctgaag gcgtgcctca
      421 ccattacggg ggccacgcga gggctgtacg tgactacgcg gggcgggatt gacaacctgc
      481 ggatacgcgc cgccgaggat atagacggct acccggagtc gcccccttcc gagttcgtca
      541 aggctatctg caagagggtt ctcgaagaga acgactcgct cgtgtgcaac agcgagcagg
      601 agctatccga cctgcccaag ccccagcgcg aaggcgagca cttccagaac ctcgtcgcca
      661 cgcccgtcgt gttgctccgg agcctcaatg gcatcatcat cgccgccgac aagctcaacg
      721 gcgacttcga tgagggcgac atagagacgc tgctgagcgt gggcgaccag gcggccatcg
      781 cggtcgaaaa caagcacctg caacaggagc tacagaactc gtacgtcgcc acggtgagca
      841 tgctagccga cgcggtggag gcgaaagacc cctacaccca cggccactgc gacctcgtat
      901 cgcgctacgc tcgcctcatc gcggagaaga tggagcttga gcagcaagac cgcgcggtca
      961 tatgctacgc cgccctgctg cacgacgtgg gcaagatagg cgtcagcgac ggcgtgctca
     1021 acaagccggg gccgctgctg cccgaagagc gcgagctgat gcgctcgcac gtgcgggtgg
     1081 gccacgacct gctgtcgaag gtgcccgcgc tgtccagggt ggccgacgtg gtgctgcacc
     1141 accacgagtg gtacgacggc agcggctacc ccgacgggct gcgaggcgac cagataccga
     1201 ttgccgcccg catcgtgtgc tgcgtggacg cctattgcgc catgattacc aagcgcagct
     1261 acaaggaagc ctacagcgtg gaccgcgccc gcgaagaact cctacgttgc gcgggcaccc
     1321 agttcgaccc cactgtggtg cagatattcc tctccatcct ggacaccacc ggcggcgagc
     1381 tgatagacga cgatgaggaa gaagacgccg cctgtggcgt gctgcccggc ttctacaacc
     1441 tccgcgaatt gacccagccc gtacagtgag cttctgggca ttaggaacga ttagtagacg
     1501 aaaacgcaaa gacgcaaagg acgcaaatga cgcgtaaaga gtagtaaggt ttagtgggaa
     1561 caatgg
//
LOCUS       DASRWZ010000295         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_847345, whole genome shotgun sequence.
ACCESSION   DASRWZ010000295 DASRWZ010000000
VERSION     DASRWZ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847345"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1761)
                     /locus_tag="VFH60_04580"
     CDS             complement(<1..1761)
                     /locus_tag="VFH60_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6313088.1"
                     /translation="MGKYEQDSAILPTSGNAETLDGVVDKITYVNEENGYTIGRLKVP
                     RQKQLVTIAGHLPAVNVGEGLRVEGHWIQHPQYGRQFQVSRYQTAAPGTVTALKKYLS
                     SGLLKGVGPVLAEHIVNTFGLDTVQVLDSTPHRLDEVPGLGSRKAQLIAEAWAERRTL
                     KDLMVFLQAQGVPIVLAARILRKYGTAAETVVREQPYRLPAEVYGVTFQVADSIASQA
                     GIPLNALQRVGAGVAQVLRDAASSGHVYLPLDDLLYGASQLLSLSTDTVRATLPELQQ
                     AGFLVLAQGQGDGEKNGQQRAYLTEMHTAEESVATALARISATGGDRLSAFQRVDWDS
                     AWRFLATLETVHLTERQQEAIRATLSQRVVVVTGGPGTGKTTTLRGIVRLLQAKGRTV
                     ALAAPTGRAAKRLTEATGVGASTIHRLLELKPGGSYQVRAPIEADLVVVDEASMMDLP
                     LADALLNAVPQGAHLLLVGDADQLPPVGPGSVFKDVILSGTVPVITLETIFRQPEGSA
                     IISNAHRINAGRTPRTGKGIMDFFFLPQPEPAACAQLVVDLATRRLPQHFKLDPLDDI
                     QVLAPIYGGVCGVDALNVNLQ"
     gene            complement(1771..2229)
                     /locus_tag="VFH60_04585"
     CDS             complement(1771..2229)
                     /locus_tag="VFH60_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET6313089.1"
                     /translation="MNKVQLIGRLGADPEMRYTGSGMPVTTFSLATNRQWTDREGNAQ
                     DETDWHNIVAWDRLAQICNEHLAKGRLVYIEGRLRTRTYEANGQKQYRTEIVASDMLM
                     LDSAAGAEHTNGRADAEKPAPAKVAAQASGRGKGRAAEPREVDDEDELPF"
     gene            complement(2314..>2841)
                     /locus_tag="VFH60_04590"
     CDS             complement(2314..>2841)
                     /locus_tag="VFH60_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313090.1"
                     /translation="GNIESRTVLLEWDEGEGGRFARFPYRLTISNGPGKKTTTGGVAP
                     AGEPTARLTMRIPELDMIKLMLALGSYVQAYETAHHHRLVANRLNELRDKLAERANHH
                     DVRPSAPERSPERQVERPAAARPLNEVRTGSSTARDGAGSGVARPALRPVSGGSKEPI
                     DLTPRTGRNGATRAG"
BASE COUNT          423 a          953 c          928 g          537 t
ORIGIN      
        1 ctgcaggttc acgttcagag cgtccacacc gcacacccca ccgtagatgg gggcgagcac
       61 ctgtatgtca tccagcgggt cgagcttgaa atgctgcggc aggcggcggg tagcgaggtc
      121 aaccacaagt tgcgcgcagg cggcgggttc cggctggggc aggaagaaaa agtccattat
      181 gcccttgccg gtgcggggcg tccgtccggc gttgatgcgg tgggcgttcg agatgatggc
      241 gctgccctcc ggctggcgga aaatcgtctc caacgtaatc accggcaccg tgccactgag
      301 gatgacatcc ttgaacaccg agccagggcc gaccgggggt agctggtccg cgtcccccac
      361 gagcagcagg tgcgccccct gcggcaccgc gttgagcaag gcatcggcca gcggcaggtc
      421 catcatggag gcctcgtcca ccaccacaag gtcggcctcg atgggcgcgc gcacctggta
      481 gctgccgccg ggcttaagtt ccagcaggcg gtgaatcgtg ctggcaccca cgcccgtggc
      541 ctcggtgagc cgcttggcgg ccctcccggt gggcgcggcc agggctaccg tgcggccctt
      601 cgcctggagc aggcgcacga tgcccctgag cgtcgtcgtc ttgcccgtgc ccggcccgcc
      661 cgtgaccacc accacgcgct gtgagagggt ggccctgatg gcctcctgct gccgttcggt
      721 caggtgtacc gtctccagcg tcgccaggaa ccgccacgcc gagtcccaat cgacgcgctg
      781 gaaagccgat agccggtccc ctcccgtcgc cgatatcctg gccagcgctg tcgctaccga
      841 ctcctcagcc gtgtgcatct ccgtcaggta cgcgcgctgt tgcccgttct tctcgccatc
      901 tccctgcccc tgcgcgagca ccaggaagcc cgcctgctgt agctcaggca gtgttgcgcg
      961 gacggtgtca gtagagagtg acagtaactg cgaggcccca tagagcaggt cgtccagagg
     1021 caggtatacg tggccggagg aggcggcatc gcgcagcacc tgggccactc cagcgcccac
     1081 gcgctgcaag gcgttcagcg ggatcccggc ctgggaagca atggagtccg ccacctggaa
     1141 ggtgactccg taaacctcag cgggcagccg gtaaggctgc tctcgcacga ccgtctcggc
     1201 ggcggtgccg tacttgcgca gtatgcgtgc ggcaaggacg atgggcacgc cctgcgcctg
     1261 gaggaacacc atcaggtctt tcagcgtgcg ccgctcggcc caggcttcag cgatcagttg
     1321 cgccttacgg ctgcccagcc ccggcacttc atccaggcgg tggggcgtgc tgtccagcac
     1381 ctgcacggtg tcgagtccaa aggtgtttac aatgtgctcc gcgagcaccg gccccactcc
     1441 cttgagcaga cccgagctga ggtacttctt gagggcggtc acggtgccgg gggcggccgt
     1501 ctggtagcgg ctcacctgga actgcctgcc atactgcggg tgctgtatcc agtgcccttc
     1561 gacgcgcagc ccctcgccca cattaacggc gggaaggtgc cccgcaatgg tcaccagttg
     1621 cttctggcgg ggcaccttca ggcggccaat cgtgtagccg ttctcttcgt ttacgtaggt
     1681 gatcttgtcc acgaccccgt ccagcgtctc ggcgttgccc gaggttggga ggatcgcgga
     1741 atcttgttcg tacttgccca tagtgcagag ctagaagggt agctcgtctt cgtcgtccac
     1801 ctctcgcggc tcggctgccc tgcccttgcc acgaccgctg gcctgggctg ccaccttcgc
     1861 gggcgcaggc ttttcggcgt cagcccggcc gttggtgtgc tcggcaccgg ccgccgaatc
     1921 gagcatcagc atatccgagg ccactatctc cgtgcggtac tgcttctggc cgttggcctc
     1981 gtaggtacgg gtccgcagcc gtccctcgat gtagacgagc cgccctttcg ccaggtgctc
     2041 gttgcaaatt tgagccaggc ggtcccaggc cacgatgttg tgccagtcgg tctcgtcttg
     2101 cgcgttgccc tcgcggtcgg tccactgccg gttcgtcgcc agggagaatg tagtcaccgg
     2161 catgccgcta ccagtgtaac gcatctcagg gtcggcaccc aagcggccga tcagctgtac
     2221 tttgttcaaa ctcctcgcca tctcgctatc tcctcttatc aactctaact actctgcgaa
     2281 tctattctct tgtgcgccga taggacgtta gacctacccc gccctcgtgg ctccatttcg
     2341 ccccgtccgg ggggtcaggt caataggctc cttgcttccc cccgacaccg ggcgcagagc
     2401 cggacgtgct accccagaac ctgcgccgtc ccgcgccgtg gacgagccgg tacgaacctc
     2461 atttaggggg cgtgccgccg cgggcctctc cacttgccgc tcgggcgagc gttcgggggc
     2521 ggaaggacgc acgtcgtggt ggttggcgcg ctcggccagc ttgtcgcgca gctcgttcag
     2581 gcggttggct acaagcctgt ggtggtgcgc ggtctcgtat gcctgcacgt aagaaccgag
     2641 ggcaagcatg agcttgatca tgtccagttc ggggatgcgc atggtgagcc gggcggttgg
     2701 ctcaccggcc ggggcgacgc cgccggtggt ggtcttcttg ccggggccgt tgctgatcgt
     2761 gaggcggtag gggaaacgtg cgaagcggcc gccctcgccc tcgtcccact cgaggaggac
     2821 ggtgcggctc tcgatgttgc c
//
LOCUS       DASRWZ010000296         4643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_778957, whole genome shotgun sequence.
ACCESSION   DASRWZ010000296 DASRWZ010000000
VERSION     DASRWZ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778957"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..867)
                     /locus_tag="VFH60_04595"
     CDS             complement(85..867)
                     /locus_tag="VFH60_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313091.1"
                     /translation="MEIAPTPGPRSLAPDPLFIVFGGGCFGSHHVRHIEKGRKRGRIS
                     PDARIVMVDRNARPPALDVPEVGDNPYLTLVQSDWLEYMKANWDALPPGTEVVPAPVA
                     PHLAFEWLVWSLRSRLGEEAIGVEPLEHQFGGLPYEYLAPSGARYISAADWICPTNCR
                     APHVCPITRDTRTWELERTVNGYADAHPEQFATAVVFQPRFRVPGVDVLLISEYIGAR
                     DRLLALAGKPEAMGKGVVVGTVSPCHGAVSLMRLGSPVTAIR"
     gene            complement(909..1862)
                     /locus_tag="VFH60_04600"
     CDS             complement(909..1862)
                     /locus_tag="VFH60_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HET6313092.1"
                     /translation="MKLSGLHHVSAVTGRAAENVRFYTQVLGLRLVKKTVNQDDVSMY
                     HLFYGDEAGHAGTEVTFFDWPHAGKDSPGAGSITAIALRVPGREALDWWEGRFTSLDV
                     PHEGIVERGGRASMAFTDPEGQRLILADDGGAPGGTPWAESPVPAEYGIRGLHAVTIV
                     VRRLEPTALVLTEAMGFRQVGEYPSPEDATQKVIVFAAGDGGPGTEVHVEVRPDARLG
                     QVGIGGVHHVAFRTPNEEEHKRWRQRLAEMGLRVTPVIDRFYFRSIYFREPGGVLFEI
                     ATDGPGFATDEDVKHLGERLALPPFLEPYRTEIEANLQPIR"
     gene            complement(2083..2463)
                     /locus_tag="VFH60_04605"
     CDS             complement(2083..2463)
                     /locus_tag="VFH60_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET6313093.1"
                     /translation="MHLESLDLHGYILADAILVVVRRYNRLLSSGAAGAEMYALEIVH
                     GKGRGDTGSVIRDTLREYLREHGKRISGWDAKLTMRGAHDALEKCGILGYIHGEDIDG
                     NSGKTVVIPRRPLGSPHPWLKYRA"
     gene            complement(2587..>4643)
                     /locus_tag="VFH60_04610"
     CDS             complement(2587..>4643)
                     /locus_tag="VFH60_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HET6313094.1"
                     /translation="QFQEKPSWGEVFSDTVNTGIYILEPKIFDYYEAGQVFDFSSQLF
                     PILLEKGDPMYGYVADGYWCDVGSIPDYMIASGDYLRGEVNLPKLAQETEPGSGIWVQ
                     EEVEIARDAHISGPVFLGTGVKIKSGATVRGPAVIRDFSVVDNRATVDRSIIWRNCYI
                     GERAEVRGAILQPQVNVKTQAMIFEGAVIGDGTIIGTGAVIHPNVKIWPSKEVESGAT
                     VTTSLIWGAQSRRNLFGRWGVTGLVNVDLTPEFAAKLGAAYGGMLAKGTTVTVNRDSH
                     YTPRMIKRALIAGLPSAGINVLDLRNVPIPVARHYTRTISAAGGVHVRLSPFDARVVD
                     IKFFDKRGLDLDKATERKVEGLFFREDFRRVYLDDVGRIDYAPDVEQRYGSDYLKNLN
                     CEAVQHGDERYSRIAIDYANSSSALVLPNLLAKLDADVVAINAAVQELSLYQTPEQFE
                     QAMDRLGAITQTLKAGFGVRLDTGGERIYFVDDQGQRVSGMATLAAMSELALAQHGSD
                     GMLVVPVYAASIFEDIAAKYGAEVIRTRFNSYALMTAALRSGVVMAGDGQGSFIFPQF
                     QPSADGLFAIAKLMEMTAQQGRSFTEVLRSLPHYAWASSRVPCRWEDKGRVMRILNEQ
                     YRDSGTKQVDGVKINLGHGEWALILPDADRPLFHVIAEADTDLAARQLVDKYAGLVQG
                     LQ"
BASE COUNT          847 a         1440 c         1403 g          953 t
ORIGIN      
        1 gacttcgtat atgtagcccg caactttagt cgtcgggcgg ggctggcgct gttgcatccg
       61 tggcgtcttc acgttttgct atctttaacg tatcgccgta acagggctgc cgagcctcat
      121 caggctaacc gccccatggc agggcgatac cgtgcctacc accacaccct tgcccatagc
      181 ttcgggtttc ccggcaagcg ccagcaggcg gtcgcgtgcc cctatatatt cgctgataag
      241 tagcacgtcc acgccgggca ccctgaagcg gggttggaag actacagccg tagcgaactg
      301 ttcagggtga gcgtctgcgt agccatttac ggtgcgctct agctcccagg tgcgggtgtc
      361 gcgggttatc gggcagacgt ggggagcgcg gcagtttgtg gggcagatcc agtctgccgc
      421 gctgatgtac cgcgccccgc ttggggccag gtactcataa ggcagcccgc cgaactggtg
      481 ctccaacggc tctactccaa tggcctcttc tcccaaccgc gagcgcaaac tccataccag
      541 ccactcgaac gccaggtgcg gggctacggg cgcaggcact acctccgtgc cgggtggcag
      601 ggcgtcccag ttggccttca tgtattccag ccaatcgctc tgcacaagcg tgaggtacgg
      661 gttgtcgcct acctccggca cgtccagcgc gggcgggcgc gcgttgcggt ctaccatgac
      721 gatgcgcgcg tcgggcgaga tgcggccacg cttgcgcccc ttctcgatgt ggcgtacatg
      781 gtgagagccg aagcacccgc cgccgaacac tatgaataag gggtcagggg ccagggatcg
      841 gggaccaggg gttggtgcaa tttccatatg gtatgttgtc ctatacagcc gtgtttgctc
      901 tgcataactt accttatcgg ctgtaggttc gcctcgatct cggtgcggta aggctcaagg
      961 aagggcggga gggccaatcg ctcgcccagg tgcttgacat cttcatccgt cgcgaagccc
     1021 ggcccatcgg tggcgatctc gaacagcacc cctcccggct ctctgaagta gatcgagcgg
     1081 aagtagaagc ggtctatcac aggcgttacg cgcaggccca tctcggccaa tcgttgccgc
     1141 caccgcttgt gttcctcctc attgggcgtg cggaacgcta cgtgatgcac gccccctatc
     1201 cccacctggc cgagtcgtgc atcggggcgc acctcgacgt gtacctcggt gccaggccct
     1261 ccatcgcccg ccgcgaacac gatcaccttc tgtgtggcat cttccggtga cgggtactcc
     1321 ccgacctgcc ggaagcccat cgcctcggta agcaccaggg ctgtcggctc caaccgcctg
     1381 accacgattg tgaccgcgtg cagcccgcga atgccatatt cagccgggac gggactctcg
     1441 gcccagggcg tacccccagg cgctccacca tcgtcggcca gtatcagcct ctgcccctcc
     1501 ggatcggtga aagccatact cgccctgccc ccgcgttcca ctatcccttc atgcggcacg
     1561 tccaggctgg taaaacgccc ctcccaccag tcgagcgcct cgcggcccgg tactctcaac
     1621 gctatcgccg tgatgctgcc cgcgcctgga ctgtctttgc ccgcgtgcgg ccagtcgaaa
     1681 aacgtaacct cagtacccgc atgtcctgct tcgtcaccat aaaagaggtg gtacatcgag
     1741 acatcatcct ggttcaccgt cttcttcacc aggcgcaggc ccagcacctg cgtataaaac
     1801 cgcacgttct cagccgcccg cccggtgacc gccgaaacgt ggtgcaatcc agatagcttc
     1861 attttatttc tctccctatc caggtctata attgctatgc agtaatgccc gacatctatt
     1921 tcatactaat acatcctacc acaactcgtc tctatcacag aatcggggcc gcgcctgagc
     1981 gagagaagat gaggtctctg tatagaaatc agcggctttg ctgccgattt ctatacagag
     2041 acctcatcct aatgtaagag gagtagtaac aaaacagata ggtcaggcac ggtatttgag
     2101 ccaggggtga gggctaccga gggggcggcg tggtatgacc accgttttgc cgctgttgcc
     2161 gtctatatct tcgccgtgta tataacccag gatgccgcac ttctctaagg catcgtgcgc
     2221 gccgcgcatg gtgagctttg cgtcccagcc tgagatgcgc ttgccatgct cgcgcaggta
     2281 ctcgcggagc gtgtcgcgga tgacgctgcc cgtgtcgcct cttcccttgc catgcactat
     2341 ctccagcgca tacatctccg cgcccgccgc gccggacgac agcaggcggt tgtaccggcg
     2401 caccaccacg agtatagcgt ccgcgagaat atagccgtgc aggtcgaggg attctaggtg
     2461 catgattaga agacagaaat tagaagtcag aagtcagaaa ccagaaacgg gtaaggtttg
     2521 cattcaattt tgcagtatac gatcataatt ataacttctg atttctgatt tctaacttct
     2581 tcacccctat tgcagcccct gcaccagccc ggcatacttg tctacgagtt ggcgagcggc
     2641 gaggtcggta tcggcttcgg ctatgacgtg gaagagtggg cggtcagcgt cggggaggat
     2701 tagcgcccat tcgccgtggc ccaggttgat cttcacgccg tctacctgct tggtgccgct
     2761 atcgcggtac tgctcgttga ggatgcgcat gacgcggcct ttgtcttccc aacggcaggg
     2821 cactcggctg gacgcccagg cgtagtgcgg tagggagcgg agcacctcgg tgaagctccg
     2881 gccctgctgg gcggtcatct ccatcagctt ggctatggcg aagaggccgt ccgccgaggg
     2941 ttgaaactga gggaagatga aggagccttg cccgtcgccc gccatgacca cgccactcct
     3001 gagggcggcg gtcatcagcg cgtaggagtt gaagcgggtg cggattacct cggcgccgta
     3061 cttggccgct atatcctcga atatggaggc ggcatagacg gggactacca gcatgccgtc
     3121 gctgccgtgc tgggcgaggg caagctccga catggcagcc agggtcgcca taccggacac
     3181 acgctggccc tggtcgtcca cgaaatatat acgctcgcct cccgtgtcga ggcgcactcc
     3241 gaagcccgct ttgagcgtct gggtgatagc gcccaggcgg tccatagcct gctcgaactg
     3301 ctcaggggtc tggtagaggg atagctcttg cacggcggcg ttgatggcga ccacgtcggc
     3361 gtctagcttg gctaacaggt tgggcagcac gagagcggaa gacgagttag cgtagtctat
     3421 ggcaatgcgg ctgtatcgct catctccgtg ctgtacggcc tcacagttca gattcttcag
     3481 ataatcgctg ccgtatctct gctccacgtc gggcgcgtag tcaattcgcc ccacgtcgtc
     3541 caggtagacg cggcggaagt cttcacggaa aaagagacct tccactttgc gctccgtcgc
     3601 cttgtccagg tcgaggccgc gcttgtcgaa gaacttgatg tccaccacgc gagcgtcgaa
     3661 gggcgagagg cggacgtgta cgccgcccgc cgcgcttatg gtgcgcgtat aatgccgggc
     3721 gacggggatg ggcacgttgc ggaggtccaa cacgttgatg cccgccgatg gcaggcccgc
     3781 gatgagggcg cgcttgatca tgcggggggt gtaatggctg tcgcgattta cggtgacggt
     3841 ggtgcccttg gcgagcatgc cgccgtatgc cgcgcccaat ttagccgcga actccggcgt
     3901 caggtctacg ttgaccaagc cggtgacgcc ccaccgcccg aagaggttgc gcctgctctg
     3961 agcgccccat atgagggagg tggtgacggt tgcgccgctc tcgacctctt tcgagggcca
     4021 tatcttgacg ttggggtgga tgaccgcgcc cgtgccgatg atggtgccgt cgccgatgac
     4081 cgcgccctcg aaaatcatcg cctgggtctt gacgttgacc tggggctgga ggatggcccc
     4141 gcgcacctcg gcacgctcgc cgatgtagca gttgcgccat atgatagagc ggtccaccgt
     4201 agcgcggttg tctactaccg agaagtcgcg gatgacggcg gggccgcgca ccgtcgcacc
     4261 cgactttatc ttgacgccgg tgcccaggaa aacggggccg gagatgtggg cgtcgcgggc
     4321 tatctccacc tcttcctgca cccatatacc cgaccccggc tcggtctcct gggcgagctt
     4381 gggcaggttc acttcgccgc gcaggtagtc gccggaggct atcatgtaat cggggatgga
     4441 gccaacgtcg caccagtagc cgtcggccac atagccgtac atagggtcgc ccttctcaag
     4501 cagtatgggg aatagctggc tgctgaagtc gaagacctgt ccggcttcgt aataatcgaa
     4561 tatcttcggc tcaaggatgt agatgccggt gttcaccgtg tcggagaaga cctcacccca
     4621 gcttggcttc tcctggaact gcc
//
LOCUS       DASRWZ010000297         8423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_779243, whole genome shotgun sequence.
ACCESSION   DASRWZ010000297 DASRWZ010000000
VERSION     DASRWZ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779243"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..497)
                     /locus_tag="VFH60_04615"
     CDS             complement(138..497)
                     /locus_tag="VFH60_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET6313095.1"
                     /translation="MSDQAIDTCVFCKLAKGQLRADIVYEDNTVLAFKDIHPQAPVHV
                     LVIPREHITALWEVDESHIQVLGRLILAANDVADRLGVQETGYRIVINTGTEAGQTVD
                     HVHLHVLGGRKLSWPPG"
     gene            complement(556..2049)
                     /locus_tag="VFH60_04620"
     CDS             complement(556..2049)
                     /locus_tag="VFH60_04620"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET6313096.1"
                     /translation="MQIRNADLVDGRYRIAQETAIRGFPQDSPPVRLYEGKDEQLERP
                     VTLQVLSADEARDPFQLQRFVSHGQLAAALHHQHVAGVYDAGEWNGWPYWVMARMGDT
                     SADSLYDEQHATPNVAAALAATRRAAEALQAVRAGGMADWTFSHQAVRISSSGDACLY
                     PIMGLEKLHGMDAHFTSSASQDDPRALSGLLRLLLVGTPDPRAAGTRMLALPPHVTAL
                     LDRMYLDGAPDKLTGAGEVASAIAELEASAGQPTQAYDPDQPPQPAPGPAVAMPYYGP
                     AEPSPVAPTALAAFAPAAIPASTPDAPTLVAPVPPPQSTRATAPVQARPYVPAPQPPQ
                     SPPTRRSSVLPLLPLLALLLLAGVGVVLWPRTNTASGSVAAGATVAPPTAQAQLVVVP
                     DLRGKTLEEANGAIGLAGLHLGTTEPVRDPAFEADTVARQVPDPGTPLQPGSVVTITL
                     SLGPQIATSPPQPAADPNPQPQEPPDKEKKKDKDEDKDKEKDKDKDD"
     gene            complement(2059..3573)
                     /locus_tag="VFH60_04625"
     CDS             complement(2059..3573)
                     /locus_tag="VFH60_04625"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6313097.1"
                     /translation="MGDPTQFHDRIQGMSIGGGTNIYSGLQPAVQALVASKARSKHIV
                     VLTDGVSEGGDYEGLLKQMEANNITLSSVAVGSDADEAFLQNLAIAGKGRFYSTEKGD
                     ALPQIFAHESHIAARSYLIEHPFAPARTSPSAILDGLGGLPELQGYVGTSPKPTGQIA
                     LVSDAGDPVLAQWQYGLGRVVAWTSDAKGQWATDWVTWPDFPRFWTQALRWSTGAEAG
                     NMLQPRVEWEGGTARITVDAAQPDGSLIGDLDVGAAVVGPNFVTSTVALRQTAAGRYE
                     GTFSATEEGAYLVQVRGTGATVGSVGQTLGMVVPYSPEYRGVPSDNGLLGRLASLTGG
                     RVLSLDNPAASLEHNLPAARSTSDLWPLLLFLAILLLPFDVGVRRLAITRADVARARA
                     ELQRRLGWDRRPAHATPQASTPQVAALFGAKERSRERFDAGSPPAQLPDLPPSPAPTA
                     ASARDLQLEGRTGRSTSAAQPPTSPQAVPEPTPQSGTEEDNMAARLRRSREQRH"
     gene            complement(3577..5004)
                     /locus_tag="VFH60_04630"
     CDS             complement(3577..5004)
                     /locus_tag="VFH60_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6313098.1"
                     /translation="MIGELGLSFSRPVALLLLLLLVPLTVYLSRTSLAFLRRGARRWS
                     LGLRLAIVVLLVLSLAGTGIVRADQRLSVVYLLDRSDSVSAEAQAQQAEYVRQAIADL
                     REGDQAGVIVFGSDALVDRPVLPDKAAPDLASAPSTTYSNLADALRLGLAVAPGDTAR
                     RLVLLSDGRENLGSAEWAARLAGANGVPVDVVSLPSASGPEVWVESLRAPSPVRENER
                     VSLQLAISSSVDTTATLLISVDGTALSTQEVRLVRGTNSYVQTLPPATRGFHTYSAQV
                     VAPAGVDTRSENNRYSAYSLVLGKPRVLIVEGKAGEAAALRAAIAPSVDSDLVPPFSM
                     PTGAAGLAGYDGIVLVNVPASSLTKTGMEALQVVVRDLGKGLLVVGGDESYAAGGYFR
                     TPLEDMLPVDLNLPSKLDIPSVGMVLVIDRSGSMDMGHVLNGVGVTKLSLAKEAAYQA
                     VAQLSERDYVGVIGFDSAATWAVEL"
     gene            5124..5459
                     /locus_tag="VFH60_04635"
     CDS             5124..5459
                     /locus_tag="VFH60_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313099.1"
                     /translation="MSVILANLGKSWRKVLVWAVVLGALATLIADLGEWDRFAVNLLW
                     RVLITAVGGVVGVVLDYFASFYVRMGTNIIESMNITSFSDDRQQKILNAFIFMMSLVA
                     AIIAMVFIQ"
     gene            5604..6422
                     /locus_tag="VFH60_04640"
     CDS             5604..6422
                     /locus_tag="VFH60_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313100.1"
                     /translation="MNGDLSQLVTLAAYGNVYLAREGADPPELVGHNPMFNFTSLKFV
                     RDPRPHGDGMRGTLVAESTAGWYTGLRELGARSIWLVRRGDSTSEDMPRHALVAFAGG
                     GDWHLQVDYRDKSEVWSANWHFVGKPGSGHWNVTYAAVPLAETPQVEKHDLAEVGTRL
                     RQALVEIEQFARANAEETWADIFKAARDELDGRGEARRAGMMPDEGYSSEAHSLLSAA
                     SRAWVFGGMGSWNDVYFPGEEARADYNRLTAQLYDAVVTAAVAATNSFERPLWS"
     gene            6413..6886
                     /gene="tsaA"
                     /locus_tag="VFH60_04645"
     CDS             6413..6886
                     /gene="tsaA"
                     /locus_tag="VFH60_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018670806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HET6313101.1"
                     /translation="MELDGAGAVTMQPIGVVRSPVVEGVDEDWGSVVAEVHLREEYAP
                     GLRGLEEFSHVIVVFLMHRATFSPGDDLVRRPRGRADMPELGIFAQRAKHRPNPIGLT
                     AVRLLSVRGTVLTVRGLDAIDGSPVLDIKPYFPAFDRVEDATTPEWVGRLMEGYF"
     gene            complement(6890..7105)
                     /locus_tag="VFH60_04650"
     CDS             complement(6890..7105)
                     /locus_tag="VFH60_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313102.1"
                     /translation="MSFVDWASALVLLLVAVAGLYGAGQAFLRRTSAYDGSQLGTRAS
                     ALLGLLYLAGGLVAGAILLLLVTQRLS"
     gene            complement(7102..7665)
                     /locus_tag="VFH60_04655"
     CDS             complement(7102..7665)
                     /locus_tag="VFH60_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-dependent phosphatase-1"
                     /protein_id="HET6313103.1"
                     /translation="MKLLAFDGDNTLWEPLDTVNVSDRTPTDAEGWPHFAFAPADDNP
                     LIATRDDGARFALRPEAREVLQTLKARDVLLAVVSYNHEGNIRRILEAFGVLGYFDYV
                     VAEFHSNKDRMLDRVLREAQADGHELSAADLMLVDDDPDNLYRGQCARMGAAFSCFGT
                     DIHDLREVLPLLDTPPQQPSAPGSHNR"
     gene            complement(7832..8035)
                     /gene="infA"
                     /locus_tag="VFH60_04660"
     CDS             complement(7832..8035)
                     /gene="infA"
                     /locus_tag="VFH60_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET6313104.1"
                     /translation="MITMDGVVADALPNAIFHVELENGHKVLGHISGKMRTNYIRIVP
                     GDRVLVELSPYDLTKGRITFRYR"
BASE COUNT         1404 a         2562 c         2971 g         1486 t
ORIGIN      
        1 tatacagtga gcaatcaagg ataagagtat agcatgtggg tcggtaagcc tcgcccttgt
       61 catttctcac tgcgttcgaa atgacaaggg cgaggctttg gtgctgatgt tggatatgct
      121 tattcttgag gggacttcta accgggaggc cagctcagtt tgcggccgcc gaggacgtgc
      181 aggtgtacgt ggtccaccgt ctgcccggcc tcggtgcccg tgttgatgac gatgcggtag
      241 ccggtctcct gcacgcccag gcggtcggcg acgtcgttgg cggccaggat gaggcggccc
      301 agcacctgta tgtgcgactc gtccacttcc cacagggcgg tgatatgctc gcgtgggatg
      361 acaagcacgt gcacaggggc ctgcgggtgg atgtccttga aggccagcac cgtgttgtcc
      421 tcgtagacaa tatcggcccg tagctggccc ttcgccagct tgcagaacac gcacgtatct
      481 atcgcctggt cgctcatagc ccctccggtc gcaaatctac accgggatta tacatgcaag
      541 ctcccgcccg gccaatcagt catccttgtc cttatctttt tccttgtctt tgtcttcgtc
      601 cttgtccttc tttttctcct tatcgggcgg ctcctgcggt tgcggattgg ggtcggcggc
      661 gggctgaggc ggggaggttg cgatctgagg ccccaggctc agggtgatgg tgacgacgct
      721 gccgggctgt agcggcgtgc cggggtcggg cacctgtcgg gccacggtgt cggcttcgaa
      781 ggcgggatcg cggaccggct ccgttgtgcc gaggtgcagc cctgccagcc ctatcgcgcc
      841 gttggcctcc tccagcgtct tgccgcgcaa atcggggacc acgacgagct gcgcctgggc
      901 agtaggcggg gcaaccgtag cgccggctgc cacggagccg ctggcggtgt tcgtcctggg
      961 ccacagcacc actcccaccc ccgcgagcag caacagggcc agcaacggca gcaagggaag
     1021 cacgctcgac cgccgcgtgg gtggactttg cggaggctgt ggcgcgggta catagggccg
     1081 cgcctgaacg ggagcggtgg ccctggtgct ctggggcggc ggcacgggag ccacgagagt
     1141 gggcgcgtcc ggcgtggaag cagggatggc ggccggcgcg aaagcagcaa gcgcggtggg
     1201 ggcgaccggc gatggctcgg cggggccgta gtaaggcatc gcgaccgccg ggccgggcgc
     1261 gggctgcgga ggctggtcgg ggtcgtatgc ctgcgtgggt tgtcccgcgc tagcctctag
     1321 ctcggcaatg gcagaggcta cttcccccgc gccggtaagc ttgtcgggag cgccgtccag
     1381 gtacatccgg tctagcaggg cggttacgtg cgggggcagg gcgagcatgc gcgtcccggc
     1441 agcgcgaggg tcaggcgtgc ccacgagcag gaggcgcagc aggccgctaa gggcgcgcgg
     1501 gtcgtcctgg gacgcggagg aggtgaagtg ggcgtccatg ccgtgcaact tctccaaccc
     1561 catgatgggg tacagacagg cgtcgccaga ggaggaaatg cgtacggcct ggtgggagaa
     1621 ggtccaatcg gccatgccgc cagcacgcac ggcttggagg gcttcggctg cgcggcgcgt
     1681 ggcggcgagg gcggcagcta cgttgggcgt ggcgtgctgc tcgtcgtaca ggctgtctgc
     1741 ggaggtatcg cccatgcgcg ccatgaccca gtagggccag ccattccact ccccggcgtc
     1801 gtacacgccc gccacgtgtt ggtggtgcag ggcggcggcc aactgcccgt ggctcacgaa
     1861 gcgttgcagt tggaaagggt cgcgggcctc gtcggcggag agcacctgca acgtgaccgg
     1921 ccgctcaagc tgctcgtcct tgccctcata aaggcggacc gggggactat cctgcgggaa
     1981 gccccgaatc gccgtttctt gcgctatcct gtatcggccg tccacgagat cggcattgcg
     2041 aatttgcata caacctgcct agtgtcgctg ctctctcgac ctcctgagcc gtgcggccat
     2101 gttatcttct tcggtgcccg actgtggagt tggctccggc acggcctgtg gggaggttgg
     2161 aggttgagcg gcggaagtgg agcgcccagt acgaccctca agttgcaagt ctcgtgccga
     2221 agcagcagta ggcgcggggg atgggggcag gtcgggcagt tgggcgggtg gggagccggc
     2281 gtcgaaccgc tcccggctgc gctctttcgc gccgaacagg gcggcgacct gcggggtgct
     2341 tgcctggggc gtggcgtgcg cggggcggcg gtcccagcca agcctacgct gcagctccgc
     2401 ccgcgcccgg gccacgtcgg ccctggtaat cgccagcctg cgcacgccca cgtcgaaggg
     2461 cagcagcagg atcgccagga acaggagcag aggccacagg tcgctggtac ttcgggcggc
     2521 gggcagattg tgctccaacg acgcggcggg gttgtcgagc gagagcacgc ggcccccggt
     2581 gagggaggcc agcctgccta gcagcccgtt atcgctcggc acgccccggt attcggggga
     2641 gtaggggacc accataccga gcgtctgccc cacgctgccc acggtcgcgc cggtgccgcg
     2701 cacctgcacc aaatatgcgc cctcctcggt ggccgagaag gtgccctcgt aacggccagc
     2761 ggcggtttgc ctcagcgcga ccgtggaggt gacgaagttg gggccgacca ccgccgcgcc
     2821 cacgtccagg tcgccaatga gggagccgtc aggctgggcg gcgtccaccg tgatgcgggc
     2881 ggtgccaccc tcccactcga cgcgaggttg gagcatgttg cccgcctcgg ccccggtgct
     2941 ccagcggagg gcctgcgtcc agaagcgggg gaaatcgggc cacgtcaccc agtcggtcgc
     3001 ccactgcccc ttggcgtcgg aggtccaggc caccaccctg ccgaggccgt actgccactg
     3061 ggccagcacg gggtcgcccg cgtcgctcac cagggcgatt tggccggtgg gcttggggga
     3121 ggtgccgacg tagccctgca attcgggcag gccacccagg ccgtcgagta tagcggaggg
     3181 agaggtgcgg gcgggggcga aggggtgctc tatcaggtag gagcgggcgg caatgtggct
     3241 ttcgtgggcg aatatctgcg gcagagcatc gcccttttcg gtggagtaga agcggccctt
     3301 gcccgcgata gccaggtttt gcaggaaagc ctcgtccgcg tccgagccga ccgccacgct
     3361 ggacagggtg atgttgttgg cctccatctg cttgaggagg ccctcatagt cgccgccctc
     3421 ggaaacgcca tcggtgagca cgacgatatg cttgctgcgg gccttgctgg cgacgagggc
     3481 ctgcacggcg ggctgcaagc cggagtagat gttggtgccc ccaccgatgg acatgccctg
     3541 gatgcggtcg tggaattgag tggggtcgcc caggggctac aactcgacgg cccaggtggc
     3601 cgcgctgtcg aagccaatca cgccgacgta gtccctctcg ctgagctggg ccacggcctg
     3661 gtaagcggct tctttggcca gcgagagctt ggtcacgccc acgccgttga gcacgtggcc
     3721 catgtccatc gagccggagc ggtctatcac cagcaccata ccgacgctgg ggatgtcgag
     3781 cttggagggc aggttgaggt ccaccggcag catatcctca aggggggtgc ggaagtagcc
     3841 cccggcggcg tagctctcat cgcccccgac caccagcagc cccttgccga ggtcgcgcac
     3901 caccacctgc aaggcctcca tgccggtctt tgtgagggaa gaggcgggca cgttcaccag
     3961 tactataccg tcgtagcccg caagccccgc cgcgcctgtg ggcatggaaa aggggggcac
     4021 caggtcggag tccacagagg gagcaatggc cgcgcgcaac gccgccgcct cgcccgcctt
     4081 gccctccacg atcagcacgc ggggcttgcc gagcacgagg gagtaggcgc tgtagcggtt
     4141 gttctcggag cgggtgtcca ccccggcggg ggccacgacc tgcgccgagt aggtgtgaaa
     4201 gcccctggtg gcgggaggga gggtctgcac gtagctgttg gtgccgcgca ccaggcgtac
     4261 ttcctgggtg ctaagggccg tgccgtccac ggagatgagc agggtagcgg tggtgtccac
     4321 gctggaggag atagcgagct ggagggacac gcgctcgttc tcccgcacgg gggaaggggc
     4381 gcgcaggctc tcaacccata cctccgggcc gctcgcggag ggcagggaca ccacgtccac
     4441 cggcaccccg ttagccccgg ccaggcgtgc cgcccattca gcgctaccca ggttctcccg
     4501 gccgtcggag aggagcacga ggcggcgggc ggtgtcgcca ggggccacgg caagccccag
     4561 gcgcaacgca tccgcgaggt tgctgtaggt ggtagagggg gcgctggcga ggtcgggggc
     4621 cgctttgtcg ggcagcacgg ggcggtccac cagggcatcc gagccgaata ctatgacgcc
     4681 tgcctggtcc ccttcgcgca ggtccgctat cgcctgcctc acgtactcgg cctgttgcgc
     4741 ctgcgcttcg gcggatacgc tatccgagcg gtcgagcagg tagaccaccg acagtcgctg
     4801 gtcggcccgc acgatgccgg tgcctgcgag cgacaatacg agcaatacca caatcgccag
     4861 ccgcagcccc aggctccacc gccgcgcccc gcgccgcaag aaagccaggc tggtgcgcga
     4921 caggtacaca gtcagcggca ccagcagcaa cagcaacagc agcgcgaccg gacgcgagaa
     4981 cgacagccca agttccccaa tcatgagtca ttatagcgga ttcaccattt tagattttag
     5041 gttttggatt ttggattagt agaagttctt ccaaatccaa aatctaaatc caaaatccgc
     5101 tatactggtc cgggggtgcg agcatgagtg tgatattggc gaacctgggg aagtcgtggc
     5161 ggaaggtgtt ggtttgggcg gtggtgctgg gggcactcgc cacgctgatc gcggacttgg
     5221 gcgagtggga ccgatttgcg gtgaacctgc tctggcgggt gctcatcacg gcggtgggcg
     5281 gtgttgtggg cgtggtgctc gactacttcg ccagcttcta cgtgcgcatg ggcactaaca
     5341 tcatcgagag catgaacatc acctcgttca gcgacgacag gcagcagaag atactcaacg
     5401 ccttcatctt catgatgtcg ctggtggcgg cgattatcgc gatggtcttt attcagtaat
     5461 gagtgatgag gactgaggac tgaggactgg gtaccgagcc acaccagtcc taaagcttgt
     5521 cttttcttgg cgcatgctac gtgtttttgt gcccactcag tactcagtcc tcatcactca
     5581 ttacttccct tggggcttat agcatgaacg gcgacctgtc acaactggtt acgctggcgg
     5641 catatggcaa cgtctacctg gcgcgggagg gtgccgaccc gcctgagctg gtggggcaca
     5701 accccatgtt caacttcacc tccttgaagt tcgtgcgcga cccaagacct cacggggacg
     5761 gcatgagggg cacgttggtg gcggagagca cggctggctg gtacacgggg cttcgagagc
     5821 ttggggcgcg cagcatctgg ctggtgcgtc ggggcgactc cacgagcgaa gatatgcccc
     5881 ggcacgcgct ggtggccttc gcgggcgggg gcgactggca tttgcaggtg gactaccgcg
     5941 acaagagcga ggtatggtct gccaactggc acttcgtagg caagccggga agcgggcact
     6001 ggaacgtcac ttacgctgcc gtgcccctgg ccgagacgcc gcaggtggag aaacacgacc
     6061 tggccgaggt ggggacgcgg ctgcgccagg cgctggtgga gatagagcaa tttgccaggg
     6121 cgaacgccga ggagacctgg gccgatattt tcaaggccgc gcgggacgag cttgacggcc
     6181 gtggggaagc ccggcgtgcc ggcatgatgc cggacgaggg ctacagcagc gaggcgcaca
     6241 gcctgctatc cgccgcctca agggcgtggg tgttcggggg tatgggctcg tggaacgatg
     6301 tgtacttccc cggcgaggaa gcccgcgccg attacaacag gcttacagcc cagctctatg
     6361 acgcggtggt cacggccgcc gttgccgcca ccaacagctt cgagaggccg ctgtggagct
     6421 agacggcgcg ggcgcggtga ctatgcagcc tattggtgtg gtgcgctccc cggttgtgga
     6481 gggggtggac gaggactggg gcagcgtcgt ggccgaggta caccttcggg aggaatacgc
     6541 ccccggcctg cgcgggcttg aagagttctc gcacgttatc gtggtgttcc tcatgcaccg
     6601 ggccaccttc agccccggcg acgacctcgt acgccgccct cgcggacgag ccgacatgcc
     6661 ggagctaggt atcttcgcgc agcgcgccaa acaccgcccc aaccccatcg gcctgaccgc
     6721 cgtgcgcctg ctgtcggtgc gggggaccgt gctcaccgtg cggggcctcg atgccattga
     6781 cggcagcccg gtgctggaca tcaagccata cttcccggcg ttcgaccggg ttgaggatgc
     6841 tacaacgccg gagtgggttg gtaggctgat ggaggggtat ttctagggct taggagaggc
     6901 gttgcgtgac caacagcagt aagatcgccc ccgccactaa gccgcccgcc aggtagagca
     6961 gccccagcag cgccgaggca cgggtgccta gctgtgagcc atcgtaggcc gacgtgcggc
     7021 ggaggaacgc ctgccccgcc ccgtacaagc ccgccaccgc cacgagcagg agcaccagtg
     7081 cgcttgccca gtctacgaag ctcatcgatt gtggctccca ggcgcggagg gttgctgcgg
     7141 aggggtgtcg aggaggggta gcacctcgcg caggtcgtgg atgtcggtgc cgaagcagga
     7201 gaaagccgca cccatacgag cgcattgtcc ccggtagagg ttgtcggggt cgtcgtccac
     7261 cagcatcagg tcggcggcgg atagctcgtg cccgtctgcc tgggcttcgc ggagcaccct
     7321 gtcgagcatg cggtctttgt tggagtggaa ttcggctacc acgtaatcga aatagccgag
     7381 cacgccaaag gcctccagta tgcgccgtat gttgccctcg tggttgtagc tcaccaccgc
     7441 cagcagcacg tcccgcgcct tgagcgtttg cagcacctca cgcgcttccg ggcggagggc
     7501 aaagcgcgcg ccatcgtccc gcgtagctat aaggggattg tcgtcagcgg gtgcgaaggc
     7561 gaaatggggc cagccctcgg cgtcggtggg agtgcggtcg gacacgttga ccgtgtccaa
     7621 cggctcccac aacgtattgt cgccgtcaaa ggctagcagc ttcatatatt cagtttggcg
     7681 cgaatgggag ggcttgtcct cggggcgggt gatttcatga aggtgaggca agcgtaaccg
     7741 gcgtgcgccg gtgggaaaga cagtaatact aggcctcacc ccgaaaagcg ggggagggcg
     7801 gtatgcaggc gtgcctttac ctgtagcggc cttacctgta gcggaaggta atgcggccct
     7861 tggtaaggtc gtagggcgat agctccacga gcacgcggtc gccgggcacg atcctgatgt
     7921 agttggtgcg catcttgccg gagatgtggc ccagcacctt gtgtccgttc tcaagctcga
     7981 cgtggaagat ggcgttaggc agggcgtccg ccaccacgcc gtccatcgtg atcacgtcgt
     8041 cgcgggtgcg gcgcggggct accggggcgg gtgtggcacc gggtgcggta ttggggcgct
     8101 gctggggcct cttggggtag ttctgtttct tcttcattcg gtaagccgtt tcctcctcgt
     8161 gcgtttccgc tgttgagcaa taaaatagcc ggagcgactt cccccggccc acctcacaac
     8221 gactatataa cggcacacgc agggcgtgta aggctatatc ggcaatctga gacggccaga
     8281 gaggtacatg tcggcgctcc ctctcctcgc ccaccggacg aggcgcagtg ctacgcctat
     8341 agtatacacg ctgcacgcct ggaatacaat gccgcttggt ggattttctg ggcgggtgtt
     8401 aagtgaattg gctcctctca gga
//
LOCUS       DASRWZ010000298         1906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_916460, whole genome shotgun sequence.
ACCESSION   DASRWZ010000298 DASRWZ010000000
VERSION     DASRWZ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916460"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..>1906)
                     /locus_tag="VFH60_04665"
     CDS             complement(38..>1906)
                     /locus_tag="VFH60_04665"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313105.1"
                     /translation="YTTINSGPRHAPFAAPGPLEQTAAALAGTYAPPRDLVSLTLRLK
                     LGGSGNVPQVVNSVPPSYTVGTRHEFNVANVTNRNYFTVTATVRYVTPHAYWYVRDGL
                     SVSMSALRASADRFEAKIYPKNRQVFGHEVSPGVDNDEHITVLITQVPGVGGYFAAAD
                     AFPRAVNPFSNQRDMIYMSTLPSGKSGDPGNYFEGTLAHEFQHMIQWNVHHNRDVWLD
                     EGASEIAMWVNGYDAGGVDYSFATNPDTQLNAWDEAGRTSAHYGASYMFLRYLMDRHG
                     GEGFMANILKHDLPGTTGLDAAIKEAGNPAGFEGAFRDWTIANVLNDRQLDGGRYSYS
                     QGGRVKPARTLSRYPATRTESVHQYAADYISLTGNSGPLTVSFKGSATVPVVAAKPHS
                     GQAYWYANRQDSADATLTREFDLTRASKATLQFWTWYDIEPAFDYAYVEASTDGGKSW
                     ATLKGRYTSEDNPNGNSFGSAWSGKSGLAAASKATPKWVQESVDLGAYAGKKVLLRFE
                     YVTDEGYNRPGFLVDDITIPEIGYSDSAETDGGWTAVGFVRIGSQLPQTWFVALIERG
                     SPNRVRVIPVDANGNGNITLSGLGRGTPTRDAILVISPQAPKTTEQAKYTVTVKKK"
BASE COUNT          274 a          578 c          676 g          378 t
ORIGIN      
        1 tggcgcgcgt cgccttacga gtaacatttc agggccgtta tttcttcttc accgtcaccg
       61 tgtacttggc ctgctcggtg gtcttcgggg cttgcggaga gattaccagg atggcatcgc
      121 gggtcggggt gccacggccg aggccggaga gggtgatgtt gccgttgccg ttggcatcga
      181 cgggtatcac gcgcacccgg ttggggctgc ctcgctcgat gagggctacg aaccaggtct
      241 ggggtagctg gctgccgatg cgcacgaagc ctacggccgt ccagccgccg tcggtctcgg
      301 cgctgtcgct gtagccgatt tcggggatgg tgatgtcgtc caccaggaag ccggggcggt
      361 tgtagccctc gtcggtcacg tactcgaagc ggagcagcac cttcttgccc gcgtaagccc
      421 ccaggtccac cgactcctgc acccacttgg gcgtggcctt cgacgcagcc gccagcccgc
      481 tcttgccaga ccaggccgag ccgaagctgt tgccgttcgg gttatcctcg gaggtgtacc
      541 tgcctttcag cgtggcccac gacttcccgc cgtcggttga ggcctctacg taggcgtagt
      601 cgaatgcagg ctcgatatcg taccatgtcc agaattgcag ggtggccttg ctcgcccgcg
      661 tcaggtcgaa ctcgcgtgtc agcgtggcgt ccgccgagtc ctggcggttg gcgtaccagt
      721 aggcctgccc gctgtggggt tttgcggcca ccacgggcac ggtcgcgctg cccttgaacg
      781 ataccgtgag ggggccgctg ttgccggtga gggagatata gtcggcggca tactggtgga
      841 ccgactcggt gcgcgtagcg gggtagcggc tgagcgtgcg cgctggcttc accctgccgc
      901 cctggctgta gctgtagcgg cccccgtcga gttgcctgtc gttgagcacg ttggctatcg
      961 tccagtcgcg gaacgccccc tcgaaaccgg ccgggttgcc cgcctctttg atagcggcat
     1021 ccaggccggt ggtgccgggc aggtcgtgct tgaggatgtt agccatgaag ccctcgccgc
     1081 catgcctgtc catcaggtag cgcaggaaca tgtaggacgc cccgtagtgc gccgaggtgc
     1141 gccccgcctc gtcccaggcg ttgagctgag tgtcgggatt ggtcgcgaag gagtagtcca
     1201 cgccgccggc gtcgtagccg ttgacccaca tcgcgatctc ggacgcgccc tcgtccagcc
     1261 agacgtcgcg gttgtgatgc acgttccact ggatcatgtg ctggaactcg tgggccaggg
     1321 tgccctcaaa gtagttgcca gggtcgccgc tcttgccgga gggcagggtg gacatgtaga
     1381 tcatgtcgcg ctggttgctg aagggattga ccgccctggg gaaggcgtcc gcggccgcga
     1441 aatagccgcc cacgcctggc acctgggtga tcagcaccgt gatgtgctcg tcgttgtcca
     1501 cgcccggcga gacttcgtgg ccgaacacct ggcggttctt ggggtaaatc ttcgcttcga
     1561 agcggtccgc cgaggcgcgc agggccgaca tgctcacact caggccgtca cgcacgtacc
     1621 agtaggcatg tggggtgaca tatcgcacgg tggcggtgac cgtgaagtag ttgcggttgg
     1681 tcacgttggc gacgttgaac tcgtgccgcg tgcccaccgt atagctgggc gggacgctgt
     1741 tcacgacctg gggcacgttg ccgctgccgc cgagcttgag gcgtagggtc agggagacga
     1801 ggtcgcgggg aggcgcgtag gtgcctgcaa gggccgccgc tgtctgctcg agggggccag
     1861 gggcggcgaa aggagcatgt cgcgggccgc tgttgatcgt ggtgta
//
LOCUS       DASRWZ010000299         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_710611, whole genome shotgun sequence.
ACCESSION   DASRWZ010000299 DASRWZ010000000
VERSION     DASRWZ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710611"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFH60_04670"
     CDS             <1..405
                     /locus_tag="VFH60_04670"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET6313106.1"
                     /translation="TIIVMGGIGGRLDHSVANLLLLTHPQLAGHDVRLVDGAQVAMLA
                     KPGRLNELPASPGDTVSLLPVGGDAEGVSTKDLLYPLHGETLLVGRGRGVSNEVTGPS
                     PSVDYQSGLLMVVIVRSGRSTEEQYGNSNEPR"
     gene            380..1210
                     /locus_tag="VFH60_04675"
     CDS             380..1210
                     /locus_tag="VFH60_04675"
                     /inference="COORDINATES: protein motif:HMM:NF021348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET6313107.1"
                     /translation="MAIQTNPDSGYRPQPAPTPTILMVVAGLAFLALFAGWITLYGWL
                     AQTVSGDTVWLGLLTAFFWLLVFVVVLVAARASNTVFASWQTRDILLLALVAAAFGPL
                     FVQWQVAYNAVFRWLGVGWSDMAQGFWFLPAILVPYIVRRPGAALFAETLAAAVSVLA
                     GSPYGIIGAAVGGLVQGMGAEVIFMLTGWRRYDWLTLSLAGAASAITGFIYYVPIYYA
                     GMDITLLAITGVSFVVGVMLLAVIPGKLLGDALLGTGVLDRFQIGRDRRASYATAEDF
                     "
     gene            1210..>2875
                     /locus_tag="VFH60_04680"
     CDS             1210..>2875
                     /locus_tag="VFH60_04680"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313108.1"
                     /translation="MPDSPGAPPIAVEGLRLRYPQEGRVVLRGVDLTLRRGEIKLLLG
                     PSGAGKSSLALTLNGLVPHQMEGDLRGRVLVNGTPTTETTPAALTSQVGMLFQDPESQ
                     IATLTVADEVAFGMENLRVPPEDIGPRVAAALGQVGLSGLEATGTGALSGGQKQRLAL
                     ASTLAMGVSILVLDEPTANLDPAGTSDFFKLLSRLKAGGISVLIIEHKLDELVEQVDS
                     IAVLGWEGKIVLDGAARQVLEEGRELLEELGVWLPQVSELANRLLDRGVTLQPYPLTV
                     DEAVATFGALAASFGPAPLVEAGAADQSAPPTLISVDSLSYRYQEGGFALRNASIRVR
                     EGDFYALLGPNGSGKTTLAKHLVGLLRPQAGSLTLAGENLKRIPPARLSREVAYVFQN
                     PEHQFVALTVFEELAYSLRAAKLPEAEVKTRAQRLIEEFGLGQHRQSNPFTLSQGQKR
                     RLSVATMLALDPRVLILDEPTFGQDNENATRIMKELQRLNDRGVTLLMITHAMKLVGE
                     YARRVGVLVRGEVRYEGSVEGLFENAALVAEARLELPPVYRVSEGVR"
BASE COUNT          479 a          886 c          966 g          544 t
ORIGIN      
        1 accatcatag tgatgggagg aatcgggggg cggctggacc actcggtcgc gaacctgctg
       61 ctcctcacgc acccgcagct tgccgggcac gacgtgcggt tggtggacgg cgcgcaggta
      121 gctatgctcg ccaagccggg ccggttgaac gagcttccgg cctctccggg ggacactgtt
      181 agcttgcttc cggtgggcgg ggatgccgag ggggtaagca ccaaagacct gttgtaccct
      241 ctgcacggcg agacgctcct ggtcggacga ggccgagggg tgagcaacga ggtgacaggc
      301 cccagcccaa gcgtcgatta ccagagcggg ttgctcatgg tggttatcgt ccgttcggga
      361 cgttctacgg aggaacagta tggcaattca aacgaacccc gatagcggct acaggccgca
      421 gcccgccccc acgccaacca ttttgatggt ggttgcgggg ctggctttcc tggcgctctt
      481 cgcggggtgg ataacacttt acgggtggct ggcccagacc gtgagcggcg acaccgtgtg
      541 gctcggcctg ctgacggcct tcttctggct gctggtgttc gtcgtcgtgc tggtggccgc
      601 ccgcgcgagc aacaccgtct tcgctagctg gcaaacgcgc gacattctgc tgctggcact
      661 cgttgccgcc gctttcggac cattgttcgt gcagtggcag gtcgcctaca atgccgtctt
      721 ccgttggctg ggcgtcggtt ggagtgatat ggcccagggc ttttggttcc tgcccgcgat
      781 actcgtcccg tacatcgtcc gcagacccgg cgccgccctt tttgccgaga cgcttgccgc
      841 ggcggtgtcg gtgctggcgg gtagccccta tggcatcatc ggcgcggctg tcggcgggct
      901 tgttcagggc atgggagctg aggtgatatt catgctcacc ggctggcggc gttacgattg
      961 gttgactctc tcgctggccg gtgccgcctc cgcgattact gggttcatct actacgtgcc
     1021 tatctactac gcgggtatgg acatcacatt gctggcgatt acgggcgtct cgttcgtggt
     1081 tggggtgatg ctgttggcgg taatccccgg caaactactt ggcgatgccc tgctaggcac
     1141 cggtgtgctc gaccgcttcc agattggccg cgaccgcaga gcaagctacg ccaccgccga
     1201 ggacttctag tgccggactc cccaggagcg ccgcccatag ccgtcgaggg gctgcgcctg
     1261 cgctaccccc aggaaggcag ggtggtgctc cggggtgtcg atctgacctt gcggcgtggc
     1321 gagatcaagt tgctgctggg gccgagcgga gccgggaaga gcagcctggc cctcaccctg
     1381 aacgggctgg tccctcacca gatggaaggc gacctgcggg ggcgtgtcct ggtcaatggc
     1441 acgccaacaa ccgagaccac gccggccgcg ctgacctcgc aggtcggcat gctgttccag
     1501 gaccctgaat cgcagattgc caccctcacc gtcgcggacg aggtagcgtt cggcatggag
     1561 aacctgcggg tgccgccgga ggacataggt ccacgggttg cggcagcgct ggggcaggtg
     1621 ggcctgtccg ggcttgaggc gaccggcacg ggtgcgctca gtggcggcca gaagcagcga
     1681 ctcgccctcg cctctacact cgcgatgggc gtgtcaatcc tggtgctgga tgagccgacc
     1741 gccaacctgg accccgccgg tacgtctgac ttcttcaagc tgctctcccg cctcaaggct
     1801 ggcggcattt cggtgctcat catcgagcac aaattggacg agttggtcga gcaggtcgat
     1861 agcatagcag tcttgggctg ggaggggaag atcgtcctcg atggggcggc gcgtcaggtg
     1921 ctcgaagaag gccgcgagct gcttgaggag cttggcgtct ggctcccaca agtgtccgag
     1981 ttggcaaacc gcctcctgga taggggagtc acattgcagc cctacccgct gaccgtagat
     2041 gaagcagtcg ccacctttgg tgctctggcc gctagcttcg gccccgcgcc tcttgttgaa
     2101 gccggggcgg cagaccaatc ggccccacct accctcatca gcgtggactc tctgtcatac
     2161 cgctaccagg aaggcggctt tgccctgcga aacgccagca tccgcgtgcg agagggcgac
     2221 ttctacgcgc ttctggggcc aaacggttcc ggcaagacaa cgctggcgaa gcacctggtc
     2281 ggcctcctgc gcccccaggc aggctcgctc acgctggcgg gcgagaacct caaacgcata
     2341 ccgcccgccc gcctcagccg tgaagtggcc tatgtcttcc agaacccaga gcaccaattc
     2401 gtggcgctaa ccgtgtttga agaactcgcg tactccctgc gcgcggcgaa gctacccgaa
     2461 gcagaggtca agacaagggc gcaacggctt atcgaggagt tcgggctggg gcagcacagg
     2521 cagagcaacc ccttcacatt gagccagggc cagaagcgcc gcctaagcgt ggctaccatg
     2581 ctagcgctcg accccagggt gttgatcctc gatgagccga cattcgggca ggacaacgaa
     2641 aatgccaccc gcattatgaa ggagcttcag cggctaaacg accgaggcgt gaccctcctc
     2701 atgatcactc acgctatgaa gctggtcggt gagtacgccc ggcgcgtggg tgtgctggtg
     2761 cgcggggaag tccggtatga gggcagtgtt gaggggctgt tcgagaatgc agcgttggtg
     2821 gcggaggcgc ggcttgaatt gccgccggtc taccgcgtct ccgagggagt gcgcg
//
LOCUS       DASRWZ010000300         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_848063, whole genome shotgun sequence.
ACCESSION   DASRWZ010000300 DASRWZ010000000
VERSION     DASRWZ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848063"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..1561
                     /locus_tag="VFH60_04685"
     CDS             443..1561
                     /locus_tag="VFH60_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6313109.1"
                     /translation="MSKQILITGGAGFIGSHLADELLARGYRVRALDNLTEQVHGPDR
                     RRPSYLSPDVELVVGDVRDRDMVRKALKGVDAVYHLAAAVGVGQSMYEIERYTQVNNI
                     GTAVLLEALAESPVERLVVASSMSIYGEGLYKAPNGSISEGRERDIEQLKARQWEVRN
                     AGGEVLTPVATPESKTPSLSSVYALSKYDQERLCLVVGRAYNSPTVALRFFNVYGTRQ
                     ALSNPYTGVLAIFASRFLSGQPPLINEDGYQQRDFVSVYDVARACRLALEVPEAAGQA
                     INVGSGQRYSVREIAARMAPLLHKEHLYPEITGKYRMGDIRHCFADISLARRVLGYEP
                     EVTLEEGLTELAGWLEKQIVVDYVPQAYAELATRGLTV"
     gene            1558..>2384
                     /locus_tag="VFH60_04690"
     CDS             1558..>2384
                     /locus_tag="VFH60_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6313110.1"
                     /translation="MKQHDSERPVTHNGSGMNGARKTAGNGHAGHEALVPAGVPVGTN
                     GPDDRPVLITGGSGFIGTNVAHRFLSEGRKVLVYDNLSRAGVKQNLDWLKATHGTNVE
                     AEIADIRDVVSLREAVSRASAVFHLAAQVAVTTSLEDPIEDVTVNARGTVNLLEALRV
                     LDSPPPLVFTSTNKVYGGLEDVEFRSNGHRYEPADPFIKMYGINERRRLDFHSPYGCS
                     KGAADQYVIDYARTYGLKAVVFRMSCIYGLHQFGTEDQGWVAHFLLRALHGEPITLYG
                     "
BASE COUNT          476 a          773 c          730 g          405 t
ORIGIN      
        1 gtggagcggc tgcatagcgg tggcgatagt atgaacagga ttgacaatta ttcaccgccc
       61 ataccagacg aattcacata cgttgattcc tagctggtat aaagattgct atgctatggc
      121 atgaacaaag aagtccttcc ggcatagaag tcgggacctt ctcatggtgc ggtctgacgc
      181 gctgtgaggc gtacgggagg cgcaacacct cggcaccgcg tcctctctag cagcttcgat
      241 tcgggcgtta caggacgttt ttacacgttc ctgtgtctgc aaacggtcac ccacctggca
      301 gagttgccat cagactgtgt gtttgctatg cccaaaaaca gggaatatac aaaacggtac
      361 ccgatatttc gcgtcctagc atgggcaact ccatacgtcc ctgtggaact acgcgcaaga
      421 aggacgagag ggagatgagc acgtgagcaa acaaatactg ataacgggcg gggccggttt
      481 catcggctcc cacctggccg acgagcttct ggctcggggc taccgagtgc gggcgcttga
      541 caacctcacc gagcaggtgc acggacccga ccggcgcagg ccctcctacc tcagccccga
      601 cgtggaactg gtggtggggg acgtgcgcga ccgcgacatg gtacgcaagg ccctcaaggg
      661 cgtggatgcg gtgtaccacc tggctgccgc cgtgggcgtg ggccagagca tgtacgaaat
      721 cgagcggtac acccaggtca acaacatcgg caccgccgtc ctgctggaag ccctggccga
      781 aagcccggtc gagcgtctcg tggtcgcctc aagcatgagc atctacggcg aggggctgta
      841 taaagcgccc aacggctcca tctccgaagg ccgcgagcgc gacatagagc agctcaaggc
      901 gcggcagtgg gaggtgcgca acgcaggcgg cgaagtgctc acccccgtcg ccacccccga
      961 atcgaagacc ccctccctgt cctccgtgta cgcactctcc aagtacgacc aggagcggct
     1021 gtgcctcgtg gtgggccgcg cctacaacag ccccaccgtg gccctgcgct tcttcaacgt
     1081 ctacggcacc cgccaggcgc tctccaaccc ctatacgggc gtgctggcga tcttcgcctc
     1141 ccgcttcctg agcggccaac cgcccctcat caacgaggac ggctaccagc agcgcgactt
     1201 cgtgagcgtg tacgacgtgg cccgcgcctg ccgcctcgcc ctggaagtgc cggaagccgc
     1261 cgggcaggcg atcaacgtgg gcagcgggca gcgctatagc gtgcgcgaga tagccgcgcg
     1321 catggccccc ctgctccata aagagcattt ataccctgaa atcaccggca aataccgcat
     1381 gggcgacatc cggcactgtt tcgccgacat ttcgctggcc cgccgcgtac tcggctacga
     1441 gcctgaggtc acgctcgaag agggcttgac ggagctggca ggctggctcg agaagcagat
     1501 tgtggtggac tacgtacccc aggcctatgc agaactcgcc acaagaggat tgacagtatg
     1561 aagcaacacg atagcgaaag gcccgtgacg cacaacgggt ctggtatgaa tggcgcgcgc
     1621 aagacggctg gcaacgggca cgccgggcat gaagcgctcg tgcccgcggg ggtacctgtc
     1681 ggcaccaacg gccccgacga ccgccccgtg ctgattacag gcggctcagg gttcatcggc
     1741 accaacgtgg cccaccgctt cctctccgaa gggcgcaagg tgctcgtgta tgacaacctc
     1801 tcccgcgccg gggtcaagca gaatctcgac tggctcaagg cgacgcacgg cactaacgtg
     1861 gaggccgaga ttgccgatat ccgcgacgtg gtgagcctgc gggaagccgt gtcgcgcgcc
     1921 tcggcggtgt tccatttagc cgcgcaggtg gcggtgacca ccagcctgga agaccccatc
     1981 gaggacgtca cggtcaacgc tcgcggcacg gtgaacctgc tggaagcctt gcgcgtcctg
     2041 gacagcccgc ctcccctggt gttcacctcc accaacaagg tgtatggcgg cctcgaggac
     2101 gtggagttcc gctcgaacgg ccaccgctac gagcctgccg accccttcat caagatgtac
     2161 ggcatcaacg agcgtcgccg cctcgacttt catagcccct atggctgctc caagggcgct
     2221 gccgaccagt acgtgatcga ctacgcccgc acgtatggcc tcaaagcggt ggtcttcagg
     2281 atgagttgca tctacggcct gcaccagttc ggcaccgagg accaggggtg ggtggcccac
     2341 ttcctcctgc gggcgctgca cggcgagcct atcaccctct acgg
//
LOCUS       DASRWZ010000301         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_230666, whole genome shotgun sequence.
ACCESSION   DASRWZ010000301 DASRWZ010000000
VERSION     DASRWZ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230666"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2640)
                     /locus_tag="VFH60_04695"
     CDS             complement(<1..>2640)
                     /locus_tag="VFH60_04695"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HET6313111.1"
                     /translation="TGALWAVGFYYMVLALRHRKWTDWLLSGLAWGFSLYFYAAGKMV
                     VPLAAALGLYCLVRWRLDFLKRYALGFALLGVTIVVTAMPYLIYSVETNFIYTMSRAE
                     ETSIFTPSNQAVTFLRFNLPYDPALAEGSTLQSLTQHPVEWARLLYEQARETLDVLYR
                     RADQVFFYLITDHGGSMFPAFWATIVMLGIAYATWKLWDSRYALVLIWFWFGLLGSIL
                     TVDTPNLQRVTGAWPALMLLPAALLDRIFAAGWPLSLSVARRWATIPLAAAVLFFGYT
                     SVQEYFGHYAATCPYCDSTAQARYAADLGQDYKAYQLGLGGYPYYFGHGSTRFLAKDV
                     EGVDLMAAADGLPVVDSNGKGLAFIVYYNNIEYLPLLKLYYPGGTEEPISSDDGVERF
                     RSYKVTREQLATFQVARATYRPSTGDPVTRDEPGLGTQRAQLVARQVKGQQLAPPQVM
                     EPWTPPAGATYPLQATWEGGLVAPNYGRYLFVAAGTATGVRLFVDGMEVIGEGAEPVA
                     PVEIVLAKGVHEVMLSGRLADTDTSLGLSWSGEGGENRPVETRFLYKGPKGGLTAEVG
                     PLAQPEAEALRLPDPFNGQQVRQRRVDPFIGFRETYNLFGSSPYLARWYGTLKADTAG
                     VYGFAATSPERVVLLIDDGIVIDGGNGRQSGSVQLTAGTHKIEMRYASPGGGARVELF
                     WNSPDGQAGFLPPTALVPAKRAWFESELLELQAPGTPVQPPQEAEPPPTTMRAPRAVL
                     GTQAGLKGPRGLAVDAAGNVYVGDNGSRRVAVFAPDGKVLSTWGRPLPETYKPEQGEA
                     PDGAFGEIFDLAVGVGADGQTYVYVLDSTTRIQVFATDGRQVGTYPAKQLDMFGPNGL
                     AIGPSGGEAGHKLYVSVTGENRL"
BASE COUNT          478 a          882 c          826 g          454 t
ORIGIN      
        1 ccagccggtt ctctccggta accgacacgt acagcttgtg cccagcctcg ccccccgacg
       61 gtcctatcgc caggccattc ggaccgaaca tgtcgagctg cttcgcggga taggtgccta
      121 cctgccggcc gtccgtggcg aacacctgga tcctggtggt actgtccagt acgtagacgt
      181 atgtctggcc gtccgcgccc actccaacag ccaggtcgaa aatctcgccg aacgcaccgt
      241 ccggggcttc gccctgttcg ggcttgtagg tctccggtag gggccttccc caggtgctta
      301 gtaccttgcc atcgggagcg aacaccgcca cgcgccggct gccgttgtcg cctacgtaca
      361 cgttgcccgc ggcatccacg gccaggcccc gcggcccctt aagcccggcc tgcgtgccca
      421 gcacggccct gggcgcgcgc atagtcgtgg gtgggggctc ggcctcctgt gggggctgca
      481 ccggggtgcc cggcgcctgc aactccagca gttccgactc gaaccaggcg cgctttgccg
      541 ggacgagcgc agtaggtggt aggaaaccgg cctgcccgtc cggcgagttc caaaatagct
      601 cgacgcgggc accgcccccg ggggaagcgt agcgcatctc tatcttgtga gtaccggctg
      661 taagctggac gctgccgctc tgccggccgt tcccaccgtc tatcacaatg ccgtcgtcta
      721 tcaacagcac gacgcgctca ggcgacgtcg cggcgaaacc gtacaccccg gcggtatcgg
      781 ccttgagggt gccgtaccag cgggcgagat acgggctact gccgaacaga ttataggtct
      841 ctcgaaagcc gatgaacggg tccacgcggc gctgcctcac ctgctggccg ttgaaggggt
      901 cgggcagcct gagcgcttcg gcctccggct gggcaagcgg gccgacttcc gccgtaaggc
      961 cacctttagg gcctttatag aggaaccggg tttccacggg ccggttttcc ccaccctcgc
     1021 ccgaccacga caggcccagg ctggtatctg tgtcggccag tctgccgctc aacattacct
     1081 cgtgcacccc cttcgccagt acaatctcca ccggcgctac cggctccgcg ccctcaccta
     1141 tgacttccat gccatccaca aatagcctca cccccgtggc ggtgccggct gcgacgaaca
     1201 agtacctgcc atagttggga gcgaccagcc cgccttccca cgtggcctgt agtgggtaag
     1261 tcgcacccgc aggaggggtc cagggttcca tcacctgcgg cggtgccaac tgctgcccct
     1321 ttacttgtct ggccaccaac tgcgcgcgtt gcgtgcccaa ccccggttcg tcccgcgtta
     1381 ccgggtcgcc ggtggacggt cggtaagtgg ccctagccac ctggaacgtg gcaagctgct
     1441 cccgcgtcac cttgtacgac ctgaagcgct ccacgccgtc gtcgctactt atcggctcct
     1501 ccgtgccgcc ggggtagtac agcttcaaga gcggcaggta ttcgatgtta ttgtagtaaa
     1561 cgataaaagc caggccctta ccgttgctgt ccaccaccgg caaaccgtca gcggcggcca
     1621 tcaaatctac cccttctacg tccttggcga gaaatcgcgt gctgccgtgg ccaaagtagt
     1681 acgggtagcc acccaggccg agttggtacg ccttgtagtc ctgccccagg tccgcagcgt
     1741 agcgggcctg ggccgtggag tcacagtagg ggcaagtcgc ggcgtagtgg ccgaagtact
     1801 cctggacgga agtgtagcca aagaagagga cggcagccgc cagcgggatg gtggcccagc
     1861 ggcgcgccac agacaggctc aggggccacc cggcggcgaa tatgcggtcg aggagggcgg
     1921 cgggtagcag catgagcgcg ggccacgcgc ccgtgacgcg ctgcaagttc ggagtgtcga
     1981 ccgtgaggat cgagccgagc aacccgaacc agaaccatat caacaccagc gcgtaccggc
     2041 tatcccagag cttccaggtg gcatacgcga tgcccagcat cactattgtt gcccagaagg
     2101 ccgggaacat gctgccaccg tggtccgtta tgagatagaa gaacacctgg tccgccctgc
     2161 gatacagcac gtctagcgtt tcgcgggcct gctcgtagag caacctcgcc cactcgacgg
     2221 ggtgttgggt aagcgactgc agtgtggagc cttcggcgag cgccggatcg tacggcagat
     2281 tgaaccgcag gaaagtcacg gcctggttgg agggcgtgaa gatggaggtc tcctcggcgc
     2341 ggctcatggt gtagatgaag ttggtttcga cgctgtagat caggtaaggc atagccgtta
     2401 ccactatcgt gacgccaagc agcgcgaacc ctagcgcgta ccgcttgagg aagtcgagcc
     2461 gccagcgcac aaggcagtag aggcccaacg ccgccgcgag aggtacgacc atcttgcccg
     2521 ccgcgtagaa atagaggctg aagccccacg cgaggcccga gagcagccag tcggtccact
     2581 tgcggtggcg caacgccagc accatgtagt agaagcccac cgcccatagc gcgcccgtgg
//
LOCUS       DASRWZ010000302         4988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_94419,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000302 DASRWZ010000000
VERSION     DASRWZ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94419"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFH60_04700"
     CDS             complement(<1..276)
                     /locus_tag="VFH60_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313112.1"
                     /translation="MIARLRRNPIILGFLVLVLLGILAFGWWTISPLFIRTTLVEGDD
                     FAIPAAARQDDMAMPQAKSDTIVATGNFDRKDAVHYANGQALIVKQAD"
     gene            complement(580..2073)
                     /gene="pyrF"
                     /locus_tag="VFH60_04705"
     CDS             complement(580..2073)
                     /gene="pyrF"
                     /locus_tag="VFH60_04705"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET6313113.1"
                     /translation="MSVTTNLETTWANRVAQSIAQQNSLLCVGLDPVYEKLPAEYRTG
                     SVAEDIFAYNRLIIDRAAPYAAAFKPQYKCYSAEGEAGIAALRLTCAYIKERYPHIPV
                     ILDAKYADIGHVLARCAHEAFDLFGVDAVTAMPAPGKQALAPLFSRPGRGCFMVVRTS
                     NPGADELQDLQTASGDPLYVEITRRIAAWNAEGDNGNIGLVGPATEPAVLARIRQAAP
                     NLPILCPGVGAQGGDGDEAVAAGLDPNGAGLLINVSRAIMEAADPGEAAREWRDIMNA
                     ARSRRADEPHGTATSLIEDVIRQMYEIGAIRFEPVTLKSGLVSPYYNNLRVLASYPPL
                     LRKVATLMSDTMQAAGVRPDLLVGIAEAGIPLAVALSQHTGIPAGYVRSSAKQHGTKR
                     MVEGAWQPGATAVLVDDVVSDGASKLEVLGHLQEAGLQVRDIVVLVDRGQGGPELMSR
                     HGLNCHAAVNMDDVLSILLKAGHITPQHVEDSHRFMQEARALTERTA"
     gene            complement(2070..2558)
                     /locus_tag="VFH60_04710"
     CDS             complement(2070..2558)
                     /locus_tag="VFH60_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313114.1"
                     /translation="MCFIIAALIPGVAIPLIYSQFRPFPIELALASVVAGAIFLIAGY
                     ANYRQATSVYLVTSPEGIEFHTHGNMRDALWSEITSIGPVPSYNGYYVEGLIIRSKVA
                     PSGLPSAHQEQGWFHWSVPLQPFGWYWKATGLGEEIRRYAPHLLNIQQEQPSRTDMQE
                     HQ"
     gene            complement(2648..3784)
                     /locus_tag="VFH60_04715"
     CDS             complement(2648..3784)
                     /locus_tag="VFH60_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6313115.1"
                     /translation="MTTPFYDPNLSYQENYDLGPFGLFAAEPSEGERRALEVPESEWV
                     VVAGLRLRRPLGIPAGPLLNSKYTDAAFRWGYDLCHYKTVRSRFWPSHAAPNVLFVGT
                     TRPIPPGEIGRDTLVARPFLRDEPVDLATLSITNSFGMPAQEPDVWQADMQRAVRGAG
                     PGQGLVASVTGTRMPGEDDRAYIEDHARAAAMCAETGAHAIEVNLSCPNLGGHGLMCH
                     DPQAAALVCKAVKQGIRHIPLFAKLGNYTPDERGEATLRHVVEATAPYVQGYGAVNAV
                     PVPVVGEDGGQALPGEGRGLAGVCGAALKATGLDVVRRLSRIRGVGGYDFAIIGVGGL
                     ASPADYRAYLEAGADAVQGATGPMWNYRLAVEIAVASRLQSAVV"
     gene            complement(3833..4810)
                     /locus_tag="VFH60_04720"
     CDS             complement(3833..4810)
                     /locus_tag="VFH60_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET6313116.1"
                     /translation="METPHLGYSYFAPAVMGAVQPGLSKAAHVAKVKAAMLRAAQDTL
                     DREGFTQVVPSMLTTTCGACGDPGTLVPVDMGGRQAYLRQTSQLHLEPLMRELGRVYS
                     ISRSFRAERRIDDRHLAEFTLLEGEAAGWDLQRTMGFMEGLVVAMLQQASTSAGQHLA
                     ALGAASTGKVKAPFARMTYDEAIIALQKRGHFAEWGEDLTNSHEIALTEMVGGPLFVT
                     HYPIGTRFFTMKVRRDDPRVVECCDLLMPGVGEVMGASETEHDPDILQTRLEASPSVR
                     QWLDLGGSTGDYDWYLDMHRHESLQQAGFGLGFERLVRYACCLKSVAEA"
BASE COUNT          841 a         1544 c         1649 g          954 t
ORIGIN      
        1 atcggcctgc ttgacgatga gagcctgtcc gttggcgtag tgcaccgcgt ctttccggtc
       61 gaagttgccc gtcgctacga tagtgtcgct cttagcttgg ggcatcgcca tatcgtcttg
      121 cctggcagcc gccggtatag cgaaatcgtc accttcaacc agggtcgtgc ggatgaagag
      181 gggtgagatg gtccaccaac cgaaggcgag gatacccagg agtaccaaca caaggaagcc
      241 caagataatg gggttgcgcc tcagacgtgc aatcacttct tcgctctcct cggggctatt
      301 cagggtttcg atgtcgtgcc ggcctggccc ctggtgcaat tatgcctcac ggagattacg
      361 tgaatattac aaagtctgcg gcattgttaa cgtcatgtga cgaaactgct gggcattcga
      421 aaagacgaaa acgccaagac gccaaggacc actccaagat aataaaggtt tggagagtag
      481 caagaggagc agggtacatg atgtcgtacc ctgctccttt tgcttagaac accatcctct
      541 atcttggcgt ggtccttggc atcttggcgt cttggcgttt caagccgttc gttcggtaag
      601 cgccctggct tcttgcatga agcggtggct gtcttctacg tgttgtgggg tgatgtggcc
      661 cgcttttagg aggatgctta gtacgtcgtc catgttgact gcggcgtggc agttgaggcc
      721 gtggcgggac atgagttccg gcccgccctg gccccggtct accagcacca cgatgtcgcg
      781 cacctgtagc cccgcctctt gcaggtggcc cagcacctcc agcttgctcg ccccgtctga
      841 taccacgtcg tccacaagca cggcggtagc ccccggctgc cacgccccct cgaccatgcg
      901 ctttgtgccg tgctgcttgg cgctggagcg cacgtagccc gccgggatgc cggtatgctg
      961 cgagagggcc acggccagcg ggatgcccgc ctccgcgata cccacaagga ggtcgggccg
     1021 cacgcctgcc gcctgcatcg tatcggacat gagcgtggct actttgcgca agaggggagg
     1081 gtaggaggcg agcacgcgca ggttgttgta gtaaggcgac accaggcccg acttgagcgt
     1141 gaccggctcg aagcgtatcg cgcctatctc gtacatctgc cggataacgt cctctatcag
     1201 gctggtcgcc gtgccgtgtg gctcgtctgc gcgccggctg cgtgcggcgt tcatgatgtc
     1261 gcgccactcg cgggcggctt ctccggggtc ggcggcctcc ataatggcgc gggacacgtt
     1321 gatcagcagc cccgcgccgt tcgggtccag cccggctgcc accgcctcgt caccgtcgcc
     1381 gccctgcgct cccacgccgg ggcagaggat gggcaggttg ggggccgcct gcctgatgcg
     1441 agccagcacg gcgggttccg tggccggccc caccaggccg atgttgccgt tgtcgccctc
     1501 cgcgttccag gctgctatcc tgcgggtgat ctccacgtag agggggtcgc cgctcgcggt
     1561 ctgcaagtcc tgcaactcgt cagcgccggg gttggaggtg cgcaccacca tgaagcaccc
     1621 tctgccgggg cgggagaaga ggggggccag ggcctgcttg cctggggcgg gcatggcggt
     1681 cacggcatcc acgccgaaca ggtcgaaggc ttcgtgggcg cagcgtgcaa gcacgtggcc
     1741 tatgtcggca tacttggcgt cgaggatgac agggatgtgg gggtagcgtt ccttgatgta
     1801 ggcgcaggtg aggcgcaggg ccgcgatacc cgcctcgccc tcggccgagt agcacttgta
     1861 ctgcggcttg aaagcggcgg cgtaaggggc cgcgcggtcg atgatgaggc gattgtaggc
     1921 gaatatgtcc tccgctacgc tgccggtgcg gtactcggcg ggcagcttct cgtacacggg
     1981 gtcgaggccc acgcagagga ggctgttttg ctgggcgatg gattgggcga cgcggttggc
     2041 ccaggtggtt tcgaggttgg tggttacgct cactggtgct cctgcatgtc ggttcttgag
     2101 ggttgttctt gctggatatt cagcaggtgc ggggcatagc gccttatttc ttcgccaagc
     2161 ccggttgcct tccagtacca gccaaacggc tgtaatggaa cagaccagtg aaaccagcct
     2221 tgctcttgat gggctgatgg aagtcccgaa ggcgcgacct ttgacctgat tataaggcct
     2281 tcgacatagt agccattata ggatggaacc gggccgatgc tcgttatctc cgaccacagc
     2341 gcgtcccgca tattgccgtg tgtatgaaac tcgatgcctt caggcgacgt taccaggtaa
     2401 acgctggtgg cctgtctata gtttgcgtag cctgcaatga ggaaaatagc gcctgctacg
     2461 acggaggcaa gggccagttc aattgggaaa ggccggaatt gggagtagat aaggggtata
     2521 gctacgccgg gaatcaaggc ggcaattatg aagcacacgt aagccacacg tcgcatcacg
     2581 agaagcgctg taggtctgaa ctcacgtcta ctacggctag cgtctgacac ggacgcattc
     2641 atatattcta caccaccgcg gactgtagcc tgcttgccac cgctatctcc accgccagcc
     2701 tgtagttcca catcggcccg gtagccccct gcacggcatc cgcccccgct tccaggtagg
     2761 cgcggtagtc ggcgggagag gccaggccgc ccacgcctat gatggcaaag tcgtagccgc
     2821 ctacgccgcg tatccgcgac aggcggcgga cgacatccag gccggtggct ttgagggccg
     2881 ccccgcacac gcccgcaagc cctctgccct cgcccggtag cgcctgcccg ccgtcctcgc
     2941 ccactacagg cacgggcacc gcgttcacgg caccgtagcc ctgcacgtag ggggcggtag
     3001 cttccaccac gtggcgcagg gtggcctctc cccgctcgtc gggggtgtag ttgccgagct
     3061 tggcgaacaa cggtatatgc ctgatgccct gcttcacggc tttgcagacg agggcggcgg
     3121 cctgggggtc gtggcacatc aggccgtggc cgccgaggtt cgggcacgac aggttcacct
     3181 caatcgcgtg ggcacccgtt tccgcgcaca tggcggcggc ccgcgcgtgg tcctcgatgt
     3241 aggcccggtc gtcctcgccc ggcatgcgcg tgcctgtgac gctggctacc agcccctgac
     3301 ctggccctgc gccgcgcacc gcacgctgca tgtcggcctg ccacacgtcc ggctcctgcg
     3361 cgggcatgcc gaaggagttg gtgatgctca gggtggcgag gtccaccggc tcgtcgcgca
     3421 ggaaggggcg ggcaaccagg gtgtcgcgcc cgatctcgcc cggggggatg ggccgggtcg
     3481 tgcccacgaa gagcacgttg ggggcggcgt gcgagggcca gaagcggctg cgcaccgtct
     3541 tgtagtggca caggtcgtag ccccagcgga aggcggcgtc cgtatacttc gagttgagca
     3601 gcggccccgc cggtatgccc agcggcctgc gcagtctcag ccccgctacg accacccact
     3661 cgctctccgg cacctcaagc gcccgccgct ccccctccga aggctcggcc gcgaacagcc
     3721 cgaacggtcc gaggtcgtag ttctcctggt acgagagatt agggtcgtag aatggtgtgg
     3781 tcatggcatc tagataataa cacgtaacgt gttacgcgtt acgcgttact cctcacgctt
     3841 cagcgaccga cttcaggcaa caggcgtagc ggaccaggcg ctcgaagccg aggccgaagc
     3901 cggcttgctg gaggctttcg tggcggtgca tatcgaggta ccagtcgtaa tcgccggtgc
     3961 tgccgccgag gtctagccac tggcgcacgc tgggggaggc ttcgaggcgg gtttgcaaga
     4021 tgtcggggtc gtgctcggtc tcggaggccc ccatgacctc gcccacgccg ggcataagca
     4081 ggtcgcaaca ctctaccacg cgggggtcgt cgcggcgcac cttcatcgtg aagaagcggg
     4141 tgccgatggg gtaatgcgtc acgaacagcg ggccgcctac catctcggtg agggcaattt
     4201 cgtggctgtt agtgaggtct tcgccccatt cagcgaagtg gcccctcttc tgcaaggcga
     4261 ttatagcctc gtcgtaggtc atgcgggcga acggggcctt caccttgccg gtgcttgccg
     4321 ctcccagggc cgcaaggtgc tggcccgcgg acgtacttgc ttgttgcagc atggcgacca
     4381 ccaggccctc cataaagccc atcgtgcgtt gcaggtccca gcctgccgcc tcgccctcaa
     4441 gcagggtgaa ctcggccagg tggcggtcgt ctatgcgtcg ctcggcgcga aaggagcggc
     4501 tgatagagta gaccctgcca agctcgcgca taaggggttc gaggtggagt tgggaggtct
     4561 gacgcaggta agcctgccga ccgcccatat cgacagggac gagggtgccg gggtcgccgc
     4621 acgcgccaca cgtcgtggtg agcatcgagg gcactacctg ggtgaatccc tcccggtcca
     4681 acgtgtcctg cgcggcccgt agcatggcgg cttttacttt ggctacatgg gcggccttgc
     4741 tcagtcccgg ctgcacggca cccatgacgg cgggtgcgaa gtagctatag ccgaggtggg
     4801 gggtctccat tgtctatcct ccttgcggag gcgcataccc acaaaaaaac cttgcacggg
     4861 gcaaggctgg tggtcgccgc gtgtgcggca gcatctatgg gcatacacca accttgacgg
     4921 cctcacggga ccggcattaa aggtcgttcc gagccgagcg cgacacgcga tgtgcctccg
     4981 gctccgct
//
LOCUS       DASRWZ010000303         4757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_642682, whole genome shotgun sequence.
ACCESSION   DASRWZ010000303 DASRWZ010000000
VERSION     DASRWZ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642682"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..2723
                     /gene="clpB"
                     /locus_tag="VFH60_04725"
     CDS             87..2723
                     /gene="clpB"
                     /locus_tag="VFH60_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HET6313117.1"
                     /translation="MNLQKFTEKAQEAVLTARQVAEDNGNSQIEPLHLLLALVQQRNG
                     VVPGVLTKLGVQPSALAGRVEEELARLPKVQGSGVQVYVSSALSDVARRAEKAAESMR
                     DEYVSAEHLLLAMSEDAEKGTAGKLLRESGITRDKTLSVLAQIRGNQRVTDQNPEAKY
                     AALEKYGRDLTELARQNKLDPVIGRDEEIRRLIQVLLRRTKNNPVLIGEPGVGKTAIA
                     EGLAVRIVRGDVPEGLKNRKLVALDLGAMVAGAKYRGEFEERLKAVLKEVVEARGEII
                     VFIDELHTVVGAGAAEGAMDASNMLKPMLARGELHAIGATTLDEYRKHIEKDAALERR
                     FQPVLVDEPTVEETISILRGLRERYEVHHGVRITDGALVAAAMLSNRYITDRFLPDKA
                     LDLVDEAAARLRMEAESMPVALDEVERRIMQLQIEREALRKEKDAASKERLERVNKEL
                     ANLEEQRSQLRARWQAERDSLNEVRAVKEQIEQANADLERAQRSTDYARASQIQYGTL
                     RELETRLQELEANDGKDGAQRLLKEEVDSDDIAAVVSRWTGVPVERMLEGELAKLLRI
                     EERLHLRVIGQDEAITAVANAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELARALAE
                     YLFDDENAMVRIDMSEYMEKFAVSRLIGAPPGYVGYEEGGQLSEPVRRRPYSVVLFDE
                     IEKAHPDVFNVLLQILDDGRLTDAQGRTVDFKNTIVIMTSNIGSQHIMDLGPDEEETM
                     RRRVNEALRSHFRPELLNRIDEIVIFHQLTREHLREIVDLQLNRVRGRLVERHITLDV
                     TSHALEFLGNEGFDPVYGARPLKRVIQRRLMDRLAVAMLDGTVRDGDTVHVDVRNGDV
                     AVSVRNPDDAKEPAGREAQAVS"
     gene            2785..3732
                     /locus_tag="VFH60_04730"
     CDS             2785..3732
                     /locus_tag="VFH60_04730"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET6313118.1"
                     /translation="METITPTPAPARTSKVTSVEEAIARLLPDVSESLWVGGMHMHNV
                     PMTLVRECIRQGKRFETLYAGPSASIAADLLIGAGLVERLVCGYIGYEHMGLAPVFRR
                     AVEERLDGVERAGLREVVEADSGSMVLALQAGASGQPFAALPTGLGNTSLPAASPSFY
                     RDVRDPFTGAETFAVEAVRPAVALIHCQQADEFGNGIFKGSPFTDRLLALAGRTVIMQ
                     VEQTVDNDQVLRYPAQTGVPGFLVSAVVPVTFGCHPTSSHRYYNYDDVHLRLYMQAAG
                     TREGFEQYLAEYVLQPEDQEEYMRRVQNQDWGAMRESDT"
     gene            3729..4562
                     /locus_tag="VFH60_04735"
     CDS             3729..4562
                     /locus_tag="VFH60_04735"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET6313119.1"
                     /translation="MSDDYKPVLRPGSDPLVFFPFPGADQPVEVAKGHVMACAIARAL
                     KDGELVVMGANSLLPMSAARLAQQTHAPNLTIIAGASGGVNTLVEPLVPSSGDYANLV
                     AEAVLQFHEVLMLQMSGHADVFFAGGLQIDRQGNCNLAYVGEAASPTLRGPGSAGVPW
                     AQQSARTILYTTSHTRRVFVPRVDFVSYPGWQTSASSPGRGGPQLVVTPLAVMDFSDG
                     GEMRLVSLHPGVSLEQVRENTGFDLVLPEGEVPTTLEPSALELRLLREMDPDALLTVV
                     V"
BASE COUNT          922 a         1410 c         1626 g          799 t
ORIGIN      
        1 cgtagcaaac ttgacaatga tacactcaac ttcctataat tgcgatggta tataagataa
       61 atgcgtatat aggagggggt gattatatga accttcagaa gtttacggag aaggcacaag
      121 aggcggtcct gaccgcgcgg caggtagccg aagacaacgg caatagccag atagaaccgt
      181 tgcacctgct gctcgcgctc gtccagcaga gaaacggggt cgtgcccggt gtgctcacca
      241 agctgggggt gcagccgtcc gcgctcgcgg gcagggttga agaagagctg gcgcgcctgc
      301 cgaaggtgca ggggtccggc gtgcaggtgt acgtttcgag cgcgttgagc gacgtggccc
      361 gccgcgccga gaaggccgcc gagtcgatgc gcgacgagta cgtcagcgcc gagcacctgc
      421 tgcttgccat gtcggaggat gccgagaagg gcaccgcggg caaactgctg cgggagtcgg
      481 gcatcacccg tgacaagacc ctttcggtgc tcgcccagat ccggggcaac cagcgcgtga
      541 ccgaccagaa cccggaggcc aagtacgcgg cgctggagaa gtacggccgc gacctcacgg
      601 aactcgcccg gcagaacaag ctcgatcccg taatcgggcg cgacgaggaa attcggcgct
      661 taatacaggt gctcttgcgc cgcaccaaga acaaccccgt gctgataggc gagccgggag
      721 tcggcaagac cgccattgcc gagggcctgg ccgtgcgcat cgtgcgcggg gacgtaccgg
      781 agggcctgaa gaaccgcaag ctggtggcgc tcgacctggg ggctatggtg gccggtgcca
      841 agtaccgggg cgagttcgag gagcgcctga aagcggtgct caaggaagtc gtggaggcgc
      901 gcggcgagat tatcgtgttc atcgacgagt tgcacacggt agtgggtgcc ggtgcggcag
      961 agggtgcgat ggacgcctcc aacatgctca agccgatgct ggcgaggggc gagcttcacg
     1021 cgattggcgc gaccacgctt gacgagtacc gcaagcacat cgagaaggac gcggcccttg
     1081 agcggcgttt ccagcccgtg ctggtggacg aacctaccgt ggaagaaacc atctccatcc
     1141 tgcggggcct gcgcgagcgg tacgaggtgc accacggcgt gcgcatcacc gacggcgcgt
     1201 tagtcgccgc tgccatgctc tccaaccgct acatcacgga ccgcttcctg ccggacaagg
     1261 ctctagacct cgtggatgag gcagccgccc ggctccgcat ggaggccgag agcatgccgg
     1321 tggccctgga cgaggtggag cgccggatca tgcagttgca gatagagcgc gaggcgttgc
     1381 gtaaggagaa ggacgcggcc agcaaggagc ggctggagcg ggtgaacaag gagcttgcta
     1441 acctggagga gcagcggtcg cagcttcgcg caaggtggca ggcggagcgc gactcgctga
     1501 acgaagtgcg cgcggtgaag gaacagatag agcaggcgaa cgcggacctg gagcgggcgc
     1561 aacgcagcac cgactacgca cgcgcctcgc agatccagta cggcacgctg agggagcttg
     1621 agacgcgcct gcaggagctt gaggccaacg acggcaaaga cggcgctcag cggctcctga
     1681 aagaggaagt agacagcgac gacatcgccg ccgtggtcag ccgctggacg ggagtgccgg
     1741 tcgagcgcat gctggagggc gagctggcca aactgcttcg catcgaggag cggctgcacc
     1801 tgcgcgtgat agggcaggac gaggcgatta cggccgtggc gaacgctgtg cgccgggcgc
     1861 gggcagggct gcaagacccc aaccgcccac tggggtcgtt cctgttcctg ggtcctaccg
     1921 gcgtgggcaa gacggagctt gcgcgtgcgc tagccgagta cctcttcgac gacgagaacg
     1981 ccatggtgcg tatcgatatg tcggagtaca tggagaagtt cgccgtatcg cggctgattg
     2041 gcgcgcctcc gggctacgtc ggctacgaag agggcgggca gctaagcgag ccggtgcgcc
     2101 gccgtcccta ctcggtggtg ctattcgatg agatagagaa ggcccacccc gacgtgttca
     2161 acgtgctgct gcaaatcctg gacgacggcc gcctgaccga cgcgcagggg cgcaccgtgg
     2221 acttcaagaa cacgatagtc atcatgacct ccaacatcgg ctcccagcat atcatggacc
     2281 tgggcccgga cgaggaggag acgatgcgcc gccgcgtgaa cgaggctctc aggtcgcact
     2341 tcaggccgga actgctgaac cgcatagacg agatcgttat cttccaccag cttacgcggg
     2401 agcacctgcg cgagatcgta gacctgcaac tcaaccgtgt acgcgggcgg cttgtcgagc
     2461 gacatatcac gctagatgtg acgtcgcatg cccttgagtt cctgggcaac gaaggcttcg
     2521 acccggtgta cggcgctcgc ccgctcaaga gggtaataca gcgcagactg atggaccggc
     2581 tggctgtggc gatgctcgac ggcacggtgc gcgacggcga cacagtacac gtggacgtcc
     2641 gcaacggcga cgtagctgtc tcagtccgga atcccgatga cgcgaaggaa ccggcgggca
     2701 gggaagccca ggccgtgtcg taacgaagct gaacgggcag ggtgcagttg gtgccctgcc
     2761 cgttctatta cagtgagtcg agctatggaa actatcactc ctaccccggc acccgcacgc
     2821 acgagtaaag tgacttcagt agaagaggct attgcacgcc tgctgccgga cgtcagcgag
     2881 tcgttgtggg taggcgggat gcacatgcac aacgtcccca tgacgctcgt acgggagtgc
     2941 atccggcagg gcaagcggtt cgagacgctt tatgccggcc cgtccgcctc cattgcggcc
     3001 gacttgctca ttggggctgg cctcgtagag cgcttggtgt gtggctacat cggctacgag
     3061 cacatggggc ttgcaccggt gttccggcgg gcggtcgagg agcgactaga cggcgtcgag
     3121 cgcgcgggtt tgcgcgaggt ggtggaagcc gactcgggga gcatggtgct ggcgttgcag
     3181 gctggggcgt cggggcagcc gttcgcagcc ctaccaacgg ggcttggcaa cactagcctg
     3241 cccgccgctt cgccttcgtt ctaccgggat gtgcgcgacc ctttcaccgg agctgagact
     3301 ttcgctgtcg aagcggtacg tcccgcagtc gcgctcatac attgccagca ggccgacgag
     3361 ttcggcaacg gcatcttcaa gggcagtccc tttaccgacc gcctgcttgc cctggcaggt
     3421 cgaacggtca tcatgcaggt cgagcaaacc gtggacaacg accaggtgct aaggtacccc
     3481 gcgcaaaccg gcgtgccagg cttcctggta tcggcagtgg tgccggtcac gttcggttgc
     3541 caccccactt cctcacaccg ctactacaat tacgacgacg tgcacctgcg cctctacatg
     3601 caggcggccg gaacgcggga aggcttcgag cagtacctcg ctgagtacgt gttgcagccg
     3661 gaggaccagg aggagtacat gcgtcgggtc cagaaccagg actggggcgc gatgagggag
     3721 tccgacacat gagcgacgac tacaagccgg tgctgcggcc aggctccgac ccgctggtct
     3781 tcttcccctt tccaggggcg gaccagcccg tcgaagtggc gaaggggcat gttatggcct
     3841 gcgctatagc acgtgccctg aaggatggcg agttggtggt gatgggtgca aacagcctgc
     3901 tcccaatgtc cgctgcccgg ctggcccagc aaacccacgc gcctaatcta actatcatcg
     3961 cgggggccag cggtggcgtg aacaccctcg tggagccgct ggtgccttcc tccggcgact
     4021 atgctaacct cgtggcggaa gcggtgctac agttccacga agtgctgatg ctgcaaatga
     4081 gcgggcatgc agacgtgttc ttcgccgggg gcctccagat tgaccggcag ggcaactgca
     4141 acctggccta cgtgggagag gcggcaagcc ccacactgcg cggccccggc tcggcggggg
     4201 tgccctgggc gcaacagtcg gcccgcacca tcctttacac cacctcgcac acgaggcgcg
     4261 ttttcgtgcc cagggtggac tttgttagct accctggctg gcaaacctcg gcaagcagtc
     4321 ccggccgggg tgggccccaa ctcgtggtga cgccgctggc cgttatggac ttcagcgacg
     4381 ggggcgagat gcggctggtg agcttgcacc ctggtgtgtc tttggagcag gtgcgcgaga
     4441 acaccggctt tgacctagta ctgcctgaag gggaagtccc cactacgctg gaaccgtcag
     4501 cgctggaact gcgtctcctg cgcgagatgg acccggacgc gctcttgacc gttgtggtat
     4561 agtcgcaggg cgttagcttg cgcatacttt gcaagagcct catgccgtgt tattctctgc
     4621 gcggcggccc tgacatttga gcctctgttt agtgaagatt agctgtttaa tcaggtaatg
     4681 gccaggcacg aatgggtgct acgtaggggc aggcccccgt gccagccctg gtggcaggcg
     4741 tacacaggtg ctaatat
//
LOCUS       DASRWZ010000304        11366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_642792, whole genome shotgun sequence.
ACCESSION   DASRWZ010000304 DASRWZ010000000
VERSION     DASRWZ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642792"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..444)
                     /gene="queF"
                     /locus_tag="VFH60_04740"
     CDS             complement(82..444)
                     /gene="queF"
                     /locus_tag="VFH60_04740"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HET6313120.1"
                     /translation="MPTKPSKDLETFPNPHPSNDYEIDIDCPEFTALCPMTGQPDFAT
                     IHINYKPGPRCVELKSLKLYIWSFRDEGHFHEDVTNRILNDLVAALDPKEITVTGDFG
                     VRGGIKTIVTARHGSRTA"
     gene            666..1928
                     /locus_tag="VFH60_04745"
     CDS             666..1928
                     /locus_tag="VFH60_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6313121.1"
                     /translation="MDTGDREQRGTQAGKWDNDTSEFLGQTSLFRGLPGERLSDVAAR
                     MEPRHYTRNQEILRQGSVTSEVYLVKRGIVAVTRDSTLTGESHTLAYLMEGALLGELE
                     FLSSVQGAATAAATATALTELDLLVLRGHDFLELLQSESSVALGLARELARRLRATNE
                     RISTGDGLENRVCLVIGNGRGAGVTTVGTALALSLAASTQDPTAYTEYPDAARLQAEL
                     GFVAGPRPYGHPGGFDAPIAPDTEEPFLPALRATLFFDQLALSYPNIVIGLTGEPDAS
                     LAYLLERTDQVVIVTSPDPAEAASLMTLVSRLKRLIHSDRSNLYIVSNRPAPGDAHLP
                     TPPYADFDVPNLVDLPAPAAMRAEGLPPALSSVAATLADRVKRTNQVRMYIPTTMDVD
                     RQIDTSLYVQRALDLLGDLFGGATSDDA"
     gene            1932..2114
                     /locus_tag="VFH60_04750"
     CDS             1932..2114
                     /locus_tag="VFH60_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313122.1"
                     /translation="MWKTDTEGLVGETVYIVTSYVTQTDLDRHLARIVDFVEGLKHEL
                     KQEAMALEVNEKLMLI"
     gene            complement(2127..3317)
                     /locus_tag="VFH60_04755"
     CDS             complement(2127..3317)
                     /locus_tag="VFH60_04755"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6313123.1"
                     /translation="MTAPLTVRDLTLPGEPLDGAQSLTGEIGLDNPVVWVVSLRPFSP
                     AFPRLRGGEIALVATENLLRHDPPITLPDVVRLLSSTGGAGVAVRGGVDAAAVRAAQE
                     AELALLSLPADAPLADLEQAIMRECAFHQARTEISRAEEPESWLEDLLSGRDELLEGA
                     VSRAERQGYPPSRQYALAFVLPRQTGGSGTAKSGEALANLSDAIREITLQRGNRPVAA
                     RYDGGLAVLLPGGSEGALDKALKSAGEWACGIGTARPLLQAGISLEEARLASTASALV
                     HEGAPTRFAELGPLRLLLLLHRDQPHELDRFVYESIGLLLRHDTGTANPLLPTVQAFI
                     EHGGRLRDTAEHLIVHRNTLAYRLRRAGEILGADLRDADVRLGLELALKALPLVRAAE
                     ANGQ"
     gene            complement(3632..5341)
                     /locus_tag="VFH60_04760"
     CDS             complement(3632..5341)
                     /locus_tag="VFH60_04760"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET6313124.1"
                     /translation="MKDILTREATTSTGDSDRGNGAGHHTPDDAGLTSGFSQPYSQRE
                     AVLLPPGRRRWRVALAALLAAAFIVAAIFVLASPSKGPATAQATRGTIISSVETTGKL
                     EAQKSARLSFRSSGRVERVLARQGDRVRPGQVLAELDSDNLQRQLREAQAHSHISELR
                     LQQAKLGPRQEDVTAATADLNAATAQLQQLKGGGRPEDVAAAQAALNQAQAQLDSLKK
                     GPSPQELTAAQARLDQATANRDLVAATASNATEQARILYVQAQSAAEDFLDPAGQIEQ
                     ARLNYEAAQKSETAQMASANARIREAQAGLDALRAGPSPEDVRAAEQGVAQAQAHLDR
                     VRNGASQQDIATAQARVDAAQANLDKLKAGATDSDISILEQQVALSQIDVERAQAQLA
                     DAQIASPMDGTLLSIDLEVGEIVNGSQPVAVVADTTSLRIKADVDEIDVGRVQAGQAV
                     TVTLDAYPGVRLPGKIESLAPGATLKQGSTVYQATVSFVPTGEVVPREGMAANVDITA
                     QRKDGVLLLPNRAFEAVGRRHYVTVRQGDTTARLEVEVGLSNNSDTEIISGLEEGQTV
                     LLK"
     gene            complement(5402..6868)
                     /locus_tag="VFH60_04765"
     CDS             complement(5402..6868)
                     /locus_tag="VFH60_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6313125.1"
                     /translation="MAAVFLFICLLLSTAGLASAQPLAQADGVTAQRNVTYCNVGGEA
                     LKMDLFLPKPGGGPAPVVVYVHGGSWVGGDKFEVGLAGNELARKGYVVASINYRLGPK
                     HKWPAQIQDAKCAVRYLRASAGQYNLDPNRIAAWGSSAGGHLAALLGLTDPSAGFDNS
                     GQYLNQSSRVQAVVDMFGPTDLNAYDPDKYSRGVGQAVFGVTRDQPESRELLRRASPV
                     TYAGSSPGVPFLILHGDKDSLVPLSQSQALYDRLKAAGTDAELVVVRNGEHGFFPTGG
                     EISPSTAQIKNLIDNFFDRTLGHTATAPRTFPETGRTVQGAFMRYWSANGGLTQFGYP
                     ISEEMQEVSDTDGKTYRVQYFERAVFEYHPDNPQPYDVLLSLLGSMRYAQKYPGGAPG
                     QVPNAAPGSILFAETGKRVGGLFLEYWKSNGGLARQGYPLSEEFKEVSSINGRTYTVQ
                     YFERAVFEWHPENRPPFQVLLSPLGRLHYEVKHGAEQP"
     gene            complement(7053..9401)
                     /locus_tag="VFH60_04770"
     CDS             complement(7053..9401)
                     /locus_tag="VFH60_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6313126.1"
                     /translation="MIISLRKAFRDLTRRRLRSFLTILGIIIGVAGLVAITSTSKNMT
                     AAQAAAYSNNSQQDMRWWVGNAPPGLENGVRQVPNIAEVELRANYFTKWYATGAWRDI
                     QFIGIRDFDNMRVNKVDLVEGRWPGRGEIVLESSVREVAPVVIGDEVVYRAGPGNAQH
                     DLTVVGFARSPSFPSANIIGTSIGYVPNTEVHKMYGEDGDNQLLVRLTDFSPGLRADT
                     KGEIQRVFEKRNLAYGSYFERNPDDYVGKRQLDALVLLMTVFSVVGLVISSFLVANTL
                     AAVISEQMAEIGAMKAIGATSGKVIGIYVIAGAMYGAVGTVLGLLIGVFGGHALLLYL
                     GTMFNLSIGGIRVDPLDIAQGIAVGVGVTTLAAFLPAWRGTSITVRKALESYGISATY
                     GQGWLDRIVQSFSQLPRIPAMAVRNLARRKGRNLITVGAIGLATAAFLGAQSTSVSVQ
                     LSIENAYDVYGSDAWVWFQQPVGQTFAADLRSMPEVTVAEAWASTSAFAGDLRVTMWG
                     MPASTTLYRPPFVQGRWFTDEEPFSVVVSSRFAELRGYEPGDRVEVTVGGTDVWLTIV
                     GTVNDNAQGLSSSSRGKMFAPLETVSRLMHRSGSADFFAVRFDRSDGPYVEETLARLE
                     RKFHDLQPGMLAAYADKEASLEASKILTILLYAMTVIVGTIGGIGIANTLTLNVLERR
                     REIGVMRSLGARDGHLIQVFLTEALIMGGGGFLLGLGLGYPLAVTLVWLMSTVLFPLD
                     FLFPASMVGLAMLFTLALTTVASIGPALGAAKLKVSHALRYE"
     gene            9710..11284
                     /locus_tag="VFH60_04775"
     CDS             9710..11284
                     /locus_tag="VFH60_04775"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HET6313127.1"
                     /translation="MFKMSSSPGAPRTQPLRRSTRLISILSLLLLLAFLLLLRETSLQ
                     AEVTHAAGLGSVPAQADRFGQKDGRKEAPPSVEQAGGRNSQVPQGACSQTQIDEGFDD
                     ITTLSASGWFTQNNSSPQGVTGWFQGNPEAFPAHSGAANSYVGANFNNTTGSNTISNW
                     LLSPQVSLQNGDMFVFWTRTSEDNPLPDRLEVRLSTAGASTNVGDTSTSVGDFTVLLR
                     SINPNLEVGGYPEVWTQFVVTLNVFSGTESGRIAFRYYVTDGGPAGDNSNYIGIDTVA
                     YYTGCSPQATPTATACPVQFNDVPSSSPFYANVRCMACRGVISGYGCGAADEPCPGSY
                     FRPGVNVTRAQISKMVAIAAGLDDEPTGARKFQDVPEGSTFYQWVQQLANSGAISGYA
                     CGGANPANGEAEPCVAPGNLAYFRPNNNTTRGQLTKIVSTAAALTGDPGSQKFADVPP
                     NGTFFEWVQRLSNRGVVGGYSCGGTNPATGAPEPCDAASRPYFRVNNNVTRGQIAKIA
                     AETFFPNCQTPARSIK"
BASE COUNT         2004 a         3652 c         3574 g         2136 t
ORIGIN      
        1 acgagattct tcgctgcgct cagaatgaca aagtggggac tttggtgctc ctgttcgaca
       61 ctggcactct tgagatagct gctaggcggt acggctgccg tgcctggcag tcacgattgt
      121 cttgatgccg cccctgacgc cgaagtcgcc tgtgacggtt atctctttag ggtcgagggc
      181 ggctacgagg tcgttgagga tgcggttggt aacgtcctcg tggaagtggc cttcgtcgcg
      241 gaaagaccat atgtacagct tcaacgattt cagctccacg caacgcggcc caggcttgta
      301 gttgatgtgt atggtagcga agtcgggctg cccggtcatc gggcacaggg ccgtgaactc
      361 cggacaatct atgtctatct cgtagtcatt gctggggtgc gggttaggga aagtctccag
      421 gtctttggat ggcttggtcg gcatgtttgg atctcgtttc caggtaatat tttcgcttgt
      481 gctatcggct gagggatggc atcttgcgcc aacccggcaa cctgaaatta taccacagtt
      541 cgggtaatgg ccgggcttcg tgtgaaggct gaagtgttgc tccaggcccg gcgtgcgctt
      601 tcgtgagacc tggcagcacg cgttctacac tggctgtgcg cgataaaggt tgggagggta
      661 ggcagttgga caccggcgat agggaacaac gaggtacgca ggctggcaaa tgggacaacg
      721 acacttccga gttcctgggc caaaccagct tgttccgggg gctgccgggc gagcgcctgt
      781 ccgacgtggc cgccagaatg gagccacggc actatacccg caaccaggag atattgcgcc
      841 agggtagcgt cacgagcgaa gtgtacctgg tcaagcgcgg catagtggcc gtgacccgcg
      901 actccacgct cacgggggag tcgcacaccc tggcgtacct gatggagggc gcgctgctcg
      961 gtgagctgga gttcctgtcg agcgtgcagg gggctgccac cgccgcggcg acggctacgg
     1021 ccctcaccga gctagacctc ctggtcttgc ggggccacga cttcctggaa ctgctacaga
     1081 gcgagagcag cgtggcgctt ggcctggccc gcgagctggc ccgacggttg agggccacca
     1141 acgagcgcat cagcacgggc gacgggctgg aaaacagggt gtgcctggtg ataggcaacg
     1201 gccgcggagc cggggtcacg acggttggta ccgccctggc cctgtcgctg gccgcctcta
     1261 cccaggaccc caccgcctac accgaatacc ctgatgccgc ccgcctccag gctgagcttg
     1321 gctttgtcgc ggggccgcgg ccttatgggc acccaggcgg gttcgacgct cccattgccc
     1381 ccgacaccga ggagccgttt ctacctgcac tccgcgctac cctctttttc gatcagcttg
     1441 cgctctccta ccctaacatc gtgatcggcc tcaccgggga gccggatgct tcgctggcct
     1501 acctactcga acgcaccgac caggtggtta tcgtgacctc gcccgacccc gccgaggccg
     1561 catcactcat gacgctggtg agtcgcctga aaaggctgat tcactcggac aggagcaacc
     1621 tctatatcgt cagcaatcgt cccgcgccgg gggacgccca tctgcccaca cccccttatg
     1681 ccgacttcga cgtgcccaac ctggtggact tgcccgcacc tgccgctatg cgggctgaag
     1741 gccttccccc agccctttcc tccgtagccg ccaccctggc cgaccgggtg aagcgtacca
     1801 accaggtcag gatgtacatc ccgaccacca tggacgttga ccggcaaatc gacaccagcc
     1861 tgtacgtgca gcgggcgctc gacttgttgg gcgacctctt tggcggggcc accagcgacg
     1921 acgcataggg cgtgtggaag accgacaccg agggcctggt gggcgagacg gtgtatatcg
     1981 tgaccagcta cgtcacacag acggaccttg accggcacct cgcccgcatc gtggacttcg
     2041 tggagggcct caaacacgaa ctgaagcagg aggcaatggc cctcgaggtc aacgagaagc
     2101 tgatgctcat ctaagggagc aagggcttac tgcccgtttg cctccgcagc tctgacaagc
     2161 gggagggcct tcaaagccag ttcgagcccc agcctgacat cggcatctcg caagtcggct
     2221 cccaggattt ctccggctcg ccgcaacctg taggccaggg tgttgcggtg cacgatcagg
     2281 tgctcggcgg tgtcgcgcag cctgccgcca tgctcgatga acgcctgcac ggtaggcaaa
     2341 agcgggttag ctgtgcctgt gtcatgcctc aataataagc caattgactc ataaacaaaa
     2401 cgatcaagct catgtggctg gtccctgtgc agcagcagga gcagcctgag cggtcccagc
     2461 tccgcgaacc gggtaggcgc tccctcgtgc accagcgcgg aagcagtgga ggcaagccgg
     2521 gcctcttcga gcgagatgcc cgcttgcaac agcggcctcg cagtgccgat accacaggcc
     2581 cactctcccg cgcttttcag ggctttgtcc aaagcgccct cgctaccccc aggcaacaac
     2641 actgccagcc ccccatcgta gcgagccgcg acgggcctgt tgccgcgctg caaggtgatt
     2701 tccctgatag catcgctcag gttggccagc gcctcgccgc tcttcgctgt gccgctgccg
     2761 cccgtttgcc tgggcaagac gaaggccagg gcgtactgtc tcgacggtgg gtagccctgc
     2821 ctctcggccc tgctgacggc cccttccagt agctcgtcac gccccgacag caggtcttca
     2881 agccagctct cgggttcctc ggcacgggag atctcggtgc gggcctggtg gaaagcgcac
     2941 tctcgcatga tggcctgttc gaggtccgcc aggggagcgt cggcggggag ggacagcagc
     3001 gccagctccg cctcttgagc cgcccggacc gccgccgcgt ccacgccacc gcgcaccgcc
     3061 accccggccc ccccggtcga ggagagcagc cgcaccacgt cgggcagggt aatgggggga
     3121 tcgtgccgca gcaggttctc ggtggcgacc agggctatct caccacccct gaggcgaggg
     3181 aaggccgggg agaaagggcg cagcgacacc acccacacga cagggttgtc gaggccaatc
     3241 tcgcccgtga gggactgcgc gccgtccaga ggctcgccgg gcagggtcag gtcgcgaacg
     3301 gtgagggggg ctgtcatgct atggcccggc tcaggccagg caggccaact cgtgtggctg
     3361 ccgcagtgcc tttggcgagc atcgtcctcc tcccccacgc ctacttggat ttggccctca
     3421 ggatagcaga tgccttgcac gcgcgcaagg gacgccctgc gatagccctg tgggggtgcc
     3481 ctgccaacac cgccctgcca atgaattggc gggcttacca gagacgaagt ctcctacgga
     3541 gactacctga tgttgcttag tcccctatgg gactttgcct cttctcagcc cgtggcttca
     3601 gccacagggc tatcgcaggg caagcatgct cctacttgag cagcaccgtc tggccttctt
     3661 ccaggccgga gatgatttcg gtgtcggagt tgttgctcag gcccacctcc acctccagcc
     3721 tggcggtcgt gtcgccctgg cggaccgtta cgtagtggcg gcgtcctacg gcctcgaagg
     3781 cgcgattggg gaggaggagc acgccgtcct tgcgctgggc cgtgatgtcc acgttggcag
     3841 ccataccctc tcggggcact acctcccctg tgggcacgaa cgatacggtc gcctggtaaa
     3901 cggtgctgcc ttgcttgagc gtggcccccg gtgccagcga ctcgatcttg cccggcagcc
     3961 tgacgccggg gtaagcatcg agcgtcacgg tcacggcctg gcccgcctgc acccgtccta
     4021 cgtcgatctc gtctacgtcg gcttttatgc gcagtgaggt ggtatcggcc accaccgcca
     4081 cgggctgcga gccgttgacg atctccccta cttcaaggtc gatgctcagc aaggtaccgt
     4141 ccatcggaga tgcgatttgc gcgtcggcaa gctgggcctg ggcgcgctcc acgtcaatct
     4201 gcgagagggc cacctgctgt tccagtatgg agatgtcgct gtcggtagcg cccgccttca
     4261 gcttgtcgag gttagcttgc gccgcatcca cgcgggcctg ggcggtggcg atgtcctgct
     4321 gggaggcccc gttacgcacc ctgtcaagat gggcctgcgc ctgcgccaca ccttgctcgg
     4381 cggcgcgaac gtcttcgggg gaaggcccgg ccctgagcgc gtccagcccg gcctgggctt
     4441 cccttatcct ggcgttggcc gaggccatct gagcggtctc gcttttctgc gcggcctcat
     4501 agttgaggcg ggcctgctct atctgccccg ccgggtcgag aaaatcttcg gctgcgctct
     4561 gtgcctggac gtagaggatg cgggcctgtt cggtggcgtt ggaagctgtg gcggccacga
     4621 ggtcgcggtt ggccgtcgcc tggtcgagcc gggcctgggc ggccgtaagc tcctggggcg
     4681 agggtccttt cttgagcgag tccaactgtg cctgggcctg gtttagcgcg gcctgggccg
     4741 ccgccacatc ctcgggacgc ccgccaccct taagctgctg tagctgggcc gtggcggcgt
     4801 tcaggtcggc ggtggcggcg gtcacgtcct cctggcgcgg gccgagtttc gcctgctgca
     4861 agcgcaactc gctgatgtgc gagtgcgcct gggcctccct gagttgccgt tggaggttgt
     4921 ccgagtcgag ctcggccagc acctgcccag gccttacacg gtccccctgc ctggcgagca
     4981 cccgctccac gcgccccgac gagcggaagg agaggcgagc gctcttctgc gcctccagct
     5041 tgcccgtcgt ctccacgctg gagatgatcg tgcccctggt cgcctgcgcg gtggccgggc
     5101 ctttggaggg tgaggccagg acgaaaatcg ccgcgacgat gaaggcggcc gccagcagcg
     5161 cagccagcgc tacccgccag cgcctgcgcc cgggaggtag cagcacggct tcgcgctggg
     5221 agtacggctg ggagaagccg ctcgtcagcc ccgcatcgtc gggggtatgg tggcccgcgc
     5281 cgttgccccg gtcgctatcg ccggtgctcg tggttgcctc tctggtaagg atgtctttca
     5341 taccgtcagc ttcctgtctg ctctatacgt tttcaccatc tataataccc cattgcgctc
     5401 cctatggttg ctctgccccg tgcttcacct cgtaatgcaa cctgcccagg ggggagagca
     5461 gcacctggaa gggcggccgg ttctcagggt gccactcgaa taccgcccgc tcgaagtact
     5521 gcaccgtgta cgtcctgccg tttatggacg agacttcctt gaactcctcg gacagagggt
     5581 agccctgccg cgcgaggccg ccgttggact tccagtactc caggaacagc ccgcccaccc
     5641 gcttgcccgt ctccgcgaac agtatcgagc cgggcgcggc gttcggcacc tgccccggcg
     5701 cgccaccggg gtacttctgc gcgtacctca tcgagccgag cagcgacagg agcacgtcgt
     5761 agggctgcgg attgtcgggg tggtactcga acacggcccg ctcgaagtac tgcaccctgt
     5821 aggtcttacc gtcggtgtcg ctgacttctt gcatttcttc cgaaatgggg tatccgaact
     5881 gcgtcaggcc accattcgcg gaccagtagc gcatgaaggc accctgtacc gtcctccccg
     5941 tttcggggaa ggtacgcggg gctgtagccg tatgtcccag cgtgcggtcg aagaaattat
     6001 ctatcaagtt cttgatctgg gcggtgctcg ggcttatctc tcccccggtt gggaagaagc
     6061 cgtgctcccc gttgcgcacc accacaagct ccgcgtccgt gcctgccgct ttcagacggt
     6121 cgtagagggc ctgcgactgg ctgagcggga cgaggctgtc cttgtcgccg tggaggatga
     6181 ggaaaggcac gccgggcgag ctacccgcgt aggtgacggg gctggcccgc cggagcagtt
     6241 cgcgcgactc cggctggtcg cgggtgacgc cgaacaccgc ctgccccacg cccctggagt
     6301 acttatcggg gtcgtaagcg ttgaggtcgg tcgggccgaa catgtctacc accgcctgca
     6361 cgcggctcga ctggttgagg tattggccgg agttgtcgaa gcccgccgag gggtcggtga
     6421 ggcccagtag agcggcgagg tggcccccgg cgctgctgcc ccacgcggcg atgcggttgg
     6481 ggtcgaggtt gtactgcccg gcgctggccc tcaggtagcg caccgcacac ttggcgtcct
     6541 gtatctgcgc gggccacttg tgcttcggcc ccagccggta gttgatagag gcgacgacgt
     6601 agcctttacg cgcaagctcg ttgcccgcca accccacctc gaacttgtcg ccgcccaccc
     6661 agctaccgcc gtggacgtag actaccacgg gggccggccc gccgcccggc ttcggcagaa
     6721 acaggtccat cttgagggct tccccgccca cgttgcagta ggtgacgttg cgttgcgcgg
     6781 tcacaccgtc tgcctgggcc agaggctgtg cgctcgcaag cccggcagtg ctgaggagca
     6841 ggcatatgaa gaggaaaacc gcagccattt tgcccggcgg ttgaaaccgc gagcaagccc
     6901 tacaaagtcc ccctacgggg actcccttgc atttgggaca gtccgcgcag gcggactttg
     6961 taaatattgc tcgcggtttc aaccgccggg catcccacat tagcatctca agtctcatca
     7021 ttaacaacta acaactaaca actaacaact cgtcactcat acctcagcgc atggctgacc
     7081 ttcagcttgg ccgcccccag ggccgggccg atgctcgcca ctgtagtaag cgccagggtg
     7141 aacaacatcg caagccctac catgctcgcc gggaagagga aatccagcgg aaacagcacc
     7201 gttgacatga gccacaccag cgtgacggcc aggggatagc ccagccctag ccccagcagg
     7261 aatccacccc cgcccatgat gagggcctcg gtgaggaaca cctgtatcag gtggccatcg
     7321 cgcgccccca gcgagcgcat cacccctatc tcgcgtcgcc tttcgagcac gttcagcgtg
     7381 agggtgttgg cgatgcctat gcccccgatg gtgcccacta ttacagtcat cgcgtagagg
     7441 aggatggtga gtatcttgct ggcttcgagg ctggcttctt tgtcggcgta ggcggcgagc
     7501 atgccgggct ggaggtcgtg gaacttgcgc tccaatcgcg ccagcgtctc ctcgacgtag
     7561 gggccgtccg agcggtcgaa gcgtacggcg aagaagtcgg cggacccgct ccggtgcatc
     7621 aggcgcgaca ccgtttccag cggcgcgaac atcttgcccc tggacgagga gcttagcccc
     7681 tgcgcgttgt cgttcacggt gcccacaatc gtcagccaca cgtccgtgcc gcccacggtc
     7741 acctctaccc tgtcgcccgg ctcgtagccc cgcagctcag cgaagcgcga cgagaccacc
     7801 accgaaaaag gctcctcgtc ggtgaaccag cggccctgta cgaagggcgg tcggtagagg
     7861 gtggtggagg cgggcatgcc ccacatggtc acccgcaggt ccccggcgaa cgcggaggtg
     7921 gaggcccagg cttcggccac cgtgacttcc ggcatcgagc gcaggtcggc ggcgaaggtc
     7981 tgccccaccg gctgctggaa ccacacccag gcgtccgagc cgtacacgtc gtaggcgttc
     8041 tctatcgaga gctgcaccga gacggaagtg ctctgcgcgc ccaggaaggc agccgttgcg
     8101 aggccgatag cccccaccgt aatcaggttg cggcccttac ggcgggccag gttgcgcacg
     8161 gccatagcgg ggattctcgg tagctggctg aagctctgca cgatacggtc gagccagccc
     8221 tggccgtacg tggcgctgat gccgtagctt tccagcgcct tgcgcactgt aatggaggtg
     8281 ccccgccagg cagggaggaa ggccgccagc gtggtcaccc ctacgcccac cgcgatgccc
     8341 tgcgcgatat cgaggggatc gacccggatg ccgccgatgc tcaggttgaa catggtgccc
     8401 aggtagagca gcagcgcgtg cccgccgaac acgcctatca gcagccccag cacggtgccc
     8461 accgccccgt acatcgcccc cgcgatgacg tagatgccta tcaccttgcc cgacgtggcc
     8521 ccgatggcct tcatcgcgcc tatctcggcc atctgctccg agatcaccgc cgccagcgta
     8581 ttcgccacca ggaaggacga tatcaccagg cccacgacgg aaaagaccgt catgagcagc
     8641 acgagcgcgt caagctggcg cttccccacg tagtcatcgg ggttgcgctc gaagtaggag
     8701 ccgtaggcca ggttgcgctt ctcgaagacg cgctgtatct cccctttggt gtcggcgcgc
     8761 aggccgggcg agaagtcggt gaggcgcacg agtagctggt tgtcgccgtc ctcgccgtac
     8821 atcttgtgca cctcggtgtt aggcacgtag ccgatggagg tgccgatgat gttggccgag
     8881 gggaaggacg gcgaccgcgc gaagcctacc acggtgaggt cgtgctgcgc gttgcccggc
     8941 ccagcccggt agaccacctc gtcccctatg acgacggggg cgacctcccg caccgaagac
     9001 tcaagcacaa tttcgccgcg ccccggccag cgcccctcca cgagatcgac cttgttgacg
     9061 cgcatgttgt cgaagtcgcg gatgccgatg aactggatgt cgcgccaggc cccggtagcg
     9121 taccacttgg tgaaatagtt ggcgcgaagc tctacctccg cgatgttggg cacttgcctg
     9181 acgccgtttt cgaggccggg cggggcgttg ccgacccacc agcgcatgtc ctgctgggag
     9241 ttattgctgt aggcagcggc ctgcgcggcg gtcatgttct tggaggtgga ggtaatcgcc
     9301 accagccctg ccacgccgat aatgatgcct aggattgtga ggaacgagcg caggcggcgt
     9361 ctcgtcaggt cgcgaaacgc cttgcgaagg gagataatca tgcttcttag atgtgctagg
     9421 gaggcagcct gatacgtttg agggcagccg atcctcgcag gtctcctgcc tatatatgat
     9481 tatacagttg cgcgagggtt attgcctaag cgccacccga ccattgagct acagagaagt
     9541 attgactcga tacaggctcg ctgatataat ctgcctaccc catgcttcta cattgcagca
     9601 ctgtcgtcgc gcattcatac ctgctagctc tatgatatgg gtttagttgg cattacttca
     9661 ctttgagcaa tgaggccgta cgaaacacga tttagggagg caattacaga tgttcaagat
     9721 gtcgagcagc cctggtgccc cgcgcaccca acccctacgc cgttccaccc ggctcatctc
     9781 catactatct ctgctcctgc tgctggcgtt cttgctgctg ttacgcgaga cctcgttgca
     9841 ggctgaggtg acgcatgcag ccggcctcgg gagcgttccg gcgcaggctg acaggttcgg
     9901 gcagaaggac gggcggaaag aggcaccacc cagcgtcgag caagcgggag gccgcaactc
     9961 acaggtgcca cagggtgcct gctcgcagac gcagatagac gaagggttcg atgacatcac
    10021 aaccctctca gcttcgggct ggtttactca gaacaacagc agtccacaag gcgtcacagg
    10081 gtggttccag ggcaaccccg aagcattccc cgcacattct ggtgccgcta actcttatgt
    10141 tggggcgaac ttcaacaaca ccaccggcag taatactatt agcaactggc tgctcagccc
    10201 tcaggtttct ctgcaaaacg gtgatatgtt cgtcttttgg acgaggacgt ctgaagacaa
    10261 cccgttgccc gaccggctgg aagtaagact tagcaccgca ggtgcgagca ccaacgtagg
    10321 cgacacttcc acaagcgttg gagactttac tgtattgctc cgctctatta accctaacct
    10381 ggaagtaggt ggatatcctg aggtctggac gcaattcgtt gtcactctga acgtcttctc
    10441 gggcaccgaa agcggcagaa tcgcctttcg ctattacgtg accgatggtg gccctgcggg
    10501 ggacaactcc aactacatcg gtattgacac agtggcctac tacactggtt gcagccctca
    10561 ggctactccc acggccacag cgtgcccggt ccagttcaac gacgtgccga gtagcagccc
    10621 cttctacgca aacgtgaggt gtatggcctg tcgaggcgtt atttcggggt acgggtgtgg
    10681 ggccgcggat gagccgtgcc ccggctccta cttcagacct ggtgtgaacg tcaccagggc
    10741 gcagatctcc aagatggtgg ctattgccgc cggccttgat gatgagccca caggcgctcg
    10801 caagttccag gacgtgccgg agggttcgac cttctaccaa tgggtacagc agttggcgaa
    10861 cagcggggct ataagtggct atgcctgtgg cggcgccaac cctgctaatg gtgaagccga
    10921 gccgtgtgta gcgcctggta acctggcgta cttccggcct aacaacaaca ccactcgcgg
    10981 gcaactcaca aagatcgtga gtacggcggc agcgctcaca ggagacccag gctcccagaa
    11041 gttcgcggat gtgcccccta atgggacatt ctttgagtgg gtgcagcggc tgtcgaatag
    11101 aggagtggtg ggtggctact cctgtggagg caccaacccc gccacaggag cgccagaacc
    11161 atgtgacgct gcatcacgcc catacttcag ggtgaacaac aacgtcacgc gtggtcagat
    11221 tgccaagatc gccgctgaga ctttcttccc caattgccag acccctgcca ggtcaataaa
    11281 gtagcagaag accgtctcac tcgcaaggac cgaatgaaga agagcctcgt gcctacgggc
    11341 agcagcgagg ctcttcttga ttgtaa
//
LOCUS       DASRWZ010000305         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_917205, whole genome shotgun sequence.
ACCESSION   DASRWZ010000305 DASRWZ010000000
VERSION     DASRWZ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917205"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..734
                     /locus_tag="VFH60_04780"
     CDS             384..734
                     /locus_tag="VFH60_04780"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HET6313128.1"
                     /translation="MQDKPRYTFHYTPEEYFTGLDPAADYLPPEFEEDGFIHCTDGAE
                     NVARVATAYFRYDERRFLVLYIDKTLVTSPIKYEDPGEIYPHIYGPLQREAIVRVLAA
                     WRDYDGFFLPMPDM"
     gene            complement(779..>1538)
                     /locus_tag="VFH60_04785"
     CDS             complement(779..>1538)
                     /locus_tag="VFH60_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313129.1"
                     /translation="FALLSGELYYNLHGFLLASPFVALALWPTRQVPTGPGADVGGRP
                     PLTPHAWLQTVTLLYVGLHAFIICVLSGLGPISRHEWGQRYLLPAYPALVVLSLLAVF
                     RIADAYRGRRWTLHRGTLVPACSALVLIGVLLSVRGYAATITERRQVVAWQALAGTLP
                     GREPLVTDVWWLPLNLAPVFYTRPIMLAEGDERLGEWARQMQAKGVTTFGLMSDSPEV
                     YSGRWLLSVPGLRAESPPAEEQGIWLQRYSLDNR"
BASE COUNT          285 a          487 c          472 g          294 t
ORIGIN      
        1 gtcagcatca tggtacgtag cgttcaacaa ccttcctgtt ccggcttctt tagattcttc
       61 gctgcgctca gaatgacagg ggccactttg gtcccgcacg agcggcactt tgcagattga
      121 ccctagtccc cgaagggaga ctttgtactg tgtagcccgc gttttcaatc gccgggcgtc
      181 ggctggcgac tatgtggctt ttacatacac cgccggatgt cggcgcggcg tttcagttgg
      241 cactcctgtt ataatcaagc accacaggca ttgcggcaag aggtagaggg ttgtttctcg
      301 cgccttagct gctcctcaga cctcggttgt atatcgggca ttccaaagca ctttgaattt
      361 tcgccctcca cacatgagga accatgcaag acaagccgcg ttacaccttc cactacacgc
      421 ccgaagagta cttcaccggc ctcgacccgg ctgccgacta cctgccgccg gagttcgagg
      481 aggacggctt catccactgc acggacggcg cggagaacgt cgccagggta gctacggctt
      541 acttccgcta cgacgagcgg cgcttcctcg tactgtacat tgacaagacg ttggtcacca
      601 gccccatcaa gtacgaggac cccggcgaga tttacccgca catctacggc ccgcttcagc
      661 gcgaggctat agtgcgcgtg ctcgctgcct ggcgcgacta cgacggcttc ttcctgccga
      721 tgccggatat gtagggctgt aggggagggc gaggtctacc gcgttcctgg gtgccggtct
      781 acctgttgtc cagggagtac cgttgcaacc atatgccctg ctcttccgcg ggcggggact
      841 cggcgcgcag gccgggcacg cttagcagcc accggccgga gtagacctcc gggctgtcgc
      901 tcatgaggcc gaaagtggtg actcccttcg cctgcatttg cctggcccat tcgcccaggc
      961 gctcgtcgcc ctccgccagc atgatgggcc tggtgtagaa gacaggggcg aggttcagag
     1021 ggagccacca tacgtcggtg acaagtggtt ctctgccggg gagggtaccg gccagcgcct
     1081 gccacgcgac cacctgcctg cgctccgtga tggtggctgc ataccctctt acgctcaaca
     1141 gcacgccaat cagcacgagc gcgctacatg ccggcaccag cgtgcctctg tggagcgtcc
     1201 agcgtctgcc tcggtaagcg tccgcgatgc ggaagacggc cagcagcgac agcaccacca
     1261 gggcggggta ggcgggcagt aaataacgtt ggccccactc gtgcctgctt atcggtccca
     1321 ggccgctcaa cacacagata atgaaggcgt gtagccccac gtacagcagc gtgacggttt
     1381 gcagccaggc atggggcgta agagggggtc gcccacccac gtccgcgcct ggacccgttg
     1441 gcacctggcg tgtgggccac agggccaggg ccacgaacgg gctggcaagc aggaagccgt
     1501 gcaggttgta gtacagctcg ccggaaagca gggcgaac
//
LOCUS       DASRWZ010000306         4317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_711308, whole genome shotgun sequence.
ACCESSION   DASRWZ010000306 DASRWZ010000000
VERSION     DASRWZ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711308"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1922
                     /locus_tag="VFH60_04790"
     CDS             99..1922
                     /locus_tag="VFH60_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET6313130.1"
                     /translation="MREHSKGRLYKVLNILVLLLLVAGSAACGGSYGNAPSATTTSGL
                     RVAPSDPIAAGNKATGGVAAFEATVTTGTAALADIVTATGGGGSTEAPISGARPAEST
                     TGEDGTLRTGSMPPPNYQSSLTAGQVDDNARFREYLEYLREHRSEYATQMDVSQRLFL
                     RVVDGEQRPVAGARVQLYDGQRLLYDGQTVSDGRVLFFPSLVGAGQVHALRAVVSRGN
                     SAVEANVNVAGPEQTISMPDLKDNSGPVALDLVFLLDATGSMGDEIAEIKATVGSIAQ
                     RIEQLPGSSAPRFGLVAFRDLGDDYVTRRWDFTSDIGRFAVNLSNVSAGGGGDVPESV
                     NAGLHDAINLPGWADNSTGQHLRMIVLVGDAPPHLDYPNDYAYPDLLQQAVAKGIKIF
                     PIGASNLDSGGEYIFRQFAQFTQGQFVFLTYANGVSGAPGASTTHDVPSDFTVQNLDS
                     LVVRLVQTEVANQTGQQPQGSRPVMVPVAAMPGPVEQPATLLASLQGTLQHAAGEVFS
                     WTSLLWLALLAGMLIVAQRFPQRVRVAPAAATPNEASEAETVHGDIVAPDWDELEVPH
                     PTVSTTHPPYIDYAAPQLTVPLPNLEAALRDYKELSAIQDR"
     gene            complement(2453..4132)
                     /locus_tag="VFH60_04795"
     CDS             complement(2453..4132)
                     /locus_tag="VFH60_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET6313131.1"
                     /translation="MEGFNGESFALILALIGVVIMIAALISGLIERSNLPQVAVFLAL
                     GAVLGPPGLGIVNVTLDSPVLRVVATLSLTLVLFTDALSLNLQDLRKHLGLTVLVLGP
                     GTLLSAVLIAGLGWWLLGLPLAAAVIVGAALASTDPVLLRGFLRRRDIPSTTRQALRL
                     ESGLNDVVLLPIVLVAMTFLGLGNGSGGAAESPNWARLALDLLLLGPGAGVLIGLLAV
                     ATLDLVRRRIGIRRDYESLYSLGVAFAAFAAAEAVHGSGFLAAFAAGLTIAALDVELC
                     DCFLEYGETTAEMALLFTFVLFGSSLIWTAFNVWSLAAVLFAVLVLLVRPLAFSIALL
                     RTRLNRYDRFLISWFGPRGLSTLLLVLLPVFAGVEGGAQMFAICSLVVLLSVVVHGGT
                     LMLVGKGKRGSATPQEGSTLAPATAALSSGVGLGKAAPLPLISSPPPLESARGDGALD
                     GRAWVGEGAGHGLSGGTSASWEREPEHEGSELPEHDRIDVERLRKLQQAGERVGVVDS
                     RTDRTYDSSDLDATGAVRVVPGSGGTAPGSIIDQAMQLHVPRDAWIVVFCA"
BASE COUNT          841 a         1474 c         1358 g          644 t
ORIGIN      
        1 accaatttgg gccgaatggg aacattcctc aggcgggtga cgttcatcct ctactcttgg
       61 caacttgtct acggtttgta gcctgaggag gccccaaagt gagagagcat agcaaagggc
      121 gattgtacaa ggtcctcaac atcctggtgt tactgcttct cgtcgctggc agcgcagcct
      181 gcggaggctc gtacggtaac gcccccagcg caaccacaac gagcggcctg agggtagcac
      241 cgtccgaccc aattgcggct ggcaataagg ctacaggagg cgtggcggcg tttgaagcca
      301 cggttaccac gggcacagcc gccctcgcgg acatcgtaac tgcgacgggg ggcggcggtt
      361 cgacggaggc gcccatatcc ggggccaggc ctgccgagtc tacaacaggc gaggatggta
      421 cgttgcgcac gggcagcatg cccccaccga actaccagtc gtcgctgaca gccgggcagg
      481 tggatgacaa cgccaggttc cgggagtacc ttgagtacct gcgcgagcac aggtctgaat
      541 acgctactca gatggacgtg tcccagcggc ttttcctcag ggtcgtggac ggcgagcagc
      601 ggccggtggc cggggccagg gtgcagcttt acgacggcca gcgcctgcta tacgacgggc
      661 agacggtgag cgacggccgg gtgctcttct tcccgtcgct ggtgggtgcg ggccaggtgc
      721 atgccctgcg cgcggtggtc agccggggca actcggcggt ggaagccaac gtcaacgtcg
      781 ccgggccgga gcagaccatc agcatgcccg accttaagga caactccggc cccgtggccc
      841 tcgacctggt attcctgctc gacgccaccg gcagcatggg ggacgagata gctgaaatca
      901 aggccacggt cggcagcatc gcacagcgca tagagcaact gccgggcagc agcgcgcctc
      961 gcttcgggct ggtggctttc agagacctgg gcgacgacta cgtcacccgc cgctgggact
     1021 tcaccagcga cattggccgg ttcgcggtga acctgtccaa cgtatcggcg ggaggtggcg
     1081 gcgacgtacc ggagtcggtc aacgcgggcc tgcacgacgc tatcaacctg cccggctggg
     1141 ccgacaacag cacggggcag cacctgcgca tgatagtgct cgtcggcgac gccccgcctc
     1201 atctcgacta cccgaacgac tacgcctacc ccgacctctt acagcaggcg gtcgcgaagg
     1261 gtataaagat tttcccgata ggcgcgagca acctcgatag cggcggcgag tacatcttcc
     1321 gccagttcgc ccagttcacg caggggcagt tcgtcttcct gacctacgcc aatggagtgt
     1381 cgggcgcgcc gggggcatcc accacgcacg acgtgccctc ggacttcaca gttcagaacc
     1441 tcgacagctt ggtggtgcgg ctggtacaga ctgaggtcgc caaccagacg gggcagcagc
     1501 cacaaggcag ccggccagtc atggtgccgg tcgccgccat gccggggcct gtggaacagc
     1561 cggccacgct gctggcttcc ttgcagggca cgctccagca cgccgccggc gaggtcttta
     1621 gctggactag cctcctctgg ctggcattgc ttgcgggcat gctcatagta gcccagcgct
     1681 tcccccagcg ggtcagggtc gcaccggccg cggccacccc aaacgaggct agcgaggctg
     1741 agaccgtcca cggcgacatc gtggcaccgg actgggacga gctggaggtg ccgcacccca
     1801 ccgtctcaac aacccacccg ccctacatcg actacgccgc cccgcagcta acggtgcccc
     1861 tccccaacct ggaggcggcc ctgcgcgact acaaggagtt atccgctatc caggaccgct
     1921 aagctatagg ggaaggcccc gatgcggcct cgtcgcggtg gataactcag cgtggcaagg
     1981 tgcggctatg ctaccctgcg agcaggtcca ctcgccgtgc aacgtttggc tatccctggc
     2041 ctgcggcgag tggacctttt tcgttgtccg cgtacggcct ggccttatga cgtgacggca
     2101 aggcggctct ttagtggccc tgcgatagcc ctgccacggt tgccctgtgg ctaaagccac
     2161 gggctcacaa gaggcaaagt cccctacggg gactgaggag catcatgtag tctcctacgg
     2221 agacttcgtc tgtggtcagc ccgccaattc attggcaggg cacggtggca gggcgtccat
     2281 acagggcaca cccacaggac tatcccaggg cgtctaatca cgcggcggcc ctggcctgga
     2341 ccggcagacc agcagcctgc caggcttccc acccgccggt aagggcacgc gccctgcccc
     2401 agccgccgcg ctgtaactct cgcaccacac gggcactcgt cgcttcgttc cgtcaggcac
     2461 aaaataccac tatccaggcg tcccgcggaa cgtgcaactg catcgcctgg tcgataatgc
     2521 tccccggcgc ggtgcctccc gaccccggca cgacccgcac ggcccctgtc gcgtccaggt
     2581 cgctgctgtc gtaggtgcgg tcggtgcgcg agtcgaccac accgacccgc tctccggcct
     2641 gttgcagctt tcgcagccgc tcgacatcga tgcggtcatg ctccggcagt tcagaccctt
     2701 cgtgttccgg ttcgcgctcc cagcttgcgg aggtaccgcc ggaaagcccg tgtccggccc
     2761 cctcccctac ccaggcgcgc ccatccagcg caccgtcccc cctcgcactc tccaacgggg
     2821 gcggactcga gatgaggggt agcggggctg ccttgcccag gccgacgccg gaactaaggg
     2881 ctgcggtcgc aggagccagg gtggacccct cttgcggagt ggcgctgcca cgcttaccct
     2941 tgcccaccag catcagggtg ccgccatgca ccaccaccga cagcagcacc accagcgaac
     3001 atatggcgaa catctgcgcg ccaccctcga cccctgcgaa caccggcaac aacaccagca
     3061 gcaaggtgct caggccgcgc ggcccaaacc acgatatgag gaacctgtcg tacctgttga
     3121 gccgggtgcg cagcagggcg atagagaagg caagagggcg cacgaggagc accagcaccg
     3181 cgaagaggac agccgccagg ctccacacgt tgaaggcggt ccagatgagg gagctaccga
     3241 acagcacgaa ggtgaagagc agggccatct cggcggtcgt ctcgccgtat tcgaggaagc
     3301 agtcgcacaa ctccacgtcc aacgcagcta tcgtgaggcc ggccgcgaac gccgccagga
     3361 agccgctgcc gtgcaccgcc tcggccgccg cgaaggccgc aaacgccacg cccagcgagt
     3421 agagcgactc gtagtcgcgg cggatgccta tcctgcggcg cacgaggtcg agggtagcca
     3481 cggcaagcaa gccaatcagc acgccagcgc ccggccccag cagcaggagg tcgagggcca
     3541 gcctcgccca gttggggctt tcagccgcgc cgcctgagcc gttccccagt cccaggaagg
     3601 tcatcgcgac caggacgatg ggcaggagca ccacgtcgtt gaggccgctt tcgaggcgca
     3661 acgcctggcg ggtcgtagag gggatgtcgc ggcggcgcag aaagccccgc aacagcacgg
     3721 ggtcagtgga ggcgagggcc gcgcccacta tcacggccgc ggcgagcggc aggcccagca
     3781 gccaccagcc cagcccggca atgagcacag ccgaaagcag agtacccggc cccagcacca
     3841 gcaccgtcag cccgaggtgc ttgcgcaggt cctgtaggtt gaggctgagc gcgtcggtga
     3901 agagcaccag cgtcaggctg agcgtcgcca ccacccgcag cacgggcgag tcgagcgtga
     3961 cgttgacgat gccaagcccc ggcgggccga gcaccgcccc cagcgccagg aacacggcca
     4021 cctgcggcag gttgctgcgc tcgatcaacc ccgatatgag cgccgcgatc ataatgacca
     4081 cgccgataag cgccaggatt agcgcaaacg actccccgtt gaacccttcc atctaactcc
     4141 aacctccagc ttccactcta cacgcaatca ccaccgcttt caaccaccca aaccggtagt
     4201 atgtacaggg cgatgcggtg aagagacgaa aacgcaaaga cacaaaggac gcaaaggacg
     4261 cgcagaaatt aagattgttg ttctaagcaa aaggaccaga gtacgtcatt tcgtacc
//
LOCUS       DASRWZ010000307         3620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1328062, whole genome shotgun sequence.
ACCESSION   DASRWZ010000307 DASRWZ010000000
VERSION     DASRWZ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328062"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFH60_04800"
     CDS             complement(<1..556)
                     /locus_tag="VFH60_04800"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HET6313132.1"
                     /translation="MRVISGTAKGRTLKGPATRGTRPMTDRLKTSLFDTLVPYGVGGA
                     RVLDLYAGSGSLGIEMLSRGAEWADFVEHNAGVVRIIEDNLASTGLAGRARVYKMDVG
                     AFLTMRAKAHEPARGVVDTAADYDIILLDPPYADPAINNMLETIATGPLLAEDGILVI
                     GHATQVPLADEYGSGRLKRARHRVM"
     gene            complement(730..3294)
                     /gene="recG"
                     /locus_tag="VFH60_04805"
     CDS             complement(730..3294)
                     /gene="recG"
                     /locus_tag="VFH60_04805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET6313133.1"
                     /translation="MPIPTTVFRNILRLEEHKGYADASVQGGLEAFARRWSEQIVSAE
                     RGAKAMAEAIAGAFGGYAAVEVGERERAVRRANELLALFDEGVRVVPGMNGKHAGGKN
                     GASGNGHASHGIEGFTGFDDTDPRAKAPISSFRSGIAPPPLVEAPAAPARSARAARRS
                     ATLDSPVTVLRGVQEATARLLKRLGVYTVRDALLFFPFRYDDFSEMKSIAELRPDVNQ
                     TVVGTIWSVEVRPTRNGRPMVTATVSDDSGIILVTWFNQDYIARQLHQGDAIVISGKP
                     AEFNGRLQFSSPEWEPYDSELLHTGRIVPVYRSTEGLYRRTLRRIMHDAVERFADQLT
                     DYLPEAMKRDAQLLDLVTAIRQIHFPESKLDAAKATKRLAFDEFLMIQLGMLQRKRAW
                     QESQKGLPLKIEGDDLQHFYSSLPFALTDAQRRVIGEITADISGTVPMTRLLQGDVGS
                     GKTVVAAAALYLAVRNGAQAVLMAPTEILSEQHYKSLSRMMAALERDGQPAPTVRLLK
                     GSTTRKEKNEIYAATASGEIDILVGTHAVIQENVAFKRLAMIVVDEQHRFGVMQRDAL
                     RQKGIEAGLMPHTLVMTATPIPRSLALTIYGDLEVSVIDEMPPGRRLIKTRWVDATRR
                     NQAYEFIEKKVAEGRQAFVICPLVEESDKIEAKAAVAEYERLQSEVFTGRKLALLHGR
                     MKPKEKEEIMASFNRGDADILVSTSVVEVGIDVPNATVMLIEGADRFGLSQLHQFRGR
                     VGRGEHQSYCVLLAEDAQGVAFERMQIIQETHDGFRLAEEDMKIRGPGQFFGTRQSGM
                     PDLRMAKLSDMATLALAREQAKALFTRDPDLSRPEHAPLKDVLEAFWSRAETDANQ"
BASE COUNT          621 a         1190 c         1142 g          667 t
ORIGIN      
        1 ccataaccct gtgcctggcg cgcttgaggc gtccgctacc gtactcgtcg gccaacggga
       61 cctgcgtggc gtgccctatc accagtatgc catcctccgc cagcaacggc cccgtagcga
      121 tggtttccaa catgttgttt atcgcagggt cggcgtacgg agggtcgagg agtattatat
      181 cataatcggc ggcagtgtct accacgccac gcgcaggctc gtgcgctttg gcacgcatgg
      241 tgaggaacgc gcccacgtcc atcttgtaga ctcgcgcccg gcccgccagc cccgtgctag
      301 ccaggttgtc ctcgattatg cgcaccaccc ccgcgttgtg ctccacgaaa tcggcccact
      361 cagcgccccg cgacagcatc tctatcccca gcgaaccact cccggcgtac aggtcgagca
      421 cccgcgcccc gccaactccg taaggcacga gcgtgtcaaa cagggaggtc ttgagcctgt
      481 cggtcatggg ccgcgtgccc ctggtggcag ggcctttgag cgtgcggcct ttggctgtgc
      541 cggagataac tctcatgcgt tgtaacccgt aataccaacc atttagaaaa gaattataag
      601 agaaaggggg agaactgggg gacgccccca gccccccgtc aggggagcac ccctgcaccc
      661 ccttgtgcgg gagagcactg aaggacaccc tgacgccccc acgggggagt acccctgcac
      721 cccgctcgac tactggttgg cgtccgtctc ggcgcggctc cagaaggctt ccagtacatc
      781 cttcaggggg gcgtgttccg gccgggagag gtcggggtcg cgggtgaaga gggctttggc
      841 ctgctcgcgg gcgagggcaa gcgtcgccat gtcggagagc ttcgccatgc gcaggtcggg
      901 catgccggac tgacgggtgc cgaagaactg gccgggtccg cgtatcttca tgtcttcttc
      961 ggccaggcgg aagccgtcgt gcgtctcctg tatgatctgc atgcgctcga aagccacccc
     1021 ctgagcgtcc tcggccagca gcacgcagta cgactggtgc tcccccctgc ccacacggcc
     1081 ccgaaactgg tgcaactgcg agaggccgaa gcggtccgcc ccctctatca gcatgaccgt
     1141 ggcgttaggc acgtcgatac ccacttccac cacgctggtg gacaccagga tgtcggcatc
     1201 tccccggttg aaggaggcca tgatctcctc cttctccttc ggcttcatcc gcccgtgcag
     1261 gagcgcgagc ttgcggccgg taaacacttc cgactgcaac cgctcgtact cggccacggc
     1321 ggccttggcc tctatcttgt ccgactcctc cacgagcggg cagatgacga acgcctgcct
     1381 gccctcggcc accttcttct cgatgaactc gtaagcctgg ttgcggcgcg tggcgtccac
     1441 ccagcgcgtc ttgatgaggc gtctgccggg cggcatctca tcgatcaccg acacctcaag
     1501 gtcgccgtag atggtgaggg ccaacgagcg ggggatgggg gtggcggtca tcaccagcgt
     1561 gtgcggcatc agccccgcct cgatgccctt ctggcgcagg gcgtcgcgct gcatcacgcc
     1621 gaagcggtgc tgttcgtcca ccacgatcat cgccagccgc ttgaacgcca cgttctcctg
     1681 tatcaccgcg tgagtgccca ccaggatgtc aatctcaccg gaggccgtgg cggcgtagat
     1741 ttcgttcttc tccttgcgcg tggtgctgcc cttcaacagc cgcaccgtgg gggccggttg
     1801 cccatcgcgc tccagggccg ccatcatgcg cgacaggctc ttgtagtgct gctccgacag
     1861 gatttcggtg ggggccatga gcacggcctg cgccccgttg cgcaccgcca ggtagagggc
     1921 cgcggccgcc accaccgttt tgccggagcc tacgtcccct tgcagcaggc gcgtcatggg
     1981 caccgtaccc gaaatatcgg cggtaatctc gccaatcacc cgccgctggg cgtctgttag
     2041 cgcgaagggg agggaggaat agaagtgttg caggtcgtct ccctcaatct tcagaggcaa
     2101 acccttctga gactcctgcc acgcccgttt gcgctgtagc atgccaagct ggatcatgag
     2161 gaactcatcg aaggcaaggc gcttggtggc tttggcggcg tccagtttgc tttcggggaa
     2221 gtgtatctgg cggatagcgg tcacgaggtc gagcagttgg gcgtcgcgct tcatggcttc
     2281 cggcaggtaa tcggttagct ggtccgcgaa acgctccacg gcgtcgtgca tgatgcgccg
     2341 cagcgtgcgc ctgtacagcc cttcggtcga gcggtacacc ggcacgatgc gccctgtgtg
     2401 cagcagctcc gaatcgtaag gctcccactc aggcgagctg aactgcaagc ggccgttgaa
     2461 ctcagcgggc ttgcccgaaa taacgatggc gtctccctgg tgaagctggc gggcgatgta
     2521 gtcctggttg aaccacgtca ccaggatgat gccgctgtcg tccgacaccg tagccgtgac
     2581 catcggccgc ccgttgcgcg tgggccgcac ctccaccgac cagatagtgc ccaccaccgt
     2641 ctggttcacg tcgggccgaa gctccgctat cgacttcatc tccgaaaagt cgtcgtaccg
     2701 aaacgggaag aacagcaggg catcgcgcac cgtatagacg ccaagccgct tcagcagccg
     2761 cgcggtggcc tcctgcacgc cgcgcagcac agtgacgggc gagtcgaggg tggcgctacg
     2821 gcgggccgca cgcgcgctcc tggccggggc cgcgggggct tccaccaggg gcgggggcgc
     2881 gataccgctg cggaacgagg agatgggggc cttggcgcgc gggtcggtgt cgtcaaagcc
     2941 ggtgaagccc tctatgccgt gcgaagcgtg gccgttgccg gaagccccgt tcttgccgcc
     3001 cgcgtgcttg ccattcatgc cgggcaccac gcgcacgcct tcatcgaaca gagctagcaa
     3061 ctcgttagcc ctgcgaacgg cccgctcccg ttccccgacc tcgaccgccg cgtacccgcc
     3121 gaacgccccc gctatagcct cggccatcgc tttagccccg cgttcggcac tcactatctg
     3181 ctcggaccac cgccgcgcga aagcctccaa cccgccctgc accgaggcgt cggcgtatcc
     3241 cttatgctcc tccaaccgga ggatgttgcg aaatacggtg gtaggtatcg gcatggggct
     3301 gcgggtgact ggtggcacgt gacgcgtaac gcgtaacacg taacacgtaa cacgtaagtc
     3361 gccattgagc ttgtggtgag gattagcgcg tctgttctta ttgtaccgca gcgggcgggg
     3421 gaagggaagc gaggtggaaa agggttttgg ggttgaaggc ggatgccctg tgggtaatag
     3481 ttacagcgag gagatacgac ccctcgcggg ttaattcaca ggctacaaca tggtcgccag
     3541 ccggcgcccg tcgattgaaa tcgagggcta cacctggcaa agtctgccgc agggcagact
     3601 ggtgcatctt gagagtgcct
//
LOCUS       DASRWZ010000308         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_300521, whole genome shotgun sequence.
ACCESSION   DASRWZ010000308 DASRWZ010000000
VERSION     DASRWZ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300521"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..987)
                     /locus_tag="VFH60_04810"
     CDS             complement(82..987)
                     /locus_tag="VFH60_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET6313134.1"
                     /translation="MLDLLRDLIFDYTLRNVALGSALLGIVSGVLGTFAVLRRQGLLG
                     DALAHAALPGICVAFMLTGSKAPLVLLLGAAVAGWLGTLLLLYVVRETRISEDAALGV
                     VLSVFFGVGIMLLTLISRSNNANQAGLDKFLFGQAATLVWDNVLTMMVLGAVALFVVG
                     LLFKEFKLLTFDTDFAASLGFPTNRLSILLTSLIVIAVVIGLQTVGVVLMAAMLIGPA
                     VAARQWTNRLGVMVGVSALIGALSGVIGALISVSDVGLPTGPMIILSLTAIVALSLLF
                     APERGIVWDRLREIRNRRLMREDAA"
     gene            complement(996..1775)
                     /locus_tag="VFH60_04815"
     CDS             complement(996..1775)
                     /locus_tag="VFH60_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET6313135.1"
                     /translation="MSASAAEIPVAINDLTVAYREKPVLRDIDLVIPPGKLAVIMGPN
                     GAGKSTLIKAVLDLVPAAAGQSLIFGRPYREQRKLVGYVPQRGSVDWDFPTTALDVVM
                     MGTYGKLGWLKRPGKTERDWAMHCLEQIGMADYANRQISQLSGGQQQRVFLARALAQD
                     ARLYFMDEPFVGVDATTERAIVTLLQELQAQGKTVVCVHHDLESATDYFDWLVLLNVR
                     LIATGPLATTFTEENLVKTYGGRMHALRVHTAAQQAQQVAG"
BASE COUNT          364 a          633 c          659 g          368 t
ORIGIN      
        1 ccagcatgtc cattactctt gccagttccg ctcccggaaa gacctggctt gctttagaca
       61 ttacgcggcc ccgtgtcacc ttcaagccgc gtcctccctc atgagccgcc tgttgcgtat
      121 ctcgcgcagg cggtcccaga ctatgccgcg ttcgggcgcg aacaggaggg agagggcgac
      181 gattgcggtg aggctgagga tgatcatggg gccggtgggt aagcccacgt cgctgacgct
      241 gatgagggcg ccgatcacgc cggagagggc gccgattaga gcgctcacac ccaccatgac
      301 gcccaggcgg ttggtccact ggcgggcggc gacggcgggg cctatgagca tggccgccat
      361 cagcacaacg cccaccgttt gcaggccgat taccaccgct atcacgatga gggaggtgag
      421 caggatgctc aggcggttgg tggggaagcc gaggctggcg gcgaaatcgg tgtcgaaggt
      481 gagcagcttg aactccttga agagcagccc caccacgaag agggccaccg cgcccagcac
      541 catcatcgtc agcacgttgt cccacacgag cgtggccgcc tggccgaaca ggaacttgtc
      601 aagccccgcc tggttagcgt tgttgctgcg cgagatgagg gtgaggagca tgatgcccac
      661 cccgaagaag acgcttagca ccactcctaa cgccgcatct tcgctgatgc gggtctcgcg
      721 caccacgtaa aggagcagca atgtgcccag ccagcccgcc accgccgcgc ccaacaacag
      781 caccagcggc gccttggagc cggttagcat gaaggccacg cagatgccgg gcagggcggc
      841 gtgggccagg gcgtcaccga gcagcccctg ccgccggagc accgcgaagg tgcctagcac
      901 gccgctgacg atgccaagca gcgcggagcc gagggccacg ttgcgcagcg tgtagtcgaa
      961 tatcaggtcg cgtagcaggt cgagcatata cgttcctacc cggcgacctg ttgggcctgc
     1021 tgggcggcgg tgtgcacccg cagcgcgtgc atgcgccccc cgtaggtctt gacgaggttc
     1081 tcctcggtga atgtggtggc gagcgggcct gtcgcgatga ggcgcacgtt gagcagcacg
     1141 agccagtcga agtagtcggt ggcggactcc aggtcgtggt gcacgcacac caccgtcttg
     1201 ccctgcgcct gcaattcctg taacagggtg acgatagccc gctcggtggt ggcgtccacg
     1261 cctacgaacg gctcgtccat gaagtagagc ctggcgtcct gggcgagggc gcgggcgagg
     1321 aagacgcgct gctgctgccc gccggaaagc tggctaattt ggcggttcgc gtagtcagcc
     1381 atgcctatct gttcgaggca gtgcatcgcc cagtcgcgct cggtcttgcc gggccgcttg
     1441 agccacccca gcttgccgta ggtgcccatc atcaccacgt ctaacgccgt ggtggggaag
     1501 tcccagtcca cgctgccgcg ctggggcacg tagcccacga gcttgcgctg ttcgcggtac
     1561 ggcctgccga agatgaggga ctgtccagcc gccgcgggca ccaggtcgag caccgccttg
     1621 atgagcgtgg acttgcccgc cccgttcggc cccataatca ccgccagctt gccgggcggt
     1681 atcaccaggt ctatatcccg cagcaccggc ttctcgcggt aagccaccgt gaggtcgttg
     1741 atggcaacgg gtatctccgc cgccgaggcg gacattgcgt gctctgttat gtttgttgct
     1801 tgcatagttt gccccatatt gaccatgtat gtctccttgt gtgggggtga cgattgctgc
     1861 ggattgtgag ggcctgatgc atgagtgtat gatagttact attgcgggac cgagtccccc
     1921 cattgtcatt ctgagcgtag cgaagaatct cgtccctcag cacccaaatt ccacctttct
     1981 cgaggccaat aggcagtcat ggtgctattg aaaagggaag catc
//
LOCUS       DASRWZ010000309         1958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_231369, whole genome shotgun sequence.
ACCESSION   DASRWZ010000309 DASRWZ010000000
VERSION     DASRWZ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231369"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1363
                     /locus_tag="VFH60_04820"
     CDS             35..1363
                     /locus_tag="VFH60_04820"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET6313136.1"
                     /translation="MAAPLADSHPVESIDRSRLLNLLALAMITVVFAWGAWSWVQFFR
                     SPQGGAGSHTGLFTQTDYPAVVIASRIVSSGDAHQLYDLNWQREGQLRLIREGYLHQP
                     LDAPLLYPYPYAPFVAVLNAPFAAQSPLLAMAIWDLINIVCMALGLWVLLSALPLARL
                     TRCLLLLGGLTCFPFIVNLEQGQSSGVIMFGMAVGIARLRRGDDLGGGVALGLLLLKL
                     QWLPFLVLVLLFKGRWRALLGIAATGLALLLLSLLTISTGWIPGFLDMLQRAQRFDPA
                     LALTPWASHSLTGQLAALLFGSAANVNAAGNEVIRNVTLAATLLAAGLVVVLWRGQWR
                     PGSRNWDGAMSLTILATAFTNLQLNTHDLCLLALPAALGVAYFRTSAGQEPLGRAWYG
                     LVVITYLGTSLAFPLLFAAPLRLMPLLLGLMMVLVTVSLLRSHVPASTAT"
     gene            1435..1902
                     /gene="ndk"
                     /locus_tag="VFH60_04825"
     CDS             1435..1902
                     /gene="ndk"
                     /locus_tag="VFH60_04825"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET6313137.1"
                     /translation="MERTLVLLKPDAVQRGLLGEIIGRFERKGLKLAGMKMMQLDDTL
                     LDEHYSHLNHLPFFGDIKQFMMRTPVIATCWEGVDCVQAVRAMCGKTLARDADPGTIR
                     GDYAMSIQANLVHASDSIETAQAEVPRFFNDNELFDYDWLLTPYIYTAKERGE"
BASE COUNT          342 a          669 c          572 g          375 t
ORIGIN      
        1 cccgctacag accggctgag tgggagatgt atttgtggct gcacctttgg ctgactcgca
       61 ccctgtagag agcatagacc gtagcaggtt gctcaacttg ctggcgcttg ccatgataac
      121 ggtcgtgttt gcctggggtg cgtggagctg ggtgcagttc ttccggtcgc cacagggtgg
      181 ggcgggcagc cacaccggcc tcttcaccca gaccgactac ccggccgtgg ttattgcctc
      241 ccgcatagta agctccggcg atgcccacca actctacgac ctgaactggc aacgtgaggg
      301 gcaactgcga ctcatccgcg aaggctacct ccaccagccg ctcgacgccc ccctgctcta
      361 cccctaccct tacgccccat tcgtagcggt gctcaacgca cccttcgccg cgcagtcgcc
      421 actgctggcg atggctatct gggacctcat caacatcgtc tgcatggcgt tgggtttgtg
      481 ggtgctgctg tctgccctgc cccttgcgag gctgacgcgc tgcctgctgc tcctgggcgg
      541 cctcacctgc ttcccgttca tcgtgaacct ggaacagggg caaagctccg gcgtcatcat
      601 gttcggcatg gcggtgggta tagcccggct gcggcgtggc gacgacctgg ggggcggggt
      661 cgcgctgggc ctgctgctgc tcaagctaca gtggctcccc ttcctcgtgc tcgtgctgct
      721 gttcaagggc aggtggcggg cgctgctggg tatagcggcc acggggcttg cgctcctcct
      781 gctgtcgctc ctcacgataa gcaccggctg gatacccggc ttcttagaca tgctgcaacg
      841 ggcgcaaagg ttcgatcccg ccctggccct tactccctgg gcgagccaca gcctcacggg
      901 gcaactcgcc gcgctcctct tcggcagcgc cgcgaacgta aacgcggcgg gcaacgaggt
      961 aatccgcaac gttaccctgg cagccacact cctcgcggcg gggctggtcg tcgtgctctg
     1021 gcggggccaa tggaggcccg gcagccgcaa ttgggacggg gccatgtccc tcaccatcct
     1081 tgccacggcc ttcaccaacc tgcaactcaa cacgcacgac ctgtgcctgc tcgcgctccc
     1141 cgcggccctc ggtgtggcct acttccggac aagcgccgga caggagccgc tcggcagagc
     1201 gtggtacggc ctcgtcgtca taacttacct tgggacctcg ctggccttcc ccctgctgtt
     1261 cgccgcgccc ctccggctga tgcccctcct tcttgggctg atgatggtcc tggtgactgt
     1321 gtcgttgcta cgttctcacg ttccggcaag caccgccacc tgacccgcta ccagtccata
     1381 tgttataatc cctgccgact acgtcacaaa actagctacc caggagaata accgatggag
     1441 agaacactgg tgctgctcaa gccggacgcg gtgcagcgcg gactgttggg agagatcatt
     1501 ggccggttcg agcgcaaggg cctcaagctg gccggtatga agatgatgca gctcgacgat
     1561 accctgctcg acgagcacta ttcgcacctg aaccacctgc ccttcttcgg cgacatcaag
     1621 cagtttatga tgcgcacccc cgtgatcgct acctgctggg agggcgtcga ttgcgtgcag
     1681 gccgtccgcg ccatgtgcgg caagaccctc gcccgcgatg ccgaccccgg cacgataaga
     1741 ggcgactacg ccatgagcat ccaggccaac ctcgtgcacg cctccgactc cattgagaca
     1801 gcccaggccg aggtcccccg cttcttcaac gacaacgaac tcttcgacta cgactggctc
     1861 ctcaccccct acatctacac agccaaagaa cgcggcgaat agttggcgcg taagaataga
     1921 aaccgcaaag gcgcaaagag cgcaaaggac acggaaat
//
LOCUS       DASRWZ010000310         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_848848, whole genome shotgun sequence.
ACCESSION   DASRWZ010000310 DASRWZ010000000
VERSION     DASRWZ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848848"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VFH60_04830"
     CDS             complement(<1..835)
                     /locus_tag="VFH60_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313138.1"
                     /translation="MPLLVAPDAPSAVPQVNFMVSPLYDMFVSLSTMVHPGERHDAWA
                     SEIKRDLSPQMREEADYFYTLFENRLVELAVDYPDHGDVEGFFRYLENMAAEDFIFYA
                     TGRVMQPSEIASMSSHPLRLLAALLKLYGGEHRIHDQGWRQALQLLATEADTLRTRLL
                     RLLRTYWQQSYRHQIARLRPAWEESVRERTQSLGPDRLTAVQERFLANRTLPREFPEG
                     YPLEKVLLVPSLFLSMSSMILYGYGQLIIIYDALTSDEHRNAMAHVQRRIVAVARALE
                     DP"
     gene            complement(1131..1520)
                     /locus_tag="VFH60_04835"
     CDS             complement(1131..1520)
                     /locus_tag="VFH60_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008386959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET6313139.1"
                     /translation="MERKNYSTGAPWEPVVGYSRAVQVGPYIHVAGTTATDAEGNVVG
                     AGDAYAQTIQTLRNIERALQMAGAGMADVVRTRMFVTDISRWEEVVRADAEFFRNIRP
                     AATMVEVSRRISPDMLVEIEADAYVQP"
     gene            complement(1615..>2981)
                     /locus_tag="VFH60_04840"
     CDS             complement(1615..>2981)
                     /locus_tag="VFH60_04840"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HET6313140.1"
                     /translation="SASSYAAPSQSPAGAQSALPASLPFADSAFEAVWMRNDQLVAAK
                     SVARSWTWGPAPMAAGLEAYEEAPDGTRLRLVQYFDKARMEINNPKGTPTANGFVTNG
                     LLTVELISGLMQVGNSKFVTGKPAGINLASDPDDGNAPMYASFGSVSNTSAGEKRQPD
                     KTKGGYASQRISRTGDVTDDASKTKLAEARIVYYDKATGHNIPSVFWDFLNSKAQVRQ
                     GIGTASKPFLDPWVFAMGLPISDAYWANVKIGGKSQEVLIQAFERRVLTYAPDQPAGW
                     KVQMGNIGQHYFEWRYGPDGKGPEKLPAAKPSLPIYLSIPTMGVVSKVEYVGVDKDNN
                     MDIPKEAMNVGWFKPGTVPGNPGNAVMDGHLNWYGIPEAVFFHLDKLKAGDRVYVRDD
                     RGRDRAFVVTKQQTCVWNNCPLMDVFGPTKQTRLNLITCQGAFNRATQNYEKRLVVFT
                     EMVP"
BASE COUNT          534 a          893 c          984 g          570 t
ORIGIN      
        1 tggggtcttc gagggcgcgg gccacggcca cgatgcggcg ctggacgtgg gccatggcgt
       61 tgcggtgctc gtcggaggtg agcgcgtcgt agatgatgat tagctgcccg tagccgtaca
      121 ggatcatgga ggacatgctc aggaagaggg acggcacgag cagcaccttc tccagcgggt
      181 agccctccgg gaactcgcgg ggcagggtgc ggttggcgag gaaacgctcc tgcacggcgg
      241 taaggcggtc ggggcctagc gactgggtgc gctccctcac gctctcctcc cacgcggggc
      301 gcaggcgggc gatctggtgg cggtagctct gctgccagta ggtgcgcagg agcctgagca
      361 ggcgggtgcg cagggtgtcg gcctcggtgg cgagcagttg gagggcctgt cgccagccct
      421 ggtcgtggat gcggtgctcg cccccgtaca gcttgaggag cgcggccagc agtctcaacg
      481 ggtgggacga catgctggcg atttccgagg gctgcatgac gcggcccgtg gcgtaaaaga
      541 tgaagtcctc ggcggccatg ttctccaggt agcgaaagaa gccttccacg tcgccgtggt
      601 cggggtaatc gacggcaagc tccaccaggc ggttctcgaa gagggtgtag aagtagtcgg
      661 cctcctcgcg catctggggc gagaggtcgc gcttgatctc cgaggcccag gcgtcgtgcc
      721 gctcgccggg gtgcaccatc gtgctgaggg acacaaacat gtcgtagagg ggcgagacca
      781 taaaattgac ctggggcacc gcggaaggtg cgtcgggggc aaccagcaga ggcattttag
      841 cgactccttg tgtgtaggtt gagactacag aacgcagaga gactacaaaa gaagcaggct
      901 ctttgtgact aatgcgcaaa taggtgattg gcaattagcc aagcgtacaa cattctacat
      961 tgtcgcctgt tttattgtca atacgtaatt tgcaggtcaa gcgtcagccg aacgtgttat
     1021 ccgtggcccc ttatacactg cgacagggcg tccccacagg gtgtcacacc gctcgcgccg
     1081 agaataattg cccatgggtc ctgcgatgtg gagcaccagc caacgcgctc ctacggctgc
     1141 acgtaagcgt cggcctcaat ctccaccagc atatctgggg agattcgccg gcttacctcc
     1201 accatcgtgg cggcggggcg tatgttgcgg aagaactcgg catctgcccg gaccacctcc
     1261 tcccagcggc tgatgtccgt cacaaacatg cgagtgcgca ccacgtcggc catgccggcc
     1321 cctgccattt ggagcgcgcg ctcgatgtta cgcagcgtct gtatcgtctg cgcgtaagca
     1381 tcccccgccc ccaccacgtt gccctcagcg tcggtagccg tagtacccgc cacgtggata
     1441 tacggcccca cctgcaccgc cctcgaatac cccacaaccg gctcccaggg cgcgcccgtg
     1501 ctatagttct tcctctccac tctaacaccc ccacaaaaag atagagggca cacagcaaat
     1561 gtatgccctc tcccaaacca attaagaatt aagaattaac aattaagaat ttccctaggg
     1621 taccatctcc gtgaatacca ccaggcgctt ctcgtaattc tgggtggcgc ggttgaaggc
     1681 gccctggcag gtaatcaggt tgaggcgtgt ctgcttggtg gggccgaaca cgtccatcaa
     1741 ggggcaattg ttccagacgc acgtctgttg tttggtgact acgaaggcgc ggtcccggcc
     1801 cctgtcgtcg cgcacgtaca cgcggtcacc ggccttgagc ttgtcgaggt ggaagaagac
     1861 cgcctccggg atgccgtacc agttcaggtg gccgtccatc accgcattgc cggggttgcc
     1921 gggcaccgtg ccgggcttga accagccgac gttcatcgcc tccttgggga tgtccatgtt
     1981 gttgtctttg tccacgccca cgtactcgac cttggagacg acgcccatag tggggatcga
     2041 caggtagatg ggcaggctgg gcttggcggc aggcagcttc tcggggcctt tgccgtcagg
     2101 cccgtagcgc cactcgaagt agtgctgtcc tatgttgccc atctgcacct tccaacctgc
     2161 gggctggtca ggggcgtagg tgagcacgcg gcgctcaaac gcctggatga gcacttcctg
     2221 gctcttgccg ccgatcttga cgttagccca gtaggcatcg gagataggca ggcccatcgc
     2281 gaagacccac gggtcgagga agggcttgct ggccgtgccg atgccctggc gcacctgggc
     2341 cttgctgttg aggaagtccc agaagacgct cggtatgttg tgcccggtcg ccttgtcgta
     2401 atacacgatc ctggcctcgg caagcttggt cttggaggcg tcgtccgtca cgtcgcccgt
     2461 gcggctgatg cgctgggagg cgtagccgcc cttggtcttg tcgggctggc gcttctcgcc
     2521 cgccgaggtg ttggacacgg agccgaagga ggcgtacatc ggggcgttgc catcgtcggg
     2581 gtcggaggcc aggtttatgc ccgcgggctt gccggtgacg aacttgctgt tgcccacctg
     2641 catcaggccc gaaatcagct ccacggtcag caggccgttg gtgacgaagc cgttggcggt
     2701 gggggtgcct ttgggattgt tgatctccat gcgggccttg tcaaagtact gcaccaggcg
     2761 caggcgagtg ccgtcgggcg cttcttcgta cgcttcgaga cctgccgcca taggggccgg
     2821 cccccacgtc caggaccggg ccaccgattt ggcggccaca agctggtcgt tgcgcatcca
     2881 gacagcctcg aatgccgagt cggcgaacgg cagggaggcg ggaagggcag actgggcacc
     2941 ggcaggcgac tgcgacggcg cggcatacga ggaagccgaa g
//
LOCUS       DASRWZ010000311         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_26338,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000311 DASRWZ010000000
VERSION     DASRWZ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26338"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFH60_04845"
     CDS             complement(<1..903)
                     /locus_tag="VFH60_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313141.1"
                     /translation="MKARQRIQKGFSIAGFVVMALALAGQSSTAAFASTLGSGKVPSQ
                     PNAVQYVVNNTNDSGAGSLREAINMANAGPGKDSIIFNIPGSGVKTISPLSALPAITD
                     PVEIDGTTQPGYTGTPVIELNGSSAGWPQAGLVVSAGNSTIKGLVINRFNAPNIKLTT
                     NGGNTLEGNFIGTNPAGTASMMTVGTGILIDGTSSNVIGGTTNAARNLISGNGANGIV
                     FVETISYGTRANQVLGNYIGTDINGTAAISNTFNGVVMNNAHDNVVGSTAAGAGNLIS
                     GNGLNGVYIEMAIGARVRGNYIGTD"
     gene            complement(1359..2492)
                     /locus_tag="VFH60_04850"
     CDS             complement(1359..2492)
                     /locus_tag="VFH60_04850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6313142.1"
                     /translation="MSYPKMAVGLQVATGPPLGLSRAMVLLARLSRLDSVLAVDHFQG
                     FVPLALWDRELTWAAGQFSSPHEIFDYQVLLGYLARSAGRLRVGVGVTEPVRRHPVLI
                     AQAMMTLAHMTRRPPILGIGAGERENIEPYGLDFSHPVGQLEEALKIIRLCFSSRGPF
                     DFAGAHYRLDRAVMDLHPPKGRTPSIWIAGHGPRMLRLTGQYGDGWLPTMVASPQEYR
                     ARLEVIRAAAREAGRDPAAITPALHQFVAVAPTRREARAMLGTKVARFAALVGTPSAE
                     WRNVGREHPFGEQYRGYVDFLPETYDRKTLNEAIAAVPPQLVGRGFVIGTLDEVVQRL
                     REYGEAGARHVVISLSSAVFSRRAAVYGQLAVPRLAWLLRHGR"
BASE COUNT          482 a          809 c          863 g          582 t
ORIGIN      
        1 gtcggtgcct atgtagttgc cgcgcactct tgcgcctatc gccatttcta tgtacacgcc
       61 gttgaggccg ttacccgaga tcaggttacc cgcgcccgct gccgtgctac cgacgacgtt
      121 gtcatgggcg ttgttcatga ctaccccgtt gaaggtgttg ctaatagcgg cggtgccgtt
      181 gatgtcggtg ccgatgtagt tgccgagtac ctggttggcc cgggtgccgt agctgatggt
      241 ctctacaaag acgatgccgt tcgcaccgtt gccggagatg aggttgcgcg cggcgttcgt
      301 cgtgccgcca atgacgttac tggaagtgcc gtcgatgaga atgccggtgc cgacggtcat
      361 catgctggcc gtacccgcgg ggttggtgcc gatgaagttg ccttccaggg tgttgccgcc
      421 attcgtggta agcttgatat tgggggcgtt gaagcgattg atgaccaggc ccttgatcgt
      481 gctgttgccc gcgctcacca cgagtccggc ttgcggccag cctgcgcttg agccgttcag
      541 ctctatgaca ggcgtgcccg tgtagcccgg ctgcgtggtg ccgtcgattt ccaccgggtc
      601 ggtgatagca ggcagagcgg agagggggct gatggtcttc acgccgctgc cgggtatgtt
      661 gaatataatg ctgtctttgc cggggccggc gttcgccatg ttgatagcct cgcggagcga
      721 gcctgcgccg ctgtcgttgg tgttgttcac cacatactgg acggcgttag gctgggaggg
      781 aaccttgccg ctgccgaggg tggaggcgaa tgcggcggtg gaggactgcc cggcgagtgc
      841 caacgccatc accacaaacc ccgctatgct gaaccccttc tgaatgcgct gccttgcctt
      901 catggttatt gccttctcct ttgcgttttc tatatgttcg atgtttccta aatgcttgcc
      961 tggttgttta ctgtgtgcct ttttatgtga gctttctata tagtgcgtgc ctctgccggg
     1021 cttgttctca gtctatagca gcccgagcct ccatacttcc caaagaagta ctaaagctct
     1081 gacgacttag gttgtgtgcg cgtattacat acaaagatgt aatcgatgta ctcgcatgta
     1141 gtagcgtcac cctttcgata gggtgccctt tgtgcatacg gaaacaccaa ggcgccaagg
     1201 atcacgccaa ggtatccagg cttggaggat acaaaaggga ccagagtaca tgatttcgta
     1261 ctctggtcct gttagcactc acgacttaat cttaaacttt gcgcgttctt tgcgtccttt
     1321 gcgtctttgc gttttcgtct tgggccggtg ttactaaatt accggccgtg cctcaacaac
     1381 cacgcgagcc tgggtacggc taactggccg tacacggccg cccgcctgga gaaaacagcc
     1441 gacgagaggc tgatgaccac gtgccgcgcc ccggcctcac catactcccg cagcctctgc
     1501 accacctcgt caagcgtgcc tatgacgaag ccgcgcccca ccaactgggg cggtaccgcg
     1561 gctatcgcct cattgagcgt cttgcggtcg tacgtttctg gaaggaagtc aacgtaaccc
     1621 cggtactgct cgccgaaggg gtgctccctg cccacgttgc gccattcggc cgagggcgtg
     1681 cccaccagcg ccgcgaaacg ggccactttg gtgcccagca tggcccgcgc ttcccgccgg
     1741 gtaggcgcaa ccgcgacgaa ttggtgcagg gcgggagtga tagctgcggg gtcccgtccc
     1801 gcttcccgtg cggcggcccg gattacctcc agcctggccc tgtactcctg gggtgaggcg
     1861 accatcgtgg gcaaccagcc gtctccgtac tgcccggtga gcctgagcat gcgcgggccg
     1921 tggcctgcga tccagatcga gggcgtcctg cctttcggcg ggtgcaggtc catcaccgcc
     1981 cggtccaggc ggtagtgcgc gcccgcgaaa tcgaaagggc ctcgcgacga gaagcagagc
     2041 ctgatgatct tgagggcctc ctcaagctgg ccgaccgggt gtgaaaagtc caggccgtac
     2101 ggctcgatgt tctcgcgctc gcccgcgccg atgccgagga ttggcggtcg cctggtcatg
     2161 tgcgccaacg tcatcatggc ctgggcgatg agcaccggat ggcggcggac cggctcggtg
     2221 acccccacgc caacgcgcag cctacccgcg ctacgggcga ggtagcccag tagtacctgg
     2281 tagtcgaaga tttcgtgcgg gctggagaat tggccggcag cccaggtaag ctcgcggtcc
     2341 cacagggcga ggggtacaaa accctggaag tggtccaccg cgagcacgga gtcgaggcgg
     2401 ctcagcctgg caagcaggac catagcccgg ctcaggccga gcggagggcc ggttgctacc
     2461 tgcaacccga cagccatttt ggggtagctc atagccaatt ataccctcaa ccgccagagc
     2521 tacaagcata gctagcaggg tatagtcatt cgcggcaggg gacgtcctgc atgacgccgt
     2581 gggatagccc tgtggctaaa gccacgggct gggaagcagc aaagtctcct tcggagactg
     2641 gggagtatct gcaaagtgcc acccgtgtgg gaccagagtg gcccctgtca ttctgagcgt
     2701 agcgaagaat ctaaagaagg tggcacaggg aggctg
//
LOCUS       DASRWZ010000312         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1122923, whole genome shotgun sequence.
ACCESSION   DASRWZ010000312 DASRWZ010000000
VERSION     DASRWZ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122923"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFH60_04855"
     CDS             <1..409
                     /locus_tag="VFH60_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009786650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6313143.1"
                     /translation="NTAPTSFGSYDIPEGTSILWSPAVVHRRPDLYPEPNAFKPERWA
                     GIDPSPYEYMPFGAGPRRCLGAIFAIMEMKMVLPIILQRFGIQLPHNVQVDLGGSPLA
                     TPRGGLPARLTPARKVPAPARVRGNIRRLIDLP"
     gene            813..>2127
                     /locus_tag="VFH60_04860"
     CDS             813..>2127
                     /locus_tag="VFH60_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET6313144.1"
                     /translation="MLQRVTAWAAMLFVYLALAVGAGAATSQSVATQHGQRALLAGRG
                     HAPIARVYAPSRQTGGARFLTLPFTDPGVAVQQGWVYSWGSEHYGTDYVLGGPEIAKL
                     RQFDVVASADGYACGNCTSRQGNAVWIKHVIDGQTYYTYYGHLDTIESDIPVGNQQNT
                     VFVKRGQKIGVSGSTGASTVHLHFQVNRSSGPVDPYDLWSTNEPYMPGCTTCEMGPTN
                     LWTTNPPSFASGEALPGGPAGPPTRREQPQPTSQTRQDLPPQPAQPTRTPAPTRTPAP
                     PTPTATPTPVPCDLAYDDTVQGLLTDAKPQVEYCIRAEAAQWVSVKMFGVKDATLDTV
                     LKLYGPDGTLLASDDDGAQIGGNSFLVARLPKKGTYKLVAARYSGTGSYRVRAEKGAK
                     SALGDLNGDCRIDTTDTQALALALGTRDPKTDLNLDGVVDDLDRQA"
BASE COUNT          411 a          765 c          606 g          345 t
ORIGIN      
        1 caacacggct cccacgagct tcggctcgta cgacatcccg gaaggcacgt ccatactctg
       61 gagtcccgct gtggtccacc gccgccccga cctctacccc gagcctaacg ccttcaagcc
      121 cgaacgctgg gcgggcatcg acccctcccc ttacgagtac atgcccttcg gggccggccc
      181 gcgccgttgc ctgggagcta tcttcgcgat catggagatg aaaatggtgc tgcccatcat
      241 attgcaacgg ttcggcatcc aactgcccca caacgtgcaa gtggacctcg gaggctcccc
      301 cctcgctaca cccaggggtg ggctgcccgc ccgcctcaca ccggcacgca aagtacccgc
      361 accggccagg gtgcgcggga acatcaggcg tcttatcgat ctaccctgag cctgtggaat
      421 acggagggct ttgtcattcc gagcgtagcg aggaatcagg tggcagtcgt taattgccgc
      481 gagaggaaga cttaagcggc aagaccaccc cggtacagca atgcgacttc ttccctaccc
      541 acctgattcc tcgctacgct tggaatgaca aggctctctt cgcccctgct aatccagggc
      601 gtgtgacttg tcttcgcagt cctcgcagcc ctctcatgcg cgcgagtttt ccacaactga
      661 taggaactta gtactaaagc ttactccaaa gggttgacat atgactccgc cctcggatat
      721 agaacctcct gtgaacgcgc gaagctatgc gcgttccatt gcagctagcc cggctacgaa
      781 agccatgcag ccggtcgttt ggaggtggca atttgctaca gcgcgttacc gcttgggcgg
      841 caatgctctt cgtatacctg gcgcttgcgg ttggagccgg tgcggctacc tcgcaaagcg
      901 tagctactca gcatgggcag agggcgctgc tcgcaggcag gggccacgcg cctatcgccc
      961 gcgtgtacgc tcccagccgc cagaccggcg gcgctcgctt cctcaccctc cccttcaccg
     1021 accccggagt ggccgtgcag cagggctggg tatatagctg gggcagtgag cactacggca
     1081 ccgactacgt gctgggcggg cctgaaatcg cgaagctgcg gcagttcgac gtagtggcct
     1141 ccgcggacgg gtacgcctgc ggcaactgca cctcgcgcca gggcaacgcg gtctggatca
     1201 agcacgtcat cgacggccag acctactata cctactacgg ccacctggac actatcgaga
     1261 gcgacatccc ggtcggtaac cagcagaaca cggtgttcgt caagcgcggc cagaagatag
     1321 gcgtgtcggg cagcaccggg gccagcaccg ttcacctcca tttccaggtg aaccgctcct
     1381 ccggccccgt ggacccctac gacctgtggt ccaccaacga gccttacatg cccggctgta
     1441 ccacctgcga gatgggcccc acaaacctgt ggaccaccaa cccgcccagc tttgcctccg
     1501 gcgaggccct gccgggagga cccgcaggcc cacccaccag gcgagagcag ccccaaccga
     1561 cctcccagac gcgccaggac ctacctccgc agccggccca gcccacccgc acgcccgcgc
     1621 ctacgcgtac ccccgcaccc cccacgccca ctgccacccc tacgcctgtc ccctgcgacc
     1681 tggcatatga cgacacggtg caaggcctac tgaccgacgc gaagccacag gtcgagtact
     1741 gcatacgcgc ggaggccgcg cagtgggtct cggtgaagat gttcggcgtc aaagacgcaa
     1801 ccctcgacac ggtcctcaag ctgtacggcc cggacggcac cctgctggcc tccgacgacg
     1861 atggcgcgca gattggcggt aactccttcc tggtggcgcg gctgccgaag aagggcacct
     1921 acaagctggt agccgccagg tatagcggca caggctccta ccgggtgcgc gcagagaaag
     1981 gtgccaagag cgcgctgggc gacctcaacg gcgactgccg catagataca accgacaccc
     2041 aggccctcgc ccttgctctc ggcacccgcg accccaagac tgacctcaac ctcgacggcg
     2101 tggtggacga cctcgaccgc caggcca
//
LOCUS       DASRWZ010000313         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_437647, whole genome shotgun sequence.
ACCESSION   DASRWZ010000313 DASRWZ010000000
VERSION     DASRWZ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437647"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..>1921
                     /locus_tag="VFH60_04865"
     CDS             38..>1921
                     /locus_tag="VFH60_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET6313145.1"
                     /translation="MMGRGALPALPGGWRSVAAALGLVVFAVVGARAWQEGWRGARLR
                     AWAMSHPATLALGGITLGAFFLRVFGISDNLPYTSYPDEPAVADRALGILQTGDYNPH
                     YFVYPNLYTYMQAAVYAVRFFYLVSAGQLSNLDGITPTDFYLWGRLLTALLGALTVPL
                     VYLAARRLYGVTVGLIAAAFMATNSVHLVHSQLITTDVPATFYSALSLLAIVRLLPPG
                     EDLRADDGQGRLAPVSGYVAAGIAMGLAVGTKYNSALVLLPFLVAHAYAGADLPGERF
                     RRFLGARLWAGLGAMVATFLLTTPFIIFDLPNFLNEVASVITHYRFGHLGFEGDDNWR
                     FYTEIFLRTDTWPTLLTLGGVVLAFARHKRADMLLLVFPLAFYLSMSSYRVNFTRNLL
                     PMLPFTSILAAVPLALVGKALLARVLASRRVNFRLAARVAPWLLALLVTLLVLPMTAS
                     ALQRGYRQTQPDTRVRASQWLDANVRPGTKLWLEPQTPILTPGRYITGHGEHVTDNPP
                     DWYVANRYEYVVLSEGAYKDVVYDHPEANPPLRDAYLRFFADNESRLAASFESNKIDH
                     PGPSIRIYRTAYTPPRSAADVHAQYPLGASFHEVAANGGTVRLLGVDYPQVVDAGSVL
                     PLTL"
BASE COUNT          304 a          628 c          588 g          401 t
ORIGIN      
        1 atacgtcgtg tttctagcca ggaatgacat tgacggtatg atggggcggg gcgctctgcc
       61 cgctctcccc ggcggctggc gaagcgtggc ggctgcactg gggcttgtgg tcttcgcagt
      121 ggtgggtgcg agggcgtggc aggaaggttg gcggggtgcg agactgcggg cgtgggctat
      181 gtctcaccca gccacactcg cgcttggtgg catcacgctt ggcgcgttct ttttgcgcgt
      241 cttcggcatc agcgacaatc tcccctacac cagctacccc gacgagcctg ccgtagcgga
      301 ccgcgcgctc ggcatcttgc agaccggcga ctataaccct cactatttcg tctaccccaa
      361 cctctacacc tatatgcagg cggcggtcta cgcggtgcgc ttcttctacc tggtctcggc
      421 aggccaactc tccaatctag acggcattac gcccactgac ttctacctgt ggggccgcct
      481 gctgaccgcc ttgctcggcg cgctcacggt gccgctcgtc tacctggcgg cgcggcgctt
      541 gtacggcgtg actgtgggcc ttatcgccgc cgcgttcatg gcaaccaaca gcgtccacct
      601 ggtccattct caactgataa ccaccgacgt gcccgcgacc ttctacagtg cgctgtccct
      661 gctggctata gtgcggctct taccgcccgg cgaagacctc agggcagatg atgggcaggg
      721 ccgtttagcg ccggtaagcg ggtatgtggc ggcgggcatc gctatggggt tggcggtggg
      781 caccaagtac aatagcgcgc tggtgctgct gcccttcctg gtggcgcatg cctacgcggg
      841 agcagacctg cctggggaga ggtttcggcg cttcttggga gcgcgcctgt gggcgggact
      901 gggcgcgatg gtcgcgactt tcctgctcac cacgcccttc atcatattcg acctgccgaa
      961 cttcctcaac gaagtggcct cggtaattac gcactacagg ttcggccacc tgggcttcga
     1021 gggcgacgac aactggcgct tctacaccga gattttcctg cgtaccgata cgtggccgac
     1081 gctgctcacc ctggggggcg tagtcctcgc atttgcgcgg cataagcggg ccgacatgct
     1141 gctgctggtc ttccccctgg cattttatct ctcgatgagc agctaccggg tgaacttcac
     1201 gcgcaacctg cttcctatgc tgcccttcac ttccatcctg gccgccgtgc ccctggcgtt
     1261 ggtgggtaag gctctgctgg cgcgcgtgct tgcttcgagg cgggtcaatt tcaggcttgc
     1321 cgcgcgagtc gctccctggc tgcttgcgct gctggtgaca ttgttggtgc tgcccatgac
     1381 tgcgtccgcc ttgcagcggg gctaccgaca gacgcagccc gacacccgcg tgcgcgcatc
     1441 gcaatggctc gatgccaacg tcaggcccgg cacgaagctg tggcttgagc ctcaaacgcc
     1501 tatcctgaca ccgggccgct atatcaccgg gcatggcgag cacgttaccg ataatccacc
     1561 cgattggtac gtcgccaaca ggtacgaata cgtggtgctg agcgagggcg cgtacaaaga
     1621 tgtcgtgtac gatcacccgg aggcaaaccc gccgttacgc gatgcgtacc tgcggttctt
     1681 tgccgacaac gagtcgcgcc tggccgctag cttcgagtcg aacaagatag accacccagg
     1741 ccccagcatc cgcatctacc gcaccgctta taccccgcca cggagcgcgg ctgacgtgca
     1801 tgcccaatac ccactcggcg cgtcgttcca cgaggtcgca gcgaacggtg gcacggtccg
     1861 cctactaggt gtggattatc ctcaagtcgt agacgcgggt agcgtgctgc ctctcaccct
     1921 c
//
LOCUS       DASRWZ010000314         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_437805, whole genome shotgun sequence.
ACCESSION   DASRWZ010000314 DASRWZ010000000
VERSION     DASRWZ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437805"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="VFH60_04870"
     CDS             complement(<1..1540)
                     /locus_tag="VFH60_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313146.1"
                     /translation="MAAVYFFARRALSPGAALVALALLVLCPITIWFARYPVSEVLMG
                     VLVFSACLGFLRMVQLTREPQVPAHSTAAQDEPVQAGGDGRNTWAALWGAIAGVSLGE
                     MALARPDFIFYLAPLPAYLIYWRLTRRWQRAHTWLLGSLAVVLGVYLVHLSFYGFAYT
                     LDVYHNVILDTRRRWGPLLLALYAGSIGLVALDRLHPRLAPLWVRLENLAVRYRWLWA
                     GALMLLVGGYTAYHYLVGPWLPNPRFTDAGEALPQLVTTTWESYIGAPVDLGGRYNLL
                     RIGWYLSPPAMLLAILGLLRWIWTRLNAATGLFFAMLLALSYVFIQETYTDAHFIYTM
                     RRYVPIILPALVLGVAWACQWLWSRLRPRTVGAALAGVIILGLGTFYIYTGRAIIPHV
                     EERGAVAQLSELASRFPNPARTVVLFSDGRDEPYLISTPLHFIYGIESFVLVRQYPNI
                     RSDIVEQVVKRWQSEGWEVYLMMGVNGGKAHFADLSLREMGSWDYDVDEFQQLYYQKP
                     ANVSR"
     gene            complement(2303..>2889)
                     /locus_tag="VFH60_04875"
     CDS             complement(2303..>2889)
                     /locus_tag="VFH60_04875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313147.1"
                     /translation="GLEQGMFIGLSVARLEANKGLPYLLRALRRMPPEIEWRWVILGS
                     GTDRAALQGEAAGLGISERVIFAGSLPDADLHNLYELFDLFGHSTLYEGSSLVTLEAM
                     SHGLPVIASAVGGIPDKVIEGETGFLVSPGDTSQLAARIAELATRPGERRALGERGAA
                     RVEAEFSWRSIAAQTELLLRRLIDVRQACRSKEV"
BASE COUNT          563 a         1012 c          901 g          413 t
ORIGIN      
        1 cgcgcgacac atttgcgggc ttctggtagt acaactgctg gaactcgtcc acgtcgtagt
       61 cccagctacc catctcccgc agtgataggt ccgcgaagtg cgccttgccc ccgttcaccc
      121 ccatcatcag gtagacctcc cacccctcac tctgccaccg cttgacgacc tgctcgacta
      181 tgtcgctgcg gatgttcggg tactggcgca ccagcacgaa gctctcgatg ccgtagatga
      241 agtgcaaggg agtcgaaatg aggtaaggct cgtcgcgccc gtcgctaaag agcaccaccg
      301 tcctcgcggg gttggggaag cgggaggcca attcgctcag ttgcgccacc gccccgcgct
      361 cctcgacgtg ggggatgatg gccctgcccg tgtagatgta gaaggtgccc agccccagta
      421 ttatcacacc cgcaagggcc gcacccaccg tcctgggccg caggcgcgac catagccact
      481 ggcacgccca cgccaccccc agcacgaggg cgggaaggat aatcggcacg tagcgccgca
      541 tggtgtagat aaagtgcgcg tcggtgtagg tctcctgtat gaagacgtag ctgagtgcca
      601 ggagcatagc gaagaatagg ccggtagcgg cgttgagccg ggtccatatc cagcgcagca
      661 ggccgagtat ggcaagcagc atagcggggg gcgagaggta ccagccgatc cgcagcaagt
      721 tgtaccggcc gccgaggtcc acgggtgccc cgatgtagct ctcccaggta gtcgtcacga
      781 gttgcggaag cgcctcgccc gcatcggtaa agcgcgggtt gggcagccac ggcccgacca
      841 gatagtggta cgccgtgtag ccgcccacca ggagcatcag cgcgcccgcc cacagccacc
      901 tgtagcggac tgccaggttc tccaggcgca cccagagcgg cgcaaggcgc gggtgcagcc
      961 ggtcgagcgc cacaagcccg atcgagccgg cgtagagggc caggagcagc ggcccccacc
     1021 tcctccgggt gtccaggatc acgttgtggt acacgtcgag cgtataagcg aacccgtaga
     1081 agctcaggtg caccaggtag acgccgagca ccaccgccag gctacccagc agccacgtgt
     1141 gcgcccgctg ccagcgccgt gtgagccgcc agtaaatcag gtatgcgggc agcggcgcga
     1201 ggtagaagat gaagtcgggc cgggccaggg ccatctcgcc aagcgatacc cccgcgatgg
     1261 ccccccacag agcggcccag gtgtttcgcc cgtcgccccc ggcctgtaca ggctcatctt
     1321 gggccgccgt ggagtgcgct gggacctgcg gctcgcgtgt tagctgcacc atgcgcagaa
     1381 agccgaggca cgcgctgaaa acgagcacgc ccatcagcac ttccgacacc gggtagcgtg
     1441 cgaaccagat cgtgatgggg cagagcacca gcagcgccag ggcgaccagc gccgcccccg
     1501 gactcagcgc ccgcctcgcg aagaaataga ccgccgccac ccccagcaac gccaacagcg
     1561 agttggcgta cagccccccc ccagatgccg aacaggctgt agagcagccc tatccagaca
     1621 ggcaggagcg agtagtgctg cggcatcacc agcgcggcct gcttgtccag cacgtagaag
     1681 ccggccatgc gcaggttggc tagcgtgtag cggtcgcggg acagcccttg cagcaactcg
     1741 ttggtctcgc ccttgtcggt gtcgaggtag cgcatgagca tgtcgcgctg gaaaatcgtg
     1801 ccgctccgcc ctagcgccac cccggcgttc acgtataccc cggagtcgag cgcacctcgc
     1861 aacatctcgg cgggacgcga gaccagcccc gcagccacca acaacacgac gaccaggcca
     1921 atggtgtgca agcgggcgaa acggcgctcc tccgcgcgtt gcggttgggg ggcaggcggc
     1981 agcaggaggc gagacagcgg ggcgagccgc agactctgcc cacccaccgt gacggctatt
     2041 accaggctga atgcaaccag gattgccagc agcaaccagg ggcggaacat gcccatctcg
     2101 gcaagcgcca gccctacgcc cccggtcagc aggaagctga cgagcaccgc cgtgaagagc
     2161 cattcatccg tccccgaaaa gccgtgcctg cgggcagacc tcagcgccag gcagttgaga
     2221 agcacggccc ccggcgcgta gagcatgtac agcagcgccg ggatgcccgt gagcaggtta
     2281 agtatctcca acagggacgt aactagacct ccttggagcg acacgcctgc cgcacgtcaa
     2341 tcaaacgccg caatagcagc tcagtctgcg cggcaatgct ccgccagcta aattccgcct
     2401 ccaccctggc agcgccccgc tctcccagcg cccgccgctc cccagggcga gtcgcgagtt
     2461 ccgcgatcct ggccgcaagc tgcgaggtgt cgccggggct gacgagaaaa cccgtctctc
     2521 cctcgattac cttgtccggt atgccgccca ctgcgctggc aatcaccggc agaccgtgcg
     2581 acatggcctc cagcgtgacc agcgagctgc cctcgtacaa cgtcgagtgc ccgaacaagt
     2641 cgaacagctc gtacagatta tgcaggtcgg cgtcgggcag gctgccggcg aagattacgc
     2701 gttcggagat acccagccct gccgcctccc cttgcagcgc cgcccggtct gtcccgctgc
     2761 ccaggatcac ccagcgccac tcgatctccg gcggcattcg ccgcagcgct cgcaggaggt
     2821 agggcaggcc cttgttcgcc tccagcctgg ccacgctcag cccgatgaac atcccctgct
     2881 ccaggccca
//
LOCUS       DASRWZ010000315         4685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_711933, whole genome shotgun sequence.
ACCESSION   DASRWZ010000315 DASRWZ010000000
VERSION     DASRWZ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711933"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1726
                     /locus_tag="VFH60_04880"
     CDS             50..1726
                     /locus_tag="VFH60_04880"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HET6313148.1"
                     /translation="MNTAKKLNVALAGLMVAGVAAFGGNAGQAAASSHREAPMISQDP
                     VADGTDVYAFVSPDRPDTVTLIASYYPFEEPAGGPNYFRFGDDVMYDIHVDNVGDAKA
                     HVTYRFDFSTTVANGNTFLYNTGPIESIDSPNLNVKQTYSVTEIRNGQSKMLGENLKV
                     PPVNVGAKSTPNYEALSNAGIQDLGDGSRVFAGQSDDPFFVDLNVFDLLTIRKLPGNA
                     GGGVDGLKGYNVQTLAIQVPMRNLTSDAQAVSGPTGTNAVIGVWSTASRQSTRVLNAN
                     GTVQNSGDMVQVSRLGAPLVNEVVVPLAAKDLWNGSTPEGDAQFLGGVTDPELAKLFK
                     ALYNIQVPPAPRDDLVAIFLTGIPGATQPPTSTLKPSEQLRLNMGVPPAAQPNSLGVV
                     GGDMAGYPNGRRLADDVTDISLKAVAGAAYPLFHPDFTPDPLAAQLGDGVDANDVAFR
                     TSFPYVALAHSGTASVPHGVEAPAAAAAPAPAPAAGNAGGSQAGGAASMGAGQAPGMP
                     RTGTARGIFEIEHLADIYGYVLAAIAGMGGLIMVGGWAMRRKAAETTTKK"
     gene            1810..3159
                     /locus_tag="VFH60_04885"
     CDS             1810..3159
                     /locus_tag="VFH60_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6313149.1"
                     /translation="MEASLAHNKHRIQNNGTLILGVVLLVIAAGLLVKPFMPAPAVSS
                     SPTVDAEALLATGGQATDRYTDRVIRDMQARLKSEPGDYKAHASLGVAYQQKARETND
                     PAYYSEAEKSFIQALQLKPDDYNSLAGMGALELSRHNFTSALDWANRAQRVIPDKAYA
                     YGVAGDAHIELGNYDEAVAAFQKMVDLRPDLSSYARVSYARELHGDVDGAIQAMQQAV
                     AAGGPAAENTAWCRVQLGHLFFNSNRLDDAEKTYVDALAGYPGFTHAQAGLAQVRWAQ
                     GKTAEAIDLYKQANASVPLPQYIAALGDIYASTGDKQAATEQYDLVSYIYDLQRKGGV
                     VVDVEQAAFYADHDMQLDEALKLAQTAAQGRNDVNTLDTLAWALYKNGRYEEALSAQR
                     KAMRLGTRNAAFYYHLGMIQKALGMTDEARISLEIAMAINPNFSPIYSPQARAFLDQ"
     gene            3190..>4685
                     /locus_tag="VFH60_04890"
     CDS             3190..>4685
                     /locus_tag="VFH60_04890"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313150.1"
                     /translation="MNKGTRAATVLAVLVCLVGILAPRPAAAHPMGNFSINRYSAITV
                     GADRVDLHYIVDMAEIPAFSELATIREDRSTDLSDTERQGYIARKSAEVRSNLHMNVG
                     GKDVPIELVSASLLFPPGQFDSPTLRLDLKFEGAIAASGGATLQYRDDNFAERLGWKE
                     VVASSGEGVRLVSSNVPSVDVSKGLTDYSLEAVEARPNVTSAEIAFEPGTAAATAAQD
                     SVVTQQNKGGLDFGAWIRDQEQVLTGLLTNNDLPLGLLLAGLGLAFAMGAAHAMSPGH
                     GKTIVAAYLVGTRGTPRHAVFLGLTVTFTHTIGVFVLGVVVLSLQQYVVPETIYPWLG
                     FGSGVLIMLVGISLFVQRFRYWQRSKVAAAGAVHTHDGHTHSHDDAAAHPHDHDHDHD
                     HEHEHSHDHSHGHSHDHTHDHNLAEPHSHGPFSRPHTHLPANGQPVTLGSLLALGISG
                     GIVPCPSALVVLLASIRLARVDLGLLLILAFSLGLAVVLTGIGLLTVWG"
BASE COUNT          917 a         1495 c         1487 g          786 t
ORIGIN      
        1 caagatagtc cgctccgcac agacgcggca gcgaggagga gaactttcaa tgaatactgc
       61 aaagaagctc aacgtagcgc ttgcagggct gatggtagcg ggagtagccg cgttcggtgg
      121 gaacgcaggc caggccgccg cctccagcca cagagaagca cccatgatta gccaggaccc
      181 ggttgcagac ggcacggacg tgtacgcgtt cgtcagcccc gaccgccctg ataccgtaac
      241 tcttatcgcc agctattacc ccttcgagga accggcgggc ggccctaact acttccgctt
      301 cggcgacgac gtgatgtacg acatccacgt tgacaacgtc ggagatgcca aggcgcacgt
      361 tacctacagg ttcgacttca gcacgaccgt ggccaacggc aacaccttcc tgtacaacac
      421 cgggcctatc gagagcatcg actcgcccaa cctgaacgtc aagcagacct acagcgtgac
      481 cgagattcgc aacggccaga gcaagatgct cggtgagaac ctcaaggtcc cgcccgtgaa
      541 cgtcggcgcc aagtctaccc ccaactacga ggcgctttcc aatgccggta tccaggacct
      601 gggcgatggc agcagggtct tcgccggaca gtcggacgac ccgttcttcg ttgacctcaa
      661 cgtgttcgac ctgctcacga tccgcaagct gcccggcaac gccggtggtg gcgtggacgg
      721 cctgaagggc tacaacgtgc agacgctggc catccaggtg ccgatgcgga acctcaccag
      781 cgacgcgcag gcagtctccg gcccgaccgg caccaacgct gtgataggcg tatggtctac
      841 ggcctcgcgc cagtctaccc gcgtgctcaa cgctaacggc acggtgcaga acagcggcga
      901 catggtacag gtaagccgcc tcggcgcgcc cctggtgaac gaagtggtag tgcctttggc
      961 cgccaaagac ctgtggaacg gctccacgcc ggaaggcgac gcccagttcc tgggtggcgt
     1021 gaccgacccc gagcttgcca agctgttcaa ggccctctac aacatccagg tgcccccggc
     1081 accccgcgac gacctggtgg ctatcttcct gaccggtatc cccggcgcga cccagcctcc
     1141 tacctcgacg ctcaagccgt ccgagcaact gcgcctgaac atgggcgtgc cgcccgcagc
     1201 ccagcctaac tcgctaggcg tggtaggtgg cgacatggcc ggctatccca acggacgccg
     1261 ccttgctgac gacgtgaccg acatctcgct caaggccgtg gccggggcag cctacccgct
     1321 gttccacccg gacttcacgc ccgacccgtt ggccgcgcag cttggtgacg gcgtggacgc
     1381 caacgatgtg gcgttccgca ccagcttccc ctacgtggcg ctggctcaca gcggtaccgc
     1441 ttctgtgcct cacggcgtag aggctcccgc cgcagccgcc gctcccgctc ccgcacccgc
     1501 cgcaggtaac gccggtggct cgcaggccgg tggcgctgct tcgatgggcg ctgggcaagc
     1561 cccaggcatg cccaggacgg gcaccgctcg cggtatcttc gagatcgagc acctggccga
     1621 catctacggc tacgttctcg cggcgatcgc cggtatgggt ggcctgatca tggtgggtgg
     1681 ctgggcgatg cgccgcaagg ccgccgagac gaccaccaag aagtaagttg accctttagg
     1741 gcgagggcgg gcttgtggcc cgccctcgcc actctctcgt tcgtaccgtt caaagaaggt
     1801 ggagtaagga tggaagcaag tctcgcacac aataagcatc gtattcagaa caacggcacg
     1861 ctcatactgg gagtggtgct actggtgata gcggcggggc tgctggtcaa gccgtttatg
     1921 cccgcacccg ccgtgtcctc aagcccgaca gtggatgcgg aagctctact ggcgacgggg
     1981 ggccaagcga ccgataggta caccgaccgg gtgattaggg acatgcaggc gcgcctcaag
     2041 tcggagcctg gtgactacaa ggcccatgct tccctcggcg tggcttacca gcagaaggca
     2101 cgcgagacca acgacccggc atattatagc gaagcagaaa agtcattcat acaggccctt
     2161 caactgaagc ccgacgacta caattccctc gccggcatgg gtgccctcga actgtcgcgc
     2221 cacaatttca cttcggcgct ggactgggcg aacagggcgc aacgagttat ccctgacaag
     2281 gcctatgcct acggggtggc cggagacgcg cacattgagc ttggcaacta cgacgaggcg
     2341 gtcgcggcct tccagaagat ggtcgatctt cgacccgacc tcagctctta cgcacgcgtg
     2401 tcttacgccc gcgagctgca cggcgatgta gacggcgcga tacaggcgat gcagcaggcc
     2461 gtcgcggcgg gaggcccggc ggccgagaat acggcctggt gcagagtgca gttgggccac
     2521 ctgttcttca acagcaaccg gctggacgat gccgagaaaa catacgtcga tgcgctggcg
     2581 ggttaccccg gctttacgca cgcacaggcg ggattggctc aggtcaggtg ggcgcaggga
     2641 aagacggccg aggccatcga cctgtacaag caggcgaacg cgagcgtgcc cctcccacaa
     2701 tatatcgcgg cgctgggtga catttacgcg agcaccggcg acaagcaagc cgcgacggag
     2761 caatacgacc tcgtaagcta tatctacgac cttcagcgca aaggcggggt ggtggtcgat
     2821 gtcgagcaag ccgcctttta cgccgaccat gacatgcaac tcgatgaggc gctgaagctg
     2881 gcccagacgg cggcacaggg gcgcaacgac gtgaacacgc tcgacacgct ggcctgggcg
     2941 ctttacaaga acggccggta cgaggaggcc ttgagcgctc aacgcaaagc catgcggctg
     3001 ggcacccgta acgcggcctt ctactaccac ctgggcatga tacagaaagc gctgggcatg
     3061 actgacgaag cccgcatcag cttagagatt gcgatggcga tcaatccgaa cttcagcccc
     3121 atctattcgc cccaggcaag ggcgttcctg gaccagtagg ccggtatgcg cgaagggagt
     3181 acgcacgcaa tgaacaaggg taccagggcc gccacggtcc tcgccgtatt ggtctgcctg
     3241 gtgggtattt tggccccacg gccggccgcc gcccacccga tgggcaactt cagcatcaac
     3301 cgctacagcg ccattacggt gggcgcggac cgggttgacc tgcactacat agtggacatg
     3361 gccgaaatcc cggccttctc ggaactggcg accatacgcg aggaccgtag caccgacctg
     3421 tcggacaccg aacggcaggg ctatatagcg cgcaagtcgg ctgaagtccg gtccaatctg
     3481 cacatgaacg tcgggggcaa ggacgtgcct attgagttgg tgagcgcctc tctattgttc
     3541 ccgcctgggc agttcgactc gcccacgttg cgcctggacc tcaagttcga gggtgcgata
     3601 gcggcgagcg gtggtgccac cttgcaatac cgggacgaca acttcgcgga gcgccttggt
     3661 tggaaagagg tagtggcaag ctcgggcgag ggagtgcggc ttgtaagttc aaacgtgccg
     3721 tctgtcgacg taagcaaggg ccttaccgac tacagccttg aagcggtgga agcccgcccg
     3781 aacgtcacga gcgcggagat agctttcgag ccggggacgg cggcagcgac ggcggcacag
     3841 gactctgtgg tgacgcagca gaacaagggt ggcctcgact tcggggcgtg gatacgtgac
     3901 caggaacagg tgctcacggg cctgctcacc aacaacgatt tgccgctcgg actgctgtta
     3961 gcgggactgg ggctggcctt cgcgatgggg gcggcgcacg ccatgtcgcc cggccacggc
     4021 aagaccatcg tggcggctta cctggtgggt acgaggggca cgccgaggca cgcggtgttc
     4081 ctggggctga cggtgacgtt tacgcacacg atcggggtgt tcgtgctggg cgtggtggtg
     4141 ctatcgttgc agcagtacgt ggtgccggag acgatctacc cctggcttgg ctttgggtcg
     4201 ggcgtgctca tcatgctggt aggcatatcg ctcttcgtgc agcgtttcag gtactggcag
     4261 cggtcgaagg ttgcggcagc cggggcggta catacgcatg acgggcacac ccattcccac
     4321 gacgatgcag ccgcacaccc tcacgaccac gaccacgacc atgaccacga gcatgagcac
     4381 tcccacgacc actcgcacgg ccatagccac gatcataccc acgaccacaa cctggctgag
     4441 cctcactcgc atgggccatt cagccgcccg catacccatt tgcccgccaa cgggcagccg
     4501 gtgacgcttg gcagcctgct ggcgctgggc atcagcggcg gcatcgtgcc ctgccccagc
     4561 gcccttgtgg tgttgctggc ttccatacgg ctggcgcggg tggacctggg cctgttgttg
     4621 atactggctt tcagcctggg cctggcggtg gtgctgaccg gcatagggct acttaccgtc
     4681 tgggg
//
LOCUS       DASRWZ010000316         4365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_849458, whole genome shotgun sequence.
ACCESSION   DASRWZ010000316 DASRWZ010000000
VERSION     DASRWZ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849458"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /gene="accA"
                     /locus_tag="VFH60_04895"
     CDS             complement(<1..817)
                     /gene="accA"
                     /locus_tag="VFH60_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET6313151.1"
                     /translation="METERKQDTANTDVSNGSHQGLEALRQAVPPGISHKPDGAHTNG
                     ATNPVSSQAAQTSNAAPRGTQAEVNAWQRVQLARHPDRPHTLDYIQRICTEFVELHGD
                     RLFGEDASIIGGLGCFNGETVMVIGQQKGRSTRENIARNFGMPQPEGYRKAKRLMEHA
                     ARFGFPILTFIDTPGASPGVPSEERGIGQAIAENLVTMLDLEVPLIATVLGEGGSGGA
                     LAIGLGDRILMLENSIYSVASPEAAATILWRDASHAPAAAHTMRITAADLLEFG"
     gene            complement(870..1751)
                     /gene="accD"
                     /locus_tag="VFH60_04900"
     CDS             complement(870..1751)
                     /gene="accD"
                     /locus_tag="VFH60_04900"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET6313152.1"
                     /translation="MQDIFRKRRYFSLGQDKRPDTRGQAAPNREFPDDLWTKCPKCNE
                     LLYTRELENNLKVCQKCGYHFRLSSEERIDFLADEDTFVRGGPDVAPVDAIGFVSLEQ
                     KYSTKLKETQARTGLTDAVVYGSIRVEDVPAVVFVTDFRFFGGSMGSVYGQGLVEAAE
                     LAIDKKVGLLTVSASGGARMQEGIFSLMQMAKTTAILRELAEARLPHISLLTDPCTGG
                     VTASYATSADIVMAEPGALIGFAGPRVIEQTIKQKLPPDFQTAEFLLKHGMVDMVTPR
                     RELRTTASRLLRLYGNN"
     gene            2212..2445
                     /locus_tag="VFH60_04905"
     CDS             2212..2445
                     /locus_tag="VFH60_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313153.1"
                     /translation="MAEEAEQTGQDETNAVDENRRQLRSNFDAINQTAKDTRQKTRDL
                     YAGRDNTENERWGRREADSEYVNEVGDNDSSGG"
     gene            complement(2537..>4365)
                     /locus_tag="VFH60_04910"
     CDS             complement(2537..>4365)
                     /locus_tag="VFH60_04910"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET6313154.1"
                     /translation="CGPLPRKDMDIMRISRKPLTRIASTLVLVAATMLAVLPGSAALS
                     APAAQDDGTEGLPPGVRVETVLSDMDRPIAMAFDPAGRLFYTEKVTGNVRLFAGGKLQ
                     PNPVIHFDVSTNSERGLLGIAIDPTFNQNRFIYIYHTAPGSNTENRVVRFVERDGVGS
                     NPTVIFASPQTAGNHDGGNLHFGPDGMLYVSVGDNANADNAQDLTTKNGKIHRLNPDG
                     SIPADNPSWNVPGAVGSIYAYGLRNSFDFTFDPVVRGRIFASENGPGCDDEMNRIERG
                     FNYGWRANYPCDDANPPDDVNTIPPLWYQARSACCEAPTGIEVYDGWQIPEWRNHLFM
                     AAYQGGKLRHFYLNEDRTDVTRTNIVQGVDVNMDIETGPDGALYFMEGGGYSPGTLKR
                     FVGPGQPPTTNTPVVTPATTPVPGGNPSIPGSGSRTFPETRQTVTGLFLEYWDKNGGL
                     PQQGFPISGLINEVSDLNGKPYVVQYFERAVFEYHPENQAPYNVLLSQLGTFQYRKKY
                     PDGAPGQQPNTSAGSVLFPETGKRVGGRFLQYWQQNGGLPQQGYPISDEFMEKSDLDG
                     KTYRVQYFERAVFEMHPENQPPFDVLLSQLGKFRYDEKYGGR"
BASE COUNT          741 a         1295 c         1480 g          849 t
ORIGIN      
        1 cgccgaactc aagcaggtcg gcggcggtga tgcgcatggt gtgcgctgcg gcgggggcat
       61 ggcttgcgtc tcgccacagt attgtagcgg cggcttcggg gcttgccacc gaatagatcg
      121 agttttccag catcaggatg cggtcgccca agccgatagc cagcgcgccg ccgctgcccc
      181 cctcgcccaa caccgtggct atcaggggca cttccaggtc gagcatcgtc acgaggttct
      241 ccgcgatggc ctgccctatc ccgcgctcct cggagggcac gccggggcta gcgccggggg
      301 tatcgatgaa ggtgaggatg gggaagccga agcgggcagc gtgctccatc aggcgcttgg
      361 ccttgcggta gccctccggc tggggcatgc cgaagttgcg ggcgatgttc tcgcgggtcg
      421 agcggccctt ctgctggcct atgaccatca ccgtctcccc gttgaagcag ccaagcccgc
      481 cgatgataga cgcatcttcc ccgaaaagcc tgtcgccgtg cagctccacg aactcggtgc
      541 agatgcgctg aatgtagtcg agggtgtggg ggcggtcggg gtggcgggcg agttgcacgc
      601 gctgccaggc gttcacctcc gcctgggtgc ccctgggagc cgcgttgctg gtttgggcag
      661 cctgggacga gacagggtta gtggccccgt tggtgtgagc gccgtccggc ttgtggctga
      721 tgccgggggg cacggcctgg cgcagagctt cgaggccctg gtggctgccg ttgctcacgt
      781 ccgtattcgc ggtgtcttgc ttgcgctcgg tctccataag cctcgatgcc gtgcgaggta
      841 cgggggcttc ccggcctcgc atgtaaagtc taattgttgc cgtatagtct cagcaagcgg
      901 cttgccgtgg tgcgtagctc gcggcggggc gtcaccatat cgaccatgcc atgcttcagg
      961 aggaactcgg cggtctgaaa gtcgggcggc agcttctgct tgatcgtctg ctctatcacg
     1021 cgggggcctg cgaagccgat gagggcaccc ggctcggcca tgacgatgtc ggcggaagtg
     1081 gcgtaagaag cggtcacgcc gccggtgcag gggtcggtga gcagcgaaat gtgcggcagg
     1141 cgggcctcgg caagctctcg gaggatagcg gtggtcttag ccatctgcat gagcgagaaa
     1201 atgccctcct gcatgcgcgc cccgcccgaa gccgacacgg tgagcaggcc caccttcttg
     1261 tctatcgcaa gctcggcggc ctccaccagc ccctgcccgt agaccgaacc catgctgccc
     1321 ccgaagaagc ggaagtcggt cacgaagacc acggcgggca cgtcctcgac gcgtatgctg
     1381 ccgtatacga ccgcgtcggt cagcccggtc ctggcctggg tctctttgag cttggtggag
     1441 tacttctgtt cgagggagac gaagccgatg gcgtccacgg gggccacgtc ggggcctccg
     1501 cgcacgaagg tatcctcgtc ggccaggaag tcgatgcgct cttcggacga caggcggaag
     1561 tggtagccac acttctggca gaccttcagg ttgttctcaa gttcgcgggt atagagcagt
     1621 tcgttgcact tgggacactt ggtccacagg tcatcgggga actctctgtt cggtgcggcc
     1681 tggcctctcg tatcagggcg cttgtcctgc cccagcgaga agtagcgacg cttgcgaaag
     1741 atatcctgca aggttagctc ctggtgtgaa tcctgctggt cgatagccgg attttggtat
     1801 tataccacaa tcgcgacaag tgcttctgtt gcgcggatgt gctgccctat cgccctgtgg
     1861 gggtgccctg ccaatgaatt ggcgggctta ccagagacga agtctccttc ggagactacc
     1921 ctcacgctgc ttagtccctc atgggagttt gcctcttgta agcctcccac ttttgtgtca
     1981 cggcctccca ccgggacgaa cgacaaccca atcatacccc gtatacctcc atgcggtgag
     2041 ccgcaccatc cccatcacag ccagtacagg tacacaggca caactcttgc aaccaagcgc
     2101 gaaccagagg ctcaaccggc agtaaaagcc cgcactgagc ctgaggacaa gcaggccgcg
     2161 ttcgctacag gacttgtgtg ccagtagtct aagcacgagg gggatacaat catggcagaa
     2221 gaagcagaac agaccggcca ggatgaaacc aacgcagtgg acgagaacag acgccaactg
     2281 cggagcaact ttgacgcgat aaaccagaca gcaaaggata cgcgccagaa gacgcgcgac
     2341 ctgtacgcag gcagggacaa caccgagaac gagcgctggg gcaggcgtga ggccgactcg
     2401 gagtatgtca acgaagtcgg tgataacgac tcttccggcg gctagctgct gccacgcgct
     2461 tcttagcgtt taaacaagaa caggaggacg cgtgcgcgtc ctcctgcatc gcctggaacc
     2521 tgacggcggc gggatattac ctgccgccgt atttttcgtc gtagcggaat ttgcccaact
     2581 gcgagagcaa tacgtcgaag ggtggctggt tctccgggtg catctcgaac accgctcgct
     2641 cgaagtactg caccctgtac gtcttgccgt caaggtccga cttctccatg aactcgtccg
     2701 agatggggta gccttgctgc ggcagtccac cgttctgctg ccagtattgc aggaaccggc
     2761 ctcctacccg cttgcccgtt tcggggaaga gcacggagcc tgccgacgtg ttgggttgct
     2821 gtccgggcgc gccgtcggga tacttcttgc ggtactggaa cgtgcctagc tgtgagagca
     2881 gtacattgta cggtgcctgg ttctcagggt ggtactcgaa caccgcgcgc tcgaagtact
     2941 gcaccacgta aggcttgccg ttgaggtccg acacctcgtt gatgagtccg gagatgggga
     3001 agccctgctg gggaaggccg ccgttcttgt cccagtattc gaggaagagg cccgtcacgg
     3061 tctgcctggt ttcggggaag gtgcggctgc cgctgccggg tatgctgggg ttgccgccgg
     3121 gcacgggggt agtggcaggt gtcaccacgg gcgtgttggt ggtggggggc tggcccggcc
     3181 ccacgaaccg cttgagcgtg ccgggcgaat agccgccccc ctccatgaag tagagcgcgc
     3241 cgtccggccc cgtctctatg tccatgttta catcgacgcc ctgcacgatg ttggtgcggg
     3301 tgacgtcagt gcggtcctcg ttcaggtaga agtggcgcag cttgccgccc tggtaagccg
     3361 ccatgaaaag gtggttccgc cactccggta tctgccagcc gtcgtacact tcgatgccgg
     3421 tgggcgcttc gcagcaggcc gagcgggcct ggtaccagag gggtgggatc gtgttcacgt
     3481 cgtcgggcgg gttggcgtcg tcgcaggggt agttggcgcg ccagccgtag ttgaagccgc
     3541 gctcgatgcg gttcatctcg tcgtcgcagc cggggccgtt ttcgctggcg aagatgcgcc
     3601 cccgcaccac cgggtcgaag gtgaagtcga agctgttgcg caggccgtag gcgtagatcg
     3661 aaccgaccgc gcccggcacg ttccagctcg ggttgtcggc ggggatagag ccgtcggggt
     3721 tgaggcggtg gattttgccg ttcttggtgg tcaggtcctg ggcgttgtcg gcgttggcgt
     3781 tgtcgcccac ggagacgtag agcatgccgt ccggcccgaa gtggaggttg ccgccgtcgt
     3841 ggttgcccgc cgtctgtgga gaggcgaaga taacggtcgg gttggagcca acgccgtcgc
     3901 gctccacgaa gcgcactacc ctgttctcgg tgttgctgcc gggggccgtg tgatagatgt
     3961 atatgaagcg gttctggttg aaggtggggt caatcgcgat acccaacagg ccgcgctccg
     4021 agttggtgct cacgtcgaag tgtatcaccg ggttgggctg tagcttgccg cccgcgaaca
     4081 ggcgcacgtt acccgtgacc ttctcggtgt agaagaggcg gcccgccggg tcgaaggcca
     4141 tcgcaatcgg gcggtccatg tcggagagca ccgtctccac gcggacgccc ggcggcagcc
     4201 cctccgtgcc gtcgtcctgg gcggcgggcg cgctcagggc agcggagcct ggcagtaccg
     4261 ccagcatggt ggcggcaacg agcacaaggg tagaagcgat acgcgtgagt ggctttcggc
     4321 tgatgcgcat tatgtccata tcctttcgtg ggagtgggcc gcagt
//
LOCUS       DASRWZ010000317         1896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_712135, whole genome shotgun sequence.
ACCESSION   DASRWZ010000317 DASRWZ010000000
VERSION     DASRWZ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712135"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VFH60_04915"
     CDS             <1..773
                     /locus_tag="VFH60_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313155.1"
                     /translation="YNRVWPRNDTFLAFQQTGASMNYSHLEIAHMQAEALFVHDARDR
                     LLRVNEPDPDGPAPRFFLVRTTLGNLWRARYDLPADLSAELEALVASEPVAGDLGELR
                     EPPYHLGEYTELLKQHAPLSSTDAGPAYYLPELPPPTGSVTITPANVDLLGAHYPYTR
                     SRYVELAPVVVRVVDGVAVAVCCSARLTKQVPEAGVHTVEAYRGRGFALDVVRGWAAD
                     IRATGRLPLYSTSWENMASQAVAGKLGAVLYGADVSIT"
     gene            complement(804..1676)
                     /locus_tag="VFH60_04920"
     CDS             complement(804..1676)
                     /locus_tag="VFH60_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6313156.1"
                     /translation="MFEGFTLSMIDTGEATIRVRHGGSGPPLLLLHGHPQTHVMWYKL
                     APILARDFTVVAADLRGYGDSSKPPTTPDHAPYSKRAMSRDQVEVMRQLGFERFFLAG
                     HDRGGRCAYRLALDHPERVLKLAVLDIVPTGEAWRRANMEFSLGFWHWAFMAQPYDFP
                     ERLIGADPDYFYWRRHAPEPPPFIAPDAFEDFRRSFTNPDTIHTMCEDYRAGATIDFE
                     HDMADRGKKRIACPVLALWSGRDELDRWYDVLSVWREWADDVRGRALDCGHHLALEAP
                     DETYAELHAFFTGG"
     gene            complement(1699..>1896)
                     /locus_tag="VFH60_04925"
     CDS             complement(1699..>1896)
                     /locus_tag="VFH60_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313157.1"
                     /translation="LTLALLPFLRYSLAWYLPGLIAGTLLLAAGVTVYLRDASKVRVM
                     SLLLEMGAMSIAVGWVAAITL"
BASE COUNT          343 a          572 c          647 g          334 t
ORIGIN      
        1 catataatcg ggtatggccg cgaaacgaca catttctagc cttccagcaa acgggtgcga
       61 gcatgaacta cagccatctt gaaatcgccc atatgcaggc cgaggctctg tttgtgcatg
      121 atgcacgcga caggttgttg cgcgttaacg agccggaccc ggatggacct gcccctcgct
      181 tcttcttggt ccgcacgacg ttaggcaacc tctggcgcgc tcgctacgac ttgcccgcgg
      241 acttgagcgc agagcttgag gcgcttgtcg ccagtgagcc ggttgccggt gacctagggg
      301 agctacgcga gccgccttat cacctgggcg aatatacgga gctactcaag cagcacgcac
      361 cactcagcag caccgatgca ggccctgctt actacctgcc ggaactcccg ccgcctacgg
      421 gcagtgtgac aatcactccc gcgaacgtgg acttgctggg agcgcattac ccatacacgc
      481 ggagcaggta cgtcgagctt gccccggtcg tggtgcgggt tgtggatggg gtggctgtgg
      541 cggtttgctg ctcggcgcgg ctcacgaagc aggtgccgga agcgggggtg cacacggtgg
      601 aggcttaccg cgggcgcggc tttgcgctgg atgtagtgcg agggtgggca gcagatatac
      661 gtgcgaccgg caggctgcct ctgtacagca cgtcgtggga gaacatggcg tcacaggctg
      721 tggcagggaa gcttggggcg gtcctgtatg gcgcggatgt cagcattacc tgaaatgtgc
      781 ggcacaacgt tcgcacgtac ttcctagcct cctgtaaaga aggcgtgtag ttccgcgtaa
      841 gtctcatcgg gcgcttccag ggccaggtgg tggccgcagt cgagggcgcg gcctcgcacg
      901 tcgtccgccc actctcgcca gacgctcagc acgtcatacc acctgtcaag ctcgtccctg
      961 ccgctccaca gggcgagaac ggggcacgct atgcgcttct tgccacggtc ggccatatcg
     1021 tgctcgaaat ctatggtggc cccggcgcgg tagtcctcgc acatggtgtg gatggtatcg
     1081 gggttggtga aggagcggcg gaagtcctcg aaggcgtcgg gggcgatgaa ggggggaggc
     1141 tcgggggcgt gtcgccgcca gtagaagtaa tcgggatcgg ccccaatcag gcgctcgggg
     1201 aagtcgtaag gctgggccat gaaggcccag tgccagaagc cgaggctgaa ctccatgttg
     1261 gcgcgccgcc aggcctcgcc ggtcggtacg atgtcgagca cggccagctt gagcacgcgt
     1321 tcggggtggt cgagggccag gcggtaggcg caacgtccgc cccggtcgtg cccggccagg
     1381 aagaagcgct cgaagccaag ctgccgcatc acctccacct ggtcccgcga catggctcgc
     1441 ttggagtagg gggcgtggtc cggggtcgtt ggtggctttg agctatcgcc gtagccccgc
     1501 aagtcggcgg cgactaccgt gaagtcgcgc gccaggatgg gagcgagctt gtaccacatg
     1561 acgtgcgtct gtgggtggcc gtgaaggagc aggagcggag ggccgctgcc gccgtgccgc
     1621 acgcgaatgg ttgcctcgcc cgtatcgatc atcgacagtg taaacccctc gaacatccgg
     1681 cctcctcgca tcatgttact acagggtaat agccgccacc cagcctaccg cgatagacat
     1741 agcccccatc tcaagcagca gcgacatcac gcgcaccttt gaggcgtcgc gcaggtagac
     1801 cgttacgccc gccgcgagga gcagggtgcc cgcgataagg cccggcaggt accatgccag
     1861 gctgtagcgc aggaacggca gcagggccag cgtcaa
//
LOCUS       DASRWZ010000318         4277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1192117, whole genome shotgun sequence.
ACCESSION   DASRWZ010000318 DASRWZ010000000
VERSION     DASRWZ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192117"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1364
                     /gene="queG"
                     /locus_tag="VFH60_04930"
     CDS             147..1364
                     /gene="queG"
                     /locus_tag="VFH60_04930"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET6313158.1"
                     /translation="MKVAHHRTNSVVADWTPSQAGALKEGIVSHAHGLGFDLVRFTGP
                     EPFPEAQLAMEERIEAGLYSGLSWFNLERAAVAGDPRNLMPGVQTIVSLGISYLSDSP
                     IQPSAPGEPRGRVARYAWGLDYHEVFKDKLWALHAYVQEQLGRDVEARALVDTARIVD
                     RAVAQRAGLGWYGKNTNLLSRERGSWVLLGELLLDVALPPDEPVKTTCGACTRCLPSC
                     PTGALIAPGVLDNNRCISYLTIEHKGCIPREMRPLMGEWVFGCDICQDVCPVNRKATP
                     ANHLEFSPDQGIGSSPSLIELMEMGEEEFRARYRHSPVKRAKLQGMQRNAAIALGNIG
                     DPSAVTILTNALNGPYELVRGHAAWALGRIGGEPAQAALAARLREEESAWVREEIQEA
                     LAEIAQDDASRRS"
     gene            1418..2641
                     /gene="tgt"
                     /locus_tag="VFH60_04935"
     CDS             1418..2641
                     /gene="tgt"
                     /locus_tag="VFH60_04935"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET6313159.1"
                     /translation="MSEQTGNKEFSYEVEKSLDTPVGVARAGRLLTPHGEIATPVFMP
                     VGTQASVKSISPDELKGLNAQIILSNTYHLYLRPGAELIAEFGGLHGFMGWDRPVLTD
                     SGGFQVFSLSHNNKIDDEGVTFKSHLDGSTHRFTPEMVMRVEELLGADIIMVLDECTP
                     YPSSHDYNRKALHRTHSWAERCLRAHSRPDQALFGIVQGSTYLDLRKESARTLSGLDF
                     PGYAVGGLSVGEPKEEMHAMLEETVPLLPSYKPRYLMGVGSPEDLVECVARGIDMFDC
                     VLPTRVARNGALLTKDGRLAIKSPRYSHLQAPIEPDCDCYTCGNFTLGYLHHLFRAKE
                     LLAYRLNSIHNLRFLTRMAEDMRAAILDGTFNDYREEFLARYQTSDRELAVKQRELWL
                     QSHGRAITRGAKAGG"
     gene            complement(2758..4215)
                     /gene="selA"
                     /locus_tag="VFH60_04940"
     CDS             complement(2758..4215)
                     /gene="selA"
                     /locus_tag="VFH60_04940"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HET6313160.1"
                     /translation="MRAEEDGQSTGSYESPSNTGVSAMRGLPSVDKVLAHPALQAARR
                     ELPHDLVTLAARQALNYLRTSLSFGSGAAISLDDIADNAVSLAHLMSSPSLRPAINAT
                     GVVIHTNLGRAPLSKAAVEAITNVARYSNLEYDLEEGQRGSRYVHAVQVLRRVTGCED
                     ALVVNNNAAALVLVLAALGQGREVIVSRGQLVEIGGGFRIPDIMRESGAQLVEVGTTN
                     RTYVSDYEAAITERTALLLRVHASNFRLVGFTTSPGIEEIAELAHAHGLLAVDDVGSG
                     ALLDTTRYGLSPEPRVQTSLQAGADLVLFSGDKLLGGPQCGIIAGKGEVVARLRKHPL
                     ARALRVDKLTLGALEATLLHYLKGEAEREVPVWRMIAATPAQLHARASAWAGELTRLG
                     LACGVEEGQSTVGGGSLPGDTLPTVLLALTPQPGSGSPSPARQAGEWAARLRQSPTPV
                     IARVERGLLLVDPRTVAQDEERTLLDALLSTADAE"
BASE COUNT          806 a         1318 c         1369 g          784 t
ORIGIN      
        1 ccgcctgatc gactctctct ccggtgcctg ccgccgcctt attggcagcg atgacagcga
       61 tatcatcgat gactggctgg cgcaagcccg ctccggcctc aacgagacag aacctatcct
      121 aacggaagct agaaccagtc cccactttga aggtagccca ccacaggaca aatagcgttg
      181 tagcggattg gaccccttcc caggccggcg cgctgaagga ggggatcgtc tctcatgcgc
      241 acggcctcgg cttcgatctc gtgcgattca ctggtcctga gcctttccca gaggctcaac
      301 ttgctatgga ggagcgcatc gaggcgggcc tctattcggg cctgtcctgg ttcaacctgg
      361 aacgcgccgc tgtggccggg gacccgcgta acctgatgcc cggcgtgcag acgattgtct
      421 cgcttggcat aagctacctg agcgactccc ccatccaacc ttccgctcca ggcgaaccga
      481 gaggccgcgt tgcccgctac gcctggggcc tggactacca cgaggtgttc aaggacaagc
      541 tgtgggcgct ccacgcgtac gtacaggagc agcttggcag ggacgtggag gcgcgcgccc
      601 tggtggacac cgcgcgcatc gtggaccgcg cggtggcgca gcgggcgggg ctggggtggt
      661 acggcaagaa caccaacctg ctcagccggg agcgcggttc gtgggtgctg ctcggcgaat
      721 tgctgctcga tgtagctctg ccgcccgacg agcctgtaaa gaccacctgc ggggcgtgca
      781 cgcgctgcct gccttcgtgc ccgaccggcg cgctaatcgc accgggcgtc ctcgacaaca
      841 accgctgcat ctcctatctg acgatcgagc acaagggctg tataccgcgc gagatgaggc
      901 ccctcatggg cgagtgggtg ttcggctgcg acatctgcca ggacgtatgc ccggtgaacc
      961 gcaaagccac gcccgctaat cacctggagt tctcgcccga ccagggtata gggtccagtc
     1021 cgtctctgat agagctaatg gagatgggtg aagaggagtt tcgcgcccgc tatcgccaca
     1081 gtcctgtaaa gcgagccaag ctacagggta tgcagcgcaa cgcagctatc gccctgggca
     1141 atattggcga cccgtccgct gtcaccatac tgacgaacgc tcttaacggc ccttacgaac
     1201 tggtgcgagg gcacgccgcc tgggccttag ggcgcatcgg aggtgagcct gcacaggccg
     1261 ccctggctgc gcgcttgcgg gaagaagaga gcgcgtgggt gcgggaggag atacaggagg
     1321 ccctggcaga gatagctcaa gacgacgcat cgaggcgaag ttaactggct gagagttgca
     1381 ccagatgaca aatacgcgct agaaatggaa caaaacgatg tcagagcaga ccggcaacaa
     1441 agaattcagc tatgaagtag aaaagtcgct cgatacgccc gtgggcgtgg cgcgggccgg
     1501 gagactgctc acccctcatg gggagattgc cacgccagtg ttcatgcccg tgggcacgca
     1561 ggcgagcgtg aaatcgatct cgccggacga gctgaagggt ctaaacgcac aaatcatcct
     1621 ctctaacacg taccacctgt acttgcggcc gggcgcggag ctgatcgcgg agttcggcgg
     1681 cttgcacggc ttcatgggct gggaccgtcc tgtattgacc gacagcggcg gcttccaggt
     1741 gttcagcctg agccacaaca acaagattga cgacgaaggg gtaacattca agtcccacct
     1801 ggacggctcg acgcatcgct ttaccccgga gatggtaatg cgcgtggagg agctgcttgg
     1861 ggccgacatc ataatggtgc tggacgagtg cacgccttac ccctcctcgc acgactataa
     1921 ccgcaaagcg ttgcaccgca cccattcgtg ggccgaacgc tgcctgcggg cgcattcgcg
     1981 gccagaccag gccctgttcg gcatcgtgca gggcagcact tacctggacc tgcgaaagga
     2041 aagcgcccgc acgctatcgg gactcgattt tccgggctac gccgtggggg gcttgagcgt
     2101 aggagagcct aaggaagaga tgcacgcgat gctggaggag acggtgcccc tcttgccatc
     2161 atacaagcca cgctacctga tgggtgtggg gtcgcccgag gacctggtag agtgcgtggc
     2221 gcgaggcatc gacatgttcg actgcgtgct ccctactcgc gtggcgcgca acggggcgct
     2281 actgaccaaa gacggtcgcc tggcaatcaa aagcccccgg tactcacatc tgcaagcacc
     2341 aatcgagccg gattgtgact gttacacatg cggcaacttc actcttggct acttgcatca
     2401 tttgttcagg gcgaaagaat tgctagctta taggctaaat agcatacata atcttcgctt
     2461 tctgacacgg atggctgagg atatgcgggc cgctattctg gacggcacct tcaacgatta
     2521 ccgtgaggag ttcctggctc gctaccagac cagcgaccgc gagctggcgg tgaagcaacg
     2581 cgaactctgg ttgcagtctc acggccgcgc catcacaagg ggtgccaagg ctggagggtg
     2641 agtggtggcc tttgctgccg agacacgcca ggctgctctg ggttttccat aaccctgtaa
     2701 ctcaatctgc aagagagcgc atcaacctta gtattagtgc acgctctcag ttcgcgtcta
     2761 ctcagcgtct gcagtgctca gcagcgcgtc caacagggtt cgctcttcgt cctgcgcgac
     2821 cgtgcgaggg tcaaccagca gcaggccccg ttcgacgcgg gcgattacag gtgtgggtga
     2881 ctgccttagc cgggcggccc actcaccggc ctgacgtgcc gggctggggg agccgcttcc
     2941 gggctgcggg gtgagagcca ggaggaccgt gggaagggtg tcgccgggaa gggagccgcc
     3001 gcccaccgtc gattgtcctt cctccacccc gcacgccagg ccaagccgtg taagctcccc
     3061 ggcccatgcg ctcgcgcggg catgaagctg cgccggggtc gcggcgatca tgcgccaaac
     3121 cggtacctcc cgctcggcct cgcccttcag gtagtgcagc aaagtggcct caagcgcccc
     3181 caacgtgagc ttgtccaccc gcagcgcgcg ggcgagtggg tgcttgcgga gccgggcaac
     3241 cacttctccc ttaccggcga tgatgccgca ctgcgggcct cccagcagct tgtcaccgct
     3301 gaacagcacg aggtcggccc ctgcctgcag gctggtttgt acgcggggtt ccggggaaag
     3361 tccgtagcgc gtggtgtcca gcagggcacc gctacctacg tcgtccacgg cgagcaggcc
     3421 gtgtgcgtgg gcaagctcgg ctatctcctc gataccgggg gaggtggtga agcccaccag
     3481 gcggaagttg ctggcgtgga cccggagcag gagggctgtg cgctcggtga ttgcagcctc
     3541 ataatcggac acgtaggtcc ggtttgtggt gcctacttcc accagttgag cgccgctttc
     3601 cctcatgata tcgggtatgc ggaagccccc gccgatctct acgagttggc ccctggagac
     3661 aattacctcc cgcccttgcc ccagcgccgc aagcacgagc acgagggcgg ccgcgttgtt
     3721 gttcactacg agtgcgtcct cgcagcccgt aacccggcgc aagacctgca ccgcgtgcac
     3781 gtagcgcgag cctctttgtc cttcctcaag gtcgtattcc aggttgctgt accgggccac
     3841 gttggttatc gcctccaccg ccgccttgct tagaggtgcg cggccgaggt tggtatggat
     3901 gaccacgccg gtggcgttaa tcgcggggcg cagactgggc gacgacatga ggtgagcgag
     3961 agagacggcg ttgtcggcta tgtcgtcgag cgaaatggcc gcaccgctgc cgaacgacag
     4021 ggacgtgcgc aggtagttga gtgcctggcg ggcggccagc gtcacaagat cgtgtggcaa
     4081 ttccctcctg gcggcctgca atgccgggtg ggcaagcacc ttgtccaccg aaggtagccc
     4141 gcgcatcgcg gaaacgcccg tgttcgatgg ggattcgtag ctgcccgtac tttgtccgtc
     4201 ctcttcggct ctcatggtga tattgtacca cggcaaaaaa agaaggggac ggctgtgagg
     4261 ccgtcccctt ctgcttt
//
LOCUS       DASRWZ010000319         7054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_575690, whole genome shotgun sequence.
ACCESSION   DASRWZ010000319 DASRWZ010000000
VERSION     DASRWZ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575690"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..2263
                     /locus_tag="VFH60_04945"
     CDS             59..2263
                     /locus_tag="VFH60_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6313161.1"
                     /translation="MTTLIGASVPRPDALGKVTGATRYPADLVRADMLHLKIVFAHRP
                     HALILKLDTSKALEYPGVVDVLTAADVPFNAYGLVESDQPALCSDKVRYVGDRVAVVV
                     AQTVEAAEAGAALVEVEYEDLPPVVDARAAMSPDSPLVHEAQGTNVLGHVPIRKGDAD
                     KALDEADVVLEGEFTTSWQEHAYLQPEAGIAYIDQAGKLVIETAGQWLHEDRRQIAEM
                     LKLPEDDVIIRYAAIGGAFGGREDLSIQHLLALATWKLKRPTAMIWSREESMFAHHKR
                     HPIAIRCRWGAMRDGRIVGVRAELVADGGAYASTSVEVLKIAALFATGTYEVPNISVD
                     GYAVYTNNVPCGAFRGFGAPQAQFAVESMVTRLAHALGIDPIEMRRRNIYREGSIEPS
                     QGPLPPGVSALPVMERCVEEAKARLDYGSPRPQPKKPHLRRGVGIACGIKNIGYSFGY
                     PDQATAEVELYGSDKIEHATVRVGAADVGQGAHTILRQIAAETLGLPFEHPQGAGESP
                     LIEMVTDDSAQAPNAGSASASRMTYMGGRAVHDASKAALAKWESGERPARARVQFRPA
                     PTTGLDPETTAGEPNFAYGYAAQAVEVEVDTLSGLVQVLKIISVHDVGRAVNRQQVEG
                     QIEGCLAQALGYALLEDFQMRDGKVLTPYFSTYLLPTALDMPTEIVPVIMELADPNGP
                     YGARGMAEMPLVPLTPAIAIAIHDATGVWLSQQPMTPERVLVALSEGEPGKG"
     gene            complement(2479..3099)
                     /locus_tag="VFH60_04950"
     CDS             complement(2479..3099)
                     /locus_tag="VFH60_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313162.1"
                     /translation="MSNTALWDNSDCAAWHQALDSYSQVVRAQDASGLEELDLWYREE
                     LPGLLASRTPPYATRDELVRATRWKMKRGVWRQRNLLLVEGNSESDVEQTSREALAAV
                     PDPRKPVSLLVRLAGVGPATASAVLSAHSPQVYPFFDELVAEQIPGLGDVAFTAPYYA
                     RYAELLRTRAQQLSGECSHRAWTANDVSQALWAASGGKAARVPTVP"
     gene            3098..3238
                     /locus_tag="VFH60_04955"
     CDS             3098..3238
                     /locus_tag="VFH60_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313163.1"
                     /translation="MQAAPTFEVRVGGANIERGGAVVNGGGGFAIVGEALRERPVGVP
                     CH"
     gene            3566..4792
                     /locus_tag="VFH60_04960"
     CDS             3566..4792
                     /locus_tag="VFH60_04960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313164.1"
                     /translation="MRILFVTPRFPYPPLKGDSLRSYHQIRALSRRHQVSLLSMSTSP
                     VGEAERAHMLELCESVTVVPLARGQVWLNLLAGLPSRIPLQVSYFRSAEFRHALDNLL
                     ADGRFDLVHATLIRVLPYVWDLRHTPVAVDLIDSLTLNLQDRRVKVRWPLRLGYEIEY
                     SRVRRYEAEVVRHFPGLAVSSPADRQVLGSDSVAVLPNGVDLEGFPFYAPGDRDPSTV
                     VFTGNMGYHPNEEAVAWFAGEVWPLVRSARPEARFQVVGTNPGERVRALSGSHGIEVV
                     GKVPDVAAYLGIAAVSVAPMNTGSGIQNKVLEAMSTATPVVATSTANRGVGGIPGRDL
                     LVADEPGELAAAVLRLMDDAHLRAQLGKAGRDFVERQFRWEGHAEGLERLYEQVLKSH
                     DRVANAGQALALSVEG"
     gene            4797..5939
                     /locus_tag="VFH60_04965"
     CDS             4797..5939
                     /locus_tag="VFH60_04965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313165.1"
                     /translation="MPEKLNVVQMTDVTGRGGAEKALVDLALRLDRTRFNVTVVATRA
                     TGNYQPLLDAAGVRTLVIARDSRWQSYKLLNLARFMRRQPVHILHTHLFGANTWGRVL
                     GKLARVPVIVAHEHWSSKAPREVWVDRLLYRLTDRILVPSHASKQLVMEMEGIPARYL
                     DVTYNGVDISRFSQTAESASVRQELGLGPEDFVVGTVGRLSADKGGQDDLLWAASELR
                     KSHPRARLLIIGDGPLRPDLEKYAAELGMLDAQAAIFTGTRNDVDRLLGAMDVFVLPS
                     HMEALPIAVLEAMSMCLPVVATRVGGVPEVVQDGSTGLLVEPGDRPGMRDALARLASD
                     RSLAHCLGTSGQARVRSNFTVDKMVRHVEELYEELFDKKASKKRKR"
     gene            6289..6939
                     /locus_tag="VFH60_04970"
     CDS             6289..6939
                     /locus_tag="VFH60_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313166.1"
                     /translation="MEVGTYLRIIARYWWVVLLSMLAAGGGAALLDAVRAPRYSVQAR
                     VAARPAAVLSNTVTIVDLVGQLGSRHVTGTLAQTFTSADVKRAAREAAGLSFEDAQAY
                     DLEANVLPDSSVIAISASGPDPNALALYVNSTITNTVSKAHDYYSVIDLEPLEMANPP
                     TEPSSPVPARDIPLGVALGLALGVLLAIALDYLVGSRGVQSELRALHPYYNQEVSR"
BASE COUNT         1284 a         2295 c         2296 g         1179 t
ORIGIN      
        1 gtgtggctgt ggtacgaact cgtcggcagc aagagcgagg aagtaggaag gtgagcagat
       61 gacgaccctc ataggggcct ccgtgccccg ccccgacgcg cttggcaaag tcaccggcgc
      121 gacccgctac cccgccgacc tcgtgcgggc cgatatgctg cacctcaaga tcgtgttcgc
      181 ccaccgcccc cacgccctca tcctcaagct ggatacgagt aaggccctgg aataccccgg
      241 cgtggtggac gtgctgaccg ccgctgacgt acccttcaac gcgtacggcc tggtggagtc
      301 ggaccagccc gccctttgca gcgacaaggt ccgctacgtg ggcgacaggg tggctgtggt
      361 ggtggcccaa actgttgagg ctgccgaggc gggggccgcg ctggtggagg tagagtacga
      421 ggacctgccg cccgtggtgg acgcgagggc cgctatgtcg ccggattcgc cgctggtgca
      481 cgaggcgcag ggcaccaacg tgctggggca cgtgccgata cgcaagggag atgcggacaa
      541 ggcgctggac gaggctgatg tggtgctaga gggtgagttc actacctcct ggcaggagca
      601 cgcctatttg cagccggagg cgggtatcgc ctacatcgac caggcgggca agctggtgat
      661 agagacggcg ggccagtggc tgcacgagga ccgccgccag attgccgaga tgctgaagct
      721 gccggaagac gacgttatta tccggtacgc ggccattggc ggggcgttcg gcgggcggga
      781 agacctgtct atacagcacc tgctggcgct cgccacctgg aagctgaagc ggcccaccgc
      841 gatgatatgg agccgcgagg agtcgatgtt cgcccaccac aagcggcacc ctatcgccat
      901 acgctgccgg tggggcgcga tgagggacgg gcgcatagtg ggtgtgcggg ccgagctggt
      961 ggccgacggc ggggcctacg cctccaccag cgtggaagtg ctcaagatag cggcgctgtt
     1021 cgccacgggc acatacgagg tgcccaacat ctcggtggac ggctacgcgg tgtacaccaa
     1081 caacgtgccg tgtggggcgt tccgtggctt cggggcacca caggcccagt tcgcggtaga
     1141 gagcatggtc acgcggctgg cccacgcgct cggtatcgac ccgatagaga tgcgtcgccg
     1201 caacatctac cgggagggca gcatcgagcc gtcgcaaggt ccgttgccgc cgggggtgag
     1261 cgcccttccc gtgatggagc ggtgtgtgga ggaggcgaag gcgaggctcg attacggcag
     1321 cccgcgcccg cagccgaaga agccgcacct gcgccggggc gtgggcatcg cgtgcgggat
     1381 aaagaacatc ggctactcgt tcggctaccc cgaccaggcg acggccgagg tggagcttta
     1441 cggcagcgac aagatagagc acgccacggt gcgggtgggc gcggcggacg tggggcaggg
     1501 ggcgcacacg attttgaggc agatagcggc cgagacgctg ggcctgccgt tcgagcaccc
     1561 ccagggcgcg ggcgaatccc ccctgattga gatggtgacc gacgatagcg cgcaggcccc
     1621 caacgcgggc agtgcctcag cctcgcgcat gacctacatg ggcgggcggg cggtgcacga
     1681 cgcgtcgaag gcggccctcg cgaagtggga gagcggggag cgtccggccc gcgcgcgagt
     1741 gcagttccgc ccggctccaa ccacggggct ggaccccgag accacggcgg gcgagccgaa
     1801 ttttgcctac gggtacgcgg cgcaggcggt ggaggtggag gtcgatacgc tgagcgggct
     1861 ggtgcaggtg ctcaagataa tcagcgtgca cgacgtgggc agggcggtca accgccagca
     1921 agtggagggg cagattgagg ggtgtttggc gcaggcgctc ggctacgcgc tgttggagga
     1981 cttccagatg cgcgacggca aggtgctcac gccctatttc agcacctacc tgctgcccac
     2041 cgccctcgac atgcccaccg agatcgtgcc cgtcatcatg gagctggccg accccaacgg
     2101 cccttacggg gcgcgtggta tggccgagat gcccctggtg cccctcacac ccgcgatagc
     2161 catcgccatc cacgacgcga cgggggtatg gctcagccag cagccgatga cgccggagcg
     2221 ggtgctcgtc gcgctaagcg agggtgagcc tggcaagggg tgaaaagtgg agttgaaacg
     2281 ttaggcagcg agatgctaaa cgccaagacg ccaagacgcc caggaccacg ccaagattaa
     2341 ggatggggac tcaagataaa ggagcagggt acgaaaccat gtaccctgct cctctttgta
     2401 ttctcctccg gggtaattac ccacgttgta ggcccggtac tcgcgcacct ggggcgggta
     2461 cgcgacgcga gttggagatt atgggaccgt gggcacacgc gcggcttttc cgccggaagc
     2521 cgcccacagc gcctggctta catcgttcgc tgtccaggcg cggtgcgagc actccccgct
     2581 cagttgctgt gcgcgggtgc gtagcagttc ggcgtagcgt gcgtagtaag gggcggtgaa
     2641 ggccacgtcg cccaggccgg gaatctgctc ggccaccagc tcatcgaaga aggggtagac
     2701 ctgggggctg tgggcggaga gcaccgccga ggctgtggcc gggcctacgc ccgcaagccg
     2761 gactaacagg ctcaccggtt tgcgggggtc gggcacggcg gccagggcct cacgggaggt
     2821 ctgctctaca tcgctttccg aattgccttc caccagcagc aggttgcgct gccgccacac
     2881 gcccctcttc atcttccagc gcgtggcccg cacaagctcg tcgcgggtcg cgtagggagg
     2941 cgtgcgcgag gcgagcaagc ccggcaattc ctcgcggtac cagaggtcga gttcctcaag
     3001 gccgctcgcg tcctgcgccc gcacgacctg gctgtaacta tcgagggcct ggtgccaggc
     3061 ggcgcagtcg gagttgtccc atagagcggt gttgctcatg caagctgctc ctacgttcga
     3121 ggtaagggtt ggtggggcca atatagagcg tggcggggcg gttgtcaacg ggggtggggg
     3181 cttcgcgata gtgggggagg ccctgcggga acgccctgtg ggtgtgccct gccactaaag
     3241 tggcgggctg accagagacg aagtctcctt cggagactgc cctgatgttc cttagtcccc
     3301 gaaggggact ttgcctcttc tcagcccgtg gctttagcca cagggcgtcc ccgcagggcg
     3361 ttcatgccgg gccgacgcac ctactcacca atcctctact tctttcggta acatccgggc
     3421 cacacagacg gtactatagt ccccgccgct ttatcccatc gggtaatagc caacccagtc
     3481 cgggtagttt atcatgttat agacactact ggccccgcgc ccgcacccgg ccattactcg
     3541 tcacgaggag cttcataccg gcaatatgcg catccttttc gtaacgccac gcttcccata
     3601 cccgcccctc aaaggcgaca gcctgcgctc ctaccaccag atacgcgccc tcagcaggcg
     3661 gcaccaggtc tccctcctgt cgatgtcaac gtctccggtg ggtgaggccg agcgcgccca
     3721 catgctggag ctttgcgaga gcgtcaccgt cgtccccctc gctcgcggcc aggtgtggtt
     3781 gaacctcctg gcgggcctcc cctctcgcat tcccctccag gtgagctatt tccgctccgc
     3841 cgagttccgc cacgccctcg acaatctgct cgcggacggc cggttcgacc tcgtgcacgc
     3901 caccctcata agggtgctgc cctacgtatg ggacctccgg cacacgcccg tcgcggtgga
     3961 cctgatagac tccctcaccc tgaacttgca ggacaggcga gttaaagtac gctggccgtt
     4021 acgcctgggc tacgagatcg agtactcccg cgtgcgccgc tacgaggccg aggtggtgcg
     4081 gcatttccca ggactggcgg tgtcctctcc cgccgaccgg caggtgctgg gcagcgacag
     4141 cgtggccgtg ctccccaacg gcgtggacct ggaaggcttc cccttctacg cccccggcga
     4201 ccgcgacccc tcaacagtgg ttttcaccgg caacatgggc taccacccaa acgaagaagc
     4261 cgtagcctgg tttgccggcg aagtgtggcc cctcgtccgc tccgcccgcc ccgaggcccg
     4321 ctttcaggta gtcggcacca accccggcga gagggtgcgc gccctttccg gcagccacgg
     4381 cattgaagta gtcggcaagg tgcccgacgt ggcggcgtac ctgggcatcg cggccgtttc
     4441 ggtagccccc atgaacaccg gctccggaat ccagaacaag gtgctcgaag ccatgtccac
     4501 cgccacgccc gtagttgcca cgtccaccgc caaccggggc gtaggtggca tccctggccg
     4561 cgacctgctg gttgcggacg agccgggaga actggccgct gcggtgctcc gcctgatgga
     4621 cgacgcccac ctccgggcgc aacttggcaa agcgggccgc gacttcgtgg agcgacagtt
     4681 ccgctgggag ggccacgccg agggcctgga acgcctctac gagcaggtgc tgaagtccca
     4741 tgatagagta gcaaatgccg ggcaggcgct cgcgctctca gtagaaggat aagggcttgc
     4801 cagagaaact gaacgtggta cagatgaccg acgtaacggg ccggggcggt gccgaaaaag
     4861 ccctggttga ccttgccctg cgcctcgacc gcacccgctt caacgtcacc gtggtagcca
     4921 cccgcgccac cggcaactac cagcccctac tcgatgccgc cggggtgcgc accctggtaa
     4981 tcgcgcgcga tagccgctgg cagagctata agctgctcaa tctcgcccgc ttcatgcgcc
     5041 gccagcccgt gcacatcctc cacacccacc tcttcggcgc gaacacctgg ggccgagtgc
     5101 tgggcaagct ggcgcgggtg cccgtaatcg tcgcgcacga gcactggtcg tccaaggccc
     5161 ctcgcgaggt gtgggtggac cgccttctct accgcctcac cgaccgcata ctcgtgccct
     5221 cccacgccag caagcagctc gtcatggaga tggagggaat ccccgcgcgc tacctcgacg
     5281 tgacctataa tggtgtggac atatcgcggt tcagccaaac ggccgagtcc gcatcagtgc
     5341 ggcaggagtt ggggttgggg ccggaggact tcgtagtggg tacggtgggc cgcctgagcg
     5401 ccgacaaagg gggccaggac gacctgctct gggccgcgag cgagctaagg aagtcgcacc
     5461 ctcgcgcccg gctgcttata ataggtgatg ggccgctacg tcccgatctg gagaagtacg
     5521 ccgccgagct tggcatgctg gacgctcagg ccgcgatatt caccggcacc cgcaacgacg
     5581 tggaccgcct gctcggcgcg atggacgtgt tcgtcctccc gtcgcacatg gaggcgctac
     5641 cgatagccgt gctggaagcc atgtcgatgt gccttcccgt ggtagccact cgcgtgggtg
     5701 gcgtgcccga agtggtgcag gacggctcta ccggcctcct ggtggagcct ggcgacaggc
     5761 ccggcatgcg ggacgccctt gcccgactcg cctccgaccg cagcctggcc cactgcctcg
     5821 gcacctccgg ccaggcaagg gtccgctcca acttcaccgt ggacaagatg gtgcggcacg
     5881 ttgaggaatt gtacgaggaa ttattcgata agaaggcgag caagaaacgt aaaagatgat
     5941 tccggctgct accggtggta cgtggaggcg agcaggcgct tctagagaac caactcaccc
     6001 ctttgtcatt ctgagcgtag cgaagaatct cgtcctctac cctgatgtta caattttctt
     6061 gagtgcgaat gctggcataa atagagcaga aacttgggtg ctgtaggacg agattcttcg
     6121 ctacgctcag aatgacaaag ggagataggg tacagctaca ggggcagagt cgcattcacg
     6181 tacccgcacc cggaagacgc atcactcgtc actcgtcact cgtcactcgt cactcgtcac
     6241 tcgtcactcg tcactcgtca cttcttatac caatgaggta aaggaactat ggaagtcggt
     6301 acgtacttac gcataatagc ccgctactgg tgggtggtat tgctgtcgat gctggcggcg
     6361 gggggcgggg ccgcgctgct ggatgcggtg cgcgccccca ggtacagcgt gcaggctcgc
     6421 gtggcggccc gcccggcggc ggtgctgtcc aacaccgtca cgatagtgga cctcgtgggg
     6481 cagctaggct cccgccacgt caccggcacc ctggcgcaga cgttcacctc ggctgacgtg
     6541 aagagagccg cgcgagaggc cgccgggctt tcgttcgaag atgcccaggc atacgacctg
     6601 gaagccaacg tcctgcccga ctcctccgtg atcgccatca gcgccagcgg ccccgacccc
     6661 aacgccctcg ccctctacgt caactccacc atcaccaaca cagtgagcaa agcccacgac
     6721 tactacagcg tcatagacct cgaaccgctg gagatggcaa acccacccac cgagcctagc
     6781 tcacccgtcc ccgcccgcga catccccttg ggtgtagccc tcggccttgc cctcggcgtg
     6841 ctcctcgcca tcgccctgga ctacctcgta ggctcaagag gcgtccaaag cgaactccgc
     6901 gccctccacc cctactacaa ccaggaagta agcagataga acgattttgg attttggatt
     6961 agaagcgcaa tccaaaatct aaaatccaaa atccgtcatg tacgctccta ctctcaggcc
     7021 accgcccgcc atactcgacc caggcgtcac cagg
//
LOCUS       DASRWZ010000320         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_575900, whole genome shotgun sequence.
ACCESSION   DASRWZ010000320 DASRWZ010000000
VERSION     DASRWZ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575900"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1115
                     /locus_tag="VFH60_04975"
     CDS             150..1115
                     /locus_tag="VFH60_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313167.1"
                     /translation="MQIKHLSRLVAAIGVAALLVAAGCGDGAGITQPAAPTVTSDMGA
                     EAKIAKPVLAVTDLVVGHNRFVFGLAEETTGQPITDVPQVTVQFFKVHPDGTATKMSD
                     ASVVAHGENLPAFLFVAKADFTEAGDWGALLTIQREGQRSYQTSLDFKVVADSAVPVV
                     GEVPPLSRNLTIKDDPTLDNICSAQPHDDMHNMTIAEAVESGRPSLLLFAAPGFCPSF
                     TCGPNLEIALALQQKYRDKVNFIHIETPNTLQDHAHTLPVEANHRQQPGHYGIPLPDL
                     KTMEEWALQSEPWIFLLDSQGKVASRFEGGLTLDEVEPELARLVK"
     gene            complement(1578..3713)
                     /locus_tag="VFH60_04980"
     CDS             complement(1578..3713)
                     /locus_tag="VFH60_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET6313168.1"
                     /translation="MSRNLSHKGKGLDTGPVALPVGDDLQDVSPAKGNGKSTSRLSLS
                     TPSARRTDQALDDAQSSLDSLIKGPNETSGRSLPQVISSSGRVRNETRGRIAAPASLT
                     RDTMPTSTARTARPTYILGDGMDDHDDRALAVVPGTVPLYGRDGEVREVPNTALGRGV
                     ALATGTLQTARENAGLLLGTLDGPTGLGIADPEALHTSRRAASQWATRYATHLVVLLV
                     VGVLVALGGLKALTTQGAYSSDLHSAEALGTDHFEHAEEDEYGGQEIDSNDFEIALPR
                     TELGGADAAANSIVAPLAPSGDSVQPQPQAQPVTVAIARATEYTVVAGDSISSIAARF
                     KLMPETVMGSNGIYDNEEVLAAGRVLVIPPIDGMYYVAKEGDSVQTIAERFQVDPEMI
                     TSFAGNNLVNGAVAPGQSVIVPGGMMPPRDVAVMYTVRKGDTLKRVAARYGIDVPTVL
                     HANDIADPDNLSIGSELRLLPVPGIEYTVAKGDTLFSIADKLGVSAEMILDYPPNNLR
                     ADSVLQIDAKIMVPGGSPPVVMAARVAPVEKSEARAPQPPAKQQPAAKSANKPAAPVK
                     PAPKPEPKPAPKPPAANTPRKGTGRMVWPVRGTITQYYSGRHNGLDIAVRAGTPIHAA
                     DAGKVIWSGWRTDGLGYCVMIDHGNNLTTIYGHMIKQPPVRVGQYVNQNQVIGYIGST
                     GRSTGPHVHFMVKVGTGKNYRNPLGYLGK"
BASE COUNT          764 a         1216 c         1327 g          737 t
ORIGIN      
        1 gaacgggcgg ctgatccttc gctacgctca ggatgacaaa gggagcaggg tgcgaaaaaa
       61 gcgacagcaa ccttaatcct ggtatagaac catatcgcac ctgccaacca aatcctaaat
      121 cctaaatccc aattccagag agcgtaagca tgcaaatcaa acacctgagc agacttgtag
      181 ccgccatagg cgtagccgct ttactcgtag cggcgggctg cggcgacggc gcgggcatca
      241 cccagcccgc cgcgcccacc gtgacctcgg acatgggagc agaggctaag atcgccaagc
      301 cggtgctcgc ggtgaccgac ctggtggtgg ggcacaatcg cttcgtgttc ggccttgccg
      361 aggagaccac cgggcaaccc ataaccgacg tgccccaggt caccgtgcag ttcttcaaag
      421 tccaccccga cggcacggcc accaagatga gcgacgcaag cgtggtggcc cacggggaaa
      481 acctgcccgc gttcctgttc gtggcgaagg ccgacttcac ggaagcgggc gactggggcg
      541 cgctgctcac gattcagcgc gaagggcaac gctcctacca gacaagcctc gacttcaaag
      601 tggtggccga cagcgccgtg ccggtggtcg gagaggtgcc gcccttgagc cggaacctga
      661 cgatcaagga cgaccccacc ctggacaaca tctgctcggc ccagccccac gacgacatgc
      721 acaacatgac gattgccgag gccgtcgagt caggcaggcc ctctctcctc ctgttcgccg
      781 cgcccggctt ctgcccaagt ttcacctgcg gccctaacct cgaaatcgcc ctggccttgc
      841 agcagaaata ccgcgacaag gtgaacttca tccacatcga gacgcccaac accctccagg
      901 accacgccca caccctgccc gtcgaggcga accaccgcca gcagcccggc cattacggca
      961 tccccctgcc cgacctgaag acgatggagg agtgggcctt gcaaagcgag ccctggatat
     1021 tcctgctaga cagccagggc aaagtcgcca gccgtttcga gggcggcctg accctggacg
     1081 aggtggagcc ggagcttgcc aggctggtca agtgaggcga gttgttagtt gttagctgtt
     1141 agcgaagagg cggaaaccgc aaaggcgcgc aggccgcaaa gaacgcgcta agactaggga
     1201 ctatctgcaa agtgtagaaa cgagcgaaca aaggtggtct ttgtcattct gagcgcagcg
     1261 aagaatctcg tcctccatgc aggtgttgcc cttttcttta gcgcagggct gcgcatgcca
     1321 ccttaccggc ctacagaaaa gtggtacttg ggctacttgg gtgctgaggg acgagattct
     1381 tcgctacgct cagaatgaca aagtgggaga gagtcctgcc tgggcggcac tttgcagata
     1441 agctctgtga aagtttgggg tgggcggcaa gcgctgcttc tcgccacacc aagcaacaag
     1501 aaggctcaag ggacttgctt ctcccttgag ccttcttacg tgttacgagt tacgagttac
     1561 gagttacgct ttactcgtca cttccccaga taccctagcg ggttcctgta gttcttaccc
     1621 gtgccgacct tcaccatgaa gtggacgtgg gggccggtgg agcggccggt ggagccgatg
     1681 tagccgatta cctggttctg gttgacgtac tggcccacgc gcaccggggg ctgcttgatc
     1741 atgtggccgt aaatggtggt aaggttgttc ccgtggtcga tcatcacgca atagccgagg
     1801 ccgtccgtgc gccaacccga ccagatgacc ttgccggcgt cggcggcgtg aatgggagtg
     1861 cccgccctga cagcgatgtc gagtccgttg tgccggccgc tataatactg ggtgatggtc
     1921 ccgcgcacgg gccaaaccat gcgccccgtg cccttcctgg gtgtgttggc cgcgggcggc
     1981 ttgggcgcgg gcttgggttc gggcttgggc gccggcttca cgggcgcggc gggcttgttg
     2041 gcagacttcg ccgcgggctg ctgcttggcg ggaggctgag gggcgcgggc ttcactcttc
     2101 tccacgggcg cgacacgagc cgccataacc accggggggc tgccaccggg caccatgatc
     2161 ttcgcgtcga tttgcagcac gctatcggcc cgcaggttgt tgggcgggta gtcgaggatc
     2221 atctcggcgc tgacgccaag cttgtccgcg atggagaaga gcgtatcgcc cttcgccacg
     2281 gtgtactcta tgccgggcac gggcagcagg cgcaactccg agccgatgga caggttgtcg
     2341 gggtctgcga tgtcgttggc gtgcagcacc gtgggcacgt ctatgccata ccgggcggcc
     2401 acgcgcttga gggtgtcgcc cttgcgcacc gtgtacatca ctgccacgtc gcggggtggc
     2461 atcatgccgc cgggcacgat taccgattgc cctggcgcga ccgccccgtt cacgaggttg
     2521 ttgcccgcga aggaggtaat catttcggga tcgacctgga accgctcggc gatggtctgc
     2581 acgctatcgc cctctttggc gacgtagtac ataccgtcga tgggcggtat cacgagcacg
     2641 cggcccgccg ccagcacttc ctcgttgtcg tagatgccgt tcgaccccat gaccgtttcg
     2701 ggcatcagct tgaagcgggc tgcgatggag ctgatgctat ccccggctac cacggtatac
     2761 tcggtggccc tggcgatggc aaccgtcacg ggctgcgcct gcggctgggg ctgcacgctg
     2821 tcgccggagg gagcgagggg cgcgacgatg gagttcgcgg cggcatcggc cccgccaagt
     2881 tcggtgcggg gcagggctat ctcgaagtcg ttgctatcta tctcctggcc gccgtattca
     2941 tcctcctcgg cgtgctcgaa atggtcggtg ccgagggctt cggcggaatg gaggtctgag
     3001 gagtaagcgc cctgagtggt gagggccttg aggccgccca gggccaccag cacgcccacc
     3061 acgagcaaca ccaccaggtg cgtagcgtag cgggtggccc actgggaagc ggcgcggcgg
     3121 gaagtgtgca gcgcctccgg gtcggcaata cccagccccg tggggccatc gagggtgccc
     3181 agcagcagcc cggcgttttc cctcgcagtc tgcaacgtgc ccgtggcaag ggccacgccc
     3241 cggcccagcg cggtatttgg cacctcgcgg acttcgccgt cccgcccgta caggggcacg
     3301 gtgccgggca cgacagccag cgcgcggtcg tcgtggtcgt ccatgccgtc gcccaggata
     3361 taagtgggac gggcggtcct ggcggttgat gtcggcattg tgtcgcgagt gagagaggcg
     3421 ggagcggcga tgcggcccct ggtctcgttg cggacgcggc ccgaggagga aatcacctgg
     3481 ggcagcgagc ggccggaggt ctcgtttgga cctttgatga gggaatcgag gctgctctgg
     3541 gcatcgtcga gagcttggtc tgtacgcctc gcagaggggg tgctgagtga taggcggctg
     3601 gtggacttcc cgttgccctt ggcgggggaa acgtcctgaa ggtcatctcc gaccggaaga
     3661 gccaccggcc cagtgtccag acccttgccc ttgtgggaca ggtttcgtga catgtagcgg
     3721 tatcctcctg ttatgtaaac agaaaataga ggccgtatag gcgacctcta tgggcggcga
     3781 gcactaaagc gacgagcggc atgcagaatg aagccactca agtacagatt gtctcgcgcc
     3841 cataatgcct cggtaagagg caaagcaccc acacgacgta ggggcaaggc gtatgatacc
     3901 agccgtacaa aagcaatgtc aagaacgacc gggggcgtgg gctacttcta tacagcaaaa
     3961 gtacgcttaa ttacgtagta tgttatgtat agacgaaggg gtagcctgtg cgtgagcggc
     4021 agtttgtgga agagcggtcg ggga
//
LOCUS       DASRWZ010000321         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1123678, whole genome shotgun sequence.
ACCESSION   DASRWZ010000321 DASRWZ010000000
VERSION     DASRWZ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123678"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VFH60_04985"
     CDS             complement(<1..901)
                     /locus_tag="VFH60_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313169.1"
                     /translation="MDSPPPDEESPFRHGLLKSVWQARGFFGGILAIQIAYYGQRALM
                     DRDDYTFSTFCYGLAILVLILSLLHPNLPRRRAAAPTAQATVVDTPEAAAAVDLAAGN
                     GHTPGATSPAAPPEARVSNATPAALPMRKAGSGSASVAATQDSSVEAPGNGTPLATAR
                     SEPEVPGASTDPVGTRKAQPNNGSTPQTVTLVTAESTVARTAWQRIPKAVARLAAWRA
                     REGWRVTVPALLVAVGLLVWSFFALRANIVEPVGGWLWAGGLVALWVALLGVPGWQKG
                     KSLLPGPREEFFGRGVPGLDARWS"
     gene            complement(1042..>3160)
                     /locus_tag="VFH60_04990"
     CDS             complement(1042..>3160)
                     /locus_tag="VFH60_04990"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HET6313170.1"
                     /translation="HGTPGKDMINPTNDLPVIENNGRDVVFMVWGLNRHYLSVFRAYY
                     PGGEEKEFLYGPGENPSNLLTWYRVKSEQVDARRRMQATYTPASGEPVVRDEDGIGTS
                     TPPPDGLTFPATAAWQGSIVAPSMGRSRLAIEAQGAGSLVVNGRTVLTTTAEMPKTET
                     ELLLAKGPHQVTLTGAMRDAQSQVRLLWASVGSELAPVAKQFLWNGPGQGLLGQVSNN
                     QNIDVGAPTTADAGGQPPKVVMSRIDGFLGFRHSPEATIGGPLFATWTGTLNVERPGN
                     YVFDVNSNGDSAVFVDDKLVTLIRASGQPVQSGGQVELASGPHKYELRYTWGSGTGYL
                     EAFWTPPGSERVLIGPDVLRTDAGIIDAATLASEPPPATIDTPGAQPPAPETRQPKAV
                     LGADAGLKEARGLAVDGAGNVYVGDKGNKRVVVLGPDGRVLRMWGGPVPADYKAEQGE
                     APEGTFGDINDIAVAEGADGTTYVYVLDSLQRVQVFTPDGEQVGTYPAAQLTLYGPNG
                     AATGPSGEGGGHRLYISSTGQNRIAVVPSLQTLEAAPRDTTLAKLVESIDGSDADKLD
                     QPVDSVADPANPGILYSIDLKDRIVQLQLQPTPAGTPAAAPRVWRISKQWRVPVGRAE
                     GGSRLAISPDGKHVYMSDPDKHRVAVLDTETNLVSHFGANGRGDGQFAGPSGIAAAPD
                     GTLYVLDRGNNRVQVFEMSDTPK"
BASE COUNT          495 a         1062 c         1034 g          569 t
ORIGIN      
        1 cgctccaacg cgcgtccagg ccgggaacgc cccgcccaaa gaactcttcg cgcggccccg
       61 gcagtaagct cttgcccttc tgccagcccg gcaccccgag gagggccacc cacaaggcca
      121 ccaacccacc ggcccacaac cagcccccca caggctcgac aatattggcc cgcaaggcga
      181 agaacgacca gaccagcagc cccactgcca cgagcaacgc gggcaccgtc acgcgccaac
      241 cctcgcgtgc gcgccaggcg gcgagccgcg ccaccgcctt cgggatgcgt tgccatgcgg
      301 tccttgctac agtggattct gcggttacca gggtaacagt ttgcggggtt gagccgttgt
      361 tgggttgtgc cttgcgtgtc cccaccgggt cggtagacgc gcctggcacc tccggttccg
      421 agcgggctgt cgcgagaggg gtgccattcc ccggggcctc aactgatgag tcctgagtag
      481 cggcaacgga ggcggacccc gaacctgcct tgcgcatggg taacgcggcg ggggtggcgt
      541 tgcttacacg ggcttcgggc ggcgctgcgg gtgaagttgc accaggcgta tgcccgttgc
      601 ccgcagccag gtccaccgca gcagccgctt cgggcgtgtc cacgacggta gcttgcgcgg
      661 tgggggcggc agcgcgtcta cgtggcaggt tggggtgcag cagcgagagg atgaggacaa
      721 ggatcgccag gccgtagcaa aacgttgaga aggtgtaatc gtcccggtcc atgagggcgc
      781 gttggccgta gtaagctatc tgaatagcca gtatgccccc aaagaagccc ctggcctgcc
      841 aaacggactt cagcaacccg tgccgaaatg gagactcctc gtccgggggc ggggagtcca
      901 tgacaggggc ctcaggaggg gccgggagcg cggttcgttt atcggccgga tccgaggttg
      961 gcgtggcaac atcaactggt gcctccgcct gtgctgccga gcgtgcgatg ctattcttgc
     1021 cggacacctg cgcttgttcc attacttcgg cgtgtcgctc atctcgaaca cctgcacccg
     1081 gttgttgccg cgatccagca cgtacagcgt accgtcaggg gctgccgcga taccgcttgg
     1141 cccggcaaac tggccatctc ccctgccgtt ggccccaaag tggctcacca ggttggtctc
     1201 ggtgtcgagc actgctaccc ggtgcttgtc cgggtcgctc atatagacgt gcttgccatc
     1261 ggggctgatg gccagccgcg acccgccctc cgcacgcccc acgggcaccc gccactgctt
     1321 gctgatacgc cacactcgcg gtgcagcagc aggagtaccg gcgggcgtcg gttgcagttg
     1381 aagctgaacg atacggtctt tcaggtctat gctatacagg atgccggggt tggccgggtc
     1441 tgccacgcta tccacaggct ggtccagctt gtcagcatcg ctgccgtcga tgctctcgac
     1501 cagcttcgcg agcgtggtgt ctcgcggcgc ggcctccaac gtctggagcg aagggacgac
     1561 ggcgatccgg ttctggcccg tagacgaaat atacagcctg tgcccgccac cttctcccga
     1621 cgggccggtt gccgcgccgt tggggccgta cagggtaagc tgggcggcag ggtaagtgcc
     1681 cacctgctcg ccgtcggggg tgaagacctg cacccgctgt agcgagtcga gcacgtacac
     1741 gtaagtggtg ccgtccgcgc cctccgccac cgcgatgtcg ttgatgtcgc caaaggtgcc
     1801 ttcgggcgct tccccctgct ccgccttgta gtcggcgggc accggccctc cccacatcct
     1861 gagcacccgc ccgtcggggc cgagcaccac gacgcgcttg ttgcccttgt cgcccacgta
     1921 cacgttaccg gccccgtcca ccgccagccc gcgcgcctcc ttcagcccgg catcggcgcc
     1981 cagcacggcc tttggctgtc gcgtctcagg cgcgggaggc tgtgcgccgg gcgtatcgat
     2041 ggtggccggg ggcggctcgc tggcgagagt ggcggcgtcg atgataccgg cgtcggtacg
     2101 cagcacatcc ggcccgataa gcacgcgctc gctgccgggc ggcgtccaga acgcctcaag
     2161 gtaccccgtg ccggaccccc acgtgtagcg cagctcgtac ttgtgcgggc cgctcgcaag
     2221 ctcgacttgc cctcccgatt gcacgggctg gccgctggcg cggattaggg tgaccagttt
     2281 gtcgtccaca aacaccgcgc tgtcgccgtt ggaatttacg tcgaatacgt aattgcccgg
     2341 ccgctcgacg ttcagggtgc ccgtccaggt ggcaaacagt ggcccgccga tggtcgcttc
     2401 ggggctgtgc cggaacccga gaaaaccgtc tatccggctc attacgactt tgggcggttg
     2461 cccacccgca tcggcggtag tgggagcgcc gacatctata ttctggttgt tgctcacctg
     2521 ccccaacagg ccctgccccg gcccgttcca caggaattgc ttagccacgg gggcaagctc
     2581 agagccgacc gaagcccaca gaagcctcac ctgggactga gcgtctcgca tggccccggt
     2641 tagcgtcacc tggtgcggtc ccttagccag cagcaactcc gtctccgttt tcggcatctc
     2701 agcggtggtg gtaagcactg tgcggccgtt gacgaccagg ctgcccgccc cctgcgcctc
     2761 tattgcgagg cgggagcggc ccatgctggg ggccactatc gagccttgcc aggcggcggt
     2821 agcggggaag gtcaggccgt ccggtggcgg ggtggaggtg ccgatgccgt cttcatcgcg
     2881 cactacaggc tcgcccgatg cgggtgtgta ggtcgcctgc atgcggcggc gggcgtccac
     2941 ctgttcgctc ttgacgcggt accaggtgag cagattactt gggttctcgc cggggccgta
     3001 aaggaactcc ttctcctcgc cgccggggta ataggcgcgg aacaccgata gatagtgcct
     3061 gttcaagccc cacaccatga agaccacgtc tcgcccgttg ttctcaatta cgggcaggtc
     3121 gttcgtgggg ttgatcatgt ccttaccggg ggtgccatga
//
LOCUS       DASRWZ010000322         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1055050, whole genome shotgun sequence.
ACCESSION   DASRWZ010000322 DASRWZ010000000
VERSION     DASRWZ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055050"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFH60_04995"
     CDS             complement(<1..307)
                     /locus_tag="VFH60_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018756534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET6313171.1"
                     /translation="MTTPRLQIGIIPSILSADFARLGEQVRDAEAAGAARIQVDAMDG
                     HFVPNITVGPLVIKGIRPHTTLPIEAHLMIEHAELYIPDFVKAGADYIIVHVEAVTHL
                     "
     gene            complement(991..1749)
                     /gene="pgl"
                     /locus_tag="VFH60_05000"
     CDS             complement(991..1749)
                     /gene="pgl"
                     /locus_tag="VFH60_05000"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET6313172.1"
                     /translation="MSHPHVTVSIEDDADAIAARSAEIFAQLVEAQVADQPFRVALSG
                     GSTPKLLYKLLASEEYRDRVAWDRVQFFFGDERWVPHTDKDSNYKLANDYLFQPLGIE
                     PSRVFPMPTEDVRPPQAAERYEATIRREFALASREMPRFDLVFLGMGDDGHTASLFPH
                     TAALREYDKLVAANYVEKLNTWRITLTAPVLNAAGHVLFMVAGESKAAALKQVLEGDY
                     DPEEYPSQLLREASGHVTWLVDRAAAAQLESGAD"
BASE COUNT          345 a          619 c          587 g          373 t
ORIGIN      
        1 gcaggtgggt caccgcctcg acgtgcacga tgatgtaatc agccccggcc ttcacgaaat
       61 cggggatgta caattcggcg tgctcgatca tcaggtgggc ctcaatcggc agcgtcgtgt
      121 gcggcctgat gccctttatc accagcggcc caaccgtgat attgggcaca aaatggccgt
      181 ccatcgcatc cacctggatg cgggccgcgc ccgccgcctc cgcgtcgcgc acctgctcgc
      241 ccagccgggc gaagtcggcg gacaggatgg aggggatgat gccgatttgt agtcgtggcg
      301 tagtcatgtg gagtattccg tcgtgcccgt acaggtactt gttgaaagac cgaacttgcg
      361 tctcatccgc ttgtctttga tgccgttgca ccggcccaat tggtgactcg gcggcacgcc
      421 caccatgtcg caatagtagc tattagcgcc gggaccaccg cccatacgag ccagccccat
      481 tctccgcccc agcccctacc aacgtattgc ggtaagccac tgaccaccgc aagtagtaaa
      541 gctgccgttc cgccgacaag tatgctcgta atctctgtag cccgccggta gcggcggaaa
      601 tccgggattg gagtaaaagc tcggcacgtc atgattgcca acactgcgcc cgtgacgagg
      661 ccgaggacta tgcctgcgat accccctatc ccaaatccga gtagaaaggc cagtgcgatc
      721 cacatttcca cgccgaccca aatgccgagc ataggttcat ctggagtgtc gccgtcacca
      781 ggtagtaggg cgagcaactg tataaatact gcggccacta gaggaaagat agcacccaac
      841 aaagcgccca gacccgcacc ccagccaacc atgcgccaga tgatgcccag gcacacttgc
      901 ttggcctcca tctccctggt ctaacctcct gtgagtccgt ttgtggcgct cttgttaccc
      961 ggctacttta tgccggtgca agagttgcgc ttagtccgcc ccgctctcca attgcgccgc
     1021 cgccgcacgg tccaccagcc aggtgacgtg gccgctcgcc tcgcgcagta gctggcttgg
     1081 gtactcttcg gggtcgtaat cgccctccag cacctgcttg agcgcggcgg ccttgctctc
     1141 tccggccacc atgaacagca cgtgcccggc ggcgttcagc acgggggcgg tcagcgtgat
     1201 gcgccaggtg ttgagctttt cgacgtagtt agcggcgacg agcttgtcgt actcgcgcaa
     1261 cgcagccgta tgggggaaga gcgaggctgt atgcccgtcg tcccccatgc cgaggaacac
     1321 caggtcgaag cggggcatct cgcggctcgc cagggcgaac tcgcgccgta tcgtggcctc
     1381 gtaccgttcg gcggcctgcg gtggccgcac gtcctcggta ggcatgggga agacgcggct
     1441 cggctcgatg cccagcggtt ggaacaggta gtcgttcgcc agcttgtaat tgctgtcctt
     1501 atcagtatgg ggcacccagc gctcgtcacc gaagaaaaac tgcacccggt cccacgctac
     1561 ccggtcgcgg tactcttccg aggcgagcaa cttgtacagc agcttgggcg tggagccgcc
     1621 ggacagggcc acgcgaaagg gttggtccgc tacctgtgcc tctacgagtt gggcgaatat
     1681 ctcagccgaa cgcgcggcta tcgcatccgc atcatcctct atgctgaccg tgacgtgtgg
     1741 gtggctcaag ggatgttacc tcgtggggtg ttaccgggcg agccggggga ccccggcggg
     1801 cggcgttgcg ggttctgggg ctgcgcggag ctgatcgcct cgccgctgat gagcctgcgg
     1861 gggccgtccg tgtcgcgccc cgacgagact cccttgatga acatagcgac cctgttcatc
     1921 gtct
//
LOCUS       DASRWZ010000323         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_986576, whole genome shotgun sequence.
ACCESSION   DASRWZ010000323 DASRWZ010000000
VERSION     DASRWZ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986576"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..2146)
                     /locus_tag="VFH60_05005"
     CDS             complement(134..2146)
                     /locus_tag="VFH60_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313173.1"
                     /translation="MALCLLGGGEGADVGREAGRGKVAPALMAVRQPRTPATQAVHLP
                     DADANPRKSRAIWTHLLVLGGFVALTIALTWPVASRLFTEVPAGGDAWQHIWNLWWVK
                     QALLVEHTNPYHTGLLYYPDGVSLYFHTLVLTAGLVGIPLQLLGFNLIATYNLILLST
                     FVLAGYGMYLLCMYLTRHHWASFVGGVVFAFSPYHFAHLFGHMNLASLQWLPFYVLAL
                     LKAFEPGPIWNWRQPPALAGDGLTPATSAPQARHVRTRLLWAVAAGALLALNAYTDWL
                     YLIFLVLFTGLFVAWNLLVPSERRGLGLGVRPWVEAGTRLAIVGVVFLVLSAPIFFPT
                     LAEASRGYAQQPPFEVLVYSADLTSAVTPSELHPMWGAAIKEQVNNTGPFLPIKNPSE
                     RVLFLGFSVLALALLGLWRGWRSMQVRFWAFTALATWVLSLGPMLQYLGKTKFTLFEV
                     TVPLPYLLLYKLPLLSIMRTPARLTVLTMLALGVLVAFALAALLGRRYLERGRIFTLR
                     NVVWGLLLPAIIVFEFLATFPTVPPGWNVPIYRQIASEPGRFALLELPIRPFGDYMAY
                     QTIHGKPIVGGYLSRQPPYPLLAETPALHYLEDPVAPDDPVSAQVSGGRGAAALKALG
                     IKYVIVRWWAFTPEQKEVMQAKLNNLLARPPDYSYPSDTVDVWELK"
     gene            complement(2065..>3105)
                     /locus_tag="VFH60_05010"
     CDS             complement(2065..>3105)
                     /locus_tag="VFH60_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6313174.1"
                     /translation="ILVLLLSFYFMKDGDKLFGGIMQMLPPTWQDEVRLAGLSIEKSF
                     GGFIRGQLVFALVYAIFTAIIMLMPPFQLDFVIIASIVAGLCMIIPLIGNFLAFLPAI
                     LVALVTGRTDIWLWLLLVEFIMQSFMMNVLGPRIMSSAIGIHPLYVVAAMLVGGQVAG
                     LWGALFGIPIAGAINLVGRPVMRRIRYQTNLYKDPELPTLPTSAFLTGPLAMSMVQMQ
                     TNPTVRPPGAPAEQGTEEVLMGASQALPARPTGPPATGSTGAPAGQGPQTGSPATMGS
                     ATQRTQSGPLDTGGLGRTNMLNPPAPDVEELYMVPRTPTFSMRVWRFVFSAAGRARTW
                     AGKRAEARLHRR"
BASE COUNT          595 a          942 c         1057 g          511 t
ORIGIN      
        1 gaacctttat tatccctgcg tgccctctgc gtcctctgcg tctctgcggt gaaccgtact
       61 tatttcttct tggcgctctc ttggcgtcct tggcgtcttg gcggttccgt ctataacggg
      121 atcgcttgtg tttctacttc agttcccaca cgtcaaccgt gtccgagggg taggagtagt
      181 cggggggccg ggccaggagg ttgtttagct tcgcctgcat gacctctttc tgctcgggcg
      241 tgaaggccca ccagcggacg attacgtact tgatgcccag ggccttgagc gctgccgctc
      301 ccctcccgcc ggagacctgg gcggacacgg ggtcgtcggg ggccaccggg tcttccaggt
      361 aatgcagcgc gggggtctct gcgaggaggg ggtaaggggg ctgcctggac aggtagccgc
      421 ccactatggg tttgccgtgt atcgtctggt aggccatgta gtccccgaac ggcctgatgg
      481 gaagctccag cagggcgaag cggcccggct cggaggcaat ctggcggtag atgggcacgt
      541 tccagccggg tgggaccgtc gggaacgttg ccaggaactc gaaaacgatt atcgcgggca
      601 gcaacaggcc ccacaccaca ttacgcaatg tgaaaatgcg cccgcgttcc aggtagcgcc
      661 gccccagtag agccgcaagc gcgaacgcca caagcacgcc cagcgcgagc atggtaagca
      721 cggtcaggcg ggcgggcgtg cgcatgatgc tgagcagggg cagcttgtag aggagcaggt
      781 aaggtagcgg caccgtgact tcaaacaggg tgaatttggt cttgcccagg tactgcaaca
      841 tcggccccag gctcagcacc cacgttgcga gtgcggtgaa cgcccagaag cgcacctgca
      901 tcgaccgcca cccacgccac aggcccagca gcgccagcgc cagcaccgag aagccgagga
      961 acagcactct ctcgctgggg ttcttgatag gcaggaatgg ccccgtgttg ttcacctgct
     1021 ctttgatagc cgcgccccac atcgggtgta gctcggaggg ggtgaccgcg gaggtaaggt
     1081 ccgccgaata gaccagcacc tcgaagggcg gctgttgtgc gtagcctctc gacgcttcgg
     1141 ccagagtggg gaagaagatg ggcgcgctca acaccaggaa caccacgccc actatcgcca
     1201 gccgggtgcc cgcttccacc cacgggcgca ctcccaaacc caggccgcgc cgctccgagg
     1261 gcaccagcag attccaggcc acgaacagcc ccgtgaaaag caccaggaag atcaggtaca
     1321 gccagtctgt gtaggcgttc agcgcgagca acgcacccgc cgctactgcc cacagaagtc
     1381 gcgtgcgaac gtggcgggcc tgaggagcgg aagtagccgg agttaagccg tcgccggcca
     1441 atgccggagg ttgtcgccag ttccatattg gcccaggctc gaacgctttc aagagggcca
     1501 gcacgtagaa cgggagccac tgcaaggagg ccaggttcat gtgcccgaac aggtgggcga
     1561 agtggtaggg ggagaaggcg aacacgacac cgcccacgaa ggacgcccaa tggtgccgcg
     1621 taaggtacat gcagagcagg tacatgccat accccgccag cacgaacgtg ctcagcaaga
     1681 taaggttgta cgtggcaatc aggttgaagc ccagcagttg cagcgggata cccacgaggc
     1741 ccgccgtgag cacgagcgtg tgaaagtaga ggctcacccc gtcgggatag tagagcaggc
     1801 cggtatggta ggggttggtg tgctcgacca gcagcgcctg cttgacccac cagaggttcc
     1861 agatgtgctg ccaggcgtcg ccgcccgcgg gcacctcggt gaacaggcgg gaggctaccg
     1921 gccaggtgag ggcgattgtc agcgccacga agccgccgag cacgagcagg tgcgtccaaa
     1981 tcgctctgga tttcctgggg tttgcgtctg cgtcgggaag gtgtacggcc tgcgtggccg
     2041 gcgtgcgggg ctgtcgaacg gccattagcg ccggtgcaac cttgcctcgg cccgcttccc
     2101 ggcccacgtc cgcgccctcc ccgccgccga gaagacaaag cgccacaccc gcatgctgaa
     2161 ggtcggggtg cgcggcacca tgtaaagctc ctcgacgtcc ggggcgggcg ggttgagcat
     2221 gttagtcctg ccgaggccgc cggtatctag tggccccgac tgcgtgcgct gtgtggcgct
     2281 gcccatagtc gcggggctgc cggtctgcgg gccctggccg gcgggagcgc cggtggagcc
     2341 tgtagccggg gggccggtgg gacgtgccgg aagggcctgg gatgcgccca tgagcacctc
     2401 ttcagtgcct tgttcggcgg gcgcgccggg gggccgcacg gtcgggttgg tctgcatctg
     2461 gaccatagac atggcgagcg gccccgtcag gaatgcgctg gtggggaggg tgggtagctc
     2521 cgggtctttg tagaggttag tctggtatcg tatgcggcgc atcacagggc ggcccaccag
     2581 gttgatagcc ccggcgatgg gtatgccgaa gagcgcgccc cacagccccg ctacctgccc
     2641 gcccaccagc atcgcggcca ccacgtacag ggggtgtatg cctatcgcgc tagacatgat
     2701 gcgcgggccg agcacgttca tcatgaacga ctgcatgatg aactccacaa gcagcagcca
     2761 gagccagatg tcggtccgcc ctgtgacgag cgccaccagg atagcgggga gaaacgccag
     2821 gaagttgcct atcaagggga tgatcatgca caacccggcg actatggagg cgattatcac
     2881 gaaatcgagc tggaagggcg gcatcagcat gatgatagcg gtgaagatgg cgtacacgag
     2941 cgcgaacacc agttggcccc ggatgaagcc gccgaagctc ttctcgatgc tcaggcccgc
     3001 aagtcgcacc tcgtcctgcc aggtaggcgg gagcatctgc atgatgccgc cgaagagctt
     3061 gtcgccgtcc ttcatgaagt agaaagatag gaggagcacc aggat
//
LOCUS       DASRWZ010000324         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_370505, whole genome shotgun sequence.
ACCESSION   DASRWZ010000324 DASRWZ010000000
VERSION     DASRWZ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370505"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFH60_05015"
     CDS             complement(<1..442)
                     /locus_tag="VFH60_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6313175.1"
                     /translation="MKALRIHKPKDVRVDNVDDPTIEDPRDAIIRVTSTAICGSDLHI
                     YNGLVPQPRPMSLGHEFMGIVEDIGAEVTNLKKGDRVVVPFPISCGRCYFCTRHLPTA
                     CENSNPNYGARSGLLKEKGGALFGYSDLYGGYEGGQAEAVRVPYA"
     gene            complement(592..1506)
                     /locus_tag="VFH60_05020"
     CDS             complement(592..1506)
                     /locus_tag="VFH60_05020"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HET6313176.1"
                     /translation="MAKNNGRSNGRSNGRRDEVKGSLIIIGGREQKEGDTVILREVAR
                     RAKEGKGLVLVTVATNEPEELAEDYRKAFAELGLKSIDVLDIRTREEAHDPENIKKVI
                     KCSVVFFTGGDQLRITSQIGDSPIFQCLHDIFLNGGTIVGTSAGAAAMSGTMLISGRG
                     DETVEISAIGMAPGLRLLPDVVIDTHFAERGRIGRLVGAVAQNPANLGIGIDEDTAII
                     VNGEEDFLVIGSGGVYVVDGTEISYSSLSEAKPVGSPSIFGVKLHILGEGDIYDLVNR
                     EPVFSGEELEAEVPAEPERELVGANDEK"
     gene            complement(1556..2677)
                     /gene="iadA"
                     /locus_tag="VFH60_05025"
     CDS             complement(1556..2677)
                     /gene="iadA"
                     /locus_tag="VFH60_05025"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019378764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HET6313177.1"
                     /translation="MFILVENGDVYAPESLGKQTILVVGGKIARIGEVDALAVSATGL
                     ECTAVDATGCLVVPGFIDPHQHISGAGGEEGFSSRTPEVQISEIAGAGITTVVGLLGT
                     DATTRNLQSLLAKARQLEQEGVTTYVYTGSFQVPVRTLTGSVTDDLVLIDKVIGVGEL
                     ALSDFRSPEPSLHELARVVSEAITGGLVGGKAGVTHFHTGPGKGRLGLLHRLLDEYEV
                     PPRYLYPTHIERSEGLMDDAIALAKRGAFVDIDVVEPGLGKWLQYYRDNGGPLSQLTV
                     SSDAHASTSANRLHDDFASCVQAHKLPIEEVLPHFTANTATALALHNKGRIREGMDAD
                     ILVLKKRSLEVVHLIANGKQLVKNGKVLARSEYEGLAQG"
BASE COUNT          474 a          854 c          904 g          582 t
ORIGIN      
        1 ctgcgtaggg cacgcgcacg gcttcagcct ggccgccctc gtagccgccg tagaggtcgg
       61 agtagccgaa gagcgcgccg cccttctcct ttagcagccc ggagcgcgcc ccatagttgg
      121 ggttggagtt ctcgcaggct gtgggcaggt ggcgggtgca gaagtagcac cgcccgcagg
      181 agatggggaa gggcaccacc acgcggtcgc ccttcttgag attggtcacc tccgcgccga
      241 tatcctccac gatacccatg aactcgtggc ccagcgacat ggggcgaggc tgcggcacga
      301 ggccgttgta gatgtggagg tcggagccgc agatggctgt ggaggtaacg cggatgatag
      361 cgtcgcgagg gtcctcgatg gtggggtcgt ccacgttatc gacacgtacg tctttgggct
      421 tgtgtatcct gagtgctttc atgacgtagc ctcctccttg aaatgggtat gatgcaggcc
      481 gggagcaccg cctgatactc atcaccttaa gggtccagga taaggttaca agggagcggg
      541 ggcacgctga atcatgccct tacattcttc ctaagagtca agcctgggcg cttacttctc
      601 gtcgtttgcg cccacaagct cacgctcagg ctctgccggt acctctgcct ccagttcctc
      661 ccccgaaaat accggctcgc ggttcacaag gtcatatatg tcgccctcac ccaggatgtg
      721 cagtttcacg ccgaagatgg aggggctgcc taccggcttg gcttcggaga ggctggagta
      781 gctgatctcg gtgccatcca ccacgtagac gccgccggag cctatcacca ggaagtcctc
      841 ctcgccgttg acgatgatgg cggtatcctc gtcgatgccg ataccgaggt tggcggggtt
      901 ctgggccacc gcgcccacca ggcggcctat gcggccacgc tccgcgaagt gcgtatcgat
      961 taccacgtcg ggcaggagcc tcaagccggg ggccatgccg atagcggaga tctccactgt
     1021 ttcatcgccg cggcccgata tgagcatggt gccgctcatc gcggcggccc cggctgaggt
     1081 gcccacgatg gtgccaccgt tcaggaagat gtcatgcagg cactggaata tgggtgagtc
     1141 gcctatctgg ctggttatgc gtaactggtc gccgccggtg aagaagacga ccgagcactt
     1201 tatgaccttc tttatgtttt cagggtcgtg ggcctcttcg cgagtgcgta tgtccagcac
     1261 gtcgatgctc ttcaagccaa gctccgcgaa cgccttcctg tagtcctccg cgagttcctc
     1321 cggctcgttg gttgcgacgg tcacgaggac cagcccctta ccctctttgg cccggcgcgc
     1381 cacctcccgc aggatgaccg tgtctccctc tttctgttcg cgcccgccga tgatgattag
     1441 cgagcctttc acctcatcac gcctgccgtt acttctgccg ttacttctgc cgttgttctt
     1501 agccacagat tcctcctgct taccccccgt agatgtaaaa ggcgaatgac ccggttcacc
     1561 cctgggccag gccctcgtat tcggaccttg ccaggacttt gccgttcttc accagttgct
     1621 tgccgttggc tatgagatgc actacttcca gcgaccgctt cttcagcacc aggatatcgg
     1681 cgtccatgcc ttcgcgtatg cggcctttgt tgtgcagggc cagggcggtg gccgtgttgg
     1741 cggtgaaatg tggaagtacc tcctctatgg gcaatttgtg tgcctgtacg caggaggcga
     1801 agtcgtcgtg cagcctgttg gcggaggtgc tggcatgggc gtcggaggag acggtaagct
     1861 gagagagggg accgccgttg tcgcggtaat attgcagcca cttgccaagc cccggctcga
     1921 ccacgtcgat gtccacgaac gcgccccttt tggcgagggc tatagcgtcg tccatcagcc
     1981 cttcgctgcg ctcgatgtgg gtgggataga ggtagcgggg tgggacctcg tactcatcca
     2041 gcaggcggtg gaggaggccc aggcggcctt tgcccggccc cgtgtggaag tgggtcacgc
     2101 ccgccttacc gcccaccagg ccgccggtaa tcgcctccga gacgacccgc gcaagctcgt
     2161 gcagggacgg ttcgggcgag cggaagtcgg agagggccag ctcgcccacg cctatcacct
     2221 tgtcgatgag caccaggtcg tcggtgacgg agccggtgag ggtacgcacc ggcacctgga
     2281 agctgccggt gtagacgtag gtggtgacgc cctcttgctc tagctggcgg gccttggcga
     2341 gcagggattg caggttgcgg gtggtggcgt cggtgccgag gaggccgacc accgtggtga
     2401 tgcccgcgcc cgcgatttcg ctgatttgca cttcgggggt gcggctggag aagccttcct
     2461 cgccgcccgc gccgctgatg tgctggtggg ggtcgatgaa gccgggcacg accaggcagc
     2521 cggtagcgtc cacggcggta cattccagcc ccgtggccga aaccgccagg gcatcgacct
     2581 caccgatacg cgcgatcttg ccgcccacca caagtatggt ctgcttgccg aggctttcgg
     2641 gggcgtacac gtcgccgttt tcaaccagga tgaacacatg atgctccatg cgaaaggtcg
     2701 cacgaggcta gggggcaagt gtcatgccag acgagtaatg agtgatgagg actgaggact
     2761 gagaggctcc gatccaaatc attacctgcg tggcgcgctc ttagtgtgca actt
//
LOCUS       DASRWZ010000325         5023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_95980,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000325 DASRWZ010000000
VERSION     DASRWZ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95980"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..3072
                     /locus_tag="VFH60_05030"
     CDS             322..3072
                     /locus_tag="VFH60_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6313178.1"
                     /translation="MFRLPGLVSIVSLVLLALFAVGSGVPAAAVGASFSAEPLVRGAD
                     YRLGGGAYKSGYSINSTDKSAQPANGQGLGSDLTLPAGQHRETFTSGVVEAPLDFTDV
                     APHWWADVPEQTYVLVEVRTSRDAQTWADWEPADLEDIIMPMDAVTQTYASTISVPQA
                     ERTHRFVQSRVTLSTNYLNRTPVFHELTYTFIDAGVTANPPKPQGATVQSLAADVPKP
                     RVVPRQDWGAPNADSSPRWKPKFRRVTHIVIHHTATPNNDTDWAARVRAIWYYHANTR
                     GWGDIGYNYLVDPNGVIYEGRFGGDDVEGGHTYPFNTGSMGIGMIGNFMTVAPSASAQ
                     SALIDLISWKATQRGIDPHGSGPLTGYTNCGGVMTYTRPNIAGHRDYKGNTCGRDFNS
                     STCPGDKLWDMLPQIRDSIVAEQPPLRATFTQHDTPGNLTPGSNINVKLTVRNSGSLT
                     WPAQGQGAVSVGYRWLGADGKPLKGAPEARTPLTRNVPFADTLVITAKLSVPPLNGKY
                     ALIWDMYQDGVGWFSDQGTSAPLRVDVVLGRSPGDKLAPTSSVLPIPIYSNNPEFLVR
                     WAGQDEPNGSGLASYDIQQRIAPNGTWTDWQSATAQTQAVFEGQDGYTYEFRSRARDA
                     AGNVEAWPDKAQAYTTVDMRPPPLIIDSPQHGAHVQPGPLQVVGKTEPGAFVAVNDTR
                     AQEANGVFTATVQAEGRDFIIHVTASDAAGNVSRQEVVVQAASRFSDVPLTDPASEAV
                     EALSDRGIITGYSDGSFKPDAEVTRAQFAKFMAVVMRWALIKPPEPRFSDVPADSALF
                     SYVETATARGSMRGYTDGTFKPNEGVLRAEAVQNWVRAAGWKPDYEGSGIFLDVPAGY
                     PGAIYIETAFAHGIIAPDDEGNFRPTDTITRAELSQMVYEYLKVTKQTEVEYPSVEGG
                     PE"
     gene            3594..>5023
                     /locus_tag="VFH60_05035"
     CDS             3594..>5023
                     /locus_tag="VFH60_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313179.1"
                     /translation="MYRKLLAAAFTVIFALIASAWILKTTAYGATYSPAESNAAQNNK
                     PAPTAAGSQPDAPSAMGSQSEPSVAYNPTATEYLVVWEDNRGTGANGAPNVYGQRVDA
                     DGTMLGTNFVIAEAAGAQEKPKVAFSTDATGYLVVWKDSRADSTGDIYGQLVNSDGTL
                     FEVNFPISTVPGSGEAFPDVAYSASSNEYLVVWSISVSNSNRDVKGLRLNTEGTPQVG
                     VINIMQTSSVEFATSVSYNASTTQFLVVAESRTSFAFGTNNVHGQLVGSNGALSGQTF
                     PIAANPDVLESDPDVAYNSTLHDWLVVWRDTREVTGDVYGRRIGSNGSLLGSDFAIAA
                     ANSTQERPSVAYSNAAQVYMVTWHDARVQYADIYAQIVHNNGVLSGANFIVSNAAGMQ
                     AGPDIVDSGGPTFFFAVWTDSRDNDSDVFAQRISNNGQMLDANFPVAPYPYEPNTPTP
                     TRTSTASPTAPPTATSTPCTIQYSDVP"
BASE COUNT         1028 a         1655 c         1452 g          888 t
ORIGIN      
        1 agggcagact ttgtaatgtg tagccctcga tttcaatcga cgggcgtcgg ctttgtgtgc
       61 tgccgcgcca tttgcttgct tacccggtgg cctttgcatg ctgttgtatt tcgatgccag
      121 gcctcatctc gcaaggatgc aagccctcgc ggctaacgca ccccctcgtt gacggcaccc
      181 tgtttccaca atatactcgg ctttggtccc ttcttcctgc accagagcgg aggcttcttc
      241 atgcgcttac agcgcacccg tcgcaaacac aacccaaagc gctcgtaccc actgtggcaa
      301 ccgcaaccct cacggcaccc cttgttcagg cttcccgggc tggtctccat cgtctccctg
      361 gttctgctcg ccctgttcgc cgtaggttcc ggcgtgcccg ctgccgccgt gggagcttcg
      421 ttttccgctg agcctcttgt gcggggggcc gattacaggc tgggaggagg cgcgtacaag
      481 tcggggtaca gcatcaacag cacggacaag agtgctcagc ccgcgaacgg gcaagggttg
      541 gggagcgacc tgacgttacc ggcgggccag catagagaga ccttcacctc cggcgtggtg
      601 gaggccccgc tcgacttcac cgatgtcgcg ccccactggt gggccgacgt gccggagcag
      661 acttacgtgc tggtggaggt gcgcaccagc cgggacgcgc agacctgggc cgactgggag
      721 cccgccgacc tggaagacat catcatgccg atggacgcgg tgacgcagac ctacgcctcc
      781 accatctcgg tgccccaggc ggaacgcacc caccgcttcg tccagagccg cgtcaccctg
      841 tccaccaact acctcaatcg cacccccgtg ttccacgagc tgacctatac cttcatcgac
      901 gcgggcgtga ccgcaaaccc cccgaagccg cagggagcca ccgtgcagag cctggcggcc
      961 gatgtgccca agcccagggt agtgccgcgc caggactggg gcgcacccaa cgccgatagc
     1021 agcccccgct ggaagcccaa gttccgccgc gtgacccaca tcgtcatcca ccataccgcc
     1081 acccccaaca acgacactga ctgggccgcc cgcgtgcgcg ccatctggta ctaccatgcc
     1141 aacacgcgcg gttggggcga catcggctac aactacctgg ttgaccccaa cggcgtcatt
     1201 tacgaggggc gcttcggcgg cgacgacgtg gagggcgggc acacttaccc cttcaacacg
     1261 ggcagcatgg gcatcggcat gataggcaac ttcatgacag tggctcccag cgcctccgcc
     1321 cagtccgcgc tgattgacct gatttcgtgg aaggctacac agcgcggcat agacccccac
     1381 ggttccggcc cccttacagg ctataccaac tgcggcggcg tgatgaccta cacccgcccc
     1441 aacatcgccg ggcaccgcga ctacaagggc aacacctgtg ggcgcgactt caactcctcc
     1501 acctgtcccg gcgacaagct gtgggacatg ctgccccaga tacgcgactc aattgtggcc
     1561 gagcagcccc cgctcagggc gaccttcacg cagcacgaca cccccggcaa cctcacgccc
     1621 ggctctaaca tcaacgtcaa gctgaccgta cgcaactcag gctcgctcac gtggcccgcg
     1681 caggggcaag gtgccgtgtc ggtaggctac cgctggctgg gggccgatgg gaagcctctg
     1741 aagggcgcgc ccgaagccag gacgccgctc acccgcaacg tacccttcgc cgataccctg
     1801 gttatcaccg ccaagctgag cgtgcccccg ctcaacggca agtacgcgct catctgggat
     1861 atgtaccagg acggggtggg ctggttctcc gaccagggca cctctgctcc cctgcgcgtg
     1921 gacgtggtgc tgggccgcag ccccggtgac aagctcgcgc ccacctcctc cgtgctcccc
     1981 atcccgatct acagcaacaa ccctgagttc ctggtgcgtt gggcggggca ggacgagccg
     2041 aacggctcgg gcctggcctc ctacgacatc caacagcgca tcgcacccaa cggcacctgg
     2101 accgactggc agtccgccac cgcgcagacg caggccgtgt tcgagggcca ggacggctac
     2161 acctacgagt tccgctcgcg ggcacgtgac gcggcgggca acgtcgaggc gtggcccgac
     2221 aaggcgcagg cttacactac ggtggacatg cgcccgccgc ccctcatcat cgactcgccg
     2281 cagcacgggg cgcacgtcca gcctgggccg ctacaggtgg tcggcaagac cgagccgggg
     2341 gcctttgtcg ccgtcaacga tacgcgggcg caggaggcga acggggtctt tacagccacg
     2401 gtgcaggccg agggccgcga cttcatcatc cacgtaaccg cctcagacgc cgccggcaac
     2461 gtgtcgcggc aagaggtggt cgtgcaggcc gcctcccgct tcagcgacgt gcccctcacc
     2521 gacccggcct ccgaggcggt ggaggcgctg agcgataggg gcatcatcac cggctactcg
     2581 gacggctcgt tcaagccgga cgccgaggta acgcgggctc aattcgccaa gttcatggcg
     2641 gtcgtgatga ggtgggcctt gatcaagccc cccgagccac gcttcagcga cgtgcccgcc
     2701 gacagcgccc tcttcagcta cgtggagacc gccaccgcgc gcgggtccat gcggggctac
     2761 acggacggca cgttcaagcc caatgaaggc gtgttgaggg cggaggcggt ccagaactgg
     2821 gtccgcgcgg cgggctggaa gccggactac gaaggttcgg gtatcttcct ggacgtgccc
     2881 gcgggctatc ccggcgctat ctacatcgag accgcattcg cccacggcat catagctccc
     2941 gacgacgaag gcaacttccg ccccacggac accatcaccc gcgccgagct gagccagatg
     3001 gtctacgagt acctcaaggt gaccaagcag acagaggtcg agtacccttc ggtcgaaggc
     3061 ggcccggagt agcgccgggc aagacagctg cattaggccc aatatgcagg ccgcatgtgc
     3121 ggtaccaggc tcccctctgt cattctgagc gtagcgaaga atctaaagaa gttggcacag
     3181 ggagactgtt gaacattaca ccctatagtg ctgacgttcg gtgtaggggc aaaagtagca
     3241 tcctagattc ttcgcggagt ttaccctgag cgcagccgaa ggactcagaa tgacaggggc
     3301 cacccttgtt agcttacttc ctacagtttg caggtagacc ctagtgtttg ctgtgcctga
     3361 ttgccggcca ggttcggcgt tgctcacaca aagagcggca gccaggctgt atatgccgcc
     3421 caggttacgc gaacattatc accccggcct ggattaatta aggtctgctc gggggattta
     3481 attgattcca gtgatgtagt ctcgcttcct gtgcggcaat atgtacatag gcaagaaaag
     3541 ctaatttgcc tgatgcacaa attactagca gaaaaacagc aggagacctc tttatgtatc
     3601 gcaaattact ggcagccgct ttcaccgtga tattcgcact aatagccagt gcgtggatcc
     3661 tcaagactac cgcctatgga gctacatact caccggcaga aagcaacgcg gcacagaaca
     3721 acaaacctgc acctacggca gccggcagcc agcctgatgc accctccgcg atgggaagcc
     3781 agtccgaacc gtcggtagcc tataacccca ccgctactga gtacctggtt gtctgggagg
     3841 ataaccgggg tacgggtgct aacggtgccc ctaacgtgta cgggcagcgg gtggacgccg
     3901 atggcaccat gctcggcaca aatttcgtga tagccgaggc cgctggagca caggagaaac
     3961 cgaaggtagc cttcagcacg gatgctaccg gatacctcgt tgtttggaag gattctcggg
     4021 ccgatagtac cggcgacata tacggccaac tcgtaaactc cgatggaaca ttattcgaag
     4081 tgaactttcc tatctctacg gtaccgggtt cgggggaggc attccctgat gtagcataca
     4141 gcgcctcaag caacgagtac ctggtagtct ggtcgatatc cgtctccaat tccaaccggg
     4201 acgtgaaagg cttacggctc aacaccgagg gcacacccca ggtaggcgtg atcaatataa
     4261 tgcagaccag ctccgtggag ttcgcaactt cggtgtcata caatgcgtcc accacgcagt
     4321 ttttggtcgt tgctgaatcc cggacctcct tcgcctttgg cactaataac gtacacggcc
     4381 aactggtagg cagcaacggc gcactgagcg gacagacttt ccccatagcc gcgaaccccg
     4441 acgtgttgga gtctgacccg gacgtggcat acaactcaac gcttcacgac tggctggtgg
     4501 tgtggcggga cactcgcgaa gtcaccggtg acgtgtacgg ccgccgtata ggttcgaacg
     4561 gttcattact gggatcggat ttcgccattg ccgccgcaaa cagtacccag gagagaccgt
     4621 cagtagccta cagtaacgcg gcccaggtgt acatggtaac ctggcatgac gcccgcgtgc
     4681 agtacgccga catatacgca cagatcgtgc ataacaacgg cgtgcttagc ggtgcaaact
     4741 tcatcgtcag taatgcagct ggtatgcaag ctggaccgga catagtagat agcggtgggc
     4801 caaccttctt cttcgcagtg tggaccgata gccgtgacaa cgacagcgac gttttcgccc
     4861 agcgtatcag caacaacggg caaatgctgg atgccaattt ccccgtagct ccatacccct
     4921 acgaaccgaa tactccaact cccacacgca cctctaccgc cagcccgacg gcccctccaa
     4981 cagccacatc aacaccttgc acgatacagt actccgacgt gcc
//
LOCUS       DASRWZ010000326         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_987120, whole genome shotgun sequence.
ACCESSION   DASRWZ010000326 DASRWZ010000000
VERSION     DASRWZ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987120"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFH60_05040"
     CDS             <1..469
                     /locus_tag="VFH60_05040"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HET6313180.1"
                     /translation="ATCYLPGLRTTDHSALGTRHSALGTNDMEATNAQNRDDRIYTWA
                     AFTLRTGMFASFGAMLVGLVWWLLGGSQGGQEAAQHVLPFDRIVPELLALNPLALINV
                     GVLLLLATPGITLVAQFVAFALARNWIYAGITAFISVAIGFSVALSLGLITLF"
     gene            600..1568
                     /locus_tag="VFH60_05045"
     CDS             600..1568
                     /locus_tag="VFH60_05045"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6313181.1"
                     /translation="MMYQPNSPSPDPQWSPARPPQSEGDDYAPSRPYSPPPTYLPAPV
                     PPPGDMSAARAARTWIRIPVERPLWTGLLLASMIVVFALTTLLSGNLSILPLASEPAD
                     PITIWGAKRNEWVVFDFQWWRLFTVMFLHAGLLHMVTNGYALYIIGNEVEGLFGRARF
                     LAIFFISGLAGSVASVAVSPFNQWGVGASGAIFGLIGALGVYFGLHRTLFGARGNIQF
                     WNIILIVVINLGFGFSGILPIDNSAHLGGLVGGALVGYILCPRYRLGEWEMPNVRGLV
                     DVNKGRLPWITAGVFTLVTVAIFLVLVVLFNQDYIQPDYVMVLVRR"
     gene            1607..>2113
                     /locus_tag="VFH60_05050"
     CDS             1607..>2113
                     /locus_tag="VFH60_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET6313182.1"
                     /translation="MHTQYLWTPWRMAYVSGKDPEETDRAGADEAPTQEAHPVPERDT
                     HEEDPACVFCDRIRLSPDHDRANLILLRAEHNFAILNLYPYNSAHLMLVPYEHTADFA
                     GLHSDTVGEMMALAQRMLAAIKEEFAPEGFNLGLNLGRIAGAGVADHVHMHVVPRWAG
                     DTNFMPIVG"
BASE COUNT          345 a          753 c          612 g          403 t
ORIGIN      
        1 cgcgacatgc tatctacccg gactacggac cactgaccac tcggcactcg gcactcggca
       61 ctcggcactc ggcactaacg atatggaagc aacaaacgca caaaaccgtg acgaccggat
      121 atacacctgg gccgctttca cgctccgcac cggcatgttt gccagctttg gggccatgct
      181 ggtggggctg gtgtggtggc tgctgggcgg gtcgcagggc gggcaggagg cggcgcagca
      241 cgtcctgccc ttcgaccgca tcgtaccgga gctgctggcc ctcaacccgc tcgccctcat
      301 caacgtaggc gtgctgctct tgctggctac gcccggtatc acgctcgtcg cccagtttgt
      361 ggcgttcgcc ctggcgcgta actggatata tgccggtata accgccttca tctcggtagc
      421 catagggttc agcgtggcgc tgtcgcttgg attgataacg ctgttctagg ggcgcgccgg
      481 tgcccgcgtt gaagctgttt gtgcgccaat atcgtatact tgagtgtagc cctcgtatta
      541 ttcacccgca cgcggcccct cctcgaccgc ccttaccggc gctagacctc tttgcctgca
      601 tgatgtacca acccaattcg ccgtcccccg acccccaatg gtcgcccgcc cgcccgccgc
      661 aaagcgaggg tgacgactac gctccgtcgc gcccctactc gccgccgccc acctatctcc
      721 cggccccggt gcctccccca ggcgacatga gcgcggcccg cgccgcccgc acctggatcc
      781 gcatacccgt tgagaggcca ctctggacgg gcctcctgct cgcctcaatg attgtcgtct
      841 tcgccctcac taccctgctc agcggcaacc tctccatact gccactggcc tccgagccgg
      901 cagacccaat taccatctgg ggcgccaaac gcaacgaatg ggtcgtgttt gacttccagt
      961 ggtggcggct cttcacggtc atgttcctgc acgcgggcct gttgcacatg gtcaccaacg
     1021 gctacgcgct ctacatcata ggcaacgagg tggaagggct gtttggccgg gcgcgcttcc
     1081 tggctatctt cttcatatcg ggcctggcgg gcagcgttgc cagcgtggcg gtctccccct
     1141 tcaaccagtg gggcgtgggc gcgtccggcg cgattttcgg cctgataggc gcgcttgggg
     1201 tctatttcgg cctgcaccgc accctgttcg gcgcgagggg caacatccag ttctggaaca
     1261 tcatcctcat agtcgttatc aacctggggt tcggcttctc cggcatcctc cccatcgaca
     1321 actcggctca cctgggcggt cttgtaggcg gggcgctggt aggctacatc ctgtgccccc
     1381 gctacaggct gggtgagtgg gagatgccca acgtgcgcgg cctggtggac gtcaataagg
     1441 ggcggctgcc ctggatcacg gcgggcgtgt tcaccctggt gacggttgcg atcttcctcg
     1501 tgctggtggt gctcttcaac caggactaca tacagcccga ctacgtcatg gtcctcgtca
     1561 gacgctagcg cccggcggcg ggacacccct tcaggagcga catctcgtgc acacgcagta
     1621 cctgtggacc ccctggcgca tggcctacgt ctccggcaaa gaccccgaag agacagaccg
     1681 tgccggggca gatgaggccc ccacccagga ggcacaccct gtgcccgaac gagacacgca
     1741 cgaggaagac cccgcctgcg tgttctgcga ccgcatccgc ctctcccccg accacgaccg
     1801 cgccaacctg atcctgctcc gcgccgagca caacttcgcc atcctcaacc tctaccccta
     1861 caactccgcc cacctcatgc tggtgcccta cgagcacacc gccgatttcg cgggcctgca
     1921 tagcgatacg gtcggtgaga tgatggccct ggctcagcgc atgctagcgg ccatcaagga
     1981 ggagttcgcc cccgaaggct tcaacctcgg cctgaacctg ggccgcatcg cgggcgcggg
     2041 agtggccgac cacgtccaca tgcacgtcgt cccccgctgg gcaggcgaca ccaacttcat
     2101 gcccatagtc ggc
//
LOCUS       DASRWZ010000327         5158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1193115, whole genome shotgun sequence.
ACCESSION   DASRWZ010000327 DASRWZ010000000
VERSION     DASRWZ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193115"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..1085)
                     /gene="trpA"
                     /locus_tag="VFH60_05055"
     CDS             complement(282..1085)
                     /gene="trpA"
                     /locus_tag="VFH60_05055"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET6313183.1"
                     /translation="MGRIANTFRRLKKANEAALVAYVTVGYPSLDLTRRLVPIIGRQG
                     ADLIELGVPFSDPMADGATVQRASHAALEQGVSLSDCLSVAAEARRTNEVPLLFMSYY
                     NPIFKYGIEEFVHDCAAAGVDGLIIPDLPPEEAGELKQACDGAGIDLIFLVAPTSTDE
                     RLRKVAEMASGFVYCVSLTGVTGARSEVGEGVEAMLARVREHTDLPLVVGFGISKPEH
                     VARVSRVADGAVVGSALINVIESHPDEDEVILSVSEYVRDLKESTRRGQ"
     gene            complement(1085..2317)
                     /gene="trpB"
                     /locus_tag="VFH60_05060"
     CDS             complement(1085..2317)
                     /gene="trpB"
                     /locus_tag="VFH60_05060"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET6313184.1"
                     /translation="MPDANGHFGQFGGKYAPEVLMPALDELERAYNLYRSDPDFLTEL
                     RGLEAHYVGRATPLYFAESLTREMGGARIYLKREDLAHTGAHKINNALGQALLARRMG
                     KRRIIAETGAGQHGVATATVCAMLGLECIVYMGEVDIQRQALNVFRMKLLGAEVRPVS
                     SGSRTLKDAINEAVRDWVANVGTTYYLIGSAVGMHPYPTIVRDFQSIIGREARAQSLE
                     QVGRLPDYVVACVGGGSNSIGIFHPFISDQDVRLVGVEAGGEGVGSGKHACTLSAGSV
                     GVLHGSRSYMLQDEHGQVMETHSISAGLDYPGVGPEHSYLRDTGRAQYVGVTDAEALE
                     GFKLLCRTEGIIPALESAHAIYYGANLAASLSPGDMVVINLSGRGDKDMGTVAEALGI
                     TLSQASAPQAPHTGQAKA"
     gene            complement(2370..3065)
                     /locus_tag="VFH60_05065"
     CDS             complement(2370..3065)
                     /locus_tag="VFH60_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET6313185.1"
                     /translation="MRPPARGDRSTAVKICGLMQPQHALVAAEGGADMLGMVFAPSRR
                     QISPDIARSIAAAILVRADRPLLVGVFVNESTARMLEIAEQVGLDLLQLSGDESAEQV
                     GECAAYYPTIKAVRFPTSMPPEQALSDLEPYTRLDLGDRVRLLLDTYSPGIYGGTGET
                     ADWSLAAGIAERLPLILAGGLNPGNVGAAIERVSPWAVDVSSGVETGGVKDRALIREF
                     ISAACHGSVALKG"
     gene            complement(3062..3874)
                     /gene="trpC"
                     /locus_tag="VFH60_05070"
     CDS             complement(3062..3874)
                     /gene="trpC"
                     /locus_tag="VFH60_05070"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET6313186.1"
                     /translation="MSILAEIVANKQEEISFRKLARPLPSILAQLHRATPTRDFREAL
                     SDASKRPPRVIAEIKRRSPSKGALRPDLDPAQVASIYQRNGAAALSVLADSRYFGGSL
                     DDLAVVSMWSSLPVLCKEFIVDAYQVYEARLAGADAVLLLASVLGMEQLRDFREIAES
                     LGMSALVEVHSENELFAALASGAGIVGINNRDLRTFEVSLDTTRRLLPLIPRDVVTVS
                     ESGIRDSSDRETMSALGVDALLIGEGLLTSPDLAAATRRMCGTVATALVAAR"
     gene            complement(4021..5013)
                     /gene="trpD"
                     /locus_tag="VFH60_05075"
                     /pseudo
     CDS             complement(4021..5013)
                     /gene="trpD"
                     /locus_tag="VFH60_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
BASE COUNT          829 a         1746 c         1645 g          938 t
ORIGIN      
        1 agccgtcacc accgcccggc aactaaagga aatgctcgaa aaggcagggg agcgcgtcgt
       61 ctgaagcggg ataaggagac gaaaacgcaa agacgcaaag acgcaaagaa cgcgcaaagt
      121 ttaaggattg tgttctaagt acaaagagga ccagggtacg tcatgttgta tcctggtcct
      181 ctttgtacgc ttaaaccatt cttctctctg cgcgtccttt gcgtctttgc gttttcgtct
      241 ctatattgga actaccgccc aggtgttaca cttctgggtg cttattgccc tctgcgggtg
      301 ctctctttca agtcgcgcac gtactcggac acgctgagga tgacctcgtc ctcgtcgggg
      361 tggctctcta tgacgttgat gagggcgctg cccaccaccg cgccgtccgc cactcgcgac
      421 acccgcgcca cgtgctccgg cttgctgatg ccgaagccca ctaccagcgg caggtcggta
      481 tgctcgcgca cccgcgccaa cattgcctcc acgccctctc ccacctcaga acgcgccccc
      541 gtgacgcccg tgagcgacac gcagtagacg aagccgctcg ccatctcggc cactttgcgc
      601 aggcgctcgt ccgtgctcgt gggcgcgacc aggaatatca ggtcgatacc cgcgccatcg
      661 caggcttgct ttagctcccc ggcttcttcg ggcggcaggt cggggatgat gaggccgtcc
      721 acgcccgcag ccgcgcagtc gtgtacgaac tcctctatgc cgtacttaaa gatggggttg
      781 tagtagctca tgaagagcag cggcacctcg ttggtgcgcc gcgcctcggc cgccaccgag
      841 aggcaatcgc tgaggctgac cccctgttca agcgcggcgt gggaggcgcg ctgcacggta
      901 gccccgtcgg ccatagggtc gctgaagggc acgccaagct cgatcaggtc ggcaccctgc
      961 cggccgatga tgggcaccag gcggcgcgtc aggtcgagcg acgggtagcc cacggttacg
     1021 taggccacca gggcggcttc gttggccttc ttgagacggc gaaatgtgtt ggctatacgt
     1081 cccatcaggc cttcgcctgg ccggtatgag gcgcttgtgg tgctgatgcc tgcgagagcg
     1141 taatgcccaa cgcttcggcc acggtgccca tgtccttgtc gcccctgccg gagaggttga
     1201 tgacgaccat gtcgcccggc gaaagggagg ctgccaggtt ggccccgtag tagatagcgt
     1261 gagccgattc cagggccggt atgataccct cggtacggca gagcagcttg aagccctcca
     1321 aggcctccgc gtcggtgacg cccacgtact gtgcccgccc ggtatcgcgc aggtagctgt
     1381 gctcaggccc cacgccgggg tagtccagcc ctgccgagat gctgtgggtc tccatcacct
     1441 ggccgtgctc gtcctgcaac atgtaggagc gcgagccgtg cagcacgccc accgaaccgg
     1501 cgctaagcgt gcaggcgtgc ttgccgctgc ccacgccctc gccccctgcc tccaccccca
     1561 ccagccgcac atcctggtcg ctgatgaagg ggtggaagat gccgatgctg ttgctgccgc
     1621 cgcccacgca ggccaccacg tagtcgggca accggcccac ctgctcaagt gattgcgccc
     1681 tggcctcgcg cccgatgatg ctctggaagt cccgcactat cgtggggtag gggtgcatgc
     1741 ccaccgccga gccgatgagg taataggtcg tgccgacgtt ggctacccag tcgcgcaccg
     1801 cctcattgat cgcgtccttg agggtgcggc tgccggaaga gactggccgc acctcggccc
     1861 ccagcagctt catgcgaaag acgtttagcg cctgccgctg aatgtccacc tcacccatgt
     1921 agacgatgca ctccaggccg agcatagcgc acaccgtggc ggtggcgacc ccgtgctgcc
     1981 ccgcgcccgt ctccgcgatt atgcggcgct tgcccatacg cctggcgagc agggcctgcc
     2041 ccagggcgtt gttgattttg tgcgctccgg tgtgcgccag gtcctcccgc ttgagataga
     2101 tgcgcgcgcc gcccatctcc cgcgtgaggc tctcggcgaa gtagaggggc gtggccctgc
     2161 ccacgtaatg cgcttcgagg ccccgcagct ctgtgaggaa atcggggtcg ctcctgtaca
     2221 ggttgtaggc gcgctcaagc tcgtccagcg cgggcatgag cacttcgggg gcatacttgc
     2281 cgccaaactg cccgaagtgg ccgtttgcat ccggcatgct atacgtccgg tctgattcta
     2341 tgggcttatc ttgtatgctg accatgatat tatcctttca gggcgacgga cccgtgacac
     2401 gccgccgata tgaactctct tattagcgct ctgtccttca ccccgccggt ctccacgccg
     2461 ctgctcacgt ccacggccca gggggatacc cgctcaatcg ccgctccaac gttgccgggg
     2521 ttcagcccac ccgcgagtat caggggcagt ctctccgcga taccggcggc taaggaccag
     2581 tcggcggtct ccccggtgcc accgtagatg cccggactgt acgtatcgag cagcagccgc
     2641 acgcggtcgc ccaggtcgag ccgggtgtaa ggctccaggt cggatagggc ctgctccggg
     2701 ggcatgctgg tggggaagcg cacggccttg atggtgggat agtaggcggc gcactctccc
     2761 acctgctccg cgctttcatc gccgctcaat tgcagcaggt cgaggcccac ctgttcggca
     2821 atttcaagca tccgcgcggt gctctcgttc acgaacactc ccacaagcag gggcctatcc
     2881 gcgcgcacaa gtatggcagc cgcgatgctc cgggcaatgt ccgggctgat ctgcctgcgg
     2941 ctcggtgcga acaccatgcc cagcatatct gccccgccct cagcggcgac gagggcgtgc
     3001 tggggctgca tgaggccgca aatcttcacg gcggtgctcc ggtcacccct ggcgggcggt
     3061 ctcatcgagc ggccaccagt gccgtcgcga cagtgccgca catccggcgc gtggccgccg
     3121 ccaggtcggg cgaggtcagc aagccctcac cgatgagcag cgcgtccacg ccgagagcag
     3181 acatggtctc gcggtcggag gagtcgcgta tgccgctctc gctgaccgtg actacgtcgc
     3241 gcggtatgag gggtagcagc cgccgggtgg tgtcgaggct cacctcgaag gtgcgcaggt
     3301 cgcggttgtt gatgcccacg atgccggccc ccgatgcgag cgcggcgaat agctcatttt
     3361 cgctgtgcac ctccaccagg gccgacatgc ccaggctctc ggctatctcg cgaaagtcgc
     3421 gcagctgctc catgcccaga acggaggcga gcagcagcac cgcgtcggcc cccgcgaggc
     3481 gcgcctcgta cacctggtag gcgtccacga tgaactcttt gcacagcacc ggcagggagg
     3541 accacatgct gaccaccgcc aagtcgtcca gcgacccgcc aaagtagcgg ctatccgcca
     3601 gcacggagag agcagccgcc ccattgcgct ggtagatgga agccacctgc gcggggtcga
     3661 ggtcggggcg aagcgcgccc ttggaggggc tgcgacgctt gatttcggct atgaccctgg
     3721 gggggcgctt ggaggcgtcg gaaagggcct cacgaaagtc gcgcgtgggg gtcgcccggt
     3781 gcaattgtgc caggatagag ggcagggggc gcgccagctt gcggaaggag atttcttcct
     3841 gtttgttggc gacgatctcg gccaggatgc tcatgcggcc acctccgcgc ctttcttgct
     3901 gtcggcgtgc agcttggcgt tggcggtgag ggccacttcc acttgcaggg ccacgtcggc
     3961 ggtaaggcct tcttcgacct gcggaccgcc ggtgagcacc gggttctggc tgaaccgcgc
     4021 caggcgctcc aacgtctcca tgcccgcgcc gctgtcgata gcggcggcgg ccaagtcaac
     4081 gccgtcgcgc atagagggta ccacgtcggc ggcgaacagg gcggcggccg cgttcatcag
     4141 caccacgtcc cgttgcggcc cgcgctcgcc cgacagcacc gagcggatga tagccgcgtt
     4201 gtcctccgcc gtgccgcccg ctagcgactc gcgtgaggcg cgctccaggc caagctcctc
     4261 cggggtgact tccaggcgct cggtaggctg ccccgcccgc accctgtaaa gcaccgacgg
     4321 ccccgtgatg ctcaactcgt ccatgccgcc gtcgccgtgc accaccaacg cgtgctgcgc
     4381 gccaagcaac tcaagcaccc ccgccatctt gggggcgagc gcgccatcgg ccacgccaag
     4441 cacctgcgag cgggcacctg cggggttggt gagggggccg aggatgttga acacggtgcg
     4501 ggtgccaagc tcgcggcgga ccggggcggc gtgcttcatc gcggggtgga acaggggcgc
     4561 gaacatgaag ccgatgcccg cgtagcgcac gcagcgggcc acttcctcgg cgtcgaggtt
     4621 gatctgcacg cccaacgctt ccagcacgtc cgcgctgccg cacttgctgc tcatggcgcg
     4681 gttgccgtgc ttggcgatcg gcacgcccgc ggcggcagcc acaatcgcgg cggcggtgga
     4741 gatgttgaag gtgcctgagc ggtcgcctcc cgtgccgcag gtgtctatca cctcaaggtc
     4801 gctcacgtcc accggggtgg cgcggctcct catcacgcga gccaggcccg cgatctcctc
     4861 gaccgtttcg cccttcatgc gcagggcaat cagcagggcc gctatctgcg cgggggtggc
     4921 ctcgccgtcc atgatctccg acatcgcccc cgacgcctgt tcggcatcga gcgactcgcc
     4981 ccgcaccagc ttgtcaatag cttcgagtat catcgctagc tcctttgtgg gtggtcagtg
     5041 gtcagtggtc ggtgcaagtg tcaccttgca ccctctaaac cttattttct ttgcgcgtcc
     5101 tttgcgttct ttgcgtcttt gcgtttccgt ctcttacctg accactgacc accaacaa
//
LOCUS       DASRWZ010000328         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_644764, whole genome shotgun sequence.
ACCESSION   DASRWZ010000328 DASRWZ010000000
VERSION     DASRWZ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644764"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1385
                     /locus_tag="VFH60_05080"
     CDS             120..1385
                     /locus_tag="VFH60_05080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6313187.1"
                     /translation="MQAQARPQLQPPTVETEQADPTKLDVRQLGFLVAGMFVAMLTLT
                     LPVAQLGEFVGRLLPNGGADVELENTTKGLFQTMHFLAYIPFAFVWGALSDRSGKRKP
                     FLMIGMLGQAVMFALMPLIDNLWLLYAARFIEGTFSIAVVSMLMTMTLDIAPPGRRGM
                     TLGVFTMGMLLGNGLGAPIGGALSELSMSYPFWLGAGLLALMLVVISVGVRETSAVTP
                     GYSFRRSMLVFREHPRLGIPYAFTMLDRLTVGFFVGQFPILADVVYGFGPRDRGIYLM
                     VFLLCFALFSPVGGAISDRYGRVKPMLLGTVAYGLMLMLIGRVGPELLYVVMAFGGLC
                     GAILYPPSIALVGDYAGPRQRGVAMGGFNLAGSIGFAAGPLIFNLIADGLGILTPPLV
                     AGLLCILAAAVAAPLMLGRRAQERAMLSS"
     gene            1527..2267
                     /locus_tag="VFH60_05085"
     CDS             1527..2267
                     /locus_tag="VFH60_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET6313188.1"
                     /translation="MIWIGTSGWVYKHWIGCFYPPEMKQRDWLRHYASVFPTVEINRS
                     FYRLPTRENFEAWARQAGFHPGFQFATKASRFLTHIKRLNEPEEPVRALLEAAEGLGA
                     HAGPYLYQMPPRWRANPERLRAFIAHLPPGHKAAFEFRDNSWYGEEILRILGEGGYAL
                     VRAIGGYYTPMEVPDVGPFRYLRVHGGQWGIGLTEGELQYWADRIANDAHNGLETYIY
                     FNNDPECQALYNAYRLRDMLAYTGAVVP"
BASE COUNT          432 a          792 c          729 g          494 t
ORIGIN      
        1 tccccacagg acgatattgg gcagattatg cgataattat agaggccaaa gcgccacagt
       61 cgttatacgc aatcgcaaac tgctctgcga ggccgggcgc aaaaagagag accaacaaga
      121 tgcaagcaca agcgcgacca cagcttcagc cacccaccgt tgagacggag caggcggacc
      181 ccaccaaact cgacgtgagg cagttggggt tcctggtggc gggcatgttc gtcgccatgc
      241 tcacgcttac cttgccggta gcccagctag gggagttcgt cggcaggctc ctgcccaacg
      301 gcggggccga cgtggaactg gagaacacca ccaagggcct cttccagacg atgcacttcc
      361 tggcctacat tcccttcgcc ttcgtctggg gcgcgctctc agaccgcagc ggcaagcgca
      421 agcccttcct catgataggc atgctcggac aggcggtgat gttcgccctg atgcctctca
      481 tcgacaacct ctggctgctc tacgccgccc gcttcatcga gggcacgttc agcatcgcgg
      541 tcgtgtcgat gctgatgact atgaccctcg acatcgcgcc gcccggccgc cggggcatga
      601 cgttgggcgt gttcacgatg ggcatgctgc tgggcaacgg cctgggcgca cctatcggcg
      661 gggcgctgtc cgagctttcc atgtcctacc cgttctggct gggcgctggc ctgctcgcgc
      721 tcatgctggt ggtcatcagt gtgggcgtgc gagagacttc cgccgtgacc cccggctact
      781 cgttccgccg ctcgatgctg gtgttccgcg agcacccccg cctgggcata ccctacgcct
      841 tcacgatgtt agaccggctg acggtcggct tcttcgtggg tcagttcccg atcctggccg
      901 acgtggtgta cggcttcggc cccagggacc ggggtatcta cctgatggtc tttttgctct
      961 gcttcgccct gttctccccg gtgggcgggg ccatctccga caggtacggg cgggtcaaac
     1021 ccatgctgct gggcacggta gcctacgggc tgatgctcat gctgataggt cgggtagggc
     1081 cagagctgct atacgtggtg atggcgttcg gcggcctgtg cggggccatc ctctatccgc
     1141 catcgatagc cctggtgggc gactacgcag gcccgcgcca gcggggcgtg gctatgggcg
     1201 gcttcaacct ggcaggctcc atcggcttcg cggctggccc cctcatcttc aacctcatcg
     1261 ccgacggcct gggcatactc actcccccac tcgtggccgg cctcctctgc atcctcgccg
     1321 ccgcggtcgc cgcccccctc atgcttggcc gcagggcgca ggaacgggcg atgcttagtt
     1381 cttaatgctt agtttttaat tcttaattgc ccggttggcc tatttactgc tttccaggta
     1441 tcgcggactt cacgcggtac gtgttactcg tcacgtgtta ctcgtcacgt gttactcgtc
     1501 actcgtcacg tttcaggagt gcagcgatga tctggatagg cacctctggg tgggtgtaca
     1561 agcattggat cgggtgtttt tatccgccgg agatgaagca gcgcgactgg ctgcggcact
     1621 acgctagcgt ctttcccacc gtggaaatca accgtagctt ctaccggctg cccaccaggg
     1681 agaacttcga ggcgtgggcg cggcaggcgg gcttccaccc cggcttccag ttcgcgacca
     1741 aggcgagccg cttcctcacg cacatcaagc ggctgaacga gccggaggag cctgtcaggg
     1801 cgctgctcga ggccgccgag ggattaggag cgcacgccgg tccgtacctg taccagatgc
     1861 cgccccggtg gagggccaac cctgagaggc tgcgcgcctt tatcgcccat ctaccccccg
     1921 gacacaaagc cgcgtttgag tttcgcgata actcctggta cggcgaggag atactgcgca
     1981 tactgggcga agggggctac gcgctggtgc gggccattgg gggctactac accccgatgg
     2041 aggtgcccga cgtggggccg ttccgctacc tgcgcgtgca cggcggacag tggggcatcg
     2101 gcctcaccga aggcgagctg caatactggg ccgaccgcat cgccaacgac gcccacaacg
     2161 gactcgagac ctacatctac ttcaacaacg accccgaatg ccaggccctc tacaacgcct
     2221 accgcctccg cgacatgctc gcctacacag gcgcggtcgt gccttagctc ccattggctc
     2281 gctctactaa acagcttaag agacgaaaac gcaaagacgc aaagaacgca aagaacgcgc
     2341 agagattcag ataagggtgt tctaaacaaa aggcccagcg tacatcatct tgtaccctgg
     2401 tcctttttgt actctccaaa ccttaatcta ctttgcgcgt tctttgc
//
LOCUS       DASRWZ010000329         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_919212, whole genome shotgun sequence.
ACCESSION   DASRWZ010000329 DASRWZ010000000
VERSION     DASRWZ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919212"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            518..1294
                     /locus_tag="VFH60_05090"
     CDS             518..1294
                     /locus_tag="VFH60_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313189.1"
                     /translation="MNSNNSRVITTIRGLFFTVALSLFMAAFVFASSSSAASQYAPLS
                     KPMAQVSATAGDPSPPVDPVPSQTIPVAQTGTVAATGTVAATGTVAATGTVVATGTVV
                     GVTATAATTGTAVATGTVIPRETPVGTGTVAATGTVAATGTVGAGGTATAGTTGTAVA
                     TGTTMAGGTATTVVSSPTAMATEAAPATATTSVGGGFTEATPTAMTTGGGDVGGVTGG
                     EAPGMPRTGGGDNWLALILLALGAASLAAGVLVRAPQKKR"
     gene            complement(1455..2594)
                     /locus_tag="VFH60_05095"
     CDS             complement(1455..2594)
                     /locus_tag="VFH60_05095"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6313190.1"
                     /translation="MNLRRIGLGLLVSVVCLYFVLRGVQWGEVWRHLSEVDLPLFGLS
                     MLLMLVAYFLMTWRWQHLLDPLEVPMSPSDTGGSLPAPASPGGMRSHVSLLNLYGKML
                     TGYFFNAFFPARAGDLVRAYLLGRKTGLRKTTILATIVIEKAFDGIVLLVLLLLSLVV
                     LPSVGAAGVGGLDPDLLAWVAGAGIVVAIAGLGVFYMFSGQIARFTERVVSRLPLPDR
                     LKRLPVRLIDTFASGMHVFKSPLPLVSAAAISVFVWLAVALMFLAGLMSFRTPFPDQL
                     TGIAGLFFITGLVNLGLLVPALPGNVGTYEALVVAAMAFFRVDKELAVAFALIFHVGQ
                     IVVTLAVGLFAFWAQNMSLAEMRPVEAAAEREAERSLEEIERGTL"
BASE COUNT          566 a          865 c          815 g          439 t
ORIGIN      
        1 gggcaggttg cgcagggtgc cggcggaaga ggcgctcggg gtctcggcac ccggcacgtc
       61 ggtagtgggc atggaaatta ctggctccaa tcagtggtcg catacagcgg cgtggtgccg
      121 caccctacta ttttacccca aatgggaagg cctacgccac tctgaggctg ctccgtcaac
      181 ttacaagagg ccctacgacc cgcccacgcc tcgcaccgct atgtgccggg gtttcataaa
      241 cattggtcac aaaagtctcc catgccacat gaaagtagca gggtggcccg aaaaatgctc
      301 ttcaatgccg aagtcaaagc cctgggtcgg agcaggggca taattgctaa taagggagga
      361 gagctgacac tactgagacg gaagccatac acttacacac gcgcacgagc ctgccgaatg
      421 cgtcacgctc gttgccagga ctatctaccc ggagacaacg gcacgtcatg tggtactcag
      481 cgcagagtta ccgactagat aactgaggag actacttatg aattcaaaca actcacgcgt
      541 aatcacaact attcgagggc ttttctttac ggtcgcactg agcctgttca tggcagcctt
      601 cgtgttcgcc tcctcgtcca gtgcggcttc acagtacgcc cctctgagca agcctatggc
      661 ccaggtgtca gcgaccgcgg gcgacccgtc acccccggtt gaccccgtac cgagccagac
      721 cataccggtc gctcagaccg gcactgtggc tgctacgggc accgtggctg ctacgggtac
      781 cgtcgctgcc accggcacag tggttgccac cggcaccgtc gttggcgtga ccgccaccgc
      841 ggctaccaca ggcaccgcag tagctaccgg cacggtcata cctagggaga cccctgtagg
      901 caccggcacc gttgctgcta caggcaccgt tgcggctacg ggcaccgtcg gcgcgggcgg
      961 aacggccaca gccggcacca ccggcaccgc cgtggcaacc ggcaccacga tggctggagg
     1021 cacggccaca acagtagtga gcagcccgac agctatggct accgaggccg cgcccgcgac
     1081 tgctactacc tctgttggtg gcggcttcac cgaggctacc cccacggcta tgaccacagg
     1141 cggcggcgac gttggcggcg taaccggtgg tgaggctcct ggcatgcctc gcacgggtgg
     1201 cggcgacaac tggctggcgc tcatcctcct ggccctcggc gcggcaagcc tcgccgccgg
     1261 cgttctggta cgcgcaccgc agaagaagcg ataactacta agcgcaaccc gccaagtagg
     1321 ccgtaacggt ctacgcggcc aaacacagag acccccctgt acctccacag gggggtctct
     1381 gtgtatttta gattttagat tttagatttt ggattgctag aaattcttct aaatccaaaa
     1441 tccaaaatct aaaatcacaa agtgcctctt tctatctcct ccaggctgcg ctcggcttcg
     1501 cgctcggcgg cggcttcgac ggggcgcatc tcggccaggg acatgttctg cgcccagaag
     1561 gcgaacaggc cgaccgcgag cgtgacgaca atctgcccca cgtggaagat gagcgcgaag
     1621 gccacggcta gctctttgtc cacccggaag aaggccatgg cggccaccac cagcgcctcg
     1681 tacgtgccta cgttgccggg cagcgcgggc accagcagcc ccaggttgac cagccccgta
     1741 atgaagaaga ggcccgctat gccggtgagt tggtccggga acggggtacg aaacgacatc
     1801 agccccgcca ggaacatcag ggcgacggcg agccacacga acaccgagat ggcggccgcg
     1861 ctgaccaggg gcaaggggct tttgaatacg tgcatgccgc tcgcgaaggt gtctatcagg
     1921 cgcaccggca ggcgcttcag gcggtcgggc aggggcagcc tcgacacgac ccgctcggtg
     1981 aagcgcgcta tctgcccgct gaacatgtag aacacgccca gccccgcgat tgccaccact
     2041 attcccgccc ccgctaccca ggccagcagg tcggggtcca gaccgcccac ccccgccgcg
     2101 cccaccgacg gcagcaccac caggctcaac agcaacagca ccagcagcac tatgccgtcg
     2161 aaggccttct ctatcacgat ggtagccagg atggtggtct tgcgcaagcc ggtcttgcgg
     2221 cctaagaggt aggcgcgcac gaggtcgccc gcacgcgctg ggaagaatgc gttgaagaag
     2281 tagcccgtga gcatcttgcc gtacagatta agcaggctta cgtgcgagcg catgcctccg
     2341 ggggaggcgg gtgcggggag gctgccgcca gtatcagacg gggacattgg cacttcgagc
     2401 ggatcgagca ggtgctgcca gcgccaggtc atgaggaaat aggccacgag cataagcagc
     2461 atcgacagcc cgaacagcgg caggtccacc tcgctaaggt gccgccacac ctcgccccac
     2521 tgcaccccgc gcaacacgaa gtagaggcag actacgctga caagcaggcc gaggccgatt
     2581 ctgcggaggt tcattggaag cgatgagtga cgagtgacga gtgacgagtg acgagtgacg
     2641 agtaacgggg tttgtttgcc agctcctgcg tatcactcgt cactc
//
LOCUS       DASRWZ010000330         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_644925, whole genome shotgun sequence.
ACCESSION   DASRWZ010000330 DASRWZ010000000
VERSION     DASRWZ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644925"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1555
                     /locus_tag="VFH60_05100"
     CDS             185..1555
                     /locus_tag="VFH60_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6313191.1"
                     /translation="MSEGTDFGAYDAYIESNFEDMLKELRRFCSQPTLAGQGIGLVEG
                     VALVRESLEPLGARVKVVPIEGAPPVVLAELGGGERTLLLYNHYDVQPPEPLELWDSP
                     PYAGEVRDGRFYARGAADDRGDLLSRILAIRAYQATIGDLPLRIRWLVEGEEEVGSPN
                     LGPVALEHADDLRADWCAWEGSWRDINDNPQVICGLKGMLYVELHATGASYDIHSGLG
                     GIIPNPAWRLVLALGTLRDERGNFVMDGLEDLVDRPTQAEVDAIHRMPFDEQAWLKQY
                     GIKGWQRGLGGHEVLVVEQFEPTANIAGFVSGYGGEGSKTIVPSKAMVKMDFRLVPHQ
                     TPEKMLALLRAHLDRRGYDDIEIVKLGELLPAKTPIDSPLVKVSEQVWHDLGEEQVVI
                     APMTGGSGPASLIANELGIPLVITAGVSFSGSRAHAPNESIRLDHFKQGIRYWGRFFA
                     RLAAME"
     gene            1737..>2031
                     /locus_tag="VFH60_05105"
     CDS             1737..>2031
                     /locus_tag="VFH60_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6313192.1"
                     /translation="MSNFPLRIDIKTAQHDRDWDNMLRYWLDAEAEEAFTGAWLFDHF
                     VPINGHVEGPCMDGWTLLAALAAATKRLRLGLMVGCNGYRHPAVLAKIATTIDR"
BASE COUNT          373 a          648 c          651 g          359 t
ORIGIN      
        1 accctggtcc tccttgtact ctccacccta tcctaaactt tgcgcgttct ttgcgacctt
       61 tgcgtctttg cgttttcgta ctcttggcac gctcttggcg tcttggcgtt tccgtctatt
      121 tgagtcgctt tgacgattat ctactttcgt gttaggctgc cctattacgt gcaaggaggc
      181 agccatgtcg gaggggaccg atttcggggc gtacgacgct tatatagaga gtaacttcga
      241 ggatatgctc aaggagttgc ggcgtttctg ctcgcagccg acgctggcgg gacagggcat
      301 cgggctggta gagggagtgg cgctggtgcg ggagtcgttg gagccgctgg gcgcacgggt
      361 caaggtggtg ccgattgagg gcgcgccgcc ggtggtgctg gccgagcttg gcggcgggga
      421 gcgcaccctg ctcctgtaca accactacga cgtgcagccc cccgagccgc tggagctttg
      481 ggactcgccg ccctacgccg gggaggtgcg cgacggccgg ttctacgcac gcggcgcggc
      541 cgacgaccgg ggcgacctcc tctcgcgcat actcgccatc cgcgcctacc aggccacgat
      601 aggcgacctg cccctccgca tccgctggct cgtggagggc gaggaagagg tgggcagccc
      661 caatctcggc cccgtcgccc tggaacacgc agatgacttg agggccgact ggtgcgcctg
      721 ggaaggctcg tggcgcgaca taaacgacaa cccgcaggtg atatgcggcc tcaagggcat
      781 gctctacgtc gagctacacg ccaccggggc ctcctacgac atccattcgg gcctgggcgg
      841 tatcataccc aacccggcgt ggcggctcgt gctcgccctc ggcacgttgc gagacgagcg
      901 cggcaacttc gtcatggacg gcctggaaga cctggtggac cgccccacgc aagccgaagt
      961 agacgccatc caccgcatgc ccttcgacga gcaggcgtgg ctcaaacagt acggcatcaa
     1021 gggctggcag cgcggcttgg gcggccacga agtgctcgtc gtggagcagt tcgagccgac
     1081 cgccaacatc gcgggcttcg tgagcggcta cggcggtgag gggtccaaga ccatcgtgcc
     1141 gagcaaggca atggtcaaga tggacttccg cctggtgccc catcagaccc ccgaaaaaat
     1201 gctggccctg ctgcgcgccc acctcgacag gcgcggctac gacgacatag agatagtcaa
     1261 gctgggcgag ctactacccg ccaaaactcc catcgacagc ccgctggtga aggtctcgga
     1321 gcaagtgtgg cacgacctgg gggaggagca ggtggtaata gcccccatga ccggtggcag
     1381 cggcccggcc tccctcatcg ccaacgagct aggcataccg ctggtcatca ccgccggtgt
     1441 aagcttctcc ggctcccgcg cccacgcccc caacgaatcg atccgcctgg accacttcaa
     1501 gcaaggcatc cgctactggg gccgcttctt cgcccggctg gcggcgatgg agtaatgagt
     1561 gccgagtgcc gagtgccgag tgccgagtat tgagacgaaa acgcaaagac gcaaagaacg
     1621 caaaggacgc gcaaagaaga ttgaggtttt ggcgtcgtgc cactgaccac tgaccactga
     1681 ccactgacca ctcggcactc ggcattcggc actcttcacc tatggagtct gcaactatga
     1741 gcaatttccc gcttcgtatc gacatcaaga ccgcccagca cgaccgcgac tgggacaaca
     1801 tgctgcgcta ctggctggac gcggaagcgg aggaggcgtt cacgggcgca tggctgttcg
     1861 accattttgt gcctatcaac gggcacgtag aggggccgtg catggacggg tggacgctgc
     1921 ttgccgccct cgcggcagcc acgaagcggt tgcggctcgg cctgatggtg ggctgcaacg
     1981 gctaccgcca ccccgccgtg ctcgccaaga tcgctaccac gattgaccgg g
//
LOCUS       DASRWZ010000331         4282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_27960,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000331 DASRWZ010000000
VERSION     DASRWZ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27960"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFH60_05110"
     CDS             <1..508
                     /locus_tag="VFH60_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313193.1"
                     /translation="LRSLLLLAGLVQLVGCTSPAPSPTPPISHTPTGVAASSQGSKFA
                     DYSYRLDVSSLVAQDWHPGDSLPLQWEALDAYGDAIGPAVPLTLTVTLTGPAASKEEA
                     VQQLRSGNQEVASNVILIDNATSKVPANELRLPGDLLPGMYSLTQTIETASSKEEATT
                     TISVVSKP"
     gene            505..1143
                     /gene="pdxT"
                     /locus_tag="VFH60_05115"
     CDS             505..1143
                     /gene="pdxT"
                     /locus_tag="VFH60_05115"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET6313194.1"
                     /translation="MKIGVLALQGAFREHIETLRKLGAEAVEVRLPGQLEGLDGLIIP
                     GGESTAIGKLAVKYGLQEAIQRFATSGKPVYGTCAGMILISKDVGRDQPLLGLMDVQV
                     ARNAFGRQIDSFETDLPMPCLGDAPFPAIFIRAPKIAGVGEGVEVLASLPDGTPVAAR
                     EGQMLVTSFHPELSDDLRLHRYFLDMVVASLQAQSPGPSQPESGTKTQLMKA"
     gene            1186..2268
                     /locus_tag="VFH60_05120"
     CDS             1186..2268
                     /locus_tag="VFH60_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313195.1"
                     /translation="MTIIQDSTLKTQNSTRGFVREATPLDLLRIVATRRRQRTLRLNP
                     PYSLIQPQSILPELLRSQVPFRLRAGCVLVYVEKGTVHGFVQARPRWGREDEWAVTTL
                     ATTDRAPYHVSEALLEAVSQAAGEYGVLRLFVKVPSDESDLPQFRRIGFTHYTNEHIW
                     GHLYFAQRGQGTSADEPSHAALRKQSNADAWDLLQLYSAVTPPAVQRAETLSTRNFRR
                     NYLPRPLLFSSGLVERAYVWPDASGQKQALGGYIRLVTGARAHGLTTMFRPDGANRDV
                     CPQAFDYVLWKAARSGPKPVYCGVRDYQAELSGILEARGFYHFSEQALLVKYLAEPIK
                     VKQPVLAPFLARAPGELVTTAKASNE"
     gene            2532..>4282
                     /locus_tag="VFH60_05125"
     CDS             2532..>4282
                     /locus_tag="VFH60_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6313196.1"
                     /translation="MHENLARGSYEGLEADLPKIDRAPTAAPAQALAEEHKTTENPNE
                     VVRNGGEHVVTDDLEALIAALPPHIIQPIREYNDRVNLLEVVMDLGRTPEARYRNKER
                     TLSESEVSRADLDYVTERIGEFGGDNRAGIERTLHRISCIRNRRGEVVGLTCRIGRAV
                     YGTISIIKDLVETGQSILMVGPPGVGKTTLLRECARVLSDEVRKRVVIVDTSNEIAGD
                     GDIPHPSIGRARRMQVPLPERQHAVMIEAVENHMPEVIIIDEIGTELEAQAARTIAER
                     GVQLVGTAHGTSLENLMLNPTLSDLVGGIQTVTLGDEEARRRGTQKSVLERKAPPTFD
                     IMVEILDRERVAVYRDVAEMVDSLLRGDILPPEIRTRKADGTIHTEIAHITPNRERAG
                     SPSMRREQQQTRGGRYNERGYGSHGNFDQGPPNAPRRQMQGGQSGAMRGERLTGRNFQ
                     DGGGGGRNRPFESGRVDTGNATPSNVGFGAPMASTQAAAYDENDLVDVRQEEGRGEGS
                     PDGNREAAREASRTTRIGGSASSNKPLRIYPFGVSRNRLEQAIKDLRVPSTMVRDVDN
                     ADIVMTLKNYYRKSPQM"
BASE COUNT          909 a         1429 c         1285 g          659 t
ORIGIN      
        1 actacgtagt ctcttgctcc tggcggggct tgtgcagctt gtgggttgta caagtcccgc
       61 gccttcacct acgcctccga tcagccacac acccaccggc gtagctgctt cctctcaagg
      121 atccaagttt gcagattaca gctatcgact tgatgttagc tcgcttgtgg cgcaagattg
      181 gcaccccggc gatagtctgc cgctacagtg ggaagcgctg gatgcatacg gggacgctat
      241 cggacccgct gtcccactga cgctgactgt aacgctgacc ggaccggctg cctctaaaga
      301 agaagcagtc cagcagctaa gaagcggcaa ccaggaagtg gcgtccaacg taattctcat
      361 agacaacgcg acttccaaag taccggcgaa cgaactaagg ctacccggcg acctgctccc
      421 cggtatgtac tcactcacgc agaccataga aacagcaagc tcgaaggaag aggcaacaac
      481 aacgataagc gtggttagca agccatgaaa ataggcgtac tggcccttca gggcgcgttc
      541 agagagcaca tagagaccct tcgcaagctg ggcgctgagg ccgtcgaggt ccgcctgccg
      601 gggcagctag aggggcttga cggcctcatc atacccggcg gcgagagcac ggcgataggc
      661 aagctggcgg tcaagtacgg cctgcaagag gccatacaac ggttcgcaac gtcaggcaag
      721 ccggtgtatg gcacctgcgc gggcatgatc ctcatctcga aggacgtggg gcgcgaccag
      781 cctctcctcg gcctcatgga cgtgcaggtg gcgcgcaacg ccttcggccg ccagatcgat
      841 agcttcgaga ctgaccttcc gatgccctgc ctgggtgatg cgcccttccc cgccatcttc
      901 atccgcgccc ccaagatcgc cggcgtgggc gaaggcgtgg aggtactcgc ctcactcccc
      961 gacggcacgc ccgtggccgc tcgcgagggc cagatgcttg tgacctcctt ccaccccgag
     1021 ctgagcgacg acctgcgcct ccaccgctac ttcctcgaca tggtagtggc cagcctgcaa
     1081 gcccaatcgc ccggaccttc ccaaccggaa agcggcacga agacgcagtt aatgaaagcg
     1141 tagttttgaa ttaccccgcc ggatgtatta cactgccggg agaaggtgac cataattcaa
     1201 gactcgacac tcaaaactca aaactccacc agaggctttg tacgcgaagc cacgcccctg
     1261 gacctgctgc gaatcgtcgc cacgcgccgc cgccagagga ccctgcgcct caaccccccc
     1321 tacagcctga tacagcccca gtcgattttg ccggagctat tgcgctccca ggtgccattt
     1381 aggcttcgcg ccggttgcgt gctcgtatac gtcgagaagg gcacggtgca cggcttcgtg
     1441 caggcccgac cccgctgggg ccgcgaggac gagtgggccg tcaccacgct cgccaccacc
     1501 gaccgcgccc cctaccacgt gtccgaggcg ttgctggaag cggtgtcgca ggcggcgggc
     1561 gagtacgggg tgctgcgcct cttcgtgaag gtgcccagcg acgagtcgga cctcccgcag
     1621 ttccgccgca tcggcttcac ccactacacc aacgagcaca tctgggggca cctctacttc
     1681 gcccaacgcg ggcagggcac ctccgccgac gagccgagcc acgccgccct ccgcaagcag
     1741 agcaacgccg acgcctggga cctcctgcaa ctctactcgg ccgtgacgcc cccagccgtg
     1801 cagcgcgcag agacgctcag cacccgcaac ttccgccgca actacctgcc tcgccccctg
     1861 ctcttctcgt ccggcctggt ggagcgggcc tacgtctggc ccgacgcgag cgggcagaag
     1921 caggcgctgg gaggctacat ccggctggtg accggggcgc gcgcccacgg gctcaccacc
     1981 atgttccgcc ccgacggcgc gaacagggac gtgtgccccc aggccttcga ctacgtgctg
     2041 tggaaagccg ccaggtccgg ccccaaaccg gtgtattgtg gcgtcagaga ttaccaggcg
     2101 gaactaagcg gcatattgga agccaggggc ttctatcact tcagcgagca agccctactc
     2161 gtgaagtacc ttgccgaacc gataaaagtc aagcagccag tcctcgcccc cttcctggcc
     2221 cgagcgccgg gcgagctggt caccacggcg aaagcaagta atgagtaatg aggactgaga
     2281 cgagtaatga gtgatgagga ctgaggactg agggttaata cgttcgcagg accgaggttt
     2341 cagaacttgc tctatgctaa catgtacagt gggaggatat ggcgaaagaa ctgggcaaac
     2401 caaactactc agtcctcagt cctcatcact cattactcat ctgtggcact aactttgcac
     2461 atatagtggc taagacgcca ccgggaccat ttgggaccgg tttgtaaccc aaaaggggtg
     2521 atctcttaac aatgcatgaa aatctagctc gtggtagcta tgaaggtctc gaagcagacc
     2581 tgcccaagat cgaccgtgcc cctaccgctg ccccagccca ggccctggcg gaggagcaca
     2641 agactactga aaaccccaat gaagtagtcc gtaatggcgg cgagcacgtc gtcaccgacg
     2701 accttgaagc cctcatagcg gccctcccac ctcacataat tcagcccatc agggaataca
     2761 acgaccgcgt caacctcctc gaagtggtca tggacctggg ccgcacgccc gaggcccgct
     2821 accgcaacaa ggagcgcacg ctcagcgagt ccgaggtcag ccgcgccgac ctggactacg
     2881 taaccgagcg catcggggag ttcggcggcg acaaccgcgc gggcatcgag cgcaccctgc
     2941 accgcatctc ctgcatccgc aaccgcagag gcgaggtggt gggcctcacc tgccgcatag
     3001 gccgcgcggt ctacggcacc atctccatca tcaaggacct ggtggagacg ggccagagca
     3061 tcctcatggt ggggcctccc ggcgtgggta agaccaccct gctgcgcgag tgcgcccgcg
     3121 tgctctccga cgaagtgcgc aagcgcgtgg tgatcgtgga cacctccaac gaaatcgcgg
     3181 gcgacggcga catcccccac ccctccatcg gcagggcgcg caggatgcag gtgccgctgc
     3241 ccgaacgcca gcacgccgtg atgatcgagg cggtcgagaa ccacatgcct gaagtgatca
     3301 tcatcgatga gataggcacc gagcttgagg cccaggccgc ccgcaccata gccgagcgcg
     3361 gcgtgcagct tgtgggcacc gcccacggca ccagcctcga aaacctgatg ctcaacccca
     3421 ccctctccga cctcgtcggc ggcatccaga ccgtgacgct cggcgacgaa gaggccaggc
     3481 ggcgcggcac ccagaagtcg gtgctggagc gcaaggcccc gcccaccttc gacatcatgg
     3541 tggagatact cgacagggag cgcgtggcgg tgtaccgcga cgtagccgag atggtcgact
     3601 cgctgttgcg cggcgacata ctcccgcctg agatacgcac ccgcaaggcc gatggcacaa
     3661 tccataccga gatcgcccac atcacaccca accgcgagcg tgcgggcagc ccgtctatgc
     3721 gccgcgaaca gcagcagacg aggggcggcc gctacaacga gcgcggctac ggctcccacg
     3781 gcaacttcga ccaggggcca cccaacgcgc ccaggcggca gatgcagggt gggcagtccg
     3841 gcgcgatgag aggcgagcgg ctgacgggcc gcaacttcca ggacggcggt ggaggcggga
     3901 gaaatcgccc ttttgagagt gggcgcgttg ataccggaaa cgcaacaccg tcgaacgtag
     3961 ggttcggcgc gcccatggct tccacacagg cggcagctta cgacgagaac gaccttgtag
     4021 acgtgcggca agaggaaggt cgcggcgaag gcagccccga cggaaacagg gaagccgccc
     4081 gcgaggccag caggaccacc cgcatcgggg gcagcgcctc ctccaacaag ccgctgcgta
     4141 tctacccgtt cggggtgagc cgcaacaggc tggagcaggc gatcaaggac ctgcgagtgc
     4201 ccagcacgat ggtgcgcgac gttgacaacg ccgacatcgt gatgacgctc aagaactact
     4261 accgcaaaag tccgcaaatg tt
//
LOCUS       DASRWZ010000332         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_713533, whole genome shotgun sequence.
ACCESSION   DASRWZ010000332 DASRWZ010000000
VERSION     DASRWZ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713533"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFH60_05130"
     CDS             <1..536
                     /locus_tag="VFH60_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313197.1"
                     /translation="YLPWGIYKVEPGAPPAATLPFSVNVGEMDYPWLVAGFNKQETSD
                     GGASYWRWTGNHAILRLPVPVAPGGASYGGARVTVRMRPETRVEGASILRMDPLTITV
                     ALDDTQIGQVVLAPGSDFQDYTFTVPPGTPKKERDPTTGLLHVYSPTWSGSAGGLGNE
                     RRALGVQLDAVRLEALP"
     gene            616..2211
                     /locus_tag="VFH60_05135"
     CDS             616..2211
                     /locus_tag="VFH60_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HET6313198.1"
                     /translation="MSRDTRPLVERAGLAAFWNAAFFPLKMLVAFGASVITVRLLRNE
                     GWALYLVVASLLNWFGLVVDLGIERTLPRFYPEIEMRYGKRGIMRLLLWVSVIKGAVL
                     LALLLALVAVPDFWISTFALGEQGGLFLVMIAVLLVLGALSDVTIQLLYTHFRQKVTN
                     SLDVLASIGFPVLSSIFVYMELGAMGMLLALLITTVVSVALSTWQAWVMLASMDPEPP
                     LKSADVKLPSTRSLRDRIGGFAGLNYLINWTVNLYDLPFFTIMLSLIVVDQAARAVQI
                     SILGLAYRVTRQFLRALVVPLTGVQTPLFARLYAEGRMDGLRTAYATLTKFLILALLP
                     AGVGLIVSARNLLAVFFVQIGPDAVLTWPKVPDAVACIAILTIGLFGEAIISVVLNVL
                     MVHEDYRAVITARLVALCSIPLVLVLVPLMGVTGAAIAASVAALASRAVALVYAVRRV
                     GLRFPGEFFVRVGLASSIMGAALLPFLVFFPPTPLPTAAMILTGALIFYVMFRVLGGM
                     DQDDKDRFSTLRVPFVRVVLRYL"
BASE COUNT          336 a          694 c          724 g          477 t
ORIGIN      
        1 cgtacctgcc gtggggcatc tacaaggtcg agccgggtgc gcccccggcg gctactctgc
       61 ctttctccgt gaatgtcggg gagatggact acccctggct ggtggccggg ttcaacaagc
      121 aggagacttc cgacgggggc gcgagctact ggcggtggac gggcaaccac gccatcttgc
      181 gcctgcccgt acctgtagca cccggcggcg cgtcttacgg aggggcgcgc gtcaccgttc
      241 gcatgcggcc tgaaacgcgt gtcgaggggg catctattct caggatggac cccttgacaa
      301 ttaccgtggc tttggacgac actcagatag gccaggtggt gctcgcgccg ggcagcgact
      361 tccaggacta caccttcacc gtgccgcccg gtacgcccaa gaaggagcgc gaccccacga
      421 ccggcctgct acacgtttac tctcccacct ggtccggcag cgcgggcggc ctcggcaatg
      481 aacgccgcgc tctgggggtg cagctcgatg ccgtgcgact ggaagcccta ccgtgatacc
      541 cgttagtagc ttgtacgtgg tacgcattac gagcgcggtg gtgtcatcgc gccccaaatc
      601 cgcaggcagc acccattgag cagggacacc agaccactag tcgaacgagc ggggcttgca
      661 gccttctgga acgctgcatt cttcccattg aaaatgctgg tggcgtttgg agccagcgtc
      721 atcaccgtgc gcctgctgag gaacgagggc tgggcgctct acctggtggt cgcgtcgctg
      781 ctcaactggt tcgggctggt ggtggacctg ggcatagagc ggacgctgcc ccgtttctat
      841 cccgaaatcg agatgcgcta tggcaagcgt ggcatcatgc ggctgctcct gtgggttagc
      901 gtgataaagg gcgcggtgct tctagctttg ctcctggcgc tggtggcggt gcccgatttc
      961 tggatttcga cgtttgccct gggggagcaa ggcgggttgt tcctggtaat gatagccgtg
     1021 ctgctggtgc tcggcgcgtt gtccgacgtg acgatccagc tactttacac gcacttccgc
     1081 cagaaggtta ccaactccct cgacgtactg gcaagcatcg gcttcccggt gcttagctct
     1141 atcttcgtct acatggagct gggcgcaatg ggtatgttgc tcgccctgct catcaccacg
     1201 gttgtctcag tggcgctgtc aacctggcag gcgtgggtga tgctggcgag catggacccc
     1261 gaaccgccct tgaagtcggc cgacgtgaag ctcccctcca cgcgctcact gcgcgaccgc
     1321 atcgggggct tcgcgggcct caattacctc atcaactgga cggtcaactt gtacgacctg
     1381 cctttcttca cgatcatgct cagcctcatc gtcgtggacc aggccgcgag ggccgtgcag
     1441 atttccatac tcggtctggc ctaccgcgtg acaaggcagt tccttcgcgc cctcgtggtg
     1501 cccctgacgg gggtgcagac gcccttgttc gcccgcctgt atgcggaggg ccgcatggac
     1561 ggactgcgca ccgcctacgc cacgctcacc aagttcctga tactggctct gctccccgcg
     1621 ggcgtgggcc tgatagtcag cgcgcgcaac ttgctggcgg tgttcttcgt gcagattggc
     1681 cccgatgcgg tactcacctg gccgaaagtg ccggacgcgg tggcctgcat cgctatactt
     1741 acgatagggt tattcggtga ggcgattatc agcgtggtac tgaacgtgtt gatggtgcac
     1801 gaagactacc gggcggtcat cacggcgcga ttggtggcgc tttgcagcat cccgctggtg
     1861 cttgtgctgg taccgctcat gggggtcacg ggcgcggcga tagcggcctc ggtggcggcg
     1921 ctggcgtcgc gggcggtggc cctggtgtac gcggtgcggc gggtagggct gagattcccc
     1981 ggcgagttct tcgtgcgcgt gggtctcgcc tcctcaatca tgggagccgc cctgctgccg
     2041 ttcctggtct tcttcccgcc cacgcccctg cccaccgccg ccatgattct cacgggcgcg
     2101 cttatcttct acgtgatgtt cagggtgctt gggggtatgg accaggacga caaggaccgc
     2161 ttttccactc tgcgcgtgcc cttcgtgcgc gtcgtgttga ggtacctgta gttgcgggta
     2221 gccatcgtca c
//
LOCUS       DASRWZ010000333         6376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1193499, whole genome shotgun sequence.
ACCESSION   DASRWZ010000333 DASRWZ010000000
VERSION     DASRWZ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193499"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2622
                     /locus_tag="VFH60_05140"
     CDS             <1..2622
                     /locus_tag="VFH60_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313199.1"
                     /translation="KAVDNVLDVIGPELVGYDASEQRLVDQRLLDILERDEDFRCFVL
                     DGQTIMVDDYLEVRPEAAERVLRMVSQGRIKIGPWYTAVDTFLPDPESLVRNLHIGSW
                     SARRHGSDPMPVGHLPDTFGFIGQMPQLLRHFGLQYAFAWRGFHPENEGGTFWWASPD
                     GSRVLTMRPLEGYCEGAMGVVNPERFLAEILPGIAEKQDREPHPHRLFVVGCDHFTAS
                     PRLPWLAERIGEQLGLPARVGSLEEMGALLDPFGDRIPTVRGEQRDPCLAVCPASVCG
                     TRVPLKQANQQVEALLLGVAEPLQALSELAGGGSDRAHLRWAWQILTQNHPHDSIPGC
                     STDEVHREMFTRFARAHRVAIDAIQRGAKRVAASLDPSVLGELGAIGIVGLTGGVGRL
                     RLRLHGPDTGMPRFRIVEPGGREVPYVVVRRGKEHVYYHRLEDAFATMAKTAYAHFAA
                     PRAWVDERRTHPETYYLPYADIEMEVEAPRAGYKILKIEPLKGRQQPGARFTDHDAAS
                     RPEISDGRLRVWADERGLYVEHQASGRSYGPVYFSHAGEAGDEYTAFPVPGEPPVLFA
                     PNAKKAKVATDGLGSLLEMPIGVTVPARLRADRKGRTGRARLTGKMSVRLLGGRVEID
                     LSLLNRARDYDLRLIAHVPGADTARAGAPFNVEDRAFEVDHSSPKAPQQMLPDFPFRG
                     WVAAQDADGAGLAVLARGLYESAVRKVEGGVDVAMTLLRGVGHLSRDDLDTRPDHAGP
                     YIATPDAQCLGPQQWGLALLPFGPGEADSIPVQCEQFLRPPATFPVQWVAGSAPAERN
                     LFAGDDLLVVSALKPSESGEGATLHAHNPTRAERSAEVNGTRVRLDETPVEGDGTLSP
                     FAIAAWRLNGDWKLSSE"
     gene            2951..5299
                     /locus_tag="VFH60_05145"
     CDS             2951..5299
                     /locus_tag="VFH60_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET6313200.1"
                     /translation="MSGPPGPLTGPGQRGGNSIERLPPHWRLRDDNPNEWIWTPRAQR
                     RHDLSPWLEVSVPMSVQEALWRAGQLPHPYRDLNSRLAEWVEHRDWVYGLDFPLPPPP
                     PGTRVFIEFESVADACMLFLNGTLVGEHEGPGAPFHFEIGPALREDSNQLLLVVRAPH
                     QEDPQTGWTERTTSLRGRMGFGWDFAPRLVALGILGDVQLRHTGPWKLRDLWARPALS
                     EDLLRSTIDLSVQVDGPPAAGVRFTISRGRRTVAVETATVGSDGLARATIELVRPDLW
                     WPNDMGGQPPYNVRAECVDGSDAVSSTFGIRTIAWEGRPGGSPAEWPLTLAVNGRRVF
                     QRGWNWVPADSMGGPRAAPLARKLLRLAKEAHVNVLRCWGGADPETRAFYDECDRLGL
                     LVWQEFALSSAGISNVPPKDPAYLDRLEAYARDVVAARRNHPSLALWGGGNELTGDDG
                     KPLAMDHPYPQRLAAVIAAHDPVRAFRPSSPLGPAFDADPEHGNAYLWDVHGPWEYSQ
                     RWPGPQYWRFNAVRPILHSETGAPGEASLAMQRHYLSPSHRAYRLPTNPARRHHGGAW
                     WDHSSTVEQLFGPIEDEELAVLASQWLQAETLRYYIEETRRRWPESVGIYPWQLNEPW
                     PNVACTSAVEYGGRPKLAYFAVRGAYRPLLPTAHYRGLPFSQAEPLHAEIWALNDGPA
                     LEAELEVSFSGLDGVELAPGITHAVRLVASSSTRLMDVAPSLPGDFEGVVIMDLALRG
                     ARNRYIFSSRQEHPLRDTLRYHQLLKSMFVTQPDAPAQPIRG"
     gene            5302..6222
                     /locus_tag="VFH60_05150"
     CDS             5302..6222
                     /locus_tag="VFH60_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET6313201.1"
                     /translation="MSTHTRPSNVITLREILTAAERGGYGIGSFSPRYTPMILPVLRA
                     GQKMRSPLIVQISNNELRRYTITAGEFADEFYRVLRDEQITVPVVLHLDHTKELGVIR
                     EAIAAGFTSVMMDASEKPLEDNIAASREAAEYAHAHGASAEAELGMIGTTDKIETDTD
                     EELYTDPLEAERFVRETGVDALAVSVGTAHGVYMVRQPRIDYDRLKSIKSLTGVHMVL
                     HGGSGVPPEMMWQAITLPGGGVSKVNIATDLEVALLEALGREERMTNAECRALPPDPL
                     ARGRAAVERTVCDKMENFLHSAGKAEGFTF"
BASE COUNT         1089 a         2227 c         2040 g         1020 t
ORIGIN      
        1 aaggccgtcg acaacgtgct cgacgtcatc ggccccgagc tggtgggcta cgacgccagt
       61 gagcagcgcc tggtcgacca gcggctgctc gacatactgg agcgcgacga ggacttccgg
      121 tgcttcgtgc tcgacggcca gacgatcatg gtggacgact acctcgaagt gcgccccgaa
      181 gccgccgagc gggtgcttcg catggtcagc cagggccgca tcaagatagg cccctggtac
      241 actgctgtgg acaccttcct ccccgacccc gagtcgctcg tgcgcaacct gcatatcggc
      301 tcctggtccg cccgccgcca cgggtccgat ccgatgccgg tgggccacct ccccgacacg
      361 ttcggcttca taggccagat gccccagcta ctgcgccact tcggcctcca gtacgccttc
      421 gcctggcgtg gtttccaccc cgaaaacgag ggcggcacct tctggtgggc ctcccccgac
      481 ggcagccgcg tgctcaccat gcgtcccctc gagggctact gcgagggcgc gatgggcgtg
      541 gtaaaccccg aacgcttcct tgccgagata ctccccggca tcgcggagaa gcaagaccgc
      601 gagccgcacc cccacagact cttcgtggtg ggctgcgacc acttcaccgc cagcccccgc
      661 ctgccctggc ttgccgagcg cattggggag caactgggcc tccccgcccg cgttggctct
      721 ctcgaggaga tgggggcgtt gctcgacccg ttcggggacc gcataccaac cgtcagggga
      781 gagcagcgcg acccctgcct ggcggtgtgc cctgcctctg tctgcggtac ccgcgtgccg
      841 ctgaagcagg ccaaccagca ggtcgaggcg ttgctgctcg gtgtggccga gccgttgcag
      901 gccctgtccg aactggcggg gggcggctcc gaccgcgcac acctgcgctg ggcctggcag
      961 attctcaccc agaaccaccc tcacgactcc atccccggtt gcagcactga cgaagtgcac
     1021 cgggagatgt tcacccgctt cgcccgcgcc cacagggtcg ccatagatgc gatccagcgt
     1081 ggggccaagc gtgttgccgc ctccctcgat ccgtccgtgc tgggcgagtt gggggcaatc
     1141 ggtatcgtgg ggcttaccgg gggcgtgggc aggttgcgct tgcgactgca cggccccgac
     1201 accggcatgc ctcgcttccg catcgtggag cctgggggcc gcgaagtacc ctacgtggtc
     1261 gtgcgccggg gcaaggagca cgtctactac caccgcctag aggacgcttt cgccacgatg
     1321 gccaagaccg cctacgccca cttcgccgcg ccccgcgcct gggtggacga gcggcgcacg
     1381 caccctgaga cgtactacct gccctacgcc gacatcgaga tggaggtgga agccccacgc
     1441 gccgggtaca aaatactcaa gatcgagccg ctgaagggca gacagcagcc gggagcgcgc
     1501 ttcaccgacc atgatgccgc ctcccgcccg gaaatctccg acggccggct acgggtatgg
     1561 gcggacgagc gcggcctgta cgtggaacac caggcttcgg gcagaagcta cggtcccgtc
     1621 tacttctccc acgcgggaga ggcgggcgac gagtacaccg ccttccccgt gcctggcgag
     1681 ccgcccgtgc tgttcgcccc caacgcaaag aaagctaagg tcgccaccga cggcctgggc
     1741 agcctgctgg agatgcccat cggcgtcacc gtgcccgccc gcctgcgcgc cgaccggaaa
     1801 ggccgcaccg ggcgcgcccg gctgaccggc aagatgtcgg tgcgcctcct cggcggtagg
     1861 gtggagatag acctgtcgct cctgaacagg gcgcgcgact acgacctgcg cctcatcgcc
     1921 cacgtgccgg gtgccgatac ggcccgcgcc ggtgcgcctt tcaacgtgga ggaccgcgcc
     1981 ttcgaggtgg accattcgtc ccccaaagcc ccccagcaga tgctgcccga cttccccttc
     2041 agggggtggg tggccgccca ggacgcggac ggggccgggc tggcggtgct ggcgcggggc
     2101 ctgtacgaat cggcggtgcg caaggtggaa gggggcgtag acgtagctat gacgctcctg
     2161 cgtggcgtcg gccacctctc gcgggacgac ctcgataccc gcccggacca cgccggcccc
     2221 tacatagcca cgcccgatgc ccagtgcctc ggcccgcagc agtggggcct ggcgctcctg
     2281 cccttcgggc cgggtgaagc cgactccatc ccggtacaat gcgagcagtt cctgcgcccg
     2341 cccgccacct tcccggtgca gtgggtggcc ggtagcgccc ccgccgagcg caacctcttc
     2401 gcgggtgacg acctgctggt ggtgagcgcc ctcaagccct ccgagagcgg cgagggtgcc
     2461 actctgcacg cccacaaccc cacccgcgcc gagcggtccg cagaggtcaa cggcaccagg
     2521 gtacgcctcg atgaaacgcc cgtcgaaggc gatggcacgc tctccccgtt cgctatcgca
     2581 gcgtggcggc taaatggcga ttggaaacta tctagcgaat gaaagcgtcc accatgagca
     2641 ccttagccgc ccccttgtca tcctgagcgt agcgaaggac cagccacccg tgtttgactc
     2701 ttacttgtgg cggaccaggc tcccctatgt catttcgaac gtagtgagaa atctcgtcct
     2761 ccaccgcgat gtctcccttt tctcctgtca acctgctgat gttcgttgca ggcaaaggtg
     2821 cggcttgggt gctgagggac gagatttctc actacgttcg aaatgacaag gtagaggctt
     2881 gggtgctact atcctacaac ggtattcttg aaatgacttc cagtaccact agccatcgga
     2941 tatgccaaca atgagcggcc caccggggcc acttaccggg cctgggcagc gtgggggcaa
     3001 ctcaatagag cgcctgcccc ctcactggcg gctgcgcgac gacaacccca acgagtggat
     3061 atggacgcct cgcgcccaac gcaggcacga cctctcgccc tggcttgagg tgtccgtgcc
     3121 catgtcggtg caggaggcgc tctggcgggc cgggcagctt ccccacccct accgcgacct
     3181 caacagccgc ctggccgagt gggtagagca ccgcgattgg gtctatggcc tcgatttccc
     3241 actcccgccc ccaccgcccg gcacccgcgt tttcatcgag ttcgagtccg tggccgatgc
     3301 ctgcatgctc ttcctcaatg gcaccctggt tggagagcac gaaggccctg gcgcgccctt
     3361 ccacttcgag ataggccccg ccctgcgcga agattcgaac cagttgctgc tcgtggtccg
     3421 cgccccgcac caggaagacc cgcaaaccgg ttggaccgag cgcacaacca gcctgcgggg
     3481 ccgcatgggc ttcggctggg acttcgcgcc acgcctggtg gccctcggca tcctgggcga
     3541 cgtgcagttg cgacataccg gaccctggaa gctgcgcgat ttatgggcgc gtcccgctct
     3601 atctgaggac ttgctccggt caacaataga cctatccgtg caagttgatg ggcctccggc
     3661 tgccggggtg cgcttcacaa tctcgcgtgg ccggcggacc gttgccgttg agaccgctac
     3721 cgtaggctcg gatgggctag cgcgcgccac catcgagcta gtccggcccg acctctggtg
     3781 gcccaacgac atgggcggcc agccccccta caacgtcagg gccgagtgcg tcgatggctc
     3841 ggatgctgtg tcctccacgt tcggcattcg caccatcgcc tgggaaggta ggccgggcgg
     3901 ctcgcctgcg gagtggcccc ttaccctggc cgtcaacggg cggcgcgtct ttcagcgcgg
     3961 ctggaactgg gtgcccgccg actcgatggg cggcccgcgt gcagcacccc tggcccgcaa
     4021 gctcctgcgc ctggcgaagg aggcccatgt aaacgtcctg cggtgctggg gcggcgccga
     4081 cccggaaacg cgggccttct acgacgagtg cgaccgcctc ggcctcctcg tgtggcagga
     4141 gttcgccctg tcgtcagccg gtatcagcaa cgtaccgccc aaagacccgg cctacctcga
     4201 ccgccttgag gcgtacgcgc gggatgtggt ggccgcccgg cgcaaccacc ccagcctggc
     4261 cttgtggggc ggcggaaacg agcttacggg agacgatggc aagccgctcg cgatggacca
     4321 cccttacccg caacgtctcg ccgccgtaat agccgcccac gacccagttc gcgccttccg
     4381 gccaagctcg cccctcggcc ccgcgtttga tgccgaccct gagcacggca acgcctacct
     4441 gtgggacgtg cacgggccct gggagtacag ccagcgttgg ccggggccgc agtactggcg
     4501 cttcaacgcc gtgcgcccta tcctccactc ggagacggga gcgcccggtg aggccagcct
     4561 cgccatgcag cgccactacc tctcgccgtc ccaccgcgcc tacaggctgc ccaccaaccc
     4621 cgcgcggcgg catcacggag gggcctggtg ggaccacagc agcacggtcg agcagctatt
     4681 cggcccaatc gaggacgaag agcttgcggt gctcgccagc caatggctcc aggccgagac
     4741 gttgcgctac tacatcgagg agacgcgccg ccggtggccc gaatcggtgg gcatctaccc
     4801 ctggcagttg aacgagccgt ggccgaacgt cgcctgcacg tcggcggtcg agtacggcgg
     4861 caggcccaag ctggcctact tcgccgtgcg gggcgcgtac aggcccctcc tgccaaccgc
     4921 ccactacagg ggtctgccct tctcccaggc cgagccgctg catgctgaga tatgggcgct
     4981 aaacgatggc cccgctcttg aagcagagtt ggaagtgtcc ttctccggcc tcgatggcgt
     5041 ggagctagcc ccaggaatca cccacgctgt gcggttggtg gcttcttcca gcacccgcct
     5101 catggacgtg gcaccctccc tgcctgggga cttcgagggt gtggtgataa tggacctggc
     5161 actgaggggc gcgcgcaaca ggtacatttt cagcagcagg caagaacacc ccttgcgcga
     5221 caccttgcgg tatcaccaat tgctgaagag catgtttgtg acacagcctg acgcaccagc
     5281 acaaccgata agaggatgac gatgagcact cacaccaggc cctccaacgt aatcacgttg
     5341 cgagagatac ttacggcagc cgagcgcggc ggctacggca taggctcctt ctcgccccgc
     5401 tataccccca tgatcctgcc tgtgctaaga gcgggccaga agatgcgttc gccactcatc
     5461 gtgcagatct ccaataacga gctaagacgc tacaccataa ccgcaggcga gttcgcggac
     5521 gagttctaca gggtgctacg cgacgagcaa atcaccgtgc ctgtggtgct gcacctcgac
     5581 cacaccaagg agttgggcgt catacgcgag gccatcgccg ccgggttcac ctccgtcatg
     5641 atggacgcct ccgagaagcc cctcgaggac aacatcgccg ccagccggga ggccgccgag
     5701 tacgcccacg ctcacggtgc ctccgccgag gccgagcttg gcatgatagg caccaccgac
     5761 aagatagaga ccgacaccga cgaggagcta tacaccgacc cactcgaggc cgaacgcttc
     5821 gtgcgcgaga cgggcgtgga cgcgctggcc gtctcggtgg gcaccgccca cggcgtctac
     5881 atggtgcgcc aacccaggat agactacgac cgcctcaagt ccatcaagtc gcttacgggc
     5941 gtgcacatgg tgctgcacgg cgggtcgggc gtgcccccgg agatgatgtg gcaggcgata
     6001 acactgccgg gcggcggggt cagcaaggtc aatatcgcca ccgaccttga ggttgccctc
     6061 ctggaggcgc tgggccgcga agagcgtatg accaacgccg agtgccgggc tttgcctccc
     6121 gacccactgg cgcgcgggcg ggcggcggtg gagcgcaccg tgtgcgacaa aatggagaat
     6181 ttcctccaca gcgcgggcaa ggcggagggg tttactttct agaccagaag gaggtcgtgg
     6241 atgattacgc gagagcaagt gcgggaggcg cgggcgcggg cgcgggacta cctggagcgg
     6301 gcgggtatag tgctcacccc cgaagaggcc gccaacatcg aggtggctga catgggcctg
     6361 gacgaactgg aacgca
//
LOCUS       DASRWZ010000334         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_919475, whole genome shotgun sequence.
ACCESSION   DASRWZ010000334 DASRWZ010000000
VERSION     DASRWZ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919475"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /gene="murJ"
                     /locus_tag="VFH60_05155"
     CDS             <1..1418
                     /gene="murJ"
                     /locus_tag="VFH60_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET6313202.1"
                     /translation="DTLFLLIIGGAVGSAFIPVFTRLVSGHEEARAWQLTSTLINASV
                     VLLSLGGIVMSFAAPALVAYLVAPGDQVDRALVVDLTRVLLLSPLLMGLGGWALGILN
                     ARQHVTLPALAPVAYNLSIIFGAVALTPLFGIYGVAWGVVLGAALHFGVQVPGLLRAG
                     MRYSFRLDLRATGVMEVAKLLGPRILGQAAFQANVVGARAIATSIAAGSVAAFNYAYL
                     LMILPHGVFAMSLATVTFPTMSALYAEGNLAGVRVTLARAVKVLLFLTLPAAAGMYAL
                     RTELVSALFQWGNFDRQSTLLVAGALGYFALGLVSYAVVEVVTRAFYALHDTRTPVAI
                     AVLTVALNLGLSALLVFGMGWDHTALALSLAVTTSVEMVLLWVFLGRKLPGWALASDG
                     LLRSIAKSGLASIAMGLALTLLMPFLWQVAGDPTQSKLSAIALTILGIGIGGATYAGT
                     ALLLRSEEIGDAARLVLRRGR"
BASE COUNT          204 a          531 c          490 g          312 t
ORIGIN      
        1 ccgacaccct cttcctgctc atcatcggcg gggcggtcgg ctcggccttc atcccggtgt
       61 tcacccggct ggtgtcgggg catgaggagg cccgggcctg gcagcttact tccacgctca
      121 tcaacgcctc ggtcgtgctg ctgagtctcg gcggcatcgt gatgagcttt gccgcgcccg
      181 ccctggtcgc ctacctcgtc gcccccggcg accaggtgga ccgcgctctg gtggtggacc
      241 tcacacgcgt cctgctcctg tcgcccctgc tcatgggcct cggtgggtgg gcgctgggca
      301 tcctcaacgc ccgccagcac gtcactctgc ccgccctcgc tcccgtggcc tacaacctct
      361 ccatcatctt cggggcggtg gcgctcaccc ccctcttcgg tatttacggc gtagcctggg
      421 gcgtggtgct cggcgcggcg ctgcacttcg gcgtgcaggt gccggggctg cttcgcgccg
      481 gcatgcgcta ttcgttccgg ctcgacctgc gggctaccgg cgtgatggag gtggcgaagc
      541 tgcttggacc ccgcatactg gggcaggcgg ccttccaggc gaacgtcgtc ggtgcccgcg
      601 ccatcgccac gagcatcgcg gcgggcagcg tggccgcctt caactacgcg tacctgctga
      661 tgatcctgcc gcacggcgtc tttgccatgt ccctggccac cgtgactttt cctactatgt
      721 ccgccttgta cgctgagggc aacctcgctg gcgtgcgcgt aaccctggca agggccgtca
      781 aggtgctgct cttcctgacc ctccccgcgg ctgccggtat gtacgccctg cgcaccgagc
      841 ttgtgtcggc cctgttccaa tggggcaact tcgacaggca atcgaccctc ctggtggcgg
      901 gagcgctcgg ctacttcgcg cttggccttg tctcttacgc cgtggtggaa gtggtcacca
      961 gggccttcta cgcgctccac gacaccagga cgcctgtcgc tatagcggtg ctcactgtgg
     1021 ccctcaacct cggactgagt gcgctgctcg tgttcggcat ggggtgggac cacacagcgc
     1081 tggcgctcag cctggccgtc accacgagcg tagagatggt gttgctatgg gtcttcctgg
     1141 gccgcaagct gcccgggtgg gcgttggcga gcgacggcct gcttcgctcc atagcaaaga
     1201 gcggcctcgc ctccatcgca atgggcctgg cgctcaccct gctgatgccc ttcctgtggc
     1261 aggtagcagg cgaccccacc cagagcaagc tctcagcaat agccctcacc atcctgggca
     1321 tcggcatcgg cggcgcgacc tatgcaggca ccgccctgct gctccgctcg gaggagattg
     1381 gcgacgcggc ccggctcgtg ttgcggcgcg gcaggtgacg agtgacgagt gacgagtgac
     1441 gagtgacgag tgacgagtgt aataagcaac aaagcggctc ttagggtaca tagtcccctt
     1501 gagccgcttt ttacgtgtta cgtgtcactc gtcactc
//
LOCUS       DASRWZ010000335         6145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1261946, whole genome shotgun sequence.
ACCESSION   DASRWZ010000335 DASRWZ010000000
VERSION     DASRWZ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261946"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..4016)
                     /gene="essC"
                     /locus_tag="VFH60_05160"
     CDS             complement(3..4016)
                     /gene="essC"
                     /locus_tag="VFH60_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HET6313203.1"
                     /translation="MPNPPVFMRAPRIWPEIPKGEVEIPTPAAAPAPASNSLATNLLP
                     RMGAVGGLGAGVFTALAGNGTVAVSLLAFSLVSTVTAIATYFSQKKNTQRMAAERQMR
                     YRALLETRKGEFAAMRDKQHAALLETDPDIAQCVERVLRLDRKLWQRSPQDADFLSVR
                     VGLGVQPFVITIKAPKQDALLEIDPLITEAHKLAEDFSQVPDVPIRLPLYQAGSAGVV
                     GPRKDVLNTTRAIISQIAAHHSPDEVKICALFPSFESEEWDWMRWLPHAWTDDRNHRL
                     LASEKDSALRLSQSLYDLLSRRRAQVAANRDSRAPAPLPYFVFVLADPRLAEFKPFLR
                     LLVNEGQALGAFAIFTADSIDALPKDIRAIVDLGAGGEGKAQLIVTAPVSSQIEFTPD
                     VVPIDLADRLARSEAPIKLKGLGGSADIPTMVPLLELLGASSVEDLGVLSRWKSSEAF
                     QSMAVPIGRRGGGEPLLLDLHERGSGPHGLVAGTTRAGKSELLQSFISSLAVNFHPYQ
                     VSFVLVDYKGGGMADAFLELPHLAGSITNLQGNLASRALLALRSELQKRQAMLAQARE
                     NHIDKYQRRYEQGELKEPLPRIIIIVDEFAELATEQPDFMKQLVSTVRVGGSLGVHLI
                     LATQKPAGVVSEEIWANTRFRICLRVARPEDSREVLRRPDAASISQPGRAYFQVGLVD
                     VFELFQSAYGGAPYVPESGQAAAPDEIMEVALDGSRNVLRLSPKPVAIQAPSGNQLQA
                     IVAHLRDVADKAAIPTLPGPWLPPLPETVTLEQVRPAEGWDGSGWRDKTTWLEPAVGL
                     LDNPARQFQGPLTLPLAREGHLIVYGAPGSGKTTMLQTLVTSVALDHSPQDVHLYLLD
                     FGGRTLSIFGGLPHVGAVILPDEGERLNRLMRYLLRELESRQSIFSQAGVTTLSAYRT
                     STRERTPAIIVVLDNYEGFSNIYTAAEDTMAQLAREGGNLGIHLIMAANSPSIKSKVS
                     GNITLAVALQMADKTDYATVLGRLGGLEPAPVTGRGLIKGTPPLEFQTALPTEAASEA
                     ERTVALRDLVRRMDEVWMGPRARPVGQRPTVVPLSDLITPSEGWPILSGDSPLSVPLF
                     LETEDLEPLPVDLGEGPHFIVTGPFQSGKTTLLQTWALALAETLSAQSLNLYLVDFRR
                     SGLNYLQPLPQVKWYIEDDQMLNAALDAITKELQERREALEQARRSATGVFDDKEFIS
                     GYPALVLVIDDFDAVRDLAQSGTKTKLEQMIRRERGLGFHVVLAGGSSDLSSAYEGWV
                     KALKELQTGVVLGSSDQNDVGVVNIRLTFNEGNKLLPPGEGFYGRRGRYRKIKAATAN
                     AGNPQLREWVARIAART"
     gene            complement(4094..4843)
                     /locus_tag="VFH60_05165"
     CDS             complement(4094..4843)
                     /locus_tag="VFH60_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET6313204.1"
                     /translation="MTYSLLATSRTPAVIIYLLDVSASMTQPLGNKRRIDVVLDALTA
                     ALRQMVFRSTKGGRLSPRYRIAMLAYSDVVYDLLDGIKTIGEVAHLGVPELSPLRTTD
                     SAKAFAQAEKLLISELPNLVDGPAPVVCHMTDGEYTGADPEPIVRRIMEMTVPDGNIL
                     VENIFISDKVLSQPVTDPTKWPGILPDTELTSDYAQKLRDLSSPIPESYRAMMQENNY
                     HLTPGALMLLPGMSPELVAMGFQMSAATPVR"
     gene            complement(4920..5654)
                     /locus_tag="VFH60_05170"
     CDS             complement(4920..5654)
                     /locus_tag="VFH60_05170"
                     /inference="COORDINATES: protein motif:HMM:NF020396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HET6313205.1"
                     /translation="MHRVIVTVKRKDEARVRDVEVPANVEMLRLADLIARAMRWESDP
                     AGQPVYYNIEAHPLGRLLEPDESLGSAGVWDGSWLVLHPVRQQVQVAPPQPEYTAPQA
                     LEATPPTWDSVASTQAESQVQQQAGAMDATTYAGEVADSPLASPFARRSIFGDTPLPV
                     PTEPAEQAPQPYTPVPDYQAQTSPSTQPASTGFSPAPAEEPELGGAFSGWRSLGIDLP
                     TGLSSAQEEPQEKRDSGGFVWRQLDD"
     gene            complement(5657..>6145)
                     /locus_tag="VFH60_05175"
     CDS             complement(5657..>6145)
                     /locus_tag="VFH60_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313206.1"
                     /translation="RVRLWGPAGERTAEFGETFRTEERWSTLNGPVGSPPHVFIAPLR
                     NSGKLQVARLMLYSDGLATLDPHELPPGDVEINKLIEEAGASPTSDDISFLDIHLRGV
                     PAQNVPARDVPVASVPDVPVPAKSGVPDKPGTLDKPDIPAKSSVSEKPKVRQVPIRRG
                     RG"
BASE COUNT         1077 a         1977 c         1952 g         1139 t
ORIGIN      
        1 gctcaggtgc gtgcggctat gcgcgctacc cactcgcgaa gttgggggtt gcctgcgttg
       61 gcggtggccg ccttgatctt gcggtagcgg ccccggcggc catagaagcc ctcgcccggc
      121 ggcagcagct tgttcccctc gttgaaggtc aggcggatgt ttaccacgcc tacgtcgttc
      181 tggtcgctgc tgccgagcac cacgcccgtc tgcaactcct tgagcgcctt cacccacccc
      241 tcataggccg agctaaggtc gctcgacccg cccgccagca cgacgtggaa gcctaggcca
      301 cgctcgcgcc ttatcatctg ctccagcttg gtcttcgtgc ccgattgggc aaggtcgcgc
      361 accgcgtcga agtcatctat caccagaact aacgctgggt atcctgatat gaactccttg
      421 tcgtcgaaca cgcccgtagc cgagcggcgg gcctgctcca atgcctcccg acgctcctgc
      481 aactccttag tgatagcgtc cagcgccgcg ttcagcatct ggtcgtcctc gatgtaccac
      541 ttcacctggg gtagaggctg caagtagtta agccccgacc gcctgaaatc caccaggtag
      601 agattgagcg attgggccga cagagtttca gcgagcgcga gcgcccacgt ctgcaagagc
      661 gtcgtcttgc cgctctggaa cggccctgtg actatgaagt gcggcccttc gcccaggtca
      721 accggcagag gttcaaggtc ctccgtctca aggaataggg gcaccgataa agggctatcg
      781 ccggacagaa tcggccagcc ctcgctcggc gtaatcaggt cggagagggg caccactgtc
      841 gggcgctggc cgacggggcg ggcgcgcggc cccatccaca cctcgtccat acggcgcacc
      901 aggtctctca gggctaccgt gcgctcggct tccgacgcgg cctcggtggg cagcgcggtc
      961 tggaactcaa gcgggggagt acccttgata aggccacgac ccgtcaccgg cgcaggctcc
     1021 aaccctccca ggcggccaag cacggtggca tagtccgttt tgtcggccat ctgtagagcc
     1081 accgccaggg ttatgttgcc gctaaccttc gacttgatgg aggggctgtt ggccgccatg
     1141 ataaggtgga tgcccaggtt gccgccttcg cgggccagtt gcgccatcgt atcttcggct
     1201 gcggtataga tgttggagaa gccttcgtag ttgtcgagca ccacaataat cgcgggcgtg
     1261 cgctcgcgcg tcgaggtacg gtacgccgag agcgtggtca cacccgcctg cgagaagatt
     1321 gactggcggc tctcaagctc gcgcagcagg tagcgcatca ggcggttcag gcgttccccc
     1381 tcgtccggca gtatcaccgc gcccacgtgg ggaagccccc cgaagatgga gagcgtgcgc
     1441 ccgccaaagt cgagcaggta aaggtgaaca tcctggggcg agtggtccag cgccaccgac
     1501 gttacgagcg tctggagcat ggtggtcttg ccggagccgg gcgcgccata aacgatcagg
     1561 tgcccttccc gcgcgagggg cagggttagc ggaccctgga actggcgggc gggattatcc
     1621 aacagcccca ccgcaggctc aagccaggtt gtcttgtcgc gccacccgga gccgtcccac
     1681 ccctccgccg ggcgcacctg ctcaagcgtc accgtctcag gaagcggcgg cagccagggg
     1741 ccggggaggg tcggtatcgc ggccttgtcg gccacgtccc gcaggtgcgc cactatcgcc
     1801 tgcagctggt tccccgatgg cgcttgtatg gctaccggct tgggagatag gcgcaagacg
     1861 ttgcggctgc cgtccagcgc gacctccata atctcgtcgg gcgcggctgc ctgccccgac
     1921 tccggcacgt agggcgcgcc gccataggcc gactggaaca actcaaacac atcgaccagg
     1981 ccgacctgga agtacgcccg gcccggctgc gagatgctgg cggcgtccgg cctgcgtagc
     2041 acctcgcggc tgtcctccgg cctcgccact ctcaggcaga tgcggaagcg ggtattcgcc
     2101 catatctcct cgctcaccac gcccgcgggc ttctgcgtcg ccaggatgag atgcacgccc
     2161 aggctgccgc ccacgcgcac cgtgctcaca agctgcttca tgaagtcggg ctgctccgtc
     2221 gcaagttcgg cgaactcgtc cacgatgatg atgatcctgg ggagcggctc tttcagttcc
     2281 ccttgctcgt agcggcgctg gtacttgtca atgtggttct ctcgcgcctg ggcgagcatg
     2341 gcctgccgtt tctgcaattc gcttcgcagg gccagcaagg cgcgcgacgc caggttcccc
     2401 tgtaagttgg tgatggagcc tgccaggtgc ggcaactcga ggaacgcgtc ggccatgccg
     2461 ccgcccttgt agtccaccag cacgaacgac acctggtatg ggtgaaaatt gacggccagc
     2521 gatgaaataa aagattggag caattcgctc ttgcccgccc gcgttgtgcc cgccaccagg
     2581 ccgtgcgggc cggagccgcg ctcgtgcagg tcgagcagca gaggctcgcc accgccgcgc
     2641 ctgccgatgg gcaccgccat agattggaac gcctcgctcg acttccaccg ggagagcacg
     2701 cccaggtctt ccacgctgct cgcgcccaac agttcgagca gtggcaccat agtggggatg
     2761 tccgccgacc cgcccagccc cttcagcttg atgggggcct ccgacctcgc cagccggtcc
     2821 gccaggtcta tgggcacaac gtcgggcgtg aactcgattt gcgacgacac gggcgcggtc
     2881 acaatgagtt gtgccttgcc ttccccgccc gcgcccaggt caactatcgc cctgatgtct
     2941 ttgggcaggg cgtctatgct gtcggcggtg aagatggcga acgcgccgag tgcctggccc
     3001 tcgttgacca gcaggcgcag gaacggcttg aactctgcca ggcgcgggtc cgccagcacg
     3061 aacacgaagt aaggcagggg tgcgggagcg cgagagtccc tgttagcggc gacctgcgcc
     3121 cggcggcggc tgagcaggtc gtacaggctc tgagagaggc gcagggcgga gtccttctct
     3181 gaagcgagca ggcgatggtt gcggtcatcg gtccaggcgt ggggcagcca gcgcatccag
     3241 tcccactcct cagactcgaa ggaggggaag agcgcgcaaa tcttcacctc gtcgggcgag
     3301 tggtgggcgg cgatctggct gatgatggcg cgagtggtgt tcaacacgtc cttgcgcggc
     3361 ccgaccacgc ccgcgctgcc cgcctggtag agcggcagac gtataggcac gtcgggcacc
     3421 tgcgagaagt cttccgccag cttgtgggcc tccgtgatga gcgggtcaat ctcaagcagg
     3481 gcgtcttgct tgggagcttt gatggttata acgaacggtt gcactcccag gcccacccgc
     3541 acggatagaa aatcggcgtc ctggggcgag cgctgccaga gcttgcggtc caggcgtagc
     3601 actcgctcca cgcactgcgc gatgtcgggg tcggtctcca acagggccgc gtgctgcttg
     3661 tcacgcatcg cggcgaactc acccttgcga gtctcaagca gggcacgata gcgcatctga
     3721 cgctcagccg ccatgcgctg ggtattcttc ttctgggaga agtaagtcgc aatggcagtg
     3781 accgtgctca cgagcgaaaa ggccagcaga gacaccgcaa cagtgccatt gcccgcgaga
     3841 gccgtgaaca cacccgcgcc caacccaccc accgcgccca tgcgtggcag caagttggta
     3901 gctagcgaat tagaggcagg cgcgggcgcg gcggcggggg taggtatctc aacctccccc
     3961 ttgggtatct ccggccatat gcgcggtgcg cgcatgaaga caggtggatt aggcacgatt
     4021 cgctctttct agtggtcagt ggtcagtggt cagtggtcag tagctttggt tctggtcact
     4081 gaccactgac cacttatctc acaggggtag cggcggacat ctggaagccc atcgccacca
     4141 actcaggact catgccgggg aggagcatca gcgcgcccgg cgtgaggtgg tagttgttct
     4201 cttgcatcat cgcgcggtag ctctcaggta tgggcgaaga gaggtcgcgg agcttctgcg
     4261 cgtagtcgct ggtcagttcg gtgtcgggca ggatgccggg ccacttcgtc gggtcggtga
     4321 cgggctgcga cagcacttta tccgagatga agatgttctc aaccaggata ttcccatccg
     4381 gcacggtcat ttccataatg cgccgcacta tcggctccgg gtccgccccc gtatactcgc
     4441 cgtcggtcat atggcacacc acaggcgcgg ggccatcaac aaggttaggt aactcgctaa
     4501 tcagcagctt ctccgcctgg gcgaaagcct tagccgagtc ggtagtgcgc aggggactca
     4561 attccggcac gcccaggtgg gccacttcgc ctatcgtctt gatgccgtcc agcaggtcgt
     4621 acaccacgtc gctgtacgcg agcatggcta tgcggtagcg cggggagagc cgcccgccct
     4681 tcgtggagcg gaagaccatc tgcctgaggg cagcggtcag ggcatccagc accacgtcta
     4741 tgcgccgctt gttccccagg ggctgcgtca tcgaggcgct cacgtccagc aggtagatga
     4801 taaccgccgg tgtcctactg gtcgcaagaa gtgaatatgt catctaaacc tccgctctaa
     4861 taatccgcac ctggctaatt gccaacactc agcactcagt actcggtact cggtactcat
     4921 tagtcgtcca actgcctcca cacgaacccg ccggaatcgc gtttctcctg cggctcttcc
     4981 tgggcgctcg atagcccggt cggcaggtct atacccaggg aacgccaacc tgaaaacgcg
     5041 cctcccagtt caggctcctc ggctggtgcc ggggagaagc ctgtgctcgc aggctgcgtg
     5101 gaaggcgaag tctgcgcttg atagtcaggt acgggcgtat aaggttgggg cgcttgctcg
     5161 gcaggctccg ttggcactgg gagcggcgta tccccgaaaa tggacctcct ggcgaacgga
     5221 gacgccaggg gagagtctgc gacctcgcct gcataagtag tggcatccat agcgcccgcc
     5281 tgctgctgga cctggctttc ggcctgggtg gacgcaacgc tatcccaggt gggaggggtc
     5341 gcttccaggg cttgaggcgc tgtgtactca ggttgcggcg gggccacctg gacttgctgc
     5401 cggacggggt gcagcaccag ccacgagccg tcccacacac ccgcgctccc caggctctcg
     5461 tcgggttcga gtagccgtcc gagggggtgg gcctctatgt tgtagtagac gggctggcct
     5521 gccgggtcgc tctcccatct catagcacgc gctatcaggt cggccaggcg cagcatctcg
     5581 acgttcgcgg gcacttccac gtcgcgaacg cgggcctcgt ccttgcgctt gactgtgacg
     5641 atcaccctat gcattactac cctctacccc gccttatggg cacctgcctg accttcggct
     5701 tttcagaaac gctagattta gcaggaatat caggcttatc aagagtgcct ggcttatctg
     5761 gaacgccaga cttagcagga accggcacat ctggaacgct agccacagga acgtcacgcg
     5821 ccggaacgtt ttgagcagga acgccacgca ggtgtatatc gaggaaactt atatcgtcgc
     5881 ttgtaggcga tgcgccagcc tcctctatga gcttgtttat ctcaacgtct ccgggcggaa
     5941 gctcgtgtgg gtcgagcgtg gcaagcccgt ccgagtagag catcagcctg gcgacttgca
     6001 gcttcccgct attcctgagc ggcgcgatga aaacgtgggg agggctgccc acaggcccgt
     6061 tgagcgtgga ccatctctcc tccgtgcgga aagtctcccc aaactcagcc gtgcgctcgc
     6121 ccgccgggcc ccacaggcgc acccg
//
LOCUS       DASRWZ010000336         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_851088, whole genome shotgun sequence.
ACCESSION   DASRWZ010000336 DASRWZ010000000
VERSION     DASRWZ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851088"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..503)
                     /locus_tag="VFH60_05180"
     CDS             complement(84..503)
                     /locus_tag="VFH60_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6313207.1"
                     /translation="MSTNGNNQDATSFMTQFDAAWNRHDLDAAVGYYADDAVVALEPA
                     PPGLPGIFTGKDEIRGFIQMLLPGFHVESSNARANGDTLTWDSRITADAFRSMGVDEA
                     ECVTEVVVRDGKVQRFTPTFSPATLAKIQQSQAAAGL"
     gene            752..2329
                     /gene="tig"
                     /locus_tag="VFH60_05185"
     CDS             752..2329
                     /gene="tig"
                     /locus_tag="VFH60_05185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET6313208.1"
                     /translation="MNITREDMPGRQVALTIELDPDTVNTALDRAYRQMVNQVNIPGF
                     RRGKAPRYMLERYVGRDELTERAVKNILPQTVQDAIVEQKIEALDVGDVEIVNLDPLQ
                     VKVIIIQPPVVELGDYSGIRVERDSVEITPEQVEEVLTELRRESAPWNEPEETRAIQE
                     GDMVYVDLEGFTTAGPLEEASRDNFPTIVGVTRAGVPEAVNQALIGMTVGEEKDIADT
                     LADDYPNEELRGKDVTYHVTVRSLKTQVLPELDEEFARKLNYDSVEALREALEANLRK
                     RATDSAESKQVNSAIEQMIAASKVEVPDMMVNEELDNMLKNLDDRLKQQRLSVRQYFT
                     YTGMTEDEWRVANREEARGHLVNNLVLREFANREGLAVEDAEIDDEIQQIIAGLDDEQ
                     RAKADEVFQNHEARHDVQDRVFQRKLMERIVGIVEGRIEAAPAPPPAEGESAEAAGGA
                     DMQATASDSGGNAGDAGDAAEADEATGTAADLEEAGGAAELLGTGDVDTRSPYETGDA
                     TGGGTPEDAPALDEGKK"
     gene            2537..>2757
                     /locus_tag="VFH60_05190"
     CDS             2537..>2757
                     /locus_tag="VFH60_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HET6313209.1"
                     /translation="MDPTQKGELIGEGRTAQVYAWGPRHVLKLYYDWWPPGNVEQEAK
                     TGKAVHAAGVPCPAVGDIVRVDGRRGLVY"
BASE COUNT          585 a          828 c          872 g          472 t
ORIGIN      
        1 gtcctttgcg tcctttgcgt tttcgtactc ttggcgttat cttggcgttc ttggcgtctt
       61 ggcggttccg tcaatagagt ttcctatagc cccgcggctg cttgcgactg ctgtatcttc
      121 gcgagggtcg cgggcgagaa ggtgggggtg aagcgctgta ccttgccgtc gcggaccaca
      181 acctcggtga cgcattccgc ttcgtctacg cccatgctcc ggaaggcatc ggcggttatc
      241 ctggagtccc aggtaagtgt gtcgccgttt gcccgggcgt tgctagactc aacgtggaag
      301 ccgggcagga gcatctggat gaagccgcgt atctcgtcct tgcccgtaaa gatgccgggc
      361 agccctggtg gggcaggctc cagcgcaacc accgcgtcat cggcgtaata gcctaccgcg
      421 gcgtcgagat cgtgccggtt ccaggccgca tcaaactgcg tcatgaacga cgttgcatct
      481 tgattattac cattcgtaga cattgaaccc ctcctaaaaa ttataggccc cactcttcaa
      541 agggtgcctc gtatcaacat agccagagta tgcccccttc cactgctcac tttccagccc
      601 gctctttatt cacttctggc ccaatcaatc caggggccta ttaatggtaa tcgggccact
      661 cagtcctcag tcctcatcac tccaacgtgc cagttcccgc ctgttctgtt ataatgatag
      721 gcaaactctc tccataaagg agttggaaga attgaatatt actcgtgaag acatgccggg
      781 cagacaggtt gccctgacaa ttgagcttga ccccgatacc gtcaataccg cccttgaccg
      841 cgcgtaccgc cagatggtca accaggtgaa catacccggt ttccgccggg gcaaggcacc
      901 tcgttacatg ctggagcgat acgtcggccg cgatgaattg accgagcgtg ccgtgaagaa
      961 catcctgccg cagaccgtgc aggacgcgat agtagagcag aaaatagaag ccctggacgt
     1021 cggtgacgtg gagatcgtga acctcgaccc cttgcaggtg aaggtgatca tcatccagcc
     1081 cccggtagtg gagcttggcg actacagcgg catacgtgtc gagcgcgact cggtggagat
     1141 tacccctgag caggtcgagg aagtgctgac cgagctgcgc cgcgagagcg ccccctggaa
     1201 cgagcctgaa gagacgcggg ccatacagga aggcgatatg gtgtacgtcg acctggaagg
     1261 cttcaccacc gcaggccccc tcgaagaagc cagccgcgac aacttcccca ccatcgtggg
     1321 cgtgactcgc gcgggcgtgc ccgaagcggt gaaccaggcg ctcatcggta tgacggtcgg
     1381 tgaggaaaag gatatagccg acacgctggc cgacgactac cccaacgagg agttgcgcgg
     1441 caaggacgtc acctaccacg tcaccgtgcg ctcgctgaag acccaggtgc tgccggagct
     1501 tgacgaggag ttcgccagga agctcaacta cgatagcgtg gaagcgttgc gggaggcgct
     1561 cgaggccaac ctgcgcaagc gcgccaccga cagcgccgag tcgaagcagg tgaatagcgc
     1621 aatcgagcag atgatcgcgg cctccaaagt cgaggtgccc gacatgatgg tgaacgagga
     1681 gcttgacaac atgctcaaga acctcgacga ccgcctgaag cagcagcgcc tcagcgtccg
     1741 ccagtacttc acctacaccg gcatgaccga ggacgagtgg cgggtagcca acagggagga
     1801 ggcccgtggt cacctggtga acaacctggt gttgcgggag ttcgccaacc gtgagggcct
     1861 ggccgtcgag gacgccgaga tagatgacga aatccagcag attatcgcgg gtctcgacga
     1921 cgagcagcgc gctaaggctg atgaggtgtt ccagaaccac gaagcccggc acgacgtgca
     1981 ggaccgcgtc ttccagcgca agctgatgga gcgcatcgtg ggcatcgtgg aaggccgcat
     2041 cgaggccgct cccgccccac ccccggccga aggtgagtcg gcagaggccg caggtggcgc
     2101 agacatgcag gccacggcct cggactccgg cggaaacgca ggagacgcag gggatgcagc
     2161 cgaagctgac gaggccaccg ggaccgccgc cgacctggaa gaggccggtg gggccgccga
     2221 attgctgggc accggggacg tagacacccg ctctccctac gagacgggcg acgccacagg
     2281 tggaggcacc cccgaagacg cccccgccct cgacgagggc aagaagtagc acgagagaag
     2341 ccggtcacag tagtctgttg tgattggcac ctgacgaaat agcggtacaa gagccggtag
     2401 tgcttgtaaa cggcagcaac gctacggacc cggagagcat ccagaccggg tagtgtgagc
     2461 aaccgcagca cggccggctt ttgattcctg gccttgtgtg ggagtggggc ctgacacggt
     2521 ccgctcaggg ggctgaatgg acccaaccca gaaaggcgaa ctcataggcg agggccgcac
     2581 cgcgcaggta tacgcctggg ggcctcgtca cgtcctcaag ctgtactacg actggtggcc
     2641 cccaggcaac gtagagcagg aggctaagac aggcaaggcg gtgcacgcgg cgggcgtccc
     2701 ctgccctgcc gttggcgaca ttgtgcgggt ggacggtagg cgcggcctcg tctacga
//
LOCUS       DASRWZ010000337         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_851187, whole genome shotgun sequence.
ACCESSION   DASRWZ010000337 DASRWZ010000000
VERSION     DASRWZ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851187"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFH60_05195"
     CDS             <1..388
                     /locus_tag="VFH60_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313210.1"
                     /translation="LRGLSDPASWVPVQAHEMGLEVGRHAGSGKVLTFAPIYAVEGGS
                     DIYDLLAVGPFVWRSGSLLEPAERRDLGLPSHDEFARLLAQEPPGAILTGFEGSLEAP
                     WIDFARERGYEMVRLPGEKVLWLLKR"
     gene            370..1263
                     /locus_tag="VFH60_05200"
     CDS             370..1263
                     /locus_tag="VFH60_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6313211.1"
                     /translation="MAAKAVGNPSRKQQGPSRAGVPLLALAVPAFALVMLLVIVGQQP
                     DGRLHLWVLDVGQGEAILLRTPRGHTAVVDGGPGATPLLNAIGRRMPFWQHDIDLVVL
                     TNPVQDRLMGLVDLLGRYDVGQVVQTEYTATGGVQAQWLRLLDASGVPVHHAARGEML
                     TFAGEPEVALEVLGPGEGGDSPMSLRLTYGPHSLLISGDARAEAEAQMVSYKGDALRS
                     QVLVVGHFGSKDVASPPFLASVRPTAAIISAGAGNRFGYPSPETLEALSAAGALVYRT
                     DLNGTVEVIADVERMWVRAER"
     gene            1270..>2100
                     /locus_tag="VFH60_05205"
     CDS             1270..>2100
                     /locus_tag="VFH60_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6313212.1"
                     /translation="MRRKRPTLQQVKTANTGIELSYRDWGGTGPPMLLLHGLGSTHRI
                     WDLAALLLSERFHVVAYDQRGHGFSEKPHVGYDLDTLSADSRGLVEALGLGPGYVVGH
                     SWGGSVALAYAGRYPRDCAGIALVDGGLVRIQDIPAIDTWEAMSELMSPTDLSPFKLA
                     DLVKKTRNRGLRALRRRFIEEFLRGTIDEQPDGALVNRLSRDNHMQILRTIWETRPEE
                     LLARVRCPALIVTAIPEAITPEQETYNTIKLLGVARAERYLTQYRSVIMPDTIHDVPL
                     H"
BASE COUNT          354 a          663 c          724 g          359 t
ORIGIN      
        1 gctcaggggg ctgtcggacc ctgcgtcgtg ggtgcccgtg caggcgcacg agatggggct
       61 ggaggtcggc cgtcacgccg ggagcggcaa ggtgctcact ttcgccccca tatacgcagt
      121 tgaaggcggc tcagatatat acgacctgct cgccgtgggg ccgttcgtat ggcggagcgg
      181 gtcgctgctg gagccggccg agcggcgaga cctggggctg ccctcgcacg acgagttcgc
      241 ccggctgctg gctcaagagc cgccgggagc catcctgacc ggcttcgagg ggagcctgga
      301 ggctccctgg atagatttcg cgcgggagcg tgggtatgag atggtgcgtc tgccaggtga
      361 gaaggtgttg tggctgctaa agcggtaggc aatccctcgc gcaagcaaca agggccgagc
      421 agggcaggcg tgccgctcct ggcgctggcc gtacctgcct tcgcgctcgt tatgctgctc
      481 gtaattgtgg ggcagcagcc cgacggcagg ttgcacctgt gggtgctgga cgttgggcag
      541 ggcgaggcga tcttgctcag gacgccgcgc gggcacacgg ccgttgtaga cggtgggcct
      601 ggggcgacgc cgctgctgaa cgcgattggc aggcgcatgc ccttctggca gcacgacatc
      661 gatctcgtgg tgctcaccaa ccccgtgcag gaccggctaa tgggcctggt agacctgctg
      721 ggccgctacg acgtgggcca ggtggtgcag accgagtaca cggccaccgg gggcgtgcag
      781 gcgcaatggc tccggctgct cgatgcgagc ggggtgccgg tccaccatgc cgctcgcggt
      841 gagatgctga ccttcgcggg cgagccggaa gttgccctgg aagtgctcgg ccccggcgag
      901 ggcggcgata gccctatgtc cttgcggctt acctacggtc cccacagcct cctcatcagc
      961 ggcgatgcgc gtgccgaagc cgaggcccaa atggtaagct acaaaggcga tgcgctgcgc
     1021 agccaggtcc tcgttgtggg ccatttcggc agcaaggacg tggcctctcc cccgttcctc
     1081 gcgagcgtcc gccccacagc cgccatcatc tcggcgggcg cgggcaatcg cttcggctac
     1141 ccctctcctg agacgctgga agctctcagt gcggccggtg ccctcgtcta ccgcaccgac
     1201 ctcaacggca cggtggaggt aatagccgat gtggaacgga tgtgggtgag ggccgaaagg
     1261 tgatgctaga tgagacgtaa acggcctacg ttgcaacagg tgaaaacggc taataccggc
     1321 atcgagctaa gctaccgcga ctggggcggc accggacctc ccatgctgtt gctgcacggc
     1381 ctcggctcta cccaccgcat ctgggacctg gccgcgctgc tgctttccga acgcttccac
     1441 gtggtggcct acgaccagcg agggcacggc tttagcgaaa agccgcatgt cggctacgac
     1501 ctcgacaccc tgtccgctga ttcgcggggc ctggtagagg cgctggggct ggggccaggg
     1561 tacgtggtgg ggcactcgtg gggcggctcg gtcgcgttgg cctacgctgg gcggtatccc
     1621 cgcgactgcg cgggcatcgc cctggtcgat ggcggtctgg tgcgcataca ggacataccc
     1681 gcgatagaca cctgggaggc catgagcgaa ctgatgtcgc caaccgacct tagtcccttc
     1741 aagctggcgg acctggtgaa gaagacccgc aatcgaggtt tacgtgccct gcgacgccgc
     1801 ttcatcgagg agttcctgcg gggcaccatt gacgagcaac cggatggtgc gcttgtgaac
     1861 cgcctgagcc gcgacaacca tatgcaaatc ctgcgcacca tctgggagac gcgccccgaa
     1921 gagttgctag ccagggtgcg gtgtcccgcc ttgattgtca cggccatccc ggaagccata
     1981 accccggagc aggagacgta caacacgatc aagctgctgg gtgtcgcccg cgccgagcgc
     2041 tacctcaccc aatacaggtc cgtaatcatg cccgacacaa tccacgacgt gcccctgcac
//
LOCUS       DASRWZ010000338         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_233782, whole genome shotgun sequence.
ACCESSION   DASRWZ010000338 DASRWZ010000000
VERSION     DASRWZ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233782"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..2110)
                     /locus_tag="VFH60_05210"
     CDS             complement(272..2110)
                     /locus_tag="VFH60_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313213.1"
                     /translation="MDAAKQATSMDRHDPRLRQLRGVLARVQARFRMLEAVRLSPIAA
                     MLGVCAAVALAVVWRFEKMLPLWGLLAVCAGLVISAVLTALAYALLRRHDLMHTALRA
                     DRALSLDERLSTALEDAVRPAPAHMRALREAQLDDALTVAHRIVPAKDLPLRLHRRQL
                     VPVAALLALFAFAILVPNPAVFTRDPEVHAQVEAERARLEEVRKAIEARPDAATNPEL
                     QELLKELAALQDELAREDLTREEAVARLSEAESKLREAIDPQAPAERAALDQLARQLE
                     SSGSDAAKKAAEALRKGDSEEAAKQLEQAGKDAAEMSPEDRKKLAEAMRQAGANVAPL
                     DPEMARRLNEAANALESDNPQAAEQAMKNLGEQVRERGQQLATQKQIEQALSQIQQSK
                     SNVARSGRQDGTPRPGGTSVAGTAGPSSGSPFSGTPVTLSGTAVALGSPVSGTPVGSG
                     TPGTPVLAQTTPGTGTPVVGSGQQGQGQQGQGQGQGQGQGQGQGQQGQPGGQGNGQPS
                     GGWGKGHNEPVYAPSQDVDAALTPVTLQGQEDPDGEQSTGSTDTDANNTGPAQVPYEQ
                     VYGSYREQAGNALNSDYIPQGYKDLVRDYFNDIDPSQAPAEEPAQP"
     gene            complement(2159..>2733)
                     /locus_tag="VFH60_05215"
     CDS             complement(2159..>2733)
                     /locus_tag="VFH60_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313214.1"
                     /translation="FWSSILKSSVASNVLTYGTMLFQLIGIPVLYLVIVTTTGNQSAP
                     GQPRMTDTPEFWYTSGIVLSSHPLIAMIISEMFISNGDPLFVYSTTQYLNGKELFVVS
                     PWLLFCIEALVFSTIFVLISIRAVQPVHIARQAKRRAPSQGLVPVEAYAGEQLPPPPP
                     PPPPYVPPPPSQAPREATVEQPREGQPPQA"
BASE COUNT          393 a          893 c          916 g          531 t
ORIGIN      
        1 gaccattacc ttattcagtt gttaatcttc ataactcgag ggctacacaa tacaaagtcc
       61 tacaagggga ctattacatc atacaaggtc tctataagta ctctgaggtg taccagtctg
      121 ccgaagggca gacttcgcaa agtgtagccc tcgatttcga tcgacgggcg tcggctggcg
      181 actgtgcttc ctagggtttg tccgtcgggc ggccaatgca tacctttgcc tgctacgagc
      241 aatcccaggg gctagtatac acccccacac ctcagggctg cgcgggttcc tccgcagggg
      301 cttgcgacgg gtcgatgtcg ttaaagtagt cgcgcacgag gtctttgtag ccctggggga
      361 tgtagtcgct gttgagggcg ttgcctgcct gctcgcggta ggagccgtag acctgctcgt
      421 agggcacctg ggccgggccg gtgttgttcg cgtccgtgtc cgtggagccg gtgctctgct
      481 caccgtcggg gtcctcttgc ccctgtagcg tcacaggggt gagggccgcg tctacgtctt
      541 gcgaaggcgc gtacacaggc tcgttgtggc ctttacccca gccgccggag ggttggccgt
      601 tgccctggcc gcccggttgg ccttgctgcc cctggccttg cccctggcct tgcccctgtc
      661 cctgcccttg accttgttgc ccctgaccct gctgtcccga cccgaccacc ggcgtgcccg
      721 tgcccggcgt ggtctgcgcg agcaccgggg taccgggcgt gcctgagccc accggggtgc
      781 ccgaaacggg cgaaccgagg gccactgcag tgccgctgag cgtcaccggc gtacctgaaa
      841 aggggctgcc gctcgacggc ccggccgtcc ccgcaaccga ggtgccgccg ggcctgggcg
      901 tgccgtcctg gcggccggag cgtgccacgt tcgacttgct ctgctggatc tgcgatagcg
      961 cctgctctat ctgcttctga gtggctagct gctgcccgcg ctcccgcacc tgctccccca
     1021 ggttcttcat cgcctgctcg gcggcctggg ggttgtcgct ctccagggcg ttggccgctt
     1081 cgttcagccg ccgcgccatc tcagggtcga gcggggccac gtttgcgccc gcctgccgca
     1141 tcgcctccgc cagcttcttg cggtcctccg gggacatctc ggcggcgtcc ttaccggcct
     1201 gctcgagctg ctttgccgcc tcctccgaat cgcccttacg caacgcctcg gccgccttct
     1261 tcgcggcgtc gctccctgag gactccagtt ggcgggccag ctggtccagg gccgcgcgtt
     1321 cggcgggggc ctgtgggtcg atggcctctc gcagcttgct ctcggcctcc gacagtcgcg
     1381 ccaccgcttc ctcccgtgtg aggtcttcgc gcgccagctc gtcttgcagg gccgcaagct
     1441 ccttgagcag ctcctgtagc tcagggttgg tagctgcgtc gggtctcgcc tctatcgcct
     1501 tgcgcacctc ctccagcctg gctcgctctg cctcgacctg ggcgtgcacc tccgggtctc
     1561 gcgtgaagac agccgggttg ggcaccagta tggcgaatgc gaacagggcg aggagcgcgg
     1621 cgaccggcac cagttgccgc ctgtgcaacc gcaagggcag gtcttttgcg ggtactattc
     1681 tatgcgctac ggtgagggcg tcatctagct gggcttcgcg caaggccctc atgtgggcgg
     1741 gggcgggcct cactgcgtct tccagcgcgg tggaaagtcg ctcgtccagc gagagggcgc
     1801 ggtcggcgcg gagggcggtg tgcatcaggt cgtgccgccg gagcagggcg tacgcaagcg
     1861 ccgtgaggac cgccgagatt accaacccgg cgcataccgc gagcaggccc cagagcggca
     1921 gcatcttctc gaagcgccac accaccgcca gcgcaacggc cgcgcacaca cccaacatag
     1981 cggcgatagg ggacaggcgc acggcctcca gcatacggaa gcgggcctgc acgcgcgcca
     2041 gcacgccccg tagctgcctc aggcgcgggt cgtgccggtc catactggtt gcttgcttgg
     2101 cggcgtccat agtgtgatta tacctttaag acgcccgcga cgccccgaag gttcccaact
     2161 aggcttgtgg cggctggccc tctcgcggct gctccaccgt cgcctccctg ggagcctgtg
     2221 agggtggcgg gggcacgtag ggaggtgggg gcgggggtgg gggtgggagt tgctccccgg
     2281 cgtaagcttc cacgggcaca agcccctggc tgggggcgcg ccttttggcc tggcgggcta
     2341 tgtgcaccgg ctgcaccgcg cgtatggaga tgagcacaaa tatggtgctg aagaccaacg
     2401 cctcgatgca gaagagcagc cagggcgaca ccacgaacaa ctctttgccg ttcaggtact
     2461 gcgtcgtgct atagacaaag agcgggtcgc cgttggagat gaacatctcg ctgattatca
     2521 tggcgataag cgggtgcgag gacaggacta tgccgctggt gtaccagaac tccggcgtat
     2581 cggtcatgcg gggctggccc ggcgcgctct ggttgcccgt cgtggtgacg atgacgaggt
     2641 agagcacggg gatgccgatg agttggaaca gcatcgtgcc gtaggtgagc acgttgctcg
     2701 ccacgctgct cttcagtatg gaggaccaga aga
//
LOCUS       DASRWZ010000339         9197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_713863, whole genome shotgun sequence.
ACCESSION   DASRWZ010000339 DASRWZ010000000
VERSION     DASRWZ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9197
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713863"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2349
                     /locus_tag="VFH60_05220"
     CDS             <1..2349
                     /locus_tag="VFH60_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6313215.1"
                     /translation="TQTMPLRSTGSSAQEQPTAVQSAEEAPPYTILPNGNIIGKAVEP
                     GVSPSLTELNAIAKARPQQQIDRSAVESEFDTIPLAGHIDAPDPVVQPNISTELMPSP
                     SRSWEGMHFYYGNGGAPPDTSGDVGPNHYVQMINSAYVVLNKSGQILAGPFAINSLWS
                     RVGGPCYNRNDGDPIVLHDQIANRWILSQFTSTAPYHECIAVSQTSDPTGLYWVYSFH
                     LSDTSFPDYPKLGVWPDGYYMGVNQFTNRAFDGTRPYVFERTRMLSGLSAQWLSTTTT
                     LGATQGLVIPSDLDGATLPPAGMPNIFAQAGGGTLNLYRFHVDWNNTANSTWTGPISL
                     PVAGFTELCSNCIPQPNTSQKLDGIGDRLMYRVTYRNYGTYEAMAVAMNVNANGYAAV
                     RWYEVRNLSTTPTIHQQGTFGNTVDHRWMASPAMDRSGNLAIGYTIGSQNRYPSVAYA
                     GRLATDPLGQLSQGEALIYAANGSQSGVSRWGDYSQLSIDPSDDCTFWYTTEYNESFD
                     WVWQTRIGSFKFPSCGGPPPAPATSTATKTNTPVPPTATATKTNTPVPPTATATKTNT
                     PVPGQPTATKTNTPLPGQPTATRTATQVPGQPSATATHTATLAPGQPTATNTPTLAPG
                     QPTSTQVPGQPTATHTVESVITPGVCDIQFPDVPPSHPFYSFVRCLACKQILGGFSDG
                     TFRPGANITRGQLSKVVANAAGFSESPGAQSYADVAPGSPFYDWVNRLSSRGAISGYA
                     CGGAGEPCGAGSLPYFRPNANATRGQVAKIVSNTFFPNCQTP"
     gene            complement(2527..3414)
                     /locus_tag="VFH60_05225"
     CDS             complement(2527..3414)
                     /locus_tag="VFH60_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6313216.1"
                     /translation="MRVIVFGATGVLGRELVPLLVRQGYSVRAPVRSPERAQSLLGAG
                     VEVCPGDLLAQETRDRLPALMAGCQAVVHIATAIPRDFGAPGAWADNTRLRTEGTRAL
                     LDAALAVGVERYIQQSIVMAYVDGGDRLLGEDTPLDASPSRAEICGPVIAMEGMVRGV
                     PTDKMRWCILRGGSFVGPGTAQDDTIRRLREGAERVPCDGSNYISPVNVSDMAEAIAA
                     ALQRAPGGSIFNIVDEPLRQGDYLDRLADLSGAPSPLRDPSRPCPPSFRCSNAAAQAV
                     LGWTPSHGIWPEPSSAGPG"
     gene            complement(3580..5223)
                     /locus_tag="VFH60_05230"
     CDS             complement(3580..5223)
                     /locus_tag="VFH60_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313217.1"
                     /translation="MWDFSSGSAWVSLIGILLLFVVLIIAASYASKRAPFSGSIVAVV
                     PQTILFAGATTIISYVLGFIPLPQVASRPASSAVLLGLLAGLVFWFVAALFHCMLRLS
                     AVDIANPSSHDMLRERLVRLNARLTNMCLSESFDDPTMVTNWMAACDEARGQRDYIQS
                     VLAARSKGGLKWVSGYGYIELWRRLHRAEEALLLVGKSEALFEFVLYDELRLRGAELT
                     NRDYLTALLRTHIGKLIPPLSLNVETVPPTSIAGAGGGGGGAGGGGGGAGGAGAAAEN
                     SSADQQARMALRAVKFTINEFRDSAWQNLVQARNRLVEAVISTALGAYGLLIFVVLQG
                     VTKDALFAASIFFLIGALVGLIDQLRINARSQAKVDDYGLEHARLLHIPLFSGLAAII
                     GVTLFAMTPKLFNAEVLTPATATPTVTVVSSAIATNQPSQTITVGVTVTARATSVTAT
                     TTATTATTATTATTATTATTTAATATPTATVAVGAGRAPDKLPPLEDIFSLTRNLFGL
                     VVAAAFGFTPGLLVAVLQKQTEQFKLNIKSTEAQEDSSK"
     gene            5529..6998
                     /gene="betC"
                     /locus_tag="VFH60_05235"
     CDS             5529..6998
                     /gene="betC"
                     /locus_tag="VFH60_05235"
                     /EC_number="3.1.6.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline-sulfatase"
                     /protein_id="HET6313218.1"
                     /translation="MPQPPNILLVMADQLMPFLTGAYGHPVVKTPHLDRLVERGVRFE
                     AAYTPYPLCAPARAAFMTGKYASDMGCYDNAAVMPADVPTIAHYLTNAGYEAILTGKM
                     HFIGPDQLHGFRRRLTTDVFPAGVGWVPTVDAEGKSGGGGHARLYVPPNVGVRPWTKF
                     LSYDEETHFRAMEYLLERGRGTPAEPFFLVASYHHPHDPFHVTQELWDLYEGEEVALP
                     VYPQDLEQTYSAMDRWVNEVHETDKVDLGHPDNLYQLRRAYYGLVTYIDRKVGELLAA
                     LERTGQRENTIVIFTSDHGDMLAERRMVQKRCFYEWSARVPLIAHFPDGSYSGRKVAE
                     PVSLIDLLPTLLDLAAVSSEQRLPVDGRSLVGLLERSDGIERPVLSEYHLEKVKAPCF
                     MVRRGRYKYIYIHGHGSQLFDLEADPGEWHNLAGQPGMQELEAELRGLILTRFDPDRI
                     EAEGAASVARRLVIKAAMERNDTHWDYQPYFDATRQYVR"
     gene            7138..7830
                     /gene="tmk"
                     /locus_tag="VFH60_05240"
     CDS             7138..7830
                     /gene="tmk"
                     /locus_tag="VFH60_05240"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET6313219.1"
                     /translation="MADSPANPGYFIVMEGIDGSGVSTQAALLARHLESIGRNAHLTK
                     EPTTGPIGSLVRQALTGRLVYAPLVHHGVEIQTDAGAYRRIDDQVMTLLYAADRLDHL
                     HQEVEPLLAGGVDVVSDRYILSTLAFQSLGASTDWIMQVNQKARRPDLTVFLDVQPEV
                     SMSRMAHRGGQVELYEDSDKSARARENYLRFIPIVRDQGEAVAVVAGGGTPQDVHALV
                     WSACVAAFPDLA"
     gene            complement(8017..8523)
                     /locus_tag="VFH60_05245"
     CDS             complement(8017..8523)
                     /locus_tag="VFH60_05245"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HET6313220.1"
                     /translation="MTEYEQPLESAGTPNPDAPGAPTPPQEVKQFDFWLGEWDCTWGE
                     GRRGSNSVRAILGGFVVQEDFNGEPAMPYHGLSVSAYSPSLKKWRQTWVDDAGTYLDF
                     VGEFADGRMVLSRTAVEESAPALQRMVWYNITPDTLDWEWQRSEDAGATWQTQWRIHY
                     RRRGETSS"
     gene            complement(8780..>9197)
                     /locus_tag="VFH60_05250"
     CDS             complement(8780..>9197)
                     /locus_tag="VFH60_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6313221.1"
                     /translation="STYYPYINRLSNRGIMGGYPCPTRPDGTPCSPDDPAIFRPTANA
                     TRGQLAKIVSNAAGYDEPVSGQIYADVPPAGQGSQFYEWIMRLTNRGVMGGYACGGPG
                     EPCDAQDRPYFRWNAQVTRGQTAKIVANTFFPACVP"
BASE COUNT         1875 a         3038 c         2635 g         1649 t
ORIGIN      
        1 acccagacca tgccgctacg gtccaccggc tcctccgccc aggagcagcc cacggccgtc
       61 cagtccgccg aagaggcccc gccctacacg atccttccca acggcaacat cattggcaaa
      121 gccgtagagc ctggcgtttc gcccagcctc acagagctta acgctatagc caaggccagg
      181 ccccagcagc agatcgaccg cagcgccgtt gagtccgagt tcgacacgat ccccctggcc
      241 ggacatatag atgccccgga cccggtggtg cagccgaaca tatctaccga gcttatgccc
      301 tcgcccagcc gctcctggga gggcatgcat ttctactacg gcaacggtgg cgcgcccccc
      361 gacacgtctg gtgacgtcgg ccccaaccac tacgtgcaga tgatcaactc ggcctacgtg
      421 gtcctcaaca agagcggcca gatactcgcc gggcctttcg ctatcaactc gctgtggagc
      481 cgagttggcg gcccttgcta caaccgcaac gacggcgacc ccatcgtgct gcacgaccag
      541 attgcgaacc gctggatact gagccagttc acaagcaccg cgccttacca cgaatgcatc
      601 gcagtctcgc agacctccga cccgaccgga ctttactggg tctactcttt ccatctcagc
      661 gatacttcct tccccgacta tcccaaactc ggcgtgtggc ccgacggcta ttacatgggc
      721 gtgaaccagt tcaccaaccg cgccttcgac ggcacccgcc cctacgtgtt cgagcgcacc
      781 cgcatgctct cgggcctgtc ggcgcagtgg ctcagcacca cgaccaccct cggcgctacc
      841 cagggcctcg taataccctc cgacctcgac ggggctaccc tgccgcccgc aggcatgcct
      901 aacatcttcg cgcaggccgg gggcggcacc ctcaacctct acaggttcca cgtggactgg
      961 aacaacaccg ccaactccac ctggaccggc cccatcagcc tccccgtggc gggcttcacc
     1021 gagctatgca gcaattgcat ccctcagccc aacacgtcgc agaagctgga cggcatcggc
     1081 gaccgcctga tgtaccgcgt cacctaccgc aactacggca cgtatgaggc gatggcggtg
     1141 gctatgaacg tcaacgccaa cggttacgcg gcggtgcgct ggtacgaggt gcgcaacctc
     1201 agcaccacac ccaccattca ccagcagggc accttcggca atacggtgga ccaccgctgg
     1261 atggccagcc ccgctatgga ccgcagcggc aacctggcta tcggctacac catcggtagc
     1321 cagaaccgct atccctcggt ggcttacgcg ggccgcctcg cgaccgaccc cctgggccag
     1381 ctttcgcagg gtgaggcgct catctacgcg gctaacggct ctcagagcgg cgtcagccgc
     1441 tggggcgatt acagccagct ttccatcgac ccgtccgacg actgcacctt ctggtacacc
     1501 accgagtaca acgagagctt cgactgggtc tggcagaccc gcatcggctc cttcaagttc
     1561 ccttcgtgcg gcgggccgcc ccccgcgcct gctacaagca cggctactaa gaccaacaca
     1621 cccgttccgc ctaccgctac ggctactaag accaataccc ccgtgccgcc tacggcaacg
     1681 gctactaaga ccaacacgcc cgtaccaggc cagcctaccg ctactaagac gaacacacca
     1741 ctgcccggcc agcctacggc tacccgcacc gctacccagg tgcccggcca gccgagcgct
     1801 accgctaccc acacggctac cctggcaccc ggccagccga ctgccaccaa cacccccacc
     1861 ctggcacccg gccagcctac ctctacccag gtacccggcc agcccaccgc cactcatacc
     1921 gtcgagtctg tcatcacgcc gggcgtctgc gacatacagt tcccggacgt gccgcccagc
     1981 caccccttct actcgttcgt aaggtgcctg gcatgcaagc agatactcgg cggcttctcg
     2041 gacggtacgt tccgccccgg cgcgaacatc acacgcgggc agctatcgaa ggtcgtagct
     2101 aacgcggcgg gcttctccga aagccccggt gcgcagagct acgccgacgt agcccccggc
     2161 agccccttct acgattgggt gaaccggcta tcgagcaggg gcgcgataag tggctacgcc
     2221 tgcggaggtg ctggtgagcc ttgcggagcg ggcagcctgc cctacttcag gcccaacgcc
     2281 aacgccacca ggggccaggt agcgaagata gtgtccaaca ccttcttccc caactgccag
     2341 actccctagt gtgagattag gagacgaaaa cgcaaagcat gccctgagcg caaccgaagg
     2401 gacgcaaagg acgcaaagaa cgcgcaaaga gtagttaggt gttctaagta aaaggaccag
     2461 ggtactaaat catgtaccct ggtccttgct ttgtgctctt gagatacctt ccagacttca
     2521 ggcgtcctac cctgggcctg cgctcgatgg ttctggccag atgccgtgcg agggtgtcca
     2581 gcccagtacc gcttgcgccg ccgcgttgct gcaccggaaa gagggcgggc acggcctgga
     2641 tgggtcgcga aggggtgagg gtgcgccgct caggtcggca agccggtcga ggtagtcgcc
     2701 ctgccgcagc ggctcgtcaa cgatgttgaa gatcgagccg ccgggcgcgc gctgtagagc
     2761 ggccgcgata gcctccgcca tgtccgacac gttgacgggg gagatgtagt tgctgccgtc
     2821 gcacggtacc cgctcggccc cctcacgcag ccgccgtatc gtgtcgtcct gagccgtgcc
     2881 tggcccgacg aatgagccgc cacggaggat gcaccatcgc atcttgtcag tgggcacccc
     2941 ccgtaccatc ccctccatcg cgataaccgg gccgcagatt tcggctctgc tgggagaggc
     3001 atcgaggggc gtatcctccc caagcaggcg gtcgccgccg tcaacgtagg ccatgacgat
     3061 gctttgctgt atgtagcgct ccacgcccac cgccagcgcg gcgtccagca gcgccctggt
     3121 gccctcggtg cgcaagcgag tattgtccgc ccacgctccc ggtgctccaa agtcgcgcgg
     3181 gatagccgtc gcgatatgga ccaccgcctg gcagcctgcc atcaaagcgg gtaagcggtc
     3241 gcgcgtctcc tgggcaagca ggtcgccggg gcacacctcc acaccggccc caagcaggga
     3301 ctgcgccctc tcaggcgagc gtacaggagc acgcactgag tagccttgtc gtaccagcaa
     3361 gggcaccaac tcacgcccca gcacgcctgt cgccccgaat acaatcactc gcatattagg
     3421 ctggaagtcc ggtagtgagt ccaagtacag cgaccacaag ccggacatct ctttgaatat
     3481 cacctgtatt caaccggcac ccgctcctta cctgactgcc tggttgccgc attggttgta
     3541 tggtttcaca tgctgctccc aacgcctgga agtccaagcc tacttgctac tgtcttcttg
     3601 tgcctcggtg ctctttatat tgagcttgaa ttgctccgtc tgcttttgta gtacggctac
     3661 aagcaggccc ggagtgaaac caaacgcggc agctacgaca aggccaaaca ggttgcgggt
     3721 caggctgaat atatcctcca gcggtggtaa tttgtcagga gcacgtcccg cccccacagc
     3781 cacagtagcg gtgggcgtcg cggtagccgc tgtggtagta gcggtagtag cggtagtagc
     3841 ggtagtagcg gtagtagcgg tagtagcggt agtagttgca gtaacagatg tagctctagc
     3901 cgtaactgtc actcctactg tgatggtctg tgagggctgg ttcgtagcaa tggcggaact
     3961 gacgacagtg accgtgggag tagccgtagc gggtgtaagc acctcagcat tgaacaactt
     4021 gggagtcata gcaaacaggg tcacaccaat tatggccgca agacccgaaa agaggggtat
     4081 gtgcaacagc cgggcatgct ccaggccata atcgtctact ttagcctgcg aacgagcatt
     4141 gatcctgagt tgatctatca ggccgaccag cgcacctatc aggaagaaga tagaggcagc
     4201 gaaaagggcg tctttagtca cgccttgtaa cacgacaaaa atcagcaacc cataggcgcc
     4261 cagagcggtg gaaatgaccg cctcaacaag gcggtttcgt gcctgcacca ggttctgcca
     4321 cgcgctatcc ctgaattcgt tgatggtaaa tttcaccgcg cgcagggcca tcctcgcttg
     4381 ctgatcggcg gaactatttt cagcagcagc accagcacca ccagcaccac caccaccgcc
     4441 accagcacca ccaccaccgc caccagcacc agcaatactg gttggaggga ccgtctcgac
     4501 gttgagggaa agtgggggaa tcaatttgcc tatgtgtgtg cgcaacaagg cagtcaaata
     4561 gtcgcggttc gtcaactccg cgccacgcag tcgcagttcg tcgtacagga cgaattcaaa
     4621 caaggcctca gacttcccaa ccagcagcaa ggcctcttca gcacggtgca ggcggcgcca
     4681 caattctatg taaccataac cggagaccca cttgagacca cccttggagc gggctgccag
     4741 tacgctctga atatagtcgc gctggccgcg ggcttcatcg caagcagcca tccagttagt
     4801 caccatcgta gggtcgtcaa agctttccga taagcacata ttcgttagac gcgcgttcaa
     4861 tctaactagc cgctcacgca gcatatcatg gcttgaaggg ttagctatat ccaccgccga
     4921 aagacgcagc atgcagtgga acaacgccgc cacgaaccag aataccaggc ccgccaacaa
     4981 acccagcaag accgcggaac tggcaggcct ggaagccact tgcggcagag ggataaagcc
     5041 tagtacatac gaaatgatcg tggtcgcgcc ggcgaacaaa attgtttggg gcaccacagc
     5101 aacgattgag ccgctaaatg gcgctctctt agaggcataa gacgcggcta tgatgagtac
     5161 gacaaagagc agcagaatac cgattagcga cacccacgca gagccggaag aaaagtccca
     5221 catgacaata ctccctatac atactgaagc ggtgtattag tgcagccggt accatcaagc
     5281 actccaacta acatgcaaca tcgtaccaca taccacaatg gccctgctgc catattagcc
     5341 gtgaagcttc tccgagcagg caattaccca aaatggccgc atgacgaaga ccccaggagt
     5401 aattgtgtcg cagggttgcc tgcccttcgg cccgctcgcg cggtgcggtc ctgataatta
     5461 cccctgtggt gatatagtgt atcattcgct acaccgcgat cagcctcacc acaccaggag
     5521 ctacgcatat gccacaacca cctaacatcc tgctcgtaat ggcggaccaa ctgatgccct
     5581 tccttaccgg cgcgtacggc cacccggtgg tgaagacacc gcacctggac cggctggtgg
     5641 agcgcggcgt ccgcttcgag gccgcctaca cgccttaccc tctctgcgcc cccgcccgcg
     5701 cggcttttat gaccggcaag tacgcctccg acatgggctg ctacgacaac gcggccgtga
     5761 tgcccgccga cgtgcccacc atcgcacact acctgaccaa cgccgggtac gaggccatac
     5821 tgacgggcaa aatgcacttc atcggcccgg accagcttca cggcttccgc cgccgcctca
     5881 ccaccgacgt gttcccggcg ggtgtcggct gggtgccaac agtagatgcg gaggggaaat
     5941 cgggtggcgg tgggcatgca cggctgtacg tcccgcccaa tgtcggcgtg cggccctgga
     6001 cgaagttcct ctcctacgat gaggagactc atttcagggc gatggagtac ctcctggagc
     6061 gggggcgggg cacgcctgcg gagccgttct tcctggtcgc ctcttaccac cacccccacg
     6121 accccttcca cgttacgcag gagctatggg acctctacga aggtgaggag gtcgccctcc
     6181 cggtctaccc ccaggacctc gagcagactt actcggctat ggaccgctgg gtcaacgagg
     6241 tgcacgagac ggacaaggta gacctcggcc accccgacaa cctctaccag ttgcgccgtg
     6301 cctattacgg gctggtcacg tacatcgacc gcaaagtagg cgagctactg gcggcgctgg
     6361 agcggacggg gcagcgcgag aacaccatcg taatcttcac cagcgaccac ggcgacatgc
     6421 tggccgagcg gcggatggtg cagaagcggt gcttctacga gtggtcggcg cgcgttccgc
     6481 tgatcgccca cttcccggat ggctcttact cagggcgaaa agtggcggag ccggtgtccc
     6541 tcatagacct gctcccgacg ctgctggacc tcgcggccgt ttcctccgag caacgcttgc
     6601 cggtggacgg caggagcctg gtcgggctgc tggagcgtag cgacggtata gagcggccgg
     6661 tcctgtccga gtaccacctg gagaaggtca aagctccctg ctttatggtc cgcaggggga
     6721 ggtacaagta catctacatc cacgggcacg gcagccagtt gttcgacctt gaagcggacc
     6781 ccggcgagtg gcacaacctg gccgggcaac ccggtatgca ggagctggag gcagagttgc
     6841 gggggctgat actgacgcgc ttcgacccgg acaggattga agcagagggc gcagccagcg
     6901 tagcaagacg gctggtgatc aaagcggcga tggagcgcaa tgatacgcac tgggactacc
     6961 aaccctactt cgacgccaca cgccagtacg tgcgttgagg ccgcacgcac agcttatgcg
     7021 tgttacagtt ccgataatgc tataatgtct ggctcacgtc agtacgtgcg ccgcgcacaa
     7081 cctggccgca cgtctcgcac acttcaatgc ctcgtttaac gggaggaccg cactgcaatg
     7141 gccgactcgc cagccaaccc tggctacttc attgtgatgg aaggtataga cggctccggg
     7201 gtgagcacgc aagccgcgct cctggcccgg cacctggaat cgatcgggcg caacgcacac
     7261 ctcaccaaag aaccgacaac cggaccgata ggctcgctcg tgcggcaggc gcttaccgga
     7321 cgtctcgtct acgcccccct ggtccatcat ggggtggaga tacagacgga cgcgggtgcc
     7381 taccgccgga ttgacgacca ggtgatgacg ctgctgtacg cggcggaccg tttggaccac
     7441 ctccaccagg aagttgagcc gctgcttgcc ggtggcgtag acgtcgtgtc cgacaggtac
     7501 atactttcga cgctcgcctt tcagtccctc ggcgcgagca ccgactggat aatgcaggtc
     7561 aaccagaagg ctcgccgccc cgacctgact gtcttcctcg acgtacagcc cgaagtctcc
     7621 atgtcgcgaa tggcccatag aggcgggcag gtggagctat acgaagacag cgacaagagc
     7681 gcgcgggcgc gagagaacta cctgcgcttc ataccgatcg tccgcgacca gggcgaggcc
     7741 gtcgcggtag tagcaggcgg cggcacaccc caggacgtac acgccctcgt ctggtccgcc
     7801 tgcgtcgctg cattccccga cctggcgtag attgtaacaa atagacggaa acgcaaagac
     7861 gcaaagaacg caaagaacgc gcagagctta ggattgttgg gttctaagcg gaaggaccag
     7921 ggtacaacat cacgaaccct ggttcttctc gcactcacca aacctacatc ctctttgcgc
     7981 gttctttgcg tctttgcgtc tttgcgtttt cgtcttctag ctgctagttt cgccccggcg
     8041 ccggtagtga atgcgccact gcgtctgcca ggtcgcgcct gcgtcttctg agcgctgcca
     8101 ctcccagtcc agggtatcgg gggtgatatt gtaccagacc atcctttgga gggccggggc
     8161 gctttcctcg accgccgtcc tgctcagcac catcctgccg tccgcgaact cccctacgaa
     8221 atcgaggtac gtcccggcat cgtccaccca cgtttgccgc cacttcttca gggaggggct
     8281 gtaagccgaa acgctcaggc cgtggtacgg catggcaggc tcgccgttga agtcctcctg
     8341 tactacgaag ccgcccagta tggcccggac gctattggaa ccgcgccggc cctcgcccca
     8401 cgtgcagtcc cattctccaa gccagaagtc gaactgtttg acctcctggg gtggtgtcgg
     8461 ggcacctggg gcgtctgggt tgggcgtgcc ggcgctttcc agcggttgct cgtactctgt
     8521 cactgtctgt ctcctttgtc gtgtttttgc ctcattccga ggtgcacgag aagaccgtgt
     8581 ccgtacatgc tggaccaccg tctggtacgc gttctgtgct cctgccctct accacaagag
     8641 cggggagcga agagttcaga atggtcaggg ttaaagcaaa ccggggccgg ggagacgcgc
     8701 cacgccacac aaaagagagg gcgggatcac tcccgccctc cgctattcta accagcctgg
     8761 accttcagca gaacgtaact taaggcacgc acgccgggaa gaaggtgtta gctactatct
     8821 tggccgtctg tcctctcgtc acctgcgcgt tccacctgaa gtacggccgg tcctgcgcgt
     8881 cgcatggctc gccaggacca ccgcacgcgt agcctcccat gacgcctctg ttggtaaggc
     8941 gcatgatcca ctcgtagaac tggctcccct gccctgctgg tggtacgtca gcgtagatct
     9001 ggccgcttac gggttcgtcg tagcctgcgg cgttggagac gatcttggct agctgccccc
     9061 tggtggcgtt ggcggtgggc cggaagatag cggggtcgtc agggctgcac ggggtgccgt
     9121 cgggtcttgt cgggcacggg tagccgccca tgatgcccct gttgctcagg cggttgatgt
     9181 aagggtagta ggtagag
//
LOCUS       DASRWZ010000340         3411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_782815, whole genome shotgun sequence.
ACCESSION   DASRWZ010000340 DASRWZ010000000
VERSION     DASRWZ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782815"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..3110
                     /locus_tag="VFH60_05255"
     CDS             306..3110
                     /locus_tag="VFH60_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313222.1"
                     /translation="MARGMPYGQEGRAGIVYQVVAAGNERRALKVFKRRFSVPSLVNL
                     SERLANFADMTGLRVCRRSVLTPRRHTELLRQYPDLTYAVLMPWIVGPTWMEVMLEKT
                     PLTPEQSLGISRAFANILAGMEERGVAHCDLSGANMILPVLASEADLQGGTFDTVELV
                     DVEQLYGPGLEKSEFVIGGSQGYASRFGQYGSWDPRADRFAGAILLVEMLAWCDERTR
                     EAAWGETYFDPSEMQTDSERYHLLKTVLAERWGAGVATLLEEVWKSETLTDCPTLGSW
                     LVAIPEQVPVKVERVEAAPDAPITMSPEEIDPEIRGMIELAQALEVEGNWNKARNTYL
                     IAQSLAPQGSKTYRYLEQMVSGLDEREREATQVLPVITAGAAAAPAGVAGAAEATDSF
                     MEETSALYTDALEAFNAGEWAAASELLTEVIRRQPDFERDGRRADQLLAEARTNQRRA
                     TDPGATQAVVMPVAPIAPVVPIVPAAPVEEAVATTVQPPANEPQVTHVVQAGEPTVPM
                     GAMPDPALAQRSQAPTQVMPVQGQAQAVPTQATQTTVVRTGRSNRSMILIPAILVAAL
                     LVFGGIAFAVLSASNDGRARAEAEQRAATSTAEAELVIIAAATEAAESTAEAAATEEA
                     NSSVSGATQTAQRSARGTITAQARATSAGLKVLGMQATETAKVAATAGAASTAQVIGM
                     ALATEQANATATAAAVAIVQGTQQVQQQATAQAQAQATQQANAQATQQAQGQIAAQTQ
                     AAINAQATVEARALATAQAHVAATARAQQPTQPPPTPKPPTPKPEPPTQPPPTQPPPT
                     QPPPTQPPPTQPPPTQPPPTQPPPTPEPPTPVPPIDNCGIAGSRNGRATPNTIPQGGS
                     LQFQITGFTPGEGFTFRWLKPNGDPYAPAAASSSFIGPDGTFTFPYTVPSDWGPGRWA
                     LQVRGASSGHEALVYFCILR"
BASE COUNT          683 a         1052 c         1132 g          544 t
ORIGIN      
        1 cacataggtt tgatgcacca gccatagtcc agacgagcga cacttacagt gcaatagtcg
       61 ggtaatccca agcgccaaag caaggaccgg ctcctggcct gggccttgca ccccagcgcg
      121 gcggatgata tactctcagg actaagcttt gctgggggag tgtttgcgtt gctccgtaca
      181 ctggggcacg tctcatttat tgcaattcgt cgcaggagaa caggagatag gtagtggcgt
      241 ttcgtcctga tgtcaaccaa gaattactta ttgatggtgt tgtttatagc atcaccgagc
      301 acccgatggc tcgcggcatg ccttacggcc aggaggggcg ggcgggcatc gtttaccagg
      361 tcgtggcggc gggcaacgag cggcgcgccc tcaaggtctt caagcgccga ttcagcgtgc
      421 cgtcactggt caacctctcg gagaggctgg cgaacttcgc cgacatgacg gggctgcggg
      481 tatgccgccg cagcgtgctc acaccccggc ggcataccga gcttttgcgc cagtaccccg
      541 acctgactta cgcggtgctg atgccctgga tcgtaggccc gacctggatg gaggtgatgc
      601 tcgaaaagac gcccctcacc ccggagcaga gcctcggcat atcgagggct ttcgccaata
      661 tcctggcggg catggaagag cgcggagtgg cccactgcga cctttccggt gcaaacatga
      721 tcctgcccgt gctggcatcc gaggcggacc tccagggcgg caccttcgat accgtcgagc
      781 tagtagacgt tgagcagtta tatgggccgg gacttgagaa gtcggagttc gtgataggcg
      841 ggtcgcaggg ctacgccagc cgcttcggcc aatacgggtc gtgggacccc agggcggacc
      901 gcttcgccgg ggccatcctg ctggtcgaga tgctggcgtg gtgcgacgag cgcacgcggg
      961 aggccgcatg gggcgagacc tacttcgacc ccagcgagat gcagaccgac agcgagcgtt
     1021 atcacctgct caagactgtg ctcgcggaga gatggggagc cggtgtggcg accctgctcg
     1081 aagaagtgtg gaagagcgag acgctgaccg attgccctac tctgggttcg tggctggtgg
     1141 cgataccgga gcaggtgccc gtcaaagttg agcgcgtaga agctgccccg gacgccccga
     1201 tcaccatgtc gccggaggag attgaccccg aaatccgggg tatgatagag ctggcgcagg
     1261 cattggaggt cgaaggcaac tggaacaaag cccgcaacac gtacctcatc gcgcaatcgc
     1321 tcgcgccgca agggagcaaa acgtacaggt accttgagca gatggtgagc ggcctcgacg
     1381 aacgcgagag ggaggccacg caggtattgc ctgtaatcac agcgggagcg gctgccgcgc
     1441 ctgctggagt cgcgggtgct gccgaggcca ccgactcttt catggaagag acgtccgccc
     1501 tctatacgga cgcgttggag gcgttcaatg caggggaatg ggcggcggct agcgagcttc
     1561 tcacggaggt aataagacgg cagcccgatt tcgagaggga tggcaggcga gccgaccagc
     1621 tacttgcgga ggctcgaact aaccagcgac gggcaaccga tccgggcgcg acacaggctg
     1681 tcgtgatgcc agtagccccg atagcgcccg ttgtgcccat tgtgcctgcg gccccggtag
     1741 aggaggctgt ggctacgacc gtgcaacctc ccgccaatga gccgcaggtg acgcatgtgg
     1801 tacaggcggg ggagcctact gtgcctatgg gggcgatgcc ggacccggcg ctggcgcaga
     1861 ggtcgcaggc tcctacgcag gtgatgcccg tgcaggggca agcgcaggcg gtgcccacgc
     1921 aggcgacaca gactacggtg gtgcgcaccg gcaggtcgaa ccgaagcatg atattgatac
     1981 cggcgatact ggtggccgcg ctgctggtgt tcggcgggat agcgttcgct gtgctctccg
     2041 cgtcgaatga cgggcgggcc agggcggagg ccgagcagcg ggccgctacg tccaccgccg
     2101 aggcggagtt ggtgattata gccgccgcta cggaagcagc tgagtctacg gcggaggcgg
     2161 ctgccactga agaagccaat tcctcagttt cgggggctac ccagacggca cagaggtcgg
     2221 cgcgcggcac cataacggcc caggcgcgtg ccacttcggc ggggttgaaa gtgctgggca
     2281 tgcaggctac agagaccgcg aaggttgccg ccacggcggg ggcagcaagc acggcccagg
     2341 tgatagggat ggcgctcgct acggagcagg ctaacgccac cgcaaccgcc gccgctgtgg
     2401 caatagtgca gggtacgcag caggtccagc agcaggcaac cgcgcaggct caggctcaag
     2461 ctacccagca ggcaaacgcg caggctaccc agcaggcgca agggcagata gccgctcaga
     2521 cccaagcagc catcaacgcc caggccactg tcgaggcgcg cgccctggcg acagcacaag
     2581 cgcatgtggc cgcgaccgcc agggcgcagc agccaacgca gccgccccct acgcccaagc
     2641 cgcctacgcc taagccggag ccgccgacgc agccaccacc tacacaaccg ccacccacgc
     2701 agccaccgcc gacgcagccg ccacctacac aaccgccacc tacacaaccg ccacctacac
     2761 aaccaccgcc gacgcctgag ccgcccacgc cggtcccacc aatagataat tgtggcatcg
     2821 caggcagcag gaacggcagg gccacgccta acaccatacc gcagggaggc tcgctacaat
     2881 tccagattac gggcttcacg ccgggtgagg gcttcacgtt ccggtggctc aagccaaacg
     2941 gcgaccctta cgcgcctgcc gcagcatcgt cctcctttat cggcccggac gggacgttca
     3001 ccttccctta tacggtgcct tcagactggg gacccggacg ctgggcttta caggtgcggg
     3061 gcgcgtcgag cgggcatgag gcgctggtct acttctgcat attgcgctga tgagggccgt
     3121 ctgcgggcgg gccggttgaa agggaatccg gtattagcct cttgacctga gcgcaggatt
     3181 aggttactat agggggtgca agcgaccgca gatgcacccc ctatggcgta gggacatttt
     3241 ctgtatatcg ttgagacgta gccatttttc gtaacacgca aggaggcatg gatgatagtc
     3301 gagtcaatgg ggcagaagaa atcgcccatg atgcgctggc tgccggcaat cgtggcgctc
     3361 gcgctcgccc tgcccgcgct cgcagtaggc accggaccca gctacgccca g
//
LOCUS       DASRWZ010000341         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_851568, whole genome shotgun sequence.
ACCESSION   DASRWZ010000341 DASRWZ010000000
VERSION     DASRWZ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851568"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFH60_05260"
     CDS             <1..840
                     /locus_tag="VFH60_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6313223.1"
                     /translation="SLCLLSNQPLLPSRKKGGADLPFHAANKNVVGGWVLTRLLLMRS
                     LRRAFGGVYVCLDPAAKRLRDEPEVPVIYCSTHPGWWDGYVSYVVNAATLRRDGYLMM
                     EEVHLAGVPFMTWAGVFGIDRHDPRKALASIEYIVGILAERPNAALYIFPQGTITHPD
                     RRPLGLYGGVGNIARKLPACAVLPVAVRYEFLLEQAPDIFVRVGAPLRFDMGRERLSS
                     AQVHARIESAMTDTADRLHADIVAGSLQSYRRVLRGRGSVNRAWERVTGAVRRVRALL
                     PRR"
     gene            856..2028
                     /locus_tag="VFH60_05265"
     CDS             856..2028
                     /locus_tag="VFH60_05265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313224.1"
                     /translation="MATPALLYHLAILALLLSLLGMVAVNLRVLPRIQGFQPPQSGAP
                     LVAILVPARDEEANIEACLASLLAQDYPHFEVWVYDDASTDRTGEIAARLASMSGGRL
                     RVVTGRSGPLPGWLGKAHACHCLYGSMREHSNPDYVLFIDADVVLRPGALGHAVGAAG
                     RLGAGLLSIFPAQVAVSMAERLAVPILLHWAVYTFLPLPIAHSPRSGPAFAAANGQFM
                     LFRREAYDAFGGHEAVRSEILEDVALARATKRAGYGAMLADGGALVRTRMYRGAREVW
                     RGYSKNAYAFFGYSPFFLAIGVLALAALYVAPVAFMLLGALLGDWLLFCLAAAQYLLG
                     VGARLLLSLRFGYRALDALLHPAGIVYMIAIELNSMRWAITGKSAWKGRSYRGLGR"
     gene            complement(2449..>3184)
                     /locus_tag="VFH60_05270"
     CDS             complement(2449..>3184)
                     /locus_tag="VFH60_05270"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HET6313225.1"
                     /translation="FGVFCFVFPFATFLMSLDLMPWGMEWMSSLLLAMLGLACGAWMV
                     VNFGWVGLWLSLGVLAAGVALEYVGVITGVPFGEYRYMGVLVPALPGGVPLAIGFAWL
                     LVVVGGLFAARWLLERANVGTRGPWPANLLGAALAVGLDLLLEPVAYHVKGYWQWQTP
                     PSQGYYGVPWSNFAAWLVAALVMNWLVSRATGGAKELRWRWLPVWLYGMNVFMFGVVN
                     VMHGFWVPGAIMALLLGALGFFGRRT"
BASE COUNT          540 a         1064 c         1006 g          574 t
ORIGIN      
        1 tctctttgcc tgctgtctaa tcagccactg ttgccttcca ggaagaaggg tggggcagat
       61 ttgccgttcc atgcggcgaa taagaacgtt gtaggcggct gggtgctgac gcggttgctg
      121 ctgatgaggt cgttgcggcg ggcgttcggt ggggtatacg tgtgcctcga cccggcggcc
      181 aagcggttgc gcgacgagcc ggaagtgccg gttatatact gctcgacgca cccaggctgg
      241 tgggatggat acgtgtccta cgtggtgaac gccgcgaccc tgcggcgtga cggctacctg
      301 atgatggagg aggtccacct ggcgggggtg ccctttatga cgtgggcggg cgtgttcggg
      361 atagaccgcc acgacccccg caaagcgctt gcctccatcg agtacatagt gggcatacta
      421 gcagagcgcc ccaacgccgc gctctacata ttcccccagg gcaccatcac ccaccccgac
      481 cgtcgcccgc tcggcctgta cgggggcgtg ggcaatatcg cacgcaaact gccggcgtgc
      541 gccgtcctcc cggtcgccgt gcgctacgag ttcctcctgg agcaggcacc cgacatattc
      601 gtgcgtgtag gcgcgccctt gcgcttcgac atgggacgcg aacgcctgtc ttcagcgcag
      661 gtccacgccc gcatcgaatc tgccatgacc gatactgccg acagactgca cgccgatatt
      721 gtggcgggca gcttgcaatc gtacaggcgg gtgctgcgcg ggcgcggctc ggtcaatcgc
      781 gcctgggaga gagtgacggg tgcggtgagg cgcgtgcggg cgctgctccc caggcgttag
      841 cggcccctct gcatcatggc cacccccgcg ctcctctacc accttgccat actcgccctg
      901 ctgctctcgc ttctcggcat ggtggcggtc aacctgcgcg tgctgcctcg catccagggc
      961 ttccagcccc ctcaatccgg cgctcccctg gttgccatac tcgtgcccgc acgcgacgag
     1021 gaagccaaca ttgaagcctg ccttgcgtcg ttgctcgccc aggactaccc ccatttcgag
     1081 gtgtgggtgt acgatgacgc ttccacggac cggaccgggg agatagccgc ccgcctcgcg
     1141 tccatgtcgg gtggcaggtt gcgcgtggta acgggccggt ctggccccct tcctggctgg
     1201 ctgggtaagg cccacgcatg ccactgcttg tacggctcca tgcgggagca ttccaacccc
     1261 gattacgtgc tgttcatcga cgctgacgtg gtgctccggc ctggcgcgct ggggcatgcg
     1321 gtgggcgcgg cggggaggct gggcgctggc ttgctctcga ttttccctgc gcaggtggcg
     1381 gtcagcatgg cggagcggct ggccgtgccc atattactgc actgggccgt ctacaccttc
     1441 ctgccgctcc ccatcgcgca ttcgccgcgt tccggccccg cctttgccgc cgccaacggt
     1501 cagttcatgc tgttccggcg ggaggcatac gacgcctttg gcgggcacga ggcggtgcgg
     1561 tccgagatat tggaggacgt ggccctggcg cgggcgacca aacgtgccgg gtacggggct
     1621 atgctggccg acgggggcgc gctcgtgcgc acgcggatgt accgtggggc gcgcgaggtg
     1681 tggcgcggct acagcaagaa cgcctacgcc ttcttcggct actccccttt cttcctggcg
     1741 attggggtgc tggccctcgc cgccctctac gtcgcgcccg tcgcttttat gctactcggt
     1801 gcgctgttgg gcgattggct gctgttctgc ctggcagccg cgcagtacct gctaggggtg
     1861 ggcgcacgcc tgctgctgtc gttgcgcttc ggctaccggg cgctagatgc cttgctccac
     1921 cccgcaggca tagtctacat gatagcgata gagttgaatt cgatgcggtg ggcgattacg
     1981 ggcaagagcg cctggaaggg gaggtcgtac cgcggcctgg gacgttagcg tactagttcg
     2041 gcacgagacg gaccgccagg acgccaagag cctacgctga gcgaagctga agggaccgcg
     2101 aaagcgccaa gaggaacaag agtggccttg tcatcctgag cgaagcgaag gatcaggtgg
     2161 gtaggacaga gtcttcaacg ttggggatga tggcggcatt aggaacattg gtgcttcaat
     2221 aggagctgtc gaacacgggt cactgatcct tcgctactct aaggatgaca gagggggata
     2281 aggtactacg cgacatgata tttgccaaac cgctatccca ctctgcgcaa cctctgcgct
     2341 ctctgcgtct ctgcggtgga ccgtacttaa cttagcgcta ccctggcgtc ctggctgttc
     2401 cgtctttttg tgtgttactg ctactgcacg cttgaagttc ccggtgtctc atgtccgcct
     2461 cccaaagaac cccagcgcgc ctagcagcaa ggccatgatc gcccccggca cccagaagcc
     2521 gtgcatgacg ttcaccaccc cgaacatgaa gacgttcatg ccgtagagcc acaccggcag
     2581 ccagcgccac cgcagctcct tcgcgccgcc cgtcgcccgg ctcaccagcc agttcatcac
     2641 gagcgccgcc accagccagg cagcaaagtt gctccagggc accccgtagt agccttgcga
     2701 cggtggggtc tgccactgcc agtagccctt gacgtggtag gccaccggct caagcagcag
     2761 gtccagcccg accgccagcg ccgcgcccag caggttggcg ggccaggggc cgcgcgttcc
     2821 aacattggcc cgctcaagga gccagcgcgc cgcgaacaac ccgccaacca cgaccagcag
     2881 ccaagcgaag ccaatcgcca ggggcacccc cccgggtagc gccggcacca gcacgcccat
     2941 gtaacggtac tctccaaagg gcacgcccgt aatcactccg acgtactcca gcgccacccc
     3001 tgccgcaagc acccccaggc tgagccacag gcccacccac ccaaagttca ccaccatcca
     3061 cgccccgcac gcaagcccca gcatcgccaa taacaggctg ctcatccact ccatgcccca
     3121 gggcatcagg tcgagcgaca tgaggaaggt ggcgaagggg aagacgaagc agaacacgcc
     3181 gaac
//
LOCUS       DASRWZ010000342         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_166348, whole genome shotgun sequence.
ACCESSION   DASRWZ010000342 DASRWZ010000000
VERSION     DASRWZ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166348"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /locus_tag="VFH60_05275"
     CDS             <1..1728
                     /locus_tag="VFH60_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313226.1"
                     /translation="AVLAGPYLTGLAIDQAIAPQDRSVTPPVSLPPRPENLPPILAAF
                     LVALVLAFVFRYAQNYIMQMIGQNVMYDLRRQIFSHLQYQSLGYYDRNPVGRLISRLT
                     NDVDALNEFIGSGLVTVAGDVVILLGVVVVMFLVNWQLALVSLAVLPLILLASFFFQK
                     LMRTTYRQQRVRIARVNAFLQENISGMLVVQLFNREKKKFDEFDKLNKDYLDANIEAL
                     RVFCYFFPVVSFLSVMAIAGLLSVGSGGVLSGAVQVGVLVMMFQYTEQAFQPIRDLAE
                     KYNIMQGAMASAERVFMLLDEEATIVDAPNTRELRKVVGDIEFRDVEFAYNPDEPVLK
                     GISFKIPAGTSVALVGATGAGKTSIISLLSRFYDVQGGQILLDGVDVREVKQQDLRRH
                     IGVVLQDPVLFSGTIARNIRLLDEDISDEQVRHAARFVNAATFIERLPDQYDHEVKER
                     GANLSVGQRQLLTFARAIAFNPSVLLVMDEATSSVDTETEALIQDALYKLMRGRTSII
                     IAHRLSTIRHVDRIIVLHKGRVVEDGTHEELMARNGYYYRLYQLQYQEQEGTAAEGVE
                     VAGEGSAVA"
     gene            complement(1734..>2535)
                     /locus_tag="VFH60_05280"
     CDS             complement(1734..>2535)
                     /locus_tag="VFH60_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313227.1"
                     /translation="VLGWLDNDRIALDAGQYLVATRDGTSFQYRNFPNLTGRESVFMN
                     QLLPDRKTMFVWVNHDFYLREVHSGDIRKVGKRSQAKDARYGIIKASPNGQYISYVGP
                     DVQNQGTRGRQLNLSFQRFSDDSRHLLVSGGVWDPSPAWSPDSSRIALVRTTAVPAGN
                     NASIFESESADTDIYLATLAGLQVRRLTSFPAAHNRDIQWTPGGNLLVASTYGSRNKA
                     FGLVWVSTTDGSAARLWTPPPGETFAQSTLFESQPSGMPPAGVDPAQP"
BASE COUNT          457 a          765 c          833 g          480 t
ORIGIN      
        1 gccgtgctcg ccgggccgta cctcacgggc ctggctatcg accaggcgat agctccacag
       61 gacaggtcgg ttacgccgcc ggtgagcctg ccgccccggc ctgagaacct gccgcccatc
      121 ctcgcggcgt tcctcgttgc gttggtcctg gcgttcgtat tccggtacgc gcaaaactat
      181 atcatgcaga tgatcgggca gaacgtgatg tacgacctgc gccgccagat attctcgcac
      241 ttgcagtacc agtcgctcgg ctactacgac cgcaacccgg taggccgcct catcagccgc
      301 ctcaccaacg acgtagacgc cctcaacgag tttataggct ccggcctggt caccgtggcg
      361 ggcgacgtgg tgatcctgct gggggtggtc gtggtgatgt tcctggtcaa ctggcagctt
      421 gccctggtga gcctcgccgt gctgcctctc atcctgctgg cgagcttctt cttccagaag
      481 ctgatgcgca ccacgtaccg ccagcagcgc gtgaggatcg cccgtgtgaa cgccttcttg
      541 caggagaaca tctccggcat gctggtggtg cagcttttca accgggagaa gaagaagttc
      601 gacgagttcg acaagctgaa caaggactac ctcgacgcca acatcgaggc gttgcgggtc
      661 ttctgctact tcttcccggt ggtgagcttc ctgtcggtca tggccatcgc ggggctgctt
      721 agcgtcgggt cgggcggcgt attgtcgggc gcggtgcagg taggtgtgct cgtgatgatg
      781 ttccagtaca ccgagcaggc gttccaaccc atccgcgacc tcgccgagaa gtacaacatc
      841 atgcaggggg ctatggcctc cgccgagcgc gtgttcatgt tgctcgatga agaggctacc
      901 atcgtggacg cgcccaacac cagggagcta cgcaaggtgg tgggcgacat cgagttccgc
      961 gacgtggagt ttgcctacaa cccggacgag ccggtgctca agggcatcag cttcaagata
     1021 ccggcgggca cgagcgtggc cctggtgggg gcgacggggg cgggcaagac gagcatcatc
     1081 tcgctcctga gccgctttta cgacgtgcag ggcggccaga ttctgctgga tggcgtggac
     1141 gtgcgcgagg tgaagcagca ggacctgcgg cggcacatag gcgtggtgct gcaagacccg
     1201 gtgctcttct ccggcacgat tgcccgcaac atccgcctgc tggacgagga catcagcgac
     1261 gaacaggtgc gccacgccgc ccggttcgtg aacgccgcca ccttcatcga gcgcctgccc
     1321 gaccagtacg accacgaggt caaggagcgc ggggccaacc tgtcggtggg gcagcggcag
     1381 ttgctcacct tcgcccgcgc tatcgccttc aacccctcgg tgctgctggt gatggacgag
     1441 gcgacatcga gcgtggacac cgagacggag gcgctgatac aagacgccct gtacaagctg
     1501 atgcggggcc gcaccagcat tatcatcgcc caccggcttt ccactatcag gcacgtggac
     1561 cgcatcatcg tgctgcacaa gggccgcgtg gtggaggacg gcacccacga ggagcttatg
     1621 gcccgtaacg gctactatta ccgcctctac cagctacagt accaggagca ggaaggcacg
     1681 gcggccgagg gcgtcgaggt tgcaggcgaa ggttcggcgg tggcttgagg cggctaaggc
     1741 tgggctgggt caacacccgc cgggggcatg ccggaaggtt gcgactcgaa gagtgtagat
     1801 tgcgcgaatg tctcgccggg aggaggggtc cagagtctcg cggctgaacc gtccgtagtt
     1861 gacacccaga ccaggccgaa cgccttattg cggctcccat aggtcgaggc taccagcagg
     1921 ttgccgccag gggtccactg gatgtcgcgg ttgtgggcgg cggggaacga ggtcagccgg
     1981 cggacctgga ggccggctaa cgttgctaag tagatgtcgg tgtcggcgct ctcgctctcg
     2041 aatatcgagg cgttgttacc ggcaggcacg gccgtcgtgc ggaccagggc gatccgcgag
     2101 ctatcgggcg accatgcggg gctggggtcc cacacgccgc ccgacaccag caagtggcgc
     2161 gagtcgtcgg agaagcgttg gaacgagaga ttcagttggc gacccctcgt gccctggttt
     2221 tggacgtccg gtccgacgta gctgatatac tggccgttgg gcgacgcttt aattattccg
     2281 taccgcgcat ctttggcctg ggaccgtttc cctaccttgc gaatatcgcc gctatggact
     2341 tcacgcaggt agaagtcatg gtttacccat acgaacatcg tcttgcggtc gggtaggagc
     2401 tggttcataa acacactttc tcgcccggtc aagttgggga agttcctgta ctggaaagag
     2461 gtgccatccc tggtggctac gaggtattga ccggcgtcca gggcgatcct atcgttatcc
     2521 aaccagccca ggacc
//
LOCUS       DASRWZ010000343         5155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_97720,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000343 DASRWZ010000000
VERSION     DASRWZ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97720"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..731)
                     /locus_tag="VFH60_05285"
     CDS             complement(243..731)
                     /locus_tag="VFH60_05285"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6313228.1"
                     /translation="MAEDRTTPEVFYDGWKEYMDTLTAALEPLTAEQLGLQAAATERT
                     VGEIAQHIVAARVLWFHDFMGEGGEEIALYSTWGKPDAPALGSSDIVRGLNATWQFMW
                     ERLSRWTPEDMRHTFPHEWRGEHYDLSRSWVVWHILEHDLIHGGEISLTLGMHGLAAP
                     RP"
     gene            complement(776..3403)
                     /locus_tag="VFH60_05290"
     CDS             complement(776..3403)
                     /locus_tag="VFH60_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET6313229.1"
                     /translation="MPAGAARKSSKPPGPTWTKIEGGKSGQPRSRASSSKSSPRPQSY
                     GGGGGGNGKPRQFQPKSKRPAFEVPSHLRREMLGLVMLVLAALCVVGLLSWLGGNVGV
                     LSVVGGVLAELFGVVAWLVPVSVGLGAVLLLVGAKAGSRWTSPLVPLGITLMLLGTMG
                     FLHLFANGQAAADAGQGGGYLGLAISTLLSDYLTRIGAGVVLVAVATLGFMLAFGVSL
                     GQLIRGVGRPTVTAGRFAGKTLGTAGSHVSRLLRERSAGRASAPGPVRINGRLAADED
                     ALAAEYEAEEETAPPVQSTHDLQTAPTQVVVLPAETTPPEKVKPEATINKLKAKSGVA
                     SDTAPQIQTASPFGYVWNLPPYNMLDTTGEVKVSQADIKAKIKVIEEALASFNVEATV
                     REVNTGPAVTQFAIEPGVGVRVSRVTSLDKDLALALAAPDIRIEAPIPGQSRIGVEVP
                     NATLQVVGLRGLMESEVYAGSKARLKVCLGKDTHGHPVVTDLAKLPHLLVAGATGSGK
                     SVFLNSMVIGFLLQFTPDDLRMLMIDPKRVELTGFNGIPHLLRPVVTDVRMEKDPQMK
                     SRGPNGTQADRERPLTAVEALKWALWEMERRYKLFAKGQRGKDNISKVFRNIEQYRTF
                     MRDNPDVKMEHLPYIVIIIDELADLMLTAPEEVETSLCRLAQLARATGIHLVVATQRP
                     SVDVVTGLIKANFPARSAFAVTSQVDSKVILDGAGAEKLLGRGDMLYTASDESKPVRI
                     QGTFVSDAESDRIVNFWRKQLPSPEDTESPPEAQGVVQPMPMPEWLGTGDPNEDSELL
                     EQAIDHVKRNRYVSVSMLQRKLRVGYNRAARLVEQMEEMGLISAANSDNKGKPREVLI
                     GAPDNRGLDVDDPLEDDEV"
     gene            3675..4070
                     /locus_tag="VFH60_05295"
     CDS             3675..4070
                     /locus_tag="VFH60_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313230.1"
                     /translation="MRRVTTALKRLPGWLQTIIVLLVFEAVARWILAPLLGVISPYAL
                     FGAIFLVVAYLLWRMVPTDKQSAIPMLVICSLSASITLIAYGETLDALEDDSSYGWVL
                     AGAACLVAGVTAAWLVMRGDKSKEATPSE"
     gene            complement(4090..>5155)
                     /locus_tag="VFH60_05300"
     CDS             complement(4090..>5155)
                     /locus_tag="VFH60_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET6313231.1"
                     /translation="LSIMSKPTSKRCFSRHLRGRRLLALLGIVFLIVILAASRTSQSR
                     RGPTTATPTSPPRLAATNTAAPAATSPPTIAPTLAPTATQAFPTPSLSNATVAVVEAR
                     PSEPAVGALPPTSTPASVPENIPAPELEPARLVSVIDGDTIEVLVGGRQARLRLIGMD
                     APETNQGPVCYGPEAAARVEELLRPVAERLLLEKDVSETDRFGRLLRYVWYEGASERS
                     MLNLELVRQGYARVATFPPDVKYQETFLQVEREARELQLGLWGNCSPTPVPPTETVPP
                     QEPLSPTVEPPTATQAPDVAPPTVATSPGYDPNGPDRDCGDFSTHAEAQAFFEAAGGP
                     ASDPHRLDGDHDGVACETLP"
BASE COUNT          911 a         1628 c         1634 g          982 t
ORIGIN      
        1 atatcacctc tgatggtatc gggggaaaga ctgactctaa cgcgatgagc aacgctatac
       61 ccggatagcc caccgtggcg atcacctcgg tagcccaggc aatcaactgc tttaagagtt
      121 cacccatggg gcaattatac ctgatacgta actgtggagc gcgataagaa acgtaaggtg
      181 aaacacaaaa gaggcccagg acataaagcc ccgtgagcct caaagcggtc tacgaatggc
      241 tcctaagggc ggggcgcggc gaggccatgc atgcccagcg tgagcgaaat ctcaccgccg
      301 tgtataaggt cgtgctccag gatgtgccag acgacccaac tgcgagacag gtcgtagtgc
      361 tccccgcgcc attcatgcgg gaaggtgtgc cgcatgtctt ccggggtcca cctcgacagg
      421 cgctcccaca tgaactgcca ggtcgcgttc agcccccgaa cgatgtcgct gctacccaga
      481 gcgggggcgt ccggctttcc ccaggtggaa tatagagcga tctcctcgcc gccttccccc
      541 atgaagtcgt ggaaccaaag aacacgcgcc gccacgatat gctgagctat ctcgcctacc
      601 gttcgctccg tggccgctgc ctggagtcct agttgttcgg cagtgagcgg ctcaagagcg
      661 gccgtcaagg tgtccatgta ctctttccag ccatcgtaaa agacttcggg cgtcgtccgg
      721 tcctcggcca tgtttccccc ttgtaccccg gctatttcag ctgccgcggc taaagctaca
      781 cttcatcatc ttccaaaggg tcgtccacgt ccaggccgcg gttgtcgggc gcgccgatga
      841 gcacttcgcg gggcttgccc ttgttgtccg agttggccgc cgagatgaga cccatctcct
      901 ccatttgctc caccagccgg gccgcgcgat tgtacccgac ccgcagcttg cgttggagca
      961 tcgacacgga tacgtagcgg ttgcgcttga cgtggtctat cgcctgctca agtagctcgc
     1021 tgtcctcgtt ggggtcgccg gtgcccagcc actcaggcat cggcatcggc tgcaccaccc
     1081 cctgggcttc cggcggcgac tccgtgtctt ccggcgaggg cagttgcttg cgccagaagt
     1141 tgactatgcg gtccgactcg gcgtccgaca cgaacgtgcc ctggatgcgc accggcttgc
     1201 tctcgtcgga ggccgtgtaa agcatgtcgc ccctacctag tagcttctcc gcgcccgcgc
     1261 catccaggat gaccttactg tccacctgcg aggtcacggc gaaggccgag cgggccggga
     1321 agttggcctt gatgaggccc gtcaccacgt ccaccgaggg gcgctgcgta gccaccacga
     1381 ggtgtattcc ggtggccctg gcaagctgcg ccaggcggca gagcgaggtc tcgacttcct
     1441 caggggcggt gagcatcagg tcggccagct cgtcaatgat gatgacgatg tagggcaggt
     1501 gctccatctt cacgtcgggg ttgtcgcgca tgaaggtgcg gtactgctct atgttgcgga
     1561 acaccttgct tatgttgtcc ttgccccgct gtcccttggc gaagagcttg tagcgccgct
     1621 ccatctccca cagcgcccac ttgagggcct ccaccgccgt gaggggccgc tcgcggtcgg
     1681 cctgggtccc gttcgggccg cgcgacttca tctgcgggtc tttctccatg cgcacgtctg
     1741 tgaccacggg gcgcagcagg tggggtatgc cgttgaagcc ggtaagctcc acgcgcttgg
     1801 ggtcgatcat gagcatgcgc aggtcgtcgg gggtaaactg caagaggaag cctatcacca
     1861 tcgagttgag aaacaccgac ttgccgcttc ccgtcgcccc ggccacgagc aggtgcggca
     1921 gcttggcgag gtcggtcacc accgggtggc cgtgggtgtc cttgcccagg cacaccttga
     1981 gccgcgcctt ggaccccgcg tatacttccg attccatcag cccgcgcagg ccgaccacct
     2041 gcaaggtcgc gttgggcacc tccactccga tgcgagactg ccctggaatg ggggcctcga
     2101 tgcgaatgtc gggcgcggcc agggcgaggg caaggtcttt gtcgagagag gtaacgcggc
     2161 tgacgcgcac gcccacaccc ggctcgatcg cgaactgggt cacggcgggg ccggtgttca
     2221 cctcgcgcac cgtggcctcc acgttgaacg aggccaacgc ctcctcgatt accttgatct
     2281 tggccttgat gtcggcttgg ctcaccttca cctcgccggt ggtgtcgagc atgttgtaag
     2341 gcggcaggtt ccacacgtag ccgaagggcg aggcagtctg tatctgcggg gcggtgtcgg
     2401 aagccacacc cgacttggcc ttcagcttat tgatcgtagc ttctggcttg accttttccg
     2461 gcggcgttgt ttcggcgggc aagactacca cctgggtagg cgcggtctgc aaatcgtggg
     2521 tagactgcac tggcggcgct gtttcctctt ccgcttcata ctcggcggct agcgcgtctt
     2581 cgtcagccgc caaccggccg ttgatgcgca ccgggccggg agcggaggct ctgccggcgg
     2641 acctctccct cagcaggcgc gaaacgtggc taccggcggt gccgagcgtc ttccccgcga
     2701 aacgtccggc tgtcacagtg ggcctgccga ccccgcggat aagttgcccc agcgacaccc
     2761 cgaaggccag catgaagccc agggttgcca cggccaccag caccacgccg gcgcctatcc
     2821 tcgtcagata gtcggacagc aacgtcgaga tggcgaggcc caggtaacca ccgccctgcc
     2881 ccgcatccgc ggctgcctgg ccgttggcga acaggtggag gaagcccatc gttcccagca
     2941 gcatgagagt gatgccgagc ggcacgagcg gcgaagtcca ccgcgaaccg gccttcgcgc
     3001 ccacgagaag caacacggcc cccagcccca cgcttaccgg caccaaccac gccacgacgc
     3061 cgaacaactc ggccagcacc ccacccacca ccgacagcac acccacgttg ccgcccagcc
     3121 agctaagtag cccgaccacg cacagagcgg cgagcaccag catcaccagg cccagcatct
     3181 cgcgccgcag gtgagacggc acctcgaacg cgggccgctt cgattttggc tggaattgcc
     3241 tcggcttgcc gttgccgcct cctccgcctc cgtagctttg cggcctgggg ctgctcttgg
     3301 aggaggaagc gcggctgcgg ggctggcctg acttgcctcc ctctatcttg gtccaggtcg
     3361 gacctggggg tttgctcgat ttcctggcgg cacctgcggg catgaaaaga cctcgtcaag
     3421 aagatgggtt ctgagagcca atttagcacg tatgtttcgg gactgccacc attatactcc
     3481 cggccacagg tgttgtggaa aactcggaac tgctcccctg tctccgtcct acgccattat
     3541 tgctcccgat gcaactgccg aattgatggg acggccaggg gtgcgcattc atatcaccgt
     3601 ccgaggcacg caaaatgaga tgcggaatgt tagcgcgtgt ccactgtggg ctgcaaactt
     3661 ccaggggagg tcaagtgagg agggtcacaa cagccctgaa gcggctgcca ggttggctgc
     3721 aaactattat agtcctgctg gtattcgaag ccgtagcgcg ttggatactt gcgccgttgc
     3781 tcggcgtgat atcgccctat gcgctcttcg gggctatttt cctggtcgta gcctacttgt
     3841 tgtggaggat ggtgcccaca gataagcaat ccgccatacc gatgctcgtc atctgctcac
     3901 tttcggcatc cattacgctg attgcgtacg gtgaaaccct ggatgccctg gaggatgaca
     3961 gttcgtacgg gtgggtcctg gcgggggctg cgtgtcttgt cgccggtgtc acagcagcct
     4021 ggttggtgat gaggggcgac aagagcaagg aagcaacgcc cagtgaatag tggctggtgg
     4081 cggcttaagt cagggcaggg tctcgcaagc aaccccgtcg tggtcgccat cgagcctgtg
     4141 agggtcgctg gccgggccgc ccgccgcctc gaagaaggcc tgggcctccg cgtgcgtact
     4201 gaagtcgcca caatcgcggt cggggccgtt agggtcgtac cctggggagg tcgcgacggt
     4261 cggtggtgcc acgtcagggg cctgcgtggc agtgggcggc tccacggtgg gggatagtgg
     4321 ctcctgggga ggcacggttt cagtgggtgg aacgggggtg ggggagcagt taccccacag
     4381 gcccagttgc agttcgcgag cctcgcgctc aacttgcaag aacgtctctt ggtacttcac
     4441 atcgggcggg aaggtcgcca cacgggcgta accttgcctc accagttcca ggttgagcat
     4501 actcctctcg ctcgcgccct cgtaccagac gtagcgtagc aggcggccaa acctgtccgt
     4561 ctcggaaacg tccttttcca gcagcagacg ctcggctacc ggcctcaata gctcctcgac
     4621 cctggccgct gcttcggggc cgtagcatac cggcccctgg ttcgtctccg gcgcatccat
     4681 gccaatcagc ctgaggcgcg cctgccggcc ccccacaagc acctctatag tgtcgccgtc
     4741 gattacgctc accaggcgag cgggttcaag ctcaggcgcg gggatattct ccggtacgct
     4801 ggcaggtgta gaagtcgggg gcagtgctcc cactgccggt tcggagggtc gcgcctctac
     4861 cactgcgacg gtcgcattgg aaagagaagg agtcggaaag gcctgggtag ccgttggagc
     4921 tagcgtaggc gcaattgtag gaggcgatgt agcagccgga gcggcggtat tagtggccgc
     4981 gagccggggc ggagaagtgg gcgtagctgt agttggaccc cggcgggact gcgaggtgcg
     5041 gctggccgcg agtatcacta tgaggaacac tatcccaagc aacgccagta agcgccgtcc
     5101 gcgcaggtga cgtgagaaac aacgcttgga agtaggcttg gacataattg ataag
//
LOCUS       DASRWZ010000344         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1263047, whole genome shotgun sequence.
ACCESSION   DASRWZ010000344 DASRWZ010000000
VERSION     DASRWZ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263047"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFH60_05305"
     CDS             <1..939
                     /locus_tag="VFH60_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HET6313232.1"
                     /translation="IGLNLANVAGGMFMANWPLALITLLAAVLIAVYTRGFMSMLPIL
                     LGVVFGYLVSLLNTLLGNPFPALGLIDTSAIDKAAWLGIPNFVTPTVDWNAALLIAPV
                     AIVLVAENTGHVKAISGNMGRNLTPKLGHAFLGDAAGTALSAVGGGTGQTTYAENIGV
                     MAMTRVYSIAVFVVASATAILLGFIPKFAALIQSIPVAVMGGISLLLFGLIAATGGRI
                     WVDGAVDFAKQRNLIVGGVTIVVGAINIAPPATPVAFNIGGFELSGIALATLVAIVLN
                     QLLAFRERRDEATAADTERDSTPAPGVADAQPEPSR"
     gene            complement(1135..2679)
                     /locus_tag="VFH60_05310"
     CDS             complement(1135..2679)
                     /locus_tag="VFH60_05310"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET6313233.1"
                     /translation="MMNLQQLLTGAAIQGAHLLAPSHGDVEITHIAENSRLVEPGSLF
                     VAVRGSVHDGHKFVPDALKRGAVAVAGEREERPEGLPDEVPYVHVPDDRLAVAQLSAA
                     LFDYPARKLKVVGVTGTDGKTTTSTLIHAILKAAGRKADVITTIRAEIGGREYDTGFH
                     VTTPEAFDVQRYLHLMVEAGTEIVVLETTSHALDQGRVACVDYDVAVVTNVTHEHLDW
                     HGSWENYMASKARLFEALNGSYRKPGTPKVAVINADDRSYTWLKKIPSDMQWVYGLER
                     NAQPDISADEIEYTPGGTRFVALTPVADIPVRLRLPGKHNIYNAMAAIGAALALGADK
                     EAVRKGLASVEQIRGRMEWVREAAPFGFDMVVDFAHTPNALEKTLELARQIVQPRDGR
                     VIVVFGSAGLRDREKRGLMGHVAGRLADVTVITAEDPRTEQVEAISADIARAMREEGR
                     AEGVDFFQVHDRAEAIAFAVQRARPGDIVLTCGKAHESSMCYGTDETPWDEFAAVRAA
                     LSRKST"
     gene            complement(2716..3177)
                     /locus_tag="VFH60_05315"
     CDS             complement(2716..3177)
                     /locus_tag="VFH60_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313234.1"
                     /translation="MYIRASDGTSFQLEIVGYQYPELIDVEYDSNWLWMSMSATLPKG
                     SWSIIDPFLLTYEVAKLADWLDAIATHSETQDEMGFVEPNISFEVVRRVSGQSCLRVY
                     FRAECRPPWAGANETGGTDIIAEFALADVDLRLAAESLRTQLSIYPQRTAL"
     gene            3328..3672
                     /locus_tag="VFH60_05320"
     CDS             3328..3672
                     /locus_tag="VFH60_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017652356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET6313235.1"
                     /translation="MQLEDYFEFRAADDIRIKGLRVGIETVLYNYLHRKWTPEEIARN
                     HPTLSLEQVYATILYYLHNRQEVEQYLDNWLEWNKRLREEQVRSPHPGIIKLRAHLKE
                     REREQDRHRTAG"
BASE COUNT          701 a         1219 c         1140 g          736 t
ORIGIN      
        1 atcgggctga acctggcgaa cgtggcgggc ggcatgttca tggcgaactg gccgctggcc
       61 ctcataacgc tgctggcggc ggtgctcata gcagtgtaca cacgcggctt catgtcgatg
      121 ctgcccatcc tgctgggcgt ggtgttcggc tacctggtgt ccctgctcaa caccctcctg
      181 ggcaacccct tccccgccct gggcctcata gacaccagcg ccatagacaa ggcggcctgg
      241 ctcggcatcc ccaacttcgt cacccccacg gtggactgga acgcggcgct gctcatcgcc
      301 ccggtagcta tcgtgctggt ggccgagaac accgggcacg tcaaggccat ctccggcaac
      361 atgggccgca acctcacccc caagctgggc cacgccttcc tgggcgacgc ggcgggcacg
      421 gcccttagcg cggtgggcgg cggcaccggg cagaccacct acgctgagaa catcggcgtg
      481 atggcgatga cccgcgtcta ctcgatagcg gtgttcgtgg tcgcctccgc caccgcgatc
      541 ctgctcggct tcatacccaa gttcgcggcc ctcatccagt cgatcccggt ggcggtgatg
      601 ggcggcatct ccctgttgct gttcgggctg atcgcggcga cgggcggacg catctgggtg
      661 gacggcgcgg tggacttcgc caagcagcgc aacctgatcg taggcggcgt gaccatcgtg
      721 gtgggcgcca tcaatatcgc cccacccgcc acccccgtcg ccttcaacat cggcggcttc
      781 gagctttccg gcatcgccct ggcaacgctg gtggctatcg tgctgaacca actcctcgcc
      841 ttccgcgagc gccgcgacga ggcgacagcg gcagacacag agcgcgacag caccccggcc
      901 cccggcgtag ccgacgccca acccgaaccc tcccggtagc aggaggggca gagacgaaaa
      961 cgcaaaggac gcaaagaacg cgcagagatt aaggattgat gttctaagca ggaggaccag
     1021 ggtacacgat gatgtactct ggtccttctt atgccaacta aaccctaaca ctctttgcgc
     1081 gttctttgcg tcctttgcgt ctttgcgttt tcgtctcccg ttgctgtttt ggaatcaggt
     1141 ggattttcgg gataaggcag cgcgtacggc ggcgaattcg tcccaggggg tctcgtcggt
     1201 gccgtagcac atggaacttt cgtgggcctt gccgcaggtg agcactatgt cgccggggcg
     1261 ggcgcgttgg acggcgaagg ctattgcttc ggcgcggtcg tgcacctgga agaagtctac
     1321 cccctcggcg cgcccttcct cccgcatggc acgggctata tccgccgaaa tcgcctccac
     1381 ctgctcggtg cgcgggtcct cggcggtaat gaccgtcacg tcggccagcc gccccgccac
     1441 gtggcccata agcccgcgct tctccctatc gcgcagcccc gccgagccga acaccactat
     1501 cacgcggcca tcgcgcggct gcactatctg gcgggcaagc tccagcgtct tttccagcgc
     1561 attgggcgtg tgagcgaagt ccaccaccat atcgaagccg aacggggcgg cctcccgcac
     1621 ccactccatc ctgccgcgta tctgctccac cgaggcaagc cccttccgca ccgcctcctt
     1681 gtccgcgccc agcgccaggg ccgcgcctat tgcagccata gcgttgtaga tgttgtgctt
     1741 gccgggcagg cgcagcctca ccgggatgtc ggccacgggc gtgagagcca cgaagcgcgt
     1801 gccgcccggg gtgtactcga tctcgtccgc gctgatgtcg ggttgggcgt tgcgctccag
     1861 gccgtacacc cactgcatgt cggagggtat ctttttcagc caggtgtacg aacggtcgtc
     1921 cgcgttgatg actgcgacct tgggcgtgcc gggcttgcgg tagctgccgt tcaacgcctc
     1981 gaacagccgg gctttggatg ccatgtagtt ctcccagcta ccgtgccagt cgaggtgctc
     2041 gtgcgtgacg ttcgtgacca ccgcaacgtc gtagtccacg caggccaccc gcccctggtc
     2101 cagcgcgtga gaggtggttt ccagcacgac tatctcggtg cccgcctcga ccatgaggtg
     2161 caggtagcgc tgcacgtcga acgcttcggg ggtggtcacg tggaagccgg tatcgtactc
     2221 ccggccgcct atctcggcgc gtatggtggt gataacgtcg gccttgcgcc cggcggcctt
     2281 gaggatggcg tggatgaggg tggaggtggt cgtcttgccg tcggtgccgg ttacgcccac
     2341 caccttgagc ttgcgagccg ggtagtcgaa cagcgccgcc gaaagctggg ccaccgccaa
     2401 cctgtcgtcg ggtacgtgaa cgtagggcac ctcgtccggc aggccctccg gccgctcctc
     2461 ccgctccccc gcgacggcta ccgcgccccg cttgagggcg tcgggtacga acttgtggcc
     2521 gtcatgcacg ctaccgcgca cggctacgaa gaggctgccc ggctccacga ggcgggagtt
     2581 ctcggctatg tgggtaatct cgacatcacc atgcgagggc gcgagtagat gcgcaccttg
     2641 tatggccgcg ccggtgagta gttgctgtag gttcatcatg gtctctctta agtacgtctt
     2701 accggccaac ggacctcaca aggcagtccg ttgtgggtag atgctcaact gtgtcctgag
     2761 cgactccgcg gccagacgta gatcaacgtc ggccaacgca aactcagcga ttatatcagt
     2821 tccaccggtc tcgttcgcgc cggcccaggg tggtctgcat tcagctctga agtacacgcg
     2881 caagcaactc tgtccgctca cccgccgtac tacctcgaac gagatgttgg gttcgacaaa
     2941 gcccatctcg tcctgtgttt cggaatgggt cgctatagcg tcgagccagt cagccaattt
     3001 ggctacttcg taggtaagca ggaatgggtc tatgatcgac cacgaaccct ttggcagggt
     3061 agcagacata ctcatccaga gccagttcga gtcatattcg acatctatta gctccggata
     3121 ctggtagccc acgatctcta gctgaaacga cgtaccatca cttgcacgaa tgtacacgct
     3181 acgtccgctc ctcttccgtc ccgcacaatg gttgcactat gctcctgatt aggcaatgac
     3241 aacatgaatg tggaatccgt ctcttgcatg gtgttgcttt agtataatcc gaaaaacgcc
     3301 aagtgctcca gatttgcgag gtagcatatg caactagaag actattttga gttccgcgca
     3361 gccgatgaca tccgcatcaa gggcctacgg gtaggtatcg agacggtcct gtacaactat
     3421 ctgcaccgca agtggacccc ggaggagatc gcgcgcaacc accccacact gtcgttggag
     3481 caggtgtacg ccaccatcct ctactacctg cacaaccgcc aggaggtaga acagtacctc
     3541 gacaactggt tggagtggaa caagcggctg cgcgaggagc aggtgcgctc gccccacccc
     3601 ggcatcatca agctgcgcgc ccacctcaaa gagcgcgagc gagagcagga ccggcaccgg
     3661 accgctgggt aggctgggta attacgaatt acgaattacg aattacggaa agagacgaaa
     3721 acgcaaagac gaaaagtacg caaagaacgc gcaaagttta ggattgtatg gtgctaggta
     3781 cgagtaattc gtaatt
//
LOCUS       DASRWZ010000345         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1263071, whole genome shotgun sequence.
ACCESSION   DASRWZ010000345 DASRWZ010000000
VERSION     DASRWZ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263071"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..320)
                     /locus_tag="VFH60_05325"
     CDS             complement(108..320)
                     /locus_tag="VFH60_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6313236.1"
                     /translation="MQKVPFGSLTPRELEVLKLLAGGKTNKEIAWQMRITVNTAKKHV
                     SQVLAKLDVSSRMEAALLYIRSGTLR"
     gene            849..>3088
                     /locus_tag="VFH60_05330"
     CDS             849..>3088
                     /locus_tag="VFH60_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313237.1"
                     /translation="MLAAFAAPFATPTGAAAARAGTFSDPAFERVWSRTDKPIEERKT
                     ARSWMWGPEGFYSAYEPYLEGPGGQHLVTYFDKSRMEINAPAGDRNSQWYVTNGLLVV
                     DMMSGRIQTGNNSFAPFAPNNAPVAGDAKTSGNAPTYATLAKVASLKGDNRAPNRTGQ
                     AIQEGLGRNGNVGILQNLSGFSKYGVYEPTLGHNIPDVFWQYMNQRGPVFKDGRYADD
                     TVVDWLFAMGYPITEPYWIQINVGGQDRWVLMQAFQRRILTYSPYNPEGWKVEMGNVG
                     RAYFDWRYSGQTPPPAQTPPPAQPTPTTVPAATPRMGINPTQGDLNTAITVSGAGFQA
                     NVNVTLVVENSGAAYQRNLGVVGTNNKGEFSTQVRVPGDASKLGEVKITAISGAQRVS
                     QTYKLVFDPSIKVTPDRSVVSGGNIKVQGAGYPARSDGRVGILVNDADMVWLQNVRSN
                     DQGNFEATINIGRRPAGTQLQVIATLGGNYKAVSGRLSVYDAPVPLSISVNPSVLKVG
                     QTALVSGTGYQPNVRVSIGLGYTEVVEWVASAVADGNGNINASFTLGGRWANAGQITL
                     FGAAPNQVATAKINVAGSGGGPVVPQGLDMRVNTYTAGGQTLFKIRGFGWQAGQAVNL
                     TLFSVDGAVNSLVGSGTVKDDGSFQVNFPAVAPWAGRADLGLRATAPNGAVLSVRNVP
                     VVTITGSTVPGTSHAVVARALPANTPVEVVVRIDGQGDKVIGSGVTDVTGAITLHVSV
                     PRIPSQ"
BASE COUNT          610 a         1022 c          914 g          542 t
ORIGIN      
        1 gctccaggga taatcttccc taagagccgc tttgttgctt gttgcacctc tgtgcctaac
       61 gctttgcgct ttaccgtaat tcgtaattcc taattcgtaa ttcgtaatta cctgagcgtg
      121 ccggagcgga tgtagaggag ggcggcttcc atgcggctgc tcacgtccag cttggcgagc
      181 acctggctta cgtgcttctt ggcggtgttt accgtgatgc gcatctgcca ggctatctcc
      241 ttgttcgtct tgccccccgc aagcagcttg agcacctcta actcgcgggg cgtgaggctt
      301 ccaaacggaa ccttttgcac atccgcatcg tctttattgt aggggccgtg gtttgcgggc
      361 atgtccatgc gcagctctac acctttggtc gcagcgcctc agctaccgta tgttgcctga
      421 tgtagccggg cacacacagt atatagaact tatgttccaa aatcaagtgg tatttgcacg
      481 tggaggtacg caaaatggcc tttgtgagcc agtctggaag gtctaaaatc ccgtcccaag
      541 aaattggggt ctattcagcc cctacctctt gtcgtataat cgagtaccac ctaagacgta
      601 ggtggctttc gcccatgtag ctgattagtt ccacaagccc cggatgcata atagcatcag
      661 ggagcttggt ctacagagcc atctagcgca tctgaacgcc gcactcctcc aacctataca
      721 aaatagatgc atacatacca aaatcaaaaa gcacagaacg gagcgataca atgaacgtat
      781 tatcaaatca aactcagagc cgcaggtccg ggcttaggaa tctggcgacc gcgacagtag
      841 gcctgtcgat gctggcggcc ttcgcggccc ccttcgcgac ccccacgggc gcggcagctg
      901 cacgggcagg cactttctcg gacccggcgt tcgagcgcgt gtggagccgc accgacaagc
      961 ctatcgagga gcgcaagacc gcgcgctcat ggatgtgggg gccggaaggc ttctactccg
     1021 cctacgagcc ttacctcgaa ggccccggcg gccagcacct cgttacctac ttcgacaaga
     1081 gccgcatgga aatcaacgcc cccgccggcg accgcaactc ccagtggtac gtcaccaacg
     1141 gcctccttgt ggtggacatg atgtccggcc gcatacagac gggcaacaac tcgttcgccc
     1201 ccttcgcgcc caacaacgcc cccgtggccg gcgacgccaa gacgagcggc aacgccccta
     1261 cctacgccac cctggctaag gtcgcttccc tcaagggcga caaccgcgca cccaaccgca
     1321 cagggcaggc catccaggag ggcctcggtc gcaatggcaa cgtgggcatc ctgcagaacc
     1381 tctccggctt ctccaagtac ggcgtgtacg agccgacgct gggacacaac atccccgacg
     1441 ttttctggca gtacatgaac cagcgcggcc ccgtcttcaa ggacggccgg tacgctgacg
     1501 acaccgtggt ggactggctc ttcgcgatgg gctaccctat caccgagcct tactggatcc
     1561 agatcaacgt cggcgggcag gaccgctggg tgctgatgca ggctttccag cgccgcatcc
     1621 tgacctactc gccctacaac ccggaaggtt ggaaggtgga gatgggcaac gtgggccgcg
     1681 cctacttcga ctggcgctac tccggccaga cccctccccc tgcccagacc ccgcctcccg
     1741 cgcagccgac ccctaccacc gtacccgctg ctacccccag gatgggcatc aaccccaccc
     1801 agggcgacct gaacacggcc atcacggtct caggcgcggg cttccaggct aacgtcaacg
     1861 tcacgctcgt cgtggagaac agcggcgcgg cctaccagcg caacctgggc gtggtcggca
     1921 ccaacaacaa gggcgagttc tcgacccagg tgcgggtgcc cggcgatgct tccaagctgg
     1981 gtgaggtaaa gatcaccgcc atctccggcg cgcagcgcgt gtcgcagact tacaagctgg
     2041 tgttcgaccc ctctatcaag gtcacgcccg accgctcggt cgtcagcggc ggcaacataa
     2101 aggtgcaggg cgcgggctac cccgccaggt cggacggccg tgtcggtatc ctcgtgaacg
     2161 acgctgacat ggtgtggctc cagaacgtgc gcagcaacga ccagggcaac ttcgaggcga
     2221 ccatcaacat cggccgccgc cccgcgggca cgcagctaca ggtgatcgcc acgttgggcg
     2281 gcaactacaa ggcggtatcg ggcaggctga gcgtgtacga cgcgcccgtg cccctgtcga
     2341 tcagcgtcaa ccccagcgtg ctcaaggtgg gccagacggc cctggtgagc ggcaccggct
     2401 accagcccaa cgtcagggtc tcgatcggcc tcggttacac cgaagtggtc gagtgggtgg
     2461 cctccgcggt ggctgacggc aacggcaaca tcaacgccag cttcacgctg ggcggaaggt
     2521 gggccaacgc cgggcagata acgctcttcg gggccgcgcc caaccaggtc gccaccgcca
     2581 aaattaacgt ggcagggagc ggtggcgggc cggtagtgcc ccagggcctc gatatgcggg
     2641 tgaacaccta cacagcaggc ggccagacgc tcttcaagat acgcggcttc ggctggcagg
     2701 caggccaggc ggtgaacctg accctcttct cggtggacgg cgcggtaaac agcctggtgg
     2761 gcagcggcac ggtgaaggac gacggctcct tccaggtcaa cttcccagcc gtggccccgt
     2821 gggcaggcag ggcggacctg ggcctgcggg ctaccgctcc caacggcgcg gtgctctcgg
     2881 tcagaaacgt acctgtggtc accatcaccg gctcgacggt ccccggcacg agccatgccg
     2941 tggtcgcccg cgccctaccg gctaacaccc ccgtagaggt ggtggtgcgc atcgacggcc
     3001 agggtgacaa ggtgataggt tccggcgtga cggacgttac gggtgcaatc accctccacg
     3061 tatccgtccc caggatccca agccagaa
//
LOCUS       DASRWZ010000346         3634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1057340, whole genome shotgun sequence.
ACCESSION   DASRWZ010000346 DASRWZ010000000
VERSION     DASRWZ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057340"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..1204
                     /locus_tag="VFH60_05335"
     CDS             359..1204
                     /locus_tag="VFH60_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6313238.1"
                     /translation="MRERTQVKTIEVNGTGLHYVERGEGPPVVFVHGGLGDFRTWLPQ
                     VETFSAGYRAISYSRRVHYPNAWPPDYTLSSMPLHVEDLAAFIAVLGLGRTHIVGNSY
                     GSYISLHLALKYPHLVRALALAEPPVHPLLRSLPGGEELFEGFMRDAWGPAGEAFGRG
                     DLEGGTRLFIEGAVGPGQWDKLTPRVREAMLKDAPELAVATHTPFETHMPPLACDDLA
                     RIEAPVLLLRGEHSPHMYTLINNELARCLPRAEQALVPQASHVLHAHNQEAHDRLVLG
                     FFEGC"
     gene            complement(1226..2671)
                     /locus_tag="VFH60_05340"
     CDS             complement(1226..2671)
                     /locus_tag="VFH60_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313239.1"
                     /translation="MEFWDFLQSWGVYAPVLLYVWIAAAVVVAYRRTRQAYWLTPLPF
                     GVLGVTYALLFPRVFEPDEVLDLPYVVALAFPLGIVIQYVLSSVVSSWGGTYHRPVSP
                     HMLDVLLFRRPRPTGKLPEPPPGLRHETVLEPRKDLPLVVTMACAVFVCFLVSYSSRG
                     LGFCQWLDVALERSGCIRQLSFGTYVEEVAYSPDGRLLAAGGIGHVVKIWSTRDWHLV
                     RTLSEPTDWVENLAFSPDGKTLATWSEDDKLRMWGVEDGRLIRVLEPGAGEDVYSFSS
                     LLYSPDGTTIAFTGEPSPDTDAVWLWNAQDGTLIRRISAFAHQIAFSPDGKLLAHVEG
                     HREIALHRISDGVVVRRLPLPTGQLLDLYYSPDGSQLAVLSDRRVHFIMSTDGTVVRT
                     LDLGEEYTRDSALSSDWKYVAAPTEKFNVAGRYNKTVSLGLWQLSPDRLVSRWPVGDS
                     TVSGIAYAPDNTTLATGLGYDLIRIWRLPRP"
     gene            complement(2691..>3634)
                     /locus_tag="VFH60_05345"
     CDS             complement(2691..>3634)
                     /locus_tag="VFH60_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6313240.1"
                     /translation="DRGVGGVFLMDYSGSPISNITSPAQLKTLTAGLRDLASGWPLPL
                     LIAVDQEGGQVARLKTRYGFPATVSQQYLGSRNDLAATRGQAQTTARTLASHGINLNF
                     APVVDVNANPYNPVIGGKARSFSSSPAVVANHSLEVIKAHREQGVFCTLKHFPGHGSS
                     TADSHLGFVDVTDTWSEKELQPYQQIIGAGECDVVMTAHIFNSRLDPNHPATLSFKVV
                     NNLLREKLGFWGVVLSDDMQMKAISSHYNLQQALGLSISAGVDMITISPARAYQTDLA
                     SRAIDIIKGFVISGAISETRIEESYTRIKRLKERLGR"
BASE COUNT          673 a         1149 c         1140 g          672 t
ORIGIN      
        1 atcagcttga gcgagtacct ccactcgata gacgtgctgg agcaggtact gcgcaccctc
       61 gccacctagc ccctttaacc cctcccagaa tgcacggtgg cagttcagaa gagacgaaaa
      121 cgcaaagacg caaaggacgc aaagaacgcg caaagagaat aaaggttaga gatgttgaaa
      181 aggaccaggg tacctgatgg cgtactctgg tccttttgct cagaacatgt tcttaatctt
      241 ggcgtggttc ttggcgtccc ttcggctacg ctcagggcag gcgtggcggt ttcgtctcct
      301 tacaaggtgg cacatgctat acaatgggct gggataacca agcaccggga ggcagggatt
      361 gcgagagcgc acgcaggtca agacgataga ggtgaacggg accgggctgc actacgtcga
      421 gcggggggag gggccgccgg tggtgttcgt gcacggcggg ctgggcgact ttcgcacctg
      481 gctgccccag gtcgagacat tttccgccgg ttaccgcgcc atctcctaca gccgccgcgt
      541 ccactaccct aacgcctggc cgcccgatta caccctctcc tccatgccgc tacacgtgga
      601 ggacctggcg gccttcatcg cagtcctggg gctgggccgc acgcatatcg tgggcaactc
      661 ttacggctcg tacatctccc tgcacctcgc gctcaagtac ccccacctgg tgcgcgccct
      721 cgcgctggcc gagccgcccg tccacccgct gttgcgctcg ctgcccggcg gggaggaact
      781 gttcgagggc ttcatgcgtg acgcctgggg tccggcggga gaggcgttcg ggcggggcga
      841 cctcgagggc ggcacgcggc tgttcataga gggcgcggtc ggtcccggac agtgggacaa
      901 gctcacaccc cgcgtgcgcg aggccatgct caaggacgcc cccgaactgg ccgtcgccac
      961 ccacaccccc ttcgagacgc acatgccccc gctcgcctgc gacgacctcg cccgcatcga
     1021 ggcccccgtg ctgctgctac gcggcgagca cagcccacac atgtacacgc tcatcaacaa
     1081 cgagctagcg cgctgcctgc cccgtgccga gcaggcgctg gtgccgcagg cctcgcacgt
     1141 gctgcacgcg cataatcagg aggcgcacga taggttggtg ctggggttct ttgaggggtg
     1201 ctgaccgccg cgacatttgg cggggtcagg gtctgggtaa gcgccagatg cgtatcaggt
     1261 cgtagcccag tccagtggca agggtggtat tgtcgggcgc gtaagcgatg ccactgactg
     1321 tggagtctcc tacgggccac ctgctcacca acctgtcggg ggacagttgc cagaggccca
     1381 ggctcaccgt cttattgtac ctccccgcaa cattgaattt ctccgtcggg gccgcaacgt
     1441 acttccaatc gcttgaaaga gcgctatcgc gtgtgtactc ctcgcccagg tcgagggtcc
     1501 ttaccacggt accgtctgtg gacatgatga aatgcacccg ccggtctgag agcacggcca
     1561 attggctccc gtcgggggag tagtagaggt ccaatagttg gccggttggg agcggcaacc
     1621 ttctgactac tacaccgtct gagatcctgt gcagggctat ctccctatgt ccttccacgt
     1681 gagctaatag ctttccgtcg ggcgagaaag caatctggtg ggcgaacgcg gagatcctgc
     1741 gtataagggt gccatcctgc gcgttccaca gccagaccgc gtccgtatcg gggcttggct
     1801 ctccggtgaa ggctatagtc gtgccatcgg gcgaatagag caagcttgaa aagctataga
     1861 catcttcccc ggctcccggc tccagcaccc tgatgagtct gccatcctcc actccccaca
     1921 tgcgtagctt atcgtcctcc gaccatgtgg cgagcgtctt gccgtcgggc gagaaggcga
     1981 ggttttctac ccagtcggta ggctccgaga gggtgcggac caggtgccaa tcgcgcgtgc
     2041 tccatatctt caccacgtgc ccgatgcccc cggcagccag cagcctcccg tccggggagt
     2101 acgcaacctc ttctacatag gtaccaaagg agagttgccg tatgcagccg cttcgttcta
     2161 aagccacgtc cagccactga cagaagccga ggccccggct gctgtagctc accaggaaac
     2221 agacgaagac ggcgcaggcc atcgtcacca ccaggggcag gtcctttctg ggttccaaaa
     2281 cggtctcgtg ccgcaggccg ggtggaggct caggtagctt acccgttggc ctgggcctgc
     2341 ggaatagcag tacatccagc atgtgcgggc ttacgggcct gtgataagtc ccgccccagg
     2401 aacttacaac tgacgaaagc acgtactgga taactatgcc cagggggaaa gcaagggcaa
     2461 ccacatacgg caagtccagg acctcatccg gctcgaagac cctcgggaac agtagcgcat
     2521 atgtcacacc gagtaccccg aaaggcagcg gtgtcagcca gtaggcttgc cgggtccgtc
     2581 tatacgcgac aacgaccgcg gccgcgatcc agacgtacag caaaacaggg gcatacacgc
     2641 cccagctttg caagaagtcc cagaactcca tcgcgccctc gtttgcaggg ctaccgcccc
     2701 agccgctcct tcaaccgctt tatccgcgtg tacgactcct cgatccgcgt ctccgaaatg
     2761 gccccgctta ttacgaagcc tttgatgatg tcgatagcgc gggaggcgag gtcggtctgg
     2821 taggcgcggg cggggcttat ggtgatcatg tccaccccgg cgctgatgct caggccgagc
     2881 gcctgttgca ggttgtagtg gctggagatc gccttcatct gcatgtcgtc cgacagcacc
     2941 acaccccaga agcccagctt ctcgcgcagc aggttgttca ccaccttgaa cgacagcgtg
     3001 gccgggtggt tggggtcgag ccgcgagttg aagatgtggg ccgtcatcac cacgtcgcac
     3061 tcgcccgcgc cgatgatctg ctggtaaggc tgtagctcct tctcagacca ggtgtccgtc
     3121 acgtccacga aacccaggtg cgagtcggcg gtagagctgc cgtgccccgg aaagtgcttg
     3181 agcgtgcaga ataccccctg ctcccggtgg gcctttatca cttccagcga gtgattcgcc
     3241 accacagcgg ggctggagga gaagctacga gccttgcccc cgatgaccgg gttgtagggg
     3301 ttggcgttca cgtccaccac gggggcgaag ttgaggttga tgccgtgtga tgctagcgtg
     3361 cgggcggtgg tctgggcctg gccgcgggtg gccgccaggt cgttgcgcga gccgaggtac
     3421 tgctggctca ccgtggctgg gaagccgtag cgggtcttga gccgcgccac ctgcccgcct
     3481 tcctggtcca ccgcgatgag gaggggcagg ggccagccgc tcgccaggtc acgcaggccc
     3541 gcagtgagcg tctttaactg ggcgggggag gtgatgttgc ttatgggcga gccgctgtag
     3601 tccatcagga agacgccgcc cacgccgcgg tcgc
//
LOCUS       DASRWZ010000347        10352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_234692, whole genome shotgun sequence.
ACCESSION   DASRWZ010000347 DASRWZ010000000
VERSION     DASRWZ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10352
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234692"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VFH60_05350"
     CDS             <1..1299
                     /locus_tag="VFH60_05350"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET6313241.1"
                     /translation="VLFFLQILGSALGAVPVYWLARDRLGVKLAAAGAAMAYLLYPTL
                     LHTALNPFQVRLFALTLLLFGFYFYERGNWKLFLACALMAVLARTDVALVVMMFALYG
                     LLTRRKWQYVVPPLLVGLGYFALSTFVITPSFAYPGALTPPPPGTQLDMSCWPCGMSP
                     QLAYYAHLGRSGPEILQFVLLHPLDAFSRAVLEPGLLGAKLFYIVSLLLPLGFLSVLA
                     PKPLVLGLPIVALNLLSLRGSQIDLESHYSLMIIPGLVAAAIYGADNLRKRWVRRGDG
                     GDLEARAVRRLQLGGLALAVWALVLTLPYGNPVVRVIRFHESADYVRAANELAAMIPG
                     DAKVAVTSKLAPRVLRRRYIYNFPPAPYSPYNIGSHTHPGFGPPYVELDYILADPNNE
                     SFESPDRFVNGQNAIAFIQSSPEWKLESSKEGILLFKRQP"
     gene            1357..1719
                     /locus_tag="VFH60_05355"
     CDS             1357..1719
                     /locus_tag="VFH60_05355"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET6313242.1"
                     /translation="MSNAGEYNPIEPVGVELAESENRLYINWSDGRTTQYAVDWLRWQ
                     CPCALCRGEMGAPGRLDFVKELSPQETTMQGAEQIGRYAIMFFWADGHHDGIYTYDWL
                     RRNSQDEQSAPPGTQAPG"
     gene            1739..2653
                     /locus_tag="VFH60_05360"
     CDS             1739..2653
                     /locus_tag="VFH60_05360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6313243.1"
                     /translation="MAIEIPTREQASRTIPPASSPELEARLVEAIEPWLQHMKWRVDF
                     DGWRERRIWQEEHQEANLRDVRSVLGSRVLGVRLLDLGAGMGGLSVALLREFGGAGLA
                     VQATDYNPSYAQIAQLRAKRYGLDLPITVAAGEALPYADGLFDVVLCLDVLEHVQDVE
                     AVLGETYRVLKPGGVVLTTVPNRRAFRDPHYHLPLINWLPTPLADRIIERVGRSKSGG
                     PLQDRQEHSELNTYTWGKFARVASGVGFRVRDQVRHRILSGEIRQLHGWRRALLAGVR
                     RLGLLDPLYRAYRYGWQGTYQVMLVKPR"
     gene            2650..3795
                     /locus_tag="VFH60_05365"
     CDS             2650..3795
                     /locus_tag="VFH60_05365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313244.1"
                     /translation="MKLALYNLTTTTRWGGVETFVWEVAAQMASRGHKVTIFGGRGNV
                     ERRRIAGVRVRVLPYIDRDAWRKLPLLSRQYGLTKLLERLSMAPFALPALLAGRFDIV
                     HIQKPYDLPVGALARLSGAKLLFGCHGKDFWPGDRLFVRFANASVSASRFNAEQVRAR
                     YGIDPVVVYNGVDLTRFTPEGPNDPQVLAQYGLAPSSHDSPTILYAGRLVRWKGVEYL
                     IKALTEVVPENTVLWVAGEGEYRPVLEQMAREVGVDDRVRFLGRLGQDDLAALYRNCA
                     MLVATSFVNETFGMALCEAMACGTPVVASDFGGFREVVVEGETGFRVSPQDPRDLAAK
                     ITALLASPQLRDRMGRAGRRRVLEMFSWKAVGDRLEEVYRCLVPSST"
     gene            3771..4973
                     /locus_tag="VFH60_05370"
     CDS             3771..4973
                     /locus_tag="VFH60_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313245.1"
                     /translation="MSRTLLYVAYPMRLDLGAANAIQTYSTVRELQKVLPGVRLVVPR
                     WLRESSAFEELGALHLPRPAANKLSKFLPWAGWSYLERTVYSFMLLALLVAWRLTGRG
                     YDALYVRDAVCAAWLALLRPLHGSRVIYEVHDLEASHPSKASKWPRAFWRRFLPWLDR
                     AALTRSDALVSLTGAFKEWVVRRRLRQPGHVAVIPDAYDPDVYFPAHTDTARSELGLP
                     VAVPIIGYTGLTFAYRGLDLLVRAFAQVRRQHPEALLVLVGGRQHEIEELKQLVRECR
                     LPAESVRFPGQVPQPVSAQYLNASDVLVIPETVTVMTASPLKLFEYMAVGKPIVCKDM
                     PALREILDGDSAVFFEAGNIEALAEGIARVLDDPQAGMRLGLSALERSAGYTYRARAE
                     KIAEVVRS"
     gene            4982..6424
                     /locus_tag="VFH60_05375"
     CDS             4982..6424
                     /locus_tag="VFH60_05375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6313246.1"
                     /translation="MRTVPRAESREVTGHWWSDRALVALLVLFGLVAPLVTHRIYASD
                     EIQYFAYTHSLFFDRDFDFGNEYMHFYASNPQKFEAIYTDLYLKKEDLTGLPINVAPI
                     GTGLFWLPSYAVAHLFVTAANGLGANIPADGYSQPYITAICLTSYLLGCAGLLLCYLI
                     SRRLFGKRVSAVGVAVTWLATPLVFYTVVAPPWSHATSLFTVTLFLWYWLRTRRESGR
                     GWREWLVLGLCAGLMMLVREQDALFLIVPGLEVAVRLVARWRKSKLADRGALMRPIGG
                     LMLMGVTAAIVFIPQLVAYRAITGRFGPSKVVSGKFTWTAPNAHNVLFSPEHGLVPWT
                     PVVVVALLGLVLLWRRDRLLAGALAVAFFVQVYIAGSFLTWQSAGSFGQRRFINSTAI
                     FVLGLVAVLSWVLARGVPRWALGALALLFVAWNGGLLIQYALWTSDQRQGLEWATVLQ
                     GQLEMPAKAARLLWTYITDREQFYSETGPR"
     gene            6511..7620
                     /locus_tag="VFH60_05380"
     CDS             6511..7620
                     /locus_tag="VFH60_05380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6313247.1"
                     /translation="MISDRLLRSVCCVNPECIQQWGTGTLLARQERAALQCPRCSTVY
                     AMNPAGYIELMPPQRGQHTSLYVSQEAEFDAALDYKRIGIPLLGAAVRQRAIREMLKP
                     ASSDRLIELGCGNGKFVFWNRKAVEWAVGVDPAPLFAREALQGLDLCLSDARALPFED
                     GAFTAAISIDVLEHLPLEDIRAFLLEANRVLDEGGRLFLYSNTREGSRLDFIVRSVRR
                     LSRWLGERGMLDDRRDRLRKSDHVKALATYPDLEKVLAEHGFGVERVVFWNGLFQSII
                     ENLVVKLGESALRRAKGRGGAVLDEAQGSAGGEDSMRRDVKQRLARRGPEYRAMMLLT
                     RLMWLDLALFGGWRAGPYFVLARRVRGAPGVGVAD"
     gene            7623..8804
                     /locus_tag="VFH60_05385"
     CDS             7623..8804
                     /locus_tag="VFH60_05385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313248.1"
                     /translation="MLYVAGAVEVFGTSGGSIHIQEVACGLRALGHEVLVLSLRGKAG
                     DGSGTLECGVPVRATRWRKELALLGLPKVRRAMRAFRPDVVMERFYNFAGAGVLASHR
                     QGIPSLLEVNAPMTDPPGSLKTRLDRLLLGSMRRWAVAQGRWSDAIVTPLASTVPPEV
                     GREKIHELPWGANVERFDPARRLQGEERQALAAELGLALEHPVAAFLGSFRRWHGVEH
                     FAEAARRLIDGGAPISFLAVGGGPELEGLRARVASWGLPPGRFVFTGPQPHERVPELL
                     SLADIGVAPFDVASHAPLAEYGFYWSPLKVFEYMAMALPVVTIDTPPLNDIVREGQEG
                     LLFPTGDIDGLISALARLAGDAGLRERLGQSGRQRVVANYSWQAHCAALDRLLRKIAH
                     A"
     gene            8797..10134
                     /locus_tag="VFH60_05390"
     CDS             8797..10134
                     /locus_tag="VFH60_05390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313249.1"
                     /translation="MPERAQPGSLGVCLLTDVFPPHSGGSGWSTYYLGKALSERGHRV
                     TVLRPVYDSGKARPARRTSRYEALDVEEVLVPRPPGWAAKAGIGRAWSQRLAGRLMEK
                     RASALVEKGAVDVLHGQHQVSGVAASSAAFMSCRPVVSVVTVRDYWPLCPSSTRLFED
                     AAGDPFECRDCHRLRAYLSCVRSRGVRDTAKLPVSYTRWLNTLAASRQLAKSDAVIAV
                     SSYVGRELDRSSRVPASRLRVIPNLVDLPSVGRALEGAWPLNDISHEDRFLLFAGKLD
                     TNKAAWQLPRLLARAGVSLPVVIAGDGPWRDRIAREASELGLDFHFYDWLDNDAVLRL
                     MNKAVALLFPSAWQEPLSRVLLEGCATGAAIVALDTGGTADVVEHGTSGWLAHDLEEF
                     AAGVRAVTSDAGLNARLRRGARERAERKFAAPVVCAQVEGLYRSLLERKGPRQ"
     gene            10131..>10352
                     /locus_tag="VFH60_05395"
     CDS             10131..>10352
                     /locus_tag="VFH60_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313250.1"
                     /translation="MRVANCVRAIFPQHGYGGLERAATALTRNLLLRGVDVDLFTRAL
                     PPGKPLRVPPGAKGRVSVHPVRYGRLPLPP"
BASE COUNT         1609 a         3206 c         3669 g         1868 t
ORIGIN      
        1 gtgctcttct tcctgcaaat actgggatcc gcgttgggcg cggtgcccgt gtactggctg
       61 gcgcgcgacc gcctgggtgt gaagctggcg gcggcaggcg cggcgatggc gtacctgctc
      121 taccccacgc tcctgcacac cgccctcaac ccgttccagg tgcggctttt cgcgctgacc
      181 ctcctgctgt tcggcttcta cttctacgag cgaggcaact ggaagctatt cctggcttgc
      241 gcgcttatgg cggtgctggc ccgcaccgac gtggcgctgg tggtgatgat gttcgcgctt
      301 tacgggctgc tgacgcggcg caagtggcag tacgtcgtcc ctcccttgct cgttgggctg
      361 ggctacttcg cgctgtccac cttcgtcatt acgccctcgt tcgcgtaccc tggcgcgctg
      421 accccgcccc ctccaggcac ccaactcgac atgtcgtgct ggccgtgcgg gatgagtccg
      481 cagcttgcct actacgccca cctgggccgg agcgggcctg agatcttgca attcgtgttg
      541 ttgcacccgc tggacgcttt cagcagggcg gtgctagagc cggggctgct gggagccaag
      601 ctgttttaca tagtgagcct gctcctgccg ctggggttcc tctcggtcct tgcccccaag
      661 ccgctggttc tcggcctgcc catcgtcgcg ctgaacctgc tgtcgctgcg ggggtcgcaa
      721 atcgacctgg agtcccacta cagcctgatg atcataccgg ggctggtggc ggcggctatc
      781 tacggggcgg acaacctgcg taagcggtgg gtgcggcgtg gggacggtgg ggacctggaa
      841 gccagggcgg tgcgaaggct gcaactgggt gggctggccc tggcggtgtg ggcgctggtt
      901 ttgacgctgc cctatggcaa cccggtggtg cgcgtcatca ggttccacga gtcggcggat
      961 tacgtgcggg cggctaacga gcttgcggcg atgattcctg gggatgccaa ggtggcggtc
     1021 acctcgaagc tggccccgcg cgtgctccgc aggcggtaca tctacaactt cccgccagcg
     1081 ccctactcgc cgtacaacat cggctcgcac acccaccccg gcttcggtcc tccctacgtc
     1141 gagctggact acatcctggc cgacccgaat aacgagtcgt tcgagtcgcc ggaccgcttc
     1201 gtgaacgggc agaacgcgat agccttcata caatcgtcgc ccgaatggaa gctggagtcc
     1261 agcaaagagg gcatcttgct cttcaagcgg caaccttagg cgggcagtgg ggctgtgata
     1321 taatctcgca cgaatgagag ctagtcaggc ggtaacatga gcaacgctgg agagtacaat
     1381 cctatagagc cggtgggtgt ggaactcgcg gagagcgaga accggctgta catcaactgg
     1441 agcgacggca ggaccacgca gtacgcggtg gactggctgc ggtggcagtg tccgtgcgcc
     1501 ctgtgcaggg gcgagatggg cgcgcccgga aggctcgact tcgtgaagga gcttagcccc
     1561 caggagacca ccatgcaggg cgcggagcag attggccgct acgccatcat gttcttctgg
     1621 gccgacgggc accacgacgg catctacacc tacgattggc tgcgccgcaa ctcgcaagac
     1681 gagcagagcg ctccgccggg cacccaggcc cccggttaga ctccgcagag gcgaaatctt
     1741 ggcaatagaa atccccacca gagagcaagc cagccgcacg ataccccccg cgtccagccc
     1801 tgagttggag gcgcggctcg tggaggctat cgagccgtgg ttgcagcaca tgaagtggcg
     1861 cgtggacttc gacgggtggc gggagcggcg catctggcag gaggagcacc aggaagccaa
     1921 cctgcgggac gtaaggtcgg tgctcggctc gcgagtgttg ggcgtgcggt tgctggacct
     1981 gggggcgggc atgggcggct tgtcggtggc cctgctgcgg gagttcgggg gcgcaggact
     2041 ggcggtgcag gccaccgact acaaccccag ctacgcgcaa atagcgcagc tgcgggcgaa
     2101 gcgttacgga ctggacctgc ccatcaccgt ggcggcgggc gaggcgctgc cctacgcgga
     2161 cggactgttc gacgtggtgc tctgcctgga cgtgctggaa cacgtgcagg acgtggaggc
     2221 agtgctgggt gaaacttaca gggtgctcaa gccgggcggc gtggtgctga ccaccgtgcc
     2281 caaccgccgc gccttccgcg acccccacta ccatttgccc ctgattaatt ggctgcccac
     2341 gccgctggcc gacaggatca tagagcgggt gggccgctcc aagagcgggg gacctctaca
     2401 ggaccggcag gagcactcgg agctaaatac ctacacctgg ggtaagtttg cccgcgtcgc
     2461 gtcgggtgtg ggcttccggg tgcgcgacca ggtgcggcac cgcatcctga gcggagaaat
     2521 ccggcagctt cacggttggc ggcgcgccct gctggcgggg gtacggaggc tagggctgct
     2581 ggacccgctg tatcgcgctt acaggtatgg ctggcagggc acataccagg tcatgctggt
     2641 aaagccccgg tgaagctcgc cctgtacaac ctgaccacaa ccacccgttg gggcggggtg
     2701 gagaccttcg tgtgggaggt ggccgcgcag atggcttcac gggggcacaa ggtaaccatc
     2761 ttcgggggcc gaggcaacgt cgagcgcagg cgcatagcgg gggtgcgcgt ccgcgtgctg
     2821 ccgtacatcg accgcgatgc ctggcgcaaa ctgccccttc tcagccgcca gtacggcctt
     2881 accaagctcc tcgaacggct gtcgatggcc ccctttgcgt tgcccgcgct tctggcgggg
     2941 aggttcgaca tcgtgcacat ccagaagccc tacgacctgc cggtgggcgc gcttgcccgg
     3001 ttgagcggcg cgaagctgct gttcggttgc cacggcaagg acttctggcc cggcgaccgg
     3061 ctgttcgtgc ggttcgccaa tgcctcggtg tcggccagcc ggttcaacgc cgaacaggtg
     3121 cgggcgcggt acggtatcga cccggtggtg gtgtacaacg gggtcgatct cacacgcttc
     3181 acgccggaag gtccgaacga cccccaggtg ctggcccagt acggccttgc gccttccagc
     3241 catgactcac ccaccatact gtacgcgggt cgcctggtgc gctggaaagg ggtcgaatat
     3301 ctcatcaagg cgctgacgga ggtcgtgccg gagaacaccg tgctgtgggt ggcgggagag
     3361 ggggagtacc ggcccgtgtt ggagcagatg gcgcgcgagg ttggagtgga cgacagggtg
     3421 cgcttcctgg ggaggctggg acaggacgac ctagccgccc tctaccgcaa ctgcgcaatg
     3481 ctggtagcca cgtcgttcgt caacgagacg ttcggcatgg cgctatgcga ggcgatggcc
     3541 tgcggcaccc cggtggtggc ttcggacttc ggcggcttcc gcgaggtggt ggtggagggg
     3601 gagaccgggt tccgcgtcag cccccaggac ccccgcgacc tcgccgccaa gatcacggcc
     3661 ctgctcgcca gcccccaact gcgcgacagg atggggcggg cgggcaggcg gcgcgtgctg
     3721 gagatgttca gctggaaggc ggtcggtgac aggctggagg aggtatacag gtgtctcgta
     3781 ccctcctcta cgtagcctac ccgatgcggc tcgacctggg cgcggccaac gcgatccaga
     3841 cctacagcac ggtacgcgag ctacagaagg tgctgcccgg cgtgcgcctg gtggtgcccc
     3901 gctggctgcg cgagagtagc gcgttcgagg agttgggcgc gttgcacctg ccgcgtccgg
     3961 ccgcgaacaa gctatcgaag tttctaccat gggcggggtg gtcctacctg gagcggacgg
     4021 tgtactcgtt catgctcctg gcgctgctgg tggcctggcg gctcacgggg aggggctacg
     4081 acgcgctgta cgtgagggat gccgtgtgcg ccgcctggct ggctctactg cggccactcc
     4141 acggcagccg cgtcatatac gaggtgcacg acctcgaagc ctcccaccct agcaaagcct
     4201 cgaagtggcc ccgcgctttc tggcggcggt tcctgccctg gttggaccgc gcggccctta
     4261 cgcgtagtga cgcgctggta tcgctgacag gggcgttcaa ggaatgggtg gtacgccggc
     4321 gattacggca accggggcat gtggccgtca tccccgatgc ctacgacccc gacgtatact
     4381 tcccggcgca caccgacacc gcgcgctccg agctgggcct gcctgtcgcc gtgcccataa
     4441 tcggctacac ggggctgacg ttcgcctaca ggggcctcga cttgctcgtg agggcctttg
     4501 cccaggtgcg gcggcaacac cctgaggctc tgctggtgct ggtcggcggc cggcagcacg
     4561 agatagagga gctaaagcag cttgtccgcg agtgccgcct cccggccgag agcgtgcgct
     4621 ttccggggca ggtgccgcaa cccgtctcgg cccagtacct gaacgcctcc gacgtgctgg
     4681 tcatcccgga gaccgttacg gttatgacgg cctcgccgct caagctgttc gagtacatgg
     4741 cggttggcaa acccatcgtg tgcaaggata tgcccgcctt gcgcgagata ctggatgggg
     4801 actcggcggt gttcttcgag gcgggcaata tcgaggcgct ggcggagggc atagcccgcg
     4861 tgctggacga cccgcaggcg ggtatgcggc tgggactgtc agccctggaa cgctctgccg
     4921 ggtacacgta ccgggcgcgg gccgagaaaa tcgcggaggt ggtgcggagt tgacggcggc
     4981 cgtgagaaca gttcccagag ccgagagcag ggaagtgacc gggcactggt ggtccgaccg
     5041 cgcgctcgtc gccctgctcg tgcttttcgg tctagtcgcc cccctggtga cacaccgcat
     5101 ctacgccagc gacgagatac agtacttcgc ctacacccac tcgctcttct tcgaccgtga
     5161 cttcgacttc ggcaacgagt acatgcactt ctacgcctcc aacccgcaga agttcgaggc
     5221 catctacacc gacctctacc tcaagaaaga agacctgacc gggctgccca tcaacgtcgc
     5281 gccgataggc actgggctgt tctggctgcc ctcttacgcg gtggcgcacc tgttcgtcac
     5341 ggcagcaaac ggcctgggcg cgaacatacc ggccgatggc tactcgcagc cctacataac
     5401 ggcaatctgc ctgacctcgt accttttggg ttgcgcgggg ttgctgctgt gctaccttat
     5461 ttcgcggcgg ctgttcggca agcgcgtctc tgcggttgga gtggcggtaa cgtggcttgc
     5521 gacgcccctg gtcttctaca cggtggtagc ccctccctgg tcccacgcca cgtctctgtt
     5581 cacggtgacg ctcttcctgt ggtactggct ccgcaccagg agggagtcgg ggcgcgggtg
     5641 gcgcgagtgg ttggtgttgg ggctgtgcgc ggggctgatg atgctagtgc gggagcagga
     5701 cgcgctcttt ctcatcgtgc cggggctgga agtggctgtg cggctcgtcg cccggtggcg
     5761 caagagcaag cttgcggacc ggggtgctct gatgagacct atcggtggcc tcatgctgat
     5821 gggtgtcacg gcggccatcg tgttcatccc gcagctggtt gcctacaggg ccataacggg
     5881 gcggtttggg ccgtcgaagg tggtgtcggg caagttcaca tggaccgctc ctaacgcgca
     5941 taacgtccta ttttcaccgg agcacgggct ggtgccgtgg acgcctgtcg tggtggttgc
     6001 cctgctgggg ctggtgctgc tctggcggcg cgacaggctg cttgcagggg cgttagctgt
     6061 ggctttcttc gtgcaggtgt atatcgccgg gtcgtttctt acctggcaga gcgccggttc
     6121 gttcgggcag cggcggttca tcaacagcac cgcgatattc gtgctggggc tggtggcggt
     6181 gctgtcgtgg gtgctggcgc gtggggtgcc acggtgggcg ctgggcgcgc tggcgctcct
     6241 gttcgtggcc tggaacggcg gcctgctcat acaatacgca ttgtggactt cggatcaacg
     6301 gcagggcctc gaatgggcga ccgtgttgca gggacagctc gaaatgcccg ccaaagccgc
     6361 ccgcttgctc tggacctaca tcaccgaccg agagcagttc tacagcgaaa cggggccgcg
     6421 ttgacggccc ggctccgacg gtgctagaat cacccctgcc agggccgcta ctcacgccct
     6481 ggactaccac actccacgcg agacaccata ttgatctccg atagactgct ccggtccgtt
     6541 tgctgcgtca acccggagtg catacaacag tggggcacag gcaccttgct cgccaggcag
     6601 gagcgcgcgg cgctgcaatg tcctcgctgc tccaccgtct acgcaatgaa ccccgcgggg
     6661 tacatcgagc taatgccgcc tcaacgcggg caacacacct ccctctacgt gtctcaggaa
     6721 gccgagttcg acgcggcgct cgattacaag cgcatcggca tcccgctgct aggggccgcc
     6781 gtccggcagc gtgccatccg cgagatgctc aagcctgcct catcggaccg cctgattgag
     6841 ctggggtgcg gcaacggcaa gttcgtcttc tggaacagga aagcggtaga gtgggccgtg
     6901 ggggtcgatc ccgccccgtt gttcgcacgc gaggcgctac agggcctcga cctgtgcctc
     6961 tccgatgccc gcgccctgcc cttcgaggac ggcgccttta ccgcagccat atcgattgac
     7021 gtgttggagc acctgcccct ggaggacata cgcgccttcc tgttggaggc caaccgcgtg
     7081 ctggacgagg gcggccgcct gttcctctat tccaacacgc gcgaaggctc ccggctggac
     7141 ttcatcgtgc ggagcgtgcg gcggctctcc cgctggctgg gggagagggg catgctggac
     7201 gacaggcgcg accgcctacg caagtcggac cacgtgaagg ccctcgccac ctatcccgac
     7261 cttgagaagg tgctggcaga gcacggcttc ggggtggagc gggtcgtctt ctggaacggg
     7321 ttgttccaga gcataatcga gaacctggtg gtcaaactcg gcgagtcggc gttgcggcgc
     7381 gctaagggta ggggcggggc ggtcctggac gaagcgcagg ggagcgcggg cggcgaggac
     7441 tccatgcggc gcgatgtgaa gcagcggctg gcgaggcgcg gccccgaata cagggccatg
     7501 atgctgctca cccgcctcat gtggctcgac ctggcgctct ttggaggctg gcgggcgggg
     7561 ccgtacttcg tgcttgcaag gcgcgtgcgg ggcgctccgg gcgtcggggt ggcggattga
     7621 gagtgctgta cgtggcaggg gcggtggaag tgttcggcac ctccggcggc tcgatacaca
     7681 tacaggaggt ggcgtgcggg ctgcgcgccc tggggcacga ggtgctggtg ctctccttgc
     7741 gaggcaaggc gggtgatggg agcgggacgt tggaatgtgg cgtgccggtg agggcgacgc
     7801 ggtggcgcaa ggaacttgcc ctgctgggat tgcccaaggt gaggcgtgcg atgcgagcct
     7861 tccgccccga cgtggtgatg gagcggttct acaacttcgc gggcgcgggt gtcctcgcgt
     7921 cccacaggca gggcataccg tccctgctgg aggtgaacgc ccccatgacg gacccgccgg
     7981 gcagcctcaa gacgcggctc gataggctgc tgcttggctc gatgaggcgc tgggccgtcg
     8041 cgcaagggcg gtggagcgat gccatagtca cgcccctggc ctccaccgtg ccgccggagg
     8101 tagggcggga gaagatacac gagctgccgt ggggggcgaa cgtggagcgt ttcgacccgg
     8161 cccgccgctt gcagggcgaa gagaggcaag ccctggcggc cgagttgggg ctggcgcttg
     8221 agcacccggt tgccgcgttc ctggggagct tccggcgctg gcacggcgtg gagcactttg
     8281 ccgaggcggc ccggcgcttg atcgatgggg gcgcgcctat ctcgttcttg gctgtgggcg
     8341 gcgggccgga gcttgagggc ctgcgggcgc gggtggcctc gtggggcctg ccgcccggcc
     8401 gcttcgtctt taccggcccc cagccgcacg agcgggtgcc ggaattgctc tccctggccg
     8461 atattggggt ggcaccattc gatgtggcgt cgcacgcgcc gctggcggag tacggcttct
     8521 actggtcgcc gctgaaggtg ttcgagtaca tggctatggc cctgccggtg gtcacgatag
     8581 atacgccgcc gttgaacgac atcgtgcgcg aggggcagga ggggctgctg ttccccacgg
     8641 gcgacatcga tggccttatt tcggcgttgg cgcggctggc gggcgatgcc ggactgcgcg
     8701 agaggctggg acagtcaggg cggcagaggg tggtcgccaa ctatagctgg caggcccatt
     8761 gcgccgcgct cgaccgcctc ctccggaaga tcgcccatgc ctgagcgcgc gcagcccggc
     8821 agcctcggcg tgtgcctgct caccgacgtc ttcccccccc acagcggcgg cagcggttgg
     8881 agcacctact acctgggtaa ggcgctttcg gagcgggggc acagggtcac tgtacttcga
     8941 cccgtgtacg acagcgggaa ggcaagaccc gcgcggcgca cttcgcgata cgaggccctt
     9001 gacgtagagg aggtgcttgt gccgcgacct cccggctggg cggccaaagc gggcataggt
     9061 agggcttggt cgcaacggct ggcggggcgg ctgatggaga aacgggcctc ggcgttggta
     9121 gaaaagggtg cggtcgatgt gctgcacggc cagcaccagg tgtcgggcgt ggcagcctca
     9181 agcgcggcct tcatgtcatg tcgcccggtg gtgtcggtgg tgaccgtgcg cgactactgg
     9241 ccgctttgcc cctccagcac gcggcttttc gaggatgccg ccggggaccc gttcgagtgc
     9301 cgcgattgtc accgcctgcg cgcctatctt agttgtgtcc gaagtcgggg cgtgagagac
     9361 acagccaagc tacccgtgag ttatacccgg tggctcaaca cactcgccgc tagcaggcaa
     9421 ctggcaaaga gcgacgctgt aatcgcggtc agcagctacg tcgggcgcga actggaccgt
     9481 agcagcaggg tgcccgcctc gcgcctgcgg gtgataccca acctcgtaga cctgcccagc
     9541 gtggggcgcg ctctggaagg tgcctggccg ctgaacgaca tatcccatga ggacaggttt
     9601 ctgctcttcg cgggcaagct ggacacgaac aaggccgcct ggcagttgcc caggttgctg
     9661 gcgcgtgccg gggtaagcct gcctgtggta atcgctggtg acgggccctg gcgtgataga
     9721 attgcgcggg aggcgtcgga actgggcctc gacttccact tctatgactg gctggataac
     9781 gacgcggtgc tccggctgat gaacaaggca gtggcgctgc tgtttccctc ggcctggcag
     9841 gagccattga gccgggtgct gctggaaggc tgcgccacgg gtgctgccat tgtggccctc
     9901 gacacgggtg gcaccgccga cgttgtcgag cacggcacaa gcggctggct tgcccacgac
     9961 ctggaagagt ttgccgccgg ggtgcgcgcg gttacatcag atgcgggact taacgccaga
    10021 ctgcggcgcg gcgcgcggga gcgggcggaa cgcaagttcg cggctcctgt cgtgtgcgcg
    10081 caggtcgagg ggctataccg ctcgttgctc gaacggaagg ggccacggca atgagggtcg
    10141 ccaactgcgt gcgcgctatc ttcccgcagc atggctatgg cgggctagaa cgggcggcga
    10201 ccgctttgac gcgcaatctc ctattgcggg gagtggacgt agacctcttt acgcgcgcgc
    10261 tgcctccggg gaagcccttg cgagtgccgc ctggggccaa gggcagagtt tccgtgcacc
    10321 cggtgcgcta cggcaggctg ccattgccgc ca
//
LOCUS       DASRWZ010000348         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1263227, whole genome shotgun sequence.
ACCESSION   DASRWZ010000348 DASRWZ010000000
VERSION     DASRWZ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263227"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..996)
                     /locus_tag="VFH60_05400"
     CDS             complement(73..996)
                     /locus_tag="VFH60_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6313251.1"
                     /translation="MPRIKVEKQDLVLANRGVEIRLSRDYTLLEEESMRRLREILEPY
                     PKASRFVELLVADAEVRANWDMADYMAVAKLHYNDHGEVHHKVVATVAAGLLKLLLEA
                     DVQPDVVSSGGGDADDAFVAVLGAALLHDIGNQVHREGHMMMSVVLAIPILDRLLAEF
                     YPEIEQRIEIRGFILHAIRTHDIDIRPLTMEAAIVAVADACDMTKGRSRYALDMGSIS
                     IHTIGGVSIERVVLEKGRKRPIRIRVDMVNSAGIFPVEEYLLPKINVGELAQHCELVV
                     TTEPQESTTDQRIIHTVEMEGKRFIAKGPEE"
     gene            1183..>2778
                     /locus_tag="VFH60_05405"
     CDS             1183..>2778
                     /locus_tag="VFH60_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HET6313252.1"
                     /translation="MGFLIPIALGLLALAIPIILFYMLRLRRQDVEVSSSFLWRRALQ
                     DRTANMPWNKLRRNLLLLLQLLLLLLLVLSLARPFFEAATVARGNMVVILDASASMQA
                     ADEAGGSSRFERARNEANALIDGMSGEARMSLVLAGPVPQVAVSASNNKSALRSALAG
                     MTPSNGGTDMAQAVTLGAATARQMGDATLVLISDGAFQGAETLPQVPGKSAYVNVGRS
                     GRNVGITALSLREAQGGPQLFASVANTGDQPATAILSIFIDGDLRDSRKLELASGAEQ
                     VVTLGDLPLDTHIAEASLQVDDVEADALGSDNRAWAVMSRPPSSNVLLVSEGNGFLEK
                     SLNLLPNVKLFKTTPAAYSPSGDFGLTVLDGVIPSPLPPGNLLIFAPPQSPLMVVTGT
                     LAYPAIGQVAVNDPLLRFVDLSGLHIAAAQRIVPPTWARVLVRTPAGDPLLLVGETEG
                     RRVAVVAFDLHKSDLPLQIAFPILVANLLDWLQPATSVDAPATLAAGDPISIRALPEA
                     DEVRVTLPEGASAERVVTLRPSGE"
BASE COUNT          439 a          879 c          901 g          559 t
ORIGIN      
        1 tcccttaggg cacccactta atcttggcgt ggtccttggc gttcttggcg tcttggcggt
       61 ttcgtctctt tattattcct ccgggccttt ggcgatgaag cgcttgcctt ccatttccac
      121 ggtgtggatg atgcgctggt cggtggtgct ctcttgaggc tcggtggtga ctaccagttc
      181 gcagtgctgc gccagctcgc ctacgttgat cttggggagc aggtactctt ccacggggaa
      241 gatgcccgcc gagttgacca tgtccaccct gatgcgtatc ggcctcttgc ggcctttttc
      301 cagcacgacg cgctctatcg acacgccgcc tatagtgtgg atgctgatgg aacccatgtc
      361 gagggcgtag cgggagcggc ccttggtcat atcgcaggcg tcggccacgg ccacaatcgc
      421 ggcctccatc gtgagggggc ggatgtcgat gtcgtgggtg cgtatggcgt gcaggatgaa
      481 gcctcgtatc tcgatgcgct gctctatttc ggggtagaac tcggccagga ggcggtcgag
      541 aatggggatg gcgagcacca cgctcatcat catatggcct tcgcggtgca cctggttgcc
      601 gatgtcgtgc agcagggcag cgccgagcac ggccacgaac gcatcgtcgg cgtcgccccc
      661 gccggaggag accacgtcgg gctgcacgtc cgcttccagc agcagcttga gcaggcccgc
      721 ggctaccgtg gcgactacct tgtggtgcac ctcgccatgg tcgttgtagt gcagcttggc
      781 taccgccatg tagtcggcca tgtcccagtt ggcccgcacc tcggcgtcgg ccaccagcaa
      841 ttcaacaaag cggctcgcct tcgggtacgg ctcaaggatc tcccgcagcc tccgcatcga
      901 ctcctcttcg agcagggtat agtccctgga gaggcgtatc tcgacgcctc tgttagccaa
      961 aaccaggtcc tgcttctcga ctttgatcct gggcatttgc ttcttccttt cggcactcaa
     1021 tctcacatgt aaggacagcg aaacccgcgc acactcagca cgttagggag taggatacag
     1081 catccgtgcc ggaattacga ccccttcggg gaattacgaa ttacgaatta caagatgttc
     1141 gccgtaattc gtaattcgta attccgacag gtacaatata gtatgggctt tctcatccct
     1201 atcgccctgg gcttgcttgc cctcgctata cctatcatcc tgttctacat gctgcggttg
     1261 cggcggcagg acgtggaggt gtcttcgtcg ttcctgtggc ggcgggcgct ccaggaccgc
     1321 acggccaaca tgccctggaa caagttgcgc cgcaacctgc tgcttctctt gcaattgctg
     1381 ctgctcctgc tgctggtgct gagcctggcc cggcccttct tcgaggcggc caccgtggcg
     1441 aggggcaata tggtggtcat actggatgct tccgcttcca tgcaggccgc tgatgaggcg
     1501 ggcggcagca gcaggttcga gcgggccagg aacgaggcca acgccctcat agacgggatg
     1561 tcgggcgagg ctcgcatgtc gctggtgctg gcggggccgg tgccgcaggt ggcggtgtcg
     1621 gcctccaaca acaagtcggc cctgcgctcc gccctggcgg gtatgacgcc ctccaacggg
     1681 ggcactgaca tggcgcaggc ggtgacgctg ggtgcggcca cggcgcggca gatgggcgac
     1741 gctacgttgg tgctcatctc ggacggggcg ttccagggtg cggaaacgct gccccaggtg
     1801 ccgggcaagt cggcttacgt gaacgtgggg cggtcagggc gcaatgtggg cataaccgcg
     1861 ctgtcgctac gggaggcgca gggcgggccg caactgttcg ccagcgtggc aaacacgggc
     1921 gaccaaccgg ccacggccat cctgtccatc ttcatagacg gcgacctgcg cgactctcgc
     1981 aagctggagc tggcgtcggg ggcggagcag gtggtcacgc tgggcgacct gcccctcgat
     2041 acgcacattg ccgaggcgag cctgcaagtg gacgacgtgg aggccgacgc gctcgggtcg
     2101 gacaaccgag cctgggccgt gatgtcgagg ccgccttcct ccaacgtgct gctggtgagc
     2161 gagggaaacg gtttcctgga gaagtcgctc aacctgctgc ccaacgtcaa gctcttcaag
     2221 accacgcccg ccgcttactc accctccggc gactttggcc tcaccgtact tgatggcgtg
     2281 ataccctcgc cgttgccgcc gggcaacctg ctcattttcg cgccccccca gtctcccctc
     2341 atggtagtga cgggcacgct ggcttacccg gcgatagggc aggtggcggt gaacgacccg
     2401 ctgctgcgct tcgtggactt gtcgggcctg cacatcgcgg cggcgcagag gatcgtgcct
     2461 cctacctggg cgcgcgtgct ggtgcgcacc cccgctggcg accccctttt gctagtgggc
     2521 gagacggagg ggaggcgggt agccgtggtg gcgtttgacc tgcataagtc ggacctgccc
     2581 ctgcaaatcg ccttccccat actcgtcgcc aacctgctcg actggctgca acccgccacc
     2641 tcggtggacg cacccgcgac cctggcggcg ggcgacccta tcagcatccg cgccctcccc
     2701 gaagccgatg aggtgcgcgt gacgctgccg gaaggggcga gcgcggagcg agtggtgacg
     2761 cttcgccctt ctggggag
//
LOCUS       DASRWZ010000349         7340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_988825, whole genome shotgun sequence.
ACCESSION   DASRWZ010000349 DASRWZ010000000
VERSION     DASRWZ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988825"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1203)
                     /locus_tag="VFH60_05410"
     CDS             complement(217..1203)
                     /locus_tag="VFH60_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET6313253.1"
                     /translation="MHNVITGGAGFIGSHLSDTLLGQGHRVTAVDNFLTGNRANIAHL
                     AGHPDFRVIEQDITVPVGDEWHRDLGHVDAIFHLASPASPADFTTMPLAIAIINSQGT
                     HNVLEWARRHNARYLITSTSEAYGDPDVHPQSEEYRGNVSTTGPRACYDEGKRFAEAL
                     TSIYANEYDVDARIVRIFNTYGPRMNPHDGRSVPNFITQAIRGEDLTIYGDGTQTRSY
                     CYVSDMVDGLIAVMFAPPETRGMVFNVGNPDEREIRNLAEVIRDACGSSSEIVYRPAL
                     RDDPVRRCPDITRVSTMLGWRPTVTLQEGLGRTVPWFRERLAQEEAAAGVAS"
     gene            complement(1299..4691)
                     /locus_tag="VFH60_05415"
     CDS             complement(1299..4691)
                     /locus_tag="VFH60_05415"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET6313254.1"
                     /translation="MRKWLAPVWSNPVARCLLALLLFAASLGGLSLTAYGDQLENGGV
                     AYERPPQPIPYTDVNPLGVNTFLEKEADPENVVRTLDMVQAGGFKWIRQVFPWNDIEI
                     SGKGNFTDTRHPEQVRSSWEKYDFIVDEAARRDINIIARLDSPPWWARFPGDDLEKYH
                     KGPPAKDEDYADFARAVAERYKGKVKYFQIWNEPNLEGEWGGYAIDPEWYTRLLKAAY
                     IAVKEANPEAVVISAALAPTTANSLRNLNDVLFLEGMYQAGARPYFDIFSTMLYGLGQ
                     PPDERRVDLKRLSFSRPILLHEVMERYGDGNKAIWISEYNWVSLPPGWEEEKANAHEE
                     EKQFWGTNIWGASVDEETQGRWLVEGYERVRREWPWLGVMNVWFLRDPTADRRQPASW
                     FAIVQPDFTPRPAFMALQNYSATLGPERLPAQKPPWNAFGFPFLYGVFGLMSVASAAW
                     GLSNLGRWAGAALNIPRDRYRETAQELARNGVVVVLMVLLVGLYFGTQSVPLALLVLA
                     CWGLLAFIKPSTGLAAVAFTIPFFWQPKFLGSNKLPLAETLLLVTFGAVVARHVVLRF
                     LPRVAAWLRMYDYQPGQTEMDLRRWMALQAPIPSPNDRELSIHERATEPNGWYGANGN
                     GWTGSALVGPGSLKSTLPLEDEEDDEIEPAHWPSRPRPVRFRYFSDPLPRAAFVFPAQ
                     PQTWAQMRAYNNQAKAAATAELEAEAAASLAVPPAKATQPIERLTLPVLDEQPLPVPV
                     AAPDTQALTSWQAQLWARFKAWDQEDAFAPPAVLLLLLGLLSLLWVADPAFLTDSARQ
                     FRWVIIEPVLYYFLLTEVIKNRRSLLRVADFFMAGAVIVAVLGLVQYVQGSGTLVVEG
                     VNRITGVYQHPNNLGLFMGRVMPVAACLALFLPWGWRKLVYASIALPLAATLLLTFSR
                     GAWLGAAAGMVVALAVGFRYRQGRIAGPVTRRTWGIAAGVVVGLVLVGAVAYPYLPER
                     VKSLGSGFIRLNIWESALHMGADHPILGVGLDQFINQYQAKYIAPEQRDDPATPQVEG
                     EGYTSHPHNIVLDYWLNLGIMGLFVLLWLLWKYFRVAINRVKAFTGTAGADPVGRALA
                     LGLLALMVDFLVHGLIDNSYFLMDLALVFWLSCAMLQIMRRT"
     gene            complement(4700..6964)
                     /locus_tag="VFH60_05420"
     CDS             complement(4700..6964)
                     /locus_tag="VFH60_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET6313255.1"
                     /translation="MAKETTPRGGGSGKGAHASNASNAANARRGDGHAHSKSQAPLDG
                     KATGANGKVAGTDGSGNSSRTQAPGTVMLKTQGLTTASARARTLSSDGDGSHGSHGSP
                     ATEVLPVAASALNTGPSLLGANGIPLDRRSLTTDELTSIAQARRKLNLRALIVALSLL
                     LVLITVFYSPLFDPVSGQPPRMVSPVPMAEVQPYGVNTFLHKEVEDWKRDKTLERAQD
                     MGAGWIRQQFPWAEIEYRQDPDNPFWDVKNNQNAWDKFDRIVAAAERYDLRVIARIDN
                     APEWSHPGTSTLQSPPDATHLPDFANFIKVFVERYRGRIAAIQVWNEPNLTGEWAVRD
                     PAYPNGNRPVNPREYVDMLKAVYEAAKSADPNMIVLAAPLSINNETLAYAGNLNELDY
                     LQGMYDAGARPYFDVMSANAYGKEFPPEDPPSRDKLNFRRVELLREVMERNGDGSKAV
                     WFNEYGWNSSPADGTVDEKTVRWGRVTSEQQAEYTVRGIEYAREHWPWAGVFTIWYLR
                     QVGDISETNSEYYFGLVSFDFVVGDTYKAVQKAVQASEHVAMPGQWGPASAPVQASTN
                     WRLKLSPAVPGGMYVAPTTIGDTLDMQFRGTDVKLMLVPPAGTDVISGTAINARYYVT
                     VDGSSDNVASDLPRDASGQAYIEVPGSGQATEVTLARGLGASFRTGQHNLQIKVGQDE
                     RAAGGRSEKTAGLHAPARQYVDLPGIGAVTVEAHSSYWLFGAVALLLLASIAFCAWAL
                     TRGGTWGPALPYGR"
BASE COUNT         1232 a         2331 c         2422 g         1355 t
ORIGIN      
        1 tactgaaaag ggtaacctcc cggtgaggga cgagattctt cgccgcgctc agaatgacaa
       61 ggaccaccct tgttcgcgta tttcgtggag gttgcagata gaccctacgc tcatcaagag
      121 agtccccgca ggggactttg ccacttccca gcccgtggct ttagccacag ggcgtcatgc
      181 agggcgtctc cacagggcgt aagtaccttc tagcccctaa gaagccaccc ccgcggctgc
      241 ttcctcctgc gccagccgct cgcggaacca ggggaccgtc ctgccgaggc cctcctgcaa
      301 cgtcaccgtg ggccgccagc cgagcatggt gctgacgcgc gtgatgtcgg ggcagcgccg
      361 cacggggtcg tccctgaggg cggggcggta gacaatctcg gaggacgatc cgcaggcgtc
      421 gcggatcacc tcggccaggt tgcgtatctc ccgttcgtcg gggttgccca cgttgaagac
      481 catgccgcgc gtctccggcg gggcgaacat caccgcgatt agaccgtcca ccatgtcgct
      541 cacgtagcag taggagcggg tctgcgtgcc gtcgccgtag atggtcaggt cttccccgcg
      601 tatcgcctgg gtgatgaagt tgggcaccga gcgcccgtcg tgcgggttca tacgcgggcc
      661 gtaggtgttg aagatgcgga cgattcgggc gtccacgtcg tactcgttgg cgtagatgga
      721 ggtgagggcc tccgcgaaac gcttgccctc gtcgtagcag gcgcgagggc cggtggtgct
      781 cacgttgccc cggtactcct cgctctgcgg gtgcacgtcg gggtcgccgt acgcctccga
      841 ggtggaggta atgaggtacc tggcgttgtg ccgtcgcgcc cattccagca cattgtgggt
      901 gccctgcgag ttgatgatgg cgatggcgag gggcatcgtg gtgaaatcgg cgggggaggc
      961 ggggcttgcc aggtggaaaa tagcgtccac atggcccagg tcgcggtgcc actcgtcgcc
     1021 caccggcacg gtgatgtcct gctcgatgac cctgaaatcg gggtggcccg ccaggtgcgc
     1081 gatgttggcc ctgttgcccg tgaggaagtt atcgacggcc gtgaccctgt gcccctgccc
     1141 cagcagagta tcgctcaggt gcgacccgat aaaccccgcg cctccggtaa tgacgttgtg
     1201 caatagtatc cctccacttg gtatttcgga tttcgacttg cggatttgct cggatatcta
     1261 ctaaattcga aatccgaaat ccgaaattcc aaatctcttc acgttcttcg cattatctgc
     1321 aacattgcgc aactgagcca gaagacgagg gccaggtcca tcaggaaata ggagttgtct
     1381 atcaggccgt ggaccaggaa gtctaccatt aaagcgagca ggccgagagc cagcgcgcgc
     1441 ccgaccgggt cggcccctgc cgtgccggtg aaagccttca cccggttgat ggctacccgg
     1501 aagtatttcc agaggagcca caggagcaca aacaggccca ttatacccaa attgagccag
     1561 tagtccaaaa ctatgttgtg cgggtgcgag gtgtagcctt ccccctcgac ctggggggtg
     1621 gcggggtcgt ctcgctgttc gggggctatg tacttcgcct ggtactggtt gatgaactgg
     1681 tcgaggccca cgcccaggat ggggtggtcg gcccccatgt ggagggcgct ttcccagatg
     1741 ttcaagcgta tgaagccact gcccagcgac ttgacgcgct ccggcaagta gggataggcc
     1801 accgcgccca caagtaccaa cccaaccaca acgcccgccg ctatgcccca cgttcgccgc
     1861 gttacaggcc cggcgatcct accctgccgg tagcggaagc ccaccgcgag ggccaccacc
     1921 atgcccgccg ccgcacccag ccacgcgccc cgcgagaagg tgagcaggag ggtcgccgcc
     1981 aggggcagtg cgatgctcgc gtacaccagc ttgcgccaac cccacggcag gaacaacgcc
     2041 aggcacgcgg cgacgggcat cacccggccc atgaacagcc ccaggttgtt cgggtgctgg
     2101 tagacgccgg tgatgcggtt cacaccctcc acgaccagcg tgccggagcc ttgcacgtat
     2161 tgcaccaggc ccagcaccgc cacaatgacg gccccggcca tgaagaaatc ggccacgcgt
     2221 aacagcgagc gcctgttctt gatgacttcg gtcagcagga agtaatagag caccggctcg
     2281 attatcaccc agcggaactg ccgggcgctg tcggtcagga aagcggggtc ggccacccac
     2341 agcagcgaca ggaggcccaa caggagcagc aacacggcgg gcggcgcgaa cgcgtcctcc
     2401 tggtcccacg ccttgaaccg cgcccatagc tgcgcctgcc acgacgtaag ggcttgagtg
     2461 tctggggcgg ctacgggcac gggaagcggt tgctcgtcga gcaccggcag cgtcagacgc
     2521 tctattggct gtgtggcttt ggcgggcggc accgctaagc ttgcggcggc ttctgcctcc
     2581 agctccgccg tagccgctgc cttagcctgg ttgttgtaag cccgcatctg cgcccaggtc
     2641 tgcggctggg ctggaaacac gaaagccgcc ctggggaggg ggtcggagaa gtagcggaac
     2701 ctgaccggcc tggggcgcga gggccagtgc gcgggctcta tctcgtcgtc ctcttcgtct
     2761 tctaggggca gggtgctctt gagcgagccg ggtcccacaa gggccgagcc ggtccagccg
     2821 ttgccgtttg cgccgtacca gccgttaggc tcggtcgcgc gctcgtggat gcttagctcc
     2881 cggtcgttgg ggctgggtat tggggcttgc agcgccatcc agcgtctgag gtccatctcg
     2941 gtctggccgg gctggtagtc gtacatgcgc agccaggcgg cgacgcgagg caggaagcgc
     3001 aacacgacgt ggcgggccac caccgcaccg aacgtgacga gcagcagcgt ctcggccagg
     3061 ggcagcttgt tcgaccccag gaacttgggt tgccagaaga agggtatggt gaaggccacc
     3121 gcggccagcc ccgtcgatgg cttgatgaaa gccaacagcc cccagcacgc cagcacgagc
     3181 agggccagcg gtacgctctg ggtgccgaaa tacagcccca ccagcagcac catgagcacg
     3241 accaccacgc cgttgcgcgc cagctcctgc gccgtctccc ggtagcggtc gcgtgggatg
     3301 ttgagggcgg ccccggccca cctgcccagg ttgctcagcc cccaggcggc cgaggccaca
     3361 gacataagcc cgaacacgcc atagaggaag gggaagccaa acgcgttcca gggcggcttc
     3421 tgcgccggga gccgctccgg tccgagggtt gcgctgtagt tttgcagggc catgaatgca
     3481 gggcgcgggg taaaatcggg ctgcacgatg gcgaaccacg aggcgggttg tctccggtcg
     3541 gcggtggggt cgcgaaggaa ccagacgttc atcaccccca gccagggcca ttcgcggcgc
     3601 acccgttcgt agccctcgac cagccagcgg ccctgcgtct cctcgtccac gctcgcgccc
     3661 cagatgttgg tgccccagaa ctgcttctcc tcctcgtggg cgttggcctt ctcctcctcc
     3721 cagcccggcg gcaggctgac ccagttgtat tcgctgatcc agatggcctt gttgccgtcg
     3781 ccgtagcgct ccatcacctc gtgcagcagt atggggcgag agaagctgag ccgcttcagg
     3841 tccacccggc gctcgtcggg aggctggccc agaccgtaaa gcatggtgga gaagatatcg
     3901 aagtagggcc tggccccggc ctggtacatc ccttccagga aaagcacgtc gttgaggttg
     3961 cgcaacgagt tcgcggtggt gggggcgagg gccgccgaga tgaccacagc ctcggggttg
     4021 gcctctttca ccgcgatgta agcagccttt agcagccggg tgtaccattc cgggtcaatg
     4081 gcgtacccgc cccattcccc ctccaggttg ggctcgttcc atatctggaa gtacttgacc
     4141 ttgcccttat accgctcggc cacggccctg gcgaagtcgg cgtagtcctc gtctttggcg
     4201 ggggggcctt tgtggtactt ttccaggtcg tcgccgggga agcgcgccca ccaggggggg
     4261 ctgtcgaggc gggcgatgat gttgatatcg cgcctggccg cctcgtccac gatgaagtcg
     4321 tacttctccc aggagctacg cacctgttcg gggtgccgcg tgtcggtgaa gttgcccttg
     4381 ccgcttatct cgatgtcgtt ccaggggaag acctgccgta tccacttgaa gccgcccgcc
     4441 tgcaccatat cgagggtgcg cacgacgttc tcagggtcgg cctccttctc caggaaggta
     4501 tttacaccca ggggattgac gtccgtgtaa gggatgggtt gcgggggccg ctcgtaggcg
     4561 acgccgccat tttcaagctg gtcgccgtaa gcggtgaggg agaggccgcc gagggaggcg
     4621 gcaaagagga gcagagctag caggcagcgg gcgaccgggt tagaccagac gggcgccagc
     4681 catttgcgca cgcgtcgcat caccgaccat aaggcagggc cggaccccac gtcccgcccc
     4741 tggtgagcgc ccaggcgcag aaggcgatgc tcgccaggag gaggagcgcc acggccccga
     4801 acagccagta ggagctatgc gcctccaccg tgaccgcgcc gatgccgggt agatcgacgt
     4861 actgccgggc gggcgcgtgc agccctgccg tcttctcgct cctgccaccc gcggcccgct
     4921 cgtcctgccc cacctttatt tgcaggttgt gctggcccgt gcggaagctg gcccccaggc
     4981 cgcgggccag cgtcacctcg gtggcctgcc cgctccccgg cacctctatg tacgcctgcc
     5041 ccgaggcatc gcgaggcagg tcggacgcta cgttgtcgct gctgccgtcc acggtgacgt
     5101 aatagcgcgc gttgatggcg gtgcccgaaa tcacgtcggt gccggcgggc gggacgagca
     5161 ttagcttgac gtcggtgccc ctgaactgca tatccagcgt gtcgcctatg gtggtgggcg
     5221 ctacgtacat gccgcccggc acggcggggc tgagcttgag ccgccagttg gtggacgcct
     5281 gcaccggggc ggaagcaggc ccccattgtc cgggcatcgc tacgtgctcg ctggcctgca
     5341 ccgctttttg cacggccttg taggtgtccc cgaccacgaa atcgaagctc accagcccga
     5401 aatagtactc gctgttggtc tcgctgatgt cgcctacctg cctcaagtac cagatcgtga
     5461 acaccccggc ccagggccag tgctcccggg cgtactcaat gccacgcact gtatattcgg
     5521 cctgctgctc gctcgtcacg cggccccagc gcaccgtctt ttcgtctacc gtgccgtcgg
     5581 cgggcgagga gttccaaccg tactcgttga accataccgc cttgctgccg tcgccgttgc
     5641 gctccatcac ctcgcgcagt agctccacgc ggcggaagtt gagcttgtcc ctggagggcg
     5701 ggtcttcggg cgggaactcc ttgccgtagg cgttggccga catcacgtcg aagtagggcc
     5761 gcgcccccgc gtcgtacatg ccctgcaagt agtccagctc gttgaggttg cccgcgtagg
     5821 caagcgtctc gttgttgatg gagagggggg ccgccagcac gatcatgttg gggtcggcgc
     5881 tcttggccgc ttcatagacc gccttgagca tgtccacata ttcgcgcggg ttgacggggc
     5941 ggttgccgtt gggataagcc gggtcgcgca cggcccattc gcccgtgagg ttcggctcgt
     6001 tccagacctg tatcgcggcg atgcgccccc tgtagcgctc cacgaatacc ttgatgaagt
     6061 tggcgaagtc gggcaggtga gtggcgtcgg gcggcgattg gagggtgctg gtgccggggt
     6121 gcgaccactc gggcgcgttg tcgatacggg cgatcacgcg taggtcataa cgctcggcgg
     6181 cggccacgat gcggtcgaac ttgtcccagg cgttctggtt gttctttacg tcccagaagg
     6241 ggttgtcggg gtcctgcctg tactcgattt cggcccaggg gaactgctgg cgtatccaac
     6301 ccgcgcccat gtcctgcgcg cgctccaacg tcttgtcccg cttccagtcc tccacctctt
     6361 tgtgcaggaa ggtattgacc ccgtaaggct gcacctcggc catgggcacg ggcgagacca
     6421 tgcgcggcgg ctgccccgaa accgggtcaa acaacggcga gtagaatacg gtaatgagga
     6481 cgagcaggag ggacagggcc acgatcagcg cccgcaggtt gagcttgcgg cgtgcctggg
     6541 cgatgctggt aagctcgtcg gtggtgagcg accggcggtc gagggggatg ccgttggcac
     6601 ccaggagcga agggccggtg ttcagggcgg acgcggctac gggcaggact tcggtggcgg
     6661 ggctgccgtg gctgccgtga ctgccgtcgc cgtccgagga cagggtgcga gcgcgggcgg
     6721 acgcagtggt gaggccctgg gttttgagca taacggtacc gggtgcctga gtacggctgc
     6781 tattgccgct gccgtcagta cccgctactt tgccgtttgc gcctgtggcc ttgccgtcta
     6841 gcggagcttg cgatttagag tgtgcgtgtc cgtccccgcg tctggcgttg gcggcgttcg
     6901 aggcgttaga agcgtgcgcg cccttacctg acccgccgcc ccgtggcgtg gtctccttcg
     6961 ccaaaataca ggctcctcct gccggtgcct ctcctggccc gattaaaacc gatcttcgag
     7021 cgcggcccga cagagtgaaa tcgccctgcc tcgcgtttct aagcgcgatg cggcctttat
     7081 gctcaaacca cattatacac attgagcaat acacaggcaa acaaaagcac cctttatgtt
     7141 ataatacagc ttgcccgaat cgcgggcgct ttttggaata tccattctac attcgttaac
     7201 gaggaccgag gcggcgggtt gtagacgtga cgagtgacga gtgacgacgc ttacaaaggc
     7261 tggcacgtgg gtgtaccaca ctcggtgcgg acggactctc tcccgccttt gtcattctga
     7321 gcgtagcgaa gaatctcgtc
//
LOCUS       DASRWZ010000350         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_98361,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000350 DASRWZ010000000
VERSION     DASRWZ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98361"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="VFH60_05425"
     CDS             complement(<1..1519)
                     /locus_tag="VFH60_05425"
                     /inference="COORDINATES: protein motif:HMM:NF037017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET6313256.1"
                     /translation="MSQNASSRTTTRRARSIVRQARRTIRLKAPAWLNIALALMLLLS
                     LLPASSYAAGSLAPASSVRVLGVTAGVGMTQGEVEGAAVIAVTFSEPMNLRSAEAAFS
                     INPSVPGTFAWQKATLLFTPRQALNAGTTYTVTVGKSARATRGPALAAPFSYSFTTAP
                     PPTVLRTLPSAGASDVPTDTVLTLSFDRLMSPLDSLSNTPDAARWVTISPTPRGRWFW
                     LGTATVGFRPEGGFLPGTEYSVEVKPGWPDAAGVPLQSGLSFRFSTLAPAVVGSSPAD
                     TALSQDLDVPVVARFNQPVDAESVQSAFSLAPEGGGAPVSGTYSWSPDSKVMTFTPAS
                     LLAFDTRYVATIAGPVKPLNAASGQPKATSRTWRFTTTIQTQVSYSDPKGIIGIERAS
                     DLSITFNNPLKPGQDFASFLSITPQPEEIGALRADPENGRIYVNKLRLLPDTAYMVEV
                     KPGLLDKWGFPVKSESWEVRTTPRAPGISITGGSFQPIHPDGPSQVGIQVANLTKV"
     gene            complement(1602..>2056)
                     /locus_tag="VFH60_05430"
     CDS             complement(1602..>2056)
                     /locus_tag="VFH60_05430"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HET6313257.1"
                     /translation="SVVDAFVAKLNPQGTELVFSTLLGGSSADRGGDIGLDGAGNVYV
                     AGSTTSPDFPLQNPLQDEYSDADYGMATDSFIAKLTPDGTSLFYSTYFGAEGFDSALG
                     FDVGRDGSVYLAGSVDSPSPGFPLAGKPYQSTNLGIRSAFIAKISDAP"
BASE COUNT          361 a          603 c          731 g          361 t
ORIGIN      
        1 gcaccttcgt gaggtttgcc acctgtatgc ctacctggct ggggccgtca gggtggatgg
       61 gctggaagct cccgccggtt atagatatac cgggggcacg cggggtcgtt cgcacctccc
      121 agctctcgct cttgaccgga aagccccact tgtcgagcag gcccggcttc acctcaacca
      181 tataggcggt gtcgggcagc aggcgcaact tattgacgta gatgcggccg ttctcggggt
      241 cggcccgcaa cgcgcctatc tcttccggct gaggcgtgat actcaagaac gaagcaaagt
      301 cctggccggg ctttaggggg ttgttgaaag taatagagag atcgctggcc cgctcgatgc
      361 cgataatacc ctttggatcg ctgtacgaca cctgggtctg gatagtcgtg gtgaaacgcc
      421 aggtcctgga cgtggccttt ggctggcccg aagcggcatt tagcggcttg acggggccgg
      481 cgatggtcgc cacgtacctg gtatcgaagg ccagcaggct ggctggggtg aacgtcatga
      541 ctttgctatc aggcgaccag ctatacgtac cgcttacggg cgcaccgccg ccttcggggg
      601 cgagggaaaa ggcgctctgc acggattcgg cgtctaccgg ctggttgaag cgcgcgacga
      661 cgggtacgtc caggtcctgc gatagggctg tatcggccgg agagctaccg accacggcgg
      721 gtgccagcgt gctgaagcgg aagctcaggc ccgactgaag gggcactccc gccgcgtcgg
      781 gccagcccgg tttcacctcg accgagtact cggtgccggg gaggaagccc ccttcgggcc
      841 ggaaccccac cgtggccgtg cccagccaga accagcggcc gcgcggggta gggcttatag
      901 tgacccagcg ggcggcgtcc ggcgtgttgc tgagcgagtc gaggggcgac atgaggcggt
      961 cgaacgacag cgtcaacacg gtatcggtgg gcacgtcgct cgcgcccgcg gagggcagag
     1021 tgcgcaggac cgtaggcggg ggcgcggtgg taaagctgta tgagaaaggg gccgcgaggg
     1081 caggtccgcg ggtggcgcgc gcgctcttgc cgaccgttac ggtgtaggta gtgcccgcat
     1141 tgagcgcctg cctgggggtg aagaggaggg tggctttctg ccaggcgaag gtgcccggca
     1201 cggaaggatt gatggagaag gcggcctccg cgctcctcaa gttcatcggc tcggagaagg
     1261 taacggcgat aacggcggcc ccttctacct ctccctgggt catgcccacg cccgccgtaa
     1321 cgcccagcac gcgcacgctg ctggcagggg ccaggctgcc cgccgcgtag ctggaggccg
     1381 gaagcaaact caggagcagc ataagcgcga gggcgatgtt gagccaggcg ggagccttca
     1441 atctgatggt gcgccgggcc tgccgcacaa tgctcctggc tcgtcgggtc gtggttcggg
     1501 aacttgcatt ctgcgacatg gtaggtgctc cacttccgtt cagctaaggg tgttgtcggg
     1561 taaggtcgag ataacggttg gtgtgccgcc tgaggcggac gctagggcgc gtcagagatc
     1621 ttggcgataa aggcgctcct gatccccagg ttggtcgatt ggtacggctt gcccgccagg
     1681 gggaagccgg gcgaaggcga gtccacgctg cccgcgaggt acacactccc gtcccggcct
     1741 acgtcaaagc cgagcgcgct atcgaaaccc tcggccccaa agtaggtcga gtagaacagg
     1801 gaggtgccgt cgggcgtcag cttggcgatg aaagagtcgg tcgccatgcc gtaatcggca
     1861 tcgctgtact cgtcctgcaa agggttctgc agcgggaagt cgggggaagt ggtgctgcct
     1921 gcgacgtaga cgttgcctgc gccgtccaaa ccgatgtcgc cgccccggtc cgcgctgctt
     1981 ccaccaagca gggtggagaa aaccagctcc gtaccctggg gattcagttt ggcgacgaat
     2041 gcgtcaacaa cgctgt
//
LOCUS       DASRWZ010000351         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_920958, whole genome shotgun sequence.
ACCESSION   DASRWZ010000351 DASRWZ010000000
VERSION     DASRWZ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920958"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /gene="infB"
                     /locus_tag="VFH60_05435"
     CDS             complement(<1..1385)
                     /gene="infB"
                     /locus_tag="VFH60_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET6313258.1"
                     /translation="MRGGGRGFGGIGRLGTLERPTPKVELPPFMTVKEMADEMGVNPT
                     GIIRELMKHNVLATIYQQIDYDSAAIVAIGMGYEVSEAIPEVEDDLGEALTNQQGATS
                     TARLAPRPPVVTIMGHVDHGKTKLLDTIRKTNVVATEAGGITQHIGAYQVELQGKKIA
                     FLDTPGHEAFTAMRARGAQATDIVILVVAADDGVMPQTLEALSHAKAANVPIVVAINK
                     VDKEDANPERVKTQLSQQELVPADWGGETEFIEVSARTGDGIQSLLETILTVADLAEF
                     KATPDRPATGVILEAEMDRNRGPLARALIQNGTLNLRDYVVAGATWGRVKAMQDDKGR
                     KLRKAEPSTPVELLGLTAVPQAGDTLLVAPDEQTAKQIAEQRARARRMEESTQGLKTV
                     SLDDLFAQIQAGAIKELRIILKADVQGSLEAITQTLAKLSTDKVKVNVLHKATGGITE
                     SDVALAYASSA"
BASE COUNT          218 a          520 c          468 g          318 t
ORIGIN      
        1 atggcgcttg aagcataggc gagcgccacg tccgactcgg tgatgcctcc cgtcgccttg
       61 tgcaacacgt tcaccttcac cttatccgta gacagcttgg caagcgtctg cgtgatagcc
      121 tccaacgagc cttgcacgtc ggccttcagg attatgcgta gctccttgat agcgcccgcc
      181 tgtatctggg cgaagaggtc gtccaggcta acggtcttga ggccctgagt gctctcctcc
      241 atgcggcggg cgcgggcgcg ctgctcggca atctgcttgg cagtctgctc gtccggggcc
      301 acaaggaggg tatcgccagc ctgcggcaca gccgtcagtc ccaacaactc aacgggggta
      361 gacggctctg ctttgcgaag cttgcgcccc ttgtcgtcct gcatggcctt cacgcggccc
      421 caggttgccc cggccaccac gtagtcgcgc aggttgagcg tgccgttctg gataagcgcc
      481 ctggcgagcg gaccccggtt gcggtccatc tcggcttcca ggatgacacc tgtggcgggc
      541 ctgtcgggtg tcgccttgaa ctcggcaagg tcggccaccg tcaggatcgt ctctagcagg
      601 ctctggatgc cgtcgccggt gcgggccgac acctcgatga actcggtctc gccgccccaa
      661 tcggcgggca ccaattcttg ctgtgagagt tgcgtcttca cgcgctcagg gttggcgtct
      721 tccttgtcta ccttgtttat agccacgacg ataggcacgt tcgcggcctt cgcatgcgaa
      781 agcgcctcaa gcgtctgggg catcacgccg tcgtccgccg ccaccaccag gataactatg
      841 tctgtggcct gcgctccacg cgcgcgcatg gcggtgaacg cctcgtggcc cggcgtgtcg
      901 aggaaggcta tcttcttgcc ctgcaactcg acctggtatg cgcctatgtg ctgcgtgata
      961 ccgcccgcct cggtagcgac cacgttggtc ttgcgtatgg tatcgagcag cttggtcttg
     1021 ccgtggtcta cgtggcccat gatggtcacg acgggcggcc tcggcgcgag cctggcggtg
     1081 ctcgtcgcgc cctgctggtt ggtcagcgcc tcgcccaggt cgtcctcgac ttcgggtatg
     1141 gcctccgaca cctcatagcc catgccgatg gcgacgatgg ctgcggaatc gtaatctatc
     1201 tgctggtaga tggtggccag cacgttatgc ttcataagct cgcggatgat gccggtgggg
     1261 tttacgccca tctcgtccgc catctccttc actgtcatga agggaggcag ctcaactttg
     1321 ggggtggggc gttccagggt gcccaggcga cctatgccac cgaagcccct gccaccgccg
     1381 cgcacacccg cgcctgccgt gcccggccct cgattgcctc cacccgcgcc tccgcgacct
     1441 ccattatttc cgcgacctcc gaagccgcct ccggggcgac ctcctgtgtt gcggaacccg
     1501 ccgccgggtc gagccgcgcc accc
//
LOCUS       DASRWZ010000352         1875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1195207, whole genome shotgun sequence.
ACCESSION   DASRWZ010000352 DASRWZ010000000
VERSION     DASRWZ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195207"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFH60_05440"
     CDS             complement(<1..549)
                     /locus_tag="VFH60_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313259.1"
                     /translation="MPANLTLRRLLTHTAVVLLALALCVGVAQAGLPTRQGPQGLTSE
                     VGADTQQSDTSRIVQVQWGGQTRPFDCSTIAVRGIDRQTNARAARILAACGEASGDPY
                     KSGDAFPNAVEQFRNSFRYGGTDRTVNNYAADVYPKVTQSESTVWANGSTVVVHYNSS
                     TDAPNCYAGISYSTDGGATFTQI"
     gene            906..1646
                     /locus_tag="VFH60_05445"
     CDS             906..1646
                     /locus_tag="VFH60_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313260.1"
                     /translation="MVDLSEVLRHKGEPRTDHYVEVPTPLGKLRLDLLGEVGAALAQA
                     GMTPEAQQATAKAIDAARAKLATGDGEGAKRLLLDAAKQYPYTANVYGMLYDVFTAEG
                     DLPEAEFCMKQAIALGPDFRNLTFLARNLGRQGRLEEAATIQEFLWQTRSEAAPEQAL
                     DAIHDYLVTLGRLQQPQAMMDVSMRAMQEHGSETTLVYQYVFALVLSNQQEAAREHLR
                     RVLPQLAADDPLYPRFVQMSDYLGGRLT"
     gene            complement(1657..>1875)
                     /locus_tag="VFH60_05450"
     CDS             complement(1657..>1875)
                     /locus_tag="VFH60_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313261.1"
                     /translation="PLRDITPQASGTAEANQPVGDVFIPGGPGWDESQYKDGDMVVAF
                     GRLLGPGDARVMCPGGTHYYVERVQPNP"
BASE COUNT          346 a          569 c          605 g          355 t
ORIGIN      
        1 aatttgggtg aacgtggccc cgccatcggt ggagtaggag atgcccgcgt agcagttggg
       61 ggcatcggtg gagctgttgt aatgcaccac aaccgtgctg ccgttggccc acacggtgct
      121 ctcggactgg gtcaccttgg ggtagacgtc ggcggcgtag ttgttgacgg tgcggtcggt
      181 cccgccgtat ctgaagctgt tcctgaactg ctctacggcg ttaggaaagg catctccgct
      241 cttgtacggg tcaccactag cttcgccgca agccgccagg atgcgcgccg cgcgggcgtt
      301 ggtctgcctg tcgatgcccc tgaccgctat ggtggagcaa tcgaagggcc gcgtctgccc
      361 tccccactgc acctgcacga tgcggctggt atcgctctgc tgcgtatcgg ctccaacctc
      421 gctagtgagg ccctgcggcc cctgccgcgt gggcagcccc gcctgggcca cgcccacaca
      481 cagggccagg gcaagcagta cgactgctgt gtgagtaaga aggcggcgta gagttaggtt
      541 cgcgggcatt tagcctcctc ttctgaagtt gcgatttatg gggtaccgag cggccctctg
      601 tcattctgag tgtagcgaag aatctaaaga agctggtaca gggaggctgt tgaaccatac
      661 gcactttggt gatgacgttc aacatagagg caaaactagc atcttagatt cttcgctacg
      721 ctcagaatga caggggccac ctttgttcgc ttgcgcccta cagtttgcag atactctcta
      781 gtatagcagc cataggtata atgctcatag aatgttcctt gctttctaca aggattgggg
      841 aacacgaaag ctcccaggcc gattttctag ctttacgata agcacatcct gggccggagg
      901 gatttgtggt ggacttgagc gaggtcttga ggcacaaggg cgagccgcgc accgaccact
      961 atgttgaggt acccaccccg ctgggcaaac tccggctgga cctgttgggg gaggtggggg
     1021 ccgcgctggc gcaggcaggc atgacgccgg aggcgcagca agccacggct aaggctatcg
     1081 acgcggcgag ggcaaagctt gcgacgggtg acggcgaagg ggcaaagcgg ttgctgctgg
     1141 acgcggcgaa gcagtaccct tataccgcga acgtgtacgg tatgctctac gacgttttca
     1201 ccgccgaagg cgaccttcca gaggccgagt tctgcatgaa gcaggctatt gccctcggcc
     1261 ccgacttccg caacctgacc ttcctggcgc gcaacctggg gcggcagggc aggttggaag
     1321 aagccgccac tatccaggag ttcctctggc agacccgctc ggaggccgca ccggagcagg
     1381 cgctggacgc tatacacgac tacctggtga cgctgggccg cctccaacaa ccgcaggcga
     1441 tgatggacgt gagcatgagg gccatgcagg agcacggcag cgagaccacc ctggtctacc
     1501 agtacgtatt cgccctcgtc ctctccaacc agcaagaggc ggccagggag cacctgcgac
     1561 gggtcctgcc ccagctcgcc gcggacgacc ccctgtaccc gcgcttcgtg caaatgagcg
     1621 actacctcgg cgggcggctg acgtagcggc ggctggctag gggttgggct gcacgcgctc
     1681 cacgtagtaa tgggtgccgc cggggcacat gacccgcgcg tcgcccggcc ccagcaagcg
     1741 gccaaacgcc accaccatgt ccccgtcctt gtattggctc tcgtcccagc ccggtccgcc
     1801 cggtataaag acgtcaccga ccggctggtt cgcctcggca gtgccgctgg cttggggcgt
     1861 gatgtcgcgt agggg
//
LOCUS       DASRWZ010000353         4445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_578848, whole genome shotgun sequence.
ACCESSION   DASRWZ010000353 DASRWZ010000000
VERSION     DASRWZ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578848"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            551..2644
                     /gene="fusA"
                     /locus_tag="VFH60_05455"
     CDS             551..2644
                     /gene="fusA"
                     /locus_tag="VFH60_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6313262.1"
                     /translation="MKLYNTAQLRNIGLFSHGGAGKTSLLEAMLFTSKGTNRLGSVAE
                     GNTVSDYDPDEIKRRISVQVSLAPVEWRDTKINVLDTPGYAEFVGEVKAAIRVVDTTV
                     LLVDAAAGVEVGTEQMWRYAEERGCPRFVVVNKMDRENADFMSAWHSTQNVLGKKCAP
                     LQIPIGSQHGFRGVVDLLHQKAYVYEGGNSGKFTEGPIPADLQADVQQLRDALVERVC
                     ETDDDLTMKYLEGEELSVEELDAALRAGVRAGDIVPVLAASGLLNVGVSQLLDTIVTY
                     APSPTDMGVMTATNAANGQQVELQPNDASPLTALVFKTIRDQFGKQTYFRVFTGSFRS
                     DSHVLNSSRNVEERIGQVYVMRGKEQMAVQELHAGDMGVVVKLAETQTGDTLCSVGTP
                     LIVDGINFPAALFSASIAPRTKADLDKMGTAVHHMVEEDPTIHVGKDPITGQTVLSAM
                     GESHVQIVADRMKRKFGVDVSVGLPVVPYRETITSRVDRAHYRHKKQTGGHGQFADVV
                     LELEPLPDAEFEFGDRIVGGAVPKNFIPAVEKGIREALSEGVLAGYPVVNIRAVLYDG
                     SFHPVDSSEMAFKLASNQAFKEGMQSGRPVLLEPIMLLHIIVPDNYTGDIMSDLNGRR
                     GRVLGMNPQGNGFTVIDAQAPLSEVQRYVSDLRSITQGRGSFDMEFDHYDPVPAHVAD
                     RVIEEARKHREEAHA"
     gene            complement(2851..4302)
                     /locus_tag="VFH60_05460"
     CDS             complement(2851..4302)
                     /locus_tag="VFH60_05460"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET6313263.1"
                     /translation="MSGVATQPTPSPSPTYDRTPVSVPDPRLALLRSEPVILVAMLLA
                     LALYYFVPTLPVRGIGLAAFFALTLYRLDLSLAMVPLAAPLLYHYYPLGRLQFSLAEA
                     IILCQVGAWAVRDGLTLLRTRKLTAIGNMLRQPLVWLALLFGAIATLWLLVPGGENRQ
                     FAIREYRVTILEPLLFFMLVVRWLRTERDVWRMVAAWLIASALVGYVGVEQFLFGEAW
                     NMEGVNRVSSVYPSATAFGIYAGRALALGLVLVYFLPREWRRWRIAVGLLSGIIGLGV
                     LFSFARGAWIGVFAGVAVVALVTRYRPLLISVGGVVAAGVAAILLAFAGNVERLTSIF
                     NLATEDNTGVARFRIWEAGRKILADHPLLGIGQDQFAYQDPAYGVPVARLFTTSHPHN
                     FLLDFWLRLGVPGLLWILGTLALVTWQFQVLWLRHKGTALGALSLGLLASMIDFAVHG
                     LLDMAYFTMDLALTFWLTLALLVLMKKLGPTAV"
BASE COUNT          918 a         1378 c         1432 g          717 t
ORIGIN      
        1 ctaccctccc accaagtaca acaccagaat gcagacctac gcgagtgcca agacgtgaat
       61 gcactaagct gctttagcgg gaccatctcc cgcctttgtc attctgagcg cagcgaagaa
      121 tctcgtcctc cagcaccaaa atagcccttt tctttacgcc ggaaaggtct atttgccgcc
      181 gtgtgctacg gaaaaaggca tcatcgatgt tgaggacgag attcttggct acgctcagaa
      241 tgacaagaac cacccttgtt cccttgcttc tacactttgc agatagaccc tacccttatg
      301 ttccttagtc cccgcagggg actttgcctc ttctcagccc gtggctttag ccacagggcg
      361 aaggtggcag ggcatctccc ggattaacct cttcgctgct gagaacggct atacaaggtg
      421 tagccctggc aaccaaatca cgttttaagt ataatcacag gacagtcgcc gggtaagtta
      481 gcctgggcgc tttgccgtac ccagtcgagg gtaagatgac tagagagtgc aggagggtct
      541 atctggcttt atgaaactct acaataccgc acaacttcga aacatcggcc ttttttcgca
      601 tggtggggca gggaagacgt ctttgctgga ggcgatgctc tttacctcca aaggcaccaa
      661 ccgcctgggc agcgtggccg agggtaacac cgtttccgac tacgaccccg acgagatcaa
      721 gcgccgcatc tcggtgcagg tctccctcgc gcccgtcgag tggcgggaca ccaagatcaa
      781 cgtgctcgac acgccgggct acgccgagtt cgtgggcgaa gtgaaggccg cgataagggt
      841 ggtggacacc accgtgctgc tcgtggacgc agccgcgggc gtggaggtgg gcaccgagca
      901 gatgtggcgc tacgccgagg agcggggctg ccccaggttc gtggtcgtga ataagatgga
      961 ccgcgagaac gccgacttca tgtccgcctg gcattccact cagaacgtgc tcggcaagaa
     1021 gtgcgcgccc ttgcagatac ccatcggctc gcagcacggc ttccggggcg tggtggacct
     1081 gctgcaccag aaggcatacg tctatgaagg gggcaacagc ggtaagttca ccgaaggccc
     1141 catccccgcc gatttgcagg ccgatgtgca gcaactccgc gacgccctgg tagagcgcgt
     1201 gtgcgagacg gacgacgacc tgacgatgaa gtacctcgaa ggcgaagagc tttcggtgga
     1261 agagctggat gcggcgctca gggcaggggt cagggcaggc gacattgtgc ccgtgctggc
     1321 cgcttcggga ctgctgaacg tcggcgttag ccagctactc gatacgattg tcacctatgc
     1381 accctcgcct accgatatgg gcgtgatgac cgccaccaac gcggccaatg gacagcaggt
     1441 cgagctgcag ccgaacgacg cctctcccct cacggccctc gtcttcaaga cgatacgcga
     1501 ccagttcggc aagcagactt acttccgcgt gttcaccggc agcttccgct ccgacagcca
     1561 cgtgctcaac tcgtcccgca acgtggagga gcgcatcggc caggtgtacg tgatgcgcgg
     1621 caaggaacaa atggcggtgc aggagctgca cgcgggcgat atgggcgtgg tggtcaagct
     1681 ggccgaaacg cagaccggcg acacgctctg ctccgtgggc accccgctca tcgtggacgg
     1741 catcaacttc cccgccgccc ttttctcggc atccatcgca ccgcgcacga aggccgacct
     1801 cgacaagatg ggcaccgccg tgcaccacat ggtagaggaa gaccccacga tacacgtcgg
     1861 caaggacccc atcacggggc agacggtgct ttccgcgatg ggcgagagtc acgtgcagat
     1921 agtggccgac cggatgaagc gcaagtttgg cgtggacgta tcggttggcc ttcccgtggt
     1981 gccctaccgc gagacgataa cgtccagggt agaccgcgcg cactaccggc acaagaagca
     2041 gaccggcggg cacgggcagt tcgccgacgt ggtgctggag cttgagccgc tgcccgacgc
     2101 cgagttcgag tttggcgacc gcatagtggg cggcgcggtg cccaagaact tcatcccggc
     2161 ggtggaaaag ggcatccgtg aggcgctgtc cgagggcgtg ctggcgggct acccggtggt
     2221 caacatccgg gcggtgctct acgacggcag cttccacccg gtagatagct ccgaaatggc
     2281 attcaagctg gcgtccaacc aggcgttcaa ggagggcatg cagagcggcc gcccggtgct
     2341 gcttgagcct atcatgctcc tgcacatcat cgtccccgac aactacacgg gcgacatcat
     2401 gtcggacctg aacgggcggc gtggtagggt gctgggcatg aacccgcagg gcaacggctt
     2461 tacggtaatc gacgcgcagg cccccctctc ggaggtgcag cgctacgtca gcgacctgcg
     2521 ctccatcacc cagggccggg gcagcttcga catggagttc gaccactacg accccgtgcc
     2581 cgcgcacgta gccgaccgcg taatcgaaga agcccgcaag caccgcgaag aagcccacgc
     2641 ctagccccac gagccagctt tgtggccggt ggtagcaatt aacgctgaaa ccgcacaggc
     2701 gcaaagagcg caaaggacac agaacgatat agtaggtgtt ctaagcaaga ggaccagggt
     2761 acacgattag taccctggtc ctttttgtac tcgctaaacc atccttaaac tttgcgctct
     2821 ttgcgccttt gcggtttcat cgtctttatt ctacaccgcc gtgggaccaa gtttcttcat
     2881 gaggacgagg agggcgaggg tgagccagaa ggtgagggcg aggtccattg tgaagtaggc
     2941 catgtcgagg aggccgtgaa cggcgaagtc gatcatggag gcgaggaggc cgagggagag
     3001 cgcgccgagg gcggtgcctt tgtgacggag ccacaggacc tggaactgcc aggtgaccag
     3061 ggcgagggtg ccaagtatcc aaaggaggcc cggcacgccg aggcgcagcc agaagtcgag
     3121 caggaagttg tgagggtggg aggtcgtgaa gaggcgggcc accggcactc catacgcggg
     3181 gtcctggtag gcgaactggt cttggccgat gcccaagagc gggtggtcgg ccagtatctt
     3241 gcggccggcc tcccagatgc ggaaccgcgc tacccccgtg ttgtcctccg tagcgaggtt
     3301 gaagatggag gtgagccgct ctacgttgcc tgcgaaagcc agcaagatgg cggccacacc
     3361 cgccgccacg accccaccca ccgaaatgag cagcggacgg tagcgcgtga ccagcgccac
     3421 cacggccaca cccgcgaaca cgccaatcca ggccccgcgg gcgaaactga acaggacccc
     3481 caggccgatg atgcccgaca gcagccccac cgctatgcgc cagcgcctcc actcgcgggg
     3541 caggaagtag acgagcacca gccccaacgc aagcgcccgc cccgcgtaga tgccgaaagc
     3601 cgtggcgctt gggtagaccg aggagacgcg gttgaccccc tccatgttcc aggcttcgcc
     3661 gaagaggaac tgctccacgc ccacgtagcc taccagcgcg gacgcaatca gccaggcggc
     3721 caccatgcgc cagacgtccc gctcggtgcg cagccaccgc acgaccagca tgaagaacag
     3781 cagcggctca aggatggtca cccggtactc gcgtatcgcg aactggcggt tctccccgcc
     3841 cggcaccagc agccagagcg tggcgatcgc gccgaatagc agggccagcc acaccaacgg
     3901 ctgtcgcaac atgttgccga ttgcggtcag cttgcgcgtg cggagtaatg tcaggccgtc
     3961 gcgcacggcc cacgcgccca cctggcacag gataatggcc tcggcaagcg agaattgcag
     4021 cctgcccagc gggtaatagt ggtagaggag cggggcggcc aggggcacca tcgccaggct
     4081 caggtcgagg cggtagaggg tgagggcgaa gaaggcggca aggccgatac ctcgcaccgg
     4141 gagcgtgggc acgaagtagt agagggccag ggccagcagc atggctacca ggattaccgg
     4201 ctctgagcgc aggagcgcca gccgcgggtc gggaacgctg acgggcgtgc ggtcgtaggt
     4261 ggggctgggg gagggcgtgg gctgcgtggc tactccgctc aagcgtccgc ccccgttccg
     4321 accggcttct tgcggggcat cagcaggagc gccagaccgc tcgcgagcac cagcccgacc
     4381 accacgttcg ccccgtaggc caacatgcgg cgggtggaag agatactgtt cgggccgtgg
     4441 gcgtc
//
LOCUS       DASRWZ010000354         1690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_235321, whole genome shotgun sequence.
ACCESSION   DASRWZ010000354 DASRWZ010000000
VERSION     DASRWZ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235321"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1266
                     /locus_tag="VFH60_05465"
     CDS             178..1266
                     /locus_tag="VFH60_05465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6313264.1"
                     /translation="MQVDWQVRAYRPGDERQLVSLYAEVFGRPRSIEAWRWKLLGRDG
                     SPENVWVAVTSEGRIVGQYGGIPLHIKLRGEIHPAVHAVEAMTSPDYRRQGMLTRLGS
                     AAHDAWAKSGRVMVIGLPNEQWGTRNRALGYTDLFPLGWLRVPLHMERVAARPGKLPR
                     PLARPASVLGGAGSRAWRSLSRAQMRRKTAGAGLSVDEITSPSADFDRLWASLSPMYD
                     NCVVRDSSWVGWRFIAAEGYHYRVLLARSEGEPVGYIAYRLAESAERTTGYIDDIFVG
                     PDGQAVTAKLLEAALDDLSARGAGTVMATAAPGSALYGHLRSMGFLPGRASTAFMFEI
                     VPLQPGVEPGTLADPRTWHITAGDFDVV"
     gene            1281..>1690
                     /locus_tag="VFH60_05470"
     CDS             1281..>1690
                     /locus_tag="VFH60_05470"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017871724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET6313265.1"
                     /translation="MDARWVLKWLAHHALFPLVEGIYAAGFALAGRLLRPGVRLSTPN
                     GNDSVLVIAPHPDDETLGCGGTLALHSRAGGQVCVLIVTDGGSSRAGGLGREEMRRRR
                     HDEAASAMSALAPVELIQLGLPEGGWPPEELYASL"
BASE COUNT          276 a          526 c          587 g          301 t
ORIGIN      
        1 gtagctttcc aaacgagcgc gccattcctg gacccaatac tgacccctaa tcccctaacc
       61 cctgatccct gatccctcgt ccgcaccgct tgtcagcgtc caggcaggaa gttatactga
      121 gggcacttat gtggcctggg atgaggggtt atcgccttgc gtagtttaga gcatcgggtg
      181 caggttgatt ggcaagtgcg cgcttaccgg cctggggacg agcgccagtt ggtcagcctc
      241 tacgccgagg ttttcgggcg gccccgctcg atagaggcat ggcggtggaa gctcctgggg
      301 cgcgacggct cgcctgagaa cgtgtgggtg gccgtgacta gcgaaggccg gatcgtaggg
      361 cagtatggag gtatccccct gcacatcaag ttgcgtgggg agatacaccc ggcggtccac
      421 gctgttgaag cgatgacttc ccccgactac aggcggcagg ggatgctcac tcgcctcggc
      481 tctgccgcgc acgacgcatg ggcgaaatca ggccgcgtga tggtgattgg gctgcccaac
      541 gagcagtggg gcacccgcaa ccgcgccctg ggttacacag acctcttccc gctcggctgg
      601 ctccgcgtcc ccctccacat ggagcgcgtg gcggcccgcc ccggcaagct gccccgaccc
      661 cttgcccgcc cggcatccgt gctggggggc gcaggctcgc gggcatggcg cagcctgtcg
      721 cgcgcccaga tgcgccgaaa gacggcggga gccggtttga gcgtggatga aataacctct
      781 ccgtcggcag atttcgaccg cctgtgggca tctctgtcgc ccatgtacga caactgtgtg
      841 gtgcgcgata gctcgtgggt ggggtggcgg ttcatagcag cggagggcta ccattacagg
      901 gtcctgctcg cccgctcaga aggcgagccg gtgggttata tcgcgtaccg gctggccgag
      961 tctgccgagc gtaccaccgg ctacattgac gatatattcg tggggccgga cgggcaggca
     1021 gtgacggcaa aactgcttga ggcggcgctg gacgacctct ctgccagggg cgcgggcacg
     1081 gtgatggcga cggcagcgcc cggctctgcc ttgtatggtc atctacgctc gatgggcttc
     1141 ttgccgggaa gggcttccac agccttcatg tttgagatag tgcctctaca gccgggcgtc
     1201 gagccgggca cgcttgccga cccgcgcacc tggcacatca ccgcgggcga cttcgacgta
     1261 gtatgatggc taagagaagc ttggacgctc gctgggtgct gaagtggttg gcgcaccacg
     1321 cgctcttccc cctggtggaa ggcatctacg cggcagggtt cgcgctggcc ggcaggctcc
     1381 tccggccagg ggtgcggcta tctacgccaa acggcaacga cagtgtgctg gtgatagcgc
     1441 cccaccccga cgatgaaacg ctgggctgcg gcggcaccct ggccctacat agcagagcgg
     1501 gcggccaggt gtgcgtgctc atcgtcaccg acggaggtag ctcaagagcg ggcggcctgg
     1561 gccgcgagga gatgcggcgg cggcggcacg atgaggcggc aagcgccatg tccgcgctcg
     1621 cgcccgtgga gcttatacag ttgggcctcc ccgaaggagg gtggccgccg gaggagttat
     1681 acgcctcgct
//
LOCUS       DASRWZ010000355         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_98787,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000355 DASRWZ010000000
VERSION     DASRWZ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98787"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..818
                     /locus_tag="VFH60_05475"
     CDS             33..818
                     /locus_tag="VFH60_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6313266.1"
                     /translation="MSAQQPEQSARSVAIVTGASRSRGIGAAVCVELARAGMDIFFTH
                     WTPYDIAMEFGDEEDTPAALKSRLVSLGVRCEHMSADLADVETPKRIMDTVEERLGVA
                     SVLVNNAVHDTTDAPFHELDAQTLDAYYAVNIRGTMLLSVEFARRFTRGAGGRIINLT
                     SGQGRGPMPGKLAYVATKGAIEAFTVTLSAEVAPAGITVNAVDPGPTDTGWVTDELRE
                     YLLPRFPMGRVGQPEDAARLIAFLASENAAWVTGQIIRSTGGF"
     gene            complement(917..2245)
                     /locus_tag="VFH60_05480"
     CDS             complement(917..2245)
                     /locus_tag="VFH60_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6313267.1"
                     /translation="MITRRALFLLLIAAPILALATITSAALWAAGLYLLGVLFFLALD
                     FLLSPRPGQFRLERRNESKLSLGADNLITIVLRRLGNPEDRLRGRPVTFLVRDEPPTE
                     YIISRMFMPGAIVPGETVELEYTARPLRRGDYRFGGCYLRYSGVLGLVIRQHRYPLEA
                     PVKVYPNLLEVRKYELLARKDQLFEIGLKNARRLGQGTEFERLRDYEQDDDYRRINWP
                     ATARLHKPIMTDYQPERSQSVIVMLDAGRLMTAPLGSLSKLDYAINTTLLLAYVAIVR
                     GDRVGLLTFADTVTGYWEPDKGRRQFLTLLEALYKVQAQQVEPDYMRAGAFLSTRRSK
                     RSLVVLFTDILDTQQAGSMVRGFGRLAPRHLPLAVTMSDTEVLAMSRRIPGDTQQMYE
                     KVLAQNLLDDRATALEALQRLGALTLDVPADKLTISVVNKYLELKARGRL"
     gene            complement(2232..2717)
                     /locus_tag="VFH60_05485"
     CDS             complement(2232..2717)
                     /locus_tag="VFH60_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6313268.1"
                     /translation="MSYDIRYATSEDADVVHRIIQAAFAEYDGVIAVPPGALAETVEQ
                     AAQAIGEGTALLAYDGEEAVGTVRYEVRPDYVYVGRLAVLPTHRRRSVGAALMAYVES
                     LAPALGRSKVHLWTRLSMPSNLAFYERLGYRIVNTEPHPRGNDTNVWFEKELGPPVDN
                     T"
BASE COUNT          527 a          851 c          997 g          513 t
ORIGIN      
        1 cgggggctgg aggactgaag gagtaagcac cattgtcagc ccaacaacct gagcaatccg
       61 ccaggtccgt cgccatcgtc acgggggcga gtcgaagcag gggtattggc gcggcagtat
      121 gcgtggagct tgcgcgcgcg ggtatggaca tctttttcac gcattggacg ccttatgata
      181 tagctatgga gtttggggac gaagaagata cgcctgccgc tctaaagtcg cggctggtat
      241 cgcttggggt gcgctgcgag catatgtctg ccgacctggc tgacgtggag accccgaagc
      301 gcatcatgga tacggtcgag gagcgcctgg gcgtggcatc ggtgctggtc aataacgctg
      361 tgcacgacac cacagacgcc ccgttccacg aactcgacgc gcagacgctg gatgcctact
      421 acgccgtgaa catcaggggc acaatgctgt tgagcgtgga gtttgcgcgc cgctttacga
      481 ggggagccgg tgggagaata atcaacctga catccggaca ggggcggggt ccgatgccgg
      541 ggaagctggc gtacgtggct accaaggggg caatcgaagc gtttacggtg acgctctccg
      601 cggaggtagc gccagcaggc ataacggtga atgccgtgga ccccggcccc accgacaccg
      661 gctgggtgac ggacgagctg cgcgagtacc tgctgccacg gttcccgatg ggcagggttg
      721 gccagcccga agacgcggca cgcctgatcg cattcctggc gagcgagaac gccgcctggg
      781 tgacgggcca gattatccgc tctacaggcg gcttctgaca ctaaaactct ttgagtggac
      841 tattaagaag aaaggggaag agagttgggg acacgcccaa actcctgtca agggagtacc
      901 ctggtacccc agatgatcat agtctgccgc gtgcctttag ctcaaggtac ttgtttacca
      961 ccgagatggt gagtttgtcc gcgggcacgt cgagggtgag cgcgccgagg cgttggaggg
     1021 cttcgagggc ggtggcccgg tcgtcaagca ggttctgggc gagcaccttc tcgtacatct
     1081 gctgggtgtc gccggggatg cggcgggaca tcgcgagcac ctcggtgtcg gacatggtga
     1141 cggcgagtgg caggtggcgc ggggccaggc ggccgaagcc tcgcaccatc gaacccgcct
     1201 gctgggtgtc cagtatgtca gtaaagagca ccacgaggct gcgcttgctg cggcgggtcg
     1261 agaggaaggc tccggcccgc atgtagtcgg gttcgacctg ctgggcctgc accttgtaga
     1321 gcgcctcaag cagcgtgaga aactgccgcc gccccttgtc aggctcccag tagcccgtga
     1381 ccgtgtcggc gaaggtgagc aggcccacgc ggtcgccccg cacgatagcg acgtaggcca
     1441 gcagcagagt ggtattgatg gcatagtcca gcttggagag cgatccgagg ggggcggtca
     1501 tcagtcggcc cgcgtctagc atgacaatca cgctctggct gcgctccggc tggtagtcgg
     1561 tcatgatggg cttgtgcagg cgggcggtgg cgggccagtt gatgcggcgg taatcgtcgt
     1621 cctgctcgta gtcgcgcagc cgctcgaact cggtgccctg gcccaggcgg cgggcgttct
     1681 tcaggccgat ctcgaatagc tggtccttgc gcgccagcag ctcgtacttg cgcacttcca
     1741 gcaggttggg gtagaccttc acgggggctt cgagcgggta gcggtgctgg cgaatgacaa
     1801 ggccgagcac gccgctgtag cgcaggtagc aaccgccaaa cctgtaatcg ccccgccgta
     1861 ggggcctggc ggtgtactcc agctccaccg tttcgcctgg gacaatcgcg cccggcatga
     1921 acatccggct gatgatgtac tcggtgggcg gctcgtcgcg cacgaggaag gtgacggggc
     1981 ggcctcgcag gcggtcctca gggttgccga gccgccggag cactatcgtg atgaggttgt
     2041 ccgcgccgag ggagagtttc gactcgttgc ggcgctccaa cctgaactgg cccggcctgg
     2101 gtgacagcaa gaaatcgagg gccaggaaga acagcacgcc cagcaggtaa agccccgcgg
     2161 cccacagggc ggcagaggta atagtagcca gcgcgaggat gggcgcggcg atgaggagga
     2221 ggaataacgc tctacgtgtt atcaacgggc ggccctagct ctttctcaaa ccagacgtta
     2281 gtgtcgttgc ccctggggtg aggctcggtg ttgacgatgc ggtagcccag ccgctcgtag
     2341 aaggcgaggt tgctgggcat cgagaggcgg gtccacaggt gtaccttcga gcggcccagc
     2401 gcaggtgcca ggctctcgac ataggccatg agggcggcac ccacgctgcg ccgccggtga
     2461 gtgggcagca ccgccagccg ccccacgtag acgtagtcag gtcgcacctc gtagcggacg
     2521 gtgcccaccg cctcctcacc atcgtaggcc agcagcgccg tgccctcccc tatcgcctgc
     2581 gccgcctgtt cgaccgtctc ggccagggca ccgggaggca cggctatcac gccgtcgtac
     2641 tcggcaaagg ccgcctggat gatgcggtgc accacgtcgg catcttcgga cgtggcgtac
     2701 cgtatgtcgt agctcatggt ggtattcagc ggagcgttcc cttatttcag cattacagca
     2761 ctcgaagtcg cggcgaccaa gagtgccccc tgtcatcctg agcgtagcga aggatctaag
     2821 atgcggctat tacctctgcg ttggaccccg ccgcgatgat tcctgacgtt caacggcatg
     2881 cctctcca
//
LOCUS       DASRWZ010000356         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_853006, whole genome shotgun sequence.
ACCESSION   DASRWZ010000356 DASRWZ010000000
VERSION     DASRWZ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853006"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFH60_05490"
     CDS             complement(<1..422)
                     /locus_tag="VFH60_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HET6313269.1"
                     /translation="MSLPDLRSQATRRTPEELAAHLNSFELDLKFSAGVWFFSPPASR
                     FHDRYNPVIEIEPRLEIAARLADQGLRGMEAHYPNEINESNLETWRQFTRDTGIKLIT
                     VIPLLFYDAQFEWGSLSNPLPEIRRKAIDRTIAALELNR"
     gene            complement(788..1252)
                     /locus_tag="VFH60_05495"
     CDS             complement(788..1252)
                     /locus_tag="VFH60_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313270.1"
                     /translation="MTRTDDNTGGKEGGSKELRPRLPIRRFDVFAEYNRQKAIEDGTP
                     PDEAMGYGLWVAKVVASRSFGRSVLSKPPSELRKSDEGDDADEGDAPPDKPKWHSLEG
                     KPQTDELFEKEIVRRMGADFYIEVFVPAIEQAIAQDRSYTSIRDTIRKEWKP"
     gene            complement(1265..1483)
                     /locus_tag="VFH60_05500"
     CDS             complement(1265..1483)
                     /locus_tag="VFH60_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313271.1"
                     /translation="MSQDQDNEQQGENEKTHQTFSGNPHKSEAVGDEGGSQITGNPGH
                     AGAGGAGGQGGGSGGGGGQGDFKQGHGG"
     gene            1688..3157
                     /locus_tag="VFH60_05505"
     CDS             1688..3157
                     /locus_tag="VFH60_05505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6313272.1"
                     /translation="MRRPWRSQRRDWVAAFLLLCALAPYAPNLDAFFLSDDFVLLSWT
                     HVRSPSEVPGFFDPNTEWFYRPVVKLVYWAGQSLFGFRAAPFHLLSLLLHLANAYLLY
                     RLVSRSRLGWTAGLVAALVFMLDPHHAETVSWVSAVGDLIGTLCILANLLLLRGYLRG
                     GRLIYVAPALALFAAGLLARETVLLLPGLALLYLLLLETRSVLSRARRGLVLWLGGYV
                     VIAAGYLALLRLGSGGAGGGLARGGLEFRPLNPDSVLLGLLDYAHRLVPGGEMLANAP
                     LDALRVLVWGEWAALLLLGLALWRLRLTYVLFGLAWLLLAPAAFIFFSPPTDRYFYLP
                     SVGFAIFLGCLLGSLLALVARRAGAWRVGARALACLFLVGLLLTHGTTLTSRVAEWRA
                     AGHASGGIVNDLRRTVPNPEDYAAYFFVDLPVFLEGVPIFQNGLQQAVQLTYDNPTIA
                     AQAATCTQLQAQPEFPRYSYFFRYKPNGITQLASPQDCR"
     gene            complement(3403..>3768)
                     /locus_tag="VFH60_05510"
     CDS             complement(3403..>3768)
                     /locus_tag="VFH60_05510"
                     /inference="COORDINATES: protein motif:HMM:NF025643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HET6313273.1"
                     /translation="VYLQDPRSILVLVWLDPAQPGKAKLSLLQIGEGAGLAEKMVTRP
                     EVIEETTVNGKKAAWVRGPHTLVFVVANQRTEFQEHMLVEGNVLLWQDNIVTYRLETD
                     LPKEEAIKIAESLVEVPLR"
BASE COUNT          577 a         1241 c         1104 g          846 t
ORIGIN      
        1 cggttcaact ccagcgcggc aatcgtgcgg tctatggcct tgcgcctgat ttcggggagc
       61 gggttggaga gcgaacccca ctcgaactgc gcatcgtaga agagcagcgg tatcaccgtg
      121 atgagcttga tgcccgtgtc gcgggtgaac tgccgccagg tctcgaggtt gctttcgttg
      181 atctcgttgg ggtagtgcgc ctccatgccc cgcaagccct ggtcggcaag gcgtgcggcg
      241 atctccaggc gcggctctat ctcgatcacg gggttgtagc ggtcgtggaa gcggcttgcg
      301 ggcggcgaga agaaccagac ccccgccgag aacttcaagt caagctcgaa cgaattcaga
      361 tgcgcggcca actcttccgg cgtccggcgc gtggcttgcg aacgcaggtc cggcagactc
      421 ataacggtct tcctcctcag tctgattagt agaggttctg aaaggccaca cgtgaaacct
      481 tacgggtttg ctgagcacag gggaggtgaa tgccactctg tttctattgc gaggttctgt
      541 tggtccctgt cattctgaac ccttcgctac gctcagggta aactccgtga agaatctcgt
      601 ccttcactaa agtgttgcct ttttcttctg cgagatagta gcacaaggaa aggggcagct
      661 tgggtgctaa aggacgagat tcttcgctac gctcagaatg acaaaagggg agagggttcc
      721 gtaacagaat catccccacg cttacggtcc aattataatg cgccgtagca cccttcagaa
      781 accaatctca aggcttccat tccttgcgta tagtgtcgcg aatcgaggtg tagctgcggt
      841 cctgcgctat ggcctgctcg attgccggga cgaacacctc gatgtagaag tctgccccca
      901 tgcggcgcac gatctccttc tcgaaaagct cgtctgtttg cggcttgccc tccagggaat
      961 gccacttggg cttgtccggc ggggcgtcac cctcgtcggc atcgtcgccc tcatccgact
     1021 tgcgaagctc agagggtggc ttcgagagca ccgaacgtcc aaagctgcgc gaagccacta
     1081 ccttcgccac ccacaggccg tagcccatcg cctcatcggg tggagtgccg tcctcgattg
     1141 ccttctgccg gttgtactcg gcaaacacat cgaaccggcg gatgggtagg cgcggcctta
     1201 gttccttgct gccgccttcc ttcccgccgg tgttgtcatc ggtccgggtc atgttgttac
     1261 cctgtcagcc gccgtggccc tgcttgaagt caccctggcc gccacctccg ccgctgccgc
     1321 caccctggcc gcccgcaccg cccgcgcccg cgtggcctgg gttgcccgtt atctggctgc
     1381 cgccttcatc gcccacggcc tcgctcttgt gcgggttgcc actgaaggtc tggtgggtct
     1441 tttcgttctc gccctgttgc tcgttgtcct ggtcttgaga catcagcgat tctcctcttg
     1501 gacttgggac ctcaactcac ggctgcgccc tcaggtggga cgctgcaagt gatgtgccag
     1561 gacacgggac acggcgcggc atgagctata atggcctgta tgagcgagcc aacgcccagc
     1621 tacccaggcc tgacttccgg ctacaaagcc ggcctcccct cagcgtggat accgcgcccg
     1681 gtgagccatg cgtaggccct ggcggtcaca gcggcgcgat tgggtcgccg ccttcctcct
     1741 gctctgcgcg ctcgcacctt acgcgcccaa cctggatgcc ttcttcctct ccgacgattt
     1801 tgtgctgctc tcctggaccc acgtccgctc tcccagcgaa gtgccgggct tcttcgatcc
     1861 caataccgag tggttctacc gcccggtcgt caagttggtc tactgggcag gacaaagcct
     1921 gttcgggttc cgcgccgccc cgttccacct gttgtcgctg ctgttgcacc tggcaaacgc
     1981 ctacctgcta taccgccttg tatcgcgctc tcggctgggg tggacggcgg ggcttgtggc
     2041 agcgctggta ttcatgctcg acccacacca tgccgagacc gtatcgtggg tgtcggcggt
     2101 gggcgacctg attggcaccc tctgcatact cgccaacttg ctcctgctac gcggctacct
     2161 cagaggcggc aggctcattt acgtagcccc ggccctggcc ctgttcgccg ccggtctgct
     2221 ggcgcgggag acggtgctgc tcctgcccgg cctggctttg ctgtacctgc tgcttctcga
     2281 gaccaggtcg gtgctatcgc gggcgcggcg gggcctggtg ttgtggctgg ggggatatgt
     2341 cgtcattgcg gcggggtatc tggcgctgct acgcctgggg agtggtggcg cagggggagg
     2401 gttggctcgt ggagggctgg agttccggcc gctgaaccca gactcggtcc tgctcggctt
     2461 gctggactac gcccacaggc tcgtgccggg cggggagatg ctggcgaacg ccccgctcga
     2521 cgctctccgg gtgctggtct ggggcgagtg ggcggccctt ctcttgctgg gcctcgcgct
     2581 ctggcggctg cgcctcacct acgtgctgtt cggcctggcg tggctcctac tcgcccctgc
     2641 cgccttcatc ttcttcagcc cgcccacgga caggtacttc tatctcccca gcgtgggttt
     2701 cgccatcttc ttgggctgtc tgctggggag tctgctcgcc ctggtcgccc gccgtgcggg
     2761 agcctggcgt gtgggtgcca gggcgctcgc ctgcctcttt ctggtgggcc tacttctcac
     2821 gcacggcacg acgcttacct cgcgggtcgc ggagtggcga gcggcgggcc acgcttcggg
     2881 cggcatcgtc aacgacctgc gccgcaccgt gcccaacccg gaggactatg cggcctactt
     2941 cttcgtggac ttgcctgtct tcctcgaagg cgtgcccatc ttccagaacg gcctgcaaca
     3001 ggcggtgcag ttgacctatg acaatcccac aatcgccgcc caggccgcca cctgcaccca
     3061 gttgcaggcc caacctgagt tcccccgcta cagctacttc ttccgctaca aacccaacgg
     3121 catcactcaa ctcgcctccc cacaagactg ccgctaacct gtcctccaca gttgaaacgc
     3181 cgcgacgcca agacgccaag aaccacgaca agattaagta ggtgttctaa gcaaaaggag
     3241 cagggtacga cctcatgtat cctgctccct cttgtactct acaaacaatc ctatctttgc
     3301 gcgttctttg cgttctttgc gccttgcgtt ttcgtattct tggcgtggtc cttggcgttc
     3361 ttggcgtctt ggcggttccg tctccttcgc cacaacccga atctacctta gcggcacttc
     3421 caccagcgac tccgcgatct tgatggcttc ttccttgggc agatccgttt ccagcctgta
     3481 ggtcacgata ttgtcctgcc agagcaggac gtttccttca accagcatat gctcctggaa
     3541 ctcggtcctc tggttcgcta ccacaaagac gagtgtatga ggcccgcgca cccaggccgc
     3601 tttcttcccg ttcaccgtgg tctcctcaat cacttccggg cgagtaacca tcttctcggc
     3661 cagccccgcg ccttccccga tctggaggag gctcagcttg gccttgccgg gctgcgcagg
     3721 gtcgagccac accagcacca gtatcgagcg tgggtcttgc aggtagac
//
LOCUS       DASRWZ010000357         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_441637, whole genome shotgun sequence.
ACCESSION   DASRWZ010000357 DASRWZ010000000
VERSION     DASRWZ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441637"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1433)
                     /locus_tag="VFH60_05515"
     CDS             complement(51..1433)
                     /locus_tag="VFH60_05515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET6313274.1"
                     /translation="MRASQEPTDDPVELERRLLSQGPGARGQGPGARDRGRWTLVGKV
                     AVALTVGLVVSYLLMWAGTVQQFGGPEGYVRRTDFVSTLTGGLIIRDGNGPLLYDLEA
                     QRAAQNRVLAPYSQLDPGKVLPYNHIPFEAMLVSLLADLPYPLVFALWTLAAGLAIGL
                     ALGIMDGALPVTRSTGWVISLAACSYLPLIRSLMLGQNSPLVLMGLCATYVAIRWEQP
                     GWAGASLLLVALKPQVLPIVLLLLILQRQWKPILVFAILMAALSLLAAPVLGLEWPLH
                     YARLLLGVAGWSDPDAINPAIMHNWRGFSTNLFDGWLPGIVSPLYILLSLASVGLVVW
                     AWYRSRNAPESEPMQPQKPATDLIWALAGITAVLISPHLNPHDLTLLIFPAWIIGSYA
                     SSGAWSGSMSRMWFFILWAGYTVGPLILVDLQSAVLPGVLVMAIASVLLAWQISQRAA
                     HTATHLAQPV"
     gene            complement(1423..>2063)
                     /gene="purQ"
                     /locus_tag="VFH60_05520"
     CDS             complement(1423..>2063)
                     /gene="purQ"
                     /locus_tag="VFH60_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET6313275.1"
                     /translation="TATDVSRYALVLLPGGFSYGDYLRAGAIARFTPVIEAVRRHAQE
                     GGWVLGICNGFQVLTEAHLLPGALMRNSELAFSCRWVHVRVEPSGCALLEGVQPGTVW
                     RLPIAHGEGRYFASPQTLAELNESGQVVLRYSTPEGSLDPAANPNGSSDAIAGICNRE
                     GNVLGLMPHPERAAEAILGGEDGRLFLQAVIDAWQRRATPASANQVEGVLAR"
BASE COUNT          412 a          635 c          695 g          321 t
ORIGIN      
        1 gggaagggct gggggttggg gtgttggatt tcagaatcta caaacctgaa tcaaacaggt
       61 tgagcgaggt gagtagcggt gtgagccgca cgctggctta tctgccacgc cagcaggacc
      121 gaggctatag ccatgacgag cacgcccggc aggaccgcgc tctgcaggtc cacgagtata
      181 agcgggccga ccgtgtaacc cgcccacagg atgaagaacc acatgcgcga catgctgccg
      241 ctccatgcac ccgatgaggc atacgacccg attatccatg cggggaagat gagcaacgtc
      301 aggtcgtggg ggttgaggtg tggcgagatg agcacggctg tgatgcccgc cagcgcccat
      361 attaggtctg tggcgggctt ttggggctgc ataggctcgc tctccggcgc attgcgcgag
      421 cgataccatg cccacactac aaggcccacc gaggcgagag agagcaggat gtagagcggc
      481 gagacgatgc cgggcagcca gccgtcgaac aggttggtgg agaagccgcg ccagttgtgc
      541 atgatagctg ggttgatggc gtccgggtcg gaccagcccg ccacgcctag taggagcctg
      601 gcatagtgca agggccattc taagcccagc accggcgcgg ccaacaaaga tagcgcggcc
      661 atcagaatag cgaataccag gatgggcttc cactggcgct gtagtatgag caatagcagc
      721 acgatgggaa gcacctgcgg cttgagcgcg accagtagga gggaagcccc cgcccagccg
      781 ggctgctccc accttatagc tacgtaagtg gcgcacaacc ccatgagcac gaggggcgag
      841 ttctggccga gcatgaggga gcgtatgagg ggaagatatg agcaggcggc aagacttatc
      901 acccaacccg tagagcgggt gacgggcagc gcgccatcca ttatgccgag cgcgaggccg
      961 atagccagcc cggcggccag cgtccacagc gcaaagacca ggggataagg gaggtccgcc
     1021 aggagcgaga cgagcatcgc ctcgaagggt atatggttgt aggggagcac cttgcctggg
     1081 tcaagctggc tgtacggtgc gagcacgcgg ttctgcgcgg cacgctgcgc ttccaggtcg
     1141 tacagcaacg gcccgttccc atcgcggatg atgagtccgc ccgtcagggt cgagacgaaa
     1201 tcggtgcgcc ggacgtagcc ttcgggtccg ccgaactgct gcaccgtccc cgcccacatc
     1261 aagaggtagc tgacgaccag ccccacagtc agggcaacgg ccaccttccc gacgagagtc
     1321 caccgccccc ggtcccgcgc gcctggcccc tggcccctgg ctcctggccc ctggctcaaa
     1381 agcctgcgct caagctctac ggggtcgtcg gtaggctcct ggctagcgcg caagtacgcc
     1441 ctccacttgg ttggcgcttg ccggtgtagc gcggcgctgc caggcgtcaa ttacggcttg
     1501 caggaagagc ctgccgtctt cgccgcccag aatggcttcg gctgcgcgct cagggtgcgg
     1561 catgaggccg agcacattgc cctcgcgatt gcagatgccc gctatggcgt cggaagagcc
     1621 gttggggttg gcggctgggt caagcgaacc ttcaggcgtg ctgtagcgca aaacaacctg
     1681 cccgctctca ttcagttccg cgagcgtttg ggggcttgcg aagtagcggc cctcaccatg
     1741 ggcgatgggc agccgccaga ccgtcccagg ctgaacaccc tcaaggagag cgcagccaga
     1801 tggctcgacg cgcacatgga cccaccggca ggagaacgcc agttcgctgt tacgcatcag
     1861 cgcgccggga aggagatgcg cctcggtgag cacctggaag ccgttgcaga tgcccagcac
     1921 ccacccaccc tcctgggcgt gcctcctgac ggcttctatc acgggggtga aacgcgctat
     1981 cgcccccgcc cgcagatagt cgccataaga gaagccgccg gggaggagca caagggcgta
     2041 gcggctgacg tcggtcgcgg tgt
//
LOCUS       DASRWZ010000358         7860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1126919, whole genome shotgun sequence.
ACCESSION   DASRWZ010000358 DASRWZ010000000
VERSION     DASRWZ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126919"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /gene="atpD"
                     /locus_tag="VFH60_05525"
     CDS             complement(<1..1309)
                     /gene="atpD"
                     /locus_tag="VFH60_05525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET6313276.1"
                     /translation="MVATSTRGATGRIVQITGPVVDIEFPPDQLPEIYNAVEIPITSE
                     TAREGVGDGESGSAASGRVICEVQQHLGNDWVRSVSMTTTDGLRRGMPAYDTGTSISV
                     PVGEATLGRIFNVTGDTIDERGPVDTQERYSIHRPAPRFEDQSTVAQIFETGLKVIDL
                     IAPFSKGGKIGVFGGAGVGKTVVITELINNVAKQHGGFSVFCGVGERTREGTQLYKEM
                     HEAGVLDKMVMVFGQMNEPPGARLRVGLTGLTMAEYFRDTHGTDLLVFIDNIFRFVQA
                     GSEVSALLGRMPSAVGYQPTLAQEMGQLQERITSTKNGSITSVQAIYVPADDYTDPAP
                     ATTFAHLDATINLERNLAAQALFPAVDPLASTSRILDPRIVGQEHYDVARNVQRVLQR
                     FKDLQDIIAILGIEELSEEDKLTVARARRIQRFLSQPMFTAAAF"
     gene            complement(1365..1871)
                     /locus_tag="VFH60_05530"
     CDS             complement(1365..1871)
                     /locus_tag="VFH60_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313277.1"
                     /translation="MAKIYHAKPTSTKLLEGAIAGLLGGLAFALVMLIADFLTPQRPW
                     WSTFWLIGTILTGTPVAEINRFNIVNIGLGLLLHLLVFALLGVGLVQYVPLFRRFKID
                     RILGGAIYGVVVFLSVYWYLVSFFFPGVSANLNLIALFIASVVGGAVMGWWLKRAAAP
                     AKAAPNAT"
     gene            complement(1973..2893)
                     /gene="atpG"
                     /locus_tag="VFH60_05535"
     CDS             complement(1973..2893)
                     /gene="atpG"
                     /locus_tag="VFH60_05535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET6313278.1"
                     /translation="MPSTREIRRRIRSAKNISQITRAMEMVAASRMKRATQQALSGRP
                     YSERIGDLIRNLSRRLDLSDTETLNPLLLSRPIRNITVIVFTADKGLAGALNTNVLRR
                     TTRFILNEVNGVPVNVVAVGRKGRDFMIRYGRPLLAEFTNIGDRPTIQDIRAISRVVS
                     DEFVAGRTDAVYLIYTDFVNTLVQRPNVYRLLPIDVSHVGEEGALPGMNVDEQAEGGH
                     TEVEYEYEPNDRAVLAQLLPRFVEVRLYQALLEHLASEHSSRMVAMRNATQNAKEMIS
                     DLTLSYNKLRQANITSEIIEVSSGAAAQAR"
     gene            complement(2993..4525)
                     /gene="atpA"
                     /locus_tag="VFH60_05540"
     CDS             complement(2993..4525)
                     /gene="atpA"
                     /locus_tag="VFH60_05540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET6313279.1"
                     /translation="MALRAEEISKLISEEIQNFGQPSRTVNVGTVVEVGDGIARVHGL
                     SNVMSSELVEFEVQGNEGTQIISGLALNLEEDSVGVVILGQWTGIEEGSTVRTTGRIV
                     EVPVGDALLGRVVNALGQPIDGKGDLPANMGTRPVERIAPGVVYRQNVDTPVQTGIKA
                     IDSMIPIGRGQRELVIGDRQTGKSAIALDTIINQKGGDMLCIYVAIGQKASQVAQVVG
                     ILEDAGAMEHTIVVAATAADPAALQYLAPYAGCAMGEEFMETGRDCLIVYDDLSKHAW
                     AYRQISLLLRRPPGREAYPGDVFYLHSRLLERAARLSEERGGGSLTALPFIETQANDV
                     SAYIPTNVISITDGQIFLEPDLFYAGIRPAINVGVSVSRVGGDAQIKAYRQVAGRMKL
                     DMAQFRELAAFAQFGSDLDKATKAQLDRGLRIQEVLKQPQYRPLKVAQQVSIIYAVTN
                     GYMDDVPVEQIRSWEEQFHSFMGTAHPEVLETITTEKRLSDETTAALVTAIEEFKRTV
                     SL"
     gene            complement(4615..5154)
                     /gene="atpH"
                     /locus_tag="VFH60_05545"
     CDS             complement(4615..5154)
                     /gene="atpH"
                     /locus_tag="VFH60_05545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET6313280.1"
                     /translation="MAQRGGAARRYAQAVLDLAKQDNSLDAWLNDLKLLNRMFGTERA
                     VEALEDPKLTHEAQEEIVQRVVPKEVTNPLVRNFMLLLVKRQRLALLPRIVEVFQEMY
                     NKERGIVVADVTTAVPLDEAHQRRVAEQLSGITGGKTIELRLHTDPSILGGMVARIGD
                     ELLDASVATRLATLAQRLT"
     gene            complement(5243..5755)
                     /gene="atpF"
                     /locus_tag="VFH60_05550"
     CDS             complement(5243..5755)
                     /gene="atpF"
                     /locus_tag="VFH60_05550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET6313281.1"
                     /translation="MFEQLGVLWGNLLWQMAAFGLLVFLLTRFAYRPIVKMLDDRASR
                     ISDSMAQAEQIRLDNQNASKRAQEILAEAQQQTREMLAQANQMSQRTIAAAQEEARQQ
                     REKILVDARAQIEADTQRAKEELRREVGRLAIMAASRVVGRSLDTQDHYRLVDEALAD
                     AEQTRGFGRG"
     gene            complement(5877..6149)
                     /gene="atpE"
                     /locus_tag="VFH60_05555"
     CDS             complement(5877..6149)
                     /gene="atpE"
                     /locus_tag="VFH60_05555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET6313282.1"
                     /translation="MLFGTLLQAANEAGVTDRGIKLIAAGLAIGLGALGPGIGLGVGT
                     FGAVQAISRNPDAEGSIRTAMILGLGFAEAVAIYALVVALVIVFVG"
     gene            complement(6282..7361)
                     /gene="atpB"
                     /locus_tag="VFH60_05560"
     CDS             complement(6282..7361)
                     /gene="atpB"
                     /locus_tag="VFH60_05560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET6313283.1"
                     /translation="MLGILFQTTEGEGEGIDLTSKHPPISIKAEVLFDIPVPFFGTLH
                     FTNSMLYTLIVIAVLVLFFALATRRMSLVPKGSQNFAEAIVEFLLGTTEGTAGRRVGR
                     RIFPLIATLFLFIIAANWSGLLPGVGTIGGCYHVPEGEDHALVTNVKTAAPAQGLALA
                     PASQDDHAEAGGGGLPTPTGHECPEGTHFVPFLRAPNADLNTTLGMAIIAVLVVQIAG
                     IASHGLGGYLKELVTPVFLAPIHIMGEISRIISLSFRLFGNIFGGEVLVTVMFVLLGS
                     VFAGFGAFIFLGIEVLFGFIQALLFSILTLVFISSAVAGHGGGPGHGDEEHGHGGSSA
                     HGDPSGTAPETPSDQATETSSHVGG"
     gene            complement(7467..>7860)
                     /locus_tag="VFH60_05565"
     CDS             complement(7467..>7860)
                     /locus_tag="VFH60_05565"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET6313284.1"
                     /translation="AHDMQDSGTKEQEPGGEVTGSGDVSSPLKDGGMAQSLAFAAQLG
                     FAIACPMVAFIGGGAWLDNRFDTVPWLLVAGILLGILAAAGTIYQVLRAQTNRLERSK
                     RPGAPYKVEGQVSDPGTKSADKRGRNGR"
BASE COUNT         1387 a         2507 c         2403 g         1563 t
ORIGIN      
        1 tgaaggcggc ggcggtgaac atcggctgcg agaggaagcg ctggatgcgg cgggcgcggg
       61 ccacggtcag cttgtcctct tcggaaagct cctcgatgcc caggatggcg atgatgtctt
      121 gcaggtcctt gaagcgctgc aacaccctct gcacgttcct ggctacgtcg tagtgctcct
      181 ggcccacgat gcgcgggtcg aggatgcggg acgtggaagc gagtgggtcc accgccggga
      241 agagggcctg tgccgccagg ttgcgctcaa ggttgatggt ggcgtcgagg tgggcaaagg
      301 tggtagcggg tgccgggtcg gtgtagtcgt cggcgggcac gtagatagcc tgcaccgagg
      361 tgatggagcc gttcttggtg gaggtgatgc gctcctgtag ctggcccatc tcctgcgcga
      421 gcgtcggctg gtagcccacg gcggaaggca tgcgacccag cagcgccgac acctcggaac
      481 ccgcctgcac gaagcggaag atgttgtcga tgaacaccag caggtcggtg ccgtgcgtgt
      541 cgcggaagta ctcggccatc gtgaggcccg tcagccccac gcggaggcga gcgccgggcg
      601 gctcgttcat ctgcccgaac accatgacca tcttgtcgag cacgccggcc tcgtgcattt
      661 ccttgtaaag ctgcgtgccc tcacgggtgc gctcgcccac gccgcagaac acggagaaac
      721 cgccgtgctg cttggctacg ttgttgataa gctcggtgat aaccaccgtc ttacccacgc
      781 ccgcgccgcc gaacacgccg atcttgccac ccttcgagaa gggcgcgatc aggtcgataa
      841 ccttgaggcc cgtctcgaat atctgggcca ccgtggactg gtcctcgaag cgaggggcgg
      901 ggcggtggat cgaataccgc tcttgtgtat ccacggggcc gcgctcgtct atcgtgtcgc
      961 cggttacgtt gaagatgcgg cccagcgtgg cctcacccac cggcacggag atcgacgtcc
     1021 cggtgtcata ggcgggcatg ccgcggcgca ggccgtcggt ggtggtcatg gacacgctgc
     1081 gcacccagtc gttgccgagg tgctgctgca cttcgcaaat cacacggccg ctcgccgcgc
     1141 tgccgctctc gccgtccccc acgccctcgc gtgcggtctc cgaagtgatg ggaatctcca
     1201 ccgcgttgta tatctccggc aactggtcgg gcgggaactc gatgtccaca accggaccgg
     1261 ttatctgcac aattcttccc gttgctcccc tggtgcttgt tgctaccata atgcgatctc
     1321 tcctttgact agagaaccct tctctttctc ttcctcacaa aatgctaagt ggcgttgggt
     1381 gccgccttcg cgggggcggc cgcgcgcttg agccaccagc ccatcacggc ccctcccacc
     1441 accgaagcga taaacaatgc tatcaggttc aagttggcgg acacacctgg gaaaaagaag
     1501 ctcacgaggt accagtagac gctgaggaac accaccaccc cgtaaatcgc accgccaagt
     1561 atcctgtcta tcttgaaccg cctgaacaac ggcacgtact gcaccaggcc cacccccaac
     1621 agggcaaaca caagcaggtg cagcaacagc cccaatccta tattgacaat gttgaagcgg
     1681 ttgatctccg ctaccggcgt gccggtcaat atggtgccta tcagccagaa ggtagaccac
     1741 cagggacgct gcggggtcag gaagtccgcg ataagcatga ccagcgcgaa cgccagcccc
     1801 ccaagcaacc cagcaatcgc cccttcaagt agcttcgtgc tggtaggttt cgcatgatag
     1861 atttttgcca taacgcctaa ctcgcaatcg ttggtatgta accgccgcca cgctggtgct
     1921 caacctgagt tacgcgctac gtgttacctg ttactggttg cctcttgctc cgctaccggg
     1981 cctgcgccgc cgcaccgctc gacacctcga taatctcgct cgtgatgtta gcctggcgca
     2041 acttgttgta tgacagcgtc aggtccgaaa tcatctcctt cgcgttctgg gtggcgttac
     2101 gcatagccac catgcgcgag ctgtgctcgg atgcgaggtg ctccaacagc gcctggtaga
     2161 gcctcacctc aacgaagcgc ggcagcagtt gcgccagcac cgcccggtcg ttcggctcgt
     2221 actcgtactc gacctcggtg tgcccgccct ccgcctgctc gtccacgttc ataccgggca
     2281 gcgcaccttc ctcaccgacg tggctcacgt cgataggcag caggcggtag acgttcggcc
     2341 tctgcacgag cgtgttgacg aagtcggtgt aaatcaggta cacggcgtcg gtgcggcccg
     2401 ccacgaactc gtcgctcacc accctggaga tagccctgat gtcctgtatc gtgggcctgt
     2461 cgccaatgtt ggtgaactcg gcaagcagcg gccgcccgta gcggatcatg aagtcgcgcc
     2521 ccttgcgacc caccgccacc acgttcaccg gcaccccgtt cacttcgttc aagatgaagc
     2581 gagtggtgcg cctcagcacg ttcgtgttca acgcccccgc cagccccttg tcggcggtaa
     2641 agacgatgac cgtaatattg cggatgggcc gcgagagcag cagcggattg agcgtctccg
     2701 tgtccgacag atcgagccgc ctcgacaggt tgcgtatgag gtcgccgatg cgctccgagt
     2761 agggccgtcc cgacagcgcc tgctgcgtag cccgcttcat gcgcgaagcc gccaccatct
     2821 ccatagcccg cgtgatctgc gaaatgttct tagccgacct gattcgccgg cgtatctctc
     2881 ttgtactagg catattgccc cttcggggaa tttggaattt tggatttcgg attgcgaatt
     2941 tgctgcatac ctactaaatt cgcaattcga aatccaaaat tccaaattcg tcttacaggc
     3001 ttacggtgcg cttgaactcc tcgatggccg tcacgagggc tgccgtggtc tcatcggaga
     3061 gccgcttctc ggtggtgatc gtttcgagga cttcggggtg agcggtgccc atgaagctgt
     3121 ggaactgttc ctcccagctt cggatttgct ccaccggcac gtcgtccatg tagccgttag
     3181 tcaccgcgta gatgatggag acctgttggg ccaccttcag cgggcggtac tgcggctgct
     3241 tgagcacttc ctggatgcgg aggccgcggt cgagctgtgc cttggtcgcc ttgtcgaggt
     3301 cggagccgaa ctgggcaaag gccgcaagct cgcggaactg ggccatgtcg agcttcatgc
     3361 gcccggccac ctggcggtac gccttgatct gcgcgtcgcc acccacgcgg cttaccgaca
     3421 cacccacgtt gatggcgggg cggataccgg cgtagaagag gtcgggctcg aggaatatct
     3481 ggccgtccgt gatcgagatc acgttggtcg ggatgtaagc cgacacgtcg ttggcctgcg
     3541 tctcgatgaa aggcagggcc gtaagcgagc caccgccgcg ctcttcgctc aagcgggcgg
     3601 cgcgctctag cagccgggag tgcaggtaga acacgtcgcc ggggtacgcc tcgcgtcccg
     3661 gagggcggcg caggagcagc gaaatctggc ggtaggccca ggcgtgcttg ctcaggtcgt
     3721 cgtacacgat gaggcagtcg cggcccgtct ccatgaactc ctcgcccatc gcgcaaccgg
     3781 cgtaaggagc aaggtactgg agggctgccg ggtcggcggc ggtggccgca accacaatag
     3841 tgtgctccat cgcgcccgcg tcctcgagga tacccaccac ctgggccacc tgcgaggcct
     3901 tctggcctat ggcgacgtag atgcagagca tgtcgccgcc cttctggttg ataatcgtgt
     3961 ccagggcgat ggcggacttg ccggtctgcc ggtcgccaat caccagttcg cgctggcccc
     4021 ggccaatcgg gatcatggag tcgatggcct tgatgccggt ctgcacgggc gtatcgacgt
     4081 tctggcggta caccacgccg ggcgcgatgc gttccacggg gcgggtgccc atgttggcgg
     4141 gcaggtcgcc cttgccgtcg ataggctggc cgagggcgtt caccacgcgg cccagcaggg
     4201 cgtcgcccac cggcacctcg actatgcgcc cggtggtgcg caccgtggaa ccctcttcga
     4261 tgccggtcca ctgccccagg ataacgacac cgaccgagtc ctcttccagg ttgagggcca
     4321 gaccgcttat tatctgcgtg ccctcgttcc cctgcacctc gaactcaacc agctcggagg
     4381 acatgacgtt cgacaggccg tgcacgcggg cgataccgtc accgacctcc actaccgtac
     4441 cgacgttcac cgtgcgggaa ggctgcccga agttctgtat ctcttcgcta attagcttgc
     4501 ttatctcttc tgcgcgtaga gccataatat tctccctcta gtacttccac ttccactacc
     4561 gggtccggct atcgacgtta tgctctcggc tcgtgcgtga gcctgacagg tctgctaggt
     4621 gagcctctgg gccagggtcg caaggcgggt cgccacgctg gcatcgagaa gctcatcgcc
     4681 tatgcgagcc accatgccgc ccaggatgct ggggtcggtg tgcaggcgaa gctcaatcgt
     4741 cttgcccccg gtgatgcccg aaagctgctc ggccacccgc ctctggtggg cctcgtccag
     4801 cggcacggcc gtggtaacgt ccgccaccac gatgccccgc tctttgttgt acatctcctg
     4861 gaacacctca acgatgcgcg gcagcagggc caaccgctgc ctcttgacga ggagcaacat
     4921 gaagttgcgc acgagcgggt tggttacctc tttggggacc acccgctgca ctatctcctc
     4981 ctgggcctcg tgcgtcagct tggggtcttc cagcgcctcc acggcccgct ccgtgccgaa
     5041 catgcggttc agcagcttga ggtcgttaag ccaggcgtcg aggctattgt cttgttttgc
     5101 caggtcgagc acggcctgcg cgtacctgcg cgccgctcct ccacgttgtg ccacagtctt
     5161 ctccgatttt ggattttaga ttttagattt tggattgggt tctaagcaat ctaaaatcca
     5221 aaatctaaaa tctaaaatgt gatcatcctc ttccgaagcc gcgggtctgc tcggcgtcgg
     5281 ccagggcttc gtctaccagg cggtagtggt cctgggtgtc gagcgagcgg cctactacac
     5341 ggctggcggc catgatcgcc aggcggccca cttcgcggcg cagctcttcc ttcgcccgct
     5401 gggtgtcggc ctctatctgg gcgcgggcgt ccaccagtat cttctcgcgc tgctgccggg
     5461 cttcttcctg ggctgcggca atcgtgcgct ggctcatctg gttggcctgg gccagcatct
     5521 cgcgggtctg ctgctgcgcc tcggccagta tctcctgggc gcgcttggac gcgttctggt
     5581 tgtcgaggcg tatctgctcg gcctgcgcca tgctatcgct aatgcgactc gcccggtcgt
     5641 ccagcatctt gacaatgggg cggtaggcga aacgcgtgag caggaacacc agcagcccga
     5701 acgccgccat ctgccacagc aaatttcccc acagcacgcc tagctgttca aacatgtcgt
     5761 ggtcccttca catagaacgg gtgtctggct ccgcaaacca ccggtcctca cccctacccc
     5821 ttcccaatcg aacgctggga ggggtaagga cgtgacacgg tggagattgg ccgagcctaa
     5881 cccacgaaga cgataaccag ggcgacaacg agcgcgtaaa tagcgaccgc ttcagcgaaa
     5941 ccaaggccca ggatcatcgc ggtacggatc gagccttcgg cgtcggggtt gcgcgagata
     6001 gcctgcacag cgccgaacgt accgacacca agaccgatgc cggggccaag agcgcccaga
     6061 ccaattgcca ggcccgccgc tatcagcttg ataccgcggt ctgtaacgcc cgcctcgttg
     6121 gccgcctgta gcaacgtacc gaataacatc aacaactcca ctgcttttcc tcctaggtgt
     6181 acttggttgg tatttccact cctcgtgggg ccttgcggcc ttgcatgagc cggaagcgtt
     6241 acgcttccat ttctcccaaa atgagattgt gcctctttac gtcagccccc gacgtggctg
     6301 gaggtctccg ttgcctggtc ggaaggagtc tcaggggctg tgcctgaggg gtcgccatgt
     6361 gcggagctgc caccgtgccc gtgctcctcg tcgccgtggc caggcccgcc tccatgccct
     6421 gctaccgcgc tcgatatgaa caccagggtg agtatcgaga agagcagcgc ctgtatgaag
     6481 ccgaacagca cctcaatgcc gaggaagatg aacgccccga agccagcgaa gacgctgccc
     6541 agcagcacga acatcaccgt caccagcact tcgccgccga agatgttgcc gaacagacgg
     6601 aaggagagcg atatgatgcg agaaatctca cccatgatgt gtatgggcgc gaggaatacc
     6661 ggcgtcacca gctccttcag gtagccaccc aggccatgcg aggcgatacc ggctatctgc
     6721 accaccagca ccgcgatgat tgccatgcca agtgtggtgt tgaggtcggc gttaggggcg
     6781 cgcaagaagg gtacgaagtg ggtgccttcc gggcattcgt gccccgtggg agtgggcagc
     6841 ccgcccccgc ctgcctcggc atggtcgtcc tggctcgccg gggcaagggc cagtccctgg
     6901 gcgggggcgg cggtcttcac gttggtcacg agggcgtggt cctcgccttc cgggacgtgg
     6961 tagcagccgc ctatcgtgcc gacgcccggc aggaggccgg accagttggc ggcgatgatg
     7021 aacaggaaca acgtggctat cagcgggaag atgcggcgtc cgaccctgcg ccccgccgtg
     7081 ccctcggtgg tgccgagcag gaactcgaca atcgcttcgg cgaagttctg cgagcctttg
     7141 gggaccaggc tcatgcggcg cgtcgcgagc gcgaagaata acaccaggac ggctatcacg
     7201 atgagcgtgt atagcatcga gttggtgaaa tgtaaagtgc cgaaaaaggg tacggggatg
     7261 tcgaaaagaa cttcagcctt gatgctgata ggggggtgct tggacgtgag gtctatgcct
     7321 tcgccttcgc cttcggtcgt ctggaaaaga atacctaaca tgaagggctt cttcctcttc
     7381 gaagtgtacc ggccctctgc cggtcaacaa ttggcctgct ggggggtcgc tttgccccat
     7441 gttgttatgg attttgcggc ccttgcttac ctaccgttcc gaccgcgctt atctgcactt
     7501 ttcgtgccgg ggtccgagac ctgcccttcg actttgtagg gcgctcctgg ccgcttgctc
     7561 cgttcgagac ggttggtttg cgctcgcagc acctggtaga tggtgcccgc cgctgccagg
     7621 ataccgagca ggatgcccgc gacaagcagc cagggcacgg tgtcgaacct gttgtcgagc
     7681 cacgccccgc cgccgatgaa cgccaccatc gggcaggcaa tcgcgaaacc tagctgcgcg
     7741 gcaaacgcca acgactgggc catgccgccg tccttgaggg ggctgctaac gtcgccgcta
     7801 ccggtgactt cgccgcccgg ctcctgctcc ttagttccgc tatcctgcat gtcgtgtgcc
//
LOCUS       DASRWZ010000359         1905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1058337, whole genome shotgun sequence.
ACCESSION   DASRWZ010000359 DASRWZ010000000
VERSION     DASRWZ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058337"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..>1905
                     /locus_tag="VFH60_05570"
     CDS             151..>1905
                     /locus_tag="VFH60_05570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6313285.1"
                     /translation="MSNIDTAVATGAGSGTPIAAGVAASRVASLLVPARLLWGVVLLG
                     GLLRFYGYDRLSLWLDEGYTVMFSRLPWDQVLGLHGAYDSHPPLYYALVKLVSLVAPE
                     ASSGRLLSVVAGTLSIPVVYALGRKLFTEWAGLVASVALALSPLHVWYSQEARQYAIA
                     FLLVALAYYALLSFYREPRWGWALLYGATTLVSMYIDYSPLYALLPQLVILSLVLRKH
                     GRRVLPLFASGALAVVGYLPWVPQLLATGRLGDQSRSYLAATEYRVYASIRSILGFAE
                     NGNYFYSERQVPWEQWPLLRGAALVLVSATILLGVLWLWRRSRLGLAFVASLGAGTVL
                     VAIGISMLSPGYADRTVLYATLGWSLLLGALVAYPLPRRLSLPRWLSLGCVLLVFAVS
                     LYTVYFYADKEHWRDLARDTNAAASSGKLLLTYPGPGAEALIGVYQPNALLGRHINLE
                     WPGQLPAVAQQIDPQRDDVLWLSYLDINETQLVRDWLSGLGYERVLRNEHSRRLSLDM
                     YVLPGALRGTPMPLNGQFRGAGAQAEGWSLPPEGVEFSPVGEGREMSLVGDAKVERSA
                     YASLPARPNATYVLHAEAR"
BASE COUNT          258 a          609 c          631 g          407 t
ORIGIN      
        1 ctctttgcgc cccttcggct gtgctcaggg catgctttgc ggtttcgtct ctcctctttc
       61 gtactataat ggaagctgtt gtagtaggac gtttttggct catataattc accgggcccg
      121 ggtatgcctc gccctcagac accacactcc atgtccaaca tcgatacagc agttgctaca
      181 ggtgcgggta gcggcacccc catagcagcg ggcgtagcgg cctcgcgggt cgcttctctc
      241 ctggtgcccg cgcggctgct ttggggcgtg gtgctgctgg gcggcttgct gcggttctac
      301 ggctacgaca ggctgagcct gtggctggac gagggctata cggtgatgtt ctcgcgcctg
      361 ccgtgggacc aggtgctggg cctgcacggg gcttacgact cccaccctcc cctctactac
      421 gcgctggtca agctggtgtc gttggtagcc ccggaggcga gctcggggcg gctgttgagc
      481 gtcgtggcgg gcacgctgag catcccggtg gtttacgcgc tgggccgtaa gctgttcacc
      541 gagtgggcgg gcctggtcgc gagcgtggcg ctggcccttt ctccgctgca cgtctggtac
      601 tcgcaagagg cgcggcagta cgctatagcc ttcctgctgg tggccctcgc ctactatgcc
      661 ctcctgtcgt tttaccgcga accccggtgg ggctgggcgc tgctttacgg cgctaccacg
      721 ctggttagca tgtacatcga ctatagcccg ctatatgcgt tactgccgca acttgtgatt
      781 ctctcattgg tcttgaggaa acacgggcgg cgcgtgctcc ccttgttcgc atcgggcgcg
      841 ctggcggtgg ttggctacct gccgtgggtg ccgcagttgc ttgccaccgg cagactgggc
      901 gaccaatcgc gctcctacct ggccgctacc gagtaccgcg tgtacgcctc cattcgctcc
      961 atcctgggct tcgcggagaa cggcaactat ttttatagcg agaggcaagt gccctgggag
     1021 cagtggccgc tgcttcgcgg ggcggcgctc gtgctggtga gcgccactat ccttttgggc
     1081 gtgctgtggc tgtggcggcg ctcgcgtctc ggcctggctt tcgtggccag cctgggcgcg
     1141 ggcaccgtgc tggtggcgat tggcatcagc atgctaagcc ccggctacgc agaccgtacc
     1201 gtgctgtatg cgacgctcgg ctggtcgctg ttgctgggcg cgcttgtggc ttacccgctg
     1261 ccgcgccggc tctcgctgcc ccgatggctg tccctggggt gcgtgctgct ggtgtttgcc
     1321 gtttcgctgt acaccgtcta cttttatgct gataaggagc actggcgcga cctggcccgc
     1381 gatacaaacg ccgccgcctc ttcgggcaag ctgctgctga cctaccctgg acccggcgct
     1441 gaagccctga taggcgttta ccagcccaac gcgctcctgg ggaggcacat caacctggaa
     1501 tggcccggcc aactgcccgc cgtcgcccag cagatagacc cacagcgaga cgacgtgctg
     1561 tggctctcct acctggacat caacgagaca cagttggtgc gcgactggct ctccggcctc
     1621 ggctacgagc gggtgctacg caacgagcat tcccggcgtc tgtccctgga catgtacgtg
     1681 ctgcccggcg cgctaagggg tactcccatg ccgctgaacg gccaattcag gggagcgggc
     1741 gcgcaggctg agggctggag cctgccgcct gagggtgtgg agttctcgcc cgtgggtgag
     1801 gggcgcgaga tgtcactggt aggtgacgct aaggtagagc gcagcgccta cgccagcctc
     1861 cccgcgcgcc ccaacgcgac atacgtgctg cacgcggagg cgcgg
//
LOCUS       DASRWZ010000360         5696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_579571, whole genome shotgun sequence.
ACCESSION   DASRWZ010000360 DASRWZ010000000
VERSION     DASRWZ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579571"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1183
                     /locus_tag="VFH60_05575"
     CDS             221..1183
                     /locus_tag="VFH60_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6313286.1"
                     /translation="MAEVAGAPVLGIDIGGTKLAAGVVGGGGRVLSYVREATPTGVDG
                     EALLAFVLRLARRALDESSTGSAPVGVGIGCGGPMVFPEGIVSPLHIPAWQDFPLRAR
                     VESALGLPTVLDNDAKAFALGEALFGAGRGARCMLGMVVSTGVGGGIVAEGRLFDGAS
                     GNAGHVGHVLVSKDGPRCYCGGHGCLTVYASGTGLAARAREALEQGIPSSLASLDVEQ
                     VTGQAVAEAAAAADPLALDLMRGAGQALGMGIASVANLLDLDRVVIGGGVAQSGDLLF
                     NPLLEELRRRATLAFSRDVEVRLAQLGQEAGVVGAAGLVFQRVK"
     gene            1488..2957
                     /locus_tag="VFH60_05580"
     CDS             1488..2957
                     /locus_tag="VFH60_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6313287.1"
                     /translation="MNEKSKMTGLRRIAALLLLSSMVLSACDSGTPATPTSPAPTNTT
                     VAPAVDATNTTTTEAAPTNTVSAPADTPEPAGNAPRLNKDVSGDVEFWHHWSSPVRRN
                     AIRRVIAACAVELPNIKVTEVAKPFGEVWTANTAAVAAGSGMPDVLVSDRPKLPQDAA
                     NQIYTDLNEWAVRDNVTGDQFWPFTWQQTLYQDHTYGIPFETDVRVLFYNKVAFTEAG
                     LDPANPPKTWAELEAAADKLDKKNADGSLARIAFFPLQGNASPEILGYTNGVDWVKDD
                     GTLEINTPEAAETLDWVKKWVDRYGGWETIQAFRAGFQSAPNDAFMSGKVAMIVDVNG
                     YASQLNFYNPQVEKGDGSGRTRLDWGVSDIPYNKEKASYSGGFSFSIPRGADGAEAAW
                     EFIKCATGKEGAASWSRDTYAMAANLEAANDPVLGSDPIWKFFLSAMEYSRSGNYLEA
                     YPNWGEQLSQRYEKLWKGEITSQQLLQEAEQGVKDEIGQ"
     gene            3113..4096
                     /locus_tag="VFH60_05585"
     CDS             3113..4096
                     /locus_tag="VFH60_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6313288.1"
                     /translation="MALASSSQPAPKPRWSFTPKAREGLTGWLFALPWILGFFVFTLG
                     PMLFAGYASFTRYNITTPPEWVGTNNYERLFRDDRFYTGLGNTLWMVAVKVPIVIVLA
                     LIIAMLLNIELPGSRFFRSVFYMPNVLSGAPAVFLWLWMLSPTGLINNFLGLFGVKGP
                     AWWSNADWTKPGLVVMGTWWIGGNILILLAGLKGIPNDLYEAASIDGASGWTRFRFIT
                     LPMLSPTIFFQIVTGIIGTFQIFTTAYLITSSSSTGTSSAESLANGGPGQSLLFYVLY
                     LYNRAFGKQGIGFQMGYASAMAWILFIIILAVTLVQLWLARRWVHYETEGK"
     gene            4099..5037
                     /locus_tag="VFH60_05590"
     CDS             4099..5037
                     /locus_tag="VFH60_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6313289.1"
                     /translation="MATIIEQQPTIAPVIEDTAKKERLQVPRKPMSPGKIAAFIVLCG
                     MSFLFIMPFVWMLSTSLKDFGDLAGSNWIPNPITWDNYVKAFSYGMWPRWAMNSIIIT
                     GVSVFGTVLSTSIIAYSFARLRWPGRDQVFALVLATMMLPGVVTMIPQYILFSKLPAF
                     GFMGSETWVNTFLPLTVPAFTGNAFYIFLLRQFMRGIPMELSEAARIDGASELRIWWS
                     IVMPLTKPALAAVAIFTFQFVWEDFQGPLLYLPSEQNYTLQIALRFFEAAAGGAPAWN
                     WMMAASLVVMLPVLIIFILFQRYFIEGVTLTGMGGR"
     gene            5195..5581
                     /locus_tag="VFH60_05595"
     CDS             5195..5581
                     /locus_tag="VFH60_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313290.1"
                     /translation="MSLFDEQGKMRDNKLDGLVDNLPSAGQVGDSGTEPSDFDSSLDE
                     SLVNDIGFIAAGQTAGNALGSSGTSSAPSAGAAGAAAIAGGTAYALEDDIAEEGSANS
                     VDNADAPDDFTGAGAEAADTDPTDRL"
BASE COUNT         1095 a         1716 c         1818 g         1067 t
ORIGIN      
        1 agcgaagtga gaaatctcgt ccttcagcac ccaagtatcg cctttcttta cgttagaggc
       61 ccatgagaaa agggaaacat ccaggtgagg gacgagattc ttcgctgcgc tcagaatgac
      121 aagggcgggg catagtcctg ctattccaat ttattcagaa tggcaaagtg ggcgatgatt
      181 ccgagttgct ttggcagagt cgtgttgggg aggtagcttg atggcggaag tagcaggcgc
      241 tccggtcctg ggcatcgata tagggggcac aaagctcgcg gcgggcgtag tgggcggtgg
      301 cgggcgggtg ttgtcctacg tgcgggaggc cacacccacc ggcgtagatg gggaggccct
      361 cctggctttc gtgttgaggc tggcgcggcg ggcgctggat gagagtagca ctggcagtgc
      421 gcccgtgggt gtcgggatag gctgtggggg gccgatggtc ttccccgaag ggattgtctc
      481 gccgctgcac atcccggcct ggcaggactt tccgctacgg gcgcgcgtcg agtcggcgct
      541 tggcctgcct accgtgctcg acaacgacgc caaggcgttc gcgctggggg aggcgctgtt
      601 cggggcgggg cgcggtgcgc ggtgcatgct gggcatggtg gtatccaccg gcgtgggcgg
      661 gggcatcgtg gcggaggggc ggctgttcga cggggccagc ggcaacgcgg ggcacgtggg
      721 ccacgtactg gtgtcgaagg acggcccgcg ctgctactgt ggcgggcacg gctgcctcac
      781 cgtctacgct tctggcaccg ggctggctgc gcgagccaga gaagccctgg agcagggcat
      841 cccatccagc ctggcctctc tcgacgtgga gcaggtgacc gggcaggccg tggcagaggc
      901 cgccgccgcc gccgaccccc tggcgctgga cctcatgagg ggggccgggc aggcgctcgg
      961 catgggcatt gccagcgtcg ccaacctgct ggacctcgac cgcgtggtga taggaggcgg
     1021 ggtggcgcag agcggcgatt tgctgttcaa cccgctgctg gaggagttac ggcggcgggc
     1081 cacactggcg ttctcgcgag acgtggaggt acgtctcgcc cagttggggc aagaagcggg
     1141 ggtagtgggc gcggcaggtc ttgtatttca gcgcgttaag tgagatattt gtctgtgatt
     1201 agtgcgtaaa ttgcgttgac aagcggcact gcaaacattt atcatactac ccgacgccgt
     1261 aatgcgtgaa ggaggtgagt ccccacacaa cctctgcata catgccggtc tgcccttata
     1321 cggccggcaa tacggcgcga ctcgttcgcg atcacgggag cacgacgctc cctgcctgac
     1381 tgggtggaaa gggggtgagc cgctgatacc aactccaggc ctgttctcaa gcctcaacag
     1441 acgcgcagat atttcccaac taactttcca gtaagaggag gaaagctatg aatgaaaagt
     1501 caaagatgac tgggttgcgc cgtattgccg cgctgctgct ccttagcagc atggtgcttt
     1561 cggcctgcga ctccggcact cctgctaccc ctacgtcccc tgcgccaact aacaccaccg
     1621 tcgcgccggc cgtggacgcc accaacacca cgacgaccga agcggcccct accaacacag
     1681 tctcggcacc tgccgatacc cctgaacccg cgggcaacgc ccccaggctc aacaaggacg
     1741 tttcgggcga cgtagagttc tggcaccact ggtcctcccc ggtgcgccgc aacgccatca
     1801 ggcgtgtgat cgcggcctgc gccgtggagc tgcccaacat caaggtcacg gaagtcgcca
     1861 agcccttcgg tgaagtttgg accgccaata ccgccgccgt agccgcgggt tcgggcatgc
     1921 ccgacgtgct agtgtcggac cggcccaagc tgccgcagga cgcggccaac cagatctaca
     1981 cggacctgaa tgagtgggcc gtccgcgata acgtcacggg cgaccagttc tggcctttca
     2041 cctggcagca gaccctctac caggaccaca cctatggtat ccccttcgag accgacgtgc
     2101 gggtgctctt ctacaacaag gtagccttca cggaggccgg cctcgacccc gccaacccgc
     2161 ccaagacctg ggctgagctg gaagccgctg ccgacaagct ggacaagaag aacgcggatg
     2221 gctcgttggc ccgcatcgcc ttcttcccgc tacagggtaa cgccagcccc gaaatcctgg
     2281 gctacaccaa cggcgtcgac tgggtcaagg acgacggcac gctggagatc aacactcccg
     2341 aagcggccga gacgctggac tgggtgaaga agtgggtaga ccgctatggt ggctgggaga
     2401 ccatccaggc cttccgcgcc gggttccagt cggcccccaa cgacgccttc atgtcgggca
     2461 aggtagccat gatcgtggac gtgaacggct atgcctcgca gctgaacttc tacaacccgc
     2521 aggtcgagaa gggcgacggc agcggccgca cccgtctaga ctggggcgtg agtgacatcc
     2581 cgtacaacaa ggaaaaggcc tcctacagcg gcggcttctc cttctcgata cctcgcggcg
     2641 ctgacggtgc cgaggcagcc tgggagttca tcaagtgcgc cacgggcaag gaaggcgcgg
     2701 cctcctggtc ccgcgacacc tacgctatgg ccgccaacct cgaagccgcc aacgacccgg
     2761 tgctaggctc cgacccgatc tggaagttct tcctgagcgc gatggagtac agccggtcgg
     2821 gcaactacct ggaagcttat cccaactggg gagagcagct aagccagcgc tacgagaagc
     2881 tctggaaggg cgagataacc tcgcagcagc ttctgcaaga ggctgagcaa ggtgtcaagg
     2941 acgagatagg ccagtaagca gtagcggcca ggtagcctct ggttagtggc tgtgaatcgg
     3001 gggcaggcaa tcagtagcga tagctgcctg gtgccggccc ccggtcgcat cccgaccact
     3061 tagtgctgct actgctactg ctgccgctgc tcagccccga agggaggttg ttatggcact
     3121 ggcatcttcg agccagccgg ccccgaaacc ccggtggtct ttcactccca aagcgcgaga
     3181 aggccttacc gggtggctgt ttgccctgcc gtggatactc ggtttcttcg tcttcacgct
     3241 tggaccgatg ctcttcgcgg gctacgctag cttcacgcgc tacaacatca ccactccccc
     3301 ggagtgggtg ggaacgaaca actacgaaag gctcttcagg gacgaccgct tctacacggg
     3361 gcttggcaac accctgtgga tggtagccgt caaagtgccc atcgtgatcg tgctggcgct
     3421 catcatcgcg atgctcctga atatcgagct accagggtcg aggttcttcc gctccgtctt
     3481 ctacatgccc aacgtgctgt cgggcgcgcc cgcggtcttc ctgtggctgt ggatgctttc
     3541 gcccaccggc ctgatcaaca acttcctggg actctttggc gtcaaggggc cggcatggtg
     3601 gtccaatgca gactggacga agccgggact tgtagtcatg gggacgtggt ggattggcgg
     3661 taacatcctg atcttgctgg ccgggttaaa aggcatacca aacgacctgt atgaagcggc
     3721 ctctatagac ggagcgagtg ggtggacccg cttccgcttc atcaccctcc cgatgctctc
     3781 gcctactatc ttcttccaga tagtcacggg catcataggc acgttccaga ttttcaccac
     3841 ggcttacctg ataaccagtt ccagcagcac cggcacatcc agcgccgaat cactcgcgaa
     3901 cggagggccg ggccagtccc tgctgttcta cgtgctctac ctgtacaatc gcgccttcgg
     3961 caagcaaggt atcggcttcc agatgggtta cgcctcggct atggcctgga tactgttcat
     4021 catcatactg gcggtcacgc tggtccaact gtggctcgcc aggcgctggg tgcactacga
     4081 gacggagggt aagtagagat ggcaaccata atcgagcaac aacccactat agcccccgtc
     4141 atcgaagata ccgctaagaa agaacgcctg caggtgccgc gcaaaccgat gtcgccgggc
     4201 aagatagcag ccttcatagt gctgtgcggg atgagcttcc tctttatcat gccgttcgtg
     4261 tggatgctct ccacctcgct caaggacttt ggcgacctgg caggctcgaa ctggataccc
     4321 aatccgataa cgtgggacaa ctacgtcaag gctttcagct acggcatgtg gccccgctgg
     4381 gcgatgaact caatcatcat cacgggggtc tcggtgtttg gcacggtgct ttccacctcg
     4441 atcatcgcgt attcgttcgc gcggttgcgc tggccgggac gcgaccaggt gttcgcgctc
     4501 gtgctcgcaa cgatgatgct gcccggcgtg gtcacgatga tcccgcagta catcctcttc
     4561 tccaagctgc ccgcgttcgg cttcatgggt tcggagacgt gggtgaacac cttcctgccg
     4621 ttgactgtgc ccgccttcac cggcaacgcc ttttacatat tcctgctgcg ccagttcatg
     4681 cgcggcatcc cgatggagct ttccgaggcg gcccggatcg acggggcctc ggagctacgc
     4741 atctggtgga gcatcgtaat gcccctcacc aagcccgcgc tggccgccgt ggctatcttc
     4801 accttccagt tcgtctggga ggacttccag ggtccgttgc tctacctgcc gagcgagcag
     4861 aactacacgc tgcaaatcgc cctgcgcttc ttcgaggcgg cggcgggtgg cgcgcctgcc
     4921 tggaactgga tgatggccgc cagcctggtg gtcatgctgc cggtgctgat tatcttcatc
     4981 ctgttccaac gttacttcat cgaaggcgtc acgctcaccg gcatgggcgg acggtaggat
     5041 aattacgatt gtgaatttcg aatttcgaat tgcgaattgc gaattcggag acgaaaacgc
     5101 aaagacgcaa aggacgcgca aagatcagga tagttgggtc aggtgctcgg cgtatttcgc
     5161 aattccaaat tcgtaaaggg gaaggaggag caagatgtcg cttttcgatg aacaagggaa
     5221 gatgagggac aacaagctgg atgggctggt cgataatcta ccgagcgcgg gccaggtggg
     5281 agacagcggc acggagcctt ccgactttga cagcagcctg gacgagagcc tggtgaacga
     5341 cataggcttt atcgccgcag ggcagaccgc cgggaacgcg ctggggtcga gtggtacgag
     5401 cagcgcccca agcgcggggg cggcgggtgc cgcagccatc gcgggaggca ccgcttacgc
     5461 cctggaggac gacatagccg aggaagggtc cgcgaacagc gttgacaacg ccgacgcccc
     5521 cgacgacttc accggcgcgg gcgcggaagc cgcggacacc gacccgaccg accggctata
     5581 aaacgtggcg cgtaagccgg ctggggtgta gtcagtatct ggcaggggaa cgccctgtgg
     5641 ggatagccct gtggctaaag ccgcgggctt acaaggggca aagtcccctt cgggga
//
LOCUS       DASRWZ010000361         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_305308, whole genome shotgun sequence.
ACCESSION   DASRWZ010000361 DASRWZ010000000
VERSION     DASRWZ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305308"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="VFH60_05600"
     CDS             <1..1463
                     /locus_tag="VFH60_05600"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET6313291.1"
                     /translation="VYAHLVRITEATGSGSQEAKVTLLAELFSRLQPLEAKHVARIIV
                     GRLRVGVGDPTLMDALSVAKAGDKSARPGIERAYNLSSDLGLVARTYYSSGLEGLEDI
                     RVTVGNPVRMALAERADSAEEILARLGKCAIEPKYDGFRMQVHKDGEQVSIFSRGLED
                     LSGMFPDVVAAVHAQIAARDAILEGEALAFEPDTGRYQPFQVTSQRRRIHNIEEMSAS
                     LPLRLLAFDLLLLEGNDLTRAPYTERRAVLEKAIRPIDGLGGIQVSTSLLTDDPEQIV
                     IFYEDSLEEGLEGIVAKRLDGTYEAGKRGFNWIKMKRSYQTQMRDTVDCAIVGYYYGK
                     GRRASWGIGSLLVSVYDPATDTLPTVTRVASGLSDEGWRDLCRRLEADRIEHHDPRVL
                     SVITPDLWVVPRLVVELQADEVSRSQMHPAGRTDSGPGYALRFPRIIKERPERTPEDA
                     TSVEEVIKLYNMQGKGDTRGKRKAAAEEGASPEVEA"
     gene            1499..1918
                     /locus_tag="VFH60_05605"
     CDS             1499..1918
                     /locus_tag="VFH60_05605"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6313292.1"
                     /translation="MGRLTRQTNISVPVEKVYGYVADPRNAPNYISSIVRIISGPEGQ
                     PQEGGAWRAEANFLGKRSVVALRLTRLTANQRVTFTVEGEPRAALTLTLAPIAKDTGT
                     NVSLLLDVPSVPEFLLNGLMGGLLTGDLARLRRALEG"
BASE COUNT          392 a          619 c          676 g          315 t
ORIGIN      
        1 aggtgtacgc ccacctggtg cggattaccg aggctaccgg ctctggcagc caggaagcca
       61 aagtaaccct gctcgccgag ctgttctcca ggcttcagcc gctggaggcc aagcacgtgg
      121 cccgcatcat cgtcggcagg ctgcgcgtcg gcgtgggcga cccgacccta atggacgcgc
      181 tgtcggtggc gaaggcgggc gacaagtcgg cgcggcccgg aatagagcgg gcgtacaacc
      241 tgtcgagcga cctgggattg gtggcccgca cctactatag ctccggcctg gaagggctgg
      301 aggacatccg cgtaacggta ggcaaccccg tgaggatggc cctggcggag cgggccgaca
      361 gcgcggagga gatactggcg aggttgggca agtgcgccat cgaacccaag tacgacggct
      421 tcaggatgca ggtgcacaag gacggcgagc aggtctcgat cttctcgcgg ggcctcgaag
      481 acctgagcgg gatgttcccc gacgtggtgg ccgcggttca cgcccagata gcagcgcgag
      541 acgccatcct ggaaggggag gcgctggcgt tcgagccgga cacggggcgg taccagcctt
      601 tccaggttac ctcgcaacgg cggcgcattc ataacatcga ggagatgagc gccagcctcc
      661 cgctccggct gctggccttc gacttgctgc tgctggaggg gaatgacctc actcgtgccc
      721 cctacacgga gcggcgggcg gtactcgaaa aggcgatccg gccaatcgac ggcctggggg
      781 gcatccaggt ctctacttcc ctcctgacag atgacccgga acagatagtg atcttctatg
      841 aggactcgct ggaggagggg cttgagggca tcgtcgcaaa gcgtctggac ggcacgtacg
      901 aggcgggcaa gcggggcttc aactggatca agatgaagcg ctcgtaccag acgcagatgc
      961 gcgacacggt ggactgcgcg atagtgggtt actactacgg taagggccgc cgggcctcgt
     1021 ggggcatagg ctctctgctc gtgtcggtct acgacccggc cactgacacc ctgccaaccg
     1081 tgacgcgcgt cgccagcggc ctctccgacg agggttggcg cgacctctgc cggcggctgg
     1141 aggccgaccg catcgagcac cacgacccgc gtgtgctctc cgtcatcacg cccgacctct
     1201 gggtggtgcc gcgcctggtc gtggagcttc aggccgacga ggtgagccgg tcgcaaatgc
     1261 acccggcagg gcgcaccgac tctggcccag gttatgcgtt acgcttccct cgtatcataa
     1321 aagagcgtcc tgagcgcacg ccggaagatg ccacctcggt agaagaggta atcaagctct
     1381 acaacatgca gggcaaaggc gacaccaggg gcaagcgcaa ggccgccgcg gaggaagggg
     1441 caagccccga agttgaggca tagccggtcc tcgaggcagg cacgaggagg tccacacatt
     1501 gggtcgattg acgcgccaga ccaacattag cgttccggtg gagaaggtct acggctacgt
     1561 ggccgacccg cgtaacgctc ccaactatat tagctccatc gttcgcatta tttcggggcc
     1621 ggaggggcag ccccaggaag gtggcgcgtg gcgagccgaa gccaacttcc tcggcaagcg
     1681 cagcgttgtg gccctacggc tgacccgtct caccgccaac cagcgggtga ccttcaccgt
     1741 cgaaggcgaa ccgcgcgccg ccctgactct gacgctggca cccatcgcga aagacaccgg
     1801 cacgaacgtc tcgcttttgc tggatgtacc cagcgtgcca gagttcctac ttaatggctt
     1861 gatgggcggc ctgctaacgg gagacctggc ccgcctcagg cgagcactgg aaggctagta
     1921 ccgagtagcg agtgccgagt accgagtacc gagtaccgag taccgagtac cgagtgctga
     1981 gtaaagagac ccgacgcgaa gg
//
LOCUS       DASRWZ010000362         5294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_785083, whole genome shotgun sequence.
ACCESSION   DASRWZ010000362 DASRWZ010000000
VERSION     DASRWZ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785083"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFH60_05610"
     CDS             complement(<1..322)
                     /locus_tag="VFH60_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313293.1"
                     /translation="MADERMRLMALVRSESSVGHAERNAAPYQLNTGDNEWFLANISC
                     QYACPAYTDVARYIAHIAAEDYESSYQIHLEDNVIPGILGRVCARPCEPACRRGRLDE
                     PIAIC"
     gene            complement(374..1009)
                     /locus_tag="VFH60_05615"
     CDS             complement(374..1009)
                     /locus_tag="VFH60_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6313294.1"
                     /translation="MIVRPFGGGPFNTTAYLVYDRQGGTGVIIDAPLGSTRKIVQVLR
                     EEQLQVPFVVSTHGHWDNIADNVAMCEATGAQLCAHFWDAARLANPGLTSDEELAAKI
                     KPSKADRHLGDGEVLEVGDLKFEVWHTPGHTPGSICLYAAKHSAIFTGDTLLRMSIGR
                     TDGAGGNKTAMLTTIERLSELPDDTRVSPGHGLATTIKAERWLLEIVTAGS"
     gene            complement(1012..1686)
                     /locus_tag="VFH60_05620"
     CDS             complement(1012..1686)
                     /locus_tag="VFH60_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET6313295.1"
                     /translation="MTLGERLKQARLARNLSLTALATATRLSKGFLSQVETGATNPSL
                     ASLQRLSAALSVSLSDLLAADARPSISSAPRPRLVRRVLPERHKSSLTETGRSAERAV
                     YLAHLLPGGYLEAASSPSGEEAFVLVVSGEVHFSQPGTSLRLGEGDSLTFPLAPQYRL
                     VAQGGTVASLLLVVRSAQDLPRLVETPFVEPLPARGRGGVAAAEAHGPLRLVAMRAAR
                     SAGRGR"
     gene            complement(1830..3230)
                     /locus_tag="VFH60_05625"
     CDS             complement(1830..3230)
                     /locus_tag="VFH60_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6313296.1"
                     /translation="MHEKVSPGGTARPGLRLMLALVLAFFLGVTVLALAVPPSAYADE
                     EPPISSELATPSPAPADPIQTTTSDDSSEAPMPTEIPGSTATVSPTSPPPSETRSAPT
                     GEAPAPSRTPLPTSTTTPQPTARGDGWQVNSLRDFAETVGWPPVVLVEANDRLSVRTS
                     SSPDDWGSASIRSFSFPAGSAAAFAAEQEDARLSGYQLSRDTFYTFPAYAGSKTGANG
                     VVVERRYRWLVQSWVLGIDVRRAGVSQADVLDLAQQLLAVATQNGLPVPPGASDMPAP
                     TPQNPAADTPQGCSARFSDVPEGMWAYNYITELACMGVVSGYSDGTFRPQSATTRAQL
                     VKMVVLLKGIPLDAPAVATFDDVSTSHPFYRYVETAVKQGLITGYTDGSFKPDAPVSR
                     AQVAKIVVKALGWSLQVPASPATLCDVPAAHWAYVYVQVAIDHGIFTGYGDGCFHPDD
                     FATRAQLAKVLVLSGR"
     gene            complement(3268..4503)
                     /locus_tag="VFH60_05630"
     CDS             complement(3268..4503)
                     /locus_tag="VFH60_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HET6313297.1"
                     /translation="MRAEIVSIGTELLLGSITDTNASYLAQRLALLGIDCFYISQVGD
                     NPARLQEILARAWGRSDLTVTTGGLGPTVDDLTREAIAEMLGETLYTDKELAEHVRQF
                     FARRGSPMPEANLKQAALIPSGAPIDNPIGTAPGWWVSRQAGGTQRIMVSMPGVPFEM
                     KRMWEIEVEPRLRELSPTRIVSRTLKVLGMGESAVEQVVVDLMSGSNPTLAPYAKQDG
                     IHLRITAKAGTEEAARAMIEGLEEQVRSRLGRAVYGADDESPSGAVLALLERAGVRLA
                     VLEVGEGAIGSVSPQLATSQLVVGSTAARDLGHAAQLLGTPQGAQDDLDLPGLALQAA
                     KNGADVVLALAGRVADSPGRTGSVEAECEVVVVWTGGPSKEPVRASYRWRTARSEVSR
                     LFGLMSLNQLRLVLLEQES"
     gene            complement(4578..>5294)
                     /locus_tag="VFH60_05635"
     CDS             complement(4578..>5294)
                     /locus_tag="VFH60_05635"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6313298.1"
                     /translation="VPDVPQVQEVLFGSDGLSSNARQGLLFIDMSTITPTAARDHYER
                     LKGLGVAAVDAPVSGGPARASDGTLTIMVGGDVEAYERALPVLKALGKNITHVGPPGS
                     GQAVKLVNQLMISIIMVANAEALTLGVKAGVPLATMLDVIGTSSGSNYLMQNWLPKTL
                     FKDDLQSGFALDLLMKDLRAALSWAGELGLPAFGGALAQQLYRLARTQETSRLDYSVV
                     ASVYEEAAGVRLRLSGDEDR"
BASE COUNT          931 a         1726 c         1680 g          957 t
ORIGIN      
        1 agcagatagc gattggctca tcgaggcggc cgcggcggca ggcaggctcg cacggtcggg
       61 cgcacaccct ccccaggatg ccggggatga cgttgtcctc aaggtgtatc tggtaggacg
      121 actcgtagtc ctcggccgcg atgtgggcta tataccgcgc tacgtcggtg taggcggggc
      181 aggcgtactg gcaggagatg ttcgcaagga accattcgtt gtcgcccgtg ttcaactggt
      241 agggcgcggc gttacgctca gcgtgcccta cgctgctctc cgaccgcacc agggccatca
      301 gtcgcatccg ttcatcggcc atgtggtgtt cctccgtcta ggcttcttca gtgttccgaa
      361 tgcgcgggct tgttcagctg cccgccgtta caatctccag tagccagcgc tccgccttta
      421 tcgtcgtcgc aagaccatga ccgggggaga ccctggtgtc gtcgggaagt tccgacagcc
      481 tctcgatagt cgtaagcatg gcggtcttgt tgccacccgc gccgtcggtg cggcctatgc
      541 tcatgcgaag cagggtgtcg cccgtaaaaa tagcgctatg tttggctgcg tacagacaga
      601 tgctgcccgg cgtatgtccc ggtgtgtgcc agacctcaaa cttgaggtcg cccacttcca
      661 gcacctcgcc gtcgcccagg tgccggtcgg ccttgctcgg cttgatcttg gcagccagct
      721 cctcatcgga ggtcaggccg gggtttgcca gccgtgcagc gtcccagaaa tgggcgcaga
      781 gttgcgcgcc ggtggcctcg cacatcgcta cgttatccgc gatgttgtcc cagtggccgt
      841 gcgtgctgac tacaaacggc acctgcaact gctcctcccg gagcacctgc acgattttcc
      901 tcgtgctgcc gagcggcgcg tctatgatga cgccggtgcc gccctgtctg tcgtagacca
      961 ggtaagcggt ggtgttgaaa gggccgcccc cgaatggccg cacgatcatg ttcagcgtcc
     1021 ccgtccggcg ctgcgggccg ccctcatagc caccaggcgc aacggtccat gagcctcggc
     1081 agcagctaca ccgccgcgcc ctcgtgccgg gagcggctct acgaagggcg tttccaccag
     1141 gcgaggcaag tcctgcgccg agcgcaccac caacagtagc gaggcaacag tcccaccttg
     1201 cgccaccaac ctgtactgcg gggccagggg gaacgtcagc gagtcgccct cccccaatct
     1261 gagggacgtt cccggctggc tgaagtggac ctcgccggag accaccagga cgaacgcttc
     1321 ctctccgctc ggagacgacg ctgcttcgag gtacccaccc ggcagcaggt gagccaggta
     1381 gacggcccgc tcggcactcc taccggtctc ggtgaggctt gatttgtggc gctcgggcag
     1441 gacgcgcctc accaatcgtg gcctgggggc ggaggagatg ctgggacggg cgtcggccgc
     1501 caggaggtcg gacagcgaca ccgagagggc ggctgacagc ctctgcaggc tggcgaggga
     1561 gggattggtc gccccggtct cgacctgact gagaaagccc ttgcttagcc gcgtcgccgt
     1621 agcgagggct gtcaggctca ggttgcgggc aagccgcgcc tgcttcagcc tttcaccgag
     1681 cgtcatgagc atctcctgtt tagtacagac aaacccgttt gctgccacta taccaacggc
     1741 tacatgcgtt gtcaagagtg agatggaccg aggcggcagc acgccacatg cgcctgcatg
     1801 cttgcaagtt agccgctgga ccgcccgggc taacggcccg acagaacgag cacttttgcg
     1861 agctgtgcgc gcgtggcgaa gtcatcgggg tggaagcacc cgtcgccgta cccggtaaat
     1921 ataccgtggt ctatcgccac ctggacgtaa acgtaggccc agtgcgcggc cggtacgtcg
     1981 cagagcgtgg cgggtgaggc gggcacttgc aggctccagc ctagcgcctt gactactatc
     2041 ttggcgactt gcgcgcggct gactggggcg tccggtttga acgagccgtc ggtgtatccg
     2101 gtaatcagcc cctgcttgac agccgtttca acgtagcggt agaaggggtg ggaggtgctg
     2161 acatcgtcga acgtggcgac cgcgggcgcg tccaggggga tgcctttgag gagcactacc
     2221 atctttacaa gctgcgcgcg agtggtcgca ctttgcgggc ggaatgtgcc gtcgctgtag
     2281 cctgagacga cccccatgca agccagttcc gtgatgtagt tatacgccca catgccctcc
     2341 ggcacgtcgc tgaagcgggc agaacagcct tgaggcgtgt cagcggcggg gttctgaggg
     2401 gtaggggcag gcatgtcact cgctccgggc ggcaccggga gaccgttctg ggtggctacc
     2461 gccaggagtt gctgtgccag atccagcacg tcggcctgcg acacaccggc cctccgaacg
     2521 tcaatcccca gcacccagga ctgcacgagc catcgatatc gtcgctcaac caccacgccg
     2581 ttcgcacctg tcttggagcc ggcgtaggcg gggaacgtgt agaaggtgtc tcgagaaagc
     2641 tgatagcccg agagacgcgc gtcctcctgc tctgcggcaa acgcggcggc ggaaccggcc
     2701 gggaaactga acgagcgtat ggaggccgac ccccagtcgt caggtgagga gctcgtgcgc
     2761 acgctcagcc ggtcgttagc ttcaactagc accacgggcg gccagcccac cgtctcagcg
     2821 aaatcgcgaa gggagttgac ctgccacccg tcaccgcgcg cggtgggctg tggggtggtg
     2881 gtgctcgtcg gcaatggggt ccgtgaggga gcgggagcct cgccggtggg cgcgctacgc
     2941 gtctcggacg gtgggggact cgtgggggaa accgtagccg tggagccggg gatctcggtc
     3001 ggcatcggag cctcgctgga gtcgtccgag gttgtagtct gaattgggtc ggcgggtgcg
     3061 gggctaggag tagcaagctc cgacgaaata ggaggctcct cgtccgcgta cgcgctgggc
     3121 ggcacggcga gggcgagcac ggtgacaccc aggaagaagg caagcaccag cgcaagcatc
     3181 agacgcaaac caggccgcgc ggtgccgcct ggcgatacct tctcatgcat tttggtgcta
     3241 ctcgctcgaa acatataccg gtcccagcta actttcctgt tcaagcaaca caaggcgcag
     3301 ttgattcagc gacatgaggc cgaagagcct cgacacttcg gaacgcgcgg tccgccaccg
     3361 gtacgaggcc cgtaccggtt ccttgctagg gccgccggtc cagaccacta caacttcaca
     3421 ctcggcttcc acagagccgg tccggccggg gctgtccgcc acgcgcccgg ccagcgcaag
     3481 gactacatcc gcaccgttct tggctgcctg caacgcaagg cccggcagat caagatcatc
     3541 ttgcgcgccc tggggggtac caagcagttg ggccgcgtgt cccaggtctc tggcagcggt
     3601 agagccaacc accagttgcg aggtcgcgag ttgaggcgac acagagccga ttgcgccctc
     3661 tccaacctcc aacacggcga gcctgacacc cgccctttcc agcaatgcaa gcacggcccc
     3721 cgaaggactc tcatcgtccg ccccgtacac cgctctgccg aggcgcgagc gtacctgctc
     3781 ttcaagaccc tctatcatcg ccctggcggc ctcctcggtg ccggccttgg cggtgatacg
     3841 cagatggata ccgtcctgct tcgcgtatgg ggccagcgtg ggattggagc ctgacatcag
     3901 gtccacgacc acttgctcga ctgccgactc gcccattccc agcaccttca acgtccttga
     3961 aacgatgcgg gtagggctaa gctctctcaa cctcggctcc acctcaattt cccacatccg
     4021 cttcatctcg aacggcacgc ccggcatact caccatgatg cgctgcgttc cgcccgcctg
     4081 ccggctcacc caccaccctg gcgccgtgcc aatcgggttg tctataggcg cgcccgatgg
     4141 aatcagcgcc gcctgtttga ggttggcctc cggcatgggg ctgccccgcc gcgcgaaaaa
     4201 ctgcctgacg tgctcggcaa gttccttatc ggtgtagagc gtctcgccca gcatctcagc
     4261 tatggcctcc cgggtgaggt cgtccaccgt cgggcccagg ccgcccgtgg tcactgtgag
     4321 gtcgctacgt ccccaggccc tcgccaggat ttcctggagc cgcgccgggt tgtctcctac
     4381 ctgggaaatg tagaaacaat caatgcccag cagggcaagc ctctgggcaa ggtaggaagc
     4441 gttcgtgtcc gtaatgctgc ctagcagcag ttccgtgccg attgacacga tctcggcccg
     4501 catattacac acctgccctt gtgcaattct gcaagcacgc catgattata gcacacgcgc
     4561 aagcgcccac caatagccta cctgtcctca tcgcccgaaa ggcgcaaccg cacccctgcg
     4621 gcctcttcgt agaccgaggc cacgacggag taatcgaggc gagaggtctc ctgggtccgg
     4681 gccagcctgt atagctgctg agcgagcgcg ccaccgaagg caggcaggcc aagctctcct
     4741 gcccagctta gcgcggcacg caggtctttc atgagcagat cgagcgcgaa accgctttgg
     4801 aggtcgtcct tgaacagggt cttgggcagc cagttctgca tcaagtagtt ggagccgctg
     4861 gaggtaccta tcacgtctag catggtggcg agtggcaccc ccgcctttac gcccagggtg
     4921 agggcttcgg cattggcgac catgatgatc gagatcatca gctggtttac cagcttgacg
     4981 gcttgcccgc tgcctggcgg gccgacgtgg gtaatgttct tacccaaggc cttgagcacc
     5041 ggaagcgccc gctcgtatgc ctccacatcg ccgcccacca tgatagtcag agtaccatcg
     5101 ctcgcccgcg caggcccgcc gctcacgggg gcatccacag ccgcgacgcc cagccccttc
     5161 agcctctcgt aatggtcgcg ggcggccgta ggcgtaatcg tgctcatgtc gatgaaaagg
     5221 aggccctggc gggcgttgct gctcaggcca tcgctgccga aaagtacctc ctggacctgg
     5281 ggcacgtcgg gcac
//
LOCUS       DASRWZ010000363         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_236381, whole genome shotgun sequence.
ACCESSION   DASRWZ010000363 DASRWZ010000000
VERSION     DASRWZ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236381"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1082
                     /locus_tag="VFH60_05640"
     CDS             195..1082
                     /locus_tag="VFH60_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313299.1"
                     /translation="MYQDNQNENQSIIGPNAAGYAPESSSTYAPAPIPSPNGYAPNGR
                     VPAIQAQGLQKAYGQHWAVKNANLLVWPGEIVGFLGPNGAGKTTTIKMLTGLVRPSAG
                     RAAISGFDIQAQPVQAKATFGYVPDTPNLYGKLSGWEFLRFIARLYRVPTEQAERRAN
                     ELLRLFDLYEAASDLAEGYSHGMQQKLALAGALVHDPKVLFLDEPTVGLDPRSARLIK
                     DMLQQLRDRGTAVFFSTHILEIAERMCDRVMIINNGEIIASGTMDELRGRGGTGSLED
                     IFLKLTGGAEYAAIAEVLG"
     gene            1079..>1924
                     /locus_tag="VFH60_05645"
     CDS             1079..>1924
                     /locus_tag="VFH60_05645"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313300.1"
                     /translation="MISDVGAGNSGLRAVIADTWLLLALRWQIAWNSFKGRKTSTQVF
                     SVLGGFVLFLFFASVSAGAGLLAGALLRRFPNAELEPVLPSLLLTGVALFLLIGSFGT
                     ALGSLFLSSDLDLLMTAPVDRKAVFVSKILNGMVWNYMLVFALAVPALITYGLGLGYG
                     PAYYLLALITLLGTPLLPAALGAILVLLVARFAPARRVREVLGLMGALFGISCALIGQ
                     TSRFWLRSFTETGSNDTEQFLGGLRGIIAWPIPPFMAGRGLEAAGRGDIVGALANLSV
                     FLLLTF"
BASE COUNT          335 a          608 c          635 g          346 t
ORIGIN      
        1 ggaggaacat tggtgcaaga gtaggaagag tcaagaacgg gcggctgatc cttcgctacg
       61 ctcaggatga caggggggag tgtggccgcc gcaatcgtgt catggtccca atccgttccg
      121 aagtaaccgg gatgcgtcat aaggcgtaca ataaagagtg gcacacatga ggtccaagag
      181 gaagtaaaca aagcatgtat caagacaacc agaatgagaa tcaaagcatc atagggccga
      241 acgccgcagg ttacgccccc gaaagctcct caacctacgc gcccgcgccc attcccagcc
      301 ccaacggcta cgcgcccaac ggcagggtgc ccgcgataca ggcgcagggc ttgcagaaag
      361 cttacgggca gcactgggcg gtgaagaacg ccaacctgct ggtgtggccg ggggagatcg
      421 tgggcttcct ggggccgaac ggcgcgggca agacgaccac gataaagatg ctcaccggac
      481 tggtcaggcc cagcgcgggc cgcgcggcca tatcgggctt cgatatacag gcgcagccgg
      541 tgcaggccaa ggccaccttc ggctacgtgc ccgacacccc caacctctac ggcaagctga
      601 gcggctggga gttcctgcgc ttcatcgccc gcctgtaccg ggtgcccacc gagcaggcag
      661 agcgccgcgc caacgaactg ctgcgcctgt tcgacctcta cgaagccgcc tccgacctgg
      721 ccgagggcta cagccacggc atgcaacaga agctggcact ggcgggcgcg ctggtgcacg
      781 accccaaggt gctcttcctc gacgagccga ccgtgggcct cgacccccgc tccgcccgcc
      841 tcatcaagga catgctccaa cagttgcgcg accggggcac ggccgtcttc ttctccaccc
      901 acatacttga aatcgcggag cgcatgtgcg accgcgtgat gatcatcaac aacggcgaga
      961 tcatcgcctc cggcacgatg gacgaactca ggggccgggg cggtaccggc tcgctcgaag
     1021 acatcttcct caagctcacc ggcggcgccg agtacgcggc cattgccgag gtattagggt
     1081 gatttcggat gtgggagccg ggaattcggg cttgagggcg gtaatagctg acacgtggct
     1141 gctgctggcg ttgcggtggc agatcgcgtg gaacagtttc aaggggcgaa agaccagcac
     1201 gcaggtcttc tcggtgctgg gcgggtttgt gctgttcctc ttcttcgcct cggtgtcggc
     1261 gggggcgggg ttgctggcgg gggcgttgct gcgccggttc cccaatgcgg agctggagcc
     1321 ggtccttccg agcctgctac taacgggtgt ggccctgttc ctcctgatag gcagcttcgg
     1381 cacggccctc ggctcgctct tcctgtcaag cgacctcgac ctgctcatga cggccccggt
     1441 ggaccgcaag gcggtgttcg tcagcaagat cctcaacggc atggtgtgga actacatgct
     1501 ggtgttcgct ctggccgtgc ccgcgctcat cacgtacggg ctgggtttgg gttacgggcc
     1561 tgcgtactac ctgctggcgc tcattacctt gctgggcacc ccgctcctgc ccgccgcgtt
     1621 gggtgcgata ctggtgctcc tggtggcgcg cttcgccccg gcccgccgcg tgcgcgaggt
     1681 gctgggcctc atgggcgcgc tgttcggcat ctcctgcgcc ctcatcgggc agacctcgcg
     1741 tttctggctg cgctctttca ccgagaccgg cagcaacgac acggagcagt tccttggggg
     1801 tttgcgaggc ataatcgcct ggcctatacc gccgttcatg gcggggcgcg ggcttgaggc
     1861 agccgggcga ggggacatcg tgggggcgct cgccaacctg tcggtgtttc tgctgctaac
     1921 gttc
//
LOCUS       DASRWZ010000364         3519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_442475, whole genome shotgun sequence.
ACCESSION   DASRWZ010000364 DASRWZ010000000
VERSION     DASRWZ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442475"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..2891
                     /locus_tag="VFH60_05650"
     CDS             273..2891
                     /locus_tag="VFH60_05650"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6313301.1"
                     /translation="MLEQVVSVADYQLSLYSIPTLITTAAILLLGLLVLVRERVSLVS
                     LSFALVTFVVSIWLFGFSLMYSAPNPTSALWWNKVAYLGVPFIAPATYQFTVVVLKLY
                     QRFKKVVAISWVLGGVFSALSVWSDLLIDRVQSFWWGYYTHFGPVGAVFILYFVSLLV
                     ASMGHYWVEYRKSESLKHKLRIKWLMIAFAIGYIGAFDFVGAYGIPLYAFGYLAILGF
                     LCVAVYAIGKYHLLDITPAFAANQIINTITDALLVLDHEGIIRVLNRAASNLLGYPAN
                     ALTGKPITTVFDGPQLDEVKSRLASEGSLQDFELSYTGGDGVERFLSLSASTMQAKDD
                     QPVAFVCIVRDVSEKKRSEAAMRESEERYRAVVEQTSEGIFLADLETRRILEANASFL
                     EMLGYSADEVTGLTMYDFIAHDRASIDVNMERLRELKRLAIGERTYRRKDGSLVDVEV
                     STNLIYHAGRHVICVSAHDMTERKRHEERIKRQLERVAALRNIDTAITASLDLRITLS
                     IILDQVTAQLKVDAADVLLLNGRTQMLEYGAGRGFRTGGISQSQLRLGEGYAGKAALQ
                     RHIFRLDELSETGDLLRAPLIAGEDFVTYFAVPLLAKGQIKGVLEIFHRSKLTPDGEW
                     LDFLETLAGQAAIAIDNATLFDELQQSNLELALAYDITLEGWSRALDLRDKETEGHTQ
                     RVTEMTARLARYMGVSDSEMAHVRRGALLHDIGKLGIPDSVLLKPGPLTDEEWGVMRL
                     HPVYAYQWLSSIEYLRPALDIPYCHHEKWDGTGYPRGLKGEQIPRAARIFAIVDVWDA
                     LRSDRPYRPAWSEEKVRDHLRALSGTHFDPEVVAAFLSLETLPMELALTVPHELSMRS
                     TTPSRQSAQLSRSRLG"
     gene            complement(2924..>3519)
                     /locus_tag="VFH60_05655"
     CDS             complement(2924..>3519)
                     /locus_tag="VFH60_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET6313302.1"
                     /translation="EDASMEVKFFETPSELREWFEENHQTEKELWVGFYKKGSGRPSI
                     TWPEAVDQALCFGWIDGIRKSIDADSYMNRLTPRRRGSTWSAVNIKRVGELIELGLMQ
                     PAGLAAFEKRDEKKAQLYSYEQANSKLTEAYEEQFRENERAWSFFQAQAPWYRRSAYW
                     WIMSAKKEETRLKRLAILIEESEKGSKLSTITGGSKR"
BASE COUNT          663 a         1124 c         1065 g          667 t
ORIGIN      
        1 cgcgcgaaga acgtgcccac cggccaacag tctcaacgtc gaagaaggcc gagggtaatg
       61 tggcatgcta ataagagcat gtcgcttttg cttgcatgat gccaggaccc attacagacc
      121 tgcaccatag cctgggccga aacctcaacc aacagaccaa ccagagacca accagcacac
      181 cgcaccagta cagcacacag agagtagcac taaatgaccg tcgtaacccg ccaggggccg
      241 tgcttccaaa catccattag agaagcaggc ccatgctaga acaagtagtc agcgtggccg
      301 attaccagct gagcttatat tcgataccga cgcttatcac gacggctgcc atcctcctgc
      361 tggggctact ggtgctcgtg agagagcgcg tatccctcgt cagcctctcc ttcgcgcttg
      421 tgactttcgt ggtcagcatc tggctgttcg ggttctcctt gatgtactcc gcgcccaacc
      481 ccacgtcggc gctgtggtgg aacaaggtgg cgtacctggg cgtgcccttc atcgcccccg
      541 ccacttacca gttcacggtg gtggtgctca agctctacca gcggttcaag aaggtcgtgg
      601 cgataagctg ggtcctgggc ggggtattct cggccctctc ggtctggtcg gacctcctca
      661 tagaccgcgt gcagagcttc tggtggggat actacacgca tttcggcccg gtgggcgccg
      721 ttttcatcct gtacttcgtg tcgctgctgg ttgccagcat ggggcactac tgggtggagt
      781 accgcaagtc cgaaagcctc aagcacaagc ttcgcatcaa gtggctcatg atcgcgttcg
      841 ccataggcta tatcggcgcg ttcgacttcg taggcgcgta cggtatcccc ctgtacgctt
      901 tcggctacct ggcgatcctg gggttcctgt gcgtagccgt gtacgccatc ggtaagtacc
      961 acctgctcga catcacgccc gcgttcgccg cgaaccagat tatcaacacg atcacggatg
     1021 cgctgctggt cctcgaccat gaaggcataa tccgggtgtt gaaccgcgcc gcgagcaacc
     1081 tgctgggcta cccggcgaat gcccttaccg gcaagcccat cacgacggtc ttcgacgggc
     1141 cgcaattaga tgaggtcaag tcccggcttg caagcgaggg ttcgctacag gacttcgagt
     1201 tgtcctacac gggcggcgac ggggtcgaac gcttcctcag cctctcagcc tccaccatgc
     1261 aagccaagga cgaccagccc gtggcgttcg tgtgcatcgt ccgcgacgtt tccgagaaga
     1321 agcggagcga ggcggcgatg cgggagagcg aagagcggta ccgggcggtg gtggagcaga
     1381 cctcggaggg catattcctg gccgacctgg agacccggcg catcctcgaa gccaacgcct
     1441 cgttcctgga gatgctcggt tactccgccg acgaagttac aggcttgacg atgtacgact
     1501 tcatcgcgca cgaccgggca agcatcgacg tgaacatgga gcggcttcgc gagctaaagc
     1561 ggctggcgat tggcgagaga acgtaccggc gcaaggacgg ctcgctcgtg gacgtggaag
     1621 tgagcaccaa cctcatctat cacgcgggcc ggcacgtgat atgcgtctcg gcgcacgaca
     1681 tgaccgagcg caagcggcac gaggagcgca taaagcggca actggagcgg gtggcggccc
     1741 tgcgcaacat cgacacggcc atcacagcca gcctcgacct gcgcatcacg ctcagcatca
     1801 tcctggacca ggtgacggcg cagttgaagg tggatgcggc ggacgtgctg ctcctgaacg
     1861 gcaggacgca gatgctcgaa tacggggcgg ggcgcggctt ccgcaccggg ggcatcagcc
     1921 agtcgcagct acggctgggc gagggctacg cgggcaaggc ggccctccaa cggcacatct
     1981 tccgcctgga cgagctttcg gagacgggcg acctgctgcg cgccccgctc atagcaggtg
     2041 aggacttcgt tacctatttc gccgtgcccc tgcttgccaa gggccagata aagggtgtgc
     2101 tggaaatctt ccaccgctcg aaattgacac ccgacggcga gtggctggac ttcctggaga
     2161 cgctcgccgg gcaggccgct atcgcgatag acaacgccac gctctttgac gagctacagc
     2221 agtccaacct ggagctggcg ctggcctacg acatcacgct ggaaggctgg tcgagggcgc
     2281 tggacctgcg cgacaaagag accgaggggc acacccagcg ggtgaccgag atgactgccc
     2341 gactggcgcg ctacatgggg gtctctgaca gcgagatggc gcacgttcgc cggggcgcgc
     2401 tgctgcacga catcggcaag ctgggcattc ccgattcggt gctgctcaag cccggccccc
     2461 tcaccgacga ggagtggggg gtcatgcgcc tccaccctgt ttacgcctac cagtggctat
     2521 cctcgattga gtacctgcgc cccgcgttag acattcctta ctgccatcat gagaaatggg
     2581 acggcaccgg ctaccctcgc ggcctcaagg gagagcagat accgagggcc gcacgcatct
     2641 tcgccatcgt ggacgtgtgg gacgcgctca ggtcggaccg accgtaccgg cccgcgtggt
     2701 cggaggagaa ggtccgcgac cacctgcgcg cgctgtcggg cacgcacttc gaccccgagg
     2761 tagtggcggc cttcctgagc ctcgagacgc taccgatgga gctggcgctc acggtgccgc
     2821 atgagctgtc gatgaggtct acgacgcctt cacggcaaag cgcgcagctc tcaaggagcc
     2881 gcctcggttg agaaggggac gccctgctac gatactggtg cccctaccgc ttcgacccgc
     2941 ccgtaatcgt tgacagcttg cttcccttct ccgattcctc gatcaagata gccaggcgct
     3001 tcagccgcgt ctcttccttc ttcgcgctca tgatccacca gtaagcggac cgccggtacc
     3061 acggtgcttg cgcctggaag aagctccacg ctcgctcgtt ctcgcgaaac tgctcctcgt
     3121 aggcctcggt cagcttgctg ttggcctgtt cgtaggaata tagctgcgcc ttcttttcgt
     3181 cgcgcttctc gaacgcggcg aggccggcgg gctgcatcag cccaagctcg attagctcac
     3241 ctacgcgctt gatgtttacg gcgctccagg tgcttccgcg ccggcgtggc gtgagccggt
     3301 tcatgtagct gtcggcatcg atactcttcc gaatgccgtc tatccagccg aagcagagcg
     3361 cctggtccac cgcctcgggc caggtgatgc tcggcctgcc cgaccccttc ttgtagaagc
     3421 caacccacaa ttccttctct gtctggtgat tctcctcgaa ccactcgcga agctcggacg
     3481 gcgtttcaaa gaatttgact tccatgcttg catcctcga
//
LOCUS       DASRWZ010000365         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_374469, whole genome shotgun sequence.
ACCESSION   DASRWZ010000365 DASRWZ010000000
VERSION     DASRWZ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374469"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1040)
                     /locus_tag="VFH60_05660"
     CDS             complement(111..1040)
                     /locus_tag="VFH60_05660"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6313303.1"
                     /translation="MPRLASLLPVLLVLGTLVAILAADPFASKPRRAVGHPDAGPLVP
                     DVTYCTAGGVALTLDLFLAKPSYQGPRPLLVYAHGGGWTEGDKTWVDRIMSSGELALH
                     GYTVAAVNYRLAPWHTWPAQIEDLKCAIRFLRAHAQEYNIDPDRVGVWGESAGGHLAA
                     MLGVTGPGDGFEGGGGYPEQSSRVQAVVDMFGPADLVAMSEEAPQNGLLGYMLLGREP
                     VVTQLRSASPLTYASSDDPPFLLLHGEKDDLVPFSHSTAMKDRLIAAGVPVTLVPVRN
                     GSHVFVPRGGPISPSGQEIKAIVTRFFDLYLNP"
     gene            1577..2191
                     /locus_tag="VFH60_05665"
     CDS             1577..2191
                     /locus_tag="VFH60_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET6313304.1"
                     /translation="MEPHIPALKDHSIAVIVLAAGRGIRMRQASKLLLPLADGKTLLQ
                     HSVENALALRPAETLVVVRPDLPKLEQSLDDLPVRCVANPRYAEGMGTSLAVGVLSLG
                     PEIEATLVVLGDEPLVSGNIFGRLVEAFLRERRPVTIPRYGQHFGPPTIFSRELFPML
                     AKLEGDTGGRQIVAGQPGLACVVPFTAGERPADVDTLEDYQALL"
     gene            2742..>2930
                     /locus_tag="VFH60_05670"
     CDS             2742..>2930
                     /locus_tag="VFH60_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313305.1"
                     /translation="MKLGTKPRHSRLASTALAAALMLTTLPASASSYAAPSQAPAGAQ
                     AALPASLPFAASAFEAVWR"
BASE COUNT          579 a          888 c          935 g          528 t
ORIGIN      
        1 ctactttatc ttggcgcgct cttggcgtcc tttgcgtccc ttcggtttcg cgcagggtat
       61 gcttggcggt tccgttcctc gttggaacta gagtcgaggt gctacgattc tcaggggttc
      121 aggtacaggt cgaagaagcg cgtaactatc gccttgatct cctggccgga ggggcttatc
      181 ggcccgcctc tcggcacgaa aacgtggctg ccgttacgca ccggcaccaa cgttacgggc
      241 acgcccgccg cgataagcct gtccttcatc gcggtcgaat gggagaacgg cacgaggtcg
      301 tctttttcgc cgtgcaagag gaggaacggc gggtcgtcgc tggaggcgta ggtgaggggg
      361 ctggcgctgc gtagctgcgt cacgaccggc tcgcgcccca gcagcatgta gccgagcaag
      421 ccgttctggg gggcctcctc gctcatagcc accaggtcgg cggggccgaa catatccacc
      481 accgcctgca ccctgctaga ctgctcaggg tagcccccac cgccctcgaa accgtcgccg
      541 gggccggtca cgcccagcat ggcagccagg tgccccccgg cgctctcgcc ccacacccca
      601 acgcggtcgg ggtcgatgtt gtactcctgg gcgtgggcgc gcaggaagcg aatggcacac
      661 ttgaggtcct caatttgagc gggccaggtg tgccaggggg ccagcctgta gttgaccgcc
      721 gccaccgtgt agccgtgcag ggcaagctcg ccggagctca tgatgcggtc cacccaggtc
      781 ttgtcgccct ccgtccagcc gccgccgtgc gcgtagacaa gcagcggcct cggcccctgg
      841 tacgaaggct tggccaggaa caggtcaagc gtaagcgcga ccccgcccgc cgtgcaatag
      901 gtaacatcgg gcaccagcgg ccccgcatcg gggtggccta cagcgcgccg gggcttgctc
      961 gcgaacgggt ccgctgccag tatcgccacc agcgtgccga gcacaagcaa caccggcagc
     1021 aggctcgcca ggcgtggcat gcgcgggtgt tgggcctgga agtggaaggg agaccgagag
     1081 cgcccctgcg cagatcgaaa ggcgcgggga gccacccggc gggctgtact gcctgcggat
     1141 ttagatgaac gagttgagcg cggacgcgtg tcagtcatga gcgcaaggcg ggctataaag
     1201 ggtgacaatc aatgcaggcc cagtaactga gtatatgagg tttcgcccca actgggcaag
     1261 tgtgtagcac aagtgcgacc tgttgaaggt gggcacccaa aggtggccct gtcatcctga
     1321 acgcagtgaa ggatctaaag aaggtagtag cgggatacag ttgaacgtag aggcaacagt
     1381 gctgaatgtt cagcgcaggc acaaaggcaa catcttagat ccttcactgc gttcaggatg
     1441 acagggccac ccttgggcgg tgtctcccca caactggacc ccatttcacg ttgcagcggt
     1501 ttgcctaacg ctataagata gagcgggaca aatgggaatg aattaaagaa tgtcgctgca
     1561 taagaacgcg agagatatgg aacctcacat acccgccctc aaagaccaca gcatcgccgt
     1621 aatagtgctt gcagcagggc gaggcattcg catgcgccag gcctccaagc tgctgctgcc
     1681 cctggccgat ggcaaaacgc tgcttcagca ctctgtcgag aacgcgctgg ccctgcgacc
     1741 cgccgaaacg ctggtcgtgg tgcggcccga cctgcccaaa ctggagcaga gcctggacga
     1801 cttaccggtg cgttgcgtag ccaatccgcg ctacgcggag gggatgggta cgtccctcgc
     1861 ggtgggcgtc ctgtcgctgg ggccggagat agaggcgaca ttggtggtgc tgggcgacga
     1921 gcctctggtg agcgggaaca tcttcgggcg gctggtggag gcgttcttgc gagagcgcag
     1981 gccggtgacc ataccccgct acgggcagca cttcggcccg ccgaccatct tctcccgcga
     2041 gcttttcccc atgctggcga agctggaggg cgacaccggc ggcaggcaga tagtggccgg
     2101 gcagccgggg cttgcctgcg tggtgccttt cacggccgga gaacgccccg ccgacgttga
     2161 cacgcttgag gactaccagg cattgttgta ggaattcgat attgactttc gggcgcgtag
     2221 gataccgtgc tataatggga taggacaatt cgggaccaac aggaggatct gcaacatggg
     2281 cgacaagagt ccgaaaaagg ctgagaagaa gaagaagaag aaggaagtct caaagccgac
     2341 aaactaagag ggacatgcct gccgtgcctt atctgtcagg ataggcgcgt gcgcgcgtgc
     2401 tgagctgatg cggtgctaac aagacgccgc gaagcataat atcaagagcg cagtaccggc
     2461 ggcaggtgct gcgctcttga tattgatagc ccgacttcta agcttcgcct gcctggtgcc
     2521 cgcacagccg gtgagcgcgc cgccgtcacg gacgtgcttg agtgctacca ggccccctat
     2581 aggacttttg taatgattat gtaaggttac tgcttgcgtc ggcctcacta ctaatggtaa
     2641 gataaagcca ggttactcgt tatcacgccc gtaatcatgc tcgtacgatt agaggtacgt
     2701 tctagaccca tgcacatacc aaggagatag gaaccgccca catgaaactc ggtacgaaac
     2761 cacggcattc caggcttgcc tccaccgccc tcgcggcggc ccttatgctc accaccctcc
     2821 cggcttcggc ttcctcgtat gccgcgccgt cgcaggcacc cgccggtgcc caggctgccc
     2881 tccccgcctc cctgccgttc gccgcctcgg cgttcgaggc ggtctggagg
//
LOCUS       DASRWZ010000366         6595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_442487, whole genome shotgun sequence.
ACCESSION   DASRWZ010000366 DASRWZ010000000
VERSION     DASRWZ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442487"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VFH60_05675"
     CDS             complement(<1..750)
                     /locus_tag="VFH60_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313306.1"
                     /translation="MKKLSSILVLVLLVGTCGATLSRAAANRLGLTSWPGVWLADSAF
                     FDVWARADGPVAMQAVGRSWLWGPVPFAVANEAYAESPTGKRLVEYLDKGRMEANDPG
                     GDRTSNWFVTSGLLVSEMVSGRVQTGDTSFETRTPAQVPVAGDATSPDAPPYAAFATL
                     TAPVPRATGNLAQQAVSKGGAVRPLGEPIPGASRSASTPGAYDEISGHNVPAVFTEWM
                     RQTGAVLQGGRLAQGRLMDPLFVLGRPITEAY"
     gene            1021..1314
                     /locus_tag="VFH60_05680"
     CDS             1021..1314
                     /locus_tag="VFH60_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313307.1"
                     /translation="MMDYSGTTRVDYEDILRAVGYFIDQNNLKEVCIIELNEGVLVRG
                     IRYTTDPKAGYQTISESFLFTNEDLEKLLEDAYKRRQPGKGGIFRLRPVRENE"
     gene            1377..2102
                     /locus_tag="VFH60_05685"
     CDS             1377..2102
                     /locus_tag="VFH60_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6313308.1"
                     /translation="MYKGLTVSVVIPCFNEEHGIAAVLQSMPDYVDQVVVVDNNSTDE
                     TGRIARELGAEVIFETRKGYGSAYKAGFPRATGDIVATADGDGTYPTNSIGPIIDYML
                     EKKLDFVSASRFPLVDPKAMRFRNVLGNKILTYTTRVLWLRWIADSQSGMWVMTREAL
                     KKLRLESDGMPFSEEIKLEAIENRSIRFGEYHIHYSERIGETKLFPMKDGIANLLYLF
                     RRRIRRTRLEKLVGLDKGRSEAP"
     gene            2135..3442
                     /gene="xseA"
                     /locus_tag="VFH60_05690"
     CDS             2135..3442
                     /gene="xseA"
                     /locus_tag="VFH60_05690"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET6313309.1"
                     /translation="MPASKSRVIEAGSTALRIFRVGELASYLKHLLEGDEALFDCWIA
                     GEVSNVSQTGSGHFFFTVKERDAQMRCVLFRQQAIWQTVLPSNGTAVLAHGYVSMYEA
                     TGNLQFYVDTLQEEGQGALFLAFEQLKAALEAQGLFAEERKRPIPLMPRRIGLVTSPQ
                     AAALQDMLNVLGRRWPIAEVVLSPTQVQGDGAARQVAGAIAALNALEAEEAVDLIIVA
                     RGGGSIEELWAFNEEVVARAVFASTVPVISGVGHETDTTIIDYVADLRAPTPSAAAEL
                     ATPDIFDLQATIDAWRRMMLNDMRGRIAVAWDEVESERDRLDHLSPMWQVSNDRKQIE
                     TMRKSMDTHLRHRLTLERERIVSAKQRLDALSPLQILKRGYAIVRDLDSGAVVSSIAD
                     AQADHLLEIRVGDGAFGARVVRNRAAESRELWDGDDEDYGEGN"
     gene            3446..3946
                     /gene="xseB"
                     /locus_tag="VFH60_05695"
     CDS             3446..3946
                     /gene="xseB"
                     /locus_tag="VFH60_05695"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET6313310.1"
                     /translation="MAQERKNGSGLAHGSNSATFEQQFGRLQEVVQRLSDGNLTLQEA
                     LSSFEEGMALADNCSRMLEEAELRVQQVSERAARGGSVAAAELDLAMRARPADEEPEL
                     LTFEIETYETTVLIEEPVSKGREAAPPVSSAKSNAPQKQPPAPAPAPAPAAPARPLDP
                     LFDEDD"
     gene            3950..4753
                     /gene="trmD"
                     /locus_tag="VFH60_05700"
     CDS             3950..4753
                     /gene="trmD"
                     /locus_tag="VFH60_05700"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET6313311.1"
                     /translation="MLLDILSLFPEMFPGVLGASIFKRGMEAGHMRVRLHNIRHYATD
                     KHHTVDDYPYGGGAGMVMKCEPLFRATEWVYHLPDAPPGISLYEPPPDALIGPPEPIP
                     APAGTPIILMSPQGRVFTHANAQELARHPRLIILCGHYEGVDERVREHLVTDEISIGD
                     YVLTGGELAAMVVADAVARQIPGVLAEGSAHDESHARGLLEYPQYTRPADFRGWTIPP
                     TLVSGHHAQVEAWRRREALRATLLRRPDLLEHAELTPQERKWLEEMTNS"
     gene            complement(4765..6459)
                     /locus_tag="VFH60_05705"
     CDS             complement(4765..6459)
                     /locus_tag="VFH60_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313312.1"
                     /translation="MVAFTAATFMVLAVANASSAAPDGFADKAFLDIWQRTDAPVLDG
                     KAQRSWYWGPQPGRALEEPYNGVEGGTRLVQYFDKARMEINNPGGDRKSEWFVTTGLL
                     VAEMVSGKQQVGDKQFVPKQAAEIAVAGDGLTDDPDAPTYTSFRLVASLGGPGNNRAP
                     NMVGKTVTSTINRAGQVGSNTGLGLYPGAKLAAYSDVLGHNIPQAMWDFLNLKGTVQQ
                     DGKLSANQTLANWVFVMGYPISEPYWTRVKIGGVFNDALVQLYERRTLVYVPALDKGW
                     QVQMGNVGQHYHRWLYGGPLPAPLLAHASPTQAPPAIPDPIDATIEPNSAPAGTPLEV
                     SLSDFQPGEEIESWFTAPDGSARDAGINLKAGPDGRVDTVTVPTDGFTTGLWAITFHG
                     KASNHESVAYFYLSPADPASTPRPTRPPATRTPTSAATSAATSTRPAAPTRTPGPRGS
                     VTPTATYPAVPTEPSTGLLLSVRPGSGSPDTEFTFSATNLTPNEPVTIKFTDPTGATV
                     FPANSNNGQYQANSAGQLSIVLVPSQAFPSAPLGVWYFEVIGQQSKLEGLIGFVLR"
BASE COUNT         1226 a         2123 c         2156 g         1090 t
ORIGIN      
        1 gtacgcctcg gtgatcggcc tgcccaatac gaagagcggg tccatgaggc gtccctgcgc
       61 cagccgccca ccctgtagca cagcccccgt ctgccgcatc cactccgtaa agactgccgg
      121 tacgttgtgg cccgatatct cgtcgtaagc acccggcgtg gaggcgctgc ggctggcgcc
      181 ggggataggc tcacccagag gccggacggc accgcctttg gagactgctt gctgcgccag
      241 gttgccggta gcgcggggga caggagcggt cagggtggca aacgccgcgt atggtggagc
      301 atcagggctt gtggcatccc cggcgaccgg cacctgggcg ggcgttctcg tctcgaacga
      361 agtgtcgcct gtctgcacgc ggcccgacac catctcggag acgagcaggc cgctggtcac
      421 gaaccagttg gaggtgcggt cgccccccgg gtcgttcgcc tccatgcgcc ccttgtccag
      481 gtactcgacc aggcgcttgc ccgtgggcga ctcggcataa gcctcatttg ccaccgcgaa
      541 cggcacaggc ccccacagcc acgacctgcc caccgcctgc atcgccaccg gcccgtccgc
      601 gcgcgcccac acgtcgaaga acgcgctgtc ggccagccac accccaggcc aggaagtcag
      661 ccccaaacgg ttagcggctg cccgcgagag ggtcgcgccg caggtaccca ccagcagcac
      721 caagactaag atagaggata gtttcttcat ctgtaaatcg taacacgtaa cgagtaacac
      781 gccctataca acaaagcggc gcaggcgaaa cgaagttcgg tgaacctagt tacgtgttac
      841 tcgttacgtg ttacgcagca ctcctcacgt aattcgtaat tccctaatga tatcactttt
      901 gcaccgccaa aacccgtgtg ctataatgcc cattgccacg ccggaccgtc atggagctat
      961 gttccagcat tgactgcgct ttcacctggc cggtcgcggt cccgcttgca ggagcatcag
     1021 atgatggact attcgggcac gacccgcgtg gactacgagg atatactgcg cgccgtcggt
     1081 tacttcatcg accagaacaa cctcaaggaa gtctgcatca tcgaactgaa cgagggcgtg
     1141 ctggtgcgcg gcatccgcta caccaccgac cccaaagccg ggtaccagac tatctccgag
     1201 tcgttcctgt tcaccaacga agaccttgag aaactgctgg aagacgccta caagcgccgc
     1261 caaccgggca agggcggcat ctttcgtctc aggcccgtgc gagagaatga atagggtgcg
     1321 attgcgcgag ctttgcagtg tgaggagtgg cgtgaagctg acagggtgct gcttgcatgt
     1381 ataaaggcct gacggtatcg gtggtcatcc cgtgtttcaa cgaggagcac ggcatagccg
     1441 ccgtcttaca atcgatgccg gactacgtcg atcaggtcgt ggtcgttgac aacaactcca
     1501 ccgacgaaac gggccgcatc gcacgcgagc ttggggccga ggtcattttc gagacgcgca
     1561 agggctacgg cagcgcctac aaggccgggt tccccagggc caccggcgac atcgttgcca
     1621 ccgccgacgg cgacggcacc taccctacca actccatagg ccccatcatc gactatatgc
     1681 tggaaaagaa gctcgacttc gtgtcggcca gccgtttccc cctggtggac cccaaggcga
     1741 tgcgcttccg caacgtgctc ggcaataaaa tactcaccta caccacccgc gtgctctggc
     1801 tcaggtggat tgccgactcg cagtcgggta tgtgggtgat gacgcgggag gccctcaaga
     1861 agctgcggct ggaaagcgac ggcatgccct tctcagagga aatcaagctg gaagcgatag
     1921 agaacaggtc gatccgcttc ggtgagtacc acatacatta cagcgagcgc ataggcgaga
     1981 ccaagctctt cccgatgaaa gacggcatcg ccaacctgct ctacctgttc cgccgccgca
     2041 ttcggcgcac ccggctcgaa aagctcgtgg gcctcgataa gggtcgtagc gaagccccct
     2101 agacctaccg caatatcctg tacaggccac tcttatgccc gcatcaaagt cccgcgtcat
     2161 agaagcaggc agcacggccc tccgcatctt ccgggtgggc gagcttgcaa gctacctcaa
     2221 gcacctcctg gagggcgacg aggcgctgtt cgattgctgg atagcgggcg aggtgagcaa
     2281 cgtctcccag accggctcag gccacttctt cttcaccgtg aaggagcggg acgcgcagat
     2341 gcgctgtgtg ctgttccgcc agcaggctat atggcagacg gtgctcccca gcaacggcac
     2401 ggcggtgctc gcccacggct acgtctcgat gtacgaggcc acgggcaacc tgcaattcta
     2461 cgtggacacc ttgcaggagg agggccaggg cgcgctcttc ctcgccttcg agcagctaaa
     2521 ggcagcgctg gaggcccagg ggctgttcgc ggaagaacgc aagcgcccca tcccgctcat
     2581 gccccggcgc atcggcctcg tgacctcacc ccaggccgcc gcgctccagg acatgctcaa
     2641 cgtgctcggc aggcgctggc ccatagccga ggtggtgctc tcgcccacgc aggtgcaggg
     2701 cgacggggcg gcccgccagg tagcgggggc catcgccgcg ctcaacgcgt tggaggcaga
     2761 ggaggcggtg gacctcatca tcgtggcgcg gggcggcggc tcgatagagg aactttgggc
     2821 cttcaacgag gaagtggtgg cgcgggccgt cttcgcaagc acggtgccgg taatcagcgg
     2881 cgtggggcac gagaccgata cgaccatcat cgactacgtg gccgacctac gcgcccccac
     2941 cccctccgcc gccgccgagc ttgccacgcc cgacatattc gacctgcaag ccacaatcga
     3001 tgcctggcgg cgcatgatgc taaacgacat gcggggccga atagcggtgg cctgggatga
     3061 ggtggagtcg gagcgcgaca ggctggacca cctgtcgccc atgtggcagg tcagcaacga
     3121 ccgcaagcag atagagacga tgcgcaagtc tatggacacg catctccgac accgtctgac
     3181 gctggaacgg gagcgcattg tgagcgcgaa gcagcgcctc gacgccctgt cgcccttgca
     3241 gatactcaag cgcggttacg ccatcgtgcg cgacctcgac tccggggcgg tggtcagcag
     3301 catcgcggac gcgcaggccg accacctgct tgagatcagg gtaggcgacg gtgcgttcgg
     3361 tgcccgtgtg gtgcgcaacc gcgccgccga gagccgcgaa ttgtgggacg gggacgacga
     3421 ggactacgga gaggggaatt aggggatggc acaggagcgc aagaacgggt cggggctggc
     3481 ccacgggagc aacagcgcca cgtttgaaca gcaattcggc aggctgcaag aggtagtgca
     3541 acgcctgagc gacggcaacc tcacgctcca ggaggcgctt tcgtccttcg aggagggcat
     3601 ggcgctggcc gacaattgca gccggatgct ggaagaggcc gagcttcgcg tgcaacaggt
     3661 cagcgagcgc gcggccaggg gaggcagcgt ggcggcggct gaactcgacc tcgcgatgag
     3721 ggccaggccc gccgacgagg agccggaact gctgacgttt gagatcgaga cttacgagac
     3781 cacggtgctg atcgaggaac cggtaagtaa gggcagggaa gccgcaccgc ccgtgtcttc
     3841 ggccaagagc aacgccccgc agaagcagcc ccctgctccc gcacctgctc ctgctcccgc
     3901 tgccccggcc cggcccctcg accccctgtt cgacgaggac gactgagcct tgctgctgga
     3961 catcctgtcg ctcttccctg agatgttccc cggcgtgctg ggggccagca tcttcaagcg
     4021 cggcatggag gcggggcaca tgcgggtgcg cctgcacaac atccggcact acgccaccga
     4081 taagcaccac acggtggacg actaccccta cggcggcggc gcgggcatgg tgatgaagtg
     4141 cgagccgctc ttccgcgcca ccgagtgggt ctatcacctg ccggacgccc cgccgggcat
     4201 cagcctctac gagcctcccc cggacgctct gataggcccg cctgagccta tccccgcccc
     4261 cgccggtacg cctatcatcc tgatgagtcc ccagggccgc gtcttcacac acgcaaatgc
     4321 ccaggaactc gcccggcacc cgcgccttat catcctgtgc gggcactacg agggggtgga
     4381 cgagcgcgtg cgggagcacc ttgtcaccga cgagatcagc ataggcgact acgtgctcac
     4441 cggcggggag ctggcagcga tggtggtggc cgacgcggta gcccgccaga taccgggcgt
     4501 actggccgaa ggctccgccc acgacgagtc ccacgcgagg ggcttgctcg aataccccca
     4561 gtacacccgc cctgccgact tccggggctg gaccatcccc cctaccctcg tcagcggcca
     4621 tcacgcccag gtcgaagcct ggcggcggcg cgaggcgttg cgcgcgaccc tcctccgccg
     4681 ccccgacctg ctggaacacg cggaacttac cccccaggag cggaagtggc tggaggagat
     4741 gacgaattct taattcttaa ttggctagcg tagcacgaaa cctatcaggc cctctagctt
     4801 agattgctgg ccgatcactt caaagtacca cacacccagt ggcgcggaag ggaacgcctg
     4861 cgaaggcacc agcactatgc tgagttggcc tgcggagttg gcctggtact ggccgttgtt
     4921 cgagttggcg gggaagaccg tcgcgcccgt ggggtcggtg aatttgatgg tgaccggctc
     4981 gttcggggta aggttggtgg cggagaaggt gaactctgtg tcgggggagc cggagcctgg
     5041 gcgcaccgat agcaacagcc ccgttgacgg ctcggtgggc acggcggggt aggtagcggt
     5101 gggagttacg ctgccacggg gtccgggcgt gcgcgtgggg gctgccgggc gagtagaggt
     5161 ggcggcggag gtcgcggcgc tggtgggggt gcgggttgcg ggcgggcgag taggacgtgg
     5221 ggtgctggcg gggtcggcgg gagagaggta gaagtaggct accgactcgt ggttgctggc
     5281 tttgccgtgg aaggtaatcg cccacaggcc ggtcgtgaag ccgtcagtgg gcaccgtcac
     5341 ggtatcgacc ctgccgtccg gccccgcctt caggttgatg cccgcatcgc gggcgctgcc
     5401 gtcgggtgct gtgaaccagg actctatctc ctcaccgggc tggaagtcgg acagcgacac
     5461 ctccaatgga gtgcctgcgg gcgcggaatt gggttcgatg gtagcgtcaa tcggatcggg
     5521 gatggcgggc ggtgcctgcg tgggcgaggc atgggcgagg aggggcgcgg gcagggggcc
     5581 gccgtagagc cagcggtggt agtgctgtcc gacgttgccc atctgcacct gccagccttt
     5641 gtcgagcgcg ggcacatata ccagcgtgcg ccgctcgtaa agctgcacca gcgcatcgtt
     5701 gaagacgccc ccgatcttga cccgcgtcca gtaaggttcg ctgatggggt agcccatcac
     5761 gaacacccag ttggcgaggg tctggttggc cgagagcttg ccgtcctgct gcacggtgcc
     5821 cttcaggttg aggaagtccc acatcgcctg cgggatgttg tgtccgagca cgtcgctgta
     5881 tgccgccagc ttcgcgccgg gatacagccc caggcccgta ttgctgccca cctgtccggc
     5941 ccggttgata gtagacgtta cggtcttacc caccatgttg ggggcgcgat tgttacccgg
     6001 cccgccgagc gaggccacaa ggcgaaacga ggtgtaggtg ggagcatcgg ggtcgtcggt
     6061 gagaccgtcc cccgccaccg cgatttcggc ggcctgcttt ggcacgaact gtttgtcgcc
     6121 cacctgctgt ttgccggaga ccatttcggc caccagcagc cccgtggtga cgaaccactc
     6181 gctcttacgg tcgccgccgg gattgttgat ctccatgcgg gccttgtcga aatactgcac
     6241 cagccgggtg ccgccctcca ccccgttgta cggctcctct agcgcccgcc ccggctgcgg
     6301 cccccagtac cagctacgct gcgccttgcc gtccagcacg ggggcgtcgg tgcgctgcca
     6361 gatgtcgagg aacgccttgt ccgcgaaccc gtcaggggcg gcactcgatg cgttcgcaac
     6421 cgcaagcacc atgaaggtcg cggctgtaaa agccaccacg aacagcagac gccaagcccc
     6481 gtggcgtcta gagaaacgag cggcaggggt gtccacgctc ggagtttagc acgcgtgcct
     6541 tctgcctgtc aacgcgagta atagacggaa ccgccaagac gccaaggacg ccaag
//
LOCUS       DASRWZ010000367         4458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1127806, whole genome shotgun sequence.
ACCESSION   DASRWZ010000367 DASRWZ010000000
VERSION     DASRWZ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127806"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1375
                     /locus_tag="VFH60_05710"
     CDS             143..1375
                     /locus_tag="VFH60_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HET6313313.1"
                     /translation="MAADFADKQAFLKRIAGAARLRQGPRGVESVLRAIFDTQHDPTT
                     ALTDRLLARVSRMPVPVISAIHQQLEQAGVLEPGSELRLTSDATKVLTERWGWHLSPA
                     VEAASCVACDGTGVAPTGVRWDGVLATLRTHTKAVPEGGGRRATPETNLRRTAYMHGL
                     AALSGKDVLVIGEDVSVSAAIALAGKALSTTGRLARRVVALHTSERMLTGLRDIAVAE
                     GAIIGLVTHNLYHPLPKDLQGEFDTVIVDPPYTLRHLALYVSRAVEACKEEKGRIVLS
                     YRSLDPDERLGVQRTLLDMSLSVEQLTPGFNRYADGALTDLYYIRLTEESAPLIDGEY
                     TEPVHPAQEHPVLNTYVCSSCDSQVTLVSDDKADIWAEQKVLNKAGCPTCGGSTMEPI
                     YTRAFGKNGKGTLGGGKG"
     gene            complement(1380..1943)
                     /gene="cas4"
                     /locus_tag="VFH60_05715"
     CDS             complement(1380..1943)
                     /gene="cas4"
                     /locus_tag="VFH60_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HET6313314.1"
                     /translation="MPGWETSCLGGGVLLRGLAVLLLVGSARLRGRSGLPGGRILLSD
                     TSEERRGRPLYSETYGLAGTPDYLVQTSRGPVPVEVKPGRTDAEPHESHLLQVLAYCL
                     LVEETEGKRPPYGLLRYSSDTFKVDYNNQTRAYIINVIEEMRAASEQADVHRSHEAPG
                     RCRACAYKQICEESLWPTPADENSSSP"
     gene            complement(1958..3187)
                     /locus_tag="VFH60_05720"
     CDS             complement(1958..3187)
                     /locus_tag="VFH60_05720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313315.1"
                     /translation="MRIAVWHNLPGGGGKRALYYHVRGLVERGHTVEAWCPPTADRSF
                     LPLAQLVPERVVPLTWEPKRRRGPVTRAWANYKNVVDKLAALDRHCRQCAGEIQRGGF
                     DILFANGDRDFAVSPIGRHVRLPSALYLQEPYRPLYEAMPQLPWLALPGPRRFSPAYL
                     IRFLHNLIKVQGARAQAREEVLNAQAFDTILVNSHFSRESILRAYGLDATVCYLGVDL
                     DLFRRHDVQREGFVLGLGSFTPAKGVRIVIEAINRMEKPRPRLVWVGNASQPDFLEEM
                     KRLASALEVDLRPRLQVTDEELVGLLNSAGVLAYAPRLEPFGFAPIEANACGLPAVVV
                     PEGGVRETVIDGVNGLWADHDPASLASALHRLLSDGDYAARLGEQGRQMAREKWSLDG
                     AIDRLERRLLETVDKNR"
     gene            complement(3184..4347)
                     /locus_tag="VFH60_05725"
     CDS             complement(3184..4347)
                     /locus_tag="VFH60_05725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET6313316.1"
                     /translation="MSVIIPAYNQGPMLVRAIESALSQTYHNVEIIVVNDGSPDPITR
                     QTATQFEGRIRYIERENGGVAAARNTGIEAARGELIALLDQDDVWLPHRLDVQVRALR
                     AHEHVALVHSSYYLIDEAGHRTGVACLPEREWYALPGLLLDIPIASCTCLIPRWALHE
                     VGLFDPALAGSDDWDLELRMAASGYRFYCVGEPLAEYRLHAANTSRDVNLMARAGLGV
                     LDKFYARSDLPAKARDYKDRAYFNRYAWAASISYGAGKQAEARDYMLKAAHHYPQGVA
                     KGRFLQSLVYAKSDQPSRATAQAAVRFATRTLDPALLPAGVRRKLRSRARLVLALHAT
                     NSRFEMVKGLARALMSDPVLALDREIWSAALRLARRARSKYVMRSAAGRRHAA"
BASE COUNT          801 a         1393 c         1423 g          841 t
ORIGIN      
        1 aagggtgcat ttgcccgctc aatctgccct ggtgctcgaa gcgatacagg gaggctcgta
       61 taacctttag gggtcagggt taggtgttgg gggctgagga aaatagcagc agactctgac
      121 ccctaacccg tggaggcatg atatggcagc agattttgcg gacaagcaag ccttcctgaa
      181 acgcatcgcc ggggccgctc gactcaggca ggggccgcgc ggagtggaga gcgtgctgcg
      241 cgccatcttc gacacgcagc atgacccgac taccgctctc accgaccgcc tacttgcacg
      301 cgtcagccgc atgccggtgc ccgtcatctc ggccatccac cagcagcttg agcaggcagg
      361 cgtactcgaa cctggctcgg agctgcgcct gaccagcgat gcgactaaag tgctcacaga
      421 gaggtggggc tggcatttgt cgcctgcggt ggaggccgcg tcgtgcgtgg cctgcgatgg
      481 tacaggcgtt gccccaacag gtgtgcgatg ggatggggtg ctcgccactc tacgaacgca
      541 tactaaagcc gtgcctgaag gaggcggcag acgggccacg cctgagacca acctgcgccg
      601 cacagcctat atgcatggtt tggcggcact ctccggcaag gacgtgctgg tgatagggga
      661 ggatgtatcc gtgtcggcgg ccattgcgct ggcggggaag gctttgtcta ctacgggcag
      721 gctggcccgc agggtagtgg ccctgcacac cagcgagcgc atgctgaccg ggctgcgaga
      781 tatagccgtt gccgaggggg cgatcatcgg cctcgtgacg cacaacctct accaccctct
      841 gccgaaggac ttgcaagggg agttcgacac cgtgatcgtt gacccgccct acactctccg
      901 gcacctcgcg ctctacgtat cccgcgctgt tgaggcttgc aaggaggaga aggggcgtat
      961 agtcctcagc taccgatctc tcgaccctga tgagcggctg ggtgtgcaac gcacgctact
     1021 ggatatgtct ctatcggtgg agcagctaac gcccggcttc aacaggtacg cggacggcgc
     1081 tctcaccgac ctctactaca tcaggctcac ggaagagtcc gcgcccctga ttgatggcga
     1141 atacaccgag ccggtccacc ccgcgcagga gcaccctgta ctcaatacct atgtgtgtag
     1201 ctcttgcgac agccaggtga cgctcgtcag cgacgacaaa gcagacatct gggccgagca
     1261 gaaggtgttg aacaaggcgg gctgtcccac ctgtggcggc agcacgatgg agcctatata
     1321 cacccgcgcc tttggtaaga acggcaaagg tacgctcggt gggggtaagg ggtaaggggt
     1381 cagggcgagc tgctattttc atcagccggg gtgggccaca gcgattcctc gcatatttgc
     1441 ttgtacgcgc atgcccggca ccgccccggc gcttcatggc tgcggtgtac atcggcctgc
     1501 tctgatgccg cccgcatctc ttctatcacg ttgatgatgt aagcacgcgt ctggttgttg
     1561 tagtctacct tgaacgtatc gctgctgtac ctgagcaggc cgtagggtgg ccgcttccct
     1621 tccgtttcct ccaccagcag gcagtaagcg agcacctgca agaggtggga ctcgtgcggc
     1681 tcggcatcgg tgcgccccgg cttcacctct acaggcacag ggcctcgtga ggtctgcacc
     1741 aggtagtcag gagtgcccgc aagcccatac gtctccgagt agagtggcct gccccgacgc
     1801 tcctctgacg tatccgagag tagtattcgc ccgccgggca aaccgctcct gccccggagg
     1861 cgtgccgagc ctaccagcag caataccgcc agcccccgca gcaacacccc cccacccagg
     1921 caactcgttt cccatcccgg catgggccag gggatagtca tctgtttttg tctacggttt
     1981 cgagcaatct gcgctcaagg cggtctatcg cgccatccag cgaccatttc tcacgcgcca
     2041 tttgcctgcc ttgctctccg agtcgcgccg cgtagtcgcc atcgctgagg agcctgtgca
     2101 gcgcgcttgc cagggatgcc gggtcgtggt cggcccatag gccgttgacg ccatcaatta
     2161 cggtctcgcg gacgccgccc tccggcacta ccacagccgg tagcccgcag gcgttagcct
     2221 cgatgggcgc gaatccgaac ggttccaggc gcggggcgta ggcgagcaca ccggcgctat
     2281 tgagcaggcc gacaagctcc tcgtcggtga cctgcaaacg gggccgcaag tccacttcca
     2341 gagcgcttgc caggcgcttc atctcttcga ggaagtcagg ctgggatgcg ttgccgaccc
     2401 acaccaggcg cggcctcggc ttctccatgc ggttgatggc ctctataacg attcgtacac
     2461 ctttggcggg cgtaaagctc cccaggccga gcacgaaccc ctcgcgttgt acgtcgtgtc
     2521 tgcggaacaa atctaggtcc acgcccaggt agcaaactgt cgcgtccagg ccataggcgc
     2581 ggaggatgct ttcacggctg aagtgcgagt tcaccaggat ggtatcgaac gcctgggcgt
     2641 tgagcacttc ttcacgcgcc tgggcacgcg cgccctgcac tttgatgagg ttgtgtagaa
     2701 accttataag ataggcgggc gagaatcgcc tggggccggg gagggcgagc caggggagtt
     2761 ggggcatcgc ttcgtaaagg gggcggtacg gttcctgtag ataaagcgca ctaggcagcc
     2821 gcacgtgcct gccaatgggg ctgaccgcaa agtcgcggtc gccgttcgcg aagagaatgt
     2881 cgaagccgcc acgctgaatc tccccagcgc attggcggca gtggcggtcc agggcggcga
     2941 gcttgtctac tacgttcttg tagttggccc aggcgcgcgt gacgggtcca cgcctgcgtt
     3001 ttggctccca ggtgagcggt acgacgcgct caggcaccag ttgggcgagg gggaggaacg
     3061 acctgtcggc tgtgggtggg caccatgctt ctacggtgtg tccgcgctcc accaggccgc
     3121 gcacgtggta atagagcgcc cgcttgccgc ccccgccggg tagattgtgc cagacggcga
     3181 ttctcatgcc gcgtggcgtc ttccggcggc gcttcgcatc acgtacttcg agcgtgcgcg
     3241 ccgggccagc ctcagcgccg ccgaccagat ttcacggtct agcgccagca cagggtcgga
     3301 catgagtgcg cgggcgagac ccttcaccat ctcaaaccga ctgttcgtcg cgtgcaacgc
     3361 caggaccagc ctcgctctag aacggagctt gcggcgcacg cctgccggga gaagagcagg
     3421 gtctagcgtg cgcgtggcaa acctgactgc cgcctgtgcg gttgcgcggg aaggttggtc
     3481 gctcttcgcg tatacgagcg attgcaggaa gcggcccttc gccacgccct gcggataatg
     3541 gtgggcggct ttcagcatgt agtccctggc ctccgcctgc ttgcccgcgc cgtagctgat
     3601 ggaggctgcc caggcgtacc tgttgaaata tgcccggtct ttatagtcgc gcgctttcgc
     3661 gggcaggtcg gagcgggcgt agaacttgtc cagaacgccc aaccccgccc gcgccatgag
     3721 gttgacatcg cgggaagtgt tggcggcgtg caggcggtac tcggcaagag gctcacctac
     3781 gcagtagaag cgatagcctg acgcggccat gcgtagctcc aggtcccagt cgtccgagcc
     3841 tgccagcgcg gggtcgaaca acccaacctc gtgcagcgcc cacctgggga tgaggcaggt
     3901 gcaggaggcg ataggtatat ccagcaggag gccgggcagc gcgtaccact ctcgctcagg
     3961 caagcaggct acacccgtgc ggtgcccggc ttcatctatc agatagtagc ttgaatgtac
     4021 aagggcgaca tgctcgtgcg ctctcaaagc gcgcacctgc acgtctagcc ggtggggcag
     4081 ccacacgtca tcctggtcga gcagggctat caactcgccc ctcgccgcct cgatgccggt
     4141 gttgcgcgcc gccgccaccc cgccgttctc ccgctcgatg taccttatac ggccctcgaa
     4201 ttgtgttgcg gtttgcctgg tgatagggtc gggcgagccg tcgttcacca cgataatctc
     4261 tacgttgtgg taggtctgtg atagcgcgct ctctatggcg cggacgagca tcggcccctg
     4321 gttataagca gggattatca cgctcactaa tgggttgtcg ccagcgccgc tcatgcgacg
     4381 agtatactag atacgcgtgg ttagctcaag ctatagccca ggacatcgcc cggcgagagt
     4441 gcgctacgct catagacg
//
LOCUS       DASRWZ010000368         7398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_374551, whole genome shotgun sequence.
ACCESSION   DASRWZ010000368 DASRWZ010000000
VERSION     DASRWZ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374551"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VFH60_05730"
     CDS             complement(<1..868)
                     /locus_tag="VFH60_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6313317.1"
                     /translation="MTTVLLGQSYYMRFDPKEWEGMKPYPPLGTLYAASYLRERGYDV
                     RLFDSMLSETEAEWAHALDLHKPQFAVIYEDSFNYLSKMCLSRMREAAFTMIAAGVER
                     GIPVLVSSSDATDHREKYFARGAAAIIIGEGEITLAETLDSLTGRTATPLAEIPGLAL
                     PDNPHPAKRGFIKDLDALPFPAWDLVDIERYRAVWLKHHGYYSVNMVTTRGCPYHCNW
                     CAKPIWGQRYNVRSPENVVAEMVWLRDHFRPDHLWFADDIMGLKPGWVQRFADLVAER
                     DVRLPFKSLHRVD"
     gene            complement(982..2172)
                     /locus_tag="VFH60_05735"
     CDS             complement(982..2172)
                     /locus_tag="VFH60_05735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313318.1"
                     /translation="MKLAIVIPGFQASDRDWCIPAFTNLAQELSKRNETHVFTLRYPH
                     ARGEYSIGSVRVHALGGGHLLGQRVRGISLGKLWADTLRGIAREHGRAPFDAVVGIWA
                     TESGWLAAQAARSLGVPSLVHVAGGEPVYLPSLRYGYLRKGLDGVLLNHTLAIADRIT
                     APSRQLLNTVVSEFPLAKQNAVRWPLGVDTARFARRVTQRAHNSPARFVSVGSLIPVK
                     NHTWLVRSLADLRRRRPDLDARLSIAGDGPLKPHLQRLVDKLHLMGYVTFLGEVPHDA
                     LPDLHERAHAFVLGSWHEAQCMAALEAMSAGLPWIAPAVGALADVGEDQARSDSPSGV
                     LVAERSIYAMSRAMETVADLPAEEYAKWSDGAAATVRRNYDLATQTERLERLLARLTQ
                     LTPR"
     gene            complement(2263..3726)
                     /locus_tag="VFH60_05740"
     CDS             complement(2263..3726)
                     /locus_tag="VFH60_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6313319.1"
                     /translation="MILLYNPRATEFKYRLPISVLSLAAVFDGKYEWRIVDGNIDPDA
                     GDTLLAMLERDPGIKYLLVTVMPGPQLTHAVPHTRAVKARFPHVTLVWGGYFPSSHAD
                     VVLREPSIDYAVRSQGENTILELLDTLENGGSPAGVDGLSYREGGEIRHNRARKLADP
                     NDFPVLPYEKANVPRYLKNTILGNRTISYHSSIGCPFTCSFCSVTKNYAGRWLAEKPE
                     RTIETVRYLHDRFGVNGIEFHDSNFFTAEKRVAAFAEGINGLGIGWWGEGTIDTIMRY
                     DDRTWELMRDSGCRMIFMGAENASATTLKFMSKGPLTPETTVAMAEKSRRYGIIPEFS
                     FVLGNPPDPEADIEENIEFIYKLKQINPASEIILYLYTPTPGGAMYDEAVKMGFKYPD
                     TVDEWLSPEWASFSRRRNPHTPWVKKEHLEKLNNFETVLNARYPTASDLKIRPWHRTV
                     LQTLGSWRYRLRMYDNPLELRAFFKFISYTRPEQAGL"
     gene            4137..6533
                     /locus_tag="VFH60_05745"
     CDS             4137..6533
                     /locus_tag="VFH60_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313320.1"
                     /translation="MSTLTTAATIEETRAEPRAESRARVSRWQHWHLGALLAHLGLSI
                     VVTWPLVLNFLPGSGTLVPGVMLEDRDQNLWNLWWVRRALFEGHNPFVTDLIWYPTPV
                     SLYYHTLNVFNGLLAVPLLSVFSLTTTYNLIVLFSFVMGGYGAYLLVHYLCGNRWAAL
                     VGSVVFAYSAYHVATMRSLLQLISLEWVPFFLFFLLKALFQPGWESRADFVRWLWRRA
                     LPAGFFLFLVSLVDWYYTMYALMLAGLLGVYMAVRYLWEKRRHPEIGLARGVLEPLLR
                     IGACLAIYLVLVSPILVPTIRELRQTNYMQPARDASLSNSADLLAFFQPVRGQHLWGQ
                     YFTNRREWPYGSNRYEVYFTYTALFLAGVALFATRALRPRARASNERDDAEQTRSPHA
                     SVPDAGRAPPVLSLPGKWFWAALVLLFFVLALGPVLQINGNQVLWLFSPNLPLVMPYQ
                     LLEKLPVFSISRSPDRFDMPLTLCLAVLAGYGTNVLLNTWWPRLRVSARGGLLFAGFL
                     VLVLVELTPFPYPQRPADIPRWYHQVGSEPGDFSILDLPPQDDYWHGAYRMYFQTAHG
                     KPIFGGYISREFPHPFLPSTPGYQELTYVDGAGDMFKSGPDEWLSAFQLYKTKYIVLQ
                     KVRLPDKVEPVPDIQPSREAIHYVLGADATPVYEDEQVEVYLVPSPRRTVPFMSVGEG
                     WEPREVGPNGTYRWMHGQATLRIDAPASGQAYLTFRAASLGRAKPLQVLHGDHVVFDG
                     PVGGLQPFKIGPLGIPQGVSTLTFISPEGTNSPRQLGQGDDPRQLSFVLLDAALEPVP
                     "
     gene            6597..>7398
                     /locus_tag="VFH60_05750"
     CDS             6597..>7398
                     /locus_tag="VFH60_05750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6313321.1"
                     /translation="MSSQGQVNQGPQHLTGRWYARRIRNEHGDAAAPPPAAPLPAGVF
                     DELDEANELAFGGRSSLLRVSGEGRATSRSGSPRYVGRSARHPQGMMGPTGRTGKTPS
                     TPLRLPVEPQPAPVRRFRAPTLPWSGRLVAALCVLVLATLAFRVISVPAPVIASPSGV
                     QATHAAYVKMLAGQLSPATEQGGLLLGSPPWNGVAPANPLAGLPLYHWLSALGLSLPV
                     PVEWFGRSLSALFSLLAALALFATVRRTAGSLAAVYATLFFAISPLSAV"
BASE COUNT         1239 a         2279 c         2405 g         1475 t
ORIGIN      
        1 gatcgacgcg gtggaggctc ttgaagggga ggcggacgtc ccgttcggcg acgaggtcgg
       61 cgaagcgctg cacccagccg ggcttgagtc ccatgatgtc gtcggcgaac cagaggtggt
      121 cgggccggaa gtgatcgcgc agccagacca tctcagccac gacgttctcc ggactgcgga
      181 cgttgtagcg ctggccccag atcggcttcg cgcaccagtt gcagtggtag gggcagccgc
      241 gggtcgtgac catgtttacc gagtagtagc cgtggtgctt gagccagacc gcccggtaac
      301 gctcgatgtc taccaggtcc cacgcgggga acggcagcgc gtcgaggtcc ttgatgaagc
      361 ctcgcttggc ggggtggggg ttatcgggca aggccagccc ggggatttcc gcgagtggcg
      421 tggcggtccg ccccgtgagc gagtcgaggg tctcggcaag cgttatctcg ccctcaccga
      481 tgatgatggc agcagccccg cgcgcgaagt acttttcgcg atgatcggtg gcgtcggagc
      541 ttgataccag cacggggatg ccgcgctcta cacccgccgc aatcatggtg aatgccgctt
      601 cccgcatgcg cgacaggcac atcttggaga gatagttgaa gctgtcctcg tagatgacgg
      661 cgaactgagg cttgtgcagg tcgagggcat gcgcccactc ggcttccgtc tcggacagca
      721 tggagtcgaa gaggcgcacg tcgtatccgc gctcgcgcag gtacgaggca gcatagaggg
      781 tgcccagggg cgggtacggc ttcatgcctt cccattcctt ggggtcaaag cgcatgtagt
      841 atgactggcc caggagaacg gtggtcattt acctagccct tcaagtgtgg cggctaccag
      901 tcgcaaagcg gacggcgcgc actgccattt aagtacgggg ccagttacag gcacagaacg
      961 cactatatag gggtgattgt atcagcgcgg ggtcagttga gtcaaccggg cgagcagcct
     1021 ttcgagccgc tcggtttgcg tggcgaggtc gtagttgcgg cgcacggtgg cagccgcgcc
     1081 gtcgctccac ttagcgtact cctctgctgg gaggtcggcc accgtctcca tagcgcgcga
     1141 catggcgtag atgctccgtt ccgccacgag cacccctgac ggcgagtcgg aacgcgcctg
     1201 gtcctcgccc acatccgcaa gcgcacccac cgcgggagcg atccagggca gcccggcgga
     1261 catcgcttcg agggcggcca tgcactgcgc ttcgtgccag gagcctagca cgaaggcgtg
     1321 cgctcgctcg tggaggtcgg gcagcgcgtc gtgaggcacc tccccaagga aggtgacata
     1381 tcccatgaga tgtagcttat ctacgagccg ttgcaggtgg ggcttgagag gaccgtcccc
     1441 ggcgatacta agccgtgcgt cgaggtcggg gcggcggcgt cgcaggtcgg ccaggctgcg
     1501 tacgagccag gtgtggttct tgacggggat caggctacct accgagacaa accgggcagg
     1561 tgagttatgc gccctttgcg ttaccctccg cgcgaaccgg gccgtgtcca cgcccagagg
     1621 ccacctgacg gcgttctgct tcgcaagggg gaactcgcta actacggtgt tcagcagttg
     1681 cctcgaaggt gccgttattc tatcggcaat ggccagggtg tggttgagca gtaccccgtc
     1741 cagccccttg cgcaggtacc cgtaccgtag cgagggcagg taaaccggct cgccgcccgc
     1801 gacgtgcacc agcgaaggca cccccaacga ccttgccgcc tgcgccgcaa gccagccgga
     1861 ctcggtcgcc cagatgccca ctaccgcgtc aaagggcgct ctgccatgct ccctggcgat
     1921 gcctctgagc gtatccgccc acagcttgcc caggctgatg ccgcgcactc gctgccccag
     1981 gaggtgcccg cctcctagcg cgtgcacccg caccgagcct atgctgtact cgccgcgcgc
     2041 gtgcgggtag cgcagggtaa atacatgcgt ctcgttacgc tttgaaagtt cctgggcaag
     2101 gttggtaaag gcagggatgc accagtcgcg gtcgcttgcc tggaaacctg ggatgacgat
     2161 ggcgagtttc acgatgtggc taaagtgggt acggaacgtt gagcggtgct ccgcagcagg
     2221 gggcattcga gcacagccgt ggcctgtgag gcgctgccgg cattagagtc ccgcttgttc
     2281 gggccgcgtg taagagatga acttgaagaa agccctgagt tccagcgggt tgtcgtacat
     2341 acgtaacctg tagcgccagc tgccgagcgt ttgcaggacg gtgcggtgcc aggggcgtat
     2401 cttcaggtcg gacgctgtag ggtaacgggc gttgaggacc gtctcgaaat tgttcagctt
     2461 ttccaggtgc tcctttttga cccagggggt gtgtgggttg cgccgcctgg aaaaactcgc
     2521 ccattcaggg ctcagccact cgtccaccgt gtcggggtac ttgaagccca tcttgacagc
     2581 ttcgtcgtac atcgcgccgc cgggagtagg cgtgtacagg taaaggatga tttcgctggc
     2641 ggggttgatt tgcttgagct tgtagatgaa ctcgatgttc tcctctatat cggcttcggg
     2701 gtcgggcggg ttgccaagca cgaaggagaa ttcgggtatg ataccgtagc gccgcgactt
     2761 ctcggccatc gccacggtcg tctcgggagt gagggggcct ttgctcatga acttcagggt
     2821 ggtagccgac gcgttttcgg cccccatgaa gatcatgcgg cagcccgagt cgcgcataag
     2881 ctcccaggtg cggtcgtcat agcgcatgat ggtgtctatc gtgccctcgc cccaccagcc
     2941 tatgcccagg ccgttgatgc cctccgcgaa ggccgcgacc cgcttctctg cggtgaagaa
     3001 gttgctgtcg tggaactcga tgccgttgac gccgaacctg tcgtgcaggt acctgaccgt
     3061 ttcgatcgtg cgctccggct tttcggcaag ccagcgcccc gcgtagttct tggtgaccga
     3121 gcagaaggag caggtgaacg gacagccgat gctcgaatgg taagagatgg tgcggttgcc
     3181 caggatggtg ttcttgaggt agcggggcac gttcgccttc tcataaggca gcacgggaaa
     3241 gtcgttgggg tcggccagct tacgcgcgcg gttgtgccgt atctcgccgc cctcgcggta
     3301 agagaggccg tccacacctg ccggagagcc gccgttctca agcgtgtcca gcaattcaag
     3361 gatggtgttc tcgccctgcg accggacggc gtagtctatg gagggttcgc gcagcactac
     3421 gtccgcatgc gaggaaggga agtagccacc ccaaaccaac gtgacgtgcg gaaatcgggc
     3481 cttgacagcg cgggtatgag gtaccgcgtg cgtgagttgc gggccgggca tgacggttac
     3541 gagcaggtac ttgatgcctg ggtcgcgctc aagcatcgca aggagggtat cgcccgcgtc
     3601 ggggtcgatg ttgccgtcca cgatgcgcca ctcgtacttg ccgtcgaaca cggcggcgag
     3661 tgagagcact gagatgggga gcctgtactt gaactcggtg gcccgaggat tatacagaag
     3721 gatcatggaa cctgcctggc tgaatttccg ttacacacac ggcacctgtc gcctgcaaag
     3781 gacgcgcact tattatacag catacaatcg gcggttacag cggcgttcag gatgatgagc
     3841 cgacgacgcc ctgtggctga agccaggggc tgggaagtgg caaagtctcc cgcggagact
     3901 accctcactt attgcgcact cgctgagggg actttgctcc ttgaatgccc tatggctcta
     3961 gcggaggcca tagctatgag ggcgcggacg aagaccgcac tgccgcgtta tttacgttgg
     4021 ggtttgcttt acacgccgga gtcgcgtctg ctaaaatcac agctgtgtcc aggtaccata
     4081 ctggtccccg acgatgcagg cgacgacatt tctaagcagg aagtgaaaag gcaacggtgt
     4141 ccacgcttac taccgcagca acaatagagg aaacacgtgc cgagccgagg gcggagtcgc
     4201 gtgccagggt gtcgcgatgg cagcattggc acctgggggc gttgctggcc caccttgggt
     4261 tgtctatcgt cgttacgtgg cccctggtgc tcaactttct gcccggttcc ggcacgctcg
     4321 tccccggcgt gatgctggag gaccgcgacc agaacctgtg gaacctctgg tgggtgcgcc
     4381 gcgcgctgtt cgagggccac aacccgttcg tgaccgacct catctggtac cccacgcccg
     4441 ttagcctcta ttaccacacc ctcaatgtct tcaacggcct gctcgcggtg ccgttgctct
     4501 cagttttctc cctgactacc acctacaatc tcatcgtgct cttctcgttc gtaatgggcg
     4561 ggtacggggc gtacttgctg gtgcattacc tctgcggcaa tcgttgggcc gcgctggtcg
     4621 ggagcgtggt gtttgcctac tcggcgtacc atgtcgccac gatgcgcagt ctgctccagc
     4681 tgatctcgtt ggagtgggtg cccttctttc tcttcttcct gttgaaggcc ctgtttcagc
     4741 cggggtggga gtcgcgggct gacttcgtaa ggtggttgtg gcggcgtgcg cttccggcgg
     4801 gattcttcct gttcctggtc tcgctcgtgg actggtatta caccatgtac gccctgatgc
     4861 tcgcggggct gctgggcgtc tacatggcgg tgcgctatct gtgggagaag cgcaggcacc
     4921 cggagatagg gcttgcacgg ggggtgcttg agccgctgct caggattggg gcgtgccttg
     4981 ccatctacct ggttctggtg agccccatac ttgtgcccac catcagggag ttgcggcaga
     5041 cgaactacat gcagcccgca agggacgcct cgctcagcaa ttccgcggac ctgctcgcct
     5101 tcttccagcc tgtacgcggc cagcaccttt ggggccagta cttcaccaac aggcgtgagt
     5161 ggccttacgg cagcaacagg tacgaggtct atttcaccta taccgccctc ttcctggctg
     5221 gcgtggccct gttcgccacc agggcgttgc gcccccgtgc tcgcgcctcg aacgagcgag
     5281 atgacgccga gcaaactagg tcgccccatg ccagcgtgcc tgatgccggt cgcgctccgc
     5341 ctgtgctctc cctgcctggc aagtggttct gggcggcgtt ggtgctgctg ttctttgtgc
     5401 tggcgcttgg gcccgtgctg caaataaatg ggaaccaggt actgtggctc ttcagcccca
     5461 acttgcccct ggtaatgccc taccagcttc ttgagaagtt gccggtattc agcatcagcc
     5521 gcagccccga caggttcgac atgccgctga cgctgtgcct ggcggtgctc gcgggctacg
     5581 gcaccaacgt gctgctgaac acctggtggc ctcgccttcg agtgtcggca cgcggcgggc
     5641 tgctctttgc cggtttcctc gtgctcgtgc tggttgagct tacgccgttc ccttacccgc
     5701 agcgcccggc ggacatcccg cgctggtacc accaggtggg tagcgaaccg ggcgacttct
     5761 ccatactcga cctgcccccg caggacgact actggcacgg cgcatacagg atgtacttcc
     5821 agaccgcgca cggcaagccg atattcggcg gctacatatc ccgtgagttc ccccacccgt
     5881 tcctgcccag cacgccaggc tatcaggagt tgacctatgt ggacggcgcg ggcgacatgt
     5941 tcaagtccgg ccccgacgaa tggctctcgg cgttccagct ttataagacc aagtacatag
     6001 tgctgcaaaa agtgcgcctg cccgataagg ttgagcctgt accggacatt cagccgtctc
     6061 gcgaggcgat acattacgtg ctgggggctg acgccacgcc cgtatacgag gacgaacagg
     6121 tggaggtcta cctggtcccc tcccctcgga ggaccgtgcc gttcatgagc gtaggggagg
     6181 ggtgggagcc gcgcgaagtg gggcccaacg gcacctacag gtggatgcac ggacaggcta
     6241 cccttcgcat cgacgcaccg gcctcggggc aggcatacct gacgtttcgg gccgccagcc
     6301 tgggcagggc caagccgcta caggtcctcc acggcgacca cgtcgtgttc gacggccccg
     6361 ttgggggact tcagccgttc aagatcgggc cgttgggcat cccgcaaggg gtaagcactc
     6421 tcacgttcat cagccccgaa ggcacgaata gcccacgcca actgggccag ggtgacgacc
     6481 cgcgccagct aagtttcgtg ctactggacg cggccctgga accggtgccc tagccgccag
     6541 ttatccacat gaggtgcctg tgatacaatc gctcccgtac accttcggag caagcgatgt
     6601 cgtcacaggg gcaagttaat caagggccgc aacatctcac agggcggtgg tatgcgcgtc
     6661 gaattcgcaa cgagcacggg gatgcagccg ccccgcctcc tgccgcgcca cttcccgccg
     6721 gcgtgttcga cgaacttgac gaggcaaacg agttagcctt tggcgggcgg tctagcttgc
     6781 tgcgcgtgtc gggtgagggc agggccactt cgcgctccgg gtcgccccgc tacgtgggca
     6841 ggtcggcccg gcacccgcag ggtatgatgg ggccgacggg acgcacgggc aagactccct
     6901 ccaccccgct aaggcttcca gtcgagccgc aaccggcccc cgtccgccgc tttcgcgcgc
     6961 cgacgctgcc ctggtccggc aggctcgtgg ccgctctttg cgtgctggtg ctggcgacgc
     7021 tcgcgttcag agttatttcg gtgcctgccc cggtgattgc cagcccttca ggggtgcagg
     7081 ctactcacgc cgcttatgtg aagatgctgg cgggccaact ttccccggcc acggaacagg
     7141 gcgggctact cttaggctct ccgccctgga acggggtcgc cccggccaat ccactcgcgg
     7201 ggttgccgtt gtaccactgg ctctcggctc tcggcctgag cttgcctgtg ccggtggagt
     7261 ggttcggacg gtcgctctcg gcgctctttt cgctcctcgc tgcgctggcg ctgttcgcga
     7321 cggtccgccg cacggcaggt tcgttagccg cggtgtacgc gaccctcttc ttcgcgatct
     7381 cccccctgag cgcggtac
//
LOCUS       DASRWZ010000369         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_990702, whole genome shotgun sequence.
ACCESSION   DASRWZ010000369 DASRWZ010000000
VERSION     DASRWZ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990702"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1917)
                     /locus_tag="VFH60_05755"
     CDS             complement(<1..1917)
                     /locus_tag="VFH60_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6313322.1"
                     /translation="MTRVQRSNFLPGATALERGHVGQAPRTNLPLPPTPLIGREQETA
                     AALELLRGEHVRLVTLLGPGGAGKTRLALEVARLLLPELDQGVFYVELAAITDPSLVL
                     PSIAQALGLREAAGEPVLGALHEHLRDRRVLLVLDNFEQVAQAAPQVAELLDAGPSVR
                     VLVTSRQVLGVEAERLLEVPPLRLPDRAALHGLSAEGLLEYPAVALFVQRARSIASNF
                     ELTQANAAAIAEICLRLDGIPLAIELAAARIRLLSPQALLVRLEKRLPLLTGGTQDMQ
                     PRHKTLRDTIAWSYELLDAEEQKVFRALSVFAGGLTEASAEAILDFGFWILDSETGPQ
                     IQNPKSKIQNLITSLVNKSLVRHTGESRLGMLETEREFAWQQLEASGELEQVRERHAA
                     YFLGVAERAEPELRGAEPEKWLRVLDDELDNLRAALDWSLQPGDPQLERAEVGLRIAS
                     ALQRFWQMRGHLFEGQERLARLVTATAGIGEVRSGEERLRGLAARARGLSAAGRVSHL
                     WKDLAAGREYYAESLRLHRELGNKQGMAVALMGLANIGMDAGDDLGQAGVRYDEIIAL
                     WQELDYKPGIAGALSNRGIIYARTGDYEAARDLSARSLDLYRESGDKERTAIVEDNLG
                     RIELGLGNLQGAWDL"
     gene            complement(2097..2261)
                     /locus_tag="VFH60_05760"
     CDS             complement(2097..2261)
                     /locus_tag="VFH60_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET6313323.1"
                     /translation="MRAKIEQMKKKGQGLVEYALILVLIAIVVILILTFLGNQVNTTF
                     STIGSGLTGK"
BASE COUNT          510 a          868 c          829 g          504 t
ORIGIN      
        1 caggtcccag gcgccctgca ggttacccag ccccagctcg attcgcccca ggttgtcctc
       61 tacgatggcc gtgcgctcct tgtcgccgct ctcgcggtag aggtcgaggc tgcgggcgga
      121 aaggtccctg gcggcctcgt agtcgcccgt acgggcgtag atgatgcccc tgttgctgag
      181 agcgcccgcg atacccggct tgtagtccag ctcctgccac agcgcgatga tctcgtcgta
      241 gcgaacgcct gcctgcccca ggtcgtcgcc cgcgtccatg ccgatgttcg cgaggcccat
      301 cagcgcgacc gccatgccct gcttgttgcc gagctcccgg tgcaggcgca ggctctccgc
      361 atagtactct cgccccgccg cgaggtcctt ccaaaggtgc gagaccctgc ccgccgccga
      421 gaggcccctc gccctggcag ccaacccccg caggcgctcc tcgccgctcc tcacctcgcc
      481 aatgcctgcc gtagccgtca ccagccgggc cagccgctcc tgcccctcga acaggtgccc
      541 gcgcatctgc cagaaacgct gtagggctga ggctatgcgc agcccgacct ccgcccgctc
      601 caactgcggg tcgcccggct gcaagctcca gtccagcgcg gcgcggaggt tgtcaagctc
      661 gtcgtccagc acccgcagcc acttttcagg ctcggcccct cgcagctcag gttcggcccg
      721 ctcagccact cccaggaagt aggccgcgtg cctctccctc acctgctcaa gctccccgct
      781 cgcctcaagc tgctgccacg cgaactcccg ctccgtctcc aacatcccca accgagactc
      841 gcccgtgtgc ctcaccaggc tcttgttcac cagagaggtg attagatttt ggattttaga
      901 ttttggattt tggatttgcg gacccgtctc tgaatccaaa atccaaaatc caaaatctaa
      961 aatggcttcc gcagaagcct cagtaaggcc tcctgcgaac acggagaggg cgcggaagac
     1021 cttttgctcc tcggcgtcca gcagctcgta gctccaggct atggtgtcgc gcagggtctt
     1081 atgccggggc tgcatgtcct gggtgccgcc ggtaaggagg ggcagccgct tctctaggcg
     1141 aacgaggagg gcctggggcg agaggagccg gatgcgggcc gccgcaagct cgatggccag
     1201 ggggatgccg tcgaggcgca ggcagatttc ggcaattgcg gcggcattgg cctgggtaag
     1261 ctcgaaattg gaggcgatgg accgcgcgcg ttggacgaac agggccactg ccggatactc
     1321 caacaggccc tcggcgctga ggccgtggag cgcggcacgg tcgggcagcc ggagtggggg
     1381 tacttccagc aggcgctcag cctccacgcc cagcacctgc ctgctcgtca ccagcaccct
     1441 gacagaaggc ccggcgtcca gcaattccgc cacttgaggg gccgcctgcg ctacctgctc
     1501 gaagttgtcc agcaccagga gcacgcgccg atcgcgcaaa tgctcgtgga gcgcgcccaa
     1561 caccggttct cccgcggcct cacgcaggcc gagcgcctgg gcgatactag ggagcacgag
     1621 cgaggggtcc gtaatggctg ccagttccac gtagaagacc ccctggtcga gctccggcag
     1681 cagcaggcgg gctacctcca gcgccaggcg cgtcttaccg gcgccacccg gccccagcaa
     1741 ggtcacgagg cggacgtgct ctccacgcag caactccaac gcggcggccg tttcctgctc
     1801 gcgcccaatg agaggggtag gtggcagcgg aaggttggtg cggggtgcct gccccacgtg
     1861 gccgcgctct aaggcagtcg cgccgggtag gaagttggaa cgttgtaccc tggtcatggc
     1921 ggcgtggaaa tgtcggatac ctggcgaggg gagtaccggc accggcgcgt acggcatgcc
     1981 agggccggtg ggaagtaagt tgcctgcttt gattatatcg gaacccgaag attaagccag
     2041 ttaacgcaag aaagacccct aagggtctct cctggttatg gactagtggg tgtgagttac
     2101 ttgccggtga ggccggagcc tatggtggag aaggtggtgt tgacctggtt gccgaggaaa
     2161 gtcaggatca agataaccac gatggcgatc agcaccagga tcagggcgta ctctaccagg
     2221 ccctggccct tcttcttcat ctgctcaatc ttagcgcgca ttgtttttgt ctccttgtgg
     2281 aactgaaaaa tgatggccgt ataagtcctc gtcggtgcta ataagtgccc cgaccttcac
     2341 tcacatcgta cagttatttc gcgcccggta gaatagcccg ctggtactaa tcagagcaaa
     2401 attaagtggc tacatggtac tagcccaaac tagtactatg ctaacgtaca gtcctggtcg
     2461 ctcgtcagtt ccgccaggaa aggtaagcgt tcagaaaaaa aggtagtgca aaaggtaaga
     2521 gagcatcgca ccctctgagc tggtaggtag ccaacatgtt ccaatgtgtt ggcaccaaaa
     2581 ctccttcctg tcagcctgta ttcccccctt gtcatcctga gcgtagcgaa ggatcaggtg
     2641 ggtaggcgag actctacaac gtaggggatg atgtaggcat taggaacaga ggtgcagcaa
     2701 tgtgaagagt c
//
LOCUS       DASRWZ010000370         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_990762, whole genome shotgun sequence.
ACCESSION   DASRWZ010000370 DASRWZ010000000
VERSION     DASRWZ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990762"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..2005
                     /gene="trpE"
                     /locus_tag="VFH60_05765"
     CDS             350..2005
                     /gene="trpE"
                     /locus_tag="VFH60_05765"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET6313324.1"
                     /translation="MILRTWLRAHMSLALTDRWRKGTTVSQAYSPTREAFRAFAQEQQ
                     AGGTGAEPYTLVSRQIPADLETPVSALLKIRRGEHCFLLESVERGTQVGRYSFLGTGP
                     QSIFTVKDGQSRIDTPEGRGLSAESTDPLGELDALLASRRLVVPEGLPVPRFAGGAVG
                     YAGYETACTLEPVRPAANDLLGLPEMSFAFYDTFLVFDHAQRSARVYSLAPLSGDPDV
                     EYAAAVERIEQLVGRLRRPLPESNGHRRHGEIAARSPVSSNFTQAEYEGIVEAAKEHI
                     AAGDIIQVVVSQRFDRPTGADAFSIYRALRMVNPSPYMFFLQFGDTYLVGASPEMLVQ
                     VQEGKLAMHPIAGTRPRGVDMREDARLAKELASDEKERAEHVMLVDLGRNDIGRIARI
                     GSVKVPRFMEVEQYSHVMHLVSEVTGDLRPELGAVDALRACLPAGTLSGAPKVRAMQI
                     ISELERDRRGPYGGAAGYISYNGNLDTAIIIRTIVVRDGMAHLQAGAGIVADSVPEME
                     YRETLAKAKVLIAAIDLAEEMESEHTALEYATVDEPAPEGASK"
     gene            2002..2586
                     /locus_tag="VFH60_05770"
     CDS             2002..2586
                     /locus_tag="VFH60_05770"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET6313325.1"
                     /translation="MILLIDNYDSFTYNLYQYLGELGSRVEVVRNDAATVAELELMRP
                     DGVVISPGPGAPDSAGVSLQAIEAFAGRVPVLGVCLGHQCIGQLYGGKIVRAPELRHG
                     KTSEIYHTGKGVFTGLPSPFVATRYHSLIVDAATCPRELEVTAWTGDGLIMGLRHKRL
                     PVEGVQFHPESMLTQSGKALLSNFLAQVNAFAAV"
     gene            2713..3270
                     /locus_tag="VFH60_05775"
     CDS             2713..3270
                     /locus_tag="VFH60_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HET6313326.1"
                     /translation="MTEEQILASSVVPPTPLVGRIRLEEYNPDWPRLFEREAARIRAA
                     LGDQALQVEHAGSTSVPGLAAKPLIDIVLVVLNSADEAAYVPALETAGYTLTIREPDG
                     YEHRLFKGPDTNVNVHTFSAGCPEIERMLLFRDWLRTHPDDRERYERTKRELAMQDWK
                     YVQNYADAKTALVEDILARANGSRR"
BASE COUNT          678 a         1057 c         1125 g          579 t
ORIGIN      
        1 gtctccctct tgacagcacc ctcatttgct tgtagactag gcaataaccg aataggcaga
       61 acgactacga ccagcacgag tagcaggcga ggcgagtccc cagagagttg gcggctgtga
      121 aagtccaacg gcggcgcgac ctgtgaatgg agctgtgagt tgcacaccga aagccgcgcg
      181 agcgtgcgag tagactgtgc cggagactcc accgttacct gggagcggga tatcggcaga
      241 catggcgcgt acgcgtcctg actaggtatc cgcggagcgc cgcacgacgg cggaaaaagg
      301 gtggcaccac gaagctcagt ccttcgtccc ttgcggacgt tgggcttttt tgattcttcg
      361 cacttggctg cgggcgcaca tgtcgctcgc actgaccgac cgctggagaa aggggacaac
      421 cgtgtcgcaa gcatattcgc caacgaggga ggcgttccgc gccttcgcac aggagcaaca
      481 ggccggcggc accggcgctg agccgtatac gctggtcagc aggcagatac ccgccgacct
      541 cgagacgccc gtgtcggccc tgctcaagat acggcgtggg gagcactgct tcctgctcga
      601 gagcgtggag cgcggcaccc aggtggggcg ctattcgttc ctgggcacgg ggccgcagtc
      661 catctttacg gtgaaggacg ggcagtcgcg catagataca ccggaggggc ggggcctctc
      721 agccgagagc accgacccgc ttggcgagtt ggacgcactg ctggcttcgc ggcggctggt
      781 ggtgccggag gggctgccgg tgcccaggtt cgcgggcggg gcggtcggct acgcggggta
      841 tgagaccgcc tgcacgctgg aacctgtgcg ccccgctgcc aacgacttgc tcggcctgcc
      901 ggaaatgtcg tttgccttct acgatacgtt cctggtgttc gaccacgccc agcgtagcgc
      961 gagagtgtac agcctcgccc cgctcagcgg cgaccccgac gtggagtacg cggcggcggt
     1021 cgagaggatc gagcagctag tggggaggct gcgcaggccc ctgcccgaaa gcaacggaca
     1081 caggcggcat ggtgagatag cagccaggtc gcccgtcagc agcaatttta cgcaggccga
     1141 gtacgagggc atcgtggagg cagccaagga gcatatcgcg gcgggggata tcatccaggt
     1201 ggttgtatcg caacgctttg acaggcccac gggcgcggac gccttcagca tctaccgggc
     1261 cttgcgcatg gtgaacccct cgccctacat gttcttcctg caatttggcg atacgtacct
     1321 ggtgggggcc tcgcctgaga tgctggtgca ggtgcaggag ggcaagctag ccatgcaccc
     1381 catagcgggc acccgcccca ggggcgtcga tatgagggag gacgcccggc ttgccaaaga
     1441 actggcctcg gacgagaaag agcgtgccga gcacgtcatg ctggtggacc tggggcgcaa
     1501 cgacataggc cgcatcgccc gcatcggctc ggtgaaggtg ccccgcttca tggaggtgga
     1561 gcaatattcc cacgtcatgc acctggtgtc ggaagtcacg ggcgacctgc ggccggagct
     1621 tggggcggtg gacgccttga gggcttgctt gccggccggt acgctgtcgg gcgcacccaa
     1681 ggtgagggcg atgcagatca tctcggagct tgagcgggac aggcgagggc cttacggggg
     1741 cgcggcgggc tacatcagct acaacggcaa cctcgacacg gccatcatca tccgcaccat
     1801 tgtggtgagg gacggcatgg cccacttgca ggcgggcgcg ggcatcgtgg ccgatagcgt
     1861 gcctgagatg gagtaccgcg agacgctggc gaaggcaaag gtgctgatcg cggcgataga
     1921 cctcgcggag gagatggaga gcgagcacac ggcactcgaa tacgcgaccg tagacgaacc
     1981 ggcaccggag ggagcaagca aatgatactt ctcatcgata actacgactc gttcacctac
     2041 aacctatacc agtacctggg cgagcttggc agccgcgtgg aggtggtgcg caacgacgcc
     2101 gcaacagtag ctgagctaga gttgatgcgc cccgacggcg tggtaatctc ccccggcccc
     2161 ggcgcgcccg acagcgcggg agtgtcgctt caggcgatag aggcgttcgc gggcagggtg
     2221 ccggtgctgg gcgtgtgcct gggccaccag tgcatcgggc agctatacgg cggcaaaata
     2281 gtgcgcgccc cggagctgcg gcacggcaag acctcggaga tataccacac cggcaagggc
     2341 gtgttcaccg gcctgccctc tccgttcgta gccacccgct accactcgct catcgtggac
     2401 gccgccacgt gcccccgcga gctagaggtc accgcctgga ccggcgacgg cctcatcatg
     2461 ggcctgcggc acaagcgcct ccccgtagaa ggcgtgcagt tccacccgga gtcgatgctc
     2521 acccagtccg gcaaagccct gctctccaac ttcctggcac aggtgaacgc cttcgctgcc
     2581 gtatgagcat tcgtgcacca ggatgttgtt agagaagggg actaccaagt gagcaccggc
     2641 aaaccggaca atgacccgcc gggggcgcag agtaccccgc gtaatgaagt tgcgtccgac
     2701 aatcgagcgc cgatgaccga ggaacaaatc ttagcgtcct cggtcgttcc cccgaccccg
     2761 ctcgttggcc gcatccggct tgaagaatac aatcctgact ggccgcgcct cttcgaacgc
     2821 gaggctgccc gcatccgtgc ggcactggga gaccaggcgt tacaggtgga acatgccggg
     2881 tccacctctg ttcccggcct ggcggccaag cccctcatcg atatcgtcct ggtcgtcctg
     2941 aactcggctg acgaggcggc ctatgtacca gcattggaga cagcagggta caccctcact
     3001 attcgcgaac ctgatgggta cgagcaccgg ctgttcaagg ggcctgatac caacgtcaat
     3061 gtccatacct tctccgccgg ttgtcctgag atcgagcgga tgctgctctt tcgcgattgg
     3121 ctgcgcaccc acccggacga ccgcgaacgg tacgaacgaa caaaacgtga gctagccatg
     3181 caggactgga agtacgtaca aaattacgcc gacgccaaga cggccctcgt ggaggatatt
     3241 ctcgctcgcg caaacgggtc caggcgatag tttacagtac cgcctgggtt ttctcagcag
     3301 cttctagtac aatgccaaca aattcgcatt ccgaaccaac tgcctggcgc aagtaaacgc
     3361 atttgtggca ggcgtataaa acggaccagg aactgggact tcggacttag cggcgcacct
     3421 gaacgtgtct ttcctacta
//
LOCUS       DASRWZ010000371         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1059409, whole genome shotgun sequence.
ACCESSION   DASRWZ010000371 DASRWZ010000000
VERSION     DASRWZ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059409"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..599)
                     /locus_tag="VFH60_05780"
     CDS             complement(75..599)
                     /locus_tag="VFH60_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6313327.1"
                     /translation="MLTQPAIIVITGIQAAGKSTVARLLAARFERGVHVEADALQRMI
                     VSGGEWVGEPGPPSAEAERQLRLRLRNMCLLGKSFYDHGFSVVLDDIIIGDRWQHLQE
                     ELEGYPFTLVVLAPHTEVVQDRDARRGKPPLGARWAVYLDDELRKTMMGIGTWIDSSG
                     QTPDETVDAVLARL"
     gene            complement(656..1381)
                     /locus_tag="VFH60_05785"
     CDS             complement(656..1381)
                     /locus_tag="VFH60_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HET6313328.1"
                     /translation="MPLKPLIILPTYNEIENLQPLVEEILQRGPYHVLVVDDNSPDGT
                     GRLADSLAKAHEGRVFVLHREKKEGLGRAYVAGFRWGLARDYDVLFEMDCDFSHDPAH
                     LAQFMREIENGADLVLGSRNIRGGGTRNWSLLRKIISRGGSLYAQAILLVPYRDLTSG
                     FKAFRRKVLESLDLDSIGSNGYSFQIEVTYRAHQLRFKIKETPIIFVDRRVGHSKMSG
                     NIVSEAMGVVWKIRFSKPPRRRR"
     gene            complement(1434..>2113)
                     /locus_tag="VFH60_05790"
     CDS             complement(1434..>2113)
                     /locus_tag="VFH60_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313329.1"
                     /translation="APFWEGWQSIGALGRQSLFTASIPKVILDMLVLNYGIDVPTAES
                     LVRNGALSLVGLVTLWVAWRVFRVRNATTPAERTRLLERTLAGFYEVIFVYLAFGTLW
                     FQPWYLMWLVALTAPVARYVNANRTLLFCMGGIANYFVWDFIWLWNRTSSRHIQFTAA
                     LVVYTWPLFYSLYAWLSPVWRAREAEAEEDGDLEEIDEIEAMPEATEAETDIATPPGR
                     VQQPTHS"
BASE COUNT          379 a          664 c          664 g          406 t
ORIGIN      
        1 gggagctgga ggacgagatt tctcgctgcg ctcagaatga cagaggagcg gggttatgta
       61 cccataagca tgcgtcaaag acgagccagg accgcatcca ccgtctcgtc cggcgtctgg
      121 ccggagctat caatccaggt accgatgccc atcatggtct tgcgtagctc gtcgtccagg
      181 tataccgccc aacgcgcacc caatggcggc ttgcctcggc gggcgtctcg gtcctgcaca
      241 acctccgtgt ggggtgccag cactaccagc gtgaaggggt agccctctag ctcctcctgt
      301 agatgctgcc agcggtcgcc tatgatgatg tcgtccagca ccaccgagaa gccgtggtcg
      361 tagaacgact tgccaagcag gcacatgttc cgcaggcgca gtctaagctg ccgttccgcc
      421 tccgccgagg gtggccccgg ttcgcccacc cactcgccgc cggagacgat catccgctga
      481 agcgcatcgg cctcgacgtg caccccgcgc tcaaaccggg ccgcaagcaa ccgtgcgacg
      541 gtgctctttc ccgcagcctg tatgccggtt atgacaatga ttgccggttg ggtaagcaat
      601 gcgttgtttg ggactatgga cgatagacga tggacgatat gagacctccc atcgtctatc
      661 gtctgcgcct tggcggcttg ctgaagcgaa ttttccagac gacccccata gcctccgaca
      721 cgatgttgcc gctcatcttg ctgtggccga cacggcggtc cacgaagatg atgggggtct
      781 ctttgatctt gaagcggagt tggtgcgccc ggtaggtcac ttcgatctgg aacgagtagc
      841 cgttggagcc tatgctgtcg aggtcgagcg actcgagtac cttgcgcctg aacgccttga
      901 agccgctggt gaggtcgcgg tagggcacca ggaggatggc ctgggcgtag agcgagccgc
      961 ccctgctgat gattttacgc aacagcgacc agttgcgcgt gcccccaccc ctgatgttgc
     1021 gcgagccgag caccaggtcg gccccgttct ctatctcgcg catgaactgg gccaggtgcg
     1081 ccgggtcgtg ggagaagtcg cagtccatct cgaacagcac gtcgtagtcg cgagccaggc
     1141 cccacctgaa gccggccacg taggcgcggc ccaggccctc cttcttctcg cgatgcagaa
     1201 cgaacacgcg gccctcgtgc gctttcgcca ggctgtcggc cagtcgcccc gtaccgtcgg
     1261 gcgagttgtc gtccaccacg agcacgtggt acggcccacg ttgcagtatc tcctccacga
     1321 ggggctgcaa gttctcgatt tcgttgtacg tgggtaggat aatcagcggt ttgagtggca
     1381 aatgtttgct cctgcggacg gctgggcgta gcgcgtggcc ggtgcgcgtg tgtttagcta
     1441 tgtgtaggct gctggacgcg gcctggcggt gtggcaatgt cggtttcggc ctctgtggct
     1501 tccggcattg cctctatctc gtctatctct tctaaatctc catcctcctc ggcttcggcc
     1561 tcacgcgcgc gccagacagg gctgagccag gcgtataacg aatagaacaa cggccaggtg
     1621 tataccacta gcgccgccgt gaactggatg tgccgcgacg aggtccggtt ccagagccat
     1681 atgaagtccc acacgaagta gttggcgatg ccccccatgc agaagagcag ggtgcggttg
     1741 gcgttcacgt agcgggccac gggcgcggtc agggccacca gccacatcag gtaccagggc
     1801 tggaaccaga gcgtgccgaa agccaggtag acgaatatta cctcgtagaa gccagcgagc
     1861 gtgcgctcca ggagccgggt gcgctcggcg ggcgtggtgg cgttcctcac ccggaagacg
     1921 cgccaggcca cccataatgt gaccaggccc accagcgaca gcgccccgtt tcggaccagc
     1981 gactccgcgg tcggcacgtc gatgccgtaa ttgagcacca gcatgtcgag tatgaccttg
     2041 ggtatcgagg cggtgaacag gctctgccgc cccagcgccc cgatgctctg ccagccctcc
     2101 cagaacggcg cgt
//
LOCUS       DASRWZ010000372         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_785533, whole genome shotgun sequence.
ACCESSION   DASRWZ010000372 DASRWZ010000000
VERSION     DASRWZ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785533"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..506
                     /locus_tag="VFH60_05795"
     CDS             204..506
                     /locus_tag="VFH60_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HET6313330.1"
                     /translation="MIKLVALFKRPQDTGEFDAHYADVHAPLMRKVPGLERMEVTRNL
                     QAFRGEPEYYLVAEMYFRDRESFDAAMSSEENRAAGKDLMSFAREYVTMFYGEVEG"
     gene            509..1462
                     /locus_tag="VFH60_05800"
     CDS             509..1462
                     /locus_tag="VFH60_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6313331.1"
                     /translation="MDNSRYPDYPRPLSPGELGFSRILYTKAEGVATVTINREPVMNA
                     TDFHTLKEMSRAFEDASYDDAVGVVVLTGAGKRAFCTGADLDEQEQFLRKRADYWKWM
                     GAFIEAHDRLRNIGKPTVARLNGMVVGGGNEFNIACDLAIAAEHVTIRQVGAARGSVP
                     AGGATQWLPLIIGDRRAREMLWLCEPISATTALDWGLVNEVVPFEDLDAAVGRMTRKL
                     LNKMPEIIRYAKQQTNFWRDFSWSLTVGHARDWLSVHADAEETAEGLASFHAKREVDY
                     PMLRRHMAEGSRACHNCGNPVPAQYAYCGACGTKQEGAENG"
     gene            1455..2237
                     /locus_tag="VFH60_05805"
     CDS             1455..2237
                     /locus_tag="VFH60_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6313332.1"
                     /translation="MADSSYSLIKLSQDGPVTIIQLNRPDVLNALNLQLMDEIIAALD
                     TLEADDTVRCVVLTGSERAGAAGADIKEMAGATTVDMMMRDQFAKWDRIRRFKKPIIA
                     AVSGYALGGGNELAMLCDMIVASETAQFGQPEINIGVMPGAGGTQRLTRAVGKAIAMD
                     MVLTGRMLSAREALQFGLVNRIVPVEAYLKEAVKLAKSIAEKSPIAVQMAKDAVLKAF
                     DTPIQEGLLHERKNFYMLFSTEDQKEGMAAFIEKRKPEFKGK"
     gene            2240..3022
                     /locus_tag="VFH60_05810"
     CDS             2240..3022
                     /locus_tag="VFH60_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6313333.1"
                     /translation="MDYETILYNLEEGVLAITLNRPDVLNAFNRKMTDELQDAFKKAE
                     RDPAVRCIVLTGAGRAFSSGEDLKSRSAEGDSDFGSTLRQRYNPLVSKMRNIEKPVLG
                     SINGVAAGAGCSIALACDLRIASEKARFMEVFVRVGLVPDSGSSFLLPRLVGLGRALE
                     MAFLGDEMGAEEALRTGLVNRVVPAGELESATRELALRLAKSPTKAIGLAKRAINRAL
                     TMDLDQALDYEVYGQETAGASDDHKEGIAAFLEKRTPNFTGR"
     gene            3029..>3706
                     /locus_tag="VFH60_05815"
     CDS             3029..>3706
                     /locus_tag="VFH60_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET6313334.1"
                     /translation="MPGKIAVLGAGTMGAGIAQLAAQSGFDVLLYDIKEDFVQRGTGN
                     IRSALQGRVDKGKMSREEFDAITARISTTTVRDDAAKADFIIEAAPEDIALKREIFSS
                     LSKLSAPDTVLASNTSSLSITSIASAADRPESVVGLHFFNPAPVMPLVEVIAGARTSA
                     GVREAAAQLARDMGKTPVLAADSPGFIVNRVARPFYGEALKMLGEGFAGVAQIDASMR
                     AAGFKMGP"
BASE COUNT          721 a         1208 c         1180 g          597 t
ORIGIN      
        1 gagacatact accaaacgtt tggcatcgcg gcaaaggcag catcttagat ccttcgctac
       61 gctcaggatg acagggggag catggtcgcc acacttcaca cgaacgcccc ttgggccaat
      121 ccgcctctgc gggtgcgctc ccttgacaag cgacaagccc ctgtgataac cattcaaggc
      181 aattctacga ggaggtagaa gaaatgataa agttggtggc cctcttcaag cgcccccagg
      241 atacaggcga gttcgacgcg cactacgccg acgtgcacgc ccccctcatg cgcaaagtcc
      301 ccggcctcga acgtatggaa gtcacccgca acctccaggc gttccgtggc gagcctgagt
      361 actacctggt ggctgagatg tacttccgcg accgcgagtc cttcgacgcc gctatgtcgt
      421 ccgaggagaa ccgcgcggcg ggcaaggacc tgatgagctt cgcccgcgag tacgtgacca
      481 tgttctacgg cgaagtggag ggctaacggt ggacaactcc aggtacccag actacccccg
      541 ccccctctcg cccggcgagc taggcttctc ccgcatcctc tacaccaagg cggaaggcgt
      601 ggctacggtc accatcaacc gcgagccggt gatgaacgcc accgacttcc acaccctcaa
      661 ggagatgagc cgcgcctttg aagacgcttc ttacgacgac gcggtgggcg tggtggtgct
      721 caccggggcg ggcaagcggg ccttctgcac cggggccgac ctcgacgagc aggagcagtt
      781 cctccgcaag cgggccgact actggaagtg gatgggcgct tttatagagg cccacgaccg
      841 cctccgcaac atcggcaagc ccaccgtggc ccggctcaac ggcatggtcg tgggaggcgg
      901 caacgagttc aacatcgcct gcgacctggc aatcgccgcc gagcacgtga ccatcaggca
      961 ggtcggtgcc gcccgtggct cggtgcccgc cggtggggcc acccagtggc tgcccctcat
     1021 cataggcgac cgccgcgccc gcgagatgct ctggctgtgc gagccgattt ccgctactac
     1081 cgccctcgac tggggcctgg tcaacgaggt agtgcccttc gaggacctcg acgccgccgt
     1141 tggcaggatg acgcgcaagc tgctcaacaa gatgcccgaa atcatccgtt acgccaagca
     1201 gcagaccaac ttctggcgcg atttctcgtg gagccttacc gtggggcacg cccgcgattg
     1261 gctatccgtg cacgccgacg ccgaggagac cgccgagggg ttggcctcct tccacgccaa
     1321 gcgcgaggta gactacccga tgctccggcg gcacatggcc gaaggctccc gcgcctgcca
     1381 caactgcggc aaccccgtac cggcccagta cgcctactgc ggcgcgtgcg gcacgaagca
     1441 ggagggcgcg gagaatggct gacagcagct acagcctcat caagctgtct caggacggcc
     1501 ccgttaccat catccagctc aaccgccctg atgtgctcaa cgccctcaac ctgcaactga
     1561 tggacgagat tatcgcagcc ctcgacacgc ttgaagcgga cgatacggtg cggtgcgtgg
     1621 tgctcacggg cagcgagcga gccggcgcgg cgggggccga catcaaggag atggccgggg
     1681 ctaccacggt cgatatgatg atgcgcgacc agttcgccaa gtgggaccgc atacgacgat
     1741 tcaagaagcc catcatcgcg gccgtcagcg gctacgccct gggtgggggc aacgagcttg
     1801 ccatgctctg cgacatgata gtggcctccg agaccgccca gttcggccag cctgagatca
     1861 acataggcgt catgccgggc gcgggaggca cccagcgtct cacccgcgcg gtcggcaagg
     1921 ctatcgctat ggacatggtg ctcacggggc gcatgctgag cgcgcgggaa gccttgcagt
     1981 tcggcctggt gaaccgaatc gtgccggtgg aggcgtacct caaagaggcg gtgaagctgg
     2041 cgaagagcat cgcggagaaa agccctattg ccgtgcagat ggccaaggac gcggtgctca
     2101 aggcgttcga cacgccgata caggagggcc tgttgcacga gcgcaagaac ttctacatgc
     2161 tcttcagcac cgaggaccag aaagagggca tggcggcctt catcgagaag cgcaagcccg
     2221 agttcaaggg gaaataagca tggactacga gacgattctc tacaacctcg aggagggcgt
     2281 gctcgcaatc acgctcaacc gccccgacgt gctcaacgcc ttcaaccgca agatgaccga
     2341 cgagctacag gacgccttca agaaggccga gcgcgacccc gccgtgcgct gcatcgtgct
     2401 tacgggcgcg ggccgggcct tctcgtccgg cgaggacctc aagtcccgct cggcggaagg
     2461 cgactccgac ttcggctcca ccctgcgcca gcgctacaac cccctcgtgt cgaagatgcg
     2521 caacatcgaa aagccggtgc taggctcgat caacggcgta gctgccgggg ccgggtgctc
     2581 tatcgccctg gcctgcgacc tgcgtattgc atcggagaag gcgcggttca tggaggtgtt
     2641 cgtgcgggtg gggcttgtac ccgactccgg ctcgtcgttc ctcctgcccc ggcttgtggg
     2701 cctcggtaga gccttggaga tggccttcct gggggatgag atgggagcgg aggaggcgtt
     2761 gcgcacgggg cttgtcaatc gcgtggtgcc cgccggggag ttggagagcg ccacccgcga
     2821 gttggccctg cgcctcgcaa agtcccccac gaaagcgatt ggactggcaa agcgcgccat
     2881 caaccgtgcc ctcacaatgg acctcgacca ggcgctcgac tatgaggtgt acggccagga
     2941 gactgccggg gcctccgacg accacaaaga gggtattgcc gcttttcttg agaagcgcac
     3001 ccccaacttc acaggcaggt aagcagatat gccaggcaaa atcgcggtgc tcggcgcggg
     3061 cacgatgggc gcgggtatag ctcaactagc ggcacagagc ggcttcgacg tgctgctcta
     3121 cgacatcaag gaagatttcg tgcagcgcgg caccggcaac atccggtcgg cattgcaggg
     3181 ccgcgtcgat aagggcaaga tgtcgcggga ggagttcgac gcaattactg cccgcatcag
     3241 cacgaccaca gtgcgcgacg atgccgccaa agccgacttc atcatagagg ccgcgcccga
     3301 agacattgcg ctcaagcggg aaatcttctc ctccctcagc aagctgtccg ctcccgatac
     3361 ggtcctggcc agcaacactt cctccctgtc gattacctcc atcgcctcgg cggcggaccg
     3421 cccggagtcg gtggtaggac ttcacttctt caacccggcc cccgtcatgc cgcttgtcga
     3481 ggtgatagcg ggcgcgcgga cctctgccgg cgtgcgcgag gcggcggccc agcttgcgcg
     3541 cgacatgggc aagacgccgg tgctcgccgc cgacagcccc ggcttcattg tcaaccgggt
     3601 ggcgcggcct ttctacgggg aagccctcaa gatgctgggt gagggcttcg caggcgtcgc
     3661 ccaaatcgac gcgagcatgc gggccgccgg gttcaagatg gggccg
//
LOCUS       DASRWZ010000373         4124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_100376, whole genome shotgun sequence.
ACCESSION   DASRWZ010000373 DASRWZ010000000
VERSION     DASRWZ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100376"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1873)
                     /locus_tag="VFH60_05820"
     CDS             complement(125..1873)
                     /locus_tag="VFH60_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HET6313335.1"
                     /translation="MRLRTHNIYIALAILAASLLLASLNGSRPAEAAQGSAPVRWAFY
                     VTYNPNSLVSLQANVHNLNYVSPWFYTVNMTGTVSGKDQAAASALIRNAGAKTLPMLQ
                     NTAQYNAFSPVLTDTLKQQSLVNQIDNIVTANNYDGITIDFEGLNPSDKPHLTAFMGK
                     LYERLHPKGKLVAMAVAPKSRDVTTGWAAAYDYAALAQYTDYLLVMAYDYHFAGGDPG
                     PIAPLYRLHDAAKYALARVPASKLIWGVGVYGYDWPTNPEGKSTAHAEYLTYEQAQAI
                     TQRPGSSSGYDQEAQAPFVRYTRNEQTREAWYENRRSFEAKLDLISRYNFAGFGIWRL
                     GQEDPAIWQSINGDRLPAQQPQPTNTPARTVPPTSTTAPTFTPVPPTATPTPTPPPAC
                     LPVQPFKSSADKLYFQPTGHSLRGIFLQYWQKNGGLPIFGYPLTEEFTEVSPTDGKPY
                     KVQYFERNRFEHHPEYAGTPNEVLLGLLGVQALGGRVFPEAAEIETGPDTIYFPQVKH
                     SLSGPFLKYWQGNGGLTRLGYPVTEPVLEQSSIDGRTYSVQYTERARLEFHPEYAGTQ
                     YEVLLGLLGLDISPCR"
     gene            2328..2912
                     /locus_tag="VFH60_05825"
     CDS             2328..2912
                     /locus_tag="VFH60_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6313336.1"
                     /translation="MAHTRRIVEEAEQAPEPGHGGLLEDAAAELLSVLPRMIRVIKHS
                     ARYGEQSEQAGPLRELGESQVMVLFTLRHQGTQLTSELARRYNVTNPTMTRIVDELVK
                     KGLVVRQPDTKDRRCIFLELTEEGRELAAVAQEHGRKALVEYLQPLNNEELADVSRAF
                     GHLRRLLPEALRDPASCSVRRMEQAHVEGGRGLN"
     gene            2965..>4124
                     /locus_tag="VFH60_05830"
     CDS             2965..>4124
                     /locus_tag="VFH60_05830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6313337.1"
                     /translation="MNSEATQEAEGQGKPVGAATGTQYMGDETPRLPRRQLAAIMISV
                     LLGLLLSALDQTIVGPAMPRIIGDLNGFEAYSWVFTVYLLTSTITVPIFGKLSDMYGR
                     KWFYVGGILVFLLGSVLSGLSADMAQLIAFRGLQGIGAGIMMATAFAIIADLIPPSER
                     GKWQGAFGAVFGISSVIGPTIGGFLTDNLNWRWVFYVNMPVGAIAIAALVLLFPNDRK
                     VQHARRSIDWWGAGTLVAGLTPLLLALSLVGTATRVELPGVGLITSWGWDSPTILALL
                     AMSVAFLVAFVYVQGRAKEPIIPLDLFKHNIFTVSVITVFLTGVGMFGAVLYIPLFIQ
                     AVQGDSATNSGNSVTPMTLAIVLSSIVTGQIISRTGKYRIIGIVGMVMVTGGA"
BASE COUNT          696 a          987 c         1581 g          860 t
ORIGIN      
        1 actacatgat gtgccttagt cccccagggg actttgcctc ttgtaagccc gtggctttag
       61 aggcagggcg tcttgcatgc ccagctacgg cttcagtgac gaggctgatt aagccctgct
      121 ttcatcagcg gcagggggag atgtccagtc cgaggaggcc gaggaggact tcgtactggg
      181 tgcctgcgta ttcggggtgg aattcgaggc gggcgcgttc ggtgtactgg acgctgtagg
      241 tgcgcccgtc gatgctgctt tgttcgagga caggctcggt gacggggtag ccgaggcggg
      301 tgaggccgcc gttgccctgc cagtacttga ggaaggggcc gctcaggctg tgcttgacct
      361 gggggaagta tatggtgtcg gggccggtct ctatctcggc ggcttcgggg aagacgcggc
      421 cgccgagcgc ctgcacgccg aggaggccaa gcagcacttc gttgggggtg cccgcgtatt
      481 cggggtggtg ctcgaagcgg ttgcgctcga agtactgcac tttgtagggc ttgccgtctg
      541 tgggcgacac ctcggtgaac tcttcggtga gcggatagcc gaagataggg aggccgccgt
      601 ttttctgcca gtattggagg aatatgccgc gcaggctgtg gccggttggc tggaagtaga
      661 gcttgtccgc ggacgacttg aagggctgca cgggcaagca ggcaggtgga ggggtgggag
      721 taggcgtggc ggtgggaggt acgggcgtga aggtaggggc ggtggtagag gtgggtggca
      781 ctgtgcgggc tggggtgttc gtgggttggg gctgctgggc gggtaggcgg tcaccgttga
      841 tggactgcca gatagcgggg tcttcctggc cgagccgcca gatgccgaag cctgcgaagt
      901 tgtagcgact aatgaggtcg agcttggcct cgaagctgcg gcggttttcg taccaggcct
      961 cgcgggtctg ctcgttgcgg gtgtagcgga cgaagggggc ctgggcttcc tggtcgtagc
     1021 cgctggacga gccgggccgc tgggtgatgg cctgggcctg ttcgtaggtg aggtactcgg
     1081 catgggcggt tgacttgccc tcggggttgg tgggccagtc gtagccgtag actccgacgc
     1141 cccagattag cttgcttgcc ggtacgcgag ccagggcgta cttggccgca tcgtgcagcc
     1201 tgtagagcgg ggcgataggg cctgggtcgc ccccggcaaa gtgatagtcg taggccatga
     1261 caaggaggta gtcggtgtac tgggcgaggg cggcgtagtc gtaggcggcg gcccagccgg
     1321 tggtgacatc tctcgacttg ggggcgacgg ccatcgccac gagcttgcct ttggggtgca
     1381 gcctctcgta gagcttgccc atgaaggcgg tgaggtgggg cttgtcggag gggttgaggc
     1441 cctcgaagtc tatggtgatg ccgtcgtagt tgttggcggt gacgatgttg tcgatctggt
     1501 tgacgagcga ctgctgcttc aacgtgtcgg taagcacggg gctgaaggcg ttgtactggg
     1561 cggtgttttg cagcatgggg agggtcttgg ccccggcgtt gcggatgagg gcgctggcgg
     1621 ctgcctggtc tttgccgctc accgtgccgg tcatgttgac tgtgtagaac cagggggata
     1681 cgtagttgag gttgtggacg ttggcctgaa gggacactag ggagttggga ttgtaggtga
     1741 cgtaaaaggc ccagcgcacg ggcgcgctac cctgggcggc ctcggcggga cggctgccgt
     1801 tgagggaggc gagcaggagg gaggcggcca ggatggcgag tgctatgtag atgttgtggg
     1861 tgcgtaggcg catggctgtc tcctaaaggc gggaggcgag cttgtcataa agtgtcgggc
     1921 cgattatagg gcggggtggt ggtgtttgtc aaaattgcgg aaaagtgtgg gatgtgatgg
     1981 gtttgtcgcc tatgtaggag taacgatttt gttggaagga ggttgtaggg gaaggagggc
     2041 gcggggttgc cgctgttcgc ctggcatggt gctgtgcgat agccctgtgg ctaaagccac
     2101 gggatgagaa gtagcgaagt cccctgcggg gactaccctc atgtgagaga gtcccctaga
     2161 tagagtggta cgtggtgttg tgggtggctt ctaggtctct ggtcgcacct ttggggaggt
     2221 ggtgggcaaa acggttgaca gcgaaagctg catgggctaa tatatagtaa tactaactat
     2281 tggcgatgct aagcgaaagc accagatgaa acagttggag agaagcaatg gcgcatacaa
     2341 gaaggatagt ggaagaggcg gagcaggcac cggagcctgg gcacgggggg ctgttggagg
     2401 acgcggcggc ggagctgctg tcggtgctgc cccgcatgat acgggtgatc aagcactcgg
     2461 cgagatacgg ggagcaaagc gagcaggcgg ggccgctccg cgagcttggc gagtcgcagg
     2521 tgatggtgct tttcacgttg cggcaccagg gcacgcaact cacgagcgag cttgcccggc
     2581 gctacaacgt gaccaacccg acgatgacgc gcatcgtgga cgaactggtc aagaaggggc
     2641 tggtggtgcg gcagccggac acgaaggaca ggcggtgcat cttcctggaa ctgacggagg
     2701 aggggcggga gcttgcagcg gtggcacagg agcacggacg caaggcgctg gtagagtatt
     2761 tgcagccgct gaacaacgag gagctggccg acgtttcgcg ggcttttggg cacctgcgca
     2821 ggttgctacc ggaagcattg cgggacccgg cgagttgctc ggtgaggcgt atggaacagg
     2881 cgcacgtaga gggcgggcgc gggctgaact aggcggcttt agaagggtac acaggtattt
     2941 ttgattgtcg gaggaaccaa gagagtgaat agcgaggcta cacaagaggc cgaggggcag
     3001 gggaagcccg tcggagcggc aaccggtacg cagtacatgg gtgatgagac cccgcgcctc
     3061 ccgcgccggc agctagcggc gatcatgatt agcgtgctgc tcggcctgtt gctatcggcg
     3121 ctggaccaga caattgtcgg cccggccatg ccgaggatta tcggcgacct gaacggtttc
     3181 gaggcttatt cgtgggtgtt cacggtgtac ctgctcacgt ctacgatcac ggtgccgatt
     3241 ttcggcaagc tgtcggacat gtacgggcgc aaatggttct acgtgggtgg catattggtg
     3301 ttcctgctgg gttcggtgct ttcggggctg agcgccgaca tggcgcagtt gatcgcattc
     3361 cggggtctgc agggcatcgg tgccggtatc atgatggcta ctgcgtttgc gatcatagcc
     3421 gacctgatcc cgccctccga gcgtggcaag tggcaggggg cgttcggggc ggtgttcggc
     3481 atatcttcgg taattggccc gaccatcggg ggcttcctga cggataacct gaattggcgg
     3541 tgggtgttct atgtgaacat gccggtgggt gcgattgcca ttgccgcgct tgtgctgttg
     3601 tttccgaacg accgcaaggt ccagcacgcc cgcaggtcta tcgactggtg gggcgcgggc
     3661 actctagtgg cgggcctgac gccgctgctg ctggcgctgt cgctggtggg cacggctacc
     3721 agggtcgaac tgcctggtgt cggccttata acgagttggg gctgggattc gcccactatc
     3781 ctggctttgc tggcgatgtc ggtcgcgttc ctggtggcat ttgtgtacgt gcaggggcgg
     3841 gccaaggagc cgataatccc gctcgacctg ttcaagcata acatcttcac ggtgtcggtc
     3901 attacagtgt tcctgacggg cgtgggcatg ttcggcgcgg tgctctacat cccgctgttc
     3961 atacaggcgg tccagggcga ctcggctacc aatagcggca atagcgtgac gcccatgacg
     4021 ctggcgattg tgctctccag tattgtgacg gggcagatca tctcgcgcac agggaagtac
     4081 cgcatcatcg gtatcgtggg catggtcatg gtgaccgggg gcgc
//
LOCUS       DASRWZ010000374         1650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_511532, whole genome shotgun sequence.
ACCESSION   DASRWZ010000374 DASRWZ010000000
VERSION     DASRWZ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511532"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VFH60_05835"
     CDS             <1..1551
                     /locus_tag="VFH60_05835"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET6313338.1"
                     /translation="ALGTRHSALVYNAPMQTVPSPMVREAGPLVLAIDVGSSSLRTNL
                     FTAQGRQMAGCEAQLRYEVRVTPDGGVEIAPEHLLERIFEALDTTLALAGEHVGAIAG
                     VGMCSLVSNLLGIDALGHPTTPIYTWADTRCAAEAAHLRATLDERAIHERTGCYLHTS
                     YLPARLLWLRHALPEACSRTARWLTLGDWLFLRLFGEVAQSLSVASWSGLLDRHTLNW
                     DAAWLEYLELDREKLPPLVDAGAPFVGLRAEFAQRWPQLRAVPWFPCVGDGVTSNLGS
                     GGWAQDRVAVQVGTSGAMRVVVPSSVPRVPEGLWCYRATRDLSLLGGALSEGGNLLDW
                     LRETLNVGDWGASSQEAAMLPPDAHGLTLLPFVAGERSPGWDPAARATISGMSLGTRT
                     VDIMRAAQEAIMYRFGLIYDQLCGVVPAPGMVIASGGALLNVPGWIGMLSDVLGVPVT
                     ASGESEASSKGVAMLVLRTLGVIRDFSEVPTSLAETYRPNMENHEVYRRAMARQQAMY
                     EALRHGSR"
BASE COUNT          254 a          510 c          592 g          294 t
ORIGIN      
        1 gcactcggca ctcggcactc ggcactggta tataatgcgc ctatgcagac agtcccttcc
       61 ccaatggtgc gcgaggccgg gccgctggtg ctggcgattg acgtggggtc atcctccctg
      121 cgcaccaacc tgttcacggc gcaggggcgg cagatggcgg ggtgcgaggc ccaactgcgc
      181 tacgaggtac gcgtgacccc cgacggcggg gtggagatag cgccggagca cctgctggag
      241 cgcatcttcg aagccctcga cactacgctg gcgctggcgg gcgagcacgt aggggcgata
      301 gcgggcgtgg gtatgtgctc gcttgtgtct aacttgctgg gcatcgacgc gctggggcac
      361 cctactacgc ccatttacac ctgggcggat acgcggtgcg cggcggaggc agcccactta
      421 cgggctacgt tggacgagcg ggcgatacac gagcgcaccg gctgctacct gcacacttcc
      481 tacctgcctg ctcgcctgct gtggctgcgc catgccctgc ctgaagcttg cagccgcacc
      541 gcccgctggc ttacgctggg cgactggctt ttcttgcggc tgttcgggga agtggcgcag
      601 agcctctcgg tcgcctcctg gagcgggctg ctcgaccggc atactctcaa ctgggacgct
      661 gcctggttgg agtacctgga gcttgatagg gagaagctgc cgccgctggt ggacgcgggc
      721 gctcccttcg tggggctacg ggcggagttc gcgcaacgtt ggcctcagct acgggccgtg
      781 ccctggttcc cctgcgtggg cgacggggtg acgagcaacc tgggtagcgg tggctgggcg
      841 caggacaggg tggcggtgca ggtgggcacc agcggggcta tgcgggtggt tgtgccgagc
      901 agcgtgccgc gggtgccgga gggcttgtgg tgctaccggg ccaccaggga cctgtcgctg
      961 ctgggcgggg cgctctccga ggggggcaac ctgctcgact ggctgcgcga gaccctcaac
     1021 gtgggcgact ggggagcctc gtcccaggag gccgccatgc tgccgcccga cgctcacggc
     1081 ctgaccttgc tgcctttcgt ggcgggcgag cgcagccccg gctgggaccc ggccgcccgc
     1141 gctacaataa gcggcatgtc tctcggcacg cgcactgttg acataatgag agcggcacag
     1201 gaggcgatca tgtaccgctt cggcctgatt tacgaccagt tgtgtggtgt ggtgcctgcg
     1261 ccgggcatgg tgatagccag cggaggggcg ttgctcaatg tgccgggctg gattgggatg
     1321 ctctcggacg tgctgggcgt acccgtgacc gcctcgggcg agtcggaggc cagctccaag
     1381 ggggtagcca tgctcgtcct gcgcacactg ggcgtgatcc gcgacttcag cgaggtgccc
     1441 acctccctgg cggagactta ccgccccaac atggagaatc atgaggttta caggagggca
     1501 atggcgcgac agcaggcgat gtacgaggct ctgaggcacg ggtcacgcta ggggctatct
     1561 gtaaagagcc gctcatgtgg gaccaaaccc cccattgtca ttctgagcgc agcgaagaat
     1621 ctcgtcctac agcacccaaa taccaccatt
//
LOCUS       DASRWZ010000375         1696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_991112, whole genome shotgun sequence.
ACCESSION   DASRWZ010000375 DASRWZ010000000
VERSION     DASRWZ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991112"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFH60_05840"
     CDS             complement(<1..373)
                     /locus_tag="VFH60_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET6313339.1"
                     /translation="MPKDPTIRKVLVIGSGPIVIGQAAEFDYAGTQACIAVWEEGVEV
                     VLVNSNPATIQTDPDTADHVYIEPLTVESLRDIIAKERPDGLIATMGGQTALNLAVEL
                     ERAGVLSEYGVRTLGTSTATIR"
     gene            complement(366..1478)
                     /gene="carA"
                     /locus_tag="VFH60_05845"
     CDS             complement(366..1478)
                     /gene="carA"
                     /locus_tag="VFH60_05845"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET6313340.1"
                     /translation="MGDTLSGLLVMPDGTAVRGVGGGAPGLAVGELVFQTGMVGYQEA
                     LTDPSYAGQVLIFTYPLVGNYGVGDLSNQSARVHPRAVVAHQLVRGSGHRATTRDLDQ
                     MLRDQGIPALYGVDTRLLTRRVRTHGVIPTAVAVGPEAELPGVEELRLLAAALDYDHT
                     DFVAECTTPELVWYPPATENAPRVALVDYGSKRAILHHLRQMGAGVWLVPASMSASQI
                     MALRPDGVLLSNGPGDPARLDYAVATVRSLLQHGGVPIFGICLGHQLLALAAGGRTSK
                     MRFGHRGINQPVLELATGRVSITTQNHGYMVDPDTIPAGYVVTHTNLNDGSVEGIAHS
                     TLPVWGVQWHPEAHPGPSDTRQLFANFLGSMEVARA"
BASE COUNT          283 a          547 c          560 g          306 t
ORIGIN      
        1 tgcgtatcgt ggcggtggag gtgccgaggg tgcgcacccc gtattccgag agcacaccag
       61 cacgctccag ctccacggcg aggttgaggg ccgtctggcc gcccatcgtg gcgatgaggc
      121 cgtcgggccg ctctttcgct atgatgtcac gcaggctctc cacggtgagg ggttcgatgt
      181 acacatggtc ggccgtgtcg gggtcggtct gtatcgtggc ggggttggag ttcaccagca
      241 ccacctccac gccctcttcc cacacggcga tacaggcctg ggtgcccgcg tagtcgaact
      301 cggcggcctg ccctatcaca atcggccctg agcctattac aagcactttg cggatggttg
      361 ggtccttagg cacgggcaac ctccatcgac cccaggaaat tcgcgaagag ctgcctggta
      421 tcggacgggc ctgggtgcgc ctccgggtgc cactgcacgc cccacacagg cagcgtggag
      481 tgcgcgatgc cctccacgct gccatcgttc aggttggtgt gcgtgactac gtagcctgcc
      541 ggtatcgtat cggggtccac catatagccg tggttctggg tggtgattga cacgcgcccg
      601 gtggctagct ccagtactgg ctggttgatg ccccggtgtc cgaagcgcat cttggaggtg
      661 cgtccccctg ccgctaacgc cagcagttgg tgccccaggc agatgccgaa gatgggtaca
      721 ccgccatgtt ggagcagaga gcgcacggtc gccaccgcgt agtcgagcct agccgggtcg
      781 ccaggcccgt tcgacaggag cacaccgtcg ggccgcagcg ccattatctg gctagcgctc
      841 atgctcgcag gcaccagcca gacgcccgcg cccatctgcc taaggtggtg cagtatcgcc
      901 cgcttgctgc cgtagtccac caacgcgacg cggggagcat tctcagtggc cggggggtac
      961 cagaccaact ccggggtggt gcactctgcc acgaagtcgg tgtggtcgta gtcgagtgcg
     1021 gcggcgagca ggcgaagctc ctccacgccg ggtagctcgg cttcaggtcc gaccgccaca
     1081 gccgtcggta tcacgccgtg ggtgcgcacc ctgcgcgtga gcaggcgcgt gtctacaccg
     1141 tagagcgcgg gtataccctg gtcgcgcagc atttggtcga ggtcgcgggt agtagccctg
     1201 tggccgctgc cccgcacaag ctggtgggcg acgacggccc tggggtgcac gcgggcgctc
     1261 tggttggaga ggtcgccaac gccgtagttg cctacgagcg gataggtgaa gatgagcacc
     1321 tgtccggcgt aagaggggtc ggtgagcgct tcctggtagc cgaccatgcc ggtctggaac
     1381 accaactcgc caacggccag ccccggcgcg cccccgccca cgccccgcac cgccgtgccg
     1441 tccggcatca cgagcagccc cgacagagta tcacccatcc cgtgctgtac ctccaacggg
     1501 cacccttgcc tgcggcctct cgacgttgag agctttgatt tgttcggaag attcgcgtgc
     1561 aaaggctgcg gaacgtgcgt gctccgctcg ccaatggaga ttttagcaag aactgacgcg
     1621 tgggggtaga gttgtgcacc gcaaacccga ctaatgtgtc gctgccgcta ccagaaacca
     1681 ttgcaagaac agagta
//
LOCUS       DASRWZ010000376         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_237074, whole genome shotgun sequence.
ACCESSION   DASRWZ010000376 DASRWZ010000000
VERSION     DASRWZ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237074"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VFH60_05850"
     CDS             complement(<1..1539)
                     /locus_tag="VFH60_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET6313341.1"
                     /translation="MSNFSYLNFDVHIQRATDGGYRVVVEAPSGETIRAPFDLPFSEL
                     ELENFMLKAGPSRRGVRRIETSEVEAAKRFGEKLYASLFTADVRSAMQSSIDQARQRG
                     ESGEERVGVRLRLRLVDVPELAGVPWEYIYNPGLNRFLALSTETPLVRYLDLPERIRP
                     LKVVQPLRVLVMLPSPSDYPELDVRQEWLNLSTTLAGLQQAGLVQLDLLEVSTLTGLQ
                     RQLRRDAYHIVHVIGHGAFDRNVADGLLIFEDEDGRGRPVSGQDLAMLLHDHASLRLA
                     VLNACEGALSAADDPFAGVAQSLVQGGLPAVIAMQFEITDRAAIVFAHEFYRAISDGY
                     PVDAALTEARKVLLTESRGNGLEWGTPVLYMRSPDGRIFNLEKRQHTTDAPAIAAPAS
                     TPSPGPAPAPAATHASRSGATEAAASPTATGPQAQPRPPAAAPASTSAVTRSGEAPLA
                     RGVEKPPTGKGNTRLLAAVLVITALVLAAGAGLFFLTRSGGNSAPTSPGQVPAEASAH
                     NDRGL"
BASE COUNT          298 a          560 c          630 g          324 t
ORIGIN      
        1 tagcccccgg tcgttgtgcg cgctcgcctc ggcgggcacc tgccccggag aagttggtgc
       61 tgaattcccc ccacttctag taaggaagaa cagccccgcg cccgcagcaa gcaccagggc
      121 cgtgatgaca agcacggcgg caagcaggcg tgtgtttccc ttgcccgtag gaggcttttc
      181 taccccacgc gccaggggag cttcgccact tctggtgacg gccgacgtgc ttgcaggcgc
      241 agcagccggg ggccggggtt gtgcctgcgg tccggtggcg gttgggctag cggctgcctc
      301 tgttgctcct gagcggctgg catgcgtggc ggctggggct ggcgcggggc cgggggaagg
      361 tgtcgaagcg ggagcggcga ttgcgggggc gtccgtggtg tgctggcgtt tctccaggtt
      421 gaagatgcgg ccgtcgggcg agcgcatgta cagcaccggg gtgccccatt ccaggccgtt
      481 gcccctgctt tcggtgagca gcaccttgcg cgcctcggtg agcgccgcgt ccaccgggta
      541 gccgtccgag atggcgcggt agaactcgtg ggcgaagacg atggcggcgc ggtcggtgat
      601 ctcgaactgc atggcaatca ccgcaggcag gcccccttgc accaggctct gcgcgacacc
      661 cgcgaacggg tcgtctgctg ccgagagcgc gccctcgcag gcgttgagca ccgcgaggcg
      721 caacgatgcg tggtcgtgca gcagcatcgc caagtcctgc ccgctcaccg ggcggccccg
      781 gccgtcctcg tcctcgaaga tgagcaggcc atcggccacg ttgcggtcga aggccccgtg
      841 cccgatgacg tggactatgt ggtacgcgtc ccggcgcaac tgcctttgta agccggtgag
      901 tgtggacacc tccaggaggt ctagctgcac gaggcccgcc tgttgcaagc ccgcaagggt
      961 ggtgctcagg ttgagccatt cctggcgcac gtctagctcg ggatagtcgc tcggtgaggg
     1021 cagcatgacc agcacgcgca ggggttggac caccttcagg gggcggatgc gttcgggcag
     1081 gtcgaggtag cgcaccaggg gcgtctcggt ggaaagggcg aggaagcggt tcaggccggg
     1141 gttgtagata tactcccacg gcaccccggc caactccggc acatcgacca ggcgcagtcg
     1201 cagtcgcacc cccacgcgct cctcgcccga ttcgccacgc tgtcgggcct ggtctatgct
     1261 gctctgcatg gcgctacgca cgtcggcggt gaacagggac gcgtaaagct tctccccgaa
     1321 acgcttggcg gcctccacct cggaggtctc gattcgacgc acgccccgcc gcgagggacc
     1381 tgccttgagc atgaagttct caagctccag ctcggagaat ggcaggtcga acggggcgcg
     1441 aatggtctcg cccgaagggg cctctacgac tacgcggtag ccgccgtcag tggcccgttg
     1501 tatgtgcacg tcgaagttca ggtatgagaa gttagacatg actcctcgga ggtgtgtgga
     1561 ggttggatat tttagttcgt attgtagcag aaggctacat gggtattcaa caagcagcct
     1621 taggcgtatc cctgtggtga cgccctgcca ctaaagtggc gggcttacaa gaggcaaagt
     1681 cccctgcggg gactaagaga cctgagggta gtctccgtag gagacttcgt ctctggtcag
     1741 cccgccaatt cattggcagg gcgaaggcca cagggctatc gtaaggcctc ccctcacaca
     1801 ggggcttccc ac
//
LOCUS       DASRWZ010000377         4784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1128496, whole genome shotgun sequence.
ACCESSION   DASRWZ010000377 DASRWZ010000000
VERSION     DASRWZ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128496"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2270
                     /locus_tag="VFH60_05855"
     CDS             <1..2270
                     /locus_tag="VFH60_05855"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HET6313342.1"
                     /translation="LIMNGCDHFLPQPATQEVLDEANRRQRTIKLRQGSLEEYVGLVM
                     EARPELEVKSGEWRHNRPSRITPGVLSARMYLKQADFRASTLLERGAEPLQALVSLFG
                     GKHDTGLMGTAWRYLLQNHPHDSICGCSIDAVHRDCEARFRWAEEIAGDLVERGAFAL
                     SKRVKATSGPGLVVFNTLARPRTELAHMRLHFLEPGTGFYLTDPAGGRIPHQEASRRP
                     MKVEWDPQRESFYCSGRIYPATVVSAVDRAAIGGRWQRWIGEEVEVLFPANLPAGGYT
                     TVSVVPQQDGPTNGVGKAPASQEQTPGVTSGANWLENELVRVEVGDNGSFTLTDKRSG
                     ERYGPLNVFRSEADRGDEYSFCPAEADEPVDSLGCTASMGMVEAGPLRATIEVSLAMQ
                     VPAALAEDRTLRVDERVTVPIRSRISLSAGQRRVQVRTEVENTARDHRLRATFMTGVQ
                     TDLADAQGQFEVMRRPVDLPPEERARVPEFDEEQEVSYHPQRAFVDVSDGSRGLAVLN
                     RGLPEYEATPSEEGVWLGVTLLRCVGWLSRDDLSTRYKHAGPPLETPEAQCLGKYAFE
                     YAVVAHTGDWLEGGIPAEAESYVSPIFSAPVHGKAPMQQTGVLVEADDATDAVGESGE
                     VTIDEHSATRMIEDTSVLANPGAADSNAAGLLPSEASFYSIAPFELLFSACKRSEEGD
                     ALILRVYNTAPYPVEGTLALGIPGTVRLANLAERELSEPLMPGAGGGMGASVYRFAAN
                     KHEIVTLSIRSENQP"
     gene            2267..2935
                     /locus_tag="VFH60_05860"
     CDS             2267..2935
                     /locus_tag="VFH60_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HET6313343.1"
                     /translation="MTANDTSRGAGETTSADNIQARELVRGHFLESAEVKRQMAEVLA
                     GPAVEAAARIASALHAGNKVLVFGNGGSAADAQHFAGEMVGRFKLNGRAALPVLALTT
                     DSTVLTCVANDFSYEEVFSRQVEAFAQPGDVVVGISTSGKSPNVVRALRAAREQGAIA
                     IALLGGDGGTAAAEADLPIIVASKDAARIQECHITLIHAISELIEAEIMRAADNARTD
                     GGQS"
     gene            2932..4698
                     /locus_tag="VFH60_05865"
     CDS             2932..4698
                     /locus_tag="VFH60_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313344.1"
                     /translation="MRIEEMAEAILIEGDGYTLRLSREQPTAELELNGRRISTLNMAS
                     ALDAMDGRDEASAMEAPTLAGDDAGATVTWRGRSDRWQEKVVELRAWEEGFSYGYSVV
                     GEGQIDRAHLLRTRQTPSPSYGARLFNPEPNSGIVRYTRERCDPVRNCHVCFPEKFQS
                     PLKQEVPPDFMTITVGRDKTYHDGNWFFTPAPFCYAVEGEGNWLAMGVAAAAGAWQFS
                     DMYYPGGGFGFSLVYDGHEAVRGSWESPRLVCLTAPDEYEAIARYCDRLREQGLAPTQ
                     GRGPAQDWWRQPIFCGWGEQVSQEVHLGEPKAQAGCRQESYERWLATLDNHGLDPGTV
                     VIDDKWQLSWGMNDVDTDKWPDMQGFIAGQHERGRRVLLWLKAWEPEGVPPEECIVSE
                     EGVPVSVDPGNPAFEARLSEQVRYMLRELDADGFKIDFTHLIPRGAGIRSAGGKWGLE
                     LMRQWLSIVSDAAREVKPDALVMTHTANPYLADVSDMLRLNDVVGLPDIHASIIPDMR
                     HRARIARMASPYWLLDSDNWPCSSRPQWREYVQAQASGEFGIPSLYHAERLGWGAVDE
                     PLAEEDYEAVRQAWKEYRRNSA"
BASE COUNT          897 a         1479 c         1682 g          726 t
ORIGIN      
        1 tgctcatcat gaacggctgc gatcactttc tgccccagcc cgccacccag gaggtgctgg
       61 acgaggccaa ccgccgccag cgcacgatca aactgcggca ggggtcgctt gaggagtacg
      121 tggggctggt catggaggcg cggcccgaac tggaggtgaa gagcggggag tggcggcaca
      181 accggccctc gcgcatcacg ccgggcgtgc tgtcagcccg catgtacctc aagcaggccg
      241 atttccgggc gtctaccctc ctcgaacggg gggccgagcc tttacaggcg ctggtatcgc
      301 tgtttggggg caagcacgac acggggctta tgggcaccgc ctggcgctac ctgctgcaaa
      361 accacccgca cgactccatt tgcgggtgca gcatcgacgc ggtgcacagg gactgcgaag
      421 cgcggttccg ctgggccgag gagatagcgg gggacctggt ggagcgcggg gcgtttgcgc
      481 tgtcgaagcg tgtaaaggca acatccggcc ccggcctggt cgtgttcaac acgctggccc
      541 gcccccgcac cgaactggcc cacatgcgcc tgcacttcct ggagcctggc accgggttct
      601 acctcaccga ccccgccggg gggcgcatac cacaccagga ggcgagcagg cggccgatga
      661 aggtagaatg ggacccgcag cgcgaaagct tctactgttc ggggcgcatc taccccgcta
      721 ccgtcgtgtc ggcggtggac agggctgcta taggcgggcg gtggcagcgg tggatcgggg
      781 aggaggtgga ggtactgttc cccgctaacc tcccggcggg cggctacacc actgtgagcg
      841 tggtcccgca acaggatggg ccaacaaacg gcgtgggtaa ggccccggca agccaggagc
      901 aaacccccgg tgtcacgtcg ggcgctaact ggttggaaaa cgagctggtg cgggtggagg
      961 taggcgacaa cggctcgttt acgctcacag acaagcgttc cggcgagcgg tacgggccgc
     1021 tgaacgtctt ccgcagcgag gccgaccggg gcgacgagta tagcttctgc cccgccgagg
     1081 ccgatgagcc ggtggatagc ctgggctgca cggccagcat gggcatggtc gaagcagggc
     1141 cgttgcgagc cacaatagag gtgagcctgg cgatgcaggt gcccgccgcc cttgccgagg
     1201 accgcacgct gcgcgtggac gagcgtgtga cggtgcccat tcgcagccgc atatctctca
     1261 gcgcggggca gcgccgggtg caagtgcgta ccgaggtgga gaacacggcc cgcgaccaca
     1321 ggctgcgcgc caccttcatg accggggtgc aaacggactt ggcggacgca cagggccagt
     1381 tcgaggtgat gcggcggccc gtggacttac cgccggagga gcgggcgcgg gtgccggagt
     1441 tcgacgagga gcaggaggtc agctaccacc cgcaacgcgc gttcgtggac gtgtcggatg
     1501 gtagccgggg actggccgtg ctcaacaggg ggctgccgga gtacgaggca acgccctccg
     1561 aagaaggtgt gtggctgggc gtgacgctgc tacggtgcgt gggctggctg agccgcgacg
     1621 acctttctac ccgctacaag cacgcaggcc ctcccctcga aacgcccgaa gcgcagtgcc
     1681 tcggcaaata cgccttcgag tacgcagtcg tagcccacac cggcgactgg ctggaaggtg
     1741 gcattcccgc cgaggccgag tcctacgtaa gccccatctt cagcgccccg gtccacggca
     1801 aggcccctat gcagcagacc ggcgtgctgg tagaggcaga cgatgctacg gatgcggtgg
     1861 gggagtcagg cgaggtcacc atcgacgagc acagcgccac gcgcatgatt gaggacacgt
     1921 cagtcctggc caaccccggc gcggcggata gcaatgcggc cgggctactg ccttccgagg
     1981 ccagcttcta cagcatcgca ccgttcgagc tattgttcag cgcgtgcaag cggagcgagg
     2041 agggggatgc actcatcctg cgggtctaca acaccgcgcc ctaccctgtg gagggcacac
     2101 tggcgcttgg cattcccggc acggtgcgcc tggcgaacct cgcagagcgg gaactatcag
     2161 agcctcttat gccgggggcc gggggaggca tgggagctag cgtataccgt tttgccgcga
     2221 acaagcacga aatcgtgact ctttccatca ggagcgaaaa tcagccttga cagctaacga
     2281 cacatcgaga ggcgcaggag aaacaacgag cgcggacaac attcaggcgc gggagttagt
     2341 gcgcgggcac ttcctggaga gcgccgaggt caagcggcag atggccgagg ttctggcggg
     2401 gcctgcggtg gaggctgcgg cgcgtatcgc atcggccctg cacgcgggga ataaggtgct
     2461 ggtgttcggc aacggcgggt cggctgccga cgcgcagcac ttcgcggggg agatggtggg
     2521 gcggttcaag ctcaacggca gggcggccct ccccgtgctg gccctcacca ccgattcgac
     2581 ggtgctcacc tgcgtagcca acgacttctc atacgaggag gtgttcagca ggcaggtgga
     2641 ggcgttcgcc cagccgggcg acgtggtggt gggcatcagc accagcggca aatcgcccaa
     2701 cgtggtgagg gccttgcgcg ccgcccgcga gcagggggcc attgctatcg ccctgctggg
     2761 aggggatgga ggcaccgccg cggctgaggc cgacctgcct atcatagtgg cctccaagga
     2821 tgccgcccgc atacaggaat gccacatcac cctcatccac gccattagcg agttgataga
     2881 ggccgaaatc atgcgcgcgg ctgacaatgc ccgcacggac ggaggccaga gttgagaatc
     2941 gaggagatgg cagaggctat ccttatcgag ggggatggct atacgctacg gctgtcgcgg
     3001 gagcaaccta ccgccgagct ggagctaaac ggcaggcgta tctctaccct gaacatggcc
     3061 tccgcgctgg atgcgatgga cgggcgcgac gaggcgagcg cgatggaggc ccctaccctc
     3121 gccggcgacg acgctggagc cacggtcacc tggcggggca gaagcgaccg ctggcaggag
     3181 aaggtcgtgg agttgcgggc ctgggaggaa ggcttctcct acggctacag cgtagtaggc
     3241 gaaggccaga tagaccgcgc gcacctgcta cgcactcgcc aaacgccctc gccctcgtac
     3301 ggcgcgcgcc tgttcaaccc ggagccgaac agcggcatcg tgcgctacac gagggagcgg
     3361 tgcgaccccg ttcgtaactg ccacgtctgc tttcccgaga agttccagtc cccactaaaa
     3421 caagaggtcc cgcccgattt catgaccatc accgtagggc gcgacaagac ctaccacgac
     3481 ggcaactggt tcttcacccc ggccccgttc tgttatgcgg tggaggggga gggcaactgg
     3541 ctggcgatgg gtgtggccgc ggcggctggg gcctggcagt tcagcgacat gtactacccc
     3601 ggcgggggct tcggcttcag cctggtgtac gacgggcacg aggcggtgcg cggctcctgg
     3661 gaaagccccc ggctggtctg cctcacggcc cccgatgagt acgaggcgat agcgcggtac
     3721 tgcgaccggc tgcgagagca ggggctggca ccaacgcagg gcagaggtcc ggcgcaggac
     3781 tggtggcggc agcctatctt ctgcggttgg ggcgagcagg tatcgcagga ggtccacctg
     3841 ggcgagccga aggcccaggc gggttgcagg caggagagct acgaacgctg gctggccacg
     3901 ctcgataacc acgggttaga ccccggcaca gtcgttatag acgacaagtg gcagctatcg
     3961 tggggcatga acgacgtaga tacggacaag tggcccgaca tgcagggctt catcgcgggg
     4021 cagcacgaac ggggcaggcg cgtgctcctg tggctgaaag cttgggagcc tgaaggtgtg
     4081 ccgccggagg agtgcatcgt cagcgaagag ggcgtgcccg tgtcggtgga ccccggcaac
     4141 cctgcctttg aagcaaggct atccgagcag gtacgttaca tgctgcggga gctggacgcg
     4201 gacggcttca agatagactt cacccacctg atacccaggg gtgccggtat ccgctcggcc
     4261 ggcggcaagt gggggctgga gctgatgcgg cagtggctct ctatagtcag cgatgcggcc
     4321 agggaggtca agcccgacgc cctggtcatg acccacaccg ccaacccgta cctggcggac
     4381 gtatcggaca tgctcaggct gaacgacgtg gtgggcctcc ccgacatcca cgccagcatc
     4441 atacccgaca tgcggcacag ggcgcgtatc gcccgcatgg cctcgcctta ctggctgctc
     4501 gactcggaca actggccctg ctccagccgc cctcagtggc gcgagtacgt gcaggcacag
     4561 gcaagcggcg agttcggcat cccctccctc taccacgccg agcggctggg ctggggtgcc
     4621 gtcgatgagc cgctagcgga ggaagattac gaggcggtcc gccaggcatg gaaggagtat
     4681 cgaaggaaca gcgcgtaaca cgtaacgcgt aacacgtaac gcggctcaag agactcggta
     4741 ccagataagc cactctatcg ccttcgctac cttacttacg cgtt
//
LOCUS       DASRWZ010000378         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_991326, whole genome shotgun sequence.
ACCESSION   DASRWZ010000378 DASRWZ010000000
VERSION     DASRWZ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991326"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..2576)
                     /gene="murC"
                     /locus_tag="VFH60_05870"
     CDS             complement(117..2576)
                     /gene="murC"
                     /locus_tag="VFH60_05870"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET6313345.1"
                     /translation="MDLSSQRWHFIGIGGAGMSALAEALLDLGATVSGSDLSASDVTR
                     ALEARGARVSIGHHAENLGDADRVVYTGAVPADNPEVLEAQRRGLPLVRRAELLGQLM
                     DLRRGVAVAGTHGKTTTSSMLAWVLARAGRDPSYMVGGTIRGLGPGGHWGSGPELVAE
                     ADEYDRSFLQLRPEVAIITNIDSDHLEYYGTNDAIYAAFGEFAANVRPGGLLIVCADD
                     PASMQLASDLQASSASFRLQVYGAAPDALWRPDGIMPNEIGGSDYVALRNGQVAARVS
                     LRVPGQHNVLNSLAVMAACVELGLSPGEVARWLEEFEGAGRRFEVKGEVGGITVVDDY
                     AHHPTEITATLRAARQRYPQRRLMVLFQPHTYTRTRDFLGDFATALDLADRAYVTEIY
                     ASRERDTLGMSGRLVVERMAAHAEFVPTLKEAVQVLLDDAAPGDVVITMGAGDVWQAG
                     EELLAALSQGEDDEGIEPVEVERHIPEAPRLDLQPPGELTLEQAQPPQGVGPKPRATV
                     SFRGDPAVEMEAATGLKVLRDEPMSKHNSLRVGGPASFFVAAGNKAQLIAAVLFARER
                     GLPYLVLGNGTNILVGDYGIDGLVIHNKAQDISHELLDEGRSLWHVESGVLFSRLARI
                     TCEQGWTGLEWSNSVPGSVGGGVVSNAGAHGKDLHDDLVSVEVLTREGKVEVWPADTL
                     ELGYRTSRFKAHGSRALSNPEVILSAGITLYRDANHECEARMREYLAERQAKQPQGKS
                     AGSTFKNPPGNSAGYLIEQVGLKGFRHGQAQFSPKHANFMMNLGGATTADVLHLMRLA
                     QDRVREQFGIELEPEIEYIGE"
BASE COUNT          423 a          901 c          862 g          507 t
ORIGIN      
        1 agaaggacca gggtacgcca atatgtaccc tggtcctttt tactaccctt ataaaccatc
       61 ctaatctttg cgcgttcttt gcgtcctttg cgtctttgcg ttttcgtact tctaccctat
      121 tccccgatgt actcaatttc gggttccagc tcgatgccga actgctcgcg gacgcggtcc
      181 tgggcgaggc gcatgaggtg gagcacgtcg gcggtggtgg caccgccgag gttcatcatg
      241 aagttggcgt gcttggggga gaactgggcc tggccgtggc ggaagccttt caggcccacc
      301 tgctcgatga ggtagcccgc ggagttgccg ggggggttct tgaaggtgga gcctgcgctt
      361 ttgccctggg gctgtttggc ctggcgctcc gcaaggtact cgcgcatcct ggcctcgcat
      421 tcgtggtttg cgtcacggta gagggtgatg ccggcgctga gtatcacttc ggggttggac
      481 agcgcgcggc tgccgtgggc cttgaagcgg ctggtgcggt agcccaattc gagcgtgtcg
      541 gcgggccaga cctctacctt gccttcgcgg gtgagcactt ccacgctcac gaggtcgtcg
      601 tgcaggtctt tgccgtgcgc gccggcgttg ctcaccacgc cgccgcccac gctgcccggc
      661 acgctgttgg accactccag ccccgtccag ccctgctcgc acgttatcct ggcgaggcgg
      721 ctgaacagca cccccgactc gacgtgccag aggctgcggc cctcgtccag tagctcatgg
      781 gatatgtcct gcgccttgtt atggatcacc aggccgtcta tgccgtagtc gcccaccagg
      841 atgttggtgc cgttgccgag caccaggtaa ggcagtccgc gctccctggc gaacagcacc
      901 gccgcgatga gttgcgcctt gttgccggcg gccacgaaga aggaggcggg gcctcccacg
      961 cgcaaggagt tgtgcttgct catcggctcg tcgcgcagca ccttcagccc ggtagccgcc
     1021 tccatctcca ctgccgggtc ccctctgaag gacacggttg cacgtggctt cggccctacc
     1081 ccctgtggcg gttgcgcctg ctccagcgta agctctcccg gcggctgaag gtccaacctg
     1141 ggagcttcgg ggatatgtcg ttcgacttct acaggctcaa tcccttcgtc gtcctcgcct
     1201 tgcgacaggg cggctaacag ttcttccccg gcctgccaca catcgcccgc gcccatcgta
     1261 atcacaacgt cgcccggggc cgcgtcgtcc agcagcacct gcacggcttc cttgagggtg
     1321 ggcacgaact cggcgtgcgc ggccatgcgc tccacgacca agcggcccga catgcccagg
     1381 gtgtcgcgct cgcgggaggc gtagatttcg gttacgtagg cccggtcggc aaggtcgagg
     1441 gcggtcgcga aatcgccgag gaagtcgcgg gtgcgggtgt aggtgtgcgg ctggaaaagc
     1501 accatgagac gccgctgcgg gtatcgctgc cgggccgctc ggagggtggc cgttatctcg
     1561 gtcgggtggt gcgcgtagtc gtccacgacg gtgatgccac caacctcccc tttcacctcg
     1621 aaccggcggc ccgcgccttc aaactcctca agccagcggg caacctcccc aggcgagaga
     1681 cccagttcca cgcacgccgc catgaccgcc agcgagttga gcacgttatg ctgccccggc
     1741 acccgcaaag acacccgcgc cgctacctgc ccgttccgca gggcgacgta gtcgctgccc
     1801 cctatctcgt tgggcattat accgtcgggc cgccagagcg cgtcaggggc ggcgccgtag
     1861 acctgcaagc ggaaggacgc ggagcttgcc tgcaagtcgg aagcaagctg catggaagcc
     1921 gggtcgtcgg cgcacacgat tagcaggccg cccggacgta cgttggccgc gaactccccg
     1981 aaggccgcgt atatggcgtc gttggtgccg tagtactcaa gatggtcgct gtcgatattg
     2041 gtgatgatag caacttcggg gcgcagttgc aggaaggagc ggtcgtactc gtcggcctcg
     2101 gccacaagct ccggcccgct gccccagtgc ccgcccggcc caagtccccg tatcgtgccg
     2161 cccaccatgt atgaagggtc gcgcccggca cgtgcgagca cccaggccag catggaggag
     2221 gtagtggtct tgccgtgcgt gcccgccacc gccaccccgc gccgcaggtc catcaactgc
     2281 cccaacagct cggcccggcg gacgagcggc aagccccgcc gttgcgcctc cagcacctcc
     2341 gggttatcgg cgggcaccgc gccggtatac accacccggt ccgcgtctcc caggttctcc
     2401 gcatgatgcc ctatcgagac ccgcgctccc ctggcctcaa gggcgcgcgt aacgtcggaa
     2461 gccgagaggt cggagcctga gaccgtagcg ccgaggtcga gcaacgcctc cgccagggcg
     2521 ctcatgcccg ccccgcctat tccgatgaag tgccagcgtt gggaagatag gtccattggt
     2581 tccgttctgg acataaaggg ggtatttcct gcgtgagaat tgtgtgcctg tgctttgctc
     2641 ccgcccgtat aaagagcgcg aattgcggtt gacgcaagta cagaaccaga gta
//
LOCUS       DASRWZ010000379         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_237441, whole genome shotgun sequence.
ACCESSION   DASRWZ010000379 DASRWZ010000000
VERSION     DASRWZ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237441"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1002)
                     /locus_tag="VFH60_05875"
     CDS             complement(76..1002)
                     /locus_tag="VFH60_05875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6313346.1"
                     /translation="MPTKTTKVSDQPGEKPKPLAREELFDQMVVETYSHEAAEFDEFG
                     HGLAEKSDRKLVEVISPRLGDHVLDVATGTGNLALALADRAGATGKVVGIDLAEGMLE
                     FAERKARARKVKNVEYKMMDARHLEFEDNTFDLVTCGLAVFYFPDIPGTLAEMCRVLK
                     PGGRLVISSADPETAFAPLSQPYMARLRKVSEELDLHPPEYSEAARLTRTADGLEQLL
                     RGAGLQRVHAREESIPVHFTSPGDWWKYGRGSTWGDILLQELPPEKRAEFEEKHLKEV
                     RKYFSDEGVKTATPILIAIGQKPYGRQPVPDM"
     gene            complement(1066..2985)
                     /locus_tag="VFH60_05880"
                     /pseudo
     CDS             complement(1066..2985)
                     /locus_tag="VFH60_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
BASE COUNT          495 a          991 c          965 g          623 t
ORIGIN      
        1 ctttaaccct taactactct ttgcgcgttc tttgcgttct ttgcgtcttc gtcccctgac
       61 ttactacccc agccgttaca tgtcggggac gggctggcgg ccgtaaggct tctgcccgat
      121 ggctatgagt atcggggtag cggtctttac gccctcgtcc gagaagtact tcctcacttc
      181 cttcaggtgc ttctcctcga actcggcacg cttctccggc ggaagctcct ggagcagaat
      241 atccccccag gtgctgcctc ttccgtactt ccaccagtcg cccggcgagg taaagtgcac
      301 cgggatcgac tcctcacgcg cgtgcacgcg ttgcagcccc gccccgcgca aaagttgctc
      361 caatccgtct gcggtgcggg tcagcctcgc cgcctcggaa tactcaggcg ggtgaaggtc
      421 cagctcctcc gagactttgc gcagccgcgc catatagggc tggctcagcg gcgcgaaggc
      481 cgtttccggg tcggcggacg aaatcaccag gcggccccca ggcttgagca cgcgacacat
      541 ctcggccaac gtgccgggga tgtcagggaa gtaaaacacc gccaggccac acgtcaccag
      601 gtcgaaggtg ttgtcctcga actcaaggtg gcgggcgtcc atcatcttgt actcgacgtt
      661 cttgaccttg cgggcgcgag ctttgcgctc ggcgaactcc agcatgccct cggccaggtc
      721 gatgcccact accttgccgg ttgcccccgc cctatcggcg agcgcgagcg ccaggttgcc
      781 ggtgccggtc gctacgtcca ggacgtggtc tcccagcctg ggggagatca cttccaccag
      841 cttgcggtcc gacttttcgg ccaggccgtg cccgaactcg tcaaactccg ctgcctcgtg
      901 gctgtaggtc tccacgacca tctgatcgaa cagctcctcg cgggccagcg gcttgggctt
      961 ctcgcccggc tggtccgaga ccttggtggt cttggtgggc atggcggtgg tggtgccatg
     1021 ggcggcagcc gtcgcctcgc gctcgatgtt ggtggcgtcg ccgtgctggc ggatgtagtt
     1081 cagcaggccg ccttccacca tgatgttcac ctggcgcagg gtcaggttgt gcttcaacag
     1141 gtacttgctt cccttggtga cgttctccac ctctatcgtc tcggcacccg acaacagcac
     1201 ctcgcgggcg ttcgccatgc gaagctcgtc gccttccgag atgtcggcgt agtccagcgg
     1261 gtcgatgaac accaggggga ggatgccgaa gttgatcagg ttgtcgcggt ggatgcgggc
     1321 gaagctcttg accagcacgg ctctgacgcc caggaacatg ggggcaagcg ccgcgtgctc
     1381 ccggctagaa ccctggccgt agttgctccc gcccacaacg aaaccgcccc ctgcctcctt
     1441 ggcgcgggtg gcgaacgaag ggtccacctt ctcgaacacg tactccgaga tgccggggat
     1501 gttcgagcgc aggggcagga tcttggcacc ggcgggcatg atgtggtccg tggtgatgtt
     1561 gtcgcccacc ttgagcagcg tcttgccttc caggtcggcg ggcatcggct tgttgatagg
     1621 cagcggcttg atgttcgggc cgcgcactac ctccaccgat tcggggtcgt cggcgggaga
     1681 cactatcatg ttgtcctcga ccaggaactt gggcggcact tccacctcaa gcctgccgcc
     1741 gagcgtgcgg gggtcggtga taacgcctgt aagggccgag gccgcgcagg tctcaggcga
     1801 ggcgagatag acctgggcgt tgggggtgcc ggagcgccct tcgaagttgc ggttgaagga
     1861 gcggatggac accgcgcccg atgagggggc ctggcccatg ccgatgcacg gaccgcaagc
     1921 cgactcaagt atgcgcgcgc ccgactcaat caggtcgccc aaagcgccgg agcgggctat
     1981 catggtgtac acctgccgcg agccgggggt gacgccgaac gagagggagg cgtgcaccgt
     2041 ctgccccttg agcacgcccg ccaccgtttg caggtccacg aatgacgagt tggtgcagga
     2101 gcctaccagc acctgcgaaa cgggcgtgcc cgctacctcg cgcaccggca ccacgctgtc
     2161 cgggctgtgg gggcgcgaaa tcagcggctc aagctcgtcc agcactatct ctatgatgcc
     2221 gtcgtactcg gcgtctttgt cggggccaag ctctacccag tccttgtcgc ggccctgggc
     2281 cttcatgtat tccagcgtgc gcttgtccga ggggaagata gaggtggtgg cccctagctc
     2341 cgcgcccatg ttggtgatcg tgccgcgctc cggcaccgac aggtcggaca cgccaggccc
     2401 gccgtactcg actatcttac caacgccgcc cttcacggtg aggcggcgca gcagctcgaa
     2461 aatgatgtcc ttggcggcca cccacggctg ccgcttgcct atcaggcgca ccagccagac
     2521 ttccggcata gccatgtaga aggggccgcc acccatagcg accgctacgt ccaggccgcc
     2581 cgccccaatc gagatcatgc ccaggccgcc gcaggtgggg gtgtggctgt ccgagccgag
     2641 caacgtcttg ccggggatag cgaaccgctc gaggtgcacc tggtggcaaa taccgttgcc
     2701 ggggcgcgag aaaaagatgc cgtacttggc ggcgatggac tggaggaacc tgtggtcgtc
     2761 cgcgttctca aagccggtct gcagcatgtt gtgatcgacg tagctcaccg acacgtcggt
     2821 gcgcacccgt ggtatgccca tcgcctcaaa ttgcaggtag gacatggtgc cggtggcgtc
     2881 ctgtgtaagt gtctggtcga tcctgatggc tacctcttta ccgggcttca tctcccccga
     2941 tacgaggtgc gcctcgatga tcttttgtac caggtttctt ggcattacct tcctccgggt
     3001 gactgcgttt gtctgtcttc tatgtttgcg ttctaatatt cgctctatcc gttaattata
     3061 tcacacagcg tcct
//
LOCUS       DASRWZ010000380         7483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1265989, whole genome shotgun sequence.
ACCESSION   DASRWZ010000380 DASRWZ010000000
VERSION     DASRWZ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265989"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFH60_05885"
     CDS             complement(<1..846)
                     /locus_tag="VFH60_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005548569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET6313347.1"
                     /translation="MRVLVTGITGFAGSHMAEYLAADHADVEIFGVYRRRSRLEHMQD
                     TDLQINMIEPGVASVETIQHAFQPGKVNLIDCDLLDAFSVQKLIGAVRPDRIFHLAAQ
                     SHVPTSWNSPSATLQDNILGQLNIFEAIRTAGIDPLIQIAGSSEEYGLVYPDEVPMKE
                     SNPLRPLSPYAVSKVTQEMLAYQYHQSYGIKSIVSRGFNHSGPRRGENFVDSSYAKQI
                     AEIEKGKREPVIHVGDLSSKRDFTDVRDMVKAYWLLLEKCRPGDVYNIGSGNTRTMQE
                     VLNMLL"
     gene            complement(1004..1426)
                     /locus_tag="VFH60_05890"
     CDS             complement(1004..1426)
                     /locus_tag="VFH60_05890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET6313348.1"
                     /translation="MADCVFCDIVEGRLPKSLVYEDGVALAFMDILPINAGHVLVIPR
                     AHFEVMSEMDEETGVHMFRVALRVQEAIRRSGVRCEGINLFLADGEAAGQEVFHVHLH
                     VFPRYTGDGFKIDYVWSAQPPREELDEVAASIRSAMNN"
     gene            complement(1419..2564)
                     /locus_tag="VFH60_05895"
     CDS             complement(1419..2564)
                     /locus_tag="VFH60_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET6313349.1"
                     /translation="MKAIILVGGQGTRLRPLTNSTPKPLLPLLNRPFLDHVLFLLKTH
                     GITDIILAVGYRSESFEPAYGDGSHLGVKLTYVKEDKPLDTGWAIKNVEQHLDAGESF
                     LVFNGDVLTDLDIADMVKFHSENGSLCTISLTPVEDVSSYGVVEVDDEGAVKRFLEKP
                     KPGTTESKWINAGTYVMETAVLDYISEPRFLDEIPAGRPFSVEYGLFPKLLEEAKPFF
                     GYRSGAYWLDMGTPERYLQAQDDLLAGRLKYALEPPGEQVQDGVWAGEGTFIDKGARL
                     TGPVVLGRGCKLRANSAVTGPASLGDGCHVGEEAQVQNVIAFEEVTFGASSNSSDSLF
                     GNGAIIGAECDIRDMTVVGDNATVGAANRLSGGRVEIGEKVPGGQNG"
     gene            complement(2874..3860)
                     /locus_tag="VFH60_05900"
     CDS             complement(2874..3860)
                     /locus_tag="VFH60_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET6313350.1"
                     /translation="MVDTSAYTAPVRALLTGAGGFVGRHLLAHLLGNTDWQLYCAARS
                     VPRNVVDSERVHWLACDLRDARQTGELVARVAPGFAFHLAAQSNVQQAFKDPEGTFVT
                     NVVGQLNLLNALRASSPHARILVACSSEQYGLARPEDIPIDEETPFRPNNPYAVSKIA
                     QDALAVQHFLSWGQQTIRVRAFNHIGPGQTDSFVASAFARQVARIESGLQEPVLYVGN
                     LEAERDFTDVRDMVTAYLLAISRGEPGEVYNIGSGKTRTIQSLLDALLASSKVPVEVR
                     QDPARMRPSDIPVLLCDASRFQQRTGWAPAILFEQTVQDILDYWREQVRTET"
     gene            complement(3975..5165)
                     /gene="metK"
                     /locus_tag="VFH60_05905"
                     /pseudo
     CDS             complement(3975..5165)
                     /gene="metK"
                     /locus_tag="VFH60_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
     gene            complement(5288..6679)
                     /gene="ahcY"
                     /locus_tag="VFH60_05910"
     CDS             complement(5288..6679)
                     /gene="ahcY"
                     /locus_tag="VFH60_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET6313351.1"
                     /translation="MVLEAKPQNTILETQGTRVKSHIKGTDPALIQKGINRIEWAEAE
                     MPVLRQIRERFEREQPLKGLRLSACLHVTAETANLMRTLKAGGADLTLCGSNPLSTQD
                     DVAAALVEEYGISTFAVKGEDTNTYYSHIVSALDHRPQITMDDGADLVSSMLFIALDR
                     LDDVHPTVAQWAGNLGADERHALLNDVVGSSEETTTGVIRLKAMEKDGVLKFPVIAVN
                     ESDTKHLFDNRYGTGQSTVDGILRATNILLPGKIMVICGYGWCGRGIALRARGMGARV
                     VVTEVQPVRALEAVMEGYEVMPLVEAAKVGDIFVTVTGDLNVIDRQHMEVMKNGAIMC
                     NSGHFNDEINIPALVDLGGGKRGKIVRDFVEEFVMADGRKLYLLADGRLVNLAAAEGH
                     PASVMDMSFANQALAAEYMVVNGPNLEPKVYVIPQELDHEIAMLKLLSLGIQIDELTE
                     EQKNYLASWESGT"
     gene            complement(6832..7221)
                     /locus_tag="VFH60_05915"
     CDS             complement(6832..7221)
                     /locus_tag="VFH60_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6313352.1"
                     /translation="MADHKDKGKGPSKHIMVVNDTEEILELFQQILREEGGYEVTLTS
                     FQPQMIEFVKEIKPDLIISDHIFGAEQMGWQFVQALKMDRDTMRIPIIVCSGAISELK
                     GNEGYLVSKNIGVLYKPFDVDELLHLV"
     gene            complement(7224..>7483)
                     /locus_tag="VFH60_05920"
     CDS             complement(7224..>7483)
                     /locus_tag="VFH60_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6313353.1"
                     /translation="ARPVEVVDPVGAGDAYLGGLVFALESGLDIQRAGRVASLAAVYA
                     VEQYGTQAHTYTREEFARRYVENFGDEAIVAEALGAVEEVK"
BASE COUNT         1307 a         2406 c         2180 g         1590 t
ORIGIN      
        1 caggagcatg ttcagcactt cctgcatggt gcgggtgttg cccgaaccaa tattgtacac
       61 gtcaccgggc ctgcacttct ctagcagcag ccagtaagct ttcaccatgt cgcgtacgtc
      121 tgtgaagtcg cgtttgctcg acaggtcccc cacatggatg accggctcgc gcttgccttt
      181 ctcgatttcg gcaatctgct tggcgtaaga ggagtccacg aagttctcgc cacggcgggg
      241 gccgctgtgg ttgaagcccc gcgagacgat gctcttgatg ccgtagctct ggtggtactg
      301 gtaggccagc atctcctgcg tgaccttgct cacggcgtaa ggcgagaggg gccgcagggg
      361 gttgctctct ttcatcggca cttcgtcggg gtagaccagg ccgtactcct ccgatgagcc
      421 tgctatctgg atgagcgggt ctatccccgc cgtccttatc gcctcaaaga tgttcaactg
      481 gcccaggatg ttgtcttgta gcgtggccga gggggagttc caagaggtgg gcacgtggct
      541 ctgcgccgcc aggtggaaga tgcggtcggg ccgcaccgcg ccaatcagct tctgcacgct
      601 gaacgcgtcg agcaggtcgc aatctataag gttgaccttg cccggttgga aggcgtgctg
      661 tatcgtctcg acgctggcga cgcccggctc tatcatgttg atctgcaagt cggtgtcctg
      721 catatgctcc aacctgctac ggcggcgata aacaccgaaa atctccacat cggcgtggtc
      781 ggcagccagg tactcggcca tatgactgcc cgcgaacccg gtgatgcccg ttaccagtac
      841 tctcaaaact tactcctcca aactgttagc cacgctatta taaggcatgg tgagacgatg
      901 tgtgggtaaa gcgcaactcg taactcgtaa ctcgtaacta ttacctgaca aaccgcattt
      961 tggctttttg ctccctttcg tgttacgtgt tacgtgttac gctttagtta ttcatcgcgc
     1021 tcctgatgct ggcggctacc tcgtccaact cttcgcgggg aggctgcgcg ctccacacgt
     1081 agtcaatttt gaaaccgtcg cctgtgtagc gcgggaatac gtgcaggtgg acgtgaaaga
     1141 cctcctggcc cgctgcctcg ccgtcagcga gaaagagatt gatgccctcg cagcgcacgc
     1201 ctgagcgacg tatcgcctcc tgcacgcgca gggcaacccg gaacatgtgc acgcccgtct
     1261 cctcgtccat ttcggacatg acctcgaagt gcgccctggg gataaccagc acatgcccgg
     1321 cgttgatagg caggatgtcc atgaaggcga gggccacccc atcctcgtag acgaggctct
     1381 ttggcagcct gccctcaacg atgtcgcaga aaacgcaatc agccattctg cccccctggc
     1441 accttctccc caatctccac acgcccaccg cttagacgat tcgcggcccc cacggtcgca
     1501 ttatcgccta ccacggtcat atccctgatg tcgcactccg ccccgattat cgcgccgttg
     1561 ccgaaaagcg agtccgacga gttactcgat gctccgaacg ttacttcctc aaaggcgatc
     1621 acgttctgga cctgtgcctc ctcgcccacg tggcagccgt cgcccaggct cgcgggcccg
     1681 gtgacggcgg agttggcgcg cagcttgcac cccctgccga gcaccaccgg cccggttagc
     1741 ctcgcgccct tgtcgatgaa ggtgccttcg cccgcccaaa cgccgtcctg cacctgctcg
     1801 ccgggcggtt cgagcgcgta cttgagcctg cccgccagga ggtcgtcctg cgcctggagg
     1861 tagcgctccg gcgtgcccat gtcgagccag taggcccccg aacggtagcc gaagaagggc
     1921 ttggcttcct ctaacagctt cgggaacaag ccgtactcga ccgagaaggg ccgtcctgcc
     1981 ggtatttcat ccaggaagcg cggctcggaa atgtagtcaa gcaccgccgt ttccatcacg
     2041 taggtgcccg cgttgatcca cttcgattcg gtggtgccgg gcttcggctt ttcgaggaag
     2101 cgttttaccg cgccctcgtc gtccacttcc acaacaccgt aagagctaac gtcctccacc
     2161 ggcgtcagcg aaatcgtgca taacgaaccg ttctcgctgt ggaacttgac catgtcggca
     2221 atatcgaggt cggtgagcac gtcgccgttg aacaccagga aggactcgcc cgcatcgagg
     2281 tgttgctcga cgttcttgat ggcccagccg gtatcgaggg gcttgtcttc cttcacgtac
     2341 gtcagcttca cgcccaggtg cgacccatcg ccgtatgcag gctcgaagct ctcggagcga
     2401 tagcccactg ccaggatgat gtcggtgatg ccatgcgtct tgagcaagaa caggacgtgg
     2461 tccaggaagg gccggttcaa cagcggcaac agcggcttgg gcgtgctatt agtgagagga
     2521 cgaagccgcg tcccctgtcc acctacaagg atgatagctt tcataggtat ctgctaaaac
     2581 gtaaaacgtg aagggtgtag ccaggggaat tctctcccgg tgagggcggc tctgctactc
     2641 ttgcaggacc aagctcccca tgtcatcctg agcgtagcga aggatcagcc tccccagttt
     2701 gactcttcct tctcaggcac taatgttcct aatgccaaca gcatccccag cgttgaacac
     2761 tctcgcctac ccacctgatc cttcgctacg ctcaggatga caaaggggga agcggaccaa
     2821 caacacggac cactgagcag gcacgaacgg tctcaccact gccacattac gccttacgtt
     2881 tcagtcctga cttgctccct ccaatagtct agtatgtcct gcacggtctg ctcgaacagt
     2941 atcgccggtg cccagccggt gcgctgctgg aagcgcgagg catcgcaaag tagcaccggg
     3001 atgtccgagg gccgcatgcg ggcggggtcc tgccgtactt cgaccggcac cttgctgctc
     3061 gccaacagcg cgtcgagcag cgactgaatc gtgcgtgtct tgccggagcc tatattgtac
     3121 acctcgcccg gctctccccg gcttatcgcc agcaggtacg ccgtgaccat gtcgcgcacg
     3181 tcggtgaagt cccgctcggc ctccaggttg cccacgtaaa gcaccggctc ctgcaacccc
     3241 gactcgatgc gcgccacctg ccgcgcgaag gccgaagcca cgaaactgtc ggtctgcccc
     3301 ggcccgatgt ggttgaaggc cctcaccctt atcgtctgct ggccccagct caggaagtgc
     3361 tgcacggcca gcgcatcctg ggcgatcttg ctcaccgcgt acgggttgtt gggccggaaa
     3421 ggggtctcct catcgatggg gatgtcctcc ggccgggcga ggccgtactg ctccgacgaa
     3481 cacgccacca gtatgcgggc atgaggcgac gacgctcgaa gtgcgttcag caggttgagc
     3541 tgccccacca cattggtgac aaaggtgcct tccgggtcct tgaaggcctg ctgcacgttg
     3601 ctctgggcgg ccaggtgaaa cgcgaagccg ggggccacgc gcgccaccag ctcgcccgtc
     3661 tgcctcgcgt cgcgcaggtc gcaggccaac cagtgcactc gctcgctatc caccacattg
     3721 cgcggcaccg agcgcgcggc acagtaaagt tgccaatcag tgttgccgag caggtgggcg
     3781 agcaggtgcc gcccaacgaa tccacccgcc ccggtcaaca gcgcgcggac cggggcggtg
     3841 taggcgctcg tatctaccac tgtttagcgc tgggtgcttg agccgacctt gcccgtcgac
     3901 tcggcgtggt ggccgttccc gtcgccggtc ctggcgtgtg cctcgtcacc gctcagagag
     3961 gccaggtcga tgcccgcctc ctcgcgcagg aggtcggcct tgtcggtctc ctcccacggc
     4021 gcggtgatgt cgtggcggcc aaagtgtccg taagcggcgg tcgacttgta gatgggctgg
     4081 cgcaggttca ggtcgcgaat gatagcgccc ggacgcaggt cgaagtgctt gtcgatgagc
     4141 gccacgatct cctcgtccgc gatgcgtccc gtgccgaagg tctccaccgc tatggagata
     4201 ggtcgggcca caccgatggc ataagagagt tgtagctcga agcgttcggc aatgcccgcg
     4261 gccacgatgt tcttcgcgac atagcgcgcc gcgtaagccg ccgagcggtc caccttcgtc
     4321 gggtctttgc ccgaaaatgc cccgccgccg tgccgggcaa tgccgccata cgtgtccacg
     4381 ataatcttgc gcccggtgag gcccgcgtca cccttggggc cgccaatcgc gaaattgccg
     4441 gtggggttga tgtagtagcg ggtattctcg tccagcaaat tgtgcgggat gaccttcaag
     4501 atcacctctc gcttgatggc ctcttcaatc tcgtcgtgcg tgatttcggg ttcgtgctga
     4561 gccgacacca ctaccgcgtc cacgcgcacg ggcttgccaa agcggtactc gaccgtcacc
     4621 tgcgccttgc cgtcggggcg gagccaccct agagtggtag tcttgcgcac ctgggcaagc
     4681 cgcttggtca gcttatgcgc cagcgcgata gtgaggggca taagctcctc ggtctcagtg
     4741 cacgcaaagc ctatcatcat gccctggtcg cccgcgccta tcgactcgat cttgtcgtcc
     4801 tcgctaagct ctccctgctt ggactccagc gcctcgtcca cgcccatagc tatatccggc
     4861 gactgctcgt ggattgacac cacgaccgcg caagtctcgg cgtcgaagcc gtacttggcc
     4921 cgcgtgtaac cggcctgctg tatcgtgtcg cgcgcgatct tctgcacgtc cacccaggcg
     4981 gtggtggtga tctcgcccat cacgacaatg aggcccgtgg tcgtggccgt ctcgcaggcc
     5041 acgcgagcgt cagggtcctg cgccaggata gcgtcgagta ccgcgtccga aatctggtcg
     5101 caaagcttgt ccgggtgccc ctccgtcacc gactccgacg taaggaacag cttcggtgac
     5161 gacataaacg tcgaagtatg cttgttatcg ttcttaccca tttcgttctc ctattttaga
     5221 ttttgggttt tagattttgg attgaggacg caaatccaaa atccaaaatc taaaacccaa
     5281 aatcgttcta tgttccgctt tcccaggagg cgaggtagtt cttctgctcc tcggtgagtt
     5341 catcgatctg gatgcccagc gacaggagct tgagcatcgc tatctcgtgg tctagctcct
     5401 gcgggatcac gtacaccttc ggctccaggt tggggccgtt gacgaccatg tactcagcgg
     5461 ccagcgcctg gttggcgaag ctcatgtcca tcacggaggc ggggtggccc tctgcggctg
     5521 ccaggttcac caaacggccg tcggccagca agtagagctt gcggccgtcg gccatgacga
     5581 actcctcaac gaagtcgcgc acgatcttgc cgcgcttgcc accgcccagg tccaccaggg
     5641 ccgggatgtt gatctcgtcg ttgaagtggc cgctgttgca catgatcgcg ccgtttttca
     5701 taacttccat gtgctggcgg tcgatcacgt tcaggtcgcc cgtgacggtc acgaagatgt
     5761 cgcccacctt cgcggcctca accaggggca tgacctcgta gccttccatc accgcttcga
     5821 gggcgcgcac cggctgcact tccgttacta ccacgcgagc acccatgcca cgggcgcgca
     5881 gggcgatacc acgcccgcac caaccgtagc cgcaaattac catgatcttg ccgggcagca
     5941 ggatgttggt ggcgcgcagg atgccgtcca ccgtgctctg gcccgtgccg tagcggttgt
     6001 cgaacaagtg cttggtgtcc gactcgttca ccgcgataac ggggaacttg agcaccccgt
     6061 ccttctccat cgccttgagc cggatcacgc cggtggtggt ctcctcgctg ctacccacca
     6121 cgtcgttgag cagcgcgtgc cgctcgtccg cgcccaggtt gcccgcccac tgcgccaccg
     6181 tggggtgcac gtcgtccagg cggtccagcg cgatgaagag catcgaggat acgaggtcgg
     6241 ccccgtcgtc catcgtgatc tgggggcggt ggtccagggc cgacacgatg tggctgtagt
     6301 aggtgttggt gtcctcgccc ttcacggcaa acgtgctgat gccgtattcc tcgacgaggg
     6361 cggcggctac gtcgtcctgg gtggagagcg ggttggagcc gcagagggtc aggtcggccc
     6421 cgcccgcctt cagggtacgc ataaggttgg cggtctcggc ggtcacgtgg aggcaggccg
     6481 agaggcggag ccccttgagc ggctgttccc tctcgaaacg ctcgcgtatc tggcgtagca
     6541 ccggcatttc ggcctcggcc cactcgatgc ggttgatgcc cttctgtatc agcgcggggt
     6601 cggtgccctt gatgtgggac tttacgcgcg tgccctgggt ctccaggatc gtattttgcg
     6661 gtttagcttc aagtaccatt cgtgttcaca ctcctagttt tggttcaaca tcacattcga
     6721 cagcgtgccg cctggttgct cttatttgct gccgttgctt tcgctcttgg tggaagtaag
     6781 atgagtctcc cgcgtgttga cgttaggatc gtcagcttcg tccagcttcc tctagaccag
     6841 gtgcagcagc tcgtccacat cgaagggctt gtacaatact ccgatgttct tggacaccag
     6901 gtatccttcg ttgcctttaa gctcgctgat tgcgccgctg caaacgatta tcggtatcct
     6961 cattgtgtcg cggtccatct tcagcgcctg gacgaactgc cagcccattt gctccgcgcc
     7021 aaatatgtgg tcgctgatga tgaggtcagg cttaatttcc ttgacgaact cgatcatctg
     7081 gggctggaac gaggtgaggg taacttcgta cccgccttcc tcgcgcaata tctgctggaa
     7141 cagctccagt atctcctcgg tgtcgttcac caccattata tgcttgctag gccccttccc
     7201 tttgtccttg tggtcggcca ttcctatttc acctcctcaa ctgcaccgag cgcctccgct
     7261 acaatcgctt catccccaaa attctcgacg tagcgccttg caaactcttc tctcgtataa
     7321 gtgtgggcct gcgtgccgta ctgctcgacc gcgtacacgg cggcaagcga ggccacccga
     7381 cccgcccgct ggatgtcgag tcccgattcg agggcgaaca ccaggccgcc caggtaggca
     7441 tcgcccgcgc ccactgggtc caccacctca accgggcggg ccg
//
LOCUS       DASRWZ010000381         3657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_923452, whole genome shotgun sequence.
ACCESSION   DASRWZ010000381 DASRWZ010000000
VERSION     DASRWZ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923452"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /gene="recA"
                     /locus_tag="VFH60_05925"
     CDS             <1..826
                     /gene="recA"
                     /locus_tag="VFH60_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET6313354.1"
                     /translation="KLGGVAAYIDAEHAFDPQYAGNLGLDLNQLLISQPDNGEQALEI
                     AETLVRSGAIDVVVVDSVAALVPRAEIEGEMGDSHVGLQARLMSQALRKLVGAISKSH
                     CCLIFTNQIRMKIGVMYGNPETTTGGNALKFYASVRMEIRRVESLKQGQDVTGNRVRV
                     KVVKNKVAPPFRTAEFDIMFNEGISRVGSLVDMGTELNVLRKSGAFYSFGDQRLGQGR
                     ENVKEFLRNNPEVADEIEAQIRSALTGGTVQPLVVGAGVAVAAGKNGASDEEEGEF"
     gene            894..1643
                     /locus_tag="VFH60_05930"
     CDS             894..1643
                     /locus_tag="VFH60_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HET6313355.1"
                     /translation="MSDVITALQADPMDANKVHVFIDGKHALAVSLDVAASERLTVGQ
                     LCPPQRLEELLQAQELDDIYQKAIAFLSYRPRSAREVEQRLRKKGFTPEQISAAMDRL
                     RAQHYIDDHEFARFWVGNRMTFNPRGPRLLRSELRQKGVPSEIVEEVLQQQVETQEEL
                     VQQAEELKVGDNPGEDEPVPGTDLANALALARKRLRTYGNLEPQAARRRLSGFLARRG
                     YGFDVIDAVWRRVSTQDDEDEDEGEHELFDD"
     regulatory      1749..1843
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2009..3199
                     /gene="gcvT"
                     /locus_tag="VFH60_05935"
     CDS             2009..3199
                     /gene="gcvT"
                     /locus_tag="VFH60_05935"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET6313356.1"
                     /translation="MEERSISADVMAKKEDEDRRAQPQDVADPARTPLYQEHVDLGAR
                     MVEFGGWMMPVQYKRGIIEEHKAVRSTVGIFDTCHMGEFVLQGPDAQLLANTYTSNNV
                     AALGTTQAQYSFICNEEGGVQDDTIVYQFPDHNFIVVNAAPLKADFQWLQELAGRINA
                     DVQITNLSDEMGKLDVQGPEAAALVQRFTDTDLSTIKYFWATFGDIDGVPCRISRTGY
                     TGEDGFELFFPVEETVRLWRLFVENGAAPSGLGCRDTLRLEASLPLSGSDVAAGYNRN
                     PVWAGFGRFVKLDKGVDFIGREALQRAASDPGGERLINFFITERGVPRAHYPIMKDGE
                     QVGEVTSGSYGPTLDKYLGMGWVRAGLHEVGTRLEVLIRERPVAIEVLPRPMYKRPKV
                     KRNT"
     gene            3286..>3657
                     /gene="gcvH"
                     /locus_tag="VFH60_05940"
     CDS             3286..>3657
                     /gene="gcvH"
                     /locus_tag="VFH60_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET6313357.1"
                     /translation="MDNPRNYRYTKEHEWASLEGEVATMGITNYAATQLGDMVYVDMP
                     TVGATVAQGDSMGALESVKAAADFYSPLGGEVIERNERLLDEPGLLNSDPYGEGWFVK
                     VRTSDASEFEALMPADEYDEFE"
BASE COUNT          769 a         1119 c         1177 g          592 t
ORIGIN      
        1 gaagctgggc ggcgtagcag cctacatcga tgccgagcat gccttcgacc cccagtacgc
       61 gggcaatctg ggcctcgacc tgaaccaact gctgatttcg cagcccgaca acggcgagca
      121 ggccctagaa atcgccgaga cgctggtgcg ctcaggcgcg attgacgtgg tagtggtcga
      181 ctcggtggcg gcactggtgc ccagggctga aatcgaaggc gagatgggcg acagccacgt
      241 aggcttgcag gcccgcctca tgtcgcaggc cctgcgcaag ctggtgggcg cgatcagcaa
      301 gagccactgc tgcctcatct tcaccaacca gatacgcatg aagatcggcg tcatgtacgg
      361 caacccggag acgaccaccg gcggcaacgc actgaagttc tacgcctccg tgcgcatgga
      421 gatccgccgc gtcgagtcgc tcaagcaggg gcaggacgtc accggcaacc gcgtgcgcgt
      481 aaaggtggtc aagaacaagg tcgcgccccc gttccgcacc gccgagttcg acatcatgtt
      541 caacgagggc atcagccgcg tcggttccct ggttgacatg ggcaccgagc taaacgtgct
      601 gcgcaagagc ggcgcgttct actcctttgg cgaccagcgc ctcgggcagg gtcgcgagaa
      661 cgtgaaagag ttcctgcgca acaaccccga agtggccgac gagatagagg ctcaaatcag
      721 gagcgctctg accggcggca ctgtacagcc gctcgtagtg ggtgcaggtg tggctgtggc
      781 tgccggtaag aacggcgcat cggacgaaga ggaaggcgag ttctaacaga cgcgcttgat
      841 ggaagggggc gagcaccacg ctcgcccctt tctgcttaac gcgggaggtg ctcatgtcgg
      901 acgtaataac ggcgctacag gccgacccga tggacgcgaa caaggtccac gtcttcatag
      961 acggcaagca cgcgctggcg gtctcgctgg atgtggcggc aagcgagcgg cttaccgtgg
     1021 gtcagctttg cccgccccag aggttggaag agcttctcca ggcgcaggag ctggacgaca
     1081 tataccagaa ggcgattgct ttcctatcgt accggccccg gtcggcgcgg gaggtagagc
     1141 agcgacttcg caagaagggc ttcacgccgg agcagattag cgcggctatg gacaggctgc
     1201 gcgcccagca ctacatcgac gaccatgagt tcgcccgctt ctgggtgggc aaccgcatga
     1261 ccttcaaccc gcgcggccct cgcctgttga ggagcgaact gcggcaaaag ggcgtgcctt
     1321 ccgagatcgt ggaagaggtg ctccaacagc aggtcgaaac gcaagaggag ctggtgcaac
     1381 aagccgagga gctgaaggtg ggggacaacc ccggcgagga cgagcctgtg cccggcaccg
     1441 accttgcgaa cgccctggcc ctggcacgca agcggctgcg cacctacggc aacctggagc
     1501 cgcaggccgc acgtcgccgc ctctccggct ttctcgccag gcgcggctac ggcttcgacg
     1561 tcatcgacgc cgtctggagg cgcgtctcga cccaggacga cgaagatgag gacgagggcg
     1621 agcacgaact gttcgatgac tagaaccaga ctcgatagag ggattgttga acatcagcac
     1681 gcaagtccgc accctgacgg caggtaatgg ctcgttgact gagggcatgg gccgtgctat
     1741 aatggccgga gcgggttgcg ggagagactc tgcacagtcg cggagcgccg aaggggaaag
     1801 gccagggccg aaactctcag gcaaattgga ccgtaatctc gaatacctct ggagagttcc
     1861 ctcgcaggga cgccgacgga gcaaggccga tgcgccaatc tcgcaggtag aaggacagag
     1921 gaggcaagtt gcttcctact gtcttttttg ttgcgtcaaa atggctaccg gccccatctc
     1981 ccgactatct ttaccgcatg gagagtatat ggaagagcga tccatcagcg ccgatgtaat
     2041 ggccaagaag gaagacgagg accggcgcgc ccagccgcag gacgtggccg accccgcccg
     2101 cactcccctc taccaggagc acgttgacct cggcgctcgc atggtggagt tcggcggctg
     2161 gatgatgccg gtgcagtaca agcggggcat tatcgaggag cacaaggccg tgcgctccac
     2221 ggtgggtatc ttcgacacct gccacatggg cgagttcgtc ctccaggggc cggacgcgca
     2281 gctcctggcg aacacctaca cctccaacaa cgtggccgcg ctcggcacca cccaggccca
     2341 gtactccttc atctgcaacg aagaaggcgg cgtgcaggac gacaccatcg tctaccagtt
     2401 ccccgaccac aatttcatcg tggtcaacgc cgcccccctc aaggccgatt tccagtggct
     2461 gcaagagctt gccgggcgca tcaatgccga cgtgcagata accaacctct cagacgagat
     2521 gggcaagcta gacgtgcagg gtccagaggc cgcggccctg gtgcagcggt tcacggatac
     2581 ggacctctcc actatcaagt acttctgggc cactttcggc gatatagacg gcgtgccctg
     2641 ccgcatctct cgcacgggct acacgggcga ggacggcttc gagcttttct tccccgtcga
     2701 ggagacggtg cggctctggc ggctgttcgt ggagaacgga gcagcaccca gcggcctcgg
     2761 ctgccgcgat accttgcgcc tggaggcgag cctgcccctg tccggcagcg acgtagccgc
     2821 cggctacaac cgaaaccccg tgtgggcggg ctttggccgc ttcgtcaagc tggacaaggg
     2881 cgtcgatttc atcgggaggg aggccctgca gcgggcggct agcgaccccg gcggcgagcg
     2941 gttgataaac ttcttcatca ccgaacgcgg cgtccctcgc gctcactacc cgatcatgaa
     3001 ggacggcgaa caggtaggcg aagtcaccag cggctcatat ggcccgacgc tcgacaagta
     3061 cctcggtatg ggctgggtcc gggcgggcct gcacgaagtc ggcacccgcc tggaagtgtt
     3121 gatacgcgaa cgcccggtgg caatagaagt gctgccgcga ccgatgtaca agagacctaa
     3181 agtaaaacgt aacacgtaac acgtaattcg taattcacaa gttacgtgtt acgtgttacg
     3241 tgttacgcga caagaccgaa agcaccaaat ttcaaggagg ccaggatgga caacccgcgc
     3301 aactacaggt acaccaagga gcatgagtgg gcgagcctcg aaggcgaagt cgctacgatg
     3361 ggcattacca actatgccgc cacgcaattg ggcgacatgg tgtacgtgga catgcccacg
     3421 gttggggcga ccgtcgcgca gggcgactcg atgggcgcgc ttgagagcgt aaaggccgcc
     3481 gccgacttct acagccccct gggcggcgag gtcatcgagc gcaacgagcg tctgctggac
     3541 gagccgggcc tgctcaactc cgacccgtat ggcgagggtt ggttcgtcaa ggtccgcacc
     3601 tccgatgcct ccgaatttga ggccctcatg ccggccgacg aatacgacga attcgag
//
LOCUS       DASRWZ010000382         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1197578, whole genome shotgun sequence.
ACCESSION   DASRWZ010000382 DASRWZ010000000
VERSION     DASRWZ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197578"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFH60_05945"
     CDS             <1..237
                     /locus_tag="VFH60_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313358.1"
                     /translation="DANSVVDGWVVPGTGSGWRYAFAIPYVGYALSTLLSDIGRLLLL
                     VIPTVLFAGLMLVEIWLPARKPEQAPDAAMPAGA"
     gene            241..>1531
                     /locus_tag="VFH60_05950"
     CDS             241..>1531
                     /locus_tag="VFH60_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313359.1"
                     /translation="MTRGRIAGAVALCALCVLLSVAPSIKVTFALLTARTANPNARFA
                     TTSLYAPGSLVATPSGRSSVLSWTAGQNGNGYKVLGLGNGTSSACPASTSASYTAITT
                     TTGLSYTDANRSPASVPQGTWYCYQVQTTYQSWSSVLNNPVAGVQVGVVATSAQLVNL
                     SASKLDSGDQFIFTFNQAIDTTTGPVSGNSVCSNGTTIWIGSTAISGGCAATETVNVG
                     KITGGTLIENTRWAATYAWSNSNTRLTVTLGSRTAGNKAPVLSASTWTFTPTTTSTKL
                     LSAAGAFHICDSNTGGGNCTPTLSIGTSGAVAGAQQSAAQSAPADADSAPATATPAKA
                     ITATATLTPTATSSPAATRTATSTTQEAVASATPSSSPVPSHTPAPPTASATSSPTST
                     KTPTSTATATRTSTSTATASATASPSRTPTASATATAT"
BASE COUNT          303 a          567 c          419 g          242 t
ORIGIN      
        1 gatgccaaca gcgtggtcga tggctgggta gtgcccggca caggcagcgg ctggcgctac
       61 gccttcgcca tcccctatgt aggctacgca ttatccaccc tcctgagcga cattggccgc
      121 ctgctcctcc tcgtcatccc aaccgtattg ttcgccggtc tgatgctcgt ggagatatgg
      181 ctgcccgcca ggaagcccga acaggcccct gacgccgcca tgcctgccgg agcttaggcc
      241 gtgacacgcg ggcggattgc cggtgccgtt gccctgtgcg cgctctgcgt gctacttagc
      301 gtcgccccca gcatcaaggt tacctttgcg ctgctcacgg cacgcacggc caaccccaac
      361 gcccgctttg ctacgacctc tctgtacgcg cctggtagcc tggtagccac tccgagcggg
      421 cgaagtagtg tgcttagttg gacggcgggg cagaacggca acggctacaa ggtgctgggg
      481 cttggcaacg gcacgagtag cgcctgcccc gcgagcacca gcgccagcta cacggccatc
      541 accactacca ccgggctcag ctacaccgac gccaatcgct cccccgcaag cgtgccacag
      601 ggcacatggt actgctacca ggtgcagacg acttaccagt cgtggagcag cgtcctgaac
      661 aaccccgtcg cgggagtcca ggtaggtgtg gtcgctacca gcgcgcaact ggtcaacctc
      721 agcgccagca aactggatag cggggaccag ttcattttca ccttcaacca ggcgattgac
      781 accactacgg gtccagtcag tggtaatagc gtgtgtagta acggcacgac gatatggatt
      841 ggcagtactg ccatcagcgg cggctgtgcc gctaccgaga ccgtgaacgt gggtaagatc
      901 acgggtggta cgttaatcga gaatacccgc tgggctgcta cctacgcttg gagcaacagc
      961 aatacgaggc ttaccgtaac tctcggctcg cgcaccgccg gcaacaaggc cccggtgctg
     1021 agcgcatcca cctggacgtt taccccgacc acgaccagca ccaagctgct ctcagccgcc
     1081 ggggcctttc acatctgtga tagcaacacg ggtggcggga actgcacccc cacacttagc
     1141 ataggcacgt ccggtgccgt agcgggtgcc cagcagagcg ccgcgcaaag cgcacccgcg
     1201 gacgccgaca gcgccccagc cacagctacc ccggcaaagg ccatcaccgc caccgctact
     1261 ctcaccccca cggcgaccag cagccctgca gcaactcgca ccgccaccag cacgacccag
     1321 gaggccgtag ccagtgccac cccttcctca tcgcctgtac cgtctcacac accggccccg
     1381 cccaccgcct ctgccacttc ctcccctacc tctacaaaga cacctacaag cacagccacc
     1441 gccacgagga cttcgacaag cacggccacc gcctcggcta cggccagccc ctctcgcact
     1501 cccacagcct cggctaccgc cacggcaaca g
//
LOCUS       DASRWZ010000383         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1197645, whole genome shotgun sequence.
ACCESSION   DASRWZ010000383 DASRWZ010000000
VERSION     DASRWZ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197645"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="VFH60_05955"
     CDS             complement(<1..1724)
                     /locus_tag="VFH60_05955"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET6313360.1"
                     /translation="MAATTHATQAQAQTGPALKVGLAALAVVAGLALGAAAGFGVPQT
                     YLVAGLAAPFLLLLTLARPHWAVAIYAVLVYADLLSVLVQHHGMPPLARFAGAMLLSA
                     VLGYRIIIRREGLVPDRVTWWLAAYGGMVALGLVYARHTDSVMVNVIEFGRIFLTYLI
                     IVNAITTPARLRILLWGLLGAGVFLAMLTIYQTLTGQFDNDFGGLATYRVSDIAAGSD
                     APRPGGTIGDANYYGQLLLVVLPIGLYLMFAGKSLAARIGGLAASTSLVIAVVFTYSR
                     GDALALGAIVVAAAIYKRPHPIFLVGAVVALMAALPLMPPGYVARLTTVLDVVQGNKQ
                     TIYTEDSIRGRAGATQAAIDMFLDHPLLGVGRENYASYQLEYISGTSLAKVAKGIPPH
                     NLYLEVLAEHGLLGVLVVGGLLVAVWRALREARRRYLSQGNYQQAELAGWLGIGLFGY
                     LVSGMFLHGAFLYILGLQIALIVALRQMSRAADVAPTIPILPVQSDLASDAIYAPLPP
                     DEDIDQRRTGKLAGVSRWAHALQSRITTGKLTPPDETGTPDEAPAEQPVTEQRPPARD
                     LINIWLRAA"
     gene            complement(1729..2469)
                     /locus_tag="VFH60_05960"
     CDS             complement(1729..2469)
                     /locus_tag="VFH60_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313361.1"
                     /translation="MEVRTYLGIILRYWWVVLLLTLVAGGSAAALVSIRTPVYTTHAR
                     VVARPSITLTETREIVDMLGQMGTRQLSGTFAQAFTSALVKTEAREEAGLSEADAADY
                     PLEANVLPDTTVIEISGKGPDPQVLAKYLNATVQATVSSTKELFRVMDLVPLEAAAVP
                     EEPTSPQPMRDIPLGAGLGFGLGIMLALAIDYLRGGSRTPEMQLRALPPHPTQLERLA
                     VNERRTEFLRRQQENALPPGQQAETYRE"
BASE COUNT          477 a          841 c          797 g          487 t
ORIGIN      
        1 gccgccctca accagatgtt gatgaggtcc cgcgccggag gtcgttgctc cgtcaccggt
       61 tgttcggcag gtgcctcgtc cggcgtgccc gtttcgtctg gtggtgttag cttacccgtc
      121 gtaattctac tttgcaaggc gtgcgcccac cgagacaccc ccgcgagctt ccctgtgcgc
      181 cgttggtcta tgtcctcatc aggcggtaaa ggggcgtaga ttgcgtccga ggccaggtcg
      241 ctttgcactg gcaggatagg tatggtggga gccacgtcag ccgcccgcga catctgccgg
      301 agtgcgacga tgagcgcgat ctgcaagcca aggatgtaga ggaacgcccc atgcaggaac
      361 ataccgctca ccaggtagcc gaacagccct atcccaagcc agccagccaa ctcggcctgc
      421 tggtaattcc cctggctcag gtagcggcgg cgcgcctccc gcaacgccct ccatacggct
      481 accagcaggc ccccaacgac caacaccccc agcagcccat gttccgccag tacttccagg
      541 tagagattgt gcggcgggat gcctttggcg accttcgcca ggctcgtgcc cgatatgtat
      601 tcaagctggt atgatgcgta attttccctc ccgacgccaa gcagtggatg atccaggaac
      661 atatctatcg cggcctgagt agctcccgcc cggcctcgga tcgagtcttc ggtgtatata
      721 gtctgcttgt taccctgaac cacgtcgagc acggtagtga gcctcgccac gtagccgggc
      781 ggcatgaggg gcagcgccgc catgagggcc acgaccgcgc ccaccaggaa aatggggtgc
      841 gggcgcttgt agatggctgc cgctaccact atcgccccca gcgccagggc gtcaccacgc
      901 gaataggtga atactacagc gataactagg ctcgtgctcg ccgctagccc cccgatgcgt
      961 gcggccaggc tcttccccgc gaacatcagg tacagcccta tgggaaggac caccagcagg
     1021 agttggccgt agtagttggc gtcgccgata gtgccgcctg ggcggggcgc gtctgagcct
     1081 gccgcgatgt cgctaacccg gtacgttgcc agtccgccga agtcattatc gaactggccg
     1141 gtgagcgtct ggtatattgt cagcatagcc aggaatacgc ccgctcccag caacccccac
     1201 agcaagattc gcagccgggc aggtgtggtg atggcgttga ctatgattag ataggtcagg
     1261 aagatgcgcc cgaactcgat gacgttcacc atgacgctgt cggtgtgccg ggcatagacc
     1321 agcccaagcg ccaccatccc accgtaagcc gccagccacc acgtaaccct gtcgggcacc
     1381 agcccctcgc ggcggatgat tatgcggtag cccagcaccg cgctcaggag catcgccccg
     1441 gcaaagcgtg ccaggggagg catgccatga tgctgcacca gcacgctcag gaggtcggcg
     1501 taaacgagca cggcgtagat agccacagcc cagtgaggtc tagccagcgt gaggagcagg
     1561 aggaagggag cggccagccc ggcgacgagg taggtctggg gcacgccgaa gcccgcagcc
     1621 gcgcccaatg ccagccccgc gaccaccgcc aatgccgcaa gccccacctt caaggcgggg
     1681 ccggtctgcg cttgggcctg agttgcgtgg gtggtcgcgg ccatgctgct actcgcggta
     1741 cgtctcggcc tgttgccccg gcggcagggc gttttcttgc tggcggcgca aaaactcggt
     1801 ccgccgctcg ttgaccgcca acctttctaa ctgggtgggg tgcggcggca atgccctcag
     1861 ttgcatctca ggcgttctcg acccgccgcg caggtagtct atagccagcg cgagcatgat
     1921 gcccagccca aagcccagcc ccgcgccgag cggtatatcc cgcataggtt gtggcgacgt
     1981 aggttcttca ggcacggcgg cggcctcaag gggcaccagg tccatcacgc ggaaaagctc
     2041 tttcgtgcta ctaacggtgg cctgcaccgt cgcgttcaga tacttcgcca gcacctgtgg
     2101 gtccggcccc ttgccgctga tctctatcac ggtcgtgtcg ggcaggacgt tggcttcaag
     2161 cgggtagtct gccgcgtccg cctccgacag tcccgcctct tcccgcgcct ccgtcttcac
     2221 cagggccgat gtgaacgcct gcgcgaaggt gccgctcagt tggcgcgtgc ccatctgccc
     2281 cagcatatcc actatctctc gcgtctcggt gagggtaatt gaggggcgtg ccactacccg
     2341 cgcatgcgtg gtgtagacgg gcgtcctgat gctcaccagg gcagccgcac tcccgcctgc
     2401 cacgagcgtg aggagtaaca ccacccacca gtagcgaagg attatgccta gatatgtgcg
     2461 aacttccatg tttggtcctc tgcgtgaaac gtaaaacgta ttgcgtagtt cgtattgcgt
     2521 aaaacgtgat gcgtgaagtg tgattatttg gattaggaac tgcttgtggg taggccgacc
     2581 ctgattccat gtcatcctga gc
//
LOCUS       DASRWZ010000384         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_717711, whole genome shotgun sequence.
ACCESSION   DASRWZ010000384 DASRWZ010000000
VERSION     DASRWZ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717711"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFH60_05965"
     CDS             <1..633
                     /locus_tag="VFH60_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HET6313362.1"
                     /translation="VPPVKALLLPPLTEASFQIAASGRQARVIVALPDQIDTQESVVE
                     VKTEDGWALADPDRDILKLAVVERHAGTGNVGLGFVQGFHIKSGALASSVAHDSHNVV
                     VVGANDRDMLLAANAVIEMGGGLSAARGGEVLARLALPVAGLMSDQPLGPVRDALLVL
                     LHHARELGCPLSNPYMAMAFLALPVIPALKLTDKGLVDVHKFSLVPLFVS"
     gene            665..2515
                     /gene="mutL"
                     /locus_tag="VFH60_05970"
     CDS             665..2515
                     /gene="mutL"
                     /locus_tag="VFH60_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET6313363.1"
                     /translation="MSRLSADLHNPAVIHVLPEDVASKIAAGEVVERPASVVRELVDN
                     AIDAGARHVRIEIRAGGRELIRVTDDGCGVRPDDVPRAFLRHATSKIKTADDLWAVKT
                     LGFRGEALFSIAAVSRMSFLSRPQDAAVGYEMVVEGGHVTSHGAKGAPLGSVVTVRDL
                     FYNLPARLKFLKSPQAESAHITALVQQYALAHPMLRFTLTSEGRQTFQSPGDGDLRTA
                     ACCVYGTDVARAFLPVGIEPEEPDDQWDSLLDLMVYGYVSPPAHSRSNRQAMLFFVNR
                     RAIQSRMLQYAVAEAYHSLLMVGRHPICIVHLSIAPSLLDVNVHPAKAEVKFRDERVV
                     FSAVQKAVRSALQAHVPAPAFGSRGNEGWSLGNGATGIEAEFSTSAGYAPGLSQGREH
                     TQADLWDESGNATASRAGDEGIEPLPPPKLRSLPPLRVVGQVGTTYIIAEGPDGLFLV
                     DQHAAHERVLYEQLGAAMVGGSLMVQPLLTPVPLELTAHQRSMVEPVLPLLAELGFEL
                     EAFGDSALLVRAVPAIYASSNRDMGRALLELIDEVMSGTAPDRWREEMAITLACHSAI
                     RAGQVLSLDEMRALLGQLELCHYPRSCAHGRPTMLHLSQMQLEREFGRRA"
     gene            complement(2520..>3343)
                     /locus_tag="VFH60_05975"
     CDS             complement(2520..>3343)
                     /locus_tag="VFH60_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET6313364.1"
                     /translation="GGAMQSFRYVPGRSVLHSLNPSIKLLTITLVMVAITFIFDVATP
                     LAFFVLTVAAMLTLGRLSPSRVLRSLVPFLLIALGFVVLNTIFHRPLATETLLFSLGP
                     LMISWEGLEIGLSIGLRVLFLVSTSLLFVATTDPQELVLSLVQQARLGYKLAFALLVA
                     YRFVPLFAEEYANIRAAATVRGIGRDRGLFARVKSLRRDAVPLLASAIRRSERLAIAM
                     DSRAFGAFPTRTYRKTMKVTTRDWLFFFGGLGLSAALLAVLVATGLTRGIGVLFQ"
BASE COUNT          620 a         1055 c         1116 g          552 t
ORIGIN      
        1 gtgccgcccg tgaaggctct gctgctgcca ccgctgacgg aagcctcgtt ccaaatcgcg
       61 gcgtcaggaa ggcaggccag ggtcatagtg gccctgcccg accagataga cacgcaggag
      121 agcgttgtcg aggtcaaaac cgaggatggt tgggcgctgg ccgacccgga ccgtgatata
      181 ctcaagttgg cggtggtaga gaggcacgcc gggaccggca acgtcggcct cggcttcgta
      241 cagggcttcc acatcaaatc tggggctttg gcttcgagcg tggcccacga ttcgcataac
      301 gtggtggtgg tcggggccaa cgaccgggat atgctgctgg ccgccaacgc ggtgatagag
      361 atgggtggtg gcctcagcgc ggcgcgcggt ggtgaggtgc tcgcccggct ggccttgccc
      421 gtggcggggc tgatgtcgga ccagccgctc ggccccgtgc gggacgctct gctggtgttg
      481 ctgcatcacg cgcgagagct tggctgccct ttgtcgaacc cttacatggc tatggcgttc
      541 ctggccctgc cggtgatacc cgccctcaag ctgacggaca aggggctggt ggacgtacac
      601 aagttctcgc tggtgccgct gttcgtctcc tgagaccgct cagtcacaag aaagtagtct
      661 ccccatgtcc aggctttccg ccgacctgca caatccagcc gtgatacacg tcttgcctga
      721 ggacgtagcc tccaaaatag cggcgggcga agtagtcgag cggcccgcgt cggtcgtacg
      781 cgagcttgtt gacaacgcca tagacgccgg ggcacgccac gtacgcatag agatacgagc
      841 cggtgggcgg gagctaataa gggtgaccga tgacggctgc ggcgtgcgtc ctgacgacgt
      901 gccccgcgcc ttcctcaggc acgccaccag caagatcaaa acggcggatg acctctgggc
      961 ggtgaagacg ctcggcttca ggggggaggc gctcttctca atcgccgcgg tgagccgtat
     1021 gagcttcctc agcaggccgc aggacgcggc ggtcggctac gagatggtgg tggaaggcgg
     1081 gcatgttacg tcccacggtg caaagggcgc gcccctcggc agcgtcgtga cggtccgcga
     1141 cctcttctac aacctgcccg cccgcctgaa gttcctcaag agtccgcagg ccgaatcggc
     1201 gcacataacc gcgctggtgc aacagtacgc cctcgcccac cctatgctgc ggttcacact
     1261 caccagcgag gggcggcaga cgttccagtc ccccggcgac ggtgacctgc gaacggcggc
     1321 ctgctgcgtc tacgggacgg acgtcgcacg ggcgttcctg ccggtgggca tcgagccgga
     1381 agagccagac gaccagtggg acagcctgct ggacttgatg gtgtacggct acgtgtcgcc
     1441 cccggcgcac tcgcgctcca accggcaggc gatgctcttc ttcgtcaaca ggcgcgccat
     1501 ccagagccgc atgttgcagt acgcggttgc cgaggcgtac cactcgctgt tgatggtggg
     1561 ccggcaccct atctgcatcg tgcacctcag tatcgcgcca agcctgctcg acgtgaacgt
     1621 gcaccccgca aaggccgaag tgaagttccg ggacgagcgg gtggtgttca gcgcggtgca
     1681 aaaggcggtc cgctccgcgt tgcaggccca cgtacctgcc cccgccttcg ggtcgcgcgg
     1741 caacgaaggc tggagcctgg gcaacggtgc taccggtatc gaggcggagt tttcgacctc
     1801 ggcaggttac gcgccaggtc taagccaggg gcgcgagcac acgcaggcag acctctggga
     1861 cgagtcaggt aatgcgaccg cctcacgcgc cggagacgag ggcatcgagc cgctgccccc
     1921 gcccaagctc cgcagcctgc cacccttacg cgtggtgggc caggtgggga cgacctacat
     1981 cattgccgaa ggccccgacg gcctatttct cgtggaccag cacgcggcgc acgaacgggt
     2041 gctgtacgag cagttggggg cggcgatggt gggcgggtcg ctcatggtgc agccgctgct
     2101 cacgccggtg ccgctggagc ttaccgccca ccagcggtcg atggtcgagc ctgtgctgcc
     2161 gctgctggcg gaattgggat tcgagcttga agcgttcggt gacagcgcgc tgctggtaag
     2221 ggcggtaccg gcgatttatg cctcttccaa ccgcgatatg ggccgcgctc tgctcgagct
     2281 gatagacgag gtcatgtcgg gcaccgcacc ggaccgctgg cgggaggaaa tggcgattac
     2341 cctggcctgc cacagcgcta tcagggccgg gcaggtgctg tcactggacg agatgcgggc
     2401 gctgctgggc cagcttgagc tttgccacta cccccgctct tgcgcccacg gcaggccgac
     2461 tatgctccac ctatcgcaga tgcagcttga gcgcgagttc ggacgccgcg cctaggccgc
     2521 tactggaaca gcacccctat cccgcgcgta agtccggtag ctacaagcac ggcaagcaag
     2581 gccgccgaca accccagccc cccgaaaaag aagagccaat cgcgtgtcgt gaccttcatt
     2641 gtcttgcggt aggtgcgagt ggggaaagcc ccgaacgccc ggctgtccat cgcgatagcc
     2701 agccgctccg accgccggat ggcgctcgcc agcagtggca cggcgtcacg gcgtagcgat
     2761 ttgacgcggg caaagagtcc ccggtcgcgg ccaattcccc tcaccgtcgc cgcggctcta
     2821 atgttagcat actcctcggc aaacaggggc acaaagcggt aggctacaag cagagcgaac
     2881 gccagcttgt agccgagcct ggcctgctgg acgaggctca acacaagctc ctgcgggtcc
     2941 gtggtagcga cgaacagcag cgaggtggac acaaggaaga gcacgcgcag ccctatcgag
     3001 aggccgatct caagcccttc ccagcttatc atgagcggcc ccaggctgaa tagaagcgtt
     3061 tcggtcgcga gcggcctatg gaagatggtg ttcagcacca caaaaccgag cgcaatgagc
     3121 aggaatggga caaggctcct gagcacccgg ctgggcgaga ggcgtcccaa tgtcagcatc
     3181 gcggccacgg tgagcacgaa gaaagcaagg ggagtcgcca cgtcgaatat gaaggtgata
     3241 gcaaccatga cgagtgttat cgtcagcagc ttgatggagg ggttcagcga gtggaggacg
     3301 cttcgacccg gcacgtaccg gaacgactgc atagctcctc cac
//
LOCUS       DASRWZ010000385         1977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_101258, whole genome shotgun sequence.
ACCESSION   DASRWZ010000385 DASRWZ010000000
VERSION     DASRWZ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101258"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1789)
                     /gene="recJ"
                     /locus_tag="VFH60_05980"
     CDS             complement(59..1789)
                     /gene="recJ"
                     /locus_tag="VFH60_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET6313365.1"
                     /translation="MEPRSPHWHIHPRLSDEQVNRVRRELSHLALTPLMVQLLANRGI
                     VDLEEIDTFIQPQDVSQHPDPSMLTGMQPAVERICQALNTHEKVVVYGDYDADGVTST
                     ALLTTALRHFGADVEPYVPNRMNEGYGLNHNAVRELGAKGTRLLITVDCGISGREEVE
                     VARQAGMDVIVTDHHHLPSDLPNAAACINPKQFVAGCECYQELAGVGVAYQLVRALVK
                     RCGKPKRLRNKDLLGLVAIGTVADVVQLKGANRPLVQQGLAAIPYNSLPGLRTMLEYA
                     GLKPGSIDTDRVGFIIGPRLNAAGRVDDARTAYELLMTEDAYYARQLAQKLEAQNRRR
                     QAMMNSIVEQARERVKRMDDGTRIIVLHDSGWPAGVVGLVAGRLVEEFGRPAVVIEQG
                     EEESRGSARSVPQFNMVEALGEASDLLVKYGGHSAAAGFTVKTTNIEELSRRLCATCD
                     QQLQGEHLRSTISADAELPVNMVDDDTLEQINRMSPFGAGNPSPLFMARGVRVVSAST
                     VGDNDAHLKLIVADRNNGHAPLEAIAFRYGYMAENARRKGQVDLVYSIERREWKGSTH
                     LQLRIKDIKV"
BASE COUNT          324 a          620 c          641 g          392 t
ORIGIN      
        1 gagccgcttt ttacgtgtta cgcgttacgg ttacgcgtta cgtgttacgc gttacgcctc
       61 acaccttgat gtccttgatc cttagctgga ggtgggtgct gcccttccat tcgcggcgtt
      121 cgatggagta gacgaggtca acctggccct tgcgtcgggc gttctcggcc atgtagccgt
      181 agcggaaggc aatcgcttcc aggggggcgt ggccgttgtt gcggtcggcc acgatgagct
      241 tgaggtgggc gtcgttgtct cccacggtgg aggcgctgac cacccgcacc cccctggcca
      301 taaagagcgg cgaagggttg cccgccccga acggcgacat ccggttgatc tgctccagcg
      361 tgtcgtcgtc caccatattg acgggtagct cggcgtcggc gctaatcgtg gagcgcaggt
      421 gctcgccctg tagctgctgg tcgcacgtgg cgcacaggcg gcggctcagt tcctcgatgt
      481 tggtcgtctt taccgtgaaa ccggcggcgg cgctatgtcc cccgtacttc accagcaggt
      541 cgctagcttc acccagggct tccaccatgt tgaactgcgg gacgctgcgt gccgagccgc
      601 gactctcctc ttcgccctgc tcaatcacca ccgccgggcg gccaaactcc tccaccagcc
      661 tgcccgccac cagccctacc accccggcgg gccacccgga gtcatgcagc acgataatgc
      721 gggtgccgtc gtccatgcgc ttcacgcgct ctcgggcctg ctccactatc gagttcatca
      781 tggcctggcg gcggcggttc tgggcctcga gcttctgggc aagctgccgg gcgtagtacg
      841 cgtcctctgt catcagcagc tcgtaggccg tgcgggcgtc gtccacgcgt cctgccgcgt
      901 tgaggcgggg gccgatgata aacccaaccc ggtcggtgtc gatggagccc ggcttcagcc
      961 ccgcgtattc gagcatggtg cgcaggccgg gcaggctgtt gtaggggata gcggccagcc
     1021 cctgctgcac cagcgggcgg ttggcaccct taagttgcac cacgtcggcc acggtgccaa
     1081 tcgccaccag cccgagcagg tccttgttgc gcaggcgctt gggcttgccg caacgtttca
     1141 cgagggcgcg caccaactgg taagccacgc ccacgcccgc aagctcctgg tagcactcgc
     1201 aaccggccac gaactgcttg gggttgatgc acgcggcggc gttcggcagg tcggagggca
     1261 gatggtgatg atcggtcacg atcacgtcca tcccggcctg ccgcgccacc tccacctctt
     1321 cgcgccccga aatgccacag tccaccgtga taagcaagcg ggtgcccttc gcgccaagct
     1381 cgcgtaccgc gttatggttg aggccgtagc cctcgttcat gcggttgggc acgtaaggct
     1441 ccacgtcggc cccgaaatgg cgcagggcgg tggtgagcag ggctgtggag gtcacgccgt
     1501 ccgcgtcgta gtcgccatac acgacgacct tctcgtgcgt gttgagggcc tggcagatgc
     1561 gctccacagc gggctgcatg ccggtgagca tggaggggtc ggggtgctgg gaaacgtcct
     1621 gcggctggat gaaggtatct atctcctcaa ggtccacaat gcccctgttc gccaggagtt
     1681 gcaccatgag gggggtgaga gcgagatggg aaagctcccg acgtacgcgg ttgacctgct
     1741 cgtcagacaa acgagggtgg atgtgccagt gtggggagcg tggttccaag gccggatact
     1801 ctcttttaca caatacttaa gctgctcaat acgactcact tgctctcgcc aaatcatagc
     1861 acaccgggat gtgtaaaggc aacaagcagg ttaggcaggg tctggtcggg tgcgccgcct
     1921 tagccctgtg gctaatgaag attatgaact tgataaggta gtggcctgta ggggcag
//
LOCUS       DASRWZ010000386         3353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_237887, whole genome shotgun sequence.
ACCESSION   DASRWZ010000386 DASRWZ010000000
VERSION     DASRWZ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237887"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1574)
                     /locus_tag="VFH60_05985"
     CDS             complement(60..1574)
                     /locus_tag="VFH60_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6313366.1"
                     /translation="METIKAPREMESRNAPIDMSAEEFRRLGHALVDRLASFMEGLPG
                     MPVTPGESPSQVRSALDAVRPLPKQGTDPEALLTNATSLLLDHSLFNGHPRFFGFITA
                     GPAPIGMLGDLLASAVNPNVGGYELSPIATEIEAQTVRWIAEMLNYPTNCGGLLVSGG
                     NMANFVGFLAARRARATWDVRAEGMDAHHGKMRVYTSAETHTWVQKAADLFGLGTEAV
                     RWIPTDSQQRMNTQALRRQIQQDRDNGDLPIMVIGTAGSVSTGAIDPLPEIAAICREH
                     NLWFHVDGAYGGFAAALPDVHADLRGLSEADSVAVDPHKWLYAPLEAGCALVRSREAL
                     HGTFEFHPAYYHMIDMAEDPAINYHEYGMQNSRGFRALKVWLALQHVGLEGYRRMIAD
                     DIALSKEMFRAAGEHPELEACTQALSIATFRYVPPGVQPGTEDAEAYLNRLNTELLDR
                     LQAGGETFMSNAIIDGKFLLRACIVNFRTSIADVLAVPEIVARIGRQVHEEMGR"
     gene            complement(1880..3262)
                     /locus_tag="VFH60_05990"
     CDS             complement(1880..3262)
                     /locus_tag="VFH60_05990"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6313367.1"
                     /translation="MTTHEDKATIPAEHVDFPNANGSAESKRAGRQANSRRTRRPRVV
                     IVGAGFGGLNAARALGDKGVVVLVLDCNNYHGFWPLLYQVATAGIESEAIAYPVRAIL
                     RKHRNINFRMNTVTRVDLNRKVVYTEGRPVAYDYLILAAGSANNYFGNNALARHTYGL
                     KDIDEAEHLRNRLLYCFEQAAAETDPQKRRELMTFVVVGGGPTGAELSGAFSELINFV
                     LKKDYPHLDVTQARVILVEATDKVLSAFPEDLQRSAMKKLMSMGIELRMNSKVQSVES
                     GLVTFEDGSTLQAGAVVWAAGVKGSELGNTLGVEQGKGGRVKVEPALHLPGHPDVFAV
                     GDMAYLEGYKGDQAYPMVAQVAIQQGKQAARNILEHVAGREMQPFHYFDQGSMATIGR
                     RYAILDAFGVRLSGRLAWFGWLFIHIIYLIGFRNRLIVLTNWTYNYFSYERAVRLITG
                     KDLEMQPSVE"
BASE COUNT          539 a         1029 c         1114 g          671 t
ORIGIN      
        1 ttagctgtcc tttgcgctct ttgcgccttt gcggtttctc ctcctactca gtccatcgcc
       61 taccgcccca tctcctcgtg cacctgccgc cctatccgcg ctacgatctc aggcacggcc
      121 agcacgtctg caatggaggt gcggaagttg actatgcagg cgcgcagcag gaacttgccg
      181 tcaatgatgg cgttggacat gaacgtttca cccccggctt ggaggcggtc gagcaactcg
      241 gtgttgagcc tgttgaggta ggcttcggcg tcttcggtgc cgggctgcac gccggggggc
      301 acgtagcgga aggtggcaat gcttagggcc tgggtgcacg cctccaattc ggggtgctct
      361 ccggcggcgc ggaacatctc tttggagagg gcgatgtcgt cggctatcat gcgcctgtag
      421 ccttccaggc cgacgtgttg cagggccagc cacaccttga gggcgcggaa gccccgcgag
      481 ttttgcatgc cgtactcgtg gtagttgatc gcggggtcct cggccatgtc gatcatgtgg
      541 tagtaggccg ggtggaactc gaaggtgccg tgcaacgcct cccggctgcg gaccaacgcg
      601 caacccgcct ccaacggcgc gtagagccac ttgtgcgggt caaccgccac cgagtcggcc
      661 tcggacagcc cccgcaggtc ggcgtgcacg tcgggcaggg cggcggcaaa gcccccatac
      721 gccccgtcca cgtggaacca gaggttgtgc tcgcggcaga tggccgctat ctccggcagg
      781 gggtcaatcg cgccggtgct caccgagcct gccgtaccga ttaccatgat gggcaggtcg
      841 ccgttgtctc ggtcttgctg tatctgcctg cgcagggcct gggtgttcat gcgctgctgg
      901 ctgtcggtgg gtatccaacg caccgcttcc gtgccgaggc cgaacaggtc ggcggctttc
      961 tgcacccagg tgtgggtctc ggccgacgtg tagacgcgca tcttgccgtg gtgggcatcc
     1021 atgccctcgg cgcgcacgtc ccaggtagcc ctggcgcggc gggcggcgag gaagccgacg
     1081 aagttcgcca tgttgccgcc gctcaccagc aggccgccgc agttggtggg atagttcagc
     1141 atctcggcta tccagcgcac cgtctgcgcc tcgatttcgg tggcgatggg cgagagctcg
     1201 tagccgccga cgttggggtt gaccgccgag gcgagcaggt cgcccagcat gccgatgggg
     1261 gctggccccg ccgtgatgaa gccgaagaag cgcgggtgcc cgttgaaaag cgagtggtcg
     1321 agcaggaggc tggtggcatt ggtcagcaag gcttccgggt cggtgccctg cttcggcaac
     1381 ggcctgacgg cgtccagcgc gctcctgacc tggctggggc tttcgccggg ggtcaccggc
     1441 ataccgggca ggccctccat gaaactcgcc agccggtcca cgagcgcgtg accgagcctg
     1501 cgaaactcct ccgcgctcat gtcaatggga gcgttgcgcg actccatctc gcgcggggcc
     1561 tttatcgtct ccatacagta cacttctcct tctgtagaaa cgggggagcg ggtgattagt
     1621 aattacaaat tacaaattac gaatgaggga tgtcatcgat gcgcataaga tgctacatgc
     1681 gtatgcgaag cttgcgagga caacgagggg agaaggcgga cgcccggtgc ttgccctttg
     1741 agggaggcgt tggtcctagt atagcataag ggccgggaac gaggggcaat gtaggtggct
     1801 tgcatggacg cccggcgata gccctgtggg gcgtcccccg caggacgccc aggcgcggcg
     1861 cgtaattcgt aattcgtcat tactctacac tgggctgcat ctccaggtct ttgccggtga
     1921 tgaggcggac ggcgcgctcg taggagaagt agttgtaggt ccagttggtg agcacgatta
     1981 ggcggttgcg gaagcctatc aggtagatga tgtggatgaa gagccagccg aaccaggcaa
     2041 ggcgaccgct caggcgcacg ccgaacgcgt cgagtatggc gtagcggcgg ccaatcgtcg
     2101 ccatactgcc ctggtcgaag tagtggaagg gctgcatctc gcggcccgcg acgtgttcca
     2161 ggatgttgcg cgccgcctgc ttgccctgct ggatcgctac ctgggccacc atcgggtagg
     2221 cctggtcgcc cttgtagcct tccaggtagg ccatatcgcc caccgcaaac acatcggggt
     2281 ggccgggcag gtgcagcgca ggctcgacct tcacgcgccc gcctttgccc tgctccacgc
     2341 ccaacgtgtt gccaagctcc gagcctttga cgcccgccgc ccacaccacc gcgcccgcct
     2401 gtagcgtgct gccgtcctcg aacgtcacca ggccgctctc caccgactgc actttgctgt
     2461 tcatccgcag ctcgatgccc atgctcatta gcttcttcat cgcgctccgc tggaggtctt
     2521 ctgggaaggc cgacagcacc ttgtcagtgg cctccaccag tatgacgcgg gcctgcgtaa
     2581 cgtcgaggtg gggatagtcc ttcttgagca cgaagttgat cagctcggag aaagcgccgg
     2641 aaagctccgc gccggtgggg ccgccgccta cgaccacgaa ggtcattagc tcgcggcgct
     2701 tctgggggtc ggtctcggcg gccgcttgct cgaagcagta gagcaggcgg ttgcgcaggt
     2761 gctcggcctc gtctatgtcc ttgaggccgt aggtgtggcg ggccagggcg ttgttgccga
     2821 agtagttgtt ggcgctgccc gccgccagta tgaggtagtc gtaggccacg gggcggccct
     2881 cggtgtagac cactttcctg ttgaggtcca cgcgcgtgac ggtgttcatg cggaagttga
     2941 tgttgcggtg cttgcgcagg atggcccgga ccgggtaggc aatcgcctcc gactcgatgc
     3001 ccgccgtcgc cacctggtag agcaggggcc agaagccgtg atagttgttg cagtcgagca
     3061 ccagcacgac tacgcctttg tcgcccaggg cacgtgccgc gttcagtccg ccgaagcccg
     3121 cgcccacgat caccacgcgg ggcctgcggg tacggcggct gttggcctgc cgcccggctc
     3181 tcttgctttc ggccgagccg ttcgcgttgg ggaagtcaac gtgttcggcg ggaatagtgg
     3241 ctttgtcttc atgcgttgtc atggtatgca cctcgccgtt ctcatatggg actttccttc
     3301 tactcttatt atatgtgaat aaaatcacaa aatcaatccg ccgtagggtg tat
//
LOCUS       DASRWZ010000387         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_375986, whole genome shotgun sequence.
ACCESSION   DASRWZ010000387 DASRWZ010000000
VERSION     DASRWZ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375986"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VFH60_05995"
     CDS             complement(<1..1014)
                     /locus_tag="VFH60_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6313368.1"
                     /translation="MIQSASFGAWLKERRKALGMTRDDLADQVGCAAVTIIKIERGQR
                     RPSRQVAECIAAALRISPDEREAVVQWARGTLDAAVPSVPALSTQASTNAPAEPRGLP
                     EAVPARLDNLPAALNSFVGREQEVGRVGNLLLRADVRMLTLTGPPGIGKTRLSLTVAS
                     ALRGEFEHGVWFVSLAPIQDPSLVLPTIAQVVGLKEASAQTIESVLHGYLHDKRLLLV
                     LDNLEQLLEAAPLLSELLAAAPNVKALVTSRSPLHVYGEHEFPVPPLDLPDIDTLPAP
                     EVLAGYPAVALFAERAQSVKPDFQLTSENAAAVARLCVRLDGLPLAIELAAARSKLFT
                     PASM"
     gene            1302..1769
                     /locus_tag="VFH60_06000"
     CDS             1302..1769
                     /locus_tag="VFH60_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HET6313369.1"
                     /translation="MLVEDDRSRASADAKRGTMPVADDEQAIRDIARRLEEGWNSGNG
                     QAFAAPFAEDSDYVVVDGRHIKGRPANAEGHQFIFDTIYRGSTNSMEVEGVRFIRPDV
                     AIAHVRHHLKFSRDGQPAEGHARSTWVLTKDQGQWAIAAFQNTPIQPPADGNA"
     gene            complement(2081..>3108)
                     /locus_tag="VFH60_06005"
     CDS             complement(2081..>3108)
                     /locus_tag="VFH60_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6313370.1"
                     /translation="RPHMTKKKREERTVRLGIIGAGLAVKLLHWPPLSRMPERFSVVA
                     VADIDANAALEVAGLASGCRSTTDYHELLASEDVEAVLVSLPIHLNAQVLAEAAIAGK
                     HVLCEKPVGGNLQQAREVVSAVRDLPVVVAIAEQFHHRRDIRQAQAWIEEGRIGKPFL
                     VDAWNRYWTDTRKGFGSTPWRQDSQYRGGAITDAAVHQAALLRHLGGEVEQLHAFTKL
                     VHPELSGVDTMVLNLRYRSGVLGRFMFTAAAAEAGSPYMQATVYGTEGTIVLEDGKLR
                     LCGKDNARESQEYDTGEAFYDQLVNYHRAITVGEAVVSTPEEALRDLEILMRAYDSAE
                     SRSVVLL"
BASE COUNT          561 a         1009 c          977 g          561 t
ORIGIN      
        1 catcgacgcc ggggtgaata gcttgcttcg ggcagcagcc agttctatcg ccaacggcag
       61 cccgtcaagg cgcacgcata gccgcgccac agcggcggca ttctcggaag tcagttgaaa
      121 atcgggcttc acggactgcg cgcgctccgc gaagagcgca acggcagggt acccggccag
      181 cacttcgggc gcaggcaggg tatcgatatc gggcaggtcg agcggcggta ctggaaactc
      241 gtgctcgcca tacacgtgga gcggcgagcg gctcgtaacc agggctttca cattgggagc
      301 cgcggccagt agctccgaca ggagcggcgc ggcttccagt aactgctcta aattgtcgag
      361 caccagcagg aggcgcttgt catgcaggta accgtgcagc acgctctcta tcgtctgggc
      421 cgacgcctct ttcaacccta ctacctgggc gatggtggga agcaccaggc tggggtcctg
      481 gatgggggca agcgatacga accacacccc gtgctcgaac tcacctcgca gcgcggaggc
      541 cacggtgaga ctgaggcgcg tcttgccgat gccgggaggc ccggtgagcg tcagcatgcg
      601 cacgtcagcc ctgagcagca ggttgcctac gcgccctacc tcctgttcgc ggcccacgaa
      661 cgaattgagc gcggcgggca ggttgtcgag gcgagcgggg actgcctctg ggaggcctcg
      721 cggttcggcc ggcgcgttgg tagaagcttg agtggagagg gcaggtacag aggggaccgc
      781 cgcatcgagc gtgcccctgg cccactgcac gaccgcctcg cgctcgtcgg gagagatgcg
      841 cagggctgcc gctatgcact cggctacctg ccgggacggc cgccgctgcc cgcgctcgat
      901 cttgatgatg gtgacggcgg cgcagccgac ctggtcggcc aggtcatccc tggtcatgcc
      961 cagcgccttg cgacgctcct ttagccacgc cccaaacgat gcactctgta tcatgcgcag
     1021 tccagtcctg gatacgccga gtacagggcg ctctcatggc agccccctgg ccggtgtacc
     1081 cctgtttgta tccccactgt acccctgctg gtaccaccca ttatagcacc agttttgcta
     1141 tgctccgcac atgacacatc agggtgttgc agcggccacg aggggctgcg aactaatggg
     1201 acttcgccca ccaccgagcc ggccagtcgc acaaccagat ggcgatgtcg ctgccgggtg
     1261 agtagcaacc cgcgtagcca tcaagcaaag gagtaaggac catgttagtg gaggacgacc
     1321 gctccagggc gtctgcggat gccaaacgag gcactatgcc tgtagcggac gatgagcagg
     1381 cgatccgtga catcgcgcgg aggttggaag aggggtggaa cagcggcaat gggcaagctt
     1441 tcgccgcccc tttcgccgag gactcggact acgtggtcgt ggatggcagg catatcaaag
     1501 ggcgacccgc caacgccgag gggcaccaat tcattttcga cacgatctac aggggcagca
     1561 ccaacagcat ggaggtggag ggcgtgcgct tcatccgccc tgacgtagcc atcgctcacg
     1621 tgcggcatca cctcaagttc agccgggacg gtcagcccgc cgagggacac gccaggagca
     1681 cctgggtcct gaccaaggac cagggccagt gggcaatcgc tgcctttcaa aacaccccga
     1741 ttcaaccacc ggcagacggt aacgcttagc gttctgcctc tcgtcctggt ccttgtctca
     1801 cctgtgaacg gctatcgctt cgagtggcaa ggacctggcc cgacctttcg ttacggtaaa
     1861 tccctttgag taaaggagcg gcagcatgtc aacgataagc ctgagtcaat ccatgatcaa
     1921 tcggaggctt acctgggtga taacacacgt gatgtttgcc ggtgtgcctc tcacagcgcg
     1981 tgcctcacgg cccgccagag ggtcgggcac aatagcctag atgatctggc accttcgccc
     2041 ctggtaggct gtggactacg cacaacacaa aggtgcgatg ctacagcagc accacgctcc
     2101 tgctctcagc cgagtcgtag gcccgcatga gtatctccag gtcgcgtagc gcctcttccg
     2161 gcgtgctcac gaccgcctcg cccaccgtga tggcgcggtg gtaattgacc agttgatcgt
     2221 agaaggcctc acctgtgtcg tactcctgcg attcgcgggc gttatccttg ccacacaagc
     2281 gtagtttgcc gtcctccaac acgatcgtgc cctcggtgcc gtacacggta gcctgcatgt
     2341 aggggctgcc cgcttccgcc gccgctgccg tgaacatgaa cctccccagg acgccgctgc
     2401 ggtagcgcag gttcaacacc atcgtatcga ccccgcttag ttccgggtgg accagcttgg
     2461 tgaaggcgtg cagttgctct acctccccgc ccaggtgccg caggagggcc gcttggtgca
     2521 cggcggcgtc ggtaatcgcc ccgccccggt actggctgtc ctgccgccag ggggtcgagc
     2581 cgaagccctt tcgggtgtcg gtccagtagc ggttccaggc gtccaccagg aagggcttgc
     2641 cgatacggcc ctcctctatc cacgcctgtg cctgcctgat atctcggcgg tggtggaact
     2701 gctcggctat cgcgaccacc acgggcaggt cgcgcaccgc gctcactacc tctctcgcct
     2761 gctgcaagtt gccacccacc ggcttctcgc acagcacgtg cttaccggca atcgcggcct
     2821 ccgccagcac ttgagcgttg agatggatgg gcagagagac cagcacggcc tccacgtcct
     2881 cagaggcgag cagctcgtgg tagtccgtgg tcgaacggca cccgctcgcc agcccggcca
     2941 cctctagcgc ggcgttcgcg tcgatgtcgg ccacggcgac gacgctgaag cgctcaggca
     3001 tgcggctgag tggcggccag tggagcaatt tgacggcaag cccggccccg atgataccga
     3061 ggcggaccgt tcgctcttcg cgctttttct ttgtcatgtg tggtctgt
//
LOCUS       DASRWZ010000388         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_992094, whole genome shotgun sequence.
ACCESSION   DASRWZ010000388 DASRWZ010000000
VERSION     DASRWZ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992094"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            445..2526
                     /locus_tag="VFH60_06010"
     CDS             445..2526
                     /locus_tag="VFH60_06010"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET6313371.1"
                     /translation="MSIDSIPTPPNAANDTERQQADTLDGSDPGGAQPVPTPDPAASE
                     GTTAEDQSSNGPSTLPLSVVDAPSGTSTTPLPGLGGPPTPLLEMGTLLGGRYEVVLFM
                     TSLGNTNIYRVLDQQGFRRCWACGSTNSAPGDMYCVECGAQLTGRYYRVQEFSASPGE
                     TPDDPTQTTEQLDPNSPINPQPALERSEGSEIPNPKSDSSLTIPVPEAILANRVGGVV
                     QAVDAIAAPELGRVYVVWEEAYGRTLSSWLPDAGTDIFSQIQAGQSGQLSIDEPSDEQ
                     ALAWMAQSAEILANLHAEGILGCNVELNNLLVQLGDRVLLLDPSECRQADGVDTSASQ
                     ASDVRALASELERWYMSVRRDTGALSIAEEQAGAAAGNGRLVVASEVTGPLANMLNPS
                     VALSKAREGSLPTAAAFAKVLREIYEASRPLRNLQLWSGRASDVGRVRQINEDSVLTF
                     EATVLEHEGGLPVGLYVVADGMGGHQSGEVASSIAVRTIVAVVNSSLIGPMLAGDPVA
                     CDPITCTNLLRQAIMEANRRISDLARERHSDLGTTAVAAIMVGNQLCVANVGDSRAYF
                     WHEGQATPITRDHSLVAQLVAAGQIKPDDIYTHPRRNEIYRALGESRLAETDVDVFTR
                     RLQPGDGLLVCSDGLWDFVRDPDISAAINAGAEPQAICHALIDLANQGGGEDNISAIF
                     VRVVPSKRSKE"
BASE COUNT          470 a          897 c          768 g          465 t
ORIGIN      
        1 tcatcctgag cgtagcgaag gaacagtagc ccgtgtttga ctctcccttt tgttgcacaa
       61 gagttcctct agccgccatc atccccaagg ttgaagactc tcgcctaccc acctgatcct
      121 tcgctacgct caggatgaca aggggggagt gtagccctga atgacgaaac ataggtcaaa
      181 tcgcgccttc ctctccttcc tctattgaag tccctaatct cagcgcgttc tctgcgtcct
      241 ctgcgtctct gcggtgaaaa acaatcctaa tcttggcgcg ctcttggcgt ccttggcgtc
      301 ttggcggttc cgtctcttcc taatttagtc ctatctcatc gtttgcccgc cccaaccctt
      361 tatgctatat ttgcactgtc ttccattgcc gcgcgtattc cgctcgcccg catgcgcgaa
      421 accccctata aggtctcatc gatcatgtct atagactcca tcccgacgcc gcccaacgcg
      481 gccaacgaca cagaacgtca gcaggccgat accctcgacg gctccgaccc aggcggcgct
      541 caacccgttc caacacccga cccggcggct tccgaaggca ccaccgccga agatcagtcc
      601 tcgaacgggc ctagcacttt gccgctctcc gtcgtggacg cgccctccgg caccagcacg
      661 acccccctgc ccggcctcgg cggtcctccc accccgctgc tcgaaatggg cacgctgctg
      721 ggcgggcgct acgaggtggt gctcttcatg accagcctgg gcaacaccaa catctaccgc
      781 gtgctcgacc aacagggctt ccgccgctgc tgggcctgcg gctccaccaa ctcagcccca
      841 ggcgacatgt actgcgtgga gtgcggcgcg caactcaccg gacgctacta ccgcgtccag
      901 gagttcagcg cctccccggg cgaaaccccc gacgacccca cccagaccac cgaacaacta
      961 gacccgaact ccccaataaa tccgcaacct gcccttgagc gaagcgaagg gtccgaaatt
     1021 ccaaatccca aatccgattc ctccctcacc atccccgtgc ccgaagcgat acttgccaac
     1081 cgggtgggag gcgtggtgca ggcggttgac gccatcgcgg cccctgagct agggcgggtg
     1141 tacgtggtct gggaggaggc ttacgggcgc accctgtcgt cgtggctgcc cgacgcgggt
     1201 accgacatct tctcgcagat acaggcgggc cagtcgggcc agctttctat tgacgaacct
     1261 agcgacgagc aagccctcgc ctggatggcc caatcggccg aaatcctcgc caacctgcac
     1321 gccgagggca tcctcggctg caacgtcgag ttgaacaacc tgctggtgca gttgggcgac
     1381 cgcgtgctcc tcctcgaccc ctcagagtgc cgccaggccg atggcgtgga tacctcagcc
     1441 tcccaggcct ccgacgtgcg cgccctggcg agcgagcttg agcgctggta catgtcggtc
     1501 cgcagggaca ccggggcgct atcgattgcc gaggagcagg ccggtgccgc ggcgggcaac
     1561 ggcaggctcg tggtggcaag cgaggtcacc ggcccgctcg ccaacatgct caacccgtcg
     1621 gtggccctct ccaaggcccg cgaaggttct ttgcccacgg ctgccgcctt cgccaaagtg
     1681 ttgcgcgaaa tctacgaggc cagcaggccc ttacgcaacc tgcaactctg gagcggccgt
     1741 gcctcagatg tgggccgcgt gcgccagata aacgaggaca gcgtgctcac gttcgaggcc
     1801 acggtgctcg agcacgaagg tgggctgccg gtgggcctgt acgtggtggc cgacggcatg
     1861 ggcgggcacc agagcggtga ggtggcttcg tccatcgcgg tgcgcacgat tgtggcggtg
     1921 gtcaattcgt cgctgatagg ccctatgctg gcgggcgacc cggtggcctg cgaccccatc
     1981 acgtgcacca acctgctccg gcaggccata atggaggcca accgccgcat ctccgacctg
     2041 gcccgcgagc gccattccga cctgggcacc acggcggtgg cggctatcat ggtgggcaac
     2101 cagttgtgcg tagccaacgt gggggatagc cgcgcctact tctggcacga gggccaggcg
     2161 acccccatca cgagggacca ctcgctcgtg gcgcagcttg tagcggccgg gcagatcaag
     2221 ccggacgata tttacaccca cccgcggcgc aacgagattt accgcgccct gggcgagtcg
     2281 cgcctggccg agaccgacgt ggacgtgttc actcgccgcc tccaaccggg cgacggcctg
     2341 ctggtctgct ccgacggcct ctgggacttc gtgcgcgacc ccgacatctc ggccgccatc
     2401 aacgcgggtg ccgagccgca agccatttgc cacgccctca tcgaccttgc taaccagggt
     2461 gggggcgagg acaacatcag cgcaatcttc gtccgtgtcg tcccctcgaa acgtagcaag
     2521 gagtagttgc gtagagggag agtaacgagt gatgagtgat gagtgatgag gattgagtga
     2581 ggagtcttgc caatgctggg
//
LOCUS       DASRWZ010000389         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1060772, whole genome shotgun sequence.
ACCESSION   DASRWZ010000389 DASRWZ010000000
VERSION     DASRWZ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060772"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VFH60_06015"
     CDS             <1..1133
                     /locus_tag="VFH60_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6313372.1"
                     /translation="KESHQLFLEPEGWRTAEVYVQGCSTSLPEDVQWRLIRSIPALRE
                     AELTRIGYAVEYDAVPPSEITPWLESKRVAGLFLAGQLNGTSGYEEAAGQGIMSGINA
                     ALYCRRLQGASTYYVPPVHAPESAGEQLRVAIEEGLEAVREARPLVLPRHLAYIGVML
                     DDLTGMEHREPYRLMTSRAEYRLLLRSDNADLRLSALGRALGLVSRERYDELLRKDEI
                     IAGTLRKASQGVITAPVAARLRAHGLEPPEQGRHTTMLEYLRRPTTEYRALGALLPEL
                     DLSDPIIDEAAQQAEIAAKYSGYVVKQESEVARTRKLEGRAIPLDFPYMEIGGLKKEA
                     QEKLSRFRPATVGQASRIAGVTPSDIHVLLVHLQRLGEKAPT"
     gene            complement(1130..>2121)
                     /locus_tag="VFH60_06020"
     CDS             complement(1130..>2121)
                     /locus_tag="VFH60_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313373.1"
                     /translation="LYLLLAEVERHRRGADKRPWLRRGLGVVALLVGFSPMLFLVYRF
                     VFGSPYYWGNPTTWKDFFSLVTGGPFHNQVMAFGSDPGAQLGRIGFGIGELFVQYTAV
                     GIVLGLAGLVALWRVRRAEAGLLALMLLGNFLFAMNYSLVGYLYFIPTYLIWGVFMSF
                     GAGWLLHTLFLSGRAWQNEAVGRAVPAVLGVLLLCVVSLLAARRYESIDLSWQTAVRD
                     QTLTMLKAAEPNASLYLDWEELSVVRFYRLVYGMRTDLALHSGDPADWPKNVYCDVTA
                     GTPAYVGKFAGAIPAGVASDFELEPAAIGWKVTAILNPDRYEVPPCGMCATCR"
BASE COUNT          412 a          725 c          667 g          317 t
ORIGIN      
        1 acaaggagag ccaccagctc ttcctcgagc cggagggctg gcgcaccgcc gaggtgtacg
       61 tgcaagggtg cagcacgtcg ctgccggagg acgtgcagtg gcggctcatt cgctcgatac
      121 ccgccttgcg cgaggccgag cttacacgca tcggctacgc ggtggagtac gacgcggtgc
      181 ctcccagcga aatcaccccc tggctggaga gcaaacgtgt ggcggggcta ttcctggccg
      241 ggcagctaaa cggcacgtcg ggctacgagg aggcggcagg acagggcatc atgtcgggca
      301 tcaacgccgc gctttactgc cgccgcttgc agggtgcttc cacctactat gtgccccctg
      361 tgcatgcgcc ggagagcgcg ggcgagcagc tacgggtcgc gatagaggag gggctggaag
      421 ccgtacggga ggcgaggccg ctcgtgctgc cccgccacct ggcctatatc ggcgtgatgc
      481 tggacgacct caccggcatg gagcaccgcg aaccgtaccg cctgatgacc tcccgcgccg
      541 agtaccgcct gctcctgcgc tccgacaacg ccgacctgcg tctctccgcg ctgggccgcg
      601 ccctgggcct agtcagccga gaacgctacg acgaactgct gcgcaaagac gagatcatag
      661 ccggcaccct gcgcaaggcg agccagggag tcattaccgc gcctgttgcc gcacgcctgc
      721 gagcgcatgg tctcgaaccg cctgagcagg gacgccatac gaccatgctt gaatatctgc
      781 gccgccccac caccgagtac agggcacttg gcgcgctcct ccccgaacta gacctatccg
      841 accccatcat cgacgaggcg gcgcagcagg cggagatagc cgccaagtac tcgggctacg
      901 tggtgaagca ggagtcggaa gtggcccgca cgcgcaagtt ggagggacgc gccattcccc
      961 tcgacttccc gtacatggag ataggcggcc tgaagaaaga ggcccaggag aagctatcgc
     1021 gcttccgccc cgccaccgtt gggcaggcct cccgtatcgc gggcgtcaca ccctccgaca
     1081 tccacgtgct gctggtgcac ctgcaaaggt tgggcgagaa ggcccctact tagcggcagg
     1141 tggcgcacat gccacacggt ggcacttcgt agcggtccgg gttcagaatc gcggtcacct
     1201 tccaaccaat cgcggcgggt tctagctcga agtcgcttgc cacgcccgcc ggtatggccc
     1261 ccgcgaattt ccccacgtac gcgggggtgc ccgccgtcac gtcgcagtac acgttcttgg
     1321 gccagtcggc agggtcgccg gagtgcaggg caaggtcggt acgcatgccg tacacgaggc
     1381 ggtagaagcg aactaccgaa agctcctccc aatcgaggta caggctggcg ttgggttcgg
     1441 cggccttcag catggtgagc gtctggtcgc gcacggccgt ctgccagctc aggtcgatgc
     1501 tttcatagcg acgcgccgcc agcagcgaca ccacgcacag cagcagtacc cccagcacgg
     1561 ccgggaccgc gcgtcccact gcctcgtttt gccatgccct gcccgagagg aacagcgtgt
     1621 ggagcagcca gcccgcgccg aacgacatga acacacccca aatcaggtac gttgggatga
     1681 agtagagata acctaccagc gagtagttca tggcgaacag gaagttgccc agcaacatga
     1741 gcgccagcag cccggcctcg gcccgcctga cccgccacaa cgctacaagc cccgccagcc
     1801 ctagcacaat acccactgcg gtgtactgca cgaaaagttc gcctatccca aagcctatgc
     1861 ggcccagttg cgcccctggg tcggagccga aagccattac ctggttgtgg aacggccctc
     1921 ccgtgaccag gctgaagaag tccttccagg tggtcgggtt accccagtaa tagggggagc
     1981 cgaacacgaa gcggtacacc aggaagagca tggggctgaa ccccaccagc agcgccacga
     2041 cccccagccc gcgccgcaac cagggccgct tatctgcgcc tcgccggtga cgctctactt
     2101 ccgccagcag caggtagagg c
//
LOCUS       DASRWZ010000390         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1060790, whole genome shotgun sequence.
ACCESSION   DASRWZ010000390 DASRWZ010000000
VERSION     DASRWZ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060790"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..1121)
                     /locus_tag="VFH60_06025"
     CDS             complement(303..1121)
                     /locus_tag="VFH60_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HET6313374.1"
                     /translation="MTTEQEQERRASTPESQHGRDLRLSVVVPVYNETLRMGQHLPEL
                     LSYLHAQDYPYEIVVVDDGSTDGTPELAEGLLAGEARARVLAQPRNRGKGHALKVGML
                     AAEGRYVLFTDADLSTPPAELDKFWSWLEEGYDVVIGSRKMPGANIVKHQSRWRESLG
                     KVFTWLTNHIATKDISDVTCGFKCFTQDAARTLFGLSVIDDWSFDAEILFLAQRFRYR
                     IKEVPVTWHDESGTKVRLWKDAVKSLRGLLRIRMNASRGLYKGGAKRQRAATAR"
     gene            complement(1197..2429)
                     /locus_tag="VFH60_06030"
     CDS             complement(1197..2429)
                     /locus_tag="VFH60_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HET6313375.1"
                     /translation="MMCGPGKADGRGGQQKRKKIAILGWYGSANAGDEAVLQSVVESL
                     RRAGHDDLLVLSTNPNATASTYHVQSVPRNPLDPTPLRAVLQSQALVLGGGGLIQDSS
                     SVYNLPLYALYVALARLRGIPVFGWGLGVGPLYTKLGRLLARFVVRSSRYFSVRDTGS
                     ARALRAAGVRAEQVRVTADPAFLVEPTPVPLEGDAGHKPTVIFSVRHRLHDSPGLNPR
                     YLLPVGVRHRLKLETRPGLDEDRRFARSVAHGMRCCVEEFGARVVLLPFWAERDDEVL
                     RQVEQEALRLGVPGEAIAWAGVRHTPSDLAGYIAGADMLVSMRLHALIFGALAGVPAL
                     ALSYVPKMRALMSLLGARRWVVEVQTRVPSQEEIEMKLRQLWALRAAEGEKLAAAAAR
                     LRARAEQDARDIVTLLDA"
     gene            complement(2426..>3203)
                     /locus_tag="VFH60_06035"
     CDS             complement(2426..>3203)
                     /locus_tag="VFH60_06035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313376.1"
                     /translation="EVVPTGVNMLEFADQFTPQAMRNLQMAAGSRLRNRKVLLTVGRL
                     IERKGVVHFLTEVMPRIIAQRQDICYLIVGEGPQRELIETRIASLHLEDYVVLVGRVD
                     DDTLRAAYHLCDLFVMPNIPVQHDIEGFGLVALEAAAAGRYVVASRLDGIPEAIVPGQ
                     NGILLDPLDSESFADNILALLADDEQREALGRKARKYVQGHYSWDIVARRYQRLFMRV
                     IHDGHGHNQGQSQGQPSQAGARSGKATETEPHEAPGARQE"
BASE COUNT          535 a         1083 c          986 g          599 t
ORIGIN      
        1 caggtgagta tccttcgctg atacacagga gtcatgccga gccgtcagtg cgacgatagg
       61 cgctgtagag ttagagacgg aaccgccaag acgccaagaa cgccaagagc gcgccaagtt
      121 aaggtaggtg ttccaagtaa aaagggccag ggtacgtgtt gtcgtaccct ggcccttttt
      181 gcaccacctg aacctggcct aatctctgcg taacctttgc gtcctctgcg tctctgcggt
      241 gaaccgttct tattcttggc gtgtccttgg cgtccttggc gacttggcgg ttccgtctcg
      301 ccctatcgtg cggtggcggc ccgctggcgt ttagcgcctc ccttatacag gccgcgcgag
      361 gcgttcattc ggatgcggag caagccgcgc agggacttta ccgcgtcttt ccagaggcgc
      421 actttggtgc ccgattcgtc gtgccaggtg acgggcacct cttttatgcg gtagcggaag
      481 cgttgtgcca ggaagaggat ttcggcgtca aagctccaat cgtcaatcac cgagaggcca
      541 aatagcgtgc gggcagcgtc ctgggtgaag cacttgaagc cgcaagttac gtcagaaatg
      601 tcttttgtgg ctatgtggtt cgttaaccaa gtaaacacct tacccagcga ctcgcgccag
      661 cgggactggt gcttgacgat gttggctccc ggcatcttgc gcgagcctat caccacgtcg
      721 tagccttcct ccaaccagct ccagaacttg tccagttccg cgggtggcgt cgacaggtcg
      781 gcatcggtga agagcacgta ccgtccttcc gccgccagca tccccacctt gagcgcgtgc
      841 cccttcccac ggttccgggg ctgcgccagc accctggcgc gcgcttctcc ggccagcaac
      901 ccttcggcca gttcgggcgt gccgtccgtc gagccatcgt ccacgaccac tatctcgtag
      961 gggtagtcct gcgcgtgcag gtaggacagt aactccggta ggtgctggcc catgcggagc
     1021 gtttcgttat agaccggcac cacaaccgat agcctgaggt ctcgcccgtg ctggctctcc
     1081 ggtgtggaag cgcgtctctc ttgttcctgt tcggttgtca agtgcattct ctcacgtaat
     1141 gtaggtcggg gcggttggaa cgccttgcag aacatagcac acgccctctc gcggtgtcaa
     1201 gcgtccagga gcgtcactat atcgcgggcg tcctgctcgg cacgcgctct cagcctggcc
     1261 gccgcagccg caagcttttc gccctccgcc gcgcgcaacg cccacaactg cctcagcttc
     1321 atctctatct cctcctgcga gggcacgcgg gtctgcacct ctaccaccca gcggcgcgcg
     1381 ccgagcaggg acatcagggc gcgcatcttg ggcacgtagg agagcgccag cgcgggcacg
     1441 cctgcgagcg ccccaaatat gagcgcgtgc aagcgcatgc tcaccagcat gtccgccccg
     1501 gcgatatagc ccgcgaggtc ggacggcgtg tgtcgcaccc ccgcccaggc aatcgcctcc
     1561 cccggcacgc ccagccgcaa cgcttcctgc tctacctgcc tcaacacctc gtcgtcgcgc
     1621 tccgcccaga acggcagcag caccactctc gcaccgaact cctccacgca gcaccgcatc
     1681 ccgtgcgcca ccgagcgcgc gaacctgcgg tcctcatcga gaccgggccg cgtctccagc
     1741 ttcaggcggt gtcgcacgcc cacgggcagc agatagcgcg ggttcaagcc tggactgtcg
     1801 tgcagcctgt gtcgcacgga gaatatgaca gtaggtttgt gccccgcgtc cccttccagc
     1861 ggaacgggcg ttggttcgac caggaaggcg gggtcggcgg tgacgcgcac ttgctcggca
     1921 cgcacgcctg ccgcccgtag cgctcgcgcc gaccccgtat cgcgcacgct gaagtaacgc
     1981 gacgacctca caacgaaccg ggcaagcaag cggcccagct tcgtgtagag cggccccacc
     2041 ccgaggcccc agccgaacac aggtatgccg cgcagcctgg caagggccac gtacaaggcg
     2101 tatagcggca ggttgtacac gctggagcta tcttgtatca ggccgccacc gccgagcacc
     2161 agggcttgcg attggaggac cgcgcggagg ggggtggggt cgagggggtt gcggggcacg
     2221 ctttgcacgt ggtaggtgct tgcggttgcg ttggggttag tggagagcac caggaggtcg
     2281 tcgtgcccgg cacggcgtag cgactccacc accgattgca gcaccgcctc gtccccggcg
     2341 ttcgcgctac cgtaccagcc gagaatggcg atcttcttgc gcttctgctg gccgccccta
     2401 ccgtccgcct tgccaggccc gcacatcatt cttgcctggc accaggggcc tcatgcggct
     2461 cggtctcggt ggccttaccg gacctggcac ccgcttgcga aggctggccc tggctctggc
     2521 cctggttgtg gccgtgcccg tcgtgtatca cccgcatgaa cagcctctgg tagcgccgcg
     2581 ccacgatgtc ccacgaataa tggccctgca cgtacttgcg ggctttgcgg ccaagcgcct
     2641 ctcgctgctc gtcgtcggcc agtagcgcga ggatgttatc cgcgaaggac tcgctgtcga
     2701 gcgggtcgag caggataccg ttctggcccg gcacaatcgc ctccggtatg ccgtccagcc
     2761 gcgaagccac gacgtagcgc ccggcggccg ccgcctctag cgccaccaac ccaaagccct
     2821 cgatgtcgtg ctgcacaggg atgttcggca tcacgaaaag gtcgcacagg tggtaggccg
     2881 cccgcagggt atcgtcgtcc acgcggccca cgagcaccac gtagtcttcc aggtgcagcg
     2941 aggctatgcg cgtctcgatc aactcgcgtt gcggcccctc gcccacgatg aggtaacaaa
     3001 tgtcctgccg ctgagcgatg attcggggca tcacctcggt gaggaagtgc accacgccct
     3061 tgcgctcgat gaggcggccc accgtgagga gcaccttacg gttgcgcaac ctgctaccgg
     3121 cggccatttg caggttgcgc attgcctggg gcgtgaactg gtccgcgaat tcgagcatgt
     3181 tcacgccggt gggcacgacc tcg
//
LOCUS       DASRWZ010000391         1966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1198297, whole genome shotgun sequence.
ACCESSION   DASRWZ010000391 DASRWZ010000000
VERSION     DASRWZ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198297"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="VFH60_06040"
     CDS             <1..1477
                     /locus_tag="VFH60_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6313377.1"
                     /translation="LPAIAALMERGCYGRLDTNATLFAGGVWPTFLTSSDVPWQGMYH
                     YKMWRQERMRCEVASDGWLHQHTFWEALDEKRRVAVIDAPMTIKPPRVANGIQLSGWG
                     THDMIVKGSHPEGLWGRLEKEFGRSAMPAELFGPQTASTLLRLRDDLLRQTEQMSRLA
                     PALLGREDWDLFFVVLGATHRGGHYLWDLSQIDSDTLNPAQRSTLEGALEEIYRACDD
                     AVGKLVERLPHGGRVMLFALHGMGQNLGWSDLAPDILDRIQGRGESAPKKGLLYGIKR
                     KLPWQLVRQVTTRLPQSVQERLVSTWSSGMYDWKQTRYFPLPMDLAGYFRVNLRGREP
                     EGIVEPGAEYEAVCAELEAALRGFRDVETGTSIVDKVYRQDDLAPPGAPYRDLLPDLL
                     VTWGQISAIECRAIRSERYGELRLDAAGRLPSGRAGNHTNRGWVVMSGEGIERGGQLG
                     NSHIMDLAPTLLSWLGVEAPATFQGKPMYVGSKDALTLNTL"
     gene            1531..1944
                     /locus_tag="VFH60_06045"
     CDS             1531..1944
                     /locus_tag="VFH60_06045"
                     /inference="COORDINATES: protein motif:HMM:NF022698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HET6313378.1"
                     /translation="MPDKDYSHRDVTDKLGIKPGSIVAFDEQAATLDEALRRKILERT
                     GCDTAGPGEPADVVLAPLHAGADAATLLRGWRSRIKANGGIWLLTPKRGQRGYVDQRE
                     LIAAGAEAGLVDNKSCSVDDTTSGLRFVIRKSDRA"
BASE COUNT          367 a          603 c          697 g          299 t
ORIGIN      
        1 gctcccggca atcgcagccc tcatggagcg tggctgctac ggaaggctcg acaccaacgc
       61 cacacttttc gcgggcgggg tgtggcccac cttcctcact tcgagcgacg tgccgtggca
      121 gggcatgtac cactacaaga tgtggcggca ggagcggatg cgttgcgagg tggcgagcga
      181 cgggtggctg caccagcaca ccttctggga ggccctcgat gagaagcgcc gtgtggcggt
      241 gatagatgcg cccatgacga taaagccgcc gagagtagcc aacggcatcc agctatcggg
      301 ttggggcacc cacgacatga tcgtgaaggg gtcgcaccct gaggggttgt ggggccgctt
      361 ggagaaggag ttcgggcgct cggccatgcc cgccgagttg ttcggccccc agaccgcgag
      421 cacgctgctg cgcctgcgag acgacctgct ccggcagacg gaacagatgt cgcggctggc
      481 ccccgctctg cttggcaggg aggactggga cctcttcttc gtggtgctgg gggccacgca
      541 ccggggcggg cactacctgt gggacctgtc gcagatagat agcgacacgc tcaaccccgc
      601 ccagcgcagc acgctggaag gtgccctgga agagatatac cgggcgtgcg acgacgcggt
      661 ggggaagctg gtcgagcggt tgccacacgg ggggagggtc atgctgttcg cgctgcacgg
      721 tatggggcaa aacctgggct ggtcggacct tgccccggac atcctcgacc gcatccaggg
      781 caggggagaa agcgcgccga agaaggggct gctgtacggt atcaagcgca agctgccgtg
      841 gcagttggtg cggcaggtga cgacccggct gccgcagagc gtgcaggagc ggctggtatc
      901 gacatggtcg tcgggcatgt acgactggaa gcagacccgc tacttccccc tgcctatgga
      961 cctcgccggg tacttccgcg tgaacctgag gggccgcgag ccggagggca tcgtggagcc
     1021 gggggccgaa tacgaagcgg tgtgcgccga gcttgaagcg gccctgcgcg gcttccgcga
     1081 cgtcgaaacg ggcacgtcca tcgtggacaa ggtataccgg caggacgacc tcgccccgcc
     1141 cggtgcgcct tacagagact tgctgcccga cctcctggtg acgtgggggc aaatctcggc
     1201 catagagtgc agggccatac gctcggaacg ctacggagag ctgcgcctgg acgccgcggg
     1261 taggctgccg tcgggccggg cgggcaacca cacgaataga ggctgggttg tcatgtcggg
     1321 cgagggtatc gagcgtggcg ggcagcttgg caacagccac ataatggacc ttgcccctac
     1381 cctgttatcg tggctggggg tggaggcacc cgccactttc cagggcaagc ccatgtacgt
     1441 tggaagtaag gacgcgctga cactcaacac actctagccg ggggttaccc tcgtgattgc
     1501 cacctggccc ggccgattga cgacacaaca atgcccgaca aagactacag ccaccgggac
     1561 gtgacggaca agctaggcat caagcccggc agcatcgtcg cgtttgacga gcaagcggca
     1621 acgctggacg aggccctccg gcgcaaaatt ctggagcgga ccggctgcga cacggccgga
     1681 ccgggtgagc ctgccgacgt ggtgctggca ccgctacacg ccggtgcgga tgccgcgacg
     1741 ctgctccgag ggtggcggtc ccgcataaag gcgaacggcg gcatctggct gcttactccc
     1801 aagcgcggcc aacgcggtta cgtcgatcaa cgcgagctaa tcgcggcagg ggcagaggcg
     1861 ggcctggtgg acaacaaatc gtgctcggtg gacgacacca cgagcggcct gcgcttcgtc
     1921 atccgcaaaa gcgaccgcgc ataagaaccg ggatgcggtt gccctc
//
LOCUS       DASRWZ010000392         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_307487, whole genome shotgun sequence.
ACCESSION   DASRWZ010000392 DASRWZ010000000
VERSION     DASRWZ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307487"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFH60_06050"
     CDS             complement(<1..364)
                     /locus_tag="VFH60_06050"
                     /inference="COORDINATES: protein motif:HMM:NF025390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4240 domain-containing protein"
                     /protein_id="HET6313379.1"
                     /translation="MDVTPFWRLIGEATAESGGECEREVELLRDKLMALSPEDIVEFH
                     NILTQFRRQAYSHDLWAAAYIINGGCSDDCFDYFGAWLIAQGEEVYSNTLRDPETLAE
                     VARFEQVECESLLSVAAAK"
     gene            complement(382..2523)
                     /gene="tkt"
                     /locus_tag="VFH60_06055"
     CDS             complement(382..2523)
                     /gene="tkt"
                     /locus_tag="VFH60_06055"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET6313380.1"
                     /translation="MDIDTLCINTIRTLSMDGVQQANSGHPGTPMADAPLAYLLYNEV
                     LRHNPRDPHWPNRDRFILSAGHASMLLYSALYLSGYDLSLEELRNFRQWGSKTPGHPE
                     YSHTAGVETTTGPLGQGFGNGVGMALAERWLAERFNKPGFDIVDHYTYALCSDGDLME
                     GVASEAASIAGFLKLGKLIYFYDDNTITIDGHTDLAFSEDVGKRFEAYQWHVEHVDDI
                     NDLDKLRKAIEAAKAETDRPSLIVVKTIIGYGSPNKADTSEAHGSPLGKEEIALSKQN
                     LGWPGEETFYVPDEALQHMRLAGPRGEQAQQEWDELFARYAQEFPEAAAEFKLFLSGG
                     LPDGWEEAIPSFPPEKGPMATRTASGAVINAIAPKILNLVSGAADLYPSTDAYIKGGG
                     DLKGDNFQARNVHYGIREHGMGSVLNGITLHKGLIAFGSTFLIFYDYMRPPLRLAALM
                     KAPTLMVYTHDSIGLGEDGPTHQPVEMLSGMRAVPNLWAVRPADANETAYAWRVALTR
                     RDGPTCIMLSRQKLPIFDRNEVGSAEGVLRGAYILADAEGGEPDVILMGTGAEVQWAL
                     KARPILQEQGIKARVVSMPCWELFEEQDQSYRDEVLPPHIKKRVSIEAASPMGWQKWV
                     GDEGSIIAVETFGASAPAEIIFEKYGFSTENVVEHALALMGRRGPVPPAQTEQEVTPD
                     LGEGGQAPHERELERNGPTETSQETPAEKQT"
BASE COUNT          488 a          898 c          952 g          611 t
ORIGIN      
        1 gcttcgcggc tgccacggaa agcaagctct cgcattctac ttgttcaaac cgggccacct
       61 ctgccagagt ctccgggtcg cgtaacgtgt tgctgtatac ttcctcgccc tgcgctatca
      121 gccaggcgcc gaagtagtcg aagcagtcgt cggagcagcc gccgttgatg atgtaggccg
      181 cggcccagag gtcgtgtgag taggcttgcc tcctgaactg ggttagtatg ttgtgaaact
      241 cgactatatc ttcgggcgag agggccatca atttgtctct caggagttct acctccctct
      301 cgcattcgcc cccgctttcc gctgtcgcct ctccaattaa tctccagaac ggagttacat
      361 ccatcctcaa ctccacctaa atcacgtttg cttctccgcc ggggtctcct gcgacgtttc
      421 ggtggggccg ttgcgctcta gctctcgctc gtgaggagcc tggccgcctt ctcccaggtc
      481 gggggtgact tcctgctcgg tctgggcggg gggcaccggc cctcggcggc ccatgagcgc
      541 gagggcgtgt tccaccacgt tctcggtgct gaagccgtac ttctcgaaga ttatctctgc
      601 cggggccgac gcgccgaacg tctccaccgc gatgatgctg ccctcgtcgc ccacccactt
      661 ctgccagccc atcggggagg cggcttcgat tgagacccgc ttcttgatgt gaggcggcag
      721 cacctcgtcc cggtaagact ggtcctgctc ctcgaatagc tcccagcacg gcatcgacac
      781 cacgcgggcc ttgatgccct gctcctggag gataggccgc gccttgagcg cccactgcac
      841 ctccgcgccg gtgcccatca gtatcacgtc aggctcgccg ccctccgcgt ctgctaatat
      901 atacgcgccg cgcaacacac cctcggcgga gcctacttcg ttgcggtcga agatgggcag
      961 cttctggcgg ctgagcatga tgcaggtggg gccgtcgcgc cgtgtgaggg ccacgcgcca
     1021 ggcgtaggcc gtctcgttcg cgtcggcggg gcgcaccgcc cacaggttgg gcaccgcacg
     1081 catgcccgac agcatctcca ccggctggtg ggtggggccg tcctcgccca ggccgatgct
     1141 atcgtgggtg tagaccatca gcgtgggggc cttcatcagc gcggcgaggc gcaggggggg
     1201 ccgcatgtag tcgtagaaga tgaggaaggt cgagccgaag gcaatgagac ccttgtgcag
     1261 ggtgatgccg ttgagcaccg accccatgcc gtgctcgcgg atgccgtagt ggacgttgcg
     1321 ggcctggaag ttgtcgcctt tcaggtcgcc cccgcccttg atgtaggcgt cggtggaggg
     1381 gtagaggtcg gcggccccgc tcacgaggtt gagaatcttg ggggcgatgg cgttgatgac
     1441 cgcgcccgaa gcggtacggg tggccatcgg acctttttcg ggcgggaagc tggggatggc
     1501 ctcctcccag ccgtcgggca ggccacccga taggaacagc ttgaactcgg cggcggcctc
     1561 ggggaactcc tgcgcgtagc gggcgaaaag ctcgtcccac tcctgctggg cctgctcccc
     1621 gcgcggccct gccagccgca tatgctgtag cgcctcgtcg ggtacgtaga aggtctcctc
     1681 cccgggccag ccgaggtttt gcttggagag ggctatctct tccttgccga gcgggctgcc
     1741 gtgcgcctcc gaggtgtcgg ccttgttggg cgagccgtag ccgatgatcg tcttcacgac
     1801 gatgagcgag ggcctgtccg tctccgcttt ggccgcctct atggccttgc gcagcttatc
     1861 caggtcgttg atgtcgtcaa cgtgctccac gtgccactgg taagcctcga agcgcttgcc
     1921 aacgtcctcg ctgaacgcca ggtcggtgtg gccgtctatt gtgatggtgt tgtcgtcgta
     1981 gaaatagatc agcttgccca gcttgaggaa gcccgcgatg gaggcggctt cggaggccac
     2041 gccttccatc aggtcgccgt ctgagcagag ggcgtaggtg tagtggtcca cgatgtcgaa
     2101 gccgggcttg ttgaagcgct cggcgagcca ccgctcggcc agggccatgc ccacgccgtt
     2161 gccgaagccc tggccgaggg ggccggtggt ggtctcgacg cccgccgtgt ggctgtactc
     2221 cggatggcca ggcgtcttgg aaccccactg gcggaagttc cgcagctcct caaggctcag
     2281 gtcgtagccg gagaggtaga gggccgagta gaggagcata gaggcatggc ccgccgagag
     2341 gatgaagcgg tcgcggttgg gccaatgggg gtcgcgcggg ttgtggcgca gcacctcgtt
     2401 gtagaggagg taagccaggg gcgcgtcggc catgggcgtg cctgggtggc cggaattggc
     2461 ctgttgcacg ccgtccatag acagggtgcg gatcgtgttg atgcagaggg tgtctatatc
     2521 cataccgtaa taatcggtct taccctcttg agtagctacg tttgtcacag ctatattctc
     2581 ctgtgtccaa ggttgcgggt attccaatgt acccctaccg gtacagcacc atatgtgcca
     2641 acacaccgga cgcaaaaaag agggcgcggc taagagcctt ctatgaaggt tgctatgatt
     2701 ttgaggtgcg ccgactagca gctatctcaa gaacaagaca gtcaaatacc agcacccatg
     2761 ctccccgctt gtcatttcga acgtagtgag aaatctcgtc cttcagcaca ttgttccacc
     2821 tttcctatgc ggcagaatct tgcggcagaa tctcaggaga aaatggccac accggggtag
     2881 gggacgagat ttctcacttc gttcgaaatg acataggcgg ggcctgggtg ccggtattct
     2941 aatgcgtca
//
LOCUS       DASRWZ010000393         1730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_444504, whole genome shotgun sequence.
ACCESSION   DASRWZ010000393 DASRWZ010000000
VERSION     DASRWZ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444504"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1730
                     /locus_tag="VFH60_06060"
     CDS             <1..>1730
                     /locus_tag="VFH60_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313381.1"
                     /translation="PDTAIRLDSITDRFMWRLAYRKFPTHDSVVVNHTVDAGGDRAGV
                     RWYEIRNLNTAPTVQQQGTYAPADGLHRWMGSIAMDGAGNMAAGYSVSSSTVYPSIRY
                     AGRLTTDPPGTLPQAEESLMVGGGSQTETIAGRWGDYSSINLDTDDCTFWYTTEYYSV
                     TALRSWRTRIGSFKFPNCTPAPAATTTPTRTGTPPTAVPTGTATTMPTASATACAGGI
                     NTTGVITNTDPVQTGRLGRSAAISSCAVPKTCPAVGDTLVRHYDSYPFTNNSGTSQCV
                     TVRVINQCGNNSLLIAAYLGTFDPNNLCTNYLADMGLSGPDFTYSFNVPNGASYVVTV
                     LENSPNVGCANYNIRVSPCDVGTPLPTVTGTPPTATPTITPVPPTVTRTATAVVTPSA
                     TPCSVTTLLNEGFETGTLGTFTRTTTLGTFPWTVVTGTVASGTFAAHVDDPGEVSDQQ
                     LTQVNAVTIPAGATSAQLVFSHTYSFEDATDPWDGGVLEYSTTGTVWNDAGSLITQGG
                     YTGVITVTAGNPLSNRPAWVHVKPGFPAFSTVTANLMSLAGQSVRFRVREGSDQATGA
                     PGWNVDNIRI"
BASE COUNT          349 a          623 c          484 g          274 t
ORIGIN      
        1 acccgacacc gccatcaggt tggatagtat caccgaccgc ttcatgtgga ggttagccta
       61 ccgcaagttc cccacacacg actcggtggt ggtcaaccac acggtagacg caggtggcga
      121 ccgcgcgggc gtcaggtggt acgagatacg caacctgaac acagcgccca ccgtgcaaca
      181 gcaaggcacc tacgccccag ccgacggcct gcacaggtgg atgggcagca tcgcgatgga
      241 tggcgcgggc aacatggccg caggctacag tgtgagtagc agcaccgtct atccctccat
      301 ccgctacgcc ggccgcttga ctaccgaccc acccggtacg ctgccgcagg ccgaggagag
      361 cctcatggtg ggcggcggct cccagaccga gacgatagcc gggcgctggg gcgactacag
      421 cagcatcaac ctcgacacgg acgactgtac cttctggtat acaaccgaat actactcggt
      481 cacggccctg cgctcgtggc gcacgcgtat cggctcgttc aagttcccca actgcactcc
      541 ggcacccgcc gccaccacta ctcctactcg caccggcacg cctcctaccg cagtcccaac
      601 aggcacggcg acaaccatgc ctaccgcttc cgccaccgca tgcgccgggg gtatcaatac
      661 gacaggcgtt atcaccaaca ccgacccggt gcagaccgga cgcctggggc gcagcgccgc
      721 tatcagcagt tgcgcggtcc ccaagacctg cccggcggta ggcgatacgc tcgtgaggca
      781 ttacgactcc taccccttca ccaacaacag cggcaccagc cagtgcgtga cggtgagggt
      841 catcaaccag tgcggcaaca actccctgtt gatcgcggct tacctcggca ccttcgatcc
      901 taacaacctt tgcaccaact acctggccga tatgggcctg tcgggacccg acttcaccta
      961 ttcgttcaac gtgcccaacg gggcgagcta cgtggttaca gtcctggaga actcgcccaa
     1021 cgtcggctgc gctaactaca atataagggt cagcccttgc gacgtaggca ccccgctgcc
     1081 caccgtgacg ggcacgccgc ccacggctac ccctaccatc acgccggtgc cacctacagt
     1141 cacgcgcacg gctactgccg ttgtcacccc ctcagctacg ccatgctccg tgacgacact
     1201 actcaatgag ggcttcgaga cgggcacgct cggcactttc acccgcacca ccaccctggg
     1261 tactttcccc tggacggtcg ttacgggcac ggtagcgtca ggcacgttcg cggcccacgt
     1321 agacgacccc ggcgaggtat cggaccagca actcacgcag gtgaacgccg tgaccatacc
     1381 cgcaggcgcg acttcggcac agctcgtctt ctcgcatacc tacagcttcg aggacgctac
     1441 ggatccatgg gatggcggcg tgcttgaata ctccaccacc ggcactgtct ggaacgatgc
     1501 agggtcgctc atcacccagg gcgggtacac cggcgtgatc accgtcaccg ccggcaaccc
     1561 gctgtccaat agaccggcgt gggtacacgt aaagcccggc ttcccggctt tcagcacggt
     1621 gacggcgaac ctcatgtcgc tcgcgggcca gagtgtgcgc ttccgcgtcc gcgaaggcag
     1681 cgaccaggcc accggagcgc ccggctggaa tgtggacaac atcaggatca
//
LOCUS       DASRWZ010000394         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1335132, whole genome shotgun sequence.
ACCESSION   DASRWZ010000394 DASRWZ010000000
VERSION     DASRWZ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335132"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="VFH60_06065"
     CDS             <1..1332
                     /locus_tag="VFH60_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313382.1"
                     /translation="RLPAWGLTGGGGLSILGANPLYWLLHGNREGSKMGRVLFALSLV
                     ACTLLLNACGVGGPPQPTPTKLEHDVDQVKLGVEDVRELSAKHDVPLRLVSTEELGEA
                     HAKVLERYYSREEGLRESLELWLLRLIEDRSVDLYQLSKDLFTQGYVGFYVYDTKEMF
                     VLSEGNELVAEPRAILAHEFAHALQDQHFDLAKLEPPDSDDYDRNLAISALIEGDAQW
                     TTGLYVQSYMPSGDLLKLKYAAVPVPPDDSPRFPYYLGELGSFPYEWGEAFVGEVLKR
                     DLYKFEAVNKVFAAPPSSTEQIMHPEKYLDTRDDPLPVTVPPLTNTLGTGWTYEHGAS
                     IGEFSLQRLLAYHYSNDPEGEAAGWGGGRYDLYTKGEDALLITVTRWDTSEDAVEFHE
                     ALLVTFLDYEHEGDIYDDGLGRFFTAGLDGDTVTFTASTDRAALERIAGGR"
     gene            1722..>2913
                     /locus_tag="VFH60_06070"
     CDS             1722..>2913
                     /locus_tag="VFH60_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313383.1"
                     /translation="MRHKTRAFLMSVCLLVLAACNSTGLPVSPTNTPQLNTQATPTAE
                     SVAGAPSATPRNQARPTRTPRTAQATEAPTDDATAEPTEDAGPQPTSEASNTKLDKEV
                     MEVERNTANVRGLAPKGDVPETFLTQPQMKSNLLNDITEDYSREEANEDAMELWLLRL
                     MDDPETDLLQLHIDLLGEQVLGYYDPEEDEMFVLRNQADLSPGSKQTLAHEFVHALQD
                     QYFDLEKMLPDESHDDDRSLAIRAVVEGDATMAGLSYVRDYFTQADIEEFLQESSSAD
                     TSVLNSAPAYLRESLYFPYDAGADFVNELLMMDGFQAVDEALADPPASTEQILHPDKY
                     FDSPRDEPIQVPLPPLTDTLGAGWTMVDWGTLGEFDLRVILDENGAADPDEAAAGWGG
                     GSYAF"
BASE COUNT          591 a          946 c          869 g          507 t
ORIGIN      
        1 cgtctcccgg catggggctt gaccgggggc ggggggcttt ctatacttgg tgcgaaccct
       61 ttgtactggc tgctgcatgg aaacagggag gggagtaaga tgggccgcgt gctgtttgct
      121 ctgtcgcttg tggcttgtac actgctcttg aacgcttgcg gggtgggcgg gccgccgcag
      181 cctacgccca ccaagctcga gcatgacgta gaccaggtga agctgggcgt ggaggacgtg
      241 cgcgagttaa gcgccaagca tgacgtgccc ctgcgcctgg taagcacgga ggagttggga
      301 gaggcccacg caaaggtcct ggaacgctac tactcgcggg aggaagggct gagagagtcg
      361 ctggaactct ggctgctgcg cctcatcgaa gacaggtcgg tcgacctgta ccagctcagc
      421 aaggacttgt tcacccaggg ctacgtcggc ttctacgtgt acgacacgaa ggagatgttc
      481 gtgctaagcg agggcaacga gctggtagcg gagcctcgcg ctatcctggc ccacgagttc
      541 gcccacgccc tccaggacca gcatttcgac ctggcaaagc tggaaccccc cgacagcgac
      601 gactacgacc gcaatctggc catctccgcg ctgattgagg gcgacgcgca gtggactacc
      661 ggcctctacg tacaaagcta tatgccctcc ggggacttac tcaagctgaa atatgccgcc
      721 gtacccgtgc cgccggacga cagccccagg ttcccctact acctgggcga attaggctcg
      781 ttcccgtacg agtggggcga ggccttcgtg ggcgaggtgt tgaagcgcga cctgtacaag
      841 ttcgaggctg taaacaaggt cttcgcagcc ccaccctcct ccaccgagca aataatgcac
      901 ccggaaaagt acctcgacac gcgcgacgac cccctgcccg tcaccgtgcc gcctctcaca
      961 aacacgctcg gcaccggctg gacctacgag catggcgcga gcataggcga gttcagcctg
     1021 caacgcctac tcgcttacca ctactccaac gaccctgagg gtgaagccgc aggatgggga
     1081 ggcgggcgct acgacctcta caccaaaggc gaggatgcgc tgctcattac cgttacccgg
     1141 tgggacacgt ccgaagacgc tgtggagttc catgaggcgc tactggtcac gttcctggac
     1201 tacgagcacg aaggggacat ctacgacgat ggcctgggcc gcttcttcac cgccggcctc
     1261 gacggcgata cggtcacctt caccgcctcc acggaccgtg ccgccctcga acgcattgcc
     1321 gggggcaggt aacggcagac cacaggctca gggtgcagcc attctcggct ggtagggcag
     1381 gctctgtgga gaacccctgc ggggacgccc tgcatggacg ccctgtgggt tatccaaggg
     1441 cgtcccgcaa ggctccccac caccttcctg ccgcattgct acaccgtggc agaagcaggc
     1501 tatccttgac tccagtaccc ccagtatcta tacttcaagt gcaagagcgt ccgcggcgct
     1561 cgttgcaggt agaagccaaa gaacccttcg ccgagcgcgc aggtatacat atatagatgc
     1621 gaatacggcg cgcccttccc gctattattc tctcaaacta cgccagtttt cacccgtccc
     1681 ggcttcttac cgcatagctt cccctttaga taggagtaac catgcgccac aaaacgcgcg
     1741 cttttctgat gtcggtatgc ctgctggtgc tggctgcctg caacagcacc ggcctccccg
     1801 tatcacctac caacacgccg caactcaaca cgcaagccac ccccaccgct gagtcggtgg
     1861 caggtgcccc atccgcgacc ccccgcaacc aggcccggcc cacgcgcacc cctcgtaccg
     1921 cacaagccac cgaagcaccc acggacgatg ccacggcgga gcccaccgag gatgccggtc
     1981 cgcagcctac ctccgaggcg agcaatacga agctggataa agaggtgatg gaggtcgagc
     2041 ggaacaccgc caacgtccgg ggcctcgctc ccaagggcga cgtgcctgag actttcctca
     2101 cccagcccca aatgaaaagc aacctgctca acgacattac cgaggactac tctcgcgaag
     2161 aggccaacga ggacgcgatg gagctatggc tgctgcgcct catggacgac cccgaaacgg
     2221 acctgttgca gttgcatata gatttgcttg gcgagcaggt gcttggctac tatgaccccg
     2281 aagaggacga gatgttcgtg ctgcgcaacc aggcggacct cagccccggc tccaagcaga
     2341 ccctggccca tgagttcgtg cacgctttgc aggaccagta cttcgacctg gaaaagatgc
     2401 tgcccgacga aagccacgat gacgaccgct cgctggcgat acgggcagta gtcgagggtg
     2461 atgccaccat ggccgggttg agctacgtgc gcgactattt cacgcaggcg gatatagagg
     2521 agttcctcca agaaagctcg tcggccgaca cgtcggtgct gaatagcgcc cccgcgtacc
     2581 tgcgcgagag cctctacttc ccctacgatg ccggtgccga tttcgtgaac gagttgctga
     2641 tgatggacgg cttccaggcg gtagacgaag cgctggccga cccgcctgct tccaccgagc
     2701 agatactgca ccccgacaag tacttcgact cgccacgtga cgagcctata caggtgcctc
     2761 tgccgcccct caccgacacg ctgggcgcgg gctggactat ggttgactgg ggcacgctgg
     2821 gcgagttcga cctgagggtg atcctggatg aaaacggcgc tgccgacccc gatgaggctg
     2881 cggcgggctg gggtggcggc agctatgcct tct
//
LOCUS       DASRWZ010000395         1984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_238639, whole genome shotgun sequence.
ACCESSION   DASRWZ010000395 DASRWZ010000000
VERSION     DASRWZ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238639"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFH60_06075"
     CDS             complement(<1..627)
                     /locus_tag="VFH60_06075"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET6313384.1"
                     /translation="MSKTQPREAADPGFEVEIKSGGRAYDAGAPRVAPSLSPARAAAK
                     AQARKRRLLIIALAVLALGSIMAWAWLSNPNSLVPPDAVARVNGEYIYERDINRELDL
                     TRAFNEVSQAQYNEAAELPSAAIVLEDLISRKMREQDARKAGVAVAPAEVDTELRAVL
                     DGVGMSEEELAAVFGKYNLKIDDLRGVLAGTALLRKYVDDYVVAGATSE"
     gene            complement(629..1420)
                     /locus_tag="VFH60_06080"
     CDS             complement(629..1420)
                     /locus_tag="VFH60_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET6313385.1"
                     /translation="MPTPDINLFWALIAGLASFLSPCVLPLVPAYIGYMGGSSVMAAR
                     GAVSGGGTAGVSTGSARWLAVSHSLVFVLGFTIIFVVVIGGLAGALSYFLQDNKRILQ
                     QVMGVLLVLFGLHMVGLINLPFLNYTRRMDIRPAQNLGYLRSLLIGMGFAIGWTPCIG
                     PVLSGIFSLALEGKQLEAFPLFFAYSMGLGIPFIITAMAMGQISAGLKKLTRHSYSFK
                     LGGWTVLKDVNIISLISGVLLIAMGVLIFTNSLTILAPSINWINL"
     gene            complement(1516..>1984)
                     /locus_tag="VFH60_06085"
     CDS             complement(1516..>1984)
                     /locus_tag="VFH60_06085"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HET6313386.1"
                     /translation="CHQLPERAGSVFGYQVAFCYRNTALYLGIAFFGLLFGLARDRDV
                     AWLRWLKRPAPWYVFVLLLLPMIIDGGSHMLGLRDTMSDMVEPTFNSFYIGSQIWSLN
                     WWLRVLTGAAAALGAVWFAFPRMERAMLEAETLRQMYKQAALRRQQKALAPNT"
BASE COUNT          393 a          585 c          639 g          367 t
ORIGIN      
        1 ctcgctggtg gccccggcga ctacgtaatc gtctacgtac ttccgtagca gcgcggtgcc
       61 cgctaatacg ccgcgcagat cgtcaatctt gaggttgtac ttgccgaaga cggcagcgag
      121 ttcttcctcc gacatgccga ccccatccag aacggctctt agttccgtgt caacttcggc
      181 gggcgctacg gccacgcctg ccttgcgcgc gtcctgctcg cgcatcttac ggctgatgag
      241 gtcctccagc actatcgccg cgctcggtag ttcggcggcc tcgttgtact gggcctggct
      301 cacttcgttg aaggcgcgtg tgaggtcaag ctcgcggttg atgtcacgct catagatgta
      361 ctcgccgttg acgcgcgcca ccgcgtcagg aggcaccagc gagttggggt tggagagcca
      421 cgcccacgcc atgatggagc ctagcgcaag gacggccaga gcgataataa gcagcctgcg
      481 cttgcgggcc tgcgctttgg cggcggcgcg ggcgggcgac agggaagggg cgacacgtgg
      541 cgctcccgcg tcgtatgcgc gcccgccgct ctttatctct acctcgaagc cgggatctgc
      601 cgcctcgcgt ggctgcgtct tgctcatttt acaggttgat ccagttgata gatggggcga
      661 gtatcgtcag cgaattagtg aagatgagca cgcccatcgc gatgagcagc acgccactga
      721 ttagcgagat aatgttgaca tccttgagga cggtccaccc tcccagcttg aaagagtagc
      781 tgtggcgcgt aagcttcttg aggcccgccg agatctggcc catcgccatc gcggtgatga
      841 tgaatgggat gcccagcccc atagagtagg cgaagaaaag ggggaacgcc tccaattgct
      901 tcccttcgag ggccagcgag aagataccgc tcagtacggg gcctatgcac ggcgtccaac
      961 caatagcgaa gcccatgccg attagcagcg acctcaaata gcccaggttc tgggcggggc
     1021 ggatgtccat gcggcgcgtg taattgagga acggcaggtt gatgaggccg accatgtgca
     1081 ggccgaagag caccagcagc acgcccatga cctgctgcaa tatgcgcttg ttatcctgga
     1141 gaaagtagct cagcgcgccc gccaggccgc ctatgactac gacgaatatt atcgtaaagc
     1201 cgagcacgaa caccaacgag tgtgataccg ccagccagcg cgccgagcct gtggataccc
     1261 cggctgtgcc gccgcctgac acggcccccc gtgccgccat cacggacgag ccgcccatgt
     1321 agccgatgta cgcgggcacc agcggcagca cgcacggaga taaaaacgag gccaggcccg
     1381 cgatcaaggc ccagaagagg tttatatcgg gtgttggcat actgtctcct gtgagtggtc
     1441 agtggtcagt ggtcagtggt cagtggtcag tgaccaggga ttaggggtca ggtgttgggt
     1501 gttgggtatt gggtgttagg tgttgggggc gagggctttt tgctgtcggc gtagggcggc
     1561 ctgcttgtac atttggcgta gcgtttcggc ctctagcatg gcacgctcca tacgcgggaa
     1621 tgcgaaccac accgcgccta atgcagccgc cgcgcccgtg aggacgcgca gccaccagtt
     1681 cagggaccat atctgcgagc cgatgtagaa cgagttgaaa gttggctcta ccatatccga
     1741 catggtgtcc cgcaggccca gcatgtgcga gccgccgtca attatcattg ggaggaggag
     1801 cagcacgaat acgtaccagg gtgcgggccg cttcagccag cgcagccacg caacgtcacg
     1861 gtcacgggcc aggccgaaca acagcccgaa gaaggcgatg ccgagataga gcgcggtgtt
     1921 gcggtagcag aaggccacct ggtagccaaa gacgctcccg gcgcgctccg gcaactggtg
     1981 gcag
//
LOCUS       DASRWZ010000396         7388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1130090, whole genome shotgun sequence.
ACCESSION   DASRWZ010000396 DASRWZ010000000
VERSION     DASRWZ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130090"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VFH60_06090"
     CDS             complement(<1..781)
                     /locus_tag="VFH60_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6313387.1"
                     /translation="MNLSQLPQQLLNGIFSGSIYALFALGYTLIFGVLDILNLAHAAI
                     FMLGATFAWWLVVAQGWPLWLAFAVAVLISGLLGVLLDRVAFLRLRLRGAPQLSALIS
                     SIGMALIFVGMARLLFNAERKQFPYTSFPAVFFDIGTLRIRLLDVIILAVSLAMMFGL
                     NYLVRRTSLGRAMRAVAENPKAAALLGVNIDRVIMQTFFIASALGGAAGILYGMSLNS
                     IEPRIGYQVELNGLSITIRDAMRTIAGAMIAGFLIGMVEVLS"
     gene            complement(938..2254)
                     /locus_tag="VFH60_06095"
     CDS             complement(938..2254)
                     /locus_tag="VFH60_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6313388.1"
                     /translation="MFSKRTRTGSLALSLLILFSLLLSACGGETAQPAATSTATTGQE
                     AAPTTAGGDQTPAGGTDAGATATTPAGTDATTTVPAGGGGGTLKVGFAFVTSGDNAVY
                     GNSQRAAAELAIEEINAAGEGPQIEGIFEDTAAKPDQAITVFQKFINNDQVHAIIGPT
                     LSNEARSSDPVAQTAGVPVLAVSNTAGGITDMGDFIFRDSLAEFQVIPETVRQAKEVL
                     NISKVSLLYASDDAFSKSGADVFRTELQKNNIQILSEQTFATADTDYRTQLTQIKGEN
                     PDAIVVSALANPAQTILQQARRDVGIDPKVHIIGGNGFNSPAVVKAAGDTAEGLVVGA
                     AWNLNATDELSQEFIKNFNAKNGRDPDQFAAQAYAGVYILHDAATRADIEGKPLAEAR
                     TAIRDALKSTDAVPTVLGPFSFTDKRDADHPPVVQIVKDGAFEILK"
     gene            complement(2402..3493)
                     /locus_tag="VFH60_06100"
     CDS             complement(2402..3493)
                     /locus_tag="VFH60_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6313389.1"
                     /translation="MEWATLGQPQGQIEGSPDRAEVLEQFHRRTQIRRMRELAEAALS
                     AYDLPRTRLTLLAHLFNTTFRVDTATGQRYVLRIHRAGTPTVDTVSAELAWLAALRRD
                     TPLEVPTPVPTTAGTLLTEATTPRLPRPHICVLFRWLPGRLLRRGLTPRYLERIGELM
                     ARLQDHAVRWGPPSGFARGRVDSPIDTARWLPDPFAPEVLTDIHALVADTLSEAEAAQ
                     VTAVLARVQAVQRALGQGADTFGLVHADLHYGNLLFAGGAVRPIDFDDCGFGPWLYDP
                     AVMLRPLLGREAYPALRAALLSGYRRVRPLPAEHEAHLDTFIALRQVQDAIWVLESRR
                     HPAIADDWATQARQYLASLPALLPDADIF"
     gene            complement(3503..3841)
                     /locus_tag="VFH60_06105"
     CDS             complement(3503..3841)
                     /locus_tag="VFH60_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313390.1"
                     /translation="MSETSNLNKLEARCSQVMSSPYLRKDTPPELKNLRSVLQTVSLL
                     ITEDLPPLITELRQLRSRNKKLEAELEALRSANEAAANGTAAEEEATGDVAAPDATDE
                     TATSEAPTQA"
     gene            complement(3931..4299)
                     /locus_tag="VFH60_06110"
     CDS             complement(3931..4299)
                     /locus_tag="VFH60_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6313391.1"
                     /translation="MAKKLYVGNLPYSVTEEQLREMFSQVGEISDAAVVTDRYTGQSK
                     GFAFVEMVSDEAAQAAIDKFDGQDMDGRAMVVNEARPREDRGGGGGRGGGGGYGGGGG
                     GRGGGDRRGGNGGGGGRGRY"
     gene            4843..6477
                     /locus_tag="VFH60_06115"
     CDS             4843..6477
                     /locus_tag="VFH60_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET6313392.1"
                     /translation="MATTTDNPATPRVSAAPAAPAVAARSRVAGLVLMLVVFIGGASV
                     MTVEMSASRLLAPYFGTSLFIWANLIGLVMVYLSLGYWLGGRLADRYPRASVLYTITA
                     IAGLAVSLIPSLSRPILSWSLTGFSERSVGIFYSSLVGVILLFSVPLLLLGFVSPFAI
                     RLRSVGVASAGGTAGQVSALSTLGSILGTFIPTFFLIPYIGTAATLYSAAAVLLAFSI
                     LGLLSTANTRQALVSAAMLLVVLGLAAFAPRGIVKPPERGQLIYEKESAYNYIQVVQE
                     GKRVALQLNEGHAIHSIYNPEQLLTGGPWDYWAVAPYFAEGFEPSDYKGMAMIGSAAG
                     TASHIITEAYGPIPMDGVEIDPEIVAVGSRYFALDTLPNVTSHVEDGRTYLQTNGRDK
                     KWTVIGIDAYRQPYIPFHLTTVEFFQEVKDHLTPDGSVMINAGRTLHDTRLVDALANT
                     MLQVYPNVYVIDVPTMANSMVIGTASPSRIENFAANAARIDNPVLKQIFDVSLEQGNI
                     REVKPDTAKLVFTDDKAPIEEVINQIILGYVGEAGR"
     gene            6523..7014
                     /locus_tag="VFH60_06120"
     CDS             6523..7014
                     /locus_tag="VFH60_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313393.1"
                     /translation="MNLMLRRSTRTATSEEVLIFDADNRDANGAAVNIGKIDVHFLDD
                     QVVGTLLLWNEFITGFDQAHGPQDDESLDVVIHQIMTEITDALGVPSEFGIEVYYPSV
                     QNYQFVSSYADDEAEDESDVADGGAYGGSSAVGLDTEYLRDEDEEPDANADDFVKRLR
                     ERP"
     gene            6968..>7388
                     /locus_tag="VFH60_06125"
     CDS             6968..>7388
                     /locus_tag="VFH60_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HET6313394.1"
                     /translation="MRTQTISSSDSASGRNPARPAGVPVRDGIYSVLALRPYTDLLHG
                     ISTRLSPDGTDWNLSARRGTPANPPDPEIALANRTGLAHRLGISLDRMVGCQQVHGNE
                     VALVGEEDAGKGMLPGRPALPDCDAMITATPGLYLLAL"
BASE COUNT         1353 a         2310 c         2310 g         1415 t
ORIGIN      
        1 cgctcagtac ctcgaccatg ccgatgagga agccggctat catagccccc gcaatggtgc
       61 gcatggcgtc ccggatggtt atggagaggc cgtttagctc gacctggtag ccgatgcgcg
      121 gctcgatgct gttgaggctc atgccgtata ggatgcccgc cgccccgcca agcgccgagg
      181 cgatgaagaa ggtctgcatg attacgcggt cgatattcac gccgagcagg gccgcagctt
      241 tggggttctc ggccaccgcg cgcatggcgc gccccaagga ggtacggcgg acgaggtagt
      301 tcagcccaaa catcattgcc aggctcacgg ccaggatgat aacgtccagc aggcggatgc
      361 gcagggtgcc gatgtcgaag aagacggccg ggaaagaggt ataggggaac tgcttgcgct
      421 cggcgttgaa tagcagccgc gccatgccca cgaagatgag ggccatgcca atcgagctaa
      481 tcagcgccga gagttgaggc gcgcccctca gccgcagccg caggaacgcc accctgtcca
      541 gcagcacccc cagcagcccc gaaatgagca ccgcgaccgc gaaagccagc cacaagggcc
      601 acccctgggc tacaacgagc caccacgcaa aggtcgcccc cagcatgaaa atagccgcgt
      661 gcgccaggtt cagtatgtcg agcaccccaa agatgagcgt gtacccaagc gcgaagagcg
      721 cgtagatact gcccgaaaag atgccgttca gtagctgctg tggaagctgt gagaggttca
      781 tgggtacggg gatagagtaa tgagtgatga gtgatgagga ctgagtaagt aggtgctgta
      841 agctacacgc agacgccaaa caatcctaaa ccttgcgccc tttgcgtttt cgtctcctac
      901 tcagtcctca gtcctcatca ctcattactc gtatcatcta cttcaaaatc tcgaatgcgc
      961 cgtccttcac tatctgtact accggcgggt ggtcggcgtc gcgcttgtcg gtgaaggaga
     1021 aggggccgag cacggtgggc acggcatcgg tgctcttgag ggcgtcgcgt atcgcggtgc
     1081 gggcctcggc cagcggcttg ccctcgatgt cggcgcgggt ggcagcgtcg tgcaggatgt
     1141 acacgcccgc gtatgcctgg gccgcgaatt ggtccgggtc cctgccgttt ttggcgttga
     1201 agttcttgat gaactcctgg ctaagctcgt cggtggcgtt gaggttccag gcagcgccca
     1261 ctaccaggcc ttccgcggtg tcgcccgccg ccttcacgac cgccggtgag ttgaacccgt
     1321 tgccgccgat gatatgcacc ttcgggtcga tacccacgtc gcgccgcgcc tgctgcaata
     1381 tcgtttgagc ggggttggcg agggcggata ccactatcgc gtcggggttc tcacctttga
     1441 tttgggtaag ttgggttctg taatcggtgt cggcggtggc gaaggtctgc tcgctgagaa
     1501 tctggatgtt gttcttttgc agctcggtgc ggaagacgtc agcgcccgac ttactgaagg
     1561 catcgtcgct ggcgtacagc agggagacct tgctgatatt cagcacttcc ttggcctgcc
     1621 tgaccgtttc gggtatcacc tggaactcgg ccagcgagtc gcggaagatg aagtcgccca
     1681 tatcggtgat gccgcccgcc gtgttggaga ccgccagcac gggcacgccc gccgtctggg
     1741 cgacagggtc ggacgaccgg gcctcgttgc tcagggtggg gcctatgatg gcgtggacct
     1801 ggtcgttatt gatgaacttc tggaagacgg tgatagcctg gtcgggcttg gctgccgtgt
     1861 cctcgaatat gccttcaatc tggggacctt cgcccgcggc gttgatctcc tctatcgcaa
     1921 gctcggcggc ggcgcgttgc gagttgccgt acacggcatt atcgccgctg gtcacgaacg
     1981 cgaagccgac cttgagagtg ccgccgcccc cgcccgccgg gacggtagtc gttgcatccg
     2041 tgcctgcggg ggtagtagcg gtcgccccgg catcggtgcc gcccgccggg gtctggtcgc
     2101 caccggctgt ggtgggcgcg gcctcctgcc ccgtcgttgc ggtcgaggta gcggcgggct
     2161 gtgccgtttc gccgccgcac gcgctcaaca gcaggctaaa caagatcaag agagacaacg
     2221 ctaaagagcc ggtgcgagta cgtttcgaga acatagctgg ttcctccttg tacgctggaa
     2281 gccgggaccg ttacaacgca gagtgccggt cttgcccatc cgcccggcac gtgggtgctc
     2341 tattttagca ccagcgaccg cttgcagcaa ctgcaggtaa taaagtgcag ggcgaatgca
     2401 actaaaagat gtctgcgtcg ggcagaagcg ccggtagcga ggccaggtat tgacgcgcct
     2461 gtgttgccca atcgtccgcg atagccgggt gcctgcgcga ctccagcacc cagatggcgt
     2521 cttgcacctg gcggagggca atgaaggtgt cgaggtgtgc ttcgtgttcg gcaggcagcg
     2581 gtcgcactcg ccggtagcct gagagcagcg cggcccgcag cgcggggtac gcctcccggc
     2641 ctaacaacgg cctcagcatc accgccgggt cgtacagcca cgggccgaag ccacagtcat
     2701 cgaaatctat gggccgtacc gcgcccccag cgaagaggag gttcccataa tggaggtcgg
     2761 cgtggaccag cccgaacgtg tctgccccct ggcccaacgc gcgctgcaca gcctgcaccc
     2821 gcgccagcac cgctgtcacc tgtgccgcct cggcctccga gagcgtgtct gccaccaggg
     2881 catgaatgtc agtcaagacc tccggcgcga acgggtcggg cagccaccgc gcggtatcga
     2941 tgggtgagtc tacccggcca cgggcaaaac cggaaggcgg tccccaccgg acggcatggt
     3001 cctgcaggcg cgccattagc tctccgatgc gctcaaggta gcgtggggtg agtccgcggc
     3061 gcagaagccg gcccggcagc cagcgaaaga gcacacagat gtggggccgg ggcaagcgag
     3121 gcgtggtggc ctcggtgagg agagtaccgg cggtggtagg taccggggtg ggcacttcca
     3181 ggggcgtatc acgccgtaaa gcggccagcc aggccagttc ggcactcaca gtatccactg
     3241 ttggcgtgcc cgcacggtgg atgcgtagga cgtagcgttg ccccgtagcg gtgtctaccc
     3301 ggaaggtggt gttgaataaa tgagccagca gggtaaggcg cgtccggggg agatcatacg
     3361 cggacagcgc tgcctcggcc agttcccgca tccgccttat ttgagtgcgc cggtgaaact
     3421 gctcaagtac ttcggcgcgg tcggggctgc cctctatctg tccttgtggc tgccccaacg
     3481 tcgcccactc catttcgcgc ggctatgcct gggttggcgc ctcgctcgta gccgtctcgt
     3541 cagttgcgtc gggtgctgcc acgtcgccgg tcgcctcttc ctcggcggca gtaccgtttg
     3601 ctgccgcttc gttggccgag cgtagggctt ccaattcggc ctcaagcttc ttgttgcggc
     3661 tgcgcaactg ccgcagctcg gtaatgagtg ggggcaagtc ctcggttatc aagaggctca
     3721 ccgtctgcaa cacgctgcgc aggttcttta gctccggcgg cgtgtctttg cgcaggtagg
     3781 gcgaactcat cacctggctg caacgcgctt ctagcttatt caagttgctg gtttcggaca
     3841 tagtgcttct cgcttcctca tgatgggctt aaatgccgag aggccaacca tgtgacctcc
     3901 cggcacttgt tgtagccgag caacaaataa ctaatagcgt cctcgaccgc cgccaccgcc
     3961 gttaccgccg cgcctgtctc caccgccacg gcctccgcca ccaccgccgt agcctccccc
     4021 gccaccgcgg ccaccgccgc ctcctcggtc ctcgcgcggc cgggcctcgt tgacgaccat
     4081 ggcgcgtccg tccatgtcct gcccgtcgaa cttgtcaatg gcggcctgcg ccgcctcgtc
     4141 gctcaccatc tcgacaaacg cgaaaccctt tgactgcccg gtgtagcggt cggtcacgac
     4201 ggcggcgtcg gagatttcgc cgacctggga gaacatctcg cgcagttgtt cttccgtcac
     4261 cgagtagggt agattgccca cgtaaagctt cttagccatt gttgcctcct attgtcaacg
     4321 aaccgcactc aagataggac atgtaatcgc gagaaaaagg gacaagccgc tgcttcccag
     4381 gcggtggtcc aggagggcgc tctgaagctc gaacgtaagg caatctcata acctatccca
     4441 tctactatag catacacttc cagtgcaagt attttgagca ttgcggctac accaatatag
     4501 taatagacga aaaccgcaaa ggcgcaaaga gcgccaagaa agcgcaaaga gaagaacggt
     4561 ttcgcaagtg caatgggaac agggtaggtc atgtgtgcgc tggtcctttt gcttagacca
     4621 cctactttat cgttccgtgt cctttgcgcc cttcgcgcct ttgcggtttc tccccttctc
     4681 atgttgctat cgttcccaag ctccaatttc gggatgtctg ctgcctgtta gggtttctta
     4741 gctggggcgt tcttgccgcg tggttcgaca gcacggtata atggccgggc ggcctgcgaa
     4801 tgcgccctat cgtagcgacc gcaattagtc aggagcaacc atttggcaac tactactgat
     4861 aaccctgcca cgcctcgcgt gagcgccgcg cccgccgccc ccgccgttgc ggcccgcagc
     4921 cgggtggccg ggctggtgct gatgctcgtg gtgttcatcg gcggcgcgtc ggtcatgacg
     4981 gtcgagatgt cggcctcgcg cctgctcgcg ccctatttcg gcacctcgct gttcatctgg
     5041 gccaatctga tcggcctggt gatggtctac ctctcgctcg gctactggct cggcggcagg
     5101 ctggccgacc gctacccccg cgcctcagtg ctctacacca tcacggcgat agcagggctg
     5161 gcagtgagcc tcatacccag cctgtcgagg ccgatactga gctggtcgct aaccgggttc
     5221 agcgagaggt cggtgggcat cttctatagc tcgttggtcg gtgttatcct gctcttctcg
     5281 gtgcccctgc tgctgttggg gttcgtgtcg ccgttcgcca taaggctgcg gtcggtgggt
     5341 gtcgcctcgg cggggggtac tgcggggcag gtgtcggccc tctccacgct tgggagcatc
     5401 ttgggcacct tcataccgac cttcttcctg ataccgtaca ttggtacggc agccactctc
     5461 tattcggctg ccgccgtgct gcttgccttc tccatactcg gcctgcttag caccgctaac
     5521 acccggcagg cgctcgtctc ggcggcgatg ctgctggtgg tgctgggcct ggcagcgttc
     5581 gcaccgaggg ggatcgtcaa gccgcctgag cgcggccagc ttatctacga gaaggagtcg
     5641 gcgtacaact acatccaggt cgtgcaggag ggcaagcgag tcgcccttca gctcaacgag
     5701 ggccacgcca tacactccat ctataacccc gaacaactgc ttacgggcgg gccgtgggac
     5761 tactgggcgg tagcgcctta tttcgcggag gggttcgagc cgtccgacta caagggtatg
     5821 gcgatgatcg gctcggcggc gggtacagcc tcgcacatta ttaccgaggc ttacggcccc
     5881 atcccgatgg acggcgtgga aatcgaccct gagatcgtag ccgtgggaag ccgctatttc
     5941 gccctggaca cccttccgaa cgtcaccagc cacgtcgagg acgggcgcac gtaccttcag
     6001 acgaacggcc gggacaagaa atggaccgta ataggcatcg atgcctaccg ccagccctac
     6061 atcccctttc acttgaccac ggtcgagttc ttccaggaag tgaaggacca cctcacgccg
     6121 gatggctccg tgatgatcaa cgccgggcgc acgctccacg atacccgcct agtggacgcc
     6181 ctggccaaca cgatgttgca ggtgtacccg aacgtgtacg tcatagacgt gcccacgatg
     6241 gcgaacagca tggtgatagg caccgccagc ccctcgcgca ttgagaactt cgccgccaac
     6301 gccgcccgca tcgacaaccc agtgctcaag caaatcttcg atgtgagcct ggagcaaggc
     6361 aacatccgcg aagtcaagcc cgacaccgcc aagctcgtct ttaccgacga caaggccccc
     6421 atcgaagagg ttatcaacca gatcatcctc ggctacgtag gcgaagcggg gaggtagacg
     6481 aaattcgaaa ttcgaaattc caaattattc aggggggtag gcatgaactt gatgctcagg
     6541 cgctcgactc gtaccgcgac ttctgaagag gtattgatct tcgatgccga caaccgggac
     6601 gctaacggcg cggcggtgaa catcggcaag atagacgtgc atttcctcga cgaccaggtg
     6661 gtgggcacgc tgctgctgtg gaacgagttc atcaccggct tcgaccaggc gcacgggccg
     6721 caggacgacg agtcgctcga tgtggtaatc caccagatta tgactgagat caccgacgct
     6781 ttgggcgtgc cgagcgagtt cggcatcgag gtctattacc ccagcgtgca gaactaccag
     6841 ttcgtgagca gctacgccga cgacgaggca gaggacgaga gcgatgtggc agacggcgga
     6901 gcctatggag gaagctcggc ggtcgggctg gacacggagt accttcgcga cgaggacgag
     6961 gagccagatg cgaacgcaga cgatttcgtc aagcgactcc gcgagcggcc gtaaccccgc
     7021 acgtcccgcc ggggtgccgg tccgcgacgg catatacagc gtgcttgccc tgcgtccata
     7081 cactgatctg ctgcatggca tctccacccg cctgtcgccc gacggcaccg actggaacct
     7141 gagcgcccgc cgtggcaccc ccgccaaccc gcccgacccg gaaatagccc tcgccaaccg
     7201 caccggcctc gcgcaccggc tcggcatatc cctcgacagg atggtaggct gccagcaggt
     7261 gcacgggaac gaggtggcgc tggtagggga ggaggacgcg ggcaagggta tgcttcccgg
     7321 ccggccggcc ctcccggact gcgatgcaat gataacggcc acgcccggcc tgtacctgtt
     7381 ggccctgt
//
LOCUS       DASRWZ010000397         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_582394, whole genome shotgun sequence.
ACCESSION   DASRWZ010000397 DASRWZ010000000
VERSION     DASRWZ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582394"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFH60_06130"
     CDS             complement(<1..453)
                     /locus_tag="VFH60_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6313395.1"
                     /translation="MRKQLWADGLLAIVTLIWGSTFVLVKDMVEQVPVMTFLAARFAI
                     GGLVLAVSVLLLGRWRALTMRELGWGVLIGAALWAGYAFQTLGLQGTTASKAGFITGL
                     SVVLVPVLGLFVLRHRPGLWAWMGIALATSGLALLSLRFDERLEVNRGD"
     gene            665..1675
                     /locus_tag="VFH60_06135"
     CDS             665..1675
                     /locus_tag="VFH60_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET6313396.1"
                     /translation="MNKQGSRGQAERYEEEEAEARSRAEAVAVRPVQAELFTISARGE
                     LMQPVADIPALGPTSSLDVARWWFKRHLEQQKRPINTIDSYMYDLALFQSTLGNKTLD
                     RIDKTDLANFLGQANNKSTRKRRLTSLQGLYRYLVNKEKVLEKNLADSFFPDYIPLKT
                     PQVLFPAEQEALLEAARAENARTALIVYLLLKLGLTRTELLALKVDHVDISEPEQPVV
                     YVYYDDIRSQKKERKLGANLEFTEAFQAYVAEFEPVSRMFNLLPQSVNKMVDRVAEEA
                     GISKRVTPQSLRDTFAVEQAKAGADEKKLLLILGLAPDSRNRKSVQRYIKLAAPPVAG
                     VV"
     gene            1677..2135
                     /locus_tag="VFH60_06140"
     CDS             1677..2135
                     /locus_tag="VFH60_06140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET6313397.1"
                     /translation="MPDSLPPGDKSPLVVQHTFRVRYIETDAMGIVHHSNYLAWMEMG
                     RTEFMRHFGFTYRQLELTGVLLPVLELKVRYYTPAYYDDELVISTWVEELSRTRLLLS
                     YRIERPADGKLLTEGTTLHTFIGKDGRPIRITHHKDAWERLQSMVPHLGT"
BASE COUNT          475 a          678 c          660 g          348 t
ORIGIN      
        1 gtcgccccgg ttgacttcca ggcgctcatc gaagcgcagg ctgagcaggg ccaaccctga
       61 cgtggcaaga gcgatgccca tccaggccca cagtccgggg cggtgtctca gcacgaacag
      121 tccgagcacc ggcacgagca ccaccgaaag ccccgtgatg aaccccgctt tactggccgt
      181 cgtaccctgc aggccaagcg tctggaaagc gtacccggcc cagagcgcgg caccaatcag
      241 cacgccccag ccaagctcgc gcatcgtcag ggcgcgccac ctgcccagca gcagcaccga
      301 taccgccagc accagcccgc caatcgcgaa gcgcgccgcg agaaaggtca tcaccggcac
      361 ctgctcgacc atgtccttca cgagcacgaa ggtagacccc cagatgaggg tcacgatagc
      421 cagcagcccg tcggcccata gctgcttgcg caacggcaca acctgattgg cggcagcaga
      481 actcaccaga cagtatagcg gaatttcgaa tttcggattt cgagtttgtt ggatacctac
      541 taaatccgaa attcgcattc caaaatccca attttggtag gggacatatc gcttgcaaac
      601 ggctcgcact tgacatataa tcagggccgt aggcgggcgg attgtggaaa agtaggaggt
      661 ggacatgaac aagcagggtt cgagagggca ggcggaacgg tacgaagagg aagaggccga
      721 ggcgaggagc agggcggagg cggtggcggt gcgcccggtg caggccgagc ttttcacgat
      781 cagcgcgcgg ggcgagttga tgcagccggt ggccgatata cccgcgctgg ggccgacttc
      841 gagcctcgac gtggcgcgct ggtggttcaa gcgtcacctg gagcagcaga agcgccccat
      901 caacactatc gactcttata tgtacgacct ggcgctgttc caatcgacgc tgggcaacaa
      961 gacgctcgac aggatagaca agaccgacct cgccaacttc ctggggcagg caaacaacaa
     1021 atcgacccgc aagaggcgct tgacctcgct gcaggggctt taccgctacc tggtgaacaa
     1081 ggaaaaggtg cttgagaaga acctggcaga cagtttcttc cccgattaca tccccctcaa
     1141 aaccccgcag gtgcttttcc ccgccgagca agaggccctg ctcgaggccg cccgcgcgga
     1201 gaacgcccgc accgcgctga tcgtttacct gctgctgaag ctgggcctga cgcgtaccga
     1261 gctgttggcc ctcaaggtgg accacgtcga tatcagcgag ccggagcagc cggtggtgta
     1321 cgtgtactac gacgatattc gctcgcagaa gaaagagcgc aaactggggg ccaacctgga
     1381 gttcacggag gcgttccagg cttacgtggc cgagttcgag cctgtgtctc ggatgttcaa
     1441 cctgctgccg caatcggtga acaagatggt ggaccgggtg gccgaggagg caggcatcag
     1501 caagcgggtt acgccccaat cgttacgcga tacgttcgcg gtagagcagg ccaaggcggg
     1561 cgccgacgag aagaagctgc tgctgatact gggcctagcg cccgacagcc gcaatcgcaa
     1621 gagcgtgcag cgatacataa agctggcggc accgccggtg gccggggtgg tttagtatgc
     1681 cggatagcct gccgccgggc gacaagtcac cgctggtcgt gcaacacacc ttccgcgtgc
     1741 gctacatcga gaccgacgct atgggcatcg tgcaccacag caactatctg gcctggatgg
     1801 agatggggcg caccgagttc atgcggcact tcggcttcac ctaccgccag ctagagctaa
     1861 ccggggtgct gctgcccgtg ctggagctga aggtgcgcta ctacacgccc gcctattacg
     1921 acgacgagct tgtaatcagc acctgggtgg aggaattgag ccgcaccagg ctgctgctat
     1981 cctaccgcat cgaacgcccc gccgacggca agctcctcac cgaaggcacc acgctgcaca
     2041 ccttcatcgg caaggacggc cgccccatcc gcatcaccca tcacaaggac gcctgggaac
     2101 gtctccaatc tatggtcccg cacctgggta cgtaggccgt cgcggagttc tattcgccac
     2161 g
//
LOCUS       DASRWZ010000398         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_444920, whole genome shotgun sequence.
ACCESSION   DASRWZ010000398 DASRWZ010000000
VERSION     DASRWZ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444920"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..>1604
                     /locus_tag="VFH60_06145"
     CDS             265..>1604
                     /locus_tag="VFH60_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313398.1"
                     /translation="MVRLRRVRVWSLFVVLLLVSVAPGWGVGRGAAQEAVPEFGYVSA
                     MALDASGNGWAWATSPPQTFFNGFLIRIEGGAWRVAASAEQNAGMVPSGAEAAKIVLT
                     GKGDFGWAIGQIRSLEGEEFAPLLMRLRNGTWAPARHSFPTTMRLLDITIAPDESDGW
                     MTAYDEAEERFRLLRLRNGSWNYVSLPAGGGALEAVALSPDGKQGWAAGPRSSTSDPF
                     ATPAAYRLNNGQWQVVPGDFSGAPIAASAVVADNAGNGWLIGRLLVDIGLGSSGTARA
                     ARLSNQEAIVPRDLLIRLSRNAEPRVVDLEMQQPGPVNASDADFSLTGLTVDSAGRGW
                     LAASYYLGTREDPPEFETLFAPGLYRLQGDAATLVPVAEAGYGGEQNFSPLEVASSRE
                     GAHTWIGGSDGYGFGRLEEIQEPWGHERPAAAGPLPGGGRCFAEVPHCLRGVFA"
BASE COUNT          258 a          370 c          666 g          310 t
ORIGIN      
        1 gtggggttcg aggggttggc ccaggtgggt tatgagggag gagcatgagg gtgccttaag
       61 aaaggggcgg caccggggtg ggggacgaga tttctcgcta cgctcgaaat gacaagagag
      121 ggctggtacg tcataagcaa cgatcaagaa tgggaggctg atccttcgct acgctcagga
      181 tgacaatggt actcttgggc ggtgatcgtc tccattcatg cttatgaaat gtcttgtaag
      241 ggcaacgatt aggaggagag gtagatggta cgtctgcgtc gggtgagggt gtggagcttg
      301 tttgtggtgc ttttgctggt gagcgtggcg ccgggttggg gtgtggggcg gggggcggcg
      361 caggaggctg tgcctgagtt tgggtatgtg agcgcgatgg cgttggatgc cagcgggaac
      421 ggctgggcgt gggcgacttc gccgccgcag actttcttca acggctttct gatacggata
      481 gagggcggcg cgtggcgggt ggcggcttcg gcggagcaga acgcgggtat ggtgccgagt
      541 ggggcggagg ctgccaagat cgtgctgacg gggaagggcg actttgggtg ggcgattggg
      601 cagattcgga gtttggaggg tgaggagttc gcgccgctgt tgatgaggct gcgcaacggc
      661 acctgggcac cggcccgaca tagttttccg acgaccatgc gcttgctgga catcacgata
      721 gcgcccgacg agagcgacgg ctggatgacg gcgtacgatg aggccgaaga gcgtttcagg
      781 ctgctgagat tgcgcaacgg cagttggaac tacgtatcgc tgcctgccgg tgggggtgct
      841 ctggaggctg tggctctcag tccggacggc aagcaggggt gggcggcagg gccgcgaagc
      901 agcacctcgg acccgtttgc tacgcctgcc gcgtacaggc tgaataacgg gcaatggcag
      961 gtggtgccgg gggatttttc gggcgcgccg attgcagctt cggcagttgt ggcggacaac
     1021 gcggggaacg gctggctgat cgggcggctg ctggtcgata ttgggctggg aagctcaggg
     1081 acggcgcgtg cggcgaggct ctccaaccag gaggcgattg tgccgcgcga cctgttgata
     1141 cgcttgtcgc gcaatgccga gccgagggtg gtagacctgg agatgcagca gcctgggccg
     1201 gtgaacgcct ccgatgcgga cttcagcctg acggggttga cggtggatag cgcggggcgg
     1261 gggtggctgg cagcttcgta ttatcttggg acgcgtgagg acccgccgga gtttgaaacg
     1321 ctgttcgcgc ccggccttta ccgcttgcag ggggatgcgg cgacgctggt gcctgtagcc
     1381 gaggcggggt acggaggcga gcagaacttc agcccgctgg aggtggcttc ttcacgggag
     1441 ggggcgcata cctggattgg agggtcggac gggtacgggt ttgggcggct ggaggagata
     1501 caggagccgt gggggcatga gaggcccgcg gcggccgggc cgcttcctgg tggcgggcgg
     1561 tgcttcgcgg aggtgccgca ttgtctgcgg ggcgtgttcg cgca
//
LOCUS       DASRWZ010000399        13872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_445029, whole genome shotgun sequence.
ACCESSION   DASRWZ010000399 DASRWZ010000000
VERSION     DASRWZ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 13872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13872
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445029"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VFH60_06150"
     CDS             <1..751
                     /locus_tag="VFH60_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313399.1"
                     /translation="AIHYVYSTYLPGRTPHIYASVTKGGSSWPDSTMKNAGAYFDSVD
                     IHNYNSDTTSYTRQVHGWMNANGKANAPLWLSEWATYRGGYQNAGTGVKTIINNLIRG
                     SRPGNDYIYGSHLFTFYDWDGFAGGFQNFQGLVGPTGTKLASFYALRMGTRALNGCKA
                     TYQSTANNSNLLAITTKDAAGSVYLLVTNSAANTSYTVDANLSALLSSGTGTLYQFDS
                     THNDVVNGSPVLSNGHVTFPIPGTSAILLKY"
     gene            983..2725
                     /locus_tag="VFH60_06155"
     CDS             983..2725
                     /locus_tag="VFH60_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313400.1"
                     /translation="MAHNPGLYRTLLSRYLAPQRLRVVLLAVLLLGSIGLQLLNPQIL
                     RRFIDTVTGSGPQGGVAGIAALFLGLAIFQQVLSVGATWASERVAWSATNSLRADLAL
                     HCLRLDLSFHKARTPGELIERIDGDVTALANFFSQFVVQILGSVLLLLGVLAVLWTVD
                     WRAGGALTVFAVVTLGAMLGLRTIATRFWKAYRQASAELFGFVEEHLAGTVDIRANGA
                     QPYVMHRLYRFMRARFQTGRRARLASSIPWSIPSLAEALGIGIAFALAVSLYGAGSIS
                     LGTAFVVYFYTQALFQPMHMLSNQLEDFQKASAGAIRVQELMSTHSALTDGSLTEFAP
                     GAPAIEFRDVTFSYEGDDTVLRDLSFRVAPGEVLGLLGRTGSGKTTVTRLLLRLYDPT
                     AGSITLGGVELRAAELAALRRQIGMVTQDVQLFRGSIRDNLTFFDDSVPEARIWEALE
                     SLGLVEWCRALPQGLDTIIEGGGGLSAGEAQLLAFTRVFLKDPGLVILDEASSRLDPV
                     TERLVERAVVGLLRGRTGIIIAHRLSTVDRADTILILERGAIAEHGPRPALVSDPASR
                     FSQLLRTGVEEVLV"
     gene            2722..4587
                     /locus_tag="VFH60_06160"
     CDS             2722..4587
                     /locus_tag="VFH60_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313401.1"
                     /translation="MTGATEASASGTPRMTRLQTLRFLWRLIWFRGDLFWSNCFSIVM
                     LFVTGLVPGFVAQQFFNQLAEGGTPDLLWLTALLVMSALGRITFLVGCQLTNAPFMLS
                     AESLMQKNMFRRILSLPAARALPDSPGEAVSRFRDDTSGVTEAMITLNDFIASTVFAV
                     IAFTIMARINLMITIGVFLPLSVVGLIANLAMRRIEIYRKANREATGKVTGFLGEVFG
                     AVQAVQVAGADESVNAQFRKLNNTRLHRAVRDRVFDQLLHSIFWNAVNFGLGFILLFS
                     AQAMQDGSFTVGDFALFAYFLGWITDFTSLFGVIVARYKQAGVSFARMIRLLGGARPE
                     TLVESGPVYLSGPYPDLPGVPPHAAGKLDTLEVVGLTYLHPDSTRGVRELSFRLERGS
                     FTVITGRIGSGKTTLVQVLLGLLPPSAGTIRWNGEPVSNPASFFVPPQTAYTPQVPRL
                     FSDTLSNNILMGLPEEGVDLAQALHLAVMEPDIAGMPDGLDSLVGSRGVRLSGGQVQR
                     TAAARMFVRGPDLLVFDDLSSALDVETEATLWERVFARPDATVLAVSHRRAALRRADN
                     IIVLKDGALEAMGTLPELLATSREMQHLWHGEEAAETEAGKIEEPGPERVLGVLT"
     gene            complement(4719..5897)
                     /locus_tag="VFH60_06165"
     CDS             complement(4719..5897)
                     /locus_tag="VFH60_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6313402.1"
                     /translation="MSDTPTPYGQSDNSIEDARLRHWRLILGGGDAEGTGVSLAGADL
                     GIDKALSALYDPERAGGLGGSSPNVARWLGDIRNYFPSSVVRVMQQDALERLDLKRML
                     LEPEMLAAVEPDVHLVATLLSLKSVMPNKTKDTARLVVRRVVEDLEKRLANPLRQAIL
                     GSLHRATRNRRPRHNEIDWHRTIRANLKHYQPEYRTIIPHTRIGYGRKKMQLRDIILC
                     VDQSGSMATSVVYSGILGAVLASLPSVRTHMVVFDTSVVDLTEDLHDPVELLFGTQLG
                     GGTDINRALTYCQGLVRQPHDTIVVLVSDLYEGGNREEMLKRVAWLVGSGATLVALLA
                     LGDDGAPSYDHSVAAAFANLGVPAFACTPDLFPELMAAAISRQDIGQWLALHDIARKG
                     "
     gene            complement(5894..8233)
                     /locus_tag="VFH60_06170"
     CDS             complement(5894..8233)
                     /locus_tag="VFH60_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HET6313403.1"
                     /translation="MSNSVHIYGIRHHGPGSARSLREALAELRPDCILVEGPPDAEGV
                     LPLLAQPEMRPPVALLVYLPDQPRRAVFYPFAVFSPEWQAIAYGLGEGVPVRFMDLPQ
                     AHSFAEPLPEVVLQPEQATPPVEGILEGKGEEENGDDETASLAFNPREDPLGWLARAA
                     GHSDGERWWESMVEQRQGSEDLFAAILEAMTALRAALPDLDEPEEARREQLREAHMRK
                     TIREAQEEGFSNIAVVCGAWHAPALAGTLKDLPAEKDDTALLKTLQKVEVKATWVPWT
                     YGRLSTSSGYGAGIHSPGWYHHLWTTREHVVVHWMANVAKLLRDQDLDASPAHIIEAV
                     RMAEALASLRERPAVGLTELNDATRAVLLFGDDTPMGLIHSKLIVGETLGNVPTETPT
                     VPLQVDLQKQQKRLRLPVEVTPRTLDLDLRGETDLQRSHLLHRLSLLGVPWGQLERVA
                     GGKKGTFHELWALQWQPELAVSLIEAGMWGNTIADAATGYIQHAAARTSTLPGLTRLL
                     DIALLADLGDAIGGIMSRVEETATLSSDVTHLMEALPPLANVLRYGNVRRMDTGSVAH
                     VVSGIVARVCIGLPGAVASLNDEAASEMFTHILATNDAIALLQSDEHSEAWRATLQQL
                     VNQRGVHGLIAGRACRILLDAAYIDQEEAARQMSLALSTSVEPAQSAAWIEGFLKGSG
                     LVLLHDKALMATVDNWVAALRPEAFIQLLPLLRRTFSTFSAPERRQIGEWVRRGPVAF
                     PGVEAQENTFDPDLAEAALPLLAQLLGLELPANTGELQR"
     gene            complement(8233..9222)
                     /locus_tag="VFH60_06175"
     CDS             complement(8233..9222)
                     /locus_tag="VFH60_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313404.1"
                     /translation="MKKWSLAGGLLFLGVAVIALISVMWLTGISIEWPNGASRTLANS
                     SPSEAAYVDMAISPDWQTLALGTLQDGAQVWSVKDNKMLYSTEPGRVQTVAISPDGQM
                     LALFWKSKDESSHRIELRQLSDGKLLRTLGDQEEWVYSLTFSADGQSLAVAASGEVQL
                     FGVSDGKELRTIHATAWEVALSPDGKLVAAGGEPVGVWRVADGAPLHTVKGWHYMLFS
                     PDGKVLATVDSVLDSNVNLWDTSTGTLIGTLEAPAPGMEQIHSLAFSPDSETLAAGTN
                     GYSVHVWQVSTGKIQRTIRLGTNNPGEDVVFSPDGRTLAVQTNYKVQFWDLEK"
     gene            complement(9256..10335)
                     /locus_tag="VFH60_06180"
     CDS             complement(9256..10335)
                     /locus_tag="VFH60_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6313405.1"
                     /translation="MTTLLREHAEQQYAGELEALSKVDDRPRPPNWRLSPWAVATYLM
                     GGRLSNGFEVSPKYIGNRRIIEIAIATLTTDRALLLLGVPGTAKTWVSEHLAAAISGN
                     STMLVQGTAGTAEEAIRYGWNYARLLAEGPSERALVPSPVMRAMQLGTIARVEELTRI
                     PSDVQDALITILSEKTLPIPELEGEAQAVKGFNVIATANNRDRGVNELSSALKRRFNT
                     VVLPVPETLEEEVQIVRQRVESLGRALELPVEPPALEEVRRVVTVFRELRDGVTLDKK
                     TKLKQPSGTLSTAEAISVVNSGLALAGHFGDGRLHAADMASGMVGAVVKDPVQDRVVW
                     LEYLETVVKERPGWKDLYRACREQM"
     gene            complement(10335..11915)
                     /locus_tag="VFH60_06185"
     CDS             complement(10335..11915)
                     /locus_tag="VFH60_06185"
                     /inference="COORDINATES: protein motif:HMM:NF039502.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HET6313406.1"
                     /translation="MQSNSQITEAWDSLVAVALLGTERRTASLPSLGGTLGALLSQLS
                     EGVKAEQAFLGASTLLAAYTRAGRQPVMSRSPEPSPCEPEEHPYCTHRAAGHLALMLG
                     GQHDEVLPEWLQALERRGRLVPPEHVSALLDLGASQSHLRDAIRLVAGKRGRWLARQN
                     PQWEYLAPLEADRDSWEAKWETANFGARRAMLEQVRRVDPARARELITTSWKEDKASE
                     RAAFIATLRTGLSPEDEPLLEAALDDRSKDVREEAAELLAQLPGSGLVSRAIEAVLPF
                     ISFKELRQRRLFGGTNDKSYIEVALPQDYTPQMKRDAVQQKSPYTRLGERAYWLSQLL
                     GRIPPGYWSERWRQPPGAIVAATANTKEWRDMLMGAWTDATRRHKEAVWARALLAHSQ
                     DIVVEGAVQNLLSVLPASEREQFVLGALKRNPAALYTAAVPVSLLWQCESQWSTELSR
                     TVLRSVSHYIVTTSAYGGWEVRQSLQGIARSVSPSILEEATAVLSERAAANPFFAGAV
                     DEFLATVQFRRDMLKELD"
     gene            complement(11939..12808)
                     /locus_tag="VFH60_06190"
     CDS             complement(11939..12808)
                     /locus_tag="VFH60_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313407.1"
                     /translation="MDDLVRNGLASAQTQPFTYWERTAARLVDAQAPGAARLVKEMAA
                     ITQSGDGWAERMLAAVGRLYLLLEGYRRIDSLPPQTQADIRTLLGWTLNQEELLAEAG
                     VRDRWVVLGQRVEDEDRLKVKRTLLLGEESGQWALVLHFAHGKDPLDVSLAPGTIIEA
                     TLVFYEGSGPKRALVKDKHGPPEPLGAMPGKELLAAIEAYSRAQTSNPWLERFPFSLA
                     EVIPVRQDAGNSRSGWAVRDAQGYTLPLASHPDGWWPLLALSGGRPISIFGEWNGAYL
                     LPLSAWSGTFVQF"
     gene            complement(13058..13315)
                     /locus_tag="VFH60_06195"
                     /pseudo
     CDS             complement(13058..13315)
                     /locus_tag="VFH60_06195"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
BASE COUNT         2492 a         4449 c         4221 g         2710 t
ORIGIN      
        1 cgccatccac tacgtataca gcacctactt gcctgggcgc accccgcaca tctatgcttc
       61 ggttaccaag ggcggaagct cctggccgga cagcacgatg aagaacgcgg gcgcctattt
      121 cgacagcgtc gatatccaca actacaactc cgacaccacg agctacaccc ggcaggtgca
      181 cggctggatg aacgcgaacg gcaaggccaa cgcgccactc tggctgagcg aatgggcgac
      241 gtatcgcggt ggctaccaga acgcagggac gggggtgaag acgatcatca acaacctgat
      301 ccgaggctcg cgtcccggca acgactacat ttacggcagc cacctgttca cgttctacga
      361 ctgggatggc ttcgcgggcg gcttccagaa cttccagggc ctggtagggc caacgggcac
      421 caagcttgcc agcttctacg cgctacgcat gggcacgagg gccttgaacg gctgcaaggc
      481 tacgtaccag agcacggcca ataacagcaa cctgctggct atcaccacca aggacgcagc
      541 cggtagtgtc tacttgctgg taacgaacag cgcggcgaac acgtcttaca cggtggacgc
      601 caatctctcg gcgctgctga gtagcggcac cggcacgctg taccagttcg actccacgca
      661 caacgacgtg gtcaacggaa gccccgtgct ctcgaacggc cacgttacct tccctattcc
      721 gggtacgtcg gccatcctgc tcaagtacta agcgccgcct gccacacgaa ttgggagccg
      781 caaggcggag gggccaccca cctgggtggc ccctccgccc tgtcacttga aagctcggtc
      841 tgcacgccga ggcagattac atggttggtc ccgccggata gcaagctgtt gactgtataa
      901 tcgtgggacg ggcggcgcgc ttagctgcca acttctgtct gccgagggta cccgcataga
      961 cgcacctgcc tcacctggct ctatggcaca caatcccggc ctataccgca cgctgctatc
     1021 caggtacctg gccccgcaac ggctgcgggt ggtactgctg gccgtgctgt tgctgggcag
     1081 catcggcttg cagttgctca acccccagat cctgcgccgc ttcatcgata cggtgaccgg
     1141 ctccgggccg cagggaggcg ttgccggcat cgcggcgctc ttcctgggcc ttgccatctt
     1201 ccaacaggtg ctcagcgtcg gcgcgacctg ggcgagcgag agggtagcct ggtccgccac
     1261 taactcgttg cgcgcggacc tcgccctcca ctgcttgagg ctcgacctga gcttccacaa
     1321 ggcgcgcacc ccaggcgagc tgatagagcg catcgacggt gacgtgaccg cgctcgctaa
     1381 cttcttctcg cagttcgtgg tgcagatact tggcagcgtt ttgctcctgc tgggcgtgct
     1441 cgccgtgcta tggacggttg actggcgcgc tggaggggcg ctcaccgtgt tcgcggtggt
     1501 gactctgggg gctatgctgg ggctgcggac gattgccacc cggttttgga aggcctacag
     1561 gcaggccagc gccgaattgt tcggcttcgt tgaagagcac ctggcaggca cggtggacat
     1621 acgagccaac ggcgctcagc cctacgtgat gcaccgcctg taccggttca tgcgagcgcg
     1681 cttccagacc gggcgacgcg cgaggctggc gagcagcatc ccgtggagca tacccagcct
     1741 tgccgaggcc ctggggattg gcatagcttt cgccctggcc gtgtcgctgt acggggccgg
     1801 gagcatcagc ctgggcacgg cgttcgtggt ctatttctac acccaggccc tcttccagcc
     1861 catgcacatg ctctccaacc aactggaaga cttccagaag gcgagcgccg gggccatccg
     1921 ggtgcaggag ctaatgagca cgcatagcgc cctcaccgat ggcagcctga ccgagttcgc
     1981 gcccggcgcg cccgccatcg agttccgcga cgtgaccttc tcctacgaag gcgacgatac
     2041 cgtgctgcgc gacctgtcgt ttcgggttgc gccgggcgaa gtgctcggcc tgttggggcg
     2101 taccggctcg ggcaagacca cggtcacgcg cctgctgctt cgcctctacg acccgacggc
     2161 ggggagcatc acgctgggcg gggtggagtt gcgcgctgcg gaactggccg ctctacggcg
     2221 gcaaatcggc atggtgaccc aggacgtgca gcttttccgg ggcagcatcc gcgacaacct
     2281 gaccttcttc gacgatagcg taccggaggc gcgcatctgg gaagctttgg agtcgttggg
     2341 gctggtggag tggtgccgcg cgctgccgca gggactggat accatcatcg agggaggtgg
     2401 cgggctgtcg gcgggtgagg cgcagctcct ggcctttacc cgcgtcttcc tcaaagaccc
     2461 cggcctcgtc attctggacg aggcttcgtc gcggctcgac cccgtaaccg agcggctggt
     2521 agagcgggca gtagttggcc tgctgcgcgg gcgcaccggc atcatcatcg cccaccgcct
     2581 ctcaaccgtg gaccgtgccg atacgatctt gatcctggaa cggggagcaa tcgcggagca
     2641 cggcccgcgc cccgcactcg tctccgaccc ggcatcccgc ttctcgcaac tgctgcgcac
     2701 aggggtcgag gaggtgctgg tatgacaggc gcgaccgagg catccgcgtc cggcacgccc
     2761 cgcatgacga ggctgcaaac cctgcgcttt ctatggcggc tgatctggtt tcgtggcgac
     2821 ctcttctgga gcaactgctt ctctatcgta atgctcttcg tcacggggct ggtgccgggc
     2881 tttgtcgcgc aacagttctt caaccagcta gcggaaggcg gcactccgga cctcttatgg
     2941 ctgactgcgc tgctggtgat gtcggccctg gggcgcatca ccttcctggt aggctgccag
     3001 ctaaccaacg ctcctttcat gctcagcgcc gagtcgttga tgcagaagaa catgttccgg
     3061 cgtatcctca gcctgccggc ggcgcgcgcc ctacccgatt cgcccggaga ggcggtgagc
     3121 cgcttccgcg acgacacgtc gggggtgacc gaagccatga ttaccctcaa cgacttcatc
     3181 gcctccaccg tctttgccgt gattgccttc acgatcatgg cgcgcatcaa cctgatgatt
     3241 acgataggcg tcttcctgcc gctctcggtg gtgggcctca tcgcgaacct ggctatgagg
     3301 cgcatcgaga tctaccggaa ggccaaccgc gaggcgaccg gcaaggtgac cgggttcctg
     3361 ggggaggtct tcggggcggt gcaggcggtg caggtggccg gggcggacga atcggtcaac
     3421 gcgcagtttc gcaagctcaa caacacacgc ttgcacaggg ccgtgcgcga ccgggtgttc
     3481 gaccagctgc tccactcgat cttttggaac gccgtcaact tcgggcttgg ctttatcctc
     3541 ttgttctccg cgcaagcgat gcaagacggc agcttcacgg tcggtgattt cgcgcttttc
     3601 gcgtacttcc tgggctggat aaccgatttt acgtcgctgt tcggtgtaat cgtggcgcgc
     3661 tacaagcagg ccggcgtgtc gttcgctcga atgatacggc tgctgggagg cgcgcggcct
     3721 gagaccctcg tggagtcggg acctgtctac ctgagcggcc cctaccccga tctgccgggc
     3781 gtaccgcccc acgccgccgg gaagctcgac accctggaag tggtgggtct tacgtacctg
     3841 cacccggaca gcacgcgggg cgtgcgcgag cttagcttcc gcctggagcg ggggtcgttc
     3901 acggtgatta cgggacgcat aggctccggc aagaccacgc tggtgcaagt gctgctcggc
     3961 ctgctgccgc ccagcgcggg cacgatacgc tggaacggcg agcctgtgtc caatcccgcg
     4021 agcttctttg tgccgcctca gaccgcatac acgccgcagg tgccccggtt gttcagcgac
     4081 acgctcagca acaacatcct tatgggcctg ccggaggagg gggtggacct cgcccaggcg
     4141 ctacacctcg ccgtcatgga gccggacatc gcagggatgc ccgatgggct ggatagcttg
     4201 gttggctcgc gcggcgtgag gctgtccggc gggcaggtgc agcgcacggc agccgcgcgc
     4261 atgttcgtgc gggggccgga cctgctggta ttcgacgacc tctccagcgc gcttgacgtt
     4321 gagaccgagg ccacactgtg ggagcgtgtc ttcgcccggc cggacgccac ggtgcttgcc
     4381 gtctcgcacc gccgggccgc cctgcgccgc gcggacaaca ttatcgtgct gaaggacggc
     4441 gcgttggagg ctatgggcac tctgccggaa ctgcttgcca cctccaggga gatgcagcac
     4501 ctctggcatg gtgaggaagc ggccgagacg gaagccggga agatcgaaga gccaggccct
     4561 gagcgcgtcc tgggggtcct cacataaggc gcagccgcat catgtttgtc ccgcgatacg
     4621 tcacgctgac aaggggtacg actccagggc caacagaaaa gccctccgca agcgaggcat
     4681 cataccgaga actgccaggt aatacaggtc tgagatgctc agccctttct ggcaatatca
     4741 tgaagggcca gccactgtcc gatgtcttgc ctgctgatag ccgcggccat tagttcgggg
     4801 aagaggtccg gcgtgcaggc gaaggcaggt acccccaggt tcgcgaatgc ggcggccacg
     4861 ctgtggtcgt agctgggcgc gccgtcgtca ccgagggcca gcagggctac cagggtcgcg
     4921 ccggaaccta ccagccaggc gaccctcttt agcatctcct cgcggttgcc gccttcgtac
     4981 aagtcactga cgagcacgac tattgtgtcg tgcggctggc gcaccaagcc ctggcagtag
     5041 gtgagcgccc ggtttatgtc ggtgccccca cccagttgcg tgccgaaaag cagctccacg
     5101 gggtcgtgca ggtcctcggt caggtccact accgaggtat cgaacaccac catgtgagtg
     5161 cgcacggatg ggagggaagc cagcaccgcg cccagaatcc cagagtaaac cacggatgta
     5221 gccatagagc cactctggtc cacgcacagg atgatgtcgc gtagctgcat cttcttgcgc
     5281 ccgtacccga tgcgggtgtg aggtattatc gtgcggtact ccggctggta gtgcttgaga
     5341 ttggcccgta ttgtgcggtg ccagtctatc tcattgtgcc tgggacggcg gttgcgtgtc
     5401 gctcggtgga ggctgcccag gatggcctgg cgcaaggggt ttgccagccg cttctccagg
     5461 tcctcaacta cacggcggac caccaatcga gccgtgtcct tggtcttgtt gggcataacg
     5521 ctcttcaggg agaggagcgt ggcgacaaga tgcacgtcag gctcgaccgc tgccagcatc
     5581 tccggctcca gcagcatccg tttgaggtcc aacctctcca gggcgtcttg ctgcatgacc
     5641 cgcactacgg aagacgggaa gtagttgcgt atgtcaccga gccagcgcgc cacgttgggt
     5701 gaggagccgc cgaggccccc cgcccgctcg gggtcgtaca acgccgacaa cgctttgtct
     5761 atgcccaggt ctgcccctgc cagggatacc cccgtaccct cggcatcccc gccgcccagt
     5821 atgagccgcc agtgccggag ccgtgcgtct tcaatggagt tgtcggactg cccgtaaggt
     5881 gtgggtgtat cgctcacctt tgtaactccc ccgtattggc cggtagttcg agtcccaaga
     5941 gctgcgccag gagcggtaac gcggcctcag ccaggtcggg gtcgaatgta ttttcctggg
     6001 cctcaacgcc tgggaaggcc acgggtcccc tccgcaccca ttcaccaatc tggcggcgct
     6061 ccggtgcgga aaacgtggaa aaggtgcggc gcaggagagg caggagctgg atgaatgcct
     6121 ccggccgcag ggctgctacc cagttgtcta ccgtagccat cagcgccttg tcgtgcagca
     6181 gcaccaggcc gctccctttc aaaaatccct ctatccaggc cgccgactgc gctggttcga
     6241 cggaagtaga gagcgccagg ctcatctgtc gcgccgcctc ttcctggtcg atatatgccg
     6301 cgtccagcag aatgcgacag gcccttccgg caatgaggcc atgcacgccc cgttggttca
     6361 ccagttgttg gagcgtagca cgccaggcct cgctgtgctc atcgctctgt agcaatgcaa
     6421 tagcatcgtt ggtcgccagt atgtgggtga acatttcgga ggccgcttca tcgttgagtg
     6481 aggctaccgc accgggaagc cctatgcata ctctcgctac gataccgctc actacgtggg
     6541 ccacactgcc cgtatccatg cggcgaacgt tcccgtacct gagcacgttt gccagcgggg
     6601 gcagcgcctc catcaggtgg gtcacgtcgc ttgagagggt cgcggtctcc tctacgcggg
     6661 acataatgcc gcctattgcg tccccaaggt ctgcaagcaa agctatatcc agcagcctgg
     6721 tgagtcccgg cagagtgctt gtccgggcgg ctgcgtgctg gatgtagccg gtggcagcat
     6781 ccgcaatcgt gttgccccac atgcctgctt cgataaggct gaccgcaagc tcaggctgcc
     6841 actggagcgc ccacagctcg tggaaggtgc ccttcttgcc ccccgcaact cgctccaact
     6901 gcccccacgg cacgccgagc aggcttagcc tgtgcagcaa gtggctcctt tgcaggtcgg
     6961 tctcgccacg caggtccagg tccagcgtgc ggggggtgac ctcgaccggc agccgcagac
     7021 gcttttgttg cttttgcagg tccacttgca agggtacggt aggggtctcg gtgggtacgt
     7081 tgccgagcgt ctcgcccaca atgagcttgc tgtggatgag gcccataggc gtgtcgtcgc
     7141 caaagaggag cacggcgcgg gtcgcatcgt tcagttcggt aagcccgacc gccggacgct
     7201 cccgcaacga ggccagcgcc tcggccatgc ggacggcttc gataatatgg gcgggcgatg
     7261 cgtccaggtc ctggtcccgc agcaatttag ccacgttagc catccagtgt acgacgacgt
     7321 gctccctggt ggtccagagg tgatggtacc agcctgggga atggatgccc gcgccgtagc
     7381 cgctggaggt agagagccgc ccgtacgtcc agggtaccca ggtggccttt acttccacct
     7441 tttgcaacgt cttgagcaga gcggtatcgt ctttctctgc aggcaagtcc ttcaacgttc
     7501 ccgccagggc cggtgcgtgc cacgccccac agacgacggc tatgttactg aagccctcct
     7561 cttgagcctc gcgtatggtc ttacgcatgt gggcctcgcg caactgctcg cggcgcgctt
     7621 cctccggctc atcgaggtcg ggaagggcgg cacgtagggc ggtcatcgcc tcaaggatgg
     7681 ccgcgaacag atcttcactg ccctgtcgct gctcgaccat gctctcccac cagcgctccc
     7741 cgtcgctatg tcccgcggcc ctggccaacc aacctaaagg gtcctcgcgt gggttgaagg
     7801 cgagggacgc cgtctcgtca tcaccatttt cttcctcccc cttaccttcc aaaatcccct
     7861 ccacgggggg cgtagcctgc tcgggctgta gtacaacctc aggaagaggt tcagcaaaac
     7921 tatgtgcctg gggcaggtcc atgaaccgta ccggcacacc ctcacccagc ccgtaagcaa
     7981 tagcctgcca ctctggagag aagacggcga atggatagaa gacggcgcgc cgtggctggt
     8041 ccggcaggta gaccagcagg gccacagggg gacgcatctc cggctgggcg aggagcggca
     8101 acaccccctc ggcgtcgggc ggcccttcga ccagtatgca atcgggccgc agctccgcta
     8161 gcgcctcgcg caagctacgc gccgagccgg ggccgtggtg gcggatgccg tagatatgta
     8221 ctgaattgct cactacttct cgaggtccca aaactgcacc ttatagttcg tctgtacggc
     8281 cagggtgcgg ccgtccggtg agaagactac gtcttcaccg ggattgttag tgccaagacg
     8341 gatagtgcgc tgaatcttgc cggtgctcac ctgccacaca tgcacgctat agccgttcgt
     8401 gccggctgcc aacgtctcac tgtccggcga gaaagccagg ctatggatct gctccatacc
     8461 gggagccggt gcttccagcg tgcctataag cgtgcctgtg ctcgtatccc acaagttgac
     8521 gtttgaatca aggaccgagt ctacagttgc cagcactttg ccgtcaggag agaacagcat
     8581 gtagtgccag cccttcaccg tatgaagcgg tgctccgtcc gctactctcc agacccctac
     8641 cggctcgccc ccggcagcga ccagcttccc gtctggagaa agggccactt cccaggcggt
     8701 cgcatggatg gtgcgtagct ccttgccgtc gcttacaccg aataactgca cttcaccgct
     8761 ggccgctacc gccaggcttt gcccatcggc tgagaacgtg aggctgtaga cccactcctc
     8821 ctggtcgccc agcgtgcgta atagcttgcc gtccgacaac tgcctcaatt ctatcctgtg
     8881 actgctctcg tctttcgact tccagaagag ggcgagcatc tgaccgtcgg gcgatattgc
     8941 taccgtttgc acccgccccg gctctgtgct gtaaagcatc ttgttatcct tgacgctcca
     9001 gacctgggcc ccatcctgca acgttcccag ggcgagtgtt tgccagtccg gtgaaatagc
     9061 catatctaca taggccgcct cactggggct ggagtttgcc agggtacggg aggccccgtt
     9121 cggccactcg attgatatgc cggtcagcca catgacggag ataagcgcga ttaccgccac
     9181 ccccaggaac agcagccctc cggcgagaga ccatttcttc atggtagtcc taccaggcat
     9241 aatgccgata ctgctctaca tctgctctct gcatgcccgg taaaggtctt tccagcccgg
     9301 acgctccttg acgaccgttt cgaggtactc aagccacacc actctatcct gcacggggtc
     9361 tttgaccaca gcgcccacca tgcccgaggc catgtcggcg gcgtggagtc tgccgtcccc
     9421 gaaatgcccc gccagggcca ggccgctgtt gacgacactt atagcctccg cggtgctgag
     9481 ggtgccgctc ggctgcttga gcttggtctt cttatccagc gtcacgccat cccgcaactc
     9541 gcggaagacg gttaccacac gacgtacttc ttccagggcg ggcggctcta cgggtagctc
     9601 cagggcgcgg cccaggctct ccacccgctg gcgcactatc tgcacctcct cctccaacgt
     9661 ctccggcacc ggcagcacca cggtgttgaa gcggcgtttg agcgcgctcg atagctcgtt
     9721 cacgccccgg tcgcggttgt tggccgtggc gatgacgttg aatcccttca cggcttgcgc
     9781 ctcgccttcc agctcaggta tgggcaaggt cttctcggag aggatggtaa tcagggcatc
     9841 ctgaacgtcg gacgggatgc gggtaagctc ctccacccgt gcgattgtac ccagttgcat
     9901 ggccctcatc accgggctgg gtacaagcgc ccgctcggaa gggccttccg cgagcaggcg
     9961 ggcgtaattc cagccgtagc ggatggcttc ttcggcggtg cccgcggtgc cttgcacgag
    10021 catggttgag ttgccggaga tagcagccgc caggtgctcg gatacccagg tcttggcggt
    10081 gccgggcacc cccagcagga gtagcgcacg gtcggtggtg agcgtagcta tggctatctc
    10141 tatgatccgg cggttgccta tatatttggg tgacacttcg aagccgttag agagacgccc
    10201 acccatgagg taggtagcga ccgcccacgg cgacagccgc cagttgggcg gcctggggcg
    10261 gtcgtctacc ttgctcaacg cctcaagctc cccggcatat tgctgctcgg catgttcccg
    10321 cagaagtgtc gtcatcagtc aagctccttt agcatatcgc gcctgaattg cacagtcgcg
    10381 agaaattcgt ccaccgcacc ggcaaagaat gggttggccg ccgccctctc cgataagacg
    10441 gccgtcgctt cctccaggat ggagggcgac acggagcgag caataccctg taatgattgg
    10501 cgtacttccc agccgccata cgcgctggtc gtcacgatgt agtgactcac actcctgagc
    10561 accgtgcgcg acagttccgt gctccactgg ctctcacact gccatagcag gctcaccggc
    10621 acagccgccg tataaagcgc cgcggggtta cgtttcaagg cccccagcac gaactgttcg
    10681 cgctccgacg cgggcagcac cgacaacagg ttttgaacgg cgccctctac cacaatatcc
    10741 tggctgtgag cgagcaaggc ccgcgcccag acagcctcct tgtgccgccg tgtcgcgtcg
    10801 gtccaggcac ccatcaacat gtcgcgccat tctttggtgt tcgccgttgc cgctactatc
    10861 gcacccggcg gctgccgcca cctttcactc caatagccgg ggggtattct cccaagcagt
    10921 tggctcaacc agtatgcccg ctctcccagg cgggtataag gcgatttttg ttgcacggcg
    10981 tcgcgcttca tctgcggggt gtagtcttga ggtagcgcaa cctcgatgta gctcttgtcg
    11041 ttagtgccgc cgaacagccg tcgctggcgc agttccttga acgagataaa gggtaacaca
    11101 gcctctatag cgcggcttac aagcccggaa cccggtagtt gagccagcaa ctccgccgct
    11161 tcctcgcgca catctttgct gcggtcgtca agcgccgcct cgagcaaagg ctcgtcctcg
    11221 gggctgagac ctgtccgcaa cgtcgcgatg aaagcagccc gctccgaggc cttgtcctct
    11281 ttccaactgg tagttattag ctcgcgcgcg cgggcggggt caacgcgcct cacctgttcc
    11341 agcatagcac ggcgtgcccc aaagttcgcc gtctcccact tagcttccca gctatcacgg
    11401 tccgcttcca ggggagcaag gtactcccat tgggggttct gcctggcgag ccaccggccg
    11461 cgcttacccg cgactaaccg gatagcatcg cgaaggtgtg actggctagc acccaggtcc
    11521 agcaaagcag atacatgctc gggtggcacg agcctgccac gcctttcgag ggcttgcagc
    11581 cattcaggca agacctcgtc gtgctgaccg cccagcatga gagccaggtg tcccgccgcg
    11641 cgatgagtgc agtatgggtg ctcttccggc tcgcacggcg acggctcagg cgacctgctc
    11701 atcacaggct gccggcccgc acgtgtgtac gccgccagga gggtagacgc cccgaggaat
    11761 gcttgctctg ctttgactcc ttcgctcaat tgtgacagca aggcaccgag agtgccgccc
    11821 aggctgggta gggaggctgt gcgacgctct gtgccgagca gcgctactgc tacgagggaa
    11881 tcccaggctt cggttatctg gctgttgctt tgcatatcgg ttccgcgttc actttcacct
    11941 aaaactgcac gaacgtgccg gaccaggcag agagtggcag cagataggca ccgttccatt
    12001 ctccaaagat ggagatcggt ctgccgccgc tcagagccaa taaaggccac cacccgtcgg
    12061 ggtgcgacgc gagcggcaag gtatacccct gcgcgtctcg taccgcccag ccactccggc
    12121 tattcccggc atcttgccgc accgggatta cctcggccag gctgaatgga aaccgctcaa
    12181 gccaggggtt ggaggtctgg gcgcgtgaat aagcctcgat ggcggcgagc aactccttgc
    12241 ccggcattgc gccaagaggc tccggcggac cgtgcttgtc tttcaccagc gcgcgcttcg
    12301 gtcccgaacc ttcgtagaaa acgagagtgg cctctatgat ggtgcccggc gcgagcgata
    12361 catccagggg gtccttgccg tgcgcgaaat gcagcaccag cgcccactgc ccgctctcct
    12421 cgcccaacag caaggtacgc ttgaccttta ggcggtcttc gtcctccacg cgctgcccca
    12481 gcacgaccca cctgtcgcgc acccccgctt ccgcgagcag ctcctcctgg ttcagggtcc
    12541 agccgagaag ggtgcgtata tccgcttgcg tctgaggggg caggctgtcg atgcgccggt
    12601 aaccttcgag tagcaggtac aacctgccta ccgcggcgag catgcgctcg gcccacccat
    12661 ccccggactg ggttatggcc gccatctcct tcaccaggcg ggccgcgccc ggagcctggg
    12721 catccaccag gcgagcagcc gtccgctccc agtacgtgaa aggctgggtc tgggccgagg
    12781 ccaggccgtt acgcaccagg tcgtccaacc atgtctcaag ctcttgcaga ccaagtctaa
    12841 cacgcttctc acgctgttcg cttctacggt tctgctttgg ctcttgcctg gtcggccggc
    12901 tttccggggc ggctgtaccc tgctgttccg tgccgggggc tttgtccgca ggggcctgtt
    12961 ttgtcttctg tgcgcgctgg gcacgcgtat ccaaccagtc taccacccac gcgggggccg
    13021 cggcttctgt aaacaggctc ggcttgtcgg caaggagcag aaacaggccg attccatgtt
    13081 tgcatgggaa cttgcggctt ggacaggtgc aacgaaaagc aggctccgaa aggtcaatct
    13141 gcgtcctgta cgggctggaa ccgctgccct ggcactcccc ccatacaacg gacttactag
    13201 cctggcagcc cagagtcggc catttccgag gtgtggcaag ctccctacct gactttacag
    13261 aacctgggtc cggcgcaagc gcgattatct gctcggctgt gagaggagaa ttcatttggg
    13321 ttggcggaat ggtgtgctag gatgcgttcg agacgtatgc cagatctgct ttgtgagaat
    13381 gtatcgaata ctcaacctgc atagtataac tgccattgct tgaggacgga ataggttacc
    13441 aactatacac gcctagcatg ttattgccgt tgatgcctgg attgtctaat tggacatgat
    13501 tgcatataga ccttaccaga cacagtctaa gaaacacaac accaaaagtc ctgctgggat
    13561 aaaccagatg tagacctagt aatcttgctc agaaaagtct atactgactc actattccat
    13621 tcttgcttgt agcatagttt tcggccaatc cataggagag ctaacaatgt caaagcgtcg
    13681 accagatgga gttgaaggcc ctaccgaaac ggcaaataac cgaagctccg aggcctaagc
    13741 aaggggtcac cagctaatgg ttggcaagaa tatagagaca ttgtgaaatt ataggagggg
    13801 aacaatcatg ctagaagtgg cagctgttgt catcaatacc attgcggcta agatagcaga
    13861 gaaaatgcta ag
//
LOCUS       DASRWZ010000400         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1130490, whole genome shotgun sequence.
ACCESSION   DASRWZ010000400 DASRWZ010000000
VERSION     DASRWZ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130490"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /gene="lepA"
                     /locus_tag="VFH60_06200"
     CDS             <1..1166
                     /gene="lepA"
                     /locus_tag="VFH60_06200"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET6313408.1"
                     /translation="RIKLMATEATYETTQLGAFTPANSPREALEAGEVGYIIAGIKEL
                     QAAKVGDTVTLIKPGSGGAAFTASEPLPGFKEVQPQVFAGLYPVEANQYDSLRDSLEK
                     LKLNDASLHYEPEVSQALGFGFRCGFLGLLHMEIIRERLEREYNLALLVTAPNVEYKV
                     TKTDGTAMVVDNPADMPQAGNIASIEEPWMEISIVTPTRYIGPIMDLVTARRGSYKKM
                     DYLDEERVVLVYEMPLGELIVDFYDQLKSRTQGYASLDYQLAEYRQADLVKLDILVAG
                     SPVDALSIITHREKAHYQGRQLVDRLRRLIPRQLFEVPIQAAIGSKIVARETISAMRK
                     NVLAKCYGGDITRKRKLLEKQKEGKARMKMVGNVEIPQEAFMAVLALEEPAKS"
     gene            1189..>2357
                     /locus_tag="VFH60_06205"
     CDS             1189..>2357
                     /locus_tag="VFH60_06205"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET6313409.1"
                     /translation="MEELNPYEVLHVPRDAGVDAIEDAYDSLFDEYESRAQWGDKSAT
                     DMLHKLNEARDRLVDPELRAQVDAALTHKPLSSTIPQNPAQAAPAAEQPKATPTPQAR
                     QAEQTTSRSTTAEAERMRVRRRSQARPRAVQPVATSSRLPYVLVSALAVALAGALLFL
                     LLRPSQPAGGNEDRGAILATVNGAPIYDQDFQERLEIDRNTALADPLFSPFFNNFEGI
                     TGTRALNILKYDALDKLINLEVILQQAHKEGLYPQEAQQQSIINEAKAREVPAGQTFE
                     AFLQQRNITAGQYNRKVISNVVYTLMANEHLPKEGDANTRTDGFIKWICDTRKNYDVR
                     ILMTLTEPAGSTAPPVENVPCTSGLPTDLPLPGVEEAPPEPEQTAIPPIDPGTVPG"
BASE COUNT          541 a          794 c          676 g          346 t
ORIGIN      
        1 accgcatcaa gctgatggcc acggaggcca cctacgaaac gacacagctc ggcgccttca
       61 cgccggccaa ttcgccgcgc gaggcactgg aggcgggcga ggtcggctac atcatcgcgg
      121 gcatcaagga gctgcaggct gcgaaggtgg gcgacaccgt cacgctgatc aagcccggct
      181 cgggcggcgc cgccttcacg gcgtccgaac cgctgcccgg cttcaaggaa gtgcagccgc
      241 aggtgttcgc cggcctgtac ccggtggagg ccaaccagta cgactcgctg cgcgacagcc
      301 tcgagaagct caagctgaac gatgcgtcgc tgcactacga gcccgaagtg agccaggcgc
      361 tcggcttcgg cttccgttgc ggcttcctgg gcctgctgca catggaaatc atccgcgagc
      421 ggcttgagcg cgagtacaac ctcgcactct tagtcaccgc ccccaacgtg gagtacaagg
      481 tcacgaagac cgacggcacg gcgatggtgg tggacaaccc ggcggatatg ccccaggccg
      541 ggaatatagc cagcatcgaa gaaccctgga tggagataag catagtcacc cccacgcgct
      601 acatcggccc catcatggac cttgtcaccg cccggcgcgg ctcatataaa aagatggact
      661 acctggacga agagcgcgtt gtgctcgtct acgagatgcc gctgggcgag ttgatagtgg
      721 acttctacga ccagctaaaa tcgcgcaccc agggttacgc ctccctcgac taccagcttg
      781 ccgagtacag gcaggccgac ctcgtgaagc tggacatcct ggtggccggg tcgcccgtgg
      841 acgcgctcag tatcatcact caccgcgaga aggcccacta ccagggccgc cagcttgtag
      901 acaggctgcg tcgtctcatc ccccgccagc tattcgaggt gcccatccag gcggctatcg
      961 gcagcaagat tgtggcgcgc gaaaccatct ctgcgatgcg caagaacgtg ctcgccaagt
     1021 gctacggcgg cgacatcacc cgcaagcgca agctgctgga aaagcagaag gaaggcaagg
     1081 cccgcatgaa gatggtgggc aacgtggaga taccccagga agcgtttatg gcggtgctcg
     1141 ccctcgaaga gcctgccaag agttagataa caccaggaga gcagcagctt ggaagaactc
     1201 aatccgtatg aggtcttaca cgtcccccgc gatgccggcg tagacgctat cgaggacgca
     1261 tacgacagcc tcttcgatga gtacgagtcg cgcgcgcaat ggggcgacaa gagcgcgacc
     1321 gatatgcttc acaagctgaa cgaagctcgt gaccggctcg tagaccccga gttgcgtgcc
     1381 caagtagatg ccgcgctcac ccacaaaccg ctatcctcca caatcccaca aaaccccgcg
     1441 caggcggccc ctgcggcgga acaacctaaa gcaaccccta ccccccaagc acggcaggca
     1501 gagcaaacca ccagccggag cacaacagcc gaagcggagc gcatgagggt acgccggagg
     1561 tcgcaggcga ggcccagggc cgtgcagccc gttgccacat cttccaggct gccctacgtg
     1621 ctggtaagcg cgctggccgt cgccctggca ggcgcgctcc ttttcctcct gcttagaccg
     1681 tcgcaaccgg cgggcggcaa cgaagatagg ggcgctatcc tggcgacggt gaatggcgcg
     1741 cccatatacg accaggactt ccaggagcgc cttgagatag acaggaatac cgcccttgcc
     1801 gaccccctct tctccccctt cttcaacaac tttgaaggta tcacgggcac gcgcgccctg
     1861 aacatcctca agtacgatgc cctcgacaag ctgataaacc tcgaagtaat cttgcagcag
     1921 gcacacaaag agggccttta cccgcaagaa gcccagcagc agagcataat caacgaggcg
     1981 aaggccaggg aggtacccgc cgggcagacc ttcgaggctt tcttgcaaca gcgaaacata
     2041 accgcgggcc aatacaaccg caaggtcatc tcaaacgtcg tctacaccct catggcaaac
     2101 gaacacctgc ccaaagaggg cgacgctaac acccggaccg acgggttcat caagtggata
     2161 tgcgatacgc gcaagaacta cgacgtgcgc atcctcatga ccctcactga accggcaggc
     2221 tccacagccc cgcccgtaga aaacgtccca tgcaccagcg gcctccccac cgacctgccc
     2281 ctccccggcg ttgaggaggc accgcccgaa ccggagcaaa ccgcaatccc gcccatcgac
     2341 ccaggcaccg tccccgg
//
LOCUS       DASRWZ010000401         1991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_377325, whole genome shotgun sequence.
ACCESSION   DASRWZ010000401 DASRWZ010000000
VERSION     DASRWZ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377325"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..>1991
                     /locus_tag="VFH60_06210"
     CDS             251..>1991
                     /locus_tag="VFH60_06210"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6313410.1"
                     /translation="MAATKTWSSLLLGVLLGAALLGAWLAGWHLLVDAAGLFAPVAAL
                     VGVTLWLLAQRSPLARSATIRVALNTAGVGAMALAVAASLSLGAFLPAGLAEWVSPVL
                     GRFGGWAALLILAIGMCLGVALPVGRGSGLLPHLAAGLGFGGIWVGSQVERQATFPPP
                     PTTASLLSFRFDLLLLYGLLFAAVPLAAWLFERLKRGRTSSSGPTTSQAAEWRRLFPF
                     AIRWALLMTMLGLAGSLLLGSEGWYRFALLALALVVAALVVVARDRSSEASNPARHAG
                     KSLLRLAGWLVAGLLISGLVFFTTSDDLEIVLLRNNIEWRTMQFWRGVVGDERFDARP
                     VGVLSGRVNNRQGEPVEGAAVIVSGVSGQVYSGRTAPDGSYKIQGVAAGNYLPMSVAP
                     EHRQAAASTFFGRVATVRPGQETGSINFVLERNRPLELASEVEMSLGQPGEVTVPNPQ
                     PSTAVRRAFTFRNGGLLLDGGLVHEPLDAMGPGPFPILLIVYPGEASAWEGVSVPLAT
                     NGYVVVSYFPRRLLDLEGDVDDLRVLTSLAAGGRLSGRGETSRMVLVGGSVSTVYTYL
                     LAREMEGSRVEEQV"
BASE COUNT          324 a          589 c          680 g          398 t
ORIGIN      
        1 ccccgatgtt gccctattct ctagcacatg gcaccgtaca ggcgtaaaga aaggtgatat
       61 ttgggtgctg agggacgaga ttcttcgctg cgctcagaat gacaaggggg gaacttggtc
      121 ccacgtaggt ggcagtttgc agataggccc aagagatacc atctagctgc ttgctccgcc
      181 gtattcgaaa cgcagcccag gtgctataat cagagccgag tattggcaac agcagtagga
      241 gagattcggc atggcagcga caaagacctg gtcgtctcta ctgttgggcg tcttgctggg
      301 ggcggctctg ctcggtgcgt ggctggccgg ttggcacctg ctggttgacg cggcaggtct
      361 gtttgctcct gtcgctgctc ttgtcggggt aaccctgtgg ttgctcgccc agagaagtcc
      421 gctggcgagg tccgctacga tacgtgtcgc tctcaacacc gccggtgttg gggctatggc
      481 cctcgcggtg gcggcttcgc tatccctggg agcgttccta cctgccgggc tggcggagtg
      541 ggtcagcccc gtgttaggca ggttcggcgg gtgggcggcc ctgcttatcc tggccatagg
      601 catgtgcctt ggggtggcac tccccgtggg caggggcagc ggccttctgc cccacctggc
      661 agcgggactg ggctttgggg gcatctgggt cggaagccag gtcgaacggc aggccacgtt
      721 cccaccgcct cccacaacgg cctcactact ttccttccgt ttcgatctct tactcctcta
      781 tgggctgctc ttcgccgcgg tgccgctcgc cgcctggttg ttcgaacgcc tcaagagggg
      841 tcgtacttca agctccggcc ccacgacctc acaagctgcc gaatggcgca ggctcttccc
      901 cttcgcgata cgttgggctt tactcatgac aatgcttggg cttgcgggca gcttgttgct
      961 aggctcggag gggtggtaca ggttcgcgct gttggccctg gccctcgtgg tggctgcgct
     1021 ggtagtcgtg gcgcgagaca ggagcagcga ggcttcaaac ccggcacggc acgccggtaa
     1081 gtcgctcttg cggctggctg gctggctcgt ggcggggcta ctaatatcgg ggctggtgtt
     1141 tttcacaacg tccgacgacc tggagatcgt gttgctcagg aacaacatag agtggcggac
     1201 gatgcagttc tggcgtggag tggttggcga cgagcgtttc gacgccaggc cagtgggagt
     1261 cctgtcaggg agggtgaaca acaggcaagg ggagccggtg gaaggggccg ccgtaatcgt
     1321 atcaggcgtt agcgggcagg tgtacagcgg ccgcaccgcc cccgacggca gctacaaaat
     1381 tcagggcgtt gcggcgggca attaccttcc catgtccgtg gcccccgagc accggcaggc
     1441 ggcagcaagt acatttttcg ggcgcgtcgc aacggtcaga ccagggcagg aaaccgggag
     1501 catcaacttc gtactggagc gcaaccggcc tcttgagctt gccagcgaag tagagatgag
     1561 cctgggccag cctggggagg tgaccgtccc gaacccgcaa cccagcaccg ccgtgcgccg
     1621 cgccttcacc ttccgcaacg gcgggctgct actggacggc ggcctggtgc acgagccact
     1681 tgacgcaatg gggcctggcc ccttccctat cttgctgatc gtctacccgg gggaagccag
     1741 cgcctgggaa ggggtatcgg taccgctggc cacaaacggc tacgtagtgg tcagctactt
     1801 cccgcgccgc ttgctcgacc tcgaaggcga cgtggatgac ctgcgggtct tgaccagtct
     1861 cgcggcgggc ggcaggctct ccgggcgcgg cgagacttcc cggatggtgc tggtcggcgg
     1921 cagcgtctcc accgtctaca cctaccttct ggcaagggaa atggaaggga gccgggtgga
     1981 ggagcaggtg c
//
LOCUS       DASRWZ010000402         7858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_856895, whole genome shotgun sequence.
ACCESSION   DASRWZ010000402 DASRWZ010000000
VERSION     DASRWZ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856895"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFH60_06215"
     CDS             <1..553
                     /locus_tag="VFH60_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313411.1"
                     /translation="GGSEGQDSGQAGGNQQSAGGGSAGGASTDSGEQGTLGGSINGPV
                     GGAGGAISQVPNPAGQGNEQEDGNSTSGGSGDGSADEGDEQVYVPETAGMEEGAEGSV
                     ETDTGDEDANTEGVEGRPGEGEGNGAQLSSSSGGGGAVRVQTPYREVIAEYARQATEA
                     IERAYVPTDAKEYVREYFSELGK"
     gene            750..1667
                     /locus_tag="VFH60_06220"
     CDS             750..1667
                     /locus_tag="VFH60_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6313412.1"
                     /translation="MVGQRNLIHQVLVALIAGGNVLLEGVPGLGKTVLVRTLAEVIDC
                     EFSRVQFTPDLMPADIIGTNILNEDEHGRRAFEFQRGPVFANLLLADEINRATPKTQS
                     ALLEAMQEGTVSVARTIYELPKPFFVLATQNPLEMEGTYPLPEAQLDRFFFKLDVGFP
                     TAAELIAISDRTTSGVEADLGRVARAQDVLDMQHLARQTPIATHVTAYAVRILRATHP
                     NNSEATALVKRYVRYGGSPRALQAMILAGKIMALLDGRYNVAFRDIRTAALPALRHRL
                     ILNFDGEAEGISPDDIISALLEETPEEAQ"
     gene            1765..3768
                     /locus_tag="VFH60_06225"
     CDS             1765..3768
                     /locus_tag="VFH60_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6313413.1"
                     /translation="MGLFEPVALLALPLLGVIVALYLLRFRRPSAPVGSLQLWDNLTR
                     DREANALWQRLQMSGLMLLQLLAMLALILALARPWLPAESISGQNAILVIDTSASMGA
                     MDGATDRRISRLEAALDKARSLIDDLPQGAISAIITSDEHAAVLVPPTDDKARLRDAL
                     ARLKPQPTGTNMSEAIQLASVLAQRQANSTVWVLSDGNFPAVGGEGQEVNAEVRFFPV
                     GSSASNQAVTAISVDHRAGSLGLFVQIVNADTLTVTRRLDLLVDDAPWNARTVSLGPG
                     ETQEVLVDDLPIEGRVVAAHLAGVDELEIDDTAWTVNRASVPANVLMVTEGNRFLELS
                     LALLPNVTLYKVAPADYDPGATLDGLPFDLTVLDAGVVSSTLSRLPDGNMLIFEPRAS
                     SPLFDVGEELVAPQPSFSLLDSGGLASSGNESGRDPLLRFVDLSNLHIAHAAQIKVPV
                     WGRVVLGSDKGPLIIVGEEGGRKVGVVAFDLQQSDLPLQTAYPLLMRNLVTYLLPDPT
                     GGVPVAATPRSIVGIDAVDHTTDRVLVEDPSGKEWTYGLAGDRRRVAFAETRLPGVYY
                     VTQFAEEEIVAQEAFVVNLASRDESAVRPNPRPGLPAGTGVSNAGAGTQQVSPFRREL
                     WPYVAVAAFVVLLVEWFVAQRITIRRVFLEWRSRRAQRRLGNI"
     gene            3778..6477
                     /locus_tag="VFH60_06230"
     CDS             3778..6477
                     /locus_tag="VFH60_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6313414.1"
                     /translation="MDFPFNVSEPRALILLLTLPPVVWLGVLTARARPRDRARIAAST
                     VLRTLILTLLTLAIAGTQLIATGGPLNVVFLVDRSASVSDSSRQASFDYVRQAIRSMG
                     PDDRAAVVLFGETAVLDRALSADTEWVSLGKEPPGLATDIADAIQVGTALFPEGGARR
                     MVLLSDGVETVGEARKIAAGDALAGAQLSVVPIGGTARNEVAVDRVVSPNSVPAGQQI
                     PVRVLVRSNSDRAASVTLFDGETPVETQDVQLKAGDNVVTFGTEAREQGFHVLKARVD
                     SVDDRFSENNEALSFTIVKRPPSVLILATTETDALPFKNALESSGIEAQVVEPVALPR
                     DPANLDKFDAVVLANVSAESLEIEGQQVLQSYVRDLGRGLIMVGGDLSYGAGGYARTP
                     IEEVLPVRMDARTSEERASLAMTFVIDKSGSMGRCHCGGSQQFDPTLRTEFGDSKIEL
                     VKRAISKSTALLNSNDQVGVVTFDEQPNWLARLQPLSDLGEQRLEQLLLPVEAEGDTD
                     MFGALSQSVDALEGSNAQLKHIVLLGDGWTKRADFDPLLSRMSASNITLSTVGVGDGA
                     YELMNELATRGGGQYYQADDVRTVPDVLLKETIRLAGSYYVEEALTPVVARASPILNG
                     IDTGAMPPLLGYNASTLKPDADLILRSPRGDPLLAQWQYGLGRAVAWTSDAKGQWAAD
                     WVAWPQFGKFVGQMVGWTLPAQENVALEATFSARPDSWGGGQELGVRVDTVTATGAPR
                     GFMPTPLVITGTGGLTTTVVVSPRSPGVYDGVAHGLSPGADAATVEQRDANTGELLER
                     LDTGFVVPYPSEYRLSEDTAQGAQALLSDLAQLGGGKVLEITQPSLAFSHDISPEPRR
                     LALWPWLLLGAILLFPLDVAVRRVSLTPRELWRLLRGRAPENV"
     gene            6470..7243
                     /locus_tag="VFH60_06235"
     CDS             6470..7243
                     /locus_tag="VFH60_06235"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HET6313415.1"
                     /translation="MSERRITVDLNADVGESFGAWPMGHDAEMMPYITSANVACGGHA
                     GDPNVMAATVRLAAKYGVSVGAHPGYPDLPGFGRRVMAFSPEEIRHFVLYQVGALWAI
                     ARAQGVALRHVKPHGALYNVAARDGQVAAAIAAAVRDFSRDLPLVCLAGSVAEGVSRE
                     MGLLTLSEGFADRAYEPDGALRDRKLPHSVHHEPGVVAEQVVSLASGSVRAVDGSEIA
                     VRVDTICLHSDTPEAALFARTARQALERAGYAVSAAKIG"
     gene            7245..>7858
                     /gene="pxpB"
                     /locus_tag="VFH60_06240"
     CDS             7245..>7858
                     /gene="pxpB"
                     /locus_tag="VFH60_06240"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HET6313416.1"
                     /translation="MDAAPQARIQQMGEGALLVTLGTGISPQVNGRVHALAARLRLLK
                     LRGVIDIVPAFASVLVTFDDTRIDEAALGEQIKRLLAAERPVDGTAPRRHEIPVVYGG
                     EHGPDLERLAASHGLSAAEVVKRHAAPVYRVYFLGFMPGFAYLGGLDPSIASPRLDSP
                     RVRVPAGAVGIAGDQTGIYPLASPGGWHLIGRSNVPLWDIGRDPP"
BASE COUNT         1400 a         2490 c         2680 g         1288 t
ORIGIN      
        1 gggcggaagc gaggggcagg acagcggcca ggcgggcggc aaccagcagt cggccggagg
       61 cgggtcggcg ggcggcgcga gcaccgattc gggcgagcag ggcacgcttg gcggatcgat
      121 aaacggcccg gtgggcggcg cgggaggcgc gattagccag gtccccaacc cggccgggca
      181 aggcaacgag caggaggacg gtaacagcac gtccggcggc tccggcgacg gttcggcgga
      241 cgagggcgac gagcaagtct acgtgccaga gaccgccggg atggaggagg gagccgaggg
      301 gtccgtggag acagataccg gcgacgagga tgcgaacacc gagggggttg aaggtcgccc
      361 cggcgaaggc gaaggcaacg gcgcgcagct atcgtccagc tcagggggtg gcggtgcggt
      421 tcgagtgcag acgccctacc gcgaggtgat agccgagtac gcacgccagg cgacggaggc
      481 tatagagcgt gcctacgtgc ccacggacgc caaggagtac gttagggaat atttctcgga
      541 gcttggcaag taagcggtaa aggtaagaag ctgggttgcg ggcaatggtg gcccgccggt
      601 gcacgaattt tctcaaggag cacctagtgg cattgacaga gagagacacg agcgctgtcg
      661 gtgtggggtc acacccggcg cgtcaggagc taagcccgga ggcgtttcgg gcgcgggccg
      721 tggctatcga gcaagaggtc ggcaagatca tggtgggcca gcgcaacctg atccaccagg
      781 tgctggtagc gctgatcgcg gggggcaacg tcttactcga aggtgtgccc ggccttggca
      841 agacggtgct cgtgcggacc ctggcagagg tcatcgattg cgagttcagc cgggtgcagt
      901 tcacccccga cctgatgccc gccgacatca tcggcacgaa catcctcaac gaggacgagc
      961 acgggcgcag ggcgttcgag ttccaacgtg gacctgtgtt cgcgaacctg ctgctggccg
     1021 acgaaatcaa ccgggccacg cccaagactc agtcggcgct gctcgaagca atgcaggaag
     1081 gcaccgtatc ggtcgcccgt acgatctacg agctgcccaa gcccttcttc gtgcttgcca
     1141 cgcagaaccc cctcgaaatg gaaggcacgt acccgctgcc ggaggcccag ctcgaccggt
     1201 tctttttcaa gttggatgtt ggcttcccca ccgccgccga gctaatcgct atatcggatc
     1261 gcacgacatc cggggtagag gcggacctgg gccgcgtggc acgtgcccaa gacgtgctcg
     1321 acatgcagca cctggcgcgg cagacgccca ttgccaccca cgtcaccgcc tatgccgtgc
     1381 gcatcctccg ggcaacacac cccaacaact cggaggcgac cgccctggtc aaacgctacg
     1441 tgcgctatgg cggctcaccc agggcgctgc aagctatgat cctggcgggg aagatcatgg
     1501 cgctgctaga cgggcgctac aacgtagcct tccgcgacat caggaccgcc gccctccccg
     1561 cgctgcgtca ccgcctgatc ttgaacttcg acggcgaggc cgagggtata tccccggacg
     1621 acatcatctc ggcgctactc gaagagacgc ctgaggaggc tcagtagccc tgtgcgagca
     1681 gaggcgcacg caccggccgg ggccttctcc cgtcacgcct ggcctcaact gcccggttta
     1741 acggggcggc gctaggtggg cgtgatggga ctgttcgagc cggttgctct gttggcgctg
     1801 cctttgctgg gcgttatcgt ggcgctctac ctgttgcgct ttcgccgccc cagcgcaccc
     1861 gtaggcagcc tgcaactgtg ggacaacctg acgcgggacc gcgaagccaa cgccctctgg
     1921 cagcgcctgc aaatgtccgg cctcatgctg ctccagttgc tcgcgatgct ggccctgatc
     1981 ctcgccctgg cgcggccctg gctgccggcc gagagcatca gtggccagaa cgccatcctg
     2041 gtgatagata cctctgccag catgggggct atggacggcg cgacggaccg ccgcatcagc
     2101 aggctcgaag cggccctgga caaggcgagg tctctaatag acgacctgcc gcaaggggcc
     2161 atctccgcca tcatcacgtc tgacgagcac gccgcagtac tagtgccgcc caccgacgac
     2221 aaggcacgcc tgcgagatgc actggcccgg ctcaagccgc aacccaccgg gacgaacatg
     2281 tccgaggcta tacaactagc ctccgtgctc gcgcagcgcc aggcaaacag cactgtctgg
     2341 gtgctgtcgg acggtaactt cccggcggtc ggcggtgagg ggcaggaggt aaacgcggaa
     2401 gtgcgcttct ttccggtggg cagcagcgcg tccaaccagg cagtgacggc gatttccgtg
     2461 gaccaccggg cgggcagcct tggcctcttc gtgcaaatag tcaacgccga tacgctgacg
     2521 gtgacccgca ggctcgacct gctggtggac gatgccccct ggaacgcgcg caccgtgtcg
     2581 ctcgggccgg gagagacgca agaggtgctg gtggacgacc tacccatcga ggggcgcgtg
     2641 gtagccgccc acctggcggg tgtggacgag cttgagatag acgatacggc ctggacggtg
     2701 aaccgtgcca gcgtgcccgc caacgtgctc atggttacgg aaggcaacag gttcctcgag
     2761 ctgtcgcttg cgctcctgcc gaacgtgacc ctctacaagg tggccccggc cgattacgac
     2821 ccgggtgcga ccctcgacgg cctgccgttc gatctgacgg tgcttgacgc gggcgtggta
     2881 tcctccaccc tgagccgcct gcccgacggc aacatgctca tattcgagcc gcgcgcctcc
     2941 tcgcctctct ttgatgtcgg ggaggaactt gtggcccctc agccctcctt ctcgctcctt
     3001 gactccggcg gcctggcctc ctccggtaac gaatcgggac gcgatcccct gctccgtttc
     3061 gtggaccttt caaacctgca catagcacac gccgcgcaga taaaggtgcc ggtgtggggc
     3121 cgcgtggtgc tgggtagcga caaagggcct ctcatcattg tgggggagga gggcggacgc
     3181 aaggtgggag tggtagcgtt cgacctgcag cagagcgacc tgcctttgca gacggcatac
     3241 cccctgttga tgcgcaacct cgtcacatac ttgttgcctg acccgacggg aggcgtgccg
     3301 gtcgcggcga ctccccgttc gatagttggg atagatgccg tcgatcacac gaccgatagg
     3361 gtgctggtcg aggacccgtc tgggaaagag tggacctatg ggctggcagg cgaccggcgg
     3421 cgggtggcct tcgcggagac aaggctcccc ggcgtgtact atgtgaccca gttcgcggaa
     3481 gaggaaatcg tggcgcagga ggctttcgtg gtgaacctgg cgtcgcggga cgagtctgcc
     3541 gtgcgcccca acccacgccc cggcctgcct gcgggcaccg gcgttagcaa cgcaggggca
     3601 ggcacgcaac aagtcagccc attccggcgt gagctttggc cctacgtggc ggtggctgct
     3661 ttcgttgtgc tccttgtaga gtggttcgtc gcccagcgca tcacgatacg ccgtgtattc
     3721 ctcgaatggc gcagccgcag agcgcagcgc agactcggca atatctgaga ggcagcaatg
     3781 gacttcccgt tcaacgttag cgagccacgc gctctcatat tgctcctcac gctcccgccg
     3841 gtggtgtggc tgggggtgct taccgcgcga gcacgtccca gagaccgcgc gcgtatcgcc
     3901 gcgagcaccg tcctccgcac gctgattttg actctgctca ccctggcaat cgcgggcacc
     3961 cagctaattg cgaccggagg gccgttgaac gtggtcttcc tggtggaccg ctcggccagc
     4021 gtatcggact ccagcaggca ggcctccttc gactacgtac gccaggccat ccgctcgatg
     4081 ggacctgacg accgcgcggc cgtggtgcta ttcggtgaga cggcggtgct cgaccgcgcc
     4141 ctgagcgccg ataccgagtg ggtgtcgctc ggcaaggagc cgccggggct tgccaccgac
     4201 atagccgacg ccatccaggt gggcacggcc ctcttcccgg agggtggcgc gaggcgcatg
     4261 gtgctgctca gcgacggcgt ggaaactgtg ggcgaggccc gcaagatcgc ggcaggggac
     4321 gcgctcgcgg gcgcgcagtt atcggtggtg cccataggcg gcaccgctcg caacgaagta
     4381 gccgtggaca gggtggttag ccctaacagc gtcccggccg ggcagcagat accggtgcgc
     4441 gtgctcgtac gcagcaatag cgaccgggcg gcaagcgtca ccctgttcga cggagaaacg
     4501 cccgtcgaaa cgcaagacgt gcagctgaag gcgggtgaca acgtcgtgac tttcggtacg
     4561 gaagcgcgcg agcaggggtt ccacgtgctc aaggctcgcg tcgattccgt ggatgaccgc
     4621 ttcagcgaga acaacgaggc tctgtccttc acgattgtaa aacggccgcc gtccgtgctg
     4681 atactggcta ccaccgagac cgacgcactg ccattcaaga acgcgctgga gtcgagtggc
     4741 atcgaggccc aggtcgtaga gcctgtggcc ttgccccgcg accccgctaa cctggacaag
     4801 tttgacgccg tcgtgctcgc caacgtatcg gctgaaagcc tggaaatcga gggacagcag
     4861 gttctacaga gctacgtgcg cgacctggga cgcgggctta tcatggttgg cggcgacctg
     4921 agctacggtg cggggggcta cgcacgcacg cccatcgagg aggtgctccc ggtgcgcatg
     4981 gacgcgcgca cctcggagga gcgggcttcc ctcgcgatga cgttcgtgat agacaagagc
     5041 ggcagcatgg gccgttgcca ctgcgggggc agccagcagt tcgaccccac gctccgcacc
     5101 gaatttggcg acagcaagat agaactggtg aagcgagcga ttagcaagtc caccgcgctg
     5161 ctgaactcca acgaccaggt gggcgtggtt actttcgacg agcaacccaa ctggctggcg
     5221 cggctccaac cgcttagcga cctgggagag cagcgtctgg aacagttgct actacccgtc
     5281 gaggcagagg gcgacacgga tatgttcggc gccctttctc agtcggtgga cgcattggag
     5341 ggcagcaacg cgcagctcaa gcatatcgtg ctgctgggag atggctggac caagcgtgcg
     5401 gacttcgacc ccttgctctc ccgcatgtcg gcaagcaaca taaccctctc gacggtgggt
     5461 gtaggtgacg gcgcgtacga acttatgaac gaactcgcca ccaggggagg cggccagtac
     5521 taccaggccg acgacgtgcg aactgtgccc gacgtactac tgaaagaaac gatccggctg
     5581 gcaggctcct attacgtgga agaggcgctt acccccgtgg tggcccgtgc cagccccatc
     5641 ctgaacggga ttgacacggg cgcgatgccc ccgctactcg gctacaacgc aagcacgctg
     5701 aagccggacg ccgacctgat tctccgttcc ccgcgcggcg acccactcct cgcgcagtgg
     5761 cagtacggcc tcggccgggc agtcgcctgg acctcagacg ccaagggcca gtgggcggcc
     5821 gactgggtcg cctggccgca gttcggcaag ttcgtgggcc agatggtggg ctggactctg
     5881 cctgcccagg agaacgtggc acttgaggct accttctcgg cgcgtccgga ctcgtggggc
     5941 ggcggtcagg agttgggcgt acgcgtggac acggtgaccg ccacgggtgc gccgcgcggc
     6001 ttcatgccca cccccctggt aataacgggg accggcggcc tcaccacgac ggtcgtagta
     6061 tccccccgct cgcccggcgt gtacgacggg gtggcacacg ggctaagccc aggagctgac
     6121 gccgccacgg tggagcagcg ggacgccaac accggcgagc ttctggagcg cctcgatacg
     6181 ggcttcgtgg tgccttaccc gagcgagtac cgcctgtcgg aggacactgc ccagggcgcg
     6241 caggccctgc tatcggacct ggcgcagcta ggaggcggca aggtgctcga aatcacgcaa
     6301 cctagcctgg ccttcagcca cgacatatcc cctgagccac gaagattggc gttgtggccg
     6361 tggctcctgc tgggcgcaat tctgctgttt ccgctggacg tggcggtaag gcgtgtcagc
     6421 ctgaccccgc gcgaactgtg gcgtttgctg agggggcgcg cacctgaaaa tgtctgagcg
     6481 ccgaattaca gtcgatttga atgcggacgt gggcgagagt ttcggtgcct ggccaatggg
     6541 ccatgacgcc gagatgatgc catacatcac gtccgcgaac gtggcttgtg gcgggcacgc
     6601 gggcgaccca aacgtcatgg ccgctacggt acgtctcgcg gcaaagtacg gtgtcagcgt
     6661 gggggcgcac cccggctacc ccgacctgcc ggggttcggg cggcgcgtga tggccttctc
     6721 gcctgaggaa atacgacact tcgtgctcta ccaggtcggc gcgctgtggg caatcgccag
     6781 ggcgcagggt gtcgcactgc ggcacgtcaa gccgcatggc gcgctttaca acgtggcggc
     6841 acgcgacggg caggtcgcgg cggccatagc ggcagccgtc cgcgacttct cgcgagacct
     6901 cccactggtg tgcctcgcag gttcggtggc cgagggcgtg tcgcgcgaaa tggggctatt
     6961 gaccctctca gaaggctttg cggaccgcgc atacgagccg gatggggcac tgcgcgaccg
     7021 aaagctgccg cacagcgtgc atcacgagcc tggcgtggtc gcggagcagg tcgtgtcgct
     7081 ggcgagtggg agcgtgcggg cagtagatgg cagcgagatc gcggtgcggg tggataccat
     7141 ctgcctgcac agcgacactc cagaagcggc cctgttcgcg agaacagcgc gtcaggcgtt
     7201 agagcgggcg gggtatgccg tctcggcagc caagattggc tgaagtggac gcggcaccgc
     7261 aggcgagaat ccagcagatg ggcgaggggg cgctactcgt tactctcggc accggcattt
     7321 cccctcaggt gaacggccgc gtacatgccc tggcggcgcg actgcggctc cttaagctcc
     7381 gtggcgttat cgacattgta cctgccttcg ccagcgtcct tgtcacgttt gacgacactc
     7441 gcatcgatga agcagcgtta ggagagcaga tcaagcggct cctggcggca gagcgacccg
     7501 ttgatggcac cgcaccacgc aggcatgaaa tacctgttgt ttacggcggc gagcacgggc
     7561 cggacctgga acgcctggcg gccagccacg gcctcagcgc tgctgaggtg gtgaagcggc
     7621 acgccgcgcc tgtttacagg gtctacttcc tgggcttcat gccgggcttt gcttacctgg
     7681 gagggctgga cccctccatc gcctcgcccc ggctggacag cccccgcgtg cgagtcccag
     7741 ccggggcggt cgggatagcg ggcgaccaga ccggaattta cccgctggca agccccggcg
     7801 ggtggcacct aatcggccgc agcaacgtgc cgctatggga cattggccga gacccacc
//
LOCUS       DASRWZ010000403         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1335956, whole genome shotgun sequence.
ACCESSION   DASRWZ010000403 DASRWZ010000000
VERSION     DASRWZ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335956"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VFH60_06245"
     CDS             <1..1195
                     /locus_tag="VFH60_06245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313417.1"
                     /translation="ELVMYRQLERLARRHEVTLATVCSQDQDSGGGIADLRRLGIEVH
                     TVTRRVPAGLERWKRRPGLALRWLRGHTPLPVIEFTLPAMQATINRLVAATAPELIQV
                     EYVWMAGYQFPPGVATVLTEHEVGLARARDDLQATVEAPRLRRLYALAEWRRWRRYEP
                     AACRKFARVQVFTSQDANTLLHHAPDLEPRLRVNPFGVDPPGAPEPAAEDENAVLFVG
                     GFAHPPNVDAALWLAREIMPLVWRDVPGAHLWLVGSNPPAELVALAGERITVTGWVPE
                     IEPYLQRAAVVVAPLRQGGGMRLKVLQAMSAHKAVVTTPLGAAGIAEEDSGPLVIAAS
                     AEDLARRTVELLANRHMRSELGARAREFTLARYSWEAYMGRLQEIYDEALAAHHSRGK
                     TPGQQ"
     gene            1192..2721
                     /locus_tag="VFH60_06250"
     CDS             1192..2721
                     /locus_tag="VFH60_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET6313418.1"
                     /translation="MSQFLKNSISGYVSSATIIAIGLLLTPLLVRQLGHQAYGVWVLV
                     TSLAIYLTLLDLGVADAVTRYVARARAVHDRDQVTAVVSTGFTVYLAGGVISLLVTLL
                     LVFLVLPTLNTPEEMLPTAQWLLMALGIGSMVILSCRVYDGALRGAQRYDLANALQIG
                     YSIFLAGGTALVLLLGGGLAEIAWVNLAASVLLAAGQTVVGRLAVPDARLKRHGVRRG
                     LARELVGFSSWVFLTNVSSRVYTHFDAVILAIFLPVRAITPYNLGYRVAALLGEGLYP
                     VLKLLFPIAAEMHMRREDEALHRLLVQGTKVALALSLAGGIILWFLGEALIRVWIGPG
                     LEEATPILYLFVVFFIISNTGQPVREVLKGQGRIRLLAMMAVADTVLNLGLSVVLVQF
                     FGAPGVAVGSLVPVVLVSLVLLRYTLTHQGIRLGEFIRTVIGPSVVPAAGTTALLLAL
                     SWLFPAAGWLRLIVSGLLAVITFGTLYLLLPGGAAEREQLLVRLRGMRGRSKVPAEET
                     P"
     gene            2718..3785
                     /locus_tag="VFH60_06255"
     CDS             2718..3785
                     /locus_tag="VFH60_06255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6313419.1"
                     /translation="MSRFDWRNTLKRGRLDARRYLAEPEAARSMQLSRYSEGTLRLRM
                     LRAVLLASRMPPRLLVLLSRPLRFILPESTWTEMVTDHAYWFGVRLGLERTGKWDQVR
                     GSGVPILLYHRIVPRLDPADPKYSLSLGRFRPQMRLLRLLGYRTISLRELVECHRSGK
                     FPPARSVVVTFDDGYRDNDAAARYMLTNGQSGIIFLVTGRMGHDRDWSLDGHRRMPLL
                     TWDEVRALKKAGFEFGSHTRTHPNLSALGADEACEEIDTARASIISELEDDGHLFAYP
                     YGGSSPEARTCVANAGYPAACGVRRGLSNMHDDLYYLKRITIYGNEKLLNFALRVVLG
                     DNPLDYLPWGRAREWMQRIES"
BASE COUNT          701 a         1139 c         1319 g          779 t
ORIGIN      
        1 cgaactggtg atgtaccgcc aactggagcg acttgcccgg cgacacgaag taacactcgc
       61 tacggtgtgc tcgcaggacc aggactccgg cggcgggata gctgacctgc gacggctcgg
      121 gatcgaggtc cacacggtca cgcgacgtgt ccccgcgggg ttggagcggt ggaagcggcg
      181 gccagggctt gcgctacgat ggttgcgcgg ccacaccccc ttgcctgtaa tagagttcac
      241 gctacctgcg atgcaagcga ccatcaaccg ccttgtagcc gccaccgctc ctgagctgat
      301 acaggtggaa tatgtttgga tggccggcta ccaattccca ccgggcgtag ccacagtgct
      361 gactgagcac gaggtggggc tggcgagggc gcgagacgat ttgcaggcca ccgtcgaggc
      421 tccgaggctg agacggctct atgctttggc cgagtggcgc aggtggcggc gctacgagcc
      481 agcagcctgc cgcaaattcg cgcgtgttca ggttttcacc agtcaggacg cgaacacctt
      541 gctacaccac gcgcccgacc ttgagccacg actccgcgta aaccctttcg gagtcgatcc
      601 gcccggcgca cctgaacctg cggccgaaga tgagaatgcc gtgctgttcg tcgggggctt
      661 cgcgcaccct cctaacgttg atgcagcgct gtggctggcg cgtgagataa tgcccctggt
      721 gtggcgagac gtgcctggcg cgcacctgtg gctggtgggc agcaatccac cggcggagtt
      781 ggtggcgctt gcgggcgagc gtatcacggt cacggggtgg gtgcctgaga tagagccgta
      841 cctccaacgc gcagcagttg tggtggcacc gctacgccag ggcgggggca tgcgtctcaa
      901 ggtgctccag gctatgtcag cgcacaaggc agtagtgacc acgccgttag gggcggcagg
      961 gatagctgag gaggactcag gcccgctggt aattgccgca tccgccgagg acctagcgcg
     1021 ccgcacggtc gagttacttg ctaatcgaca catgcgctcg gagcttgggg caagagcacg
     1081 agagttcact ttggctagat acagttggga agcctacatg gggcggcttc aggaaatata
     1141 cgacgaggcc ctcgccgcgc accatagccg tggcaagacg ccaggtcagc aatgagccag
     1201 tttctgaaga acagcataag cggttacgtg tcgagcgcga cgataatcgc gattgggctg
     1261 ctgctcacgc ccttgctggt ccgacaactt ggacaccagg cgtacggcgt atgggtgctg
     1321 gtgacctcgc tggctattta cctgacgctg ctagacctgg gtgtggccga tgcggtgaca
     1381 cgctacgtgg caagggcgcg cgccgtgcac gacagggacc aggtcaccgc ggtggtgtcg
     1441 acggggttca ctgtgtacct ggccgggggc gtgattagtc tgctggtgac gttgctgctc
     1501 gtgtttttgg tgctgccgac gctaaatacg ccagaggaga tgctacccac cgcgcaatgg
     1561 ctgttgatgg cgctaggcat cgggtcgatg gtgatactat catgtcgcgt ctacgacggc
     1621 gcgctcaggg gagcacagcg ctacgacctt gccaacgcgc ttcaaatcgg ctattcgata
     1681 ttcttggccg ggggcacggc actcgtcctg ctactgggag gcgggctggc agagatcgcc
     1741 tgggtgaacc tcgctgccag cgtgctactg gctgcaggtc aaacggtcgt cgggcggctc
     1801 gcggtgccgg acgcccgcct gaagcggcac ggagtacggc gcggcctcgc acgtgagctg
     1861 gtcggcttca gtagctgggt ctttttgacc aacgtatctt ccagggtcta cacgcatttt
     1921 gacgcggtga tactggcgat ctttctgccg gtgcgggcca tcacgcccta taacctgggc
     1981 tatcgggtgg ccgccctgct gggtgagggc ctctacccgg tcctcaagct gctctttccg
     2041 atagccgcag agatgcacat gcgccgggag gacgaagccc tgcaccgtct gctggtgcag
     2101 ggcaccaagg tagcattagc gctttccctt gcaggtggta tcatcctctg gttcctgggt
     2161 gaagcattga tccgtgtatg gatcggtcct ggcttagaag aagccacccc catactctac
     2221 ctgttcgtgg tgtttttcat aatctccaac accggccaac cggtacggga ggtgctgaag
     2281 gggcaggggc gcatcaggct gctggctatg atggcggtgg ccgacacagt gttgaacctg
     2341 ggtttgagcg tggtgttggt gcagttcttc ggagcgccgg gggtggcggt gggcagcctg
     2401 gtccctgtcg tgttggttag cctggtgttg ctgcgctaca ctctgacgca ccagggcatc
     2461 aggcttggcg agttcatacg cacagtaatc ggcccatcgg ttgtgcccgc ggcgggcacg
     2521 acagccctgc tactggcgct gagctggctg tttcccgcgg ccggctggct caggctcatt
     2581 gtgagtggcc tgcttgcagt tattaccttc ggcacgctct acctactctt gcctgggggt
     2641 gcggcggaac gcgagcagct actcgtgcgc cttcgaggaa tgcggggccg gtcgaaagta
     2701 ccggcagagg agaccccatg agcaggttcg actggcgcaa cacgttaaag cgcggacgcc
     2761 tcgatgcgcg ccgctacctg gccgagccgg aggccgcccg aagcatgcag ctctctcgct
     2821 acagcgaagg cacgctacgg ctgcgtatgc ttagagcggt gttattggcc agcaggatgc
     2881 ctccgcgatt gctcgtgctg ctcagtcgcc cgctccgatt tattctgccg gagagcacct
     2941 ggacggaaat ggtgacggat cacgcttact ggttcggagt gcgactgggg ctggaacgca
     3001 cgggtaagtg ggaccaggtc cggggcagcg gggtgccaat cctgctgtat caccggatcg
     3061 tgccccgcct cgatcccgca gaccctaagt actccttatc cctcggccgg tttcggcccc
     3121 agatgcgact cctgcgtctg cttggctatc gcacgataag cctccgggag ctggtggaat
     3181 gtcaccgctc cggtaagttt cctcctgctc ggagtgtggt agtgaccttc gatgacggct
     3241 accgggacaa cgatgcagcc gcccggtaca tgctcacaaa cgggcagagc ggcattatct
     3301 tcctggtgac cgggcgtatg gggcacgacc gggactggtc gctagacggc cacaggcgca
     3361 tgcctctcct gacgtgggac gaagtgcggg ccttgaagaa agccgggttc gagtttggct
     3421 cccatacgcg tacccaccct aacttgagcg cacttggggc cgacgaggca tgcgaggaga
     3481 ttgatacggc gcgggccagc attatcagtg agcttgagga cgacggccat ctgttcgcct
     3541 atccgtatgg aggctccagc cccgaggcaa ggacctgtgt agccaacgca ggctacccag
     3601 cagcgtgtgg ggtgcgtcgg ggcctcagca atatgcacga cgatctctat tacctgaagc
     3661 gcataacaat ctacggcaat gagaagttgc tgaatttcgc cttgcgggtt gtgctgggtg
     3721 ataacccgct tgactatctg ccctggggta gagcacggga gtggatgcag aggatcgaat
     3781 catgagcgcg atgcccgcga ttacggtggc aatagccacg ttggaccgtc cagacaacct
     3841 tgcgcgcgca ctggaggcgc tggcgagcgg tgaagtgctg ccccagcagc taattgtggt
     3901 tgaccagagc aagggtagcg agtcgcgcgc agtagttg
//
LOCUS       DASRWZ010000404         4048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_582955, whole genome shotgun sequence.
ACCESSION   DASRWZ010000404 DASRWZ010000000
VERSION     DASRWZ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582955"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFH60_06260"
     CDS             complement(<1..368)
                     /locus_tag="VFH60_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313420.1"
                     /translation="MSVSTVQKKARLCLAFVMAVVAIPLFPPSYAHLQGESRTFPETG
                     KSVRGKFLTYWNEHGGLPQQGYPISEEMQEKSDTDGKTYTVQYFERAVFEDHPENKPP
                     NDVLLSLLGNFLYKQNFPNGA"
     gene            950..>4048
                     /locus_tag="VFH60_06265"
     CDS             950..>4048
                     /locus_tag="VFH60_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313421.1"
                     /translation="MRSRIALDRRKLILAVLLLCLIVTAAFAVRLSQVQPGTALTRAD
                     VANSLYVAEPGESGHEESMMLSSRDEFLNARYTYPTGKADRRWVFEAAKQDKLIPSGI
                     PAGRVTYKGPSSPNAPDVLNANQWISIGPQPQQSETCSVCFPFVEVAGRVNDIVVDPI
                     TPTIAYIASDAGGVWKTTNCCRAATAWSPTMDDPYISTLAIGDLAIEPNGHTIYAGTG
                     DLRFGTFSFGSGGLLKSTDLGATWQVKGYDVFEPKYPQNAGVFPQYNSIGKVAINPHD
                     NNDLAVGTKLGMYFSYDAGDSWAGPCLPDPFPNQRQDITAIIAMTQTNTLADLFVAVG
                     ARGYSTTVQTNLAENGANSIYKTTWPSSGCPASWSHVSRADNGWPPGTGTGAPVYMNG
                     GNLLGRIDMAFSESNPDYIYAEVQAIKPGYGAIQRGGMLGIWRSTNRGESWEQRTTAQ
                     DLEDAQYACGGDCLADSLLGVCGDVAQNWYDQHIAVDPNDPDVIFFDNINVWKSTDGG
                     ETIKDLTCGYSSIQVPRPVHVDQHAITFLPDSSSRQLIGNDGGVYVTENANTEQPVYV
                     QLNTTLSTIEFYGGDISANFATSPAPFAVAGAQDNGSSSWQGAADVATSPRVWQQRIG
                     GDGMFARIEPVHGLRVYMEAQGGAIRRSDVGHQGPYQVACLNIDGIPNTPGCVPTNFQ
                     VDNPRVSFIFPYEIYTGVPAANGPGDECDQTEGCLHFIGGTYRVWENLDGLRDGSPAG
                     IPWYPTSPDLTKNTLADRSHINQLAFAFRTNKTAIAGTNDGNVWIGFGLGAGQGVTST
                     WKNLTGGNAVLPNRPILDVVLDPAVLSTTTNPVGFAAVGGFNENTPSQPGHVFKVTCG
                     SNCDTSTWENKSGNLPNIPVDAITVNPNYPSQVFAGTDWGVYYTNDINAASPMWYHFN
                     QGMPNVMIWDFAIDRGFTTLAAFTRSRGAYVWPLPSAPFEQTPTATVTGTPPATSTAS
                     PTAMTRTPDVPITYTPVPPTHTATPLATATMLACGSERLLYETFELGTLGAFTAKTET
                     DTG"
BASE COUNT          806 a         1316 c         1196 g          730 t
ORIGIN      
        1 gcgccgttgg ggaaattctg cttgtagagg aagttgccga gcagagagag cagcacgtcg
       61 ttgggcggct tgttctcagg gtggtcctcg aagacggcgc gttcgaagta ctgcaccgtg
      121 taggtcttgc cgtcggtgtc gcttttctcc tgcatctcct ccgagatggg gtagccctgt
      181 tgaggcagcc cgccgtgctc gttccagtag gtgaggaact tgccgcgcac ggacttgccg
      241 gtctcaggga aggtgcgcga ctcgccctgc aagtgggcgt aagagggcgg gaagagaggt
      301 atggcgacca cggccattac aaaggccagg cataggcgtg ctttcttctg cacggtggaa
      361 actgacatcg gtgtctctcc ttcgattggc gtgtttggac ctggccctgt gtctagctct
      421 ctcccgccca ggggctaccc cggcgcggtc caggcaatcc ggtcagcgcg gtatgtccgt
      481 catcagatat atgcaagtag tagctggtag ggttcccagg gtggaatggc gttgtgtgct
      541 tgctaggcag cccctatcac ttgcatggaa ggaccagaat tcccctctgt catcctgagc
      601 gtagcgaagg atgacagagg ggaattctgg tccttgatac cgcccacatg gcatgccccc
      661 ggtagtaaaa gcaagtccgc accgctgtgg cggcagcttc ggcaggactg aaggcacact
      721 ttgtgcagcc ctactacaac cttgtttatt ggccctaatt ttacttgccg gcacataagg
      781 cgtaacgtgg cgcttatctg acccccagcc aggcgaactc cggggcgcgt gaggcccatg
      841 cttatatggt ctggacgcct gcgttcatgc tctctagtgt cttatgctgc cctgtcacac
      901 atcagggaca atgaagtcaa ccttagtgat gcaaggaagg agcgcagagt tgagaagcag
      961 gatagcattg gaccgccgca agctcatact ggccgtgttg ctactgtgtc tgattgtcac
     1021 ggcagctttc gcggtgcggc tctcccaggt gcagccgggc acggcgctca ccagggcaga
     1081 cgtggctaat agcctctacg tggcggaacc cggggaaagc gggcacgaag agagcatgat
     1141 gttgagcagc agggatgaat tcctcaacgc ccgctacacc tacccgaccg gcaaggccga
     1201 ccgccggtgg gtgtttgagg ccgccaagca agacaagctc ataccctctg gcataccggc
     1261 gggccgggtg acttacaagg gtccctccag ccccaacgcg cccgacgtgc tcaatgcgaa
     1321 ccagtggatc tccataggac cgcagcctca gcagagcgag acctgctcgg tgtgcttccc
     1381 gttcgttgaa gtggccggac gcgtgaacga tatcgtggtt gaccctatca ctcccacgat
     1441 agcttacatc gcttccgacg cgggcggcgt ttggaagacc accaactgct gtcgcgccgc
     1501 tactgcctgg tcgcccacta tggacgaccc gtatatctcc acgctcgcga ttggcgacct
     1561 ggcaatcgag ccgaacggac ataccatcta cgcgggcaca ggcgacctgc gcttcggcac
     1621 cttctcgttc ggcagcggag gtctgctcaa gagcaccgac ctcggcgcta cctggcaggt
     1681 caagggctac gacgtgtttg agcctaagta cccacagaac gcgggtgtct tcccacaata
     1741 caactcgata ggcaaggtag ctatcaaccc gcacgacaac aacgacctgg ccgtgggcac
     1801 caagctcggc atgtacttct cctacgatgc cggggacagc tgggccgggc cttgcttgcc
     1861 cgacccgttc cccaaccagc gccaggacat cacggctatt atcgctatga cccagacgaa
     1921 caccctggcc gacctgttcg tggcggtggg tgcgaggggc tacagcacca ccgtgcagac
     1981 caacctggcg gaaaacggcg ccaacagcat ctacaagacc acctggcctt caagcggctg
     2041 ccccgcaagc tggagccatg taagccgagc cgataacggc tggcctccgg gcacgggcac
     2101 cggcgcaccc gtgtatatga acggcggcaa cctgttgggc cgcatcgaca tggctttctc
     2161 ggaaagcaac cccgactaca tttatgccga ggtacaggcc atcaaacccg gctatggagc
     2221 tatccagaga ggtggaatgc tcggcatctg gcgctctacc aaccgcggag aatcgtggga
     2281 gcagcgcaca accgcccaag acctggaaga cgcgcagtat gcgtgcggtg gcgattgtct
     2341 ggccgattcg ttgctgggtg tgtgcggaga cgtggctcag aactggtacg accagcatat
     2401 agcggtcgat cccaacgatc ccgatgtgat cttcttcgac aacatcaacg tctggaagtc
     2461 taccgatggc ggcgaaacga taaaagacct cacctgcggc tacagctcca tccaggtgcc
     2521 gcgcccggtg cacgtggacc agcacgccat caccttcctg cctgactcat caagccggca
     2581 gctaatcggc aacgacggtg gggtctacgt caccgagaac gccaataccg agcagccggt
     2641 gtacgtgcag ctcaacacca ccctgagcac gattgagttc tacggcggcg acatcagcgc
     2701 caacttcgct accagtcctg cccccttcgc ggtggcaggg gcgcaggaca acggctcctc
     2761 ctcgtggcag ggcgccgctg acgttgcgac ctcgcccagg gtgtggcaac agcgtatcgg
     2821 cggcgacggt atgttcgccc gcatagagcc tgtgcatggc ctgcgcgtct acatggaagc
     2881 ccagggaggg gctatccggc ggtctgacgt cggccaccag gggccctacc aggtggcttg
     2941 cctcaatatc gacggcattc ccaacacgcc gggctgcgtg cccacgaact tccaggtgga
     3001 caacccgcga gtaagcttca tcttccccta cgagatctac acgggcgtac cggcagccaa
     3061 cggtccgggg gacgagtgcg accagacgga gggctgcctg cacttcatcg gcggcaccta
     3121 ccgcgtgtgg gagaacctcg acggtctgcg ggatggttcc ccggcgggca tcccgtggta
     3181 ccctaccagc cccgacctga ccaagaacac cctggccgac cgctcgcaca tcaaccagct
     3241 agccttcgcc ttcaggacca acaagacggc catagcaggc accaacgacg gcaacgtctg
     3301 gatcggcttc ggcctcggcg cggggcaggg tgtgacttcc acctggaaga acctgaccgg
     3361 cggcaacgcg gtcctgccca accgccctat cctggacgtg gtgctcgacc cggccgtgct
     3421 cagcacgacc accaacccgg taggcttcgc ggctgtgggt ggcttcaacg aaaacacccc
     3481 gtctcagccc ggccacgtct tcaaggtgac ctgcggcagc aactgcgaca cgtccacctg
     3541 ggagaacaag agcggcaacc tgcccaacat cccggtggat gccatcacgg tcaaccccaa
     3601 ctatccctcg caggtgttcg cgggcacgga ctggggagtg tactacacga acgacatcaa
     3661 cgcggcatcc cctatgtggt accacttcaa ccagggcatg cccaacgtca tgatctggga
     3721 cttcgcgata gaccggggct tcaccaccct ggcggcgttc acccgcagcc gtggcgcgta
     3781 cgtgtggccc ctgccctctg cccccttcga gcagactcct accgccaccg ttacaggcac
     3841 gccgcccgcc acctccaccg cttcccctac cgcgatgacg cgcactcctg atgtgcctat
     3901 cacctatact cccgtgccac ccacccacac ggctaccccg ttggccacgg cgaccatgct
     3961 ggcttgcggc tcggagcggc tcctgtacga aacgttcgag ctcggcacgc ttggggcctt
     4021 taccgccaag accgagacag acaccggc
//
LOCUS       DASRWZ010000405         5029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_102942, whole genome shotgun sequence.
ACCESSION   DASRWZ010000405 DASRWZ010000000
VERSION     DASRWZ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102942"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2977
                     /locus_tag="VFH60_06270"
     CDS             <1..2977
                     /locus_tag="VFH60_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6313422.1"
                     /translation="IYAGTGDLNFGSFSMGSAGILKSTDAGVTWAIKGADVFTGYYPE
                     PAGTFPQYQAVGKIEADPNNGNTLVAGTKTGVYFSYNGGDTWSGPCLTNSFSSQRQDI
                     TGLVLTDAGSSTDMFVAVGTRGYSTTVQYNLAENGANGIYKSTVPASGCPANWSLLTR
                     PDNGWPAGTGSGIPVYQAGGNTLGRVDIDISNSNPNYIYAIVQAIGTYQGGVKGVWRS
                     IDGGANWEMRAGLGNSATEWTGCARTGQQNWYNQHIEIDPLNPEHVWVSTIDIFKSSN
                     GADTFTNITCGYNGGTTVHVDHHAIEFVPGSSEALLAGSDGGAYVTLNATAGTPAFSR
                     LNDSLSTIEFYGGDITANFANDTNPGANGGSQDNGSMVNVWSAPTPVGPALWQLRKGG
                     DGMFARIEPVQELRWYQESQNGNLAVTTNGPYSSQVNATGGWTADRLSFIFPYEIYKY
                     DCPATGCTHLIAGSYRVWETITGGVGGGSWIPISGDLTKNTLADRSFINQLSYSVSLS
                     TTAIVGTNDGNVQYGFGLGAGAATATWVNVTGGNSVLPNRPILDVTTDPVTPTIGYAA
                     VGGFDQNTPSTPGHVYRVTCTANCASFTWENKSGNLPNIPVDSILANPRYPNQVFAGT
                     DWGLYYTNDITAASPTWFRFQTGMPNVMIWDMSIDMGFTTLSVWTRGRGAYVWPLPEG
                     PFTGGTPTPSVVPTVGTAVPSVTTAPSVTAIPSVTALPSVTVLPSVTAIPTGSAVATG
                     TATPVLQTATAVVSGTPVVATGTSTAVAATSTSTAIATTTATPCTVQFTDVPPDSTFY
                     VFIRCLACQGIMGGYSDGTFRPYNDITRGQLSKIVANSAKFNEPVSGQAFEDVAPNST
                     FYEFIGRMASRGIIGGYPCGGQGEPCGPGNRPYFRPNANATRGQISKIVSEAAGFDDI
                     PEGQTYQDVPPENTFYVWIERLSSRGVMSGYPCGGTGEPCGTTGKPYFRPNNNATRGQ
                     TSKIVANTFFPDCNPTARP"
     gene            complement(3081..3443)
                     /locus_tag="VFH60_06275"
     CDS             complement(3081..3443)
                     /locus_tag="VFH60_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET6313423.1"
                     /translation="MGDQNYSDTCIFCRLAKGLVRADIIFEDDAVIAFNDIHPQAPTH
                     VLVIPRQHITALWETDDSHNALLGRILLVCNRVAQQQGIMDSGYRVVVNTGADAGQSV
                     DHVHFHVLGGRKLQWPPG"
     gene            complement(3621..>5029)
                     /locus_tag="VFH60_06280"
     CDS             complement(3621..>5029)
                     /locus_tag="VFH60_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET6313424.1"
                     /translation="LLQERIPHGGPVELYHALDRQLQRLVTVQMLSAHAADDVEMVRR
                     FRSHQQAAGSLHHPSICEVYDAGEWEGRPYSVMEKDSALPASSLYREDGEPPDMAAAL
                     RVTRQVAEALQHCRDSGLADWAFSYRAVRLDNACNAHLLLVEGLDQAHGNDEYYTSTS
                     VEDDPRALSGLLRVMMVGNPDPRAAGLVMLSLPQPIQELLSRLYLEGEGHITSAGEAA
                     QAIASMEAAHSAPTEAYGPDMVAATAPGDAPTLNLAEQPTMVTVPPPTAYVPDGEITS
                     EPHAPVEADSGEKGRNRLPIILPLLGLLLLAGLVAAFWPRQALSGTPVEEAVAAPAPT
                     TPPPLLAPDLRGKSLPEAQIATHAAGLNLAQADTLYDAAFPKDTIARQQPEAGAQVQP
                     GAVITVNLSLGAEPQVAFPERTPEPQPPIVIDNPTPKPQTDQQPPAKKPSEDKKSPPG
                     KDKDDGDHKGNKEKDKDD"
BASE COUNT         1024 a         1607 c         1493 g          905 t
ORIGIN      
        1 catctacgcg ggcaccggcg acctcaactt cggctcattc tcgatgggca gcgcgggcat
       61 cctcaagagc accgacgcgg gcgtgacctg ggccatcaag ggcgcggacg tgttcacagg
      121 ctactaccct gagcctgccg ggacgttccc gcagtaccag gccgtaggca agatagaggc
      181 cgaccccaac aacggcaaca ccctcgtcgc gggcaccaag acgggcgtct acttctcgta
      241 caacggaggc gacacctggt ccggcccctg cctcaccaac tcgttctcct cacagcgcca
      301 ggacataacc ggcctggtgc tgactgacgc gggcagcagc accgatatgt tcgtggctgt
      361 tggcactcgc ggctacagca cgacggtgca gtacaacctg gccgagaacg gcgcgaacgg
      421 catctacaag tccaccgtgc ccgccagcgg ctgcccggcc aactggagcc ttctcacccg
      481 gcccgacaac ggctggcccg caggcaccgg cagtggcatc cccgtctacc aggcgggcgg
      541 caacacgctg ggccgcgttg acatagatat atcgaacagc aaccccaact acatctacgc
      601 tatcgtgcag gctatcggca cgtaccaggg cggcgtcaag ggcgtgtggc gctcgataga
      661 cggcggcgca aactgggaga tgcgcgccgg cctcggcaac agcgccaccg aatggacggg
      721 ttgcgcccgc accggccagc agaactggta caaccagcat atagagattg acccgctcaa
      781 cccggagcac gtatgggtca gcacgattga catcttcaag tcgtccaacg gagccgacac
      841 cttcaccaac atcacctgcg ggtacaacgg cggcactacg gtccacgtgg accaccacgc
      901 tatcgagttc gtgcccggct cttcggaagc actgctcgca ggtagcgacg gcggcgctta
      961 cgtcaccctc aacgctaccg ccggtacgcc cgccttcagc cgcctcaacg actccctcag
     1021 cacgatagag ttctacggcg gcgacatcac ggccaacttc gccaacgaca ccaaccctgg
     1081 ggccaacggc ggctcacagg acaacggctc tatggtgaac gtttggagcg cgcccacccc
     1141 ggtcggcccg gcgctgtggc agcttcgcaa gggtggcgac ggcatgttcg cccgcatcga
     1201 gcctgtgcag gagcttagat ggtaccagga gagccagaac ggcaacctcg ctgtgaccac
     1261 caacggcccg tacagcagcc aggtaaacgc gaccggcggc tggactgcgg acaggctcag
     1321 cttcatcttc ccctacgaga tatacaagta cgactgcccg gccacaggct gcacccacct
     1381 catcgcaggc agctatcgcg tgtgggagac tatcacgggc ggcgtcggag gcggtagctg
     1441 gatacccatc agcggtgacc tgaccaagaa taccctggcc gaccgctcgt tcatcaacca
     1501 gcttagctac tcggtcagcc tgtcaacgac ggctatcgtc ggcaccaacg acggcaacgt
     1561 gcagtatggc ttcggcctgg gcgcgggtgc ggccaccgcc acgtgggtga acgtcacagg
     1621 cggcaacagc gtgctgccca accgccccat cctcgacgtg acaacagacc cggtaactcc
     1681 tacgatcggc tatgcggcgg tgggcggctt cgaccagaac acgcccagca cgcccggcca
     1741 cgtatacagg gtgacatgca cggccaactg cgcgtcgttc acctgggaga acaaatcggg
     1801 caacctgccc aacatccccg ttgactctat cctcgcgaac ccgcgctatc cgaaccaggt
     1861 attcgcgggc accgactggg gcctctacta caccaacgac atcacggcgg cctccccgac
     1921 gtggttccgc ttccagacag gcatgccaaa cgtgatgatc tgggacatgt ccattgatat
     1981 gggcttcacg accctgtcgg tgtggacgcg tggacggggc gcatacgtat ggcctctgcc
     2041 ggagggaccc ttcaccggcg gcacacccac ccctagcgtc gtgcccacgg ttggcaccgc
     2101 agtacccagc gtcactacgg caccaagcgt caccgcaata cccagtgtca ctgcattacc
     2161 tagcgtcacc gtattaccca gcgttaccgc gatacccacc ggcagcgcag tggcgacagg
     2221 cacggctact cccgtactgc agacggccac cgccgtcgtg tcaggcacgc cggttgtagc
     2281 taccggcacc tccaccgccg tagcagccac ctccaccagc acggcaatag ccacaactac
     2341 agctactcca tgcacggtgc aattcaccga cgtgcctccc gacagcacct tctacgtgtt
     2401 catacgctgc ctggcgtgcc agggcatcat gggcggctac tcggacggca ctttccggcc
     2461 ctacaacgac atcacacgcg ggcagctatc caagatcgtg gcgaactccg cgaagttcaa
     2521 cgagcctgtt agcgggcagg ccttcgagga cgtggcaccc aacagcacct tctacgaatt
     2581 catcggcagg atggcaagca ggggcatcat cggcggctac ccgtgcggtg ggcagggcga
     2641 gccttgcggt ccgggcaacc gcccatactt caggcccaac gccaatgcta ccaggggaca
     2701 aatctccaag attgtctcgg aggcggcagg tttcgatgac ataccagaag gccaaaccta
     2761 ccaggacgtg ccacccgaaa ataccttcta cgtgtggata gagcgtctat cttcaagagg
     2821 ggtgatgagt ggctacccat gcggaggcac cggcgaacca tgtggcacca ccggtaagcc
     2881 atacttccgc ccgaataaca acgctaccag ggggcagacg tcgaagatag tggctaacac
     2941 tttcttcccc gactgcaacc cgacagccag accatagacc aataaagggt gaaagcaaaa
     3001 tggcaggcgc gatgcctgcc attttgcttt gctccgcgaa aagagaatgc tccgaagggc
     3061 aaaagcaaaa acctctgccg tcaaccgggc ggccattgaa gcttgcgccc gccgaggacg
     3121 tggaagtgaa cgtggtccac ggactggccc gcgtccgcac cggtgtttac caccacgcgg
     3181 tagccgctat ccatgatgcc ttgctgttgg gcgacgcggt tgcagacgag caggatgcgg
     3241 ccaagcagag cgttgtggct atcgtccgtc tcccacaggg cagtgatatg ctggcgcggg
     3301 atgaccagta cgtgcgtggg tgcctggggg tggatgtcat tgaaggctat caccgcatca
     3361 tcctcgaaga tgatgtccgc ccgcaccaac cctttcgcca gcctgcaaaa tatgcaagta
     3421 tctgagtaat tctgatctcc catattacct ccttgcgtga aacgtaaaac gtattgcgta
     3481 ttgcgtattg cgtaattatt gaaccacaaa gacacaaaga aacacaaagt tcgctaagat
     3541 aaccagaata aaaccgcaga gacgcagagg ctatttagag ttctacctga tacgcaatac
     3601 gttttacgtt ttacgtttct ctagtcgtct ttgtccttct ccttgttgcc cttgtggtca
     3661 ccatcatctt tgtctttccc tggtggggac ttcttatctt cagatggctt cttcgcgggt
     3721 ggttgctggt cggtctgggg cttgggtgtg gggttgtcta tgacgatggg cggctggggt
     3781 tcgggcgtgc gctcagggaa ggctacctga ggctccgcac ccaggctgag gttgacggtg
     3841 atgacagcgc cgggctgaac ctgggcaccc gcttccggct gctggcgggc gattgtatcc
     3901 tttgggaaag cagcgtcgta gagggtatct gcctgggcaa gattcagtcc ggcggcgtgc
     3961 gttgcgattt gcgcttcggg caggctcttg ccccgcaggt cgggcgcgag cagtgggggc
     4021 ggcgtggttg gggctggggc cgcaaccgct tcctctacag gggtccctga gagtgcttgc
     4081 ctgggccaga acgcagccac cagaccggcc agcaacagca acccaagcag tgggaggatg
     4141 ataggcaggc ggttgcgccc tttctcgccg ctatctgctt ctacgggggc atgcggctcg
     4201 gaagtaatct caccatcagg cacgtatgcg gtggggggtg ggaccgtgac catagtaggc
     4261 tgctcggcga ggttcagcgt aggcgcgtca ccgggagcgg ttgcggcgac catatcaggg
     4321 ccgtatgcct cggtgggcgc gctatgtgcc gcttccattg atgctatggc ttgcgcggct
     4381 tcccccgcgc tcgtgatgtg gccctctcct tcgagataaa gcctgctcag caattcttgt
     4441 atcggctgcg gcaggctcaa catcaccagc ccagcagccc tggggtcggg gttgccgacc
     4501 atcatgacgc gtagcaggcc gctcaaagcg cgcgggtcgt cttcgacgct cgtggaagta
     4561 taatactcat cgttgccatg ggcctggtct agaccttcca ccagcaggag gtgggcgtta
     4621 catgcgttgt cgaggcgcac ggccctatat gaaaaggccc aatccgccag cccggagtcg
     4681 cggcagtgtt gcaaagcttc cgccacctgg cgcgtaactc gtaacgcggc tgccatatcg
     4741 ggcggctcgc catcctcgcg gtagagagag gaggcgggca gcgcgctatc tttctccatg
     4801 acggagtagg gccgtccctc ccactctccc gcgtcataca cttcacatat ggacgggtgg
     4861 tgcaagctgc ctgccgcctg ttggtgcgag cggaagcgcc ggaccatctc aacgtcgtct
     4921 gcggcatgcg ccgaaagcat ctgcacagtg accaatcgtt gaagctggcg gtcgagggca
     4981 tggtaaagct caacaggccc accgtgtggg atgcgctcct gcaatagat
//
LOCUS       DASRWZ010000406         8642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_171707, whole genome shotgun sequence.
ACCESSION   DASRWZ010000406 DASRWZ010000000
VERSION     DASRWZ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171707"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VFH60_06285"
     CDS             <1..473
                     /locus_tag="VFH60_06285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313425.1"
                     /translation="RVQFLMLGEGPRKAALLEQCNALGLTNLRMLPEVATTSMPAYLS
                     MADCAIVPLKDEPVFYGALPSKMFEAWACGTAVALSVAGEAVDVLREAGGGVAVPPED
                     PQALARAIAELRARPERTREMGLMGQQYVRERYTRQEQARRLEALLREVKRGTR"
     gene            complement(551..760)
                     /locus_tag="VFH60_06290"
     CDS             complement(551..760)
                     /locus_tag="VFH60_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313426.1"
                     /translation="MAEILSDNTYNLLAILLSKLEGLEAYDTYLQDANDDSRKIIEQI
                     ARDDERHAEMLRSAVESLVKNGGLK"
     gene            925..1827
                     /locus_tag="VFH60_06295"
     CDS             925..1827
                     /locus_tag="VFH60_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6313427.1"
                     /translation="MELSPQMETRSASVYDTHVHYTARGPQDARFVYVGINGLMGGGD
                     SFWPVIQGVPQDWRVVLPDLPGCGESKPLPPPYKHDIAGYARWLAEFLRALGLADRHV
                     VLASVATGAPISVRYALDNKDRVAGQVLHLPFLGKVAIAAKWARPIVAYGLRVGPLRS
                     LVDNLRASDWLMHKIILHEPPDAIPELAERDIDHKQQADLAAAGELLHNLMLTDARTE
                     LPSISSPLLILASEHDFSAPLPVLEGIIAGHPERHMYVYHGGQHSWNEEFIDEMNREI
                     AQFCAQVEAETAPTLPSETASDLG"
     gene            1903..1975
                     /locus_tag="VFH60_06300"
     tRNA            1903..1975
                     /locus_tag="VFH60_06300"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1936..1938,aa:Lys,seq:ttt)
     gene            2201..4783
                     /locus_tag="VFH60_06305"
     CDS             2201..4783
                     /locus_tag="VFH60_06305"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET6313428.1"
                     /translation="MSNLDIPKARPPIQPWLPADEAVWDSPDEAGPPLLPEPGPSADL
                     PTTAVPRRWTLLVFAITLVVYLVLIPRFLLYSSPPTGDQPFYMMDTISLLKDGDLILN
                     NNYADRDERIFYELAPHHEGFVGMSAPYPLPPQLGVTPAKPEEEWYAAHLPGLSVLII
                     PAWLIGELFQLWWPATVVFMCILGALLATNVFLLGTEITGKRWIGLAVWLPLAFSTPL
                     MSYSYLLFTELPVGLLMVYALRRLALGWSTNGPWRLLLIGFCIGYIPWLAFRCAPIAG
                     VLGIYALVQWWRAWRQRPAAGKDEPTETDGVKWPSRPPPRFVWQLGVLLLPVAIAGVV
                     LALFNLNLHGTILPAGGGSITRGQESLFHWPWRGQEELAKWLNGVFGTFIDRNMGLFT
                     NAPIYVLSLVGIVAMFRSGLSSARRLLILMAFLVVPYMTVISAYEWWNGVWCPPARYW
                     NTFVPLLAALLAASLAALANSWFYKALYVVLALPGFYFQAITMYDARHMWPSQAILGR
                     PPSEWPFNLNVRDFVPLFTPIDEYVHPSATAQVVAVAVAIAFIGYILLVRRRGVPRLP
                     FMAHGAIWAVSLMTVGAGWYSMNLDYLQHKTQLVEQHRWALDPVPRLPRGIGYLDGKV
                     YITDYDGNAVGQLDTATGAYKLVEPRKDGGPLPFKRPGDVKVGADGLLYVLNNDQQPH
                     TMYVMQPDGTVVKELVLAGKSEVAVGLYFTPDGTLYLADMRGAQVRKYTSDGSGPVSG
                     TGGETGGFNNVVGVVVEPDGTIYAAESSANRVQRLDAEGKFVRDYDLPCQPMYLVSAG
                     DWLEVSCGQSIFSINKATGVVQRSQTAPDTPRLQNPLGMTYGPDGILYIVDRDAVVAY
                     RVEH"
     gene            5107..>8642
                     /locus_tag="VFH60_06310"
     CDS             5107..>8642
                     /locus_tag="VFH60_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6313429.1"
                     /translation="MQEVEENGPPVHEALDQPASTTAEVPPPAAAATLPDVAPPTEAT
                     LATQAEAGGPSKPEAPRATPTDPDDLGARFEEGLRDWILDTAKGIVTSTATVLFVLLL
                     VQLGLASEGQDLSFKLFSPYEFWKSFSGLVVQWSQWNEWLLLALALLTLAVTVGLFAW
                     WRRHQARETKHHFPGHDAVSASITARKSKRRNLYFRVQSSLRSMKGVSAFGMISAILF
                     GAYFYQQYLWNVELPVPEGQIGIAFTRQVGNSIARDTLAESLRLRQLGHGDEIVLRDL
                     PVSFDPKDTSQAQRFARRIHAHAVIIYNEVKVTSGGGTVGLRVPGVASPARQATDDRR
                     FVAYLVFADPSLGIEIPVTQEAGAGQPERLSVRTKDGLEAPRLEGTDTTRLMEAAAGV
                     LLYDRDRYPAAVAHFRNAVGNGGLGSSSDALIYLYLGNAYYLLNQDDDAVEAFDSAIR
                     AGESIKQPGVQERLVLAQAYISRAQLYFYSYDTEAAETLLRKALRLKETLDQDETALS
                     NPAIFRQLHETAGNAYSRLLDIALLNKDEDAKQLWLTRVGDEARALATRPDDSRSMAA
                     AARFLYRSGACEEAYGIAYGQLDKDPRNANLHRTVASLAVLRDQSLMSLESVQQRKEL
                     LKLNPASLPDLHYMLLRYSLNATSSDIGYLDKVKETADAILAVDPTNVQAIERYLTTV
                     DVAGTYDLLLLLVDPENMESLADFSPYLEGAGGVAALMDPAYGYSPIGDTRTLRKVLT
                     EQLQDPETIKRMLAIKEGARPYIVRWAQEADPDTAEPIIFAARYSRNLNSYVSLYSSM
                     GGVMDQEVLDRVWQRAFDDATAALESDRRPSPRQLAEAHTSLSSLYRSSYLMKFLQQD
                     KEGAGEMLNLALDHSRTAADLIDSNPPESPTPGELSLDYTVYNDYSLAAYFATFYYDW
                     VGDQAKIDEYTKLHETLAPKATELQAQANKPLGQNYDYLRSLTCNGDNLLKQGTDALA
                     AGELDRAVELLTGYTGQYSYDPLGAWSLAWAQYRNGDPAAALQTLDRASEFSGDVATL
                     WGKKVVVLLAQDKSDEAAHSLTEFYTRLESEPVEVRVRELVGMGRDLLELARDDYDAR
                     AGIKALLPEVERYVDSLPAEARKAEGAQMILALDSLGSASTWAGDYDRARKFLQAAVE
                     INGDYLPARANLALALLASGDAKSAGSEYDRAV"
BASE COUNT         1643 a         2867 c         2584 g         1548 t
ORIGIN      
        1 cgcgagtcca attcctcatg ctgggtgagg ggccaaggaa ggcggcttta ctggaacagt
       61 gtaacgcgct gggcctcacc aacctgagga tgctgccgga ggtggcgacc acctccatgc
      121 ccgcctacct ttcgatggcc gactgcgcta tcgtgccgct gaaggacgag ccggtctttt
      181 acggcgcttt gccctccaag atgtttgaag cctgggcttg cggcacggcg gtcgcgctga
      241 gcgtggcggg cgaggcggtg gacgtgttgc gcgaggccgg gggaggggtc gcggtgccgc
      301 cggaagaccc gcaagccctc gcccgtgcga ttgctgagtt gcgggcgcgg cctgagcgga
      361 cgcgggaaat gggcctgatg ggacagcagt acgtgagaga acgctacacc cggcaggaac
      421 aggcgcgcag gctggaggcg cttttgcggg aagtcaaacg cggcacccgc taggaggacg
      481 ccgcgtctgt gctcgtatct attacccggc ctcacattca gagtagggcc aggctatcta
      541 cggctcggac ctacttcagc ccgccgttct tcaccagcga ctcgactgcc gagcgcagca
      601 tttcggcgtg gcgctcgtcg tcgcgagcga tctgctcgat gatcttccgg ctgtcgtcgt
      661 tggcgtcctg caagtaggtg tcgtaggctt cgaggccctc aagcttgctc aacagaatgg
      721 caagcaggtt gtacgtgttg tcacttagaa tttccgccat tattgtcctc ctggattgat
      781 tactggctta atcgccacga cttgaggagc aagttgcgtg cccgtgtgga cgcccgaaca
      841 ctggaacgca caacggcaca gcgattgcaa cacccctccc aataccatgc aataccgcga
      901 tacgcacgca ggaggctaca cagcttggaa ctaagcccgc aaatggaaac ccgctcggca
      961 agcgtctacg acacgcacgt acattacacc gccagggggc cgcaagatgc acgttttgtc
     1021 tacgtcggca tcaacggcct catgggcggc ggcgactcgt tctggccggt catccaggga
     1081 gtgccccagg actggcgcgt ggtgctgccc gacctgcccg gctgcggcga gtcgaagccc
     1141 ctgcccccac cttacaagca cgacatagcc ggttacgcgc gctggctagc cgagttcctg
     1201 cgagcgctgg ggctggctga caggcatgtg gtgctcgcca gtgtagctac gggtgcgccc
     1261 atctcggtgc gatacgcgct ggacaacaag gacagggtag ccggacaggt gctccacctg
     1321 ccattcctgg gcaaagtcgc gattgcggcc aagtgggcca ggcccattgt ggcctacggc
     1381 ctgcgggtcg gccccctccg ctccctggta gacaacctgc gcgccagcga ctggctcatg
     1441 cacaagataa tcctgcacga gccgcccgac gctatcccgg agcttgccga gcgcgatata
     1501 gaccacaagc agcaggccga cctcgcggcc gcgggcgagc tactgcacaa cctgatgctc
     1561 accgacgcgc gcaccgagct accctccata agcagccccc tccttatcct cgcctcggag
     1621 cacgacttct ccgcgcccct gcccgtactc gaaggcatta tagcgggtca cccggagcgg
     1681 cacatgtacg tgtaccacgg cgggcagcac tcctggaacg aggagttcat agacgagatg
     1741 aaccgcgaaa tcgcccagtt ctgcgcgcag gtcgaagctg aaaccgcgcc cacactgccc
     1801 tccgagacgg catccgacct tggttaggcg catctggttt ctggtataat ggtacgttgc
     1861 cacgtcgcgt cccgcactgg acgagtgggc taacgtcgaa atgtgcccgt agctcagtcg
     1921 gtagagcatg cgacttttaa tcgtagggtc ccgggttcga gtcccggcgg gcacaccccg
     1981 aaacgtattt ccacggggga tctccgtacc tgtatggctg ggtgaatcgg cggtgcgagg
     2041 tatccccacg cacccgcagt agttctcttg acacacgtgt ataattatga accggcaggc
     2101 tgtcaagact atcaactcat caaaaactca ggagtaccga ggcttgatag cacctgcttc
     2161 aacgaccgcg aacgcactaa agtaagctac ctcagcatac atgtctaacc tcgacatacc
     2221 caaagctcgt ccgcccatac aaccctggct ccccgcagat gaggccgtct gggactcacc
     2281 cgatgaagcc ggccctcccc tgctgccgga gccgggtcct tccgccgacc tgccgaccac
     2341 cgccgtgccc aggcgctgga ccctgctcgt gttcgcgatc accctggttg tctatctcgt
     2401 cctcattccc cgcttcttgc tctacagcag ccctcccacc ggcgaccagc cgttctacat
     2461 gatggacacc atcagcctgc tgaaagatgg cgacctgatt ctaaacaaca actacgccga
     2521 ccgggatgag cgtatattct acgagctagc ccctcaccac gagggcttcg tgggcatgag
     2581 cgcgccatac ccgttgcccc cacaattagg cgtcaccccc gcgaagcccg aggaagaatg
     2641 gtacgccgcc cacctgcccg gcctgtcggt gctcatcata cccgcctggt tgattggtga
     2701 gttgttccag ttgtggtggc cggccaccgt ggtctttatg tgcatactgg gtgccctgct
     2761 tgccacgaac gtctttctcc tgggcacgga aatcaccggc aagcggtgga tagggttggc
     2821 cgtgtggctg cccctcgcgt tcagcacccc gctgatgagc tactcctacc tcctcttcac
     2881 cgaactgcct gtgggcctcc tgatggtgta cgctctccgc cggctggccc tcggctggtc
     2941 taccaacggc ccctggcgtc tgctcctcat cggcttctgt ataggctaca ttccctggct
     3001 ggcattccgt tgcgcgccaa tcgcaggcgt cctgggcatc tatgccctcg tacagtggtg
     3061 gagggcgtgg cggcagcgtc cggccgctgg caaggacgaa ccaacggaga ctgacggggt
     3121 taagtggccc tcgcgtccgc ctccgcgctt cgtgtggcag cttggggtgc tcctcctgcc
     3181 ggttgcaata gccggtgtgg tattggcgct cttcaacttg aatttgcacg gcacgatttt
     3241 accggccgga ggcgggagca taacgcgcgg ccaggagtcg ctcttccact ggccgtggcg
     3301 cggacaagag gaactggcga aatggctgaa cggcgtgttc ggcaccttca tcgaccgcaa
     3361 catgggcctg tttaccaacg cgcctatcta cgtcctgtcg ctcgttggca tcgtcgccat
     3421 gttcaggtcg gggctaagct ctgcacgccg tctgcttatc ttgatggcct tcctggtagt
     3481 gccctatatg actgtaatca gcgcgtacga gtggtggaac ggagtgtggt gcccgcccgc
     3541 ccgctactgg aatactttcg tgcccctgct cgcggccctg cttgccgcct ccctggccgc
     3601 cctcgcgaac agttggttct acaaggccct ctacgtggtc ctggccctcc ccggcttcta
     3661 cttccaggcc atcacgatgt acgacgcgcg gcacatgtgg cccagccagg ctatcctggg
     3721 ccggcccccc tccgagtggc ccttcaatct gaacgtgcgc gatttcgtgc ccctcttcac
     3781 gccaatcgat gagtacgtgc acccaagcgc aaccgcccag gtggtcgcgg tggcggtagc
     3841 catcgcgttc ataggctaca tcctgctcgt gcgccgtagg ggcgtccctc gattgccgtt
     3901 catggcgcat ggtgccattt gggcggtaag cctgatgaca gtcggcgccg ggtggtactc
     3961 tatgaacctc gattatctgc aacacaagac ccaactggtc gagcaacata gatgggcgtt
     4021 ggaccctgtt cctagactgc cgcgcggtat cggttacctc gatggcaagg tctacatcac
     4081 cgactacgac ggcaacgcgg ttggtcagtt ggatacggcg accggcgctt acaagcttgt
     4141 ggagccacgc aaagacggcg gccccctccc gttcaaacgc cccggcgacg tgaaggttgg
     4201 cgcggacggc ctgctctacg tgctgaacaa cgaccagcag cctcacacga tgtatgtaat
     4261 gcagccggac ggcaccgttg tcaaagagtt ggtccttgcg ggcaagtcgg aggtggccgt
     4321 agggctgtac ttcacgccgg acggcacgtt gtatttggcc gatatgaggg gggcgcaggt
     4381 acgcaagtac accagcgacg gcagcggccc cgtatccggc actggaggcg agaccggggg
     4441 attcaacaat gtcgtgggcg tagttgtaga gccggacggc acgatttacg ctgccgaaag
     4501 ctccgccaac cgagtgcagc gcctggacgc cgagggcaag ttcgtgcgcg actacgacct
     4561 cccttgccag cctatgtacc tggtctcggc aggcgactgg ttggaagtga gctgcgggca
     4621 gagcatcttc tcgatcaaca aagccaccgg agtcgtgcaa cggtcgcaga ccgctccgga
     4681 caccccacgc ttgcagaacc cgctaggcat gacatacgga cctgacggca tactgtacat
     4741 agttgaccgg gacgcagtgg tagcataccg ggtcgagcat tagggcctat ctgcaaagtg
     4801 taggaagcga gcgaacacag gtggtcttgt cattcttcgc tgcgctcaga atgacaaggt
     4861 cgggatttgg tcccgcacgg gcggcactct gcagactggg cctggaggcc acctcctacg
     4921 tttgccttag ccgtgtcgca cagccttccg gtactacctt agtagcattg gcattgggcc
     4981 tggggcctga cgacaagccg agccacccca catataatcg tcagaaaaac gtacaggcag
     5041 accagacaag catccaatta agtagcgcgt atataaaaga agcatgcaag aaggactaac
     5101 aaaccgatgc aggaagttga agagaatggc cctccggtac acgaggcgct agaccagcct
     5161 gcttcgacaa ccgccgaagt acccccgccg gcagcggctg ccaccttgcc cgacgttgct
     5221 ccccccaccg aggccactct agctacccag gccgaagccg gaggcccctc caagccggaa
     5281 gcccccaggg caacacccac agaccccgac gacctgggcg cgcgctttga ggaagggctg
     5341 cgcgactgga tactcgacac tgccaagggc atcgtcacct ctacggccac ggtgctgttc
     5401 gtgctcttgc tggtgcagct tggcctggcg agcgagggcc aggacctctc cttcaagctc
     5461 ttttctcctt acgagttctg gaagagcttc agcggcctgg tcgtccagtg gtcgcagtgg
     5521 aacgaatggc tgttactggc gctggcgctg ctcacgttgg ccgtgacggt tggcctgttc
     5581 gcctggtggc ggcgtcacca ggcacgtgaa accaaacacc attttccggg gcacgacgca
     5641 gtctcggcct ccatcactgc gcgcaagagc aagcggcgca acctctactt ccgcgtgcag
     5701 tcgtccctcc gtagcatgaa gggcgtgagt gcgttcggca tgattagcgc catcctcttc
     5761 ggcgcgtact tctaccagca gtacctctgg aacgtcgagc tacccgtccc cgaaggccag
     5821 atcggcatcg cgttcacccg gcaggtcggc aacagcatcg cccgcgacac cctggccgag
     5881 agcttgaggc tgcgtcagct ggggcacggc gacgagatcg tgctgcgcga cctgcccgtg
     5941 tcgttcgacc ccaaagacac ctcccaggcg caacgcttcg cccggcgcat ccacgcgcat
     6001 gccgtaatta tctacaacga ggtgaaggtc acttccggcg ggggcaccgt cggcttgcga
     6061 gtgcccggcg tggcctcgcc cgcccggcaa gccaccgacg accgccggtt cgtggcctac
     6121 ctcgtgttcg ccgaccccag cctcggcatc gaaatccccg tgacgcaaga ggccggggcg
     6181 ggccagcccg agcggctgtc ggtgcgcacc aaggacggcc tggaagcgcc ccgcctggaa
     6241 ggcaccgaca ccaccaggct catggaagcg gctgcgggcg tgctcctgta cgaccgggac
     6301 cgctaccccg ccgccgtggc ccactttcgg aacgccgtcg gaaacggcgg gctgggtagc
     6361 tcgagcgacg ccctcatcta cctgtacctg ggcaacgcct actacctgct gaaccaggac
     6421 gacgatgcgg tggaggcatt cgacagcgcc atacgcgcgg gcgagagcat aaagcagccc
     6481 ggcgtgcagg aacgcctcgt gctggctcag gcgtacatca gccgggcgca gctttacttc
     6541 tactcctacg ataccgaggc cgccgagacg ttgctccgga aagccctccg gctgaaagag
     6601 acgttggacc aggacgagac ggccctctcg aaccccgcca ttttccggca gttgcacgaa
     6661 acggcgggca acgcttactc gcgcttgctc gacatcgcct tgctcaacaa ggacgaggac
     6721 gcgaagcaac tatggctgac tcgcgtaggc gacgaggcgc gcgccctggc taccaggccc
     6781 gacgacagcc gctctatggc cgccgctgcc cgcttcctct accgctcagg cgcgtgcgaa
     6841 gaagcctacg gcatcgccta cggccagttg gacaaagacc cccgcaacgc caacctgcac
     6901 cgcaccgtcg ccagcctcgc cgtgctgcgc gaccagagcc tgatgagcct ggaatcggtc
     6961 caacagcgta aggaactgct caagctcaac cctgccagcc tgcccgactt gcactacatg
     7021 ctgctcaggt actcgctgaa cgccacctcg tccgacatag gctacctcga caaggtcaag
     7081 gagaccgccg atgctatcct ggccgtggac ccgactaacg tacaggccat cgagcgttac
     7141 ctgacgaccg tggacgtggc gggcacgtac gatctgctgc tcctgctcgt tgaccctgag
     7201 aacatggagt cgctcgcaga cttctccccc tacctggagg gtgctggggg cgtggccgcc
     7261 ctgatggacc cggcctacgg ctactcgcca atcggtgata cccgcacgtt acgcaaggtg
     7321 ctcaccgagc agttgcaaga ccccgaaacc atcaagcgca tgctcgccat aaaagagggc
     7381 gcgcggccct acatcgtccg ctgggcgcaa gaggccgacc ccgacaccgc cgagcctatc
     7441 atattcgccg cccgctacag ccgcaacctc aatagctacg tgagcctcta ttccagcatg
     7501 ggcggcgtga tggaccagga agtgctcgac cgcgtctggc agcgcgcctt tgatgacgcc
     7561 acggcggcgc tcgagtccga ccgccgccct tcccctcgcc agcttgccga ggcccacact
     7621 agcctgtcga gcctttaccg cagcagctac ctgatgaagt tcctccaaca ggacaaggag
     7681 ggcgcgggcg agatgctgaa cctggccctg gaccattcgc gcacggcagc cgacctcatc
     7741 gacagcaacc cgcccgagtc gcccacgccc ggcgagctaa gcctcgacta tacggtctac
     7801 aacgactact cgcttgcggc ctacttcgcc accttctact acgactgggt gggcgatcag
     7861 gccaaaattg acgaatatac gaagctccac gagacgctcg cgccgaaagc caccgagttg
     7921 caggcccagg cgaacaagcc tcttggccag aactacgact atctcaggag cctcacctgc
     7981 aacggcgata acctcctcaa gcaaggcacc gacgcgctag ccgcaggcga attggaccgc
     8041 gcggtcgaac tgctgaccgg ctacaccggc cagtactcgt acgacccgtt gggtgcctgg
     8101 tccctcgcgt gggcgcagta ccgcaacggc gacccggcag ccgccctaca gacgttagac
     8161 agagcgagcg agttctcagg cgacgttgcc accctctggg gcaagaaggt agtcgtgctg
     8221 ctcgcgcagg acaagagcga cgaggccgcc cactcgctca ccgagttcta cacccggctt
     8281 gaaagcgagc cggtcgaagt gcgggtgcgg gagcttgtag gcatgggccg cgatctgctg
     8341 gaactcgccc gcgacgacta tgatgcacgc gccggcataa aggccctgct cccggaggta
     8401 gagcgttacg tcgatagtct gcccgccgag gcacgcaagg cggagggcgc acagatgatt
     8461 ctagccctcg atagccttgg ttccgcgtca acgtgggcgg gcgattacga ccgcgcgcgc
     8521 aagttcctcc aggccgccgt ggagataaac ggcgattact tgcccgcacg agccaacctg
     8581 gccctcgccc tcctggcctc cggcgacgcc aaaagcgcgg gcagcgaata cgaccgggcc
     8641 gt
//
LOCUS       DASRWZ010000407         3244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1199772, whole genome shotgun sequence.
ACCESSION   DASRWZ010000407 DASRWZ010000000
VERSION     DASRWZ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199772"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..585
                     /locus_tag="VFH60_06315"
     CDS             70..585
                     /locus_tag="VFH60_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6313430.1"
                     /translation="MLHYKYCPVCATGLQELQLPSEHRPRLVCSGCGHIFYLNPRVVS
                     GTLPVEDGKVWLLRRGIEPRAGYWTHPAGYQELDESTEEAAIRETLEEIGCEVALHRL
                     LGVYSRPHAPVVNVIYLATIADPEQRPQLTPEAVEVRLFGPSDIPWADLAFISTAQAL
                     RDWVSSLPGTE"
     gene            complement(618..1133)
                     /gene="tatB"
                     /locus_tag="VFH60_06320"
     CDS             complement(618..1133)
                     /gene="tatB"
                     /locus_tag="VFH60_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HET6313431.1"
                     /translation="MEIFGMGFGEILLIALVALIVLGPERLPEAARTVGKGISEVRRA
                     VEPARSVWRDLSSELNNVATTTTTAFNAPLAPRRNGAPETIHPVLEMMTEQEKAEFVA
                     GGDMPPRIAEHLAAEAATERPAGSGGTDQAAVELPHLDYAMPHAEAPYRPEAVSEELF
                     YPAPGEAGDRT"
     gene            complement(1176..1541)
                     /gene="cutA"
                     /locus_tag="VFH60_06325"
     CDS             complement(1176..1541)
                     /gene="cutA"
                     /locus_tag="VFH60_06325"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HET6313432.1"
                     /translation="MEARAQTDDPLVMLYVPCGGETEAKGLARRLLDERLIACANLYA
                     SRSLYRWKGETADEQEQVLVCKTLSSRAEAVAARIQQLHSYDIPCILTVTPVRANSEF
                     YRWACGEVAEVVQVDASQS"
     gene            1728..>3244
                     /locus_tag="VFH60_06330"
     CDS             1728..>3244
                     /locus_tag="VFH60_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313433.1"
                     /translation="MSKRPQPSLNAETWSYTLDNALRAAGERILWRRALRACAISLPL
                     LVGVIAFYIALLRFTLADLPHWPVLLVFVPWLLFVWLWTRAQRVTSAEAARFLDRHLG
                     LDERVSTCVEVLNRTRLDRAPGANRGVQFALLVDAAEALDERLLALPGRLSFTWTRWN
                     VIAVSVALVALLAAIAFRTYVELARVERGAVARAVQDQLAQVVALRAEIADNPHLSSS
                     LKAAMLAELDALEQKLQSPNLDQATGIAMLADAEQKLRTRLHTPSADFDGLVAAAQLV
                     WTSAAQNSEWDPEQALSTTDLGRASEASLFLSDNVGGMNNNEERRVSFSLDRASDQAS
                     GRDAALSKLLAEASEGVRTRDHAPAARALVEASQKFAEANNQREDAVALENVLAKLNK
                     GREQIAQAGRPQTKKAQVGFRRRGSAAGSNTPTPDLEDFQPTADAQQPGQAGARGNSS
                     TSGLGPVVGESNVASYGASQSSKDTGGQPAKTGGEDGGGPPGSGPGGGSDGQDSG"
BASE COUNT          573 a         1045 c         1061 g          565 t
ORIGIN      
        1 gaaactgttt gacaactgcc tgcaacgggg gaataatagc ctcagattac gcctgaaatg
       61 tgagcttgaa tgctacacta caagtattgc ccggtgtgcg ctaccgggct gcaagaattg
      121 caactaccca gcgaacaccg accccgcctg gtgtgctccg gctgcggcca catcttctac
      181 ctcaacccca gggtggtgag cggcacgctg ccggtcgagg acgggaaggt gtggctgctg
      241 cgccgtggta tcgaaccgag ggcgggttac tggacccacc cggcaggcta ccaggagctg
      301 gacgagagca cggaggaggc ggcaattcgc gagacgctgg aagaaatagg ctgcgaggtc
      361 gccctgcacc ggttgctggg cgtctattct cgcccgcacg cccccgtggt gaacgtcatc
      421 tacctggcga cgattgcgga cccggagcag cgccctcaac tcacgcccga agctgtggaa
      481 gtgcgcctct ttgggccgtc ggacatccca tgggccgacc ttgccttcat cagcacggca
      541 caggcgctcc gagactgggt ctcctcgctc cctggcaccg aatagcgaga cacgagcgca
      601 gcccgccctt cgccgcctta cgtcctgtca ccagcctcgc ctggcgctgg gtagaaaagc
      661 tcctcagaca ccgcttcggg acggtagggc gcttctgcgt ggggcatcgc gtaatcgagg
      721 tgcggcaact ccacagcagc ctggtccgtc ccgccgctac ccgcagggcg ctcggtcgcc
      781 gcctcagccg ccaggtgttc ggcgatgcga ggcggcatat cccctccagc tacgaactcc
      841 gccttttcct gttcggtcat catctccagg accgggtgta tcgtttccgg agcgccgttc
      901 ctgcggggcg cgagcggtgc gttgaaggca gtggttgtgg tcgtggcgac gttgttcagt
      961 tcgctggaga ggtcgcgcca gacgctacga gccggttcca cagcgcgccg cacctccgaa
     1021 atacccttgc ccacggtgcg agcggcttcc ggcaggcgct caggtcccag cacgatcaag
     1081 gcgaccaggg ctatcagaag tatctcaccg aaccccatgc cgaatatttc cacggtttct
     1141 cctgggtcgc cccatagcgc cggcggccct tcggcctaac tctggctggc gtctacctgc
     1201 acaacctcgg ccacttcgcc gcacgcccag cggtaaaatt cgctgttggc ccgcacgggt
     1261 gtcacggtca ggatgcaggg tatgtcgtag ctgtgaagct gctgaatgcg tgccgccacc
     1321 gcctccgcgc gcgacgaaag ggtcttacag accagtactt gctcttgctc gtcggctgtc
     1381 tcgcctttcc agcgatagag cgaccgggag gcgtagaggt tggcgcaggc gatcagccgc
     1441 tcgtccagca accgccttgc cagccctttc gcctcggtct caccgccgca aggcacgtaa
     1501 agcatcacca gcgggtcgtc ggtttgggcg cgtgcttcca tgtgtgacct ccattataac
     1561 caagcacagg tcccgcaaca acaggcacat ttcgcgcgtg ggacagggca atcttgcgat
     1621 gggttgaact ggcgagtgcc gggcgctgta ctttataatg gaggcgcgta cctctacaac
     1681 tacgtgtcgt cgcctgaact cctggaagtg aggacgcctt gctactattg tctaagcggc
     1741 cccaaccctc tctcaacgct gagacttgga gctacacact cgataacgcg ctacgcgccg
     1801 caggggagcg catactgtgg cgtcgggcgt tgcgggcgtg tgccatctct ctgccactcc
     1861 tggtcggtgt aatagctttc tacatcgcgc tcctgcgatt taccctggcc gacctgccgc
     1921 actggccggt cttgctcgtc ttcgtgccgt ggctcctctt tgtctggctc tggacgcgcg
     1981 cccaacgtgt gaccagcgcg gaggccgccc gattcctcga ccgccacctg ggccttgacg
     2041 aacgtgtctc cacctgcgtt gaggtgctaa atcgcaccag gctggaccgc gcgccaggag
     2101 caaatcgagg cgtgcagttc gccttgctcg tggatgctgc cgaggcgctt gacgagcggc
     2161 tgctagcgct gcctgggcgg cttagtttca cgtggacgcg ctggaacgta atcgcggtgt
     2221 cggtggcgct ggtcgctctg cttgcggcca tcgcgtttcg cacgtatgtg gagcttgctc
     2281 gggtggagcg gggcgcggta gcgcgtgccg tgcaggacca gttggcgcag gtggtggccc
     2341 tgcgcgccga aatcgcggac aacccgcatt tgtcgagcag tctgaaagct gccatgctgg
     2401 cggagcttga cgccctggag cagaagctac aaagccccaa cctggaccag gcgacgggaa
     2461 tcgcgatgct ggcggacgcc gagcaaaagt tgcgcacgcg cctccacacg ccctcggccg
     2521 acttcgacgg actggtggcg gcggcacagc tagtctggac ctccgcggcg cagaacagcg
     2581 agtgggaccc ggagcaggcc ctatcgacca ccgacctggg gcgcgcctca gaggcttcgt
     2641 tgttcctgag cgacaacgtg gggggtatga acaacaacga agagcggcgc gtgtcgttca
     2701 gcctggaccg ggcttcggac caggcgtcgg gccgagacgc cgcactctcg aaactgctgg
     2761 cggaagcctc ggagggggtg cgcactcgcg accacgcccc ggctgcacgc gccctggtcg
     2821 aggcttccca gaaattcgct gaggccaaca accagcgcga ggacgcggtg gccctggaga
     2881 acgtgctggc gaaactgaac aagggccgcg aacagatagc gcaggcaggc aggccgcaga
     2941 cgaagaaggc gcaggtagga ttccggcgca gggggtccgc ggccggtagc aacactccca
     3001 cgcccgacct ggaggacttc caacccacgg ccgatgcgca acagccgggc caggcgggcg
     3061 cgaggggcaa ctcaagcacg agcggcctcg gccccgtggt gggagagagt aacgtggcgt
     3121 cttatggcgc atcgcagtcg tccaaggata cgggcggcca gcctgcaaag acaggcggcg
     3181 aggatggcgg tggcccaccc ggcagcggtc cagggggcgg aagcgatggg caggacagcg
     3241 gcca
//
LOCUS       DASRWZ010000408         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_514361, whole genome shotgun sequence.
ACCESSION   DASRWZ010000408 DASRWZ010000000
VERSION     DASRWZ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514361"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..>2696
                     /locus_tag="VFH60_06335"
     CDS             151..>2696
                     /locus_tag="VFH60_06335"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HET6313434.1"
                     /translation="MSGVAEATSDERGYFSFSLAQLPAGSLSASDSGQPVTLSVREGA
                     YAPWSIREAIFYPGDTLRIYPRLESNRSSPTDLVAYKRQALAASFSFAHSMEAASLDQ
                     PFTDNTTAAPPQAPPATIRVYRTATGVVEVVPFRDYVKHVLPSEWIPTWPSEALKAGA
                     MAVKSYAWYWVSRGGKQVSLGADVKDNVDDQVYDPNVSYASTDAAVDATFNYAMTRNG
                     ALFQAQYCAGSYGPDPSGDCPWPNQYMTQWGSAYYGDAGKPWGWILQFYYRGATITPD
                     PPGGGYDGAPVPTSAPQPQPTRPAQPPVPPAPAPPSTFTVGQGATKPEIFQEAYERNG
                     GAQVLGRPTSAVRWWLPYVTEHNVVAQSFSGPQGDGRLWLVYEVLKSTNDTGHAAYML
                     SGDIAAAYAEHDPPGPEWVGAPTSDTFRGGSELGNMPAQGFRKGLLSGSGQSVQFTPW
                     PETFSAWKAEYFPRHHVSAPQAAPARSLRGWPALVRDVASPNFDWPAGARIPESLGMG
                     AREWSIQFTRDFELVGGSYDLTVAADSGVRLWVDGLLAVDGWHWDGLHTEGYNIDLAT
                     GTHNVRIQYFSLDSAARLEFNMAPRGASPPPAPTVQAPPPEKKTGNANLRTSVRWLGR
                     QSAPNDSWVQPLTLSLSDPASASIVGKYQSTTDRNGVAFFQGLPEGTYNVHVKGPHSL
                     QSARASILLSNNTTAEADMKAQIEGDVDGDNCVTVADFSVVQAMLGANENSSGFNPAA
                     DLNSDGEVTLSDVSLLRSGFDMCGDISADGQFSAMSTNLAPALAPWLNPERLPRTLEM
                     SLQASGTSVKPGEIVELDVIAEAGVQPVDGASFVLRFDPAQLAPVDAAGFP"
BASE COUNT          534 a          893 c          798 g          471 t
ORIGIN      
        1 aggccatctc aagccacgtc cggcgagttg cctgccatgc aggttatttc tgatggaagt
       61 gcggaagcta ataaaaacga aaagccggcc gtcgaaggct gggtaactga taagtcgtcc
      121 aggcaggccg tacagggcgc agtcgtcacc ttgagcggtg tagctgaagc cacctctgat
      181 gagcgtggat acttttcatt ctcactggcg caacttccag caggcagcct ctcggcctcg
      241 gatagcggcc agccggtcac tctatctgta agagaaggcg catacgcccc ctggtcaatc
      301 cgcgaagcca tcttctatcc cggagacacc ctgcgtatat atcccaggct ggaaagcaac
      361 aggtcctcgc ctaccgacct tgtcgcgtat aagcggcagg ccctcgccgc atcgttcagc
      421 ttcgctcaca gcatggaggc tgcgagtctt gaccagcctt tcacggacaa cacaacagca
      481 gccccacctc aagcgccacc tgccaccatc agggtctacc gcaccgctac aggagtggtg
      541 gaggtggtgc ccttccgcga ttacgtgaag cacgtcctcc ccagcgagtg gatacctacg
      601 tggccttctg aggcgctcaa ggcgggcgcg atggccgtca agagttatgc gtggtactgg
      661 gtgagccggg gcgggaaaca agtgagcctg ggcgcggacg tgaaggacaa cgtggacgac
      721 caggtgtacg acccgaacgt cagctacgcc agcaccgacg ccgccgtaga cgccaccttc
      781 aactacgcga tgacccgcaa cggggcgctc ttccaggccc aatattgcgc cggttcttac
      841 ggccccgacc cctccggcga ttgcccctgg cccaaccaat atatgacgca gtggggttcg
      901 gcctattacg gcgacgcggg caagccctgg ggctggatac tccaattcta ttataggggc
      961 gcgaccatca cgcccgatcc tccgggggga gggtatgacg gcgcgcccgt gcctacctcc
     1021 gcgccacagc cgcaacccac gcgccccgca cagcctcccg tgccgcccgc ccctgctcct
     1081 ccatcaacct tcaccgttgg gcagggcgct acgaagcctg agattttcca ggaggcatac
     1141 gagcgcaacg gcggcgcgca ggtgttgggc cgtcccacga gcgcggtcag gtggtggctg
     1201 ccctacgtga ccgagcacaa cgtggtcgca cagtcattca gcggccccca gggagacggc
     1261 aggctctggc tggtatacga ggtactcaag agcacgaacg acaccgggca cgcagcctac
     1321 atgctctccg gtgacatcgc tgccgcatac gccgagcacg atccccccgg cccggaatgg
     1381 gtaggcgcgc ctacttcaga taccttccga ggtggctctg aattagggaa catgccagcg
     1441 cagggcttcc gaaagggcct gttatcaggc agcggtcagt cggtgcagtt cacgccctgg
     1501 ccggagacct tcagcgcgtg gaaagccgaa tacttcccca ggcaccacgt ttccgcaccc
     1561 caggctgccc ccgcccgttc tctccggggc tggcccgcgc tggtacgtga cgttgcctct
     1621 cccaacttcg attggcccgc cggtgctcgt ataccggaaa gcctcggcat gggcgcacga
     1681 gagtggtcca ttcagttcac cagggatttc gagctagtgg gcggttccta cgacctcaca
     1741 gtagcggcag atagcggcgt gcgcctgtgg gttgatgggc tgctggcagt agacggctgg
     1801 cattgggacg gcctccacac agagggctac aatatagacc tggcaactgg cacccacaac
     1861 gtccgcatcc agtacttcag cctggatagc gccgccaggt tggagttcaa tatggctcca
     1921 cgaggcgcat cgccacctcc tgcgcctacc gtgcaagccc cgccccctga gaagaagact
     1981 ggtaacgcca acttgcgtac cagcgtgcgc tggctcggta ggcaatcggc ccctaacgat
     2041 agctgggtgc agcccctcac cctctcctta tcagaccccg ccagcgcctc gattgtcggt
     2101 aagtaccaat caacaactga ccgcaatggc gtggcgttct tccagggcct gcccgaaggc
     2161 acctacaacg tgcatgtgaa gggaccgcac agcctccaat cggcgcgggc ctccatactc
     2221 ttgagcaaca acacgacggc tgaagccgac atgaaagcgc agatcgaggg ggacgttgac
     2281 ggcgacaact gcgtaaccgt cgctgacttc tcggtagtgc aggcgatgtt gggagccaac
     2341 gagaactcgt ccggcttcaa ccctgccgcc gacctgaaca gcgacggcga agtgacgttg
     2401 agcgacgtta gcctgctgcg ctccggcttc gacatgtgcg gcgatatttc ggcggacggc
     2461 cagttcagcg ccatgagcac aaatctcgca cccgcgctgg ccccctggct caaccctgag
     2521 cgtctccccc gcacgctaga gatgtcccta caggcatcgg gcacttccgt gaaaccaggc
     2581 gagatcgtag agctggatgt gattgcagag gcgggcgttc aacccgtaga cggcgcatcg
     2641 ttcgtgctgc gcttcgaccc agcccaactt gcgcccgttg acgccgcagg gttccc
//
LOCUS       DASRWZ010000409         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_308942, whole genome shotgun sequence.
ACCESSION   DASRWZ010000409 DASRWZ010000000
VERSION     DASRWZ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308942"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VFH60_06340"
     CDS             complement(<1..670)
                     /locus_tag="VFH60_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018977451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313435.1"
                     /translation="MLEGQRVVSGTFSSRQAAETAIRELRDLGVPADAISVISREEDQ
                     ADLEVAAGASREVIGDEALAYRASPELPNYEDLPSTVAEQTGTPLDADAVAGSVSMDG
                     AGLARDADLVRRNEADTNADIDIYTDFPDEPGGLNPESPIAAEYEGTVQEPASNRTPA
                     AGTAATGAALGGLGGLLVGLGVLAVPGIGPLLAAGPLAGALGGMLAGGPAGGVIGGLS
                     AIGVP"
     gene            complement(712..1389)
                     /locus_tag="VFH60_06345"
     CDS             complement(712..1389)
                     /locus_tag="VFH60_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313436.1"
                     /translation="MSDNGQDNTGTGPQAGQPGYHTPGQDTTIQSTSEAVPTPDQTSE
                     ERLEDPKPYIEAINATPGEANPALGETVGAQEGSTSTADLLLARESAEERPVRQEGVR
                     PYQDQSSGSRPDEFQDADSGRPNWPDDKQEDPINYMESPRPYMPMGTGATDFYDRSFQ
                     GRVDAEVDRSSTEAEKGESRSGLPHVGLDAQEDKYEQREFERPQPPSDMETFAPGAPN
                     LPPRDDE"
     gene            1830..2699
                     /locus_tag="VFH60_06350"
     CDS             1830..2699
                     /locus_tag="VFH60_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313437.1"
                     /translation="MQYFERAVFELHPENAQPYDVLLSLLGNLYYQTRYPRDSAPQQR
                     ASTDRPLLFPETGKTLGGRFRDYWEANGGLAQNGYPISNEFEQKNTLNNQVYTVQYFE
                     RAIYEFHPENQPPYDVLLRQVGRIEYSNRLPKDCRHLYLGQGAIIAEGKNTVATNQRY
                     KLKSYYLEEIDLPAPLTCDNALVPPEDRLGTISERRTITKYWRLVLTADPSGQPLPVG
                     AMESFMWLDDEVIGAAWSEFWPARGNSRPKALLFYAYVFDWSLFRDGATPWIGYGGRE
                     AALPEPLQLNSVR"
     gene            2774..3802
                     /locus_tag="VFH60_06355"
     CDS             2774..3802
                     /locus_tag="VFH60_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET6313438.1"
                     /translation="MKVIIPAAGYGTRMRPHTHTRPKPLVPVAGRPSLALVLDALAPL
                     KIEEIIIIVGKMGEQMEEYATKHYPQYRFRFVEQKVMRGQADAIALTEPYIEGDVLTL
                     FVDTLFEADLTVVNDLKDADGAIFVSEVEDPSRFGIAVLGEDGHVRKMVEKPAKPESN
                     LALVGLYYFKDSTALFRGIHRLMASGRSLKGEYYLADAVQEMIDSGAKFRTFPVEVWE
                     DTGTWDATLHTNRYFLRKMDPNTEPYMRGSSLVIPPCFIAEDATVENSVVGPFASISE
                     GATVRDSVVKNSIISPDATVSTMNLFGAMIGERATVEGSYSAINVGDDSVLHTVGRGD
                     ATIDETFK"
     gene            3804..>4409
                     /locus_tag="VFH60_06360"
     CDS             3804..>4409
                     /locus_tag="VFH60_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET6313439.1"
                     /translation="MADWLELSVEVDPEAVEPVSELFARLGYNGGVVVEQPLIVPEGE
                     YPDWSALQQPEIDPNRPISVRTYIPDDAEAPEKRREIEQALWYLGRLRQVGTLGVKPC
                     KEEDWANAWKDYYGIQRMGERIVIKPSWLEYSPKVGDVVLDLDPGMAFGTGLHPTTRL
                     CLAALEEYVRPGTPANRLDLGTGSGILAIAAAKLGGPSARVL"
BASE COUNT          808 a         1402 c         1400 g          799 t
ORIGIN      
        1 ccggcacgcc tatggccgag aggccgccga tcacgccacc cgccgggccc ccggccagca
       61 ttccccccag cgctccggct aacggccctg ccgccagcag tggccctatg cccggcaccg
      121 ccagcacgcc caggcccaca agcaggccac cgaggccgcc cagggccgca cccgtggcag
      181 ccgtaccggc ggcgggagtg cgattgctcg ccggttcctg caccgtgccc tcgtattcgg
      241 cggcgatggg cgactcgggg ttgaggccgc ccggctcgtc ggggaagtcg gtgtaaatat
      301 cgatgtcggc gttggtgtcg gcctcgttac ggcgcaccag gtctgcatcc cgcgccaggc
      361 ccgcgccgtc catggagacg gagccggcca ccgcgtccgc gtcgagggga gtgccggtct
      421 gctcggctac cgtactgggc aggtcctcgt agttgggcag ctcgggtgaa gcgcggtagg
      481 ccaacgcctc gtcgccaatc acctcccggc tggcccctgc cgcgacctcc aggtcggcct
      541 ggtcctcctc gcgggagatc accgagatag cgtcggcggg cactcccagg tcgcgcaatt
      601 ctcgtatcgc ggtctcggcg gcctggcgtg agctgaaggt gccgctgacc acgcgctgtc
      661 cctctaacac tccccccgca gaacttatgc ttctgctatt cccgctgctc atcattcgtc
      721 gtccctgggc ggaaggttag gcgcaccggg cgcgaaggtc tccatgtcgg agggcggctg
      781 cgggcgctca aactcgcgct gctcgtactt gtcctcctgg gcgtcgaggc ccacgtgggg
      841 caggccggag cgcgattcgc ccttctcggc ctcggtggac gacctgtcta cctcggcatc
      901 gacgcgtccc tggaagctcc tgtcgtaaaa gtcggtggcc ccggtgccca tcggcatgta
      961 aggccgtggc gactccatgt agttgatagg gtcttcctgc ttgtcgtcgg gccagttggg
     1021 gcggcccgag tcggcgtcct ggaactcgtc ggggcggctg ccgctcgact ggtcctggta
     1081 aggccgcacg ccctcctgcc tcacggggcg ctcctccgcg gattcgcggg cgagcagcag
     1141 gtccgccgtg ctggtgcttc cctcctgcgc gccgaccgtc tccccaagag cggggttagc
     1201 ctcgccggga gtagcgttta tcgcctcgat gtagggcttg gggtcttcca ggcgctcttc
     1261 gctggtctgg tcgggggtag gtaccgcttc gctggtgctc tgtatcgtgg tgtcctggcc
     1321 gggagtgtgg tagcccggct gcccggcctg cgggcctgtc ccggtgttgt cttgtccgtt
     1381 gtcgctcatg tctggtcact cctgtcggcg cggcactggg cggcccgccg aatagccact
     1441 tggtagtcaa gtgattgcac gcgtcatagg tgcaagatat gtgcccttgc agcctccggg
     1501 cctttgggct aaactggggg ttggccccgg cgatattgtg gcgaaagaac gagggcaggc
     1561 acggcatgac tactcacagg tccgttcact tcagatggat cacgctatgc agcctgctgc
     1621 tgagcgcttt aggagtagag cctacaactc gcgcagaggc tatatcccac cccgccgcgc
     1681 aggaatgccg gaccttccaa gagaccggcc agaccgtatg tggcaggttt ctagagtact
     1741 gggacgagta tggaggtctg ccacagcaag gactgccgct ctcaaaggag ctgcgtataa
     1801 agagcgaagt agatggtagg acgtacttgg tgcagtattt cgaacgggcc gtgttcgagc
     1861 tacacccgga gaatgcccag ccctacgatg tactgctaag cctactgggc aacttgtact
     1921 accagactcg ctacccgcga gatagcgcgc cgcagcaacg ggcgagtacc gaccggccgc
     1981 tcctattccc ggaaacgggt aagacccttg gaggacgctt ccgagattac tgggaggcga
     2041 atgggggcct tgctcagaac ggctatccca tctcgaatga gtttgaacag aaaaacacgc
     2101 tcaacaacca ggtgtacacg gttcaatact tcgagcgtgc catctatgag ttccatcctg
     2161 agaatcaacc gccttacgac gtgcttctca ggcaagtggg caggattgaa tacagtaaca
     2221 ggctgcctaa agattgccga catctttatc tggggcaggg agccatcatc gctgagggga
     2281 aaaatacggt agcaaccaat caacgctaca agttgaagag ctactatctt gaggagatag
     2341 acctgccggc accccttacc tgcgacaacg cactcgtacc tcctgaggac agattaggaa
     2401 ctatatctga gaggcgcacg attaccaagt actggaggct ggtcttgacg gcggaccctt
     2461 caggccagcc cctgcctgtc ggtgccatgg agtccttcat gtggctcgat gacgaggtga
     2521 taggagcggc ctggtccgag ttctggcccg ccaggggtaa cagccgacct aaagccctat
     2581 tgttctatgc ctatgttttc gattggtcgt tgtttcgcga cggagctacc ccctggattg
     2641 gctatggtgg ccgggaagct gctttacctg aaccactgca actcaacagc gtccggtagc
     2701 aagggcttac aggcggacct gcgagcaaac tgaggaatag agtaatcatg agacacacca
     2761 ctacaggagc actatgaaag taatcatccc cgcagccggg tacggcaccc gcatgcgacc
     2821 tcacacccac acccgaccca agcccctcgt gccggtcgcc gggcgtccat ccctagcgct
     2881 cgtgctggac gcgctcgcgc cgctcaaaat cgaggagatc atcatcatcg tcggcaagat
     2941 gggcgagcag atggaggagt acgccaccaa gcactacccg cagtacaggt tccgcttcgt
     3001 cgagcagaag gtcatgcgcg ggcaggcgga cgctatcgcc ctcaccgagc cgtacatcga
     3061 gggcgacgtg ctgaccctct tcgtggacac cctgttcgag gccgacctca ccgtggtgaa
     3121 cgacctgaag gacgcggatg gggctatttt cgtgtcggag gtggaagacc cctcgcgctt
     3181 cggcatcgcc gtgctggggg aggacggcca cgtgaggaag atggtcgaga agcccgccaa
     3241 gccggagagc aacctggccc tcgtcggcct ctactacttc aaggactcca cggccctctt
     3301 tcggggcatc cacaggctga tggcttcggg ccgcagcctc aagggcgagt actacctggc
     3361 cgacgcggtg caggagatga tcgattcggg cgcgaagttc cgcaccttcc ccgtcgaggt
     3421 gtgggaagat accggcacct gggacgccac actgcacacc aatcgctact tcctgcgcaa
     3481 gatggacccc aacaccgagc cgtacatgcg cggctcctcc ctcgtcatcc caccctgctt
     3541 catagccgag gacgccacgg tcgagaactc ggtggtaggc cccttcgcct cgatttcgga
     3601 gggggccacc gtgcgcgact cggtggtgaa aaacagcatc atcagcccgg acgccacggt
     3661 ctccaccatg aacctctttg gcgcgatgat aggcgagcgg gctaccgtgg aaggctccta
     3721 cagcgcgatc aacgtgggcg acgactcggt gctgcacacg gtcggccggg gcgacgccac
     3781 gattgacgag acgttcaaat aagatggcag actggcttga gctgagcgta gaggtagacc
     3841 ccgaagcggt agagccggtg agcgagctat tcgcccgtct tgggtacaac ggcggggtgg
     3901 tcgtggagca gccgctaatc gtgccggagg gggagtaccc cgattggtcc gccctgcaac
     3961 agccggagat cgaccccaac cgccctatta gcgtgcgcac ctacataccg gacgacgccg
     4021 aagcccctga gaagcggcgg gagatagagc aggcgctgtg gtacctgggc aggctgcggc
     4081 aggtgggcac gctcggcgtg aagccgtgca aggaggagga ctgggccaac gcctggaagg
     4141 actattacgg catacagcgc atgggcgagc gcatagtgat aaaaccctca tggctcgaat
     4201 atagccctaa agtgggtgac gtggtgctcg acctcgaccc cggtatggcc ttcggcaccg
     4261 gcctccaccc caccacccgc ctgtgcctcg ccgcgctcga ggagtacgtg cggcccggca
     4321 cccccgccaa cagactggac ctgggcaccg gctccggcat actggctatc gcggcggcca
     4381 agctgggcgg cccctcggct cgcgtgctg
//
LOCUS       DASRWZ010000410         1810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_857428, whole genome shotgun sequence.
ACCESSION   DASRWZ010000410 DASRWZ010000000
VERSION     DASRWZ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857428"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1810)
                     /locus_tag="VFH60_06365"
     CDS             complement(<1..>1810)
                     /locus_tag="VFH60_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313440.1"
                     /translation="LTTLYVAVGARGYSTTVQSNLAENGANGMYRATVPASGCPAEWD
                     SISSFGNGWPNGSSSGIPQYQAGGNQLGRIDIAIAPSDEKVLYAVVQAINAPGIRGAP
                     LGVWRTTDGGESWVQRATAAAASPNTDWSGCDDPGTQSWYNQHIEVDPNNPNVVLVDA
                     IDIFKSSNGADTFTNITCGYTQNTTKNVHVDQHALEFVPGSSSKMLAGNDGGVYLSLN
                     MTATTPTNITFTQLNDSLNTIEFYSGDITANFAYSTTPGINAGAQDNGSSVYQWSSNN
                     VGPAMWQLESGGDGMYARIEPMLEQRWYQESQNGVIYRSDTGPHGEYVYSFGAWTTGL
                     AGPIIDRLSFVFPYEMYKYDCPLTGCTHMIAGTHRVWETLTGAVPSESWYPNSPDLTK
                     NTLADRSFINQLSYAVSLSTTAIVGTNDGNVQYGFNLGLGVPNSATWVNVTGNNTALP
                     NRPILDVTTDPINPLIGYAAVGGFDQNTPSTPGHVYQVTCSLNSVTFSWEDKSGNLPN
                     IQVDSIMVTPKFRQQVFAGTDWGFYFTDDIDAASPVWYRFNIGMPNVMIWDMAIDKGF
                     TTLAAFTRGRGAYAWPLPSGPFGDDLPTATPTGTATA"
BASE COUNT          312 a          520 c          601 g          377 t
ORIGIN      
        1 tcgcggttgc tgtaccggtc ggcgtcgcgg tcggcaggtc gtcaccgaac gggccggaag
       61 gcagcggcca ggcgtacgcg ccacgccccc tggtaaaggc cgccaacgtg gtgaagccct
      121 tgtcaattgc catgtcccag atcatgacgt tgggcatgcc gatgttgaag cggtaccaca
      181 cgggggatgc ggcgtctatg tcgtcggtga agtagaatcc ccagtctgtg cccgcgaaca
      241 cctgctggcg gaacttgggg gtgaccatta tggagtccac ctggatgttg ggcaggttgc
      301 cgctcttgtc ctcccacgag aaggtgacag agttcaagga gcaggtgacc tgatagacgt
      361 gtcccggcgt tgagggagta ttctggtcga agccgcccac agccgcatag ccgatgagag
      421 ggtttatagg gtcggtggtc acgtccagga taggacggtt cggcagcgcg gtattgttgc
      481 ccgtcacgtt gacccaggta gccgagttcg gaacgcccag gcccaggttg aaaccgtact
      541 gcacgttgcc gtcgttggtg cccacgatgg ccgtggtaga gagcgatacc gcgtagctaa
      601 gctggttgat gaacgagcgg tcggctagcg tgttcttcgt caggtctgga ctgttggggt
      661 accagctttc agacggaaca gcgccggtca gggtttccca cacccggtga gtgcccgcta
      721 tcatatgcgt gcagccggtg agcgggcagt cgtacttgta catctcgtag gggaatacga
      781 agctaagccg gtcaattatc ggccccgcga ggcccgtggt ccaggctcca aacgagtaga
      841 catattcccc gtgaggcccc gtgtcggagc gataaatcac gccgttctgg ctttcctggt
      901 accagcgctg ctccagcata ggctcgatgc gcgcgtacat gccgtcgccc ccgctctcca
      961 gttgccacat ggcagggccg acgttgttgc tggaccactg gtagaccgag gagccgttgt
     1021 cctgggcccc ggcgttgatg cccggcgtgg tgctatacgc gaagttagcg gtgatgtcgc
     1081 cggagtagaa ctcgatggtg ttcaggctgt cgttcagctg tgtaaaggta atgttggtgg
     1141 gcgtagtggc agtcatgttg agactcagat acacgccacc gtcgttgccc gccagcatct
     1201 tgctcgaaga gccgggcacg aactccagcg cgtgctggtc aacgtgcacg ttcttggtag
     1261 tgttctgcgt gtagccgcag gtgatatttg tgaaggtgtc cgcgccgtta gacgacttga
     1321 aaatgtcgat ggcatcgacc agtaccacgt tgggattgtt cggatcgacc tcgatgtgct
     1381 ggttgtacca gctctgagtg ccggggtcgt cgcagcccga ccagtcggtg ttaggcgagg
     1441 ccgcggcggc agtggctcgc tgcacccagc tctcgccgcc gtcggtcgtg cgccacacgc
     1501 cgaggggcgc gccacggatg cccggcgcgt tgatcgcctg caccaccgcg tacaatacct
     1561 tctcatcgct aggagcgatt gcgatgtcaa tgcggcctag ctggttgccg cccgcctggt
     1621 actgagggat gccgctggaa gagccgttgg gccagccgtt cccgaacgag cttatggagt
     1681 cccattccgc cgggcaaccg ctggccggca cagtggccct gtacatgccg ttggccccgt
     1741 tttccgcgag gttgctctgc accgtggtgc tgtagccacg ggcacccacc gctacgtaca
     1801 gggtggtaag
//
LOCUS       DASRWZ010000411         1847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_857449, whole genome shotgun sequence.
ACCESSION   DASRWZ010000411 DASRWZ010000000
VERSION     DASRWZ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857449"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..736
                     /locus_tag="VFH60_06370"
     CDS             143..736
                     /locus_tag="VFH60_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HET6313441.1"
                     /translation="MPTTPLTHEALIEAFTERARAVAALVSTVRSVEEAAALIASSEV
                     PTVTGRHTATSQALAHYAGLREALQGRGIVLRLAEELDTGVGGQSATAASMAGDVGIV
                     LCRAGVAETGSLLSVEETLAARLLGMLADTVYALLPAPGIVPTLDEMGHTLAQLSREG
                     TRYVSLITGPSRTADIERVLTIGVQGPKMLHIIILNA"
     gene            762..>1847
                     /locus_tag="VFH60_06375"
     CDS             762..>1847
                     /locus_tag="VFH60_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET6313442.1"
                     /translation="MKRIPGTGIIRRVPRGKRSPSNVEESPHLPLPFEERYRRALSDA
                     QLQRNLLNFQRSWRDSRDGAWAGYEENPLTPRFEESEGAPNANPLVHLPDSPGTAEFE
                     ALRDRLAAIKDEVIAHLPEYVDRFQAAAEANGVRVFRAKDADEANRYVLDLCARKGIT
                     HVVKSKTMVSEETNLNAALEEHGIEAVETDLGEWIVQLAHERPSHMVMPAIHKSRQQV
                     GRLFTDTTGYEVSHEDIAEQVGVARRELRRDFLEAGLGINGANALIAESGTVMFIENE
                     GNARLVTSLPRVHVVFAGIEKLVPDYPAAMLQLRLLARSATAQAITSYTSFVSGPPEP
                     GKEMHVVLLDNGRMQMREHPLIKEALRC"
BASE COUNT          342 a          607 c          613 g          285 t
ORIGIN      
        1 aactccacag ggcgtccgtg tagagccaac ccatctcacg agagctacac accagcccgc
       61 cggcccactg gactgggcaa caggcacgct tacgatatag aatgtgtggc aaccgtccac
      121 gcaccccagg aggccctcga ccgtgcccac aactccactg acccacgaag ccctcatcga
      181 agccttcacg gagcgcgccc gcgcggtggc ggccctggtc agcacggtgc ggagcgtgga
      241 ggaggccgcc gcgttgatag cctcctcaga ggtgcctaca gtcacgggcc gccacaccgc
      301 cacgtcccag gcgctcgcgc actatgccgg gctgcgtgag gctctgcaag ggcgtgggat
      361 agtgctcagg ctggcagagg agctggatac cggcgtgggg ggtcagtccg ccaccgcggc
      421 gagtatggcg ggggacgtgg gcatcgtgct atgccgcgcg ggcgtggccg agaccgggtc
      481 gttgctctcc gtcgaggaga ccctggccgc tcgcctgctc ggcatgctgg ccgacaccgt
      541 gtacgccctc ctccccgccc ccggcatagt gcccaccctc gacgagatgg gccacaccct
      601 cgcccagctc agccgcgaag gcacccgtta cgtctccctg ataaccggcc ccagccgcac
      661 cgccgacata gagcgggtgc tcaccatagg cgtgcagggg ccgaagatgc tgcatatcat
      721 aattcttaat gcttaattct aaattggctg aaggggcaat catgaagcgt attccgggga
      781 ccggcattat ccgtagggtg ccgaggggca agcgttcgcc ttccaatgtg gaggagtcgc
      841 cgcacttgcc gctgccattc gaggagaggt ataggcgggc gttgtccgac gctcagttgc
      901 agcgcaatct gcttaacttc cagcggtcgt ggcgcgactc gcgggatggg gcctgggccg
      961 ggtacgaaga gaacccgctt acgcccaggt tcgaggagtc ggagggcgcg cccaacgcta
     1021 acccgctggt gcacctgcct gatagccccg gcacggctga gtttgaggcg ttgcgggaca
     1081 ggctggccgc tatcaaggac gaggtgattg cgcacctacc ggagtacgta gaccgcttcc
     1141 aggcggcagc cgaggctaac ggcgtgcggg tcttccgggc gaaggacgcg gacgaggcca
     1201 accgttacgt gctggacctg tgcgctcgca agggtatcac ccacgtcgtc aagtcgaaga
     1261 cgatggtatc cgaggagacc aacctgaatg cggcgctgga ggagcacggc atagaggcgg
     1321 tggagaccga cctgggcgag tggatagtgc agctcgcgca cgagcgaccc tcgcacatgg
     1381 tcatgcccgc gatacacaag agccgccagc aggtgggccg gcttttcacc gacaccaccg
     1441 gctacgaggt cagccacgaa gacatagcgg agcaggtggg cgtggcgcgg cgggagctac
     1501 ggcgcgactt cctggaagcc gggctgggca tcaacggggc caacgccctc atcgcggaga
     1561 gcggcacggt catgttcatc gaaaacgagg gcaacgcccg cctggtgact tccctgcccc
     1621 gcgtgcacgt cgtgttcgcc ggtatcgaga agctggtgcc cgactacccc gctgccatgc
     1681 tgcaattgcg cctcctcgcc cgctcggcta ccgcgcaggc gatcacctcc tacaccagct
     1741 tcgtgtcagg cccaccggag ccgggcaagg agatgcacgt cgtcctgctc gacaacgggc
     1801 gcatgcagat gcgcgagcac ccgctcatca aggaggccct gcggtgc
//
LOCUS       DASRWZ010000412         6591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_651506, whole genome shotgun sequence.
ACCESSION   DASRWZ010000412 DASRWZ010000000
VERSION     DASRWZ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651506"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1936
                     /locus_tag="VFH60_06380"
     CDS             149..1936
                     /locus_tag="VFH60_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313443.1"
                     /translation="MLFNTQRYHRALRFAAIVTLLMSLALAYQGGSNPVQAQGRVLEI
                     GPIATMAMDDTGSGWAWTEPSGGTRGEPKLLRIAGNTYRAATINSPDAGQLVRNTDVL
                     YDIDLVSQIGEGWAIGAGGGVRIWRLQGGVWRNYPHNLGRDVVLKDLAVSNDGRDGWI
                     TGTRSNLRGVLLRLRDGQWVEQAQTSGPELQYVAISPNGAHAWAVGQVPVPGGGSAIA
                     VYQWLNGAWMIRNTFQGAPVVVAGQIAVNALTVDDNGNGWLLSPTTLHATRDAYITRL
                     TNESLEQVAVDLPLTAAGGGNIISVYLHDAQVNAVGNGWITGAAQVAAQSGRPQTLPV
                     LIRVVGSRFQEISTATLGIGPTLFDPTIVRLSPLAITPDGSHTWLASDLSAEFLSLLE
                     ISEAWVQAAPSVAVTLPGPGFCFQESRYCLRGAFARYWAANGGLRQFGFPITPEVTER
                     LGNRTYTVQYTERARLEYHPENRPPNDVLLGLLGNALADPRLGENPFKPAAPNNSPGF
                     RYFPQTGHNVGPPFLALWESRGGMPVFGLPRSEAFEEKSAIDGKTYLVQYFERNRLEY
                     HPENVGTGYELLLGLLGLEHFQKTYGYKP"
     gene            2024..3070
                     /gene="add"
                     /locus_tag="VFH60_06385"
     CDS             2024..3070
                     /gene="add"
                     /locus_tag="VFH60_06385"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET6313444.1"
                     /translation="MSLQSYLAAVPKAELHVHLEGSISPATLLVLAERNGIVLPSNTV
                     EGLREYFRFRDFDHFIEVYGVISRCLKTEDDFELIAYEFGTEMARQNVRYAEVTFSCA
                     FHHHHGIPDDAYMGGLLRGRDRARNDFGVHINWVFDIIRSVTDPALREKYADYTVSVA
                     IENMSNGVVALGLGGGEVGYPPEMFVSHFERAIKAGLHSDPHAGEVAGPESVWGALRL
                     LGAERIGHGVRSIEDPALVEYLAEHRVPLEVNPTSNVYLGVYPSLQAHPFRRLYEAGV
                     PLTVNSDDPPLFNTTLNDEVALLASAFNFDISTIDDLLLNGIHHSFLPHERKQEMEQA
                     FRREMAELKAAHLV"
     gene            3087..3593
                     /locus_tag="VFH60_06390"
     CDS             3087..3593
                     /locus_tag="VFH60_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6313445.1"
                     /translation="MPSKPRPQVWVPVAMVLLVEPGGRVLLQHRDDDAQYAPGKWALP
                     GGAMEGGETPEEAARREVLEETGLAISGPLALFAYYVAYADSNNVWHPAVSIEAALAA
                     NPSIIREAYIFYAGTDARQEDVLLGEGQAMVFQTPDAALALDLGNTARRVLPQFFASP
                     EYAQLIRK"
     gene            3689..5155
                     /locus_tag="VFH60_06395"
     CDS             3689..5155
                     /locus_tag="VFH60_06395"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HET6313446.1"
                     /translation="MPVQAADAPAVCKEITEAVLAAIWNEQAPLRGPMWDCEGSPVAV
                     VYRGRWTAGSGPDFEGAMLALGESGTLTGGSVEMHLRCGDWWAHGHHADPRYNSVALH
                     VVLWPLGARPVTRADGATVPTLVLADYITMPTAQLLESVTPLIPNLGALSEEPCWQRT
                     QDWPLERLLDRIDEAGDTRLLNKAAQMEAGLSERGPEEVFYRGIMDALGYSANREPMR
                     RLADALPVSQLLTLPLSHDEGERAALLEAVFLGAAGFLPSQSLPAGPLDWLSSQYAEE
                     AERLWSTYAPLLDVPLDRPLTGGWITNRVRPANSPLRRLAAAARILARLLWAQGGMLG
                     PFIEAATTALNPAALSKHWTDLLAVPAEGYWAFHDGFGHALPAHNAKEGVALVGSSRA
                     ADTVVNVAMPLLLAVADRESNDVLREKVLSAYAAFPRLSENEVTRAMSEEALGPRAGK
                     AISGARRQQGLIHLYRLYCQARRCYECPVSGLRPARQS"
     gene            5347..6264
                     /locus_tag="VFH60_06400"
     CDS             5347..6264
                     /locus_tag="VFH60_06400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6313447.1"
                     /translation="MAGTYSEGSHSAVVLVVDPDPINQRAFRDRLVRLGYTARSADDA
                     DSAQAIVESTPPDVVIATHSSTQANDWPALQINLGNWGIPYVELAEADMASEAASGLD
                     DADLRERIREALRSRKLQDALVAENTRLAAERLYDPITGLFNRRYVMIRVEEEVKRSA
                     RHAYPLSCLLLDIDKLDDVNERFGNHVGDAILRDMAHIITRTLRATDIVARYRDDEFI
                     SLLTDTDSTGAQVAANRLRDAVAAYKYTGTTDGEQVSLTASVGVAYWEPVNRPGEGTW
                     EPQLIALAERALRAAKMSGPNRLVMLQAA"
BASE COUNT         1240 a         2142 c         2063 g         1146 t
ORIGIN      
        1 acccattcct gggagaaaac gttaaagata cggcgtacgt taatactaga tgtatattga
       61 catttaggcg tattctgtgc ttctaaacta gtatctttaa taccagaagg attgaaggtg
      121 cttccgttta tctagaggag tacgtctgat gctatttaac actcagagat atcaccgcgc
      181 gctaagattc gctgccatag tcaccctgct tatgagcctg gcgcttgcct accagggggg
      241 cagcaacccc gtgcaggccc aggggcgggt gttggagata gggccgattg ccactatggc
      301 catggacgac accggctccg gctgggcgtg gacggaaccc agtggtggga cccgggggga
      361 gcctaagctt ttacgcatag ccgggaacac gtatagggca gccaccatca acagcccgga
      421 cgcggggcaa cttgtgcgaa acaccgacgt gctctacgat atagacctcg tctcgcagat
      481 aggcgaaggc tgggcaatcg gtgccggggg cggggtgcgc atctggcgtc tgcaaggcgg
      541 cgtgtggagg aactacccgc ataacctcgg ccgggacgtg gtcctgaaag acctcgccgt
      601 gagcaatgac ggcagggatg gctggatcac gggcaccagg agcaatctca ggggcgtact
      661 gttgcggctg cgcgacgggc agtgggtgga gcaggcgcag acctccggtc cggaattgca
      721 gtacgtggcg atcagcccca acggagcgca cgcctgggcc gtcggccagg tcccggtgcc
      781 cggaggaggc tccgcaatcg ccgtctacca gtggctgaac ggcgcgtgga tgatacgcaa
      841 cacctttcag ggtgcgcccg ttgtcgtagc ggggcagata gcggtcaacg ccctcaccgt
      901 ggacgacaac ggcaacggct ggctcctcag ccctacgact ctccatgcca cccgcgacgc
      961 ctacatcacc cgtctgacga acgagagcct ggagcaggtg gccgtggact tgccgttgac
     1021 agcagcggga ggcggtaaca tcatttcggt gtacctgcac gacgcgcaag tgaacgcggt
     1081 gggtaatggc tggatcaccg gggcggcaca ggtggcggcg cagagcggcc gcccccagac
     1141 ccttcccgtg ctcatccggg tggtggggtc ccggttccag gaaatatcta ccgccacgct
     1201 gggcatcggg ccgaccctct tcgacccgac aatcgtacgg cttagccccc tggcgataac
     1261 gcccgacggc agccatacgt ggttagcgtc ggacctgagc gccgagttcc tcagcctgct
     1321 tgagataagc gaagcctggg tccaggcggc cccgtctgtc gccgtcacgc tgcccggccc
     1381 cggcttctgc ttccaggagt cgcgctactg cctccgcggg gccttcgccc gctactgggc
     1441 ggccaacggt ggcctgaggc agttcggctt tcccattacg cccgaagtta cggagcggct
     1501 gggcaacagg acctacacgg tgcagtatac cgagcgcgcc cgcttggagt accaccccga
     1561 gaacaggccg cccaacgacg tgctcctcgg cctgctgggc aatgcgctgg ccgacccgcg
     1621 cctgggagag aaccccttca agcctgccgc acccaacaac tcgcccggct tccgctactt
     1681 cccgcagacc gggcacaacg tcggaccgcc cttcctggcg ctgtgggaga gccggggcgg
     1741 catgcccgta ttcggcctgc cccgctccga ggccttcgag gaaaagagcg ccatagacgg
     1801 caagacctac ctggtgcagt acttcgagcg taaccgcctc gagtaccacc ctgaaaacgt
     1861 aggcaccggg tatgagctac tactcggcct cctcggcctg gaacatttcc agaaaaccta
     1921 cgggtataag ccttagctaa cgcgtaacac gtgacgcgta acacgtaatg gagtactaac
     1981 tcattacgtg ttacgcgtta cgtgtttctc cttcggagaa accatgtcgc tgcaaagcta
     2041 ccttgccgcc gtgcccaagg cggagttgca cgtgcatctt gaggggtcta tctcgcccgc
     2101 cacgctgttg gtgctggcgg agcgcaacgg catcgtgctg ccctccaaca cggtggaggg
     2161 gctgcgggag tacttcaggt tccgcgactt cgaccacttc atcgaggtgt acggggtaat
     2221 ctcgcgctgc ctcaagaccg aggacgactt cgagcttatc gcctacgaat tcggcaccga
     2281 aatggcccgc cagaacgtgc gctacgcgga ggtaactttc agttgcgcct tccaccacca
     2341 ccacggcatc cccgatgacg cctatatggg cggcctgctg cgggggcgag accgcgcgcg
     2401 taacgacttc ggggtgcaca tcaactgggt gttcgacatc atccgcagcg tgaccgaccc
     2461 ggcgttgcgg gaaaagtacg ccgactacac ggtgagtgtg gctattgaga acatgagcaa
     2521 cggcgtggtg gcgttgggcc tgggcggcgg ggaggtaggc tacccgcccg agatgttcgt
     2581 gagccacttc gagcgggcta tcaaggcggg gctgcacagc gacccccacg cgggcgaggt
     2641 ggcagggccg gagagcgtat ggggcgcgtt gcggctgctg ggtgccgagc ggatagggca
     2701 tggcgtgcgc tccatagaag accccgcgct ggtggagtac ctggccgagc accgggtgcc
     2761 gctggaggta aaccccacca gcaacgtcta cctgggcgtc taccccagcc tccaggccca
     2821 ccccttccgc cgcctgtacg aggccggggt gccgctcacc gtcaactcgg acgacccgcc
     2881 cctcttcaac accaccctca acgacgaggt agccctcctc gcgagcgcct tcaacttcga
     2941 catctccacg atagacgacc tgctgctcaa cggcatccat cacagcttcc tcccccacga
     3001 gcgcaagcag gagatggagc aggcgttcag gcgcgagatg gccgagctga aggcagcaca
     3061 cctcgtatag gagatactgc cgactgatgc cttctaaacc ccgaccgcag gtatgggtgc
     3121 cggtggctat ggtgctgctg gtagagcctg gcgggagggt actgctgcaa caccgggacg
     3181 atgacgccca atatgcccct ggcaagtggg cgctgccggg cggtgctatg gagggcggcg
     3241 agacgccgga ggaggccgca cggcgggagg tgctggagga aacgggcctg gcgataagtg
     3301 gccccctggc gttattcgct tactatgtag cttatgctga cagcaacaac gtgtggcacc
     3361 cagccgtttc gattgaggcc gccctggccg ctaacccctc gattatccgc gaagcatata
     3421 tcttctatgc aggcacggat gcccggcaag aagacgtcct gctcggtgaa gggcaagcga
     3481 tggtgttcca aacacccgat gcggcattgg ccctcgattt gggcaatacc gccaggcgcg
     3541 tgctgcctca gttcttcgcg tcccctgaat acgcccaact aatccgcaag taatttgtcg
     3601 aacaacgccc tcccagccgg aactacctat gtagcccctg ctcccgccgc gagcaaatac
     3661 ggctacaggg gaggcccggc ccatgtgcat gcccgtacaa gctgctgacg cgcccgcagt
     3721 ctgcaaggag ataaccgagg cggtgctggc cgccatctgg aacgagcagg cacccttgcg
     3781 aggccctatg tgggactgcg agggcagccc ggtggcggtg gtgtatcgcg gacggtggac
     3841 ggcggggagc ggacccgact tcgagggcgc gatgctggcc ctgggcgaga gcggcaccct
     3901 caccggaggc tccgtggaga tgcacctgcg ctgcggcgac tggtgggcgc acgggcacca
     3961 cgccgacccc aggtacaact cggtggccct gcacgtggtc ctctggcccc tcggggcgcg
     4021 ccccgtcacc agggccgacg gcgcgacggt gcccaccctg gtgctcgccg actacatcac
     4081 catgcccacc gcgcaactgc tcgagagcgt cacgcccctc atccccaacc tgggcgcgct
     4141 ctccgaggaa ccatgctggc agcgcacgca ggactggccc ctggagaggc tgctggaccg
     4201 catagacgag gcaggcgaca cccgcttgct gaacaaagcc gctcagatgg aggcggggct
     4261 gagcgaacgc ggcccggagg aggtcttcta ccggggcatc atggacgcgc tcggctactc
     4321 ggctaaccgc gagccgatgc gccggcttgc cgacgccctg ccggtgagcc agctactcac
     4381 cctgccgctc agccacgacg aaggggagcg tgctgcccta ttagaagcgg tcttcctggg
     4441 cgctgccggg ttcctgccct cgcagagcct acccgcaggg ccactcgact ggctttcctc
     4501 gcagtacgcg gaggaagcgg agcggctttg gtccacctac gctcctctcc tcgatgtgcc
     4561 gctcgaccgc cccctcacag gcggctggat aaccaaccgg gtgcgtcccg ccaactctcc
     4621 actgcgcagg ctggcagcgg cagcccgcat cctggcccgg ctactgtggg cgcaaggcgg
     4681 catgctcggc cccttcatcg aggcggccac tactgccctc aaccctgccg cgctttcgaa
     4741 acactggacg gacctgctcg ccgtaccggc agaggggtac tgggcgtttc acgacggctt
     4801 cgggcacgcc ctgcctgctc ataacgctaa agagggtgtc gcactggtgg gaagctcgcg
     4861 ggcggcggac accgtagtaa acgtcgccat gccgctcctg ctggcggtgg ccgaccgcga
     4921 aagcaatgac gtattaaggg agaaggtgct ctccgcgtac gcagccttcc ccaggctgtc
     4981 cgagaacgag gtcacccgtg ccatgtcgga ggaagccctc ggcccgcgag caggtaaggc
     5041 catcagtggc gcgcgccgcc agcaggggct tattcacctc taccgcctct actgccaggc
     5101 ccgccgctgc tacgaatgcc cggtctccgg cctgcgaccg gcccggcagt catagggtga
     5161 tgacctagaa ttagtactaa aggcggtgaa gcattaggaa ttgcaccata ttggcttttg
     5221 ctggctgttt agtctacaat agcggggagg gtggcgcgta ccacccggtg ccggcggtgc
     5281 cacctcctat tgagagcacc ggcaccgtcg aaaccgggtc tcacatctcg tatcctaagg
     5341 ggccatatgg cgggaaccta ctctgaaggc tcacattccg cggtcgtgct ggtcgtggac
     5401 cccgatccca tcaaccagcg ggctttccgc gacaggctgg tgcgcctggg ctacaccgcg
     5461 cgctctgccg atgacgccga ttccgcgcag gccatcgtag agtctacccc gcccgacgtg
     5521 gtgatcgcca cccacagctc gacccaggcc aacgactggc ccgccctgca aatcaacctc
     5581 ggcaactggg gtattcctta cgtcgagctt gcagaggctg atatggcctc cgaggcggcc
     5641 tccggcctgg acgatgccga cctgcgcgag cgcattcgag aggccctccg ctccaggaaa
     5701 ttgcaggacg ctctggtagc cgagaatacc cgcctggccg ccgagaggct gtacgacccg
     5761 atcaccggcc tcttcaaccg ccgctacgtc atgatccgcg tggaggaaga ggtcaaacgc
     5821 tccgcccgcc acgcttaccc gctctcctgc ctcctgctcg atatagacaa gctggacgac
     5881 gtgaacgagc gcttcggcaa ccacgtgggc gacgccatcc tgcgcgacat ggctcatatc
     5941 atcacccgca ccctgcgcgc caccgatatc gtggcccgct accgcgacga cgagttcatt
     6001 tccctcctca ccgacaccga ctcgacgggc gcacaggtag ccgccaaccg cctgcgcgac
     6061 gcggtagccg cctacaagta caccggcacc accgacggcg agcaggtaag cctcaccgcc
     6121 tccgttggcg tcgcatactg ggagcctgtg aaccgccctg gcgaaggcac ctgggagccg
     6181 cagctaatcg ccctggccga gcgcgccctg agagccgcca agatgtccgg ccctaaccgg
     6241 ctggtgatgt tgcaggcggc gtgagtaatt actcgtaacg cgtaacccgt aacgcgtaac
     6301 acgtaaccag tatttcgtaa ttatatttcg gcaggctgtt ggtgcggcct gcttttttgt
     6361 ggcctcaagc ttgcactaga tgtcgtgtca gagcaatttt atcgaatgaa agcacccaag
     6421 ccccgccctt gtcatttcga gcgtagcgag aaatctcatc ctacaccggg gtgtggctat
     6481 tttctcaggg gattctatgg tctaaggaag gtggtgcttg ggtgctgagg gatgagattt
     6541 cttgctacgc tcgaaatgac ataaaagggg ccaggtgcgc ttattccagg a
//
LOCUS       DASRWZ010000413         1718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_788686, whole genome shotgun sequence.
ACCESSION   DASRWZ010000413 DASRWZ010000000
VERSION     DASRWZ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788686"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFH60_06405"
     CDS             complement(<1..527)
                     /locus_tag="VFH60_06405"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET6313448.1"
                     /translation="MPPPLAIFLSPHFDDIALSCGGTAARLSRLGARCVGLVVCAAPE
                     PEGAGLSDYAGWQHERWQSASGGGGLSINEVRREEERAAMRLLGLQPVWLDVPDAPYR
                     RNSAGESLYNSDHDLFGRVKGEERRTLVPRIAEEIRRVAREHGGERGRVRVFAPLGVG
                     HHVDHQLAFKAARRLG"
     gene            996..>1718
                     /locus_tag="VFH60_06410"
     CDS             996..>1718
                     /locus_tag="VFH60_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HET6313449.1"
                     /translation="MNGNLREQLRGLVVFAPEVGEALRQGRAVVALESTVIAHGLPHP
                     ANLEVAAGMERIVREGRAVPATIAILDGRVHYGLSGEELERVGTAGDIHKASLRDLPL
                     LLASKASGATTVSATAHLAALAGIKVFATGGIGGVHRNWETTLDISADLPALASTPVV
                     TVCAGAKSVLDLPATLEWLETHGVPVLGYRTGTFPAFYTRTTNPPLRVDLSVETAEEV
                     AAIFNMRQRLNLAGGVLVCQPLP"
BASE COUNT          298 a          538 c          562 g          320 t
ORIGIN      
        1 ccgagccgtc gtgccgcctt gaacgcgagc tggtggtcta cgtggtggcc cacacccagg
       61 ggggcgaaca ccctgacgcg cccccgctcg ccgccatgct cccttgccac ccgccttatc
      121 tcttcagcga tgcgcgggac gagggtgcgg cgctcctcac cctttacgcg gccgaatagg
      181 tcgtggtcgg agttgtagag cgactctccg gcggagttgc ggcggtatgg ggcgtcgggc
      241 acgtccagcc acacgggttg gaggccgagc aggcgcatag cggcgcgttc ttcctcgcgg
      301 cgcacttcgt tgatgctcag acctcccccg ccgctcgcgc tctgccatcg ttcgtgctgc
      361 cagccggcgt agtcggagag gccggcaccc tccggctccg gcgcggcgca caccaccagc
      421 cctacgcacc gcgcccccag cctcgacagg cgcgcggccg ttcctccgca cgacagcgcg
      481 atatcgtcga agtgtggcga gaggaagatg gcgaggggtg ggggcataag gcgagttatg
      541 agttatgagt gctgagtttt gacttgggtt gggtttagag ggttgcgctg gggcgtagat
      601 gtggctgcgg tcctttatac caccacagcg ctgtttgctc caagaaaagg cttgttcttc
      661 ggcatacggt gccggtaatg ctagaaccct gaccattagc tgctatacgc taactcaatg
      721 agagaacgtg gaatacaggc gggtaagccc cttctatgtc atttcgagcg tagcgagaaa
      781 tctcgtcctt cagcacccaa atagcacttt tcctgccgta cagagtccca ggagaaaaga
      841 gccacatccc ggtgtagaac gagatttctc acttcgttcg aaatgacaaa ggaggggctt
      901 gattccggat gtccgacaat ttcgtttttc acatagcttc tggccactga ccactggcca
      961 ctaaccactg accacccgcg tgttatacta ctcgcatgaa cggtaaccta cgggaacagc
     1021 tacgggggtt ggtggtattc gcgcctgagg tgggcgaggc gttgcggcag gggcgggcgg
     1081 tggtggcgct ggaatctacg gtgatagctc acggattgcc gcacccggcg aacctggaag
     1141 tggcggccgg tatggagcgt atcgtgcggg agggtagagc tgtgcccgcc actatcgcca
     1201 tcctcgacgg gagggtgcac tacgggctga gtggcgaaga gttggagcgg gtgggcacgg
     1261 cgggcgatat ccataaggcc agcctgcgcg acctgcccct gttgctcgca agtaaggcca
     1321 gcggggccac caccgtttcg gccacggccc acctggcggc cctcgcgggc atcaaggtgt
     1381 tcgccacggg aggcataggc ggggtgcacc gcaactggga aaccacgctg gacatctccg
     1441 ccgacctgcc cgcgctcgct tcgacgccgg tcgtcacggt ctgcgcgggg gccaagtctg
     1501 tgctcgacct gcccgccacg ctggaatggc tggagaccca cggcgtgccc gtgctcggtt
     1561 accgaaccgg caccttcccc gccttctata cccgcacgac caacccgccc ctgcgcgtgg
     1621 acctctcggt ggagacggcg gaggaagtgg ccgccatatt caacatgcgg cagcgtctca
     1681 acctggcggg gggcgtgctc gtatgccagc ccctgccg
//
LOCUS       DASRWZ010000414         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_34363,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000414 DASRWZ010000000
VERSION     DASRWZ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34363"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VFH60_06415"
     CDS             complement(<1..1423)
                     /locus_tag="VFH60_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET6313450.1"
                     /translation="MLQWVHAALLGGAMSDGQGYAQWLRDGTLALPFVLAVVLYAGRR
                     MLAGTESGGATLAVLYAAVMGAGFLLGQSFPTGGHAQHEADLSTFSILQHALVIMVAA
                     GAYAMFVEGLLKGLGGNLDAALSRLRVPRKLAPVVWPLAALLVAMPALLFTGGGRAAA
                     QTTFPDQVIHPKGTLLLVARAMPDGSFAYVAPDFGANGVRPTIEMTEGETLDITLKNE
                     LSSDVSLHVHGVHYKPESDGTRHSNSIVKPGQQRVYQWKAGAGTSGYWHYHDHVMGDD
                     EGSTGILGGLYGGLVVRRAGDPKPSKTFVIVGHDKTLNGRIYPDTPTYTAREGELVEW
                     LVISHGNRVHTFHLHAHRWITPNRPTDSSPSNAANASSGREDNHILAPGDSFGFMVIA
                     GEGVGPGMWMYHCHFQDHASAMKGFFKVLPAGGAAQMETSRTFPETGKTVSGRFLEYW
                     TQNGGLAQQGFPLSDEFQEKNDLD"
     gene            complement(1693..2952)
                     /locus_tag="VFH60_06420"
     CDS             complement(1693..2952)
                     /locus_tag="VFH60_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HET6313451.1"
                     /translation="MFKRLKLVGAMFVLPWLLTYAVGARYVAAAPQAQAGPQTWTVLV
                     GAEIFTEPGEKSSWHAMRFYPETITINAGDTILFKQNSGVEPHTVTFLGPDKQRPMDL
                     IPEGPGGPDAPPRLVVNPLVAFPSEGNTYDGSKYANSGIMAQGLPTPPQYSLTFTTAG
                     TFDYICVLHSVQLPDGSIMGMKAKVVVQDAGKALPMTPAQVAAETEAQMQADERKALA
                     EEPAAKQAATRPGPNGTTIHHINSGYASEDGLLHYMRFGPSEVTINQGDTIEWSTLGM
                     HNGFHTVTFGGEPEFLLFEPQPAGPPKIVLNPEFFPAGGSTYTGNGYYNSGPIVGPHD
                     PPEAGAKSYSLTFSQPGRYEYICITHYLNGMDGTVTVNAATGAQPGMPRTGSGIELPL
                     LVGAALAGVALTSLGIAIRRRKSGEMI"
BASE COUNT          586 a          970 c         1061 g          640 t
ORIGIN      
        1 cgtcgaggtc gttcttctcc tggaactcat cggagagggg gaagccctgc tgcgccaggc
       61 cgccgttctg ggtccagtat tcgaggaagc ggcctgatac cgtcttgccc gtctcaggga
      121 aggtgcggga agtctccatc tgcgccgcgc cgcccgcggg cagcaccttg aagaagccct
      181 tcatggccga ggcatggtcc tggaagtggc aatggtacat ccacatgccc ggccctacgc
      241 cctcacccgc gataaccatg aagccgaagc tgtcgccggg tgcaaggatg tggttgtcct
      301 cgcggccact gcttgcgttg gcggcgtttg acgggctgga gtcggttggg cggttggggg
      361 taatccagcg gtgcgcatgc aggtggaagg tatgcacgcg gttgccgtgc gagatgacca
      421 accattccac tagctcgcct tcgcgggcgg tgtacgtcgg agtgtcgggg tagatgcgcc
      481 cgttgagggt cttgtcgtgc cctactatca cgaaagtctt gctcggtttg gggtcgcccg
      541 cgcggcgcac taccagcccg ccgtacaggc cacccaggat accggtgcta ccctcgtcgt
      601 cgcccatgac gtgatcgtgg tagtgccagt agcccgacgt gcccgcgccc gccttccact
      661 ggtagacgcg ctgctggccc ggcttcacga tagagttgct gtgccgcgtg ccgtcgctct
      721 ccggcttgta atgcacgccg tgtacgtgca ggctcacatc gctggacaac tcattcttga
      781 gagtgatgtc aagcgtttcg ccctccgtca tctcgatggt ggggcggacg ccgttcgcgc
      841 caaagtcggg cgctacgtag gcgaacgagc cgtcaggcat ggcgcgggcg accaggagca
      901 gagtgccttt agggtgtatc acctggtcgg ggaaagtggt ctgcgcggcg gcgcgtcctc
      961 ctcccgtgaa gaggagcgcg ggcatcgcca cgagcaacgc cgccaggggc cacactacgg
     1021 gcgcgagctt gcgcggcacg cggaggcgcg acagggcagc gtccaggttg ccgcccaacc
     1081 ccttcaacaa gccttctaca aacatggcgt aggcaccggc ggcaaccatt atcaccagcg
     1141 cgtgctgtag gatcgagaat gttgataggt ctgcctcatg ttgggcgtgc ccgccggtgg
     1201 ggaatgactg gcccaggaga aagcctgccc ccatcaccgc cgcgtatagc acggccaagg
     1261 tagcgccgcc actttcggtc cccgcgagca tgcgcctgcc cgcatagagt acgacggcca
     1321 gcacgaacgg caaggctagc gtgccgtccc gcagccattg agcataccct tgcccgtcgc
     1381 tcatcgcgcc gccgagaagc gcggcatgca cccattgtag caaggcagcg ccgccgtatg
     1441 agagtaccag ggcgtgcaga aggggattga tacttataga agtagcccga acgttcaaag
     1501 tagacacctc tctttgttag tttggtatgc ccaggacggt acaatgttgg accgccggtg
     1561 ccctctaatg atagatgaaa gcgaacgcgc ctggggaata caaaaagacg tacaagtagg
     1621 agtacagagc agccgcagca aggctacacc cgctatagtc cgccgggtat agccttgccg
     1681 tagatttgcc gattatatca tctcgccgga cttgcggcgg cgtatcgcta tgcccagcga
     1741 cgtgagcgcc actcccgcca gggcagcgcc taccagcagg ggaagttcga tcccgcttcc
     1801 cgtgcggggc atgccaggct gtgcgccggt cgccgcattc acagtgaccg tgccgtccat
     1861 gccgttgagg tagtgcgtga tgcagatgta ttcgtagcgg cccggctggc tgaaggtgag
     1921 cgagtaggac ttcgcgcctg cttccggcgg gtcgtgcggc cctactatag gcccggagtt
     1981 gtaatagccg ttgccggtat acgtggaacc gcctgccggg aagaactcag ggttgagcac
     2041 gatcttgggt ggccctgcgg gctgcggctc aaacaggagg aactcaggct cgccaccgaa
     2101 cgttaccgtg tggaagccgt tgtgcatgcc cagcgtggac cactcgatgg tgtcgccctg
     2161 gttgatggta acctctgagg ggccgaagcg catgtagtgg agcaggccgt cctcggaagc
     2221 gtagcctgaa ttgatgtgat gaatggtggt gccattgggg ccggggcggg tggccgcctg
     2281 cttcgcggca ggctcctccg cgagcgcttt ccgctcgtct gcctgcatct gcgcttcggt
     2341 ctcggcggct acctgggccg gggtcatcgg caatgctttg cccgcgtcct gcactaccac
     2401 cttagccttc atgcccatga tgctgccgtc gggtaactgc acggaatgga gtacgcagat
     2461 gtagtcaaac gtgcccgccg ttgtgaaagt gagagaatat tgcgggggtg tgggcaaacc
     2521 ctgggccatg atgcccgaat tggcatactt cgagccgtcg taggtgttgc cctccgaagg
     2581 gaaagcaacc agggggttga ctacgaggcg cgggggagcg tcggggccgc cggggccttc
     2641 cggtatcagg tccattggac gctgcttgtc cgggccgagg aacgttaccg tatgaggctc
     2701 cacgccactg ttctgcttga agagtatggt atcgcccgcg ttgatggtaa tggtttcggg
     2761 gtagaagcgc atggcgtgcc aggacgactt ctcgccaggc tccgtaaata tctccgcgcc
     2821 caccagcacg gtccaggtct gcggccccgc ctgcgcctgg ggagcagccg ccacgtagcg
     2881 cgcacccacc gcgtatgtca gcaaccaggg tagaacgaac attgcgccaa ccagtttgag
     2941 tctcttgaac atttagccct ccaattcctc tgtattgtgt gatttggttg tatatgcctg
     3001 cgctcgcgcc cgcttcgcag acgtatgccg ggtgccggaa ggcccacagg ggaggtctga
     3061 gggcaggcgc gctaccggca gctatatagt gctacaggaa aatgctcatt caccagacca
     3121 ataattgctc atttggtgct cattttggcg tgaggggtga gggattagag cctaattatt
     3181 ctcctcggca ccgcccggcg agagttcgct acgctcatat tcgcgggcta cgcaaacgaa
     3241 gtccgcctgc gcggact
//
LOCUS       DASRWZ010000415         4625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1336658, whole genome shotgun sequence.
ACCESSION   DASRWZ010000415 DASRWZ010000000
VERSION     DASRWZ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336658"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFH60_06425"
     CDS             <1..467
                     /locus_tag="VFH60_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313452.1"
                     /translation="LSSMLRYLPLALAAVWLVAQVWGLALFHRYPPHGADGLRELAAT
                     LNTATRPDDLVLVTPPALQAGLTQYYHDDTYGIPANFDLFNLYPPYDPAKWEADSLVA
                     FERHVGKHNRFWLVYRPELDEGGKFLAEVRRRYAATEQRRYVFATLYLFEGR"
     gene            467..2293
                     /locus_tag="VFH60_06430"
     CDS             467..2293
                     /locus_tag="VFH60_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET6313453.1"
                     /translation="MSQRDSRLAAQLAPPALLVGLSAALFAYLLARFPYNGLYGQDAY
                     AYYYQAEALWHELTGQPQLPHAQFTASGLFWPVGYHLHLFPSFLLVWPEAGRVLTLAL
                     AALTPAVVYLLAGAVWHTGGAVPRTVAGLVAWAVLLLTGTYVRTGLSLMSDVPAVFWG
                     TLGVYFSLRAWPPGERPRARRSEWRWGLAAGAALGVAVLVRYSSALLLPALVAYLAAR
                     GYMQRRESRTPREESPPALWRSSFAWAAVGLAVALLPQVAYLLAHEPGPALRGWSPGN
                     IFSNTVTGSDGTTSFSTPMIGFYLLSVLGNVGGGFLSPFCLPALLAGVWILVRQANIS
                     ATILLFIRWLIGILAYSGTMYQTHRFTLSKMPALAVLIGIGAGAAFTQLQIGAQDRRE
                     RAGNGQAVRGLMSALLLVGLGVGLWQGERSAERWVATHAEFNAQEQRVVALAREAAAA
                     SEQAGTPRVVCFGFSAPLYHYTHWPILDFFAHDEADIEGFLSAPGPHLLVLPEESMST
                     QWAGTPPGARWEWIKSTYRLTKQGQAGVFTVYAIGGRQADQGVPSSSPSSSDASGVPT
                     TPANCIAWRSSFDSMKPRACSTVANSSITSRSLRSPATWKRR"
     gene            2789..3589
                     /locus_tag="VFH60_06435"
     CDS             2789..3589
                     /locus_tag="VFH60_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HET6313454.1"
                     /translation="MSKLLRFSTFLAPSIRPVYEAVAGYVGRELGCDTALVEGRSFGE
                     FAEGACDIGFICGLPYVQLAAQDPRPVELLAAPVLQGERYSGRPIYFSDVIVRSDAPF
                     HSFADLRGRSWSYNDPDSHSGYGVTRYHLVRLGETRGYFGSVVAAGFHQESIRMVREG
                     KIDASAIDSQVLAVAMRDDAELARQVRVIGRLGPSSIQPVVAARTLPDSLKADVQAAI
                     LRMHTDAEAVRGLSHGLVERFAPVADADYDDIREMLAATEAVGFGVLR"
     gene            3620..4618
                     /locus_tag="VFH60_06440"
     CDS             3620..4618
                     /locus_tag="VFH60_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018790460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET6313455.1"
                     /translation="MRPKVTVISLGGTIAMTTTDDGGVTPTLDGAALVSAVPGLGEVA
                     ELTAVSFRQLPSAHLGLEDVLALASEVARHIDAGAGGVVITQGTDTLEEVAFALDLLL
                     DREAPVVVTGAMRNPTLASADGPANLLAAVRVAASSQARGLGVLVVLNDEVHAARYVQ
                     KTHTTGTAAFRSPLTGPLGWVVEGRVRVNNRPVARPCVEWEPGGDVAPVVPVALLTVS
                     LGDDGRLVDLVRQAGYGGLVIEAFGAGHVHVPVAYRLKELAGVMPVVLASRTRSGDML
                     RHTYAFRGSETDLLSSGLISAGPLDGPKARVLLSLLLTAGANREQVAGTFAAWAGD"
BASE COUNT          758 a         1459 c         1551 g          857 t
ORIGIN      
        1 gcctctcctc catgctccgg tatctgcctc tggcgctggc tgccgtgtgg ctggtggcac
       61 aggtttgggg cctggcgctc tttcatcgct acccgcctca cggtgccgac ggcctgcgcg
      121 agcttgccgc cacgctcaac acggccacgc ggcccgacga cctggtgctg gtcacgcctc
      181 ccgcgctgca agccggcctt acgcagtatt accacgacga cacttacggg atacccgcga
      241 acttcgacct tttcaacttg tacccgccct acgatcccgc gaagtgggag gcagactcgc
      301 tggtggcatt cgagagacac gtggggaagc acaaccgctt ctggttggtc tacaggccag
      361 agttggacga gggcggtaag ttcctggccg aggtgcggag acggtatgcg gcgaccgagc
      421 agcgccgcta cgtcttcgct accctgtacc tcttcgaggg ccggtgatgt cgcagcggga
      481 tagccggttg gccgcacaat tggcccctcc cgcgctcctg gtggggctgt ccgccgccct
      541 gttcgcctac ttactcgctc gcttccccta caacggcctt tacgggcagg acgcctacgc
      601 ctactactac caggccgagg ccctctggca cgagctgacg gggcagcccc aactccccca
      661 cgcgcaattc accgcaagcg gcctcttctg gcccgtcggc taccacctgc acctgttccc
      721 ctccttcctg ttggtgtggc ccgaggcggg gagggtgctg accctcgcgc tcgcggccct
      781 caccccggcg gtcgtatacc tgctggcggg cgcggtatgg cacaccgggg gggccgtgcc
      841 gcgcacggtc gcggggctgg tggcatgggc cgtgctgctg ctgaccggca cctacgtccg
      901 cacggggctg tcgctgatgt cggacgtgcc ggcggtcttc tggggcacgc tgggggtcta
      961 cttcagcctg agggcctggc cgccagggga aaggcccagg gcacgccgct ccgagtggcg
     1021 ctggggcctg gcggcagggg cggccctcgg tgtggccgtg ctggtgcgct atagctccgc
     1081 gctattgctc ccagccctgg tggcgtatct cgcggcgcgg ggctacatgc agaggaggga
     1141 gtcgcgtacg ccacgggagg agtcgcctcc cgcattgtgg cggtcgagct ttgcttgggc
     1201 tgccgtgggc ctggccgtag ccttgctgcc gcaggtcgct tatttgctgg cgcacgagcc
     1261 tggcccggcg ctgagaggct ggtcgccagg caacattttc agcaacaccg tgacggggtc
     1321 cgacggcacc acgagcttct ccacgccaat gataggcttc tacttgctgt ccgtgctggg
     1381 caacgttggt ggaggattcc tgtcgccctt ctgcctgccc gcgttgctgg ccggagtgtg
     1441 gatactggta cgtcaggcca acatttctgc tacaattctt ttgtttataa ggtggttgat
     1501 tggtatattg gcttatagtg gcacaatgta tcagacgcac agattcacgc tgtccaaaat
     1561 gcccgctctt gctgtgctga tcggtatcgg tgcgggtgct gctttcacgc agctacaaat
     1621 aggagcgcaa gaccggcggg aaagggccgg taacgggcaa gcggtaaggg ggcttatgtc
     1681 ggcacttctg ctcgttgggc tgggggtcgg gctgtggcag ggagagcgct ccgccgagcg
     1741 gtgggtagcc acccacgctg agttcaacgc ccaggagcag cgggtggtgg ccctcgcgcg
     1801 ggaggcggct gcggcctcgg agcaggcggg cactcccagg gtggtgtgct tcggcttcag
     1861 cgcccccctc taccactaca cccattggcc catactcgat ttcttcgccc acgacgaggc
     1921 cgacatcgag ggcttccttt ccgcacccgg cccccacctg ctggtgctgc cggaggagag
     1981 catgtcaact cagtgggccg ggacgcctcc cggcgcgcgc tgggagtgga tcaagagcac
     2041 ctaccggctc accaaacagg ggcaggcagg ggtgttcacc gtgtacgcca tcgggggccg
     2101 ccaggcggat caaggggtgc cttcttccag ccccagcagc agcgacgctt cgggcgtgcc
     2161 cactaccccg gccaactgca tcgcctggcg cagctccttc gactccatga agccgcgggc
     2221 ctgctctacc gtggcaaatt cctccatcac cagcaggtcg ttgcggtcgc cggccacctg
     2281 gaagaggcgg tagcgtttcc acccgtagcg catcttcaac tccgagaggc catcgaagac
     2341 ctctttccac ctgttgtagt cccctaccct gtgtctgatg tgtatccagg ccatgcaatt
     2401 tgctcctttg cggctgtcag tgtcgagacc ggcagaacga cacaacagca cagaccgcgc
     2461 ccggcttgga agtgccggta tggtaaaatg cctggacgcc acctcaagag cgacggacta
     2521 gaatataagc aactaagccc taatctttgt catttcgaac gcagtgagaa atctcgtcct
     2581 acagcaccca ggtaacacct ttcttacagg actgagccac atgagaaaag agcaacatcc
     2641 cggtgaggga cgagattctt cgctgcgctc agaatgacaa aagtggggct tggggactga
     2701 cacccaacag tcttgagatg gccacgagct atcgttgttc ttgaataggt attggctcac
     2761 gaaaccatgc gaggctagaa agtaagcaat gagcaagttg ttacgattct ctacatttct
     2821 cgcgccgagc atcaggcccg tttatgaggc cgtagccggg tacgttgggc gggagcttgg
     2881 ttgtgacacc gcgctcgtgg aaggccgctc atttggcgag ttcgcggagg gcgcgtgcga
     2941 tatcgggttc atttgcgggc tgccttacgt gcaactcgcg gcacaagacc cgcgccccgt
     3001 ggagctactg gccgccccgg tgctgcaagg ggagcgttat agcggtaggc ccatctattt
     3061 ctccgatgtg atcgtgcgga gcgacgcccc ttttcacagc ttcgcggacc tgcgtgggcg
     3121 tagctggtct tacaacgacc ccgattcgca ttcgggctat ggcgttacgc gctaccacct
     3181 cgtgcgcctg ggagagaccc ggggctactt tgggagcgta gtagcggcgg gcttccacca
     3241 ggagtcgata agaatggtcc gcgagggcaa gatagacgcc tcagccatag attcgcaagt
     3301 gcttgcggtc gccatgcgcg acgatgcgga gcttgcgcgg caagtacggg tgattggcag
     3361 gcttggcccc tcgagcatac agccggtagt ggccgcgcgc acgctccccg acagcctcaa
     3421 ggccgacgtg caggccgcca tcttacggat gcacacggac gctgaagcgg tgagggggtt
     3481 gtcgcacggc ctcgtagagc ggtttgcgcc tgtggccgac gcggactacg acgatatacg
     3541 cgagatgctt gcagcaaccg aggcggtggg cttcggggtg ctcaggtagc cgcgccacgc
     3601 ggggaggacc ggcacaaaca tgcgacccaa agtaacggtg atttcgctgg gcggcactat
     3661 cgccatgacc acgacggacg acggaggggt cacgcctacg ctggacggcg cggcgttggt
     3721 gtcggcggtg ccgggcctcg gtgaggtcgc ggagctgacg gctgtgtcgt tccggcagct
     3781 tccgagcgcg cacctgggcc tcgaggacgt gctggctctc gcctccgaag tggcacgaca
     3841 catcgacgcc ggcgcgggcg gcgtggtcat cacgcagggg accgacacgc ttgaggaggt
     3901 agccttcgcc ctcgacctgc tgctggaccg cgaagccccg gtggtagtca cgggggccat
     3961 gcgcaatccc accctcgcaa gcgcggacgg ccccgccaac ctgctggcag ccgtacgggt
     4021 ggcggcgagc agccaggcgc ggggcctcgg cgtgttggtc gtgctcaacg atgaggtaca
     4081 cgcggcccgc tacgtacaaa agacgcacac caccggcacg gcggccttcc gctcgcccct
     4141 gaccggcccg ctggggtggg tggtggaggg ccgcgtgcgc gtaaacaaca ggccggtagc
     4201 ccgcccttgc gttgaatggg aacctggcgg cgatgttgca ccagtggtgc ccgtggccct
     4261 cctgacggtg agcctgggcg acgacgggcg cctggtagac ctggtgcggc aggcgggtta
     4321 cggggggctg gtgattgagg cattcggggc tgggcacgtg cacgtgccgg tggcgtacag
     4381 gctgaaggaa ttggcaggcg tgatgcccgt ggtgctggcc tcacgcacgc gctccggcga
     4441 catgctgcgt cacacctacg ccttcagagg ctcggagacg gacctgctct ccagcgggct
     4501 gataagcgcc gggccgctcg atggcccaaa ggcgcgcgtc ctgctctcac tactcctgac
     4561 agcaggtgcc aaccgggagc aagtcgcggg tacgttcgca gcctgggcgg gcgactagcg
     4621 gcccc
//
LOCUS       DASRWZ010000416         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_514734, whole genome shotgun sequence.
ACCESSION   DASRWZ010000416 DASRWZ010000000
VERSION     DASRWZ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514734"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFH60_06445"
     CDS             complement(<1..628)
                     /locus_tag="VFH60_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313456.1"
                     /translation="MMSNKATRLVTLALALLTLLTTTTYSAAPASNTPAPSRPRGIYN
                     TLAQQPDSRVFPETGREVKGRFLKYWQEHGGLAQQGFPLSNEMQERSEVDGKTYTVQY
                     FERAVFELHPENQPPYDVLLSLLGSLQYAQKYPQAAPNQTPNTAEGSVLFPETGKRLG
                     GPFLAYWQAHGGLPQQGYPISDEFKEVSPLDGKTYTVQYFERAVFELHP"
     gene            934..1539
                     /locus_tag="VFH60_06450"
     CDS             934..1539
                     /locus_tag="VFH60_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313457.1"
                     /translation="MRKIGLVISVASILAICLWRPPEALATSCGGPFTLQEAVEASDV
                     VFFGEIREITEEVGDLYRTVVTYDVLSTWKGPIVSQQAVYRLERTESTTEDLLGKRHI
                     VYAGRHEDGRLSTSICIYIRPREQWYANELNLLGPGTPLVPEEAIAAYERELADVLSR
                     PDSGEWVPYLLAASGLVAVIVVLVAIARHRKQRLRISGDEV"
     gene            complement(1540..>3308)
                     /gene="priA"
                     /locus_tag="VFH60_06455"
     CDS             complement(1540..>3308)
                     /gene="priA"
                     /locus_tag="VFH60_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET6313458.1"
                     /translation="TRNDEPPPLTPAQAAAWREIAKAISNLDFRTSDLPEEEQIQNPK
                     LALERGEGSKSQNPKFLLHGVTGSGKTEIYLRAIGLALRLGRQAIVLVPEISLTPQAV
                     HRFAARFPGRVALIHSQLSPGQQFDEWRRIREGHADIVVGSRSAVFAPLPRLGLVVVD
                     EEHEWAYKQQDHQPRYHARDVALRRAELAGAAAILGSATPDLVSYYHANKGDYTLLTL
                     PTRVGRRRTRAGGEISTELPMPSVQVVDMRAELRAGNSSLFSRTLQTVLQSTLARREQ
                     AILYLNRRGSSSFIVCRECGYVPLCPRCDVPLVYHADIYGMLCHRCNAFSLAPRECPK
                     CAGSQIKGFGTGTQKVVDEVSALFPDARVLRWDRDTASRHGSHAGLMEMFAQGEADIL
                     VGTQMIAKGLDIDKVSLVGVISADTGLFLPDFRAPERTVQLLMQVAGRAGRRTETTHS
                     RVVVQTFNPDNYAIQAAAEYDYRGFYSGEIRFRAEHGYPPYGQVARLVYSSPSNERCE
                     QEANTLARHLRRTVEELKQALNDSPAADVDVLGPAPCFVHKVSGRYRWQVLLRGTDLS
                     LALDSFYPGPGWSLDVDPMSLL"
BASE COUNT          585 a          891 c         1180 g          652 t
ORIGIN      
        1 cagggtgcaa ctcgaacacg gcccgctcga agtactgcac agtataagtc ttaccgtcga
       61 ggggggacac ttccttgaac tcgtcggaga ttgggtagcc ctgctgcgga aggccgccgt
      121 gggcctgcca gtaagccagg aaaggccctc ccagccgctt gccggtttcg gggaagagca
      181 ccgacccctc ggcggtgttg ggcgtctggt tgggcgcggc ctgggggtac ttctgcgcgt
      241 actgaagcga acctaagagg gagaggagca cgtcgtaggg cggctggttc tcggggtgta
      301 actcgaacac ggcccgctcg aagtactgta ccgtgtaggt cttgccgtct acctcggaac
      361 gctcctgcat ctcgttggag agcgggaaac cttgctgggc gaggccgccg tgttcctgcc
      421 agtactttag gaacctgccc ttcacctcgc gcccggtctc agggaagacg cggctgtcgg
      481 gttgttgggc gagggtgttg tagatgcctc gtggccgaga aggggccggt gtgttacttg
      541 ccggggcggc agagtaggtt gtggtggtta gtagcgtgag caatgccagg gctagcgtca
      601 ccagtcttgt ggccttattg gacatcatgt ttacctcacc tccgtcggcc gactatattc
      661 agtaacttac agggtccgtt tgactgcacc ggtcgctgct tgtatgttgg ggcggacata
      721 cctgcccctt tttgagcagc ctcgttcctt aagttaatta ctcacctgtt gaaacaattg
      781 ttgggtactt gtcgtctgtt atagaaccgt ctgagtgaga gcaaagcgcc cgtgggattt
      841 gctggctcaa ctacaatagc tcccaggcga tacatagcca gcccatgtag catagaatag
      901 ctatagtggt tgtggggaag gggaggttgt acgatgcgga agataggtct cgtcattagt
      961 gtagcgagca tcctggctat atgcctctgg cggcctccgg aggctcttgc tacctcatgt
     1021 ggggggcctt ttacccttca ggaagccgtc gaggcatctg atgttgtgtt ctttggggag
     1081 atcagggaga ttaccgagga agttggcgat ttgtatagga ccgtggtgac ttacgacgta
     1141 ctttctacgt ggaagggtcc tatcgtctcg caacaggcag tgtataggct ggagcggact
     1201 gaaagtacga cagaagacct cctggggaag aggcacatcg tatatgccgg gaggcacgaa
     1261 gatggaagat tgagcacatc aatatgcata tacattaggc cacgggagca atggtatgca
     1321 aatgagctca acctactagg accgggtacc ccgctcgtgc ctgaagaagc gattgcagct
     1381 tacgagcgag aactggcaga tgtcctctcc agacctgaca gcggcgaatg ggtgccttac
     1441 ctcctggcag cgtccggcct ggtcgccgta atcgttgtcc tggtggctat cgcaaggcac
     1501 aggaaacagc gcctccgcat atctggagac gaggtgtagc tacagcaggc tcatggggtc
     1561 cacgtcgagg gaccagccgg ggcctgggta gaagctatcg agggccaggc tgaggtcggt
     1621 gccgcgcagg aggacttgcc agcggtagcg gccgctgact ttgtggacga agcagggggc
     1681 ggggcctagc acgtctacgt cggcggcggg ggagtcgttg agggcctgct ttagctcctc
     1741 gacggtgcgg cgcaaatgcc gggccagggt gttggcttcc tgctcgcagc gctcgttgga
     1801 gggcgaggag tagacgaggc gggcgacctg gccgtagggc ggatagccgt gctcggcgcg
     1861 gaagcggatt tcgccggagt agaagccccg gtagtcgtac tcggcggcgg cctgaatcgc
     1921 gtagttgtcg gggttgaagg tctgcacgac cacgcggctg tgggtggtct cggtgcggcg
     1981 gcccgcgcgc cccgcgacct gcatgagcaa ctgcaccgtg cgctcggggg cgcggaagtc
     2041 gggcaggaag agaccggtat cggcgctgat taccccgacg agggacacct tatcgatgtc
     2101 gaggcccttg gcgatcatct gggtgcccac caggatgtcg gcctcgccct gggcgaacat
     2161 ctccatcagc ccggcgtgac tgccgtgccg ggaggcggta tcgcggtccc agcggagcac
     2221 gcgcgcgtcg gggaagagag ccgatacctc gtccacgacc ttctgggtgc cggtgccgaa
     2281 gcccttgatc tggctgccgg cgcacttggg gcactctcta ggcgcgaggg agaaggcgtt
     2341 gcagcggtgg cagagcatgc catagatgtc ggcgtggtac acgaggggca cgtcgcagcg
     2401 tgggcagaga gggacgtagc cgcactcgcg gcagacgatg aagctgctgc tgccgcgcct
     2461 gttgaggtag aggatggcct gctcccggcg ggcgagggtc gattgcagga cggtttgcag
     2521 ggtgcggctg aacagggagg agttaccggc gcgcaactcg gcccgcatgt ccaccacctg
     2581 cacggagggc atgggcaatt cggtagagat ctcgcccccg gcgcgggtgc ggcggcggcc
     2641 cacgcgggtg gggagggtga gcagtgtgta gtcgcccttg ttggcgtggt agtagctcac
     2701 gaggtcgggg gtggcgctgc ccaggatggc ggcggccccg gcaagctcgg cgcgcctgag
     2761 ggccacgtcg cgggcgtggt agcggggctg gtggtcctgc tgcttgtagg cccactcatg
     2821 ctcctcgtcc accacgacaa ggccgaggcg ggggagcggg gcgaacacgg cggagcggga
     2881 gcctaccacg atatcggcgt gcccctcgcg gatgcgccgc cactcatcga actgctggcc
     2941 gggggaaagc tggctgtgga tgagtgccac cctgccgggg aagcgggcgg cgaagcggtg
     3001 gaccgcctgg ggcgtgagcg aaatctccgg cacgagcacg atagcctgcc tgcccaaccg
     3061 caacgccagc ccaatggccc gcaggtaaat ctcggtcttg ccggagccgg tgacaccgtg
     3121 gaggaggaat tttggatttt ggcttttgga cccttcgcct cgctcgaggg ctagctttgg
     3181 attttggatt tgttcttctt ccggtaaatc cgaagtccta aagtccaaat tcgaaatcgc
     3241 tttcgctatt tctcgccagg cggcggcctg agcgggggtg aggggcgggg gttcgtcgtt
     3301 gcgagtgg
//
LOCUS       DASRWZ010000417         5292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_788963, whole genome shotgun sequence.
ACCESSION   DASRWZ010000417 DASRWZ010000000
VERSION     DASRWZ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788963"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1259)
                     /locus_tag="VFH60_06460"
     CDS             complement(216..1259)
                     /locus_tag="VFH60_06460"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HET6313459.1"
                     /translation="MKKLNFLLLIAALSLVLSACDAGNSAAPTAVPAGTPVATNVPPT
                     GTTGALQPTTEVGQPTATSRASTEPLPTGTVQPDPTAVSTPEAVDVTPNETSVPDDAN
                     IAVKPNTGTGIDFPADGAQVTFPIHVQARVGRLNNEVVLKIKWQDGREYSEPYQVQFD
                     KEGNGLLVDSLDWTGESAPPEWPSQPATLQVLAPSGTVLASRKVNYLGATDPNTMQIK
                     VYWLLDDGPRDIPQRIVRTRQVGAAALSELLWGIGPRNFAGFTTAIPSPEEVLNYAGR
                     QPDWGPRVRLRKLTIVDGVATVDFSKEMAAYGGGSARVNAIEEQVTLTLKQFPSVKEV
                     RILVDGKPDALQP"
     gene            complement(1339..5019)
                     /gene="smc"
                     /locus_tag="VFH60_06465"
     CDS             complement(1339..5019)
                     /gene="smc"
                     /locus_tag="VFH60_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET6313460.1"
                     /translation="MRLKHLTLQGFKTFVPKTDLVLGEGITAIVGPNGSGKSNVADAV
                     RWALGEQSLSMLRAKRTEDLIFAGSSNRQPVGMAEVSLTIDNSDRLLPLDFAEVTLTR
                     RAYRSGENEYYINKSRVRLRDVLDVASTLGQAYTVVGQGLIDAALSLRPEERRELFEE
                     AAAIRGYFVQREDALRRLTRTEENMNRVADLIAELEPQVRRMERQARQAQEYGKIEAD
                     LREAMQEWYAARWLIACAELDRAQANDGTARREVESRKAEVAAKLAELVEARRSVWEL
                     VDAVSALHEQRAQIQARHATQSQAQAVLAERLSASRSQKRQVLQEQAGLASAIQQARE
                     QLEAAGQDVEAREAEIVTLREAGKEIATTLAGWDARIGELRARHEREAGALEGTARQG
                     AELRSRMSQAQRQISEQESVAHEAESRLGTLAQKLASEETALEERHARLMAAQKAVSS
                     VEAAQSAAQSGLNKARAAQSAAEAARRELQRRLDGVGSQIAALAGEQQASLYGGVRAV
                     VGAAREGRLSGYVGTVAELLQVPTDLETAIEAALGGRLQDVVVSTWADAERAIALLKQ
                     SGAGRATFLPLDTLRATPASNPPAGAGIVGLARDLVEYEPHLKTLAESLLGRLLILED
                     LPSARRTIASLPANAPWTLATLGGEVVRPGGSVTGGSNSRADDNRARGRTILARERKQ
                     RELGLAQQELKQELAGAERALAASIESVREQEAALSSLAGQSEDARRRQVAAQMAHVE
                     QQSAVARLQQEIAWRTGLLAETRRTSETARGVAEHTGAEIEQLEVGLEPRRARLAEVE
                     TQLKQALSERESAAGLVASEQTRLAVLEEGLRNARARQADLWQDLSRYQGREADFAAR
                     LQAADRDETALTGQIDEHAAHVAELAEQLARVEAQVDPSEHEVRVLEAEVGKVESEQA
                     VLQSLLLESETALSHAAVERQRCIGVLDSLQVEISEELGEAHAGHEAVGHLVVAGAAM
                     GGEEAYTGMQVVWPSALRRPDPPPAGGPANGHATAIVEDMQPLERRVYALKARLSRIG
                     PVNPLAMEEFTSLSERHAYLQTQLGDLSTAGESLRRVIAELDRVMRDQFATTFEQVNE
                     KFQHFFSLLFAGGTARLELTNPQDAGQSGIEISAQPPGKRMQPLAALSGGERALTAAA
                     LLFALLKVRPVPFCVLDEVDAALDESNVTRFRSALQELGDKTQFVIITHNRGTIEAAN
                     TLYGVSMAGDGTSQLLSLKVED"
BASE COUNT          812 a         1799 c         1660 g         1021 t
ORIGIN      
        1 aggttgttcg ttgtaccact agccactccg tacccgctat ctaaacgcca agacgccaag
       61 gaccacgcca agattatgga tgtagtgttc taagcaatag gaccagggta ctgatgttgt
      121 accctggtcc cttcttgtac tctccgcacc tcatttactt ggcgtcttgg cgtttagaaa
      181 ccctgtatct tcagcttgcg gtgacgcaca tagggctagg gctggagggc gtccggcttg
      241 ccgtcaacca gtatgcggac ctccttgacg gaggggaact gtttgagggt gagagtcacc
      301 tgctcctcaa ttgcgttcac gcgcgccgag ccgccgccgt atgccgccat ctccttcgag
      361 aagtccaccg tagccacgcc atcgacgatg gtcagctttc gcagacgcac gcgcggcccc
      421 cagtcgggct ggcgaccggc ataattgagc acttcctccg gcgatggtat agccgtggtg
      481 aagccggcga agttgcgcgg gccgataccc cacaaaagct cggaaagggc ggcggcacct
      541 acctgccggg tgcggactat ccgctggggg atgtcgcgtg ggccgtcatc gagcagccag
      601 tagaccttta tctgcatggt attcgggtct gtggcaccca ggtaatttac cttgcggctg
      661 gcgagcactg taccggaagg ggcaagcact tgcaaggtgg cgggctgaga cggccactcc
      721 gggggtgcgc tttcgccggt ccagtcgagg ctgtccacga gcaggccgtt gccctccttg
      781 tcgaactgca cctggtacgg ctccgagtac tctcgtccgt cctgccactt gatcttgagc
      841 acgacctcgt tgttgagcct gcccacgcgg gcctggacgt gtatcgggaa tgtgacctgc
      901 gcgccgtccg ccggaaagtc gatgccagta ccggtgttgg gcttaacggc gatattcgcg
      961 tcatcgggta cgctcgtctc gtttggcgta acgtccaccg cctccggggt gctcaccgcg
     1021 gtcgggtcgg gctggaccgt gcccgttggc agcggctcgg tggaggcccg gcttgtggcc
     1081 gtgggctgtc cgacttcggt cgtcggctgt agcgcaccgg tagtgccggt tgggggaacg
     1141 tttgtagcga cgggcgtacc agccggcaca gcagtaggtg ccgccgagtt acccgcgtcg
     1201 caggcagaga gcacaagact gagggctgca attagcagca agaagttaag cttcttcatg
     1261 atgtatacct ttgagaaaat tgatagtggt cgcccaatat tagaacgtct ggggcgtggt
     1321 cgaagttccg ctggcgagct aatcttccac cttgagcgag agaagttgcg aagtcccgtc
     1381 gccggccatc gatacgccgt acagagtatt cgcggcctct atcgtgcccc tgttgtgggt
     1441 gatgatgacg aactgggtct tgtcgccaag ctcttgcaga gccgagcgga agcgggtgac
     1501 gttgctctcg tcgagggccg cgtccacctc gtccagcacg cagaacggca ccggccgcac
     1561 cttcagcagc gcgaacagga gggccgcggc ggttagcgcc cgctctccac ccgaaagggc
     1621 ggccagcggc tgcatgcgct tgccgggcgg ctgtgccgaa atctctatac ccgactgccc
     1681 ggcgtcctgg gggttggtaa gctctagccg cgccgtgccc cccgcgaaaa ggaggctgaa
     1741 gaagtgctgg aacttctcgt tgacctgctc gaaagttgtg gcaaactggt cgcgcatgac
     1801 ccggtccagt tcggcaataa cccgccgcaa cgactccccg gccgtgctca ggtcgccaag
     1861 ttgcgtttgc aggtacgcat gccgctctga tagcgacgtg aactcctcca tcgccagcgg
     1921 gttcaccggg ccgatgcgag acagccttgc cttcagggcg tagaccctcc gctccagggg
     1981 ttgcatgtcc tccacgattg ctgtcgcgtg cccgttggcg gggccaccgg cgggcggcgg
     2041 gtccgggcgg cgcagtgccg agggccacac cacctgcatg cccgtgtagg cctcctcgcc
     2101 tcccattgcc gccccggcaa ccactaggtg acctactgcc tcatgtccgg cgtgggcctc
     2161 acctaattct tcgctgatct ccacctgcaa gctgtcgagc acgcctatgc accgttgcct
     2221 ttccacggca gcgtggctca gtgcggtctc gctttcgagc agcagcgatt ggaggactgc
     2281 ctgttcgctc tcgaccttac ccacttccgc ttccagcact cgtacttcat gctcggatgg
     2341 gtccacctgc gcctctaccc gcgcaagttg ctccgctagt tcggctacgt gcgcggcgtg
     2401 ctcgtctatc tggccggtga gggcggtctc gtcccggtcc gccgcctgca accgcgccgc
     2461 aaagtctgcc tctctaccct ggtagcggga caggtcttgc cacaggtcgg cttggcgggc
     2521 gcgggcgttg cgcaagccct cttcgagcac cgccaggcgc gtctgctcgc tcgccaccag
     2581 cccagccgcc gactcgcgct cgctgagggc ctgcttgagt tgggtctcca cttccgccag
     2641 gcgcgctcgc cgtggctcaa gtcccacctc tagctgctcg atctccgcgc ccgtatgctc
     2701 cgcgacacct cgcgcggtct cactcgtgcg gcgggtctcg gccagcaacc cggtgcgcca
     2761 ggctatctcc tgttgcagcc ttgcgaccgc cgactgttgc tccacgtggg ccatctgggc
     2821 cgctacctgc ctcctgcggg cgtcctccga ctgccccgcc aggctggaaa gcgcggcttc
     2881 ctgctctcgc accgattcga tggaggctgc cagcgcgcgc tcagccccgg caagctcctg
     2941 cttcagttcc tgctgagcaa ggccaagctc ccgctgctta cgctcgcggg ccaggatggt
     3001 gcggcccctg gcgcggttgt cgtccgcccg gctgttcgag ccacccgtta cagagccgcc
     3061 ggggcgcacc acctccccac ccaacgtagc cagcgtccag ggcgcgttgg cgggcaacga
     3121 ggcaatcgtt cggcgggccg agggcaggtc ctccaggatg agcagcctgc ccagcagcga
     3181 ttcggccagc gtcttgagat gcggctcata ctctacaagg tcgcgggcaa ggcccactat
     3241 cccggcccct gccggtgggt tagaggcagg tgtagcccgc agggtgtcga gcggaaggaa
     3301 tgtagcccgg cccgcgcccg actgcttgag cagggcaatg gcacgctcgg cgtcggccca
     3361 ggtggacacc accacgtcct gcaagcggcc gcccagcgcc gcctctatcg cggtctcaag
     3421 gtccgtaggc acctggagca gctcggccac cgtgccgaca tagccactca agcggccctc
     3481 acgcgctgcg ccgaccaccg cccgcactcc accgtaaaga ctggcctgct gctcccccgc
     3541 cagcgcggcg atttgcgacc caacaccgtc cagccgccgc tgtagctctc tgcgggcggc
     3601 ttcggcagcc gactgcgcgg cgcgggcctt gttcagtccc gactgcgcgg ctgactgcgc
     3661 ggcctcgacg ctggagacgg ccttctgcgc cgccatgagc cgcgcgtgcc tctcttccag
     3721 ggcggtttcc tctgacgcca gcttctgcgc gagcgttccc agcctgcttt cggcctcgtg
     3781 ggctacgctc tcctgctccg aaatctgccg ctgggcctgg gacatacgcg acctcagctc
     3841 cgcgccctgc ctggccgtgc cctccagcgc cccggcttca cgttcgtggc gggcgcgcaa
     3901 ctcccctatc cgggcgtccc accccgcgag cgtagttgct atctctttcc cagcctcgcg
     3961 cagcgtgacg atctcggcct cacgggcctc aacgtcctgt cccgccgcct ccagttgctc
     4021 ccgggcctgc tgtatggccg aggcaaggcc cgcctgctcc tggaggacct ggcgcttctg
     4081 cgaccgggag gccgatagcc gttcagccaa taccgcctgt gcctgcgact gtgtagcgtg
     4141 gcgggcttgt atctgcgccc gttgctcgtg tagcgccgac acggcatcca ccagttccca
     4201 gaccgaccgt cgggcttcga ctagctccgc caacttcgcc gccacctcgg ccttgcggct
     4261 ctcgacctcg cgcctggcgg tgccgtcgtt ggcctgggcg cggtccagtt cggcgcacgc
     4321 gatgagccac cgggccgcgt accattcctg catcgcctcg cgcaggtcag cctcgatctt
     4381 gccgtactcc tgggcctgtc gtgcctgccg ttccatgcgc cgtacctggg gctctagctc
     4441 ggcaatgagg tcagccaccc gattcatgtt ctcctcggtg cgggtgaggc ggcgcagggc
     4501 gtcctcccgc tgcacgaagt agccccgtat ggcggcggct tcctcgaaca gctcccggcg
     4561 ttcctcgggc cgcaacgaca gggccgcgtc aatcagcccc tggcccacca cggtgtacgc
     4621 ctgccccagc gtcgaagcta catcgagcac gtcgcgcagg cgcacgcggc tcttgttgat
     4681 gtagtactcg ttctcgccgg aacggtacgc gcggcgggtc agggtgacct ccgcgaaatc
     4741 gagcggaagc agacggtccg agttgtcgat ggtgagggat acttcggcca tgccgaccgg
     4801 ctggcggttg gaggaaccgg cgaaaatcag gtcctcggtc cgcttggcgc gcaacatgga
     4861 gagggactgc tcgcccagcg cccaccgtac cgcatccgcg acgttgctct tgccgctgcc
     4921 gttcgggccg acgatggccg tgatgccttc gcccaggacc aggtcggtct tcggaacgaa
     4981 agtcttgaat ccttgcagcg tgaggtgttt gagacgcatg gggaagtggt gggatttggc
     5041 ccggcaagcc ggtggcatgg ggccggtggt tacgtagtaa gccagcgccc gacatagaag
     5101 gtaccggcaa gctaccgggc gggcacaaag gtcattgtac cacagggcgg cagtaagtgt
     5161 gaactgaccg gtagcgagtt tgacacagca cgaagctacg agtaaacttt ctcactaaga
     5221 cctgaccgta attgcccact ctaagtgcga tgatatgcac acattgtgct atctatccct
     5281 tgcatgcctg ct
//
LOCUS       DASRWZ010000418         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_446139, whole genome shotgun sequence.
ACCESSION   DASRWZ010000418 DASRWZ010000000
VERSION     DASRWZ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446139"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1855)
                     /locus_tag="VFH60_06470"
     CDS             complement(11..1855)
                     /locus_tag="VFH60_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313461.1"
                     /translation="MAVREAGKARMSTVRLCLWLLRYPLRRWSWLVAAVVTMLLKVGL
                     DLLKPWPMKVLVDQVLSNKPVPGSMTSLFGWLPGGLTRDSLLTWSVLATVIIFLVAWA
                     LGVVSAYVDIGFGRRMTYDLAGDLFGHLQRLSLRFHRRKSTGDTIRRVTTDCASVSTI
                     VKNALLPAAVAIVNFAAIFAVMWALDPVLMLLSLAAVPPMALTFRRYAGRMLERSYRQ
                     QEAEGEVYNVVEQTLSSMPIVQAFGGEERADRRFQEGTGATLEATVATTEVEFRFKML
                     IGLATALGTAAIIWVGGQHVLDGSLTVGSILLFISYLGLLYGPLESVMYMSSTVQSAA
                     GSARRVLEVLETEQDVESSPGARPLPPVEGHVIIENVTFGYEPGRPVLEEVYLEALPG
                     ETVAIVGPTGAGKSTLVSLVARFFDPWEGTVWIDGYDARAVQLRSLRNQVAVVPQDPF
                     LFPTTVAENIAYGRPEASRKKVKAAARAANAHPFIERLPDGYDTKLGERGGTLSGGER
                     QRLAIARALLKDAPILVLDEPTSALDSETEGLVMEAMRRLMRGRTTFIIAHRLSTIRN
                     ADKIAVLKDCRIVEVGTHEELLARRGVYARLYGYQRGPQLEVAREVVK"
     gene            complement(1845..2591)
                     /locus_tag="VFH60_06475"
     CDS             complement(1845..2591)
                     /locus_tag="VFH60_06475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6313462.1"
                     /translation="MEQAGPESSILAVLGFHKVGAPPPGGWDTWFYVPEATFVGYLTY
                     LREHGWEAISVESLLRGLAEPGSLPARAVLITFDDGYRSLLEVALPHLLRFGYPGTLF
                     VPTDFIGGSNLFDAGVEPEEAICDWADLRELTGLSISVQSHGAAHRPFSTLNPAEQRE
                     ELQRSKAVLEHGLGEAVEIFSYPYGDAGPDADATRRALAEAGYKAACLYGGGPNRLPA
                     SDPDRLTRLAMGPDTDLHALLGEFGGSDGG"
     gene            complement(2599..>3095)
                     /locus_tag="VFH60_06480"
     CDS             complement(2599..>3095)
                     /locus_tag="VFH60_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313463.1"
                     /translation="PNDKRDGFLPFLGLPCRTAQPLELALYLEGDQTERRALEARGWR
                     VRESVEVAATPCDYQGYIQASRGEFSCVKPSCVRMQNAWISDRTLCYLASGKPAVVQH
                     TGTSRFLPDAAGLFRFSDLGEAARCLDAVASDYERHGRLARALAEEHFDARKVVGSVL
                     ERAL"
BASE COUNT          583 a         1083 c          895 g          534 t
ORIGIN      
        1 aggatgatat tcacttaaca acctcgcgcg caacctcaag ttggggccca cgctgatagc
       61 cgtaaaggcg agcatacacg cctctccgcg ccagcagttc ttcgtgcgtg ccgacctcga
      121 cgatccggca gtccttcagc acggctatct tgtcggcgtt gcggatagtg gagaggcggt
      181 gggcgatgat aaaggtagtc ctcccccgca tcaaccggcg catcgcctcc atcaccagcc
      241 cttctgtttc cgagtccaga gcactcgtcg gctcgtccag gacgagtatg ggcgcatcct
      301 tgagcagcgc ccgcgcgata gccagtcgct gccgctcgcc acccgacagc gtgcctccac
      361 gctcccccaa cttcgtgtcg tacccgtccg gcaacctctc aataaaaggg tgggcgttag
      421 ccgcgcgagc cgctgccttg accttcttcc ttgaggcttc cgggcggcca taagctatgt
      481 tctccgccac cgttgttggg aacaagaaag ggtcttgggg cacgacggct acctggtttc
      541 gcaggctcct aagttgcacc gcccgcgcat catagccgtc aatccacacg gtcccctccc
      601 acgggtcgaa gaagcgcgcc accaggctca cgagggtgct cttgcccgcg cctgtcgggc
      661 cgactatggc gacggtctcg ccggggaggg cttccaggta cacttcttcc agcacggggc
      721 ggcccggctc gtacccgaag gttacgttct ctatgatgac gtgcccctct acaggcggca
      781 ggggccgcgc gcccggcgaa ctctccacgt cttgctcagt ctccagcact tccagcacgc
      841 gccgtgcgct cccggctgcg ctctgcacgg tggaggacat gtacatgacc gattcgagcg
      901 ggccgtacag caggccaagg taggagatga agagcaggat gctgcccacg gtcaggctcc
      961 cgtcgagaac gtgctgccca ccaacccaaa taatagcagc cgtgccgaga gcggttgcca
     1021 gcccaatcaa catcttgaag cgaaactcca cctctgtggt ggcaaccgta gcctcaaggg
     1081 tagcgcccgt gccttcctgg aagcggcggt cggcgcgctc ctctcccccg aaagcctgca
     1141 cgatgggcat agacgacagg gtctgctcca ccacattgta cacctcgcct tcagcctcct
     1201 gctgcctgta actccgctcc agcatgcgcc ccgcgtaccg ccggaacgtc agggccattg
     1261 ggggcacggc ggccagagaa aggagcatca acaccgggtc aagcgcccac ataacggcga
     1321 atatagccgc gaagttgacg atagcgacgg cggcaggcag gagcgcgttc ttgacgatgg
     1381 ttgacacgga agcgcaatcc gtcgtcactc tccttatcgt atcgcccgta gacttgcgcc
     1441 tgtggaagcg cagcgacagc ctctgcaagt gcccgaatag gtctcccgcc aggtcgtaag
     1501 tcatgcgccg cccgaagccg atgtctacat atgcgcttac cacgcccaac gcccaggcca
     1561 ccaggaagat tattacggtg gcgagcacgc tccacgtcag gaggctatct cgcgtgaggc
     1621 cccccggcag ccagccaaag agagatgtca tgctgccggg caccggctta ttgctcaaca
     1681 cctggtctac cagcactttc atcggccagg gtttcaggag gtccagcccc accttcagga
     1741 gcatagtaac cacagccgcc accagccacg accaccgccg aaggggatag cgcagcagcc
     1801 acaggcagag ccgcaccgtg ctcatgcggg ccttcccggc ttctctaacc gccatcactg
     1861 cccccgaact cgcccagcag ggcgtgcaaa tcggtgtccg gtcccatcgc cagcctggtc
     1921 aggcggtcgg ggtccgaggc gggcaggcgg tttggaccgc ccccgtacag gcatgcagcc
     1981 ttgtagcccg cctccgcgag cgccctgcgg gtggcgtcgg cgtccggccc ggcgtctccg
     2041 taggggtagg agaaaatctc gactgcctct cccaggccat gctccaacac agctttggac
     2101 ctctgaagct cctccctctg ctccgccggg ttgagcgtcg agaaagggcg gtgtgcggct
     2161 ccatgcgact gcacggatat gctcaggccg gtaagctctc gcaaatcagc ccaatcgcag
     2221 atagcttcct ccggctccac acccgcatcg aaaaggttgc tccccccgat aaagtctgtg
     2281 ggcacaaaga gcgttcccgg atagccgaag cgcagcagat gaggcagcgc aacctccagc
     2341 agcgacctgt acccatcgtc aaaggtaata agcacagcgc gcgcaggcaa gctgcccggc
     2401 tctgccaggc cgcgcagcaa gctctccacg ctgatagcct cccagccgtg ctcccgcaag
     2461 taagtcaggt agcccacaaa agtggcttcc ggcacgtaga accaggtgtc ccacccgcca
     2521 ggtggcggcg cgcccacttt gtggaagccc aggaccgcga gtatgctgct ttcaggacct
     2581 gcttgctcca tgtcctctct acaacgccct ctccaacacg ctcccgacga ccttgcgcgc
     2641 gtcgaagtgc tcttccgcca gggcgcgcgc cagcctgcca tgccgctcgt aatccgatgc
     2701 tacggcatcc aggcagcgag ccgcttcccc caggtcgctg aagcggaaga ggcccgccgc
     2761 gtcggggaga aagcgactcg tccccgtgtg ctggaccaca gcgggtttgc cgctcgccag
     2821 gtagcaaagt gtgcggtcgc tgatccaggc gttctgcatg cgcacgcacg aaggctttac
     2881 gcaactgaac tctccccgcg aggcctgtat atacccctgg tagtcgcacg gcgtagctgc
     2941 cacctccacc gactcgcgca cccgccagcc tcgcgcttca agcgccctgc gctccgtttg
     3001 gtccccctcc aggtatagcg ccagctccag gggttgcgcc gtccggcatg ggaggcccag
     3061 aaagggcagg aaaccgtccc tcttatcatt cgggt
//
LOCUS       DASRWZ010000419         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_651815, whole genome shotgun sequence.
ACCESSION   DASRWZ010000419 DASRWZ010000000
VERSION     DASRWZ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651815"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1396)
                     /locus_tag="VFH60_06485"
     CDS             complement(221..1396)
                     /locus_tag="VFH60_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6313464.1"
                     /translation="MKVFEVIAPVERIATDPSGDRPSEGMALCLSGGGYRAMLFNLGA
                     LWRLNECGYLPRLKRVSSVSGGSITAAALGLAWGRLDFDESGRARRFNEEVARPLRNL
                     AGKTIDVRAVLRGLLGPGTINEALARQYRKHLYGNKTLQHLPADTEGPRFVINATNVQ
                     SGALWRFSRPYAQDWRVGQLRNPDFEVALAVAASSALPPFLSPAVLHIPASAYSEVAG
                     TDLHYAPFTTRVVLTDGGVNDCLGLETSWKRFSTILVSDGGGKISPEKMPHKLWPRHT
                     LRVLAMMDDQRRSRRKMQVMSSFTRARDQIGHRYGAYWSSRSDIKEYGLPDSLECPLP
                     QTAKLADLPTRLSRLDARVQEQLVNWGYAVCDAALRRYVDPLLPAPHDFPYPSAGVG"
     gene            1793..>4197
                     /locus_tag="VFH60_06490"
     CDS             1793..>4197
                     /locus_tag="VFH60_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6313465.1"
                     /translation="MSNLSAFGYWLRQQRKEYDMSQEDLAERVGCAYETIRKIEVGSR
                     RPSKQMAELLGKTFGIPAAELPAFIEFARQDPNAPGTGSDAPGPAALANQPIREKQSY
                     LGTLPAQRTSFVGRKSEIEGIRALLLSSHVRVLTLTGAPGTGKTRLALKVASSVQAEF
                     SDGVYFVGLASLSDPDKVPGVIARALNIKDEPSRPALALVQEQLRERVVLLLLDNFEH
                     LTWAGSYVGELVEACPNIKILTTSRTPLNIYGEYQFFIRPMDLPGTREQLGAEELYQY
                     ESAALFVQRARSVNPEFRLTAGNAAAVADICRQLDGLPLAIELAAAYCKILPPHAIRQ
                     KLTSRLNLLTLGASDLPRRQQTLRGAISWSHDLLSAQQEALFRRVSVFVGGWTLEAVE
                     GVCNLPDEADVGTIQGVVALLDRSLLEREDGAGGQPRFSMLQTIREYAQEQLNKSSEV
                     QLMQRQHARYFLAFAESAEPHLKGPERDLWLQRLELEHDNFRTALTWCMHTPEAADLG
                     LRLAGALHWFWYFRGYLTEGRDWLEGTLARAGGLRHSAAGAKALDAAGHLALLQDDYS
                     VMLDHLAESVSTWRSVGDKQGLAYALADYGIATVYRYRGNSVDGSEALKEAIQLFREV
                     GDRWGLALALNWLGDATGLLRHPEDAVVAYKSESLDLYRQLDDKWGIAFQLSELGRLA
                     VWQGNYELARARLEESLRTGGFGDKWSVAHVLRSLGDIALFQHDRAQAVLLYEESLAL
                     YRELGDKFRGSTCLRNLAGISLSEGETERAAQLYQESLQLAFALQNVPNVALCLAGMA
                     GVAL"
BASE COUNT          806 a         1289 c         1333 g          769 t
ORIGIN      
        1 agagtcctca aaattgggga tgatggcggc tagaggaaca ctattgccca tgaaggcaga
       61 gtcaagaacg ggcggctgat cctacgctgc gctcaggatg acagggggga atagggcact
      121 acaaactgcc accgcgcagg ccgcagacgc tcaaggtccc ggcggtggat atggggcgcg
      181 cgagctgagc ttgaggagac cagcaagccc gtggcggcgg ttacccgacc ccggccgaag
      241 gataagggaa gtcatggggg gcgggcagaa gggggtctac atagcggcgc aaggcggcat
      301 cgcatacggc gtaaccccag ttgaccagtt gctcctgtac ccttgcgtcc agcctgctca
      361 agcgggtcgg caggtccgcg agcttggcgg tctgggggag gggacattcg agggagtcgg
      421 gcagaccgta ctctttgatg tcagagcgac tgctccagta agcgccgtag cggtggccta
      481 tctggtcgcg cgccctcgta aacgacgaca tgacctgcat cttgcgcctg ctacgtctct
      541 ggtcgtccat catcgcgagt acgcgcaagg tgtgcctggg ccagagcttg tgcggcatct
      601 tttcggggct tatcttaccc ccgccatcgc tcaccagtat ggttgagaaa cgcttccagg
      661 aggtttccag cccgaggcaa tcgtttactc caccatccgt caggaccacc ctggtggtga
      721 agggcgcgta gtggagatcg gtgcccgcga cctcagaata ggccgaggcg gggatgtgca
      781 gcactgcggg cgagaggaaa ggtgggaggg ccgaagaggc cgccacggcc agcgccacct
      841 cgaagtcggg gtttcgcaac tggcccactc tccagtcctg ggcgtagggc cgggagaacc
      901 tccagagcgc gcccgactgc acgttggtgg cattgatgac aaatctcggc ccctcggtat
      961 cagcggggag atgttgcagg gtcttattac cgtacaggtg cttgcggtac tgccttgcca
     1021 gcgcctcgtt tatcgttccc ggcccgagga ggccccggag aacggcccgt acgtcaatcg
     1081 tctttccggc caggttcctc aggggtcgag ctacttcctc attgaagcgc ctggccctgc
     1141 cgctttcatc gaagtccagc ctcccccagg cgaggcccag cgccgctgcc gtaatggagc
     1201 cgcccgaaac gctcgatact ctcttcaggc ggggcaagta gccgcactcg ttcaagcgcc
     1261 acagcgcgcc caggttgaag agcatcgccc ggtagccacc acccgacagg caaagcgcca
     1321 tgccctcgct gggcctgtct ccactcggat cggtagctat acgctctacg ggcgcaatga
     1381 cttcaaagac tttcatcccc ccacttcctc tctctttact caggttcgac acacattttc
     1441 tcgttgccat gacgcatacg gaaagtccgc ctgcaagcgc aggctgccgc cacccacaca
     1501 tgagctatgc cctcatacgc aggctgcggg acgggttggt gggtgtgcta tgtgcacatc
     1561 ggctgccctc cttgtccggt tgtagattgc gggtacgact ttgaccaata ccctagacta
     1621 atgtggtact attactctgt acatagaccg aagcgagata cttctgtcga acgattacat
     1681 tttgcgacag gcagtcctaa tatgactgtc acagtttgtg tcacaagaaa gcgagcatcg
     1741 aacagtgccg gctgaatagc agcactatgc ccaccggtag ccggaggtcg gtatgagcaa
     1801 cttgtccgcg ttcgggtatt ggctgagaca gcagcgaaaa gaatacgaca tgagccagga
     1861 agacctggct gagcgtgtcg gctgcgcgta tgagacgata cgcaaaatcg aagtgggcag
     1921 ccgccgcccg tcaaagcaga tggcggagct gctgggtaag acgttcggca tcccggccgc
     1981 tgagctaccc gcttttatcg aatttgcccg gcaagaccca aacgcccccg gcaccggctc
     2041 ggacgcgccc ggcccggctg ccctcgccaa ccaacctata cgcgaaaaac aatcttacct
     2101 gggcacattg cccgcccagc gcaccagctt cgtggggcgc aaatctgaga tagaaggcat
     2161 cagggcgctg ctgttgagca gccacgtaag ggtccttacc ctcaccggcg caccgggcac
     2221 gggcaaaacg cggctggcgc tgaaagtagc ctccagcgtg caagccgagt tctccgacgg
     2281 agtgtacttc gtcgggcttg cttcgctctc ggacccggac aaggtgcctg gggtgattgc
     2341 tcgcgccctc aacatcaaag acgaaccgag ccgccccgct ttggcgctgg tgcaggagca
     2401 gttgcgcgag cgggtggtgt tgctgctgct ggacaacttc gagcacctga cctgggcagg
     2461 gtcgtacgtg ggcgagctag tggaggcctg ccccaacatc aaaattttga ccaccagccg
     2521 cactcccctt aacatctatg gcgagtacca gttctttata cgcccgatgg atctccccgg
     2581 aacccgagaa caactgggcg cggaggagtt gtaccagtac gaatcggcgg ccctgttcgt
     2641 ccaacgcgcc cgctcggtca atcctgagtt tcgcctgacc gcgggcaacg cggccgccgt
     2701 cgcggatatt tgccgccagc ttgatgggtt gcccctcgcc atagaattgg ccgccgccta
     2761 ctgcaaaatc ctgcctccgc acgccatccg gcagaagctt accagcaggc tcaacctgct
     2821 cacgcttggg gccagcgact tgcctcgccg ccagcagacg ttgagaggcg ctatcagttg
     2881 gagccacgac ctgctgtcgg cccagcaaga ggcgttgttc cgccgcgtgt cggtgtttgt
     2941 cggaggctgg acgctggaag cggtggaggg ggtctgcaac ctgcccgacg aggccgacgt
     3001 tggcaccata cagggcgtgg tcgcgctgtt ggaccgcagc ctcctcgagc gagaggacgg
     3061 cgcgggcggg cagccacgct tctcgatgct acagaccatc cgcgagtacg cccaggagca
     3121 gttgaacaag agcagcgaag tgcaactcat gcagcggcag cacgcccgct acttcctggc
     3181 gttcgccgag agcgccgaac cccacttgaa ggggcctgag cgcgacttgt ggctgcaacg
     3241 cctcgagctc gaacatgaca acttccgcac ggccctgacc tggtgcatgc atacccccga
     3301 ggccgccgat ttgggccttc gattggcggg cgcgttgcac tggttctggt acttccgtgg
     3361 ttatctcacc gaggggcggg actggctgga gggcacgctt gcgcgagccg ggggcctgag
     3421 gcacagcgcc gcgggagcca aggcactcga tgcggcgggc cacctggcgt tactccagga
     3481 cgattactcg gtgatgctgg accacctggc ggaaagcgtc tctacctggc gtagcgttgg
     3541 cgacaaacaa ggcctggcct acgcgctggc tgactacggg atagccacgg tgtaccggta
     3601 cagaggcaac tccgtggacg gcagcgaggc gctcaaggag gccatacaac tgttccgcga
     3661 ggtcggtgac aggtggggcc ttgcccttgc cctcaactgg cttggggatg ccacgggcct
     3721 gttgcgccac cccgaagatg cagtcgtggc ttataaaagc gagagccttg acctgtacag
     3781 gcagttggac gacaagtggg gcattgcttt ccagcttagt gagctgggcc gcctggcggt
     3841 atggcagggg aactacgagc tagcgcgggc aaggctggag gaatccctca ggacgggcgg
     3901 ttttggcgac aagtggtcgg tggcccacgt gcttcgcagc ctcggcgaca tagctttatt
     3961 tcagcacgac cgagcgcagg ctgtgctgct ctacgaagag agcctggccc tctaccgcga
     4021 gcttggcgac aagttccgcg gctctacctg cctgcgcaac ctggcgggca tctccctttc
     4081 ggagggcgag accgagcgcg cggcgcaact gtaccaggag agcctgcaac tcgccttcgc
     4141 gctacaaaac gtgccgaacg tggccttgtg cctcgccggt atggccgggg tggcgct
//
LOCUS       DASRWZ010000420         4807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_103840, whole genome shotgun sequence.
ACCESSION   DASRWZ010000420 DASRWZ010000000
VERSION     DASRWZ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103840"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFH60_06495"
     CDS             <1..221
                     /locus_tag="VFH60_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET6313466.1"
                     /translation="YSHEELARYAEPVPLDDLPPLDALVLQAYHDDYRALDWGKLSEH
                     GCRVVLDGRNALDSQAIERAGMRYIGIG"
     gene            237..1322
                     /locus_tag="VFH60_06500"
     CDS             237..1322
                     /locus_tag="VFH60_06500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313467.1"
                     /translation="MPEPSTRPLLKVLLLLPYEPAAFMQWDIDALGRHFELDVLVHNR
                     GKRRLFTSVLRRLLFDRPDVLLMWFVVPSYALPVTLMAKALGVKVGFFTGGFDVVSLP
                     SIGFGAMRFPLFRLFLKPTLALADLTLSMSESAAGQVRKYARPKRMVTLYQGIDTSFF
                     SPQPDVAREAIAVTVSPVIESSIVQKGLGAFVEAAQHAPDVKFVLVGRSPDGSIEKLR
                     AAASPNVEFVDRFIPSEDLRDLYARASCYVQASAHEGFGIAVAEAMACGAIPVVTRAF
                     SLPEVTGGLGEYVRFGDARAIAGAVRRTLSAPQERRDALRCHVTGNFTFERRERELVD
                     LLLPLAPGRRSIDSPGAGTGGQMLQGR"
     gene            1866..2441
                     /locus_tag="VFH60_06505"
     CDS             1866..2441
                     /locus_tag="VFH60_06505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313468.1"
                     /translation="MAIVSTPDLLEFAPGAHVLPGAVDLEQWTPKVARQERVSHAHPV
                     RILHAPTDRDIKGTKYLFAAVERLKGAGYPVEAILPEGLSQRQVHMLSAQADIAVDQL
                     MLGAFSMFTIEMMARGLPVVCRIREDLRQFYPPDLPVINAEPGSIYDVLESLITQPER
                     WAELGRRGIEYVRREHEMHRVAARSLSLYSM"
     gene            2637..3767
                     /locus_tag="VFH60_06510"
     CDS             2637..3767
                     /locus_tag="VFH60_06510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313469.1"
                     /translation="MRRRSARIFGERKLRVLHAPANIAGIAGLLARAQRDLGFDATSV
                     EYFQRPFNFGVDLSLGLQPTHGRVKKATTMGKFALRAFRRYDVFHLYFGNTLLPPPYP
                     DLPLLRALGKRIVFHFCGCEIRQREHNLRNYALSACTDCAWHRCKELRHPDLISADVA
                     FVSTPDLLAFVPGAHLLPGPVDLAKWAPRGGRREVISYAHPVRIVHGPTNRAVKGTKY
                     LLDAVQRLKSAGYPVEIDLLEGLPHEQIHALTEQADIAVDQLMIGAYGTFSVEMMARG
                     LPVVCRIREDLRRFYPPDLPVINAEPGSIYDVLESLITQPERWADLGRRGIEYVQREH
                     EMHRVAARALSLYNVDLSRGLAPAPGGAVTGFDRLESELRQN"
     gene            3810..>4807
                     /locus_tag="VFH60_06515"
     CDS             3810..>4807
                     /locus_tag="VFH60_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET6313470.1"
                     /translation="MLARLSSLSKDAVVYGLAGAAARVSGLVLLPVYTRVFEDTESYG
                     AWQIVSIFGALLVAVAALGLDGATSILFFDTDSPVERQRITTLWLLISLLVAVPLTVV
                     LVLLAGPISSLITGTDRYADLFRVGIVLLPFNMLQSVVAGVLRLQFRPRAFALLNVGL
                     TILVLSTSIYLVVGLRMEVMGALLGTLVGTALASIVGLWAVRSAVDWRLLTSEAWPVA
                     ARLLRLGAPLVPAAAALWVVSFSNTFFVARLLSTGDTGIFRVGAQIASVLGIAVYAFQ
                     FAWSPFSLSIAKAPDAHRVYTRVAMLYTAGSVGAAVLLGGAAPIVLLLAKGDYAPAS"
BASE COUNT          812 a         1511 c         1508 g          976 t
ORIGIN      
        1 tctacagtca cgaggagcta gcccggtatg ccgagcctgt gccgctagac gatctgcccc
       61 cgctcgatgc gctggtgcta caggcgtacc acgacgacta tcgtgccctg gactggggca
      121 agttgtcgga gcatggttgc cgcgtggtgc tcgacggacg taacgcgctc gattctcaag
      181 cgattgagcg ggcaggcatg cgctatatcg ggataggttg aggtccccag ccctccatgc
      241 ctgagccttc cacgcgcccg ctgctcaagg tgctcctact gctgccctac gagcctgccg
      301 ccttcatgca gtgggacatc gacgccctcg gccgccactt cgagcttgac gtgctggtcc
      361 acaaccgggg caagcggcgg ctattcacga gcgtgctgcg caggttgctc ttcgaccgcc
      421 ccgacgtctt gctgatgtgg ttcgttgtgc cgagctacgc cctgcccgtt accctcatgg
      481 cgaaggcgct cggcgtaaag gtggggtttt tcaccggcgg cttcgacgtg gtgagcctac
      541 cctcgattgg cttcggggcg atgcgcttcc ccctgttccg cctcttcctc aaacccaccc
      601 tggcgctggc ggaccttacc ctgtccatgt cggagagcgc cgccgggcag gtgcgcaagt
      661 acgcgcggcc caagcgcatg gtaacgctgt accagggcat agacacctcc tttttctcgc
      721 cacagcccga tgtagcgcgc gaggcgattg ccgtgaccgt gtcgccggtt atcgaaagct
      781 ctattgtgca gaaggggctt ggggccttcg tggaggcggc acagcacgca cccgatgtca
      841 agttcgtgct cgtaggccgc tctcccgacg gctcgataga aaaattgcgc gccgctgcct
      901 cccccaacgt ggagtttgtc gaccggttca tcccctccga ggacctgcgc gacctgtacg
      961 cccgcgcctc ttgctacgtt caagcgtcgg cccacgaagg gtttggcatc gcggtggccg
     1021 aggcgatggc gtgcggcgcg attcccgtag tcaccagggc cttttcgctg ccggaggtca
     1081 cgggcgggct tggagagtac gtccgcttcg gggacgcacg agcgatagcc ggggcggtgc
     1141 ggcgtaccct gtccgcgccg caagaacgcc gggacgcgct ccgctgccac gttacgggta
     1201 acttcacctt cgagcggcga gagcgggagc tggtcgattt gctgctccca ttagcgcccg
     1261 gcaggcggag catcgatagc cctggcgcgg gtaccggcgg gcaaatgctc cagggacgat
     1321 agcacccatg tctcacaatg tttgggcaat cccgccggca gataggcgtg gctgtgctat
     1381 aatttttagg tatgaaaccc ccacgtggca cagtcctgac ccagcgcaaa cccaggatcc
     1441 tccatgctcc ggctaatgtt gccggcagag cagggattct cgccagggcg caacgcgaac
     1501 tgggctttga cgcaacatcc gtcgaatact tccaacgtcc ctttggcttt ggtgtggacc
     1561 gctcactagg tctaaggtct aacgatagcc gtgccaagaa agcggcgagg atggggacat
     1621 ttgccctggg agcagttagc cgctacgatg tgtttcacct ctactttggt aataccttat
     1681 taccgcaccc ttaccccgat ttgccggtgc ttcgcgccat gggcaagcgc attgttttcc
     1741 atttttgtgg ctgtgatata cgccatcggg aacacaccct cagcacctat gcgctcagcg
     1801 cgtgtacgga ctgtgtttcg caggcgtgca agcgcctgaa gcacccggac ctcaccgcgt
     1861 gcgacgtggc cattgtatcg acgccggacc tgttggaatt cgctccgggg gcgcacgtac
     1921 tgcccggagc ggttgacctg gaacagtgga cgccgaaggt agcgcggcag gagcgagtat
     1981 cccacgcgca cccggtacgc atccttcacg caccgaccga ccgcgacata aagggcacca
     2041 agtacctgtt tgccgcggtc gagcggctaa agggggcggg ttaccctgtt gaagctatcc
     2101 tcccggaggg cctatcccag cggcaagtac atatgctctc cgcgcaggcc gatatagcag
     2161 tggaccaact gatgcttggc gcgttcagca tgtttaccat cgagatgatg gcaaggggct
     2221 tgccggtggt ctgccgcatc cgtgaggacc tgcgacagtt ctacccgccc gacctgcccg
     2281 taatcaacgc ggaaccgggc agcatttacg acgtactgga gtcgctgatt actcagcccg
     2341 agcgttgggc cgagctgggc cgccggggca tcgagtacgt caggcgcgag catgagatgc
     2401 accgtgtggc ggcccgttcc ctttctctat actccatgta aggaggggcg tttcgcttca
     2461 ggagatgagg ggaaggccag gagactatag attataggct tgctgtatgc ctggcgacgg
     2521 gaagacgcag cctcgacagc taccagggcg cggtcagcag ctagcgctca cttcgcaccg
     2581 ggccaggtgg catgttgcca ccttctacaa ggggctgtgt gctataattc gccggtatga
     2641 gacgcagaag tgcacgaatt tttggcgagc gcaaactcag ggtcctgcat gccccggcaa
     2701 atatcgcggg catcgcgggg ctgctggcga gggcgcaacg cgacctgggc tttgatgcta
     2761 cgtccgtcga atacttccag cgaccgttca actttggtgt tgacctgtcg ctgggtctac
     2821 agccaaccca tggccgggtc aaaaaagcga ccacgatggg gaagtttgcc ctccgggcct
     2881 tcaggcgcta cgatgtgttt cacctctact ttggtaatac tttgctgccg cctccctacc
     2941 ccgacctgcc actgctccgc gccctgggta agcgtatcgt cttccatttt tgcggctgtg
     3001 agatacgcca gcgggaacat aacctgcgta attatgcgct aagtgcctgc acggactgtg
     3061 cctggcatag gtgcaaggag ctgcgacacc cggacctcat ctcggcggac gtggcgttcg
     3121 tatccactcc cgacttgctg gcgttcgttc cgggggcgca tctattgccg gggccggtcg
     3181 atctcgcgaa gtgggcaccc aggggcgggc ggcgcgaagt gatatcgtac gcccaccccg
     3241 tccgcatcgt tcacggaccc accaatcgcg cggtgaaggg caccaaatac ctgcttgacg
     3301 cggtgcagcg cctgaagtct gccggttatc ccgtcgaaat agacctcctg gagggccttc
     3361 cacacgagca aatacatgcg ctcaccgaac aagcggacat cgcggtagac caactgatga
     3421 ttggcgcgta tggcaccttc tctgtagaga tgatggcaag aggcctgccg gtggtctgcc
     3481 gcatccgaga ggacctgcga cgcttctacc cacccgacct gcctgtaatc aacgcggaac
     3541 cgggcagcat ctacgacgtg ctggaatcgc tgattactca gcctgagcgt tgggcggacc
     3601 tcggccgtcg aggcatcgag tacgtacaac gcgagcatga gatgcaccgt gtggcggccc
     3661 gtgccctgtc cctgtacaac gttgacctca gcaggggcct tgcgcccgcg ccgggtggcg
     3721 ctgtgaccgg ttttgataga cttgagagcg agctaaggca gaattgacat acaatcctct
     3781 cctacccaat acttccgcga gccacgtacg tgctagcaag gctctctagt ctctccaaag
     3841 acgccgtcgt ctacggactg gccggggccg ccgcgcgcgt ctcagggctg gtgctgctac
     3901 ccgtgtacac ccgcgtcttc gaggacaccg agagctacgg cgcgtggcag atagtcagca
     3961 tcttcggcgc tctgctggtg gcggtggccg cactaggcct ggacggggcg acctccatcc
     4021 tcttcttcga caccgattct cccgtcgagc ggcagcgcat cacgacgctc tggctgctca
     4081 tctccttact ggtggcggtg ccgcttaccg tggtgcttgt cctgctggca ggccccatat
     4141 cgagcctgat tacgggcacc gaccggtacg cggacctctt ccgggtgggc atagtgttac
     4201 taccgttcaa catgctacaa tcggtggtgg caggggtctt gcgtttgcag tttcggcccc
     4261 gcgccttcgc cctgctcaac gtggggctga ccatcctggt gctctccaca agcatctacc
     4321 tggtggtggg cctgcgcatg gaggtgatgg gggcgctact gggcacgctg gtgggcacgg
     4381 cgctggcgag tatcgtgggg ctgtgggcgg tgcgctcggc ggtcgattgg cggctgctca
     4441 cctcggaggc ctggccggtg gcggcaaggc tgctgcggct gggtgccccg ctcgtgcccg
     4501 ccgccgccgc gctgtgggtg gtcagcttct ccaacacctt tttcgtggcc cgcctcctca
     4561 gcacggggga cacgggcata tttcgggtcg gcgcgcagat tgcttcggtg cttggcatag
     4621 cggtctacgc tttccagttc gcatggtcgc cgttcagcct ctccatcgcc aaggcgcccg
     4681 acgcccaccg cgtctacacc agggtggcaa tgctctacac agcgggttcg gtgggggcgg
     4741 cggtcttgct cggaggggcc gcccctatcg tgctgctgct ggctaagggc gactacgccc
     4801 cggcctc
//
LOCUS       DASRWZ010000421         4694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1200729, whole genome shotgun sequence.
ACCESSION   DASRWZ010000421 DASRWZ010000000
VERSION     DASRWZ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200729"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VFH60_06520"
     CDS             complement(<1..793)
                     /locus_tag="VFH60_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313471.1"
                     /translation="MGATLVLGACGGSGAGLSDVRASVGEIVPGSVGLGKPPGVVEVR
                     YRLGQEAEVRVELEGLASGSSLLHAERQAAGEHVLRFNGVISATNSVSSAEGYRVARE
                     VVPPGDYSIKVTANGSSESVGVRVGGTASQPPSIANMVLRPETISPNSDAIDDVTELT
                     FRTGQTATLSVDLVGPDGERIPVHAPIKKGPGEQNVVVSGDDLLGERLPDGSYMVTLR
                     VRDEQGNVVLAERPLTVEGSGEPAIAVLSVDISPEQLMLGDTVNVT"
     gene            complement(907..1881)
                     /locus_tag="VFH60_06525"
     CDS             complement(907..1881)
                     /locus_tag="VFH60_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HET6313472.1"
                     /translation="MQEAIARFPGVRVLVVGDLILDKYTIGKPVRISREAPVAVLEYQ
                     GDRVMPGGGTSPACTVASLGGRALLAGVVGQDESGRELVAELEQYGVDTGGIVIDVAR
                     PTITKKRIVAQVTPSLLQQVARIDHIDRRPVSGDVEDALIAAIEERLPRCDAVLLSNY
                     KGGTLTRRVVERTRQAATAQGKVLAVDSQGDLAMFHGFGIVKVNQAEAEETLRRPLDS
                     EASFEEGLPELLQMLEAQAVIVTRSAEGMSVMRQGGDYCHIPVTNSSEVFDVTGAGDT
                     VIAISTLAVASGTDLFDAARLANYGAGVVVRKWGNAVVTLDELRDAME"
     gene            complement(1967..2536)
                     /locus_tag="VFH60_06530"
     CDS             complement(1967..2536)
                     /locus_tag="VFH60_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HET6313473.1"
                     /translation="MYDTKDSTEDDAPLYCFNHPNEPTYLRCGKCERPICARCRVSTP
                     VGFRCFECANVQVLPTYAIDASVYLKAALYGLIAGGLSGVLMGLFPAFEFWGALLMGI
                     VVPEAVAFASNQKRGPGLQMVGLGCVVFGFIVSRFLLTQWGILIPLGRINIPSGSDIA
                     FFNDLPFYATQYTVLWLILAGFLTYKRLQ"
     gene            complement(2687..3436)
                     /locus_tag="VFH60_06535"
     CDS             complement(2687..3436)
                     /locus_tag="VFH60_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HET6313474.1"
                     /translation="MNILGVGVHNCDEREAVAAVEGFLRERPERLHQVCTVNPEFVME
                     ARRNRLFRDLLNRVDLATPDGAGIVAAARLLGKPVRGRATGVQLVEHLARLSARDGYS
                     LFLLGAQPGVADEAAEVLRRRYGEVRIAGTFAGSPRDEDWPEIRSRLAQASPDVLLVA
                     YGAPKQDLWIDRHSGDLPSSVKVAMGVGGVYDYLSGRAPLAPATMRRLGLEWLYRLVT
                     QPWRWRRILRVFVFGWLVCVDVMRRKVKLDE"
     gene            3929..>4694
                     /locus_tag="VFH60_06540"
     CDS             3929..>4694
                     /locus_tag="VFH60_06540"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313475.1"
                     /translation="MPHPFAIVALDQARRHNQQAGPDDPTLPPDAAVGLGLLLHYAVP
                     PELADTLRPGHMVVVPLRDRPAYGVVVELAESAPVEETRSILRLVDPQPILPAYMLEL
                     ARWISEYYRCTLWQAMSPMLPPGVARRAIVTVGLSADAFDGEDQDEHNPLIQALGRRQ
                     RQVMALLKDAPRSTLTLGKLKRKYEGAESGLHSAVQDLERKGLLTRRTELPSPRSRPQ
                     QERIIRLAVSHDEAAEAIRDSSHRAPLQAAALDWHTR"
BASE COUNT          887 a         1790 c         1208 g          809 t
ORIGIN      
        1 tggtcacatt aaccgtatcg cccagcatca actgctcagg ggatatgtcc acgctcaata
       61 cagctatcgc cggttccccg ctgccctcca ccgtcagagg ccgctccgcc agcaccacgt
      121 tcccctgctc gtcgcgcacc cgcaaagtca ccatgtagct gccgtccggc aacctctccc
      181 ccagcaggtc gtccccgctg actactacgt tctgctcgcc agggcctttc ttgataggcg
      241 catgcaccgg gatgcgctcc ccgtccggcc ctaccaggtc caccgacaaa gtggccgtct
      301 gcccggtgcg gaaagtcagt tcggtcacgt cgtcaatagc gtcgctgttg ggcgagatcg
      361 tttcgggccg caacaccata ttggcgatag atggaggctg cgaagcggtg ccccccaccc
      421 tgacacccac gctctcgctg ctgccgttcg ccgtcacctt gatagagtag tcccccggcg
      481 gcacaacctc ccgcgccacc ctgtaaccct ccgcgctgga cacggagttg gtggccgaga
      541 taaccccgtt gaagcgcagc acgtgctccc ccgccgcctg gcgctccgcg tgcaacaggc
      601 tgctgcccga cgccagcccc tccagctcca cccgcacctc ggcctcctgc cccagcctgt
      661 accgcacctc caccaccccc ggcggcttcc ccaaccccac cgaccctggc actatctccc
      721 ccaccgaagc ccgcacatcc gaaagccccg cccccgaccc cccacaagcc cccagcacta
      781 aagtagcacc caacaacacc aaaaacacca aaacgcgccg cccacccaga cgccgcgcaa
      841 atccaaaatc caaagcttgc cctcgagcga agcgaagggt ccaaaatcca aaatccaaac
      901 tcggtctcat tccattgcgt cgcgcaactc gtccaacgtc accaccgcgt tgccccactt
      961 acgcaccacc acgcccgcgc cataattcgc cagccgtgcc gcatcgaaca ggtcggtgcc
     1021 ggaagccacc gccagcgtcg aaatggctat caccgtgtcg cccgcgcccg tcacgtcgaa
     1081 cacctccgag ctgttggtca cggggatgtg gcagtagtcg cccccctgcc gcatgaccga
     1141 catcccttcc gcgctcctgg tgacaatcac cgcctgcgcc tcgagcattt ggagcagttc
     1201 gggcagcccc tcctcgaaac tcgcctcgct gtcgagcggg cggcgcagcg tctcctcggc
     1261 ctcagcctgg ttcaccttca cgatgccgaa gccgtggaac atcgccaggt cgccctgcga
     1321 gtcgaccgcc agcaccttcc cctgcgcggt cgccgcctgc cgggtgcgct ccaccacgcg
     1381 ccgggtgagc gtgccgccct tgtagttgct cagcagcacc gcgtcacagc ggggcagccg
     1441 ctcctctatc gccgcgataa gcgcgtcctc cacatccccg ctcaccggcc tgcggtctat
     1501 gtggtcgatg cgggccacct gttgcagcag cgacggcgtc acctgcgcta cgatgcgctt
     1561 cttggtaatc gtgggccgcg cgacgtcaat cacgatgccg ccggtatcga ccccgtactg
     1621 ctccagttcc gccaccaact cccggcccga ctcgtcctgc cccaccacgc ccgccagtag
     1681 ggcgcgaccc ccaagcgaag ccacggtgca cgcggggctg gtccctccac ccggcatcac
     1741 gcggtcgccc tggtactcca acacggccac cggcgcttcc cgcgaaatgc gcaccggctt
     1801 gcctatcgtg tacttgtcca gtatcaggtc gcccaccacc agcacgcgca cgcctgggaa
     1861 gcgagctata gcttcctgca agcggcgtct gccggcgctg tctgtatccg ggttatcggg
     1921 catagggtat aaggtagacg attgaaaggc cgtgcggttg cccgcgctat tggagccgct
     1981 tatacgtgag aaatccggcg agtatcagcc acagcacggt gtattgtgtg gcgtagaagg
     2041 gtaggtcgtt gaagaaggct atatccgagc ctgacgggat gtttattctg ccaagtggaa
     2101 taagaattcc ccattgcgtc agcaaaaagc ggcttactat gaagccgaac accacgcagc
     2161 ccaggcccac catctgcaag cccggccccc gcttctggtt ggaagcaaat gccacggcct
     2221 caggcaccac gatgcccatg agcagcgccc cccagaactc gaacgccggg aacagcccca
     2281 tgagcacgcc cgacagcccg cccgcgatca gcccatatag cgccgccttc aggtagacgc
     2341 tggcgtcgat cgcatacgtc ggcagcacct gcacgttggc gcactcgaag caccggaagc
     2401 ccaccggcgt gctcacccgg cacctggcgc aaatcggcct ctcacacttc ccacaacgca
     2461 ggtacgtcgg ctcgttgggg tggttgaagc agtacagcgg cgcgtcgtct tcagttgaat
     2521 ctttggtatc gtacaaacta gacattattt tgtggagcgg aacgagtaat gaggactgag
     2581 gactgagtgt accccatcct aatcatcatc cgctttgcgc gtcctttgcg ctctttgcgt
     2641 ctttgcggtt tcgtctctta actcagtcct cagtcctcat tactcgtcac tcgtccagct
     2701 tcacttttct cctcatcacg tctacgcaca caagccagcc gaatacgaac actcgcagta
     2761 ttcgccgcca gcgccagggt tgcgtcacaa gccggtagag ccactccagc cccagccgcc
     2821 gcatcgtggc tggggccagt ggcgctctgc ccgacaaata gtcgtacacc ccacccaccc
     2881 ccatcgccac tttgaccgac gagggcaagt cgccactgtg cctgtctatc cacaggtcct
     2941 gcttgggcgc gccataagcc accagcaaca catcggggct ggcctgcgcc agcctggaac
     3001 gaatctccgg ccagtcctca tctcgtggcg accccgcgaa cgtgcctgct atgcgcacct
     3061 cgccgtaccg ccgcctgagc acctccgccg cctcgtccgc aacccccggc tgcgctccca
     3121 gcaggaacag cgaatagccg tcccgcgccg acagcctggc gaggtgctcc acgagttgca
     3181 cccccgtggc cctgcccctc accggcttgc cgagcaaccg cgccgcggcc actatccccg
     3241 ccccgtccgg cgtcgccagg tctaccctgt tgagcaggtc gcgaaagagc ctgtttcgcc
     3301 gggcctccat cacgaactcc gggttgactg tgcacacctg gtgcagccgc tcaggacgct
     3361 cccgcaggaa cccctcgacc gccgcgaccg cctcccgctc gtcgcagttg tgcaccccca
     3421 caccgaggat gttcaccctc tccctactca atgccacacc ggaagtatat aacacggctg
     3481 tatggctggc aaacggccct ggaactgccc gtgtttagag atgagatggt gagaataaga
     3541 cagccagacg tcgcccgcga acaggaacgt gcatgtggtc cagttcaacc agaagaaggc
     3601 cgcgcaacct aacctcccac agcacatctc ttaagtggga ggttaggtta taggtactac
     3661 tctgaacttc tacctgtaac tcataatgcg attccaattt gttgtccgac cttgtctttc
     3721 ttgctgccgg taggacatag agcgcagtcg tgataagact gcgctctaga gcattgcaca
     3781 acatgtgcac acacccctgc cccaaagcat ataatgtcta taatcgacca caccattgat
     3841 attgccgcca tcggccaaaa acccttatct ctccacgcac caatcacaat ccgaaattcg
     3901 aaattcgaaa tcccaatttg taagacccat gccccaccct ttcgccatag tcgccctcga
     3961 ccaggcgcgc cgccacaacc agcaggccgg gccagacgac cccaccctgc cgcccgacgc
     4021 tgcagtgggc ctgggcctcc tgctgcacta cgccgtgccc ccggagcttg ccgacacgct
     4081 ccggcccggc cacatggtag tcgtgcccct gcgcgaccgc ccggcttacg gtgtggtggt
     4141 agagctggcc gagagcgcgc ccgtggagga aacccggtcc atactgcgcc tcgtagaccc
     4201 ccagcccatc ctccccgcgt acatgctgga gcttgcccgc tggatatccg agtactaccg
     4261 ctgcaccctc tggcaggcca tgtcgcccat gctgccccca ggcgtcgccc gtcgcgccat
     4321 cgtcacggtt ggcctctctg ccgacgcctt cgacggcgag gaccaggacg agcacaaccc
     4381 cctcatccag gccctcggca ggcggcagcg ccaggtcatg gccctcctca aggacgcccc
     4441 tcgcagcacc ctcactctcg gcaagctcaa gcgcaaatac gagggggccg agtcgggcct
     4501 gcattccgcc gtgcaagacc tcgaacgcaa agggctgctc acccgccgca ccgagctacc
     4561 ttccccgcgt tctcgccccc agcaggagcg catcatccgc ctcgccgtct cgcacgacga
     4621 agccgccgaa gccatccgcg atagctccca ccgcgccccc ctccaggccg ccgccctcga
     4681 ctggcacacc cgcc
//
LOCUS       DASRWZ010000422         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1269342, whole genome shotgun sequence.
ACCESSION   DASRWZ010000422 DASRWZ010000000
VERSION     DASRWZ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269342"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFH60_06545"
     CDS             complement(<1..758)
                     /locus_tag="VFH60_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313476.1"
                     /translation="MKNRTYTPNHRARAIRDAIFLLAVAIALGIGMALVSPASGAELR
                     QVGAGSIAGTVTVTGQSAAGLTVELRQRANSGEETVLGTATTDDTGSYSFPNQPSAPN
                     DAFYYIRLGSGKGTLATWYTFPIIYVQGNAFTVPSVDMSDVELVDPGQGATVSLPATL
                     TWKARRSGETYRIFVYASGNTGKVALDSGSLGTNTQFTIAEGALPDGTYEAVVQVRDA
                     VVGYGQSQSRFRFTIGKAAVENGQGAAPGVEPVAP"
     gene            complement(790..1740)
                     /locus_tag="VFH60_06550"
     CDS             complement(790..1740)
                     /locus_tag="VFH60_06550"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HET6313477.1"
                     /translation="MASRDTISRRGLWGRRDRGRQAGSIPQPEALAGENDRFRPSSLR
                     PVDGGRRRIALLPSQRREARNTAGQGASSLPLRIFDTALLVIEVVSAMVVSWLVWQYV
                     YTAYFDTAPRRVSPPRSEQVLPMTTPTRQPTPTRLAEVLPPLEGTSDGQEWTPGKVGG
                     ANAPPTATATPAPTVDPQILLPARLRIPAMFLDSPVHEVTVNMGEWEVSPMDVGHHEG
                     TANPGEMGNVVLAGHRDINSALFRDLDRLQPGDEVFVSNNLREFRYVVTESFVVGPDH
                     AEVMDPTDDKRVTLITCTPIGLATQRLVVTAILDETYSTR"
     gene            2324..>2681
                     /locus_tag="VFH60_06555"
     CDS             2324..>2681
                     /locus_tag="VFH60_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HET6313478.1"
                     /translation="MKKKTNRTTSFLLGALTLLVGLLVAAGIIMQLSGQAARDNIERA
                     NAFLLKERTVYSLMIDMETGERGYIITGDEAFLEPYNRAKAALPALWEELATIAADLD
                     RRSNTGPTRVAELVAEM"
BASE COUNT          448 a          780 c          895 g          558 t
ORIGIN      
        1 ggggcgacag gttctacgcc gggggccgcg ccctggccgt tctccactgc ggctttgcct
       61 atagtgaagc ggaaccggct ctggctctgg ccgtagccta ccaccgcatc gcgcacctgc
      121 acgaccgcct cgtatgtgcc gtcggggagc gcgccctctg cgatggtgaa ttgcgtgttc
      181 gtgccgaggc tgccgctatc aagggccacc ttgcccgtgt tgcccgacgc atacacgaag
      241 atgcggtacg tctcgcccga ccggcgggcc ttccaggtga gggtggcggg gaggctgacg
      301 gtagcgccct gcccagggtc aaccaactcc acgtccgaca tgtccaccga aggcacggtg
      361 aaggcgttgc cctggacgta gattatgggg aaggtgtacc aggtggcgag ggtgcctttg
      421 ccgctgccca ggcggatgta gtagaaggcg tcgttgggcg cgctcggctg gttggggaag
      481 ctgtagctgc cggtgtcgtc ggtggtagcc gtgccgagca ccgtttcttc gcctgagttg
      541 gcacgctggc gcaactcgac ggtgaggccc gccgcgctct gcccggtgac ggtaactgtg
      601 cccgcgatgc tgcccgcgcc cacctgccgc aactccgcgc cggaagcggg cgagaccagg
      661 gccatgccga tgcccagggc gatggcgaca gcgagcagga agattgcgtc cctgatggcc
      721 ctggccctgt ggttgggtgt atacgtgcgg ttcttcatgc ttcctcctat gcgtcctgcg
      781 agaccttctt taccttgtgc tgtatgtttc gtcgaggatg gccgtcacga ccaggcgttg
      841 ggtagccagt ccgatgggcg tacacgttat gagcgtgacg cgcttgtcgt cggtggggtc
      901 catgacctcg gcgtgatcgg gacccactac aaaactctcg gttactacgt agcgaaattc
      961 gcgcagattg ttgctcacaa agacctcgtc tccgggttgg agcctgtcca ggtcgcggaa
     1021 tagggccgag ttgatgtccc tgtgccccgc cagcacgacg ttgcccatct cgccggggtt
     1081 ggcggtgccc tcgtggtgcc ctacatccat cggtgatact tcccactctc ccatattgac
     1141 cgtgacctca tgcaccgggc tgtcgaggaa catcgccggt atccgcaggc gcgccggtag
     1201 caatatttgc ggatcaaccg tgggcgcggg ggtcgctgtc gcggtaggtg gcgcgttcgc
     1261 cccgccgact ttgcccggcg tccactcctg cccgtccgag gtgccttcca ggggaggcag
     1321 cacttcggct aagcgcgttg gagttggctg gcgtgtaggc gttgtcatgg gtaggacttg
     1381 ctccgacctg ggcggcgaca ccctgcgcgg ggcggtatcg aagtaggcgg tatagacgta
     1441 ctgccacacg agccaggaga ccaccatcgc gctcaccacc tctatcacaa gcagcgcggt
     1501 gtcgaatata cgcagaggca gcgagcttgc gccctggccc gccgtgtttc gcgcctcgcg
     1561 gcgttgtgac ggtaggaggg ctattcttct gcgcccaccg tctacggggc gcagactcga
     1621 cggcctgaag cggtcattct cacccgcgag cgcctccggc tgtgggatgc tgcccgcctg
     1681 cctgcctcta tctcggcggc cccacaggcc gcggcgcgat atggtatccc tagaagccat
     1741 agccgcgccc ggttggtaag ggtgtcgcac gcagccgccc ggcttgagta gaaggtaagt
     1801 ttggtgcggg taagtgatgc gtaatcatgg gtcgtatagc attcataggg tttggcggtg
     1861 ctgctcccgc aagcgcgggt gattccaggc gagtatatca agcatggggt atcgagcgtg
     1921 tagaaatcca caataggagc gtcagagttt tccacattgg gttggtgcgt ggcagaacca
     1981 gtaagtgtac gcagggtgtg ggcatgctcc ctgggcaccg cccggcgact gaagtcgcgg
     2041 gctacttcta cgaagtcgcc ctacggcgac tactgtctca gcatagtccg cacaggcgga
     2101 cttcgtttgc gtagcccgcg acttcagtcg ccgggcggtg cccagggagc ataaatagac
     2161 tttgtgaatg tactcctttc ccattgacaa ggggcaagga taacttttat gatggggtat
     2221 aagctttggc atgccgcgtc tgtgatggca tgagcttttg ccttgtgccc ggtgggactg
     2281 cggcttatat ccagggccat tagttgctgt gcttacattg attttgaaga agaaaactaa
     2341 tcgaactacc tcattcctgc tgggggcgct tacgctgctt gtcggcttgc tggtggcggc
     2401 gggtattatc atgcagttgt cggggcaggc ggcgcgcgac aacatcgagc gggcaaacgc
     2461 ctttctgctc aaagagcgca ccgtctacag cctgatgatt gatatggaga cgggggagcg
     2521 gggctatatc atcacgggag atgaggcttt cctggaaccg tataacaggg cgaaggctgc
     2581 gctgcctgcc ttgtgggagg agctcgccac tatagcggcg gacctggacc gacggagcaa
     2641 caccgggcca acccgcgtgg cggagttggt cgcggagatg c
//
LOCUS       DASRWZ010000423         4165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_584481, whole genome shotgun sequence.
ACCESSION   DASRWZ010000423 DASRWZ010000000
VERSION     DASRWZ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584481"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VFH60_06560"
     CDS             complement(<1..146)
                     /locus_tag="VFH60_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET6313479.1"
                     /translation="MRSYCVYILASASRTLYVGVTNNIVRRVYEHKCKKADSFTARYN
                     INRLV"
     gene            complement(324..1283)
                     /locus_tag="VFH60_06565"
     CDS             complement(324..1283)
                     /locus_tag="VFH60_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6313480.1"
                     /translation="MNICGVAADLLIASVLATTIRVATPLTLGAIGGLFSERSGVVNI
                     AIEGMMLIGAFSAYVVGALTGSLWWGLLGAILAAALMGLLHALLSVTFKVDQIISGTV
                     INILAFGITGFLYDQFFNRDAPSAGKLPTLSIPFLSDIPVWGRLFQHQPVVWSALLMV
                     AIAHFVFFFTPWGLRTRAVGEHPRAADTVGINVYFMRYANVIIGAGVAGLGGAYFTAE
                     VSGFTPGVTGGRGFIALAALIFGNWKPINALGATLLFGFTQALQINIQQCVSTSGTST
                     SDLPFFFLPQIVGLLPYVVTIVVLAGFVGRSSPPAAVGVPYDK"
     gene            complement(1280..2644)
                     /locus_tag="VFH60_06570"
     CDS             complement(1280..2644)
                     /locus_tag="VFH60_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6313481.1"
                     /translation="MSAISTNVDRTAGGSGGGDGPPPPAQRPSFIASVWAQSGRAFLV
                     PILAIFTALVLSGIVIWITTGDLQKVIGAKGAFAGLWQGAVGTPANIAETLMTATPYI
                     FAGLSVALAFKCGLFNIGAEGQLAAGAVTPAFVGYAIQGVPFPIHLILALAAGLLAGL
                     VWGAIPGVLKAFTGAHEVIITIMLNYIALGIVQYLLGGPMKDRTPGNVQARTPLIAPE
                     AELPQLFAGTDWQMHLGVPIAVLAAALVYWFLKRTTWGFEIKTVGANPHAARYAGMSV
                     ARSIILAMGLAGLLAGAAGAFEVAGVTRRHELGFGAGYGFDSIAVALLGRTNPFGVVA
                     AAVLFGGLKSGATEMQFNTQISGSIISVVQGLILLFVAADVIITWMYRLRVKPVGTSM
                     ALAPAALAASVGASAAEAPVDDAPEMEPIAESYPKTDPATGDDSPTTKERGSGRYEAG
                     GEEP"
     gene            complement(2795..4021)
                     /locus_tag="VFH60_06575"
     CDS             complement(2795..4021)
                     /locus_tag="VFH60_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6313482.1"
                     /translation="MRRRLFGRAGRALAVLMLAVPVIAACGGPAATPTSPAATNTTAV
                     DQATATTMVEQPTNTAAMEQPTNTVAMPEETPTEGTEATGTATGTTGDGTLKVGLVTD
                     VGRLNDKNFNQGSWEGVLRAKDELGVETNYIETSDPKDYDKNIQQFVDQDYDVIVTVG
                     FALAEATTKAAAANPDVMFIGVDQFQAEALPNLAGLIFDEDKAGYLAGVLAASISKSN
                     NIGAVLGTDDVPPVWKFGEGYRAGAKSVKPDITVQATYHSDVPITQTFIDPEWGKTTA
                     LSMVDRGADVVFGAGGLTGNGAIFAAKERNIPAIGVDVDQYETIGDEYKGSLVSSAMK
                     LLGPGVFNLIKAAQDGTFEGGNNVGEVGLAPYHDWADRVPAEVDAKLKEVDAALKDGS
                     LKTCVSPAKGAPSPCP"
BASE COUNT          771 a         1260 c         1378 g          756 t
ORIGIN      
        1 acaagacggt tgatgttgta gcgggctgta aagctgtcgg ccttcttgca cttatgctcg
       61 tatacccggc gcacaatgtt gttggtcacg cctacgtaga gggtgcgtga tgcacttgcc
      121 aggatgtaca cgcaatagga cctcatgtgt aactcctgga gagggtgcac tcttagcgat
      181 caagaaaggt gaaacatccc ggtgagggac gagattcttc gctacgctca gaatgacaaa
      241 gggggagagt gtcctgtaat agaacttatc attcagtcac gcactagcct cactgagcct
      301 cagtcctcgg tcctcagtcc tcatcactta tcatacggca cacccaccgc cgccgggggc
      361 gaggaccggc ctacgaagcc cgccagcacg acgatggtca ccacgtaggg gaggaggccg
      421 acgatttgcg gcaggaagaa gaacggcagg tcgctggtgc tggtgcccga ggtactcacg
      481 cactgctgga tgttgatttg caaggcctgc gtgaagccga acaggagcgt cgcgcccagc
      541 gcgtttatcg gcttccagtt gccgaagatg agcgcggcca gggcgataaa accgcgcccg
      601 cccgtgacac cgggagtgaa gccggacacc tcggcggtga agtacgcccc gcccaggccc
      661 gcgacgcccg ccccgataat gacgttggcg tagcgcatga agtagacgtt gatgcccacc
      721 gtgtcggcgg cgcgtgggtg ctcgcccacg gcgcgggtgc gcaggcccca gggcgtgaag
      781 aaaaagacga agtgggcgat tgccaccatc agcagcgcgc tccacacgac cggctggtgc
      841 tggaacagcc tgccccacac cgggatgtcg gagagaaacg ggatgctcag ggtgggcagc
      901 ttacccgcgc tgggggcgtc gcgattgaag aactggtcgt ataagaaacc cgtgatgccg
      961 aaggccagga tgttgatcac cgtgccgctg atgatctggt ctaccttgaa agtcacggac
     1021 aggagggcgt gcaacaggcc catcaaggcc gccgcgagga tggcacccag caggccccac
     1081 cagagggagc ctgtgagcgc ccccacaaca taggcgctga aggccccgat gagcatcatg
     1141 ccttcaattg cgatattcac cacaccggac cgctcggaga agaggccgcc tatcgccccc
     1201 agcgtgaggg gggtagccac gcgtatcgtg gtagccagca ccgaagcgat aagtagatcg
     1261 gcagcgacgc cacagatgtt catggctctt ctccgcctgc ttcatagcgg cccgaaccgc
     1321 gttctttagt tgtcgggctg tcgtctcctg tagcggggtc ggtcttgggg tagctctcgg
     1381 cgattggctc catttcgggg gcgtcatcca cgggggcttc ggcggccgag gcacccacgg
     1441 aggccgcgag ggcggctggc gcgagggcca tgctggtgcc cacgggctta actcgcaacc
     1501 tgtacatcca ggtgataatc acgtccgccg ctacgaacag cagaatgagg ccttgcacca
     1561 ccgagatgat agagcctgag atttgcgtgt tgaactgcat ctcggtggcc ccggacttga
     1621 ggccgccgaa cagtacggcc gccgccacca cgccgaacgg gttggtgcgc cccaggaggg
     1681 ccaccgcgat gctgtcgaag ccgtacccgg ccccgaagcc cagctcgtgc cgccgcgtta
     1741 cgccggccac ctcgaaagcc cccgccgcgc cagccagtag ccccgcgagg cccatcgcca
     1801 gtatgatgct gcgggctaca ctcataccgg cgtagcgggc ggcgtggggg ttggccccca
     1861 cggtcttgat ctcgaagccc caggtggtgc gcttgaggaa ccagtagacg agggccgcgg
     1921 ccagcacggc aatgggcacg ccgaggtgca tctgccagtc ggtgcccgcg aatagctggg
     1981 gtagctcggc ttcaggggcg atgagggggg tgcgcgcctg cacgttgccg ggggtgcggt
     2041 ccttcatcgg cccgcccagc aggtattgca cgatgcccag ggcgatatag ttgagcatga
     2101 tggtgatgat cacctcatgc gctcccgtga acgctttgag cacccctggt atcgcgcccc
     2161 agaccaggcc cgcgaggagg cccgccgcca acgccaggat gaggtgtatt gggaagggca
     2221 cgccctggat ggcatagccg acgaacgcgg gggtgacggc ccccgccgcc agttgccctt
     2281 cggccccgat gttgaacagg ccacacttga acgcgagcgc caccgagagg cccgcgaaga
     2341 tgtagggggt ggccgtcatg agggtttcgg cgatgttggc cggcgtgccc accgcgccct
     2401 gccacagtcc ggcaaaggcc cctttcgccc cgatcacctt ttgcaggtcg ccggtggtaa
     2461 tccagatgac gatgccgctg aggacgaggg ccgtgaagat cgccaggata ggcacgagga
     2521 aggcccggcc cgactgcgcc cacaccgagg cgataaagga gggccgttgt gcgggtgggg
     2581 gtgggccgtc acccccacca cttccgcctg ccgttcgatc tacattggtc gaaatagcac
     2641 tcaaggtgtt ctcccaccgg tacaacaagg ggcggtgtca cctgcattat acaagttaca
     2701 ccgccccggg ggaattagga tttgggaatt aggatttagg atttattacg tatccagcaa
     2761 atcctaaatc ctaattccca catcctaaat tggtctacgg gcaggggctg ggagcgccct
     2821 tggcggggga gacgcaggtc ttgagggagc cgtccttgag ggcggcgtcc acttccttga
     2881 gcttggcgtc tacctcggcg ggcacgcggt cggcccagtc gtggtagggg gcgaggccca
     2941 cttcacccac gttgttgccc ccctcgaagg tgccgtcctg ggcagccttt atcaggttga
     3001 acacgccagg cccgagcaat ttcatggcgc tcgacaccaa cgagcctttg tactcgtcgc
     3061 caatagtctc gtactggtca acgtccacgc cgatagcggg gatgttgcgc tccttggcgg
     3121 cgaagatagc gccgttgccg gtgaggccgc ccgcgccaaa caccacgtcg gcacctctgt
     3181 ctaccatcga gagggcggtg gtcttgcccc attcagggtc gatgaaggtc tgagtgatgg
     3241 gcacgtcgct gtggtaggtg gcctgcacgg taatgtcggg cttgaccgac ttggcgcctg
     3301 ccctgtagcc ctcaccaaac ttccataccg ggggcacgtc gtcggtaccg agcaccgcgc
     3361 cgatgttgtt gctcttgctg atggaggcgg cgagcacgcc ggccaggtag cctgccttgt
     3421 cctcatcgaa gataagcccg gccaggttgg ggagcgcctc tgcctggaac tggtccacgc
     3481 cgatgaacat gacgtcgggg ttggccgcgg cggccttcgt ggtggcctca gcgagcgcga
     3541 aaccgaccgt gacgatcacg tcgtagtcct ggtcaacgaa ctgctggatg ttcttgtcgt
     3601 agtccttggg gtcggaggtc tcgatgtagt tggtctcgac gcccagttcg tccttggcgc
     3661 gcaggacgcc ttcccacgag ccctggttga agttcttgtc gttgaggcgg ccaacgtcag
     3721 tgacaagtcc gaccttgagc gtgccatcgc ccgtagtgcc cgtggcggtg ccggtggctt
     3781 cggtaccctc ggtgggggtc tcttcgggca tggctaccgt gttggtaggc tgctccatag
     3841 ctgccgtgtt ggtaggctgc tcgaccatcg tcgtggcggt agcctggtcc accgcggtag
     3901 tgttagtggc agcgggggac gtgggggtcg ccgccggtcc gccgcaggct gcgattaccg
     3961 ggactgcgag catcaaaacg gccagcgctc gcccggcgcg gccaaacaat cgccttctca
     4021 taagactgct ttcctcctac taatgctaaa ggatctttgc cgtggcgact gtattatagc
     4081 acgagcgggc ggggcgtcaa ggggagtgat gaggactgag gactgagtga tgtgggaaag
     4141 cgcccaggcg cacagagcga ccaga
//
LOCUS       DASRWZ010000424         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_721205, whole genome shotgun sequence.
ACCESSION   DASRWZ010000424 DASRWZ010000000
VERSION     DASRWZ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721205"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1431
                     /locus_tag="VFH60_06580"
     CDS             205..1431
                     /locus_tag="VFH60_06580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313483.1"
                     /translation="MPATGFTRVKAAIFSHLSEQYDIHLIGIGYRGPATGVGGVTVHP
                     CNRKGGDMLGLYQAKDFIEQYRPELVFLLNDLWVLSRYVDALSPYQESTRIVCYCPLD
                     GKLVDESLAAPFSALAPGGRFVVYTEFARREMLRATEGMAFPPVKVIPHGVDVEAFYP
                     LAGSIERQLKQDGRLAAKRLLFRDRADLEDSFVVLNANRAQPRKRIDLTLQGFAMFAK
                     DKPAGVRLVLHHAVTFGEERAEVSRQAETLGIADRLTVGPAEGAGTSDEDLNLLYNAC
                     DVGINTSMGEGWGLVSFEHAATGAAQIVPRHSACAELWEGAAELVDPVNAYVPPFAML
                     EMQEVAPEGVAAALERLYADRAYRRDMSVKAYLNATQPAYRWERIAAQWGQLFNEMGA
                     GRELPSTSEYQLREQK"
     gene            1428..1919
                     /locus_tag="VFH60_06585"
     CDS             1428..1919
                     /locus_tag="VFH60_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6313484.1"
                     /translation="MNITLRPIVQGDEELLYRVYASTREAELALTDWTDEQKAAFVQM
                     QFVAQHNHYQEYYPNAAFLVILADEQPVGRLYVDRWPDQTRVVDITVLPEYRNLGIGT
                     VLLKALMAEAAEAGKPLTIHVEQYNPALRLYERLGFTRIADRGIYYLMEWRPEGVQSV
                     EAK"
     gene            complement(1912..>2200)
                     /locus_tag="VFH60_06590"
     CDS             complement(1912..>2200)
                     /locus_tag="VFH60_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313485.1"
                     /translation="TFSPHVGGTFRVPYDSSSAVELTLALAEEFGTESAREWREASGR
                     APFTLTFVGPTGIFLPQGIYRVEHDELGTFEIFLVPLGPGEGGLLYEAIFT"
BASE COUNT          441 a          676 c          706 g          377 t
ORIGIN      
        1 cagggacggg agcggctttg aggagttctc ccactttggc gtgataccta tcatgacctt
       61 ctcactcatg ccagtaaccg tggctggtaa gaccaggtac tttcctatca gaacctggca
      121 ctaacactgg cagccgggac cgcaagcacc caaaaaccaa ccgccggacg gggcaagccc
      181 tgcctcctgg cggttggttt ttgggtgccc gccaccggct tcacgcgggt gaaggccgct
      241 atcttctccc acctgtccga gcagtacgac atccacctca tcggcatagg ctaccgtggt
      301 cccgctacgg gcgtcggtgg cgtcaccgtc cacccctgca accgcaaggg cggcgacatg
      361 ctcgggctgt accaggcgaa agactttatc gaacagtacc ggccggagct ggtgttcctc
      421 ctcaacgacc tgtgggtgct gagcaggtac gtggacgcgt tgtcgcccta ccaggagagc
      481 accaggatcg tgtgttactg cccgctcgac ggcaagctgg ttgacgagag cctggcggcc
      541 cccttctccg ccctcgcgcc gggaggccgc tttgtcgttt acaccgagtt cgcccggcgg
      601 gagatgctac gggcgacgga gggcatggct ttcccccctg tgaaggtgat accccacggc
      661 gtagatgtcg aggctttcta cccccttgca ggctccatag agaggcaatt gaagcaagac
      721 gggaggcttg ccgccaaacg cttgcttttt cgcgacagag cagacctgga ggactcgttc
      781 gtcgtgctca acgccaaccg ggcccagcct cgcaagcgca tcgacctgac cctccagggc
      841 tttgccatgt tcgcgaagga caagcccgcc ggcgtcaggc tcgtcttgca ccacgccgtc
      901 acgtttggag aggagcgggc ggaggtctcg cgccaggccg aaacgctcgg aatagccgac
      961 cggctgacgg tcggcccggc ggagggcgcg ggcacgagcg acgaagacct gaacttgcta
     1021 tataacgctt gcgatgtagg tatcaacacg tctatgggcg aggggtgggg cctggtgagt
     1081 ttcgagcacg cggccaccgg ggcggcccaa atagtgccca ggcacagcgc gtgcgccgag
     1141 ctgtgggagg gggccgccga gcttgtggac cccgtaaatg cttacgtgcc cccgtttgcg
     1201 atgttggaga tgcaggaggt cgcgccggag ggggtggcgg ccgcgctcga acgactgtat
     1261 gctgataggg cctacaggcg ggatatgtcg gtaaaggctt atctcaacgc cacgcagccc
     1321 gcataccgtt gggagcgcat cgccgcgcag tggggccaac tattcaacga gatgggggcg
     1381 ggacgcgagc tcccatcgac atcagagtac cagcttagag agcaaaaatg aacattacct
     1441 tacgaccgat agtgcagggc gacgaagagt tgctttaccg ggtgtatgcc agcacgcgag
     1501 aggcggaact ggccctgacc gactggaccg acgagcagaa agcagccttc gtgcagatgc
     1561 agttcgtcgc gcagcacaac cactaccagg aatactaccc gaacgcggcc ttcctggtca
     1621 tcctggcgga cgagcagccg gttggccgcc tctacgtgga ccgctggccc gaccagacca
     1681 gggtggtgga cattacggtg ctccccgaat accgcaacct ggggatcggg accgtgctgc
     1741 tgaaggctct catggccgag gccgcggagg cgggcaagcc gctcaccatc cacgtggagc
     1801 aatacaaccc cgcgctacgc ctctacgaga ggctgggctt cacccggatt gccgaccgag
     1861 gtatctacta cctgatggaa tggcggcctg aaggtgtgca gagcgtggaa gctaagtaaa
     1921 gatagcctcg tagagcagac cgccttcacc ggggccgagg ggcacgagga agatctcgaa
     1981 cgtgcccagc tcgtcgtgct ctacacggta aatgccttgc gggagaaaaa tccctgtggg
     2041 gccgacaaac gtgagggtga acggcgcgcg gccggaggcc tccctccact ctctggccga
     2101 ctcggtgcca aattcctccg ccaaggccag tgttagctcc accgcggatg aagagtcgta
     2161 cgggacccga aacgtgcctc ccacatgagg agaaaatgtc
//
LOCUS       DASRWZ010000425         6319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_379418, whole genome shotgun sequence.
ACCESSION   DASRWZ010000425 DASRWZ010000000
VERSION     DASRWZ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379418"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFH60_06595"
     CDS             <1..217
                     /locus_tag="VFH60_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313486.1"
                     /translation="YSVIDLEPLEMANPPTEPSSPVPARDIPLGVALGLALGVLLAIA
                     LDYLVGSRGVQSELRALHPYYNQEVSR"
     gene            283..2469
                     /locus_tag="VFH60_06600"
     CDS             283..2469
                     /locus_tag="VFH60_06600"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET6313487.1"
                     /translation="MYAPTLRPPPAILDPGVTRLFALVTVLAGLLIGFAAGLGMPQNL
                     LVALAVSPFLLMFIYARPHWAVTVYVVLVYADLLSIMVKYQGFPPLARFAGAIMLSAV
                     LGYRLIYRREGLKKDEVTPWLIGYGLVIALGLLYARDPNLVMPNLVEFARNFLTYLII
                     INSITSMGRLRATLWGLLGIATLLSMLTLYQTVTGNSDSDFGGLAQFRVSEISGGQDA
                     PRPGGTIGDANYYGQLLLIVVPVGLYLLFESRTFLSKLAAMGATTLVIGAIVFTYSRG
                     DAVALGATLFAAMLFKRPRLHVLLLMLLGGVLALAALPSNYVERMSIVFDALTGNQRA
                     ILTEYSLRGRAGAVSAAVDMFADHMLLGVGRENYPLYQLDYLEGSALALHNRAIPPHD
                     LYLEIATEHGAVGLVVFAGLILACVKALREARRRFMALLDREGAELAGWLGIGLFGYL
                     VSGLFLHGAFLYMLWLQVALIVALRQISRNAEVVVPRPAPIPPDEQEPVLPFADPAAP
                     RTYLMPVAPRSPGILSALLPRRREKWSVEPVGQAIAGKPAAAPVPPTPVELLRRFYEA
                     NGGEAVFGKPLSPPFQEQGADGHPALVQYYEYGRLEYRQSGAAQEEVVLGKLGFEVAV
                     SGSPVDPLPPALQGEQVRLYGDTDAGPGVPPAFYEAWQTGGPDLFGLPISPVLLDTTP
                     AGLPIYVQYFERARLEYHPENAGTGYEVQLTPLGLEVYKARYGVTS"
     gene            2469..3440
                     /locus_tag="VFH60_06605"
     CDS             2469..3440
                     /locus_tag="VFH60_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6313488.1"
                     /translation="MPASSFTVLSYHRIAEPGCPDLADSLIDASPAAFEEQMRHIASR
                     YNVVSSWDLVRALTEGYTLPSRAVAITFDDGYHCFLDTAMPVLRKLGLPVTLFVATHL
                     AGEPGALFWWDELYRAIRGTELQELEVAGLGMLPLSNKQERADAFSRLVDHIERLPQE
                     EDTALLRSIVARSGVEPNRESYLLDWDQLRALEAEGVAIGPHSRHHPILAQATPHRVT
                     SETRGSWRDLRAQLQSPLPIFCYPNGREHAVNHTATEAAKETGLVAAFTTSPGLNVVG
                     KTDPYRLYRVGATAGESLRHFAIKLSPAGRAYRGLKSLVRRERDNGR"
     gene            3495..4160
                     /locus_tag="VFH60_06610"
     CDS             3495..4160
                     /locus_tag="VFH60_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET6313489.1"
                     /translation="MMQHVPLAPYQSNVGPKRRRRFRPLRVMLLLAALLAVWLGGDLA
                     YVLIGAETDYAAPADVIIVLGCSPYGPRGGPSVCLSSRGQHAANLYHKGLAEHIIATG
                     GHTENGPTEASVMLRVLVSAGVPESAVTLEEQAHNTIQNIGYSQEIMRRNNWDTAILV
                     TEPYHIKRAALVAQDAGLRVYPSPAVDSPLWRNPDQRRFRVLRDTLSLVLYQLKVASG
                     NRE"
     gene            4281..4718
                     /locus_tag="VFH60_06615"
     CDS             4281..4718
                     /locus_tag="VFH60_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313490.1"
                     /translation="MIAWGVLGGVALGFVYVLLLFFFTGSNATLVIYVAMVGSLFGGF
                     VGLVLGLLDGLVMTAIALLMLPRRPSWRTFRAIVYLVSALLTASGALYAFSEIAASFR
                     LANSGGLLFAVLPTLISVVLALLAASRVAAWVERNAELGARPA"
     gene            complement(4748..>6319)
                     /locus_tag="VFH60_06620"
     CDS             complement(4748..>6319)
                     /locus_tag="VFH60_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HET6313491.1"
                     /translation="IARSHFLMNKDTTLDSDTPIEAPGPGGGLGRQAAQGAAWNYLAF
                     VVSKGLVFASTLILAQILSPAEFGLVGMALLVITVLDILRDFGIGSAVIYYGREGPAA
                     ANMAFALSSGIGVFLFVANWLLAPFTAQFFYTDSPQDIPVVTSLIQVLGFSLLFASLG
                     STHDALLQKEINYRRRMVPEVGRTLLKGVLSVALAFAGLGVWSLVWGQVLGEACATVL
                     LWVVAKWRPARLFRRDLLRSMLGYSFQTMLAGGLGTMLADVDYFIIGALLGPTALGIY
                     TLAFRLPDLLIRNMAQAVATVAFAVAAKLQSDLAAMRDAYLRMQRYMLIILAPLGFGL
                     FAVTPGLINTLFKPDWAPVIPVMQILCVYMVLGAINYWPGVIYKAVGRPDVLNYISAV
                     KLGMLVPVLWWSATNYGIEGVAWGQLVVRLVGIVIDMVIVARFVQISVWDNLRVIWPP
                     LLAACLMSAAVRLLYFAVDPTERGGIPMLGLSVVVGSAVYVAAIWLLDRTALSGMLDL
                     ARSMLKRNRLAPREA"
BASE COUNT         1133 a         2168 c         1880 g         1138 t
ORIGIN      
        1 ctacagcgtc atagacctcg aaccgctgga gatggccaac ccgcccaccg aacccagctc
       61 acccgtcccc gcccgcgaca taccgctcgg agtagccctc ggcctcgccc tcggcgtgct
      121 cctggccatc gccctcgact acctcgtagg ctccagaggc gtccagagcg aactccgcgc
      181 cctccacccc tactacaacc aggaagtaag cagatagaac gattttagat tttggattgt
      241 ggattagaag cgcaatccaa aatctaaaat ctaaaatccg tcatgtacgc tcctactctc
      301 aggccaccgc ccgccatact cgacccaggc gtcaccaggc tgtttgcgct ggtgacggtg
      361 ctggccggcc tcttgatcgg gttcgcggcc gggttgggca tgccccagaa cctgcttgtt
      421 gcgctggcgg tcagcccctt cctgctcatg ttcatctacg cccgaccgca ctgggccgtg
      481 accgtgtacg tggtgctcgt ctacgccgac ctgctcagca tcatggtcaa gtaccagggc
      541 ttcccgccgc tggcacgctt cgccggggct atcatgttga gcgcggtgct aggctacagg
      601 ctcatctacc ggcgcgaggg gctgaagaag gacgaggtca ccccctggct catcgggtac
      661 ggcctggtga ttgcgttggg cttgttgtac gctagagacc ccaacctcgt catgccgaac
      721 ctggtggagt tcgcccgcaa cttcctcacc tacctcatca tcatcaactc gataaccagc
      781 atgggcaggc ttagagcgac gctctggggc ctcctaggca tcgccacgct actctccatg
      841 ctcactctct accagacggt gacgggcaac tcggacagcg actttggcgg cctggcacag
      901 ttccgcgtca gcgagatctc cggcggccag gacgcgcccc ggcccggcgg cacgattggc
      961 gatgccaact attacggcca gctattgcta atcgtggtgc cggtcggcct ctatctgctg
     1021 ttcgaatcgc gcacgttcct gtcgaagctg gccgcgatgg gagctaccac actcgtaatc
     1081 ggggcgatag tcttcaccta ctcccgcggc gacgccgtgg ccctgggggc taccctgttc
     1141 gcggccatgc tcttcaagcg gccccgcctc cacgtactcc tgctcatgtt gctgggcggt
     1201 gtgctggccc tggcagcgct ccccagcaac tacgtcgagc gcatgagcat agtcttcgac
     1261 gcgctcacgg gtaaccagcg ggccatactc accgagtact ccctgcgggg ccgggccgga
     1321 gccgtcagcg ccgccgtcga tatgttcgcc gaccacatgc tgctgggcgt gggccgcgaa
     1381 aactaccccc tgtaccagct tgattacctg gaaggcagcg ccctcgccct gcacaaccgc
     1441 gccatacccc cgcacgatct ctatcttgag atagccaccg agcacggggc ggtggggctg
     1501 gtggtctttg ccgggctgat tctcgcttgc gtcaaggctt tgcgcgaggc ccggcggcgt
     1561 ttcatggcgc tactcgaccg ggagggcgcg gagctggcgg gctggctcgg tatcggtctg
     1621 ttcggctacc tggtgagcgg cctgttcctg cacggggctt tcctctacat gctatggtta
     1681 caggtagccc tcatagtggc cctgcggcaa atctcgcgca acgccgaagt ggtggtgccg
     1741 cgcccggcac caattccccc cgacgagcaa gaacccgtgc tgcccttcgc cgaccccgcc
     1801 gcgccccgga cctacctcat gcccgttgcg ccccgttccc ccggcatctt gtccgcgctc
     1861 ctaccgcgcc gccgggagaa atggtcggta gagcctgtag ggcaagccat agccggcaaa
     1921 cctgctgccg cgcctgtgcc gcccacgccc gtagaactgt tgcgtcgctt ctatgaggcc
     1981 aatggcggcg aggcagtgtt cggcaagccc ctcagccccc ctttccagga gcagggcgcg
     2041 gatggccacc cggcgctcgt gcagtattac gagtacggcc ggttagagta ccgtcagagc
     2101 ggggcggcac aggaggaagt tgtgctgggc aaactgggat ttgaagtagc tgttagcggt
     2161 agccccgtag accctctgcc ccccgcgttg caaggcgagc aagtgcgcct ctacggagat
     2221 acagacgccg gaccgggcgt gccccccgcc ttctacgaag cctggcaaac gggaggccct
     2281 gacctgttcg gcctgcccat cagccccgta ttgctggaca ccacgcccgc gggcctgccc
     2341 atctacgtac agtacttcga acgcgcacgc ctggagtacc accctgagaa cgcgggcacc
     2401 ggctacgaag tgcaactcac acccctcgga ctagaggtat acaaggcccg ctatggagtg
     2461 acctcataat gcctgcttca agcttcaccg tgctctccta ccaccgcata gccgagcccg
     2521 gttgcccaga cctcgccgac tcgctgatag acgcttcccc cgccgctttc gaggagcaga
     2581 tgcgacatat cgcctcgcgc tacaacgtcg tgtcttcctg ggacctggtg cgcgccctca
     2641 ccgaaggcta taccctccct tcccgcgccg tcgccatcac ttttgacgac ggctaccact
     2701 gcttcctcga taccgcgatg cccgtattgc gcaagctagg tctgcccgta actctgtttg
     2761 tagccacgca cctggcaggg gagcctggcg cgctattctg gtgggacgaa ctgtaccgcg
     2821 ctattagagg caccgaactg caagaacttg aggtcgccgg cctcggcatg ctgccattga
     2881 gcaacaagca ggagcgtgct gacgctttct cccggctcgt ggaccacatc gagcgattgc
     2941 cccaggagga ggacaccgcc ctcctgcgct ctatcgtggc ccggtccggc gtcgagccga
     3001 accgcgagtc ttacctgctc gactgggacc agctacgcgc cctggaagct gaaggcgtgg
     3061 cgataggccc tcactcccgc catcacccca ttctggcgca ggcgacgccc caccgcgtca
     3121 cctccgagac gcgcggctcc tggcgcgacc ttcgcgcgca gctacaaagc cccttgccta
     3181 tcttctgcta ccccaacggg cgagagcatg ccgtgaacca caccgcgacg gaagccgcca
     3241 aggaaactgg cctcgtcgcc gcgttcacga cctcgcccgg cctcaacgtc gtgggcaaga
     3301 ccgacccata ccgtctctac cgcgtcggtg ccacggcggg ggagtcgttg cgccatttcg
     3361 ctatcaagct cagccctgcc ggtcgcgcct atcgtgggct aaagtcgctc gtgcggcggg
     3421 agcgggacaa tggacgatag aggacgggcg attggcggca gcaatagcgt cactcgtcca
     3481 cgtcccaacg cccgatgatg caacacgtac ctctggcccc gtaccagtcc aacgtggggc
     3541 ctaagcgccg cagacggttc aggccgttgc gggtgatgct gctgttggcg gccctgctcg
     3601 cggtgtggct tggaggagac ctggcctacg tgctaatcgg ggccgagacc gattacgcag
     3661 cccctgccga cgtgataatc gtgcttgggt gcagccccta tggtccacgc ggcgggccga
     3721 gcgtctgctt aagctcgcgt ggccagcacg ccgccaacct ctaccacaag ggcctggcgg
     3781 agcatataat cgcgactgga ggccacaccg agaacggccc caccgaggcc tcggtaatgc
     3841 tgcgagtgct cgtgtcggcc ggcgtgcctg aatcggcggt caccctggaa gagcaggccc
     3901 acaacacgat acagaacatc ggctacagcc aggagataat gcggcgcaac aactgggaca
     3961 ccgctatcct cgtgaccgaa ccgtaccaca tcaagcgcgc ggccctcgtt gcccaggacg
     4021 ccggtctgcg cgtctacccc tcgcccgccg tggacagccc tctctggcgc aaccccgatc
     4081 agcgccgctt ccgcgtgctg cgcgacacac tttccctcgt gctctaccag ctaaaagtag
     4141 ccagtggcaa ccgcgagtaa tagcctttcc aaactcatgt ggtagagtag aatagacgat
     4201 ggtcggatgg catccaggta gagcaacgcg ggggtggtca catgaatacg tggagtctag
     4261 tccgggggat taccgggcgc atgatagcct ggggtgtcct ggggggcgta gctctcggct
     4321 tcgtttacgt gctgttgctg ttcttcttca cggggagcaa tgccaccctt gtcatttacg
     4381 tcgctatggt cgggtctctt ttcggaggct tcgtgggcct tgtcttgggg ctgctcgacg
     4441 ggcttgtgat gactgccatc gcgctcttga tgctgcctcg caggccaagc tggaggacgt
     4501 tccgggcaat cgtgtacctc gtgagcgcgt tacttacagc ctcgggtgct ctttacgcgt
     4561 tttcagagat tgcggcttct ttcaggctgg cgaattccgg cgggttgctc tttgccgtcc
     4621 taccaacgct catctccgtg gtcctcgcat tgctagctgc aagccgtgtg gcagcctggg
     4681 ttgaacgtaa tgcggaacta ggagccaggc cggcgtaggt gacgtcccgg acgtctctca
     4741 gcccccacta agcctcgcgg ggtgccaggc ggttgcgctt tagcatcgag cgtgcaaggt
     4801 cgagcatgcc gctaagggct gtgcggtcga gcagccagat ggccgccacg tacaccgccg
     4861 agcctaccac caccgaaagg cccagcatag gtataccccc ccgctccgtc gggtcaaccg
     4921 cgaaatacag caggcgcacg gcggcggaca tcaagcaggc ggcgagcagg ggcggccaga
     4981 tcacccgaag gttgtcccac acgctgatct ggacgaagcg ggccacgatc accatgtcta
     5041 tcacgatgcc caccaggcgc accaccaact gcccccaggc caccccctcg atgccgtaat
     5101 tcgtcgcgct ccaccacagc accggcacaa gcatccccag cttcaccgcg ctgatgtagt
     5161 tcagcacgtc gggccgcccc accgccttgt aaatcacccc cggccagtag ttgatggccc
     5221 ccagcaccat gtagacgcac agtatctgca tcaccgggat gacgggtgcc cagtcgggct
     5281 tgaacagcgt gtttataagg ccgggggtga ccgcgaacag gccgaagccg agcggagcca
     5341 gtattatcag catgtaccgc tgcatccgca ggtacgcgtc gcgcatcgcc gccaggtcgg
     5401 actgcaactt cgccgccacc gcgaacgcca ccgtggctac ggcctgcgcc atgttcctta
     5461 tcagcaagtc gggcagccgg aaggcgagcg tgtagatgcc cagggccgtg gggccaagca
     5521 gcgcgccgat gatgaaatag tccacgtcgg ccagcattgt gcccagcccg cccgccagca
     5581 tcgtctggaa gctgtagccg agcatcgagc gcaggaggtc gcggcggaag agccgggcgg
     5641 gccgccactt cgccaccacc cacagcagca ccgtcgcgca cgcctccccc agcacctgcc
     5701 cccacacgag gctccacacc cccagccccg cgaaggccag cgccaccgaa agcaccccct
     5761 tgagcagcgt cctgccaacc tccggcacca tgcgccgccg gtagttgatc tccttctgca
     5821 aaagcgcgtc gtgcgtgctg cccagtgagg cgaacagcag gctgaagccc agcacctgta
     5881 tcagcgaagt gactaccggg atgtcctgcg ggctgtcggt gtagaagaac tgcgccgtaa
     5941 atggggccag cagccagttg gcgacgaaga ggaacacccc tatgccgcta ctgagcgcaa
     6001 acgccatgtt ggccgccgcc ggtccctctc gcccgtagta gattaccgcc gagcctatcc
     6061 cgaagtcgcg caggatgtcg agcacggtaa tcacaagcag ggccataccc accaggccaa
     6121 actcggctgg cgagagtatc tgcgccagga tgagggtgga cgcgaacacc aggcccttgc
     6181 tcaccacgaa agccaggtag ttccaggcgg ccccttgcgc cgcctgcctg ccgaggccac
     6241 cgcctgggcc gggtgcttct atgggagtgt cgctatccag ggtggtatcc ttgttcatga
     6301 ggaagtgtga gcgcgcgat
//
LOCUS       DASRWZ010000426         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_859123, whole genome shotgun sequence.
ACCESSION   DASRWZ010000426 DASRWZ010000000
VERSION     DASRWZ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859123"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFH60_06625"
     CDS             <1..442
                     /locus_tag="VFH60_06625"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6313492.1"
                     /translation="TLGIITLMFVLLFKFLPDVQVAWKDVVVGAFITAVLFTAGQFAL
                     GFYVTSGSVGTAFGAAGSIVIILVWVYYSSLIFFIGAELTQVYARRFGSRVVPDSNAE
                     PLTDEARAQEGIPKKDGDDKDRTGGGDGKSRPKHRSLKASPWFR"
     gene            583..1839
                     /locus_tag="VFH60_06630"
     CDS             583..1839
                     /locus_tag="VFH60_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6313493.1"
                     /translation="MKGNSLLGLVKETFQDWTQDKASRLGAALAYYAIFSIGPMLVVV
                     IAIAGKVFGKDAAEGKISDTLSQVLSSDAAKFVEALVTSASKPKEGTIATVVGTGTLL
                     LSAMGVFGQLKDALNTIWEVEPVPGGGIMAALTKNFLSFGMLLGVGFLLLVSLVVNAL
                     LSTLGPFLRDALPGGALLWNAVNYVITLAIISLMFALIFKFVPDIRVGWKEVLLGGVV
                     TATLFLLGQIALGIYLSLGNVGSAFGAAASLVIVLVWIYYSAQILFLGAEFTQVYANR
                     YGSGMAPGRHARLLTEEARAQQGTKSKQPRKQAAREQEAEPGSPTHKTSPWFRYVSHV
                     ETELPRRQARAKDHGKRMKGLGCRGTMHQAPTAICLCRTRGTVFPLVILSVAKDQPPV
                     LDPLYCCTAVPHATSSQKVEDSGLPT"
     gene            2052..2573
                     /locus_tag="VFH60_06635"
     CDS             2052..2573
                     /locus_tag="VFH60_06635"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015153210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HET6313494.1"
                     /translation="MSDKISIVQGDITKLAVDAIVNAANNSLLGGGGVDGAIHRAAGP
                     KLLAETRTLGGCPTGEARITGGYNLPAKWIIHTVGPVWRGGNAEEDALLASAYRNSLK
                     LAGEHGARTIAFPSISTGAYGFPVERAARIALREMSDFLAAGPRSVEQVILVAYGERT
                     YKAYTEALEEIDA"
     gene            2575..>2908
                     /locus_tag="VFH60_06640"
     CDS             2575..>2908
                     /locus_tag="VFH60_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET6313495.1"
                     /translation="MQAFIFDMDGVIVDSELHWKKPEGYFLRSLVPNWTDADQTRIIG
                     LSLSNVYRMLTTEYGVTHSKEEFLEVYMAMARQIYGELSSLMPGFLVLLRELHRRGTP
                     VAVASSSTR"
BASE COUNT          557 a          902 c          893 g          556 t
ORIGIN      
        1 cacactgggc ataatcaccc tgatgtttgt cttgctcttc aagttcctgc ccgacgtgca
       61 ggtagcctgg aaagacgtgg tggtcggggc cttcattacc gcggtcctgt tcacggcagg
      121 ccagttcgca ctcggttttt acgttaccag cggcagcgtc ggcacggcgt tcggggcggc
      181 aggctcaatc gtgatcatcc tggtgtgggt ttactactca tcgctcatct tcttcatagg
      241 ggccgagctg acccaggtct acgcccgccg cttcggttcg cgagtggtgc ccgactccaa
      301 cgccgagcca ctcaccgacg aggcccgcgc ccaggaaggc atccccaaga aggacggcga
      361 cgataaggac cgcacaggcg gcggtgacgg caagtcgcgc ccgaagcacc gcagcctgaa
      421 agcctccccc tggttcagat agacggccat agtaacgcgt aacacgtaac acgtaagagg
      481 acggtgggtg ggaagacagc ggctacaaag gtaaatagcc gccatagcca ccttacgtgt
      541 tacgcgttac gtgttacgcg ttatccccaa agaggtcacc ccatgaaagg caattcgctg
      601 cttggcctgg tcaaggagac cttccaggac tggacccagg acaaggcctc gcgcctgggt
      661 gcggccctgg cctactatgc gatcttctcg attgggccga tgctggtggt ggttattgcc
      721 atcgccggta aggtattcgg caaggacgcc gccgagggga agatctcgga cacgctaagc
      781 caggtgctct cctccgacgc tgcaaagttc gtggaggcgt tggtgacgag cgccagcaag
      841 cccaaggagg gcaccatagc aaccgtagtg ggcaccggca cgctgctgct gagcgcgatg
      901 ggcgtattcg gccagctaaa ggacgccctc aacacgatct gggaggtcga gccggtgccg
      961 ggcggcggga taatggccgc gctcaccaag aacttcctgt cgttcggcat gctgctgggg
     1021 gtcggtttcc tgctgctggt gtcgctggtg gtaaacgcgc tcctctccac gctggggccg
     1081 ttcctcagag atgccctacc gggcggggcc ttgctctgga acgcggtcaa ctacgtgatt
     1141 accctggcca tcataagcct gatgttcgcc ctcatcttca agttcgtgcc cgacatccgc
     1201 gtaggctgga aagaggtgct tttaggtggg gtcgtgaccg ccacgctctt cctgctgggg
     1261 cagatagccc tgggtatcta cctcagcctg ggcaacgtcg gctcggcgtt cggggcggcc
     1321 gcctccctgg tcatcgtgct cgtgtggatc tactactcgg cgcaaatcct attcctgggg
     1381 gccgagttca cccaggtcta cgccaaccgc tacggctcag gtatggcccc cggccgccac
     1441 gcccgcctcc tcaccgagga ggcccgcgcc caacagggca ccaagagcaa gcagccccgc
     1501 aagcaagcag cccgcgagca ggaggccgaa cccggctccc ccacccacaa gacctccccc
     1561 tggttccgct acgtgagcca tgtagagacg gaactgccaa gacgccaagc acgcgccaag
     1621 gaccacggca agaggatgaa gggtttgggg tgtaggggca ctatgcatca agctcctacg
     1681 gccatatgct tgtgccgaac gcgtgggaca gtattccccc ttgtcatcct gagcgtagcg
     1741 aaggatcagc cgcccgttct tgatcctctc tattgctgca ccgctgttcc ccatgccacc
     1801 agcagccaga aagttgaaga ctctggccta cccacctgat ccttcgctac gctcaggatg
     1861 acaagggggg cttggtccca tagaacgctg tctaccggtt accaaccaga gtaatatgca
     1921 tagggcccgt acgccccaaa ccattaatta tcttggcgtg gtcctttgcg ttctttgcgt
     1981 ctttgcgttt tcgtctcttt aacacatacg gagagtgtcg aacgtgggaa gctgtagtac
     2041 aataaatggc tatgtcagac aagattagta tcgtgcaggg cgacatcaca aagctggcgg
     2101 tggatgccat cgtgaatgcg gccaacaatt cgctgctcgg cggtgggggc gtggacgggg
     2161 cgatacatcg cgccgccggg ccgaagctgc tcgcggagac ccgcacgctt gggggctgcc
     2221 ccaccgggga ggcccgcatc acgggtggct acaaccttcc ggcgaagtgg atcatccaca
     2281 ccgtggggcc ggtctggcgg ggcgggaacg ccgaggagga cgcgctgctc gcttcggctt
     2341 accgcaacag cctcaagctg gccggcgaac atggcgcgcg cacgattgcc ttcccgtcca
     2401 tcagcaccgg cgcgtatggc ttcccggtag agcgggccgc ccgcatcgcc ctgcgcgaga
     2461 tgagcgactt cctggccgcg gggccgcgct cggtggagca ggtaatcctg gttgcctacg
     2521 gcgagcgtac atataaggcg tacacagaag ctctagagga gatcgatgct tagcatgcag
     2581 gccttcatct ttgacatgga cggggtgatc gtggacagcg agctgcactg gaagaagccg
     2641 gaaggttact tcctgcgctc cctcgtgccc aactggaccg atgccgacca gacgcgcata
     2701 atcggcctga gcctgagcaa cgtgtaccgc atgctcacta cggagtacgg ggtaacgcac
     2761 agcaaagagg agttcctcga agtctacatg gctatggcgc gccagattta tggcgagttg
     2821 tcgtcgctga tgcccggctt cctcgtcctg ttgcgggaat tgcaccggcg aggcactccc
     2881 gttgccgtag cctcctcctc cacccgcc
//
LOCUS       DASRWZ010000427        13730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_790278, whole genome shotgun sequence.
ACCESSION   DASRWZ010000427 DASRWZ010000000
VERSION     DASRWZ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 13730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13730
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790278"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VFH60_06645"
     CDS             complement(<1..1139)
                     /locus_tag="VFH60_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313496.1"
                     /translation="MDIRDRVMGDQPNDFLHKIAGIIPGYKGYVDRERRRDADRLLRT
                     ELARRYQAELTRIKRVQQDLLRSGDLNTIGELDGISGRLQRFIDRLQNATYGYTGLFA
                     PMKVEAEELDQIYAFDMALTSGVDTLAGAIGALESGIDGDTNAKLRPAVDNLSTVVND
                     LNDRFDQRADLLTAGTTLPQNEYDAMVGGFQQPSQGGQGYPQPAQPESPSPYAQQAAN
                     ITSTSGTGSAGTHGNQGGGYGGGTPTAALGSTARVATTGQAGIAGSGASLDDAESPTT
                     TGPIATAAVGQDHNMLPVDDPSIASGGTPTTRVSWGSSSAMQPDPAVDSMAQGNQGFG
                     GTPTGTDQGGSAPMTLGGEAGSGIESASSPAAAPGAPGGDIVDSM"
     gene            complement(1298..3301)
                     /locus_tag="VFH60_06650"
     CDS             complement(1298..3301)
                     /locus_tag="VFH60_06650"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET6313497.1"
                     /translation="MLNPLELLRGALGPRHGRRSLAMWLLLSVCVTAALAVAPNTTTA
                     QERQYHMDRYDSRIVVNTDGSFDIEETLVYVFQGQYRRGLREWDTDKLENIDGFEVFE
                     LVNGEEVPYRETTFAPDEPSTSGTPGTFGVRNGNPTRVRWVYAGGPTPTSGTTEKTFI
                     IRYHVDGALRVYADRDELDWYAVPPRWGAPINRSRVEVVLPEGTDTSAFKTLAIPRDA
                     EVTKQGDTIVYTKQGGLDDGFEVLAQVPKGILSATQPSWQPAVDAIEREQAEQQAAID
                     RVKPLLDFGLLVLSLLVGVGGVLFVVRRWYTKGRDKPVKLFSDYVAEPPSNLPPGLVG
                     TLLDESADVRDVIATVVDLARKGSITIQETRTPGFLTQGKDFEYRLLNNKVEYRYEEM
                     LLGAMFTGTSVASLSSMKNTFYSKLPPIYEEMYKSLVALKYFPENPKAVRSRNMGAGC
                     GVAVLGGLILFLSIAIAPAFTSMLPLLGVAIGAVGLVWVFTSSAMPRKTDFGAEETAK
                     WRAFSSYLQEIQRYTDVQAAADKFQHYLPYAVALGIERQLINQFNNVPAAMPPYYAPY
                     GWHPYGYYPVGASGPAQQQAGGAPGGPAPNFDPGGAMQGMSDSLGGAMQGMADSFTTM
                     VNSASSALTSQPSSSGSSGGGGWGGGGGGGGGGGGGGGGGGAD"
     gene            complement(3348..3947)
                     /locus_tag="VFH60_06655"
     CDS             complement(3348..3947)
                     /locus_tag="VFH60_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313498.1"
                     /translation="MVGSGRVIGLVLIGVAVVMFLGAGIFVFGQMNQTNTQMQAGGAA
                     LTMGCALLVAAVVAGFGVWFLMQGRSEAGQFADVEKQKRILNIIQTQGTVQLGTVALE
                     TNMPMDQVKSAIYDLVGKGLFTGYVDWKSGKLVSSDAAAITTAAISGKCPNCGAPQVV
                     GGKGVIRCEYCGTEFFLPPAEQRAAAQAAEAAHPPTTPQ"
     gene            4688..4972
                     /gene="tatA"
                     /locus_tag="VFH60_06660"
     CDS             4688..4972
                     /gene="tatA"
                     /locus_tag="VFH60_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009656386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET6313499.1"
                     /translation="MNFSGPEILLVLVIVLIIFGAGKLPQVFSSLGKGVREFRDAAEG
                     DPTAPTTPTTSTGATTTPTTAAPTTPIAPIAPVAPTAASTTTTEEATRQG"
     gene            complement(5065..6153)
                     /locus_tag="VFH60_06665"
     CDS             complement(5065..6153)
                     /locus_tag="VFH60_06665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET6313500.1"
                     /translation="MHVAGDPKRILLLMLLPIGDTLFTTPSVAALRRRYPEAHITALV
                     YPTNRGILSNNPDVDDFLLWPTRQRWPGAGGVLKLFRGLRRARFDLAVEFSNYNWWVS
                     WLSRIPRRTEMQLPRFWWALPWAGLEWRTHHAVEHYTGPVRRLGIPVEDTALRFFPSV
                     QDEAKASAWLERHKVGPDELLVGIHPGGEGLWGRKRWSVARFAEVADGLSARLGARIV
                     VLGGKDDVPAAAGIAARTGASIINATGQTTLAETAALVRQCALFIGNDSSPLHIAAAS
                     GTPVVGIYGPTDPRSYRPWIPGGEEGVDYAVVRSDLPCACKFPLVGGTTLAGWVTCLN
                     CPALESITPQQVLDAALRLLEGRVRRNS"
     gene            complement(6369..6620)
                     /locus_tag="VFH60_06670"
     CDS             complement(6369..6620)
                     /locus_tag="VFH60_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313501.1"
                     /translation="MAKVVIIHVIGEDPILADMEELPKPQDLFVEFTNPRKRDGKAVA
                     YMTHGAKVFMYPWHRLSFIEVMTTETEREEVIEFFREDR"
     gene            complement(6759..8930)
                     /locus_tag="VFH60_06675"
     CDS             complement(6759..8930)
                     /locus_tag="VFH60_06675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6313502.1"
                     /translation="MLIKRLEGVQAAKNGTGPTTEESQAAGAGQTSPYASPARSAHNT
                     EVMPGTGTAPLPRNTVLQGRYDVEEVLGVGGMSTVYKARDLRFTAVTRYCAVKEMPDN
                     SPDIRTGQIRLANFEREASLLATLSHPGIPKIYDFFSVHGRVYLVLEFIDGRDLESQL
                     NNKEHPMLEEEVVRLAVQICDVLEYLHGHSPQPIIFRDLKPSNIIVQPDGKLTLIDFG
                     IAKVFQSDKRGTMIGTEGYAPPEQYRGLAEPRGDIYGLGATMHHLLTKSDPRIQTPFT
                     FHERPIRKFNPRVSEYLESIILQAVEYDIDRRWPSAPAFRQALMQTSVLDPKLKKRQS
                     GPLGDDQAPLIDSAPYPAGSGSAGNSNAMMRPDVSSGVLLPPSYAASKDRASAADKAS
                     TPAAMGVGADGAQVLWTFTADDEIRSSPLCSRGVVFFGAYDTNLYALNAKSGDFLWKA
                     PTKAGICSSPTLVNDLVIVGSEDGYVYAFDARRGTTSWIYRTGSPVRSSPKVYKDMLF
                     IGSDDQNIYALQADTGQIMWKYRTWNHVRSSATVANGLVMIGSSDGNLYALDTLSGTL
                     KWKMHTLGAIISTPGIAGNTVIVGSLDNYVYGVDADTGFTSWRFETQRSVSSSPAVMG
                     ERVLIGSTDGIMYALDAHSGRTIWDFDTQSQITSSPKVSPVSDSVYFGTVNNYIYSLD
                     LITGEQQWRYRTRGPVVSSASVEDGIVYIGSLDRKLYALAI"
     gene            9157..9921
                     /gene="lepB"
                     /locus_tag="VFH60_06680"
     CDS             9157..9921
                     /gene="lepB"
                     /locus_tag="VFH60_06680"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET6313503.1"
                     /translation="MRIEDDKYWREAARLPYSDDGDLGDDYEDNGAYPGGSRPSVVRE
                     VIETILVTLLIFFVVRSVVQNFKVEGDSMLPTLHSEQYLLVNKGLYFRYDTNFMSRLT
                     NPDTPTDMRYLFHGPQRGDIVVFESWHEPRDFIKRVIAVEGETIEVRPEPGMTDLPPN
                     DCDGCCSACDVYVNGIKLNEPYIHERPNYKRAPIVVPPGQVYVLGDNRRNSSDSHVDI
                     NGPLPVEKIVGTAFVSYWPQDRWGFLPHPTYAEMDK"
     gene            10135..11112
                     /locus_tag="VFH60_06685"
     CDS             10135..11112
                     /locus_tag="VFH60_06685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6313504.1"
                     /translation="MMKRYPVVSFFALAYIITWSFQIVSYLIALNAGVSLSNEDNFVH
                     FSDLLTFRISGDRLVPFLIYSIGQFGPVLAAFIVTSAVYGRVGVRDLAGRILKWRVPS
                     RWYLTVLLLPLFLAAVSLGAALVSGGFTLGPFSPVLSWLYFVPFLLFMLVFTGLAEEP
                     GWRGFALPHLQAKYSAIRSTWIVGIFWGLWHLPFAVYYNREQPALLIPALFGLTLGIV
                     GAAIVYSWVYNSTGSVFLLILLHGWENTVRSYLILSQPNALAIALYNLVPWAIAIYLA
                     KRYGDENLGREPRPKWWPGKYNTQQRGEATEQSRAVEPTTAPPQAASAN"
     gene            11973..>13730
                     /locus_tag="VFH60_06690"
     CDS             11973..>13730
                     /locus_tag="VFH60_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313505.1"
                     /translation="MPAASEGAEEGATSERVTALGGIQRGARVALAERVGALAAFRRW
                     YWSLVPLLTIAAYATVVRIGFLSEDMGLNRIAQGEVGWQALLPNDGLRFYRPLGFLLT
                     WRINWPLFGLNPFPYHLEGLLVHAAAAWVVGLWLADITGKRWLGWLAGVLFGVFPLNM
                     EAVGWLATQWDLWAALFGTLSVWQFSKWWRGDGRDRRDRRDGRGRRGLYIGSVLFYFL
                     GIFTKESLFAFIPIIALSAWYVLPPNSPGKWGRLGLALVPHTALLGLNFLIRQIAWGE
                     QRNYTGVDINYLNFLYDWPVDRMRFLLAPIESIIFGEPAVQVVAALSALLLLVGLVLH
                     GRDLARLLLLAGAWMALTLVPVLNIGIFRHDFQNSRLLYLVSAGYCIVVAALLWSTIS
                     QAKRARWPLISGTGMLIVACVVVTWIQLHPWHTASVQATEVESELARLIPMQERPQGM
                     VWYLNNVPDRYKGAWVLNSAMSRTRYLPGGAPDIPDVVRVDEVENVPLGSDGRDSFAL
                     RWEFDPAGPRWHIHYGLGITRDSDPPDLDNNTGSNLLLWDFRGCDAAVLMQWEVINAS
                     ASCAPGKGLLLKPANEDPQV"
BASE COUNT         2376 a         4201 c         4394 g         2759 t
ORIGIN      
        1 tccatgctgt ccacgatgtc gccgccgggc gcgcctgggg ccgcagccgg ggacgaggcc
       61 gactcgatgc cgctaccggc ctccccgccg agcgtcatag gggcgctacc gccctggtcg
      121 gtgccggtgg gggtgccgcc gaagccctgg ttgccctggg ccatgctgtc caccgccggg
      181 tcgggctgca tggcgctgct gctaccccag cttacccgcg tggtgggcgt gccgccggac
      241 gcgatgctgg ggtcgtccac gggcagcatg ttgtggtcct gccccacggc ggccgtggcg
      301 atggggccgg tggtcgtggg cgactccgcg tcatccagcg aggcccccga accggcgata
      361 cccgcctgcc cggtagtggc tacccgcgcc gtcgagccga gagcggccgt cggtgtgccg
      421 ccgccgtagc ccccgccctg attaccgtgg gtgcccgccg agcctgtgcc ggaggtgctg
      481 gtgatgttgg cggcctgctg cgcgtagggg gagggggatt cgggctgggc cggctggggg
      541 tagccctggc cgccctgtga cggctgctgg aagccgccaa ccatcgcgtc gtactcgttc
      601 tgtgggagag tcgtgcccgc cgtgagtagg tccgccctct ggtcgaagcg gtcattgagg
      661 tcgttcacca cggtggagag gttgtccacg gcaggacgca gcttggcgtt cgtgtcgccg
      721 tctatacccg attccagggc accgattgcc ccggcgaggg tatccacccc gctggtgagg
      781 gccatatcga aggcgtaaat ctggtccagt tcttccgcct ctaccttcat cggagcgaac
      841 aggccggtgt agccgtaggt ggcgttttgc aggcggtcga tgaaacgctg gagcctgccg
      901 gagatgccgt caagctcgcc tatcgtgttc aggtcgccgc tgcgcaggag gtcttgctgc
      961 actcgcttga tgcgggtaag ctcggcctgg tagcggcggg ccagctcggt gcgcaggagc
     1021 cggtcggcgt cgcgccgcct ctcccggtcc acgtagccct tgtagcccgg tatgatgccg
     1081 gcaatcttgt gcaagaaatc gttgggctgg tcgcccatca cgcggtctct tatatccatg
     1141 cgtttctctc cttcttagtg atgaggactg aggactgagg actgagttat ttcaggccgg
     1201 tccgagtctg ccgtcagtag ctacgttgtc acgaggtcca aggttgcgct taccgccggg
     1261 acctgctcag tcctcagtcc tcagtcctca ttactcccta gtccgcgccg ccgccaccgc
     1321 cgccaccacc tccgcctccg ccgcctccgc caccaccgcc ccagccgcca ccaccgctgc
     1381 tgccgcttga ggacggctgg ctggtgaggg cgctggacgc ggagttgacc atcgtggtga
     1441 agctatcggc catgccctgc atagcgcctc ccagcgagtc gctcatgccc tgcatggcac
     1501 cgcctggatc gaagttgggt gcggggccgc ccggcgcgcc acctgcttgc tgctgtgcgg
     1561 ggccgctcgc cccaacagga taatagccat aagggtgcca gccatacggt gcgtagtagg
     1621 gcggcatggc ggcgggcacg ttgttgaact ggttgattag ctgtctctcg atgccgagag
     1681 ccaccgcgta gggcaggtag tgctggaatt tgtcggcggc tgcctgtacg tcggtataac
     1741 gctgtatctc ctgcaggtag ctggagaagg cccgccactt ggccgtctcc tcggccccga
     1801 agtcggtctt tctgggcatg gcgctcgacg tgaacaccca caccaggcca accgccccga
     1861 tagccacgcc cagcaggggc agcatggagg tgaaggccgg ggcgatggcg atgctcagga
     1921 agagtatcaa gccccccagg acggccacgc cgcagcccgc ccccatgttg cggctgcgca
     1981 cggccttggg gttctcgggg aagtacttga gggccaccag gctcttgtac atctcttcgt
     2041 agatgggcgg gagcttgctg tagaaggtgt tcttcatact cgacaggctc gccacgctgg
     2101 tgccggtgaa catggcaccg agcagcatct cctcgtagcg atactccacc ttgttgttca
     2161 acaggcggta ctcgaagtcc ttgccctgcg tgaggaagcc gggggtgcgc gtctcctgta
     2221 tcgtaatgct gcccttgcgg gcgaggtcca ccacggtggc gatcacgtcg cgcacgtcgg
     2281 cggattcatc gagcagggtg cccacgaggc cggggggcag gttgctgggc ggctcggcca
     2341 cgtagtcgct gaagagcttg accggcttgt cgcggccctt tgtgtaccag cggcggacca
     2401 cgaagagcac gccgcccaca cccacgagca agctcagtac cagcaggccg aaatcgagca
     2461 ggggcttgac gcggtcgata gcggcctgct gctcggcctg ctccctctct attgcatcga
     2521 cggcaggctg ccacgagggt tgtgtcgcgc tcaggatgcc cttgggcacc tgcgccaata
     2581 cctcgaagcc atcatcaagc ccgccctgct tggtgtagac gattgtgtcg ccctgcttgg
     2641 ttacctcggc gtcgcggggt atagccagcg ttttgaaggc actcgtgtcg gtgccttccg
     2701 gcaacaccac ttccacccgg ctcctgttga taggtgcgcc ccagcgcggg gggaccgcgt
     2761 accagtcgag ttcatcccgg tctgcgtaga cgcgcagggc accgtccacg tggtagcgga
     2821 tgatgaaggt cttttcggtc gtgcctgaag ttggggtcgg cccgcccgcg taaacccagc
     2881 gcacccgcgt ggggttgccg ttgcggacgc cgaaggtgcc gggggtgccg ctggtggatg
     2941 gctcgtcggg ggcaaaggtc gtctcgcgat agggcacctc ttccccgttc accagctcga
     3001 acacctcgaa gccgtcgata ttttccagct tatcggtgtc ccactcccgc agaccgcgcc
     3061 ggtactggcc ctggaagacg taaacaaggg tctcctctat gtcgaaggat ccgtcggtgt
     3121 tgaccactat ccttgagtcg tagcggtcca tatggtactg ccgttcctgg gcagtagtcg
     3181 tattgggggc gacggcgagg gcggcggtga cacacaccga gagcaggagc cacatggcca
     3241 gggagcgccg cccatgtcgc ggacccagcg cgcccctgag aagttcgagc ggatttagca
     3301 tcagactacc tttgtattag agactgctat gcgagagcca tacatgatta ctgtggggta
     3361 gtgggcgggt gggcggcttc cgcggcctgg gcggcggcgc gctgctcggc gggcggcagg
     3421 aagaattcgg tgccgcagta ctcgcagcgt atgacgccct tgccgccgac cacctgggga
     3481 gcgccgcagt tggggcactt gccggagatg gcagcggtag taatggcagc cgcatcggac
     3541 gacacgagct tgccactctt ccagtccacg tagccggtga agaggccctt gcccacgagg
     3601 tcgtatatgg ccgacttcac ctggtccatc ggcatgttag tttcgagggc cacggtgcct
     3661 agctgcacgg tgccctgggt ctggatgatg ttgaggatgc gcttctgctt ctcaacgtcg
     3721 gcgaactgcc cggcctcgga gcggccctgc atgaggaacc agacgccgaa cccggccacg
     3781 accgccgcga ctagcagggc acagcccatc gtgagggctg cgcccccggc ctgcatctgg
     3841 gtgttggtct ggttcatctg cccgaacacg aatatgcccg cgcccaggaa catgaccacg
     3901 gccacgccga taagcaccag gccgattacc ctacccgaac caaccatgca tacacctccg
     3961 ctcatctagc cgcatgccac cgggcggctc acccgtcact atatacgtgc gtacccacaa
     4021 aacgagtttc atgccgcgcc cacacgcaca ctaaagtggc gctcgcccac gcacaaacag
     4081 acaatgcagc ccatcttaca acataatgcc actcttgtct attgagttct atagagacaa
     4141 aaatagacat aggcaaatag acgaaaacgc aaagacgcaa agacgcaaag gacgcgcaaa
     4201 gaggatgatg gtttggactg gatgcggcat gcagcatcgt gccagagtgg ataaccccta
     4261 accggcattg atgggccaga gtccctacct tgtcatttcg aacgtagtga gaaatttcgt
     4321 ccttcatgca gatgctcccc ctttcttgta ggaagctcat atagaccgtc ctgaaagaaa
     4381 agtggggttg ggtgctgagg gacgagattt ctcactacgt tcgaaatgac aaaagcgggg
     4441 cttggtcctc aatatgcggt acaggtgctt accacgggga gcacggtgca tggtgctcct
     4501 acaccccaaa cgatccttga ctttgcgcgt cctttgcgtc tttgcgtctt tgcgttttcg
     4561 tcttaatctc gatagcaatg tggctactat tggtgcgtgg tggtataatt ggcgcaaagg
     4621 cacgtgatac tagaactttc cagcggcagt gcgcgaggcg tagcaggtcg caagaggaga
     4681 tgaggccatg aattttagcg gacccgagat actgttggtg cttgttatcg tactgatcat
     4741 cttcggcgcg ggcaaattgc cgcaggtgtt cagctcgctc ggcaagggtg tgcgcgagtt
     4801 ccgggatgcc gctgaaggtg accccactgc cccgacaacc ccgaccacct ccaccggcgc
     4861 gaccacaacc cccaccacag cagccccaac cacccccatc gcacctatcg cccccgtggc
     4921 ccccactgcc gcctcgacca caaccacgga ggaagctacc aggcagggat agagtgccgg
     4981 gtaacgcgta acgcgtaaca cgtaaagcgg ctccagggac tttgttccct aagagccgct
     5041 ttgttgctta ttgcacccta cgagttacga gttacgtctt actctcccct ccaggaggcg
     5101 cagggccgcg tccagcacct gttggggggt gatgctttcg agggccgggc agttgaggca
     5161 ggttacccag cccgcgagtg tggtcccgcc taccaggggg aacttgcagg cgcaggggag
     5221 gtcggagcgc acgacggcgt agtctactcc ttcttcgccg ccgggtatcc agggccggta
     5281 gctgcgcggg tcggtggggc cgtatatgcc caccacgggc gtaccagagg cggcggctat
     5341 gtgcagcggg ctggagtcgt tgccgatgaa gagcgcgcat tggcggacga gggcggcggt
     5401 ctctgccagt gtggtctgcc ccgtggcgtt gatgatagag gcgcctgtgc gggcggcaat
     5461 accggcggcg gcgggcacgt cgtccttgcc gcccaggacc acgatacgcg ctcccagccg
     5521 ggcggacagg ccgtcggcca cctccgcgaa gcgcgccacg ctccaccgct tgcggcccca
     5581 cagcccttcg ccgccggggt gtattcccac aagcagctcg tccggcccta ctttgtggcg
     5641 ttcgagccag gcgctcgcct tcgcttcgtc ctgcacgctc ggaaagaagc gcagggccgt
     5701 gtcttccacc gggatgccca gcctgcgcac cggcccggta tagtgctcaa ccgcgtggtg
     5761 ggtgcgccat tccagcccgg cccacgggag cgcccaccag aagcgcggaa gctgcatctc
     5821 ggtgcgccgg gggatgcggc tcagccagct aacccaccag ttgtagttgg agaactccac
     5881 agccaggtcg aaccgggcac ggcgcaggcc ccggaagagc ttgagcacgc ccccagcgcc
     5941 gggccacctc tgccgcgtcg gccagaggag aaagtcgtct acatcggggt tgttgctgag
     6001 gatgccccgg ttggtcgggt agaccagcgc ggttatatga gcctccgggt accgcctccg
     6061 cagcgcggct accgagggcg tggtgaagag cgtgtcccct atcggcagga gcatcagcag
     6121 gagtatgcgc ttggggtcgc ccgcgacgtg cacggcgtca ttctacacta catacaccac
     6181 caggcgatag ggcatgagac gaaaacgcca agacgcaaag aacgcaaagg acgcgcaaag
     6241 tttaaggatg gttgcgtggt acacagagga ccagggtacc acagcggtac cttggtcctt
     6301 ttgctcagaa caacctctta accttggcgt ggtccttggc gttcttggcg tcttggcggt
     6361 ttcgtctctt accgatcttc gcggaagaac tcgattacct cctcgcgctc ggtctcggtg
     6421 gtcattactt cgatgaacga gaggcggtgc caggggtaca tgaacacctt ggccccgtgg
     6481 gtcatatagg cgaccgcctt gccgtcgcgc ttgcgcgggt tggtgaactc cacgaacagg
     6541 tcctgcggct taggaagctc ttccatatcc gccaggatcg ggtcttcacc gattacgtgt
     6601 atgattacta cctttgccat tgctctcctc ggtgttacga gtaatgagtg atgaggactg
     6661 aggactgagg actgaggact gaggactgag gactgaggac tgagttactc ggatgcctgc
     6721 tgacaactca taactcagtc ctcatcactc attactcgtt agatagccag tgcgtacaac
     6781 ttgcggtcca acgaacctat gtagacgatg ccgtcctcca cggaggcgga cgaaacgacg
     6841 gggcctcgcg tgcggtagcg ccattgctgc tcgcccgtaa tcaggtctag cgagtagatg
     6901 tagttgttta cggttccgaa gtagacgctg tcgctcacgg gcgagacctt gggcgacgac
     6961 gttatctggc tctgggtgtc gaagtcccag atggtgcggc cggagtgggc gtcgagggcg
     7021 tacatgatgc cgtcggtgga gccaatcagc acgcgctcgc ccatcacggc aggcgacgac
     7081 gagaccgagc gttgcgtctc gaagcgccag gaggtgaagc cggtgtccgc gtccacgccg
     7141 tacacgtagt tgtcgagcga gccgacgata acggtgttgc ccgcgatgcc gggcgtgctg
     7201 atgatagccc cgagcgtgtg catcttccac ttgagcgtac cgctcagcgt gtcgagggcg
     7261 tacaggttgc cgtcgctgga gcctatcatc acgaggccgt tggccaccgt ggcggaggag
     7321 cgcacgtggt tccaggtgcg gtacttccac attatctgcc ccgtgtcggc ctgcaacgcg
     7381 tagatgttct ggtcgtccga gccgatgaag agcatgtcct tgtacacctt gggcgacgag
     7441 cgcacgggcg agccggtcct gtagatccag gaggtggtgc cgcgcctggc gtcgaaggcg
     7501 tacacgtagc cgtcctccga gccgacgatt accaggtcgt tcaccagcgt gggcgacgag
     7561 cagataccgg ccttggtggg ggctttccag aggaagtcgc cgctcttggc gttgagggcg
     7621 tacaggttgg tgtcgtacgc gccgaagaag accacgcccc ggctgcacag gggcgaggag
     7681 cgtatttcgt cgtccgccgt gaaggtccac agcacctgcg cgccgtccgc gcccactccc
     7741 atagcggcgg gcgtgctcgc tttgtcggcg gcgctggccc tgtcttttga cgctgcgtaa
     7801 ctaggcggca ggagcacgcc gcttgacacg tcggggcgca tcatagcgtt gctgttaccc
     7861 gccgagccgg agcctgccgg gtagggggcc gagtcgataa gcggtgcctg gtcgtcgccg
     7921 agggggccgg actggcgctt cttcagcttg gggtcgagca cgctggtctg catgagcgcc
     7981 tggcggaagg ccggggccga gggccagcga cggtcgatgt cgtactccac cgcctgcaag
     8041 atgatcgatt ccaggtactc gctgacgcgg gggttgaact tgcgtatggg ccgctcgtgg
     8101 aaagtgaagg gcgtctggat gcgcgggtcc gacttggtca gcaggtggtg catggtagcg
     8161 cccagcccgt agatgtcgcc tcgcggctcg gcaaggcccc tgtactgctc gggcggggcg
     8221 tagccctcgg tgcctatcat cgtgccgcgc ttgtccgact ggaacacctt ggcgataccg
     8281 aagtcgatca gggtgagctt gccgtcgggc tggacgatta tgttggaggg cttgaggtcg
     8341 cggaagatga tcggctgggg cgagtggccg tgcaggtatt cgagcacgtc gcaaatctgg
     8401 accgccaggc gcaccacttc ctcttccagc atcgggtgct ctttgttgtt gagctgcgac
     8461 tccaggtcgc gcccgtcgat gaactcaagg accaggtaca cgcgcccgtg gaccgagaag
     8521 aagtcgtaaa tcttgggtat gccggggtgg gacagggtgg cgagcaggga ggcctcgcgc
     8581 tcgaagttgg cgaggcgaat ctgcccggtg cggatgtcgg gcgagttgtc gggcatctcc
     8641 ttgaccgcgc agtagcgggt gaccgccgtg aagcgcaggt cgcgggcctt gtacacggtg
     8701 ctcataccac cgacgcccag cacttcctcc acgtcgtacc ggccctggag caccgtgttg
     8761 cggggcaggg gcgcggtgcc ggtgcctggc atgacctcgg tgttgtgggc ggagcgggcg
     8821 ggcgaggcgt aagggctggt ctgccctgcc cctgcggcct ggctttcctc ggtggtgggg
     8881 ccggtgccgt tcttggctgc ctgcaccccc tctaaacgct tgatgagcat ggcgtggttc
     8941 tcccgtaata ttgctggact ctaattagat tataggtttg ggcacggggt agggtaaata
     9001 gccctaatgt cccatagggt atgggctatt ggtatgcgct ttggctttgt aatttgatgt
     9061 ggctaccgga tataatgagg tcatgtgctc gtgcaccctg tctatcaatc gccgcgcagg
     9121 cgcggcccaa agaatatgat tgcgatatga gagcagatgc gtatagaaga cgataagtac
     9181 tggcgagaag cagcccggct accttactcg gacgacggcg acttaggcga cgattacgag
     9241 gacaacgggg cttatcccgg cggctcgcgg ccgagcgtgg tgcgcgaggt aatcgaaacg
     9301 atactggtta ccctcctgat tttcttcgtg gtgcgctccg tagtgcaaaa cttcaaggtc
     9361 gagggcgaca gcatgctgcc cactctgcac agcgagcagt acctcctggt gaacaagggc
     9421 ctctatttcc gctacgacac caacttcatg tccaggctga ccaaccccga cacgcccacc
     9481 gatatgcgct acttgttcca cggcccccag cgcggcgata tcgtcgtgtt cgagtcttgg
     9541 cacgagccgc gcgacttcat caagcgggtg atagcggtgg aaggggagac gattgaggtc
     9601 aggccggagc cggggatgac ggacctgccg cccaacgact gcgatggctg ttgcagcgcc
     9661 tgcgacgtgt acgtgaacgg catcaagctg aacgagccat acatccacga gcggccgaac
     9721 tacaagcgcg cgcccatagt ggtgccgccg gggcaggtct acgtcctggg cgacaaccgg
     9781 cgcaactcca gcgactccca cgtcgatatc aacggccccc ttcccgtgga gaagatagtg
     9841 ggcacggcgt tcgtctctta ctggccgcag gacaggtggg gcttcctccc gcacccgacg
     9901 tatgccgaga tggacaagta gggcgcgcgt tcaaatgtag cgggctgcaa acatcacgcg
     9961 cgagtgataa cttgagagtt tagagagcac agccatgatc tggcaaggtc gatcatgcct
    10021 gtgctctttg tatttgtttg acctgtaata ccccacgtca aaagagggta ttccaaaaag
    10081 caaatgccta tgatataatc ctcgaagttt aatatacctg ggggcgcgca ccgtatgatg
    10141 aagcggtatc cggtcgtgag cttctttgct ctcgcctata taatcacgtg gtctttccag
    10201 atagttagct acctgattgc ccttaacgcc ggggtctctc tcagcaacga ggacaacttc
    10261 gtccacttct ccgatctcct tacgtttcgc atctccggcg accggctagt acccttcttg
    10321 atctacagca ttgggcagtt cggccccgtg ctggctgcct tcatcgtcac atccgctgtg
    10381 tacggtcgtg tgggcgtgcg ggacctggcg gggcggatat tgaagtggcg cgtcccctcc
    10441 cgctggtacc tgacggtgtt gctgctgcct cttttcctgg ccgccgtatc actgggagcg
    10501 gcattggtca gtggcggctt cacactgggt ccgttctcgc ccgtgctctc ctggctctac
    10561 ttcgtgccct tcctgctctt tatgctcgtc ttcacggggt tggcggagga gccgggctgg
    10621 cggggcttcg cgctccctca tctgcaggcc aagtactctg ctatccgttc aacctggatc
    10681 gtgggtatat tctggggcct ctggcatctc ccgttcgcgg tctattacaa ccgggagcag
    10741 cctgctctgc ttatcccggc cctattcggg cttaccctcg gcattgtcgg cgcggcgata
    10801 gtgtactcgt gggtgtataa cagcaccgga agcgtgttcc tgttgatcct gctacatggc
    10861 tgggaaaaca cggtgcgctc gtacctcatc ctttcgcagc ccaatgccct ggcgatagcg
    10921 ctgtacaatc tcgtgccctg ggcgattgct atctacctgg ctaagcgcta cggggacgaa
    10981 aacctcggac gtgaaccccg gcccaagtgg tggcccggca agtacaacac ccagcagcga
    11041 ggcgaagcaa ccgagcaaag tagggcagta gaacccacaa ccgcgccccc tcaggcagcc
    11101 tcagcaaact aggtttgctt ctaactggaa caacccggca actaagaact catttcaaaa
    11161 cctggaagat agaggttgta ggggcaggcc cccgtgcctg ccctggtgcc aatgtccaac
    11221 aaaggcaggc acgggggcct tcccctacaa ggggttttga aatacgctct tagtcatgat
    11281 cctgtatacg gatgatgacg ctacaacgtt gaagtgaaat gcttaccggc tctggttcgc
    11341 caccttgctg atgatgagct taaccatacg ccctactcgt cccttgccag ccttgacctt
    11401 cgttggccgg cgaacgatgg accaagtgaa cttgattgtc accgactggc ttgcctttct
    11461 cggccgctgc aagatgtgta tcctcgtctc gctaatgggt cactaagact acgccgtaaa
    11521 ctccctgcgc cagcgattga gtcacttctt agcgttcaca acccttggtc tgaccgtctt
    11581 tcgaaagcta ttgatgcacg ccctggtagc ttcgagcgct atgctatcgc gctcgtggcc
    11641 tgattggggc gcaatcaggc cacaaatggg gtgggttgta taacgcacca ccgcttttta
    11701 ctgcatctca gacgccgccc ggcgacttgt gttagcttgc cataattggg cgtggactgg
    11761 cgaccatgct gctcatactt gcatcacagg ctgttggcgg ccatcccaca cgggctatgc
    11821 ttgtcaattt gcatcagctc caagccgtct tcgtaccccc ctaccgcgaa tgaccacacc
    11881 ctgagcgagt gcaaactaca tattgaagtg gtaccgctcg ttgtgctatg ctcatggtta
    11941 tgagttcatc cacgctggat aaacccaggt cagtgcccgc cgcctctgaa ggtgccgagg
    12001 agggcgcgac ttccgagcgc gtaacggcgc ttggaggtat ccagcgtggc gctcgcgtgg
    12061 cccttgccga gcgggtgggc gcgctggcag ctttccggcg ctggtattgg tctctggtgc
    12121 ccctgctcac tatcgccgcc tacgccaccg tggtgcgcat cggcttcctt tcggaagaca
    12181 tggggctgaa ccgcatagcg cagggggagg tcggctggca ggcgcttctg cccaacgacg
    12241 gcctgcgctt ttatcgtccg ctcggtttcc tgctcacctg gcgcatcaac tggccgctgt
    12301 tcggcctcaa ccccttcccc taccaccttg aggggctgct tgtccacgcc gctgccgcct
    12361 gggtggtggg gctgtggctg gccgacatca ccggcaagcg ctggctgggc tggctggcgg
    12421 gggtgctgtt cggggtcttc ccgctgaaca tggaggcggt tggctggctg gctacgcagt
    12481 gggacctgtg ggccgccctg ttcggcactt tgtccgtctg gcagttcagc aagtggtgga
    12541 ggggcgacgg gcgcgacagg cgcgacaggc gcgacgggcg cggcaggcgg ggcctctaca
    12601 tcggttcggt gctcttctac ttcctcggca tcttcaccaa agagagcctg ttcgccttca
    12661 ttcccatcat cgccctctcg gcctggtacg tgctgcctcc caactcgcca ggaaagtggg
    12721 ggcggctagg gctggcgctt gtcccacata cggccctgtt ggggctgaac ttcctcatca
    12781 ggcagatcgc ctggggagag cagcgcaact acacgggcgt ggacatcaac tacttgaact
    12841 tcctgtacga ctggccggtg gaccgcatgc ggttcttgct cgcaccgatt gagagcatca
    12901 tcttcgggga gcctgccgtg caggtggtgg cggcgctctc ggccttgctc ctgctggtgg
    12961 gcctcgtgct gcacgggcgc gatttggccc gcctgctgct cctggccggt gcctggatgg
    13021 cgctgacgct cgtgcccgtg ctcaacatcg gcatcttccg ccacgacttc cagaacagcc
    13081 gactgctcta cctggtgagc gcgggctact gcatcgtggt ggcagcgctg ctgtggagca
    13141 ccatctccca ggcaaaacgg gccaggtggc cgctcatctc gggcacgggc atgctgattg
    13201 tggcatgcgt ggtcgtgacc tggatacaac ttcacccctg gcacacggcc tcggtgcagg
    13261 caaccgaggt ggagagcgag cttgcgcgcc tcatcccgat gcaggagcgg cctcagggga
    13321 tggtctggta tttgaacaac gtgcccgacc ggtacaaggg cgcgtgggtg ctaaactcgg
    13381 ctatgtcgcg cacgcgctac ttgcccggcg gcgcgcccga catccccgat gtggtgaggg
    13441 tagacgaggt ggagaacgtg cccctcggtt cggatgggcg cgactcgttc gctctgcggt
    13501 gggagtttga cccagccggg ccgcgctggc acatccacta cggcctgggc atcacgcgcg
    13561 acagcgaccc ccccgacctc gacaacaata ccggctctaa cctgctgctg tgggacttcc
    13621 ggggctgcga tgcggcggtg ctcatgcagt gggaggtaat caacgcctcc gcaagctgcg
    13681 cgcccggcaa ggggctgctg ctcaagcccg ccaacgaaga cccacaggtc
//
LOCUS       DASRWZ010000428         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_379709, whole genome shotgun sequence.
ACCESSION   DASRWZ010000428 DASRWZ010000000
VERSION     DASRWZ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379709"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFH60_06695"
     CDS             <1..616
                     /locus_tag="VFH60_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6313506.1"
                     /translation="SVASCREFVARGVREFGQIDIACCHAGITSWGKFLEFTEEAFEA
                     VVSVNLRGTYFLAQAAAREMVRRGAGGRIIITSSVTGVQAVENLSAYAITKGGLQMLA
                     RNLVWELSPYNITVNCVAPGSITNERNLRDDPEYATKWATVIPLGRAGEPADVASAVL
                     FFASDEASWITGQTLVVDGGWTAISPTPVFDFVEREGRIGTGEE"
     gene            840..1907
                     /locus_tag="VFH60_06700"
     CDS             840..1907
                     /locus_tag="VFH60_06700"
                     /inference="COORDINATES: protein motif:HMM:NF025839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HET6313507.1"
                     /translation="MPDLFGSEYSPQELRQLVGNMRQVAGIRLLELADGKPRGMRVAE
                     VYTGSGFRFQVFIDRAMDIGYAEHSGRPLAWVHPALGGPQHYEPQGYGWGRTWGGGLV
                     TTCGLTFFGHPEVDAGETLGIHGRIAHIPAEKVRVTEEWRGDDYVLEIEGQVRQVVLG
                     GENLLLLRKISTRLGANSLTIEDTVLNDGLRPSPHMLLYHCNFGYPVVSPQSEIVAND
                     ESVRPRDARAEMGFETVYRFEAPAGPDYDEQVFFHKPHVDGTGHSQTAIINNALNFGG
                     YVRYRAAELPCFAHWKQLSSGEYVCALEPATYWETPRHKLREEDRLKFLQPGERVNYH
                     LELGALDGTEAISDFKQQLLG"
     gene            1927..3381
                     /locus_tag="VFH60_06705"
     CDS             1927..3381
                     /locus_tag="VFH60_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6313508.1"
                     /translation="MHRAALIRELISIIGPEGVISEPEQLVTYESDGLRNHRVLPMLV
                     VLPVSTEQVQAVMRLCHAEGIPVVPRGSGTGLSAGALPVSDGIVLGLSRMREILEVDL
                     DNARARVQPGVLNLWITQRVAPDGYYFAPDPSSQQVCSIGGNVAENSGGAHCLKYGFT
                     VNHVLGLTVVLADGQVVKLGGKGVDKPGYDLVGVLVGSEGTLCVVTEVTVRLVRKPEA
                     VSTLQAAFASPDAAGTAVSDIIGAGIVPAAIEMMDNLSIQAAEAAVHANYPLDAGAIL
                     LVELDGPSVEVAEQMALVLAISERNAATAIKLAANEDERVLMWKGRKSAFAAMGRISR
                     DYYVQDGVIPRTALPTVLREIGALAERYGLRVANVFHAGDGNLHPLVLFDSTQEGETE
                     RAEALAGDILHVCVDAGGSITGEHGVGLDKKRHMPAMFAPADLDTMQLLRCAIDPTRT
                     LNPDKIFPTPRLCGERPGLYKPHPLQEAGLADLF"
BASE COUNT          643 a         1044 c         1182 g          606 t
ORIGIN      
        1 gtcggtggcg agctgccgcg agttcgtggc gcgcggcgtc cgcgagttcg ggcagattga
       61 catcgcctgt tgccacgccg gtatcacaag ctggggcaag ttcctcgaat tcaccgagga
      121 ggcgttcgag gcggtggtga gcgtcaacct gcggggcacc tacttcctgg cccaggcggc
      181 ggcgcgggag atggtccggc gtggcgcggg cgggcgcatc atcatcactt cgtcggtcac
      241 gggcgtgcag gcggtagaga acttgtcggc ctatgcaatt accaagggcg gtttgcagat
      301 gctggcccgc aacctggtgt gggagctatc gccctacaac attaccgtca actgcgtcgc
      361 tccaggctcc atcaccaacg aacgcaacct gcgcgacgac cccgagtacg ctaccaagtg
      421 ggccaccgtg atacccctgg gcagggcggg cgaacctgcc gacgtcgcta gcgccgtgct
      481 cttcttcgcc tccgatgaag cctcctggat cacagggcag accctcgtgg tggacggtgg
      541 ctggaccgct atcagcccga cccccgtgtt cgattttgtg gagcgggagg ggcggattgg
      601 cacgggggag gagtgagttg ctagttgtta gtgtcggata gcggtgagag tctcacgaga
      661 tgaccctgtg ggggtgccct gtggctgaag ccacgggctg accagaggca aagtccccta
      721 cggggactaa ggaaagtgag ggtagtctcc gaaggagact ttgtccctgg tcagcccgcc
      781 aattttttgg cagggcgaag gccacagggc tataccaggg cgtcatgcca ggaggtcgta
      841 tgccggacct gtttgggtct gaatattcac cccaggagct gcggcagttg gtcggcaaca
      901 tgcggcaggt agcgggtata cggttgctgg aactggcaga cggcaagccg cgtggcatga
      961 gggtggccga agtgtacacg ggcagcggct ttcgcttcca ggtgttcatc gaccgggcta
     1021 tggacatcgg ctatgcggag cactcaggca ggccgcttgc ctgggtgcac ccggcgctgg
     1081 gtgggcctca gcactacgag ccgcagggct acggctgggg gcgcacctgg ggcggcggcc
     1141 tggtgaccac ctgcggcctc accttcttcg gccaccccga agtggacgcg ggcgagacgc
     1201 tcggcatcca cggccgtatc gcccatattc ccgccgagaa ggtgcgcgta accgaggagt
     1261 ggcgaggcga tgactacgtg ctcgaaatcg aaggtcaggt gcgccaggtg gtgctcggtg
     1321 gcgagaacct gctcctgcta cgcaagattt cgaccaggct gggggcgaac tcgctcacga
     1381 ttgaggatac ggtgctgaac gacggcctgc ggccctcgcc gcacatgctc ctctaccact
     1441 gcaacttcgg ctacccggtt gtcagcccgc agtccgagat agtcgctaac gacgagtcgg
     1501 tgaggccgcg cgacgcccgt gcggagatgg gcttcgagac ggtctaccgc tttgaagccc
     1561 ccgccgggcc agactacgac gagcaggtct tcttccacaa gccccacgta gatggtacgg
     1621 gccattctca gaccgccatc atcaacaatg cgctaaactt tggtggctac gtgaggtacc
     1681 gcgccgccga gctaccctgc ttcgcccact ggaagcagct ttcttcaggc gagtacgtct
     1741 gcgcgctcga acccgctacc tactgggaga cgcccaggca caagctgcgc gaggaagacc
     1801 gcctgaagtt cctccaaccc ggcgaacggg tcaactacca cctcgaattg ggcgcgctcg
     1861 acggcacaga agctatcagt gacttcaagc aacaactcct gggctaactt aggactggaa
     1921 cccatgatgc atagagccgc acttatacgg gagctaataa gtattatcgg gcctgaaggg
     1981 gtgatttcgg agccggagca gttggtgact tacgagtcgg acgggctgcg caaccaccgc
     2041 gtgctgccga tgctggtggt gctgccggta agcaccgagc aggtgcaggc ggtgatgcgg
     2101 ctgtgccatg ctgaaggtat ccccgtcgtg ccgcgagggt cgggcacggg gctgtcggcg
     2161 ggggcgctgc cggtgagcga cggtatcgtg ctggggctga gccggatgcg ggagatactg
     2221 gaggtggacc tggacaacgc gagagcgcgc gtgcagccag gggtgctgaa cctctggatc
     2281 acccagcggg tcgcccccga cggctactat tttgctcccg acccctccag ccagcaggta
     2341 tgctccatcg gcggcaacgt ggcggagaac tccggcgggg cgcactgtct caagtacggc
     2401 ttcacggtga accacgtcct gggcctgacc gtcgtgctgg ccgacgggca ggtggtgaaa
     2461 ctggggggca agggggtgga caagccgggg tacgacctgg tgggcgtgct ggtcggctcg
     2521 gagggcacgc tctgcgtggt gacggaggtc acggtgcggc tcgttcgcaa gccggaagcg
     2581 gtatcgacct tgcaggcggc gttcgcaagt cccgatgccg cgggcacggc cgtctcggac
     2641 atcatagggg ccgggattgt gcccgccgcg attgagatga tggacaacct gagcatccag
     2701 gccgcggagg ccgccgttca cgccaactac ccgctcgatg cgggggcaat cctgctggtc
     2761 gagctggacg gccctagcgt ggaagtggcg gagcagatgg cgctcgtgct ggctatttcc
     2821 gaaaggaacg ccgcgaccgc gatcaagctg gcggcgaacg aggacgagcg ggtgctcatg
     2881 tggaaggggc gcaagtcggc ctttgccgcg atgggccgca tcagccgcga ctactacgtg
     2941 caggacggcg taataccgcg taccgccctc cctaccgtgc tgcgcgagat tggcgcgctt
     3001 gcggaacggt acggcctgcg cgtggccaac gtcttccatg cgggagacgg caacctgcac
     3061 cctctcgtgc tgttcgactc cacgcaagag ggagagaccg agcgggcgga agcattggcg
     3121 ggcgacatac tgcacgtgtg cgtcgatgcc gggggcagca tcacgggcga gcacggcgtg
     3181 ggcctcgaca agaaacgcca catgcccgcc atgttcgccc ccgccgacct cgatacgatg
     3241 caactgctcc ggtgcgcgat cgacccgacg cgcaccctca acccggacaa gattttcccg
     3301 acgccgaggc tatgcggcga acggcccggc ctctacaagc cgcaccccct ccaggaagcg
     3361 ggattggccg atttgtttta gcttagacat atagcatatt aactaattga ctaattacgt
     3421 gagacatctg caacattttg cctctcatga gcacaaattc agacacccct acaaa
//
LOCUS       DASRWZ010000429         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_310591, whole genome shotgun sequence.
ACCESSION   DASRWZ010000429 DASRWZ010000000
VERSION     DASRWZ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310591"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2245)
                     /locus_tag="VFH60_06710"
     CDS             complement(<1..2245)
                     /locus_tag="VFH60_06710"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6313509.1"
                     /translation="MAIGNMVGASVRRHEDPKLVAGAGNYVDDIKLPGMLHIGIVRSP
                     YAHARITNIDMQALDEHKWIVSKLTGKEIRERQKVPLPITWVIPPDIKKPEHWAMATD
                     IVRYAGEPVAVVAVEDRYRIEDAVEMLSVDYEPLPVVTGIEDALKEGAPILHEELGTN
                     HAYTFSFSTGDMEAAFRNAEVVVKQRMVNQRLAPVSIETRGIVAQYNKPMNELTVWSS
                     TQTPHLTKHHLSEMLGIPEHNVRVIAPDVGGGFGSKLNILPEDFIVPLMAIVTGRPVK
                     WIETRRENLQNTVHGRDMVDYIEIAARRDGTIMGVKATIYANLGAYCRFFAAGLPILA
                     GLMLPGNYKLPAFQVDIHTAYTNTLFVDAYRGAGRPEATYAIERIMDMLADELGMDPV
                     ELRRKNLVQEEDWPYTSPTGLAYDSGNYVPTLDKALEMAGYDEARTRQQEHNSQNPRV
                     RKGIGLAAYVEIAGWGPGQATKGLGVANQLFGSATVRVDRSGTVEVLAGASGHGQGHT
                     TSWAQIAADQLGIGHEQVRVYEGDTAMIQTGTGTFASRSLSVDGAGVHLAAQKVRDKM
                     LKIAAHQLEVDPADLEMRDGMITVRGVPLEEEVVAEGKLQTEESAAEPGHPRAVSFKD
                     IAISANTGHDLPADMEPGLTETAFFDPVNFTYPFGFHVAEVDVDTETGEVKITRYIAV
                     DDCGNVINPMIVDGQVHGGVAKGVAQALWEEVVYDENGQLVTGTLMDYAVPFSSDLPM
                     FETAATIT"
     gene            complement(2349..>2770)
                     /locus_tag="VFH60_06715"
     CDS             complement(2349..>2770)
                     /locus_tag="VFH60_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6313510.1"
                     /translation="GTHIGCETSQCGACTVHLDGQAVKSCTIFAVQADGSNVTTIEGL
                     SHAASGNGHGDMHVLQTAFWEQHGLQCGYCTPGMIMAAATLLKNNPHPSEDEIRHGLE
                     GNVCRCTGYHNIIKAVQAAAGEDVSERSSRGEAMPTK"
BASE COUNT          439 a          848 c          855 g          628 t
ORIGIN      
        1 gtgtgatagt cgcggcggtc tcgaacatgg gcaggtcgct gctgaagggc accgcgtagt
       61 ccatgagggt gcctgtgact agctgtccgt tctcgtcgta gaccacttcc tcccatagcg
      121 cctgggccac accctttgcc acgccgccat gcacctgccc atccactatc atcgggttga
      181 tgacgttgcc gcagtcgtcc accgctatat accgcgtgat cttcacttcg cccgtctcgg
      241 tatctacgtc cacttccgcg acgtggaagc cgaacggata ggtgaagttg acggggtcga
      301 agaaggcggt ctcggtgagg ccgggttcca tgtctgccgg taggtcgtgg ccggtgttag
      361 cggatatagc tatgtccttg aacgatactg ctcttgggtg gcctggctcg gcggcggact
      421 cctcggtctg caacttaccc tcggcaacga cctcttcttc caggggcacg ccgcgcaccg
      481 taatcatgcc gtcgcgcatc tcaaggtcgg cggggtccac ctcaagctgg tgggcggcta
      541 tcttgagcat cttgtcgcgc accttctggg ccgccaggtg tacgcccgcg ccgtccaccg
      601 acaggctgcg gctggcgaac gtaccggtgc ccgtctggat catggcggta tcgccctcgt
      661 agacacgcac ctgctcatgc ccgatgccca actggtcggc agctatttgc gcccagcttg
      721 tcgtgtgacc ctggccgtgc ccgctcgctc ccgcaagtac ctcgacggtg ccggagcggt
      781 ccacccgcac ggtggcgctg ccaaatagct ggttggcgac gcccaggccc tttgtcgcct
      841 gacccggacc ccaccccgct atctccacgt aagcggcaag ccctataccc ttgcggacgc
      901 gcgggttctg gctgttatgc tcttgctgtc gggtgcgggc ctcgtcgtaa cccgccatct
      961 ccagcgcctt atcgagcgtg ggcacgtaat tgccgctatc gtaggcaagg ccggtggggc
     1021 tggtgtaggg ccagtcctcc tcctgcacaa ggttcttgcg gcgtagctcg acggggtcca
     1081 tgcccagttc gtcggccagc atgtccatga tgcgctcgat ggcgtaggtg gcttccggcc
     1141 tgcccgcgcc cctgtaggcg tcaacgaaga gggtattggt ataggcagtg tggatgtcca
     1201 cctggaatgc ggggagcttg tagttgccgg ggagcatcag gcccgccagg atagggaggc
     1261 ccgccgcgaa gaagcggcag tacgcgccca ggttggcgta gatcgtggcc ttcacgccca
     1321 tgatggtgcc gtcgcgccgc gcggctatct ctatgtagtc caccatgtcg cgcccatgca
     1381 cggtgttttg caggttctcc cgccgcgtct ctatccactt gaccgggcgg cccgtaacta
     1441 tcgccattag gggcacgatg aaatcttcgg gcaggatatt gagcttcgaa ccgaacccgc
     1501 cgcccacgtc aggggctata acgcgcacgt tatgctcagg gatgccgagc atctcggaga
     1561 ggtggtgctt ggtcaggtgg ggagtttgcg tgctcgacca gacggtaagc tcgttcatcg
     1621 gcttgttgta ctgagccacg ataccgcgcg tctcgatgga gactggggca agacgctggt
     1681 tgaccatgcg ctgcttcacg accacttcgg catttcgaaa ggcagcctcc atgtcgcccg
     1741 tgctaaagga gaaggtgtag gcgtggttgg tgccaagctc ctcatgcagt ataggcgcgc
     1801 cttctttcag cgcatcctca atgccggtca cgacgggcag aggttcgtag tccaccgaaa
     1861 gcatttcgac cgcgtcctcg atgcgatagc ggtcctctac agccacgacc gctaccggct
     1921 ccccggcgta gcgcactata tcggtagcca tcgcccaatg ctcaggcttc tttatgtcgg
     1981 gcgggatgac ccaggtgata ggtagaggca ccttctgccg ctcgcgtatc tccttgccag
     2041 tcagcttgct cactatccat ttgtgctcgt ccaatgcctg catatctatg ttggttatgc
     2101 gggcgtgggc gtaggggctg cgcacgatgc cgatatggag catccccggc agctttatgt
     2161 cgtctacgta attgcccgcc cccgccacca gtttcgggtc ttcgtgcctg cgaacgctgg
     2221 caccgaccat attgccgatt gccatgtcta gctctccttt aggtacgatt tggaatttcg
     2281 aatttcggat ttcgaaaaga cgttcgctgt cggctcaatt tcgcaattcg caattccaaa
     2341 tttcgaaatt acttggttgg cattgcttcc ccacgcgacg acctctcgct cacgtcttct
     2401 ccggctgcgg cctggaccgc cttgattatg ttgtggtagc cggtgcagcg gcagacgttg
     2461 ccttccaggc cgtgacgtat ctcgtcttcg ctggggtgag ggttgttctt gaggagggtg
     2521 gcggcggcca tgatcatgcc tggggtgcag tagccgcatt ggaggccgtg ttgttcccag
     2581 aaggctgttt ggaggacgtg catgtcgccg tgcccgttgc ctgacgcggc atggctgagg
     2641 ccttcgatgg tggtgacgtt cgagccgtcg gcctgcaccg cgaagatggt gcatgatttc
     2701 acggcctgcc catcgaggtg gaccgtgcat gccccgcatt ggctggtctc gcagccgatg
     2761 tgcgtgccgg
//
LOCUS       DASRWZ010000430         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_379782, whole genome shotgun sequence.
ACCESSION   DASRWZ010000430 DASRWZ010000000
VERSION     DASRWZ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379782"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..987)
                     /locus_tag="VFH60_06720"
     CDS             complement(268..987)
                     /locus_tag="VFH60_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6313511.1"
                     /translation="MDTPNTRGSNEQDSRDSRTSEGPTPKPVEGRDMVGEGRSLAPEQ
                     DPVNAVNPMASPSSYSLNAVEAGDTPLDEELDSLVDPDIGREQLSNNPDVLNLDPSWR
                     VESEEPDFMSDPGTTDIIEAIEEGEPYFPPTDPPLRTVGLNNVETLGGFSITSLEEPT
                     EPEDDPVRLQLNDDEIAGRVRYALATDAYTADLNIEVEVEDGVVFLHGSVGSLDDIEQ
                     AEQIAGSVPGVVEVEEDLEIV"
     gene            1293..>2755
                     /locus_tag="VFH60_06725"
     CDS             1293..>2755
                     /locus_tag="VFH60_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6313512.1"
                     /translation="MADNTGGENTGGVVGRVLNGRYQLISVVGGGGMAQVYKSRDNIL
                     GRIVAIKVLREQYSTDAQFVARFRREAQAAANLAHPNIVNVYDVGQDGDTHYIVMEYI
                     NGESLKSYINRSSPLPIGKALSVAAQILAGLEYAHRSGLIHRDIKPQNVLITPDGAVK
                     VTDFGIAKSVSDLGLTEAGLALGTAHYFSPEQAKGERVVPQSDIYAVGVTLYEMLTGR
                     LPFESENVMGLAYKHISEEPPSARNLNPSVPPRLDATILKSMAKDPQQRFGSAAEMEK
                     ALKAIQQGGQQPTMEVPVAQVNVQPRQRGQQPPQVGARTGRMGASGPSTGSMRVGAGP
                     RGAYAMGGAAAGALPARGMTSPLGSPTSMAVSPVPRAQSGSGSSGCLIAAITFITVGI
                     LAVLVGGVIFFGPRLPGFFQDVVVPSPTPTPTVPTATPTNTAVPPTNTPTSTPTLTPT
                     PTSTPISVPVPRLVGLNIQNARTLAQQHGFLVLELDR"
BASE COUNT          519 a          980 c          743 g          513 t
ORIGIN      
        1 gttggagatt agcacctgtg tacgcctgtc acccagggca ggcacggggg cctgccccta
       61 caaatcatta ccttattcag ttgttaatct tcataagcca cgggcttaca aggggcaaag
      121 tcccctacgg ggactaagca acatgagggc agtctccgaa ggagacttcg tccctggtaa
      181 gcccgccaat tcattggcag ggcgccatgc agggcgtccc cgcagggcag cctccatcac
      241 taacaactaa caactaacaa ccaacaacta aacaatctcc aggtcctcct ccacttccac
      301 cacccccggc acgctacccg ctatctgctc cgcctgctct atgtcgtcca gcgaccccac
      361 cgaaccgtgc agaaacacca ccccgtcctc cacctccacc tcgatgttga ggtccgccgt
      421 gtaggcatcg gtggcgagag cgtagcgcac ccgcccggct atctcgtcgt cgttgagttg
      481 caggcgcacc gggtcgtcct ccggctcggt cggctcctcc agcgaggtaa tcgagaagcc
      541 ccccagcgtc tccacgttgt tcagcccaac cgtccttaac ggcgggtcgg tcggcggaaa
      601 atacggctcc ccttcctcta tcgcttcgat aatatcggta gtgccggggt ccgacatgaa
      661 atcaggctct tcgctctcca cccgccagct tgggtccagg ttcagcacgt cggggttgtt
      721 gctcaactgc tcgcgcccaa tatccgggtc caccagcgaa tcaagctcct cgtcgagagg
      781 cgtgtccccc gcctccaccg cgttcaacga ataactgctg gggcttgcca tcgggttgac
      841 ggcgttcacc gggtcctgct ccggtgccag gctccgcccc tcgcccacca tatcccggcc
      901 ctcgaccggc tttggggtcg gcccctccga cgtcctgcta tcccgcgaat cctgctcgtt
      961 agagcctcgc gtgttcggtg tgtccatcag tccctccgtg cgtagcccat gatgggccta
     1021 ttgtagcata cggatttgga atttcgaatt tcgaatttcg aatttggtag atatctaggc
     1081 aaatccgaaa ttcgcaattc gaaattccaa atctcttctc cgtttgacag gctatatagg
     1141 ctggtttaca atctttatca ggagtacagt gcgtacgagc gtatgtgcaa aagtgtgcgc
     1201 cctgctccgt gctgggtccg agactatagc gcaaggtgtg tgccagcggg cagttgtgcg
     1261 tcgatgcgtc gtcatgggaa tcggggtaga tattggctga taacaccggc ggggaaaata
     1321 cgggcggcgt cgtaggtcgc gtgctcaacg ggcggtacca gctcatcagc gtggtgggcg
     1381 gcggtggcat ggcccaggtc tacaagtccc gcgacaacat cctgggccgc atcgtcgcga
     1441 taaaggtgct gcgcgagcaa tattccaccg acgcgcagtt cgtggcccgc ttccggcgcg
     1501 aagcccaggc cgccgccaac ctcgcccacc ccaacatcgt caacgtctac gacgtgggcc
     1561 aggacggcga tactcattac atcgtaatgg agtacatcaa cggcgagagc ctcaaaagct
     1621 acatcaaccg ctcctcgccc ctgcccatcg gcaaagccct ttcggtggcc gcgcaaatac
     1681 tcgcgggcct cgaatacgcc caccgaagcg gcctcatcca ccgtgacatc aagccgcaga
     1741 acgtgctcat caccccggat ggcgcggtca aagtcaccga cttcggcatc gccaagagcg
     1801 tgtccgacct cggcctcacc gaggcgggcc tggcgctggg caccgcccat tatttctcgc
     1861 ccgaacaggc caagggcgag cgcgtggtgc cccagtcgga tatctacgcg gtcggcgtga
     1921 ccctctacga aatgctcacc gggcgcttgc cgttcgagag cgaaaacgtc atgggcctcg
     1981 cttacaagca catctccgaa gaaccgccct ccgctcgcaa cctcaaccct tctgtgcctc
     2041 cccgtctcga cgccaccatc ctcaaatcga tggctaaaga cccgcagcag cgtttcggca
     2101 gcgcggcgga gatggagaag gccctcaagg cgatacagca gggcggccag caacctacca
     2161 tggaagtgcc cgtcgcccag gtgaacgtgc agccccggca gcgcggccag cagccgcccc
     2221 aggtaggtgc caggaccggg cgcatgggcg catctggacc ctctaccggc tctatgcgcg
     2281 tcggcgcggg gccgcgaggg gcctatgcta tgggtggcgc cgccgcgggc gctctccctg
     2341 ccaggggcat gaccagcccc ctcggcagcc ccacctcgat ggccgtctcc cccgtgcctc
     2401 gcgcccagtc cggctccggt agctccggct gcctgatagc cgctatcacc ttcattaccg
     2461 taggcatact ggctgttcta gtgggcggtg ttatcttctt cggcccgcga ctccccggct
     2521 tcttccagga cgtggtcgta cccagtccca cgcccacccc caccgtgccc accgccaccc
     2581 ccaccaacac cgccgtgccc cccaccaaca cccctacctc caccccgacc ctcaccccca
     2641 cgcccaccag cacgcccatc tccgtgcccg tacccaggct ggtcggcctg aacatacaga
     2701 acgccaggac cctagcccaa caacacggtt tcctcgtgct ggagctagac cgcat
//
LOCUS       DASRWZ010000431         4819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_790359, whole genome shotgun sequence.
ACCESSION   DASRWZ010000431 DASRWZ010000000
VERSION     DASRWZ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790359"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..707
                     /locus_tag="VFH60_06730"
     CDS             192..707
                     /locus_tag="VFH60_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET6313513.1"
                     /translation="MPGHDIIVIGASAGGVEALTSLVKRLPEDLAAAVFAVLHISPEL
                     PSRLAEILSRAGKLPATQPRHGEEIRPGHIYVAVPDRHLLVHRGHISVVHGPKENRYR
                     PAIDTLFRSAARSYGPRVVGVVLTGAQARYFTYTAVPLHATNDNVDGVVIYGDEVPGP
                     AADASHNGASG"
     gene            1178..2878
                     /locus_tag="VFH60_06735"
     CDS             1178..2878
                     /locus_tag="VFH60_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET6313514.1"
                     /translation="MDFSNYGRESTFKHYKVLLVNDNPEEVAVIMRLLGRSLHARFEV
                     THVDWMREALHALQASRFDVVLADLFLPDRSGVEIVTEMCSITPSVPIVAMARIGTER
                     MAAEALRAGADDYFQLGPDTSGESNLLLRVVHHVIERKQLTDRLNFLHEATDTLSASL
                     DLQDTVSAIGRVVVPRVADCCILEFLDGSGKTTRAAVCGFRPKLDTDQPGEGVQMDAV
                     TDDNREQHLAWSPVVPRLMSAGKAELFAEMDDQVLQTLSYSPGHFQKLHALDIKSMIV
                     APLTSGGSSLGSLSFITLDGRPGYGEADLSLVEEFSRRCAVALDNALLYERVEKALES
                     QEELLARVSHDLRTPLTSIRAGLGLLTMNIGSKLSPGEASLLDNVKRGAERLGLLVND
                     LLAQNEISQDAPLEKEPVDLRALVEDVVLLLRPLVQGKDQQMRVDLPGPLPYFANGKL
                     LGQAFTNLLDNAHMHTPSGTTITISGRVTPTEVVLVISDDGPGIPPELHESIFQRFYK
                     APSARRGRGLGLGLTIARSVVEMHGGSLHVESTPGEGTSFYISLPSPAGEADEAVDTP
                     "
     gene            2909..3637
                     /locus_tag="VFH60_06740"
     CDS             2909..3637
                     /locus_tag="VFH60_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6313515.1"
                     /translation="MTITILIAEDEQDVAELVSYGVRMNWPDCEVLLAEDGTEALRLI
                     QAERPNLVVLDIGMPAPDGFEVLKAIRETSQVPVLMLTARNATMDKVKALELGADDYL
                     TKPFDHLELLARLRALVRRATITGALSQTEEAAPTPADAQRVEIGDLSVNYATREVCL
                     RGKVLALTSTEYRLLEELVRHAGEVLSHRHLLERVWGPEYIGEDHYLKVFVRRLRSKL
                     GDDADRPRYIQTEWSIGYRFIPAG"
     gene            3756..>4819
                     /locus_tag="VFH60_06745"
     CDS             3756..>4819
                     /locus_tag="VFH60_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313516.1"
                     /translation="MNGLAYGKRRALNEMRSIFGCQWASGMLPQIRFMAGPEGGGEAG
                     YRPNADDWGVTSEISGPTRLRTSGITQPPIVGLCAHDLFRKLTEAERAAHLADFLAFS
                     HGLRRFHTWLLSERDPWGEGLALCLHPWETGTDNSPAFDPLIEATRLYAQATNLPVHL
                     FGRADTTHVPHWQRPTDRDYFSYFGLVALFKKLHYRQGDIIEASPFLLQDVLFNSLLA
                     ASLLAHAHLQVEIAELLSGGHSKGADRGELLAGAEESRELYNRVSAAIRRKLWHPDDG
                     FFYSFDSRENRPLPMPTISGFGPLLPGIASNEQAARLVEHLTLPGRFGTPVPVPSTPA
                     DSPAFDPLIEATRLYAQATNL"
BASE COUNT          931 a         1620 c         1445 g          823 t
ORIGIN      
        1 tcgctacgct caggatgaca ggggccacct ttggtcccac tgccccaccc cgacgctaac
       61 ccagtcgctc acgcgccact ccccaacgct ggcatacctt ctgcttccac ttcttgtgtg
      121 cgcttcgtcg caacctctca cccacggtga caaatatcac ccgacaccaa atatgcgact
      181 cggaggtata gttgcccgga cacgatatta ttgtaattgg agcctccgcc ggtggcgtgg
      241 aagccctcac ctccctggtg aagcgtctcc cggaggactt ggccgccgcc gtcttcgcgg
      301 tgttgcacat ctcgcccgaa ctcccaagca ggctggcgga gatcctcagc cgcgcgggca
      361 agctcccggc cacccaacca cggcatggtg aagagattag gccggggcac atctacgtgg
      421 ccgtccccga ccgccatctc ctggtgcaca ggggacatat cagcgtcgtg cacggcccga
      481 aagagaaccg gtacaggccg gcgatagaca cccttttccg ctcggcggcc cgctcgtacg
      541 gcccgcgcgt ggtgggcgtg gtgctcaccg gcgcgcaagc ccggtacttc acctatacgg
      601 ctgtccccct ccacgccaca aacgacaacg tggacggcgt ggtgatatac ggcgacgaag
      661 tacccggacc tgcggcggac gcctcccaca acggggcgag cggctaagaa gcgctctccc
      721 agactgccgc cctacgccct ccacgcgtcc gctcgctgtt accctggcac gcattgtgtt
      781 tagccaagta ccttagcttc ctcagttcac atgcgacgca agccccgcag tcagcctgag
      841 cttcccccca ctgtcatcct tagctacgct caggatcagg tgggcaggcg agagtctaca
      901 atcttgagga tgatggtggc tagaggaaca atggtgctca agtaggaaga atcaaacacg
      961 ggcaactgat ccttcgctac gctcaggatg acagagagtc gctaaggctg acaggggagc
     1021 ggctcgggat gacagagagt cgcttggggg ttcgctagca agccggacgc cggtatgaat
     1081 acgctacacc aaccacgcag ccgaggctgg tagcctcaac gggcgcacct gcactgccaa
     1141 tcgccgcgca tctgcaaacg ccccattgtg cggggacatg gacttcagta actacggccg
     1201 agaatctaca ttcaagcact acaaagtgct gctcgtcaat gataacccgg aagaagtagc
     1261 cgttataatg cggctcctgg gcaggtccct ccatgcccgc ttcgaggtga cgcacgttga
     1321 ctggatgagg gaggccctcc acgccctgca agcgagccgc ttcgacgtcg tgctcgccga
     1381 cctctttctg cccgacaggt cgggcgtcga gatcgttaca gagatgtgct cgattacgcc
     1441 ctcggtgccc atcgtagcta tggcaaggat cggcaccgag aggatggcgg cggaggccct
     1501 ccgcgccggg gccgacgatt acttccagct aggccccgac accagcggcg agagcaactt
     1561 gctgctccgc gtggtacatc acgtaatcga gcgcaagcag ctaaccgaca ggctgaactt
     1621 cctgcacgaa gcgaccgaca ccctctctgc gtcgttggac ctgcaagaca cggtcagcgc
     1681 gattggtaga gtggtggtac cgcgcgtcgc tgattgctgc atactggagt tcctggacgg
     1741 ttccggcaag actacgcggg cggcagtatg cggcttccga cccaaacttg acaccgacca
     1801 gcccggtgaa ggcgtccaga tggacgccgt caccgacgat aaccgggagc agcatctcgc
     1861 ctggtctccc gttgtgcctc ggctcatgag cgcgggcaaa gccgaactgt tcgcggagat
     1921 ggacgaccag gtgttgcaaa cgttgtccta ttcgcccggc cacttccaaa agctgcacgc
     1981 gcttgacatc aagtcgatga tagtagcgcc cctcacctcg ggcggaagct cgctgggcag
     2041 cctttcgttt ataacgctgg atgggcggcc tggctacgga gaggcagacc tctcgctggt
     2101 ggaggagttt tctcgcaggt gcgccgtggc cctggacaac gccctcctct atgagagagt
     2161 ggagaaggcc ctggaatcgc aggaggagtt gctggcccgt gtgtcccacg acctgcgcac
     2221 gccacttacc tcgatacgcg ccgggctggg cctgctgacg atgaacatag gctcgaagct
     2281 gtctcccggt gaggcctcgt tgttggacaa cgtcaagcga ggagccgagc gcctggggct
     2341 actggtgaac gacctgctgg cccagaatga gatcagccag gacgccccgc tggagaagga
     2401 gcctgtcgat cttcgcgccc tggtcgagga cgtggtcttg ctgctacggc ccctggtgca
     2461 ggggaaggac cagcaaatgc gagtggacct gccagggccg ctgccttact tcgccaacgg
     2521 caagctgctg gggcaggcct tcaccaactt gctggacaac gcccacatgc acactccatc
     2581 aggcactaca atcaccatat cgggccgcgt cacacctacc gaagtggtgc tggtaataag
     2641 cgacgacggc ccaggcatcc ccccggagct gcacgagtcc atattccagc gcttctacaa
     2701 ggccccctcg gcaaggcgcg gcaggggcct ggggctgggc ctgacaatcg cgcgctccgt
     2761 cgtggagatg cacggcggca gcctgcacgt cgagagcacg ccgggcgaag gcaccagctt
     2821 ttacatcagc ctgcccagcc cggccggtga ggcagatgag gcggtagata ctccatagag
     2881 gcaaattttg aagagggggg gactcctgat gacaatcacc atattgatag ccgaggacga
     2941 gcaggacgta gccgagttgg tgtcgtatgg cgtgcgcatg aactggccgg actgcgaggt
     3001 gctgctggca gaggatggta cagaggcgct ccgcctgatc caggcggagc gccccaatct
     3061 tgtcgtcctc gatattggca tgcccgcgcc tgacggcttc gaggtgctca aggccatcag
     3121 ggagacctcc caggtgccag tgttgatgct caccgcccgc aacgctacga tggacaaggt
     3181 caaggccctc gaactggggg ccgacgacta cctcaccaag cccttcgacc acctggaatt
     3241 gctcgcccgc ctgcgcgccc tcgtccgccg cgccaccatc accggcgcgc tctctcagac
     3301 cgaggaagcc gcccccaccc ccgccgacgc gcaacgagtg gaaattggcg acctgtcggt
     3361 gaactacgcc acgcgggaag tatgcctccg gggcaaggtg ctcgccctca cctcgaccga
     3421 gtatcgcctg ctggaagagc ttgtgcgcca cgccggggag gtgctcagcc acaggcacct
     3481 gttggagcgc gtgtgggggc cggagtacat aggcgaggac cactacctca aggtgttcgt
     3541 gcgccgcctc cgcagcaaac tgggcgacga cgccgaccgc ccccgctaca tccagaccga
     3601 gtggagcatc ggctaccgct tcatcccggc cggatagtgg ttattgatta gcaagagacg
     3661 aaaacgcaaa gacgcgaagg acgcaaagaa cgcgcaaagg tgattaaagg tctggttagc
     3721 tcccactgac cactgaccac aacggagcta cagctttgaa cggccttgcc tacggaaagc
     3781 ggcgcgccct caacgagatg cgttccatct ttggctgcca gtgggcgagc gggatgctgc
     3841 cgcagatacg cttcatggcg gggccggagg gtgggggcga ggcaggctac cgacccaacg
     3901 cggacgactg gggcgtgacg tcggaaattt cggggccaac gcggctgcgc acctccggca
     3961 tcacccagcc ccctattgtt ggcctttgcg cccacgacct ctttcgcaag ctcaccgagg
     4021 ccgagcgcgc cgcccaccta gccgacttcc tggcgttcag ccacggcctg cggcgtttcc
     4081 acacctggct gctctccgag cgcgacccct ggggcgaggg gctggccctc tgcctccacc
     4141 catgggagac gggcaccgac aactcccccg ccttcgaccc cctcatcgag gccacccgcc
     4201 tctacgccca ggcaaccaac ctgcccgtac acctctttgg ccgcgccgat accacgcacg
     4261 tcccacactg gcaacgcccc accgataggg actacttctc ctacttcggc ctggtggccc
     4321 tcttcaagaa gctgcattac cgccaggggg acatcatcga ggcaagccct ttcctgctgc
     4381 aagacgtgct cttcaacagc ctgcttgccg cctccttgct cgcccacgcg cacttgcagg
     4441 tagagatagc cgagttgctg tccggcggcc acagcaaggg ggcggacagg ggggagcttc
     4501 tggccggggc ggaagagtcg cgggagctat ataaccgggt gtcggcggct atacggcgca
     4561 agctgtggca ccctgacgac ggcttcttct acagcttcga cagccgcgag aatcgcccgc
     4621 taccgatgcc caccatctcc ggcttcgggc cgctcctacc cggcatcgct tccaatgagc
     4681 aggcggcgag gctggtcgag catctcaccc tccctggtcg cttcggcacg cccgtgccgg
     4741 tcccctccac ccccgcggac agccccgcct tcgaccccct catcgaggcc acccgcctct
     4801 acgcccaggc aaccaacct
//
LOCUS       DASRWZ010000432         6970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_516249, whole genome shotgun sequence.
ACCESSION   DASRWZ010000432 DASRWZ010000000
VERSION     DASRWZ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516249"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFH60_06750"
     CDS             <1..526
                     /locus_tag="VFH60_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313517.1"
                     /translation="TPEEGIRLSDAVFSGHIVGITMPPPNPVNGVYSPALAEFRTAFV
                     WKGAVGPNVQVSYSPSEASCGYDFHTGADYLVYAHSYVDGPTLSIFGNSFQLPLGGSK
                     LTTGICMRTRLLSTAQHDLAVLGQGASPTASGLLDIVLDNLLAVVAVVAALLIVLVLY
                     LLRRRRRRSFRLKP"
     gene            610..1692
                     /locus_tag="VFH60_06755"
     CDS             610..1692
                     /locus_tag="VFH60_06755"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET6313518.1"
                     /translation="MANTQPAGAGRVAVVGTGSWGTTLAILASRQGRPTHLLARTPEE
                     ASLLASEAENRRFLPGFAFPHGLTTTSEPQEALRDCDLLLMVVPSQTMRANIRALKPY
                     LQAERTIVVSCAKGLELGTMLRMSEVIVQELGEEWSEQVGALSGPNLAKEIVASKPAT
                     TVVASTSLDTARKAQELLTGGRFRVYTNPDLVGVELAGALKNIIALAAGMADGMEAGD
                     STKAALITRGLVEIGRLGLAAGASLFTFAGLAGLGDMIATCASPLSRNRTMGEMLARG
                     KTLEEVKAQFGGQVAEGVTTTVAAREMAVSKGVEMPITEQLYQVLFEGKPPMSGLNAL
                     LTREPTDEFLSLGVNIPDDSEDEGLG"
     gene            complement(1881..2618)
                     /locus_tag="VFH60_06760"
     CDS             complement(1881..2618)
                     /locus_tag="VFH60_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313519.1"
                     /translation="MSLQQTTNQASNLSPNVPSTHNAWLYWVLANAVGHIAGISAFGI
                     VTLFINWGGSGGVAALFDLALPFAVLGLLDGLLVGLAQGLVVQHFTGRPLLGEWALFT
                     TLGGIAAWVLGTLVGGLTIVIIGILFYIAAGGIAGVLMGYAQRPTVQRHLDPQAGWII
                     PNMLAGSLAVTAGIAFSSLWGNPENTGGITGGVEIIILAVGLGGLLYGAVTARSFATM
                     LDFYAARSSYGSDEPVEDVSKLPVHRA"
     gene            complement(2780..4168)
                     /locus_tag="VFH60_06765"
     CDS             complement(2780..4168)
                     /locus_tag="VFH60_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6313520.1"
                     /translation="MLVYVATRAGTPTTAPDIGRNVNPGMSAIRPGAPGDWPMLGRDL
                     SRTNFNPDEQTLTAANIGDLAPRWRAVVNPERIPSGSAPSVANGRLYIGGSADEGPNF
                     FAFDASTGVPLWQINLGDAPEDCADVSIDVGIGSTAAISGSIAVVGGADAAFYGLNAD
                     TGATLWRDPLNVGPSGFAWASPLLAHGRAYYGVASYCDRPSVRGEVRSVDLGSGRLVA
                     NRFIVPEGKSGGGVWHAPSLTPDARTLLVATGEDFEGYDGPLNRALISLDALSLEVKQ
                     SDKQGSLNVDHDWGSTPVIISDSQGRTLAAASHKDGTFRAYNLDSIGGGPIWGREVKS
                     AVAMTPAYDPTLGPGGTLFFVGHKDNGIQLYAVDPATGADRWTPATLPDYPFGSMAIA
                     NGLVFLNLTGVLHVYDEHTGALLRAVEPQDAGPSWTGPSVAHGFVYWTSGAYLNAWSL
                     PGPATAPATPAP"
     gene            complement(4342..5700)
                     /locus_tag="VFH60_06770"
     CDS             complement(4342..5700)
                     /locus_tag="VFH60_06770"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET6313521.1"
                     /translation="MAVVAVIGCQWGDEGKGKIVDLLAEQVGVVARCNGGTNAGHTIV
                     NELGTFRLRLTPSGIFNPQAVCIIGNGVVIDPEVLLDEMDSLTAAGVDVSRLLISDRA
                     HVIMPYHKLLDGLEEDAREGTGTGPIGTTRRGVGPAYMDRTARVGIRMGDLLNDESLL
                     TKLSSVIPAKNALLSQVYNTTPIDVHKTYLRLVEQGQRLRKHIMPTELVITRAITDNR
                     SVLLEGAQAALLDLDFGTYPYVTSSNPTAAGLCVGSGTPPNQLSSIVGVFKAYQTRVG
                     AGPFPTEIQGELATFLREQGGKGHGEYGTVTGRPRRVGWFDAVAGRYVCQFNGLTGLA
                     ITRLDALDQMETIRICTAYKVNDAHLNHMPADIEMLAQVEPQYDEYPGWLTDTSGARK
                     WSDLPDNARHYLDRLTAILQTPIDMVSVGPHREQTIVLRDPFNPVPQQVEKLVQSLDR
                     EG"
     gene            complement(5768..6154)
                     /locus_tag="VFH60_06775"
     CDS             complement(5768..6154)
                     /locus_tag="VFH60_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET6313522.1"
                     /translation="MQAKIGRFEDLVAWQKARVLTRDIYAVTQQGRFAKDFSLSGQIQ
                     RAAVSIMSNLAEGFERGSRAEFHHFLSIAKSSCAELRSQLYVALDIGYIGEDTFRQIM
                     LQAEEVGRIIGGLRSSVEKQRDSQKV"
     gene            complement(6502..6970)
                     /locus_tag="VFH60_06780"
                     /pseudo
     CDS             complement(6502..6970)
                     /locus_tag="VFH60_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
BASE COUNT         1225 a         2118 c         2278 g         1349 t
ORIGIN      
        1 cacccctgaa gagggcatac gtctatctga tgcggttttc agcggccaca tagtgggtat
       61 caccatgccg ccgccaaacc ctgtaaatgg cgtctactcg cccgcactag ccgagttccg
      121 aacggctttc gtgtggaagg gggcggtggg gccgaatgtg caggtgagtt atagcccaag
      181 cgaagcctcg tgcggctacg actttcacac gggggctgac tacctggtat atgcccactc
      241 ctatgtggat ggcccgacgc tctccatctt cggcaactcc tttcagttgc ccctcggggg
      301 gagcaagctg acaacgggca tatgcatgcg cacccggctg ttatctaccg cgcagcatga
      361 cctggcggtg ctcggccagg gcgcttctcc tacagcgagc ggcttgctcg acatagtgct
      421 cgacaacctg ctggccgtgg tggctgtggt ggcggcgctg ctcatagtcc tcgtgttgta
      481 cctgttgagg cgcaggcggc ggcgcagttt caggctgaag ccgtaagcca tagggcgctt
      541 gccctatgct ataatggccc atgctcggcg gctggtaggg gcagattcag gctggaggaa
      601 gagctagaga tggcgaacac acaaccggcg ggcgcagggc gcgtggcggt ggtcggcacg
      661 ggtagctggg gcaccaccct ggcgatactg gcctctcgac agggccgccc gacccacctg
      721 ctggcccgca cccccgaaga ggcctccctc ctcgcctccg aagccgagaa ccgccgcttc
      781 ctgcccggct tcgccttccc tcacggcctt accaccacct ccgaacccca ggaggccctc
      841 cgcgattgcg acctgctgct gatggtggtg ccttcgcaga cgatgcgcgc caacatccgc
      901 gcgctcaagc cctacttgca ggctgagcgg accattgttg taagctgcgc gaaggggctg
      961 gaactgggca ctatgctccg catgagcgaa gtaatcgtgc aggagctagg cgaggagtgg
     1021 tcggagcaag tgggggcgct gtcggggccg aacctggcga aagagatagt ggccagtaag
     1081 cccgcgacca ccgttgtggc ttccaccagc ctggacacgg cacgcaaggc gcaggagctg
     1141 ctcacggggg gccgcttcag ggtctatacc aaccccgacc tggtgggcgt ggagcttgcg
     1201 ggggcgctca agaacatcat cgcgctcgcg gcgggcatgg ccgacggcat ggaggcgggc
     1261 gactcgacga aggcggcgct catcacgcgt gggctggtgg agatagggcg tctggggctg
     1321 gcggccgggg cctccctctt caccttcgcg gggcttgcgg gcctgggcga catgatcgcc
     1381 acctgtgcct cgcccctgtc gcgcaatcgc acgatgggcg agatgctggc gcgcggcaag
     1441 acgttggagg aggtgaaggc ccagttcggg ggccaggtgg ccgagggggt gactaccacg
     1501 gtcgccgcga gggagatggc ggtcagcaag ggtgtcgaga tgcccatcac ggagcagctt
     1561 taccaggtgc tgttcgaggg caagccgccc atgtcgggcc tgaacgccct gctcacgcgc
     1621 gaaccaaccg acgagttcct gtcgctgggg gtgaacattc ccgacgattc ggaggatgaa
     1681 gggttgggtt gattgcctgg gatagtcctg tgggtgtgcc ctgccaatga attggcgggc
     1741 ttacaagagg caaagtcccc tgcggggact aaggaacatc atgtagtctc cgaaggagac
     1801 ttcgtccctt gtcagcccgc caatttattg gcagggcaca cgtcgcgggg cacacgtcgc
     1861 agggcctcat gctgcccaac tcaggctcgg tgcaccggaa gcttactaac atcctccaca
     1921 ggctcgtcgc tgccgtagct cgaccgggcc gcgtagaagt ccagcatagt ggcgaaagac
     1981 cgcgcggtga ccgccccgta cagcaggccg cccagcccca cggccaggat tattatctcc
     2041 acgccacccg ttataccgcc ggtgttctcc gggtttcccc acaacgacga aaaggcgata
     2101 ccggccgtta cggccaggct cccggccagc atgttcggta ttatccaacc tgcctggggg
     2161 tctaagtggc gctggactgt cgggcgttgc gcgtagccca tcagcacgcc cgctataccc
     2221 ccggcggcta tgtaaaacag gatgcctatg atgacgatgg tgaggccgcc caccagcgtg
     2281 ccaagcaccc aggccgcgat gcctccaagc gtggtaaata gcgcccactc gcccagcagc
     2341 ggccgcccgg taaagtgctg cacgaccaac ccctgcgcca gccccaccag cagcccgtcc
     2401 agcaggccaa gcaccgcaaa gggcagcgcc aggtcgaaca gcgcggctac gccgcctgag
     2461 ccgccccagt tgatgaacag agtgactata ccgaaggcgc ttatgcccgc gatgtggccc
     2521 acggcgttag ccagtaccca gtagagccag gcgttatggg tggacggcac gttgggactg
     2581 agatttgagg cctggttggt ggtttgttga agggacatat tgggtgctgc ttacagggtg
     2641 ataaggatat tagaattcat cagtgcgggt gtaccgggca accacgcgga cattatatta
     2701 tggcacataa ccttgcaaag tagctatcaa tttcgacggt ccccgtattt ttctgggtgc
     2761 gacactatga gtccctcgct catggtgccg gtgtggcagg ggcagtggca ggaccgggca
     2821 gactccaggc gttgaggtaa gccccggagg tccagtagac gaagccgtgg gccaccgaag
     2881 gccccgtcca cgacggccct gcatcctggg gttcgaccgc ccgtagcagc gcgccggtat
     2941 gctcgtcgta gacgtgcaac acgcccgtca ggttcaggaa tacgaggccg ttggcgattg
     3001 ccatgctgcc gaatgggtag tcgggcaggg ttgcgggcgt ccagcggtcc gccccggtgg
     3061 cggggtccac tgcgtacaac tggatgccgt tatccttatg gcccacaaag aagagcgtac
     3121 cgccaggccc gagggtcggg tcgtaagccg gagtcatggc aaccgctgat tttacctccc
     3181 tgccccagat aggaccgccg ccgatgctgt cgaggttata ggcgcggaag gtgccgtcct
     3241 tgtggctcgc ggctgccagt gtgcggccct ggctatcgct gatgatgacg ggagtgctgc
     3301 cccaatcgtg gtccacgttc agggagcctt gcttgtcgga ctgttttacc tcgaggctaa
     3361 gggcgtccaa cgagatgagc gcgcggttga gcggcccatc gtagccctca aagtcctcgc
     3421 cggtggcaac tagcagggtc ctggcgtcgg gggtgaggga gggcgcgtgc cagactccgc
     3481 cccctgactt gccctcaggc acgataaaac gattcgccac gagcctgccg ctccccaggt
     3541 caacgctgcg cacctcgccg cgcacggagg gcctgtcgca gtaggaggcc acgccgtagt
     3601 aagccctgcc gtgggcgagc aggggggagg cccaggcgaa gccgctcggc cccacgttga
     3661 gagggtcgcg ccagagcgtc gcgcccgtgt ccgcattcag gccgtaaaaa gcggcatcgg
     3721 ccccgcccac gacggctata gaacccgaaa tcgcggccgt tgagccgata cccacgtcga
     3781 tgcttacgtc cgcgcaatct tccggagcgt caccgaggtt gatttgccac agggggacgc
     3841 cggtggaggc atcgaacgca aagaagttag gcccctcgtc ggcgctgccg cctatgtaga
     3901 ggcggccgtt tgccacgctg ggcgcgctac ccgaaggtat cctctcaggg tttaccaccg
     3961 cacgccagcg cggggccagg tcgcctatat tggcggcggt tagcgtttgt tcgtccgggt
     4021 tgaagttggt acggctcaag tcgcgcccca gcatgggcca atcgcctggg gcacccggtc
     4081 gtatggcgct cataccggga ttcacgttcc ggccgatgtc cggggcagtg gtgggtgtgc
     4141 ccgctcgtgt agctacatag accagcacag cgaccaccgc tagcaatagg atagatgata
     4201 caaagattat ggagtacttg gatcgcatat cattccgcgc ctgcacgcgt gaggacggtc
     4261 caccggcgtg ggggcaagtg gacggaatcg ctcaaaaaca agcggataag gtccatcccg
     4321 gcgcaacagg gtagtgggtg cctagccttc cctgtccagc gactgcacga gcttctctac
     4381 ctgctggggg acggggttga agggatcgcg caggacgata gtctgctcgc ggtgggggcc
     4441 gaccgacacc atgtcgatgg gtgtctggag gatggcggtg agccggtcga ggtaatgccg
     4501 cgcgttgtcg ggcaggtcgg accacttgcg ggcacccgac gtatcggtca gccagccggg
     4561 gtactcgtcg tactgcggct cgacttgcgc cagcatctcg atgtcggcgg gcatgtggtt
     4621 aaggtgggcg tcgttcacct tgtaggcggt gcaaatcctg atagtctcca tctggtcgag
     4681 agcgtcgagg cgggtaatcg caagccccgt gaggccgttg aactggcaca cgtacctccc
     4741 ggctaccgcg tcgaaccagc ccacccgccg ggggcggccc gttaccgtgc cgtactcgcc
     4801 gtgccccttg ccgccttgct cgcgcaggaa agtggcaagc tcgccctgta tctcggtggg
     4861 gaacggcccc gcgcccaccc gcgtctggta cgccttgaac acgcccacta tggagctaag
     4921 ctggttgggc ggcgtgccgg agccgacgca cagccccgcg gcggtggggt tgctcgacgt
     4981 tacgtagggg taggtgccga aatcgaggtc gaggagggct gcctgcgcgc cttccaagag
     5041 cacgctacgg ttgtcggtta tagcgcgggt gataacaagc tcggtgggca tgatgtgctt
     5101 gcgcaggcgc tggccctgct ccacgagccg caggtacgtc ttgtgcacgt cgataggggt
     5161 ggtgttgtag acctgcgaca ggagggcgtt cttggccggt atgaccgagg agagcttggt
     5221 gagcagcgac tcgtcgttca gcaggtcgcc catgcggatg cccacgcggg cggtgcggtc
     5281 catgtaggcg gggccgacgc cccggcgcgt ggtgcctatc ggcccggtgc cggtgccctc
     5341 tcgtgcgtct tcttccaggc cgtcgagtag cttgtggtag ggcatgatga cgtgagcgcg
     5401 gtcgctgatg agcaggcgcg acacgtccac gcccgccgct gtgaggctgt ccatctcgtc
     5461 gagcagcacc tcagggtcta tcaccacgcc gttgccgatg atgcacacgg cctgggggtt
     5521 gaagataccg gagggggtga ggcggaggcg gaaggtgcca agctcgttga caatggtgtg
     5581 cccggcgtta gtgccgccgt tgcagcgcgc caccacgcct acctgctcgg ccagcaggtc
     5641 cacgatcttg cctttacctt cgtcgcccca ctggcaacct atgactgcta ctactgccat
     5701 gtcgctccgc taagtaatga ggaatgagga ctgaggactg agtgacgttg cacctgtaag
     5761 ctagatttca gactttttgg gagtcgcgtt gcttttctac agaagacctc aagccgccta
     5821 ttatcctgcc tacttcttcg gcttgcaaca ttatctgcct gaaagtgtct tccccaatat
     5881 agcctatgtc aagagctacg tatagctgcg accgtaattc agcgcatgaa gacttggcaa
     5941 tagagagaaa gtggtggaac tctgccctgc tgccgcgttc gaagccctca gcgagattgg
     6001 acatgataga gacggcagca cgctgaatct gacctgaaag actgaagtcc ttagcaaacc
     6061 ttccttgttg cgttactgcg tagatgtccc tggtaaggac tctggccttc tgccaggcaa
     6121 ccaaatcctc aaaacgtcca atcttggctt gcatcaggta tcactcagtc ctcagtcctc
     6181 agtcctcatt acttacttca caacccgcag atggtcgcga cctgatgcct gctttggtgg
     6241 tctacctctg cgtcgctggg cgggctgctg gtcctccgcg cctgctgcca gatcgctggt
     6301 gatttcggct ccggggtcgg gcggttctga ctggtcgccc ttcttggcgc ggcctttgcg
     6361 cttcgacttg gcttcgcctt cctcgtcgtc tgctgcgggt ttggtgcggc tggcgatcag
     6421 ggcctccacg tcgaagtcgc cggagagctt cttttcgtcg aattcggctt gcatctgcgc
     6481 cagcttctca gcgaggccgg gcactacctc ggcggcatcg acgatattat acctgtaacc
     6541 ctgctctgtc aggaatagct gccggttagt agaaaagtcc tggtccttgg tgtcgcgggt
     6601 gacgagcgag tagaagttgg cgatggagcc gtccttcttg gggcggagga tgcggcccag
     6661 gcgttgcgcc tcctcctgcc gcgagccgaa ggtgccggat acctgtatgg ccacgttggc
     6721 gtcgggcagg tctatcgcga agttggctac cttgctcacc accagcatct ttagctgtcc
     6781 gctacggaac tgctcgtaca gcctgtcgcg ctccacgttg ggcgtcttgc ccgtgacgat
     6841 gggtgcctcg aaccgcttgc tgatctcctt tagctgctcg atgtactgcc cgatgacaag
     6901 caccgagtcg gcctggtgcc gttccaggag cacttccaac gcgtccatct tgatggggtt
     6961 ctcggcggcg
//
LOCUS       DASRWZ010000433         1772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1201689, whole genome shotgun sequence.
ACCESSION   DASRWZ010000433 DASRWZ010000000
VERSION     DASRWZ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201689"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..641
                     /locus_tag="VFH60_06785"
     CDS             51..641
                     /locus_tag="VFH60_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET6313523.1"
                     /translation="MATKPTPYDCLEAFDAESGDLNVIIETPKGSRNKFDYEPKLGIF
                     KLGGVLPAGAVFPYDFGFVPATLGGDGDPMDVLVLMDEPAFTGCLVPSRLIGVIEAEQ
                     TERDGETTRNDRLIAVAANSHNHQDVHTLNDVTTNLVDEIEHFFISYNQAKGKEFKPL
                     KRSGPTRARHLVEEGIEQAKQPDSSGKKALGQSKAK"
     gene            658..>1772
                     /locus_tag="VFH60_06790"
     CDS             658..>1772
                     /locus_tag="VFH60_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET6313524.1"
                     /translation="MWQTYFQPTTIEEALRLLQEHAGEARIVSGGTDVLVELQRGIKP
                     TSTLIDITNLRDLKYVRVEGGTVRIGGLASHNDVLASPECIAAALPLAQACIEIGAPQ
                     IRTRGTIAGNLLTASPANDTIAPLMVLGAEVVLVSLSGERVILLDSFYQGVRRTAIAP
                     DELLREIRFPALTEKRRGMWMRLGLRRAQAISVIHFAIVLTFEGEQVSQASITLGCVA
                     PVIVHAREAEEYLVGKRLDPAVCAEAGRLACAGIKPISDLRGSAQYRLTTLAALVTQG
                     LQSIAAGTEATGFPTRPVLLDTGSDPHNMAEFAGVIDTTINGKRLQLPDAQHKSLLHA
                     LREDAGLTGTHEGCAEGECGACTVWLDGQAVMSCLVPA"
BASE COUNT          373 a          564 c          546 g          289 t
ORIGIN      
        1 tgggtgcgcg tgctgcgtaa gacctggcaa gaatagagag ggcgacacta ttggcgacca
       61 aacctacccc gtatgactgc ctggaagcgt tcgacgccga gtcgggcgat ctaaacgtga
      121 taatagagac gccaaaggga agtcgcaaca agtttgatta tgaaccgaag ctgggcatct
      181 tcaagttggg gggagtgcta ccggcgggcg cggtcttccc ctacgatttc ggcttcgtac
      241 ccgccacgct gggaggcgac ggcgacccta tggacgtgct ggttctgatg gacgagcctg
      301 ccttcaccgg ttgccttgtg ccctcccgct tgatcggcgt gattgaggcc gagcagaccg
      361 agcgcgacgg cgagaccacg cgcaacgacc gcctgatcgc cgtcgcagca aactcgcaca
      421 accaccagga tgtccacacg ctcaacgacg tcaccaccaa ccttgttgac gagatagagc
      481 acttcttcat ctcttacaac caggcgaaag gcaaggagtt caaacccctc aaacgctccg
      541 gccccacccg tgccaggcac cttgttgaag agggaataga gcaggcgaaa caacccgact
      601 cttccggcaa aaaggcatta ggacagagca aggcgaaata ggcgacagga gtttgcgatg
      661 tggcaaacct acttccaacc caccacgatt gaggaggcgc tgcgcctgct gcaagagcat
      721 gcaggagagg cgcgcatcgt gtcgggcggc accgacgtgc tggtggagct tcagcgcggc
      781 atcaagccta catccaccct catagacatc accaacctgc gcgatctcaa gtacgtgcgc
      841 gtggagggtg gcaccgtgcg catcggcggg ctagcctccc acaacgacgt gctggcctcg
      901 ccggagtgca tagccgccgc cttgcccctg gcacaagcct gcatagagat aggcgcgcca
      961 cagatacgca cgcggggcac catcgcgggc aacctgctta cggcttcgcc cgccaacgac
     1021 actatcgccc cgctcatggt gctgggggcc gaggttgtgc tggtcagcct atcgggagag
     1081 cgcgttatac tcctggatag cttctaccag ggggtgcgcc gcacggctat agcgcccgac
     1141 gaactgctac gcgaaataag gttccccgcc ctcacagaga agcgcagggg catgtggatg
     1201 agattgggat tgcgccgcgc ccaggccatc tccgtgatcc acttcgccat cgtcctcacc
     1261 ttcgagggcg agcaggtgag ccaggcaagt attacgctag gctgcgtggc ccctgtaatc
     1321 gtgcatgcca gagaggcaga ggagtatttg gtaggcaaga ggctcgaccc ggcggtctgt
     1381 gccgaagcgg gaaggctcgc ctgcgcgggc atcaagccta tcagcgacct gcgcggctcc
     1441 gcccaatacc gcctcaccac cctggccgcg cttgttacgc aggggctgca aagcatagcg
     1501 gcgggcacgg aggccacagg cttccctacc cggcccgtgc tgctcgatac gggttcagat
     1561 ccgcacaaca tggccgagtt cgcgggcgtg atagacacca ccatcaacgg caagcgcttg
     1621 cagctacccg acgcccaaca taagtcgctc ctccacgccc tgcgcgaaga cgccgggctg
     1681 accggcacac acgagggctg cgccgagggg gagtgcggag cctgcacggt ctggctcgac
     1741 ggacaggcag tcatgtcctg cctggtgccc gc
//
LOCUS       DASRWZ010000434         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_310715, whole genome shotgun sequence.
ACCESSION   DASRWZ010000434 DASRWZ010000000
VERSION     DASRWZ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310715"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFH60_06795"
     CDS             <1..292
                     /locus_tag="VFH60_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313525.1"
                     /translation="ELAPSMLGRQFLIPVFDQTNGRGGNNTKFHLIGLAWFEITDADI
                     QCHKNEFVPGTPTPLDEHWAFEGKFVNVYTAGATGSHGDIRHTSQHVVFLEP"
     gene            complement(375..2012)
                     /locus_tag="VFH60_06800"
     CDS             complement(375..2012)
                     /locus_tag="VFH60_06800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6313526.1"
                     /translation="MAKATQPDVAPHVGADEAESLSPQSSVLITSPGKHITIAALGIF
                     LAALDQTVIVTALWPISTDLQIPVTELDRAAWVVTAYLLGYTVALPLMGRVADVYGQR
                     RIYLVSLGIFMLGSILCALAPSLEFLIAARALQAIGGGAVLPVGMAMVRHLFREHRVP
                     FMLGVLGAVAEAGGVIGPLWGALVMTGFDGWFGVIGWRWIFWVNVPLCLLFAFLIFRT
                     PPLPRYPGKVDWAGAGLLGMALLALSLGLSTSGTVGAWAGLDPQTTAGASGELITPQS
                     LTLFGLSLVLFVAFVLWQRRASTPLIPLELFSRRTWPFSAANITNFMVGAALIITMVN
                     VPLYVASVLDGTAEQGGFMLLRMTALIPVGAIAGGLLGVRVQFRWVAVLGLIVTALGF
                     WEMSAWSLAAPDAPLNWLGLALNGLGFGLLISPVTATALQWGGLKRAALSAASVNVSR
                     MIGMMVSLSVLTVLGLHRFQELMSAHPAPIVPFRDETAEQFAQRTADYTVAYKSASLE
                     VYTTGFLIALLVCVAGIAFALWLRRNPESEVETGPRF"
     gene            complement(2082..3107)
                     /locus_tag="VFH60_06805"
     CDS             complement(2082..3107)
                     /locus_tag="VFH60_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET6313527.1"
                     /translation="MNKITIIGLGLVGNSIGMGIKRAAAQTASDATAPSAQGVHVVGF
                     DPGRERESAALRRYNSVDEIAPDLRRAVQGAELVVLATPSGAVREVMEAIAPFLEEGA
                     TVTDTLPSKEQVMSWAAELFGGRVGFVGGHPMSKSLDLSLASDESLPSPDLFYKAHYC
                     ILPSPRAGNEALNRVIWLAEILGAQPLFIDTLEHDSFVAAASQLPALASANLLRITAG
                     GSTWTDMSVFAGEQYDAVIEPLEADPERLTESLLQNRQALVRWLDQYILSMHELREML
                     ARSNRDDLLDAMQRAHASQASHTNRNERDNELRSELRQAIQETHPVRGLLGGYISDRV
                     FRKKEGK"
BASE COUNT          621 a         1054 c         1041 g          508 t
ORIGIN      
        1 ggagctagcc ccctcgatgt tgggccgcca gttcctgatc cccgtgttcg accagaccaa
       61 cggcaggggc ggcaacaaca ccaagttcca cctgataggg ctggcctggt tcgaaatcac
      121 cgacgccgac atccagtgcc acaagaacga gttcgttccc ggcacgccca cccccctgga
      181 cgagcactgg gccttcgagg gcaagttcgt caacgtgtac accgcagggg ccaccggcag
      241 ccacggcgac atccggcata cctcgcagca cgtggtgttc ctggaaccgt gacggaattt
      301 gggaattggg atttcgaatt tcggatttgc tcggatatca actaaatccg caatccgaaa
      361 ttccaatttc caaatcaaaa tctcggtccc gtttccacct cggactccgg gttgcggcgc
      421 agccagaggg cgaacgctat gcccgcgacg cagacgagca gggctatgag gaagccggtg
      481 gtatacacct ccagagaggc cgacttgtag gccaccgtgt aatcggctgt gcgctgggcg
      541 aactgctcgg cggtctcgtc gcggaagggc acgatgggcg cggggtgggc agacatcagc
      601 tcctggaagc ggtgcaggcc gagcacggtg agtaccgaca ggctcaccat catccctatc
      661 atcctcgaca cgttgacaga ggctgcggat agggccgctc gcttgaggcc gccccactgt
      721 agggccgtgg cggtcacggg cgagatcagc aggccgaagc cgaggccatt gagcgccaac
      781 cccagccagt tgaggggcgc atcgggcgct gccaggctcc acgcgctcat ctcccagaag
      841 cccagcgcgg tgacgatgag gccgagcacc gccacccacc ggaactgcac ccgcaccccc
      901 agcaagccgc ccgctatcgc ccccaccggg atgagggccg tcatcctcag cagcataaag
      961 ccgccctgct cagccgtgcc atccagcacg cttgcaacgt acaggggcac gttcaccatc
     1021 gtgatgatga gggcggcgcc caccatgaag ttggtgatgt tggccgccga gaagggccag
     1081 gtgcgccggg agaagagttc cagcgggatg aggggagtgg aggcccgccg ctgccagagc
     1141 acgaaggcca cgaacagcac gagcgagagg ccgaacaggg tcaggctttg gggcgtgata
     1201 agctcgcccg acgcacccgc ggtggtctgc gggtccaggc ccgcccacgc gcccaccgtg
     1261 cccgacgtgg agaggcccag cgacagggcc agcagcgcca tgcccagcag cccggccccc
     1321 gcccagtcca ccttgccggg gtagcgaggc aggggaggcg tgcggaatat gaggaaggcg
     1381 aacagcaggc agagggggac gttcacccag aagatccacc gccagccgat caccccgaac
     1441 cagccgtcga agcccgtcat cacgagcgcg ccccacagag ggccgattac gccgcctgcc
     1501 tcggccaccg ccccaagcac gcccagcatg aagggcaccc ggtgctcgcg gaagaggtgg
     1561 cgcaccatcg ccatgcccac cggcagcacc gccccgcccc cgatagcctg caacgcccgc
     1621 gccgcgatta ggaactcaag cgagggggcc agcgcgcaca ggatcgagcc gagcatgaag
     1681 atgcccagcg acaccaaata gatgcgcctc tgcccgtaca cgtcggccac gcgccccatg
     1741 agcggcaacg ccaccgtgta acccagcaga taggccgtga ccacccaggc cgcgcggtca
     1801 agctcggtaa cgggaatttg caggtcggtg gaaattggcc agagggccgt gacgatcacc
     1861 gtctggtcga gggccgccag gaagatgccg agcgcggcga tggtgatgtg cttgccgggg
     1921 gaagtaatga ggactgagga ctgaggactg agtgattcgg cctcatcggc tccaacgtgc
     1981 ggagccacat caggctgtgt tgctttagcc atgcgccaac ctcacccgtg tctactcagc
     2041 acccactcag tcctcggtcc tcagtcctcg gtcctcatta cttacttccc ctctttcttg
     2101 cggaagaccc tgtcggagat gtagccgccg aggaggccgc gcacggggtg ggtctcctgg
     2161 atggcctggc gcagttcgga gcggagttcg ttgtcgcgct cgttgcggtt ggtgtggctt
     2221 gcctgcgagg cgtgggcgcg ctgcatggcg tccagcaggt cgtcgcggtt gctgcgagcc
     2281 agcatctccc gcagctcgtg catcgacagg atgtactggt cgagccagcg tacgagggcc
     2341 tggcggttct gcaacaggct ctcggtaagc cgctcagggt cggcctccag cggctcaata
     2401 acggcgtcgt actgctcgcc cgcgaacacg ctcatgtcgg tccatgtgga cccgcccgcc
     2461 gtgatgcgca gcaggttggc gctggcgagg gcgggcagtt gggaggccgc tgccacgaac
     2521 gagtcgtgtt ccagcgtgtc gatgaacagc ggctgcgcgc ccagtatctc ggccagccag
     2581 atcacccggt tcagcgcctc gttacccgcg cggggggagg gcaggatgca gtagtgggcc
     2641 ttgtaaaaga ggtcggggct gggcagggac tcgtcgctcg cgagggagag gtccagcgat
     2701 ttcgacatcg ggtgtcctcc cacgaagccc acacggccgc cgaatagctc cgccgcccac
     2761 gacatgacct gctccttgga cggcagggtg tcggtgacgg tagctccctc ttccaggaat
     2821 ggggcaatcg cttccatgac ctcgcgcacc gcgccggaag gggtagccag caccacaagc
     2881 tcggcaccct gcacggcacg ccgcaggtcg ggggcgatct cgtccacgct gttgtagcgg
     2941 cgcagggctg ccgactcgcg ttctcggccg gggtcgaacc cgacgacgtg cacgccctga
     3001 gcggaggggg ccgtagcgtc gctagcagtc tgcgcggccg cccgcttgat ccccatgcca
     3061 atagagttgc ctaccaggcc gaggccgatg atggtgatct tgttcataat gaagtataca
     3121 ccgattttgg attttagatt tcggatttga catcgttaag ctacttacaa caattcgggc
     3181 ctgccagttc acccccgaca atccaaaatc caaaatccaa aatc
//
LOCUS       DASRWZ010000435         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1064464, whole genome shotgun sequence.
ACCESSION   DASRWZ010000435 DASRWZ010000000
VERSION     DASRWZ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064464"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1002)
                     /locus_tag="VFH60_06810"
     CDS             complement(163..1002)
                     /locus_tag="VFH60_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6313528.1"
                     /translation="MPTLDFEERGEGTPLVLVHGFPFDRSIWRAQLEGLSDEARVIAL
                     DLPGFGGSRPLEGDEASIDGYADAISRWAGEVGLDRFVIAGHSMGGYVALAFARRHPE
                     MLAGLGLVCTRPGPDTEQARQGRYTMIDNVREKGPQAVVDAMLPKLLSPSTREEQPGV
                     EEQTREVMLHQSSDGIIAALQAMASRPDSSPMLAQIEVPTAVIGGADDAIIPAAEEDL
                     IAATIPGATQIRIDGAGHMPMLEQPEKLTVALRNLARGSRTTPAIPTPADPHNIEGVR
                     RHD"
     gene            complement(1151..2107)
                     /locus_tag="VFH60_06815"
     CDS             complement(1151..2107)
                     /locus_tag="VFH60_06815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6313529.1"
                     /translation="MAVIGYWLSSEEHTPNDLVEYARQAEELGFTHIMISDHYHPWVD
                     AQGHAPFVWSVIGGIASVTKKVTLGTGVTCPTIRTHPAIIAQAAATAAAMMPGRFILG
                     LGTGENLNEHILGDKWPPFQERLEMLEESIEIMRKLWEGKITSHRGEYYTVENARIYT
                     LPEQPIPIYIAAAGTDTAEVAGRMADGFINTSPEKAPLEAFEQAGGAGKEKHGQFTCC
                     WAETEQEAVRTAYKIWPNGGLKGPLSQELPLPQHFEQAVKMVSEEDIAESVVCGPDPE
                     PYLAKIREYADAGFTHIYLHQVGPDQEGFFRFYQRELAPKLG"
BASE COUNT          372 a          723 c          777 g          499 t
ORIGIN      
        1 gcaaagacgc aaaggactca aaggacgcgc aaagagtagt aatgcttaat gtgtgcaaaa
       61 aggagcaggg tacatgatgt cgtaccctgc tccttttgct tagaacacca ccctagtctt
      121 ggcgtggtcc ttggcgtctt ggcgtcttgg cgtttccgtc tcctaatcgt gtcgccgcac
      181 gccttcgatg ttgtgagggt cggcgggggt gggtatggct ggggtggtgc gggagcctcg
      241 ggccaagttg cggagggcca cggtgagctt ttcgggctgt tccagcatcg gcatgtggcc
      301 cgcgccgtct atgcgtatct gggtggcccc cggtatggtg gccgctatga ggtcttcctc
      361 ggcggctggg atgatggcgt cgtcggcacc tcctatgacg gcggtgggca cctctatctg
      421 tgccagcatg ggggaggagt cggggcggga ggccatagct tgcagggcgg cgattatgcc
      481 atcgctgctc tggtgcagca tcacctcgcg ggtctgctcc tccacgcctg gttgctcctc
      541 ccgcgtggat ggggacagca gtttgggcag catggcatcg accacagcct gcggcccctt
      601 ctcccgcacg ttgtcgatca tggtgtagcg gccctgcctg gcctgttccg tgtcggggcc
      661 ggggcgggtg cataccaggc cgaggcccgc cagcatctca gggtgccttc tagcgaacgc
      721 cagcgccacg tacccgccca tcgaatgccc cgcgataaca aacctgtcca gcccgacctc
      781 cccggcccag cgagatatgg catcggcata accgtcgatg gaagcctcat cgccctccag
      841 gggccgcgac ccgccgaacc ccggcaagtc gagggcgatc acccgcgcct catcgctcaa
      901 cccttccagt tgcgctcgcc agatcgagcg gtcgaacggg aagccgtgca ccagcacaag
      961 cggcgtgcct tcgccgcgct cttcaaagtc cagtgttggc attggtgtaa ctccttcggt
     1021 ggacgatgag tggtcagtgg tcggtggtca gtatccgagg cgtccaacct ctctgcggat
     1081 ccggcgggat ctttgtgcct ttgcggttca tttcttcacc gaccaccggc cactgaccac
     1141 cggccactca ttatcccaac ttgggtgcaa gctcccgctg gtagaagcgg aagaagcctt
     1201 cctggtcggg gccgacctgg tggaggtaga tgtgggtgaa gcccgcatcg gcgtactccc
     1261 gtatcttggc gaggtagggt tcggggtccg ggccgcacac tacggactcg gctatgtctt
     1321 cctcgctcac catcttgacg gcctgctcga agtgttgggg gaggggtagc tcctgggaga
     1381 gcggaccttt cagcccaccg ttgggccaga ttttgtaggc ggtacgcacg gcttcctgct
     1441 cggtctcggc ccagcagcag gtgaactgcc cgtgcttctc tttgcccgcc ccgcctgctt
     1501 gctcgaaagc ctccagcggc gccttctccg gcgacgtgtt gatgaagccg tcagccatgc
     1561 gcccggccac ctcggcggta tcggtcccgg cggcggctat gtagatgggt atgggttgtt
     1621 cgggcagggt gtagatgcgg gcgttctcca cggtgtagta ctcgcccctg tggctggtga
     1681 ttttaccctc ccagagcttg cgcataatct cgatggactc ttccagcatc tccaacctct
     1741 cctggaacgg gggccacttg tcgcccagga tgtgctcgtt gaggttttcg cccgtgccca
     1801 ggcccaggat gaagcggccg ggcatcatgg cggcggcggt ggcggcggcc tgcgcgatta
     1861 tcgcggggtg ggtgcgtata gtggggcatg tcacgccggt gccgagtgtc actttcttgg
     1921 tgacgctggc gatgccgcct atgacgctcc acacgaacgg cgcgtggccc tgcgcgtcca
     1981 cccaggggtg gtagtggtcc gagatcatga tgtgcgtgaa gcccagttcc tcggcctgcc
     2041 gggcgtactc caccaggtcg ttaggggtat gttcctcgct cgacagccag taaccgatta
     2101 cagccatgag tgctcctccg atgtgaaagc tgattagttc gtgcccggca tgaagtcacc
     2161 ggcgaggggg atgcggcaag atacgtgccg aaccctgcct gcactggggt tggcggctgc
     2221 cacctcgcaa atgttccggc tggagcgccc caagcccgcc cctgtcgtcc agagcttccc
     2281 cttgtcatcc tgagcgtagc gaaggatcag gtgggtaggc aagagtcttc aacctaggga
     2341 tgctggcggt aagaggaaca ttggtgctca a
//
LOCUS       DASRWZ010000436         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1133125, whole genome shotgun sequence.
ACCESSION   DASRWZ010000436 DASRWZ010000000
VERSION     DASRWZ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133125"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1156)
                     /locus_tag="VFH60_06820"
     CDS             complement(47..1156)
                     /locus_tag="VFH60_06820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016953564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET6313530.1"
                     /translation="MLDDNPIQRFTNLVELEALAREVLEPSAFDFISGGADDEVSLRR
                     NRADFERIILRPRVLVDVSEVDCSTTVLGTPVSMPVLLAPAAGHRLCCDLGESATARA
                     AAAARTIMVLSTVSTTSMEDVAAVEPDAPKWFQLYVYADREVTRGLVQRAEAAGYKAL
                     CLTVDVPQIGNRERDVRNAFTFPYPLANFAATNLDHLPIGMSGEESGLGKYIASKWDP
                     SLNWNDVDWLASLSSLPVVLKGILTGEDARLGLEHGARGIIVSNHGGRQLDSVDSGIA
                     ALPEVVEAVGGRAEVLVDGGIRRGTDVLKAVALGAKATLIARPYLYGLAVGGAEGATR
                     ALELLRREVRNSMALSGRPTVASIDRSLVKFRPDG"
     gene            complement(1197..2174)
                     /locus_tag="VFH60_06825"
     CDS             complement(1197..2174)
                     /locus_tag="VFH60_06825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET6313531.1"
                     /translation="MNERPVIRPETVSDYAAVADTNVQAFDNRAAEALIVTLLRNRPR
                     FDPELSLVAEADGHVVGHVLFSPYDVQVLGESVPAVNLAPIAVRPEYQKQGIGGRLIE
                     AGHELARARGYRFSLLVGHPTYYPRLGYKVRAYGNSSLKLPTASDAFASLLDERVGLE
                     VRGPQARDVPSLRALWLREERLVDFSLDPGTELLDWLSPSPLVETLVFLRDEQVAGYV
                     RARTGKSGAVLVFLASDAAVARSMVAELYIRRRESGVPPDEISLPLHPSSGSVAAFAD
                     FGTPQGHAFVAAMAFPLVPSLFDEYYARTQSGERPAGRLIWPSIFDIAL"
     gene            2372..>2574
                     /locus_tag="VFH60_06830"
     CDS             2372..>2574
                     /locus_tag="VFH60_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313532.1"
                     /translation="MLKDEILRYAQNDSRGAGQPQATLKNGRLILHYVQDDKGHMVPP
                     SDLDTTLTPTRAVLTPLEQQPYNT"
BASE COUNT          486 a          855 c          805 g          428 t
ORIGIN      
        1 gagggtcccg cgacgccaac tgtcgcacat cgatgcaccg gcctcgtcac ccatcaggcc
       61 taaacttcac caggctgcgg tcgatgctcg caactgtggg gcggcctgag agggccattg
      121 agttgcggac ttcgcggcgg agtagctcca gcgcgcgggt agcgccttcc gcgccgccga
      181 ccgccaggcc gtagaggtag gggcgggcta tgagcgtggc cttcgcgccg agcgccacgg
      241 ccttcagcac gtcggtgccg cgcctgatac cgccgtccac cagcacctcg gcgcggccgc
      301 ccaccgcctc caccacttca ggtagggccg ctatgccgga gtccaccgag tctagctgcc
      361 gcccgccgtg gttgctgacg atgatgcccc tcgcgccatg ttcgaggccc aggcgggcgt
      421 cctctcccgt cagtatgccc ttgagcacca ccggcaggct gctaagggaa gccagccagt
      481 ccacgtcgtt ccagttgagc gaagggtccc acttggaggc tatgtacttg cccaggcccg
      541 actcctcgcc cgacatgccg ataggcaagt ggtccaggtt ggtggcggcg aagttggcga
      601 gggggtaggg gaaggtgaaa gcgttacgca cgtcgcgctc tcggttgccg atctggggca
      661 cgtccaccgt gaggcagagc gccttgtacc cggcggcctc ggcacgctgc accaggccgc
      721 gggtcacctc ccggtcggcg tacacgtaga gctggaacca tttcggggcg tccggctcca
      781 ccgccgctac gtcctccatg ctggtagtgg acacggtgga gagcaccatg atcgtgcggg
      841 cggcagcggc ggcgcgtgcg gtggcgctct cccccaggtc gcagcagagg cggtgcccgg
      901 cggcgggcgc gagcagcacc ggcatgctca ccggcgtgcc cagcaccgta gtagagcagt
      961 ccacctcgct cacgtccacc agcacgcggg gccgcaggat gatgcgctcg aaatcggccc
     1021 ggttgcggcg caggctgacc tcatcgtccg caccgcctga gataaagtcg aatgcggacg
     1081 gttccagcac ctcgcgggcc agcgcctcaa gctctacaag gttggtgaat cgctgtatgg
     1141 ggttatcatc taacatagct gtaatgtgcc tgcttctgca aagggtagct gtccgattat
     1201 aaagctatat cgaaaatgct cggccagatc aagcggcccg ccggacgctc gcccgactgc
     1261 gtgcgagcgt agtactcgtc aaacaggctg ggcaccaggg gaaaggccat cgcggcgaca
     1321 aaagcgtggc cctgcggagt gccgaagtcc gcaaacgccg ctacgctgcc ggaggatggg
     1381 tggagaggca agctaatctc gtccggcggc accccgctct cccgcctcct gatgtatagc
     1441 tcggccacca tagagcgcgc aaccgccgcg tctgacgcca ggaacaccag cacggcaccc
     1501 gacttgccgg tacgcgcccg tacgtagccc gccacttgct cgtcccgcag gaacaccagc
     1561 gtctccacca gcggagatgg gctgagccag tccagcagct ccgtgccggg gtccagcgag
     1621 aagtccacca ggcgctcctc ccgcagccac agggcgcgta gcgacggcac gtcccgcgcc
     1681 tgtggccccc gcacctcgag gccaacacgc tcgtcaagca gggaggcaaa ggcatcgctg
     1741 gccgtaggca gcttgagaga tgagttgccg taagcgcgca ctttgtagcc caggcgcggg
     1801 tagtaagtgg gatggcccac aaggaggctg aaccggtagc ccctggcccg tgccagctca
     1861 tgccccgcct ctatgagcct gccgccaatg ccctgcttct ggtattcggg gcgcaccgcg
     1921 attggcgcga ggttgacggc tggcacgctc tcccccagca cctgcacgtc gtagggcgag
     1981 aatagcacgt ggcccactac gtgcccgtcc gcctcggcca ccaacgacag ttcagggtcg
     2041 aaccgggggc gattgcggag caaggtgaca atcagtgcct cggcggcacg gttgtcaaac
     2101 gcctgcacgt tggtatcggc aacggcggcg tagtcgctca cggtttcggg gcgtatcact
     2161 ggacgttcgt tcattaagcc tctttccttc gacgcctgca tctatgaccg ctactgtgcc
     2221 aactcgcaac gtaccaaagc agaatgacgg aaccaagccc cgcctttgtc attctgagcg
     2281 tagcgaagaa tctcgtcctc caccggaatg cagccctttt ctatggcacc aaagttcctc
     2341 acgaagtagc ctaaggaaag gtggtacttg ggtgctgaag gacgagattc ttcgctacgc
     2401 tcagaatgac agtaggggag cgggtcagcc acaagcaaca ctcaagaacg ggcggctgat
     2461 ccttcactac gttcaggatg acaaggggca catggtcccg ccatccgacc ttgacaccac
     2521 cttaactcca accagagcag tattgacacc cctagaacag caaccgtata atac
//
LOCUS       DASRWZ010000437         4063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_585385, whole genome shotgun sequence.
ACCESSION   DASRWZ010000437 DASRWZ010000000
VERSION     DASRWZ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585385"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VFH60_06835"
     CDS             <1..1202
                     /locus_tag="VFH60_06835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313533.1"
                     /translation="TGVTRQFRQWPEAVLGRALVAKGHTVAAFTLREEDSAITSEARE
                     QVDGIEVFRLPVNKAWLAPGLLPALTAFRPDVVHLFHLRNALNWQAALWAASMQRPIV
                     FTVVGPFHDPYLVDDRERPYLGKMYPGRLIYTPARLVRELVRARGKKPLSIWQNFCMH
                     IPLRAANRLIALSAHEVGVLKRLGIAERKIAMIPLWVDVPYIESVPYQGSVAANYTSP
                     HVLFLGQLKERKGYDTLARAMPLVLEKCPTTTFLFAGQNPARAGHLEAICRENGSIES
                     MVLLGQVDEESKVRLMRSADCLVYPTRYESFGLPPLEAMAAGCPVVATDLPVVSEMVR
                     DGENGLLVEPESPEALAAAIVRLLTESGLRARLVEGGRRTLSRYSEPELIGRIEALYV
                     GLCQGRM"
     gene            1623..3839
                     /locus_tag="VFH60_06840"
     CDS             1623..3839
                     /locus_tag="VFH60_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6313534.1"
                     /translation="MTYKTRLRGFGKLGALALAALALLPAALAPSGTAQGADLAQADR
                     GANSPAGDSVTLTGPGAITIPESDDYATQVQADPWDMNNIEDLDYPRNFTQPSLGGGI
                     WSATTTGSNTDQEMFVKLVEQNFATTFSYIGERDGRNYPVSTQRFTRIMFRMYTDQAG
                     PITLWWFKSPGDTAPCNNFDCRLKQAIDAQSGWHIYEATLNGDPADWANLGTVAGIRM
                     DAPREKYNNNVKFDWIRLVPDTSTPVQVTFRASGGGTVNFYLGLTSDYNAREIKIGST
                     SATANNWTWNRTGVAPGTYYIHAELNGSWSTTGPITVNAAPVLQLIAPGPLSGEDFAQ
                     ARLGQTWDGSNPNQFQLYQNLTGAPSFNGEYMQATSQPAPGSTVQDPALYWLYPYNGQ
                     NRGTIDTARYHYMTVKLWLQAPAERPGSPGNLGPRFLWSQGGDWVQTEGMYAPYNRWI
                     PMSVDLASVPKAPGSPAYGWSGPVNIFRFDPHEEDDTRQPAVNLPFFRIMSAHLMSQP
                     IANNATLVRWGKVQGTGSVNLYYDTNNSGFDGTLFASNVPIDQGLAGWNTSGLPNGTD
                     YWVYAEASDGTNTSRYYSLLPVRIDHGSASTIFVDVPTNNPFANDIANLAARNIINGY
                     GQPDGTMHFRPGATATRAQLSKMVVLGAGGTLVNPGTPSFMDVPTSSPLYTFVETAAA
                     RGIISGYTCGTRPSEPCDGSGRNYFRPNRDVTRGQISKMLSQAIGPFTGTSAPLNK"
BASE COUNT          804 a         1326 c         1243 g          690 t
ORIGIN      
        1 ccacgggtgt cacccggcaa ttccggcagt ggccggaggc ggtgcttggg cgcgccctcg
       61 tggcgaaggg gcacacggtg gcggccttca cgttgcggga ggaagactcg gccatcacgt
      121 cggaggcgcg cgagcaggta gacggcatcg aggtctttcg cctgccggtg aacaaggcgt
      181 ggctggcacc cggacttctc cccgccctca ccgccttccg gcccgacgtg gtgcacctgt
      241 tccacctgcg aaacgccctc aactggcagg ccgcactctg ggccgcctcg atgcagcggc
      301 caatcgtgtt caccgtggtc ggccccttcc acgaccccta cctggtggac gaccgcgagc
      361 ggccctacct gggcaagatg tatcccggca ggctgattta cacccccgcc cgcctggtgc
      421 gggagttggt gagggcgcga ggcaagaagc cgctctccat ctggcagaac ttctgcatgc
      481 acatccccct gcgcgccgcc aatcgcctga tagccctgtc ggcgcacgag gtgggcgtgc
      541 tcaagcggct ggggatagcg gagcggaaga tcgccatgat accgctgtgg gtggacgtgc
      601 cctacatcga atcggtgcct tatcaggggt cggtggctgc gaactacaca tcgccgcacg
      661 tgctgttcct ggggcagttg aaggagcgca aggggtacga cacgctagct cgcgccatgc
      721 cgctggtgct tgagaagtgc cccactacca ccttcctgtt cgccgggcag aaccccgcca
      781 gggcggggca cctcgaagcg atttgcaggg agaacggcag tatcgagagc atggtgctgc
      841 tggggcaggt ggacgaggaa agcaaggtcc ggctgatgcg ctccgccgac tgcctggtct
      901 atcccacgag gtacgagagc ttcggcctgc cgcccctgga agccatggcg gcggggtgtc
      961 ccgtcgttgc caccgacctg ccggtggtga gcgagatggt gcgggacggc gagaatggcc
     1021 tgctggtgga gccggaaagt cctgaggcgc tggccgcggc catagtccgc ctgctgaccg
     1081 agtcgggctt gagggcgcgc ctcgtggagg ggggccgccg cacgctgtcg cggtactcgg
     1141 agccggagct tatcggcagg attgaggcgt tgtacgtagg gctgtgtcaa ggtcgaatgt
     1201 aaagtggaga gggtgggctt tgtcattctg agcatagcga agaatctaag atgccggtcc
     1261 tgccactaag tcgaacgcca gcaccatagt acgtatcgtt gtacgcaacg ttcaacagcc
     1321 ttcctttggc accttcttta gattcttcgc tacgctcaga atgacagggg ccaccacagt
     1381 cctgctctcc cacgttggca aagtcccggc gttgtgcggg aactgctggg cgggtaacgg
     1441 tacggcccac tagcctatat gtaccggcgt gcgccaagac ctttgtccca ggcaataggc
     1501 ctgtttcggc tggctatcgt tatgcctatg ttggtacact taggagagta aatggccgat
     1561 attgtaacag actcatgcac ccatatttac taccatcacc cgtggaaagg aatacgagga
     1621 taatgactta caagacacga ctccgcggct tcggcaagct tggcgcgctg gccctcgcgg
     1681 ccctagccct cctcccggcg gcgctagccc ccagtggcac cgcgcaaggt gcggacctgg
     1741 cgcaggcgga caggggcgcg aacagccccg ccggggatag cgtcacactc accgggccgg
     1801 gggccatcac catccctgaa tctgacgact acgccaccca ggtccaggcc gacccgtggg
     1861 acatgaacaa catcgaagac ctcgactacc cccgtaactt cacccaaccc agcctcggcg
     1921 gtggcatttg gagcgcgacc actaccgggt cgaacaccga ccaggagatg ttcgtcaagc
     1981 tggtggagca gaacttcgcg acgactttct cctatatcgg ggagcgcgac gggcgcaact
     2041 acccggtctc gacccagcgc ttcacccgca tcatgttcag gatgtacacg gaccaggccg
     2101 gtcctataac gctctggtgg ttcaagagtc ctggggatac cgctccctgc aataatttcg
     2161 actgccggct caagcaagcc attgacgccc agtccggctg gcacatctat gaggcaaccc
     2221 tgaacggcga ccccgccgac tgggccaacc tgggcacggt ggcgggcatc cgcatggacg
     2281 cgccccgcga aaagtacaac aacaacgtca agttcgactg gatacgcctt gtgccggaca
     2341 cctccacgcc tgtgcaggtc actttccgcg ccagtggtgg tggcaccgtg aacttctacc
     2401 tgggcctgac gtccgactac aacgccaggg aaatcaagat tggcagcacg agcgccacgg
     2461 ctaacaactg gacgtggaac cgcaccggcg tggcccccgg cacttactac attcacgccg
     2521 agctaaacgg cagttggagc accacaggcc ctatcaccgt caacgccgcg ccggtcctgc
     2581 aactgatagc cccagggccg ctgagcggtg aggacttcgc gcaggcccgc ctggggcaga
     2641 cctgggacgg ctccaacccc aaccagttcc agctctacca gaaccttacc ggcgcgcctt
     2701 ccttcaacgg cgagtatatg caggctacct cacagcccgc acctggcagc accgtgcaag
     2761 accctgcgct ctactggctc tacccttaca acgggcagaa tcgtggcacc atcgataccg
     2821 ctcgctacca ctacatgacg gtcaagctgt ggctccaggc ccccgcggag cgacccggct
     2881 cgccgggcaa cctcggcccc cgcttcctgt ggtcgcaggg tggggactgg gtgcagaccg
     2941 agggcatgta cgcgccttac aaccgctgga tacctatgtc tgtggacctg gcgagcgtgc
     3001 cgaaagcccc cggctcgcct gcttacggct ggtcggggcc agtgaacatc ttccggttcg
     3061 acccgcacga agaggacgac accaggcaac cggcggtcaa cttgcctttc ttccgcatca
     3121 tgagcgcgca cctgatgtcc cagccgattg ccaacaacgc cacgctggtg cgctggggca
     3181 aggtgcaggg caccggctcg gtcaacctgt actacgacac caacaatagc gggttcgacg
     3241 gcacgctatt cgcatccaac gtaccgatag accaggggtt ggcgggctgg aacacgagcg
     3301 ggctgccgaa cggcaccgac tactgggtgt acgcggaagc cagcgatggg accaacacca
     3361 gccgctacta cagtctgctg ccggtgcgca tcgaccacgg ctcggccagc acgatcttcg
     3421 tggacgtgcc gaccaacaac ccgttcgcga acgacatcgc caacctggcg gctcgcaaca
     3481 tcatcaacgg ctacgggcag cccgacggca cgatgcactt caggccgggc gctacggcca
     3541 ccagggcgca actgagcaag atggtggtgc ttggcgcggg cggcacgctg gtcaaccccg
     3601 gcacgccgag cttcatggac gtgcctacaa gctcccccct ctacaccttc gtggagacgg
     3661 cggcggccag gggcatcatc agcggctaca cctgtggcac gaggccctct gagccttgcg
     3721 acggcagcgg gcgcaactac ttccgcccca acagggacgt tacccgcggg cagatctcga
     3781 agatgctgtc gcaggcgata ggccccttca cgggcacgag cgcacccctc aacaagtagc
     3841 ggcttccggg ctaaaggtac ttagaaagag acggaaccgc caagacgcca aggacgccaa
     3901 gagcgcgcca agaaataaga taagggtgtt ctaaacaaag agaaccaggg tacaccttcg
     3961 gtaccctggt tctctttgca ccacctaaac catcacaatc tctgcgcatc ctcgtgctac
     4021 aatctccggg attactcgtg tttgggatgc acgttggtat aat
//
LOCUS       DASRWZ010000438         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_653479, whole genome shotgun sequence.
ACCESSION   DASRWZ010000438 DASRWZ010000000
VERSION     DASRWZ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653479"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..524)
                     /locus_tag="VFH60_06845"
     CDS             complement(162..524)
                     /locus_tag="VFH60_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6313535.1"
                     /translation="MPHRGPILVIDDEPEILQTVATILEFEGYEVERAANGREGLDCI
                     ERSRPSLVLLDMRMPVLNGWDFARILKEKGVQLPIVVMTAAQDARRWAHEVGASGYIA
                     KPFDVDDLLTTVGSLVGA"
     gene            complement(493..>2383)
                     /locus_tag="VFH60_06850"
     CDS             complement(493..>2383)
                     /locus_tag="VFH60_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6313536.1"
                     /translation="LLFSATTIIIALSVANRLRLLADAPFLATAASSTSLLLLLALPY
                     LLLRLVDDFSNVPIWLMRASEVALGLMVLTVVVTTVVLGALPPLWLLAAMILYMVGFL
                     VYSAVAFWRESKRSSGVTKRRLAAVACGSIFLCALFVLVVLSISLPAFALAWRLLSDV
                     ASLASGISYFIGFAPPLFLRRAWQEPELRAFLGRAASLPRLPDTNSILRELERGTRSS
                     VGAPHASIGLWDQERQVLHFGTPATAMDIVPNHKSIAGRAFLTQKPALTDNIEIDNPT
                     GAKLALQYGARALLAAPITAGDRRLGVLVAYAPRAPIFADEDLELVKLLADQAAVVLE
                     SRALIDEAARVQAREEVARLKEDFLSAAAHDLKTPLTVLVAQAQFLERRVTRNPELPA
                     DPKSIQRIVQETQRLRELVLELLDASRAEQGMLVGEREEVDLMALAEQVCARRATERH
                     PCPVVAEGRVVGHYDEVRITQLIENLVENAIKYSPEGGAVLVKVWRGAGGNCLSVTDE
                     GIGIPSADIPRLFERFHRGTNVNDRKFSGMGLGLFICRGIAEQHGGRIWATSEPGKGS
                     TFQVMLPVNPPFSDPATGNQSDDAAREPSAARATATDIGSGSQTPLVLAEGGKNAASR
                     PNPGH"
BASE COUNT          414 a          757 c          769 g          443 t
ORIGIN      
        1 ggagagtaca aagaggacct gggtacatct gtcgtaccct ggtccttcta cttagaacgc
       61 catccataat cttggcgcgc tcttggcgtt cttggcgttc ttggcgttct tggcgtccct
      121 tcggctacgc tcagggcatg cttggcggtt ccgtctcttt attaggctcc cacgagagaa
      181 cctacggtgg tgagaaggtc gtccacgtcg aagggtttgg cgatgtagcc gctggccccg
      241 acttcgtgcg cccagcggcg ggcgtcctgc gcggccgtca tgaccactat gggcagttgc
      301 acgcctttct ccttcaggat gcgcgcgaag tcccagccgt tgagcacggg catgcgcatg
      361 tccaggagca cgaggctcgg gcgcgaccgc tctatgcagt ccaggccctc acggccgttg
      421 gctgcccgct ccacctcgta gccctcgaac tccaggatcg tagctactgt ttgcaggatc
      481 tccggctcgt cgtcaatgac caggattggg ccgcgatgcg gcattctttc ctccctccgc
      541 cagcacgaga ggcgtttgtg aacccgaacc aatatccgta gctgtggccc gcgccgcgga
      601 cggctcccgc gccgcgtcgt cgctctggtt ccccgttgct ggatcgctga atggagggtt
      661 aacggggagc atgacctgga aggtgctgcc tttgcccggc tcgctcgtcg cccagatgcg
      721 cccgccgtgt tgctcggcta tgccccggca gatgaacagg cccaggccca taccggaaaa
      781 cttgcggtcg tttacgttcg tcccgcggtg gaaccgctcg aacaggcgtg gtatgtcggc
      841 gcttggtatg ccgatgccct cgtccgtgac cgagaggcaa ttaccaccgg ctccgcgcca
      901 cacctttacg agcaccgcgc ccccttccgg gctgtacttg atggcgtttt ccaccaggtt
      961 ctcgattagc tgcgtgatac gcacctcgtc atagtgcccc acgacccggc cctcggccac
     1021 caccgggcag gggtggcgct cagtggcgcg gcgggcgcag acttgctcgg ccagggccat
     1081 gaggtctacc tcctcacgct ctcccaccag catgccttgt tccgccctgg aagcgtccag
     1141 caattcgagc accagctctc tcaaacgctg cgtctcctgc acgatgcgct gtatgctctt
     1201 ggggtcggcg ggcagctcgg ggttgcgggt gacgcggcgc tccaggaact gggcctgtgc
     1261 cacgagcacg gtgaggggcg tcttgaggtc gtgggcggcg gcggagagga agtcctcctt
     1321 cagccgggcc acctcctctc gcgcctgcac gcgcgcggcc tcgtctatga gcgcccggct
     1381 ctccagcacg acggccgcct ggtctgcaag cagcttcact agctcaaggt cctcatcggc
     1441 gaagataggg gcgcgtggcg cgtaggccac gagcacgccc aatcgccggt cacccgccgt
     1501 gatgggggcc gccagcaggg cacgcgcccc atactggagg gctaatttgg cacccgtggg
     1561 attgtctatt tcgatgttgt ccgtcagggc gggtttctgg gtgaggaaag ccctacccgc
     1621 tatagatttg tggttgggga ctatgtccat cgcggtagcg ggtgtcccga agtgtagtac
     1681 ctgacgttcc tgatcccaga ggccgatact ggcgtggggg gcacccacgg agctacgcgt
     1741 gccccgctca agctcgcgca ggatggagtt ggtatcgggc aggcggggca ggctggcggc
     1801 cctgcccagg aaggcccgca actctggctc ttgccaggcc cggcgcagga acaatggcgg
     1861 ggcgaagcca atgaaataac tgataccgga agccagtgaa gccacgtcgc tgagcagcct
     1921 ccacgcaagg gcaaaagccg gtagcgagat gctcaagacc acaagcacga agagggcgca
     1981 gaggaatatc gagccacagg caaccgcagc cagccgccgc ttcgtgacgc cgcttgaccg
     2041 cttcgattcg cgccagaacg ccacggcgct gtagaccagg aaccccacca tgtacaagat
     2101 catcgcggcg agtagccaga gcggtggcag tgctcccaaa acgaccgtgg tgacgactac
     2161 ggtcaggacc atcaggccga gggccacttc cgaggcgcgc atgagccata ttggcacatt
     2221 ggagaagtcg tctacgagcc tcagcagcag gtatggtagg gccagcagca acaggaggct
     2281 ggtggacgac gcggctgtag ccaggaaagg ggcatcggcc agcaggcgca gcctgtttgc
     2341 tacgcttagc gctatgatta tggtggtggc gctgaacagc agc
//
LOCUS       DASRWZ010000439         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_310958, whole genome shotgun sequence.
ACCESSION   DASRWZ010000439 DASRWZ010000000
VERSION     DASRWZ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310958"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..638)
                     /locus_tag="VFH60_06855"
     CDS             complement(123..638)
                     /locus_tag="VFH60_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313537.1"
                     /translation="MDEIYTGTPVAGARSAEEAAWDEEFNNTKGRSGVGSTLGEVAKL
                     TVQVPGAVASMAASMFPEDTRRHARAAVREGFLAVRSLLGAIGDSIENALSDKDAPAS
                     TRQPLSTSGPAGTWGTAPSAASPTPTGARARRIEISDLDSTATPAPTTSIGDDEDDAP
                     DERGLRAEIDY"
     gene            complement(743..1561)
                     /locus_tag="VFH60_06860"
     CDS             complement(743..1561)
                     /locus_tag="VFH60_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HET6313538.1"
                     /translation="MFFLISYLLAAAIGYLGYRARALSLSGAVAACVVGGTVFGFGGL
                     GAAALLVAFFASSSLLSFFKAGDPAKRRAAETFDKGGRRDAAQVVANGGVAALVALLG
                     YFFPSPLLAGAFLGSLAAATADTWATEVGVLSSKRPRLITTGAVVEVGTSGGVTLVGS
                     VSAAAGALFIGIVAALFSLLPVGSGLAVWAFSAIASALAGGIAGAFADSLLGATVQAS
                     FYCPACAKPTESRVHRCGTVSTPVGGVAWANNDLVNLAATAVGGAVGGTIAIVF"
     gene            complement(1674..2762)
                     /locus_tag="VFH60_06865"
     CDS             complement(1674..2762)
                     /locus_tag="VFH60_06865"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET6313539.1"
                     /translation="MPEELEPQAQKSHKQASGARSAQAGRDPRRQYIRLHSDENPYGC
                     SLRVQEILGSSDQYYLPADPMSNDLRAGISEYTGFAPERIVAGAGTVELTERMLHAFL
                     DAGDAVIVCPPALPQHNLGASRARVTLVQVPRDERFEVDTDAIVTTMRRQSNIKMVVL
                     SSPNNPTGNVTAPTAVVRLLQMGVWVLVDESYFEFADRTIAPLVAEFDNLVVVRSFSP
                     WAGLHGLPVGYALCSPRAASRLQRLTPPGALNRAAQLAAIASLGDVQALQQRVRRIRL
                     ERGRMYRQLRKLNLLQPYSSAAPFLLCRMTRGTSQLVQRHLQDDGILVKGISDRWLAN
                     HLRIGIGLPEDTNRLMTSLKALAGNPEL"
     gene            2918..>3385
                     /locus_tag="VFH60_06870"
     CDS             2918..>3385
                     /locus_tag="VFH60_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313540.1"
                     /translation="MEEYTSVPSGPISHSVELYTARLFIQGAISGPFKRTSDLINRRD
                     RDFFAVNGATVTALGQATNAPQKIAAPVQVGRDHIHFVATIPQQPSQPQAPGQTQTLS
                     PGREYYVHKQPIACYALTDTFIIHGQCHLLSGATLDTLLQSHDPFIPLTKATIY"
BASE COUNT          617 a         1237 c          933 g          598 t
ORIGIN      
        1 ccctggtcct cttgctttga acaccatcct ctaccttggc gttctttgcg tccttggcgt
       61 cttggcggtt ccgtctaatg agtcactcag accccgaagc tgccaacggt ggatgctatc
      121 ggctagtagt ctatctcggc ccgcagtcct ctctcgtcgg gggcgtcgtc ttcatcgtcg
      181 cctatcgagg tggtaggcgc gggtgtggcc gtggaatcga ggtcggatat ctcgatcctg
      241 cgcgccctgg caccggtggg ggtggggctt gcggcggagg gcgcggtgcc ccacgtaccc
      301 gcaggcccgc tcgtgctgag gggctgccgc gtgctggcgg gcgcatcctt atccgacagg
      361 gcgttctcga tactgtcccc gatggccccc agcaaagagc gcaccgccag gaagccctcc
      421 cgtaccgccg ccctggcgtg cctgcgcgtg tcctcgggga acatcgaagc cgccatcgac
      481 gccaccgcgc ccggcacctg tactgtcagc ttcgccactt cacccagcgt gctgcctacg
      541 ccgctgcgcc ccttcgtgtt gttgaactcc tcgtcccacg ctgcctcttc agccgagcgt
      601 gcacctgcca ccggagtacc cgtataaatc tcatccatag cgttcgctcc tctactcagc
      661 tttcacggcc tgccctaaca tgagtgcgtc cgaatattgc gtattataac accagccctg
      721 accctgtgta ccacgttccg cgtcagaaaa cgatcgcgat tgtcccaccc actgccccac
      781 ctaccgccgt agccgccagg ttcaccaggt cgttgttggc ccacgccaca cccccaaccg
      841 gcgtgctgac agtaccgcac ctgtggaccc tgctctccgt cggcttggcg cacgccggac
      901 agtaaaaact cgcctgtacc gtcgctccca gcaagctatc ggcaaacgcg ccggctatgc
      961 ctcccgccag cgcgctcgcg atggcagaga acgcccacac tgcaaggccg ctgcctaccg
     1021 ggagcagcga gaacaacgca gccactatgc ctatgaacag cgccccggcc gccgccgaca
     1081 cactgcccac cagcgtgacg cctcccgacg tacctacctc caccactgcc cccgtcgtga
     1141 tcaacctggg cctcttgcta ctgagcactc ccacctccgt agcccacgta tcggcggtag
     1201 ctgccgccag cgaccccagg aacgcgccag ccagcagggg cgacgggaag aaataaccaa
     1261 gtaaagccac cagcgcggcc accccgccgt tggccaccac ctgcgccgcg tctcgcctcc
     1321 cacccttgtc gaacgtctcc gctgccctac gcttggccgg gtcgcctgcc ttgaagaagg
     1381 aaagcaggct ggatgacgca aagaaagcca ccagcagcgc cgccgccccc aacccgccaa
     1441 acccgaacac cgtgcccccc accacacaag ccgccaccgc acccgaaagc gacagcgcac
     1501 gcgccctgta ccccaggtac ccaatagcag cagccaggag ataagaaatg aggaaaaaca
     1561 tgcggaattt ggaatttcgg atttggaatt tcgaatttag agcattctag cttactgagg
     1621 cgaacccact cggataccaa ctaaattcga aatccgaaat tcgcaattcc aaatcaaagc
     1681 tccgggtttc ccgccagcgc cttgaggctt gtcatgagcc tgttggtgtc ttcggggagg
     1741 ccgatgccta tgcgcaggtg gttggcgagc cagcggtctg atatgccctt gacgaggatg
     1801 ccgtcgtcct ggaggtgccg ctgcaccagt tgggaggtgc cgcgcgtcat ccggcacagc
     1861 aggaacggcg cggccgagga gtagggctgc aacaggttca gcttgcgaag ctgccggtac
     1921 atgcgccccc gctccaggcg tatccggcgc acccgctgtt gcagggcctg cacgtcccct
     1981 agggaggcta tcgcggccaa ctgcgcggcc cggttcaagg cccccggcgg cgtgagcctc
     2041 tgcaagcggg acgctgccct gggagagcac agggcgtagc ccaccggcag gccgtgcagc
     2101 cccgcccacg ggctgaacga gcgcaccacc accaggttgt cgaactcggc cacgagcggt
     2161 gcaatcgtgc ggtccgcgaa ctcgaagtag ctctcgtcca ccagcaccca cacccccatt
     2221 tgcagcagcc tcactacagc cgtcggggcc gtgacgttgc ccgtggggtt gttgggcgac
     2281 gacagcacca ccatcttgat gttcgactgc ctgcgcatgg tcgtcacgat agcgtcggtg
     2341 tccacctcga accgctcgtc tctcggcacc tgcaccagcg taaccctagc tcgcgacgcc
     2401 cccaggttgt gctgcggcag cgcggggggg cacacaatca ccgcgtcccc cgcgtcgagg
     2461 aaggcgtgca gcatgcgctc ggtaagctcc accgtgcccg cccccgccac tatccgctcc
     2521 ggcgcgaagc ccgtgtactc gcttatgccc gcccgcaggt cgttggacat cggatcggcg
     2581 ggcaggtagt actggtcgga cgagccgagt atctcctgca cccgcaacga gcagccatag
     2641 gggttctcgt cgctgtgcag ccggatgtac tgccgcctgg ggtcgcggcc cgcctgggcg
     2701 gacctcgctc cgctagcctg cttatgggac ttttgggcct gtggctctaa ctcttctggc
     2761 atctagtctc tgtttctgtg gcgtggtaag attattgaca ggtccgggac aatatctgtc
     2821 ctggcccctg ttatagcatg ggcgcacaag gagtatagca ttataaccaa tgtcgcccgg
     2881 cattgcacat attgaaccta tctcacggaa ggtaagcatg gaagagtata cgtccgtgcc
     2941 gtccgggcca atcagccaca gcgtcgagtt gtataccgcg cgcctcttca tccagggtgc
     3001 tatctccggc ccgttcaagc gcaccagcga cctcataaac cgccgcgacc gggacttctt
     3061 cgccgtgaac ggggccaccg ttaccgccct gggccaggct accaacgccc cccagaaaat
     3121 cgccgccccc gtgcaggtcg gccgcgacca catccacttc gtagccacca ttccccagca
     3181 gccctcccag ccgcaggccc caggccagac gcagaccctc agtcccggcc gcgagtacta
     3241 cgtccacaag caacccatcg cctgctacgc cctcaccgac accttcatca tccacggcca
     3301 gtgccacctg ctctcagggg ccaccctcga taccctcctc cagtcccacg accccttcat
     3361 ccccctcacc aaagccacca tctac
//
LOCUS       DASRWZ010000440         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_927800, whole genome shotgun sequence.
ACCESSION   DASRWZ010000440 DASRWZ010000000
VERSION     DASRWZ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927800"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..2823
                     /locus_tag="VFH60_06875"
     CDS             283..2823
                     /locus_tag="VFH60_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6313541.1"
                     /translation="MLASRGPSFGDWLKQGRKERDLTQLDLADHIGCSVATIQKIEAG
                     LRRPSRQVAQLLADFFHVPEDDRAAFVQFARGTSVASDRDEPDFDAPLDTAGVEQVVR
                     QNPAAEPAPRKAASNLSAPITSIVGREEEIARLSERLKWERVRLLTLTGPPGIGKTRL
                     AIEVGIGALDRFEDGVFFVPLAAVRDPGLVAPAIAEALGMRDTGFQPTNESLRAYLHD
                     KRMLLVLYNFEQVVEAAPAVVGLLQAAPGLKVLVTSRAALNVLGEQQFPIAPLPLPEL
                     TSLPSPEALLDYPSIRLFVERAQACNPAFELTHDNAPAVAAICARLDGLPLAIELVAA
                     RIKLLSPQALLPRLSNSLSFVKGGHKDLPARQQTLQDAIAWSYDLLEEGEKVLFARLS
                     VFSGGCTLNAIEAVCNAHNDLALDTLEGVQSLSDKSLLVQGLGRDEEPRFSMLVTIHE
                     YASEQLRQRGEVDELRDLHLEYYLAMTQAAEQQVQGNQQTIWFARLKSEHENLRAAIE
                     RTFERQQWERAAVLASRLEFFWRQGYPSEGRRWLEGLLPHAEAIPPWSLGRVYVALGQ
                     FAIDQGDLKQSDEWLGRALEIGRSIGDRELEARALDQTAWGAMIRGDFDRGNEYGRQA
                     LAIYEELGYKHRIVKTLYILGGTAAYGNRLEEASALFSRVVELARELGQVESLAGGLN
                     ALGVTEHFLGRHEAAMSHYNECLTLAREVGLPIHLAPVTGNIGKLFRKLGDYEQSRKY
                     LTDGMVLNHSLGLKDNIAETLIDFAELASAEGQHERAARLYGAGELMLQELGMPFPSL
                     AAGEMQAYIAETRQALGEERYQELWREGSKLSGEKAVEYALGKGEDGR"
BASE COUNT          640 a          944 c         1109 g          548 t
ORIGIN      
        1 tagtctccga aggagacttc gtctctggta agcccgccaa tttattggca gggcaccccc
       61 acagggctat ctcagggcga ccgaccgcct ttccaccgag aatgactaca ccctgtgcaa
      121 tccgcaagtg gggctgtgcc actgtatggc tctggtataa tatctatata gaattagaca
      181 acgcacgtaa ggcccgccag ggaagccggg ccttttccat accatattac tcacaattcg
      241 aaatccgaaa tccgcaattc gaaattgaca aggagtgggt tgttgctcgc cagcagggga
      301 ccatcattcg gggactggct caagcagggg cgcaaggaac gcgaccttac gcagctggac
      361 cttgccgacc atatcggctg ctcggtggct acgatacaga agatcgaagc cgggctgcgc
      421 cgtccctcca ggcaggtggc ccagcttttg gccgacttct tccacgtgcc cgaagacgac
      481 cgcgcggcct tcgtgcaatt cgccaggggc acctcggttg cgagcgaccg tgacgagcct
      541 gacttcgacg cgccgctgga caccgctggc gtggagcagg tcgtgcggca gaatcccgcc
      601 gccgagcctg cgcccaggaa ggcggcgagc aacctgtcgg cccccattac ctcgatagtg
      661 gggcgggagg aggaaatcgc gcggctgtcg gagcggctga agtgggagcg cgtgcgcctg
      721 ctcaccctca ccggccctcc cggcatcggc aagacccgcc tggctatcga agtcggtatc
      781 ggcgcgctcg accgcttcga ggatggcgtc ttcttcgtgc cactcgcggc ggtgcgtgac
      841 cccggcctgg tggcgcccgc catagccgag gcgctgggca tgagggacac ggggtttcag
      901 ccgaccaatg aaagcctgcg cgcgtacctt cacgacaagc gcatgctgct cgtgctgtac
      961 aacttcgagc aggtggtcga ggccgcgccc gctgttgtgg gcctgttgca agccgcgccc
     1021 ggcctgaagg tgctcgtgac cagccgcgcc gcgctcaacg tgctgggaga gcagcagttc
     1081 ccaatcgcgc cgctgcctct gccggagctt acctccctgc cttcacctga agccctgctg
     1141 gactacccct ctatccgcct gttcgtggag agggcgcagg cctgcaaccc cgcgttcgag
     1201 cttacgcacg acaacgcccc cgccgtggcc gccatttgcg cccgcctcga cggcctgccg
     1261 ctggctatag agctggtggc cgcccgcatc aagctgctgt cgccccaggc gttgctgccg
     1321 aggttgagca acagcctcag cttcgtgaag ggggggcata aagacctgcc cgcgcgccag
     1381 cagaccctac aggacgctat agcgtggagc tacgacctgc tggaagaggg ggagaaggtg
     1441 ctgttcgccc gcctgagcgt cttctccggc ggctgcacgc tcaacgcgat tgaggcggtg
     1501 tgcaacgcgc acaatgacct ggcgttggac acgctggaag gggtgcagtc gctcagcgac
     1561 aagagcctgc tggtgcaggg gctggggcga gacgaggagc cgcgcttctc gatgctggtg
     1621 acgatacacg agtacgcgtc ggagcagcta aggcagcgcg gggaggtgga tgagctgcgc
     1681 gacctgcacc tggagtatta cctcgcgatg acgcaggccg ccgagcagca ggtgcagggc
     1741 aaccagcaaa cgatatggtt cgcccggctc aagtcggagc acgaaaacct gagggccgcg
     1801 atagagcgca ctttcgagcg gcagcagtgg gagagggccg ccgtgcttgc gagccgcctt
     1861 gagttcttct ggcggcaggg ctacccgagc gaggggcggc gctggctgga gggactgctg
     1921 ccacacgctg aggctatccc gccgtggagc ctggggaggg tatacgtggc gctgggccag
     1981 ttcgctatcg accagggcga cctgaagcaa tcggacgagt ggcttggacg ggcgctggag
     2041 attggcagat cgattggaga cagggagcta gaagcccgcg cgctggacca aacggcctgg
     2101 ggcgcgatga tacgcggcga tttcgaccgt gggaacgagt acggcaggca ggccctcgcc
     2161 atctacgagg aattggggta caagcaccgg atagtgaaaa cgctatatat cctggggggc
     2221 acggcggcat acgggaacag gctggaggag gcaagcgcgc tcttctcgcg agtggtagag
     2281 ctggcgcgcg agctggggca ggttgagagc ctggccgggg gcctcaacgc gctaggggtg
     2341 acggagcact tcctgggcag gcacgaggcc gctatgtcgc attacaacga gtgtctcacg
     2401 ctggcgcggg aggttgggct gcctatacac ctcgcgcctg ttacggggaa cataggcaag
     2461 ctcttccgca agctgggcga ctacgagcag tcgaggaagt acctcacgga tggcatggtg
     2521 ctcaaccata gcctggggct gaaggacaac atcgcggaga cgctgattga cttcgcggag
     2581 cttgctagcg cggaggggca acacgagcgg gcggcgcgcc tgtatggggc gggagagctg
     2641 atgctgcaag agcttggcat gcccttcccc tcgttggcgg caggcgaaat gcaggcgtac
     2701 atagccgaga cccgccaggc gctgggggag gaacgctacc aggagctgtg gagggaagga
     2761 agcaagctgt ccggcgagaa ggcggtggag tacgcgcttg ggaagggaga agatgggcga
     2821 tagaggtaag agagacgcgc tgccgaggcc gccccaccgt agcccagtgg gggacgccct
     2881 gtggctaaag ccacgggctg agaagaggca aagtctccga aggagactga ggagtatctg
     2941 caaaccgtaa ggaataaccg accaagggtg gaccttgtca ttctgagcgt agcgaagaat
     3001 ctcgtcctcc atgcaggtat tgcccttttc atcagcacat gggacgctac cgggcgaaga
     3061 aaaggggtat ttgggtgctg gaggacgaga ttcttcgctg cgctcagaat gacaaaggcg
     3121 gagcttggtc ccgcatgcgc agcactttgc ggataggctc taccttcgcg tttcctagtc
     3181 tccgaaggaa acttcgctac ttctcagacc gtggcttatg aagattaaca actgaatcag
     3241 g
//
LOCUS       DASRWZ010000441         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_174103, whole genome shotgun sequence.
ACCESSION   DASRWZ010000441 DASRWZ010000000
VERSION     DASRWZ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174103"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(469..1446)
                     /locus_tag="VFH60_06880"
     CDS             complement(469..1446)
                     /locus_tag="VFH60_06880"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET6313542.1"
                     /translation="MNNLEHLSIFSGRANPDLAEATARNLNVDLGNIDVFEFSNENIF
                     VKIDESVRENDVFIIQSLTSPVNTSIMELLIIIDAAKRASAHRITAVIPYYAYGRSDK
                     KDQPRVPITSRLIADLITVAGADRVLTIDLHAGQIQGFFNIPVDELTTMYLLADHFIK
                     KRIPDRVVVSTDIGNAKRARNFAELLDAPLAIVEKRRSGNSGGTEVLNLIGEVEGMNA
                     ILVDDEIDTGGSIIQAANMVKEGGARTIYACCAHAVLSGPAIERLRNSPIRELVTTDT
                     VPLPPEKRLPSITVLSVAPMLAETIARIHTGRSVGAMFQADKHLILDNN"
     gene            complement(1446..>2336)
                     /gene="rnc"
                     /locus_tag="VFH60_06885"
     CDS             complement(1446..>2336)
                     /gene="rnc"
                     /locus_tag="VFH60_06885"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET6313543.1"
                     /translation="AHDEPQAAEEPPQEDGLDTGGQGEVGGDEARGEGALPQRPLDEL
                     EEKIGYRFEDRQHLVNALVHKSYLHAVPDFPPGSNERLEFLGDSVLGFIVSSDLYTEQ
                     PGMSEGQLSALRGVLVRLTTLAQLAGPLEIGQYMYMSRGEEAAGGRARGSNMGRAIEA
                     ILGAAYLDGGLEAASRVWHTILGVRSMEQLQAVLRSDYKSALQQFTQAHLKATPEYRI
                     VGTSGPEHAKQFHVEVRAGGRVLAGGVGRNKQIAEQSAAEAALSSLQAEMPVGDTVEQ
                     SASETVVIDNGQGRGEEAKA"
BASE COUNT          396 a          708 c          739 g          493 t
ORIGIN      
        1 tgcgcgttgt tttggcgctg aattcttatc tggcgctgaa gaagcacgcc taaataaaca
       61 ttgaactttt ctactgtcaa gtgaattata gcagccttag cactggggat ttaatttcca
      121 cacaatcata gcactagacc cgagcattag ttgactgctc gctaaaagaa gtgttagcca
      181 cctggagagg cactggttgt gctggtgcaa tccgcagatg cgcggtgaca aggggtactc
      241 agggtccagc aaagaggggg aagtgttgct gccacaacgc tcacgagccg tgcggatatg
      301 ctctataagc ctggactgac ctgagtaatt tgatgctcgt ctgcaatcag gctataggcg
      361 ggcaactaag aaaatacgca agacttactg gctgcgagtc aagtcttaac ttagctcggt
      421 gccggcacgc cgcatagcct tcagggtgaa tttctgaccg aagggtctct agttgttgtc
      481 caaaatgagg tgcttgtcgg cctggaacat ggcacccacg gagcggccgg tgtggatgcg
      541 ggcgatagtc tcggccagca tgggggccac gctcaggacc gtgatgctgg ggaggcgctt
      601 ttcaggtggg aggggcacgg tgtcggtggt caccagctcg cggatggggc tgtttcgcag
      661 gcgctctata gcggggccgg agagcacggc gtgggcgcag caggcgtaga tcgtcctggc
      721 cccgccctcc ttgaccatgt tggcggcctg gataatagag ccgcctgtgt cgatttcgtc
      781 gtccaccagg atggcgttca tgccctcgac ctcgccgatg aggttgagca cctcggtgcc
      841 gccgctgttg ccgctccgcc gtttctccac gattgccagg ggcgcgtcta aaagctccgc
      901 gaagttgcgc gcgcgcttgg cgttgcctat atcggtggac accaccaccc ggtcgggaat
      961 gcgcttcttg atgaagtggt cggccagcag gtacatcgtg gtcaactcgt ccacagggat
     1021 gttgaagaag ccctggattt gccccgcgtg caggtcgatt gtgagtaccc tgtcggcccc
     1081 ggcgaccgtt atcaggtccg cgatgagccg agaagtgatg ggcacgcggg gctggtcctt
     1141 cttgtcgctg cgcccgtaag cgtagtaggg gatgacggcg gtgatgcggt gggccgaggc
     1201 tcgcttggcg gcgtcaatga tgatgagcaa ctccatgatg gaggtgttca ccgggctggt
     1261 gagggactgg atgatgaaga cgtcgttctc ccgcacgctc tcgtctatct tcacgaagat
     1321 gttttcgttg gagaactcga acacgtctat attacccagg tccacgttga ggttgcgcgc
     1381 ggtggcctcg gcaagatcgg ggttggccct gccggagaaa atcgacaggt gctccaggtt
     1441 gttcatcaag cctttgcttc ctcccccctg ccctggccgt tatcgatgac taccgtctcc
     1501 gacgcgctct gttctaccgt gtcgcctacc ggcatctcag cttgcaagct tgacagcgcg
     1561 gcctcggcgg cggactgctc ggcaatctgc ttgttgcggc ccaccccgcc cgcgagcact
     1621 ctgcccccgg cgcgcacctc cacgtggaac tgcttggcgt gctccggccc cgacgtgccg
     1681 acgatccggt actcgggtgt ggccttgagg tgggcctggg tgaactgctg tagcgcgctc
     1741 ttgtagtcgc tacgcagcac cgcttgaagc tgctccatgc tccgcacgcc caggattgtg
     1801 tgccacaccc gcgaggccgc ttccaggcca ccgtcgagat acgcagcacc caggatggcc
     1861 tcgatggccc ggcccatgtt gctgccccgc gcccggccgc ccgcggcttc ctccccgcgg
     1921 ctcatgtaca tgtattggcc gatttccagc ggccccgcaa gctgcgccag cgtggtcagg
     1981 cggaccagca cgccgcgcag cgccgaaagc tgcccctcgc tcatgccggg ctgctccgta
     2041 tacaggtcgc tcgacacaat gaagccgagc acggagtcgc ccaggaactc aaggcgctcg
     2101 ttcgagccgg gcgggaagtc gggcaccgcg tgcagatatg acttgtggac gagggcgttg
     2161 accaggtgct ggcggtcctc gaaccggtag ccgatcttct cctcaagctc gtccagcggc
     2221 ctctgtggta gcgcgccttc gccacgtgcc tcatctccac caacctcgcc ctgcccgccg
     2281 gtgtcgaggc cgtcctcctg gggtggctct tctgcggctt gcggctcgtc gtgcgc
//
LOCUS       DASRWZ010000442         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_105619, whole genome shotgun sequence.
ACCESSION   DASRWZ010000442 DASRWZ010000000
VERSION     DASRWZ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105619"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..>3343
                     /locus_tag="VFH60_06890"
     CDS             408..>3343
                     /locus_tag="VFH60_06890"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HET6313544.1"
                     /translation="MSFLRRFLANKTARVALALCFLTGAALQAIPLNLFPSTAVAEVS
                     AQAGHAPAVEGWVKDSAGSQPVAGAQVLLNDTLRASTDVQGYFSFDSEALGQFGTSGE
                     QGLPVSVQVSAPGHANWSISNARYFSGAALRLHASLRAANEGPSVFTAASPSGRSLEK
                     QIADGLTLARPGRSIQDQLTLDDASVQANAPASSSALVPPATIRVYRTAPGVVEVVPF
                     REYVKHVLPSEWIPTWKMASLQAGAMAVKSYGWYWVSQGGKQTALGADVKDNTEDQVY
                     DPNVSYSTTDEAVDSTYDYAMTRAGALFQAQYCAGSYDEDPSGECPWGGPYMTQWGSA
                     WHADQGRNWGWLLQFYYRDAVITPAPPGGGYDGPPVAPSTPAPRRPAPTAPAAAPANY
                     SVGQGADSPQVFQEAYDRNGGAAALGKPTGPVRWWLTDISEHNVVAQPFSGPDGKGNT
                     WLVFDVLKAPNMGVSRAFLLQGALATVYAQHEPPGPEWLGAPTSDPFTVDVGSGAVTR
                     QGFVRGSLRLSGGSVEAAPWPQSFPNWKAEYFAGHHVPSAQLGPQRSLPGYPAFVQDV
                     QTPSFSWSADQQMPYTLGAGKADWSVQFTRQEQTDGGTYEFTLSADSGVRLWIDNMLA
                     INGWHWTSPQQERYTADLSPGAHQVRVQYYNLDASAQLGFEMKKQGDPAPPPANVSAP
                     APQPQPQQPPQAQSTAQLRVRVQWIGRGAAPSDSWVQPLTLHLSNPATAGVVASYNGA
                     TDRNGVAHYTGLPAGIYNLHVKGAHSLQSARANIALTSGATLDIDMKMQVEGDVNGDN
                     CVTVDDFSYLQARLGTHRNAGGFDPAADLNNDGDVSVTDVSLLRSGFDRCGDVSADAQ
                     FGVQSASAAPPLSQLLAPWLRPETLARDLGLSIWTSADQVRVGEYLDVAIVAQAGAQP
                     IDAASFVMQFDPAVFSPVDAAGNPAAAMEPGLALPSVMGNWLDANGGAAGFAAGMLQG
                     ETPSGEV"
BASE COUNT          582 a         1139 c         1036 g          586 t
ORIGIN      
        1 cgcgggcgag tacctgcagg tttcgggcgc gggtccaacg cgcaagggag ttcacctgct
       61 gctccgcgtc gtatagaggc gcgtaccgtg taaacgcgcg tgcggtgcgg gtgccctgag
      121 tgtcgcgcgt ggcccgtggc cgagcggatt ttatcttttc cacgatgagc agtatatcaa
      181 atgcggagcg cgcagggtat taggaccttg ccccctctcc cccgcccttc cggcatgtgt
      241 aacgcaagcg tcccacatga cataagtaca tttagcctat tgtgctgaca taatgttttc
      301 ttttattatc gttctgagac ggcaacgtaa gctgttttcg accctgccgc tcaattgcac
      361 agccctattt cgatgaccct accacactgc tcaacaatgg agggccaatg tcgttcttgc
      421 gacgatttct ggcaaacaag accgctcgtg tcgcacttgc tctttgcttc ttgaccgggg
      481 ccgcgctcca ggctatccct ctgaacctct tcccttcgac ggccgtggcg gaagtctcag
      541 cccaggccgg gcatgccccc gcggttgaag gctgggtcaa agatagcgcc ggctcccagc
      601 ctgtagccgg tgcccaggtc ctcctgaacg acacgctacg ggcctccacg gacgtacaag
      661 ggtacttctc cttcgacagc gaagcactgg gccaatttgg tacctcaggc gagcaaggcc
      721 tgcctgtatc ggtccaggta agcgcgcccg gccatgcaaa ctggtcgatc tccaacgcca
      781 ggtactttag cggcgctgcc ctgcgtctgc atgccagcct ccgcgccgca aacgaggggc
      841 cttccgtttt taccgccgcc tccccctcgg gtcgaagcct cgagaagcag attgcggacg
      901 gcctgaccct cgctcgtccc ggccgctcca tacaggacca attgaccctg gacgatgcct
      961 cggtgcaggc aaacgccccc gcctcctcgt cagccctagt ccctcccgcc acaatccgcg
     1021 tgtaccgcac cgccccgggc gtggtggaag tcgtgccctt tcgggagtac gtaaagcacg
     1081 tcctgccctc ggagtggatc cccacctgga agatggcgtc tttgcaggcc ggcgcgatgg
     1141 ccgtgaagag ctatggctgg tactgggtgt cgcagggcgg caagcagacc gcgctgggtg
     1201 ctgacgtgaa ggacaacacg gaggaccagg tatacgaccc caacgtcagc tactcgacca
     1261 ccgacgaggc cgttgacagc acctacgatt acgctatgac ccgtgcgggt gcccttttcc
     1321 aggcgcagta ttgcgcaggc tcctacgacg aagaccccag cggagagtgt ccctggggcg
     1381 ggccttacat gactcagtgg ggaagcgcct ggcacgccga ccagggccgc aactggggct
     1441 ggctgttgca gttctactat cgcgacgccg tcatcacccc cgcgccgccg ggtggcggct
     1501 acgacggtcc tccggtggcc ccctccacac ccgcccccag gaggcccgca ccgacggctc
     1561 cggccgctgc tcccgccaat tacagcgttg gccagggagc ggacagcccg caggtcttcc
     1621 aggaggcgta cgaccgcaac ggcggcgcgg cggctcttgg caagcccacc ggccccgtgc
     1681 gctggtggct gactgatatt tccgagcaca acgtggtagc gcagcccttc agcggccccg
     1741 acggcaaggg caatacctgg ctcgtgttcg acgtgctgaa agcgcccaac atgggagtca
     1801 gcagggcgtt cctgctgcaa ggcgcacttg cgacggtata cgcccaacac gagcctcccg
     1861 gccccgaatg gctcggtgcg cctacctcag acccgttcac ggtcgatgtg ggtagcggcg
     1921 cggtcacgcg gcaggggttc gtgcggggca gcctccgctt gtctggtggc agcgtagagg
     1981 ccgcaccctg gccccagagc ttcccgaact ggaaagccga gtacttcgcc gggcaccacg
     2041 tgccctcagc ccagcttggc ccccagcgtt cgctgcccgg ctatcccgct ttcgtgcagg
     2101 acgtgcaaac gcccagcttc tcgtggtccg ccgaccagca gatgccctat acactcggcg
     2161 cgggcaaagc agactggtcg gtgcagttca cccgccagga acagacggac ggcggcacgt
     2221 acgagtttac gctgagcgcc gatagcggtg ttcgcctctg gattgacaac atgctcgcca
     2281 tcaacggctg gcactggacc tcgcctcagc aggaacgcta tacggcggac ctgtcgcccg
     2341 gcgcgcacca ggtccgggtg cagtattaca acctggatgc ctccgcccag cttggcttcg
     2401 agatgaagaa gcagggagac ccggcaccgc cccccgccaa cgtgtcggcc cctgcccctc
     2461 agcctcagcc tcagcagcct ccacaggcgc agagtacggc acagttgcgc gtgcgcgtgc
     2521 agtggatagg ccgtggcgct gcgccgagcg atagctgggt gcagccgctg acccttcacc
     2581 tgagcaaccc cgccactgca ggcgtcgtag cctcttataa tggtgcgacc gacagaaacg
     2641 gcgtggcgca ttacacgggc cttcctgcgg gcatatacaa cctgcatgtg aagggcgcgc
     2701 acagcctgca gagcgcccgt gccaatatcg cgcttacttc aggcgcgacc ctggacatcg
     2761 acatgaagat gcaggtcgaa ggggacgtga acggcgacaa ctgcgtgacg gtggacgatt
     2821 tctcatacct gcaagcccgc ctcggcaccc accgcaacgc cgggggcttc gaccccgcag
     2881 ccgacctgaa caacgacgga gacgtgagcg taaccgacgt gagcctcctg cgttccggtt
     2941 tcgaccgttg cggcgacgtg tcggccgatg cccagttcgg cgtccagagc gcgagcgcgg
     3001 cccctcccct ctcgcaactg ctggccccct ggctgcgacc tgagacgctg gcgcgggacc
     3061 tgggcctctc gatctggacc tccgccgacc aggtgcgcgt gggcgagtac ctggacgtgg
     3121 caatagtggc ccaggccggc gctcaaccca tcgatgcagc ctcgttcgtc atgcagttcg
     3181 accccgcggt gttctcaccg gtggacgcgg cgggcaaccc ggcagccgcg atggagcctg
     3241 ggttggcgct accttccgtc atgggcaatt ggctggacgc aaatggtggc gcggcgggct
     3301 tcgcggcagg catgctacag ggcgagacac cttcgggcga ggt
//
LOCUS       DASRWZ010000443         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1133443, whole genome shotgun sequence.
ACCESSION   DASRWZ010000443 DASRWZ010000000
VERSION     DASRWZ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133443"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2171
                     /locus_tag="VFH60_06895"
     CDS             <1..2171
                     /locus_tag="VFH60_06895"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET6313545.1"
                     /translation="LSLGLYSATLARTISYTSFDSPELVTKSGLLQMAHIPGAPVYVW
                     LGHLFTSLPFGEPATRVNWMSALLGALSVGILYAVISRHLTGDRLTAVGGSALFALSL
                     TFWSQAVIAELYAPNLCFLAFVVLALFEWGAARSDPARATGDAGRRWLVAAAAVFGLS
                     LGIHPSNVLYLPAFGAFALLGWPRQPRLGTVSGTPGEGGATAFLRRFDRRGGLLSLAV
                     GVVAVAVPYTWVYVDLPNVPYGDFFPRAEPGWPLFQAVTFNAFSDARFGYTLQEVPDR
                     IALFLHLLSRNMGPVGVPLLVLGAWRLLFSRLRPFFLLVPMMLANLVFYINYKVPDSD
                     VYYIPTYWVTACFAAFGLEAVAVAVQALLRVPSRLSATPAMNLAPGRRLGLQAGAWGI
                     TDLKSILPALVVATLTLGGAGWEWRNNVAPNNMSKDVSIRDFYGNALAMLPQGANLYH
                     RGASLGYDLLYYTRLCGVRPDLRVQAGVGANDPPPGAVPWPEGPVYSSIKGDDAWPPN
                     FIANPDGSNLKWYEPVLTGMFHSDVGMQYGWLNLYRVRPAGDPPLDWMVSIEDPASHP
                     SQKLNVDYTALLTLVGVDAEPQATPGKPWRLVRYWYARGMWLPPMVTVLGDHLAMEGH
                     VPLFGQFQQYVAAREIGDLGNFVVRDETYLVIPSNIAPGRYPVSIAMAKPRLLSLMLD
                     PARPEELMSNAAVVTTVEVVAPTEAPPDPLSQATNGACRP"
     gene            2168..2326
                     /locus_tag="VFH60_06900"
     CDS             2168..2326
                     /locus_tag="VFH60_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313546.1"
                     /translation="MNGSPVRRWLVGIAVICLLLLAAFGAGLYAGEQGWTTPTVPPWP
                     EMARDVGE"
     gene            2328..2489
                     /locus_tag="VFH60_06905"
     CDS             2328..2489
                     /locus_tag="VFH60_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313547.1"
                     /translation="MQEHGTNDHDFGSGATEPRPRWLVALGVLLLVAMMLAAAFALGV
                     YVAERNLFS"
BASE COUNT          410 a          922 c          872 g          534 t
ORIGIN      
        1 tgctcagcct gggcctttac agcgcgacgt tggcccgcac cattagctac accagcttcg
       61 actcgcccga gttggtcacc aagagcggcc tgctccagat ggcccacata cccggcgcgc
      121 ccgtgtacgt ctggctgggg cacctgttca cctcgctgcc cttcggtgag cctgcgaccc
      181 gcgtcaactg gatgtccgcg ctgctcggtg ccctttcggt gggcatcttg tacgcggtaa
      241 tctcgcgcca cctgacgggc gaccgcctaa ccgctgtggg gggcagcgcc ctcttcgccc
      301 tgtctctcac cttctggtcg caagccgtca tcgcggagct ttacgcgccc aacctctgct
      361 ttctggcctt cgtggtgctc gccctgttcg agtggggggc cgccaggagc gaccctgctc
      421 gcgccaccgg cgacgctgga cggcgttggc tcgtggccgc cgctgccgtg ttcgggctga
      481 gcctgggcat ccacccttcc aacgtgctct acctccccgc cttcggagcg ttcgccttgc
      541 tcggctggcc gagacaacca cgcctcggca cggtgagtgg gacgccaggc gaaggtggcg
      601 cgaccgcgtt ccttcgccgc ttcgaccggc ggggcgggct gctcagcctg gcggtgggtg
      661 ttgtggcggt agcggtgccc tacacgtggg tctacgttga cctgcctaac gtgccctacg
      721 gcgacttctt ccccagggcc gagccgggct ggcccctctt tcaggcggtg actttcaacg
      781 cgttcagcga cgcccgcttc ggctacacgc tccaggaggt gcccgaccgg atagccctct
      841 tcctgcacct gctgagccgc aacatggggc cggtaggggt gccgctcctg gtgctgggtg
      901 cgtggcggct gctcttctcc cggctgcggc ccttcttctt gctcgtgcct atgatgctgg
      961 cgaacctggt cttttacatc aactacaagg tgcccgacag cgacgtttac tacatcccta
     1021 cctactgggt gacggcctgt ttcgccgctt tcggcctgga agctgtcgcc gtggccgtgc
     1081 aggcgctcct gcgcgtgcct tccaggcttt cggcgactcc cgccatgaac cttgcgccag
     1141 gccgcaggtt gggactgcaa gcgggagcct ggggcatcac cgacctgaag tcgatactgc
     1201 ccgcgctcgt ggtggctacc ctgacgcttg ggggcgcggg ctgggagtgg cgcaacaacg
     1261 tggcacccaa caacatgtcg aaagacgtga gcatccgcga cttctacggc aatgcgctgg
     1321 cgatgctgcc gcagggggcc aacctgtacc accggggggc gtccctcggc tacgacctgc
     1381 tctactacac caggttgtgc ggcgtgcgtc cagacctgcg cgtgcaggcg ggcgtggggg
     1441 ccaacgaccc tccccccggc gcggtgccct ggcccgaagg cccggtctac tcgagcatca
     1501 agggcgacga tgcctggccg cctaacttca tcgcgaaccc cgatggcagc aacctcaagt
     1561 ggtacgagcc ggtgctgacg ggcatgttcc actcggacgt gggcatgcag tacggctggc
     1621 tgaacctgta tcgcgtgcgc cccgccggcg acccgccgct tgactggatg gtgtcgatag
     1681 aggaccccgc ttcccacccc tcgcagaagc tgaacgtgga ttatactgcg ctgctcacgc
     1741 tcgtgggcgt ggacgccgag ccgcaggcca cgccgggcaa accgtggcgg ctggtgcgat
     1801 actggtacgc caggggcatg tggttgcctc ctatggtaac cgtgctgggc gaccacctgg
     1861 cgatggaggg acacgtacct ctcttcggcc agttccagca gtacgtggcc gcccgcgaga
     1921 ttggcgacct gggcaacttc gtagtgcgcg acgagaccta cctcgttatc ccctcgaata
     1981 tcgcccccgg ccgctacccc gtaagcatcg cgatggctaa gccccggctg ctttccctca
     2041 tgctggaccc cgcccggccc gaggagctga tgagcaacgc agcggtggtc accacggtgg
     2101 aagtagtagc gcccacggaa gccccgcccg accctctctc gcaggccacc aacggagcgt
     2161 gcagaccatg aacggctccc cggtgcggcg gtggctggta ggcatagcgg tgatatgcct
     2221 gttgttgctg gccgctttcg gcgcgggcct ctacgcaggc gagcagggct ggaccactcc
     2281 cacggtgccg ccctggccgg aaatggcccg cgatgtggga gagtgatatg caggagcacg
     2341 gcaccaacga ccacgatttc gggtctgggg caactgagcc tcgccctcgc tggctggtgg
     2401 ctcttggtgt tctgttgctg gtggctatga tgcttgccgc agcgttcgcg ctgggggtgt
     2461 acgtagcgga gcgcaacctc ttcagctagg cgagagcaac atacaagtag gcgaaaatcc
     2521 ccagacgcca ggtggaatct gccctgccca tgggaatccc ttaagtgggc agggcagatt
     2581 cttgggtcta ttgccttcag gttccttcgt tcgaacgtgc cacgctctcc tatgtgtctc
     2641 gaacaattct taaggcctat ctgcaaagtg ccgcccatat gggaccagat tggcccttgt
     2701 cattctgagc gtagcgaaga atctcgtcct ccagcacc
//
LOCUS       DASRWZ010000444         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1064919, whole genome shotgun sequence.
ACCESSION   DASRWZ010000444 DASRWZ010000000
VERSION     DASRWZ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064919"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFH60_06910"
     CDS             complement(<1..309)
                     /locus_tag="VFH60_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313548.1"
                     /translation="MDVIWLLAAGAIMLLVGLSALAADGFRRRSNPFPRLMRDLTTTL
                     IRPGAALWGHSVHASGSLAPPTSGQWRPMKPRGLRALLGRITAPRIVPLVFFAGLGAT
                     C"
     gene            complement(312..1673)
                     /locus_tag="VFH60_06915"
     CDS             complement(312..1673)
                     /locus_tag="VFH60_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET6313549.1"
                     /translation="MDKRSKLEAPAPDSPQPEAPLAARMRPRTLDEIVGQEHVLAPGK
                     TLRRAIEADRVPSLVLWGPPGSGKTTLARVIANTTNAHFAPVSAVSAGVADLRRIVKE
                     AAERITGDEGRTILFIDEIHRFNKAQQDAILPHVEEGTVTLIGATTENPSFEVNSALL
                     SRSRVIVLHALDADDLDKLIERALSDERGLAHLNVTLEPDARQHLLNISDGDARSMLN
                     TLELAAVSTEPGPGGTRHVTLAGVEDAAQQRWLQYDRAGEGHYDAISALHKSVRDSDP
                     DGALYWLARMLEAGEDPLFVARRVIRMASEDIGMADPNALGMAMAAQQAVHFLGMPEA
                     NLALSQAVVYVALAPKSNALYAAYKLVQDDVMRTRNEPVPMHLRNAPTGLMKAVGYGQ
                     GYKYAHDYEGGQVEQSHLPDSLKGRRYYRPTERGYEARLRQRMQEWAEQRKRNASTEG
                     QKG"
     gene            2333..>5199
                     /locus_tag="VFH60_06920"
     CDS             2333..>5199
                     /locus_tag="VFH60_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017740246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6313550.1"
                     /translation="MPVSQTWTITSQISTNDNWSGVPGFMGYLGDDPTTTVAPVNPLS
                     IITESATIDVIANQNNTAITNGGVAEFEITDPVVALQGSNTADFPNIVIRVNTTGWQN
                     INVSYLIRDIDGTADDAAQAVNLQYRVGASGTFTNVTGGYIADATTGGTAVQTTPVSA
                     VLPAAADNQPIVDIRIITGNAIGSDEWVGIDDIAIFGTEIGTATNTAVPTETSTPEDT
                     VTGTPPTETNTAIPTDTPIPTNTSTATQTATASATAVTTLRIRDIQDSSHISPYNGTS
                     VSNIPGIVTGIITPPGNNNDGFYLQDPTSDANDSTSEAIFVHTGNAPTVSVGNSLLVG
                     GTVSEFRPGGSGSDNLTLTQIGGTLSITVISTTAVLPTPVVIGTGGRIPPTTIIEDDT
                     SGSVETSGAFDPAQDGIDFYESLEAMRVQVNNPVAVGPRNGFGEIAVLGDDGANASVR
                     TARGGIVIQPNDFNPERIILDDVITSTPAGVDVGDHFTGPAVGIMDYGFGNFKLYITS
                     PLTVVSGNLQQETAVPAVQGALSIGNYNVENLDPSDSTFGLHASAIITNLKAPDLLSI
                     EEIQDNSGSTNDGTVAADETWDELIKAIEDAGGPTYEYRQINPVNNQDGGEPGGNIRV
                     GLLFRSDRGLSFVDKPGGTSTNACTIQPDGTLTMSPCRVDPTNAAFNNSRKPLAGEFL
                     YNGETIIVIANHFSSKGGDDPLFGRWQPPIRSTEAARHQQAASVNGFVDSILNADAGA
                     NVVVAGDINDFQFSETMNILETGVISGTGDIELQNLIETLPLNEQYTYVFDGNSQALD
                     HALVSPNMFGYLVQYDVVHMNAEFDPQASDHDPSVGRFNMFGADTATPTLTAVPSNTS
                     TSTTVPSSTSTSTSTATRTSTTVPSSTSTTAATAVPSATATACTIEFADVPPTGEGST
                     FYEFIRCLACRGIVGGYPCGGPGEPCNENNDPYYRPGANVTR"
BASE COUNT          984 a         1594 c         1607 g         1014 t
ORIGIN      
        1 gcaggtggcc cccagtccgg cgaagaagac gaggggcacg atgcgtggcg cggttattct
       61 tcccaggagc gcgcgcaagc cacggggctt catgggccgc cactggccgg aggtgggcgg
      121 tgcgagggag ccggaggcgt ggacggagtg gccccacagg gcggcaccgg gccgtatcag
      181 ggtggtggtg aggtcgcgca tgagcctggg gaaggggttg ctgcgccgcc tgaagccgtc
      241 agccgcgagc gcggatagtc ctacgaggag catgatagcc ccggctgcca gcagccagat
      301 tacgtccacg cctagccctt ttgcccttcg gtcgaggcgt tacgcttgcg ctgctcggcc
      361 cactcctgca tccgctggcg aaggcgtgcc tcgtagccgc gctcagtagg gcggtagtag
      421 cgcctgccct tgagggagtc ggggaggtgg ctttgctcga cctggccgcc ttcgtagtcg
      481 tgggcgtatt tgtagccctg gccgtagcct accgctttca tcagcccggt gggggcattg
      541 cgcaggtgca tcggcacggg ctcgttgcgg gtgcgcatca cgtcgtcctg taccagcttg
      601 taggccgcgt agagggcgtt gctcttgggg gcgagcgcta cgtacaccac ggcctgcgac
      661 agcgcgaggt tggcctccgg catgccgagg aagtgcaccg cctgctgagc ggccatagcc
      721 atccctagtg cgttgggatc ggccatgcct atatcttcgg aggccatgcg gatcacgcgg
      781 cgggcgacga aaagcgggtc ctccccggct tccagcatgc gggcgagcca atagagcgcg
      841 ccgtcggggt cggaatcgcg caccgacttg tggagggcgg agatggcgtc gtagtggcct
      901 tcgcccgcgc ggtcgtattg cagccagcgt tgttgggcgg cgtcctccac cccggcgagg
      961 gtgacgtggc gcgtcccacc ggggccgggt tcggtagaga cggcggcgag ttccagtgtg
     1021 ttgagcatgg agcgggcgtc gccgtcggag atattgagca ggtgttggcg ggcgtccggc
     1081 tccagcgtga cgttgaggtg cgccagtccg cgctcgtcgg agagcgcccg ctctattagc
     1141 ttgtccaggt cgtccgcgtc gagggcgtgc agcacaatca cgcgggagcg agagaggagg
     1201 gccgagttca cctcgaagga ggggttttcg gtggtcgcgc ctatgagggt gacggtgccc
     1261 tcctcgacgt gggggaggat ggcgtcctgc tgggccttgt tgaagcggtg tatctcgtct
     1321 ataaacagga tcgtgcgtcc ctcgtcgccg gtgatgcgct cggcggcctc cttgacgata
     1381 cggcgcaggt cggccacccc ggcggatacc gcgcttacgg gggcgaagtg ggcgttggtg
     1441 gtattggcga tgacgcgggc gagggtggtc ttgccgctgc cgggcggccc ccagagcacg
     1501 agggagggca cccggtcggc ctctatggcg cggcggagcg tcttaccggg agccagtacg
     1561 tgctcctgcc ccacaatctc gtccagggtg cggggccgca tacgggcagc gaggggggcc
     1621 tcaggctggg gcgagtcggg cgcgggtgcc tccaatttag aacgtttgtc catatcaaca
     1681 ttatagcaaa ccgtgcctgg acgcgcggcg ttttcctcat gtgagggccg ggtgacgaga
     1741 gtggaggaga cttgtcataa attggtcgaa aaactcgtgt aaaagtagtg aagcaaatgg
     1801 aagggtcagc cgttattcta tacagaagaa ctttcctcct ttgggataac cccggcggat
     1861 cgcccaagcc accggggtta gtccttttaa gcgggggttt tccggcttcc ctcttttggc
     1921 ttccccggtc cctccaccaa cctccttgcc cctgcgatgg cgtctttcac ggctgaattc
     1981 tttgtcggtt tcagcacgcc tggcccctct ccgctaacgc ttgtaggtga ccgctcaagg
     2041 tcgctaatct ttcttgcttt ctgcttgccc attgggtata atctgcttct gtggcgcatg
     2101 gcggcagtct cgtactgctg ctgcgggtcg ttgtgtgcaa agtgagggag gagtagggga
     2161 tatgccagtt gtttcgcaga aacggttttc agggattagc ccggcgttgc ttatactagc
     2221 cgggttgttg gcattggtct ttggccttgc cgtgtggctt caacctatta gtgcgggagc
     2281 agccgcgagg ataagtgcgc cgaatgcccc ttcggatacc actccactga cgttgcccgt
     2341 ttcgcagacg tggacgatta cgtcacagat ttcgaccaac gataactggt cgggagtgcc
     2401 cggcttcatg ggctacctgg gagatgaccc cacaactacc gtagcgccgg tcaatcctct
     2461 cagcatcatc accgaaagtg caacaatcga tgtaatagca aaccagaaca acaccgcgat
     2521 caccaacggt ggtgtggccg agtttgagat tacagaccct gtagtagctt tgcaaggttc
     2581 gaacactgcc gatttcccga acatcgttat tcgggtgaat accacaggct ggcagaacat
     2641 caatgtgtcc tacctgatcc gtgacatcga cggcaccgca gacgatgcag cgcaggcggt
     2701 gaacctccag tatcgtgttg gagccagcgg aacgtttacg aacgtgaccg gggggtacat
     2761 tgcggacgcg accactggtg ggaccgcagt gcagacgacg cctgtaagcg ctgtgcttcc
     2821 ggccgccgct gacaaccagc ctatcgtaga tatccgcatc ataaccggca acgccattgg
     2881 ctctgacgag tgggttggta ttgatgacat agccatcttc ggtactgaga ttggtacggc
     2941 gaccaatacc gccgtgccca ccgagacgag cacccctgaa gacactgtca ccggcacgcc
     3001 tcctacggaa accaacacgg ctatccccac cgatactccc atcccgacca acaccagcac
     3061 cgctacacaa acggctacgg ccagcgccac cgccgtgacc actttgcgca tccgcgacat
     3121 acaggactcg tcccacattt cgccctacaa cggtacatcg gtgagcaaca ttcctggtat
     3181 cgttaccggg ataattaccc ctcctggtaa caacaatgat ggcttctact tgcaagaccc
     3241 tacctccgac gcgaatgact cgacgtccga ggccattttc gtccacacgg gcaacgctcc
     3301 taccgtcagc gttggcaact cgttgctcgt tggtggcacc gtttcagagt tcaggcccgg
     3361 cggctcaggc tcggacaacc tgaccctgac tcaaataggt ggtactcttt caataacggt
     3421 gatcagtacg acagccgtgt tgcctactcc tgtggtcatt ggcacgggtg ggcgtattcc
     3481 ccctaccacg atcatcgagg atgacacctc cggcagcgtg gagacaagcg gcgcgttcga
     3541 ccccgcgcag gacggtatcg acttctacga gagcctggaa gcgatgcgcg tgcaggtcaa
     3601 caaccccgtg gcggttggcc ccaggaacgg cttcggcgag atcgccgtgc tcggtgatga
     3661 tggcgccaac gcctccgtgc gcactgctcg gggcggcatc gtaatacaac ccaacgactt
     3721 caaccctgag cggataattc tcgatgacgt catcacgagc actcccgcgg gcgttgacgt
     3781 gggcgaccac ttcaccggcc ccgccgtcgg catcatggac tacggcttcg gcaacttcaa
     3841 gctctacatc acgtccccac tgacggtggt ctccggtaac ctgcaacagg aaaccgcggt
     3901 accggctgtc cagggcgcgc tctccatcgg caactataac gttgaaaacc tcgacccttc
     3961 agattcgact ttcgggctgc acgccagcgc gattatcacc aacctgaagg ctcccgacct
     4021 actctccatc gaggagatac aggacaacag tggctcgacg aacgacggca ccgtcgctgc
     4081 cgacgagacc tgggacgagc tgatcaaggc aattgaagat gcgggcggcc ctacttacga
     4141 gtatcgccag atcaaccctg tgaacaacca ggatggcggc gagccgggcg gtaacatccg
     4201 cgtgggcttg ctgttccgca gcgaccgtgg cctcagcttc gtggacaagc ccggcggcac
     4261 gagcacgaac gcctgcacga tacagccgga cgggaccctc acgatgagcc cctgccgggt
     4321 ggaccccacc aatgctgcct tcaacaacag ccgcaaaccg ctggcaggtg agttcctgta
     4381 taacggcgag acgatcatcg ttatagccaa tcacttcagc tccaagggtg gcgacgaccc
     4441 gctcttcgga cgctggcagc cgcccatacg cagcactgag gcggcgcgtc accagcaggc
     4501 cgcgagtgtc aacggtttcg ttgattcgat cctgaatgcc gatgccggcg ctaacgtggt
     4561 ggtcgccggc gatatcaacg acttccagtt ctcggagacg atgaacatac tcgagaccgg
     4621 tgtgatatcg ggcacgggcg atatcgagct ccagaatctt atcgagaccc tgcccctgaa
     4681 cgaacagtac acctacgtgt tcgacggtaa ctctcaggcg ctggaccacg ctcttgtcag
     4741 cccgaacatg ttcggctacc tggtacagta tgacgtggtg cacatgaacg ccgagttcga
     4801 tccgcaggct agcgaccacg acccgtcggt gggccgcttc aacatgttcg gagccgatac
     4861 ggctactcct actctgacgg ccgttcctag caacacctct acttccacga ccgtgcctag
     4921 ctccaccagc acgagcacga gcaccgctac tcgcacctct acgacggtgc cttccagcac
     4981 gagcactacc gcggctacag ccgtacccag tgccacggct actgcttgca ccatcgagtt
     5041 cgcggacgtg cctcctacag gtgagggttc taccttctac gagttcatcc gctgcctggc
     5101 atgccgaggc atcgtgggtg gctacccctg cggtggacct ggtgagcctt gcaacgagaa
     5161 caacgatcct tactacaggc caggtgccaa cgtcacacg
//
LOCUS       DASRWZ010000445         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1338878, whole genome shotgun sequence.
ACCESSION   DASRWZ010000445 DASRWZ010000000
VERSION     DASRWZ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338878"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VFH60_06925"
     CDS             <1..1279
                     /locus_tag="VFH60_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313551.1"
                     /translation="SVGIIALTSWLARRLLPGHPAWALLPAALLAVDAALLTYGARGS
                     GIEAVPFAFLALAPVAALWAAPVRGWRVVAGVLLGLASLTRPEGLLVAALVLGARAWL
                     DRRDGLPYGKLLAWSVAPFLGMVVPYQLWRITFYGYPFPNTFYAKTGASLAVVGRGAA
                     YAWQFAERHWLLVALSLACLGLLVWAWVSRGRKQGTDPSASDDISPKAPNYGLAATMG
                     LLSVVYSLYVVYVGGDWFPSERFFVPLLAPMSLLAAVGAYALVSRLPRGSRRAVGTAL
                     LGVVVVAYAGYALWQQRPGGELADDTHRHNIYIDRWGSAAIWLRENTQPDTWTAARAA
                     GAIAYYSQRPTIDMFGLNDLHIGHLKVANMGEAVPGHEKSDPAYVLARQPSYVLASWE
                     DYFNPVVEQFKEQYGYETVRSPLGPEVKWLVRK"
     gene            1581..2138
                     /locus_tag="VFH60_06930"
     CDS             1581..2138
                     /locus_tag="VFH60_06930"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6313552.1"
                     /translation="MTNNAEMVILKAEPLEGCDPVVGLALWVLQDSRRRTLGELEELR
                     EEWLDLAPPMGHNTLRSILYHMGSTEVHWLYTVLQQEPPANMKALFPDEDRDEVGNLA
                     RLTGESLAAYLEKMALARQHFIDVYKTMSVEEFRKLRPITHWTGEIHHITPERVLYHL
                     VNHDAEHRGELVMIIQHFKEHSDTK"
     gene            2435..3751
                     /locus_tag="VFH60_06935"
     CDS             2435..3751
                     /locus_tag="VFH60_06935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6313553.1"
                     /translation="MTHDHSQNSRPAPQPFASIVVVSYNTAEHIEGCLRSLLALDYPR
                     SEIIVVDNGSTDGSVELVRRRFPNVELVELGENKGFAGAASVGLFMSKGDILATVNPD
                     VQLDRQWMSAVLDAFDAFPDAGVVGSKILYPDRVTLQHAGGVVHYPLATTEHLGRGEE
                     DRGQYDHSRVVSFVTGAALAMRRDVGHSLNFFDESYYPVFFEDVDLCWRAKREGWRVV
                     YQPGAVAYHNESVTYDRRSSLYYSYFHANRLRFVVKHYTPEQVMLDFLPAEAERLVGD
                     MEREDRKASLSLLDNRAPDGKDRADAEQHNLIARKWDELQRRLDEVLSGWQVYEKSRP
                     RKRRKGLAGRVSNLLSRIYTWPTLRKQIDYNASVARSLMEISRQLADLQARVAVQSIL
                     ASGLVSRQNGAAIDAYAELDALRSRIEELERKSLGATISYGQVRND"
     gene            3729..4193
                     /locus_tag="VFH60_06940"
     CDS             3729..4193
                     /locus_tag="VFH60_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313554.1"
                     /translation="MDKLETTDNGSSADVATVLGDLREQIRQRRAHLAINDPNDPHAL
                     SLAELRRSVEAVNEVWFVSAHLPITWDVRIFGRLLAYAKRVTRLLLRWYINPIVEQQN
                     RFNSATARAVTVMNAYQERMTREWMLLEERVAKLEEEVRGREAGSGGQGDGL"
BASE COUNT          814 a         1311 c         1349 g          790 t
ORIGIN      
        1 cagcgtgggc atcatagccc tgacctcctg gctcgcccgc aggctcttac cagggcatcc
       61 cgcctgggcg ctcctgcccg cggccctctt agccgtagac gcggccctgt tgacctacgg
      121 ggcgcgtggc agcgggatag aggctgtgcc gttcgcgttc ctggcgctgg ctcccgttgc
      181 cgcgttatgg gcagcgcctg ttagagggtg gcgcgtggtc gccggggtgc tgctggggct
      241 ggcctcgctc acacggccgg aggggctact ggtggcggcg ctggtgctgg gtgcgcgtgc
      301 ctggctggat aggcgagacg gcctgccgta tgggaagctg ctggcgtggt cggtggcacc
      361 gttcctcggc atggtcgtgc cttaccagct atggcgcatc accttctacg gctatccctt
      421 ccccaacacc ttctacgcca agacgggcgc gagcctggcc gtggtgggcc gaggggccgc
      481 ttacgcctgg cagttcgcgg agcgacactg gttgttggtg gcgctgtcgc tggcttgcct
      541 gggactgctc gtgtgggcat gggtgtcgcg cggacgaaag caaggtacgg acccatccgc
      601 gtccgacgac atatccccca aagcaccaaa ttacgggtta gcagctacta tggggttgtt
      661 gtcggtggtg tatagccttt acgtggtgta cgtgggcggc gactggttcc cctcggagcg
      721 gttcttcgtg ccgctgctgg cccccatgtc tctgctggct gccgtgggtg cctatgccct
      781 ggtgtcgcgt ctgccgcgag ggagtaggcg ggccgtgggt accgccctgc tgggtgtggt
      841 cgtggtggcg tatgcggggt acgccttgtg gcagcagcga ccgggtgggg agctagccga
      901 tgacacccac cggcacaaca tctacataga ccggtggggt tcggccgcca tctggctgcg
      961 cgagaacacc caacccgaca cctggaccgc cgcccgcgcc gccggggcta tcgcatatta
     1021 cagccagcgc cccacgattg acatgttcgg cctcaacgac ctgcacatag gccatctaaa
     1081 ggtcgccaac atgggcgagg cggtgcccgg acacgagaag tcggaccccg cctacgtgct
     1141 ggcccgccaa ccctcctacg tgctcgcctc ctgggaggac tacttcaacc ccgtggtcga
     1201 acagttcaag gagcagtacg ggtatgagac ggtgcgcagc ccgctggggc cggaggtgaa
     1261 gtggctggtg cggaagtaaa actggcctga tggcagcgta ggcttgggcg ccctgcgtgg
     1321 aatcgtcggg ctgctaggca gcaaagtcac tcacggagac cggggcgtat ctgcaaactc
     1381 aatggaatag ttggtaaact acagtcagga gtctcggacg aaaacatggc gcacctgata
     1441 tgagctatcc gatgaacggc tgaagtggtt gtcacagcct cttccctctt ggcatggaag
     1501 ctccacacca acaaccaaca gaccaggcaa ggaccacagc gcatctgaac agccctcaca
     1561 cgtacaagga gcaacacgta atgacgaaca atgccgagat ggtaatctta aaagccgagc
     1621 ctcttgaagg ttgcgacccg gttgtcggcc ttgccctttg ggtacttcag gactcacgcc
     1681 gcagaacact cggtgagcta gaagagttgc gcgaagaatg gctcgatctc gccccgccca
     1741 tgggccacaa cacccttcgc tccatccttt atcatatggg ttccaccgag gtccactggc
     1801 tatatacggt gttacagcaa gaacctcctg caaatatgaa agcgctcttc cccgacgagg
     1861 accgggacga agtaggcaac ctcgcaaggc taacaggcga aagcctcgcc gcgtacctgg
     1921 agaagatggc ccttgcccgc caacacttta tcgatgtcta caagaccatg tccgttgaag
     1981 agtttcggaa gttgcgcccc atcacccatt ggaccggcga aatccaccac atcacccctg
     2041 agcgtgtact ttatcacctc gtgaaccatg atgctgaaca tcgcggcgaa ctggttatga
     2101 taattcagca cttcaaagag cacagcgaca cgaagtgagc taaccaaaac gcaaaccagg
     2161 gtaagtgcta tctcaaattg tctcggcgct gtggcagcct cacctccgcg tttcctccat
     2221 gttacctgtt tttctctagc tttcattgtc tggcggacgg agtttgcacg tacgccctac
     2281 aagagagtcc ccgcagggga ctttgcctct tgtcagcccg tggctttagc cacaaggcta
     2341 tcgcacgcca cctatgcaga acacctgttg ctctgctcac cttgcatcac gtttcacgtt
     2401 tctgcgttct cgtatataat tcactgccaa aaccatgaca cacgaccaca gccagaacag
     2461 caggcccgcc ccgcagccct ttgcctccat tgtcgttgtt agctacaaca cagcggagca
     2521 tattgagggt tgtttgcgct ctctgcttgc ccttgattac ccgcgctcgg agattatcgt
     2581 ggtggacaac ggctccaccg atggcagcgt agagcttgtg cgccgccgct tccccaatgt
     2641 cgagcttgtg gagcttgggg agaacaaggg cttcgcgggc gcggcaagcg tgggcctgtt
     2701 catgtccaaa ggcgacatac ttgccacggt gaaccccgac gtgcagctag accggcagtg
     2761 gatgtcggcg gtgctggacg ccttcgacgc cttccccgac gccggggtgg tgggcagcaa
     2821 gatcctctac cccgaccgcg tgacgctaca gcacgcgggg ggcgtggtgc attaccccct
     2881 ggcgaccacc gagcacctgg ggcggggaga ggaagaccgg ggccagtacg accactcaag
     2941 ggtggtgtcg ttcgtcaccg gggcggcgct ggcgatgcgg cgcgacgtgg ggcactcgct
     3001 caactttttc gatgagtcgt attacccggt ctttttcgag gacgtggacc tctgctggcg
     3061 ggccaagcgg gagggctggc gggtggtgta ccagcccggg gcggtggcct accacaacga
     3121 atcggtcacc tacgaccgcc ggagcagcct ctattacagc tacttccacg ccaaccggct
     3181 gcgcttcgtg gtgaagcact acacgcccga acaggtgatg ctcgatttcc tgcccgccga
     3241 ggccgagcgt cttgtgggcg atatggagcg ggaggaccgc aaagcctccc tcagcctgct
     3301 ggacaaccgc gcgcccgacg gcaaggacag ggccgacgcc gagcagcaca acctcatcgc
     3361 gcgcaagtgg gacgaactgc aaaggcgact ggacgaggta ctgagcggct ggcaggtgta
     3421 cgagaagtcg cgcccgcgca agcgtcgcaa gggcctagcg ggtagggtga gcaacctgct
     3481 gagccgcatc tacacgtggc ccacgttgcg caagcagata gactacaacg cctcggtggc
     3541 ccgctcgctg atggagatct cgcgccaact ggccgatttg caggcccgcg tggcagtgca
     3601 gtcgatactg gcgtcggggt tggtatcgcg gcagaacggc gcggcaatcg atgcgtacgc
     3661 cgagctggac gcactccgct cgcgaattga agaattggaa cgtaagagcc tgggtgccac
     3721 tatcagctat ggacaagtta gaaacgactg acaacggctc gtccgccgat gtggccacgg
     3781 tgttgggcga cctgcgcgag cagatacgcc agcgccgcgc ccacctcgct atcaacgacc
     3841 ccaacgaccc ccacgcgctg agcctggccg aactgcgccg cagcgtggag gcggtaaacg
     3901 aggtctggtt cgtgtcggcc cacctcccta tcacgtggga cgtgcggata ttcggcaggc
     3961 tgctggccta cgccaagcgc gtgacgcgcc tgctgctgcg ctggtacatc aaccccattg
     4021 tggagcagca aaaccgcttc aactcggcca ccgcgcgcgc agttaccgtg atgaacgcct
     4081 accaggagcg catgacccgc gagtggatgc tgctggagga gcgggtggcg aagctggagg
     4141 aggaggtcag gggccgggaa gcgggcagcg gaggccaggg agacggcctt tagtgggcca
     4201 cgaaccacac gccgcaaggt ttgctcccca ggaggatacg gcaggccctt caccggcagc
     4261 ttcg
//
LOCUS       DASRWZ010000446         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1202484, whole genome shotgun sequence.
ACCESSION   DASRWZ010000446 DASRWZ010000000
VERSION     DASRWZ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202484"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="VFH60_06945"
     CDS             complement(<1..1413)
                     /locus_tag="VFH60_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313555.1"
                     /translation="MLPSLFILIITALYCAALGLVPGRSDLARWVAVAGMGAALVVLV
                     ARGTLDTAGASVAWPGWLGWLGEPVYRDDALGAGLGAWCLLLGLLCLLKLDSCEAAAW
                     QTANAVSIVAVLFSLAHTDHLLAFAGQLFLLAALLLAGQTLQGGLPTDFSRAAVGLGL
                     GALLVLGAALLVGRATGGAYRLSDLPLSALTAWPLTLLGGFVVLWLGMPPFTGWSGRG
                     SGGVVSATLAQSLAVGVPVVVLVLRLQGLVSGQAFVGSVPGPWASLMGVLAWFGAAGA
                     LVAACGLLVWAGSARWTSMLTAYSLGMALWALALDNPAGRLAALVVLLSYGAGRVTLL
                     LAGDGASTWERMARAVAIASLAFAPLTASFVGTWLLASTLSAEGNASTALALVGAAIV
                     AACGIVLHYAATDRPTTEAASPPLLAWLASGAALGLVAGGTLPGLWLPYVARVAETGG
                     GVPVVSLPWQGVGAGGDFSPV"
     gene            complement(1744..2904)
                     /locus_tag="VFH60_06950"
     CDS             complement(1744..2904)
                     /locus_tag="VFH60_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313556.1"
                     /translation="MHTIQDSNTGTGRAALDIEVGPYDPALPGAMLLRLGLEPGGGSS
                     PLGWSTRIVGAEVELGYNRVGLEERAALGGLDWAEGLQLVMSLCGCCSQANSLAYVQA
                     VEDLSGALVPPRAAYLRLVLAEAERVTSHLLNAADTLAMLGYAEREAAIRDMRERILH
                     ALGEFTGGRLQQGLIVYGGVSRNLDEAACRAFTIAARNVERTLRGHIRSVIKDREIAR
                     RLAGLGRVTAEEAAFAGLRGPIARAAGVTYDIRAHFPTGAYEDEAVTVVVQRAGDAYA
                     RLVVRLLECLESFRVMEQALDDLPPGPVKSRGSLDFGLGEGGSGIGRAEGPRGEVFCW
                     ARGDGEGLQGLHLSSGSQATLGVLPGLLAGTELDDLPLLLLSLDLCLPCAER"
     gene            complement(3164..3661)
                     /locus_tag="VFH60_06955"
     CDS             complement(3164..3661)
                     /locus_tag="VFH60_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313557.1"
                     /translation="MANSYGSGWAGGAGGAEEQVERQLASFAFVREEREREVRLHFRD
                     VSAGELAMSFRRSGASLISIMGERARVVAAPAAEEASATQADEEEHAGGFRRRRRSHS
                     ESASTPPGELTMRYFYSLGELVYTISITVPSGVVQSVAGIYPNAALPERELQEHLGVV
                     FLSNE"
     gene            3908..>4205
                     /locus_tag="VFH60_06960"
     CDS             3908..>4205
                     /locus_tag="VFH60_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313558.1"
                     /translation="MRVALNGFFWDQPLTGSGQYLRHLWAALSEIAQHTPSPGRSTKG
                     PSFSLLLPPSAKQDGPEPVPTLPGFSMHKARPLPLGPVSGRSKNLDQLRWEDWGV"
BASE COUNT          786 a         1579 c         1207 g          633 t
ORIGIN      
        1 cacgggcgag aagtcacctc ccgcgcctac accctgccag ggcaggctca caacaggtac
       61 gccacccccc gtttcggcta cgcgcgccac gtatggcagc cacaatccgg gcagcgtgcc
      121 ccccgccaca agccccagcg cggctccaga tgctaaccac gccaggagcg gcggagaggc
      181 agcttcggtc gttgggcggt ccgtggcggc atagtgcagc acgataccgc atgccgccac
      241 gatagccgcg cccaccagcg ccagcgccgt cgaagcgttc ccttcggcgg acagcgttga
      301 ggccagcagc caggtgccca cgaacgaagc ggtgaggggc gcgaacgcca gcgaggctat
      361 agccaccgcg cgggccatgc gctcccaggt ggacgcgccg tcgcccgcca gcaggagggt
      421 cactcgcccc gcgccgtaac tcaacaggac caccagggcc gctaggcgtc cggcggggtt
      481 gtcgagggcc agcgcccata gggccatacc caggctgtag gcggtgagca tggaggtcca
      541 gcgcgcgctg cccgcccaca ccagcaggcc gcaagccgcg accagagcgc ccgccgcgcc
      601 gaaccaggca agcacgccca tgagcgaggc ccacggcccc ggtacgctgc ccacgaacgc
      661 ctggccgctg accagccctt gcagccgcaa caccagcacc actaccggca cgcccaccgc
      721 cagcgactgc gcgagcgtgg cacttaccac accgcccgac ccacgcccgc tccagccggt
      781 aaagggcggc atgccgagcc acagcacgac gaacccaccc aggagcgtga gcggccacgc
      841 ggtgagcgcc gacagcggca ggtcgctcag cctgtatgcc ccgcccgtcg cccggcccac
      901 gagcagcgcc gcgccaagca ccaggagcgc gcccagcccc agccccaccg ccgcgcggct
      961 gaagtcggtg gggaggccgc cctgcaaggt ctgcccggcg agcagcagcg ccgcgagcaa
     1021 gaatagctgc ccggcaaacg ccagcaggtg gtctgtgtgc gcgagactga agagcacggc
     1081 cacgatggat accgcgttcg cagtctgcca ggccgcagcc tcgcagctat cgagcttgag
     1141 caggcacagc aaccccaaca ggaggcacca ggccccaagc cccgccccaa gcgcgtcgtc
     1201 ccggtagaca ggctctccca gccagcccaa ccatcccggc cacgcgaccg acgcccccgc
     1261 cgtatcgagc gttccgcgcg ccaccagcac gaccagggcc gcccccatgc ccgctaccgc
     1321 cacccagcgc gccaaatcgg accgtccggg caccagccct agcgcggcgc agtacaaggc
     1381 tgtgattatg aggatgaata gggaaggcag catcacttgt cgattatccg taacccatcg
     1441 caacagccac tttcgggcac tctacgcccc tgcctattat tccggtacaa actccatcta
     1501 caagaccaaa atcggccctg tcatcctgag cgaccctttg tcatcctgag cgtagcgaag
     1561 gatcagccac ccgtgtttga ctcttcctat tgatgtaccc ctgtgcctct agccgccatc
     1621 atcctgagcg ttgtagactc tcgcctaccc acctgatcct tcgctacgct caggatgaca
     1681 aaagaggctc aggacgataa gggcaagcaa tctcccttta gtcccaacac gtgtaagccg
     1741 gggtcacctc tccgcgcagg gtaggcacag gtcgagcgag aggagcagga gaggcaggtc
     1801 gtccagctcg gtgcccgcga gcaggccggg aagcacaccc aacgtcgctt gcgagcctga
     1861 gctgagatgg aggccctgca agccctcgcc atcgcccctc gcccagcaga atacctcgcc
     1921 tcgcggcccc tcggcccggc ctatgccgct cccgccctcg cccagcccga agtccaggct
     1981 gcccctcgat ttcaccggcc ccggcggcag gtcgtccagc gcctgctcca tcacccggaa
     2041 gctttccagg cactccaaca gccgcaccac cagccgcgcg tacgcgtccc ccgcccgctg
     2101 caccaccacc gttacggctt cgtcctcgta cgcgccggtg gggaagtgcg cgcggatgtc
     2161 gtaagtgacc ccggcggctc tagcgatggg gccgcgcaac cccgcgaaag ccgcctcctc
     2221 cgccgtgacc ctgcccagcc cggccagcct gcgggcgatc tcccggtcct tgatgaccga
     2281 ccggatgtgc ccgcgcaggg tgcgctcgac gttgcgggcg gcgattgtga aggcccggca
     2341 ggccgcctcg tccaggttgc ggctcacccc cccgtacacg atcaagccct gctgcaagcg
     2401 cccgcccgtg aactctccca gtgcgtgcag tatgcgctcg cgcatgtcgc gtatggccgc
     2461 ctcgcgttcc gcgtacccca gcatcgccag cgtatcggct gcgttcagca agtgcgacgt
     2521 gacccgctcg gcctcggcca gcaccaggcg caggtaggcg gccctcggag gcacgagcgc
     2581 ccccgacagg tcctccaccg cctgtacgta ggcgagggag ttggcctgcg agcagcaacc
     2641 gcacagcgac attacaagct gcaacccctc ggcccagtcg aggcccccca gggccgcgcg
     2701 ctcttccagc cccacccggt tgtagccaag ctcgacctcg gcacccacaa tgcgcgtgct
     2761 ccaacctagc gggctgctcc ccccgcccgg ctctagcccc aaccgcaaca gcatagcccc
     2821 aggcagcgca gggtcgtacg gccccacttc aatatcgagc gcagccctgc cggtgcctgt
     2881 gttgctgtct tgtattgtgt gcataggtta gttatgagtt atgagtaatg aggactgagt
     2941 tgtagataga cctgtgatgt cgcctggatg cgctatggtg tggccttcac cctgccaatg
     3001 aattggcggg ctgaccagag acaaagtctc cttcggagac tacatgacgt tcctaagtcc
     3061 ccgcagggga ctttgcccct tgtaagcccg tggctttagc cacagggcta tcgcagggcg
     3121 tcctccaccc cttgtgtctc gccctttcgc aacccattac tcattactca ttactgagaa
     3181 acaccactcc caggtgctct tgcaactcgc gctccgggag agcggcgttc gggtatatgc
     3241 ctgcgaccga ttggactacg cctgacggca cggtgatgct tatagtgtag acaagttcgc
     3301 ccagggaata gaagtagcgc atcgttagct caccgggggg tgtagaagcc gactcggagt
     3361 gagacctgcg ccttctgcgg aagcccccgg cgtgctcctc ctcgtcggcc tgcgtcgcgg
     3421 aagcctcctc agcagcgggc gcggctacca ccctggcgcg ctcgcccatg atgctgatga
     3481 ggctcgctcc cgaccggcgg aacgacatag caagctcgcc cgccgacacg tcgcgaaaat
     3541 gcaggcgcac ctcccgctcg cgctcctcgc gcacgaaagc gaagctggca agctgccgct
     3601 ctacctgctc ctccgcgcct cccgcgcctc ccgcccaacc ggacccgtag ctgttcgcca
     3661 caacaaatcc cccggttata agtgtcgagc gccaggtgcc gagtaaggca tggcataccg
     3721 gcaccgtcat tatgcacacg gactatacca gcccagcgga tgagcgtcaa gatggccggc
     3781 acccaggcat ctatagtcgc ttgctatgcg ctaccgaatt acggaaaacg aagtacccaa
     3841 ccactctcac catcaaattc gcaatccgca atccgcaatc ccaaattccc tgtgctataa
     3901 tgtgctcatg cgcgtggcgt tgaacggctt cttttgggac caacccctca ccggctcagg
     3961 acaatacctc agacacctct gggccgccct atccgagatc gctcagcata cgccgtcccc
     4021 cggtcgatct accaaaggcc cctccttttc cctgctgctc ccgccctccg cgaaacagga
     4081 cgggcctgaa cccgtaccca cgctcccagg cttcagcatg cacaaggccc gtcccctgcc
     4141 gctcggcccg gtctcgggtc gaagcaagaa cctcgaccag ctgcggtggg aggactgggg
     4201 tgtca
//
LOCUS       DASRWZ010000447         1991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1065369, whole genome shotgun sequence.
ACCESSION   DASRWZ010000447 DASRWZ010000000
VERSION     DASRWZ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065369"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1063)
                     /gene="ispE"
                     /locus_tag="VFH60_06965"
     CDS             complement(191..1063)
                     /gene="ispE"
                     /locus_tag="VFH60_06965"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET6313559.1"
                     /translation="MRAGAMLELAAHAKINLGLEVVGKRGDGYHDLVSVMQLVDLHDT
                     LVFAPAQDIRLLSDDPAMQAEGEDNLVLRAAHLLRQTYGVDDGVSITLHKRIPTAAGL
                     GGGSSDAATTLLGLSRLWGLDLPREELARLGGTLGSDVPFFFAGPTALVEGRGERVTP
                     VAPSPPFQAVLVSQPYSLPAKTRQLYASLTPADMSDGSATRELLEILRQRGDITALSL
                     YNSFERAALQVFPGLDNVRQRMLDAGAPNVHLSGSGPTLYALYPSSAASEAQLLHDAM
                     TQAGFRAYLVASET"
     gene            complement(1095..>1991)
                     /gene="rsmA"
                     /locus_tag="VFH60_06970"
     CDS             complement(1095..>1991)
                     /gene="rsmA"
                     /locus_tag="VFH60_06970"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET6313560.1"
                     /translation="PGEAGADDDYLELGTRHSALGTRHSALPHHRPRKSLGQHFLRDR
                     SVPPRIATAAAIEPGDWVVEVGPGLGVLTEELARRLDPERGKLVAVELDDDLLPVLRE
                     RFAPMPHVSFVHADVLNVPPQELSQGHPYKLVANLPYYITSAILRHFLEAEHKPRSLT
                     VMVQKEVADRMAARPPEMSLLAVSVQFYGRPKIMFRVPPGAFNPPPKVDSAVVRIDVY
                     GEGENPVEVSSEAGFFRLAQAGFGQRRKQLANTVASGLGLPKGEVVALLQRAGIEPSR
                     RAETLTLEEWARLERTLSGIRI"
BASE COUNT          353 a          655 c          641 g          342 t
ORIGIN      
        1 gagcgtagcg aaggatctaa gatgctccta ttgttctgtg gctgaactcc taccgccttt
       61 ggtcgtggaa gttcggcacg actgcggccc aacttttgga gatccttcac tacgttcagg
      121 atgacagaaa agagcatagt cctgctatgc caggcgggcc atgttcacgt accggccgtc
      181 gcaaggctcg tcacgtctcg ctcgcgacga ggtacgccct aaatccggcc tgtgtcatgg
      241 cgtcgtgcag gagttgcgct tcgctagcgg cggaacttgg gtagagcgcg tacaacgtgg
      301 gaccgctgcc ggagaggtgg acgttagggg caccggcgtc cagcatccgt tgtcgcacat
      361 tgtcgaggcc ggggaagacc tgcaaggcgg cgcgctcgaa agagttgtac agggacaggg
      421 cggttatgtc gccgcgctgc cgtaaaatct ccaatagctc gcgtgtggcg ctgccgtcgc
      481 tcatgtcggc gggcgtgagg ctggcgtaaa gctgccgggt cttggccggg aggctgtagg
      541 gttgggagac cagcaccgcc tggaaggggg gcgacggcgc taccggggtg acacgctcgc
      601 ccctgccctc gaccagcgcg gtaggtccgg caaagaagaa cggcacgtcc gagccgagcg
      661 tgccgcccaa tcgcgccaac tcctcgcggg gcaagtcgag gccccacagc cgcgacaggc
      721 ccagcagagt ggtggccgca tcgctgctcc ccccacccag cccggcggca gtggggatgc
      781 gcttgtgcag cgtgatgcta acgccgtcat cgacgccata cgtctggcgc aataggtggg
      841 cggcgcgcag cacaaggttg tcctcccctt cagcctgcat ggcggggtca tcgctgagca
      901 accttatgtc ttgcgcgggg gcgaagacga gcgtgtcgtg caggtccaca agctgcatca
      961 ccgacacgag gtcgtggtag ccatcgcccc gcttgcccac gacctcaagc cccaggttta
     1021 tctttgcgtg ggccgctagc tccagcatcg caccagcccg cataaatgtc tgagcaaatc
     1081 ctaaatccta aatcctaaat cctaattcca ctcagcgtcc gttccagccg cgcccactcc
     1141 tccagcgtga gggtctcggc ccggcgggac ggctcgatac cggccctctg caaaagtgca
     1201 acgacctcgc ccttcggcag gcccagcccg gaggccaccg tattcgcgag ttgcttcctg
     1261 cgctggccga accccgcctg cgccagccgg aagaaccccg cctcgctgct cacctccacc
     1321 gggttctccc cctcgccgta cacgtcgatg cgcaccacgg cggaatcaac cttaggaggg
     1381 gggttgaacg cgccgggcgg cacgcgaaac atgattttgg gcctgccgta gaactgcacc
     1441 gacaccgcca gcaggctcat ctcagggggc cgggccgcca tgcggtctgc cacctctttc
     1501 tgcaccatga cggtgagaga gcggggcttg tgctcggctt cgaggaagtg gcgtaggatg
     1561 gccgaggtga tgtaataggg caggttcgcc accagcttgt acgggtggcc ctgcgaaagc
     1621 tcctggggag gtacgttcag cacgtcggcg tgcacgaagc tgacgtgggg cataggagcg
     1681 aaccgctcgc gcagcaccgg cagcaggtcg tcgtccagct ccaccgcgac cagcttgcct
     1741 cgctccgggt ccaacctgcg ggccagttcc tcggtgagca ccccaagccc cggccccacc
     1801 tccacgaccc agtcgcccgg ctcgatagca gccgcagttg cgatcctcgg cggcaccgag
     1861 cggtccctca ggaaatgctg ccccagcgac ttgcgaggac gatggtgggg aagtgccgag
     1921 tgccgagttc cgagtgccga gtgccgagtg ccgagttcca agtaatcgtc gtcagcccct
     1981 gcctcacccg g
//
LOCUS       DASRWZ010000448         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_106145, whole genome shotgun sequence.
ACCESSION   DASRWZ010000448 DASRWZ010000000
VERSION     DASRWZ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106145"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..2380
                     /locus_tag="VFH60_06975"
     CDS             44..2380
                     /locus_tag="VFH60_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HET6313561.1"
                     /translation="MHTTNSTQQTAAEHKRDGNGHRGGIDRLSLIIDATSVLVAYIDR
                     HYLLTFANAAFARWYGRTPDEITGKPLVEVLGEGEYEAVRGMLEAGMGGRTLDFEVSI
                     PWADKETRYLQVSYVPEVDEQGQVLGVIASMSDVTESKLAEADLRHREQELRDFIENG
                     AISLHWVGPDGTILWANRAELQLMGYTEEEYIGRNISDFHADPVVINDILRRLSDKET
                     LHSYEARLRAKDGSLKHVLISSNVLWDEEGRFVHTRCFTRDITERKQAEEALRESEER
                     NRAVLEQVSEAIFLLDPETRRFVTANPAFQRLLGYTETELAGMTIYDVLPYERERIDR
                     TIQRALDSEEYHVSDRLYKRKDGSLVNVEVGITSVSYGGKKVLCQVIRDITERKRIEA
                     ELRANEERFQSLSTCSPVGIFMTDTQGHATYSSLRCQAICGFTFEQSLGLGWTEYIHP
                     DDRSRVLADWSSAAQAGREYAGEFRFRAGGGTDRWVHMRTAPMLSVNGELIGHTGTVE
                     DISERRLSEEERNQLLVSEQAARADAEEALRMRDELLAIVSHDLKNPLAVIKGNIQLA
                     RRRIEQTNGSDPSRVMSILERIDDAQAKMNMLINELMDFGRLQAGLPLTLQRRQVDLV
                     SLVHEVVMEQRHTATRHNIRMQVGMQELVGWWDAERLERVISNLLSNAIKYSPSGGDI
                     TVEVAHESVAGEESAEGADTWAVLTVRDQGLGISSEDMPHIFEWFRRASDVSGRISGA
                     GIGLASSLRAVQQHGGTISVESEPGVGSSFTVRLPVGT"
BASE COUNT          569 a          818 c          886 g          478 t
ORIGIN      
        1 aaataatgaa cgcttcgcta cccttctcag acaaggcgct attatgcata ctactaactc
       61 cacacaacag accgccgcag agcacaagcg agatggtaac ggtcacagag gcggcataga
      121 tcgcctttcc ctcattatcg atgctacctc cgtgctcgtc gcatacatag acaggcatta
      181 cctgcttact ttcgcgaacg ccgctttcgc acggtggtac gggcgcaccc ccgatgagat
      241 taccggcaag ccgctggttg aagtgcttgg cgagggcgag tatgaggctg ttcgcggcat
      301 gctggaagca ggcatggggg gccgcacgct agactttgag gtctcgatac cctgggcgga
      361 taaggagacc aggtacttgc aggtgtccta cgtgcccgag gttgacgagc agggccaggt
      421 gctcggcgtg atcgccagca tgagcgacgt taccgagagc aagctggccg aggccgacct
      481 gcgccaccgc gagcaggagc ttcgcgactt catcgagaac ggagccatca gcctccactg
      541 ggttggcccc gacggcacaa tcctgtgggc caatcgagcc gagcttcaat tgatgggcta
      601 caccgaggaa gagtacatcg gccgcaacat ttcggacttc cacgccgacc cggtcgtaat
      661 caacgacatc ctccgcaggc tctccgacaa ggaaacgctg catagctacg aagcacgcct
      721 cagggccaaa gacgggtcgc tcaagcacgt gctcatcagc tccaacgtgc tgtgggacga
      781 ggaagggcgt ttcgtccata ctcgctgttt tacccgcgac atcaccgagc gcaagcaggc
      841 ggaggaggcg ctgcgggaga gcgaggagcg caaccgcgcc gtgctcgaac aggtctcaga
      901 ggcgatcttc ctgttagatc cggagacccg ccgattcgtg accgccaatc cggcgttcca
      961 gaggctgtta ggctacacgg agacggagct tgcgggcatg accatctacg acgtgctgcc
     1021 gtacgagcgc gagcgcatag accgcaccat ccagcgcgcc ctcgactcgg aggagtatca
     1081 cgtgagcgac cgcctctaca agcgcaagga cggctcgctg gtcaacgtgg aggtgggcat
     1141 cacctcggtg tcttacggcg gcaagaaggt gctttgccag gttatacgcg acatcaccga
     1201 gcgcaagcgc atcgaggccg agctacgcgc caacgaggaa cgcttccagt cgttgagcac
     1261 ctgctcgccc gtgggcatct ttatgaccga tacccagggc cacgcgacct actctagcct
     1321 gcggtgccag gctatctgcg gctttacgtt cgagcaaagc ctgggcctcg gctggaccga
     1381 gtacatacac ccagacgaca ggtcgcgcgt gctggcggac tggtcttcgg ccgcgcaggc
     1441 gggccgtgag tatgccgggg agtttcggtt tcgcgcgggg ggaggcacgg accgttgggt
     1501 gcatatgcgc acggccccca tgctgtcggt gaacggcgag ctaataggcc acacgggcac
     1561 cgtggaggac atcagcgagc gcaggctgag cgaggaagag cgcaaccagc tcctggtcag
     1621 cgagcaggcc gcccgcgccg acgccgaaga agccctccgc atgagggacg aactgctcgc
     1681 catcgtctcg cacgacctca agaatcccct ggcggtgatt aaaggcaaca tccagcttgc
     1741 caggcggcgc atcgagcaaa cgaacgggtc ggacccctcg cgggtcatga gcatcctgga
     1801 gcggattgac gacgcccagg cgaagatgaa catgctcatc aacgagctaa tggacttcgg
     1861 gcgcttgcag gcggggctgc cactcaccct ccaacggcga caggtagacc tggtgtccct
     1921 cgtgcacgag gtagtaatgg aacagcggca cacggccacc cggcacaaca tccgtatgca
     1981 ggtgggcatg caggagctgg ttggctggtg ggacgccgag cggctcgaac gggtaatctc
     2041 caacctgctg tctaacgcta tcaagtacag ccccagcggc ggcgatatca cggtggaggt
     2101 ggcgcacgag agcgttgcgg gcgaggagag cgccgaaggg gccgacacgt gggccgtcct
     2161 caccgtgaga gaccagggcc tcggcatctc ctccgaggac atgccccaca tattcgagtg
     2221 gttccggcgg gcgagcgacg tgtcggggcg cattagcggc gcgggcatcg ggctggcaag
     2281 ctcgctccga gccgtgcagc aacacggggg cacgatcagc gtggagagcg agccgggggt
     2341 ggggtcgagc tttacagtgc ggctaccggt cggcacgtag ggggcggggc cgcgagggag
     2401 gggagttggg ccaggctgcg ctgtgggggc aacgtgcgag aatcctgcgg gtgtgccctg
     2461 catgacgccc tgccactaaa ttggcgggct gaccagagac gtagtctcct tcggagacta
     2521 ccatgatgtt gcttagtccc cgtaggggac tttgcttctt gtaagccggc cactttagtg
     2581 gcagggcgtc atgattaagt cctgggcaca agaagtgcgc gcttagattt tattcctgca
     2641 aagctgctat aattctaacc aacatacaat gagacagcac gaacattcct cctttacggc
     2701 ttacgtgtta cgttttacgt gttacgcgtt acgccttagc gacctttcca c
//
LOCUS       DASRWZ010000449         4511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_37092,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000449 DASRWZ010000000
VERSION     DASRWZ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37092"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1320
                     /locus_tag="VFH60_06980"
     CDS             235..1320
                     /locus_tag="VFH60_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET6313562.1"
                     /translation="MAPEPYTPEDQGTASAPASASPSQPLYVLIDHQLDAQYIEQIEA
                     ANANVRVLTTFPNTGVADPMRDGQNARRLEGEALDEVLGQAEVLFAFGFPVEWLHRAP
                     RLKWVQLASAGSDQMQRAGIFDLRPDLMLTTSSGVHEVPISEHIVGMLLYFARGFQLA
                     VRNQPAHKWERYQANEAAGRTVCFIGYGPIARRAAFLCKTLGMQVLVVRASITEEQPG
                     NEHVDRYYPRALLGRALAQSDYIVVAAPRTPATEGMISSEQIASAKDGAVLINISRGA
                     LVDEDAMIEALQNGKLRGAGLDVFREEPLPPGSPLWDMPNVLITPHVSGSSPHYNERA
                     TAIFADNLGRYTRNEPWRNMVIKERGY"
     gene            1414..4431
                     /locus_tag="VFH60_06985"
     CDS             1414..4431
                     /locus_tag="VFH60_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HET6313563.1"
                     /translation="MPRTYIAFDLEATGMQPERDEVIEIGAVKFRDGQVIGRWESFVR
                     PTQQIPYKVQQLTGIKPADVARAPRISEVAADFIRFVGDSPVIGQSVELDLAMLERHG
                     VRLRNVAWDTFELATLLVPEAAVYNLRNVAEKLGVSQEVRAHRAVADAELTMRVFIAL
                     RERIAELPLEVLSDIGKATNRSEWPLRLLFQEVEHEKAQVAFSSSIGSSIRAQLQAKG
                     LTDAELDVGLLRQPDEDMQYAPVGAGFEGKFPLDHTEIENIFKPGGAMSKTFAGYEYR
                     PQQLEMAKAVASSLNKGRHLIVEAGTGTGKSLGYLLPAVLYALRTGEQVVVSTNTINL
                     QDQLFTQDLPLLHRVLEGPPPDKKSGKQQIQNPKPKIQNGDAVIQEPFHAALLKGKGN
                     YICLRRWYQFRRNVPSSVEQLRVMVKVLIWLPQTETGDRNELLLLNEENDAWGHLRVT
                     EEGCPPFECRVRQKGLCFFDRARRRAYGANVIVVNHALLLADLAMGGGVLPEYENLII
                     DEAHNLEDEATDALGFTVDRRGLVRLLEDLSSGSAARHREDDFLTALRNALAADVSKG
                     LKTKGGSRVEAGATRNLLTGVDDVIAKLRPSIERARQSAVELFTQLNSVMEVYQDEQN
                     VYDLRQRITNEVRRHATWGQVEMIWDNLGLQLKDIEDGLSRIGTVLGDVDWDNIPRSG
                     TGDVPAYADMLIELRGLITNVHKLRADANAGITNPDEGAVYWLEARVKGGDVALRCAP
                     LHVGELLDRHLFSKKRAVVLTSATLSTDNDFSYILERLGLHSCEQLQLSSPFDYQKAA
                     LVYLPTDMPEPSTPGYQRSLEQALVELCKASQGRTLALFTSHSAVRATYRAIQRPLEE
                     AGIMVLGHNIDGSRKQLLERFKNNPRTVLLGTSSFWEGIDVVGDALSVLVIAKLPFAV
                     PTDPVFAARAEGFEDAFLQYSVPQAILKFTQGFGRLIRSRNDRGVVAVLDRRVISKRY
                     GQAFLNSLPEATVRHGLTANLPGEVADWLGHKW"
BASE COUNT          892 a         1347 c         1535 g          737 t
ORIGIN      
        1 actttgcctc ttgtaagccc gtggcttcag ccacagggcg tgcgtggcag ggcgaatgcc
       61 acagggcatc tccaccccag cctgcggaga atgattaagc tttggaaggt gaagtgcaac
      121 ggattgcgct tggcacccgc atggtgtaga atgagcgtcg gcacggctct tcggagcccc
      181 tttcgctttc cagcgccgcc ggcgccgagc acgcggctag aaagagtagc acacatggcc
      241 cccgagccat acacacccga agaccagggc accgcatccg cccccgcatc cgcctcaccg
      301 tcccaaccct tgtacgtact catagaccac cagctcgacg cgcaatacat cgagcagatt
      361 gaggctgcga acgctaacgt gcgggtactg accacgttcc cgaacacggg tgtggccgac
      421 cctatgcgag acggccagaa cgcgcggcgg ctggaggggg aagcgcttga cgaggtgctg
      481 gggcaggcgg aggtgctgtt cgcgttcggc ttccccgtag agtggctgca cagggcgccg
      541 cgcctcaagt gggtgcaact ggcaagcgcc gggtcggacc agatgcagcg ggcgggtatt
      601 ttcgacctgc ggcccgattt gatgctcacc acaagcagcg gcgtgcacga ggtgcctatc
      661 tccgagcaca tagtgggcat gctgctatac ttcgctcgcg gcttccagtt ggctgtgcgc
      721 aaccaaccgg cgcacaagtg ggagcggtac caggcaaacg aagcggcggg caggacggtg
      781 tgcttcatcg gctacggccc gatagcgcgg cgggcggcgt tcctgtgcaa gacgctcggc
      841 atgcaggtgc tcgtcgtgcg cgcctcgatt acggaggagc agcccggtaa cgagcacgta
      901 gaccgctact accctcgcgc cctgctgggc cgggcgctcg cgcagagcga ctatatagta
      961 gtagccgccc cgcgcacccc cgccaccgag ggtatgataa gtagtgagca gatcgcctcg
     1021 gccaaagatg gggcggtgct catcaacatc agccgtggcg cgcttgtgga cgaagacgcg
     1081 atgatagagg ctctgcaaaa tgggaagctt cgcggcgcgg ggctggacgt gttcagggag
     1141 gagccactgc cccccggcag ccccctgtgg gacatgccca acgtgctgat aaccccacac
     1201 gtctctggct cgtcccccca ctacaacgag cgcgccaccg ccatcttcgc cgacaacctg
     1261 ggacgctaca ccaggaacga accgtggagg aacatggtta tcaaggagag aggatactga
     1321 ttgattttgg attttagatt ttggattttg gattcgcccc ctcaatctaa gatctaaaat
     1381 ctaagatcta aagtctaaaa tctaaaatct aaaatgccaa ggacttacat tgcgtttgac
     1441 ctcgaagcta ccgggatgca gccggagcgg gacgaggtaa tcgagattgg ggctgtgaag
     1501 ttccgggacg ggcaggttat tggccgttgg gagagcttcg tgcgccctac gcagcagatc
     1561 ccgtataagg tgcagcagct cacgggtatc aagcccgcgg acgtggcccg cgcccctcgc
     1621 atatccgagg tggccgccga tttcataagg ttcgtggggg actcgccggt catcgggcag
     1681 tcggtggagt tggacctggc gatgctggag cggcacgggg tgaggctgcg caacgtagcg
     1741 tgggacacat tcgagcttgc cacgctgctc gtgccggagg cggcggtata caacctgcgc
     1801 aacgtggccg agaagctggg ggtgagccag gaggtacgcg cccacagggc cgtggccgac
     1861 gccgagctga ccatgcgggt gttcatagcg ttgcgggagc gaatcgcgga gctaccgctg
     1921 gaggtgctga gcgacatcgg caaggccacc aaccgcagcg agtggccgtt gcgcctgctg
     1981 ttccaggagg tggagcacga gaaagcccag gtggccttct caagcagcat cggctcgtcc
     2041 atacgcgctc agttgcaggc caaggggctg accgacgcgg agctggacgt ggggctgttg
     2101 cggcagccgg acgaggatat gcagtacgca cccgtggggg cgggcttcga gggcaaattc
     2161 ccgctcgacc acacggagat agagaacatc ttcaagccgg gcggggcgat gtcgaagacg
     2221 tttgccgggt acgagtaccg cccgcaacag ctagaaatgg cgaaagcggt ggcaagctcc
     2281 ctcaacaagg ggcgacacct gatagtggaa gcgggcacgg gcacgggcaa gagcctgggc
     2341 tacctgctac ccgccgtgct ctacgccctg cgcacgggag agcaggtggt ggtctccacc
     2401 aacaccatca acctgcaaga ccagctcttc acccaggacc tccccctcct ccaccgcgtt
     2461 ctagaaggcc ccccacccga caagaaatca ggcaaacaac aaatccaaaa tccaaaaccc
     2521 aaaatccaaa atggcgacgc agtcatccag gagcctttcc acgccgcgct gctgaagggg
     2581 aagggcaact acatctgcct gcggcgatgg taccagttcc ggcgcaacgt gccgtcgtcg
     2641 gtggagcagt tgcgggtgat ggtgaaagtg ctcatctggc tgccgcagac cgagacgggc
     2701 gaccgcaacg aactgctgct gctgaacgag gaaaacgacg cctgggggca tttgagggtg
     2761 accgaggagg ggtgcccgcc gttcgagtgc agggtgcgcc agaaggggct gtgcttcttc
     2821 gacagggcgc ggcggcgggc gtacggggcc aacgtcatcg tggtgaacca cgccctgctg
     2881 ctggccgacc ttgcgatggg cggcggggtg ctgccggagt acgagaacct catcatcgac
     2941 gaggcgcaca acctggagga cgaggcgacc gacgcgctcg gcttcacggt ggaccggcgg
     3001 ggattggtga ggctgctgga ggaccttagc agcggctcgg ctgcccggca cagggaggac
     3061 gacttcctca cggccctacg caacgccctg gccgccgacg tctccaaggg gctgaagacg
     3121 aagggcggca gccgggtgga ggcgggggcc acgcgcaacc tgctcaccgg ggtggacgac
     3181 gttatcgcga agctgcggcc cagcatcgag cgggccaggc agtcggcggt ggaactgttc
     3241 acgcagctaa actcggtgat ggaagtgtac caggacgagc agaacgtgta cgacctgcgg
     3301 cagcgcatca ccaacgaggt gcggcggcac gccacctggg ggcaggtgga gatgatctgg
     3361 gacaacctgg ggctgcaact gaaggatatc gaggacgggc tgtcgcgcat cggcaccgtg
     3421 ctgggcgacg tggactggga caacatcccc cgctccggca ccggagacgt gcccgcctac
     3481 gccgacatgc tgatcgagct gcggggcctc atcaccaacg tgcacaagtt gcgggcggac
     3541 gccaacgccg ggattaccaa cccggacgag ggggcggtct actggctgga ggcgcgggtg
     3601 aagggcggcg acgtggcgct gaggtgcgcg ccgctgcacg tgggggagct gctggaccgc
     3661 cacctcttct cgaagaagcg ggcggtggtg ctcacctcgg ccaccctctc gaccgacaac
     3721 gacttctcgt acatcttgga gcggctgggg ctgcacagtt gcgagcagtt gcagctatcg
     3781 tcgccgttcg actaccagaa ggccgcgctg gtgtacctgc cgaccgacat gccggagcct
     3841 tccacgccgg gctaccagcg cagcctcgaa caggcccttg tggagttatg taaagcatcg
     3901 caagggcgga cgctggcgct gttcacctcg cacagcgcgg tgcgggcgac gtacagggcg
     3961 atacagcgtc cattggaaga ggcgggcatc atggtgttgg ggcacaacat cgacggctcg
     4021 cggaagcagt tgcttgagcg gttcaagaac aacccgcgca cggtactgct gggcacgtcg
     4081 tccttctggg aggggataga cgtggtgggg gacgcgctgt cggtgctggt gatcgccaag
     4141 ctgccgttcg cggtgccgac cgacccggtg ttcgcggcgc gggccgaggg gttcgaggat
     4201 gcgtttttgc agtactcggt gccccaggcc atactgaagt tcacgcaggg ctttggccgc
     4261 ctcatcagga gccgcaacga ccggggagtg gtggccgtgc tcgaccgccg cgtcatctcc
     4321 aagcggtacg gccaggcgtt cctcaactcc ctccccgaag cgacggtcag acacgggctg
     4381 acagcgaacc tgccggggga ggtggcggac tggctagggc ataagtggta ggctgtgact
     4441 tccctcccgt cgttagtaca gctataatgt ggctatgcac taattgaagt gttccaaagc
     4501 attgctctag g
//
LOCUS       DASRWZ010000450         8046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_996772, whole genome shotgun sequence.
ACCESSION   DASRWZ010000450 DASRWZ010000000
VERSION     DASRWZ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996772"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VFH60_06990"
     CDS             <1..1119
                     /locus_tag="VFH60_06990"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6313564.1"
                     /translation="PAECLAEPSVTVAVSGQGEETFVEVVERFAAGSDLSGVKGVTWR
                     DAEGKIITNLPRPTADVNRFPRHNYDLIDVEAYFRHKGKRQLDYISSVGCRFRCAFCA
                     DPFVYNRSWYGLHAARIAEELSELSHKYSIEEVAFQDETFFTHRQRVEDICRAFLDAG
                     LRFTWTGTMRADQGHRLPDETLTLCRQAGLRRVMIGVEAGTDEMLKRIKKDITVAQVF
                     TTAEKLARHRIGAIWNFIVGFPDEPDDSFEATLDIAKKLRGMSKDFEAAVFFYKPYPG
                     NELADQLRSTNYAFPCSLEEWADFDYVGSAGPWVSREKFERVERFKFYQKYAFGRHSH
                     PITLPLQALARWRVDRDFYRFPIEQRLVEALHPAPRLS"
     gene            1138..2580
                     /locus_tag="VFH60_06995"
     CDS             1138..2580
                     /locus_tag="VFH60_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6313565.1"
                     /translation="MDILLTHGYFLYEDPHELKVMKPYPTLGLLYISSHLKSKGFAVE
                     LFDTTFSTLDAWKAYVTQNRPPIVGIYTNLMTKLNVLPMIRHCKAIGAHVVVGGPEPP
                     HYADQFLDAGADVVAVGEGELTLEALIPTLTASGPHRLHDVQGLVFRDEAGQTVRTPA
                     RPQIKNLDQQPFPDRDAIDMQRYLGTWKEHHGRSSVSLITARGCPYTCRWCSHTVFGN
                     THRRRSPENVVSELESIIERYRPDQLWYADDVLTIHRSWTLKFAALLKQRGIKVPFEC
                     ISRADRLDVEVIAALSEMGCSRLWIGSESGSQRVLDAMDRKTRVEDVQRKTHILREAG
                     IETGMFIMMGYEGEQIEDLEATVDHLKKSDPDLFLTTVAYPIKGTPYYNEVESRVLES
                     MPWHERTDRDLTVAGRRSKRFYSFATRWMVNEVGLHKMLKSRPRNVRRIARAFINSRI
                     GRIGMALSMGEKELAPAGRGQLHTENISGS"
     gene            complement(2601..3539)
                     /locus_tag="VFH60_07000"
     CDS             complement(2601..3539)
                     /locus_tag="VFH60_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HET6313566.1"
                     /translation="MPQGDAQQPSVLDYPAGQLHLEYTLSNGQMFRWRKTDDGWWDAV
                     SGSRMLRIRQVQGSQPDTDRFEYYTYPEPEDLAFVRRFLRLDVALPPLYESWRDADPY
                     LGSLGERFSGLRLVLQRPEECLLSFMCSTANFIPRIMKAIALIAREHGPPIEGPDGKV
                     LTHAFPGAEVIATLDPNALAEKTGLEWRAGNLVKVARQVASKPEGWLDEVARLDYATA
                     RGELMRMEGVGPKIADCVCLFAMSKDQAVPVDTHVWQLTRDRYLPALRGKTLTPTAYG
                     QVLQFFQTRFDKAGWAQQYLFYDHLLESRAKRGNRP"
     gene            3694..4011
                     /locus_tag="VFH60_07005"
     CDS             3694..4011
                     /locus_tag="VFH60_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6313567.1"
                     /translation="MAVRSNHIRVVELRELLNRERYEALDVRDPIAIARAAERFNVLD
                     AALSEFPSEEVLDLYRPLLSVSQAAKLLGYKPKEVRRLLGQGKISGKKQGNEWRIPLK
                     AVL"
     gene            4025..5230
                     /locus_tag="VFH60_07010"
     CDS             4025..5230
                     /locus_tag="VFH60_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6313568.1"
                     /translation="MAVDVAGAASSRESGAKFVSSRIEAVPPSGIRRFFDIAATMSDV
                     ISLGIGEPDFVTPEPIVKAGIDSLKAGHTSYTSNSGILELRQRLSGQLNKLYDLEFDP
                     ETELLVTVGVSEALMLALMAVLEAGDEVIVPEPCFVAYQPTVTFAGGKPVPVPTYVEQ
                     DFQVTVDDLEKARTRKTRAVLLGYPNNPTGAVLERSRLAEIAEWAERNDLLVVSDEIY
                     DRLVYGIEHTCFAALEGMRERTVLLGGFSKSYAMTGWRIGYAAAPPAILAAMRKIHQY
                     IIMSAPTTGQEAAIEALRSGEPFVEAMREEYDLRRRTIVDGFNRMGLDCFEPRGAFYA
                     FPSIKSTGLSDADFSERLLMEHKVAMVPGSAFGESGAGHVRASYATSLPNIEEALDRI
                     DRFLSVLKK"
     gene            complement(5358..6428)
                     /gene="holB"
                     /locus_tag="VFH60_07015"
     CDS             complement(5358..6428)
                     /gene="holB"
                     /locus_tag="VFH60_07015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET6313569.1"
                     /translation="MHNLPPQPSGWPVVGQDQPVGILRRSLRTGRLSHAYLFTGPDGI
                     GKRTVALALAMTVNCLGETPPGQPWPDVPCLLCPSCARIMRGAHPDVSEVNLEVQAQQ
                     QGDSGKKGAPSRELRIDVVRDMQATLGLNPHSARRKVYLLGDADRLNEEASNCLLKTL
                     EEPPEHSMLVLMAPDEESVLPTISSRCVQLQFRPLSRATVASSLASIWGAEEEQAETL
                     AALSGGRLGYAVGLLGNDQALARRRTALEEAALLTGAPVLDRVEAAARYAKRYTDARS
                     ELFDMLDEWETWWRDVMVVKVGAGDLAVSVDQLPSLNSIARRTPIAKAADAVRLIQQT
                     RKQLLENVNPRLALEALTLGLP"
     gene            complement(6445..7245)
                     /locus_tag="VFH60_07020"
     CDS             complement(6445..7245)
                     /locus_tag="VFH60_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HET6313570.1"
                     /translation="MNVFTHDSTDLQAARRRARLMQYGGLLAIVGSVLSSLFFPFYEA
                     LVFFAYPLLFAGLPLWTIGRNRQRQLVNAPKIKEMITSELKGLSDKYSLHHGPSVGGR
                     RIGHLLITPSGIIVIAANEATGPITCTSDGKGDRWQTRMTLMDRFSGTRQPIGNPTLE
                     LDAATAAVTALLAKQDKEDVPVRGLVVFVANPDIEIEASSYPAVPLNELRLAVRELQT
                     IMADEREEGLDAERLLTSEDRKRLNVALGPATVTKATQATTRPASARS"
     gene            7422..>8046
                     /locus_tag="VFH60_07025"
     CDS             7422..>8046
                     /locus_tag="VFH60_07025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6313571.1"
                     /translation="MSIVVVSWNTRELLEQCLRSCSQPSVTGNLRTEVIVVDNASGDG
                     SAGAARTFAGVEVVALSHNIGYGRANNLGIARGSGRYYLILNPDTILLPGSVEGLVAY
                     ADGAPRAGIVAPRLLNEDLTVQTSAFKFPTLGMTAIDLFPLPNWLPGRVRIRLLNSAL
                     NGRYAIEQQANEPFLIQHPLGACMLIRREAYRACGGFDPNIFMYSEEI"
BASE COUNT         1473 a         2593 c         2522 g         1458 t
ORIGIN      
        1 cccgctgagt gcctggcgga accaagcgtg actgtggccg tcagcggcca gggcgaagag
       61 acgttcgtgg aggtcgtgga gcggtttgcc gcgggcagcg acttgagcgg cgtaaagggc
      121 gtcacctggc gcgacgcgga aggcaagatc atcacaaatc tgcctcgccc cacggcagac
      181 gtgaaccgct tcccccggca caactacgac ctgatcgacg ttgaagccta cttccggcac
      241 aagggtaagc ggcagcttga ctacatctcc tcggtgggct gtcgcttccg ctgcgccttc
      301 tgcgccgacc cgttcgtcta caaccggagc tggtacggcc tgcacgccgc ccgcatcgcg
      361 gaggagcttt cagagctgtc gcacaagtac agcatcgagg aggtggcctt ccaggacgag
      421 acgttcttca cccaccgcca gcgcgtcgag gacatctgcc gcgccttcct cgatgccggg
      481 ctgcgcttca cctggaccgg caccatgcgc gccgaccagg gccaccgcct ccccgatgag
      541 accctcactc tctgccgtca ggcggggctg cgccgcgtga tgatcggcgt ggaggcgggc
      601 accgacgaga tgctgaagcg catcaagaag gacatcacgg tggcccaggt tttcaccacc
      661 gcagagaagt tggcccggca ccgcataggc gctatctgga acttcatcgt cggcttcccg
      721 gacgagccgg atgacagctt cgaggccacc ctcgacatcg ccaagaagct gcgcggcatg
      781 agcaaggact tcgaggcagc cgtgttcttc tacaagccct accccggcaa cgagctagcc
      841 gaccaactgc ggtccaccaa ctacgccttc ccctgctcgc tggaggagtg ggccgacttc
      901 gattacgtag gctccgcggg gccgtgggtg agccgcgaaa agttcgagcg ggtggagcgg
      961 ttcaagttct accagaagta cgcatttgga cggcacagcc atcctatcac cctgccgcta
     1021 caggcgcttg cccgctggcg agtggaccgc gatttctacc gctttcctat agagcagcgt
     1081 ctggtcgagg cgcttcaccc cgcacccagg ctatcctaga caaggcccaa aggcattatg
     1141 gacattctcc tgacccacgg ctatttcctc tacgaagacc ctcacgaatt gaaggtcatg
     1201 aagccgtacc ccaccctcgg cctcctctac atctcctcac acctaaagag caagggcttc
     1261 gccgtcgagt tgttcgatac taccttctcc acactggacg cctggaaagc ttacgttacg
     1321 cagaaccggc cccctatcgt cggcatctac acgaacctga tgaccaagct gaacgtactg
     1381 cccatgattc gccattgcaa ggctatcgga gcgcacgtcg tagtcggcgg cccggaaccg
     1441 ccgcactacg cagaccagtt tctggacgcg ggggccgacg tagttgcggt gggcgagggc
     1501 gagcttacgc tagaagccct catcccgact ctaaccgcga gcggcccgca ccggctgcac
     1561 gacgtgcagg ggctggtctt ccgggatgag gcggggcaga cggtgcgtac tcccgcccgc
     1621 ccccagatca agaatctgga ccagcagccc ttccccgacc gcgacgcgat cgacatgcag
     1681 cgttacctgg gcacctggaa ggagcaccac ggtcgtagct cggtgtcgct gattaccgcc
     1741 aggggttgcc cctacacctg ccgctggtgc agccacaccg ttttcggcaa cacccaccgc
     1801 aggcgctcgc ccgaaaacgt cgtgtcggag ctagagtcaa tcatcgaacg ctaccggccc
     1861 gaccagctct ggtacgccga cgacgtgctc accattcacc gttcttggac cctcaagttc
     1921 gcggctcttc tcaagcagcg gggcatcaag gtacccttcg agtgcatttc ccgcgccgac
     1981 aggctagacg tggaggtgat tgccgcgctg tccgagatgg gctgttcccg gctgtggatc
     2041 ggctcggaga gcggcagcca gcgcgtgctc gacgcgatgg accgcaagac gcgcgtggag
     2101 gacgtgcagc gcaagaccca tatcttgcgt gaggcgggca tcgagacggg catgttcatc
     2161 atgatgggct acgagggcga gcagatagaa gacctcgaag ccaccgtgga ccacctcaag
     2221 aaaagcgacc cggacctgtt cctcaccacc gtcgcctacc cgatcaaggg cacaccctac
     2281 tacaacgagg tcgagagccg ggtgctggag tcgatgccct ggcacgagcg caccgaccgc
     2341 gaccttaccg tcgcgggccg ccgttcgaag cgtttctact ccttcgccac gcgctggatg
     2401 gtaaacgagg tagggctgca caaaatgctc aagtcgaggc cgcgcaacgt gcgcaggata
     2461 gcgcgggcct tcatcaactc gcgcatcggg cgaatcggca tggccctgag catgggggaa
     2521 aaggagcttg caccggcggg gcggggtcaa ctgcacacgg aaaacatctc cggcagctag
     2581 ccctcatcac cgctgtgtgg ctagggccgg ttgcccctct ttgcgcggct ctccagcagg
     2641 tggtcgtaga agaggtactg ctgcgcccag cctgccttgt cgaaacgagt ctggaagaac
     2701 tgtagcacct ggccgtaggc cgtaggcgta agggttttac cccgaagcgc aggcaggtac
     2761 ctgtcccgag taagctgcca cacgtgcgtg tccaccggca ccgcctggtc tttagacatc
     2821 gcgaacaggc acacacagtc tgctatcttc ggccccacgc cctccatgcg cattagctcg
     2881 cctctggccg tggcgtaatc gagcctggcc acctcatcga gccagccttc cggcttcgat
     2941 gccacctgcc gggcaacctt cacaaggttc cctgcccgcc actcaagccc cgtcttttcg
     3001 gcaagcgcat tcgggtcgag ggtcgcaatt acctccgcgc cggggaaggc gtgagttagt
     3061 accttgccgt ccggcccctc gattggcggc ccgtgctccc gcgcaatgag cgcgatggcc
     3121 ttcatgatgc gagggatgaa gttggccgtt gagcacatga acgatagcag gcattcttcg
     3181 ggacgttgca aaacgagccg cagcccgctg aaccgctcgc caaggctccc caggtagggg
     3241 tcggcgtccc tccagctttc gtagaggggc ggtagcgcta cgtccagccg gaggaagcgc
     3301 cgcacgaacg ccaggtcctc cggttcgggg taggtatagt actcgaagcg gtcggtgtcg
     3361 ggttgggagc cttgcacctg gcggatgcgg agcatacggg agccgcttac cgcgtcccac
     3421 cagccgtcgt ccgtcttgcg ccagcggaac atctgtccgt tgctgagggt gtattcaagg
     3481 tgcaactggc ccgccgggta atccagcacc gaaggttgtt gagcgtcgcc ctgtggcatg
     3541 gctgtctcct cacccgcagt tataacatag cccccgcgac gagtcgaacc ttgcgccgcc
     3601 gtttaggaag cgcccgcgta tcgtgttata atgttcagta gtctcttaca agccgcagtc
     3661 cttcacaccg ggctgcgcac cggagaaacc gagttggcag tccgttcgaa ccacatccgc
     3721 gtcgtggagt tacgagagct tttgaaccgg gagcggtacg aggcgctgga cgtgcgcgac
     3781 cctatcgcca tcgctcgcgc ggccgagcgg ttcaacgtgc tcgacgccgc gctgagcgag
     3841 ttcccttccg aagaggtgct cgacctgtac cggcctctgt tgtcggtgtc gcaggccgcg
     3901 aaattactcg gctacaagcc caaagaggtg cggcggctgc tcggccaggg caaaatatcg
     3961 ggaaagaagc agggcaacga gtggcgtata ccgctcaaag cggtgctgta gaggaggatt
     4021 cataatggca gttgacgttg ccggtgcggc ctcaagccgc gagtccgggg caaagttcgt
     4081 gtcgagccgc atagaggccg tgccgccttc cggcatacgt cgcttcttcg acatagccgc
     4141 gacgatgtcc gacgttatct cgctcggcat cggagagccg gactttgtca cgcccgaacc
     4201 gattgtgaag gccggtatcg actcactcaa ggcggggcat accagctaca cctcgaactc
     4261 cggcatactg gagctaaggc agcgactctc aggccagctc aacaagcttt acgacctcga
     4321 attcgacccc gaaaccgagt tgctggtgac ggtaggcgtg agcgaggcgc tgatgctggc
     4381 cctcatggcg gtgctggaag cgggcgacga ggtgattgtg cctgagcctt gcttcgtagc
     4441 gtaccagccg acggtgacct tcgccggggg caagcccgta cccgtcccta cttacgtcga
     4501 gcaggacttc caggtgaccg tagatgacct tgagaaggcg cgcaccagga agacgcgggc
     4561 ggtgctcctc ggatacccga acaaccccac aggggccgtc ctggagcgtt ccaggctggc
     4621 tgagatcgcg gagtgggccg agcgcaacga cctgctggtg gtctcggacg agatttacga
     4681 tcgcctggtc tacggcatcg agcacacctg tttcgccgcg ttggaaggca tgagggagcg
     4741 cacggtcctg ctgggcggct tctccaagtc gtacgctatg acgggttgga ggatcggtta
     4801 cgccgccgcg ccccctgcta tactcgccgc catgcgcaag attcaccagt acatcatcat
     4861 gtccgccccc acgaccggcc aggaggcggc catcgaagcc cttcgcagcg gagagccttt
     4921 cgtggaggcc atgcgcgaag agtacgacct gcggcggcgc accatcgttg acggcttcaa
     4981 ccgcatgggc ctagactgct tcgagccgcg tggagccttc tacgccttcc cctccatcaa
     5041 gagtaccggc ctctccgacg ctgacttctc cgagaggctg ctaatggagc acaaggtggc
     5101 tatggtgcca ggctccgctt ttggggagtc gggcgcgggc cacgtgcgtg cctcctacgc
     5161 gacctcgctg ccaaacatcg aggaggcgct ggaccgcatc gacaggttcc tctccgtgtt
     5221 gaagaagtaa ttcctgcccc gcagataaat cggcgcgatt aagaagaagt ccccaggtgg
     5281 ggacttcttc gttttggacc attctcgcag gcgaaatgcc tagatgttgc ggctgcggtt
     5341 gaaatcgccc cgtgctctca gggcagcccc aacgtcaggg cctctagcgc cagcctgggg
     5401 ttgacattct ccagtagctg cttgcgggtc tgctgtatga gccggaccgc atccgcggct
     5461 ttcgcgattg gcgtgcggcg ggcaatggaa ttgagcgacg gtagttggtc cacgctgacg
     5521 gcgaggtccc ccgcgcccac cttcaccacc atcacgtcgc gccaccaggt ctcccactcg
     5581 tccaacatat cgaaaagctc ggagcgagcg tcggtgtagc gcttggcata cctggcggcc
     5641 gcctccaccc tgtcgaggac cggcgcgccc gtgagcagag ccgcctcttc gagggccgtg
     5701 cgccgcctcg cgagcgcctg gtcgttgccc agcagtccca ccgcgtagcc gagccttccg
     5761 cccgaaagcg ccgccagcgt ctccgcctgc tcctcttctg cgccccagat agaggcgagg
     5821 ctagaggcca cggtggctct tgagagcggg cgaaactgaa gctgcacgca ccgtgaggat
     5881 atggtcggca acaccgactc ctcgtccggg gccatcagca ccagcatgga gtgctcggga
     5941 ggttcctcca gcgtcttaag caggcagttg gacgcctcct cgttcagccg gtcggcgtcg
     6001 cctagcaggt acaccttgcg ccgcgcgctg tgcgggttca gccctagcgt ggcctgcatg
     6061 tcgcgcacca cgtctatcct tagctccctc gaaggtgcgc ccttcttgcc ggaatctccc
     6121 tgttgttgcg cctgcacctc caggttgacc tcggacacgt cgggatgcgc ccctcgcatg
     6181 atgcgggcgc aggaggggca aaggaggcaa ggcacgtcgg gccagggctg ccccggcggc
     6241 gtctccccca ggcagttcac ggtcatggca agggccaggg ctaccgtgcg cttgccgatt
     6301 ccgtccggcc cggtgaacag ataggcatgg gagagcctgc cggtcctgag agagcggcgc
     6361 aggataccta ccggctggtc ctgcccaacc acgggccaac ctgagggctg cggcgggagg
     6421 ttatgcatcg ctcttgcgct ctctctagct gcgtgccgag gcggggcggg tggttgcctg
     6481 agtggctttg gtcacggtcg cggggccgag agcgacgttc aggcgcttgc ggtcttcgct
     6541 ggtgagcagc cgctcggcgt caagtccctc ctcgcgctcg tccgccatga tggtctgcaa
     6601 ctcgcgcacg gccagcctga gttcgttcag gggcacggcc gggtacgaag aggcctcgat
     6661 ctcgatatcc gggttggcga cgaagaccac cagcccgcgc accggcacgt cctccttgtc
     6721 ctgcttggcg agcaaggccg taaccgccgc cgtagccgcg tcaagctcca gggtggggtt
     6781 gccgataggc tggcgggtgc cggagaagcg gtccatcagc gtcatgcgtg tctgccagcg
     6841 gtcgcccttg ccgtcgctgg tgcaggttat agggccggtc gcctcgttcg ccgcgatgac
     6901 gatgatacct gaaggggtaa tcagcaggtg cccgatcctt cgccctccca ccgaggggcc
     6961 gtggtggagg ctatacttgt cgctcaagcc ctttagctcg ctcgtaatca tctccttgat
     7021 tttgggggcg tttacgagct gccgctgccg gttgcgccct atcgtccaga gcggcaagcc
     7081 ggcgaacagc agcgggtagg cgaagaacac cagcgcctcg taaaaaggga agaaaagcga
     7141 gctgagcacg gagccaacaa tggctagcaa gccgccgtat tgcatgagcc gggcgcgtcg
     7201 ccgggcggct tgcaggtcgg tactatcgtg ggtgaaaacg ttcatacgtg tgcgatttct
     7261 cctcgcgtcg ggtaccgctt ccaacctcct gagagagggg cgagcggctg cacgccggag
     7321 ttgtccgctg gcggacggca tgctaaactg ggccggtgac taagtctaac acaaggtcgg
     7381 gggccgttca agagcaggca ccacctggcg caggccccga cgtgagcatc gtagtcgtga
     7441 gctggaacac tcgcgagctc ctggagcagt gcttgaggtc gtgctcccag ccgagcgtaa
     7501 cgggtaactt acgcacggag gtcattgtgg tagacaacgc ctccggagac ggcagcgcgg
     7561 gggcggcccg caccttcgcc ggggtggagg tggtagcgct ctcgcacaac atcggctacg
     7621 gccgggccaa caacctgggc atcgcgcgtg gttcggggcg ctactacctc atcttgaacc
     7681 ccgacactat cctgctacct gggtcggtcg aggggctggt ggcctacgct gacggcgcac
     7741 cgcgtgcggg catagttgcc ccacgcctct tgaacgaaga ccttaccgtg cagacaagcg
     7801 cgttcaagtt ccccaccctt ggcatgaccg cgatagacct gttcccacta ccgaactggc
     7861 tgccgggaag ggtccgcatt cgcctgctca acagcgctct caatggccgt tatgcaattg
     7921 agcaacaagc caatgagcca tttttgatcc aacatccgct cggcgcgtgc atgctgatca
     7981 gacgcgaagc ttaccgagcg tgcggaggct tcgaccccaa catcttcatg tattcggaag
     8041 aaatcg
//
LOCUS       DASRWZ010000451         3827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1271469, whole genome shotgun sequence.
ACCESSION   DASRWZ010000451 DASRWZ010000000
VERSION     DASRWZ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271469"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1637)
                     /locus_tag="VFH60_07030"
     CDS             complement(36..1637)
                     /locus_tag="VFH60_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HET6313572.1"
                     /translation="MIDGNQIAGVGRASAQPLLAIWEFAADAMVLSDLEGTVIAANPA
                     YCRLYGYSQEEIVGQSFAIIFPEELRAWAMQGYKDTFAQEDDLRLPVESEIQRKDGSK
                     GVVEVRYTFIMEDGRRTAMLSQIRDVTENKQAQAHLEAERARVATILEATTEGIYGMD
                     REGRCTFINSAALSFLGYTAEECLGRNMHELVHYQHLDGTPYPIEQCPIYGVLQDGVP
                     TRLLDETLWHKDGTPRPALYSCSPIMDEGRVGGGVVTIVDITERRKGEEERTQLLAQE
                     RAARVLAERAVRAQEELLSVVSHDLSNPISVIKGVTQLLQRRLARDNAPEPIHLAAEL
                     TKIASAATRIENFIRDLASPEHLQPGQSLSITPQCVDLVDLARRVAESHQLQTERHHL
                     VVEAESEELTGYWDPARLEQVLDNLVSNAVKYSPQGGVVRIGVGYQRSAGGDTDDSSR
                     AVVTVRDEGLGIPEKDINHVFEWYRRGTNVRSVIGGTGVGLAGARQIVEQHGGRIEVE
                     SREGEGSMFTVLLPVGGEATPPAEG"
     gene            complement(1699..2439)
                     /locus_tag="VFH60_07035"
     CDS             complement(1699..2439)
                     /locus_tag="VFH60_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET6313573.1"
                     /translation="MSDKAIPIFVGCAGWSIPKAFGERFGEGGSHLERYARLFPCVEI
                     NSSFYRPHKPATYDRWAASVPDGFRFAVKVPRTITHTRRLRDAGAELRDFLDECGHLG
                     SRLGPLLVQLPPSLAFHEQTVAAFLATLRAYFTGDVACEPRHPTWFAPGPDARLSEFQ
                     VARVAADPAVAPGAEEPGGWDGLAYYRLHGSPRIYYSAYPQDYLEAIARKLVGSAAGG
                     AQVWCIFDNTAEGAATGDALTVTDLVRG"
     gene            2660..>3827
                     /locus_tag="VFH60_07040"
     CDS             2660..>3827
                     /locus_tag="VFH60_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313574.1"
                     /translation="MKRLFVALAALVLVLIVARVPVGELFGTNGAAAQASAPAPQISL
                     KSPQTRQAVSFDVSPPLRSIPAAPVVPSTKQEGERENWMKPQNKVPNFVNVDPVVQHA
                     FGADAMPTPILTFKGYTQQDNAGTGPLIVHPPDTEGDVGLNHYFQWNNIGFKIFDKAG
                     NLLYGPVPGNTLFTGMTGPGGAACSGTNSGDPIVLFDTMANRWLASQFTTATLPFGPT
                     YECIAISTTDDPTGPYYRYAFQASPADTPTYFEDYPHFGVWPDAYYMATNEFNVSEDI
                     ASFVGAGNFAFERAAMLAGNPAAKMVYFHLPPPYGGFLPSDMDGPTPPPAGSPNYFIA
                     VDDNFGSPPADQLSMFKFHVDWTTVASSTFTGPITTTVAAFDADLCAAEREACIP"
BASE COUNT          655 a         1319 c         1099 g          754 t
ORIGIN      
        1 tcctgtgacc aacacggcaa gggactcgct cggcctcacc cctcagcagg aggggtcgcc
       61 tccccaccca cgggcagcag caccgtgaac atagaccctt ccccctcgcg gctctccacc
      121 tcaatccgcc cgccgtgttg ctctactatc tggcgcgccc ccgcaagccc tacccccgtc
      181 ccgccgatca ccgacctgac gttggtgccc ctgcggtacc actcgaatac gtggtttatg
      241 tccttctccg gtatgccgag gccctcgtcc cgcacggtca ccaccgctcg cgagctgtcg
      301 tctgtgtcgc cgcctgcgct tcgttggtag ccgacgccga tccgcaccac gccaccctgc
      361 gggctgtact tcaccgcgtt cgatacgagg ttgtcgagca cctgctccag gcgggcgggg
      421 tcccaatagc cggttagctc ctcgctttcc gcctccacca ccaggtggtg acgctccgtc
      481 tgcaactggt gagactcggc cacgcgccgc gccaggtcca cgaggtccac gcactggggt
      541 gtgatgctca gcgactgccc cggctgtagg tgctcaggac tcgccaggtc gcgtatgaaa
      601 ttctcaatcc gggtcgcggc gctggcgatc ttggtcagtt cggccgccag gtggataggt
      661 tcgggggcgt tatccctcgc cagccgcctt tggagtagtt gcgtcacgcc ctttatcacc
      721 gatatggggt tgctcaggtc gtgcgacacc acggagagca gttcctcctg ggcacgcacc
      781 gcccgctcgg ccagcactct cgccgcccgc tcctgagcca gcagttgcgt ccgctcctcc
      841 tcgcctttcc tgcgctccgt tatatccact atcgtgacca cgccgccccc caccctgcct
      901 tcgtccatta tgggagagca cgagtagagc gcgggccggg gcgtgccgtc cttgtgccag
      961 agggtctcgt ccagcagccg cgtgggcacg ccgtcctgta gcacgccata aatggggcac
     1021 tgctcgatag ggtaaggggt gccgtcgagg tgctggtagt gcacaagctc gtgcatgttg
     1081 cgccccaggc attcctcggc cgtgtacccg aggaagctta gcgcggcgga gtttatgaag
     1141 gtgcacctgc cttccctgtc catgccgtat atgccctcgg tggtcgcctc caatatggtg
     1201 gctacgcgcg cccgttccgc ttcgaggtgc gcctgggcct gcttgttctc cgtcacgtcc
     1261 ctgatctggg agagcatggc cgtgcgccgc ccatcttcca tgatgaacgt gtacctgact
     1321 tcgactacgc ccttgctgcc gtctttgcgc tgtatctcag actctacggg caggcgtaag
     1381 tcgtcctcct gcgcgaacgt atctttgtac ccttgcatcg cccaggcccg cagctcttcg
     1441 ggaaagataa tcgcgaaact ctgcccgacg atctcctctt gcgagtaccc gtacaggcgg
     1501 caatatgcgg ggttcgcggc tattacggtg ccctcaaggt cggacagcac catagcgtcg
     1561 gcggcgaatt cccagatggc aagcagcggc tgtgcgctgg ccctccctac accggcgatc
     1621 tgattcccgt caatcatgcc cctactatac cacaggcacc aatggcggaa gcatcggtgg
     1681 ctgggggcgt tcgatgccct aacccctcac aagatccgtc acggtcagcg catcccccgt
     1741 ggcggcacct tccgcggtgt tgtcgaatat gcaccatacc tgcgcgccac ctgccgcaga
     1801 ccccaccagc ttccgcgcga tagcttccag gtagtcttga gggtaggccg agtaataaat
     1861 gcggggcgag ccgtgcagcc tgtaatatgc caggccgtcc cacccgccag gctcctccgc
     1921 gcccggcgct acggcggggt cggcagccac gcgggccact tggaactcgg agaggcgcgc
     1981 gtcaggccca ggggcgaacc acgtggggtg gcgcggctcg caagctacgt cccctgtgaa
     2041 gtacgcgcgc aacgtggcga ggaaggccgc taccgtctgc tcgtggaagg ccaggctggg
     2101 gggtagctgc accagcagcg ggcctagcct ggagccgaga tgcccgcact cgtccaggaa
     2161 gtcgcgaagc tcggcaccgg cgtctcgcag gcggcgcgtg tgggtgatcg tccggggcac
     2221 tttgacggcg aagcggaagc cgtccggcac ggaggcggcc cacctgtcgt aagtggcggg
     2281 cttgtgcggc cggtagaacg aactgttgat ttccacgcaa gggaacaggc gcgcgtacct
     2341 ctcaaggtgg ctgcctccct cgccaaatcg ctccccgaac gctttcggga tgctccaacc
     2401 ggcgcagccc acgaatatcg gaattgcttt gtctgacatg agacccccca ccctcctgct
     2461 ccgcggtgcc acttgtgcgt cctacgaact ggggcacagt tcgtgctacc gcaggctgtt
     2521 tgccagttta atactgctac taccttcatg agtattagca ctctacaggc caccatgtac
     2581 gttcgacaat tcgccaagcc tggcatgtgc gggcctcgat gaggaggtcc gtgtttaata
     2641 cccgaaggag gacaggcgcg tgaaacgatt gtttgtagct ttggcagccc tcgtattggt
     2701 gctgattgtc gcacgcgtgc ccgtgggcga gctatttggc accaatgggg ccgcggccca
     2761 ggcaagtgcg cctgccccgc agatatccct caagtctccc caaacgcggc aggccgtcag
     2821 cttcgacgtg tcccctccgt tgcgttcgat tcctgccgcc cccgtcgtgc catctactaa
     2881 acaggagggg gagcgcgaga actggatgaa gccccagaac aaggtgccca actttgtcaa
     2941 cgttgacccg gtggtgcaac acgcgtttgg cgcggatgcc atgcctacac ctatcctcac
     3001 tttcaaaggc tacacccagc aggataacgc cggcaccggc ccgctgatcg tccacccgcc
     3061 tgataccgag ggagacgtgg gcctgaacca ttacttccag tggaacaaca taggctttaa
     3121 gatatttgac aaggccggca atctgctcta cggacctgtg cccggcaata ctctctttac
     3181 cggcatgacc gggccgggcg gggccgcgtg ctccggcacc aacagcggcg accccatagt
     3241 cctctttgac actatggcta atcgctggct ggccagccag ttcaccacgg cgacccttcc
     3301 cttcggccct acctatgagt gcatagccat ctctacgacc gatgacccga ccggccctta
     3361 ctatcgctac gctttccagg ccagccccgc cgatactcca acttacttcg aggactaccc
     3421 gcactttggg gtgtggcccg atgcctacta catggccacc aacgagttca acgtctccga
     3481 agacatcgct tcttttgtgg gagctggtaa cttcgcgttc gagcgggccg cgatgctggc
     3541 cggtaacccg gccgccaaaa tggtctactt ccacctgcca ccgccgtacg gaggtttcct
     3601 tccctccgac atggacggcc ccacaccccc acccgcgggc agccctaact acttcatagc
     3661 agtagatgat aacttcggca gccctccggc cgaccagctt tcgatgttca agttccacgt
     3721 cgattggacc acggtagcga gttccacctt caccggcccc attaccacca ctgtagcagc
     3781 gttcgacgcc gacctgtgcg ccgcggagcg agaagcctgt atcccac
//
LOCUS       DASRWZ010000452         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1202837, whole genome shotgun sequence.
ACCESSION   DASRWZ010000452 DASRWZ010000000
VERSION     DASRWZ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202837"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1090
                     /locus_tag="VFH60_07045"
     CDS             302..1090
                     /locus_tag="VFH60_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET6313575.1"
                     /translation="MNIVVCMKSVPDTTAEKRLLPDGTLDRASVPAVINPWDEYAIEE
                     ALRIRESGAATGEIVLLCMGPENSTETIRKGLAMGADRAVLVADPALHGSDMWATARV
                     LAAALKTLQFDIVMFGSQSTDAGGGIIYNAVAEMLGLPAVTWVNEVTVQGGKVRGKRG
                     SDVGYDVVEAPVPCVVSVTQTANEPRYPSLPGIMKAKKKEIASHNVASLGIDASEVGL
                     PGARTQVTGTERPQTARANQVLKAEDPATTAAAIADFLESKKHI"
     gene            1160..2155
                     /locus_tag="VFH60_07050"
     CDS             1160..2155
                     /locus_tag="VFH60_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET6313576.1"
                     /translation="MATNNDVWAIVETDSGGRPKRLGLELTSMAAQLAGQYGGQGGAV
                     VIGSREAAEAVGQYGASQVFYVSDERCKSDIVGPAAAAIAALIGQHNPRLVLLPSTPL
                     GKDWAGRICGKLGLGINADTVDVTVEGGKATAVTPAFNGSLRVSAQFKTEGEQTALAV
                     VRPGVATAKRAEGATSNVQEAPVPGDVTPGMTVVESVVEKGGVPDLAGAQVIVAGGRG
                     LGSAEKFDMVRELADSLGGAVGATRAIVDMGWIPYSYQIGQTGKTVRPKLYVAVGISG
                     EIQHKVGMQTSGTIVAINKDPNAPIVQFCDLAVVGDLHQVVPALVAEINRRKGTA"
BASE COUNT          423 a          659 c          769 g          366 t
ORIGIN      
        1 gggtcaagaa cgggcggccg atccttcgct acgctcagga tgacaatggg gatagttttc
       61 tgggcatcat agctcactcc gctaaccctc tttgttctct gagttacctc tgcgtctctg
      121 cgtctctgcg gtgaaaaaca ctcagcctct ttgcgcgttc ttggcgttcg tggcgtgcct
      181 ggcgtttccg tctcctggcg ttggggcatg gctcctgctt gaccatatgg gtggcttgtg
      241 atacgattca ccgaatgtcc ggcgaaatcg ccaatccgca tcgatatagg agacaacggc
      301 gttgaatata gtagtatgca tgaagtccgt gcccgatacc acggctgaga agagactcct
      361 gcccgacggc acgctcgacc gcgcttctgt gcccgcggtc atcaacccct gggacgagta
      421 tgccatagag gaggcgctac gcattcgcga gagcggcgcc gccacaggcg agatcgtcct
      481 cctgtgcatg gggccggaga acagcaccga gacgatccgc aagggcctcg ctatgggggc
      541 cgaccgcgcc gtgctggtgg ccgaccccgc actccacggc tccgacatgt gggccaccgc
      601 ccgcgtgctg gccgccgccc tcaagaccct gcaattcgac atagtgatgt tcggctcgca
      661 gagcaccgac gcgggaggcg gcatcatcta taacgcggtc gcggagatgc tgggcctgcc
      721 cgccgtcacg tgggtcaacg aggtgaccgt gcagggcggc aaggtgaggg gcaagcgcgg
      781 cagcgacgtc ggctacgacg tggtggaagc cccggtgccc tgcgtggtgt cggtgacgca
      841 gaccgccaac gagccgcgct acccgtcgct ccccggcatc atgaaggcca agaagaagga
      901 aatcgcgagt cacaacgtgg ctagcctcgg catcgacgcc tcggaggtag ggctgccagg
      961 cgcgcgcacc caggtcacgg gcaccgagcg cccgcagacc gcccgcgcca accaggtcct
     1021 caaggccgaa gacccggcca ctacggcggc agccatagcc gatttcctgg aaagcaaaaa
     1081 acacatctga ttttggatta tggattttag atttaaggag tgaatctaaa atccataatc
     1141 taaaatggta ggagggatcg tggcaacgaa caacgatgtt tgggcgatag tggagaccga
     1201 ctccggcggg cggcctaaga ggctggggct ggagcttacg agcatggcgg cgcagttggc
     1261 cgggcagtat ggcgggcagg gcggcgctgt cgttatcggg tcgcgagagg cggctgaggc
     1321 ggtggggcag tacggggcga gccaggtgtt ctacgtgagt gacgagcgtt gcaagagcga
     1381 catagtgggg cctgcggccg ctgccatcgc cgcgctcatc ggccagcaca accccaggct
     1441 ggtgctcctg ccttccacgc ctcttggcaa ggactgggcg gggcgcatct gcggaaagct
     1501 ggggctgggc atcaacgccg acaccgtaga cgtgaccgtg gaggggggca aggctacggc
     1561 cgttacgcct gccttcaacg gctctttgcg ggtgagcgcg cagttcaaga cggagggcga
     1621 gcagacggcc ctggcggtgg tgcgtcccgg cgtggcgacc gcgaagcgcg cggagggcgc
     1681 aacctccaac gtgcaggagg cccccgtgcc cggcgacgtg acccccggca tgacggtggt
     1741 ggagagcgtg gtcgagaaag gcggcgtgcc cgacctggcc ggggcgcagg tgatcgtggc
     1801 gggcggccgc ggcctgggca gcgccgagaa gttcgacatg gtgcgcgagc ttgccgactc
     1861 gctgggtggg gccgtgggcg ctacccgcgc cattgtggac atgggctgga taccctactc
     1921 gtaccagata gggcagaccg gtaagacggt gcggcccaag ctgtacgtgg cggtgggtat
     1981 ctccggtgaa atccagcaca aggtgggcat gcagacctcc ggcaccatcg tcgcgatcaa
     2041 caaggacccc aacgcaccaa tcgtccagtt ctgcgacctg gcggtggtgg gcgacctgca
     2101 ccaggtggtc ccggctctgg ttgccgaaat caaccggcgt aagggcacag catagcgggg
     2161 agacgaaacc gccaagacgc taaggacgcc aagacgccaa ggttaatgtg taagggc
//
LOCUS       DASRWZ010000453         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_791581, whole genome shotgun sequence.
ACCESSION   DASRWZ010000453 DASRWZ010000000
VERSION     DASRWZ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791581"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..509)
                     /locus_tag="VFH60_07055"
     CDS             complement(123..509)
                     /locus_tag="VFH60_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6313577.1"
                     /translation="MSNFNHPFVHIEFSSNDPAKSAEFYSSLFGWPMHEIKEFNYWTF
                     QTAEGQGGGFNKVGNDNGSLNVKPGDVVAYVDTDDIERDLAKAQELGGTIVAPKMEIP
                     GIGWYAIFRDPSDNLVGLYTNLPRPA"
     gene            741..1088
                     /locus_tag="VFH60_07060"
     CDS             741..1088
                     /locus_tag="VFH60_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET6313578.1"
                     /translation="MQSTAATVDEYLETVPPERLAALTRLRQLCLEKLQGYEESMQYG
                     GPAYSKNGVVEISFISQKNYISFYVLKNVVVDRYRDQLKDVGKGCIRYRRPDQIDFAV
                     VEKLLAETASSPA"
     gene            complement(1085..2266)
                     /gene="murG"
                     /locus_tag="VFH60_07065"
     CDS             complement(1085..2266)
                     /gene="murG"
                     /locus_tag="VFH60_07065"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET6313579.1"
                     /translation="MESTLPKSKIQNPKSKIALTGGGTGGHVSPAVAVITRLRERSAD
                     EGWGLDLLYVGSVAGVERRTITAMGVPYEAIQTGKLRRYLSPQTPLDLLVRLPAGFFQ
                     AFAALRRFKPNVIFSTGGYVCVPTVMAGRMLGVPSLTHEQTALVGLANRIAGRFADRV
                     AISFPRSASFFPPGKTLLTGNPVRPTIFQGDPSRAARLFGFDPVVPTLYVTGGAQGSH
                     AINMAVRDALPRLLACCQVVHQCGEGPDGSGDDLRALQLARETLPPDLRQRYHVLAFV
                     GEEIGDVYALASLVVGRAGAGTVNELASLGKPAVLVPLPGSAGGEQEANARALEKEGG
                     ATVLLQSNLTPDSLAGTVCDLLTDRARLGSMREGAGKLAMPGAADAIIDELLRLAKGN
                     S"
BASE COUNT          407 a          659 c          788 g          442 t
ORIGIN      
        1 gcaccgtttg acaacctgag ggggcactcg ccttgttctc tgcggtgaat acgtctgctg
       61 attgtggcaa ccaaaaggcc ctccggggta ggggagggcc ttgtgtgggg ctttgcgggt
      121 ggttacgcgg gccgtgggag gttggtgtag aggccgacga ggttgtcgga ggggtcgcgg
      181 aagatagcgt accagccgat gccgggtatc tccatcttgg gcgcgacgat ggtgccacct
      241 agctcctggg ccttggcaag gtcgcgctct atgtcgtcgg tgtcgacgta ggcgaccacg
      301 tcgcccggct tgacgttgag gctaccgttg tcgttgccga ctttgttgaa gccgccgccc
      361 tgaccttcgg cggtctggaa ggtccagtag ttgaattcct tgatctcgtg catgggccag
      421 ccgaagaggc ttgagtagaa ctcggcggac ttcgcggggt cgttggacga gaattcgatg
      481 tgaacaaagg ggtgattgaa attggacatg tgctttgctt gccctcctgg gtgatgggga
      541 attcactggg cgacgacgaa agcgggggcg atgtgtattg acagtataga acttttgttc
      601 tacaatgtca ataggtccgg gggagtagtt tgcacatcct gggcgagttg ttccaggatt
      661 ttggctgggg atgaaggatg gtaaagtggc ctatccgacc ctctcggtcc actctacttt
      721 tggcgaagga ggcgtgaact atgcaaagca cggcagccac cgttgacgaa tacctggaga
      781 ccgtaccgcc ggagcggctc gcggcgctca ccaggctccg gcagctatgc ctggagaagt
      841 tacagggcta cgaagagagc atgcagtacg gaggccctgc gtacagcaag aacggtgtgg
      901 tagagattag tttcatcagc cagaagaact acatctcgtt ctacgtactc aagaacgtcg
      961 tagtggacag gtaccgggac cagctgaagg acgtgggtaa aggctgcatc cgctaccgga
     1021 ggcccgacca gattgacttc gctgtggttg agaagttgct ggcggaaacc gcaagctcgc
     1081 ccgcctagct attccccttc gccaacctca acagctcgtc aataatcgcg tctgccgcac
     1141 ccggcatcgc cagcttcccc gccccttcgc gcatcgaccc gagtctggct ctatctgtaa
     1201 gcaggtcgca cactgtgccc gccagcgagt cgggggtgag gttactttgg agaagcacag
     1261 tggccccgcc ttctttttcg agggcgcggg cattggcttc ctgctcgccg ccagccgagc
     1321 cggggagggg caccagcact gcgggcttgc ccaggctcgc cagctcgttc accgtgcccg
     1381 cccccgcccg ccctaccacc agcgaggcca gcgcgtacac gtcgcctatc tcctcgccca
     1441 cgaacgccag gacgtgatag cgctggcgga ggtcgggtgg cagcgtctct cgggctagct
     1501 ggagggcgcg caggtcgtcg cctgagccgt ccggtccctc gccgcactgg tgcactacct
     1561 ggcagcaggc cagcagccgg ggtagggcat cgcgcaccgc catgttgatg gcatgcgagc
     1621 cttgcgcccc gcccgtgacg tagagcgtag gcacgaccgg gtcgaagccg aacagtcgcg
     1681 cggcgcggga ggggtcgccc tggaaaatgg tgggccgcac ggggttgccg gtaaggaggg
     1741 tcttaccggg cgggaagaag gaggccgagc gggggaagct gatcgctacc ctgtcggcga
     1801 agcggcccgc gatgcggttg gcgaggccca cgagggcggt ctgctcgtgg gtgagggagg
     1861 gcacgcccag catgcggccc gccatgacag tggggacgca cacgtagccg ccggtgctga
     1921 atatcacgtt gggcttgaag cggcgcaggg cggcaaatgc ctggaagaac ccggcgggca
     1981 gcctcacgag caggtcgagg ggcgtctgtg gcgacaggta gcggcgcagc ttgccggtct
     2041 gtatcgcctc gtagggcacg cccatagcgg tgatagtgcg cctctccacg cccgccacgc
     2101 tgccgacata gagcaggtcc agcccccaac cctcgtccgc gctcctctcc cgcagccgcg
     2161 taatcaccgc cacggcaggg ctgacgtgcc cgcccgtacc accgccggtg agggcgattt
     2221 tcgattttgg attttggatt ttggatttgg ggagtgtgga ttccaagttg gaaggtggtt
     2281 gcggcaaggt ttttta
//
LOCUS       DASRWZ010000454         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_106338, whole genome shotgun sequence.
ACCESSION   DASRWZ010000454 DASRWZ010000000
VERSION     DASRWZ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106338"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFH60_07070"
     CDS             <1..735
                     /locus_tag="VFH60_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HET6313580.1"
                     /translation="ADEAATIEGGDVLVIREDLVLVGLSERTSSEAIDDLAKGFAKKG
                     APVKTVYAVIMPKQRSTMHLDTIFTMMSHEDLLVYPPLILPGGGEQVGIVRIKVGRDG
                     TTQLQQVRKSLVDCLEEELGRPLNPVFCGGNSRLYQEREQWTDGANSFCLRPGLILGY
                     ERNEQTYRAMEAAGYRIISEDEIVKWNVAPGAARNWVVNQPLVDEIRQHPERKYAIKI
                     SGLELSRARGGPRCMTMPLVRDDIAW"
     gene            932..1777
                     /locus_tag="VFH60_07075"
     CDS             932..1777
                     /locus_tag="VFH60_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6313581.1"
                     /translation="MARDLFSQDPPPADHRIPYGPGEFHFADLRLPQNPQPALERSEG
                     SAIPNSKFPLVVFIHGGFWRSRYDLMHAGHAASALTEAGLATWSIEYRRLGNPGGGWP
                     GTLQDVGVAVDHLRTVAPRYNLDLERVLVAGHSAGGHLAAWVASRHRIPPGGPLYMED
                     PLPVKAAVPLAGVVDLRRGWELQLSSGVVEDFLGGPPDAMPERYALASPIEMLPTGVP
                     QRLVHGTDDPNVPYEISERYVRAAQERGDNVRLVTLPGAGHFEVINPDTKEWRQVVST
                     MLELI"
     gene            1853..2245
                     /locus_tag="VFH60_07080"
     CDS             1853..2245
                     /locus_tag="VFH60_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313582.1"
                     /translation="MQKMSKPVSMLWLALVIGDLAVVAYLHFAGGLAYTPVPDLPLLY
                     WVLLLLVPVLQLAPVVVLGVVPMPRGRSSEASLGVLLLSGGLSLLLALFNHLTQPEWG
                     GNTVLFAALGCISFASAYTMYRRMQASK"
     gene            2322..2864
                     /locus_tag="VFH60_07085"
     CDS             2322..2864
                     /locus_tag="VFH60_07085"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6313583.1"
                     /translation="MPEETWQVLGSEPVIEHPFLKVSVQQVRLPDGRVIPDWHIVHTR
                     DYVNVFVLNEAGQALILEGYKHGLGKSSWQVVGGYVEDEEDPLRTAQRELLEETGYAS
                     DDWRHLGSFVVDANRHVGKGHFFLARDARLVARPDHDDLEGFEVRWVSLEEVGAALRD
                     GRVGIISYGVNIALALLALR"
     gene            complement(2880..3950)
                     /locus_tag="VFH60_07090"
     CDS             complement(2880..3950)
                     /locus_tag="VFH60_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6313584.1"
                     /translation="MKGTILATALAAGAGVAVAANVTKKSKPYTFMDKSVVITGGSRG
                     LGLIMARLLADEGARVTILARTQEQLDRAVQDISVGGADVLAFRCDVTDRQQLENTLR
                     QVIEHRGRIDVLVNNAGVIQVGPVEHMQLQDFEDAMKVHLWAPLYAMQAVIPHMKREG
                     EGRIVNIASIGGKIAVPHLLPYVASKFALVGLSDGMRAELAKDNIHVTTVAPGLMRTG
                     SPYNALFKGDRKKEFGLFMLLGTVPLTAMSAQRAARQIIEACRNGSPELTTTIQARLG
                     ILASTLFPGLFARSLQLAVRLLPGPQGEPGDEARSGWESQSGFTRSPIMAPTQRAAEE
                     NNEHRGQHVAVDAEMEGADAGA"
BASE COUNT          738 a         1239 c         1371 g          781 t
ORIGIN      
        1 gccgacgaag cggctaccat agaaggcggc gacgtgctgg tgatacgcga ggacctggtg
       61 ctggtgggcc tgtcggagcg gacatcctcc gaggccatag acgacttagc aaagggattt
      121 gcaaagaaag gtgcgcccgt caagacggtt tacgccgtga ttatgcccaa gcagcgttcg
      181 accatgcacc tcgacaccat attcaccatg atgtcgcacg aggacctgct ggtctatccg
      241 ccgctcatcc tgccgggcgg cggcgagcag gtgggcatcg tgcgcatcaa ggtcgggcgg
      301 gatggcacga ctcagttaca gcaggtgagg aagtcactgg tggactgtct ggaggaggag
      361 cttggcaggc ctctcaaccc ggtcttctgc gggggcaaca gccgccttta ccaggagcgc
      421 gagcagtgga ccgacggggc caactcgttc tgcctgcgcc ccggcctcat actgggctac
      481 gagcgcaacg agcagaccta ccgggcgatg gaggcggcgg gctaccgcat catttcggag
      541 gacgagatcg tcaagtggaa cgtagcgccc ggtgcggcgc gcaactgggt ggtcaaccag
      601 cctctggtgg acgagataag gcagcacccg gagcgcaagt acgcgataaa gatcagcggc
      661 ctggagctat cccgcgccag gggcggcccc cgttgtatga cgatgccgct ggtgagggat
      721 gatattgcgt ggtgaggatg gacgatggac gatatgagac ggaaacgcaa agacgcaaag
      781 ggcgcaaagg acgcgcaaag aaaattaagg ttaggtgtgg cgggtagctg atgacttctc
      841 tatcgtccat cgtcccatct agctattgat gcctgcctta caattcttcc gcagaatgcg
      901 caaccaattt gccgccacga ggtcggcgct tatggctcgt gacctgttct cccaggaccc
      961 accccccgcc gaccaccgca tcccctacgg ccccggcgaa ttccacttcg cagacctgcg
     1021 cctcccccaa aatccgcaac ctgcccttga gcgaagcgaa gggtccgcaa tcccaaattc
     1081 caaattcccc ctagtcgtct tcatccacgg cgggttctgg cgatcgcggt acgacctgat
     1141 gcatgcggga cacgcagcga gcgcgctaac tgaggccggg cttgctacgt ggagcataga
     1201 gtaccggcgc ctgggcaatc cgggtggggg gtggccgggg acgctccagg acgtgggtgt
     1261 ggcggtggac cacttgcgga cggtagcgcc acggtacaac ctggacctcg agcgtgtgct
     1321 ggtggcgggg cactcggcgg gcgggcacct ggcggcctgg gtggcctcac ggcatcgcat
     1381 accgcccggc ggtccgctct acatggaaga cccgttgccg gtgaaggcgg ctgtgcccct
     1441 ggcgggcgtg gtggacttga ggcgcgggtg ggagttacag ttgagcagcg gggtggtgga
     1501 ggactttctt ggcggcccgc cggatgccat gccggagcga tatgccctgg cctcgcctat
     1561 agagatgttg cccaccggcg tgccccagcg cctcgtccac ggcaccgatg atcccaacgt
     1621 gccttacgag atcagcgagc ggtacgtacg ggccgcccag gagcgcggcg acaacgtccg
     1681 gctggtcaca ctgcccggcg cgggccactt cgaggtgata aacccggata cgaaggagtg
     1741 gcggcaggtc gtcagcacta tgctggaatt gatttagcgc gcgtctcaaa actctctggc
     1801 ctgagtgttc gtaatctcct gttccggcta gttgtggcga agggaacgct ctatgcaaaa
     1861 gatgtcgaag ccggtgagta tgttgtggct agccctggtg ataggcgacc tggccgtggt
     1921 tgcatacttg cattttgccg gtgggttggc atacacgccg gtccctgact tgccgttact
     1981 ctactgggtg ctgctgctcc tggtgcctgt gctgcaactc gctcccgtgg tggttcttgg
     2041 cgtcgtgcct atgccgagag ggcgctcaag tgaagcaagt cttggggtac tgctcctttc
     2101 gggcgggttg tctctccttc tcgcgttgtt caaccatctt acacagcccg agtggggcgg
     2161 caatacagtg ctatttgctg cgttgggctg tatatctttc gcctccgcgt atactatgta
     2221 caggcggatg caggcttcga aataggcgca gatagccagc aagatgtaac aaccacgcca
     2281 ctgcgatcta accgcccaac gtcgaacaga ggaagccccc aatgcccgaa gaaacctggc
     2341 aagtgctcgg cagcgagcct gtgatagagc acccgttcct gaaggtaagc gtgcagcagg
     2401 tacgcttgcc ggacggacgt gtgattcccg actggcacat cgtgcacacg cgggactacg
     2461 tgaacgtgtt cgtgctgaac gaggccgggc aggcgctcat ccttgagggg tacaaacacg
     2521 ggctggggaa gtcgagctgg caggtggtcg gcggctatgt ggaggacgag gaggaccccc
     2581 tgcgcaccgc gcaacgcgaa ctgctggaag aaacaggcta tgcgagcgac gattggcggc
     2641 acctggggag cttcgtggtg gacgccaacc gtcacgtcgg caagggccac ttcttcctgg
     2701 ctcgtgatgc ccgccttgtc gcccggcctg accacgacga cctggaaggc ttcgaggtcc
     2761 ggtgggtgtc gcttgaggaa gtgggcgcgg ccctgcggga cggcagggtg gggattatca
     2821 gctacggggt gaatatcgca ttggcgctgc tggccctgcg atagccctgt gggggtcccc
     2881 taagcccccg cgtcggcccc ttccatctcc gcatccaccg ccacatgctg gcctcgatgc
     2941 tcgttgttct cctcggcggc gcgctgggtg ggggccatga tgggcgagcg ggtgaagccg
     3001 gactggctct cccagccgct acgggcttcg tcgcccggct ccccctgcgg gccgggcagc
     3061 aggcgcacgg cgagttgcag actcctggcg aacaggccgg ggaagagggt gctggcgagt
     3121 atccccaggc gggcctgtat cgtggtcgta agctccgggg agccgttgcg gcaggcctca
     3181 attatctggc gggcggccct ctgcgcgctc atcgcagtca gcgggaccgt gcccagcaac
     3241 atgaacaggc cgaactcctt cttcctatcg cccttgaaga gcgcgttata gggcgagccg
     3301 gtgcgcatca ggccgggggc caccgtggtg acgtggatgt tgtccttggc aagctcggcg
     3361 cgcatgccgt ccgacaggcc gactagggcg aacttgctcg cgacgtaggg cagcaggtga
     3421 ggcacggcta tcttgccgcc tatcgaggcg atgttgacga tgcggccttc cccctcgcgc
     3481 ttcatgtgcg ggattaccgc ctgcatagcg tacagcggcg cccacaggtg caccttcata
     3541 gcgtcctcga agtcctgcaa ctgcatgtgc tccacggggc cgacctggat gacgcccgcg
     3601 ttgttgacga gcacgtctat ccgcccgcga tgctctatca cctgccgcag ggtgttctcc
     3661 agttgctggc ggtcggtcac gtcgcagcgg aaggcgagca cgtcggcccc accgacagag
     3721 atgtcctgta cggcgcggtc gagctgctcc tgggtgcggg ccaggatggt cactcgcgcc
     3781 ccctcgtcgg ccagcaggcg ggccatgatc agccccagcc ctcgcgagcc gccggtgatg
     3841 accaccgact tgtccatgaa cgtatacggt ttgctcttct tagttacgtt ggccgcgacc
     3901 gctacgcccg ccccagccgc cagggccgtc gccagtatgg tgcctttcat tgtggtgtct
     3961 ccatttcggt gagctttatg ccatagttat gagatccatg agcaggaatc gtgctagccg
     4021 gcacacagta agggtatgat gcatcgtacc caggagggta gcccttgcgc atggaagaac
     4081 catattcccc ctgtcatcct gagcgcagcg aaggatcagg tgctgtagt
//
LOCUS       DASRWZ010000455         5472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_723083, whole genome shotgun sequence.
ACCESSION   DASRWZ010000455 DASRWZ010000000
VERSION     DASRWZ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723083"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VFH60_07095"
     CDS             complement(<1..720)
                     /locus_tag="VFH60_07095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HET6313585.1"
                     /translation="MHRFYVPHARPRDGKLQLTGPVYEQIRRVLRMRPGDAVAIFDGT
                     GREYQLRLLEFGKGEVYGQITDQYPVATEPRCQVEIYLSLLNKSDKFEWALQKCTELG
                     ATHFVPVVAARSVTASGRHERWERIIQEAVEQSGRGVLPLLSGVQPFQQAIRQAVEQP
                     LTLALMPEVTGDRSLGEALANRQPNLDRVALFIGPEGGFSPEEQAFAIEQGVNVVKLG
                     PRVLRAETAAVAALTVTMERLG"
     gene            complement(748..2016)
                     /locus_tag="VFH60_07100"
     CDS             complement(748..2016)
                     /locus_tag="VFH60_07100"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HET6313586.1"
                     /translation="MDPYLPTMILLFGVTLALSLGLTPVVRWAALRLGWVDRPGEARR
                     VHKVPVPRLGGLAIYAAFAVGLLLTFIFPLRGPYAPGDNARFEGGRVLLMLAGAGLIA
                     LVMAIDDVKGLKPLPRLFWQLGVASLMVVPSLLVPGGANPTDADPSTRHYDQGAGILA
                     TGIQNPFRGMTIPFLGPEPLGQTIELWLPLAVVFTVVWIAGVTNTINWIDGLDGLAAG
                     VTVVACFVLFVLTVSVQQYTLAYVPLLLLAAVLGFMPYNIHPARIFMGDSGAMFLGFT
                     LAVISIMGGAKLAAALLVLGIPLLDSFYMIIYRLMRGRSPISADRGHLHHRLHDMGLS
                     HGQVVIIFMVLCGAFGLVGMINSSNTGGGADNGNAIWSIVKLGTMVVMALVLAGIAVY
                     ISRRRFDRNDTPRDQGSPSSRGVQTSTPDS"
     gene            complement(2103..2732)
                     /gene="upp"
                     /locus_tag="VFH60_07105"
     CDS             complement(2103..2732)
                     /gene="upp"
                     /locus_tag="VFH60_07105"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET6313587.1"
                     /translation="MNNIVQVSEHPLIQHKLALMRSVKTEPKKFREVVREITSLLLYE
                     ATLDLETVQTDVQTPLATMTGCKLADRIGFVPILRAGIGMVDAALELIPTAQVWHIGL
                     YRDHRTLLPVEYYNKLPIQPTVDLCIVLDPMLATAGSATATVKILKDWGARKIKFVGL
                     IAAPEGIERLHGEHPDVPIHVAAVDTHLNDVGYIVPGLGDAGDRQFGTS"
     gene            complement(3053..4351)
                     /gene="glyA"
                     /locus_tag="VFH60_07110"
     CDS             complement(3053..4351)
                     /gene="glyA"
                     /locus_tag="VFH60_07110"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET6313588.1"
                     /translation="MYTTLPLDPQEIGAPRPVDRPLRDTDPDLWQAIEDEKRRQRDKI
                     ELIASENYVSRAVLEAQGSVLTNKYAEGYPGRRYYGGCEYVDVAETLAIARAKEIFGA
                     DHANVQPHSGAQANMATYMAVLKPGDKILGMGLAFGGHLTHGYSINFSGALYQASSYP
                     VDRETERIDYDALEQQAMEERPRMLVVGASAYPRVIDFERCRQIADACGAMLMVDMAH
                     IAGLVAAGIHPSPVPHADFVTTTTHKTLRGPRGGMVLCKAEHAKAIDKSVFPGIQGGP
                     LMHVIAAKAVAFKEALEPGFHDYQRQIVANAQRLAEGLEGAGLRLVSGGTDNHLMLVD
                     VSPLGLTGKVAESALDEVGITVNKNAIPYDPQPPAKASGIRVGTPAVTTRGFREPEMD
                     TIARLIGEVLRAPEDPATKERVSAEVLELCSHFPVPGIEA"
     gene            4998..>5472
                     /locus_tag="VFH60_07115"
     CDS             4998..>5472
                     /locus_tag="VFH60_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6313589.1"
                     /translation="MSNTSAENVGGAQEHGQLSVLVTGGAGYIGSVAVEMLADAGYRV
                     VVFDNLIKGHQGAIDPRATFVDGDIADLPLLERTLKEHQVDAVMHFAAHSLVGESMEN
                     AVKYFTNNVTASVTLVEAMIRAGTKMLVFSSSAATYGMPRSSPIQETDPTAPINPY"
BASE COUNT          943 a         1474 c         1981 g         1074 t
ORIGIN      
        1 gccaagacgc tccattgtga cggtaagggc ggctacggct gcggtctcgg ccctgaggac
       61 gcgcgggcct agtttcacca cgttgacgcc ctgctctatc gcgaaggctt gctcctcggg
      121 ggagaagccg ccttcggggc cgatgaagag ggctacgcgg tcgaggttgg gctggcggtt
      181 ggcgagggct tcaccgaggg agcggtcgcc ggtgacttcg ggcatgaggg cgagggtgag
      241 gggttgctcg acggcctggc ggatggcttg ctggaagggc tggacgccgg agagtagggg
      301 cagcacgcct ctgccgctct gctcgactgc ctcctggatg atgcgctccc aacgttcgtg
      361 gcggcccgag gcggtaacgc tgcgagcggc gactaccggc acgaagtggg tggcccccag
      421 ctcggtgcat ttttgcaagg cccactcgaa cttgtccgac ttgttgagca gcgacaggta
      481 aatctccacc tggcagcgcg gctcggtggc aaccgggtac tggtcggtga tttggccgta
      541 gacctcgcct ttgccgaact ccaggagcct cagttggtac tcgcggccgg tgccgtcgaa
      601 aatggctacc gcgtcgccgg ggcgcatgcg gaggacgcgg cgtatctgct cgtagacggg
      661 gccggtgagt tgcagtttgc cgtctcgcgg cctggcgtgt gggacgtaaa agcggtgcat
      721 atggtaagtg cgctccgcgt gagagtgtta gctgtcgggg gtggaggtct ggacgccacg
      781 ggaggagggg ctaccctggt cgcgtggggt gtcgttcctg tcgaagcgtc ggcgggatat
      841 gtatacggcg atgcccgcca gcaccagggc catgaccacc atcgtgccga gtttgactat
      901 ggaccagatg gcgttgccgt tgtccgcgcc gccgccggtg ttgctgctgt tgatcatacc
      961 gaccagcccg aacgcaccgc acagcaccat gaagatgatt accacctggc cgtggctgag
     1021 gcccatgtcg tgcaggcggt ggtgcaggtg tccgcggtcg gcggagatgg gagagcggcc
     1081 gcgcatgagg cggtagatga tcatgtagaa gctgtcgagc aggggtatgc cgagcacgag
     1141 cagagcggct gccagcttgg ccccgcccat gatggagatg acggccaggg tgaagccgag
     1201 gaacatcgcg ccggagtcgc ccatgaagat gcgggcgggg tggatgttgt agggcatgaa
     1261 gccgagcacg gcggcgagga ggagcagggg cacgtaggcc agtgtgtact gctggacgct
     1321 caccgtgagc acgaacagga cgaagcacgc caccaccgtg acgcccgccg ccaggccgtc
     1381 gaggccgtct atccagttga tggtgttggt cacgcccgcg atccagacga cggtgaagac
     1441 cacggcgagg ggcagccaaa gctcgattgt ctggccgagg ggttcgggcc cgaggaaggg
     1501 gatggtcata ccccggaagg ggttctgtat gccggtggcg agtatgcccg cgccctggtc
     1561 gtaatggcgg gtgctggggt ctgcgtcggt ggggttcgcg ccgccgggca cgagcaggct
     1621 gggcacgacc atgagggagg cgacgcctag ctgccagaag aggcggggca ggggcttgag
     1681 gccctttacg tcgtctatag ccatgacgag ggcgatgagg ccggcacctg cgagcatgag
     1741 gagcacgcgg ccaccctcga agcgggcgtt gtcgccggga gcgtaggggc cgcgcagggg
     1801 aaagatgaag gtgagcaaca ggcccaccgc gaaagccgca tagatggcaa gcccacccag
     1861 gcggggcacg ggtaccttgt gcacgcgccg ggcctcgcct ggcctatcca cccaacccag
     1921 gcgcaacgcc gcccaccgca cgaccggggt aagccccagg gacagcgcga gggtgacgcc
     1981 gaacagcaat atcattgtgg gcagataagg gtccaagggt tctccatttt agatttcgga
     2041 ttttggattt tggattgctt ggggttcttc ttaatccaaa atccaaaatc cgaaatctaa
     2101 aatcacgagg ttccgaactg gcggtctccg gcgtcgccga ggccggggac tatgtagcct
     2161 acgtcgttga ggtgggtgtc tacggcggct acgtggatgg gcacgtcggg gtgctcgccg
     2221 tggaggcgct cgatgccttc gggggcggcg atgaggccga cgaacttgat tttgcgcgcg
     2281 ccccagtcct tcaggatttt gaccgtggcg gtggcggagc ctgcggtggc gagcatgggg
     2341 tctagcacga tgcagaggtc cacggtgggc tggatgggga gcttgttgta gtattcgact
     2401 ggaaggaggg tacggtggtc gcggtagagg ccgatgtgcc agacctgggc ggtaggtatc
     2461 agctcaagcg cggcatcgac catgccgatg cccgcgcgga ggatgggcac gaagcctatg
     2521 cggtcagcca gcttgcaacc tgtcatcgtg gcgagagggg tctgcacgtc ggtttggacc
     2581 gtttcgaggt ccagggtagc ttcgtatagc agcaggctgg tgatctcgcg cactacctcg
     2641 cggaacttct ttggctcggt tttgacgctc cgcatgaggg ccagcttgtg ctgtattagg
     2701 gggtgttcgg agacttggac tatgttgttc atgtggtttt ctctgatgtt ggttagtgac
     2761 gaatgctaga ggaacagcct ctcgcggatg tcacattgtt ggcctcgctg ggccaaacct
     2821 ctccttgtca tcctgagcgc agcgaaggat cagtaacccg ttcttgaccg ttactattga
     2881 tgtacccact tccccatgcc gccagcatcc cgcacgttga agactctcgc ctacccacct
     2941 gatccttcgc tacgctcagg atgacaaaga gggagttcgg ctgcgcgggg ccaacaattt
     3001 cgcgcacgcg agagtctgcc cgctgtcctc agcattcgtc acttatcact cgtcacgctt
     3061 cgatgccagg gacggggaag tggctgcaaa gctccagcac ttcggcggac acgcgctctt
     3121 ttgtggcggg gtcttcgggg gcgcggagca cttcaccgat gaggcgggct atggtgtcca
     3181 tctctggctc gcggaagccc ctggtggtga cggcgggcgt gccgacgcgg atgccggagg
     3241 ctttggcggg gggctgcggg tcgtagggga tggcgttctt gttgacggta atgcctacct
     3301 cgtccagggc gctttcggct accttgccgg tgaggccgag gggggatacg tccacgagca
     3361 tgaggtggtt gtcggtgccg ccggagacga ggcgcaggcc cgcgccctca agcccttcgg
     3421 ccaggcgctg agcgttggcg acgatctggc gctggtagtc gtggaagccg ggttcgaggg
     3481 cctctttgaa ggctacggct ttggccgcga ttacgtgcat gagggggccg ccctggatgc
     3541 cggggaagac gctcttgtct atggccttgg cgtgctccgc cttgcacagg accatgccgc
     3601 ctcgggggcc gcgcagggtc ttgtgggtgg tggtggtgac gaaatcggcg tggggcacgg
     3661 gggaggggtg gatcccggcg gcgacgaggc cggctatgtg ggccatgtcc accatgagca
     3721 tcgcgccgca ggcatcggca atctggcggc agcgctcaaa gtcgattacg cgggggtagg
     3781 cgctggcacc gaccacgagc atgcggggcc gctcctccat agcctgctgt tcgagggcgt
     3841 cgtagtctat gcgctcggtc tcgcggtcca cgggatagga agaagcctgg taaagggcgc
     3901 cggagaagtt gatggagtag ccgtgggtga gatggccgcc gaaggcgagg cccatgccga
     3961 ggatcttgtc gcccggcttg agcacggcca tgtaggtagc catgttggcc tgagcgccgg
     4021 agtggggctg cacgttagcg tggtcggccc cgaaaatctc tttggcgcgg gcgatggcga
     4081 gggtctcggc tacgtccacg tactcgcagc cgccgtagta acgcctgccc gggtagccct
     4141 cggcgtactt gttggtgagc acggagcctt gtgcttcaag gacggcgcgg ctgacgtagt
     4201 tctcggaggc gataagctct atcttgtcac gttggcgacg cttttcgtcc tcgatggcct
     4261 gccagaggtc gggatcggtg tcgcggaggg ggcggtccac ggggcggggt gcgcctattt
     4321 cttgcgggtc gaggggaagc gtggtataca ttggcgttac tccatctggt gagatgtacg
     4381 gagtgttggg ttttgagttg ggcgtgagga ttgtgcaagt gtacggggtg gtgcctggat
     4441 gctttgcggg gccattatac aactttgggt ggctgggggc taatgtatgc acgggagcgg
     4501 gtgtaggggt atggcttgtc gtagcctggt gggcagtgct cttgcgggac cagagcgaca
     4561 atatcccgcc ttgtcatcct gagcgtagcg aaggatcagt agcccgttct tggccggttc
     4621 aattgttgct ctaatgttcc acttaccgcc agcatcctga gcgttgaaga ctctgtccta
     4681 cccacctgat ccttcgctac gctcaggatg acaagggggg agtatggtcc tgtagagcat
     4741 tgcatcgggg ctgctgacca ggggagggtg aggacatctt ctacgtcggt ccttcaccgg
     4801 actaaatgtg gaggccgcac gagagggcat tagggcaacg gggtactagt tagttgctac
     4861 tccttacgcg tggggtactc acaccggggt atgcttcggg tagtaaatgg ggcagtttac
     4921 ccctaaagtg ttagccgaaa aataacaccg acaatagccc gtttgggaca tcaattgagc
     4981 atgggaggtc tgctataatg agcaacacat cggctgagaa cgtgggtggc gcgcaggagc
     5041 acggccagtt gagcgtgctg gttaccggcg gcgcggggta catcggcagc gtggctgttg
     5101 agatgctggc ggacgccggg tacagggtgg tggtgttcga caacctcatc aaggggcacc
     5161 agggggcaat cgaccctcgc gctaccttcg ttgacgggga tatcgcggac ctgccgttgc
     5221 tggaacggac gctcaaggag catcaggttg acgcggtgat gcacttcgcg gcccactcgc
     5281 tcgtgggcga gtcgatggag aacgcggtca agtactttac taacaacgtc acggcgagcg
     5341 tcacgctggt cgaggccatg atacgcgccg ggaccaagat gctggtcttc tcgtcaagcg
     5401 cagctacgta tggcatgccg cggagcagtc cgatccagga aacggacccc acggctccca
     5461 tcaatcccta tg
//
LOCUS       DASRWZ010000456         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_654530, whole genome shotgun sequence.
ACCESSION   DASRWZ010000456 DASRWZ010000000
VERSION     DASRWZ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654530"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..873
                     /gene="hslV"
                     /locus_tag="VFH60_07120"
     CDS             301..873
                     /gene="hslV"
                     /locus_tag="VFH60_07120"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET6313590.1"
                     /translation="MILSTTILAVRRDNTVAMAGDGQVTIGDVVMKHSARKIRRLYKD
                     RVLAGFAGAVADALTLFDKFEAQLERNKGNLRKSAVELAKEWRTDRYLRRLEAQLIVA
                     DPSQLLILSGEGEVIEPDDGAVAIGSGGSYALAAARALLAHTDMPADLIAQAALRIAG
                     DLDIYTNSNIAFYSLSQEVPGDGAKGQDNL"
     gene            923..2452
                     /gene="hslU"
                     /locus_tag="VFH60_07125"
     CDS             923..2452
                     /gene="hslU"
                     /locus_tag="VFH60_07125"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET6313591.1"
                     /translation="MQDLTPRQIVVELDKYIVGQGAAKRAIAVALRNRVRRQKVDPTM
                     RDEITPKNLMLIGPTGVGKTEIARRVARLIDAPFIKVEATKFTEVGYVGRDVESIVRD
                     LAEAAFSLEHDQRIASVRDQAESVATERLINMLMEQRDDLRAKPARKGRQRVQAQAQA
                     QVGGPDVPMPATLDGGGGSSLAPTITASIDSEVRVLSEVSERALKTQRRKVARLLAEH
                     KLDEEQIEVDVNDLGLDDDLGGVLEFSPGMSAEEMSESFSDFLDGYRSMQSSLNGLSR
                     KRVRRMSVREAKRFLVEEEAHKLVDMDAIVESAMRRAEQNGVVFIDEIYKITGSAIDT
                     GPDVSGEGVQRDMLPIVEGSTVQTRYGPLKTDHVLFIAAGAFHGSKPSDLIPELQGRF
                     PLRVELESLGEEQLQAILTEPENALTRQYTALMKTEGVDLAFSEDGAARIAQVAAELN
                     GRVEDIGARRLHTIMEQVLEDLSFRADEEQGNTITIDASYVDGRVKQIAKDEDLSRFI
                     L"
BASE COUNT          546 a          812 c          841 g          435 t
ORIGIN      
        1 tccgagccct tcggctatgc tcagggtaaa ctccgcgagg aatcaggcgg gtagggaaga
       61 aggtgcattg cagtaattga gtggccctgc taccctcggc ccccgttgca acagtgttcg
      121 actgccacct gattcctcgc ggagtttacc ctgagcatag ccgaagggct cggaatgaca
      181 agggggaggt ccgtgctgct cgccggggta gcaggggctt ttgcacactc cgaaaacatt
      241 tccacatttg cgcggcgttt ctccatcccc gtataattga cgcagaggca cactgacagc
      301 atgatactta gcacaactat tttggcggtg cggcgcgaca ataccgtggc aatggccggg
      361 gacggccagg ttaccatcgg ggatgtcgtg atgaagcatt cggcccgcaa aattcgtagg
      421 ttatacaagg accgggtgct cgccgggttc gccggggcgg tggccgacgc gctgaccctc
      481 tttgacaagt ttgaagccca gctagaacgc aacaaaggca acctccgcaa gtccgccgtg
      541 gaactggcga aggagtggcg caccgaccgc tacctgcgcc gcctggaggc ccagcttatc
      601 gtggcggacc cctcgcaact acttatcctt tcaggtgaag gcgaagtaat cgaaccggac
      661 gacggcgcgg tagccatcgg ctcaggcggc tcttacgcgc tggcggcagc acgcgccctg
      721 ctcgcccata ccgacatgcc tgcggacctc attgcccagg ccgcgttgcg tatcgcgggc
      781 gacctcgata tatataccaa cagtaacata gctttctact ctttatctca agaagtaccg
      841 ggcgacgggg cgaaggggca ggacaactta tagcccggct gcccgtgaca tggaccggaa
      901 actcgctaga gaggcaattc acatgcaaga cctgacacct cgccagatag tggttgagtt
      961 agacaaatac atagtggggc agggcgcggc caagcgagcc atagccgtgg ccctgcgcaa
     1021 tagggtgcgc cgccagaagg tggaccccac tatgcgcgac gagattacgc ccaagaacct
     1081 gatgctgatt ggccccaccg gcgtcggcaa gacggagatc gcccgccggg tggcccgcct
     1141 gatagacgcc cccttcatca aggtggaggc gaccaagttc actgaggtcg gatacgtggg
     1201 gcgcgacgtg gagtccatcg tgcgcgacct ggccgaggcc gccttcagcc tggagcacga
     1261 ccagcggata gcctccgtgc gcgaccaggc agagtcggtg gctaccgagc gccttatcaa
     1321 catgctgatg gagcagcggg acgacctgcg cgccaagccc gcaaggaagg gccgccagcg
     1381 cgtgcaggcc caggcccagg cgcaggtggg cggccccgac gtgcccatgc ccgccacgct
     1441 ggacgggggt ggggggagca gcctagcacc cacgattacc gccagtatag atagcgaggt
     1501 gcgcgtgctg tccgaggtgt ccgagcgcgc gctgaagacg cagcgccgca aggtggcccg
     1561 cctgctcgcc gagcacaagc tggacgagga gcagatcgag gtggacgtga acgacctcgg
     1621 cctggatgac gacctcggcg gggtgctaga gttctcgccc ggcatgtcgg cggaggagat
     1681 gtccgagtcg ttctccgact tcctggatgg ctaccgctcc atgcaatcga gcctgaacgg
     1741 cctcagccgc aagcgcgtgc gccgcatgtc ggtgcgcgag gccaagcggt tcctggtgga
     1801 ggaggaagcc cacaagctgg tcgatatgga cgctatcgtg gagagcgcca tgaggagggc
     1861 cgagcagaac ggtgtagtgt tcatagacga gatctacaag atcaccggct ccgctataga
     1921 taccggcccc gacgtgtcgg gcgagggcgt gcagcgcgac atgctgccca tcgtggaagg
     1981 ctccaccgtg cagacccgct acggccccct caagacggac cacgtgctgt tcatcgctgc
     2041 gggcgcgttc cacggctcca agccctccga cctgataccg gagcttcagg gccgcttccc
     2101 gttgcgcgtg gagcttgaga gcctgggcga ggagcagcta caggcgatac tcactgagcc
     2161 tgagaacgcc ctcacccgcc agtacaccgc cctgatgaag acagaggggg tagacctggc
     2221 gttcagcgag gacggcgccg cccgcatcgc ccaggtagcc gccgagctca acggccgcgt
     2281 ggaggacatc ggcgcccgcc gcctgcacac gatcatggag caggtgctgg aagacctctc
     2341 cttccgcgcc gacgaggagc agggcaacac catcaccatc gatgcgtcat acgtggacgg
     2401 ccgcgtgaag cagatagcca aagacgagga cctgagccgc ttcatcttgt agccgcccgg
     2461 cacctcggta cgtgatatta ggacgagatg aaaccgcaca ggcgcaaaga gcgcaaagga
     2521 cacggaacga taaagtgggt gttctaagca gaaggaccag cgtacatcta acgtaccctg
     2581 gtccttcttc tacccagtaa accttcactt tcttcccgtg tcctttgcgc tctt
//
LOCUS       DASRWZ010000457         1754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_106530, whole genome shotgun sequence.
ACCESSION   DASRWZ010000457 DASRWZ010000000
VERSION     DASRWZ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106530"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFH60_07130"
     CDS             complement(<1..675)
                     /locus_tag="VFH60_07130"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HET6313592.1"
                     /translation="MQEKDIVPQSWTELLDRLYEESWNERLGRFRSNFAYRGMQDSRF
                     DLTTTLVRLGGDYPRHEEDLLRNFRKYAHKQAAMGDSLWYWLSLAQHHGLPTRLLDWT
                     YSPFVALHFATAEIELFDTDGLIWCVDYIKTRQLLPRKLRDILEQEDTNIFTAEMLSQ
                     AARSLREFDDLSKDDYVVFIEPPSLDERSVNQYALFSLMSSPSANLEAWLREHPECHR
                     RVIIPAS"
     gene            complement(719..1576)
                     /locus_tag="VFH60_07135"
     CDS             complement(719..1576)
                     /locus_tag="VFH60_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313593.1"
                     /translation="MGLPAVKELVDSLGIERPHLFELLFLRVVTGSYGDVPVSPAQVR
                     AWDPYSTQDTHAEAFAALAAKGLVTEDEQDNVGLSVAGSEVVDRLHEAGRAFVAAHDP
                     LPPGELETVSALLGAAVEATLADPALAPRPGSHLAGSRSLATFDPDAPAMVRIEQAIY
                     DLWHARDDAHMMTWRDAGLEGPAMPVFTLVWSGEAKTLPELAERMGGSGAVAQIESSL
                     AYLVASEYLEVDGEQVRLTPAGALVRDDIEHETDRIYFAPWPLSLEDAEWLRDHLRQL
                     VAHLPVPPL"
BASE COUNT          316 a          572 c          560 g          306 t
ORIGIN      
        1 cgaggccggg ataatcacgc ggcggtggca ctcagggtgc tcgcgcagcc acgcctcaag
       61 gttggcgctg gggctcgaca tcagcgagaa cagcgcgtac tggttcacac tccgctcgtc
      121 cagcgacggc ggctctataa acaccacgta gtcgtccttg gagaggtcat cgaactcgcg
      181 cagggagcgg gcggcctggc tcaacatctc ggcggtgaag atgttggtgt cctcctgctc
      241 caagatatcg cgcagcttgc ggggcagtag ctggcgcgtc ttgatgtagt ccacgcacca
      301 tatcaggccg tcggtgtcga acaactctat ctcggcggtg gcgaaatgga gggccacgaa
      361 gggcgaatag gtccagtcga gcaggcgggt gggcaggccg tggtgctggg ccagcgacag
      421 ccagtaccag agcgagtcgc ccatagccgc ctgcttgtgc gcgtacttgc ggaagttgcg
      481 cagcaggtcc tcctcgtgcc gggggtagtc gccgcccagc ctcaccagcg tcgtggtgag
      541 gtcgaaccgc gagtcctgca tgcccctgta ggcaaagttc gacctgaacc gccccagccg
      601 ctcgttccag gactcctcgt agaggcggtc cagcagttcg gtccagcttt ggggtactat
      661 gtctttctct tgcacggcag gctcgctttg gaagtagtac gatggaagat ggacgatgtt
      721 acagcggagg cacgggcagg tgggctacta attggcgcag gtgatcgcgc agccactcgg
      781 cgtcttccag ggataggggc cagggggcga agtagatgcg gtcggtctca tgctctatgt
      841 cgtcgcgcac cagggcaccg gcgggcgtga gtcgcacctg ctcgccatct acttccaggt
      901 actcactggc aaccaggtag gccaggctgc tctcgatctg ggcaaccgcc cccgaaccgc
      961 ccatgcgttc ggcaagctcg ggcagcgtct tcgcctcacc cgaccagacg agcgtgaaca
     1021 ccggcatagc cggaccttcc agcccggcat cgcgccaggt catcatgtga gcgtcgtcgc
     1081 gggcgtgcca cagatcgtat atcgcctgct cgatgcgcac catcgcggga gcgtcggggt
     1141 cgaacgtcgc cagggagcgg ctgcccgcca ggtgactgcc aggccggggc gcgagcgcgg
     1201 ggtctgccaa ggtcgcctca acagccgcgc caagtagcgc actcaccgtc tccaactctc
     1261 cgggtggcaa tgggtcgtgg gcagccacga acgcccgccc ggcctcgtgc agccggtcca
     1321 ccacctcact gcccgccaca gaaaggccga cgttgtcctg ctcgtcctcc gttaccagtc
     1381 ccttcgcggc aagcgcggca aacgcctccg cgtgggtgtc ctgcgtggag taggggtccc
     1441 aggcgcgcac ctgcgcaggc gaaacaggca catcaccgta gctgcccgtg accacccgca
     1501 gaaacagcag ttcgaacagg tgcggtcgct cgatgcccag gctgtccacc aactccttga
     1561 cggcaggcag acccacacgg cgcttcctga gaaactgcgg catcaagggc ggaagttccc
     1621 tcaacgcggc cgtccgtgct tcaccaacgc ttgcattcat gggcctaatc ttagcacctc
     1681 catgcccgat tgaccagagg gcaaattcca actgtgcgcc tgtccgtgac tgattttttc
     1741 accgcagaga cgca
//
LOCUS       DASRWZ010000458         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1271778, whole genome shotgun sequence.
ACCESSION   DASRWZ010000458 DASRWZ010000000
VERSION     DASRWZ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271778"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..788)
                     /locus_tag="VFH60_07140"
     CDS             complement(294..788)
                     /locus_tag="VFH60_07140"
                     /inference="COORDINATES: protein motif:HMM:NF026248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HET6313594.1"
                     /translation="MPGGLELQWAGPVFAVVTFLTIWWGHILVRIVHYHLGTRPAPVI
                     FLGGLLLLLGSTQTGNDILGGTCGIVGLTLLWDAFELFRQEARVRQGHAPLNPRVHRA
                     AAGLAAARFAEEHPEADEGGTCLSCGSTPNSRYLCRRGPYCQVRHWERYATPQTAEVE
                     AREG"
     gene            complement(790..1149)
                     /locus_tag="VFH60_07145"
     CDS             complement(790..1149)
                     /locus_tag="VFH60_07145"
                     /inference="COORDINATES: protein motif:HMM:NF026248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HET6313595.1"
                     /translation="MHWTGIGLGLFSALVIGLGFVWVIRFEYYVGAYRWKWVAAAGLL
                     ICAASLIMPNFLLSAITGIVGGSIVWGAMELPHQEERVEHGLFRAHPNRASRPSLHHD
                     GGHDHGSKPGLGKWWSF"
     gene            complement(1218..1388)
                     /locus_tag="VFH60_07150"
     CDS             complement(1218..1388)
                     /locus_tag="VFH60_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313596.1"
                     /translation="MTLDDIGGWPLAVLAVLALAAATVAFALLIGRWIDAQSNSVVDP
                     PRKGRKRDSGSE"
BASE COUNT          303 a          510 c          565 g          300 t
ORIGIN      
        1 ggtgccgcca gcgacggcgc gtccaggaag gtaattgagg gcacagccac ggcaatccag
       61 cccaaggccg actagcgggg aggcggaaac gccaagacgc caagaaacac gccaagatta
      121 aggattgttg caccagcaaa aggaccaggg tatgccacat gtaccctggt cctttttgta
      181 ctctccaaac ctcaactaac ttggcgtggt tcttggcgtc ttggcgtctt ggcgtttccg
      241 cctgtagatg gaactattat cgtggtactg caattttcgg gctgctcagg tggttagcct
      301 tcgcgggcct cgacttccgc ggtttggggt gtggcgtagc gctcccagtg gcggacctgg
      361 cagtaggggc cgcgcctgca caggtagcgg ctattgggtg tggagccgca ggagaggcag
      421 gtgcccccct cgtcggcttc cgggtgctcc tctgcgaagc gggcggcggc gaggccggcg
      481 gctgcgcggt gcacgcgagg gtttagcggg gcgtggccct ggcgcacgcg ggcctcctgg
      541 cggaaaagct cgaaggcgtc ccagaggagg gtgaggccga caatgccgca ggtccccccc
      601 agtatgtcat tgccggtctg cgtggacccc aggagtagca gcagccctcc gaggaatatt
      661 acgggagcgg ggcgcgtgcc caggtggtag tggacgatgc ggacgaggat gtggccccac
      721 cagatcgtca ggaacgtgac gacggcgaat accggtccgg cccactgcaa ctctagtccg
      781 ccgggcatgt tagaaactcc accacttgcc gaggcccggc ttcgagccgt ggtcgtggcc
      841 gccgtcgtgg tgcaggctgg ggcgagaggc gcggttgggg tgcgccctga agaggccgtg
      901 ctccacccgc tcttcctggt ggggcagctc catcgcgccc cacacgatgc tgccgcccac
      961 gatgccggtg attgccgata gcaggaagtt gggcataatg agggaggcgg cgcatatcag
     1021 cagcccggcc gccgccaccc acttccacct gtatgcgccc acgtaatact cgaagcggat
     1081 gacccatacg aagcccaggc caatcaccag ggctgagaat agtccgaggc cgatgccggt
     1141 ccagtgcaaa agcgctacct cctggggagc aaaatcgtaa cggtggggtc cagggaatta
     1201 tacaacaggc gggccatcta ctcactgccg gagtcgcgct tgcggccctt cctgggggga
     1261 tcgaccacgc tgttgctctg cgcgtcaatc cagcgtccta tcagaagagc aaaggccacc
     1321 gtggccgccg ccagcgcgag cacggccagc acggcaaggg gccagccgcc gatgtcgtcc
     1381 agtgtcaagg taagtcctcc tggaagtagg gtggcgttgt tacttgtaac agagcggtgt
     1441 gcggccacgt tcgccctgtg ggtgtgccct gtgcccttag ccctgccaat gaattggcgg
     1501 gcttaccagg gacgaagtct cctacggaga ctatcttgca tgttcctcag tccccgcagg
     1561 ggactttgcc ccttctcagc ccgtggctat agccacaggg cgtcaccgca gggcgtcccc
     1621 ccagggcaat acgtgagctg agttggtctt ggcatctgcc gtagcgtctg gcggcacg
//
LOCUS       DASRWZ010000459         1925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1203185, whole genome shotgun sequence.
ACCESSION   DASRWZ010000459 DASRWZ010000000
VERSION     DASRWZ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203185"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFH60_07155"
     CDS             <1..217
                     /locus_tag="VFH60_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313597.1"
                     /translation="AYDCALYHISDSNVTTHKAVRDEWLKSHPGQAARKNDPLGLDLD
                     RIWARVAEIRADWPFGPQVPLWPTTPE"
     gene            416..1714
                     /locus_tag="VFH60_07160"
     CDS             416..1714
                     /locus_tag="VFH60_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET6313598.1"
                     /translation="MARIKLAYIGGGSTRAPGTMASFVNQGENFDGSEVVLIDLDPER
                     LGLVKTLAEKMARARGLDLTITTTTDRRGGLQDCDAVLTSFRPGGFEARYLDESIPLK
                     LGVIGQETQGPGGFFMALRSIHVMQGIVADMEAVCPGAILFNYTNPVNLVSEAVTHHT
                     DIPTISLCEGPIIFPRWLAKLAGLDPTKVDAAMIGLNHGSWSVRHLYDGEDMMPMVRD
                     AYERVQGDPSASKHDLRWLQLAATMGSIPADYFLYYYFKEEILAELREKPTTRSQDIM
                     AKVPDYWQHYREQAARDEPVLEPNRSRGGIHELELAIDVMDAIFNDRKEVWPVNVPNR
                     GAITDLPDDMVVEAQGYADRNGVTPLSLGELPRHVSGLVHMLGEYQALTAEAAWSGTR
                     KDAVRALASNPLCFSLRTAEALYDEMAAAHREYLPARLLH"
     gene            1731..>1925
                     /locus_tag="VFH60_07165"
     CDS             1731..>1925
                     /locus_tag="VFH60_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET6313599.1"
                     /translation="MRLVVTEDYASLSRTGAGMVAQLIAARPEANVVPATGDTPMGIY
                     AELAARRGRGELDASRLRVFQ"
BASE COUNT          354 a          636 c          633 g          302 t
ORIGIN      
        1 agcgtacgac tgcgcgctat accacatcag cgacagcaac gtgacgaccc acaaggcggt
       61 cagggacgag tggctgaagt cgcaccccgg acaggctgcc cggaagaacg atccgctcgg
      121 cctcgacctc gataggatct gggccagggt agcggagatt cgggccgact ggccgtttgg
      181 cccccaggtg cccctctggc ccactacacc cgagtagtcc cggcgtctga ggcgcggggc
      241 agcactaccc tttgcggttg ccggccgggc gacccaggcg cttcctgagg gtcatcctca
      301 caagtgcgtg cgcaatgact gcccaggcca ctgggcggcc agtctccttg tggctatttc
      361 tgtataatca ttgcagcaag ctttgcggca atgattatac acgcaaggag tggagatggc
      421 ccgcatcaaa ctggcataca taggcggggg tagcacgcgc gctcccggta cgatggcttc
      481 gttcgtcaac cagggggaga acttcgacgg ctcggaggtc gtgctcatcg acctcgaccc
      541 cgagcggcta ggcctcgtaa agacgctggc cgagaagatg gcccgcgcca ggggcctcga
      601 cctcacgata acgaccacca cggacaggcg cggcggcctg caagactgcg acgccgtgct
      661 gacgagcttc cggcccggcg gcttcgaggc gcgctacctg gacgagagca tcccgctcaa
      721 gctcggggtt atagggcagg aaacgcaggg tccgggcggc ttcttcatgg cgctgcgctc
      781 catccacgtc atgcagggga tcgtggccga catggaagcg gtatgccccg gcgctatcct
      841 gttcaactac accaacccgg tcaacctcgt gtcggaggcc gtgacccacc acacggacat
      901 ccccactatc tccctgtgcg aggggccgat catcttcccc aggtggctgg caaagcttgc
      961 gggcctcgac cccacgaagg tagacgccgc tatgatcggc ctcaaccacg gctcatggtc
     1021 ggtgcgccac ctctatgatg gcgaggatat gatgccgatg gtgcgtgacg cctacgagcg
     1081 cgtgcagggc gacccctcgg cgagcaagca cgacctgcgg tggctgcaac tcgcggccac
     1141 gatgggcagc ataccggccg actatttcct ctattactac ttcaaggagg agatactggc
     1201 cgagctgcgc gagaagccta ccacccgctc gcaggacatc atggcgaagg tgcccgatta
     1261 ctggcagcac taccgcgagc aggccgcgcg cgacgagccg gtgctggaac ccaaccgctc
     1321 gcggggcggc atccacgagc ttgagcttgc tattgacgtg atggacgcca tcttcaacga
     1381 ccgcaaggag gtatggcccg tgaacgtgcc caaccggggg gccatcacgg acctgcccga
     1441 cgacatggtg gtggaggcgc agggctacgc tgaccgcaac ggagtaacgc cgctcagcct
     1501 gggcgagcta ccccggcacg tttcggggct ggtccacatg ctgggcgagt accaggcgct
     1561 cacggcggag gctgcctgga gcggcacgcg caaggacgcc gtgcgggcgc tcgctagcaa
     1621 ccccctctgc ttctcgctgc gcaccgccga agccttgtac gacgaaatgg ccgccgcgca
     1681 ccgggagtac ctgcccgccc ggctgctgca ctaggccccg gcgccacacg atgcgattgg
     1741 tagtgaccga ggactatgcc tccctgagcc ggaccggggc cggtatggtg gcgcagctca
     1801 tagctgcccg gccggaggct aacgtagtgc ccgcgaccgg cgacaccccg atgggcatct
     1861 acgccgaact ggcggccagg cgagggcgcg gcgagttgga cgcctcgcgg ctgcgggtgt
     1921 ttcag
//
LOCUS       DASRWZ010000460         4951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1339784, whole genome shotgun sequence.
ACCESSION   DASRWZ010000460 DASRWZ010000000
VERSION     DASRWZ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339784"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1591
                     /locus_tag="VFH60_07170"
     CDS             320..1591
                     /locus_tag="VFH60_07170"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HET6313600.1"
                     /translation="MDETTADGQPPAHEVARPTTLIVGQSGGPTAAINSSLVGVLREA
                     RSQGIRRIYGMRYGIRGLLSGDLVDLSNLPTEVLPALQQTPSAALGSCRYHLTDADIP
                     LAMSTLQEHGVGHMVYIGGNDSADTSHRLATAAMDQGVDLKVIAIPKTIDNDLPVTDH
                     CPGYGSAARFIAIATLETTLDTRAMPDIYPVKILETMGRHAGWLAAASGLARATGWET
                     PNLIYIPECPKDVDEILQEVQAAYQARGQVVIVMSENLRARDGRPFAVAGMEALNGGQ
                     QGNVKPDFVDDFGHVYYRGTSAGTVLRQLITGRLDLRARLDQPGTLQRMSQAHMSEVD
                     LIEAEECGRAAVRGALAGTTDQMVILERAEGPTYRCTIGMADLEQIANVEKRLPADFH
                     TDEGNFVTDAFIDYALPLVGAPLPQFVELLA"
     gene            1652..2500
                     /locus_tag="VFH60_07175"
     CDS             1652..2500
                     /locus_tag="VFH60_07175"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HET6313601.1"
                     /translation="MEVHKVGVLGAGTMGNGIAQVFAASGYEVIMRDMGQLPLDRGIA
                     SIQKSLGKFVEKGKLSTDEREEALSRINTTTELRDLAGCDLIVEAIFENFEAKSAAFS
                     ELDGLLRPEALLASNTSSIDITRLAAVTKRPDRFIGMHFFNPVPLMSLVEIIRGLATS
                     DETYQTVRDLAVSLGKTPVEVKDYPGFVSNRILMPMINEAVFALFEGVASKEDIDTVM
                     KLGMNHPMGPLTLADFIGLDVCLDILYVLYDGFKDPKYRPCPLLVKMVQAGKLGRKTG
                     EGFYTY"
     gene            2664..3821
                     /locus_tag="VFH60_07180"
     CDS             2664..3821
                     /locus_tag="VFH60_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET6313602.1"
                     /translation="MDFALSEDQRLIKQTAREFADEQLAPLAAEYDRTEEFTAPQAKM
                     LAEMGFLGMMVPEEYGGSGLDTISYVLAMEEVSRACASSSVTMTVQNSLVCWPVTAFG
                     TDEQKRKYLVPLARGESLGCYALSEPAAGSDPASMATMAVEDGDSYIINGSKIFISNG
                     GVSKVALVWATTSKELKHRGITAFIVESDTPGFTVGAEEKKLGIRGSNTTELHFENMR
                     VSKANILGNVGDGFKIAMQTLDGGRIGIAAQALGIGQACIDASVRYAHQRQAFGKPLA
                     EIEAIQIKVADMATEVQASRLLTLHAAFMKDQGLRFSKEAAMAKLHASETATRVAHAA
                     VQIHGGYGFLRDFPVERLYRDARITEIYEGTSEIQRHVISNAVLREGRPTE"
     gene            3852..4763
                     /locus_tag="VFH60_07185"
     CDS             3852..4763
                     /locus_tag="VFH60_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6313603.1"
                     /translation="MSTVQFGLVVPGDALDTSRRHLYMEDVSRLLNYVKGHYHSAWFI
                     DHLQFGSTDVLEGWTALAYLSALHPELQWGHSVICQAFRNPALLAKMAATLQFMSGGR
                     YILGIGAGWHEEEHLAYGYDFPTGSARVEELDEALQIIKAMWTQESATFEGKHYRVKE
                     ACCEPKPQPIPTIMVGAFGPKMLRLAARHAGWWNVSSTPIDTYRGYLREFERACEEVG
                     RDVRTVRRSWGGGCVCAPTEREVARLASERHQPEEDFVGTPRQLIEQMQPFVELGVDY
                     FMLDCGGFPELTTVEMLVNEVLPALNR"
BASE COUNT         1006 a         1607 c         1503 g          835 t
ORIGIN      
        1 tacgacaggt gttgtcaagt aaagttccag cgcccggccg cgaaccacca cgtttgctcc
       61 ggggcggcag ggttgtgacc gggtcggccg cctgcatcgg tgctccgcat gacgccacgg
      121 ggcctatccc agggcaccgc acatcacact ccgagcccaa tgccctccgc cgagaacgac
      181 gcgcacggcg tgcctcacgc gcaaatcttg tcccttcccc tcccctatgg tatgatattg
      241 tgcctgcgcc cacagcacga gggctggaaa aagcggtggc ccaggactcc ccaatgcctt
      301 atatggagcg cggacgccaa tggacgagac caccgccgac gggcagcccc ctgctcacga
      361 agtcgctaga ccaactaccc ttatcgtcgg ccagtctggc gggccaaccg ccgctatcaa
      421 ttcgtcgctt gtgggcgtgc tccgagaagc gagatcgcag ggcatccggc gcatctacgg
      481 catgcgctac ggcatacgcg gcctcctatc cggggacctg gtggacctga gcaacctgcc
      541 caccgaggtg ctccccgccc tccagcagac cccttccgcc gccctcggat cctgccgcta
      601 ccacctcacc gacgctgaca tccccctcgc catgtccact ctgcaagagc acggcgtcgg
      661 ccacatggtc tacatcggcg gcaacgactc ggcggacacc tcccacaggc tcgccacggc
      721 cgctatggac cagggcgtgg acctcaaggt tatcgccatc cccaagacga ttgacaacga
      781 cctgcccgtc accgaccact gccccggcta cggctcggcc gcgcgcttca tcgccatcgc
      841 cacgttggag acgacgctcg acacgcgggc catgccggac atctacccgg tgaagatact
      901 cgaaacgatg gggcggcacg cgggctggct ggctgcagcc tccggcctgg caagggccac
      961 cggctgggag acccccaacc tcatttacat ccccgaatgc cccaaggacg tggacgagat
     1021 cctgcaagaa gtgcaggcgg cctaccaggc gcggggccag gtggtcatcg tcatgtcgga
     1081 gaacctgcgc gcccgcgacg gcaggccctt cgcggtggcg ggcatggaag ccttgaacgg
     1141 gggccagcag ggcaacgtca agcccgactt cgtggacgac ttcggccacg tttactacag
     1201 gggcaccagc gcgggcactg tgctgcgcca gcttattacc ggccgcctcg acctgcgcgc
     1261 ccgcctggac cagcccggca ccttgcagcg catgtcccag gcacacatga gcgaggtaga
     1321 cctcatagag gccgaggagt gcggcagggc ggcggtgcgc ggtgcgctcg cgggcaccac
     1381 cgaccagatg gtcattttgg agcgggcgga aggccccacg taccgctgca cgattggcat
     1441 ggccgacctc gaacagatag ccaacgtcga gaagcgcctg cccgccgact tccataccga
     1501 tgaaggcaac ttcgtcaccg acgccttcat cgactacgcc ttgcccctag tgggcgcgcc
     1561 ccttccgcag tttgtcgagc ttctggcgta gtcgccggac ggctcgttcg acgcaccgca
     1621 agagcacaca tttacaaccg gaggccccta agtggaagta cacaaagtag gtgtattggg
     1681 agcgggtacg atgggcaacg gcatcgccca ggtgttcgcc gcttccggct acgaggtaat
     1741 catgcgcgac atgggccagt tgcccctgga ccgtggcatc gcctcgatcc agaagagcct
     1801 gggcaagttc gtggagaaag gcaagctcag caccgacgaa cgggaggagg ccctctctcg
     1861 catcaacacg accacggagc tgcgcgacct ggccgggtgc gacctcatcg tagaggcgat
     1921 cttcgagaac ttcgaagcca agtcggcggc cttctcggag cttgacggcc tgctacgccc
     1981 cgaagccctg ctggcctcca acacctcctc catcgacatc acccgcctcg ccgccgtaac
     2041 caagcgcccc gaccgcttca tcggcatgca cttcttcaac cccgtgcccc tcatgtcgct
     2101 ggtcgagatc atccggggcc tcgccaccag cgacgagacg taccagacgg tgcgcgacct
     2161 tgcggtgagc ctcggcaaga cccccgtgga agtcaaggac taccctggct tcgtctccaa
     2221 ccgcatcctc atgccgatga taaacgaagc ggtattcgcg ctgttcgagg gtgtagcctc
     2281 caaagaggac atcgacaccg tgatgaagct gggcatgaac cacccgatgg gtcccctcac
     2341 gttggccgac ttcatcggcc tcgacgtatg cctcgacatc ctctacgtcc tctacgatgg
     2401 cttcaaagac cccaagtacc gcccctgccc cctgctcgta aaaatggtcc aggcaggcaa
     2461 actgggccgc aagactggag aagggttcta cacgtattga gtaatgagtg atgaggactg
     2521 aggactgagt aaatagacgg aaacgcaaag acgcaaagga cgcgtaaaga gtgggattgt
     2581 ttggagtggt gccattcgta actccgaact caatcctcag tcctcatcac tcagtcctca
     2641 ttacttccta ggaaggcaca cacatggatt tcgcgctatc ggaagaccag cggctaatca
     2701 agcagacggc cagggagttc gcggacgagc agcttgctcc cctggcggcg gagtacgacc
     2761 gcacggagga gttcaccgct ccccaggcga agatgctggc tgagatgggt ttcctcggca
     2821 tgatggtgcc ggaggagtac ggcggctccg gcctggacac gatttcgtac gtgctcgcga
     2881 tggaggaggt gagccgggcc tgcgcctcct cgtccgtgac catgactgtg caaaactcgc
     2941 tggtgtgctg gcccgtcacg gcgttcggca ccgacgagca gaagcgcaag tacctcgtgc
     3001 ccctcgccag gggtgagagt ctcggctgct acgccctgtc cgaaccggcc gcaggctccg
     3061 atcctgcctc gatggcgacg atggccgtgg aagatggcga ctcctacatc atcaacggct
     3121 ccaagatttt catctccaac ggcggcgtgt cgaaggtggc cctcgtgtgg gcgacgacca
     3181 gcaaggagct aaaacacagg ggcatcaccg ctttcatcgt ggagagcgac accccaggct
     3241 tcacggtggg ggcggaggag aaaaagctcg gcatacgcgg ctccaacacc accgagctgc
     3301 atttcgagaa catgcgcgtc tccaaggcca acatcctcgg caacgtaggc gacggtttca
     3361 agatcgccat gcagaccctc gacggcgggc gcattggcat cgcggcccag gcgctgggca
     3421 taggccaggc ctgcatcgac gcttcggtca ggtacgccca ccagaggcag gcgttcggca
     3481 agcccctcgc cgaaatagaa gcgatacaaa tcaaagtggc cgacatggcg acggaggtgc
     3541 aggcctcccg cctcctgacg ttgcatgccg ctttcatgaa ggaccagggc ctgcgcttca
     3601 gcaaagaggc cgcgatggcg aagctccacg cctccgagac cgctaccagg gtagcgcatg
     3661 cggcggtcca gatccacgga ggctacggct tcctgcggga cttcccggtc gaacgattgt
     3721 accgcgacgc tcgcatcacc gagatttacg aaggcacgtc ggagattcag cggcacgtaa
     3781 tcagcaacgc cgtcttgaga gagggccgtc ccaccgaata gacctaccta ttttgagaga
     3841 tgttgggggc catgagcacg gtacagtttg gcctggtggt cccaggtgac gcgctggaca
     3901 cgtcgaggcg acacctctac atggaggatg tgagccgcct gctcaactac gtgaagggcc
     3961 actaccactc ggcgtggttc atcgaccacc tgcagttcgg cagcaccgac gtactggaag
     4021 gctggacggc cctcgcttac ctctccgcgc ttcaccctga gctacagtgg ggacatagcg
     4081 tcatatgcca ggcattccgc aacccggcgc tactcgcaaa gatggcggcg accctgcagt
     4141 tcatgtcggg cgggcgctac attctcggca tcggtgcggg ttggcatgaa gaggagcacc
     4201 tggcttacgg ctatgacttc cccacgggca gcgccagagt cgaagagttg gacgaggcgc
     4261 tccagataat caaggcgatg tggacgcagg agagcgccac cttcgagggc aagcactacc
     4321 gcgtgaagga ggcctgttgc gagccgaagc cccaacctat ccccactatc atggtcggcg
     4381 cgttcggacc taagatgcta cgcctggccg caaggcacgc cggctggtgg aacgtgtcct
     4441 cgacccctat agacacctac cgcggctacc tgcgagagtt cgagcgggcg tgtgaagagg
     4501 tagggcgcga cgttaggacg gtacgccgct cgtggggtgg aggctgcgtg tgcgccccaa
     4561 cggagcggga agtcgccagg ctggcgtcag agcggcatca gcccgaagaa gacttcgtcg
     4621 gcacccccag gcagcttata gagcagatgc agccattcgt agagttgggt gtagactatt
     4681 tcatgctcga ctgtgggggc ttccctgagc tgactacagt agagatgctg gtcaacgagg
     4741 tgctgcccgc actcaaccgg taggcttttt agttgaagcc actaagaaat agccgctcgg
     4801 aagataggga ccaagctccc cgcttgtcat tccgagcgta gcgaggaatc aggtggcagt
     4861 cgtactcagt aggagggcag taggcgactg gcaaacgcac actggcacta ggcactggaa
     4921 tgccgcttct tccctaccca cctgattcct c
//
LOCUS       DASRWZ010000461         7387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_586631, whole genome shotgun sequence.
ACCESSION   DASRWZ010000461 DASRWZ010000000
VERSION     DASRWZ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586631"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFH60_07190"
     CDS             complement(<1..556)
                     /locus_tag="VFH60_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET6313604.1"
                     /translation="MSQQCPNCGSPVQAGHRFCSNCGYTQAPTLPVEAVQTTTPAPAS
                     TPQSDSAIDTPPVTYVVQRHDPAFPAGEAPTVPSSGLTVRPVGPEAQGQPYPAFVTPP
                     LPGQAEGTAQAEIPRGAIPPPPPPARGFDLSQVGTGSSTYGNCSAAAGPRLQGGAFAP
                     YEGGAVRQLERESSQRNWLMPVLAA"
     gene            complement(717..1517)
                     /locus_tag="VFH60_07195"
     CDS             complement(717..1517)
                     /locus_tag="VFH60_07195"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET6313605.1"
                     /translation="MGFIYTAIAFVLGLVAGWGINLVATRLAAQRPMFGPLGCTRSPH
                     PISVSQALPVLGYLAQRGKCSQCGRKLPVAFPATELATGALVAGLCWLEGFSLPFLFH
                     SAYVVVLMLVLVIDWKHRDIYFSIIILGSLIAVAGLFVLPGMDPVGGLVGAGVAGGFF
                     LLAYLLARAIFPKIEEPLGLGDVLLALMMGLMLGFPNIVGALLIGPLIAGAAAILLLV
                     SQRSKMGDFMPYGVALCAATILFIIYPAPFAEALHLPVLTYLVDGIFR"
     gene            complement(1626..3260)
                     /locus_tag="VFH60_07200"
     CDS             complement(1626..3260)
                     /locus_tag="VFH60_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313606.1"
                     /translation="MAGRLGGLLIAGVALLCALALAVASLRHTGSLAFPIDDGYIYSN
                     YVAAAAQGQFFTYNPGETSGGITGLGWYLLCLVAYGLLAPFHSLFGGLAPALVAGNGP
                     LAAQAGHLFLAAYLPGAACLAATGLGTRKLATLALPLVEGKQLSRDVLCWLLGVLAAA
                     DLGLTWGAMSGLEIALSSAVVPWALCLLLLDWRRGWPGWSLLLCALLPWARPDLLVFA
                     GVGLTWLLLRFLLTRDANLRGRAGRFLLSYLGAAAAGTGLLALTYFLGWGRPLPSSFY
                     AKVGGLRLFRGFGSAAQELLIAGRYVAFVIAALALFGGLVWLLAPTRRSPHAETRWTA
                     LLLLVSFVSYVFALMLIQPWFGQEDRYILPVHPCAIVLLGMLLWYFASALPVERLFAP
                     RFVLPVLAGAITALAVLTNYVWATRTYVVYVRNITDAHVAPARWLAQNTPPDALVASE
                     PIGAVRLFSERRTFDVVGLTSPAALGTYGAWPTMEAAMREAGAGYLLIYPVWFDGGQP
                     YDWAVERVRYSVPDNRIAGQDLIAVYELRFDAATSP"
     gene            complement(3540..4742)
                     /locus_tag="VFH60_07205"
     CDS             complement(3540..4742)
                     /locus_tag="VFH60_07205"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6313607.1"
                     /translation="MKLRLADRMARLGTETAFEVAARARALEAQGRPIIHLEIGEPDF
                     DTPTHVRQEAQRALDEGYTHYSNSTGILELREAIAEYAGRSRGLKFDPANVIVTPGAK
                     PIMFYAIMALVEEGEEVIYPNPGFPIYESMINFLGGTAVPLQLSERNDFRIDLDELER
                     KVSDKTRLIIFNSPHNPTGGVLTRDDLKAIAEFAVKHDIMVLADEIYSEIVYEGEHHT
                     ILSFPGMEERTILLDGFSKTFAMTGWRLGYGIFPPEMVPHVSRLIINSVSCTSTFSQR
                     AAIAALNGPREEVDAMLKAFGERRELVYEGLNRIPGLRCNRPAGAFYAFPNITDTGMQ
                     SRAYADYLLTEADVAVLPGTSFGAYGEGYIRISFANSADNLREALRRIEQANLRLPAL
                     KAEAGAAK"
     gene            complement(4855..5934)
                     /locus_tag="VFH60_07210"
     CDS             complement(4855..5934)
                     /locus_tag="VFH60_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET6313608.1"
                     /translation="MDELLEMFKELTEAPGVPGQEGPVRDIMARYLAPLGEVVTDKLG
                     SIVAYKSGQGVNPDSAPRVLMAGHMDEVGFLIKLIDDNGYLKFQTLGGWWEQVMLAQR
                     VLIRTHKGDIVGVFGSKPPHLLPADERKKMTQKEDMFIDIGVTSKEDAIEAGVRPGDF
                     AVPICPFTVLANENMLLAKAWDNRFGCALAIETLRQLGDDHPNTLYAGATVQEEVGLR
                     GAQTLAQMVRPDVGIALDVAIAGDTPGVKSHEAQCKMGKGPVILIYDGTLIPNLKLRN
                     LVIDTAEAEGLPYQFDSIAAGGTDGGKFQFAGDGVPSISIGVPTRYIHSAASMMHLDD
                     FNNATRLMVAVVKRLDADTVADLTR"
     gene            complement(6005..6451)
                     /locus_tag="VFH60_07215"
     CDS             complement(6005..6451)
                     /locus_tag="VFH60_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc finger domain-containing
                     protein"
                     /protein_id="HET6313609.1"
                     /translation="MAEGQSAQTYSDDQFVTYIVTDVLNFGGFFGGETVTLDAHPQSA
                     PDTFASFIIPEHLFDNLTQRHLIAPGMALGLIVDNGEVRAARVLGATTREQLKEIVKP
                     PPPSLDGAPGPRALSYRCTQCDLWVTGTPDLREGTYYCRLCGTKLG"
     gene            6723..>7387
                     /locus_tag="VFH60_07220"
     CDS             6723..>7387
                     /locus_tag="VFH60_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6313610.1"
                     /translation="MQTVTQGTGNLDPHRDLLAYRDEFPILSKKTFLNTCSLGALSRR
                     SRANVEEFLDLWDELGASAWYELWVGKLAELRQAYGRTINAAPERIALMPSISVAVAG
                     VSSGLDFSKRKKVVMSDLDFPTLGHGFLAKRQQGVHVEFVRSPDRVTVPLELFEAAID
                     EDTALVATSHVYFTSGAIQDIKTLARIAHGRGAMLMLDAYQGTGHLPTDVHDLDVDFY
                     VSG"
BASE COUNT         1402 a         2264 c         2413 g         1308 t
ORIGIN      
        1 cggcggcgag cacgggcatc agccagttcc gctgcgagct ttcccgttcc agttgccgta
       61 ccgcgccacc ctcgtagggc gcaaacgcac caccctgcaa tcttggtccc gccgcggcgg
      121 agcagttgcc gtaggtgctg cttcccgtgc ccacctgcga gaggtcgaag ccgcgcgccg
      181 gcgggggcgg aggtggaatt gcgccccttg gtatctctgc ttgtgcagtg ccttctgcct
      241 ggcccggcaa tggaggagtc acgaaggcgg gatacggctg gccctgcgct tccggaccaa
      301 ccggccgcac cgtgaggccg ctacttggga cggtcggggc ttcgccggct ggaaaggcgg
      361 gatcgtgacg ttgcaccaca taagtcaccg gcggggtgtc tattgcgctg tcgctctggg
      421 gagtgctcgc cggggcgggg gtggtagttt ggaccgcctc cacaggcagg gtcggtgcct
      481 gggtgtaccc gcagttggaa caaaatcggt gacccgcctg cacgggcgag ccacaattgg
      541 gacattgttg agacatgaaa taatcctggc ttcttagctg aaaactgagg gggaccggct
      601 tatcgtcgcg catgccgcgt aacagggctt tagaatggag tatacggcat gaaaaactgg
      661 cccgtcaaga attggccgcc cctggctatt gggcgaatac ctggctgcta cggaagttac
      721 ctgaagatgc cgtcaacgag gtaggtgagc acgggcaggt gcagggcttc cgcgaagggg
      781 gcgggataga tgatgaacag gatcgtggcg gcgcagaggg ccacgccgta gggcatgaag
      841 tcgcccatct tgctgcgctg agagacgagc aggagtatgg ccgccgcccc cgctatgagc
      901 ggcccaatca ggagcgcgcc cacgatgttg gggaagccga gcatgaggcc catcatcagc
      961 gcgagcagca cgtcgcccag gccgagcggc tcctctattt tggggaatat ggcacgcgcc
     1021 agcaggtacg ccagcagaaa gaacccgccc gcgacccccg cgcctaccag cccacccaca
     1081 gggtccatac cgggcagcac gaacagcccc gccacggcta tcagggagcc aagtatgatg
     1141 atggagaagt agatgtcgcg gtgtttccag tcgattacca gcacgagcat gagcaccacg
     1201 acgtaggcgg agtgaaacag gaagggcaag ctaaagccct caagccagca cagcccggcg
     1261 acgagcgcac ccgtcgcaag ctcggtggca gggaaggcaa ccggcagctt gcgcccgcac
     1321 tgcgagcact tgccgcgttg cgccaggtag cccagcaccg gcagcgcctg cgacacggat
     1381 atagggtggg gcgagcgcgt gcaaccgagg gggccgaaca tgggcctctg cgcggctagc
     1441 ctggtagcca cgagatttat gccccacccg gcgacaaggc cgagcacgaa ggctattgcg
     1501 gtgtagatga aacccaaatc aacttacctc gaaagtcacc gattccgaag caaatataag
     1561 ccatgcacgc gcggggcgca atgggagaat cgtttgcttg gaggcagccg aaggtcacac
     1621 tcgccttacg gagaggtagc cgcatcgaat cgcagctcgt acacggctat caagtcttgt
     1681 ccggcgatcc tgttgtcggg gaccgaatag cgcacccttt ctacggccca gtcgtagggt
     1741 tgcccgccat cgaaccagac cgggtagatc agcaggtagc cggcacctgc ctcgcgcatg
     1801 gcggcctcca tcgttggcca ggccccgtac gtccccagcg cggcggggga ggtcaacccc
     1861 accacgtcga acgtacgtct ttcagagaag aggcggacgg cccctatcgg ttcggaagct
     1921 acaagggcgt cgggtggtgt gttctgcgcc agccagcggg cgggggctac atgagcgtcc
     1981 gtgatgttgc gcacgtagac gacgtaggtg cgggtcgccc agacgtagtt ggtgagcacc
     2041 gccagcgccg ttattgcccc ggcaagcacc ggcaagacga agcgcggggc aaatagccgc
     2101 tccactggca gcgcgcttgc aaagtaccac aacagcatgc ccagcaggac aatcgcgcag
     2161 gggtgcacgg gcaggatata gcggtcctcc tgcccgaacc agggctggat gagcatgaga
     2221 gcgaacacgt agctcacgaa cgacacgagc aacaggaggg ccgtccaccg cgtttcggca
     2281 tgcggggacc tgcgtgttgg ggctagcagc cacaccagac cgccaaagag ggcaagggcc
     2341 gcgatcacga aggcgacata acgacccgct atcagaagct cttgcgccgc gctgccaaag
     2401 cccctgaaca ggcgcagacc tcccaccttt gcgtagaagg aacttgggag cggacgcccc
     2461 caaccgagga agtaggtgag cgccagcaga cccgtcccgg cagcagcggc cccaagatag
     2521 gagaggagaa agcggcccgc gcgcccgcgt agattggcgt ctcttgtgag caagaagcgt
     2581 agcagcagcc atgtcagccc cacgcccgcg aaaacaagca ggtcgggtct ggcccagggc
     2641 agcaaggcac acaggaggag actccaaccg ggccagccac gccgccaatc gagcaggagc
     2701 agacaaagcg cccacgggac cacagccgaa gacagcgcaa tctccaggcc ggacatagcg
     2761 ccccaggtga ggcccaggtc ggcggcggca agcaccccca ggagccagca cagcacgtct
     2821 cggctgagtt gcttgccctc cacgaggggg agcgccaggg tggccagttt gcgcgtgccc
     2881 aggccggttg ctgccaggca agcagcgccc ggaaggtagg ccgcgaggaa gaggtgccct
     2941 gcctgtgccg ccagcgggcc gttgcccgca actaacgcag gtgccaggcc gccaaagagg
     3001 gaatggaagg gcgcaagcaa cccataagcc accaggcaga gcaggtacca gccgaggccg
     3061 gtgatacccc cgctcgtctc gcccgggttg taggtgaaga actgcccctg ggcagcggca
     3121 gccacgtagt tgctgtagat gtatccgtcg tctatgggga aggcgagcga acccgtgtgc
     3181 cgcagcgaag ccaccgccag cgcgagcgcg cacagcagcg caaccccggc gataagcaac
     3241 ccgcccaagc gtccggccaa aaacgaccgc acctccggga actgcgccct actttctcca
     3301 ggcatacgcc gattctacca gacccgctgc cgcagaacaa cggttgaaca catcagctac
     3361 atcgtatcta aacgccaaga cgccaagagt gttgaggttt ggagagtaca aaaaagaaca
     3421 aggtactaca agatgtaccc tgttcctttt tgtactatct aaccattctt ctctttgcgc
     3481 gtcctttgcg tcctttgcgt ctttgcgttt tcgtctcttg gcgtttagtt cccttactcc
     3541 tacttcgcgg ctcctgcttc ggctttgagg gcgggtaggc ggaggttggc ttgttcaatg
     3601 cggcgcaggg cttcgcgcag gttgtcggcg gagttggcga aggagatgcg gatgtagccc
     3661 tcgccgtacg cgccgaagga ggtgccgggg agtacggcca cgtcggcctc ggtcagcagg
     3721 tagtcggcgt aggcgcgcga ctgcatgccg gtgtcggtta tgttggggaa tgcgtagaaa
     3781 gcaccggcag ggcggttgca gcgcaggccg ggtatcctgt tcaggccctc gtacacaagc
     3841 tcgcggcgct ccccgaacgc cttcagcatc gcgtccacct cttccctggg gccgttgagc
     3901 gcggctatcg ctgcacgctg gctgaaggtg gaggtgcacg acacgctgtt tataatcagg
     3961 cggctgacgt gggggaccat ctcaggcggg aagatgccgt agccgagccg ccagcccgtc
     4021 atcgcgaacg tcttggagaa gccgtccagc aggatggtgc gctcctccat gccggggaag
     4081 ctcaggatgg tgtggtgctc gccctcgtac acgatttcgc tgtaaatctc gtcggccagc
     4141 accataatgt cgtgctttac cgcgaactcg gcaatcgcct tcaggtcgtc cctggtgagg
     4201 acgccccccg tgggattgtg gggcgagttg aagataatca ggcgggtctt gtcggagact
     4261 ttgcgctcaa gctcatcgag gtctatgcgg aagtcattgc gctcgctgag ttgcaggggc
     4321 accgccgtcc cgcccaggaa gttgatcatc gactcgtaga tggggaagcc ggggttggga
     4381 tagattactt cctcgccctc ctccaccagc gccatgatgg cgtagaacat gatcggcttg
     4441 gcaccgggcg tgactatcac gttagccggg tcgaacttga ggccgcgact cctccctgcg
     4501 tattcggcta tcgcctcgcg tagctccaga atgcccgtgg agttgctgta gtgagtgtag
     4561 ccctcatcca gcgcgcgctg agcctcctgg cgcacgtggg tgggggtatc aaagtccggc
     4621 tcgccaatct ccaggtggat gatcggcctg ccctgcgctt ctagcgccct ggcacgcgcg
     4681 gccacttcaa aggccgtctc cgtccccagg cgcgccattc tatccgcgag tctcaacttc
     4741 atcgttgtcc tcccattcag taatgagtaa tgagtgatga ggactgagtg aagagggctt
     4801 agtcgctttc gcgtaactca gtcctcagtc ctcatcactc attactcgaa agtcctatcg
     4861 tgtcaggtcc gctaccgtgt cggcgtcgag gcgcttgacc acggctacca tcaggcgggt
     4921 ggcgttgttg aagtcatcca ggtgcatcat ggaggcagcg gagtggatgt agcgggtggg
     4981 cacgccgatg ctgatggagg gcacgccgtc gcccgcgaac tggaacttac cgccgtcggt
     5041 gccgcccgcc gcgatgctgt cgaactggta gggtaagcct tcggcctcgg cggtatcgat
     5101 gacaaggttg cgcagcttca ggttggggat gagggtgccg tcgtaaatca ggatgacggg
     5161 acctttgccc atcttgcact gcgcctcgtg cgacttcacg cccggcgtgt cgcccgcgat
     5221 tgcgacgtcc agcgcgatgc ccacgtcggg gcgcaccatc tgggccagcg tctgcgcgcc
     5281 acgaaggccc acttcctcct gcacggtcgc gcccgcgtag agggtgttgg ggtggtcgtc
     5341 gcccagttgc ctcaacgtct cgatggcgag cgcgcagccg aagcggttgt cccaggcctt
     5401 ggcgagcagc atgttctcgt tggcgagcac cgtgaacggg cagatcggca ccgcgaaatc
     5461 gcccggacgc acacccgcct ctatcgcgtc ttccttgctg gtgacgccga tatcgatgaa
     5521 catgtcttcc ttctgcgtca tcttcttgcg ctcgtcggcg ggcaggaggt ggggcggctt
     5581 ggagccgaac acacccacta tgtcgccctt gtgggtcctg atgagcacgc gctgggccag
     5641 catgacctgt tcccaccagc cgccgagggt ctggaatttg aggtagccgt tgtcgtcgat
     5701 cagcttgatg aggaagccga cctcgtccat gtgcccggcc atgagcacgc gtggcgcgct
     5761 gtcggggttt acgccctgcc cgctcttata agccacaatc gagccgagct tgtcggtgac
     5821 aacctccccg aggggggcga ggtaccgcgc catgatgtcg cgcaccggcc cctcctgtcc
     5881 tggcactccg ggggcttcgg ttagctcctt gaacatctcc aacagttcat ccatagttcg
     5941 gtagggtcct ccgtggtagt gttatagcgc gtgggcgatt cgcctggcct gaagccgagt
     6001 ctggctaacc cagcttggtg ccgcaaagac ggcagtagta tgttccttcg cgcaaatcgg
     6061 gcgtgcccgt gacccagagg tcgcactgag tgcagcgata ggagagggcg cgcgggccgg
     6121 gggcaccgtc gagcgagggg ggcgggggct tgacgatctc tttcaactgc tcgcgggtgg
     6181 tagcgccgag cacgcgggcc gcccgtacct cgccgttgtc cacgatgagg cccagggcca
     6241 taccgggggc gatgaggtgg cgctgggtga ggttgtcgaa aaggtgttcg gggatgatga
     6301 agctggcgaa cgtgtcgggg gccgactggg ggtgagcgtc gagcgtcacc gtttcgcctc
     6361 cgaagaagcc gccgaagttg agcacgtcgg tcactatgta ggtgacgaac tggtcgtccg
     6421 agtaagtctg cgctgactga ccctcagcca tcaataatct cctcgcgtgg ggtgtgcggt
     6481 caaacagtag ctacgtatta tatcatacag ggctaaagcg tatgagttgt gagcagcgct
     6541 gtatcacgca gggcattcca atcagcaagg gagtgtgttg ctcgcccggt ttggctgtag
     6601 catcacctcc cacagggcac ccccacgggc gacgttcacc ggatctggtc aatctagcta
     6661 gattttcgct tcagtgttat atttggagga ctatccaact gacctaacgc ggagtgttac
     6721 ccatgcaaac cgttacccag ggcaccggca acctcgaccc gcaccgcgac ctgcttgcct
     6781 accgcgacga gtttcccatc ctgagcaaga agaccttcct caacacctgc tccctgggcg
     6841 cgctctcccg gcgctcacgg gccaacgtgg aggagtttct cgacctgtgg gacgagctgg
     6901 gggcctcggc ctggtacgag ctttgggtgg gcaagctggc ggagctgagg caggcgtacg
     6961 ggcggaccat caacgccgcg cccgaacgca tcgccctcat gccctccatc tcggtggccg
     7021 tggcgggggt gtccagcggg ttggacttca gcaagcgcaa gaaggtggtc atgtccgacc
     7081 tcgacttccc cacgctggga cacggcttcc tcgccaagcg gcagcagggc gtgcacgtcg
     7141 agttcgtgcg cagccccgac cgcgtgacgg tgcccctgga gctgtttgag gcggcgattg
     7201 acgaggatac ggccctcgtg gcgacctccc acgtctattt caccagcggg gcgatccagg
     7261 acatcaagac gctggcgcgc atagcccacg ggcggggcgc catgctcatg ctggacgctt
     7321 accagggcac cggccacctg ccgaccgacg tgcacgacct cgatgtggac ttctacgtgt
     7381 cgggtag
//
LOCUS       DASRWZ010000462         4677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_517752, whole genome shotgun sequence.
ACCESSION   DASRWZ010000462 DASRWZ010000000
VERSION     DASRWZ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517752"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFH60_07225"
     CDS             complement(<1..962)
                     /locus_tag="VFH60_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313611.1"
                     /translation="MGNVIIARSWRSYKQVVRSFLYRVYYILFRAGPPGERLCLYQGF
                     YYLLRNWLRRHGNRGLLARSRTWPHVKISLFFFGVLFVIPGICNLINGTLLSKNVQTS
                     TVGTPVRVLGFLEDWGIVLIATVLWPAHAAVMYGVLTRVSNALRDLTRSKILLINASD
                     YSKFVRNYNDWANAGWLAFGIILVSAIYTGYVYASRQGAWWGQLQYGLLGFMAYLWLT
                     FITIHQIVMYVCKGLAGVLLTWDVLTRRRVVLDLQPYHQDGVHGIAELGRIQFLIWVG
                     NFILTIFLFVLPASGVVELKPGFILLFAVVYLIILPVVAYFPVR"
     gene            complement(1144..2046)
                     /locus_tag="VFH60_07230"
     CDS             complement(1144..2046)
                     /locus_tag="VFH60_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HET6313612.1"
                     /translation="MPLHTAEGHVKPHPPFDFDKSKEFLAGFAPTRRDHTPGAEAFTR
                     ALSIEGQVVAFQLTSTGSVQEPELHYRLLSEQPLGSALQHRVLDRIALYLSLHDDLGP
                     FYRLGREDPLFAPVVERLYGYHQVKFLSPFEAGCWAVLTQRNAMSEARKMRVVLVKRY
                     GGSIEVEGATHWAFPEPTQLAVADPSDLGAITDNERKGEYMQALARAFDQVSDEFLLS
                     APYAEVEAWLRGIKGVGEWSAAFILLRGLGRPDRVPMEKNTLRAISRVYGQGKALSEG
                     EAMEIAQRYGAWQGYWAHYLRAAI"
     gene            complement(2128..4143)
                     /locus_tag="VFH60_07235"
     CDS             complement(2128..4143)
                     /locus_tag="VFH60_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET6313613.1"
                     /translation="MILLFVGGLGYGVYNLTGQSFGAPGTGSTAQAPAIDEWPAYMQN
                     GARTGFNAGETRLSPANAAKLKLLWKAQLGNVLAAQPIVKDGVVYAGSWNGFLYAFNT
                     KDGSQLWKKDLGRTTSKRCVPDTAGISSAPAVTDDALYIGGGDEYFYALNPKTGEELW
                     KFKTGDNSEAGGAYNWASPIVHGGRVYTGIASFCDRPFAHGEMWGLNTATGAVEQQVS
                     FVPQDQRGGGIWTSPTVDEATGDFYVTTGSGDYYIQHSYSLARLNPSTLDVEDAWQIP
                     IEVQVFDGDWGTTPTLFRHRDGRLMSGAMAKNGYYYAFDTADMRSGPAWSMKIGDGGS
                     CPQCGDSMISSSAYAYDTVFAASGYISLGQAQKFAGMVYALDPSTGAVRWSHPTQGSI
                     FAALAVANGLLVAAPDDTVEVLDAATGKLLWEYATGGVLYAAPTIAEGTLYVASTDGY
                     LYAFSAGPYSENTQPYRVETVGSNPPGFTPFRTPVAAPKLQGDEQCFRDTGHCVRGGF
                     LDFWRDNGGLDRLGPAVTGELSEAGRTVQYFRNVVLEIGPDLDGSGPRVRFGRLDFRL
                     FYPTPTNEAFDPADPASGAAYVPETSHNLSEPFLSYWRARGEVPGLGYPVSEPFDEYS
                     VLDDTVRRVQYFERARLELVKGADGTEQVVMGALGLQRYKQRYGTLP"
     gene            complement(4444..>4677)
                     /locus_tag="VFH60_07240"
     CDS             complement(4444..>4677)
                     /locus_tag="VFH60_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6313614.1"
                     /translation="QMLKEQVEDILSKLTERERKILEMRYGLADDRPRTLEEVAREFN
                     ITRERIRQIETRMRRTLRAARYGASKLKGYLDY"
BASE COUNT          873 a         1406 c         1467 g          931 t
ORIGIN      
        1 aacctgaccg ggaaataagc gacgacgggg aggataatca ggtacacaac cgcaaatagg
       61 aggatgaatc ccggcttcag ttctactaca cctgatgcag gcagtacgaa caagaagata
      121 gttagtatga agttgccgac ccaaattaga aactggattc tgcctaactc ggctatccca
      181 tgtacgccgt cctgatggta cggttgaaga tcgagcacga ctcttcttcg ggtaagcaca
      241 tcccatgtga gtaggacacc cgccagtccc ttacagacgt acatgacgat ctggtggata
      301 gtaatgaagg tcaaccataa ataggccatg aagccaagca gtccatattg caattgcccc
      361 caccacgcgc cctgtctact ggcatatacg tacccggtat agattgcgga tacaaggata
      421 atcccgaacg ccagccaccc agcattggcc cagtcgttgt agttgcggac gaacttactg
      481 taatctgagg cattaattag caggatttta gagcgtgtaa ggtctctcag ggcgttcgat
      541 acgcgagtga gcacgccata catcacagca gcgtgtgccg gccaaagtac cgtggcaatc
      601 agtacgatac cccagtcctc caggaaaccc aataccctca cgggggtccc tacggtagat
      661 gtctgaacgt tcttggacag cagtgtgcca ttaattaagt tacagatccc cggaatgacg
      721 aacaggactc caaagaagaa aagcgatatc ttgacgtggg gccaagttct ggacctggct
      781 aaaaggcccc gattgccatg cctgcgcagc cagtttctga gcagatagta gaaaccttga
      841 tacaaacata ggcgctcccc tggaggcccc gccctgaaaa gtatgtaata taccctgtat
      901 agaaatgaac gtacgacctg tttgtatgac cgccaactac gggcgatgat gacattgccc
      961 atcctgatgg tcctcctcat acggtagtca ctatcccgta ttgtggtcca ttcacggttc
     1021 aggctttact aatgcccagg ttgctgaact ggttagaata ttattagtcg ctactataac
     1081 acataattta tgcatatatt gtcaacattt gacaacagat cacgtcaata ggtaatgtag
     1141 ccctcatatg gcagcacgga ggtagtgcgc ccaatacccc tgccacgccc cgtacctctg
     1201 cgcgatctcc attgcctccc cttcgctcaa agctttaccc tgcccgtaca cccgcgaaat
     1261 agccctcagc gtgttcttct ccatcggcac gcggtcgggc cgccccaggc cgcgcaggag
     1321 gatgaaggcc gccgaccact cgcccacccc cttgatgccg cgcagccagg cctccacctc
     1381 ggcgtagggt gccgacagca ggaactcgtc gcttacctgg tcgaaggcgc gggcgagggc
     1441 ttgcatgtac tcgcctttgc gctcgttgtc ggtgatagcg cccaggtccg aggggtcggc
     1501 tacggcaagt tgggtcggtt cgggaaaggc ccagtgggtc gcgccctcga cctctatgct
     1561 gccgccgtag cgcttcacca gcactacgcg catcttgcgg gcctccgaca ttgcgttgcg
     1621 ctgggtcagc accgcccagc accccgcctc gaagggcgac aggaacttca cctggtggta
     1681 gccgtacagc ctctcaacga ccggggcgaa caaggggtcc tcgcggccca gcctgtagaa
     1741 cggccccagg tcgtcgtgca ggctgaggta gagggctatg cggtccagca ccctgtgttg
     1801 caaggcgcta cccagcggct gctcggacag gagcctgtaa tgtagctccg gctcctggac
     1861 gctgccggtg gaggttagct gaaacgctac cacttgcccc tctatggaga gcgccctcgt
     1921 aaacgcctcc gcgccgggtg tgtggtccct ccgggtggga gcgaagcctg caaggaattc
     1981 tttcgatttg tcgaagtcga acggtgggtg tggtttgacg tgcccttctg cggtatgcaa
     2041 tggcaagcgc gttacctctc tgcggcgtta tgcgcggcgt tgctgtctgt gccgcgaagc
     2101 tacggggacc gctcgtcctg gagttgacta ggggagcgtg ccgtatcgct gcttgtacct
     2161 ttgcaggccg agcgcgccca tgaccacctg ctctgtcccg tccgccccct tcaccagttc
     2221 cagccgcgcc cgctcgaagt actgcacccg gcgcaccgtg tcgtccagca cgctgtattc
     2281 atcgaacggc tcgctgaccg ggtagccgag gccgggcacc tcgccgcgag cgcgccagta
     2341 ggagaggaac ggctcggaga ggttgtggct cgtctccggc acgtatgcag cgccggatgc
     2401 tgggtccgcc gggtcgaatg cctcgttcgt gggcgtgggg tagaagaggc ggaagtcgag
     2461 cctgccgaac ctgacgcggg ggccgctgcc gtccaggtcg gggcctatct ccagcacgac
     2521 gttgcggaag tactgcacgg tgcgcccggc ctcgctgagt tcaccggtga cggcggggcc
     2581 aagccggtca agcccgccgt tgtcgcgcca gaagtcgagg aagcccccgc gcacgcaatg
     2641 gccggtatcc cggaagcact gctcgtcgcc ctggagctta ggggcggcta cgggggtgcg
     2701 gaacggggtg aagccgggag ggttgctgcc caccgtctcg accctgtacg gttgtgtatt
     2761 ctcgctatat ggccccgcgc tgaaggcata gaggtagccg tcggtggacg cgacgtagag
     2821 ggtgccctcc gctatcgtgg gcgcggcgta gagcaccccg ccggtggcgt actcccacag
     2881 cagcttgccc gtggcggcgt ccagcacctc caccgtgtcg tcgggggcgg ctaccagcag
     2941 cccgtttgcc actgccagcg ccgcgaagat ggagccttgc gtcgggtgcg accatcggac
     3001 ggccccggtg gaggggtcga gcgcgtacac catgcccgcg aacttctgcg cctgaccgag
     3061 cgagatgtag ccgctggccg cgaacaccgt gtcgtaggcg taggcgctgg aggagatcat
     3121 cgagtcgccg cactgcggac aggagccgcc gtcgcctatc ttcatgctcc acgccgggcc
     3181 actacgcatg tcagcggtat cgaaggcgta atagtagccg ttcttcgcca tcgcgcccga
     3241 catgagcctg ccgtcgcggt gcctgaacag ggtgggggtg gtgccccagt cgccgtcgaa
     3301 cacctgcacc tcgataggta tctgccaggc gtcctccacg tcgagggtgc tggggttgag
     3361 gcgggcgagg ctgtagctgt gctggatgta gtagtcgccg gagccggtgg tcacgtagaa
     3421 gtcgcccgta gcttcgtcca cggtgggcga ggtccagatg ccgccgccgc gctggtcctg
     3481 cggcacgaag ctcacctgct gctcgaccgc gcccgtggcg gtgttgagac cccacatctc
     3541 gccgtgggcg aagggcctgt cgcagaagga ggcgatgccg gtgtacacgc ggccgccgtg
     3601 cacgatgggc gaggcccagt tgtacgcgcc acccgcctcg ctgttgtcgc cagtcttgaa
     3661 cttccaaagc tcctctcccg tcttggggtt gagggcgtag aagtactcgt cgccgccgcc
     3721 tatgtagagg gcgtcgtcgg tgacggcggg ggccgagcta atcccggcgg tgtcgggcac
     3781 gcagcgtttg gaggtggtgc gccccaggtc tttcttccag agctgggagc cgtccttcgt
     3841 attgaaggcg tacaggaagc cgttccagga gcctgcgtac accacgccat ctttgacgat
     3901 aggctgggcg gccaggacgt tgccaagctg ggccttccag aggagcttga gcttggcggc
     3961 gttcgccggg gagaggcgcg tttcgcccgc gttgaagccg gtgcgcgccc cgttctgcat
     4021 gtaggcgggc cactcgtcta tagcgggtgc ctgggcggtg ctgccggtgc cgggtgcgcc
     4081 gaaactttgc ccggttagat tatagacccc gtaccccagg cccccgacaa acagtaatat
     4141 caagaggaat agtaggggcc gccggtatct tgctcgtcgt cttgatcttt ccatgttgct
     4201 tgtttgtttc taatgggatt gaagctgtgg agggtggccg tcgcgctgag agggtaatga
     4261 gcaacttgtg cttagagggc acaaaacaca agagtgggag tttgccctgc caatgaattg
     4321 gcgggcttac aagagacgaa ggagacttcg tctcttgtaa gcccgtggct gcagccacag
     4381 ggcaatacgg cagggcgtcg ccttactacc tgccgcctca ccactgagaa cgattaagcc
     4441 gtgctagtag tccaggtagc ctttgagctt gctcgcaccg tagcgggcgg cgcgcagcgt
     4501 gcggcgcatg cgggtctcta tctggcggat gcgctcgcgg gtgatgttga actcgcgggc
     4561 cacctcttcg agcgtgcggg ggcggtcgtc ggccaggccg tagcgcatct caaggatctt
     4621 gcgctcgcgc tcggtcagct tgctcaggat gtcttccacc tgctccttga gcatctg
//
LOCUS       DASRWZ010000463         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1203263, whole genome shotgun sequence.
ACCESSION   DASRWZ010000463 DASRWZ010000000
VERSION     DASRWZ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203263"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1017
                     /locus_tag="VFH60_07245"
     CDS             301..1017
                     /locus_tag="VFH60_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6313615.1"
                     /translation="MRNNGAGDEKERPARVLVVDDEEIIVEMLTMGLNYEGFEVSVAR
                     TGLEALEQARSAKPDIVVLDVMLPGIDGVEVCRRLRAAGNVGILMLTARGEVEDRVMG
                     LDSGADDYLVKPFTFRELLARVGAILRRKGVNLQQALRVGDLVLDRQTRRVTRGSRAI
                     ELTPREFDMLELFLSHPRQVFSREVILNRVWGYDYFGDTNVIDVHIRHLREKLDDENR
                     DLIRSVRGVGYSLEPPDVTG"
     gene            1004..2536
                     /locus_tag="VFH60_07250"
     CDS             1004..2536
                     /locus_tag="VFH60_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6313616.1"
                     /translation="MSPARRASGLVWNVPVGLQLSVIYALLLAAVLTLLGFALYAQLD
                     SFLIQNTRERLERTTSTTLQQLFPQRDRRPPRTLESVTESLVRGLTSPDVVVAVLDDQ
                     GHLLSSSQSPAGGDPPYIPPLATDWHDRVANNTITQWVADAPSGGRNLVVLTPLTLIT
                     GPDAPGTLLLLQQVASLESADAILNQLRLYLALGILIGTTVGVVAGLALTRVVLRPLD
                     RISRTAEAISAGELDRRLHLPPGRNEVARLGHAFDSMVDRLASALEAQRRFVADASHE
                     LRTPLTSLEGLSEMLLIGADRGDTRVVQRTVRSMHGELRRLGRLVADLLTLSRIDSTT
                     PTITLKAVDPCALLAGIVEQMTPVAEAKGVRLSMRCDAGAEAAVLRADPDRLKQVLLN
                     LLDNALRYTPAEGEVRVWAAADVSGAWLQIGVQDTGQGIAPEDLPHVFDRFYRGDLSR
                     ARATGNTGLGLSIARAIVQSHGGSISVQSSPGQGAFFKIVLPMTKAQPAPEPEAVLAG
                     SR"
BASE COUNT          508 a          852 c          881 g          458 t
ORIGIN      
        1 catttcgaac gcagtgagaa atctcgtccc ccagcaccca aaatccctat cacccaggcg
       61 ccatctaatt atgaagaggc aaaaaaggta acatccaggt agaggacgag atttctcact
      121 acgttcgaaa tgacaagggg ccacccttgg gtgccgttct ccccaccctt attcttgaga
      181 tggcttctag taaccagtga tgagggctga ggactgagtg ttggctgggt aggtatggtg
      241 ttatagtatt ctcacaggca gaaggacaca gaggatagcg agatgcaagg agcaatgata
      301 atgcgtaata atggggccgg agacgagaag gagcgccccg cgcgcgtgct ggtggtggac
      361 gacgaggaga ttatcgttga gatgctcact atgggtctca attacgaggg cttcgaggtg
      421 agcgtggccc gcaccggcct cgaggcgttg gagcaggccc gctcggccaa gcccgacatc
      481 gtggtgctcg atgtgatgct ccccggcatc gatggggtgg aggtgtgcag gcgcttgcgg
      541 gccgccggga acgtgggcat cctcatgctc accgccaggg gcgaggtgga ggaccgcgtg
      601 atgggtctcg acagcggagc ggacgattac ctcgtaaagc ccttcacctt ccgcgagctg
      661 ctggcccgcg tgggggctat cctgcggcgc aaaggcgtca acctgcaaca ggccctgcgc
      721 gtgggcgacc tggtgctgga ccggcagacg cggcgcgtca ctcgcgggag ccgcgctatc
      781 gagcttaccc cgcgcgagtt cgacatgctg gagctgttcc tgtcccaccc ccggcaggtg
      841 ttcagccgcg aggtgatcct caatcgcgtg tggggctacg actattttgg cgataccaat
      901 gtgatagacg tgcacataag gcacctgcgc gagaagctgg acgacgagaa ccgcgacctc
      961 attcgcagcg tgcgcggtgt gggttacagc ctggagccgc ccgatgtcac cggctagacg
     1021 tgccagtggc ctggtgtgga acgtgccggt cggcctgcaa ctcagcgtca tttacgcgct
     1081 gctgctggcc gccgtgctca cgttgctggg cttcgcactc tacgcgcaac tcgacagctt
     1141 cctcatacag aacacccgcg agcgcctgga gaggaccacc agcacgacct tgcagcagct
     1201 cttcccacag agggaccgcc gcccgccccg cacgctcgaa tcggtaaccg agtccctggt
     1261 gcgcgggttg accagccctg acgtagtcgt ggctgtgctg gacgaccagg ggcacctcct
     1321 tagctcaagc caatctccgg ccgggggcga cccgccttac ataccgccac tggccaccga
     1381 ctggcacgac agggtagcga acaacaccat cacccagtgg gtagcagatg cgccttccgg
     1441 gggccgcaac ctcgtcgtgc tcacccccct cacgctgatt acaggaccgg acgcccccgg
     1501 cacgctcctg ctgttgcagc aagtggcgtc gttggagtcg gcggacgcga tactcaacca
     1561 gttgcggctg tacctggcgc tggggattct catcgggacg acggtgggcg tggtagcggg
     1621 gctggccctc acccgcgtgg tgctgaggcc gctggaccgc atttcgcgca ccgccgaagc
     1681 gatctcggcg ggcgagctgg accgccgcct gcacctccca cccggccgca acgaggtagc
     1741 ccgcctgggg cacgccttcg acagcatggt ggaccgcctg gcatcggccc tggaggcgca
     1801 gcgccgcttc gtagccgacg cctcccacga gctacgcacg cccctgacct cgctggaggg
     1861 gctgagcgag atgctgctca tcggcgccga ccggggcgac accagggtgg tgcagcgcac
     1921 ggtgcgctcc atgcacggcg agcttagacg cctgggccgc ctggtagccg acctgctgac
     1981 tctctcgcgc atcgatagca ccactccaac tataaccctc aaggcggtgg acccctgcgc
     2041 cttgctggca ggcatcgttg agcagatgac cccggtggcg gaggccaaag gggtgcgcct
     2101 atccatgcgc tgcgatgccg gggcggaagc cgccgtgttg cgcgccgacc ccgacaggct
     2161 gaagcaagtc ctgctcaacc tgctggacaa cgccctgcgc tacacgcccg ccgagggtga
     2221 ggtccgggtg tgggcggccg ccgacgtctc gggggcatgg ctgcaaatcg gcgtgcagga
     2281 caccggccag ggcatcgcgc ctgaggacct gccgcacgtg ttcgacaggt tctaccgggg
     2341 cgacctttcg agagcgcgcg ccaccggcaa cacggggctg ggcctctcca tcgcccgcgc
     2401 catcgtccag agccacgggg gcagcatcag cgtgcagtcc tcgccgggcc agggggcctt
     2461 cttcaagatc gtgctcccca tgacgaaagc gcagcccgcc cccgaacctg aagccgtgct
     2521 tgccgggagc cgctagtact gaacatagac gcaaacgcaa agacgcaaag gacgcgcaaa
     2581 gtttaagatg aggtgagtac aacgacgtaa gttagaccgg aggtcaaggg tagtgctgtc
     2641 gataaaagaa aagttcccat gggtattcct taagtgggaa cttttctttt gggtaggga
//
LOCUS       DASRWZ010000464         5724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_381880, whole genome shotgun sequence.
ACCESSION   DASRWZ010000464 DASRWZ010000000
VERSION     DASRWZ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381880"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..657
                     /locus_tag="VFH60_07255"
     CDS             277..657
                     /locus_tag="VFH60_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313617.1"
                     /translation="MTTFLIEAHRTLSNMIGIYAVVVGIWGIVNAVRRMPPDGNFNGA
                     LVLAVALFVVEGIIGMVLVLMGLRPGRIIHFLYGVTIVITIPAVFAFSRGRNTSRESL
                     FYGMAMLFIAGLSDRAASTALAGD"
     gene            657..1760
                     /locus_tag="VFH60_07260"
     CDS             657..1760
                     /locus_tag="VFH60_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313618.1"
                     /translation="MDAAHVRATPSAQDELSLLARYAASALALVGGVEWLLGRTISRM
                     AAAPTLEGTPRQIIESVGRVGLELISPTAVLALAVLLLLVVQTGASAIRLRDTAKLGV
                     SLFLAVFGVLALAHTFFPTLLWLNVTLNLLTLVALWCVAVLCLVERKRTVWMRAGVTL
                     VALAYSGWLYYLLQQATVDRGVSLPGAPLLFLNLGEIAAVLVPVAIFLAVAVPYGQWR
                     HPLRWVLPVLLAAIFAVGNVADALLNQGFMGVFTIWSVGLELFLPWPLYAISGALYVY
                     AVLTCFKGRGRRSREANPGTGLGLLLLTFAGYALQLPFQFTMAVLSIALLSGLVHISP
                     APLRPRVAGMRENIASGATQPAGAGSGPAQGPV"
     gene            complement(1765..2199)
                     /locus_tag="VFH60_07265"
     CDS             complement(1765..2199)
                     /locus_tag="VFH60_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET6313619.1"
                     /translation="MAQSDYRAYICCGPNCGPKGSARLLTLLEEEVAGQGMSERVAVL
                     PTGCQSHCETGPTMVVYPGPVYYQEIDPGRLARIVREHFGLGVPVKEYFWTGPYPKRL
                     NVLDRNAYKASNAPLEQRPGPRDTAGKPKAPRGRADVDDYKW"
     gene            complement(2290..3336)
                     /locus_tag="VFH60_07270"
     CDS             complement(2290..3336)
                     /locus_tag="VFH60_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET6313620.1"
                     /translation="MVATARPERNEKVNLIGLEKGDYKGLPSTLCQGCGHDSISSQII
                     NVAFELSLKPYEVIKMSGIGCSSKSPAYFLNRSHGFNALHGRMPSVVTGASLANNSLL
                     PLAVSGDGDTGSIGLGQYKHLLRRNVPMVYIIENNGVYGLTKGQFSATADMGQQLKYA
                     GTNELPPIDLCLEAIVLDCGFVARSFAGDAKQVRELLKAAITYNGTAVLDIISPCVTF
                     NNHEESTKSYPYGKAHEERLHDITFVPAFEPISVDYEPGTTIEVPMHDGPTIHLKKLE
                     RDYDPRDKVAALTVLTEAQQKQEFITGLIYINEGRGTLHDTAHVSGTPLAQLREEQLR
                     PSRESLDKLMAAMS"
     gene            complement(3338..5176)
                     /locus_tag="VFH60_07275"
     CDS             complement(3338..5176)
                     /locus_tag="VFH60_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET6313621.1"
                     /translation="MGTLDVLEQPASQAQDRPDEIVNDFSIVVATVNGSGSQTANGLL
                     ARAAFKMGIPVSAKNLFPSNISGLPTWYTIRLSKDGYTARRESAEVLVAFNPATAQED
                     YAALPPGGIVLHNEELKFLNLRDDVVSYPLPVKELMKQATIDPKLRDHVANMVYVGGL
                     VHLLGIDLAEIEEGLNHHFKGKAKAVDMNMNVVRLTLEWANENLPKRDPYRMERMNGN
                     QGLIMIDGNTAGGLGAIFGGVSVVSWYPITPSTSLVDALAAYIGKLRPPAEDGSETYA
                     IIQAEDELAAIGMVLGAGWAGARAMTSTSGPGISLMTEFAGLGYFAEIPGVIWDIQRV
                     GPSTGLPTRTSQADLLSTYFLGHGDTRQVVLLPGSVSDCFEFGWRAFDIAERLQTPVF
                     VLSDLDLGMNIWMTEPFEYPDQPMDRGKVLNEEQLAQVESWARYADVDGDAIPYRTLP
                     GNRHPASAYFTRGTGHNERAVYSERPDDWERNLLRLERKHDTARQYMPKPRIDIREGA
                     EIGIIAYGSTDPAIQEARDQMRKQGLETSYLQVRALPFEDTLRDFVSKHKRIYVVELN
                     AEAQLRSLLRLEIPERSMDFRSVAHLDGLPLTATYVRKAILEMEQK"
     gene            complement(5229..5537)
                     /locus_tag="VFH60_07280"
     CDS             complement(5229..5537)
                     /locus_tag="VFH60_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313622.1"
                     /translation="MTGQHQEETPQSPEAQPEYDREELDQDLVTHVGEYRQPEAGGPT
                     GGGMHLGAVDTEVVPIIPPMSGPADLLGERNQNAQGNEDGTTEIGEELIDPRDEITPG
                     "
BASE COUNT          897 a         1753 c         1839 g         1235 t
ORIGIN      
        1 cctgcgtggt agccctgcgg gggccgccct gccactaccg tggcgggctt accagagacg
       61 aagtcccctc cggagactac atgatgttcc ttagtccccg taggggactt tgcttcttgt
      121 aagcccgtgg cttcagccac agggcacccc cacagggctt gcgcagggtg tcacgtaggg
      181 cgaacgcggt acgaccacct ctctcccggc acctcaatcg ggcgctgcgg acttgctacg
      241 ttgacacgca ggcggctcta tggtaagaat ggctgagtga ctaccttcct tatcgaggcg
      301 catcgcacgc tgtccaacat gatcggcatc tatgccgtgg tcgtgggtat atggggcatc
      361 gtgaatgccg tgcgccgcat gccccctgac ggcaacttca atggtgccct ggtcctcgct
      421 gtggccctgt tcgtggtgga ggggattatc ggcatggtcc tggtgctgat gggcttgcgc
      481 cccggaagga ttatccactt cctctatggt gtgacaatcg tgattaccat ccccgccgtg
      541 ttcgcgttct cgcgggggcg caatacgagc cgcgaatccc tattctatgg catggcgatg
      601 ctcttcatcg cggggctgtc cgaccgggcg gcgagcaccg cgcttgcagg cgactgatgg
      661 acgccgcgca cgtgcgggcg acaccctccg cccaggacga gctttccctg ctggcccgtt
      721 acgccgcctc cgcactggcc ctggtaggcg gcgtggaatg gctgttggga cgcaccattt
      781 cccgaatggc ggctgcccct acgttggaag gcactcccag gcagataatc gagtcggtgg
      841 gccgggtcgg cctcgaactt atctcgccca ctgccgttct cgcgctggcg gtgctcctgc
      901 tactagtcgt tcagaccggg gcgtctgcca tccgcctgcg cgacaccgcg aagctaggag
      961 tgagcctctt ccttgcagtt ttcggtgtcc tggccctggc acacactttc tttccgactc
     1021 tcctttggct taatgtcacc ctcaacctgc tcactctggt ggcgctatgg tgtgtcgctg
     1081 tactgtgtct ggtcgaacgg aaacggacag tgtggatgcg cgccggggtc accctggtag
     1141 ccctggctta ttccgggtgg ctctactacc tcttgcaaca ggctacggtg gacaggggag
     1201 tgtcgctgcc gggtgccccg ctattgtttt tgaacctggg cgaaatcgcg gccgtgctgg
     1261 tccccgtggc gatcttcctg gcggtggcag tcccgtatgg tcagtggcgg cacccattac
     1321 gttgggtgct gcccgtgctg ctcgcagcca tatttgcggt gggaaacgtg gcggacgcgc
     1381 tgctgaacca gggcttcatg ggggtgttca ccatctggtc ggtgggcctc gaactgttcc
     1441 tgccctggcc gctctacgcc atttcgggtg ccctgtacgt ctatgccgtg ctgacgtgct
     1501 tcaaggggcg gggccgcagg tcgcgggagg cgaatccggg taccgggttg ggacttctgc
     1561 tccttacgtt cgcgggctat gccttgcaat tgcctttcca gttcaccatg gccgtactga
     1621 gcatagcgtt gctgtcgggg ttggtacaca tttcaccggc accgctccga cctcgcgtgg
     1681 ccggtatgag agagaacatc gcgtctggcg ctacgcagcc cgcaggcgca ggctccggtc
     1741 ctgcccaggg tcctgtttag aggttcacca cttgtagtcg tctacgtccg cgcgcccacg
     1801 cggggctttg ggtttgcctg cggtgtcacg ggggccgggc cgctgctcca gcggtgcatt
     1861 gctagccttg taagcgttgc ggtctaacac gttgaggcgc ttggggtaag gcccggtcca
     1921 gaagtactct ttgaccggca cgccgagccc aaaatgctcg cgcacgatcc tggccagccg
     1981 accggggtct atctcctggt agtagaccgg gccgggatag accaccatag tgggtcctgt
     2041 ctcgcagtgg ctctggcagc cggtcggcag cactgcgacc cgctccgaca tgccctggcc
     2101 cgcgacttct tcttcgagca gggtgagcag ccgcgccgag cctttaggcc cgcaattcgg
     2161 tccgcaacag atatatgccc tgtagtcgct ttgtgccatt accaatagtg cctatcttaa
     2221 ggataaaggg cggcccagca actgcgggac cgccctggct aagtttcaca acccggaaat
     2281 gcccgggagc taggacatcg cagccatcag cttgtcgagg ctctccctgg aagggcggag
     2341 ctgctcctct ctaagctgcg ccaggggcgt gccgctcacg tgcgcagtgt cgtgcagggt
     2401 tccgcggccc tcgttgatgt agataagccc tgtgatgaac tcctgcttct gctgggcctc
     2461 ggtcaggacg gtgagggcgg cgaccttgtc cctggggtcg tagtccctct ccagcttctt
     2521 caagtggatg gtcggcccgt cgtgcatagg cacctctatc gtggtgcccg gctcgtaatc
     2581 aaccgaaatc ggctcgaacg ccggtacgaa agtgatgtcg tgcagccgct cctcgtgggc
     2641 cttgccgtac gggtagcttt tggtcgattc ttcgtggttg ttgaacgtca cgcaggggct
     2701 gatgatgtcg agcaccgccg tgccgttgta cgtgatggcg gccttgagca gctcgcggac
     2761 ctgcttggca tcgcccgcga acgaccgcgc cacgaagccg cagtccagca caatcgcttc
     2821 caggcacagg tcgattggcg gcaattcgtt ggtgcccgcg tacttgagct gctggcccat
     2881 gtccgccgtg gccgagaact ggcccttggt caaaccgtac acgccgttgt tctcgatgat
     2941 gtagaccatc ggcacgttgc ggcgtagcag gtgcttgtac tgccccagcc caatagagcc
     3001 ggtgtcgccg tcgccgctca ccgcgagggg gaggaggctg ttgttggcga gcgaagcccc
     3061 cgtgaccacc gacggcatgc ggccgtgcag ggcgttgaag ccgtgcgagc ggttcaggaa
     3121 gtacgcgggc gacttgctgg agcagccgat gccgctcatc ttaatcactt cgtaaggctt
     3181 cagcgaaagc tcgaaggcca cgttgatgat ctggctcgaa atcgagtcgt gcccgcagcc
     3241 ctggcagagg gtggagggca ggcccttgta gtcgcctttc tccagcccta taaggttgac
     3301 tttctcgttg cgttcgggtc tggctgtagc taccatgtta cttctgctcc atttccagga
     3361 ttgctttgcg aacgtaagtc gcggtcaaag gcaggccgtc gaggtgggcc accgaacgga
     3421 agtccatcga ccgctccggt atttcaagcc gcaacagcga acgcaattgc gcttcagcat
     3481 tgagctcaac tacgtagata cgcttgtgct tgctcacgaa gtcgcgcaag gtatcctcga
     3541 aggggagggc gcgcacctgc aagtagctcg tttcgaggcc ctgcttgcgc atctggtcac
     3601 gggcctcctg tatggcgggg tcggtcgagc cgtaggcgat gatgcctatc tcggcacctt
     3661 cgcggatatc gatgcggggc ttgggcatgt actgtcgggc cgtgtcgtgc ttgcgctcca
     3721 ggcgcagcag gttgcgctcc cagtcgtcgg gccgctcgct gtagaccgcg cgttcgttgt
     3781 gcccggtgcc acgggtgaaa taggccgaag cggggtggcg gttgccgggc agcgtgcggt
     3841 acggaatggc gtcaccgtcc acgtcggcat agcgcgccca actctccacc tgggccagtt
     3901 gttcctcgtt gagcaccttg ccccggtcca tcggctggtc ggggtactcg aaaggctcgg
     3961 tcatccagat attcatgccg aggtcgaggt cgctcagcac gaacaccggg gtctgcaacc
     4021 gttccgcgat gtcgaaggcg cgccagccga actcgaagca gtcgctcacc gagccgggga
     4081 gcagcaccac ctggcgggtg tcgccgtgtc cgaggaaata ggtcgaaagg aggtcggcct
     4141 gggaggtgcg ggtcgggagg cccgtgctag gccccacgcg ctgaatgtcc cagatcacgc
     4201 cggggatttc ggcgaagtag ccgagacccg cgaactcggt catcagggag atgccggggc
     4261 cggaggtgga ggtcatcgcc ctcgcgcccg cccagcccgc gcccagcacc atgccgatgg
     4321 cggcaagctc gtcctcggcc tggatgatgg cgtaggtctc gctgccgtcc tcggcgggcg
     4381 gccgcaactt gccgatgtag gccgcgagcg cgtcaaccag gctggtggag ggggtaatcg
     4441 ggtaccagga gaccaccgac acaccgccga agatggcccc caggccgccc gcagtgttgc
     4501 cgtcgatcat gatgaggccc tggttgccgt tcatgcgctc catgcgatag gggtcgcgct
     4561 tcggcaggtt ctcgttagcc cattccaggg taaggcggac gacgttcatg ttcatatcga
     4621 ccgctttggc cttgcccttg aagtggtggt tcaggccctc ctcgatctcg gcaaggtcga
     4681 tgccgagcag gtgcaccagg ccgcccacgt acaccatgtt ggccacgtgg tcgcgcagct
     4741 tggggtctat cgtggcctgt ttcatcaact ccttgacggg taagggatag ctcaccacgt
     4801 catcgcgcag gttgaggaac ttcaactcct cgttgtgcag cacgatgcca cccggcggga
     4861 gggctgcgta gtcctcctgt gcggtagccg ggttgaaggc caccagcacc tcggcgctct
     4921 cgcggcgggc tgtgtagccg tccttgctca ggcggatggt gtaccaggtg ggcaggccgg
     4981 agatattgga ggggaaaagg ttcttggcgc tgaccggtat gcccattttg aaggcggcgc
     5041 gcgccagcag gccgttggcg gtctggctgc cggagccgtt caccgtggcg actactatcg
     5101 agaagtcgtt tactatctcg tctggcctat cttgcgcctg cgatgcaggc tgctctagca
     5161 cgtccaacgt gcccataaaa tcatctccct ctattggaat tggctctaaa cgtggttgtt
     5221 acttgctctt agcctggcgt aatttcgtcg cgggggtcga ttagctcctc gcctatttcg
     5281 gtggtgccat cctcgttgcc ctgggcgttc tggttgcgct ccccgagcag gtcggcgggg
     5341 ccggacatcg gcgggattat cggcacgacc tcggtatcca ccgctcccag gtgcatgccc
     5401 ccgcccgtcg gcccgccggc ttcaggctgg cggtactcac ctacgtgggt aacaaggtcc
     5461 tggtccagtt cttccctgtc gtactcaggt tgcgcttcgg gagactgggg tgtctcctcc
     5521 tggtgttggc cggtcatcgt attctcctaa catggagtgc gcgcactcga cctatttttc
     5581 ggggccgtca aatgaggatt agtacgtaca agtatagcac atcggcacga ggcatgccat
     5641 agacgaacga gggacaggca gccctgagcc gctatctgtc cctcatcctt ctctgctatc
     5701 gtctagatgt cttcgaggtc gatc
//
LOCUS       DASRWZ010000465         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_792322, whole genome shotgun sequence.
ACCESSION   DASRWZ010000465 DASRWZ010000000
VERSION     DASRWZ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792322"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1948
                     /locus_tag="VFH60_07285"
     CDS             <1..1948
                     /locus_tag="VFH60_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET6313623.1"
                     /translation="AGVSGECVSPSAYRTCILVVDGLAMSIDTTPRTSERNSVLPTAS
                     EAVQGHVVLCGLDGLGLRTLEELHQLGEDVVVIAHTPSETFALQAHQLGATIITGNQW
                     EEPVLRQAGVTAARAIILTDKDDVANLHAGLVAQDLNPGIPVILHMFNMELGQQVKAL
                     FHNCAVLSSSEIAAPAFIAAALHPYWEQRLEVQGHTLVVRAAKAQARVWGGNKGAVKV
                     TSDPRVLLPLACIKGDGTATLFPGNETSPNAKMPAVGEVCEDVVFLVDEGPTAPSAQP
                     VEQERRQRFDLGETLRTGRRLFLAADPRLRGLSGILLVLVVISALIFSVFQGLSPLDS
                     IYFTISTITTTGFGDISLVNSHPALKVYGTLLMVLGAAALAIFYALITDVLVGARLAQ
                     VTGEDLSRRRDHVVVAGLGTIGFIVAQKLSQIGVPVVAIELNPNGRFLPGLRAIGVPV
                     VVADGRLPETLEAVGIRNARCLVTAIDGDIANLQTALTARSLNPNLRVVLRLFDPDLS
                     TRMEHTFQIHVSRSPAALAAPAMLAAAVGGQVIAPVQVADQVVFILRTTVEPGSTADG
                     NTIDCLEESAEDEEGGMEGRVLLLVENGRHTWRPPKGAILRAGHELVLAVSQRGLAHI
                     LSLTNPGPLADSQHSTASARTDGR"
     gene            2024..2926
                     /locus_tag="VFH60_07290"
     CDS             2024..2926
                     /locus_tag="VFH60_07290"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6313624.1"
                     /translation="MTLPQRPLGKTGVQVPIIGYGTAPLGKIEGPDEPSKAKAAKLLN
                     LAIDLGITYLDTSPDYGSQPKLGEVMKTRRNEVFLATKINKRKHKEVLEELQHNLKEL
                     QTDHVDLVQIHAVNVMADLEAALAPDGAIAALEEARRSGMTRFVGITGHARPSVLAHA
                     LERYDFDTVLVALGAVDRLVSGPEEALLPIARRKDVGVIAMKVYGHGELKKRELALRY
                     SLGLPGVSTAILGMKEESEIEENVRIAQAFEPLEEDELQALLEEARKLVENDKPADDS
                     PIFWIYDSKSMAWQEDSVPALVAY"
BASE COUNT          588 a          938 c          984 g          501 t
ORIGIN      
        1 cgcgggcgtc agcggggagt gcgtaagccc ctcagcatac cgcacctgta ttctagtagt
       61 tgatggttta gctatgtcga tagacaccac acccaggaca tcagaacgga acagcgtgct
      121 tcctacggca agcgaggcgg tacaagggca cgtagtgttg tgcggcctcg acgggctggg
      181 gctgcgcacg ctcgaggaat tgcaccagct cggagaggac gtggttgtga tagctcacac
      241 accctccgag acgttcgcgc tacaggccca ccagctgggt gccactatca tcaccggcaa
      301 ccagtgggag gagccggtgt tgcgccaggc aggtgtgacc gcggcccgcg ctatcatact
      361 gaccgacaag gacgacgtgg cgaacctgca cgccgggctg gtggcgcagg acctcaaccc
      421 cggcatcccg gtcatcctgc acatgttcaa catggaactg ggacagcaag tgaaggccct
      481 gttccacaac tgcgcagtcc ttagctcgtc agagatcgcc gcgcccgctt tcatcgccgc
      541 cgccctgcac ccatactggg agcaacggct tgaagtgcag gggcataccc ttgtggtgcg
      601 ggcggccaaa gcccaggcgc gagtgtgggg cgggaacaag ggcgcggtca aggtcaccag
      661 cgacccgcga gtcttacttc ccctggcgtg catcaaggga gatggcaccg ctaccctctt
      721 ccccggtaac gagacttcgc caaacgctaa aatgcccgca gtaggcgagg tgtgcgaaga
      781 cgtggtgttc ctggtggatg aaggtcctac cgcccctagc gcgcagccgg tggagcaaga
      841 gcgacggcaa aggttcgatt taggcgagac gttgcgcacg ggccggagac tgttcctggc
      901 agcggaccca cgcttgcgcg ggctgtcggg gattctcctc gtgctggtgg tcatcagcgc
      961 gcttatcttc tcggtgttcc agggcctcag ccctctcgac tcgatctatt tcacaattag
     1021 caccatcacc actaccggct tcggagatat ctccctcgtc aactcccacc ccgcgctgaa
     1081 agtgtacggc acgctcctca tggtgcttgg tgcggcggcc ctggcgatat tctacgcgct
     1141 cataaccgac gtgctggtgg gcgctcgcct cgcgcaggta acgggcgagg acctcagccg
     1201 ccggcgggac cacgtggtag tggcgggcct gggcacgatt ggtttcatag tggctcagaa
     1261 gctctcccag attggcgtac ccgtggtcgc gattgagctg aaccccaacg ggcgtttcct
     1321 gccgggcctg cgcgccatcg gtgtgccggt ggtcgtggcc gatggcaggc tgccggagac
     1381 gctcgaagcg gttggtatca ggaacgcccg ctgcctggtc acagcgatag acggcgacat
     1441 cgcgaacctg caaaccgccc tcaccgcgcg ctcgctcaac cccaacctgc gcgtcgtgct
     1501 gcgcctgttc gaccccgacc tatcgacgcg gatggagcac accttccaga tccacgtgtc
     1561 gcgcagccct gcggcgctgg cagcccccgc catgcttgcg gcagccgtcg gcgggcaggt
     1621 aatagccccc gtgcaggtag ccgaccaggt ggtattcatc ctgcgaacta cggttgagcc
     1681 tgggtccacg gcggatggca acacgattga ctgcctggag gagagcgcgg aggacgaaga
     1741 agggggcatg gaggggcgcg tgctcctgct ggtggaaaac ggcagacaca cttggcggcc
     1801 cccgaagggc gctatcctgc gcgccgggca cgaactggtc ctggcggtgt cgcagagggg
     1861 cctggcccat atcctgtcgc tgaccaaccc cggaccgctc gccgacagcc agcactccac
     1921 ggcctcggct cgcacggacg ggcgctagtt gccgccggag ggtacgtaag ttgcaatagg
     1981 tcatgctccc aatactgagc ttcacacgtt aggagagaac accatgacgc taccacaacg
     2041 acctctcggc aagacgggcg tacaggtgcc catcatcggc tacggcacgg cccccctcgg
     2101 caagatagag gggcctgacg agccatcgaa ggccaaggcc gccaagctgc tcaacctggc
     2161 tatcgacctg gggataactt acctcgatac cagccccgac tacggcagcc agcccaagct
     2221 gggcgaggtg atgaagaccc gccgcaacga ggtgttcctg gctaccaaga tcaacaagcg
     2281 caagcacaag gaagtgcttg aggagttaca gcacaatcta aaggagctac agaccgacca
     2341 cgttgacctg gtgcagatac acgccgtcaa cgtcatggcg gacctggaag cggccctcgc
     2401 gcccgatggg gccattgccg cgctggaaga ggcccgccgc agtggcatga cccgcttcgt
     2461 gggcattacg gggcacgcgc ggccctctgt gctggcgcac gcgctggaga ggtacgactt
     2521 cgacacggtg ctggtggccc tgggcgcggt ggaccgcctc gtgagcggcc ctgaggaggc
     2581 gctgctgccc atcgcgaggc gcaaggacgt gggcgtgata gcgatgaagg tgtacgggca
     2641 cggcgagcta aagaagcggg agctggcctt gcgctactcg ctggggctgc ccggcgtgag
     2701 caccgccatc ctgggcatga aggaggagag cgagatagag gagaacgtcc gcatcgccca
     2761 agccttcgag cccctggaag aagacgagtt gcaggccctg cttgaggagg cgcgcaagct
     2821 ggtggagaac gacaagcccg ccgacgacag ccccatcttc tggatttacg acagcaagtc
     2881 gatggcctgg caggaggata gcgtaccggc gctggtggct tattaggcgt tgatgaaggg
     2941 caatgttgcc ctgggatagc cctgtggctg aaaccacggt cttacaagga acaaggtctc
     3001 cttcggaaac t
//
LOCUS       DASRWZ010000466         5295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1066423, whole genome shotgun sequence.
ACCESSION   DASRWZ010000466 DASRWZ010000000
VERSION     DASRWZ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066423"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VFH60_07295"
     CDS             <1..1066
                     /locus_tag="VFH60_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HET6313625.1"
                     /translation="DIGRDPPALLAPADEVRFVPTDELSFDAASGRRRDEPQEPGTAP
                     VFAVEEPGALTLLQDLGRPGFAHLGLSAGGAMDPDALTLANHLVDNSRDAACLEITWS
                     GPVLRALATTVIAVVGGELGCSVDGSRVPNGVSWLVRGGATVRFTGPGRGNGDARCHL
                     AVAGGFSAAPALGSRSTYLPGSFGGYHGRQLQRGDILHTYRHARPAAELAGRSEPISA
                     SRQHGTVLLRYVPFEGEGSVPVRVRGLALGQEYEVSPASDRMGVRLLPEQEPLLQAGG
                     GELASFGVVRGAIQVPPGGTPVVLGAAHQTTGGYPLLGVVARADWPALAQLKPGRKVR
                     LQPIALAEARAARLAGSTRR"
     gene            complement(1080..1349)
                     /locus_tag="VFH60_07300"
     CDS             complement(1080..1349)
                     /locus_tag="VFH60_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313626.1"
                     /translation="MADSYYPTRQDPGMSGQNVVPGPSGEDIDANPGERALTPAGSAT
                     DATVSGVTVGNKQIGVGAQVGILRNLEFTAGGRAHRVSTGPYTKA"
     gene            complement(1359..3017)
                     /locus_tag="VFH60_07305"
     CDS             complement(1359..3017)
                     /locus_tag="VFH60_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET6313627.1"
                     /translation="MITSGDRTKGEDKVLKPALERSPERQPEFRTASNLPVKRIYTPD
                     DAPRGGYEEELGYPGQFPFTRGVQATMYRGRFWTMRQYAGYGTAEESNRRYKYLLAQG
                     QTGLSVAFDLPTQMGYDADHPLAAGEVGKVGVAISSLRDMELLLADIPLDKVSTSMTI
                     NATASILLAMYVAVARNRGISDDVLTGTVQNDVLKEYIARGTYIYPAAPSMRLAGDII
                     AFCAEQLPQWNPISISGYHIREAGSTAVQEVAFTLSNAVAYVGEVLGRGISVDKFAPR
                     LAFFFIAHSDFLEEIAKYRAARRLWARIMRDELGAQSPQSMMLRFHTQTAGSSLTAQQ
                     PDNNVIRVALQALSAVFGGTQSLHTNSLDEALALPTERSARIALRTQQIIAHETGVAS
                     TIDPLAGSYYLEWLTGQIEEGARDYLRRIDAMGGTLHAIEEGFQQQEIQEASYRWQTE
                     VDSGERVIVGVNRFRSEDVEATDIHRVNPELQARQVERLHALREERNDADVAAGLERL
                     RNAAGDRDNLMPFIIDCVQSLSTLGEISDALRAVFGLYEERRLV"
     gene            complement(3048..3569)
                     /locus_tag="VFH60_07310"
                     /pseudo
     CDS             complement(3048..3569)
                     /locus_tag="VFH60_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
     gene            complement(3573..5099)
                     /gene="accC"
                     /locus_tag="VFH60_07315"
     CDS             complement(3573..5099)
                     /gene="accC"
                     /locus_tag="VFH60_07315"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET6313628.1"
                     /translation="MRKLKKVLIANRGEIALRVVRACAEVGLESVAVYSEADRTSLHV
                     RAADEAYLIGPPQSTDSYLRVDRLLDVARKSGADAVHPGYGFLAENADFAQAVLDAGL
                     TWIGPEPRAMRMLGDKLEARRTAQAAGVPTVPGTDVATDDLQTALEAAQRIGYPVLIK
                     ASAGGGGKGMRIVSRPEELEAALRGAQSEARASFGSGTVYIEKYLESVRHVEIQLLGD
                     THGNVIHLGERECSVQRRYQKLIEESPSVAVDRDLRERMGSVAVAAAKAVGYSSAGTV
                     EFLLDKFKNFYFLEVNTRLQVEHPVTELVTGVDLVLEQFRIASGRPLRLKQSDVRFNG
                     HAMECRIAAEDPFNNFAPSLGTIAAVSEPSGAGVRVDSSVYAGGKVSIYYDPMIAKLI
                     VWAPSRAHAILRMRRALREYRIAGIQTNIPFHLRVMQSLDFQRGRLDTKFVERLLGDG
                     ARSVQSTDDDLEHIAAMSAALVLDRKRQAGAASAAGPAQNPSADGNAWRRAGRRQGLR
                     "
BASE COUNT          860 a         1714 c         1725 g          996 t
ORIGIN      
        1 ggacattggc cgagacccac ccgcgctgct ggccccggcc gatgaggtgc gcttcgttcc
       61 tacggacgaa ctgtcgttcg acgcagcaag cgggcggcgt agggacgaac cgcaggagcc
      121 tggcactgca cctgtttttg ctgtcgagga gccgggggcg ctcactctgc tgcaagacct
      181 gggcaggccg ggattcgctc acctcggcct gagcgcgggt ggggcgatgg accctgacgc
      241 gctgactctg gccaaccacc ttgtagacaa ttcgcgtgat gctgcctgcc tcgaaatcac
      301 atggtccggg cctgtgctga gggcgctggc aacgacggtc atcgcagtag tcgggggcga
      361 acttgggtgt agcgtggatg gaagccgcgt acccaacggt gttagctggc tggtgcgcgg
      421 gggtgctacg gtgcggttca ccgggccggg tcgcggcaac ggggatgctc ggtgtcatct
      481 ggcggtggcc gggggcttca gcgccgcacc tgcgcttggt tccaggtcta cctacctgcc
      541 cggaagcttc ggcggctacc atgggaggca gttgcaaagg ggcgacatcc tgcacactta
      601 ccgccacgcc aggccggccg ctgagcttgc ggggcggtcg gaacccatat cggcaagtcg
      661 gcagcatggc acggtcttgc tgcgctacgt accttttgag ggcgagggga gcgttccggt
      721 cagagttaga ggtcttgccc tggggcagga atacgaagtc agcccagcga gcgacaggat
      781 gggcgtgcgg ttgctgccgg agcaagaacc gttgttacag gctgggggtg gagagcttgc
      841 gtcgtttggt gtggtacgcg gcgcaatcca ggtgccaccg ggaggcacgc cggtggtgct
      901 gggggcagcc caccagacca cgggcggcta tccactgctg ggggtggtcg cacgagcaga
      961 ctggcccgcc ctggcgcaac taaaaccggg gcgcaaagtc aggctgcaac ccatcgcgct
     1021 cgcggaagca agggctgctc ggctggccgg ttcaaccagg cggtgacccg gagagggcgc
     1081 taagccttcg tatagggtcc ggtgctcacg cgatgggcgc ggccccctgc ggtgaattcc
     1141 aggttgcgca ggatgccgac ctgcgcgccg accccgatct gcttgttccc gactgtgaca
     1201 cccgacacgg tggcgtccgt ggcgctgccc gcgggtgtaa gcgcccgctc tccagggttg
     1261 gcgtcgatgt cttcgccgga gggaccgggc actacgtttt gtcccgacat tccggggtct
     1321 tgccgtgtgg ggtaataaga atctgccatg ctgctctcct aaactagccg ccgttcctcg
     1381 tagagcccga acacggcccg cagcgcgtcg cttatctcgc ccagcgttga gaggctctgc
     1441 acgcagtcga taatgaacgg catcaggttg tcgcggtctc cggcggcgtt gcgcagacgc
     1501 tccaacccgg cggcgacgtc tgcatcgttg cgttcctctc gtagggcgtg cagacgctcg
     1561 acctggcggg cctgcaactc aggattcacg cggtggatat ccgtggcctc tacgtcttcc
     1621 gaccggaagc gattgacgcc cactatcacg cgctcgccgc tgtccacctc agtttgccag
     1681 cgataagatg cttcctgtat ctcctgctgc tggaaccctt cctcgatagc gtgcagggtg
     1741 cctcccatcg catcaatcct gcgcaggtag tctcgcgcgc cctcctcgat ctggccggtg
     1801 agccattcca ggtagtacga gcctgccagc gggtcgattg tggaagccac gccggtctca
     1861 tgcgcgatta tctgctgggt cctcagggct atgcgggcgg agcgttccgt cggcagggcc
     1921 agggcctcgt caagagagtt ggtgtgcagc gactgagtgc cgccgaacac cgccgacagc
     1981 gcctgtagcg ccaccctgat gacgttgttg tcgggctgtt gcgcggtgag gctggaaccg
     2041 gccgtctggg tgtgaaagcg cagcatcatg ctctggggac tctgcgcgcc aagctcatct
     2101 cgcataatcc gtgcccagag ccgccgcgcc gccctgtact tggcgatctc ctcaaggaag
     2161 tcgctgtgag cgatgaagaa gaacgccagg cgcggggcga acttatcgac cgagatgccc
     2221 ctgcccagca cctcgccaac gtaggccaca gcgttcgaca gggtaaaggc cacctcttga
     2281 acggccgtgg agcctgcttc gcgtatgtgg tagccgctga tcgagattgg gttccactgg
     2341 ggcaactgtt ccgcgcagaa ggcgattatg tccccggcca ggcgcatgga gggggcagca
     2401 gggtagatgt acgtgccgcg cgcgatgtat tccttcagca cgtcgttttg gacggtgccg
     2461 gtcagcacgt cgtcggagat gccgcgatta cgcgccactg caacgtacat agccaggagg
     2521 atggaggcgg tggcgttgat ggtcatggag gtgctgactt tgtcgagcgg aatgtccgcc
     2581 agcagcagct ccatgtcccg cagcgacgat atcgccacgc ccaccttgcc cacttctccg
     2641 gcggccaggg ggtggtcggc atcgtagccc atctgtgtcg gcaggtcgaa ggccacggag
     2701 aggccggtct gcccctgtgc gagcaggtac ttgtatcgcc tgttcgactc ttcggcggta
     2761 ccgtacccgg catattggcg catcgtccag aaccgcccgc ggtacatggt ggcctgcacc
     2821 ccgcgggtga aggggaactg tccggggtag ccgagttcct cctcgtagcc accccggggc
     2881 gcgtcgtcgg gggtgtagat acgcttcacc gggaggttgg aggcggtgcg gaactccggc
     2941 tggcgctccg gggaccgttc gagggcgggc ttcagcacct tgtcttcccc cttagtcctg
     3001 tcgccggaag taatcatggg tagccttacc tcgtccaggg tcacgagcgg cttgtccgct
     3061 tcgaccaccg acccggcctc gacgtgcacc tgggccactc gcccggcgcg cggcgcggtg
     3121 atttcatttt ccatcttcat ggccacgagc actacaagtc gcgtgcccac gtcaacctgc
     3181 gccccggctt ccaccagcac ttccttcacc agccccggca tcggcgcgtt caccaccatg
     3241 ctgccgccct gggccatctg cttgggtgcc accttcatga gccgccattc gcgctcggtg
     3301 tgcaccttca catcgtaccg gtgccgccag atcatcacgc ggtagtgctc gtcacgcttc
     3361 tcgacgtaga gttggtagct gcgtgcgttg gcgatcaggg aatacagccc ctccccggtc
     3421 tcgacgttgg cgtggaccgg cacgaagtcc acccccaccc cgttgctgcc ctcctcatcg
     3481 gtgaggggga ccaggtgcgt gacgccccct gcctgggaga tctcgaagcc aagctcctgg
     3541 ccgttaagct ccacaaaata cctcggcatg gcctacctca gcccctggcg gcgaccggcc
     3601 cgccgccacg cattgccgtc tgcgctcggg ttctgcgcag ggccagccgc cgacgcggcc
     3661 cccgcctgcc gcttgcggtc gagtacaagg gctgcggaca tggcggctat atgttctagg
     3721 tcgtcgtcgg tggactggac gctcctggca ccgtcgccca gcaaccgctc gacgaacttc
     3781 gtatcgaggc ggccgcgctg aaaatcgagc gactgcatta cgcgtaggtg gaaggggata
     3841 ttggtctgaa taccggcaat ccggtactcg cgcaacgccc ggcgcatcct gaggatggcg
     3901 tgcgcgcggc tcggtgccca aacgatcaac ttggcgatca tcgggtcgta atagatagac
     3961 actttgccgc ccgcgtagac gctgctatct acacgcaccc cggccccgct cggctccgat
     4021 accgcagcta tagtgccgag agacggggcg aagttgttga aggggtcttc agccgcgata
     4081 cggcactcca tcgcgtgccc gttgaaccgc acgtccgact gcttgaggcg taggggacgg
     4141 cccgaggcta tgcgaaactg ttcaagcacc aggtcaacac ccgtgacaag ctccgttact
     4201 gggtgctcca cctggaggcg cgtgttcact tcgaggaagt agaagttctt gaacttgtcc
     4261 agcagaaatt ccactgtgcc ggcagaggag taacccacag ccttcgcggc ggccacggcc
     4321 acgctgccca tgcgctcgcg taggtcccgg tcgaccgcca ccgacgggga ctcctcaatc
     4381 agcttctggt agcggcgctg gaccgagcat tcgcgctcgc ccaggtggat gacgttgccg
     4441 tgagtgtctc caagcagctg tatctcgacg tgccgcaccg actccaggta cttctcgatg
     4501 taaacggtgc ccgaaccgaa cgatgcgcgg gcttcactct gagcgcccct gagcgcggcc
     4561 tccagctcct ccggccggga cactatgcgc atgcccttcc cgcccccacc agccgaggct
     4621 ttgatgagca cgggataacc gatccgttga gccgcctcaa gggccgtctg taggtcgtcg
     4681 gtggcgacgt ccgtgccagg gaccgtgggc acgcccgccg cctgcgcggt cctgcgcgcc
     4741 tctagcttgt cgcccagcat gcgcatggcc ctcggctcgg ggccaatcca ggtgagtccc
     4801 gcgtcgagta cggcctgcgc gaaatcggcg ttctcagcca gaaagccata gccggggtgc
     4861 accgcatcag cccccgactt gcgcgctacg tccagcagcc tgtctacgcg caggtaggag
     4921 tcggtggact gcggcggtcc gatgagataa gcttcgtcgg cggcgcggac gtgcagcgag
     4981 gtgcggtcgg cctccgagta gacggcgacg gactccagtc ccacctcggc gcaggcccta
     5041 accacgcgca gggcgatctc ccccctgttg gctatcaaga cttttttcag cttacgcaat
     5101 cccgtgctcc atccggttct gttacgtttg ttcgaatcag gccgcgcact gtgccaggta
     5161 tgcctggagg tagcggctgg cgggaggctt gttcaacacg ccggtgatga aacgcgacgc
     5221 ttccaccact ttttcaagct gcacgccggt ctctatgccc agcccgtgga gcatatacag
     5281 caggtcctcg gtcgc
//
LOCUS       DASRWZ010000467         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_243727, whole genome shotgun sequence.
ACCESSION   DASRWZ010000467 DASRWZ010000000
VERSION     DASRWZ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243727"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VFH60_07320"
     CDS             complement(<1..1042)
                     /locus_tag="VFH60_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HET6313629.1"
                     /translation="MKKRHSRALATRRPAPAPRGAQTPFLHRKSSGQAMVIIVLSLFV
                     LMAVVGLAIDGGSNYQQRRKAQNGADAAALAGARKMLALYEDMVESYDSDVNGSASKE
                     DLIRTEIENIATANGIVTTTTDLVAYFVNAQKQVVTATSGDGCGTASPCEVGKNNMVP
                     WKMGARGIYVTTRSETAAFFMPVIGYNTVSATASATAFMGVAINTQQNIGLMPIGFFT
                     ETERLAQLQPGRTYTLISGSTKRGSGNWGYVDFNGDGNPAPVVDAWIACGFNPSLSTN
                     AQWAEWCASKGYGDSEHASEYRAKGKTQYWTGTAEPLKGPYYEPRFDYMAGWWLAGSS
                     GSTNSTCQVFQEL"
     gene            complement(1071..>1624)
                     /locus_tag="VFH60_07325"
     CDS             complement(1071..>1624)
                     /locus_tag="VFH60_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HET6313630.1"
                     /translation="RIQPPKRQTPTQGRARTKTKGQALVEFALMGLFLGALLAGAIDF
                     GRAYYTSIIVTNMAGEGAAYASIYPDRDKDPADPTNEQCILTRTVATHQSIQERARKV
                     AQERGLIVEKQDQSQARIEVFTEGYGSTCSARCPGRTITVRITYTLDDLFLPNMLGLK
                     EIPITKSASQVITGEPFYSDCPN"
BASE COUNT          226 a          516 c          524 g          358 t
ORIGIN      
        1 ctagctcctg gaacacctgg caggtgctgt tcgtgctgcc gctcgacccc gctagccacc
       61 agcccgccat gtagtcgaag cgcggctcgt aatatggccc cttcagcggc tcggcggtcc
      121 cggtccagta ctgggtcttg cccttggcgc ggtactcgct ggcgtgctcc gagtcgccgt
      181 agcccttgct cgcgcaccac tcggcccact gtgcgttggt gctgagcgag gggttgaagc
      241 cacaggcgat ccaggcgtcc accaccgggg cggggttacc atccccgttg aagtccacgt
      301 agccccagtt gcccgagcct cgcttggtgc tgccgctgat gagcgtataa gtccgtccgg
      361 gttggagctg ggccaaccgc tccgtttccg tgaagaagcc gatgggcatg aggccgatgt
      421 tctgctgggt gttgatggcg acccccatga aagcggtggc cgaggcggtg gcgcttaccg
      481 tgttgtagcc tatcaccggc atgaagaaag cggccgtctc cgagcgggtg gttacgtaga
      541 tgcccctggc ccccatcttc cacggcacca tgttgttctt gcccacctcg cacgggctgg
      601 cggtgccaca cccgtcgccc gatgtggcgg taaccacctg cttctgtgcg ttgacgaaat
      661 aggccaccag gtcggtcgtc gtggtgacga tgccgttagc ggtggcgatg ttctctatct
      721 cggtcctgat aaggtcttcc ttgcttgccg agccgttcac gtcgctgtcg tagctctcga
      781 ccatatcttc gtacagggcg agcatcttgc gcgcccccgc cagggccgcc gcgtccgccc
      841 cgttctgggc cttgcgccgc tgctggtagt tggagccgcc atctatcgca agccccacga
      901 ccgccatcag cacgaacagg ctgagcacga tgatgaccat cgcctggccg ctgctcttgc
      961 gatggaggaa cggcgtttgc gcgccccgcg gtgcgggtgc cggtctccgt gtagccagcg
     1021 ctctgctatg tcttttcttc atcgtgccgt ccttatatct gtgtttctca ttagttaggg
     1081 cagtcgctgt agaacggctc gccggtgatt acctgcgagg ccgactttgt aatgggtatc
     1141 tccttgaggc cgagcatatt gggcaggaac aggtcgtcca gcgtgtaggt gatgcgcacc
     1201 gtgatcgtcc tgccggggca gcgggccgag caagtggacc cgtagccctc ggtgaacacc
     1261 tcgatgcggg cctggctctg gtcctgcttt tcgacgatga ggccgcgctc ctgggcgacc
     1321 ttgcgggcgc gttcctgtat gctctggtgg gtggctaccg tcctggtgag gatgcactgc
     1381 tcgttggtgg ggtcggcggg gtctttgtcg cggtcggggt agatggaagc gtaggccgcg
     1441 ccctcgcccg ccatgttggt cacgatgatc gacgtgtagt aggcgcgccc gaaatcgata
     1501 gcacccgcca gcagcgcccc caggaacaag cccatgagcg cgaactccac cagcgcctgg
     1561 cccttcgtct tcgtcctggc cctgccctgt gtgggggtct gcctcttcgg cggttgtatt
     1621 cgtt
//
LOCUS       DASRWZ010000468         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_243929, whole genome shotgun sequence.
ACCESSION   DASRWZ010000468 DASRWZ010000000
VERSION     DASRWZ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243929"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1967
                     /locus_tag="VFH60_07330"
     CDS             <1..1967
                     /locus_tag="VFH60_07330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313631.1"
                     /translation="ANRKRSDEELFARFGQPGRVHLLNHATDAPYSAAHHALLHEFGI
                     ERLWDGQPKEVLLISPDVLPVGDIPASGSGIRAWALGKGLESRGHKVRFTMPRAALQG
                     REGQVPVEYARGAWTAENLQSMVDALAPDVIVSCGWPNLTWLLRANLPIALDLTGPHL
                     LERIYQEHLDARANSEEKLAALQRADFFTCIGERQRHYFTGWLAQAGLHPDEIGTSLQ
                     VIPYSLDPDQPPHVWPSEWEGEEVLFVYGGIFLPWQNPAPALLTVATALQEQGRGRLE
                     VIGGKHPFHAVHTGGFGPLVDRLATMPSVQMSGLLPHDALVERYTRAHVAVDVMQPNA
                     ERLLAFPSRTVHYLWCGLPVIHAAFSEVAAVIREYEAGWVVRHDDPDGLRAIVNSILA
                     DPAEAQRRGENAARLARERFTWDITVDALDRFVREPYIRSSRMSRATSQHASSAPADP
                     ARNVTVSGSAPLAVGQNAGRPDFARKLQATHDRRRAAPAQVRARARELLRGVKGAVGA
                     PLGVGVQLPELVTGHSLGQRFYVPENGLSGVSVQVETFGRRNTSRLFLRLRDNPGAGG
                     DIYHIEIPAHDLSQAQVLTLRFPPLRDSAGRSFYFLAESPDGVPGDAISLRGTTQAGN
                     LRAQRYDDGIPASGYLVMSLEYNGVAGGAR"
     gene            2021..>2140
                     /locus_tag="VFH60_07335"
     CDS             2021..>2140
                     /locus_tag="VFH60_07335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET6313632.1"
                     /translation="MVIPTYNNLGLLRDCLRSMHALDYSGGKLEVTVVDNGSSD"
BASE COUNT          369 a          728 c          706 g          337 t
ORIGIN      
        1 aggccaaccg caagaggtct gacgaggagc ttttcgcccg cttcggccag ccggggcgcg
       61 tgcacctgct caaccacgcg accgatgcgc cctactcggc ggcgcaccac gcgctgctac
      121 atgagtttgg catagagcgg ctgtgggacg ggcagccgaa agaggtgctg cttatcagcc
      181 ccgacgtgct gcccgtgggc gacataccgg ccagcggctc aggcatcagg gcgtgggcgc
      241 tcggcaaggg gcttgaaagt cgcgggcaca aggtgcgctt caccatgccg agggccgcgt
      301 tgcagggtag agaggggcag gtgccggtgg agtacgctcg tggtgcttgg acggccgaga
      361 acctgcaaag tatggtggat gcgctcgccc ccgacgtgat cgtgtcctgc ggctggccta
      421 acctgacctg gctgctccgc gccaacctgc ccatcgcgct ggacctcacc gggcctcacc
      481 tgctcgaacg catctaccag gagcacctcg acgccagggc caactcggag gagaaactgg
      541 cagcattaca gcgagccgac ttcttcacct gcatcggcga gcgccaacga cactatttca
      601 ctggctggct ggcgcaggcg ggcctccacc cggacgaaat cggcacgtcc ctgcaggtca
      661 tcccgtactc cctcgatccc gaccagccgc cgcacgtctg gccgtccgag tgggagggtg
      721 aggaggtgct cttcgtgtac gggggcatct tcctgccctg gcagaacccc gcgcccgcct
      781 tgctgaccgt cgccaccgcc ttgcaggaac agggacgcgg gcggcttgaa gtcatcggcg
      841 gcaagcaccc cttccacgcg gtgcatacag ggggattcgg ccccctagta gaccggttgg
      901 ctacgatgcc gagcgtgcaa atgtcgggcc tgctgccgca cgacgcgctc gtggagcggt
      961 acaccagggc gcacgtggcc gtcgatgtaa tgcagcctaa cgccgagcgc ctccttgcct
     1021 tccccagccg gaccgtgcac tacctgtggt gcggcctccc cgtgatacat gccgccttta
     1081 gcgaggtggc tgccgtcata cgcgagtacg aggcgggctg ggtggtgcgg cacgacgacc
     1141 ccgacggcct gagggcaatc gtgaacagca tcctggccga ccccgccgaa gcccaacgcc
     1201 ggggcgagaa cgccgcacgc ctggcgagag agcggttcac ctgggacatc accgtggacg
     1261 cgctggaccg cttcgtgcgc gaaccataca tccgaagctc ccgcatgagc cgcgctacct
     1321 cccagcatgc ctcgtccgct cctgccgacc ccgcgcgaaa cgtgacagtc agcggcagtg
     1381 ctcccctggc ggtggggcag aacgccgggc gacccgattt cgcccgcaag ctccaggcga
     1441 cacatgaccg gcggcgcgcg gcacccgcac aggtgagggc gagggccagg gagcttttga
     1501 ggggcgtaaa gggggcagtc ggcgcaccgc tgggcgtggg cgtacagctt cctgagcttg
     1561 tcaccgggca cagcctcggc cagagatttt acgtcccgga aaacggcctt tcgggtgtgt
     1621 ccgtgcaggt ggagacgttt ggcaggcgca acacctcgcg cctcttcctg cgcctgcgcg
     1681 acaacccagg ggccgggggc gacatatacc acatagagat accggcgcat gacctgagcc
     1741 aggcccaggt gctcacactc cggttcccgc cccttcgtga ctcggccggt cgctccttct
     1801 acttccttgc cgagtcgccc gacggggtgc caggtgacgc cataagcctg cgcggcacga
     1861 cgcaagccgg caacctgcgc gcccagcgtt acgacgacgg catccccgcg tccggttacc
     1921 ttgtaatgag ccttgagtac aacggagtgg cgggaggtgc caggtgaacg ccactcgtcc
     1981 tgacgctaag ggcccgcaac cgcatgcgcg gagcgtgtcg gtggtcattc caacttacaa
     2041 caacctcggc cttctgcgcg actgcctgcg gtcgatgcac gcgctcgact actcgggggg
     2101 caagctcgaa gtgacggtag tggacaacgg ttccagcgac
//
LOCUS       DASRWZ010000469         4090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_450028, whole genome shotgun sequence.
ACCESSION   DASRWZ010000469 DASRWZ010000000
VERSION     DASRWZ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450028"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1730
                     /locus_tag="VFH60_07340"
     CDS             96..1730
                     /locus_tag="VFH60_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET6313633.1"
                     /translation="MSLLVTAAEMRAIEAEAVSRGATWQGLMARAGAQVARLSTLWLG
                     PETQQRILVLAGPGNNGGDALVVARHLADHGWHVRLRTWHRAGGRDEWLVSPLMARNI
                     GAQVLDEQAALSADLEWATAIIDGLLGTGLQRDVDAELASIINQIAASGRKVIAIDIP
                     TGVDSDTGQVRGAALRADCTVALGHLKYGHVIQPGARLSGKITLGEIGLSVKTSRETA
                     SGELLTDDLVREMLPARPDDANKGTFGKAMVVAGSVNYIGAAALATEGAMRAGAGLVT
                     LGCPGDLLSVLAVKLTECTFIPLPSDMGALSLHAAEKVRSSIEGYDALLIGCGLGKEK
                     QTANFLRSLLSHTDVAARPSARPIGFSARVAEKEAQEELEGNLPPLVLDGDALNLLAE
                     WDDWSSNLPEGTVLTPHPGEMARLMDITVEDVQKDRVGVAREAASKWGALVVLKGAGT
                     VIAQPDGKVYVSPFSNPALATAGTGDVLAGAIVGLIAQGASPTNAACAGVYLHGMAGE
                     LLRERYGVAGGLAGELPRLMARAQRRLRESVDAERA"
     gene            1756..2979
                     /gene="alr"
                     /locus_tag="VFH60_07345"
                     /pseudo
     CDS             1756..2979
                     /gene="alr"
                     /locus_tag="VFH60_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
     gene            2976..>4090
                     /locus_tag="VFH60_07350"
     CDS             2976..>4090
                     /locus_tag="VFH60_07350"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET6313634.1"
                     /translation="MRAPELIAAKRDGKELTGAEIRWFVDGFTRGDIPDYQLTAWLMA
                     VYFQGMSARETAYLTLAMVESGRRLDLSVVGPFVADKHSTGGVGDKTTLVVAPLVAAA
                     GVPVGKMSGRGLGFSGGTIDKLESIKGFRAQLEPDEFVEGVRRVGLVVAAQTADLAPA
                     DGKLYALRDVTATVESIPLIASSIMSKKIAAGATGVVLDVKVGRGAFMKTIEEARELA
                     VAMRDIGTNVGLQVRAVLSGMDQPLGWAVGNALEVAEAVQTLKGEGPEDLTEVAFTLG
                     SHLLHMAGKVADVDEGVEMMRRALNSGGGLRKFRQFVENQGGDPSFVDDPLLLPQAPV
                     RRHLLAQNSGSVSSIDAEIIGRASVEIGAGRAVKGAP"
BASE COUNT          744 a         1209 c         1439 g          698 t
ORIGIN      
        1 gccgagcagg ctggccttag ccatcgggcg atcagcctca cccacagtcg ccacatggcc
       61 tgcgcgatgg tcgtagcctg caccgagaga tagacgtgtc gctcctcgtc accgcagccg
      121 agatgcgcgc tatcgaagcc gaagccgtct cgcggggtgc gacgtggcag gggttgatgg
      181 cgagggcggg cgcgcaggtg gcgcgactct ctactctgtg gcttggcccg gagacccagc
      241 agcgcatact cgtgctcgcg gggccgggca ataacggcgg ggatgcgctc gtggtggcgc
      301 gtcacctggc ggatcacggc tggcacgtgc gccttcgcac atggcacagg gccgggggca
      361 gggacgagtg gcttgtgtcg cccctcatgg cacggaatat cggggcgcaa gtcttagatg
      421 agcaggcggc gctgtctgcc gacctcgaat gggccaccgc cattatagat ggtctgttgg
      481 gcaccgggtt gcaacgcgac gttgatgcgg aattagcaag tataatcaat cagatagccg
      541 cgtcaggccg caaagtgatt gctattgaca tccccaccgg cgtagacagc gacacgggac
      601 aggtgagggg cgcggcgctc agagcagact gcacagtcgc cctggggcac ctcaaatacg
      661 gacacgttat tcagcccggc gcgagactgt cgggcaaaat cacgttagga gagataggat
      721 tgagcgtcaa gacaagccgc gagacggcat caggcgaatt acttacagac gacctcgtgc
      781 gcgagatgct gcccgcgcgc ccggacgacg cgaacaaggg cactttcggc aaagcgatgg
      841 tggtggcagg ctcggtgaac tacataggcg ctgctgccct ggcgactgag ggggcgatgc
      901 gggcgggcgc gggcctggtc acgctgggct gccccggcga cctcctttcg gtcctcgcgg
      961 tcaaactgac cgaatgcacc ttcataccat tgccttcgga catgggcgcc ctgtcgttgc
     1021 acgccgccga gaaggtgcgg tcttcgattg agggttatga tgccttgctg ataggttgcg
     1081 gcctgggcaa agagaagcag accgccaact tcctgcgcag cctcctctcc cacaccgacg
     1141 tagcagcgcg gccaagcgcg cggcccatcg ggttctcggc cagggttgcc gaaaaagagg
     1201 cacaagagga gttagaggga aatctgcccc cactggtgct ggacggcgac gccttgaacc
     1261 tgctcgccga gtgggacgat tggtcgtcca acctgccgga aggcacggtg ctgacacccc
     1321 acccagggga gatggccagg ctcatggaca tcaccgtaga agatgtgcag aaggaccggg
     1381 tgggtgtcgc cagagaagcg gcgtcaaagt ggggcgcgct cgtggtgctg aagggcgcgg
     1441 gcacggtgat cgcccagcca gacggcaagg tgtacgtcag cccattctcc aacccggcgc
     1501 tcgctacggc ggggacgggc gatgtgctgg cgggcgcgat agtgggtctc atcgcgcagg
     1561 gcgcgtctcc caccaacgcg gcgtgtgcgg gcgtatacct gcatggaatg gcaggcgagt
     1621 tgttgcgcga gagatacgga gtggccggtg ggctggcggg cgaactcccc aggttgatgg
     1681 cccgcgccca gcgtcgcctg cgggagagcg ttgatgcaga gcgtgcgtga taatccggct
     1741 gaggcacagg aggatatgga cgcggcctca tgggggcggc caatcagggc tgaaatagac
     1801 ctgtccgcaa tcgcctccaa cgtgcgcgcg ctcaggaagc taatcggccc tcgttgccgc
     1861 gtgatggctg tcgtgaaggc taacggctac ggcctgggcg cgccctgggt ggccgacgct
     1921 gcccggtagg gcggcgcttc catgctggct gtggcgtgcg tggacgaagg tgttgagcta
     1981 cggcgcgccg ggtatagcgg ccccatactg gtgatgagct acgtgccacc cgacgaagct
     2041 gacgctgcgg tgcgtaatgg gctgaccctg gtgctccacc gcttgcgcac ggcggtggcg
     2101 ttggacgaag ccgcccgcaa gcagggtttg cggcacggga ccgtagccgt tcacataaag
     2161 gtggacacgg ggctgggccg gtacgggtgc ctgcccggcg agttcctgcc actggcgcag
     2221 ggcatcatga ggttggagca cctacgcctg gaagggctga tgacccattt cgccaatgcc
     2281 gattctcagg acctcagctt cacccgcgag caactgtccc gcttcaattg ggtgcagagg
     2341 gaggcagagg agcatggcat ccaatttgac ataatacacg ccgcgaacac ggctgccgcg
     2401 ctcgcgcttc ctgagtcgcg cttcgacatg gtgcgcgccg gtatattgct gagcggccat
     2461 cttcctgctc cacatttaca gggcaccgtc cggttggagc ctaccctcac cctgcgcagc
     2521 cggttggccc gtgtctttcg ggttgaggaa ggcgcgacta taggctatgg gcggatctgg
     2581 ctggcagaac gtccttcgat tgtgggcctc gtgcctgtag gttacgcaga cggctatccg
     2641 cgcgaactgt cgaaccgggg cgaagcgctt gtcaggggag tccggtgccc cgtcgccggg
     2701 cgggtgagca tggaccagat aggtcttgac ctcaccggtg tacccggcgt cgcggagggg
     2761 gatgaggtcg tcctgatagg gcggcagggc ggccaggaga ttaccgccga cgaggtcgcg
     2821 ggctggtgcg gtacgatctc ctacgaaata ctttgcggcc tgcccgaccg cgtgccgcgc
     2881 cactacatcc acgacggcca gcccatcgag gtgtgcaacc tgttagggtg cagccccacc
     2941 cgcgtgaggg ctaaacctcg aaaggagttg agcaggtgag agcgccagaa ttgatcgccg
     3001 ccaagcgaga tggcaaagag cttaccggcg cggaaatcag gtggttcgta gatggcttca
     3061 cgcgcggcga catccccgac tatcagctta ccgcgtggct catggcagtc tacttccagg
     3121 gtatgtcggc ccgcgagacg gcatacctca ccctcgctat ggtggaatct ggccgaaggc
     3181 tcgacctgtc ggtggtcggg ccattcgttg ctgacaagca ctccaccggc ggggtagggg
     3241 ataagaccac ccttgtagta gcccctctcg tggcggcagc aggcgtgccc gttggcaaga
     3301 tgtcgggacg cggcctcggt ttctccggcg gcacgattga caagctggag tcgataaagg
     3361 ggttcagggc gcagcttgag ccggacgagt tcgtagaggg agtgcggcgt gtgggcctgg
     3421 tcgtggcggc ccagacggct gacctcgcgc ccgcagacgg taagctctac gccctgcgcg
     3481 acgtgaccgc gactgtggag agcatccccc tcatcgccag cagcatcatg tccaagaaga
     3541 tagcggcggg cgcgacaggc gttgtgctgg acgtaaaagt gggccgaggg gccttcatga
     3601 agacaatcga ggaggcccgc gaactcgccg tagcgatgcg cgacatcggc accaacgtgg
     3661 gcctacaggt gcgggccgtg ctctccggca tggaccagcc gctagggtgg gcggtaggca
     3721 acgcgctgga agtagccgag gccgtgcaga ccctcaaagg cgaagggccg gaagacctca
     3781 cagaagtggc ctttacgctc ggttcccacc tgttgcacat ggcaggcaaa gtggcagacg
     3841 tcgatgaggg tgtcgagatg atgcgccggg cgctcaactc cggcggcggc ctgcgcaagt
     3901 tccggcagtt cgtcgagaac cagggcggcg acccgtcgtt cgttgacgac cctctcttgc
     3961 tgccccaggc ccccgtgcgc cgccacctct tagcgcagaa tagtgggtct gtttcgtcca
     4021 tagatgccga gataattggc cgcgcctcgg tggaaatcgg ggcggggcgt gcggtgaagg
     4081 gcgcgccaat
//
LOCUS       DASRWZ010000470         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_450043, whole genome shotgun sequence.
ACCESSION   DASRWZ010000470 DASRWZ010000000
VERSION     DASRWZ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450043"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(436..630)
                     /gene="rpmB"
                     /locus_tag="VFH60_07355"
     CDS             complement(436..630)
                     /gene="rpmB"
                     /locus_tag="VFH60_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET6313635.1"
                     /translation="MSVVCDVCGKKPQYGHNVSHAKNHTPRRWVPNVQSKRILVGGEL
                     QRVHICTRCLRTLNKKNAAL"
     gene            759..1238
                     /locus_tag="VFH60_07360"
     CDS             759..1238
                     /locus_tag="VFH60_07360"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET6313636.1"
                     /translation="MTSRNRRDPNHYSLNTAPLSLTGRLNTTQLGNGVIADMLTGQLT
                     EQEEEMGKIEVSPRAISHLASRAAQRSYGVVGLASRHARPGWAELLRRDEVSKGVDVT
                     INEGQVVIDLYVVIEYGTRISEVARNIMSNSKFAVEAALGVDVVQVNVNVQGIRVSK"
     gene            1323..3074
                     /locus_tag="VFH60_07365"
     CDS             1323..3074
                     /locus_tag="VFH60_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET6313637.1"
                     /translation="MTNARQVKGDRDTTEKPHHHLPTGPGETWGGQELRRAVGAAAAW
                     LARNAEAINSLNVFPVPDGDTGTNMALTMQAAVKQVAESPSHSAAEIAAGISHGALMG
                     ARGNSGVILSQILRGFAEGIAGKERISARDFADGLVAATATAYRAVLKPVEGTILTVI
                     KDTGAASHAAGGQLNSYSYVLAETSKAAKASVARTPSLLDKLRDAGVVDAGGQGLYML
                     FDGIRRYADGEDLEADSAEVLVVEAPEMNGAGVQVEHGDYGYCTNFLIVGEGWQFDEV
                     RARIASFGDSAVIVGDERIVKVHIHTETPGTVLNYATSLGKLRQIAITDMQEQHEDFL
                     SGHGLPNAYTVHDDAGALHASGRDVDTATSQSRIALLAVASGPGLVSAFRSMGATAIV
                     EGGQTMNPSTEDLLKVIERVPQNEVIILPNNGNIVMSARQTTSLTKKKVVVVPTDTIP
                     QGMAALIAFNYDASLDDNARAMDSAAKQVETGEITRAVRDAKVEGIEVKEGQIIGLLN
                     NKLVTTGPDREEVAWDLMEKMGAHERELITIYWGGDLTETDAEQFQSRVQEHFANAEV
                     ELVHGGQPFYDYIISAE"
     gene            3211..>3968
                     /locus_tag="VFH60_07370"
     CDS             3211..>3968
                     /locus_tag="VFH60_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET6313638.1"
                     /translation="MPHIRIVTDNACDIPDALLRQFDITVVPHAISWGGSAFSVEGES
                     GGEILARKMKSESGPNAWPMVEAPEVEEFTRLYRSMRDTCDGVLSIHASSKVSDAYGN
                     ALQAREAFGRTGHGGPFPIAVIDSMSMSMGLGWLVLAMYEAAEAGPELQKLASAANRM
                     AGTTHFAFFTESLAGLLKTGHVPRLQAQAEGLSSLKALLHLDEGQLVVYERTRTRPKA
                     RDALYNFVEDFPKIGQIAVLHTGALFDVEHLLTR"
BASE COUNT          813 a         1193 c         1247 g          715 t
ORIGIN      
        1 cgaagccgca ggcccgaggc gcaaacgtcc tttaaagggc atagtcatca caatacccga
       61 aggtcaaggc agcattccaa gaaaagtatt ttactgaagt cagcatgtga taaataacag
      121 cacccaaagg gagaattgtc attccgagcg tagcgaggaa tcaggtgggt aggcgagagt
      181 ccccgacctg gaggatgctg ctagttccag gagctttggt acggatatcg caatagcata
      241 cgactgctac ctggttccga gcgtggcgag gaatgacaat tctccctttg ggtgctcgca
      301 gtgtagtgct cgtcgtgtgg ccttatcctg tcaccgcaga acttggagtc ttgccaacaa
      361 caagagggtc gcctgtcatc gatggggcga ccctcttctt atcgaaacgt gactgctact
      421 gcgtcacgtc tcacactata gcgcggcgtt cttcttgttc agggtgcgca ggcagcgggt
      481 gcagatgtgc acgcgctgta gctcgccacc gacaaggatg cgcttggact ggacgttggg
      541 cacccagcgg cgcggggtgt ggttcttggc gtggctcacg ttgtgcccgt actggggctt
      601 cttgccacac acatcacata ctaccgacat gactattccc ctttacgaaa tcgagcattt
      661 acagtatagt aatgcgtaac atttctcatg cccgtttgca ggccatactc gccaatctta
      721 ccataactgc ccgcgtttgg caaggttatt ttgtccccat gacttccagg aaccgccgag
      781 accccaacca ctatagcctc aacaccgctc cgctctcgct gaccggccgc ctcaacacca
      841 cgcagttggg caacggtgtc atcgctgaca tgcttacggg gcagcttacc gagcaggagg
      901 aggagatggg caagatagag gtgtcgccac gcgctatttc gcacctggcg agccgtgccg
      961 cccaacgctc ctatggtgtg gtgggcctcg cctcgcgcca cgcacgcccc ggttgggccg
     1021 aactactgcg ccgcgacgag gtctccaagg gcgtggacgt gacgatcaat gaggggcagg
     1081 tggtcatcga cctgtacgtg gtcatcgagt acggcacccg catctccgag gtagcccgca
     1141 acatcatgag caactccaag ttcgcagtgg aagccgcgct gggcgtagac gtggtccagg
     1201 tgaacgtcaa cgtccagggc atcagggtta gtaagtgatt tagaatttcg gatttcggat
     1261 ttagtagtaa tctgaacaaa ttcgcaattc taaattccaa tctccaaatc tcttccaaga
     1321 ctatgacaaa cgcacgacag gtcaagggcg accgggatac gaccgagaag cctcaccatc
     1381 atctgccgac cggacctggg gagacctggg gcgggcaaga actccggcgc gcggttgggg
     1441 cggctgccgc ctggctcgcg cgtaacgccg aggcgataaa ctcgctcaac gtcttccccg
     1501 tccccgacgg cgataccggc accaacatgg ccctcaccat gcaggccgcc gtgaagcagg
     1561 tcgcggaatc acccagccat tcggcagccg agattgccgc cgggatctcg cacggtgccc
     1621 tcatgggggc gcgcggcaac tcgggcgtga tactgtcgca gatcctgcgg ggcttcgcgg
     1681 agggcatcgc gggcaaggag cggatttcgg cccgcgattt cgcggacggg ctggtagccg
     1741 ccaccgctac cgcctaccgg gccgtcctca agcccgtgga gggcaccatc ctcaccgtca
     1801 tcaaggacac aggggccgct tcgcacgcgg cggggggcca gctcaactcc tattcctacg
     1861 tgctggccga gaccagcaag gccgccaaag cctcggtggc gcgcacgccc agcctcctcg
     1921 acaaattgcg ggatgccggg gttgtcgatg ccgggggcca ggggctgtac atgctgttcg
     1981 acggcatacg gcgctatgcg gacggtgagg acctggaagc ggacagcgcc gaggtgctcg
     2041 tggtggaagc gcccgaaatg aacggagccg gggtgcaggt ggagcacggc gactacggct
     2101 actgcaccaa cttcttgatc gtgggcgagg ggtggcagtt cgacgaggtg cgcgcccgca
     2161 tcgcctcgtt tggcgactcg gcggtgattg tgggcgacga gcgcatcgtg aaggtgcaca
     2221 tccacaccga gacgccgggc accgtgctga actacgctac ctccctcggc aagctgcgcc
     2281 agattgccat taccgacatg caggagcagc acgaggactt cctgtcgggg cacggcctcc
     2341 ccaacgccta cactgtgcac gacgacgccg gggcgctaca tgcctccggg cgcgacgtgg
     2401 ataccgccac ctcgcagagc cgcatagcct tgctggcggt ggcttcgggg ccggggcttg
     2461 tgagcgcctt ccgcagcatg ggggccacgg ccatcgtcga gggcggccag acgatgaacc
     2521 ccagcacgga ggacctgctc aaggtgatcg agcgggtgcc gcagaacgag gtcatcatcc
     2581 tgcctaacaa cggcaacatc gtcatgtcgg cccgccagac caccagcctc accaagaaga
     2641 aggtcgtcgt ggtgcccacc gacacgatcc cgcagggcat ggcggccttg atagccttca
     2701 actacgacgc gagcctggac gacaacgcca gggcgatgga ctcggccgcc aagcaggtgg
     2761 aaacgggcga gattacgcgg gcggtgcgcg acgccaaagt tgagggcatc gaggtgaagg
     2821 agggccagat catcggcctc ctgaacaaca agctcgtgac caccggcccc gaccgcgaag
     2881 aggtggcctg ggacctgatg gagaagatgg gcgcgcacga gcgcgagcta atcaccatct
     2941 actggggcgg cgacctcacc gagaccgacg ccgagcagtt ccagtcccgc gtgcaggagc
     3001 acttcgctaa cgccgaagta gagctagtgc acggcggcca gccattttac gattacatca
     3061 tttcagcgga gtaatgggtg acgagttagg agttttgaat tttgagttat cagtgaaatg
     3121 gtacgttgat atagctcaat acctgccacg cgtcacccgc ccctgatacc tgacaactca
     3181 taactcataa ctcataactc ttcagagcct atgcctcaca tccgtatcgt cacggacaat
     3241 gcctgcgaca taccagacgc cttgttgagg cagtttgaca ttacggtggt accccacgct
     3301 ataagctggg gcggctcggc gttttcggta gagggtgaga gcggggggga aatcctggcg
     3361 cgcaagatga agagcgagtc ggggccgaac gcctggccca tggtggaagc gccggaggtt
     3421 gaggagttca cccggctcta tcgctccatg cgggatacgt gcgacggggt gctgtcgatc
     3481 cacgctagct ctaaagtgag cgatgcctat ggcaacgcgt tgcaggcgcg cgaggcgttc
     3541 gggcgcaccg ggcacggcgg gccttttccc atcgcggtga tcgattcgat gtcgatgtcc
     3601 atgggcctgg ggtggttggt gctggcgatg tacgaggcgg ccgaggccgg ccccgaattg
     3661 cagaaactgg cgagcgcggc caaccgtatg gcgggcacca cccatttcgc cttcttcacc
     3721 gagagcctgg ccggcctgct gaagaccggg cacgtgccgc gcttgcaggc ccaggcggaa
     3781 ggactcagct cgctcaaggc gctgctgcac ctggacgaag gccaactggt ggtgtacgag
     3841 cgcacgcgca cgcgcccaaa ggccagggac gctctctaca actttgtgga ggactttccc
     3901 aagatcggcc agatagccgt gctgcatacc ggcgcgctgt tcgacgtgga acacctgctg
     3961 acccgtat
//
LOCUS       DASRWZ010000471         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_792821, whole genome shotgun sequence.
ACCESSION   DASRWZ010000471 DASRWZ010000000
VERSION     DASRWZ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792821"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFH60_07375"
     CDS             <1..232
                     /locus_tag="VFH60_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET6313639.1"
                     /translation="MTRWTPSRRNEFIRRLRVLNFDGPYSGTRHQFMVYKQHRLMIPS
                     NAEYSVPQLRFMLRQVEGIIGRPISLADWNNL"
     gene            324..1694
                     /locus_tag="VFH60_07380"
     CDS             324..1694
                     /locus_tag="VFH60_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET6313640.1"
                     /translation="MLQDHQSSIHSNTRSHEWYRRALDVFPSGVTHDNRFFGEVEPIY
                     IDRAHGSRKWDVDGNEYIDYWMGHGALLLGHSHPAIIEAVQSQITRGTHYGGSHPLEV
                     EWGELIKATVPSAERVKFTGSGTEATMMAVRLARAHTRRQKFLKFQGHFHGWSDAMTA
                     GFQAPFDVPSSVGIADSVLQGVIVAPTDLAEVERIFQEHGDDISSVILEPTGASYGTI
                     PLPPGFLQGLRELTGRYNIVLIFDEIVTGFRYTPGGVQAMTGVTPDLTTLAKVVAGGL
                     PGGAVVGRAEVMSAFEFRPGDTEWNRYRRINHPGTFNANPLSASAGIAMLKIAQTGEP
                     QRQIDEMGRRLVSEMNGGLEQLGIEGSCVYGDGPIWHVLLGKGACANRDGTLLEGSAD
                     VLTLRKANDANVKAAFQTGMLGRGVDLLSGHAGIMSTAHTEDDLALTATAFYETLREM
                     RDAKLV"
     gene            1717..2499
                     /locus_tag="VFH60_07385"
     CDS             1717..2499
                     /locus_tag="VFH60_07385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6313641.1"
                     /translation="MKRRKPRHASRPALHPSDGEALALTLLALAYSNLLPWWATRRGL
                     NPESMFRRVNAWLVVLMLAYAARRPGGLARVGLGRCGLSKSVAGGALTGLGLSVPPLI
                     FFYRPILLDTPLEYGPITGMSRREVVEDLFVGVPVKIALLEELLFRGLIFSALRRNHS
                     LPLSLAGASAAFAGWHFSVTYTTAAQTNLSTAARLPGFLKPFVQPLAVAGGMLTTGLA
                     GVAFGLVRERTGNLAGPIVAHWLVDGIMIYSLWRGRPDQTPL"
     gene            2890..>3224
                     /locus_tag="VFH60_07390"
     CDS             2890..>3224
                     /locus_tag="VFH60_07390"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET6313642.1"
                     /translation="MSGTLAAGLAYSIVGSGFPGAFRERRSPLQTAEDDLVRRARLGD
                     QDAFRQLVEVYGTLTERTARVLLADRSDAEDAVQEAWLDAWRGLRGFELGRPFRPWLL
                     TVVGNRCRMV"
BASE COUNT          574 a         1032 c         1007 g          611 t
ORIGIN      
        1 catgacacgt tggacaccca gtaggcgcaa cgaattcatc cggcgcttac gtgtcctcaa
       61 tttcgatggc ccctactccg gtacgcgcca ccagttcatg gtctacaagc agcatcgtct
      121 gatgatacca tcgaacgcgg aatattctgt acctcagttg cgcttcatgc ttcgccaggt
      181 ggaaggcatc attggacgtc ctataagcct agcggactgg aataatcttt agtcttaagc
      241 taaaaaccca ggctctctct aaccgtgatc agcacagctt agagaggaat agcctgggga
      301 ctgccagggt aattatagcc gccatgttgc aggatcatca atcttccatc catagtaata
      361 cccgctccca cgaatggtac cgccgcgccc tcgacgtctt ccccagcggc gtgacgcacg
      421 acaaccgctt cttcggtgag gtcgagccta tctatataga ccgggcgcat ggctcgcgca
      481 agtgggacgt ggacggcaac gagtacatcg actactggat ggggcacggc gcgctcctgc
      541 tggggcacag ccacccggct atcatcgagg cggtgcagag ccagataacc aggggcaccc
      601 actatggcgg ctctcacccc ttggaggtgg agtggggaga gcttatcaag gcgaccgtgc
      661 cctccgccga gcgtgtgaag tttaccggct ccggcaccga ggcgaccatg atggccgtac
      721 ggctggcccg cgcccacacc cggcggcaga agttcctcaa gttccagggc cacttccacg
      781 gctggtctga cgccatgacg gcgggcttcc aggccccctt cgacgtgccc tcctcggtcg
      841 gcatcgctga ctcggtgctc cagggcgtga tcgtggcccc cactgacctt gctgaagtgg
      901 agcgcatctt ccaggagcac ggcgacgata tctcctcggt gatactggag ccgacggggg
      961 cgtcatacgg caccatcccg ctgccccccg gcttcctgca aggtctgcgc gagcttaccg
     1021 gcaggtacaa tatcgtgctc attttcgatg agatagtgac cggcttccgc tacacacccg
     1081 gcggcgtgca ggccatgacc ggcgtgaccc ccgacctgac gaccctggct aaagtggtgg
     1141 cgggcggcct gcccggcggg gcggtggtcg ggcgggccga ggtgatgtcg gccttcgagt
     1201 tccggccggg cgataccgag tggaaccgct accgccgcat caaccacccc ggcacgttca
     1261 acgccaaccc gttgagcgcg tcagcaggta tcgccatgct caagatcgcg cagaccggcg
     1321 agccgcagcg gcagatagac gagatgggcc gccgcctcgt ctcggagatg aacggcgggc
     1381 tggaacagct tgggattgag gggagctgcg tgtacggcga cggccctatc tggcacgtgc
     1441 tgctgggtaa gggggcttgc gccaaccggg acggcacgct gctcgaaggc tcggcggacg
     1501 tgctcacgct gcgcaaggcc aacgacgcga acgtgaaagc ggctttccag acgggcatgc
     1561 tcggccgggg ggtggacctg ctatccgggc acgcgggcat catgtccacc gctcacaccg
     1621 aggacgacct ggccctcacc gccaccgcct tctacgaaac gttgcgcgag atgcgggacg
     1681 cgaagctcgt ctgaggccca cttccaactt cccataatga aacgccgcaa gcctcgccac
     1741 gcctcacgcc ccgcgcttca cccctccgat ggcgaagctc tggccctcac cctgctcgcg
     1801 ctcgcctaca gcaacctgct gccctggtgg gcgacacggc gcggcctgaa cccggagtcg
     1861 atgttccggc gcgtgaacgc ctggctggtg gtgctgatgc ttgcttacgc ggcacgtcgg
     1921 cccggcgggc tggcgcgggt cggcctgggt aggtgtgggc tgtcaaaatc cgtcgcgggt
     1981 ggggcgctca ccggcctggg cctctcggtg ccgcccctga tcttcttcta ccggcctatc
     2041 ctgctcgaca cgcccctcga atatggcccc atcaccggga tgtcgcggcg cgaggtggtg
     2101 gaagacctgt tcgtgggcgt gccggttaag atcgcgctgc tggaggagct gctgttccgg
     2161 ggcctcatct tctccgcgct gcgccgcaat cactccttgc cgctgtcgct ggccggtgcc
     2221 tcggctgcgt tcgcgggctg gcacttctca gtgacttaca ccaccgccgc gcagactaac
     2281 ctctccaccg ccgcccgcct gcccggcttc ctcaagccgt tcgtgcagcc gctggccgtg
     2341 gcgggcggga tgctcaccac ggggcttgcg ggtgtggcgt tcggcctcgt ccgagagcgc
     2401 accggcaacc tggcaggccc catcgtagcc cactggctcg tggacggcat catgatctac
     2461 tccctctggc gcggccgccc cgaccagacc cctctctaaa ctcacgtaca gagacggaaa
     2521 cgccaagacg ccaagcacgc caaggaccac gccaaggtta cgaaaacgca aagcatgccg
     2581 tgagcgcagc cgaagggacg caaagacgcc aaggacgcgc aaagtttaag gattgttgga
     2641 gagtacagca ccttagccgg tccaaagtgt atggttagtg catacggtaa aagtgaagtt
     2701 tccttgggta ttacttaaga ggaaacttca cttttggaga gggagcaggg tacttcggca
     2761 tgtaccctgc tcctctttgt ttacaacacc tacttactct tggcgtcctg gcgtttagaa
     2821 gacttgtctc ctgcccctgt gatgttgtca cgagggcggg gttgtatata cttaagccaa
     2881 tccggtgtga tgtccggaac tttggcggcg ggtcttgcat attctattgt gggaagtggt
     2941 ttccccggcg cttttcggga gaggagaagc cccctgcaaa cggcagaaga cgatctcgtc
     3001 aggcgtgcgc gcctgggcga tcaggatgcg ttccgccagt tggtggaagt gtacggtacg
     3061 cttaccgagc gtaccgcgcg tgtgctgctc gccgataggt cggatgccga agatgccgtg
     3121 caggaggcct ggctcgacgc ctggcggggg cttcgaggct tcgaacttgg caggcccttc
     3181 cggccctggc tgctcaccgt cgtgggcaac cgctgccgca tggt
//
LOCUS       DASRWZ010000472         1827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_724247, whole genome shotgun sequence.
ACCESSION   DASRWZ010000472 DASRWZ010000000
VERSION     DASRWZ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724247"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFH60_07395"
     CDS             <1..632
                     /locus_tag="VFH60_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6313643.1"
                     /translation="RTSNDFRRCVQLGRRPLVNIVNVGYDSTNYYVLADTHPRLLVDA
                     GWPGTLPRFRHACERMGVSLPDIKHFLVTHYHPDHAGLAQELKRLGLKLIIVDTQVSA
                     IPVLRSYTKPRDNFVDIDLVDNIVLSVEESRAFLAGIGIQGEIISTPGHSDDSITLVL
                     DEGAAFTGDLTPPMMVPDDGENPARQSWETIRSLGVTTIYPGHGPVGHI"
     gene            730..>1827
                     /locus_tag="VFH60_07400"
     CDS             730..>1827
                     /locus_tag="VFH60_07400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6313644.1"
                     /translation="MAAIAVWAMRLRMRTLERMPRPHRPFWSAFSAGLTLLAVSAVYP
                     HMERATGLAIVLPVAEAATVAGMLAWLVAVLLACRYVPLTVRAGLLLAALAIVTACAV
                     LLERGAGVLDWAEDASGLDTKYAAIATSAGGLTWNVAFLASVLALGAAIVLVRYLAEG
                     PLPNSHAIAIGAMLAGILYSGWDWLGAGQDEVVLASGQVLAALLAADAVYTAIRRGRR
                     GDIPSDDRSAWRGTSPWLPFLAGLVLVLLAFEHIVPASFLRFYPAETAYNRGIVFVGA
                     VLGMLLVLVRGAAATVEAERLRHKHSQLVGQNEEYVRLAISDSLTQLFNKGYFEYRLR
                     LECDRSRRNQQPLTLIALDLDNFKQVNDRHGH"
BASE COUNT          302 a          576 c          604 g          345 t
ORIGIN      
        1 tgcgcacctc caacgacttc cgaagatgcg tccagttggg taggagacct ctggtgaaca
       61 tcgtcaacgt cggctacgac tccaccaact actacgtgct ggcggacacc cacccccgcc
      121 tgctggtaga tgcgggctgg cccggcacgc tccccaggtt ccgccacgcc tgcgaacgca
      181 tgggcgtatc cctccccgat atcaagcact tcctggtgac gcactaccat cccgaccacg
      241 ccgggctggc gcaggagctg aagcgcctcg gcctcaagct gatcatcgtt gatacccagg
      301 tctcagccat cccggtcctg cgcagctata cgaaaccgag ggataacttt gtcgatatag
      361 atctggtgga caacatcgtg ctcagtgtgg aggagagccg cgctttcctg gcgggtatcg
      421 gcatccaggg tgaaatcatc agcacgccgg ggcattcgga tgatagtatt acgttggttc
      481 tcgatgaagg cgcggccttt acgggcgacc tcaccccgcc catgatggtg cctgatgatg
      541 gcgagaaccc ggcccggcag agttgggaga cgattcggtc cctcggtgtg acgaccattt
      601 acccgggaca cgggccggtg gggcacatct aggcacgcgc cgcttagttc gtacatttgc
      661 ggcgcaggca cccggcccga aatatagagg ctatcctcca tgacgctagc ggcagcactg
      721 gtactgagtt tggcggctat agctgtgtgg gccatgcggc tgcgcatgcg gacgctcgag
      781 cgcatgcccc ggccccatcg ccctttctgg agcgccttct ccgccgggct gaccctactc
      841 gccgtaagcg ccgtctaccc tcacatggag cgcgctaccg ggctggctat cgtgctgccg
      901 gtggcggaag cggctaccgt ggcagggatg ctggcgtggt tggtcgccgt actgctcgcc
      961 tgccgctacg tgccgctgac cgtccgcgcc gggttgctcc tcgcggccct ggctatagta
     1021 acggcgtgcg cggtgctgct ggaacgcggg gcgggggtgc tggattgggc tgaggacgcc
     1081 agcggccttg atacaaagta cgccgccatt gccacgtctg ccggggggct gacctggaac
     1141 gtggctttcc tggcatccgt gcttgcgctg ggcgcggcta tcgtcctggt gcgctacctg
     1201 gcggagggtc cgctgcccaa ctctcatgcg atagccattg gcgcgatgct ggcgggcatc
     1261 ctgtactcag gctgggactg gctgggcgcg gggcaagatg aggtcgtgct ggcgagcggg
     1321 caggtgctgg cggccctact ggcggcagac gccgtctaca ccgccatacg gcgcgggcgg
     1381 cgaggcgata taccctccga cgacaggagc gcatggcggg gcacgtcacc ctggctgcct
     1441 ttcctcgcgg ggttggtact cgtgctgctg gcctttgagc atatagtgcc ggcctccttc
     1501 ctgcgcttct acccggctga gaccgcctac aatcgcggga tagtgttcgt gggggcggtg
     1561 ttgggcatgc tgctcgtgct ggtacggggt gccgccgcta cggtcgaggc cgagaggctg
     1621 cggcacaagc actcgcagct ggtgggccag aacgaggagt acgtcaggct ggcaatcagc
     1681 gactcgctga cccagttgtt caacaagggc tacttcgagt acaggctcag gctggagtgc
     1741 gacaggagca ggcgcaacca gcaacccctc accctgatag ccctcgacct ggataatttc
     1801 aagcaggtaa acgaccgcca cggccac
//
LOCUS       DASRWZ010000473         5472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_655657, whole genome shotgun sequence.
ACCESSION   DASRWZ010000473 DASRWZ010000000
VERSION     DASRWZ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655657"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFH60_07405"
     CDS             complement(<1..214)
                     /locus_tag="VFH60_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313645.1"
                     /translation="MLAPLWYPVSRDAPGGIETFLYSLIEALEREGCSVTLLASGDSR
                     AAGELVPVVERNLYSLMHEGSAGEYVY"
     gene            complement(223..1374)
                     /locus_tag="VFH60_07410"
     CDS             complement(223..1374)
                     /locus_tag="VFH60_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6313646.1"
                     /translation="MNIIVAGVMGRYPYGGVAWCSLMYLLGLMRLGHRVWYLEDMCEC
                     NFDPVENTLSKDPRYALNFIRDCLQPYGLEDRWCYVDWRQGYHGHTREQWLDVCAGAD
                     LLINLSGGCAFWRDEYAAIPHSAYIDTDPGFTQLDAERRPERVDFLSRYGALFTFGRN
                     IGTPASPVPALDLQWEHTWQPVLVDHWRPTGETPRNAFTTVMTWRIASFEEIGGNKDQ
                     ELLRFLDLPRHASTPLELAVNGPRRFLEAHGWVCRDAFEVSHSPGVYRDYISSSLGEF
                     SVAKHTYVATNSGWFSDRTACYLASGRPAVVQDTGFSAHLPSGSGLLAFRTFQEAQDG
                     LENVAAGYSRHAKAARDLAVACFAPEVVLPPLLECATQNKSTTRKAQAV"
     gene            complement(1371..3212)
                     /locus_tag="VFH60_07415"
     CDS             complement(1371..3212)
                     /locus_tag="VFH60_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313647.1"
                     /translation="MRARPASGSAGTLTVCLWMLRYASRRWQGMLAMLAVVLLGVGVG
                     VVRPWPMKVLVDNVLQGRPLDPAGGAIMGVLPGASDQGGLLAWTVGATLVIFFAGWLL
                     NLARAYTNIGFGQRLKYDLAADLFRHLQRLSLRFHSRKPLGDSIHRVTSDSESIAIIV
                     KDALIPTLSAVFSLCAVFGIMWSLDPLLTMLALLVVPALALVLKLYAEPMMRHSYAQQ
                     ETEGRMYTDVEETLGALPVVQAYAAEDRADMRLRDTTDEVLDTTLRTTRVQLQFKVLS
                     GLVTALGTALLIYVGAQHALDGTLTVGSIIVFLSYLASLYAPISSLMYTSSTVQSAMG
                     SARRVIEVLSTEQEVKDRPGARPLPPVRGHVALDNVTFGYVKGNPVLKDVSLDVQPGE
                     TVALVGTTGAGKSTLAGMVARFADPWTGRVRIDGRDVRAVTLRSLREQVALVGQEPVL
                     LPITVAENIAYGRPEASMEEIVAAAKAANAHSFIERLPEGYDTVVGERGATLSGGERQ
                     RISIARALLKDAPILILDEPTSAIDAQTEHEILNALRNLVRGRTTLMITHRLSTAALA
                     GRIAVMQGGRIVEQGTHQELVARGGAYARLYAAQHGDDGALGLPEAG"
     gene            complement(3193..>5472)
                     /locus_tag="VFH60_07420"
     CDS             complement(3193..>5472)
                     /locus_tag="VFH60_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313648.1"
                     /translation="PRVRRLSVWRESWQPPEAWLEWIEADIKERGGTVQRGGDYDNWD
                     LKAQGGLTGSARLLMAIEEHGSGQQLARFRIWPRVGRPGLWPLLLLTLLCAGSALSGA
                     WVAFAGLAAMLVLLAARVLYDCASGVETLRVAVRSSPGTHLDKAGKPKRPRPYRMLLS
                     YARPYWRAWLFIAILTTLSTAFTLLEPWPMKVLVDNVLGGHALQGPVGDLAARLPGAT
                     GREGLLWWVVAAGVLVFLGSSALDALLTLKWVRAGQGMVYTLARTLFARIQRRSLVFH
                     SRVPVGDSLSRVTGDAWVVNTAADKLLLAPAQAAITTAIVIAIMLPMNLTLTVLAVAV
                     TPFMAVSSLLLSGRIKALSRVRRQTESRMQAHVQRILGSISVVQAFTREDQEQRRFKE
                     YARKTIHVQQQSALMSSLYNLGSGLPAAAGAALVLWVGAGQVMSGRLTVGELLVFVAY
                     MGVLQAQLKTFAGINRAMQEATGSAERVSEVLATNDEVRDRPGAPELADVRGHIRLAN
                     VNFGYDAKRPVLRDFSLEIEPGQMVAVVGQSGAGKSTLAGLISRLYDPQQGRVEVDGQ
                     DVREVSLRSLREAVAIILQEPFLLSGTIAENIAYSRPDAEPSEIKAAAEEANADSFIE
                     RLPEGYDTELGERGATLSGGERQRISIARALLKDAPILILDEPTSALDARTEQSMLEH
                     LRRLREGRTTIIITHRLSAARAADRTVVLHKGRLVEQGTHDELMARGGLYRRMYNLQS
                     GSRPTVEALRFGSSESAAR"
BASE COUNT          945 a         1883 c         1727 g          917 t
ORIGIN      
        1 agtagacgta ttcgcccgcg ctcccttcgt gcatcaggct gtagaggttg cgctcaacca
       61 cgggcaccag ctccccggcc gcacgagagt cgccgctggc gagcaacgtc accgagcaac
      121 cctccctctc tagggcctct atcagagagt aaaggaaagt ctcgatgccc cccggcgcgt
      181 ccctggagac cgggtaccac aacggggcca gcatgagcgc tctcatacag cctgcgcctt
      241 ccgcgtagtg ctcttgttct gtgtggcgca ctcgagcagc ggaggcagca ccacctccgg
      301 cgcgaagcac gcgacagcca ggtcgcgcgc cgcttttgcg tgtcgggagt agccggcagc
      361 cacgttctcc aggccgtcct gggcctcctg gaacgtcctg aacgctaaca ggccgctacc
      421 ggacggcagg tgcgcgctga agccggtgtc ctgcaccacg gccgggcggc ccgatgccag
      481 gtagcaggcc gtgcggtcgc tgaaccaccc gctgttggtg gccacatagg tgtgcttggc
      541 gacgctgaac tcccccaggg aggaggagat atagtcgcgg tagacgccgg ggctgtgcga
      601 cacctcaaaa gcgtcgcggc acacccagcc gtgcgcctca aggaagcggc gcggaccgtt
      661 caccgcaagc tccaggggcg tgctcgcgtg gcgcggcaga tcgaggaaac gcagtagctc
      721 ctggtccttg ttaccgccga tctcctcgaa gctggctatc ctccaggtca tcaccgtggt
      781 gaaagcattg cgtggcgtct cgccggtggg ccgccagtgg tctaccaaca ccggctgcca
      841 ggtatgctcc cattggaggt cgagcgcagg cacgggagag gcaggcgtgc cgatgttgcg
      901 gccaaaggta aacagcgcgc cgtaacggga aaggaagtcc acccgctccg gccgccgctc
      961 ggcgtctagc tgcgtgaagc cagggtctgt gtcgatatac gcagagtgcg ggattgcggc
     1021 gtactcgtcg cgccagaacg cacagccgcc tgagaggttg atcaacaggt ccgcgcccgc
     1081 gcaaacgtcc agccactgct ccctggtatg cccgtggtag ccctgccgcc agtccacgta
     1141 gcaccagcgg tcttccaggc cgtaaggttg caggcaatcg cggatgaagt tgagcgcgta
     1201 gcgggggtcc ttcgacaggg tgttctccac cgggtcgaag ttgcactcgc acatgtcctc
     1261 caggtaccac acccggtggc ccaggcgcat cagccccagc aggtacataa gcgagcacca
     1321 ggccactccc ccgtaagggt agcgacccat gacccccgcg acgatgatgt tcatcccgcc
     1381 tccggtaggc ccagggctcc atcgtcgccg tgctgcgcgg cgtacagcct ggcataagcc
     1441 ccacctctcg cgacaagctc ctggtgggtg ccttgctcca caatcctgcc gccttgcatg
     1501 acggcgatgc gaccggccag ggctgcggta gagagccggt gcgtgatcat cagggtagtc
     1561 cttccccgta ccaggttgcg caaggcgttg agtatctcgt gctcggtctg ggcatcgatg
     1621 gcgctggtcg gctcatcgag gatgaggata ggggcgtcct tgagcaaggc cctggctatg
     1681 gagatgcgct ggcgctcgcc tcctgagaga gtggccccgc gctctccaac cacggtgtcg
     1741 tagccctccg gcaggcgctc gatgaagctg tgggcgttgg cggcctttgc cgccgcgact
     1801 atctcctcca tgctagcttc ggggcggccg tacgcgatgt tctcggctac cgtgatgggc
     1861 agcagcacgg gttcctggcc caccagggcc acctgctcgc gcaggctgcg gagggtcact
     1921 gcgcgtacgt cgcggccgtc tatgcggacc ctgcccgtcc atgggtccgc gaaacgagcc
     1981 accatgcccg ccagcgtgct cttgcccgcg cctgtcgtgc ccacaagggc taccgtttcc
     2041 cccggctgca cgtccaggga cacgtctttg agcacggggt tgcccttcac gtagccgaaa
     2101 gtgacattgt cgagcgcaac gtgcccccgt accggcggca atgggcgggc acccggcctg
     2161 tccttcacct cctgctccgt gctgagtacc tcgatcaccc ggcgggcgct gcccatagcg
     2221 ctctgcacgg tcgaagaagt atacatgagc gaggagatag gcgcgtacag cgaagccagg
     2281 taagacagga agacgatgat gctgcccacg gtgagagtac cgtcgagggc gtgctgcgcg
     2341 cccacgtaaa tcagcagggc cgtgcccagg gcagtcacca gccccgacag caccttgaac
     2401 tgcaactgca cgcgggtcgt gcgcagggta gtatccaata cctcgtcggt ggtatcgcgc
     2461 aggcgcatgt cggcccggtc ctccgccgcg tatgcctgca ccaccggcaa ggcccccagg
     2521 gtctcctcaa cgtcggtgta catgcggccc tcggtctcct gctgtgcgta gctgtggcgc
     2581 atcatcggct cggcatacaa cttcaacacg agggcgaggg cgggcaccac cagcagggcc
     2641 agcatggtga gcaaggggtc caggctccac atgatcccga ataccgcgca caggctgaac
     2701 accgccgaca gggtcggtat gagcgcatct ttgacgatga tggcgatgct ttcgctgtcg
     2761 gaggtgaccc ggtgtatcga gtcgcccagg ggcttgcggc tgtggaatcg tagcgacaga
     2821 cgctggaggt ggcggaaaag gtcggcggcc aaatcgtact tgaggcgctg cccgaaaccg
     2881 atattggtat aggcgcgcgc caggttcaac agccagccgg cgaagaagat taccagggtg
     2941 gcacccaccg tccaggccag caggccccct tggtcggagg cccccggcag tacccccatg
     3001 atggccccac cggcggggtc gagcggcctg ccttgcaaca cgttgtccac gagcaccttc
     3061 atgggccagg gcctaaccac gccgacgccg acccccagca gcacgacggc gagcatagcc
     3121 aacataccct gccagcggcg ggaggcgtac ctgagcatcc acaggcagac cgtgagggtg
     3181 cccgcggagc cgctagcggg ccgcgctctc actgcttcca aagcgcaatg cctccactgt
     3241 gggcctggac cccgactgca ggttgtacat cctgcggtac agccctcccc ttgccatcaa
     3301 ctcgtcgtga gtgccctgtt ccaccagtct gcctttgtgc agcaccaccg tcctgtccgc
     3361 ggcccgcgcg gcggacaggc ggtgcgtgat gataatggtt gtcctgccct ctcgcagccg
     3421 ccgcaaatgc tccaacatac tctgctcggt gcgggcgtcg agggcgctgg tcggctcatc
     3481 gagaatcagt atgggcgcgt ccttgagcag cgccctcgct attgagatgc gctggcgctc
     3541 gcccccggag agagtcgccc cacgctcgcc cagctctgtg tcgtaaccct ccggcagcct
     3601 ctcgatgaag ctatcggcgt tcgcctcctc ggcagcggct ttgatttcgg acggctccgc
     3661 atccgggcgg ctgtaggcta tgttctcagc gatggtgccc gacaggagga aaggctcctg
     3721 cagtatgata gctaccgcct cccgcaggct ccgcaggctc acctcgcgca cgtcctgccc
     3781 gtccacctcc acacgcccct gttgcgggtc gtacagccgc gagatcagcc cggccagggt
     3841 gctcttaccc gccccggact gccctaccac ggccaccatc tgcccaggct ctatctccag
     3901 agagaagtca cgcagcacgg gccgcttggc gtcgtatccg aagttgacgt tggccaaccg
     3961 tatatgcccc ctcacgtcgg ctagctccgg tgcgccgggc ctgtcccgca cctcgtcgtt
     4021 cgttgccagc acttccgaca cgcgctcggc gctgcccgtg gcttcctgca tggccctgtt
     4081 gatcccggcg aacgtcttga gttgggcctg cagcacgccc atgtaagcaa cgaacaccag
     4141 tagctcgccc actgtaagtc tgccgctcat cacctgcccc gcgcccaccc acagcaccag
     4201 cgccgccccg gctgccgcag ggaggcccga gcctagattg tatagactcg acatgagggc
     4261 gctttgctgc tggacgtgga tggtcttgcg ggcgtactcc ttgaagcgtc tttgctcctg
     4321 gtcctcccgc gtgaaggcct gcaccaccga gatgctgccc aggatgcgct ggacgtgggc
     4381 ctgcatacgg ctttcggtct ggcggcgcac cctgctcagg gccttgatgc gacccgaaag
     4441 cagcagggat gatacagcca tgaaaggggt caccgccacc gccagcacgg tcagggtcag
     4501 gttcatcggc agcatgatgg caatgacaat cgccgtagta atcgcggcct gcgcgggcgc
     4561 cagcaacaac ttgtcggcag ccgtattcac aacccaggca tcgccggtga cgcggctcag
     4621 ggaatcgcct acgggcacgc ggctgtggaa caccagcgaa cgccgctgta tgcgcgcgaa
     4681 cagggtgcgg gccagcgtgt acaccatgcc ctgcccggcg cgcacccact tgagggtaag
     4741 cagggcatcg agcgcgcttg agcccaggaa caccagtact ccggccgcca ccacccacca
     4801 cagcagtccc tcccggccgg tagcccccgg cagccgggcc gccaggtcac ctacgggacc
     4861 ctgtaatgca tggccgccga gcacgttatc caccagcacc ttcatgggcc agggttccag
     4921 caacgtgaac gcggtgctca gcgtggtcag tatcgcgatg aacagccagg ccctccagta
     4981 agggcgggcg tagctcagca gcatgcggta agggcgtggt cgcttcggct ttccggcctt
     5041 gtcaaggtgg gtacccggtg acgatctcac cgccacgcga agagtctcca ccccagaggc
     5101 gcaatcgtac agcactcgcg ctgccaggag cacgagcatg gcggccagcc cggcgaaggc
     5161 cacccacgcg ccgctcaggg ccgagccggc gcacaggaga gtcaggagca acaacggcca
     5221 gaggccaggc cgtccaactc gcggccagat gcggaacctc gccagttgct gcccgctgcc
     5281 atgctcttct atcgccatga gcaggcgggc cgagccggtg agtcctccct gggctttgag
     5341 gtcccagttg tcgtagtcgc cgccccgctg cacggtgccg ccccgctcct tgatgtcagc
     5401 ttcgatccat tccagccacg cttcgggcgg ctgccagctc tcgcgccaga cggagagccg
     5461 gcgcaccctg gg
//
LOCUS       DASRWZ010000474         3205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_244173, whole genome shotgun sequence.
ACCESSION   DASRWZ010000474 DASRWZ010000000
VERSION     DASRWZ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244173"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1987
                     /locus_tag="VFH60_07425"
     CDS             <1..1987
                     /locus_tag="VFH60_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6313649.1"
                     /translation="ACIPQPGTTQQLEAITDRLMYRLAYRNFGTHESLVVNHTVDAQG
                     TGQAGIRWYELRNPNTTLTVHQQGTYAPDTSHRWMGSMSMDGDGNMALGYSVSNGTDV
                     FPAIRYTGRLATDPLNLMAQGEATLYPGYGSQTANARWGDYSMMGIDPVDDCTFWYTQ
                     EYYGATGQYWQTRVGKFRFASCGGGAIPTETPMPAPDPCTLPGVKLVTDSTGDAADIT
                     GAVDTEGTEKYDIQWISIAEPYTATGPSHLAFTMKVASLAGPDNSPSASPNSAWRMYF
                     TSPMSTTVRFVDMRTDNSGVVTYKYGSMTGAAGTTHTTEGDADAGVYGFDGTIRITVA
                     TSKLGNLAPGQTLSAVHGRVFIMTPTGGFYSPDTANYPEPDKSVTYTLVGNASCQPVV
                     EATPTTTGTAMPSSTSAATATRTSTSVPGTTSTPVASSTPNATATACPIEFADVPATG
                     EGSTFYPYVRCLACRGIVSGYPCGGPGEGCNGNDDPYYRPSANVTRGQLSKIVANSAG
                     LDDPIAEGQQQFADVEPGDPFYEFVERLAQTGAIAGYPCSGQGEGCDEQNRPYFRPGN
                     FATRGQISKIVAIAAGFEEEIPEDRKTFTDVPADSPFWVYIERLAGRDVISGYGEAEK
                     CPTGTPCFLYNDYTTRGQMEKIAANAFFPNCVTPARK"
     gene            2637..>3205
                     /locus_tag="VFH60_07430"
     CDS             2637..>3205
                     /locus_tag="VFH60_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313650.1"
                     /translation="MSKIKRKALYLVITCILLTAITAGASGSRSSAAQTLPGNASRTF
                     PETGKTVRGIFLEYWDRNGGLPQQGFPISETMAEVSDLDGKSYTVQYFERAVFEHHPE
                     NQPPYDVLLSQLGTFQYRAKYASGGGATPSPSPQASQPIDGLFDAGGHKLYINCDGEG
                     SSTVLMEAGLGTASSTWSLVLPDLRKLTRV"
BASE COUNT          631 a          999 c          980 g          595 t
ORIGIN      
        1 agcttgtatc ccacagccgg gtaccaccca gcaactcgaa gccataacgg accggctgat
       61 gtacaggttg gcctatcgca acttcggcac tcacgagtcc cttgtagtga atcacactgt
      121 agacgcccag ggcacgggcc aggcgggcat tcgctggtac gagctgcgca accccaatac
      181 tacactcacc gtccaccagc agggcaccta cgcccccgac acgagccatc ggtggatggg
      241 cagcatgtct atggacgggg acggcaacat ggccctcggc tacagcgtgt caaacggcac
      301 ggacgtcttc ccggccatac gctacaccgg caggctggcg accgacccac tcaatcttat
      361 ggcacagggt gaagccaccc tctaccccgg ttacgggtcg cagaccgcca acgcccgctg
      421 gggcgactac agcatgatgg gcatcgaccc ggtggacgac tgcacattct ggtataccca
      481 ggaatactat ggcgctaccg gccagtactg gcagacgcgc gtgggcaagt tccggttcgc
      541 aagctgcgga ggtggcgcga tacccaccga gacccccatg cccgcccccg atccctgcac
      601 gctgcccggc gtcaagttgg tgactgattc gacgggcgat gcagcggaca ttacgggcgc
      661 tgttgacact gagggtacgg agaaatacga catccagtgg atctctatag ccgaacccta
      721 cacggcgacg gggccgagcc acctggcgtt caccatgaag gtggcaagcc tggccgggcc
      781 ggataactcc ccctctgcca gccccaactc ggcgtggcgc atgtacttca ccagcccgat
      841 gtcgactacc gtccgcttcg tggacatgcg caccgacaac agcggcgtgg tgacctacaa
      901 atacggtagc atgacaggcg cagcgggcac aactcacact acagagggcg atgccgacgc
      961 gggcgtgtat ggttttgacg gtactatacg gattaccgtg gctacctcca agctgggcaa
     1021 tctcgcgccc ggccagacgc tctcggcggt ccacggcagg gtgtttatca tgacgcccac
     1081 gggcggtttc tactctcctg ataccgccaa ctatcccgaa cccgacaaga gcgtgacgta
     1141 cacgctcgtc ggcaacgcct cctgccagcc ggtcgtagaa gccacaccta ccactacggg
     1201 taccgcgatg cccagcagca cgagtgcggc cacggccact cgcacctcta cctccgtgcc
     1261 gggcaccacc tctacccctg tagccagcag cacgcccaac gccacggcca ctgcctgccc
     1321 gatcgagttt gccgatgtgc cggccacggg cgaaggctct accttctacc cgtacgtgcg
     1381 ctgtctcgcc tgccggggca tcgtgagcgg gtacccctgc ggcgggcctg gagaggggtg
     1441 caacggcaat gacgacccct actacaggcc gagcgcgaac gtcacacgcg ggcaactgtc
     1501 gaagatcgtt gccaactcgg cggggctgga cgacccgata gcggagggcc agcagcagtt
     1561 cgcggacgta gagccgggtg acccgttcta tgagttcgtg gagaggctcg cccagacggg
     1621 tgccatagcg ggctacccgt gcagcgggca gggagagggg tgcgacgagc agaacaggcc
     1681 ctacttccgc cccggcaact tcgccaccag gggccagata tcgaagatag tggcgattgc
     1741 ggcaggcttc gaggaggaga tacctgagga ccggaagacg ttcaccgacg taccggcaga
     1801 tagtcccttc tgggtgtaca tcgaacgtct ggcgggtaga gacgtaattt ccggctacgg
     1861 cgaggctgag aagtgcccga cgggtactcc ctgcttcctt tacaacgact acaccacacg
     1921 aggccagatg gagaagatag ccgctaacgc cttcttcccc aactgcgtca ctcccgcccg
     1981 gaaatagaac gctacaggca tctctagtag tggaaaagca gcatggaagg cagcagtagt
     2041 atcgtggcga tactcctgct gcttcataga gaccgggtag ggacccctag gagtgcttac
     2101 gcctattatt ctctctattt tgatattcgg ggcttttgcc ctggcgttcg acggtgtctg
     2161 gtccgttgtt gcgctgcgga ccgggtctcg gtatggacgc ctggcctggg tgaactttct
     2221 catctatttt gcggccggct tcgtggcagg ttcgggcgcg catggcatgc cgaatgggct
     2281 gatggccgga atgctggtcg gaggggcggt ttcctctatt gatgccacat tgggttggtg
     2341 gctatcagcg gtggtgggac cgggccgacc gccaccaggg ctgccctttt ttaggatcgc
     2401 cctcgtggcc atggtggttg ttatttatgg ggctttgtcc ggagccattg ggggtatgct
     2461 gtcagagctg gtatagcaac cctgggactg gattcgcttc aggccagctt gaaccaggtc
     2521 gcggactcca ccgcttcgtg accggaacga atcaacttca cttcgcatat taaggatagt
     2581 cttgtgcgcg caggacggcg gttgtgaacc cttatcatgc atggggagag ttgaccatgt
     2641 ccaaaatcaa gcgcaaggca ctttacctcg ttatcacctg tattttgctg acggccataa
     2701 cggcgggcgc gtccggctcg cgatcctcgg cggcgcagac tttgcccggc aacgccagcc
     2761 gcaccttccc cgaaacaggc aagacggtgc gcggcatctt cctcgaatac tgggacagga
     2821 acggtggcct cccacaacag ggcttcccca tctccgagac tatggccgag gtgtcggacc
     2881 tggacggcaa gagctacacg gtgcagtact tcgagcgagc ggtgttcgag caccaccctg
     2941 agaaccagcc cccttacgac gtgctactgt cgcaactggg caccttccag tacagggcca
     3001 agtacgcgag tggcggcggg gctacacctt cgccttcgcc acaggcgagt cagccaatcg
     3061 acggcctgtt cgatgcgggc gggcacaagc tgtatatcaa ctgtgatgga gagggcagct
     3121 ctaccgtgct catggaggcg gggctaggta cggcctcctc cacttggtcg ctcgtgttgc
     3181 ctgatttgcg gaagctgacg cgggt
//
LOCUS       DASRWZ010000475         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_518917, whole genome shotgun sequence.
ACCESSION   DASRWZ010000475 DASRWZ010000000
VERSION     DASRWZ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518917"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..>3230
                     /locus_tag="VFH60_07435"
     CDS             229..>3230
                     /locus_tag="VFH60_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6313651.1"
                     /translation="MSNRAWSYIWTVLLVGAACIAWSLLAFDFTAAQPQLITFLALTG
                     FATVSQLFEASHGKQSYYPHFVFFLAGVFLLEPLLFMMVIVLPHLLEWAKERFTGSDH
                     LRNWYLQPFNIATHVIAACGAWWTHHTINTRMTGMVDPVFSLPVVPVLSAVCAVLVYV
                     VLNHALVGLALALARNTSWKQSGVMKWQSLQPDFIMSCLGYVVAELWHVNPWLVLPAL
                     SPLVLMYQALMVPQLRHEAQTDSKTGLHNARHFSTLYQSEIERTKRFGRPLALIMADL
                     DLLRNINNTYGHLAGDAVLAGIGRIIRDTVRDFDIAGRFGGEEFAIVLPEATPEEARA
                     TAERLRAAVEAASFSVPNNTTQLHVTMSVGVACFPIDAETPTDMIHRADIAVYQAKLN
                     GRNCVVCGSDIPQSIDFAAAPSDRLSAPYSGTFVPRPTTNGNGAHRNGANGLHGSAMP
                     SATLAARPSSAPAKVPVAPVTLPVGRPPLRPNTAPIRAEGTAPLISPLTAALAPGAVI
                     SAAITHPITHPITEPQLKAEKYRSHVRESSPSVPSGLQKAVFPMFIASVILAGFAFAF
                     TGFFMHAAPDPMAIGLFVMLAVGAELLQLDLYGKGTVSVSVAVAFAAACVTGLPGLAL
                     VSAAIVLAHRLRQTQTPILDKTSAYKTAFNWATHLLAGVSPVFALRLLHIEFRLDNLP
                     MLIAPMLIAAMAYFIVDTGLIATVIGLVSGENPVKVWRDRYQWLVSHYLTLCTLGLFV
                     GLAYMTSGPLGVVVFTMPVLMMRVAMKQYISQTEHSVRELQRMNEQLTHANAEVVSAS
                     RTMQSLNEELFLTLSKIIDARDPYVGGHASKVGDYAVAIAHDLGVDPERFEPLRQAGF
                     LHDIGKIAISEAVLHKPGKLTDEEYEYIKTHAALGGEFLEMCRGLRHLAPFVRHHHER
                     WDGKGYPDKLEGEVIPLEARILAVCDAVEAMASDRPYRKGMSLDEVIAEVKRCAGTQF
                     DPHVAAAFVRIAERERDHLIVNSATEVHR"
BASE COUNT          560 a         1113 c          956 g          601 t
ORIGIN      
        1 tacccctatc atgtgattgt tatccacacg agcgtgccat aatataagtt agtaagagag
       61 acgcggggtt tagtaaacct tgtgtgtctc gttgggctaa gcaattttgc ggcgggcttt
      121 gggagctttc tctatcgacc gcaccgcgca cggtatgctg ggagcgctct gccgtgcaac
      181 gcgggaccgc gaggcccaga aaatcttcag agggcggaga caagggtcat gtcgaacagg
      241 gcttggtcat atatctggac ggttctcctc gtaggggccg cgtgtatcgc gtggtccttg
      301 cttgcgttcg acttcaccgc cgcccagccg caactcatta ctttcctcgc gctcaccggc
      361 ttcgctactg tatcgcagct cttcgaggcc tcccacggca agcagtctta ctacccgcac
      421 ttcgtattct tcctcgcggg cgtctttctg ctcgaaccgc tcctgttcat gatggtcatt
      481 gttctgcccc acctgttaga gtgggccaag gaacgcttca ccggcagcga ccacctgcgc
      541 aactggtacc ttcagccctt caacatcgcc acccacgtca tcgcggcgtg cggcgcgtgg
      601 tggacccacc acaccatcaa cacccgcatg acgggcatgg tagacccggt cttctcgctg
      661 cccgtggtgc cggtgctgtc cgccgtgtgc gcggtgctgg tgtatgtggt actcaaccac
      721 gccctcgtcg gcctggccct ggcgctggcc cgcaacacct cgtggaagca gtcgggagtc
      781 atgaagtggc agagcttgca gcccgacttc atcatgtcgt gcctgggcta cgtggtggcc
      841 gagctgtggc acgtcaaccc gtggctggtg ctcccggcgc tctcgcccct ggtgctcatg
      901 taccaggctc tcatggtgcc ccaactaagg cacgaggcgc agaccgacag caagaccgga
      961 ctgcacaacg cccgccattt cagcaccctc taccagtcag agatagagcg taccaagcgt
     1021 ttcggccggc cccttgccct catcatggcc gacctcgacc tgctgcgcaa catcaacaac
     1081 acctacgggc acctggcggg cgacgccgtg ctggcgggca tcggtcgcat catccgggac
     1141 accgtgcgcg atttcgatat agcggggcgc ttcggcggcg aggagttcgc catcgtcctg
     1201 cctgaagcca ctcccgaaga ggccagggcc acggcagaga ggttgcgcgc cgccgtggag
     1261 gccgcctctt tcagcgtgcc caacaacacc acccaactgc acgtaacgat gagcgtgggt
     1321 gtggcctgct tccctatcga cgcagagacc cccaccgaca tgatacacag ggccgacatc
     1381 gcggtgtacc aggccaagct caacggccgc aactgcgtgg tgtgtggctc cgacatcccc
     1441 cagtccatcg acttcgccgc cgcgccctcc gaccgcctgt ccgctcccta ttcaggtacc
     1501 ttcgttcccc ggcccaccac caatggcaac ggcgcgcacc gcaatggggc caacggcttg
     1561 cacggttcgg ctatgccttc cgctaccctc gccgccaggc cctcaagcgc ccctgccaag
     1621 gtcccggtcg cccccgtaac cctcccggta ggcaggcccc cgctcaggcc caacaccgcg
     1681 cccatacgcg ccgaaggcac ggcacccctg atctcgcccc tgaccgcggc gctcgcaccg
     1741 ggggcggtca tttccgcggc tataacccac cctatcactc acccgataac cgagcctcaa
     1801 ctcaaggccg agaagtaccg cagccatgtg cgcgagtcgt ccccatcggt gccgagcggc
     1861 ctgcaaaaag ccgtattccc gatgttcatc gccagcgtga tactggcggg cttcgctttc
     1921 gccttcaccg ggttcttcat gcacgccgcc cccgacccta tggcgattgg cctgttcgtg
     1981 atgctggccg tgggcgcgga gctgctgcaa ctcgacctgt acggcaaggg cacggtctcc
     2041 gtgtcggtgg cggtagcctt cgccgccgcg tgcgtgaccg gcctgcctgg actcgcgctg
     2101 gtatcggccg cgatagtgct ggcccaccgc ttgcgccaga cccagacgcc tatcctggac
     2161 aagacctcgg cctacaagac cgctttcaac tgggccactc acctgctggc aggcgtgtcg
     2221 ccggtgttcg cactgcggct cctgcacatc gagttcaggc tcgacaacct gccgatgctt
     2281 atcgccccga tgctcatcgc cgcgatggct tacttcatcg tggacacggg cctgatagcc
     2341 accgttatcg gcctggtgag cggggagaac ccggtgaagg tctggcgcga ccgctaccag
     2401 tggctcgtaa gccactattt gaccctgtgc acgctcggcc tattcgtggg cctggcctat
     2461 atgaccagcg gccccctggg cgtggtcgtc tttaccatgc cggtgttgat gatgcgcgtg
     2521 gcgatgaagc agtacatctc ccagaccgag catagcgtgc gcgagcttca gcgcatgaac
     2581 gagcaactga cccacgccaa cgcggaggtc gtgtcggcca gccgcaccat gcagtcgctc
     2641 aacgaggaac tcttcctcac cctctccaag atcatcgacg cccgcgaccc gtacgtgggc
     2701 ggccacgcct ccaaggtggg agactatgcg gtggcaatcg cgcacgacct gggtgtggac
     2761 cctgagcggt tcgagccgct acgccaggcc ggattcctgc acgatatagg caagatcgcc
     2821 atttccgagg cggtgctgca caagcccggc aagctgactg acgaggagta cgagtacatc
     2881 aagacgcacg cggcgctggg cggtgagttc ctggagatgt gtcgcggcct gcggcacctg
     2941 gcgcctttcg tgcggcacca ccacgagcgc tgggacggca agggctaccc cgacaagctg
     3001 gaaggggaag tgatccccct ggaagcccgc atactcgctg tgtgcgacgc ggtggaagct
     3061 atggcctccg accgccccta tcgcaagggc atgtcgctcg acgaggtgat agccgaggtg
     3121 aaacgttgcg cgggcacgca gttcgacccg cacgtggctg ccgccttcgt gcgcatcgcc
     3181 gagcgcgagc gggaccatct gattgtcaac tcggccacgg aagtgcaccg
//
LOCUS       DASRWZ010000476         4963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_518998, whole genome shotgun sequence.
ACCESSION   DASRWZ010000476 DASRWZ010000000
VERSION     DASRWZ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518998"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1087
                     /locus_tag="VFH60_07440"
     CDS             242..1087
                     /locus_tag="VFH60_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET6313652.1"
                     /translation="MSHNVGVFVDVSNLFYSAKSAGVEVNYCRLLEHAAAGRDLIRAS
                     AYTGVDPENPNQRKFIDFLRANGYKVICKDIHKYEGGRIKANLDIEMAVDIMLLSENL
                     DVVVLVSGDGDFVRLIEAVQMKGVRCEVIGFGISTSNELIDIADQFTEIGALPGIFRN
                     APSGPGVHIKSMQGAGADSRSVYAYSSYSVSASYGVQPERVSGERERERLRQGGDRPH
                     QTYWDQREGRQQQLEGNGGYDFATHDEFSLPDADACADVHSIPLPRPHQGQLPTGNGS
                     GSRHE"
     gene            1080..2255
                     /locus_tag="VFH60_07445"
     CDS             1080..2255
                     /locus_tag="VFH60_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HET6313653.1"
                     /translation="MSSSGTRTSSAQNVGYAAPTTGKEFSLVAPSGAPGWDRGMSAAS
                     ASLLQSWNWGEFKKQAGWSPLRLALRRANETPSISAQVLFRSVPRLPLPVSAAYIPRG
                     PVAMPGSNVDDRAEEAFWAGVHRAAKKRGAVFLKVEPNLELGEGVTRADVDRKMQALG
                     FRPSGRLQPARTIVLDLDKSEDELLKAMKPKTRYNLRLAGRRGVVVRRAGTLDDLRAF
                     YKLLEVTGERDEFGIHTFPYYEHMWKVFGPGGDNSALVLLADHPDEGEQAEGPISGLL
                     TLKFGREAIYMYGASANRGREHMPNYLLQWAAIRWAKEQGCTVYDFWGIPDAPTEDGD
                     EGSNSVSPVNARSGMSGVYWFKKGFGGREIEYPGAYDYVYNRVGYALWMRWRGANLG"
     gene            2312..3388
                     /locus_tag="VFH60_07450"
     CDS             2312..3388
                     /locus_tag="VFH60_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HET6313654.1"
                     /translation="MRKFIVIVALLLLGATLLGSMPPLARNANGPVAAVSSAADIRGK
                     IAYTKGGAIWVYTGGKEVQITNGPADALDKRDASPAISPDGTEIAYIRFDEGFSDLYT
                     LDIDDPDDPTALTDYRPRGVDTGDLAQGNQPGYADQALWALYPAWSPDGTQIAWTHDR
                     GIEYPGLFLIDADGDNPERTSFLDHSTQAIEHVSWSPDGTQIAASNYVGTPGGTGQIW
                     VLTLETGRWLDYTSVKDGAYDPAWSPDGEWIAFVQREGTAHNIYVMRADPDDWGTGSP
                     NGTDAATVAGPTLYKLTIDGSARSPAWSPDGTKLAYIGLEDDTFDIYAADFSVAGTIP
                     SISNIERLTDNANVEAQWGLSWGK"
     gene            complement(3513..3950)
                     /locus_tag="VFH60_07455"
     CDS             complement(3513..3950)
                     /locus_tag="VFH60_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6313655.1"
                     /translation="MKVSDIMTKDPDSVTFGTSIGDVAGLMRDLDVGIIPVVKDGELR
                     GVITDRDITIRVTAAGLGPYEASVQDFLSPNVVTISPDADLDEARRLMSERQIRRLMV
                     TEDGKEDGKLIGVLSIGDIAVKDHSEDTETGEVLQDISEPTKD"
     gene            complement(4321..>4963)
                     /locus_tag="VFH60_07460"
     CDS             complement(4321..>4963)
                     /locus_tag="VFH60_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313656.1"
                     /translation="AGHFGLGAMVKAREPQVPLWSLMLATSLLDVVFLGLYAAGIEGF
                     SSATGGEVGYGELLINAPYSHSLVGALAISLVALVVTAIAWGRRNGLVIGFMVFSHWV
                     LDLLVHRPDLPVLPGNAGNLPLLGLGLWMVPWLSAFVELCLVLAGAYMYYHAAMRSAV
                     RAERADAKAGASAVAASAGGGGGGIYRQQALVSSVAMFVLLLGTLAANFLLGG"
BASE COUNT         1031 a         1612 c         1476 g          844 t
ORIGIN      
        1 aggaacatga gggtagtctc cgtaggagac tttgtccctg gtaagcccgc caattcattg
       61 gcagggcgtc ccccgccgca gcctctctca ctaaccacta accactaacc actaaccact
      121 gaccacgccc accaatttgc cgcttgcttg acacaattat caaggtttta ttgtataatc
      181 acaccatata caagcctcgc atcattgaaa ccggccatat catactcacg gagatacaca
      241 aatgtcgcac aatgtaggcg tattcgtaga cgtttcgaac ctcttttact cggccaagtc
      301 ggccggggtg gaagtcaatt actgcaggtt gctggagcac gcagcggcgg gacgcgacct
      361 catcagggct tccgcgtaca cgggcgtcga tcccgaaaac cccaaccagc gcaagttcat
      421 cgatttcctc cgcgccaacg gctacaaggt catctgcaag gacatccaca agtacgaagg
      481 cgggcgcatc aaggccaacc tcgatataga gatggccgtt gacatcatgc tcctgtccga
      541 gaacctggat gtggtcgtgc tcgtgagcgg cgacggcgac ttcgtgcgcc tcatcgaagc
      601 cgtgcagatg aagggcgtgc gctgcgaggt aatcggcttc ggcatcagca cctccaacga
      661 gctgatagac atcgctgacc agttcaccga aattggcgct ctccccggca tcttccgcaa
      721 cgcgccttcc ggccccggcg tgcacatcaa gagcatgcag ggcgcgggtg ccgactcgcg
      781 gagcgtgtac gcatatagct cctacagcgt cagcgccagc tacggcgtgc agcccgaaag
      841 ggtctccggc gagagggagc gcgagcggct gcgccagggc ggagacaggc cccaccagac
      901 ctactgggac cagcgcgagg gccgccagca gcagttggaa ggcaacggcg gctacgactt
      961 cgccacccac gacgagttct ccctgcccga cgccgacgcc tgcgcggacg tgcactctat
     1021 cccactaccc cgcccgcacc agggccagtt gcccaccggc aacggctccg gcagcaggca
     1081 tgagtagtag cggcacccgc acatcgtccg cacagaacgt gggttacgca gccccgacaa
     1141 ccggcaaaga gttctccctc gtcgcccctt ccggcgcgcc cggctgggac cgcggcatga
     1201 gcgcggcgag tgcgagcctg ttgcaatcgt ggaactgggg cgagttcaag aaacaggcgg
     1261 gttggtcgcc gctacggctg gcgctacgcc gggctaatga aacgcccagc atctcggccc
     1321 aggtattgtt caggtcggtg ccacgcttgc cgctccctgt ctccgccgcc tatataccgc
     1381 gcggacctgt cgccatgccc ggcagcaacg tcgatgaccg agcagaagaa gctttttggg
     1441 cgggggtgca cagggcggcg aagaagcggg gggccgtctt cctcaaggta gagccgaacc
     1501 tcgagcttgg ggagggcgta accagggcag acgtggaccg caagatgcag gcgctcggct
     1561 ttcggccctc agggcgtttg cagcccgcgc gcaccatcgt gctggacctc gacaagagcg
     1621 aggacgagct gctcaaggcg atgaagccca agacccgcta caatttgcgg ctggcgggca
     1681 ggcggggcgt ggtcgtgcgc cgggccggaa ctttggacga tcttcgggcg ttttataaat
     1741 tgttggaggt aacgggcgag cgcgacgagt ttggcatcca caccttcccc tactacgagc
     1801 acatgtggaa ggtgttcggc cccggcggcg ataactcggc gctggtcctg ctggccgacc
     1861 acccggacga gggggagcag gcggaggggc ctatatcggg cctgctgacg ctcaagttcg
     1921 ggcgcgaagc tatctacatg tacggggctt cggccaatcg cgggcgggag cacatgccca
     1981 actacctgct acagtgggca gccatccgct gggcaaagga gcagggctgc accgtgtacg
     2041 acttctgggg cataccggat gctcccaccg aggatggcga cgagggcagc aactcggttt
     2101 ccccagtaaa tgcacgttcc gggatgagtg gtgtatactg gttcaagaaa ggcttcgggg
     2161 gcagagaaat agagtatccg ggcgcgtatg attacgtgta taaccgcgta gggtacgcac
     2221 tctggatgcg ctggcgcgga gccaacctcg gctaggtccc ggtagtacgc gaccgcttac
     2281 taccggcata cttcttacgg acaagggctt gatgagaaaa ttcattgtta tagtagccct
     2341 gctactgttg ggcgcgacac tcctgggcag catgcctccc ctcgcacgga acgcgaacgg
     2401 ccccgtggct gccgtcagca gtgcggctga tatacgcggc aagatcgcct acactaaagg
     2461 cggggctatc tgggtgtaca cgggcggcaa ggaggtccag atcaccaatg ggcctgctga
     2521 tgcgttggac aagcgcgacg cctcccctgc tatctcaccg gacggcaccg agatcgccta
     2581 catccgcttc gatgaaggct tctccgacct ctatacgctc gacatcgacg accccgacga
     2641 ccctaccgcg ctcaccgact acaggcccag gggcgtggac acgggcgacc tcgcccaggg
     2701 caaccagccg ggttacgccg atcaggccct gtgggcgctc taccccgcat ggtcccccga
     2761 cggcacgcaa atcgcctgga cgcacgacag gggtatcgag taccccggcc tcttcctgat
     2821 agacgcggac ggcgataacc ccgaaaggac cagcttcctc gaccacagca cgcaggcgat
     2881 agagcacgta agttggtcgc ccgacggcac gcaaatagcc gccagcaact acgtgggcac
     2941 gccgggcggc accgggcaga tttgggtcct cacgctggaa acgggcaggt ggctcgatta
     3001 tacgagcgtc aaggacggcg cgtacgaccc tgcatggtcg cccgatggcg agtggatcgc
     3061 cttcgtgcag cgtgaaggca ccgcccacaa tatctacgtc atgcgcgccg accccgacga
     3121 ctgggggacc ggcagcccca acggcactga cgccgccacc gttgcaggcc caactctcta
     3181 caagctaacc atcgacggct cggcgcgctc ccccgcctgg tcgcccgatg gcactaaact
     3241 ggcctacatc ggcctcgaag acgacacctt cgacatctac gccgccgatt tcagcgtggc
     3301 aggcaccatc ccgtctatct ccaacatcga acgcctcacc gacaacgcca acgtagaagc
     3361 ccaatggggc ctgagctggg ggaagtagcg agtgccgagt gccgagtgaa gagagtaata
     3421 atcaataaag agcggctcac agggtgcata accctggagc cgcattacaa ttactcagca
     3481 cttggcactc ggcactcggt actcggtact cgttagtcct tcgtcggttc cgagatgtcc
     3541 tggagcacct cgccggtctc cgtgtcctcg ctgtggtcct tgacggcgat gtcgcctatg
     3601 gagagcacgc ctatcagttt gccgtcctct ttgccgtcct cggtgaccat gaggcggcga
     3661 atctgcctct cggacatgag gcgtcgggcc tcgtcaaggt cagcgtctgg ggaaatcgtc
     3721 accacgttag gagagaggaa gtcctgaacg gaggcttcgt aggggccaag gccagccgcc
     3781 gtcacgcgga tagtgatatc tctgtcggta ataacgcccc gcagttcgcc gtctttcacc
     3841 accgggatga tgcccacgtc aaggtcgcgc atcagtcctg ccacgtcccc tatgctcgtg
     3901 ccaaaggtga ccgaatcggg gtctttggtc attatgtcgc taactttcat gctaactcct
     3961 ttgagtgtag ggcacgtaca gccacgccca atcttacgct tgcgtataac gccgcatcag
     4021 cagcgcaaca atactcaggt gcacaacccg tgccagcgac tgtacagagg ctacgtgcaa
     4081 agtgccgctc gtgcggtacc aagcatccct ctgtcattct gagcgtagcg aagaatctaa
     4141 agaaggtagt agagggaggc tgttgaacga tacgtactat gatgctcacg ttcaacgcag
     4201 aggcaggagc cgcatctgag attcttcgct acgctcagaa tgacaggggc cacctttggt
     4261 tgctcgcgtg agacacttta cggcgatgcc ccggtttgcc tctgcttgac tgcactccgg
     4321 ctagccccca agcaggaagt tagccgccag cgtacccagc aacagcacga acatggcgac
     4381 cgagcttacc agcgcctgct gcctgtagat gcctccgccg cccccaccgg cgcttgcagc
     4441 cacggcagac gcgccagcct tggcatcggc ccgctcggcc cgcacagcgc tgcgcatggc
     4501 ggcgtggtag tacatataag cccccgccag caccaggcac aactcgacaa acgcgctcag
     4561 ccagggcacc atccacagac cgaggcccag cagaggcagg ttgcccgcgt tgcccggcag
     4621 caccggcaaa tcggggcggt gcaccagcag gtcgagcacc cagtgcgaaa agaccatgaa
     4681 gccgataacc aggccgttgc gccgccccca ggctatcgcc gtcaccacca gggccaccag
     4741 gctgatagcc agcgccccga ccagtgaatg cgagtaaggg gcatttatga gcagctcgcc
     4801 gtaccccacc tcgccgcccg tggccgagct aaagccctcg atgcccgcgg cgtacagccc
     4861 caggaacacc acgtcgagca ggctcgtcgc cagcataagc gaccagagcg gcacctgagg
     4921 ctcccgcgcc ttcaccatag cccccagccc aaaatgcccc gct
//
LOCUS       DASRWZ010000477         5293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_519084, whole genome shotgun sequence.
ACCESSION   DASRWZ010000477 DASRWZ010000000
VERSION     DASRWZ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519084"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFH60_07465"
     CDS             complement(<1..511)
                     /locus_tag="VFH60_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET6313657.1"
                     /translation="MRDLDYARGRAGFLSGEGSASRNFLYSAIAWLTIADFVGLIAAT
                     QMISPDFLAGIPFLTFGRLRQMHTNGVLFMWLSMAQLGAFFYIVPRLTGTKLYSEVLG
                     NITMILWNMVGIAGYLTLANGMTQAREYAELIWPLDVMVMIALLLAGYNIFRTIFLRK
                     EKKLFVSLWY"
     gene            complement(670..1560)
                     /locus_tag="VFH60_07470"
     CDS             complement(670..1560)
                     /locus_tag="VFH60_07470"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HET6313658.1"
                     /translation="MAFLASLYSMNERSFRLTPRGRALGIVGLLIGVGLAFGLFNVPL
                     ASSAANSVDITKIEEAVPTDPRSPLWERAKEAEIALSAQQIYQPGGGTTRFVRVRVLE
                     DGTNIGFRVSWEDDTRNDLTGNTPSDAAAIQLPMEPAHIPYQCMGQSTSRVNIWQWKA
                     ALEREALDNMGALPQEGAGVRNLASNGICKAAETPGIEPQVYSYHDGSMWHVIFMRAL
                     SKGDEDSAPLAPGINTAVAFAVWNGARGETRGMKAVSTWNTLLHGAPESGGAGGLVTL
                     GIVIAVSAGVIAYSMRRLAQ"
     gene            complement(1587..2468)
                     /locus_tag="VFH60_07475"
     CDS             complement(1587..2468)
                     /locus_tag="VFH60_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6313659.1"
                     /translation="MRDKVILGVTLSIVLLLTLVIYGLIDANRSVTVQASKSEDDARR
                     GKHHYAQYCIQCHGPLGEGCIGPALNRATWRPKLGDGTNNPNWDPATYDLMKRTIVRG
                     RPSNQPGQQMPAWSVQEGGPLNDAEIEQLITFIQIGDWGTTLEEATSATGLGEELPAF
                     SGFDDKARVAEAREVMLEKGCLNCHRMGKAGGVVGAELTDVGSRRTADWLRKWIKDPK
                     SVPAEERGPNLWLVAPTATLPLPSGAAGQPRGTVEPTATPNIYPMNRTYMPTIPMTDE
                     ELDLLVDYLSHARVTTP"
     gene            complement(2551..3135)
                     /locus_tag="VFH60_07480"
     CDS             complement(2551..3135)
                     /locus_tag="VFH60_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HET6313660.1"
                     /translation="MAIADRTGRGETLTPRTGADAPGRPEDTAGGRVAPTRRRDLLKW
                     GLFGSLLLMLGGFGQAFLMFFYPKKVGVFGSRVTVEGTVADYPVDTVTAVREGKFYLS
                     HVPEGLLALYWKCKHLGCTVPWKQDEEFQGNQGVFHCPCHGSIYLRNGQNVAGPAPAP
                     LDIMKLELTGNKIVVDTGKITPRVQALPSDAVKV"
     gene            complement(3205..4074)
                     /locus_tag="VFH60_07485"
     CDS             complement(3205..4074)
                     /locus_tag="VFH60_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome C reductase"
                     /protein_id="HET6313661.1"
                     /translation="MATTGDNLTTREQNVTVQKGSKVLTPPARNRPTTPADQEVDTVQ
                     VWPHLVVIEFLGAMIITINLVLLSTLFNGPLEQLANPDKTPNPSKAPWYFLNLQELLL
                     HMNPALAGVIVPTIALGLIAAIPYIDRGTRGLGVWWYSDRGAKIAIFTTIYSILVTAA
                     LIAFDNIVKLRTATLDLFKPEALLGGVTTFTGGILGGPTPEAANASAQGFLVELFAGW
                     LIPTFCLLFFPALLVLLLKRVFKGIDLAEVIIGLFTGFVVVYWVLTIVGTAMRGPGMD
                     LYPPWALPAPMEG"
     gene            complement(4116..4997)
                     /locus_tag="VFH60_07490"
     CDS             complement(4116..4997)
                     /locus_tag="VFH60_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET6313662.1"
                     /translation="MARIARRIPRFPRPGDILDKLPRPIRTRVDKMAALGKRTWEDVS
                     GSIVRNGWPTSVRGRSLAVMNSVFLHLHPIRTRPDAIKMTYTFGLGGISFFLFLLLTL
                     TGVLLMFYYVPSTTLAWQTLHDIQETQPFGFLLRNLHRYAAHGMVFTVFLHMCRVFYT
                     GSFRAPRQFNWVVGVILLVLTFLLSYTGYLLPWDQLAYWAVNVGVNMVGSTPFVGDLV
                     KFLFIGGYTVDQNALIRFYTLHVVALPLALAVFLAVHFWRIRKDGFSRGLTFTSRVVE
                     HEDRRSAGARAAGQKKD"
     gene            complement(4997..5128)
                     /locus_tag="VFH60_07495"
     CDS             complement(4997..5128)
                     /locus_tag="VFH60_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313663.1"
                     /translation="MTLSVDLEPFIILPSLLFSIFLLVIIDRIGTIRGTVDDEEEEV"
BASE COUNT         1010 a         1630 c         1648 g         1005 t
ORIGIN      
        1 tgtaccacaa cgagacgaac agctttttct ctttgcggag gaaaatcgtg cggaagatgt
       61 tgtagcccgc gagcagaagc gcgatcatca ccatcacgtc gagcggccag atcagctcgg
      121 cgtactcgcg agcctgggtc atgccgttgg ctaacgtcag gtagcccgcg atgcccacca
      181 tgttccacaa gatcatggtg atgttgccga ggacttcgct gtagagcttg gtgccggtga
      241 ggcggggcac gatatagaag aacgccccca gctgggccat cgagagccac atgaagagca
      301 cgccgttggt gtgcatctgc cgcaaccggc cgaaggtgag gaagggaatg ccggcgagga
      361 agtccgggct gatcatctga gtggcggcga tgagacccac gaagtccgcg atggtcaacc
      421 aggcgatggc cgagtagagg aagttgcgcg acgcgctacc ctcccctgac aggaaccccg
      481 cgcgaccacg cgcatagtct agatcccgca cagctacggc cataacactc gatcctccct
      541 caactcctca acctgccctt atcaggcacc ctgacgtacc ccaggctaca tgctagcgct
      601 gacccaaata gctaacccca gacgtatctt acctacaccc ggcactcgga actcgatact
      661 cggttctcgt cactgcgcca ggcgtcgcat cgagtaggct atcacacctg ccgagacagc
      721 gattactatc ccaagcgtaa ccagaccgcc cgcgccgccc gactcggggg caccgtgcag
      781 cagggtattc caggtggata cagccttcat gccgcgtgtc tcgcctcttg ccccgttcca
      841 caccgcgaac gccacggccg tgttgatgcc cggtgcgagg ggcgcactgt cctcgtcacc
      901 cttcgagagg gcgcgcatga agatcacgtg ccacatgctc ccatcgtgat aggaatagac
      961 ctgcggctcg ataccgggcg tttcggctgc cttgcagatg ccgttgctcg cgaggttgcg
     1021 gacccccgcc ccctcctgcg gcagcgcgcc catattatcc aacgcctcgc gctcgagcgc
     1081 cgccttccac tgccagatat tcacgcggct ggtgctctgt cccatgcact ggtaggggat
     1141 atgggccggt tccataggca actggatggc cgcggcgtcg ctaggcgtgt tacccgtcag
     1201 atcgttgcgg gtatcgtctt cccacgacac ccggaaaccg atattcgtgc cgtcttccag
     1261 cacccgcacg cgcacgaacc tggtggtgcc gccaccgggc tggtagatct gctgcgcgct
     1321 cagggcaatc tcagcctctt tggcacgttc ccagagcggc gatcgcgggt cggtgggcac
     1381 cgcttcttct atctttgtga tgtcaaccga gttggccgcc gacgaagcaa gcggcacgtt
     1441 gaacagccca aacgcgaggc ccaccccgat caggaggccg acgatgccca gggcgcgccc
     1501 gcgcggcgtg agccggaagc tgcgttcgtt catgctgtac aggctggcga gaaaagccac
     1561 tctctgtcct ccgggcatca tgctcgttac ggcgtggtca cgcgagcgtg cgacaggtaa
     1621 tccacgagca ggtcaagctc ctcgtcggtc atgggtatgg tcggcatgta agtccggttc
     1681 atggggtaga tattgggcgt ggcggtcggt tccaccgtgc ccctgggctg cccggcagcg
     1741 cccgagggca gtggcagggt agcggtgggg gcgacgagcc acaggttggg gccacgctcc
     1801 tcggcgggca ccgacttggg gtccttgatc cacttgcgca gccagtcggc ggtgcgcctg
     1861 ctgcccacgt cggtaagctc agcgcctacc acgcccccgg ccttgcccat cctgtggcag
     1921 ttgaggcagc ccttctcaag catgacttcc cgcgcctcgg ccactcgtgc tttgtcatcg
     1981 aagccactga acgcgggcaa ctcctcaccc aggccggtgg ccgaggtggc ttcctccagc
     2041 gtagtgcccc agtcgccgat ctggataaag gtaatgagtt gctctatctc cgcgtcgttg
     2101 agcgggccgc cctcctgaac ggaccatgcg ggcatctgct ggcccggctg gttggaggga
     2161 cggccgcgga cgatagtgcg cttcatgaga tcgtacgtgg ccgggtccca gttcgggttg
     2221 ttggtgccgt cccccagttt gggccgccag gtcgcgcggt tgagggcggg gccgatgcag
     2281 ccctcaccaa gggggccgtg gcactggatg cagtactggg cgtagtggtg cttgcctctg
     2341 cgggcatcgt cctcggactt gctggcctgg accgtgacgc tccggtttgc atcgatgagg
     2401 ccgtagatga cgagcgtaag tagcagtact attgagagcg tgacgccgag aatgaccttg
     2461 tcccgcatat cgcctcctgg cttggcagcc tggctgcctt tcctatcctg ggaatggtgt
     2521 tgcggtgtcg tgctgctgga gcactcgaaa ctagactttg accgcgtccg acgggagcgc
     2581 ctgaacgcga ggcgtgatct tgcctgtgtc tactactatc ttgttgcccg tcagctcgag
     2641 cttcatgata tcgagcggag cgggcgcggg acctgccacg ttctggccgt tccgcaggta
     2701 tatcgagccg tggcatgggc agtggaacac gccctggttg ccctggaact cctcgtcctg
     2761 cttccacggc accgtgcagc cgaggtgctt gcacttccag tagagcgcga gcagcccttc
     2821 cggtacgtgc gacaggtaga acttaccctc gcgcacggca gttaccgtat ccactgggta
     2881 gtcggccacg gtaccctcca ctgtgacccg gcttccgaac acgcctacct tcttcgggta
     2941 gaagaacatg aggaaggcct gcccgaaccc gcccagcatg agcaggagcg aaccgaacag
     3001 cccccacttc agcaggtcgc ggcggcgcgt gggcgcgact cgccccccgg ccgtatcctc
     3061 cggccgtccc ggtgcgtcgg ccccggtgcg gggcgtaagg gtctcgccgc gtcccgttcg
     3121 atcagcaatt gccatgttcc ttctcctatt tccaggctga gagcttcaac gatacttcta
     3181 ctcgtggtac caggccgagg agtgctatcc ttccatgggc gcaggcaagg cccagggcgg
     3241 gtacaggtcc ataccggggc cgcgcatagc ggtgcccaca atggtgagga cccagtagac
     3301 caccacgaag ccggtgaaca gcccaattat tacttccgcg agatcgatgc ccttgaacac
     3361 ccgcttcaac agcaagacca gcagcgccgg gaagaagagc aggcagaacg tcggtatcag
     3421 ccagccggca aacagttcga ccaggaagcc ttgcgctgat gcgtttgcgg cttcgggcgt
     3481 cggtccgccc aggatccccc cggtaaaggt cgtgacgccg ccgagtaaag cctccggctt
     3541 gaacaggtcc agggtggccg tccgtagctt cactatatta tcgaaagcga tgagcgccgc
     3601 cgtaacgagg atgctgtaga tagtcgtgaa gatggctatc ttagcgcccc ggtcgctgta
     3661 ccaccagacg cccagccccc tcgtccccct gtcgatgtac gggatggctg ctatcaagcc
     3721 gagagcgata gtgggtacga tcacgccggc cagggcaggg ttcatgtgca gcagcagctc
     3781 ttgcaggttg aggaagtacc agggggcctt ggaggggttg ggcgtcttgt cgggattggc
     3841 aagctgctcc agcgggccat tgaacagagt cgaaagcaag accaggttga tcgtgatgat
     3901 catcgcgccc aggaactcga tcaccaccaa gtgcggccat acctgtacgg tatcgacctc
     3961 ctgatcggcc ggagtcgtgg ggcggttgcg cgccgggggc gtcagcacct tggagccttt
     4021 ttgcacggtg acgttctgtt cgcgcgtcgt cagattgtct ccagttgttg ccatcggtct
     4081 ctcctagctc tcgaacgtga caaacagcgc cgtgtctaat ctttcttctg gcccgccgcc
     4141 ctagcgcctg ccgacctgcg gtcttcatgc tcgactacgc gggaggtgaa ggtgaggccg
     4201 cgagagaagc catccttgcg gatgcgccag aagtgcaccg cgaggaaaac ggccagggcc
     4261 agcggcaggg ccactacgtg cagtgtgtag aagcgtatga gggcgttctg gtccacagtg
     4321 taaccaccaa tgaacaagaa cttcaccagg tcgcccacaa acggagtaga accgaccata
     4381 ttcacaccca cgttcacggc ccagtaggca agctggtccc aggggagcag gtagcctgtg
     4441 tagctgagca ggaatgtgag caccaggaga atgacaccta ccacccagtt gaactgtcgc
     4501 ggggcgcgga aagagcctgt gtagaacacg cggcacatat gcaggaagac ggtgaacacc
     4561 atgccgtgcg ccgcgtacct gtgcaggttg cgcaggagga agccgaaggg ctgggtctcc
     4621 tggatgtcgt gcagagtctg ccaggccaat gtggttgacg gtacgtagta gaacatcagc
     4681 agcacgcccg tgagggtgag cagcaagaac aggaagaacg agatgccccc caggccgaac
     4741 gtgtaggtca tcttgatcgc atcgggccgg gtgcgtatcg ggtgcaggtg gaggaagacg
     4801 ctgttcataa cggccagcga ccgcccgcgc accgaggtgg gccagccgtt gcgcacgata
     4861 gagccgctaa cgtcttccca ggtgcgcttg ccgagggcag ccattttgtc caccctggtg
     4921 cggatggggc gcggcagctt gtctaagatg tcccctggcc tggggaagcg agggattctg
     4981 cgggcgatgc gtgccattag acttcctcct cctcgtcgtc aaccgtgccc cggatggtgc
     5041 ctatcctgtc aatgatgacc aggaggaaga tcgagaagag cagcgaaggc aaaatgatga
     5101 acggttccag gtccacgcta agggtcatgc tttctcctcc tgagcaccgt gaatcgatct
     5161 ctccgtggga gtcactgtat tatatatgac gacctgtgcg ctgcctagac gtttaggcac
     5221 catttcccgg tgaataaagc ttaaagtttc atataaaaac gccccaatag agcatcaggg
     5281 ctgcaaattg acc
//
LOCUS       DASRWZ010000478         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1204548, whole genome shotgun sequence.
ACCESSION   DASRWZ010000478 DASRWZ010000000
VERSION     DASRWZ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204548"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFH60_07500"
     CDS             <1..204
                     /locus_tag="VFH60_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6313664.1"
                     /translation="PFDQHGFTYLVKKHYMEAGRPFRASQPRDLLRQLMGIARYRNVP
                     PEMTPQLVDAACSTYFVNSKPKH"
     gene            240..539
                     /locus_tag="VFH60_07505"
     CDS             240..539
                     /locus_tag="VFH60_07505"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313665.1"
                     /translation="MYERIDEKGKVFTPRIRTASVEVDIVTMHGIVHGFVHVKPDQRI
                     KDELNHQAERFLAVTGATLRGHLDASPREVGFVAVNKSHIITVVPVNEPRWNQEE"
     gene            740..2200
                     /locus_tag="VFH60_07510"
     CDS             740..2200
                     /locus_tag="VFH60_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET6313666.1"
                     /translation="MPSVGMVTPLPGMQPQGGPGVAPGMPQGGPTNHLNTAPTEHLAT
                     GQLAAAAPAGNMWTPVANSVGMQAPPQLVMALAQRVLSGLPQNVLIERKPEIEQMLAG
                     RFMAALSQVPGTLPPGGQEVLFKAVMDEILGYGPLEPLLEDPSISEVMINGPKQVYIE
                     RKGQLIESGVTFLDEDHVMRVAQRIVAPLGRRLDRKWPMVDARLPDGSRVNIIGPPCA
                     IGGTTITIRKFFKTPLTVQNLVEFGTMSPQMADFLRACVVGRLNIVVAGGTGSGKTTL
                     LNVLSSFIPDGERIVTIEDSAELRLNQRHVVPLEARPADVDGGGAVTIRHLVTNALRM
                     RPERIVVGECRSGEALDMLQAMNTGHDGSLTTLHANTPRDALSRLETMVLMSGMDLPI
                     RVIREQVASAVDLIVQQARLRDGSRKVTYVTEVQGMEGDTIVLQDIFRFREMGDDEHG
                     KVRGQLLPTGLSPKFISRLEAHGQKVDRAMFSSGSY"
BASE COUNT          483 a          756 c          795 g          425 t
ORIGIN      
        1 ccattcgacc agcacggctt cacgtacctg gtgaagaagc actacatgga ggcggggcgg
       61 cccttccgcg cctcgcagcc ccgcgacctg ctgcgccagc tgatgggcat cgcgcgctac
      121 cgcaacgtgc ccccggagat gacgccccaa ttggtggacg ccgcgtgcag cacctatttt
      181 gtgaactcta aacccaagca ctagcggcga actgacacaa cgtagtagga cgaacaacaa
      241 tgtatgaaag aatagacgaa aagggcaagg tattcacccc caggatccgc accgcgtcgg
      301 tggaggtcga tattgtgacc atgcacggta tcgtgcacgg cttcgtgcac gtcaagcccg
      361 accagcggat caaggacgag ctaaaccacc aggccgagag gttcctcgcc gtgacggggg
      421 ctaccctgcg cgggcatctc gacgcctcgc cccgcgaagt cggtttcgtg gccgtcaaca
      481 aaagccacat tatcaccgtc gtgcccgtca acgagcctcg ctggaaccag gaagagtgac
      541 gggtaattct taattcttat tggttaattc ttaattgccc gggcgagaat tcgcgcgttt
      601 acacaggatc gaaggaagta gcatgtcacc gttacagcca ttgaacgcta catatacgca
      661 gacagacgcg cagggcgctg ggggtgtgcc gcatatgtct cagcagccgg ggggcccatt
      721 tccaggaagt gggcagggca tgccttcggt gggtatggtc acgccgttgc ctgggatgca
      781 gccgcagggg ggacctgggg tcgcgcccgg tatgccccag ggcgggccga ccaaccacct
      841 caacaccgcg cccacggagc acctggccac cggacaactg gcggcagccg ccccggcggg
      901 caatatgtgg acccctgtag cgaacagtgt cggcatgcaa gccccgccgc agctggtgat
      961 ggcgctagct cagcgcgtgc tctccgggct gccgcaaaac gtgctgatcg agcgcaagcc
     1021 cgaaatcgag cagatgctgg cggggcgctt catggcggcc ctctcgcagg tgccgggcac
     1081 gttgccgccg ggcggtcagg aagtgctgtt caaggcggtg atggacgaaa tcctcggcta
     1141 cggcccgctg gagccgctgc tggaggaccc ctccatctcg gaagtcatga tcaacggccc
     1201 caagcaggtc tatatagagc gcaagggcca gctgattgag agcggcgtga ctttcctgga
     1261 cgaggaccac gtgatgcggg tggctcagcg catcgtggcc cccctcggca ggcggctcga
     1321 ccgcaagtgg ccgatggtgg acgcccggct gccggacggc tcccgcgtga acatcatcgg
     1381 gccgccctgc gcgataggcg gcaccacgat cactatccgc aagttcttca agacgcccct
     1441 tacggtgcag aacctggtgg agttcggcac gatgtctccg cagatggccg atttcctgcg
     1501 ggcgtgcgtg gtgggcaggt tgaatattgt ggtggccggg ggcaccggct ccggcaagac
     1561 taccctgctc aacgtgctct cttcgttcat accggacggc gagcgcatcg tcacaatcga
     1621 ggactcggcc gagcttcgcc tcaaccagcg ccacgttgtg ccccttgagg cgcgcccggc
     1681 cgacgtggac ggagggggcg cggtgacgat acggcacctg gtcaccaacg ccctgcgcat
     1741 gcggcccgag cgcatcgtgg tcggtgagtg ccgcagtggg gaggcgctgg acatgctcca
     1801 ggcgatgaac acgggccacg acggctcgct caccacgctg cacgcgaata ccccacgcga
     1861 cgcgctatcc aggctcgaaa cgatggtgct gatgtcgggc atggacctgc cgatacgggt
     1921 catacgcgag caggtcgcct cggcggtgga cctcatcgtg caacaggcgc gcttgcggga
     1981 cggctcgcgg aaggtgactt acgtcactga agtgcagggc atggaaggcg acacgattgt
     2041 gctgcaagac atcttccgct tccgcgagat gggcgatgac gagcacggca aggtcagggg
     2101 ccaactgctg cccaccggcc tgtcacccaa gttcatcagc cgcctggaag cgcacgggca
     2161 gaaggtagac cgggcaatgt tctcctcagg gtcgtactga cgagtgccga gtaatgagga
     2221 ctgaggactg aggactgagg actgagtaac ggggtgaagg tctttgggac gcgaagatgg
     2281 ctctctgctg gtgtggcggg gccatcttcc cttttgtcat cctgagcgtt gaagactctc
     2341 gcctacccac ctgatccttc gctacgctca ggatgacaaa agggaacatg gcccgactgg
     2401 accggcgtgg ctattttcac gttccacctc tagcaagccg cctcactttt gagacctgt
//
LOCUS       DASRWZ010000479         1991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_587986, whole genome shotgun sequence.
ACCESSION   DASRWZ010000479 DASRWZ010000000
VERSION     DASRWZ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587986"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            514..1425
                     /locus_tag="VFH60_07515"
     CDS             514..1425
                     /locus_tag="VFH60_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313667.1"
                     /translation="MQQNWVPMMRRRVGVFLTTGALLVGTAFAIGCVGQNEDGTYGST
                     LAQATAQPTGTLTVLPAVTVGATGTATGTTSVATSATGTITPAVPVSATATVVEGTPT
                     PRPTPTGEPPDPESRLNIYQFYQTNNILVRRDSVQLDGSGADEVLYTLTGPDPVITGD
                     FRSNINVLTYDATYREWTATWSSEPISGTATPLLSVAQSEIGGLNGRDLLRTGTPILM
                     LRTTTADNRAHLHLYRWDAAGKKGDPLRMVPTGGGAEQNAAFSADLDLNVADLDDDGI
                     YEVVADNVAGVQVWKWDGQKFVPEVAR"
     gene            1425..>1991
                     /locus_tag="VFH60_07520"
     CDS             1425..>1991
                     /locus_tag="VFH60_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313668.1"
                     /translation="MSQRNPQRRASNPVKPGRTAPTVTTQARPSGMTGTRPTARIQNP
                     KSKIQNLPIEGHRLLASVALVAMLLVTLIPSPAAITAPNPAGFITPSITGVGSIPGDV
                     GIGAGGIDPFTALFPFRSLSERSGDNLASVWPEAADVLSYDPASLRARRTLVGQMYAS
                     SSGNINTKLKDTGLETLPSPDKLQGVEQA"
BASE COUNT          403 a          688 c          584 g          316 t
ORIGIN      
        1 aatgtgtaga cgccgggtag aacctctttc ataaagcact tcctcacagc atggttgacg
       61 ttggagaagg gacgagcgtg ccgataaggc gaggcggcct acctcgtagg gtagcgggag
      121 cggccaaggg gcacgtagca agattataga tgccagggaa tagagtgtca acgcggtcgc
      181 tgtacctcgc catggtgaac aatggtatgc gctgaggtgc tgggcactcg cgctgggcgt
      241 cccacaggcg tcagtcgtga tgcgtcccta ctgtagtatc tacaccctac gctggccgca
      301 ctacgatgaa gaggcataag cgctaccgga ccagctaaac cgataagccc tccgcgtagt
      361 gttaacaacc gaaggtccct tctagcttca cttacaacta acaactgacc actcccccgc
      421 tgccttgcag ccctatagcc cgcgtggtac aattgcacga tgccctaaaa tccttaatct
      481 cccagatagg agcgagcagc ttcacttatg caaatgcagc aaaattgggt gcccatgatg
      541 cgccgcagag tgggagtctt tctcacgacg ggcgccctct tagtgggcac cgccttcgct
      601 attgggtgcg tgggacagaa cgaggacggc acctatggca gcaccctcgc ccaggccacc
      661 gcgcaaccta cgggcaccct gacggtgctc cccgccgtta cggtgggcgc taccggcacc
      721 gctaccggta caacctcagt ggcgacttcc gctaccggca ccatcacccc cgccgtgccc
      781 gtcagcgcca cggccacggt cgtagagggc acgcccacgc ccaggcccac cccgaccggg
      841 gagccacccg acccggagtc ccggctcaac atctaccagt tctaccagac caacaacatc
      901 ctggtgcggc gcgactccgt gcaactcgac ggtagcggcg cggacgaggt gctttacacc
      961 ctcaccggcc ccgacccggt catcacgggc gatttccgca gcaacatcaa cgtgctgacc
     1021 tacgacgcta cctatcgcga gtggaccgct acctggtcct cggaacccat cagcggcacg
     1081 gccacgcccc tgttgagcgt ggcgcagtcg gagattggcg ggctgaacgg gcgcgacctg
     1141 ctacgcaccg gcacgccaat cctcatgctc cgcaccacga ccgccgacaa tcgcgcgcac
     1201 ctccacctgt accgctggga cgccgctggc aagaagggcg acccgctcag gatggtgccc
     1261 accgggggcg gtgccgagca gaacgctgct ttctccgccg acctcgacct caacgtggcc
     1321 gacctggacg acgacggcat ctacgaggta gtggccgaca acgtcgcggg ggtgcaagtg
     1381 tggaagtggg atggccagaa attcgtcccg gaggtggcgc gctaatgtct caacgcaacc
     1441 cccagaggcg cgcatctaac ccggtgaagc ccggcaggac cgcgcccaca gtcaccaccc
     1501 aggccaggcc cagcggcatg acaggcacca ggcccaccgc cagaatccaa aatccaaaat
     1561 ccaagatcca aaatctccca atcgaggggc acaggctgct tgcctcggtc gcgctggtgg
     1621 cgatgctgct tgtcaccctc atcccttcgc ccgccgctat cacggctccc aacccggcgg
     1681 ggttcatcac gcccagcatc acgggcgtgg gttccatacc gggtgacgtg ggcatcggcg
     1741 cggggggcat cgacccgttt accgcgctgt tccccttcag gtcgctcagc gagcggagtg
     1801 gggacaacct ggcctcggtg tggcccgaag ccgcggacgt gctgtcgtac gacccggcca
     1861 gcctgcgcgc ccgccgcacg ctcgtggggc agatgtacgc ctctagctcg ggcaacatta
     1921 acaccaagct caaggacacg ggcctagaga cactgccttc ccccgacaag ctccagggcg
     1981 tggagcaagc c
//
LOCUS       DASRWZ010000480         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_724858, whole genome shotgun sequence.
ACCESSION   DASRWZ010000480 DASRWZ010000000
VERSION     DASRWZ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724858"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..>2364)
                     /locus_tag="VFH60_07525"
     CDS             complement(225..>2364)
                     /locus_tag="VFH60_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6313669.1"
                     /translation="GLGLLSGAAFFISHSAMLGQGLTYVSWGMDFWWHAGWVPVLASP
                     FAWYVVMLWYAGYWDDPKSALRRRQRAWLFIVSLLAFGMSAFLTFANPLPSYWQLAQR
                     NLSTPYSYNGVPMLMIAFPAYMVLCIVLSMDALRKPGPTGRLMGEVARERARPWLVAT
                     AGVLLLVSLLVTWAMVWIVLHVRETPFYNLPSNLPPETLLNTIALFDLVIATLIGVSI
                     VLLGQAVVAYEIFTGKALPRRSLLLQWWSAVVLAGGFSTLVGYSLTVATHPINSLVLA
                     TILMVVFYAVFSWRSYIERERYMDLLRPFVTGRSLYGDLLDQSGPLPVSQQLEIAGPF
                     RVLCGDVLRARVADLAALGPLSPLVGPPLVYRAPEHKDGRSTAEGQVQLPLAELTPQF
                     TSPQVLWVAVDPLRFHDANLAVPLWSERGLIGVMLLGEKRDGGLYTQEEIEIARASGE
                     RMVDTQASAEMARRLMSLQRRRLAESQVLDRQARRVLHDDVLPLLHTAMIALSSRKAR
                     VQVPGDGNASPGSAVSPTGAESAGTVPPNGRGGEQDALALLGDAHRRISDLLRDMPSG
                     AAPQVARLGLLGALRKAVEEEFGTAFNGVTWHVEPEAERQTGEVAPLAAEVLYYAARE
                     AVRNAARYGRDGDPSRPLHLRVSARTHDGLELTVEDDGVGMSASARDNGGSGQGLALH
                     STMMAVVGGSLATESVTGKYTRVTLTLPKE"
BASE COUNT          436 a          845 c          717 g          366 t
ORIGIN      
        1 tcgtcctcca gcacccaaat atcacctttc tttatgccag tacggggcca gactgcttta
       61 gtgacatagg aaaagggcta catcgaggtg gaggacgaga ttcttcgctg cgctcagaat
      121 gacaaggggg gaacctggtc ctgcaacacc tactgtggcg cactcacgca ccatccccag
      181 cgagccgggt cactcagtcc ttagtcctta gtcctcagtc cttattactc cttcggcagc
      241 gtaagtgtca cccgcgtgta cttgcccgtc acgctttccg tggcgagcga cccgcccact
      301 accgccatca tggtgctgtg tagcgccagc ccctgcccgc tgccgccatt gtctcgcgcc
      361 gaggctgaca taccgacgcc gtcatcctcg actgtgagtt ccaggccatc gtgcgttctg
      421 gcggacacgc gcaggtggag gggcctcgaa ggatcgccgt cgcggccgta gcgggcggcg
      481 ttgcgcacgg cctctcgcgc cgcgtagtac agcacctccg ccgcgagtgg cgctacctct
      541 ccggtctgcc gctcggcctc aggctccaca tgccaggtca caccgttgaa cgcggtgccg
      601 aattcctcct ccaccgcctt gcgcagggca cccagcaggc caagccgcgc cacctggggg
      661 gccgcccccg aaggcatgtc gcgcagcaag tcagagattc ggcggtgggc gtcgccaagc
      721 aaggccaggg cgtcctgttc gccgccacgt ccgttcgggg ggacggtgcc cgcgctctcc
      781 gcgccggttg ggctaacggc tgagccaggg ctagcattac cgtcacccgg cacctgcacg
      841 cgggccttgc ggctgctgag ggctatcatg gcggtgtgga gcaggggcag cacgtcgtca
      901 tggagcacgc ggcgggcctg ccggtcgagc acctggcttt cggcgaggcg gcggcgttgc
      961 agggacataa ggcggcgggc catctcggcg ctggcctgcg tgtccaccat ccgctctccc
     1021 gaggcgcggg cgatctcgat ctcctcctgc gtgtataagc ccccgtcccg cttttcgcca
     1081 agcaacatca ccccgatgag gccgcgctcg ctccacagcg gcaccgccag gttcgcgtca
     1141 tgaaaacgca gcgggtccac cgccacccac agcacctggg gagacgtaaa ctgcggcgtc
     1201 agctcggcca gcggaagctg cacctgcccc tccgccgtgg acctgccgtc cttgtgctcc
     1261 ggggcgcggt acacgagcgg cggccccacc agcggcgaca acggccctag cgccgccagg
     1321 tcggccaccc tcgcccgcaa cacgtcgccg cacaatacgc gaaacggccc cgctatctcc
     1381 aattgctggg agacgggcag cggccccgat tggtccagca gatcaccgta gaggctgcgg
     1441 cccgtcacga acggcctgag caggtccatg taccgctcgc gctcgatgta gctgcgccag
     1501 ctaaagaccg cgtaaaacac caccatcagg atggtagcca gcaccaggct gttgatcggg
     1561 tgggtcgcca ccgtgaggct gtagcccacg agcgtgctga agccccccgc gagcaccacc
     1621 gcgctccacc actgcaacag caagctcctg cgcggtaagg ccttcccggt gaagatctcg
     1681 tatgccacca ccgcctgccc gagcaatacg attgataccc cgataagggt cgcaatcacc
     1741 aggtcgaaca gcgcgatggt gttcaacagg gtctcaggcg gcaggttgga ggggaggttg
     1801 tagaagggcg tttcccgcac gtggagcact atccacacca tcgcccaggt gaccaacagg
     1861 ctgaccaaca gcagcacgcc cgccgtcgcc accagccagg gccgcgcccg ctcccgcgcc
     1921 acctctccca tgagcctgcc cgtcggaccc ggcttgcgca acgcgtccat cgacaacacg
     1981 atgcacagca ccatgtacgc cgggaaggct atcatcagca tcggcacgcc gttgtacgag
     2041 tagggagtgg agagattgcg ctgcgcgagt tgccagtacg agggcagcgg gttggcaaag
     2101 gtcaggaacg ccgacatccc gaaggccagc aggctcacga taaacagcca tgcccgctgc
     2161 ctgcgcctca acgccgactt aggatcgtcc caatagcccg cgtaccagag catcacgacg
     2221 taccaggcga aaggagacgc gagcacgggc acccacccgg catgccacca gaaatccatc
     2281 ccccagctta cgtaggtaag cccctgcccc agcatggccg agtggctgat gaagaaggcc
     2341 gccccgctca gcagccccag cccc
//
LOCUS       DASRWZ010000481         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_998841, whole genome shotgun sequence.
ACCESSION   DASRWZ010000481 DASRWZ010000000
VERSION     DASRWZ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998841"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(633..1412)
                     /gene="sdhB"
                     /locus_tag="VFH60_07530"
     CDS             complement(633..1412)
                     /gene="sdhB"
                     /locus_tag="VFH60_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HET6313670.1"
                     /translation="MSQDRPESARSVRLRVLRQEKPGDQPDANRRWEEFDVPWRPSMN
                     IISCLMAIQRNPVTADGKQTDAVVWENSCLEEVCGACTMLINGKVRQSCSALVDKLAQ
                     PITLEPLTSFPVVRDLIVDRTRLFEAFKRVQAWVPIDGTYDLGPGPRMNEEVRQKSYV
                     LSTCIACASCMEACPNFNEQSPFIGPAAINQARLHNSHPTGGMHSTARLEALLGEGGI
                     AYCGNDQNCVRVCPKGIPLTESIAEMNRQVNLYAIKTLFSK"
     gene            complement(1689..3090)
                     /gene="sdhA"
                     /locus_tag="VFH60_07535"
                     /pseudo
     CDS             complement(1689..3090)
                     /gene="sdhA"
                     /locus_tag="VFH60_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
BASE COUNT          547 a          982 c          932 g          629 t
ORIGIN      
        1 tggtgccaat gtccaacaaa ggcaggcacg ggggcctgcc cctacagggg ttttgaaata
       61 cgctctaagg tggtagtggt ctcacgcccg agaacgctgc tggacgacca gtaaagggtg
      121 ccccgcgtgg cggcgaccag cacctaccca accacgtggc tgcctgtgca gtgcgaccag
      181 tcctggcttc tacaaatcct ctacaggtgc acccttgagt accttgtgat taatacgtga
      241 cccacataat aggcacccgg gttggtctgc cggaataggc actccctctc ctagtgagtg
      301 gaggtgctac tcagcaggtg gtgtatggta aaccccgtca gggagccaag aacacatttc
      361 agagagaata cattacccct gtcgtcaaag gaagtcaaca ggcggaatta ttctagcacg
      421 gtgcttcggc cccgtcaatc gattggtttg gaggggtaaa agttgtcttg ggtggtagac
      481 tttaaaccca aaagctcgcc ctttagcccc gcacaccttt aaagatgaag ggccagactg
      541 ctgcccggcc ttcttcactg cagcctggcc caacagcaga ggggccgccg ggcgtcgtag
      601 gtcgtgggac cgcgcctatg ggggtctcac cgttacttgc tgaacagagt ctttatagcg
      661 tacaggttca cctgccggtt catctccgca atcgactcgg tgagggggat gcctttgggg
      721 cagactcgca cgcagttctg atcgttgccg caataggcta tgccgccctc ccccagcagg
      781 gcttccaggc gagcggtcga gtgcatgccg ccggtggggt ggctgttgtg caatcgggcc
      841 tggttgatgg ccgccgggcc gatgaagggc gactgctcgt tgaagttggg gcacgcctcc
      901 atgcacgagg cgcacgcaat gcacgtcgat agcacgtacg acttctgccg cacttcctcg
      961 ttcatcctgg ggccggggcc gaggtcgtat gtgccgtcaa tgggcaccca ggcctgcacc
     1021 cgcttgaacg cctcgaacag gcgcgtgcgg tccacgatga ggtcgcgtac caccgggaac
     1081 gaagtcagcg gctccagcgt aatcggctgc gccagcttgt ccaccaacgc cgagcacgac
     1141 tggcgcacct tgccgttgat gagcatggtg cacgccccgc acacctcttc caggcagcta
     1201 ttctcccaca ccacagcatc ggtctgcttg ccgtccgccg tcacggggtt gcgctgtatc
     1261 gccatgaggc acgagatgat gttcatgctc ggccgccagg gtacgtcgaa ctcctcccaa
     1321 cggcgattag catcaggctg gtcgccaggc ttctcctgcc gcagcacccg cagccgcaca
     1381 cttctcgcac tctctggcct gtcttgtgac atcagggtct ccaaattcta agttctaagg
     1441 agtaacgcgt aacgagtaat gcgtaacgcg taacacgaaa ttcgcaaaca aatggactga
     1501 gagttacgcg ctacgtgtta cgtgttacgc gtttcttctc acagccgctt atccacctgg
     1561 gccaccgtcg cccctgctgc tttcgcccca tcgccgctat ggccgttggc gcccggagtg
     1621 gcagcggcta ccgcggaggc cgcttcgggc gacctggggg acgcaccggc tgccgcaccc
     1681 tcgcctttcg atacgtcgta gcggcgcggt ctcggcttga tgtactgaat gtctacgtct
     1741 tcgtacgtga gttcaggcgc gtcggtcggg cccatgaagc gcgcaaccgt cgttttgagg
     1801 aaacgctcgt cgttgcggtc gggaaagtcg ggcttgtagt gcgcccctcg gctttcgtcc
     1861 cgctgtagcg cacccagcgt gattacgcgt gaaagcacca gcatattcca caactggcgg
     1921 gcgaacagca gcgactggtt gctccagccc gatctgtcag gtatgccgat gtcacggtac
     1981 cgctccatta actcttgcag ccgggtgtcg gtctcccgca attcgtcgtt gtaacgtacg
     2041 acggtcacgt tgtcggtcat catcgaaccc atctcaagcc agagcgagaa agggttctcc
     2101 ttacccgacg cgctgacgag ccggttgtag atgtcctgct ggcgggccac ttcgcgctgg
     2161 aaaggtccct cgcccgcgcc gtccgacggc ttgctgctgc ccttggcgaa cttgtacgcc
     2221 gaaggccccg ccctgtagag gccgccatgt atgcacgaca gcaacgagtt ggcccccagg
     2281 cggttggccc cgtggtactg gtagtccact tcgcccgagg cgaacaggcc tgggatgttg
     2341 gtcatctcgg tgtgctggtc ggtccacaag ccgcccatcg agtagtgcac gccggggaag
     2401 atgcgcatcg gcacctttgt ggggtcatcg cccacgaact tgcgatagat gtcgatcacg
     2461 ccgcctagct tcacttccag catgtggggg tcgaggtgcg agatgtcgag gtagacctgg
     2521 ttctgcccgt ccacgccgag cttctgccgc acgcacacgt cgaagatggc gcgggtagcg
     2581 atgtcgcggg gcacaaggtt gccgtaggcc gggtacatct cttcgaggaa gtaccacggc
     2641 ttgccgtcct tgtaggtcca cacgcgcccg ccctcgcccc ggatcgactc ggacatgagc
     2701 cgcagcttgt cctcgcctgg aatagccgtg gggtgcacct ggatgaactc gccgttggcg
     2761 taccaggccc cttgctggta gacggcagcc gccgcgctac cggtatttat catcgagttg
     2821 gtggacttgc cgaagatgag gcccggcccg cccgtcgcca ggattaccgc gtccgccttg
     2881 aacgcccgta tctccatcga atgcaggttc tgcgcgacga tgcccctgca cgtgccgtca
     2941 tcgtcgacta ccgccgacag gaactcccag ttctcatcct agacgacccg cccctcgccc
     3001 tcctggcggc gggcctgctc gtccagcgcg tagagcagct gctggccggt ggtcgccccc
     3061 gcgtatgccg tgcggtggtg cagcgtgccc
//
LOCUS       DASRWZ010000482         1571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_313761, whole genome shotgun sequence.
ACCESSION   DASRWZ010000482 DASRWZ010000000
VERSION     DASRWZ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313761"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1571)
                     /locus_tag="VFH60_07540"
     CDS             complement(<1..>1571)
                     /locus_tag="VFH60_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6313671.1"
                     /translation="VILAAGVPWRRTPGFPLFILDWGIASAAILVTGGSFSPFIILYY
                     SLAIGAALRVGLSRSLIIVAACALVYVGLSVTSPAAMEAFRLPILVVQVTSLVMVALT
                     AVAMKRAVEVEARRVELEERAAGQLRLLHHLTNTVLSASPDLERVMRTVAAVSSEAVQ
                     ADSGLAVMFKDGPLEPGAGTGIFNGDNLLLVSDRAPDPPHLSEHETQLLSESIRGQAP
                     ILLQDVAATHLPGCAPCFPGLERDGSVVQAVACVPFLLGGGVLGVLFVGRYARVPFGG
                     PEVNLLGAIGQQMAVAVRLARLYEMEREKAARSEERERLERDLLSMVSHELRTPLTAI
                     KTSVGALTEADAGPATEGDMTRTRLLHNIERSTDRLTNLVAELLDSARLRAGRVSLKL
                     QQLNLGEVIVDLAPQVRPLLDARGQRLALDLPMPGSGRWARLVVSGDRRRLEQVLLNL
                     LANANKYGPSGGTITVGATPRDGQVKVFVRDEGPGIEPHEQDRIFEKFYQGQGQENAA
                     EARPESLGLGLAIAR"
BASE COUNT          280 a          534 c          496 g          261 t
ORIGIN      
        1 agcgcgctat cgcaagcccc agcccgaggc tctccgggcg ggcctccgcg gcgttctctt
       61 gcccttgccc ctggtagaac ttctcgaata tcctatcctg ctcgtgcggc tcgatgcctg
      121 ggccttcatc gcgcacgaat accttaacct gcccgtcgcg tggggtcgcg ccgactgtga
      181 tggtgccgcc ggaggggccg tacttgttgg cgttggcgag caggttcagc aagacctgtt
      241 cgagccgcct cctatcgccc gacactacca gcctggccca tcgccctgag cctggcatcg
      301 gcaaatcgag cgccagccgt tgcccgcgtg cgtccagcag ggggcgcacc tggggcgcga
      361 ggtcaacaat cacttcgccc aggttgagtt gttgcaattt gagcgaaacg cgcccggcac
      421 gcagcctcgc gctgtcgaga agctctgcca cgaggttcgt caggcggtct gtgctgcgct
      481 cgatgttgtg caggaggcgg gtacgtgtca tatccccttc tgttgcggga cctgcgtccg
      541 cttctgtgag ggcacccacg ctcgtcttaa tggccgtgag gggggtgcgt agctcatggg
      601 agaccatcga gaggagatcg cgctcaaggc gctcgcgctc ttcggagcgg gccgcttttt
      661 cgcgctccat ctcgtagagg cgcgccagcc gcacggccac cgccatctgc tggccgatag
      721 cgccgagcag gtttacttcc ggtcctccga acggcacccg cgcgtagcgc cctacaaaga
      781 gcactccgag cacgccgcca cccaggagga agggcacgca cgccaccgcc tgcacgacag
      841 agccgtcgcg ctccaggccg gggaagcaag gggcacagcc gggcaggtgg gtcgcggcca
      901 catcttgcaa caggatcggg gcctgcccgc gaatagactc gctcagcagt tgcgtctcgt
      961 gctcggaaag gtggggcggg tcgggagcgc ggtccgacac cagcaggaga ttgtcaccgt
     1021 tgaatatgcc cgtcccggcc cccggctcaa gcggcccatc cttgaacatt actgccagcc
     1081 cgctatccgc ctgcacagcc tcgctggaaa ccgccgcgac tgtacgcata acgcgctcaa
     1141 ggtcgggtga ggctgaaagg acggtgttgg tgaggtggtg tagcagcctc aactgccccg
     1201 ccgcccgctc ctccaactcc acacgcctgg cttctacctc aaccgcccgc ttcatcgcca
     1261 ccgccgtgag cgcaaccatg accagcgaag tgacctgcac taccaggatg ggtaaccgga
     1321 acgcttccat agccgccggg ctggtgacgc tcagcccaac gtagaccagg gcgcacgccg
     1381 ccacgataat tagagagcgt gagaggccca cgcgaagggc cgcgcctatc gccagcgaat
     1441 agtagaggat gatgaatggg ctgaacgagc cgcccgtgac gagtatcgcc gcagatgcta
     1501 tcccccaatc cagtatgaag agggggaagc cgggagtgcg ccgccagggc acacccgccg
     1561 ccagtatcac c
//
LOCUS       DASRWZ010000483         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1205008, whole genome shotgun sequence.
ACCESSION   DASRWZ010000483 DASRWZ010000000
VERSION     DASRWZ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205008"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1947
                     /locus_tag="VFH60_07545"
     CDS             <1..1947
                     /locus_tag="VFH60_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313672.1"
                     /translation="AFILYGVVGVLTHLRLFSLLATGVGGWFGLPSPAGAPVPLDFLA
                     PLRAHFDSTPWLPWAAWLLGAGLLTFGNRPPAVETGDAQIGAVAPTSRSRRWLVPSLV
                     LAALLLLAGFGRMVLLLPQDVGLSELPYDDEGVYAGASQLFLQGIMPYRDYFFAHPPV
                     AAFSYAPAMAYHFNEWGSPTSFMMARYLSVGYSLVTLVLLFLLGTRLAGLAGGAISGL
                     LWALDGRVVEINRKVMLDGPMVLLSCAALLLYLSARPYLAGEGGAPRRSAALRLLALS
                     GVCGALSTLTKIAGVACLLAILADMAWLWATYRFSSRKGEHPRRVGAGLLYALVGALG
                     GAMLVLVPFLVLAPSQFVRDVFFFQLLRPSDGIADIPSRVADLTATLRNPLTMLLAAL
                     GFLALSLLLWLRRSTSTWWVVTVWVFFSVLLLTYSRSFYQHYYIQLAAPLCLLGAAIS
                     LLPDLWRRLGPRVATALPALRFAAPALLALLLLPLLLVQWNGIVTRREDRIFEVVGRY
                     VNNAVPPGTPVLATDEQFNLLAARPPSHNATGYLVDSYGHMISLGLGLHTRELGDLWS
                     SILAGNRNAQATPGERPDVYGVMYRPVPQADFLDRVSRAPLVVLHATGRNRLTEETQE
                     EIAERKQLAVEQSTYSIYRDAAPR"
     gene            2404..3156
                     /gene="rpsB"
                     /locus_tag="VFH60_07550"
     CDS             2404..3156
                     /gene="rpsB"
                     /locus_tag="VFH60_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET6313673.1"
                     /translation="MKALLEAGVHFGHQTKRWNPKMKTYIFTERNGIHIIDLQQTVTG
                     LQAAMGFVSDLVANGGKVLFIGTKKQAQESIREEAERGGAYFVNKRWMGGMLTNFATI
                     RNRLRYLADLEGRQERGEFADLPKAEASKLEEKVEKLNSLLGGIKDMRDIPDAVFIVD
                     THRERIAITEAIKLGIPVVAMVDTNADPDEVDYVIPSNDDAIRAVRIITAKMADACLE
                     GAQRRESMRADVDVDYTIEPGEYSFTPDDAEV"
     gene            3331..>3475
                     /gene="tsf"
                     /locus_tag="VFH60_07555"
     CDS             3331..>3475
                     /gene="tsf"
                     /locus_tag="VFH60_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752369.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET6313674.1"
                     /translation="MDISAQAVKELREKTGAGVMDCKKALQDAGGNIDKAVEALREKG
                     LAKH"
BASE COUNT          612 a         1188 c         1032 g          643 t
ORIGIN      
        1 gccttcatcc tgtacggcgt ggtaggcgtg ctgacccacc tacgcctatt ctccttgctc
       61 gccaccgggg tgggaggctg gttcgggctg ccatcgcctg ccggtgcgcc cgttcccctc
      121 gactttctag cgcccctgcg cgcccacttc gatagcacgc cctggttgcc ctgggctgca
      181 tggctcctgg gcgcgggctt gctcaccttc gggaacagac cgcctgctgt agaaaccggc
      241 gacgcgcaga tcggggccgt ggccccaacg agccgctccc ggcgatggct cgtgccgtcc
      301 ctcgtgttgg cggcgctgct gctgctggcg ggcttcgggc gtatggtgct gctgttgccg
      361 caggacgtgg gtctctccga actgccctac gacgacgagg gagtgtacgc cggggcgtcg
      421 caactcttcc tccagggcat catgccctac cgcgactact tcttcgccca ccctcccgtc
      481 gccgcgttct cctacgcgcc tgctatggcc taccacttca acgaatgggg cagccctacc
      541 tccttcatga tggcccgcta cctctccgtc ggctacagcc tcgtcacgct ggtgctgctg
      601 ttccttttgg gcacccggct ggcgggtctt gcaggcggcg ccatatccgg cctgctctgg
      661 gcgctcgacg ggcgcgtggt cgagatcaac cgcaaggtga tgctggacgg gcctatggtg
      721 ctcctctcct gcgcggccct gctcctctac ttatcggcgc gtccctacct ggcgggcgaa
      781 ggtggtgctc ccaggagaag cgccgccctc cggctgttgg ccctctcagg cgtatgcggt
      841 gcgctcagca cgctgaccaa aatagcgggt gtggcctgcc tgctcgccat cctggccgac
      901 atggcctggc tttgggcgac atatcgcttt agctcgcgca aaggcgaaca cccgcgcagg
      961 gtaggtgccg gtctcctgta cgcgctggtg ggtgccctgg gtggggccat gctcgtgctg
     1021 gtgcccttcc tcgtgctcgc cccctcccag ttcgtgcgcg acgtcttctt cttccaactg
     1081 ctgcggccca gcgacggcat tgccgacata ccctcccgcg ttgccgacct caccgccacc
     1141 ctgcgcaacc ccctgacgat gctgcttgcc gccctgggct tcctggcgct ctcccttttg
     1201 ctgtggctgc ggcgctccac aagcacgtgg tgggtggtca cggtctgggt gttcttcagc
     1261 gtgctgctgc tcacctacag ccgctccttc taccagcact actacataca gcttgccgcg
     1321 cccctctgtc tcctgggtgc cgcgattagc ctgctgcccg acctgtggcg tcgcctcggt
     1381 ccccgcgtgg ctaccgccct tcccgccttg cgattcgccg cgcccgcgct actggctctc
     1441 ttgctcttac cgctgctcct ggtgcagtgg aacggcatcg taaccaggcg cgaggaccgt
     1501 atctttgagg tcgtggggcg ctacgtcaac aatgccgtgc cccccggcac gcccgtgctc
     1561 gctaccgacg agcagttcaa cctcctagcc gcccgccccc ccagccacaa cgccaccggc
     1621 tacctggtgg acagctacgg ccacatgatc tccctcggcc tggggttgca cacccgcgaa
     1681 ctgggagacc tctggtcctc catcttggcc ggcaatcgaa acgcacaagc cacccccgga
     1741 gaacgccccg acgtgtacgg cgtcatgtac cgccccgttc cccaggccga cttcctcgac
     1801 agggtctcgc gtgcgcccct cgtggtgctt catgccacag gcaggaaccg cctcaccgaa
     1861 gagactcagg aggagatagc cgagcgcaaa cagttggccg tcgagcagag cacctactcg
     1921 atttaccgcg acgccgcgcc cagatagccc ctaaacgaac ttgtctcaca ttgccgtctt
     1981 atgatataat tgccgttggc gttccgaaag gctagcctca aaagagcgct gaatagttgg
     2041 ggtgttgcgt gcccctcagt acatatcaag cacgtcacct gaccagccgg gatgtgcgcg
     2101 ccggccgcta tgaaacgcaa acattggtag cggctcgcgt gcccggcgcg aaagacggtg
     2161 gcggacgttt aacacagacg gaggattctt ttggcgacga ccgatacaga catggaccag
     2221 gtggcagccc ccgccgaggg ggatacaatc gataccccca tagcccccgc ccccgtacag
     2281 gaagcgcctg tccaggcacc ggcaccccag ccccagcccc aggcacccac ccgcggctat
     2341 gccggcggta acggctccga cggctccagc ggctccggcg gccagggtgg cagcggtatc
     2401 aacatgaagg ccctccttga ggcgggcgtg cacttcgggc accagaccaa gcgctggaac
     2461 cccaagatga agacctacat cttcaccgag cgcaacggca tccacatcat cgacctgcaa
     2521 cagaccgtca ccggcttgca agccgcgatg ggtttcgtaa gcgacctcgt agctaacggc
     2581 ggtaaagtgc tgttcatcgg caccaagaag caggcccagg aatcgatccg tgaagaggcc
     2641 gagcgcgggg gcgcgtactt cgtaaacaag cgctggatgg gcggcatgct caccaacttc
     2701 gccacgatcc gcaaccgctt gcgctacctg gccgaccttg aaggccgcca ggaacggggc
     2761 gagttcgctg acctgcccaa ggcagaggcc tccaagctcg aagagaaggt cgagaagctg
     2821 aacagcctcc tgggcggcat caaggacatg cgtgacatcc ccgatgcggt gttcatcgtg
     2881 gatacccacc gcgagcgcat tgccatcacc gaggcgatca agctcggtat ccccgtcgtc
     2941 gctatggtcg ataccaacgc cgaccccgac gaggtagact acgtgatccc ctccaacgac
     3001 gatgccatcc gtgccgtgcg catcatcacc gccaagatgg ccgacgcctg cttggagggt
     3061 gcccagcgcc gcgagagcat gcgcgccgac gtggacgttg actacaccat agagccgggc
     3121 gagtacagct tcaccccgga cgacgccgag gtataagaag caccgggtat cagctaccga
     3181 gtagccaggc aggcaacaag catgggggcg gctgggtaag cctggccccc tttttgcgag
     3241 ttttgagtta tgagttttga gtgttgagtt gctcagaacg ataattaact caaagctcat
     3301 aactcaaaac tcataacttt ggaggtaagt gtggacattt cagcacaggc agtaaaggaa
     3361 ctgcgcgaga agacaggcgc cggagtgatg gactgcaaga aggctttgca ggacgctggc
     3421 ggcaacatcg acaaggcggt ggaagcctta cgagagaaag gcctggccaa gcaca
//
LOCUS       DASRWZ010000484         7202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_793710, whole genome shotgun sequence.
ACCESSION   DASRWZ010000484 DASRWZ010000000
VERSION     DASRWZ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793710"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..318
                     /locus_tag="VFH60_07560"
     CDS             97..318
                     /locus_tag="VFH60_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313675.1"
                     /translation="MADQDRSTQDQGLSDAELQDQEINQLPDREAMSLIDANVAAPVN
                     LAAALNVLSDGSVAYANAEQTVDIDQSTG"
     gene            383..634
                     /locus_tag="VFH60_07565"
     CDS             383..634
                     /locus_tag="VFH60_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313676.1"
                     /translation="MADQKNSQNDGLSAEELQAQDVNELPDREAMSLINANIAVPVNA
                     AVAANVLSDGAIAYADADQYADIDQSTGTPLPGLTGTGG"
     gene            800..3658
                     /locus_tag="VFH60_07570"
     CDS             800..3658
                     /locus_tag="VFH60_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313677.1"
                     /translation="MRNTPTPGGGHPTPADDTGANTSAFPGGARPSNGPQQPTHGNGR
                     AVAPTPQPAPASQEAYPVVPGAVKGASPDAPTQAQAVVNGQPAGVPNTGGNGVTSANG
                     QGAGAQAAPQASQGQQQAQTEQQPGQQAGQQVQVEVPEKPKLADDINLKGEMQESAFE
                     EPPWLLERDGGYIQVPKLLYHVAEQCTGDQTHEQMAEAVGQKLGRKVTADNIKTLVAK
                     LIPMGIVDVPGLPKEARKRGSAAGALSVNAKMLRIPPHVFSPLSRVLQVFYLKPVLLL
                     TLLVTFAAEAWMFFVHGLGGGIHDALYTPGLLIAVFALVIVATAFHEFGHASALQKGG
                     GQVGEMGAGIYIVYPAFYTDVTDNYRLSRWARVRTDLGGIYFSLIFSAALMALYVATD
                     SAFLLMVVLFINFEIFHNMLPFVRLDGYWTLADITGIPDFFSMLAPYCRTHKPFKWLP
                     LPDGRKCPNLKPWARWVFALYIIITVPLLAFLLVMMVMGVPRLLATFWDAAWQQVGNI
                     GKAWDTGDFLGVVAAGGQMLLLALPTFALLFTLFNLGKRLVTGVWNWGKKSPRNGVIA
                     GLGSVAAALLLLFLWSPSLLLSLFVRGVPERGPLQEIVQFRPIGQQERFAVGDILTGQ
                     VVTTTRKSGLALFGLDFGSPPTPVTATTTPTITATTTITAGNVLTVTATPRSRGSATA
                     PAGTGTPRTSTPTVAVTATATLTATLGTLTPTVTPTSTPGSPVASVAPSTTGTVALPT
                     SQGTVQPTATRAPGGTGTATTAPLGGTLTPATGSTAQPTGTSGAGLPTTTSAASTPTT
                     PPAGATAPASSSTPTAGSGTGSTPVAPAPTSATVPTATPAVAPPAPTNTPVPAGTTVV
                     QPTSAPIDDTPVVAPTSTPIPTSTPTPTPTSTPTFTPTPTPTSTPVPVEPTAVPEVAA
                     TATPSPSAAQDTPVTDTAPTATSVPVTDPAPPTATTIP"
     gene            3710..5185
                     /locus_tag="VFH60_07575"
     CDS             3710..5185
                     /locus_tag="VFH60_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313678.1"
                     /translation="MRVIVALASRMNRVALVALFVLVLGALVVGGTTLVLSDQASTVT
                     TDAASTTEKQSLPTPTAIGEVGGVTPGATTGTVTSDTSATPDGSPTAEGTGTPATATA
                     GQPTSTPDAAAAPNFNPNGGGGNNVVRANNNVNGRLQVRGNLEMNRIPAPKVGPVNFA
                     EAKSSCTDCQTFATALQVNLYERGAHQVTPQNAAVALNINCTRCITVARAIQYVIPVD
                     DLKQTPKEVDDLFKALDRELRDINQSAKTAQEANQRINGVIAQFRGLSQYLRDAHQET
                     TAGGTPTPSATATATVVGTVAATAAGTGTAATGSTPGTAVPTNTQAVVATATATAAAT
                     AATATAVPGGQATQPPTPTQVPQTSPTTGNAAPTNTPGSQGNTPVPTSTPNAPPTATP
                     VPPEPTPTPVPPTPVPPTPVPPTPVPPTPTAAPPTATPAPPEPTATLAPPTQAPATPT
                     PLPPTSAPTEAAPTSTVQSDSPTTPQPTTGSEDAPTAQPTP"
     gene            complement(5393..6958)
                     /gene="malQ"
                     /locus_tag="VFH60_07580"
     CDS             complement(5393..6958)
                     /gene="malQ"
                     /locus_tag="VFH60_07580"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET6313679.1"
                     /translation="MNPDSSTASPVLSRRSSGILLHPSSLPGPWGIGDLGPEANAFVD
                     FLHATGQRLWEVLPLGPTGYGDSPYQSLSAFAGNPNLVSPERVVEDGLLSPEDLAGIE
                     PFPEGVVDFGAIIPLKRRLLQRAYERFRAGARPELIEPLRDFRAEHQGWLEDFALFAA
                     LKNAHGGVQWLAWEEELACRDARALEHAREELKEEIEYQVFAQFVFFRQWLSLKRYAN
                     QRDVQIVGDIPIFVGHDSADVWVHQDIFYLDETGRMTAVAGAPPDFHISTGQLWGNPL
                     YRWDVLKERGYEWWVERFRMALTQADVLRLDHFRGFANFWEVAADAESAAGGKWAPGP
                     GADLFEVAARELGPLPLVAEDLGLITPDVHALRQQLGYPGMKVLIEAFYGNADNPYLP
                     HNYTHDFMVYPGTHDMQTVRGWWAVAPPAVKDNARIYMGVDGHDVAWDFIRLALASVA
                     DMAVIQMQDLLDLDNSARMNTPGTTMGNWTWRYRREQLDHVVAERLRTLTRIYGRSMP
                     PPTEATEPMPGRP"
BASE COUNT         1341 a         2504 c         2167 g         1190 t
ORIGIN      
        1 tattgaccag agcaccgggt aggagatttc ggggttacat cttaacccca ctcgtaagcc
       61 ggaatagtcg atagttgaac gttgaaggag gtagaaatgg ccgatcagga taggagcacc
      121 caggaccagg gcctcagcga cgccgagttg caggaccagg aaattaacca gcttcccgac
      181 cgcgaggcga tgtccctcat tgacgccaac gtggccgcgc cggtgaacct ggcagcggcg
      241 ctcaacgtgc tgtccgacgg ctcggtggcc tacgccaacg cggagcagac ggtcgatatc
      301 gaccagagca ccgggtagac cagagaacga gcctttcaag ctgtaactaa ccggacgaac
      361 tgagacacat aaggaggctt agatggctga ccagaagaac tctcagaacg atggactgag
      421 cgcggaagaa ctgcaagcgc aggacgtgaa cgagcttccc gaccgcgagg cgatgtccct
      481 cattaacgcc aacatcgcgg tgccggtgaa cgcggcagtg gcggccaacg tgctgtccga
      541 tggcgcgata gcctacgccg acgccgacca gtacgccgac atcgaccaga gcacaggcac
      601 cccgctgccc ggcctgaccg gcaccggggg ctgatagggc cacttgaggc catgtaatta
      661 gcttttagtg gggggctggg acggttgcat cgcggcagtt cccggcccct tcgttttccc
      721 tcacaagtac gaatctacag ggttgcaccc gatacaggct gagttcgagt gattgacaca
      781 ccggaggaag tctcagatta tgcgcaacac ccccacgccc ggcggtgggc accctacacc
      841 ggcagacgat acaggcgcta acacctctgc cttccccggc ggcgcgcgcc cctcgaacgg
      901 cccccagcag cccacgcacg gtaatggccg ggccgttgcc cctacgcccc agcctgcccc
      961 ggcttcgcag gaagcctacc ccgtcgtgcc cggcgcggtg aagggtgctt cgcccgatgc
     1021 gcccacccag gcccaggcgg ttgtaaacgg acagcctgcc ggtgtcccaa acacgggagg
     1081 taacggggta acctcggcta acgggcaggg tgcgggtgcc caggccgccc cgcaagcatc
     1141 gcaggggcaa cagcaggcgc agacggagca gcaacccggg caacaggccg ggcagcaggt
     1201 gcaggtagag gtgccggaga agcccaagtt ggccgacgac atcaacctga agggggagat
     1261 gcaggagagc gccttcgagg agcctccctg gctgctggag cgcgacggcg gctatatcca
     1321 ggtgcccaag ctgttgtacc acgtggccga gcagtgtacc ggcgaccaga cgcacgagca
     1381 gatggccgag gcggtgggcc agaagctggg ccgcaaagtc accgccgaca acataaagac
     1441 cctggtcgcc aagctgatcc cgatgggcat cgtggacgtg cctgggctgc cgaaggaggc
     1501 gcggaaacgg gggtcggcgg cgggcgcgct ctcggtgaac gccaagatgc tgcgcatccc
     1561 gccccacgtc tttagtcccc tgagccgtgt tttgcaggta ttctacctca agcccgtgct
     1621 cttgctgacg ctgctggtga cgttcgccgc cgaagcctgg atgttcttcg tgcacggctt
     1681 gggcgggggc atccacgacg cgctgtacac gcccggcctg ttgatcgcgg tgttcgcgct
     1741 ggtgatcgtg gcgaccgcct tccacgagtt cggccacgct tcggcgctgc aaaagggcgg
     1801 cggccaggtg ggcgagatgg gggcgggcat ctacatcgtg tacccggcct tctacaccga
     1861 cgttaccgac aactacaggc tgtcgcgctg ggccagggtg cgcaccgacc tgggcggcat
     1921 ctatttcagc ctcatcttct cggccgctct catggcgctc tacgtcgcga ccgactcagc
     1981 cttcctgttg atggtggtct tattcatcaa tttcgagatt ttccacaaca tgctgccgtt
     2041 cgtgaggctc gacggctact ggacgctggc cgacatcacc ggcatacccg acttcttctc
     2101 gatgctggcc ccctactgcc gcacccacaa gcccttcaag tggctgccgc tgcccgacgg
     2161 gcgcaagtgc cccaacctga agccctgggc caggtgggtg ttcgcgcttt acataatcat
     2221 caccgtgcca ctgctggcgt tcctgcttgt catgatggtc atgggcgtgc ctcgcctcct
     2281 ggcgaccttc tgggacgcgg cctggcagca ggtgggcaac atcggcaagg cctgggacac
     2341 aggcgacttc ctgggcgtgg tggcggccgg ggggcagatg ctcctgctgg cgctgcccac
     2401 tttcgccctg ctctttacgc tgttcaacct gggcaagcgc ctggtaacgg gcgtgtggaa
     2461 ctggggcaag aaatcgcccc gcaacggcgt tatcgccggc ctgggcagcg tggcggcggc
     2521 gttgctgttg ctgttcctct ggtcgccctc gctgctgctc tccctgttcg tcaggggtgt
     2581 gccggagcgc ggccccttgc aggagatcgt ccagttcagg cccataggcc agcaagagcg
     2641 gttcgcggta ggtgacatac tgacggggca ggttgtgacc acgacccgca agagcggcct
     2701 ggcgctgttc ggcctcgact tcggctcgcc cccgaccccc gtcacagcca cgacgacccc
     2761 caccattacc gctaccacta ccatcacggc cggtaacgta ctcaccgtca ccgccacgcc
     2821 acgctctcgc ggctcggcca cagcgcccgc cggtaccggc acgccccgca ctagcacccc
     2881 cactgtggct gttaccgcca cggctacgct gacggctacg cttggaactt tgacgccgac
     2941 tgtcacgccc acctcgactc ccggctcgcc cgtggcctcc gtagcgccct cgaccaccgg
     3001 cacggtagcc ttgcccacct ctcagggtac ggtccagccc acggccacaa gagcaccagg
     3061 tgggacaggc acggccacga cagccccgtt gggcggcacc ctaacacccg ccacgggcag
     3121 caccgcgcaa ccgacaggca ccagcggtgc gggtctcccg accaccacat cggcagcctc
     3181 tacgcccacg acaccaccgg ccggggccac agcgcccgca agcagtagca ctccaaccgc
     3241 gggcagcggc acgggtagca cgcccgtagc ccccgctccc acgtcggcta cggtgcctac
     3301 agccaccccc gccgtggctc cgccggcccc caccaacaca ccggtgcccg ccggcactac
     3361 tgtcgtacag cccacgtcgg caccgattga cgatacccct gtggtcgcac ctaccagcac
     3421 gcctatacct acgtctacgc ccactcctac acccaccagc acgcctactt ttacgcctac
     3481 gcctacaccc accagcacgc cggtgccggt tgagcctaca gccgtgccag aggttgcggc
     3541 caccgctacg cccagccctt cggcggcgca ggatacgccc gttaccgata ccgcgccgac
     3601 agcaacgagc gtgccggtaa ctgatcctgc cccgcccacg gctacgacaa ttccttagcc
     3661 agcttggagc aagacgagca aagcgtctaa tcctgggaga atgagagata tgagagtaat
     3721 tgtagcttta gccagcagaa tgaacagggt cgcgttggta gcgctctttg tgcttgtatt
     3781 gggcgcgctc gtcgtcggcg gcaccaccct ggtcctgagc gaccaggcga gcacggttac
     3841 gaccgatgcc gccagcacca cggagaagca gtccttacct accccgacgg ctattggaga
     3901 ggtcggcggc gttacgccgg gggccacgac gggcacagtc acatcagaca ccagcgctac
     3961 gcccgacggc agccccacgg ctgagggcac cggcacgccc gctacggcta cggcagggca
     4021 gccgacttcg acccccgacg ctgccgccgc acccaacttc aacccgaacg ggggcggcgg
     4081 gaacaacgtc gtccgggcca acaacaatgt taacggccgc ttgcaggtaa ggggcaacct
     4141 ggagatgaac cgcatacccg cccctaaggt tggcccggtg aacttcgcgg aggcgaaaag
     4201 cagttgcaca gattgccaga ccttcgccac ggcgttgcag gtcaacctct acgaacgagg
     4261 ggcgcatcag gtgaccccac agaacgcggc ggtggctttg aacatcaact gcacccgctg
     4321 tatcaccgtg gcgcgggcca tccagtacgt catcccggtg gacgacctca agcagacgcc
     4381 caaagaagtg gacgacctgt tcaaggcgct cgaccgagag ctgcgcgaca tcaatcagag
     4441 cgccaagacc gcccaggagg ccaaccagcg catcaacggg gtaatcgccc agttcagggg
     4501 gctgtcccag tacctgcgcg atgcccacca ggagactacc gccgggggca cacccactcc
     4561 ctcggccact gccacagcca cggtcgtagg gacagtagcc gccactgcgg ccggcaccgg
     4621 cacggccgcc accggcagca ccccaggcac cgccgtaccg accaacaccc aggcggtcgt
     4681 ggctaccgca accgctaccg ccgctgctac cgcggccacc gctacggctg taccgggtgg
     4741 gcaggcaacc cagccgccaa ccccaaccca ggtgcctcag accagcccca ccaccggtaa
     4801 cgcagcgccc acgaacacgc cgggcagcca gggcaatacc cccgtgccta catctacccc
     4861 caacgcgccg cccaccgcca ctccggtccc gcccgagccg acgcccacgc ccgtgccacc
     4921 caccccggtg cccccaacgc cggtcccacc tacacccgta ccacccacgc caactgccgc
     4981 gcccccgacc gcaacaccgg ccccgcctga gccgacagcc actttagcgc ctcccacaca
     5041 ggcaccggcc acgcctactc cgctaccacc caccagcgcc cccaccgagg cggcacccac
     5101 cagtaccgta caatccgact cgcccaccac accccagccc acgaccggca gtgaggatgc
     5161 gcctacagcc caacccacac cctagcctgt cccactggct gtctaaacgc caagttatta
     5221 tggtttagat aatacaaaag aggagcaggg cacgtgatgt cgtaccctgc tcctctttgt
     5281 actcacgcaa ccatccttaa actttgcgcg tcctttgcgt cctttgcgtc tttgcgtttt
     5341 cgtctcttgg cgtggtcctt ggcgtcttgg cgtcttggcg tttcaaccgt atctaaggcc
     5401 gtcccggcat tggttctgtg gcttcggtgg gtgggggcat gcttctgccg tatatcctgg
     5461 tgagggtccg cagccgctcg gctactacgt ggtccaactg ctcgcggcgg tagcgccagg
     5521 tccagttgcc catcgtggtg ccgggcgtgt tcatgcgggc gctgttgtcc agatcgagca
     5581 ggtcctgcat ttggatgact gccatatcgg ccaccgaggc gagggccagc cggatgaagt
     5641 cccacgctac gtcgtggccg tccacgccca tgtagatgcg ggcgttgtcc ttcaccgcgg
     5701 gcggggcgac ggcccaccac ccgcgcaccg tctgcatgtc gtgcgtgcct gggtagacca
     5761 tgaagtcgtg ggtgtagttg tggggcagat aggggttgtc cgcgttaccg tagaaagcct
     5821 ctatcaacac cttcatgccg ggatagccaa gctgctggcg tagcgcgtgc acgtcgggcg
     5881 taatcagccc caggtcctcg gccaccaggg ggagcgggcc tagctcccgt gctgccacct
     5941 cgaacaggtc cgctcccggc cccggcgccc acttcccacc ggccgcgctc tcggcgtcgg
     6001 cggccacctc ccagaagttg gcgaagcctc ggaagtggtc gagcctcagc acgtcggcct
     6061 gggtgagggc catgcggaag cgctccaccc accattcgta gcccctctcc ttgagcacat
     6121 cccagcggta gagcgggttg ccccatagct gcccggtgct tatgtggaag tcgggaggcg
     6181 ctcccgccac cgccgtcatc ctgcccgttt catcgaggta gaagatgtcc tggtgcaccc
     6241 atacgtcggc gctgtcgtgc cccacgaaga taggtatgtc cccgacgatt tgcacgtctc
     6301 gctggttggc gtaacgcttg aggctgagcc actggcggaa aaaaacgaac tgggcgaata
     6361 cctggtactc gatttcttcc ttcagttcct ctcgcgcgtg ttccagggcg cgggcatcgc
     6421 ggcaagcgag ttcttcctcc caggcgagcc actgcacccc gccgtgggcg ttcttcagag
     6481 cggcgaacag ggcaaagtcc tcaagccagc cctggtgttc ggccctgaag tcgcgcaacg
     6541 gctctatcag ctccggccgg gcacccgcgc ggaatcgctc gtaggcgcgt tgcagcagcc
     6601 gtcgcttcag cgggatgatg gcaccaaaat cgaccacccc ctccgggaaa ggctctatgc
     6661 ccgccaggtc ttccggcgac agcagcccat cctccaccac ccgctcaggg cttacgagat
     6721 tggggttacc cgcgaaggcc gacagcgatt ggtagggcga gtcgccgtag cccgtgggcc
     6781 caagcggcag cacctcccag agtcgctggc cggtggcgtg caggaagtcc acgaaggcgt
     6841 tggcctccgg gccgaggtcg cctatgcccc aggggccggg tagggagcta gggtgcagca
     6901 aaatgccgct ggagcgccgg gatagcaccg gcgaagcggt ggagctatca gggttcattg
     6961 accctcctgg tgttgggata ttggaagcac cacgggaata ttgtcattcc gaacgtagtg
     7021 aggaatcagg tgggtaggga agaagttgta ttggagcaag tgaatggtct tgttgctccg
     7081 gcgagatccc ttgctgcgtg gcaatagtga acgactgcca cctgattcct cactacgttc
     7141 ggaatgacaa tactaccttc tatcctgtta tatgttagtt ttgactttgc atccgcctta
     7201 tt
//
LOCUS       DASRWZ010000485         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_451159, whole genome shotgun sequence.
ACCESSION   DASRWZ010000485 DASRWZ010000000
VERSION     DASRWZ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451159"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..2294)
                     /locus_tag="VFH60_07585"
     CDS             complement(63..2294)
                     /locus_tag="VFH60_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET6313680.1"
                     /translation="MNTKTFDTTLPPSTGSDLEVVDGVLEKITYINEEHGYIIARLKV
                     PRQKQLLTIAGHLAGVNIGEGLRLEGKWVNHPQYGKQFAVSRYQTAAPGTLAALKKYL
                     GSGLLKGVGPVVAEHIVNTFGLDTLEVLDANPNRLAEVPGMGKRKAATIAEAWAERRA
                     LKDLMAFLQDQGLPVALAVRILRKYGSAAESVVKEQPYRLAAEVYGIEFAVADAIAAR
                     AGVEPEAPQRVGAGVAQVLRNAASAGHVFLPVRELATGAAKLLSLSPERVSAMLPSLV
                     EAGFLMIDTLPVDFIDAAEVAYLPEMRSHELSVLESLHSLRHARKDRLALFKNTDWDS
                     AWRFLDARETVQLTSEQQFAIQSALTERVVAITGGPGTGKTTTLRGIVRLVSAKQGNV
                     VLAAPTGRAAKRLAEATGANACTIHRLLELRPGGSYEVRAPLTADLVIIDEASMMDLP
                     LAEALFKAVPAGAHLLLVGDADQLPPVGAGAVFRDIIGSGAVQTITLQTIFRQPEGSA
                     IVSNAHRINQGNMPVVGSGVKDFFFLSEPNPAECAKLVVDLATRRLPQHYGFDPMEDI
                     QVLAPIYGGVCGIEALNANLQAALNPPAATKDERRFGNRIFRVGDKVMQVVNDYERNV
                     SNGELGRIIRIDLEEQTVLVSFDSEQSVSYAFQELDELTHAYAISVHKAQGSEYPAVV
                     MPVLPQHGRLLQRNLFYTGVSRARSLVVLAGSHDALRRGVANDTSAQRYSGLVERLKQ
                     AAT"
     gene            complement(2407..2883)
                     /locus_tag="VFH60_07590"
     CDS             complement(2407..2883)
                     /locus_tag="VFH60_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET6313681.1"
                     /translation="MARTLNRVQLIGRLGGDPEMRITTSGMPVTTFSLATDRQWQDRE
                     GNMREETDWHTVVAWDRLAQICNEHLTKGRLVFVEGRLRYRSWESNGQKFYKTEVVAS
                     DMLILDPKGVSAMPEPASAHASQSAPQRPAVTVSRKAPTSTVAVLDGDEDPEDLPF"
BASE COUNT          540 a          937 c         1035 g          638 t
ORIGIN      
        1 agccatactt cagcctaaac agcgatgaga ctggagggcc ggcatcgaag acgctaattg
       61 catcaggtgg ccgcctgctt caacctctcc acgaggcccg aataacgctg agcggaagtg
      121 tcgttggcga ccccgcgacg gagggcgtca tgagagcctg cgagcaccac gaggctgcgg
      181 gcgcggctca cgcctgtata gaagaggttg cgctgcaaaa ggcggccatg ctgcggcaag
      241 accggcatga cgacagcagg gtactcgctg ccctgcgcct tgtggaccga tatggcgtaa
      301 gcgtgggtca gttcgtcaag ctcctggaaa gcgtatgaca cgctctgctc tgagtcgaac
      361 gagaccagca cggtctgctc ttcgaggtct atgcggatga tgcggccaag ctcgccgttg
      421 gagacgttgc gctcgtaatc gtttactacc tgcatcacct tgtcgcccac gcggaagatg
      481 cggttgccga agcggcgctc atccttcgtg gcggcgggag gattgagcgc ggcctgaaga
      541 ttcgcattga gcgcttcgat accgcacacg ccgccgtata tgggggcgag cacctgtatg
      601 tcttccatcg ggtcgaagcc gtagtgctgg ggcaggcggc gggtggcgag gtccaccact
      661 aacttggcgc actcggcggg gttaggctcg ctcaggaaga agaagtcttt cacaccgcta
      721 cccactaccg gcatgttgcc ctggttgatg cggtgggcgt tggagacgat ggcgctccct
      781 tccggctggc ggaatatggt ctggagcgtg atggtttgca ccgccccgct gccgatgatg
      841 tcccggaaga ccgcgcccgc acccacggga ggtagctggt cggcgtcacc caccaggagg
      901 agatgcgccc cggcaggcac cgctttgaag agcgcctccg cgagcggcag gtccatcatg
      961 gaggcttcgt ctatgatgac gaggtcggca gtcaggggag cgcgtacttc gtacgagccg
     1021 cccggcctca actcaaggag gcggtggatg gtgcaggcgt tggcccctgt ggcttcggcg
     1081 agcctcttgg cggccctgcc ggtgggcgcg gccagcacga cgttgccctg cttcgcgctc
     1141 accagccgga ctatgcccct tagagtggtc gtcttgcctg taccggggcc gcccgtaatc
     1201 gccacgacac gctcggtgag cgcggactga atggcgaact gctgctcgga cgtgagttgg
     1261 accgtctccc gcgcatccag gaagcgccag gccgaatccc agtctgtgtt cttgaagagg
     1321 gcgagcctgt ccttgcgggc gtggcgcaga gagtggagcg actccagcac cgataattcg
     1381 tgcgagcgca tttcaggcag gtaggccact tccgccgcgt caatgaaatc tacgggaagc
     1441 gtgtcaatca tcaggaagcc agcttctacc agggagggaa gcatggcaga gacgcgctcc
     1501 ggcgataggc tcagcagctt tgccgccccc gtagccaact cgcgcaccgg caagaagacg
     1561 tgcccggcgc tagcggcgtt gcggaggacc tgcgcgaccc ccgctcccac gcgctgaggg
     1621 gcttcaggct ctactcccgc gcgggcggcg attgcatccg ctacagcgaa ctcgatcccg
     1681 tatacctcgg ctgccaggcg gtatggctgc tccttgacga cgctctcggc tgcgctgccg
     1741 tacttgcgga gtatcctgac ggccagcgcg acggggaggc cctggtcctg caagaaggcc
     1801 atgaggtcct ttagcgcgcg acgctcggcc caggcttcgg cgatggtagc cgccttgcgc
     1861 ttgcccatgc cgggcacctc ggccaggcgg ttggggtttg catcgagcac ttcaagtgtg
     1921 tccaggccga aagtgttgac gatgtgctcg gccactaccg gcccgacgcc cttcagcagc
     1981 ccggagccga ggtacttctt gagggcggcg agggtgccgg gcgcggctgt ttggtagcgc
     2041 gacacggcga actgtttgcc gtactggggg tggttgaccc acttcccttc caggcgcaga
     2101 ccctcgccta tgtttacgcc cgcgaggtgc ccggctatgg tgagtaattg cttctgccgg
     2161 ggcaccttca ggcgggcgat gatgtagccg tgctcttcgt tgatgtaggt gattttttcc
     2221 aggacgccat ccactacctc caggtcgctg cctgtggagg gaggtagtgt ggtatcaaac
     2281 gtttttgtgt tcacgataag ggtgaccagt ggtcagtggt cagtggtcag tggtcagtgg
     2341 tcagtggtca gtgaccagag gctaatgcta ttttcattag cctctagccg gttggatcac
     2401 gttcaatcag aagggcaggt cctccgggtc ctcatcgcca tctaagacgg caacggtcga
     2461 agtgggggct ttgcgggaga ctgtcactgc cggcctctgc ggtgcgctct gtgatgcatg
     2521 cgcgctcgcc ggttccggca tggcagatac acccttcggg tcgagaatca gcatgtccga
     2581 tgccactact tcggtcttgt agaacttctg gccgttcgac tcccaggaac gatagcgcag
     2641 cctaccctct acaaagacca gtctaccctt tgtgaggtgt tcattgcaaa tctgcgccag
     2701 cctgtcccat gcgacgacgg tatgccagtc cgtctcctcg cgcatgttac cctcgcggtc
     2761 ttgccactgc cggtcggtcg ccagcgaaaa ggtggtaacg ggcatgcctg aggtcgtaat
     2821 gcgcatctca gggtcgccgc ccagccgtcc aatcaactgt acgcggttca atgtcctcgc
     2881 catctctcta tctcctcaaa ctaagctaat actctgcgac ggtttgtgac gtaaaacgta
     2941 aaacgtaaaa cgtaatgcgt gattggcgac tttacgtttt acgttttacg ccctacccca
     3001 ccctggcgga atcgaggcgg tctatgcggt tgggggcgcg gttgccgccg ggcatggcct
     3061 tgaggttggg gcgggctgac tgggggttgg tggcggctgg cgcggatgct accgcgcggc
     3121 ggtcctgctg gtcgggttcg gggtgcgcgg
//
LOCUS       DASRWZ010000486         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1136483, whole genome shotgun sequence.
ACCESSION   DASRWZ010000486 DASRWZ010000000
VERSION     DASRWZ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136483"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFH60_07595"
     CDS             complement(<1..372)
                     /locus_tag="VFH60_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6313682.1"
                     /translation="MNTQTRIKSREYAGEADLQPICDLLNFIDKYGDLDDGFATVADW
                     RTNLSDPETVVARDVRIWEDESGRMVGFGRLWFPRAGEEPVTDAYFFLRVHPEVRDQG
                     LEEEMLEWAEGQVRQVAQERGH"
     gene            758..1306
                     /locus_tag="VFH60_07600"
     CDS             758..1306
                     /locus_tag="VFH60_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313683.1"
                     /translation="MRKLVVVLVAAALMLSTGVALAESTFTVPFSPVAGSGVEGSAVL
                     TAAGEGTDISLDITGLTPGAPASATLHAGTCDAPSASFAALPDLTADASGRATAEGQV
                     LFRGTESVALASIADGQHVMFISQSGKQVACAWIPQVETEPASSVGMPRTGGLDLLLV
                     LSGLSVVGAIFLAGGLALRRQH"
     gene            1613..2629
                     /locus_tag="VFH60_07605"
     CDS             1613..2629
                     /locus_tag="VFH60_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6313684.1"
                     /translation="MTDIPWQVRGYRESDIPALADLIAAADRADGVHKLTTEEDLRDT
                     FETPGLEPARQVIVADGPPLPGIQKDVLLGFGRAFPSSDKARNERIYNVALRVRAEVR
                     PYALEQEIARRLVEIARQSEAALEAPPADKVRLRTYLYDTHTSARRAWEQTGLRRVRT
                     GWTMLRPLDDSIPGPQPVEGVTLRTYRRPGDNPAALQALNSAFSDNFDFQPLDEARWE
                     YAMAASYVRPDLSWVAEPLDEPGKLAGFSICWITDEENDRSGRREGWIEGIGVVPDRR
                     RQGIGRALLLNSLHSLRSAGMESALADVDSESEAPTRLFQLAGFGVRNSLSQYECYLD
                     EVKV"
     gene            complement(2804..>3149)
                     /locus_tag="VFH60_07610"
     CDS             complement(2804..>3149)
                     /locus_tag="VFH60_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313685.1"
                     /translation="AVLADTGKPHYLDRVDANEEHRGTRQSVSALLESAGFRVARVVE
                     GGFEMRFADGTALFNHTLTQLGFLGGWRGVLDPGDEESVFGEVERQLNRISAERGELR
                     MSTPMLYIEGEK"
BASE COUNT          576 a         1054 c          941 g          578 t
ORIGIN      
        1 gtgaccccgc tcctgggcga cctggcggac ctggccctcg gcccactcca gcatctcctc
       61 ctcaagcccc tggtcccgga cctccgggtg gacgcgcagg aagaagtagg cgtccgtgac
      121 cggctcttcg ccggccctgg ggaaccacaa acgcccgaat cctaccatcc tgccgctttc
      181 gtcctcccag atgcgcacgt cgcgcgccac gaccgtctcc gggtccgaca ggttggtgcg
      241 ccagtccgcc actgtggcga agccgtcgtc caggtcgccg tacttgtcga tgaagtttag
      301 caaatcgcaa atcggttgga ggtccgcctc gcccgcgtac tcacgggact tgatgcgcgt
      361 ctgtgtgttc attggtcctt cccctgaacg tgcaactacc tgcacgggtc atgtgcttag
      421 tctggtggat atctgccgca tcctaccaga tgcgcgcgga gcttacatca gaccgggagt
      481 gtactcttcg tccgcccgga gtaggtctga agtcccagtt ggggcaagga gcgcccccgg
      541 ccggcccaag cgtctactac ttccggccta agcgcccgtt gcccgaaggt cgtgctaccc
      601 cttacgctat acgctggatg catcgttccc ggtgtctcta tacaatacgc ttgaacatag
      661 caagggcagt caaccgcagc atagcggctt cacgcccttc aaccaggcag tttagccttg
      721 tccgattaaa cacacagtac attaggagga acaaaaagtg aggaaattag tcgtcgtatt
      781 agttgcagcc gcgctcatgc tatccaccgg ggtagccctg gccgagagca cgttcacggt
      841 gccgttcagc cccgtggccg gttccggcgt cgaaggctcg gcggtgttga ccgcggcggg
      901 cgagggcacc gacatctccc tcgacatcac cggcctgacg ccgggcgcac ccgccagcgc
      961 caccctgcac gcgggcacat gcgacgctcc aagcgccagc tttgccgcgc tgcccgacct
     1021 cacagccgat gcgtccggca gggcgacggc cgagggccag gtgcttttca gagggaccga
     1081 aagcgtcgcc ctcgcaagca tagccgatgg ccagcacgtc atgttcatca gccagtccgg
     1141 caaacaggta gcctgcgcgt ggattcccca ggtggagacc gaacccgcct cttccgttgg
     1201 tatgccgagg acgggcggcc tggacctgct gctcgtgctg tccggtctga gtgtggtagg
     1261 tgccatcttc ctggccggtg gattggccct gcgccgccag cactaagtca ccccgaattg
     1321 cgggcttttc ggccatagca acaggggtag tcgaaaccgc aaaggcgcaa agagcgcaaa
     1381 ggacgcggga agataaagta ggtgttctaa gcaaaaggac caggattcgt tattatgtat
     1441 cctggtcctc ttgtgctctc caaacccttg atcttcttag ctgtcctttg cgctctttgc
     1501 gcctttgcgg tttcacctct tcccagattg ctgttgccaa acgctgttgt ctggcataat
     1561 agcggcaggt ccccgattgg cggaggctat ccacgcacgt gaggagacag acatgaccga
     1621 catcccgtgg caagtccgtg gttaccgcga gagcgacatc ccggcgctcg ctgatcttat
     1681 agccgctgcc gaccgggctg acggcgtgca caagctcacc actgaagaag acctgcgcga
     1741 taccttcgag acccccggcc tggaacccgc gcgccaggtc atcgtggccg atggtccgcc
     1801 actaccaggg atccagaagg acgtgttgct gggctttggg agggccttcc ccagcagcga
     1861 caaggccagg aacgagcgga tatacaacgt ggccttgcgc gtgcgtgccg aagtgaggcc
     1921 ctacgcgctc gagcaggaga ttgccaggcg actggtcgaa atcgcccgcc aatccgaggc
     1981 agctctcgag gccccacccg ccgacaaggt gcgcctgagg acctacctct acgacaccca
     2041 cacctccgcc cgccgagcgt gggagcagac cggcctccgg cgggtgcgca ccggctggac
     2101 catgctccgc cccctggacg actcgatacc cggcccccag cccgtcgagg gcgtcacctt
     2161 gcgcacctac cgccgccccg gcgacaaccc cgccgctttg caggccctca atagcgcctt
     2221 ctcggacaat ttcgacttcc aacccctgga tgaggccagg tgggagtacg ccatggcggc
     2281 ctcctacgtg cgccccgacc tctcgtgggt cgcggagccg ctggacgagc cgggtaagct
     2341 ggccggtttc tctatctgct ggataactga tgaggagaac gatcggagcg gccgccggga
     2401 aggctggatc gagggcatcg gggtcgtgcc ggaccggcgc aggcagggca taggccgggc
     2461 gctcctcttg aacagcctcc actctctcag gagcgccggt atggagtcgg ccctcgccga
     2521 cgttgactcc gagagcgagg cacccacccg cctgttccag ttggccgggt tcggcgtccg
     2581 caacagccta tcgcagtacg agtgctacct ggatgaggtg aaagtgtagg ctgcctgtca
     2641 ttctgagcgc agcgaagaat ctaaggaagc tgggcaggga ggctgttgaa catagcatcc
     2701 tatggtgctg acgttcaaca tagaggtaga gggagcatct tagattcttc gctccgctca
     2761 gaatgacaga ggggcgcaca gcccgcccta cccaacatcg agcctacttc tcgccctcga
     2821 tgtagagcat aggcgtgctc atccgcaact ctcctcgctc ggcggagatc cggtttagct
     2881 gcctctccac ctcgccaaac acgctctcct cgtcgccggg gtccaacacg ccgcgccagc
     2941 cgccgaggaa gcctagttga gtgagcgtgt ggttgaacag ggcggtgcca tcggcaaagc
     3001 gcatctcaaa gccgccttcc actaccctcg cgacgcgaaa gcccgcgctc tccaacagcg
     3061 ccgacaccga ctgccgagtg cccctgtgct cctcgttcgc gtccacccta tccaggtagt
     3121 ggggcttgcc cgtgtccgcc agcacggcc
//
LOCUS       DASRWZ010000487         8006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_451167, whole genome shotgun sequence.
ACCESSION   DASRWZ010000487 DASRWZ010000000
VERSION     DASRWZ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451167"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2087
                     /locus_tag="VFH60_07615"
     CDS             <1..2087
                     /locus_tag="VFH60_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313686.1"
                     /translation="DDPRSLQRWPDGLDTNEGSDWAQILKSPGNPSCADPYEQRGGDT
                     TDDVPERAQTHSVGAGNVYLHRLCPTNDTDYISFTASATLTYTVRVTPTAGSQVNAIL
                     RVFDPSGALVREDTDTSTQGASVTFKPAANGIYKAQVANSGGTPGAGPAWLYNINITS
                     TGESGSSASATPTPAGCTDPYEPDDALGAARGIALNSEQVHLLCKPDGSQDTDWVQFP
                     VSSGKVYSFITKNLSGPVDTIISLHNAAGDKLMENDDATPGQGLGSRIDYTFGNTGTY
                     YLRIRDKTGASGPGRQYTVSFESTGQLPPTGTPTASATPNPFSPTPTPGQCYDAFEPD
                     GVMETAKLMYIGSTQRHSICPVGDADWIRFYARPGKVYTIRTLNLGIGVDTYMWVFDG
                     EGKILGYDDDGGGEGVSSRIDFYPLADAFYFVQIKNAGDLGGSEQTYDISLAVAAGVP
                     APPGTATFIVAPVVTVTSGPGEDTTPVAQPTATRPPLPSPTRGVTEPTPASDQPPATL
                     AVPPAQPTQQPTVRVAAPTATTEVVVVPTVEEEPTPGEVIVPGIPATGANASNGSNEP
                     KVIVLPTKVAQKASEIKAPPKQSEPQQLGTDYAPMLFRVFYDRNRNDLFDAGEGIRGL
                     NVYFVGKDARGIALGSLVTEDNGTGRTTLPTSEQRIVIPYLGIDMPLTRFPERELHSI
                     WLSRIALPQRVP"
     gene            2119..3126
                     /locus_tag="VFH60_07620"
     CDS             2119..3126
                     /locus_tag="VFH60_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313687.1"
                     /translation="MSQDDTQHDPHATTTPPDRAAQGDVSTTGHSTYDAQGPAPDAHG
                     DHDDHDEHGDHDGHDAGDTHGCGHGHGPDTSDVPTLVETSWRQLILPALILLLVLILV
                     AGPISGAFSYRPAASPATEQTEPGHEGDGGGTTGSPTTIAQESGATVVVAPSNTTAPS
                     PTKAGATATAPAPTATLSPGDRLSPTQTAVAEAGQNGIVARVPVELNFAGTTFVVNTG
                     DTLLPDWEPSTDPTTATWIKDTVANHVLYVPFSDANLALFTSARTGDLVKLTMNTGQV
                     FEFKVNRAERVANGPPTEEGQFTVTTAMSQDHAGVTLFLIGDPAPDRAVLQAEFTGNI
                     Q"
     gene            3131..3928
                     /locus_tag="VFH60_07625"
     CDS             3131..3928
                     /locus_tag="VFH60_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313688.1"
                     /translation="MASLPPAQQPRFNLEPGQTVIFLGDHTSPDDPGFVRVVREVTAR
                     FYPHLRPNLITAGSPGQTAAALRSQKLMEVLLSSRPDWLAINLGLADVLREPDTRARY
                     AEYVRRKSQAEEGPDATLGPEYRVRPFDLGPESDVGKEPEPRLERLAAFSENLSAAVA
                     SFQGAGIRPILFTTVLLGGDLTSPLNVTLRAYSKAIREIAGERDAPLVEIEKAFREVL
                     DRAANYKQSVTLTDAQGRVNPQGEALIARNFLQTFDLLPGPGFRPKR"
     gene            complement(4340..6763)
                     /locus_tag="VFH60_07630"
     CDS             complement(4340..6763)
                     /locus_tag="VFH60_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6313689.1"
                     /translation="MRDATNSGEAVGDSQQDDTRQSAAPSDNGAASAGEQAAAGPPAP
                     LTTNGPAPSQEGAEAGKRHQRRIVTGSTEPVGTGPLSPPGPSSEPAAPAVLREPGRTN
                     ETDQHYLRREERVKGSHPGDQIIKISRTSGPFRRTRGMLVASLETEAPRTRLGKAFAP
                     VKRLLIGKPLSTEAAIHERLTNVKALAILSSDPLSSVAYATEEILRILIVAGTGALWL
                     AMPVVLAITVLMMIVVASYRQTIAAYPRGGGTYIVAKDNLGRLAGLTAASSILIGYIL
                     TVAVSVAAGVAAIYSLYPELRDQRVPICIAIIVFVTLANLRGVREAGNIFAVPTYLFI
                     IGMFGMIGYGLLRLLFEIGGPMTYTPESTEALPQGTLEGIGLMLLLRAFTQGSAALTG
                     VEAIADGVPAFKPPEAANARKTLISMAVIAITMFVGITFLAVQLKIIPNEHETVVSQL
                     ARSVFGTGGLWYFIQIATALILVLAANTAYADFPRLSYFMARDKFMPHQYSFRGDRLA
                     FSWGIVTLAVFASVLVIVYNGETSSLIPLYAVGVFGAFTMSQSGMVVRWWRMRPPGWK
                     RNFVMNLVGATTTLIVLSVSVFTKLDRGTWIVAVLIPVMIALFLAINRHYARVSLEVA
                     APAEAVSPTTFKHTFIVPVSSLNQVSLAALGYAASLSKNVTAVHIVEGEDPEEAERFR
                     KEWDRLLPDTEVNLVIIESPYRSLIGPLLHYIDARDRQRPDDTITIVLPEVLPSRFWE
                     YLLHNQSALRLKAALLFRSNTVVADMPYILGNQQGTYKRSTIASIPWGAIMALVIVLG
                     AVYMLFFLR"
     gene            complement(6787..7704)
                     /locus_tag="VFH60_07635"
     CDS             complement(6787..7704)
                     /locus_tag="VFH60_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET6313690.1"
                     /translation="MLRQAFLSLSNSQDLQNVALHNGAARKFALRFVAGETLEQAVQA
                     IAALNAKGCKATFDHLGENICNADEARQAAASYIDVLEAIKRYRLDSNVSLKLTQMGM
                     DLDEGLCYENVERICARARELNNFVRIDMESSAYTDRTLDVFRRLWHKGGFRNVGVVL
                     QSYLYRTEGDVREMNKLGARVRLCKGAYNEPSEVAFPRKEDVDINYARLARLLMMEGN
                     YPGIATHDERLIQHLRRYAAKKGVPASKFEFQMLYGVRRSLQLELVKQGYNMRVYVPY
                     GKEWYPYFMRRLAERPANVVFVVGNLLKS"
     gene            complement(7776..>8006)
                     /locus_tag="VFH60_07640"
     CDS             complement(7776..>8006)
                     /locus_tag="VFH60_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313691.1"
                     /translation="GYGGFSAPEGLAPGASTTFEVPIAGFEDANVTDYEGRPDLVSTD
                     QDPPSDATDEQPAAVTPSIIPLATTTRGTTSP"
BASE COUNT         1513 a         2747 c         2401 g         1345 t
ORIGIN      
        1 ccgatgaccc tcggtcattg cagcgctggc ccgatggcct ggacacgaac gagggcagcg
       61 attgggcgca gatcctcaag tcccctggca acccctcctg cgccgacccc tatgagcagc
      121 gcggcggtga tactaccgac gacgtgccgg aacgtgccca gacgcactcg gtgggggcag
      181 gcaacgtcta cctgcaccgc ctctgtccga ccaacgacac cgactatatc agcttcaccg
      241 ctagcgcgac gctgacctac acagtccgtg ttacgcccac ggcaggctcg caggtgaacg
      301 ccatcctgcg ggtgttcgat cccagcgggg cgttggtaag ggaagacacc gacaccagca
      361 cccagggggc ctcggtaacg ttcaagcctg cggccaatgg catatacaaa gcccaggtag
      421 ccaattcggg gggcacccct ggcgcggggc cggcctggct ctacaacatc aatatcactt
      481 ccacggggga gagcgggtct tcagcctccg ccacgcccac gcccgccggg tgcaccgatc
      541 cgtacgagcc tgacgacgcg cttggcgcgg cgcgaggcat cgcgttgaac agcgaacagg
      601 tccacttgct ctgtaagccc gacggcagcc aggataccga ctgggtgcag ttccccgtca
      661 gcagcggcaa ggtctactcc ttcatcacga agaacctttc cgggccggtc gataccatca
      721 tcagtttgca caacgctgcc ggcgacaagt tgatggagaa cgacgacgcc acccccggcc
      781 agggcctcgg ctcgcgtata gactatacgt tcggcaacac cggcacctat tacctgcgca
      841 tccgggacaa gaccggcgca agcggccccg gccggcaata caccgtctcc ttcgaatcca
      901 cgggacagct accgcccacc ggcacgccta ccgcatctgc tacgcccaac ccgttctctc
      961 ccacgcccac ccccggccag tgctacgacg ccttcgagcc ggacggcgtg atggagaccg
     1021 ccaagctgat gtacatcggc agcacgcagc ggcacagcat ctgccccgtg ggcgacgccg
     1081 actggatacg cttctacgct cggcccggca aggtctacac gatacgcacc ctgaacctcg
     1141 gtatcggcgt ggatacctac atgtgggtgt tcgacggtga aggcaaaatc ctcggctacg
     1201 acgacgatgg cggcggcgaa ggcgtctcct cgcgcatcga cttctacccg ttggccgacg
     1261 ccttctactt cgtgcaaatc aagaacgcgg gcgacctcgg cggctccgag cagacttatg
     1321 acatcagcct ggcggtagcg gcaggcgtgc ccgcgccccc cggcaccgcg acgttcatcg
     1381 tggcacctgt ggtcacggtg acctcgggtc ccggtgaaga taccaccccg gtcgcccagc
     1441 cgaccgccac caggccgccc ctgccctcac ctacccgcgg agtgacggag cctaccccgg
     1501 cgtccgacca gcccccggct acccttgccg tgccgcccgc tcagccaacg cagcagccaa
     1561 ccgtgcgtgt cgcagccccg acggccacca ccgaagtcgt ggttgtaccg acggtggagg
     1621 aagagcctac gcctggggaa gtgatcgtgc caggcatacc agccacaggt gccaacgcct
     1681 ctaatgggtc caatgagccg aaggtgatag tgcttccgac caaggttgcg cagaaagcct
     1741 ctgaaatcaa agcgccaccc aagcagagcg agccgcagca gcttggcacg gattatgcgc
     1801 ccatgttgtt ccgggtgttc tacgaccgca atcgcaacga cctgttcgat gcaggcgagg
     1861 gcataagggg cttgaacgtc tatttcgtcg gcaaggacgc ccgtgggata gccctgggca
     1921 gcctcgtcac cgaggacaac ggcaccgggc ggaccacgct acccacaagc gagcagcgca
     1981 ttgtcatccc ctacctgggc atcgacatgc cgcttacccg cttcccggag cgagagttac
     2041 attctatctg gctgtcccgg attgccctgc cgcagcgggt gccctgagca gcaaattcga
     2101 cgcgaggctt gacacgccat gtcacaggac gatactcagc acgaccccca cgccactacc
     2161 accccgcccg accgggcggc gcagggcgac gtgtccacca cgggccactc cacctacgac
     2221 gcccagggtc cggctcccga cgcccacggc gaccatgacg accatgatga acatggcgat
     2281 catgacggcc acgacgcggg cgacacacac ggctgcgggc acgggcacgg ccccgataca
     2341 tccgacgttc cgacgctcgt agaaacaagt tggcggcagc ttatcttgcc tgccctcatc
     2401 ctcttgttgg tgctcatact cgtggccggg cctatctccg gtgccttctc ctacaggccc
     2461 gcagcctccc cggccaccga gcagaccgag ccgggccatg agggtgacgg cggcgggact
     2521 accggctccc ctaccaccat cgcccaggag agcggcgcaa cagtagtagt cgctccaagc
     2581 aacactaccg ccccctcacc caccaaggca ggggcgacgg ccaccgctcc tgcgcccacc
     2641 gccaccctca gcccggggga ccggctcagc cccacccaga cggccgtagc cgaggcgggg
     2701 cagaacggaa tagtcgccag agtgccggtg gagttgaact tcgccggcac aacctttgtg
     2761 gtcaacaccg gcgacaccct cctgcccgac tgggagccgt ccaccgatcc caccactgcc
     2821 acctggataa aggacaccgt agccaaccac gtgctgtacg tgcccttctc cgacgcgaac
     2881 cttgccctct tcacctccgc caggaccggc gacctggtga agctgaccat gaacaccggg
     2941 caggtgtttg agttcaaggt gaaccgcgcc gagcgtgtag ccaatgggcc gcccaccgag
     3001 gaaggccagt tcaccgtcac caccgccatg tcgcaggacc acgcgggcgt caccctcttc
     3061 ctgataggcg accccgcccc cgaccgcgca gtgctccagg ccgagttcac cggcaacatc
     3121 cagtaagcag ttggcgagcc ttcccccggc ccaacagccc cgcttcaacc tggagccggg
     3181 ccagaccgtc atcttcctgg gcgaccacac ctcccccgac gaccccggct tcgtccgcgt
     3241 ggtgcgcgag gtaaccgccc gcttctaccc acacctgcgc cccaacctca tcaccgccgg
     3301 ttcgccaggc cagacggccg cagccctgcg ctctcaaaag ctgatggagg tgctgctctc
     3361 atcgaggccc gattggctgg cgataaacct ggggctggcc gacgtgctgc gcgagccgga
     3421 caccagggcc aggtacgccg aatacgtcag gcgaaagtcg caggccgaag aggggccgga
     3481 cgccacgctg gggccggagt accgggtgcg gcccttcgac ctgggtccgg agtccgacgt
     3541 gggtaaggag ccggaaccac gattggagcg gctggcggca ttcagcgaga acctgtccgc
     3601 cgctgtagcc agtttccagg gcgcgggcat acgccccata ctcttcacca ccgtgctgct
     3661 gggcggcgac ctcacaagcc ccctcaacgt caccctgcgc gcctatagca aggccatccg
     3721 cgagatagcg ggggagcggg acgcgccgct ggtggaaata gagaaggcgt ttcgcgaggt
     3781 gctggacagg gcggccaatt acaagcagag cgtgacgctg accgacgccc agggccgcgt
     3841 gaacccacag ggcgaagccc tcatcgcccg caacttcctg caaaccttcg acctcctccc
     3901 cggacccggc ttcaggccca agcggtaacc tcacaccgtg cagacaatcg cgcaccgcct
     3961 ccccctgtgg ctaaagccac gggctgggaa gtagcggagt ctcctacgga gactctctac
     4021 atgaggatac ggactatgta cgccctggag cagacgagcg aacaaaggtg acccctgtca
     4081 ttctgagcgt agcgaagaat ctaagctgct ggcatagcct ctacattgaa cgtcagcacc
     4141 ttagtacgtt acgttcaaca gcctccctgt gccaacttcg ttagattctt cgctgcgctc
     4201 agaatgacag gggccgcttt ggtcccgcaa acgctgcact ttgcagctac tccccagtct
     4261 ccgaaggagg cttcgctact tcccagcccg tggcttcagc cacagggcga acgcggcaga
     4321 gcgactcggc atgaattgtc taccgcagga agaacagcat gtacacagcc cccagcacga
     4381 taaccagggc cattatcgcg ccccacggga tagatgcgat ggtcgagcgc ttgtacgtcc
     4441 cctgctggtt gcccaggatg taaggcatgt cggccaccac cgtgttggag cggaagagca
     4501 gggccgcctt cagcctcagc gcgctctggt tgtggagcag gtactcccag aagcgcgacg
     4561 gcagcacctc cggcagcaca atcgtgatcg tgtcgtccgg cctctggcgg tcccgcgcgt
     4621 cgatgtagtg caacagtggg cctatcagcg agcggtaggg cgactcgatg atcaccaggt
     4681 tgacctcggt gtccggcagc agcctgtccc actccttgcg gaaacgctcc gcctcctccg
     4741 ggtcctcgcc ctccacaatg tgcaccgccg tcacgttctt gctcagcgaa gcggcgtagc
     4801 ccagcgcggc cagcgatacc tggttgaggc tcgacaccgg cacgatgaag gtgtgcttga
     4861 acgtcgttgg ggacactgct tcagcggggg ccgccacttc cagggagacg cgggcgtagt
     4921 gccggttgat cgccaggaac agcgctatca tgaccggtat caacaccgcc actatccacg
     4981 tgccgcggtc gagcttcgtg aacaccgaca cgctgagcac gatcagggtg gtggtcgcgc
     5041 ctacaaggtt catcacgaag ttgcgcttcc accccggcgg ccgcatgcgc caccagcgga
     5101 ccaccatgcc gctctgtgac atcgtgaacg ccccgaacac ccccaccgcg tacaagggga
     5161 tcagcgaaga ggtctcaccg ttgtacacga ttacgagcac ggacgcgaag acggccagcg
     5221 tcactatgcc ccaggagaag gccagccggt cgccccggaa cgagtactgg tgcggcatga
     5281 acttgtcgcg ggccatgaag taagacaggc gcgggaaatc ggcgtaggct gtgttggccg
     5341 ccagcaccag gatgagcgcg gtggctatct gtatgaagta ccacaggccc ccggtgccaa
     5401 acacgctcct cgctagctgc gatactaccg tctcgtgctc gttcgggatg attttcagtt
     5461 gcaccgccag gaacgtgatg ccgacgaaca ttgtaatagc tattaccgcc atagagatga
     5521 gagtcttgcg ggcgttggcg gcctcgggtg gcttgaacgc gggcacgccg tcagctatag
     5581 cttccacacc cgtcagcgcc gccgaaccct gggtgaacgc cctcaggagg agcatcaacc
     5641 ctataccctc caacgtgcct tgcggcaaag cttcggtcga ttcgggcgta tacgtcatgg
     5701 ggccgcctat ctcgaataag agccggagca agccatagcc tatcatgccg aacatgccaa
     5761 ttatgaacag gtacgtcggc acggcgaaga tattgcccgc ctcgcgcacg ccgcgcaggt
     5821 tagccagggt gacaaaaact atgatggcga tacaaatggg cacgcgctgg tcccgcagct
     5881 cgggataaag cgagtagatt gccgccacgc ccgccgccac gctcaccgcc acggtgagga
     5941 tgtagccgat gaggatcgag gacgccgccg taagcccggc cagcctgccc aggttgtcct
     6001 tggcgacgat ataggtgccg ccgcccctgg ggtacgcggc aatcgtctgc cggtaggagg
     6061 ccaccacgat catcatgagc accgtgatgg cgagcactac cggcatggcg agccacagcg
     6121 ccccggtccc cgccacgatg aggatgcgca ggatttcctc ggtcgcgtag gccaccgagg
     6181 aaagcgggtc ggacgagagg atggccagcg ccttgacgtt ggtcaggcgc tcgtgtatgg
     6241 ccgcttcggt gctcaggggc ttgcctatca gcagccgctt gaccggggcg aaggctttac
     6301 ccaggcgcgt gcgcggggcc tccgtctcca gcgaagccac cagcatgccc ctggtgcgtc
     6361 tgaacgggcc gctggtgcgg cttatcttga ttatctggtc cccagggtgg ctccccttga
     6421 cgcgctcttc ccgccgtaga tagtgctggt cggtctcgtt ggtacggccc ggctccctga
     6481 ggacggccgg cgcggccggc tcggacgagg gcccgggcgg gctaagtggc cctgtaccca
     6541 ctggctcggt ggacccggtg actatgcgcc tctggtgccg cttgcccgcc tcagcccctt
     6601 cttgagaagg cgcgggtccg ttcgtggtaa gcggtgcggg cgggccggca gcggcttgct
     6661 cgccagcgga ggccgcgccg ttatccgacg gggccgcgct ctgccttgta tcgtcctgtt
     6721 gtgagtcgcc cacggcctcg cctgagttgg tcgcgtcgcg catatcgaac taagcccccc
     6781 ggctctctag ctcttgagga gattacccac tacgaatacc acgttggcgg gccgttccgc
     6841 cagcctgcgc atgaagtagg ggtaccactc tttaccgtag ggtacgtaga ccctcatatt
     6901 gtagccctgt tttaccagct ctaattgcag gcttcggcgc accccgtaga gcatctgaaa
     6961 ctcaaacttc gacgccggga cgcccttctt ggccgcgtat cgcctgagat gctgtatcag
     7021 ccgctcgtcg tgcgtggcga tccctggata gttgccctcc atcatgagca ggcgggccag
     7081 gcgtgcatag tttatgtcaa catcttcctt gcgggggaaa gctacctcgg aaggctcgtt
     7141 gtacgccccc ttgcagagcc gcacccgcgc ccccagcttg ttcatctcgc gcacgtcgcc
     7201 ctcggtgcgg tataggtacg actgcaacac cacacccacg ttgcggaagc cccctttgtg
     7261 ccagagcctg cggaacacgt cgagggtgcg gtcggtgtag gccgagcttt ccatgtcaat
     7321 ccgcacgaag ttattcaatt cccgtgcccg cgcgcaaatc cgttccacgt tctcgtaaca
     7381 aagcccctcg tcaaggtcca tccccatctg cgtgagcttc agcgacacgt tggagtcgag
     7441 cctgtaccgc ttgatggcct caaggacgtc tatatagctt gccgctgcct ggcgggcctc
     7501 gtcggcgttg cagatgttct cgcccaggtg gtcgaacgta gccttgcaac ccttagcgtt
     7561 cagggcggca atcgcctgca ccgcctgctc caatgtctcg cccgccacga agcgcagtgc
     7621 gaacttgcgg gccgcgccgt tgtgcagggc cacgttctgc aagtcctggc tattcgagag
     7681 cgataagaag gcctgtcgta gcactgtcct ctcctttgaa gaaagcatta aagcaaaatt
     7741 ggcccggccc atagcaggcc gggccaatca caaatctacg ggctggtggt gcctcgcgtg
     7801 gtggtggcaa gcggaataat cgacggtgtg accgccgcgg gctgctcgtc cgtcgcgtcc
     7861 gacggcgggt cctggtcggt ggacaccagg tcggggcggc cctcatagtc ggtgacgttg
     7921 gcgtcttcga agcccgcgat gggcacctca aacgtggtcg atgcccccgg cgcaaggccc
     7981 tccggcgcgc taaacccacc gtagcc
//
LOCUS       DASRWZ010000488         5246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1273788, whole genome shotgun sequence.
ACCESSION   DASRWZ010000488 DASRWZ010000000
VERSION     DASRWZ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273788"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..1017
                     /gene="pth"
                     /locus_tag="VFH60_07645"
                     /pseudo
     CDS             430..1017
                     /gene="pth"
                     /locus_tag="VFH60_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512912.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            1283..5089
                     /gene="mfd"
                     /locus_tag="VFH60_07650"
     CDS             1283..5089
                     /gene="mfd"
                     /locus_tag="VFH60_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET6313692.1"
                     /translation="MSLSGLLPLLYDQRGYGAVARAVAAGENAWAEAPMDAARPYVLA
                     ALHTDVTAPASRPMIVVAPRSERARQLYEGLLAYSPPGTPILFFPAPDLLPYERIAPD
                     PTITGERLRVIAMLHQPELRGQATRADGQGSGSIAAALQELVQNPATGSQPHAPIIVT
                     SVFALMTPTLAPDDLRHAIRVIRRGERVVMSDLLSHLVGLGYEPAALVEEPGQFGRRG
                     GIVDLFPPTSNLPIRVEFFGDEVESIRQFNPLTQRSEGQANAIVITPSCELPLWKHER
                     AYESLRDIDTANLREEVLEEWRDQLEKTGRGECFEGQELFAPFYTQPLASLADYLAAL
                     RSTGDGNGKPPLLVLDDPELIRLEASEIERQASELYRGFIDNGELPPGLQQPYLTWEE
                     VAAHGRGLPTLSIGGGAEGAIEAPPFSGTRQYTGSIPNVITDLKEYIANRSRIVIVSQ
                     QSSRLRELLEDEGIYPTLRKGRRPDDLHAVPGNRNIVGVEPVALWQGPGGLNEALLAP
                     PSPGTVNLLQGSLAAGWQLMQGTHTDTLLLTDMELFGRSQTGVRRATGQARRSTSDAA
                     AILREKMLLELKPSDYVVHVEHGIARFGGLVHMTAGGADREYMLLLYAEGDRLYVPAD
                     QTDRVAPYIGAGPPPALHRLGTAEWSRTKRKVREAAELLARDLLELYANREAAGGFQY
                     SPDTTWQYELEESFPFVETPDQLRSIAEVKTDMEEPRPMDRLICGDVGYGKTEVALRA
                     AFKAVMDGRQVAVLVPTTVLAQQHLNTFTERLAAFPVTVSMISRFRSRQEQTRIVKDL
                     AEGKIDIIIGTHMLLSKSIQFKNLGMVVVDEEQRFGVRHKERLKQLRAEVDVLTLSAT
                     PIPRTLYMAISGVRDLSLIETPPEARLPIKTYVTAFRPQLVREVILRELERGGQVFFV
                     HNRVETIDKLAYELEALVPEARFIVGHGQMHEDMLEKVMQKFVAREADVLICTTIIES
                     GLDIPNANTLIVDHADKLGLAQLYQLRGRVGRGANRAYAYFLYHAGRKVTVTARERLS
                     TIEQATELGSGFRIALRDLEIRGAGNLLGPEQSGHVAAVGLDLYTRLLASAVERVRSE
                     RKRAQRTGNTDDQTAVATQPNPKSEIQNPKSEEPPAVSIDLPITAFLPEDYVPDAAVR
                     LRLYQRMAASMTPSQVRDFGRELQDRFGTLPEPASNLLEVVRLKGLAITAGVESIRAL
                     AEEYLIVVPEDMPIPEHIRMRLQRKHKDLLKTSPHQVRISRARAGEKWQDVLTEVLEE
                     MGEAQG"
BASE COUNT         1061 a         1719 c         1638 g          828 t
ORIGIN      
        1 gaggccggta ttttcaacga tactgcccaa tttgtgcttt atcttgtcga ttgctacgag
       61 caaggtgacg tcgcaacttt gcgctcagca ttcgacacga ttgagaggtt tattgcatag
      121 ggaacagaag atacgcggga aatagcaata gttggcatac tggaaaccct ccagaactat
      181 gcatctcacc gcccctacgg agaaggggct ttcgttgagt tcctgggtcc caagtcattg
      241 gctgcatgga atcaactggt ctatctatgg gaggggaaga gcagtctggc tgatgtaatt
      301 agagcagaat acaggagaga tgcttaaaat cgtcgcattc ttgcagcgaa acaatcttca
      361 agatgtgttc ggcaggaata aaaacctcag attgaatccg catggctgcc aagtttataa
      421 tggagggcta tgcagcaaaa cgtctcacaa gaaaacctcc ccctcttgat cgtcggcctg
      481 ggcaaccctg gctctaagta cgccgctacc cgccacaacg tcggcttcat gacggtggac
      541 gagcttgcgc ggcggcacgg cctgcgcttc tccacaaagc aagcagacgc cgagatcgcg
      601 cggggcgagc tagagggcac ccgcgtcata atcgccaagc cgcagacctt catgaacaac
      661 agcgggcggg cggtgggcgc gctggtacgc ttctacaagc tgccttccag ccgcgtggtg
      721 atcatctacg acgagctaga cctgccggtg ggcaccatac gcatgcgcgg caaggggtcg
      781 gccaacgggc ataacggcct caagagcgtg atacagcacc tcggcacgca ggagttcccc
      841 cgcatccgca ttggcatcag caggcccgcc atcaccgacc acaagcagat agactgggtg
      901 ctgggccgct tcacgaagga cgagcaggag gtgatggacg acactatccc ccgcgccgcc
      961 gaggccgtgg aagccatcat cacgctgggg atggagcggg cgatgaacag gtacaacacg
     1021 agggaagagc agggcaaacc cgcctccaag cccgcacctg agccaactcc gccagacctc
     1081 gcacagcgta taaaagaggc agagtccccg gcccctccca ggaaggaaag ctgggtagac
     1141 aggatacgcc acatcatccg ggaggaagcg aagtgacatt tgggaatcca gattttagat
     1201 tttgggtttt ggattttgga ttgcagaaag accagtcgaa aatccaaaat ccaaaatcta
     1261 aaatccaaaa tcggaggtcc cgatgagcct ttcgggactg ctgccgctac tgtacgacca
     1321 gcgcgggtac ggggccgtgg cgcgggcggt ggcggcgggc gagaatgcct gggcggaagc
     1381 cccgatggac gcggctcggc cttacgtgct ggcagccctc cacaccgacg taacggcccc
     1441 ggcgagccgc cccatgatag tggtcgcgcc ccggtcggag cgcgcgcggc agctatacga
     1501 gggtctgctt gcctactcgc cccccggcac gcccatcctc ttcttccccg cccccgacct
     1561 gctgccctac gagcgcatcg ctcccgaccc cactatcacg ggcgagcggc tgcgcgtgat
     1621 cgccatgctg caccaaccgg agttgcgcgg acaagccacg agggctgacg ggcagggttc
     1681 aggctcgatt gccgctgccc tgcaagagct tgtgcagaac ccggcgaccg gctcccagcc
     1741 ccacgcccct attatcgtca cctccgtctt cgcgctgatg acccccacgc tcgcacccga
     1801 cgatttgcgg cacgcgatca gggtaatcag gcgcggcgag cgggtggtga tgagcgacct
     1861 gttgagccac cttgttggcc tggggtacga acccgccgcc ctggtggagg agccggggca
     1921 gttcggcagg cgcggcggca tcgtggacct gtttccgccc acctccaacc tgcccatccg
     1981 cgtggaattc ttcggggacg aggtagagtc gatacgccag ttcaacccgc tcacccaacg
     2041 gtcggagggg caggccaacg cgattgtgat cacgccttcc tgcgagttgc ccctctggaa
     2101 gcacgagcgg gcatacgaga gccttcgcga catcgatacg gccaacctgc gcgaggaagt
     2161 gctcgaggag tggcgcgacc agctagaaaa gaccgggcgg ggcgagtgct tcgaggggca
     2221 ggaactgttc gctcccttct acacacagcc ccttgcaagc ctggccgact acctggccgc
     2281 gctccggagc acgggtgacg gcaacggcaa accgcccctg ctcgtgctgg acgatccgga
     2341 gctgatacgc ctggaagcct cggagataga gcggcaggcc tcggagcttt accggggctt
     2401 catcgacaac ggcgagctgc cccccggact tcagcagccc tacctcacct gggaagaggt
     2461 agcggcgcac gggcgggggc tacccaccct ctccatcggt ggcggcgcgg agggggcaat
     2521 cgaagccccg cccttcagcg gcacccggca gtacaccggc agcatcccga acgtaatcac
     2581 cgacctcaag gagtacatag ccaacaggtc gcgcatcgtg atagtgtcgc agcagtcgag
     2641 ccgcctgcgc gagctgctgg aggacgaggg tatctacccc acgttgcgca agggcaggcg
     2701 gcccgacgac cttcatgccg tgccgggcaa ccgcaacatc gtgggcgtgg agccggtggc
     2761 gctgtggcag gggccgggcg ggctgaacga ggcgctgctg gccccgcctt ctcccggcac
     2821 ggtcaacctg ctgcaaggct cgctcgccgc cgggtggcag ctgatgcagg gcacccatac
     2881 cgacaccctg ctgctcaccg acatggagct attcgggcgc tcgcagacgg gggtgcgccg
     2941 cgccaccggc caggcgcgcc gctcgacctc cgacgccgcc gctatcctgc gcgagaagat
     3001 gctgctggag ctgaagccga gcgactacgt ggtgcacgtg gagcacggca tcgccaggtt
     3061 cggcggcctg gtgcacatga ccgccggggg ggccgaccgc gagtacatgc tcctcctcta
     3121 cgccgagggc gaccgcctgt acgtgcccgc cgaccagacg gaccgcgtgg ccccctatat
     3181 cggcgcgggg ccgccccccg ccctccaccg cctgggcacc gccgagtggt cgcgcaccaa
     3241 gcgcaaggtg agggaggccg ccgagctatt ggcccgcgac ctgctggagc tatacgccaa
     3301 ccgcgaagcc gccggagggt tccagtattc gcccgacacc acctggcagt acgagcttga
     3361 ggagagcttc cccttcgtgg agacgcccga ccagttgcgc tccatagccg aggtcaagac
     3421 cgacatggag gagccgcgcc cgatggaccg cctcatctgc ggggacgtgg gctacggcaa
     3481 gaccgaggtg gcgttgcgcg cggccttcaa ggcggtcatg gacgggcggc aggtggccgt
     3541 gctggtgccc accaccgtgc tcgcccagca acacctcaac actttcaccg agcgcctggc
     3601 tgccttcccc gtcaccgtgt ccatgatttc gcgcttccgc tcccggcagg agcagaccag
     3661 gatagtgaag gacctggccg aaggcaaaat agacatcatc atcggcacgc acatgctgct
     3721 gtcgaagtcg atacagttca agaacctcgg catggtggtg gtggacgagg agcagcgctt
     3781 cggggtgcgg cacaaggagc ggctgaagca gctacgcgcc gaggtggacg tgctcaccct
     3841 ctccgccacg cccatcccgc gcaccctcta catggctatt tcgggcgtgc gcgacctctc
     3901 gctgatagag accccgcccg aagctcgcct gcccatcaag acctacgtga ccgccttccg
     3961 cccccagctc gtgcgcgaag tgatactgcg ggagctagag cgcggcgggc aggtcttctt
     4021 cgtgcacaac cgggtggaga cgatagacaa gctggcctac gagcttgagg cgctggtgcc
     4081 cgaagcccgc ttcatcgtgg gtcacggcca gatgcacgag gacatgctcg aaaaggtgat
     4141 gcagaagttc gtggcccgcg aggccgacgt gctcatctgc accaccatca tcgagtcggg
     4201 cctcgacata cccaacgcca acaccctcat tgtggaccac gccgacaagc tggggctggc
     4261 gcagctatac cagcttaggg gccgggtggg acgtggggcc aaccgcgcct acgcctactt
     4321 tctgtaccac gcgggccgca aggtgacggt gaccgcccgc gagcgcctgt ccacaatcga
     4381 gcaggccacc gagctggggt ccggcttccg catcgccctg cgcgacctcg aaatccgggg
     4441 cgcgggcaac ctgctcggcc ccgaacagag cggccacgta gccgccgtgg gcctagacct
     4501 ctacacccgc ctactcgcct ccgccgtgga gcgcgtccgc tccgaacgca aacgcgccca
     4561 acgcacaggc aacactgacg accaaactgc cgtagccacc caacccaatc caaaatccga
     4621 aatccaaaat ccgaaatccg aagagcctcc cgccgtatcc atcgacctgc ccataaccgc
     4681 gttcctgccg gaggactacg tgcccgacgc cgccgtgcgc ctgcgtctct accagcgcat
     4741 ggccgccagc atgacccctt cccaggtgcg cgatttcgga cgagagttgc aggaccgctt
     4801 cggcacgctg cccgaacctg cctccaacct gctcgaagtg gtgcgcctca agggcctggc
     4861 aatcaccgcc ggggtggagt cgattcgcgc ccttgcggaa gagtacctca tcgtggtgcc
     4921 ggaggacatg ccgattccgg agcacatccg catgcgcttg caacgcaagc acaaagacct
     4981 cctcaagacc agcccccacc aggtgcgcat cagccgcgcc cgcgcaggcg agaagtggca
     5041 ggacgtgctg acagaggtgc tggaggagat gggggaggcg caaggctgaa acgcgacacg
     5101 tgaggcgggc tgccaaggat gaaggcggta tgttccggtt gaattgcagg accaagagtg
     5161 gccttgtcat tctgagcgta gcgaagaatc taaagaagca gctaaaggga ggctgttgaa
     5221 ccataagtac catgatcctg acgttc
//
LOCUS       DASRWZ010000489         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_862898, whole genome shotgun sequence.
ACCESSION   DASRWZ010000489 DASRWZ010000000
VERSION     DASRWZ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862898"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..2280)
                     /locus_tag="VFH60_07655"
     CDS             complement(88..2280)
                     /locus_tag="VFH60_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET6313693.1"
                     /translation="MPSSEAKGPKSVAVLGAGPAGLYFALLAKKADPSCDITVIERNP
                     ADVTYGWGVVFSDRTLASFQRADFKTYERITENFVIWDAIDVRYRGETIRCGGHVIAS
                     ISRKLLLNILQERCAELGVKMVFGREINDLGELGGDYDLLVAADGINSLARRQYEGEF
                     KPRMEMGRARYIWLGADKVLDAFTFIFHETEHGLFQVHAYPYSGTSSTFIVECAEDTW
                     QRAGLDRATEEESLAFCQRLLNDDLHGAPLLSNNSKWISFPTLKTRNWHTEIQSLPSS
                     EAKGPKSKIQNLVLLGDAAHTAHFSIGSGTKLAMEDAIALANALEQHPDVGKALDEYE
                     LERKPVVETFQRAAHESQTYFETLKRYLVLDPVPFAFQLLTRSGRITYDDLHFRDARF
                     GDMVDRWMHGRGTARVAPPPMFAPLELGSTCLPNRVVMSPGPEGDGGGAPADGYIARL
                     AECAGGGAGLVLAGTCAVSPEGRVTPADLGLYDEAHGGAWAAAVAKVHAIQGARIGVR
                     LGHAGRRGSTRPRSEGLDRPLKEGNWLLISASAIPYTPAGQVPKEMDRADMDRVRDDF
                     ERATRMAYAAGFDLLQLDFAHGYLLAGFLSPLTNRREDEYGGSLENRARYPLEVLAAV
                     RAVWVDRPLSVVITATDYVKGGLEVEDAIEVARMLEASGCDLAQVHAGQSTPEAEPPY
                     ARGFLTPLADRIRNEAGIPTLVGGYLTTSNEANTVLAAGKADLCLMEV"
BASE COUNT          393 a          797 c          769 g          469 t
ORIGIN      
        1 gcatcttaga ttcttcgcta cgctcagaat gacagagggg gcttacatcc cgacgttcgg
       61 caatcttgtt cgtgaggtga cttcccctca tacctccatc aaacatagat ctgccttgcc
      121 cgccgccagg actgtgttcg cctcgttgct ggtggtgaga tagccgccta ccagcgtggg
      181 gatgcctgcc tcgttgcgga tgcggtccgc taggggggtg aggaagcccc tggcgtatgg
      241 cggctcggcc tcgggcgtgc tctgccccgc gtggacctgc gccaggtcgc agccgctcgc
      301 ctcgagcatg cgcgccacct caatcgcgtc ctccacttcc aacccgccct tcacgtaatc
      361 ggtggccgtg atgactaccg acagcggcct atcgacccac actgcccgca ccgccgcgag
      421 cacctcaagc gggtagcggg cgcgattttc caggctgccg ccgtactcgt cctcgcgccg
      481 gttggtgagg ggcgacagga agccggcgag caggtagccg tgcgcgaagt cgagttgcag
      541 caggtcgaag cccgccgcgt acgccattcg ggtcgcccgc tcaaaatcgt cgcgcacgcg
      601 gtccatatcc gcccggtcca tctctttggg cacctggccg gcgggcgtgt aagggatagc
      661 ggaggcggat atgaggagcc aattaccttc cttgagcggc ctgtccaggc cctccgacct
      721 ggggcgggtg gagcctcgcc gccctgcatg ccccagcctc acgccaatgc gggcaccctg
      781 gatggcgtgc accttcgcga ccgccgcagc ccacgcccct ccgtgcgcct cgtcgtacag
      841 tcccaggtcg gcgggcgtca cgcgcccctc cggagatacc gcgcacgtac ccgccagcac
      901 caaccccgcc ccgcccccgg cgcactccgc caaccgcgct atatagccgt cagcgggcgc
      961 gccaccccca tcgccttcgg gcccgggcga catcacaacc ctgttcggca ggcaggttga
     1021 ccccagctca agcggtgcga acatgggcgg gggcgcgacc ctggccgtcc cacgtccgtg
     1081 catccacctg tccaccatgt cgccaaagcg cgcgtcgcgg aagtgcaggt cgtcgtaggt
     1141 gatgcggccg ctacgcgtga ggagctggaa ggcaaagggc accgggtcga gcacgaggta
     1201 ccgcttcaac gtctcaaagt aggtctggct ttcgtgcgcc gcccgctgga acgtctccac
     1261 caccggctta cgctccagct cgtactcgtc gagcgccttc ccaacgtcgg ggtgctgctc
     1321 gagcgcgttc gccagggcaa tcgcgtcctc catagccagc ttcgtgcctg acccgatgga
     1381 gaagtgggcc gtgtgcgccg cgtcacccag gaggactaga ttttggattt tggattttgg
     1441 acccttcgct tcgctcgagg gcaggctttg gatttcggtg tgccaattcc tggtttttag
     1501 ggttgggaag ctgatccact tggagttgtt ggataggagt ggtgcaccgt gcaggtcgtc
     1561 gtttagcagg cgctgacaga aggcgaggct ttcttcctcg gtggcccggt cgaggcccgc
     1621 gcgttgccag gtgtcttcgg cgcactccac gatgaaggtg ctggacgtgc cgctgtaggg
     1681 gtaagcgtgc acctggaaca ggccgtgctc ggtctcatgg aagatgaagg tgaaggcatc
     1741 cagcaccttg tcggccccca gccagatgta ccgcgcccgc cccatctcca tgcgtggctt
     1801 gaactcgcct tcgtattgcc tgcgggccag gctgttgatg ccgtccgccg ctaccagcag
     1861 gtcgtagtca ccgcccaact cccccaggtc gttaatctct cgcccaaaga ccatcttcac
     1921 gcccaactcg gcgcaccgtt cctgcaatat gttgagcagc agcttgcgcg agatgctggc
     1981 gattacgtgc ccgccgcaac ggattgtctc gccccggtag cgcacgtcta tagcgtccca
     2041 gatgacgaag ttctcggtaa tgcgctcgta ggtcttgaaa tcggcgcgct ggaacgaggc
     2101 gagcgtgcgg tcggagaaga ccacccccca gccgtaggtc acgtccgccg ggttgcgctc
     2161 gatgaccgtg atgtcacatg acgggtcggc tttcttagcg agcagggcaa agtacagccc
     2221 ggcggggccg gcgccgagga cggcaaccga ttttggaccc ttcgcttcgc tcgagggcaa
     2281 gctttggatt ttggattttg gattttgggt cacggggacc tctaagggtg gttcaggcgg
     2341 gtactcgtag gctggtgagt acagcagcta attgctctag ctgggctatg tcggggatag
     2401 cggggaagag caccatctcg tcgcagcc
//
LOCUS       DASRWZ010000490         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_588636, whole genome shotgun sequence.
ACCESSION   DASRWZ010000490 DASRWZ010000000
VERSION     DASRWZ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588636"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1228)
                     /gene="hemW"
                     /locus_tag="VFH60_07660"
     CDS             complement(11..1228)
                     /gene="hemW"
                     /locus_tag="VFH60_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET6313694.1"
                     /translation="MLIANHKPKTSAPTAPYDWRDEPVGLYLHIPFCESKCIYCDFNS
                     YAGMEHKFEPFVRALVRDIERGASWDLPGAPNCEGAEVTTIFFGGGTPSVLTPGQIGR
                     ILAAARQRYSIVPGAEVTMEANPGTISLKKFEGYLGAGVNRLSMGVQVLDDLMLKKLG
                     RIHTAEGALESYRLARQAGFENINLDFIYGLPGQDLAHLQSVVERLLEVDPLPDHLSC
                     YSLIVEENTPLFTGVERGLISVPGQDDVADMYEHICRRLPGAGYEQYEISNWSMGKPC
                     AHNLVYWHDGRYLAFGPGASGYWGDARYTTLLSPGEYIKGVVQGESVIAERTEVSRQD
                     EMSEVMMLGLRLNEGVSLGYFEQRFGVALHEVYGRVVDEFVRIGLLQEVAGWVRLTER
                     GRLLGNEVFARFI"
     gene            1558..2562
                     /locus_tag="VFH60_07665"
     CDS             1558..2562
                     /locus_tag="VFH60_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET6313695.1"
                     /translation="MTTYTAPKSNLTLKQILQPVAADIAGIDDILRSISTLDNPLLND
                     MVEAIIAAGGKRLRPAILIMSAHFNDFQLERLLPMSAAIELLHTATLIHDDTIDEALV
                     RRGLPTLNSLIDSGTTVLVGDYIFAKSAVLSTMGGKPRATRIFADTLVTICEGEIEQK
                     FSTHHVSRSVEQYYKRIYSKTAVLFAAAGELGAVLSDAPEDQVQSLRHYGKQVGMAFQ
                     IVDDILDLRATTQQLGKPTGGDLRQGTVTLPTMYYLETADDEGREVITRVIENDERTD
                     GLVERAIRLINESGAVERAHAEAVNFVEEAKAALTRLPDIPIKQSLIDLADFVVRRDF
                     "
     gene            2734..>3433
                     /locus_tag="VFH60_07670"
     CDS             2734..>3433
                     /locus_tag="VFH60_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6313696.1"
                     /translation="MDGVLKGLRPYSPDDLPQLRRLLTETQAWPPVATPADEDILTRW
                     KRRGVIPQNDISVLPGPRGELVAFSQGFRLAHGVSRFSFEIGVRPSHRQQGIGGALYR
                     LVEARARDMGVANMTSPVFVTSGVARPECSGFLERRGFRVESSYWQMRVDDLVRQAPA
                     RWPEGIECRPFGDPHRDAPRWAQLIRDTFNEHATAEGILQQLSEEGVLRHGYFFAVDR
                     ATGLEVGTTRSRIDY"
BASE COUNT          679 a         1159 c          955 g          640 t
ORIGIN      
        1 cacgcgcctg ctagataaac ctggcgaata cctcgttgcc cagcagccta ccccgctccg
       61 tcagccgcac ccacccggcg acctcttgaa gcagacctat cctgacaaac tcgtccacga
      121 cccgcccgta gacctcgtgg agcgcaacgc caaaccgctg ctcgaagtag cccaggctca
      181 caccctcgtt caagcgcagg ccgagcatca tcacctccga catctcgtcc tgccgcgaca
      241 cttcggtgcg ctccgcaatc acgctctccc cctgcacgac ccccttgatg tattcgccgg
      301 ggctgagcaa ggtcgtatag cgcgcgtcgc cccagtagcc gctcgcgccg gggccgaacg
      361 ccaggtagcg cccgtcgtgc cagtagacca ggttgtgagc gcacggcttg cccatgctcc
      421 agttggagat ctcgtactgc tcgtaccccg cccccggaag cctccggcag atgtgctcgt
      481 acatgtcagc cacgtcgtcc tgccccggca ccgagatcag cccgcgctcc acccctgtga
      541 agaggggcgt gttctcctcc acgatcagcg agtaacatga caggtggtcc ggcagcggat
      601 cgacctccag tagccgctcg accaccgact gtagatgcgc caggtcctgg cccggtaggc
      661 cgtagatgaa atcgaggttg atgttctcga accccgcttg ccgggcgagc ctgtacgact
      721 ccagcgcgcc ttcagcggtg tgtatgcggc ccagcttctt cagcatgagg tcgtcaagca
      781 cctgcactcc catgctcagc ctgttcacgc ccgcccccag gtagccctca aacttcttca
      841 ggctgatcgt gccggggttg gcctccatag tcacctcggc ccccggcacg atgctgtacc
      901 tctgcctggc ggcggcaagg atgcggccaa tctgccccgg tgtgagcacg ctgggagtgc
      961 ccccgccaaa gaagatagtg gtgacctccg ccccctcgca gttgggagcc cccggcaggt
     1021 cccagcttgc cccccgctct atatcccgta ccagggcgcg cacgaacggc tcgaacttgt
     1081 gctccatccc cgcgtacgag ttgaagtcgc agtagatgca cttcgattcg caaaacggta
     1141 tgtgcaggta cagcccgacc ggctcgtccc gccaatcata gggtgcggtg ggcgctgaag
     1201 tctttggttt gtggtttgca attagcatag cttgacttgt ggtgctggcg cagtctattt
     1261 gctagaagct atctcgtaaa ggaccagtgt gaaacattag cagcaaagcc ccttctatgt
     1321 catttcgaac ccttcggggg cgctcagggt gggccttacg cgttgaagtt caactatcat
     1381 accttgacgt gactgctatc gccttcttaa cccgaatccc tcaattatac gggctacgaa
     1441 gtcgaacaag caaagcggcg aaaaagattg tgataatctt gacaatcttt ctgcggcttg
     1501 atatactcat agtgagcgtt caggccgacc agcaatgcca cagagagcgt gcaatctatg
     1561 acaacctaca ccgcccccaa atctaacctg actctaaaac agattctgca gcctgtggcc
     1621 gcggatattg ccggcataga cgatattctc cggtctatca gcacgctcga caatcccctg
     1681 ctgaacgaca tggtcgaggc cattatcgcg gcggggggca agcggctcag gcccgccatc
     1741 ctgatcatgt cggcccactt caacgacttt cagttggagc ggctgctgcc gatgtcggcg
     1801 gctatcgagc ttctgcacac cgccaccctg atccacgatg acacgattga cgaggcgttg
     1861 gtacgacgcg gcctacccac gctcaattct ctaatagact ccggcaccac ggtgcttgta
     1921 ggcgattaca ttttcgccaa gtcggcggtg ctctccacca tgggcggcaa gccccgcgcc
     1981 actcgcatct tcgccgatac gctcgttaca atctgcgaag gcgagatcga gcagaagttc
     2041 tcaacccatc acgtctcccg ctcggtcgag cagtactaca agcgcatcta ctccaagacg
     2101 gcagtgctgt tcgcggccgc gggcgagctt ggggctgtgc tgtcggacgc ccccgaagac
     2161 caggtgcaga gccttcgcca ctacggcaag caggtgggca tggccttcca gatcgtagac
     2221 gacatactag acctgcgcgc caccacccaa cagcttggca aaccgaccgg cggcgacctg
     2281 cgccagggca ccgtcacgct ccctaccatg tactacctcg aaacggctga cgacgagggc
     2341 cgggaggtca tcacccgcgt aatcgagaac gacgagcgca ccgacggcct cgtggagagg
     2401 gcgattcggc tgataaacga gtcgggcgcg gtcgagcgag cgcatgcgga agcagtcaat
     2461 ttcgtggaag aagccaaggc cgccctcact cgcctgcctg acatccctat caagcagtcg
     2521 ctgatagacc tggccgattt cgtggtcagg cgcgacttct aacaaaccgg gcaagtccac
     2581 tactcggcgg ttgagacggc gtacgacctc tacagggtcc ccaaaggggg actcttctta
     2641 cttaatgcca cccacacata agtctgccta atcggttaca atacttgtgc gtactacgca
     2701 aactacgcac aaccacctca ccctggaagc tcgatggacg gcgttctaaa aggtctcagg
     2761 ccctacagcc cggacgattt accccaactg agacggctcc ttaccgagac gcaagcctgg
     2821 ccccccgttg ccacgcccgc cgacgaagac attctcacac gctggaagcg ccgtggggtc
     2881 atccctcaga acgatatcag cgtgctaccc ggaccccgcg gcgagttggt ggctttctcc
     2941 caggggtttc gactggcgca cggcgtctcc cgcttcagct tcgagattgg tgtccgcccc
     3001 agccaccggc agcagggcat tggcggcgct ctctaccgcc tggtggaagc cagggcgcgt
     3061 gacatgggcg tcgctaacat gacaagcccc gtgttcgtca cttcgggcgt ggcgcggcct
     3121 gagtgcagcg gcttcctcga acggcggggc ttccgggtgg aatcgagcta ttggcagatg
     3181 cgcgtggacg acctcgtccg ccaggcaccc gcccgctggc ccgaaggcat agagtgcagg
     3241 cccttcggag acccccaccg cgacgcaccc aggtgggcgc agctcatccg cgacaccttc
     3301 aacgaacacg ccaccgccga gggcatcctc cagcaacttt cggaggaggg cgtcctccgt
     3361 cacggctact tcttcgccgt cgaccgagcc acggggctgg aggtgggcac gacccgctcg
     3421 cgcatcgact aca
//
LOCUS       DASRWZ010000491         7650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_519946, whole genome shotgun sequence.
ACCESSION   DASRWZ010000491 DASRWZ010000000
VERSION     DASRWZ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519946"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..3332)
                     /locus_tag="VFH60_07675"
     CDS             complement(171..3332)
                     /locus_tag="VFH60_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HET6313697.1"
                     /translation="MDRRRLSARADMGSNVGPVRREEVAHPALDRAINLAALNWEVVA
                     FVGLMLVAAFTRLVDLGPRAFHHDESIHAFFSDHFLKTGNYTTTSGFDGGYDPTYHGP
                     FLYHATALMFYLFGTNDYNARLAPAIFGIILVGLCWLFRPFIGRVGALIATFLVIFSP
                     SINYYSRSLRHDVFALAGITLLVIAILWFMRTHQSRWVYLGAVGFSIAFASHELTYLM
                     AVVFAAFLLLALFLFRSFSERATAGLPAVVGDEDVNPVKSAWSALMARPWTLLGGALT
                     FLGIYAVLFTNMLIKPQLIFSGVLGPLYWLGQHGVNRGTQPEFYYMLLMPIYEPLALL
                     AGLGTAVWLITRLVRGKADERQSDDTDIVTDGPEDQYGVPYPSMQGMRGFGLAFIAVW
                     SFGALVVFSIAGERLPWLNMHIAMPFTLLASAGLAALITRVDWREARKGGAVFLGITI
                     VLFIIALFAMIANFNGNMPPATGQTAELQSFIRSIGLVIVVAGLLVLAGWLSYKMAPA
                     RAVAVVALTFAVLLGAYGVRAMSLAVYRHGDVPVEMLVYTQSAPDVPIVTAMVERLAR
                     DLTAFDGRNANDVTGGHTLAIFIDQNDAIEWPLDWYFRDQKGKQYFNYFANGQEIPDE
                     QKANKIAPNAPVILASEQTENTAYFQNFIKDKYTTNRYPLNWWFPEETYKVNGKGDIG
                     KAISWLSGNGMKYLLYRDPGMPLGSRNFYLHVRNDLAPQVGLGSVAGTPSAAVDDGKI
                     YSLFDLSQGASRGQFHLPRGIASDPAGNFYVADTANYRIQKFAPDGKFLATMGNGKGS
                     GDGQFNPINEGGVGTGVGGIAVDAQGNVYAADTWNHRIQKFGPDGKFLAKWGAFINLG
                     DPAAAADPQRDSKFYGPRGVAVGPDGNVYVTDTGNKRVLVFAPDGTFVSKIDSGMSPT
                     RVAPDYPFNQPGELNEPIGIAVDQQKNVYVADTNNQRIQKFDAGGKFVAQWPVPGLNW
                     EAGTYMEPFLAVDAAGNVYATAPTGAAVLKFSPTGQLIATQKSVGAITLKVPTGITVA
                     PNGSVVVVDTTGHGVVNMGQINP"
     gene            complement(3410..6883)
                     /locus_tag="VFH60_07680"
     CDS             complement(3410..6883)
                     /locus_tag="VFH60_07680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6313698.1"
                     /translation="MKTERHRDRVPRPNTDASGLVPSPDGTDSDPAESDGNGSQPDTN
                     AELSAVESSAGTDTGNGYASHEDADGPANGLTEGNGHTPEVESSRVVLTVPRRKRRVV
                     YAPAAPEAPEAPEAVAPPAATVSEMIAPAEEKVVAPPPLPGVALPPKAKRKTGAPPVQ
                     TQPVTPVVEEEAPVVEAATESGGRWRVNITVEGVLWFAFFALAIGTRLWDLSNRGIHH
                     DESLHAVYGNNLYSGSGYIHDPMMHGPLQFHLIAFMYWLFGTNEMTARMASAFCGIWV
                     VMSPFFLRRHMGRVPALLASFLLLVSPSVLYFSRMAREDAIFSAMEMIMIVGLWRFVS
                     NRRPADFFIFAAGLSLMFTIKETAFLTLAVLAVFFVLLFIVQSGYAIVAATAVYGLFM
                     GGLLMYVRANMQELDAQNQVIPGSGTIPVLPNIPNPGPSYDILAKFATDLFTHPLVVG
                     ALILTIAWIVSLVALFRRERTRLQEAAPAMPARYRTAGVATTRANGGTLQTRATRGNG
                     SVAARNAVVAQPAIDVAETPSVAATQAATDPEAASELWNPRRLDPRPGTILARYQPGS
                     MPHLLGALFARPSVLLIGFAIAAAIFTIFYTVFFTDVPRGLLSGLFGSLGYWMAQHGV
                     ERGSQPWYYYLLLIPLYEPIAVFFSLAGTIFFGWRGVRWLRRNRAESRYEHNPPRLGA
                     FNTDRPVPLATFQPFLAAFIVWWLFGATFIYSWAGEKMPWLMVHMVRPAILLASLFLG
                     ALLLSILARRRERLQAAAAYDERYVGYAPGGPALAAGGMMRSTLARLSQPGLQVAGAL
                     PGGGAAPLDLSRGQLMQMSRGPQRSRPSAHTTARSRSTRPESITVSARRPVRGRGAAV
                     AVMPRQEPPWVSWNRPNSNFPALSFLTLFVLFAMSWGMTMTFQIALNNNYNTWGLTWV
                     WPGLMLALVIAFMAWLGPSRALRYLAVGLFSVFFLYQVRSAVMLTYFQPDVPKEMAVY
                     VQTSPDVTRSMKELKDFSKATTGKNDVKVMYDNEVSWPYSWYFHDFKNAQYIGAAEPQ
                     PAADVPVMVFGYDNKNGNTELPKNYTSQRYAMRWWFPEDWYKKDLMSGLPAGETSKSA
                     LAASAGLLVVKLGDTFTKPDNQATLWKYLHFRETPMPLGSFDMLVYVRKDVAPFYHYL
                     QYQPNPSDDLP"
     gene            complement(7072..>7650)
                     /locus_tag="VFH60_07685"
     CDS             complement(7072..>7650)
                     /locus_tag="VFH60_07685"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313699.1"
                     /translation="LAEDEGDLEELKALVRLLSDRVAWQLRHERFQARCIYLKLRLLP
                     KQRVWTPEGSGFGRLITRQVTIPQATDSDHTICAAAYGLLEAAAKATGLGSGRELVRL
                     IGVGTTNLVRMGEVQPSMDMGEMGQAAAGGPGPEQSSWGWQSRAQERSRRLNTSVDAI
                     RDRFGFKSITLAAGVKRHIMEAPEDPLKSGNT"
BASE COUNT         1446 a         2426 c         2430 g         1348 t
ORIGIN      
        1 gggaacatca gggtagtctc cgtaggagac ttcgtcccga gcaagcccgt ggctttagcc
       61 acagggcgtc atgcagggcg tccgtggcag ggcacaccca gtgggctacg tgccaggcgg
      121 gacaccaggg aggggcacca caatgcgtgc cccactatct ttcgcgcctc ttacgggttg
      181 atttgcccca tattcaccac gccgtgccct gtggtgtcca ccaccacgac gctgccgttg
      241 ggagctactg tgatgcccgt tgggaccttc aatgtgatag cgcccacgct cttctgcgtc
      301 gcgatcagtt gccctgtggg gctgaacttg agcaccgccg ccccggtggg tgccgtggcg
      361 tagacgttgc ccgccgcgtc caccgcgagg aagggctcca tgtaagtgcc cgcctcccag
      421 ttgaggcccg gcaccggcca ctgagccacg aacttgccgc cggcatcgaa cttctggatg
      481 cgctggttgt tcgtatcggc cacgtacacg ttcttttgct ggtccaccgc gatgccgata
      541 ggctcgttca actcgcccgg ctggttgaac gggtagtcag gcgcgaccct ggtcggactc
      601 atgccggagt ctattttgct cacgaaggtg ccgtccgggg cgaagacgag cacgcgctta
      661 ttgcccgtgt cggttacgta cacgttgccg tccgggccga ccgccacccc tcgcgggccg
      721 tagaacttgc tatctcgctg cgggtcggcg gctgccgcgg ggtctccaag gttgatgaac
      781 gcaccccact tggcgaggaa cttgccgtcc ggcccgaact tctggatgcg gtggttccag
      841 gtgtcggccg cgtagacgtt accctgcgcg tccaccgcga taccgcccac gcccgtgccc
      901 acaccgcctt cgttgatggg gttgaactgg ccgtccccgc tacccttgcc gttgcccatc
      961 gttgccagga acttgccgtc gggtgcgaac ttctggatgc ggtagttagc cgtgtccgcc
     1021 acatagaagt tgcccgccgg gtcgctggca ataccacgcg gcaggtggaa ctggccgcga
     1081 ctagcgccct gcgacaggtc gaacaggctg taaatcttgc cgtcgtccac ggccgccgac
     1141 ggggtgccgg ctacgctgcc caggcccacc tgcggtgcta ggtcgttacg aacgtgcagg
     1201 tagaagttgc gcgagccgag cggcatgccg gggtcgcggt agagcaggta cttcatccca
     1261 ttgccgctca gccagcttat cgccttgccg atgtcaccct tgccgttgac cttgtacgtc
     1321 tcctccggga accaccagtt gagcgggtac ctgttggtcg tgtacttgtc cttgatgaag
     1381 ttctggaagt aggcggtgtt ctcggtctgc tcgctggcaa ggatcaccgg ggcgttggga
     1441 gctatcttgt tggccttctg ctcgtccggt atctcctgcc cgttcgcgaa gtagttgaag
     1501 tactgcttgc ccttctggtc gcggaagtac cagtcgaggg gccactcaat cgcgtcgttc
     1561 tggtcgatga agatggcgag ggtgtggccg ccggtcacgt cgttggcatt gcgcccgtcg
     1621 aaggccgtca agtcgcgggc cagccgctcc accatggccg tgacaatcgg cacgtcgggg
     1681 gccgactggg tgtagaccag catctccacc ggcacgtcac cgtgacggta caccgccagg
     1741 gacatcgcac ggacgccgta cgcgcccagc agcacagcga aggtaagcgc caccaccgcc
     1801 accgcgcggg cgggggccat cttgtacgaa agccagcccg caagcacgag caacccggcg
     1861 accacgatta ccagtccaat gctgcggatg aagctctgca gttccgcagt ctggcccgtg
     1921 gcagggggca tattgccgtt gaagttcgcg atcatggcga acagcgcgat gatgaacagc
     1981 actatggtga tgcccaggaa gacggcccca cctttgcggg cctcccgcca atccacgcgg
     2041 gtgatcaagg cggccagccc cgccgacgcc agcagcgtga agggcatggc tatgtgcatg
     2101 ttcagccagg gcagcctctc acccgctatc gaaaagacga cgagcgcccc aaacgaccag
     2161 acggcaatga acgccagccc aaaaccgcgc atgccctgca tgctgggata gggcacgccg
     2221 tactggtctt ccggcccgtc ggtgacgatg tcggtgtcgt ccgactgccg ctcgtcagcc
     2281 ttgccgcgca ccagcctggt aatcagccag accgcagtgc cgagacccgc cagcagcgcc
     2341 agaggctcgt agatgggcat caggagcatg tagtagaact caggctgcgt gccccggttc
     2401 accccgtgct gccccagcca gtaaagcggc ccaagcacgc ccgaaaagat aagctggggc
     2461 ttgatcaaca tgttggtgaa cagcaccgcg tagatgccaa ggaaggtaag cgccccgccc
     2521 aacaacgtcc agggccgcgc catcaaagcc gaccacgcgc tcttcaccgg gttcacgtcc
     2581 tcgtcgccca cgaccgccgg caacccggcg gtagcacgct cggagaacga cctgaagagg
     2641 aacagcgcca gcagcaggaa ggcggcgaac accaccgcca tcaggtacgt cagctcgtgg
     2701 cttgcgaaag ctatggagaa gcccaccgcc cccaggtata cccaacgcga ttggtgggtg
     2761 cgcatgaacc acaggatcgc gataacgagc agcgttatac ccgccagcgc gaacacgtcg
     2821 tgccgcagcg acctggagta atagttgatg ctgggcgaga agattaccag gaaagttgcg
     2881 atcagcgccc ccaccctgcc gatgaagggc cggaacagcc agcacagccc cacgaggatg
     2941 atgccgaaaa tggcgggggc gagacgggcg ttgtagtcgt tcgtgccgaa caggtagaac
     3001 atcaaagccg tcgcgtggta gaggaacggc ccgtggtaag tggggtcgta gccgccgtca
     3061 aaaccggagg tggtggtgta gttgccggtc ttgaggaagt ggtccgagaa gaaggcgtga
     3121 atcgattcgt cgtggtggaa ggcgcgaggc cccaggtcca cgagccgggt gaaagcggcc
     3181 accagcatca gacccacgaa agccacaacc tcccaattga gggcggcgag gttaatcgcc
     3241 cggtcgagcg ccgggtgtgc aacctcttcc cgcctcaccg ggccaacgtt cgatcccata
     3301 tccgcgcgtg cgcttagtct gcgtctgtcc ataatctccc ttgttcctga gcctaatcag
     3361 taccgagtac cgagtactga gtaatcctca atccgaaatt ccaaattcgt cagggcaggt
     3421 cgtcggatgg gttcggctgg tactgcaagt agtgatagaa aggtgctacg tcttttcgca
     3481 cgtagaccag catgtcgaag gagcctagcg gcatgggcgt ctctcggaag tgcaggtact
     3541 tccagagggt cgcctggttg tccggcttgg tgaacgtgtc gcccagcttg accaccagta
     3601 gacctgcgga cgcggccagg gccgacttcg aagtctcccc ggcgggaagc cccgacatga
     3661 ggtccttctt gtaccagtcc tccgggaacc accagcgcat cgcataccgc tgtgaagtgt
     3721 agttcttagg aagctccgtg ttgccgttct tgttgtcgta gccgaacacc atgacgggca
     3781 cgtcggcggc cggttgcggc tctgccgcgc caatatattg cgcgttcttg aagtcgtgga
     3841 agtaccagct atacggccag gacacttcat tgtcgtacat caccttcacg tcgttcttgc
     3901 ccgtcgtggc cttcgaaaaa tcctttaact ccttcatgct gcgggtaacg tcgggagagg
     3961 tctgcacgta cacggccatc tctttgggca cgtcgggttg gaagtaggtc agcatcaccg
     4021 ccgaacggac ctggtacagg aagaagaccg agaagaggcc caccgccagg tagcgcaagg
     4081 cgcgagaggg gccaagccac gccatgaacg cgatcaccag cgccagcatc aggccgggcc
     4141 acacccacgt cagcccccag gtattgtagt tgttgttcag cgcaatctgg aacgtcatcg
     4201 tcatgcccca cgacatcgcg aagagcacga acaacgtgag gaacgacagc gccgggaagt
     4261 tcgagtttgg cctgttccag cttacccacg gcggttcttg cctcggcatt acggccaccg
     4321 ccgcacccct gccccgcacc ggcctgcgtg ccgagacggt tatgctctcc ggtcgtgtgg
     4381 agcgcgagcg agccgtagta tgggctgagg gccgggaccg ctgtgggccg cggctcatct
     4441 gcataagctg cccgcggctc aggtcgagcg gggcagcgcc gccgccgggc agcgcgcccg
     4501 ccacctgtaa gccgggttgg gacagcctgg cgagggtaga gcgcatcatg ccgcctgccg
     4561 ccagggccgg gccgcccggc gcatagccga cgtaacgctc gtcgtacgcg gcggcggcct
     4621 gcaacctctc gcgcctgcgg gcaagtatcg acagcaggag cgcgcccagg aagagggaag
     4681 cgagcagtat ggccgggcgc accatgtgca ccatgagcca cggcatcttc tcgcccgccc
     4741 agctatagat gaacgtcgcc ccgaagagcc accacacgat gaaggcggca aggaaaggct
     4801 ggaaggtcgc caggggaacg ggcctgtcgg tgttgaacgc gccgaggcgg ggcgggttgt
     4861 gctcgtaccg cgactccgcc ctgttccgcc ggagccaacg cacgccgcgc cacccgaaga
     4921 atatcgtgcc cgccaggctg aagaagacgg ctataggctc gtacaggggt atcagcagta
     4981 aatagtagta ccagggctgc gaaccgcgct ctaccccgtg ctgggccatc cagtagccga
     5041 gcgagccgaa caagcccgat agcaggcccc tcggcacgtc ggtaaagaag accgtgtaaa
     5101 agatcgtaaa gatcgccgcc gcgatagcaa agccaatcag cagcaccgaa ggccgggcga
     5161 agagcgcacc gagcaggtgc ggcatcgacc caggctggta gcgtgccagt atcgtgcccg
     5221 gcctggggtc gagcctgcgc gggttccata gctcgctcgc agcttcgggg tcggtggccg
     5281 cctgggtagc agccacgctc ggcgtctcgg ccacgtcgat ggccggctgc gccactaccg
     5341 cgttgcgggc agcgaccgag ccgttgcccc gcgtcgcccg agtctgtaaa gtgccgccgt
     5401 tcgcgcgcgt tgtcgcaaca cctgccgtcc tgtaacgagc gggcatggcg ggagcggctt
     5461 cttggaggcg ggtgcgttct ctgcggaaca aagcgacaag cgacacaatc caggcaatcg
     5521 tgagaataag cgcgcccaca acgagggggt gcgtgaacag gtcggtcgcg aatttggcaa
     5581 gtatgtcgta gctggggccc ggattcggaa tgtttggcag cactggaatg gtgccgctgc
     5641 ccggtatgac ctggttttgc gcgtcaagct cctgcatgtt cgctctcacg tacatcagga
     5701 gcccgcccat gaacaggccg tagacggcgg tggcggccac tatcgcgtac cccgattgga
     5761 cgatgaacag caacacgaag aagacggcca gcacagccag cgtaaggaag gctgtctcct
     5821 ttatggtgaa catgagcgac agcccggcag caaaaatgaa gaagtcggcc ggccgcctgt
     5881 tggacacgaa acgccacagc cccactatca tgatcatctc catcgcggag aagatcgcgt
     5941 cctcgcgggc catacggctg aagtagagga cgctgggtga taccagcagc aggaacgagg
     6001 cgaggagcgc gggcaccctg cccatatggc ggcgcaggaa gaagggcgac atgacgaccc
     6061 agatgccgca gaaagccgaa gccatgcgcg cggtcatctc gttggtgccg aacagccagt
     6121 acatgaatgc gatgaggtgg aactgcaacg ggccgtgcat catcgggtcg tggatgtagc
     6181 cgctgccgct gtagaggttg ttgccgtaga cagcgtgcag gctctcgtcg tggtggatgc
     6241 cccggttgct caggtcccac aggcgcgtgc ctatggcgag cgcgaagaag gcgaaccaga
     6301 gcacgccctc gaccgtaatg ttgacccgcc acctgccgcc gctctcggtc gccgcttcca
     6361 caacaggggc ttcttcttcg accaccggcg tcacaggctg cgtctggacg gggggagcgc
     6421 cggtcttgcg cttggccttc ggcggcaggg ctacgcccgg tagcggcggc ggcgccacga
     6481 ccttctcttc ggccggagct atcatttcgc taaccgtggc ggcaggtgga gcaacagctt
     6541 ccggcgcttc cggcgcttcc ggtgctgccg gcgcgtacac aacccgacgc ttacgcctgg
     6601 ggaccgtcag cactaccctg gaggactcaa cctcaggcgt atgcccgtta ccttctgtga
     6661 ggccgttggc cgggccgtcg gcgtcttcgt gggaggcgta gccattgccc gtatcggtac
     6721 cggcgctact ctccacggcg cttaattcgg cattcgtatc tggctgcgag ccgttgccgt
     6781 cgctctcggc ggggtcgcta tcggtgccgt ctggggaggg gaccagcccg gaagcgtccg
     6841 tgttcgggcg tggcacccgg tcacgatggc gctcagtttt cataagactc cttggaaagc
     6901 aaaatgaggc gggcctgtgc ctctaactgc cgggaatttg taattggaag cacttgcagg
     6961 ccactattca atagggggaa tctcgccgcg ataatacgcc agttagattt gattgtaccc
     7021 taactaacaa acgtacgcaa gcaaataaat gttacatgga ttacggaaag cttacgtgtt
     7081 gccactcttc agcggatctt cgggagcctc catgatgtgc cgcttgacgc ccgccgccag
     7141 ggtgatagac ttgaacccga agcggtcccg tatcgcatcc acgctggtgt tcagccgccg
     7201 gctgcgctcc tgcgccctgg attgccaccc ccaggacgac tgctcaggcc ccggtccgcc
     7261 cgccgcagcc tgccccattt cacccatatc catgctgggc tgcacctccc ccatgcgcac
     7321 gaggttcgtg gtgcccacgc cgataagccg gaccaactcg cgcccgctgc ccagccccgt
     7381 agccttagca gccgcttcaa gcagaccata cgcggcagcg cagatcgtgt ggtccgaatc
     7441 ggtggcctgg gggatggtga cttgccgggt gatgagcctg ccgaagccgg agccttcggg
     7501 cgtccatacc cgctgcttgg gcagcagccg cagcttgagg tagatgcaac gcgcctggaa
     7561 gcgctcgtgc cgcaactgcc aggctacccg gtccgaaagc agccgcacga gggcctttag
     7621 ctcctcgagg tcgccctcgt cctcggccag
//
LOCUS       DASRWZ010000492         5229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_794030, whole genome shotgun sequence.
ACCESSION   DASRWZ010000492 DASRWZ010000000
VERSION     DASRWZ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794030"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..612)
                     /locus_tag="VFH60_07690"
     CDS             complement(10..612)
                     /locus_tag="VFH60_07690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HET6313700.1"
                     /translation="MDTIPRTAALIIIDVQKGFDEPYWGQRNNPEAEENIARLLEAWR
                     RTGRPIYHIQHQSTEPNSTLRPGYVGSEIKEIVRPLGPEPVLRKHVNSAFIGTDLEAR
                     LRDGSQDTVVVTGLTTDHCVSTTTRMAANLGFKTYVVADGTPTFERTGYDGRHYKAED
                     IHAMELTSLNGEFATIVDTAALLRLIEAEEPAPKIVAPGR"
     gene            complement(892..>5229)
                     /locus_tag="VFH60_07695"
     CDS             complement(892..>5229)
                     /locus_tag="VFH60_07695"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HET6313701.1"
                     /translation="RLAPGYYFLRATSVAPDGGVTAETGVVLMVGRTGLVAKNQGTDL
                     LVWATDLLTGRPVPDYPLRVQEQVSVEGQASTLAHQGRTGPDGVLRLKLQEDSRGVIA
                     WSDQPGDAAVVSTAWSYRVQSSDYNDENSRDYRDDESQTAIYTDRPIYRPGQVVYFSG
                     IYRHNDDASYSLPPAGTTLDLFVTDERHVLVYSTTLSLSALGTFNGQFELPASAPTGE
                     YRLAAYKRTSVYNELVDVSFQVEEYRKPDFEVNVTTREGVTAGQPVTATLSTSYYFGG
                     PLAHLTATLNVWDTPYRFHWADPQTGERYRFGEERDGKSEDTRSERSYERSTDGDGLL
                     VLDLGQDVDTSNGSRSLLVEGQVQDLSNQRVAKRDSLIVHQGLYYVGLREDTYIGQAG
                     QPFAYTLRTVAHDDKAATGGRIVPGVPLTVRVLRQEWSDSELRETEVATGSVTTDAEG
                     RARYAFTPDAAGAFRLVVEGTDSLGNKIRSSTRSWVSGPDAGSVRWRYSNDRSVDLVA
                     DKEQYNVGETARLLVTSPFTEATALLTVERGHLRRYSVVTLSGGAPVVEVRLEEGDLP
                     NVHIGITLLGRGPAPAFAPPGWVDSVQLREGSTNLHVSAESKRVRVSIEPQGQGGLKP
                     GGTTNVLVRTTGYDGKPTPAELSLAVVDKPIFALADDNSADLFDMFWYERPSQVETSS
                     SYRSGESHIVSRGGGVDILPSTGGMPRTGGSGGEEPARVRSDFRDTAFWRAAVTTGAN
                     GTALVAVRWPDNLTTWRLTARAVTADTSLGSGVSELTITQPVLVRPVTPRFLVVGDHP
                     RMQAVVHNNTSSTLQLQASLVVSGALTLDAKAAPSRRLILSPGTQATVSWDTRVIAGD
                     TGGMRYWVRTVGRVGGAYLEDAVAMQLPVKEFAAPEMVFVSGEVTGTRASESLYLPYS
                     EDPLAGELTVQVVPSLAAATAGGVKYLVEFEHACTEQTTSRYLPLVVLERAYKSQGQK
                     TPYSDQLPEIMQRAFNRLRELQHGDGAWGWWEFDDTRWWQTAYVVHGLIEARNSGYNV
                     PEELLLPGLASLRTFMRDNEPNGLDPTSLLNVRAYTLYLLTQAWKPDDTPGKVGLTEH
                     AKQLLGRVSEMSVHARSWLAMSLVRLGLSQESALVLDGVVAAARQDGTLAYWGEAQPD
                     YAGMGTDSRATAIALDALITIRPNDPLIPKVVRWLMSISRDGHWDSTQDTSFILIAIA
                     HYLEASKELSANYTWQASAFGTSLGGGVVGPTNLTQTQTLKVPLSKLPRNSRGSLEFT
                     RSSPAGKMYYQMTLRYFAPGEPIEARSKGFSVARSYYTLEGTEPVRDVRAGDVVKVRL
                     TITAPRTSYYVVVTDPLPGGLEGVNGSLKTTSFSERPPNARGTRLQDEGDSSGYRYRY
                     WGAFSNVEMRDDRTLLYATRLSPGTYVYEYFARATTPGVYMSLPASAEQMYQPQVYGH
                     SDGGVFTVK"
BASE COUNT          821 a         1697 c         1713 g          998 t
ORIGIN      
        1 tggttggtgc tatcgtccag gcgctacaat tttcggtgcc ggctcttctg cctctattag
       61 cctgagcaag gccgcggtgt ccacgatggt agcgaactcg ccgttgaggc tggtaagctc
      121 catcgcgtgg atgtcctcgg ccttgtagtg cctgccgtcg tagccggtgc gctcgaaggt
      181 gggggtgccg tcggccacaa cgtacgtctt gaagcccagg ttggcggcca tcctggtggt
      241 cgtggagacg cagtggtcgg tggtcaggcc ggtgacgacg accgtgtcct ggctgccgtc
      301 cctgagcctg gcttcgaggt cggtgccgat aaaggcgctg ttgacgtgct tccgcaacac
      361 cggctccggt ccaagcggcc tcactatctc cttgatttcg ctccccacgt agccggggcg
      421 caatgtggag ttcggctcgg tggactggtg ctggatatga tagatgggcc tgcctgtcct
      481 tcgccatgcc tcgagcagcc gggcgatgtt ctcctcggcc tccgggttgt tcctctggcc
      541 ccagtacggc tcgtcaaagc ccttctgcac gtcgatgata atcagtgccg ccgtccgcgg
      601 aatggtgtcc attgtcttct cctggttacg ttcatttagc ttgtccttac aacaattctc
      661 cctgggagga ctataaaaga gaggggagaa gagggagaac tgggggacac ccccggtccc
      721 cccgtcaggg gagcacccct gcactccctg cgggagaatt gagggatacc ccacgccccc
      781 gtgaggagag taccctcgct acccccttgt gagggagaga accggggaac acctccagct
      841 ctagcctggg gagtacctct gcaccccttc tcgcacagtc tggcttccgc tctatttgac
      901 ggtaaagaca cccccgtcgc tatgcccgta tacctggggc tggtacatct gctcggcgct
      961 ggcgggcagc gacatgtaga cgccgggtgt ggtggctcgg gcgaagtatt cgtagacgta
     1021 ggtgcccggc gacagcctgg tggcatagag caaggtgcgg tcgtcgcgca tctcgacgtt
     1081 gctgaacgct ccccagtagc ggtacctgta gccggaggaa tcgccttcat cttgaagtct
     1141 ggtgccacgg gcattgggcg ggcgctcgct gaaggaggtc gttttgaggg agccgttcac
     1201 cccttcgagg ccgccgggta gcgggtcggt gaccaccacg tagtacgagg tgcggggtgc
     1261 ggtaatcgta aggcgcacct tcaccacgtc gcccgcccgc acgtctctga ccggctcggt
     1321 cccctcgagg gtatagtagc tgcgcgccac cgagaagcct ttgctgcggg cctcaatcgg
     1381 ctcgccggga gcaaagtagc gcaaggtcat ctggtagtac atcttgccgg caggcgacga
     1441 gcgggtaaac tccaggctgc cccggctgtt cctcggcagc ttcgataagg gcacctttag
     1501 cgtctgcgtt tgggtgaggt tggttggccc caccactccc ccgcccaggc ttgtgccgaa
     1561 cgcgcttgcc tgccaggtgt agttggcgct cagttccttg ctcgcctcca ggtagtgagc
     1621 tatggcgata aggatgaagg aggtatcttg cgtgctgtcc cagtgcccgt ctcgcgagat
     1681 agacatcagc cagcgcacta ccttcgggat gagagggtcg ttgggcctga tggtgataag
     1741 ggcatcgagg gctatcgcgg tggcgcggct gtcggtgccc atgcccgcgt agtcgggctg
     1801 cgcttcgccc cagtaggcga gcgtgccgtc ctgccgcgcc gccgccacca caccgtctag
     1861 caccagggcg ctctcctgcg acagccccag cctcaccagc gacatggcaa gccaggagcg
     1921 ggcgtgcacg ctcatttcag agactcgccc caacagttgc ttcgcgtgct ccgtcagccc
     1981 cacctttcca ggcgtatcgt ccggcttcca ggcttgagta agcaggtaga gcgtataagc
     2041 tctcacgttc agtaacgaag tcgggtcgag gccgtttggt tcattgtcgc gcatgaaggt
     2101 gcgcaggctg gcgaggccgg gcagcagcaa ttcctccggc acgttgtagc ccgagttgcg
     2161 cgcctcgata aggccgtgca ccacataggc cgtctgccac cagcgggtat cgtcgaactc
     2221 ccaccagccc cacgccccgt cgccatgctg aagttcccgc agccggttga aggcgcgctg
     2281 catgatttcg ggaagctggt cgctgtaggg ggtcttctgg ccctggctct tgtaggcacg
     2341 ttccaggacc acgaggggca ggtagcggct ggtggtctgc tcggtgcagg catgctcgaa
     2401 ctccaccagg tatttcacgc cgcccgccgt ggccgccgcc agcgacggga ccacctgtac
     2461 ggtcaactcg cccgccagcg ggtcttcgct gtacggcagg taaaggctct cagaggcgcg
     2521 cgtgcccgtg acctccccgc tcacgaagac catttcgggc gcggcaaact ccttcacggg
     2581 caactgcatg gctaccgcgt cttccaggta ggcaccgccc accctgccta ccgtgcgcac
     2641 ccaatagcgc atgcccccgg tatcgcccgc gatcactctg gtgtcccagg agacggtggc
     2701 ctgagtgccc ggcgacagga tgaggcggcg cgacggggcg gctttggcgt cgagagttag
     2761 ggctccgctc accaccaggc tggcctgtag ctggagggta gaggaggtgt tgttgtggac
     2821 taccgcctgc atgcgggggt ggtcgcccac gaccaggaac cggggtgtta cggggcgcac
     2881 caggacgggc tgggtgatgg tcagttcgct tacgccgctg ccgaggcttg tgtccgcggt
     2941 gacggcccgc gccgtcagcc gccaggtggt gaggttgtcg ggccagcgca ccgccaccag
     3001 ggccgtaccg ttcgccccgg tggtcacggc cgcgcgccag aaggcagtgt cgcggaagtc
     3061 ggagcgtacg cgtgcaggct cttcgccccc gctgccaccc gtccggggca taccacccgt
     3121 gctaggcaat atgtccaccc cacccccacg gcttacgatg tggctctcgc cggagcggta
     3181 gctgctggag gtctctacct gcgagggacg ctcgtaccag aacatgtcga agaggtccgc
     3241 cgaattatcg tcagcgaggg cgaagatcgg cttgtctacg accgctaggg aaagctcggc
     3301 gggggtgggc ttgccgtcgt acccggtggt gcgcaccagc acgttcgtgg tgccgcccgg
     3361 cttcaagccg ccctggccct gcggctcgat gctcacgcgc acccgcttgc tctcggcgct
     3421 tacgtgcaag ttcgtggagc cttcgcgcag ttgtacgcta tccacccagc cgggcggggc
     3481 gaacgcgggg gcagggccgc gccccagcag cgtgataccg atatgcacgt tcggtaggtc
     3541 gccttcctcc aaccgcacct cgacaaccgg cgcgcccccg ctcagggtga cgacgctgta
     3601 ccggcgcagg tgcccccgct ccacggtgag cagggctgtg gcctcggtga agggggaggt
     3661 cacgaggagg cgggccgtct cgcccacgtt gtactgctct ttgtcggcca ccaggtccac
     3721 gctccggtcg tttgagtaac gccaccggac cgagccggcg tcggggccgg atacccagct
     3781 ccgagtgctg cttcgtatct tattccccag gctgtcggtc ccttccacga cgaggcggaa
     3841 agcgcccgcg gcatcgggcg tgaaggcgta gcgggcgcgg ccctccgcat cggtcgttac
     3901 gctgcccgtg gctacctccg tctcacgcaa ttcggagtcg gaccattcct ggcgcaggac
     3961 gcgtacggtc agcggcacgc cgggcacgat gcgccccccg gtggccgctt tgtcgtcgtg
     4021 ggccacggtc cgcaaggtat acgcgaacgg ctggcctgcc tgcccgatgt acgtatcctc
     4081 gcgcaggccc acgtagtaga ggccctggtg cacgatgagg ctgtcgcgct tcgccacacg
     4141 ctggttgctc aggtcctgga cctgcccctc tacgagcagg ctgcggctgc cattgctcgt
     4201 gtcaacatcc tggcccaggt cgagcaccaa cagcccatcg ccgtccgtgc tccgttcgta
     4261 gctccgctcc gaccttgtgt cctccgactt gccgtcgcgt tcctctccga accggtaccg
     4321 ctcgccggtc tgcggatcgg cccaatggaa tctatagggg gtgtcccaga cgttcagcgt
     4381 ggcggtgagg tgggcgagcg gaccgccgaa gtagtagctc gtgctcaggg tggcggtaac
     4441 cggctggccg gcggtcacac cctcgcgagt tgtaacgttt acctcgaagt ccggcttgcg
     4501 gtactcctcc acctggaaac tcacgtccac aagctcgttg tacaccgagg tgcgcttgta
     4561 cgcggccagc ctgtactccc cggtgggcgc gctcgcgggt agctcgaact gcccgttgaa
     4621 cgtgccgaga gcggaaagtg agagagtagt tgaatagact aggacgtggc gctcatcggt
     4681 gacaaaaagg tccagggtag tgccggcggg cggcagcgag tagctggcgt catcgttgtg
     4741 gcggtagatg ccgctgaagt agaccacctg gccggggcgg tatatcggcc tgtcagtata
     4801 gatggcagtc tggctttcgt cgtcccggta atccctgctg ttttcatcat tgtagtcgga
     4861 agactgcacc cggtagctcc acgcggtgct taccacggcg gcatcgcccg gctggtcgct
     4921 ccacgcgatt accccgcgag aatcctcctg caacttcagc cgcaacacgc cgtcggggcc
     4981 ggtgcggccc tgatgcgcaa gcgtagaggc ctgcccctca accgacactt gctcctgtac
     5041 ccgcaacggg taatcaggca cgggccgccc cgtcagcaag tcggtcgccc agaccagcag
     5101 atcggtgccc tggtttttgg ctaccaggcc ggtgcggccc accatcagca ccacgccggt
     5161 ctcggcagtc acgccgccat cgggcgcgac actcgtggcc cgcaggaagt agtagccggg
     5221 cgcgaggcg
//
LOCUS       DASRWZ010000493         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1341898, whole genome shotgun sequence.
ACCESSION   DASRWZ010000493 DASRWZ010000000
VERSION     DASRWZ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341898"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1730
                     /locus_tag="VFH60_07700"
     CDS             303..1730
                     /locus_tag="VFH60_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET6313702.1"
                     /translation="MSTGSVNDLLASALPIVLALVMGVLGAIRGFWREAIVSASIVLG
                     ASIVQQWASIWATDLYEMYTGASREWQQVALSMLLLSLIVLVLGYGLGTLLGRGRTSV
                     GSRTGGMLLGLVNGLAIGGWLLRYIFTGLDGAQPSSPLYQNQLTQAFMIGAGWFSVAL
                     AVVGALVALIAPFRKPQVEVVETAPAPATAPVWTPPPPAPAYSAGTTSTTGTATAYGA
                     PPAYSPPAYGAPPAGRIPGTVGAATPYDRTVANPGLYPTPAPPAPYDTSRTQAYTGMD
                     APVPARDPYNPYPPDTATRTFAPVTREAGAAPPTAGLADSSWAAPAREAPVYRSALGA
                     NTEIERTTALPPDGQPDEAALPPASGPEALRPSSDWLAASTVSSSVPSTPVEDERVDV
                     EESQDAESAPDPLAELREPETTAVAVADEAKETDDTDDTSGAPAAQAAGTTYKPTDQT
                     CDTCGAPILNNAQFCAECGTRVLKA"
     gene            1787..>2357
                     /locus_tag="VFH60_07705"
     CDS             1787..>2357
                     /locus_tag="VFH60_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6313703.1"
                     /translation="MQTNRWLQTLIVLLVIIASAWLAGQVWTFLMQFSSILLLFFLAW
                     LLAFVLSPIARALQRRGVPKTLAVGIVYLALAVLAVLAVWWLVPPITAQVGQLQSGIE
                     SGSYMTTFNQFVQDLQKALTGLGLESTDLQRIYNEISAQAQNVLLNVVSNTFTMLQGV
                     ATFALQFILVLILSFYFMNDSDRLFNGIVE"
BASE COUNT          424 a          766 c          715 g          452 t
ORIGIN      
        1 gacgagattt ctcactacgt tcgaaatgac aaagtggggc ctgggtgcaa gtgttggacc
       61 atctggtgaa aacgatggat tctagagggt agtctctgaa ggagactttg ctacttccca
      121 gcccgtgact ttagccacgg ggcgtcgtca ggcccccgcg tggcgaacca aggcaccctg
      181 tacacgcaca aatacactcc cacagcccgg tgacacagcc ttcaacagcg tgctataatg
      241 ttgcgacggc gcaatttctg tctattggtc ccgtatatca tgacgtagtt ggggtaactt
      301 atatgtcaac cggctctgtc aacgatcttc tggcttcggc tctgccgata gtgctcgcgt
      361 tggtaatggg tgtcctcggc gcaatccgag gcttctggcg cgaggcgatt gtatcggctt
      421 caattgtgct tggcgcgtcc atcgtccagc agtgggcctc catatgggcc accgacctgt
      481 acgagatgta cacgggggcg tcgcgtgagt ggcagcaagt cgcgctgagc atgttgctgc
      541 tctctcttat tgtgctggtg ctcggctacg ggctgggcac gttgctgggc agagggcgta
      601 cctcagtagg ctcgcgcacc ggcgggatgt tgctggggct ggtgaacggc ctggccatcg
      661 gcggctggct gttgcgctac attttcaccg gcctggatgg cgcgcagcct tcaagcccgc
      721 tgtaccagaa ccagctcacc caggcattca tgatcggggc cgggtggttc tccgtggccc
      781 tggccgtcgt tggtgccctc gtggcgctaa tcgccccgtt ccgcaagccg caggtcgagg
      841 tcgtggaaac ggcacccgca cccgcaacag cgcccgtttg gacccctcct ccgcccgcgc
      901 cggcttactc cgccggtacg accagcacga ccggcaccgc caccgcctac ggtgctcccc
      961 cggcgtatag ccccccggcg tatggcgctc ccccggccgg aaggatacca ggtacggtcg
     1021 gagctgccac accctacgac cgcacggtag ccaaccccgg cctatatccg acgcccgccc
     1081 caccggcacc ttacgacacg tccaggaccc aggcatacac cggcatggac gctcccgtgc
     1141 ccgcgcgcga cccctacaac ccctacccgc cggatacggc gacccgcacc ttcgctcccg
     1201 tcacgcgtga ggccggggcc gccccgccca ccgccgggtt agcggacagc agttgggctg
     1261 ccccggccag ggaagccccc gtgtacaggt cggcactcgg cgcgaatacc gagatcgagc
     1321 gcaccacggc cttgccaccc gatggccagc ccgatgaggc tgcgctccct cccgcttccg
     1381 gccctgaggc gttgcggccc tcgtcggact ggctcgctgc ctccaccgtg tcgtcctcgg
     1441 tgccaagcac cccggtagag gacgagaggg tagacgtaga agagtcgcag gacgcggaga
     1501 gtgcgccgga ccccttagcc gaattacgcg agcctgaaac gacggctgtc gcagtagcgg
     1561 acgaagcgaa agagacggac gacacggacg acacgagtgg cgcgcctgct gcccaagccg
     1621 ctgggacgac gtacaagcct accgatcaga cttgtgacac ttgcggggcg ccgatattga
     1681 acaacgcgca gttctgtgcg gaatgtggca cacgcgtcct gaaggcgtga ggctccttca
     1741 aaggctgccc gtaactttag gagcattggg gaacgagagg aagtaaatgc agactaatcg
     1801 ctggttacag acactcatag tgctgctggt gattatcgca tctgcctggc tagcagggca
     1861 ggtgtggact ttcctcatgc agttctccag catcctccta ctcttcttcc tcgcctggct
     1921 gctcgccttc gtgctcagtc ccatagcgag ggcgttgcag cggcggggcg tgcccaagac
     1981 gttagccgtc ggcatcgtgt acctggcgct ggcggtgctg gcggtgctcg ccgtttggtg
     2041 gttagtgccg ccaatcacgg cgcaggttgg ccagttgcaa tcgggcatcg agagtggcag
     2101 ctacatgacc actttcaacc agttcgtcca ggacctccag aaagcgctta ccggcctggg
     2161 cctcgaaagc acggacctcc agcgtatata caacgaaatc tccgcgcagg cgcagaacgt
     2221 gctgctgaac gtcgtctcga acacgttcac catgttgcag ggcgtggcaa cctttgccct
     2281 gcaattcatc ctggtgctga tcctgtcctt ctacttcatg aacgacagcg accgcttgtt
     2341 caacggcatc gtggagc
//
LOCUS       DASRWZ010000494         6407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1205527, whole genome shotgun sequence.
ACCESSION   DASRWZ010000494 DASRWZ010000000
VERSION     DASRWZ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205527"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            438..2510
                     /locus_tag="VFH60_07710"
     CDS             438..2510
                     /locus_tag="VFH60_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HET6313704.1"
                     /translation="MSGIKSWFSGAPRRVGLRLGLALSVMLMTFAGYGGVPAVPAHAA
                     AHEVAMREFKFVQPQLRVAVGDSVSWVNYDTESHNIAILEGPELNVSPEQRKGEKWAM
                     TFTKPGRYHYYCEFHPWMVADVIVGGNESTVSTERAAQTFRETGKSVRGKFLQYWQKN
                     GGLAQQGFPISEEMVERSETNGQYYTVQYFERAVFELHPEEKPPYDVLLSLLGTFEYQ
                     RKYPNGAPGQQPNTSAGSVLFKETGKRVGGRFLQYWQQNGGLAQQGLPISEEFNEKSE
                     LDGKTYRVQYFERAVFEMHPENKPPYDVLLSQLGKFRHDKVVKAGEPNAMGIRQIGIS
                     GSPQHYPLLEGPHAAPGLNVYIYDQDPAPVVQWMDDLGTKWALHQLSWYQIETEPGVY
                     RWEKIDRAVDALYKAGIKVVLHPVHSPPWEWTDVGKVGYPKNIDNFKKFMTAAAQRYK
                     GKVAAYQIWNEPNLAIESGKYVVAARYAEVLKAGYTSVKAVDPAAIIVTAALTPTGVN
                     DPNRAVDDVIFLRRLYAYNNGELKGYFDVLGAHPGSNANPPDTLHPEKPGPGPGWNTN
                     ASFYFRRIEQIRQVMVENGDAGKQMWLTEFGWASTETPAPGFEYAAQNSEQEQADYIG
                     KAFRMGRDNYSWMGPMLLFQLNFALPSVAPEPTDERIAWGILRRDGSKRPAYFAVQQY
                     AREYNGGR"
     gene            2910..4169
                     /locus_tag="VFH60_07715"
     CDS             2910..4169
                     /locus_tag="VFH60_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313705.1"
                     /translation="MAGGGGGEVPTSTAVASGPIDLPGADDQLATVEAIQNSQATAVT
                     VREAIPYPIHTNEATVVPVTSVFTDAWGSLAVATVVPADLDMDHLFVLYAVDPEARFV
                     VGVIVPRVLESKEPARLVTVGAGDGRITEIASYVGPSPGELALDGVFVYGADTDGEWV
                     VWNEASRVRAHNLASGETRLLDEMPQDGPFVSFEAPQVDQGVAVWNEKRRNASPYSSA
                     RVVPAAVRWADLASGEVSTLSDSGAYPAISWPVAAWLEYPASGAGVNDYSIDLMGRVM
                     LRNLETGESWDLPDLPALAGVALDGDSLFFTSVVGQGFLTNLHGDSPRLVAPFFSSPY
                     RQMSLNERLVTWRDQPWSPVYDRALDRLVFLAARPFGFPKIRVSNGQALAWEDVVNMV
                     ELEGKQAGYVIPADPVVYLLNTADLPK"
     gene            complement(4405..6291)
                     /locus_tag="VFH60_07720"
     CDS             complement(4405..6291)
                     /locus_tag="VFH60_07720"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HET6313706.1"
                     /translation="MNSTSPRIRKYKALAIALLLAIGLGIAAPTRPLATAHAQAQGRF
                     YPQTGTTLAPEFLPFYDGKGGVPIFGYPLTEPETEGGVKVQYLERARIEYHPQNRNTP
                     YEVQLGLLGSILTAGRQFPTVPQPNAPSGPDTQYFPETRHTLSGPFLRYWRGNGGLAL
                     FGYPISEPLQEGGLTVQYFERNRFELLPEVTGTPYEVQLGLLGRDLLAQRVQVRETTV
                     SIPTYAYEQAFYTPANDPISPYPRLDFDKVGPPSPRTYRLLVLESRYIKLTMLPQLGG
                     RLYEAIYKPTGHNELYRNPVIKPSRFGERGWWLASGGMEWAAPTQEHGMMEYLPWDAS
                     VQRNSDGGATVTLSANDKLTGLTVVSAISLSPEDAAYTTSSRLENRTTRQAIGQLWSN
                     AMLAPGGTNSVSPRTRFIVPARTLVVHSTNDSSLPSEHGIIDWPNHNAKDLAGMDTWN
                     GWLGAFALPAPDRGAFAAVYNPDADEGLVKTFKNQEMPGLKIFGFGPSLNPANYTDDN
                     SSYAELWGGIVPTFWDNALFPPGSALGWTERWQPVARTGGISLASAWGTVSTQRGTLN
                     ILPTRRTEGATLTLRAPDGTTTRTTFNAYPDRPTTLPAPPTLSEIEITAPNGATLLKG
                     APIP"
BASE COUNT         1190 a         1622 c         2339 g         1256 t
ORIGIN      
        1 gtcagcccgt ggctttagcc acaggggtat gcggcttacc tgctgaatgg catcacttcc
       61 tatgcaggtg tggccctttt cttgcaggcg acaatggtgc tatggttgct gaagaaaggg
      121 gttgtttggg tggtgtagga cgagattctt cgctgcgctc agaatgacaa agggggagcg
      181 tggtgctgta gaaggggctg tcaaacactg gtggctgatc ccttcggcta cgcttaggac
      241 tacagagggg gagtacggtc ctgtaggttg gttttggggt agagggtagg cagggatggt
      301 tgcaggatgt tctacgtgta tcctgggtgc gtatatatga gctgagccgt gcctccgcat
      361 aggctttttg ttatgaggcg ggggtgtggc aggttggata gccttcgatt ctagctagcg
      421 ttttacatgg agggtccatg agcggtatca agagttggtt ttctggagcg ccccggcgtg
      481 tgggattgcg cttggggctt gcgttgagcg tcatgttgat gacgttcgcg gggtatgggg
      541 gcgtgccggc cgtcccggcg catgcggccg ctcatgaagt ggcgatgcgc gagttcaagt
      601 tcgtgcagcc ccagttgcgc gtggcggtgg gtgatagcgt aagctgggtg aactacgaca
      661 cggagagcca caatatcgcc attctggaag ggccggagtt gaacgtctcg ccggagcagc
      721 gcaagggcga gaagtgggcg atgacgttca ccaagccggg gcggtaccac tactactgcg
      781 agttccaccc gtggatggtg gccgacgtga tcgtaggcgg caacgagagc accgtctcga
      841 cggaaagggc ggcccagacc ttcagggaga cgggcaagtc ggtgcggggc aagttcctgc
      901 aatactggca gaagaacggc gggctggcgc agcagggctt ccctatctcc gaggagatgg
      961 tggagcgtag cgagacgaac gggcagtatt ataccgtgca gtacttcgag cgcgcggtgt
     1021 tcgagcttca cccggaggag aagccgccgt acgacgtgct gctgtcgctg ttgggcacgt
     1081 tcgagtacca gcgcaagtac cccaacggcg cgcccggaca gcagccgaac accagcgcag
     1141 ggtcggtgct gttcaaggag accggcaagc gggtaggcgg caggttcttg cagtactggc
     1201 agcagaacgg cggcctggcg cagcagggct tacccatatc ggaggagttc aacgagaaga
     1261 gtgagctgga cggcaagact taccgtgtgc agtacttcga gcgcgcggtg ttcgagatgc
     1321 accctgagaa caagccgcct tatgacgtgc tgttgtcgca gcttggcaag ttccggcacg
     1381 acaaggtagt gaaggctggc gagcctaacg cgatgggcat ccggcagata ggcatttcgg
     1441 gcagcccgca gcactacccg ctgctggagg ggccgcacgc cgcgccggga ctgaacgtgt
     1501 atatctacga ccaggacccc gcgcccgtgg tgcagtggat ggacgacctg ggcacgaagt
     1561 gggcgttgca ccagctttcg tggtaccaga tcgagacgga gccgggcgtt tatagatggg
     1621 agaagatcga ccgggcggtg gacgcgctgt acaaggcagg catcaaggtg gtgctgcacc
     1681 cggtgcactc gccgccgtgg gagtggaccg acgtgggcaa ggtcggctac cccaagaaca
     1741 tagacaactt caagaagttt atgacggcgg cggcccagcg ctataagggc aaggtggcgg
     1801 cttaccaaat ctggaacgag cctaacctgg caatcgagtc gggcaagtac gtggtggcgg
     1861 cgcgctacgc cgaggtgctg aaagcgggat atacgtcggt caaggcggtg gaccctgcgg
     1921 cgattatcgt gacggcggcc ctcaccccga cgggtgtgaa cgatcctaac agggctgtgg
     1981 acgacgttat cttcctgcgc agactgtacg cttacaacaa cggggagcta aagggctact
     2041 tcgacgtgct gggggcgcac cctggctcga acgccaaccc gcccgatact ctgcacccgg
     2101 agaagccggg gccggggccg ggctggaata ccaatgcgag cttctacttc cggcgcatcg
     2161 agcagatacg gcaggtgatg gtggagaacg gcgacgcggg caagcagatg tggctcaccg
     2221 agttcggctg ggcgagcacc gagacccccg cgcccggctt cgagtacgcg gcgcagaaca
     2281 gcgagcagga gcaggccgat tacataggca aggcgttccg catggggcgc gacaactact
     2341 cgtggatggg gccgatgctg ctgttccaac tcaacttcgc cttaccgagc gtggcacccg
     2401 agccgaccga cgagcgtata gcctggggca tcctgcggcg ggacggcagc aagcggcccg
     2461 cctacttcgc ggtgcagcaa tacgcgcggg agtacaacgg cggaagataa ccaacgagag
     2521 acgaaaacgc aaagacgcaa aggacgcaaa gaacgcgcaa agaggatgta ggtttagtgg
     2581 ataaaaggac cagggtatct agaggagtac cctggtcctt tttgctgctt gttggtgcac
     2641 gtgtatgggt ggtgagcggt agtttagcgt atggcacggt aagtggtaaa atactggtga
     2701 gtagtatagt tgccagggtt tgattggaga gtgcagtagt gggtattcag cttcgtgggc
     2761 ggggattgtt aattgtctct cttttgttgc tgtgtgctac gggtgtgctc gttggcggct
     2821 gcaatatggt ccagactcct gagggcttgc cgacgccgac gccgtatagc cttgaagtgc
     2881 ctcctgtgcc cagcgcagta gcgagcacta tggcaggggg aggcgggggc gaggtgccga
     2941 cctccacagc cgttgcttcc ggcccgattg atctaccagg cgcagacgat cagttggcga
     3001 cggtcgaagc catacagaat tcgcaggcca cggcggtgac ggtgcgcgag gcgatcccct
     3061 atcctatcca tactaatgaa gctaccgtgg tgcccgtaac cagtgtgttc accgacgcct
     3121 ggggctcgct tgcggtagcc acggtagtgc cggcagacct ggatatggac cacctgttcg
     3181 tactgtacgc cgtagaccct gaggcgcgtt tcgtagtggg cgttatagtg cccagggtgc
     3241 tggagagcaa agaaccggcc cggttggtca cggtgggcgc gggcgatggg cgtattacgg
     3301 agattgccag ctatgtgggg ccttcgcccg gtgagttggc tttagatgga gtgttcgtct
     3361 atggcgccga tacagatggc gaatgggtag tctggaacga ggcgtcgagg gtgcgggcgc
     3421 acaacctggc ttcgggcgaa actcgactgc tcgacgaaat gccgcaggac ggcccctttg
     3481 tttctttcga ggctccgcag gtggaccagg gagtagccgt atggaacgaa aagcgtcgca
     3541 acgccagccc ctatagttcc gcacgcgtgg tgcccgccgc cgtaaggtgg gcagacctgg
     3601 ctagcggcga agtctcaacg ttgagtgatt ctggggctta cccggccata tcgtggcccg
     3661 tagctgcgtg gctggaatac ccggctagcg gtgcgggtgt taacgactac tcaattgacc
     3721 tcatgggcag ggtgatgctt cgcaacctgg agaccgggga gagttgggac ctgccggacc
     3781 tgcccgcgct ggcgggggta gcgcttgatg gcgacagctt gttcttcacg agcgttgttg
     3841 gacagggctt cctcaccaac ctccacggtg acagtcctcg cctggtagct ccgttctttt
     3901 caagccctta ccggcagatg tccctcaacg agcgtttggt tacctggcgc gaccaaccgt
     3961 ggtcaccagt gtacgacagg gcgctggacc gcctggtatt cctggcggcg cgtcccttcg
     4021 gtttccccaa aatacgagta tccaatgggc aagcgcttgc ctgggaggac gtcgtgaaca
     4081 tggtggaact ggaaggcaag caggcaggct acgtaatccc ggctgacccg gttgtatacc
     4141 tgctgaatac tgcggacttg ccaaaatagc agctttctag acgaaaccgc caagacgcca
     4201 caaacgcgca aagaggatga ttatttttca ccgcagagac gcagaggacg cagagggtac
     4261 gcagagatga attgagggtg tacaagacat gcggaccagg gtacataatg tcgtaccctg
     4321 gtccttttta taccctctaa actttactta cttggcgtgg tccttggcgt cttggcgtct
     4381 tggcgtttcc gtctcccagt ggtgctaggg tatgggagcg cctttgagga gggtggcgcc
     4441 gttgggggcg gtgatttcga tttcggagag tgtgggaggt gcggggaggg tggtagggcg
     4501 gtcggggtag gcgttgaagg tggttcgggt ggtggtgccg tcgggggcgc gcagggtgag
     4561 ggtagcgcct tcagtgcggc gggtgggtag gatgttgagg gtgccacgct gggtggagac
     4621 ggtgccccag gctgaggcta agctgatgcc gccggttctg gcgacgggct gccagcgttc
     4681 ggtccagccg agggcgctgc cgggtgggaa gagggcgttg tcccagaagg tgggcacgat
     4741 gccgccccat agctcggcgt aggacgagtt gtcgtcggtg tagttggcgg ggttgaggga
     4801 ggggccgaag ccgaatatct tgaggccggg catctcctgg ttcttgaagg tcttgacgag
     4861 gccctcgtcg gcgtcggggt tgtagacggc agcgaatgcg ccccggtcgg gtgcggggag
     4921 ggcgaaggca cccagccagc cgttccaggt gtccatgcct gccaggtcct tagcattgtg
     4981 gttgggccag tcgatgatgc cgtgctcgga gggcaggctg ctatcgttgg tgctgtggac
     5041 caccagggtg cgggcgggca ctatgaagcg ggtgcgcggg gataccgagt tggtgccgcc
     5101 gggggcgagc atggcgttgg accagagctg gccaatggcc tgcctggtgg tgcggttctc
     5161 aaggcggctc gacgtggtgt aggcggcgtc ttcggggctg agggagatgg cgctcacgac
     5221 agtgaggccg gtgagcttgt cgttggcgga gagcgtgacg gtcgcgccgc cgtcgctgtt
     5281 gcgctggacc gaggcgtccc agggcaggta ctccatcatg ccgtgctcct gggtgggagc
     5341 ggcccattcc atgccgcctg aggccagcca ccagccgcgc tccccgaagc gggagggctt
     5401 gatgacgggg ttgcgataaa gctcattgtg cccggtcggc ttgtatatgg cttcgtagag
     5461 gcgaccgcct agctggggca gcatggtgag cttaatgtac ctgctttcca acacgagcag
     5521 gcggtaagtg cgcggggagg gcgggcctac cttgtcgaag tcgaggcggg ggtaggggga
     5581 gatagggtcg ttggcgggcg tgtagaaagc ctgctcgtaa gcgtaggtgg gaatggagac
     5641 ggtggtctcc cgcacctgca cgcgctgggc gaggaggtcg cgtccgagga ggccaagctg
     5701 cacttcgtac ggggtgccgg tgacttcggg gagcaactcg aagcggttgc gctcgaagta
     5761 ctgcacggtg aggccgcctt cctgtagcgg ctcggagatc gggtagccaa aaagggccag
     5821 gccgccgttg ccgcgccagt agcgcaggaa ggggccggag agggtatggc gggtctcagg
     5881 gaagtactgg gtatcggggc cggagggcgc gttaggctgg ggtacggtcg ggaactgccg
     5941 ccccgcggtg agtatgctgc cgagtaagcc aagctgcacc tcgtaggggg tgttgcggtt
     6001 ctgtgggtgg tactctatgc gggcgcgttc gaggtactgc accttgacgc cgccctcggt
     6061 ctcaggctcg gtgagggggt agccgaagat ggggacgccg cctttgccgt cgtagaaggg
     6121 aaggaattcg ggcgcgaggg tcgtgcctgt ctgggggtag aagcggcctt gagcctgggc
     6181 gtgagcggtg gcgagtggcc ttgtgggcgc ggctatgccg aggccgatag ccaggagcaa
     6241 ggctatggcg agggctttgt atttgcgtat gcgtggcgaa gtggagttca tttatccctg
     6301 ttggtgggca aagttaaagg cggactcaca tccgccccat gataacatcc cgatgtaaga
     6361 tgccggtcag gttgcacaat cgacgtgtct gtaatagtaa cgaatca
//
LOCUS       DASRWZ010000495         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_39850,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000495 DASRWZ010000000
VERSION     DASRWZ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39850"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VFH60_07725"
     CDS             <1..1198
                     /locus_tag="VFH60_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6313707.1"
                     /translation="YDDRGRPIPVSDRLVLAALEGDTVKDYVVSADGHRLRVLLTPIY
                     MRANGRIVAVLQVSTPVDIIDRRLSDLNWLLAGASGVLLLVAAGGSYLLTGRALNAVD
                     RVTRQVQQIEFSRDLTQRLPEPGSKDEIGHLVSTFNQLIERLQSALETQRRFVADSSH
                     ELRTPLTVIKSNIHLLRRSNDPAEKDELMEATEAEVSRLNRMVNNLLYMAQMEAGLDL
                     KPVLRPVELDSLLLEVFARARSIAALKNQKVVLSHEDIGTTMGDRDQLQHLLLNLVDN
                     AIKYTPDGGTISLGLWEEGDWTRLEVTDNGQGIPESELPNIFERFYRTQEARKSIRHG
                     SGLGLAIAHSIIEAHGGRIEVFSRVGEGTTFRIWLRKPQPQPGPEKGQSTGPLLTRAA
                     GARLGA"
     gene            1276..2070
                     /locus_tag="VFH60_07730"
     CDS             1276..2070
                     /locus_tag="VFH60_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6313708.1"
                     /translation="MSDYLNLRLEVDEDRGVALITINRPDKLNALNHLLLNELDAALD
                     EVTEHSRVWALVVTGAGTKAFVAGADINEIATLESQEDGVEFSRFGQHVFSRIEGLTI
                     PVIMAINGYALGGGLELAMCGDVRFASDTAQFGQPEVNLGVIPGYGGTQRLARLIGRD
                     RAKLLIFTGERIPAEEAYRLGLVDRVLPATDLVDEALELAATLANKAPMAIALTKMAI
                     NTGISLPLDEALELESQLFGQVVDTEDRREGANAFKEKRQPHWTGR"
BASE COUNT          424 a          729 c          653 g          320 t
ORIGIN      
        1 atatgacgac cggggccgcc ccatacccgt cagcgaccgg ctggtgctgg ccgccctcga
       61 aggcgacacg gtcaaggact acgtggtatc cgccgatggg cacaggctgc gcgtcctcct
      121 cacccccatc tacatgcggg caaacggccg catcgtcgcc gtgctccagg tctccacccc
      181 ggtggacatc atcgaccgga ggctgagcga cctcaactgg ctgctggccg gggcgtcggg
      241 cgtactgctc ctcgtggcgg ctggcggctc ctatctgctc accggccgcg ccctcaacgc
      301 ggtggaccgc gtgacccgcc aggtgcaaca gatcgaattc tcacgcgacc tgacgcagcg
      361 cctcccggag cctggcagca aggacgaaat cggccacctg gtgagcacgt tcaaccagct
      421 tatcgagcgg ctgcaatcgg cccttgagac ccagcgccgg ttcgtggccg actcgtcgca
      481 cgagctgcgc acgcccctca ccgtgatcaa gagcaacatc cacctgctcc gccgctccaa
      541 cgaccccgca gagaaagacg agctaatgga ggccaccgag gccgaggtgt cgcgcctcaa
      601 ccgcatggtg aacaacctgc tctacatggc ccagatggaa gcgggcctcg acctcaagcc
      661 ggtcctgagg ccggtggagc ttgatagcct cctgctcgaa gtgttcgccc gcgcccgctc
      721 tatcgccgcc ctcaagaacc agaaggtcgt gctctcccac gaggacatag gcaccacgat
      781 gggcgaccgc gaccagttgc agcacctcct gctcaacctc gtggataacg cgataaagta
      841 cacgcctgat ggcggtacga tcagcctggg gctttgggaa gaaggtgatt ggacgcggct
      901 ggaagtcacg gacaacggcc agggcatccc ggagtcggag ctgccgaaca tcttcgagcg
      961 tttctacagg acgcaagagg cccgcaagag catccggcac ggctccggcc tgggcctcgc
     1021 catagcccac tccatcatcg aggcccacgg cggtaggatc gaggtcttca gccgcgtcgg
     1081 ggagggcacc acgttccgca tctggcttcg caagccccag ccacaacccg gccccgaaaa
     1141 agggcagagc actggcccgt tgctcacccg cgccgccggt gcccgcctgg gtgcctagcc
     1201 tggctgtaga catgcccagc gtagccctgt gggcgtccat gtaaggcaac ccacaacaaa
     1261 caccgaggag caaacatgtc agattacctg aacctgcgcc tggaagtgga cgaagaccgg
     1321 ggcgtcgccc tcatcaccat caaccggccc gacaagctga acgccctcaa ccacctactg
     1381 ctcaacgagc tggacgcggc cctggacgag gttacagagc atagtcgcgt gtgggcgctg
     1441 gtcgtaaccg gcgctggcac caaggccttc gtagcgggtg ccgatatcaa cgagatcgcc
     1501 acgctggaaa gccaggagga cggcgtcgag ttctcccgct tcggccagca cgttttctcc
     1561 aggatcgagg ggctgaccat ccccgtgatc atggctataa acggctacgc gctcggcgga
     1621 gggctggaac tcgccatgtg cggcgacgtc cgcttcgcct ctgacaccgc gcagttcggc
     1681 cagccggagg taaacctcgg ggtcattccg ggctacggcg gcactcagcg cctggcacga
     1741 ctcatcgggc gagatcgcgc caagctgctg attttcaccg gcgagcgcat accggcggag
     1801 gaagcgtaca ggctcggcct cgtggaccgc gtgctgcccg ccaccgacct ggtggacgaa
     1861 gcgctcgaac tggccgctac cctggcaaac aaggccccaa tggccatcgc cctcaccaaa
     1921 atggcgatca acaccggcat ttcactgccc cttgatgagg ctctggagct tgagtcccaa
     1981 ctgttcgggc aggtcgtgga caccgaggac cgcagggagg gggccaacgc cttcaaggag
     2041 aagcggcagc cccactggac cgggcgctag gggcagttac gctcatgcgt aagagacgaa
     2101 accgccaaga cgccaaggac gccaag
//
LOCUS       DASRWZ010000496         1886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_314625, whole genome shotgun sequence.
ACCESSION   DASRWZ010000496 DASRWZ010000000
VERSION     DASRWZ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314625"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..>1886
                     /gene="topA"
                     /locus_tag="VFH60_07735"
     CDS             257..>1886
                     /gene="topA"
                     /locus_tag="VFH60_07735"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET6313709.1"
                     /translation="MATTTKKSGTTASSRKSTKTTKPAAKSRSTTSRASSASATGSRS
                     KSAGSSDGPIIRGNTNLVIVESPAKARTIKKYLGRNFTVEASMGHVRDLPKSTLGVDV
                     ERNFNPQYVVPRDKSKTVKDLRQRVQAARAVYLATDPDREGEAIAWHVREVTGAGNNN
                     QPVFRVEFHEITPHAIQEAVANPRSIDMHLVDAQQARRVLDRLVGYRLSPLLWKKVRR
                     GLSAGRVQSVAVRLIVDREREIEAFVPVEYWSIEADLSKRPAGKKKDQFHAALALVNG
                     KKADLKNEQDAAGIVRALHGSDYVVESVKRREAQRRPSPPFTTSTLQQEASRKLNFAA
                     RKTMLIAQQLYEGVDIGPEGSTGLITYMRTDSTNVATVAQQEAREVIRERYGAAFVPA
                     SPPAYARKNPRAQEAHEAVRPTSAARDPESIKKFLSADQYKLYKLTWQRFIASQMANA
                     ILDQTTVDVGAGKPGTSGTRPYTFRATGSVVKFEGYTAVYREGHDAGDAEDDMDKAAL
                     PPLAEGENLDLMKLWPEQHFTQPPPRFTEATLVKS"
BASE COUNT          392 a          681 c          548 g          265 t
ORIGIN      
        1 gcctacccac ctgatccttc gctacgctca ggatgacaaa ggggaatatg ctcgccgcgt
       61 ttgacacgta ccattcacgg acccagtggc gtccgtcgtc catcgtcact actgccgcct
      121 tgacaccgcc ccatactgag cgtaagataa gaagcagccg tacgtgatgc acgctcgaaa
      181 gagcgtcttt ttgcttgaaa atgagttttt cagcctgata ttgagtttac atacagcgca
      241 aaggtccacc taactaatgg caactacgac taaaaagagc ggcactaccg cttcgtctcg
      301 caaatcaacc aagaccacca aaccggccgc caagagccgt tccaccacct cccgcgcgtc
      361 ctccgcctcc gccaccggct ccaggagcaa gagcgcgggc agcagcgacg gacccatcat
      421 ccggggcaac accaacctgg tcatcgtgga atcgcccgcc aaggccagga ccatcaagaa
      481 atacctgggg cgcaacttca cggttgaggc cagcatgggc cacgtccgcg acctccccaa
      541 gtccacgctc ggagtggacg tggagcgcaa cttcaacccg cagtacgtcg tgccccgcga
      601 caagtcgaag acggtgaagg acctgcgcca gcgcgtgcag gccgccaggg ccgtctacct
      661 ggcgaccgac cccgaccgcg agggcgaggc catcgcctgg cacgtgcgcg aagtcaccgg
      721 cgcgggcaac aacaaccagc ctgtgttccg cgtggagttc cacgagatca ccccacacgc
      781 catccaggag gccgtagcca atccacgcag catagacatg cacctcgtgg acgcccagca
      841 ggcccgccgc gtgctcgacc gcctggtggg ctaccgcctg tcgcccctcc tctggaagaa
      901 ggtgcgccgg gggctgtcgg cgggccgcgt gcagagcgtg gccgtgcgcc tcatcgtgga
      961 ccgcgagcgc gagatagagg ccttcgtgcc cgtcgagtac tggagcatcg aggccgacct
     1021 cagcaagcgc cccgccggca agaagaagga ccagttccac gccgccctcg ccctcgtgaa
     1081 cggcaagaag gccgacctca agaacgagca ggacgccgcg ggaatcgtgc gcgccctcca
     1141 cggctctgat tacgtggtcg agagcgtcaa gaggcgggag gcccagcgcc gcccgtcgcc
     1201 ccccttcacc acctccacgc tacagcagga ggcctcgcgc aagctcaact tcgccgcccg
     1261 caagacgatg ctcatcgccc agcagcttta cgagggcgtc gatatcggcc ccgaaggctc
     1321 taccggcctc atcacctaca tgcgcaccga ctcgaccaac gtagccacgg tggcccagca
     1381 ggaggcccgc gaggtcatca gggagcggta cggcgcggct ttcgtgcccg cctcgccccc
     1441 cgcctacgcc cgcaagaacc cccgcgccca ggaagcccac gaagcggtcc gccccaccag
     1501 cgccgcccgc gaccccgagt cgataaagaa gttcctctcc gccgaccagt acaagctgta
     1561 caagctcacc tggcagcgct tcatcgcctc ccagatggcg aacgctatcc tcgaccagac
     1621 gacggttgac gtgggcgcgg gcaagccggg tacgagcggc accaggccct ataccttccg
     1681 ggctacaggc tcggtggtca agttcgaggg ttacaccgct gtgtaccgag aggggcacga
     1741 tgcaggcgac gccgaggacg acatggacaa ggcagccctg ccgccgctgg ccgagggcga
     1801 aaacctcgac ctgatgaagc tttggcccga gcagcacttc acccagccgc cgccccgctt
     1861 caccgaggcg acgttggtca aatcgc
//
LOCUS       DASRWZ010000497         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_245576, whole genome shotgun sequence.
ACCESSION   DASRWZ010000497 DASRWZ010000000
VERSION     DASRWZ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245576"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1867)
                     /locus_tag="VFH60_07740"
     CDS             complement(74..1867)
                     /locus_tag="VFH60_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET6313710.1"
                     /translation="MNAQHTEITGPEALDGLEVGAPPEASGTTNVEFPWQAHYEAQVP
                     PEIEVPGLTLHEFFERTARDYPNNIAAIFFSRRLTYAQLDEQANRFASGLQALGVQPG
                     DRVAILLPNCPQFLIALFGALKAGAVAIPLNPVYVSRELRQVFNDADVRTVVALNVLL
                     PRLEEIVPETSLQRIVTTSMQDYLSPLMSLMLTVKERREGTILPTQGEGLYLYTDLIR
                     NSPIEYARSQAGPDDTALFLYTGGTTGTPKGAMLSHRNVVANALQMSAWVWDARPERK
                     EVFLGVIPFFHSYGMTVVMNLAISVAASIVLLPRFNMKDVLRAIARFRPTIFPAVPTM
                     YNAIAHHPLSARFDLRSIRVCISGAAPLPVEVASAFESVTGARLVEGYGLTEASPVTH
                     CNPIHGERRAGSIGLPLPSTEARIVDPETREPLATGEVGELAVRGPQVMQGYWGHEEE
                     SAAVIGEGWLYTGDMARMDEEGYFYVVDRKKDLILVGGYSVYPREVEDALYESEKVQE
                     VVVVGVPDVKRGEGEIVKAYVVLNGDVEATERELVKFAAERLAPHKVPGKIEFRESLP
                     KSGVGKYLRRELVAEVLARASSEEAEPPSMP"
BASE COUNT          337 a          704 c          632 g          393 t
ORIGIN      
        1 ctagctcaaa tgcttccatt cattaatcct cttggcgtta tacttggctt ccttggcgtc
       61 ccttcgactt cgctcagggc atgcttggcg gttccgcctc ctcagaagag gcccgcgcca
      121 acacctccgc cactaactca cgccgcaggt acttgcctac gccgctcttg ggaaggcttt
      181 cgcggaactc tatcttgcct ggtactttgt gcggggccag acgctcggcg gcgaacttga
      241 ccaactcgcg ctcggtggcc tccacgtcgc cgttgagcac cacgtacgct ttgacgatct
      301 ccccctcacc ccgctttaca tcgggcaccc ccaccaccac tacttcctgc accttctcgc
      361 tctcgtacag cgcgtcctcc acctcgcggg ggtagacgct gtagccgccc accaggatga
      421 ggtccttctt gcggtccacg acgtagaagt agccctcctc gtccatgcgc gccatgtcgc
      481 ccgtgtagag ccagccctcc ccgataacgg ctgcactctc ctcctcgtgc ccccagtacc
      541 cctgcatcac ctgcggcccg cgcaccgcaa gctctcccac ctcccccgta gccagcggct
      601 cccgcgtctc cgggtccacg atgcgggcct ccgtgctggg gagtggcagg ccaatgctgc
      661 ctgccctccg ctccccgtgt atggggttgc agtgggtgac gggcgaggcc tcggtcaggc
      721 cataaccctc caccagccgc gctcccgtga cgctctcgaa cgcgctggcc acctccaccg
      781 gcaggggggc cgctccactg atgcacaccc ggatggagcg caggtcgaag cgggccgaca
      841 gcgggtggtg cgcgatggcg ttgtacatcg tgggcaccgc cgggaagatg gtgggccgga
      901 agcgcgctat agcccgcagc acgtctttca tgttgaagcg cggcagcaac actatcgagg
      961 ccgccaccga aatcgccagg ttcatcacca cggtcatgcc gtagctgtgg aagaagggta
     1021 tcacccccag gaacacttcc ttgcgctccg gccgcgcgtc ccacacccac gcgctcatct
     1081 gcaaggcgtt ggctacgacg ttcctgtggc tcaacatcgc gcccttgggg gtgcccgtgg
     1141 taccgcccgt gtagaggaag agcgcggtgt cgtcgggacc ggcctgcgag cgcgcgtact
     1201 cgattgggct gttgcgaatc aggtcggtgt agaggtagag gccctcgccc tgcgttggca
     1261 ggatggtccc ctcccgccgc tccttcaccg tgagcatcag cgacatgagg ggcgataggt
     1321 agtcctgcat agaggtcgtc acgatgcgct gcaaggaagt ctcaggcacg atctcttcca
     1381 gccggggcag cagcacgttg agcgccacca cggtgcgcac gtccgcgtcg ttgaacacct
     1441 ggcgcaactc tcgcgacacg tacaccgggt tgaggggtat agccacggca cccgccttga
     1501 gcgcgccgaa cagggcgatc aagaactgcg ggcaattggg cagcagtata gctacccggt
     1561 cgcccggctg cacgccaagc gcctgcaacc ccgacgcgaa gcggttggcc tgctcgtcaa
     1621 gctgggcgta ggtcaggcgg cggctgaaga agatcgcggc gatgttgtta gggtagtcgc
     1681 gggcggtgcg ctcgaagaat tcgtgcaggg taagccccgg cacctctatt tcggggggca
     1741 cctgcgcctc atagtgggcc tgccagggga actcgacgtt agttgtgccg gaggcttcgg
     1801 gcggtgcccc tacctccagg ccgtccaatg cctcaggccc cgttatttcg gtgtgctgtg
     1861 cgttcattcg taaatagctc ctacctctcc taccgggatt gtatgcacgg gcaagatggt
     1921 tgtcaaggca aagcgggtgt gttatcgtga cggctgaacg tacaacctgg ggggagcatg
     1981 gacccggacc ttagcatcgt catacttagc tggaacgtgc gcgacctgct ggcgggctgc
     2041 ctgcgctccc tgccccgggc cgccgc
//
LOCUS       DASRWZ010000498         5486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_931517, whole genome shotgun sequence.
ACCESSION   DASRWZ010000498 DASRWZ010000000
VERSION     DASRWZ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931517"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFH60_07745"
     CDS             <1..358
                     /locus_tag="VFH60_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313711.1"
                     /translation="DPNDPHALSLAELRRSVEAVNEVWFVSAHLPITWDVRIFGRMLA
                     YAKRVTRLLLRWYINPIVEQQNRFNSATARAVTVMNAYQERMTREWMLLEERVAKLEE
                     EVRGREAGSGGQGDGL"
     gene            358..1479
                     /locus_tag="VFH60_07750"
     CDS             358..1479
                     /locus_tag="VFH60_07750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313712.1"
                     /translation="MGHEPHAARFAPQEDTAGPSPAASPQGSLRIAICTTQVPFVYGG
                     NEVLVEGLRDALVERGHRVSIISIPYRWHPRKHIVTGALAWRLIDITESNGEPVDLAI
                     CTKWPSYVVKHPRKVVWLVHQFRQAYDWFGTPLSDFTSLPEDRRVRRLILKMDHTALS
                     ESRGVFTISENVANRLRRFNGLEGTPLYPPLRSGLSLEPGPYGDYVFSLNRLDPAKRI
                     VLLLEALAQAPEVRAVIAGTGPEADNLKRQATRLGVANRVEFAGFVSDEEASRLYRSA
                     GAVYYAPVDEDYGYGTIEAFQAARPVITTSDAGGVLEFVEHGVTGLVSPPEPAAIARS
                     LQRVVSNREEAEMLGRAGREKVRDITWDRVVDALLGAAG"
     gene            1538..2626
                     /locus_tag="VFH60_07755"
     CDS             1538..2626
                     /locus_tag="VFH60_07755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002784704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313713.1"
                     /translation="MARHSSSSTIPTMHILITTSQVPFIRGGAELHAEGLRDALRAEG
                     HDAEIVAIPFKWYPAERILAHMAACRLLDLSEIGRMAIDMVIGLKFPAYLVPHPNKVL
                     WILHQHRQAYELWEHPMGDLHLEPNGAYVREAIRQADRTYIREARAVYANSKTVAGRL
                     KEYSGLDATPLYHPPHQADKFYSAEAEDYLLFPSRIGTMKRQELVVAALALTTQPVRV
                     SFMGAADREEYARKLRDMSRELGLEGRVQWLGPVSDEEKFRLYAHSLGVLFPPADEDY
                     GYITLEAMLSSRPVITCTDSGGPLEFVRDGETGYVTDPTPEGIAAAMDQLWEDRTGAK
                     SLGKAGRALYDSLGISWTTVVKHLLSGP"
     gene            complement(2693..3199)
                     /locus_tag="VFH60_07760"
     CDS             complement(2693..3199)
                     /locus_tag="VFH60_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313714.1"
                     /translation="MTNMVAFSTLERTQTLSTGLVVALSRDLASIQGPDGRLVAADSL
                     GYGLVQQSTGEYVLVRWIGAEFECWVEQSDLRSIGPDARLVTVEKYDNKGTRKQVRHK
                     LGYNAGLRNNWTVELRPQGIVRVLRVDGHKWTFTHNKLFRRIDVGWPQPPDDEDAEAL
                     TVAELAIR"
     gene            3680..>5486
                     /locus_tag="VFH60_07765"
     CDS             3680..>5486
                     /locus_tag="VFH60_07765"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6313715.1"
                     /translation="MLDPQGYTFTLYSLPTLLTMAAVFSLGVFVLVRERITLVSVSFF
                     IVTLAVSNWLFCFSWMYAANNPATAMWWSRAAYLGVPFIAPAAYQFTAVVLGLHRRLR
                     RVILAAWLVSALFAVAAVATDNLITGVERYWWGYYTRFEWLGLAFVAFFAALLVGSMA
                     HYWTVYKQLQRGRARRRIRWLMLAFGVGYLGIVDFVAVYGLPLYPFGYIAILAFVALA
                     TRAIWTYHLIDITPAFAANEIIETMNDALLVFDREGVLRVANRAACEMFGYEKEELLG
                     RPPGSTLGGVNFTGQLESLVQGGRVRDYELTYSPREGGTRFLSIAASVMRSADGEPVA
                     IVCIAHDITERRAAENEIRSLNESLEQRVAQRTEELQIANRELENEIAERRRAEEERT
                     LLLDREQAARAAAEQAAHSREVMLSVVSHDLRNPLTAVKATVSMLRQALRQTETGGPS
                     AERMQVGLARIDTAANKMDMMINELLDFAQLQAGQPLELYRRNFDLVEMCRRVVAEYQ
                     QSARRHQLVLETALPRLVGQWDPMRIERVLDNLISNAIKYSPDGGPISIEMGSEGPAE
                     GGDGVGHDWVTLVVRDQGVGIPEADVPYIFDWFRRA"
BASE COUNT         1034 a         1673 c         1789 g          990 t
ORIGIN      
        1 cgaccccaac gacccccacg cgctgagcct ggccgaactg cgccgcagcg tggaggcggt
       61 aaacgaggtc tggttcgtgt cggcccacct ccctatcacg tgggacgtgc ggatattcgg
      121 caggatgctg gcctacgcca aacgcgtgac gcgcctgctg ctgcgctggt acatcaaccc
      181 cattgtggag cagcaaaacc gcttcaactc ggccaccgcc cgcgccgtca ccgtgatgaa
      241 cgcctaccag gagcgcatga cccgcgagtg gatgttgcta gaggagcgcg tggcgaaact
      301 ggaggaggag gtaaggggcc gggaagcggg cagcggaggc cagggagacg gcctttagtg
      361 ggccacgaac cacacgccgc aaggtttgct ccccaggagg atacggcagg cccttcaccg
      421 gcagcttcgc ctcaaggctc tctgcgcatc gctatttgca ccacccaggt gcctttcgtg
      481 tacggcggca acgaagtgct ggtggaaggg ctgcgggatg cgctggtgga gcgggggcac
      541 agggtctcga tcatctccat accctacagg tggcaccctc gcaagcacat cgtcaccggc
      601 gcgctggcct ggcggctcat tgacataacc gaatcgaacg gggagccggt ggacctggcg
      661 atatgcacca agtggccctc ttacgtggtg aagcacccgc gcaaggtggt gtggctggtc
      721 caccagttcc ggcaggccta cgactggttc ggcacgccgc tatccgactt cacgagcctg
      781 cctgaggaca ggcgggtgcg ccgcctcatc ctgaagatgg accatacggc gctgagcgag
      841 agccgagggg tcttcaccat atcggagaac gtagccaacc gcctgcgccg cttcaacggg
      901 cttgagggca cgcctctcta cccgcccctg cgctccggcc tctccctgga acccggcccg
      961 tacggcgact atgtcttttc gctcaacagg ctggaccctg caaagcgtat cgtcctgctg
     1021 ttggaggcgc tggcccaggc ccctgaggtg cgagccgtaa tcgcgggcac ggggccggaa
     1081 gccgacaacc tcaagcggca ggctacgaga ctcggtgtag ccaaccgggt ggagttcgcg
     1141 ggcttcgtct cggacgagga ggcttcgcgg ctgtacagga gcgccggggc cgtctactac
     1201 gcgcccgttg acgaggacta cggctacggc acgatagagg cgttccaggc ggcgcgcccc
     1261 gtcatcacta cgagcgatgc ggggggcgtg ctcgaattcg tggagcacgg cgtgacgggc
     1321 ctggtaagcc cacctgagcc tgccgccata gcccgctctc tgcaacgcgt ggtctccaac
     1381 cgggaggagg ctgagatgct gggcagggca gggcgggaga aagtgcggga tattacctgg
     1441 gatagggtag tggatgcgct gcttggggct gccggttaga cgctagacgg tgggaacgcc
     1501 ctgccatgtt gccctgtggg gacatggcag ggcgaacgtg gcaaggcatt cctcatcgtc
     1561 caccattcca actatgcata tcctgattac aaccagccag gtgccattca tccgcggagg
     1621 ggccgagctt cacgcggagg ggctgcgcga tgccttgcgg gccgaggggc acgatgccga
     1681 gatagtcgcc atccccttca agtggtaccc cgccgagcgc atcctggccc acatggcggc
     1741 gtgccgcctg ctcgacctga gcgagatagg tcgtatggcg attgacatgg tgatagggct
     1801 gaagttcccc gcttacctgg tgccccaccc caacaaggtg ctctggatat tgcaccagca
     1861 ccggcaggct tacgagctgt gggagcaccc gatgggcgac ctgcacctgg agccgaacgg
     1921 ggcctacgtg cgcgaggcga tccgccaggc cgaccgcacc tacatcagag aggccagggc
     1981 ggtgtacgcc aactcgaaga ccgtggccgg gcggctcaag gagtacagcg gcctcgacgc
     2041 gaccccgctg taccacccac cccaccaggc ggacaagttc tacagcgccg aggccgagga
     2101 ttacctgctc ttccccagtc gcatcggcac gatgaagcgg caggaactgg tggtggcggc
     2161 gcttgctttg acgacgcagc cggtaagggt gagcttcatg ggcgcggcgg accgagagga
     2221 atacgcccgg aagttgcggg acatgtcgcg ggagcttggt ttggaaggcc gggtgcagtg
     2281 gctcggcccc gtgtccgacg aggagaagtt caggctgtac gcccactccc tcggcgtgct
     2341 cttcccgccc gcggacgagg actacggcta catcaccctc gaagcgatgc tgtcgtccag
     2401 gccggtgata acctgcaccg actccggcgg ccccctagaa ttcgtccgcg acggcgagac
     2461 cggctacgta accgacccca cccccgaagg cattgccgcc gcaatggacc aactgtggga
     2521 ggaccgcacg ggcgcaaaat cgctaggcaa agcgggcagg gccttgtacg acagcctggg
     2581 catctcctgg actacggtag taaagcatct cctatcagga ccatagcccc aatcaggaac
     2641 aaagccccac caagcaatta agaattaagc attaaaaatt aagaattacc ccttaccgta
     2701 ttgccagctc ggccacggtg agggcctcgg cgtcttcgtc gtcggggggt tggggccacc
     2761 ctacgtcgat gcggcggaag agcttgttgt gggtgaaggt ccacttgtgg ccgtcgacgc
     2821 gcaacacgcg gactatgccc tggggccgca attctacggt ccaattgttg cgcaagccgg
     2881 cgttgtagcc tagcttgtgc cgcacttgct tgcgcgtgcc cttgttgtcg tacttctcaa
     2941 ccgtgaccag gcgagcgtcg gggcctatcg agcgcaggtc cgattgctcc acccagcatt
     3001 cgaactccgc cccaatccag cgcaccagca cgtactcccc ggtggactgt tgcaccaggc
     3061 cgtagcccag gctgtcggcg gcgacgagcc tgccgtccgg cccctgtatg gacgccaggt
     3121 cgcgcgacag ggccaccacg aggccggtag agagagtttg agtcctctcc aacgtgctga
     3181 atgcaaccat gttggtcatg tggcacctcc tcgtgcccga aacgccatta cggggtgccc
     3241 cggcactatc ccctcaagct aattatagct agagcaacct tgtttgtaca ctcataacct
     3301 atatgcttgt agcgatatgt aggttccata ggggcatcct gtaccctaat gttgtaatat
     3361 agaaggcact ccccgttaag tcgtagtgag gctaccttca agtaagggcc ggaaagaaac
     3421 ggattcgtat acggtctcaa cgatacaact ggatgacagc agccaaagtc ccgcccctgt
     3481 cattctgagc gtagcgaagt atctcgtcct ctaccccgat gttgctcttt tcttgcggga
     3541 ttctggtggc gttgcacaag gggaggtctg ggtgctgagg gacgagattt cgaacgcagt
     3601 gagaaatgac aggggcgggg ctttggctgc cagtatttga tgctcctaag ttgcgatagc
     3661 ttctagtatg gttacacagg tgctggaccc tcaaggctat accttcacct tgtactcgct
     3721 gcccaccttg cttacgatgg cggcggtctt ttcgcttggg gtgttcgtgc tcgtccgcga
     3781 gcgtatcacg ttggtcagcg tctccttctt catcgtgacc ctggcggtga gcaactggct
     3841 cttctgcttc tcctggatgt acgcggccaa caacccggcg accgcgatgt ggtggtcacg
     3901 agccgcatac ctgggggtgc ccttcatagc gcccgcagcc taccagttca cggcggtggt
     3961 gctgggcctc caccggcgct tgaggcgggt gatactcgcg gcctggcttg tttcggccct
     4021 gttcgccgtc gcggcggtag ccaccgataa cctcatcacg ggcgtggagc ggtactggtg
     4081 gggctactac acccggttcg agtggttagg gctggccttt gtggcgttct tcgcggcgct
     4141 gctggtgggc agcatggctc actactggac ggtctacaag cagttgcaac ggggcagggc
     4201 caggcggcgc atccggtggc tcatgctggc gttcggggtt ggctacctgg gtatcgtgga
     4261 cttcgtggcc gtgtacggcc tgcccctcta tcccttcggc tacatcgcga tcctggcgtt
     4321 cgtggcgctg gcgactcgcg ccatctggac ctaccatctc atcgatatta cccccgcctt
     4381 cgccgctaat gaaattatcg aaacgatgaa cgacgcgctg ctcgtgttcg accgggaggg
     4441 tgtgttgcgg gtcgcgaaca gggccgcctg cgagatgttc ggttacgaga aggaggagct
     4501 gcttggcagg ccgcccggca gcacgctagg cggggtcaac ttcaccgggc aactggagtc
     4561 gctagtccag ggcggaaggg tacgcgacta cgagctgacg tactctccca gggaaggcgg
     4621 gacgcgcttc ctcagcattg ccgcctctgt gatgcgcagc gcagacggcg agcctgtcgc
     4681 catagtttgc atagcgcacg acatcacgga gaggcgggcc gctgaaaacg agatacgcag
     4741 cctcaacgag agcctggagc agcgggtggc gcaacgcacc gaagaactgc aaatagccaa
     4801 ccgcgagctt gagaatgaaa tcgccgagcg gaggcgcgca gaagaggaac gcaccctgct
     4861 actcgaccgc gaacaggccg cgcgggcggc agcggaacaa gccgcgcact cgcgtgaggt
     4921 gatgctgtcg gtggtatctc acgacctgcg aaatcccctc accgccgtga aggccacggt
     4981 tagcatgttg aggcaggcgc tcaggcagac cgagacggga gggccgagcg ccgagcgcat
     5041 gcaggtaggg ctggcgcgca tagacacggc ggctaacaag atggacatga tgattaacga
     5101 gctactcgat ttcgcgcagt tgcaggcggg gcaacccctg gagctatacc ggcgcaactt
     5161 cgacctcgtg gagatgtgca ggcgcgtggt tgccgagtac cagcaaagcg ccaggcggca
     5221 ccaactggta ctcgagacgg ccctgcctcg gttggtaggc cagtgggacc ccatgcgcat
     5281 cgagcgggtg ctggacaacc tgatctccaa cgccatcaag tacagccccg acggcggccc
     5341 gataagcatc gagatgggga gcgagggtcc ggcagagggt ggcgatggcg tgggccacga
     5401 ttgggtgacc ctcgtggtga gggaccaggg ggtaggcatc ccggaggcag acgtgcccta
     5461 catcttcgac tggttcaggc gcgccc
//
LOCUS       DASRWZ010000499         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_109290, whole genome shotgun sequence.
ACCESSION   DASRWZ010000499 DASRWZ010000000
VERSION     DASRWZ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109290"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFH60_07770"
     CDS             <1..641
                     /locus_tag="VFH60_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6313716.1"
                     /translation="KRPRLISNRGLVVRLLLPGSLVGMGAGMTIPFMNVYIERKFNVS
                     FENLGQIFAWTAIATAAALMVQPVLAGKMGKVKSVVLVQGLSLPFLLVLGYAQFFPLV
                     VAALFVRGALMNMGNPVFSAYSMERVPDRERATFTSLTSSTWALGWASGSWFSGTLRD
                     ALGFFEGFNVLWAIMAVLYASSMLLMWFWFAGHEARQLQEQALQQESHASAA"
     gene            complement(843..>1946)
                     /locus_tag="VFH60_07775"
     CDS             complement(843..>1946)
                     /locus_tag="VFH60_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313717.1"
                     /translation="PPSGETRSGRRRTAALDPARTVAVREVSLQVRRGEVLSLLGPSG
                     CRKTTVLRLLAGLERPDGGEVRVNGQLVSGRGSWVPPEKRRVGLVFQDYALFPHMTVA
                     RNIAFPLSRWPATKREARVTELLEMIGMPGMGNRYPHQLSGGQQQRVALARALAPEPD
                     LVLMDEPFSSLDAEGRATTRDQVRSILKSLGTTVIFVTHDQEEALLLGDRVAVMNAGQ
                     VEQVGTPEAIFQSPATRFVAEFLGLSCFLPGVVTPVGLQTEIGLNPQAAGAPCGTAVE
                     VLVRPDDLLVRADPLGDARVVRCMYRGMDYLYDVALPSGTTVRCIGTHTVKLSPGTPV
                     RLELSAGHALTCVRREDGHCRLCEPAPETASLS"
BASE COUNT          327 a          643 c          625 g          351 t
ORIGIN      
        1 gcaagcgccc ccggctcatt tccaatcggg ggctggtggt gcgcctgctg cttcccggct
       61 cgctggtggg catgggcgcg ggcatgacga tcccgttcat gaacgtgtac atcgagcgca
      121 agttcaacgt gagcttcgag aacctggggc agattttcgc ctggaccgcc atagccacgg
      181 ccgccgcgct catggtgcag ccggtgctcg ccgggaagat gggcaaggtc aagtcggtgg
      241 tgctggtgca ggggctttcg ctgcctttcc tgctggtgct cggctacgcg cagttctttc
      301 cgctcgtggt ggcggccctc ttcgtgaggg gcgcgctgat gaacatgggc aaccccgttt
      361 tctcggccta cagcatggag cgcgtgcccg accgggagcg cgccaccttc accagcctca
      421 cttccagcac gtgggcgctc ggctgggcct caggctcctg gttcagcggc accctgcgcg
      481 acgcgttagg cttcttcgag ggcttcaacg tgctatgggc catcatggcc gtgctctatg
      541 cctcctccat gctgctgatg tggttctggt tcgccggaca cgaggcccgc cagctacaag
      601 aacaagccct gcaacaagag tctcacgcct ccgcagcttg agaatagacg aaaacgccaa
      661 gacgccaggg accacgcgaa gagtacgaaa acgcaaagat taggattgta tggtgggtac
      721 aaagaaggag cagggtacat gatgacgtac cctgctcctt cttgtattct ctcaaccttt
      781 attaacgtga ttaacgtggc gtggtgcttg gcgtcttggc gtcttggcgt tttcgtctat
      841 tcctatgata acgatgcagt ctccggcgca ggctcgcaca ggcggcagtg gccgtcctcg
      901 cggcggacgc aggtgagcgc gtggcccgct gaaagctcca ggcgcacagg cgtgccgggg
      961 gatagcttga ccgtatgggt gccgatgcac cgtaccgtgg tgccggaagg cagggccaca
     1021 tcgtacaggt agtccatgcc gcgatacatg cagcgcacca cgcgggcatc acccaggggg
     1081 tctgcgcgta ccagcaggtc atcgggccgc accagcacct ccacggcggt accgcacgga
     1141 gcgcctgcgg cctgcggatt cagacctatc tccgtctgca agcccaccgg cgtcaccacc
     1201 cccggcagga agcaggacaa ccccaggaac tcggctacga agcgcgtagc aggactctgg
     1261 aatatagctt cgggggtgcc gacctgctct acctggcccg cgttcatcac ggctacgcgg
     1321 tcgcccagca aaagcgcctc ctcctggtcg tgggtcacga aaattaccgt ggtgccgagg
     1381 ctcttgagta tgctgcgcac ctggtcgcgg gtagtcgcgc ggccctcggc atctaggctg
     1441 ctgaaaggct cgtccataag caccaggtcg ggttcggggg caagggcgcg ggccagggcc
     1501 actcgctgct gctgccctcc cgacaactgg tgcgggtagc gattgcccat gccgggcatc
     1561 ccgatcatct ccagcaattc ggtcacgcgc gcctcgcgct tcgtggcggg ccaccgggac
     1621 agtgggaacg cgatgttcct ggcaacggtc atgtggggga agagcgcgta gtcctggaag
     1681 accaggccga cgcgccgctt ctcgggaggc acccacgagc ctcgcccgct gacgagctgg
     1741 ccgttcacgc gcacctcccc gccgtccggt ctctccaggc ccgcgagcag ccggagcacg
     1801 gtggtctttc tgcaccccga cggccccagc aacgacaata cctcgccgcg ccgcacctgc
     1861 aaggatacct cccgcaccgc gacggtccgt gccgggtcca gcgcggcggt ccgcctccgg
     1921 cccgaccgag tctccccgct tggcgg
//
LOCUS       DASRWZ010000500         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_384157, whole genome shotgun sequence.
ACCESSION   DASRWZ010000500 DASRWZ010000000
VERSION     DASRWZ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384157"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1227
                     /locus_tag="VFH60_07780"
     CDS             97..1227
                     /locus_tag="VFH60_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6313718.1"
                     /translation="MTDALTTQLDPERQRTAKEYAGLKRRLFFVDLALAILLVLALLF
                     LGWSVAVRDWAESISDSPWLVIALYSVALGAGYTLLSLPLGFYAGHVLPHRYGLSTQS
                     LGGWVSDTVKELAISAVFGLGAMEVLYWLLRSLPEWWWVVMAGMAWLFMVAMTQLAPV
                     LLMPLFYKFRPLEDAELVARLTRLAESAGAKVRGVYVMDMSSRTTAANAMLTGLGATR
                     RIILGDTLLSNYTYDEIETVLAHELAHHVHNDLPKGLAAEAAILLASMGAAALLLNWG
                     VPAFGFRGIDDVAALPLFIVALAVVGIVAMPVGNFLTRQMERAADRYAIRTTGKPHAF
                     RSVMLKLAGQNLAEAEPPAWVRVLFQSHPTIAERVASAEENS"
     gene            1444..2556
                     /locus_tag="VFH60_07785"
     CDS             1444..2556
                     /locus_tag="VFH60_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6313719.1"
                     /translation="MTTDAAPSGRINPLANVLDWDVSAAYELLLSLRAIFLPKDYGIP
                     APWAAGVRKRLSPAGQHDLKGFFNPSFAVIAYAPIHLVLEMEPPKTVERFFDYVEAIP
                     GPDFMRRIYAPTKFQDIPADVKDRALAGKKLSDADVEEYRRGIARAGIGSTPSVADVR
                     RLFEDMANPEATKQRWLNVMREYQAVFFADEEKRLLPILERMLDDAKALAQKTTVPDL
                     IERLSNGFTLSPDIELDRLVLVPSVWLHPYVIRAEISESELLVAWGAHPPGHRVVPGE
                     SVPEDALLVLRALADPTRLRLLRLIAAEPRSLQSLAQEVKLSLPTVSHHIRELRGAGL
                     IRLEVGGRGRESKYTVRWPSAERAFDDLKQFVLTET"
     gene            2718..>3910
                     /locus_tag="VFH60_07790"
     CDS             2718..>3910
                     /locus_tag="VFH60_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313720.1"
                     /translation="MEDEILRFAQNDNGGEGHAVPTQPHSRTLLSKPPRPSRSTLQDH
                     RPALLSLILLAALYLAALVSMPPDGLTQHDAGAKYLQVRNLRLAPSGLDWSINYPARP
                     LDPELQFVPFRDRQHYIDGESIYLQWPIFLGLLTRIPWKVLGFWGLYVVPLLAGLGTL
                     WAAYILALKAGVKPRLAWAAIPLLGLATPLSIYSLLFFEHTLAALLVTLSLLSGVVAL
                     SSERPASRALWLNAALLAVAIYFRSELYVLALVTGLAYAYMAWRDGAWRGALVRGVGA
                     FAVSLVPLWAFYAITEGTILPLHAIWYFQGSDASSSGGLELPAVRYLASAGWRVVPDF
                     LFGPQSFPSSPRFPLWAELAGLLGLVLCALPGMARLLRPRGTIGRWRMPVVTVGLLLV
                     AIPSL"
BASE COUNT          626 a         1350 c         1172 g          762 t
ORIGIN      
        1 ctacttccca gcccgtggct ttagccacag ggctatctct gggctcccca gcattcgcaa
       61 acttctcacc actgaccact gaccactatt caccaaatga ctgacgccct caccacacag
      121 cttgatcccg aacgccagcg cacggccaaa gagtacgcgg gcctcaagag gcggctcttt
      181 ttcgttgacc tcgcgctggc tatcctgctg gtgctggcgc tgctcttcct cggctggtcg
      241 gtggccgtga gggactgggc cgaaagcatc tcagacagcc catggttggt gatagccctc
      301 tactcggttg cgctgggcgc gggctacacc ctcctatcgc tgcccctcgg cttctacgca
      361 gggcacgtgc tgccccaccg ctacggcctg tccacgcaga gcctcggcgg ttgggtcagc
      421 gacaccgtga aggagcttgc gatttcggcg gtgttcgggc tgggcgcgat ggaggtgctc
      481 tactggctgt tgcgcagcct gcctgagtgg tggtgggtcg tcatggccgg catggcgtgg
      541 ctcttcatgg tggcgatgac ccagctagcc cccgtgctgc tgatgcccct cttctacaag
      601 ttccgcccgc tggaagacgc cgaattagtg gcgcgcttga cccggctggc ggaaagcgcg
      661 ggcgcgaagg tgcggggcgt gtacgtcatg gacatgagca gccgcaccac cgccgccaac
      721 gccatgctca ccggcctggg tgcgacccgc cgcatcatcc tgggcgacac cctcctgagc
      781 aactacacct acgacgagat agaaacggtg ctcgcccacg agcttgccca ccacgtccac
      841 aacgacctgc ccaagggcct cgccgccgaa gctgccatcc tgctggcgag catgggggcc
      901 gccgccctgc tgctaaactg gggcgtgccc gcgttcggct tccggggcat agacgatgta
      961 gccgccctcc ccctcttcat agtcgccctg gccgtcgtcg gcatagtagc catgccggtg
     1021 ggcaacttcc tcacccgcca gatggagcgc gccgccgacc gctacgccat ccgcaccacg
     1081 ggtaagcccc acgccttccg ctccgtcatg ctcaagctgg ccggccaaaa cctcgccgag
     1141 gccgaacccc ccgcctgggt ccgcgtcctc ttccaaagcc accccacaat agccgagcga
     1201 gtagccagcg cggaagagaa ttcttaatgt ttaattctta attcttaatt gaggagatag
     1261 gtactgaaac cgtcaattaa gaataaagaa ttaaacatta agaattcgtc cagattgcca
     1321 tttccgggcc gtgccgctat aatctagcaa aatccggggc cggggcggaa gtgttgtata
     1381 gggttgctgt gcgcctccgg attgggtcgt gtctaagtgt gacgttcgca ttagaaagga
     1441 cacatgacta cagacgccgc gccttcgggc cgcataaatc cgctcgctaa tgtgctggac
     1501 tgggacgtga gtgccgcgta cgagcttctg ctcagcctgc gggccatctt cctgcccaag
     1561 gactatggca ttcccgcgcc ctgggccgcc ggcgtgcgca agcgcctctc ccccgccggc
     1621 cagcacgacc tcaagggctt cttcaatccc tccttcgcgg tgattgccta cgcgccaatc
     1681 cacctcgtgc ttgagatgga gcctcccaag acggtcgagc gcttcttcga ttatgtggag
     1741 gcgatacccg gccccgattt catgaggcgg atttacgccc ctaccaagtt ccaggatatt
     1801 cccgccgatg taaaggaccg tgccctggcg ggcaagaagc tgagcgacgc cgacgtggag
     1861 gagtaccgcc ggggcatagc tcgcgcgggc atcggctcta ccccctcggt agccgacgtc
     1921 cgccgcctgt tcgaggacat ggctaacccc gaggccacca agcaacgctg gctcaacgtc
     1981 atgcgcgagt accaggcggt cttcttcgcc gatgaggaga aacgcctgct gcccatcctt
     2041 gagcggatgc tggacgacgc gaaggcccta gcccagaaga ccaccgtgcc cgacctcata
     2101 gagcgcctgt ccaacggctt tacgctcagt cccgatatag agcttgaccg cctggtgctg
     2161 gtcccctcgg tgtggctcca cccctacgtg ataagggccg agatttcgga gagcgagttg
     2221 ctggtggcct ggggggcgca ccctccgggg cacagggtcg tgccgggcga gtcggtcccc
     2281 gaggacgccc tgctggtgct gcgcgccctg gccgacccca cccgcctccg gttgctgcgc
     2341 ctcatcgccg ccgagccgag gtcgctccag tccctcgccc aggaggtcaa gctcagtctg
     2401 cccaccgtat cgcaccacat ccgcgagcta cgcggcgcgg gcctcattcg cctggaagtg
     2461 ggagggcggg gccgcgaaag caagtacacc gtgcgctggc ccagcgcgga gcgagccttt
     2521 gacgacctaa agcagttcgt gctcaccgaa acgtgatgcg gtctgctcta tacgggtcgt
     2581 gaatatgagg ctgcttctgc ggcaggaccg agtcacccat tgtcattctg agcgtagcga
     2641 agaatctcgt ccctcagcac tcaaacagcc cctttccttt agggagcgct tgcattcaag
     2701 aaaggtgtta caccttcgtg gaggacgaga ttcttcgctt cgctcagaat gacaatgggg
     2761 gagaggggca tgccgtacct acgcagccgc attcacgcac gctcttgagc aagccgcctc
     2821 gcccctcacg ttccacgctt caggaccacc ggcccgccct cctctccctc atcctcctcg
     2881 ccgccctcta cctggcggcc ctcgtctcga tgccgcctga cgggctgacg cagcacgacg
     2941 cgggggccaa gtacctgcaa gtacgcaacc tgcgcctcgc cccctccggc ctcgactggt
     3001 cgatcaacta ccccgcccgc cccctcgacc cggagctgca atttgtgccg ttccgcgaca
     3061 ggcagcacta cattgacggc gagagtatct acctgcaatg gcctatcttc ctggggctgc
     3121 tcacgcgcat accctggaag gtcctgggct tctgggggct gtacgtggtg cccctgctgg
     3181 ctgggctggg cacgctgtgg gccgcttaca tactcgctct gaaggcaggc gtcaagcctc
     3241 gcctggcctg ggctgccatc ccactgctcg gcctcgccac ccccctctct atctactcgc
     3301 tcctcttttt cgagcataca ctggccgcct tgctggtcac gctcagcctc ctctcaggcg
     3361 tcgtggccct gtcgtccgag cggcctgctt cccgcgcgct ctggctgaac gccgccctcc
     3421 tcgcggtcgc catctacttc cgctccgagc tatacgtgct ggccctggtc acggggctgg
     3481 cttacgcgta catggcctgg cgcgacgggg cctggcgggg ggcgctggtg cggggggtag
     3541 gggcgttcgc ggtgtcgctg gtgcccctgt gggccttcta cgccatcacg gagggcacga
     3601 tactccccct gcacgccatc tggtatttcc agggcagcga cgcctcctct tcgggcgggc
     3661 tggagcttcc ggcggtgcgc tacctggcct cggccggctg gcgggtggtg cccgatttcc
     3721 tctttggccc ccagtccttc ccatcctctc cgcgtttccc cttgtgggcc gaattggcag
     3781 gcctgctggg gctggtgctg tgcgcgctac ccggcatggc gaggctgcta cggccgcggg
     3841 gcaccatagg ccggtggcgg atgcccgttg tgacggtagg gctgctgctc gtggcgatac
     3901 cgtcgctgtt
//
LOCUS       DASRWZ010000501        12676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_109296, whole genome shotgun sequence.
ACCESSION   DASRWZ010000501 DASRWZ010000000
VERSION     DASRWZ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 12676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12676
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109296"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..1996)
                     /locus_tag="VFH60_07795"
     CDS             complement(302..1996)
                     /locus_tag="VFH60_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313721.1"
                     /translation="MTTSTLPRPRSEQPPSAFARLASRYPRTVAAFARMEGGERALDS
                     VLALERRWLELNSGAARPDPFHSVPTANDLPQCAPQDHYDVAIAGGSLGLVAGVALAQ
                     RGLKVLVFDQGKVGAAHREWNISRRELAALSSWGIFTPHELAQTIATEYRQGVISFDA
                     RGTGGGACPLYLEGVLDVALDAQAVLDLARKRFLRAGGTILEGRSFRRLHMARTGSVA
                     SVFEVEGPAGAEFYRARLAIDTMGSVSPIAVGLNGGPPFDGVCPTAGTIVEGLVADPG
                     LGDVLVSVGGATEGRQLIWEGFPGRRGETTVYLFYYDRTGPRRAKEQGLLDLFEQYFA
                     QLPRYKQSGEGARHIRPVYGYIPARHGRPSRSSARGLLCLGDSAAGQSPLTFCGFGSF
                     VRHLGRVSALVSYALAKGLLEERHLSAISPHQANLRVAWVFSRLMQPWSAADPQCVNR
                     LMNVFCGALEELGTTTTLRFLQDRDTFSEYLRIMLATARRHPAVFPLVARVLGPAGLL
                     KWLGDMAAFASDDLFRAFFLLIGPGRWLRMERLATRVSPGLRLRLAARRTLWRSIE"
     gene            complement(1986..2576)
                     /locus_tag="VFH60_07800"
     CDS             complement(1986..2576)
                     /locus_tag="VFH60_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313722.1"
                     /translation="MQLAAGQYRRLRVDSRDLAARHAPRPWMQDLWLWILLVGPVVAP
                     VFALTGQPLLQLIADGIYMLGNAVCPKVDFHFMFLGQPLAVCSSCWAAVFGLWTVRLL
                     YGRAGEGLGLFARLNLSRFWSRWEATPLEFKLGVLALGFVPWAIDVMLTDTRIWFSPH
                     PVMMLAGYLGGLVAAMLLFPARARMRARLQRLGLDD"
     gene            complement(2580..3989)
                     /locus_tag="VFH60_07805"
     CDS             complement(2580..3989)
                     /locus_tag="VFH60_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6313723.1"
                     /translation="MKTIVVGAGLAGLTAAADLADAGAEVLVLEKRHVPGGKASSWSE
                     GEYPVESGLHIFFGCYRELLGMMKRVGAYDHIHWKEHTIHVAQPGGKVSMFRFPNVPA
                     PFNGIVAFTGNDLLTWREKLTNVRALIRPWLTPLRRVARWDDRTYAQWHRDHGIAEGV
                     LTKWWNPIALSMGFLDAEEMSARPMTTVFHHFSRKADASRVGFLDGPPGERMHRQFVE
                     YLEARGGEVRCDSKVEQLELDWGSGRPTVSGVRLATGEVLQAGAVVLAAPLHNTRRLV
                     PKVLRHHPYFANLWRLKSVPVMNVQVWFDRYVSEVDNLYFTAHAPFSVFADLAVTSPL
                     FDRTGGSLVSMAVAPARKLWHLSDEEIVGACVDALAELWPRTQLARIVHSTVVKIPNS
                     IYREVPGSDALRPTQRTPIRNLALAGDYTRQDYMASMEGAVRSGHRAAGALVTQLGAA
                     GSLRAGKIGAHHLALGRER"
     gene            complement(3986..4723)
                     /locus_tag="VFH60_07810"
     CDS             complement(3986..4723)
                     /locus_tag="VFH60_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6313724.1"
                     /translation="MDGQVPIDNLYVGYSAEGHSQPTGKKEARTANHLDFKLAPQPDK
                     QELSTWGDDTLMEHIARGNTHAFEELYDRYVRGCFGLAMKIVREPTMAEEVVQDVFMK
                     LWSRPGIFSSDRGKFSGWLLTLVHNRSVDKLRRLKAGAAGPVLPLDGTLDSDLNLADV
                     LADTAATPYDQAWTEEQGRIVREAIKALPHPQREAIVLAYFGGLTQKEIAERLEEPLG
                     TVKTRTRAALQQLRRALAGGGLMGEAL"
     gene            complement(4834..5955)
                     /locus_tag="VFH60_07815"
     CDS             complement(4834..5955)
                     /locus_tag="VFH60_07815"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HET6313725.1"
                     /translation="MESNAAWPYPVVRPAARTRFAVPAETHLHPPASLPVYERLAEYL
                     TPPHNLAAARAGKLSRGSGYDAPGSPCLAGAQRPAPDKLWAYRACTEIIKHHSKSFYF
                     SARLLPAAKRAGIMALYAFCRLSDDLVDEADAQGDTHAARLKARADLDRWSRLCDAGS
                     DRAHPVVQAWLYTRVRHGIPRQLPEELLAGIRMDLSIDRYETWDDLWVYCYRVASTVG
                     LMSMYITGTETMEAVLYAVQLGVALQLTNILRDVGEDALSGRIYLPAEDMARFGYSED
                     MLLTGTVNRNFIRLMQFEMARAHALYNSAWRGIALRPADSRPAIAAAATVYRGILHKI
                     VGLRYDVFNNRAHLSMGEKARSLPRVWWQARRMRNRTAQ"
     gene            complement(5945..7375)
                     /locus_tag="VFH60_07820"
     CDS             complement(5945..7375)
                     /locus_tag="VFH60_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6313726.1"
                     /translation="MADVYGRLRATAGKQVVIAGAGLAGLSAAKYLADQGFNITLLEK
                     RAVAGGKVSSWQDADGDWLESGLHVFFGAYHNLLAFLRETGLDDALTWMPHALTFSRP
                     GGALSPLAFPARVPAPFHGLTAIARNRGVLTNPDKVRTGLGLLWPILGNQSYIDRQDD
                     LTYEQWHVRHGLSRRSIADFFDTMSLALNFSKSNEVSAKLLLTVLSHFGKETGASRVA
                     FLKGPPETRLFRPLLSRLRARGVDVRFNAKVKQVRYDPAEGVVEGFELADGTTVEGDI
                     YVSAMPAHNLWKALPIPMRETESLAGLRHLHGVPVMTVQLYFDRPVTGVKNLVFSSRS
                     HISVYAELGQICPDFYEGEGGRSLLELVVAPAARWFKLPDREVVAHVMEEFVDRHPVA
                     AQARLVKSTVVRIPQSVYKATPGMDRLRPDQATGIPNFFVCGDFTRQEYLASMEGAVL
                     SGRRVANRVMQSSTGVSGGREVALGV"
     gene            complement(7624..8580)
                     /locus_tag="VFH60_07825"
     CDS             complement(7624..8580)
                     /locus_tag="VFH60_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6313727.1"
                     /translation="MLFRGRNTTTAPGALMVLLSAVAFGLMPVFAKLAYAEGVNLNTL
                     LAIRFSLASASIWAIWAVAGRRTWARTEVAAGRGAGLVLPLVALGALGYVGQSFSYFT
                     ALNEISASATGLLLYTYPIMVTLLAFFVYREPLTRRKLFALALASVGALLVLGIVSSL
                     LGMGGPGLGALRPSGVAWALAAAAVYSLYIIAGTRLTRDVSPLFASAIIITSAAAVYL
                     LWGALSGTLRFDFAAPGWAWALAIALVSTTLAITAFFVGLPETGPSRAAIISTLEPAV
                     TVFSAALVLGESVSPEQLLGGAFILVSVLVLQYRARQVSPQT"
     gene            8981..10159
                     /locus_tag="VFH60_07830"
     CDS             8981..10159
                     /locus_tag="VFH60_07830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6313728.1"
                     /translation="MFQEIAEILTHNIDEITRRWVEGLRQSQRTEVQKHMLTAEIVDG
                     IKAILANLAQAIETREAPEHETLPLFLISGTDSASVPAAESRKQRGTRPLGGPFGWAQ
                     QSAASQGKQRHSQDYELHEVLIEWIMLRQIVWDVLRVEAAVHGLAVPVEFVQYFDRLL
                     DEMSLASLENYYNASVRDLEKRAIHDPLTQLYNKDYFRQRLNEELRRAVRYAEPLTVA
                     MIDMDGLKPINDTYGHPVGDAVIQAVGFAIRNSSRQPDVPCRYGGDEFAVILPETNKV
                     QARVMSERILRALQNASVVVTQTGQAAATADLISGTTEALSTTNLPLVVSVPTVSIGL
                     AAFPDDARNPEMLIAKADAALYQAKRGGRNRIFAAGDPLVQKGKPREDTTEDERRQEG
                     "
     gene            10137..11468
                     /locus_tag="VFH60_07835"
     CDS             10137..11468
                     /locus_tag="VFH60_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6313729.1"
                     /translation="MSDGKKAEEALKEHNSTVKATGTDEKDADGADPANGGEVRSLHV
                     GVEVNRMKVPSVVLATAGVLLTLFIAWALYSMLHILLLLFIAILLGTAIEPVVNWLRR
                     GPFNRSTGILIVYSGLFIVIALIGWLTLPVILQQVGALGNSLTVTATQMHDEVEKWDP
                     GVIRQQGLLFSEALQAIGNQIGSPEQDSTTSLGEDEKVAAAAHTAVIVAEIFFAFITI
                     FVVAFYWLSERTLIKRAIMSWLPASRANRVRRVWDDIEVKVGGWVRGQLFLMALVGAL
                     SAVGYFFMGIQYWPALALFIAIAEAIPLVGPYIGTAPAVLVALTQPGGGFEKALMVVA
                     FAIVLQTIEGNVLVPRVMKNSVGISPLTVIMSILFGATLAGLAGALVAVPLAGALQVV
                     ISDLKAARESEEKLEEVTAAAEQTRAEAGELVVAKPEDGDTKTRLGAGAQV"
     gene            11472..12632
                     /locus_tag="VFH60_07840"
     CDS             11472..12632
                     /locus_tag="VFH60_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET6313730.1"
                     /translation="MAETLDLSRTSPEVVLHSERLAHARAQLRGHGLDFLVVGPSADL
                     YYLTGIQNRPSERLTVLLLPQEGPAHVVLPAFEAPSLPPLPPDVQVVPWGESDNPARL
                     VASLVAGPTGHPGGAHYTVGASDRLWSVFLLRLQVELPRAMFTPAGSVLSELRQVKSP
                     AELALLEAAGAASDRVFATIVTRPFAGRTELEVSAEIAALLKAEGVQGESFAIVGSGP
                     NSASPHHHAGSRVIEQGDAVVLDFGGPLHGYYCDITRTVFVGSPPESNSEAERVYNLV
                     QAAQEAAVRAALPGVTCEALDAVARDLFAEAGYAQYFMHRLGHGIGLDGHEPPYLVQG
                     NATTLQAGMSFSIEPGLYLPGRFGVRIEDTVYLTPQGAVRCNHAPRDLTVVQ"
BASE COUNT         2280 a         4001 c         4246 g         2149 t
ORIGIN      
        1 ctggaaacca tcgtgttctc ctctgaacag cgtagcacgc ggcagttacc ggggcaatta
       61 tatcagatgg gggagtctgt gaccatcagc gcgaggtgcc gcgtgatagc cctgtggggg
      121 tgccctgtgg ctgaagccac gggcttacaa gaggcaaagt cccctacggg gactaaccaa
      181 catgagggta gtctccgaag gagacttcgc tacttcccag cccgtggctt tagccacagg
      241 gcgtccatgc ggggtgtccc cctgcgcaat aaagattccc taccgcctaa cccatatgcg
      301 ctcactctat ggagcgccag agggtgcgcc gggccgccaa ccgcagacgc agccccggcg
      361 agacgcgggt ggccagcctc tccattctca gccagcggcc cggccctatg agcaagaaga
      421 acgccctgaa gaggtcgtcg gaggcgaacg cggccatgtc gcccagccac ttgagcaggc
      481 cggcagggcc tagcaccctc gcgacgagcg gaaagacggc ggggtggcgt ctcgccgtgg
      541 ccagcatgat gcgcaggtat tcactaaagg tgtcgcggtc ctgaaggaag cgtagggtcg
      601 ttgtagttcc aagctcctcg agcgcgccac aaaagacgtt catcagccga ttcacgcatt
      661 gcgggtccgc ggcggaccag ggttgcatga gcctgctgaa gacccaggcc acgcgcaggt
      721 tggcctggtg ggggctgata gcgctaaggt ggcgctcctc tagcagcccc tttgcaaggg
      781 cgtaagagac gagtgcagac actcgcccca ggtggcggac gaacgaaccg aaaccgcaga
      841 aggtgagcgg cgactgcccg gccgccgagt cgcccaggca gagcagcccc ctggccgacg
      901 acctgctggg cctaccatgc ctcgccggga tgtagccgta tacgggcctg atgtggcggg
      961 caccctcccc ggactgcttg tagcggggca gctgggcgaa gtactgctcg aagaggtcga
     1021 gcaggccctg ctcttttgcg cgacgggggc ctgtgcggtc atagtagaag aggtacaccg
     1081 tcgtttcgcc gcgcctgccc ggaaagccct cccagatgag ctgccgccct tcagtcgcgc
     1141 cccccacgct caccagtacg tcgcctaagc cagggtctgc gacaagcccc tccacgatgg
     1201 taccggcggt ggggcacact ccgtcgaagg gcgggccgcc gttcagcccg accgcgatgg
     1261 gggacaccga gcccatcgtg tcgattgcca ggcgggcgcg gtagaactcg gcccctgccg
     1321 gtccctctac ctcgaacacg gaggccacgg agccggtgcg cgccatgtgc agacgcctga
     1381 acgacctgcc ttccaggatg gtgccccccg cccggaggaa gcgtttgcgc gccaggtcga
     1441 gcacggcctg cgcatcgagg gccacatcga gcacgccttc caggtaaagg gggcacgctc
     1501 caccacctgt acccctcgca tcgaaggaga tgacgccctg ccggtactcg gtggcgatgg
     1561 tctgcgcaag ctcgtgcggg gtgaaaatac cccagctgga gagcgccgcc agttcgcgcc
     1621 gggagatgtt ccactcgcgg tgagcggccc ccaccttgcc ctggtcgaac accagcacct
     1681 tcaatccgcg ttgggcaagg gccacacccg ccacaagccc caggctgccg cccgctatcg
     1741 ccacatcata gtggtcctgc ggcgcgcatt ggggtaagtc gttcgcggtt ggcacagagt
     1801 ggaacgggtc ggggcgcgcg gccccgctgt ttagctccag ccagcggcgt tcgagcgcaa
     1861 ggacgctatc gagggcgcgt tctccacctt ccatccgcgc aaaggccgcc accgtcctgg
     1921 ggtagcggct cgccagccgg gcgaacgccg agggcggttg ctcgctccga gggcgtggca
     1981 gcgtgctagt cgtcaaggcc gagcctttgg aggcgggcac gcatccgggc gcgggcggga
     2041 aagagcagca tcgcggcgac caggccgccg agataaccgg cgagcatcat gaccgggtgc
     2101 ggactgaacc agatgcgcgt gtcggtgagc atcacgtcga tagcccaggg gacgaagccc
     2161 agcgcgagca cgcctagctt gaactctaag ggggtggcct cccatcgcga ccagaagcgc
     2221 gacaggttga gccgagcgaa aagccccaac ccctcgcccg ctcgcccgta gagcagccgc
     2281 accgtccaca ggccgaacac ggccgcccag cacgacgagc ataccgccag gggctggccg
     2341 aggaacataa aatggaagtc caccttgggg cacaccgcat tgcccagcat gtagatgccg
     2401 tcagcgatca gttgcaacaa gggctgcccg gtgagggcga acaccggcgc gacgaccggc
     2461 cccaccagca gaatccacag ccagaggtct tgcatccagg gtcgtggcgc gtggcgggcg
     2521 gcaaggtcgc ggctatcgac ccttagccgc ctgtattgtc cggctgcaag ttgcatggct
     2581 tacctctctc tcccgagcgc caagtgatgc gccccaattt taccagctcg caagcttccg
     2641 gcggccccaa gttgcgtgac gagcgcgccc gccgcccggt ggccgctacg caccgcccct
     2701 tccatcgagg ccatgtagtc ctggcgggtg taatcgcccg cgagcgcgag gttgcgtata
     2761 ggcgtgcgct gcgtggggcg aagtgcgtcg ctgccgggca cctcgcggta aatcgagttg
     2821 ggtatcttca ccacggtaga atgcacgatg cgggccagtt gtgtgcgggg ccacaactcg
     2881 gccagggcgt cgacgcacgc gccaacaatc tcctcgtctg acaggtgcca gagcttgcgg
     2941 gccggggcca ctgccattga cacgagcgag ccgcccgtcc ggtcgaacag ggggcttgtg
     3001 acggccaggt cggcaaaaac ggagaagggt gcgtgcgccg tgaagtagag attgtctacc
     3061 tctgacacgt agcggtcgaa ccagacctgc acgttcatca cgggcacgga cttcagccgc
     3121 cagaggttcg cgaagtatgg gtgatgcctg agcacctttg ggacgaggcg gcgcgtgttg
     3181 tgcaggggcg cggccagcac caccgcaccg gcctgaagca cctcgccggt cgcgagccgt
     3241 acgcccgaaa cggtgggcct gccgctgccc cagtcgagtt ccaactgctc taccttgctg
     3301 tcgcagcgca cctcgccgcc cctggcctcg aggtactcca cgaactgcct gtgcatgcgc
     3361 tcgccgggcg ggccgtcgag gaagcccacg cgggaagcgt cagccttgcg ggagaaatgg
     3421 tggaacaccg tggtcatggg ccgcgcggac atctcctccg cgtcgaggaa gcccatcgac
     3481 agggcgatag ggttccacca cttggtgagc acgccctccg cgatgccgtg gtcccggtgc
     3541 cactgggcgt aggtgcggtc gtcccagcgg gcgacgcggc gcaagggtgt aagccagggc
     3601 cgaatgagcg cgcgcacgtt ggtgagcttt tcgcgccagg tgagcagatc gttgccggtg
     3661 aaggcgacga tgccgttgaa gggggcgggc acgttcggga agcggaacat cgataccttg
     3721 ccgccgggct gagcgacgtg gatggtatgc tctttccagt ggatgtgatc gtacgcaccg
     3781 acgcgcttca tcatgccgag tagctcgcgg tagcagccga agaagatgtg cagccccgat
     3841 tccacggggt actcgccctc ggaccaggag gatgccttgc cccccggcac atggcgtttt
     3901 tccagcacga gcacctcggc cccggcgtcc gcgaggtcgg cggcggcggt cagcccggcc
     3961 agccccgcgc ccacgacaat ggttttcaca gggcctctcc catcaggccg ccaccggcca
     4021 gcgcgcgccg caactgttgc agggccgcgc gggtgcgggt cttgacagtg cccagtggct
     4081 cttcaaggcg ctcggctatc tctttctgag tcagcccacc gaaataggct agcacgatgg
     4141 cctcgcgctg ggggtggggc aaggctttta tcgcctcgcg cacgatgcgg ccctgctcct
     4201 cagtccaggc ctggtcgtaa ggggtggcgg cggtgtcggc aagcacgtca gcgaggttga
     4261 ggtcggagtc cagcgtgccg tcgaggggca ggaccggccc ggcggcaccg gccttgagcc
     4321 tgcgcagctt gtccaccgac cgattgtgca ccagggtcag cagccagccg gagaacttgc
     4381 cccggtccga cgagaatatg ccggggcggc tccagagctt catgaatacg tcctgcacca
     4441 cttcctcggc catcgtaggc tcgcgcacga tcttcatcgc caggccgaag cagccccgca
     4501 cgtagcggtc gtaaagctcc tcgaaggcgt gtgtgttgcc ccgcgctatg tgttccatga
     4561 gggtgtcgtc gccccaggta cttagctcct gcttgtccgg ctgtggtgcg agcttgaagt
     4621 ccaggtgatt ggcggtccga gcctctttct tgccggtagg ttgcgaatgt ccttccgccg
     4681 agtagcccac atacaaatta tctataggta cctgtccgtc catagttctc tcctgtgcca
     4741 tgtcgtgagg tcggcccttg tggcttgtgc tcttgctgtg atgttccgcg tctgtaagta
     4801 cgtctcttgt aagtgtggct tgtactcgcg cagtcattgt gccgtccggt tgcgcatgcg
     4861 gcgggcctgc caccagaccc tcggcaggct gcgggctttt tctcccatgc tcaggtgcgc
     4921 cctgttattg aacacgtcgt atctgaggcc cacgatcttg tgcaggatgc cccggtacac
     4981 cgtggcggcg gccgcaatgg cgggccggct gtcggcgggt cgcagggcaa tccctcgcca
     5041 ggcggagttg tagagggcgt gcgcccgcgc catctcgaac tgcatgaggc ggatgaagtt
     5101 gcggttgacg gtgcccgtga gtagcatgtc ctcgctgtag ccgaagcggg ccatgtcctc
     5161 ggcgggcagg tagatgcggc ctgagagggc gtcctcgcca acgtcgcgca ggatgttggt
     5221 cagttgcagc gccacgccca actgcaccgc gtagagcacc gcttccatcg tctcggtgcc
     5281 ggtgatgtac atactcatca ggcccaccgt gctggcgacc ctgtagcagt agacccacag
     5341 gtcgtcccag gtctcgtagc ggtcgatact caggtccatc ctgatgccgg cgagtagctc
     5401 ttcaggcagt tggcgcggga tgccgtggcg cacgcgggtg tagagccagg cctggaccac
     5461 ggggtgcgcc cggtcgctgc ctgcgtcaca caggcgggac cagcgatcga ggtcggcccg
     5521 tgccttgagc cgggccgcat gcgtgtcacc ttgagcgtcg gcttcgtcca cgaggtcgtc
     5581 actgaggcgg cagaaggcat aaagggccat gataccggcg cgcttggcgg cgggcagcag
     5641 gcgtgcgctg aaatagaagc tcttggagtg gtgcttgatt atctcggtgc atgccctgta
     5701 ggcccacagc ttgtcgggcg cgggtcgttg cgctccggcc aggcaaggcg agccgggcgc
     5761 atcgtagcca ctcccccgcg acagcttccc ggcgcgagca gcggccaggt tgtggggcgg
     5821 cgtcaggtat tcggccagtc gctcgtatac cgggaggctc gccggtgggt gcaggtgcgt
     5881 ctctgcgggc acggcgaacc gcgtgcgggc ggcgggccgc accaccgggt agggccaggc
     5941 tgcgttagac tccaagggcc acctccctgc cgccggacac tccggttgag ctttgcatga
     6001 ccctgtttgc aacccgccta ccgctgagca ccgcgccctc catgctggcg aggtactcct
     6061 gcctggtgaa gtcgccacag acgaagaagt tgggtatccc tgtggcctgg tctggccgca
     6121 agcggtccat gccgggcgtc gctttataga ccgactgtgg gatgcgcacc accgtgctct
     6181 tgacgagcct cgcctgtgct gcaacggggt gtctgtctac aaactcctcc atcacatgcg
     6241 ccaccacttc ccggtcgggc agcttgaacc accgcgccgc cggggcgacc acaagctcca
     6301 ggagggagcg gcccccctcg ccctcgtaga agtcggggca aatctggcca agctcggcgt
     6361 acaccgagat gtgggagcgg ctggagaaaa cgaggttctt cacgccggtc accggcctgt
     6421 cgaaatagag ctgcaccgtc atcacgggta cgccgtgcag gtgccgcagc ccggccagcg
     6481 actcggtctc tcgcatcggt atgggcagcg ctttccagag gttgtgcgcg ggcatggccg
     6541 aaacgtagat gtcgccctcg actgtagtgc cgtcggccaa ctcgaaaccc tcgacaacac
     6601 cttccgccgg gtcgtaccgg acctgcttta ccttcgcgtt gaagcgcacg tccaccccac
     6661 gcgcgcgcag gcgagacagg agggggcgga aaaggcgggt ctcgggcggc cctttgagga
     6721 aggctacacg cgaggccccg gtctctttgc cgaagtggct gagcaccgtc aggaggagct
     6781 tggccgatac ctcgttgctt ttgctgaaat tgagcgcgag tgacatggtg tcgaagaagt
     6841 cggctatcga gcggcggctg aggccgtggc ggacgtgcca ctgctcgtac gtgaggtcgt
     6901 cctggcggtc gatgtaggac tggttgccga ggatgggcca caggaggccc aggccggtgc
     6961 gcaccttgtc ggggttggtg agcacgccgc gattgcgcgc gatggcggtg aggccgtgga
     7021 agggggccgg tacgcgggcc gggaaggcga gtggcgacag cgcgccgccg ggccgggaga
     7081 aggtgagggc gtgaggcatc caggtgaggg cgtcgtccag gccggtctcg cgcaggaagg
     7141 cgagcaggtt gtggtaggcc ccgaagaaga cgtggaggcc gctttcgagc cagtcgccgt
     7201 ccgcgtcctg ccaggacgac accttgcccc cggcgaccgc gcgcttttcc agtagggtta
     7261 tattgaaacc ctggtcggcc aggtacttgg cggcggaaag gccggccagg cccgccccgg
     7321 cgattacaac ttgcttgcct gccgtagcgc ggaggcggcc atatacgtca gccataagag
     7381 agaccttttc tgttccgagc gaactaacta caaagtgtag tgtacaaggc aaattgcgtg
     7441 cctgatgcaa atctcaactt gagtcccgga taaactcagg caccgagacg ttccgcggaa
     7501 agggctgagg accggagccg tgattttcag tggagggcga gcaccacgac tccgttccct
     7561 gcggagctat tctagcatac ggaagaagct ttgtcaatag tttgttcaaa caattcataa
     7621 ggttcaagtt tgaggcgaca cctgccttgc cctgtattgc aggacgagca ccgataccag
     7681 gatgaaggcc ccgcccagca gttgttcggg actgacgctc tcccccagca cgagcgcggc
     7741 cgaaaacacc gtgaccgccg gttcgagggt gctgataatg gcggcgcgtg aggggccggt
     7801 ctcaggcagg cccacaaaga aagcggtaat ggcaagggtg gttgacacca gggcgatagc
     7861 cagggcccac gcccagcccg gcgcggcgaa gtcgaaccgg agcgtgccgg atagcgcgcc
     7921 ccagagcagg tagacggcgg ccgccgaggt gatgatgatc gcgctggcga acaggggact
     7981 cacgtcgcgg gtgaggcggg tgcccgcgat gatgtagagc gagtacacgg ccgccgccgc
     8041 cagcgcccag gcgaccccgc tgggacggag ggcgcccagg ccgggaccgc ccatgcccag
     8101 caacgaggag actataccca gcaccaggag cgcgcccacg ctcgcgaggg ccagcgcgaa
     8161 cagcttgcgg cgggtgagcg gctcgcggta gacgaaaaag gccagcagcg tgaccatgat
     8221 tgggtaggta tagagcagca ggcccgtggc tgaggcggag atctcgttga gggctgtgaa
     8281 gtaagaaaag gactggccta cgtagccgag ggcacccagg gccaccaggg gaagcaccaa
     8341 tcccgcccca cgcccggctg ccacttcggt tcgggcccaa gtgcgcctgc ccgccacggc
     8401 ccaaatcgcc cagatgctgg ccgaggcaag gctgaacctt atcgccaaca gagtgttcag
     8461 gtttacaccc tccgcatagg cgagcttcgc gaataccggc atcaacccga aggcgaccgc
     8521 cgatagcagc accatgagcg cgccgggtgc ggttgtggtg tttctccctc ggaaaagcaa
     8581 cttctcgtct ccgtgtgctg tgtcagaaag gggtagcggc ggtgcgcgga tagcacgccg
     8641 tggggtgcga aggagtacgc ggcgatttct gcaccgacat tataacggcc ggtgcacgca
     8701 tggtaagttc agatggaacg ccctggcgcc ttcgccatgc catgatggcc ctgtggctga
     8761 tgaagttgaa gaaaataccc atcatcgttg ccaatcgacg ggcatcggct ggcggcaatg
     8821 atgggtattt tcttcaactt cattttgaat actggctgcc gtgaaagccg gcattccgag
     8881 cgtgtctgcc ggtcggctga gaggtgctgc tgaaacccgt ggcatgcacc ttgcgtttta
     8941 ctatcattag taggcgcgtt agacagctag tgaattaccc atgtttcaag agatagccga
     9001 aattctcacg cataatatag acgagattac ccgacgctgg gtggagggtc tgcggcagag
     9061 ccaacgcacc gaagtgcaga agcacatgct gacggccgag atcgtggacg gcatcaaggc
     9121 gatcctcgct aacctcgccc aggccattga aactcgcgaa gcccccgaac acgagacgct
     9181 gcccctcttc cttatttcag ggacggacag cgcttcagtg cccgccgccg aatcgcgcaa
     9241 gcagcgcggt acacggcccc tcggtggccc cttcggctgg gcgcaacagt cggccgcctc
     9301 acagggcaag cagcgacact cgcaagacta cgagctgcac gaggtgctca tcgagtggat
     9361 catgctccgg cagatcgttt gggacgtgct gcgcgtcgaa gcggcggtgc acgggcttgc
     9421 ggtccctgtc gaattcgtgc agtacttcga ccggctgctg gacgagatgt cccttgcctc
     9481 gctggagaat tactacaacg cctcggtgcg cgacctcgaa aagcgcgcca ttcacgaccc
     9541 cctcacccag ctttacaaca aggactattt ccggcagcgc ttgaacgagg aactgcgccg
     9601 ggcggtgcgc tacgccgagc ctctaaccgt cgccatgatc gacatggacg gcctcaagcc
     9661 gatcaacgac acctacggcc acccggttgg ggatgccgtg attcaggccg tgggcttcgc
     9721 tatcagaaat tcctctcgcc aacccgacgt gccatgccgt tacggagggg acgagtttgc
     9781 cgttatatta cctgagacca ataaggtgca ggccagggtc atgagcgaac gcatcctgcg
     9841 ggcgctgcaa aatgcgagcg tagtggttac gcagacggga caagccgccg cgaccgctga
     9901 cctcattagt ggcactacgg aggcgctttc gaccacaaac ctgccgctgg tggtgtcggt
     9961 gcccacggta tcaatagggc tggccgcgtt cccggacgac gcccgcaacc ctgagatgct
    10021 tatagccaag gcagacgcgg cactctacca ggccaagcga ggcgggcgca accggatttt
    10081 cgcggcgggc gacccgctgg tccagaaggg caagccaaga gaggacacga cagaagatga
    10141 gcgacggcaa gaaggctgaa gaggccctga aagaacacaa cagcacggtc aaggcgaccg
    10201 ggacggacga gaaggacgcg gacggcgcag acccggcgaa cggcggcgag gtgcgatccc
    10261 tgcacgtcgg cgtggaagtc aaccgcatga aggtaccctc ggtggtgctg gctacggcgg
    10321 gcgtgctttt gacgctgttc atcgcgtggg cgctctacag catgctgcat atcctgttgc
    10381 tgctgttcat cgccatcctg ctggggaccg caatcgagcc ggtggtcaac tggctgcggc
    10441 gaggcccctt caaccggagc acgggcatac ttatcgtcta ttcggggctg tttatcgtga
    10501 tcgccctgat aggctggctc acactgccgg tgattttgca gcaggtaggt gcgttgggca
    10561 actcgctgac agtaacggcg acccagatgc acgacgaagt agaaaagtgg gacccaggag
    10621 tcatccggca gcagggcctg ctcttttctg aggctttgca agccataggc aaccagatcg
    10681 gtagcccaga gcaggactcg actacaagcc tgggcgaaga cgagaaggtc gccgcggcag
    10741 cgcacacggc cgtgatcgtt gccgaaatct tcttcgcgtt catcaccata ttcgtggtag
    10801 cgttctattg gctgagcgag cgcacgctca taaagcgggc tattatgtcc tggctgcccg
    10861 cgagccgcgc caaccgggtg cgccgcgtct gggacgacat agaggtgaag gtcggcggct
    10921 gggtccgtgg gcagttgttc ctgatggcgc ttgtgggcgc gctcagcgcg gtgggctact
    10981 tcttcatggg catacagtac tggcccgcgc tagccctctt catcgccatc gcggaggcga
    11041 ttccgctggt tggaccgtat atcggcaccg cccccgccgt gctggtagcc ctgacgcagc
    11101 cggggggtgg gttcgagaag gccctcatgg tggttgcctt cgcgattgtg ttgcagacga
    11161 tagagggtaa cgtgctggtc ccccgcgtga tgaagaactc ggtgggtatc agcccgctca
    11221 cggtcatcat gagcatactg ttcggggcga cgctggcggg gcttgcgggc gcgctggtcg
    11281 ccgtgccact ggcaggtgcc ctgcaggtgg tcatttccga cctcaaggcg gcccgcgaga
    11341 gcgaggagaa gcttgaggaa gtgacggctg ccgccgagca aaccagggcc gaagctggcg
    11401 agcttgtggt agccaagccg gaggacgggg acaccaagac gaggctgggc gcgggggcgc
    11461 aggtctagcc cgtggcggaa acgctcgacc tgtcgcgcac ctcgcccgag gtggtcctcc
    11521 atagcgagag gctggcccac gcacgcgccc agttgcgcgg ccacggcctc gacttcctgg
    11581 ttgttggccc ctccgccgac ctttactatc tcaccggcat ccagaaccgc cctagcgagc
    11641 ggctcactgt gttgctgctg ccgcaagaag gccccgccca cgtcgtgttg cccgccttcg
    11701 aagcgcctag cttgccaccg ctgccgccgg acgtgcaggt cgtgccgtgg ggcgagagcg
    11761 acaacccggc ccggctggtg gcctccctcg tggcggggcc gacgggccac cctggcggcg
    11821 cgcactacac ggtgggggcg agcgaccggc tctggtcggt gttcctgctg cgcttgcagg
    11881 tggagttgcc ccgcgccatg ttcaccccgg ccggtagcgt gctcagcgag ttgcggcagg
    11941 tgaagtcgcc cgccgagctt gcgctgcttg aggccgcggg ggccgcctcg gaccgcgtgt
    12001 tcgcgacgat tgtcacgcgc cccttcgccg gccgcaccga gcttgaggtg tcggcggaaa
    12061 tcgcggcctt attgaaggcc gagggggtgc agggcgagag cttcgcaatc gtgggctcgg
    12121 ggccgaacag cgcctccccg caccatcacg ccgggagccg cgttatcgaa cagggtgacg
    12181 cggtagtgct cgacttcggc gggccgttgc acggctacta ttgcgacatc acgcgcaccg
    12241 tgttcgtagg ctcgcccccg gaaagcaact cggaggccga gcgcgtatac aacctcgtgc
    12301 aggcggccca ggaggcagcg gtacgcgcgg ccctgcccgg cgtgacctgc gaggctcttg
    12361 atgcggtggc gcgcgacctg ttcgcggaag ccgggtacgc ccagtacttc atgcaccgcc
    12421 tgggccacgg catcggcctc gacggccacg aaccgcccta cctcgtacag ggcaacgcca
    12481 ccacactaca agccggcatg tccttctcca tagagcctgg cctctatctc cccggcaggt
    12541 tcggcgtgcg catcgaggac accgtctacc tcaccccgca aggcgcggtc cgctgcaatc
    12601 acgctccccg agatctcacg gtggtgcaat aagtggcgta gtaaagacgg aaccgccaag
    12661 acgccaagga cgccaa
//
LOCUS       DASRWZ010000502         5115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_726066, whole genome shotgun sequence.
ACCESSION   DASRWZ010000502 DASRWZ010000000
VERSION     DASRWZ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726066"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..2673
                     /locus_tag="VFH60_07845"
     CDS             319..2673
                     /locus_tag="VFH60_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET6313731.1"
                     /translation="MFARLLSLSLALLALSALLLAQAPPSAASPSPSPSQPPQQSPSF
                     ATCYRNYDQVTAFLQSVAAAHPQIATLSDAGPSWEGTRHLWLMRLGSGGGPASEPRPT
                     IFLLAGQHPRDIATIEVLLRLVDHLANNYGLDPNITWLLDNRWIYILALPNPDGYVRA
                     YSDVPGWSKNTNSGNGCGDPFSWGTNLNRNYPYKWNNGGSSNQPCDSTYRGPSALSEP
                     ESRHVLSALQASGRPADLVISLQAPGPSVLYPWGGTSAAPPDAPALDAFAWGLGRLNG
                     TTRANVRRHNPGPANSPTSGILDDTVYGQFDAPAFTLNIGNASAPTCTELEQVWTSQR
                     PALLQAAGAVGTGKAATLSHAFGPEVTQLSIGPGASPGVIQVSAVVSANYGTVAGAVY
                     TIDGNPDGDPTTSMSGSFGAATASVSDQVDTGSLAPGRHLLTVRGKNDADQWGVSSSL
                     FFTTTALPTSTSTSTPVPTDTPALSGSATSTATSSPHTSTPGSTPTATITPIPTFTNT
                     AVATATSTPTEASTSTTTPISAATATTTATPTTTAISATNTATPTRTPTSLGTSTPTL
                     TPPPTSTRTYTPTRTPTYTRTPVPTQTPRPPSTPTPIPCVDFSDVYPSQYFYIPVDWL
                     TCRGIVSGYADNTFRPGNTATRAQIVKMVVLGHTWPLYEPEQPTFSDVGPGDWSYGYV
                     EASVQNGIVGGYADGTFRPNDPVTRGQLCKILVLAREWPLQAPSGASFPDVPPGSPFH
                     PYVETARARGVISGYGDGTFRPSNHATRGQLSKMLYVALTEFGN"
     gene            2914..3495
                     /locus_tag="VFH60_07850"
     CDS             2914..3495
                     /locus_tag="VFH60_07850"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6313732.1"
                     /translation="MEGIWLVSYIVLWLVVLVLIVLVVLLYRQLGIMYLGSAEGVSRD
                     GLEAGAQAPNFSLPDQYGNLQRLADYRGRPLLLLFGSPNCGPCRVLLPELHEWAAEHP
                     EVGVVWLNLASPEENARYASETGATLPMSAHTPGDGLVERFKVRVTPFLFFLDEQGTV
                     RAKGLANTRSALDMYYRQLQAAQAGAPALQRAG"
     gene            3550..4035
                     /locus_tag="VFH60_07855"
     CDS             3550..4035
                     /locus_tag="VFH60_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313733.1"
                     /translation="MFDHKVRLLTEGMARAIGRRKFLTQVGAAVFAGVATLASGRALG
                     GTVLAQQNGGDGRQPPADPFAACKAPNNTFCSVTVPNSSDGCQGAYCFQHFHNSQLLE
                     CHLSYNWGYSVGCWTVMDGGGGGYWTCCDCDCGTTPNAGPSLASCGCARHTNLPMPVS
                     N"
     gene            4060..>5115
                     /locus_tag="VFH60_07860"
     CDS             4060..>5115
                     /locus_tag="VFH60_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET6313734.1"
                     /translation="MSRRPGASILIPLLGLAVAAILAMSTGPTARAGAPPTLAPGTDG
                     ATTQQQNAPAQPYGSGGWALQAAMPGVSVELPDEERFAVKAARIKRAGAAFYPPNGKI
                     YLLGGRMGIDGTDNPVEYYDPGKGPPGAVPYTWLFESTPGAPGTWARKQASIDPCPAS
                     IPNSAKCPGERYTSNMAVATLTDTTGVAIYAIGGSSVNSVPINRVRRYNPVADTMTYL
                     DSDPWHATPARIPGGYAVLNNKLYVFGGFNPRGTGEVYGDTWVFDPMAAAGSKWSQLA
                     DNLSTPRAYIAGVALDGYIYAIGGDTWETNPDVGERRLVPTSVVERMNPLVGLWEAVD
                     SLPQPAGDLGAWAYDAGT"
BASE COUNT          896 a         1844 c         1453 g          922 t
ORIGIN      
        1 ctctgcgtct ctgcggtgaa taatctttat cttcttggcg cgctcttggc gtgcttggcg
       61 gcttggcggt tccgtctcct actttctcct actttggtgc agttattttg tcgcacttcg
      121 caaactcgtt cggaatagtc aatagacact attgacaatt aacggccccg gtgctttaat
      181 acaatactct gtctccccgg caagctggtg taaggcgggc tgctatgtcc gtcctcgccc
      241 aacaggggcc tgtgcagggt aggctcgacg gctgtttgcc cgttagtagt cgctctgtgc
      301 ggctgtcggg ggttacgtat gttcgctcgg ctcctgtccc tgtccctggc gctgctggcc
      361 ctctctgccc tgctgcttgc ccaggccccc ccttccgcgg cctcgccttc gccctcaccc
      421 tctcagccgc cccagcaatc cccctccttt gccacttgct accgtaacta cgaccaggta
      481 accgccttct tgcagtcggt ggccgccgcc caccctcaga tcgccacgct gagcgacgcc
      541 gggccgtcct gggagggtac gcggcacctg tggctgatga ggctcggcag tggcggcggg
      601 cctgcttcgg agccgcgccc caccatcttt ctccttgcgg gccagcaccc acgtgacatc
      661 gccaccatcg aggtgctgct gcgcctggtg gaccacctcg cgaacaacta tggcctcgac
      721 ccgaacataa cgtggctcct cgacaaccgc tggatttaca tcctggccct cccaaacccc
      781 gacgggtacg tccgggcgta ctcagatgtg ccgggctggt cgaagaacac caactccggt
      841 aacggctgcg gcgatccatt ctcctggggc accaacctca accgcaacta tccctacaag
      901 tggaacaacg gtggcagcag caaccagccg tgcgacagca cctaccgtgg cccttccgct
      961 ctctccgagc ctgagagccg gcacgtgctc tcggcgctcc aggcaagcgg caggcccgcc
     1021 gacctcgtta tctccctgca agcgccgggg ccgtcggtgc tctacccctg gggcgggacc
     1081 tccgccgcgc cacccgacgc cccggcgctg gacgctttcg cctggggcct ggggcggctg
     1141 aacggcacta cccgcgccaa cgtccgccgc cacaatcccg gccccgccaa ctctcccacc
     1201 agcggcatcc tggacgacac ggtgtacggc cagttcgacg cgcccgcctt cacgctcaac
     1261 atcggcaacg cctcggcccc cacctgcacc gagctggagc aggtatggac ctcccagcgc
     1321 cccgccctgc tccaggctgc cggcgcggta ggcacaggca aggccgctac cctctcgcac
     1381 gcctttgggc cggaagttac ccaactatcc atcgggccgg gcgcttctcc gggtgtgata
     1441 caggtgagcg cggtagtctc ggccaactat ggcaccgtcg cgggtgcggt ctacactata
     1501 gacggcaacc ccgacggcga tcccacgacc tccatgagcg gaagcttcgg agcggcgaca
     1561 gcgagcgttt ccgaccaggt agataccggc agcctggccc ctgggcggca cctgctgacc
     1621 gtgcgcggca agaacgacgc ggaccaatgg ggcgtgtcgt cctccctctt tttcacgacg
     1681 acggccctgc ccacctccac ctctacctcc acccccgtgc caacggatac tcccgccctc
     1741 tccggcagtg ccacttctac ggctacctcc tcccctcata cgtccacgcc aggcagtacc
     1801 cctacagcta ctattacccc tatccccact ttcacaaaca cggctgtagc cacagctacg
     1861 tctaccccca cggaggcgag cacctctaca accacgccca taagcgcggc cacagcaacc
     1921 acgacggcca cacccacaac gacagccatt tccgccacga ataccgccac tcccaccaga
     1981 acgcccacaa gcctcggtac ttccacaccc acgctcaccc cgccacctac ctctacccgc
     2041 acctacacac ccaccaggac gcccacctat acgcgcaccc ccgtacccac gcagacgccc
     2101 agacctccct ccacgcccac tcctatcccc tgcgtggact tcagtgacgt ttacccaagc
     2161 cagtacttct acatccccgt ggactggctt acctgccgtg ggatagtctc aggctacgcc
     2221 gacaacacct tccggcccgg caacacggcc acccgcgccc agatcgtcaa gatggtggtg
     2281 ctggggcaca cctggcctct atacgagcct gagcaaccca ccttcagtga cgtgggcccc
     2341 ggcgattggt cgtatggcta cgtcgaggcg agcgtgcaga acggtattgt gggcggctac
     2401 gcggacggca ccttccgccc caacgacccc gtgacgcgcg gccagctatg caaaatcctg
     2461 gtactggctc gcgagtggcc gctacaggca ccctccgggg cttccttccc cgacgtaccg
     2521 cccggctccc ccttccaccc ctacgtcgag accgcccgcg cgaggggcgt catctccggc
     2581 tacggcgacg gcaccttccg cccctccaat cacgcaaccc gcggccaact ctccaaaatg
     2641 ctctacgtag ccctgacgga atttggaaat tagaatttcg aattgcgaat ttcaagaaca
     2701 agagctaact ccaaattcga aattcgaaat tctaatttcc aaatccgtaa ttgacattcc
     2761 cccctccccg cccctataat ttcgggtact gggcgaacgc aagggggcca cgcggaattt
     2821 gcgatgctct gcgcccggcc tgtatggttg gggcttagtt gccgtccgtt gtgctctcgg
     2881 tgcgcacggc aggagtgggg cttttccgcg cttatggaag gcatctggtt agttagctac
     2941 atcgtgctgt ggctcgtggt gctggtgctt atcgtgttgg tggtgttgct ttaccgccaa
     3001 ctcggcatca tgtacctggg tagcgcggaa ggggtatcgc gagacggcct ggaagcgggc
     3061 gcgcaggccc ctaatttcag cctccccgac cagtacggca acctccagcg cctggcggac
     3121 taccgtgggc ggcccctgct gctgctgttc ggctcgccca actgcgggcc gtgccgggtg
     3181 ctgctgcccg agctgcacga gtgggccgcc gagcaccctg aagtgggcgt cgtctggctc
     3241 aacctggcct ccccggagga gaacgcgcgc tacgcctcgg agaccggggc taccctgccc
     3301 atgtccgccc acacgccggg cgacggcctg gtagagcgct tcaaggtgcg cgtgacgccc
     3361 ttcctgttct tcctggacga gcagggcacc gtgcgggcca aggggcttgc caacacccgg
     3421 agcgcgctcg acatgtatta caggcagcta caggcagcgc aggccggcgc acccgccctg
     3481 caaagggccg gctgataccc tttagactca aaactcggta gagaaccagc acgtcaggaa
     3541 gcaagcgtca tgttcgacca caaagtaagg ctactcaccg aaggcatggc ccgcgcgata
     3601 ggccgccgca agtttctcac gcaggtgggc gcggcggtgt tcgcgggcgt ggctaccctc
     3661 gcgtcgggcc gcgcgctcgg aggcaccgtg ctggcgcagc agaatggcgg cgacgggcga
     3721 cagccccctg ccgacccctt cgcggcttgt aaggctccca ataatacctt ttgcagcgta
     3781 accgtcccga attcgagcga cggctgccag ggtgcctact gcttccaaca tttccacaac
     3841 agtcagctac tggagtgcca cctctcctac aactggggct actccgttgg ctgttggact
     3901 gtaatggacg ggggcggtgg aggctattgg acctgctgcg actgtgactg cggcaccacc
     3961 cccaacgccg gccctagtct ggcgtcctgt ggttgtgctc ggcacacgaa tcttccaatg
     4021 ccggtatcga actagcttgc tgatggaagg gctatcctaa tgagcagacg acccggcgcg
     4081 tctatcctga ttcccctgct ggggcttgcg gtcgcggcaa ttctcgccat gtccaccggc
     4141 cccacggcgc gtgcaggcgc gcccccgacg ctagcgcccg gtaccgacgg tgccacgacg
     4201 cagcagcaga acgcccccgc ccagccttac ggtagcggcg gctgggcatt gcaggcagca
     4261 atgcccggcg tcagcgtgga gttgcccgac gaggaacgat ttgctgtgaa agccgcgcgc
     4321 atcaagcgtg ccggggcggc gttttaccca cccaacggca aaatctacct cctgggcggc
     4381 cggatgggca tcgacggcac ggacaaccct gtagagtact acgatccggg caaaggccct
     4441 ccgggtgcgg tcccttatac ctggcttttc gagtctaccc ccggcgcgcc cggcacctgg
     4501 gcgcgcaagc aggccagcat agacccttgc ccggccagca tccccaactc ggccaagtgc
     4561 cccggcgagc ggtatacctc taacatggcc gtggcaactc tgaccgatac taccggagtc
     4621 gccatttacg ccataggcgg cagcagcgtc aacagcgtcc ccatcaatag agtgcgccgt
     4681 tacaaccctg ttgccgacac catgacttat ctcgactccg acccctggca cgccaccccc
     4741 gccaggatac ccggcggcta cgcggtgctg aacaacaagc tgtacgtgtt cggtggcttc
     4801 aacccccgtg ggactggtga ggtatatggc gacacctggg tgttcgaccc catggcagcg
     4861 gcgggcagca agtggtcgca gcttgccgat aacctctcca cgcctcgcgc ctacatcgca
     4921 ggggtggccc tcgacggcta tatttacgct atcggcggcg acacctggga gactaacccc
     4981 gacgtcggcg agcgcaggtt agtgccgacc agcgtggtcg agcgcatgaa cccgctggtt
     5041 ggtctgtggg aagcggtaga cagcctgccc cagccggcgg gcgacctcgg cgcgtgggcg
     5101 tatgacgcgg gtacg
//
LOCUS       DASRWZ010000503         1514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_657541, whole genome shotgun sequence.
ACCESSION   DASRWZ010000503 DASRWZ010000000
VERSION     DASRWZ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657541"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFH60_07865"
     CDS             complement(<1..664)
                     /locus_tag="VFH60_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6313735.1"
                     /translation="MEKVRVLIVDDHPLFRQGVRHTLESAKDVEVVGEAPDGQRAIQA
                     AETLAPDIMLVDINLPGLNGLEVTRVVRRRQPQIAVIILTVYEDDEQLFNATKVGAAA
                     YSAKDISPDRLLDIIREVAEGHYPINDTVLSRPFVASRMLNQFRELASTESETGESVF
                     APLTTREVEILDCIARGNSNKEIARILNISDQTVKNHITSILRKLAVTDRTQAVIYAL
                     RHG"
     gene            complement(681..>1514)
                     /locus_tag="VFH60_07870"
     CDS             complement(681..>1514)
                     /locus_tag="VFH60_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6313736.1"
                     /translation="NASLDQLSNRLSWLIHQIDGACAWVLSSGDASELEQGSEGSEGG
                     AQNGASAPSRGLQPSAGEQVMWAQMVMGQEAERARLAREIHDGPAQVLANTVMRLSLV
                     EKMFTHSPGEVQSELERVRVALQESVEDVRRFIFNLRPASLSEIGLLPTLRQYARDYS
                     EQFNVAVELNVPENMHLSSNQELVVFRIIQEALQNIHKHAEATSITINIQQRSGGPLT
                     LTITDNGRGFDPKSVRTASPSSSGLVGMRERAATVGGTLKVESRSGIGTTVTLVLPKV
                     S"
BASE COUNT          249 a          508 c          440 g          317 t
ORIGIN      
        1 acccatgccg tagcgcataa ataacagcct gggtgcggtc ggtcaccgcc agcttgcgca
       61 ggatagaggt gatgtggttc tttacggtct ggtcggagat gttcaatata cgagcgatct
      121 ccttgttgga gttgccccgc gctatgcagt cgaggatttc tacctcgcgt gtagttagag
      181 gcgcgaacac cgactcgccc gtttcagact cggtggaagc aagctcgcgg aactggttga
      241 gcatgcgaga ggccacgaac gggcgcgaca gcaccgtgtc gttgatcggg tagtgcccct
      301 cggccacctc acggattatg tcgagcaagc ggtccggcga gatgtctttg gccgagtacg
      361 cggcggcacc caccttcgtg gcgttgaata gctgctcgtc gtcctcgtac acggtgagaa
      421 taatcacggc tatctgcggc tggcggcggc gcaccacgcg cgtcacctcc aggccgttga
      481 ggccgggcag gttgatgtcc accagcatga tgtccggggc cagcgtctcg gccgcctgta
      541 tggcccgctg cccgtcgggg gcctcgccca ccacctctac gtccttcgcc gactccaggg
      601 tatgcctcac accctgccgg aacagcgggt ggtcgtccac tattagcacg cgtacttttt
      661 ccattcgact ctccctctac ttacgatact ttgggcaaca caagagttac agtcgtgcca
      721 atcccgctcc tactttccac cttcagggtg ccgcctaccg tggccgcacg ctcccgcata
      781 cccaccaacc ccgaactcga agggctggcg gtgcgcaccg atttggggtc gaaaccccgc
      841 ccgttatctg taatcgtcag ggtgagcggc ccgcccgacc gctgctggat gtttatcgtg
      901 atgctggtgg cctcggcgtg cttgtgtatg ttctgcaagg cttcctggat tatacgaaat
      961 accaccagct cctggttgct ggataggtgc atgttctccg gcacgttcaa ctcgaccgct
     1021 acgttgaact gctcgctgta gtcccgtgcg tactggcgca atgtgggcag caggccgatc
     1081 tcgctgaggc tggcggggcg caggttgaaa atgaagcgcc gcacgtcttc cacgctctct
     1141 tgcaacgcca ccctcacccg ttcgagctcc gactgcacct ctccgggcga gtgggtgaac
     1201 atcttctcca ccagcgacag gcgcatcact gtgttggcca gcacctgcgc cgggccgtcg
     1261 tggatctccc gcgccagccg cgcccgctcg gcctcctggc ccatcaccat ctgcgcccac
     1321 atcacctgct cgcccgccga cggttgcagc cccctcgagg gtgccgatgc tccattctga
     1381 gcgccgcctt ccgacccttc cgacccttgc tccaactcgc tcgcgtcgcc ggaagagagc
     1441 acccacgcgc acgccccgtc tatctggtgt atcagccagg agaggcggtt cgatagctgg
     1501 tccaggctgg cgtt
//
LOCUS       DASRWZ010000504         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_40453,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000504 DASRWZ010000000
VERSION     DASRWZ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40453"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VFH60_07875"
     CDS             complement(<1..1216)
                     /locus_tag="VFH60_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET6313737.1"
                     /translation="MDNMRLGPKAADFVWASGIEDSFIPQTRRGHRPLDEYQLIGHYQ
                     HWREDLALAKDLGVNVMRWGVPWYRVEPIPDEYDWRWTDQVLPYMVEELGITPIIDLV
                     HYGCPYWLRREFANEEYHTCVSRYAAAFAERYRGLVRWYTPCNEPLVNSLLCGMQGAW
                     PPYLKGDKGYVKMFVQLAKGILSTVHAIKSVQPEARMVHVEAAGICRAAHPSLEDLAE
                     QNRLKGFLSFDLITGRVTPDHPLYPWLLQSGARRRDLEWIARQPIPVDVMGLNFYPQW
                     STQELNLDEHGHVVSRTVEHDGSGFGAMVEDFYRRYGSPIMITETSARDAEEDRIAWL
                     DSSLRMIKGLREQGVPVLGYTWFPMFTMIEWNYRWERGQLDRYLLDLGIYQLNTTGEG
                     GRWRPFPIAEHLR"
     gene            complement(1643..4078)
                     /locus_tag="VFH60_07880"
     CDS             complement(1643..4078)
                     /locus_tag="VFH60_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313738.1"
                     /translation="MDRTAEMSELIREEAEGGLRMVLQGGTGGRARALLEPLALAPAV
                     GDSPVSGGWDAVTPGEYGLDAEGHTSHQEGTVRFRLHTERLDEPGLFRLVWRVEFADG
                     PFTGAILHTVRLPGVTADASALDLPSLHYGGNTFGTGLFPHPQPGRGFAFRADRLAQP
                     AIHYATPDATWSYFAEDEIPWSPTPDVLYALGIDPLPAGTEGGGLMLFFRYPQIEYAH
                     RGDGSEDAYIAKSTFGPGEDVSATWQPGEVLQKTLYLWLRPPGIRHKYSEPARFLWRR
                     AYLDKRASPPSPPLWWQVGQHVRWFNERLYNPQLGGGQYESPEGSGTAILGFVEQSLR
                     MAANTFVYAGLAIDGADPPLPREDLRTLRDRAAGALTRWATDGLSPEGLLYPVCDAGG
                     FSFGYRDYSDYENLTVVKDEGFDTLRLAGEARDLLLSARSVRMARDGQHESAAASWER
                     AGLSVADWFVSHPLPHGGYASRYKRTGEPLDPYPSGTASVVSLLCDCARLLGDTDPKG
                     SSTYLNAAVEAYEGTLAGLVREGQFGGGTLDASTPDREAAMSALDACILMYEASNDSL
                     YLEDARIAADNLLSYTLVYPIKTFAPDTDAGRQNISTFGASIVSPENQHLDPVSTAPG
                     LLLYGLYAEDDICIQAGAESLKWALDGRWAIREAQGLKQSEQLLHTRWYYNEFFSRRG
                     DYRRGMPLWGRTDSEHGWPQVVPSAALLGCGQVVVDWQTGRAASVDCWQVASSRKDGG
                     SKLYLDLFLCERMGDRELVSVYLRVLRLPRDRKLRVDANGRKAYLTGAQLESGHLLNV
                     PRFDLVRLTIEEE"
BASE COUNT          755 a         1365 c         1353 g          749 t
ORIGIN      
        1 ggcgcaggtg ctccgcgatg gggaagggac gccagcggcc accctcgccg gtcgtgttta
       61 gttggtagat gcccaggtcg aggaggtacc ggtccaactg gcctcgctcc cagcggtagt
      121 tccactcgat catggtgaac atagggaacc aggtgtaccc gagcacgggc actccctgct
      181 cgcgcagccc ctttatcatc ctgagcgacg aatcgagcca ggctatgcgg tcttcctccg
      241 cgtcgcgggc gctcgtctcg gtaatcatga tggggctgcc gtagcgcctg tagaagtcct
      301 ccaccatagc cccgaagccg gagccgtcgt gctccaccgt gcgcgagacg acgtgcccgt
      361 gctcgtccag gttcagttcc tgggtggacc actgcgggta gaagttcaag cccatcacgt
      421 ccacgggtat tggctggcgg gcaatccatt cgaggtcgcg gcggcgcgcc ccgctctgga
      481 gcagccaggg gtacagcggg tggtcgggcg tgacgcggcc cgtgatgagg tcgaacgaga
      541 ggaagccctt gagccggttc tgctcggcaa ggtcctccaa cgaggggtgg gccgccctgc
      601 aaataccggc cgcctcgacg tggaccatcc gcgcctccgg ctgcaccgac ttgatagcgt
      661 gcaccgtgga caggatgcct ttcgccaact gcacgaacat cttgacatag cccttgtcgc
      721 ccttcaaata gggcggccag gcaccctgca tgccgcacaa cagggaatta accagcggct
      781 cgttgcaggg ggtataccag cgcacgaggc cacggtatcg ctccgcgaag gcggcggcat
      841 agcgggatac acaggtgtgg tactcttcgt tggcgaactc gcggcgcagc cagtaggggc
      901 agccatagtg caccaggtcg ataatcggcg tgatgcccag ctcttcgacc atgtagggga
      961 gcacctggtc ggtccagcgc caatcgtact cgtcggggat aggctcgacg cggtaccagg
     1021 gcacgcccca gcgcatcacg ttcacgccca ggtccttcgc cagcgccagg tcttcccgcc
     1081 agtgctggta atggcctata agctggtatt catcgagggg ccggtggcct cgccgcgtct
     1141 gcgggatgaa cgagtcttcg atgccgctgg cccatacgaa gtcggcggcc ttcggcccta
     1201 atcgcatatt gtccatcgct ctttcatcag cctttcgcat ccttgcgcaa cgccctcgtc
     1261 gttgagggac aattgtggac gccccctatg ctgcaagcag gaaagcacct gctctatagg
     1321 agcacgcgta cgggagggcc aagacccggt tttccacact cggaggccct tctaccccta
     1381 tcttattatg accttactgc cgctgtcaag gcggtgggag gttacaatag tcccgactac
     1441 ccgcgggagt ttcggccaga aggtccatgt gattgggaaa gtaccgagtg taagttacgc
     1501 cctgctgtaa gtattacgta tggccgtaac gccggttgct taggtgaagc tccgcctatg
     1561 tcatttctca cttcattcga aatgacatag gcggagcttg gtcctcctga tgcggtacag
     1621 gtacttctca ggctaagtgg ctttattctt cctcaattgt gagcctcaca aggtcgaagc
     1681 ggggcacatt gaggaggtgg ccgctctcta attgcgcgcc ggtaaggtac gccttgcggc
     1741 cgttggcgtc cacacgcagc ttgcggtcgc ggggcaggcg caacacacgc aggtagacgg
     1801 agacaagctc ccggtcaccc atgcgctcgc acaggaacag gtcgaggtac agcttgctgc
     1861 cgccgtcttt gcggctgctc gccacctgcc agcaatccac agaagcggcc ctgcccgtct
     1921 gccagtccac caccacctgc ccgcagccaa gcagcgccgc cgacggcacc acctgcggcc
     1981 agccgtgctc gctgtcggtg cgcccccata gcggcatccc tcgccggtaa tcgccgcgcc
     2041 gagagaagaa ctcgttatag taccagcgcg tgtgcagcag ttgctccgat tgcttgaggc
     2101 cctgcgcctc cctgatagcc catcgcccgt cgagcgccca cttgagcgac tcggcccccg
     2161 cctggatgca aatatcgtcc tcggcataca gcccgtacag cagtaggccg ggggccgtcg
     2221 atacggggtc gaggtgctgg ttctccggcg atacgatgct ggccccgaac gtggagatgt
     2281 tctgcctacc tgcgtcggta tcgggcgcga aggtcttgat agggtagacg agggtgtagc
     2341 tgagcaaatt gtcggcggcg atgcgcgcgt cttcgagata gaggctgtcg ttcgaggcct
     2401 cgtacatcag gatgcaggcg tcaagggcgg acatcgccgc ctcgcggtcg ggagtggaag
     2461 cgtcgagcgt gccgccgccg aactgtcctt cccgcacgag tccggccagg gtgccctcgt
     2521 aagcctctac agcggcgttc aggtaggtgc tactaccttt ggggtctgtg tcgcccagca
     2581 gccgcgcaca atcgcacagg agcgatacca cggacgcggt gcccgaagga tagggatcga
     2641 gcggctcccc tgtgcgcttg tagcgcgagg catacccacc atgcggtaga gggtgcgaca
     2701 cgaaccagtc cgccacgctc aggcccgctc gctcccagga agcagcggcg ctctcgtgct
     2761 ggccatccct cgccatgcgg acggacctgg ccgacaagag caggtccctt gcctcacccg
     2821 ccaacctcaa agtgtcgaac ccctcgtcct tgaccacggt gaggttctcg tagtcggagt
     2881 agtcgcggta gccaaacgag aagccaccgg cgtcacacac cgggtagagc agcccttcag
     2941 gactaaggcc gtctgtggcc cagcgggtga gcgcaccggc cgcgcggtct ctcagcgttc
     3001 tcaggtcttc gcgaggcagg ggagggtccg cgccgtcgat agcaaggccg gcgtagacga
     3061 acgtattcgc cgccatgcgc agcgattgct ccacgaagcc gagtatcgcg gtgccggagc
     3121 cttcgggcga ctcgtactgg ccgccaccca attgcgggtt gtaaagacgc tcgttgaacc
     3181 agcgaacgtg ctggcccacc tgccaccaga gtggcggcga gggaggagag gcacgcttgt
     3241 cgagataggc cctgcgccac aggaagcggg caggctcgct gtacttgtgt cgtattccag
     3301 gcgggcgcag ccacaggtag agcgtcttct gcaacacctc acccggctgc caggtggcgc
     3361 tcacgtcctc gcccggacca aaggtgctct tggctatgta ggcgtcctcg ctaccgtctc
     3421 cgcgatgcgc gtactcaatc tgcgggtacc ggaagaacaa catcagcccg ccgccctccg
     3481 tgccggcagg cagcgggtcg atgcccaggg cgtaaagcac gtccggcgtt ggggaccagg
     3541 gaatttcgtc ctctgcgaag tagctccagg tagcgtcggg cgtagcgtag tggatggcgg
     3601 gctgggccag gcggtcggcg cggaaggcga agccgcgtcc cggctggggg tgcgggaaga
     3661 ggccggtgcc gaacgtattg cccccatagt ggaggctggg caggtcgagg gccgacgcat
     3721 cggcggtcac cccaggcagt ctcaccgtgt ggaggatagc cccggtgaac gggccgtccg
     3781 cgaactccac gcgccagacc agccggaaca agccaggttc gtcaaggcgc tcggtgtgca
     3841 gcctgaagcg caccgtcccc tcctggtgcg acgtatggcc ctcggcgtcc aggccgtact
     3901 cgccgggcgt tacagcgtcc cagccgccac tgacggggct atcgcctaca gccggggcta
     3961 gcgccagcgg ctcaagcagg gcgcgcgccc ggccgccggt gcccccttgc aacaccattc
     4021 ggaggccgcc ttcggcctcc tctcgaataa gctcagacat ctcggccgtg cggtccatgc
     4081 gtcggtgcac tccttcaggc agcaattacc cgccccaatc gcaggggcag cacaaatgag
     4141 gcgacccgga gtcaagcagc aagacgcgcg ccaacgcgca tgcttcggtg cgcctccttg
     4201 atcagttgag cctacagaga ta
//
LOCUS       DASRWZ010000505         5085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_932111, whole genome shotgun sequence.
ACCESSION   DASRWZ010000505 DASRWZ010000000
VERSION     DASRWZ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932111"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFH60_07885"
     CDS             complement(<1..209)
                     /locus_tag="VFH60_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313739.1"
                     /translation="MAISTVALWELRRYAAGKSTWVMFAAVFGFFAFLTWYMRSITLV
                     TYFLPEPAAVAVTGTSAWGMVRTII"
     gene            complement(434..1342)
                     /locus_tag="VFH60_07890"
     CDS             complement(434..1342)
                     /locus_tag="VFH60_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313740.1"
                     /translation="MSAANEHGQPGHPAHLEEVLREQGEPEEDIASLLPVLGSLQQWQ
                     APEPSREDTQRLVELLVPLMLASEGRAPGRALSPVRAAREQLGSSKGAWLGRALDVAY
                     AQVGLLRLQFWVASAFLTVLGMLVLFYNGSIHEATVLRASGPLLAVVGVSAVFRSVRL
                     RTLEIELSCPISPMRLALIRLALVLGYDMVLGLALSLGLWAVREHGPGSDVVTLMLHW
                     LAPLLAVSGLALLFSVRLPTEVAALLAYALWLSVLALSLAEEAPGRLRPLLESGESIL
                     ALAGVVLLALGIARSRAAVPLLLPRS"
     gene            complement(1350..1907)
                     /locus_tag="VFH60_07895"
     CDS             complement(1350..1907)
                     /locus_tag="VFH60_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6313741.1"
                     /translation="MQFQSDEQLMARVMEGDREALARLVERYHSPLIAYLYRLLEGDR
                     ALAEDLTQETFIRVIQQRSYRTGSPFKPWLYAIATNLARDHFKSAATRHAASDDHDFT
                     ARPDESSGPEQRVLAAELHNEVAAALAQLGPEYRATLLLRLFAGLSLAEIAASLDVPV
                     GTVKSRLSVGCHRLRDLLAGNDRKD"
     gene            complement(2052..3440)
                     /locus_tag="VFH60_07900"
     CDS             complement(2052..3440)
                     /locus_tag="VFH60_07900"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6313742.1"
                     /translation="MSEQPTTKPAPLYTYHLIVPHPAETRLLMREEGDGWLLPSFEPE
                     IKYLARVGQNNTWVRERLGLECITLYAAHVEDGTHGTRRVDVTYVLQVRDRDWSPPGG
                     YRWVSRDELAGLEVSVPGQRAVIEAWLDEREGRVEPPQRGAWNAPGWYDEASDCIRAQ
                     LEAHGIEMTAPPAQFKHWIITSALKVPTTHGDYYLKAVPAVFAQEPGITAALAARYPD
                     YVPAPLATRSRPGEGWMLMPDFKGEVLERISVEQISEALRILARMQIDSISRVDELLA
                     AGCPDRRLERLAAEFETLLHDPVVLVGLAPEEIERFQKHMPQFEEMCARLAGYAVPQT
                     LLHGDFHGGNLLARDERILIFDWTDGCIAHPFLDLITGLNNRYPGFNEQEQAAIRDAY
                     LAEWSAYESPEHLREAAKLAWPLGALHQAVSYRGIMATPSDATSWEMAGAAGYFLKHA
                     LKAVEQQAGEER"
     gene            3586..3945
                     /locus_tag="VFH60_07905"
     CDS             3586..3945
                     /locus_tag="VFH60_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313743.1"
                     /translation="MIPLIPRPVHAVMDYLWGIALMFAPEALGFKNDGAANAYCKARG
                     GSMVLTSLMTRYELGLIKLIPFNMHLLIDFVSALSLGFLGPKLFGFEKNKEATQVTML
                     FSAIELGAVLMSKRDKK"
     gene            4216..4692
                     /locus_tag="VFH60_07910"
     CDS             4216..4692
                     /locus_tag="VFH60_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET6313744.1"
                     /translation="MASGPERVQEALDALGLEVTVVRLPDSTRTAPEAARAVGCDVGA
                     IAKSLLFVADGEPVLVICAGDRRVDTERLAGVLGAEHVAMAPAAEVRRVTGYAIGGVP
                     PVGHAVQVRALFDGSLLRWPLIYAAAGAHDALFSVDPHRLLEVSGAEVADVGEAIG"
     gene            4741..>5085
                     /locus_tag="VFH60_07915"
     CDS             4741..>5085
                     /locus_tag="VFH60_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313745.1"
                     /translation="MSGITSAGGPAAGHAHDRALLMAIYRTLLARYGPQGWWPTVTGS
                     RWEVMLGAVLVQRTTWRNADRALANLRSALGSEALSDPAALLDLPDERLVEVLHPAGH
                     FNRKPRTLKLLAR"
BASE COUNT          941 a         1583 c         1672 g          889 t
ORIGIN      
        1 atgattattg tgcgcaccat gccccacgcg ctggtaccgg tcacagctac ggcggcgggt
       61 tcggggagaa agtaagtcac aagggtaatg ctgcgcatgt accaagtcaa gaacgcaaaa
      121 aagccaaaca ccgccgcaaa catgacccag gtagatttgc cagcggcgta gcgccttagc
      181 tcccaaagag ctacggtcga gatcgccaca ggtctggtct cctcagttcc acgggcaaca
      241 cgtatagcta tggtgttgga ctctgctgac atgtgcgtta tctcccttga tgtgttacgt
      301 ccttactcct ggtagcttac gaactgaata cgagacagga gcaaaaaggt tcgcgatctg
      361 ctaccggcgt gaatcgttgc ccggtgttga aggggagcgg ccgccggcaa aacagcttcc
      421 tcggcgtacg cgctcaactg cgagggagca ggagcggcac ggccgcccgc gagcgggcga
      481 tacccagcgc aagtagaaca acaccggcga gcgcgagaat ggattcaccg ctttccagga
      541 gcgggcgcaa gcggccaggt gcctcctcgg ccagcgataa tgccagtaca gagagccaca
      601 gcgcgtaggc cagcagggcg gcaacctcgg tgggcaggcg cacgctgaag agcagagcca
      661 ggccggagac cgccagcagc ggggccagcc agtgcagcat gagcgtcacg acgtccgaac
      721 ccggcccatg ctctcggacc gcccacagcc ccaggctcag cgccaacccc agcaccatgt
      781 catatccaag caccagggcc aggcgaatca gggccagccg catgggtgag atagggcagg
      841 aaagctcgat ttccagcgtc ctcagcctga cgctgcggaa tactgcgctt acccccacga
      901 ccgcgagcaa tggccccgac gccctcaaaa cggtggcttc gtggatggac ccattgtaga
      961 acagcacgag catgccgagg acggtgagga aggcgctcgc gacccagaac tggagacgca
     1021 acagccctac ctgcgcgtac gccacatcga gcgcccggcc cagccaggca cccttgctac
     1081 ttcccagttg ctctcgcgcg gccctgaccg gagacagtgc tcgccctggt gcccggcctt
     1141 ctgacgcgag catgagcggc accagcaact caacgagccg ctgcgtatct tcccgggacg
     1201 gctcaggagc ttgccactgc tgcagcgacc ccaacaccgg cagcagcgag gcgatatcct
     1261 cctccggctc gccttgttcg cgcaagacct cctccaggtg cgctgggtgg ccgggttggc
     1321 catgttcgtt ggcagcgctc ataccctctt cagtcctttc tatcgttccc ggcgagcagg
     1381 tcgcgcaggc ggtggcagcc cacggacagc cgcgacttga cggtgcccac cggcacgtcc
     1441 aacgaagccg ctatttctgc caggctcaga ccggcaaaaa gacggagcag gagcgtcgcg
     1501 cggtactcag gtccgagctg cgctagagcg gcggcaacct cattgtgcag ctcggcggcc
     1561 agtacccttt gctcaggccc gctcgattcg tcgggtcgag cagtgaagtc gtggtcgtcc
     1621 gaagctgcat ggcgggttgc cgccgacttg aaatggtcgc gggccaggtt ggtggctatg
     1681 gcgtacagcc agggcttgaa ggggctgccg gtacggtaac tgcgctgctg aataactcgg
     1741 atgaaagtct cctgggtgag gtcttccgcg agggcgcggt ccccctccaa caagcgatag
     1801 agataggcta tcagcgggct gtggtagcgc tcgacaaggc gggccagggc ctcgcggtcg
     1861 ccctccatga cccgcgccat caactgctca tcgctttgaa attgcaccgc agtcctatcc
     1921 tggtctgtgt tggcggcaac gtcgcgggcc acgcacgctg tacgagatag cgtcggcgaa
     1981 ggttcgcggc acgcttgcaa gtggaagcag ctaccaaccg cgagacgcct gctgggtgct
     2041 tgcgaagcgt ttcaacgttc ctcgcccgcc tgttgctcta ccgccttcag cgcatgctta
     2101 aggaagtagc ctgccgcccc ggccatctcc cagctggtgg catcggaagg ggtggccatg
     2161 atgccgcgat agctgactgc ctggtgcaag gcacccagcg gccaggccag tttagcggct
     2221 tcccgcaggt gttcggggct ttcgtaggcg ctccactccg ccaggtaggc atctctgatt
     2281 gcggcctgct cctgttcgtt gaagcccgga tagcggttgt tcaggccggt aatgaggtcg
     2341 aggaaggggt gcgcgatgca gccgtctgtc cagtcgaata tgaggatgcg ctcgtcccgc
     2401 gccaggaggt tgccgccgtg gaaatcgccg tgtagaaggg tctgtggcac agcgtaaccg
     2461 gccagccgcg cgcacatctc ctcgaactgt ggcatatgct tctgaaacct ctcgatctcc
     2521 tccggcgcca ggcccacaag caccacaggg tcgtgtagca gcgtttcgaa ctccgcggca
     2581 aggcgctcaa gccgccggtc ggggcagccc gccgccagca actcatcaac gcgagagatg
     2641 ctgtcgattt gcatccgggc caggattcgc aacgcctcgc taatctgttc tacgctgatt
     2701 cgttccagca cctcaccctt gaaatcgggc atgagcatcc agccctcacc gggccgcgac
     2761 ctggtagcaa gcggggcggg cacatagtcc gggtagcggg cggcgagcgc ggccgtgatg
     2821 ccaggctcct gggcgaagac ggcgggcaca gccttgaggt agtagtcccc gtgcgtggtc
     2881 ggcaccttga gcgccgaggt gattatccag tgtttgaact gcgcaggcgg agccgtcatc
     2941 tctatcccgt gggcctcgag ttgcgcccga atgcagtcac ttgcctcgtc gtaccagccg
     3001 ggcgcgttcc aggcaccgcg ctgcggaggc tcgacccgcc cctcccgctc gtccagccag
     3061 gcctcgatta cggcgcgctg cccaggcacg ctcacctcca gaccggcaag ttcgtcccgg
     3121 ctcacccacc ggtagccgcc gggtggagac cagtcccggt cgcggacctg caacacatag
     3181 gtcacgtcaa cgcgcctcgt gccgtgggtg ccgtcttcca catgcgcggc gtacagggtg
     3241 atgcactcca ggcccaggcg ctcgcgcacc caggtgttgt tctgcccaac ccgggcaagg
     3301 tacttgatct ccggctcgaa ggagggcaac agccagccgt ccccctcctc tctcattagc
     3361 aggcgtgtct cggccgggtg tgggacgatg aggtggtacg tgtagagggg tgcaggtttg
     3421 gtcgtgggct gttcgctcaa gccggtgctc ctagcgtgga tttggtgaac agtatagcag
     3481 acgctgcgag cgcaaggccc atcgagggcg cagtccgcga gtgggcatac aagttgcttc
     3541 ctggtgagct tgcagcgatt ccagacttct accaggagac acaaaatgat ccctctaatt
     3601 ccgaggcccg tacacgccgt catggactac ctgtggggca tcgcgctgat gttcgcgccc
     3661 gaggccctcg gcttcaagaa tgacggcgcg gccaacgctt actgcaaggc gcgaggcggc
     3721 agcatggtgc tcaccagcct tatgacgcgc tacgagcttg gcctcatcaa gctaatcccc
     3781 ttcaatatgc acctgctaat cgactttgtg agcgcgttga gcctgggttt cctggggcca
     3841 aagctgttcg gcttcgagaa gaacaaggaa gctacccagg tcaccatgct cttctccgcg
     3901 atagaactgg gcgcggtgct catgagcaag cgggacaaga agtagggcgt gccgagtacc
     3961 gagtgctgag caaagagacg gaaacgcaaa gacgcagaga acgcaaagga cgcgcaaaga
     4021 gtaggatggt ttaacttggt gcccttcatc actcggtagg caggggctaa tcattctcgg
     4081 caattcattg gctggacgtc cccaccccag cctgcggaga atgattaagc cctgactcgg
     4141 taggtgtccc tccttgtgag cggcccaaca cgcatgttac actcgattaa ctactatgta
     4201 acgttgggag agtgtatggc tagcgggccg gagagagtgc aggaggcgct ggacgcgctg
     4261 gggctggaag ttaccgtcgt gcggctgccg gacagcacgc gcaccgcgcc cgaagccgca
     4321 cgcgcggtgg gctgcgacgt aggggctatt gccaagtcgc tgttgttcgt agcagacggg
     4381 gagcctgtgc tggtcatatg cgcgggcgac cggcgggtgg acacggagcg gctcgcgggt
     4441 gtgctgggtg ccgagcatgt agccatggca ccggccgcgg aggtaaggcg ggtcacgggg
     4501 tacgctattg gaggcgtgcc gccggtgggc cacgcggtac aggtacgcgc gctcttcgat
     4561 ggcagcctgt tgcgctggcc cctgatctat gcggcggcgg gcgctcacga cgcgctcttc
     4621 tcggtggacc cccaccgcct gcttgaagtc agcggggccg aggtcgcgga cgtcggagag
     4681 gccattggtt gagcgcatcg aggcaccggc tgctgtcggg gcgaaagccg ggcggccaga
     4741 gtgagcggga ttacaagcgc aggagggcct gcggcggggc atgcacacga cagggcgttg
     4801 cttatggcga tttaccgtac cctgttggcg cgctacgggc cgcagggctg gtggccgacg
     4861 gtgacgggca gccggtggga ggtgatgctg ggcgcggtgc tcgtgcagcg gaccacctgg
     4921 cgcaacgcgg accgcgccct ggctaacttg cgctcggccc tggggagtga ggcgctgtcc
     4981 gaccccgccg cgctgctgga cttgccggac gagcggctgg tggaggtatt gcatccggcc
     5041 ggtcacttca acaggaagcc gcgcacgctc aagctgctgg cgcgt
//
LOCUS       DASRWZ010000506         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_110009, whole genome shotgun sequence.
ACCESSION   DASRWZ010000506 DASRWZ010000000
VERSION     DASRWZ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110009"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1415
                     /locus_tag="VFH60_07920"
     CDS             207..1415
                     /locus_tag="VFH60_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET6313746.1"
                     /translation="MIAIKGGRVLTMARDEQGRDIVWEGGTVLLGDDGKIAAVGSADD
                     VTVPEGAEVVDAGGRVVVPGFIDAHTHVGIGEMDIGKEGSDTNENTDPVTPQVRAIDA
                     TFPRDTGFRDARAAGITAVNVMPGSGNVIGGLAVLLKTYGDRIDDMVIRNPSGLKAAM
                     GENPKKSYGDQKKMPSTRLGVAALVRENLVKASNYLLKRDASEMQSDPTKPFERDLKM
                     EHLGMVLRREIPMRWHAHRSDDILTAFRIRDEFGFDMVLDHATEAHLIPDELLKRNIP
                     AVVGPTLTARYKPEVKGRSLTTPGILVREGVKVALTTDHWVVPVQYLPMQLVMAVKHG
                     LDRDQALRLVTINPAEIMGVEDRIGSIAVGKDADVAIWSGDPLDIYQHPVRVYVNGKL
                     VHEHEGPDSY"
     gene            complement(1435..3114)
                     /locus_tag="VFH60_07925"
     CDS             complement(1435..3114)
                     /locus_tag="VFH60_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313747.1"
                     /translation="MRTIPDDAFYYLQTARHIAAGDGSTFDGLHPTNGYHPLWMLTLV
                     PFSVIAQDSVAFTRLALALSILCSLLSAAVFHLLLRRLTTAWWIPPLGAGLYFLNSQA
                     IVSNLNGLETSLSTLFFVALAYLVIPAGQERQTTRQAVLLGLCLGLLFLARTDNAFYI
                     GMFFLVVLVRTWKSEMPASGERAVSPGAGTRGTGIAASRAVARTGATIAVAAAVVAPW
                     LAWNLASFGSIVQSSGLAIPHLLQESYRLEGHTDAEMFQRSVAHFASFFRAGFFNFLG
                     IPAFLFVPAALLCGVVLGYAWRNGRGSLSQQGVRASLEGQSSLRAAIGVVGPLWVAAI
                     LLVFTHTFIRWNPRDWYFDQMIALSALTFCLALIALNPGETIPKALHLALRRLAPGVS
                     EVILRPPMLRRIVAGVGKVAVAVLLLVPLVLGSMRLWNGIYPHQVELLDAAYWLKANT
                     APGEIAAGFNSGIMGFFSDRPVVNLDGVMNNAAYVAITKKDLAAFMEQSQVRYYVDFD
                     PQMVDLYARFMGDAESRVSRVPVAAFERPNIDWYDSVVRAYRLDWLDQGLR"
BASE COUNT          714 a         1009 c         1030 g          533 t
ORIGIN      
        1 cggagactgg ggaacttgag ggtagtctcc gaaggagact ttgtctctgg taagcccgcc
       61 actttagtgg cagggcacac ccgcagggcg accagcgtgg ctaccttggc tctattctct
      121 gcccggtggc acaggctttg ataacgcccc aggagtgcta caacggacca acttgctaca
      181 ttttacgcat accgggaggt agtttcatga tcgcaatcaa gggtgggcgc gtgctgacca
      241 tggcgcggga cgagcaaggg cgcgacatcg tgtgggaggg cggcaccgtg ctgctgggcg
      301 acgacggcaa gatcgcggcg gtcggctcgg cggatgatgt gaccgtgccc gaaggcgcgg
      361 aggtcgtgga cgctggcggg cgcgtggtgg tgcccggctt tatcgatgcc cacacccacg
      421 taggcatcgg cgagatggac atcggcaagg aaggctccga caccaatgag aacacagacc
      481 ccgtgacccc gcaggtgcgc gccatagacg ccactttccc ccgcgatacc ggcttccgcg
      541 acgcccgggc ggcaggtatc accgccgtga acgtcatgcc cggcagcggc aacgtcatag
      601 gcggcctggc cgtgctgctc aagacctacg gggaccgcat agacgacatg gtcatcagga
      661 acccctcagg gttgaaagcc gcgatgggcg agaaccccaa gaagagctac ggcgaccaga
      721 agaagatgcc cagcacccgc ctgggcgtgg cggccctcgt gcgcgaaaac ctcgtcaagg
      781 cgagcaacta cctgctcaag cgcgacgcca gcgagatgca gagcgaccct acgaagccgt
      841 tcgagcgcga cctcaagatg gagcacctcg gtatggtgct gcgccgggag atacctatgc
      901 gctggcacgc ccaccgtagc gacgacatcc ttaccgcctt ccgcatacgc gacgagttcg
      961 gcttcgacat ggtgctggac cacgctaccg aggcccacct gatacccgac gagctgctca
     1021 agcgcaacat ccccgccgtg gtcggcccca cgctcacggc acgctacaag ccggaggtga
     1081 agggccgcag cctcaccacg cccggcatac tggtgcgcga gggcgtgaaa gtggccctca
     1141 ccaccgacca ctgggtcgtg cccgtgcagt acctcccgat gcagcttgtc atggccgtaa
     1201 agcacggcct cgaccgcgac caggcgctcc ggctcgtgac gatcaacccc gccgagatca
     1261 tgggtgtgga ggaccgcata ggctccatcg ccgtgggcaa ggacgcggat gtggcgatat
     1321 ggtcgggcga cccgctcgac atctaccagc accccgtgag ggtgtacgtc aacggcaagc
     1381 tggtccacga gcacgagggg ccggacagct attagtcccg cccggcgctg caacctacct
     1441 caagccctgg tcgagccagt cgagcctgta cgcgcgcact accgagtcgt accaatcgat
     1501 gttggggcgt tcaaaagccg ctacgggcac ccgcgagact ctgctttccg cgtcgcccat
     1561 gaaccgcgca tagaggtcca ccatctgggg gtcaaaatca acgtagtacc ggacctgcga
     1621 ctgctccatg aaggcagcca ggtccttctt tgtgattgct acgtaggcgg cgttgttcat
     1681 taccccatcc aggttgacca cgggtctatc cgagaagaac cccataatcc ccgaattgaa
     1741 ccctgccgca atctcaccgg gtgcagtgtt tgctttcagc cagtaggcgg cgtcaagcaa
     1801 ctcgacctga tgcgggtata tgccgttcca caggcgcata ctgcccagta cgagagggac
     1861 tagcagcaag accgctacgg caactttccc caccccggca acgatgcgtc tcagcatggg
     1921 cgggcgcaaa atgacttctg agacacccgg tgccaggcgg cgcagagcga gatggagggc
     1981 cttgggtata gtctcgccag gattcaaggc aatcagggcc aggcagaagg tcagggcaga
     2041 gagcgcgatc atctggtcga agtaccaatc gcgcgggttc caccggatga aggtgtgagt
     2101 gaataccagc aagatggcgg cgacccaaag cggtcccaca acccctatag ccgcgcgcaa
     2161 tgagctttgg ccctcaaggg acgcacgcac gccctgttgc gagaggctgc cccgcccgtt
     2221 tcgccaggcg taccccagca ccaccccgca aagcagggca gcaggtacga aaagaaacgc
     2281 gggtataccg aggaaattga agaagccggc gcgaaagaag gaggcaaagt gggccacgct
     2341 acgctgaaac atctcagcat cggtgtggcc ttccagccgg tacgactcct ggagcagatg
     2401 gggtatggcg aggccgctac tctggacgat tgacccgaag ctcgccaggt tccaggccag
     2461 ccacggcgct acgacggcgg ctgccacggc aattgtggct cccgttctcg ctacggccct
     2521 gcttgctgct atccccgttc cacgcgtccc cgcgcctgga cttacagccc tttccccgga
     2581 cgccggcatc tcagacttcc acgttctcac taacacaacc aggaagaaca tccctatata
     2641 gaaggcgttg tcggtgcggg ccaggaagag cagccccagg caaagcccta gcagcacggc
     2701 ttgccttgtg gtctggcgtt cctgcccggc gggaatgaca aggtaagcca aggcaacgaa
     2761 aaatagcgtg ctgaggctcg tctcaaggcc gttgaggttc gaaacgatgg cctgtgagtt
     2821 caagaagtaa agccccgccc ccaatggcgg tatccaccac gcagttgtaa gccgcctcag
     2881 aagcaagtgg aacacggccg ccgaaagcag gctgcagagg atgctcagcg ccagggccaa
     2941 ccgggtgaaa gcgaccgagt cttgagcaat caccgagaag ggtacgagcg tgagcatcca
     3001 cagggggtgg tagccgttgg tcgggtggag gccgtcgaag gtagagccat ctccggcggc
     3061 tatatggcgg gcggtttgca agtagtagaa cgcgtcgtcc ggtatggtgc gcacaagcgt
     3121 ttcctgattg gcgaacagga cacccaggcg cggcaccatg cccatgatga gtagaagaag
     3181 agttatcctg gctgagtgcg acatggtgag caagcgatgg actacgggac taaggcgaac
     3241 ggtgaaagct gatgttgtgc tcaccaatta tatatgagct tgtaaa
//
LOCUS       DASRWZ010000507         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_658036, whole genome shotgun sequence.
ACCESSION   DASRWZ010000507 DASRWZ010000000
VERSION     DASRWZ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658036"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2314
                     /locus_tag="VFH60_07930"
     CDS             <1..2314
                     /locus_tag="VFH60_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_271413140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET6313748.1"
                     /translation="AGTEQPEPASAPEEQGTYSWSKTSGAHAQGEPERDPTLQHPNCV
                     LNILRRHYARYTPEMVADVCGCTVEQFERVAEAMMANSGRERTAAIVYAVGWTQHTTG
                     VQISRTAALLQLLLGNAGRPGGGVMAMRGHASIQGSTDIPTLYNLLPGYIPHPTSARG
                     HNSLAEFLEKETVPKGYWANLPKFVVSLLKAWYGDAARVDNEWGFGWLPRISGDHSQL
                     PTFARMAEGKVKGLFLLGQNPAAGAPNSRLNREGLRRLDWLVVRDFFLLESATFWKEG
                     PDNPDPKAIGTEVFFLPAAAGAEKPGTLTNTQRMVQWHDKAVDPPGDCRSDLWFMWNL
                     GRRLKQLYAASTLQRDQGLLNLTWDYQQDEPEVLPDGTRSRIQDEPSAEKVLKEINGY
                     GESTEYRVPSSEAEGEEDSVLGTRHSVLLRGFEELKDDGTTACGCWIYCGIWPEEHRN
                     RAAERPPLNPQSGIPNPNFAWAWPGNRRMMYNRASADPDGRPWSERKKYVWWDEQLGR
                     WTGFDTPDFPPDKRPDYRAAPDDVGMEAISGDSPFIMHSDGKGWLFAASGVKDGPLPT
                     HYEPLESPVDNLLYRQQANPTAKVEASPLNPMALPVDPQYPVVATTYRLTEHYLSGGM
                     SRFNSWLNELQPAMFVEMSPELAEERGIEHGDWVVISSPRGEIEARAMVTPRLHTLKV
                     GGRVVHQVGVPIHFSFTGEVTGGQANELIPVVTEPNVSMHEGKAFMCQLRKGRLVTPS
                     DVPTVPVAKRPSEAPMPDTPQQAQPEGRSA"
     gene            2373..2762
                     /locus_tag="VFH60_07935"
     CDS             2373..2762
                     /locus_tag="VFH60_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6313749.1"
                     /translation="MDKARVARWLDGYVAAWKSYDPQAIGDLFAEDARYFYDPFNRPV
                     EGREAIVAAWLAEPDEPGTYDASYEPVIVEGNRAMAVGRSIYYEDDGTTVNAEWSNVF
                     LLTFDDDGRCSQYEEWYFEKPEKLDGE"
BASE COUNT          575 a          890 c         1000 g          454 t
ORIGIN      
        1 cgcgggcacg gagcagccgg agcctgcgag cgcgccggag gagcagggca cctattcgtg
       61 gagcaagact tcgggcgcgc atgcccaggg cgaaccggag cgtgacccca cgcttcagca
      121 ccccaactgc gtgctcaaca tcctccgcag gcactacgcg cgctacacgc cggagatggt
      181 ggccgacgtg tgcgggtgta cggtagagca gttcgagcgg gtggccgagg cgatgatggc
      241 gaactcgggg cgcgagcgga cggcggctat cgtgtacgcg gtggggtgga cgcagcacac
      301 gacgggggtg cagataagtc gtacggcggc gctgctacaa ctgctgctgg gcaatgcggg
      361 gaggccgggc ggtggggtca tggcgatgcg cgggcacgcc tcaatccagg ggtccaccga
      421 catccccacg ctgtacaacc tgttaccagg ctacatcccg catcccacct cggccagggg
      481 gcacaactct ctcgcggagt tcctggagaa agagacggtg cccaagggct actgggccaa
      541 cctgccgaag ttcgtggtga gcctgctgaa agcctggtac ggggacgcgg ccagggtcga
      601 taacgagtgg ggcttcggct ggctgcctcg tatctccggc gaccactcgc agctacccac
      661 gttcgcgcgc atggccgagg gcaaggtgaa ggggctgttc ctgctggggc agaacccggc
      721 ggcgggcgcg cccaactcgc gcctcaaccg ggagggcctg cgcaggctcg actggctggt
      781 ggtgcgcgac ttcttcctgc tggagagcgc taccttctgg aaggaaggcc ccgacaaccc
      841 cgaccccaaa gccataggca cggaggtctt tttcctcccg gcggcggcgg gggcggagaa
      901 gccgggcacc ctcacgaaca cccagcgcat ggtgcagtgg cacgacaaag cggttgaccc
      961 gccgggcgac tgccgctccg acctgtggtt catgtggaac ctgggccgcc gcctcaagca
     1021 gctttacgcc gccagcactc tacaacgcga ccagggcctc ctcaacctca cttgggacta
     1081 ccagcaggac gagcctgaag tcctcccgga tggcacacgc agccgcatcc aggacgagcc
     1141 aagcgccgag aaggtgctga aagagatcaa cggctatggc gagagtaccg agtaccgagt
     1201 tccgagttcc gaggcggaag gggaagaaga ctcggtactc ggcactcggc actcggtact
     1261 tctccggggc tttgaggagc taaaggacga tgggaccacc gcctgcggct gctggatata
     1321 ctgcggcatc tggcccgaag agcaccgcaa ccgcgccgcc gaacgcccac ccctaaatcc
     1381 gcaatccgga attccaaatc ccaatttcgc ctgggcctgg cccggcaacc ggcgcatgat
     1441 gtacaatcgc gccagcgcgg acccggacgg ccggccgtgg agcgagcgca agaagtacgt
     1501 ctggtgggac gagcaactgg ggaggtggac cggcttcgac acgcccgact ttccgcccga
     1561 caagcgtccc gactaccgcg ccgcgcccga cgacgtgggc atggaggcga taagcggcga
     1621 ctcgcctttc atcatgcatt cggacggcaa aggctggctc ttcgcggcct ccggcgtcaa
     1681 agacggcccc ctgcccacgc actacgagcc tctggagtcg ccagtggaca acctgctcta
     1741 caggcagcag gccaacccga ccgccaaggt ggaggcttcg cccctcaacc ctatggccct
     1801 gccggtggac ccgcagtacc ctgtcgtagc caccacttac cggctgaccg agcactacct
     1861 gagcgggggc atgagccgct tcaatagctg gctgaacgag ctgcagcccg ccatgttcgt
     1921 ggagatgagt ccggagcttg cggaggagcg ggggatagag cacggcgact gggtggtaat
     1981 cagcagcccg cgcggtgaga tcgaggctag ggcgatggtc accccgcgcc tgcacacgct
     2041 caaggttggg gggcgagtgg tgcaccaggt gggggtgccc atccacttca gctttacggg
     2101 cgaggtcacg ggcgggcagg cgaacgagct tatacctgta gtgacggagc cgaacgtaag
     2161 catgcacgag ggcaaggcgt tcatgtgtca gttgcgcaag ggccgcctgg ttacgccgtc
     2221 tgacgtgccg acggtgccgg tcgctaaacg gcccagtgag gcaccgatgc cggacacgcc
     2281 ccagcaggcg cagccggaag gtagatcggc gtgagggggc gaatttggaa ttgcggatat
     2341 cgaatttcgg actttgcgtg aggagttgaa taatggataa agcaagggtg gcccggtggc
     2401 tggatggcta cgtcgcggcg tggaagagct acgacccgca ggcgattggc gacctgttcg
     2461 cagaagacgc caggtacttc tacgaccctt tcaacaggcc ggtggagggg cgggaggcga
     2521 tagtcgccgc gtggcttgcc gagcctgacg agccgggcac ctacgacgcg agctacgagc
     2581 cggtcatagt cgagggcaac agggcgatgg cggtggggcg cagcatctac tacgaggacg
     2641 acggcaccac cgtgaacgcc gagtggagca acgtcttcct gctgaccttc gacgacgacg
     2701 gccgttgcag ccagtacgag gagtggtact tcgagaagcc ggagaagctg gacggggagt
     2761 gacgagtgac gagtaacgag tgatgaggac tgaggactga gtggccttag ctctaaccgt
     2821 tatctacttt gcgccttttg cgcttctatc tcttaactca gtcctcagtc ctcatcactc
     2881 attactggtc aataagtgag gtagagcgca tgagccagg
//
LOCUS       DASRWZ010000508         1842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_110121, whole genome shotgun sequence.
ACCESSION   DASRWZ010000508 DASRWZ010000000
VERSION     DASRWZ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110121"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VFH60_07940"
     CDS             <1..995
                     /locus_tag="VFH60_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6313750.1"
                     /translation="AKQAGVSRSTVSRVMNDHPNVDQETRARVRSVAESLNYQPNVAA
                     RSLAAGRTHILGLVIPMGVSALFSDPYFPLLIQGIASACNANEHSVMLWLAEPEYERR
                     MIRQVLQGGLIDGVILASALMDDPMLEALRKRGLPFILVGRHPTDNEVSYVDVDNVNS
                     AREMVAYLLRLGHKRVATIAGPNNMIAGADRLQGYLLALRNRGVASDPALIVEADFTE
                     EGGYIAMQRLVPHAPDAVFVASDAMAVGALRALREAGLRVPEDIAMAGFDDIPFAARA
                     DPPLTTVRQPIQRMGTLAAETLIDLISHPQPQPRRIILPTELLIRESSGAPIGR"
     gene            1217..>1842
                     /locus_tag="VFH60_07945"
     CDS             1217..>1842
                     /locus_tag="VFH60_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313751.1"
                     /translation="MSLSKSRIVWLAVLTLVILPLLAACGGTTDTPTAVPAATNTSAP
                     PVAAATDTAPAEADPTEAEAAPTDTAAPVAAVSPTVPPTVLAGGEGCAAGATKLTWYI
                     GLGAGADADVIPKEKAWVDNFNKSQTEACLTMQAVHNPESYDALKAQLAAGTAPDIVG
                     PVGKAGRASFQGSWADVGQLAAGANHDLSQYDPALLEFTKDEGVLVGIP"
BASE COUNT          347 a          663 c          538 g          294 t
ORIGIN      
        1 tcgccaagca ggccggggtg tcgcgctcca ccgtttcccg cgtgatgaac gaccacccca
       61 acgtggacca ggagacgcgg gcgcgcgtgc gctcggtcgc cgaaagcctg aactaccagc
      121 ccaacgtggc cgcccgcagc ctggcagccg ggcgcaccca catcctcggc ctcgtcatcc
      181 ctatgggcgt gtccgccctc ttctccgacc cctacttccc cctcctcatc cagggcatcg
      241 cctccgcctg caacgccaac gaacactccg tcatgctctg gctggccgaa cccgaatacg
      301 agcgccgcat gatacgccag gtgctgcaag gcggcctcat cgacggcgtc atactcgcct
      361 ccgcgctcat ggacgaccct atgctcgaag cgttgcgcaa gcgcggcctg cccttcatcc
      421 tggtgggccg ccaccccacc gacaacgaag tcagctacgt ggacgtggac aacgtgaaca
      481 gcgcccgcga gatggtcgcc tatctgctgc gcctggggca caagcgggtc gccacgattg
      541 ccggtcccaa caacatgata gcgggggccg accgcctgca gggttacctg ctcgcgctac
      601 gcaaccgtgg ggtcgcctcc gaccccgccc tgattgtcga ggccgacttt acggaagaag
      661 gcggctacat agccatgcag cggcttgtgc cacatgcgcc tgatgccgtg ttcgtcgcca
      721 gcgacgcaat ggcggtgggg gcgctacgcg ccctgcgaga ggcgggcttg cgcgtgccgg
      781 aggacatcgc gatggccggg ttcgacgaca taccctttgc cgcccgcgcc gacccacccc
      841 tcaccaccgt ccggcagccc atacagcgta tgggcacatt ggccgccgag acattgatcg
      901 acctgataag ccacccccag cctcagcccc gccgcatcat cctgcccact gagttactca
      961 tccgggaatc aagcggtgcc cccatcggcc ggtaagccgc cgcaagcata gttgcccgct
     1021 tggacctacc gttcgcgaac ggtacagcga tagagccaaa cattacgtct gaatatggtc
     1081 agcccgcgtt tcttcaacct tcgattacca tgttcacgag ccaaaaggag gtggtagcac
     1141 actaagacca ggcttgcaag tgttgtttca taacactgac cacgaagtgt tctgacccca
     1201 aaggagggta aagaaaatgt cactgtcgaa atcacgcatc gtatggttgg ccgtcctgac
     1261 cctggtaatc ttaccgctac tggccgcgtg cggaggcacc accgacaccc cgactgccgt
     1321 ccctgccgcc accaatacgt ccgcgcctcc tgtggccgcc gccaccgata cggcccccgc
     1381 cgaggcagat cccaccgagg ccgaggctgc ccccaccgat acggcagccc ccgtagcagc
     1441 ggtatctccg accgtcccgc ccacggtact tgctgggggt gagggctgcg cagccggcgc
     1501 gaccaagctg acctggtaca tcggcctcgg cgcgggcgct gacgctgatg tgatcccgaa
     1561 ggaaaaagcc tgggtggata acttcaacaa gtcgcagacc gaggcctgtc tgacgatgca
     1621 agctgtccac aaccccgaat cgtacgacgc gctcaaggcg cagctcgccg cgggcacggc
     1681 tcccgacatc gtcggcccgg tcggcaaggc aggacgcgcc agcttccagg gttcctgggc
     1741 ggacgtgggc cagttggccg cgggcgcgaa ccacgacctg agccagtacg acccggcgct
     1801 gctcgagttc accaaagacg agggcgtgct cgtcggcata cc
//
LOCUS       DASRWZ010000509         3073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1343186, whole genome shotgun sequence.
ACCESSION   DASRWZ010000509 DASRWZ010000000
VERSION     DASRWZ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343186"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFH60_07950"
     CDS             complement(<1..388)
                     /locus_tag="VFH60_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET6313752.1"
                     /translation="MPGKTNTPRIEALLSARLFMSPQIVGEHIYFISNLSGRHSLYRM
                     RQGGSVPEPLLPPDIALNDPSHVNGYSFYVFPRLGKIMVMLDNNGDEVYQPVLVPLEG
                     GFPEPAFGSELEGYQVFALKCFADRNL"
     gene            complement(502..1188)
                     /locus_tag="VFH60_07955"
     CDS             complement(502..1188)
                     /locus_tag="VFH60_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6313753.1"
                     /translation="MAEQAQTVAQEFQPADRLTITSLETVKVLSDPLRLQILEQLLGQ
                     PATVKQLAAELKIPQTKLYYHINMLEEHGLVRVVSTRVVSGIIEKQYWVTAYSIDLDR
                     TLFASPGGKGISEEVNSLLQTMFDGVRIDLNKSVEAGLVDLASLTNDQEREASRMVLV
                     RALAQVPEDRVEELYKKMHELVKEFHACDSDSPDAQTHGLMVAMFPTTYKLPSKKSRK
                     PTGDAAKTLD"
     gene            1629..>3073
                     /locus_tag="VFH60_07960"
     CDS             1629..>3073
                     /locus_tag="VFH60_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313754.1"
                     /translation="MESEAGGTRRPLRRVVVVFKTHFDLGFTGLPHEVMRQYTGPIFS
                     AVRALILATGGEPAHLRYNWTLPAWPLKFLLHDPSVPAETREQARRLVEEGRLHWHAW
                     PFTTHTAFCGLEELVRGLHVSRALAEEFGRRPGGAKQTDVPGHTWATPSLLHGAGVRF
                     LHLGANSGSHAPHVPRLFWWEGPDGSRLLTYYSPGGYGTPPLPPQDWPFDTWLALQQT
                     VDNHGPQSPGDLLRLRRTVEQGAPEAELVFGQLGDFYDSLLEHPEQLDGLPVVPYDLA
                     DTWIHGVGSMPREVARVRSLRHKLLKLEVIATLLDWPGEGNGHSAEFALSHGIAPEID
                     AAYEHLLLFGEHTWGLDVKSTIARVFDEGFSAARLTEPYRRVESSWAAKADYVTKAEE
                     AYARAYARVLDAWHDHFERLRQKREQDPLWQPYNREFEEHQVRPAGSEEDSQAPEEVV
                     FIPPPVPAGKNVLETDSLRIEVDPASGGITS"
BASE COUNT          538 a          941 c          990 g          604 t
ORIGIN      
        1 ccaggttgcg gtccgcgaag cacttgagcg cgaacacctg gtaaccctcc agctcgctgc
       61 cgaacgcagg ctcagggaac ccgccttcga gcggcacgag cacgggctgg tagacctcgt
      121 cgccgttgtt gtcgagcatg accatgatct tacccagcct ggggaagacg tagaaggagt
      181 agccgttgac gtgtgagggg tcgttgaggg cgatatccgg gggcagcaga ggctccggga
      241 cgctgccgcc ttgccgcatg cggtagaggc tgtgccttcc gctcaggttg ctgatgaagt
      301 agatgtgctc gcccactatc tgcggcgaca tgaacaggcg cgccgacagc agggcctcta
      361 tacgtggggt gttggttttt ccgggcatgg ttatatctcc tgtgtgaaag ggtgcgcgtg
      421 gccctggtgt accgcttcac cggggcttac agtgtgcttg gtttttcagt aacgcagggc
      481 cgtgacgtgg gataggccgg gctagtcaag tgttttggcc gcatcgccgg tgggcttcct
      541 gcttttcttg gaagggagct tgtaggtcgt cgggaacatc gccaccatca agccgtgcgt
      601 ctgggcgtcg gggctatcgc tgtcgcacgc gtggaactct ttgaccagtt cgtgcatctt
      661 cttgtacagc tcctcgactc tgtcctccgg cacctgggcg agcgcgcgca ccaggaccat
      721 ccggcttgcc tcgcgctcct ggtcgttggt cagactggcg aggtctacca ggcccgcttc
      781 gacgctcttg ttcaggtcga ttcgcacgcc gtcgaacatt gtttgcaaca gactattgac
      841 ctcttcgctg atgcccttac ctccgggcga ggcaaacagc gtcctatcga ggtcgatgct
      901 gtaggccgtc acccagtact gcttttctat aatgccggag accacccgcg tgctcaccac
      961 cctgacgagg ccgtgctctt ccagcatgtt aatatgatag tagagcttgg tctgagggat
     1021 cttcaattcg gcggctagct gcttgaccgt ggcgggctgc ccgagcaact gctccaggat
     1081 ttgcagcctg agggggtcgg aaagcacctt caccgtctcg aggctggtga tagtgagccg
     1141 gtcggcgggc tggaactcct gggcgactgt ttgagcttgc tcggccattc tacctccgta
     1201 gaccattcaa taatattcaa atgaatgata aagcatgaat ggtagcctgt caatgggtcc
     1261 gggccaaatt gccccgccaa ttttcgcccc cttgaccgcg tatccctcca cccttagaat
     1321 ggcaactagc cgcacgcatg tgcaggcaaa gcacggccgt gacttagttg gacggcggga
     1381 ctctgttccc cttgtcatcc tgaacgtagt gaaggatcag gtgggtagtt agaccctccc
     1441 aagtgtcgga ggagggtggt aagggctact atggacccat gttgggagac tcaagaatgg
     1501 gtggctgatc cttcactacg ttcaggatga caaggggaac atggtcgtac agccccgcaa
     1561 tcggggtggg acgtaacagc gaacccaacg ggcacttttc acgaaacgga tgggcgagga
     1621 ggcagcactt ggagagcgag gcaggcggca cacgaaggcc cctccgcagg gtggttgtgg
     1681 tcttcaagac ccacttcgac ctgggcttca cgggcctacc ccacgaggta atgcggcagt
     1741 acaccgggcc gatcttctcg gcggtgcgcg cgctcatcct ggctaccggg ggcgagcctg
     1801 cgcacctgcg ctacaattgg acgctgcccg cctggcctct caagttcctc ctgcacgacc
     1861 cctccgtgcc cgccgagacg cgcgagcaag cccgccgcct ggtggaggag gggcggctcc
     1921 actggcacgc ctggcccttc accacgcaca ccgccttttg cgggctggag gagcttgtgc
     1981 gcggcctgca cgtctcgcgc gcgctggcgg aggagttcgg gcgccggcct gggggggcca
     2041 agcagaccga cgtgccgggc cacacctggg ccacgccctc gctgctgcac ggggcggggg
     2101 tccgtttcct ccacctgggg gccaactcag gcagccacgc gccccacgtg ccgcgcctct
     2161 tctggtggga ggggccggac ggctcgcggc tgctgactta ctactcgccc ggcggctacg
     2221 gtactccccc cctcccgccc caggactggc cgttcgacac ctggctggcc ctgcaacaga
     2281 cggtggacaa ccacggcccg cagtcgccgg gggacctgct gcgtctgcgc cggaccgtgg
     2341 aacagggcgc gcccgaagcg gagcttgtgt tcggacagct aggcgacttc tacgattcgc
     2401 tgctcgaaca ccctgagcaa cttgacggct tgccggtggt gccctacgac cttgcggata
     2461 cgtggataca cggggtgggc agcatgcccc gcgaggtggc gcgggtgcgg agtctgcggc
     2521 acaagctgct gaagctggag gtgatagcta cactcctgga ttggcctggg gagggcaacg
     2581 gccactcggc tgagttcgct ctgtcgcatg gcatcgcccc tgagatagac gccgcctacg
     2641 agcacctatt gctgtttgga gagcacacct gggggctgga cgtaaagagc accatcgcac
     2701 gcgtattcga tgagggcttc agcgccgccc gcctgaccga gccgtaccgc cgcgtggagt
     2761 cttcctgggc cgctaaagca gactacgtaa cgaaggccga agaagcctac gcgcgcgctt
     2821 atgccagggt gctcgacgcc tggcacgatc attttgagcg gctgcgtcag aagcgggagc
     2881 aggaccccct ctggcagccc tataaccggg aatttgagga gcaccaggta agacctgccg
     2941 ggtctgagga ggacagccaa gcccccgaag aagtggtttt catccctccc cctgttcccg
     3001 ccggaaaaaa cgttcttgag accgactcgc tgcgtatcga ggttgacccg gcgtcgggtg
     3061 gtataacctc gtt
//
LOCUS       DASRWZ010000510         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_658181, whole genome shotgun sequence.
ACCESSION   DASRWZ010000510 DASRWZ010000000
VERSION     DASRWZ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658181"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..2029
                     /locus_tag="VFH60_07965"
     CDS             152..2029
                     /locus_tag="VFH60_07965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET6313755.1"
                     /translation="MTLDSNVRATDEPHSNAENIVDEVAASVARMGKIGSCMLPSFSP
                     DGGRIAFVSNLSGVPQVWTVPTAGGWPELVTALDDQVLKVEWSPDGEWLAFWLAPGGG
                     LNQQIYVVRPNGMDLLRLTEGGQVNNWLERWTHDGKWLTVASNRRTPDTMDAYLLDPR
                     SGEWRFVADTGGIGQLTDVSRNGRHAVLYRMKNRGDDDLYLVDLESGRETLLTAHTGP
                     GSFDHGRFSPDGNTVYMSSNKDRERRAFARVSIGEDGRAGEIELLAGRDDAELEYLEV
                     SEDGATAALVWNAGGRSELAFFDLRSLQETPGPALPAEVVISIAFSRDARYLALTLTG
                     AASPNDIWVLERQSSKLWQVTHSPHAGVELDRLPRPELVSFQAHDGLELSGWLYRPRA
                     GQSPGPTVLSFHGGPEAQERPLFNTTYQALLSQGIAVFAPNVRGSDGFGKTFVNLDNG
                     VLRVNAVRDIEACVRYVVQAGVAYPGRIGITGGSYGGFMTLSGLAEYPDTFAAGAIIC
                     GVVNFETFFAQTEPWMAAISKTEYGDPDTEAEMLRDLSPIHKIDRVKGATIVLHGAND
                     TNVPVVEAEQVVDNLKRRGVPVDYVLYPDEGHGFRKIPNRIHSTVAITRWFVEHLKAR
                     Q"
BASE COUNT          400 a          677 c          660 g          345 t
ORIGIN      
        1 ggtgctgttg gacgatattc ttcgctacgc tcagaatgaa aaaggcgaga tagggtccaa
       61 atgtccagct tggacacagc ccttacgagg ggccacttac accactactc attagtattc
      121 tgacccttgt ccgacttgtt aggagattat catgaccctt gactccaatg tgcgcgccac
      181 agacgaacca cactcaaatg cagagaacat tgtggacgaa gtcgccgcct ccgtagccag
      241 gatgggcaag atcggaagtt gcatgctgcc cagtttctcc cccgacggtg ggcgtatcgc
      301 cttcgtgtcc aacctgagcg gcgtgcccca ggtgtggacg gtgcccacag cgggcgggtg
      361 gccggagctg gtgaccgcgc tcgacgacca ggtgttgaag gtggagtggt cccccgacgg
      421 ggagtggttg gccttctggc tcgcgcctgg tggcgggctg aaccagcaaa tatacgtggt
      481 ccgccccaac ggcatggacc tgctcaggct caccgaaggc gggcaggtca acaactggct
      541 cgaaaggtgg acgcacgacg gtaagtggct cactgtcgcc tccaatcgcc gcacccccga
      601 cacgatggac gcttacctgc tcgacccgcg ctccggcgag tggcgcttcg tggccgacac
      661 gggcggcatc ggccagctta ccgacgtgag ccgcaacggc cgccatgccg tgctctacag
      721 gatgaagaac cggggcgacg acgacctcta cctggtggac ctggaaagcg gacgtgagac
      781 cctgctgacc gcgcacacag gccccggcag cttcgaccac ggcaggttct cccccgacgg
      841 caacaccgtg tacatgtcgt ccaacaagga ccgcgagcgt cgcgctttcg ccagggtgag
      901 tataggcgaa gatgggcggg caggggagat agagctgcta gcggggcggg acgacgccga
      961 gttggagtac ctggaagtca gcgaggacgg cgcgaccgcc gcgctcgtct ggaacgccgg
     1021 gggccgtagc gagctggcct tcttcgacct gcgcagcctg caagagaccc ccggacccgc
     1081 cttgcccgcc gaagtcgtaa tcagcatcgc cttttcccgc gacgcgcgct acctggccct
     1141 gaccctcacc ggcgcggcct ctcccaacga catctgggtg ctggaacggc agtcgagcaa
     1201 gctgtggcaa gtcacccaca gcccgcacgc gggggtcgag ttggaccgcc tgcctcggcc
     1261 cgaactggtg agcttccagg cgcacgacgg gctggagcta agcggctggc tctacaggcc
     1321 ccgtgctggg cagtcgcccg gcccgactgt gttaagcttc cacggcgggc ctgaggccca
     1381 ggagcggccc ctcttcaaca ccacctacca ggccctcctt tcccagggca tagcggtgtt
     1441 tgcccccaac gtgcgcggct cagacggctt cggcaagacc ttcgtcaacc tcgacaatgg
     1501 ggtgttgcgg gtgaacgccg tgcgcgacat agaggcttgc gtacgctacg tcgtgcaggc
     1561 aggcgtggcc tacccgggtc gtatcggcat cacgggcggc tcctacggcg gctttatgac
     1621 gctgtcgggc ctcgctgaat atcctgatac gttcgccgca ggtgcgatca tctgcggagt
     1681 ggtcaacttc gagactttct tcgcgcagac cgagccatgg atggccgcca tatcgaagac
     1741 cgagtacggc gaccccgata cggaggccga gatgctgcgc gacctctcgc ccatacacaa
     1801 gatagaccgc gtcaaggggg ccactatcgt gctgcacggt gccaacgaca ccaacgtgcc
     1861 cgtggtcgag gccgagcagg tagtggacaa cctcaagcgc cggggcgtgc cggtagacta
     1921 tgtgctctac cccgacgagg ggcacggctt ccgcaagata cccaaccgca tccactccac
     1981 cgtagccatc acccgctggt tcgtggagca cctcaaggct cgacagtagc agtcagagac
     2041 gaaaccgcca agcatgccct gagcgtagcc gaagggacgc ca
//
LOCUS       DASRWZ010000511         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1069549, whole genome shotgun sequence.
ACCESSION   DASRWZ010000511 DASRWZ010000000
VERSION     DASRWZ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069549"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..893)
                     /locus_tag="VFH60_07970"
     CDS             complement(21..893)
                     /locus_tag="VFH60_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013332787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313756.1"
                     /translation="MKIILSRKGFDSAAGKVASPILPNGQLCSLPIPGGESNRPIRYA
                     DIMAGDISLGTLVGDLTSGKRKLEKIGPDAEAHLDPDLRYSSIPREAGWKPIFGQDGA
                     AESHLQRQGVSAGDLFVFFGWFRQTELAEGHYRYVKDAPDCHYIFGWLQVERRIPVAN
                     RRELPSWSLYHPHCSREQHSRLDSLYVSTDRLQLPDMDGSLPGGGVFTMSDDRLRLTC
                     PGGLRSTWQLPGWFYPQEGLPRLSYHTNANRWSLQADHTLLSTVGRGQEFVLDCDHYP
                     QATEWLAGILKLAA"
     gene            complement(969..>2329)
                     /locus_tag="VFH60_07975"
     CDS             complement(969..>2329)
                     /locus_tag="VFH60_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6313757.1"
                     /translation="HLMTTSGSQPAIYTRPAELLQDLLRFDTTNPPGKERECVYYIHN
                     LLYEAGFDTEIVGLDDNRPNLVTRLKGRGDAPGLLMQGHVDVVTTYGQDWQHPPFGGD
                     LIDGYIWGRGAIDMKSGVAMMIAALLRAKAEGLQPPGDVVLAVLADEEAGSDYGAKYL
                     VEHHPYLFDGVKYAIGEAGGSTEYMAGRKFYPIQIGEKQMCWMRATLRGPGGHASLPT
                     RGGAVAKLGRMLTKLDNHRLPVHITPPVRQSIEAIAAALPPEMSGLAMGLLDPATTDA
                     VLDQMAGLGPEIREVARSLDAVLHNTANATVLRGGEKTNVIPSEIVVEFDGRLLPGYK
                     PEDMLSELGSLLGDEVEVEVVRFTAFEKPIDMGLYDTLAGIMREMDPEGVPVPYVLSG
                     VTDAVNFAKLDIQSYGFTPMKLPEGFNYIETVHAADERIPAEAMDFGTEAIYKLLTRF
                     DV"
BASE COUNT          374 a          741 c          756 g          458 t
ORIGIN      
        1 tggggttgag atggatgctg ctaggctgct agcttcagta taccagctag ccactccgtc
       61 gcctgagggt agtggtcgca gtccagcacg aactcctggc cccggcccac ggtggagagt
      121 aacgtgtggt ctgcttgcaa gctccagcga tttgcattgg tgtgatagct aagcctggga
      181 agcccctcct gggggtagaa ccaaccgggt agctgccagg tgctgcgcag gccgccgggg
      241 cacgtcaggc gcaggcggtc gtcggacatg gtaaagacgc ctcctccggg caggctgccg
      301 tccatgtcgg ggagttgcag gcggtcggtg gagacgtaaa gggagtcgag cctgctgtgc
      361 tgctcccgcg agcagtgcgg gtgatatagc gaccacgaag gtagctcgcg gcgattggcg
      421 accggtatgc ggcgctctac ctggagccaa ccgaagatgt agtggcagtc gggcgcatcc
      481 ttgacgtagc ggtaatgccc ctctgccagc tcggtctgcc tgaaccagcc gaagaagacg
      541 aagaggtcgc ccgcgctcac gccttgccgc tgcaaatggc tctccgccgc cccgtcctgc
      601 ccgaatatgg gcttccaacc tgcctccctg gggatgctgg agtagcgcag gtcggggtcc
      661 aggtgggctt cagcgtcagg tccaatcttc tcaagtttgc gcttgccgct tgtcaggtca
      721 ccaactagcg ttcccaggct aatatcgccc gccatgatgt cggcgtagcg aatgggccgg
      781 ttggactctc cgcctggtat cgggagcgag caaagttgcc cgttcgggag gatagggctt
      841 gccaccttgc ccgccgccga gtcgaagccc ttcctgctga ggatgatttt cacctggctt
      901 ttccatgttt ggcggcaggg cacggttgcc aaacctcgcg gtagtgccct gccgcatctc
      961 gttcgagcct aaacgtcgaa ccgggtgagc agcttgtata tcgcctcggt gccgaagtcc
     1021 atcgcctcgg ctgggatgcg ttcgtcggcg gcgtgcaccg tctcgatgta gttgaagccc
     1081 tccggcagct tcatgggggt gaagccgtag gattgtatgt ccagcttggc gaagttcacc
     1141 gcgtcagtca ccccgctcaa cacgtagggc accggcaccc cctccgggtc catctcgcgc
     1201 atgataccgg ccagcgtgtc gtacaggccc atatcgatag gcttctcgaa ggcggtgaag
     1261 cgcaccacct caacctccac ttcgtcaccc agcagcgacc caagctccga cagcatatct
     1321 tcgggtttgt agccgggcag cagccgcccg tcgaactcca ccacgatctc gctggggatc
     1381 acgttggtct tttcgccgcc gcgcaggacc gtggcgttgg ccgtgttgtg cagcaccgcg
     1441 tcgagcgacc gggccacttc cctaatttcc ggcccaagac ctgccatctg gtcgagcacc
     1501 gcgtcggtcg tcgccgggtc cagcaggccc atagcgaggc cgctcatttc gggcgggagg
     1561 gcggccgcta tcgcctctat cgactggcgc acgggcgggg taatgtgcac cggcaggcgg
     1621 tggttgtcga gtttggtgag catgcggccc agcttggcga cggccccgcc acgcgtgggg
     1681 agcgaggcat gcccgcccgg cccgcgcaac gtggcccgca tccagcacat ctgcttctcg
     1741 cctatctgta tggggtagaa cttgcggccc gccatgtact cggtgctgcc cccggcctcg
     1801 cctatcgcgt acttcacgcc gtcgaacagg tacgggtggt gctccacaag gtacttggcc
     1861 ccgtagtcgc tgcccgcctc ctcgtccgcc agtaccgcca gcaccacgtc gccgggcggt
     1921 tgcaagccct cggccttggc ccgcagcagc gcggcaatca tcatcgccac gcccgatttc
     1981 atgtcgatag cgccccggcc ccagatatag ccgtctatca ggtcgccccc gaagggtggg
     2041 tgctgccagt cctgcccgta ggtggtcacg acatccacgt gcccctgcat gaggaggccg
     2101 ggcgcatccc cacgcccctt caagcgggtc acgaggttgg ggcggttgtc atcgaggccc
     2161 acgatttcgg tgtcgaagcc cgcttcgtag agcaggttgt gtatgtagta gacgcactcg
     2221 cgctccttgc cgggagggtt ggtggtgtcg aagcggagga ggtcttgtaa gagttccgcc
     2281 ggacgggtgt atatggccgg ttggctgccg gaagtggtca taaggtgct
//
LOCUS       DASRWZ010000512         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1000833, whole genome shotgun sequence.
ACCESSION   DASRWZ010000512 DASRWZ010000000
VERSION     DASRWZ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000833"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..2757
                     /locus_tag="VFH60_07980"
     CDS             76..2757
                     /locus_tag="VFH60_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET6313758.1"
                     /translation="MSRKTFLESLSERVLVFDGAMGTSVQRYNLTPEEFGGPQLEGCN
                     DYLAVTNPRVIEEIHASFMEAGADVLETATFGSTRLKLDEYGIGEEVYHQNYTAAQLA
                     RKVADSFASPSRPRYVAGSMGPTGFLPSADDPTLSKVTYQQLKAVFKEQARPLVEGGV
                     DLLIIETAQDILELKAAINGVLEYFRESGRRVPIQAQVTLDTSGRMLLGTDIVAALTT
                     LSALHADIIGLNCSTGPEHMREPIRFLGQSATRWVSCIPNAGLPINVGGQAVYPLEAE
                     PMAQALYEFVNEFGVSVVGGCCGTGPEHIKALVEALGDRRKQHARPTYFEAHVSSGMR
                     ATPLAQVPAPLIVGERVNAQGSRKVKRLLLAEDYDAIVQVGREQVEGGAHVLDVQVAL
                     TERADEDAMMRALVKKLSMSVEVPLMIDSTEASAMQAALETYPGRAILNSINMERGRE
                     RIEAVLPLAYEHGAAVVALSIDEIGMAHTADRKVEICRRIYDIAMQEYGLTPDALIFD
                     VLTFPVTTGQEDLAPSALETLEGIRRVKVECPGSFTVLGVSNVSFGIDPYPRAVLNSV
                     FLHHAVQAGLDLAIVNPAHITPYAEINPEERRLADNLINNQPNALPEYIAYFEVNKRA
                     PEDKAAADDAFLHMNVAERIHYQILHRRKEGIEALLDEAMLDRTPVDVLNGILLPAMK
                     DVGDKFGAGELILPFVLQSAEVMKRAVAHLENFFEKKEGYTKGRVVLATVFGDVHDIG
                     KSLVNTILTNNGYTVYDLGKQVPLNVILDKAVEVDATAIGLSALLVSTSKQMPLCVQE
                     LHKRKLEYPVLVGGAAINRPFGYRIGFVEGDERYGPGVYYCRDAFEGLDTMEVLTDEE
                     KRQAFIDKRHNEARFIRTASACAWPTTSPRKTRAAWT"
BASE COUNT          520 a          813 c          989 g          467 t
ORIGIN      
        1 gaggactgag tgggtggtcc gaggaacctc agtcctcagt cctcagtcct catcactcgt
       61 tactggagca gtggcatgag cagaaagacc tttcttgaga gcttgagcga gcgggtgctc
      121 gtgttcgatg gcgctatggg cacgtcggtg cagcggtaca atttgacgcc ggaggagttc
      181 ggtggaccgc agcttgaggg ttgcaacgac tacctcgcgg tgacgaaccc gcgcgtgatc
      241 gaggagatcc atgcctcgtt catggaggcg ggggccgacg ttctggagac ggccacgttt
      301 ggcagcacgc gcctcaagct agacgagtac ggtatcggcg aggaggtcta ccaccagaac
      361 tacaccgccg ctcaactggc tcgcaaggtg gccgactctt ttgccagccc ctcgcggccg
      421 cgttacgtcg cggggtcgat ggggcctacc gggttcctgc cgtccgccga cgaccctacg
      481 ctctccaagg tgacgtacca gcagcttaag gcggtgttca aggagcaggc gcgcccgctg
      541 gtcgaaggcg gagtggacct gcttattatc gagacggcgc aggacatcct cgaactgaag
      601 gccgctatca acggcgtgct tgagtacttc cgcgagagcg gccgccgcgt gccgatacag
      661 gcgcaggtga cgctcgatac gtccgggcgc atgctcctgg gcaccgatat tgtggcggcg
      721 ctcactacgc tgtcggccct gcatgcggac atcatagggc tgaactgctc gaccgggccg
      781 gagcacatgc gcgagccgat tcgcttcctg ggtcagagcg ccacgcgctg ggtgtcgtgc
      841 atccccaacg cggggctgcc catcaatgtg ggcgggcagg cggtgtaccc gctcgaagcc
      901 gagcctatgg cgcaagcgtt gtacgagttc gtgaacgagt ttggcgtgag cgtggttggg
      961 ggctgttgcg gcacggggcc ggagcacatc aaggcgctgg tggaggcgct gggcgacagg
     1021 cgcaagcagc atgcccgccc gacctatttc gaggcccacg tttcgagcgg tatgcgggct
     1081 acgcccctgg cgcaggtacc tgcgcccctg atcgtggggg agcgggtgaa tgcgcagggg
     1141 tcgcgcaagg tgaagcgcct gctgctggcc gaggactacg acgccatcgt gcaggtgggc
     1201 cgcgagcagg tggagggcgg ggcgcacgtg ctcgacgtgc aggtggccct caccgagcgg
     1261 gcggacgaag acgcgatgat gcgcgcgctc gtgaagaagc tgagcatgag cgtggaagtg
     1321 cccctcatga tcgacagcac cgaggcgtca gcgatgcagg ccgcgttgga gacgtacccg
     1381 ggcagggcca tcctcaactc catcaacatg gagcgtgggc gggagaggat cgaggcggtg
     1441 ctgccgctgg cgtacgagca cggcgcggcg gtggtggccc tgtcgattga cgagataggc
     1501 atggcgcaca cggcggaccg caaggtcgag atttgcaggc gcatctacga catcgccatg
     1561 caggagtacg gcctcacgcc cgatgcgctg atattcgacg tactcacctt cccggtgacg
     1621 acggggcagg aggacctcgc gccctcggcc ctggagacgt tggagggcat ccggcgggtg
     1681 aaggtggagt gccccggctc gtttacggtg ctgggtgtga gcaacgtgtc gttcggcata
     1741 gacccttacc cgcgggcggt gctcaactcg gtctttctgc accacgccgt gcaggcgggg
     1801 ctggacctgg cgattgtgaa cccggcgcac atcaccccgt acgccgagat caacccggag
     1861 gagcggcggc tggccgacaa cctcatcaac aaccagccaa acgccctacc ggagtatatc
     1921 gcctactttg aggtcaacaa gcgcgccccc gaagacaagg cggcggcgga cgatgcgttc
     1981 ctgcacatga acgtggccga gcggattcac taccagatac tccaccgtag gaaagagggc
     2041 atcgaggcgc tgctggacga ggccatgctg gaccgcaccc cggtggacgt actgaacggg
     2101 atattgctcc cggcgatgaa ggacgtgggc gataagtttg gcgcgggcga gctgatactg
     2161 ccgttcgtgc tgcaatcggc ggaggtcatg aagcgggcgg tggcccacct ggaaaacttc
     2221 ttcgagaaga aggaaggcta cacgaagggc cgcgtggtgc tggcgacggt gtttggcgac
     2281 gtgcacgaca tcggcaagag cctggtcaat accatcctca cgaataacgg ctatacggtg
     2341 tacgacctgg gcaagcaggt gccgctgaac gtcatactcg ataaggcggt ggaggtggac
     2401 gcgacggcta tcggactcag cgcgctgctg gtgtccacct cgaagcagat gccgctctgc
     2461 gtgcaggagc tacacaagcg caagctggaa tacccggtgc tggtgggtgg cgcggccatc
     2521 aaccggccct ttggctaccg catcggcttc gtggagggcg acgagcgtta cgggccgggg
     2581 gtgtactact gccgcgatgc gttcgagggg ctggatacga tggaggtgct gacggacgag
     2641 gagaagcggc aggctttcat cgacaagcgg cacaacgagg cccgcttcat acggaccgcg
     2701 agcgcctgtg cctggccgac tacttcgcct cgaaagactc gggccgcatg gacgtgatac
     2761 cgctacaggt ggtcacgatg ggcgaccgg
//
LOCUS       DASRWZ010000513         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_590336, whole genome shotgun sequence.
ACCESSION   DASRWZ010000513 DASRWZ010000000
VERSION     DASRWZ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590336"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFH60_07985"
     CDS             <1..249
                     /locus_tag="VFH60_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313759.1"
                     /translation="SGGGGSMGGSGGGSGSGSGGGTWESGGGSGSGGSGGGGSMGGSG
                     GGSSSGGGGGMSGDSGGGSGSGGSSGGGGSSSGGGGGG"
     gene            611..>3495
                     /locus_tag="VFH60_07990"
     CDS             611..>3495
                     /locus_tag="VFH60_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6313760.1"
                     /translation="MAPEVGGRRGIRLVVAGALCMLVGLLVWLGKGHTAGQVNGPGLP
                     PNRPMAPQAGQVGQAGHAAQPGTSPSQQDLISPGRLIEGRPARHDTSPPLRDMQPALS
                     TPAPAQRENNEARLPFDPGSTKPVNDPVAQRFLGPLAMPSVITQFAGIGASMSFCNCV
                     PPDPVGDVGPNHYVQMVNKAFQVFNKSGTSLMGPLPINTLFDNFGGVCESTNSGDPIV
                     LYDPLADRWLLSQFVFPSPHGQCIAVSATGDPTGAYHRYYYQFDDPSILYDYPKFGVW
                     PDGYYMTANRYNNVDAYQGPAVMVFERDKMLVGDEARKLEYNPGNYFVSLLPADLDGA
                     TLPPAGAPNYLASTSLSLNQSSIYLWEFHTDWQNTGMTTLTGPATVPVAAFDPNLCNG
                     SGNCIPQPGTTLGIDPINGQLMFRLAYRNFGTHESLVSAQTVDENGADHAGIRWYEVR
                     DPGGSPFVYQQGTYAPDSDHRFMPSMAMDRDGNIAIGYSVSSPNVFPSIRYTGRMASD
                     PPGVLGLGEATLYAGGGSQTGSTRWGDYSAMSVDPSDDCTFWYTNEYYSSSSSTGWKT
                     RIGSFKLPGCGQTTPTPTVTGTPPTATPSIIPSATPSQVPASCANYTVQTSTGGVIDT
                     TGKALIPNTQCDDCTTYITLPFAVDLYHETYTNAYVSSNGVLQFGSSDFSFGNDCLPV
                     PVFDHAIAAYWDDLYTDGEGLGVYWKVSGQAPNRKYSLEWRAGTYSSPDTDPVNFEVR
                     LREGSSNFEIVYSQVYSNGHDATVGVQSSYQGASTAYTLVSCYSSTLTPGTVLNFTLQ
                     PCPTCTLQFQDVAQDNTFYPFVRCLACKNILGGYPCGGPGEPCNGSGDPYFRPANNVT
                     RGQLSKIVSESAGFNNSIPASRRTYEDVPPDSPFWLWIERLTVKGVMSGYPCGGDMEP
                     CIVPDSTYFRPQGYATRGQLTKIVANAAAFSDTPPQGEYTFADVLPD"
BASE COUNT          634 a         1260 c         1013 g          588 t
ORIGIN      
        1 tccggtggcg gcggctcgat gggcggctcc ggcggcggca gcggtagcgg ctccggcggc
       61 ggcacttggg aaagcggcgg cggctccggc agcggcggct ccggtggcgg cggctcgatg
      121 ggcggctccg gcggtggtag cagctccggc ggtggcggcg gcatgagtgg cgactccggt
      181 ggcggctctg gcagcggcgg ctcctccggt gggggcggta gctcctccgg tggcggtggc
      241 ggcggctgaa attagcctaa ccacaggcat acgtaattgt cgcactagct ggccctatac
      301 ggcccttcgt cacactggcc cctccatatc cttcccggcc ccagtgtgag ccaatgccaa
      361 tatagtggct ccttgataca aaagggacgt accgaccccc ggtacgtccc ttttgtattg
      421 ggcctgcgtg cgaacatgcc tccgtgtgtc tccagcgctc atcgcgcctc agacatcgcg
      481 cctcagactc tgctcccgca cgcaggcatg tgcaggtgcg ggcacttatt cggtatactt
      541 gacctgtaat ctcgcgtttt agcatgcagc ccttagttgg gccgggccgt tcttgcgttg
      601 ggagaggtac ttggcacctg aggtcggagg cagacgaggt attcggttgg ttgtggcggg
      661 cgcgctctgc atgctcgttg gcttgctcgt gtggctcggc aaaggtcata cagccggaca
      721 ggtaaacggg ccgggcttgc cgcccaaccg ccccatggct ccccaggcag ggcaggtcgg
      781 gcaggccggc cacgcggcac aacccggcac aagcccctca cagcaagacc tcataagccc
      841 aggacgtctc atcgaggggc ggcccgccag gcatgatacc tcgccgcccc tgcgcgacat
      901 gcaacccgcc ctgtctacac ccgcaccggc ccagcgcgag aacaacgagg cccgcttgcc
      961 cttcgacccc ggttccacaa agccggtaaa cgaccccgtc gcccagcgct tcctcggccc
     1021 tctcgccatg ccgtccgtca tcacccagtt cgcgggtata ggggccagca tgtccttctg
     1081 caactgcgtc ccgcccgacc cggtgggcga cgttggcccc aaccactacg tgcagatggt
     1141 caataaggcc ttccaggtct tcaataaaag cggcacctcc ctcatgggac ctctgcctat
     1201 caacacgcta tttgataact tcggtggggt ctgcgaaagc accaacagcg gcgacccaat
     1261 cgtgctgtac gaccccctgg cggacaggtg gctgctcagc cagttcgtct tcccctcgcc
     1321 gcacggccag tgcatcgctg tctccgccac gggcgacccc acaggcgctt accaccgcta
     1381 ttactaccag ttcgatgacc ccagcatcct gtacgactat cccaagttcg gcgtgtggcc
     1441 cgacggctac tacatgaccg ccaaccgcta caacaacgtc gatgcctacc agggtccggc
     1501 tgtgatggtg ttcgagcgcg acaagatgct ggtgggcgat gaggcccgca agctggagta
     1561 caaccccggc aactactttg tgtcgctact accggccgac ctcgatggag ctacgctgcc
     1621 gcccgccggc gcgcctaact acctcgcctc tacctcgtta tctctcaacc agagcagcat
     1681 atacctgtgg gagttccaca ccgactggca gaacaccggc atgaccacgc tgaccggccc
     1741 cgctaccgta cccgtggccg cgtttgaccc caacctgtgc aacggctccg gcaactgcat
     1801 cccccagccc ggtaccacgc tcggcataga cccgataaac ggccagctca tgttcaggct
     1861 cgcttaccgc aatttcggca cgcacgagtc gctcgtcagc gcgcagacgg tggacgaaaa
     1921 cggggccgac cacgcgggca tccggtggta cgaggtgcgc gaccccggcg gcagcccctt
     1981 cgtctaccag cagggcacct acgcgcccga ttccgaccac cgcttcatgc ccagcatggc
     2041 gatggaccgc gacggcaaca tcgcgatagg ctacagcgtc tcaagcccca acgtctttcc
     2101 ctctatccgc tacacgggcc gcatggcaag cgatccgccc ggggtgctgg gcctgggtga
     2161 ggccaccctg tacgccggtg gaggctcgca gaccggcagc acccgctggg gcgactactc
     2221 ggccatgtcg gtcgatccaa gcgacgattg caccttctgg tacaccaacg agtactattc
     2281 gagcagttcc tccaccggtt ggaagacgcg catcggcagc ttcaagctcc ccggctgcgg
     2341 ccagaccacc cccacgccca cggtgaccgg cacccctccc accgccaccc ccagcatcat
     2401 acccagcgcg actccctccc aggtgcccgc ctcctgcgct aactacacgg tgcagacctc
     2461 caccgggggc gtgattgaca ccacaggcaa ggccctcata cccaataccc agtgcgacga
     2521 ctgcaccact tacatcaccc tgcccttcgc ggtagacctg taccacgaga cctacaccaa
     2581 cgcctacgtt agctccaacg gcgtgctcca gttcgggagc agcgacttct ccttcggcaa
     2641 cgactgcctg cccgtgccgg tgttcgacca cgccatagcc gcctactggg acgacctcta
     2701 cacggacggc gagggcctgg gcgtctactg gaaggtgagc gggcaggccc ccaaccgcaa
     2761 gtacagcctc gaatggcggg ccgggaccta cagcagcccc gatacggacc cggtcaactt
     2821 cgaggtccgc ctgcgcgagg gcagcagcaa cttcgagatt gtctattcgc aggtctacag
     2881 caacgggcat gacgccacgg tgggcgtgca aagcagctac cagggtgcga gtacggccta
     2941 caccctcgtg tcgtgctaca gttccaccct cactcccggc acggtgctga acttcacact
     3001 ccagccttgt ccgacctgca cgttgcagtt ccaggacgtg gcgcaagaca ataccttcta
     3061 ccccttcgta cgctgcctgg cctgcaagaa catcctgggc ggctatccct gcggcggccc
     3121 cggcgagcca tgcaacggca gcggcgaccc ctacttccgg cccgccaaca acgtcacgcg
     3181 cggccagcta tcgaagatag tgtcggagag cgcgggcttc aataacagca tcccggcctc
     3241 ccgccgcacc tacgaggacg tgccgcctga cagccccttc tggctctgga tagagaggct
     3301 caccgtcaag ggcgtaatga gcggctaccc atgcggcggc gacatggagc cttgcatcgt
     3361 gccggacagc acctacttcc gcccgcaggg ttacgccaca cggggccagc tcaccaagat
     3421 agtcgccaac gccgccgctt tcagcgacac gccgccccag ggcgagtaca cctttgccga
     3481 cgtgctcccc gacag
//
LOCUS       DASRWZ010000514         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1343542, whole genome shotgun sequence.
ACCESSION   DASRWZ010000514 DASRWZ010000000
VERSION     DASRWZ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343542"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..480)
                     /gene="moaC"
                     /locus_tag="VFH60_07995"
     CDS             complement(7..480)
                     /gene="moaC"
                     /locus_tag="VFH60_07995"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET6313761.1"
                     /translation="MSELTHLDESGHARMVDVGQKPDTERIAIARAEVRISQGTLDLV
                     REGKAAKGDVLAVARVAGIMAAKKTSELIPLCHPIMLTSVKVDFAFREWGIEIEARTK
                     TTGPTGVEMEALTAASVTALTIYDMLKAVERGAEITNIRLIHKEGGKSGMWERET"
     gene            complement(487..>1501)
                     /locus_tag="VFH60_08000"
     CDS             complement(487..>1501)
                     /locus_tag="VFH60_08000"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HET6313762.1"
                     /translation="SVLVGQLRKAGVDRARVLVVGAGPTAAVVVDRLAHHPTLGYEVL
                     GMVDHNTNGNADKRGAGHVPVLGNITRLAEIVRQRNVDEVIVALSGASDKQLRDIITL
                     VQDESVSVKIYPDAFQLMTQNEVSVGELSGLPLLSVKDVALRGWNRRLKRAFDVVFSL
                     IVLVLTAPIMLVIALAIKLTSHGPVFFIQERVGLDGTPFQLVKFRTMRIAVEEGTPGW
                     TVPNDPRRTSIGTFLRRFSLDELPQFYNVLMGEMSVVGPRPEQPEYVKEFAQRIPHYL
                     RRHREKSGITGWAQVNGLRGDSSIELRTAADIYYVENWSLLFDLKIIVRTIVAMFRGK
                     NAY"
BASE COUNT          261 a          498 c          437 g          305 t
ORIGIN      
        1 tacgcatcac gtttcacgct cccacatgcc actcttgccg ccctctttat gtatcaggcg
       61 gatgttggtg atttccgcgc cacgctccac tgccttgagc atgtcgtaga tggtgagggc
      121 ggtgaccgag gcggcggtca gcgcctccat ttctaccccc gtagggccgg tggtcttcgt
      181 gcgggcttct atctcgatac cccactcacg gaaggcgaaa tccaccttta ccgacgtgag
      241 catgatgggg tggcaaagcg gaatgagttc ggaggtcttc ttagcggcca tgatgcccgc
      301 taccctggct accgcgagca cgtccccctt ggccgcctta ccttcgcgca caaggtcgag
      361 cgtgccttgg ctgatgcgga cctcagcgcg cgctatagct atgcgctcgg tgtccggctt
      421 ctgcccaacg tccaccatgc gggcgtggcc gctctcgtcc aggtgagtca gttctgacat
      481 agcggcctaa taagcgttct tgcccctgaa catcgccact atcgtgcgca cgatgatctt
      541 gaggtcgaag agaagggacc agttctccac gtaataaatg tcggctgccg tgcgcaattc
      601 gatggagctg tcgccgcgca ggccgttcac ctgcgcccag ccggttatgc ccgacttctc
      661 gcggtggcgg cgcaggtagt gtgggatgcg ctgcgcgaac tccttgacgt actccggctg
      721 ctccggtcgc ggcccaacca cgctcatctc acccatgagc acgttgtaga actgcggcaa
      781 ctcgtccagc gagaagcgcc gcaggaaggt gccgatgctg gtgcggcgcg ggtcgttggg
      841 caccgtccac cccggcgtac cttcttccac cgctattctc atggtgcgga acttgaccaa
      901 ctggaagggc gtgccgtcca accccacccg ctcctgtatg aagaacacgg ggccgtgact
      961 ggtcagcttg attgccaggg ctattacaag catgatggga gcggtcagca cgagcactat
     1021 caggctgaac actacatcga acgcccgctt gagcctgcgg ttccagccgc gcaacgccac
     1081 gtccttgacg ctcagcaagg gcaggccgct caactcgccc acgctcactt cgttctgggt
     1141 catcagttgg aaagcatcgg ggtaaatctt gaccgatacg ctctcgtcct gcacgagcgt
     1201 tataatgtcg cgcaactgct tgtccgacgc gccgctcagg gcgactatca cctcgtctac
     1261 gttgcgctgg cgcacgatct ccgccaatcg cgtgatattg cccaggacag ggacgtgccc
     1321 ggcgccacgc ttgtcggcgt tgccgttggt gttgtggtca accatgccca gcacttcata
     1381 gccaagcgtg gggtggtgcg ccaggcggtc taccacgacg gcggctgtgg gccccgcgcc
     1441 cacgaccagc acccgcgccc ggtccacacc cgccttccgc aattgcccca ccaaaacgct
     1501 g
//
LOCUS       DASRWZ010000515         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1343611, whole genome shotgun sequence.
ACCESSION   DASRWZ010000515 DASRWZ010000000
VERSION     DASRWZ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343611"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..501
                     /locus_tag="VFH60_08005"
                     /pseudo
     CDS             310..501
                     /locus_tag="VFH60_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
     gene            520..1455
                     /locus_tag="VFH60_08010"
     CDS             520..1455
                     /locus_tag="VFH60_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001983651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313763.1"
                     /translation="MVSLTRRDGRPIPTPTPRPTATGACTVFPANNIWNRRVDTLPVH
                     PMSSAYIASIGSDANVHADFGSGLWDGGPIGIPFVKVSANQAMVDINFTAYGDESDPG
                     PYPVPTNAPVEGGPDADGDRHVLVLQESTCMLYELYRAFPNSDGSWDADSAAAYDLRS
                     NALRPEGWTSADAAGLPIYPGLVTYDEVASGAIRHAIRFTAPDTQRKYIWPARHYASD
                     SDDPDLPPMGLRVRLKSSVDLGHYPQQVRVILQAMKDYGLILADNGSPWYISGAPDPR
                     WDNDMLHAMDDLQGSDFEAVYTDDLMVDVNSGQSR"
     gene            1929..>2117
                     /locus_tag="VFH60_08015"
     CDS             1929..>2117
                     /locus_tag="VFH60_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313764.1"
                     /translation="MSNNDPNKPGWRFDPETGQPINPGPPPQPQTQPGWSFDPQTGQP
                     IPPPAPQPEQPSYTQPQPQ"
BASE COUNT          388 a          704 c          655 g          370 t
ORIGIN      
        1 atggatttga cgagcgtggg ggcggtatcg gggcgcgggg acggcacctt ccttcccctg
       61 tacgagatat cgcgcgggca ggtgatgaaa gtggtcgtgc aggcgttggg cttgagcgcg
      121 gctattccct cgtcgcctac cttcgccgac gtacccgcca gccacacctt ctaccagtgg
      181 atcgaggtag gcgcggctaa gggcctggcg agcggctacc cgtgcggtgg acccggggag
      241 gcttgcgatt ctcagcgcag gccctacttc cgctccaatt acagcgtaag ccgcggtcag
      301 cttgccaaga tgctggtgac ggcgaagaac tggacgccgc tcgcaccctc gcaggcgacg
      361 ttcagggacg taccggccgg cagcaccttc tacggctacg tggagcgggt ggcggctaac
      421 ggtatcattg gcggctaccc gtgcggtggg cgaggggaag cttgcccagg cacctacttc
      481 cggccctccg gcaccgctac ccgcgcccag gcaagcaaga tggtcagcct cacacggcgg
      541 gacggccgcc ccatacctac gcccactccc cgccccaccg cgaccggtgc gtgcactgtc
      601 ttcccggcca acaacatctg gaaccgcagg gtcgatacgc tgcccgttca tccgatgtcg
      661 agcgcctaca tcgcatcgat agggtcggac gcgaacgtgc acgccgactt cggctccggg
      721 ctgtgggatg gtggacctat aggcataccg ttcgtgaagg tgtccgcgaa ccaggcgatg
      781 gtggacatca acttcacggc gtatggcgac gagagcgacc ccggccccta cccggtgccc
      841 actaacgccc cggtggaagg cggccccgat gccgacggcg acaggcacgt gctggtgttg
      901 caggaaagca cctgcatgct gtacgagcta taccgcgcct tcccgaactc cgacggctct
      961 tgggacgccg acagcgccgc cgcgtacgac ctgcgctcca acgccctgcg cccggagggc
     1021 tggacctccg ccgacgcggc aggactgcct atctaccccg gcctggtgac ttacgacgag
     1081 gtggcttccg gggccatcag gcacgcgatt cgcttcaccg cgcccgatac ccagcgtaag
     1141 tacatctggc ccgcccgcca ctacgccagc gactcggacg atcccgacct gccgccgatg
     1201 ggcctgcgcg tgcgcctcaa gtcgagcgtg gacctgggcc actacccgca gcaggtgagg
     1261 gtgattctgc aggcgatgaa ggactacggc ctcatcctgg ccgacaacgg cagcccctgg
     1321 tacatcagcg gcgcgcccga cccccgctgg gacaacgaca tgctccacgc gatggacgac
     1381 ctgcaaggct ccgacttcga ggcggtgtat accgacgacc tgatggtgga cgtgaattcg
     1441 gggcagagta ggtgaggaga gttgttagtt gttagttgtt agttattagt ggcgagggtg
     1501 ccttgccatc gtcgccctgt gggggtgccc tgtggctgaa gccacgggct tacaaggggc
     1561 aaagtcccct acggggactg aggaacatgt agtagtctcc gtaggagact tcgtctctgg
     1621 tcagcccgcc aattcattgg cagggcaatg ccacaaggcg acgatggcag tgcgtctccc
     1681 gcacgctcca ctcgtcacta acaactaaca actaacaact aacaactctc ccatcgcttg
     1741 actaacaact gctttgcgca tacaatggcc gcgtaacttg ccgtttggca cgcttgattt
     1801 gggccttcca ccgctatgac gtgcggggga gtttggtatc ctgcgccggt atgcaggcgc
     1861 gggtgtgcgt tcttgaggct tcttctacct catggtagaa cgtgttttcc catataggag
     1921 gaagttctat gagtaacaac gaccctaaca agcccggttg gcgcttcgac ccggagacgg
     1981 gccagcccat caatcccggc cccccgcccc agccgcagac gcaacccggt tggagcttcg
     2041 acccgcagac cgggcagcca atccccccgc ccgcaccgca gccggagcag ccttcgtaca
     2101 cgcagcccca accccag
//
LOCUS       DASRWZ010000516         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_590550, whole genome shotgun sequence.
ACCESSION   DASRWZ010000516 DASRWZ010000000
VERSION     DASRWZ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590550"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2372
                     /locus_tag="VFH60_08020"
     CDS             <1..2372
                     /locus_tag="VFH60_08020"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6313765.1"
                     /translation="CPNCGSPIDVAAAVSPPAGGTTATSAIVCPVCSTTNPPGTEFCS
                     NCGAPLAQVLSTPLASGGDGLTTGITCGVCGHDIPPGEDYCPDCGAPRSASVQLQAPG
                     STGTAEAPPGPTTCPNCGAKLRPGARFCRECGTRFDSIATDQKSQGGLSQLKPGQVLA
                     GRYKIQSVIGGGGMGAVFKGVDMNLTSRAEPEGRQVAVKAILDTTDPELLAAAVEERE
                     MLVRLDHPNIVRIYDIVNEGGVPYIVMAYVKGKGWKQLYEENGGPLPESEALRLLLGM
                     RGAFEYLHHRVPPVVYRDFKPGQVIEVTDPNGEKRQVLIDLGTAMEYFPGQRRQAWGT
                     VGFAPPEIGGIVEQPPTMDLFGIVSTLAALLGVAVDRQPILAPPRHEWPVSEEIYDLV
                     MRGRDPDPRVRFQTVEELFNQLEGINRFIQGQERARVVGSPQVGGRGTRTLSQVLVPV
                     QSTLITGKLLNQQVTGKISALPVLSGADPAQQLLQPARDLVDAGRYVEALGQLDVVLR
                     QHPASIDGHLLRATALNNLGRTDEARAALDQANRLGTPATRWRTLLVQAQASETQGDY
                     RTAETMYQELIKLVPGEVLPKQALADLYSETQRYGEAADLYSRVVAADPANADAVLGW
                     ADSLVALNRTDVAIGVLDAVNENALRYVDAQLRLVELYLSRIDAYLTSRPGGLDGAKL
                     SNPDVQGMLRDLDLAGRAIAALNDRTESRHFYRLQADWWYAAYRLSRAGLLPQDISWP
                     DSRTDHSNKPDVVGHDCRQAYRRYLQRAPDAPDREEVLARIYFDVREWQ"
     gene            2445..3752
                     /locus_tag="VFH60_08025"
     CDS             2445..3752
                     /locus_tag="VFH60_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET6313766.1"
                     /translation="MVRFRVVPHPSEAQVLQGQNLARTMYSIGADAGGGGIGRQNVPV
                     FLGVVMDQSGSMEGSKLESAKEALIRLLHEVPDNDSVVVHITLFSDYAEELMPARTGR
                     EIEREMGSLTSRIRGINAGGQTSLGSGLRVALEASRRYPEYERRVLMITDGKQEGPVP
                     ITDAYQAADELARARIRIDAWGVGNAWEADELRTIAHTTGGEAEVVPNARDLAQAVTD
                     LFTDVRSTVASNVRLVLNTPVGTRIRSVRQVYPSVQDRAAEQVNEQKWVISVGSLGNE
                     EPKFVIELETTPRGNDIPFRILVPTVLYNLGTEEQVDELDRSSWFFVRWVGSPGQIKM
                     DEQLTRFTGEEEIAALSKEGFALLDMGAVDEATAKLSAALQRATQINSPQAVVLSAVI
                     NPRTGKLVSTNASDAVNKTGKLHTGKTGKIMAGTGKLPTPQTG"
     gene            3926..4645
                     /locus_tag="VFH60_08030"
     CDS             3926..4645
                     /locus_tag="VFH60_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313767.1"
                     /translation="MILLVTLLIALAGQTWIWFFGALLVLVVLYPSLFNIGAGRAAGT
                     SAAPTTTAPGPDLNQLQGRYREQVQRALTARQNIEAAISRTQDQGQRKVLMDAVGALP
                     EVTNSIYNLALKAQSVDTALGGANPIDRLTGEIKQLEAQIHSTNDEFQKSQYYAALDG
                     KLQQMQNLTDTTVAMRRWDSQIENALSTLDTLLSQVLRIKSSEVLSYNGATDELSNSL
                     KREVESLKATAEAMDNVYGWK"
BASE COUNT          934 a         1646 c         1486 g          744 t
ORIGIN      
        1 attgccccaa ctgcggctcc ccaatcgacg tagcagccgc agtctcccca cccgcgggcg
       61 gcaccacagc cacgtccgcg atagtctgcc ccgtctgtag caccaccaac ccgcccggca
      121 ccgaattttg ctccaactgc ggcgcgcccc tggcacaggt gctcagcacg cccctggctt
      181 caggtggcga tggcctcact accggcatca cgtgcggcgt ctgcggccac gacattcccc
      241 ccggtgagga ctactgcccc gactgcggcg cgccccgtag cgcatcggtc cagctacaag
      301 cccccggttc gaccggtacc gccgaagcac ctcctgggcc aacaacgtgc cccaactgcg
      361 gcgcgaagct gcgtcctggg gccaggttct gccgggagtg cggcacccgc ttcgacagca
      421 ttgccacgga ccagaagagc cagggcggcc tcagccagct aaagccgggc caggtgctcg
      481 ctgggcgcta caagatacag tccgtgattg gtggcggggg tatgggcgcc gtgttcaagg
      541 gcgtcgatat gaacctcacc tcccgcgccg agccggaggg ccgccaggtc gctgtgaagg
      601 ccatactcga caccaccgac cccgagctgc tcgccgccgc cgtggaagag cgcgaaatgc
      661 tcgtgcgcct cgaccacccc aacatcgtgc gcatttacga catcgtcaac gagggcggcg
      721 taccctacat cgttatggcc tacgtcaagg gcaagggctg gaagcagctt tacgaggaga
      781 acggcggccc gctccccgaa agcgaggcgt tacgcctgct gctcggcatg cggggcgcgt
      841 tcgagtacct tcaccatcgc gtgccccccg tcgtgtaccg cgacttcaag ccgggccagg
      901 tcatcgaagt gaccgacccc aacggcgaga agcgccaggt gctgatagac cttggcaccg
      961 cgatggagta cttccccggc cagagaaggc aggcctgggg cacggtgggc ttcgcgcccc
     1021 ccgaaatcgg cggcatcgtc gagcagcccc ccacgatgga cctgttcggc atcgtgagca
     1081 cccttgctgc cctgctgggc gtggccgttg acaggcagcc gatactcgcc ccgccccgcc
     1141 acgaatggcc cgtgtcggag gagatttacg acctcgtcat gcgcggccgc gaccccgacc
     1201 cccgcgtgcg cttccagacc gtcgaggagc tgttcaacca gcttgagggc atcaaccgct
     1261 tcatccaggg ccaggagcgg gccagggtgg tcggatctcc gcaggtaggc ggacggggca
     1321 cgcgcaccct ttcgcaggtg ctggtgcccg tgcaaagtac tctcatcacc ggcaagctgc
     1381 tcaaccagca ggtcaccggc aaaatcagcg cgctacccgt gctcagcggc gcggaccccg
     1441 ctcagcaact gctccagccc gcccgcgacc tggtagacgc cgggcgctac gtcgaagcgt
     1501 tgggccaact cgacgtggta ttgcgccagc accccgccag catcgacggg cacctgctca
     1561 gggccaccgc cctcaacaac ctgggccgca ccgatgaggc cagggccgcg ctcgaccagg
     1621 ccaaccggct gggcaccccc gctacccgct ggcgcacgct cctggtgcag gcgcaggcat
     1681 ccgagacgca gggcgattac cgcacggccg agacgatgta ccaggagctt atcaagctgg
     1741 tacccggcga ggtgctgccc aagcaagccc tggccgacct ctacagcgag acccagcgct
     1801 acggcgaagc cgccgacctc tacagccgcg tggtggccgc cgaccccgcc aacgccgatg
     1861 ccgtgctggg ctgggccgat agcctggtgg cactgaacag gaccgacgtg gctataggcg
     1921 tgctcgatgc ggtgaacgag aacgccctgc gctacgtgga cgcccagctt cgcctggtag
     1981 agttgtacct ctcgcgcatc gacgcttact tgacctccag gcccggcggg ctcgacggcg
     2041 cgaaactgag caacccggat gtgcaaggca tgctccgcga cctcgacctt gcagggcgcg
     2101 ccatcgccgc cctcaacgac cgcaccgagt cgcgccactt ctaccgcctg caagccgact
     2161 ggtggtacgc cgcgtatcgc ctctcaagag caggactgct cccgcaggac atcagctggc
     2221 ccgacagccg caccgaccac agcaacaaac ccgacgttgt gggccacgac tgccgccagg
     2281 cctaccgccg ctatctacag cgcgcccctg atgccccgga cagggaggag gtcctggccc
     2341 gcatctactt tgacgttcgg gaatggcagt agggattttg gattttaggt tttggatttt
     2401 ggattggacg tccaatctag aacccaggat tgaaggaggc ttttgtggtt agatttcgcg
     2461 tagtccctca cccctctgag gcgcaggtgc tgcaaggaca gaacctggct cgaactatgt
     2521 acagcattgg cgctgacgcg ggcggcggtg gaattggtcg gcagaacgtg ccggtgttcc
     2581 tgggtgtcgt catggaccag tctgggagca tggaaggcag caagctcgag tcggccaaag
     2641 aggccctcat caggctgttg cacgaggtgc ccgacaacga cagcgtcgtg gtccatatca
     2701 ccctgttcag cgactacgcc gaggagctaa tgcctgccag gacgggccgc gagatcgagc
     2761 gcgagatggg cagcctcacc tcgcgcatac ggggcatcaa cgcgggcggc cagacttccc
     2821 tcggctccgg cctgcgggtg gcgcttgaag cttcgaggcg ctaccccgaa tatgagcgcc
     2881 gcgtgctgat gatcaccgat ggcaagcagg aggggccggt gcccatcacc gatgcctacc
     2941 aggccgccga cgagcttgcc cgcgcccgca tccgcatcga cgcctggggc gtgggtaatg
     3001 cctgggaggc cgacgaactg cgcaccatag cccacaccac cggcggcgag gccgaggtgg
     3061 tgcccaacgc ccgcgacctc gcgcaggccg tgaccgacct gttcaccgac gtgcgcagca
     3121 ccgtggcgag caacgtgcgc ctggtgctca acactcccgt cggcacccgc atacgaagcg
     3181 tgcggcaggt ctacccctcc gtgcaggacc gcgccgccga gcaggtcaac gagcagaagt
     3241 gggtcatctc ggtcggctcc ctgggcaacg aagaacccaa gttcgtgata gagctggaga
     3301 ccaccccgcg cggcaacgac atcccgttcc gcatcctggt gcccaccgtg ctctacaacc
     3361 tcggcaccga ggagcaggtg gacgaacttg accgctcgtc ctggttcttc gtgcgctggg
     3421 tcggctcgcc cggacagatc aagatggacg agcaattgac ccgcttcaca ggcgaggagg
     3481 agatagccgc cctctccaag gaaggcttcg ccctgctcga catgggggcc gtcgatgagg
     3541 ccaccgccaa gctcagcgcc gccctccaac gcgccacgca aatcaacagc ccccaggccg
     3601 tcgtgctcag cgccgtaatc aacccgcgca ccggcaagct cgtctccacc aacgccagcg
     3661 acgcggtaaa caagaccggc aagctgcaca ccggcaagac aggcaagata atggcgggca
     3721 cgggcaagct gcccacgccc cagacgggct gagtgacgag tgacgcgtaa ccctgaagca
     3781 aattctggcg taattattac gtatcactcg tcactcgtca ctcgtcacgc acgaaaggaa
     3841 cttgatgaac aacggcgcta atctagggga tatgatcagg ggattttggc agagggtgca
     3901 ggcccatccc gctcaggcgg cttttgtgat actgctcgtt accctactca tcgcgttggc
     3961 ggggcagacc tggatatggt tctttggggc cttgctggtg ctggtggtgc tttacccgtc
     4021 gctgttcaac atcggggcgg ggcgcgcggc gggcacgagt gccgcgccaa caaccaccgc
     4081 gccagggccg gacctcaacc agttgcaggg ccgctaccgc gagcaggtgc agcgcgccct
     4141 caccgcccgc cagaacatcg aggcagccat aagccgcacc caggaccagg gccagcgcaa
     4201 ggtgctcatg gacgcggtcg gcgcgctgcc ggaggttacg aacagcattt acaacctggc
     4261 gctcaaggcg caaagcgttg atacggccct cggcggcgct aacccgatag accgcctgac
     4321 cggcgagata aagcagttgg aggcgcagat ccactccacc aacgacgagt tccagaagag
     4381 ccagtactac gcggccctcg acggcaagct gcaacagatg cagaacctca ccgacaccac
     4441 ggtagccatg cgccgctggg actcgcagat cgagaacgcc ctcagcaccc tcgacaccct
     4501 gctctcgcag gtgttgcgca tcaagtcgtc cgaagtgctc tcctacaacg gcgcgaccga
     4561 cgagctatcc aacagcctca agcgggaggt cgaatccctc aaggccaccg ccgaggctat
     4621 ggacaacgtc tatgggtgga agtaaggata gcattgccga cccgtgaaca tgaccatatt
     4681 cattagtcgg gaccatattc ccccttgtca tcctgagcgt agcgaaggat caggtgggta
     4741 agcgagagtc tacaccctgc aggatgttgt cggctagagg aacattgttc cccaaattgt
     4801 cggggtcagg
//
LOCUS       DASRWZ010000517         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_795962, whole genome shotgun sequence.
ACCESSION   DASRWZ010000517 DASRWZ010000000
VERSION     DASRWZ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795962"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFH60_08035"
     CDS             complement(<1..1135)
                     /locus_tag="VFH60_08035"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HET6313768.1"
                     /translation="MQTILPAIYVLGHKNPDSDAICSAVGYAALLHAQGRTNAVAARQ
                     GEMRPETAYILERFGVPAPLFVDDVRPRVVDVMTRSVTTVHAETSLYEVGQIMQQGGV
                     RAVPVVDDEGHLCGITGIEDFARSFISGLDSDQLDHVHLNLDNVLSALGGKLLVAAPG
                     RTLRDQVMVGAMEIDSMLKRIAPDILLVMGDRIDAQRAAIEFGVGALVLTGGTPVSEE
                     ILDLARERQVTVISVEHHTYTTVRLIQMSAAVRHVMRKEVVTCEPDDLVEEVRETLQA
                     GRVRALVVTDGDKKVLGIISRTNLLRQVRRQVVLVDHNERSQSVAGIEEADVIGVIDH
                     HRVADFQTRSPAFMRLEPVGATSTIVGKLFSEANIPVPQPIAGV"
     gene            1479..>2169
                     /locus_tag="VFH60_08040"
     CDS             1479..>2169
                     /locus_tag="VFH60_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313769.1"
                     /translation="MPKNRGRGTGSEGRGARIGGQGSGSAAHARVDDVAGQDAQLRGV
                     PGRPRHGRQGGSFAARFGRLILQHGIAAIPSALYHYQGEMQLSAQQVWLVSYILSHKW
                     DEDLPYPSLKKMARCTTLSLSQLQRIKNSLCDMGYLKVYPRFDEKQGQNTNAYDFSGL
                     FDRLEELITGDAPVANEIRAESEGPDLGELNEVDPSFVARYGRVITRRGVAAVPRAVF
                     TRQAALGLTPQQ"
BASE COUNT          439 a          690 c          650 g          390 t
ORIGIN      
        1 gcacacccgc gataggctgc ggcacgggga tattggcctc cgagaagagc ttgccgacaa
       61 tcgtggaggt agccccgact ggttcgagcc gcatgaaagc gggactgcgc gtctggaaat
      121 cggccacgcg gtggtggtca ataaccccta tcacgtcggc ctcttcgata cccgccaccg
      181 attggctgcg ctcgttgtgg tcaaccagca ccacctgcct gcgcacctgc cgcagcagat
      241 tggtacggct gatgatcccc agcaccttct tgtccccatc cgtgaccacc agcgccctca
      301 ccctgcccgc ctgtagcgtc tcgcgcacct cctccaccag gtcatccggc tcacaggtaa
      361 ctacctcttt gcgcatcacg tggcgtacgg cggcgctcat ctggatgagg cgcacggtgg
      421 tgtaggtgtg gtgctctacc gatatgaccg ttacctggcg ctcgcgggcc aggtccagta
      481 tctcttcgga tacgggcgtg ccgccggtga gcacaagcgc gcccaccccg aactcgatgg
      541 ccgccctttg agcatcaatg cggtcgccca tcaccagcaa aatatcgggg gcgatgcgtt
      601 tgagcatgga gtctatctcc atagctccca ccatcacctg gtcgcgcaga gtgcggcccg
      661 gcgcggccac cagcagcttt cctcccaggg cgctcaacac gttgtccagg ttcaaatgca
      721 cgtggtccag ttggtcggag tcgaggccgc tgataaagct gcgggcgaag tcctcgatgc
      781 cggttatgcc gcacaggtgg ccctcgtcgt ccactacagg cacggcgcgc acgcccccct
      841 gctgcatgat ctggcccacc tcgtaaagag acgtctcggc atgcacagta gtcacggagc
      901 gcgtcattac gtccaccacg cgcgggcgca cgtcgtccac gaaaagcggc gcgggcacgc
      961 caaaacgctc caatatatat gcggtctcag ggcgcatctc tccctgccgg gcggctaccg
     1021 cgttggtacg cccttgcgcg tgcaacaggg ccgcatagcc aaccgccgag cagatagcat
     1081 cggagtcggg gttcttgtgg cccaacacgt agattgcggg taaaatcgtt tgcatctcag
     1141 aattatagct taagatggat gtatcgaaac gcgcgcaact atgctataat acttgtcata
     1201 atgacaaggg ggacatccgg tagggaggtg tggcagcctt cctcagcgtg aaggccccac
     1261 aggagtcagg ctagcagata agatcagcat aaccactaac taatacgaat agaacggtag
     1321 cgagagaaaa taaaaaacct gactgttggc gcaatcaggt ttcagaaatc tccgctgcta
     1381 cgggaccact tggcaggcga ccggcgtagc tatatttgtt tggcaaacac attgtagcac
     1441 ggtcaaaacc tcctgtcaag gggtcaggag gttttcctat gccgaaaaac aggggtcggg
     1501 gaacggggag cgaggggcga ggggcgagga tcgggggcca ggggtcaggc tctgcggcgc
     1561 acgcgcgggt ggatgacgtc gcgggacagg acgcgcagct tcgtggtgtg ccgggccggc
     1621 ccaggcatgg caggcagggg gggagtttcg ccgcccgctt cggcaggctc atcctccaac
     1681 acggcatcgc cgccatcccc agcgcgctct atcactacca gggcgagatg cagctatccg
     1741 cccagcaggt gtggctcgtc tcgtacatac tctcccataa gtgggacgag gaccttcctt
     1801 accccagcct caaaaagatg gcgcgctgca ccacgttgag cctcagccag ttgcagcgca
     1861 tcaagaacag cctgtgcgac atgggctacc tgaaggtcta cccgcgcttc gatgaaaagc
     1921 agggccagaa caccaacgcc tacgacttct ccggcctgtt cgaccgcctg gaagaactca
     1981 ttaccggcga cgcgcccgtc gccaacgaga tacgcgccga gagcgaaggc cccgacctcg
     2041 gagagcttaa cgaagttgac cccagcttcg tcgcgcgcta tggccgcgtc atcactcgcc
     2101 ggggggtggc cgccgtgccc cgcgcggtct tcacccgcca ggccgcgttg ggtcttaccc
     2161 ctcagcagg
//
LOCUS       DASRWZ010000518         1931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_522027, whole genome shotgun sequence.
ACCESSION   DASRWZ010000518 DASRWZ010000000
VERSION     DASRWZ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522027"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFH60_08045"
     CDS             <1..582
                     /locus_tag="VFH60_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6313770.1"
                     /translation="RAIGETRIVRATFDFLLSRPQDVRLSKELGGGSLLDVGCYCVNI
                     ANLVVGMPPKAVTASAVWSEAGVDMALVGTLEFPNGVLGAMDCSFQVGTSMQQKLEVS
                     GTGGLIQVTQPFRMGEEDTTIIIDRADKNGTIEQVRVPGGPEYQLMVEHFAGAVLTDT
                     DLSYTLEDSLANMRVLDALIEAARTGRRVEVGG"
     gene            886..1791
                     /locus_tag="VFH60_08050"
     CDS             886..1791
                     /locus_tag="VFH60_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6313771.1"
                     /translation="MTNIPPDFARTMQEVYGPAGADWLERLPALLAYCEQRWSLTVMP
                     PFALSYNYVAPALRNDGRRIVLKVGFPGAELMSEIEALLLYDGRGIAQLLDTDQERGV
                     LLLERLSPGTPLSRLTDDEQATSIAAGVMRLLWRPAPPEHPFPTVARWALGLGRLREH
                     FGGTTGPFPARLVEQAESLFAELLASAPEQALLHGDLHHDNILAAEREPWLAIDPKGL
                     VGEPAYEVGALMRNQLPQPLAGEYASRFLARRLDQLAEELGLERARLHGWSLAQAVLS
                     AWWSFEDHGYGWEPAMALAELLSGL"
BASE COUNT          356 a          580 c          653 g          342 t
ORIGIN      
        1 cgagccattg gcgagacccg catagtgcga gccacgttcg acttcctgct gagccgcccg
       61 caggacgtga ggttgagcaa ggagttaggc ggcgggtcgt tgctggatgt cgggtgctat
      121 tgtgtcaata tcgcgaacct ggtagtgggt atgccgccga aggccgtgac ggcaagcgcg
      181 gtctggtccg aggcgggcgt cgatatggcc ctggtgggta cgttggagtt ccccaacggc
      241 gtgcttgggg ctatggattg cagcttccag gtaggcacct cgatgcagca gaagctagag
      301 gtatcgggca cagggggact tatacaagtg acgcagccct tccgtatggg cgaggaagac
      361 acgaccatca tcattgatag ggccgacaag aacggcacta tcgagcaggt gcgcgtgccc
      421 ggcggacccg agtaccaact tatggtggag cacttcgcgg gcgcggtgct gaccgatact
      481 gacctgagct acaccctgga agacagcctc gccaacatgc gggtgctgga cgcgctgata
      541 gaggctgccc gcacggggcg tcgggtggaa gtgggcgggt agagaacggt gaggcagccc
      601 agcatggagg ccctgtgggg atgccctgcc aacagcgccc tgcatggacg ccctgtggct
      661 gaagccacgg gctgaccaga ggcaaagtcc cctacgggga ctgagggaac gtcatgtagt
      721 ctccgtagga gacttcgtcc cttgtaagcc cgccaattcc ttggcagggc gatgttggca
      781 gggcgatgtt ggcagggcga tgttggcagg gcgatgttgg cagggcgtca tgcacggcac
      841 ctcactgcct ccctgacaac aatgtctata ccccgctagg actatatgac caacatcccc
      901 ccggatttcg cccgcaccat gcaagaggtc tacggaccgg ctggtgccga ctggctcgaa
      961 cggctacctg ctctccttgc ttactgcgag cagcgatggt cgctaaccgt catgcccccg
     1021 ttcgcacttt cgtacaacta cgtcgcgccc gccttacgca acgacggcag gaggatagtg
     1081 ctcaaggtgg gctttccagg cgcggagctg atgtcggaaa tcgaggcgtt gctgctgtac
     1141 gatgggcgcg gcattgccca gcttctcgat acagaccagg agcgcggcgt gctcttgctg
     1201 gaacgcctca gtcccggcac gcccctttcg cgcctcacgg acgacgagca agccacctcc
     1261 atagcggcgg gtgtaatgcg cctgttgtgg cggcccgcgc cccccgagca cccctttccg
     1321 accgtcgcca ggtgggcgtt gggcctgggg agattgcgag agcacttcgg cggcaccacc
     1381 ggcccctttc cagcccggct tgtcgagcag gcggagtcgc tcttcgcgga gctgctagcc
     1441 tccgcccccg aacaggcgct cctgcacggc gacctgcacc acgacaacat cctggccgcc
     1501 gagcgcgagc cctggctggc gatagacccc aaggggctgg tgggcgaacc ggcctacgag
     1561 gtcggcgcgc tcatgcgcaa ccaactgccg caacccctcg ccggggaata cgcgagtcgc
     1621 ttcctcgccc gccgcctgga ccaacttgcg gaggaactgg ggctggagcg ggcgcggctg
     1681 cacggctggt cgctggctca agccgtgctg tcggcgtggt ggagcttcga ggaccacggt
     1741 tacggctggg agcctgcgat ggccctggcc gaactcctgt cgggcttgtg agagtggcgt
     1801 gcaggtaatg gcttcggtca gagacgaaaa cgcaaagacg caaagaacgc aaaggacgcg
     1861 cagagattaa ggatggttta ggtgggtgct aagaagacca gggtatgtta catgcaccct
     1921 ggtcctttta c
//
LOCUS       DASRWZ010000519         4608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1070204, whole genome shotgun sequence.
ACCESSION   DASRWZ010000519 DASRWZ010000000
VERSION     DASRWZ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070204"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..1097)
                     /locus_tag="VFH60_08055"
     CDS             complement(402..1097)
                     /locus_tag="VFH60_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6313772.1"
                     /translation="MGEWIKYIVGEKEGRGYLALPQQGRGPGVLLCHAWWGLNSFFQG
                     LAERLAKEGFVVLAPDLYDGKTAATIEEAEGLAYTLDYREAIKSETAAADYLLEHPAV
                     DGDRLGTVGFSMGAAYATWLATLRPQVSAVVVFYGGVEQEADYALRTRAAFLGHFAED
                     DPYESSEEMHRMAEMLRVARRDVTFHVYPGTGHWFFENDRPDAYNPEAAELAWQRSID
                     FLHGKLDQQPLPQ"
     gene            complement(1090..1425)
                     /locus_tag="VFH60_08060"
     CDS             complement(1090..1425)
                     /locus_tag="VFH60_08060"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6313773.1"
                     /translation="MAEYVYVIRATRAGFATEPTAEEQDAMGRHFLYLKGLVEQKKAL
                     LAGPCLDTAFGLVIYEADSEEEARRIMENDPSVIAGVMAAEMHEFRVSLLQRDYVPLR
                     ERKAETAVG"
     gene            complement(1444..2703)
                     /locus_tag="VFH60_08065"
     CDS             complement(1444..2703)
                     /locus_tag="VFH60_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET6313774.1"
                     /translation="MRDILERQANHIEYILRTHGIKAEIDGGKLSPRLAHFHVTLPQG
                     VRATTVAPIVPEIASELGVVSCRMTHGEEGVYLEVPRPDPVPVRLLQIVQRVADVVPP
                     ATATLGLDADGTPLLLRLNSPDVDPLLIVGNQGAGKSSLLGGMALSLALHNSPDRLRL
                     LLIDGTGEGRAFRGLERLPHLACPVANGPVDAMLSLRWALRAMARNARSIDPLALDDE
                     EEEDELFFGDEESALEPYVPHADTPSLVVLIDGADLLCGSGNRRAEAEAVSALNKLLQ
                     GGSRNHIHLALSVESLPMIDGLEADWGARIVGQVRSPEAARQATGIKSTGAHSLLGAG
                     DFLISLNTELIRFQAPGVSSSDIAKSVDLIVTCAQMQSEPEARSEYDEFDLTPMPVVP
                     ALSSRKQERVSHAAQEPVPLRRSWVGE"
     gene            complement(3043..3531)
                     /locus_tag="VFH60_08070"
     CDS             complement(3043..3531)
                     /locus_tag="VFH60_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6313775.1"
                     /translation="MDAQIDKATLLNNVRSGRSRLDALLATFTDEQIARAGAEGDWSV
                     KDVIAHVTWHEREIVKMLRARSLENGVASELWGLPTHERNRAIYEGNRDRSLQDVLAE
                     SRQVYAELDDLLAGLSEADLTDPGRYKDMPAEWQPWKIIAENTYEHYRDHVEVMGGTS
                     NE"
     gene            complement(3564..4274)
                     /locus_tag="VFH60_08075"
     CDS             complement(3564..4274)
                     /locus_tag="VFH60_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HET6313776.1"
                     /translation="MVISLRRQPHSTGPQLGPDPAWARFYRAGQTEGARRVKVQAIKP
                     DITSYRLLGDCPDELLEAVDRVRSRYNSSVYVARIDSARERPNANTVRLPVIGEIAAG
                     QYDVTVAYQEYDCLDYEGGIAVDKGMARNGAYALRVRGTSMTHVGIEPGDVVIVKPQS
                     WAEDGDFVVASYAGEAGGSEGLATLKRFYKRKDHVFLQSATASTDPIRLFPNRREGAH
                     EADPVRIQGIVTMVLKAG"
BASE COUNT          787 a         1548 c         1344 g          929 t
ORIGIN      
        1 gattttaggt tttagatttt ggattctatc ggtttgccgc caatccaaaa tctaaaacct
       61 aaaatctaca cgtcgtcccg ggttggatgc agaacttcgt tgggatcaac gcttctctaa
      121 ggctttctgc cgccgcaagc atgttgacgt gcggcttgcc tgaattggga gtgctaaacc
      181 tcgactcccc catgtaactg ttactcctgt agcgtccgtc gtccacttaa cacctccttt
      241 ctctcctgat tgggtaggca tcgcggggtt tcggcctcgg gctagcgaac gccccactcc
      301 cacatgccgt aatcaacttt cttgtcgagc cggtgccccg ttagaacctc tgtgcgcgca
      361 atgatgtatt caagcatatg ctcgccttgt atatgcgctc gctactgagg taaaggctgt
      421 tggtccagct tgccgtgcaa gaaatctatg ctgcgctgcc aggccagctc cgcggcctcc
      481 gggttgtagg cgtcggggcg gtcgttctcg aagaaccagt gacccgtacc gggataaacg
      541 tggaaagtca catcccggcg tgccacgcgc aacatctctg ccatgcggtg catctcttcg
      601 ctcgactcgt aggggtcgtc ctcggcaaag tgccccagga aggctgcccg cgtgcgaagc
      661 gcatagtcag cttcctgctc cacgccgccg tagaaaacca ccaccgcgct cacctgcggc
      721 cggagcgtgg caagccaggt agcataggcc gcgcccatcg agaagcccac ggtgccaagc
      781 cggtccccat ctacggcagg gtgctccaac aggtagtcgg cggcggccgt ctcggacttt
      841 atggcctctc gatagtcgag ggtgtaggcc agcccttccg cctcctcgat tgtggcggcg
      901 gtcttgccat cgtacaggtc tggagccagc accacgaacc cctccttggc cagccgttcg
      961 gccagcccct ggaagaagct attgaggccc caccaggcgt gacacagcag cacccccggc
     1021 ccgcgtccct gttggggtaa agccaggtag cctcgccctt ccttttctcc cacgatgtac
     1081 tttatccact cacccaactg cggtctccgc ctttctctcg cgcaacggca cgtagtctcg
     1141 ctggaggagc gacacgcgga actcatgcat ctccgccgcc atcacacccg ctatcacgga
     1201 ggggtcgttc tccataatgc gccgcgcctc ctcctcgctg tcggcctcgt agatgaccag
     1261 cccgaacgcg gtatccaggc acggtccggc cagtagcgcc ttcttctgct ccaccaagcc
     1321 cttcagatag agaaagtgcc tgcccatcgc gtcctgctct tccgccgtcg gctcggtcgc
     1381 gaagcccgca cgggtggccc ttatcacgta aacgtactcg gccatttccg tctccgtagt
     1441 ggcttattcg cctacccagc ttcgcctcag cggcaccggc tcctgcgcgg cgtgggacac
     1501 gcgctcctgc ttgcgagacg acagggcggg caccaccggc atcggggtca ggtcgaactc
     1561 gtcgtactcc gaccgcgctt ccggctccga ctgcatctgc gcgcaggtga caatcaggtc
     1621 caccgacttg gcgatgtcgg agcttgagac gccgggggcc tggaagcgta tcagctcggt
     1681 gttgagcgag atgaggaagt cgcccgcgcc cagcaggctg tgagcgccgg tgctcttgat
     1741 gcctgtggcc tggcgcgcgg cctccggcga gcggacctgc cccacgatac gcgcgcccca
     1801 gtcggcctcc aacccgtcga tcatcggcag gctctccacc gacaaagcca ggtggatgtg
     1861 gttgcggctg ccaccctgca acagcttgtt gagggccgat accgcctccg cttcggcgcg
     1921 cctgttgccg ctgccacaaa gcaagtcggc cccgtctatc agcacgacga gcgagggggt
     1981 gtccgcatgt gggacgtaag gctccagcgc gctctcctcg tcgccgaaga aaagctcgtc
     2041 ctcttcctct tcatcgtcca acgccagggg gtctatgctg cgggcgttgc gggccatggc
     2101 gcgtagcgcc cacctgagcg agagcatggc gtccaccggg ccgttggcta ccgggcaggc
     2161 caggtgcggc agccgctcaa gcccccggaa cgccctgccc tcccccgtgc cgtctatcag
     2221 cagcagccga agcctgtcgg gggagttatg tagggccagc gacagggcca tgccgcccag
     2281 caggctgctc ttgcccgcac cctggttgcc cactatcaac agcgggtcca cgtcaggcga
     2341 gttgagccgc aacagcaggg gcgtgccatc ggcgtcgagg ccgagagtag cggtggcggg
     2401 cggcaccaca tcggcaacgc gctgcactat ctgcaggagg cgcacgggca cggggtcggg
     2461 gcggggtacc tccaggtaca cgccctcctc accgtgggtc atgcggcacg agaccacgcc
     2521 cagctcgctc gcgatctccg gcacgatagg ggcaacagtc gtggcgcgca ccccctgcgg
     2581 cagggtcaca tgaaaatgcg ccagccgggg cgacagcttg ccaccatcta tctccgcctt
     2641 gatgccgtgc gtgcgcagta tgtactctat gtggttggcc tggcgctcca gtatgtctcg
     2701 catggttcgc tccgttagct gaaacgtggg agtggatagt ggtcggtggt cagtggatag
     2761 tggtcagtgg attcgcggcg gtctgtattc cggcggccaa gagtgaccct gtcatcctga
     2821 gcgtagcgaa ggatctaaga tgcccgcctt accgctacgc tgaaccctca acaccattgc
     2881 cctgacattc aacagcctcc ctactcacct tctttagatc cttcgctacg ctcaggatga
     2941 caggggcggg gcctggtccc gtaaggcaaa caacctgaca ttcacgcacc cactttagca
     3001 agccccatta cgagttacgg gttactcagt cctcagtcct catcactcat tactcgtccc
     3061 acccatgacc tccacgtggt cccggtaatg ctcgtaggtg ttttcagcga tgatcttcca
     3121 gggctgccac tcggcgggca tgtctttgta gcggccgggg tccgtcaggt cggcctcgga
     3181 gagtcccgcc agcagatcgt ctagctcggc gtacacctgc ctcgattccg ccagcacgtc
     3241 ttgcagggag cggtcccggt tgccctcgta gattgcccgg ttgcgctcgt gcgtcggcag
     3301 gccccacaac tccgaagcga caccattttc caacgaccgc gccctcagca tcttgacaat
     3361 ctcgcgctcg tgccaggtga cgtgggcgat aacatccttg accgaccaat cgccctccgc
     3421 cccggcccgc gcaatctgct cgtccgtgaa ggtcgccagc agcgcatcca gccgggagcg
     3481 gcccgacctg acgttgttca gcagcgtagc cttgtcgatc tgagcgtcca tcgcaggcaa
     3541 gtgctccttt gctcctttgc tccttagccc gccttgagca ccatcgtcac gatgccctgg
     3601 atgcgcaccg ggtcggcctc gtgagcgcct tctcggcggt tggggaagag gcgaataggg
     3661 tcggtggagg cggtagccga ctgcaagaat acgtggtcct tgcgcttgta gaagcgcttg
     3721 agggtagcca gcccttccga gccgcccgcc tcgcccgcgt aagaggccac cacgaaatcg
     3781 ccgtcctccg cccagctttg cggcttgact atcaccacgt cgcccggctc gatgcccacg
     3841 tgggtcatgg aggtaccgcg cacgcgcagg gcataagcgc cgttcctcgc catccccttg
     3901 tccaccgcga tgccgccctc gtaatcgagg cagtcgtact cctggtaggc cacggtcacg
     3961 tcatactggc ccgcggcgat ctcaccgatt acgggcaggc gcacggtgtt cgcgttgggc
     4021 cgctcgcgtg cgctgtcgat gcgggccacg tacacgctgc tgttgtagcg gctgcgcacc
     4081 ctatcgacgg cctccagcag ttcgtccggg cagtcgccca gtagacgata agaggtgata
     4141 tcgggcttga tggcctgcac cttcaccctg cgcgcgccct cagtttgccc cgccctgtag
     4201 aacctggccc aggccgggtc gggacccaac tgcggacctg tgctatgcgg ttgcctgcgt
     4261 agacttatga ccatgttgcc tccgaagaga tttgggattt ggaattgcga attgcggatt
     4321 tggggtccgt attcgctacc gtatcctctg gtttggtttg ccgccttacc agattactga
     4381 gactgtcctt ctaatcgtcc tatcgaatat gaaggtagca gcgggggaca ccccccgccc
     4441 cggcaggggt actcccctgc accctaactc cccaattcgg agttttccac acaatgtgga
     4501 aaactcaaaa cgcccctatc gaagcggaac cggccatgtg ccatgctgcc caggccggta
     4561 tagcttatcc ttggtgccgc gtgatacgca gcccacagtg atggattc
//
LOCUS       DASRWZ010000520         6777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1343932, whole genome shotgun sequence.
ACCESSION   DASRWZ010000520 DASRWZ010000000
VERSION     DASRWZ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343932"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFH60_08080"
     CDS             <1..243
                     /locus_tag="VFH60_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6313777.1"
                     /translation="PLWNHTLRTLSAVESSIINSTMLVQIALLAWLFLGEELTAQKIG
                     GMIMVGLGMLIVQLRRLPTRRRAADPAPTAEVPEQA"
     gene            complement(278..1654)
                     /locus_tag="VFH60_08085"
     CDS             complement(278..1654)
                     /locus_tag="VFH60_08085"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HET6313778.1"
                     /translation="MSAQSRPDYLQTAAVIAAQLCRDALWDGARCNWLGSSMEPVGNA
                     WTVVQRSFGPELYGGTSGIALFLAHAHQMTGERIFRKTAEGAIRHAESRIELDTPPNT
                     RVAFYSGYTGFAYVLIEAGAAFEDEELTGRGLRLLKALAEGDNTEQEVDIISGYAGAI
                     PALLAIYERHRQEFLVELAADLGEELLKRAAKSDIGWSWQTLGLPTLHDLTGFAHGAG
                     GIGHALLELNRATGQAQFLEAAEQAFRYERHWFSPAQENWPDFRNLDGGQQGETGANL
                     NYGVAWCHGAPGIGLSRVRAYSLLREGVYRTEAEAALRTVMRTLGPATGGWGNYSLCH
                     GVAGNAELLIYAARVLGDPSYKAAADAVGDRGMVQYGQQRMPWPCGVMGGGENPSLML
                     GLAGIGYFYLRLYDPDKVPSVLMLQPDLGKTSTPSASHNGRIGTKAQRRRDAKAQGTR
                     KKKKASGK"
     gene            complement(1651..2712)
                     /locus_tag="VFH60_08090"
     CDS             complement(1651..2712)
                     /locus_tag="VFH60_08090"
                     /inference="COORDINATES: protein motif:HMM:NF037672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector HopA1 family protein"
                     /protein_id="HET6313779.1"
                     /translation="MSAIETESVQGDLIQILRAVTIVSPEAFAFAGRLVSAADMPSYG
                     YPPGPGSAMGQAEANPLVAALQNHLYQYCYCVSFKNNRTDFTSPVSPDPGFVSSLSAA
                     NTSRERWDPGWQISAMIPGGQVVVQKGALVRAVWPGEFLMHGGTGMAPGVGAPVSLYM
                     PRESTVMQPGFYFAFGEAAGDWHDDGSHLRFYWNVKDSGAAALLSSITTQLNRFQVPF
                     RYKCLTAKEAYVRVDAAVLYVAKRLYRITAELLLDVQAQVAAQLDPETPLFTKQLAPG
                     LGFAEDPGVVESFGTSRCRLLAEGIWAAFVQGAQEERPRLQIVAQHFEKSGISLEAPY
                     LNPGSQDRYTFPTEGGLSS"
     gene            complement(2709..3803)
                     /locus_tag="VFH60_08095"
     CDS             complement(2709..3803)
                     /locus_tag="VFH60_08095"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6313780.1"
                     /translation="MILTPHNIVYYLLDSNLLTPESVVDGDFMVVEHSSRNRNFKVIR
                     RENTGYFVKQVRDPEPDAILTVRTEATCYWLAQNDPAFAALLPLMPRFYGFDSRRHVL
                     VTELLARSESLNEYHRRLGDFPPEIAVQLGKALAGYHRDVRPPADDSPHRNAFGRRLP
                     WILSAHQLAAGLQGSGANVQLLNIISRYPQFQQVLEGLQRGWQPTTLIHGDIKWDNCM
                     LSADPEGDLSIKVIDWEIADFGDPYWDVGAVFQAYLTFWIFSVPVAAQVPMAEALELA
                     QYPLEKMQPAMQAFWKSYLETRGVDGKEVRAVLRRAVQYGAARMLQTAYEYMQMATQV
                     SIDIICLLQVSLNILENPDEAIEHLLGIQV"
     gene            complement(4479..4883)
                     /locus_tag="VFH60_08100"
     CDS             complement(4479..4883)
                     /locus_tag="VFH60_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313781.1"
                     /translation="MSTGDPGVRVPAHKYVPANVGLWVPAHKYLPADVGVWLPAHKYV
                     PAHTLALPHNVSQHRAAAMPSDYNPASNVGLPGPTGRRRSAAYAGHGDASPGTRAYPA
                     DANLPHYSYKPAVPHHRHSLHTYSGMAVPYEV"
     gene            complement(4893..5186)
                     /locus_tag="VFH60_08105"
     CDS             complement(4893..5186)
                     /locus_tag="VFH60_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313782.1"
                     /translation="MSPNVGVRVPEHEYLPTADPYAWLPTHKYVPANVWVWLPAYQYV
                     PTPDQYARLPEHKYMSADIGVWVSEHEHLPANVGLWVSEHEHLPTADPYARVS"
BASE COUNT         1206 a         2099 c         2099 g         1373 t
ORIGIN      
        1 cccctctgga accacacgct gcgtaccctc tcggccgtcg agtcgagcat catcaacagc
       61 acgatgctgg tgcagatagc cctgctggcc tggctcttcc tgggcgagga gttgacggcg
      121 caaaagatcg gcggcatgat aatggtcggc ctgggcatgc tcatcgtgca actgcggcgg
      181 ctgcccaccc gacgccgggc cgcggacccc gcgccaaccg ctgaggtgcc cgaacaggcg
      241 taagggtatg cggcaccgcg tgccggtact cgtaccccta cttccccgaa gctttcttct
      301 tcttgcgcgt gccttgcgcc tttgcgtccc tgcgcctttg cgctttggtc ccgattcgcc
      361 cattatgtga cgcggaaggt gtacttgtct ttcccaggtc aggctgcaac atgagcacgg
      421 aaggcacctt gtcgggatcg tagaggcgca ggtagaaata cccgataccg gccaggccga
      481 gcatcaagct ggggttctcg ccaccgccca tcaccccgca cggccacggc atgcgctgct
      541 ggccgtactg gaccatgccc ctatcgccta ccgcgtccgc cgctgccttg tagctgggat
      601 cccccaacac ccgcgccgcg tatatcaaca gctctgcatt tcccgccacc ccgtgacaaa
      661 gggaataatt cccccaccca cccgtggcgg gacctagcgt tcgcatcaca gtacgtagcg
      721 cggcttcggc ttccgtccgg tacacgcctt cccgcaggag agaataggcg cgcacgcgcg
      781 acagcccgat gccgggcgca ccgtggcacc aggcaacccc gtagttcaag ttagcgcccg
      841 tttctccctg ctgcccgcca tccaggttgc ggaagtcggg ccagttctcc tgcgcggggc
      901 tgaaccagtg cctctcgtag cggaaggctt gctcggctgc ttcgaggaat tgagcttgcc
      961 cggtggcccg gtttagctcc agcagcgcgt gcccgatgcc acccgccccg tgcgcgaagc
     1021 cggtcaggtc gtgcagggta ggcagtccca gcgtctgcca cgaccagccg atgtcactct
     1081 tcgccgccct cttgagcaac tcctccccca ggtccgccgc cagctccacc aggaactctt
     1141 gcctgtgccg ctcgtagatc gccaggagag ccgggatagc ccccgcgtag ccgctgatta
     1201 tatccacttc ctgttccgta ttgtcgcctt ccgccagcgc ctttagcagc cgcaggcccc
     1261 gcccggtaag ctcctcgtcc tcgaaagctg cccctgcctc gatgagcacg tacgcaaacc
     1321 ccgtataacc cgagtagaag gccacgcgtg tgttgggtgg cgtgtctagc tcgatgcgcg
     1381 attcggcgtg ccggatagcg ccctcggcgg tcttgcgaaa tatacgctcg ccggtcatct
     1441 ggtgtgcgtg tgccaggaac agcgcgatac cgctcgtgcc gccgtaaagc tccggcccga
     1501 aagagcgctg caccaccgtc cacgcattgc cgaccggctc catggacgac cccagccagt
     1561 tgcaacgcgc gccgtcccac agggcgtcgc ggcacagttg cgccgcgatg actgccgccg
     1621 tttgcaggta gtccggtctt gactgtgcgc tcatgagctg aggccgcctt ctgtgggaaa
     1681 tgtgtacctg tcctgggagc cggggttcaa gtagggcgct tccaggctga tgccggactt
     1741 ctcgaagtgc tgagctacta tttgcaagcg cggcctttcc tcctgtgcgc cctgcacgaa
     1801 ggccgcccag attccttccg ccagcaggcg gcacctgctc gtgccgaagc tctccaccac
     1861 tccggggtct tcggcaaagc ccaggccggg ggccagttgc ttggtgaaca ggggcgtttc
     1921 cgggtcgagt tgggctgcta cctgtgcctg cacgtccaac agcaattcgg cggttatccg
     1981 gtagaggcgt ttcgcaacgt acaacactgc cgcgtccacc cgcacgtaag cctcttttgc
     2041 cgtcaagcac ttgtaccgga agggcacctg aaagcggttg agctgcgtgg taatgctgct
     2101 cagaagcgcg gccgctcccg agtctttgac gttccagtag aaccgcaaat ggctgccgtc
     2161 gtcgtgccag tccccggcgg cctcaccgaa cgcgaagtag aaccccggct gcatcacggt
     2221 ggactccctg ggcatgtaga ggctgacggg cgcgcccacg cctggggcca tgcccgtgcc
     2281 cccgtgcatc aagaactcgc ccggccacac ggcccgtacc agtgcgcctt tttgcactac
     2341 cacttgcccg cccggtatca tcgcgcttat ctgccacccc gggtcccacc tctcgcggct
     2401 cgtgttggcg gccgaaagcg aggacacaaa accagggtcg ggagagaccg gcgacgtgaa
     2461 gtccgtacgg ttgttcttga agctgacgca gtagcaatac tggtacaggt ggttttgcaa
     2521 ggcggcgacc agggggttgg cctctgcctg gcccatcgcg ctcccaggcc cgggtgggta
     2581 cccgtaggag ggcatatcgg ctgccgacac caggcggccc gcgaaggcaa aagcctccgg
     2641 cgacacgata gtcaccgcgc gcaatatctg gattaggtcg ccctggaccg attctgtctc
     2701 gatagcgctc atacctggat acccagcagg tgttcgatgg cctcgtcggg attttccagg
     2761 atgttgaggc tcacctgtag cagacaaatg atgtctatgg atacttgcgt ggccatctgc
     2821 atatactcgt aggcggtttg cagcatacgg gccgccccgt actggaccgc acgcctcaac
     2881 acggcgcgca cctccttgcc atcgaccccc cgcgtttcga ggtagctctt ccagaacgcc
     2941 tgcatggcgg gctgcatctt ttccagcggg tattgcgcca gttctaacgc ttcggccatt
     3001 ggcacctgcg cggcgaccgg caccgagaat atccagaagg tgagatatgc ctggaagacg
     3061 gcgcccacgt cccagtaggg gtcgccaaag tccgcgatct cccagtcgat gaccttgatg
     3121 ctcaggtcgc cttccgggtc tgccgagagc atgcagttgt cccactttat atcgccatgt
     3181 atcaaggttg tcggctgcca gccgcgctgc aaaccctcca ggacctgctg gaactggggg
     3241 tagcggctga ttatattgag caactggacg ttcgcgccgc tcccctgcaa cccggcggca
     3301 agctggtggg ccgaaagtat ccagggcagg cgacgcccga aagcgttcct gtgcgggctg
     3361 tcgtcggcgg gggggcgcac gtcgcggtgg tatccggcga gggctttgcc aagctggacg
     3421 gctatctccg gggggaaatc gccaaggcgg cggtgatatt cgttgaggct ttcgctccgc
     3481 gccagcaact ccgttaccag gacgtgccgc cgcgagtcga atccatagaa gcggggcatg
     3541 aggggcaaca gcgcggcaaa ggccgggtcg ttctgggcga gccagtagca agtagcttcg
     3601 gtgcgcacgg tcaatatggc gtcaggctca gggtcgcgca cctgcttgac gaagtagccg
     3661 gtattttctc tgcgtatgac cttgaagttg cggttgcggc tggaatgctc tacgaccatg
     3721 aagtcgccat ctacgaccga ttcgggcgtt agcagattgc tgtccagtag atagtagact
     3781 atgttgtgag gagtaagaat catccggggc aatcctctcc gggcaactgc taaccctggc
     3841 ctgccttatt gtagcagaat cacagagatt acccacggct atttgccata gcttagggta
     3901 atctctgtgg actagggtaa gtaacgtcag taattagcgt cccgagtgtc tatccgccga
     3961 acacggggcc tccacccccg ccccaacctg ggttgatggt ccaacccagg tcgatagtcc
     4021 aggggcaacc gagtgccgag ggacaggcgt caactgctga agggcagatg cgagtcgcct
     4081 ggcacaccgg gctattagtg gggcagaact gcgtcgcaca gatccaggtc tggcagacct
     4141 ggtgaattga ggggcagtta aactttgtgt gacatagcca cgctgccgtg ggtgtgcgag
     4201 ctatgccgag ggtgcggcac tgcgggtcta ggagtgtagg cacgacctgc gcttgtgcct
     4261 gggcttgcgc gccaaacatc tcctggccgg cttgtgccac gccgccaccg ccaacgccac
     4321 cctggtcgat tgtccagggg ccgcaaccga acctggttgg gcaggcgtca actgccgaag
     4381 ggcaggtgcg ggtcggccgg catatctggt tggtcgtggg gcagagttgc gtggggcacc
     4441 ccagccacga ttggcagatt tggtgagcgg tcgggcagtt aaacttcgta tggcacagcc
     4501 atgccgctgt aggtatgcaa gctatgccga tggtgtggca ctgcgggttt gtagctgtaa
     4561 tggggcagat tcgcgtcggc gggataggcc cttgtacctg ggcttgcatc tccatgccca
     4621 gcataggctg cgctcctccg cctgcctgtg ggtccgggca atccaacgtt ggaggcggga
     4681 ttgtaatcgg aaggcatggc ggccgcgcgg tgttgggaca cattatgggg aagcgcgagc
     4741 gtatgggcgg gcacgtactt gtgcgcgggc agccaaaccc cgacgtcggc gggcaagtac
     4801 ttgtgcgcgg gcacccaaag cccgacgttg gcgggcacgt acttgtgcgc gggcacccgt
     4861 acgccagggt ctccggtgga catgtactcg tgctaggaca ccctagcata tgggtctgcg
     4921 gtgggcaagt gctcgtgctc ggacacccaa agcccgacgt tggcgggcaa gtgctcgtgc
     4981 tcggacaccc ataccccgat gtcggcggac atgtacttgt gctcgggcag cctggcatat
     5041 tggtctgggg tgggcacgta ctggtaggcg ggcagccata cccagacgtt ggcgggcacg
     5101 tacttgtggg tgggcagcca agcatatggg tctgcggtgg gcaagtactc gtgctcgggc
     5161 acccgtaccc cgacgttggg ggacatgtac ttgtggacgg gcagcctggc atattcgtct
     5221 gcgggtcttc gccctgtcca ccgccaccgc cacctccggc tgcgccaccg aggtagtcct
     5281 gggcaattcg cccttcgaag aacctggctc tctggcggtt aggaccggct ttgccgtgga
     5341 ttagctcggc gtcgctctgt atccacacgt agcttgaccc cgcgagcgcg gacccttcgg
     5401 ctgccagcgg ctgcacgaac agtatggcgt cgttgggtat ctcgacgtag tcgctcagct
     5461 ccgcgtcgaa atagagacgg gtgtaatcag gctcggacga acggcccagg aagccggtca
     5521 agaggatcgt gtcgggtggg ttcgtcggat cgcccactat cttggcgata aagtcggcct
     5581 cagggcctcc caccggctcc gtcagttccc tgggcggctc tgccggcggc tcttcaggca
     5641 gcccttctac gggtccgcct gccgttcctc ctggcggttg atcgccttcc ggtggatcag
     5701 cgtctcgatt cggtttgttt ctctcagcca tagatttgcc tccgtgttac ttaacatagc
     5761 cccagctttg gggcaacatg cctacggaag tgggcctgta cagacgtaga gaaagaaaac
     5821 cctggccggt gccttcccgc cttcctgcct tttgcatgtg ctgcacttca ggcaatagag
     5881 gcgtaaagct cccaggaatt caatttccct tgtgcgccag actatatttg cgaccgtgcg
     5941 gccccggcgt aatctcgccg tttagcctgc cgcaaccagt cccgccaggc cgggtacgtt
     6001 ctgcgcgcta aagtcgccag taatatcttc tcctctgccc tcgcgattgt gcagcatcac
     6061 ctcctcgtcg gaaggcagga ccgagctaca acatacacat cgcccgtcca cttctgcctg
     6121 ctagcgccgg tgggccggtg gagcactgtc gcggtgtcct tccggcgggc gggtgagcgg
     6181 ccttcaagct tgccgtactc atcccgcggt atcgtcttaa tagcctcgga ttttgttgta
     6241 ttgcagttgc agtgtggatg cgggcgagaa tagaacgggt caagcgttat gaacggctaa
     6301 ggcttcaaaa tggatggcga caaaccacca tccgtaagtc tcaacccctg gtgacgagac
     6361 acggcgggcg ggcaagactt ccaaagaaag gcaggtagtt cagtacgtaa aatttagcta
     6421 ttcgtgcttg ttggcgcaca agaagagtga agcagaagta cattggtgtt ccgtcttaca
     6481 ggctctcgta cccctggggc attggcttaa gtaatatatc atagctggag gtctaaggca
     6541 atagcttatg tacccgaaga gtcttgtaaa ttggcgtgtg acttgaaggt cctgctaggc
     6601 agccgggggc cagagaaggc gaacgattgg gccgtgccat gtgcgtgcag tgaccgggct
     6661 tcacaacagc tatcgcgcct ctaccagggc ttaatcattc tccgcggctg gggtggaaat
     6721 gccctgtggc attgccctgc caatgaattg gcgggcttac aagaggcaaa gtcccct
//
LOCUS       DASRWZ010000521         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_110973, whole genome shotgun sequence.
ACCESSION   DASRWZ010000521 DASRWZ010000000
VERSION     DASRWZ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110973"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFH60_08110"
     CDS             complement(<1..227)
                     /locus_tag="VFH60_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET6313783.1"
                     /translation="MDRQKAGAGLKAADIPPGSCGLGRAGNEEVAVFNVEGQFYATQN
                     RCTHAGGPLCEGGLWGDVVQCPWHGSEFNVRT"
     gene            436..1617
                     /locus_tag="VFH60_08115"
     CDS             436..1617
                     /locus_tag="VFH60_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313784.1"
                     /translation="MITSSHANGETDDRLFDAWVREYIHLGLLMDRLEPGTVDSYYGP
                     ASWRTGMENDPLPTPETLRDAAEGLLSALPGMGYPAERQGFLERQVRALHTQARVLSG
                     ERIPLIEQAKLIFDINPERVPEEVFAQAHDMLDAALPGQGSLYERRNLWRKQFDVPTE
                     KLLPLLDKIASEVRSRTVREVPLPEGESIELSLVSDKPWGGYNWYLGDMRSLIEINTD
                     LPVSSLSLVDYLAHEGYPGHHTEHALREAVQYRQRGQGEYAITILNSPESLIAEAIAT
                     TARKLIFPGNSDLEWAAEHIFPELGIEADVEQIAQVREAQRDIGAVSGNASLLLNEDG
                     RSTDEVVQYLEQWGLRRHEEAQHSIRFLSSPLSRVYVFTYYYGERLLDPLLAGPDRFQ
                     V"
     gene            1773..2765
                     /locus_tag="VFH60_08120"
     CDS             1773..2765
                     /locus_tag="VFH60_08120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6313785.1"
                     /translation="MAIKFGVLVPQGWRMDLVDIQDPVEKYEAMTRVAKEAERFGFDS
                     VWLYDHFHTVPTPELEATFECWITTAALARDTSNIRIGQMVSCNSYRNPALLAKMAST
                     VDVLSHGRLDFGIGAGWYEHEYRAYGYPYPDGPERLRMLNEALQIFHAMWKEPYATFE
                     GKYYQVNGAINEPKGVQQPHIPIWVGGGGEKVTLKLVARYADACNVGGIDPANYRHKF
                     DVVRQHCENEGRDYGSIIKSAEITLNLLSPGQDAEEATRVLRERSGKAQNREIPLEEY
                     KRGNFVGSPQQCIDLLGSLVDAGVEYFIFYLPGVVHLEPLQILAEEVLPAFRSR"
BASE COUNT          580 a          973 c          870 g          513 t
ORIGIN      
        1 gtgcggacgt tgaactccga gccgtgccag gggcactgca ccacgtcgcc ccacaggccg
       61 ccttcgcaca gggggccacc cgcgtgggtg cacctgttct gcgtggcgta gaactgcccc
      121 tctacgttga agacggccac ctcttcgtta ccggcccgac ccagtccgca gcttccaggt
      181 gggatgtcgg cggctttgag gccagcgcct gctttctgcc tgtccataat gctatcctac
      241 gctagctaac gggagcttat ttcccctgat tggtccgcgg tgaacgccgt cccactatag
      301 gaccggcagc ccttatcggc acctgtatta tagcgcagaa ccagcccggt agagccatgt
      361 ggccgcggcc acaccctata agaaccgctg gtgttataat gccccattgc acttctaaca
      421 ccaggaggcc ctgccatgat cacaagctcc cacgccaatg gagagacgga cgaccgcctg
      481 ttcgatgcct gggtgagaga gtacatccac ctcggcttgc tgatggaccg cctggaaccg
      541 ggcaccgtcg atagctacta cggccccgcg tcctggcgca ccggcatgga gaacgacccg
      601 ctccccacgc ctgaaacgtt gcgagacgcc gccgaggggc tgttgagcgc gttgccgggt
      661 atgggctacc ccgccgagcg gcagggcttc ctcgaacggc aggtgcgggc gctgcacacc
      721 caggcaaggg tgcttagcgg cgagcgcatc ccccttatcg agcaggcgaa gctcatcttc
      781 gacatcaacc ctgagcgcgt gccggaggag gtgttcgccc aggcccacga catgctcgat
      841 gccgccctcc ccggccaggg ttccctttac gagcggcgca acctctggcg caagcagttc
      901 gacgtgccca ccgagaagct cctgcccctg ctggacaaga tagcctccga agtcaggtcg
      961 cgcacggttc gcgaggtacc cctcccggaa ggcgagtcta tcgagttgag cctggtgagc
     1021 gacaagccct ggggaggcta caactggtac ctgggcgaca tgcgctccct catcgagatc
     1081 aataccgacc tgcccgtgag cagcctcagc ctggtggact acctggcgca cgaaggctac
     1141 cccggccacc acacggagca cgcgctgagg gaagccgtgc aataccgcca gcgaggccag
     1201 ggcgagtacg ccatcaccat cctcaactcg cccgaaagcc tcatcgccga agccatagcc
     1261 accaccgccc gcaagctcat cttccccggc aacagcgacc tcgaatgggc ggccgagcac
     1321 atcttcccgg agctaggtat agaggccgac gtggagcaaa ttgcccaggt gcgcgaggcc
     1381 cagcgcgaca taggggcggt gagcggtaac gcctctctgc tgctcaacga ggatggccgc
     1441 tccaccgacg aggtggtgca gtacctggaa caatggggct tgcggcggca cgaggaggcg
     1501 cagcacagca tccgcttcct gtcgtccccc ctctcccgtg tctacgtctt cacctactac
     1561 tacggcgagc gtctgctcga cccgctcctt gcgggacccg accgcttcca ggtctaatac
     1621 accctagcca gcggatcggt ctacccgtcg ctgctggaag aaaggcgcaa ggagagagag
     1681 acaagttagc tccgcgctgg cactcatcct gccttcttcg tctttcatct tgtcgcgtga
     1741 gggtgcgaca ctcaaatcca ggaggttcga caatggcgat aaagtttggc gtgctggtgc
     1801 cgcagggttg gcgcatggac ctggtggata tccaggaccc ggtagagaaa tacgaggcga
     1861 tgacgcgagt tgccaaagag gccgagcggt tcggcttcga ctcggtgtgg ctctacgacc
     1921 acttccacac ggtgcccacc cctgagcttg aggccacgtt cgagtgctgg atcaccaccg
     1981 ccgccctcgc ccgtgacacc tcgaacatca ggatagggca gatggtgtcg tgcaatagct
     2041 accgcaaccc ggccctgcta gccaagatgg cctccaccgt ggacgtgctc agccacggca
     2101 ggctcgactt cggcattggc gcgggctggt acgagcacga gtaccgcgcc tatggttacc
     2161 cctatcccga cggccccgaa cgcctcagga tgctcaatga agccctgcaa atcttccacg
     2221 ccatgtggaa ggagccatac gccacgttcg agggcaagta ctaccaggtg aatggcgcta
     2281 tcaacgagcc gaagggcgtg cagcagcctc atatccccat ctgggtgggc ggtgggggcg
     2341 agaaagtcac gctgaaactc gtggcccgct acgccgatgc ctgcaacgtc ggcggtatcg
     2401 accctgctaa ctaccgccac aagttcgacg tggtaaggca gcactgcgag aacgaggggc
     2461 gcgactacgg cagcatcatc aagtcggccg agataaccct gaacctgctc tctcccggcc
     2521 aggacgcgga agaggccacc cgcgtgctcc gcgagcgcag cggcaaggcc cagaaccgcg
     2581 agattcccct cgaagagtac aaacggggca acttcgtcgg ctctccccag cagtgcatag
     2641 acctcctggg cagccttgta gacgcgggcg tagagtactt catcttctac ctccccggcg
     2701 tcgtgcacct ggagccgctg caaatcctgg ccgaagaagt gctgcccgcc ttcaggagcc
     2761 ggtagacagt tctaaccgga gacggaaccg ccaagacgcc aaggaccacg ccaagattaa
     2821 ggtttgttat tcaagtaaga ggaccagcat acgtcatcat gtacgctggt cctctttgcc
     2881 ctttcgaaac ctcttttctc ttggcgtggt ccttggcgtc cttggcgtct tggcgg
//
LOCUS       DASRWZ010000522         5640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_179772, whole genome shotgun sequence.
ACCESSION   DASRWZ010000522 DASRWZ010000000
VERSION     DASRWZ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179772"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFH60_08125"
     CDS             complement(<1..425)
                     /locus_tag="VFH60_08125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313786.1"
                     /translation="MNRSTQTLTNLLAVYSFDPGPATGRDRSGRAPLPAPGMLASIVV
                     VSQNSRHHLETCLNSLMYTVGLNTEIVVVDDASADGSAEYVAESFPWVRLLRNKTSTG
                     LASATNRGVSQAVGRYIVVLNAGTEVTSGWLDALLLPLQ"
     gene            complement(845..2398)
                     /locus_tag="VFH60_08130"
     CDS             complement(845..2398)
                     /locus_tag="VFH60_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6313787.1"
                     /translation="MEHVPDPNREPEGESGAGPEAPPLTPANPDVPLAPPPLPTRRIV
                     RPAAPADMPLQPDIRTSVVPGEEVPAGASPGIIVRPVAAPPPEIIAPVASQPHQEGAA
                     PREDLFSGISRNVFVLGVVSFFTDVASQMIVPLRILFLVTALQTPLALAGLIEGVVAS
                     AAGLVSIVSERLAERPGRRKPLMLLGYGLSSLVKPLLAFVASWQGALGLILLERAGNG
                     VRSGPRNALLADSTPAHIRGKAFGFHRGLDTLGAIVGPLVAFLVLLLTYDDVRAVLAW
                     TAVPGAIAVLVLLLFVRERRTGHVAVPAATEMPVQVEKVASPPLGKRFWMFTATSVVF
                     AFGNSSDAFIFLRTADLGDSLLLVPLVYFGFNVVYALFAGPLGKLTGRFGRLPVLLSG
                     YTVFALVYLGWAVAVEGWNAWALFLVYGIYAAATEGIGKAFVADMVPPEARATAFAWF
                     GGLVGLTAIPANLIAGWLWSTIGPSATFTFGAWSAAVAIALTLAWIPWLRSNTAPETL
                     FTAPQEANI"
     gene            complement(2403..3908)
                     /locus_tag="VFH60_08135"
     CDS             complement(2403..3908)
                     /locus_tag="VFH60_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313788.1"
                     /translation="MSSVSARELTREEAPQPAARSHGLDPLLSSVFLAAAALILALQN
                     PIYVELKPRPVESMVLCAVGIVLLAGANVLRNMPMLGAGSAVLLLVVAAGTMSQPAQL
                     AVPLIAAWPLRVGVVLLVAAAWAFLLQPPWWARRALLAFLLPSALLLLYWIVPPVVYP
                     MLGFRLQPIVNNKVPPYWIAVDRHGGVYATDLNGMLVWAFDPLGQPRGAMNLTKAPPT
                     PGPGPGFAPVGFGNEVDAIGSRLLRSTPTPSAGAAPVPRSAITAFDFCGIATDARDNL
                     YLADLFDPRGYKLLRLDLNGNLTARWDIPAEQLPTNDCLTVDSQYIYLSVLERGQKGR
                     ILVYDHGGNLVREIDLPFLPLSVSARDDWPAGDTRGRAILIMGQGGVQRLDLDGNDSN
                     LRLLFTPPPEYQVPMLLTSKGEVLLTNRQSLQVGRFNPDTGELLGTWGGGGSMPGLFG
                     DIGSLAEDSQGRIYVSDPVNGVIHRVATDGMVTAVLWAPMFGIPPAPIEID"
     gene            complement(4044..4424)
                     /locus_tag="VFH60_08140"
     CDS             complement(4044..4424)
                     /locus_tag="VFH60_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313789.1"
                     /translation="MSDTDQTLVDTWRGIIVGEGKAWVLFEHGTCVIFAGPAQDLEAQ
                     ATALMKEWGPVVAGTPAGDFNVVKLQDDPGWVVTSHHPDILTYVGADEFDEGQVPEDM
                     LIGLIGRSKRQQDAEELHVVHTES"
     gene            complement(4421..>5640)
                     /gene="thrC"
                     /locus_tag="VFH60_08145"
     CDS             complement(4421..>5640)
                     /gene="thrC"
                     /locus_tag="VFH60_08145"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET6313790.1"
                     /translation="EPLDIISDERPVVSRELLDGRLGERQSPLSSGVWRYREMLPAFE
                     DRHIISRPEGNTNLYQVGAGESGGLRRVGEYAGLERLALKHEGENPTGSFKDRGMTVG
                     VSQAVRTGARAVACASTGNTSASLASYAAQAGLPCFVFVPEGNIALGKLAQTLAYGAV
                     TLQVRGDFDSAMTLVQEACAELGIYLVNSVNPFRIEGQKSIGFELLQQLAWQPPDWIV
                     LPAGNLGNTSALGKALLQARDLGLIDRLPRIASVQASGANPFFQSYRSGFSERFTVTA
                     HTVATAIKIGAPVSFVRAQKAIEATNGLVMQVPDEAILDAKAVIDRAGIGCEPASAAS
                     LAGARQLVQEGVIGREARVACILTGHLLKDSDTTLDYHLGRAGAAGSYANRPVVVDSN
                     HDAVRLMKELLLS"
BASE COUNT         1039 a         1860 c         1808 g          933 t
ORIGIN      
        1 tcttgcagag gcaacaacag ggcgtcgagc cagccggacg tcacctcggt accggcgttc
       61 agcacgacga tgtaccggcc cacggcctgg gatacacccc ggttggtggc agaggcgagg
      121 cccgtgctcg tcttgttacg cagcagcctc acccacggaa aggactcggc cacgtactca
      181 gcgctcccgt cggctgaagc atcgtccact acgacaatct cggtgttcaa ccccacggta
      241 tacatgagcg agttgaggca cgtctccagg tggtggcggc tgttctggct gaccaccacg
      301 atgctggcga gcataccggg tgcgggcagg ggcgcgcggc cgctgcggtc cctccccgtc
      361 gcggggccag gatcgaaact gtacacggcc agcagattgg tcaaagtctg cgtgctgcgg
      421 ttcatgcgga agtactcctt gagagaggcg aggtagcgaa tgtctcgcca atatatgcta
      481 gcaagtgcga ccctacgccc ccgtccattg ggcgctccgt gcttctgaaa ggggcgtggg
      541 ccttacaaac ccatcctagc aacccaccac aagcggcaca acttcctgat gtcacctgtt
      601 gccggccctt tggtccccaa cttcaaaggc cgatggtccc attattgtcg gttgaggata
      661 ggagacgaaa acgcaaagac gcaaagaacg caaagaacgc gcaaagagga tgaaggttta
      721 gtgagtgcaa agagaaccag agtaggttat cggtactctg gttctttttg cacgaacaac
      781 aagcctaatc tctgcgcgtt ctttgcgtct ttgcgtcttt gcgttttcgt ccctccgcac
      841 ccgcctagat gttcgcctcc tggggcgcgg tgaataaagt ttccggggcg gtattggagc
      901 gcagccaggg tatccaggcg agggtaagcg caatcgccac cgccgccgac caggcaccga
      961 acgtgaaagt ggcggatggc ccaatcgtgc tccagagcca cccggctatc aggttggcgg
     1021 gtatggcggt aaggcccaca agcccgccaa accaggcgaa ggccgtagcc ctcgcttcgg
     1081 ggggcaccat gtcggccacg aacgccttgc ctatgccctc cgtggcggcc gcgtagatgc
     1141 cgtagacgag gaacagcgcc caggcgttcc agccctccac cgccacagcc caccccaggt
     1201 agacgagcgc gaagaccgta tagcccgata gcagcacagg caagcgccca aaacggccgg
     1261 tcagtttgcc cagcgggcct gcgaacaggg cgtagaccac gttgaacccg aagtatacca
     1321 gcggcaccag cagcagcgaa tcgcccaggt cggccgtgcg caagaagatg aaggcgtccg
     1381 aagagttgcc gaacgcgaac accaccgagg tcgcggtgaa catccagaac ctcttgccca
     1441 gcggagggct tgccaccttc tcaacctgga cgggcatctc ggttgccgca gggaccgcca
     1501 cgtgtcccgt gcggcgctct cggacgaaga gcagcagtac cagcacggcg atggcacccg
     1561 gcacggctgt ccaggcgagc acggcccgca cgtcgtcgta ggtcagcagg aggacgagga
     1621 aggcgaccaa ggggccgaca attgcgccca gcgtgtccag gccgcgatgg aagccaaaag
     1681 ccttgccccg gatgtgggcg ggcgtcgaat cggccagcag agcgttgcgg gggccgctcc
     1741 tcacgccgtt gcctgcccgt tcgagcaaaa tcagtccgag cgcaccctgc cacgaagcta
     1801 cgaaagcaag caggggcttt accaggctcg aaagcccata gccgagcagc atgagcggct
     1861 ttcgtctgcc cggacgctcg gccaaccgct ccgacacaat cgacaccagg ccggccgcgc
     1921 ttgccacaac cccctctatc agccccgcca gggccagcgg cgtttgcagc gccgtcacca
     1981 ggaacaggat gcgcaagggc acgatcatct ggctggctac gtcggtgaaa aacgacacca
     2041 cgcccagcac aaacacgttc cggctgatac ctgagaatag gtcctcgcgc ggggccgcgc
     2101 cctcctggtg gggttggctt gcaacggggg ctataatttc cggcggaggt gccgcgacgg
     2161 gtcggacaat gatgccgggg gacgctcccg cagggacctc ttcacctgga accactgagg
     2221 ttcttatatc cggctggagt ggcatatcgg caggcgctgc gggccgaact atgcgacggg
     2281 tgggcagcgg cggcggggcc aggggcacgt cggggttagc gggcgtgagt ggcggggctt
     2341 cggggcctgc tccgctctcg ccctccggtt cgcggttggg gtctggtacg tgctccatag
     2401 cgtcagtcta tctcgatggg tgcgggcggg atgccgaaca taggagccca cagcacggcg
     2461 gtcaccatgc cgtccgtcgc cacccggtgg atgacgccgt tcaccgggtc cgacacgtag
     2521 atgcggccct ggctatcttc cgcgagggag cctatgtcgc caaacaggcc cggcatgctg
     2581 ccaccgccgc cccaggtgcc gagcagctcg cccgtgtcgg gattgaagcg gcccacctgc
     2641 aaagactggc ggttggtgag cagcacctcc cccttcgacg taagcagcat cggcacctgg
     2701 tactcggggg gtggggtgaa caacagcctc aggttgctgt cgttgccgtc gaggtcgagc
     2761 ctctgcacgc cgccctggcc catgatgagg atcgccctgc ccctcgtgtc gccggccggc
     2821 cagtcgtctc gtgccgacac cgagagcggc aggaacggca ggtctatttc gcggaccaga
     2881 ttgccgccat gatcgtagac cagtatccgg cccttttgcc cgcgttccag cacgctcagg
     2941 tatatgtact gcgaatcgac ggtgaggcaa tcgttcgtgg gcagttgttc cgccgggatg
     3001 tcccagcggg ccgtgaggtt gccgttcagg tccaggcgca agagtttgta gcccctgggg
     3061 tcgaacaggt cggccaggta gaggttgtcg cgcgcatcgg tcgcgatgcc gcagaaatcg
     3121 aaggcggtga tggcgctgcg gggcaccggc gcggcacccg cggaaggcgt gggcgtgctc
     3181 ctgaggaggc gcgagccaat ggcgtccacc tcgttgccga atccgaccgg ggcgaaaccg
     3241 gggccaggtc cgggcgtggg cggggccttg gtcaggttca tggcacccct cggctgcccc
     3301 agcgggtcga aagcccacac gagcatgccg ttcaggtcgg tggcgtagac gccgccatgc
     3361 cggtccaccg cgatccagta gggcggcacc ttgttgttga cgatgggctg taagcggaac
     3421 cccagcatgg ggtacaccac gggaggcact atccagtaga gcagcaacag ggcgctcggc
     3481 aggaggaagg ccagcagggc gcgccgcgcc caccagggcg gctgcaaaag gaacgcccag
     3541 gccgccgcca cgagcagcac cacacccact cgcaggggcc acgccgcaat gagcggtacg
     3601 gccagttggg cgggctgcga catcgtgccc gccgccacta ccagcagcag gacagccgag
     3661 cctgccccca gcatcggcat gttgcgcagc acgttggccc cggcaagcag cacgataccc
     3721 acggcgcaaa gcaccatgct ctctaccggc ctcggcttta gctctacgta gatggggttc
     3781 tggagtgcca gtatcagggc tgccgccgcg agaaagaccg acgaaagtag cgggtctagc
     3841 ccgtggctgc gcgctgccgg ctgcggcgct tcttcgcgtg ttagctcacg cgcgctgact
     3901 gaagacataa ggaagatatg ttccgcgaac tacctggcgt tcggcatgcg aattgaggta
     3961 catgcgtaaa gggctacgcg cctgccgcga cctgattgct gagacagtat aaggaaagcc
     4021 accctccctt gcaaacggcc cgctcaggac tcggtgtgga cgacgtgtag ctcctcggcg
     4081 tcctgctgcc gtttggacct accgataagc cctattagca tgtcctccgg cacctgcccc
     4141 tcgtcaaact cgtctgcccc gacgtacgtg aggatgtcgg ggtggtggga ggtgacgacc
     4201 cagccggggt cgtcctggag cttgacgaca ttgaagtcac cggcgggcgt gcccgcgacc
     4261 accggccccc actccttcat cagggccgtc gcctgcgcct ccaggtcttg tgcgggaccg
     4321 gcaaagatga cgcaggtgcc atgctcgaac agcacccacg ccttaccctc gcccactata
     4381 attccgcgcc aggtgtcaac aagagtctgg tccgtatcgc tcatgacagc agtagctcct
     4441 tcatcagccg caccgcgtca tggttggaat ccacgaccac ggggcggtta gcgtaagacc
     4501 cggcggcacc cgccctcccc aggtggtagt cgagggtggt gtcgctgtct ttcagtaagt
     4561 ggccggtgag gatgcaggcc acgcgcgcct cgcggcctat caccccttcc tgcaccagtt
     4621 ggcgcgcccc cgccagagac gccgccgacg caggctcgca ccctatcccc gcgcggtcga
     4681 ttacggcctt cgcgtcgagt atggcttcgt ccggcacctg cattaccagg ccgtttgtgg
     4741 cctcgattgc cttctgagcg cgcacaaagc tgaccggggc acctattttt atcgcagtag
     4801 ccacggtgtg cgccgtgaca gtgaacctct cgctgaagcc cgacctgtag ctctggaaga
     4861 acgggttggc tccgcttgcc tgcaccgagg cgatgcgcgg caacctgtct atcaatccca
     4921 ggtcgcgggc ctgcaagagc gccttgccca gcgcggaggt gttgcccagg ttaccggcgg
     4981 gcagcactat ccagtcgggc ggctgccacg ctagctgctg caaaagctcg aagcctatgc
     5041 tcttctgccc ctcgatgcgg aacgggttga ccgagttgac caggtagatg cccagctcgg
     5101 cgcacgcctc ctgcaccagc gtcatggcgc tgtcgaagtc gccccgcacc tgcaaagtga
     5161 ccgccccgta ggccagcgtc tgcgccagct tgcccagcgc tatattgccc tccggcacga
     5221 acacgaagca gggcagcccc gcctgggccg cgtacgaggc gagcgaggcc gaggtgttgc
     5281 cggtggaggc gcaggccacc gcacgcgccc ccgtacgcac cgcctggctg acaccgaccg
     5341 tcatgccccg gtccttgaaa ctgccggtgg ggttctcccc ctcgtgcttc agcgcgagcc
     5401 gctcgaggcc cgcgtattcg cccacgcgtc gcaggccgcc gctctccccc gcgcccacct
     5461 ggtagaggtt ggtgttaccc tccgggcggg agatgatatg ccggtcctca aaagcgggca
     5521 acatctcccg gtagcgccac acgccgctgg aaaggggcga ttgccgctct cccagcctgc
     5581 catccagaag ctcccggctg accaccggcc gctcgtcgga aattatgtca agtggttcgc
//
LOCUS       DASRWZ010000523         3610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_386037, whole genome shotgun sequence.
ACCESSION   DASRWZ010000523 DASRWZ010000000
VERSION     DASRWZ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386037"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3610
                     /locus_tag="VFH60_08150"
     CDS             <1..>3610
                     /locus_tag="VFH60_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313791.1"
                     /translation="RFGSFSFGSAGLLKSTDLGATWQIKGADVFRMPLPQPPGIFPQY
                     DSIGKVAVDPRNSNNLIVGSKRGVFFSYDQGDNWTGPCLPDPFPNQRQDITAIQTISS
                     TNVTELFVAVGSRGYSTTVQYNLAENGANGIYTTTVPLSGCPATWSLVSRPNNGWPPG
                     SGSGVPQYQPGGNVLGRIDLAVAPSNPNYIYAEVSAIRPGFGAIQTGGLLGVWRSTDR
                     GATWQQRVTAQDLEDAQDECGGNCVGGDPLGVCGDIAQNWYDQHIAVDPNDPNVIFFD
                     NVDVWKSTDGGDNIRNVTCGYSTIQVPRPVHVDQHAITFLPGSSTKALFGNDGGIYLS
                     ESVNLTQPTFIQLNTSLSTIEFYGGDISANFATSLAPFAVAGAQDNGSSSWQAADPSV
                     GPYLWQQRIGGDGMFARIEPVLGQRVYMEAQVGAMRLSTSGHQGPYPLLSAETNYTLD
                     APRLSFIFPYEIYKGVPAGAGGGEDCPPTGCTHLIGGTFRVWENTGGALPENFWYVNS
                     PDLTKNTLADRSFINQLAYAPRTDDIAIVGTNDGNVWIGFGLGNGAQNPASWANVTGG
                     NAVLPNRPILDVALDGAVMMTTTMPVGYAAVGGFDENTPGTPGHVFKITCTAANCTSF
                     TVENKSGNLPNVPADSIVGNPKFRQQVFVGTDWGVYYTNNIDAATPEWFRFNAGMPNV
                     MVWDFAIDRGFTTLAAFTRSRGVFVWPLPSAPFVGPTPTGTITTVTPTSTLGPPNTPV
                     VAATYTVNPSNPASPTRTATPIPPTITPGGPSLTPTPTIGAEIVLYDQYNRHGVLGSR
                     SQRYNGAPSDSRNLQLADDFVVPSGQQWIVTSVNVDGFYNGTGANAGSVNVFFYRNSI
                     TGTLPGAPVITMTIVPQDADGAFRLNLSPAVSLNAGTYWVSVQANLVDTRGWFWYNRR
                     PPLANNPAAGRQPNSAALNNACRDWNNRGVCVPGSEAEPDQVFLLSGYLIGGGGTATT
                     TALATTTALATGTPVATVVTGTATLVASTATSTVPAATTTGTPSLPTGTVVVATSTGT
                     SLATAVPGTGTATPGVPTLTATVSAPTQTVIAQTGTPTVALPTASTTTVAATQTPVGP
                     TTTLTAAAPTSTRTVVATLTPVGATNTVVVSTATRTITPIQPTGTSTVVLPSSTTTSL
                     STATRTSTTVAATGTSTRTSTAVSTATRTGTATAPSPTNTVAQPTTTVTQPTGTVVQ"
BASE COUNT          748 a         1251 c         1012 g          599 t
ORIGIN      
        1 cgctttggct ccttctcctt cggcagcgcg ggcctgctca agagcaccga cctcggcgcg
       61 acctggcaga taaagggcgc ggacgttttc aggatgcctc tcccccagcc gcccggcata
      121 ttcccgcagt atgactccat aggcaaggtg gcggtagacc cgcgtaacag caacaacctg
      181 atcgtcgggt cgaagcgcgg cgttttcttc tcctacgacc agggcgataa ctggaccggg
      241 ccttgcctgc ccgacccctt ccccaaccag aggcaagaca tcaccgccat acagactata
      301 tcgagcacca acgtgacgga gcttttcgtg gcggtgggtt caagaggtta cagcacaacg
      361 gtgcagtata accttgcgga gaacggcgcg aacggcatct acactacgac ggtgcctctg
      421 agcggctgcc cggccacatg gagcctggtt agccgcccga ataacggttg gcccccaggc
      481 agcggcagtg gcgtacccca gtaccagccg ggcggcaacg tgcttggccg cattgacctg
      541 gccgtcgcgc cgagcaaccc caactacatc tacgccgaag tgagcgcgat caggcccggc
      601 ttcggcgcta tacagacggg cggcctgctc ggcgtgtggc gctccactga caggggcgcg
      661 acctggcagc aacgtgtcac cgcgcaggac ctcgaagacg cgcaggacga atgtggaggc
      721 aactgcgtgg gtggcgaccc gctgggcgta tgcggtgaca tcgcgcagaa ctggtacgac
      781 cagcacatag ccgttgaccc gaacgacccc aacgtcatct tcttcgataa cgttgacgtc
      841 tggaagtcca ccgatggggg cgataacata aggaatgtca cctgcgggta cagcaccata
      901 caggtgcccc gccccgtaca cgtggaccag cacgctatca ccttcctgcc aggctcgtct
      961 accaaggcgc tcttcggcaa cgacggcggc atctacctga gcgagagcgt caatcttacg
     1021 cagcctactt tcatccagct caacaccagc ttgagcacca tagagttcta cgggggcgac
     1081 atcagcgcca acttcgcaac gtcgctagcg ccgttcgccg ttgcgggagc gcaggacaac
     1141 ggctcgtcct cctggcaagc cgcagatcca agcgttggac cctacctgtg gcagcagcgc
     1201 atcggcggcg acggtatgtt cgcccgcatc gagccggtgc tggggcagcg cgtctacatg
     1261 gaagcgcagg tcggcgcgat gcggctgtca acttcgggcc atcaaggacc ctacccgttg
     1321 ctgtcggcgg agacgaatta tacgctcgat gcgccgcgcc tcagcttcat cttcccctac
     1381 gagatatata agggcgtgcc tgcgggcgcg ggcggagggg aagattgccc gcccacgggt
     1441 tgcacccacc tgatcggcgg cacattccgc gtgtgggaaa acacaggcgg cgcactgccg
     1501 gagaacttct ggtacgtcaa cagccccgac ctgaccaaga acaccctggc ggaccgttcg
     1561 tttatcaacc aacttgcata tgcgccgcgc accgacgata tagctatcgt gggcaccaac
     1621 gacggcaacg tctggatcgg cttcgggtta gggaacggcg cgcagaatcc cgctagctgg
     1681 gcgaacgtca ccgggggcaa cgccgtactg ccgaaccgtc cgatactgga cgtggcgctc
     1741 gatggcgcgg ttatgatgac taccaccatg ccggttggct acgcggcggt cggcggcttc
     1801 gacgagaaca cgccaggcac gccgggccac gtattcaaga tcacctgtac agcggcgaac
     1861 tgcacttcgt tcactgtcga gaacaagtcg ggcaacctcc ccaacgtacc ggcagacagc
     1921 atagtgggca accccaagtt ccggcagcag gtcttcgtgg gcacagactg gggtgtctat
     1981 tacaccaaca acattgacgc ggcgaccccg gagtggttcc gcttcaacgc gggtatgccc
     2041 aacgtcatgg tttgggactt cgctattgac cggggcttca cgaccctggc ggcgtttacc
     2101 cgcagccggg gagtcttcgt gtggcctctg ccctcggcac ccttcgtggg acccacgccc
     2161 acaggtacaa taacgaccgt cacacccacc agcacgctgg gaccgccgaa cacccccgtc
     2221 gtggcggcta cttacaccgt caacccttcg aaccccgctt cccctacgcg caccgctacg
     2281 cccataccac ccaccatcac gccgggcggc ccatcgctga cacccacccc cactataggc
     2341 gctgagatcg tgctctacga ccagtacaac cgtcacggag tgctcggctc ccgttcgcag
     2401 cgatataacg gcgctccatc tgatagtcgc aacctccaat tggcggacga tttcgtagtg
     2461 ccttcgggtc agcaatggat agtcaccagc gttaatgttg acggcttcta taacggcacc
     2521 ggggccaacg caggctcggt aaacgtcttc ttctacagga actccatcac aggaacgctg
     2581 ccgggcgcgc cggtgatcac catgaccatt gtgccgcagg atgcggacgg cgctttccga
     2641 ctcaacctca gccccgccgt atccctcaac gcgggcacct actgggtatc ggttcaggct
     2701 aacctggtag atacgcgcgg atggttctgg tataaccgcc gtccaccact cgcgaacaac
     2761 ccagccgccg ggcgacaacc gaatagcgcg gctctcaaca atgcgtgcag ggactggaac
     2821 aaccgagggg tgtgcgtgcc aggctccgaa gccgagccgg accaggtatt cctgctgagc
     2881 ggctacctca taggcggagg cggcacagcc accaccacag ctttagcaac cactacagcg
     2941 ctggctacgg gcacacctgt agctacggtg gtcacgggca cggctacact cgtggcatcc
     3001 acggccacaa gcaccgtgcc ggcagcgact actaccggta ctccctcgct gccgaccgga
     3061 acggtggttg tagccacgag caccgggaca agtctagcta ctgcggtacc aggtacaggc
     3121 acagccactc ctggcgttcc cacgcttacg gcaacggtaa gcgcccccac ccaaacagtc
     3181 atagcgcaga ccggtacccc gaccgtcgca cttccgaccg cctccaccac aaccgtcgcg
     3241 gctacccaga cgccggtagg accgaccacc acgctcaccg cggcggcccc tacctctaca
     3301 cgcaccgtcg tggctactct gacacctgtt ggggctacaa acacggtggt tgtctccacc
     3361 gctacccgca cgattacacc catacagcct acgggcactt caacagtagt cttgccctcc
     3421 tccacaacca ccagcctatc cactgctacg cgcacctcga ctacggttgc agccaccggc
     3481 acctcaacac gcacgtcaac cgcagttagt acggctacgc gcaccggcac cgcaacagcc
     3541 cccagcccga ccaataccgt ggcgcagcca accaccaccg tgactcaacc tactggcaca
     3601 gtagtacaac
//
LOCUS       DASRWZ010000524         4017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1276450, whole genome shotgun sequence.
ACCESSION   DASRWZ010000524 DASRWZ010000000
VERSION     DASRWZ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276450"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2251
                     /gene="argS"
                     /locus_tag="VFH60_08155"
     CDS             <1..2251
                     /gene="argS"
                     /locus_tag="VFH60_08155"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET6313792.1"
                     /translation="EAVASLGYAASGLDLAIKPIPLEGAWGFGSTVAFQMRKLGVTGS
                     PQDVAQRIREALSNLPAVERVEVVGAYINFYIDRDWYANRIVSQVLAQGEAYGCWPDR
                     GERVMVEYANLNTHKAMHVGHLRNVVLGATIYNILKCAGFDAVSATYLGDIGMHVIRT
                     LWAYQRFYQGQEPEAETERGPWLETLYVDATARLEYRNNVTGLITDAARSDPSIAGQL
                     TLVLRALAENDLENGPYAAQIAESLTTREGPDWKLVLAERDHIVLNLWAALGPLLIGQ
                     TSTLDSTNRDEAYAQPTEPVVPQFLMDSMQDAADEGDNTTIETNLPIADDPLSGLRAE
                     YARLDDHREWWDHVPGWRDEIRRMFASWEAQEPELVQLWNTTREWSLAMFRRIFGDLG
                     IDFHVWFFESEVEEPGKDVVKDLIERGIAEDLRPEGPVIVRVDDQLRAHPELSKKYEK
                     DLWKTNKDGTREPRDAWRVLVVLRSDGTSLYATKDLELARRKFNDWGVDRSIYVIDSR
                     QSLYFQQVFRVLELWGFKQADKSFQLSYEFVNTPEGAISSRKGGAPQYEDLEAEALAR
                     ARNVVDAREEEQPRDLSDEQRDAIARAVAYGALKMGMLDKDNNKVITFVWEQALNPES
                     QSATYVQYSHARATRVLEKAPAALLPRPGEHYNFRELELQETSLLELIAKFPEEVARA
                     AEAYKPVIIAMYCFDLADAFNNFYHNCPILRAGGEEKVAARLALTAVAKQTLANALGL
                     LGIDAPEMM"
     gene            2518..>4017
                     /locus_tag="VFH60_08160"
     CDS             2518..>4017
                     /locus_tag="VFH60_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HET6313793.1"
                     /translation="MAGKVAMLQITSEFGRLREVIVHRPGQELVRMTPSTRDYFLFDD
                     LLYNEHARQEHDWLTDLLRDHLGVKVHYFEEMLYEALSLAPSEQRNALLEQVCSMESG
                     RPPVEQRIGQLEVMVRWFRGRGWPQYTHDPEGDPMSGAPGSIASALSSVSTRTRNTAP
                     LSAVDLESSDYLERRLGGWCAQGDYHALAEELVQGIESGVAGTSPTERPGPEANGNRQ
                     RLIDADKDALRDFTEGRLFFLTPLPNLMFMRDVGTVVGKRFLLSRMAAPGRAREPLLL
                     DFVQRHHPRFQGTTRWTWADTRISDPAWPLASSPKLMLECGNVMQLREDLIVLGVSER
                     TSMDAVQRLSDAWREHAASHNRRMVLYVLRLPAGFNHLDSVFGMVSENECVIYPPVFE
                     PYGPASVDVVRAELGKEPVHPTRSADFFKSLRADGLDLHRIACGGDDSVDQQREQWFS
                     ASNLLAVAPGKVVIYRSSEKTVGELARRGYRVVDINDVQTGATTLSLDDP"
BASE COUNT          741 a         1279 c         1324 g          673 t
ORIGIN      
        1 ggaagctgtt gcgagcctcg gttacgctgc ttcggggctg gacctggcga tcaagccgat
       61 cccgctggag ggggcctggg gcttcggctc caccgtggct ttccagatgc gcaagctggg
      121 ggttacgggc agcccacagg acgttgccca gcgaatccgt gaagcgctga gcaacctccc
      181 tgccgtggag cgtgtggagg tggtaggcgc ttacatcaac ttctacatcg acagggactg
      241 gtatgccaac cgcatagtta gccaggtgct cgcgcagggg gaggcgtacg gctgctggcc
      301 ggacaggggc gagcgggtga tggtagagta cgccaacctc aacacgcaca aagcgatgca
      361 cgtcgggcat ttgcgcaacg tggtgcttgg ggcgaccatc tacaacatcc tcaagtgcgc
      421 cgggttcgac gcggttagcg ccacgtacct gggcgacatc ggtatgcacg tgatcaggac
      481 gctgtgggcc taccaacgct tctaccaggg ccaggagccc gaggcggaga cggagcgcgg
      541 cccctggctt gaaaccctgt acgtggatgc caccgcccgg cttgaatacc gcaacaacgt
      601 taccggcctc atcaccgacg ccgcccgctc ggacccctcg attgccgggc agttgaccct
      661 ggtgctgcgc gccctggccg agaacgacct tgagaacggc ccctacgccg cccagatagc
      721 ggagagtctc accacgcggg aaggccccga ctggaagttg gtgctggccg agcgcgacca
      781 tatcgtgctc aacctgtggg ctgccctcgg cccgctgctg ataggccaga cctcaactct
      841 cgatagcaca aaccgcgacg aggcctacgc tcagcccacc gagccggtag tccctcagtt
      901 tctcatggac agcatgcagg atgccgcgga cgagggcgac aacactacca tcgagacgaa
      961 cctgcccata gccgacgacc ccttgagcgg cttgcgcgcc gagtacgcgc ggctggacga
     1021 ccaccgcgag tggtgggacc atgtgccggg ctggcgcgac gagatccgcc ggatgttcgc
     1081 aagctgggag gcccaggagc cggagcttgt gcagctatgg aacaccaccc gcgagtggag
     1141 cctggcgatg ttcaggcgca tctttggcga cctgggcata gacttccacg tctggttctt
     1201 cgagagcgag gtggaggaac cgggcaagga cgtggtgaaa gacctcatcg agcggggcat
     1261 agccgaggac ctgcggccgg aaggcccggt catcgtgcgc gtggacgacc agttgcgggc
     1321 gcaccctgaa ctgtccaaga agtacgagaa agacctctgg aagaccaaca aggacggcac
     1381 gcgagagccg agggacgcct ggcgcgtgct ggtggtgttg cgctcggacg gcacgtcgct
     1441 ctacgctacc aaggacctcg agcttgcccg ccgcaagttc aacgattggg gagtagatcg
     1501 ctcgatatac gtgatcgaca gccgccaatc gctctacttc cagcaggtgt tccgcgtgct
     1561 ggagctatgg ggcttcaagc aggccgacaa gtcgttccag ctatcgtacg agttcgtcaa
     1621 cacgcctgag ggtgctatct cgtcgcgcaa gggcggggca ccccagtacg aggacctcga
     1681 agccgaggcg ctggcccgcg cgcgcaatgt ggtggacgcc agggaggagg agcagccccg
     1741 cgacctcagc gacgagcagc gggacgcgat tgccagggcg gtggcctatg gtgcgctcaa
     1801 gatgggcatg ctcgacaagg acaacaacaa ggtaatcacc ttcgtgtggg agcaggccct
     1861 caatcctgaa agccagtcgg ccacctacgt gcagtactcc cacgcccgcg ccacccgcgt
     1921 gctggagaaa gcgcccgccg cgttgctgcc caggccgggc gagcactaca atttcaggga
     1981 gttggagcta caggaaacgt ccctgcttga gcttatcgcc aagttcccgg aggaggtggc
     2041 ccgcgccgcc gaggcgtaca agccggtgat tatcgctatg tactgcttcg acctggccga
     2101 cgccttcaac aacttctatc acaactgccc cattctgcgg gccggcggcg aagagaaggt
     2161 ggccgccagg ctggccctca ccgccgtcgc gaagcagacg ctggcgaatg cgctggggct
     2221 attggggata gacgcgccgg aaatgatgta agattgtgtg ggcgcggggg ttgcgcccgc
     2281 cgggttgccc tgtggctaaa gctacgggct gagaaggggc aaagtcccct acggggacta
     2341 aggaacgtca tgtagtctcc ccgtctccga aggagacttc gctacttccc agcccgtggc
     2401 ttcagccaca gggcgccatg caaggcgtca ccggcggact cgtccgcatt ctcaccagta
     2461 ccctcacccg tacacgatag agcaggccga caaagattct agcaatgcca cgttgatgtg
     2521 gccggtaagg ttgcgatgct gcaaataact tcagaatttg gccgcctccg agaggtaatc
     2581 gtccatcgcc cagggcagga actggtgcgg atgaccccct ctacccgcga ttacttcctg
     2641 ttcgatgacc tcctctacaa cgagcacgcc cggcaggagc acgactggct cactgacctg
     2701 ctgcgggacc acctgggcgt gaaggtccac tacttcgagg aaatgctcta cgaggcgctg
     2761 tcgctagcac cttccgagca gcgcaatgcc ttgcttgagc aggtgtgctc catggagtcg
     2821 ggcaggccgc cggtggagca gcgcatcggg cagcttgagg tgatggtgcg ctggttccga
     2881 gggcgaggct ggccccagta tacccacgac cccgaaggcg acccgatgtc gggcgctcct
     2941 ggctctattg cctcggccct ctcttcggtg tctactcgca cgcgcaacac cgctcccctt
     3001 agcgccgtgg acctggagtc gtccgattac ctggagcggc ggcttggcgg ttggtgcgcc
     3061 cagggggact atcacgcgct ggccgaggag cttgtccagg gcatcgagtc gggcgtggcc
     3121 gggacttcgc ccaccgagag gcccggaccg gaggcgaacg gcaaccggca gcgcctcatc
     3181 gacgccgaca aggacgccct gcgcgatttc accgaggggc ggctcttttt ccttaccccc
     3241 ttgcccaacc tgatgttcat gcgcgacgtg ggcacggtgg tcggcaagcg gttcttgctg
     3301 tcgcgcatgg cggcccctgg acgcgcccgc gagccgctcc tgctcgactt cgtgcagcgg
     3361 caccacccgc gcttccaggg caccacacgt tggacctggg ccgacacccg catcagcgac
     3421 cccgcctggc ccctcgcctc ctcgcccaag ctgatgctgg agtgcggcaa cgtgatgcag
     3481 cttcgggaag acctgatcgt gctgggggtg agcgagcgca ccagcatgga cgcggtgcag
     3541 cggctctccg acgcgtggag agagcacgcc gcctcccaca atcgccgcat ggtgctgtac
     3601 gtgttgcgcc tgcctgcagg gttcaaccac ctcgactcgg tgttcggcat ggtcagcgag
     3661 aacgagtgtg tgatctaccc tcccgtgttc gagccgtacg gcccagcctc ggtggacgtg
     3721 gtgcgggcgg agcttggcaa ggagccggtg cacccaaccc gctccgccga cttcttcaag
     3781 agcctacggg ccgacgggct ggatctgcac aggatcgcgt gtggcggcga cgactcggtg
     3841 gaccagcagc gcgagcagtg gttcagcgcc tccaacctgc tggcggtggc ccccggcaag
     3901 gtggttattt accgctcgtc cgagaagacg gtgggggagc tagcccggcg cggctacagg
     3961 gtcgttgaca taaacgacgt gcagaccggc gctacgactt tgagcctgga cgacccc
//
LOCUS       DASRWZ010000525         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_111280, whole genome shotgun sequence.
ACCESSION   DASRWZ010000525 DASRWZ010000000
VERSION     DASRWZ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111280"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VFH60_08165"
     CDS             <1..1082
                     /locus_tag="VFH60_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313794.1"
                     /translation="LVAGDPGSDAPSYAALQGVASVGLPGSDRRVPPVAPGVVLPSQY
                     IDHNGAVSRNHPALVGAEALKSAGFANETGHNIPDVFWSYLNAEGPVFVSNGYVNDRL
                     VDWVTAFGYPVTEPFWTTIKVAGRDRLILFQAFQRRILTYSPANAVGWKVEMGNVGAQ
                     YYSWRYETPAVTCQRVPVRGFGRVWADNRNVQRDLGCPLPYPPFDKEISVKTAFQPFE
                     HGSMLYVERTTYTQERIIYVFFDDGTFQPFEDTWREGQPVNGGLTPPQGRYEPVRGFG
                     KVWREGTGARVRERLGWATATEKGADGAYQRFERGEMYWSGTVDKIWALFNSVPPGGF
                     ADPTPAPGGGLPPLRYLVFDDTFNP"
     gene            complement(1169..1609)
                     /locus_tag="VFH60_08170"
     CDS             complement(1169..1609)
                     /locus_tag="VFH60_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313795.1"
                     /translation="MDNEDQFLSQVLSGISEAEVVTIFFPLLRRAMVIDARGDEESRY
                     MVKVMPQVNSMEERIRSIEKLRPQLGKVRSILGVPWMKSVRRLDEHGVTRMLADKLVS
                     AGATYAEAESAVQAAADQLWRLEKMAFVRMIRGEGFNTLWTAKP"
     gene            complement(1662..2042)
                     /locus_tag="VFH60_08175"
     CDS             complement(1662..2042)
                     /locus_tag="VFH60_08175"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HET6313796.1"
                     /translation="MAKQYAQIVGIVLLLLGVVGLVLGDPDPLFGALNIDLVEDIFHL
                     AAGAVFVYLGFANVDNATVKMVVGVIGVVLLLVGIFGFIDPRAFGLLTHPWTIVDNII
                     HLLLGVLGIAAGFLLDRDTTVTVR"
     gene            complement(2104..2292)
                     /locus_tag="VFH60_08180"
     CDS             complement(2104..2292)
                     /locus_tag="VFH60_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HET6313797.1"
                     /translation="MPQAYCVKDRKMVEMKDPQQITLKNGRPATQGTCPECGTKVTRI
                     GGGASSSGGGNGGSGGGK"
BASE COUNT          491 a          791 c          797 g          504 t
ORIGIN      
        1 aactggtagc gggcgatccc ggctccgacg ctcccagcta cgcggccctg caaggggtgg
       61 cttcggtggg gctgcccggc tcggacaggc gagtgccccc ggtagcgccc ggcgtcgttc
      121 tgccgtcgca gtacatagac cacaacggcg cggtgtcgcg caaccacccc gcgctggttg
      181 gtgccgaggc gttgaaatcg gccgggttcg ccaacgagac tgggcacaac atccctgacg
      241 tgttctggag ctatctcaat gccgaaggcc cggtgtttgt cagcaatggc tacgtgaacg
      301 acaggctggt tgactgggtg actgccttcg gctacccggt aaccgagccg ttctggacca
      361 ctatcaaggt ggcggggcgc gaccggctga tcctgttcca ggcgttccag cggcgcatcc
      421 tcacctactc gcccgccaac gcggtaggct ggaaggtgga gatgggcaac gtgggcgctc
      481 agtactactc gtggcgctac gagacccccg ctgtgacgtg ccagcgcgtg ccggtgcgag
      541 gcttcgggag agtgtgggcc gacaaccgca acgtgcagcg cgacctgggc tgtcccctac
      601 cgtatccgcc cttcgacaag gaaattagcg tcaagaccgc cttccagccg ttcgagcatg
      661 gttcgatgct gtacgtcgag cgcaccacgt acacccagga gcgcatcatc tacgtcttct
      721 tcgatgacgg caccttccag ccgttcgagg acacctggag agaggggcag ccggtaaacg
      781 gtggcctcac ccctccacag ggccgctacg agcctgtgag gggcttcggg aaggtgtggc
      841 gggagggtac aggggcacga gtacgagaga ggcttggctg ggccaccgcc acggagaagg
      901 gcgcggacgg tgcctaccag cggttcgagc ggggcgagat gtactggtcg ggcactgtgg
      961 acaagatctg ggcgctgttc aacagcgtgc ctccgggggg ctttgccgac cccacacccg
     1021 caccgggcgg gggcctgccg ccgcttcgtt acctggtgtt cgatgacacg ttcaatccct
     1081 gagcgggcag acgcccctga aaatagagcg aaaagagggg tggcgattac agtcgccacc
     1141 cctctttgct gtcaggttga aagacggtct agggctttgc ggtccagagg gtgttgaagc
     1201 cctcgccgcg tatcatgcgc acaaaggcca tcttttcgag ccgccagagc tggtcggcag
     1261 cggcttgcac cgccgattca gcctcggcat aggtcgcgcc cgcgctcacc agcttgtcgg
     1321 ccagcatgcg cgtgacccca tgttcgtcca ggcggcgcac actcttcatc cagggcacgc
     1381 ccaggatgct gcgcactttg cctagctgag ggcggagctt ctcgatggag cggatccgct
     1441 cctccatgga gttaacctgg ggcattacct tgaccatata tcgcgactct tcgtcgccac
     1501 gggcgtcgat gaccatcgcg cgtcggagga gggggaaaaa tatggtgact acttcagcct
     1561 ccgagatacc ggaaagtacc tggctcagaa actggtcttc gttgtccatt attccgcctc
     1621 catagtcctg tatcgtggtg gtgtgttgag cgtcgcaggt actatcttac agtgacggtg
     1681 gtgtctctgt ccagtaagaa gcctgccgcg atgcccagga cgccgaggag caggtggatg
     1741 atgttatcca caatggtcca cgggtgggtg agcagaccga aggcccgggg gtcgatgaag
     1801 ccaaagatgc ccaccaggag cagcacgacg ccgatcacgc ccacgaccat cttcacggtg
     1861 gcgttgtcga cgttcgcgaa gccgaggtag acgaagaccg cgccagccgc gaggtggaag
     1921 atgtcttcaa ctaggtcgat gttgagcgcg ccgaacaacg gatctggatc gccgagtact
     1981 agacctacca cgcccagtag gagtaagacg atgccgacaa tctgagcata ctgtttagcc
     2041 atctgattgt tctcccttcc gcacgccacg ccctcccgtg aagcgattgg ctacctctgc
     2101 tgcctacttg ccgcctccgc tgccgccgtt acccccgccc gagctgctgg ctccaccgcc
     2161 gatgcgggtc actttcgtgc cgcactcagg gcacgtaccc tgcgtcgcgg gccgcccgtt
     2221 cttgagggta atctgctggg ggtctttcat ctcaaccatt ttccggtctt tcacacaata
     2281 tgcctgtggc attagaacac ctccatcagt agttggttga acttgcaggc tggcccagaa
     2341 gcgaacttgc actgcagcgt gcgtgggccg tgcctgtgat gagcgtgatt atacaacgct
     2401 ccacgcgtgg agtcaataac tgttcatatc tttcgtcgca tgagggcaaa ctacctccac
     2461 gcacgtaatc ctgcagcggt tttccaccgt ttgcgccgca acctgaccgg cgcacggccc
     2521 caacacaact tgcaaacacg ggattaacta gttggaaaat aagttaatta gctaatacca
     2581 agg
//
LOCUS       DASRWZ010000526         5713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_591462, whole genome shotgun sequence.
ACCESSION   DASRWZ010000526 DASRWZ010000000
VERSION     DASRWZ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591462"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..2981
                     /gene="glgB"
                     /locus_tag="VFH60_08185"
     CDS             282..2981
                     /gene="glgB"
                     /locus_tag="VFH60_08185"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET6313798.1"
                     /translation="MPKLSQELIDSIVRGDNADPFAVLGPHHTSDDDQPSTAVRAYLP
                     WAQALQVVKHGGETHEATRIDPAGLFEAVIPGGQPFDYTLRATNEAGGTVDLHDPYSF
                     GPCLSDFDLHLIREGNHFRTYEKLGAHLLDVNGVGGVHFAVWAPNALRVSVVGDFSRW
                     DHTMYPMRQHPGAGIWEIFIPGLAEGEAYKFHVRSRYNNYEVQKSDPYGFYAEQRPRT
                     ASKVFDIGNYEWGDAEWMQERARHNALDAPISIYEVHLGGWRRKIEEQNRVFTYRELA
                     HELVDYVKMLGYTHIELMPLSEHPFDGSWGYQTIGYYAVTSRYGTPTDFMYFVDVCHK
                     NGIGVFLDWVPAHFPKDEHGLNYFDGTHLYEHADPRLGEHPDWGTLVFNFGRNEVRNF
                     LLSNALFWLDKYHIDGLRVDAVASMLYLDYSRQPGQWVPNKYGGRENLEAIDFLKRFN
                     ELVHMEYPTALTMAEESTDWPMVTKPTFLGGLGFDLKWNMGWMNDMLRYMEQDPVYRR
                     YHHNLITFSLMYAFTEQFLLPLSHDEVVHLKKSLLLKMPGDDWQKFANLRTLFGYMFT
                     HPGKKLVFMGGEFGQRTEFNEQRSLDWHELGNELNGRMLVFMRDLLYAYTRETAVHPL
                     DFRWEGFQWLNANDSDNSIISFVRRSKAPDDMVMVVCNFTPMPRYNYRVPAPRPGYYR
                     EVLNSDSSAYGGSNMGNAGGLDTVEDPWSEGGCALYLTVPPLSVLMLKPDPLPQVEAV
                     QEAVTPASEAATEAESPQANAPVAAMANATLEPALRDLNIEIGEAEKRKDLDYLGRVL
                     ADDLTFIDEQGNMFDKQQYLDGIRSPAHRTEMVASEVMGVNVRGGTADVELEVTVQGT
                     RDEVALNGTFRHARTFIRREGRWQITNWVVTKIADPMPGGSTT"
     gene            3067..3600
                     /locus_tag="VFH60_08190"
     CDS             3067..3600
                     /locus_tag="VFH60_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6313799.1"
                     /translation="MTPGTSDFFRRQVEANTEALVWAVGEVGETRLRVAPPVLLGEWP
                     AARHLFHLLYYEREVALPSLRLWFGAPYPDFEGYDEPAAWAGAPQAGAILAQIEGLRA
                     EQLDIIARADDALWAEVRQTPWAERTLYWVASKTLQHALEHTNNLLKIALLWEHYETR
                     SRRKALQAPKEGGNEIE"
     gene            3881..4297
                     /locus_tag="VFH60_08195"
     CDS             3881..4297
                     /locus_tag="VFH60_08195"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6313800.1"
                     /translation="MKEGKLQPGTDNADDSKELERINEDIGEGERDKNAEKIRDYLSD
                     DLIFLRASGKVASKQDYLDGVALPENENKQLSWDAPVITIRNDHALVVVNVTLVGMRG
                     GNHVDGVFRNFRTFMKRKNGWRLVTWANYQIVDNLA"
     gene            complement(4319..>5713)
                     /locus_tag="VFH60_08200"
     CDS             complement(4319..>5713)
                     /locus_tag="VFH60_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET6313801.1"
                     /translation="LPHRPRKEGDRHVKGIPGLPGFVGLARSRAAADLAVTAGTRGLS
                     LLLALVGNVASARALGPSDFGRFGIIMATVMICGTLADMGLTQAALRYIAREREQSDP
                     HDAARVYLVLRPLSGAIVTLLGLLLSAPLADIVLGHAEIAPYLQVAFLTLLALSISSY
                     PGTVLVGLGLFGRLGVAAMLNAVITVGGILALWLAGQLDLGTLVAWNVVVPLASSVPA
                     WLLLPKEWLPWRLAPGGAPSLWLLLQRGGLARTMLGFGKWMGLATLGSIVVSQADVLL
                     LGRLSTPETVGVYSVALALAMRLDALNQSLITVMLPRANRLRGRADMRGYSRRVLGGS
                     LLLALALGASVFLAQPLIELLYGESYRASAGLFVLLLGVVLFDLATSSLFLVALPLEK
                     PQILAVAEWLRVAALVVAGWLLIPLYAGWGAAISRMLSRVVGATYNLLALRRALNAAP
                     TTQSAGQPDNSLDA"
BASE COUNT         1159 a         1827 c         1784 g          943 t
ORIGIN      
        1 gaaggggcgc gggcggcgcg cggacaatag catcacagag cgactccagg ggtagctttg
       61 gtttcggtcc caacattaga gcgcaggggg gcggtgacgc aaagcaagtc gcaatggcgc
      121 gctgcggggg cgaggtccct acctgcggtc ttcccctctt cacacacgtt ggccctactt
      181 atgctgaaga taaccctgtc gcaagccttc actgccccgc ccagacccct atctcccacg
      241 ggcaaatacg ttacgcttgc gaccgaccgc aaggaggccc catgcccaag ctttcgcagg
      301 aactaatcga ctcgattgtg cgtggcgaca acgcggaccc cttcgctgta ctggggccgc
      361 accacacgag cgacgacgat cagccgagca ccgccgtgcg cgcctacctg ccctgggcac
      421 aagccctgca ggtcgtcaag catggtggcg agacccacga agctacgcgc attgacccgg
      481 caggcttgtt cgaggccgtc atcccaggcg gccagccgtt cgactacacc ctaagggcta
      541 ccaacgaggc gggcggcacg gtcgatctgc acgaccccta ctccttcggc ccctgcctca
      601 gcgatttcga cctgcacctc atacgcgagg gcaaccactt ccgcacttac gagaaactcg
      661 gcgctcacct gcttgacgtc aacggggtgg gcggagtgca tttcgccgtg tgggcaccca
      721 acgccctgcg cgtcagcgtg gtgggcgact tcagccgctg ggatcacacc atgtacccta
      781 tgcgccagca cccaggcgcg ggcatctggg agattttcat ccctgggctg gcagagggtg
      841 aagcgtataa gttccacgtc cgctcgcgct acaacaacta cgaagtgcag aagtccgacc
      901 cctacggctt ctacgccgag cagcgcccgc gcaccgcctc gaaggtgttc gatatcggca
      961 attacgaatg gggcgacgct gagtggatgc aggagcgcgc ccggcacaac gccctcgatg
     1021 cgcccatttc tatctacgag gtgcatttgg gcgggtggcg gcgcaagatc gaggagcaaa
     1081 accgcgtatt cacctaccgg gagctggctc acgagctggt ggactacgtc aagatgctcg
     1141 gctacacgca catagagctt atgccgctct cggagcaccc gttcgacggc tcctggggct
     1201 accagacgat tggctattac gccgtcacca gccgctacgg tacgcccacc gacttcatgt
     1261 acttcgtgga cgtgtgtcac aagaacggga taggggtgtt cctcgactgg gtgcccgccc
     1321 acttccccaa ggacgagcac ggcctcaact acttcgacgg cacccacctg tacgagcacg
     1381 ccgacccgcg cctgggagag caccccgact ggggtacgct ggtgttcaac ttcgggcgca
     1441 acgaggtgcg caacttcctc ctctcgaacg ccctcttctg gctcgataag taccacatag
     1501 acggcctgcg ggtggacgcg gtggcttcga tgctctacct cgactactcg cggcagccgg
     1561 gccagtgggt gcccaacaag tacggcgggc gggagaacct ggaagcgata gacttcctca
     1621 agcggttcaa cgagcttgtg cacatggagt accccaccgc cctcacgatg gccgaggagt
     1681 ccaccgactg gccgatggtc accaagccga ccttcctggg gggcctcggc ttcgacctga
     1741 agtggaacat gggttggatg aacgacatgc tgcggtacat ggagcaggac ccggtatacc
     1801 ggcgctacca ccacaacctg attaccttct ccctcatgta cgccttcacc gagcagttcc
     1861 tcctgcccct ctcgcatgac gaggtggtgc acctgaagaa gtcgctcctc ctcaagatgc
     1921 cgggcgacga ctggcagaag ttcgccaacc tgcgcaccct gttcgggtat atgttcaccc
     1981 accccggcaa gaagcttgtg ttcatggggg gcgagttcgg ccagcgcacc gagttcaacg
     2041 agcagcgcag cctcgattgg cacgagcttg gcaacgaact gaacggccgt atgctcgtct
     2101 ttatgcgcga cctgctgtac gcctacacgc gcgagactgc cgtgcacccg ctggattttc
     2161 gctgggaggg cttccagtgg ctgaacgcca acgacagcga taacagcatc atctccttcg
     2221 tgcggcgcag caaggccccc gacgatatgg tcatggtggt gtgcaacttc acccccatgc
     2281 cccgctacaa ctaccgggtg cccgcccccc gccccggcta ctaccgcgag gtcctcaaca
     2341 gcgattcgag tgcgtacggg ggcagcaaca tgggcaacgc ggggggcctc gacacggtgg
     2401 aagacccctg gtcggagggt ggctgcgccc tctacctgac cgtgccgcct ctctccgtac
     2461 tgatgctcaa gcccgacccg ctaccccagg tggaagcggt gcaggaggct gtgacacccg
     2521 cgtcagaagc ggccaccgag gccgagtctc cccaggctaa cgctcccgtg gccgcgatgg
     2581 ctaacgccac gctcgagcct gcccttcgcg acctcaacat cgagattggc gaggccgaga
     2641 agcgaaagga cctcgactac ctgggccggg tactggcgga tgacctcacc ttcatcgacg
     2701 agcaaggtaa tatgttcgac aagcagcagt acctggacgg cataaggtct cccgcgcaca
     2761 ggaccgaaat ggtcgcctcg gaagtgatgg gggtgaacgt gcggggaggt accgccgacg
     2821 ttgagttgga agtcacggtg cagggcacgc gggatgaggt agccttgaac ggcacgttcc
     2881 gccacgcacg gactttcatc aggcgagagg gccgctggca gatcaccaac tgggtagtca
     2941 cgaagatagc ggaccctatg cctggtggct ccacgactta gaacctgcgt ggctggagac
     3001 gcgagcgccc ggcatgtaat tcaggcggcc gcacgggcgg acaccacact tccaggaggc
     3061 agtcttgtga cacctggcac gtccgacttc ttcaggaggc aagtcgaggc taacacggag
     3121 gcgttggtgt gggctgtggg ggaggttggg gagacgcggt tgcgtgtcgc gccccctgtg
     3181 ctgctgggcg aatggccagc ggcgcgtcac ctgttccacc tgctctacta cgagcgcgag
     3241 gtggccctgc cgagcctgcg tctttggttc ggcgcacctt accccgactt cgagggctac
     3301 gatgaaccgg cagcctgggc gggcgcaccc caggccgggg cgatccttgc ccaaatcgag
     3361 ggtctgcggg ccgaacagtt ggacatcata gcaagggcgg acgatgcgct ctgggccgag
     3421 gtgcggcaga ccccctgggc cgagcgcacg ctctactggg tggccagcaa aacccttcag
     3481 cacgcactag agcacaccaa caacttactc aagattgccc tgctgtggga gcactatgaa
     3541 actcgctcac gacggaaagc tctgcaagcc cccaaggagg gcggcaacga gatagagtga
     3601 ggctccatat agcagttgga cctgcgtggg cctcctgcaa agtttgcgtg gtgtgctaat
     3661 ggagttccgg ggaatagcaa ctgcgacttc atgcctgtag tcggcgagtc cgagcgccgg
     3721 acaatggagt tcgcgagcgg actctagaaa atacggcgac ttgcggataa aatagcggca
     3781 cggcgatcac atggtttgga tgggacctcg tacagccgtt cctcacagcc tgtgtgccgt
     3841 gacatcgtat gaggtggtcg tccacatatt gagccatagt gtgaaggagg gcaagttgca
     3901 gccagggact gacaatgctg atgacagcaa agagttggag aggatcaatg aggacatagg
     3961 cgagggcgag cgtgacaaaa acgcggagaa gattagggac tacctgtccg acgacttgat
     4021 attcctcaga gcgagcggca aagtagccag caagcaagat tacctggatg gtgtggctct
     4081 acctgagaac gagaacaaac aactctcctg ggatgctcct gtgatcacga taaggaacga
     4141 ccatgccctg gtcgtggtca acgtgacgct ggtaggcatg agaggaggca accacgtaga
     4201 cggtgtgttc cgcaacttca ggacgttcat gaagcgtaaa aacggttggc gtcttgtgac
     4261 atgggctaat taccaaattg tagacaacct ggcttagccc gtggtaatcg gcgctctttc
     4321 acgcgtcgag ggagttatca ggttggcccg cgctctgcgt ggttggagcg gcattaagtg
     4381 cgcggcgcaa cgcgaggagg ttatacgtgg ctcccacgac ccgcgacagc atgcgcgaga
     4441 tggccgctcc ccatccggcg tagaggggta tcagcagcca acccgctacc acgagggcag
     4501 ctacgcgcag ccactccgcc accgccagta tctgcggctt ttcgaggggt agcgccacca
     4561 ggaagagcga gcttgtcgcg aggtcgaaca gcaccacccc caacaacagc acgaacagcc
     4621 cggcggacgc cctgtagctc tccccataca gtagctctat aagcggctgc gccaggaaaa
     4681 cggaggcccc cagcgccagc gccagcaaga gcgacccgcc cagcacccgg cgcgagtacc
     4741 ccctcatgtc ggcccgccca cgcagccggt tcgccctggg tagcatcacc gtgatgaggc
     4801 tctggttgag cgcgtccagc cgcatagcca gcgccagcgc gaccgagtag acacccaccg
     4861 tctcaggggt cgatagcctc cccaggagca gcacgtcggc ctgggagacc acaatcgagc
     4921 cgagggtggc gaggcccatc cacttgccga agcccagcat cgtccgggca agcccgcccc
     4981 gttgcaggag cagccacagc gagggcgcac cccctggtgc cagtcgccag gggagccatt
     5041 ctttgggcaa gagcagccag gccggtacgc tgctggcgag gggcaccacc acgttccagg
     5101 caaccagggt acccagatcg agttgccccg cgagccacag cgccaggatg ccgcccaccg
     5161 taatcaccgc attgagcatc gccgccacgc ccaggcgccc gaacagcccc agccccacga
     5221 gcacggtgcc cggatagctg cttatcgaca gcgccagcag cgtcaggaaa gccacttgca
     5281 ggtagggcgc tatctccgca tggcccagca ctatgtccgc caggggcgcg gagaggagca
     5341 ggcccagcag ggtcacgatg gcccccgaca gcgggcgcaa cacgaggtac accctggcgg
     5401 cgtcgtgcgg gtcgctctgt tcgcgctccc tggcgatgta ccggagggcg gcctgggtga
     5461 ggcccatatc ggccagggtg ccgcatatca tcacggtagc catgataatg ccgaacctgc
     5521 cgaagtcgga ggggccgagg gcgcgggccg aggcgacatt tcccaccagc gcgagcagca
     5581 gcgacaggcc acgagtgccc gcggtaaccg ccaggtctgc cgccgccctg ctgcgcgcta
     5641 accctacgaa accaggcagt ccggggatgc ccttcacgtg tctatcgcct tccttccgag
     5701 ggcggtgggg tag
//
LOCUS       DASRWZ010000527         9769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1344505, whole genome shotgun sequence.
ACCESSION   DASRWZ010000527 DASRWZ010000000
VERSION     DASRWZ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344505"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1361)
                     /locus_tag="VFH60_08205"
     CDS             complement(195..1361)
                     /locus_tag="VFH60_08205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6313802.1"
                     /translation="MLTITLPFSVEALQGALSRYLSRPTLSIEEIIPERLGGGVSGSP
                     VYRLTVNYRYGSDSTSPSLPQPSSSDVLRLVLKRGTRHTGAILAGSARREASFYRSLA
                     GQLPVHTPRALLTADDVTGEPTVPLVVDAPGNLAQGWDGAFDSDWVLMEALPSDVVWP
                     QSTWTAEHYRSALTALAGLHARWWGCPPSASDYPWVWTPTGQHTEDLVREARESMLEI
                     AGQPWGEKFFPGQRLQAWLDVLDNPACLLDTLTGMPQTLIHGDYWPGNIAMRADGPAV
                     FDWQLVGVGPAPYDLACFHSSSRWWFGRLPLSLTEMRSYYLARLNEQLGYSVDRYSFD
                     TGMDAARAWRFAVLWPTPILEHHGSLLAQLHHMRATAIEPAFASLRRCLGYIPH"
     gene            complement(1954..2847)
                     /locus_tag="VFH60_08210"
     CDS             complement(1954..2847)
                     /locus_tag="VFH60_08210"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HET6313803.1"
                     /translation="MLDTAPLGAIDAGPEVPRPTREDDGPMPFNDQVIERSLTRLRLS
                     GPELSHSRANNVDAARRLADNDPYVTFGIRLVIDYVQLNHVEPQEALAMIASITKCSQ
                     DVSYTEGRGYISPASTIKGLKAMSAAIRRVSREHGYVAFATGHPGSMIECYTELAGLV
                     RELGGHVLDLAKGQLVFQGEDRDHVHVADSVNGIAILSDTCAALHTHDSRAMQLMLDD
                     ARRLPDLVIADHGFAGEAINRGIPTVAVMDTNAPGLAVAKKLGANVVIVPMNDNRPNM
                     VTRKAAELVRQMVLESDRARR"
     gene            3157..5325
                     /locus_tag="VFH60_08215"
     CDS             3157..5325
                     /locus_tag="VFH60_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6313804.1"
                     /translation="MKVIPTTGPDVELYPARTPSAQTVKVLLVEANPVLAEAVLGHLA
                     ATRRVRADVTNAPCLADALAYLSAEQFHTILLDLDLPDGSPPQICEAVREDCGAAVII
                     VLADSDEDLTSVEALRRGADDYLVKSRINADLLDRTIGYTLQRRRDIEEQVVAYQMLE
                     QRVADRTRELSTMLEVSQCVLSTLQLKPLISLILDQLKTLVDYTSATFFTFDGERFEG
                     IDYRGPIPIEVVRQIPSPLKAAGHYMEVIRRGEPIIVGDLTGDHPLAIAFKETAGELA
                     RTYYSYIRSWMGVPLMVKDRPIGVLSMAYDEPDRYTEHDARMAMAMSNQAAIAIENAK
                     LYEEAQEAVRRTTALAQVASSLAFEGSLETILDGLACNVVQATGAEACAVLLLNEKTG
                     DTVYAGAYGLPEGYAAANQAARQSLPDIEPISHKVLRLQEPFVRRNVRQWVLGTPEME
                     PLHVFMRDIAWDTIIGVPLNYRGRPLGALCSFCREDSASVEEEIAFLSAIADQAAVAV
                     ENARLFSEVQGKAALEERQRLARELHDSVSQAIFSISLHARTAEALLDRGDSARIGET
                     VEHISSLAQAAMAEMRALIFELRPESLAAEGLVAALTKQAAALRARHGIQVCTELCRE
                     PDLPIETKEVVYRVAQEASHNTIKHAHAGTLGLRLECDDANITLVVEDDGQGFDPGMQ
                     FPGHLGLQSMRERAQRLGGSLTIASAPGSGTAVRLRLPVS"
     gene            5328..6149
                     /gene="nudC"
                     /locus_tag="VFH60_08220"
     CDS             5328..6149
                     /gene="nudC"
                     /locus_tag="VFH60_08220"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HET6313805.1"
                     /translation="MDAVPRFVPGVSPPEESAQPCRWFAFSGPRLLVLEEGGTTTVPI
                     ALDPTELGVEVVRRQYLGSMEGQGCYAAELAPDFRPPEGMSLQGLRGLYGRLDEVCYA
                     VAGRAFQIVEWDRTHLYCGHCGTPTEQVAGERAKRCPACGLTGYPRVTPAIIVLITRG
                     DEVLLAHAHNFPSAFYSTIAGFVEPGESLEEAVAREVGEEVGVAVKDIRYFGSQPWPF
                     PHSIMIGFTCEYAGGEIRLEEREIADAAWFTRDNMPHIPPKLSIARKLIDSFLRG"
     gene            complement(6139..7491)
                     /locus_tag="VFH60_08225"
     CDS             complement(6139..7491)
                     /locus_tag="VFH60_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HET6313806.1"
                     /translation="MSKEMNQRSIEERVADTLHERATRDVPYNLNLWPQISSRAMKQR
                     HTYRGMSRLPFRRPNLVVAAYLAILVVAVALTFSLAPTLKSDGSTAVSSGGGQQQPSD
                     IAVAATQEASGQHTPLPSITPDATATQYTADYNAAKAPYAYLDRSKYGKHLGISQTSG
                     DYTVTINWMYADGNRLLIDFDLLGPQPDPGSDRRFTPYVAALSVKDGPTFSAVENRSS
                     SRVEGNRAHYLVEKDAAFILDKYGNQELEFNLTIAVDAINITPKAGGTLPDATATWIA
                     DSQDVERIRERDIAGPFNFQLSVPFIPARTAGLLQTASANNVEMALQQVRVSPAEIRS
                     YILVASGDKTLSPDEWQISAGAQVNDWDALMFRTGKTPFGRMVTSTVGEHTIYDNVYD
                     QEGEWTFTVRQLLNSRTQETIQGPWVFKFEFPNAGTNTGRWRLAEQSPVPYRGPGTSP
                     "
     gene            complement(7525..8124)
                     /locus_tag="VFH60_08230"
     CDS             complement(7525..8124)
                     /locus_tag="VFH60_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6313807.1"
                     /translation="MREAEAIARLKRGDISGLEGLVAVYQVQAVRAAYLVTRDLGLAE
                     DVVQSAFLRAYERIGQYDTARPFAPWFLRSVINDAIKVSTRGRHVSLDALMPLEGTLL
                     KSYSEALDLGSLIVEAETNDAIWEALGKLPPQQRAVLVLRYYLDLGEAEMSARLDVPA
                     GTVKSRLHAARKHLRALLPSWLTPPSAGEENDNKHASYL"
     gene            8454..>9769
                     /locus_tag="VFH60_08235"
     CDS             8454..>9769
                     /locus_tag="VFH60_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313808.1"
                     /translation="MFSLKRAVAARDTRVGPDTAIKIAEQGRYNFKIGVANGVLAGLL
                     DPLSGIFVLTLFLARLDAPAFLVGLLPTIASCGGLLPQLIVAGKLRGKKRVMPWYKWA
                     SVVRVAGLAGMAVSAWLIPLDRDLALVSLFASYLVYMLSSGVSGLPFMEVMGKIVPPH
                     KKGTFFGLRQFGAALLGIVTSAIAGAVMSEHLGLTFPYNFAAMLSVATVAGAVVFGMW
                     ALVREPDLDVAQMSRPETGSIFRHGATALQTNRDYRLLTVVRLLATFSGIAGPFYLVF
                     AAKALEVPVGVLALYMTVQTAIPLASGVLWMPLANKASRRTLVLLTAALQLAVPVTAL
                     IVGGIASAGVLPVSWNAWLAASIFIVGSLAGSSAVMFNDLVLLSIAPNGERPVYFGFL
                     NTLAGVASLTLPLGGLLLEYMGYTSLFALSGILALFSLLAGLGIERP"
BASE COUNT         1632 a         3028 c         3245 g         1864 t
ORIGIN      
        1 ctcaccgacg cgcggccttc tctggtcgct cgtgggggtg gttgcggcga gtgcctgggg
       61 agtgtaggca ggctcgtact ccgcgaatgc ggcaaccagc acggcggcga ttgccggggc
      121 ttcctccggg acggcggtgc ggacctgtat gctgggcgta tgaggtgcca tattcgacct
      181 gagcacactc cggcctagtg aggtatgtac cccaggcacc ttctgagtga ggcgaaggca
      241 ggctcgatgg cggtggcgcg catgtggtgg agttgggcga gcaggctgcc gtggtgctcc
      301 agtatggggg tgggccagag cacggcgaag cgccaggcgc gggcggcgtc catgccggtg
      361 tcgaacgagt agcggtccac gctgtagccg agttgctcgt ttaggcgggc caggtagtag
      421 ctgcgcatct cggtgaggga gaggggcagg cggccgaacc accagcggga tgaggagtgg
      481 aagcaggcga ggtcgtaggg agcggggccg acgcccacaa gctgccagtc gaacacggcg
      541 gggccgtcgg cgcgcatagc gatgttgccg ggccagtagt cgccgtggat gagggtctgg
      601 ggcatgccgg tcagcgtgtc gagcaagcac gcggggttgt cgagcacatc gagccaggcc
      661 tggagccttt ggccggggaa gaacttctcg ccccagggct gccccgcgat ctccagcatc
      721 gactcccgcg cctcgcgtac gaggtcctcg gtgtgctggc cggtgggcgt ccacacccac
      781 gggtagtcgc tggcgcttgg cgggcagccc caccagcggg cgtgcaggcc ggccagggcg
      841 gtgagggcgc tacggtagtg ctcggcggtc caggtggact gcggccacac cacgtcggag
      901 ggcagcgcct ccatcagcac ccaatcgctg tcgaacgcac catcccagcc ctgggcgagg
      961 ttgccgggcg cgtccactac cagcggcacg gtcggctcgc ccgtcacgtc gtcggcggtg
     1021 agcagggcgc ggggcgtgtg cacgggtagc tgcccggcga ggctgcggta gaaggaagcc
     1081 tcccgccgcg ccgaccccgc cagtatagcc cccgtgtgcc gggtaccgcg cttgagcact
     1141 aatcgaagca cgtcgctgga cgagggttga gggaggctag gtgaagtgga gtcgctgccg
     1201 tacctgtagt tcacggtgag gcgataaacg gggctgccgg agacgccgcc gcccaggcgt
     1261 tcgggtatga tctcttcgat gctgagtgtg ggacgtgata ggtagcgcga cagtgcgccc
     1321 tgtagagcct cgacgctgaa aggcaaagta atagtgagca ccgcacgcct ccgatccatg
     1381 aatggcaaac aggttgagcc aggcaccggg cacaagcgcc caacgcctca gatgcagaag
     1441 agacctgggc ataacgcgct tgaacagcaa agcagcgcgc ttacgcaggt tgaaccgaag
     1501 aaaaaaagtg tgacgacagc acgccccacg cgtaacgcgg gagcatggac catctttgtc
     1561 ttgtctaaag gattatattt acgcgccggt aggggatacc ggccctcagc cgcgtcccac
     1621 gcgacaataa gtagctaatt ttaccagcgg gttaggcggc tgtcaaggct aaaatgaggg
     1681 tacttttggc gcgtccggat gaccaggatg gctacctgga aaatacacaa agaaagacga
     1741 aaccgcgaag gcgcaaagag cgcaaagggc gcataaaggc taactattgt ggctcaaata
     1801 gaggaccagg agagtacctg gcgtggtacc ctcctggtcc tctttgtact ctctaaacca
     1861 cagctacttt tcacgcgtcc tttgcgctct ttgcgccttt gcggtttcgc cttctattct
     1921 gttgctattt gtgaggttgc cggatatacg ccgctatcgc cgcgccctat cgctctccag
     1981 taccatctgc ctgacgagtt cggcggcttt gcgcgtaacc atgttggggc ggttgtcgtt
     2041 catagggacg attacgacgt tggcccccag cttcttggcg acggccaggc cgggggcgtt
     2101 ggtatccatc acagccaccg tggggatacc ccggttgata gcctcgcccg cgaagccgtg
     2161 gtcggctatc accaggtcgg gcagcctgcg ggcgtcgtcg agcatcagtt gcatggcgcg
     2221 ggagtcgtgg gtgtggaggg ccgcacaggt gtcggagagg atggcgatac cgttcaccga
     2281 gtcggctacg tgcacgtggt cccggtcctc gccctggaag acgagctgcc ccttcgcgag
     2341 gtcgagcacg tgcccgccaa gctctcgcac cagcccggca agctcggtat agcactctat
     2401 catcgagccg gggtggccgg tggcgaacgc cacgtagccg tgctcgcggg acacgcgccg
     2461 gatggcggcg gacattgcct tcaacccctt gatcgtggag gcaggggaga tgtagccgcg
     2521 cccttcggtg tagctcacgt cttgcgagca cttggtgatg ctcgctatca tcgcaagggc
     2581 ctcctgcggc tcgacgtggt tgagctgcac gtagtcgatg accaggcgga tgccgaacgt
     2641 cacgtagggg tcgttgtccg cgagccgcct ggcggcatcc acgttgttgg cgcgcgagtg
     2701 gctcaattca gggccggaca gccggagcct ggtcaggctg cgctcgatca cctggtcgtt
     2761 gaagggcatc ggtccgtcgt cctctcgtgt ggggcgtggg acctccgggc cggcatctat
     2821 cgcgccgagg ggtgcggtat ctaacatggc gcgattctag acaaaggtgc cgccgcggag
     2881 gtgcttatac acattcccct gcttctattc tgagtctact ctattgcaag attccggcca
     2941 gaaggtttgc taacagggct aaaatctgat acgatatgac tatcggacag cgttacacac
     3001 gacgctgccg cacgcccggt acactgcagc tatgagctat acacggggcg gctatcacgt
     3061 tgtggtgctg attggtcctg gcatattgga gggcggtgcg cttttatagc cgccctgcct
     3121 ctctgtagcg tcctaaccgg ctagatgaag cagtagatga aagttatccc tacaaccggc
     3181 cccgacgtgg aattgtaccc cgcccgcacg ccctcagcgc aaacggtaaa ggtgctgctg
     3241 gtggaggcca accctgtgct tgccgaggca gtgctggggc acctggccgc cacccggcgc
     3301 gtgcgggccg acgtgaccaa cgccccgtgc ctggcggacg cgctcgcata cctgagtgcc
     3361 gagcagttcc acactatctt gctggacctg gacctgcccg atggctcccc gccccaaatc
     3421 tgcgaggcgg tgcgggagga ttgcggcgcg gcggtcatca tcgtgctggc cgacagcgac
     3481 gaagacctca cgtcggtgga agcgcttcgc cggggcgcgg acgactacct ggtgaagtcc
     3541 cgcatcaacg ccgacctgct cgacaggacg ataggctaca cgttgcagcg caggcgcgat
     3601 atcgaggagc aggtggtcgc ctaccagatg ctggagcagc gggtggcgga ccgcacacgc
     3661 gagctatcca cgatgcttga ggtgtcgcag tgcgtgctct ccactctgca actcaagcca
     3721 ctcatcagcc tcatcctgga ccagctaaag acgctggtag actacacgag cgccaccttc
     3781 ttcaccttcg atggcgagcg gttcgagggc atagactatc gaggccccat tcccatcgag
     3841 gtggtccggc agattcccag cccgctcaag gcagccgggc actatatgga agtcatccgg
     3901 cgcggggagc ctatcatcgt gggggacctc acgggcgacc acccgctagc gatagccttc
     3961 aaggagaccg ccggagagct tgcccgtacc tactacagct acatccgctc ctggatgggc
     4021 gtgcccctca tggtgaaaga ccggccgatt ggcgtgctga gcatggccta cgacgagccg
     4081 gaccgctata ccgagcacga cgcccgcatg gctatggcga tgtccaacca ggccgctata
     4141 gctatcgaga acgccaagct gtacgaggag gcgcaggagg cggtccgccg gacgaccgcg
     4201 ctggcgcagg tagcctctag cctggccttc gaaggctccc tcgagacgat cctggacggc
     4261 ctggcgtgca acgtggtgca ggccaccggg gccgaggcgt gcgcggtgtt gttgctcaac
     4321 gagaaaacgg gcgatacggt ttacgcgggc gcttacggcc tgccggaagg ctacgccgcc
     4381 gccaaccagg cggcccggca atcgctcccc gacatagagc ctatttccca caaggtgctg
     4441 cgcctgcaag agcctttcgt gcggcgcaac gtgcgccagt gggtgctcgg cacgcctgag
     4501 atggagccgc tccacgtgtt catgcgcgac atcgcgtggg acactattat cggggtgcct
     4561 ctcaactaca ggggcaggcc gctaggcgcg ctgtgcagct tctgccgcga ggactccgca
     4621 tccgtggagg aagagatagc cttcctgtcg gccatagcgg accaggcggc ggtggccgtg
     4681 gagaacgccc ggctcttttc cgaggtgcag ggcaaagcgg ctctggagga gcggcagcgg
     4741 ctggcgcggg aactgcacga ctcggtgtcg caggcgatat tcagcatctc cctgcacgcc
     4801 cgcaccgccg aggccctgct ggaccggggc gactccgcgc gcatcgggga gacggtggag
     4861 cacatatcgt cgcttgccca ggccgcgatg gccgagatgc gcgccctcat attcgagctg
     4921 cggcccgagt cgctggctgc cgaggggctg gtggccgccc tcaccaagca ggccgccgcc
     4981 ctccgcgcgc gccacggcat ccaggtgtgc accgagcttt gccgcgaacc cgacctgccg
     5041 atagagacca aagaagtggt gtaccgggtg gcgcaggagg cctcgcacaa cacgatcaag
     5101 catgcccacg cgggcacact gggcttgagg ctggagtgcg acgacgcgaa tatcaccctg
     5161 gtggtggagg acgacgggca gggcttcgac ccaggcatgc agttccccgg ccacctgggg
     5221 ttgcaatcga tgcgcgagag ggcgcaacgg ctgggcggca gcctcaccat cgcgagcgcc
     5281 cccggcagcg gcaccgccgt gcgcctgcgc ctgcccgtca gctaggtatg gatgccgttc
     5341 cccggtttgt gcccggcgtg tctcccccgg aggagagtgc gcagccttgc cggtggttcg
     5401 ctttctccgg cccccgcttg ctggtgctgg aggagggcgg cacaaccact gtgcccatcg
     5461 ccctggaccc tacggagcta ggggtggagg tggtgcggcg gcagtacctc ggcagcatgg
     5521 aggggcaggg ctgctatgcc gccgagcttg cgcccgattt caggccgccg gaagggatgt
     5581 cgctccaggg tttgcggggg ctgtacggcc gcctcgatga ggtgtgctac gcggtcgcgg
     5641 ggcgggcctt tcagatcgtg gagtgggacc gcacccacct ctattgcggg cactgcggca
     5701 cgcccacaga gcaggtggcg ggcgagcggg ccaagcgctg ccccgcctgc gggcttaccg
     5761 gctatccacg cgtcacaccc gccattatcg tgctcatcac gcggggcgac gaggtgctgc
     5821 tggcccacgc ccacaacttc ccttccgcct tctacagcac catcgcgggc tttgtcgagc
     5881 ctggagagtc gcttgaagaa gcggtggcgc gggaggtggg tgaggaggtg ggggtggcgg
     5941 tgaaggacat acgctacttc ggcagccagc cctggccctt cccccactcc atcatgatag
     6001 gcttcacctg cgagtacgcg ggcggcgaga tccgcctgga agagcgcgag atcgccgacg
     6061 ccgcctggtt cacgcgagac aacatgcccc acataccccc gaagctgagc atcgcccgca
     6121 agctgataga ctcgttcctc aggggctagt gcctggcccc ctgtaaggta ccgggctttg
     6181 ttccgctaat ctccagcgcc ctgtgttagt acctgcgttg ggaaactcga acttgaagac
     6241 ccacggcccc tgtattgtct cctgcgtacg ggagttcaga agctgccgta ccgtgaaggt
     6301 ccattcgcct tcctggtcgt aaacgttgtc gtagatcgtg tgctccccca ccgtgcttgt
     6361 gaccatccgg ccgaacgggg tcttcccggt cctgaacatg agagcgtccc agtcattgac
     6421 ctgagcgcct gcgcttattt gccactcatc agggctgaga gtcttgtccc ccgatgcgac
     6481 caggatgtag cttctaatct ctgccgggct taccctgacc tgttgcaagg ccatctcaac
     6541 gttatttgcg ctcgcggttt ggagcagccc ggcggtccgt gcggggatga acggcacgct
     6601 caactggaaa ttgaaggggc cggctatgtc acgttctctg atacgctcca cgtcctggct
     6661 gtctgcgatc caggttgcgg ttgcgtctgg taaggtgcct ccggccttcg gcgtgatatt
     6721 gatagcatcg accgcgatag tgaggttgaa ctctaactcc tggttcccat acttgtccag
     6781 tatgaacgct gcgtccttct ccaccaggta atgagctcta tttccttcca ccctgctgct
     6841 ggaccgattc tctacggctg agaaggtggg gccatctttt accgatagcg cggcgacgta
     6901 tggcgtgaag cgcctgtccg accccgggtc gggctgtggt cccagcaggt caaagtcaat
     6961 cagcagccgg ttgccatccg catacatcca gttgatagtg accgtgtagt ctccactggt
     7021 ctggctgata ccgaggtgct ttccgtattt gctcctgtcc aggtatgcat acggggcttt
     7081 ggcggcattg tagtccgccg tgtattgagt ggctgtggcg tcgggggtga ttgagggcag
     7141 gggggtgtgc tgcccgctcg cctcctgcgt ggcggcgacc gctatgtcgg agggttgttg
     7201 ctgtccgccg ccggaggaga ccgccgtgct tccatcgctc ttgagggtgg gggcgaggct
     7261 gaaagtgagg gccacggcga ccaccagtat cgccaggtaa gccgccacga ccaggtttgg
     7321 cctgcggaac ggcagccgcg acatgcccct gtacgtatgc cgttgcttca ttgcccggct
     7381 gctgatctgg ggccacaggt tgaggttgta gggtacgtcc ctggtagccc gctcgtgcag
     7441 cgtatcggcc acgcgttctt ctatgctgcg ttggttcatc tctttactca ctttctcggc
     7501 tcccgtgccc ctagtatgca ggtgtcacag gtagcttgcg tgcttgttgt cgttttcttc
     7561 tcccgcggag ggaggggtga gccacgaggg cagcagcgcc cgcaggtgct tgcgagccgc
     7621 gtgcaggcgc gatttcacgg tgcccgccgg tacgtcgagc cgggccgaca tctcggcttc
     7681 gcccaggtcg aggtagtagc gcagcacgag caccgcccgc tgttggggcg gcagcttgcc
     7741 cagcgcctcc cagatggcgt cgttagtctc ggcctccacg ataaggctgc ccaggtccag
     7801 cgcctccgag tagctcttga gcagggtgcc ttccaggggc attagcgcgt cgagggagac
     7861 gtggcggccc ctggtgctca ccttgatagc gtcgttgatg acgctccgca ggaaccaggg
     7921 cgcaaagggg cgggcggtgt cgtactggcc tatgcgctcg tacgcacgca ggaaggccga
     7981 ctgcaccacg tcttcggcga gaccgaggtc acgggtcacc aggtaggccg cgcgcaccgc
     8041 ctgcacctgg tagaccgcta ctaggccctc tagtccgctt atgtcgccgc gcttcaaccg
     8101 cgcgatggct tctgcttctc tcatacgagc gtccttaggt ccggggtgtg tggctgtatt
     8161 tggaccttca tagctactat cccggcacta tgtaaaaggt tcagcgtcgc gctgcccaat
     8221 accgctaatg agcgtgataa tacgccgaat ggcgcaacca tcccgcgcga ggagccgtta
     8281 taatttacgt aatcggtcct ctgtaccctc ccgtcatcca gcgcaggacg ccgggcagca
     8341 tcgctgcaag gggcgtgatc gcccatacgt tgcaccgttt cgcacattat cactcttaat
     8401 ctcttattta gaaccggcac ttattttgcg ctggatttct ggagcggcaa atcatgtttt
     8461 cgttgaagcg agccgtggcg gctcgcgaca cccgggttgg tcctgacacc gccatcaaaa
     8521 tagccgagca aggcaggtac aacttcaaga ttggcgtcgc gaacggcgtg ctcgcgggcc
     8581 tgctcgaccc tctctcaggc atcttcgtac tcaccctctt cctggcccgc ctcgatgcgc
     8641 ccgccttcct ggtcggcctg ctgcccacga ttgcgagctg cggcggcctg ctgccccagt
     8701 tgatcgtggc gggcaagctg cgcggcaaga agcgcgtcat gccctggtac aagtgggcct
     8761 cggtggtgcg ggtagcgggg ctggcgggca tggcggtatc ggcctggctc ataccgctgg
     8821 accgcgacct agccctagtc tctctgttcg ccagctacct ggtgtacatg ctcagctccg
     8881 gcgtgtcggg cctcccgttc atggaagtca tgggcaagat agtgccgccg cacaagaagg
     8941 gcaccttctt cggcttgcgc cagttcgggg ccgcgctgct cggcatcgtg acttcagcca
     9001 tcgcgggcgc ggtcatgtcg gagcacttgg ggctgacttt cccctacaac ttcgcggcga
     9061 tgctcagcgt ggccacggtg gcgggcgcgg tggtgttcgg catgtgggcg ctggtgcgcg
     9121 agcctgacct ggacgtggcg cagatgtcca ggcccgaaac ggggtcgatc ttccggcacg
     9181 gggcgacggc cttgcagacg aaccgggact accgcctgct gacggtggtc cgcctgctgg
     9241 ctacgttctc cggcatcgca ggcccgttct acctggtgtt cgccgcgaaa gcgctggaag
     9301 tgccggtggg cgtgctggcc ctctacatga ccgtgcagac ggccataccc ctcgcgtcgg
     9361 gcgtgctgtg gatgccactg gcgaacaagg cttcccggcg cacgctcgtg ctgctaacgg
     9421 cggccctgca actggcggtg cccgtaaccg cgctaatcgt gggtggtatt gcgagtgcgg
     9481 gtgtgctgcc ggtaagctgg aacgcctggc tcgcggcgtc gatcttcata gtgggcagcc
     9541 tggcaggctc ctcggccgtc atgttcaatg acctcgtgct gctgtccatc gcgccaaacg
     9601 gcgagcgccc cgtgtacttc ggcttcctca acaccctcgc gggggtcgcc agcctcaccc
     9661 tgccgctggg cggcctgcta ctcgaataca tgggctacac gtccctgttc gcgctctccg
     9721 gtatcctggc gctcttctcg ctgctggcgg ggctggggat agagagacc
//
LOCUS       DASRWZ010000528         4673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_454186, whole genome shotgun sequence.
ACCESSION   DASRWZ010000528 DASRWZ010000000
VERSION     DASRWZ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454186"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..1911)
                     /locus_tag="VFH60_08240"
     CDS             complement(325..1911)
                     /locus_tag="VFH60_08240"
                     /inference="COORDINATES: protein motif:HMM:NF027641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0051118 - glucan
                     endo-1,3-alpha-glucosidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,3-alpha-glucanase family
                     glycosylhydrolase"
                     /protein_id="HET6313809.1"
                     /translation="MPQHLKQASRARKPFIIKPRLGSQFRWLLILAALLTLTSGVAPA
                     SLAAPTSTIAGEKLVLAFYYMWYGPDSFSQGRTAGMPQAPYISDKADVVERQVKEAKG
                     AGIDAFISSWTGNGTETDRNFPLLLDAAARNNFRATIYFETNSVMQKGDVVSQLRSVM
                     ERFGSHPAFLRWNGKPVVFFWRPDALGDAATWRSVRQKVDPANSQLWSVDTVDGAYLD
                     AFDTIHFFSAGKWDANTDVARVQNQWRAKVDAYNKQHGTQRLWTGGVTPGWDDSKSVQ
                     PNTPIKVIPRKDGALYEENWRAAIAANPEWITITSWNEWFEGSQIEPAADYGNRYLDI
                     TRQYSALFKVGPSPCDGGTPFRETGYTLCKAMESYWRQYGGLAQFGYPISAPSVERSP
                     IDGKDYTVQYFERARFELHPEQKGTPYEVQLSLLGRQFHAVDPPVARNNQPGHKYFPE
                     TGHNVSPAFYKYWQEHGGLFVNGYPITEEIQERLSDGKTYTVQYFERVRMEFHPEHPA
                     PNNVLLGLLGRTALEQKHGR"
     gene            complement(2010..3671)
                     /locus_tag="VFH60_08245"
     CDS             complement(2010..3671)
                     /locus_tag="VFH60_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET6313810.1"
                     /translation="MLQVQHLSKSYGVNTVLAAVNLIVNDGEHVGLIGPNGSGKSTLL
                     KIITGQEPPDGGTVVLSPRGLRVGYLAQSFADVTGRTISEVLAAAQGELATAETDLQQ
                     AADALTTAADVEAAMQAYNDALTRYEALGGYEREHRSAAILQGLGLGDIPAYTRVGEL
                     SGGQKTRLGLATLLMQEPDLLLLDEPTNHLDVEALEWLEEFVGSYPNSVLIVSHDREF
                     LDRTVTRVIYLDPGTHTAKSYPGNYSDFAEAREQERLAHIEAWTRQQEYVSRVRTDIA
                     RWKGWALDIERGTTPRQPNVRRSARRKAQLAKSRETKLERYMEADERVDKPALSWWLK
                     LDFGTPPPSGRAVLHMEDVTFRYPGGPTILEGASLDVGYGERVAFVGPNGAGKSTVLK
                     LIDGTLRPDGGTLKIGSGVRLGRLSQEQETLRPDWTVLQTVLNERPMSETEARSFLHF
                     FLFEGDSVFRRVDECSLGERSRLQLAVLVLRGCNLLLLDEPLNHLDIDGREHFQQALE
                     AFEGTVVVVAHDRAFLHDYPERVVEVKDGGLRVYEGYQFYLEHKG"
     gene            complement(4246..>4673)
                     /locus_tag="VFH60_08250"
     CDS             complement(4246..>4673)
                     /locus_tag="VFH60_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6313811.1"
                     /translation="AEARKAEGAQMILALDSLGSASTWAGDYDRARKFLQAAVEVNGD
                     YLPARADLALALLASGDAKGAGSEYDRAVKSAVTYTRGTDGAALKGASLNAGFEDART
                     HLEAAALDLERLMNERPELEEAGRQLFDRLNRAASAYER"
BASE COUNT          787 a         1332 c         1575 g          979 t
ORIGIN      
        1 ggggctgttc gggcgggtga agttcaacac ggggcttctg agccgcatgc cgatggcctc
       61 cgcaatcgcg gtatcgtgcc tgggcatctt cctggctatc aactccctcg gccctaacta
      121 gcaagatatg gggttaaggc agtaataagg gacgaaaacg caaagacgca aaggacgcaa
      181 agaacgcgca gagattaagg attgttgttc taagcaagag ggccaacata cgacatctgt
      241 accctggccc tcttaccact cttcatacct tgcttctctt tgcgcgttct ttgcgtcctt
      301 tgcgtctttg cgttttcgtc tccttcatcg cccgtgcttc tgttcgaggg cggtcctgcc
      361 gaggaggccg aggaggacgt tgttaggggc ggggtgctcg gggtggaact ccatgcggac
      421 gcgctcgaag tattgaacgg tgtaggtctt gccgtcggag aggcgttcct ggatttcctc
      481 ggtgatgggg tagccgttta cgaacaggcc gccgtgctcc tgccagtact tgtagaaggc
      541 ggggctgacg ttatggccgg tctccgggaa atacttgtgg ccgggttggt tgttccgcgc
      601 cacaggggga tcgaccgcgt ggaactgcct gccgaggagg ctgagttgca cctcgtaggg
      661 ggtgcctttc tgttcggggt gtagctcgaa gcgggcgcgc tcgaagtact gcacggtgta
      721 gtctttgcca tcaatggggc tacgctcgac tgagggtgct gagatgggat agccaaactg
      781 ggcgaggccg ccgtattgcc gccagtagga ttccatcgcc ttgcataggg tatagcccgt
      841 ctctcggaag ggcgttccgc cgtcgcaggg acttggaccg accttgaaga gggctgaata
      901 ctgcctggtg atatcgaggt agcggttgcc gtaatcggcg gcaggctcga tctggctgcc
      961 ctcgaaccac tcgttccaac tggtgatggt gatccattcg gggttggcgg cgatggccgc
     1021 ccgccagttc tcctcgtaca gcgcaccgtc cttgcgcggt atgaccttga tgggggtgtt
     1081 aggctgcacc gacttgctgt cgtcccagcc gggcgtgacg cccccggtcc agagacgctg
     1141 cgtgccgtgc tgcttgttgt aagcgtccac ctttgcccgc cactggtttt ggacgcgggc
     1201 cacgtcggtg ttggcgtccc acttacccgc gctgaagaag tggatggtgt cgaacgcatc
     1261 caggtaagcg ccatctacgg tgtctaccga ccagagctgc gaattggcgg ggtctacctt
     1321 ctgccggacc gagcgccagg tggccgcgtc acccagggcg tcgggtcgcc agaagaagac
     1381 gacgggcttg ccgttccagc gcaggaaggc ggggtggctg ccaaagcgct ccatcacgga
     1441 gcggagctgg gacaccacgt cgcccttctg catgaccgag ttggtctcga agtaaattgt
     1501 tgcgcggaag ttgttgcggg cggcggcatc caggaggagg gggaagttgc ggtctgtctc
     1561 ggtgccgttg cccgtccatg agctaatgaa ggcgtcgatg cccgcccctt tggcctcttt
     1621 gacctgtcgc tcgacaacat ccgccttgtc cgagatatag ggcgcttgcg gcatgcccgc
     1681 cgtccggccc tggctgaagg agtcggggcc ataccacatg taatagaagg ccaatacgag
     1741 cttttcaccc gcaatggtgg acgtaggggc ggccagggaa gcaggtgcga cgccggaggt
     1801 gagcgtaagg agggcggcca gaatgagcaa ccatctaaac tggctgccca aacgcggttt
     1861 gatgatgaag ggcttgcgcg ccctgctggc ttgtttcaga tgttgcggca taggagaatt
     1921 gcaagcaggc tattgctctg cttgcaattc taccaatcta ccggccgaat gaacagggct
     1981 aaggggcggg actttagtac ctgatgtggt taccctttgt gctcaagata gaattggtac
     2041 ccctcgtaca ctcgcaggcc gccgtctttc acctcgacca cgcgctcagg gtagtcgtgg
     2101 aggaaggccc ggtcgtgggc gaccacgacg accgtgccct cgaacgcctc aagcgcctgc
     2161 tggaagtgct cgcggccgtc gatgtcgagg tggttcagcg gctcgtccag caggagcagg
     2221 ttgcagccgc gcagcacgag cacggccaat tgcagcctgc tccgctctcc cagcgagcac
     2281 tcgtcaaccc ggcggaagac gctgtcgccc tcgaatagga agaagtggag aaagctgcgg
     2341 gcctcggtct cgctcatggg acgctcgttg agcacggtct ggagcaccgt ccagtcgggg
     2401 cgtagcgtct cttgctcctg cgacagcctg cccaaccgca cgcctgagcc tatcttgagg
     2461 gtgccgccat cggggcgcaa cgtgccgtcg atcagcttga gcacggtaga cttgcccgcg
     2521 ccgttcggcc cgacgaatgc cacgcgctct ccgtagccta catcgaggga cgcgccttcg
     2581 aggatggtgg ggccgccggg gtagcggaac gtcacgtcct ccatgtggag caccgcgcgg
     2641 ccactggggg gtggggtgcc gaagtcgagc ttgagccacc aactgagggc aggcttgtcc
     2701 actcgctcgt ccgcctccat gtagcgttcg agcttggttt cgcggctctt ggcaagctgc
     2761 gccttgcggc gggccgacct gcggacgttg ggctggcggg gtgtggtgcc gcgctctata
     2821 tcgagtgccc agcccttcca gcgggcgatg tcggtcctga cgcgggagac gtattcctgc
     2881 tgtcgggtcc acgcctcgat atgggccagg cgttcttgct cgcgcgcctc ggcgaagtcg
     2941 ctgtagttgc cggggtagct tttggccgtg tgagtgccgg ggtcgaggta gatgacccgc
     3001 gttacggtgc ggtcgaggaa ctcgcggtcg tgggagacga taagcaccga gttggggtag
     3061 ctccccacga actcctcaag ccactccaac gcctccacgt ctaggtggtt ggtaggctca
     3121 tcgagcagca gcaggtccgg ctcctgcatg aggagggtag ccaggccgag ccgcgtcttt
     3181 tggcccccac tcagctcgcc cactcttgta taggccggga tgtcgcccag gcccagccct
     3241 tgcaggatgg cggcgctacg gtgctctcgc tcgtagccgc ccagcgcttc gtagcgggtg
     3301 agggcgtcgt tgtatgcctg catggcggct tcgacatcgg cggccgtggt gagggcgtcg
     3361 gccgcctgtt gcaaatcggt ctcggcggtc gcaagctcgc cctgtgcggc ggctaacacc
     3421 tcggagatgg tgcggcccgt gacgtccgcg aaggactgcg ctaggtagcc cacccgcagg
     3481 ccgcgcggtg acaggacgac cgtgccgccg tcgggtggtt cctggccggt aatgattttg
     3541 aggagggtag atttgccgga gccgttgggg ccgatgaggc caacgtgctc cccgtcgttg
     3601 acgatgaggt ttaccgcggc gaggactgtg ttgacgccgt agctcttaga aaggtgctgt
     3661 acttgcagca tggtgcctcc gccggagaca cttatgtctc cgcactagcg agtggtcagg
     3721 tcgagtgcta ttctggtgga ggcgctgcgc acaataggct ccttgctaag gccggtagac
     3781 gaccagccgc atctgcgggg cgatggggtc tggtagtgtt agtataaccg aaggtggagg
     3841 ggagggaata ggcttttaga tgtagataaa gaactggcaa gcgtaggata ttgcagcaat
     3901 ccaccgctcc ctttataaag tgggggaatc agggcaattc ttcggcttgt cattctgaac
     3961 gcagtgaaga atctcgtcct ccaccttact gttacccctt tcctacagga aagtgtaatt
     4021 ctaaagaaag gggctatttg ggtgctggag gacgagattc ttcactgcgt tcagaatgac
     4081 aaggtggagt tttgggtgct gtaggggcag ctgtcaaagg cgggcggctg atccttcgct
     4141 acgcgcatga tgacaagggg agagcgtggt tctgggtgcc taatttgcct cagccgtagc
     4201 agagcattga tttgctgccg tctgtcccag ccttcggggg aagctctaac gctcgtaagc
     4261 cgaggccgcc cggttcagcc ggtcgaatag ctgcctgcct gcttcctcta gctccggccg
     4321 ctcgttcatg agccgttcga ggtccaaggc cgcggcttct aggtgggtgc gggcgtcctc
     4381 gaatccggcg ttcagtgagg cacccttcag ggcggccccg tccgtgccgc gagtgtaggt
     4441 gacggcggac tttacggccc ggtcgtattc gctgcccgcg cctttggcgt cgccggaggc
     4501 caggagggca agggccaggt ccgctcgtgc gggcaagtaa tcgccgttta cctctacggc
     4561 ggcctggagg aacttgcgcg cgcggtcgta atcgcccgcc caggttgagg cggaacccag
     4621 gctatcgagg gcgagaatca tctgtgcgcc ttctgccttg cgtgcctccg cag
//
LOCUS       DASRWZ010000529         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_180456, whole genome shotgun sequence.
ACCESSION   DASRWZ010000529 DASRWZ010000000
VERSION     DASRWZ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180456"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VFH60_08255"
     CDS             complement(<1..1213)
                     /locus_tag="VFH60_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313812.1"
                     /translation="MRSSRIWGGLRAVVAWLRGEVATWLRLLRWWPAWATLVAVLLAL
                     TLAYQVPHTYSVDIGSAQDQLYVRNFHGRSEEETPSYRWSGVYSYVAFPGLGGSRPFT
                     ASLTLDPERTAPVTIIVNGETLFEGELQPGWQTLTLPVNEQHPGALASRDTVLEIRAP
                     DYRTPDAPGQPKGVKLDSAVVEQAAVGGFITPAYSALALLSGGVLLFYLFVGRAFASF
                     ASLKKARLWALFAALSLGVLLAVLLAQSHVAVAAAASHLLVTFASMILLFLAGSLLAG
                     RLVGRRYPVAAQGLALCVALAFGVRYGGMALPQSVIIDMPWHMKWLRTLLAGDWQSLY
                     FPGGLSAVPREWGLELLIPKSPLFYFTFAPLNLLPFGLETSTKWLICLLDATLVLAVF
                     WLILRVGGGRMS"
     gene            complement(1200..3248)
                     /locus_tag="VFH60_08260"
     CDS             complement(1200..3248)
                     /locus_tag="VFH60_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313813.1"
                     /translation="MILSRLLRIWLNPGIYAGVLLAGILMVGAYQFRPAYDIPFGTQI
                     DGVLLEGFHADEKAPDDQTFRFRWSSGDSYITLQDVGRQDFDVALSVSGTRPAGQPAP
                     SIEVSAGGKSLLKVEPPPQLTAYSFQVPREAVEDGTLRLHLTANAFSPPGDVRELGIL
                     VTHLRVTPSANPDRFIEPPLGPLVWVVGVVALLGLVLAALGWGVGGVALGTGVVGVFA
                     AWLLVADRLWLTSRQWYEAWPQTLLAGAALLLLFRPVSGWLLRIGGADWTAIQRRVFL
                     TVLFAAFVVRLAGQLHPQIYVVDLGFHEHRFQTVESGQLLFLTFSSEWGNRHNFYLPT
                     AYVFMLPLQWLLHDERLIIRIFTCAIATLGSFAVFYLVKRVLRSGWAALSASVLYLSM
                     PISVIPLSWGVTTNVFGEFFAVCALAIAVGAYPNLRPNRPALWALLFVFFVALLSHPG
                     VVQLTGLTFGLMSLIWLASRRMSGRKAQAAWLMGTLAAATMLAFTFYYSHFVQHMLTT
                     LEDIRREQASEAQKGGLHLLIGGSVSDRSLGLVVRYVETRSEWFFGGLRGFWQEAQAY
                     YRVWPMAGALLGYLLVWPASRKGVTLLRENARSRLALAALAWMLAVAVFALIGWTMNL
                     YVRYMLFALPAITIGAGILASGMRRAGRWGVVLSLMLVAYFVVEMLALWHYRINYALK
                     "
     gene            complement(3245..>3658)
                     /locus_tag="VFH60_08265"
     CDS             complement(3245..>3658)
                     /locus_tag="VFH60_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313814.1"
                     /translation="VLDDFILVKEFGPDRVYRLDGPSDLADMKALIPEGAPVLLSAPE
                     YDPERGQGALVYGGYMAAVGYFLRDHPLYGDARLSFGQPIREFNPANRTEYAVLWAAQ
                     DPTLSGYKPGTWIWSNEFVALYKHGPGIASTGRAP"
BASE COUNT          837 a         1223 c         1055 g          543 t
ORIGIN      
        1 ccgacatgcg cccgcctccc acgcgcaaaa tcagccaaaa aacagccaga acaagcgtgg
       61 catctaataa gcagattagc catttggttg atgtttcaag accaaaaggt aacaaattaa
      121 gaggtgcaaa ggtaaaatag aacagcggcg atttggggat cagcaactcc aatccccact
      181 cgcggggcac agcgctcagt cccccaggga aatagagcga ctgccaatcc cccgccagca
      241 gcgtcctcag ccacttcatg tgccagggca tatctatgat aaccgactgt ggcagggcca
      301 tgccgccata gcgcacgccg aacgccaggg caacacacag cgccaagcct tgtgctgcta
      361 caggatacct ccgccccacc aacctacccg cgagtaagct cccggccaga aagagcaaaa
      421 tcatgctcgc aaatgtaacc aacaggtgac tcgccgcagc tgctaccgcg acgtggctct
      481 gcgccaacaa cactgctaat agtacaccca gggataaagc cgcaaacaac gcccacaagc
      541 gcgctttctt gagcgaagca aagctggcaa aagcacgccc cacgaatagg tagaagagca
      601 atactccgcc ggacaacaac gccagggccg aatacgcagg agtgatgaag ccgcctacag
      661 ccgcctgctc cacaaccgcg ctgtccagct tcaccccttt gggctggcct ggcgcatcgg
      721 gcgtgcggta gtcgggggcg cgtatctcaa gcaccgtgtc gcgggaggcc agcgcgcccg
      781 gatgctgctc attgacaggc agagttagcg tctgccaacc cggctgcaat tcgccctcga
      841 acaaagtctc gccgttgacg atgatggtga ctggggccgt gcgctcaggg tcgagggtca
      901 ggctggcggt gaatgggcgg ctgccaccca ggccggggaa ggccacgtag ctgtagacac
      961 cggaccacct gtaagaaggg gtctcctctt cgctgcgccc gtgaaagttg cgcacgtaaa
     1021 gttggtcctg cgcgctacct atatccaccg agtaggtatg tggcacctgg taagccaacg
     1081 tcagggcaag cagtaccgcc accagcgtgg cccaagcagg ccaccatcga agcagccgga
     1141 gccacgtagc gacctccccg cgcagccagg ccactacggc gcgtagaccg ccccaaatcc
     1201 tacttgagcg cataattgat cctgtagtgc cacagcgcca gcatctcgac gacgaagtac
     1261 gccaccagca tcagggagag tacgactccc caccgcccgg cccggcgcat acccgatgcg
     1321 aggatgcccg cacctatggt tatcgcgggc agcgcgaaga gcatgtatcg cacgtagagg
     1381 ttcatagtcc agcctatcaa ggcgaacacc gcgacggcca gcatccatgc cagcgccgcc
     1441 agcgccagcc ggctgcgggc attttccctg agtagcgtca cacccttcct gctcgcaggc
     1501 cacaccagga ggtagccgag caaagcgccc gccatgggcc acacgcgata gtaagcctgc
     1561 gcctcctgcc agaagccgcg cagcccaccg aagaaccact cactcctggt ctccacgtat
     1621 cgcaccacca ggcccaggct gcgatccgac accgagccgc cgatgagcaa atgcagcccg
     1681 cccttctgcg cctcggatgc ctgttcccgg cgtatatcct caagggtagt gagcatatgt
     1741 tggacgaagt ggctatagta aaaggtgaaa gccagcatgg tagccgccgc cagggtgccc
     1801 atgagccatg cagcctgggc cttccgcccc gacatacgcc tcgacgcaag ccatatgagc
     1861 gacatcagcc caaaagtcag ccctgtaagc tgcaccacgc ctgggtgact cagcagggca
     1921 acaaagaaca cgaatagcag cgcccagaga gcgggacggt tgggccggag gtttgggtac
     1981 gcgcccacgg cgattgccag cgcgcatacc gcaaagaact ctccaaacac gttggttgtc
     2041 acgccccacg acaagggtat cacggaaatg ggcatgctca ggtagagcac gctggccgac
     2101 aaagcagccc agccgctacg cagcacgcgc ttcaccaggt agaacaccgc aaacgagccg
     2161 agcgtggcaa tagcgcacgt aaagatgcgt ataatcaacc gctcgtcgtg cagcagccac
     2221 tgtaggggca gcatgaagac gtaagcggtg ggcaggtaga agttgtggcg gttgccccac
     2281 tcggaggaga aggtcaggaa gaggagttgg cccgactcca ctgtctggaa tctatgctcg
     2341 tggaagccca ggtcaaccac atatatctgc gggtgcaact gccccgccag cctcaccacg
     2401 aaggccgcga agagcacggt caggaagacg cggcgttgaa ttgccgtcca gtccgccccg
     2461 ccaatgcgca gcagccagcc acttaccggc ctgaacagca gcaacaacgc cgccccggcc
     2521 aatagagtct gaggccaggc ttcataccac tgccgggaag tgagccacag ccggtcggca
     2581 accagcagcc acgcggcaaa aacgcccacg acccctgtgc ccagcgcaac gccgccgact
     2641 ccccagccga gcgccgccag caccaacccc agcagtgcaa ccacgcctac cacccacacc
     2701 agcggcccca ggggaggctc gatgaaacgg tcggggttgg ccgaaggggt cacacgcaga
     2761 tgcgttacca ggatgccaag ctcccgcacg tcgccgggcg gggagaaggc gttggccgtg
     2821 aggtgcaatc tcagcgtgcc gtcctcgact gcctcacgcg gcacctggaa cgagtaggca
     2881 gtcaactgcg gaggcggttc tactttcagc aggctcttac ctcccgcact cacctcaatc
     2941 gaaggtgcag gctgtcccgc cgggcgcgtg ccgctgaccg atagcgccac gtcgaagtcc
     3001 tgccgcccca cgtcttgcag agtgatatat gagtcgccgg acgaccacct gaaccggaaa
     3061 gtctggtcat ccggcgcttt ctcgtccgcg tggaagccct caagcagcac gccgtcaatc
     3121 tgagtgccga aggggatgtc ataggcagga cggaactggt acgcgcccac catgaggata
     3181 cccgcgagca gcactcccgc gtagatgccg gggttcagcc atatccgcaa caagcgcgac
     3241 agtatcaagg cgcgcgcccc gtcgaggcaa tgcccggccc gtgcttatag agcgccacga
     3301 attcgttaga ccaaatccag gtccccggct tataccccga cagggtgggg tcctgcgccg
     3361 cccacagcac ggcatattcg gtacggttcg ccgggttgaa ctcacgtatg ggttggccga
     3421 agctgagccg cgcatcgccg tagagcgggt ggtcgcgcag gaagtaaccc accgccgcca
     3481 tgtatccccc gtatacaagc gcgccctgcc cccgctcagg gtcgtactcc ggtgcgctca
     3541 ggagcaccgg cgcgccctcc ggtatcagtg ccttcatgtc tgcgaggtcg ctcggcccat
     3601 cgagcctgta aacgcggtcg gggccgaact ccttcaccag tataaagtcg tccagcac
//
LOCUS       DASRWZ010000530         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_591811, whole genome shotgun sequence.
ACCESSION   DASRWZ010000530 DASRWZ010000000
VERSION     DASRWZ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591811"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFH60_08270"
     CDS             complement(<1..366)
                     /locus_tag="VFH60_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HET6313815.1"
                     /translation="MSEQAALTGETAVVIVPPHDICGFADHYRRLYMPGTLHRIPPHI
                     TLAYPFVPFDELEAAMPRLADALAACPPRAVAIRGFSIFQDAGILYLRVANDERVLSL
                     HQAIMAEFPDYPIYGGQLTT"
     gene            complement(363..1577)
                     /locus_tag="VFH60_08275"
     CDS             complement(363..1577)
                     /locus_tag="VFH60_08275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET6313816.1"
                     /translation="MPGIRSRAIGIGTRVAGLPWRLRPGRGRPLSPADPRLKRVVVIK
                     PCCMGDLLMSTPAIAALRNALPNSHISLAVGEWSRPAVGNNPHLNSLLDSPVGGANTL
                     PASYLRLARHIRRGRYGAALVLDRSPLLNLVPYLAGVSVRAGLDSDGRGLALTHPVNC
                     PALRSRHEVEWYLDVVRALGLPAPPEDAHLEFYPTQDDREQAARALSQAVGEEGESEL
                     GCVAIHLGGGSNPGMRLLSKRWEPGRWARIADWLAETYEPSILLLGGPGKEDREAAEA
                     VKSALFPATRPYVADLVGKLEWGSMAALIERCSLFLGHDTGAMHLATAMRTPTVAVFG
                     PSDPARYGPWDPSGRSMAVAPRRAASGAEALRQVSRSSNQLYHAEVTVEEVWSAVEKV
                     YAKAMSGERVRA"
     gene            complement(1550..2485)
                     /gene="prmA"
                     /locus_tag="VFH60_08280"
     CDS             complement(1550..2485)
                     /gene="prmA"
                     /locus_tag="VFH60_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET6313817.1"
                     /translation="MNWLELAVEVDPEAVESISELFARYGYNGGVVVEQPLVSPQAEA
                     QDWEQLLQPEIDPTRPVSVRTYLACDEDAPQKQRQIEEAVWHLGQMRQVGPLRVRECR
                     EEDWANAWKDYYGIQRMGERIVIKPSWLQYEPREGDVILDLDPGMAFGTGLHPTTRLC
                     LAALEEYVRPDMKILDLGTGSGILAIAAAKLGGPSVRVTALDTDIIAVEATQQNVERN
                     GLARQIELEQGSTLVAQHDGPYTLIVANILASVIIELARSLYELVEPGGTLITSGIFI
                     DRGEKVVEALERAGLPVRERRQEGDWLCLVSVRER"
BASE COUNT          430 a          794 c          825 g          486 t
ORIGIN      
        1 tgtagtcagt tgcccaccat agatagggta atcggggaac tcggccatga ttgcttgatg
       61 gagggaaagc acgcgttcgt cgttggcgac gcggaggtag agtatacctg cgtcctggaa
      121 gatggagaag ccccttatcg cgactgccct gggtgggcat gctgcaagcg cgtccgctaa
      181 cctgggcatg gcggcttcta actcatcgaa gggtacgaac gggtaggcca gggtgatgtg
      241 gggtgggatg cgatgcaggg tgccgggcat gtagaggcgg cggtagtggt cggcgaaccc
      301 gcagatgtcg tgcggaggca ctatcacgac ggcggtctcg cccgtgaggg ccgcttgctc
      361 gctcatgctc gcaccctctc cccggacata gccttcgcgt acaccttctc tacggcggac
      421 catacttcct ctaccgtgac ttcggcatgg taaagctggt tgctgctccg cgatacctgc
      481 cgcagggcct ccgcgccgga agccgcacgc ctgggcgcga cggccatgct gcgaccggag
      541 gggtcccagg ggccgtagcg tgcagggtcg ctggggccga agaccgccac agtgggcgtt
      601 cgcatagccg tcgccaggtg catagcgccc gtgtcgtgcc ccaggaagag gctgcacctc
      661 tctatcaagg cggccatgct gccccattcg agcttgccta cgaggtcggc tacgtagggc
      721 cgggtggcgg ggaagagcgc cgacttgact gcttcggctg cctcgcggtc ttccttgccg
      781 ggaccgccca gcaggagtat agaaggctcg taggtctcgg ccagccagtc ggctatgcgc
      841 gcccaccgcc ctggctccca ccgcttggag aggaggcgca tgccggggtt ggaaccgccg
      901 cccaggtgga tagccacgca gcctaattcg ctctcgcctt cttcccctac ggcctgtgat
      961 aacgcccgcg cagcctgctc cctgtcgtcc tgggtggggt aaaactcaag gtgggcatcc
     1021 tccggtggag cagggaggcc gagggcgcgc actacgtcca ggtaccactc tacttcgtgg
     1081 cgggagcgca aggccgggca gttgacgggg tgggtgaggg caaggccgcg cccgtcgctg
     1141 tccagcccgg cgcgcacgga cacacccgca aggtagggca cgaggttgag caggggcgaa
     1201 cggtccagca ccagcgcggc accatagcgg ccacgtctaa tatgccgtgc caggcgcagg
     1261 tagctggccg gcagggtatt cgcgccccct acaggcgaat ctaaaaggct gttcagatgg
     1321 gggttgttgc cgacggctgg gcgcgaccac tctcctacag ctagagagat gtgcgagttg
     1381 gggagtgcat tgcgtagggc ggcgattgca ggagttgaca taagcaggtc gcccatgcag
     1441 caaggcttga tgacgaccac gcgcttcaaa cgagggtcgg cgggtgagag gggtctgcca
     1501 cgtcccggtc taagccgcca gggtagcccc gccacccttg tgcctatgcc tatcgctctc
     1561 gaacggatac caggcaaagc caatctccct cctgccgcct ctcgcgcacc ggcaggcccg
     1621 ccctctccaa cgcttctact accttctcgc ccctatctat gaaaataccc gatgtgatga
     1681 gcgtgccgcc cggctccacc aattcataga gcgacctggc tagctcgatg atgacgctgg
     1741 cgaggatgtt cgccacgata agggtgtacg gcccatcgtg ctgggctacc agggtggagc
     1801 cttgttctag ctctatctgc cttgcaaggc cgttgcgctc tacgttctgc tgggtggctt
     1861 caacagcaat aatgtcggtg tcgagcgccg ttacgcgcac gctagggccg cccagcttcg
     1921 cggctgctat cgccaggatg ccggagcctg tgcccaggtc gagtattttc atgtcaggcc
     1981 gcacgtactc ttcgagcgcg gccaggcaga ggcgcgtcgt tgggtggagg cccgtgccga
     2041 aggccatgcc ggggtcgagg tcgagtatta cgtcgccctc tctcggctcg tactgtagcc
     2101 aggagggctt gatgacgatg cgctcgccca tgcgctggat gccgtagtag tccttccagg
     2161 cgttggccca gtcctcttcg cggcactcgc gcacgcgaag aggcccgacc tggcgcatct
     2221 gccccaggtg ccacacggct tcctcgattt gccgctgctt ctgaggcgcg tcctcgtcgc
     2281 atgccaggta ggtccgcacg ctgacgggcc gcgtggggtc tatctcgggc tggagaagct
     2341 gctcccagtc ctgcgcctcg gcctgcgggc tgaccagcgg ctgctccacc actacgccgc
     2401 cgttgtaccc atagcgcgcg aaaagctcgc tgatggattc aaccgcctcc ggatcgactt
     2461 caactgccaa ctctaaccag ttcatcttaa ctttgaattt tgagtcttga atatcaagat
     2521 tacttaaacg tttcg
//
LOCUS       DASRWZ010000531         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_111917, whole genome shotgun sequence.
ACCESSION   DASRWZ010000531 DASRWZ010000000
VERSION     DASRWZ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111917"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VFH60_08285"
     CDS             <1..1127
                     /locus_tag="VFH60_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6313818.1"
                     /translation="RPRAYTRDLEWGVPIPIEGYEDSGKRIYVWFDAVKGYLSATMEW
                     AMLKGEPDAWKEWWLQEEGEPLPSKSYYFIGKDNIAFHTIIWPSMLLGHGGLALPYDV
                     PANEFLTGETGEKASSSRGGGGVPWLPDYLDRYKPDPIRYFMTSNAPETRDTVFSWAE
                     FYRRNNEELVATYGNLVHRVLTFTSRNFDGKVPQPGELTEQDRTLTANATAAFERVGD
                     LIAACRFKEALREVMGLATEANRYLDDTAPWKAIKVDRERAGTALYVTLTVINALKIL
                     TAPFLPFSAQQLHEMLGFEGDVHQERWASPELPPGQALGTPAPLFDKLDEANLEAENA
                     RLGDPWVDPEGPVAQQGPQERVVIFEGQIRTRKELDEDWA"
     gene            1443..>2239
                     /locus_tag="VFH60_08290"
     CDS             1443..>2239
                     /locus_tag="VFH60_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6313819.1"
                     /translation="MKVLVTGGAGFLGINLIRYLHARGHECVSLDLIDFDYPDMVDKV
                     TAVKGDIRKDKDVQKAMQGVDQVVHCAAALPLYSPKDIYTTDVEGTRMVLEVALRNKV
                     QRAVMISSTAVYGIPDHHPLVENDQLEGVGPYGQAKIQAEMVCLEYRAKGLVVPIIRP
                     KSFIGPERLGVFAMLYDWAHTGHNFPMIGSGKNRYQLLDVEDLGQAIYLCMTLPEEKV
                     NDTFNIGAKVFTTMKKDYQAVLDYAGHGKKILGFPAAPMIWTLRLLEA"
BASE COUNT          479 a          710 c          675 g          375 t
ORIGIN      
        1 tgaggccgcg cgcctacacc cgcgacctcg agtggggcgt gcctatccct atagagggct
       61 atgaggacag cgggaagcgc atatacgtct ggttcgacgc agtgaagggc tacctctcgg
      121 ccactatgga gtgggcgatg ctcaaggggg agcctgacgc ctggaaagag tggtggctgc
      181 aagaggaagg cgagccgctg ccgagcaaat cgtactattt catcggcaag gacaatattg
      241 cctttcacac catcatctgg ccgagcatgc tgctcgggca cggcggcctc gcgctgccct
      301 acgatgtgcc cgctaacgag ttcctcaccg gggagacggg cgagaaggca agctccagcc
      361 gcggcggggg cggagtgccc tggctgcccg actacctcga ccgctacaag cccgacccca
      421 tccgctactt catgacgagc aacgccccgg agacccgcga cacggtcttt tcatgggccg
      481 agttctaccg ccgcaacaac gaggagcttg tcgccacgta tggcaacctc gtccatcgcg
      541 tgctcacctt cacttcgcgc aacttcgatg gcaaagtgcc ccagccgggc gagcttaccg
      601 agcaggaccg caccctgacc gctaacgcca ccgccgcctt cgagcgcgtg ggcgacctca
      661 tagccgcgtg ccgcttcaag gaggcgttgc gcgaggtgat gggcctggcg accgaagcca
      721 accgctacct cgacgatacc gccccctgga aagccatcaa ggtggaccgc gagcgggcgg
      781 gcaccgcgct gtacgtaact ttgaccgtca tcaacgcgtt gaagatactg accgctccct
      841 tcctgccctt ctcggcgcag cagctacacg agatgctggg cttcgagggc gacgtgcacc
      901 aggagcgttg ggccagcccg gagctacccc caggccaggc gctcggcacg cctgccccgc
      961 tgttcgacaa gctggacgag gccaacctgg aagccgagaa cgcgcgcctg ggcgacccct
     1021 gggtggaccc ggaaggtccg gtcgcgcagc aaggcccgca ggagcgcgtg gtcatattcg
     1081 aggggcagat acgcacccgc aaagagcttg acgaggattg ggcgtagtag aggaccagga
     1141 ttgacaccag ggtgtaccgg caaagcgcgg tcttgcgctc ccaaaggtgg accttgtcat
     1201 tctgagcgta gcgaagaatc aggtgggtag ctaagctgtt gaatgtagga gcagattgga
     1261 agtaactagg aagatagtgt aagttgtaag taccaaacat gggtggctga ttcttcgcta
     1321 cgctcagaat gacaaggtcc acctttggtt attctctttc aacagtttgc agctactccc
     1381 tgatacaaga gcgcaatagt cacctatcct caaggacata gaaggagacc aaccaaccac
     1441 acatgaaagt actagtcacc ggaggggccg ggttcctcgg catcaacctt attcgctacc
     1501 tgcacgcacg cggacatgag tgcgtatccc tcgacctcat cgacttcgac taccccgaca
     1561 tggtggacaa ggtcaccgcc gtcaagggcg acattcgcaa ggacaaggac gtacagaagg
     1621 cgatgcaggg cgtggaccag gtggtgcact gcgccgccgc tctccccctc tactcgccca
     1681 aggacatcta caccaccgac gttgaaggca cccgcatggt gcttgaggtg gccctgcgca
     1741 acaaggtgca gcgcgcggtc atgatctcat ccaccgccgt ctacggcatc cccgaccacc
     1801 accccctcgt ggagaacgac cagctggagg gcgtagggcc gtacggtcaa gccaagatac
     1861 aggccgagat ggtgtgcctg gagtaccgcg ccaagggcct cgtcgtgccc atcattcgcc
     1921 ccaagtcgtt catcggcccg gagcggctgg gcgtgttcgc catgctgtac gactgggcgc
     1981 acaccgggca caacttcccc atgatcggca gcggcaagaa ccgctaccag ctactcgacg
     2041 tggaggacct cggccaggcc atctacctct gcatgacgtt gccggaggag aaggtcaacg
     2101 acaccttcaa cataggagcc aaggtcttca ccacgatgaa gaaggactac caggccgtgc
     2161 tcgactacgc cgggcatggc aagaagatac tgggcttccc cgccgcgccc atgatctgga
     2221 ccttgcgcct gctcgaagc
//
LOCUS       DASRWZ010000532         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_180629, whole genome shotgun sequence.
ACCESSION   DASRWZ010000532 DASRWZ010000000
VERSION     DASRWZ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180629"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1600)
                     /locus_tag="VFH60_08295"
     CDS             complement(329..1600)
                     /locus_tag="VFH60_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HET6313820.1"
                     /translation="MPGTYSNKDSFLEDLAARVQLRGGPQDAERVLRAIFEHEQEPGA
                     DALTERVLARIARMPVPVIAAMRRELEQAEMLEPGLSIQMTPAARQTLAEEWGWQPLQ
                     QTAFLSPAPRPSRSEEARPQEPQAGQVCRACEGTGVAPFGPQWDEVLAGLQKHFLGKP
                     RKVQAGGRARSQPTPDTNLRRAALLHEQGALAGKDVLVLGEDVTVAAAVALAGKALSP
                     SGRLARRLVALHSDDKTLRQLRDIAVGEGLLIGLVTHDLRRPLMPDLQGEFDTVFVDA
                     PQSYAGAVLALSRAIDAANVEGTIYLSYGSERQADLLEVQRAMLEAGLLIAQVTPGFD
                     KHSTGSRDLYVLRMTEDSLPLVEGDYTEEISDERQTYACTNCGRQLTVGADVGGRFAT
                     LPDLEEAGCPTCGGRTFTLIPAHHPGGNGSG"
     gene            complement(1604..>2519)
                     /locus_tag="VFH60_08300"
     CDS             complement(1604..>2519)
                     /locus_tag="VFH60_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET6313821.1"
                     /translation="LMQVGEFWVRFGIDGWRLDVPSEINDDTFWREFRTRVKAINPDA
                     YIVGEIWHEARRWLQGDQFDAVMNYLFTKLCIEFFIGSNVDPALVEGSSLWPVRHIEA
                     AEFGKEIDALLKLYSREITEVQMNLLDSHDTARYLTMARDDKSALQLATLFQMTYPGA
                     PSVYYGDEVSLAGGKDPLSRASFPWDQAAWDTDMRAFFKRVVALRHAHPALRRGDYQT
                     LFAQGHLYVMGRQLGGDLVVVAFNTGVEPVSAAIPVAPFLQNGLVLRDEWTGQSLQVD
                     GGLLSLSLAARSGAVLVLADAPAGGYAG"
BASE COUNT          425 a          865 c          807 g          422 t
ORIGIN      
        1 gtgctggacg aaatgcgccg cgccgccagc atggccgaag tagaccgcag ccacgaccac
       61 cccgcacgct gccgcgcctg cggctaccgc cccatctgcg accagtccct ctggcccacc
      121 aactccgatt agacgagaaa tgcaaccgca aaggcgcaaa gagcgcgaag gacacggaaa
      181 cataaagtag gtgttctacg caaaaggacc agggtacgac aacacgtacc ctggtccttt
      241 ttgtacccgt taaacaatcc ttaaactttg cgtcctttgc gctctttgcg cctttgcggt
      301 ttcgtctttt cctgtgtccc agcctgcgtc agccggagcc gttgccgccg gggtggtggg
      361 cggggatgag ggtgaaggtg cggccgccgc aggtggggca gccagcctcc tccagatcgg
      421 ggagggtggc aaagcggccg ccgacatccg cgccaacggt tagctggcgg ccgcagttgg
      481 tgcaggcgta ggtctgccgc tcgtcggata tctcttctgt gtagtcgcct tcgaccagcg
      541 gtaggctgtc ctcggtcatg cggagcacgt agaggtcgcg gctgccggtg gaatgcttat
      601 cgaagccggg ggttacctgc gcgatgagca ggcccgcttc cagcatggcg cgttgcacct
      661 ctagcaggtc ggcctggcgc tccgagccgt aggagaggta gatagtaccc tccacgttcg
      721 ccgcgtcgat agccctggac agcgcgagca ccgccccggc gtagctctgc ggcgcgtcca
      781 cgaacaccgt gtcgaactca ccctgcaagt cgggcatgag ggggcggcgg aggtcgtgcg
      841 tcaccaggcc gatgagaagc ccctccccga ccgcgatgtc ccttagctgc cggagcgtct
      901 tatcgtcgga gtgcagggcc accaggcggc gggccagcct gcccgaaggt gacagcgcct
      961 tgcccgccag tgccaccgcc gccgctaccg tcacgtcttc gcccagcacc agcacgtcct
     1021 tgcccgccag cgcaccctgc tcgtgcagca gcgcggcccg gcgcaggttg gtatcgggcg
     1081 tgggttggct cctcgccctg ccccccgcct gcaccttgcg cggcttgccc aggaaatgct
     1141 tttgcagccc tgccagcacc tcatcccatt gcggtccaaa aggcgctaca cccgtgccct
     1201 cacacgcccg gcacacctgc ccggcctgcg gctcctgggg acgggcttcc tccgaacgtg
     1261 aagggcgagg tgcgggcgac aggaacgctg tctgctgcaa aggctgccag ccccattcct
     1321 cggccagcgt ctgcctggcg gcgggggtca tctggatgga cagccccggt tccagcatct
     1381 cggcctgctc tagctcccgc cgcatagccg cgattacggg caccggcatg cgcgcgatgc
     1441 gggcaagcac ccgctcggtc agcgcgtccg caccaggctc ctgctcgtgc tcgaagatgg
     1501 cccgcaacac ccgctcggcg tcctgggggc cgccccgtag ctgcacccgc gcggccaggt
     1561 cctcaagaaa gctgtctttg ttcgagtaag taccgggcat agctcaacct gcatagccgc
     1621 cggccggtgc gtccgcgagg acaaggaccg cacccgaccg ggccgcgaga ctcagtgaca
     1681 gcaagccgcc gtctacctgc aacgactgtc ccgtccactc gtcgcgcagt accaggccgt
     1741 tttgcaggaa gggggctacc gggatggcag ccgacaccgg ctcgaccccc gtgttgaacg
     1801 ccaccaccac gagatcgcca ccgagttgcc tgcccatgac gtagaggtgg ccctgcgcga
     1861 acagagtctg gtaatcgccc cggcgaaggg ccgggtgggc gtggcgcagg gcgactaccc
     1921 gcttgaagaa ggcccgcata tcggtatccc aggccgcctg gtcccagggg aaggaggcgc
     1981 gggaaagagg gtccttgccc cccgctaggc tcacctcgtc accgtagtat accgagggcg
     2041 cgccgggata ggtcatctgg aagagggtgg cgagctgcag agcgcttttg tcgtcgcgcg
     2101 ccatagtcag gtagcgggcg gtatcgtggc tgtcgagcag gttcatctgc acctcggtga
     2161 tctcgcggct gtagagcttg agcagggcgt ctatctcctt gccgaactcc gccgcctcga
     2221 tgtgccgcac cggccacagc gacgatccct ccacgagcgc ggggtccacg ttgctcccga
     2281 tgaagaactc tatgcacaac tttgtgaaca ggtagttcat caccgcgtca aactggtcgc
     2341 cctggagcca gcgccgggcc tcatgccaga tctccccgac gatgtaggcg tccgggttga
     2401 tcgccttgac ccgcgtgcgg aactcgcgcc agaaggtgtc gtcgttgatc tcggagggca
     2461 cgtcgagccg ccagccgtcg atgccgaagc gcacccagaa ctcgcccacc tgcataagg
//
LOCUS       DASRWZ010000533         5838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1208660, whole genome shotgun sequence.
ACCESSION   DASRWZ010000533 DASRWZ010000000
VERSION     DASRWZ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208660"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFH60_08305"
     CDS             complement(<1..224)
                     /locus_tag="VFH60_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313822.1"
                     /translation="MFPVLYQNGPIIVFTHDFFTLLGLVVGLLVYYRELRRRGMLSYQ
                     IFWISMAAIVGGGIGARLSVVWEHPAYYAN"
     gene            complement(647..3382)
                     /locus_tag="VFH60_08310"
     CDS             complement(647..3382)
                     /locus_tag="VFH60_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6313823.1"
                     /translation="MVGALQGGDNPRPGHASLYPASQQGRRHVWRDWTGRSIAKAIGA
                     FVMGETTFGQWLKERRKALDLTQEELAERVGCSVWMVQKIETGDRRPSRQVVVLLADA
                     LRIPPDRREAFMSLAREGRAAGEQITALERREEPPHPGGTVQPPQPAEPPAPPRTPTN
                     LTLPPTPLIGRNEDLAAIRARLLRESVRLLTIVGPPGIGKTRLAVEIAHDMLPDFAGG
                     VFFVPLAPISDVGLVSSTIAHTLDLKESPGLSVEAALVEHLRDKKMLLVLDNFEQVVG
                     AAGVVARLIAHCASLKVLVTSREPLHLRGEQQFPLQPLGLPGPGALPPIDALTAYPAI
                     GLFVERAQEVSSFELTHHNARPVAEICAHLDGLPLAIELLAARVKLLSPQALLARLTG
                     PAAAGSNGAGSGDAGSLMLAAKGARDLPDRHQTLRTAIAWSYDLLKEGERKLFTRLGV
                     FVGGFTLAAVDAVCNAEGDLGFDTLEAVESLLDKSLLKLGRAGRDVEAYAAANEEPRF
                     YMLETIREYARERLLESSEIDKVRRWHAEYYLAMTEAAESELRGSRQQEWLARLEDEH
                     ENLRAVLDWSLANGEAALGVRLAGAIWRFWWMRGYLTEGRRWLDMALTRVPERTADRA
                     RAAYGASILAKVQGDSATSIRLEAEGLAIYRELGDGRGVAQVLLGMGLRQRAGGELEA
                     AEASFKESLELSRESGDTWGIAGALTNLGLIFFGRDDEAARTYLEEGLQAMRRTGDRN
                     NMTLILNNLGVIALHRGNLGEARSLWDENLRVYRELANEENQALTLSNLALVAREEGQ
                     LTEARELSLQSLDIYFRLGNTDYLAPGLERAASIAALQRRPVRAARLYGAADAVYEAA
                     RLARQPYNLLDYERMMVLIRESLDEAELAKATEEGRAMSVAEAVAYAREDGSDSL"
     gene            3553..3810
                     /locus_tag="VFH60_08315"
     CDS             3553..3810
                     /locus_tag="VFH60_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313824.1"
                     /translation="MTNQAHRSDKQRDNTQHEHQGNGTGEPLGPSQLFLVRVWAEQGM
                     EGSPEWRGRVQHVTRGDAQEFSDWGTLIDLFVTMVPALESK"
     gene            3879..>5838
                     /locus_tag="VFH60_08320"
     CDS             3879..>5838
                     /locus_tag="VFH60_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313825.1"
                     /translation="MVANLGQYKRQVVRFISALLLVVFLASASWSPAGAASMASPNAS
                     PEAVTVHCVDDNGTTTGTPCANATPYNNIQAALDAASPGDEVRVAVGTYVRAAGRGAT
                     PPNNVITITGGYAGGSAAGGWTVKGPATGTVLVGTNFNLPPGVVVTLQNLVVSNASID
                     NQGGTLHVATGDLHLQGGTSKGAFDIEAGAKVDFSQGSHTLSNGTTFAGSGTVRVSGG
                     TLVVGSSATERVAARRVELASGTLSGPATLAISNTLTWTGGTMSEAGKTELVPGATLS
                     ISGAAAKTLNARTLNNQGTATWLGAGAISLEAGASIHNSGLFDAQNNATLSAPGPAAF
                     YNTGTFRKSASTGVTTIHSNVKFENNGGTVDVAGGTLRFGTASVAGASSEVSAPEIRF
                     DDPNFMNSNIRVGNTSAASSPQASNAVLEFASGTFDFQGSNTVVGDGVARVNGASLRV
                     QGTLAARNLELASGLIDGAGTLAVSGAFNWSGGQMSGAGATNISNGASLNLSGSNVKT
                     LSARTLNNQGTATWLGAGAISLAAGATLNNSGLFDAQNNAAITAPGPATFRNTGTLRK
                     SASTGVTAIDSAVKFENDKGTVEVAGGTLRFGAASTAVAPSKVSSPDGAAGTIWISSG
                     LKIGNTAAASGPQASNAVVEFAGGVHSFE"
BASE COUNT         1101 a         1930 c         1762 g         1045 t
ORIGIN      
        1 atgttcgcgt agtaagccgg gtgctcccag accacgctca ggcgagcgcc gatgccccct
       61 cctacgatcg cggccataga tatccagaag atctggtagc tcaacatgcc acgtcgccgc
      121 agttcgcggt aatagacgag cagccccacc accaggccga gcagggtgaa gaaatcgtgc
      181 gtgaacacga taataggtcc gttctggtaa aggaccggga acatgcgcta cctcgttttg
      241 cctgctgtca ggcggcggcg atgcgagcaa agggaagctt agattggagc gattatacag
      301 cagccgcccg ccggtgtcta tgcttggcga cgttgatacc agggcttaat cattcgctgt
      361 agccaccgct acggtagccc tgtggctaaa gccacgggct tacaagaggc aaagtcccct
      421 acggggactg aggaacatca tgtagtctcc gaaggagact tcgtctctgg taagcccgcc
      481 aattcattgg cagggcaatc ccacagggct accgtggccg gcacccatgc aggaccacct
      541 accgcctcgc caccgagcga aaatgatgaa gccctaactt tcatactgac tctcaagcaa
      601 cggagctata ctggctgcct cgaccaaggg gctgaccgtg ccgtcgctag aggctgtcgc
      661 ttccgtcctc gcgtgcatag gcgaccgctt cagccactga catagcgcgc ccctcctcgg
      721 tggctttcgc aagctcggcc tcatccaggg attcccgtat caaaaccatc atccgctcgt
      781 aatccagcag gttatagggc tgcctggcga gacgggcggc ctcatacaca gcgtcggccg
      841 ccccatacaa cctggcggcc cgcaccggac ggcgttgcag cgcggctata cttgcggcgc
      901 gttccaggcc gggtgccaga taatcggtgt tccccaaccg gaagtaaatg tccaggctct
      961 gcaagcttaa ctcgcgggcc tcggttagct gcccttcctc tcgcgccaca agcgccagat
     1021 tggacaaggt aagggcctgg ttctcttcgt ttgccagctc gcggtacacg cgcaggttct
     1081 cgtcccacag actgcgcgcc tctccaagat tgcctctatg tagagctatc acccccaggt
     1141 tgttaagtat caaggtcata ttgttgcggt cccctgtccg tcgcatcgcc tgcaggccct
     1201 cttccaggta ggtacgcgcg gcctcgtcat ctctgccgaa gaagatgagg ccgaggttgg
     1261 tgagcgcacc tgcgattccc cacgtatcac ccgactccct tgaaagttcc aggctctcct
     1321 tgaagctagc ttcggcggcc tccagttctc caccggcacg ctggcgcagg cccatgccga
     1381 ggagcacctg ggccacccca cgcccgtccc ccagctcccg gtatatagcg agaccctcgg
     1441 cttctaaccg gatgctcgtg gcgctgtctc cctgcacctt cgccaggata gacgcgccat
     1501 aggccgctcg cgcgcggtcc gcggtgcgct ccggcaccct cgtcagggcc atatcgagcc
     1561 accggcgtcc ctcggtgagg tagcccctca tccaccagaa ccgccagatg gcacccgcca
     1621 gcctgacacc cagcgctgct tcgccgttcg ccagcgacca atcgagcacc gcccgcaggt
     1681 tctcgtgctc gtcttccagg cgtgcaagcc actcctgttg cctgctaccc ctcagctcgc
     1741 tctcagcagc ctcggtcatc gccaggtaat actcggcatg ccagcggcgt accttatcga
     1801 tctcgctgct ttccaggagg cgctcgcggg cgtactcgcg aatcgtctcc agcatgtaga
     1861 agcgaggttc ttcgttcgcg gccgcataag cttctacgtc acggcccgcc ctgcccagtt
     1921 ttagcaggct cttgtccagc agcgattcca cagcttccag ggtgtcgaat cccaggtcgc
     1981 cctccgcgtt gcacaccgcg tccaccgccg ccagggtgaa ccctccaacg aacacgccca
     2041 gccgcgtaaa gagcttccgc tccccctctt tgagcaggtc gtagctccac gcgatagcgg
     2101 tgcgcagcgt ctggtggcgg tccggtaggt ccctggcacc cttcgccgcc agcattagcg
     2161 accccgcgtc tccgctgccc gcgccgttcg agcctgcggc ggccggtccg gtaagcctgg
     2221 caagcagggc ctggggcgag agcaatttga ccctcgccgc gagcagctca atcgccagcg
     2281 gcagaccatc caggtgcgcg cagatctccg ccacgggcct ggcgttgtgg tgcgtaagct
     2341 cgaaggaaga tacctcctgg gcgcgctcca cgaagagtcc gatggccggg taggcggtga
     2401 gcgcatcgat gggcggaagc gctcccggac cgggcaggcc cagcggctgc agagggaatt
     2461 gctgctcccc tctcaaatgc aacggctccc tgctcgtcac cagcactttc aaggaggcgc
     2521 agtgggcaat cagccgggca accacaccgg ccgcgccgac cacctgctca aaattgtcca
     2581 gcaccagcag catcttcttg tcgcgcaggt gttccaccag ggcggcctca acgctcagcc
     2641 ccggtgactc cttgaggtcg agagtatgag caatcgtgga actgacaagc ccgacgtcgc
     2701 tgataggagc gaggggcaca aagaacacgc cgccagcaaa gtcgggcagc atatcgtggg
     2761 caatctcgac cgccaggcgg gtcttgccga tacccggagg gccgacgatg gtgagcaagc
     2821 gcacgctctc tcgcaacagg cgggcgcgaa tggcagccag gtcctcattg cggcctatca
     2881 gcggagtcgg tgggagagta aggttcgttg gcgtccgtgg gggtgcgggt ggctctgccg
     2941 gttgcggtgg ctgtaccgtc ccgccaggat gtgggggttc ttcgcgtctc tcgagggcgg
     3001 ttatctgctc cccagcagcc ctgccttccc tggccaggct catgaaggcc tcgcgcctgt
     3061 cgggtggaat tcgtagcgca tcggcaagca atactacgac ctgccgggag ggcctccggt
     3121 cccctgtctc gatcttctgg accatccaga ccgagcagcc cacgcgctcc gccagttctt
     3181 cctgcgtgag gtccagcgcc ttacgccgct ccttgagcca ctggccgaat gtcgtctctc
     3241 ccatcacaaa agcccctata gctttagcta tggaccggcc tgtccagtcc cgccatacgt
     3301 gcctgcggcc ctgctgtgaa gcggggtaca ggctcgcatg gccgggacgt gggttgtccc
     3361 cgccctgcaa tgccccgacc atgagcgaga caccgcaaga cgcaggaagc tgctgtgcac
     3421 atttttgtgc aggtttgtgc gacctattgt gcgagccatt atatcctatc ctctgctcta
     3481 atgtaggtag ttacttcggc taccctggcc tgctctacag cgtccggggc tctatccaaa
     3541 tgtgaggcag cgatgaccaa ccaggcacac cggtccgaca aacagcgtga taacacgcag
     3601 catgagcacc aggggaatgg cacaggggaa ccgctcggcc cctcccagtt gttcctggtg
     3661 cgcgtctggg ccgagcaagg tatggaaggc agccctgagt ggcgtggcag agtgcagcac
     3721 gtgaccaggg gagacgccca ggaattcagc gactggggca cgctgataga cctctttgtc
     3781 acgatggtcc ccgcccttga gagcaagtga taccccgttg ggatgcgcgc aaacggcagt
     3841 agctcaatca gattacaagt agcacaggag gttcggacat ggttgctaat ctcggtcagt
     3901 acaaaaggca ggtagtacgt ttcatttcgg cgctgctgtt agtcgtattc ctggcgtcgg
     3961 cgtcctggtc gccggccggg gccgcgagca tggcttctcc gaacgcaagc cctgaggcag
     4021 tcaccgtcca ctgcgtggat gataacggca ccaccacggg taccccctgc gccaacgcga
     4081 ctccctacaa caacatccag gcggcgctcg acgcggcgag tcctggcgat gaggtccgcg
     4141 tggcggtggg cacttacgtg cgcgcggcgg gcaggggcgc tactccgccc aacaacgtga
     4201 tcactatcac gggaggttac gcgggcggtt ccgccgctgg tggttggact gtaaaaggcc
     4261 cggcgaccgg caccgtgctc gtcggcacga acttcaacct gccccccggc gtggtagtta
     4321 ccctccagaa cctcgtcgtc agcaacgcga gcatcgacaa ccagggtggc actctccacg
     4381 tagcgaccgg cgatctgcac ctgcaaggtg ggacgagtaa aggggcgttc gacatagagg
     4441 ccggggccaa agtcgatttc agccagggct ctcacactct cagcaacggc accacctttg
     4501 ccggtagcgg taccgtcagg gtgagcggag gcacattggt tgtgggaagc agcgcgaccg
     4561 agcgtgtcgc ggcgcgccgg gtagaactgg cctccggcac gttgagcggc cccgctaccc
     4621 tcgccataag caacaccctc acgtggaccg gcggcacgat gtcggaagcc ggaaagaccg
     4681 agctggtgcc aggcgctacc ctttcgataa gcggcgcggc ggccaagacc ctcaatgcgc
     4741 gcaccctcaa caaccagggt acggccacct ggctgggcgc gggcgctatc agcctggagg
     4801 cgggggccag catccacaat agcggcctct ttgacgccca gaacaacgcg accctctcag
     4861 cccccggccc cgcggctttc tacaacaccg gcaccttccg caagtcggcc agcaccggcg
     4921 ttacaaccat ccactccaac gtcaagttcg agaacaacgg cggcacggta gatgtagccg
     4981 gtggtacgct gaggttcggt accgcgagcg ttgccggggc ttcctccgaa gtgtccgccc
     5041 ctgaaatcag gtttgacgat cccaacttta tgaattccaa cataagggtc ggtaacactt
     5101 cggcagcttc cagcccacag gcctcgaacg cggtgcttga gttcgcgagt gggacgttcg
     5161 atttccaggg cagcaatact gtcgtgggtg acggcgttgc cagggtcaac ggggcttcgc
     5221 tccgcgtgca gggcaccctg gctgcccgga accttgagct ggcaagcggc ttgattgacg
     5281 gggccggtac cctggccgtg agtggcgcat tcaactggag cggcgggcag atgagcggcg
     5341 cgggggcaac caacatctcg aacggagcct ccctgaatct gagtggcagc aacgtcaaga
     5401 ccctgagcgc ccgtaccctc aacaaccagg gcacggccac ctggctgggc gcgggcgcta
     5461 tcagcctggc agccggtgca accctaaaca acagcggcct gttcgatgcg cagaacaacg
     5521 ccgccatcac cgcccccggt ccggcgacgt tccgcaacac cggcaccctg cgcaaatcgg
     5581 ctagcacggg cgttacagcc atcgactccg ccgtaaagtt cgagaacgac aaaggcaccg
     5641 tggaggtagc cggtggcacg ctgaggttcg gtgccgcaag cactgccgtt gccccctcca
     5701 aggtctccag tcctgacggt gcagccggta cgatttggat cagttcgggg ctgaagatcg
     5761 gtaacacggc agcggcttcc ggcccgcaag cgtcaaacgc cgtagtggag ttcgcgggag
     5821 gagtgcatag cttcgagg
//
LOCUS       DASRWZ010000534         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_866328, whole genome shotgun sequence.
ACCESSION   DASRWZ010000534 DASRWZ010000000
VERSION     DASRWZ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866328"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..1862)
                     /locus_tag="VFH60_08325"
     CDS             complement(357..1862)
                     /locus_tag="VFH60_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313826.1"
                     /translation="MSIARGHAAELLSLGLAAAQSGDRRDWAQAEHYLEWVTLTDAEM
                     DQQAQAWYWLSRVTDDPKRKAECLENALSIQPGHPEARRDKAILDGRLDPTRMRANSA
                     SSAAPTVQAEQIENLQGKRFPCPKCGAVATYDPNVGMLRCQFCGSKLDATGQAVDESA
                     GTSFAGEVSDQDWVSAIYTEKGHAWVLPQQRVLHCQGCGASVTFSPARISASCAYCGS
                     AYVVLELAGADNDLREPDGIVPFTFDAHAGLGYARQWLGEQARLMGVPDDLPRVATLQ
                     LPQPVYLPFWTFDIVGEVRWSGWIRADHDFEELDSLDVVAGLGGVAIGMAIGSFSMVA
                     QSAADTVARRQQNKSNLVHSAGAVGAIVTDVLVPASHSMSPEMLRGIKYHMQYAVRYS
                     PDMLVDWPAEIYSISLADASISARQRAVEESNRQIELESGVEIDGSNRTLTVDRTGLS
                     VQSYKLLLMPVYTTAYTYRDRTFRLVVNGQTGQVLGDVPGANSFVRKILDM"
BASE COUNT          417 a          651 c          698 g          388 t
ORIGIN      
        1 cggccgtgcg cgtgacggag aagaagcggc ccgacgtggc ccaccggggg actcgggctt
       61 cgctaccgag tctgcttaca gcgaagttgc caggcagcag agatagggtc agcgcgattt
      121 gcgacatggc acattataaa ttaggaatgg gtctcgcata aatgccaaca cgcccaatat
      181 caggactaaa tcacccctct gtcatcctga acgcagcaaa gggtccaacg aaggcgagta
      241 agggcgccgt agggaccagg agtagtggtc gggattacag aaagggccgg tgccaatcgt
      301 atggcaccgg cccctcagta cttctcttga acacgttccg tttggaggcg tgtgccttac
      361 atgtccagta tcttgcgcac gaagctgttg gcaccgggca cgtcgcccag cacctggccg
      421 gtctgcccgt tgacgacgag ccggaacgtg cggtcgcggt aggtataggc ggtggtgtag
      481 acaggcatga gtagcagctt gtacgattgg accgacaggc cggtgcggtc cacggtgagc
      541 gtgcggttgg agccgtcgat ttccactccc gactccagtt ctatctgcct gttgctctct
      601 tccaccgcgc gctgccgggc ggagatggaa gcatcggcca gggagatcga atatatctcg
      661 gcgggccaat caacgagcat gtcggggctg tagcgcactg cgtactgcat gtggtacttt
      721 atgccccgca gcatctcagg cgacatagag tggctggcgg gcaccagcac gtcggtcacg
      781 atggccccca ccgcgcccgc ggagtggacc aggttgctct tgttctgctg ccgcctggct
      841 accgtgtcgg cggcgctctg agcgaccatg ctgaagctgc ctatcgccat gccaatcgcc
      901 accccgccga ggcccgccac cacgtccagg ctgtccagct cctcgaaatc gtggtcggcc
      961 cgtatccagc cgctccaacg cacctcgccc acgatgtcga acgtccagaa gggcaggtac
     1021 acgggctggg gcaactgcaa ggtcgccacg cgaggcaggt cgtcgggcac ccccatgagc
     1081 cgggcctgct cgccaagcca ctgccgggcg taaccgaggc cggcatgggc gtcgaaggtg
     1141 aaaggcacga tgccgtccgg ctcgcgcagg tcgttgtcgg ccccggcaag ctcaagcacg
     1201 acgtaagccg agccacaata ggcgcaactg gccgatatgc gagcaggtga gaaggtaacg
     1261 gaggccccgc agccctggca gtggagcacc ctctgctggg gcagcaccca ggcatgcccc
     1321 ttctcggtgt agatggcgga cacccagtct tgatcgctca cctcccccgc gaacgacgta
     1381 ccagccgact cgtccaccgc ctgcccggtg gcgtcgagtt tcgagccgca gaactggcag
     1441 cgcaacatgc cgacgttggg gtcgtaggtg gctacggctc cgcacttggg acaggggaag
     1501 cgcttgcctt gcaggttctc tatctgctcg gcctgcacgg tgggtgcggc gctggacgcg
     1561 gagtttgccc gcatcctggt ggggtcgagt cgcccgtcga ggatagcctt gtcgcgcctg
     1621 gcctccgggt ggcccggctg tatagagagg gcgttttcga ggcactcggc cttgcgcttg
     1681 gggtcgtcgg tgacgcggga gagccagtac caggcttgcg cctgctggtc catttcggcg
     1741 tcggtgagcg tgacccattc caggtagtgc tcggcctgcg cccaatcgcg ccggtcgccc
     1801 gactgcgcgg cggctaaccc caggctcagt aactcggcgg catgccctct tgctatagac
     1861 atatttggcc ctcttgcaga cgatagacga tggatgattg cgggaacgca aacactgctt
     1921 ggagcctagt tacggctaag cggtggaagc ttccacgtat agaatagcgg ttccggggcg
     1981 aactttgaat gctcctagta ctaaattggg agaaaagtac ctcgcggcag gcagcccgaa
     2041 aattgtagcc gctgcgacat agcactgagc agatgtgaaa ccgcaaaggc gcaaagagcg
     2101 caaagggcac aatacggatg gtgtgttgca cgtacaaaaa ggaccaaggt acga
//
LOCUS       DASRWZ010000535         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_317483, whole genome shotgun sequence.
ACCESSION   DASRWZ010000535 DASRWZ010000000
VERSION     DASRWZ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317483"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFH60_08330"
     CDS             <1..362
                     /locus_tag="VFH60_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313827.1"
                     /translation="QRVLFNVTSVWKGPATSQVIVSVANKVCSAIVHDFYKDEGFLVF
                     ANNGALGSLEAPTNSRTQELGKAAEDLQVLGQGKSPAEQAELATLFYLQSYWAEVVLG
                     IVLMLALFGFVRANRAR"
     gene            450..1247
                     /locus_tag="VFH60_08335"
     CDS             450..1247
                     /locus_tag="VFH60_08335"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HET6313828.1"
                     /translation="MSQPIKGVARVVLVASGKGGVGKTTVTVNLALALAAQGARVGLF
                     DADVYGPNVPLMLGVRRTEAADGFIPIGRAQADPYIQPLERFGLKVMSVGLLVGEKQA
                     VMPDPRFVGLVVSRTIKDVVWGELDYLLVDLPPGTGEPQQSLVQAVSVDGVVVVTTPQ
                     DMSLLDAGRSLALYRQAGVPVLGVVENMSYLLCPDCGKRIEVFHRTERDWAVESDELE
                     VLGHIPMSPNVSRGIDAGHPLLSSTPNGTESAVFRQIATRLAERLGS"
     gene            1629..2372
                     /gene="purQ"
                     /locus_tag="VFH60_08340"
     CDS             1629..2372
                     /gene="purQ"
                     /locus_tag="VFH60_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET6313829.1"
                     /translation="MRVGIIVFPGSNGDHDALYSLGEVLGQRAELVWHTATDLARYDL
                     VVLPGGFSYGDHLRAGAIARFAPAMEAVRRYADEGGRVLGICNGFQVLTESHVLPGAL
                     MRNAELAFSCRWVHVRLEPSRSGLLSTAAPGTVWRLPIAHGEGRYFAAPETLDALEAN
                     GQIVLRYCAPDGTLDPLHNPNGSSHGIAGVCNAEGNVLGLMPHPERAAEAILGGDDGR
                     LFLQSVIEAWQGRGAQPAPRNLEAATAAS"
     gene            2374..>3011
                     /locus_tag="VFH60_08345"
     CDS             2374..>3011
                     /locus_tag="VFH60_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313830.1"
                     /translation="MIQRPSTRQPLSEDPVDLERRRLELEYRVEHGRADEPVAHAGGV
                     SALGKLAAALAILVALAYLALWIGSVRNSGGPEGYVRSIDFAAPLTGGYMVRYGDGPD
                     LYDLTSQRVAQDNLLSGAGADTSQFRPYTRLPFEALALAPFLQVPLPLVFAIWALGAG
                     LAIGLSLGMMDGALSVSRGVGWALSLAACSFLPLIRGLMEGQDTPFVLMGLCG"
BASE COUNT          495 a          923 c         1018 g          575 t
ORIGIN      
        1 atcagcgcgt ccttttcaat gtcacgtccg tctggaaagg tcctgcgacc tcccaagtca
       61 ttgttagcgt tgcaaataag gtttgcagcg ccatagtaca cgacttctat aaagatgaag
      121 gctttctagt tttcgctaat aacggcgcac ttggcagctt agaagcgccc acaaatagcc
      181 gcacccagga gctagggaag gccgctgaag accttcaggt cctgggccag ggtaagtccc
      241 ccgccgaaca ggccgaattg gcaacgctgt tctacctaca atcctactgg gccgaagtcg
      301 ttcttggcat tgtgctaatg ttggctcttt tcggtttcgt gcgggctaac agggctagat
      361 agctattctc aggttgatgc aatacaggtt ggcggacccg aaagggtctt gtctgccacc
      421 aaaaatccta aatgctaaat ggaggaacat tgtctcagcc tatcaagggc gtcgcgcgtg
      481 ttgtgctcgt ggccagcggc aagggcgggg tgggtaagac taccgttacc gtgaacctgg
      541 cgctggcgct ggcggctcaa ggggccaggg tggggctgtt cgacgccgac gtttacgggc
      601 cgaacgtgcc gctcatgctg ggggtgcggc gtaccgaggc ggcggacggc ttcatcccca
      661 tcggtcgcgc ccaggcagac ccctatatcc agccgctcga acgcttcggc ttgaaggtga
      721 tgtccgtagg gctactggtg ggggagaagc aggcggtgat gcccgacccg cgcttcgtgg
      781 ggcttgtggt gtcgcgcacg atcaaggacg tggtgtgggg cgagttggac tacttgctgg
      841 tggacctgcc gcccggtacc ggggagccgc aacagagcct ggtccaggcg gtcagcgtgg
      901 acggggtagt ggtggtcacc acgccgcagg acatgtcgct gctggacgcg gggcggtcgc
      961 tggcgctcta ccggcaggcg ggcgtgccgg tgctgggcgt ggtggagaac atgagctacc
     1021 tgctttgccc cgattgcggc aagcggatag aggtgtttca ccgcaccgag cgcgattggg
     1081 cggtagaaag cgacgagctt gaggtgctcg gccacatccc gatgtcgccc aacgtcagcc
     1141 gtggtatcga tgccggccac ccgctcctct cctcgacccc caacggcacc gagtcggcgg
     1201 ttttccggca gatcgcaacc aggctggcgg agcggcttgg gagctaggga ggccaccctt
     1261 cagcctttgc gccggcggag acgcggttcc cctctgccac gacgcggcct gatgaggacc
     1321 aagagtggcc ttgtcatcct gagcgtagcg aaggatcagg tgggtaggcc agagtcttca
     1381 acattgggga cgaaggtggt taggggagca ttgtcgcaac tcaagggaga gtcaagcacg
     1441 ggcggctgat ccttcgctac gctcaggatg acaaaggcac tcatgtgtgc tgtttattgc
     1501 agtgtgttgt tggcacgctc tctggtatca ttgtcgtagg gaaggtgttg ctggagcaga
     1561 ccctacctcg tagatgatcg ttgtaagcgc agccaagtag acgtaaggtt gaggagcata
     1621 tgagcacgat gcgcgtaggt attatcgttt tccccggctc caacggcgac cacgacgccc
     1681 tctactcgct gggagaggtg cttgggcagc gggccgagct tgtctggcac accgccaccg
     1741 acctggcccg ctacgacctc gtcgtgctgc cgggcggctt ttcctacggc gaccatctgc
     1801 gggccggggc tatcgcgcgg ttcgctcccg cgatggaggc ggtgcgccgc tacgccgacg
     1861 aaggaggccg ggtgctcggt atctgcaacg gcttccaggt gctcaccgag tcgcacgtgc
     1921 tgccgggtgc cctcatgcgc aacgccgagc ttgctttctc ctgccgctgg gtgcacgtgc
     1981 gcctcgagcc gtcgcgctcc ggcctgctga gcacggccgc acccggcacc gtctggcgct
     2041 tgcccatcgc gcacggggaa gggcgctact tcgccgctcc tgagacgctg gacgcactcg
     2101 aagccaacgg gcagatagtg ctccgctact gcgcgccgga cggcacgctc gacccgctac
     2161 acaaccccaa cggctcctcg cacggcatag cgggcgtgtg caacgccgag ggtaacgtgc
     2221 tcggcctgat gccccacccg gagcgcgctg ccgaggccat cctgggcggg gacgatggtc
     2281 gcctcttctt gcagtccgta atcgaagcct ggcagggccg gggagcgcaa ccggctccgc
     2341 gcaacctgga ggccgccaca gccgcgtcct aagttgatac aaagacccag cacccgccag
     2401 ccattatcgg aagacccggt tgacctcgaa cgccgccgcc tggagcttga gtacagggtc
     2461 gagcacgggc gtgcggacga gccggtagcg catgcgggcg gggtctcggc gctgggcaag
     2521 ctggcggcgg cccttgctat cctcgtggct ctggcttacc tggcgctctg gatcggctcg
     2581 gtgcgcaaca gcggcgggcc agagggctac gtgcgctcca tcgacttcgc ggccccgctc
     2641 accgggggct acatggtgcg ctacggcgac ggccccgacc tgtacgacct tacctctcag
     2701 cgagtggcgc aagacaacct tctgagcggc gcgggtgccg atacgagcca gtttcgccct
     2761 tacacccgcc tccccttcga ggcgctggcc ctggccccct tcttgcaggt gccgctgccc
     2821 ctggtgttcg ctatctgggc gctgggcgcg gggcttgcca ttggcctgtc gctcggcatg
     2881 atggatggtg cgctatccgt ttcgcggggg gtgggctggg cgctgagcct ggccgcatgc
     2941 tcgttcctgc ccctgatacg cgggctgatg gaggggcagg acaccccgtt cgtgctcatg
     3001 ggcctgtgcg g
//
LOCUS       DASRWZ010000536         6851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1208733, whole genome shotgun sequence.
ACCESSION   DASRWZ010000536 DASRWZ010000000
VERSION     DASRWZ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208733"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1003
                     /gene="sucD"
                     /locus_tag="VFH60_08350"
     CDS             146..1003
                     /gene="sucD"
                     /locus_tag="VFH60_08350"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET6313831.1"
                     /translation="MSILADKNSRVVVQGITGREGSFHTQQMIEYGTGVVAGVTPRKG
                     GTTHLDVPVFDTVKDAVRETGANVSLIFVPAAYAADAMFEAIAADVPLVVCITEGVPV
                     LDMMRVYDVLKESNSRLIGPNCPGLITPGQAKIGIIPGYITMPGNIGLVSRSGTLTYE
                     VIFALTERGMGQTTAVGIGGDPIHGTDFIDCLRLFQDDPDTERIVMMGEIGGDEEERA
                     AAFIKEYVTKPVAGYIAGRTAPPGRRMGHAGAIISGGAGTAQGKIDAMEEAGVKVARI
                     PTQIPDLLA"
     gene            1091..2341
                     /gene="fxlM"
                     /locus_tag="VFH60_08355"
     CDS             1091..2341
                     /gene="fxlM"
                     /locus_tag="VFH60_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase, FxLD system"
                     /protein_id="HET6313832.1"
                     /translation="MQTDVDIDALQSALVDGMRTNGAMRSPAVEAAFRAVPRHLFVPG
                     APLEDVYRDEFIPIKSENARLLSSSSQPSMMAVMLEQLELASGHRVLEIGTGSGYNAA
                     LMSHIVGESGHVVTVDIEEDLIVLARQHLAEAGFGRVEVVCGDGGLGYESGAPYDRVI
                     LTVGAWDIAPAWWDQLRPGGLLVMPLSLRGPQKCIAFRREEDRLVSLSARDCGFIGLR
                     GAFAGPDTIIELGEEPGLRMASERAEQVDASLAYKLLTGPHRDLPVGVSVTLGDFFGS
                     LSLWLALPEEGACSVFAEGDWLASGLVPALLSSPGSKSVYLATLGLYEAGSLCILARA
                     PEVSAANHEPDDTLPFELIVQSYAPDDTLAMRLVAQIRVWDRQGRPPTERLQVVAYRA
                     DAPIPPGEGMVLQKHWTQLVLNHL"
     gene            2377..2913
                     /locus_tag="VFH60_08360"
     CDS             2377..2913
                     /locus_tag="VFH60_08360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6313833.1"
                     /translation="MTIREATIEDVPSIARVHVDSWRTTYKGIVPQRILDQLTYEGRE
                     ELWRRVLSPDNSSFVYVAEEDGQVVGFACGGPAREDEAPNHAGQLYSIYILQEHQGRG
                     IGRRLFEAVVRELVRMGLHSMALWVIADNPACGFYEALGGRQAYARQEQADDVVLDEI
                     GYGWDDIRPLAAGPVQEV"
     gene            2916..3548
                     /locus_tag="VFH60_08365"
     CDS             2916..3548
                     /locus_tag="VFH60_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313834.1"
                     /translation="MPVRDRQRLITDILTTVLGVPPGNPSFSWMTNKQAEADSGEPYV
                     ALRAIFKALRGNAEAHSLKRERPLNCDAFFGGSYNFIFEFDEIQHFSSFRLKTFDFYP
                     QGAKLGYNLERYRDYCKKHMVEANSYRQAKTTDDFPFAGGRTAQRAYFDAVRDLLPPL
                     HGLNPTLRIAEFEVARVVANDKAGQGIIRDMLRNRLPMILPGPDLTEVSI"
     gene            3545..4216
                     /locus_tag="VFH60_08370"
     CDS             3545..4216
                     /locus_tag="VFH60_08370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET6313835.1"
                     /translation="MSNSSPPNKLREIDDYIQDLFPPDDALLHALDNSQRNELPPINV
                     SPNEGRLLYLLARLCGARRILEIGTLGGFSTINLARALPDGGKLITLEYSPKHAEVAR
                     ANIERARLADRVEVRTGAGLDLLPQVEANGEGPFDLFFIDADKDNYPGYLDWCVRLSR
                     PGSVILSDNLVRDGAVIDPPPDNTFAQVIHHYNRTLSTHPRLETVILPIIRESVDGLG
                     ISLVK"
     gene            4277..5869
                     /locus_tag="VFH60_08375"
     CDS             4277..5869
                     /locus_tag="VFH60_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HET6313836.1"
                     /translation="MADITMESRIAEVTVYTDRARVTRRGAVRLQAGEQRVSLDNLPA
                     GVQDDSVRAGGRGAGVRILGVEVIRQFVTEAPEANVAELQKELEALQDQDAALADEDA
                     ALSSQLEFLKSLRESTAATMPRGISTGRSKLEDVQSLVDYLSREWAGTLARRREIARQ
                     RRELARQIAALVGRQAPPYNSIERKQVNVYVEAAAEVEAEIEVTYGVSGASWQPLYDI
                     RLVESKVSLGYLANVTQQTGEDWPAVQLSLSTARPAVSATIPELDPWYLDVPRPRVPL
                     PPMAAQSRARAVDEASGGGATEAYAMPPSMSVAMPPPPVEVAQATVESTGAAITFKVA
                     RPVAIPSDGSPHKTTVTSLDLDSQLDYVSVPRLAEEAYLRAKITNTSPYILLPGQAGI
                     FHEGDFVGSTWLETVVPNQEFEAQLGVDDRIKVERELTTREISKTLIGNTRKNVFGFK
                     ITVSSFLAWPTRVTLYDQFPVSRNEQIKSKLLEVRPAPIEQSELNILKWELAVAPQQK
                     QTILFGFEVDFPRDLAISGLDL"
     gene            6527..>6851
                     /locus_tag="VFH60_08380"
     CDS             6527..>6851
                     /locus_tag="VFH60_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6313837.1"
                     /translation="MDVPPDSPFYSYIQQLACAHVLGGYEDGTFRPNNPVSRGQVAKL
                     ISNAAGYDDAPAGETFADVPSDSLFYAYIERIASRNIVGGYECGRLSEPCDDQHRPYF
                     RPGDNL"
BASE COUNT         1349 a         2222 c         2115 g         1165 t
ORIGIN      
        1 gtctttgcgt tttcgtatcc ttggcgcgtt ctttgcgtcc ttggcgtctt tgcggttccg
       61 tctattgtcc ctcggcaccg aataggcttt gtgttataat ccccgcgcat gattcccata
      121 aatcgatgag gagagggact cgacaatgag catactggca gacaagaaca gtcgcgtggt
      181 ggtgcaaggt atcacggggc gtgaaggctc gtttcacacg cagcagatga tcgagtacgg
      241 caccggcgtc gtggccgggg tgacccctcg caagggcggc accacccacc tggacgtgcc
      301 ggtgttcgac acggtgaagg acgcggtgcg cgagaccggg gccaacgtgt cgctcatctt
      361 cgtgcccgcc gcctacgccg ccgacgccat gttcgaggcc atcgcggccg acgtgcccct
      421 ggtcgtctgc atcaccgagg gcgtgcccgt gctcgacatg atgcgcgtgt acgacgtgct
      481 caaggagagc aacagccgcc tcatcggccc caactgcccc ggcctcatca cccccggcca
      541 ggccaagata ggcatcatac ccggctacat cacgatgccc ggcaacatcg gcctcgtgtc
      601 ccgctccggc accctcacct acgaagtgat cttcgccctt accgagcgcg gcatgggcca
      661 gaccaccgcc gtgggcatcg gcggcgaccc catccacggc accgacttca tcgactgcct
      721 ccgcctcttc caggacgacc cagacaccga gaggatcgtt atgatgggcg agataggcgg
      781 cgacgaggag gagcgggccg ccgccttcat caaggagtac gtcaccaagc cggtggcggg
      841 ctacatcgcg ggccgcaccg cccctcccgg caggcgcatg ggccacgcgg gggccatcat
      901 ctcaggcggc gcgggcacgg cccagggcaa gatagacgcg atggaagagg cgggcgtgaa
      961 ggtcgccagg atacccacgc agatacccga cctgctggca taaccagtaa tgagtgatga
     1021 ggactgagga ctgagtagta gttgctccgt cctctgcttg cttaattaga gcacgttgag
     1081 tggcccgcct atgcagaccg atgtggatat agatgccctg caaagcgccc tggtggacgg
     1141 gatgcggacc aacggggcga tgcgttcccc cgctgtggaa gccgccttcc gcgccgtgcc
     1201 gaggcacctc ttcgtgcccg gcgcgcccct cgaagacgtg taccgggacg agttcatccc
     1261 catcaagagc gagaacgcac gcctgctcag ttcctcctcc cagccctcta tgatggctgt
     1321 catgttggag cagcttgagc tggcgtcggg gcaccgcgtg ctggagatag gcaccggcag
     1381 cggttacaac gccgctctta tgtcgcacat cgtaggcgag agcggccacg tcgtcacggt
     1441 tgacatagag gaagacctta tcgtcctggc gcggcagcat ctcgcagagg ccgggttcgg
     1501 gcgcgtggag gtggtctgtg gggacggcgg gcttggctac gaaagcggcg cgccctacga
     1561 ccgcgtcatc ctgaccgtgg gcgcgtggga catcgcaccc gcctggtggg accagctgcg
     1621 gccgggaggg ctgctggtga tgcccttatc gctgcgcggg ccgcagaagt gtatcgcctt
     1681 caggcgcgag gaggaccgcc tggtaagcct ctcggcccgc gactgcggct ttatcgggct
     1741 gcgtggcgcg ttcgccgggc cggacacgat aatcgagctt ggcgaggagc ctggcctgcg
     1801 catggccagc gagcgcgccg agcaggtaga cgcaagcttg gcttacaagc tcctgacagg
     1861 cccccaccgc gacctgcccg tgggtgtaag cgtcacgctg ggcgactttt tcgggtcgct
     1921 cagcctctgg ctcgccctgc ccgaggaggg cgcttgcagc gtgttcgcgg aaggcgactg
     1981 gctcgcctcc ggcctggtgc ccgcgctcct gagcagcccc ggcagcaagt ccgtgtacct
     2041 ggctaccctg ggcctgtacg aagccggctc gctctgcatc ctggcccgcg ccccggaggt
     2101 cagcgccgct aaccacgagc cggacgacac cctccccttc gagctgatcg tgcaaagcta
     2161 cgccccggac gacacgctcg cgatgcgcct ggtcgcgcag atacgagtgt gggacaggca
     2221 aggccgccca cccacggagc gcctgcaagt cgtagcgtac cgggcggacg cgcccatacc
     2281 accgggcgag ggcatggtcc tccagaaaca ttggacgcaa ttggtgctca accacctgta
     2341 gctctaactc cgcaagcaaa ccacgaggga cgatccatga caataagaga agccacaatc
     2401 gaagacgtgc cgtccatagc ccgcgtgcac gtcgatagct ggcgcaccac ctacaaaggc
     2461 atcgtgccac agcgcatcct cgaccagctc acctacgagg ggcgcgagga actctggcgc
     2521 agggtgctaa gccccgacaa ctcctctttt gtgtacgtgg ccgaagagga cgggcaggtc
     2581 gtgggcttcg cctgtggcgg cccggcgagg gaggacgagg cacctaatca cgcgggccaa
     2641 ctctactcca tatacatcct ccaggagcac cagggccggg gcattggccg ccgcctgttc
     2701 gaggccgtag tgcgggagct tgtgcgtatg ggcttgcact ctatggccct ctgggtgata
     2761 gccgacaacc ccgcctgcgg cttctacgag gcgctgggcg gacgccaggc ctacgcgcgg
     2821 caggagcagg ccgacgacgt ggtgctggac gagataggct acggctggga cgacatacgc
     2881 ccgcttgccg ccggcccggt acaggaggtc taaacgtgcc cgttcgcgac agacagcggc
     2941 tgattacgga catcctcacg accgtgctgg gcgtgcctcc cggcaatcca agcttcagtt
     3001 ggatgaccaa caaacaggca gaggccgata gtggcgaacc atacgttgcc ctgcgagcca
     3061 tcttcaaggc gttgcggggc aatgccgaag cgcactcgct caagagagag cggcccctga
     3121 actgcgatgc ctttttcggc ggcagctaca acttcatctt cgagttcgat gaaattcagc
     3181 atttcagctc cttcaggctc aagacgttcg acttctaccc gcagggtgcg aagctagggt
     3241 ataacctgga gagatacagg gactattgca agaagcatat ggttgaggcg aacagctaca
     3301 ggcaggccaa gaccaccgac gatttcccct tcgcgggcgg tcgcacggca cagcgggcct
     3361 actttgacgc ggtgagggac ttgctgccac cgctccacgg cttgaacccc accctgcgta
     3421 tcgctgagtt cgaggttgcg cgggtagtag cgaatgacaa agcgggacag ggcatcatca
     3481 gggacatgct gcgcaacagg ctacccatga tcctgcctgg accggacctt acggaggtaa
     3541 gcatatgagc aacagttccc caccaaacaa gctgcgcgag atagacgact acatacagga
     3601 cctcttcccg cccgacgacg cgctgctgca cgccctcgac aacagccagc gcaacgagct
     3661 gccccccatc aacgtctcgc ccaacgaagg caggctgctc tacctgctgg cgaggctgtg
     3721 cggcgcgagg cgcatcctgg aaatcggcac gctgggcggc ttcagcacca tcaacctggc
     3781 ccgcgctcta cccgacggtg gcaagctcat cacgcttgag tacagcccca agcacgccga
     3841 agtggcccgc gccaacatcg agcgcgccag gcttgcggac agggtggagg tgcgcaccgg
     3901 cgcgggcctg gacctgctgc cccaggtaga ggccaacggt gaggggccgt tcgacctctt
     3961 cttcatcgac gccgacaagg acaactaccc cggctacctc gactggtgcg tgcggctgtc
     4021 gcgccccggc agcgtcatcc tctccgacaa cctcgtccgc gacggcgcgg tgatcgaccc
     4081 tccgcccgac aacaccttcg cccaggtgat ccaccactac aaccgcaccc tctccacgca
     4141 cccgcgcctc gaaacggtca tactgcccat catacgcgag agtgtggacg ggttgggtat
     4201 ctcactcgta aaatgagcct acagatgggg gaccaccagg gcgacctcaa tttccggaat
     4261 gtaccggggg agcatcatgg cagatatcac aatggaatcg cgcatagctg aggtgaccgt
     4321 gtacaccgac agggcgcgcg tcacccgccg gggcgcggtc cgcctgcaag cgggagagca
     4381 gcgggtgtcg ctcgacaacc tgcccgcagg ggtccaggac gactcggtgc gcgccggagg
     4441 caggggcgcg ggggtgcgca tactgggggt ggaggtcatc cggcagttcg taacggaggc
     4501 ccccgaggca aacgttgccg agttgcagaa agaactggag gcattgcagg accaggacgc
     4561 cgccctggcc gacgaagacg cggcgctgag ctcccagttg gaatttctca agtccctgcg
     4621 cgagtccacg gcggccacca tgccgcgcgg tatctccacc ggccgctcca agctggagga
     4681 cgtgcagtcc ctggtggact atctatcgcg ggagtgggcc ggcaccctcg ccaggcgtcg
     4741 cgagatcgcc cggcagcggc gagagctggc aaggcagata gcggccctgg tgggcagaca
     4801 ggccccgccc tacaactcga tagagcggaa gcaggtcaac gtctacgttg aagctgccgc
     4861 tgaagtcgag gccgagatag aggtgacgta cggcgtcagt ggggcttcct ggcagccgct
     4921 ctacgatatt cggctggtgg agagcaaagt aagcctgggt tacctggcga acgtcaccca
     4981 gcagaccggc gaggactggc ccgccgtgca actatccctg tccaccgctc gcccggctgt
     5041 aagcgccacc atccccgaac tggacccctg gtacctggac gtcccccggc cgcgcgtgcc
     5101 cctgcctcct atggccgcgc aaagccgtgc tcgagctgtg gacgaggcgt cgggaggcgg
     5161 ggccaccgaa gcctatgcca tgccgccgag catgagtgtt gccatgcccc caccgcctgt
     5221 ggaggtcgcc caggccaccg ttgagagcac aggagccgcg atcaccttca aagtagcgcg
     5281 tcccgtggcc atcccctccg acggctcgcc ccacaagacc accgtcacca gcctcgacct
     5341 tgacagccag cttgactacg tttccgtgcc caggctggcc gaggaagcct acctccgggc
     5401 caaaatcacc aatacgtcgc cctacatcct gctgcccggc caggccggca tcttccacga
     5461 aggcgatttc gtaggttcta cctggctgga gacggtggtg ccgaaccagg aattcgaggc
     5521 gcagcttggc gtggacgaca ggataaaggt ggagcgcgag ctaaccacgc gcgaaatatc
     5581 gaagacgctg ataggcaaca ccaggaagaa cgtctttggc ttcaaaatca cggtaagcag
     5641 cttcctcgcg tggcccaccc gtgtcacgct gtacgaccag ttcccggtgt cgcgcaacga
     5701 gcagataaag agcaagctgc tcgaagtcag gccggcaccc atcgagcaga gcgagctaaa
     5761 catcctgaag tgggaactgg ccgtggctcc gcagcaaaaa cagacgatcc tcttcggctt
     5821 cgaggtcgat ttcccgcgcg accttgcgat cagcggcctg gatttgtgat gcccccgtga
     5881 aattcgtgtt tgcggagata ggccggcatg cctgcccctg caccatccta gtaaaaccgg
     5941 aacccactcc aaaagccaaa gtgcggaaaa tgagcaccca aagtccccct ctgtcatttc
     6001 gaacgtagtg agaaatctcg tccttcagca catagttcca cctgtcgtag atgtagagtc
     6061 ccgtgagaac aggggaacaa cggggtgtag aacgagattt ctcactgcgt tcgaaatgac
     6121 agagggaagc atcggtactg atgcgggact ttcttacttt ttgagatagc ttactagagg
     6181 ctacaggcag tgcgcgttag ctccagtatc actttacaaa ggccgcttgg ccctggtaga
     6241 atactagtag ctccgtgcta ctagcataag agtaccttta accgtctgtc ttatatcgct
     6301 caaggggtgc tctttggacc gtccggttac catctattcc agggtaagtc gtatgatggc
     6361 tgctctcctt gtcatgctaa tctgcatgac tacgttgcag actttccctt tttcaggctc
     6421 accggtagca catgcgcagt cctacggacc aggcaagtca gccagcgccc cagagggcca
     6481 ggagccaacg tcttcgaccc cctcgggtac atgcgattcc acctttgtgg acgtgccgcc
     6541 cgactcgcct ttctactcct acatacagca gttggcctgc gcgcacgtac tcggcggcta
     6601 cgaggacggc acgttccgcc ccaacaaccc cgtgtcgcgc ggccaggtgg ccaagctcat
     6661 atcgaacgcg gcgggctacg atgacgcacc ggcgggcgag accttcgccg acgtgccgag
     6721 cgacagcctc ttctatgcct acatagagcg catagcctcc agaaacatcg tgggcggcta
     6781 cgagtgcggc aggctaagcg agccgtgcga cgaccagcac aggccctact tccggcccgg
     6841 cgacaacctc a
//
LOCUS       DASRWZ010000537         8749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_43001,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000537 DASRWZ010000000
VERSION     DASRWZ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43001"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1067..3178
                     /locus_tag="VFH60_08385"
     CDS             1067..3178
                     /locus_tag="VFH60_08385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6313838.1"
                     /translation="MRWVIGIRWTVVVALLLVGLITLIWRLPSETALWHIGLAATVAL
                     LNVCYLVATWQPSFTTTNLGQILRFAQIPVDLLVFTIIVHFSGGVTGPVFVLYFLYIF
                     VGLAILPPSGAYFVAGVAAGLYALLGVLEVLWMQPPQNVGDAPQVHPSPGTYLGYVLT
                     VSATLMIIAYIANYFSGLLTRNEGTIREQLSELNALYAFTRTASTTHISEEIVHNLVN
                     TAMELERATSCSLLLFNDRGESNFVYNKGFTAEQTARLTQMHLHKDDPIFKVLLADGK
                     GVFAPDVDDVPGLRDLIVRPNTRSFYAVPMWRGDYLLGTLNMSFDRRYTMPLSRWNVL
                     GTMTQQAAMAIERIGLFTEAQRAARESASLYQIGLVTTSSLQIDEVMRLIYEQVNRAL
                     HPDTFYIALYDEDLGELRYDIFVEGGEMLLPFKARLDSVGIASWVIRNRRPISVRNWI
                     TEKEQLPFEANMVGTTAQSLISVPLIAKDKIVGVLSVQAQQPDAFETHHLRLLSSIAS
                     QAALALENARLHATVHEQAQRDSLTGAFNHGTFIDKLNRALEAAREDGSAVALVMLDI
                     DKFKTYNDTYGHLVGNDVLRSVVSSILSHVKSTDVVGRWGGEEFAILLPTVSRAQARL
                     VTERIRQTVAHNIMRDMHNRPIPSPTVSQGIAMFPDDAVQMDELIGKADTALYRAKDL
                     GRNYIVEWISISQAEPEPLPV"
     gene            3346..4140
                     /locus_tag="VFH60_08390"
     CDS             3346..4140
                     /locus_tag="VFH60_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HET6313839.1"
                     /translation="MTDNHNGHGSEQLGGERAALRMGKADLHMHTALGDGLASVRQIF
                     EYVEHRTDLDIIAITDHDDIRGAMEALELIEQRKWRFQVIPGTEITTRHGHLLALFVT
                     RDFPMLKTLEWSVQAVYEAGGIVIAPHPLSWLTTSIREKQLLAQHRGDYPWHGIETFN
                     PSYAGRVAHTRVKELNRTVLHLPEFGGSDAHANSMIGMGYTSFPGTTVEDLLQAIKDK
                     TTVAGGEFMTLGDHGKIAAPNMWKSMIVHPTFKVKRALTRRLKERR"
     gene            4147..5388
                     /locus_tag="VFH60_08395"
     CDS             4147..5388
                     /locus_tag="VFH60_08395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313840.1"
                     /translation="MPEPGLNIALVSPYDYASQGGVNEHIRHLSQELRELGHRTKILA
                     PFSTELADQPEEVYALGAVTPVPANGSVARISLSTDLGRTIKAILRTEHFDVIHLHEP
                     LMPTVPWLVLRYSTSPNVGTFHAYGEYSPVYAMGRPILRRFYDRLHGRIAVSQSAFEF
                     VSQYFGGEFEIIPNGVDVQRFSAPREPLHWMRDGRPTVLFVGRFEEPRKGLRWLLQAL
                     PIVEPYFPDLRLVVAGSGDPEDMAEWLPLRDHTRTDRVVYRSPGSALQVEFTGFVAGD
                     DLPRYYQSCDVYCAPSTGGESFGIVLLEAMAAGTPVLASRIPGYANVLTHGREGFLAE
                     PKNPASIAAGLVRLLSDGELRGRIREAGQRTARQYDWPMIASRIVSHYERTILAHQAA
                     GSTPALPRRRRRRTLQEIIGN"
     gene            5520..6164
                     /locus_tag="VFH60_08400"
     CDS             5520..6164
                     /locus_tag="VFH60_08400"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6313841.1"
                     /translation="MLTQLLQDWARRVARGAARVFVKTPITPNMLTLFGLVLNGIVAI
                     LLATGHLVAGGVMMIFAGLFDMLDGALAKITERVSDFGAFLDSVVDRYSEAIVLLGLL
                     LYYYSLPPSVAGVVNRYFAPLDPKLINIVLIYAILFGSMMISYARARAGALKIKNEVG
                     ILARPERLVLLAVGLLFQTVLLEPVLWLLAIGTQITAVQRIVHVWQVTSGKKQG"
     gene            complement(6459..7238)
                     /locus_tag="VFH60_08405"
     CDS             complement(6459..7238)
                     /locus_tag="VFH60_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313842.1"
                     /translation="MSDSVSDGSLHPGDFSSKPARPAHPRVEPIPVLDAAEQVTVQID
                     LLQEQYEAIRRLSQANGWKEQEGLLTVLMSGVGYLDASLQVEGINRSEDEGELARRVD
                     SLVQDLASYHSMYAVMKFKAFKMYKLNQTLEFNVSGLRATERMWEEWAERMRREQTEL
                     RTEVLRLRSVMSEFDVRTRTDDGGSEVRKFDEVLPFIVPALQHPAAPADDYSAWEQGN
                     HPAPADESSHSADTVRLLPSTQEVRPGLWSRVVQRCKGWFR"
     gene            complement(7414..8733)
                     /gene="rlmN"
                     /locus_tag="VFH60_08410"
     CDS             complement(7414..8733)
                     /gene="rlmN"
                     /locus_tag="VFH60_08410"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET6313843.1"
                     /translation="MEQTSTASQPLTPKPRDPRPNIYDLSLDRLTAVMVEWGEPAFRA
                     KQVLHWLYKELVTGFDAMTNLPQALRRRLEETYRLGSAELVAQKTSTDGWTRKVLLKM
                     KDGSTVESVLMLYYDRATVCVSSQVGCAMGCSFCATGQMGFTRNLTSGEILEQVLWFN
                     RWLREHPHRAVLSNDARPRYTGDKQPQGVNVRSTDAGRNEELNLWHVNSDEHSQKAPN
                     EISAVTNIVFMGMGEPLVNYANLWGAIRMLNSPQGLGLGARRMTVSTVGVVPQIRRMA
                     HEEIAVNLAVSLHASNDELRSQMVPMNTRFNIEELVSACREYIEATKRRITFEYVLIK
                     DVNDGLHLARELAGLLKGMLCHVNLIPLNPVPGTDMLATPREQVFAFQKIVQDAGIWT
                     TVRIERGQDIAAACGQLKVQEEPNSHRRSLNPDLVQAHVQADLAAAM"
BASE COUNT         1637 a         2910 c         2548 g         1654 t
ORIGIN      
        1 agggccagca gccgggcagt accagtaaga aggcgatgac gagcgcggcg gcccgtgcta
       61 aacgcgtaga caaagctact cctaaactcc aggtcttgcg gcggtcggaa gggagacata
      121 acctctatgg gctaggccaa ttgtagcact ccgcgcctgc ttcggcaagc aaacccttac
      181 gctgtccaac gcatgcgtgg ctgattcagc atctaggcaa gcctggcgtt tatacgcaca
      241 gacgccctgt ggggggcgcc ctctggggga cgccctgtgg ctaatgaaga ttaacaactt
      301 gataaggtaa tagcctgtag gggcaggccc ccgtgcctgc cctggtggca ggcgtacaat
      361 gttgctaatc tccaacaagg gcaggcacgg gggcctgccc ctacgcagca cccattcgtg
      421 cctggccatt acctgattaa ccagttaatc ttcataagcc acgggctggg aagaagcgaa
      481 gtctccttcg gagactctct tgcacgaggg tagcatctat ctgcaaagtg ttaggaagca
      541 agcgaacaaa ggtcgccttg tcattctgag cgtagcgaag aatctcgtcc tccaccggga
      601 tgtggccctt ttccttagca catggccaca gatgacacct tgccagcgta aaggaaaggg
      661 ctatttgggt gctgaaggac gagattcttc gctacgctca gaatgacaag ggggaggttg
      721 gtcccacacg agcggcgctc tgtagatacg taccagtctc cgaaggagac ttcgctgctt
      781 cccagcccgt ggcttcagcc acagggcgtc acacgggggg aacgcgacag gccacaccgc
      841 tcgcacccgc acgacacctg cacctctacc tgcacctcga taggtacccc ccggagtagt
      901 actttagtac ttacatcggg cggttgactt aggactctca gttgggtaat atagtcccca
      961 taggctgagt gttttactca ctggatgatg tcgtttgatc tctgttttta ctagaccccg
     1021 gattggcgtt gccggagata taggctctag cgaattggtc gagcggatgc gatgggttat
     1081 cggtatccgc tggactgtcg tggtcgccct cctgctcgtc ggcctcatta cgctcatctg
     1141 gcggctcccc tctgagaccg ccctctggca catcggcctc gccgccacgg tggccctgct
     1201 caacgtctgc tacctcgtcg ccacctggca gcccagcttt acgaccacca acctgggcca
     1261 gattctccgc ttcgcccaga tcccggtaga cctgctcgtc ttcaccatta tcgtgcactt
     1321 ctccggcggc gttaccggcc ccgtgttcgt gctttacttc ctctacatct tcgtcggcct
     1381 tgccatactg ccaccttccg gggcttattt cgtggccggg gtcgccgccg ggctgtacgc
     1441 gctgctgggg gtgctcgaag tcctctggat gcagccgccg cagaacgtcg gagacgcccc
     1501 ccaggtccac ccctcgccag gcacttacct cggctacgtg ctcacagtct cggccacatt
     1561 gatgataatc gcctacatcg ccaactactt ctccgggctg ctcacccgca acgagggcac
     1621 tatccgcgag cagttgagcg aattgaacgc cctctacgca ttcacccgca ccgcctctac
     1681 tacccacatc agcgaagaga tagtgcacaa cctcgtgaat accgcgatgg agcttgagcg
     1741 ggccacgtcg tgctccttgc tcctgttcaa cgaccggggc gaaagcaact tcgtctacaa
     1801 caagggcttc actgccgaac agacggccag gctcacccag atgcacttgc acaaggacga
     1861 tcctatcttc aaggtgctgc tggccgacgg caagggggtc ttcgcgcccg acgtggacga
     1921 cgtgcccggc ctgcgcgact tgatcgtgcg acccaacacc cgctccttct acgcggtgcc
     1981 catgtggcgc ggcgactacc tgctaggcac cctcaacatg agcttcgacc gcaggtacac
     2041 gatgcccctg agccgctgga acgtgctcgg cacgatgacc cagcaggccg cgatggctat
     2101 cgagcgcatc ggcctgttca ccgaggccca gcgcgcggcc cgcgaaagcg cctccctcta
     2161 ccagatcggc cttgttacca cttcctcgtt gcagattgac gaggtgatgc gcctgattta
     2221 cgagcaggtg aaccgagccc tccaccccga caccttctac atcgccctct acgacgagga
     2281 cctgggcgag ctgcgctacg acatcttcgt ggagggtggc gagatgctgc tgcccttcaa
     2341 ggcgcgcctc gatagcgtgg gcatcgccag ctgggtaatc cgcaaccgca ggcccatctc
     2401 cgtgcgcaac tggatcaccg agaaggagca gttgccgttc gaggccaaca tggtgggcac
     2461 caccgcgcag tcgctcatca gcgtgcccct catcgccaag gacaagatcg tgggtgtgct
     2521 ctcggtgcag gcccagcagc ccgacgcctt cgagacgcac cacctgcgcc tgctaagctc
     2581 tatcgccagc caggccgccc tcgcgcttga gaacgctcgc ctgcacgcga cagtgcacga
     2641 gcaggcccag cgcgactcgc tcaccggggc cttcaaccac ggcacgttca tcgacaagct
     2701 gaaccgcgct cttgaggccg cccgcgagga tggcagcgcc gtggccctcg taatgctcga
     2761 catcgacaag ttcaagacct acaacgacac ctacggccac ctggtaggca acgacgtgct
     2821 gcgctcggtc gtctcctcta ttctgagcca cgtcaagagc accgacgtgg tgggtcgctg
     2881 gggtggcgag gagttcgcta tcttgctgcc caccgtgagc cgcgcccagg cccgcctcgt
     2941 gaccgagcgc atccgccaga cggtggccca caatatcatg cgcgacatgc acaaccgccc
     3001 catcccctcc cccacggtga gccagggcat cgccatgttc cccgacgacg cagtgcagat
     3061 ggatgagttg atcggcaagg ccgacaccgc cctctaccgc gctaaagacc tgggccgcaa
     3121 ctacatcgtc gagtggattt ccatcagcca ggccgaaccc gaacccctgc ccgtctgaga
     3181 cggaattacg aattatggca agcatcctgt aattcgtaat tcgtaattgt cttcgtggtg
     3241 acttataatt accgcgcgtc cctgttccgc aactagggtg tgacgcggca gagacaatcc
     3301 tgtgctctgt ctatctccgc gcctgggcgc acgacaggac tactaatgac cgacaaccac
     3361 aacgggcacg ggagcgagca attgggcggg gaacgcgcag cactgcgcat gggcaaggcc
     3421 gaccttcaca tgcataccgc gcttggcgac gggctggcga gcgtgcggca gatattcgag
     3481 tacgtcgagc accgcaccga cctggacatc atcgccatca ccgaccacga cgacatccgg
     3541 ggcgcgatgg aggcgctgga gcttatcgag cagcgcaagt ggcgcttcca ggtcataccc
     3601 ggcaccgaga ttaccacccg ccacgggcat ttgctcgccc tcttcgtcac ccgcgacttc
     3661 ccgatgctca agacgctcga atggagcgtg caggccgtct acgaggcggg cggcatcgtg
     3721 atagctcccc accccctctc ctggcttacc acctccattc gcgagaagca actgctggcc
     3781 cagcaccgtg gcgactaccc ctggcacggc atcgagacgt tcaacccttc gtacgccggg
     3841 cgcgtggccc acactcgcgt caaggaactc aaccgcaccg tgctccacct gcccgagttc
     3901 ggcggctccg atgcgcacgc caactccatg attggcatgg gctacacctc cttccccggc
     3961 acgactgtag aggacctgtt gcaggccatc aaggacaaga cgacggtggc gggcggggag
     4021 ttcatgacgc tgggcgacca cggcaaaatc gccgcgccca acatgtggaa gagcatgatc
     4081 gtgcacccca ccttcaaggt caagcgcgcc cttacccgcc gcctgaagga gcggaggtaa
     4141 gcggccatgc ctgagccggg cttgaacatc gccctcgtct caccctacga ttacgccagc
     4201 cagggcgggg tcaacgagca catcaggcac ctctcgcagg agctaaggga gctggggcac
     4261 cgcaccaaga tactggcccc gttctcgacc gagctggccg accagccgga agaagtgtac
     4321 gccctgggcg cggtgacccc cgtgcccgct aacggctcgg tggcccgcat ctccctctcc
     4381 accgacctgg gccgcaccat aaaagctatc ttgcgcaccg agcacttcga cgtgattcac
     4441 ctgcacgagc cgctcatgcc caccgtgccc tggctggtgc tgcgctattc caccagccct
     4501 aacgtgggca cgttccacgc ttacggcgag tactccccgg tgtacgcgat gggcaggccc
     4561 atcctgcgcc gcttctacga caggctgcac gggcgcatag ccgtctccca gtcggcgttc
     4621 gagttcgtgt cgcagtactt cggcggcgag ttcgagatca tccccaacgg ggtggacgtg
     4681 cagcggttca gcgccccgcg cgagcctctg cactggatgc gggacggcag gcccaccgtg
     4741 ctgttcgtag gtcgcttcga ggagccgcgt aagggcctgc gttggctctt gcaggctctg
     4801 cccatagtgg agccatattt ccccgacctg cgcctcgtgg tggcgggcag cggcgacccc
     4861 gaagacatgg ccgagtggct cccgttgcgc gatcacaccc gcaccgaccg cgtggtctac
     4921 cgctcgcccg gctccgcgct ccaggtcgaa ttcaccggct tcgtagcggg cgacgacctg
     4981 cctcgctact accagtcgtg cgacgtgtac tgcgcgccct ccacgggcgg ggagagcttc
     5041 ggcatagtgc tgctcgaggc gatggccgcc ggtacccccg tgctcgccag ccgcatcccc
     5101 ggctacgcca acgtgctcac ccacggccgc gagggcttcc tcgccgaacc caagaacccc
     5161 gcctccatag ctgccggcct cgtacgcctg cttagcgacg gcgagttgcg gggccgaata
     5221 agagaagcgg gccagcgcac cgcccgccag tacgactggc ccatgatagc ctcccgcatc
     5281 gtctcccact acgaacgcac catcctggcc caccaggccg ccggaagcac acccgccctc
     5341 ccccgcaggc gcagacgccg caccctgcaa gagataatcg ggaattagaa tttcgaattt
     5401 cgaattttga aggtatccga gcaaattcga aatccgaaat cccaatttcc aaatcccgta
     5461 tggtataatt tcccttcgcg tgagtctcca cgctcgcaag aaaccaccgg aggaaggcat
     5521 tgctgacgca gttgctgcaa gactgggcgc gacgagtggc ccggggagca gcgcgagttt
     5581 tcgtaaagac acctattacc cccaacatgc tcaccctgtt cggccttgtg ctgaacggga
     5641 ttgtggctat cttgctggca accgggcacc tggtggcggg cggggtgatg atgattttcg
     5701 cggggctgtt cgacatgctg gacggtgccc tggccaaaat cacggagagg gtgagcgact
     5761 tcggggcgtt cctcgactcg gtggtggacc gctattccga ggcgatagtg ctgctcggcc
     5821 tgctgctcta ctactacagc ctgccgccct ccgtcgcagg ggtcgtcaat cgctactttg
     5881 ctccgctcga ccccaagctc atcaatatcg tgctcatcta cgccatcctg ttcggctcga
     5941 tgatgatcag ctacgcgcga gcgcgagccg gtgcgctcaa gatcaagaac gaggttggca
     6001 tactggcccg cccggagcgg ctggtgctgc tagccgtcgg cctgctcttc cagacggtgc
     6061 tgctggagcc ggtgctgtgg ctgctggcaa tcggcacgca gataaccgcc gtgcagcgca
     6121 tcgtccacgt ctggcaggtt acaagcggca agaaacaagg ctaggtcaat aatccatcag
     6181 gcacagcagg gtctccagcc gacgcccact attaaaatcg agggctacac gttacaaagt
     6241 ctgccgcagg gcagactggt acatcttaga gcatctaaat agaccttgta ctatgcctca
     6301 gtccccttgt gggacttcgc aatgcgtagc cctcgatttc aatcgtgggc gtcggctggg
     6361 gacaacgcgg gaaccccggc attaggcagg tagttaaacg agcaaagccg gatccgtagt
     6421 atggacccgg cttctttatg gtcaacccct atcccgcgct agcggaacca ccccttgcac
     6481 cgctgcacca ctctagacca gaggccgggc cgcacttcct gggtagaggg caggagccgc
     6541 accgtgtcgg cgctgtggct gctttcatcg gcgggggcgg ggtggttgcc ctgttcccag
     6601 gccgagtagt cgtcggcggg ggcggcgggg tgctgcaatg cgggtacgat gaacggcagc
     6661 acctcatcaa acttgcggac ttcgctgccg ccgtcgtcgg tcctggtgcg cacgtcgaac
     6721 tccgacatga ccgaccgcag ccgcaggacc tccgttcgca actccgtttg ctcgcggcgc
     6781 atgcgctcgg cccactcctc ccacattcgc tcggtggccc gcagcccgga cacgttgaac
     6841 tcaagcgtct ggttcagctt gtacatcttg aaagccttga acttcatcac ggcgtacatc
     6901 gagtggtagc tggcaaggtc ctgcaccagg ctgtccacgc gccgggcaag ctccccctcg
     6961 tcctcgctgc ggttgatgcc ctcgacctgc aagctggcat ccaggtagcc tacccccgac
     7021 atcagcacag tgagcagccc ctcctgctcc ttccaaccgt tcgcctgtga gagcctgcgg
     7081 atcgcctcgt actgctcctg cagcaggtca atctgcaccg tcacctgctc ggcggcgtcg
     7141 agtacgggta taggctcgac gcgcgggtga gccggtcttg cgggcttgga ggagaaatcg
     7201 cctgggtgga ggctgccgtc ggaaacgctg tcagacatac tgtgcccctg tacgactcta
     7261 aagcatggtc ggagcgcgga ggacaaggcc ctcctcccgc cccactatat ctactctcgc
     7321 gcgccctcga cggtttaact tacttactgc gcgtcagtag ttattagtca gtgctaagcc
     7381 gccctattct gaccactgac cactaaccac caactacatc gcggccgcga gatcggcctg
     7441 aacgtgcgcc tgcacaagat ccgggttcaa ggaacgccgg tgcgagttgg gttcctcctg
     7501 caccttgagc tgcccacagg ccgccgcgat gtcctgcccg cgctcgatgc gcaccgtggt
     7561 ccagataccg gcgtcctgca ctatcttctg gaacgcgaac acctgctcgc gtggggtagc
     7621 cagcatgtcg gtgcccggta cggggttgag gggtatcagg ttcacgtggc acagcatacc
     7681 cttcaacagg cccgccaact ctcgcgcaag atgcaggcca tcgtttacgt ctttgatgag
     7741 cacatattcg aacgtaatgc gccgctttgt ggcctcgatg tactcccggc acgcagacac
     7801 caactcctcg atgttgaagc gagtgttcat cggcaccatt tggctgcgta gctcgtcgtt
     7861 cgacgcgtgg agggacaccg ccaggttgac ggcgatctcc tcgtgggcca tacgccgtat
     7921 ctgcggcacc acgcccaccg tgctgaccgt catccgcctc gcccccaggc ccaggccctg
     7981 aggggagttg agcatacgga tagcccccca caggttggca tagttgacca gcggctcccc
     8041 catgcccatg aagacgatgt tggtaaccgc cgagatctcg ttgggggctt tctgggagtg
     8101 ctcgtccgag ttcacgtgcc acaggttgag ttcttcattc cgcccggcgt cggtcgagcg
     8161 gacgtttacc ccctgaggtt gcttgtcgcc ggtgtagcga gggcgggcgt cgttagagag
     8221 cacagcgcga tgggggtgct cgcggagcca gcggttgaac cagagcacct gctccaggat
     8281 ctcgccactc gtcaggttgc gggtgaagcc catctgcccc gtggcgcaga aggagcagcc
     8341 catcgcgcag cccacctgcg acgacacgca cacggtggcc cggtcgtaat agagcatcag
     8401 caccgactcg accgtgctgc cgtccttcat cttgagcagc accttgcggg tccagccatc
     8461 cgtggaggtc ttctgggcca caagctccgc cgaacccagg cgatatgtct cctccaaccg
     8521 gcggcgcaga gcctggggca ggttggtcat ggcatcgaag ccggtgacta gctccttgta
     8581 gagccagtgc agcacttgct tggcccggaa cgcaggctcg ccccactcga ccatgacggc
     8641 ggtcaggcgg tcgaggctga ggtcgtatat gttgggcctg ggatcgcgcg gtttgggcgt
     8701 gagaggctgc gacgctgtgc tggtttgctc cataccctat tataaccaa
//
LOCUS       DASRWZ010000538         6359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_660345, whole genome shotgun sequence.
ACCESSION   DASRWZ010000538 DASRWZ010000000
VERSION     DASRWZ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660345"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..2355)
                     /locus_tag="VFH60_08415"
     CDS             complement(190..2355)
                     /locus_tag="VFH60_08415"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313844.1"
                     /translation="MISSSPAGGQKNTGARLALVLLLVALVAGGLLLLAFQYPTGRPS
                     VAPSPTPAAATGTPQLSGTQTQTQTQSPTHSPTHLPASPTSAPPASHTPLPARTSTVA
                     AGSVTPTFTPAALSAAERTLRVLDAEQVPVRDLYSITLRLRPPSDVPLSRIVDSPPAN
                     YPVGHTETFFMSDIVARRYYTITATLRNVTEHVHWYVQDGQQVDDDALDNATREFEDK
                     IYPANHRLFGTEWVPGVDNDPRMTVLIANIPGAGGYYVSADEYTQAVNPFSNQREIIY
                     VNTSGDASGLGSTLAHEFQHMIHWHQNPGHDVWLNEGASMLAQDVNGYGTGGVEHDFV
                     SRPDVQLNAWQASPDASRANYGASFLFFEFLRSHYGGDDVLRAVVAAEGRGADAVDNA
                     LASLGSQDRFLDVFKRWTLANLLDGEGDAQEQGFDYPGRDIGASPQHLVSDYPRSLAD
                     TVSQFGTDYVQLSPPEAGDTLQIDFTGQAETRPVPAPAHSGEGIWWSNRGDQADSSLT
                     RRFDLRSVSRATLHFSAWFRIEDDLDYAYVEASSDNGVTWATLQGDYTTTSNPNGNNL
                     GHAYTGDSAAKPGAGADGWLQEQIDLTQYAGKEVLLRFEYVTDDGFNTEGMAVDDISI
                     PEIGYQDDAEAESDWDGEGFARIANKLPQTYHLAVVKYKDSGFDIQEVEVGQDGKASF
                     EIEGLGAGGPYTEAVLVISGTARHSISPAGYQLNIRVKR"
     gene            2538..4004
                     /gene="proS"
                     /locus_tag="VFH60_08420"
     CDS             2538..4004
                     /gene="proS"
                     /locus_tag="VFH60_08420"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET6313845.1"
                     /translation="MCAKAEQKEFGQGIADQETDFPQWYIDVVTKAELAEDAPVRGCM
                     VVRPYGYALWENCQANLDRRFKDTGVVNAAFPLLIPQSFLLKEAEHVEGFAPEVAWVT
                     HGGGEELAEPLAVRPTSEAIIGPIYSRWIQSYRDLPLLINQWCSVFRWEKRPRLFLRT
                     AEFWWQEGHTVHANHDDCEERARLMLDVYRAFLEEELAIPVVPGQKSEGQKFPGALRT
                     YTVEAMMQDGRALQSGTSHNLGDHFARAFDIMFLNEQNERVHGWTTSWGMSTRVIGAL
                     IMVHGDKKGLVLPPRIAPHQVVVLPLWRKDEEKAGVMAAVDRVVGTLKGAGVRVKVDS
                     DDSKTVGWKHNEWELRGVPLRMAIGPRDVENGTVELARRDKPGKEFKEYPSIDSLAEC
                     VPQLLAEIQQNLYQRALEFRVRNTRHVADMEELAQAIEERQFADAFWCGMSECENYIK
                     EAVKATNRCMPLDQPGDSGPCVVCAMGSKVHAIFARAY"
     gene            4198..4563
                     /locus_tag="VFH60_08425"
     CDS             4198..4563
                     /locus_tag="VFH60_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313846.1"
                     /translation="MQCEWRITGPEGLIVGLNDRYFPAGDAFDKPPDFNWDVQGANRL
                     DERLSRFMEQRRSRPLVVEQVWADHVGSFRLLFDGGYSLDVFPFETTKYEYWRLFKPG
                     LPDAGEVPHFVVTGEGLED"
     gene            4560..5717
                     /locus_tag="VFH60_08430"
     CDS             4560..5717
                     /locus_tag="VFH60_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313847.1"
                     /translation="MSARQPATRVNSQGEKAAYLSLLLIATLVLFAGLLPRFPSTATT
                     LPTPTPTTQTKIQNPKSKIGLHTRLTDESDAEKIRQEFRMLREMGGSYAVEFFPWAYI
                     QAHDRNRFDWEHADLVVDAAREYGITLIARVDGVPNWAKPENATWRYLDREHYDDYAA
                     FVAAFVQHFKGRIGHYIIWNEPNTAAEWGQRPPDPEGYAELLKVVYTRAKQADPSAVI
                     MLAGLAPNTEHEGSPNAMSDLAYLDRLYRAGAAPYFDAVAVHSYGLTAPPGDPASPGK
                     VNWARTEEIYRVMVQHGDGDTPVYITEGGWNDSPRWSFAVKPYQRVEYTVEAYRMAEE
                     QWPWVRAVCLWASRFPRPQYTYFDNYSFLTPDFIPKPVYLEVQQYAERGGR"
     gene            5825..>6359
                     /locus_tag="VFH60_08435"
     CDS             5825..>6359
                     /locus_tag="VFH60_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6313848.1"
                     /translation="MPRNLLNIAVVLALLAVSALVWLGQQKPPDRFLERIRQTGTLRV
                     GMDPTYPPFDVVQDGQVRGYDAALAQAIARDLGVQVEFKTLALDTLYDALIAGNVDML
                     VSALPPIPERQADVRYSVPYYQSGQVLVVRSGEKAIIGIGGLAGKKVGVELGSNADTE
                     ARRLQRSTAPGMQLSSVY"
BASE COUNT         1150 a         1990 c         2106 g         1113 t
ORIGIN      
        1 ggactcctca agccccatat tcacttgatc gaggcacaaa cgccctcaac cagtctgccg
       61 cagggcagac ttcgtaatgt gtagcccgcg atttcaatcg ccgggcgtcg gctggcacca
      121 tgtgtggctt gtatatgcag aagtggcagg gcgtctccca gggccgaggc aaagttgcaa
      181 gggcaaagcc taacgcttca ccctgatgtt tagctggtac cccgcgggcg agatgctgtg
      241 ccgcgccgtg cccgatatta ccagcaccgc ctccgtgtaa gggccacccg cgcccagtcc
      301 ctctatctcg aagctggcct taccatcctg cccgacctct acttcctgta tgtcgaagcc
      361 gctgtccttg tacttcacca cggccaggtg gtaggtctgg ggcagcttgt tggctatgcg
      421 tgcgaagccc tcgccgtccc agtccgattc ggcttcggcg tcgtcctggt agccgatttc
      481 ggggatagag atgtcgtcca cggccatgcc ctcggtgttg aagccgtcgt cggtgacgta
      541 ctcgaagcgg agcaggactt ccttgcccgc gtactgcgta aggtcgatct gctcttgcag
      601 ccagccgtcg gcaccggcac cgggtttggc ggcgctgtcg ccggtgtagg cgtggccgag
      661 gttattgccg ttggggttgg aggttgtggt gtagtcgccc tggagagtgg cccaggtgac
      721 gccgttgtcg gaagaggcct ccacgtaggc gtagtcgagg tcgtcctcga tgcggaacca
      781 ggcgctgaaa tggagggtgg cgcggctgac gctccgcagg tcgaagcggc gggtaaggga
      841 gctatcagcc tggtcgcccc ggttgctcca ccagatgccc tcgccgctgt gcgcgggagc
      901 gggcaccggc cgggtctcgg cttgccctgt gaaatcgatt tgcagggtgt ccccagcctc
      961 cgggggagac aactgcacgt aatcggtgcc aaattgcgac actgtatcgg ccagcgacct
     1021 ggggtaatcg ctcaccaaat gctgcggcga cgccccaata tcacgccccg ggtagtcgaa
     1081 gccctgctcc tgcgcgtccc cttcgccgtc cagcaggttc gccagcgtcc accgcttgaa
     1141 tacgtcgagg aacctgtctt gagagccgag gcttgcgagc gcgttgtcta cggcgtccgc
     1201 gccccggccc tcggcggcca ccaccgcccg cagcacgtcg tcgccgccgt agtgagagcg
     1261 caggaactcg aagaagagga aggaggcccc gtagttggcg cggctggcgt cggggctggc
     1321 ctgccaggcg tttagctgca cgtcgggccg ggacacgaaa tcgtgttcca cgccgccggt
     1381 gccgtagccg tttacatcct gcgccagcat ggaggcacct tcgttgagcc acacgtcgtg
     1441 gccggggttc tggtgccagt ggatcatgtg ctggaactcg tgggcgagcg tgctgcccag
     1501 cccggaggca tcgccgctcg tgttgacgta gataatctcg cgctggttgc tgaaggggtt
     1561 gacggcctgg gtgtactcgt ccgccgacac gtagtagccg ccagcgccgg gaatattggc
     1621 gatcagcacc gtcatgcgag ggtcgttgtc cacgccgggc acccactcgg tgccgaacag
     1681 ccggtggttc gccgggtata ttttgtcctc gaattcccgc gtggcgttat ccagcgcgtc
     1741 gtcgtctacc tgctgcccat cctggacgta ccagtgcacg tgctcggtca cgttgcgcag
     1801 ggtagcggta atggtgtagt agcgcctggc gacgatatcg ctcatgaaga aggtctcggt
     1861 atgtcccaca gggtagttgg caggagggct gtccacgatg cgggacagcg gcacatccga
     1921 tgggggtctg agccgcaggg tgatcgagta gaggtcgcgc accggcacct gctcagcatc
     1981 cagcacgcgc aacgtccgct cggcggcgga aagagcggcg ggcgtgaagg tgggcgtgac
     2041 gctgccggca gctacagtgc tcgtgcgagc gggcagcggg gtatggctag ctggtggggc
     2101 gctcgtgggc gaggcgggga ggtgggttgg cgaatgggta gggctttggg tctgggtctg
     2161 cgtttgcgtg cccgaaagct gcggagtgcc tgtagcggcg gcgggggtag gggacggggc
     2221 aacgctgggc cgccccgtgg ggtactggaa agccagcaac agcaagcctc ccgctaccag
     2281 tgctaccagg agcagcacca gcgcgagccg cgcgccggta ttcttttgcc cgcctgcggg
     2341 ggaggaagat atcaaatagt ggctgccttg ttcgggacgt tatcgagccg cgtcacgctt
     2401 cagcttatta tcccacagcc ggcataggat gcaaacccaa ccgcacgatc tgtattctcg
     2461 cccctgattg acgctgcatg gcacttttgc tatagttcct ggaactaacc caactagaac
     2521 gaggtacaga ggtacttatg tgcgccaaag ctgagcagaa ggaattcggg cagggcatag
     2581 ccgaccagga gactgatttc ccccagtggt acatcgacgt ggtcacgaag gcggagcttg
     2641 cggaagacgc cccggtgcgc ggctgcatgg tcgtgaggcc ctacgggtac gccctctggg
     2701 agaactgcca ggccaacctg gaccgccgct tcaaggacac gggcgtggtc aacgcggcct
     2761 tcccgctgct catcccgcag agcttcctgc tcaaagaggc cgagcacgta gagggtttcg
     2821 cccccgaagt ggcctgggtc acgcacggcg gcggcgagga gcttgccgag ccgctggcgg
     2881 tgcgccccac ctccgaggcc atcatcggcc ccatctactc gcgctggata cagtcgtacc
     2941 gcgacctgcc cctcctcatc aaccagtggt gctccgtctt ccgctgggag aagcggcccc
     3001 gcctcttcct gcgcacggcc gagttctggt ggcaggaagg ccacaccgtg cacgccaacc
     3061 acgacgactg cgaagagcgc gcgcgcctca tgctggacgt ttatcgcgcc ttcctggagg
     3121 aagagcttgc catcccggtg gtccccggcc agaagagcga gggccagaag ttccccggcg
     3181 cgctccgcac ctacacggtc gaggccatga tgcaggacgg ccgggcgctg caatcgggca
     3241 cctcgcacaa cctgggcgac cacttcgccc gcgccttcga catcatgttc ctcaacgagc
     3301 agaacgagcg cgtccacggc tggaccactt cctggggcat gtctacccgc gtcataggcg
     3361 cgctcatcat ggtgcacggc gacaagaagg gcctggtgct cccgccccgc atcgcccctc
     3421 accaggtggt ggtgctcccc ctctggcgca aggacgagga gaaggcgggg gtcatggccg
     3481 ccgtggatag ggtggtcggc acgctcaagg gcgcgggggt gcgcgtgaag gtggacagcg
     3541 atgacagcaa gacggtcggc tggaagcaca acgagtggga gctgcgcggg gtgcccttgc
     3601 gcatggcgat aggccccagg gacgtggaga acggcacggt ggaactggcg aggcgcgata
     3661 agcccggcaa ggagttcaag gaatacccca gcatagactc gctggccgag tgcgtgcccc
     3721 aactgctcgc ggagatacag cagaacctct accagcgcgc cctggagttc cgcgtgcgca
     3781 acacccgcca cgtagccgat atggaggagc ttgcccaggc gatagaggag cggcagttcg
     3841 cggacgcctt ctggtgcggc atgagcgagt gtgagaacta catcaaggaa gcggtgaagg
     3901 ccaccaaccg ctgcatgccc ctcgaccagc ccggcgacag cggcccctgc gtggtgtgcg
     3961 cgatgggcag caaagtgcac gcgatattcg ccagggcata ttaggcgatt ttagattttg
     4021 gatttggctg gtaaagggct aggggcacag gtcgatacgg ccctgcgagt cctggtggga
     4081 ttgccattgg aggacgcggg ccgtgcctac agcctccaat ggtgttctgt tggggaagta
     4141 cgcgtcgtac cggcccatca agggggcacg agggaggtcg gtgagtatgc attgcacgtg
     4201 cagtgcgagt ggcgcatcac aggtccggag gggctaattg tgggcctgaa cgaccgctat
     4261 ttcccggcgg gagatgcttt tgacaagcca cccgatttca actgggacgt gcagggcgcg
     4321 aacaggctcg atgagcggct gagtcgcttc atggagcagc gaagaagccg gcccttagtc
     4381 gtggagcagg tctgggccga ccatgtgggt agctttcgct tgctgtttga cggcggctac
     4441 tccctagacg tgttcccttt tgagactacg aagtacgaat actggaggct cttcaaaccg
     4501 ggcctgcccg acgccggtga agtgccccac tttgtagtca ccggagaggg tctggaagat
     4561 tgagcgcccg gcaacctgcc actagggtaa acagccaggg ggaaaaggcg gcctatctca
     4621 gcctgctact catagccacc ctggtgctgt tcgccggact cctcccccgc ttcccctcca
     4681 cagccaccac cctccccaca cccaccccaa ctactcaaac caaaatccaa aatccaaaat
     4741 ccaaaatcgg cctccacacc cggctgaccg acgagtcgga cgctgagaaa atacggcagg
     4801 agttccgcat gctgcgcgag atgggcggca gctacgcggt cgagttcttc ccctgggcct
     4861 atatccaggc ccacgaccgc aaccgcttcg actgggagca cgccgacctc gtggtcgacg
     4921 ccgcgcggga gtacggcatc acgctcatcg cgcgcgtgga cggggtgccc aactgggcca
     4981 agccggagaa cgccacctgg cgctacctcg accgcgagca ctacgacgac tacgccgctt
     5041 tcgtggcggc cttcgtgcag cacttcaagg gccgcatagg gcactacatc atctggaacg
     5101 agccgaacac cgccgccgag tgggggcagc gcccgcccga cccggagggc tacgcggagc
     5161 tgctgaaggt agtgtacacc cgcgcaaagc aggcggaccc ctccgctgtc atcatgctgg
     5221 cggggctggc ccccaacacc gagcacgagg gcagccccaa cgccatgagc gacctggcgt
     5281 acctggaccg cctctaccgg gcaggcgcgg ccccctactt cgacgccgtg gccgtccact
     5341 cctacggcct caccgccccg cccggcgacc ccgcctcccc cggaaaggtc aactgggcga
     5401 ggacggagga gatataccgt gtgatggtgc agcacggcga cggcgacacg cccgtgtaca
     5461 tcaccgaggg cggctggaac gatagcccgc gctggtcctt cgcggtgaag ccgtaccaga
     5521 gggtagagta tacggtggag gcgtatcgca tggccgagga gcagtggccg tgggtgcgcg
     5581 ccgtgtgcct gtgggcctcc cgcttccccc gcccgcagta cacctatttc gacaactact
     5641 ccttcctcac gcccgatttc atccccaagc cggtgtacct cgaagtgcag caatacgccg
     5701 agcgtggggg taggtaatgt gatggtaccg cgaatgaata agtgtcctga acaggaccat
     5761 gttgacctga ggctttggca cacattagcc cacctcatcc aaacaccaac gcacattgag
     5821 acatatgcct cgtaacctcc tcaacatagc cgttgtcctc gccctcctgg ccgtctccgc
     5881 gctggtgtgg ctggggcagc agaagccgcc agaccgcttc ctggagcgca tccggcagac
     5941 cggcaccctg cgcgtgggca tggacccgac ctacccgccg ttcgacgtgg tgcaggatgg
     6001 ccaggtgagg ggctacgacg cggcgctggc gcaggccata gcgcgcgacc tcggcgtgca
     6061 ggtggagttc aagacgctcg cgctggatac gctatacgat gctctgatcg cgggcaacgt
     6121 ggacatgctc gtctcggccc tgcctccgat accggagcgg caagccgacg tgcgctattc
     6181 ggtgccgtat taccagtcgg ggcaggtgct ggtcgtcagg tcgggggaga aggccattat
     6241 cgggattggg gggctggcgg gcaagaaagt aggagtcgaa ctaggctcca acgccgatac
     6301 ggaggcgagg cggttgcagc gcagcacagc gccgggtatg cagctaagct cggtctacc
//
LOCUS       DASRWZ010000539         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_317734, whole genome shotgun sequence.
ACCESSION   DASRWZ010000539 DASRWZ010000000
VERSION     DASRWZ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317734"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..881
                     /locus_tag="VFH60_08440"
     CDS             39..881
                     /locus_tag="VFH60_08440"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET6313849.1"
                     /translation="MVNDLRERITLTTEAIRRHLPEGWSPQVGIILGSGLGALADEVK
                     AVASLPYSEVPGFAQSSVIGHVGRLVLGELEGTRVLVMQGRLHYYEGYSLQQVTFPVR
                     VMRALGAHTLVVTNAAGGLNPDFRAGDLMLIEDHINMLGWGGHNPLMGPNDPKLGPRF
                     PAMNPAYDLGLRKLAIEVAAECNITLRRGVYIVVAGPNYETGAELRLLRQWGADAVGM
                     STVPETLVAVHGGMKVVGISNITNMALAGSEEPVNHEEVLLVAEESRPRFITLMKAIV
                     ARIT"
     gene            941..1798
                     /locus_tag="VFH60_08445"
     CDS             941..1798
                     /locus_tag="VFH60_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313850.1"
                     /translation="MAGERSSGEELFGKGLGIAVAFGALAVGVAVAMANNERTRQMRD
                     ELRVRLDDLGRRVDDLSVQAQRMVEERRPEIEDTIQRSRQTIIEGLDKAKTIAEQGAD
                     KARSVSGEGAERAKSAAEKGAEMAKSVVEQGAEKAQEYVQRISHNGATEAADLADKAG
                     DAVGDVADDASRAMRDMDDEAQQAKDKMDREADEALSGIASDFDQAMAGMERDADEAM
                     DRINRMGDEALAMADEGNEDSEGDDTQPDTGKQKGRYNTGSLDDGDDTAGDGEPPHPG
                     DTRNDRSET"
     gene            complement(1847..>2975)
                     /locus_tag="VFH60_08450"
     CDS             complement(1847..>2975)
                     /locus_tag="VFH60_08450"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET6313851.1"
                     /translation="LLRTRNLPNLREWVRHPAVWLAVALAAIGVLALFVVPGGEARRV
                     ALREFRWTVVEPVLFFALMLRWLRTERDAWRMVGAWLIAAALVGREGVEQFLFGETWR
                     MEGVGRASSVYPSATAFGIYLGRPLALGIVLAFFLPGKWLTWRVGAALLSVVMGLGVL
                     FSFARGAWVGVFVALVVVAILTRHRWLISGIGAAIVAGIAALPFINVTRITSAFDFST
                     EENTGVARGKIWSAGMRIIADHPITGIGQDQFLRQDPSYGVPQTRFFLTSHPHNWILD
                     FWLRLGAPGLLWMVAALSYFFHQAWQLWKVYKGTALGALALGLIAAMIDFVVHGLMDM
                     AYFTMDNALSFWLMLGLVVMLKRGQRGQGSGIRDQGLGVRE"
BASE COUNT          662 a          904 c          962 g          447 t
ORIGIN      
        1 ctggttattc tgcttaaggg acgttagggg gctagcatat ggtaaacgat ttgcgggaga
       61 ggataacgct cacgacggaa gcaatacgtc gtcatctgcc cgaaggatgg tccccccagg
      121 tgggcatcat cctcggctca ggattgggtg ctctggcgga cgaggtgaag gctgtggctt
      181 cgctgcctta ctcggaggtg ccggggttcg cccaatcttc ggtcatcggg cacgtcggca
      241 ggctggtgct gggggagctg gagggcacac gagtgcttgt gatgcagggg cggctccact
      301 attatgaggg ctacagtctc cagcaggtga ctttccccgt gcgcgtcatg cgggcgttgg
      361 gcgcgcacac gctggtcgtg accaacgcgg cgggcggcct caaccccgat ttccgggcgg
      421 gcgacctgat gctcatagag gaccacatca acatgctggg gtggggcggg cacaacccgc
      481 tcatggggcc gaacgacccg aagctaggcc cgcgcttccc cgccatgaac ccggcctacg
      541 acctcggcct gcgaaagttg gctatagagg tggcggcgga gtgcaacata accctgcgga
      601 ggggcgtata catagtagtc gccgggccga actacgagac gggggccgag cttcgcctcc
      661 tgcgccagtg gggcgcggac gcggtcggca tgtctaccgt gcctgaaacg ctcgtagcgg
      721 tgcatggtgg catgaaggtt gtaggtatat ccaacatcac caacatggcc ctcgccggca
      781 gcgaagagcc tgtcaaccac gaagaagtgc tgctggtagc cgaagaatct cgcccccgct
      841 tcataacgtt gatgaaagcg atagtggcga gaataacgta aaacgtaagg aactcagcac
      901 tcagcactca gcactcggta ctcaaataag gaggtagcaa atggctggag agagatcgtc
      961 gggtgaagaa ctctttggga agggattagg catcgcggtg gcctttggcg cgctcgccgt
     1021 cggggtagcc gtagcaatgg cgaataacga gaggacgcgg cagatgcgcg atgaattgcg
     1081 tgtgaggctg gacgacctgg ggcggcgcgt ggacgaccta tcggtccagg cgcaacgcat
     1141 ggtcgaggag cgcaggccgg agatagagga caccatccag cgcagccgcc agactataat
     1201 cgaaggtctg gacaaggcga agaccatagc cgagcagggc gcggacaaag caaggtccgt
     1261 gtcaggtgag ggcgcggagc gtgccaaatc ggcagccgag aagggcgctg agatggcgaa
     1321 gtcggtagtg gagcagggcg cggagaaggc gcaggaatac gtacagcgca tcagccacaa
     1381 cggcgctacc gaggcagccg atttagccga caaagccggg gatgcggtag gcgacgtggc
     1441 cgacgacgcg agccgggcga tgcgcgacat ggacgatgag gctcaacagg cgaaggataa
     1501 aatggaccgc gaagccgatg aggccctaag cggcattgcc tccgatttcg accaggcaat
     1561 ggccgggatg gagcgcgacg ccgacgaggc tatggaccgc atcaacagaa tgggtgacga
     1621 ggcgcttgcg atggcagacg agggtaatga ggacagcgaa ggtgacgaca cccagcccga
     1681 cacaggcaag cagaaaggcc gctacaacac cggttccctg gacgacgggg atgacacggc
     1741 aggagatggc gagcctcccc acccaggcga cacgcgcaac gaccgttcgg agacgtaggg
     1801 atcaggggtt aggtatcagg gatcagggat caggggtcag catcgctcat tctctaaccc
     1861 ctaacccctg atccctgatc cctgatccct gacccctctg tccccgcttg agcataacca
     1921 cgaggccgag catgagccag aaactgagcg cgttgtccat tgtgaagtag gccatatcca
     1981 tcaggccgtg tactacgaaa tctatcatcg ccgcgatcag gccgagcgcc aacgcgccca
     2041 gggccgtgcc tttgtaaacc ttccaaagct gccatgcctg gtggaagaaa taggacaggg
     2101 ctgccaccat ccagagcagg ccgggagcgc ctagcctgag ccagaagtcg agtatccagt
     2161 tgtgcgggtg cgaggtcagg aagaagcggg tctggggcac accatagctg gggtcctgcc
     2221 gcaaaaattg gtcctgcccg atccctgtga tggggtggtc ggctatgatg cgcatgcccg
     2281 cgctccatat cttcccgcga gccacgcccg tattctcctc agtcgagaag tcgaacgccg
     2341 aggtgatgcg cgtcacgttg atgaagggga gcgcggctat acccgccaca atcgccgcgc
     2401 caatgcccga tatgagccac ctgtggcggg tgagtatcgc gaccaccacc agcgccacga
     2461 acacccctac ccacgcgccg cgagcgaagc tgaacagcac ccccaggccc atcaccactg
     2521 agagaagcgc ggcccccacc cgccacgtta gccacttgcc cggcaggaaa aaggcgagca
     2581 cgatccccaa cgcgagcggc ctgcccaggt agatgccgaa ggccgtcgcg cttgggtaca
     2641 ccgacgaggc gcggcccacc ccctccatgc gccaggtctc cccaaacaga aactgctcta
     2701 ccccctcccg ccccaccagc gcagccgcta tcaaccacgc ccccaccatg cgccatgcgt
     2761 cgcgctcggt acgcagccag cgcagcatca gtgcgaagaa aagcaccggc tccacgacag
     2821 tccaccggaa ctcgcgcaga gcgacccttc tagcctcccc gcccggtacg acaaatagcg
     2881 ccagcacgcc tatcgcagcc agcgcgacag ccagccagac cgcagggtgc cgcacccact
     2941 ctctcagatt gggcaggttg cgggtgcgca acaac
//
LOCUS       DASRWZ010000540         3817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_592390, whole genome shotgun sequence.
ACCESSION   DASRWZ010000540 DASRWZ010000000
VERSION     DASRWZ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592390"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1515
                     /locus_tag="VFH60_08455"
     CDS             112..1515
                     /locus_tag="VFH60_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6313852.1"
                     /translation="MPRKPKQAAQAEPQPGNLAPEAQQLVEEFRGRYPFPLDPFQEEA
                     IAHRAAGESVMVAAPTGTGKTVVAEFGIFRAARQGFKVMYTTPIKALSNQKFRDLREQ
                     YGDAVGLLTGDVIENREGSIIVMTTEVLRNMLLQTPSELWGVGCIIFDEVHYLADPER
                     GTTWEESIIMCPKYVQLVCLSATVTNAPEIADWITRTHQPVHLVTHLQRAVPLSLYYF
                     LDGKLNMVINARGQQVVDFNVGGEQKRKMQGGRYRGPTLEEDTNTQRERREPGPREIV
                     EELERADLLPAIYFQFSRRDCEAAAEICSMTRLKAMSDRRTLQRINEVLASYIERLTP
                     EDRKLEQVQFITYLARRGFGFHHAGLLPILKQLVEELFNKSLMRVVFATDTLALGVNM
                     PARTVVIGGMSKYDGVSRRPLIPNEFQQMSGRAGRRGIDEQGHVVLPYSPWVTFHEAL
                     NIATGPLRQEESAFTIR"
     gene            1522..3096
                     /locus_tag="VFH60_08460"
     CDS             1522..3096
                     /locus_tag="VFH60_08460"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313853.1"
                     /translation="MLNLWDAPAGERVLTVMRASLLQFQQGRRLRDLEQELEEQESRL
                     AEVPAGCLIGYDNGEELLDEYEHRGREVEDLRRREKKADQNVRYIESRRDELPWRRPE
                     RDQLRKAFRNLPQGALVHLEQQGWAAYLGRGLGGAVIGLFLPVSQGEEGVQLGEIFPL
                     IEYRQIDYLHTVTVTVDLPYALTTLAVPPTLPLDELLPAEELEQLRHSAALGLQAVPD
                     IEAWKTEYRAAQVEHIEEASAKAKANLEEVRAELTVAARAEREHVCHDCPVRKEHRSN
                     RRLRARLLLDRDAAEKRLSERRHYEETRLQTTLKGLTSVLVQFAYLRKGELTYKARKL
                     ADVFDTNALMIMEMVEGGYLDGLKPQDLAEVFSWFAYDRDIDFLNRLLLPRYLLNLRR
                     DLDSLQNAIFAAERRQDLTLTRGYNPYFYGAARAWCKGTSIADLLDQMQMSEGDLVMT
                     FNKTLDLMRQVRDMLAHHDPQNPLRATFSEADKLMRRGVVEMAGMIGFAPRTESEAAD
                     AEPADTDGDDPADLDK"
     gene            3244..3723
                     /locus_tag="VFH60_08465"
     CDS             3244..3723
                     /locus_tag="VFH60_08465"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET6313854.1"
                     /translation="MSDSTSAAKPLEVFYAYAREDEKLRKKLNKSISILIADGVIAPW
                     YDRDVSAGLEWEHVIDEHLNRANIILLLISLDFIGSPYCYGIEMKRAMERHEAGEARV
                     IPILLRAIDWEKAPFRKLAGLPSNGKPVTSWSNRDEAYADIAKGIRKVAEELRPNPR"
BASE COUNT          789 a         1312 c         1107 g          609 t
ORIGIN      
        1 tttgccattt cagtgaaccc ccttagtcag atataagcgc ccttccttac cctgccgtac
       61 tcacggccca cctcttgctc atatctccat gacacttata tatcaatcct catgcctaga
      121 aaacccaagc aggccgccca ggccgaacca caacctggca acctcgcccc cgaagcccaa
      181 cagctcgtag aggagtttcg cgggcgctac cccttccccc tcgacccgtt ccaggaggag
      241 gctatcgcgc accgtgcggc gggcgagagc gtcatggtgg ccgcacctac cggcaccggc
      301 aagaccgtcg tggccgagtt cggcatcttc cgcgccgcac gccagggctt caaggtgatg
      361 tacaccaccc ccatcaaagc cctctccaac cagaagttcc gcgacctgcg cgagcagtac
      421 ggcgacgccg tgggcctcct cacaggcgac gtgatagaga accgcgaagg ctctatcatc
      481 gtcatgacca ccgaggtgct gcgcaacatg ctcctccaga ccccctccga gctttggggc
      541 gtcggctgca tcatcttcga cgaggtccac tacctggccg acccggagcg cggcaccacc
      601 tgggaagagt cgatcatcat gtgccccaag tacgtgcaac tggtctgcct ctccgccacg
      661 gtcaccaacg cccccgaaat cgcggactgg atcacccgca cccaccagcc cgtccacctg
      721 gtcacccacc tgcaacgcgc cgtgcccctc tccctctact acttcctcga cggcaagctc
      781 aacatggtca tcaacgcccg gggccagcag gtggtggact tcaacgtggg cggcgagcaa
      841 aagcgcaaga tgcagggcgg ccgctaccgt ggccccacgc tggaagaaga caccaacacc
      901 cagcgagagc gccgcgaacc cggcccccgc gagatcgtgg aggagcttga gcgggccgac
      961 ctgctccccg ccatctactt ccagttctcc cgccgcgact gcgaggccgc cgccgaaatc
     1021 tgctccatga cccgcctcaa ggcgatgagc gaccgccgca ccctgcaacg catcaacgag
     1081 gtgctggcga gctacataga gcgcctcacc cccgaagacc gcaagttgga gcaggtgcag
     1141 ttcatcacct acctagcgcg gcgtggcttc ggcttccacc acgcgggcct cctccccatc
     1201 ctcaagcagc ttgtggagga gcttttcaac aagtccctca tgcgcgtggt gttcgccacc
     1261 gacacgttag ccctcggcgt gaacatgccc gcccgtacgg tggtcatcgg cggcatgagc
     1321 aagtacgacg gcgtgtcccg ccgccccctc atccccaacg agttccagca gatgtcgggt
     1381 cgcgcgggcc gccggggcat cgacgagcag ggccacgtgg tgctgcctta ctccccgtgg
     1441 gtcactttcc acgaggcgct gaacatcgcc acggggccgc tgcgccagga ggagagcgcc
     1501 ttcacgatac gctaaaactc ggtgctcaac ctctgggacg caccggcggg cgagcgcgtg
     1561 ctcaccgtca tgcgggcctc cctcctgcaa ttccagcagg gccgccgctt gcgcgacctg
     1621 gaacaggagc ttgaagagca ggagtcccgc ctcgcggagg tgcccgccgg gtgcctcatc
     1681 ggctacgaca acggcgagga actgctggac gagtacgagc accggggccg cgaggtggaa
     1741 gacctgcgcc gccgcgagaa aaaggccgac cagaacgtgc gctacatcga gtcgcggcgt
     1801 gacgagctgc cctggcgacg ccccgagcgc gaccaactcc gcaaagcctt ccgcaacctg
     1861 ccgcagggtg cgctggtgca cctggagcag cagggctggg ccgcctacct gggccggggc
     1921 ctgggtgggg ccgtgattgg gctgttcctc cccgtgagcc agggcgaaga gggcgtacag
     1981 cttggcgaga tattccccct catcgagtac cggcagatag actacctgca caccgtcact
     2041 gtcaccgtag acctacccta cgccctgacc accctggccg tgccgccgac cctgcccctt
     2101 gatgagttgc tgcctgccga ggagttggag cagctacgtc acagcgccgc cctggggcta
     2161 caggcagtgc ccgacattga ggcgtggaaa actgagtacc gcgcggcgca ggtggagcac
     2221 atcgaggaag cgtccgcgaa ggcaaaagcc aacctggagg aagtgcgggc cgagcttacc
     2281 gtcgctgcca gggccgagag ggagcacgtc tgccacgact gcccggtccg caaggagcac
     2341 aggagcaacc gccgcctgcg cgcccgcctg ctgctcgacc gcgacgccgc cgagaagcgc
     2401 ctctccgagc gccgccacta cgaggagacc cgcctgcaaa ccacgctcaa gggccttaca
     2461 tccgtgctgg tgcagttcgc atacctgcgc aagggcgagc taacctacaa ggcccgcaag
     2521 ctggctgacg tcttcgacac caacgccctc atgatcatgg agatggtgga gggtggctac
     2581 ctcgatgggc tgaagccgca ggacctggcc gaagtcttct cctggttcgc ctacgaccgc
     2641 gacatcgact tcctcaaccg cctgctcctg ccgcgctacc tgctcaacct gcgccgcgac
     2701 ctcgacagcc tgcaaaacgc catcttcgcc gccgagcgcc gccaggacct caccctcacg
     2761 cggggctaca acccctattt ctacggggcc gcccgcgcct ggtgcaaagg cacctccatt
     2821 gccgacctgc tcgaccagat gcagatgagc gagggcgacc tcgtgatgac cttcaacaag
     2881 acgctggacc tcatgcgcca ggtccgagac atgctggccc accacgaccc acagaacccc
     2941 ctccgcgcca ccttctccga agccgacaag ctgatgcgcc gtggcgtggt ggagatggcg
     3001 gggatgatag gcttcgcgcc aaggaccgag tctgaagccg ccgacgcgga acccgccgat
     3061 acagacggcg acgatccggc ggacctggat aagtagcatt tagcctaccc ttccacgttc
     3121 ccctgcctcc gtccatcgcc ccgcaagcat cgggctagca cttccctaca aggattagta
     3181 cagctataat gtggctgtac gcgaaccgag gtgttctgca acatcgctct aggggagagg
     3241 cgcatgtctg attcaacaag tgcagcaaag ccgcttgagg tgttctatgc ttacgcgcgg
     3301 gaagatgaaa agctgcggaa gaagctaaac aagagcatca gcatattgat agctgacggt
     3361 gtgattgccc catggtacga ccgagacgta agtgctggtc tggaatggga gcacgtgata
     3421 gatgaacacc tgaatagggc taatattatc ctgctcctca tcagtctaga cttcatcggc
     3481 tctccatact gctacggcat cgagatgaag cgagctatgg agcggcacga ggcaggggaa
     3541 gccagggtaa ttccgatcct cctcagggcg atagattggg agaaggcacc tttcaggaag
     3601 ctagcaggtc tgccctcgaa tggaaagcct gtcacaagct ggtcaaacag agatgaggcg
     3661 tatgcagaca tagcaaaggg cataaggaaa gtagccgagg agttacgccc aaaccccagg
     3721 taggctcaac gtcttcatcc agcaccacga cgttgagcca gaacaaggtg ccagtactac
     3781 ccagggtctg gaccgtgcca cacccgcgca atcccaa
//
LOCUS       DASRWZ010000541         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1071806, whole genome shotgun sequence.
ACCESSION   DASRWZ010000541 DASRWZ010000000
VERSION     DASRWZ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071806"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFH60_08470"
     CDS             complement(<1..541)
                     /locus_tag="VFH60_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313855.1"
                     /translation="MSTEVATREAVAAREAKAREGNLTMMRRIKLAFKLVLVLLATVF
                     ALYPVAWIISASFNPLNSLVGLSFIPNGATFANYQRLYTDPQTPFMLWIFNSAKIGVI
                     CAVIIVSLTALSAFAFSRFNFPGRRGTLLTVMLVQLFPNTLALVALFLMLQQIGNIRG
                     LEWLGLNTHGGLILIYIGGA"
     gene            complement(538..2157)
                     /gene="malF"
                     /locus_tag="VFH60_08475"
     CDS             complement(538..2157)
                     /gene="malF"
                     /locus_tag="VFH60_08475"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008232.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HET6313856.1"
                     /translation="MQPTPSAASSPRPSAGDSGSDNKGGARGRASLPTSNLLLRILGL
                     IFIDAVAFYFAYALFGQGQGAVAVILLVVTGIVNWLFIDDRVYPWRWFTPGLLLMLLM
                     VVYPLGFTLYTSLTNYSDGHLLTKEQVLNQFTSQYFQPENAVSYTWTAYRNPDGTFLL
                     VFQDPQGNRLVGNEQEGLKPLPGAGDLPASLTDSTGKPYQKLELAQTLQYLGTLTKMQ
                     MKSGDKLVRVTSLGRGSESLAQYVYDPATDRLTDQQTGVVYTPVRGSFTAPDGKTLSP
                     GFSVVVGFENYTRVLTDDNIKGPFVSVFIWNLAFALLSVLFTFVVGLGMALILNDKDL
                     PFKGVWRSLIVIPYAIPAVISTVVWVGLLNPNYGQFSGLIANIFGDSPEWFSNGFWAK
                     VGILLINTWLGYPYMMLICLGALQSIPTDMYEAANIDGAPAWTQFSRLTLPLLLVAIA
                     PLLIGSFAFNFNNFGAIEAYNRGGPPIPGASTPAGQTDILLSYTFRLAFGSGQGADYG
                     LASAIGLFIFVLVAGLTAFNFRFTRRLENLT"
     gene            complement(2117..3532)
                     /locus_tag="VFH60_08480"
     CDS             complement(2117..3532)
                     /locus_tag="VFH60_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6313857.1"
                     /translation="MRKLIKLVVLLMLTSMVLAACGGPAATPVPATATNAPAADTATM
                     PVAEDTATTEAQEPAVEDTATMAPAADDTATTAPEAAATPTVAIGRPSEEGVLTLWVQ
                     RLAVPAVTEAGKQFTAKYNIPVSVQELEFGSIRDQLKTAGPAGEGPDIIAGAHDWLGE
                     LVSNGLIEPLDLGEKAASFDPVAVQAFTYEGKVYGLPYALEAIALLYNKDLVPTPPAT
                     WDELKTIAKQLQDEGKVDQGFVLQQGDPYHSFPLWTGFGGYVFKQNPDGTYDPTDVGL
                     DNPGGVKAMTEIDQMIKEGLLRPDITGQIGETLFATGKSAMTVGGPWRLADLRKAGIN
                     YGIAPLPKMDVQPQPFVGSQGFMVSAFGKNKEIAQAFLTEFMAGDEPMQAMYTADPRI
                     PAWLPVQQKVASSTDPNDVDISGFAKSATVGMPMPAIPQMSSVWEDWTKAINLVYQQQ
                     EEPGKAITDAANSIRSKLTTP"
BASE COUNT          663 a         1023 c         1227 g          661 t
ORIGIN      
        1 gcgcgccgcc gatgtagatg aggatcaggc cgccgtgggt gttgaggccc agccactcaa
       61 gcccccggat gttgccgatc tgctggagca tcaggaagag ggccaccagc gccagcgtgt
      121 tggggaagag ctgcacgagc atgaccgtga ggagcgtgcc gcgcctgccg gggaagttga
      181 agcggctgaa ggcgaacgcg gacagcgcgg tcagcgacac gatgattacg gcgcaaatca
      241 ccccgatctt cgcggagttg aatatccaca gcatgaacgg ggtctgcggg tcggtgtaga
      301 ggcgctgata gttggcgaag gtggccccgt tgggtatgaa actcaggccc acgagcgagt
      361 tgagcgggtt gaacgaggcc gatatgatcc aggccaccgg gtagagcgcg aagacggttg
      421 ccagcagcac cagcacgagc ttgaacgcca gcttgatgcg ccgcatcatt gtaaggttgc
      481 cttcgcgggc cttggcctcc cttgcggcga ctgcctcgcg agttgcgact tctgtgctca
      541 tgtcaggttc tccagtcgcc ttgtgaagcg gaagttgaag gcggtaagcc cggccaccaa
      601 cacgaagatg aagaggccga tggccgaggc aaggccgtag tcagcgccct gccctgagcc
      661 gaaagccagg cggaaggtat aggagagcag gatatcggtc tgccccgccg gggtcgaggc
      721 ccctgggatg ggcggacctc ctctgttata ggcttcaatc gcgccgaagt tgttgaagtt
      781 gaaggcgaac gagccaatca gcaggggcgc gatggccacc aggagcaacg gcagggtaag
      841 cctgctgaac tgcgtccagg ccggtgcgcc gtcgatgttg gcggcttcgt acatatcggt
      901 cgggatagat tggagcgcgc ccaggcagat gagcatcatg tacgggtagc cgagccaggt
      961 gttgatgagc aggatgccca ctttggccca gaagccgttg gagaaccact ccggagagtc
     1021 gccgaaaatg ttggcgatca ggccgctgaa ctgcccgtag ttggggttga gcaggcccac
     1081 ccacaccacg gtgctgatga ccgccggtat ggcgtatgga atgacgatca gcgagcgcca
     1141 cacgcccttg aagggaaggt ccttatcgtt gaggatgagc gccatcccca ggcccaccac
     1201 gaaggtgaac agcaccgaca gcaaagcgaa cgccaggttc caaatgaata ccgagacgaa
     1261 cggccccttg atgttgtcgt cggtgagcac ccgcgtgtag ttctcgaagc ccaccaccac
     1321 gctgaagccc gggctgaggg tcttaccgtc gggggccgta aagctgcccc tcacaggggt
     1381 gtagaccacg ccggtctgct ggtcggtgag cctgtcggtc gccgggtcgt agacgtactg
     1441 ggccagcgac tcgctgcctc gccccaggct ggtgacgcgc accagtttgt cgccgctctt
     1501 catctgcatt ttcgtgagcg tgcccaggta ctggagggtc tgcgcaagct ctagcttctg
     1561 gtacggcttg cctgtggagt cggtaaggct ggcgggcagg tctcccgcgc cggggagcgg
     1621 cttgaggccc tcctgctcgt tgccgaccag gcgattaccc tgcgggtcct ggaaaacgag
     1681 gaggaaagtg ccgtcggggt tgcgatacgc ggtccaggtg tagctgacgg cgttttcggg
     1741 ctggaaatac tggctggtga actggttcag cacctgttct ttggtcagga gatggccgtc
     1801 gctgtagttg gtaagcgagg tgtacagggt gaagccaagc gggtacacca ccatgaggag
     1861 catgagcagc aagcccggcg taaaccaccg ccaggggtag acccggtcgt cgatgaagag
     1921 ccagttgacg atgcccgtca ccacgagcag gatgacggcg accgcgccct ggccctggcc
     1981 gaagagggcg taggcgaagt aaaaagctac cgcgtcaatg aagatcaggc cgagtatacg
     2041 caggagcagg ttggaggttg ggagagaggc gcgcccgcgc gcgcctccct tgttatccga
     2101 acctgaatct ccagcgctag ggcgtggtga gcttgctgcg gatggagttg gctgcatcgg
     2161 tgatggcctt tccaggttct tcctgctgct ggtataccag gttgatagcc ttggtccagt
     2221 cctcccagac ggacgacatc tgcgggatgg cgggcatcgg catgccgacg gtggccgact
     2281 tggcgaagcc ggagatgtcc acgtcgttgg ggtcagtaga ggaagctacc ttttgctgaa
     2341 cgggcaacca ggccgggatg cgagggtcgg ccgtgtacat ggcctgcatc ggctcgtcgc
     2401 cggccatgaa ctcggtcagg aaggcctggg caatttcctt gttcttgccg aaggcggaga
     2461 ccatgaagcc ctgcgagcct acgaagggct gcggctgcac gtccatcttc ggaagtgggg
     2521 cgatgccata gttgataccg gccttgcgca ggtcggccag gcgccacggg ccgcccaccg
     2581 tcattgcgga cttgccggta gcgaacaacg tctcaccgat ctggccggtg atgtccgggc
     2641 gcaacaggcc ctccttgatc atctggtcga tctcggtcat ggccttcacg ccacccggat
     2701 tgtcgaggcc cacgtcggta ggatcgtagg tgccgtcggg gttctgcttg aagacgtagc
     2761 cgccgaagcc ggtccacagg gggaaggagt ggtaggggtc gccctgctgg aggacgaagc
     2821 cctggtcaac cttgccctcg tcctgcaact gcttggcgat agtcttcagc tcgtcccagg
     2881 tggcgggagg cgtgggcacc aggtccttgt tgtacaggag ggcgatagct tcgagagcat
     2941 acgggaggcc gtaaactttg ccctcatagg tgaacgcctg caccgccacg gggtcgaagc
     3001 tcgcggcctt ctcgcccagg tcgagcggct caatcaggcc gttggatacc agctcgccca
     3061 gccagtcgtg cgcgcccgcg atgatgtcgg ggccttcacc ggcggggccg gcggtcttta
     3121 gctggtcgcg gatagagccg aattcaagct cctgcacgga caccgggatg ttgtacttgg
     3181 cggtaaactg cttacccgct tcggtcacgg ccgggactgc caacctctgc acccacaggg
     3241 tcagcacgcc ttcctcggaa ggcctgccga tagctacggt aggcgtagca gcggcttcag
     3301 gcgccgtggt ggcggtgtcg tcggcggccg gtgccatagt ggcagtatcc tctaccgcag
     3361 gctcctgggc ctcggtggta gcggtatcct ccgccacggg catggtggcc gtatcggcgg
     3421 ccggcgcgtt ggttgccgtg gcgggcacgg gggtggccgc aggcccgcca cacgctgcga
     3481 gcaccatgct ggtgagcata agcaatacga ctaatttgat taacttccgc attgaagaac
     3541 tcctccttgt tatcttgact gtatgtccgg ggag
//
LOCUS       DASRWZ010000542         5474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1140696, whole genome shotgun sequence.
ACCESSION   DASRWZ010000542 DASRWZ010000000
VERSION     DASRWZ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140696"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1396
                     /locus_tag="VFH60_08485"
     CDS             260..1396
                     /locus_tag="VFH60_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313858.1"
                     /translation="MRNAPTNSHYFHSKVNQPMMSSERRTATNPSARQGRATRLWSLV
                     CLLGLGLAVVAPPAALAHEKWFVDATNYPVRWELVFSWPVAIALVAAGLALGGLVFLS
                     RVVRDPYWPNPVWLQPVNSSVQAVVGIQTAISLVYMTVRGWLLAPTLSVTGQWWGYVL
                     LVVQLFVAFTFVTGWLTRVGGGVLVGLVVAAFALFPPDRAAEQLLFAGIGAFFLIQGR
                     GLFAPAGQLSRRWAEYWSRYSRMALPVMRVFTGVSILWLAFSEKLLNPQLALAFLESR
                     PEFNFVQLLGFSWFTNELFVYAAAAVEATVGVLLIAGVLPRIVILFMWVPFNIAIPFL
                     PPEELLGHLPILAVMYAVFLGEPAVEAEARKAISPTPAPATSGG"
     gene            1850..3193
                     /locus_tag="VFH60_08490"
     CDS             1850..3193
                     /locus_tag="VFH60_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6313859.1"
                     /translation="MEIEAGSAGSNREPVSGIPSVGAQAPAPPTPTEQAEQAEPGKFK
                     LGLAVNYSVANLGASVVYGLFNFALPPYLATYNLHPSLIGLLANERSFVGAFVQPVIG
                     RISDRTTSPLGRRRPFFLIGIPLMAVSLLLLATHPPFWIMFAVMTISAFFLAIAWDPY
                     MAMMADLFPPAHRGRVGGLIGVGTALGNIIFALMAFFLIVNGEFLVFAVSTAIMVVCW
                     AYTFFTVKEPAVPAEPNAGQKMGKITPRQYIRSLREYPEAAKYTLAILFFWMGSGGAV
                     PFITLFGANVLNASDSEVFLLPLAATLSMAVFAVPWGIFADRTSKKTAMTLGLIMFSV
                     VAVVGSQSGTLLQGTIALALIGVGNSAMSLINPMLVDLVPRKRTAEFVGLGSAVFSFA
                     QPLGSAVAGAIVAGAEQFVGREDAYRWAFIFAGLMTALAALLLRAVRPEQFVDKD"
     gene            3225..3962
                     /locus_tag="VFH60_08495"
     CDS             3225..3962
                     /locus_tag="VFH60_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6313860.1"
                     /translation="MSVQSPVAEVIAPGQSEAVPQPRRRRVARAGLRLRLFRWVLRAV
                     FRLVFRVRVSGLEHVPDTPVIVCSNHLGWTDPFLVLLFLPIEPCIYVLGEKQVRRISW
                     LRRLVIDALGIMVALDRDKPLHALRTMSGVLHKGGSLLIFPEGHLGTEEGRLLELQDG
                     AAHLSQKSGVPLLPVGLTGTSKLWLRRTLTVCIGPPIQPHDFTGDLRTRLHSMTASLD
                     AAMRSLLPGDTEHPRLRLLERWLTRLL"
     gene            4040..4540
                     /locus_tag="VFH60_08500"
     CDS             4040..4540
                     /locus_tag="VFH60_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET6313861.1"
                     /translation="MGRPARIEPVGPGEESVWEYPRPPRLEDTAKHIQVVFNGVTVAD
                     TRRARRVLETSHPPVYYIPPEDVLQSALVPTTRSTYCEWKWHASYFSVRVGDKTAPNA
                     VWSYPDPSPGFEGIAGYLAFYPGPMDACLVDGEQVQSQPGSFYAGWVTSEIKGPFKGE
                     PGSSGW"
     gene            complement(4565..5290)
                     /locus_tag="VFH60_08505"
     CDS             complement(4565..5290)
                     /locus_tag="VFH60_08505"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET6313862.1"
                     /translation="MSAITPVTREYLEAIYNITVEGDPVVGVRLAEKFGVSPPNVAQV
                     LTRMERDGLIKMPGRGRGKAGEGVELTPEGRSQAELMLRQHRLAERFLVDVMGMDWVQ
                     AHEEAHNLERGMSAALEDQMVKLLGNPRTCPHGNPIPGQNSNTLDYLREQKAIRLSQA
                     EPGKTMKVLLISEVVEDETALLTYLGSLHVMPGALVTVLDRDPDDGSFHVSVVPRDAA
                     GGTKANTVSLTKELAAKIWLRDA"
BASE COUNT          862 a         1687 c         1822 g         1103 t
ORIGIN      
        1 gaagtaccag agttcctctg accatcctca tcctaagcgt tgtagactct cgcctaccca
       61 cctgatcctt cgctacgctc aggatgacag gggggagatg gtccgcctat cacagcagtg
      121 gcaagcgccg ggcttcggtc ctgcgcctcg tctttggggt ggttgcgcag cagtactact
      181 gggtggcaca atgagagttc aaggcgaaag gggaggcgtg gctctgttct tgatacgccg
      241 aaggaggggg ttgcggcctt tgcgtaacgc acccaccaac agccactact tccactcgaa
      301 agtgaaccag ccaatgatgt ccagcgaacg aagaaccgcc acaaatccca gcgcgcggca
      361 gggacgcgct actcgactct ggtccctggt atgcttgctt gggcttgggc tggcagttgt
      421 cgcgccgccc gcggccctgg cccatgagaa atggtttgtg gatgccacga actaccccgt
      481 gcggtgggag ttggtcttta gctggcccgt agccatagcg cttgtggcgg cggggttggc
      541 cctgggcggg cttgtgttcc tcagccgggt ggtgcgcgat ccctactggc ccaatcccgt
      601 gtggttgcag cccgtcaact cgtcggtgca ggcggtggtc ggcatacaga cggccatcag
      661 cctggtgtac atgacggtgc gcggctggtt gctggccccc accttgagcg tcacggggca
      721 gtggtggggt tacgtgttgc tggtggtgca gttgttcgtg gccttcacct tcgtcacggg
      781 ctggcttacg cgggtgggcg ggggcgtact ggttgggctg gtggtggcgg cgtttgccct
      841 attccccccg gacagggccg ccgagcagtt gctgttcgcg ggcatagggg cattcttcct
      901 catccagggg cgggggctgt tcgccccggc agggcagctc tcgcgcaggt gggccgagta
      961 ctggtcccgc tattcccgca tggctctgcc ggtgatgcgc gtgttcacgg gcgtctcgat
     1021 cctgtggctg gcgttcagcg agaagctgtt gaacccccaa ctggcgctgg ccttccttga
     1081 gagccgcccc gagttcaatt tcgtgcaact gctcggcttt agctggttca ccaacgaact
     1141 gttcgtgtac gcggccgccg ccgtggaggc cacggtgggc gtgctgctga tagccggggt
     1201 gctgcctcgc atcgtgatct tgttcatgtg ggtgccgttc aacatagcga ttccgttctt
     1261 gccgccggag gagctgctgg gccacctgcc tatcctggcg gtgatgtacg ccgtgttcct
     1321 gggcgaaccg gctgtcgaag cggaagcccg caaagcgata agccccactc ccgcgcccgc
     1381 aacctccggg ggctgagggc tgtaggggca cactgcgccg tgctctgctg ctttccccgg
     1441 tgtggagcgg ttagaaccaa gccccacctt gtcagcctgt attccccttc tcatcctgcg
     1501 cgcagcgaag agattgccct gagcggagcc gaagcgttcg aaatgacagg ggaggggctt
     1561 ggtgcgatgc gcccatacgg ttgtgctgct ggtctaggca taattgccta tagggagcca
     1621 ccgtctcgat gtgacaacgt agtaattctg ccgaagccgg gccgttgttc tccggctatg
     1681 atgtcatcgt gaaaccctgt tgccaggacc ggtactctta gcagaggtgc ggggccgagt
     1741 cgtgagaagc ggccgtacta tttacttttt ggttagcacg gcagtataat ccgcacacaa
     1801 ggccgcttcc caagtgggtc tgcggactag cccaaacgag gtgatcgtga tggagataga
     1861 ggcaggatcg gccggttcta acagggagcc ggtgtcgggc attcccagtg ttggagcgca
     1921 agcgcccgct ccacccaccc cgactgagca ggctgagcag gctgagccgg gcaagttcaa
     1981 gctgggactg gcggtgaact acagcgtcgc caacctgggc gcatcggtgg tctacggcct
     2041 gttcaacttc gcgttgcccc cataccttgc cacctacaac ctgcacccgt cgctcatcgg
     2101 cctgctggcg aacgagcgct cctttgtggg ggccttcgta cagcctgtga ttggcaggat
     2161 tagcgaccgc accaccagcc ctctggggcg caggcggcct ttcttcctca tcggcatccc
     2221 gctcatggcc gtgtccctct tgctgctggc tacccaccct cccttctgga tcatgttcgc
     2281 ggtaatgacc atatccgcgt tcttcctggc gattgcctgg gacccctaca tggcaatgat
     2341 ggccgacctc tttccccccg cccacagggg cagggtaggg ggcctcatag gcgtgggcac
     2401 ggcgctgggt aacatcatct tcgcgctcat ggcattcttc ctgatcgtca acggcgagtt
     2461 cctggtgttc gccgtgagca cggctatcat ggtcgtgtgt tgggcctata ccttcttcac
     2521 ggtgaaagag cctgcggtgc ccgccgagcc gaacgcgggc cagaagatgg gtaaaattac
     2581 ccccagacag tacatcagga gcctgcgcga ataccccgaa gcggccaagt acacccttgc
     2641 catactcttc ttctggatgg gttctggcgg tgcggtgccc ttcataacgc tgttcggggc
     2701 taacgtgctg aacgcaagcg acagcgaggt gttcctccta ccgctggcgg cgaccctttc
     2761 gatggccgtg ttcgcggtgc cctggggcat cttcgcggac cgcaccagca agaagaccgc
     2821 catgaccctg ggcctcatca tgttttcggt ggtagccgta gtcggctcgc agagcggcac
     2881 gctgttgcag ggcactatcg cgctggcgct catcggggtg ggcaactcgg ccatgtcgct
     2941 cattaacccc atgctggtag acctggtgcc ccgcaagcgc accgccgagt tcgtcggcct
     3001 cggctcggcc gtattctcct tcgcgcaacc gctggggtcg gctgttgccg gcgcgattgt
     3061 ggcgggggcg gaacagttcg tcgggcggga ggacgcctac cgttgggcct tcatcttcgc
     3121 cgggctgatg acggccctgg cagctctcct actgcgcgcg gtgcgccccg aacagttcgt
     3181 ggataaagac tgagcgccac ggctcagagg gtaacgtgct tttcatgagc gtgcagtcac
     3241 cagtcgcgga agttatcgcg ccaggccaga gcgaggccgt tccccagcct cggcgtaggc
     3301 gtgtggcgag ggcgggccta cggttgcgcc tgttcaggtg ggtgttgcgc gccgtgttca
     3361 ggctggtgtt cagggtgcgg gtgagcggct tggagcacgt acctgacacg ccggtgatcg
     3421 tttgctcgaa ccacctgggc tggaccgacc ctttcctggt gctgttgttc ctgcccatcg
     3481 agccgtgcat ctacgtgctg ggcgaaaagc aggtgcggcg tatctcctgg ctgcggaggc
     3541 tcgtcataga cgcgcttggc atcatggtgg cgctggaccg cgacaagccc ctgcacgcgc
     3601 tcaggacgat gtcgggggtg ttgcacaagg gcgggtcgct gctgatcttc ccggaggggc
     3661 acctgggcac cgaggagggc aggctcctgg agttgcagga cggcgccgcc cacctgagcc
     3721 agaagtcggg cgtgccgctc ttgcccgtgg gcctgacagg tacgagcaag ctgtggctga
     3781 ggcggaccct cacggtctgt atcggccctc ccatacagcc ccacgacttc acgggcgacc
     3841 tccgcaccag gctccactct atgaccgcct ccctcgacgc tgccatgcgc tccctgctgc
     3901 ccggcgacac cgagcaccca cggctccggc tgctggaacg ctggctgacc aggctgctct
     3961 gatccatcgc gcgtgttgaa ccccacaact cgttcgagcg tattctaagt agatacattc
     4021 gagtaggagc tataaagtta tggggagacc cgcgcgtatt gagccggtgg ggcctgggga
     4081 ggagtcggtg tgggagtacc cgaggccgcc ccgccttgag gacaccgcca agcacatcca
     4141 ggtggtgttc aacggcgtga ccgttgccga caccaggcgg gccaggcgag tgcttgagac
     4201 gagccacccg cccgtatact acatcccgcc cgaggacgtg cttcagagcg ccctggtgcc
     4261 caccacacgc agcacctatt gcgagtggaa gtggcatgcg agctacttca gtgtgcgggt
     4321 gggcgacaag accgccccca acgccgtgtg gagctacccc gacccttcgc ccggcttcga
     4381 ggggatcgcc gggtacctgg cgttctaccc cggcccaatg gacgcatgcc tggtggacgg
     4441 tgagcaagtc caatcgcagc cgggcagctt ctacgcgggt tgggtgacga gcgaaatcaa
     4501 ggggccgttc aagggcgaac cggggtcgag cggctggtag tgggcacggt ggaacgggga
     4561 ggggttaggc gtctcgcagc cagattttag cggccaattc cttcgttagc gacacggtgt
     4621 tggccttggt tcctccggcg gcgtcgcggg ggacaacgct tacgtggaag cttccgtcgt
     4681 cggggtcgcg gtcgagcacc gtgacaagcg cgccgggcat gacgtgcagg ctgcccaggt
     4741 aggtgagcag ggccgtctcg tcttccacca cctcggagat gagcagcacc ttcatcgtct
     4801 tgcccggttc ggcctggctc aagcggatgg ctttctgctc gcgcaggtaa tcgagggtgt
     4861 tggagttctg gccggggatg gggttgccgt gggggcaggt gcgcgggttg ccgaggagct
     4921 tcaccatctg gtcttccagg gccgccgaca tgccgcgctc caggttgtgg gcctcctcgt
     4981 gggcctgcac ccagtccatg cccatcacgt ccaccaggaa gcgctccgcg aggcggtgct
     5041 ggcgcaacat tagctcggcc tgggagcgtc cttcgggggt aagctcgacc ccttcgcccg
     5101 ctttgccccg gccccggccc ggcatcttga tgaggccgtc gcgctccatg cgggtgagca
     5161 cctgggccac gttggggggc gatacgccga acttctcggc cagccgcacg cccacgacgg
     5221 ggtcgccctc tactgtgatg ttgtagatgg cttccaggta ctcgcgagtt acgggcgtaa
     5281 ttgcggacat accctgctcc cccatgcgca cggccagtgc atgcgacctt gtgcgttctg
     5341 cttgcagtat agcggcgtgg ggggagacgg tcaaacgaac cgggcgcgga aagggcgcga
     5401 gggtaaggca gcccgggcgt tcgtgcagtc gcgaccccgc gattgcagca caaggctggc
     5461 ctctgtcatt tcga
//
LOCUS       DASRWZ010000543         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_797945, whole genome shotgun sequence.
ACCESSION   DASRWZ010000543 DASRWZ010000000
VERSION     DASRWZ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797945"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..941
                     /locus_tag="VFH60_08510"
     CDS             264..941
                     /locus_tag="VFH60_08510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6313863.1"
                     /translation="MRIARSNRSLFTRARAALAATLAVAVVAAAVLTPVASQTASART
                     QNAPIRQNVTQFLTLPFEASDSMNILSGWYYSSSGGFHGGIDYINGNVNRVGGWKTFP
                     VIASADGYACGNCTSRQGNAVWVEHKLNGSKYYTYYGHLASIDKGIPLGSQSRTVFVK
                     RGQFLGWAGDTGVGRGVLHLHYALMDANSTPIDPYSIGRLRQYYPSPRQNAPGIGWFM
                     GAAVPSN"
     gene            complement(1370..2299)
                     /locus_tag="VFH60_08515"
     CDS             complement(1370..2299)
                     /locus_tag="VFH60_08515"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET6313864.1"
                     /translation="MQTITDLTQRQPIPAVLSIGSFDGVHLGHQALLGSVVESARARG
                     VAAVALTFEPSPREVLRPDLSLSYLTRLPQKLDILGSLGLDETIVVPFTRELSQVEAP
                     DFIAWLRQYLPFVEMWEGEGFALGHGRTGNTGVLARLGEEMGYTLRIAPLVEIEGEPV
                     SSTRVRQAVAEGDVESAARLLGRPYALPGIVVHGSKRGRELGYPTANLDTPPNQAIPA
                     DGVYASWCVRPATGETLPSLTSIGIRPMFENDVRLVEVYILDYDADLYDETLTTQFVR
                     YLRPQQRFDGLQAFIAQMQKDEADARKLLVGSG"
BASE COUNT          476 a          828 c          749 g          483 t
ORIGIN      
        1 cttctaaact cgaaattcga agttccaatt cccaaattcg actaggatat tctacgtaga
       61 gaggcattgt tacatacgta acgttacgta gcggctgtag taacgttgca gtaatcttgc
      121 cggaatcggc aattttcgct ggatttgtgc ctgagaaggc cggtataatc ggcccgttac
      181 gtcaacttcg ccgctcgctt atgctggcgg ccttgcaaaa gcgcgtcacg acatagctga
      241 ttatagatgg aggtctgttc ttaatgcgta tcgctcgctc caaccgttcg ctctttaccc
      301 gcgctcgcgc cgctcttgct gccaccctag cggtcgcggt cgtcgcggct gcggtcctca
      361 cgcccgtggc ttctcagacc gcctcggccc gcacccagaa cgctcccata cgccagaacg
      421 tcacccagtt cctcacactg cccttcgagg cctcggacag catgaacatc ctgtcgggct
      481 ggtactattc cagctccggc ggcttccacg gcggcatcga ctacatcaac ggcaacgtga
      541 accgcgtggg cggctggaag accttccccg tgattgcctc agccgacggc tacgcttgcg
      601 gcaactgcac ctcgcgccag ggcaacgccg tgtgggtcga gcacaagctc aacggcagca
      661 agtactacac gtactacggc cacctggcca gcatcgacaa gggcatcccg ctcggctcgc
      721 agtcgcgcac cgtgttcgtc aagcggggcc agttcctcgg ctgggcgggc gacaccggcg
      781 tgggcagggg cgtgctccac ctccactacg ccctcatgga cgccaacagt acgcccatcg
      841 acccgtacag catcggcagg ctgcgccaat actatccctc ccccaggcag aacgcccccg
      901 gcataggctg gttcatgggt gccgctgtcc cctccaacta agccacccgt cacaagcaca
      961 agcgcatacg caaagagaga ccccgctgtt cccggcagcg gggtctctct ttcagaagga
     1021 ggaaaggaac ggactagctc ttgtttgctg tctctatgtc gttctgatta agcatacgcc
     1081 tggcgttaca aatcggattt agtggcccac agagggcctt gtcagcctgt attccccttg
     1141 tcatcctgag cgcagcgaag gaccagcccc ccgctctgga ctctcccttg tgccgcaccc
     1201 ctgttccact agctgccccg ccaggaatag tgggctacgt ggaggcgagc cgcacacagt
     1261 gctgtcgctc gatatagcgg atggccgggg ccagggcatc tatatcgtgg tcaaccctgg
     1321 taagctagag cacaccccgc gtatggaata aaaggagcga ctggccttac tagccgcttc
     1381 caaccagcag cttcctggcg tccgcctcgt ccttttgcat ctgggcgatg aaagcttgca
     1441 ggccatcgaa gcgttgctgg ggccgcaggt agcgcacgaa ctgcgtggtg agcgtctcgt
     1501 cgtacaggtc ggcgtcgtag tcgaggatgt agacttccac caggcgcacg tcgttctcga
     1561 acatggggcg gatgccgatg ctggtgaggg agggcagcgt ctccccggtg gcgggccgca
     1621 cgcaccagct tgcgtacacg ccatcggcgg ggatagcctg gttgggcggc gtgtcgaggt
     1681 tggcggtagg gtagcccagc tcccgccctc gctttgagcc atgcaccacg atgccgggca
     1741 gtgcgtaggg gcggcccagc aggcgggcag cgctctccac gtcgccctcg gccaccgcct
     1801 ggcgcacgcg ggtgctgctc accggctccc cctcgatctc taccagcggg gcgatgcgca
     1861 acgtatagcc catttcctcg cccaggcggg ccagcacgcc cgtgttgccc gtgcgcccgt
     1921 gccccagcgc gaagccttcc ccctcccaca tctcaacaaa gggcaagtac tgccgcagcc
     1981 acgcgatgaa gtcgggggcc tctacctgcg acagctcgcg cgtgaacggc accacaatcg
     2041 tctcgtccag gccgaggctg ccgagtatgt ccagcttctg cggcaggcgt gtgagatagg
     2101 agagggacag gtcgggccgc aacacctcac gcggcgacgg ctcgaaggtg agcgctacgg
     2161 ccgccacgcc ccttgcgcgg gcgctctcca ccacgctgcc cagcaacgcc tggtgcccca
     2221 ggtgcacgcc atcgaacgag cctatgctca gcacggcggg tatgggttgt ctttgtgtga
     2281 ggtcggtaat agtttgcatt gtgcaccggg agaaggggta ctgagattat aagctgcacc
     2341 agcaggtgag cgcaaaggcc gcatgtcgcg cgtgagaatg ccttgcccat aacgaagctc
     2401 aacaaatcaa ccaacataag gtgccaggcg acgccccgcg attgaaatcg ggggctacac
     2461 cttgccaagt ccctacggga ctgcctgtgt gcctttgggt agcaatcgat tgaatatgtg
     2521 gcttgaggag tccccc
//
LOCUS       DASRWZ010000544         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1003353, whole genome shotgun sequence.
ACCESSION   DASRWZ010000544 DASRWZ010000000
VERSION     DASRWZ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003353"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..827
                     /locus_tag="VFH60_08520"
     CDS             411..827
                     /locus_tag="VFH60_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET6313865.1"
                     /translation="MLVTKKLSASTRGDGHTIDITGDVERAVKEAGVGSGVVTLFVVG
                     STAGLTTIEFEEGAVIDFARLFEKLAPRDAPYQHELRWHDDNGHSHVRAALLGPSLSV
                     PFVAGKLVLGTWQQIVLVDFDTRPRRREVVAQIMGE"
     gene            1001..1597
                     /locus_tag="VFH60_08525"
     CDS             1001..1597
                     /locus_tag="VFH60_08525"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET6313866.1"
                     /translation="MTDIEVFVGEVETPAGVFGAALTSRGLGRLIFPGEPFEQCEAWV
                     ARWEPGAPRSGDRTGLLRLGEQLTGYFEGTLREFTVPLDMRGTPFQLRVWSALLDVGY
                     GELRTYGQIASGMESPKAVRAVGAANGANPVPIIVPCHRIIGSSGRLIGYGGGLDMKR
                     RLLELEGVMSAFEPSLPLFAGPTRLSSLPSLSRQSAPA"
     gene            1720..2523
                     /locus_tag="VFH60_08530"
     CDS             1720..2523
                     /locus_tag="VFH60_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET6313867.1"
                     /translation="MRRLARLGSTPALVEFEFDANGEELRTKAVWEHAGVGPGRLSTA
                     RLECLACASWGLEDDLPACYAHLGKEPQLAPVVARYHGMRLARVPDLYEALLVAILGQ
                     QISVRAANACRARLMASLGESLHFEGSKYTTYPLPARLLAAGAEELRTLGTGRQKARY
                     LLSLAQAALVGNLAGAKFQDLSDDEATALLCEIPGVGRWTAEVALLRGLGRLDVFPAG
                     DLGLQVAAQRVFGLARRPSERELRLMAERWRPWRGYAAVYLWAYLRDTM"
BASE COUNT          523 a          829 c          973 g          537 t
ORIGIN      
        1 aactcgggcg agtcgaaact ggtggagcta atagtgcggg ccaacgtcgc actgtaaagg
       61 cccaggctga gcagggtaac tagggcagcc acaatccagt ccacaaaccc ccatgacctg
      121 cgcgcgcggg acggcaggta cggaacatac ggaatgtgtg gaggggagtg gtgtgccggg
      181 gaggccgggg tgctatgtag aactggcaat gacatatttg agaaacgttc cacacgcatt
      241 ataggtttgc gtgactcgcc gtgtcaaaat tctgttgcgg tagagcgtgc ctggacgcgt
      301 gcctcgctgc ctattgttca cgaatgttga gggcagcaag gtggtgttgt atacgcgacc
      361 tgggacgccc ggacgtggta gtatagcggg gcggcaggag aggttggaag atgttagtca
      421 ctaagaagct gagcgcaagc acgcgcggcg acgggcacac aatcgacatc acgggcgacg
      481 tagaacgggc ggtgaaggaa gcgggcgtcg gcagcggggt agtgaccctc ttcgtcgtgg
      541 ggtccaccgc cgggctgacg acaatcgagt tcgaggaggg ggcggtaatc gactttgccc
      601 gcctgttcga gaagttagcg ccccgcgacg ccccttacca gcacgaactg aggtggcacg
      661 acgacaacgg ccacagccac gtgcgggcgg cgctgctcgg cccgtcgctt tcggtgccct
      721 tcgtcgcggg caagctggtc ctcggcacct ggcagcagat cgtgctggtg gacttcgata
      781 cgcgcccgcg caggcgagaa gtggtcgccc agatcatggg agagtgacac ggcagggcat
      841 ggccggtgtt gcgattacgt tgcgtcggcc ggaatgtagc ttaagacagc aagattccgg
      901 gagattccac gctctccgct gtgctaacat agtggaatag ccggtgcgga gtcctgggtt
      961 gtcaggtgga ctgtggttta tatttgcagg gagcaaaaca gtgacggata tagaggtatt
     1021 cgttggagag gtagaaacgc ccgccggggt gttcggggcg gcgctcacgt cgcgagggct
     1081 tggccgcctg atatttcctg gggagccgtt cgagcaatgc gaagcctggg tggcgcggtg
     1141 ggagcctggt gcgcctcgct cgggagaccg caccggcttg ctccgactgg gagagcaact
     1201 gaccgggtac ttcgagggca ccctccgaga attcaccgtg cccctggaca tgcgcggcac
     1261 ccctttccag ctacgggtat ggtccgccct gctcgacgtg ggctacggtg agctacgcac
     1321 ctacgggcag atcgcctccg gcatggagtc acccaaggcg gtgcgggcag tgggcgcggc
     1381 caacggtgcc aacccggtgc ctatcatcgt gccctgccac cgtatcatag ggagcagcgg
     1441 gcggctaatc gggtacggcg ggggccttga tatgaaacgc cgcctcctgg aactggaagg
     1501 cgtaatgagc gcgttcgagc cgtccctgcc cctgtttgcc ggcccaacga ggctgtcctc
     1561 tctgccttcg ctttcgcgcc agagtgcccc agcttagcga cacaggtgga aatcagtctg
     1621 cttcggctgc cgcagccatt cgatgttggc ctatcgctgg atagcctgca acggtcttca
     1681 tacggtgcgc cctacaattt ttccaccggt ggccgggccg tgcggcgact ggcgcgcctg
     1741 ggtagcaccc cggcgctggt agaattcgag ttcgacgcga acggcgagga actgaggaca
     1801 aaggcggtct gggagcacgc tggtgtaggc cccggtcggc tgtctacggc gcggcttgag
     1861 tgcctggctt gcgcgtcatg ggggctggag gacgatctgc ctgcctgcta cgcgcatctc
     1921 ggcaaggagc ctcaactcgc ccctgtggtc gcccgctacc acggcatgcg gctggcgcgg
     1981 gtgcccgatc tgtacgaggc gctgctggtg gcgatactgg ggcagcagat cagcgtgcga
     2041 gccgccaacg cctgccgtgc gcgcctgatg gcctctctcg gagagagcct ccacttcgag
     2101 ggcagcaagt acacgacata tcccctacca gcacgcctcc tggccgccgg tgcggaggag
     2161 ctgcggacgt tgggcacggg cagacagaaa gcccgctatt tgctctcgct ggcgcaggcg
     2221 gcgctcgtgg ggaacctggc cggcgcgaag ttccaggacc tctccgacga cgaagccaca
     2281 gccctgttgt gcgagatacc cggagtgggc aggtggacgg cggaagtggc cctgttgcga
     2341 ggactgggac gcctggacgt ctttccggcg ggcgacctgg ggttgcaagt ggcggcccaa
     2401 cgcgtgttcg ggcttgccag gcggccctca gagcgcgagc tacgcctcat ggccgaacgc
     2461 tggcggccgt ggcggggcta tgcggctgtc tacctctggg cttacctgcg cgatacgatg
     2521 tgatggaagc aaggcaagaa ggggagtatg ggaaatagat tggtgagtct ccccctgtca
     2581 tcctgagtgg agcatcttgt aagtaagtta ccaataggag gaccaaatac cacctgtcat
     2641 cctgagcgta gcgaaggatc agtagcccgt gtttgaccct tcccattgag gcaccactgt
     2701 tcctaatgcc atcatcatcc ccaatgttga agactctcgc ctacccacct gatccttcgc
     2761 tccgctcagg atgacagggc agcttttagt cgccagactc ctcgcgccgt gcccttcggt
     2821 tcgcatcagg agtcgtacat tacgtgtcac tcgtaacccc tc
//
LOCUS       DASRWZ010000545         7414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1140989, whole genome shotgun sequence.
ACCESSION   DASRWZ010000545 DASRWZ010000000
VERSION     DASRWZ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140989"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1095)
                     /locus_tag="VFH60_08535"
     CDS             complement(157..1095)
                     /locus_tag="VFH60_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET6313868.1"
                     /translation="MRKISTLLAALVIVMSLAACDPGGGSTGGDLSQRKIKVTTTVGM
                     IKDIVVNVGGERVEAAGLMGPGVDPHLYKPSASDVQKLEEADVIFYGGLELEGRMTDT
                     FVRIARSGKATVAVSEGIDPARLREPPEFHGKYDPHIWFDVLLWQEAVKAVHQELARL
                     DPASKDLYQKNTDAYLKQLDELHNYVKEQIASIPKESRVLITAHDAFGYFGQQYDVEV
                     KGLQGTSTATEAGVGDVQGLANLIVERKIKAIFVESSVPQATIEAVQEAARSKGWDVN
                     IGGTLFSDAMGNDGTPEGTYVGMVRHNVDTIVKALK"
     gene            1285..2001
                     /locus_tag="VFH60_08540"
     CDS             1285..2001
                     /locus_tag="VFH60_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET6313869.1"
                     /translation="MRDRIGPDLHIGRLMVSHAIEDYLKAIYRLQQDGSAATTNAIAE
                     RVGVRAASVTSMLQQLSEHGLAQYTPYKGVYLTGDGLALALRVIRRHRLIELYLHEKL
                     GVPLERLHAEAERLEHAISPYLEERIDMALGYPNFDPHGDPIPSTLGEMPVRELIPLN
                     ELPTDTPGVVRHIPDDDPDVLRYVASLGLLPDATVTITRREPFDGPVSLRIAARDGKP
                     DNEQVVSTAMARLIYVSSPA"
     gene            2236..2436
                     /locus_tag="VFH60_08545"
     CDS             2236..2436
                     /locus_tag="VFH60_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET6313870.1"
                     /translation="MRSYCVYILASASRTLYVGVTNNIVRRVYEHKCKKADSFTARYN
                     INRLVYFAEGRDVRDAIARERD"
     gene            2654..3148
                     /locus_tag="VFH60_08550"
     CDS             2654..3148
                     /locus_tag="VFH60_08550"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET6313871.1"
                     /translation="MAKLNPLTRLLDWIVLEGSKRLGKGTTERHVAIYRKTGGLLGGR
                     IRELPVLLLTTTGRKSGEPRTQPLNYLRYGTAYVVAASNSGRDSYPAWFLNLQSNPRA
                     SVQVRRKVVPVVARVATEEEHARLWVELTSKARNYAAYTRMTERDIPMLILQPEARGK
                     EQNG"
     gene            3141..5432
                     /locus_tag="VFH60_08555"
     CDS             3141..5432
                     /locus_tag="VFH60_08555"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HET6313872.1"
                     /translation="MADKKHISFGMVLHNHQPVGQSDQVLEEIYEKSYEPQVAALERH
                     PGVRVGLHYTGSLLDWLRQHRPEYVQRVRALVARGQVEVLSGGYYEPVLPSIPDDDKV
                     SQVLKLNRAVLDDFGYNPTGMWLAERVWEPTLPTYLSRGNIAWTIVDDVHFKMVGLTD
                     DDVTGYFVTENNGDAVNVFGTSKILRYNIPWQPVEETISFLRDETQPTPGKILVIGDD
                     GEKFGAWPGTFEHCWGTNGQNGWVDDFFSALEANAAWLHCITLGDHVRNFPAIGRVYL
                     PTASYSEMMEWALPARQSHELNQLMHGIGEAKTEPAHAKFMRGGFWRTFLVKYDEINS
                     QHKKMLRVHDKIQQGRWLANERGNDLAQYGDFGQEDLWRGQSNDTYWHGLFGGVYMTD
                     IRARIYGHLIRAQKAAERGIYGDQAWLSHEIVDFDRDSMQELLVEGSALDLYFDLADG
                     GTIFEWDLRKHFYNLASTLTRREEAYHSTLKEYEEKRRFQPPAKSGKKKAAKSTSSAK
                     GDVAADKEGVESPHESVKVREPDLDRYLDYDSYRRACMVDHFLGPGTTLEGFMSGRYN
                     EEGDFVNGAYNAELQEGGLRILHVMLERDGFITTEAGRRPVRVSKRLSLRPGSSDFRV
                     LYTVQNTGDDDVAGVFASEWNLNLLGGGRNPSAYYRVEGVELEDASLESIGETRNVQE
                     LAVGNSWLGIEMTLRSNVEATLWRFPIYTVSGSEAGFERTYQGNCVLLQWLFKLPPSE
                     SVDIELEWVYTGSGVNNPDFLTE"
     gene            5468..6616
                     /locus_tag="VFH60_08560"
     CDS             5468..6616
                     /locus_tag="VFH60_08560"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6313873.1"
                     /translation="MAFDARMVHYRRAGGIGQYSVSLLRALSRLPEVGRGARVQVLQM
                     RGDSARIVRDRRFRRVPVWTPPHHRFEQPALGVELLKLWPRPQLVHSPDFVPPRYRTM
                     RAVANIQDLAFLKFPGLTLLTDESKRYYGQVHRAAQDADALIALSHSARDDIESLLGV
                     KPEKVAVIPAAAGEQFKPPDDFRHAQELTAREFGLPAPDEGGYVLFVGTIEPRKNLPT
                     LLEAYRVLLDAGRVSPAPALAVAGREGWLFEQVHARIDELRLSGHVRLLGGVSDAALV
                     RLYQGAQAFAMPSLYEGFGLPVLEAMRSGVPVVSSNAGSLAEVAGDAAILVEPQDVDG
                     WAAGLERVLLDEGEAARLVAAGLRQAGRFSWERAARETWDLYGRVVSG"
     gene            complement(6613..7197)
                     /locus_tag="VFH60_08565"
     CDS             complement(6613..7197)
                     /locus_tag="VFH60_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HET6313874.1"
                     /translation="MDVFTLILSGFGLAAPAGLNAWLPLLITGLAARFHLFGLTLNAP
                     FNLLTETWVLIIIGVLLTIEVFADKIPAVDTVNDIIHTFIRPVAGGILFAAHSGAVGG
                     MDPTLSFILGMLSAGSVHAVKATSRPVITATTGGIANPLVSMVEDLVAAGATVLALAA
                     PLVAAVLMGSLLAFAAWMVWRWRQRRKRRRAAQI"
BASE COUNT         1386 a         2223 c         2439 g         1366 t
ORIGIN      
        1 atcccggtag aggacgagat tcttcgctgc gctcagaatg acaaaggcca acagggtccc
       61 tccatgacac cagtgtagca gtcacgcatc ggctagggta ggtcgcatta ctagccactc
      121 agtcctcagt cctcagtcct cagtcctcat cactcgtcac ttcaacgctt tgactatcgt
      181 atcgacgttg tgccgcacca tgccgacgta ggtgccttct ggggtgccgt cgttgcccat
      241 cgcgtcggag aagagggtgc cgccgatatt gacgtcccag cccttcgagc gggctgcctc
      301 ctgcaccgcc tctattgtgg cctggggcac ggaggactct acgaagatag ccttgatttt
      361 gcgctcgact atcaggttgg cgaggccttg cacgtcgccc acgcccgctt cggtggcggt
      421 gctggtgccc tgcaagccct tcacctccac gtcgtactgc tggccgaagt agccgaaggc
      481 gtcatgggcg gttatcagca cgcgggactc tttcggtatg gaggctatct gctccttgac
      541 gtagttgtgc agctcgtcca gttgcttcag gtatgcgtcg gtgttcttct ggtagaggtc
      601 tttcgatgcg gggtcgagcc tggccagctc ctggtgtacc gccttgaccg cttcttgcca
      661 caggagcacg tcgaaccaga tgtggggatc gtatttgccg tggaactctg gcggctcgcg
      721 cagcctggcg gggtctatgc cctcactgac ggccacggtg gctttgccgg agcgggcgat
      781 gcgcacgaag gtgtcggtca tgcggccctc aagctccagg ccaccgtaga agatgacgtc
      841 ggcctcctcc agcttctgca cgtcgctggc gctcggcttg tagagatggg ggtctacgcc
      901 gggacccatc aaccccgccg cttccacgcg ctctccgccg acgttgacca cgatatcctt
      961 gatcataccg accgtggtgg ttactttgat cttgcgttgc gagaggtcgc cgccggtgct
     1021 tccgccgccg gggtcgcaag ccgccagcga catgactatt acgagcgcgg cgagtagagt
     1081 gcttatttta cgcatctgct atttttctcc tggtgtccgt gattgtatag cttcgtagat
     1141 tgtgatatac tgaaaccata ttttagatta gcctaaatac cctcccagga tacaccaaaa
     1201 tgggccgctt gtcaatagct tggaagaccc aattcgccga attttagggt ggtctaaaaa
     1261 tcttcggagt tgttccggcg cacggtgcgt gatagaatag gcccggacct gcacataggg
     1321 agactcatgg taagccacgc gattgaggac tacttgaagg ccatataccg gctgcaacag
     1381 gacggctcgg cggccaccac aaacgctata gccgagcgcg tgggggtgcg cgcggcttcc
     1441 gtcacctcga tgctacagca gctctccgag catggtctgg cccagtacac gccgtacaag
     1501 ggggtctatc tcaccgggga cgggctggcg ctggcgttgc gggtgatacg ccggcacagg
     1561 ctgatagagc tatacttgca cgagaagctg ggcgtgcccc tggagcggct gcacgccgag
     1621 gccgagcggc tggaacacgc catctcgccc tacctggaag agcgcatcga catggcgctc
     1681 ggctacccca acttcgatcc ccacggcgac ccgattccca gtacgttggg cgagatgccg
     1741 gtgcgcgagt tgatacccct caacgagcta ccgaccgata cccccggcgt ggtgcgccac
     1801 attcccgacg acgaccccga tgtgttgcgc tacgtggcct ccctcggcct gctccccgac
     1861 gccacggtga cgatcacacg ccgcgagcct ttcgacggcc ccgtgtcgtt gcgcatagcc
     1921 gcccgcgatg gcaagcccga caatgaacag gtggtcagca cggcgatggc ccggctgata
     1981 tacgtcagca gcccggctta gtggtcagcc gcttactggc ctcccttccc tctgcattcc
     2041 agggttctaa ggcaccgaaa ttgcggtaag acgccttgcg ctatgtggaa tcaaagttgt
     2101 gccgggtggc aagtatatcg gatggtggga ccaagcaccc ctttgtcatt ctgagcgcag
     2161 cgaagaatct cgtccttcac cgggatgttt cacctttctt gatcgctaag agtgcaccct
     2221 cttcaggagt tacacatgag gtcctattgc gtgtacatcc tggcaagtgc atcacgcacc
     2281 ctctatgtgg gtgtcaccaa caacattgtg cgccgggtat acgagcataa gtgcaagaag
     2341 gccgacagct ttacagcccg ctacaacatc aaccgtcttg tctactttgc agaaggtagg
     2401 gacgtaagag atgctattgc gagagaaaga gattaagggt tggctgcgag ctaagaagat
     2461 agcacttatt gaggagatga acccgacctg ggatgatttt agcgaaggtt ggtacgataa
     2521 cagtagttcg tttacggaac atcccggtga gggacgagat tcttcgctac gctcagaatg
     2581 acaaaggggc agagggtccc gcatttggag catcgatgct ttctgtacaa catgcacgag
     2641 gggtgatata gaattggcta agctcaaccc attgacgcgt ctgctcgact ggatcgtcct
     2701 ggaggggtcc aagcgcctcg gtaagggaac tactgagcgg cacgtagcga tttaccgcaa
     2761 gaccggcggg ttgctgggcg ggcggatacg cgaattgccg gtgctgctcc ttacgacaac
     2821 agggcgcaag agcggcgagc cgcgcaccca gccgctcaac tacctccgct atggtaccgc
     2881 ctacgtggtc gcggcctcca attcagggcg cgacagctac ccggcatggt tcttgaactt
     2941 gcagagtaat ccgcgtgcga gcgtgcaagt gcggcgcaaa gtggtaccgg tggtggccag
     3001 ggtggcgacg gaggaggagc acgcgcggct ctgggtggag cttacgtcta aggctcgcaa
     3061 ctacgccgcg tataccagga tgaccgagcg ggacattccg atgcttatac ttcaacccga
     3121 agcgaggggc aaggagcaga atggctgaca agaagcacat ctcgttcggc atggtgctgc
     3181 acaaccacca gccggtaggg cagtcggacc aggtgcttga ggagatatac gagaagtcct
     3241 acgagccgca ggtggccgcg ctggagcgtc accccggcgt gcgggtgggc ctgcactaca
     3301 ccgggtcgct gctcgactgg ctcaggcagc acaggccgga gtacgtgcag agggtgcggg
     3361 cgctggtggc gcgcgggcag gtggaggtgc tttccggcgg ctactacgag ccggtcctgc
     3421 cctccattcc cgacgacgac aaggtgtcgc aggtcctcaa gctcaaccgt gccgtgctgg
     3481 acgacttcgg ctacaacccc accggcatgt ggctggccga gcgcgtgtgg gagccgacgc
     3541 tgcccaccta cctcagccgg ggcaacatcg cgtggacgat tgtggacgac gtgcacttca
     3601 agatggtggg cctcaccgac gatgacgtga ccggctattt cgtcaccgag aacaacggcg
     3661 atgcggtgaa cgtgttcggc acctcaaaga tcttgcgcta caacatcccc tggcagccgg
     3721 tcgaggagac gatcagcttc ctgcgcgacg agacgcagcc cacgcccggc aagatactgg
     3781 taataggcga cgacggcgag aagttcggtg cgtggccggg cacgttcgag cattgctggg
     3841 gcacgaacgg gcagaacggc tgggtggacg acttcttctc ggcgttggag gcgaacgctg
     3901 cgtggctgca ctgcatcaca ctgggcgacc acgtccgcaa cttccccgcc ataggccgcg
     3961 tgtacctgcc gaccgcctcc tacagcgaga tgatggagtg ggcgctgccc gcccggcagt
     4021 cgcacgagct gaaccagctt atgcacggga taggggaggc caagaccgag cctgcgcatg
     4081 caaagttcat gcggggcggc ttctggcgca ccttccttgt gaagtacgac gagatcaact
     4141 cgcagcacaa gaagatgctg cgcgtgcacg acaagattca gcagggccgc tggctggcga
     4201 acgagcgcgg gaacgacctg gcgcagtacg gcgatttcgg gcaggaggac ctgtggcgcg
     4261 gccagagcaa cgacacctac tggcacgggc tgttcggcgg cgtctacatg accgacatac
     4321 gcgcccgcat ctacggccac ctcatccggg cgcagaaggc agccgagcgc ggcatctacg
     4381 gcgaccaggc ctggctcagc cacgagattg tcgatttcga ccgcgactcc atgcaagaac
     4441 tgctggtgga gggcagcgcg ctcgatctct acttcgacct ggccgacggc ggcaccatct
     4501 tcgagtggga cctgcgcaag cacttctaca atttagcctc caccctcacc cgccgcgagg
     4561 aagcctacca ctccacgctc aaggaatacg aggagaagcg ccgcttccag ccccccgcga
     4621 aatccggcaa gaaaaaggcc gctaagtcca ctagctcagc gaaaggcgac gttgccgctg
     4681 acaaggaagg ggtggagtcg ccgcacgaat cggtgaaggt gcgcgagccg gacctggacc
     4741 gctacctcga ttacgactcc taccggcgcg cctgcatggt ggaccacttc cttgggccgg
     4801 gcaccacgct ggaaggcttc atgtcggggc ggtacaacga agaaggcgat ttcgtcaacg
     4861 gggcctacaa cgcggagcta caggagggcg gcctgcgcat actgcacgtc atgttggagc
     4921 gcgacggctt catcacgacc gaggcgggtc gcaggccggt gcgggtgagc aagaggctgt
     4981 cgctccggcc cggctcgtcc gatttccgcg tgctctacac ggtgcagaac accggcgacg
     5041 acgacgtggc gggtgtgttc gcctcggagt ggaacctcaa cctgctgggt ggcgggcgca
     5101 acccctccgc ttactacagg gtcgaagggg tagagctgga ggatgcgtcg ctggagtcga
     5161 ttggcgagac gcgcaacgtg caggagctag cggtgggcaa ctcctggctg ggtatcgaga
     5221 tgacgttacg ctcgaacgtc gaggctaccc tgtggcgctt ccccatctac accgtgtcag
     5281 ggtcggaggc gggcttcgag cgcacctacc agggcaactg cgtgctgctg caatggctgt
     5341 tcaagctgcc gccgagcgag tcggtggaca tcgagctgga gtgggtctac accggcagcg
     5401 gcgtgaacaa ccccgatttc ctgacggaat gatttgggaa ttgggaattg gaattgcgga
     5461 tttgagggtg gcgtttgacg ctcgtatggt gcactaccgg cgggcgggtg ggatagggca
     5521 gtatagcgtc agtttgttgc gggcgctatc gaggctgccg gaggtggggc ggggtgcgcg
     5581 ggtgcaggtg ctccaaatgc ggggcgacag tgcgcgcatc gtgcgggacc gccgcttccg
     5641 ccgcgtgccg gtgtggacgc cgccccacca ccgcttcgag cagcccgcat tgggcgtgga
     5701 actgctcaag ctgtggcccc ggccgcaact ggtccactcg cccgacttcg tgccgccccg
     5761 ctaccgcacc atgcgcgccg tcgccaacat tcaggacctt gccttcctca agttccccgg
     5821 cctgacgctg ctcaccgacg aaagcaagcg gtattacggc caggtgcacc gcgctgcgca
     5881 ggatgccgac gccctgatag ccctctccca ctcggcccgc gacgatatag agtccctgct
     5941 gggggtgaag ccggagaagg tggcggtcat cccggcggcg gctggggagc agttcaaacc
     6001 gccggacgac ttccgccacg cccaggagct tacggcccgc gagttcggcc tgcccgctcc
     6061 cgatgaaggc ggctacgtcc tcttcgtggg cacgatagag ccgcgcaaga acctgcctac
     6121 gctgctggaa gcctaccgcg tgctgctgga cgcggggcga gtgtcgccgg cccccgcgct
     6181 ggcggtcgca gggcgcgagg gctggctctt cgagcaggtg cacgccagga tagacgagct
     6241 gcgattgagc gggcacgtgc ggctgctggg cggcgtgagt gatgccgcgc tggtgaggct
     6301 ctaccagggg gcgcaggcct ttgccatgcc gtcgctgtac gaggggttcg ggctgccggt
     6361 gctggaggcc atgcggagcg gggtgccggt ggtctcctcc aacgccgggt cgctcgcgga
     6421 ggtggcgggc gatgcggcca tactggtgga gccgcaggac gtggacggct gggcggcggg
     6481 cttggagcgc gtgctgctgg acgaggggga ggcggcgcgg ctggtggcgg cggggttgcg
     6541 gcaggcgggg cgattttctt gggagcgcgc ggcgcgggag acgtgggatt tgtatgggag
     6601 ggtggttagc ggctagatct gcgcggctct tctcctcttc ctccgctgcc gccagcgcca
     6661 gaccatccag gccgcgaacg ccagtaacga ccccatgagc acggcagcca ccaggggcgc
     6721 ggccagggcc agcacggtcg cccctgcggc cactaagtcc tccaccatgc tgaccagggg
     6781 gttggctata ccgccggtgg tcgccgtgat gacggggcgc gaggtggctt tgaccgcgtg
     6841 cacgctgccc gccgacagca tgcccaatat gaacgacagc gtagggtcca tgcctcccac
     6901 ggccccgcta tgcgcggcga acaggatacc acccgcgaca gggcggatga aggtgtggat
     6961 gatgtcgttg acggtgtcta cggcgggtat cttgtcagcg aatacctcga ttgtgaggag
     7021 cacgccgatg atgatcagca cccaggtctc ggtgagcagg ttgaaggggg cgttgagggt
     7081 gaggccgaac agatggaacc tggcggcgag gcccgtaatg aggagtggca gccaggcgtt
     7141 cagcccggcg ggtgccgcca gcccgaagcc cgacaagatg agggtaaaaa cgtccatagt
     7201 gccctctact ttaccagatt ccgtaccacc gaatacgccc acgtcgccgg acggtcccgc
     7261 ctgacgagca tgccgcgcac ccaggccgct agctgtagcc ggttgcgcgg cagcgcgcgt
     7321 gaccagtctc tattacgcaa cagataggcg atttgttacg tgtctttcac tgcgccaggt
     7381 acgttgcaag ctatctcaag agtgacgatg tgga
//
LOCUS       DASRWZ010000546         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1003728, whole genome shotgun sequence.
ACCESSION   DASRWZ010000546 DASRWZ010000000
VERSION     DASRWZ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003728"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFH60_08570"
     CDS             <1..443
                     /locus_tag="VFH60_08570"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET6313875.1"
                     /translation="ALQNRGHGFSMERGHPNFVRPGKRPYHTIIPGFLTRDGQAVGPY
                     GVMGGFMQPQGHMQMVVNLVDYGMNPQASLDAPRWQWARGRTVQVESNASPAIIEGLR
                     QRGHDIEVAEPSGGFGRGQIIWRLPSGAYAAGSDKRADGYAAGI"
     gene            complement(522..1049)
                     /locus_tag="VFH60_08575"
     CDS             complement(522..1049)
                     /locus_tag="VFH60_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET6313876.1"
                     /translation="MDTMERRYTKADLLNAIEGAWTNLNAALDRLTQEQMTETRDPQG
                     WAVKDHLVHIAAWERSVVVFLQGRPRHEGLEVDEQVYARGDDDEINAAIQEKRKDVSL
                     SEALDDMRTVHGQLLSLLEPMTDDDLYKANSDFQPNATGERDERPVAGMIYSNTANHF
                     SEHQEWIESLVSRQS"
     gene            complement(1068..1712)
                     /locus_tag="VFH60_08580"
     CDS             complement(1068..1712)
                     /locus_tag="VFH60_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET6313877.1"
                     /translation="MFSDVEGTLVDASLPRLSLQVGSRVGVFPRWKIAELAVYGLLAK
                     VLPSKYQRRMRFYIIKRAMVGRTVAEVARLCEAVTPRVVASLKQGNWARVKRHRDEGL
                     PLVLMSAGLHEVIARLGQELGARGEGTHFVVRGGRYLARFDGPICEGTGKRDRALSIA
                     RELGCDPAQCYAYGDTTADIPFLEAFGHPCAVDPDAELEAEATRRGWEIIRSTS"
     gene            complement(1739..2617)
                     /locus_tag="VFH60_08585"
     CDS             complement(1739..2617)
                     /locus_tag="VFH60_08585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6313878.1"
                     /translation="MLTGVVFPQTEIGDDPTKVRDYGQAAEQLGYSHILAYDHVLGAS
                     TKNRPDWRGPYTSDTMFHEPLVLFAYLAGITQKVEFVTGVIILPQRQTALVAKQAAEL
                     DVLSGGRLRLGVGIGWNDVEYEGLNENFRNRARRFEEQIEVLRLLWTQPVLDFHGQYH
                     NITEAGINPLPVQRPIPLWIGANVAAGVERAGRIADGWFPQVPPDERGREMVGIFRSA
                     AEQAGRDPNSLGIEARISLAQGGPDDWRQRYESWRDLGATHMSINTMGMGLASPDEHI
                     SAIERFKREVVDVGNW"
BASE COUNT          455 a          882 c          833 g          532 t
ORIGIN      
        1 tcgcgctaca gaaccggggc cacggcttca gcatggaacg ggggcacccc aacttcgtgc
       61 ggcccggcaa gcgcccctac cacactatca tcccaggctt cctcacgcgg gacggccagg
      121 ctgtggggcc gtacggggtc atgggaggct tcatgcagcc ccaggggcac atgcagatgg
      181 tagtgaacct tgtggactac ggcatgaacc cacaggccag cctggacgca ccacgctggc
      241 agtgggccag gggacgcacc gtgcaggtcg agtcgaacgc cagcccagcc ataatagagg
      301 gcctgaggca gcgggggcac gacatagagg tagccgagcc gtcgggtggg ttcgggcgcg
      361 ggcagatcat ctggcggctg ccgtcagggg catacgccgc tggcagcgac aagcgggcgg
      421 atggctacgc ggcggggatt tagctcgatt cgttatcctg ggtgatatag gggctaccac
      481 catcatggtg gtagccccta tatctgcaat caagccgtcc tctagctctg tcgtgagacc
      541 aggctctcga tccactcctg gtgctcgctg aagtggttgg cggtgttgct ataaatcatg
      601 cctgctacag ggcgctcgtc tcgctctccg gtggcgttgg gctggaagtc gctatttgcc
      661 ttgtacaggt cgtcgtcggt catcggctcc agcaggctga gaagctggcc gtgtacagtg
      721 cgcatatcgt ccagcgcctc ggagagcgac acatccttgc gcttctcctg gatggcggcg
      781 tttatctcgt catcgtcacc cctggcgtag acctgctcgt ctacctcaag cccctcgtgg
      841 cgtggcctgc cttgcaggaa cactacaacc gaacgctccc aggccgctat atggaccagg
      901 tggtctttca cggcccagcc ttgcgggtcg cgggtttcgg tcatctgctc ctgcgtcaag
      961 cggtccaggg cggcgttgag atttgtccag gccccttcta tcgcgttcaa caggtccgct
     1021 ttggtatatc gccgttccat cgtatccatc tctcctccaa ttgaattcta actagtgctt
     1081 ctaattatct cccacccccg cctcgtggct tcggcctcca actccgcatc ggggtcaacc
     1141 gcgcaggggt ggccgaaggc ttctaggaag gggatgtcgg cggtggtgtc gccgtaggcg
     1201 tagcactggg cggggtcgca gcctagctcg cgggcgatgg agagggcgcg gtctcgcttg
     1261 ccggtgccct cgcagatggg gccgtcgaac cgggccaggt agcggccgcc gcgtaccacg
     1321 aagtgggtgc cctcgccgcg agcgcctagc tcctgcccca gccgcgctat gacctcgtgc
     1381 agccccgcgc tcatgagcac gagcggcagg ccctcgtccc gatgcctctt gacccgcgcc
     1441 cagttgccct gcttcaagct cgcgaccaca cggggagtga ccgcctcaca aagccgcgct
     1501 acctccgcca cggtgcgccc caccatcgcc ctcttgatga tgtagaagcg catccgccgc
     1561 tggtactttg acggcagcac cttcgctagc agcccgtaca ccgccagttc cgctatcttc
     1621 cagcgcggaa agacccccac gcggctgccc acctgcaacg acagccgggg gaggctggcg
     1681 tccacaagcg taccctcaac gtcactaaac actgctgctt tcataggaca attacgaatt
     1741 accaattacc gacatcgacc acttcgcgct tgaaccgctc tatcgcgcta atatgctcgt
     1801 cgggagaggc gaggcccata cccatcgtgt tgatgctcat gtgcgtggct ccaaggtcgc
     1861 gccagctttc gtagcgctgc cgccagtcgt ccgggccgcc ctgggccagg ctgatgcggg
     1921 cctcgatgcc caggctgttg gggtcgcggc ctgcctgttc ggctgcgctg cgaaatatcc
     1981 caaccatttc gcggccccgc tcgtccggtg gcacctgggg gaaccagccg tcagctatgc
     2041 ggcccgcccg ctccaccccg gcagcgacgt tcgcgcctat ccagaggggg atggggcgtt
     2101 gcacgggcag gggattgatg cccgcctcgg tgatgttgtg gtactggccg tggaagtcca
     2161 agacgggctg ggtccacagc aggcgcagca cctctatctg ctcttcgaac cgccgggcgc
     2221 gattgcggaa gttttcgttc agcccttcat attccacgtc gttccagccg atgccgacac
     2281 ccagccgcag ccgcccgccg ctcagcacgt ccaactccgc tgcctgcttg gcgaccaggg
     2341 ccgtctgccg ttgcggcagg ataatcacgc ccgtgacaaa ttcgaccttc tgcgtgatac
     2401 cagccaggta ggcgaacagc accagaggct cgtggaacat cgtgtcgctg gtgtacggcc
     2461 ctcgccagtc ggggcggttc ttcgtgctcg cgcccagcac gtggtcgtac gcgaggatgt
     2521 gcgagtagcc aagctgctcg gcggcctggc cgtagtcgcg cactttggtg gggtcgtcgc
     2581 cgatttcggt ctgggggaac actactccgg tgagcatagc acgctcctaa ttaggttgaa
     2641 cacgtaacac gtaaagcgta atgcgtaaca cttattagct atgaatgacc tgttacgtgt
     2701 ta
//
LOCUS       DASRWZ010000547         4305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_593258, whole genome shotgun sequence.
ACCESSION   DASRWZ010000547 DASRWZ010000000
VERSION     DASRWZ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593258"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /gene="aroF"
                     /locus_tag="VFH60_08590"
     CDS             <1..696
                     /gene="aroF"
                     /locus_tag="VFH60_08590"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET6313879.1"
                     /translation="KIKRAGAFKPRTSPYSFQGMGEEGLQILADVRESLGMSIITEVM
                     APDEVALVSRYADILQIGTRNMQNYRLLEEVGSCGRPVLLKRGMSSTIEELLLAAEYI
                     LANGNSQVMLCERGIRTFERATRSTFDLNAIPVLKQLSHLPVIADPSHGTGRWDLVTP
                     VSLGAVAAGADGLIIEVHSHPELALSDGGQSLRPDKCRALLDDLGRIANAVGKGLDLA
                     SPVVAGAEAVHAA"
     gene            686..1537
                     /locus_tag="VFH60_08595"
     CDS             686..1537
                     /locus_tag="VFH60_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET6313880.1"
                     /translation="MRLSEATVGIHGLGLMGGSLGLALGGKCKRRIGIVRRPETAELA
                     LQIGAVEAATLDLQAGASAADIVVLATPARRIVMDVEEAGRHMRPGSVLMDLGSTKGA
                     VVEAMDRLPGHVSAVGGHPMCGKEVGGLEHAEAALFHDKVFVLTATARTDEAALTLSH
                     ERVEAVGAHPVLMEPGQHDRAVAYVSHLPYLMAAALVRTEKEAAGCDPTLDRLAASGF
                     RDTSRLAASEVPMMRDILLTNRHAIEEALASFEGQLDTVRRLLDDEAALEGWMLDAQR
                     ERRAMFV"
     gene            1534..2859
                     /gene="aroA"
                     /locus_tag="VFH60_08600"
     CDS             1534..2859
                     /gene="aroA"
                     /locus_tag="VFH60_08600"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET6313881.1"
                     /translation="MISEAGRAQSLAVEGPIAPFDARLTVPGDKSISHRAAMLGGVAE
                     GVTRIRNFLPANDCLATLDVMRGLGVEVEYRGGEVLVHGRGMEGLVEPARPLECGGSG
                     TTMRLMSGLLAGQPFYSVLAGNAQLSRRPMDRVARPLREMGATILGREGGRLAPLSIQ
                     GGNLHGISYKPPVASAQIKSAVLLAGLFARGETTVEEPTPTRDHTERMLMSMGADLAV
                     EGNAVRVRASHLKAIDVAVPGDISSAAFILAAAAIVPGSRVVVEGVGVNPGRTGFLHA
                     LERMGALVRLHGEHDSNGEPVADVEVSYAGLKGIAISAQDVPGMIDELPLLAVVATQA
                     EGVTEVRGAAELRVKETDRIATTAAELARMGARIEALPDGFRVHGPTPLLGARVSSHG
                     DHRLAMSLCIAALVARGVTTVEDTECADDSFPGFEAALRTLAEGGPGGE"
     gene            2852..3730
                     /gene="aroE"
                     /locus_tag="VFH60_08605"
     CDS             2852..3730
                     /gene="aroE"
                     /locus_tag="VFH60_08605"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET6313882.1"
                     /translation="MSSGAPGEAQDVEQAEQLVGLFGYPVEHSLSPAMQNAAFASAAL
                     PYRYVLLPATPGEFETEVGRCVSAGFAGWNVTVPHKERMVSLLDEASDEVLVIGACNT
                     VRVENGQLLGFNTDPAGFMRGLADAGGIQRGTRVVLLGAGGAARSVAWALSRKGYELL
                     ILSRDPEQSEQVAKSLTSRTHRNIESGSLHGVSLGDALKEAAMLVNCTPIGQWPHTNE
                     SPLPANVRLPEHLLVYDLVYRPRPTRLLRDAQIAGCRTQDGLAMLVNQGAASFRIWTG
                     QEAPLEVMWEAVRGEA"
BASE COUNT          743 a         1286 c         1556 g          720 t
ORIGIN      
        1 aagataaagc gggcgggagc gttcaaaccg cgtacctccc cctatagctt ccagggcatg
       61 ggcgaggaag ggctgcaaat cctggccgac gtgcgggagt cgctcggtat gtcgatcatc
      121 accgaggtga tggccccgga cgaggtggcc ctggtgagcc gctacgccga tatcttgcag
      181 ataggcacgc gcaacatgca gaactatcgc ctgctggaag aagtcggcag ttgcggcagg
      241 ccggtgctcc tgaagcgagg catgagctcc acgatagagg agctgcttct ggcagccgag
      301 tacatcctcg cgaacggcaa cagccaggtg atgctctgcg agcgcggtat ccgcaccttc
      361 gagcgcgcta cccgctccac cttcgacctg aacgccatcc cggtgctcaa gcagctctcc
      421 cacctgcctg tgatcgcgga cccaagccac ggcaccggcc ggtgggacct ggtgacgccg
      481 gtgtcgctgg gcgcggtagc ggcgggggcc gacggcctca tcatcgaggt gcacagccac
      541 cccgagctgg cgctctccga cggcgggcag tcgctacgcc ccgacaagtg ccgcgccctg
      601 ctggacgacc tggggcggat cgccaacgcc gtgggcaagg gcctcgacct ggcatctccc
      661 gtagtggcgg gggccgaggc ggtccatgcg gcttagcgag gccacggtcg gcatacacgg
      721 cctggggctg atgggcggct cgctcggact cgccctgggg ggcaagtgca agcggcgcat
      781 cggcatcgta cgcaggcccg aaacggcgga actggcgctc caaattgggg ctgtggaggc
      841 cgctaccctc gatttgcagg cgggtgccag tgccgccgat atagtagtgc tcgccacgcc
      901 tgcgcgccgg atcgtgatgg atgtggagga agcgggcagg cacatgcgcc ccggctcagt
      961 gctgatggac ctggggagca ccaagggggc cgtcgtggag gctatggacc gcctgccggg
     1021 gcacgtctcg gcggtaggcg ggcacccgat gtgtggcaag gaagtaggcg ggctggagca
     1081 cgccgaggct gcgctgttcc atgacaaggt gtttgtgcta accgccaccg cacgcacaga
     1141 tgaggccgct ttgaccctaa gccacgagcg tgtggaggcg gtaggggcgc acccggtgct
     1201 gatggagcct gggcagcacg accgggccgt agcctacgtg agccacctgc cttacctgat
     1261 ggctgccgcg ctggtgcgaa cggagaaaga ggccgccggg tgcgatccca cgttggaccg
     1321 gctggcggct agtggctttc gcgacacctc tcgcctggcc gcgagcgaag tgccgatgat
     1381 gcgcgatata ctgctgacca acaggcacgc gatagaggag gcgcttgcgt cgttcgaggg
     1441 gcagcttgac acggtgcgcc ggttgttgga cgatgaggca gcgttggagg ggtggatgct
     1501 ggacgcgcag agagagcgga gggcgatgtt cgtatgattt cagaggccgg acgcgcgcaa
     1561 tcgctggccg tggaggggcc tatcgcgccc ttcgatgcca ggctcaccgt gccgggcgac
     1621 aagtccatct cccaccgtgc cgccatgctg gggggcgtag ccgagggtgt cacccgcatc
     1681 cgcaatttcc tgcctgccaa cgattgcctc gcgacgctgg acgttatgcg ggggcttggc
     1741 gtggaggtgg agtatcgcgg cggcgaggtg ctggtgcatg ggcgcggcat ggaggggttg
     1801 gtcgagcctg cgcggccgct ggaatgcggc gggtcgggca ccaccatgcg gctgatgtcg
     1861 ggcctgctag cggggcagcc gttctacagc gtgctcgcgg gtaacgcgca gttgagccgc
     1921 aggcccatgg accgcgtagc ccggcccctg cgcgagatgg gcgcgactat actgggccgc
     1981 gagggtggcc gcctggcccc gctctccatc cagggcggca acctccacgg cattagctac
     2041 aagccgcccg ttgccagcgc gcagattaag tcggcagtgc tgctggcggg gctgttcgcg
     2101 cggggcgaga cgacggtgga ggaacccacg cccacccgcg accacaccga gcggatgctc
     2161 atgtcgatgg gtgccgacct ggctgtggaa ggtaacgccg tgcgcgtgcg agcgtcacac
     2221 ctcaaagcga tagacgtggc tgtgccgggc gatatctctt cggcggcttt catactggcg
     2281 gcagcggcga ttgtgcccgg ctctcgcgtg gtagtggagg gcgtgggggt gaaccccgga
     2341 cgtaccggct tcctgcacgc cctggagcgg atgggtgccc tggtccgtct gcatggcgag
     2401 cacgacagca acggtgagcc tgtggcggac gtggaagtgt cgtacgcagg cttgaagggc
     2461 atcgctattt cggcgcaaga cgtgcccggc atgatagacg agctacccct gctggcggtg
     2521 gtcgccacgc aggccgaggg cgtcacggag gtgcgcgggg ccgccgagct gcgcgtgaag
     2581 gagacggacc gcatcgctac cacggcagcc gagctagccc gcatgggtgc ccgtatcgag
     2641 gcgctgcccg acggcttccg ggtgcacggg ccgacccctt tgctgggcgc gcgagtaagc
     2701 agccacggcg accacaggct agccatgtcc ctctgtattg ccgccctggt ggcgaggggc
     2761 gtaacgacgg tagaggacac tgagtgcgcc gacgatagct tcccaggctt cgaagcagcg
     2821 ttgcggacgc tcgccgaggg aggtccgggt ggtgagtagc ggcgcgccgg gggaggcaca
     2881 ggacgtagag caggccgaac agcttgtggg gctgttcggc tacccggtgg agcacagcct
     2941 ctcgcccgcg atgcagaacg ccgctttcgc ctcggccgcc ctcccgtacc gctacgtgct
     3001 cctgcccgcc acgccggggg agttcgagac ggaagtcggc cggtgcgtgt cggcggggtt
     3061 cgcgggctgg aacgtcacgg tgccgcacaa agagcggatg gtgtcgctgc tggacgaggc
     3121 gagcgatgag gtgctggtga taggcgcgtg caacacggtg cgggtcgaga acgggcagct
     3181 actcggcttc aacaccgacc cggcgggctt catgcgcggg ctggccgatg ccgggggcat
     3241 acagcgtggc acgcgggtgg tgttgctggg ggcgggaggg gccgcgcggt cggtggcctg
     3301 ggcgctctcc aggaaggggt acgaactgct gattctctcc cgcgaccccg agcagtcgga
     3361 gcaggtagca aagtcgctga cttcgcgcac gcaccgcaat atagagtctg gctccctgca
     3421 cggggttagc ctgggtgacg cgctgaaaga agccgccatg ctggtcaatt gcacgccgat
     3481 agggcagtgg ccccacacca acgaaagccc cctgcccgcg aacgtgcgcc tgcccgaaca
     3541 cctgctggtg tacgacctgg tctacaggcc gcgccccacc cgcctcttgc gcgacgccca
     3601 aatagcgggc tgccgcaccc aggatggcct cgccatgctg gtcaaccagg gtgccgcctc
     3661 cttccgcatc tggaccgggc aggaggcacc gctggaggta atgtgggagg cggtgagggg
     3721 tgaagcgtga cgagtgacga gtgacgagtg aggggatgga cgctcgtggg tgtcactttg
     3781 ttcgggttgt gcggccaaaa ttccccttgt catcctgaga gggtgtctaa taatagggta
     3841 gagattgtaa aggaagagcg agggagaagg gtatgatgag agagaaggcc gctgaaatag
     3901 gttctatgca caccacgttt gctctctcct gccctccttc caacctatat gaccttgttt
     3961 ttgtgcctaa atcttcccct tacccttatt agacaccctc tgagcgtagc gaaggatcag
     4021 gtgggtaggc cagagtcttc aaccgacagc aggttggtgg cattaggaac acaggggcgt
     4081 gaataggaag agtcaagaac gggtggctga tccttcgcta cgctcaggtt gacaaggggg
     4141 actttggccc cgccaggccg acagagtgac ctcccggagg gtcgatttct ttagccgctt
     4201 cactcgtcac tcgtcactcg tcagcatcta tagtcgacaa gaaagtaggt aagcgaacat
     4261 gtcacacagg tttctaacgg gtggggagtc gcacgggcct tgcct
//
LOCUS       DASRWZ010000548         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_249838, whole genome shotgun sequence.
ACCESSION   DASRWZ010000548 DASRWZ010000000
VERSION     DASRWZ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249838"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..2240)
                     /locus_tag="VFH60_08610"
     CDS             complement(207..2240)
                     /locus_tag="VFH60_08610"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET6313883.1"
                     /translation="MLARLRADRVPNPRLIATLALFLGWLVMYLSTTSPTVNFIDSGE
                     LITALYEPGIAHPPGYPLYVLLGYVASHLLPGEVAWRVNAFSAFWGAMAVAVLFLLLL
                     EVAAFIRRSVVPLRTAPSRPNKRQKAAAGSVAPASGELKASPWPELVLAASAASLFAA
                     SSSFWSRTAQAKMYTLHYFFVVFLLLAALQYRAAYERDDASSARRWLLATTLGAGLSL
                     TNHMMTILLAPGVLVLLLAGSGWRARLNALLRRWYIVLPAVTLPLLLYLYLPWRASQS
                     PVMNWGAPTTWDDFYRHITGWQYRIYLLDTIREDLDQNFERVVNYLLQQWTIVTPVVL
                     LAGSGGAFMLARTAPVVFAATLLTALSTFLFAIAYGISEVEPYMVPVYIMIAVWLGGG
                     AVALHTWSATRDRQQTEEGHARRRQWLQAGAAAVAVLALVSIVIQYPRQNHRDDRLAE
                     QFVENVFSTLPQNSILITDYWDFYAPTYYMQQVRGLRPDIAIVDKSLLRYPWYLEQLA
                     RRHPFLVENSSDVAEQFRAEQRKWVDEEPFDPALLDHLYMSLLTSFVERNSGTHSAFL
                     LMLPPCDAVPAPSRCDDNQIAPGYLRQIYGLATRLLRAGDPPAALPAEPTYAVRGIVG
                     SQVPMDEFARINSEAYARAYRRLGQLYTAANDTERASRMLERAEQIEQALGIR"
     gene            2345..3025
                     /locus_tag="VFH60_08615"
     CDS             2345..3025
                     /locus_tag="VFH60_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313884.1"
                     /translation="MSQSRSSSGFSGRGFLAGGLLVPVALLCTLLLAACNLTVNDGSP
                     RRSLPTPTSLSTIEPGLTGDEEEPELDVTAAPGTVVSTRPPRPTRTPVPTSTPTPPPT
                     STPTATHTPGTPTPTLTPTSTLTPFATPTPFATFTPIPPIPTVTPRPFVSPTPRPTGT
                     ATSVPTATPVPTLAPLPPTETPTPEIIPLPEATDTPVPVDTPTPEPTPVPTDTPFPLG
                     ELITPPVP"
BASE COUNT          574 a         1019 c          984 g          530 t
ORIGIN      
        1 tcggcatggc cccctttggt aaccttcagg cgggcctcat cgcggaccac ttcggcgcac
       61 cgctggccct gttcgtcggg gcggtgacgt gcctgctcgt aacagtgctg gtcctcttga
      121 agaacaggca gatactgtca ctcctgtagc ccggcgaaca ggtaatagaa cgaggggcct
      181 tcactcgtaa aggaaggccc tttcgtttac cttatcccca gcgcctgctc gatctgctcg
      241 gcccgctcca acatgcggct ggctcgctcc gtgtcgttgg ccgccgtgta aagttggccc
      301 aggcggcggt aagcgcgggc gtaggcctcc gagttgatgc gggcaaactc gtccatcggc
      361 acctgactgc ccacgatgcc ccgtacggca taagttggct cggcggggag ggccgctggt
      421 gggtccccgg cccgcaacag gcgcgtagcc agtccgtaga tttgacgcaa gtagccgggg
      481 gctatctggt tgtcgtcgca gcggctggga gcgggcacag cgtcgcaggg gggcagcatc
      541 agcaggaacg cggagtgggt gccggagttg cgctccacga acgaggtaag gagcgacatg
      601 taaaggtggt caagcagcgc ggggtcgaat ggctcctcat ccacccactt gcgttgctcc
      661 gcgcggaact gctccgccac atcgctggag ttctcaacga ggaaagggtg ccgccgggcg
      721 agctgttcca ggtaccaggg gtagcgcagc agcgacttgt ctacgatcgc gatgtcgggg
      781 cgcagccctc gcacctgctg catgtaataa gtcggcgcgt agaagtccca gtaatcggta
      841 atcaagatac tgttttgtgg tagcgtggaa aagacgttct ccacgaactg ctcggctagc
      901 ctgtcgtccc ggtggttctg gcgcgggtac tgtatgacga tgctcaccaa cgccagcacg
      961 gccaccgctg ccgcgcctgc ctgcaaccac tgcctgcgac gtgcgtgtcc ttcttccgtt
     1021 tgctgcctgt cccgcgtcgc cgaccaggtg tgtagcgcca cggccccgcc gcccagccag
     1081 accgcgatca tgatgtacac gggcaccatg taaggctcta cctctgagat gccgtacgct
     1141 atcgcaaaga ggaaggtcga aagggcagta aggagggtgg cggcgaacac cacgggcgcg
     1201 gtgcgggcca gcatgaacgc cccgccactt ccggccagca gcacaacagg tgtaactatc
     1261 gtccactgtt gcaacaggta gttcactacg cgctcgaagt tttggtccaa gtcttctctt
     1321 atcgtgtcta acaagtatat gcggtactgc cagcccgtga tatgccggta gaagtcgtcc
     1381 caggtggtgg gcgcgcccca gttcatgaca ggagactgcg aggctcgcca cggcaggtag
     1441 aggtaaagca gcaagggcaa cgtcaccgcc ggaagcacga tgtaccagcg acgcaggagg
     1501 gcgttcaggc gcgcacgcca accgctgccc gcaagtaaca acaccagtac accgggagcg
     1561 agcagtatgg tcatcatgtg gttggtcagc gatagccccg ctcccagggt ggtcgccagc
     1621 agccaccgcc gggcgctgct ggcgtcatct cgctcgtatg cggcgcggta ttgcagggcc
     1681 gccagcagca ggaacaccac gaagaagtag tggagagtgt acatcttggc ctgcgccgtg
     1741 cggctccaga agctgctcga ggcggcaaac aacgaggcgg cggaggctgc cagcaccagt
     1801 tcgggccacg gcgacgcctt cagttcgcca gaggccggcg ctacggaccc ggcagccgcc
     1861 ttctgcctct tgttcgggcg ggagggcgcc gtccgcaggg gcacgaccga ccgccggatg
     1921 aaagcggcca cttcgagcag gaggaggaat agcaccgcca cggccatcgc gccccagaag
     1981 gccgagaagg cgttgacacg ccaggccacc tcgccgggca gcaggtgaga tgctacatat
     2041 cccagaagaa cgtagagtgg gtagccgggc gggtgcgcga taccaggctc gtacagggcg
     2101 gtgattagct ccccgctgtc tatgaagttt acggtgggcg aggttgtgga caggtacatc
     2161 accagccagc ccaggaacag ggcgagggtg gctatgagcc tggggttggg gacgcggtcg
     2221 gctctgaggc gggccagcat ggggtctcct gtcgttgtgt ggctccaaat atgttaccac
     2281 gtgcgggcac gcgctaccac ccgcagaatt gaagcctccc tgtgcatgtg ttatagtcct
     2341 gtatatgtcg cagtcacgtt cttctagcgg cttcagcgga aggggctttc ttgccggggg
     2401 cctgctggtg ccggtcgccc ttctttgcac gctcttgctt gccgcgtgca acctgacggt
     2461 gaacgacggc tccccgcgcc gcagcctccc cacgcccacc tccctgagca ccatcgaacc
     2521 gggactgaca ggtgatgagg aggagcccga gttggacgtt acggcggctc cgggcacggt
     2581 agttagcacc agaccgccca ggccgacccg cacgcccgtg cccacttcca cgcctacacc
     2641 cccgcccacc agcacgccca ctgcgaccca cacgccgggc acgcccacgc ccacgctcac
     2701 gccgacgagc acgctgacgc ctttcgccac gccgacgccg ttcgccactt ttacgcccat
     2761 acccccaatc ccgacggtga cgccgcgccc gttcgtctca ccaacgcctc gccccactgg
     2821 cacagcgacc agcgtgccca cggccactcc ggtgccgacg ctcgcgccgc tcccgccgac
     2881 tgagacgccg acccccgaaa tcataccgct gccggaggca actgataccc cggtgcccgt
     2941 ggacactcct acgcctgaac ccaccccggt gccgaccgat acgcccttcc cactgggtga
     3001 gctaatcacc ccacccgtgc cgtgaggcgc aaagaccatc aaactgctgt gaggtaatgc
     3061 cgtatgagca cgaaggttgg tgtcattggt ctcggggcga tgggcct
//
LOCUS       DASRWZ010000549         1947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1072651, whole genome shotgun sequence.
ACCESSION   DASRWZ010000549 DASRWZ010000000
VERSION     DASRWZ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072651"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1837
                     /locus_tag="VFH60_08620"
     CDS             <1..1837
                     /locus_tag="VFH60_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET6313885.1"
                     /translation="VSEETNLNAALEEHGIEPVETDLGEWIVQLAHERPSHMVMPAIH
                     KSRQQVGQLFTDTTGYEVSREDIAEQVGVARRELRRDFLEAGLGINGANALIAESGTV
                     MFIENEGNARLVTSLPRVHVVFAGIEKLVPDYPAAMLQLRLLARSATAQAITSYTSFV
                     SGPPEPGKEMHVVLLDNGRMQMREHPLIKEALRCIRCAACANVCPPYAVVGGHVFGYI
                     YSGAIGLVNTPYHHGLEAGAGPQSLCVSCNACATVCPVGIPLPQQILSVRSQVVEKKS
                     YPFPVRFALSLWSQPRLFDIALRVSARMSRPFSDNGFLRVKRTGANLVPKVRALTSWR
                     TPPAPAPVPARDTLAARSTRTQTVPSKAQGLRVAYFIQCITDRLFPGMAEAVVSVLEA
                     CGVEVTVPQGQHCCGLPNLDAGDLPRARRMAKQTIEALESVRADYILTGGASCAIAML
                     HEYEGLFEGEPAWQIRALALKEKVIDFVTFIDRVAQLEQGTLGKPGANLGPVTYHSFC
                     QSTNILGIVDAPKRIIRDVLGLELRDLPEGTVCCGFGGSTSIGHPEVASQILKRKLDN
                     LASTGAKVLVTDNPGCIMHLRGGIDAARMDVKVMHLAELMAAHLG"
BASE COUNT          363 a          661 c          614 g          309 t
ORIGIN      
        1 ggtgtcggag gaaaccaacc tgaatgccgc gctggaggag cacggcatag agcctgtcga
       61 gaccgacctg ggcgagtgga tagtgcagct cgcgcacgag cggccctccc acatggtcat
      121 gcccgccatc cacaagagcc gccagcaggt gggccagcta ttcaccgaca ccacgggcta
      181 cgaggtaagc cgggaggaca tagccgagca ggtgggcgtg gcccggcgtg agttgcggcg
      241 cgacttcctg gaagccgggc tgggcatcaa cggggccaac gccctcattg cggagagcgg
      301 cacggtcatg ttcatcgaga acgagggcaa cgcccgcctg gtgacttccc tgccccgcgt
      361 acacgtcgtg ttcgccggta tcgagaagct ggtgcccgac taccccgccg ccatgctgca
      421 actgcgtctc ctcgcccgct cggccaccgc gcaggcgatc acctcctata ccagcttcgt
      481 gtcggggccg ccggaaccgg gcaaggagat gcacgtcgtc ctgctcgaca acgggcgcat
      541 gcagatgcgc gagcacccgc tcatcaagga ggccctgcgg tgcatacggt gcgccgcctg
      601 cgccaacgtc tgccctccct acgcggtggt gggcggccac gtcttcggct acatctactc
      661 cggggctatc ggcctggtga acacgcccta ccaccacggg ctggaggcgg gcgcggggcc
      721 gcaaagcctg tgtgtttcgt gcaacgcctg cgccacagtc tgccccgtgg gcatacccct
      781 gccccagcaa atactctccg tgcgctcgca ggtagtggag aagaagtctt atcccttccc
      841 ggtgcggttc gccctgtcgc tatggtcgca gccgcgcctg ttcgatatcg ccttgcgggt
      901 gtcggcccgg atgtcgcggc ccttcagcga caacggcttc ctccgtgtga agcgcaccgg
      961 ggccaacctc gtgccgaagg tgcgggcgct cacctcctgg cgcacgcccc ccgcgcccgc
     1021 ccccgtgccg gcccgtgaca cactggccgc ccgcagcaca cgcacgcaaa cggtgcccag
     1081 caaggcgcaa gggctgcgag tggcctactt catccagtgc atcaccgacc gcctctttcc
     1141 gggcatggcc gaggccgtag tctccgtgct cgaagcctgc ggcgtagagg tgaccgtgcc
     1201 gcaggggcag cactgttgcg gcctgccaaa tctggacgcg ggcgacctgc cacgggcgcg
     1261 caggatggcg aagcaaacga tagaggcgct cgaaagcgtg cgcgccgact acatccttac
     1321 gggcggtgca agctgcgcca tagccatgct ccacgaatac gagggcctgt tcgaggggga
     1381 gcctgcctgg cagatacgcg ccctcgcgct caaagagaaa gtgatagatt tcgtcacctt
     1441 catcgaccgg gtagcgcaac tggagcaggg gacgttgggc aagcccggtg caaacctcgg
     1501 ccccgtcacc taccacagct tctgccaatc gaccaacatc ctgggcatag tggacgctcc
     1561 gaagcgcatc atacgcgacg tgctgggtct ggaactgcgc gacctgccgg aaggcaccgt
     1621 ctgctgcggc ttcggcggct cgacctccat aggccacccc gaagtggcct cccagatact
     1681 caagcgcaag ctggacaacc tcgcctccac cggcgcaaag gtgctcgtca ccgacaaccc
     1741 cggctgcatc atgcacctgc gcggcggcat cgacgcagcc cgcatggacg taaaggtcat
     1801 gcacctcgcc gaactcatgg cggcacacct ggggtgagag tcaaagagtg acgatgaaac
     1861 cgcaaaggcg caaagagcgc aaagtttaag gattgtttag cgggtacgaa caggacaagg
     1921 gtactaatcg tgtacccttg tcctttt
//
LOCUS       DASRWZ010000550         4328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_249966, whole genome shotgun sequence.
ACCESSION   DASRWZ010000550 DASRWZ010000000
VERSION     DASRWZ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249966"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..640
                     /locus_tag="VFH60_08625"
     CDS             218..640
                     /locus_tag="VFH60_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313886.1"
                     /translation="MTEKDKIKKARQKSKFAAMEALRDNKGLPIPPRKAMSRAKKASK
                     KLAYPLPPYAGANPVQLYRWGQFEWRKLGRWQRVVLPVVVLNALLTWERERELQDRYW
                     DLVDRAQAKETAAHAYGAYGPRPRKRKLLGIIPLPGRK"
     gene            787..1830
                     /gene="ruvB"
                     /locus_tag="VFH60_08630"
     CDS             787..1830
                     /gene="ruvB"
                     /locus_tag="VFH60_08630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET6313887.1"
                     /translation="MVERLISAKTRENEEEIEQTLRPRRIAEYIGQEKLKDHVEVMLQ
                     AAKGRGEVLDHLLFYGPPGLGKTTISQVIANEMGVPIKTTSGPAIERPGDLASVLTNL
                     KANEILFIDEIHRLNRVVEEVLYPAMEDFALDVMIGKGPSARSLRLSLPPFTIIGATT
                     RLAMLSSPLRDRFGVTFRLEYYSREAMEMIITRSARILGVACEEDGAKVVASRSRGTP
                     RIANRLLRRVRDYAQVRADGVITAPVAQEALRRLEIDSLGCDETDRRILHAIIDKFDG
                     GPVGLDTIAAAISEESDTIMDVYEPYLLQLGFLARTPRGRIATRRGYEHMGVPYPLRM
                     GMEEIDVEQGKLF"
     gene            complement(1892..2341)
                     /locus_tag="VFH60_08635"
     CDS             complement(1892..2341)
                     /locus_tag="VFH60_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313888.1"
                     /translation="MADRTLRPTSNALRAAGLLPQNQPAHHVAPGDDVPPQPASVSQI
                     FGPVRQVGDKTLIDVRSVRYVSGLLARFLKHGNEAKLARQANSVWIGSGGSYVLQNLV
                     AVVEVSELGVRIRAVRWPWLLILAGAFAIAWNVYWALRTVREWRERP"
     gene            complement(2334..2621)
                     /locus_tag="VFH60_08640"
     CDS             complement(2334..2621)
                     /locus_tag="VFH60_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313889.1"
                     /translation="MSKRVLRGTPIYATRRGERIALVPEVRLSRMRLLRRSSGQGGVT
                     LKPAALYVIDRSGVKRVAVEPPTARGLGLLALSCLLAPALNWLSRRGSTHG"
     gene            3087..3890
                     /locus_tag="VFH60_08645"
     CDS             3087..3890
                     /locus_tag="VFH60_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HET6313890.1"
                     /translation="MAGELAGKFSGLLLDMDGVLYRGNDALEGATELFPALREAGLSF
                     ILLTNNATLRAADFSDKLARMGIDVEAGSILTSAGATATYLRTHFPEGGGVYVLGEEA
                     LVRTIEEVPNFHVEAERPEFVVAGLFFGFDYQALRTACTAIRRGARFIATNVDTTLPV
                     EGGDFWPGAGSLVAAVQACSGVAPTVIGKPSVHGGEMALEKMGLPANQVLCVGDRLDT
                     DIVMGRDAGMPTALLLTGVSQPADIERTGIAPDYIFDDLPALMSALGIG"
     gene            3923..4312
                     /locus_tag="VFH60_08650"
     CDS             3923..4312
                     /locus_tag="VFH60_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET6313891.1"
                     /translation="MRDVVRTEGAPGAIGPYSQGIKVGGLVFTAGQVGFDPATGQFVE
                     GGVEEQTRRTLENISAILEAAGTSLDRAVKVTVFLSDMSLFEAMNGVYKQFFSDTPPA
                     RTTVAVAGLPRNALVEIECVASLDDGQ"
BASE COUNT          862 a         1317 c         1370 g          779 t
ORIGIN      
        1 atcaccggcc tcggctacga gagggtgcag ttcagacaat caccctagca agaagtgccg
       61 agtgccgagt gccgagtgaa gagacgaaaa cgcaaagacg caaaggacgc aaagaactcg
      121 caaagagtag aaatgtttag gatgggtggt actcggtact cggtactcgg tactcggcac
      181 tcggcactca tcacgtaatt cccgaacgga ggaaggcatg acagagaagg ataagatcaa
      241 gaaggcgcgg cagaagtcga agtttgccgc gatggaagcg ttgcgggaca acaagggctt
      301 gcctatcccg cctcgtaagg ctatgtcgcg ggccaagaag gcctcgaaga agctggctta
      361 cccgttgccg ccgtacgcgg gtgccaaccc cgtgcagctt tatcgctggg ggcagttcga
      421 atggcggaag ctggggcgct ggcagcgggt ggtgttgcct gtggtcgtgc tgaacgccct
      481 gcttacatgg gagcgcgaaa gggagctaca ggaccgctac tgggacctgg tggaccgcgc
      541 ccaggccaag gagaccgcag cccacgctta cggcgcttac gggccccgcc ccaggaagcg
      601 caaactactc ggtatcatac cattgccggg cagaaaataa gtcttcgtca cgggtatttg
      661 ggtgtctctt gcacgtcgcc gtaaatcggt tgcacctggc gcggaaaagt tgatgaatct
      721 gctcggtttt gtggtataat gtagcaaata tgttctattg ttgtgcgctt gagtggagat
      781 tttgagatgg tagagcgact gatttcggcc aaaacgcgag agaacgaaga agagattgag
      841 cagacgctcc ggccccgccg catagccgag tacatcggcc aggagaagct gaaggaccac
      901 gtcgaggtca tgcttcaggc ggccaagggg cgcggggagg tactggacca cctgctcttt
      961 tacggccctc ccggcctcgg caagaccacc atcagccagg tgatagccaa cgagatgggt
     1021 gtgcccatca agaccacctc cggccccgct atcgagcgcc ccggcgacct ggcgtccgtc
     1081 cttaccaatc tgaaggccaa cgaaatcctg ttcatcgacg agatacaccg cctcaaccgc
     1141 gtggtggagg aggtgctcta cccggcgatg gaggacttcg cgctcgacgt gatgataggc
     1201 aaggggccga gcgcccgctc cctgcggctg tcgctgcccc ccttcaccat catcggggcc
     1261 accacgcgcc ttgcgatgct ctcctcgccc ctgcgcgacc gttttggtgt gaccttccgc
     1321 ctcgaatact actcgcgtga ggcgatggag atgattatca cccgctcggc ccgcatcctg
     1381 ggcgtggctt gcgaggagga cggagcgaaa gtagtcgcct cccgcagccg tggcaccccg
     1441 cgtatcgcca accgcctact gcgccgcgtg cgtgactacg cccaggtgcg cgccgacggt
     1501 gtcatcaccg cccccgtggc ccaggaagct ttgcgccgtc tggagatcga cagcctcggc
     1561 tgcgacgaga ccgaccgccg catcctgcac gccatcatcg acaagttcga cggcggcccg
     1621 gtcggcctcg acaccatcgc ggcggccatc agcgaggagt cggacacgat catggacgtg
     1681 tacgagccgt acctgctgca actcggcttc ctggcacgca cgccgagggg ccgcatcgcc
     1741 acccgcaggg ggtacgagca catgggcgtg ccctatccgc tacgcatggg catggaggaa
     1801 atcgacgtgg agcaaggcaa gctcttctaa gcccacccca caataaagca ggagacccgt
     1861 ctgcactgga cgggtctttt ttattcggct actatggcct ctcccgccat tcgcgcaccg
     1921 tccgcaacgc ccagtagacg ttccaagcaa tagcaaatgc gcccgccaat atcagcaacc
     1981 agggccaacg cacggcgcgg atgcgaacgc ccagctcgct cacctcgact acagccacca
     2041 ggttttgcag gacgtagctg ccaccgctac caatccatac agagttggct tgccgggcca
     2101 acttcgcttc attaccgtgc ttcaggaagc gtgcgagaag gccgctgacg tagcggaccg
     2161 acctgacatc aatgagggtc ttatcaccca cctgtcgcac cgggccaaag atttgcgaca
     2221 cagatgcggg ttggggcggt acgtcgtcac ccggcgcaac atggtgcgcg ggctggtttt
     2281 ggggcaggag gcccgcggcg cgcagggcgt tcgatgtggg tctgagggtc cggtcagcca
     2341 tgcgtgctac ccctgcgtga gagccagttc agcgctgggg ccagcaggca agatagtgca
     2401 agcagcccca gcccgcgcgc ggttggaggc tcaaccgcta cccttttcac gccgctccgg
     2461 tcgatgacgt acagggcagc aggcttaagt gttaccccgc cctggccgga agagcgccgc
     2521 aacaaccgca ttctcgaaag ccgcacttcg ggcacgaggg ctattctctc gccccggcgc
     2581 gtcgcgtaga tcggcgtacc tcgtagaacc cgtttggaca tgaaatctca cctctggtat
     2641 gcatcggtcc aggcaccccg ccgggttgga tgctcggacc ctagtccttg accctcatac
     2701 tggcagtgtg atgctgtggt gagcatcatg cggagggcag tacagtatac gacagtcggt
     2761 gaggaaggca gtatagaggg aagagggctg tgtcagagta gatcagcagg gccaaacatc
     2821 cccctgtcat cctgagcgta gcgaaggatc taagatgttg cacttactac taggttggac
     2881 cttcactacc agagtccctg actttcaaca gcatccctcg ccaccttctt tagatccttc
     2941 actacgttca ggatgacagg ggggaatgtt tggtcctgct actggtacat caccttcttt
     3001 gccccacccg acagggaaag cgctcgcttg ttgcggcccc tcgatttgcc ccataatggt
     3061 ccggttgtgt agcgaagggg aggcttatgg caggcgagct agcaggtaag ttttcggggc
     3121 tactcctcga catggacggc gtgctctacc ggggcaacga cgccctggaa ggcgcgaccg
     3181 agctgttccc ggctttgcgc gaggcggggc tttcctttat acttctcacc aacaatgcca
     3241 cgctcagggc ggccgatttt tcggacaaac tggcgcggat gggcatcgac gtggaggccg
     3301 gtagcatcct cacctccgcc ggggccacgg ccacgtacct gcgcacgcac ttcccggaag
     3361 gtggcggcgt gtacgtgctc ggcgaggagg cgctggtccg cacgatagag gaggtgccca
     3421 acttccacgt cgaggccgag cggcccgagt tcgtggtggc ggggcttttc ttcggcttcg
     3481 actaccaggc gctacgcaca gcgtgcaccg ctatcaggcg aggggcgcgc ttcatcgcca
     3541 ccaacgtcga tacgaccctc ccggtggaag gcggcgactt ctggcccggc gcgggcagcc
     3601 tcgtcgcggc agtgcaggct tgctccgggg tagcgcccac cgtcataggc aagccaagcg
     3661 tgcacggcgg cgagatggcc ctggagaaga tggggcttcc ggccaatcag gtgctctgcg
     3721 ttggcgaccg actcgacacc gacatagtga tggggcgcga cgccgggatg cccaccgccc
     3781 tcctgctcac cggggtatcg cagcccgctg acatcgagcg caccgggatt gcgccggact
     3841 atatatttga cgacctgccc gcgctgatgt cggcgctcgg catcgggtaa gcatcagaac
     3901 ggcaaaggga gaagcagtta acatgagaga cgtagtcagg acggaggggg caccgggagc
     3961 gattggcccc tactcgcaag gtatcaaggt tggcgggctg gtgttcaccg ccgggcaggt
     4021 tggcttcgac cccgccacgg gccagttcgt ggagggtggc gtggaggagc agacccggcg
     4081 caccctggaa aacatcagcg ccatactgga ggcggcgggc acgtccctcg atcgtgcggt
     4141 caaggtcact gtgttcctga gcgatatgtc gcttttcgag gccatgaacg gcgtttacaa
     4201 gcagttcttc agcgatacgc cccccgcacg tactacagta gcggtggcgg gcctgccgcg
     4261 caacgccctg gtggagatag aatgcgtggc ctccctcgat gacgggcagt agacacgagg
     4321 tatgagga
//
LOCUS       DASRWZ010000551         4086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_798738, whole genome shotgun sequence.
ACCESSION   DASRWZ010000551 DASRWZ010000000
VERSION     DASRWZ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798738"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1893)
                     /locus_tag="VFH60_08655"
     CDS             complement(202..1893)
                     /locus_tag="VFH60_08655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6313892.1"
                     /translation="MAKKKKNQSIAPVPLTRGQLSRAQQEQRKIRNLYATAVAVGTVV
                     VLVIALAAISTYIIRPNQVIGSVTYVEQRNITRASYDKARRYNLWQLMQQNALQRQFS
                     QSTTALQDTQNEQLQFRNVSNEQTLDEQTVVGLVNAEVLRQGAKKDYQIDPNNDALKT
                     YVLKQFEPQPTAPVTPSPSAAAASPTLAITATQTTTQTTTQTRTPSPTRTATPGSPTA
                     TPTATATFPPVPGAEQTAVTSYNQYVTALDMSVDPNSNNGFCQFGCPDISEDDYLNLI
                     VKPNYLREKVLEVQATEIMTQVEQIHAQHILTTTKEGAEALRQRLLRGEDFTMLANTE
                     SKEQLDAIAQGQTPTGGDLGWFPREDSNLIQTFVEGAWPVPAGQISEPVETTFGWHII
                     KVLERDPKRALSADVVEQKKTKKYDDWFAGVVAKSQISPQPTPTPPPPTAAVSEPTIP
                     PVVASPQVTTTTTLTGTQTVPATAPAGGTPTTGAATTPGAGTPAGGTATTGTTATAGA
                     ATPTTATTGTASTPAATGTTSGSAANTPTVPATAAAGTTSTVGATRTATGATPTP"
     gene            complement(2067..2579)
                     /gene="rpsR"
                     /locus_tag="VFH60_08660"
     CDS             complement(2067..2579)
                     /gene="rpsR"
                     /locus_tag="VFH60_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET6313893.1"
                     /translation="MADQTDNPNTNGNGGQQAPQAAAPGGPGGPGAPGGGQDRPAGPG
                     GPGGPGGPGGGGGGGGYRSGPRPGGGGGGGFRSGPRPGGAGGPGGRPRGRGDRYVPRR
                     KVCSFCVDRVIEIDYKDVARLRRYLSERSKIEPRRKTGTCARHQRGLSVAIKRARHMA
                     LLPYVGPHAR"
     gene            complement(2713..3225)
                     /locus_tag="VFH60_08665"
     CDS             complement(2713..3225)
                     /locus_tag="VFH60_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET6313894.1"
                     /translation="MSGGLNKVMIIGNLGKDPEMKYTQQGTPITSFSVAVSRTWKGPD
                     GSSQEETEWFRVVAWERLAEQCNEFLRKGRKVFIEGRLKTREWTGQDGQKRFTTEVVA
                     TSMILLDPKQGGDYSGGGGGGYREERQAPARQGAGSGAAAGGAGGGAAGGFGHSYDDD
                     ADLDVDDIPF"
     gene            complement(3275..3658)
                     /gene="rpsF"
                     /locus_tag="VFH60_08670"
     CDS             complement(3275..3658)
                     /gene="rpsF"
                     /locus_tag="VFH60_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET6313895.1"
                     /translation="MRDYELMYIVRPTVGDEEFPAATERVDTIIANIGGEVADKQPWG
                     KRRLAYPIDKHEDGYYVLARIKLEPGRTGALEEQLRISEDVIRHVLVEPVAPHVPRPP
                     RERPAGQDGEMPRNDMPRGEMPRRY"
BASE COUNT          645 a         1232 c         1343 g          866 t
ORIGIN      
        1 cgactgccac ctgatccttc gctacgctca ggatgacaag ggggtagatg gtcctgtctt
       61 ggttactttc ttgcaagctg ctccactcag gatgacaaag gagaagagag gctgacgagg
      121 gagaggcctg gctatcgcgg gcgcgaagaa gggaccgaat ttaccgtccg gtccctccac
      181 ctgttcagtg tattgcatgt cctaaggcgt aggagtggcc cccgttgccg tgcgggtagc
      241 tcctaccgtg ctggtagtac cggccgctgc tgtagcgggt actgtgggcg tatttgctgc
      301 gctgccggag gtcgtgcccg tcgcggcagg cgtgctggcc gtgcctgtgg tggctgtggt
      361 gggagtagct gcaccggccg ttgcagtggt gcccgtggtg gccgtaccgc cggcaggtgt
      421 gcccgcgccg ggtgtggtcg cagcacccgt ggtgggagta ccaccggccg gagccgtcgc
      481 cgggaccgtc tgcgtgcccg tcagcgtggt agtggtggtg acctgcggac tggccacgac
      541 cggcggtatg gtcggttcgc tcaccgcagc ggtgggcggc gggggcgtgg gtgtcggctg
      601 cggcgagatt tgggactttg ccacgacccc ggcgaaccaa tcgtcgtact tcttggtctt
      661 cttctgctcg accacgtcag ccgatagcgc ccgcttgggg tcgcgttcca gcaccttgat
      721 gatgtgccag ccgaaggtcg tctccacagg ctccgagatc tgcccggcgg gcaccggcca
      781 cgcaccttcc acgaacgtct gtatgaggtt gctgtcttcc cttgggaacc agccgaggtc
      841 gccgccggtg ggcgtctggc cctgcgcgat ggcgtctagc tgctccttgg actcggtgtt
      901 ggcgagcatg gtgaaatcct cacccctgag cagccgctgg cgcagcgcct cggccccttc
      961 cttggtggtc gtaaggatgt gctgggcgtg aatctgctcc acctgcgtca tgatttcggt
     1021 cgcctgtacc tcaagcacct tttcgcgcag gtagttgggc ttcactatga ggttgaggta
     1081 gtcgtcctcc gagatgtcgg ggcacccaaa ctggcagaag ccgttgttcg agttaggatc
     1141 gacgctcata tccagcgccg tcacgtactg gttgtacgaa gttacagccg tctgctcggc
     1201 acccggcacc ggcgggaagg tagcggtcgc cgtaggggtg gccgtgggcg agccgggggt
     1261 ggccgtacga gtgggagagg gagtgcgcgt ctgcgtggtg gtctgcgtgg tggtctgcgt
     1321 agcggtgatg gccagggtgg gcgatgcagc ggccgcgcta ggtgaggggg tgacgggcgc
     1381 tgtcggctgc ggctcgaact gcttcaggac gtaggtcttc agagcgtcgt tgttcggatc
     1441 gatctggtag tccttcttag cgccctggcg cagcacctcg gcgttgacca ggcccacgac
     1501 cgtctgctcg tccagcgtct gctcgttgct cacgttgcgg aactgaagct gttcgttctg
     1561 ggtgtcctgg agggcggtag tgctctgcga gaactgtcgt tgaagcgcgt tctgctgcat
     1621 cagctgccaa aggttatacc ggcgcgcctt gtcatacgag gcgcgggtga tgttgcgttg
     1681 ctccacgtag gtgacgctgc cgatgacctg gttcggcctg atgatgtaag tactgatcgc
     1741 ggcaagcgcg atcacaagca ccaccactgt acctaccgcc actgccgttg cgtacaggtt
     1801 gcgaatcttg cgctgctcct gttgagcgcg cgaaagctgc ccgcgcgtca agggtaccgg
     1861 cgctatagac tggttcttct ttttctttgc catagtgggg gttctgaggc gtgggagtga
     1921 ctcataacgc gtaacacgta acgcgtaact gcgttgcaaa ccaaccaggc ggctcggagg
     1981 gagcagagtt cccaagttgg taaaccgtct aagtgttacg tgttacgtgt tacgtgtcac
     2041 tcctacgcct cagctttcat ctccttctag cgagcgtggg gacctacgta gggcaagagg
     2101 gccatgtggc gggcgcgctt tattgcgacg ctgaggccgc gctggtgtcg ggcgcaggtg
     2161 ccggtcttgc ggcgtggctc gatcttgctg cgctcggaga gatagcggcg caggcgcgcc
     2221 acgtccttgt agtcgatctc gatcacgcga tcgacgcaga aggagcacac cttgcggcgg
     2281 ggaacgtagc ggtcgccacg cccgcgcgga cggccaccgg ggccacctgc gccaccgggg
     2341 cgcgggccac tgcggaagcc acctccgcct ccaccgccgg gacgagggcc gctacggtag
     2401 ccgcctccgc cacctccacc gccgggacca ccggggccac cagggccgcc ggggcctgct
     2461 ggacggtctt gtccgccgcc tggagcaccg ggtccaccgg gtccaccggg agcagccgcc
     2521 tgaggggcct gctgtccgcc gtttccgttt gtgttagggt tgtcagtctg gtctgccata
     2581 ttcctgtgta acttcctttt tgactacgta ccaggggcgc ggcttacaca tggcgctcgt
     2641 cgtgcgagca ccggcgaccg tttgcaccac ccggtacact gtttagttcg gtctcagagg
     2701 actaagctgg tcttagaagg ggatgtcgtc cacgtcgagg tcggcgtcat cgtcgtagct
     2761 gtggccgaag ccaccagccg cgccgccacc ggctccaccc gcggccgcac cgctaccggc
     2821 accctggcgg gcgggggcct gccgctcctc gcgatagccg ccgccaccac cgcccgagta
     2881 gtcaccgccc tgcttggggt caagcagaat catggaagtg gctaccacct cggtcgtgaa
     2941 gcgtttctgg ccgtcctggc cggtccactc gcgcgtcttg aggcggccct cgatgaacac
     3001 tttcctgcct tttctcagga actcgttgca ctgctcagca agcctctccc aagccaccac
     3061 gcggaaccat tcggtctcct cctgtgacga gccgtcgggg cctttccagg tccggctgac
     3121 cgccacggag aacgaggtga taggggtacc ctgctgggtg tacttcatct ccgggtcttt
     3181 gcccaggttg ccgatgatca ttactttgtt caagccacct gacatctacg gcctccatcc
     3241 actcaaaaat cagctaataa ctcgtaaatc aagcctagta tctgcggggc atttcgccac
     3301 ggggcatgtc gttgcggggc atttcgccgt cctgcccggc cggccgctca cgtggcgggc
     3361 ggggtacgtg gggggccacc ggctccacga gcacatggcg aatgacgtcc tccgatatgc
     3421 ggagttgctc ttcgagagcg ccggtccgcc cgggctcgag ctttatccgc gccaggacgt
     3481 agtagccatc ctcgtgcttg tcgatggggt aggccaggcg gcgcttgccc cagggctgct
     3541 tgtcggccac ttcaccgccg atgttggcga tgatggtatc cacgcgttcg gtggcggcgg
     3601 ggaactcctc atcgcctacg gtgggccgga ctatgtacat cagttcgtag tcgcgcatct
     3661 attgtcacac ctcctctcta tgggcttgcc ccggcgcctt gattctggag gggtgtgaag
     3721 tctccagggt cgttcggagc agaaaggtaa gggatattac ggcagtgcgg cacatcgcca
     3781 ttgtgtgaaa atgcaaactt tccgcactgt ctggcattat accacatgcg cctgctctta
     3841 gcaaaagcgc gtcaaaatgg gagcgttcag ataggagatg taatacagtc ctatcgttct
     3901 gaggtgtggc aagacctgaa cctgagtagc tacactatgc gcaggccatc tcaatgccaa
     3961 ttggaggcta aggttcagcc ttatagttag tgtgggcctc agacgggact aggcccgtat
     4021 cagccccaca gccgggacag tatctccttt cccgccaaac ggaagcaggg gcgatgctcc
     4081 aacact
//
LOCUS       DASRWZ010000552         3520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_44579,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000552 DASRWZ010000000
VERSION     DASRWZ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44579"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..992)
                     /locus_tag="VFH60_08675"
     CDS             complement(168..992)
                     /locus_tag="VFH60_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HET6313896.1"
                     /translation="MDFKPKSEIRNPKSQFLYEVKDAVAVLTFNRPEVLNALTFEVYA
                     EFRDLLEELRYDDAVKVLVVTGAGDAFCSGGDVHKIIGALLEGDVKDHLAFTRMTGAV
                     VQNMRLLDKPIIAALNGMTAGAGAVIALASDLRIASDRTRFAFLFSRVGLAGVDMGSG
                     YLLPRVVGMGRATELLMLGDTIDAATADRYGLVNRLVPHDELMPTALDLARRLADGPT
                     QALAMTKRMLNNEWNMDLVSALEAEAQAQALMLMGDDHRRFYEAFQRKEKPQFGGR"
     gene            complement(1131..1928)
                     /locus_tag="VFH60_08680"
     CDS             complement(1131..1928)
                     /locus_tag="VFH60_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6313897.1"
                     /translation="MATDGDNLPLAGKRAVVTGAGRGIGRGIALALARAGADVALTAR
                     TAADLQPLAAEIEALGRRVVIAPCDVTDPQQVEAMSQRVLEAWGGLEVLVNNAGHAES
                     HKFVGHPDELWHEMLRTNLTSVYYVTKAFTPALIEARWGRIINIASTAARVGARYVAA
                     YTAAKHGVLGLTRALAVELLPHNITVNAICPGFVDTPMTGESIANIMARTGMSEQQAR
                     EALEKTSPQHRLIDPEEIAAMTVYLAGEMSKGITGQAINIDGGGVMS"
     gene            complement(1937..2614)
                     /locus_tag="VFH60_08685"
     CDS             complement(1937..2614)
                     /locus_tag="VFH60_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313898.1"
                     /translation="MNAQNIGSPEWEYTEVQVTDPAVVIRRHRKKGWELVSVVPVPVT
                     PSARRKPSPARRNLLLVAVAVGLLMLLVFGLTVLGGQPTGNEIPISQLVRDVRSGRVA
                     LIVTQEDSDRLTIYYGDPARKDTPVVTSVKEPGSNLHRYLSDAGVPQEALAQVDIRVE
                     QASSTGNYLGIAGFCLPLFFVIAVLVWWGWRLQARVQQWATLLIFRRPAPVRASTEEQ
                     AESARER"
     gene            complement(2667..3311)
                     /locus_tag="VFH60_08690"
     CDS             complement(2667..3311)
                     /locus_tag="VFH60_08690"
                     /inference="COORDINATES: protein motif:HMM:NF025391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HET6313899.1"
                     /translation="MELITPDLSRAFEDGRVVEVPIQEDTWRVRLERRELGHLVVASG
                     KVVGCDPFTVYEGVLPFDVEVEPGRYPVVLSIAHVEENGDQRVAYAMLSLGEGRPVRW
                     ELATWPGRGLADLGEGEVFGYPVDAGTGSFMDEVAARMLAQDEDLVDRLLDDSRDNYV
                     DTWTWTDFVADDDTGANVISFSSGFGDGFYPSYMAFDEDGRMVALVTDFGVVQD"
     gene            complement(3349..>3520)
                     /locus_tag="VFH60_08695"
     CDS             complement(3349..>3520)
                     /locus_tag="VFH60_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HET6313900.1"
                     /translation="CVDALFCIFLPPLASGVRARGCGAMLFVLLLTCIFWLPGSIMAF
                     IMTLNHNQERGR"
BASE COUNT          617 a         1181 c         1051 g          671 t
ORIGIN      
        1 agggccagaa acatgtgctc gacatcaagc tgcgtctgct gcctggcgaa cataatctgt
       61 tgcgcctcct gcaacgcctc ctgcgcccgc tccgtaaacc ggctcatcat aatctcacac
      121 tcttgatcct aacacgtaac acgtaactcg taattcgtaa ttacttgcta tcttccgccg
      181 aattgcggct tttccttgcg ttggaatgcc tcatagaagc ggcggtggtc gtcgcccatg
      241 agcatgaggg cctgggcttg cgcctcggct tcgagggcgg atacgaggtc catgttccac
      301 tcgttgttga gcatccgctt ggtcatggcg agcgcctggg tggggccgtc ggccaggcgg
      361 cgggccaggt ccaacgcggt aggcatcagt tcatcgtggg gcaccaggcg gttgacgagg
      421 ccgtagcggt cggcggtggc ggcgtcaatc gtatcgccca gcatgagcag ctcggtggcg
      481 cgccccatac ccaccacgcg cggcagcaag tagccggacc ccatgtccac ccccgccagc
      541 cccacccgcg aaaataggaa ggcgaagcgt gtgcggtcgg aggcgatacg caggtcggag
      601 gcgagggcta tcacagcccc ggccccggcc gtcataccgt tgagcgcggc gataatgggc
      661 ttgtccagca ggcgcatgtt ttgcaccacc gctcccgtca tccgcgtgaa agccaggtgg
      721 tccttcacgt ctccctcaag cagcgcccct ataatcttgt gcacgtcgcc gccggagcaa
      781 aaggcgtccc ccgcccctgt gacgactagc accttcaccg cgtcgtcgta gcgcagctct
      841 tccagcaggt cgcggaactc ggcatacacc tcgaacgtga gggcgttgag cacctcgggc
      901 cggttgaagg tgagcacggc cacggcgtcc tttacttcgt agagaaattg ggatttcggg
      961 tttcggattt cggatttggg tttgaagtcc acgacaactc ctcaagagac gatggacgat
     1021 ggacgattca gcagccgtca actactggca gcaccctaat aacagtcctt aaccttggcg
     1081 cgctcttggc gttcttggcg tcttggcggt tccgtctccc atcgtccaac ttatgacatt
     1141 accccgcccc cgtctatgtt gatagcctgc ccggtgatgc ctttgctcat ctcgcctgcc
     1201 aggtagacgg tcatggccgc gatttcctcc gggtcgatga ggcggtgctg cgggctggtc
     1261 ttttccaggg cctcgcgggc ctgctgctcg ctcatgccag tgcgcgccat gatgttggcg
     1321 atgctctccc ccgtcatggg cgtgtccacg aagccggggc agatggcgtt caccgtgatg
     1381 ttgtgcggca acagctccac ggccagggcg cgggtcaggc cgagcacccc gtgcttggcg
     1441 gcggtgtagg cagccacgta tcgcgccccc acccttgccg ccgtcgaggc gatgttgatg
     1501 atgcggcccc aacgtgcctc tatcaatgcg ggagtgaagg ctttggtcac atagtatacg
     1561 ctggtcaggt tggtgcgcaa catctcgtgc cagagttcgt ccgggtgccc cacgaacttg
     1621 tggctttcgg cgtggcccgc gttgttcacc agtacctcca gcccgcccca cgcctccagg
     1681 accctctgcg acatggcctc cacctgttgc gggtctgtca cgtcgcaggg cgcgatgacc
     1741 acgcggcggc ccagcgcctc tatctcggcg gcgagcggtt ggaggtcggc ggcggtgcgg
     1801 gcggtcaggg ccacgtcggc cccggcgcgg gccagcgcga gggcgatgcc gcggccaata
     1861 ccccgccccg cgcccgtaac cacggcccgc ttgcccgcga gaggtaggtt gtccccgtca
     1921 gtagccatag acaatcctat cgctcccgcg cggattctgc ttgctcttcc gtgcttgctc
     1981 tcacgggggc agggcgtctg aagattagca gggtggccca ctgttgcacg cgcgcctgga
     2041 gacgccagcc ccaccacacc aggactgcaa tgacgaagaa caggggaagg cagaagcccg
     2101 ctatgcccag gtagttgccc gtggaagatg cctgctcgac cctgatgtca acctgcgcca
     2161 gggcctcctg cgggactccc gcatcgctga ggtatcgatg caagttggac cccggttctt
     2221 tcacgctggt caccaccggt gtatccttcc gagcagggtc gccgtagtaa atcgttagcc
     2281 tatcgctgtc ttcctgggtg acgatcaaag ccactcgccc ggaccggacg tcccgcacaa
     2341 gctgagaaat cggtatctca ttgcctgtag gctgccctcc aaggacggtg aggccaaaca
     2401 ccagcagcat aagaagcccc acggccacgg caaccaataa taaattcctg cgcgcagggg
     2461 acggctttct gcgcgcggac ggcgttaccg gcaccggcac cacgctgacc agttcccacc
     2521 ctttttttct gtgccggcgg atcaccacgg ccgggtcggt tacctgcacc tcggtgtatt
     2581 cccattccgg cgacccaata ttctgtgcgt tcatggtagg gcctcatgct cgtgagcgtg
     2641 gcgctcgaag gcttgttccc ggcgggctag tcctgtacca cgccgaagtc cgtcaccagg
     2701 gccaccatgc ggccgtcctc gtcgaacgcc atgtacgagg ggtagaagcc gtctccgaag
     2761 ccggaggaaa acgaaatcac gttcgcgcca gtatcgtcgt ccgctacgaa gtcagtccag
     2821 gtccaggtat cgacgtagtt gtcccgcgaa tcgtccagca gcctgtctac caggtcctcg
     2881 tcctgggcga gcatgcgtgc ggctacttcg tccatgaagc tgccggtgcc cgcgtccacc
     2941 gggtagccga acacctctcc ttcgcccagg tcggccaggc ccctgccggg ccaggtagcc
     3001 agctcccagc gtacgggccg cccctcaccc aggctgagca tggcgtaggc tactctctgg
     3061 tcgccgttct cctcgacgtg ggcgatactc agcaccaccg ggtagcggcc cggctccacc
     3121 tccacgtcga acggcagcac gccctcgtac acggtaaagg ggtcgcaccc caccaccttg
     3181 cccgaggcca caaccaggtg gccaagctcc ctgcgctcca ggcgtacccg ccaggtgtcc
     3241 tcctggatgg gcacttccac gacgcgcccg tcctcgaacg ccctcgacag gtcgggtgta
     3301 atcagctcca tactgcctcc ccgctcgcag aacttccccc gcgacctatc aacgccctcg
     3361 ctcctggttg tggttgaggg tcataatgaa ggccattatg gagcctggca gccagaaaat
     3421 gcaggtcagc agcagcacga acagcattgc gccacaaccc ctggcgcgca ccccactggc
     3481 aagcggggga agaaatatgc agaataaagc gtcaacgcag
//
LOCUS       DASRWZ010000553         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1073039, whole genome shotgun sequence.
ACCESSION   DASRWZ010000553 DASRWZ010000000
VERSION     DASRWZ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073039"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1232
                     /locus_tag="VFH60_08700"
     CDS             9..1232
                     /locus_tag="VFH60_08700"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HET6313901.1"
                     /translation="MELVGENGSGEAYIIEALRTPVGRYAGALKDVRPDDLAAHVIAA
                     LVERTGVDARRIEDVILGCANQAGEDNRNVARMAALLAGLPVEVSGCTVNRLCGSGLQ
                     AVNDAARAIKSGEGEVFIAGGVESMTRAPFVMAKPSDAWPRGEQTLYDTTIGWRFTNP
                     RLAEMYYPYSMGETAENVAEQWGISREDQDAFAAESQRRAAAAIASGRFQEEIVPVSI
                     PQRKGDPIAVATDEHPRPDSTTEKLAKLGAAFKKGGSVTAGNSSGINDGASAVLLTSR
                     RAADEMGLKPLARVVTSAVAGVSPDCMGVGPVPATRKALERAGLTVDDLDLIELNEAF
                     AAQSLACIRDLRLDPEKVNVNGGAIALGHALGNSGSRLVTTLVHELKRRNGRYGLATM
                     CIGVGQGIATIIEMV"
     gene            1282..>2153
                     /locus_tag="VFH60_08705"
     CDS             1282..>2153
                     /locus_tag="VFH60_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6313902.1"
                     /translation="MADTQTASGTERKLWKLLINGNQVDAASGETFDVINPATNETLS
                     SVAKAGREDVDAAVAAAREAFDKGKWVRMGAARRASILYKVAQIMRDRSNEIAHLEVT
                     NNGKAIGQAQAELSQAIEDFEFFAGAATKIMGNTIPVPGNFLNYTVREPIGVCAQIVP
                     WNYPIMMAAWKLAPALAAGNTVVLKPASATPLTALLLGEICLEAGVPAGVVNVLPGPG
                     AEIGAYLAEHPGVDKIAFTGETETGRDIMRRAAGTLKRVTLELGGKSPNLVFDDADLD
                     HAVNGSLFAIYYSAG"
BASE COUNT          453 a          679 c          675 g          346 t
ORIGIN      
        1 ggaaggaaat agagttggtg ggagagaacg ggagcgggga agcatatatt atagaggcgt
       61 tgcgaacacc tgtagggaga tatgcgggcg cgctgaagga cgtgcggccc gatgaccttg
      121 ccgcgcatgt gattgccgcg cttgtcgagc gcaccggcgt agacgctcgt cgcatagagg
      181 acgtgatact tgggtgcgct aatcaggctg gcgaggacaa ccgcaacgtg gcccgcatgg
      241 ccgcgctcct ggccgggctg cctgttgagg tgagcggctg caccgtcaat cgcctgtgcg
      301 gttcaggttt acaggcggtg aacgatgcgg cccgcgctat caagtcgggc gagggcgagg
      361 tatttattgc tggtggcgtg gagagcatga cccgcgcgcc tttcgtaatg gcgaagccct
      421 cagatgcgtg gcctcgcggc gagcagacct tgtacgacac gacgataggc tggcgcttta
      481 ccaacccccg cctcgccgag atgtattacc cctacagcat gggcgagacc gccgagaacg
      541 tggcagagca gtggggcatc tcgcgtgagg accaggacgc cttcgccgcc gagagccaga
      601 ggcgcgccgc cgcagccata gcatcgggcc gtttccagga agagatcgtg cccgtcagca
      661 tcccgcaacg caagggcgac ccaatcgcag tcgctaccga cgagcacccc cgacccgaca
      721 gcactacgga gaaattagcc aaactaggtg ccgccttcaa aaagggtggc agtgtgaccg
      781 caggcaactc atccggcatc aacgacggcg catcagcggt gctcctgacg agccggcggg
      841 ctgccgacga aatgggcctc aagccccttg cgcgggtagt cacgtcggca gtggcaggcg
      901 tcagccccga ctgcatgggc gttggccctg tacccgccac gcgcaaggcg ttagagcgcg
      961 caggcctcac cgttgacgac ctcgacctga tagagttgaa cgaggcgttc gcggcgcaat
     1021 cactcgcctg catacgcgac ttgcgccttg accccgaaaa ggtcaacgtg aacggcgggg
     1081 caatcgcgct gggccatgcg ctaggcaata gcggctctcg cctcgtcacc accctcgtac
     1141 acgaactgaa gcgccgaaac ggacgctacg gcctcgccac catgtgcata ggcgtcgggc
     1201 agggcatagc caccataatc gagatggtct agctgaagca ccccgcgctg taataattca
     1261 ccccaggagc aaccaatact tatggccgac actcaaaccg cgtcaggaac ggagcgcaag
     1321 ctatggaagc tgctcatcaa tggcaatcag gttgacgccg cttcgggcga gaccttcgac
     1381 gttatcaatc ccgcgaccaa cgaaacgctc tcatccgtgg cgaaagccgg gcgcgaagat
     1441 gttgacgctg cggttgctgc cgcccgcgag gcgttcgaca agggcaaatg ggtgcgtatg
     1501 ggcgcggctc gcagggcgag catactctac aaagtagccc aaataatgcg cgaccgcagc
     1561 aacgaaatag cccaccttga agtgaccaac aacggcaagg ctatcggcca ggcacaggca
     1621 gagttgagcc aggccataga ggacttcgag ttcttcgcgg gcgcggccac caagataatg
     1681 ggcaacacaa tccccgtgcc tggcaacttc ctgaattaca ccgtccgcga gcctataggc
     1741 gtgtgcgccc agatcgtccc ctggaactac cctatcatga tggccgcctg gaagctggcc
     1801 cccgcgctgg ctgcgggcaa tacggttgtg ctcaaacccg caagcgccac tcccctgacc
     1861 gccctcctcc tgggcgagat ttgcctggaa gcgggtgtcc ctgctggcgt ggtaaacgtc
     1921 ctgcccggcc ccggcgcgga gataggtgcg tacctggccg agcacccagg cgttgacaag
     1981 atagccttca ccggcgagac cgagacggga cgcgacatca tgcgtcgcgc ggcaggcaca
     2041 ctcaagcgcg tcacccttga gcttggcggc aagtcgccca acctcgtctt cgatgatgcc
     2101 gacctcgacc acgcggtcaa cggctccctc ttcgccatct actacagcgc ggg
//
LOCUS       DASRWZ010000554         4161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1210813, whole genome shotgun sequence.
ACCESSION   DASRWZ010000554 DASRWZ010000000
VERSION     DASRWZ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210813"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFH60_08710"
     CDS             complement(<1..973)
                     /locus_tag="VFH60_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313903.1"
                     /translation="MPKTPPQFKQAAIPRNPGSMPYVRVAPCGRYFETEDGQPFLVVG
                     HNDAMPWPGMHNLHYEQDIATTEGYIKDLVAHGVTVMRVMLEYVQDRHWLFERPVGNY
                     LPEAVLYWDDLIGLCERHGMRLLVLFWDTFFLSRRWKHHPYSRKGTGFDGQWSMCTSP
                     QGMEHEKARIRFFIDRWGNSPAIFGYDLFNEIHPHWGGTPEDQHRWITEMARFVKGYE
                     LEKWGKRHLLTVSVFGAFPEGGYIDLIYRHPELDFATTHVYQNDAKDNPKDTTSGAIT
                     MRDAVRFAYANMAGVRPYMDTESGPIFGYMHTRKRPTAEFENEYYHNM"
     gene            complement(1020..2492)
                     /locus_tag="VFH60_08715"
     CDS             complement(1020..2492)
                     /locus_tag="VFH60_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313904.1"
                     /translation="MLEQVSTFREAAVPRDRAGMPYVGLAPGGRYFMTEDGKPCLAVG
                     HNEALPWPRMWHMQDERDIPTVESYIKMMAEHGVTVVRIMLEYCEDEQWFFEDAEGRP
                     VPSTVQYWDNLIGLCERYKIRLLVQFWDTFFMSRRWEHHPYSAPGSGFDGPGSFCTSP
                     TALEAQKRRIRFFIDRWGDSPAIFAYDLLNEIHPYWGGTPEEQADWLAEIARFTRDCE
                     LERWGKRHLLTVSIFGARPDEGYLDLIYRHPELDFASTHVYELGVVDNPENTIDCAVV
                     MRDAVKYAFRHMSGTRPYMDSESGPIHLFMDLKLFLQEPFESEYYHNMSWAHLATGGA
                     GSGLRWPFRDPHNLTQEMHEVQRGMSRFVASAWECGFDWLNFSPQPIDEGMQVVSRTP
                     GAEGPLPVLPFGCSDGVHSLMWLLRDKRVAEDDAPIAPADLLVQRPHEGRFIAEFWET
                     YSGERLGEYRFEVANSSSIRLPLPAFGTGIAITLRPASTE"
     gene            complement(2600..3427)
                     /locus_tag="VFH60_08720"
     CDS             complement(2600..3427)
                     /locus_tag="VFH60_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HET6313905.1"
                     /translation="MTVHQYTKLSSNGLQSEIDALGPWFHNLHLPDGTQTAPAHPLGD
                     FPSYKWGKLAPHLPEDLAGWTALDIGCNAGYYSFELARRGANVTGIDVDQHYLDQAAW
                     AARQFGLEDRVSWKRMQVYDLARTGEQYDLVLFMGVLYHLRYPMLGLDIVTQKVKRLM
                     VFQTLTMPGMEVYEDTDQLDIEHREAMQEPGWPKMAFIEHRFANDPTNWWAPNHAGVE
                     AMLRAAGMRVTERLGHEIYLAEPDPERPSCMTTWNKDEFLSATGQERSAEYRVPSSE"
     gene            complement(3534..4076)
                     /locus_tag="VFH60_08725"
                     /pseudo
     CDS             complement(3534..4076)
                     /locus_tag="VFH60_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239385.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          716 a         1268 c         1333 g          844 t
ORIGIN      
        1 tcatgttgtg gtagtattcg ttctcaaact cggcggtagg gcgcttgcgg gtgtgcatgt
       61 agccgaagat tgggccgctc tcggtgtcca tgtaggggcg cacgccggcc atgttggcgt
      121 aggcgaagcg caccgcgtcg cgcatggtga tggccccgct ggtggtgtct ttcgggttat
      181 ccttcgcgtc gttctggtag acgtgagtgg tggcgaagtc gagttccggg tggcggtata
      241 taaggtcgat gtagccgccc tcggggaacg cgccgaacac cgacacggtg agcaggtgcc
      301 gcttgcccca cttctccagc tcgtagcctt tgacgaaccg cgccatctcg gttatccagc
      361 ggtgctggtc ttccggcgtg ccgccccagt gcgggtgtat ctcgttgaac aggtcgtagc
      421 cgaagatggc gggcgagttg ccccagcggt cgatgaagaa gcggatgcgg gccttctcgt
      481 gctccatgcc ctgcgggctg gtgcacatgc tccactggcc gtcgaagccg gtgcctttac
      541 ggctgtaggg gtgatgcttc cagcggcgcg ataggaagaa ggtgtcccag aagagcacca
      601 gcagccgcat gccgtggcgc tcgcacaggc cgatgaggtc gtcccagtag agcacggcct
      661 ccggcaggta gttgcccacc ggccgctcga agagccagtg gcggtcctgc acgtattcca
      721 gcatgacgcg catgaccgtg acgccgtgtg ccacgaggtc cttgatgtag ccctctgtgg
      781 tggctatgtc ctgctcgtag tgcaggttat gcatgccggg ccagggcatg gcgtcgttgt
      841 gcccgaccac caggaaaggc tggccgtcct cggtctcgaa gtaccgcccg caaggagcta
      901 cccgcacgta gggcatgctg ccggggttgc gcgggatagc ggcttgcttg aattgtgggg
      961 gggtcttggg catgatacgg attttggatt tggttatggc tcttggcact tcaactcagt
     1021 tactcggtgc tggctggacg tagtgtaatg gctatgccgg tgccgaaggc gggcagcggc
     1081 aggcggatgg atgaagagtt ggccacttcg aacctgtact cgcccagccg ctcgccggaa
     1141 taggtttccc agaactcggc tatgaagcgg ccttcgtggg ggcgctgcac gaggaggtcg
     1201 gcgggggcta tgggcgcgtc gtcctccgcc acacgcttgt cgcggaggag ccacatcagg
     1261 gagtggacgc cgtcagagca gccgaagggg aggacaggca gcggaccctc ggcccccggc
     1321 gtgcggctca ccacctgcat tccctcgtca attggctgcg gcgagaagtt gagccagtcg
     1381 aagccgcact cccacgcgct cgccacgaat cgggacatgc cgcgctgtac ctcgtgcatc
     1441 tcctgcgtga ggttgtgcgg gtcgcgaaag ggccagcgca ggccgctccc cgccccgccc
     1501 gtagccaggt gcgcccagct catgttgtgg taatactcgc tctcgaacgg ctcttgcaag
     1561 aacagcttca ggtccatgaa caggtggatg gggccgctct cgctatccat gtaggggcgg
     1621 gtgcccgaca tgtggcggaa ggcgtatttc accgcgtccc gcatgactac cgcgcagtcg
     1681 atggtgttct ccggattgtc caccacacct aattcgtaga cgtgggtgct ggcgaagtca
     1741 agctcagggt gccggtagat gaggtctagg tagccttcgt ccggcctggc accgaatatc
     1801 gacacggtga gcaggtgccg cttgccccac cgctcaagct cgcagtcgcg ggtgaaccgc
     1861 gctatttctg ccagccagtc cgcctgttct tccggggtgc cgccccagta ggggtggatt
     1921 tcgttgagca ggtcgtaggc gaagatagcg ggagagtcgc cccagcggtc gatgaagaag
     1981 cggatgcggc gcttctgggc ctcaagggcc gtgggcgagg tgcagaagct gcccggccca
     2041 tcgaagccgc tacccggcgc gctgtagggg tggtgctccc agcggcgcga catgaagaag
     2101 gtatcccaga actgcaccag cagcctgatc ttgtaccgct cgcacaggcc gataaggttg
     2161 tcccagtact ggaccgtgct cggcaccggc ctgccctcgg cgtcctcgaa gaaccattgc
     2221 tcatcctcgc aatattccag catgatgcgc acgaccgtaa ccccatgctc cgccatcatc
     2281 ttgatgtagc tctccacggt ggggatgtcg cgctcgtctt gcatgtgcca catgcgcggc
     2341 cacggtaacg cctcgttatg ccctaccgcc aggcacggct taccgtcctc ggtcatgaag
     2401 tagcgtccgc ccggagccag ccccacgtaa ggcatgccgg cccggtcgcg tgggaccgca
     2461 gcctctctaa aggttgatac ttgctccaac attgtccttg ataccctttc ctggggaagt
     2521 gatgagtacc gagtaccgag taccgagtgt catctatcct aaactttgcg cgtgctttgt
     2581 gcctttgcgg tttccgtctc tactcggaac tcggcactcg gtactcggca cttctttcct
     2641 gccccgtagc ggataggaac tcgtctttgt tccaggtggt catgcatgag gggcgttcgg
     2701 ggtcgggttc ggccaggtag atttcgtggc ccaggcgctc cgtgacgcgc atgccagcgg
     2761 cgcgtagcat ggcttccaca ccggcgtggt tgggcgccca ccagttggtg gggtcgttgg
     2821 cgaagcggtg ctctatgaag gccattttgg gccagcccgg ctcctgcatc gcctcccggt
     2881 gctcgatatc aagctggtcc gtatcctcgt atacctccat gcccggcatg gtgagcgtct
     2941 ggaacaccat aagccgcttc accttctgcg tgacgatgtc gaggccgagc ataggatagc
     3001 gcaggtggta gagcacgccc atgaagagca cgaggtcgta ctgctcgccg gtgcgagcga
     3061 ggtcgtacac ctgcatccgc ttccagctca ctctgtcctc aagcccgaac tgtcgagccg
     3121 cccaggctgc ctggtccagg taatgctggt ccacgtcgat gcctgtgacg ttcgctcctc
     3181 gccgggcaag ctcgaagctg tagtagcccg cgttgcagcc gatgtcgagg gcggtccagc
     3241 ccgcaaggtc ttccggcagg tgcggggcga gcttgcccca cttgtacgag gggaagtcgc
     3301 cgaggggatg ggcgggggcg gtctgcgtgc cgtcgggtag gtgcaggtta tggaaccagg
     3361 ggccgagcgc gtcaatctcg ctctgcaagc cgttagacga gagttttgta tattggtgaa
     3421 ctgtcatatc tctccaccat gctagagtaa gtttgagtct gtgagaacag ccacgccata
     3481 ggcacgcggc ttactaagca atgtaaaggc catatccgca cgcgccgcac tcgtcacagc
     3541 atgtccggca caacccgctt gccgagcaac tcccgcgcgt atgcctcaag ctcctgcgcc
     3601 cggtgtgccg acgtatgatg ctgcaacact cgttgcctgg cacgctcacc aatgcgcagc
     3661 cgttcctcct cgggaaggtc gcgcaggtaa gacagcgtgt cctccggcga gcgggccacc
     3721 agtatctccg tgccgaactc gaatatggtg tccaacccct cccagtagtc gctgatgatg
     3781 ggcacgccgc acgccgccgc ctcgaacagg cgcacgctag gcgagtagcc cgcgctgacc
     3841 atgtcggccc gcgtgatgtt gagggtgaag cgttgcgcgt tgtagaactc ccggtgctcg
     3901 gcgggcggca aatgctccac ccgccgcacg ttccgcggcc acacgatatg ctcagggtac
     3961 tgcggccccg ccaccacgaa cctgccctcc gcccaccgcc gcgcaggctc caacatcagc
     4021 aaatcgagcg gaggctgccg gtcgtcgcta taagtaccca tgtagcccat gtcataggat
     4081 ttggaatttg gaatttcgga tttcgaattt gctcggaatt catctgaatc cgaaattcca
     4141 aattccaaat tcaaaatcgg g
//
LOCUS       DASRWZ010000555         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_319891, whole genome shotgun sequence.
ACCESSION   DASRWZ010000555 DASRWZ010000000
VERSION     DASRWZ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319891"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1093)
                     /locus_tag="VFH60_08730"
     CDS             complement(104..1093)
                     /locus_tag="VFH60_08730"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET6313906.1"
                     /translation="MAKYAAITGWGSAVPERVLTNFDLEKIVETSDEWITSRTGIRQR
                     HVLSEGETTSTLAVAASRQALEKAGVSPWQVDLVICCTATPDFLFPATACLVQHEIGA
                     ENAGAFDLEAACSGFVYGLSVGSQFIKAGSYKTVLVVAAEGLSRFLDWQDRTTCVLFG
                     DGAGAVVLEATDRKQGLLSFVLGSHGVGGDLIKLPGGGATIPASIESLENREHFIKMN
                     GQEVYRFAVRIMGDSAVEALDKASLEYDDISLFIPHQANTRIINSIADRLHLDPEQVF
                     NNIANNGNTSAASIPIALCEAVDEGKVKDGDNVLFVAFGSGLTSAAAVVRWGR"
     gene            complement(1176..1376)
                     /gene="rpmF"
                     /locus_tag="VFH60_08735"
     CDS             complement(1176..1376)
                     /gene="rpmF"
                     /locus_tag="VFH60_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET6313907.1"
                     /translation="MGATPKQRISSKHRGDRRSQQALKPIQLVECPQCHNPRLPHHVC
                     PTCGTYRGRQVLQVKGTESGQA"
     gene            complement(1441..2040)
                     /locus_tag="VFH60_08740"
     CDS             complement(1441..2040)
                     /locus_tag="VFH60_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET6313908.1"
                     /translation="MDVTNSNADLVFNVAQLLKERVGSTRKFHIDTPNLVLDAEPGEG
                     EALHAQKVRGDVKVTRLADSLLVQGDIDADVLLECSRCLETFTRPVDARLEEQFLPTV
                     DVDTGAPVRREEFEENDLAFDIDPNHLMDLTEPVRQALLVALPMKPLHSEDCKGICLQ
                     CGANLNEVDCGHSDEPVDDRWTGLRELRVEDFPAGDNVN"
     gene            complement(2156..>2829)
                     /locus_tag="VFH60_08745"
     CDS             complement(2156..>2829)
                     /locus_tag="VFH60_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET6313909.1"
                     /translation="FAADWRRLIVAFFASVFERRIGDDTDRRLVWLIVLGTIPAALAG
                     MLLEGSIDTVFHDPRNLRTGLLVIAVMMIVMGALLLFAERVGKRTIPIEGVRLPTAMA
                     VGVAQALALIPGVSRSGSTITAGLFAGLKRDAAARFSFLLSTPVVLAAGVKQVYDLTK
                     EGGLPASDQLGFFVGFVASAVSGFVCISFLLRYLQRHSTAPFIWYRFMVGAALLALVL
                     VGFRA"
BASE COUNT          518 a          930 c          878 g          503 t
ORIGIN      
        1 cttgcccatg cccacggctt gcgaaccctg gccggggaac acccaggccg tagcggacgg
       61 tcccgcaccg tgccgtgcaa ccgccgccgg gtcgctcatg cccctaccgc ccccaccgca
      121 cgacggcggc ggcactggtc agcccggagc cgaaagccac gaacagcacg ttgtcgccgt
      181 ccttgacctt gccctcgtcc accgcttcac acagcgcgat agggatagag gccgccgagg
      241 tgttgccgtt gttggcgatg ttgttaaaga cctgctccgg gtctaggtgc aggcggtcgg
      301 ctatcgaatt gatgatgcgg gtgttggcct ggtgggggat gaagagggat atgtcgtcgt
      361 actcgaggct ggccttgtcg agcgcctcca ccgccgagtc gcccatgatg cgcaccgcga
      421 agcggtacac ttcctgcccg ttcatcttga tgaagtgctc gcggttttcc aggctttcga
      481 tgctggccgg tatggtagcg ccgccgcccg gcagtttgat gaggtcaccg cccacgccat
      541 gcgagccgag cacgaacgac aggaggccct gcttgcggtc ggtggcttcg agcaccacgg
      601 cccccgcgcc atccccaaac agcacgcagg tggtgcggtc ctgccaatcg aggaagcgcg
      661 acagcccctc cgcggccacc acgagcaccg tcttgtacga acccgccttg atgaactgcg
      721 agccgacgct cagcccgtac acgaagccgg agcaggccgc ctcaagatcg aaggccccgg
      781 cgttctcggc cccgatctcg tgctggacga gacacgcggt cgccgggaaa aggaaatcag
      841 gggtagcggt gcagcagatg acgagatcga cctgccaggg ggacacgccc gccttctcaa
      901 gcgcctgccg ggaggctgcc acggcaagcg tggaggtggt ttccccttcg ctgagaacgt
      961 ggcgctggcg aatgcccgta cgagaggtaa tccactcgtc cgaggtctcc actatctttt
     1021 cgaggtcgaa gttggtgagt accctttcgg gcaccgcgct gccccagccg gttatagccg
     1081 cgtactttgc cacaattcgg tagctcccta tcgtctcacg cccggagata tagagttata
     1141 gctgcgcccg acgctggacg cggccggccc tggctctatg cctggccgga ctcggtgccc
     1201 tttacctgga ggacctggcg gccgcgatag gtgccgcagg tcgggcatac gtggtgtggc
     1261 aggcgcggat tgtggcactg cgggcactcg accaactgga tgggcttgag ggcctgctgg
     1321 ctgcggcggt cgcccctgtg cttgctggaa attctctgct tgggtgtcgc acccataatg
     1381 tttgttctcc tctacagcta cttgctaacc gtttcttagt tagctcgata atagaaaact
     1441 ctaattgacg ttgtccccgg ccggaaagtc ttcgacgcga agctctcgaa gcccggtcca
     1501 cctgtcgtcg acaggctcgt ccgagtggcc gcaatcgacc tcgttcaggt tggcaccaca
     1561 ctgcaagcag atgcctttgc agtcctcgct gtggagcggc ttcatcggga gggccaccaa
     1621 cagcgcttgg cgcaccggct cggttaggtc catcaggtgg ttcgggtcga tgtcgaacgc
     1681 caggtcgttc tcctcaaact cctcgcgccg caccggggca cccgtatcca cgtccacggt
     1741 gggcaagaac tgctcttcga ggcgggcatc cacgggccgc gtgaacgttt ccaggcaccg
     1801 gctgcattca agcagcacgt cggcgtcgat gtcgccctgc acgagcaggc tgtcggccag
     1861 gcgcgtgacc ttcacatcac cgcgtacctt ctgcgcgtgc aaagcctcgc cctcacccgg
     1921 ctccgcatcg agcaccaggt tcggagtgtc gatgtggaac ttgcgagtgg agccgacacg
     1981 ctccttgagt agctgggcta cgttgaaaac caggtcggcg ttggaatttg taacgtccac
     2041 gttctaccac tttcaaatga atttacaaat gcctccattt acgaggcaac ttcgctatta
     2101 tatggcacgg caaagcgcgt gtcaagcgaa gccgctcccg tgcgggttga ccggctcagg
     2161 cacgaaagcc caccagcacg agcgccagga gcgccgcgcc aaccatgaag cggtaccaga
     2221 tgaaaggggc ggttgaatgc ctttgcaggt agcgcagcag gaaggagatg cacacgaagc
     2281 ccgacaccgc cgaggccacg aaccctacga agaaacctag ctggtcgctc gcgggtaggc
     2341 cgccctcctt cgtcaggtca tacacctgct tcaccccggc cgcgagcacc acgggcgttg
     2401 agaggaggaa ggagaagcgc gccgccgcat ctcgcttcag ccccgcgaac agtcccgccg
     2461 ttatggtgct gccggagcgc gacacgccgg ggatgagggc cagcgcctgg gccacgccca
     2521 cggccatagc ggtgggcagc ctcaccccct cgatcgggat ggtgcgcttg cccacccgct
     2581 cggcaaacag cagcaacgca cccatgacga tcatcatcac cgctatcacc agcagccccg
     2641 tgcgcaggtt acgcgggtcg tgaaacaccg tgtctatgct gccctccagg agcattccgg
     2701 ccagcgccgc cgggatggtg ccgagcacga tcagccagac gagccgccgg tcagtgtcgt
     2761 cgccaattcg ccgctcgaac acggaggcga agaacgccac gatgagcctg cgccagtcgg
     2821 cggcgaagt
//
LOCUS       DASRWZ010000556         6770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_662589, whole genome shotgun sequence.
ACCESSION   DASRWZ010000556 DASRWZ010000000
VERSION     DASRWZ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662589"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..713)
                     /locus_tag="VFH60_08750"
     CDS             complement(135..713)
                     /locus_tag="VFH60_08750"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HET6313910.1"
                     /translation="MNSIGTKRAGRVFYRVVILVAALLLVAVVGPRVASAHAHLESST
                     PADGATVASGLTQVTVTFSEEVSVDQSSAQLASADGSAVSGASGAVDRANRNTMVITT
                     PALADGKYTVTWRAVTEDDNGITNGTFSFTVGTGGGTTAGTTTTGGTTGTGGASLPAT
                     GSGDALPLTLLGLLLATLLLGAGTLAKRAARA"
     gene            complement(788..1822)
                     /locus_tag="VFH60_08755"
     CDS             complement(788..1822)
                     /locus_tag="VFH60_08755"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET6313911.1"
                     /translation="MVNFTRTRILAALAWLVLLPVASACLPSQPNEETASEAAVRQRP
                     TPDGRVVTADQASNTLTLIDVATNEVYGIIKTGQQPHHVVASPDGKELWVSLYGEHRL
                     QVFDAVTLAELASVDVEASNDDLVFDPSGKMLYVSLGSTDAVAVVDVAARKMVKTVKV
                     GKTPHGVRTSPDGNLLLVTNTADNTVSVLSLQPEAAVKTTIETGPNPFEVYISEDSKT
                     AYVSNFLGDSISVLDLVENRRGAFIRSGKQPAMLILQGGAGAEKLWVANTGAGEVWAI
                     DPATRKLITRVTVGKGAHGVVATPSGKLFVTNSTDNTLSVVDSSTNQVLATLQVGSNP
                     NGLTYVPNAQ"
     gene            complement(2010..4373)
                     /locus_tag="VFH60_08760"
     CDS             complement(2010..4373)
                     /locus_tag="VFH60_08760"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET6313912.1"
                     /translation="MKAATVGGARRWLFPALVMALLWVWLYAPSPALAHAKLLKSSPT
                     AGALLDAPPEKVELSFSEGVSLQFSSMKLLDRSRKELAMGALGHEGQGDTYVAASLPS
                     GLAPGTYTVVWRVLSAVDGHVTTGNLAFRVRPPAGSQGTTEPGGSAEAPPEEVVPVSP
                     VEGSTETPDPFRWLTRSLLLASAALLVGGAIFSALVVRPTLANDPGGPALLPVLRRGL
                     GRAGTIAAAVLLLALALDLVLQIGSIVGGGLGVGLQNLALAGTLITSTGYGYAWIVKV
                     AAGAALLAVMLVAWRAGQPSEGKAPLPVWSIATLAGVGLALGEALSSHAAASGSGSSA
                     ASGGTLVSGGVIPLPLLSDWVHLTTAFTWAGGLGYMALVLYPAFRSSGLSSEEQRAVL
                     GRAVPRFSRVALLSVAILAVTGTYNALVHTTDLGAFATSAYGQVLILKIALFAFLMPL
                     GAINLLKLTPLLRRKEQTGSPGSPTGRTTGVDPTRSLRRTVRGEVVLVVGALVCAAGL
                     SLLPPPSDAEGAYTASPNATTVAAAPTTPVTQPTPTEPVPLTASAQTGVQGYRVALDV
                     TSSFEGDELTATVGRADPAAVPLTDVRKVIFKVTPQDVDGGSTALEAEAEAGGGGDNQ
                     VWKANETVLTLDGGYMVTVIVQRTQAPDLKAAFRLDLSLDTGLKARASEVLEIRLDTV
                     PSPPISGTATLKLMLLDGTGVPVSDATITVSPLMPAHGHIEPTDVAKAVPGEPGAYTM
                     PINFQMGGSWLIIFNVERPGKDTIKVDAGLEVIDPNATPTPEVELEE"
     gene            complement(4501..5955)
                     /locus_tag="VFH60_08765"
     CDS             complement(4501..5955)
                     /locus_tag="VFH60_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313913.1"
                     /translation="MPIGALLMVLAAAIFTYQIVTQSQAGTGVQNGNGNEDGPVTLEY
                     TTEQGKDSRLGTVNSPLRITLRVPYNGIEDSMISTASVQLFDEAGKPAEYGGTPAEPF
                     VLQATYELGVWAYNTSMPSKPGKYTGRVQINYRATGGPAPTPQSYDLSNTVLEALAEN
                     GPPLSSGYVFTKDANLWILSTDTVKQRRLTFFPVESEYANDATWSPDGKLIAFAHTPK
                     SDPTELPTSDIWVINADGTGAQIVVQHQEGETLYDPTFSADGKYLYFTAEKQDMASTN
                     YDANGMPIGLRGIDRYDLAAGERTHWKDNSHNPVEGGTDGALMYQGTPDAVSTDAGAA
                     PGVKLVREKPDGSLSQVLVDSSAYTAFYGPASSPDGSKVAFAAIEVPPAVPGGGALDI
                     LKWLTFEPEVAYAHGLPWDLYMVPGNGGGKAVRLTNMAEDQPHAAWIDNNTLAFMGVS
                     GLYKLQLGPDGNPTGKPQKIHDGAPHGGLTWHGP"
     gene            6225..6473
                     /locus_tag="VFH60_08770"
     CDS             6225..6473
                     /locus_tag="VFH60_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313914.1"
                     /translation="MGHQHDDENVHDMGQGHSGKGDKGHAGDKDHEAGGGRREHRASG
                     SHESNITDTKNDSSSSTSGGGSSGGGGGSHGGGTKENQ"
BASE COUNT         1184 a         2048 c         2243 g         1295 t
ORIGIN      
        1 ctgtttagca aagcaaaagg gcagcatcgt acagtagatg cggccctttt gctttaccaa
       61 ccgcagtact tctcggcgtg ccctctgcgt cctctgcgtc ctttgcgtct ctgcggtgga
      121 ttaatcagct gggactaagc ccttgctgcg cgctttgcca gcgtgcccgc gccgagtagg
      181 agggtggcga ggaggaggcc aaggagggtc agcgggaggg cgtcgccgga gcctgtggcg
      241 gggagggacg cgccacccgt accagtggtg ccgccggtag tagtggtgcc ggcggtggtc
      301 ccgccacccg tacctactgt gaagctgaag gtgccgttgg tgatgccgtt gtcgtcctcg
      361 gttacggccc tccaggtgac ggtgtacttg ccgtcggcca gtgctggggt ggtgatgacc
      421 atcgtgttgc ggttggccct gtccaccgcg ccgctagctc cggacacagc gctgccgtcg
      481 gctgaagcca gttgcgcgct cgactggtct acgctcacct cctcgctgaa ggtgactgtg
      541 acctgggtga ggccggaagc tacggtggct ccatccgccg gggtcgagct ttcaagatga
      601 gcatgggccg atgcgacccg tggccctacc accgctacca gcagcagcgc cgccaccagg
      661 ataacaaccc tgtagaacac tctccccgcc ctctttgtgc ctattgaatt catgtgtcgg
      721 tactctcctc tttgtgaatt gttgccatca tgaccggccc atcctgtttg ccataccggt
      781 attatagcta ctgcgcattg ggaacgtaag tgaggccgtt cgggttgctg cccacctgca
      841 acgtcgcgag cacctggttg gtgctggagt ccactaccga cagggtgtta tcggtggagt
      901 tggtgacgaa cagcttgccg gagggtgtgg ctaccacgcc gtgcgctccc ttgcccacgg
      961 tgacgcgggt aatcagcttg cgtgtggccg ggtctatggc ccaaacttcg cccgcgcctg
     1021 tgttggcgac ccaaagcttc tcggcacccg cgccgccttg caggatgagc atggcgggtt
     1081 gcttgccgct cctgatgaac gcgcccctcc tgttctctac gaggtccagc accgaaatcg
     1141 agtcgcccag gaaattgcta acgtaagcag ttttgctgtc ctcgctgatg tacacctcga
     1201 acgggttggg gccggtctct atagtggtct tgaccgctgc ttcgggttgg agggacagca
     1261 ccgagacagt gttgtcggcg gtgttggtga cgagtaacag attgccgtcc ggggaggtgc
     1321 gcacgccgtg gggggtcttg ccgaccttga ctgtcttaac catcttgcgt gcggccacgt
     1381 ccaccacagc cacagcatcg gtgctgccga gcgacacgta cagcatcttg ccggaggggt
     1441 cgaataccag gtcgtcattg ctcgcttcca cgtccaccga agctagctcg gcaagcgtta
     1501 cggcgtcgaa cacttgcagg cggtgctccc cgtagagaga cacccacagt tccttgccgt
     1561 cgggagaggc gaccacgtgg tgcggctgct gacccgtctt gatgatgccg tagacctcat
     1621 tggtggctac gtcaatcaga gtgagggtgt tggatgcctg gtccgccgtg acgacgcggc
     1681 cgtcgggtgt gggcctctgt cgtacggcgg cctcggaggc ggtctcttcg ttcggctgcg
     1741 acggcaggca tgccgaagcg accggcaaaa gcaccagcca agccagtgcg gctaatatcc
     1801 ttgtgcgcgt aaaatttacc atagataacc tcgctctagt cgagtaatga gtgatgagga
     1861 ctgagcactg agtgaaggga gaggccccgt ggctgaagcg acgggctggg aagggtgcag
     1921 cgacctggct ccttgttcct ttgtcaatcg tgagcagggc aattctccag cctcagtcct
     1981 cagtcctcat cactcattac tcatttctgt cactcctcca actccacctc cggcgtgggt
     2041 gtggcgttgg ggtctattac ttccagcccg gcatcgacct tgatcgtgtc cttgccgggg
     2101 cgttcgacat tgaatatgat aagccatgag ccgcccatct ggaaattgat gggcatagtg
     2161 taagcgcccg gctcgcccgg tacagctttg gctacgtccg ttggctcgat gtggccgtgg
     2221 gcgggcatga gcgggcttac cgtgatggtg gcgtcgctga caggcactcc cgtgccgtcc
     2281 agcagcatca gcttcagggt ggcagtgccg ctgattggcg ggctgggcac cgtgtctagc
     2341 cgtatctcca gcacctccga cgccctggcc ttcagccccg tgtccaggga gagatcgagg
     2401 cggaaagcgg ccttgaggtc gggggcctgg gtgcgctgca cgatcaccgt gaccatgtag
     2461 ccaccgtcca gggtcagcac cgtttcgttg gccttccaca cctggttgtc cccgccgccg
     2521 cctgcctccg cttccgcttc gagggccgtg ctccccccgt ccacgtcctg cggcgtcacc
     2581 ttgaagatga ccttgcgcac gtctgtgagg ggcacggctg ccgggtccgc tctcccgaca
     2641 gttgcagtca gttcgtcgcc ctcgaacgag gaggtgacat ccagggccac gcggtagccc
     2701 tgcacgccgg tctgcgcgga ggcggtgagg ggcacgggtt cggtgggcgt gggctgcgtc
     2761 acgggggtag tgggtgcggc ggccacggtg gtggcgtttg gggaggcggt gtaagctcct
     2821 tcggcgtcgc tgggcggcgg aaggagggag aggcccgccg cgcaaaccag cgcccctacc
     2881 accagcacta cctctccacg cacggtgcgg cggaggctac gcgtggggtc tacgccggtc
     2941 gtccggcccg tgggtgagcc tggtgagcct gtttgctcct tgcgcctgag cagcggggta
     3001 agcttgagca ggttgatggc cccgaggggc atcaggaagg cgaacagggc tatcttgagg
     3061 atgagcacct gcccgtaggc gctggtagcg aacgccccca ggtcggtggt gtgcaccagc
     3121 gcgttatagg tgccggttac tgccaggata gccacgctga ggagcgccac gcgggagaag
     3181 cgcgggacag cgcggcccaa cacggcccgt tgctcctctg aagataggcc gctgctgcgg
     3241 aaggcggggt agagcaccag ggccatatag cccaggccgc ccgcccacgt gaaagcggtc
     3301 gtgaggtgca cccagtcgct cagcaggggc agggggatga caccgccgga caccagcgtg
     3361 ccccccgagg ccgccgaact accgctgccc gaggcggcgg cgtggctgct gagcgcctcg
     3421 cccagggcaa gccctacccc cgccaaagtc gctatggacc ataccgggag cggggccttg
     3481 ccctccgacg gctgcccggc tcgccaggcc acgagcatga cggccagtag cgccgcgccc
     3541 gccgccactt tgacgatcca ggcgtacccg tagccggtgc tggtgatgag cgtgcccgcc
     3601 agcgccaggt tttgcaggcc gacgccgagg ccgccgccca cgatgctgcc gatttgcaac
     3661 accaggtcca gggccagcgc cagcaggagc accgccgccg cgattgtgcc ggccctgccc
     3721 agcccgcgtc tcagtacggg cagaagggct gggcctccgg ggtcgttcgc cagggtgggc
     3781 cggacaacca gggccgagaa gatggcccca cccacgagca aggccgcgct cgcaaggagc
     3841 agagagcggg tgagccagcg gaacgggtcg ggggtttcgg tgctgccctc cacgggcgag
     3901 acgggcacga cttcctcagg cggcgcttcg gcggacccgc ccggctcggt cgtgccttgc
     3961 gaaccggcgg gcgggcgcac cctgaatgcc aggttgccgg tcgtgacgtg tccatccacc
     4021 gccgacagca ccctccacac gacggtgtaa gtgccgggtg cgaggccgct gggcagggag
     4081 gcggccacat atgtgtcgcc ttgcccctcg tgccccagcg cgcccatcgc cagttccttg
     4141 cgcgaacggt cgagcagctt catggagcta aactgtaagc tcacgccctc gctgaacgaa
     4201 agctctacct tctcaggggg agcgtccagc agcgcgcccg ccgtggggct gctcttcagc
     4261 agcttggcgt gcgcgagcgc gggagatggc gcgtacaacc acacccaaag tagtgccatg
     4321 accagtgccg ggaagagcca ccgcctggct ccgcccacag ttgctgcttt caagaaatgc
     4381 tagagtcctt ctagttccga aacgcgaccg gtgatgaatt acgaattacc aattacgagt
     4441 tacgagttag ggctaactcg taagcgtata cctttaaccc agtacggttt gcgtttcaga
     4501 ctagggtccg tgccaggtca ggccgccgtg gggcgcgccg tcgtggattt tctggggctt
     4561 gccggtcggg ttgccgtcgg ggccgagttg taacttatag aggccgctca cgcccatgaa
     4621 agcgagcgtg ttgttgtcga tccaggcagc gtgaggctgg tcctcggcca tgttggtcag
     4681 gcgcaccgcc ttgccgccgc cgttgcctgg caccatgtag aggtcccacg ggaggccgtg
     4741 cgcgtatgcc acctccggct cgaacgtgag ccacttcaat atgtctaagg ccccgccgcc
     4801 gggcaccgcg ggcgggactt caatagcagc gaaggcgacc ttgctgccat cgggcgagct
     4861 agcgggaccg tagaatgcgg tataagccga gctgtccacc agcacctgcg agagcgaacc
     4921 atcgggcttc tcccgcacga gctttacgcc cggggccgcg cccgcgtccg tgctaacggc
     4981 gtcgggggtg ccctggtaca tgagcgcgcc gtcggtgcca ccttcgaccg ggttgtggga
     5041 gttgtctttc cagtgcgtgc gctcgcccgc tgccaggtcg taccggtcga tgccccgcag
     5101 gccgatgggc atgccgtttg cgtcgtagtt ggtcgaggcc atgtcctgct tctcggcggt
     5161 gaagtacagg tacttgccgt ccgccgagaa ggtagggtcg tagagggtct cgccctcctg
     5221 gtgctgcacg actatttgcg cgcccgtgcc gtcggcgttg atgacccaga tgtcgctggt
     5281 cggtagctcg gtcgggtccg atttgggtgt gtgggcgaag gctatcagct tgccgtccgg
     5341 cgaccacgtc gcatcgttgg cgtactcgct ctcaaccggg aagaaggtga gcctgcgctg
     5401 cttcacagtg tcggtggaca gtatccagag gttcgcgtcc ttcgtgaaga cgtagccgct
     5461 cgacaggggc gggccgttct ccgcgagtgc ctccagcacg gtattgctga ggtcgtaaga
     5521 ctgtggagtg ggagccggcc cgcccgttgc gcggtagtta atctggaccc ggcctgtgta
     5581 cttgcccggc ttgcttggca tggaagtgtt gtaggcccac actccaagct catacgtggc
     5641 ttgcagcacg aacggctcgg cgggcgtgcc cccgtactcg gcaggcttcc ccgcctcatc
     5701 gaagagctgc acgctggccg tacttatcat gctgtcttca atgccgttgt agggcacgcg
     5761 cagtgtgata cgcagcggcg aattgacggt accgagcctc gaatccttgc cctgttcggt
     5821 ggtatattcg agcgtaacag ggccgtcttc gttgccattg ccattctgga cgccggtccc
     5881 ggcttgagac tgggtgacta tctggtaggt gaaaatagcg gctgcgagca ccatgagcag
     5941 ggcgccgatt ggcacgagca cgcgcattgg actggcggac gtttgagttg ggcgggccaa
     6001 aaagaagcaa cctcccagag gtggaactgt gggcaagggg gcaggggtcg tctcttaatt
     6061 atagcaccgt gcgcccctac acgccaaatg cgtgggatga aaacgcatag cataccctat
     6121 acgcagccga agggacgcaa agaggcaaag atgcgtagtt gttgataatt ggcatatatg
     6181 ttgctccgca ctaaggcagc aacttaacga aggaggcttg cgagatggga caccagcacg
     6241 acgatgagaa cgttcacgac atggggcagg ggcactcagg caagggtgac aagggacacg
     6301 cgggcgacaa ggaccacgag gcggggggcg gcaggcgcga gcacagggcc agcggcagcc
     6361 acgaaagtaa catcaccgac accaagaacg actcttccag cagcacgagc ggcgggggca
     6421 gcagcggcgg tggaggcggc agccacggtg gtggcaccaa agagaaccaa taggcgacca
     6481 gccttattct ctaagtggcg gctaaattta aacgccaaga cgccatggta ttagcgtttg
     6541 gaggatacaa agaggaccag ggtacatgat gtagtactct ggtcttcttt gctcttaaac
     6601 tcatttcaaa gccctgttca attctgtaga atgacgttgg ccaaactgaa actctcaagt
     6661 cagtcgtgtg ggatagcctt ggaaaggagt gtaatatctg gatgccaatc atcacgcgag
     6721 aaagtcccaa cacagccgac gctattgccc tcatcaatga gttggaagcg
//
LOCUS       DASRWZ010000557         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_251148, whole genome shotgun sequence.
ACCESSION   DASRWZ010000557 DASRWZ010000000
VERSION     DASRWZ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251148"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /gene="guaA"
                     /locus_tag="VFH60_08775"
     CDS             <1..929
                     /gene="guaA"
                     /locus_tag="VFH60_08775"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET6313915.1"
                     /translation="IDQIREAVGEGRVLCGLSGGVDSSVAAALVQRAIGDRLVCLFVD
                     NGLLRQNEAAEVVETFGRNMHMNLVAVDAADRFLDRLEGVDDPEEKRKRIGETFIRVF
                     EAEALKLGQFDFLAQGTVYPDVIESASPERPSAARIKTHHNVGGLPKDLRFKLVEPLR
                     YLFKDEVREVGLQLGLPEEIVWRHPFPGPGLAVRLLGPITRERLATLRAADAILRHEL
                     READLMRETWQAFAVLLPVRSVGVMGDNRTYADVVAIRAVSSVDGMTADWAKLPYDVL
                     GRVSSRIVNEVAHVNRVVYDITSKPPATIEWE"
     gene            920..2227
                     /locus_tag="VFH60_08780"
     CDS             920..2227
                     /locus_tag="VFH60_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313916.1"
                     /translation="MGISASQTTTQPTRATTQGGATSMPWYRAAVHRLVASEWGTAAL
                     ILAVTRIFALVGGLMGAGRLVQEHPEYAKGWFAEMALMWDAAHYAIIARDGYAFDPAA
                     PAGANVAFAPLFPFLLDLLSGALRWITFGWNFGNEEYGAIIVAGLLISNASFFVALGL
                     LIKWLRPRLGHAGAALVALALASLPTAFFFAAMYTESLFLALVLGAFTLSRSDMRWKW
                     LCVGLLCMLATLTRFAGIILLPVFVVDYLAQTGWRWRTVRSDVLWLGLVPAGVLLYLG
                     FLWWRFGDPFAMNKSMLEGWRHTTSFFLETYWTSLVQLWNSLSGTLPPGDDPVLRYGQ
                     GSRLYIILDLALPVMLLVGGFLARRKLFASEWTWLVLGIIYPLSFNITFSMARYVLPL
                     WPGLVWVGTLRGRARWAAAAAIVPCLLLMAWCTAKYASARWIG"
     gene            2311..3711
                     /gene="purD"
                     /locus_tag="VFH60_08785"
     CDS             2311..3711
                     /gene="purD"
                     /locus_tag="VFH60_08785"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET6313917.1"
                     /translation="MRVLVVGSGAREHAIVWKLATSPGVTDIYCAPGNAGTGVLAQNV
                     DLQIGTESQCDILAGWAFDNRIDLVIVGPEVPLSFGIADTLLLLGVPVLGPTQASARI
                     ETSKAWARDFMARHGIPAPQYRVVQGLDAISAALQDPDTKYPLVLKADGLAGGKGAAI
                     VRDALEAGEAITQMQGTGALPADDAAKKVVIEEYLEGFEVSALAFTDGERVEMMPPSC
                     DYKRLLDGDVGPMTGGMGAYTPTSRVTPELWAQVEAEVLRKAVAGLASEGITYRGVLY
                     AGLMLTDEGPKVLEFNCRLGDPEAQVLIPTLKTPLEDIGVAIAAGDLPRVGPIEWVDE
                     AVVGVVIASENYPTGKPSPVPIGGLESVEEGTLVFHGGTEGAGTLALQPPVGGTTKRK
                     PIFSTLFAGPSQPVAIPDDFDIKATGGRVLTVVGRGPTLDAARAAAYRNVTRIQIAGA
                     QYRTDIALREVEAGEA"
     gene            complement(3948..>4519)
                     /locus_tag="VFH60_08790"
     CDS             complement(3948..>4519)
                     /locus_tag="VFH60_08790"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6313918.1"
                     /translation="FQSLQRRLVMPIEPEVGLSKETVYDGKLFKVVKEEVRLHDGRER
                     PREIVVHPGAVALVVVDDEDRLVLVRQYRRAANAVLLEIPAGTREPGEDAETCARREV
                     LEETGYSAGRVERLGGFYSAPGFCTEFLECFLLTGLSEGQSGGDEDENIEIERLTFDE
                     ALESIRRGEIRDAKSIAGILMWHNLKRAT"
BASE COUNT          761 a         1343 c         1566 g          849 t
ORIGIN      
        1 cgatagacca gatacgcgag gccgtgggcg aggggcgggt gctgtgcggc ctgagcggcg
       61 gcgtcgattc gtcggtggcg gcggcgctgg tgcagcgggc gattggcgac cggctggtgt
      121 gcctgttcgt ggacaacggc cttttgcggc agaacgaagc ggcggaggtg gtggagactt
      181 ttggccgcaa catgcacatg aacctcgtgg ccgtggatgc cgccgacagg ttcctcgacc
      241 ggctggaggg ggtagacgac ccggaggaga agcgcaagcg cataggagag actttcatac
      301 gggtgttcga ggcggaggcc ctgaagctag ggcagttcga tttcctcgca cagggcacgg
      361 tctacccgga cgtgattgag agcgccagcc cggaacggcc ctcggcggcc cgcatcaaga
      421 cgcaccacaa cgtggggggc ttgcccaagg acctgcgctt caagctggtg gagccgctac
      481 gctatctgtt caaggacgag gtacgggagg tcggcttgca gcttgggctg ccggaagaga
      541 tagtctggcg gcaccccttc cccggcccag ggctggcggt gcgcttgctc gggcctatca
      601 cgcgggagcg gctggctacg ctgcgggccg ccgacgccat cctccgccac gaattgcgcg
      661 aggccgacct gatgcgggag acctggcagg ctttcgctgt gctcctccct gtgcgtagcg
      721 tgggcgtgat gggtgacaac cgcacctacg ccgacgtggt ggcgattcgg gcggtaagct
      781 cggttgacgg gatgacggcg gactgggcca agctgcctta cgacgtactg ggccgcgtgt
      841 cgagccgcat cgtcaacgag gtggcgcacg tcaaccgcgt agtctacgat attacctcca
      901 agccgcccgc cactatcgaa tgggaataag cgcgagccag accactaccc agccaacacg
      961 cgcgactaca cagggcgggg cgacctctat gccgtggtac agggcagcgg tgcaccggct
     1021 ggtcgccagc gaatggggca ctgccgcgct catcctggcc gtcacccgca tcttcgcatt
     1081 ggtgggcggg cttatggggg ccggtcgcct ggtgcaagag caccccgaat atgcaaaggg
     1141 ctggttcgct gagatggccc tgatgtggga cgcggcgcac tacgcgatca tagccaggga
     1201 cggttacgca ttcgaccccg cagcacctgc cggcgcaaac gtggccttcg caccgctctt
     1261 tcccttcctg ctagacctgc tgtccggcgc gctccggtgg atcaccttcg gctggaactt
     1321 tggcaacgag gagtacgggg cgatcatcgt ggcggggctg ctcatcagca atgccagctt
     1381 cttcgtggcg ctgggactgc ttatcaagtg gctcaggccg aggttggggc acgcgggcgc
     1441 ggcgttggtg gccctggcgc tggcctcgct gccgacggcc ttcttcttcg cggcgatgta
     1501 caccgagagc cttttcctgg cgcttgtgct tggggcgttc acgctgtccc gctccgacat
     1561 gcggtggaag tggctgtgcg tcggcctctt gtgcatgctt gcgaccctta cccgtttcgc
     1621 gggcatcatt ttgctcccgg tgttcgtggt agactacctg gcgcagaccg gctggcggtg
     1681 gcgcacggtg cgctcggacg tgctgtggct gggattggtg cccgccgggg tgctgctgta
     1741 cctggggttc ctctggtggc gttttggcga ccccttcgca atgaacaaga gcatgctgga
     1801 gggttggcgg catactacgt ctttcttcct ggagacctac tggaccagtc tcgtacaact
     1861 ctggaacagc ctgagcggaa cgttgccccc aggcgatgac ccggtgctca ggtacggcca
     1921 gggcagccgc ctttacatca tcctggacct ggcgctgccg gtcatgctgc tcgtgggggg
     1981 gtttcttgcg cggcgcaagc ttttcgcctc cgaatggacc tggctcgtgc ttggtatcat
     2041 ctacccgctt agcttcaata tcaccttctc tatggcgcgc tacgtgcttc cgctgtggcc
     2101 ggggctggtg tgggtgggca ccttgcgggg gagggcgcgg tgggctgcgg cggctgcaat
     2161 cgtgccgtgt ttgctgctca tggcctggtg cacggcaaag tacgcgagcg caaggtggat
     2221 tggctaagtc acatgccccc ctggacccgg accatagaag aactatcatg tcggattgtc
     2281 gcaacgtaac gtaaggaagg cacatagctg atgagagtac ttgtggtcgg ctccggcgcg
     2341 agagagcatg ccatcgtgtg gaagctggcg accagccccg gcgttaccga tatttactgt
     2401 gcgccgggta acgcgggtac gggcgtgttg gcgcagaatg tcgatttgca gatcggcacc
     2461 gagtcgcagt gcgacatcct ggcggggtgg gccttcgaca accgtatcga cctggtgatt
     2521 gtcgggccgg aggtgcctct cagctttggc attgccgata cgctattgct gctgggcgtg
     2581 ccggtgctcg gcccaacgca ggcgtcggcg cgcatcgaaa cgagcaaggc gtgggcgcgt
     2641 gacttcatgg cgcggcatgg cattcccgcc ccgcagtacc gggtggtgca ggggcttgac
     2701 gccatcagcg cggccttgca agaccccgac accaagtacc cactggtgct caaggccgac
     2761 gggctggccg ggggcaaggg cgcggctatc gtgcgcgacg ccttggaggc aggcgaggcc
     2821 attacccaga tgcagggcac gggcgcgctg cccgcggatg acgcagccaa gaaggtcgtc
     2881 atcgaggagt accttgaggg gttcgaggtg agcgcgctgg ctttcacgga cggggagagg
     2941 gtggagatga tgccgccttc ttgcgactac aagcgcctgc tcgacggaga cgtaggcccc
     3001 atgacgggcg ggatgggcgc ttatacgcca acctcccgcg tcacacctga gctgtgggcg
     3061 caggtagaag cggaggtgtt gcggaaggcc gtggcggggc ttgcaagcga aggcataacg
     3121 taccgggggg tgctctacgc cgggctgatg cttacggacg aggggccaaa ggtgcttgag
     3181 ttcaactgcc gcctgggcga cccggaagcg caggtgctaa taccaacgct gaagacgccc
     3241 cttgaggaca ttggggttgc catcgcggca ggcgacctcc cgcgggtggg gccaatcgaa
     3301 tgggtggacg aggccgtggt gggcgtggtg atagcctcgg agaactaccc gacgggcaag
     3361 ccctcgccgg tgcctattgg cgggctggag agcgtggaag agggcacgct ggtcttccac
     3421 ggtggcaccg agggggcggg cacgctggcg ctacaaccgc cggtgggcgg cacgacgaag
     3481 cgcaagccga tattcagcac cctcttcgcg gggccatctc agccggtcgc catcccggac
     3541 gactttgaca tcaaagccac aggagggcgt gtcttgacgg tagtgggtcg cggtcccacg
     3601 ctcgatgcgg ccagggctgc cgcgtaccgc aacgtgacgc gcatacagat agccggggcg
     3661 cagtaccgga ccgacatcgc gttgagggaa gtcgaggccg gggaggcgta agcggctgcg
     3721 ttacgggttt gtatgacaaa gtgttggaac ggggcaacag gagggggctt tgtcattctg
     3781 agcgcagcga agaatctaaa gaaggtgggt aaggatgccg ttgaacattt cgcctgatgg
     3841 tgctgacgtt caacatagag ccgagggcaa catccaggat tcttcgctac gctcagaatg
     3901 acagaggggg gcttggtacc tcagatgtag cccctctcat aaaccctcta tgttgctcgc
     3961 tttaagttat gccacataag gatgccagcg atggacttgg cgtcgcggat ctcgccacga
     4021 cggatgcttt cgagggcttc atcaaaggtg aggcgctcga tctcgatgtt ctcgtcctcg
     4081 tcgccgcccg actgcccttc gctgaggccg gtgagcagga agcactccag gaactccgtg
     4141 cagaagccgg gcgcggagta gaaacctccg agtcgctcca ctcgacccgc gctgtagccg
     4201 gtctcctcaa gcacctcacg gcgggcgcag gtctcggcgt cctcacccgg ctcgcgcgtg
     4261 cctgcgggta tttcgagcag cacggcgttg gccgcccgcc tgtactggcg caccagcacc
     4321 agccggtcct cgtcgtccac gaccaccagg gccaccgccc cagggtgcac cacgatctcg
     4381 cgcggccgct ctcgcccatc gtgcagccgc acctcctcct tgaccacctt gaatagctta
     4441 ccgtcgtata ccgtttcctt cgacaagcct acttccggct cgattggcat gactagtctc
     4501 ctttggagac tttggaaat
//
LOCUS       DASRWZ010000558         4626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_594686, whole genome shotgun sequence.
ACCESSION   DASRWZ010000558 DASRWZ010000000
VERSION     DASRWZ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594686"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..593
                     /locus_tag="VFH60_08795"
     CDS             84..593
                     /locus_tag="VFH60_08795"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6313919.1"
                     /translation="MAVEADEDKTEQALQDAWPNATCYGCGPANPHGLRIKSYWSEDG
                     SEVICTFEPKPEYNAGFPNVMYGGLVASLIDCHSIWAAIAATYRAEGREHGSLPAISY
                     VTGTLNVRYLKPTPLDRPVTLRARVTDIGPRKAQVACDMYSGEEKTAEGSVLAVRFVM
                     DKSVGAATG"
     gene            913..2694
                     /locus_tag="VFH60_08800"
     CDS             913..2694
                     /locus_tag="VFH60_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313920.1"
                     /translation="MMNPSPGRHAIIVSLAPTMLILKPNMEQVELTLDVRNAGDVVDQ
                     YRVEIDGIEESWYRITVPKVSLFPGDSAQIPILINAPQGAKLTAGQYTFEVRVISQDS
                     PTTIVSANGVILVEAMSDFQVEMEPRRVLGRQGKFRVTVMNLGNSSMQVALSGRDPEE
                     GLQYIFQDPEPELEAGRSKLVPLEVKARGLHFVGSNRSYPFTVSASAVDGPTGQDGRA
                     MTRVTQAEFVRTPILRSLRPLLFLLLLLLLILLIFFIRPDLCKTPLLGNVFCPAPPAA
                     PLPTIESITVDPPSISPGEAVTLSWITTNAITAEVKLGAELISTQPIDETGQGQQTIT
                     GVDRSRAVTLCVYNVEAKRQCSNSQVVVLPTAVVAGNPTPPGGDKASQDQTATAAAGG
                     AGGNGGGNGGNGGAGGPQAQTATAAARANQGAARTATRTATRVPPRATATATRVPPTR
                     APATATRPVATRPPATATLVQPNLTATTIAAATATAAAGGACNPITSPDGEAVPNTAR
                     PGDTILFTARGFEPNEPASFFLTRPDGVVGGTDNPVEGGVDANGVIGPIEFPLAAGFS
                     RFPGRWALTFVGANSNRTVIIVFCVNP"
     gene            2729..3001
                     /locus_tag="VFH60_08805"
     CDS             2729..3001
                     /locus_tag="VFH60_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET6313921.1"
                     /translation="MSRIKISPEQVRQLSGQFKQASQESQQIFNNLSSSVNNIQGEWE
                     GVTQQRFFQEYEQWKTSMSQFVQLLDSIGAQLDTVAARFENVDSGG"
     gene            3113..>4626
                     /locus_tag="VFH60_08810"
     CDS             3113..>4626
                     /locus_tag="VFH60_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313922.1"
                     /translation="MPRIIVNPEQLRQLSTQLLQIAEQLRNIDGRLTNSYNNIEFEVQ
                     ERGQIDGEVMQARSRANELIGLSDSLARRITGKVQAFEEADREGAAGLPAAPPPMKLD
                     GAGADPNAGGQQAGVQGAGSPGGSTVIVDQSSAGAASADGASDAGVADSIAGGPSDQA
                     VPGAEAASADGAAAPVEQPAEQPAAQSGDGAQQGAEVPPAGQGAPDASAGAGGQGDAG
                     AQGNQAEAAASSASQHAFAKPLQNVADDLLNEPPADGGAGGADGGVGAAAGVGGGDAG
                     GAVVADAGGGGGAEPAPDTGQPSTDSQPPVADAQTEPPPPGPVDSGEPQPEPEPQREP
                     PPEPRPEPEPEPQPEPEPAPEPQPEPEPQPEPEPEPEPQPEPEPEPEPQPEPAPIPPG
                     VGEPAPEPPPAEPPPPAEPPPAEPPPVEPPPAEPPPPVEPPPAEPPPVEPPPVEPPPA
                     EPPPVEPPPAEPPPVEPPPVEPPPVEPPPVEPPPAEPPPVEPPPVEPPPVEPPPA"
BASE COUNT          953 a         1545 c         1405 g          723 t
ORIGIN      
        1 tttgccagca ggcatcacct cttcgctccg ttatgctata ataccccccg ttataccgcc
       61 aggatacgtg acgggaggta tggatggcag tggaggcaga cgaagataag accgagcagg
      121 cactacagga cgcctggccc aacgccacgt gctacggctg cggcccggcc aacccgcacg
      181 gcctgcgcat caagagctac tggtcagaag acggctcaga ggtcatatgc acatttgagc
      241 cgaagcccga atacaacgcg ggcttcccca acgtcatgta cggtgggctg gtcgcgtcgc
      301 ttatcgactg ccactccata tgggctgcta tcgccgccac ctaccgggca gaggggcgcg
      361 aacacggctc gctcccggcc atcagctacg tgaccggcac cctcaacgtg cgctacctga
      421 agcccacccc gctcgacagg ccggtaactt tgcgagccag ggtgacggac atcgggccgc
      481 gcaaagccca ggtcgcctgc gacatgtatt cgggcgagga gaagaccgcc gaggggagcg
      541 tgctggccgt gcgcttcgtg atggataaat cggtaggcgc ggctaccggg taaccaaagc
      601 gccatcgttc cctgcgcgaa ttgcatttcc gaatctattg tcatgcgcta aagtgtgtga
      661 tatactttgg tacgctaccg cctgagactt caaaggctct cgtttgccct gctttaagtc
      721 caacatgcat atcgcgcttc ctgggtgagc tacgttcctc tctggcttgg gacgctgccc
      781 actaccaggt agatttttct caagcagagg ttattccctg tacccgttct atacccctga
      841 ccatcgccgc gccttgagag ccaaccgggt ctctggcagg gcgcaaatat ctatgtacag
      901 agagatattg ccatgatgaa cccctcgccc ggcagacacg ccataatcgt cagcctcgcg
      961 cccaccatgc tcattctcaa gccgaacatg gagcaggtcg agcttaccct cgacgtgcgt
     1021 aacgccgggg acgtggtaga ccagtacagg gtggaaatag atggcattga agagtcctgg
     1081 tacaggatca ccgtccccaa agtctccctc ttccccggcg acagcgcaca gatacccatc
     1141 ctcataaacg ccccccaggg ggccaagctg accgccgggc agtacacctt tgaagtgcgc
     1201 gtcatctccc aggactcccc taccactatc gtatccgcca acggcgtgat cctggtggag
     1261 gcaatgtctg acttccaggt tgagatggag cctcgaaggg tgcttggacg ccagggcaag
     1321 ttccgggtga cggtgatgaa cctgggcaac agttcgatgc aggttgccct gtcggggcgc
     1381 gacccggagg aggggctgca atacatcttc caagaccccg aacctgaatt ggaagcaggc
     1441 aggtctaaac tggtgccgct agaggtaaaa gcgcgcggcc tccatttcgt ggggtctaac
     1501 cgctcttatc ccttcacggt gagcgcgagc gccgttgacg gcccaaccgg gcaggacggt
     1561 agggcgatga cgcgcgttac ccaggccgag ttcgtgcgca cgcccatact gaggtcgcta
     1621 cggccactgc tatttctgtt gctgctcctg ctcctcatac tgctcatctt cttcatacgc
     1681 cccgatttgt gcaagacgcc gctcctgggc aatgttttct gcccggcacc ccccgcggcc
     1741 ccactaccga ccatagagtc gataacggtt gatcccccct ccatctctcc cggcgaggcc
     1801 gtcaccttga gttggataac cactaacgcg atcacagccg aggtgaaact gggagcggag
     1861 ctgatctcta ctcagcccat tgacgagacg gggcagggcc agcaaacgat tacgggcgtt
     1921 gacaggagcc gcgcggttac gctgtgcgtc tacaacgtcg aggcaaagcg ccaatgcagc
     1981 aatagccagg tggtcgttct gcccaccgcc gtagtcgcgg gcaaccccac gccgcccgga
     2041 ggcgacaagg ccagccagga ccagaccgcc acagccgccg ccggtggtgc aggcggcaac
     2101 ggtggaggca atggcggtaa tggcggggca ggcggaccac aggcgcagac cgccaccgcc
     2161 gcggcgcggg caaaccaggg cgcggcgagg accgctacca ggacggctac cagggtaccg
     2221 ccgcgagcca cagccacagc cacacgagtc ccgccgacgc gcgcgcccgc gactgccaca
     2281 cgccccgtag ccacgcgccc acccgcgacg gctacgctgg tgcaacccaa cctgaccgct
     2341 accacgatcg ctgcggccac cgccactgct gccgcaggcg gagcgtgcaa tccgataacg
     2401 agtcccgatg gcgaggccgt ccccaacacc gccaggccgg gcgacaccat attgttcacg
     2461 gccaggggct tcgagcctaa cgagcctgcc tcgttcttcc ttaccaggcc ggatggggtg
     2521 gtaggcggca ccgataaccc tgttgaaggc ggcgtagatg ccaacggcgt catcggcccg
     2581 atagaattcc cattggcagc aggtttcagc cggttcccag gacgctgggc gctcaccttc
     2641 gtgggcgcga acagcaaccg cacggttatt atcgtcttct gtgtcaatcc ctagagcgac
     2701 gaatcaacca taggaggtaa gaaaattcat gtcccgcatc aagatttccc ccgaacaggt
     2761 acgccaactg tcgggccagt tcaagcaggc cagccaggag agccagcaga ttttcaacaa
     2821 tctctcgtcc agcgtcaata acatacaggg cgaatgggaa ggcgtgaccc agcagcgatt
     2881 cttccaggag tacgagcagt ggaagacctc catgagccag ttcgtgcagc ttctcgatag
     2941 catcggcgcg cagctcgaca ccgtagccgc ccgcttcgag aacgtagact ccggcggttg
     3001 atacgggccg ataggaactt cgtcccggcg aggctgaggc gtgcgcctct cgccggtaac
     3061 gcaacccaaa tagaggcagc aatcgttatc tgtacgccgt ggaggggtat ttatgccacg
     3121 cattatcgtg aatcccgaac aactgcgcca gttgagcacg cagttgttgc agatcgccga
     3181 gcagttgcgc aatatagatg ggcggctgac caacagttac aacaacatag agtttgaagt
     3241 acaggagagg ggccagatag acggcgaggt gatgcaggcc cgcagccgcg cgaacgaatt
     3301 gataggcctg tctgactcgc tggcacgccg catcaccggc aaagtgcagg cttttgagga
     3361 agcagaccgc gagggcgcgg caggcctccc agccgcccca ccccccatga agctggacgg
     3421 cgcgggcgcg gacccgaacg ccggagggca gcaggcgggc gtccagggtg ctggatcacc
     3481 cggaggctcg actgtaatcg tagaccagtc ctcggcagga gcagcctccg ccgatggagc
     3541 atctgacgcc ggggtagccg actccatagc cggaggccca tccgaccagg ccgtgccggg
     3601 agcagaagca gcatccgccg acggggcagc tgccccagtc gagcaaccag ccgaacagcc
     3661 tgccgctcag tctggggatg gtgctcaaca aggcgcggaa gtgcctcctg ccggccaggg
     3721 agcaccggac gcgtctgccg gggccggtgg tcaaggcgac gcgggtgcgc aggggaatca
     3781 ggctgaagcg gcggctagct cggccagcca gcacgctttc gccaagccac tgcaaaatgt
     3841 agccgacgac ttgctgaacg agcctccggc agacggagga gcaggcggcg cagatggcgg
     3901 cgtgggtgcc gccgcgggcg tgggtggtgg cgatgcaggc ggtgcggtgg tagcggatgc
     3961 cggtggtggc ggtggggccg aaccagcccc cgacaccggc cagccctcaa ccgattctca
     4021 gccgccggta gccgatgccc aaacggaacc tcctcctccg ggacccgtag attcgggcga
     4081 acctcagcca gagccagagc cacagcgtga gcctcctcct gagccacgac ccgaaccaga
     4141 acctgaaccg cagccggagc cggagccagc acctgaaccg cagccggaac cggaacccca
     4201 acctgagcca gagccagagc cagagccgca acccgaacca gaaccggaac ctgagcctca
     4261 gccagagcct gcgccgatac cccctggggt tggcgagcct gcgccggaac cgccaccggc
     4321 agagccacca cctcccgcag aaccgccgcc tgctgagccg ccaccagtgg agccgccacc
     4381 ggcagagcca ccacctccgg ttgagccgcc cccggcggaa ccaccgccag tggaaccgcc
     4441 gccagtggaa ccgccgcctg ctgagccacc gccagtggaa ccacctccgg ctgagccacc
     4501 cccggttgag ccacccccgg ttgagccacc cccggtggaa ccgcctccgg tcgaaccgcc
     4561 gcctgctgag cctcctccgg ttgagcctcc tccggttgag ccacccccgg tggaaccacc
     4621 acctgc
//
LOCUS       DASRWZ010000559         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1142798, whole genome shotgun sequence.
ACCESSION   DASRWZ010000559 DASRWZ010000000
VERSION     DASRWZ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142798"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..>2009
                     /locus_tag="VFH60_08815"
     CDS             163..>2009
                     /locus_tag="VFH60_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HET6313923.1"
                     /translation="MNDNLYLLPHPRSLSLSAGRYALTPNGRILLEGAAPSELLGAGR
                     RLQAALAKHADLTWELSAGGLGPVDEVGIVLRLAPGGVAHREGYELTITPEGTVIQAQ
                     SPVGVFYGVCTLVQILQQRGRNLPALHVTDWPDFAARGLMLDISRDKVPRMETLFELI
                     DMLASWKLNQLQLYTEHTFAYRRHPDVWVKASPITGDEVLALDLFCRERHIELVPNQN
                     SFGHMQRWLAHDRYAALAEVHGEFQVPWGTMQGPFSLCPGDPGSLELVRGLYDELLPH
                     FSSKQFNVGCDETFDLGQGRSREECERRGVERVYLDYLIKIHREVEERGYTMQFWGDI
                     IVHTPELISELPKNSIALEWGYEADHPFDEHGAMFANSGIPFYVCPGTSSWTSIAGRT
                     DNAVGNLLSAAENGLKHGAAGYLNTDWGDHGHWQTLPISYLGFAVGAAYSWALEANRD
                     MDVAAVVSTHAFHDPTGSMGRVAYDLGNVYKAPEVSVHNGSALFWTLQRPFEQLRKSE
                     RPRPDFASVLEAIDAAMTPLSKARIHRPDAALIVRECENTASLLRHACLRGRLAAQPG
                     DAEDATSKALDEDMRDIIREYEHIWLERNRPGGLADSIVALEKARADYKT"
BASE COUNT          408 a          653 c          605 g          343 t
ORIGIN      
        1 cccacagggg aatccactac ccctccctat cagtaactac accctgggcc agtgccacaa
       61 caagattgac agcactacca actggtgcta gactgcctcc caaaccgtaa taatctgcgg
      121 ccgtatcagg ctgcttcaag gaggttgcca cgccaggcaa acatgaacga taacctgtac
      181 cttcttccgc acccacgctc cctttcactc tccgctggac gttacgcgct taccccgaac
      241 ggccgcatcc tgttggaagg cgcggcacct tcggaattgc tgggggcggg caggcgtctg
      301 caagcggccc tcgcaaagca cgcggacctg acatgggagc tttctgccgg tgggttaggg
      361 ccggtggatg aggtcggtat cgtgctgcgc ctggcccccg gcggggtcgc ccaccgagag
      421 ggctacgaac tgactatcac accggagggg accgtcatcc aggcacaaag cccggtgggg
      481 gtcttttacg gggtgtgcac cctggtccaa atcctccagc agcgcgggcg caacctgcct
      541 gccctgcacg ttaccgactg gcctgatttc gcggcgcgcg gcctgatgct tgatatcagc
      601 cgagataagg tgccccgcat ggagacgctc ttcgagctta tcgatatgct cgcaagctgg
      661 aaactgaacc agcttcagtt gtacaccgag cataccttcg cttaccgccg gcaccccgac
      721 gtttgggtca aagcctcacc tatcacgggc gatgaagtcc tcgcgctcga tctgttctgc
      781 cgggagcgac acatagaact ggtgcccaac cagaactcct tcggccacat gcagcgctgg
      841 ctggcccacg accgctacgc cgcgctggcc gaagtgcacg gggagttcca ggtgccgtgg
      901 ggcacgatgc aggggccgtt tagcctgtgt ccgggcgatc ccggcagcct ggagcttgtg
      961 cgcggcctct acgacgaatt gctgccccat ttctccagca agcagttcaa cgtaggctgc
     1021 gacgagacat ttgacctggg gcagggccgc agccgggagg agtgcgagcg acggggtgtg
     1081 gagcgcgtgt acctcgacta cctgatcaag atacaccggg aagtcgagga gcggggttac
     1141 acgatgcagt tctggggcga catcatcgtg cacactcccg aactgatctc cgagttgccg
     1201 aaaaacagca tcgcgcttga gtggggttac gaggcagacc acccgtttga cgaacatgga
     1261 gccatgttcg caaactccgg cataccattc tatgtctgtc ccggcacctc ctcctggacc
     1321 tccatcgccg ggcgcaccga caacgccgtg ggcaacctgc tgagcgccgc cgagaacggg
     1381 ctgaagcacg gggcggcagg ctacctcaac accgattggg gcgatcacgg acactggcag
     1441 acgcttccca tcagctacct gggcttcgcg gttggggcag cctattcgtg ggcgctcgag
     1501 gctaaccgtg acatggacgt agccgcagtc gtaagcacgc acgccttcca cgaccccacc
     1561 ggcagcatgg ggcgcgtggc ctacgacctg ggcaacgtct acaaagcgcc cgaagtgagc
     1621 gtgcataacg gctcggcgct cttttggacg ttgcaacgcc ccttcgagca attgcgcaag
     1681 agcgaaagac ccaggccgga cttcgcctca gtgctcgaag ccattgacgc cgcgatgacc
     1741 cctctctcca aagcccgcat acacaggccc gacgcggccc tcatcgtgag agagtgcgag
     1801 aacacggcca gtctgctgcg ccacgcgtgc ttgcgcggac gactggcggc ccaaccgggg
     1861 gatgcagaag acgccacaag caaagccctc gatgaagata tgcgcgacat catccgggag
     1921 tacgagcaca tctggctgga gcgcaaccgc ccaggtggcc tggccgacag catagttgcc
     1981 ctcgaaaagg cccgcgcgga ctacaagac
//
LOCUS       DASRWZ010000560         6215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_662885, whole genome shotgun sequence.
ACCESSION   DASRWZ010000560 DASRWZ010000000
VERSION     DASRWZ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662885"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3078
                     /locus_tag="VFH60_08820"
     CDS             <1..3078
                     /locus_tag="VFH60_08820"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HET6313924.1"
                     /translation="EYWTWQEFDAVENAVASGVNVLFLSANEAYWRIRMESSSLGPNR
                     IITGYKDATLDPIKSGPDGPTVTFRNLGRSENRLLGTGYMSYYDDALYNMPWQPSVDA
                     SKWYWDCTGLRTGDSVNNIVGEEWNAILYNYNSVTPQGIELLSEGVATSDRGVPYPQN
                     TTIYTAPSGAMVFAAGSIHWSWGLMDHSYRNQVFQSYAVSNDADARIQQLTANILDRF
                     AGYWDGTPRGCTGQEFYKTGPRPTRTPMPVPPTATGTLPTLPPTRTSTPTSQTGGSTA
                     TPAGTATNTSIPANTGTATRTATPFPTNTATQTPVPGGACTVTLAATDLPKDIRDLST
                     TTSAISVSGAGTVADLAVLGVSIEHTYASDVKAVLVSPQGTRVELFAAVCGAGAWTAA
                     NTGFGISQAGAGAMGSVCPPGQGTYRPEEASLAPFVGQQAQGTWTLEVSDGGQYDVGK
                     LHGWQLKITLNGASCGSVPSATSTATNTQQPTSTNTSIPTATGTATNTRTATATATAT
                     ATDTQQPTNTAIPTSPATQTPMPTNTATPTSTNTVQPSSTATQTPLPPNTATSTATRT
                     ATSSSTAQPPATGTATRTATRTATATATRTATKTATATRTGTATRTATRTATPVAATP
                     TRTPTSTNTPVRTATQTPGTGGSCSALYTSADVPRAIPDRGTTFSTLMVSDAGNIAQV
                     EVRGLSLAHTYASDTTVFLISPQGARVELFSHRCGSNVWTQANTGFGLKQGASTAIGT
                     TCPPGSGTYIPAGSLSAFAGQQANGTWKLEVNDTGAYDLGTLHAWSLSISYSNPTCPV
                     GTQAHAGTPIPPTSTPNTTFGDVTPQSVFKPHLEWLAARGYISGYACGAEGEPCPGTY
                     FRPSASVTRGQLLKMVVSAAGWAIDNPKDSSFSDVPRDSAFFIYIETAARRGIINGYG
                     CGSDTEPCDEEGRPYFRATSNITRGQLSKVLALALGLPAPAAGTQTFADVPEDSPFHA
                     YVEAMAAGHIVGGYACGSAVEPCDDGHRPYFRPADSASRGQVAKFVAVGYGER"
     gene            3424..4974
                     /locus_tag="VFH60_08825"
     CDS             3424..4974
                     /locus_tag="VFH60_08825"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET6313925.1"
                     /translation="MAQIPDFLTDEKSIVGGIYTALIGTSRARSIAPAIQPQVARANA
                     EANLRTAPLGRRVSGLGLGERKLLQAFTDWVVLAGGCFLVLSFSDRLYSRSAMGVSLM
                     MLTLLWFFFADAFDAYKVPVMQSRFYTTYAVAKVMIVTTISYTVLAWFAGGVLPIIRP
                     RISETLLIAALLVPIATVRVLMSLAMSSAPLRRRVVVVGANQDGFEMVEAIRRYDGKT
                     YEFLGYFDDETDAERDDAPILRERIQPTSELLAVNDMLGIDQVVLANPLQTGPLLHTL
                     SRLHEQGAQITPMFAIYQDLTGRVPVSHLGNDWYVALPANVKNTTRSYQLIKRGMDIV
                     LSLGMLIVAAPILPFVMLAIKLDSPGPIFFKQVRVGLGGKPFKIMKFRSMRQDAEAGS
                     GAVWASNGDPRVTRIGRFLRKSRIDEIPQLWNIVVGDMSFVGPRPERPEFDEQLEREI
                     PFYRARRAVRPGLTGWAQVQYRYGNSIADALRKVEYDLYYIKNESVYLDLLILLRTVA
                     VVLKLGGN"
     gene            5024..5980
                     /locus_tag="VFH60_08830"
     CDS             5024..5980
                     /locus_tag="VFH60_08830"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6313926.1"
                     /translation="MRLTVSAVITTFNYARFIPAAIESVMAQTLPPDEIIVVDDGSTD
                     DTAEVVARYAGRGVRYVYKENEGGSSARNAGIAASKCDLVAFLDADDRWLPHKLERQV
                     AQMVKYPQVGLVSGGEWQVDARGEHPYLVARETLEAAWVFKKMLVENFTGNTSLALVR
                     RSVFDKVGTFDEKVPLGHDWDMWLRIAKDYPISVIGEPLIYYTRHSGSLSAGKIEQRY
                     RSNRQFHRRYIGQLPTLWERAWVLRAAQSMNLYYTAVGMGDDRHRRFSAARMALAALA
                     LDPFYKTKLKLGLLLRSGLGEGAFGRISHGLHARVPHETRGI"
     gene            6026..>6215
                     /locus_tag="VFH60_08835"
     CDS             6026..>6215
                     /locus_tag="VFH60_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313927.1"
                     /translation="MSTILQAEYPHVDEAIHKPGIAASLTPGAIRLSVGEVNSRAEFM
                     LMQDEWNALVRATEDQPFY"
BASE COUNT         1233 a         1965 c         1988 g         1029 t
ORIGIN      
        1 gagtactgga cctggcagga gttcgacgcg gtggagaacg cggtcgctag tggggtgaac
       61 gtgctcttcc tgtcggcgaa cgaagcgtac tggcgtatca ggatggaaag ctccagcctg
      121 ggccccaacc gtattataac gggctacaag gacgcgacgc tcgaccctat caagtcgggg
      181 ccggacgggc cgacggtgac cttccgcaac ctgggccgct cggagaaccg cctgctgggc
      241 acgggctaca tgagctacta cgacgacgcg ctctacaaca tgccctggca gccgagcgtg
      301 gacgcctcca agtggtactg ggactgcacc ggcctgcgca ccggggactc ggtcaacaac
      361 atcgtgggcg aggagtggaa tgccatcctg tataactaca actcggtgac gccgcagggc
      421 atcgagttgc tgtctgaggg agttgcgacg tctgacaggg gagtgccgta cccgcagaac
      481 accaccattt acaccgcacc cagcggggct atggtgttcg cggcgggcag catccactgg
      541 tcgtgggggc tgatggacca ctcgtaccgg aaccaggtat tccagtcgta cgcagtgtcg
      601 aacgacgccg acgcgcgcat ccagcagtta accgcgaaca tcctcgacag gttcgcgggt
      661 tactgggacg gcacgcccag gggctgcacc gggcaggagt tctataagac cggcccccgc
      721 cctacccgca cgccgatgcc ggtgcctccc acggccacgg gcaccctgcc cacgttgccg
      781 cccacccgca ctagcacccc gacctcgcag acagggggca gcactgctac tccagccggg
      841 acggccacca acacgagcat tccagccaat accggcacgg ccacgcgcac ggcaaccccg
      901 ttccccacga atactgctac gcaaacaccg gtcccgggtg gggcctgcac cgtgacgctc
      961 gccgccaccg acttgcccaa ggatattcgc gacctgagca cgaccacctc ggctattagc
     1021 gtgagtgggg cgggcacggt agccgacctt gcggtgcttg gtgtgagtat cgagcatacg
     1081 tatgccagcg acgtgaaagc agtgctcgta agcccgcagg gcacgagggt ggagctattt
     1141 gcggccgtat gcggtgccgg tgcgtggacg gcggcgaata cgggatttgg tatctcgcaa
     1201 gcgggtgccg gcgctatggg gtcggtctgt ccgccggggc agggcaccta caggccggag
     1261 gaggccagtt tagcgccctt tgtggggcag caggcacagg gcacgtggac gttggaggtg
     1321 agcgacgggg gccagtatga cgtgggcaag ctccacggct ggcagttgaa gattacgctg
     1381 aacggcgcga gctgcgggtc agtgccgagc gccacctcga cggcgaccaa cacccagcag
     1441 ccgaccagca ctaatacgag cataccaacc gctacaggga cggctaccaa cacccggact
     1501 gccactgcta ccgctactgc taccgcgacc gatacccaac agccgaccaa taccgctatc
     1561 ccgactagcc cggctactca gacgccgatg ccaacgaata ctgctactcc aacgagcacc
     1621 aacacagttc agccgagcag taccgccacg caaacgccac tgccgcccaa taccgcgact
     1681 tctactgcta ctcgcaccgc gacctccagc agtacggcac agcccccggc taccggcacg
     1741 gcgacgagga ccgctaccag gactgctacc gccacggcca ctcgaacggc tactaagact
     1801 gctaccgcca cacgcacggg caccgcgact cgtacagcta ccaggacggc cacaccggtt
     1861 gccgctacgc ccacccgcac cccgaccagc accaacacgc ccgtgaggac ggccacgcag
     1921 acgccaggca ccgggggaag ctgctcggcc ctatatacga gcgcggacgt tccgagggcg
     1981 atacccgacc ggggcactac cttctcgacg ctgatggtaa gtgatgcggg caatatcgcc
     2041 caggtggagg ttcgtggcct aagccttgct catacctacg cgagcgacac tacggtgttc
     2101 ctcatttcgc cgcagggcgc gcgtgtggag ttgttctccc acagatgcgg cagcaacgtc
     2161 tggacgcagg ccaacacggg cttcgggttg aagcaaggcg cttccacggc tataggtacg
     2221 acgtgccctc ctgggagcgg tacgtatata cccgcgggca gtctcagtgc attcgcgggt
     2281 cagcaggcca acggcacgtg gaagcttgag gtgaacgata caggcgcata cgacctggga
     2341 actttgcatg cctggagctt gtcgatcagc tacagcaatc ccacttgccc ggtcggcacg
     2401 caggcccacg caggcacccc gatacctccc accagcaccc ccaataccac ctttggcgac
     2461 gtgacgccgc agagcgtgtt caagccgcac ctggaatggc tggccgcgag gggctacata
     2521 agcggctacg cgtgcggtgc ggaaggcgag ccgtgtccag gcacctactt ccgaccctct
     2581 gccagcgtga ccagggggca gttgctgaag atggtggtga gcgcggcggg ctgggctatc
     2641 gacaacccca aggactccag cttctcggac gtgccaaggg acagcgcctt ctttatctac
     2701 attgagacgg cagcccgacg cggcatcatc aacgggtatg gatgtggaag tgacacggag
     2761 ccttgcgatg aggagggccg cccgtacttc cgcgcaacta gcaatatcac acgcgggcaa
     2821 ctgagcaagg tgctggcgct ggcgctaggt ctaccggccc ccgcggcggg cacacagacg
     2881 ttcgcggacg tgccggagga cagcccgttc cacgcgtatg tggaggcgat ggcggcgggc
     2941 catatcgtgg gtgggtatgc gtgtggaagc gcggtggagc cttgcgacga cgggcaccgg
     3001 ccctacttcc gcccggccga tagcgcctca cgcgggcagg tcgccaagtt cgtagccgtg
     3061 ggctacggag agcggtaggg accgcttcaa gtaagcacat tagagtcggg ggagccaggt
     3121 tactggggag cctcctccga caatgagtag atgaggcaca ttttaggagt gcaagatata
     3181 ccacgctcgc gccaaaagag gtaccaccat cgggtgattg ccccggatgc gcatcacctt
     3241 acaatggtga tacccagcag tactttatag atttcgaggg cgataaatag cttcgtttta
     3301 cgagctgagc gggtgctctg cttaactacc cccacgccca aaaagggcct gtacaaatag
     3361 gagcggtaac cctgagggcg catcgggggc taacctgtct cgaacgagga agggattatc
     3421 ctaatggcgc agataccaga ttttctaaca gatgagaaga gcatagtagg ggggatttac
     3481 accgccctga taggtacttc ccgcgccagg tccatcgccc cggcaattca gccccaggtc
     3541 gccagggcga acgccgaggc caacctccgc accgccccgc tgggacgcag ggtcagcggc
     3601 ctggggcttg gggagcgcaa gctgttgcag gctttcaccg actgggtggt gctcgccggc
     3661 ggctgcttcc tggtgctgag cttcagcgac cggctttact cccgaagcgc gatgggcgtc
     3721 agcctcatga tgctcaccct gctctggttc ttcttcgcgg acgcttttga cgcctacaaa
     3781 gtgcctgtaa tgcagagccg cttctacacc acctacgcgg tcgccaaggt gatgatagta
     3841 accacgatca gctacacggt gctggcctgg ttcgcgggcg gggtgctgcc catcatccgg
     3901 ccccgtatca gcgagacgct gctgatagcc gcgctcctgg tgccgattgc caccgtgcgc
     3961 gtgcttatga gccttgcgat gtcgagcgcg ccgctgcgca ggcgcgtggt ggtggtgggt
     4021 gccaaccagg acggcttcga gatggtggag gcgatacgcc gctacgacgg caagacctat
     4081 gagttcctgg gctacttcga cgacgaaacg gacgcggagc gcgacgacgc gccgatcctg
     4141 cgcgagcgaa tccagccgac ctccgagctg ctggcggtga acgacatgct cggcatcgac
     4201 caggtggtgc tcgccaaccc gttgcagaca gggccgctcc tgcacacgct gtcccgcctg
     4261 cacgagcagg gcgcgcagat caccccgatg ttcgctatct accaggacct caccgggcgc
     4321 gtgcctgtgt cgcacctcgg caacgactgg tacgtggcgc ttcccgcgaa cgtaaagaac
     4381 accacccgca gctaccagct aatcaagcga ggcatggaca tagtgctgtc gctgggcatg
     4441 ctcatcgtgg ccgcgcccat cctgccgttc gtgatgctgg cgatcaagct cgactcgccg
     4501 gggccgatct tcttcaagca ggtgcgagtg ggcctgggcg gcaagccctt caagatcatg
     4561 aagttccggt cgatgcgcca ggacgccgag gccgggagcg gggcggtgtg ggccagcaac
     4621 ggcgaccccc gtgtgacccg cataggccgc ttcctccgca agagccgcat tgacgagata
     4681 ccccagcttt ggaacatcgt ggtgggcgac atgagcttcg tcggcccccg ccccgaacgc
     4741 cccgagttcg atgagcagtt ggagcgggag ataccgttct acagggcgcg gcgggcggtg
     4801 cggccgggac tcaccggctg ggcgcaggtg cagtaccgct acggcaactc catcgccgat
     4861 gcgctgcgca aagtagagta cgacctgtac tacatcaaga acgagtcggt ttacctcgac
     4921 ctgcttatcc tgctgcgcac cgtggccgtg gtgctcaagc ttggcggcaa ctaagtccta
     4981 ttgaattgca aacgcataac atgacctgca cggtacacga cctatgagac tcacagtcag
     5041 cgcggtaatc accacattca actacgcccg gttcatcccg gccgcgattg agagcgtcat
     5101 ggcgcagacg ctgcctcccg acgagataat agtggtggac gacggctcga cggacgatac
     5161 ggcggaggtt gtggcgcggt acgccgggcg tggcgtgcgc tacgtctaca aggagaacga
     5221 gggcggaagt tcggcgcgca acgcgggcat cgcggccagc aagtgcgacc tggtggcctt
     5281 cctcgacgcg gacgaccgct ggctgcctca caagctggag cggcaggtcg cgcagatggt
     5341 gaagtatccc caggtgggtc ttgtttcggg tggcgagtgg caggtggacg cgcggggcga
     5401 gcacccgtac ctggtagcgc gggagacgct ggaggccgcg tgggtcttca agaagatgct
     5461 ggtggagaac ttcaccggca acacctctct ggcgctggtg cggcgctcgg tgttcgacaa
     5521 ggtgggtaca ttcgatgaga aagtgccact gggccacgat tgggacatgt ggctgcgcat
     5581 cgcgaaggac taccccatca gcgtgatagg ggagccgctc atctattaca cgcggcactc
     5641 cggcagctta tcggcgggca agatagagca gcgttaccgc tccaaccgcc agttccaccg
     5701 ccgctacata gggcaacttc ccaccttgtg ggagcgggcc tgggtgttga gggcggcgca
     5761 gagcatgaac ctgtattaca ccgccgttgg gatgggcgac gacaggcaca ggcgcttctc
     5821 cgccgcccgg atggccctgg ccgcgctggc cctggacccg ttctacaaga caaagctcaa
     5881 gctgggcctg cttctgcggt ccgggcttgg ggagggcgca ttcgggcgca tcagtcatgg
     5941 cctgcacgcc agggtgccac acgagactag aggtatttag cggcccctga gccgtcgcaa
     6001 gcgcgagaca ccgcccggag cacacatgtc aactatctta caagccgaat accctcatgt
     6061 tgatgaggct atacacaagc ctggaattgc cgcgtccctc acaccgggtg ccatcaggct
     6121 gagcgtaggc gaggtgaata gccgcgccga gttcatgctg atgcaggacg agtggaacgc
     6181 gttggtgcgg gctaccgaag accagccctt ctacc
//
LOCUS       DASRWZ010000561         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_389767, whole genome shotgun sequence.
ACCESSION   DASRWZ010000561 DASRWZ010000000
VERSION     DASRWZ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389767"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..515
                     /locus_tag="VFH60_08840"
     CDS             192..515
                     /locus_tag="VFH60_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET6313928.1"
                     /translation="MSVEEKLGLFFGSITVFVLAVFLGVELISKVPTTLHTPLMSGTN
                     AIHGIVLVGAMVVAGLADQSLLVTVLAFVAVIFAAGNVVGGFVVTDRMLQMFKSKRPD
                     AGGKK"
     gene            519..1979
                     /locus_tag="VFH60_08845"
     CDS             519..1979
                     /locus_tag="VFH60_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008275441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET6313929.1"
                     /translation="MSNPVSWLLFQGEEAHRSWLGLTPSTDVYLTIPYIIAAICFIIG
                     IRRLSSPKTARAGNRIAAVGMGVAVVATFFSSSVQNVWFILAGIVIGGGVGFFLARAV
                     QMTEMPQMVALFNAMGGGAAALTSTVEFLEIINRGSTPDLDVAISTVLGVLIGAMSFS
                     GSIVAIAKLYGWISSAPITYGGQQIVNAVLALGAVGLGVWLVIQPNPLALIGLVALAA
                     VLGVLLVLPIGGADMPVVISLLNSFTGMAAAFTGFVLSNPVLIIAGALVGASGMLLTV
                     LMGRAMNRSVTNVIFGAFGSAGVDVGGGASAAGKSVRELGVDDAATLMAYANQVVVVP
                     GYGMAVARAQNAVRELSDLLQQKGVTVKYAIHPVAGRMPGHMNVLLAEADVPYNQLFE
                     MEDINPEFSRTDVALIIGANDVVNPAARNDPKSPIYGMPILDVDKAQNVIVMKRSMRS
                     GFAGIENELFYEDRTSMLFGDAKEMAEALVASVKQL"
     gene            2330..>2655
                     /locus_tag="VFH60_08850"
     CDS             2330..>2655
                     /locus_tag="VFH60_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6313930.1"
                     /translation="MSVLDDVKEALGETVPEVAVEEREAVDARTYDCAIVGGGPAGLS
                     AAIYMGRMRRSVVVIDDDQGRSNWHQVNRNYLGFVDGIHAKSLREVGEKQAGKFGVRF
                     LAARATG"
BASE COUNT          477 a          744 c          929 g          505 t
ORIGIN      
        1 aaggaggccc agttcggccc caactacgag gacgaggtag tggccggtac gtgcatcact
       61 cgcgacggcg agatagtcca ccccggcgtc aagtcggcgg tggaagccaa cctacagagg
      121 agcacagcat gatctcttca ctagctatac ttgccaacac ggtggcggcg caggccaatg
      181 agacgggcgg catgtcggtg gaggaaaagc tcggcctgtt cttcgggagc atcaccgtgt
      241 tcgtgctggc ggtgttcctg ggggtggagc ttatttctaa ggtgccgacc acgttgcaca
      301 ccccgctcat gtcgggcacc aacgccatcc acggcatcgt gcttgtgggc gcgatggtgg
      361 tagcggggct ggcggaccag agcctcttgg tgacggtgct ggccttcgtc gcggtcatct
      421 tcgcggcggg taacgtggtc ggcggcttcg tggtgaccga ccgcatgctg caaatgttca
      481 agtcgaagag acctgacgcc ggaggaaaga agtaaccaat gtctaaccca gtatcctggc
      541 tcttgttcca gggcgaggag gcgcaccggt cctggctggg cctcaccccc tcgaccgacg
      601 tttacctcac aattccctac atcatagccg ctatctgctt catcatcggc atccggcggc
      661 taagctcccc caagacggcc cgcgccggta acaggatagc cgccgtgggc atgggcgtgg
      721 cggtggtggc taccttcttc agcagcagcg tgcagaatgt ctggttcata ctggccggta
      781 tcgtaatcgg cggcggggtc ggcttcttcc tggcccgcgc ggtgcagatg accgagatgc
      841 cccagatggt ggccctgttc aacgcgatgg gcggcggggc ggccgcgctc acttcgacgg
      901 tggagttcct ggagatcatc aaccgaggct ctacgcccga cctggacgtg gcgataagta
      961 cagtgctggg cgtgctcatc ggcgcgatga gcttctcagg cagcatcgtc gctatcgcca
     1021 agctttacgg ctggatttcc agcgcgccta tcacctatgg cgggcagcaa atcgtcaacg
     1081 cggtgctcgc gttgggggct gtgggcctcg gcgtgtggct cgtcatccag cctaacccct
     1141 tggcgctgat agggcttgtc gccctggcgg cggtactggg agtgttgctg gtgctgccca
     1201 tcggtggggc cgacatgccg gtggttatct cgctgctcaa ctcgttcacg ggtatggcgg
     1261 cggcctttac gggcttcgtg ctcagcaacc ccgtgctcat catcgcgggc gcgctcgtgg
     1321 gggcttcggg tatgctgctc accgtgctga tgggacgcgc tatgaaccgc tcggtgacca
     1381 acgtcatatt cggggcgttc ggctcggcgg gcgtggacgt gggcggtggc gccagcgcgg
     1441 ccggtaagtc ggtgcgcgag ctgggagtgg acgacgcggc cacgctgatg gcttacgcca
     1501 accaggtggt ggtcgtgccc ggctacggta tggctgtggc ccgcgcccag aacgcggtgc
     1561 gcgagctgtc cgacctcctc cagcagaagg gcgtcacggt gaagtacgcg atacacccgg
     1621 tggcggggcg catgccgggg cacatgaacg tgctgctggc cgaggctgat gtgccctaca
     1681 accagctatt tgagatggag gacatcaacc ctgagttctc ccgtaccgac gtggccctca
     1741 tcatcggggc taatgacgtg gtgaacccgg cggcgcgcaa cgaccccaaa agccccatct
     1801 acggtatgcc catactggac gtggacaagg cgcagaacgt gatcgtgatg aagcgcagca
     1861 tgcgcagcgg tttcgcgggc atcgagaatg agcttttcta cgaggatagg acgagcatgc
     1921 tgttcggtga cgcgaaggag atggccgagg cgctggtggc ttcggtcaag cagctgtaac
     1981 gggggcggtg tgaagttggt acaattgggg gtccggtagc agtgccggac cctcttttac
     2041 tgtttgggag ggcagggtgt tgtcattctt gggaggcggt aggtgacgcc ctgtggctga
     2101 agccacgggc tgagaaaagg caaagccccc tacggggact aaggaacatc aggtagtctc
     2161 cgaaggagac ttcgtctccg gtcagcccgc caattcattg gcagggcgag cgtggcagga
     2221 cgtcccaacc gcctctcagg taagaacgac cattccctgt tcccaaggaa atgtgagcta
     2281 aggaatgttt gcgctgcatt ggagggggag gcagcaacgt ggaggtgcaa tgagcgtatt
     2341 ggatgacgta aaagaggcgc tgggggagac cgtgcccgaa gtggcggtcg aggagcgcga
     2401 ggcagtggac gcgaggacgt acgactgcgc gattgtgggt ggcggccccg cagggttgtc
     2461 tgccgctatc tacatgggcc ggatgcgccg gagcgtggtg gtaatagacg acgaccaggg
     2521 tcgctcgaac tggcaccagg tgaaccgcaa ctacctgggc ttcgtggacg gcatacacgc
     2581 caagtctcta cgagaggtgg gagagaagca ggcgggcaag ttcggcgtgc gcttcctggc
     2641 tgccagggcc acggg
//
LOCUS       DASRWZ010000562         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_251450, whole genome shotgun sequence.
ACCESSION   DASRWZ010000562 DASRWZ010000000
VERSION     DASRWZ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251450"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFH60_08855"
     CDS             <1..293
                     /locus_tag="VFH60_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6313931.1"
                     /translation="SALGGAAGILYGMSLNSIEPGMGNQVELKGLSIIILGGMGSIPG
                     AMIAGFLIGMVEVLSVVFGGSQLKDALVFVALFLVLLLRPQGIFGARQGRAA"
     gene            470..1375
                     /locus_tag="VFH60_08860"
     CDS             470..1375
                     /locus_tag="VFH60_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6313932.1"
                     /translation="MVEFYNNNSFLINLIGINAIMALSIYITLSCAMLSLSNAASMAV
                     GAYASALLTQQAGWPLWLAVVAGAAVAGLVALPLGLPILRLRGVFLAIATLGFGEVVR
                     IIVVNTPAVGGPEGFRLNAGDRSATVSGLEIFGTLVVLAYFFARMKGSRMGLALDAIR
                     EDESAARTMGINVTFYKVVAYVTGAVVAGLGGALYAHLRAYISPGDFGFQRAVDILVF
                     AVVGGTFAWRGAILGAALITLLPEITRSAPVVGGLNIKDHPEIFSGLILLLVILFLPR
                     GLLAVPLGLKLRRPARLPAEKEAAP"
     gene            1372..2160
                     /locus_tag="VFH60_08865"
     CDS             1372..2160
                     /locus_tag="VFH60_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313933.1"
                     /translation="MTPQPAPIEGREPMLSVRDLTRSFGGLLAVSGVSFDIYPGEIYG
                     LIGPNGAGKTTVLNILSGLLPPSSGTITFQGQRIERLPAHRIARLGVARTYQNIRLFA
                     AMNVLQNVVVGQHSRMQGTLAERLAFSPRVRREEEAARQKALALLESVELASVAEEPA
                     SALAYGNQRRLELVRALASDPRLLLLDEPAAGMNAAESQTLGTRLRSLADQGLTLLVI
                     EHDVALVMSLCDRIGVLNFGNLIAQGTPDEIAANPEVIEAYLGT"
     gene            2235..2966
                     /locus_tag="VFH60_08870"
     CDS             2235..2966
                     /locus_tag="VFH60_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313934.1"
                     /translation="MQVTDLAVSYGQIEAVKGVTLQVEQGQIVALGGANGAGKSTTLA
                     AISGLLRPGRGSIKFEGVEISRWPSHRIVGAGIVQVPEGRAILAGMTVRENLELGASA
                     GGTRPVDQAGIGRMYERFPVLGTRRDGQAGNLSGGEQQMLAIARALLLKPRLLLMDEP
                     SMGLAPLLVREIFNIIAELNRDGSTILLVEQNSHKALQIAHYGYVLDRGRVALEGTGE
                     SLLHNEQVERTYLGGAATKEVPRKA"
     gene            complement(3070..>3659)
                     /locus_tag="VFH60_08875"
     CDS             complement(3070..>3659)
                     /locus_tag="VFH60_08875"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HET6313935.1"
                     /translation="LRGQVRRPLLGGVKGFLFLGRNVSIRHAHLLRTGRGVVLDDNVF
                     IDALGEWGVRLGDGVTIARGTVIKSSSVLWTASKGFVMGSNSSLGDYSFVGAAGGVRI
                     GSNVLGGQRLSFHSENHNYEDATRPIREQGVRREGIVVEDDCWLGSGAIFLDGVTVGR
                     GSVVAAGSVVTRPVPAYSIVAGVPAKVIGRRGSKA"
BASE COUNT          669 a         1166 c         1179 g          645 t
ORIGIN      
        1 cctcggcgct tggcggggcg gcgggcatcc tatacggcat gagcctcaac agcatcgagc
       61 ctgggatggg caaccaggtc gagctaaagg gcctctccat aatcatcctg ggcggcatgg
      121 gcagcattcc gggggctatg atagcgggct tcctcatcgg catggtcgag gtactgagcg
      181 tggtcttcgg cggctcccag ctaaaagacg ccctggtatt cgtggccctc ttcctggtgc
      241 tcctgctccg cccccagggc atcttcggtg cgagacaagg ccgagcagcg tgaaagtacc
      301 gagtgccgag tagagagacg aaaacgcaaa gcaggccctg agcgcagccg aagggacgca
      361 aagaacgcaa aggacgcgca gagattagga ttgtttaaga tatggtgtag gcatcaatgg
      421 ctgaccactg accgctaggt tctcggcact cggcactcgg cactgacgta tggttgagtt
      481 ctacaacaac aacagctttc tcataaacct gattggcatc aacgcgataa tggcgttgag
      541 catatatatc acgctgtcct gcgctatgct ctcgctgagc aatgcggcgt cgatggcggt
      601 gggggcgtat gcgtcggcct tgctgaccca gcaggcgggt tggccgttgt ggctggcggt
      661 cgtggcgggg gcagcggtgg cagggttagt tgcgctgccg ctggggctgc ccatattgcg
      721 gttgaggggg gtgttcctgg caatcgccac gctcggtttt ggggaggtag tgcgcattat
      781 cgtggtgaac acgcccgcgg ttggagggcc ggagggcttc aggctcaacg cgggcgaccg
      841 gtcggctact gtgtcgggcc tggagatttt cggcacgctg gttgtgctcg cctacttctt
      901 cgctcgcatg aaggggtcac gcatgggact ggccctggac gccatacgcg aggatgagag
      961 cgccgcccgc acgatgggca tcaacgtcac cttctataag gtggtggctt atgtcaccgg
     1021 cgcggtcgtg gcggggctag gtggtgcctt gtacgcgcac ctgcgagcgt acattagccc
     1081 cggcgacttc ggcttccaac gggccgtcga tatcctggtg ttcgccgtgg tgggcggtac
     1141 gttcgcctgg cggggggcca tactgggcgc ggctctcatc accctactac cggagatcac
     1201 ccgcagcgca cccgtcgtgg gcggcctgaa catcaaggac cacccggaga tcttcagcgg
     1261 cctcatcctc ttgctagtga tactgttcct ccccaggggc ctgctcgcag ttccgctggg
     1321 cctcaagtta cgccgccccg cccgtttgcc cgccgagaaa gaggccgcac cttgacccct
     1381 caacccgcac cgatagaagg acgcgagccg atgctgagcg tgcgcgacct tacccgctcc
     1441 ttcgggggcc tcctcgcggt gagcggcgtc tccttcgaca tctaccctgg cgagatctac
     1501 gggctgattg ggccgaacgg cgcgggcaag accacggtgc tgaacatact cagcggcctg
     1561 cttcctccct caagcggcac catcaccttc cagggacagc gtatcgaacg cctcccggcg
     1621 catcgcatcg cccggctggg ggtggcgcgc acctaccaga acatcaggct gttcgccgcc
     1681 atgaacgtgc tgcaaaacgt ggtggtgggg cagcactcgc gcatgcaggg cacactggcc
     1741 gagcggctgg cgttctcgcc ccgcgtgcgc cgtgaggaag aggcggcccg gcagaaggcg
     1801 ctcgcgctgc ttgagagcgt ggaactggcg agcgtggcag aggagcctgc atccgccctc
     1861 gcatatggca accagcgccg cctggaactg gtgcgcgccc tcgcctccga ccccaggctg
     1921 ctgctcctcg acgaacccgc cgcaggcatg aacgccgccg agagtcagac cctgggcacc
     1981 cgcctccggt ctctcgcgga ccagggactc accctcctcg taatcgagca cgacgtagcc
     2041 ctggtgatga gcctctgcga ccgcatcggc gtgctcaact tcggcaacct catagcccag
     2101 ggcaccccgg acgaaatcgc cgccaaccct gaggtaatcg aggcgtacct gggaacgtga
     2161 ggaattacga attacgaatt acgggatgaa caccgtaatt cgtaattcgt aattccccga
     2221 aggggccagc gaggttgcaa gtgaccgatc tggccgtgag ctacgggcaa attgaggcgg
     2281 tgaagggggt gaccctgcaa gtggagcagg ggcaaatcgt cgcgctcggg ggggccaacg
     2341 gggctggcaa gtccaccacg ctggcggcta tttcggggct gctgcggccg ggccggggca
     2401 gtatcaagtt cgaaggcgtg gagattagcc gctggccctc gcacaggatc gtaggggccg
     2461 gtatcgtgca ggtgccggag gggagggcta tactcgcggg tatgaccgtg agggagaacc
     2521 tggagcttgg cgcgtcggcg ggtggcacga ggcccgtgga ccaggcgggc atcgggcgca
     2581 tgtacgagcg gttccccgtg ctgggcaccc ggcgcgacgg gcaggcgggc aacctctcag
     2641 gcggcgagca gcagatgctt gccattgccc gcgccctcct cctcaagccc cgtctgctgc
     2701 ttatggacga gccttcgatg ggcctcgcac ccctgctcgt gcgcgaaatc ttcaacatca
     2761 tcgccgagtt gaaccgcgac ggcagcacca tcctcctcgt ggaacaaaat tcccacaagg
     2821 ctctgcaaat cgcccactac ggctatgtgc tcgaccgagg ccgcgtggcc cttgaaggca
     2881 cgggcgaatc gttgttgcac aacgagcagg tggagcgcac ctacctggga ggggcggcca
     2941 caaaggaagt accacgtaag gcgtaacgcg taacacgtaa tacgtagaaa agccaacaaa
     3001 tcagctagta agggacagag cctcttgggc cgccttacgt gttacgtgtt acgccttacg
     3061 ccttacgcct tacgccttac ttcccctgcg gcctatgacc ttcgcgggca cccctgccac
     3121 gatgctgtag gccggcaccg gccgcgtgac tacgctcccg gcggccacca ccgagccgcg
     3181 gcccactgtg acaccgtcca ggaaaatagc cccgctgccc agccagcaat catcctctac
     3241 gacgatgccc tcgcgccgca ccccctgctc gcgaatgggg cgcgtggcgt cctcgtagtt
     3301 gtggttctcg ctgtggaagc tgagccgctg gccgccaagc acgttggagc cgattctgac
     3361 gccccccgcc gcacccacga acgagtaatc gcctagtgag gagttgctgc ccattacgaa
     3421 gcccttgctg gcagtccaca ggaccgacga gcttttgatt acggtgccgc gagcaatcgt
     3481 gacgccgtct cccaggcgca cgccccactc gcccaaggcg tcgatgaaca cgttgtcgtc
     3541 gagcactacc ccacgccccg tcctgagcag gtgagcgtgc cggatcgaca cgttccgccc
     3601 gaggaacaag aaacccttta caccgcccag caagggccgc cggacctggc ccctgagga
//
LOCUS       DASRWZ010000563         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1074283, whole genome shotgun sequence.
ACCESSION   DASRWZ010000563 DASRWZ010000000
VERSION     DASRWZ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074283"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFH60_08880"
     CDS             <1..723
                     /locus_tag="VFH60_08880"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET6313936.1"
                     /translation="ISPRQYAEACRVRRLKESLRGGETVTNALYDSGYNSSSVLYGKA
                     GDQLGMTPATYRKGGTSTQISYTIAQSPLGRLLVAATERGVCAVRMGDDDARLEAALR
                     DEYHAAGIQRDDTVLGEWVEAILAHLSGRQPHLDLPIDVRATAFQWRVWEALRAIPYG
                     STRSYLQVATAIGQPSAVRAVARACATNPVALVVPCHRVVREDGSLGGYRWGMERKQA
                     LLDAERQAAGLSDSEDYSEAAG"
     gene            723..1604
                     /locus_tag="VFH60_08885"
     CDS             723..1604
                     /locus_tag="VFH60_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET6313937.1"
                     /translation="MKIGLVEVPYYFGREGVSMGAGPARYLEAGAAEELRQAGFEVRV
                     ERVRLDSPVEDDRNAIVEVNSRVASVVRELAGDGYFPLVLAGLCNTSIGVLGGVGAEG
                     TGVIWFDAHGDFNTPQTSTSGFLDGMALAMVTGNCHADLRVRAGQVRPVAETRVLLVG
                     PRDLDPGERDNLDASQVAVLEYQEVREGGLAPKLQLALEELQARTGEIYLHLDVDVLD
                     HDEAPANGYSTPGGLPLGQLEAGIREIAGRFTIKAATLSAYDPAYDEEGKTLKAGLRL
                     MRIVAREVSTATSSNVT"
     gene            1668..2627
                     /gene="yedA"
                     /locus_tag="VFH60_08890"
     CDS             1668..2627
                     /gene="yedA"
                     /locus_tag="VFH60_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004887901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HET6313938.1"
                     /translation="MAATNQAIDNTMPAPHGASEGKSNRLTIGLALLSLYLVWGSTYL
                     GIRIAIETFPPFMMGAIRFLVAGAALYAFLRLRGHANPSRAEWRGAAIMGVLLLVGGN
                     GMVIFAEQEISSALAALMIATTPLFTALFSGLWGNWPKRLEWGGIALGLVGVAMLTME
                     SDLQANPIGTLALIAATVSWSFGSIWSKRLSLPTGLMASAAEMLAGGAVFMLISLVLG
                     EHFKSAPSVASTSALVYLIIFGSLVAFSAYLYLLKNVRPALATSYAYVNPVVAVVLGV
                     GFAGEEITWFGIVGMLVILTGVASMSLVRDRGPKSEKAVKSST"
     gene            2657..>2889
                     /locus_tag="VFH60_08895"
     CDS             2657..>2889
                     /locus_tag="VFH60_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HET6313939.1"
                     /translation="MQSEPITVLRRSWCEDSQAAVEHFQQVGIEYTEVDIEEDEQAGD
                     WVKYLTGGHHITPTFVYRMQAAVLEPWDQARFER"
BASE COUNT          543 a          892 c          982 g          472 t
ORIGIN      
        1 atcagcccgc gccagtacgc cgaggcgtgc cgggtgcgcc gcctgaaaga gagcctgcgc
       61 gggggcgaga cggtcaccaa tgccctctac gactccggct acaactccag cagcgtgctc
      121 tacggcaagg caggcgacca gcttggcatg acccccgcca cctaccgcaa gggcggcacg
      181 agcacccaga ttagctacac catcgcccaa tctcccctcg gacgcctcct ggtggccgcc
      241 accgagagag gcgtgtgcgc ggtgcgcatg ggcgacgatg acgcccggct tgaggccgcc
      301 ctgcgcgatg agtaccacgc ggcaggcata cagcgcgacg acacggtgct gggcgagtgg
      361 gtggaggcga tactggccca cctgtccggc aggcagcccc acctcgactt gcccatagac
      421 gtgcgggcca cggcgttcca gtggcgcgtg tgggaggcgt tgcgcgccat accctacggc
      481 agcacccgct cctacctcca ggtagccacg gcgattggtc agcccagcgc ggtgcgtgcc
      541 gtcgcaaggg cgtgcgccac aaacccggtg gccctggtcg tgccgtgcca ccgagtggta
      601 agggaagacg gcagcctggg tggttaccgc tggggcatgg agcgcaagca ggcgctgctg
      661 gacgcagagc gtcaagcggc gggactctcc gattcggaag actactcgga ggcggcgggg
      721 taatgaaaat cggcctggtg gaagtaccgt actacttcgg gcgggaaggc gtctctatgg
      781 gcgccggtcc cgcccgctac cttgaggcgg gcgcggcgga ggagctacgc caggccgggt
      841 tcgaggtgcg ggtggagcgg gtgcgcctgg acagccccgt agaggacgac cgcaacgcca
      901 tcgtggaggt caacagccgc gttgcctctg tcgtgcgaga gcttgcgggc gatgggtatt
      961 tccctctcgt attggccggg ctgtgcaaca cgtcgatagg cgtgctgggc ggggtcgggg
     1021 cggagggcac gggcgtcatc tggttcgacg cgcacggcga cttcaacacg ccccagactt
     1081 caacgagcgg cttcctcgac ggcatggccc tggcgatggt caccggcaac tgccacgcgg
     1141 acttgcgcgt gcgggcggga caggtgaggc ctgtcgcgga gacgcgggtg ctcctggtgg
     1201 gtccccgcga cctcgacccc ggcgagcgcg acaacctgga tgcctcccag gtcgcggtgc
     1261 tagaatacca ggaggtgcgc gagggtggcc tggctccaaa gttgcaacta gcgctggagg
     1321 agctacaggc gcgcaccggc gaaatctacc tgcacctgga cgtagacgtg ctcgaccacg
     1381 acgaagcccc cgccaatggc tacagcaccc caggagggct gccactgggc caactggaag
     1441 caggcatacg cgaaatcgcg gggcgcttca ccatcaaagc cgccaccctg agcgcctacg
     1501 acccggctta cgatgaggag ggcaagacgc tcaaagcggg cctgcgactc atgcgcatag
     1561 tagcaaggga ggtttcgaca gcaacatcga gtaatgtgac ataaaaagcg tccaacaact
     1621 tagcaaacaa cattggacat ctaaaatcag gaaatggaaa tttaatcatg gcagcgacaa
     1681 atcaggcaat agacaatacg atgcccgccc cccacggcgc atccgaaggc aagagcaacc
     1741 ggctgacgat tggactggcc ctcttatccc tctacctcgt ctggggttcc acgtacctcg
     1801 gcatacgcat agcgatagag accttcccgc ccttcatgat gggggcgata cgcttcctgg
     1861 tcgcgggtgc ggcgctctac gccttcctgc ggctgcgggg gcacgccaac ccaagccggg
     1921 cggagtggcg cggggcggcg attatgggcg tgctcttgct ggtgggcggc aacgggatgg
     1981 tgattttcgc cgagcaggag atttcttcgg cacttgccgc gcttatgata gccaccacgc
     2041 ccctctttac ggcgctgttc agcggcctgt ggggcaactg gccgaagcgg ttggagtggg
     2101 gcggtatcgc gctggggctg gtgggggtgg cgatgctcac gatggagagc gacctgcaag
     2161 ccaaccccat aggcacgctg gccctcatcg ccgctaccgt ctcgtggtcg ttcggctcta
     2221 tctggagcaa gcgcctgtcg ctacccaccg gcctcatggc gagcgccgcc gagatgctgg
     2281 caggcggggc ggtctttatg ctgataagcc tcgtgttagg cgagcacttc aagagcgccc
     2341 catccgtcgc ctccacgagc gcgctggtct acctcatcat cttcgggtcg ttggtggcct
     2401 ttagcgcata cctctacctg ctgaagaacg tgcgcccagc cctcgctacg agctacgcct
     2461 acgtcaatcc ggtggtggca gtggtgctgg gggtgggctt tgcgggcgag gagatcacgt
     2521 ggttcggcat cgtgggtatg ctggtgattc tcacgggcgt cgcctcgatg tcgctcgtgc
     2581 gcgaccgtgg acctaaaagc gagaaagcgg taaaatcctc cacatagacc gacgtcaaca
     2641 tgaacgggag ggaaacgtgc agagcgaacc gatcacagtg ctacgccggt cctggtgcga
     2701 ggacagccag gcggcggtgg agcacttcca gcaggttggc atcgagtaca ccgaagtgga
     2761 catcgaggaa gacgagcagg caggcgactg ggtgaagtac ctcacgggcg ggcaccacat
     2821 aacgcccaca tttgtctacc gcatgcaggc agcggtgttg gaaccgtggg accaggcccg
     2881 cttcgagcg
//
LOCUS       DASRWZ010000564         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_869456, whole genome shotgun sequence.
ACCESSION   DASRWZ010000564 DASRWZ010000000
VERSION     DASRWZ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869456"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..2807
                     /locus_tag="VFH60_08900"
     CDS             360..2807
                     /locus_tag="VFH60_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET6313940.1"
                     /translation="MYVFDTEISSAVAQDGAKAEAPAVEQEPVEGPLSPELLDKMIRY
                     WNASNYLTVGQIYLQENPLLREPLHAEHIKPRLLGHWGTSPGLSLVYVHLNRLIKEHD
                     ANVIYLAGPGHGGPAIIAQVYLEGTYSEIYKEVTEDAEGIRQLFRRFSTPGGVPSHVS
                     VPTPGSIHEGGELGYVLTHAFGAAFDNPDLIVAAVVGDGEAETGPLMGSWQGISFLNP
                     VRDGAVLPILHLNGYKIANPTVLGRSSNEDVRSLLEGFGYEVHFVEGDDVPRVHQQFA
                     ATLDTCYARIREIQQDARTNGFSGRPRWPLIVLRTPKGWTGPKEIDGLPVEGTFRSHQ
                     VPLHQVKKKPDELAGLEEWLRSYRPGEAFDEQGRLIPELRELAPEGIRRMGANPHANG
                     GKLLVDLNMPDFRDYAIPVEAPASERHESTRQLGRMMRDIFKLNAEQANFRLFCPDET
                     HSNRLDNVFEVENRCFVGRTLPLDEHVSPAGRVMEVLSEHNCEGWLEGYLLTGRHGLY
                     ATYEAFAMVSASMTVQHTKWQQEGIELPWRDSIASLNILLTSTSWRNDHNGFSHQGPG
                     LIDVMLSKRGTVTRIYLPPDANCLLSVADHCFRSRNYVNLIVIDKQPQLQYLSMEQAI
                     EHCARGASVWEWAGNDAGVEPDVVLACAGDIPTMETLAAAWWLRKNIPELKVRVVNVV
                     DLMTLFPRSVHPHGMSETDFVNTFTADKPIIFAFHGYQRAIHQLVHGRPNADRFHVRG
                     FIEQGTTTTPFDMVVLNEMSRYHLVISALKYATRVRGLAAPLVDECNTLIQRAVQYSR
                     EHFEDMPEIRNWVWSQP"
BASE COUNT          599 a          957 c          855 g          479 t
ORIGIN      
        1 aacaaagccg tcagatatgt gattgcgagg cgcttaccag cagccaacag tgccacaaac
       61 accaggacca aaacccccct ttgtcattcc gagcggagcg aggaatcagg tggtcgtcgt
      121 acattgtcac agatagaagg accaagcaac gtaagtccac tctactcctc caattcacct
      181 tcttccctac ccacctgatt cctcactgcg ttcggaatga caaaaggggc tttggtcctg
      241 gtgattgtag caccgtagga aggtcaagaa agccctgtct cccttataat gggctgacaa
      301 cccaccgctt gttcattcgc ctgtgattgt tattcttggc tgtgaaagag agaaccttta
      361 tgtacgtatt cgatacagaa atttccagcg cggtcgccca ggatggagca aaggccgaag
      421 ccccggcagt ggagcaggag ccggtggaag gccccctctc cccggagctg ctggacaaga
      481 tgatccgcta ctggaacgcc tctaattacc ttaccgtggg acagatctac ttgcaggaga
      541 atcccctcct gcgggaaccc ctgcacgccg agcacatcaa gccccgcttg ctggggcact
      601 ggggcacctc gccggggcta agcctggtat acgtgcacct caaccggctc atcaaggagc
      661 acgacgccaa cgttatctac ctcgcggggc cgggacacgg cgggcctgcc atcatagcgc
      721 aggtgtacct tgagggcacc tactcggaaa tctacaagga ggtcacggag gacgcggagg
      781 gtatccgcca actgttccgc cgattctcga cccccggcgg cgtgcccagc cacgtaagcg
      841 tgcccacccc cggctccatc cacgagggtg gcgagcttgg ctacgtgctc acccacgctt
      901 tcggcgcggc cttcgacaac cccgacctca tcgtggcggc ggtcgtgggc gatggcgagg
      961 cagagacagg cccgctcatg ggttcgtggc agggcatcag cttcctcaac ccagtgcgcg
     1021 acggcgcggt actccccatc ctgcacctca acggctacaa aatcgccaac cctaccgtgc
     1081 tagggcggtc cagcaacgag gatgtgcgct ccctgctgga aggcttcggc tacgaggtgc
     1141 actttgtcga gggcgacgac gtaccccgcg tgcaccagca gttcgccgcc acgctcgata
     1201 cctgctacgc ccgcatccgc gagatacagc aggacgcccg cacgaacggc ttttcaggcc
     1261 gcccgcgctg gcccctgatc gtgttgcgca cgcccaaggg ctggaccggg ccgaaggaga
     1321 tagacggcct gccggtcgag ggcaccttcc gctctcacca ggtgccgcta caccaggtga
     1381 aaaagaagcc ggacgagctt gcggggcttg aggagtggct gcgcagctac cgtcccggcg
     1441 aggcgtttga cgagcagggc aggctcatac cagaactgcg ggaattggcc cccgaaggca
     1501 tccgccgcat gggcgccaac ccccacgcca acggcggtaa gctgctggtg gacctcaaca
     1561 tgcccgactt ccgggactac gccatcccgg tggaagcccc cgcgagcgag cgccacgaat
     1621 cgacccggca gttgggcagg atgatgcgcg acatcttcaa gctgaacgcc gagcaggcca
     1681 acttccgcct cttctgcccc gatgaaacgc actccaacag gctggacaac gtattcgaag
     1741 tggagaaccg ctgcttcgta ggccgcacgc tgcccctgga cgagcacgtg tcgcccgcgg
     1801 gccgcgtgat ggaggtcctg agcgagcaca actgcgaggg ctggctggaa ggttacctcc
     1861 tcaccgggcg gcacgggctg tacgcgacgt acgaggcgtt cgctatggtg tcggcctcca
     1921 tgacggtgca gcacaccaag tggcagcagg agggcatcga gctaccctgg cgcgactcta
     1981 ttgcctccct caacatcctg ctcacctcga cctcctggcg caacgaccac aacggcttca
     2041 gccaccaggg gccgggcctc atcgacgtga tgctctccaa gcgcggcacg gtgacccgta
     2101 tctacctgcc gcccgacgcc aactgcctgc tctccgtggc cgaccactgc ttccgcagcc
     2161 gcaactacgt caacctgatc gtgatcgaca agcagccgca gctacagtac ctgagcatgg
     2221 agcaggcgat agagcactgc gcgaggggcg cgtcggtctg ggagtgggcg ggcaacgacg
     2281 cgggggtcga gccggacgtg gtgctggcct gcgctgggga catccccacg atggagacgc
     2341 tcgccgccgc ctggtggctg cgcaagaaca tccctgagct gaaagtccgc gtggtgaacg
     2401 tggtggacct gatgacgctc ttccctcgct cggtgcaccc gcacggcatg agcgagaccg
     2461 atttcgtgaa cacgttcacc gctgacaagc cgataatatt cgcgttccac ggctaccagc
     2521 gggccataca ccaactggtg cacggccgcc ccaacgccga ccgcttccac gtgcgcggct
     2581 tcatcgagca gggcaccacc actaccccct tcgacatggt ggtgctcaac gaaatgagcc
     2641 gctaccatct cgttatctca gccctgaagt acgctacccg cgtacgtggc ctcgccgccc
     2701 cgctcgtgga cgagtgcaac accctcatcc agcgtgccgt gcagtactcc cgcgagcact
     2761 tcgaggacat gcccgaaatc cgcaactggg tctggtccca gccttagaga cgaaaacgca
     2821 aagacgcaaa ggacgcaaag aacacgcaaa gagagtgaag gtttagagga cagagagaag
     2881 cagggtacgg
//
LOCUS       DASRWZ010000565         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_800454, whole genome shotgun sequence.
ACCESSION   DASRWZ010000565 DASRWZ010000000
VERSION     DASRWZ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800454"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFH60_08905"
     CDS             <1..384
                     /locus_tag="VFH60_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313941.1"
                     /translation="YRVIESIEGRCDDICYFLAERGTRPFFPDTIRRMVLLASPEITG
                     YQAFQERPGHLRIHLELPPASSFDKVANDVRESVNTIVAQYGCMPAEVEVKLGLLPTT
                     PGTKRRRVVNLQTTQHPNEQPSATR"
     gene            413..1723
                     /locus_tag="VFH60_08910"
     CDS             413..1723
                     /locus_tag="VFH60_08910"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313942.1"
                     /translation="MSEAKGSNSQISIIALGSRGDVQPFVALGLGLRAAGHRVRIAAA
                     ADYAPLVEAYGLEFAPLAGSIRELMDREMVYGILDAPGNPLRFGKRMLDAVQPIVNRL
                     VADCREACVGADMLIVSTLGVYVAYDLALDLGIPFYAAHMHPYGTTRAYPQVFFPSLP
                     GWVPLRGRYNRLTHGLAEAGFWLLLSAALNAAREALALPATSRASMLRRAAEITDPTL
                     LAYSPGIAPRPRDWQARTRVTGYWFLDRPAGWQPPALLSDFLASGPPPVYVSFGSNLA
                     GRDPDEVTRLLLGALKQAGVRGIVSSGWGDLGNIDLPDTVLRVEQVPHDWLFPRMAAV
                     VHHGGAGTTAAALRAGVPSVAVPFFGDQRFWAGRLHALGAAPAPLPRPQLTADRLAGA
                     IRAAVCNPAMRQRASWLGERLNKENGVKRAIAALRLDRCGYDVA"
     gene            2045..>2630
                     /locus_tag="VFH60_08915"
     CDS             2045..>2630
                     /locus_tag="VFH60_08915"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET6313943.1"
                     /translation="MLPVKIAGLGWYLPERRVPNAELEERLQIRAGWIEQVTGVRERR
                     YATGETSVIMGGAACRMSLADAGLDVDDIDAIIGASAAPQQMIPCTAALMQRELGAPD
                     GGSACFDVNATCLGFLFALQTASLLVASGAYRNVLIFSSEIGSRSLNPKERESAVLFG
                     DAAAAAVLTRSGPGESSAVWHSQFTTYSSGADLTE"
BASE COUNT          454 a          862 c          841 g          473 t
ORIGIN      
        1 tacagggtga tagagtccat cgagggccgt tgcgacgaca tttgttactt cctggccgag
       61 cggggcacgc gccccttctt ccccgacacg atacgccgca tggtactcct ggccagcccc
      121 gaaatcaccg gctaccaggc cttccaggag cggcctggcc acctgcgcat ccaccttgag
      181 ctaccccccg cctcctcatt cgacaaagta gccaacgatg tacgagaaag cgtcaacacc
      241 atcgtagccc aatacggctg catgcccgcc gaggtagaag taaagctcgg cctgctcccc
      301 acaacccccg gcaccaaacg ccgccgcgtc gtaaacctcc aaacaaccca gcaccccaac
      361 gagcaaccca gtgccacacg ctaaaccaaa attcgaaatt cgaagcttgc ccttgagcga
      421 agcgaagggt tccaattccc aaatctcgat catcgctctc ggttcgaggg gcgatgtgca
      481 gcctttcgtg gcgttgggtc ttggcttgcg ggccgccggg caccgagtac gcattgccgc
      541 cgctgccgat tacgcccccc tggtggaggc atatgggctg gagttcgcgc ccctggcggg
      601 cagcatccgc gagctgatgg accgggagat ggtgtacggc atcctggacg cgcccggcaa
      661 cccactacgg ttcggtaagc gcatgctcga cgcggtccag cctatcgtca accgcctggt
      721 cgcggactgt cgcgaggcgt gcgtgggggc ggacatgctg atcgtctcga ccctgggcgt
      781 gtacgtggct tacgacctgg cgcttgattt gggcataccg ttctatgcgg cgcacatgca
      841 cccctacggc accacgcgcg cctacccgca ggtcttcttc cctagcttgc ccggctgggt
      901 gccgctgcgg ggccgctaca accgcctcac gcacgggctg gccgaggcgg gcttctggct
      961 tctcttatcg gcagccctca acgcggcgcg ggaggcgctg gccctacctg ccacctctcg
     1021 tgcctcgatg ctgcgcaggg cggccgaaat taccgatcca acgctgctcg cctacagccc
     1081 cggcatcgcc ccgcgtcctc gcgactggca ggcgcgcacc cgcgtgaccg gctactggtt
     1141 cctcgaccgc ccggcaggct ggcagccgcc cgcactcctg tccgacttcc tggcttcggg
     1201 gcctcccccc gtctacgtca gcttcggcag caacctggcg ggacgcgacc ccgacgaggt
     1261 cacgcggctg ctgctggggg cgttgaagca ggcgggcgtc cgcggcattg tgtcgagcgg
     1321 ttggggcgac ctgggcaaca tcgacctgcc ggacacggtg cttcgcgtcg agcaggtgcc
     1381 tcacgactgg ctatttcctc gcatggcggc ggtggtgcat cacgggggcg cgggcacgac
     1441 ggcggctgcg ttgcgggcgg gcgtaccctc ggtggccgta cctttctttg gcgaccaacg
     1501 cttctgggct gggcggctgc acgccctcgg tgccgccccc gcgcccctac ccaggccgca
     1561 actgaccgcg gaccggctgg ctggcgcgat tcgggcggcg gtgtgcaacc cggcgatgag
     1621 gcagcgcgcc tcatggttgg gcgaacgtct aaacaaggag aatggagtga aaagggctat
     1681 tgcggccctc cggttagaca ggtgtggata tgatgtggcg tgatgagtga tgagtgataa
     1741 gtcaggtggt tagctaacga tggtgtgtga gtgtgttaga gtgggtgttg cgggaccaac
     1801 tccccctttg tcattctgag cgtagcgaag aatctcgtcc ttcagcaccc aaataccacc
     1861 tttctttacg cagattaagg tgctatggtg ctatggaaaa ggggaacatc ctggtggggg
     1921 acgagattct tcgctacgct cagaatgaca aagaccaaca acgtccgagc atctcaacct
     1981 cgtaacattc acgcactacc cacggtaagc cgcctcactc ggcactcgta acaaggggga
     2041 tcatatgctt ccagtgaaga tagcgggcct cggctggtac ttgcccgagc ggcgggtgcc
     2101 taacgccgag cttgaggagc ggctgcaaat ccgggcgggt tggatcgagc aggttacggg
     2161 cgtgagagag cggcgttatg ccaccggcga gacctcggta atcatgggcg gggccgcgtg
     2221 ccgcatgtcg cttgcggatg ccggtctcga cgtggacgac attgacgcca taatcggggc
     2281 ctccgccgcg ccgcagcaga tgataccgtg cacggcggct ctcatgcagc gcgagcttgg
     2341 ggcacccgac ggcggcagcg cctgcttcga tgtcaacgcc acgtgcctgg gcttcctgtt
     2401 cgccttgcag acggcctccc tcctggtggc ctcgggcgcg taccgcaacg tgctcatctt
     2461 cagcagcgaa atcggctcgc gttcgctcaa tcccaaggag cgggagagcg cggtgctgtt
     2521 cggggacgcc gccgctgccg ccgtgcttac aaggagcggg ccgggggaga gcagcgcggt
     2581 atggcactcg cagttcacca cctacagcag cggggccgac ctcaccgaga
//
LOCUS       DASRWZ010000566         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_457955, whole genome shotgun sequence.
ACCESSION   DASRWZ010000566 DASRWZ010000000
VERSION     DASRWZ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457955"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1537
                     /locus_tag="VFH60_08920"
     CDS             <1..1537
                     /locus_tag="VFH60_08920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6313944.1"
                     /translation="VSGPEPSRQTLDYAVQMVQRAGRFRGRLSLTRRDNKQISVEAYC
                     TAYRAADGTEQVCITFHEVATTTDSADPVFQLAPVLESSNDAIIAMTLDGVILTWNSG
                     AEQLYGLTASEMKGRHIHEVVPPDLHGEMRAAFKKIVQGKYARTLEMHYLRSDGQGMH
                     LSLAFSPVHDAVGRLVGVSGIAHDITNRKQKEQVLERAEREYRGLFEHAQDAIVIFEP
                     EGEVILDVNERACDLYGLSRAELIGMSVQDLSHDVQHGKEQVDETLAAGTNRRFQAMH
                     YRSDGTLLHLDISAAVVSYQGRRAILSSNRDITEFKRLEAALRELSIRDELTRLYNRR
                     EMSRIVSEEVERCRRYNRDMSLLMIDIDWFKRVNDTWGHQAGDEILQGIARLLSENTR
                     VTDHVARYGGEEMVVILPETAGKQALVVAEHLRCKVAEHEFEVSGGAEDDSVEPLKLS
                     VSISLGLASYTGEFNTEEALLVAADRALYEAKRKGRNCTVQFSRSMLKTRQLAEANAA
                     RPE"
     gene            complement(1776..2378)
                     /locus_tag="VFH60_08925"
     CDS             complement(1776..2378)
                     /locus_tag="VFH60_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6313945.1"
                     /translation="MHPRELFLTLHAHVHAAATSGEHGPSLEDGLCDGLTDEQMRARP
                     DGFNSIVWLLWHTTRFEDVVVNTVLRGVPEVLDRDGWQQRLGVDTRLVGTGSSDEEVE
                     EFSTQVDPSAVRAYRAAVGRETRQWAETADFEALAAVPDVLGRLSQAPTALDERAEWV
                     RSLWANRTGLGLLSLPVIGHSYIHIGEARVTRSRLGGRVP"
     gene            complement(2442..3566)
                     /locus_tag="VFH60_08930"
     CDS             complement(2442..3566)
                     /locus_tag="VFH60_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6313946.1"
                     /translation="MTPTSISGYRHLFPVTEHYTYLNHAACTPMSRPGLEALARYWAE
                     QSTQGVLSEPKYFPVIDQAREKMARLIGAEPSEVGWLQNTSTALNMVANGLEWQAGEN
                     VVTVQGEFPANIYPWLGLARFGVETRLVAQRDNRVLVDDIAAAMDGNTRMLSISFVEF
                     ATGFRNDLCALGQLCRERGVLFTVDAIQGLGALQLDVHAAGIHFMGAGAHKWLMGPQG
                     VGVFYAHRDAMPLLRPLTANWYSVVNRDDYLTYGQPWVNANSRIEGSTHNVSGLVAFD
                     AILAMLLEVGPARIEERVLHLTARLIEGLRSKGYELVSSDREGERSGVVCFRAQGDPL
                     DVLARAQAENIVVAVRVGVVRVSPHFYNTEEEMDHFLSVL"
BASE COUNT          707 a         1195 c         1208 g          659 t
ORIGIN      
        1 cgtcagcggt cccgaaccga gcaggcagac cctcgattat gccgtgcaga tggtgcagcg
       61 ggcgggcagg ttccggggca ggctctcact cacccgcagg gacaacaagc agatctcagt
      121 tgaggcttac tgcaccgcct accgcgccgc cgatggcacg gagcaggtgt gcatcacctt
      181 ccacgaagtc gcgaccacca cggacagcgc cgacccggtc ttccagctcg ccccggtgct
      241 agagtcctcc aacgacgcca tcatcgccat gaccctcgac ggggtgatac tcacctggaa
      301 ctccggggcg gagcagcttt acgggctgac cgcgtccgag atgaagggga ggcacatcca
      361 cgaggtggtg cctcccgatt tgcatgggga gatgcgggcg gccttcaaga agatcgtgca
      421 gggcaagtac gcgcgcaccc tggagatgca ctatctcagg agcgacgggc agggcatgca
      481 cctgtcgctc gccttctcgc ccgtgcacga cgcggtgggc cgcctggtgg gcgtttcggg
      541 catcgcgcac gacatcacca accgcaagca aaaggaacag gtgctggagc gtgccgagcg
      601 cgagtacagg ggcctgttcg agcacgcgca ggacgccatc gtcatcttcg agccggaggg
      661 agaggtcatc ctggacgtga acgagcgcgc ctgcgacctc tacggcctga gccgagccga
      721 gcttatcggc atgtcggtgc aggacttatc gcacgacgtg cagcatggta aggagcaggt
      781 agacgagacg ctggcggcgg gcaccaaccg gcgcttccag gcgatgcact atcggagcga
      841 cggcaccctg ctacacctcg atatcagcgc ggcggtggtc agctaccagg gccggagggc
      901 cattctcagc agcaaccgcg acattacgga gttcaagcgc ctggaagccg ccctgcgcga
      961 gctttcgatc cgcgacgagc ttacccgcct gtacaaccgc cgcgagatga gccgcatcgt
     1021 ctcggaggag gtcgagaggt gcaggcgcta caatagggat atgtccctgc tgatgataga
     1081 catcgactgg ttcaagcggg tgaacgacac ctggggccac caggcgggcg acgagatatt
     1141 gcaaggcata gcgcgcttgc tctcggagaa cacccgcgtc acggaccacg tagcccgcta
     1201 cggcggagag gagatggtgg tcatcctacc ggagacggcg gggaagcaag ccctggtcgt
     1261 ggccgagcac ctgagatgca aggtagcgga gcacgagttc gaagtgagcg gcggagcgga
     1321 ggacgacagc gtcgagccgc tgaagctgag cgtgagcata agcctgggcc tggcgagcta
     1381 cacgggggag ttcaacacgg aagaggcgct gctcgtggcc gccgaccgcg ccctgtacga
     1441 ggccaagcgc aagggacgca actgcacggt gcaattctcc aggtcgatgc tcaagacccg
     1501 ccagcttgcc gaggccaacg ccgcgcgtcc tgaatagcaa ttgattaaga gacggaaccg
     1561 ccaagacgcc aaggacgcca agagcgcgcc aagttaagta cggtgttcga agcaaaaaga
     1621 acgagggtac attatcagta ctctggttct ttttgcacca ccctaacctt tattctctca
     1681 gcgcgtcctc tgcgttctct gcgtctctgc ggtgaaccgt tcttaatctt ggcgcgctct
     1741 tggcgtcctt ggcgtcttag cggttccgtc tccttctatg gcacgcgccc gcccagccgg
     1801 gagcgggtaa cgcgggcctc tccgatgtgg atgtagctgt ggcctatgac gggcaaggat
     1861 agcaggccga ggccggtcct gtttgcccac aggctccgca cccattcggc ccgctcgtcg
     1921 agcgcggtcg gggcctggga gaggcggccc agtacgtccg gtacggcggc cagggcctcg
     1981 aaatcggcgg tctcggccca ctgccgcgtc tcccggccca cggcggccct gtaggctctc
     2041 acggcggagg ggtccacctg cgtgctgaac tcctcgactt cctcgtcgct gctgccggtg
     2101 cctaccaggc gggtgtctac tcccaacctc tgctgccagc cgtcgcggtc gagcacctcc
     2161 ggcacgcccc gcaacaccgt attgaccacc acatcctcga accgggtggt gtgccagagc
     2221 agccacacta tcgagttgaa gccgtccggc ctggcccgca tctgctcgtc cgtcaggcca
     2281 tcacaaagcc cgtcctccag cgacggcccg tgctcgccgc tggtagccgc agcatgtacg
     2341 tgggcatgca gagtaaggaa caattcacga gggtgcattg ggcctccggg gaatttggaa
     2401 tttcggattt tggattttgg attttggaga gtgacgtagc attacaggac cgacaggaag
     2461 tggtccatct cctcttcggt gttgtagaag tggggggaca cccgcaccac gccaactcgc
     2521 accgccacga cgatgttctc ggcctgggcg cgggccagca cgtcaagcgg gtcgccctgg
     2581 gcacggaagc agacgacgcc ggaacgctcg ccctctctgt cggagcttac cagttcgtag
     2641 cctttgctgc gcagcccctc gatgaggcga gcggtgaggt gcagcacgcg ctcctctatg
     2701 cgagcggggc cgacctccag gagcatggcg agtatagcgt cgaaggccac gaggccgctg
     2761 acgttatggg tgctaccctc gatgcgcgag ttggcgttca cccagggctg gccgtaggtg
     2821 aggtagtcgt cgcggttgac cacgctgtac cagttggcgg tgaggggccg gagcagcggc
     2881 atcgcgtcgc ggtgcgcgta gaacacgccc actccttgcg gccccatgag ccacttgtgc
     2941 gccccggccc ccatgaagtg tatgcccgcc gcgtgcacgt cgagttgcaa agccccaagc
     3001 ccctgtatgg cgtccaccgt gaagagcacc ccacgctccc ggcaaagctg tcccagcgcg
     3061 cacaggtcgt tgcggaagcc ggtggcgaac tccacgaagc tgatgctcaa catgcgcgtg
     3121 ttgccgtcca tcgcggccgc aatatcgtcc accagcacgc ggttgtcgcg ctgcgccacc
     3181 aggcgtgtct ctacgccaaa gcgcgccagc cccagccagg ggtagatgtt ggcggggaac
     3241 tccccctgca ccgtgaccac gttctccccc gcctgccact caaggccgtt agccaccatg
     3301 ttgagcgcgg tggaggtatt ttgcagccaa cccacctcag acggctcggc cccaatcagc
     3361 cgggccatct tctcacgcgc ctggtcgatc accgggaagt acttcggctc gctgagcacc
     3421 ccttgcgtgc tctgctcggc ccagtaccgg gcaagggcct ccagccccgg cctcgacatg
     3481 ggcgtgcacg ccgcgtggtt caggtacgta tagtgctccg tcaccggaaa caggtggcgg
     3541 tagccggaga tagacgtcgg cgtcatcgat aaccttcttt gcgagtggct agtggtcagt
     3601 ggtcagtggt tagtaaggca acttggcgcg ggcagtgcgt gagtgtgtga cagtgggtgt
     3661 tgcaggacta agcccctatt gtcattctga gcgtagcgaa gaatctcgtc ctccagcacc
     3721 caaataccaa ttttcttttc tgcattagcg gataatagtg ctactgaaa
//
LOCUS       DASRWZ010000567         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_663360, whole genome shotgun sequence.
ACCESSION   DASRWZ010000567 DASRWZ010000000
VERSION     DASRWZ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663360"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1509)
                     /locus_tag="VFH60_08935"
     CDS             complement(157..1509)
                     /locus_tag="VFH60_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET6313947.1"
                     /translation="MDNTPGPVAAPSQPPQSGGQDQYAPVAPTPPGGPVPLGPTQQGY
                     GQPYGEAQVPARPGPGASNRAVLVTSGLALVGITMIGAGFLVGGEAAKFMTIGLQFIP
                     LAVLAALAFAGVRNRPASVLTYLWLAMLALGVLLLVGSYTVLGTVRDIRGLSQWLSNP
                     REVPLSEVFKPDAGMVVLLCLVLMSLVSLLSAAMLFRPVRVLVARVIPIDPDNFVHKI
                     ALSFLTLVTLGSFLPLIVLGGQPPLLLLINAPSTEGAIGQALDNAVRPQDLIYQFVWT
                     IPATFVAAGWPVARKFKAVLERLGFVRPTLLQVGGAVAGGVVLAAVAAFGLDPAITQL
                     WRAMGWGVTDVAAFGELMRQLITPEGAVLIGVTAGVGEELAVRGLLQPRIGLIASNLV
                     FTSLHAFQYGADALLSVFIIGMVLGLIRMRTNTTTSAIVHGVYDFVLVMASTSLFMGQ
                     "
     gene            complement(1668..2750)
                     /locus_tag="VFH60_08940"
     CDS             complement(1668..2750)
                     /locus_tag="VFH60_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET6313948.1"
                     /translation="MSDDNHTNTPGNDQANETRELAAPPAEQGSGGAPGEASSEGEPQ
                     DLDSMVDVHSVLADTDAPGEQASPVQEAAAAVAETAQAVTGPVAEAIAPVVQSTVQAT
                     QTAVQATRQATQQVTAAVQQQAQKITVAPRRAIYRGKRFLAVYGGLVGAAGLLAILAK
                     RHRYFPADVGITKALQKPNAKLYDALMHAVSELGWRWISVGTRASLSTLMWAAGFRME
                     SAFTVSTWSGDLMTIAIKTRVLRPRPTKDLVRVMGDLDEASFPSGHVVHYVTFYGFLF
                     YLVFIHLKQRWFRTALLTLLAGIILLVGPSRIYMGHHWPSDVGGAYLVGTLWLGVIII
                     AYLETKAHYTLHTSPPFFVKRPQQLG"
     gene            2970..3275
                     /locus_tag="VFH60_08945"
     CDS             2970..3275
                     /locus_tag="VFH60_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313949.1"
                     /translation="MTSWNRGRENEEIGEQQLNDGDPGNTLEGAARKVGGEIEQGLDN
                     LGDTLTGKQNDLQGKDQNWGNRAGDAIDDAGDNLRNASDNAGDWIENRGDDIRDATR"
     gene            complement(3404..>4269)
                     /locus_tag="VFH60_08950"
     CDS             complement(3404..>4269)
                     /locus_tag="VFH60_08950"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET6313950.1"
                     /translation="PRLVELFSLLCHHIYGSLPNMEAHGEAGPGKVNGGVYNLRPSFR
                     ARLNRTHQKRRMQEYQVVKPLELNSPTTVVVPVYDIEVECDSHSFIANNAIVHNSICT
                     TRVVSGAGMPQITAVWECYKVCREAGVPVIADGGIQYSGDIAKAIAAGADTVMLGSLL
                     AGVDESPGEIILYQGERFKEYRGMGSLGAMKQRSFSKDRYGQHEVEAISKLVPEGIEA
                     RVQYKGPLGTLLYQLVGGLRSGMGYLGAPDIKTLQEQARFIRISGSGLRESHPHDVTI
                     TKEAPNYGRSY"
BASE COUNT          860 a         1292 c         1361 g          756 t
ORIGIN      
        1 ggacggcctt tgtccttcca attcggcgtt gttccatgta tgtaaattgt gcctgggtgg
       61 gaaacatatg tgctagctac agatgccagg ataacaaaag acccaggtgc gcgcctgggc
      121 ctctggttgt atctttggca cgtcgggtgc ggttttctac tggcccatga agaggctagt
      181 gctcgccatt accagcacga agtcgtacac accgtgcact atggccgagg tggtggtgtt
      241 ggtgcgcatg cgtatgaggc ccagcaccat gccgatgatg aacacgctca gcagggcgtc
      301 cgccccatac tggaaggcgt gtagcgaggt aaagaccagg ttggaggcta tcaggccgat
      361 gcgcggctgc aacaggcccc tcaccgcgag ttcctcccct acccccgccg tcacgccgat
      421 gagcaccgca ccctccgggg tgattagctg gcgcatcaac tccccgaacg ctgccacgtc
      481 ggtgacgccc cagcccatcg cgcgccatag ctgggtgatg gcggggtcga ggccaaaagc
      541 cgccaccgcc gccagcacca cgccacccgc gacggccccc cctacctgga gcagggtagg
      601 acgtacgaag cccaggcgct ccagtaccgc cttgaactta cgcgccaccg gccaccccgc
      661 agccacgaag gtagccggta tcgtccacac gaactggtaa atcaagtctt gcgggcgcac
      721 agcgttgtcg agggcctggc ctatcgcgcc ctcggtagac ggtgcgttta tcagcaacag
      781 gagcggcggc tgcccaccca gcacgatgag cggcaagaag gagccaagcg taaccaacgt
      841 aaggaaggac agggctatct tgtgcacgaa gttgtcgggg tctatcggta ttacccgtgc
      901 tacgagcacc cgtaccggcc tgaacagcat cgcggccgaa aggagcgaca ccagcgacat
      961 gagcacgagg cagagcaaca cgaccatgcc cgcgtccggc ttgaacacct cggatagcgg
     1021 cacctcccgt gggttggaga gccattggga cagcccgcga atgtctctga cggtgccgag
     1081 cacggtatag ctgcctacga ggaggagcac accgagcgcc agcatcgcca gccacaggta
     1141 ggtcaggacc gaggccggcc ggttcctcac gcccgcgaaa gcgagtgcag ccagcacggc
     1201 cagcggtatg aactggaggc ctatcgtcat gaacttggcc gcctcgcctc caacgaggaa
     1261 gcccgccccg atcatagtaa tgcctacgag cgccagcccg gacgtgacaa gcaccgccct
     1321 gttggacgca cccggccccg gccgcgcagg cacctgggcc tctccataag gctgcccata
     1381 cccttgctgt gtcggtccga gcggcaccgg cccgccaggg ggcgtcggtg ccacgggtgc
     1441 gtactggtcc tgcccgccgc tttgaggtgg ctgcgaaggg gccgcgaccg gccctggtgt
     1501 gttatccatc aaatagtctt ctccataggg acgctagcta ccctcagtat aagggctgat
     1561 agcggccccg cacaggaccc aaaagggcta tttagaaatt agaatttcgg atttcgaatt
     1621 tagtagataa ctaatcaaat tcgaaatccg aaatccgaaa ctcgaaatca acccagctgt
     1681 tgcgggcgct tgacgaagaa gggagggctg gtgtggaggg tgtaatgggc cttggtctcc
     1741 aggtaggcga tgatgattac gcccagccag agggtgccca cgaggtacgc gccgcccacg
     1801 tcggagggcc agtggtggcc catgtagatg cgcgaggggc cgacgaggag gattattccg
     1861 gccagcagag tgagcagggc cgtgcggaac cagcgctgct tgaggtggat gaagacgagg
     1921 tagaagagga agccgtagaa ggtgacgtag tgcaccacgt ggccggaggg gaagctcgcc
     1981 tcgtccaggt cgcccatcac gcgcacgagg tccttggtag ggcgggggcg cagcacgcgc
     2041 gtcttgatcg cgatggtcat caagtcgccc gaccaggtag agacggtgaa ggcgctctcc
     2101 atgcggaagc ccgcggccca catgagggtg ctgagggaag cgcgggtgcc cacgctaatc
     2161 cagcgccagc ctagctcgct caccgcgtgc atcagggcgt cgtagagttt ggcgttgggc
     2221 ttctgcaagg ccttcgttat gcccacgtcg gcggggaaat agcggtgccg cttggcaagt
     2281 atcgccagca ggcccgccgc gcccaccaac ccgccgtaga ccgccaggaa gcgcttacca
     2341 cgatagatgg ccctgcgcgg ggcgaccgtg atcttctggg cctgctgctg gaccgccgcc
     2401 gtgacctgct gggtggcctg gcgcgtggcc tggaccgccg tctgcgtagc ttgcacggtg
     2461 gactgcacca ccggggcgat agcctccgcc accgggccgg tcacggcctg ggccgtctcg
     2521 gctaccgctg ccgccgcctc ctgcacgggc gaagcctgct cgccgggggc gtccgtgtcc
     2581 gccagtacgg aatgcacgtc caccatgctg tcgaggtctt gtggctcgcc ttcggatgaa
     2641 gcttcgccgg gcgcgccgcc actcccctgt tcggcaggag gggccgcgag ttcccgcgtt
     2701 tcgttggctt gatcgttacc gggcgtgttc gtgtgattgt cgtcggacac ggtttgctcg
     2761 ctcttcacca ggatagatta gggcattgat agtaacacac gcgtgcaata aacatgccag
     2821 gaaaggctgg gaggagccgt actaataaag cgaaacctga gctaacctta ctggcacggc
     2881 tcttgcttcc ataagaacgt gcgtagcggc agtaagtcgc gaggcgcaca caagggcaaa
     2941 cgtaaacagt ttacaagggg gtaaatgaaa tgactagttg gaatcgcgga cgcgagaacg
     3001 aagaaatcgg cgagcagcag ttgaacgacg gcgacccggg caacaccctg gaaggtgccg
     3061 cgcgcaaggt cggcggcgag atcgagcagg gcctcgacaa cctgggggac accctcaccg
     3121 gcaagcagaa cgacctgcag ggcaaggacc agaactgggg caaccgcgcg ggcgatgcaa
     3181 tcgacgacgc cggagacaac ctgcgcaacg ccagcgacaa cgcaggcgat tggatcgaga
     3241 accgaggcga cgacatccga gacgccacgc gctaacgcga attcttaatg cttaattctt
     3301 aattgaattg agcaaacgga aaggagccac cgcagatagt ggctcctttt tcgttgcctg
     3361 ctggtatcca gggcaattaa gcattaagaa ttaagaattc tcctcagtag cttctgccgt
     3421 agttgggggc ttccttggtg atggttacgt cgtgggggtg gctttcgcgg aggccggagc
     3481 cggagatgcg gatgaagcgg gcctgctcct ggagcgtctt gatgtcgggc gcgccaaggt
     3541 agcccatgcc gctgcgcagg ccaccgacca actggtagag cagcgtgccg agggggcctt
     3601 tgtactgcac gcgggcctcg atgccctccg gcaccagctt gctgatggct tctacttcgt
     3661 gctggccgta gcggtccttg gagaaggaac gctgcttcat cgcgccgagg gaacccatgc
     3721 cccggtactc cttgaagcgc tcgccctggt agaggatgat ctcgccgggg ctttcgtcca
     3781 cgcccgccag caggctgccc agcatgaccg tgtcggcacc tgccgcgatg gccttggcga
     3841 tgtcaccgct gtactgtatg cccccgtcgg caatgaccgg cacccctgcc tcgcggcaca
     3901 ctttgtagca ctcccacacg gccgttatct gcggcatacc cgccccggac actacgcgag
     3961 tcgtgcagat ggaattgtgt acgattgcgt tgttagcgat gaagctgtgg ctgtcgcatt
     4021 ccacctctat atcatacacg ggaactacaa cggtagtagg gctgttgagc tcaagaggct
     4081 tgacgacctg atattcctgc attctgcgtt tttggtgcgt cctgttcagc ctggcgcgga
     4141 atgaaggtct caggttgtac acgcccccgt taaccttgcc ggggccagcc tcaccgtggg
     4201 cctccatgtt gggcaacgaa ccatagatgt ggtgacacag cagagagaat agctcaacaa
     4261 gacgtggtg
//
LOCUS       DASRWZ010000568         9864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_46178,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000568 DASRWZ010000000
VERSION     DASRWZ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46178"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="VFH60_08955"
     CDS             complement(<1..1753)
                     /locus_tag="VFH60_08955"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HET6313951.1"
                     /translation="MRIGVGGNRVSRRTALLLGRVFGVALVLGALVWFAGGALTPRSL
                     GAPMATPTIDGNITVGEYGNHTDGQNAKSSGSQTWYVTWDENNLYVALTNANIFEGAV
                     FYLDKDPLPTPNGGSNANGTLVGQPYDNSGLASLPFRADFVTYFKNGYREYRTANGAG
                     GWTDPPSSYAGGNYADNGATNTRELAIPWSVITGSGRPASFNFLGYVAYSNQFGTGVY
                     AQLPPRNPGGAIGTANTQFHRFYRVNSTASLTGTPPFSLDVATFVVNSTADTGDAGTD
                     GICGEPLGSTICTLREAIQEANAVPLPSSYDTITFNIGTGLQTISPASLLPSINDPVR
                     IDGTTQPGYAGTPIIQLTGTPSNFGLTINTNNSTVMGLVINNFAGAIYIVGGSGNKIQ
                     GNYIGTNAAGTSAVPNSQGIVLEGVTGNVIGGTDPLARNLISGNSTAGVYIFFSTLNT
                     VQGNYIGTNAAGTAALPNHTGVLITASDDNTIGGTTAAARNLISGNTTGVDMDGTSQR
                     NLVQGNYVGTNATGTGAIANTTGISLRAPANTVGGTATGAGNLISGNGTGVFITANGA
                     NATGNLIQGNYIGTTITG"
     gene            complement(2104..2571)
                     /locus_tag="VFH60_08960"
     CDS             complement(2104..2571)
                     /locus_tag="VFH60_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313952.1"
                     /translation="MATNTKQNTARSDNDTNGNNGNGAGERSGNLRDSEMMAHLMDAL
                     EKGTDIGHYGRLTFAMVARHFLDEDEMLRLLGNQEGGEEEARRLLVQVQARDYNPPKR
                     ERILQWQQEQEFPICPNADDPDACNVYRDLRFPDGIYESIEEYYEEKAEAQQS"
     gene            complement(2852..5485)
                     /locus_tag="VFH60_08965"
     CDS             complement(2852..5485)
                     /locus_tag="VFH60_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6313953.1"
                     /translation="MNDFLFLQPQTLWLAPFLVLLLVLSIRLVRGLAPWRRRTAVFFQ
                     ALTVLLLVASLAQPAFSRPQGDMSLVVVLDSSASVSEEGRTQATEFARNVLDTAGPDV
                     TVQFVEVGQRATQLTNEQVQSGEWSVISDTNSLVADPNLRATDLSSGLRLAGSLLKDE
                     GRRRVVVVSDGWETLGRAADEALRLQGRGIDVQVMSLSALGTPEVIVRSLDVDPYARV
                     GDPVRTGLNVFSTNDGPAVLSLSVDGQPLLTQTVTLKTGENHLPVDHRALTAGFHRLD
                     ATVTSQADSATENNSAAASIMVKAQPNVLVLEDRPGEADRLVSTLINNQMAVEVRPPA
                     FIPARLSDLDPYASIVLYNVAATSMTLDQQRTLQEYVRRSGKGLVAIGGQTSFAKGGY
                     QDSVLDEVMPVSSDPGPRPEKGETALILVFDRSSSMDEWMGLSNQDTKFSMAKEAARL
                     SIDALRAGDIFGVLSFDTENLWTIEPVTIHADTDKQALKEKITQIQLGGGTSIYPAVV
                     QAGETILTLKAQSRHLVLMTDGKDYHQGGYDEIIDKLRASDVSLSTIAVGSDSDRDTL
                     IELARKGEGRHYFTERLDNIPKIVFRELDLALKESVIEGTIQPHVQETSPVLRGIAPG
                     SIPQLGGYDITSPKDASVTALTADLGQPLLAHWNYGLGRVLAFTSSVDQAWSKDWLGW
                     SDFDRFWNAAVRWTMQSPLNRQLQPTVSVTTDEAGQPIAHISVESLNDDLGFSDLTDI
                     TAGLRSPSGVVTTTLLSQTGPGRYEADVPVSELGAYEVRLTRSGGEGVSQTETAGFNV
                     PVGEEWAHTGTNDRLLKRLNGGKDYITDPGMALDGTGLTGAVVEYDPLWFYPLAAALV
                     TLLLSVAARRIEFRGRRKRTA"
     gene            complement(5482..7269)
                     /locus_tag="VFH60_08970"
     CDS             complement(5482..7269)
                     /locus_tag="VFH60_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HET6313954.1"
                     /translation="MNLLNPSAAWWLLMVPALIALYMLRPRARRKPVPSLRLWRELPQ
                     VERPTPRLRRPPLSWLLLLQALLLAAGAFALMQPALFAPAARHTVVLLDTSGSMQTLE
                     GSSTRFERARTEAGNVVARLRDTDRITLLGVGPSVTTACSNCTRNDFNRALREMKLSG
                     GRANWPSAVSLAAGLSGQAGANATTTYAISDGAFEALRGDDVPPDMHFVQVGSNVANL
                     AVSVLSARRPPNNSPGYSAYARIENVSTSHANVQVSAVADTVPLTPRSLSIPAGGHAD
                     VTWEVPAGALKLTVGIDAQDALAADNQAVLFLPTNSQNQVIVKSDNPGMYMRAVAGYH
                     GLQPITDTVQSAAETAFTVWEGELPSALPAGGLFLVNPSGGLLPTAGDMENLGSPDTV
                     SDHPLLRDIDLRALFVERARKYEAPAWLEPIVQTADGPLLLAGEQDGRRIAVLAFDPR
                     ESNLPKLAAFPLLMANLADWLYPQSGVGALRPGDAAIVPPGATVTTPGGRKVQASEAG
                     VFADTSEVGIYTVAGANAQDTSRSFAVNMTDTKESNVTPKDHPELERAAVEVAERVTT
                     EDYWSPLAALALILAGGEWLVYCLKRGRM"
     gene            complement(7266..8240)
                     /locus_tag="VFH60_08975"
     CDS             complement(7266..8240)
                     /locus_tag="VFH60_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6313955.1"
                     /translation="MRPAGGIRATLGNLGRRFARRAPGFAPVVETNVVDGDFLHKLDR
                     LSLTLGRDLINGLMGEHLSTRRTSGIEFADYRQYSAGDDLRRVDWNAYARLGTLHVRQ
                     SQAEHDTILYLLVDASPSMDFGNPSKFLSARRLAAGLGYIARSHLDSVVLAAPGAGNA
                     VAPDPFRGRGQSPALFRTMQELSVGQALDFDGILKEWSAQRMGGGAVGRLAVIVSDLL
                     LDGWRDGVRNLVTNGFGVTVLHLLSPEELTPPEDGDFQLEDSETGERLDVHLGAASLQ
                     EYDRRVTAWLDETEAWCQSNAARYVRLQSDWDIERTLLDLLRKRGVTA"
     gene            complement(8237..9232)
                     /locus_tag="VFH60_08980"
     CDS             complement(8237..9232)
                     /locus_tag="VFH60_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6313956.1"
                     /translation="MQAWTPEQAEGLAERVQQIVGTLEHELGSVIVGQKQVVRGVLTA
                     LLAGGHVLLEGVPGLGKTLLVRTLAECLDLTYSRIQFTPDLMPADIVGTDIINETDGM
                     RAFAFQPGPIFAHLVLADEINRATPRTQSAMLEAMQEHVVTVGRTSHPLPEPFFVMAT
                     QNPIDLQGTYPLPEAQLDRFFFKLIVEYPQGDELKEIARRTTGNRPSNARKVMDGPTL
                     ARLQQIVRDIPVAEEVLDYAANLVMATHPETANATPSAKQFVRFGASPRAIQSLILAG
                     KVNAILSGRYNVSYDDLKRVALPSLRHRMVLNVEAQLQNVTADAIITRVLEEVQP"
     gene            complement(9339..>9864)
                     /locus_tag="VFH60_08985"
     CDS             complement(9339..>9864)
                     /locus_tag="VFH60_08985"
                     /inference="COORDINATES: protein motif:HMM:NF025428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HET6313957.1"
                     /translation="EGSHIDVVSEWAEHIDKVPGRRRAMLEQLLREKAGEWPENAEPH
                     NYFSIPYEWTDGYKRFKPLRTVRVALVPTPASWEVPAYLKFGAWNDCPQPEEHVAILK
                     RWHAQYGAQVVGLTHDVLEVGVTRPPNSKLSAFNLAREQYGYCSDLVDQGDGGILDLA
                     ATLLDASVWYFWWD"
BASE COUNT         1625 a         3064 c         3335 g         1840 t
ORIGIN      
        1 tgcccgtgat ggtagtgcct atgtagttgc cctgtatcag gttgcccgtg gcgttggccc
       61 cgttcgccgt gatgaacacg cctgtgccgt tgccggagat gaggttgccc gccccggttg
      121 cggtgccacc caccgtgttt gcgggggcac ggagagatat gccggtggta ttggctatag
      181 cgccggtacc cgttgcgttg gtgccgacgt agttaccttg tactagatta cgttgggaag
      241 tgccgtccat gtccacgccg gtggtgttgc ccgaaatgag gttgcgcgcc gcggccgtgg
      301 tgcccccaat cgtgttgtca tcagaagccg taatcagcac gcctgtatgg ttgggcaagg
      361 cagccgtgcc cgcggcattc gtgcctatgt agttgccctg cactgtgttg agggttgaaa
      421 aaaagatata aacgcctgcg gtgctgttac cggatataag gttgcgcgcc aggggatctg
      481 tgccccctat cacgttgccg gtgacaccct ctagcactat accctgagag ttgggaacgg
      541 ctgaggtccc ggcggcgttg gtgcctatat agttgccctg gatcttgttg ccactgcccc
      601 cgactatata gatggccccg gcgaaattat tgattacaag gcccatgaca gtgctgttgt
      661 tggtgttgat ggtcaaaccg aagttggacg gagtgccggt cagttggatg ataggggtgc
      721 ccgcgtaacc aggctgagtg gtcccgtcta tccgcaccgg gtcgtttatg ctgggcagaa
      781 ggctggccgg gctgatggtc tgcaagccgg tcccaatgtt gaaggtgatg gtatcgtagc
      841 tgctgggcag gggaacggcg ttggcctcct gtatagcctc gcgcaaggtg cagatggtgg
      901 agcctaacgg ctcgccgcag atgccgtccg tgccggcatc ccccgtgtcg gcggtggagt
      961 tgaccacgaa ggtagccacg tccagcgaga agggcggagt gccggtcagc gaggcggtgc
     1021 tgttgactcg gtagaatcgg tggaattgcg tgttggcggt gccgatagcg ccaccagggt
     1081 tacgaggggg cagctgcgcg tagacaccgg tgccgaattg attgctgtaa gcaacatagc
     1141 cgaggaagtt gaacgacgca ggcctgccac tcccggtgat gacggaccag ggtatagcca
     1201 actcgcgggt gttggtcgcg ccgttatcgg cgtagttacc accggcatag ctactggggg
     1261 ggtcggtcca cccgcccgca ccattggcgg tacgatactc gcggtagccg ttcttgaaat
     1321 atgtaacaaa atcggcccta aacggcaagg aagcgaggcc actgttatca tagggctggc
     1381 ctacaagggt gccgttggcg ttggagccgc cgttgggggt gggcagcggg tccttgtcga
     1441 ggtagaagac cgcgccctcg aagatgttgg cgttcgtgag ggccacgtac aggttattct
     1501 cgtcccaggt cacgtaccag gtctgcgagc cgctggactt ggcgttctgc ccgtcggtat
     1561 ggttgccgta ttccccgacg gtgatgttgc cgtcaatggt gggggtggcc atcggcgcgc
     1621 ccagcgagcg aggcgtcaga gcgccccccg cgaaccacac cagggcaccc agtacgagcg
     1681 ccacgccaaa tacacggcca agtaataagg ccgtgcgccg agatacgcgg ttgccgccca
     1741 cgccaattct catcctaccc tcccgaacgt aatgccggca cgcgagtccg gccagcgcca
     1801 cagtaacccg ttatgcgtga cgcgccctca aatcaagctc tatgagcctg gctgcctata
     1861 cctcgtagca gaccccaggc gaacagtgct aagtataaca aatagcaata cccggttgta
     1921 aatacctttg gagacgaaaa cgccaaaacg cgaggggcca cgccaggatt gagggtgact
     1981 gtaacaatca ctaagaagac aacagagccg ccagcctacg ccccacgtag gacatagaat
     2041 acaaaaagga ccaggttgcg caacctggcc ctctcttgcc atacacaagg tgtaagctgc
     2101 tgcctagctc tgctgggctt cggccttctc ctcgtagtac tcctcgatgc tctcgtagat
     2161 accgtcgggg aagcgcaggt cgcggtagac gttgcaggcg tcggggtcgt cggcgttggg
     2221 gcagatgggg aactcctgct cctgctgcca ctggagaatg cgctcgcgct tgggcgggtt
     2281 gtagtcgcgg gcctgcacct gcacgagcag acgccgggcc tcctcctcgc caccctcctg
     2341 gttgcccagc agccgcagca tctcgtcctc gtcgaggaag tggcgggcca ccatcgcgaa
     2401 ggtgagcctg ccgtagtgcc cgatatcggt gcccttctcc agggcgtcca tcaggtgcgc
     2461 catcatctcc gagtcgcgca ggttgccgct ccgctccccc gcgccgttgc cgttgttgcc
     2521 gttggtgtcg ttgtcggacc tggcagtgtt ctgcttggta ttcgttgcca tctcgctctg
     2581 tacctccttc taaaatagaa cacatgttca agtaaattta tgcaagatgc gtgccagcta
     2641 ccaatcactt ccgaggcacc ccctgggcga cccgtaccgg gtcgcgatta gcgatgcaac
     2701 cgcaaaggcg caaagtgcgc aaagttgacg gattgttggt tgcacaaaaa aggaccaggg
     2761 tacatgctgt cgtaccctgg tctttttact tagaacacct gcttagtctc tcagtgtcct
     2821 ttgcgctctt tgcgcctttg cggtttcatc gtcaagccgt tcgcttgcgg cggcctcgga
     2881 actcgatgcg gcgggcggct acgctcagga gcagggttac cagggcggcg gccagggggt
     2941 agaaccagag agggtcgtac tccaccaccg cgcccgtgag gccggtgccg tcgagggcca
     3001 tgccggggtc ggtgatgtag tccttgccgc cattgagacg cttcaggagg cggtcgttgg
     3061 tgcccgtgtg cgcccactcc tcgcctacgg ggacgttgaa gccggccgtt tccgtctgcg
     3121 acaccccctc gccgccggag cgtgtgaggc gtacctcgta agctccaagc tcgctcaccg
     3181 gcacatcggc ttcgtagcgg cccggccccg tctggctcag cagggtggtg gttaccacgc
     3241 cggaggggga gcgcaggccc gccgtgatgt cggtcaggtc cgagaagccc aggtcgtcgt
     3301 tgaggctctc caccgagatg tgggcgatgg gctgccccgc ctcgtccgtg gtgacactca
     3361 ccgtgggttg aagctggcgg ttgagggggc tttgcatcgt ccagcgcacg gcggcgttcc
     3421 agaagcggtc gaaatcgctc caccccagcc agtccttcga ccaggcctgg tccacgctcg
     3481 acgtaaaggc gagcacgcgc cccaggccgt agttccagtg cgcgagcagg ggctggccga
     3541 ggtcggcggt gagggccgtc acgctggcgt ccttgggcga ggtgatgtcg taaccgccaa
     3601 gctgcgggat ggagcctggg gcgataccgc gcagcaccgg gctggtctcc tgcacgtggg
     3661 gctggattgt gccctcaatg acggactcct tgagcgccag gtcaagctcg cggaagacga
     3721 tcttgggtat gttgtcgagc cgctcggtga agtagtgccg cccctcgccc ttgcgcgcaa
     3781 gctcgatcag cgtgtccctg tccgagtcgg agccgaccgc gatggtggag aggcttacgt
     3841 cgctggcccg cagcttgtcg atgatctcgt cgtagccgcc ctggtggtag tccttaccgt
     3901 cggtcatcag caccaggtgg cgcgactgcg ccttgagggt gagtatagtc tcgcccgcct
     3961 gcaccaccgc cgggtagatg ctggtgccgc cgccgagctg tatctgggta atcttttcct
     4021 tcagcgcctg cttgtcggta tcggcgtgta tcgtcaccgg ctcgatggtc cagaggttct
     4081 cggtgtcgaa gctgagcacc ccgaagatgt ccccggcgcg cagggcgtca atcgagaggc
     4141 gggcggcttc cttcgccatg ctgaacttgg tgtcctggtt gctcaggccc atccattcgt
     4201 ccatcgagga ggagcggtcg aacaccagga tgagggccgt ctcgcccttc tccgggcggg
     4261 ggccagggtc ggaggatacc ggcatcacct catccagcac gctgtcctgg tagccgccct
     4321 tggcgaagct ggtctggccg cctatagcca ccaggccctt gccgctgcgc ctcacgtact
     4381 cctgcaaggt gcgctgctgg tccaatgtca tgctggtagc ggccacgttg tacagcacga
     4441 tgctcgcgta ggggtcgagg tcggacaggc gggccgggat gaaggcgggc gggcgcacct
     4501 ccacggccat ctggttgttg atcaaggtgc tcaccagccg gtccgcctcg ccggggcggt
     4561 cctcgagcac cagcacgtta ggctgggcct tgaccatgat gctggcggcg gcgctgttgt
     4621 tctcggtcgc ggaatcggcc tgactggtta cggtcgcatc gaggcggtgg aagccggccg
     4681 tgagggcgcg gtggtccacg ggcaggtggt tctcccccgt cttgagcgtc acggtctgcg
     4741 tcagcagtgg ctgaccgtcc acgctcaggc tgagcacggc ggggccgtcg ttggtgctga
     4801 agacgttcaa gccggtgcgc accgggtcgc ccacgcgggc gtaggggtcc acgtcgagcg
     4861 agcgcacgat cacctcaggg gtgcccaggg cagagaggga catcacctgc acgtcgatgc
     4921 cccggccttg caggcgcaaa gcctcgtcgg cggcgcggcc cagcgtctcc caaccgtcgc
     4981 tcaccacgac cacccgccgc ctgccctcgt ccttcaggag gctacccgcg agccgcaggc
     5041 cggaagatag gtcggtggcc cgcaggttgg ggtccgccac tagcgagttg gtgtcgctga
     5101 tgaccgacca ttcgccgctt tgcacctgct cgttggtaag ctgagtggcc cgctgcccca
     5161 cttccacgaa ctgtacggtg acatccggcc cggcggtgtc gagcacgttg cgggcgaact
     5221 cggtggcctg cgtgcggccc tcctcgctga cgcttgccga ggagtcgagc accacaacca
     5281 ggctcatgtc gccctggggc cgcgaaaagg cgggctgggc cagggaagcc accaggagca
     5341 gcacggtgag cgcctggaag aagaccgccg tgcgccgccg ccagggagcc agcccgcgca
     5401 ccaggcgaat ggagagtacc agcagcaaga ccaggaaggg tgccagccac agtgtttgcg
     5461 gttgtaggaa taggaagtcg ttcacatgcg gcctcgtttc aggcagtaga caagccactc
     5521 gcccccggca agtatgagcg ccagcgcggc caggggcgac cagtagtcct ccgtggtcac
     5581 acgctcggcc acctccacgg cggcccgctc aagctccggg tggtccttcg gagtgacgtt
     5641 agattctttc gtatcggtca tgttcacggc gaacgagcgg gaggtatcct gcgcgttcgc
     5701 cccggccacg gtgtagatgc ccacctcgct ggtgtcggca aatacgcctg cctcgctggc
     5761 ctgcaccttc ctgccgccgg gggtggtcac ggtagcgcct gggggcacaa tggccgcatc
     5821 gcccggacgg agcgcgccca cgcccgactg cgggtagagc cagtcggcca ggttcgccat
     5881 gagcagaggg aaagccgcca gtttgggcag gttagactcg cgggggtcga aggccagcac
     5941 ggctatacgg cggccgtcct gctcccccgc cagcaggagg gggccgtcag cggtctgcac
     6001 gattggctcg agccaggccg gggcctcgta cttgcgggcg cgctccacga acagggcgcg
     6061 caggtcgatg tcgcgcagca gcgggtggtc ggacacggta tcgggcgaac cgaggttctc
     6121 catgtcgccc gccgtgggga gcaggccgcc ggaggggttc accaggaaga ggcccccggc
     6181 gggcagggcg gagggtagct ccccctccca caccgtaaag gcggtctcgg cggcgctctg
     6241 gaccgtatcg gtgatgggtt gcaggccgtg gtagcccgcc accgctcgca tgtacatgcc
     6301 ggggttgtcg gacttgacga tcacctggtt ttgcgagtta gtgggcagga agagcaccgc
     6361 ctggttatcc gcggccaggg cgtcctgggc gtcgataccc acggtgagct tgagggcacc
     6421 ggctggcact tcccaggtca cgtcggcgtg gcccccggcg gggatgctca ggctgcgggg
     6481 cgtgagcggc accgtatcgg ccaccgccga cacctgcacg ttggcatggg aagtagatac
     6541 gttctcaatg cgcgcgtagg ccgagtagcc gggcgagttg ttgggcggcc tgcgggcgct
     6601 cagcacgctt acggccaggt tggcgacgtt gctacccacc tgcacgaaat gcatatccgg
     6661 gggcacgtca tcgccccgta gcgcctcgaa tgcgccgtcc gaaatggcat aggtagtggt
     6721 ggcgttggcc cccgcctgcc ccgacagacc ggcggcgagg gacacggcgg aaggccagtt
     6781 ggcgcgcccc ccgctcagtt tcatctcccg cagggcgcgg ttgaagtcgt tgcgggtgca
     6841 gttggagcag gcggtggtca cgctcggccc cacgcccaac agggttatgc ggtcggtgtc
     6901 gcgcagcctg gctaccacgt tgccggcctc ggtgcgcgcc cgctcgaagc gggtagacga
     6961 gccttccagg gtctgcatgc tgcccgacgt atcgagcagc accacagtat gccgtgcggc
     7021 gggtgcgaag agggcgggct gcatgagggc gaaagccccg gcggccagca gcagggcttg
     7081 cagcagcagc agccaggaga gcggcgggcg gcgcaggcgc ggggtgggac gctccacctg
     7141 gggtagctcg cgccacagcc tcaggctcgg cacgggcttg cggcgggcgc ggggccgcag
     7201 catgtagagg gcgatgaggg cgggcaccat gaggagccac cacgcggcgg aggggttcag
     7261 caggttcaag ccgtcactcc cctcttgcgc agcaggtcca gcagggtgcg ctctatgtcc
     7321 cagtcgcttt gcaggcgcac gtagcgggcg gcgttcgatt ggcaccaggc ctcggtctcg
     7381 tccagccagg cggtcacgcg gcggtcgtac tcctgcaagc tggccgcgcc gagatgcacg
     7441 tccagccgct cgcccgtctc gctgtcctca agctggaaat cgccgtcttc ggggggcgtc
     7501 agctcttcgg ggctgagcag gtgtagcacg gtgacgccga agccgttggt caccaggttg
     7561 cgcacgccgt cgcgccagcc atctagcagc aggtcggaca cgatcaccgc gaggcgaccg
     7621 accgcgcccc cacccatacg ctgggcgctc cactctttca ggatgccgtc gaagtccagc
     7681 gcctgcccaa cgctcagctc ttgcatggtg cggaacagcg cgggcgactg tcccctaccc
     7741 cgaaacgggt cgggggcgac cgcgttgccc gcgccgggtg ccgccaacac cacgctatcc
     7801 aggtgcgacc gggcgatgta gcccagcccg gctgccaggc ggcgggccga gaggaacttg
     7861 ctggggttgc cgaagtccat agaagggctg gcgtccacga gcaggtacaa aatggtgtcg
     7921 tgctcggcct gcgactgccg gacgtgcagg gtgcccaggc gggcgtaagc gttccagtcc
     7981 acgcgccgca ggtcgtcgcc cgcgctgtac tggcggtagt ccgcgaattc gatgcccgag
     8041 gtgcgcctgg tggataggtg ctcgcccatc agcccgttta tgaggtcgcg acccagggtg
     8101 agggagaggc ggtccagctt gtgcaggaag tcgccgtcca ccacgttggt ctccacgacg
     8161 ggtgcgaagc cgggggcgcg ccgggcgaag cggcggccca ggttgcccag ggtcgcccgt
     8221 atgccgcccg ccggtctcaa ggctgtacct cttccagcac gcgggtgatg atcgcatcgg
     8281 cggtgacgtt ctgtagctgc gcctccacgt tgagcaccat gcggtgccgc agagagggca
     8341 gagccacccg cttgaggtcg tcgtacgata cgttgtaccg ccccgacagg atggcgttca
     8401 ccttgcccgc caggatgagc gattggatag cgcgcgggct ggccccgaac cgcacgaact
     8461 gcttggccga gggggtagcg ttggcggtct ccgggtgggt agccatgacg aggttggcgg
     8521 cgtaatcgag cacttcctcg gccaccggga tgtcgcgcac gatctgctgc aagcgcgcca
     8581 gggtggggcc gtccatcacc ttgcgggcgt tggaggggcg attgccggtg gtgcggcgag
     8641 cgatctcctt cagctcgtcg ccctgcgggt actccacgat cagcttgaag aagaagcgat
     8701 ctagctgggc ctccggcagc gggtaggtgc cctgcaagtc tatcgggttc tgggtagcca
     8761 tcacgaagaa cggctcaggc agcgggtggc tggtgcggcc caccgtgacc acgtgctcct
     8821 gcatcgcttc gagcatagcc gactgggttc ggggcgtggc gcggttgatc tcgtcggcca
     8881 gcaccaggtg ggcgaaaatc ggccccggct ggaaggcgaa agcccgcatg ccgtcggtct
     8941 cgttgatgat gtcggtgccc acgatgtcgg cgggcatcag gtcgggggtg aactggatgc
     9001 ggctgtaggt gaggtcgagg cactcggcca gggtgcgcac cagcagggtc ttgcccaggc
     9061 ccggcacgcc ctctagcagc acgtgcccgc ccgcgagcaa agcggtgagc accccgcgca
     9121 ccacctgctt ctgccccacg atcacgctac caagctcgtg ctccagcgtg cccacaatct
     9181 gctgcacgcg ttccgccagt ccctcagcct gctcaggtgt ccaggcttgc attgtattgc
     9241 tcaatctagt ccccttctag gaattttaga ttttagattt tggattttgg attacgcgta
     9301 gtcgtccgta atccaaaatc caaaatctaa aatgcatctc agtcccacca gaagtaccat
     9361 acactggcat cgagcagggt tgccgccagg tccagtatgc cgccgtcgcc ctggtctacg
     9421 aggtcggagc agtagccgta ttgttcgcgt gccaggttga acgcgcttag cttgctgttt
     9481 ggcgggcgcg tcacgccgac ttccagaacg tcatgtgtca ggcccactac ttgcgcgccg
     9541 tattgtgcgt gccagcgttt tagtatggcg acatgttcct ccggctgcgg gcagtcgttc
     9601 cacgcgccga acttcaggta agcgggcact tcccagcttg caggagtggg cacaagggcc
     9661 acgcgcacgg tgcgtagcgg cttgaacctt ttgtagccgt ccgtccactc gtatggaatg
     9721 gaaaagtagt tatgcggctc ggcgttctcc ggccactcac ccgctttttc tcgcagtagc
     9781 tgctctaaca tcgccctgcg ccgacctggt actttatcta tgtgttcggc ccactccgac
     9841 accacatcga tgtggctacc ctca
//
LOCUS       DASRWZ010000569         6040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_390299, whole genome shotgun sequence.
ACCESSION   DASRWZ010000569 DASRWZ010000000
VERSION     DASRWZ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390299"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFH60_08990"
     CDS             <1..294
                     /locus_tag="VFH60_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313958.1"
                     /translation="DNLGAGGGGAAGAVGGGIVGGAIGGPVGAVAGAAIGGGVGAGAG
                     ENAEEDLEGGDRTGYNTGTTGTGYTGGTGTGTSGYGSTGSTGTGYDDNTNRTS"
     gene            808..1920
                     /gene="pyrB"
                     /locus_tag="VFH60_08995"
     CDS             808..1920
                     /gene="pyrB"
                     /locus_tag="VFH60_08995"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HET6313959.1"
                     /translation="MAATYSAAEGALRSLAYQHILTVEQFTPETLEALFALAQQMDRR
                     VADGRVEPLMSSKIVANVFYEASTRTDLSFQAATQRLGGRVISTAGGVHYSSVTKGES
                     LHDTIKTIACYSDAIVLRHPQRGAAKEAAAASDALRLAMHSPAPIINAGDGIGEHPTQ
                     ALLDLYTIKDRFGSDIDGLTIGFVGDLRNGRTVHSLIKLLAIDRCHVNVVCIAPDSLS
                     MPPEYIEFARSREMPVTETQDLQEAIQHVDVLYVTRVQRERFVAQQMEDLSAKIYGLP
                     QEALDAARRDTLRPLADLNAAQEYLAARDTYVVDPMMLSQARKEMIVMHPLPRVNEIP
                     EQVDSDDRAYYFRQVQNGLYIRMALLASVLDCMPER"
     gene            2116..4335
                     /locus_tag="VFH60_09000"
     CDS             2116..4335
                     /locus_tag="VFH60_09000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET6313960.1"
                     /translation="MAADDMTNTSEATTRKVEIEDLYRFRLLSDPQVSPDGRAVAYVQ
                     TRLRKKKNDYASNIWLLPLDGEGEPARFTGSDKRDYYPRWSPDGSQLAFISTRSGKPQ
                     AWVIRAGGGEARQLTRSKHPVTDLVWSPDGKWIVFVSPVDNEEDKRRAEEGKKDKSGK
                     SASSDSEAREPGYAGEEGLPGGILPAGEWEEDSEEDAGAEDKGDHAKAITWMHFKADG
                     AGLLERRQHLFIVPSKGGTARQLTEGDWNAATPRWSPDGRQLAYIANQEADADYFNIQ
                     DIFVLPIDEEGAAGEQRRVTDHDCAIGNMDWLRDGSGFVVFAHRRIQEAAFSTNMQVW
                     TISLTGMKNRLTEGFDRSAANIVNSDLRSAAGELRPRLSADGSTAYFMVTNGGACQVF
                     SVPTAGGEVTQVVGGNREVLNFSVAPGGLVFVATDWNTPNDLYFTATGGEQRRLTDVN
                     ADILPQLQISEPEQFELETAPGVSVEGWILKPPGFDPAQKYPLIVEIHGGPHTAYGYP
                     YFHEFQVLAAKGYVVLYTNPRGSQGYGQEFSDAIISDWGGVDYEDIMACVDHVVGLGY
                     VDEERLGVTGGSYGGYMTGWIIGHTQRFKAAVASRMVSNLHSAWGSGDFTWRLWNFEM
                     EGDPYKRTELYIERSPVTYAHDMRTPLLITHAADDLRCNIEQADQMYTALKVLKRDVK
                     MVRFPSGGHDISRSGKPTLRVERLQHIAGWFDKYLQPDSESSEEKQSRRRNINHPKF"
     gene            complement(4576..>6040)
                     /locus_tag="VFH60_09005"
     CDS             complement(4576..>6040)
                     /locus_tag="VFH60_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313961.1"
                     /translation="YWVDVLVAGAPARVMLQLFERRTLTYNPANPPQWQVEMANVGRA
                     YYDWRYGGTAPEPAISAEVLPDGVQLRGWNWPPNVEVSARIELGGAETPLVGPLSLQS
                     DASGRFGALAPMNTEVEGALLAGANLRIVAYVGDRQTALPLAVELPSGSPAVEGMLTG
                     VVPTQAGYLLLVRDHAGREWHVDASFEAVLSYSEGEETQDATLATGDHVRVEGRILGG
                     KIRARSLRLMSVSRTGARLGYDFEAGGGLIRASGTNWPASGNIAFSVRPFAGEGGVQL
                     GTARADSRGNVAASFPVPPAPQADRPLWLFAQVVEQNSLMAQVAVLYRPAWEANGPLG
                     LILTADSGEQMGGPGGYCHVGQCAELLGVPVPRAALPVREGNVLGLRSQVGPNLDLGI
                     TPQRFTAQLYAYPPEPANEGASIGGTFYFSPKSLPVFSTGEVPGRPFSVSLPSDLPPG
                     KYLLIAAVVWSEGPGPREDATYGFALEVPPFPRPIPR"
BASE COUNT         1221 a         1947 c         1895 g          977 t
ORIGIN      
        1 gacaacctgg gtgccggcgg cggtggcgct gctggcgcgg tcggtggcgg catcgtgggt
       61 ggcgcgatag gcggccccgt aggtgctgtc gcaggcgcgg cgataggcgg cggcgtgggt
      121 gccggtgccg gtgagaacgc agaggaagac ctcgaaggcg gcgaccggac aggctacaac
      181 acgggcacca cgggtaccgg ttacaccggc ggtaccggca ctggtacttc cggctacggc
      241 agcaccggct ccaccggtac gggctacgac gacaacacca accggaccag ctaacacacc
      301 ccaaaaacat cggggcactg acttgaccag gcgtggcctg gcaagcagac agaacgaaca
      361 gggacttgcg gtgcaagtcc ctgttcgtgt taattcccgg tttcgagcag aactgccaaa
      421 tgagtgaact gcccgaacaa aacttatgat atcctcgtga gccagcttgg cacatttcgt
      481 tgccgagaca attacttgcc agcctcaccc tgattgagcg aggacgcggc ctgtgggaac
      541 gccctgtggc taaagccacg ggctgagaag aggcaaagtc ccctacgggg actgaggaac
      601 gtgagggcag tctccgacgg agacttcgtc tctggtcagc ccgccaattc attggcaggg
      661 cacacccaca gggctacctc agtgccgaga aggacaacac cccttacctt ttccactcct
      721 tttgcccgca tgatgggcgc atgctataat ccatccggct ttcagcccgc gcgacaccaa
      781 caacggcagc acgttaggag atacagaatg gcggctactt actcggcagc ggaaggggcg
      841 ctcaggagcc tggcgtacca gcacatcctc acggtggagc agtttacacc tgagactttg
      901 gaggcgttat tcgccctcgc acagcagatg gaccggcgcg tggccgacgg gcgcgtggag
      961 ccgctcatgt cgagcaagat cgtggcgaac gtcttctacg aggcgtccac ccgcaccgac
     1021 ctctcgttcc aggccgctac ccagaggctc ggcgggcggg tcatcagcac ggcgggcggg
     1081 gtgcactact cgtcggtgac gaagggcgag agcctgcacg acacgatcaa gacgatagcc
     1141 tgctactccg atgctatcgt gcttcgccac ccccagcgcg gggccgcgaa ggaagccgcc
     1201 gccgcctccg acgccctgcg cctcgccatg cacagccccg cgcccatcat caacgccggg
     1261 gacggcatag gcgagcaccc cacgcaggcc ctgctcgacc tctataccat aaaagaccgc
     1321 ttcggcagcg acatcgatgg gctgacgata ggcttcgtgg gcgaccttcg caacggccgc
     1381 accgtccact cgctcatcaa gctcctggcg atagaccgct gccacgtcaa cgtggtgtgc
     1441 atcgccccgg acagcctctc catgccgccg gagtacatcg agttcgcccg ctcgcgcgag
     1501 atgcccgtca ccgagacgca ggacctgcaa gaggcgatac agcacgtgga cgtgctctac
     1561 gtcaccaggg tgcagcggga gcgtttcgtg gcgcagcaaa tggaggacct gtcggccaag
     1621 atctacggcc tgccccagga agccctcgac gctgcccgcc gggacaccct ccgcccctta
     1681 gctgacctca acgccgccca ggaatacctt gccgcacgcg atacctacgt ggtggacccg
     1741 atgatgctca gccaggcccg caaggagatg atcgtcatgc acccactccc gcgcgtgaac
     1801 gaaatccccg aacaagtgga cagcgacgac cgcgcctact acttccgcca ggtccagaac
     1861 ggcctctaca tacgcatggc cctcctcgcc agcgtgctcg actgcatgcc ggagcgataa
     1921 gtgatgagtg acgagtgacg agtgatgagg actgaggact gagtaaagga tgcagaaagg
     1981 cggctagcgt tggcactagc cgctttttga tgactgtgcg gcgcaaggtc cactcagtcc
     2041 tcagtcctca gcgctcatca ctgagataaa tgtcttatgt tatactgtgc cattctacgc
     2101 taaaggagtg tagctttggc tgcagacgac atgacaaaca cgagcgaggc gaccacgcgg
     2161 aaggtcgaga tcgaggacct gtatcgcttc aggttgcttt ccgatccgca agtctccccg
     2221 gacggtaggg cggtcgctta cgtgcagacc cgcctacgca agaagaagaa cgattacgcc
     2281 tccaatatct ggctgctccc gctggacggc gagggggagc ctgccaggtt cacggggagc
     2341 gacaagcgcg attactatcc ccgctggtcg ccggacggct cacagttggc gttcatctcc
     2401 acacggtcgg gcaagccgca ggcctgggtg atacgcgcag gtggcggtga ggcgcgacaa
     2461 ctcacgcgga gcaagcaccc tgtgacggac ctcgtatggt cgcccgacgg taagtggatc
     2521 gtctttgtgt cgccggtgga caacgaggag gacaagcgcc gggctgaaga gggcaagaag
     2581 gacaagtcgg ggaagagcgc cagcagcgac agcgaggcca gagagccagg ctacgcgggc
     2641 gaagaggggc tgccaggcgg tatcctcccg gcgggcgagt gggaggagga cagcgaagaa
     2701 gacgcgggcg cggaggacaa gggcgaccac gccaaagcga tcacctggat gcacttcaag
     2761 gccgacgggg cgggtctgct agagaggcgg cagcacctgt tcatcgtgcc gagtaagggc
     2821 ggcacggcca ggcagcttac cgagggcgac tggaacgccg ccacgccgcg ctggtcgcct
     2881 gacggcaggc agcttgccta catcgccaac caggaggccg acgccgacta cttcaacatc
     2941 caggacatct tcgtgctgcc catcgacgag gagggagcgg cgggcgagca gcggcgcgtg
     3001 acggaccacg attgcgctat cggcaacatg gactggctgc gcgacggcag cggcttcgtg
     3061 gtgttcgccc acagacgcat ccaggaagcc gccttctcca caaacatgca ggtctggacc
     3121 atctctctca ccggcatgaa gaaccggctg accgagggct tcgaccgctc ggcggccaac
     3181 atcgtcaact ccgacctgcg ctccgccgcc ggggagctac gcccacgact cagcgcggac
     3241 ggctcgactg cctacttcat ggtcaccaac ggcggggcgt gccaggtgtt ttcggtaccc
     3301 acggcggggg gcgaggtgac gcaggtggtg ggcggcaacc gggaggtgct caacttctcg
     3361 gtagcccccg gcggtctcgt gttcgtggcg acggactgga acacgcccaa cgacctctat
     3421 ttcacggcca cgggcggcga gcagaggcgg ctcaccgacg taaacgccga catcctcccg
     3481 cagctacaga taagcgagcc ggagcagttc gagttggaga cagcaccggg cgtgagcgta
     3541 gagggctgga tactaaagcc gcccggcttc gacccggcgc agaagtaccc tctcatcgtg
     3601 gaaatccacg gcggcccgca caccgcctat ggctatccct acttccacga gttccaggtg
     3661 ctggcggcca agggatatgt cgtgctctac accaacccaa ggggcagcca gggctacggc
     3721 caggagttct ccgacgctat catcagcgat tggggcggtg tggactacga ggatatcatg
     3781 gcctgcgtgg accacgtcgt cggcctcggc tacgttgacg aggagagact gggtgtgacg
     3841 ggcggctcct acggcggcta catgaccggc tggattatag ggcacacgca gcgcttcaag
     3901 gcggcggtgg cgagccgcat ggtgtccaat ctacacagcg cctgggggtc gggcgatttc
     3961 acatggcggc tgtggaactt cgagatggaa ggcgacccgt acaagcgcac ggagctgtac
     4021 atcgagcgct cgcccgtcac ctacgcccac gatatgcgca ctcccctgct catcacccac
     4081 gccgccgacg acctgcgctg caacatcgag caggccgacc agatgtacac ggccctcaag
     4141 gtgctcaagc gcgacgtgaa gatggtgcgc ttcccctccg gcgggcacga catctcccgc
     4201 agtggcaagc ccaccctccg cgtcgagcgg ctacagcaca tcgccggttg gttcgacaag
     4261 tacctccagc cggacagcga gtccagcgag gagaaacagt cgcggagacg aaacataaat
     4321 catcccaaat tttgagcgtg gcggcgaaag ttacatggaa agagacgtaa acgccaagac
     4381 gccaagacgc caaggaccac gccaagatta ggatggtgtg gtctatgcaa aaggaccagg
     4441 atacgccatc atgtaccctg gtcctctttc tatcctctaa acctcaacta tcttggcgtt
     4501 gtccttggcg tcttggcgtt tacgtcttcg actggtactg ccgtcaaggt gttacaactt
     4561 tcggaatgat ccatgtcacc tgggtatggg ccgtggaaat ggtggcactt ccagggcgaa
     4621 gccataagtc gcatcctcgc gcggccccgg cccttccgac cacacgacag ccgcaatcag
     4681 caggtacttc cctgggggca ggtctgaggg cagggacacg ctgaacggcc tgccgggcac
     4741 ctccccggtg gagaagaccg gcagcgattt ggggctgaag tagaaggtgc cgcctatgct
     4801 tgcgccctca tttgcaggct caggcggata agcgtagagt tgagcggtga accgttgggg
     4861 tgtaataccg aggtcgaggt tcgggcccac ctgggagcgc agcccaagca cgttgccctc
     4921 gcgcaccggc aaggcggcac ggggcaccgg cacccccaat agctccgcgc actgcccgac
     4981 atgacaatag ccgccagggc cgcccatctg ctctccgctg tcggcggtca agatcagacc
     5041 tagcggcccg ttcgcctccc aggcaggcct gtatagcacc gccacctgtg ccataaggct
     5101 attttgctct acaacctgcg cgaaaagcca caaaggccgg tccgcctgtg gggcaggagg
     5161 caccgggaac gaggcggcaa cgtttcctct gctgtcggcc cgcgccgtgc caagctgcac
     5221 tccaccttcg cctgcgaacg ggcggacaga gaacgctata ttgcccgagg cgggccaatt
     5281 ggtgccgctc gctcgaataa ggcccccgcc agcctcgaaa tcgtagccga ggcgggcacc
     5341 ggtccgcgat accgacatga gacgcagcga ccttgcgcgg atcttgcccc caaggatgcg
     5401 gccttcaacc cgcacgtgat cgccagtcgc cagagttgcg tcctgcgttt cttctccctc
     5461 gctgtaactc aatacggctt caaaggaggc atctacgtgc cactccctac cggcgtggtc
     5521 cctcaccagg agcaaatacc cggcttgcgt tgggaccacg cctgtcagca tgccctcgac
     5581 agccgggctg ccggagggaa gttctaccgc cagtggaagc gccgtctgcc tgtcgcccac
     5641 atacgcgact atccgcagat tggcacccgc cagcaaggcc ccctctacct ccgtattcat
     5701 cggggcgagg gcaccgaacc tgccggaagc atcgctctga agggacagcg gcccgaccag
     5761 gggcgtttcg gctcccccaa gttcgatgcg ggcgctcacc tcgacattcg ggggccaatt
     5821 ccagccgcgc agttgcaccc catccggcag cacttccgcc gagatggccg gttccggcgc
     5881 tgtgccgccg tagcgccagt catagtatgc gcggcccacg tttgccatct cgacctgcca
     5941 ctgcggaggg ttggcggggt tgtaggtgag cgtcctgcgc tcgaacagtt gcagcatgac
     6001 cctggcgggc gcgcccgcta ccagcacgtc cacccagtac
//
LOCUS       DASRWZ010000570         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1280856, whole genome shotgun sequence.
ACCESSION   DASRWZ010000570 DASRWZ010000000
VERSION     DASRWZ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280856"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..>2329
                     /locus_tag="VFH60_09010"
     CDS             50..>2329
                     /locus_tag="VFH60_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313962.1"
                     /translation="MLTSSIIIVRSRARWLVLAGLMLALITVGGSLVADPAGRSLAAE
                     PPGGRLTDSVRTLNWNGPDYPAGTTVQDRALCNSTGLCDEYELYVQLPSSYTATQQVS
                     VTVVITWENEVDDYDLYIFDPNGNTAGSSNGFQGGREQATLICPQLNVTYTVQVVPYL
                     VTSAPTGTYNGEATLDTSPGTACNPSVPVADPPRESGGLVFNTSTVVDLQRQVGEPDI
                     VFGQNGDIYASGPWGTSTNQSFAWKSVDNGLTYNTISRIRPDVGPGGGDTTTETDDQD
                     FVYFTDLEGLLNLAAARSTDGGNTWTRNSIAAYETAVDRQWMAIDNGLTNAASDNTQF
                     FTWRQAALGSFVSYSTDGQIFLPAQANPADPINNGAPCGDFIFDPTDRYLYLPCGRAN
                     QVEVARAHVDPGQRTGLVFTSTMAGNAGGETSELFPTLDVDEAGNVYVAYVDTADHNV
                     YLVYSTDNAQTWSDPVRLNGNEARTAVFPWLDAGADGRVAVAYIATDSRGPNNNGDPN
                     DFPSWFNDKIGSTTVKWHAYVNYVTGANTSNPTIYQDRVTEHPMNYGQVCTSGTLCLA
                     TGGDRTMADFLTLQHDHDGALSVIYNDTTNQHHGASVMEARQLAGPSLFGTTLSNVAA
                     TNPVTDTLGDAQYPHFSPTGPGPNYDALDIEGVGIMSDATNLTVTMTISDLNATRVLP
                     GATSVLWLTRWQAKALGDYGEESYRIFYAGARSTGGGAPAFFYGSTQAVEGSPVGCTQ
                     TTPQTCKLLQYPSVPPLVNS"
BASE COUNT          475 a          789 c          690 g          375 t
ORIGIN      
        1 catgtcggta agagtttaca ggattgtatc aggagcagga ggaaagccca tgttaacttc
       61 ttcaattatt attgtcaggt cccgcgctcg ctggcttgtg cttgcgggcc tgatgctcgc
      121 gctcatcacc gtcggcggca gcctggtagc ggacccggca ggacgctctc tggcggcgga
      181 accgcccggc ggcaggctca ccgacagcgt gcgcaccctc aactggaacg gccccgatta
      241 cccggcgggc accaccgtgc aggacagggc attgtgcaac tcgacgggct tgtgcgatga
      301 atacgagctt tacgtgcagt tgcccagcag ctacacggct acccagcagg tcagcgtcac
      361 ggtcgtaata acctgggaga acgaggttga cgattacgac ctctacattt tcgacccgaa
      421 tggcaatacg gcaggtagct ctaacggctt ccagggaggc cgggagcagg ccacgctgat
      481 ttgcccgcag ctaaacgtca cctatactgt gcaggtggtg ccctatctcg tcacgagcgc
      541 gcctacgggc acctacaacg gcgaggccac gctcgacacg tcgccgggta ccgcgtgcaa
      601 ccccagcgtg ccggtggcag acccacctcg cgagtcgggc gggctggtat tcaacacgag
      661 cacggttgtg gacctacaga ggcaggtggg cgagcctgac atcgtcttcg gccagaacgg
      721 cgacatctac gccagtggcc cctggggcac gtccacgaac caatccttcg cctggaaatc
      781 ggttgacaac ggcctcacct acaacaccat cagcagaatc aggcccgatg tcggccccgg
      841 aggcggcgat accaccaccg aaacggacga ccaggacttc gtctacttca ccgaccttga
      901 gggattgctc aatctggcgg cggcgcgctc caccgatggg ggcaatacct ggacgcgcaa
      961 ctcaatcgca gcctatgaga cggctgtgga ccgccagtgg atggcgattg acaacggcct
     1021 cacgaatgcc gcgagcgaca acacccagtt cttcacctgg cggcaggcgg ccctcggttc
     1081 gttcgtgtcg tactccaccg acggccagat attcctaccc gcgcaggcca atcccgcgga
     1141 ccccataaat aacggcgcgc cctgcggcga ctttatattc gaccccaccg accgttacct
     1201 ctacctgcca tgcggcaggg ccaaccaggt tgaggtagcg agagcgcatg ttgatccagg
     1261 gcagcgtacc ggccttgtct tcaccagcac gatggcgggc aacgcgggcg gcgagactag
     1321 cgagctattc cccacgctcg acgtggacga ggcgggcaac gtctacgtgg cctacgtgga
     1381 tacggcggac cacaacgtat acctggtcta ctccaccgac aatgcgcaga cgtggagcga
     1441 ccctgtgcgc cttaacggta acgaggcgcg gacggcggtc ttcccctggc tagacgcggg
     1501 ggcagacggg cgcgtggctg tggcctacat cgccaccgac agccgagggc cgaacaacaa
     1561 cggcgacccc aacgacttcc caagctggtt caacgacaag ataggctcca ccaccgtcaa
     1621 gtggcatgcc tacgtgaact atgtaacggg cgcgaacacc tcaaacccca ccatctacca
     1681 ggaccgcgtg accgagcacc cgatgaacta cgggcaggtc tgcacgtcgg gcaccctctg
     1741 cttagccaca gggggagacc gcacgatggc ggacttcctg accttgcagc acgaccacga
     1801 cggcgcgctc agcgtcatct acaacgacac caccaaccag catcacggcg catcggtcat
     1861 ggaagcgcgc cagcttgccg ggccttccct cttcggcact accctgtcga acgtcgcggc
     1921 caccaacccg gtgaccgata cgctgggcga cgcgcagtat ccccacttca gccccaccgg
     1981 ccccggcccc aactacgatg cgctcgacat agagggcgtg ggcatcatgt cggacgccac
     2041 taacctcacc gtcacgatga ccatctccga cctgaacgcc accagggtgc tgcccggcgc
     2101 tacctcggtg ctgtggctga cgcgctggca ggcgaaggcg ctgggcgact acggcgagga
     2161 gagctaccgc atcttctacg cgggcgcgag gtccaccggg ggtggcgcgc cggccttctt
     2221 ctacggtagc acgcaagcgg tagaaggctc gccggtaggc tgcacccaga ccaccccgca
     2281 gacctgcaag ctgctgcaat acccatcggt gcctccgctt gtcaactcc
//
LOCUS       DASRWZ010000571         1959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_458193, whole genome shotgun sequence.
ACCESSION   DASRWZ010000571 DASRWZ010000000
VERSION     DASRWZ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458193"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VFH60_09015"
     CDS             <1..929
                     /locus_tag="VFH60_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6313963.1"
                     /translation="TAKHDIYQVIGRAAPTEATVLISGETGTGKELVANVIHNYSSYR
                     HGPLVKVNCAALPETLLESELFGHEKGAFTNAITQRKGRFEMAHKGSIFLDEIGEMTL
                     STQRKLLRVLQEREFERVGGSIPIKVDVRVIAATNRNLRAEVDSGRFREDLYYRLDVV
                     RISMPPLRERKEDIPLLVEHFLDKHRYSQTSPPARISEEAMQVLVDHDWPGNVRELEN
                     TVQRAVVMSQAGVVTSHHILLSNLGDRQIVEIGRMVKEATPLHEILGQIEKMALTEAM
                     QQAKGDRSEAARLLGLERPTFYEKLKEYGLSS"
     gene            1375..1875
                     /locus_tag="VFH60_09020"
     CDS             1375..1875
                     /locus_tag="VFH60_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010330823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET6313964.1"
                     /translation="MTLPTHTYLDERGVAYQRLRFPATTEKGAANVAHALGYRERQMV
                     KTLIFEIDTGEKVLVMLGGDQNAISGHLKKALGSRNIRLASPEVVLDTTGYVIGSIPP
                     FHWQPEGFRSFIDAALMQEPVLGVGAGRWGEEIIITPDLLVQVSRAQVVNLTDREQPV
                     QPEPQA"
BASE COUNT          429 a          655 c          566 g          309 t
ORIGIN      
        1 ctacggctaa gcacgacatc taccaggtga taggccgcgc cgcccccacc gaggctaccg
       61 tgctgattag cggcgaaacc ggcacaggca aggagcttgt ggctaacgta atccacaact
      121 actccagcta ccggcacggc ccgctcgtca aggtgaactg cgccgccctc cccgaaactc
      181 tactggagag cgagcttttc ggtcacgaaa aaggggcctt caccaacgcc attacccagc
      241 gcaaaggccg ctttgagatg gcccacaaag gctctatctt cctggatgaa atcggtgaga
      301 tgacgctgag cacccagcgc aagctgctgc gcgtgctcca ggagcgcgag ttcgagcggg
      361 tgggcggctc catccctatc aaggtggacg tgcgcgtgat cgccgccaca aaccgcaacc
      421 tgcgcgccga ggtagactcg ggccgcttcc gcgaggacct ctattaccgc ctcgacgtgg
      481 tgcgcatcag catgccgccc ctgcgcgagc gcaaggaaga catccccttg ctcgtggagc
      541 acttcctcga caagcaccgc tacagccaga cctcacctcc cgcccgcatc tccgaagagg
      601 ccatgcaggt gctggtagac cacgactggc ctggcaacgt gcgcgagctt gagaacacgg
      661 tgcagcgcgc ggtggttatg tcgcaggccg gcgtggtaac cagccaccac atcctgctct
      721 cgaaccttgg agacaggcag atagtggaga tcgggcgtat ggtgaaggaa gccaccccgc
      781 tgcacgaaat cctgggccag atagagaaga tggccctcac ggaagccatg cagcaagcca
      841 agggcgaccg cagcgaggcc gcccgcctcc tcggcctgga acgccccacc ttctacgaaa
      901 agctgaagga atacggcctc tcctcctagc cccagcctcc cccagccctt ccatagcagc
      961 ccagtcccga ccgccgctag ccctcacact accggcggtg cctatttccg taagcggcaa
     1021 gagctacgcc gcgcagtacc aaataccagc ttgtcatcct gagcgcagcg aaggatcagc
     1081 cgcccgttct tggtcacccc ttcgacggcc accaaagccg cgcatttgtc atttcgagcg
     1141 tagcgagaaa tctcgtcctc cagcacccaa agctacaccg ctctcacctc gaaaggcgcg
     1201 cacccaccag catgcccgag gaaaggggca acagaccggt agaggacgag atttctcgct
     1261 acgctcgaaa tgacaaagcc gggactgcta cgccgacaac gcaacacggt ggcgtgatgg
     1321 ctgcttaaag ctcatgcgag ccgagttgct catcgaggac aaatggagca ccaagtgaca
     1381 ctacccaccc atacctacct ggacgaacgc ggcgtcgcct accagcgcct cagattcccg
     1441 gcgaccaccg agaaaggtgc cgccaacgtc gcccacgcgc tcggctacag ggagcgccag
     1501 atggtcaaga cccttatctt cgagatagac acgggggaga aggtgctggt tatgctcgga
     1561 ggcgaccaga acgctatatc tgggcacttg aagaaggcgc tgggttcgcg taacatacgc
     1621 cttgccagcc cggaggtcgt gctggacacc accggctacg taatcggctc gataccgcct
     1681 ttccactggc agcctgaggg tttccggtcc ttcatcgacg cggccctcat gcaggagccg
     1741 gtgcttgggg taggggcggg ccggtggggc gaggagatca tcatcacgcc cgacctgctg
     1801 gtgcaagtca gccgcgccca ggtagtcaat ctcaccgaca gagagcagcc ggtgcagccg
     1861 gagccgcagg cgtaacattg cccacctcgc cctgtgatgg ccctgtggct aaagccacgg
     1921 gcttacaaga ggcaaagtcc ccttcgggga ctgaggaac
//
LOCUS       DASRWZ010000572         1502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_46397,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000572 DASRWZ010000000
VERSION     DASRWZ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46397"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..>1502
                     /locus_tag="VFH60_09025"
     CDS             247..>1502
                     /locus_tag="VFH60_09025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313965.1"
                     /translation="MPYRILTIAPTSFFSDYGAHVRIVEEIWHLQARGHSVVVCTYHT
                     GNNVPGLDIRRSVDVPWKRGVMVGSSRHKLYFDVALAATVQRTARSFKPDIVHSHIHE
                     GALLGWTVRQLRGVPLVFDYQGSLLAEMLDHHFVRPSSPFLPALRLMERRIDRSADAI
                     ITSSYNAEQGLRERIGDYSERISTLPDAVNTQTFSPPSNGMERSATLALKHSLGIPTE
                     RKVVVYLGLLAHYQGTDNLLEAAAIIKNEWGRHDIHFLVMGFPGVETYSSQAERLGLN
                     HHISFPGRIPYPDAPRYLALGDVAVAPKLSLTEGAGKIGNYMAMGLPTVAFDTPVSRE
                     YLGPLGVYAERGNSRDLAAKLCEVLDNPGTYREVGKQLRQQAIANLSWDAAIDHIEAV
                     YEQAKVRRAGQHKASAAGRKERRHEA"
BASE COUNT          290 a          499 c          459 g          254 t
ORIGIN      
        1 tgggtagaca agagtctaca acgtacaaga tactggcggc aaggggaaca ctgttaccca
       61 aataggaaga gtcaagaacg ggcggctgat ccttcgctac gctcaggatg acaaggtctg
      121 cctttggtcc tgctcgttgc ggcacgtgct catcgcacca gcgcgagtat cttccacagt
      181 ttgacacccc caacaaccca cgcctataat gacagctaga gcactaaaca ctcccgcgag
      241 attttattgc cttatcgcat acttacaata gctcctacat cgttcttctc ggactacggg
      301 gcgcacgtgc gcatcgtgga ggagatttgg cacctccagg cgcggggcca ctccgtggtc
      361 gtctgcacct atcacaccgg caacaacgtg cccggactcg acatccgccg ctcggttgac
      421 gtgccctgga agcggggcgt gatggtcggg tcgtcgcggc acaagctcta cttcgacgtg
      481 gccctcgccg ccaccgtgca acgcaccgcc cgcagcttca agcccgatat cgtgcactcc
      541 cacatccacg agggcgcgct cctgggttgg acggtgcggc agttgcgcgg cgtgcccctg
      601 gtgttcgact accagggcag cctcctggcc gagatgctgg accaccactt cgtgcgcccc
      661 agctcgcctt tcctgcccgc gctacgcctg atggagcgtc gcatcgaccg gagcgcggac
      721 gcgattatca cctcctccta caacgccgaa cagggactgc gggagcgcat aggggactac
      781 tcggagcgca tttcgacgct gcccgacgcg gtgaacacgc agaccttctc ccctccctcc
      841 aacggtatgg agcgtagcgc gacgctggcc ctcaagcact cgctgggcat ccccaccgag
      901 cgcaaggtgg tggtctacct ggggctgctg gcgcactacc agggcaccga caacctgctg
      961 gaagccgccg ccatcatcaa gaacgagtgg ggcaggcacg acatacattt cctggtcatg
     1021 ggcttccccg gcgtggagac gtatagcagc caggcggagc ggctgggcct taaccaccac
     1081 atcagcttcc caggccgcat cccctacccc gacgcccctc gctacctggc gctgggtgac
     1141 gtggcggtgg cccccaagct gtcgctgacc gagggggccg gcaagattgg caactacatg
     1201 gcgatgggcc tgcccacggt agcgttcgat acgccggtga gccgcgagta cctcggcccg
     1261 ctgggggttt acgccgagcg cggtaactcg cgcgatcttg ccgcaaagct gtgcgaggtg
     1321 ctggacaacc ccggcaccta ccgtgaagta ggcaagcagc tacgccaaca ggctatagcc
     1381 aacctctcct gggacgcggc gattgaccac atcgaggcgg tgtacgagca ggctaaggtg
     1441 cgtcgtgcgg ggcagcacaa ggcgagtgcg gccgggcgca aggagcggcg gcacgaagcg
     1501 ca
//
LOCUS       DASRWZ010000573         1756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_937881, whole genome shotgun sequence.
ACCESSION   DASRWZ010000573 DASRWZ010000000
VERSION     DASRWZ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937881"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..1604)
                     /locus_tag="VFH60_09030"
     CDS             complement(306..1604)
                     /locus_tag="VFH60_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6313966.1"
                     /translation="MKDQGSSMPCALSQRYALMGATVLALLCLLVLFGTADRAQAYPG
                     VGDFVRGQVVVKLKPTGATIENINTSYGSTTLERFPGSTGIYLLKLQAGSDLLGVVEK
                     MTADPRLLFAEPNFLSQAPEGDGRHKAWGVSASGPSPTDYAAKALGLSEAHAISRGSG
                     TTVAVLDTGAQLDHPALKANFEGVKRYDFVDSDTNPSDRQVGRDADGDGYKDEMVGHG
                     THVAGIVDLSAPGAKIMPLRVLDTEGYGDVFAIAKAVSYAERNAADVINLSLGTPSRS
                     RLLREAIDDAADKGVVTAAAAGNSNITVPHYPAAGTGVAAAVDGLLAVTSVDAYEKKS
                     HFANYGVWVDIAAPGEGIRSAFPVSVYASWSGTSMATPFIAGQAALVHAVDGSLGPAG
                     VERRIRCSARPLALKNPTYVGMLGAGHADVRASLDPGACR"
BASE COUNT          316 a          561 c          533 g          346 t
ORIGIN      
        1 ggcagccctg tattacatct cgatccgact cgaacgcggg acgcatgtct tcctcacgca
       61 tggtccaccg accccggagg ctcgcgctcc gtgctttcgt cggctcgcag ccaaggaccc
      121 aaccagaggg taccaagact ctcgttttgg tgagactcgt gcacatctct tctccccagc
      181 agggcgcact tctgacgaaa gcagatcata ctagccaggc tcgggcaggg tcattgctac
      241 agtctatact aagaacatga gcgtgatctg atgccctgca gtaccggacc accctactcg
      301 gtgaactagc ggcaagcacc cggatcgagg ctggcgcgta catcggcgtg gccggcgccc
      361 agcatgccga cgtaggtcgg gttcttcaag gcgaggggcc gggcgctaca ccgtatcctc
      421 ctttctacgc ccgcggggcc cagcgagccg tcgacggcgt ggacgagcgc cgcctggccg
      481 gcgatgaacg gcgttgccat agaggtcccg ctccaggagg cgtacacgct gaccgggaag
      541 gcgctgcgga taccctcgcc gggcgcggct atgtccaccc ataccccgta gttggcgaag
      601 tgcgactttt tctcgtatgc gtccaccgag gtgacggcca gcagcccatc cacggcggcc
      661 gcgacgccgg ttcccgccgc agggtaatgc ggcacagtga tattcgagtt gcccgcggcg
      721 gcggcggtga cgacgccctt gtcggcagcg tcgtcaatcg cttctcggag caaccgcgac
      781 cggctgggcg taccgagtga gaggttgatc acatcggcag cgttgcgttc tgcgtaggat
      841 actgccttgg cgatggcgaa aacgtcgccg tagccttccg tatccagaac ccggagcggc
      901 atgatctttg ccccgggcgc cgagaggtcc acgatccccg ctacgtgcgt accgtggccc
      961 accatctcgt ccttgtagcc gtcgccgtcc gcatcgcgac cgacctggcg gtccgaaggg
     1021 ttggtgtcgc tgtccacgaa gtcgtagcgt ttgacgccct cgaaattggc cttcagcgct
     1081 gggtggtcga gctgcgcccc ggtatccaag actgcgaccg tcgtgcccga gccccggctg
     1141 atagcatgtg cctcagaaag gccgagcgct ttcgccgcgt agtcggtggg agacggcccg
     1201 ctggcgctta cgccccacgc cttgtgtcga ccatctccct cgggcgcttg agagagaaag
     1261 ttgggctctg cgaacagtaa gcgggggtcc gccgtcatct tttcgaccac ccccaataag
     1321 tcagagccgg cctgaagctt gagcaagtaa attccggtgc tgcccgggaa cctttcgagg
     1381 gtggtcgagc cgtaggaggt gttgatgttt tcgatggtgg cgccggtcgg cttaagcttt
     1441 actacaacct ggccgcgcac gaagtctccc actccggggt aggcctgcgc gcgatccgcg
     1501 gtgccgaaca gcaccaatag gcacaacagc gcaaggacgg tcgctcccat cagagcatag
     1561 cgttgcgaca gtgcacaagg cattgacgaa ccttggtctt tcacggcgcc tccttagatt
     1621 gtttttacgc ctaaggagta ccagcacctt cccctcccta agcgctacca atctactggt
     1681 atcggcccgc aaagtctact atttttggca aagtggtgta ttggtgtatt ttttgtcctc
     1741 accggacgca taacac
//
LOCUS       DASRWZ010000574         6585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1280986, whole genome shotgun sequence.
ACCESSION   DASRWZ010000574 DASRWZ010000000
VERSION     DASRWZ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280986"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFH60_09035"
     CDS             <1..670
                     /locus_tag="VFH60_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinyl-CoA synthetase subunit alpha"
                     /protein_id="HET6313967.1"
                     /translation="KMAVGLAYGYHVESEPVLSAEGERLRPDVGPLKEWQRYVRGLFS
                     GGTFCYEAMWQMKGLLDEPVFSNTPVSPDLALADPHMSHRHTCIDLGSDEFTMGRPHP
                     MIDFSTRSQRIVQEAADPEVAVLLLDVVLGFGAHPDPAGALAPALRQARDIAAGEGRT
                     LPIVVHVCGTEGDPQGLEKQRQTLLEAGALIAPSNAAAALLAAGIAGRLKPQATSTKS
                     AYAD"
     gene            747..2186
                     /locus_tag="VFH60_09040"
     CDS             747..2186
                     /locus_tag="VFH60_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HET6313968.1"
                     /translation="MPDNSIADLLRTGPTVLNVGVRTFADTLQQVGARHLHVDWQPPA
                     QGDPQLGALLAAISDDDTPGSLGERVKAANEEALQRILAADPVWEDVRPAREVWPGMD
                     ERVLYHSGPPIEWDRMCGPMRGAVLGSILLEGWAGDEQAARDLAASGGVRFEPCHHHG
                     AVGPMAGLVSPSMPVFVVRNTDRGNYAYSSLNEGMGKVLRYGAYSPEVLARLRWMCDV
                     LGPTLQVGIRALGSVPLKPLMAQALQMGDELHNRHVAATSLLFKTLAPALIRALDDRE
                     RAASALDPIAANNYFFLNLAMAACKAMLDSARDIPYSTVVVTMARNGVDFGIRVAGTG
                     DRWFTGPAAVPDGVYLPGFTEADAAGDIGDSAITETAGLGGFAMGGAPAVVKVVGGTP
                     DDALDFTRSMHTITLAQNPNWTMPALGFAGTPTGIDVRKVLDTNTLPIINTGIAHKEA
                     GVGQVGAGLVRPPMQCFTEALRALGEAIT"
     gene            2190..3161
                     /locus_tag="VFH60_09045"
     CDS             2190..3161
                     /locus_tag="VFH60_09045"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HET6313969.1"
                     /translation="MMHHFTAISAGPELAKLAGARGRVLAVLSNAVYLRGEAGHITGI
                     VRSLAEDGPFTLRVTEIDLLVRLLKGREGNTFDSTSAFIELDGIARLELGRARQWQPQ
                     LPATIADVAERIRAVRALVGVLGGSWCDTGLCGLAAQLYGSHRSLPLPTEVPLLRTTP
                     TVSDTVSRKLAEKVTEFQAAASALEPEGASNALVGRLGLGMGLTPSGVDIAAGILASL
                     VWQARLGAVPPQFAQQMTDAVRRAAPARTNDISVRLLWHAGGGLLYAPAMELGEAMLG
                     GYVVSFAAQAQRLLSIGHSSGADMAIGLLAGIVAGIEIESRSKVPSC"
     gene            3211..5190
                     /locus_tag="VFH60_09050"
     CDS             3211..5190
                     /locus_tag="VFH60_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6313970.1"
                     /translation="MTQSYFAQDSNSPEIAWRPTPAYVERSRLRRFMLHHGIDTFGAL
                     VERYGADPAWFWQAVVEDLGLEWYEPYAQVLDLSGGIEWARWFAGGSYNYVRDAVDKH
                     AGGSLSAATAIGWEGEDGEVRNLTYDELSRQVNQAANALRALGVGRGDRVGVYMPMLP
                     ETAVAVLAISKIGAIYIPVFSGYGGAAIASRLQDCQAKLLITADGFYRAGKWVRMKEA
                     ADEAVAASPTVEHTLVVSRHGEARGSIAPWTQGRDVWWHELVGGQNEECDTVRTGADE
                     PYMIIYTSGTTGRPKGVVHLHAGFPVKAAQELAHCFDLHEGEALYWISDIGWMMGPWA
                     ITGTLMLGASCFLYEGSISHPQPDRLWSIVERHRITHLGISPTAVRSLMAHGDDAVRA
                     HDLSSLVVLAGAGEPWNPGPWRWLFDVVGGGKLPIINYSGGTEVSGGILSCNPLLPIK
                     PCAFSGPVPGMLPDVVDEDGTPVRGAVGELALRGPWVGKTEGFWGDNQRYLDTYWSRV
                     PGLWVHGDWARVDDDGFWYILGRSDDTIKVAGKRVGPAEVESAAASHQAVAEAAAIGV
                     PDTLKGEALVVLVVLRAGQEPGEELRGEIASVIVAHLGKSLKPQAVKFVAELPHTRNG
                     KTLRRLVRQGYLGEALGDLSSLENLSALEAVAAAF"
     gene            5417..5887
                     /locus_tag="VFH60_09055"
     CDS             5417..5887
                     /locus_tag="VFH60_09055"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HET6313971.1"
                     /translation="MELSGTHTIKAPQALVWKVLTDPETLRRTLPGCQRFEAQEDGSY
                     NITMSIGIAAIKGTYTGTVRLLNEQPPDSYSLKVAAKGGVGFIDGQGDFVLSPAGDNP
                     EHTRLSYTGQAQVGGKIAGVGQRLLHGGANLVIGQFFRALDKEVSQSEGAGKDL"
     gene            complement(5882..>6585)
                     /locus_tag="VFH60_09060"
     CDS             complement(5882..>6585)
                     /locus_tag="VFH60_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313972.1"
                     /translation="RVKTLSAVYERLLRSAQSAGMSPTVIGASIGGLSNLGKVLFDFD
                     PKLVAQKYAEGWVGVFEDIKRELQPRGRLRVEPGSRWPLFCRSIISGARFLSEFDTAE
                     DFYRWADTFYDNDSTRVSLPLMLSKRIDGFGFALACDFLKGLGYTNYGKPDTHLKAIF
                     TALGLSRSQDDFDVHAAIVRVAEHVGVTPYNVDMLFWLVGSGDFYLDNVKIGRQKEKF
                     IAYARPRLNCPPAPL"
BASE COUNT         1177 a         2107 c         2188 g         1113 t
ORIGIN      
        1 gaagatggcc gtgggcctcg cctatggcta ccacgtggag agcgagccgg tactgagcgc
       61 ggaaggagag cggttaagac cggacgtggg gccgctgaaa gagtggcagc ggtacgtgcg
      121 cggcctcttc tccggcggca ccttctgcta cgaggcgatg tggcagatga agggcctgct
      181 cgacgagccg gtattctcca atacgcccgt atcgcccgac ctggccctcg cggaccccca
      241 catgagccac cggcatacct gcatcgacct ggggtccgac gagttcacga tgggccgccc
      301 ccaccccatg atcgacttct ccacccgctc ccagcgcatc gtgcaagagg ccgccgaccc
      361 cgaagtagcg gtgctgctac tcgacgtggt gctcggcttc ggcgctcacc cggaccccgc
      421 cggtgccctc gccccggccc tccgccaggc cagggacata gccgccggcg aggggcgcac
      481 gctgcccata gtggtacatg tctgcggcac cgagggcgac ccgcaagggc ttgagaaaca
      541 gcgacagacg ctacttgagg cgggggcgct catcgcccca agcaacgccg ctgctgccct
      601 gcttgccgcc ggtatcgccg ggcggttgaa accgcaggca acatccacaa agtcggccta
      661 cgccgactaa gaaacagtcc ccgcaggggg acttcgtaat cgttgctcgc ggtttcaacc
      721 gccgggcatt tgttggagta ggccctatgc ccgacaatag catcgctgac cttctgcgca
      781 ccggccccac cgtgctaaac gtgggcgtca ggaccttcgc tgacacgttg cagcaggttg
      841 gcgccaggca cctgcacgtc gattggcagc ccccggcgca gggcgacccg cagttgggtg
      901 cgctacttgc cgctatatcg gacgacgata cgcccggcag cctgggagag cgcgtaaagg
      961 cggccaacga ggaggcactg caacgcatcc tggccgccga cccggtgtgg gaggacgtgc
     1021 gcccggcccg cgaggtctgg cccggcatgg acgagcgcgt gctgtaccac tccggcccgc
     1081 ctatcgagtg ggaccggatg tgcgggccta tgcggggggc cgtactcggg tccatcctgc
     1141 tggaaggctg ggcgggtgac gagcaggccg cgcgggacct tgcggcctcc ggcggcgtgc
     1201 ggttcgagcc ttgccaccac cacggcgcgg tggggccgat ggcgggcctc gtctcgccct
     1261 ccatgccggt gttcgtggtg cgcaacaccg accggggcaa ctacgcctat tcgtcgctca
     1321 acgagggcat gggcaaggtg ttgcgctatg gtgcctactc gccggaggtg ctggcccggc
     1381 tgcgctggat gtgcgacgtg ctggggccga ccctacaggt gggcatcagg gcgctcggca
     1441 gcgtgcccct aaagccgctc atggcccagg cattgcaaat gggcgacgag ctgcacaacc
     1501 gccacgtggc tgccacctcc ctgctcttca agacgctcgc ccccgccctc atacgcgccc
     1561 tggacgaccg cgaaagggcc gcctccgccc tcgacccaat cgccgccaac aattacttct
     1621 tcctcaacct cgccatggcc gcctgcaaag ccatgctcga tagcgcccgc gatataccct
     1681 attccaccgt ggtggtcact atggcccgca acggcgtcga tttcggcatc cgggtagcgg
     1741 gcacgggcga caggtggttc acgggacctg cggcggtgcc cgacggcgtc tacttgcccg
     1801 gcttcaccga ggccgacgcg gcaggcgaca taggcgacag cgcgataacc gagacggcgg
     1861 ggttgggcgg cttcgcgatg gggggcgcgc ccgcggtggt gaaggtggtc ggcggcactc
     1921 ccgacgacgc cctggacttc acgcgctcga tgcacactat cacgctcgcc cagaacccca
     1981 actggacgat gcccgccctc ggcttcgcgg gcacccccac gggcatagac gtgcgcaagg
     2041 tgctcgacac caacaccctg ccgattatca acacaggcat cgcccacaaa gaggcaggcg
     2101 tgggccaggt gggggccggt ctggtgcgcc cgcccatgca gtgcttcacc gaggccctca
     2161 gagctttggg tgaggccata acatagcgca tgatgcacca cttcacagcc atatcggcgg
     2221 gaccggagtt agcaaagctg gcaggcgctc gcgggagagt gctggcggtg ctgagcaacg
     2281 ctgtgtacct gcgcggtgag gcgggccata tcaccggcat cgtgaggtcg ctagcggagg
     2341 atggcccctt caccttgcgt gtcactgaaa tcgacttgct cgtccggttg ctgaaggggc
     2401 gggagggtaa cacgtttgac agcacgtccg ctttcatcga gttggatggc atagccaggc
     2461 ttgagttggg ccgtgcgagg cagtggcagc cacagctacc cgcaacaata gcagacgtgg
     2521 cagagcggat aagggctgtg agggcgcttg ttggcgtgct gggtggaagt tggtgcgaca
     2581 ctgggctatg tgggctcgcc gctcagctct acgggtcgca tcgcagcctg ccactaccca
     2641 ccgaagtgcc tctgctgagg actaccccta ccgtaagcga tacagtgtcc cgcaagctgg
     2701 cagagaaggt gacagagttc caggctgcgg catccgcgtt ggagccggaa ggcgcgtcca
     2761 acgccctggt tggccggctt gggttgggca tgggcctgac gccctccggt gtcgacattg
     2821 cggccggcat actcgcctcg ctggtgtggc aggcccgttt aggcgcggta ccgcctcagt
     2881 tcgcgcaaca gatgacagac gcggtacgcc gggccgcacc cgccaggacg aacgatatta
     2941 gcgtgcgcct actctggcac gcgggcggcg gactgctcta cgcacctgct atggagcttg
     3001 gcgaggccat gcttggggga tatgtagtgt ctttcgcggc gcaggcccag cgcctcctat
     3061 ccatcggcca cagttcgggg gctgacatgg cgatagggct gctggccggg atagtggccg
     3121 gtatcgagat cgagtcgagg agcaaagtcc caagctgctg acccgctgag cagtacgcta
     3181 caggcaccac gcgagtgcct ttcgaggcct atgacccaaa gctatttcgc ccaggacagc
     3241 aattcgcccg aaatcgcctg gcgtcccacg cccgcctacg tcgagcgcag ccgcctgcgc
     3301 cgcttcatgc tccatcacgg catcgacacg ttcggcgcgc tggtagagcg atatggggcc
     3361 gaccctgcct ggttctggca ggcagtggtg gaggaccttg gcctcgaatg gtacgagccg
     3421 tacgcgcagg tgctggacct ctccgggggc atagagtggg cgcgatggtt cgcgggcggc
     3481 agctataact acgtgcgcga tgccgtggac aagcatgcag gcggctccct ctctgccgcg
     3541 accgctatcg gctgggaggg ggaggacggc gaggtccgca atctgaccta cgatgagctt
     3601 tcccggcagg tcaaccaggc cgccaacgcc ctccgcgcgc tcggcgtagg caggggcgac
     3661 cgggtgggcg tgtacatgcc gatgctgccc gaaacggcgg tggcagtcct ggcaatctcc
     3721 aagataggcg cgatttacat tccggtcttc tccggctacg gtggcgctgc tatcgcctct
     3781 cgcctgcaag actgccaggc aaagctgcta atcacggcgg acggctttta ccgggcgggc
     3841 aagtgggtgc gcatgaaaga ggccgccgac gaagccgtcg ccgcttcccc cacggtggag
     3901 cacacgctcg tcgtgagcag gcacggcgag gcacggggca gcatcgcacc ctggacccaa
     3961 ggccgggacg tgtggtggca cgagttggtc ggcgggcaga atgaagagtg cgacacagtg
     4021 cgcacgggcg cggacgagcc gtacatgatt atttacacct cgggcacgac cggcaggccc
     4081 aaaggcgtgg tgcacctgca cgcgggcttc ccggtgaagg cggcgcagga actggcccat
     4141 tgcttcgacc tgcacgaggg cgaggcgctc tactggatca gcgacatcgg ctggatgatg
     4201 gggccgtggg cgattacagg cactctcatg ctgggggcct cctgcttcct gtacgaaggc
     4261 tccatcagcc acccgcagcc cgaccggctc tggtcgatag tcgagcggca ccggattacc
     4321 cacctcggca tctcgcccac cgccgtgcgc tccctcatgg cacacggcga cgacgcggtg
     4381 cgcgcccacg acctgtcgag cctggtggtg cttgccgggg cgggcgagcc gtggaacccc
     4441 ggcccctggc ggtggctgtt cgacgtggtg ggcggcggca agctccccat catcaactac
     4501 tccggcggca ccgaggtatc gggcggcatc ctgagctgca accccctgct gcccatcaag
     4561 ccgtgcgcgt tcagcggtcc cgtccccggt atgctgcccg acgtggtgga cgaggacggt
     4621 actcccgtgc gtggtgccgt cggggagttg gcgttgcgcg ggccgtgggt gggcaagacc
     4681 gagggcttct ggggcgacaa ccagcgttac ctcgacacct actggtccag ggtgcccggc
     4741 ctgtgggtgc acggcgactg ggcgcgggtg gacgacgacg gcttctggta catcctggga
     4801 cgctcggacg acacgatcaa ggtggcgggt aagcgcgtcg gccccgccga ggtggagtcg
     4861 gcggcagcgt cgcaccaggc ggtggccgaa gcagcggcta taggcgtgcc cgataccctc
     4921 aaaggtgagg cactggtggt gctcgtcgta ttgcgcgcgg gacaggagcc gggcgaggag
     4981 ctacgaggcg agatagcgtc cgtcatagtg gcccacctgg gcaaatcgct caagccccag
     5041 gccgtcaagt tcgtagcgga gctgccgcac acccgcaacg gcaagacgct gcgccgcctg
     5101 gtgcgccagg gctacctggg cgaggcgttg ggcgacctgt cgtcgctgga gaacctatcg
     5161 gcgttggagg cggtggcggc ggctttctag gcgtgatgca tggtcattct tggggaagga
     5221 agtaccgaat gacgccctgt gggggtgccc tgccaatgaa ttggcgggct taccagagac
     5281 gaagtctcct tcggagacta catgatgttg cttagtcccc tatgggactt tgcctcttgt
     5341 aagcccgtgg ctttagccac agggccacgg catgtggtag acttggtacc gcacagcctc
     5401 ataccaggag cttctcatgg aactatccgg cacgcacacc atcaaagccc cacaagccct
     5461 cgtctggaag gtactcaccg accctgagac gctgcggcgc accctccccg gctgccagag
     5521 gttcgaggca caggaggacg gcagctacaa tatcaccatg agcatcggca tcgcggcgat
     5581 aaagggcacc tacaccggca ccgtgcgcct gctcaacgaa cagccccccg actcctacag
     5641 cctgaaggtc gcggccaaag gcggggtcgg cttcatcgac ggccagggcg acttcgtgct
     5701 ctcccccgcg ggtgacaacc ccgaacacac caggctaagc tataccgggc aggcccaggt
     5761 cgggggcaag atagccgggg tcggccagcg cctgttacac ggaggggcta acctcgtcat
     5821 cggccagttc ttccgggcgc tggacaagga agtgtctcaa agcgaaggtg cgggcaaaga
     5881 cttatagagg agcaggcggg cagttgagcc tgggcctcgc atacgctata aacttctcct
     5941 tctgcctgcc aatcttgacg ttgtccaggt agaagtcacc actccctacg agccagaaaa
     6001 gcatatcgac gttgtacggg gtcacaccta cgtgctcggc aactcgaaca atggcagcat
     6061 ggacgtcgaa atcgtcttgt gagcggctca gaccgagagc cgtgaagatc gccttgaggt
     6121 gagtgtcagg ctttccatag ttggtgtagc caagtccctt caagaagtcg caggcaagcg
     6181 caaaaccgaa accatcaatc ctcttgctta acatcaatgg tagagacact cgggtgctat
     6241 cgttgtcgta gaaggtatcg gcccagcggt agaaatcctc ggcagtgtcg aactcggaga
     6301 ggaagcgcgc gcccgagatt atagacctgc agaatagcgg ccacctgctt cctggttcca
     6361 cgcgtaaccg gcccctgggt tgtaactctc tcttgatgtc ctcgaacacc cccacccagc
     6421 cttctgcgta cttttgggct accagcttcg ggtcgaagtc aaagagcacc ttgcccaggt
     6481 tgctgaggcc ccctattgaa gcgcctataa cggtagggct catgcctgcg ctctgtgcgg
     6541 accggagaag ccgctcatag acggcgctca aggtcttcac gcgtt
//
LOCUS       DASRWZ010000575         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_800949, whole genome shotgun sequence.
ACCESSION   DASRWZ010000575 DASRWZ010000000
VERSION     DASRWZ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800949"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1457)
                     /locus_tag="VFH60_09065"
     CDS             complement(177..1457)
                     /locus_tag="VFH60_09065"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET6313973.1"
                     /translation="MDKRAREIIGLPVVTMNRGTKIYDIEDMVIDPERGQVLALVVQE
                     AAWLHSARAIPFGRINVIGQDAVVIPDGRAVIEVNRDPVLKRLYNQQIIRGLRVLTDD
                     GRRLGTVADMTLDPKTGEIKGYFVSLGLGLTVGQGTRWLPAEQIISIGQRVMFVPAAV
                     AELFEQQIGGISGALDSAGGKLRAAGAKLNTQLEQVGTKVRDTVPARVGGTLVGRTAH
                     QIVRDKEGNALVLVGDTITQETVDAARASGRMPQLMMSAGTGPTQNSLSSLGDQAAQS
                     LRDIPNEARTLWNQLTGGYSRRVDMADEKAVDRRVKDALGRPVTRVILDGNDNIILNT
                     GDIVTNHAIQAARAAGVLDILIDSIYVARPKLALEDMKASRSGDASLDNVSGQPIESP
                     PVAPATAPEGAEVPQAGGQPAGSAAVGDNGPAGV"
     gene            complement(1551..1721)
                     /locus_tag="VFH60_09070"
     CDS             complement(1551..1721)
                     /locus_tag="VFH60_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313974.1"
                     /translation="MARFQGNTHAGSDRLAATLLVLVLLIVIGFLFWDFSDPNGFRIF
                     WDNLADAFNIRR"
     gene            complement(1980..>3717)
                     /locus_tag="VFH60_09075"
     CDS             complement(1980..>3717)
                     /locus_tag="VFH60_09075"
                     /inference="COORDINATES: protein motif:HMM:NF042625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6541 family protein"
                     /protein_id="HET6313975.1"
                     /translation="QVGGIGIDAYHHTMISQLFVEQGGIPANYQPYAPLVSFTYHYGF
                     HSLAASWAWLSGRTSPDEMMLVMPQMGQIADTIPVLTLTLFAWKMLGNRWAGLAAGAL
                     VGLYSIIPAFYVNWSRFTQGLGLALLPLAWVLLMEVLDRPLTRRQTAPAATSTPEPPP
                     PDDKQAPPKPQPAQRSGDVTWQTALRQSGPYMLAVIGAAGLALSHYRIVAIYAVFVAF
                     YVLWRMVRAVRQKAGAAEVLATARRVVTVAVLSVATLLPWIVNLQGNFGINEVSKGNA
                     PGREKYYDIAERLGPDILNHPSLYLLCALALGGIGWAVKRRDLLPLLPGITWVVVLLY
                     SNPYLFPLPEGMGLPGAGAIDLVTVLSGVYVPLCLMAGYTLARFASWVITLADGTPGA
                     RQRLWRVASGGLIGAVVLLGGLASGMQLAPVVVKDNKPYLTPGDVNVMLWMRDNLPQS
                     SYVLAEPFAFDWSPNNLLGTDAAIWVPLIAGLPSSVPPITAYNERPADPNYIKKMRDL
                     LRAEPLVRKVQDTGQLLPTTVDWEALKQAGITHIFAGSRSEYIDMTFLFDNPDSVTPL
                     YHHDNAWVFALR"
BASE COUNT          696 a         1155 c         1188 g          678 t
ORIGIN      
        1 tcgcgttctg caaagcgatg tacagcccgg tcacaacgaa ccccacgccg accaccaccc
       61 tcaggaactc atcgaatatc agcaccaaaa tgccgaggac cacgagcagg atgcccatga
      121 gaagtctggt attcaaagcc ttgtacctcc gaaaatgcta gtgcgaagcg ggacggctag
      181 acgcccgccg gtccgttatc tccgacagcc gcgctgcccg ccggttggcc cccggcctgc
      241 ggcacctctg ccccttcagg agcggtcgcg ggtgcgacag gcggcgactc tatgggctgc
      301 ccgctgacgt tgtccaggct ggcgtcgccg ctccttgagg ctttcatgtc ctccagggca
      361 agcttgggac gggccacgta gatcgagtct atcaggatgt cgagcacgcc cgccgcccgt
      421 gcggcctgta tcgcgtggtt agtcacgatg tcgcccgtgt tcaggatgat gttgtcgttg
      481 ccgtcgagga tgacgcgggt cacagggcgg cccagggcgt ccttgacgcg cctatccacc
      541 gccttctcgt cggccatgtc taccctgcga ctgtagccgc cggtaagctg gttccacagg
      601 gtgcgggcct cgttggggat gtcgcggagg ctctgcgccg cctggtcgcc cagcgagctc
      661 aggctgttct gggtggggcc tgtgcccgcc gacatcatga gctggggcat gcgcccggag
      721 gccctggcgg cgtccaccgt ctcctgcgtg atcgtgtccc cgaccagcac gagggcgtta
      781 ccctctttgt cgcgcacaat ctggtgagcg gtgcgcccca cgagcgtgcc acccacccgc
      841 gccggcacgg tgtcgcgcac cttcgtgccg acctgctcta gctgcgtgtt gagcttggcg
      901 cctgccgccc gcagcttccc gcccgcgctg tccagcgccc ctgagatgcc gccgatctgc
      961 tgctcgaaca gctccgcgac ggcggcgggc acgaacatga cgcgctgacc gatgctgatg
     1021 atttgctcgg ctgggagcca gcgggtgccc tgcccgacgg tcagtccgag gccgagggac
     1081 acgaaatagc ctttgatctc gcccgtttta gggtcgagcg tcatgtcggc caccgtgccc
     1141 aggcggcgac cgtcatcggt gagcacgcgg aggccgcgta tgatttgctg gttgtacagg
     1201 cgcttcagca cgggatcacg gttcacctcg atcacggcgc gcccgtcggg gatcacaacg
     1261 gcgtcctggc ctatcacgtt gatgcgaccg aacggtatgg cccttgccga gtggagccag
     1321 gcagcctcct gcaccacgag cgcgagcacc tgcccccgct ccgggtcgat caccatatcc
     1381 tcgatgtcgt aaatcttggt gccccggttc atcgtgacca ccggtaggcc gattatctct
     1441 ctagccctct tgtccatcag gcatactcct gaacttgaaa cctgaaacgc cggcgctatc
     1501 tacaaggccg gacgctcgca cgccggtact gcttaccagc cgagcatgag ctaacgtctg
     1561 atgttgaaag catcggctaa gttgtcccaa aagatgcgga agccgttcgg gtcggaaaag
     1621 tcccagaaga ggaagccgat tacgatgaga agtaccagca caagcaaagt ggcagcgagg
     1681 cggtccgacc ctgcatgagt gttgccctga aatcttgcca atgcacacct cccggcgtgt
     1741 agcaacttat gacgcaaccc agaaggcaag cgtcaagtat ctttcgccca cgctgttata
     1801 gctgcttgca aaaggtgtgc catgtgttgg gggaggagtt gttagttgtt agttgttagt
     1861 tgttagtgaa tgagcgcaaa gttgcaaaga gcgcagagga ggagccgaag ttcggatcgt
     1921 tcgtatgtga gtactcgaca ctctctacta acaactaaca actaacaact aacaacgcgt
     1981 cagcggagcg cgaacaccca ggcgttgtcg tggtggtaga ggggggttac ggagtcgggg
     2041 ttgtcgaata ggaaggtcat gtctatgtac tcgctgcggg agcctgcgaa gatgtgggtg
     2101 atgcccgctt gcttgagggc ctcccaatcg accgtggtgg gcagcagttg gcctgtatct
     2161 tgaacttttc gcacgagagg ctcggcacgg agtaggtccc gcatcttctt gatgtaattg
     2221 gggtcggcgg gcctctcgtt gtaggccgtt atgggaggta ccgaggaggg cagacccgcg
     2281 atgaggggca cccatatcgc ggcgtccgtg ccgagcaagt tgttgggaga ccaatcgaaa
     2341 gcaaacggct cggcaagcac gtatgagctt tggggtaagt tgtctctcat ccagagcatg
     2401 acgtttacgt cgccgggcgt gaggtagggc ttgttgtcct tcaccaccac gggtgccaac
     2461 tgcatgccgg aggcgaggcc gcccagcagc accaccgccc caatcagccc gccggacgcg
     2521 accctccaca accgctgcct ggcccccggc gtgccgtcgg ctagcgtgat gacccaggac
     2581 gcgaaacgcg ccagggtgta cccggccatg aggcagagag gcacgtatac gcctgatagc
     2641 acagtcacca ggtcgatggc ccctgcgccg ggcagtccca tgccctcggg cagcgggaaa
     2701 aggtagggat tggagtacag cagcaccacc acccaggtta taccgggcag caagggcaac
     2761 aggtcgcgtc gcttcactgc ccagccgata ccacccaggg cgagggcgca caacagatac
     2821 agagaggggt ggttcagaat gtcggggccg agacgctcgg cgatgtcgta gtatttctcg
     2881 cggccgggtg cgttcccctt gctcacctcg ttgatgccga agttaccctg caagttcacg
     2941 atccagggta gcagggttgc caccgacagc accgcgaccg tcacgacccg cctggccgtg
     3001 gctagcacct ccgccgcgcc cgctttctgc ctgaccgccc tcaccatgcg ccataacacg
     3061 tagaaggcca cgaacacagc atatatagca acaatccggt agtgagacag cgcgagtccc
     3121 gccgctccta tcaccgccag catgtacggc cccgactggc gtaaggccgt ctgccaggtg
     3181 acgtcgccgc tcctctgggc aggttgcggc tttggggggg cttgcttgtc atcgggtggc
     3241 ggaggctcag gcgtagaggt ggcggcgggg gcagtttggc gtcgggtcag tgggcggtcc
     3301 agcacctcca tgaggagcac ccaggcgagg ggcaagaggg ccaggccaag cccctgggtg
     3361 aacctgctcc aattgacgta gaaggccggg atgatggagt agaggcccac cagcgcgccc
     3421 gccgccagcc ccgcccagcg gttgcccagc atcttccacg cgaacagggt gagcgtcagc
     3481 acgggaatcg tgtcggctat ctgccccatc tgcggcatga ccagcatcat ctcgtcggga
     3541 gaagtgcggc ccgacagcca ggcccaggaa gcggccagcg agtggaaccc atagtggtag
     3601 gtgaacgaca ccaggggcgc gtagggctgg tagttggccg ggatgccgcc ctgctccacg
     3661 aaaagctgcg aaatcatggt gtggtggtag gcgtctatgc cgatgccgcc cacctgc
//
LOCUS       DASRWZ010000576         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_184433, whole genome shotgun sequence.
ACCESSION   DASRWZ010000576 DASRWZ010000000
VERSION     DASRWZ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184433"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1896)
                     /locus_tag="VFH60_09080"
     CDS             complement(136..1896)
                     /locus_tag="VFH60_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6313976.1"
                     /translation="MSETTFADLQLSEGMLATVAELGYEAPTPIQEQTIRLLIEGRDV
                     IAQAQTGTGKTAAFAIPIVECVDQESKLTQALVLAPTRELAVQVAEAVHRLGHSKKLS
                     VLPVYGGQPIERQLHALSRGVQVVIGTPGRVLDHLRRGTLQLSDIKYVVLDEADEMLD
                     MGFLEDIETILDSAPPGRQMALFSATIPPRIAALAQRYLQDPVQVMVQTAQMSVPTIE
                     QFYVEVTPRNKLDALTRVLDHEEPESAMIFARTRRDVDELGEALQSRGFDAETLHGDL
                     NQTQRDRVLQRFRSGQVDLLVATQVAARGLDITGVTHVFNYAIPEDAESYVHRIGRTG
                     RAGRAGKAITLVQPNEIRWLRVIERIIGQKITPMRLPTLVDIEARRREAMKACIRDKI
                     AAGNLLPFMQMVTELAEEFDLAEIAAAAAKMASDGERPLTEMVEPAQARAISDGVERG
                     MVRLVMNLGREAGVRPGDIVGAIANEAGIPGRAIGAIDIYDRVAFVEVPSDDRDRVVE
                     ALRNTTIKGRKLQVEVADGGSGKSAPPSAPRQFRTPRGGPDGARSDRDGRTGRRSEGK
                     AYPSRRDGGGATRPRKRRDG"
BASE COUNT          327 a          714 c          639 g          386 t
ORIGIN      
        1 ccagcgccct actgcgccta ccgacaatgt ttgcgccctg acattaaact gcgccgaacg
       61 cacccctaca ggccactccg ccggcatcca aaccccaaat gtgccacact gctacagcca
      121 ggcagcatgc gctccctatc cgtcgcggcg cttgcggggc cgtgtcgcac cgccaccgtc
      181 gcggcgcgaa gggtacgcct tgccttctga gcgcctgccg gtcctgccat ccctgtccga
      241 ccgggcaccg tccggccccc cacgaggggt gcggaactgc ctcggagcgg aagggggcgc
      301 agacttgccg cttccgccat ccgctacctc gacctgcaac ttgcggccct tgattgtcgt
      361 gttacgcaag gcctccacca cccggtctcg gtcgtcggaa ggcacttcca cgaaggccac
      421 ccggtcgtag atgtcgatag cgccgatagc gcgccccggt atgcccgctt cgttggctat
      481 cgcgcccacg atgtcgccag ggcgtacccc cgcctcgcgg cccaggttca tcaccagccg
      541 caccatgcct cgctccaccc catcgctgat ggcgcgggcc tgggccggtt ccaccatctc
      601 ggtgaggggg cgctcgccat cggaggccat cttcgcggcg gcggcggcta tctcggccag
      661 gtcgaactct tcggcaagct ccgtgaccat ctgcatgaag ggcagcaggt tgcccgccgc
      721 tatcttgtct cgaatacagg ccttcattgc ctctcgccgc cgggcctcta tatccaccaa
      781 cgtgggcagg cgcatcgggg tgatcttctg cccgataatg cgctctatta cccgcagcca
      841 ccttatctcg ttgggctgca cgagcgtaat agctttaccg gcccgacctg cgcggcccgt
      901 gcgcccgatg cggtgcacgt acgactcggc gtcctccggg atggcgtagt tgaacacgtg
      961 cgtcacaccc gtgatgtcga ggccgcgcgc cgctacctgc gtggcgacca gcagatcgac
     1021 ctgcccgctc cgaaagcgtt gcagcacacg gtcgcgttgg gtctggttca ggtcgccgtg
     1081 cagggtctcg gcatcgaacc cacgcgactg tagggcctcg ccaagctcgt ccacgtcgcg
     1141 cctggtgcgg gcgaatatca tggccgactc cggctcctcg tggtcgagca cgcgagtgag
     1201 ggcgtccagc ttgttgcgcg gggtgacctc cacgtagaac tgctctatcg tgggcaccga
     1261 catctgggcg gtctgcacca tcacctggac cgggtcctgc aggtagcgtt gggccagggc
     1321 ggctatgcgg ggcggtatgg tggccgaaaa gagggccatc tgccggcctg ggggcgcgct
     1381 gtcgaggatg gtctctatat cctccaggaa gcccatgtcg agcatttcgt ccgcctcgtc
     1441 cagcaccacg tacttgatgt cacttagctg gagggtgccg cgccgcaggt ggtccagcac
     1501 acggccgggc gtgccgatta caacctgcac cccgcgactg agggcgtgca actgcctctc
     1561 tataggttgc ccgccgtaca cgggcagcac gctcagcttc ttggagtgcc ccaggcggtg
     1621 caccgcctcc gccacctgga ccgcaagctc tcgcgtgggg gccagcacga gcgcctgggt
     1681 gagcttgctc tcctggtcca cgcactcaac aatgggtatc gcgaaagccg ccgtcttgcc
     1741 cgtgcccgtc tgcgcctggg caattacgtc ccggccctcg atgaggaggc gaatagtttg
     1801 ctcctggatg ggggttgggg cctcgtagcc aagctcggct accgtggcaa gcatgccttc
     1861 gctgagttgt aaatccgcaa acgtggtttc cgacatcagt tactgctcct agatatatcg
     1921 aggcacgggc aagtcccggc ccttaaagct attcatgcaa cctaaagcaa gttctgtgca
     1981 ccgaggtggc cggtccggcg tgcaatacgg cctgcggggg tgcgcacgtg agttgcggcc
     2041 tggccagtgc cggccagtgg cacaaa
//
LOCUS       DASRWZ010000577         4691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_321391, whole genome shotgun sequence.
ACCESSION   DASRWZ010000577 DASRWZ010000000
VERSION     DASRWZ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321391"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1233
                     /locus_tag="VFH60_09085"
     CDS             169..1233
                     /locus_tag="VFH60_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6313977.1"
                     /translation="MRVLMLAPLWYPISRDAQGGIETFLYSPIEALEREGCSVMLLAS
                     GDSRAAGELVPVVERNLYSLMHEGRAGEYVYYEQHQLRLASGLAHDFDVVHSHIGPGG
                     YVLSAHADVGGRVLHTVHSPVYEDICWFVGRHPDLLLSAVSEFQAERLRGAGARRCRV
                     VHNGIDTSGFPFNGRPGGGLLFLGRIEAAKGPDLAIDVARALGLTLTLAGPVIEREFF
                     KRSIEPRLDGQVRYVGVVDHETKCKLMGEAACMLLPFRQAEPFGMVSIEAMTCGTPVV
                     ALPNGALPEIVEPGVTGYLAAEVEALPGYVLQAMSLDRGRVRERATARFGIQHVAGKY
                     LDLYRDIAAGALEGERQPCA"
     gene            1224..2363
                     /locus_tag="VFH60_09090"
     CDS             1224..2363
                     /locus_tag="VFH60_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6313978.1"
                     /translation="MRIIVSGAIGRSYVGGQAWVYMQYLAGLRELGHDVYYLEDCGEE
                     SWVYHWEEQQLTTDLDYPAAYVNDCLALIGFGDRWIYRAGSEARGMPLEQLEQLCSGA
                     DLLLVRAVPLTVWRREYDLPRRRAFIDVDPGFTQISLLNGDPALTSTVDRCERLFTYG
                     QGIGRPDCLMPDAGRAWLKTVPPVALAYWPVAEEPDAAHFTTIIRWRGFRDAEYKGVK
                     YGQRDREFPHYIDLPRLTSQPFRVAALGADLESLAEHGWDAVSGEVESLTPLRYQRFI
                     QGSRAEFGVAKHLYVEMRTRWFSDRSVCYLASGRPVLVQSTGIEDLLPVGEGIVTFGS
                     LEAAVAGVDRINSDYERHRRAARHIAETCFAAERVLSDLLSGATN"
     gene            2373..3728
                     /locus_tag="VFH60_09095"
     CDS             2373..3728
                     /locus_tag="VFH60_09095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6313979.1"
                     /translation="MGNHKLSLNMRVAVFTDVFPALSETFITRQIVGLVELGHNVHIY
                     AQGRPGAGVPLQPEVAAHNLLAHTTYLAIPRASGYWEMPILPPWGQTWLPGAAKSIPN
                     LRRLLHALPHLARIFAIAPKVVLRTLDPREYGYAARSLSAVYRVSGLLGGRGAYDVAH
                     AHFGPVADKIRFVPSLWRVPLIASFHGYDVSAWPREKGAGVYERLFRAATLVTANSHY
                     TRQRVEALGCPSARIRVLRMGLDLGEFAFRERSAPEDGCVRILSVGRLVEKKGFEYSI
                     RALAQVRARHPQVQYGIVGDGPLRPHLEALATSLGLEGTVRFYGAAGREFVRRRLDEA
                     HIFVAPSVTAANGDVEGQGLVLQEAQACGIPVLATEHNGFPEGMLPGQSGFLVPERSA
                     EAIAERLEYMLERAGRWAEWGRAGRRHVEEHYDIRLLSRQLEGLYAEARHIYTSGKGR
                     R"
     gene            3725..>4691
                     /locus_tag="VFH60_09100"
     CDS             3725..>4691
                     /locus_tag="VFH60_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313980.1"
                     /translation="MRLFYYQDPGGNFGDDLNAWLWPRLIPELLDGDDSTLFVGIGSI
                     LDRRIPEGPCKIVFGTGVGYGLLPVLTEEWQVCCVRGPLSARALGLPPELAVTDSAAL
                     VRTVRSAPVERRHSVSFIPHFRTPARVQEAGIDLKRVCASAGINYIDPCGPVEEVLAG
                     IESSGKVIAEAMHGAIVADALRVPWLPVRLSERIRGLKWRDWCSSLRMEYKPLPFSPP
                     PGTAPGEALARFLDAANTDADPVLSSTGVLDEAVERLVAGLELLKQGRAGECGVQGSA
                     SFASDPGVLREVPWLYEMQAALKEVEGLVSPGSTFILVDEERWGGG"
BASE COUNT          771 a         1453 c         1646 g          821 t
ORIGIN      
        1 gttccaggag gcccaggacg gcctggagaa cgtgaccgcg aactactccc gccacgccac
       61 tgccgcgcgt gaactcgctg tagcttactt cgcaccggaa gtggtgctgc ctccgctgct
      121 tgagcgcgcc acgcagaaca agagccatgc tcggaaggtg caggttgtat gagagtgctc
      181 atgctggccc cgctatggta ccccatctcc agagacgcgc aggggggcat tgagactttc
      241 ctgtactctc ccatagaggc cctggagagg gagggttgct cggtgatgtt gctcgccagc
      301 ggcgactctc gtgcggccgg ggaactggtg cccgtggtgg agcgcaacct ctacagcctg
      361 atgcacgaag gccgcgcggg cgaatacgtc tactacgagc aacaccagtt gcgcctcgcg
      421 tcggggctgg cccatgactt cgacgtggtg cactcccaca tcggccccgg cggctacgtg
      481 ctttcggcgc atgcggacgt gggtgggcgg gtgctgcaca cagtccatag ccccgtgtac
      541 gaggatattt gctggtttgt ggggcggcac cctgacctac tgctgtcggc ggtgagcgaa
      601 ttccaggcag agcggctgcg gggggcgggt gccaggcgct gccgggtggt gcacaatggc
      661 atcgacacct cgggcttccc ctttaacggt cgccctgggg gcggcctgct cttcctgggc
      721 cgcatcgagg cggcgaaagg ccccgatctt gcgattgacg tggcgcgtgc cctcggtctc
      781 acgctcacgc tggccgggcc tgtgatcgag agggagttct tcaagcgcag catcgagcca
      841 cgcctggacg ggcaggtccg gtatgtgggg gtggtcgatc acgagacgaa atgcaagctg
      901 atgggcgagg ccgcctgcat gctcttgccc ttccgccagg ccgagccttt cggtatggtg
      961 agcatagaag ccatgacgtg cggcacgccg gtagtggcgc tgcccaacgg cgcattgcct
     1021 gagatagttg agcctggggt caccggctac cttgccgccg aggtggaggc tttgcccggc
     1081 tacgtattgc aggccatgtc gctcgaccgg ggcagggtcc gagagcgagc gacggccagg
     1141 tttggcatac agcacgtcgc cggtaagtac cttgacctgt acagggatat agccgcggga
     1201 gcgctggaag gggagcgcca gccatgcgca taatcgtgtc cggcgcgata gggcgctcat
     1261 acgtgggcgg gcaagcctgg gtgtacatgc agtacctggc cgggctgcgc gagctgggtc
     1321 acgacgttta ctacctggaa gattgcgggg aggagtcgtg ggtctaccac tgggaggagc
     1381 agcaactcac caccgacctc gattacccgg ccgcctacgt aaatgactgc ctggcgctga
     1441 taggcttcgg tgacaggtgg atttatcgcg cgggcagcga ggcgcgcggc atgcctctgg
     1501 agcaacttga gcagctatgc agcggggccg acctcctgct ggtgcgggcc gtgccgctga
     1561 ccgtctggcg cagggagtac gacctgcccc gccgccgggc gttcatagac gtggaccccg
     1621 gtttcacgca gatcagcctg ctgaatggcg accccgcgct cacctccaca gtggaccggt
     1681 gcgagcgact cttcacctac gggcagggca tcggcaggcc cgattgcctg atgcctgacg
     1741 cgggcagagc atggctcaag acggtgccgc ccgtggccct ggcctactgg cccgtcgctg
     1801 aagagccgga cgccgctcac tttaccacga tcatccggtg gcgcggcttc agggacgccg
     1861 agtacaaggg agtgaagtac gggcagcgcg accgcgagtt cccccactac atcgacctac
     1921 cgcgcctcac cagtcagccc tttcgcgtgg cagccctcgg ggccgacctg gagagcctgg
     1981 ccgagcatgg ctgggacgcc gtgtcgggcg aagtggagtc actgacgccc ctgcggtacc
     2041 agcgcttcat acaaggctca cgagcggaat tcggtgtggc gaagcacctc tacgtggaga
     2101 tgcgcacgcg ctggttcagc gaccgtagcg tgtgctacct ggcgtcgggc aggccggtgc
     2161 tcgtgcagag cacggggatc gaggacctgc tgcccgtagg cgagggcata gtcaccttcg
     2221 gctcgctaga ggcggccgtg gccggggtgg accgtatcaa ctccgattac gagcggcaca
     2281 ggcgcgcggc caggcacata gccgagacct gcttcgccgc cgagcgcgtg ctgtctgacc
     2341 tgctgtcggg tgctacaaac tgagacgtga ttatggggaa ccataagctg agcctcaata
     2401 tgcgcgtcgc ggtctttaca gacgtcttcc ccgcactttc agagacgttc atcaccaggc
     2461 agatagtggg cttggtggaa ctggggcaca acgtgcacat ttacgcccag ggacggcctg
     2521 gcgcgggagt gccgttgcag cccgaggttg ccgcgcacaa cctgctggcg cacactacat
     2581 acttagccat tccgcgtgcg tccggctact gggaaatgcc cattcttcct ccctggggcc
     2641 agacctggct gccgggagcc gcgaagtcca tacccaacct caggcgactc ctgcacgcgc
     2701 tgccccacct cgctcggatc ttcgcaatcg cgcccaaagt cgtgttgcgc acgctggacc
     2761 cacgggagta cggctacgct gccaggagcc tgtcggcggt gtaccgcgtc tctggtctgc
     2821 ttggtggtcg cggcgcatac gacgtggcgc acgcccattt cggcccggtg gctgacaaaa
     2881 tacgcttcgt cccctcactc tggcgggtgc cgctaatcgc cagcttccac ggctatgacg
     2941 tgtccgcgtg gccccgtgag aaaggggccg gtgtgtacga gcggctgttc cgtgcggcca
     3001 ctctcgtgac agccaacagc cactacacgc ggcagcgagt ggaggcgctt ggctgtccct
     3061 cggccaggat tcgcgtgctc aggatgggcc tggacctggg ggagttcgct ttccgcgagc
     3121 gctcggcacc ggaggatggc tgcgtgcgca tcttgagcgt gggcaggctg gtggagaaga
     3181 aaggcttcga gtatagcatc agggcgctgg cgcaggtgcg cgccaggcac ccgcaggtgc
     3241 agtacggcat agtgggcgat gggcctctcc gcccgcacct ggaagcgctg gcaacctcgc
     3301 tcgggttgga gggtaccgtg aggttctatg gcgcggcggg gcgcgagttt gtgcggcgca
     3361 ggctcgatga ggcgcacatc ttcgtcgccc ctagcgtgac cgcggcgaac ggcgacgtgg
     3421 aagggcaggg cctggtgcta caggaggccc aggcctgcgg cattcctgtg ctggccaccg
     3481 aacataacgg cttcccggag ggcatgctgc ccggccaatc gggcttcctg gtgcctgagc
     3541 gtagtgctga ggccatagcc gagcggctgg agtatatgct cgagcgcgcc ggccgctggg
     3601 ccgagtgggg ccgggccggg cgcaggcacg tggaggagca ctacgacatc cgcctgttga
     3661 gccgccaact ggagggtctg tacgcggagg ccaggcacat ctacacgtcc gggaagggcc
     3721 gcaggtgagg ctcttctact accaggaccc cggtggcaac ttcggggacg acctcaacgc
     3781 ctggctgtgg cctcgcctca tcccggagtt gctggatggc gacgacagta ccctgttcgt
     3841 cggtataggc agcatcctgg accggcgtat accggaaggc ccgtgcaaga tcgtgttcgg
     3901 cacgggcgtg gggtacgggc ttctgccggt gctgaccgag gaatggcagg tgtgctgcgt
     3961 gcgcggcccg ctctcggcca gggcactcgg cctgccgccc gagctggccg ttaccgactc
     4021 ggccgccctg gtgcgcacag tgagaagcgc gcccgtcgag aggcggcaca gcgtctcctt
     4081 catccctcac tttcgcacgc ccgcacgcgt ccaggaggcg ggtattgacc tcaaacgggt
     4141 ctgcgcgagc gcgggcataa actacatcga cccctgcggc cccgtggagg aggtgctcgc
     4201 gggcatagaa agctcgggca aagttatagc cgaggcgatg catggggcca tcgtggcgga
     4261 tgccctgagg gtaccgtggc tgccggtgcg gctgtcagaa cggatacggg gccttaagtg
     4321 gcgggactgg tgcagctcgc tgcgcatgga atataagcct cttccctttt caccaccgcc
     4381 ggggacggca ccaggcgaag cgctggcccg gttcctggac gcagcgaata ccgatgccga
     4441 tccggtcctc agctcaacgg gcgtgctcga tgaggccgtg gagcgactcg tcgccgggct
     4501 tgagctactg aaacaggggc gggccggtga gtgcggggtg caaggtagcg ccagcttcgc
     4561 ttccgacccg ggggtcctgc gggaggtgcc ctggctttac gagatgcaag ccgcactcaa
     4621 ggaggtggag ggtctcgtct cccctggatc aacgttcatc ctggtggacg aagagcgctg
     4681 gggcggcggg c
//
LOCUS       DASRWZ010000578         4814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_116019, whole genome shotgun sequence.
ACCESSION   DASRWZ010000578 DASRWZ010000000
VERSION     DASRWZ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116019"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..4532)
                     /locus_tag="VFH60_09105"
     CDS             complement(87..4532)
                     /locus_tag="VFH60_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6313981.1"
                     /translation="MQEIAHTDTRPNGHSPSMGVPVRVLLIANCPDDLDLIGGSLDKA
                     RRLRPELVGANGLAEGVALLASSKFDAVLLDLALLQEAGVDALRAVQACAPEVPFIAL
                     AGDHDGSLEALAAGTQDYLVKDEISAGLLERSLLYALERARSEALLSKKAEVEMRLSE
                     ERFRTLFEQSPLPTAILARDGRALQANQAFAELLSVSPERLASYNVYEDAQLQQAGHV
                     PHIRKVFEGETTDLPLFEYEGPGGKRWLRNFAYPIKDETGEVREVVFMGEDITEQKRA
                     EQRLRENEEQYRRVFEVTTDGLVINDVNGGVVEVNAAFCQMHGYTREELIRMSPAEFI
                     HPGSHNLLREFFETVSRGEKFQCQAQDVRKDGTVFDVEVHGATFMYKGEPHVLGVVRD
                     ITERVDSERLLKLKEEQYRAVFETTTDGLAIADLNMGTIVEVNPAFARMHGYTREEMA
                     GMLPTDFVHPADYEVQAEFVEKARVGERAYAQGQDVRKDGSVFSIEVHGVPFSHNNQP
                     HVLAIMRDITERLQAEKLLRDSEEQYRGIFEATTDGLVISTIEPDGNAGVLVDVNPAF
                     CEMHGFTREELIGQHPSIFVNPDNLEELADCFKIVQAGGEFHSVGLDRRKDGTTFDVE
                     VNASRFTYKGQPLVLGVIRNITEQKLAEQRLRESEEQYRGVFEATTDGLVITSIQEGD
                     SAAYIVDANPAFCEMHGRTREELIGQHPTVFIHPSGYDGMIEAFEAIKAGKPFAGEGL
                     DVRKDGSAFDVEVHAIRFMHKGESRVLAVIRNVTEQKAAEGRLRESEEQYRGVFESTL
                     DGLVVTALEGEMSVVDANPAFCQMHGFTREELIGKSPAIFVHPNSRAKLAEAMETVLN
                     GGKYLAQAMDVRKDGSEFHVEVHLTRFTRGDKVHSLGVVRDITERVQAYELLEQRVEE
                     RTRELSTLLEVSSNVNSTLELKPLLGRVLDQLKSVVDYAGAGILLVDGDELVMLDSRD
                     ADGPEPEMIGRRYQISRIPLVWKAISEQGWLAIPDVRAEDGIAREYQTAVGAEVLNTS
                     LSYINSYLGVRLMLKDKVIGHLTLSHWQPNYYTRDHARLATGLANQAAIAIENARLYE
                     QAQETTRRTATLARIASRVALGGPLRPTLDDLSTSVVGATGALACAIGVFDDKSGLLK
                     LHGTCNLPDGYAEGLDRILASGTKLLAQAAFEGRKPMVLHNMRRSVLDIPAYEPIHKF
                     MHAVPWDTVVAVPMIYQDKPMGVLLKYHLPGREFGDEQMGFFVAIADQIAVAVANGRL
                     FEQVQGKAALEERQRLARELHDSVTQELFSISLTSRSIQLLLEREGVTLPSITEKMAD
                     LRQLTQGALAEMRALIFELRPGALEEEGLLQALRKHAAAVQGREMLPVEVIADEASIP
                     RLKPNAEESLYRIGQEALHNVVKHAHAKRVQIKMDVEDGVLALQICDDGDGFDASQVP
                     AGHMGLNTMGQRVAALGGEYHVSSKPGEGTTVSVRLPVASQRLQS"
BASE COUNT          792 a         1653 c         1381 g          988 t
ORIGIN      
        1 ggtccttttg tactcgctaa accatcctta aactttgcgc tctttgcgcc tttgcggttt
       61 ctcctcttct caatagcagc acgctgctag ctttgcaggc gctggctggc gacgggtagg
      121 cgcaccgaca cagtcgtccc ctcgcccggt ttgctgctca catggtactc cccaccgagc
      181 gcggccaccc tctgccccat cgtgttcagg cccatatgcc ctgccggtac ctggctcgcg
      241 tcgaagccgt cgccgtcatc gcatatctgc aacgcgagga cgccatcctc cacgtccatc
      301 ttgatttgca cccgcttagc gtgggcgtgt ttcaccacgt tgtgcaacgc ctcctgccct
      361 atcctgtaga gcgactcctc ggcgttcggc ttcaggcgcg gtatgctcgc ctcgtccgcg
      421 atcacctcca ccggcagcat ctcgcgcccc tgcacggcgg cggcatgctt gcggagggcc
      481 tgcaacaagc cttcctcctc gagcgcgccg ggacgtagct cgaagatgag ggcgcgcatc
      541 tccgcgagtg cgccctgcgt aagctgccgc aggtcggcca tcttctcggt gatggacggc
      601 aacgtcaccc cctcgcgctc cagcagcagt tgtatgctgc gcgaggtgag actgatgctg
      661 aatagctcct gcgtcaccga gtcgtgcagc tccctcgcca gccgctgccg ctcctccaac
      721 gccgctttgc cctgcacctg ctcgaacagc cgcccgttcg ccactgccac cgctatctgg
      781 tcggctatcg ccacgaagaa gcccatctgc tcgtcgccga actcgcgccc cggcaggtgg
      841 tacttgagca gcacgcccat cggcttgtcc tggtagatca tcggcaccgc gacgacggta
      901 tcccacggca ccgcgtgcat gaatttgtgg ataggctcgt acgcaggtat gtcgagcact
      961 gaccggcgca tattgtgcag caccatcggc ttgcggccct cgaaggcggc ctgcgccagc
     1021 agcttcgtgc ccgacgccag tatccggtcc aaaccctcag cgtaaccgtc cggcaggttg
     1081 caggtgccat gcagcttcag caggccgctc ttgtcgtcga acacccctat tgcgcaggcc
     1141 agcgccccgg tcgcccccac aacggacgtg ctcaggtcgt ccagcgtggg ccgcagcgga
     1201 ccgcccagcg ccaccctgga ggcaatccgc gccagggtgg ccgtgcgccg cgtcgtctcc
     1261 tgggcctgct cgtagagcct ggcgttctct atggctatgg cagcctggtt tgccaggccg
     1321 gtagccagcc gggcgtggtc gcgtgtgtag tagttcggct gccagtggga gagggtcaga
     1381 tggccgataa ccttgtcctt cagcatgagc cgcacgccga ggtacgagtt tatgtagctg
     1441 aggctggtat tgagcacctc cgcgcccacc gctgtctggt actcccgcgc tatgccgtcc
     1501 tccgctcgaa cgtcggggat ggccagccac ccttgctccg atatggcctt ccagacgagg
     1561 ggtatccggc ttatctggta gcgccgcccg atcatctccg gttccgggcc gtcggcgtcg
     1621 cgagagtcga gcattactag ttcgtcgccg tccacgagca ggatgcccgc cccggcgtaa
     1681 tcgaccacgc ttttcaactg gtcgagcacc cggcccaaca gcggcttcag ctccagggtc
     1741 gagtttacgt tgctcgagac ctccagcaaa gtggatagct cgcgggtgcg ctcctctacg
     1801 cgttgctcca gcagttcgta agcctgcacc cgctccgtga tgtcgcgcac cacgcccagc
     1861 gagtggacct tgtcgccgcg cgtgaaccgg gtcaggtgca cctcgacgtg gaactcgctg
     1921 ccatctttgc gcacgtccat agcttgcgca aggtacttcc cgccgttcag caccgtctcc
     1981 atcgcttcgg ccagcttggc acggctgttg gggtgcacga atatagcagg cgacttgccg
     2041 attagctcct cgcgggtgaa gccgtgcatc tggcagaagg cggggttggc gtccaccacg
     2101 gacatctccc cctccagggc ggttaccaca aggccgtcga gggtagactc gaacacgcca
     2161 cggtactgct cctcgctctc ccgcaggcgt ccctccgccg ctttttgctc ggtgacgttt
     2221 ctgatcaccg ccaggacgcg cgactcgcct ttgtgcatga acctgatagc atgcacttcg
     2281 acgtcgaatg cgctgccgtc cttacgtacg tccaacccct cgcccgcgaa gggcttcccg
     2341 gccttgatcg cctcgaaagc ctctatcatc ccatcgtagc cgcttgggtg gataaagacc
     2401 gtggggtgct gcccgattag ctcctcgcga gtgcgcccgt gcatctcgca gaaggcggga
     2461 tttgcatcca ctatataggc agcactgtca ccttcctgta tgctggtaat cacgagacca
     2521 tcggtggtag cctcgaatac gccgcgatac tgctcctcgc tctcgcgcag gcgctgttcg
     2581 gccagcttct gctccgtaat gttgcgtatc acgcccagca cgaggggctg gcccttgtac
     2641 gtgaaccggc tggcatttac ctccacgtca aatgtggtgc cgtccttgcg cctgtccagg
     2701 ccgacggagt ggaactcgcc cccggcctgt actatcttga agcagtcggc tagctcctcc
     2761 aagttgtcgg ggttcacaaa aatgctgggg tgctggccta tcaattcttc gcgggtgaag
     2821 ccgtgcatct cacaaaacgc ggggttcacg tccaccagca cgcccgcgtt accatccggc
     2881 tctatagttg aaatcaccag gccgtcggtg gtcgcctcga atatgccccg gtactgctcc
     2941 tcgctgtccc ggagcagctt ttccgcctgc aaccgctccg tgatgtcgcg cattatcgcc
     3001 aggacgtggg gctggttgtt gtggctgaaa gggaccccgt gcacttcgat ggagaagacg
     3061 ctgccatcct tgcgaacgtc ctgcccctgg gcgtaggcgc gctccccgac cctcgccttc
     3121 tccacgaact ctgcctggac ctcatagtcg gccgggtgga cgaagtcggt gggaagcatc
     3181 ccggccatct cttcgcgggt gtacccgtgc atcctggcga aggcggggtt gacctcgact
     3241 atggtgccca tgttgagatc ggcgattgcc agcccatcgg tggtcgtctc gaatacggcg
     3301 cggtactgct cttccttaag cttgagcagc ctctcagagt ccacgcgctc agtgatgtcc
     3361 cgcaccacac cgagtacatg gggttcgcct ttgtacatga aggtcgcccc gtgtacctcc
     3421 acgtcgaata ctgtaccgtc cttgcgcacg tcctgggcct gacactggaa cttctcgcca
     3481 cggctcaccg tctcgaagaa ctcgcgcagc aggttgtggc tgccagggtg gatgaactcc
     3541 gccggggaca tcctgatcag ctcttcgcgg gtgtagccgt gcatctggca gaaggcggcg
     3601 ttcacctcca ccacgccccc gtttacgtcg ttgatgacca gcccgtccgt ggtgacctcg
     3661 aacacgcgcc ggtactgctc ttcgttctcc cgcaggcgct gctcggcccg cttctgctcg
     3721 gtgatgtcct cgcccatgaa caccacttcg cgcacctcgc ccgtttcgtc tttgataggg
     3781 taggcgaagt tgcgcagcca gcgcttgccg cccggacctt cgtactcgaa caggggtagg
     3841 tcggtcgttt caccctcgaa taccttgcgg atgtgcggca catgccccgc ctgctgtagt
     3901 tgcgcgtctt cgtagacgtt gtaactcgcc aatcgctccg gcgacaccga gagcagctcc
     3961 gcgaaagcct ggttcgcctg caacgccctg ccgtcgcggg cgaggatagc ggtgggcagg
     4021 ggtgactgct cgaacaacgt gcggaaccgc tcttccgaca ggcgcatctc cacctcggcc
     4081 ttcttgctca gcaacgcttc ggaccgcgcg cgttccagcg catacagcag cgaccgctcc
     4141 aacaagcccg cgctgatctc atccttcacg aggtagtcct gcgtgccggc cgccagcgcc
     4201 tccaggctgc cgtcatggtc gcccgcgagc gcgataaagg gcacttcggg ggcgcaagcc
     4261 tgcacagccc gcagcgcgtc aacgcccgcc tcctgtagca gggccaggtc gagcagcaca
     4321 gcatcgaact tgctagaagc cagcagcgcc acgccctcgg cgagaccgtt cgcaccaacc
     4381 agctctggcc gcagccgcct tgccttgtcc agactgccgc caatcaagtc caggtcgtca
     4441 gggcagttag cgataagcaa aacacgaaca ggcacgccca tagaggggct gtgcccgttc
     4501 ggacgtgtgt ccgtatgcgc gatttcttgc attctcttcc ctcggtctag gtgcaaaaaa
     4561 gggctgcacg tccataacgt gcagggtgcg agcatcgccc gtacggtaca gcttcacggc
     4621 agaagcgctt acgagcacct aaagcttatc aaaactctac ggcttctgca actactatat
     4681 ataaacaata tactcgccta tacttgctct aacttgctgg ttgccaccct gcgtaacgcc
     4741 gccagcagag cctctgccgg ttcgtgcttg gcgacgaacg cggagacgcc catagcctcg
     4801 gcgcgcgccc ggtt
//
LOCUS       DASRWZ010000579         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_870320, whole genome shotgun sequence.
ACCESSION   DASRWZ010000579 DASRWZ010000000
VERSION     DASRWZ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870320"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFH60_09110"
     CDS             <1..653
                     /locus_tag="VFH60_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HET6313982.1"
                     /translation="ALLFQDGGLLALGGNIFNMAVVGVAVSYAVYSLVHRAARGRRAS
                     ILVGGFVTAWLSIFVTALGVGLQLAASGTARADLAIPAMGGIHALIGIGEGLITAGAL
                     AFVSATRPDLLKLGEARPAGGRAIVAGGLALALALVVLSPIASANPDGLEWVAEQQNF
                     LGQAQSPQFNIFQDYAFPGISSPALATIMAGVLGTLIVFGVALAVAHSRRRRQPTA"
     gene            662..1465
                     /gene="cbiQ"
                     /locus_tag="VFH60_09115"
     CDS             662..1465
                     /gene="cbiQ"
                     /locus_tag="VFH60_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HET6313983.1"
                     /translation="MHVHFLDPYRAGTSPVHTLDPRIKLVLALAFILTTSLTPIGAWP
                     IYVLLLAIALSVVILSELGVGKVLRRSLLALPFVLAALPLAFSMGAQRLFAIPLGFGE
                     LTVFAEGLERFASIAMRSWISVQVAIVLASTTPFPDLMLAMRAIRVPRLLVSIFGLMW
                     RYIFVMADEAQRLMRARESRSAVAAMPGLKAGGSVGWRARVTGGMAGNLFVRSLERGD
                     RIYSAMSARGYDGEVRTLGLPRVSPGEWALLFGGVILLLGLLWLSFLLS"
     gene            1474..2211
                     /locus_tag="VFH60_09120"
     CDS             1474..2211
                     /locus_tag="VFH60_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6313984.1"
                     /translation="MILRPTDLETPHALHIERLSFSYPDGHPALHDVTLDVRQGEKVA
                     IVGPNGAGKSTLILHLNGILTGQGTVRVCGLDVVKPNLGRVRGLVGMVFQLPDDQLFS
                     PTVFDDVAFGPLYQGLPEAEVRLRVEQALAAVGMSEYVHRVSHHLSVGEKKRIAVATV
                     LSMQPEMLVLDEPSAGLDPRARRGLINLLRDLPQTILVTTHDLPLVRDLCPRTIIMDG
                     GTIVADGRTADILADQALLTAHGLEMP"
BASE COUNT          330 a          729 c          786 g          454 t
ORIGIN      
        1 aggcgctgct cttccaggat ggtgggctgc tggcgttggg cggcaatatc ttcaacatgg
       61 ccgtcgtagg cgtagccgtt tcttatgccg tttacagcct cgtgcacagg gcagccaggg
      121 gcaggcgagc gagtatcctg gtgggcggct ttgtcacggc ttggctttcg atctttgtta
      181 ctgccctggg cgtggggctt cagcttgccg cctcgggcac cgcacgcgcc gacctggcta
      241 tcccggcgat gggcggcatc cacgcgctca taggcatcgg tgagggcctt atcaccgctg
      301 gcgcgctggc cttcgtgtcg gccacccggc ccgacctgct caagctgggg gaggcgcgtc
      361 ccgcgggcgg cagggccatt gtggcgggcg ggctggcgct tgcgctggcg ctggtggtgc
      421 tctcgcccat agcctccgcg aaccccgacg ggctggagtg ggtggccgag cagcaaaact
      481 tcctgggtca ggcgcaaagc ccccagttca acatcttcca ggactacgcc ttccccggca
      541 tcagcagccc cgcgctggcg accataatgg cgggcgtgct gggcaccctg atcgtgttcg
      601 gcgtggcgct ggcggtggcc cacagccgca gacgacgcca gcccacggcg taaaagcccc
      661 cgtgcacgtt cacttcctcg acccttacag ggcaggcacc agcccggtcc acaccctgga
      721 cccccgtatc aaattggtgc ttgccctggc cttcatcctg actaccagcc tgacccctat
      781 cggagcctgg cctatttacg tactcctgct ggctatcgcg ctctcggtgg tgattctgtc
      841 ggagctaggg gtgggcaagg tgctcaggcg gtcgttgctg gcgctgccgt tcgtgcttgc
      901 ggccctgccg ctggccttct cgatgggcgc gcaacgtctg ttcgccatcc cgttgggctt
      961 cggggaattg acggtgttcg ccgagggtct ggaacggttc gccagcatag cgatgcgctc
     1021 ctggatatcc gtacaggtgg cgattgtgct ggcctccacc accccgttcc ccgacctcat
     1081 gctggctatg cgcgccatac gggtgcccag gctgctggtg agcatattcg ggctgatgtg
     1141 gcgttacatc ttcgtcatgg cggacgaagc gcagcggctc atgagggcca gagagtcgcg
     1201 gagtgcagtg gcagctatgc cggggctgaa ggcaggcggg agcgtcggct ggcgggcgcg
     1261 ggtcacgggc ggtatggcgg gcaacctgtt cgtgcgctcg ttagagcggg gagatcgtat
     1321 ctactccgcg atgtccgccc gcggctacga cggcgaggtg cggacgcttg ggctgccccg
     1381 cgtgtcgccg ggtgagtggg cgctgctctt tgggggcgtc attctgctgc tggggctgtt
     1441 atggctctcg tttctactct cttagcggaa gacatgatat tgcgacctac cgaccttgag
     1501 acaccccacg ctctgcacat cgagcgcctg tccttctcct accccgacgg gcacccggcg
     1561 ctacacgatg tcacgctcga cgtgcggcag ggggagaagg tggcgattgt cgggccgaac
     1621 ggggccggta agtccaccct catcctgcac ctgaacggca tactcacggg gcagggcacg
     1681 gtgcgcgtgt gcgggctgga cgtggtcaag ccgaacctgg gcagggtgcg cgggctggtg
     1741 gggatggtgt tccagcttcc cgacgaccag cttttctcgc ccaccgtgtt cgacgacgtg
     1801 gcgtttggcc ccctctacca gggcttgccg gaggcggaag ttcggttgag ggtggaacag
     1861 gcgctggcgg cggtaggcat gagcgagtac gtgcaccggg tgtcgcacca cctgagcgtg
     1921 ggcgagaaga agcgtatcgc ggtggctacc gtgctgtcga tgcagcctga gatgctggtg
     1981 ctggacgagc cgtcggcggg gctggacccc cgtgcgcggc gcggcctcat caacctactg
     2041 cgcgacctgc cccagaccat cctggtgaca acccacgacc tgcccctggt gcgcgacctc
     2101 tgcccccgca ccatcatcat ggatggcggc accatcgtag cggacggccg cacggcggac
     2161 atcctcgcgg accaagcctt gctcaccgct cacgggttgg agatgcccta attccgcttt
     2221 ggcgcggata atcgggtttt acaaggggcg cgacgtatcg tgcccttgct gctttctgag
     2281 gattccagcc agccaattt
//
LOCUS       DASRWZ010000580         5448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_458762, whole genome shotgun sequence.
ACCESSION   DASRWZ010000580 DASRWZ010000000
VERSION     DASRWZ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458762"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..2346
                     /locus_tag="VFH60_09125"
     CDS             106..2346
                     /locus_tag="VFH60_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313985.1"
                     /translation="MTAPTPTVKAVPAEQVEENTEGAVDQDNARSSLRSGWFAAFFFL
                     IATVAYTWPLFTHMASTLPDALDSTDTARQLAEIARNLSTNPLHIYDSQGLYPLDNDL
                     ALNELLVAQGIYAAPIIWLTGNMLLAWNLVTFSSYVLSGLAAWLLVRKATGSSLAGLA
                     AGIIYAFSPWHYGQYGHLGIEAIHWMVFGLYFLALFLERTATSSRLLDRRGLLYLALF
                     TFFATLQPLSAGYYGYFEAILFGMYLLYFALRHSGAGGWAWSKLRRVPTSRVDWRRLG
                     GQVALLGLAALVALAVIYPFVRPYTHYKNLFGFNRGLEEVEYWSAGPLSLLRTTPTSW
                     WYEPVQKGIFGLETSAEREMYPGVAAVALALVGLFAASRVRKDREGRGFFAALVLVGL
                     ALSLGPTFHWDSYGLGDTGVPLPWYRILYDYVPGFDAVRVPHRFVLLAMLGIGALAGL
                     GIARLVSWSRDKGFSYPGMVGGAALLLVMLDFVAPGLRYITRGLGENAPPLYRWLAGA
                     EAASLIPADALLLELPVGLDKTPVNTSPQYLLYGVSHGRPMLNGSPNIIPPGYERLFS
                     EMRRFPTPGTLDIIEGLGVQFVVVHTGGLLNDEKRDELYRIAAEGTRLEQVADLPDVD
                     PFGQSAPHSKARVYRVKRAAGRFDRLRAAIPPGSSVLLADHPSKLRLTNTALPNLLGH
                     DRRYFTTYRTIYDPIVGPLQDATPGERYDFTVIYNEDDPASYGFEPEDRVDVGDIDLI
                     QVYRKR"
     gene            complement(2321..3262)
                     /locus_tag="VFH60_09130"
     CDS             complement(2321..3262)
                     /locus_tag="VFH60_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009633950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6313986.1"
                     /translation="MDVQTADQQAERMGLVFGFVGVLAFGLTLPATREAVAYFDPLLV
                     GMGRVVVASVLAAVLLLVTKQRWPSRPQWRGLVIVASGVGVGFPVLSAWALQEVPAAH
                     GSIVLGLLPLATALFGALRAGERPTRGFWAASALGSLTVVGFAFSRGAGQLQLEDLAL
                     VAAVIMAGLSYAEGGRLAVTMGGWQVICWALVLTGPFLVLPSAWALLEHGLAGPPGAW
                     LGFGYVSLVSQFLGFFAWYRGLALGGIARVGQLQLLQPFVTIGAAALLLGEAITPDVL
                     VAALVVVGTIALGRKAGVRRNLRETATPATTASGTPG"
     gene            3368..3928
                     /locus_tag="VFH60_09135"
     CDS             3368..3928
                     /locus_tag="VFH60_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET6313987.1"
                     /translation="MELGANIKRLRGELGLTLEALSERSGVSRAMLSDIERGLKSPTI
                     RVVSQVAEGLGCSVSRLLGEDAGPAQDGVQVLRRDERQVLVDPKSGVERHLLSPRFLR
                     RGLELVWYAIPPGQVTGAFPPHRPGTEEHITVVQGTLRCRISESDVTLQEGDSLSFRA
                     DLEHAFENAGSEECRYFLVIDSGSAR"
     gene            complement(4263..4703)
                     /locus_tag="VFH60_09140"
     CDS             complement(4263..4703)
                     /locus_tag="VFH60_09140"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6313988.1"
                     /translation="MSGFQMSEWIACPPKRVFEFITDSSNAPKIVPSVRSSVKLTEGP
                     PKVGTRYRETRLMGGKEQHAELEVSEYAEPELYAMRNVTDQIETVYRYKLHPEREGTR
                     IDLVCEIKSAGIKKLMLPLVALILKKEDGAHLQRLKQVLEEQTV"
     gene            complement(4723..>5448)
                     /locus_tag="VFH60_09145"
     CDS             complement(4723..>5448)
                     /locus_tag="VFH60_09145"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HET6313989.1"
                     /translation="TILQIALDAGFKTHESFTRAFKRQFGITPSEFRSNFLRISREHK
                     KHVQPKYISDCNMQDESGLLPNRSTSAQVRVEQVRSIIVAFVRHVGPYDKLLEKGSPM
                     APLWDELFHWGNENKLINAASLLIGLPQDDPSVTPPDKQRFDVCVQIPEFRNPSGHIG
                     CQTISAGTFGVGRHYGSFDNLAETYMHVYDSLVTTGKYRLRDHIPFEVYSYSHVKDDI
                     RIHFTDVYLAIEPTDPEKLTKER"
BASE COUNT         1036 a         1713 c         1657 g         1042 t
ORIGIN      
        1 acgggcgact gatccttcgc tccgctcagg atgacaaggg ggatatggta acgctaccca
       61 acctggtcgc tgcctacgcg cctgtatgct ataatcgcgc aagccatgac agcacccaca
      121 cccaccgtaa aagcagtccc agccgagcag gtagaggaga atacggaggg ggcagtagat
      181 caagacaatg cgcggtcgtc cctccggtcg gggtggttcg ccgccttctt cttcctcatc
      241 gcgacggtgg cttacacgtg gcccctgttc acccacatgg ccagcacgct gcccgacgcc
      301 ctcgacagca ccgacacggc ccgccagctt gccgagatag cccgcaatct cagcaccaac
      361 cccctccaca tctacgacag ccagggcctc taccccctcg acaacgacct cgccctgaac
      421 gaactgctcg tcgcgcaggg catatacgct gcgcctatca tctggctgac gggcaacatg
      481 ctgctggcct ggaacctggt caccttctcg tcgtacgtgc tgagcgggct ggcggcctgg
      541 ttgctggtgc gaaaggccac gggcagcagt ctggcggggc tggcggcggg catcatctac
      601 gccttctccc cctggcacta cgggcagtac ggccacctgg gtatcgaggc gatccactgg
      661 atggtgttcg gcctctactt cctggcgctg ttcctggagc gtaccgccac cagctcgcgc
      721 ctgctggacc ggcgcggact gctctacctg gcgctgttca ccttctttgc caccctgcaa
      781 cctctctcag cagggtacta cggctacttt gaagccatcc tatttggcat gtacctgctc
      841 tactttgcac tgcggcactc cggtgcgggc ggctgggcgt ggagcaagct gcgccgggta
      901 ccgacatcgc gagtggactg gcgcaggctg ggcgggcagg tcgcgctgct gggccttgcc
      961 gccctggtgg ccctggcggt catctaccct ttcgtgcggc cgtacacgca ctacaagaac
     1021 ctctttggct tcaaccgcgg gctagaggag gtggagtact ggagcgcggg gccgctgagc
     1081 ctgttgcgca ccacccccac aagctggtgg tacgaacccg tgcagaaggg catattcggc
     1141 cttgagacca gcgccgagcg cgagatgtac cccggcgtcg cagcggtcgc gctggcgttg
     1201 gtggggctgt ttgccgcctc ccgcgtgcgg aaggacaggg agggccgtgg gtttttcgct
     1261 gcgcttgtgc tggtggggct ggcgttgagc ctcggcccca ctttccactg ggactcctat
     1321 gggcttggtg acacgggcgt tcccctgccg tggtacagga ttctgtacga ctacgtgccg
     1381 gggttcgacg ccgtgcgggt gccccaccgt tttgtgctgc ttgccatgct cggtatcggc
     1441 gcgctggcgg gactggggat cgcgcgcctg gtgagttgga gtcgggacaa aggcttctcc
     1501 tatcccggca tggtgggcgg cgcggccctc ctgctcgtga tgcttgattt cgtcgcgcct
     1561 gggttgcgct acatcacgcg agggctggga gagaacgccc cacccctcta caggtggctg
     1621 gcgggggccg aggcagccag cctcataccg gccgacgctc tgctgcttga actgcccgtg
     1681 gggctggaca agacgccggt gaacaccagc ccgcaatacc tgctctacgg cgtgtcccac
     1741 gggcggccca tgctcaacgg cagccccaac atcatcccgc ccggctacga gcgcctgttc
     1801 tccgagatgc gccgcttccc tacccccggt acgcttgaca tcatcgaagg gctgggcgtg
     1861 caattcgtgg tggtgcacac aggggggttg ctgaacgacg agaagcgcga tgagttgtac
     1921 cgcatcgcag ccgaaggcac caggctggag caagtcgcgg acctgcccga cgtagacccc
     1981 ttcggccagt ccgctcccca ttctaaagcg cgggtctacc gcgtcaagcg ggctgcgggt
     2041 cggttcgaca ggctgagagc cgccatcccc ccaggctcct cggtgctgct ggccgaccac
     2101 ccctcgaagc tgcgccttac taacaccgcc ctccccaacc tgctgggcca cgaccgccgc
     2161 tacttcacca cctaccgcac catctacgac cccattgtcg gcccgctgca agacgccacg
     2221 cccggcgaac ggtacgattt cacggttatc tacaatgagg acgaccccgc cagctacggc
     2281 ttcgagcccg aagaccgtgt ggacgtaggc gacatcgacc tcatccaggt gtaccggaag
     2341 cggtagtcgc gggtgtggcg gtctcccgca ggttgcgccg cacccctgcc ttcctgccca
     2401 gggctatagt ccctaccacg accagggcgg cgacgagcac gtccggtgtt attgcttcgc
     2461 caagcagcag ggcggcggca cctatagtta caaacggttg cagtagctga agctgtccca
     2521 ccctggcgat accgccgagg gcaagcccgc gataccaggc gaagaagccg aggaactggc
     2581 tcaccaggct gacgtaaccg aagccgagcc aggcccctgg cggcccggca agcccgtgct
     2641 cgaggagcgc ccaggccgaa ggcaacacca ggaaggggcc ggtcagcacc agcgcccagc
     2701 agatcacctg ccagccgccc atcgtgacag ctaatcgtcc gccctctgcg tagctgagtc
     2761 ctgccattat cacggcggcc accagcgcca ggtcctccaa ttgcagttgc cccgcgcccc
     2821 ggctgaaggc gaagccgaca accgttaagc tgcctagcgc actcgccgcc cagaagcccc
     2881 tggtcgggcg ctcacccgct ctgagagcgc cgaataaggc ggtggcgagg ggcaggaggc
     2941 ccagcacaat ggagccgtgc gcggcgggta cctcctgcaa ggcccacgcg gagagtaccg
     3001 ggaagccgac ccctaccccc gaagccacaa tcacgaggcc gcgccactgc gggcgagagg
     3061 gccaccgctg cttagtcacc agcaacaaca cggccgcgag cacggaggcc accacgaccc
     3121 ggcccatacc taccagcaac gggtcgaagt aagccacggc ttcccgtgtc gcgggcagtg
     3181 tgaggccgaa agccagcacg ccgacgaagc caaataccag ccccatacgc tcagcttgct
     3241 gatctgccgt ttgcacgtcc atcttgccct cttggcgtta tagtggaagt ttgtctatta
     3301 tagtagacaa gaataagggt gtcaactata gcgatgacct gctacaatag cggaaaaggt
     3361 ggtacatatg gagctgggcg cgaacataaa gcggctgcgg ggcgagctgg ggctgacgct
     3421 ggaagcccta tcggagagga gcggggtcag ccgggccatg ctctcggata tagagcgcgg
     3481 cctcaaaagc cccaccatcc gagtagtgtc gcaggtagcc gaggggctgg gttgtagcgt
     3541 gtcgcggctg ttgggagagg acgctgggcc ggcccaggac ggggtgcagg tgctcaggag
     3601 agacgagcgg caggtgctgg ttgacccgaa atcgggcgtg gagaggcacc tactttcccc
     3661 ccgcttcctg cgccggggcc tcgaactggt atggtacgcc atcccgcccg gccaggtaac
     3721 cggcgcgttc ccgccccacc gccccggcac ggaggagcac atcacggtcg tccagggcac
     3781 cctccgctgc cgcatcagcg agtccgacgt caccttacag gaaggcgact cgctctcgtt
     3841 tcgcgccgat ttggagcacg cattcgagaa cgcaggcagc gaagagtgca ggtatttcct
     3901 ggtaattgac tccggcagcg ccaggtgacg cggaggctac ggcatagatt tcagcgtcag
     3961 agatgggggc gggagggtgc ctgctactac tattccgcgc tgctatccgg tctacgtggt
     4021 gaacttacaa tactaacgcc catggcgggg cgtgggcctc ataggctgct atccggcctg
     4081 gaagagtgaa ttgttgttgt tcctggccgg aatctactgg aaggagtaaa gctcagaacg
     4141 ttgatcgcta aagtaggggc gcaacgggat attaatggtc aacgtacagg ggtataattg
     4201 cccataaaaa agtgccccac acgtgtgggg cacccggcgt accggtaaac tctccagcct
     4261 tcttatacag tctgttcttc caagacctgc ttcaaccttt gcaaatgcgc tccgtcctcc
     4321 tttttcaaaa tgagtgcgac caggggaagc attagcttct tgatccccgc ggatttaatt
     4381 tcgcaaacca ggtcaatgcg cgtgccttcc ctttcaggat gtaatttgta ccggtagacc
     4441 gtctcgatct gatcagtcac gtttcgcatg gcgtataact cgggctcggc gtattcgctc
     4501 acttccagct cggcatgctg ttctttgccg cccatcaagc gggtctcgcg gtagcgggtg
     4561 cccaccttcg gaggcccttc cgtcagcttg accgagcttc ttaccgaggg gacaattttg
     4621 ggggcgttgc tcgaatcggt aataaactcg aaaacgcgct tcggtgggca ggcgatccat
     4681 tcactcattt gaaatccgga cattttattc tccttgataa agttagcgtt ctttggttag
     4741 tttttccggg tcggtcggct cgatagccag ataaacatca gtaaaatgga tgcggatgtc
     4801 gtccttgaca tgcgaataac tgtacacttc gaaaggtatg tggtctcgga gccggtattt
     4861 gcccgtggtg accagcgagt catacacgtg catgtacgtc tctgccaggt tatcgaaaga
     4921 accgtaatgc ctgcccaccc caaaggtccc ggccgaaatc gtctggcagc cgatgtggcc
     4981 cgaagggttg cggaattcgg ggatctgtac gcacacatca aagcgttgct tgtcaggggg
     5041 tgtgaccgac gggtcgtcct ggggcagccc gattaacagc gaagcggcgt tgatcagttt
     5101 attttcattc ccccagtgga ataactcatc ccagagcggg gccataggcg agcctttttc
     5161 cagcagcttg tcatatgggc cgacatgacg gacaaaagcg acgatgatcg aacgcacctg
     5221 ttccacgcgg acttgcgcag aggtggaacg gttcgggagc aaacccgatt catcctgcat
     5281 gttgcaatcc gaaatatatt tgggctgcac atgctttttg tgttcacgcg atatgcgcag
     5341 gaaattactg cggaactcac tcggcgtaat accaaactgc ctcttgaatg cgcgcgtgaa
     5401 agattcatgc gtcttgaacc cggcatccag cgcaatctgc aagatcgt
//
LOCUS       DASRWZ010000581         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_801295, whole genome shotgun sequence.
ACCESSION   DASRWZ010000581 DASRWZ010000000
VERSION     DASRWZ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801295"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..>1864
                     /locus_tag="VFH60_09150"
     CDS             83..>1864
                     /locus_tag="VFH60_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET6313990.1"
                     /translation="MFTFGVDYYPEHWPEYRWPTDAKLMAEAGFNITRLAEFAWSRLE
                     PQEGTFDFAWLDRAIDVLAGQGIRVILGTPTASPPPWLMEAHLDLYRVDEQGRRLTFG
                     NRREYCPNHPLYHERTRAIVTAMAEHYAEHPAVVGWQIDNEFGDPCYCPVCREAFQGW
                     LRERYGTLDALNSAWGTIFWSHVYTDWPQIGVPLASGGAPNPGLALDFSRFASDSYVR
                     YQQLQIDLLRARTSGQFITHNLMGFKYANLDYFDLARPLDFVTWDNYPRTQWSLNANV
                     DPSGAALAAATMRGLKRAPFWVMEQQAGPGGWDLVSVMPRPGELRLWAYQQIAHGADG
                     IVFFRWRTGRHGTEQYWHGLLDHDATPGRRYTEIARMGAELGRMGEALAGTEVRAPVA
                     MLLSYDSRFAFHIQPNNPGYSYAEHFHDIYRSFHARNVPVDVLAPTDDLGGYQLVLAP
                     PLYVLPAAVAANLESYVRAGGTLVCFFRSGVKDEANAVVNRRLPGLLAEMCGVEVAEY
                     DSLPPGVQNGLEWSLPGVAGAASGACRAWADILAPTTAEVIARYTSDYYAGQAAVTLN
                     RYEAGSVVYVGIVGDAATHATLVDWLIA"
BASE COUNT          321 a          603 c          602 g          338 t
ORIGIN      
        1 ctcttgggtg ctgataattc caacctgtga gattttgctt tcgccctatc ggtaacactc
       61 tatcgagtcg agaggaccgc acatgttcac ctttggcgtt gactactacc ccgagcactg
      121 gcccgaatat agatggccca cagacgcaaa actcatggcc gaggcgggct tcaacatcac
      181 ccggctggcc gagttcgcat ggtcgcggct ggagccgcag gagggcacgt tcgatttcgc
      241 ctggctggat agggcgatag acgtgctggc cgggcagggc atacgggtga tactgggcac
      301 tcccacggcc tcgcccccgc cctggctgat ggaggcccat ctcgacctct acagggtgga
      361 cgagcagggg cggcggctta ccttcggcaa ccggcgcgag tactgcccaa accaccccct
      421 ctaccacgag cgcacgcggg ccatcgtgac cgccatggcc gagcactacg ccgagcaccc
      481 ggcggtggtc ggctggcaga tagataacga gtttggcgac ccttgctatt gccccgtttg
      541 ccgggaggcg ttccagggct ggctgcgcga gcgttacggc acgctggacg ccctcaacag
      601 cgcctggggc actatcttct ggagccatgt ttacaccgat tggccgcaaa tcggcgtacc
      661 gctcgccagc gggggtgcgc ccaaccccgg cctggcgctg gatttcagcc gcttcgcctc
      721 cgactcctac gtccgctacc agcagcttca gattgacctc ctgagggcgc gcacctccgg
      781 gcagttcatc acccacaacc tgatgggctt caagtacgcc aacctcgact atttcgacct
      841 ggcgcggccc ctggactttg tgacgtggga caactacccg cgcacgcagt ggtctctcaa
      901 cgccaacgtg gacccaagcg gggctgcgct ggccgcggcc accatgcgcg ggctgaagag
      961 agcgccgttc tgggtgatgg agcagcaggc ggggccgggc ggctgggacc tggtcagcgt
     1021 gatgccacgg ccgggggagc tacggctatg ggcctaccag cagatcgcac acggggcgga
     1081 cggcatcgtc tttttccgct ggcgcacggg gcggcacggc accgagcaat actggcacgg
     1141 cctcctggac cacgacgcca cgccgggccg ccgctacacg gagatagcgc gaatgggcgc
     1201 ggagctggga cggatgggtg aggcgctagc aggcacagag gtccgggcac ccgtggcgat
     1261 gctcctctct tacgactcgc gctttgcctt ccatatccaa ccgaacaatc ccggctacag
     1321 ctacgcggag cacttccatg acatctaccg ctcgttccac gcgcgtaacg ttcctgtgga
     1381 cgttttagcg cccaccgacg acctgggcgg ctaccaactg gtgcttgcgc caccgctgta
     1441 cgtgcttccc gcagccgtag cggccaacct cgaaagctac gtgcgggctg gcggcaccct
     1501 ggtgtgcttc ttccggtcag gggtgaaaga cgaggccaat gcggtggtca accggcggct
     1561 gcccggccta cttgccgaga tgtgcggtgt ggaagtagcc gaatacgact ctctcccgcc
     1621 gggagtgcag aacgggcttg aatggtccct tccaggtgtg gcgggtgcag cttcgggcgc
     1681 atgccgggca tgggccgaca tcctggcccc gactaccgcc gaggtaatag cgcgttacac
     1741 gtcggattat tacgcgggac aggcagcggt taccctcaac cggtacgagg ccggtagtgt
     1801 cgtgtatgtc ggtattgtgg gggatgccgc cactcatgcg acccttgttg attggctgat
     1861 cgcg
//
LOCUS       DASRWZ010000582         4069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_391297, whole genome shotgun sequence.
ACCESSION   DASRWZ010000582 DASRWZ010000000
VERSION     DASRWZ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391297"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..1339
                     /locus_tag="VFH60_09155"
     CDS             305..1339
                     /locus_tag="VFH60_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020006937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313991.1"
                     /translation="MTRIYMAAGDAFAVIEGREGRWRASVTLQGLHTTCVEVDPLKPE
                     RVYCGTFGRGLWVSDDQGAGWRQAGEGIAHAEVTAVAASATERYAGYGVVWVGTEPSA
                     IFRSEDGGNSWVERRSLQDLPSRPTWSFPPRPWTHHVRWIAPDPLVPERLFVGIELGG
                     VMRSLDGGLTWEDRKPEAQPDAHHLRTHPLAPGRVYETAGGGYAESLDGGATWRRDDA
                     GMRHHYLWGLAVDPADAHTLLVSASRGPMSAHRREAAEARMYRKVAGQPWTEVRDGLP
                     QAEGTRVYGLASNPDEPGVFYAATHEGGVYRSADSGLTWHALDVDWPQGTHIDSQMPV
                     ALAVIGAGQG"
     gene            1523..2506
                     /locus_tag="VFH60_09160"
     CDS             1523..2506
                     /locus_tag="VFH60_09160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6313992.1"
                     /translation="METTNAFTGRPYTEADLPAISELINYIDSVEHLDENATPEDLRQ
                     WFTSPSIDPQKNIRVWEDGNGRLVAVAVVMTLAPTETSSDGRLIFYIHPDVPRSGLDS
                     ELLEWAEGRLREHGAAHGVPCNLYSGAQESYGSRREALERNGFTIARYFFVMHRPLDE
                     PLEEPTFPKGFTLSNVAEHPDIEKYVEMHNLAFIDHWSFHPMLPERRAHTLKDPHYNP
                     EGGLIALAPDGTFAAFADCYIDAGFNERAGKSEGWIGTLGTRRGYRKMGLGRAMLLAG
                     LHWLKSQGLQTGVLDVDAENPTGALRLYESAGFRTVKTEVVYCKEVASNED"
     gene            2640..2930
                     /locus_tag="VFH60_09165"
     CDS             2640..2930
                     /locus_tag="VFH60_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313993.1"
                     /translation="MQKMLWMNGPAEITIEECIEFVNHFLWNLSVVHGENGQIWVLTG
                     DQAIFYADNTDAVEAFLYGMGLAYRVLPAEMAIDLGKSMGFSEEEIKEMQHP"
     gene            3146..3514
                     /locus_tag="VFH60_09170"
     CDS             3146..3514
                     /locus_tag="VFH60_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET6313994.1"
                     /translation="MRTLLVRWAVIVIAVFLVAWGLPQIGLTGDPIIRYDDWLTLAIF
                     AAVLALLNTFIRPIVGMLALPINCLTFGLFSIVINAAMFALAAALVPGVHVADFWAAL
                     VGAVAVSVVGALANMITGAR"
     gene            3543..>4069
                     /locus_tag="VFH60_09175"
     CDS             3543..>4069
                     /locus_tag="VFH60_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6313995.1"
                     /translation="MLLDHIVILVDDLDLASRDYTALGFSVVVGGKHADGLTQNSLVA
                     FEDGSYLELLNFLSPPPDEHLFARGAQSAEGIVAYALLPDDIDDDIARARRHGLHIRG
                     PIRGGRLRPDGLEIAWQTGRIDHPALPFLCDDLTPRDLRVPHGPAQRHPNGVTGIAEV
                     EVLVEDLDEAARCYTA"
BASE COUNT          790 a         1290 c         1281 g          708 t
ORIGIN      
        1 gttggtttgt ggctaagagc cttttggcct tgggcggccg aagccccgcc tttgtcattc
       61 tgagcgcagc gaagaatctc gtccatcagc acccaaatac cacctttctt caggcgaacg
      121 tgtccggtca agaaaggtgt accatcccgg tgcaggacga gattcttcgc tacgctcaga
      181 atgacagagg ggggcctggt cctgcgatag cgcctttgtt gaatccaagc agcaataata
      241 gcaaggcata ttgcgagtcg cgcaaagaat tacgtgttac gcgttagtca aaggtgaggc
      301 acacatgacc cgcatctaca tggccgccgg tgacgcattt gccgtgatag aggggcgcga
      361 ggggcgttgg cgggcgagcg tcaccttgca ggggctgcat acgacctgcg tagaagtgga
      421 cccgctgaag ccggagcgtg tgtactgcgg cacttttggg cgggggctgt gggtcagcga
      481 cgaccagggc gctggctggc ggcaagcggg agagggcatc gcccacgccg aggtaacggc
      541 ggtggcggcg agcgcgaccg aacgttatgc cggttacggt gtggtgtggg tgggcaccga
      601 gcctagcgcc atcttccgct cggaggacgg gggtaatagc tgggtggagc ggcgcagcct
      661 gcaagacctc ccttcgaggc caacctggag cttcccgccc cggccctgga cgcaccacgt
      721 ccgctggata gcccccgacc ctcttgtgcc tgagcgcctg ttcgtgggca tcgagcttgg
      781 cggcgtgatg cgcagcctgg acggcggcct cacctgggag gaccgcaagc cggaggccca
      841 gcccgacgca caccacctgc gcacccaccc tctcgcaccc ggcagggtgt acgaaacggc
      901 gggcggcggc tacgcggaga gcctcgatgg cggggccacc tggcggcgcg atgacgcggg
      961 catgcggcac cactacctgt ggggcctggc ggtcgatccc gccgacgccc acaccctatt
     1021 ggtctcagcc tcaagaggcc ccatgagcgc ccaccggcgc gaggcagccg aggccaggat
     1081 gtaccgcaag gtagcggggc agccctggac cgaggtgcgc gacggccttc cccaagccga
     1141 gggcacccgc gtttacggcc tcgcctcgaa ccccgacgag cctggcgtgt tctacgccgc
     1201 aactcatgag ggcggcgtct atcgctctgc ggatagcggc ctcacctggc acgcactgga
     1261 cgtagattgg ccccagggca cccacatcga tagccagatg ccggtcgcgc tagccgtgat
     1321 aggagcgggg caggggtagc ggttcgtgcc ccgttcgccc aatacatcca tcgtacgcgg
     1381 caagctacat ctgcggtccg atgctccccg gcagcgcggg tgatacgata acctacggcg
     1441 gaccacagcc aaccctcaat tgccgcctgt tgcctgtggt ccgccgttcc tatttaaccc
     1501 cacgaaagga caacgtgcta ttttggagac gactaacgcc ttcaccggac gaccctacac
     1561 cgaagccgac ttacctgcca tctcagagct tatcaactac atcgacagcg tagagcacct
     1621 ggacgaaaac gccacccccg aagacctgcg ccagtggttc acctccccaa gcatcgaccc
     1681 acagaagaac atccgcgtgt gggaggacgg caacggcagg ctcgtagccg tggcggtggt
     1741 catgaccctg gcacctaccg agacctcgtc ggacggacgg cttatattct acattcaccc
     1801 cgatgttccc cgaagcggcc tcgacagcga gctattggag tgggcggagg gcaggctgcg
     1861 agagcatggg gccgcgcatg gcgtgccctg caacctgtac agcggcgcgc aagaaagcta
     1921 cggtagccgg cgggaggcgc tggagcgcaa cggcttcacg atagcccgct acttcttcgt
     1981 gatgcaccgc cccctggacg agccgctgga ggagcctacg ttcccgaaag gcttcactct
     2041 cagcaacgtg gccgagcacc cggacataga gaagtacgtc gagatgcaca acctggcctt
     2101 cattgatcat tggagcttcc acccgatgct ccccgaacgc cgcgcccaca cgctgaaaga
     2161 cccccactac aacccggagg gaggtctcat cgccctcgcg cccgacggca ccttcgccgc
     2221 tttcgctgac tgctatatcg atgcaggctt caacgagcgt gcgggcaaga gcgaaggctg
     2281 gataggcacg ctgggcaccc gccggggcta ccgcaagatg ggcctcggac gcgccatgct
     2341 cctcgcgggg ctgcactggc taaaatccca gggcctccag accggcgtgc tcgacgtgga
     2401 cgccgagaac cccaccgggg ccttgcgcct gtacgagtcg gccggcttcc gcacggtcaa
     2461 gacggaggta gtgtactgca aggaagtggc gagtaatgag gactgaggaa tgaggactga
     2521 gttcccattg cagattgtag gctgtggtaa gtaggcgagg agggggcggg ggtgccctgc
     2581 gatagccctg tggctaaagc cacgggctgc agaacaccag ccgtacggag ggcaacgtcg
     2641 tgcagaaaat gctatggatg aatggtcccg ctgaaatcac aattgaggag tgtatagagt
     2701 ttgtcaatca cttcttatgg aacctatcgg tagtccatgg agaaaatggg cagatatggg
     2761 tgctgacagg tgaccaagct attttctatg ctgataacac agatgccgtt gaggcattct
     2821 tgtacgggat gggcctggca tacagggtac ttcccgcaga aatggcaata gacctgggca
     2881 aaagcatggg tttcagtgag gaagaaataa aggagatgca gcacccgtag gttttgttag
     2941 gtgccgtaag gaacaagagg gtagtctccg aaggagactt cgctacttcc cagcccgtgg
     3001 cttcagccac agggcatccc cacagagcga tggtgatacg ccgctactcg ccactcagtc
     3061 ctcagtcctc agtcctcatt actcctccct cctatgctat aatggaccgg ccaagcgtcg
     3121 agtgtagcca cacaggagga gagaattgag aacgctgcta gtccgttggg cggtcatcgt
     3181 gatcgcggtg ttccttgtag cgtggggcct gccccagata ggactaacgg gcgacccgat
     3241 catcaggtac gacgactggc tgacgctggc gatcttcgcg gcggtgctgg ccctcctcaa
     3301 caccttcatt cgccctatcg tgggcatgct ggcgctcccg ataaactgcc tcaccttcgg
     3361 cctcttcagc atagtcataa acgcggccat gttcgcgctg gcggcggccc tggtgccggg
     3421 cgtgcacgtg gccgacttct gggccgcgct cgtgggtgcg gtggccgtca gtgtggtggg
     3481 cgcgctggct aacatgatta ccggggcgcg ctagcccgcc acctctgccc cctgccactg
     3541 ccatgctgct cgaccatatc gtcatcctcg tggacgacct agaccttgcc agccgcgact
     3601 acaccgcgct cggcttctcg gtagtagtcg ggggcaagca cgcggacggc ctgacccaga
     3661 actcgctcgt ggccttcgaa gacggctctt acctggagct gctcaatttc ctctcgcccc
     3721 cgcccgacga gcacctgttc gccaggggcg cgcagtcggc tgaaggcata gtcgcttacg
     3781 ccctcctacc cgacgacata gatgacgata tagcccgcgc gaggcgtcac ggcctgcata
     3841 ttcgcggccc catcaggggc ggtaggctgc ggccggacgg cctggagatc gcctggcaga
     3901 ccggccgcat cgaccacccc gccctcccct tcctgtgcga cgacctcacc ccacgcgact
     3961 tgcgagtgcc ccacggcccg gcccagaggc accccaacgg cgtgaccggc atagccgaag
     4021 tcgaggtgct cgtggaagac ctggacgaag cggcccgctg ctacaccgc
//
LOCUS       DASRWZ010000583         1544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_596326, whole genome shotgun sequence.
ACCESSION   DASRWZ010000583 DASRWZ010000000
VERSION     DASRWZ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596326"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFH60_09180"
     CDS             complement(<1..641)
                     /locus_tag="VFH60_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6313996.1"
                     /translation="MGFFDKLFGRQEQPEQPRPAQQPYQGGQYNQSQYGQQGQQYGQQ
                     QYGGQYGQQPGQPGQPGQMTDEQAVARYRYMLRTAPPETIEQAHAEAFAQLTPEQRQM
                     VLQELSSNLPEHERRAAQYGDDPQSLARMATRAEMRQPGTMERTFSGMGAGGGGGVMG
                     GGIGMGGLMAGTFFSSFAGMMLGGMIAQSFFMNDHGYSDGYADGQAAGDQNADG"
     gene            832..1500
                     /locus_tag="VFH60_09185"
     CDS             832..1500
                     /locus_tag="VFH60_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6313997.1"
                     /translation="MEGKGEERVVRVAVGAVTLEGNMVVPPGARGVVLFAHGSGSSRH
                     SPRNRYVAGVLQEVGLATLLVDLLTQQEEQVDVRTAHLRFDIGMLAERLAGATDWLAQ
                     QPETADLRVGYFGASTGGGAALVAAAERPEAVGAVVSRGGRPDLAGPALPRVSAPTLL
                     LVGGNDPIVIELNEQAKARMRGEVQLTIVPGASHLFEERGTLEEVARLARDWFLRYLP
                     THQQ"
BASE COUNT          249 a          474 c          543 g          278 t
ORIGIN      
        1 ccgtcggcgt tctggtcgcc cgcggcctga ccatcagcgt agccgtctga gtagccatga
       61 tcgttcatga agaacgactg ggctatcatg ccgccgagca tcatgccggc gaagctgctg
      121 aagaaagtac ccgccatcag cccgcccatg ccgatgccgc ctcccatgac gcccccgcca
      181 cccccagcgc ccatgccgct gaaggtgcgc tccatcgtgc cgggctggcg catctcggcg
      241 cgagtagcca tgcgtgcgag ggactggggg tcgtcgccgt actgggcggc cctgcgctcg
      301 tgctccggga ggttgctgct aagttcctgc aacaccatct ggcgctgctc aggggtaagc
      361 tgggcgaagg cctcggcgtg ggcctgctcg atagtctcag ggggcgcggt gcgcagcatg
      421 tagcggtagc gggcgaccgc ctgctcgtcg gtcatctgtc caggctgccc aggctgtccg
      481 ggctgctgcc cgtattggcc gccgtactgt tgctggccgt actgctggcc ctgctggccg
      541 tactgcgact ggttgtactg tccgccctgg tacggctgtt gcgcggggcg tggctgctcc
      601 ggctgctctt gccgtccgaa tagcttgtca aagaatccca tgatgtgttt cctccgttac
      661 gtcaggttgt ctcctggcta agcgccgcgg tcacggcaac cttatatagc tacgaagcaa
      721 attgcttgcc agttgcattt ggacgatgga ctttagacta tggttcggcc ccatttatgc
      781 tgataaaggg gtgtgattat ttggtagccg ggagaaaggc aggaggcgaa aatggaagga
      841 aagggtgagg agcgggtggt gcgggtagcg gtgggggcag ttacgctgga gggcaacatg
      901 gtggtgccgc ccggcgcgag gggagtggtg ctgttcgctc acggcagtgg tagcagcagg
      961 cacagcccgc gcaaccgcta cgtggccggg gtgttgcagg aggtgggcct cgccacgctg
     1021 ctggtggacc tgctgacgca acaggaggag caggtggacg tgcgcacggc ccacctgcgc
     1081 ttcgacatag gcatgctcgc cgagcggctg gcaggggcta ccgactggct cgcccagcag
     1141 cccgagacgg ccgatttgcg ggtgggttac ttcggcgcga gcacaggcgg gggtgccgcg
     1201 ttggtggctg ccgccgagcg gcccgaggcg gtgggggcgg tggtatcgcg cggcgggcgt
     1261 cccgacctgg caggccccgc cctacccagg gtcagcgcgc ctaccctgct gctggttggg
     1321 ggtaacgacc cgattgtaat cgagctgaac gagcaggcga aagcccggat gcggggcgag
     1381 gtgcagctta ccatcgtccc cggcgcgagc cacctattcg aggagcgcgg cacgctggag
     1441 gaagtagccc gcctcgcccg cgactggttc ctccgctacc tccctaccca ccaacaatga
     1501 aacgagtcta aacgccaaga cgccaagacg ccaaggacca cgcc
//
LOCUS       DASRWZ010000584         1865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1144253, whole genome shotgun sequence.
ACCESSION   DASRWZ010000584 DASRWZ010000000
VERSION     DASRWZ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144253"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1653
                     /locus_tag="VFH60_09190"
     CDS             <1..1653
                     /locus_tag="VFH60_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6313998.1"
                     /translation="DEVYQPVLVPLEGGFPEPAFGSELEGYQVFALKCFADRNLVYLL
                     AASTTESIFKAYRGNLETGELEFMAESQYGGFIDSVGNDHTRAILIDAYSGGEHVLYL
                     WREGQGRELLYGAPPDRRNEQSPPAINTIGASQFTEGDRGLLFSTSLFEDAFGLGYMR
                     LDDPSGVKPVAVEGTRHTGTGELANLEHLHGNRYLVEYNIDGASWLYEGTFDEDARRM
                     TLDTVVCGQAELSGGVKQGHHYDKEGDRFVLSFSTAASPTQLYTVEGKERRVVAHTQE
                     RVLGVSPGWLSQGEDASFASFDGLRVSARLYLPASELGYEGRRPLIYYVHGGPQSQER
                     PNFAWFSMPLIQFFTLNGFAVFVPNVRGSSGYGLDYTKRVERDWGGKDQRDHVYAMTE
                     VLPKDERLDTSRAGVMGRSYGGYMTLSLASRHPELWKAAVDMFGPYNLLTMIDRVPAT
                     WRPFMAHLVGDPEKDHDMLVERSPRTYLHQITAPLLVLQGANDPRVIERESRELVEEL
                     KGMGKQAEIIVFEDEGHDVLKYPNKVRCYNTIVEFFTEHLRP"
BASE COUNT          369 a          571 c          617 g          308 t
ORIGIN      
        1 gacgaggtct accagcccgt gctcgtgccc ctcgaaggtg gcttccctga gcctgcgttc
       61 ggcagcgagt tggagggcta ccaggtgttc gcgttgaagt gcttcgcgga ccgcaacctg
      121 gtgtacctgc tggcggcctc gaccacggag tcgatcttca aggcgtaccg gggcaacctc
      181 gaaacgggcg agcttgagtt catggcggag agccagtacg gaggcttcat cgactcggta
      241 ggcaacgacc acaccagggc catactgata gacgcctact ccggcggcga gcacgtgctg
      301 tacctgtggc gggagggcca gggacgcgag ctattgtacg gtgccccgcc cgaccggcga
      361 aacgagcaat cgccgcccgc catcaatacg ataggcgcca gccagttcac cgagggggac
      421 aggggcctcc tcttcagcac ctcgctgttc gaggacgcgt tcggcctcgg ctacatgcgc
      481 ctggacgacc cgtctggcgt caagccggtg gcggtagagg gcaccaggca caccggcacc
      541 ggcgagcttg ccaacctgga acacctgcac ggcaaccgct acctcgtgga gtacaacatc
      601 gacggcgcgt cctggctgta tgaaggcacg tttgacgagg acgcccgcag gatgacgctt
      661 gatacggtgg tgtgcggtca ggctgagctt tcgggcgggg tgaagcaggg gcaccactac
      721 gacaaagagg gcgacaggtt cgtgctgtcc ttctccacgg cggcctcccc cacccagctt
      781 tacacggtag aagggaagga gcggcgtgta gtggcccaca cccaggagcg ggtgctgggc
      841 gtgtcgccgg ggtggctgtc gcagggcgag gatgcgtcgt tcgccagctt cgacgggctg
      901 cgggtgtcgg cgcggctcta cctacctgca agcgagctgg gatacgaggg ccggcgaccc
      961 ctcatctact acgtgcacgg cggcccgcag tcccaggagc ggcccaactt cgcctggttc
     1021 tccatgcctc tgatccagtt cttcacgctg aacggcttcg cggtgttcgt gcccaacgtc
     1081 aggggcagca gcggctacgg gttggactac accaagcggg tggagcgcga ctggggcggc
     1141 aaagaccagc gagaccacgt ctacgccatg accgaagtgc tgcccaaaga cgagcgcctc
     1201 gatacgagca gggcgggcgt gatggggcgc agctacggcg gctacatgac gctgagcctc
     1261 gcctcccggc accccgagct gtggaaggcg gcggtagaca tgttcgggcc ttacaacctg
     1321 ctcaccatga tcgaccgcgt gcccgctacc tggcggccat tcatggcaca cctcgttgga
     1381 gaccctgaga aggaccacga catgctggtc gaacgctccc cccgtacgta cctgcaccag
     1441 ataaccgccc cgctgctcgt attgcagggt gccaacgacc ctcgcgtcat agaacgggag
     1501 tcgcgggagc ttgtcgaaga gttgaagggc atgggcaagc aagccgagat catcgtgttc
     1561 gaggacgaag ggcacgacgt gctcaagtac cccaacaagg tgcggtgcta caacacgata
     1621 gtggagttct tcacggagca cctgcggccg taggtccgtc cgcgcaaggc aaaggggata
     1681 ctgtaggggg tagcatcggc ctgaatattc ctgttcaact agtgccgtgc taactaagga
     1741 gtggcagttg agaaggagag caaccggcga tggttgaact gtaacgataa cagagaagcc
     1801 aacacaaggt gccagccgac gccccgcgat tgaaatcggg ggctacacct tgccaagtcc
     1861 ccctt
//
LOCUS       DASRWZ010000585         1986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_321813, whole genome shotgun sequence.
ACCESSION   DASRWZ010000585 DASRWZ010000000
VERSION     DASRWZ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321813"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1842)
                     /locus_tag="VFH60_09195"
     CDS             complement(<1..1842)
                     /locus_tag="VFH60_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6313999.1"
                     /translation="MATVAEIFETLDYGPAPEAKEPALQWIERHQPFGLFIDGEWVVP
                     GSGKYFDSQDPATGKSLAKVALGTAEDADRAIAAARRAFESWGATPGHVRARYMYAIA
                     RLLQKHSRLLAVLESLDNGKTIRETRDIDIPLVARHFYYHAGWAQLMETELPGMVPVG
                     VVGQIIPWNFPLLMMAWKIAPAIAMGNTVVLKPASYTPLTALAFAHICDEAGLPKGVV
                     NILTGEARTAGEAILTHPGVDKIAFTGSTDIGRHIRKVTAGSGKKLSLELGGKSPFIV
                     FDDADLDSVVEGVVDAIWFNQGQVCCAGSRLLAQEGIAARLEEKLRYRMERLRIGDPL
                     DKAMDIGAIVGPVQLQQIQKLVQQGVDEGAYMWQPSWSCPTEGSFFPPTLFTNVSPSS
                     TIAQTEIFGPVLVSMTFRTPAEAVALGNNTRYGLAASVWSENINLALDVAAKLKAGTV
                     WINSTNQFDAASGFGGYRESGYGREGGKEGLYEYVKPAWEAREPSAEYRVDGKAGAAS
                     NGHTASNGHADGNGHHELPEDELELLGIQHSVLGTPAIDRTPKLFIGGKQARPDSGYS
                     MAVYGPDGELIGEVGQGNRKDVRNAVEAAHAAEGWASTTGHNKAQILY"
BASE COUNT          299 a          678 c          617 g          392 t
ORIGIN      
        1 gtagagtatc tgcgccttgt tgtgccccgt ggtagaggcc cagccctcgg cggcgtgcgc
       61 ggcttccacg gcgttgcgca cgtccttgcg gttgccctgc cccacctcgc ctattagctc
      121 tccgtcgggg ccgtacaccg ccatgctgta gccggagtcg ggccgcgcct gcttgccgcc
      181 aatgaacagc ttgggcgtgc ggtctatcgc gggagtgccg agtaccgagt gctgaatgcc
      241 gagtaattcg agctcgtcct caggcaactc gtggtgaccg ttgccgtcag cgtggccgtt
      301 gctcgctgta tgtccattac ttgcggcccc tgccttccca tccacccggt actcggcgct
      361 cggttctctg gcctcccagg cgggcttgac gtactcgtac agcccctctt tgccgccttc
      421 gcgcccgtag ccgctctccc tgtagccgcc gaagccggag gccgcgtcga actggttggt
      481 gctgttgatc cagaccgtgc ccgccttgag cttggcggcg acatccagcg ccaggttgat
      541 gttctcgctc cacaccgagg ccgcaaggcc gtagcgggtg ttgttgccca gggccaccgc
      601 ttcggcgggc gtgcggaagg tcatcgacac cagcacgggg ccgaatatct ccgtctgggc
      661 gatggtcgag gagggcgaca cgttggtgaa gagggtcggc gggaagaagc tgccctcggt
      721 ggggcacgac cacgacggct gccacatata cgcgccctca tcgaccccct gctgtaccag
      781 cttctgtatc tgctgcaact gcaccgggcc gactatggcc cctatgtcca tcgccttgtc
      841 gagcgggtcg cctatgcgca gccgctccat cctgtagcgc agcttctctt ccaggcgcgc
      901 cgctatgccc tcctgggcca gcaggcgcga cccggcgcag cacacctgcc cctggttgaa
      961 ccagatggcg tccaccacgc cctccaccac gctatccagg tcggcgtcat cgaacacgat
     1021 gaagggcgac ttgccgccta gctccagcga cagcttcttg ccactgcccg ccgtaacttt
     1081 gcggatatgt cgcccgatgt cagtggagcc ggtgaaggcg atcttgtcca cgccggggtg
     1141 ggtcaggatg gcctcgcccg ccgtccgcgc ctcacccgtc aggatgttca ccacgccctt
     1201 agggaggccc gcctcgtcgc aaatgtgggc gaaggccagc gcggtgaggg gcgtgtagct
     1261 ggcgggtttg agcaccaccg tgttgcccat cgctatagca ggcgcgatct tccaggccat
     1321 catcagcagc gggaagttcc aggggattat ctgccccacc acgcccacgg gcaccatacc
     1381 gggcaactcc gtctccatca gttgcgccca gcccgcgtga tagtagaagt ggcgggcaac
     1441 cagggggatg tcgatgtcgc gggtctcgcg tatcgtcttg ccgttgtcaa ggctttcgag
     1501 cacggccagc aggcgggagt gcttctgcaa caggcgggca atggcgtaca tgtagcgggc
     1561 gcgtacgtgg ccgggcgtgg cgccccagct ctcgaaggcg cggcgtgcgg cggctatcgc
     1621 tctatccgcg tcctcggccg taccgagggc caccttcgcg agggacttgc cggtcgcagg
     1681 gtcctggctg tcgaagtact tgccgctacc cggcacgacc cactcgccgt cgatgaagag
     1741 gccgaacggc tggtgccgct cgatccactg caaagcaggc tccttggcct caggggccgg
     1801 gccgtaatcc aacgtctcga agatttccgc cactgtagcc atgtgagtcc ctcccgctgc
     1861 taccggcctc caactgccgg tcctcgtctt gtctatgcgt acatcgtacc acgttttatg
     1921 cttcggataa agccccaccc cgtcagcctg tatccctctt ttgtcatcct gagcgtagcg
     1981 aaggat
//
LOCUS       DASRWZ010000586         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_321824, whole genome shotgun sequence.
ACCESSION   DASRWZ010000586 DASRWZ010000000
VERSION     DASRWZ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321824"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1471
                     /locus_tag="VFH60_09200"
     CDS             113..1471
                     /locus_tag="VFH60_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET6314000.1"
                     /translation="MRALLASLGRGAGWKLMSDLLGRVLQYALLMLAARSLDLAGFGD
                     FTFALSVGLMLSQVADFGLQLYLQRELARLAIPEATQRPYFIDERATARLVGGGLVIK
                     VGLSAVSFAVMLLVVLWEPVGNKGPVLLIGLSMIGLTALEYLAYCFRALRRLKNEAIA
                     YVTGRAINLLLGAALLYAGLDVWALAVAANVAAVVAIVYSYSRLLAYVRPDYTLDLAY
                     WRRSLGQPVAMGLGIVFSMFAFRVDNLLIPPIVGAAQASETLSIYNAAYRLFEMTLIV
                     PGVVLAATFSLLSRAGGSGGSGSGGGVGGLRPVLGQTMMVLLGLGVLASIALWVGAVP
                     LTGLLYGDKFGAAVPVLQVLALGCIPMYASYGLTHALVAVDRPQLYAAFTGVALVVNV
                     AANLLLIPRVGVQGAAFATFLTEVALVVLCAAAVLRHMRGSSMAMPAQVVQSETGVGG
                     TL"
     gene            1468..>2140
                     /locus_tag="VFH60_09205"
     CDS             1468..>2140
                     /locus_tag="VFH60_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314001.1"
                     /translation="MSATAKGGVTATSIPAPANVPTVGVASRLDVLTDRLLFAMLLLV
                     LLASPFEIGYPPLGRVLFATFTNLEVMLFCLAGALLFRLAVSAESRGSLLRMPLLLPA
                     LSLILASGVSTAFGEYKSLGVQFIYRLAMGLMVFASVWLALRGKRRVLVSLGTFVGAA
                     LISASIGLMEFLPGVNLEGWLMPFRPQPTTVGGLLRLSGSFEYANGAAVYFEMAFPVL
                     LGLAVL"
BASE COUNT          283 a          643 c          754 g          460 t
ORIGIN      
        1 ccctaagtca agtagctact gaactctgat caccacattc agcaactgag ttgccccaca
       61 gcacgtgtca cgcccaatcc tgtaactcac ctcgtaaagg gactctgtga ctgtgagagc
      121 gttgcttgct tcgttaggtc gcggtgcagg gtggaaactc atgtccgacc tgctggggag
      181 ggtgttgcag tatgccctcc tgatgctggc tgcccgcagt ctcgacctgg cggggtttgg
      241 tgacttcacc ttcgcgctca gcgtgggcct catgctctcg caggtggccg atttcgggct
      301 gcaactctac ctccaacgcg agctggcccg cctcgccata cccgaggcta cacagcggcc
      361 ctacttcata gatgagcggg cgacggcacg tctcgtgggt gggggcctcg ttatcaaggt
      421 ggggctatcg gcggtgtcgt tcgcggtgat gctgctggtg gtgctgtggg agccggtggg
      481 caacaaagga ccggtgctgc tgattggcct gtcgatgata gggcttaccg cgttggagta
      541 cctggcctac tgcttccggg cgttgcgcag gctcaagaac gaggcgatcg cgtacgtcac
      601 ggggcgcgcc ataaacctgc tcctgggggc ggccctgctg tatgcggggc tggacgtgtg
      661 ggcgctggct gtcgcggcca acgtcgcggc ggtcgtggct atcgtctaca gctacagtcg
      721 cctgctggcc tacgtgcgcc ccgactacac tttagacctg gcctactggc ggcgctcgct
      781 tgggcagccc gtcgcgatgg gcctgggcat cgtgttcagc atgttcgctt tcagggtgga
      841 caacctgctc atcccgccaa tcgtgggggc cgctcaagcc agtgagaccc tcagtatcta
      901 caacgcggcc tacaggcttt tcgagatgac cctcatcgtg ccgggcgtgg tgctggccgc
      961 gaccttttcc ctgctgtcaa gggcgggcgg ctccggtggc agcggtagcg gcgggggcgt
     1021 gggagggttg cggccggtgc tcggccagac gatgatggtg ctgctggggc tgggggtgct
     1081 ggcctctatc gcgctgtggg tgggggcggt gcctctgacc ggcctgctct acggcgacaa
     1141 gttcggcgcg gcggtgcctg tgctgcaagt cctggccctc ggctgcatac cgatgtacgc
     1201 gagctacggc ctgacccatg cgctggtggc ggtggaccgg ccccagttgt acgcggcctt
     1261 tacgggggtg gcgctggttg tgaacgtggc ggccaacctg ttgctcatac cccgcgtggg
     1321 ggtgcagggc gcggctttcg ctacctttct gacggaggtt gcgctggtgg tgttgtgcgc
     1381 ggcggccgtg ttgaggcaca tgcgcgggtc gagcatggct atgccggcac aggtggtaca
     1441 gagcgagacc ggcgtaggag gtacactgtg agcgcgacgg ccaagggagg cgtaactgct
     1501 acttctatcc ccgcacccgc gaacgtgcca acggtgggcg tggcttccag gctggacgtg
     1561 ctcaccgacc ggctgctctt cgccatgttg ttgctcgtcc tgctggcaag cccctttgag
     1621 atcggctacc ctcctctcgg cagggtgctg ttcgccacat tcaccaacct ggaagtaatg
     1681 ctgttctgcc tggccggggc cttgctcttc aggctggccg tgtcggccga gtcgcgcggg
     1741 agcttgctgc ggatgccgct gctcctgcct gcgctctcgc tcatcctggc ctcaggggtc
     1801 tccaccgcct ttggcgagta caagtcgctg ggggtgcagt tcatctaccg gctggcgatg
     1861 gggctgatgg tgttcgcctc ggtgtggctg gcgctgcgag gcaagcggcg ggtgctagtg
     1921 tcgctgggca cgttcgtggg ggcggccctc atctcggcgt ccatcggctt gatggagttc
     1981 ctgcctgggg tcaacctgga aggctggctc atgccgttca ggccgcaacc caccacggta
     2041 gggggcctgc tccggttgag cggcagcttc gagtacgcga atggggccgc cgtgtacttc
     2101 gagatggcct tccccgtgct gctcggcctg gcggtgctct
//
LOCUS       DASRWZ010000587         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_459104, whole genome shotgun sequence.
ACCESSION   DASRWZ010000587 DASRWZ010000000
VERSION     DASRWZ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459104"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3838
                     /locus_tag="VFH60_09210"
     CDS             <1..>3838
                     /locus_tag="VFH60_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET6314002.1"
                     /translation="PETAKYDLSLSMVDGEGGLACRLEYSTDLFDDTTIARMLGHFQA
                     LLESIAADPDCSISQLSMLAQEERQQLLVQWNDTESATPIPWQCIHELVERQVNRTPG
                     AVAASFKGEEWTYQQLNSRANRLAHHLRELGVGPEVPVGVCMERSLHMIAALLGVLKA
                     GGAYVPLDPAYPQDRLAFMLADSQAPVLLTQQALAGGLPEHGATVVYLDDWWEEDAGE
                     NESNVASGVKPDNLAYILYTSGSTGRPKGVSIEHKSAIAFIEWATQLYTPEELSRVLA
                     STSISFDLSVFELFVTLSCGGRVVLVENALHLPSLAPGEYVTLVNTVPSAIAELLRTV
                     GVPASVRVVNLAGEPLQQTLVQQVYEKVPGCRVFNLYGPTEDTTYSTFALMEKGSARL
                     PHIGRPISNSQVYLLDRHMQPVPVGVPGELYLGGAGLARGYLKRPDLTAERFVPTPVV
                     RSEGRGARGESIPHSAFRTPHSGGERLYKTGDLAAYLPDGNIQFLGRLDHQVKLRGFR
                     IELGEIEAVLRQHPAVRDTVLMVREDVAGDKRLVAYVVPAQAITPADLRGYLRKHVPE
                     YMVPSAFVLLEKLPLTPNGKLDRRSLPAPELERGEGREYVAPRSPVEEALVGIWASVL
                     GVERVGVHDNFFELGGHSLLATQALSRVRDVLHVEMTLRRIFETPTVAKLGESIEAAL
                     KGGSAAPAPPVQRVQHTGDLPLSFAQQRLWFLDQWMPASAAYNIPVVMRLNGPLHEDA
                     LKASINAIVGRHEALRTSFTVVGDRPAQVIAPTLDVPVPLADLSGLPGEARQVEAQRR
                     VKEEAQRPFDLARGPLLRANLLRLGEREHLLVLVMHHIVSDGWSMSVLTEELAELYRA
                     FAAGEEPSLPELPVQYADYALWQRGWLQGDALQAQLDYWKGQLAGAPSAIELPTDRPR
                     SPMLSYRGAREVQLLPNDLASDLKAFSQREGVTFYMTLLAAFGALLHRYTRQIDILVG
                     SPIANRTRPEIEGLIGFFVNTLVLRTDLSGDPSFRELLHRVREVALGAYAHQDLPFDR
                     LVEELHPERNQGIHPIFQVAFVLQNAPVVPLQLRDLSLTSIEIDKVDMGASRFDLMFI
                     VEETQEGLSVEVEYNTDLFDVTTISRMLGHYRTLLEGALAGSEQRVSKLPLLAEAERR
                     QLLVEWNDTRTEYPRDECVHKLFERQAQQTPDAIALVFGEENLTYGELNERANQLARY
                     LREMGVGPETLVVVCMDRSVEMVVALLGVLKAGGAYVPLDPTYPQERLRFMVHDTQIL
                     LLLTQSSLAEKLLAYGV"
BASE COUNT          709 a         1122 c         1279 g          728 t
ORIGIN      
        1 ccccgaaacc gcgaagtacg acctctccct gtctatggta gatggtgagg gtggactggc
       61 gtgccgcttg gagtacagca ccgacctgtt cgatgacaca accatcgctc gaatgctggg
      121 acatttccaa gccttgctgg agagcatcgc cgccgaccct gactgctcca tttcacaact
      181 atccatgttg gcgcaagagg agcggcagca gttattggtt cagtggaacg acaccgagag
      241 cgcaacaccc atcccctggc agtgcataca cgaactcgta gagcggcagg taaataggac
      301 gcccggcgcg gtggcggcca gcttcaaggg cgaagagtgg acttaccaac agctaaacag
      361 ccgggctaac aggctggcgc atcacctgcg ggagcttggg gtcgggccgg aggtgccggt
      421 gggcgtttgc atggaacgct ctctgcacat gatagccgcg ctgctggggg tgctcaaagc
      481 gggcggggca tacgtgcctc tggacccggc ttacccgcaa gaccgcctgg cctttatgct
      541 cgccgactcc caggcccctg tattgctgac gcagcaggcg ctggccggag gactgcctga
      601 gcacggggct acggtggtgt atctagacga ttggtgggag gaagatgccg gggagaatga
      661 gagcaatgtt gcgagtggag taaagcccga caacctggct tatatcctct atacctccgg
      721 ctccaccgga cgcccgaagg gtgtgagtat cgagcataag agcgcgattg cattcataga
      781 gtgggcaacg cagctttaca cgccggaaga gctttccagg gtgctggcct cgacttcgat
      841 ctccttcgac ctgtcggtgt tcgagttgtt tgtgacgttg agttgtgggg gcagggtcgt
      901 gctggtggag aacgcgctgc acctgccttc tctcgcgccg ggcgagtacg tcaccctggt
      961 aaacaccgtg ccgtccgcga ttgccgagct actgcgcacg gttggcgtgc ccgcgtcggt
     1021 gcgcgtggtc aacctcgccg gagaaccgtt gcagcaaacg ctcgtgcagc aggtatacga
     1081 gaaggtgcct gggtgccggg tgttcaacct gtatggcccg acggaggaca ccacctactc
     1141 taccttcgcg ctcatggaga aggggagcgc gcgattgccg catatcgggc ggcccatctc
     1201 caacagccag gtctatttgc tggaccgcca tatgcagccg gtgccggtgg gcgtgccggg
     1261 cgagctatac ctgggcggcg cggggctggc aaggggctac ttgaagcgcc ccgacctcac
     1321 agccgagcgg ttcgtgccca cccccgtggt gaggagcgag gggcgagggg cgaggggcga
     1381 gtcgattccg cattccgcat ttcgcactcc gcattcgggt ggggagaggc tgtataagac
     1441 gggcgacctg gcggcttact tgccggatgg taatatccag ttcctcggac ggctggacca
     1501 ccaggtaaag ctccggggct tccgtatcga gttgggggag atcgaggcgg tgctgaggca
     1561 gcatcctgcc gtccgggata ccgtgcttat ggtgcgggag gacgttgcgg gcgacaagcg
     1621 gttggtggct tacgtggtgc ctgcccaggc tatcacccct gctgatctgc gcggctatct
     1681 caggaagcat gtgccggagt acatggtgcc ttcggccttc gttttgttag agaagctgcc
     1741 gctcacgccc aacggcaagc tagaccggag atctctgccc gcgcccgaac tggagagggg
     1801 agagggcagg gagtacgtcg cgcctcgtag ccccgtagag gaggcgttgg tgggtatatg
     1861 ggcctcggtg cttggcgtcg agcgggtcgg ggtgcatgac aacttcttcg agttgggcgg
     1921 gcattcgctt ctcgcgacgc aggcgctatc cagggtgcgc gacgtgctgc acgtggagat
     1981 gaccctgcgc cgcatcttcg agacgcctac cgtagccaag ctgggcgaga gcatagaagc
     2041 agccttgaag gggggttccg ccgcgcctgc tccacccgtc cagcgcgtgc agcacaccgg
     2101 ggacttgccc ctctccttcg cgcagcagag gctgtggttt ctcgaccagt ggatgcccgc
     2161 cagcgccgcc tacaacatcc ccgtcgtgat gcgccttaac gggcctctgc atgaggacgc
     2221 gctcaaggcg agcatcaacg ccatcgtcgg gcggcacgaa gctctgcgga ccagcttcac
     2281 ggtcgtgggg gatcgcccgg cacaggtgat tgcccctacc ctggacgtgc cggtgcccct
     2341 ggcggacctg agcgggctac ccggcgaggc caggcaagtg gaggcccaac ggcgggtcaa
     2401 agaagaagcg caacgtccct tcgacctggc gcgcggccca ttgctgcgag ctaacttgct
     2461 gcgcctgggc gagagggagc acctgctggt gctggtgatg caccacatcg tgtcggacgg
     2521 ctggtctatg agcgtattga ccgaggagtt ggccgagttg tacagggcgt tcgccgctgg
     2581 ggaagagccg tccctacctg aactgcccgt gcagtacgcc gactacgcgc tctggcagcg
     2641 agggtggctg caaggcgatg cgctacaggc ccagcttgat tactggaagg gccaactggc
     2701 gggcgcgcct tcggccatcg aactgcctac ggaccggcca cgctcaccca tgctcagcta
     2761 tcgcggggcc agggaggtac agcttctccc caacgacctg gcttcagatt tgaaagcgtt
     2821 cagccagcgc gaaggggtga cgttctacat gacgctgctg gcggcgttcg gggcgctgct
     2881 ccaccgctac acaaggcaga tcgacatctt ggttggctcg cccatcgcca accgcacccg
     2941 gcctgagatc gaggggctga tcggcttctt cgtgaatact ctcgtgttgc gcaccgacct
     3001 ttcgggcgac cccagcttcc gagagttgct tcatagggtt agggaagtgg cgcttggagc
     3061 atacgctcac caggacttac ctttcgacag gctggtggag gagctacacc ctgagcgcaa
     3121 ccagggcatc caccctatat tccaggtggc cttcgtgcta cagaatgcgc ccgtcgtgcc
     3181 cctgcaactg cgcgacctga gcctgacgag catcgaaatt gacaaggttg acatgggcgc
     3241 gtcacggttt gacctgatgt tcatcgtgga ggagacgcaa gagggacttt cggtagaggt
     3301 ggagtacaac accgacctgt tcgatgtgac aactatcagc cgcatgctgg ggcactaccg
     3361 taccttgttg gaaggcgctc ttgcggggtc tgagcagcgc gtgtcgaagc tgccgctgct
     3421 tgcggaggcc gagcggcgac aactgttggt ggagtggaat gacactcgca cggagtatcc
     3481 gcgcgatgag tgcgtacaca aactcttcga gcggcaggcc caacaaacac ccgacgccat
     3541 cgcgctggtc tttggagaag aaaaccttac ttacggagag cttaacgagc gtgcaaacca
     3601 attagcgcgc taccttagag agatgggagt ggggccggag acccttgtgg tcgtgtgcat
     3661 ggaccgctct gtggagatgg tggtcgcgct gctgggcgtg ctgaaagcgg gtggagcata
     3721 cgtgcccctc gaccccacct acccgcaaga gcgccttcgc ttcatggtac atgacaccca
     3781 gatattgctt ctgttgaccc aatcctccct ggcggaaaag ctgttggcgt atggggtg
//
LOCUS       DASRWZ010000588         4195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1144531, whole genome shotgun sequence.
ACCESSION   DASRWZ010000588 DASRWZ010000000
VERSION     DASRWZ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144531"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFH60_09215"
     CDS             <1..588
                     /locus_tag="VFH60_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET6314003.1"
                     /translation="NLGWKLAQVVKKTSPDSLLDTYHTERHPVAARVLRDTMAQVALL
                     RTDDRTNALRDIASELLSMDEPRKRLAGMMSGLDLHYDLGEGHPLLGRRMPDLDVITD
                     EGPLRVFTLLHDARPVLLNLGKPGGFDITPWAHRVMSIEATYGGNWELPVLGQVAAPT
                     AVLIRPDGYVAWVGEDRDGADTGLRDALTTWFGSP"
     gene            complement(655..1635)
                     /locus_tag="VFH60_09220"
     CDS             complement(655..1635)
                     /locus_tag="VFH60_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET6314004.1"
                     /translation="MTPLTNDNAQLTPRTTFDNPKLVFDFPSLRIGVAEYAEGPTGCT
                     VFHFPQLAQAAVDVRGGSHGTLLTEERDRVGAICLAGGSLLGIEAATGVTAELFAQRN
                     YSTQWGDIPLVSAGIIFDYGPRGNSVYPDKALGRAALRAAESGVFPLGACGAGASATV
                     GKGLGFEMRERGGQGGSFRQVGQTRVAVFTVVNAVGAIVDRQGNVVRGHLDPATGQRY
                     HYRDGLAKKLAEAEPLEPTREGNTTLTVLVTNQKLNMERGLTWSLRQLARTVHSSMSQ
                     AIQPFHTLYDGDVLWAVTTNEVENPDLNDVALGVVAAEVAWDAVLSCFSE"
     gene            complement(1685..2578)
                     /locus_tag="VFH60_09225"
     CDS             complement(1685..2578)
                     /locus_tag="VFH60_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6314005.1"
                     /translation="MTRVAVLADIHGNLPALEAVARDLGQFRVDHVVVAGDLVNWGPY
                     SEQVLEFVTKRGWSVIRGNHEFYLLDYGTPRAPTEWQDSTQYAMLPWLRKQLAGKWQT
                     TIAAWPDALTLRFPDAPPVRVVHGSTRSHWEGIYPHSSYGDVKDALQVEEPVLVAAHT
                     HLPMDRVLGDRHVMNPGSVGVPLDGIFGASYLVMAGTPGGWQATFRRVPFEYEPLFRE
                     FERVRFVEECGVVGELVVAEFRTARPQVHTFMGWRKRHHPGAPLSVQLLAEFYKVDPD
                     EYNLPAYRLESIRSSLVQTGG"
     gene            complement(2652..3605)
                     /locus_tag="VFH60_09230"
     CDS             complement(2652..3605)
                     /locus_tag="VFH60_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET6314006.1"
                     /translation="MPERQQFTIMTVHAHPDDEVVFTGGTLALYRQRGARTVLVTATS
                     GEEGEIHDPDLDADEARPRLGEIRMEELRKAVDLLEISDFEPLGYRDSGMDGTEPNNH
                     PDSFNMADREQATARLVALVREYKPHVLITYDDFGAYGHPDHKACNVITLAAFDKAAD
                     PSYRPDLGEPWQPLKLYYCNWAEENWKRAREMYIERGLKWPWDREEQAQQKDEDPEHQ
                     STEEGVIEQQVEQEKAEEEGKPPKYVPPPITTRIDVTGVFPQKRAATLVHRTQFDPDG
                     LFTNMPEDIALVAFGEEVYSLVRSRVDAPEQEDDILAGLSS"
     gene            3763..>4195
                     /locus_tag="VFH60_09235"
     CDS             3763..>4195
                     /locus_tag="VFH60_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6314007.1"
                     /translation="MAQPTSTIQADFDRLARLTAAQGWSHNDHYHGYLLRHVPGRCRE
                     ALDIGSGTGAFSRCLATRADNVLGLDLSPEMVRVATSMSRGYTNLEYRVADVMEIDLP
                     DEHYDGRASIATLHHMPMEAILRKMTAALARNGTLLVLDLYE"
BASE COUNT          722 a         1328 c         1329 g          816 t
ORIGIN      
        1 aacctggggt ggaagctggc ccaggtggtc aagaagacat cgccggacag ccttctcgac
       61 acgtaccata ctgagcggca cccggttgct gcccgcgtgc tgcgcgacac gatggcgcaa
      121 gtcgcgctgc tccgcacaga cgaccgcacc aacgctttac gcgatatcgc ttccgagctg
      181 ctgagcatgg acgagcctcg caagcggtta gcagggatga tgtccggtct ggaccttcac
      241 tacgacctcg gtgagggaca cccgctgctc ggccgccgca tgcccgatct cgacgtgatc
      301 accgacgaag gcccgctgcg ggtatttacc ctgctgcatg atgcccggcc cgtgctactc
      361 aacctcggta agcccggcgg cttcgacatc actccatggg cacaccgagt catgtcgatc
      421 gaggccacat atggcggcaa ttgggagctg ccggtcctgg gccaggtcgc ggcccccact
      481 gccgtgctga tcagacctga tggatacgtc gcctgggtgg gggaggatcg ggacggcgca
      541 gataccggtt tgcgcgacgc gctgactacc tggttcgggt caccatgacg cggtacgatg
      601 ctccttggcg tggtcccggt cgtacttggc gtcctggcgg ttccgtctct tgctctattc
      661 cgagaagcag ctaagcaccg cgtcccaggc tacctcggcg gcgaccaccc ccagggctac
      721 gtcgttcagg tcggggttct cgacctcgtt ggtggtgacg gcccacagca cgtcgccgtc
      781 gtacagggtg tggaagggct gtatggcctg gctcatggag ctatgcaccg tgcgggcaag
      841 ctggcgtagc gaccatgtaa gcccgcgttc catgttgagc ttctggttgg tgacgagcac
      901 tgtgagggta gtgttgccct cgcgtgtagg ctcgagcggc tcggcctctg ccagcttctt
      961 cgccaggccg tcgcggtagt ggtagcgctg acccgtggcg gggtcgagat gcccgcgcac
     1021 gacgttcccc tggcggtcca cgatagcccc caccgcgttg accaccgtga acacggccac
     1081 cctggtttgc cccacctgcc tgaacgagcc gccctggccg cccctctccc gcatctcgaa
     1141 ccccaacccc ttgcctaccg tggccgaagc gcctgccccg cacgcgccga gaggaaagac
     1201 cccgctctcg gccgcgcgca acgccgccct acccagcgcc ttatcggggt agaccgagtt
     1261 gccccttggg ccgtagtcga agatgatgcc cgccgacacg aggggtatgt cgccccattg
     1321 cgtcgagtag ttgcgctggg cgaacagctc ggcggtgacg ccggtggccg cctcgatgcc
     1381 caggagcgag ccgcccgcca ggcagatagc gcccacgcgg tcgcgctcct cggtgagcag
     1441 ggtaccgtgc gagccgccac gcacgtccac cgccgcctgg gccagttggg ggaagtggaa
     1501 caccgtgcag ccggtcggcc cctccgcgta ctcggccaca cctattcgta gcgacgggaa
     1561 gtcgaatacc agcttcgggt tgtcgaaggt ggtgcgaggg gtcaattggg cgttatcgtt
     1621 cgttagcggg gtcatacata gggccttttg ggtgtttgga gttgtagtgg tttcgatagg
     1681 acagctagcc gccggtctgg actaaagacg agcgaatgct ctccagccgg tacgccggta
     1741 aattatattc gtccgggtcc accttataga actcagccag taactggacc gataggggtg
     1801 cgccggggtg atggcgctta cgccatccca taaaagtgtg tacctggggt ctggcggtgc
     1861 ggaactcagc caccactagc tcaccaacca ccccgcactc ttccacgaaa cgcacgcgct
     1921 cgaactctct gaagagcggc tcatactcga aggggacgcg ccggaaggta gcttgccagc
     1981 ctcccggagt gccagccata accaggtagc tagcgccgaa tatcccgtcc aacggcacgc
     2041 caacggaacc ggggttcatg acgtgcctat cacctagtac cctgtccata ggcaggtgag
     2101 tatgcgccgc gacaaggacc ggctcctcaa cctggagggc gtctttcaca tctccatacg
     2161 aggaatgagg atatatgccc tcccagtggc tcctggttga gccatggacc acccgtacgg
     2221 gcggcgcgtc cggaaaccgc agcgtcagtg cgtcgggcca tgcagcaatt gtcgtctgcc
     2281 atttgccggc caactgcttt cgcaaccagg ggagcatggc gtactgggta ctgtcttgcc
     2341 actcagtggg cgctctgggg gtgccgtagt cgagtagata gaactcgtgg ttgcctcgga
     2401 taacggacca gccccttttg gtaacaaact ccaacacctg ctccgagtac ggaccccagt
     2461 tgacgaggtc ccccgctacc actacgtggt caacccggaa ttgccccaaa tctcgcgcca
     2521 ctgcctcaag cgcgggcagg ttgccgtgga tgtcggccag tacagcgact cgggtcataa
     2581 gaggttgggg gcctttcgct tctcgcggct aaccgggcgg tccagaactt agcagacaac
     2641 tgcccgtcag gtcaggagga aagcccggca aggatgtcgt cttcctgctc cggggcgtcc
     2701 acgcggctgc gcaccaggct gtagacctcc tcgccgaacg ccaccaacgc gatgtcctcc
     2761 ggcatattcg tgaataagcc atcgggatcg aactgggtgc ggtggaccag ggtggccgcg
     2821 cgcttctgcg ggaatacacc cgttacgtcg atgcgggtgg tgataggagg gggcacgtat
     2881 ttgggcggct tgccttcttc ctcggccttc tcctgctcca cctgctgctc tatcacgcct
     2941 tcctcggtgg actggtgctc cgggtcttcg tccttttgtt gggcttgctc ctcccggtcc
     3001 cagggccact tcaggccgcg ctcgatgtac atctccctgg cgcgcttcca gttctcctcg
     3061 gcccagttgc agtaatacag cttgaggggc tgccacggct cgcccaggtc gggccggtac
     3121 gatgggtcgg ctgctttgtc gaaggcggcg agggtgatga cgttgcaggc cttgtggtcg
     3181 gggtggccgt aagccccaaa atcgtcgtag gtgatgagca cgtggggctt gtactcgcgc
     3241 acgagggcca ccaggcgggc cgtggcctgc tcgcggtcgg ccatgttgaa gctgtcgggg
     3301 tggttgttgg gttcggtgcc gtccatgccg gagtcgcgat agccaagagg ctcgaagtcg
     3361 ctgatttcca gcagatcgac cgctttgcgc agttcctcca tgcgtatctc acccaggcgg
     3421 gggcgggctt cgtctgcgtc gaggtcgggg tcgtgtatct cgccctcctc gccgctcgtg
     3481 gctgtgacga gcaccgtcct tgcgcctcgc tgccggtaga gggccagcgt gccgccggta
     3541 aagaccactt catcgtcggg gtgcgcatgg accgtcatga ttgtgaattg ctgtcgttcg
     3601 ggcactggta tattctcctc cgcaattgat tcgttgaagt gtgactctta tcatgcatgg
     3661 tgctattatg tcaatgtaga ttctatgagg aagccacatt aatcaagaag ctgccgcgta
     3721 acttctgaca tgagattata atggtctacg agaggtgcag caatggcaca acccacatcc
     3781 acaatccagg ccgacttcga ccgcctggcc cgtctcacgg ccgcgcaggg gtggagccac
     3841 aacgaccact accacggcta tctactgagg cacgtcccag gccgctgccg ggaggcgctg
     3901 gacatcggca gcgggacggg ggcgttctcg cgctgcctgg cgacacgggc ggacaacgtg
     3961 ctcggcctcg acctgtcgcc ggagatggtg cgcgtcgcca cgtccatgtc caggggatac
     4021 acgaacctgg agtaccgggt cgcggacgtg atggagatcg acctgccgga cgagcattac
     4081 gacggcagag cctccatcgc cacgctgcac cacatgccga tggaggccat actgcgcaag
     4141 atgacggcgg ccctcgcacg caatggcacc ttgctggtgc tggacctcta cgagg
//
LOCUS       DASRWZ010000589         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_185205, whole genome shotgun sequence.
ACCESSION   DASRWZ010000589 DASRWZ010000000
VERSION     DASRWZ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185205"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VFH60_09240"
     CDS             <1..1020
                     /locus_tag="VFH60_09240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET6314008.1"
                     /translation="GPPSTALLLLPLTFLPFAASITVYRVLVVLAFAASVYVAGLALP
                     TNGRLTGWSVGGLALLCFQPVVLSLGLGQVDAFVMLALAVGIWAAGRERWWLAGAAIG
                     FAALLKVSPGLLLLYLLLRGKYRAVLGAAITVVALLALAALAGRPGDLWLFLSEVAPS
                     LSAGSLHVQNQSLPAWIARLSLPEADLLAYGRGLGPFSPLGLLLSGLATIALYLTRRK
                     LPLSPLDYGFLVLAALLAGPLTWDHYTSWAVLSLVLLSDPRSWRELTPRTRAGLLCLL
                     AAGALLMLGPGLAFSPQAVLANPALRLLTGTKTVALLLFCAVTLYLLVRASRSPTSQA
                     VARGG"
     gene            complement(1021..2553)
                     /locus_tag="VFH60_09245"
     CDS             complement(1021..2553)
                     /locus_tag="VFH60_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017572879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6314009.1"
                     /translation="MSTLHDVLESSGHPDFPGAQVRSDDPRYPALVRGFNLRWVGAPK
                     YVQVCGSAEQVRDAVQQTLDAGLSVTVRGGGHCYEDFVSNNDGGVIIDLSPLNAVYSP
                     GDGYYAVEGGCTLWNVYTQLFKEYGVTMPAGSCYSVGAGGHVVGGGYGLLSRKYGLVI
                     DYLYAVEVVTVTADRKAVLTLVTRDEDDPVRRELFWAHQGGGGGNFGIVTTYWFKDLP
                     KAPACAYLTTVAWEWADIGKAEFTELVTNYGTFFEQNSGVDSPYKDMFALLHLSNQAA
                     GQISLTVQNVGPDADLIDTFLEAISPGGVSRQALRVPVGYLHAAPPPEQTQYLPWLDA
                     TQTVNGTGPPRRGKYKSAYMNKGFPPEQIEVLWRYLWEEKFNNSQALVQIDSYGCQIN
                     AVPSDATAVPQRSSIMKLQYQTYWTDESDDQQNLDWINGFYNAMYGPEGPVPDGTMDG
                     CYVNYPDVDLTHWQLLYYGEQGYKRLQQVKARWDPLNVFNHKQSIELPGKVEGGAAKS
                     EG"
BASE COUNT          430 a          959 c          924 g          554 t
ORIGIN      
        1 ggcccgccct cgaccgcgct gctgctcctg cctttgacgt tcctgccctt cgccgcctcc
       61 atcacggtct accgcgtact ggtcgtgctg gctttcgcgg cctcggtgta cgtggcgggc
      121 ctggcgctcc ccaccaacgg caggctcacc ggctggagcg tcggggggct ggcgctgctc
      181 tgcttccagc cggtggtcct gtcgctgggg ctggggcagg tggacgcctt tgtgatgctg
      241 gcgctggcgg tgggaatctg ggcggcaggc cgggagcggt ggtggttggc gggcgcggcg
      301 ataggcttcg cggccctgct caaggtcagc cccggcctgc tgcttctcta cctgctgctc
      361 agaggtaagt accgggccgt gctgggggcc gccatcacgg tcgtcgccct cctcgcgcta
      421 gcagccctgg cggggaggcc cggcgacctc tggctctttc tcagcgaagt cgccccctct
      481 ctatccgcag gctcgctgca cgtgcaaaac cagtccctcc ccgcctggat agcgcgcctc
      541 tcgctgccgg aggccgacct gcttgcttac gggcgtggac tcggcccgtt cagtcccctg
      601 ggactcctcc tgagtggcct cgcgactatc gccctctacc tcacaaggcg caagctaccg
      661 ctctcccccc tcgactacgg cttcctggta ctggccgcgc tcctcgccgg gccgctcacc
      721 tgggaccact acacgagttg ggccgtgctc agcctcgtac tgctcagcga ccctcgctcg
      781 tggcgcgagc tgacaccccg aacgcgtgcc gggctgctct gcctgctcgc cgccggtgcc
      841 ctgctcatgc tggggccggg gttggcgttc tccccacagg cggtgctcgc caaccccgca
      901 ctcaggctgc tgacaggcac gaagaccgtg gccctgctcc tgttctgcgc ggttacgctg
      961 tacctgctgg tgcgtgcgag ccgttcgccc acctctcagg ctgtcgcgcg gggtggataa
     1021 tcagccctcg ctcttcgcgg ccccgccctc caccttgccg ggcagctcga tagactgctt
     1081 gtggttgaag acgttcagcg ggtcccagcg tgccttcacc tgctgcaacc gcttgtaccc
     1141 ctgctcgccg tagtacagca gttgccagtg cgtgaggtca acgtcggggt agttcacgta
     1201 gcagccgtcc atggtgccat cgggcaccgg cccctccggc ccgtacatgg cgttgtagaa
     1261 gccgttgatc cagtccaggt tctgctggtc gtccgactcg tctgtccagt acgtctggta
     1321 ttgcagcttc atgatcgagg agcgttgggg gaccgccgtg gcgtccgagg gcaccgcgtt
     1381 gatctggcag ccgtaagaat cgatttgcac cagggcctgt gagttgttga acttctcctc
     1441 ccacaggtag cgccacagca cctcgatctg ctcgggtggg aaccccttgt tcatataggc
     1501 cgacttgtac ttgccacggc gcgggggacc ggttccgttc acggtctggg tagcgtcgag
     1561 ccagggcagg tactgggtct gctcgggagg gggcgcggcg tgcaggtagc cgaccggcac
     1621 cctcagcgcc tgccgcgaca ccccgcccgg cgatatagcc tccaggaacg tgtcgatcag
     1681 gtcagcgtcg gggcctacgt tctgcacggt caggcttatc tgcccggcgg cctggttgga
     1741 gaggtgtagc agcgcgaaca tgtctttgta agggctgtcc acgccgctgt tctgctcgaa
     1801 gaacgtgccg taattggtca ccagctcggt gaactcggcc ttgcctatgt cggcccactc
     1861 ccaggctacg gtggtgagat aggcgcaggc gggcgctttc gggaggtctt tgaaccagta
     1921 cgtggtgacg ataccgaagt tgcccccgcc gcctccctgg tgcgcccaga aaagctcgcg
     1981 gcggaccggg tcatcctcgt cccgcgtcac gagcgtcagc accgccttgc ggtcggcggt
     2041 caccgtaacc acctcgactg cgtacaggta gtcgatgacg aggccgtact tgcgcgacag
     2101 caggccgtag ccccctccca cgacgtgccc gcccgcgccc acggaatagc aggagcctgc
     2161 gggcatggtg acgccatact ccttgaaaag ctgggtgtag acgttccaca gcgtgcagcc
     2221 gccctcgacg gcgtaatagc catcccctgg gctgtagacc gcgttgaggg gcgagaggtc
     2281 tataatcacg ccgccgtcgt tgttcgacac gaagtcctcg tagcagtgcc cgccgccgcg
     2341 caccgtcacg ctcagacccg catccagcgt ctgttgcacc gcgtcgcgca cctgttcggc
     2401 gctaccgcag acctgcacgt acttcggcgc gcccacccag cgcaggttga atccacgcac
     2461 gagggcgggg tagcgcgggt cgtcggaccg cacctgcgcc cccggaaaat cggggtggcc
     2521 ggaggactcc aggacatcgt gtagggtgct cataagtggc tcctttctga gtgaaaatgc
     2581 aagcggcccg ttgctcctaa gccaggcaaa cgcgagaaga cccacgcgta aggcaggcac
     2641 catacatagc gggcgcgcct agtatatcac cgctgaaggt gcttgtcaat tagcagtaga
     2701 ctatagcgag ggcatagtca ttcccggcag ggtggcttgg cgatgccgtg tggcttatga
     2761 agatcaacgg gttaatgagg taaacaccag gggtgaaagg tgtagatgta ggggcaggcc
     2821 cccgtgcctg ccctggtgca aggcgtacac aggtggagat taccacc
//
LOCUS       DASRWZ010000590         7244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1282164, whole genome shotgun sequence.
ACCESSION   DASRWZ010000590 DASRWZ010000000
VERSION     DASRWZ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282164"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFH60_09250"
     CDS             complement(<1..345)
                     /locus_tag="VFH60_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314010.1"
                     /translation="MNKGRIRFIYIFAVVVLVAMGLPSAPSTGYAQGTGSRTFPQTGK
                     TVTGRFLHYWDTNGGLDQQGYPVSEVLQERSEVNNRVYFVQYFERAVFEFHPENPFPS
                     DVLLSLLGVFRYQ"
     gene            complement(460..1188)
                     /locus_tag="VFH60_09255"
     CDS             complement(460..1188)
                     /locus_tag="VFH60_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HET6314011.1"
                     /translation="MLKLPTINGTIKRRVLVNFRADPEVVQRLLPAPFRPKLHEGQAV
                     VGICLIRLEHLRPRLMPEFAGFSSENAAHRIAVEWVDEQGVPREGVFIPRRDTNSPVN
                     LLLGGRLFPGEHHRARFKVREEEEAVAVSMRSADGEVAVSVKGRLADGMPDSSIFDSV
                     DDASRFFEAGYLGYSMASARDRLDALELHTDQWHVEPLAVSSVHSSFFADESLFPRGS
                     VRFDHALLMRDIEHEWHTAATLYL"
     gene            complement(1368..2417)
                     /locus_tag="VFH60_09260"
     CDS             complement(1368..2417)
                     /locus_tag="VFH60_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314012.1"
                     /translation="MFGDKKKWLALGLVAMLALTTLPAATPASAQGQRFFPETNKTVK
                     GRFLEYWNQNGGLSQQGYPLTEEMREVSDTNGQTYTVQYFERAVFEHHPENPRPYDVL
                     LQLLGSFLYNQKYRGNAPNQQPNTAAGSRLFTETGKRVGGRFLDYWNRNGGLAQQGLP
                     ISEEFNEVSDLNGQTYRVQYFERAVFEYHPENQPAFQVLLSQLGTFRLRAKQQGGGGG
                     GGNQPPAPNARATACGNLPNNVPSNDWAASDKLQVRGGETIQFAAGVFRPAEPMSFWF
                     TFPDGSVLGTGSPLRPEDVEGTNFEVIPNGEGILGPVPIRIPEVGREYPGIWAITFQG
                     SQSQRQAVIYFCVTP"
     gene            complement(2721..6089)
                     /locus_tag="VFH60_09265"
     CDS             complement(2721..6089)
                     /locus_tag="VFH60_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET6314013.1"
                     /translation="MPSRAACEHCGAALPQEHVESRLMQADMGSWPAQVIAQEEAPAV
                     EQEGLVAADAVVETGVAYVPKKRHTKRGDDYYFQLYFDYMDAAETHADGAGNTQANTV
                     PGNGTANGASNGANNGNGNGNGNGHHGDGAHGGQAVDLAIAETVPLTLFGESDMGNGV
                     PDSATVEAQDKATSAGNYNDFEYELINDPSRLAQVAEMLAHETVLGVDTETTGLDPHI
                     SELLLLQVSTADKVYIVDCRRLVPLALKSVLENPNVLKIAQNAKFEYEMLKQQCGITL
                     TNMYDTMLAERICTAGLGREISLKQIAARYVGAVLDKSVREAFYKLAHNGDAYLADEQ
                     LHYAARDAFIMIPIWRLQKQELKKHRLEDVADLEFRCIAAVGDLELAGVQIDTVKWRK
                     IIEDVALHRERTAVELSEMLQPATMQATMFGIPSINLNSSTQLMEVFSKLGVDLPDTS
                     EATLSKFNHPAVAKLLEYRSHEKTLSAFGENVLGLINPKTGRIHPDFNQHGADTGRFS
                     CTRPNVQQIPATSDFRKCFVASEGYKLITCDYSQAELRILAELSGDPGFVGAFQSGDD
                     LHSLTASQMFGVPLAQVQKSQRSAAKAIIFGLAYGMGPGGLAPRLGVTLDEAKDLISR
                     YFKAYPGVQQWLEKAGRDAVRNGYSVTMLGRKRFYNIPDESLKKINEDEWRKQIAAIE
                     RQGKNSPIQGCVGGDTRIFEETRGYVPIAGLRGQVVSVWDGSRFSQAVVVYSGKKQLV
                     KVHLWDGNYIECSPDHRFLVRPTSGNDLWRTPAEFKPQQRVVLGEAVPEWGTPVSIPA
                     HNCNGSWNAKDTSLGQISDPAALGEWLGRLASDGTVTSGTAALMVAEHEEVLLPRLQA
                     ISAELGHVGHSIRSTESKPKRFHKLTVSSRGLAQQLRELGVKERVPDCAWQDSRVLAG
                     YLRGMFDGDGTVNSDGAFLVFGQRDKHLAWAREIQQALLLFGVRSRINVCADRVNVRV
                     VKKDMPLFCHYIGFMNPAKQAKAAEVAASPRNKTYGRGVRVKRVEFTEEWVDMYDVVN
                     SETSRFMANGMVVHNCNADMTKLALIYLRTALKDWDARTVNTVHDEIVVEARADVAEE
                     VQHIVEDCMVRAGQYILRVVPIVAEASLADYWSK"
     gene            complement(6544..6906)
                     /locus_tag="VFH60_09270"
     CDS             complement(6544..6906)
                     /locus_tag="VFH60_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET6314014.1"
                     /translation="MSSILVHVTTGPENPTRAALAFLVAKSAIEEGHTVSLFLAGDAV
                     QLIRDAVLDNLVGLGTGKLRELYDAIVAGGGKFYLSGMSSKARGVSAADIEGKPCEMA
                     MPNVLVRLSLEHDRMFTY"
BASE COUNT         1316 a         2298 c         2155 g         1475 t
ORIGIN      
        1 ctggtagcga aacacgccaa gcaacgacag taacacgtcg ctaggaaagg ggttctccgg
       61 gtggaactcg aacaccgcgc gctcgaagta ttggacgaag tagacccggt tattcacctc
      121 gctccgctcc tgcaacacct cagacacagg gtagccttgc tggtccagcc ctccgttcgt
      181 gtcccagtag tgcaagaacc tgccagtaac ggtcttgccg gtctgcggga aggtgcgact
      241 gccggtgccc tgcgcgtagc cggtggacgg ggcggaaggc agccccatcg cgaccagcac
      301 taccaccgcg aagatataga tgaatcgaat cctacccttg ttcatacagt tgcccttcta
      361 acgcgccact acaaggcgca gccgaactta ccccctgcct cgtgggcgga gacagacaac
      421 ggtgttttac gtccgtgtcc ccactgccct gggacctgct tacaggtaca gggtagcagc
      481 agtatgccac tcgtgctcta tgtcacgcat aagcagcgcg tggtcgaagc gcacgctgcc
      541 gcgcgggaac aggctttcgt ccgcgaagaa gctggaatga acgctgctga ccgccagcgg
      601 ctccacgtgc cactggtccg tatgcaactc taacgcatcc agccggtcgc gtgcggaggc
      661 catcgagtag cccaggtagc ccgcctcgaa gaagcgcgag gcgtcgtcca ccgagtcgaa
      721 tatggaagag tcgggcatgc cgtccgccag ccggcccttc acgcttaccg ctacctcccc
      781 gtccgccgag cgcatggaaa cggccacggc ttcctcttcc tcccgcacct tgaacctggc
      841 cctgtgatgc tcgccgggga acaaacgccc gcccaacagc aggtttaccg gtgagttggt
      901 gtcgcggcgg ggtatgaaca cgccctcgcg cggaacgccc tgctcatcga cccactcgac
      961 cgctatgcga tgggccgcgt tttcgctact gaacccggcg aattcgggca tcagcctggg
     1021 acgaaggtgc tccagccgga tgaggcatat ccccactacg gcctgcccct cgtgtagctt
     1081 gggcctgaag ggtgcgggca gcagtcgctg caccacctcc gggtccgccc tgaaatttac
     1141 cagcacgcgc cgcttgatgg tgccattgat cgtgggtagt ttcagcatga tgtggctcct
     1201 acttccgcgc cctttgctta gtcattcggc aagtcacact tgcactatat caccaaatag
     1261 cattgccttg catgcccgaa attggcacgc gctagtggga caaataaaag agggagggga
     1321 acaactcccc accctctctt gcatgtagct atcggttgtc gcccaggcta gggggtaacg
     1381 cagaagtaga ttaccgcctg cctctggctc tgggaaccct ggaaggtgat agcccagatg
     1441 ccggggtact ccctgccgac ctccggtatc ctgataggca cggggccgag gataccctca
     1501 ccgttgggga ttacctcgaa gttggtgcct tccacgtcct ccggccggag ggggctgccg
     1561 gtgccgagca ccgagccatc agggaaggtg aaccagaacg acatcggctc ggcgggccgg
     1621 aacacaccgg cggcgaactg gatcgtctcg ccaccgcgca cctgtagctt gtcgctggcg
     1681 gcccaatcgt tgctgggcac gttattgggc aggttgccgc aagccgtggc ccgtgcgttg
     1741 ggcgcgggag gctggttgcc gccgccgcca ccgccaccct gctgcttggc gcgaagcctg
     1801 aaggtgccca actgcgacag gagcacctgg aacgcgggct ggttctcagg gtggtactca
     1861 aacaccgccc gctcgaagta ctgcacccgg taggtctggc cgttcaggtc gcttacctcg
     1921 ttaaactcct cagaaatagg caggccctgc tgggcgaggc cgccgttgcg gttccagtag
     1981 tcgaggaacc tgccgcccac gcgcttgccg gtctcggtga acaaccgcga gcctgcagcg
     2041 gtgttgggct gctggttggg ggcgttgccg cgatacttct ggttgtacag gaagctgccc
     2101 aacagttgca gcagcacgtc atacgggcgg gggttctccg ggtggtgctc gaacaccgcg
     2161 cgctcgaagt actgcaccgt gtaggtctgc ccgttcgtgt cggagacctc gcgcatctcc
     2221 tcagtcaggg gatagccctg ctgggagagg ccgccgttct ggttccaata ttccaggaac
     2281 cggcccttga ccgtcttgtt cgtttcgggg aaaaagcgct gcccctgtgc gcttgccggg
     2341 gtggccgccg gcagcgtggt gagcgccagc atggctacca ggccgagtgc gagccatttc
     2401 ttcttatcgc caaacattat atattgcctc ctcaaagcta gatcgttggg gtttgtccat
     2461 gattcttact tgccatatta tccggttgtt gcttgaacat gcaaggtcca ttctgtgtct
     2521 agagctacca ctttgcgctg ctacataggc gaggctcctg tcattctcgt gtatgggaag
     2581 gtcgtgggtg ccgcacgagt atttatgtgc agcacaattc ttctgactgc attatatagt
     2641 cttaagaggg aaattgggtg ttacaaaaat acggacaaat agtggtacag aacgaggcag
     2701 caagccccgg tcaagaacgc ctacttgctc cagtagtcgg caagcgaggc ttccgcgaca
     2761 atgggtacga ccctgaggat gtactgcccg gcgcgcacca tgcagtcctc gactatgtgc
     2821 tggacctcct cggccacgtc ggcccgggct tccaccacga tttcgtcgtg cacggtgttg
     2881 acggtgcggg cgtcccagtc cttgagcgcg gtgcgcaggt agatgagggc gagcttggtc
     2941 atgtcggcgt tgcaattatg cacgaccatg ccatttgcca tgaaccggct cgtctcgctg
     3001 ttcaccacgt cgtacatatc cacccattcc tcggtgaact cgacgcgctt cacgcgtact
     3061 ccgcgtccgt aggtcttgtt ccggggggaa gcggccacct ccgccgcttt ggcttgcttg
     3121 gcggggttca taaagccgat atagtggcag aacaacggca tatccttctt caccacgcgc
     3181 acgttcacgc ggtcggcgca gacgtttatg cggctgcgca ccccaaacag caacagcgcc
     3241 tgctgtatct ccctcgccca cgccaggtgc ttgtcccgct gcccgaacac caggaacgcc
     3301 ccgtcactat tcaccgtgcc gtcgccatcg aacataccgc gcaggtagcc cgccagcacc
     3361 cggctgtcct gccaggcgca atcgggcacg cgctccttga cgccaagttc gcgcaactgc
     3421 tgagccagcc ctcttgagga gacggtcagc ttgtggaatc gtttgggctt gctttcggtg
     3481 gagcggatag agtgtcccac gtgccctagc tcggcgctga tagcctgcaa cctggggagc
     3541 aacacttcct cgtgctcggc taccatgagc gcggccgtac cggatgtcac agtgccgtcc
     3601 gaggctagcc tccccagcca ctcgcccagc gcggccggat cgctgatctg gcccaggctc
     3661 gtatctttgg cgttccagga accgttgcag ttgtgtgccg gtatgctaac gggcgttccc
     3721 cactccggca ccgcctcgcc cagcaccacg cgctgttgcg gcttgaactc ggcgggggtc
     3781 cgccacaggt cgttgccgga cgtgggccgt accaggaacc tgtggtcggg actgcactcg
     3841 atgtagttgc cgtcccacag gtggaccttc accaactgct tcttgccgct gtacacgacc
     3901 accgcctgcg agaaccggct gccgtcccac accgagacca cctgcccccg caggcccgct
     3961 atgggcacgt agccccgcgt ctcctcgaat atgcgggtgt cgccgccgac gcacccctgg
     4021 ataggcgagt tcttaccctg tcgctctatc gcggctatct gcttgcgcca ctcgtcctcg
     4081 ttgattttct tcaggctttc atcaggaatg ttgtaaaaac gcttgcgtcc cagcatcgtg
     4141 accgagtaac cgttgcgcac cgcgtcgcgg cccgccttct ccaaccactg ctgcacgccg
     4201 gggtacgctt tgaagtagcg ggagatcagg tccttcgcct cgtccagcgt cacgcccagg
     4261 cgcggagcga ggccgcccgg acccataccg tacgccaggc cgaagatgat cgccttggcc
     4321 gccgaacgct gcgacttctg cacctgcgcc agcggcacac cgaacatctg cgaggcggta
     4381 agagagtgca agtcgtcgcc cgattggaac gcgcccacga agccggggtc acccgatagc
     4441 tcggccagga tgcgaagctc cgcctgtgaa taatcgcaag taattagctt gtacccttcc
     4501 gacgccacga agcacttgcg gaaatccgag gtagcaggta tttgctgaac atttggcctc
     4561 gtacaactga agcgccccgt atcagcgccg tgctggttga aatcggggtg gatgcggccc
     4621 gtcttggggt tgatgaggcc cagcacgttc tccccgaagg ccgagagcgt cttttcgtgc
     4681 gacctgtact ccaacaactt cgccacggcg gggtggttaa acttcgacag cgtggcctcg
     4741 ctcgtgtccg ggaggtccac gcccagcttg ctgaacacct ccataagctg cgtggacgag
     4801 ttcaggttga tggagggtat gccgaacatg gtggcttgca tcgtggcggg ctggagcatc
     4861 tcgctcagct ccacggcggt tctctcgcgg tgcagcgcca cgtcctcgat gatcttgcgc
     4921 cacttcaccg tgtcaatctg cacgcccgca agctccaggt cgcccacggc ggcgatgcag
     4981 cggaactcca ggtcggccac gtcttccagc ctgtgcttct taagctcctg cttctgcaag
     5041 cgccagatag ggatcatgat gaacgcgtcg cgggcggcat agtgcaactg ctcgtcggcc
     5101 aggtaggcat cgccgttgtg cgccagcttg tagaacgcct cgcgcaccga cttgtcgagc
     5161 acagccccca cgtagcgcgc cgcgatttgc ttgagcgaaa tctcgcggcc cagtcccgcc
     5221 gtgcatatgc gctccgcgag catcgtgtcg tacatgttcg tgagcgtgat gccgcactgc
     5281 tgcttgagca tctcgtactc gaacttggcg ttctgggcta tcttcagcac gttggggttc
     5341 tcaagcaccg acttgagggc caggggcacc agccgcctgc aatccacgat gtacaccttg
     5401 tcggcggtag acacctgcaa gaggagcagc tcggagatgt gcgggtcgag gcccgtcgtc
     5461 tcggtgtcca cccccagcac cgtctcgtgg gccagcatct cggccacctg ggccaggcgc
     5521 gaagggtcgt ttataagctc gtactcgaag tcgttatagt tgcccgccga agtggccttg
     5581 tcctgggcct ccacggttgc cgagtcgggg acgccattcc ccatatcgct ctcaccgaac
     5641 agcgtcaggg gcaccgtttc cgcaatcgcc aggtccactg cctgtccgcc gtgcgctccg
     5701 tcgccgtgat ggccgttgcc gttgccgttg ccgttgccgt tgttggctcc gttcgacgcg
     5761 ccattggcgg tcccgttgcc ggggacggtg tttgcctggg tgttgcccgc accatcagcg
     5821 tgggtctcgg cagcgtccat gtagtcgaag tagagctgga agtaataatc gtcgccccgc
     5881 ttcgtgtgcc gcttcttggg cacgtaggcc acgccggtct cgacaacggc atccgccgca
     5941 acaagcccct cttgctcgac ggctggggcc tcctcctgcg cgattacctg ggcgggccac
     6001 gaccccatgt ccgcctgcat caaccgcgac tctacgtgct cctggggtag cgcggccccg
     6061 cagtgctcgc acgcggcacg agaaggcatc tcgcctccac tgtagagagg gagggaggaa
     6121 taatgttcgc aggcagtgca gaaaagggtc atgcggattt ggaatttggg agttcggatt
     6181 gcggatttgg tggctgtggt tggacttcta ttatacgcct ttgtggaacg gaagtgcgcg
     6241 aatccacaga tgaaatatgg gccatcccaa gagttggagc gtcggggcga tgttaccaag
     6301 aatagacgga accgccaaga cgccaaggac gccaagaacg cgccaagata aggattgtag
     6361 ctcaagcaag atagaaggct aaggtacgat atgatgtgac cccgccctct ttgtcgtctc
     6421 caaaccttac taccttggcg tggtccttgg cgtcgttggc gtccttggcg gttccgtctc
     6481 tttacttgta tctgttgccg caacgctcaa tgttcgggat ggcctgtgtt gcgagatgcg
     6541 gcttcaatat gtaaacatgc ggtcgtgctc tagcgacagc cggaccagga cgttgggcat
     6601 cgccatctcg cacggcttcc cctctatgtc cgccgcgctc acccctcgtg ccttggagga
     6661 catacccgac aggtagaact tgcccccacc cgccacaatc gcatcgtaca actcccgcag
     6721 cttgccagtg cccagcccca ccaggttgtc gagcaccgcg tcccggataa gctgcaccgc
     6781 gtcccccgca aggaacagcg agaccgtgtg cccttcctct atcgcgctct tagctaccag
     6841 gaaggccagg gcggcgcggg tggggttttc ggggccggta gtcacgtgga ccaatatgct
     6901 agacatcttg tcgcctcctc agcatatttc ttaggacgca tccagcagaa gatatattgc
     6961 cggtctcgta aggcgagatc gcacccgtac gctactgatc gagtggttgc acgagtatac
     7021 caccctcctg cacaaacgag caatcgccgg gaggagtagc ccctggtatg gtcgttctcg
     7081 acacggatta gccctgtgga gccctgtgga ggtgccctgt ggctaaagcc acgggcttac
     7141 aagaggcaaa gtcccctacg gggactgagg aagaggtagt agtctccgta ggagacttcg
     7201 tctcttgcca gcccgcaatt cattggcagg gcacaccccc agga
//
LOCUS       DASRWZ010000591         4815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1144865, whole genome shotgun sequence.
ACCESSION   DASRWZ010000591 DASRWZ010000000
VERSION     DASRWZ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144865"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFH60_09275"
     CDS             <1..606
                     /locus_tag="VFH60_09275"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET6314015.1"
                     /translation="GSSNLVAQVENVRRMGVPAVVAINGFPTDTPAEWALIEKVALEA
                     GAVAAVPSTHYMHGGAGASDLASAVVQATQQPADFRFLYPLDIGIKQKIEAIATQFYG
                     AKDVEYADDAEAKIKLYTHMGFDNLPICMAKTHLSLSHDPKLKGRPTGFTLPVRDVRL
                     SAGAGFIYPLCGTMLTMPGLPSDPAGAHIDIDEHGQVVGLH"
     gene            968..3571
                     /locus_tag="VFH60_09280"
     CDS             968..3571
                     /locus_tag="VFH60_09280"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6314016.1"
                     /translation="MSRHHPRNRITNTVLTCIALCAMVLSMVALGTGAANPSPARAFS
                     PNGLVINEIYPSQASTPQNEYFELYNGGTAPIDLSTYVIYNRDGQTPLSRLDNRTILA
                     GQFRAIGPTQLHTTTIAGSGLDRTDFLGLVDRGTSDTIIDVVNFGNPASPSWPNYEKF
                     SQYFFTSNVPPLPADDGPKALQRWPDGKDTDSGTDFSSTIDRSPDHPSCGDPYEDDST
                     LGSAKEQLPGTEVLHRLCGANDSDFISINMSTSYTYTLQTLNPGSAVDTVLQLYDPSN
                     VLIVTDDNASSRTSTIVFRPTANGLYKARVTHKTGAGNEGPNYLYTFRIDATSANTTA
                     TPVTPTPAGCLDPFEPDNSLQQARGIELNTEQVHVLCIAGSPVDQDWVAFSATSNKLY
                     SMYTKDLASSVDTIISLYDSGGRKLAENDDAVPGQGLASRIDYTFGSTSVYYLQIRDR
                     RGVDGTGYQYTVGLSSTGALPPTITASPSPTFNPNSPTPTPSPCNDSFEPDGVPQEAE
                     LFLIGSSQSHIICPATDADWVRFYARAGKVYTIRTTNLGIGLDTYMFLFDSNASSILA
                     ENDDGGDGSVASRIDFYPLRDDYYYVQVKNAGDIGGPQQTYDLSLSVVSGAPQPPSTA
                     TATGGGPPQPPAAVTPSVGAASPQPTRPPVLTPTQGPIEPTPASVGDVPPPVETKEAE
                     PPEPTKEVLPGPTEPPDTGAGEAGGGEEEEVGPTATTMVVGVPRTGGQEDSQSGAGSQ
                     GGSQSVPKPVPAEDKQAPAVEKPPAAPAPPQFVSLDFRIFYDRNRNDIYEINEGIRGI
                     NVFFVATESAQVTGSAITSDKGIGSLKLPASEHRVQIPYLGIDLKMSKFPARGIASLW
                     LPPVQLPDRVP"
     gene            3623..4597
                     /locus_tag="VFH60_09285"
     CDS             3623..4597
                     /locus_tag="VFH60_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314017.1"
                     /translation="MAQDHSHHDSDAAAPPDRAAAGDVSTTGHSTHDAHDAHGATAGH
                     DDNGHGGHGGHGDDAAEVSTLVPTNWKQLIFPALILLLVAILVAGPLFGAFASRPPAS
                     TTPAPAEQHNGGNEGEGAPTPGSEVESTPHSNAPENPVSQAQPTTTSNTPATEAPPPA
                     ASPTDSALSPSAIATRTAVRVAGEQGDVARVPVQIEFGGATFTVKSGTNLLPDWKPTQ
                     DVGLATWIDGTYANHILYLPFSAENETLFKGAKPGDMVRVVMNTGQVFEFAITRSERV
                     VNGPPTQDGQFTVSTAMAQDHAGVTLFLVGDPAADRAVVQADFTGTIQ"
     gene            4605..>4815
                     /locus_tag="VFH60_09290"
     CDS             4605..>4815
                     /locus_tag="VFH60_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314018.1"
                     /translation="MASQPHVTPQAHLQPGHAILFLGDHTSPDEPGYVRLLGDILARF
                     HPSLNLNLISAGSKGQTAQGLRSRTL"
BASE COUNT          990 a         1697 c         1344 g          784 t
ORIGIN      
        1 ggctcaagca acctcgttgc ccaggtcgag aacgtgcgcc gcatgggagt accggcggtc
       61 gtggcgataa acggcttccc caccgacacc cccgccgagt gggcgctcat agagaaggta
      121 gccctcgaag cgggcgctgt ggctgccgtg ccctccaccc actatatgca cggcggcgcg
      181 ggtgcgagcg acctggcgag cgcggtagtg caggccaccc agcagcccgc cgacttccgc
      241 ttcctctacc ccctggacat cggcatcaag cagaagatcg aggccatagc cacccagttc
      301 tacggcgcga aagacgtgga atacgcggac gatgccgaag ccaaaatcaa gctctacacg
      361 cacatgggct tcgacaacct ccccatttgc atggcgaaga cccacctttc cctgtcccac
      421 gaccccaaac tgaagggcag gccaacaggc ttcaccctgc ccgtgcgcga cgtgaggctg
      481 tcggcgggcg cgggcttcat ctaccccctg tgcggcacga tgctaaccat gcccggcctc
      541 ccctccgacc cggcgggcgc gcacatagac attgacgagc acgggcaggt tgtagggctg
      601 cattagtttt ggcgacaggg cttaattatg ttcggtaagc actcgtatcc gcccttcccc
      661 ggcgactgca gtcgcaggca acagttacga agtctgccag cgcggactat gctagataga
      721 gtagtcgccg tagggcgact ttgtagaggt tgcccgcgac ttgagtcgcc gagcaagggc
      781 gggcgagagt gatcaagccc gctttcggcg agcatcggca ttgacaccct cacgcaaatg
      841 gagtattata gcctgtacgt gtgtggctta ttgagctgca caattactct cacactgggc
      901 gcatggccct acatgccccg ccaattccct ctgattactc cgggcgcaaa ggagaatgtt
      961 gtagcttatg tcgcgccatc acccccgcaa tcgcatcaca aacaccgtcc tcacgtgcat
     1021 cgccctctgc gcgatggtct tgagcatggt cgcgctgggt acgggtgctg ccaacccctc
     1081 cccagcgcgc gccttcagcc ccaatggact ggtcatcaac gagatatacc ccagccaggc
     1141 gagcaccccc cagaatgaat attttgagct ttataacggg ggcaccgcgc ctattgactt
     1201 atccacatac gtcatctaca accgcgacgg ccagacaccc ctctcgcgcc tcgacaacag
     1261 gactatcctg gcgggacaat tcagggctat cggccccacg caactgcaca ccaccaccat
     1321 cgccgggtcg gggctggacc gcacagattt cctgggcctc gtggataggg gcaccagcga
     1381 cacgatcatt gacgtggtga atttcggaaa cccggccagt ccctcgtggc ccaactacga
     1441 gaagttcagc cagtatttct tcacatccaa cgtaccgccg ctcccagccg acgacggccc
     1501 gaaagccctt cagcgctggc ccgacggcaa ggacaccgac agtggcaccg acttctcgag
     1561 cactatagac cgctcgcccg accacccctc ttgcggcgac ccttacgaag acgacagcac
     1621 actgggtagc gctaaagagc agctacccgg cactgaggtc ctccaccgcc tgtgtggggc
     1681 gaacgacagt gacttcatct cgatcaacat gagcaccagc tatacgtaca ccttgcagac
     1741 actcaaccca ggttctgcgg tggatacggt gcttcaactc tacgatccct ccaacgtctt
     1801 gattgtgact gatgataacg cgtcgtcacg caccagcacc atcgtgttcc ggcctaccgc
     1861 caacggccta tacaaagcgc gggtcacgca caagacgggc gcaggcaacg aggggccgaa
     1921 ctatctctac accttcagaa tagacgccac ttccgccaac actaccgcca cgccggtcac
     1981 gcccaccccg gcgggctgtc tggacccctt cgagccggac aactctctcc aacaggcaag
     2041 ggggatagaa ttaaacaccg agcaggtcca cgtcctctgc atagcgggca gcccggttga
     2101 ccaggactgg gtggctttct ctgccaccag caacaagctc tattccatgt acacgaagga
     2161 cctcgcgtcc tcggtagaca ccatcatctc cctgtacgac tccggcgggc gcaagctggc
     2221 cgagaacgac gatgccgtgc cggggcaggg ccttgcctcc aggattgact acaccttcgg
     2281 cagcaccagc gtctactatt tgcagatacg cgacaggcgc ggcgtagacg gcacgggcta
     2341 ccagtacacg gtcggcctct cctcaacggg cgcattgcca ccgaccataa cggccagccc
     2401 cagccctact ttcaacccta acagccccac ccccacgccc tcgccttgca acgacagttt
     2461 cgagccggac ggcgtgcccc aggaggccga gctattcctc atcggtagct cgcagtcgca
     2521 catcatctgc cccgctaccg acgccgactg ggtgcgcttc tacgcccgcg caggcaaagt
     2581 ctatacgata cgcaccacca acctgggcat cggcctcgac acgtatatgt tcctcttcga
     2641 cagcaacgct tcttctatcc ttgccgagaa cgatgacggt ggggacggca gcgtagcgtc
     2701 gcgtattgac ttctaccctc ttcgggacga ctactactac gtgcaggtga agaacgcggg
     2761 cgacatcggc ggcccgcagc agacctatga cctcagcctg agcgtcgtat cgggcgcgcc
     2821 ccagccgcca agcacggcaa ctgctacggg tgggggacct cctcagccac ccgcagccgt
     2881 aacgccgagc gtgggtgccg cgtcgcccca gcccacaagg ccgccggtac tgacccccac
     2941 gcagggtccc atcgagccga cgccagcttc agtaggggac gttccccctc ccgtcgaaac
     3001 gaaggaagca gaaccgcctg agccgaccaa agaggtcctc cccggcccca cagagcctcc
     3061 tgacaccggc gcgggcgaag caggtggcgg tgaggaggaa gaggtcgggc cgaccgccac
     3121 gacgatggta gtaggcgtgc cgaggaccgg cggccaggag gactcgcaaa gtggcgctgg
     3181 gtcgcagggt ggatctcaat ccgtgccgaa gcccgtgccg gccgaggata agcaagcgcc
     3241 agcggttgag aagccgcccg ccgctcccgc cccgcctcag tttgtgtcgc tcgatttccg
     3301 catcttctac gaccgcaacc gcaacgacat ctacgagatc aacgagggca tccggggtat
     3361 caacgtcttc ttcgtggcga ccgagagcgc gcaggtgacg ggcagcgcga ttacctccga
     3421 taagggtatc ggcagcctaa agctcccggc gagcgagcac cgggtccaga taccttacct
     3481 gggcattgac ctcaagatgt cgaagttccc ggcacgcggc atcgcttcac tgtggctccc
     3541 tccggtgcaa ctgccggacc gcgtcccata ggcaccattc ttgcaagcat gtaaagaaac
     3601 cagactatag aggcagaaca gcatggcgca ggaccattct catcacgatt cggatgccgc
     3661 agccccgccc gaccgcgcag ccgcaggcga cgtttcgacc acaggacact ctacccatga
     3721 cgctcacgac gctcatggag ccactgccgg ccacgacgac aacgggcacg gtgggcacgg
     3781 tgggcatggc gacgacgcgg ctgaggtctc cacgctggta cctaccaact ggaagcaact
     3841 gatattcccc gcgctcatcc tgctgctggt agccatactc gtggccgggc cgctattcgg
     3901 ggctttcgct tccaggccgc ccgcttccac gacgcccgcg cccgccgagc agcacaacgg
     3961 tggcaatgag ggagagggtg cccccacgcc cggaagcgag gtagaatcca ctccccattc
     4021 caacgcgcct gaaaacccgg tgagccaggc ccaacccact acaacctcta acacgcccgc
     4081 tacagaggcc cctcctcccg ccgcatcgcc caccgatagc gccctgtccc cctcggctat
     4141 cgccacccgc accgccgtca gggtagcagg cgagcagggc gacgtagccc gcgtgcccgt
     4201 gcagatagag ttcggcgggg cgaccttcac ggtgaaatcg ggcaccaacc tcctgcccga
     4261 ctggaagccc acccaggatg tcgggttggc gacctggata gacggcacct acgccaacca
     4321 catcctctat ctccccttca gcgcggagaa cgaaaccctc ttcaagggcg cgaagccggg
     4381 cgatatggtg cgcgtggtga tgaacacagg gcaggtcttc gagttcgcga taacgcgctc
     4441 cgagcgagtg gtgaacggcc cgcccacgca ggacggccag tttacggtct ctaccgcgat
     4501 ggcccaggac cacgcgggcg tgaccctctt cctggtgggc gaccccgccg ccgacagggc
     4561 ggtggtgcag gcggacttta cgggcactat ccagtagccc tgctgtggcg tcccaacccc
     4621 acgtaacccc tcaagcccat ctccaacccg gacacgccat cctcttcctc ggcgaccaca
     4681 cctccccgga cgaaccgggc tacgttcgtc tcctgggcga catactggcc cgcttccacc
     4741 ccagcctcaa tctaaacctc atcagtgcag gctcaaaggg ccagaccgcc cagggcctac
     4801 gctcgcgcac cctgc
//
LOCUS       DASRWZ010000592         5380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1282248, whole genome shotgun sequence.
ACCESSION   DASRWZ010000592 DASRWZ010000000
VERSION     DASRWZ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282248"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="VFH60_09295"
     CDS             <1..1471
                     /locus_tag="VFH60_09295"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET6314019.1"
                     /translation="WLSSQAGYKIPLNVQFNEPALQEYLGSAVAVEIDQELFEGDVRL
                     NGTEVVALPGKEGRRLDVYGTIVAAREHLAKLEAGAVIDLPVQVTQPAVNANEVEDVR
                     KLLTVRVSSPITATAADKRFTLDPQALVRFTTIERNPDRTATQHVTLGWNDRELKILA
                     DRWASEANRSPQNAKFDWQNGAVSVVRESIEGLEVDPAGVVTAIKERADKVEGREFAM
                     PGKVITPTVSSKDIGALGIKEEIASGTSTFKGSSPERATNIRVAADLLNGTVVPPGGT
                     FSFLETMGGIDEAHGFVPGYVIAAERTQLGVGGGVCQVSTTMFRAAFWSQLDITERNQ
                     HSYRVGWYEANGEPVGFDAAVFDPGVDLKFVNTTPGFILIKAGTTTDTLVVTLYGTKL
                     PGEVKLEGPAISNRVPAPPDVYEVDPRLPRGTKKQVETARGGLDTVITRRVIVPGQPD
                     KVDEYHSHYQAWPNWYIVAAESQVPNGARRPEVAPTAAP"
     gene            1507..1869
                     /locus_tag="VFH60_09300"
     CDS             1507..1869
                     /locus_tag="VFH60_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314020.1"
                     /translation="MPQRFRSRGCLIAALLLAVVALLSCGGLFLLRPQSAPDVVAELK
                     ELPLYPGATAVDFRRPVVDNTPRGGSQGASPGSPVIARDYGEMSFEVQAEPRRVLDFY
                     TGELSTRGWRCDPPGGPP"
     gene            2195..2791
                     /locus_tag="VFH60_09305"
     CDS             2195..2791
                     /locus_tag="VFH60_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314021.1"
                     /translation="MPQRFRSRGCLIAALLFAVVALLSCGGLFLLLRPPSAPDIVAEL
                     KQLPLYPSARNVVFGDSVTDNSFKDARRVRVVGAQGANSSPDIVRGYGEMSFEVQAEP
                     SIVLDFYLREFSKRGWDCDPTGAQGPLVIQMGSCNITGQGPPWPRFTGFAGPGASPPW
                     IALNPTHDLRHAVISAYKPRQGAPTTNVTISYDYVSLH"
     gene            2856..3461
                     /locus_tag="VFH60_09310"
     CDS             2856..3461
                     /locus_tag="VFH60_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314022.1"
                     /translation="MPKRFRSRGCMVVTLLTGVVTLLSCSALFLLAVPPPAPDALAEL
                     KQLTPYPGAQKVSYSQPGNQNIAPGTGVWISSSGSGGSAIYTRSNAQMLVYTGDRLET
                     IFDFYVAELTRAGFDCGAFPPAGRSPGAGGSGLMSRLAHCSKIDQSFPQLVFIGFEGP
                     NAAPPWFEIIRPSTFRSAEVNVNRLQNVTELGIDYYARSMR"
     gene            3702..>5380
                     /locus_tag="VFH60_09315"
     CDS             3702..>5380
                     /locus_tag="VFH60_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314023.1"
                     /translation="MDRKRIVKLGGVAMLIVTMLPVTAAYQAPPADAAPGAAGGEVVA
                     QTRARTNQVPDVPPDTSSFTNVWNRTDSLVASGSVKRSWLWGPQAFWTTRELYLDDPT
                     GTESRLVQYWDKSRMEINNPNGDKNDPFYVTNGLLSVELISGRMQTGNNSFEERKPAQ
                     VNIAGDFDDPTAPTYASFVGKANAGGDHPDPNKMGQKVINTIARDGQVGVDPAKNNVP
                     GVEIAYYDDVTKHNVPKVMWDFLNASGPVRRGNQTVNEPISTPWFYATGRPISDAYWG
                     KIKIAGRPTDALIQAYERRVLTYVPTNPAGFQVEFGNIGAHYRDWRYLSAGKLNDNFP
                     LKGQHIGYGFNVQLYYTDKDRVHQVSRDAGFNWARQQVAWRDIQGKNLLFAWGELDSV
                     VDSAVRNKQKILLSIAKSPDWASPKSKGGMPENPLDFGNFMYMLARRYTGKVQAYEVW
                     NEQNYGPEVGGAVTVAPYVELLKYGYTGVKMADPNAIVLFGGLTPTSVTNNLNLALDD
                     VEYLKQIYAYQGGVVKNYYDVLGAHPGSNANAPDQLWPENPGSGKCPPKYAA"
BASE COUNT         1113 a         1660 c         1690 g          917 t
ORIGIN      
        1 gtggttatcg tcgcaggcgg gctataagat accactgaac gtacagttca atgagcctgc
       61 cctgcaggaa tacctgggta gtgccgtggc agtcgagatc gaccaggagc tattcgaggg
      121 cgacgtgcgc ctgaacggca ccgaggtggt ggcgctgccc ggcaaggagg gccgccgcct
      181 ggacgtgtac ggtaccatcg tggcggcgcg ggagcacctg gcgaagctcg aagcgggggc
      241 cgtaatcgac ctgccggtgc aggtgacgca gcccgccgtc aacgccaacg aagtggaaga
      301 cgtgcgcaag ctcctcaccg tgcgcgtgag cagccccatc accgccaccg ccgccgacaa
      361 acggttcacg ctcgacccgc aggccctggt gcgcttcacc acgatagagc gcaatcccga
      421 ccgcaccgct acccagcacg tgacgctcgg ctggaacgac cgcgagctga agatactggc
      481 cgacaggtgg gcaagtgagg ccaaccgcag cccgcagaac gccaagttcg actggcagaa
      541 cggcgcggta agcgtggtac gcgagtcgat agagggcctg gaagtggacc cggcgggcgt
      601 ggtgaccgcg ataaaggaac gggccgacaa ggtggaaggg cgcgagttcg ccatgcccgg
      661 caaggtgatt actcccacgg tgtcgagcaa ggacataggc gcgctcggca tcaaggagga
      721 gatagcgtcc ggcacctcca cgttcaaagg ctcgtcgccg gagcgggcca ccaacatcag
      781 ggtggccgcc gacctgctca acggtacggt cgtgccccct ggcggcacct tctcgttcct
      841 ggagacgatg ggcgggatag acgaggcgca cggcttcgtg cccggctacg tgatagcggc
      901 ggagagaacg cagctagggg tagggggcgg ggtgtgccag gtgtccacca ccatgttccg
      961 ggcggccttc tggagccaac tcgacataac tgagcgcaac cagcactcct accgcgtcgg
     1021 ctggtacgag gcgaacggcg agcctgtggg cttcgacgcg gcggtgttcg accccggcgt
     1081 ggacctcaag ttcgtcaaca cgacccccgg attcatactc atcaaggcgg gcaccaccac
     1141 tgatacgctg gtcgtgacgc tctacggcac caagctgccg ggcgaggtga agctggaagg
     1201 tcccgccatc agcaaccgcg tgcccgcccc tcccgacgtg tacgaggtag acccgcgcct
     1261 gcccaggggc accaagaagc aggtcgagac ggcgcgcggc ggcctggaca cagtgattac
     1321 gcggcgggtc atcgtgcccg gccagcctga caaggtggac gagtaccaca gtcactacca
     1381 ggcgtggccc aactggtaca tcgtggccgc ggagtcgcag gtgccgaacg gggcgcgcag
     1441 gccggaggtg gcgcccacgg ccgcgccctg atatgcctgt tacacgcccg caaggggaga
     1501 tgagacatgc cgcagaggtt ccgctccagg ggttgtctga tagctgcgct cctgctcgcg
     1561 gtggtggcgc tcctctcttg tggcgggctg tttcttctga ggccgcaaag cgcccccgac
     1621 gttgtcgcgg aattgaagga gttacccctc taccccggcg cgacggctgt agacttccgc
     1681 cgtcccgtcg tagacaacac cccccgaggg ggttcgcagg gggcgtcccc cggtagccct
     1741 gtcatagcac gcgattacgg cgagatgtcg ttcgaggtcc aggctgagcc acgtagggtg
     1801 ctcgacttct acacagggga gctcagtaca agaggctggc gttgcgaccc tccgggggga
     1861 cctccgtaag tgcgatagct gagtgcagga aagcagacga ggggccgccc tggccgaggt
     1921 ttacgggctt cgatgggcct gatgccagcc caccctggat aaagctggac cgggtcgtta
     1981 gctatcacct cgcggcgcta tccgctcacc ggccaaggcc cgatgcctca actaccaacg
     2041 tgaccataac ttacgattac atgtctatac gttagatgga acaaatggct gttgagcagc
     2101 cgcgcccgca aagcatctcc cataaggcta ttgcgctccg aaaagcagcg ctacagtgcg
     2161 tggataacct cccaaatgcc aggataggtg agacatgccg cagaggttcc gttccagggg
     2221 ttgtctgata gctgcgctcc tgttcgcggt ggtggcgctc ctctcttgtg gcgggctgtt
     2281 cctgctgttg cgcccgccaa gcgcaccgga cattgtcgcg gagttgaagc agttgcccct
     2341 gtatccaagt gccaggaatg tggtcttcgg tgactctgtt acagacaata gctttaaaga
     2401 tgcgcggcgg gtcagggtcg ttggcgcgca gggcgcgaac agcagccctg acatagtacg
     2461 tggttacggc gagatgtcgt tcgaggtcca ggctgagccg agcatagtgc tcgacttcta
     2521 cctgagggag ttcagtaaga ggggctggga ttgtgatccc acgggggccc aggggccgct
     2581 cgtaatccag atggggtctt gcaacataac gggccagggg ccgccttggc cgaggtttac
     2641 cggcttcgct gggcctggtg ccagcccgcc ctggatagca ctaaacccaa ctcatgacct
     2701 tcggcatgcg gtgatatccg cctacaaacc gcgccagggt gccccaacca ccaacgtgac
     2761 gatatcttac gactacgtgt ccctgcacta gatggaatct acggctgatg agcagccgcg
     2821 ccggcggagc acccaaaggc caggataggt gagagatgcc gaagaggttc cgttccaggg
     2881 gttgcatggt cgttaccctc ctgactggag tggtgacgct cctatcttgt agcgcgctgt
     2941 tcctgcttgc agtgccgccg cccgccccag atgcgctggc agagctaaag cagctaaccc
     3001 cgtatcccgg cgcgcagaag gtaagctact cccaacccgg caaccaaaac atagcgccgg
     3061 gcacaggggt ttggataagt tcttcaggca gtggtggcag cgcgatttac acgagaagta
     3121 acgcgcagat gttggtttac actggtgatc gcctggagac tatattcgac ttctatgtgg
     3181 cggagctaac gcgggcaggc ttcgactgtg gcgctttccc gcccgcaggc cggagtcctg
     3241 gcgcaggtgg ctcgggactg atgagccgcc ttgcccattg ctctaaaata gaccagagct
     3301 ttccccagct ggtgttcatc ggcttcgagg gacctaatgc cgcgccgccc tggtttgaga
     3361 ttatcaggcc atccaccttc aggagcgccg aggtaaacgt gaacagattg cagaacgtca
     3421 cggagctagg catagactat tacgctagaa gcatgaggta agcgatgtct atgcggaaaa
     3481 cccggcgcgt cgccctctac gtagcataaa atgccaacaa tacgcaaaaa gcattagaac
     3541 ctgggcgcat ttcaagcgtt ataataacgg tactgcatca gaatgcatgc acgacacgta
     3601 gtatgcccac aactaccgtg aggtaatcgg cgtttcagtt tgccccgcga aacgcccacg
     3661 ttctcccagc gattcgaacg taggaacata ggaggataga cttggacaga aagaggatcg
     3721 tcaagcttgg cggcgtcgcc atgctcatcg tcaccatgct gcccgtgacg gcagcgtacc
     3781 aggcaccacc ggctgatgcc gcgccgggag ctgcgggcgg cgaggtggtc gcccagacca
     3841 gggcgcgcac caaccaggtc cctgacgtac cgcccgatac gagttccttc accaacgtgt
     3901 ggaaccgcac cgactcgctg gtggcttcgg gttccgtcaa gcggtcgtgg ctgtgggggc
     3961 cgcaggcgtt ctggaccacc cgcgagcttt acctggacga cccgaccggc accgagagcc
     4021 gcctggtcca gtactgggac aagagccgca tggagatcaa caaccccaac ggcgataaga
     4081 acgacccgtt ttacgtgacc aacggcctcc tctcggtcga gcttatctcc ggcaggatgc
     4141 agacgggcaa caactccttc gaggaacgca agcccgccca ggtgaacatc gcgggcgact
     4201 ttgacgatcc caccgcgccc acttacgcct ctttcgtcgg caaggccaac gcgggcggcg
     4261 accaccccga ccccaacaag atggggcaga aggttatcaa caccatcgca cgcgacggcc
     4321 aggtgggggt tgaccctgcc aagaacaacg tgcccggcgt cgagatcgcc tactacgatg
     4381 acgtgaccaa gcacaacgtc cccaaggtga tgtgggactt cctcaacgcc tctggcccgg
     4441 tgcgcagggg caaccagacg gtgaacgagc cgatttcgac cccctggttc tacgccacgg
     4501 gccgccccat ctcggacgct tactggggca agataaagat cgcgggcagg cccaccgatg
     4561 ctctcatcca ggcgtatgag cgccgcgtgc tcacctacgt gcccaccaac ccggcgggct
     4621 tccaggtgga gttcggcaac atcggcgcgc actaccgcga ctggcgctac ctgagcgcgg
     4681 gcaagctcaa cgacaacttc ccgctcaagg gccagcacat cggctacggc ttcaacgtac
     4741 agctttacta cacggacaag gaccgcgtgc accaggtgtc gcgagacgcg ggcttcaact
     4801 gggcacggca gcaggtcgcc tggcgcgaca tccagggcaa gaacctgctc ttcgcctggg
     4861 gcgagcttga ctcggtggtg gactcggcgg tacgcaacaa gcaaaagatc ctcctgtcca
     4921 tcgccaagtc gcccgactgg gcctcgccca agagcaaggg cggcatgccg gagaacccgc
     4981 tcgacttcgg caacttcatg tacatgctgg cccgccgcta cacaggcaag gtgcaggctt
     5041 acgaagtttg gaacgagcag aactatggac ctgaggtcgg cggtgcggta acggttgccc
     5101 cgtacgtaga actgctcaag tacggttaca ccggggtcaa gatggccgat cctaacgcta
     5161 tagtgctgtt cggcggtctg acaccaacga gtgtaacgaa caatctgaac cttgccctcg
     5221 atgatgtaga gtacctcaag caaatttacg cgtaccaggg tggcgtggtc aagaattact
     5281 acgacgtgct gggagcacac cccggctcca atgccaacgc gcccgaccag ctttggccgg
     5341 aaaaccccgg tagtgggaaa tgtccgccca agtacgccgc
//
LOCUS       DASRWZ010000593         3872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1144940, whole genome shotgun sequence.
ACCESSION   DASRWZ010000593 DASRWZ010000000
VERSION     DASRWZ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144940"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..2075)
                     /locus_tag="VFH60_09320"
     CDS             complement(75..2075)
                     /locus_tag="VFH60_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314024.1"
                     /translation="MPDVVCTTCGATNRGTSHFCARCGQPLPPAEEVGGAQDAQAAGE
                     PGQGAQGPQGKPDAPAAGVDLPWLQAVQQHSVQETSDLRRTPPPATEPPRPPVQPTQP
                     TQPTPPPPGPESQPPVEQAASQTPEAEQMPPNVRQLPVREPAQAPQQPEQGAQVPPAD
                     PNEPPPDWVVGILEPSVTPPPPQQEYEPEELAHIMPWVHPDPDQGAEGPPNPAAGSQL
                     PPWLREVTVQETLQAVPPTPQEETVAPTGYEDYALEGIEPFVPPPIAEEEAAQPQAPR
                     QEEQVPAWLRAIPGGSDERGQALVDAGVTVPDYSAQAALDAMPAISEGGARNIPVRAP
                     RVGAVDTLAALLQAEAHNMPRRVPSGMTGMLVLDEPTARRGFTRWLMPDGLIYLLVLG
                     VLLAILLVRPPFGDIPFAPSTDVTQFYNAVESVPQDRPVLVVYDWDATRSAEMSVLSQ
                     AVMRHLMSRRLQFVTISTAPQGPGFAQQVVRAALADPVSNYGYTYGTDYLILGYIPGN
                     EAALRSAVDDFTAVLPLDYQQIQRLDTYPILQQGNIKGARDFALVIDLASDEAELRNW
                     IEQVGARTGVPIIAAVPQSLDPIARSYNRVRGTGLQAVVSGTDGALQYNRQLLQAGRN
                     TGQMNIDALTDRLNAQSVAQLLVALVIVAAFISMATRRIFRR"
     gene            complement(2141..2779)
                     /locus_tag="VFH60_09325"
     CDS             complement(2141..2779)
                     /locus_tag="VFH60_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314025.1"
                     /translation="MTNRIGSLLILILNLALAYVAGVISIVGFFTQGTFSEMGGTLAQ
                     WVAVLVGFALLVGLGNRIKVHLTRVARREEGWLYSLVLVGSALVVVGMGLMGRGPGDA
                     SVAWVFQWIYQPLGQAVFSLLAFFLVTALFRSTRLRNAEALVLLGVALLVIIGQAPIS
                     SLPPLDVMVGVKDWILEYPALAGIRAILLGVSFGAIAMSLRILLGFDRPYLD"
     gene            complement(2852..3685)
                     /locus_tag="VFH60_09330"
     CDS             complement(2852..3685)
                     /locus_tag="VFH60_09330"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HET6314026.1"
                     /translation="MDFSNAVPAYGDALYIMLGVLVAFLLLFVGFLWRAGWGRRGGTR
                     RAITLRHIPGYDATRLGLSTAAETGNAMHTSPGSGGVGSRGVNTAATLAGMNLVESMS
                     RVGAITGAPVQATTNDAIAYALTDNAVRRGYRRAGWSMESESAGARFLTHNDPLAYAA
                     HVTDIVDRSRVSQAVMAGHFGPEVLLITEAQRRTGAYQISGSSDPQALALMTLTADHT
                     LVGEEIFASGAYLERRTSHIASLLAQDGLRWLVILLIIAGFILVNVLGQNWFDILTLY
                     P"
     gene            complement(3685..>3872)
                     /locus_tag="VFH60_09335"
     CDS             complement(3685..>3872)
                     /locus_tag="VFH60_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314027.1"
                     /translation="WLGGQMLCVFQPFAGAFGIGARKGPLSIEGVARILEREGGTEAL
                     VERLDAGIQARERGGQR"
BASE COUNT          668 a         1280 c         1270 g          654 t
ORIGIN      
        1 tggtcagtgg tcagtggtcg gtggtcggtt ggtcttctta ctgaccaccg accactgacc
       61 actgaccact ggcgctacct cctgaagatt cgcctcgttg ccatgctgat gaaggcggcc
      121 actatcacca gggccaccag cagttgagct accgactggg cgttcaggcg gtcggtgagc
      181 gcgtcgatgt tcatctggcc ggtgttgcgg cctgcctgta gtagctggcg gttgtactga
      241 agcgccccgt cggtgccgct caccaccgcc tgcaaccccg tgccgcgcac ccggttgtag
      301 ctgcgggcga tggggtcgag actctgcggc acggcggcta tgatgggcac acccgtgcgc
      361 gctcctacct gctctatcca gttgcgcagc tccgcttcgt ccgaggccag gtcgatcacc
      421 agggcgaagt cgcgtgcgcc cttgatgttg ccctgctgca agatcgggta ggtgtcgagg
      481 cgctgtatct gctggtagtc gaggggcagc accgccgtga agtcgtctac tgccgagcgc
      541 agggccgctt cgttgccggg gatgtagccc aggatgaggt agtcggtgcc gtaggtatat
      601 ccgtagttgg ataccgggtc ggccagcgcc gcacgcacca cctgctgcgc aaagccgggg
      661 ccttgcgggg cggtgctgat cgtcacgaac tggaggcggc gcgacatcag gtggcgcatg
      721 accgcctgcg acagcaccga catctcggcg gagcgcgtgg cgtcccagtc gtacaccacc
      781 agcacgggcc ggtcctgggg caccgactcc acggcgttgt agaactgcgt cacatcggtg
      841 ctgggggcga agggtatatc cccgaagggc gggcggacca gcaatatagc cagcaacacg
      901 ccgagtacca gcaggtaaat cagcccgtcc ggcatcaacc accgggtgaa gccgcgtcgt
      961 gcggtaggtt cgtccagcac cagcataccg gtcataccgg agggcacccg gcgcggcatg
     1021 ttatgcgctt cggcctgcaa caatgcggcc agcgtatcga cagcccccac cctgggagcg
     1081 cgcacgggaa tgttgcgggc cccaccctcc gaaatagcgg gcatcgcatc cagtgcggcc
     1141 tgcgccgagt aatcgggcac cgttacgcct gcatctacca gagcttgccc gcgctcgtcc
     1201 gacccacccg gaatagcgcg cagccaggcg ggcacctgct cctcctgcct gggggcttgc
     1261 ggctgggcgg cctcttcttc cgcgatgggg ggaggcacga acggctctat accctcaagc
     1321 gcgtagtcct cgtagcccgt gggggcgacc gtctcctcct gcggtgtggg aggcaccgct
     1381 tgaagggtct cctgcacggt gacctcgcgc agccagggcg ggagttggct tcctgcggcc
     1441 gggttcggcg gcccctcggc accctggtcg gggtcggggt gcacccacgg catgatgtgg
     1501 gcaagctcct ccggctcgta ctcctgctgc gggggaggcg gggtcacgct cggctcgagt
     1561 atgcctacga cccagtcggg gggtggctca ttggggtcgg cggggggcac ctgggcaccc
     1621 tgctccggct gctggggagc ttgggccggc tcgcgcaccg gcaactggcg cacgttgggc
     1681 ggcatctgct cggcctcggg cgtctggctc gccgcctgct ctaccggggg ctgagactca
     1741 gggccgggtg gcggaggagt aggctgagtg ggctgagtgg gctgaaccgg aggccggggc
     1801 ggttccgtgg cgggaggtgg ggtccggcgc aggtcgctgg tctcctggac cgaatgctgt
     1861 tgcaccgcct gcaaccacgg caagtccacc ccggcagcgg gcgcgtctgg cttgccctgc
     1921 ggaccctgcg caccctgccc cggctcgcct gcggcctggg catcctgcgc cccaccaacc
     1981 tcctcggcgg gaggcaaagg ctggccgcac ctggcgcaaa agtggctggt gccgcgattg
     2041 gtagccccgc aagttgtaca aactacatca ggcatatcgc attttggatt ttggattttg
     2101 gatttaggcc cttaagcaat ctaaaatcca aaatctaaaa tcagtccaga tagggtctgt
     2161 cgaagcccaa caatattctc aggctcatgg ctattgcgcc gaaggagacg cccagcagta
     2221 tggctcggat gccggccaga gccgggtact ccaatatcca gtctttgacc cccaccatca
     2281 cgtcgagcgg ggggaggctg ctgatagggg cctggccgat tatcaccagc agggccacgc
     2341 ccagcagcac cagggcctcg gcgttgcgca ggcgggtgga gcgaaacagg gccgtgacca
     2401 ggaagaaggc cagcagcgag aagacggcct gccccagcgg ctggtagatc cactggaaca
     2461 cccaggctac cgaggcatct cccggcccgc ggcccatgag gcccataccc actaccacca
     2521 gcgcgctacc caccagcacc aggctgtaga gccacccctc ctcgcgccgg gccacccgcg
     2581 tgaggtgcac ctttattcga ttgcccagcc ccaccagcag cgcgaacccc accagcacgg
     2641 ccacccactg cgccagcgtc ccgcccatct ccgagaaggt gccctgcgtg aaaaaaccca
     2701 cgatgctgat taccccggcg acgtacgcca gggccaggtt cagtataagt atcagtagag
     2761 agccgattct gttggtcatt tggattttgg attttggatt tcggattttg gattaggtct
     2821 gaagcaatct aaaatccaaa atctaaaatg gtcacgggta cagggtcagt atgtcgaacc
     2881 agttttgccc cagcacgttc accaggatga acccggcgat gatgagcagg ataacgagcc
     2941 agcgcaggcc gtcctgggcg agcagggagg ctatgtggct ggtgcggcgc tccaggtacg
     3001 cgcctgaggc gaatatctcc tcacccacca gcgtgtgatc cgccgtgagc gtcatcaggg
     3061 cgagggcttg cgggtcggac gagccagaga tctgataggc ccccgtacgc cgctgggcct
     3121 cggtgatgag gagcacttca ggcccgaaat gccccgccat gacggcctgc gacaccctgc
     3181 tgcggtccac tatatcggtg acgtgggcgg cataggccag cgggtcgttg tgggtgagga
     3241 aacgcgcccc cgcgctctcg ctctccatcg accagcccgc ccgcctgtag ccccggcgca
     3301 ccgcgttgtc ggtcagggcg taggcgatag cgtcgttggt ggtggcctgc acgggcgctc
     3361 ccgtaatcgc ccccacccgc gacatcgact cgaccaggtt catgccggca agcgttgcgg
     3421 cggtgttcac gcccctggag ccgacgccgc cggagccggg ggaggtgtgc atagcgttgc
     3481 ctgtctcggc ggcagtggac agtcccaggc gggtggcatc gtagccgggg atgtggcgca
     3541 aagtgatagc ccgcctggtg ccgccacgcc tgccccagcc cgcccgccac aaaaacccca
     3601 cgaagagcaa caggaaagcc acgagcacgc ccagcatgat gtacagggca tcgccgtaag
     3661 ctgggacggc attgctaaag tccatcagcg ctgccctccc ctctcgcggg cctgaatccc
     3721 ggcgtccaac cgctccacca gggcctccgt gccgccctcg cgctccaata tgcgagccac
     3781 accctctata gagagcggcc ccttacgcgc cccgatgccg aaggcccccg cgaacggctg
     3841 gaacacacac agcatctgcc cgcccagcca cg
//
LOCUS       DASRWZ010000594         3728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_939208, whole genome shotgun sequence.
ACCESSION   DASRWZ010000594 DASRWZ010000000
VERSION     DASRWZ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939208"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..1896
                     /gene="purF"
                     /locus_tag="VFH60_09340"
     CDS             331..1896
                     /gene="purF"
                     /locus_tag="VFH60_09340"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET6314028.1"
                     /translation="MGEMLSSQVAYEDDKLHEECGVFGVWAPGEDVARLTYFGLYALQ
                     HRGQESAGIASTEGGRLHMRTRMGLVSTSFDEEGLAKLKGHAAIGHTRYSTTGSSKLC
                     NAQPLRFDDATLGWLALGHNGNVTNATAIRGELQELGVPFDTTSDSEVIGKLAMSSHG
                     NNWVSRFRRAMSRLTGAYSLVLLSPTHLMAVRDPMGIRPLCLGKLPGGGWIVSSETCA
                     LMTVGAQFIREIAPGEIVTIGVEGLTSYADVPHVQQAMCVFEYIYFARPDSVIENVPL
                     YTARQNMGRELAREHPADADIVIGVPDSATPAAIGYSMESGIPYTEGLIKNRYIGRTF
                     IQPHQHLRERGIGLKFNPLPGVLSGKRVVVVDDSIVRGTTTKPIVELLKRAGATEVHV
                     RIHSPAMSHPCYLGVDTARREELIAHRLSVQEIALHIGADSLGYLSLPGLFRAVGLGD
                     GKLCSGCFTGNYPVPVQLEFAGLDGKLAMEGVETARGFSAREHEAIIQGQLELSFDQA
                     EMDRDRVPAGVDR"
     gene            2045..2680
                     /locus_tag="VFH60_09345"
     CDS             2045..2680
                     /locus_tag="VFH60_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314029.1"
                     /translation="MQKIAPFQVRAMLLSVVLVLLLAACGDPTPQPTPAGVNPTTDAI
                     PAPTTTTPPLPNPTTDAIPAPTATTRLLPPTPEPTNTTSPLALDLSSLDVCTFVPRET
                     LEGIIGPLKDEGTQDTVLGKEKGCTFYNEEGNFADIRIYPPDSWELQKNLQTGLIELH
                     DLGSEGFVVNKSDAYEVWTLARDVAVIQVRVSNHNGGQARQIAEAVLDLLP"
     gene            2948..>3728
                     /locus_tag="VFH60_09350"
     CDS             2948..>3728
                     /locus_tag="VFH60_09350"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6314030.1"
                     /translation="MEINRERLQLIEGIADERVRAGNMPCAVVAVANSRETLWTHVVP
                     GDEGVGLDSIFLLASITKPIVATAIMRLVEEGRLMLNVPVAHYLPEFGTNGKEKVTAF
                     HLLTHSSGMEEETFWNELWALPGTLPPPEWQYDACCRSYLHYEPGTQHTYSTLPFSVL
                     AELVKKLGGVPHAEYLRRHIFEPLGMHSTGYRPTDPTKAAPVHKLIGEAETTPELLAA
                     FMSFEQPGSGLWSTAADLVTLGQAFLRGGTYEGYHLLSPVTI"
BASE COUNT          716 a         1202 c         1151 g          659 t
ORIGIN      
        1 cactgtcgag aagtggccgg agctgcaagc cctggcgcgt tcttatggag tggacctatt
       61 cctgctgggc ctcagcgggg gcgaccaact gaccgtcact cgcgccgaca accgcgttgt
      121 ggtagacttg cccctgcctg aggtggacac cgcatggcgc aaaggtctgg gctgaggggc
      181 gcagggtgct ccctgccggg gggttggggg tgtcccccaa ttcctctctt tctcattctt
      241 ttttagattt ttcataaatg gtcgggctaa ggcacatcaa agtaacagag caggcgcggt
      301 cgcgcatcat ggcagtagag gggtaacgac atgggtgaga tgctgagttc gcaggtcgca
      361 tacgaggacg acaaactgca cgaggagtgc ggcgtgttcg gcgtatgggc accgggagag
      421 gacgtggcga ggctcaccta cttcgggctg tacgctttgc agcaccgggg gcaggagtcc
      481 gccggtatcg cctccaccga gggcggcagg ctgcacatgc gcacccgcat gggcctcgta
      541 tcgacctcgt tcgacgagga agggctggca aagctcaagg gccacgccgc gataggccac
      601 acccgctact cgaccaccgg ctcctccaag ctgtgcaacg cccaacccct gcgcttcgat
      661 gacgccacgc tcggctggct ggcgctgggg cacaacggca acgtcaccaa cgccacggct
      721 atacgcgggg aattgcagga gctgggcgtg ccgttcgaca ctacctccga cagcgaggta
      781 atcggcaagc tggcgatgtc ctcccacggc aacaactggg tgagccgctt ccgccgggcg
      841 atgtcgcgtc ttaccggggc ctattcgctg gtcctcctgt cgcccactca cctcatggcc
      901 gtgcgcgacc cgatgggcat ccggcctctc tgcctgggca aactgccggg cggcggctgg
      961 atcgtgtcgt ccgagacgtg cgccctgatg acggtgggcg cgcagttcat ccgcgagata
     1021 gcgcccggcg agatcgtcac gattggcgtg gagggcctta cctcttacgc tgacgtgcct
     1081 cacgtgcaac aggccatgtg cgtgttcgag tacatctact tcgcccgccc cgacagcgtg
     1141 atagagaacg tgcccctcta caccgcccgc cagaacatgg gccgcgagct tgcgcgggag
     1201 caccccgccg acgccgacat cgtgataggc gtgcccgact cggctacccc cgccgctatc
     1261 ggctactcga tggagtcggg cataccttac accgagggcc tcatcaagaa ccgctacatc
     1321 ggccgcacct tcatccagcc gcaccagcac ctgcgcgagc gaggcatcgg cctcaagttc
     1381 aaccccctgc cgggcgtgct cagcggcaag cgcgtggtgg tggtggacga ctccatcgtg
     1441 cgcggcacca ccaccaagcc gattgtggaa ctgctcaagc gcgccggggc caccgaggtg
     1501 cacgtgcgca tccactcgcc cgccatgtcc cacccctgct acctgggcgt ggacaccgcc
     1561 cgccgcgagg agcttatcgc gcataggctg tcggtgcagg agatagccct gcacatcggg
     1621 gccgactcgc tcggctacct gtcgctgccg ggcctcttcc gcgccgtggg gctgggcgac
     1681 ggcaagctct gctccggctg cttcaccggc aactaccccg tgcccgtgca gttggagttc
     1741 gcgggcctcg acggcaagct tgcgatggaa ggcgttgaga ccgcccgtgg cttctccgct
     1801 cgcgagcacg aggcgatcat ccagggccag ctagagctat cgttcgacca ggccgagatg
     1861 gaccgcgaca gggtgcccgc cggcgtggac cgctaaccag cccgcctcct cagatttgct
     1921 ccctctttcg caggaatggt tcatgctaca gaagcagtgt gtttgctgca ccctctacgg
     1981 gccacagggc gctgtgacgt agacgtcggc agacctgtac ccctagcttc ggaggaacct
     2041 tacaatgcaa aagatcgccc ccttccaggt gcgggctatg ctgctttccg tcgtgctcgt
     2101 gttgctgctg gccgcgtgtg gcgaccccac gccgcagccc acacccgctg gagtcaaccc
     2161 aaccaccgac gccatccccg ctccgaccac cactacaccc cctctaccca atccgactac
     2221 cgatgccatc cccgccccca ccgccaccac tcgcctactg ccacccactc ccgaacctac
     2281 aaacaccacc tcgccgctcg cgctcgacct gtcctccctg gacgtttgca ccttcgtccc
     2341 cagggagacc cttgaaggca taattggccc gctgaaggac gagggcaccc aggatacggt
     2401 gttgggcaag gagaagggct gtaccttcta caacgaagag gggaacttcg cagacataag
     2461 gatttacccg cccgacagct gggagttgca gaagaactta cagaccggcc tgatagaact
     2521 tcacgacctg gggagcgagg gatttgtcgt caataagtcc gacgcgtacg aagtgtggac
     2581 gcttgcacga gacgtggccg tgatacaggt gagagtaagc aaccataacg gcggacaggc
     2641 gaggcagata gcggaagcag tgctagacct actgccctag cgcctatctg caaacagcag
     2701 gaagtaagcg accaaagcta gaccttgtca ttctgagcgc agcgaagaat ctcgtcctct
     2761 agcacccaaa taccacttta cttccaatgg gtaaggtgcc ctgtgctacg gaaaagggga
     2821 acacctgcat ggaggacgag attattcgct gcgctcagaa tgacaagggg gacgagggtt
     2881 cagtacattc ggcacctggc agataggccc tggttgagcc gtgcaataca gaggaactga
     2941 gggagtcatg gagataaatc gggagcggct tcagcttata gagggtatcg ccgatgagcg
     3001 cgtgcgggcc ggcaatatgc cttgcgcggt ggtggcggtc gccaacagcc gggagacgtt
     3061 gtggacgcac gtcgtgccgg gagacgaggg ggttgggctg gacagcatat ttctgctggc
     3121 ttcgataacc aagcctatcg tggctacggc gattatgcgg ttggtggagg aaggcaggct
     3181 gatgctcaac gtgccggtcg cccactacct gccggagttc ggcactaacg gcaaggagaa
     3241 ggtgaccgct tttcacctgc tcacccactc cagcggcatg gaagaagaga ctttctggaa
     3301 cgagctttgg gcgttaccag gcacgctacc cccgcccgag tggcagtacg atgcctgctg
     3361 ccgcagctac ctgcattacg agccgggcac acagcatacc tactctacgc tgccgttcag
     3421 cgtgcttgcc gagcttgtca agaagttggg cggcgtgccc cacgccgagt acctgcgccg
     3481 ccacatcttc gagccgctgg gcatgcacag caccggctac cggcccaccg accccacgaa
     3541 ggcggccccg gtgcacaagc tcattggaga ggcggagacc acgccggagc tattggcagc
     3601 tttcatgtct ttcgagcagc cagggtcggg cctgtggagc acagccgccg atctggtgac
     3661 gctggggcag gcattcctgc ggggcggcac ttacgaaggg tatcacctgc tctcgcccgt
     3721 cactattg
//
LOCUS       DASRWZ010000595         4812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_802243, whole genome shotgun sequence.
ACCESSION   DASRWZ010000595 DASRWZ010000000
VERSION     DASRWZ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802243"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..142)
                     /locus_tag="VFH60_09355"
     CDS             complement(20..142)
                     /locus_tag="VFH60_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314031.1"
                     /translation="MLEHRQNVARVARAQVLELEGRDAPAGHVEAPVEAEHQLL"
     gene            131..334
                     /locus_tag="VFH60_09360"
     CDS             131..334
                     /locus_tag="VFH60_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6314032.1"
                     /translation="MFEHNEHDLLTLVALTGHVGNILDSPDEADLHAAEWFGLGKLFE
                     DLGRYTDSLRAYSRGLDFDPSPE"
     gene            341..652
                     /locus_tag="VFH60_09365"
     CDS             341..652
                     /locus_tag="VFH60_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314033.1"
                     /translation="MALRRLSFLDKYLQQQEEAVAIWNRLAAEGTHLMFPYLELAKHY
                     EHRVKDYAAAAEYTLLAQVRATNAVERAELQHRLDRVAAKARRHAEREAPRPAPQQPQ
                     A"
     gene            665..2065
                     /locus_tag="VFH60_09370"
     CDS             665..2065
                     /locus_tag="VFH60_09370"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6314034.1"
                     /translation="MDTLPTVTLVLAANDLLLIPPQGTTPEELRAALHHTGWQGRTVL
                     DSRRNGLRALPLDYTAICEALAGRYNLKPLFELRPGLPHTVEVRQQPRPYQRNAIEAW
                     REAGHRGVVVLPTGAGKTLVALLCIAELNTQALICVPTLDLLGQWKTSILSNTNLRPE
                     EVGTWGGGDKELQPVTVITYDSAAIHMRSIQGFGLLVFDEVHHLPADTYRTIAEGSIA
                     TARLGLSATPDRSDLRHTDLDRLVGPVVYERSPFQLREGKHIADYRTEQISVALTGDE
                     RTAYERAAEVYRAYLRKNRISMRSGSDYERFLIWRSGNDHGARDALLAHQAARKIALS
                     ASGKLQVVAGLLARHADDRVLVFSEYNALVDEIGKRFCIPTVTHKTPPAERKAVLEGF
                     RSGRFSKLATGRVLNEGVDLPDANVAIILSGSATKREFIQRLGRVLRPKTTEAVLYEV
                     VTEETTEENISRRRQG"
     gene            2158..3951
                     /locus_tag="VFH60_09375"
     CDS             2158..3951
                     /locus_tag="VFH60_09375"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HET6314035.1"
                     /translation="MLFSLTNVKRRSVRDADGELAVVPKLLHGRSSLKLVEQAIATFE
                     QCVGRPRAEYDSRALEAVMGDYRLGRCIEACLLTRYGFVQPDIADILPPGEVAALKSL
                     GLDSPGALRLALWDAANERGGFVAPEEREAFLNRLAAEWGLCEPARIDALVSLDTESA
                     AVLAPTADKPTAAEMLTLYNRGMVKTLLAHSTQVRFNMGSLPGDVLRRLYFIAKRSGV
                     LVDVESDRAGGFSLTLFGPEQAFGSADKYGRRLADVSLSLLRSLLTLPPSTPVQGTAS
                     LVLHDRTYRFHLTQDILDHLEYAPEPQAGARTGRVAERSAAYSVGSSLDHEGDDAIED
                     LEPTFDSLVEASLYRGFRSLEKQGDTHGWLILREPDPLLAPGVVLIPDFAFERGGTRV
                     YMEVAGFWSPSYKERKVAKLHALGASGEDTALILAVPKDSAPAFTGLPFPIVPYKKDI
                     RATDMLALLDAQYGAREERLTSARGRFAPLQQAALKRGLVPERDLAEALQAYSRSELL
                     AVAPELDTPGCRYVPGVGLLSDAAADRAHTSLLAALEASPERRIDLDQAVELVSSALG
                     ASHVDVEALLQLWPQFRVDRPSLFEAFIALA"
     gene            4415..>4812
                     /locus_tag="VFH60_09380"
     CDS             4415..>4812
                     /locus_tag="VFH60_09380"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HET6314036.1"
                     /translation="MNPLPGRCPVCANSLTVTRLQCGHCGTGIDGAFGLGRLQALTAE
                     QVQFVETFMKCKGKIKDVEGELGISYPTVVARLNEVVRAMGFEVDEGDLSDVDQYEYY
                     QARVLSPQMPARPAPPSPISPMPSMPVVPPV"
BASE COUNT          908 a         1700 c         1387 g          817 t
ORIGIN      
        1 ctcctgctcc cttgacacgt tagagcaact ggtgctcggc ttcaaccggg gcttcgacgt
       61 gcccggctgg cgcatcccgt ccctctagtt ccagtacctg cgcgcgggcc acgcgggcca
      121 cattctgccg gtgttcgagc acaacgagca cgacctgctc accctcgtgg ccctcaccgg
      181 ccacgtgggc aacatcctgg acagccccga cgaggccgac ctccacgccg ccgagtggtt
      241 cggcctcggc aagctgttcg aggacctggg ccgctacacc gacagcctcc gtgcctactc
      301 taggggcctc gacttcgacc cctcgcccga atagcgcggt ttggccctgc gccgcctgag
      361 cttcctcgac aagtacctgc aacagcagga agaagccgtg gcgatctgga accgactggc
      421 cgccgagggc acccacctga tgttccccta cctggagctt gccaagcact acgagcaccg
      481 cgtcaaggac tacgccgctg ccgccgagta caccctgctc gcccaggtcc gcgccactaa
      541 cgcggtggag cgtgccgagt tgcagcaccg cctcgaccgg gtagccgcca aggcccgccg
      601 ccacgccgag cgcgaagcgc ccagacctgc cccacagcag ccccaggcat agaaagcggc
      661 ccccttggac acactcccca ccgtaaccct ggtcctcgcc gccaacgacc tgctcctcat
      721 cccgccccag ggcaccacgc cggaagagtt gcgcgccgcc ctccaccaca ccggctggca
      781 gggccgcacc gtcctagact cgcgccgcaa cggcctgcgc gccctgcccc tcgattacac
      841 ggccatttgc gaggccctag ccggccgcta caacctcaag ccgctgttcg agttgcgccc
      901 cggcctgccc cacacggtag aggtccggca gcagccccgg ccttaccagc gcaatgccat
      961 cgaagcgtgg cgagaggcgg ggcaccgggg cgtggtggtg ctgcccaccg gcgcgggcaa
     1021 gacgttggtg gccctcctgt gtatcgcgga gttgaacacc caggccctca tctgcgtgcc
     1081 tacgctcgac ctgctgggcc agtggaagac gtccatcttg agcaacacca acctcaggcc
     1141 ggaggaagtg ggcacctggg gcggtgggga caaagagttg cagcccgtaa cggtcatcac
     1201 ctacgattcg gccgccatcc acatgcgctc catacagggc ttcggcctgc tcgtgttcga
     1261 cgaggtccac cacctgcccg ccgataccta ccgcaccata gccgaaggct ccatcgccac
     1321 cgcccgcctg ggcctaagcg ccacccccga caggagcgac ctgcggcaca ccgacctgga
     1381 ccggctggtc ggccccgtcg tctacgagcg ctcgcccttc cagctacgcg aaggcaaaca
     1441 catcgctgat tatcgcaccg agcaaatcag cgtggccctc accggcgatg agcggacggc
     1501 ttacgagcgt gcggcggagg tctaccgggc gtacctgcgc aagaaccgta tcagcatgcg
     1561 ctcaggcagc gactacgagc gcttcctcat ctggcgcagc ggcaacgacc acggggcccg
     1621 cgatgccctc ctcgcccacc aggctgcccg caagatcgca ctgagcgcct ccggcaagct
     1681 gcaagtcgtg gccgggctac tcgcgcggca cgccgacgac cgcgtgcttg tcttctccga
     1741 gtacaacgcc ctggtggatg agattggcaa acgcttctgc atacccaccg tcactcacaa
     1801 gacacccccc gccgaacgca aagctgtcct cgagggcttc cgcagcggcc gcttcagcaa
     1861 gctggccacg ggccgcgttc tcaacgaagg cgtagacctg cccgacgcca acgtagccat
     1921 catcctgagc ggcagcgcca ccaagcgcga gttcatccaa cgcctcggcc gagtgctccg
     1981 ccccaagacc accgaagccg tcctctacga agtggtcacg gaggagacga cggaggaaaa
     2041 tatctcccga aggagacagg ggtgacggaa tttggaaatt gggatctcga attgcggatt
     2101 tgctcggata tctactaaat tcgaaattcg aaatcccaat ttccaaatgg aggaatcttg
     2161 cttttctcac tcaccaatgt aaaacggcgc agcgtgcgcg acgcggatgg ggagcttgcg
     2221 gtggtgccca agctgctgca cgggcgctcg agcttgaagt tggtggagca ggcgattgcg
     2281 acgttcgagc agtgcgtcgg gcggccgcgt gctgagtatg actcgcgcgc cctggaggcg
     2341 gtgatgggcg actaccgcct gggacgctgc atcgaggcgt gcctgctcac acgatatggc
     2401 ttcgtgcagc ccgatattgc ggacatactg ccaccgggtg aggtagcggc tcttaaatcc
     2461 ctgggcctgg actcgcccgg tgccctgcgg ctcgccctct gggacgctgc taacgagcgc
     2521 ggcggcttcg tagctcctga ggaacgtgag gcattcctga accggctggc ggctgaatgg
     2581 ggcctatgcg agcctgcccg catcgatgcc ctggtgtcac ttgataccga atcggcagcc
     2641 gtgctcgcac ccacagcgga caagcccacc gccgccgaga tgctgacgct gtataaccgg
     2701 ggcatggtca agacgctgct ggcccactcg acgcaagtac gcttcaacat gggcagccta
     2761 cccggcgacg tgctccgcag gctctacttc atcgccaagc gcagcggcgt gctcgttgac
     2821 gtggagagcg accgcgcggg cgggttctcc cttaccctgt tcggccctga gcaggcattt
     2881 ggaagcgcgg acaagtacgg gcggcggctg gccgacgtgt cgctgagcct gctgcgctcc
     2941 ctgctcaccc tgcccccttc aacccccgta cagggcaccg cctctctcgt cctccacgac
     3001 cgcacctacc gtttccacct cacccaggac atcctggacc acctggaata tgcacctgag
     3061 cctcaagcgg gcgctcgcac cggccgcgta gccgagagaa gcgccgccta ctcggtaggc
     3121 tcctccctgg accacgaagg ggacgacgct atcgaagacc tggagccgac cttcgacagc
     3181 ctcgtggaag cctcccttta ccgggggttc cgctcgctgg agaagcaggg cgacacccac
     3241 ggctggctca tcctgcggga gcctgacccg ttgctcgcgc ccggcgtggt gctgataccc
     3301 gacttcgcgt tcgagcgcgg cggcacgcgc gtctacatgg aggtagccgg cttctggtcg
     3361 ccctcataca aggagcgcaa ggtcgccaag ctgcacgccc tgggcgcgag cggggaggac
     3421 acggcgctaa tcctggccgt gcccaaggac tccgcccctg ccttcaccgg cctgcccttc
     3481 ccaatcgtgc cctacaagaa ggacatacgc gccaccgaca tgctcgccct gctcgacgcg
     3541 caatacggcg ctcgcgagga gcggctcacg tcggcccgag ggcggttcgc cccgttgcag
     3601 caagccgcac tcaaacgcgg cctggtgccg gagcgcgacc tggccgaggc gttgcaggct
     3661 tactcccgct cggaactgct ggccgtggcc ccggagcttg atacacctgg ctgtcgctac
     3721 gtacccggcg tgggcctcct ctccgacgcc gctgccgacc gtgcccacac cagcctcctg
     3781 gccgcccttg aagcctcgcc tgagcgtcga atcgacctcg accaggcggt agagcttgtt
     3841 tcctccgcgc tcggcgcctc ccacgtagac gtcgaagccc tcctccaact ctggccccaa
     3901 ttccgcgtag accgcccctc cctcttcgaa gccttcatcg ccctcgcgta acggtgacgc
     3961 tccgccgtct ccatgcaccg gcccggtatc ccagtccggc tagaagctgt cgtctcagga
     4021 accaggctgt caaatagcgg cacccaagcc ccaccctagt catttcggac gaagtgagaa
     4081 atctcgtccc ccagtaccca ggtacccccg ttctttcaca accatgctct cttgagaaag
     4141 gagaaaaggg gaacatcctg gttaaggacg agatttctca ctacattcga aatgacaaag
     4201 ccgtcgctat gcacccaaaa tccaggctat tagtgttcaa atggcactcc gtgatacttc
     4261 cttcaaagaa aatcaatatc gttattgaaa aaatgaatgg tcttattaaa ttcatcaatt
     4321 caccccttga caacctgcca aagctatgat attattgggt cagataaaga aaatcacaac
     4381 aaacattgaa gaaattgagt tggaggtaat aaatatgaac ccacttcccg gaagatgccc
     4441 ggtctgcgct aactcactca ctgttacccg cttgcagtgc gggcactgtg gcaccggcat
     4501 cgatggggct ttcggcctcg gccgcttgca ggcgctcacc gccgagcagg tgcagttcgt
     4561 cgagaccttc atgaagtgca agggcaagat caaggacgta gagggagagc ttggcatctc
     4621 ctaccccaca gtggtggccc gcttgaacga agtggtccgc gctatgggct tcgaagtgga
     4681 cgagggcgac ctgagtgacg ttgaccagta cgagtactac caggcccgcg tgctcagccc
     4741 acagatgccc gcccgccccg cgccaccttc tcctatcagc cccatgccgt ctatgcctgt
     4801 ggtcccgccg gt
//
LOCUS       DASRWZ010000596         8261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1076327, whole genome shotgun sequence.
ACCESSION   DASRWZ010000596 DASRWZ010000000
VERSION     DASRWZ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076327"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..1383)
                     /locus_tag="VFH60_09385"
     CDS             complement(265..1383)
                     /locus_tag="VFH60_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314037.1"
                     /translation="MLNRLPCRVAASLGLGLLVSLTAFSTQGLAGASLPPAGKVALQP
                     APTPMPGMPLPGSRSRTFPETGKTVRGIFLDYWEAHGGLPQQGYPVSEAFAEVSDLDG
                     KTYTVQYFERAVFEYHPENRAPTDVLLSQLGTFRYQKKYPNGAPGQQPNTSAGSRLFE
                     QTGKRVGGPFLQYWQQNGGLAQQGYPISDEFQEKSELDGKTYRVQYFERAVFELHPDE
                     KPPYNVLLSQLGTFRLRDKYPNGPPVEPTVPPGPGGSKPAPTSTPTNPACAGIPEGQN
                     MTITPNCGTPRSGFTMRFVSRGFKPGEVVQLTATTPDGLALAVGSRAADAQGVVEINL
                     LIDRVSNWPRGLYTVVAEGDISGKKGTGYFKIIAAGSN"
     gene            complement(1589..1978)
                     /locus_tag="VFH60_09390"
     CDS             complement(1589..1978)
                     /locus_tag="VFH60_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314038.1"
                     /translation="MGTTGRLSAQNGEDPALAARGSPPGRREPLAGAATNTSTNTAMD
                     IDGEDGNGKRSTSALKQRGMKKYQIVVPESLFEEIEKIADSRQTTVIDIIRRFLKLGL
                     LTARIENTANAGLFIREGDSEREIILL"
     gene            2604..6275
                     /locus_tag="VFH60_09395"
     CDS             2604..6275
                     /locus_tag="VFH60_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6314039.1"
                     /translation="MAGNKAIYDTAMKRAHDHAWANQWDRAIKEYDRALAEFPDDLAA
                     CRNRAQCLFRLRQWPEALAAYSSLVQKEPADLFAINRLAEIHLALGQQEEATETYFML
                     AERYYAQHQFHEAIRALRDLARAMPNHREVHVRLLALVREVGDRAAEVVEHVALSRIA
                     LEAGDVATAQREAEIASGLDPESPEVRRWNYTVRRRQAEMNATGSISPDADATGHITT
                     SMGTGLIPQQPDQEPDEALELIGKATEAQNAGDFRLAMELYDQAVRAGAKRASVFYSA
                     GLLNQQMGRPEMAVPLLERAAKDPEFGISASYGLGLAYSAMRNHPQAVAAFEKALGMI
                     AVTSLTRNEADELVELYTAAAEANLADSNPGRASSILNNLVKILKEKKWALPQMADLE
                     RKAEELYSRSIMSKLDGISKGSGMLDAAATAAIGSAELPPMPVMPPHMSMEATAMMVE
                     PPDATNRLVGGATAAMSAQSIADEAMSTQMMQSGALPGTMPIPMPVTDADGGEVKKYS
                     SDIETTMMRQPGSSLRTITEYLRAADLNMDASDEPGERNPFGQHASDAEAAMSDAAPP
                     TTNPEAHTFTNLSTQALLGMEAQSIVVQRAIAEAEFAMHQGQFDSALDSAMSVIEWEP
                     EYLPVHLIMADVYMAQGKTEEATDKLQIVMDVYTSRGDASSAADVCLRLLELQPDNPS
                     LKNRLGVLLMEAGRVDDAADALLSVSDGLYRAGHYEHALEEALALKAQLPESSKVALA
                     VGTYLMTLGRTNEALAELSRALTLDPGNNHALSRVYVALASMGAETEWDALQSLAERA
                     GRDMATNRLFMEELHAAIKWGTVPSVFYGLSVLAERVGLTDIAADALEEGLLQMSLSE
                     TGQIKESWLLLEVLMSQTRADLALNAKDWGVAAQHYNRALGIIKSAGIDEEGRVQIES
                     PRPQYSFVRLAEPAQLYYGLAEAQASQNNWDQAAEALNALKTLMPNDVSVYTRLADIY
                     FRQGNLSQALAELNDLLVTFQRRNDHEKTLETLGHMARLAPNNVPVRKKLADMYIKLG
                     MTDYGLQELNTLAELQLKAGHLKDAMSTYQRAGDMQYTLGRHEEALEIYGRVVRIAPR
                     DIEARQQLINMYVQSGKIQEAIAGERALADLFVQDGYTEEAISALHQLLALSPEDVPA
                     HHKLAQQLMALGRYGEAARLYGRLVRLEPENSRNAIMQSEMLRMAKEAQGDTQPEPDP
                     KSGKGSKQKQMASASSKRG"
     gene            6525..7337
                     /gene="cdaA"
                     /locus_tag="VFH60_09400"
     CDS             6525..7337
                     /gene="cdaA"
                     /locus_tag="VFH60_09400"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET6314040.1"
                     /translation="MQQVSWVLSRINWATIFDVVAVALIFYWLLSVVQGTRAVQLVRG
                     IIILWLAAALLSSVLPLTTLTWLIRNSGLALLVAIPIIFQPELRRALEQLGRTGTWFN
                     RGLFGNSKAIEATTQEVAAACGVLARQKHGALIVIERQTGLQDYVDKAVPLDAEVTRQ
                     LLTTIFYPNTPLHDGAVIIRSNRIVAGHAVLPLSDNVLNATQFGTRHRAALGISEYSD
                     AVAVVVSEERGIISVAANGRLVSNLNEDRLRRVLLDLMRNRANAPRTAEAVT"
     gene            complement(7401..8090)
                     /locus_tag="VFH60_09405"
     CDS             complement(7401..8090)
                     /locus_tag="VFH60_09405"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6314041.1"
                     /translation="MQRNEEFEPVEADRVAGADPVREGLERMALRARAGDEEARNALF
                     LSQKGLVRVLSRQAKRLVRSLERRDQSLQPDDIDQQAFVEFCALLEEWEPRQTPFVAY
                     MRHLLPWRLLHYVRRSVRYRSGVRILPLASFGASNEANGNERGEPGIEDEATRDKIIG
                     IESNDAWRQHTEALDEGLRRAVSLRYGLGFSSREIASMEGRSRRTIDRDLRAAMQKIK
                     RSLQDEWENCS"
BASE COUNT         1599 a         2655 c         2514 g         1493 t
ORIGIN      
        1 tacgttgaag actctggcct acccacctga tccttcgcta cgctcaggat gacaggggag
       61 agtgtggttc ccgtaattgg agcgtgaagg gtgtccacga gggaatagca agcagcaacc
      121 gcataaaaca acccggcacc tccaccgcgc cgcatagtgg tggtgccggg ttgtgggctt
      181 tcgtgccgca ggccaggtct taggacggag ccggggccgc tcgaacagca tcgctgctat
      241 agagggtgcg aaccgggcat cgggttagtt actgcctgcc gcgataatct tgaagtaccc
      301 cgtgcccttc ttacccgaga tgtccccttc cgcaaccact gtgtaaagcc ccctcggcca
      361 gttggagacc cggtctatca ggaggttgat ttcgaccacc ccctgcgcgt ccgctgccct
      421 gctgccaacc gccagggcca ggccgtcggg cgttgtggcg gttagctgca ctacctctcc
      481 cggcttgaag ccccggctga cgaaccgcat cgtgaagccg gagcgagggg tgccgcagtt
      541 gggcgtgatg gtcatgttct gtccctccgg tatgccagcg cacgccgggt tggtgggggt
      601 gctcgtgggg gcgggcttgc tgccgccggg gccgggtggg acggtaggct cgacgggcgg
      661 gccgttgggg tacttgtccc gcaagcggaa ggtgcctagc tgcgacagga gcacgttgta
      721 gggcggcttt tcatcagggt gcagctcgaa caccgccctc tcgaagtact gcaccctgta
      781 cgtcttgcca tctagctcgc ttttctcctg gaactcgtca gagatgggat agccctgctg
      841 ggccagcccg ccgttctgct gccagtactg gaggaagggg ccgcccactc gcttgcctgt
      901 ctgttcgaaa agccgcgagc ctgcggaggt gttgggttgc tgtccgggcg cgccgttggg
      961 gtacttcttc tggtagcgga aagtgcccaa ttgcgacagg agcacgtcag tgggtgcgcg
     1021 gttctcaggg tggtactcaa agacggcgcg ctcgaagtac tgcaccgtgt aggtcttgcc
     1081 gtccaggtcc gacacctcgg cgaacgcttc cgagacgggg tagccctgtt gcggcaagcc
     1141 tccatgcgcc tcccaatagt cgaggaagat gccccgcacc gtcttaccag tctccgggaa
     1201 ggtgcggctg cggctgccgg gtaggggcat gccgggcatg ggggtggggg cgggctgcaa
     1261 agctaccttg ccggccggag gaagtgatgc acctgccagg ccttgtgtgc tgaaggcagt
     1321 aagggagaca agcagcccca acccgaggct ggcggctacc cggcagggta gtctgttcaa
     1381 catgatgacc tccgctcgcg acagatggcc ggcagtacgt gccgggggag cgtcgtggct
     1441 cccgatatat aatccgcgag cagggacgga tagtaacagg catgtgcggg cgcaacaaaa
     1501 gtcccgccgc ccggtggtcc gaacggtggg acttttgttg cgcagccagg ttagccgcca
     1561 ggttagcgaa tgaggaggtg ggccaggtct aaagcaggat gatctctcgc tcgctgtcgc
     1621 cttccctgat gaagagaccg gcgttagcgg tgttctctat tctggcggtg agcaggccga
     1681 gcttgaggaa ccgcctgatg atgtcaataa ccgtggtctg gcggctgtca gcgatcttct
     1741 cgatctcctc gaagagcgat tcgggcacga ctatctggta cttcttcatg ccccgctgct
     1801 tgagcgccga agtcgagcgc ttcccgttcc cgtcttcgcc gtcaatatcc atagccgtgt
     1861 ttgtagaggt atttgtagcc gcgcccgcca acggttctcg cctgccgggg gggctgcctc
     1921 gcgccgctaa tgcggggtcc tcgccgttct gcgccgacaa tctgcctgtc gtacccatgt
     1981 aataccctcc ttgcaaaagg tattgccctc aagcatgaaa atgctatccc cacgcttgct
     2041 gcccagggct tgccgcctca ccggagccaa ccttatggta ttcctatata tcgcaccaca
     2101 gcccgccgct ccgcccgcgg gaggtgccag ggttccacct acaagaagaa tgcctccaat
     2161 taccacgcga ttcctatact atatcgctag aatcgggcag taaataacgc atctgcggca
     2221 atctgcacaa ggtgaagtcc tgtgtaacag tctacaccta ctacaaagct cgcgcaatac
     2281 acgtttatgt cttttttcaa tagcgggtac cgggtaactg ttgccactgc taacaataaa
     2341 tatgcactcc aatccttcca ggatgcatga ataaagaggt agaagggtgg cgaggcagta
     2401 cttccttccc cccacccacg tctctttctc ccatttttag tcctaatcct ctttttattt
     2461 gctatactcc aacagtggga gcatcaggcc agcttgtgcc aggcccccgt tgtttgattt
     2521 tggatttcgg atttgcgagt ccgtccgaag tgaaaaatcc aaaatccaaa gtctaaaatc
     2581 caaaattcca aatcgagggt agtatggcgg gcaacaaggc aatctacgat accgcgatga
     2641 aacgcgccca cgaccatgca tgggccaacc agtgggaccg cgcgattaag gaatatgaca
     2701 gggcgcttgc cgagttcccg gacgacctcg ccgcctgccg caacagggcg cagtgtctct
     2761 tccgcttgcg ccagtggccc gaagccctcg ccgcttacag cagcctggtg cagaaggaac
     2821 cggcggacct cttcgcgatc aaccgcctcg ccgagattca cctggccctg gggcagcagg
     2881 aggaggccac cgagacctat ttcatgctgg ccgagaggta ttacgcccag caccagttcc
     2941 acgaggcgat ccgcgcgttg cgcgacctgg cccgcgctat gcctaaccac cgcgaggtac
     3001 acgtgcgcct gctcgccctc gtccgcgagg tgggcgaccg cgccgccgag gtggtggagc
     3061 atgtcgccct ttcgcgcata gcgttggagg ccggggacgt agccaccgcc cagcgcgaag
     3121 ccgagatcgc ttcgggcctc gaccccgaga gccccgaagt gcgccgctgg aactacaccg
     3181 tgcgccgccg ccaggccgag atgaacgcca ccggcagcat ctcccccgac gcggacgcta
     3241 ccgggcatat taccacctcg atgggcacgg gccttattcc gcagcagccc gaccaggagc
     3301 cggacgaggc gcttgagctt atcggcaagg ccaccgaggc ccagaacgcg ggcgacttcc
     3361 gcctggcgat ggagctttac gaccaggcag tacgggcagg ggccaagcgc gcatcggtat
     3421 tttacagcgc gggcctgctc aaccagcaga tgggccgccc tgagatggcc gtgcccctgc
     3481 tcgaacgcgc cgccaaggac cccgagttcg gcatctcggc cagctacggg ctgggccttg
     3541 cctactccgc gatgcgcaat cacccccagg ccgtggccgc gttcgagaag gccctcggca
     3601 tgatcgccgt gacgagcctc acccgcaacg aggccgacga gcttgtggaa ctgtacacgg
     3661 ccgccgccga ggccaacctg gccgacagca accccggccg cgcctcctcc atcctcaaca
     3721 acctcgtgaa gatcctcaag gagaagaagt gggccttgcc tcagatggcc gaccttgaga
     3781 ggaaggccga ggagctttac agccgctcga ttatgagcaa gctcgacggc atcagcaagg
     3841 gcagcggcat gctcgacgcg gcggccacgg cggcgatagg ttccgccgaa ttgccgccca
     3901 tgcccgttat gccgcctcac atgtcgatgg aggccaccgc catgatggtg gagcctcccg
     3961 acgccaccaa ccgcctcgtg ggtggggcca cagccgccat gtcggcccag tcgattgccg
     4021 acgaggcgat gtctacccag atgatgcagt cgggcgcgct gccgggcact atgcccatac
     4081 ccatgccggt aacggacgcg gatggcggcg aggtcaagaa gtacagcagc gacatcgaga
     4141 ccaccatgat gcgccagccg ggcagcagcc tgcgcaccat caccgagtat ttgcgggccg
     4201 ccgacctcaa tatggacgcg agcgacgagc ctggcgagcg caacccgttc ggccagcacg
     4261 cctccgacgc cgaagccgcg atgagcgacg ccgccccgcc caccaccaac ccggaagccc
     4321 ataccttcac caatctctct acccaggcgc tgttgggcat ggaggcccag agcatcgtgg
     4381 tgcagcgcgc cattgccgag gccgagttcg ccatgcacca ggggcagttc gactccgccc
     4441 tcgactcggc tatgtcggtg atagagtggg agcctgagta cctgccggtc cacctgataa
     4501 tggccgacgt ttacatggcc cagggcaaga ccgaggaggc tactgacaag ctccagatag
     4561 tgatggacgt gtatacgtcc cgtggggatg cgagcagcgc ggctgacgtg tgcctgcgcc
     4621 tgttggagct acagcccgac aacccgtcgc taaagaaccg cctgggcgtg ctgctcatgg
     4681 aagcaggccg cgtggacgac gccgccgacg ccctcctgag cgtgtccgac ggcctctacc
     4741 gcgccgggca ctacgagcac gcattggaag aagccctggc cctcaaggcg caactgccgg
     4801 agtcttccaa ggtggccctg gcagtgggca cgtacttgat gacgctgggc cgcactaacg
     4861 aggcgctggc cgaactcagc cgcgccctca cgctcgaccc cggcaacaat cacgccctgt
     4921 ctcgcgtgta cgtggccctg gcctcgatgg gcgcggagac cgagtgggac gccctgcaaa
     4981 gcctggccga gcgggcgggc agggacatgg cgaccaaccg cctcttcatg gaagagctgc
     5041 acgccgccat caagtggggc accgtgccga gcgtcttcta cggcctgtcg gtgctcgcgg
     5101 aacgtgtcgg cctcacagac attgccgctg acgcgctgga agagggcctg ttgcagatgt
     5161 ccctcagcga gacgggccag atcaaagagt cgtggctgct gctggaagtg ctgatgtcgc
     5221 agacccgcgc cgacctcgcc ctcaacgcga aggactgggg cgtggccgcg cagcactaca
     5281 accgggcgct tggcatcatc aagtcggcgg gcatagacga agaagggcgc gtgcaaatcg
     5341 agtcgccccg gcctcagtac agcttcgtgc gcctggccga gcctgcccag ctttactacg
     5401 ggctggccga agcccaggct tcgcagaaca attgggacca ggccgccgag gcgctcaacg
     5461 cgctgaagac gctgatgccc aacgacgtgt cggtatacac ccgcttagcc gatatttact
     5521 tccggcaggg caacctgtcc caggcgctgg ccgaattgaa cgacctgctg gtgactttcc
     5581 agcggcgcaa cgaccacgag aagacgctgg agacgctggg ccatatggcc cggctcgccc
     5641 ccaacaatgt gccggtgcgc aagaagctgg ccgacatgta catcaagctc ggcatgaccg
     5701 actacggctt gcaggagcta aacaccctgg ccgagctgca actcaaggcg ggccacctga
     5761 aggacgccat gtctacctac cagcgggcgg gcgacatgca gtacacgctg ggtaggcacg
     5821 aggaagcgtt ggagatctac ggccgcgtgg tgcgcatcgc ccctcgcgac atcgaggccc
     5881 gccagcagct aatcaacatg tacgtgcagt cgggcaagat acaggaagcc atcgcgggcg
     5941 agcgcgccct ggccgacctg ttcgtgcagg acggctacac cgaggaagcg atctcggcgt
     6001 tgcaccaact gctggccctc tcgcccgagg acgtgcccgc ccaccacaag ctggcccagc
     6061 aactcatggc tctcggccgc tacggcgagg cagcccgcct ctacggccgc ctggtgcgcc
     6121 tggagcctga gaacagtcgc aacgccatca tgcaaagcga aatgctgcgc atggccaaag
     6181 aagcccaggg cgacacccag cccgaacccg accccaagtc cggcaaaggc tccaagcaga
     6241 agcaaatggc ctctgcctcc agcaaacggg gttaataacg gaggagacgg aaacgcaaag
     6301 acgcaaagaa cgcgcaaagt ttaagattgt tgttctgagt acaaaaagga ccagcgtata
     6361 gaacgacgta cgctggtcct ttttgtactc tgcaaacctt cctaatctct gcgcgttctt
     6421 tgcgtctttg cgtctttgcg tctttgcgtt ttcgtctctt accaaacttg cccgatgcag
     6481 tgtagccgga tagaataggg tatagctggc aaagggggaa gggcgtgcag caggtatcct
     6541 gggtgctgtc gcggataaac tgggcgacca tcttcgatgt ggtggcggtg gcgctcatat
     6601 tctactggct gctgtcggtg gtgcagggca cgcgggcggt gcagttggtg cgcggcatca
     6661 tcatcctgtg gctggccgcc gcgctcctct ccagcgtgct gccactcacc acgctcacct
     6721 ggctgatccg caactccggc ctcgcactcc tggtcgccat ccccatcatc ttccagccgg
     6781 agctacgccg cgccctcgaa cagctaggcc gcacgggtac gtggttcaac aggggactgt
     6841 tcggcaacag caaggcgatt gaagctacga cgcaagaggt ggcggcggca tgcggggtgc
     6901 tcgcgcggca gaagcacggc gcgctcatcg tgatagagcg gcagacgggc ttgcaggact
     6961 acgtggacaa agccgtgccc ctcgacgccg aagtgacccg ccagctactc accaccatct
     7021 tctaccccaa cacccccctg cacgacgggg ccgttatcat ccgcagcaac cgcatagtgg
     7081 ccggtcacgc cgtactgccc ctgagcgaca acgtgttgaa cgccacccag ttcggcaccc
     7141 gccaccgcgc cgccctgggc atctccgagt actcggacgc cgtagccgtg gtggtcagcg
     7201 aggagcgggg catcatctcg gtcgccgcca acggccgcct ggtaagcaac ctcaacgaag
     7261 accgcctccg ccgcgtgctc ctcgacctga tgcgcaaccg agccaacgcc ccacggacgg
     7321 cggaagcggt aacgtaattt ggaatttcgg atttcggatt tcgaatttga ttggatatct
     7381 gctaaattcg aaattccaaa tcaagaacag ttttcccatt catcttgcag gctgcgtttg
     7441 atcttctgca tggcggcgcg taggtcgcgg tcgattgtgc ggcgggagcg gccttccatt
     7501 gacgcgatct cgcggctgga gaagccgagg ccgtagcgga ggcttacggc gcggcgcagc
     7561 ccttcgtcca gcgcctcggt gtgctgccgc caggcgtcgt tgctctcgat gcctattatc
     7621 ttatccctgg tggcttcgtc ttctatgccc ggctcgcccc gttcgttgcc gttcgcttcg
     7681 ttcgatgctc cgaaggaagc aaggggcagt atgcgcacgc cggagcgata gcgcaccgaa
     7741 cgccgcacgt agtggagcaa gcgccagggc agcaagtgac gcatgtaagc cacgaacggc
     7801 gtctgccgag gctcccactc ctccaggagc gcgcagaact cgacaaacgc ttgctggtct
     7861 atgtcgtccg gctgtagcga ctggtccctt ctctccagcg acctcaccaa ccgcttcgcc
     7921 tgccgtgaca gcacccgcac caggcccttc tgcgacagga acagcgcgtt acgggcctcc
     7981 tcgtctcccg cccgcgcccg taaagccata cgctccaacc cctccctcac ggggtcggcc
     8041 ccagccaccc tgtctgcctc gacaggctca aactcctcat ttctctgcat cgccaccgtt
     8101 cctccatgaa aacaaaaata aggaatggct gggttccggc ccaaccattc cttattttag
     8161 aaccggagtt ctattctgga tagtacctat gtgggtacct atgtggacga aggagataag
     8221 tcgtacctgg aagttatctc agagtgccac tgtcgaaaat g
//
LOCUS       DASRWZ010000597         9646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1076464, whole genome shotgun sequence.
ACCESSION   DASRWZ010000597 DASRWZ010000000
VERSION     DASRWZ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076464"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..3830)
                     /locus_tag="VFH60_09410"
     CDS             complement(219..3830)
                     /locus_tag="VFH60_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314042.1"
                     /translation="MRASENPAKRATGEAQRQTVSRSHRARLVLFALLSAFFSFGCIT
                     WTTVVEVTRSEDGKQHSIGTSIRYEFDKGVWAELEADERDSTSDFADPLKEQGWKVET
                     SDGGRIINITRDPAPLDTLKEQGWTVIEQPLETGRIVTATLPFLGAGGTKSDDPAKEP
                     VQNITVVIDESDPSATRYTYRSKVFVDISEQASDSGSSPPPASEDSWGDFIQDAIDQS
                     PDVQRLEEALKNAGPPKLIIGAALPGIIESSTINGEPGGTPGGNSVEWTIDTQKAGTY
                     NLEAKALGAQMELAITQFEIEGTEVTTPDGTAFSISDTLKVNLRVAGQNKDTKDQDFE
                     KFNPIGVKLYYDDKEIDAGQTGEGGNVSFSFTPPADAPAGDAPAGEASEGVSSHTIKV
                     VASKKGFEDAEESKTIEVSSGKKKEGLDLTVDHIEVVQVIQTKDNKIPLVAGKKTVVR
                     VFLKGTGDPSGVISNVRGSIFVWPEGKSEVEVKSAANGSINVINDAEPRRDDTNASLN
                     FVIPRELTGPGVFSIKAVVNPTHGIKESDFDNNELTEAFEFVQRNGLRVGFVRIGYKP
                     PGQTVWAWPGTNIPNYGQMMSKLFPAADGAVQYYELPFRIRQKRLIDSDDAGEELNWS
                     LREFYDRMQGDKPDIMIGWLPNEYASTFPFGGLAETVLSGQVARVALAVDHRTDYSSM
                     HVLPHEVGHNLGLDHTGTRSDSSSDCRLSVNRGSGYWPGEYGDSAAIRDSGFDTSDMR
                     AIPGTFYDLMAYCNEHKTWLSTYHYKKLFDYNLRPQGYFDTTRAHKVWVRGWASLRGD
                     AAFIEFVAPGTDSGGGTSSQALGTSPGRAYGPGLATNFFLPLTAGKMAVAGQVAQREG
                     EGDHCLRFLDAADALQYERCFDLTFQSAETLEPLEGSGFVLGVPDPGNVARVTLVRND
                     GGSEQELTSLEVSANAPTLTITSPKAGDNWAGEHTIEWSGSDRDGDTLRYDIQYSPDA
                     KKSWYPLEVGSHDTAYTFSTDEILPSDQTYIRVTASDGFNTTSADVGPLVIPQQPNSP
                     NPPPAPGVPSTDAPAASASSPLGGLPGWGLMAIIGGGVAALVLVLGMVMFRGRSSRRQ
                     AVAYPSGAGVAPPTYPQARVAPPPPTATAQPYYPPAAPPTQPLQPQPGPTPFHVARLE
                     HARLQGELAAGRINHQQYEAAVNAIRVQDQSGRFWMLGAYDGFWYYYDGRSWVRTNL"
     gene            4667..5140
                     /locus_tag="VFH60_09415"
     CDS             4667..5140
                     /locus_tag="VFH60_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6314043.1"
                     /translation="MTAEIESTGAPVARTGETVEFGGNLMTFLAVGTDTGGAYSVAHY
                     TAQPGSGAPLHLHRNEVESFYILEGAMTFRLGDEKLRATANECVTIPRLTPHAFANAE
                     DIPAKMLVILTPAGLEHYFAELSALFHQPGGPTAEQIASLNEKYNLDFSLGDKTA"
     gene            5396..7645
                     /locus_tag="VFH60_09420"
     CDS             5396..7645
                     /locus_tag="VFH60_09420"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HET6314044.1"
                     /translation="MTTEILLRVFANSFYIYHQRSLLITDRRGRVTQGLEGLYWHDLR
                     LLSRYRLLVNGDEPRLDALSAVDPFSSLAYYVCPANRGDGDKLDHMGLPEHEADRQVV
                     LRVARFCGQGMHEDIEVTNHGTTPAALDLEWELDADFADLIEARSGKRQQEAPIEQAW
                     EGNTLRFTYQHPQLPRGAVMEFEARGSAMRYEGGKARTRLELPPQGTHRFCLNVSPVS
                     DGQTIAPLFGCDAFGSLATETDRQRAAWTLEGTRVYTRNLDVQKTWDRAIQDLGALAA
                     GPTTGEEDELELAVPMAGEPLYGTLFGRDALTLSGQAMFFSPKLAEGALRLLSRYVGT
                     KDDDFYDEQPGRVPQQVRDGPLGLLGITPWLHDYGDYAAPCAFLVLLAGHHIVMGNSE
                     ITREFIEPAKRVLDWLDTRADLDGDGFLEYKTRSSKGQKHQGWKDSGDPVRYEDGREV
                     EPPIAACEIQGYLYAARLLMAEVFLALGEPSRSVDLLRQAQALKKRFNEEFWMPGEKF
                     LALGLDSEKRQIKSIASNAGHCLTTGIIDRKHARDVVRRLMQPDMFSGWGIRTLSSEH
                     PAYNPIKYHLGSVWPVENATFAFGMKRFGFSRECNAVAKGMFDAAALFEHHRLPETIG
                     GFPRDKRHPHPGIYPDACSPQGWSASAIAWLVQAMLGIFTYAPARLLMLDPVLPAWLP
                     DLTLRDLPVGEARVSIRFRRQPDGTTDYKVFERKGAVLVVRQAPPDALGVGIGTRLGQ
                     VVGSVLPWR"
     gene            7733..>9646
                     /locus_tag="VFH60_09425"
     CDS             7733..>9646
                     /locus_tag="VFH60_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET6314045.1"
                     /translation="MYHTSRLSLFTVLAVLVTLIPSYSTLAQGSSRTFPETGKTVKGR
                     FLEYWQQNGGLAQQGFPITDELQEKSDTDGKTYTVQYFERAVFELHPDNQKPYDVLLS
                     LLGAFRYSEKYKSAPQSDSMTLPAGKAPITRCPLTHNDPSPTYRAGMPVRSSVGKGHI
                     VTGYVLSGEGCAPIAGAMLELWPEVGGEHPQEYRATVFTDRDGRYSYESPRPEHIHMR
                     ISAHGYRTIFSNAYHPAEGQAQGTYNVTLAPDPACSLFKETGRSMCGDFLTYWQNNGG
                     LAQQGYPISGQFAETSDLNGQSYTVQYFERAVFELHPENQAPNNVLLSQLGTFRHQVK
                     HGAAGTVKAVSSMSIARSCHSSTLLPNGKVLIAAGMVTEGNYERSAELFDPATNTFAP
                     TGSLAVGRACHNATLLPNGKVLVAGGANGNDSSAELYDPAGGTFTRTGDMGAVRDGAT
                     ATLLKSGKVLLAGGFDFSRRVGQSSAELYDPASGKFTPTGGMRAARSAHTATLLPDGR
                     VLIVGGGIGRNVLSDAEIYDPSRGAFAPAGAMPTPRHKHTAILLAGGQVLITGGSDNR
                     DWNGRYASSLLYDPTRGTFAPASEMSAPRFKLREATATLPSGKVLVAGDSTVVELYDP
                     TSGTFSRVGGSLDT"
BASE COUNT         1798 a         3089 c         2954 g         1805 t
ORIGIN      
        1 cccacagcat cttccttaag tgggaggtta ggtttgatgt cctcgacagc catgtagagt
       61 tggtacttgt gttgctgcgc cggcccacag tagtcagcac ctctgccggt tggcaccctg
      121 aggagccgca cgtcatgccg cagccagatg gagcgagcag gccggctact ggctggcgaa
      181 ggccaccacc cgttctactc cctcatcaac tcgtttcctc aaaggttggt acgcacccag
      241 gagcggccat catagtagta ccagaagccg tcatacgccc ccagcatcca gaagcgacct
      301 gactggtcct gcaccctgat ggcattgact gctgcttcat actgctggtg gttgatgcgt
      361 cccgcggcca gttctccttg caagcgcgcg tgttccagcc gggcgacgtg gaacggcgtt
      421 gggcctggct ggggctgcag gggctgagtt ggaggtgcgg caggtggata gtaaggttga
      481 gcggtggctg tgggtggagg gggcgcgacc cgagcctggg ggtatgttgg cggtgcgaca
      541 cccgccccgc tggggtacgc cactgcctgc cgccgggacg agcgaccgcg gaacatgacc
      601 attcccagca ccaacaccag ggccgccacg ccaccgccta taatcgccat cagtccccac
      661 ccaggtaagc cgccgagggg cgacgaggcg ctcgcggccg gagcatcggt gctcggcacg
      721 ccgggtgctg gcggtgggtt gggcgagtta ggctgttgcg gtatcacaag aggccccacg
      781 tcggcgctgg tggtgttgaa gccatcgctg gccgttacac ggatatacgt ctggtcgctg
      841 ggcagtatct cgtcggtgga gaaagtgtag gccgtatcgt gcgagccaac ctccagaggg
      901 taccacgatt tcttagcgtc ggggctgtac tggatgtcgt agcgcagggt atcgccgtcc
      961 cggtcgctgc cggaccattc tatggtgtgc tcccctgccc agttatcacc ggcctttggc
     1021 gaggtaatcg tcagggtggg tgcgttggcg cttacctcca gggaagtcag ttcctgctct
     1081 gagcctccgt cattgcgcac aagcgttact cgcgcgacgt tgccggggtc gggcaccccc
     1141 aggacgaaac cggagccttc tagtggctct agtgtctctg cggactggaa cgtcaggtcg
     1201 aagcaccgct catattgcaa tgcgtccgcg gcatccaaga atcgcagaca atggtcgccc
     1261 tcgccttcgc gctgcgctac ctggccggct accgccatct tcccggcggt gagggggagg
     1321 aagaagttgg ttgcaaggcc ggggccgtag gcgcggccgg gcgaggtgcc cagggcctgc
     1381 gacgatgtac caccgccgga atcagtgccg ggcgctacga attcgatgaa tgcggcgtcg
     1441 cctctgagtg aggcccagcc tcgcacccag accttgtggg cccgcgtcgt gtcgaaatat
     1501 ccctgcggtc tcaggttgta gtcgaagagt ttcttgtagt ggtaggtcga cagccaggtc
     1561 ttgtgctcat tgcagtacgc cataaggtcg tagaaggtgc ccggtatcgc cctcatatcc
     1621 gatgtatcga agccggagtc ccgtatagct gcgctgtcgc cgtactcccc cggccagtag
     1681 ccggagccac gattgacgga gaggcggcaa tcggagctac tatcactcct ggtgccggta
     1741 tggtccagac ccaggttgtg gcctacttcg tgaggcagca cgtgcatgga agagtaatca
     1801 gtgcgatggt cgacagccag ggcaacgcga gccacctgcc ctgagaggac ggtctccgcc
     1861 agcccaccaa aggggaaggt gctggcatac tcgttcggta gccagccaat cattatgtcg
     1921 ggcttgtcac cctgcatgcg gtcgtagaat tccctgaggc tccaattaag ctcctctccg
     1981 gcgtcgtcgc tgtcaatgag cctcttttgg cgaatcctga atggaagctc gtagtactgg
     2041 acggcaccgt cagcagcggg gaaaagcttg ctcatcatct ggccgtagtt ggggatgttg
     2101 gtgcccggcc acgcccacac tgtctggccc gggggcttgt accctatgcg gacgaaacct
     2161 acccgcaggc cgttgcgctg gacgaactcg aacgcctcgg tcagctcgtt gttgtcgaaa
     2221 tctgattcct ttattccgtg ggtggggttc acaactgctt ttatgctgaa aacgcccggc
     2281 ccggttagct cccgtggaat gacgaagttg agcgaggcat tggtgtcgtc acgtcgaggc
     2341 tctgcgtcat ttatgacgtt tatagagcca ttcgccgcag atttgacctc cacttcgctc
     2401 ttaccttcag gccagacaaa gatcgagcct ctcacgttac tgatgacccc agatgggtcg
     2461 ccggtgcctt tcaggaagac gcgcaccacc gtcttcttgc ccgccaccaa cggtatcttg
     2521 ttatctttgg tctggattac ctgcaccacc tcgatatggt ccacggtgag gtctaagcct
     2581 tcctttttct tgccgctact gacctctatc gtctttgact cctcggcatc ctcaaagccc
     2641 ttcttgctgg ctacgacctt gatggtgtgg ctgcttaccc cttcagaagc ttcacccgcc
     2701 ggagcgtcac ccgcgggagc atcagccggt ggagtgaagg agaacgagac gtttccgccc
     2761 tcgccggtct ggccggcatc aatctcctta tcatcgtagt agagcttgac gccgataggg
     2821 ttgaacttct cgaagtcctg gtctttggtg tctttgttct gcccggcgac gcgcaggttc
     2881 accttcaatg tgtcgctgat ggaaaaggca gtaccatcgg gcgtggtaac ttcggtgccc
     2941 tcaatctcaa actgggtgat agccagttcc atctgcgcgc cgagcgcttt agcttcgagg
     3001 ttgtaagtcc ctgccttttg ggtgtcgatg gtccactcta cgctgttccc gccgggcgtg
     3061 ccgcccggct cgccgttgat ggtggagctc tcgatgatgc ccggcaacgc agcgccgatg
     3121 attagcttgg gtgggccggc gtttttcagc gcttcttcga gcctctgcac atcggggctc
     3181 tgatctatag cgtcctggat aaagtctccc cagctatcct cagaggctgg cggtgggctg
     3241 gagccggagt cggaagcctg ctcagatata tctacgaaaa ctttagagcg gtacgtatac
     3301 ctggtcgctg agggatcgga ttcatcgatc acaacggtga tgttctggac cggctcttta
     3361 gcgggatcgt cagatttagt gccccccgcg ccgaggaagg gcagggtggc cgtgacgatg
     3421 cgcccggtct ccaaaggctg ctcgatgacg gtccagcctt gttccttgag cgtgtcaagg
     3481 ggagcggggt ctcgcgtgat attgatgata cgcccgccat cgctggtctc caccttccag
     3541 ccctgctcct tcagggggtc ggcaaagtcg gacgtgctat cgcgctcgtc ggcctcgagc
     3601 tcagcccaca cgcccttgtc gaattcgtag cgtatgctgg tgccgatgga atgctgcttg
     3661 ccatcctcgc tgcgcgtgac ctctaccact gtggtccagg ttatgcagcc aaagctgaag
     3721 aacgcactca atagagcgaa caacacgagt cgcgcccggt gcgaccggga caccgtctgc
     3781 ctttgcgcct cgccggttgc acgctttgct ggattctcag atgcacgcat agtcttgttc
     3841 tccctttcta atcgacaagg gccggctcgc atccatgacc tgccatcctg ggtacttccg
     3901 tgggaactaa gccccttcag cccgaacagc gtcacgattc caagcaaacc cacagccata
     3961 agcatggcct ggtggagggc gtgttcgttc gtttcattgt agctccctgg cgcgcaggcc
     4021 aagcagggat accaggaatt gtgggaccta tcgagaggta acttacacct gcgtccgcta
     4081 gggtagtcaa gggggtgcaa ctgaggagcg gagagggcac gcgcaggttt gcggcttctt
     4141 attgagtgag gtaagaagaa cggcaacgca gcaacagact cgccaaagca gcctgctaca
     4201 ataagtatca tatcaccact gtcaagcctc cttccgagga atacaccccg gccacagtga
     4261 aaatagccgc cataagtccc ccctggtatt gtcgcgactg gcagtccagt gccaggtatg
     4321 atgaaattgg ccactacgcc gggcgaaatt gcctagaaaa ttgccctggc aacctattga
     4381 caagctttgt agtttctgct agtattatcg ggcaaagcga tttctcgcga tgcaaggacc
     4441 tcagaggatc ttttcctaga tcgtctgagg tttctttttg ccctccataa ggcaaataca
     4501 acgccgattt ttgcgttagc tgccgcgcat tacctgcgat taataagcag cgggccggaa
     4561 gcgctatgat gtaccgctcg ggcgcttccc ggccctgcga ccagacttcc ccggccacac
     4621 gggccattgc cggctcgaac agcaggcaag aaagagagac aagcccttga ccgccgagat
     4681 cgaaagcacg ggcgcacccg tcgcgcgcac cggggagacc gtggagttcg ggggcaacct
     4741 gatgaccttc ctcgccgtgg gcacggacac ggggggtgct tactctgtgg cccactacac
     4801 ggcacagcca ggctccggcg cgcccctgca ccttcaccgt aacgaggtgg agtcgttcta
     4861 catactggaa ggcgcgatga ccttccggct gggcgatgag aagctacgcg ccaccgccaa
     4921 cgagtgcgtc accatcccca gactcacgcc ccacgcgttc gcaaacgcag aggacatccc
     4981 cgcgaaaatg ctcgtcatcc tcacccccgc tggcctggag cactacttcg ccgagctgtc
     5041 ggccctgttc caccagccgg gcggcccaac cgccgagcag atcgcctctc tcaacgagaa
     5101 gtacaacctc gatttcagcc tgggcgataa gacggcttag cgcaccagga agcagagcac
     5161 gcagtcggag cgcgcagtcg ccgggatcag gggaagggcc gaattcgaga gtagaatgat
     5221 agtgcccggc ctgtgacttg tgccggtgcg ccaattcgta taacctatgt agggtaggcg
     5281 tggagcctgc cctttttgtt cgccccggcc acggctgtta ccctatagcg tcccatactt
     5341 aaaggcagcc accaacgtcc cttgccccaa accctacacg atccaggaag ctcgcatgac
     5401 caccgaaatc ctgctacgcg tctttgccaa tagcttctac atttaccacc aacgctcgct
     5461 cctgataacg gaccgccggg gccgcgtcac tcagggcctc gaggggctgt actggcacga
     5521 cctgcggctc ctgtcgcgct acaggctcct cgtcaacggc gacgaaccac ggctcgacgc
     5581 gctgtcggcg gtggaccctt tttcctcgct cgcctactac gtgtgccccg ccaaccgggg
     5641 cgacggcgac aagctcgacc acatgggcct gccggagcac gaagccgaca ggcaggtagt
     5701 gctcagggtg gcgcgcttct gcggccaggg catgcacgag gacatcgagg ttaccaacca
     5761 tggcaccacc cccgccgcgc tcgacctgga atgggaactc gacgccgatt tcgcggacct
     5821 gatagaggca cggtccggta agcgccagca ggaggccccc atagagcagg cgtgggaggg
     5881 taacacccta cgcttcacct accaacaccc gcaactgccc aggggcgccg tgatggagtt
     5941 cgaggcgcgg ggctccgcga tgcgctatga aggcggcaag gcgcggacgc ggttggaact
     6001 gcccccacaa ggtacccatc gcttctgcct gaacgtctcg cccgtgagcg acggacagac
     6061 tattgctccg ctgttcggct gcgatgcgtt cggctccctc gccacagaga ccgaccggca
     6121 gcgggccgcc tggaccctcg aaggcacccg cgtctacacc cgcaacctcg acgtgcagaa
     6181 gacctgggac cgcgccatcc aggacctggg tgccctggcg gcaggcccga ctacggggga
     6241 agaggacgag ctggagctgg cggtgccgat ggcgggagag ccgctgtacg gcacgctgtt
     6301 cggccgggac gcgcttaccc tgagcgggca ggcgatgttc ttttcgccga agctggccga
     6361 gggggcgttg cggttgctgt cgcgctacgt cggtacgaag gacgacgact tctacgacga
     6421 gcagccgggc cgcgtccccc agcaggtgcg cgacgggcct ctgggcctgc tcggtatcac
     6481 cccctggctc cacgactacg gcgactatgc ggccccctgc gcgttcctgg tgctactcgc
     6541 cgggcaccac atcgtcatgg gcaatagcga gataacgcgc gagttcatcg agcctgccaa
     6601 gcgggtgctc gactggctcg acacgcgggc cgacctcgac ggcgacggct tccttgagta
     6661 caagacccgc tcctcgaagg gacagaagca tcagggctgg aaagactccg gcgacccggt
     6721 ccgctacgag gacggccgcg aggtggagcc tcctatcgcg gcctgcgaga tacagggcta
     6781 cctgtacgcc gccaggctgc tcatggccga ggtgttcctg gcgctgggcg agccttcccg
     6841 cagcgtagac ctcttgcgcc aggcccaggc gctgaagaag cgcttcaacg aagagttctg
     6901 gatgccgggt gagaagttcc tggcgttggg cctcgactcc gagaagcggc agataaagag
     6961 catcgcttcc aacgccggac actgcctgac cacgggcatc atcgaccgca agcacgcccg
     7021 cgacgtggtg cggcggctga tgcaacccga tatgttctcc ggctggggca tacgtacgct
     7081 gtcgtcggag cacccggcct acaaccctat caagtaccat ctcggctcgg tgtggccggt
     7141 ggagaacgcc accttcgcgt tcggcatgaa gcggttcggg ttctcccgcg agtgcaacgc
     7201 agtcgcaaag ggcatgttcg acgccgcggc cctgttcgag caccaccgcc tgccggagac
     7261 gataggcggc ttcccgcgcg acaagcgcca cccccacccc ggcatctacc cggatgcgtg
     7321 ctcccctcaa ggctggtccg cgagcgccat agcctggctc gtgcaggcga tgctgggcat
     7381 ctttacgtac gcgcccgccc ggctgctcat gctcgacccg gtgctgcccg cctggctgcc
     7441 ggacctcact ctgcgcgacc tgccggtagg cgaagcacgc gtctccatcc gcttccgccg
     7501 ccagcccgac ggcactaccg actacaaggt gttcgagcgc aagggcgcgg tgctggtcgt
     7561 gcgccaggcc ccacccgacg ctctgggcgt gggcattggc acgcgcctgg gccaggtcgt
     7621 aggctcggtg ctgccctggc gctagagcga tagcggccag tctcgcgggg ttctgggaac
     7681 caatatggta gcagcggggt actcttacca aataccccac acaggagcta ccatgtatca
     7741 cacctcgcgc ctatctctct tcaccgtgct tgcggtgctc gtcacgctta taccttcgta
     7801 ctctaccctc gcacaaggta gctcccgaac tttcccggag acaggcaaga cggtgaaagg
     7861 gcggttcctg gagtactggc agcagaacgg cgggcttgct cagcagggct tccccatcac
     7921 ggacgagttg caggagaaaa gcgacaccga cggcaagact tacaccgtgc agtacttcga
     7981 gcgcgcggtg ttcgagctac accccgacaa ccagaagcct tacgacgtgc tcctctccct
     8041 cctgggtgcg ttccggtact cggagaaata caagagcgct ccccagagcg acagtatgac
     8101 ccttcccgcg ggcaaggcgc ccattacccg ctgtcccctc acccacaacg acccctcgcc
     8161 cacctaccga gcgggtatgc cggtgcgctc gtcggtcggt aagggtcata tcgtgaccgg
     8221 ctacgtgttg tcgggcgagg gctgcgcgcc aatcgcggga gctatgctcg aactgtggcc
     8281 cgaggtgggc ggcgagcacc cgcaggaata ccgggccacc gtctttacgg accgggacgg
     8341 caggtatagc tacgaaagcc cccggcccga gcacatacac atgcgaatat cggcgcatgg
     8401 ctacaggaca atcttttcca acgcctacca ccctgccgag ggccaggcgc agggcaccta
     8461 caacgtgacg ctcgcccccg accccgcttg cagcctgttc aaagagaccg ggcgctcgat
     8521 gtgtggcgac ttcctcacgt actggcagaa caacggcggc ctggcacagc agggctaccc
     8581 aatcagcggg cagttcgcgg aaaccagcga cctcaacggc cagagctaca ccgtgcagta
     8641 cttcgagcgc gcggtgttcg agttgcaccc tgagaaccag gctcccaaca acgtgctcct
     8701 ctcccagcta gggacgttcc ggcaccaggt caagcacggg gcggcaggca cggtaaaggc
     8761 agtaagcagc atgtccatcg ccaggtcgtg ccactcgtcc acgctgctgc ctaacggcaa
     8821 ggtgctcatc gcggcgggga tggtgaccga ggggaattac gaacggagcg cggaactgtt
     8881 cgacccggcg accaatacct tcgcgcccac ggggagcctg gcggtggggc gcgcctgcca
     8941 taacgccacg ctgctgccca acggtaaagt actagtagcg ggcggggcga acggcaacga
     9001 ctcgagtgcg gaactgtacg acccggccgg cggcaccttc acccgcaccg gggacatggg
     9061 cgcggtccgc gatggggcca cggctaccct gctcaaaagc ggtaaggtgc tcctcgccgg
     9121 gggattcgac ttcagcaggc gcgtggggca atcgagcgcc gagttgtacg acccggccag
     9181 cggcaaattc acccccacgg gcggcatgag agctgcccgc agcgcccaca ccgcgacgct
     9241 actacccgat ggcagggtgc tcattgtggg cgggggcatc ggcagaaacg tgctctcgga
     9301 cgccgagata tacgacccat cgaggggagc gttcgcaccc gccggtgcca tgcccacccc
     9361 caggcacaaa cacacggcca tactgctggc cggcggtcag gtgctcatca cgggcggctc
     9421 ggacaaccgc gactggaatg ggcgctacgc cagcagcctg ctctacgatc ccacccgcgg
     9481 cacgttcgcc cccgcaagcg agatgagcgc gccccggttc aagctacgcg aagccaccgc
     9541 cacgcttccc agcggcaaag tgctcgtagc cggtgactcc accgtggttg agctttacga
     9601 ccccacatcc ggcacgttca gccgcgtagg aggctccctc gacacc
//
LOCUS       DASRWZ010000598         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_322689, whole genome shotgun sequence.
ACCESSION   DASRWZ010000598 DASRWZ010000000
VERSION     DASRWZ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322689"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFH60_09430"
     CDS             complement(<1..248)
                     /locus_tag="VFH60_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET6314046.1"
                     /translation="MSRARTNLTADTAPGSKPLLRGWSHAAAAAGSLLLTVALCWASR
                     DDPPKMVAMLVFGLSMLEMYTVSAIYHMGRWRDSQLRVL"
     gene            complement(373..>3458)
                     /locus_tag="VFH60_09435"
     CDS             complement(373..>3458)
                     /locus_tag="VFH60_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314047.1"
                     /translation="LSSRRVLVTAMFSLALLLSMGLSVRGSYAADTVLQTRPQDQVTQ
                     SAGKGAEKGSQGVPPAFGGLLGNDAVAGAEAAEGGDKVARPEAPPCSPDWTLVASQNR
                     VTRESQILGISASSANDVWAVGYYGDGPTGSVRTLIERWNGTEWLRVPSYNEGTGGNI
                     LRGVVAVSPTDAWAVGSYYDTGTGKWHSLILRWNGTNWYKLSTIIPTAHSDELFSISK
                     GEGDSIWAVGYYEPSAGLRQTLAMRWTGATWNLSYPPNPGGVTENHTLTSVSIVPGSG
                     GADIWAAGYYDGGTYDQTLVLRWTGSSWAHVPSPSPGPSANNRLMGVSASGTNDVWFS
                     GYYGPLGSYVPITLRWTGSGFTTPPTPSAGTWAQLFQIVALAPNNVWTVGYYGSGSLP
                     RTLVEHWDGAAWSIVPSAAPGYASGLQAVTAIDANTVWAAYVYVPEMPDPPHTLIERF
                     DGSSFVQVPSPNGPTDANVLLGVSARTPTDVWAVGASGSDLFSASTLVQHWDGAAWTI
                     VRSVSIGSMSVLQDVAAVAQNDAWAVGFSGSGPQRTLIERYNGTEWSPVTSPNVGAND
                     NNLLGVAASSANDVWAVGSYDDGTAKRTLVLRWNGTAWNTVTSPNPAGSSVDNILESV
                     AVVPGTMGTRAWAVGHTDGFGVTPEAILMGWNGSAWEAVTLSLPAGSTELVDVSARAH
                     NDVWAVGSTDPGSGRRTLVLHYDGTTWSQWTSPNQGAGDNELESVVAIAPNDVWAAGK
                     YQPGPDSEKSLLMHYDGSAWSIVPSPSPGHRDRVLDLAAVSSYNVWGVGSTQASDVSS
                     PIWGMQSLVMQLNECPCTIQFNDAPTGSPFYENVRCLACRDILSGYGCGAPDEPCPGS
                     YFRPNANVTRGQASKIIANAAGYNDPIPATRQTFRDVPPDSPFWLFIERVYAHGAISG
                     YACGAAGEPCPGAYFRPGANLTRGQLAKISTEVAEYNETPSGQSFADVPPDSPFDVYI
                     ERARLHDVISGYACGAAGEPCPGANFRPGNNVTRGQTAKIISNTFYPACVTPARP"
BASE COUNT          576 a         1084 c         1127 g          671 t
ORIGIN      
        1 agcacgcgca gttggctgtc gcgccaccgg cccatgtggt agatggcgct gaccgtgtac
       61 atctcgagca tgctgaggcc gaacaccagc atggctacca tcttcggcgg gtcgtcgcgg
      121 ctggcccagc acagcgccac ggtgagcagc aacgaaccgg cagccgccgc cgcgtgcgac
      181 cacccgcgca ggagcggttt ggaccccggc gcggtatcgg ctgtgaggtt ggtccttgcc
      241 cggctcatga ttgtatttta cctttacccc ggtgataggt cctgcttatt gtagcaagaa
      301 aatagaacaa tcaagcaaaa gctctacctg gttacacagc agagagcttc agcttcgagt
      361 tgcgaggggt tgctacggac gggcgggggt cacacaggcg ggatagaagg tgttggatat
      421 tatcttagct gtctgccctc tcgtcacgtt gttgccgggc cggaagttgg caccgggaca
      481 cggctcgcct gcggccccgc acgcatagcc ggagatgacg tcgtgcaggc gcgcccgctc
      541 gatgtaaacg tcgaaggggc tgtcgggagg cacgtccgcg aacgactggc cgctcggcgt
      601 ttcgttgtac tcggctacct ccgtcgagat cttggctagc tggcccctgg tgagatttgc
      661 gccaggccgg aagtaggcac cgggacacgg ctcgcccgcg gccccgcacg cgtagccgct
      721 gatcgccccg tgcgcgtaga cgcgctcgat gaagagccag aacgggctgt ccggcggcac
      781 gtcgcggaag gtctgccgcg tggcgggtat agggtcgttg tagcctgcgg cgttggcgat
      841 aatctttgag gcttgccccc tggtgacgtt ggcgttaggg cggaagtagg agcctgggca
      901 aggctcgtcc ggggcaccgc acccgtagcc ggagagtatg tcgcggcagg ccaggcagcg
      961 cacgttttcg tagaagggcg agccggttgg cgcgtcgttg aactggatag tgcaggggca
     1021 ctcgttgagc tgcatcacta gcgattgcat gccccagatc gggctgctga cgtcagacgc
     1081 ctgggtgctg cccactcccc acacgttgta ggatgatacc gcggccaggt ccaggactct
     1141 gtcgcggtgg ccggggcttg ggctgggcac aatgctccag gcgctgccgt cgtagtgcat
     1201 gaggagcgat ttttccgagt cggggccagg ctggtacttg cccgccgccc atacgtcgtt
     1261 gggggcgatg gctaccacgc tttccagctc gttgtcgccc gcgccctggt tggggctggt
     1321 ccactggctc caggtcgtgc cgtcatagtg caggacgagg gtgcgccgcc cgctaccggg
     1381 gtccgtgctg cccaccgccc acacgtcgtt atgggcgcgg gccgatacgt caactaactc
     1441 cgtgctcccg gcgggcagcg aaagggttac tgcttcccag gcggacccat tccagcccat
     1501 caagatggct tccggcgtga caccaaagcc gtccgtgtgg ccgaccgccc aggcccgcgt
     1561 gcccatagtt ccgggcacca ccgccacgct ttccaggatg ttgtctacgg acgacccggc
     1621 agggttggga ctagtaaccg tattccaagc ggtaccgttc cagcgcagga caagcgtcct
     1681 cttggctgtg ccatcgtcgt agctacccac ggcccacacg tcgtttgccg aggaggcggc
     1741 aacccccagc agattattgt cgtttgcccc gacgttgggg ctggtcacgg gggaccactc
     1801 ggtgccgttg tatcgctcga ttagagtacg ttgcgggccg gagccggaga agcccaccgc
     1861 ccacgcgtcg ttctgcgcaa ccgccgccac gtcctgtagc acgctcatgg agccaatgct
     1921 tacgcttctc acgatggtcc acgctgcgcc gtcccagtgc tgtaccaggg tgcttgctga
     1981 gaagaggtca ctgcccgacg ctcctaccgc ccacacgtcg gttggcgtgc gcgccgatac
     2041 tccgaggagt acgttagcgt ctgttggacc gttggggctg ggcacctgta cgaagctgct
     2101 gccgtcgaaa cgctctatga gcgtgtgcgg ggggtccggc atctcgggta cgtaaacata
     2161 cgccgcccac acggtgttgg cgtctatggc cgttaccgct tgcagcccgg aggcatatcc
     2221 gggggcggcg cttggcacta tgctccatgc agcgccgtcc cagtgctcca cgagcgttct
     2281 gggcaggctc ccgctaccgt agtagccgac ggtccacacg ttattgggcg cgagggccac
     2341 aatctggaag agttgcgccc aggtcccggc gctcggtgtg ggcggtgtgg tgaaaccgct
     2401 gcccgtccag cgcaaggtaa tgggtacgta tgagccgagt gggccgtagt aacccgaaaa
     2461 ccatacatcg ttggtacctg atgccgacac tcccatgagg cgattgttgg cggaagggcc
     2521 ggggctgggg ctgggtacgt gtgcccagga cgagccggtc cagcgcagca cgagggtttg
     2581 gtcgtacgtg ccgccatcat agtagcctgc cgcccagatg tcggccccgc cacttcccgg
     2641 cactatgctg accgaggtga gtgtgtgatt ttcggttacc ccgcccggat tgggaggata
     2701 gctaaggttc cacgttgcgc ccgtccagcg catcgccagg gtctgccgga ggccggcgga
     2761 cggctcgtag tagcccacgg cccatatgct atcgccctcg cctttggaaa tcgagaacaa
     2821 ttcatcgctg tgcgcggtgg gaatgatggt gcttagctta taccagttgg tgccgttcca
     2881 ccgcaggatg agcgagtgcc acttgccggt gcccgtatcg tagtaggagc ctaccgccca
     2941 ggcatcggtg ggtgaaaccg cgacgacgcc gcgcaggata ttaccgcccg taccttcatt
     3001 atagctgggg accctcagcc actcggtgcc gttccagcgc tctatgaggg tcctgacgct
     3061 gccggtcggg ccgtctccgt aataaccgac cgcccatacg tcgttggctg acgaggcgga
     3121 gatgcctaat atctggctct ctcgcgtaac cctgttctgg ctggcgacca gcgtccaatc
     3181 agggctgcag ggtggtgcct ccggtctggc aactttatcc ccaccttcag cagcctccgc
     3241 gcctgcaaca gcgtcattgc cgagcaggcc accgaacgcc gggggcacgc cctggctacc
     3301 tttttcggca cccttacccg ctgactgggt gacctggtcc tgtggtctgg tctgaagcac
     3361 ggtatctgcc gcgtagcttc cacgcaccga cagacccatc gacagcagca aagccaggct
     3421 aaacatagcc gttaccagga ctctacggct gcttaggt
//
LOCUS       DASRWZ010000599         7100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1007786, whole genome shotgun sequence.
ACCESSION   DASRWZ010000599 DASRWZ010000000
VERSION     DASRWZ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007786"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..2580)
                     /locus_tag="VFH60_09440"
     CDS             complement(352..2580)
                     /locus_tag="VFH60_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314048.1"
                     /translation="MSLTSGVRAAIASASLGRHLVALVAVGAVVLGMAYLAFRVSADE
                     TVRIGTEADRIYTAAGFHDPEKSAEQGIYRWTEPDARFVLPNWGPGRVHVTLEGVEGG
                     PGPRTLTLSAGGSDLALVETQPGQPWSLQAWVPAASRAPTVTLSAPQLVVPGDNRVLG
                     TLVKEVSIYAPDARLRAWVDLALLGVAAVLLYACMLLWFGRYLLALACGVALPAVFGP
                     LTAYRDPWMDTVAWTAPLVLGGLLLLGGRGGPGRASPRRRTVYAVGALSAALLLLTMG
                     FLNAFDSDRMYQVVGGFAEYGRPSRYPSLDAFTKYGFGLPLVAVPFYWLGKLGTLFGA
                     AYEPVTRFAVSMTNLPLLAVTCWVLYRASRRFATVGVALAVAGTYLLATPALNYARTF
                     FSEPVGALLLLGSTLLLVPRAGERAPTQRRVLVAGMLLGGMVWLKPAFAVFLPLPGVA
                     VLLMAALHARTEGAARGEMLRRVFVAGAVFSVGPIIAALGQVLYNWLRFAPLADAWLR
                     TGYEREPGFSTPLLVGLEGLLLSPGKSMFLYAPPLLLAPLGMWLMWRRGAYPGRVAVA
                     LIAAHTFMGLVFNATWWAWTGNFAWGPRLIMPLFPLLAWPLASVGEFALAKGRTLLSP
                     RSALLGAWGVLAVLGAVVSIPGALVDFQVYYRLHGLILAGDPGEEFTIYDPAQSPLLE
                     EPGYLLDGLTAAIHRPSLVDVGLPGVWDVLVPGGLVLGAVGLLWLARGRDVGGEGVVG
                     SG"
     gene            2724..3158
                     /locus_tag="VFH60_09445"
     CDS             2724..3158
                     /locus_tag="VFH60_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6314049.1"
                     /translation="MGKIITELAVADMERSLQFYTALGFVKDNEGIVDDNGAQWYSLA
                     LGEGSLWLIRADVTDGLIEADPRGNGVTIYIAVDDIDTLHERVSDAGLLMNIVKPIET
                     MWYGLRQFSLADPDGYILTLNQPIEQADSGEGESGEGQASGE"
     gene            3151..3870
                     /locus_tag="VFH60_09450"
     CDS             3151..3870
                     /locus_tag="VFH60_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET6314050.1"
                     /translation="MSSPTGEKQPTPGDKPPRPSAVQVAPYLQAILDHLSAADPVMAR
                     LVEERGPLSITASPQDDYFFTVLDAIASQQLSSKVAATIVSRIRALVPGEDTPTAAQI
                     FALPDQALRDVGLSWAKVSYIKDLARRVVSGELDLVHVSAMSDEEIIKELVAVKGIGR
                     WTAEMFLLFSLARSDIFAVDDYGLRVAVKRLYDLPDLPKPGPMREIAEPWRPYRSYAS
                     LYLWRSLDNSPKVEAMEVKGS"
     gene            complement(4240..6615)
                     /locus_tag="VFH60_09455"
     CDS             complement(4240..6615)
                     /locus_tag="VFH60_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314051.1"
                     /translation="MVAGARARQPLLPANRLYFAPTGHNIEGKFLAYWQAHGGVPVFG
                     YPLTEAFYEGGYLVQYFERSRFELHPENAGSAYEVLLGQLGAERLKAEGRKVSPTSAP
                     PGPGELTFPETGYRVGRAFLAYWQGHGRLAQFGYPLSPELKDEASGLTVQYFERARFE
                     WHPEHAGTPYEVLLTLLGLERAASLDPTLRDPWPDVPSPNLRLGGTGSELPLLSAVEV
                     ESEVAGEVRVLGGDNRLYATYPLEAGKALSVTLAGAPGRQSFALYREGRIVAALWGAV
                     RVAPPELGIVTGDVVWDGLYQRVQGYLAQDVVEYTSPDNTTPVRGYRSPDDASIWLRD
                     HVHQLKGARYFEPDMTSALEHFRRAQRADGSFDDYYLHTPATPVYSGQIEIEADREYL
                     FVEGVWLAWQATGDDAWLRDMIPSMERGLEHTFNDPRRWSAEHGLVKRAFTIDTWDYE
                     QGSDFGTVRRSIDEHTRWSLMHGDNTGTYRAARLLAGIERYFGRNTEAAKWDGRAEAL
                     RLDLNRVAWNGHFYTHQVHLTPVDPSGVDESTQLSLSNAYALTRGTLSHEQAVEVLRT
                     YQARRIANAGRSFAEWYSIDPPFPRTFGPPGDYLNGGIMPLVGGELARGAFEHGFEAY
                     GADILRRYWDLTERTGGSYLWYHPEGHPGVSNPQTLATDGWGSAAMLNALTEGLAGVV
                     DDGKLYEAVALSPRWAVTGQKSARVALPYGASGAYFAYNWQLHPDGSITLAWGGRQTK
                     SVHLHMLLPGDKAPRQVLVGGAPVAFTTSMVEGSLYLDVALPGAGSLEVQY"
     gene            complement(6720..6914)
                     /locus_tag="VFH60_09460"
                     /pseudo
     CDS             complement(6720..6914)
                     /locus_tag="VFH60_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
BASE COUNT         1428 a         2458 c         2137 g         1077 t
ORIGIN      
        1 gttcaagcgg cctaaagtat cagtcttgct gagcaacaaa acagatagga acagggtatt
       61 ggtcgccgag agagtgaact gactaacggc cctaattgct gggtgtccct cacagagatt
      121 agaggcctcc gactgcatat agagaaccaa catacagtcc aaacatcggg ctatatttac
      181 cacgccaata ccgagaagct acttcagcta gtagggtccg tagccgtagg tgggaagcat
      241 aaccaccgca ccaacattgt tgccacaatg caagcaaacg gcatcacaca tttactcact
      301 gagattgtct cagatttcgc ccgttactca cagctaatca cagtcgttcc cctaacctga
      361 cccaacgacc ccctccccac ccacatcccg ccccctcgcc agccacaaca accccaccgc
      421 cccaagcacc agccccccag gcaccagcac atcccacacc ccaggcaaac ccacgtccac
      481 caggctgggc ctgtgtatcg ccgccgtcag cccgtccagc agatagcccg gctcctccag
      541 cagcggcgat tgcgcggggt cgtagatggt gaactcctcg ccagggtccc ccgccaggat
      601 gaggccgtgc agccggtagt acacctggaa gtccaccaac gcgcccggta tcgacaccac
      661 agcacccaac acagccagca ccccccacgc gcccaacagg gcagaccggg gcgatagcag
      721 cgttctgcct ttagccagtg cgaactcacc caccgaggcg agcggccacg ccagcagcgg
      781 aaatagcggc atgatgaggc gcggccccca ggcgaaattg cccgtccacg cccaccaggt
      841 ggcgttgaag accaggccca tgaaggtgtg cgccgcgatg agggccaccg ccaccctgcc
      901 ggggtaggcc ccacgccgcc acatgagcca catgccgagg ggcgcgagca gcaggggcgg
      961 cgcgtacagg aacatgctct tgccggggct gagcagcagg ccctcaagcc ccaccagcag
     1021 cggcgtgctg aagcccggct ccctctcata cccggtgcgc agccaggcgt cggccagggg
     1081 cgcgaaacgc agccagttgt aaagcacctg ccccagcgcg gcgatgattg gccccaccga
     1141 gaagacagcg cccgccacaa acacccgccg cagcatctcg ccacgcgctg caccttcggt
     1201 gcgggcgtgc agggcggcca tgagcaacac cgccaccccc ggcaggggca ggaacaccgc
     1261 gaacgcaggc ttgagccaca ccatcccccc cagcagcatg cccgcgacca gcacccgccg
     1321 ctgagtgggg gcgcgctccc ccgcacgtgg caccagcagc agcgtggagc caagcagtaa
     1381 cagcgcgccc accggctcgg agaagaaagt acgagcgtaa ttgagggcgg gggtcgcgag
     1441 caggtaagtc ccggctaccg ccagcgcaac gcctacagtc gcgaaacgcc gggaggcgcg
     1501 gtacagcacc cagcaggtga cggccagcaa gggcaggttg gtcatcgaca cggcgaagcg
     1561 ggtgacaggc tcgtaggccg ccccgaacag ggtgcccagc ttgccgagcc agtagaaagg
     1621 caccgccacg agcggcagac caaagccgta cttggtgaaa gcgtcgaggc ttgggtagcg
     1681 cgagggccgc ccgtactccg cgaagccacc caccacctgg tacatcctgt ccgagtcgaa
     1741 agcgttgagg aagcccatcg tcagcaggag cagcgcggcg gagagggccc ccactgcgta
     1801 gaccgtccgc cggcgggggg aagcgcgtcc aggcccgccc ctgccaccca gcaacagcag
     1861 gccgcccaat acaagcgggg ctgtccaggc caccgtatcc atccaggggt cgcggtaggc
     1921 cgtgaggggg ccgaacacgg cgggcagggc cacaccgcac gccagcgcca gcaggtaccg
     1981 cccgaaccac agcagcatgc aggcgtacag cagcaccgca gccacgccca gcagcgccag
     2041 gtctacccag gcccgtagcc gagcgtcggg cgcgtaaata gagacttcct tgacaagcgt
     2101 gcccagaacg cggttgtcgc caggcacgac aagttgtggc gcggacagcg tgacggttgg
     2161 cgctcggctg gcagccggca cccacgcctg caagctccag ggttggccgg gctgcgtctc
     2221 gaccagggcc aggtcgctcc ctcccgcgga gagggtgagg gtgcgagggc cggggccgcc
     2281 ttccacaccc tccagcgtga cgtgcaccct acccggcccc cagttcggca gcacgaaccg
     2341 cgcgtcaggc tcggtccagc gatagatgcc ttgctcagcg ctcttctcag ggtcgtggaa
     2401 cccggcggcg gtgtagatgc ggtcggcctc cgtgccgatg cggaccgtct catctgccga
     2461 cactcggaag gccaggtacg ccatgcccag cacgaccgcg cccactgcta cgagcgcgac
     2521 aaggtgccgc cccaggctgg cagaggcgat ggcagcacga acgccggaag tgagggacaa
     2581 acgcaacggg gctgacagac ccaggccact cctgtgtgag gaaatacccc gccaagcggc
     2641 tgcgggacaa gtcattttat ggtaccctgt gggtgctgtc aatttggcac cgaagccaac
     2701 atatgagaag gagccttacc gatatgggca agatcataac ggagctagcc gttgccgaca
     2761 tggagcggtc gctgcaattc tacacggcgc tggggttcgt caaggacaac gaaggtatcg
     2821 ttgacgacaa tggagcgcaa tggtacagcc tcgcgctggg cgaaggctcc ctgtggctca
     2881 tacgtgccga cgtcaccgat gggctgatcg aggccgaccc gcgcggcaac ggcgtgacca
     2941 tctacatagc cgtggacgac atagacaccc tccacgagcg ggtcagcgac gcgggcctgc
     3001 tgatgaacat cgtcaagccg atagaaacaa tgtggtacgg cctgcgccag ttctcccttg
     3061 ccgaccccga cggctacatc ctcactctca accagccgat agagcaggca gatagcggtg
     3121 agggggaatc cggcgaagga caggcaagcg gtgagtagtc ccacaggtga aaagcagcca
     3181 acccctggag acaagccccc ccggcccagc gcggtccaag tcgcccccta cctgcaagcc
     3241 atcctcgacc acctctcggc cgccgacccc gtaatggccc gcctcgtcga ggagcgcggc
     3301 ccactaagca tcactgcctc gccccaggac gactactttt tcacggtgct cgacgccata
     3361 gcttcccagc agctttccag caaagtcgcc gccactatcg tcagccggat acgcgccctc
     3421 gtccccggcg aagatacgcc caccgccgcc cagattttcg ccctccctga tcaggctttg
     3481 cgcgacgtgg gcctctcctg ggccaaagtg agctacatca aggacctggc gcggcgggtg
     3541 gtctcgggtg agttggacct cgtgcacgtc tccgctatga gcgacgagga gatcatcaag
     3601 gagcttgtgg cggtgaaggg tatcggcagg tggaccgccg agatgttcct cctcttctcc
     3661 ctcgcccgct ccgacatatt cgcggtggac gactacggcc tgcgcgttgc cgtgaagcgc
     3721 ctctacgacc tgcccgacct ccccaagccc ggccccatgc gcgaaatagc cgaaccctgg
     3781 cggccctacc gctcctacgc ctccctctac ctctggcgca gcctggacaa ctcgcccaaa
     3841 gtcgaggcta tggaggtaaa agggagttag aatagacgaa aacgcaaagc atgccctgag
     3901 cgtagtcgaa gggacgcaaa ggacgcaaag aacgcgcaaa ggttaggatt gcttggggtg
     3961 tagggcacga tgcatcgtgc cctggtgagt agggtcccgg ctgaaccggg aggaccaaag
     4021 cggcctttgt catcctgagc gtagcgaagg atcagccgcc cgcgcttgat actcccttct
     4081 gaggcaacaa tgtgacccca gccaccctca tcctgtacgt ggaggactct cacctaccca
     4141 cctgatcctt cgctgcgctc aggatgacaa ggccacttgg gtcctgttgc accagacata
     4201 ggagctaccc acccaagcac agggcaccac gtgcctgcgt cagtactgga cttccaacga
     4261 gcctgcgccc ggtagggcca cgtccaggta caggctgcct tccaccattg aagtggtgaa
     4321 ggccaccgga gcgccaccca caagcacctg gcgtggggcc ttgtcgcccg gcaggagcat
     4381 gtgcagatgc accgatttag tctgcctgcc gccccaggcg agcgtaatgc tgccgtcggg
     4441 gtgtagctgc cagttgtagg cgaagtacgc gccgcttgcc ccgtagggga gggccacccg
     4501 cgccgacttc tgccccgtaa cggcccagcg gggggagagg gccactgcct cgtacagttt
     4561 gccgtcgtcc accacgcccg ccaggccctc ggtcagcgcg ttgagcatgg cagccgaacc
     4621 ccagccgtcg gtggcgagcg tttgaggatt acttacgccg gggtggcctt cggggtggta
     4681 ccacaagtag gagccgcccg tgcgctcggt caggtcccag tagcggcgca ggatgtcggc
     4741 cccgtaagcc tcaaagccgt gctcgaacgc cccgcgcgca agctccccac ccacgagggg
     4801 cataatgccc ccgttgaggt agtcccccgg cgggccgaac gtcctgggga acggcgggtc
     4861 gatgctgtac cactccgcga aggagcggcc cgcgttagct atgcgccttg cctggtacgt
     4921 ccgtagcact tccaccgcct gctcgtggct gagcgtaccc ctggtgagag cgtaggcgtt
     4981 ggacaggctc agttgcgtgc tctcgtccac gccggaaggg tccacgggcg tcagatgcac
     5041 ctggtgagtg taaaaatggc cgttccacgc cacacgattg aggtccagcc gcaacgcctc
     5101 ggcccgccca tcccacttag ccgcctctgt gtttctcccg aagtaccgct cgatgcccgc
     5161 cagcaacctc gccgcgcggt aggtcccggt gttgtcgcca tgcatgaggc tccagcgggt
     5221 gtgctcgtca atcgagcggc gcacggtgcc gaagtcgctg ccctgctcgt agtcccaggt
     5281 gtcgattgta aaggcgcgct ttaccagccc gtgctcggcg gaccagcggc gcggatcgtt
     5341 gaaagtatgt tccaggccgc gctccatcga gggtatcatg tcgcgcagcc aggcatcgtc
     5401 gcccgtggcc tgccaggcaa gccacacccc ctccacgaac aggtactccc ggtcggcctc
     5461 gatctctatc tggccgctgt agacgggcgt ggcgggcgtg tgcaggtaat agtcatcgaa
     5521 cgagccgtcc gcacgttgcg cgcgccgaaa gtgctccaac gcgctcgtca tgtccggctc
     5581 gaagtagcga gcgcccttca actggtgaac gtggtcgcgc agccagatgg acgcatcgtc
     5641 gggggagcgg tacccgcgca ccggggtcgt gttgtccggc gaggtgtatt ccaccacgtc
     5701 ctgcgccagg tagccctgca cgcgctggta gaggccgtcc cacacaacat cgcccgtgac
     5761 gatgcccaac tccggcggcg cgaccctcac cgcaccccac agcgcggcca cgatgcgccc
     5821 ctccctgtag agcgcgaaac tctgccgccc aggcgcgcct gctagcgtga cgctgagggc
     5881 ctttcccgcc tctaggggat atgtggcata caggcggttg tcgcctccaa gcacccgcac
     5941 ctcccccgct acctcactct cgacttccac agccgacagc agcggcaact cgctccccgt
     6001 gccccccaag cgcagattgg gagacggtac gtcgggccag gggtcgcgca acgtggggtc
     6061 gagggaggcc gcccgctcca ggccaagcag cgtgagcagc acctcgtacg gcgtgcccgc
     6121 gtgctccggg tgccattcga accgcgcccg ctcgaagtac tgcaccgtca acccgctcgc
     6181 ctcgtccttt agttcgggcg acaacggata gccgaattgc gcgagccttc catgtccctg
     6241 ccagtaagcc aggaacgccc tacccacgcg gtagcccgtt tcagggaaag taagctcgcc
     6301 cggaccggga ggtgcgctcg tcggtgagac tttgcgccct tcagccttca accgctccgc
     6361 acccagttgc cctagcaaaa cctcataagc cgaacctgcg ttttccgggt gcagctcgaa
     6421 gcggctgcgc tcgaagtact gcaccaggta gccgccttcg tagaacgcct ccgtcagcgg
     6481 gtagccaaac accggcacgc cgccatgagc ctgccagtaa gcgaggaact tgccctcgat
     6541 gttgtgcccc gtcggcgcga agtaaagacg gttggcgggt agtaagggct gccgtgccct
     6601 cgcgcctgcg accaacgagg gaccaggagc gcgcggaaat agcaacgtga gtataaggaa
     6661 gacccccgct gccatagcag gaagcaaacg gccacgcctt ataaccgagg tccatcccac
     6721 tacagatagc cttccgacac aagcacctct atcaacagag gctcacctgt atacagcata
     6781 atcacgctat ccccaacctc aatgacaggc agcaggtcat accccttctc caggaactcc
     6841 tcgcgggcct cggcatcccg gctgatgttg cgttccacgt gaggcacgcc cattcgcgaa
     6901 agaaactcct tcgcagcccg gcacggccct cagaggttgc ccgaatatag cacgacaggc
     6961 ggcttgtcca tccaccctcc tcggcacaag cgaatatgct gctagaatag cacaggcaag
     7021 tgccgagtgc cgagtgccga gtgccgagtg gtcagtatta ccctggatac atccagaaca
     7081 attctaacct ttgcgcgtcc
//
LOCUS       DASRWZ010000600         3733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_392467, whole genome shotgun sequence.
ACCESSION   DASRWZ010000600 DASRWZ010000000
VERSION     DASRWZ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392467"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1969)
                     /locus_tag="VFH60_09465"
     CDS             complement(110..1969)
                     /locus_tag="VFH60_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6314052.1"
                     /translation="MSGLSPNNGIAPSVASPPKPLKTWQYIGRLWRFRPWLLLANALI
                     GAVFAIAPIVMGLVVQAIFNSLTGDAPVSLGIWELVALLVLAAFANYALSYFRIFADI
                     AIEFSTGSLLRKNMFEHILRLPGAQAIPDSPGEAISRFRDDSGEIMGTIWWPMILLGE
                     VLFAVGAFVVLAGISLPITLIVFLPLLVVVAAANMASARILKYRRASRLTTGNVTGFL
                     GEMLGAVQAIKVADAEESMLAHFDRLNDMRRTETVRDRLFNQVMSSVYGNAVSLGTGL
                     ILLFAARSMSAGEFTVGDFALFQFYLGGLVWLPFIVGELIARYRQATVSFERMNRLMQ
                     GAQPEALVQPGPVYLNEDPPEIQHLPRTDEHRLETLEAAGLTYRHPGSGRGIEGVSLR
                     LQRGSFTVITGRVGAGKTTLLRVLQGLLPRDAGEIYWNGQRVDDPASFFVPPRSAYTP
                     QVPHLFSERLRDNILMGLPPDSVDMEEAIRLGVFSEDVGALEAGLDTVVGPRGVKLSG
                     GQAQRTAAARMFVRDPELLIFDDLSSALDVQTERTLWERLFERQQATCLVVSHRRTAL
                     RRADQVIVLKDGRVVAQGKLDDLLATSEEMRRLWRGEHDAPAREEVPVHSPDA"
     gene            complement(1966..>3733)
                     /locus_tag="VFH60_09470"
     CDS             complement(1966..>3733)
                     /locus_tag="VFH60_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6314053.1"
                     /translation="GKNRRRMRLRATVRQYWSLFSGYLRPQRGRVLVLGLLLFGSTGL
                     QLLNPQVVRYFIDTATRAGAADLLVGAAALFLGIAVLQEVAAVLATYVGENVAWTATN
                     SLRRDLAAHCLRLDMSYHNAHTPGELIERIDGDVSTLANFFSLFALRVVGNGLLVVGI
                     LVFLLLEDWRISLALGLLAMVGIVVLNRLRELATPHWVRGRQASANLAGFLEERLAGT
                     EDIRANGATPYKMNMLFRLMRDLMQRYRRARVIGHLGSVLGHFISEMGLAIGLGLGAY
                     LYLEGSMSIGTVYMVSFYAGMLALPLRRITDELEDLHKAGATILRVEELRQTPIAVMD
                     GRGVPLPQAALGVEFDRVSFGYAVGVPVLRDLSFSLGPGKVLGLLGRTGSGKTTVTRL
                     LLRIYDPDEGAVRLGGADLRDLKLGQVRSRVGMVTQEVQLFHASVRDNLTFMNANVPD
                     ERLLAAIKQLGLTEWYERLPEGLDTKLAPSGGLSAGEAQLLAFTRVFLKDPSLVILDE
                     ASSRLDPATERLIERAVGRLLEGRTAIIVAHRLGTVQRADEIMILEQGCILEHGRREQ
                     LALDTGSRFSELLRTGMEEALA"
BASE COUNT          684 a         1299 c         1154 g          596 t
ORIGIN      
        1 ctactggcga gggcaaaagt cagccagcat cctgtggctc aacaacgata tttatcgaac
       61 aactttccgc gattataaac gaaacgcacc aagcgtgacc acgctcactt cacgcgtcgg
      121 gagaatggac cggaacttcc tccctcgcgg gtgcgtcgtg ctctcccctc cacaggcgtc
      181 gcatctcctc ggaggtggcg agcaggtcgt ccagcttgcc ctgggcgacc acccggccgt
      241 ccttcagcac gatcacctgg tcggcgcggc ggagggcggt gcggcggtgg gacaccacaa
      301 ggcacgtcgc ctgctgccgc tcgaacagcc gctcccacag cgtgcgctcc gtctgcacgt
      361 caagcgcgct ggagagatcg tcgaaaatca gcagttcggg gtcgcgcacg aacatcctgg
      421 ccgccgccgt cctctgcgcc tgcccgcccg acagcttgac ccccctcggc cccaccaccg
      481 tatcgaggcc cgcctccaac gcgcccacgt cctccgagaa cacgcccagc cggatagctt
      541 cctccatgtc aacgctgtcc gggggaaggc ccatcaggat gttgtcgcgc agccgctcgc
      601 tgaacaggtg cggcacctgg ggcgtgtagg cgctacgggg cggcacgaag aaggaggcgg
      661 ggtcgtccac gcgctgcccg ttccagtata tctcgcccgc gtccctcggc aacaagccct
      721 gcaacacgcg caggagggtc gtcttgcccg cgcccaccct tcccgtaatc accgtgaagg
      781 agccgcgttg cagacgcaaa ctcaccccct ctatgcctct gccggagccg gggtggcggt
      841 acgtgaggcc ggcggcctcc agcgtttcga ggcggtgctc gtcggtgcgg gggagatgtt
      901 gtatttccgg cgggtcctcg ttgaggtaga cgggtccagg ttgtaccagg gcttcgggct
      961 gtgcgccctg catgagcctg ttcatgcgct cgaacgacac cgtcgcctgc ctgtacctgg
     1021 ctatcagctc gcccacgatg aagggcagcc acaccagccc gcccaggtag aattggaaaa
     1081 gcgcgaaatc gcccaccgta aactcgcccg ccgacatcga ccgcgccgcg aacagcaata
     1141 ttagccccgt gcccaggctc accgcgttgc cgtagaccga ggacatcacc tggttgaata
     1201 ggcggtcccg caccgtctcg gtgcggcgca tgtcgttgag cctgtcgaag tgggccagca
     1261 tgctttcctc cgcgtcggcc acctttatcg cctgcaccgc gcccagcatc tcgcccagga
     1321 agccggtgac gttgcccgtg gtaaggcggc tggcgcggcg gtacttgagg atgcgcgcgc
     1381 tcgccatgtt ggcggccgcc accaccacga gcagcggcag gaacacgatc agggtgatgg
     1441 gcaggctgat acccgccagc accacgaacg cccccaccgc gaacagcacc tcccccagca
     1501 gtatcatcgg ccaccagatt gtgcccatta tctccccgga gtcgtcgcgg aaccggctga
     1561 tagcctcgcc gggcgagtcg gggatggcct gcgcgcccgg cagccgcaag atgtgctcga
     1621 acatgttctt gcggagcagc gagccggtgg agaactctat cgctatgtcc gcgaagatgc
     1681 ggaagtagga gagggcgtaa tttgcgaaag ccgccaacac cagtagcgcg accaactccc
     1741 atatgcccag gcttaccggc gcatccccgg tgagcgagtt gaagatagcc tgcacgacga
     1801 ggcccattac gatgggcgcg atggcaaaca cagcccctat caaggcgttg gccagcaaca
     1861 gccacggccg aaaccgccac agccgcccga tgtactgcca ggtcttcagc ggctttgggg
     1921 gagacgcaac gctcggcgct ataccgttgt taggagatag accgctcatg ccagcgcctc
     1981 ctccatgccc gtgcgcaaca actccgagaa gcgggagcct gtgtcgagcg caagctgctc
     2041 tcgccgcccg tgctccagta tgcacccctg ctccaatatc atgatctcgt cggcccgctg
     2101 cacggtgccc agcctgtgcg ccacgataat agctgtacgc ccctcgagca gccttcctac
     2161 cgcccgctct atcagccgct cggtggccgg gtcgaggcgc gacgaggctt cgtccaggat
     2221 caccaggctg gggtctttta gaaagacgcg cgtgaaagcc agcagttgcg cctcccccgc
     2281 cgacagcccg ccggagggtg ccagtttggt atcgagaccc tccggcagcc gctcgtacca
     2341 ctccgtgagg ccaagctgct taatggcggc aagcagtcgc tcgtccggca cgttggcgtt
     2401 catgaaggtg aggttgtccc gcacgctggc gtggaacaac tgcacctcct gcgtcaccat
     2461 gcccactcgc gagcgcacct ggcccaactt caggtctcgc aggtcggcac cccccagcct
     2521 gaccgcgccc tcgtcggggt cgtagatgcg caggaggagg cgcgtcaccg tagtcttgcc
     2581 ggagccggtg cgccccagca ggcccagcac cttgcccggc cccaggctga acgacaggtc
     2641 gcgcaacacc ggcacgccca ccgcgtagcc gaacgataca cggtcgaact ccacgcccaa
     2701 ggcggcctgg ggaagcggca cgccgcggcc atccatgacg gcgatggggg tctgacgcaa
     2761 ctcttcgacg cggaggatgg tggccccggc tttgtgcagg tcctctaact cgtcggtgat
     2821 cctgcggagg ggcagggcca gcatacccgc gtagaagctg accatgtaga cggtgcctat
     2881 gctcatcgaa ccttccaggt agaggtaagc gcccagcccc aggccgattg ccagccccat
     2941 ctcggagatg aagtggccca gcaccgagcc gaggtggcct attacgcgcg cgcggcggta
     3001 gcgctgcatc aggtcgcgca tgagccggaa gagcatgttc atcttgtagg gggtggcccc
     3061 gttagccctg atgtcctcgg tgcccgccag gcgctcctcc aggaagcccg ccaggttcgc
     3121 gctcgcctgc ctgcccctca cccagtgggg agtggcaagc tcgcgcaggc ggttcagcac
     3181 tactatgccc accatcgcca gcagccccag cgcgagtgag atgcgccagt cttccagcag
     3241 cagaaacacc agtatgccca cgaccagcag gccgttgccc accacgcgca aggcaaagag
     3301 cgagaagaag ttggccagcg ttgacacgtc gccgtctatg cgctctatca actcgcctgg
     3361 cgtgtgggcg ttgtggtaag acatgtcgag gcgcaggcag tgggcggcca ggtcgcggcg
     3421 cagcgagttg gtggccgtcc aggcgacgtt ctcgcctacg taggtagcca gcaccgccgc
     3481 cacctcctgt agcaccgcga tacccaggaa gagcgccgct gcccccacca gcaagtcggc
     3541 agccccggcg cgggtggcgg tgtcgatgaa atagcgcacc acctgcgggt tcagcagttg
     3601 cagccccgtc gagccgaaca acaacagccc cagcaccaac acgcggcccc gctggggcct
     3661 caagtagccg gagaagagcg accagtattg ccggacggta gcccttaagc gcatacgcct
     3721 cctgttcttg ccg
//
LOCUS       DASRWZ010000601         1706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_254011, whole genome shotgun sequence.
ACCESSION   DASRWZ010000601 DASRWZ010000000
VERSION     DASRWZ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254011"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..>1706
                     /locus_tag="VFH60_09475"
     CDS             285..>1706
                     /locus_tag="VFH60_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HET6314054.1"
                     /translation="MAYQPQNPLIVQSDRSLLLEVDNPLYSATRDELARFAELEKSPE
                     HIHTYRITPLSLWNAASAGISIEAILGTLERYSKFDLPGNVITDIKDYVSRFGRIKLL
                     KEGDHLLLRSEDPMLIAEVARNKKIQPCVLAQPDRNTLQIDPRMRGHVKHALVLFGYP
                     AEDLAGYTDGAALDIVLREDSARDAAAFGLRPYQQNAVSAFWAGGTVHGGSGVVVLPC
                     GAGKTVVGMGTMSQAQTHTLILTPSTTAVRQWIAELLDKTNLTPDQVGEYTGEKKEIK
                     PVTVTTYQILTYRPSVSMVNEQTGEIGEFPHFDLFTRHDWGLIVYDEVHLLPAPVFRV
                     TAEIQARRRLGLTATLVREDGRETDVFSLIGPKKFDVPWKDLEAQGWIATAECTEVRM
                     PLAPEERMAYTLVDDNRIKYRIAAENPLKMDALEVLLERHQADSVLVIGQYIEQLKEI
                     SKRFEAPIVTGKTPNVERDRLYEQ"
BASE COUNT          364 a          566 c          498 g          278 t
ORIGIN      
        1 agtaccggga cagtaaggaa ggcgtatata gggccttaaa catacgtgga agttcaattg
       61 gagagcaata aatggtaact gtgacccagc ttcgtcagaa gatgttttgc acatccggcg
      121 ctccgtttcc acatccgcca ccctttggta aggccggtac ccttgtggta caattagaat
      181 ccgcggatag tctactgtgc gcggaaagaa cgctgcgttg accctattta ttgtcagcaa
      241 agtagctgat atccggcgcg cttggcggaa agctgaggaa tagcatggct taccaacccc
      301 agaaccccct catcgtgcag agcgaccgct cgctcctgct cgaagtcgat aacccgctct
      361 actcggccac ccgcgacgag cttgcacgct tcgcggaact cgaaaaaagc ccggagcaca
      421 tccacaccta tcgcatcacg cctctctccc tctggaacgc tgcctccgcg ggcatcagca
      481 tcgaagccat acttggcacg cttgagcggt acagcaagtt cgacctcccc ggcaacgtca
      541 tcaccgacat caaggactac gtttcccgct tcggtcgcat caagctgctc aaagagggag
      601 accacctgct gctgcgctcc gaagacccga tgctcatagc cgaggtcgcc cgcaacaaga
      661 agatacagcc gtgcgtgctc gctcagcccg accgcaatac gctccagatc gacccccgca
      721 tgaggggcca cgtcaaacac gccctggtac tgttcggcta cccggctgaa gacctcgcgg
      781 gctacaccga cggcgcggcc ctggacatcg tgctgcgcga ggacagcgcc cgcgacgccg
      841 cggctttcgg cctgcgcccc taccagcaga acgcggtgag cgccttctgg gcgggaggca
      901 cggtgcacgg gggtagcggc gtggtggtgc tcccttgcgg ggcgggcaag acggtggtgg
      961 gcatgggaac catgtcccag gcgcagaccc acaccctcat cctcaccccc tccaccaccg
     1021 cagttcgcca gtggatagcc gaattgctcg acaagacgaa cctcaccccc gaccaggtgg
     1081 gcgagtacac cggcgagaag aaggaaatca agccggtcac cgtcaccacg taccagatcc
     1141 tcacctaccg gccctccgtg tcgatggtga acgagcagac cggcgagatt ggcgagttcc
     1201 cgcacttcga cctgttcacc cggcacgact ggggcctcat cgtctacgac gaggtgcatt
     1261 tgctgcccgc cccggtgttc cgcgtgaccg ccgagataca ggccagacgc cgcctgggcc
     1321 tcaccgccac gctggtgcgc gaggacggac gcgagacgga cgtgttctcc ctgatcggcc
     1381 ccaagaagtt cgacgtgccc tggaaggacc tggaagccca gggctggata gccaccgccg
     1441 agtgcaccga ggtccgcatg cccctcgccc ctgaggagcg catggcctac acgctggtgg
     1501 acgacaaccg catcaagtac cgcatcgccg ccgagaaccc cctcaagatg gacgctctgg
     1561 aagtgctgtt ggagcggcac caggccgact cggtgctggt gatagggcag tacatcgagc
     1621 agctaaagga gatcagcaag cgattcgagg cgcccatcgt cacgggcaag acgcccaacg
     1681 tggagcgcga caggctgtac gagcag
//
LOCUS       DASRWZ010000602         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_117521, whole genome shotgun sequence.
ACCESSION   DASRWZ010000602 DASRWZ010000000
VERSION     DASRWZ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117521"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1671)
                     /locus_tag="VFH60_09480"
     CDS             complement(94..1671)
                     /locus_tag="VFH60_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6314055.1"
                     /translation="MSEVASSPKYEAPPNGFRTFVAIWVTQAFSAFGSQLTFFALTIW
                     LAGDLYSRDEQKPQLAFALSAISLAFTLSVLIAPIAGAWADRHDRKRTMLLVDVANGV
                     LSLVLMGLIMSGMLELWMLLVLFPIVAVLFQFHNAAFDASYAMLVPEKQLPRANGMMQ
                     TMWSLSAVLSPAIAAGIISIPELLRGTLPPGYDPREGIRSGAALAIGVDAVTFFIGAA
                     VLPFLHVPSPKRTDLGETGAKPTKSIWVDIQEGARYIRVRKPLLWLLATFTIINFASA
                     PLEVFIPLLLKFNLAADWEAQGFTFASAMAFLSTFGGLGGVAGGILMSTWGGLKKRRV
                     YGVLVPIIIAGTIQAFYGLSPWLYVTAGLNFLLIALMPIMNAHSQTIWQTQVPRELQG
                     RVFSVRRVIAQFSAPIGVLVAGVTGAYFNPGWVLAILGIAVVIFGIFQLFNPYLLRVE
                     DKAFLDKLAGDGGEGGPAGGNGTVTGADLNSAMEQPSDGDVSLGSPTSVPVMPTPNDD
                     IAPSEDETDRLAGYSKR"
     gene            complement(1839..2270)
                     /locus_tag="VFH60_09485"
     CDS             complement(1839..2270)
                     /locus_tag="VFH60_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314056.1"
                     /translation="MILIDLAAYAHDTNAQMEEVLTLNSFYHPREHDQEGHTHSGPHD
                     GSDHARGHEGHEAYGYDASHSGHAGHAAPAEHADLRPDDTAQETGAEPPAPGSEYSDK
                     DFGLYQVLLAVERGELSPEDAARKLEELEAGGQEEGGTGPI"
     gene            complement(2479..3579)
                     /locus_tag="VFH60_09490"
     CDS             complement(2479..3579)
                     /locus_tag="VFH60_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET6314057.1"
                     /translation="MVEKDRDITTIRTEEFTETPRLVLEQPVGDISIQGWDNTAIEIS
                     TGDDDDDYDVEVTGSQVTLRNRRRKVHIGEGFEGPWRELGNLGVDVERVARRVEKQVE
                     RGMRRFKGINIDLDLGRWSHSHDFTIKVPHNCDLMLRTSTGDMYVKDVNGTLFIQTTS
                     GDLNLDSLSGNLLVSTASGDIHVRGMEGKVGARSASGDITLERGDLQELGVQTASGDI
                     NVYLKRVPEREFDVRTVSGDLRVTLPNDARLTAEMTTVSGDLRCKLPHEIISRSGRRT
                     RTLVVNGGGPAARFHSVSGDVSVMPDRFEGGARTSDLSRAEGESDGSEEDIRVPEGEV
                     SREQAQLDILQAVQRGEITSQEALRRLSSMGR"
BASE COUNT          652 a         1120 c         1174 g          703 t
ORIGIN      
        1 aacagcgctg ttccaacttc gcggctgaat tgacgtatga gatgagtacc gcgcaagctc
       61 atacgctcat gcctccacgt tccgtttacg agcttacctt ttgctgtagc ccgccagccg
      121 gtcggtctcg tcttccgacg gggctatgtc gtcgttgggg gtcggcatga caggtaccga
      181 ggttggcgag cctagcgaca cgtcgccgtc ggagggttgc tccatggccg aattaaggtc
      241 tgcgccggtg acggtgccgt tccctccagc cggtccgccc tctccgccgt cccccgccag
      301 cttatcgagg aacgccttat cctccacgcg caacaggtag gggttgaaca gttggaagat
      361 gccgaagatg acgacggcga tgcccaggat cgcaagcacc cagccagggt tgaagtatgc
      421 gccggttacg cccgccacga gcacgcctat aggggccgaa aactgggcga tgacgcgccg
      481 gacggagaag acccggccct gtaactccct gggcacctgc gtttgccaga tcgtctgcga
      541 gtgggcgttc atgatgggca tcagggcaat caacaagaag ttgagtcctg ccgtgacgta
      601 cagccagggc gacaggccgt agaacgcctg tatcgtgcct gcgattatga tgggcaccag
      661 cacgccgtaa actctgcgct tcttaaggcc gccccaggtg ctcatgagta taccgcccgc
      721 taccccaccc aggccgccga acgtgctcag gaaagccatg gccgaggcga aggtaaagcc
      781 ctgcgcctcc cagtcggcgg ccaggttgaa cttgagcagg agcgggatga acacctccag
      841 gggcgcgctg gcgaagttga tgatggtgaa ggtcgccagc agccagagca acggcttgcg
      901 cacccgtatg tagcgtgcgc cttcctgtat gtcaacccaa atgctcttcg tgggcttggc
      961 acctgtctcc ccgaggtcgg tgcgcttggg cgagggcacg tgcaggaacg gcagcaccgc
     1021 cgcgccgatg aaaaacgtta cggcatccac acctatcgcc agggccgcgc ccgaccgtat
     1081 gccttcccgg gggtcgtagc cagggggaag agtgccccgc aatagctcag ggatggaaat
     1141 gataccggcg gcgatggcgg gcgagaggac ggccgagagc gaccacatcg tctgcatcat
     1201 gccattggcg cggggtagct gcttttcggg caccagcatg gcgtaggaag cgtcaaaggc
     1261 cgcgttgtgg aactgaaaga ggaccgctac tatagggaag aggaccagta gcatccagag
     1321 ttccagcatc ccggacatga tcaagcccat cagtaccagg ctgaggacgc cattggctac
     1381 gtcaaccaag agcatcgtgc gcttgcggtc gtgccggtcg gcccaggccc ctgcgatggg
     1441 ggctataagg accgaaaggg taaaggccag gctgatggcg gagagggcaa aagccagttg
     1501 gggcttctgc tcgtcgcggc tatagaggtc tccggcgagc caaatggtca gggcgaagaa
     1561 ggttagctgg ctaccaaagg ccgagaaggc ttgagtgacc cagatggcga cgaaggtacg
     1621 gaagccgttg ggcggggcct cgtacttcgg gctgctagca acttcagaca tggtgcatct
     1681 ctctcctgcg gtgtggattg cggttctcgt aggtcccgtg cagtaggacg tattgcgagc
     1741 aagaggcaga acggacagcg ccggtccacc cctgaataat actcgaacgt agataaacgt
     1801 ctatcggtct agggaagtaa agcgcgaggt acgcggccct aaatcggccc cgtaccgccc
     1861 tcctcctggc cgccggcttc caactcctcc agcttgcggg cggcgtcctc cggcgatagc
     1921 tcgccacgct ccacggccag tagcacctgg tacaatccga agtctttgtc cgagtactcc
     1981 gagccgggcg cgggcggctc tgcgcctgtc tcctgtgccg tgtcgtcggg ccggaggtca
     2041 gcgtgttcgg ccggggctgc atggcctgca tggccggagt gagaggcatc atagccgtat
     2101 gcctcgtgtc cttcgtggcc gcgagcgtgg tccgagccgt cgtgcgggcc ggagtgcgtg
     2161 tggccctcct ggtcgtgttc gcgcgggtgg taaaagctat tcaaggtgag cacctcctcc
     2221 atctgtgcgt ttgtatcgtg ggcgtaagcc gcaagatcta taaggatcaa ctctgtcctc
     2281 ctggtccgcc agcgcacact agtcatgcgc actacggggc acgttttggg acgcaccgaa
     2341 cccgcctggc atgtatagca cgccaggcgc tcgccttgag ccgggtgccg gtcgcggcgc
     2401 tacgttcggc tacgttgcga tagggtgaga gtggaggcga tgcgcaacag ctgagcactc
     2461 gcctccacgc gattgtgtct agcggcccat gctgctcagg cggcgcaggg cttcttgcga
     2521 agtgatctcg ccgcgctgca cggcctggag aatgtcgagc tgggcctgct cacgagagac
     2581 ctcgccctca gggacccgga tgtcctcctc ggaaccgtca gactcgccct cggcgcggga
     2641 caggtcggac gtgcgcgcgc ccccctcgaa gcggtcgggc ataacgctca cgtcgccgct
     2701 gaccgagtgg aagcgagcag cggggccgcc gccgttcacc acgagggtgc gggtgcgccg
     2761 ccccgagcgg ctgatgatct cgtggggcag cttgcagcgc aggtcgccgc ttaccgtggt
     2821 catctcggcg gtgaggcgtg cgtcgttggg cagggtcacg cgcaggtcgc cgctcaccgt
     2881 gcgtacgtcg aactcgcgct cggggactcg cttcaggtag acgttgatgt cgccgctggc
     2941 ggtctgcacg cccaactctt gcaggtcgcc ccgctccaac gtgatgtcgc cgctggcgct
     3001 acgcgcgccc accttgccct ccatgccccg cacgtggatg tcgccgctgg cggtgctgac
     3061 gagcaggttg cccgaaaggc tgtccaggtt gaggtcgccg gaggtggtct ggataaagag
     3121 ggtgccgttc acgtccttga cgtacatgtc gccggtgctg gtgcgcagca tcagatcgca
     3181 gttgtggggc accttgatcg taaagtcgtg gctgtgcgac cagcggccga ggtcgaggtc
     3241 tatgttgatg cctttgaacc ggcgcatgcc tcgctcgacc tgcttctcca cacggcgggc
     3301 cacccgttcc acatcgacgc ccaaattacc aagctctcgc caggggcctt cgaagccctc
     3361 gccgatatgt actttgcgcc gcctgttacg cagcgtgacc tgggagcctg tcacctcgac
     3421 gtcgtagtca tcgtcatcgt cgccggtgct aatctctatt gcggtgttgt cccacccctg
     3481 gatggagatg tctccaacgg gctgctcaag cacgagtcgc ggcgtctcgg taaactcttc
     3541 tgtcctaatc gtggttatat cgcggtcttt ctctaccatc gtgtgttcct ccattcgtct
     3601 gctactagac tgagtcttct atacggattt ctcaatccaa tatccaaaa
//
LOCUS       DASRWZ010000603         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_597622, whole genome shotgun sequence.
ACCESSION   DASRWZ010000603 DASRWZ010000000
VERSION     DASRWZ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597622"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFH60_09495"
     CDS             <1..1215
                     /locus_tag="VFH60_09495"
                     /inference="COORDINATES: protein motif:HMM:NF027495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HET6314058.1"
                     /translation="MPTLQDYNQFEGAHWEMGAVRNVLAHQGVVAPHTGEPFTEAMLL
                     GISGGVSVLYFAFDYEGYEPNVFIGTHLASPTDRPMEAVLERLGVKGHARETTSRDRA
                     RANVLEALEEGTPVIAWAGQRGLLYNAMPPGLDPGMMPLVVYGYEPGEGTVRIADRAR
                     VPLTASASEFDDARAAQGSLKHRSLTLEAPPRLKNLSGAIEAGVRACARVMLEGPEKG
                     PRANFGLAALRKWAGLVVDTKSPRGWLGTFAPGPHLYHALASCFEWIEIRGSGAGAGR
                     TMYADFLTEAATALHKPQLEEASEKYRESARRWTGLARTMLPDEMPLLKETRELLESN
                     HRLFVEQGMASLPERLGMARRLEEIKGQAAHSFPRAENELVPFLEDIRDHILAVHDAE
                     KAAVEAMQQAVG"
     gene            1314..2192
                     /locus_tag="VFH60_09500"
     CDS             1314..2192
                     /locus_tag="VFH60_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314059.1"
                     /translation="MFRFSLFAAVAVVVVALIMLVLARPTTPSLSEAEVSGTMEAVQV
                     ATSQAEGRATARAGRASGTARARATTMAEARERGAIATQAALATAEAPPTLTAEARAY
                     ETARVQAEATAQVLEEQAILVYGLTTGTLEQMEGDEVPCAAAGVNLRDFVAEVTFRNP
                     QAAGAEADRAWDYGLVFSNIGEGTEYRAILDSGGSWTFGLHSEDYDISISDATGLVDL
                     SGAGSNTLKLFVTGDEAQLYVNGSYIDTFDLTMLALGQSSEAAHDVMVCAGLGERYRG
                     VGMVTQYEEFKVWFLP"
BASE COUNT          484 a          792 c          820 g          420 t
ORIGIN      
        1 atgcctaccc tgcaggatta caaccagttc gagggggcgc actgggagat gggtgcggtg
       61 cggaacgtgc tggcgcacca gggggtggtc gcgccgcaca cgggggagcc gttcacggag
      121 gctatgctgc tgggtatcag tgggggcgtg agcgtcctct acttcgcgtt cgactacgaa
      181 ggctacgagc cgaacgtctt catcggcacc cacctggcct cgcctaccga caggccgatg
      241 gaggcggtgt tggagcgctt aggcgtgaaa gggcacgcac gcgagaccac cagccgagac
      301 cgggcgcggg ccaacgtgct ggaggcgctg gaagagggga ctcccgtgat tgcctgggcc
      361 gggcagcgtg gcctcctgta caatgccatg cctcccggcc tcgaccccgg catgatgccc
      421 ctggtggtct acggctacga gccgggggag ggcaccgtcc gcatagccga tcgcgcccgc
      481 gtgcccctca ccgcaagcgc aagcgagttc gacgacgccc gcgccgccca gggcagcctg
      541 aagcaccgca gccttacgct tgaagccccg ccccggctga agaacctctc cggggcaata
      601 gaggcggggg tgcgggcctg cgccagggta atgttggagg ggccggagaa aggacccagg
      661 gccaacttcg ggctggcggc gctccgcaaa tgggccgggc tggtggtgga caccaaaagc
      721 cccaggggct ggctgggcac cttcgccccc ggcccccacc tgtaccacgc cctcgccagt
      781 tgcttcgagt ggatagagat acgcggcagc ggggcaggcg cgggccggac catgtacgcc
      841 gacttcctca ccgaagcggc aaccgcgctc cacaagcccc aactggaaga agcctccgag
      901 aagtaccgcg agagcgcccg gagatggacc ggcctggccc gtaccatgct ccccgacgaa
      961 atgcccctgc tcaaagagac gcgggagctt cttgagagca atcaccgcct gttcgtggag
     1021 cagggcatgg cctcgctgcc ggagcggcta ggtatggccc ggcggttgga agagatcaag
     1081 ggccaggcgg cacatagctt ccccagggcc gagaacgagc ttgtgccctt cctggaggat
     1141 atacgcgacc acatcctggc agtgcatgac gcggagaaag cggcggtgga agccatgcag
     1201 caggccgtgg gctgacgcta cccgtgcacg aaacgcccat acagcgagat atgcctgaga
     1261 acgagccgga acgcccatcc caccgtgtcc aggcagagcg gcggagcagg cttatgttcc
     1321 gctttagcct cttcgctgcc gtggccgtcg tagtcgtcgc gctgataatg ctggtgctgg
     1381 cgcggcctac cacacccagc ctctcggagg cagaggtcag cggcacgatg gaggcggtgc
     1441 aggtcgccac gtcgcaggcc gaggggcggg ccacggccag ggcggggcgg gcaagcggca
     1501 ccgccagggc aagggcaacc acgatggcgg aggcccgcga gcggggagcc atcgccaccc
     1561 aggctgccct cgccaccgca gaagcgcccc ctaccctcac cgccgaagcc agggcatacg
     1621 agaccgcccg cgtgcaggcc gaagctacgg ctcaagtgct ggaagagcaa gctatcctcg
     1681 tttacggcct cactaccggc acgctggaac aaatggaagg ggacgaggtg ccctgcgctg
     1741 ccgccggtgt caacttgcgc gattttgtgg ccgaagtgac gttccgcaac ccgcaggccg
     1801 ccggcgcaga ggcagaccgg gcgtgggact acggcctcgt cttctccaat atcggagagg
     1861 gcaccgagta ccgtgctatc ctcgactccg gcggctcctg gacgttcggc ctgcacagcg
     1921 aggactacga catctctatc tcggatgcaa ccggcctggt ggacctctcc ggcgcaggct
     1981 ccaacacgct caagctcttc gtgacgggcg acgaggccca actctacgtc aacggaagct
     2041 acatcgacac cttcgacctc accatgttgg ccctggggca gtcgagcgag gcagcacacg
     2101 atgtaatggt gtgtgcgggg ctgggggaga ggtacagggg tgttgggatg gttacgcagt
     2161 atgaggaatt caaggtgtgg tttttgccat aactggtaat caagcgtggt tacgtagtgt
     2221 tttccaggta atctgcctct atccgctcgt tctcagcatc ttcttcagct atgtcgttct
     2281 ctatcaactg cgagttagca tgataatatg ccagtgccgc atacacctgg gctatattca
     2341 agtggtctat ttcctcaact gatacacgga cgaccaccag acacatccgg caagatggtt
     2401 gttagcgtcc caatatcgat agatggtgta actgccattt tccgctccgc cgcttattca
     2461 ttccaggtgc tcagatactc aactctgttc ttcatttcct ccgcagacaa tgcaag
//
LOCUS       DASRWZ010000604         5982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1008041, whole genome shotgun sequence.
ACCESSION   DASRWZ010000604 DASRWZ010000000
VERSION     DASRWZ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008041"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /gene="raiA"
                     /locus_tag="VFH60_09505"
     CDS             complement(<1..623)
                     /gene="raiA"
                     /locus_tag="VFH60_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET6314060.1"
                     /translation="MSVNIRPKNFKLEPKIEEHIRKHLTKRNRQVDDLESTEVVLSQQ
                     PTRHNAQRFQYVAQVTLHTRHNIVRSEVAHDELLTAVDQALANLDKQLERRKTRQSRN
                     KKGGQGLGRSAAMNVSTEEPSVIEMAAPAKPAAEAFAGNGKYGGSDEGAIVRVKRFRM
                     KPMDPEEAIEHMELLGHNFYVFCNSDQERVGVVYRRNDGDYGLLEPEL"
     gene            complement(743..815)
                     /locus_tag="VFH60_09510"
     tRNA            complement(743..815)
                     /locus_tag="VFH60_09510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(780..782),aa:Arg,seq:cct)
     gene            complement(836..1750)
                     /locus_tag="VFH60_09515"
     CDS             complement(836..1750)
                     /locus_tag="VFH60_09515"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HET6314061.1"
                     /translation="MSSTNGRRQKGLLASAPSMADAPWELPGYLMIIGGADRLDHTGI
                     LAKLFLQLVERAERNSEQRDIVLITTATRHPEILKNEYVRIYTRHGYDPARIHAPLIR
                     NHDEAMDEHTVGLLDRAAGIFITGGDQYALTQVLDRTHAEKAIMAAYGCGAVVAGTSA
                     GATAMGRPMVVAGGGTGELRMGMVQMSNGLGWAGDDLIIDTHFGARGRFPRLTASVAE
                     HPSALGVGIDENTCLLLDCHGRGIVKGTGVVYFVDASHAQVNTAHGTFPGTPISVGPL
                     EVAVLSSGGEYDMRARRVLLEMGAPSRV"
     gene            complement(1836..4685)
                     /gene="cphA"
                     /locus_tag="VFH60_09520"
     CDS             complement(1836..4685)
                     /gene="cphA"
                     /locus_tag="VFH60_09520"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET6314062.1"
                     /translation="MSEIKVKKIKVLSGANIWAYRPVLEILADIGKYEELPSDKLPGF
                     TERLVEAIPSLWEHRCSEGQPGGFLERLRMGTYMGHIIEHIILELQSLAGLDVGSGRT
                     RGTAHYGEYRIVVDYKDPDAAKQAAYLAIEMAEKLAEGQPLDFDLAERIEQIRDTAED
                     NMLGPSTQTIVDAARKRRIPWMRLNRAALVQLGHGKYAKRIQAAETSFTANIGVEIAS
                     DKELTKDLLAKVGVPVPEGEVVDNADDAWRAAQDLDGPVVLKPFDGNQGKAVSVNLTT
                     EEQVRKAFELAQQHSDAVIVERFLSGTDHRLLVVNGKLVAASQRRPPQVVGDGRSNVR
                     ELVKRVNEDPRRGAGHGSALTRIKMDAAAELTLGKQGHTWETVPDAGQVVFLRDNSNL
                     STGGTAIDVTDIVHPDNEAIAVLAASTIGLDIAGVDVVCESIKRPLQDQNGGIVEVNA
                     APGLRMHVYPSEGRSRPVGEAIVDMLFPEGTPARVPLIAITGTNGKTTVTRMIAHIYS
                     TMRKFVGMTTTDGIYFNGVRRVKGDSAGPRSAESVLQHPLVEVAVLETARGGILRSGL
                     AWDRSTVSVVLNIANDHLGMEGIETAERLARVKQVVIESVSKDGYGVLNAEDPLVVPM
                     AEVCPGKIIFFGMDSSVPALASHLANGGKAVYTSNDVIVLAEGSEETLLMRTSDIPAT
                     YGGLIPFQVQNVLAAAAACWGAGVSVDSIKLGLRTFQTDEKSAPGRFNLFSIGKARVI
                     IDYGHNPHALRAVQGAVRQMKPRKAIGVVASPGDRRDADIQELAVVAANTFDHIIIRE
                     DEDLRGREPGEVAQLMADTIRRARPSVPLTITLDEREASMQALEMARDGDLVVLFIDH
                     VDEAIALVKSVGKVLSDEHQSSFFRELPRTSNTEQYARIQAIADAAAQESVGSPWKDS
                     GPDGDGAGRPGYTELGGLEETRPQTSAAVDGETRK"
     gene            complement(5006..>5982)
                     /locus_tag="VFH60_09525"
     CDS             complement(5006..>5982)
                     /locus_tag="VFH60_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6314063.1"
                     /translation="DGAFDNYREFLRMQRPSDADSLLAQRIRLYEQVVAEHPKSPQFH
                     VNLAHIYVSSSLDPTMGDQSQSENLVRMAEEEYNKALSIDPDAPFLHHWLGQYHLRIS
                     HDYAKAREEFHQAGQLARKEAIYPAAEADALKVLADEQQTSGQGEAKENYSLALGLYA
                     QAQELDPLGDYHSRIGSVYLAQHDYEKARDEFALATRPNKAAESWSSLGEAYFNERQY
                     EQGIDAFKQATCRDPHNWVYANLLGVTYANKRQFSEARLAFERASALSPTNPIPIAWL
                     AIMDNEQGNPQACEELARAIELAEKPGSGAGKEMVDRWKQEQGYWGCR"
BASE COUNT          971 a         1921 c         1849 g         1241 t
ORIGIN      
        1 agttcaggct ctagcagccc gtagtcaccg tcgttgcgcc tgtagactac ccccacccgc
       61 tcctggtcgg agttacagaa gacgtagaag ttgtggccaa gtagctccat atgctctatg
      121 gcctcttcgg gatccatcgg cttcatgcgg aagcgtttca cccttacgat tgcgccctca
      181 tcgctacccc cgtacttgcc gttgcccgcg aaggcctcgg cggctggctt ggcgggcgcg
      241 gccatctcga tcacgctcgg ctcctcggtg gagacgttca tcgcggcgct cctgccaagc
      301 ccctgcccgc ccttcttgtt gcggctctgg cgggtcttgc gtcgttcaag ctgcttgtcg
      361 aggttggcga gcgcctggtc caccgcggtc agaagctcgt cgtgagccac ttccgagcgc
      421 acaatgttat gtctagtgtg cagggtgact tgcgcgacgt actggaagcg ctgggcgttg
      481 tggcgcgtcg gctgctggga gagcaccacc tcggtggact ccaggtcgtc cacctggcgg
      541 ttgcgcttgg tgaggtgctt gcggatgtgc tcctctatct ttggctccaa cttgaagttc
      601 ttgggtcgga tatttacgga catggagacc tcccgggtgg aaaactgaag acaaagaaaa
      661 ggaccagact acaagcacac cgggccgagt ctaacgttta tagctaactc gggcctctat
      721 gctcatagtg cggtccttcc ggtgcgcgtg gagggattcg aacccccgac gcagggctta
      781 ggacgccctc gctctatccc ctgagctaca cgcgcagtgc agttgagtgc gttcattata
      841 cccgagacgg cgcgcccatt tcaagcagca cccgccgcgc cctcatgtcg tactcgccgc
      901 cggaggagag cacggccacc tccaaaggcc ccaccgagat gggcgtgccg gggaacgtgc
      961 cgtgcgccgt gttcacctgc gcgtgcgagg cgtccacgaa gtagaccacc ccggtgccct
     1021 ttacgatgcc gcgaccgtgg cagtcgagta ataagcaggt attttcgtcg atgcccacgc
     1081 ccagcgccga cgggtgttcg gccaccgagg ctgtgagccg tgggaagcgt cccctcgcgc
     1141 cgaagtgggt atcgatgatg aggtcgtcgc ccgcccagcc gaggccgttc gacatctgca
     1201 ccatgcccat gcgtagctcg ccggtgccgc cgcccgccac caccatcggc ctgcccatag
     1261 cggtcgcgcc cgcgctggtg cccgccacca ccgccccgca cccgtaagcc gccatgatcg
     1321 ccttttcggc atgcgtgcgg tcgagcacct gcgtgagtgc gtactggtcg cccccggtga
     1381 tgaagatgcc ggcggcgcgg tcgagcaggc ccaccgtgtg ttcgtccata gcctcgtcgt
     1441 ggttgcggat taggggcgcg tggatgcggg ccgggtcgta gccgtgccgc gtgtagatgc
     1501 gcacgtactc gttcttgagt atctccgggt ggcgggtggc cgtggtaatg agcacgatgt
     1561 cccgctgctc cgaattccgt tcggcccgct ccacgagctg caggaacagc ttggcgagga
     1621 tacccgtgtg gtccaggcgg tcggcccccc cgattatcat caggtagccc ggcagctccc
     1681 agggggcatc ggccatcgac ggcgcgctgg caagcagtcc cttctgtcgt cgtccgttag
     1741 tagaactcat ttggtccttg atctagatct gcaatcacga atatgagcgc cctccgtacc
     1801 catgcagaat accccatgaa tggcaccggt gttgtctatt tcctcgtttc cccatcaacg
     1861 gccgcgctcg tctgcggcct cgtttcttcc agcccgccaa gctcggtata tccgggccgc
     1921 cccgcgccgt ccccatccgg accgctgtcc ttccagggag agcctacgct ttcctgggcg
     1981 gcggcatccg cgattgcctg gatgcgagcg tattgctcgg tgttggaggt cctgggtagc
     2041 tcgcggaaga agctcgactg gtgctcgtct gaaagcacct tgccgacgct cttcaccagc
     2101 gcgatagcct cgtccacgtg gtcgatgaag agcacgacca ggtcgccatc gcgggccatc
     2161 tccagcgcct gcatggaggc ttcgcgctcg tccagggtga tggtcaaagg cacggagggg
     2221 cgggcgcggc gtatcgtatc ggccatcagt tgcgctactt ctcccggctc gcggcccctc
     2281 aaatcctcgt cctcgcggat gatgatatgg tcgaaggtat tggccgccac gacggctagc
     2341 tcctggatat cggcgtcgcg gcggtcgccg ggggaggcca ccacgcctat cgccttgcgg
     2401 ggcttcatct gccgcacggc accctgcacg gcccggagcg cgtggggatt gtggccgtag
     2461 tcgatgatta ctcgcgcctt gccgatgctg aacaggttga agcggccggg cgcgctcttc
     2521 tcgtcggtct ggaaggtgcg caggcccagc ttgatcgagt ccacgctcac cccggcaccc
     2581 cagcaggcag cggccgccgc gagcacgttt tgcacctgga acggtatcag cccgccgtaa
     2641 gtcgccggga tgtcggaggt gcgcatcaac agcgtttctt ccgagccttc ggccagcact
     2701 atcacgtcgt tgctcgtgta gaccgccttg ccgccgttcg ccaggtgcga ggccagcgcg
     2761 ggcaccgagg agtccatccc gaagaagatg atcttgccgg ggcagacctc ggccatggga
     2821 accaccaggg ggtcctcggc gttcagcacg ccatagccgt ccttgctcac gctctctatc
     2881 accacctgct tcacccgggc cagcctttcc gccgtctcta taccttccat gccgaggtgg
     2941 tcgttggcaa tgttgagcac cacgctgacg gtggagcggt cccacgccag gcccgaacgc
     3001 aggatgccgc cgcgcgccgt ttccagcacg gccacctcca cgagggggtg ttgcagcacc
     3061 gactccgcgc tacgaggtcc ggcagagtcg cccttcacgc ggcgcacacc gttgaagtag
     3121 atgccgtcgg tggtggtcat gcccacgaac ttgcgcatgg tcgagtagat atgggcgatc
     3181 atacgggtca cggtggtctt gccgttggtg cctgtgatgg cgatgagggg cacgcgtgcg
     3241 ggggtgccct ctgggaagag catgtccaca atggcctcac ccaccgggcg cgacctgccc
     3301 tccgaggggt agacgtgcat gcgcaggccg ggggccgcgt tcacctccac gatgccgccg
     3361 ttctggtcct gtagggggcg cttgatgctc tcgcacacca cgtccacgcc cgcgatatcg
     3421 aggccaatcg tgctcgcggc cagcaccgct atcgcctcgt tgtcggggtg gacgatgtcg
     3481 gtcacgtcga tagccgtgcc tccggtggag aggttcgagt tgtcgcgcag aaagaccacc
     3541 tgccccgcgt ccggcacggt ctcccaggtg tggccttgct tgcccagggt gagttccgcc
     3601 gccgcgtcca tcttgatgcg ggtgagggcg gagccgtgtc ccgccccgcg ccgtgggtcc
     3661 tcgttcaccc gcttgaccag ctcgcgcacg ttgctgcgcc cgtcgcccac cacctgcggg
     3721 ggccgccgct gggacgccgc caccagcttg ccgttcacca ccagcagccg gtggtcggtg
     3781 ccgctcagga agcgctccac gattacggcg tcggagtgct gttgggccag ctcaaacgcc
     3841 ttccgcacct gttcctccgt ggtgaggttg acgcttaccg ccttgccctg gttgccgtcg
     3901 aaaggcttga gcaccacagg gccgtcgagg tcctgggcgg cgcgccaggc gtcgtcggcg
     3961 ttgtccacca cctcgccctc cggcacgggc acgcccacct tagccagcag gtccttggtt
     4021 agctccttgt cggaggcgat ttcgacgcct atattggccg tgaaagaagt ctctgcggcc
     4081 tgtatgcgtt tggcgtactt gccgtgcccc agttgcacga gagcggcacg gttgagccgc
     4141 atccagggga tgcgacgctt gcgggccgcg tccacgatgg tctgggtgga ggggccgagc
     4201 atgttgtcct cggcggtgtc gcggatctgc tcgatgcgct ctgccaggtc gaagtcgagc
     4261 ggctgcccct cggccagctt ctcggccatc tctatagcca ggtatgcggc ctgtttggcg
     4321 gcgtcggggt ctttgtagtc caccacgatg cggtactcgc cgtagtgcgc ggtgccccgc
     4381 gtgcggccgg agcccacatc cagcccggcc agcgactgaa gctcgaggat gatgtgctcg
     4441 atgatgtggc ccatgtaggt gcccatgcgt aggcgttcga ggaagccgcc gggctgcccc
     4501 tcggagcagc ggtgctccca gaggctgggt atcgcctcga ccaggcgctc cgtgaagccg
     4561 ggcagcttat ccgagggcaa ctcctcgtac ttgcctatgt cggccaggat ttccagcacc
     4621 ggcctgtagg cccagatgtt ggcgccggac agcaccttta tcttcttaac cttgatctcg
     4681 ctcatacatc gtcctcatag cgggaagcta tattacttgc acgaggtgtg ccgaagcata
     4741 ccgacagtaa agcatgcacc gtcgcaaggc tttcctaatt gtcataccaa ttatcatacc
     4801 aaaagcctgg gagctgtaaa agcttgatgc aaaacgccac acaaggcaag cacataccgg
     4861 tttgcttggc atggcatggc taccttgcca gggtgccgtc actctcggca gggaggcggt
     4921 gggttagccg gtagtagccc tgtcacgtta gccgtgcgat ggagaggcgg ctaacgtgac
     4981 agggctactg ccgacactcg acagtctacc ggcaccccca ataaccttgt tcttgcttcc
     5041 agcgatcaac catctctttg ccagcaccac tccctggctt ttctgccagc tcaatagccc
     5101 tggccaactc ctcacaggct tgtgggttcc cttgttcatt gtccatgatg gccaaccatg
     5161 cgatgggaat tgggttagtg ggtgacaacg cggaggctcg ttcgaaggcc agccgcgcct
     5221 cggagaattg cctcttgttg gcgtaggtga cgcccaacaa gttagcgtag acccagttgt
     5281 gcgggtcgcg gcaagtggct tgcttgaaag catcaattcc ctgctcgtat tgccgctcat
     5341 taaaataggc ctcacctaga ctggaccacg attccgcggc cttattcggc cgggtcgcaa
     5401 gcgcgaactc gtcgcgcgcc ttctcatagt catgttgggc gagatatacg ctgccaatcc
     5461 tggagtggta atcgcccagc gggtccaact cctgcgcctg ggcatatagg cccagggcca
     5521 gcgagtagtt ctcctttgct tcaccctggc cgctcgtctg ctgttcatca gcgagcactt
     5581 tcagcgcatc cgcttcagcg gcaggataga tcgcctcctt ccttgctaat tgtcccgcct
     5641 ggtgaaactc ctcccgcgcc ttcgcatagt cgtggctgat tcttaggtgg tactgtccta
     5701 accagtggtg caggaagggg gcgtcaggat cgattgatag cgccttgttg tactcttctt
     5761 ctgccatgcg aacaaggttc tcagactgag attgatcgcc catcgtaggg tccagactgg
     5821 aactaacata gatatgggcg agattaacgt gaaattgagg gcttttcggg tgttccgcga
     5881 ccacttgttc atagaggcgt atacgctgcg ccaacaaaga gtctgcgtcg gagggtcgct
     5941 gcattcttag gaactcccgg taattgtcaa atgccccgtc at
//
LOCUS       DASRWZ010000605         1953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_940003, whole genome shotgun sequence.
ACCESSION   DASRWZ010000605 DASRWZ010000000
VERSION     DASRWZ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940003"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFH60_09530"
     CDS             complement(<1..184)
                     /locus_tag="VFH60_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET6314064.1"
                     /translation="MDNIPDLYNNPYPSTRSVTIAREGVVATSQPLAVQAGISMLQRG
                     GNAVDAALAAAIALTVV"
     gene            517..1842
                     /gene="asnS"
                     /locus_tag="VFH60_09535"
     CDS             517..1842
                     /gene="asnS"
                     /locus_tag="VFH60_09535"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET6314065.1"
                     /translation="MQTEETTTRQTPVVYIADLKDHDGQVVTVRGWLMHHRDKKKLQF
                     LVLRDGTGTVQAVAFIDDVPPESWEKIGRLTQESSVMVTGTVRKDERAPGGYEISVKD
                     IDVFQVAGADYPIANKEHGVGFLMEHRHLWLRSNKQRAVLRIRAEVVKAVRDYLDEHG
                     YVLVDTPILTPAACEGTSTLFETDYFGTPAYLAQSGQLYNEADIFAFGKVYCFGPTFR
                     AEKSKTRRHLMEFWMVEPEAAWMELDGLIELEQSFVSYIVRTVLEKRRAELELLERDT
                     SKLENVLEPFPRITYDRALEILKENGLEVQWGDDFGGDEETVLASQYDRPLFITHYPA
                     HAKAFYMQPDPNRPEVVLCADLIAPEGYGEICGGSQRIHDAALLEQRITEHGLPREAF
                     EWYLELRKYGSVPHSGFGMGIERLVAWICGVPHLRETIPFPRMLDKIYP"
BASE COUNT          372 a          624 c          595 g          362 t
ORIGIN      
        1 ccaccacggt gagggcgata gccgccgcca gggcagcgtc cacagcgttg ccgccccgct
       61 gcaacatgga gataccggcc tgcaccgcca gcggctggct ggtagccaca acgccctcgc
      121 gggcaatcgt cacgctccgg gtcgagggat aggggttgtt gtaaaggtcg gggatgttgt
      181 ccacggcggc acctcctggg gtttgtctgg ctcgaattat aaccgaggcg cgaaaatcag
      241 tcagcaggag gggacgagac gaaaccgcca agacgccaag ataataagga gggtgttcaa
      301 agcaaaaggg ccaggataca tgctgttgta ccccggtcct ttttgtaccg tctaaacctt
      361 acttctcttt gcgcgtcctt tgcgttcttt gcgtctttgc gttttcgtct attggcgcgc
      421 tcttgggtat tggcggtttc gtctcggcct ctgtttgccc ccaggcgccc gctgtgatat
      481 gattcgtggg ttagctcaga aggagacacc cacaacatgc agacagaaga gactactacg
      541 cgacagaccc ctgtcgtcta tatcgcggac ctgaaggacc atgacggcca ggtcgttacc
      601 gtgcggggct ggttgatgca ccaccgcgac aagaaaaagc tgcaattcct ggtgctgcgc
      661 gacggcacgg gcaccgtgca ggccgtggct ttcatcgatg acgtgccccc ggagtcctgg
      721 gagaagatcg gcaggctcac ccaggaatcg tcggtcatgg tcaccggcac agtgcgaaag
      781 gacgagcggg ctcccggcgg ctacgaaatc tcggtgaagg acatcgacgt gttccaggtg
      841 gcgggggccg attaccccat cgcgaacaaa gagcatggcg tgggcttcct catggagcac
      901 cggcacctgt ggctgcgctc caacaagcag cgtgccgtcc tgcgcatccg cgcggaggtc
      961 gtcaaggcgg tgcgcgatta cctggacgag catggctacg tgctggtgga cacgcccatc
     1021 ctcacccccg ccgcctgcga aggcacctcc accctgttcg agaccgacta cttcggcacc
     1081 cccgcctacc tggcgcagag cggccagctc tacaacgagg ccgacatctt cgcgttcggc
     1141 aaggtctact gcttcggccc caccttccgc gccgagaaga gcaagacccg ccgccacctt
     1201 atggagttct ggatggtgga gcctgaggcc gcctggatgg agcttgacgg cctcatagag
     1261 cttgagcaga gcttcgtcag ctacatcgtg cgcaccgtgc ttgagaagcg ccgcgccgag
     1321 ctagagctgt tggagcggga cacctctaag ctggagaatg tgctcgagcc tttcccccgc
     1381 atcacctacg accgggcgct cgaaatcctc aaggagaacg gcctcgaggt gcaatggggc
     1441 gacgacttcg gcggcgacga ggagacggtg ctcgcctccc agtacgaccg ccccctattc
     1501 atcacccact accccgccca cgccaaggcc ttttacatgc agcccgaccc caatcgcccc
     1561 gaggtggtgc tgtgcgccga cctgattgcc cctgagggct acggcgaaat ctgtggtggc
     1621 tcccagcgca tccacgacgc cgccctcctt gagcagcgca tcacggagca cggcctgccg
     1681 agggaagcct tcgagtggta cctcgaattg cgcaagtacg gctcggtgcc ccactccggc
     1741 ttcgggatgg gcatcgagcg cctggtggcc tggatttgcg gcgtcccgca cctgcgcgag
     1801 acgatcccct tccccaggat gctcgacaaa atttacccgt aaggtgtgag aagtacaacc
     1861 tggtgcggcg ggctggcact cgctctctag cgtgcgccac cacgaccaac atcgtatcac
     1921 gggaccaggc caccctttgt cattctgagt ggt
//
LOCUS       DASRWZ010000606         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1008188, whole genome shotgun sequence.
ACCESSION   DASRWZ010000606 DASRWZ010000000
VERSION     DASRWZ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008188"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="VFH60_09540"
     CDS             <1..1407
                     /locus_tag="VFH60_09540"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6314066.1"
                     /translation="EYIPGQRQEAWGTVGFAPPEIGGICEQPPSMDLFGITSTLAAIL
                     GVDVNQQQIGAPPPSAWPVSQEIYDFVERGRDPDPRVRFQTVEELFDQLEGINRFIQG
                     QERARVAGSPQMQANRSTSTLAATLVPVQSTLFTGKLLTGSTGKIGVLPALSATDPAS
                     QMLQPARDLMAAGRYGEALPQIEATLKTHPASIDGHLLRAASLNNMGQAEEARQALEQ
                     AGRLSTPATRWRTLLVQAQAAEAVQDYNRAEQLYAELIKLVPGEVLPKQGLADIYRET
                     GRYAPAADMYQRVVSADPANADAVLGLADSLVALDRTDSAIRVLDAVNENALRYVDAQ
                     LRLIELFLVRIDGYMTKQAARFYQGKPGDPDTQALLHDLNLAGHAIARLNDRTESRHF
                     YRLQAEWWYAAYRMAKAKRLTPNMEWPDGKPHKADDPQEIGQACREAYRRYLQRAPDA
                     PDREDVLARIYFDVAEWQ"
     gene            1525..>2491
                     /locus_tag="VFH60_09545"
     CDS             1525..>2491
                     /locus_tag="VFH60_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET6314067.1"
                     /translation="MVKFRVTPHPGEAQALQGHNLVRTLYEVAADASVGGGGGSSVPV
                     FLGVVMDQSGSMEGEKLYSAKEALLKLLREVPPSDNVIVDITLFSDEADPFTPAMSGR
                     QISQNFDDLTRRIRNISAGGATSLGSGLRIALRDSARYGEYERRVLMITDGKQEGTLP
                     ISDAYNAANELAQAGIRVDAWGVGNAWEADQLRTIAHSTGGEADVIPNARQLADAVSS
                     LFTDVQSTKASNVRLVLNTPVGTQIRSVRQVYPSVQDRAAQQINEQKWIIPIGALTQE
                     EPKFVIELETTPRGTNIPFRILVPTVLYNVGSEELTDELDRSAWFF"
BASE COUNT          512 a          806 c          772 g          401 t
ORIGIN      
        1 gagtacatac cgggccagag gcaggaagcg tggggcacgg tcggcttcgc cccgcctgag
       61 atcggcggca tatgcgagca gcctccctca atggacctct tcggcatcac cagcacgctg
      121 gccgcgatcc tgggcgtgga cgtgaaccag cagcaaatcg gcgcgccccc ccccagcgcg
      181 tggcccgtct cccaggagat ttacgacttc gtagagcgcg ggcgcgaccc cgacccccgc
      241 gtgcgcttcc agacggtgga agagttattc gaccagttgg agggcatcaa ccgcttcatc
      301 cagggtcagg agcgcgccag ggtagcgggc agcccccaga tgcaggcgaa tcgctccaca
      361 tcaaccctgg ccgccacgct cgtgccggtt cagagtaccc tattcacggg caaattgctc
      421 acgggcagca ccggcaagat aggcgtattg cctgccctca gcgcgaccga ccctgcctcg
      481 caaatgctcc aacccgccag ggaccttatg gccgccgggc gctacggcga ggcccttccc
      541 cagatagaag ccaccctcaa gacccacccc gccagcatag acggccacct gctccgcgcc
      601 gcctctctga acaacatggg ccaggccgag gaagccaggc aggcacttga gcaggccgga
      661 cgcctcagca cgcctgccac gcgctggcgc accctgttgg tgcaggccca ggcggcagag
      721 gctgtgcaag attacaacag ggcggagcag ctttacgccg agctaatcaa gctggtgccc
      781 ggagaggtgc tgcccaagca ggggctggcc gacatctaca gggagacggg ccgctacgct
      841 cctgccgccg atatgtacca gcgtgtggtg tccgccgacc ccgcaaatgc cgacgcggtg
      901 ctcggcctgg ccgacagcct ggtggcgctc gaccgcaccg actccgctat ccgcgtgctg
      961 gacgccgtga acgagaatgc cctgcgctac gtggatgccc aactgcgtct gatagagctt
     1021 ttcctggtgc gtattgacgg ctatatgact aagcaggcgg cgcgtttcta ccagggcaag
     1081 ccgggcgacc cggacacgca agccctcctt cacgatctca acctggccgg gcacgctatc
     1141 gcacggctga atgacaggac ggagtcgcgc cacttttacc gcctacaggc cgagtggtgg
     1201 tatgcggcct atcgcatggc aaaagcgaag cgcctgacac ccaatatgga gtggcccgat
     1261 ggcaagcccc acaaagcaga cgacccacag gagatcggcc aggcgtgccg cgaagcctac
     1321 cgccgctacc tccaacgcgc ccctgacgcc cccgaccgag aggacgtgct agcgcgcata
     1381 tactttgatg tcgctgaatg gcaatagacc gatttggaat ttcggatttc gaattgcgaa
     1441 tttcatactc acatactaaa tggcgcaagg aaaccaaatt cgaaatccga aattcgcaaa
     1501 ttccaaatta agaagggggt ttacatggtc aagtttcgcg taacgccgca ccctggcgag
     1561 gcgcaggcat tgcaggggca caacctcgtg cgtaccttat atgaggtagc cgccgatgct
     1621 tccgtaggtg gagggggtgg ttcctcggtg ccggtcttcc tgggagtggt catggaccag
     1681 tcggggagca tggaggggga gaagctctac tcggcgaaag aggctctgct caagctgctg
     1741 cgcgaggtgc cgcccagcga caatgtaata gttgacataa ctctgttcag cgatgaggcc
     1801 gacccattca cgccagccat gtcgggtcgc cagatctccc agaactttga cgacctgacc
     1861 cgccgcatcc gtaacatcag cgcgggcggg gccacttcgc tggggagcgg cctgcggata
     1921 gcgttgcgcg attcggcgcg ctatggagag tatgagcggc gcgtgctgat gattaccgac
     1981 ggtaagcagg agggcactct ccccataagc gacgcctaca acgccgcgaa cgaattggcg
     2041 caggcgggca tcagggtgga cgcctggggc gtgggcaacg cctgggaagc cgaccagctt
     2101 cgcactatcg cgcacagcac gggcggcgag gccgacgtga taccgaacgc ccggcaactg
     2161 gccgacgcgg tcagctcgct cttcaccgac gtgcagagca cgaaggcgag caacgtgcgc
     2221 ctggtgctca acacgcccgt cggcacccag ataaggagcg tgcgccaggt ctacccgtcg
     2281 gtgcaggacc gcgccgcgca gcagatcaac gagcagaagt ggatcatccc cataggcgcg
     2341 ctcacccagg aagagccgaa gttcgtcatc gagcttgaga ccaccccgcg cggtactaac
     2401 atacccttcc gcatcctcgt gcccactgtt ctgtacaatg tggggagcga ggagcttacg
     2461 gacgagcttg accgctcggc ctggttcttc g
//
LOCUS       DASRWZ010000607         6877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_665834, whole genome shotgun sequence.
ACCESSION   DASRWZ010000607 DASRWZ010000000
VERSION     DASRWZ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665834"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1108)
                     /locus_tag="VFH60_09550"
     CDS             complement(38..1108)
                     /locus_tag="VFH60_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6314068.1"
                     /translation="MTAGTDNHQPLRLGLVGCGRVVEKYHLPALKRSRAWTLAAACDP
                     SPERRRWVQSAAHGVPTFERPGEMFGIVLLDAVLVATPPTTHFAVSMQALEKGLHVLV
                     EKPMSLSAEEAHLMLGTARQASKLLLVGFNRRFHSTYTQLRAKLGTPNMAGVSTVSSN
                     FIFDAGAWGAHLGDEAAGGGVLDDVVCHQVDLLCWLLGRRAAKVRATDATIGSKGARL
                     IEYELDFCDGLVARCAAGHGREYQEEISIGLDDKTVIARPGAPLSAGTAPGITRLWDS
                     IGATTARLVSKASGRPSPTVESFGGQLKAFAAGIRAGAPHEHSADAWSGLYSVQVVEA
                     ARESIRSGGRWQQIASTMETDV"
     gene            complement(1105..2310)
                     /locus_tag="VFH60_09555"
     CDS             complement(1105..2310)
                     /locus_tag="VFH60_09555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6314069.1"
                     /translation="MPRLKIAMLAACPFPWPRGTPARVLRLSEALCERGHEVHVVTYH
                     LGDRSIRLPFKVHRTPRLPTYSRTAPGPSLQKLALLDPLLALELRRVLRQERIDVIHA
                     HHYEGLLVSAWAQMGAARLPVVYDAHTTLESELPSYGPPLAGALKRRVGREIDRWLPG
                     RAGHIVAVTEQIRERLVRVAGVPSKRVTVGPNGVELERLAQARSAGRRGGDSAGRAYT
                     LGYAGNLAPYQGIDILLLAAKKVLDVRRDVRLVVATGSPTAKWEAEARRLGIGAHVEF
                     ANPNLDALPGVLATFDVALNPRPHCDGIPSKLLNYMAAGKPIVSFQGSARGVREGALL
                     AVPGEDAEAFAAAVLRLLDEPDLARCLGGRSLRAATEDHSWARTAEIVEGVYMRLLGR
                     IDKAEGIVS"
     gene            complement(2258..3595)
                     /locus_tag="VFH60_09560"
     CDS             complement(2258..3595)
                     /locus_tag="VFH60_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET6314070.1"
                     /translation="MKANNLDWRKVGRDLRLTFFSHFFLKFVGYLVLVVLARYVEKGQ
                     VGEFFFALSLASLWVLCTELGTGNYLMRAVACEPGEATEEFSRVLSLRLALAVPYFLA
                     LNGLVALVRPEMALTAALVSIYVFLDQLYGSFAALFLGLGRADYSVAARVGSGTLLIS
                     SVFVAANLGWGLWPILGCYILSSAVLVGSAVWLVRGKIGPLRLRWDLADFARLAASSM
                     PFFAATALVMVHANVDAVSLGLLQPYAVVAAYAVGFKLLDVLRYMTRPVTAILYPLCS
                     RMSHQGDWAGIESLLKRLLVGGAVLGVGVSAGAIVAADFVVESVLGNKYQETPGLVKV
                     LFLSVAPLYVGTLSAMFANALHLERRAARVMLPIVAANVALSVAGVVVAGAMGAAWTT
                     VLSESIIAAAVTLLVLKELRSRRASPRAAHYPEERAYATSEDSHAGSLPLPLA"
     gene            complement(3651..4730)
                     /locus_tag="VFH60_09565"
     CDS             complement(3651..4730)
                     /locus_tag="VFH60_09565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6314071.1"
                     /translation="MSRLDARAPVSLVIVNYNGEEYLRGALRAVREAGDDFGEVVLVD
                     NASTDASLRIASEEYPAARSVRLDRNCGPARARNVGLMAARHERVLFIDCDVTLTAGC
                     AQTLASALDACPRAVAAMPTVVYNHDREIAQYSGADSHFLGLMSLRDADVPVNELCGR
                     APQSLGSLVTCCFLLDRERWGGQPPFDENFFSLYEDHDFGLRCRVLGHSILSVTGARV
                     YHGAGTEGLSIRRTGRYTSRRVFLLVRNRWQVVLKNYQGRTLVTLLPVLLLYEMFQFG
                     AVLKKGWAREWLQALRWLVRHRQAIMRKRRAIQAARRLPDRDLLSGGPLPFTGYVTTS
                     RLERLGKRTLDAVANAYWNVARGAL"
     gene            complement(4727..6277)
                     /locus_tag="VFH60_09570"
     CDS             complement(4727..6277)
                     /locus_tag="VFH60_09570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6314072.1"
                     /translation="MRAEAARGGAARSILPVAGRLGVGVRSVPAGALLGAVLALGATL
                     RLYDLGSESYWLDEVIMLRAAEGDVGSILGGTRPPVYVLLTHFWIQFFGTSEASTRLL
                     PALFGTLSVLLTYVVGRELFGKRVGLVAALLMSLSAFQVYYSQEIRYYSLFVMCALLS
                     YLLMLRAHKTGKPIYFALYALAGIVMFYSHSYAVFVFAAQNLYFALGWKRLRLLLGYW
                     LISQAVIAMAILPALMLPADRVITGSAAVMKWIPDPPLWQPFFDVMGYVVPIYSSSKV
                     ALAIVPLLALIALAVVAYAKGREKWWAAARHTAAGIKHGVAGERDKLLLLGCWLLCPI
                     FIPYLMSKVFGPMYLSRYTISATPAFYILAALVILAGRKLMPAYISLALVVVLVAPGM
                     IEYYARPVKDEWREAAAFVRERSRPGDVLMAEYSDNFVLSWYDKDDLTRCVTDPSLAA
                     GITRDERMRREVIAWATGSPRFWLATSAAEKDPYLQFFSSEVPGLGLVEHRDFAGLST
                     YLFSRSGP"
     gene            complement(6274..>6877)
                     /locus_tag="VFH60_09575"
     CDS             complement(6274..>6877)
                     /locus_tag="VFH60_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314073.1"
                     /translation="ADIPLLLDKVRQGYDLVSGWRRHRRDPFLTRKLPSRLANRLIGL
                     VTGVRLHDYGCSLKAYRSEVVKELRLYGEMHRFIPALASRMGARITEVPVSHYPRLYG
                     RSKYNMGRTFRVIPDVLAVKLLLSDASSSARPFRIFREWATRGHCRAMDRPAFVISER
                     LRHDTDPQGKLEAEAPVLLAGSARSPHAAAFTSLVRESMQ"
BASE COUNT         1251 a         2388 c         2105 g         1133 t
ORIGIN      
        1 gcgtcgatgc cgatagcgag tacgggtacc ttgttcgtca tacgtcagtc tccattgtcg
       61 aagctatttg ctgccagcgc ccacccgacc ggatactctc ccgcgccgcc tccaccacct
      121 gcaccgagta caggccgctc cacgcgtcgg cgctatgttc gtgaggcgca cccgccctta
      181 taccagcggc gaacgcctta agctgccctc cgaagctctc caccgttggg ctgggtctgc
      241 cgcttgcttt gctgaccagc cgcgccgtgg tagcgccaat cgagtcccat aggcgggtaa
      301 tgccgggcgc ggtccccgcc gaaagtggcg cgccggggcg tgctatcacc gtcttgtcgt
      361 ccagtcctat gctaatctct tcttgatact ccctgccgtg ccccgccgca catcgcgcca
      421 cgaggccgtc gcagaagtcc aactcgtact ctatcaggcg tgctcctttg ctgccaatgg
      481 ttgcatccgt ggcacgcacc ttcgccgccc gcctcccaag cagccagcag agcaggtcca
      541 cctggtggca gacaacgtca tccagcactc ccccgcccgc cgcttcatcg cccaggtgcg
      601 cgccccatgc gcctgcgtcg aagatgaaat tggaacttac ggtgcttaca cccgccatat
      661 taggcgtgcc aagtttcgcg cgcaactgtg tgtaagtgga gtgaaagcgg cggttgaagc
      721 cgaccaacaa aagcttactt gcttgcctgg cagtccctag catgaggtgc gcctcctccg
      781 cgctcagcga catcggcttt tcgacgagta catgcagtcc cttctccagc gcctgcatgc
      841 tcacggcaaa gtgggtcgtt gggggcgtgg cgacaagcac cgcgtccaga agcacaatcc
      901 cgaacatctc gccgggccgc tcgaacgtgg gtacgccatg cgccgcgctt tgcacccaac
      961 ggcgacgctc cggggagggg tcgcaggccg ccgcgagcgt ccaggcccgg ctcctcttga
     1021 gcgcgggcag gtggtatttt tcaactaccc tgccgcagcc cacgaggccg agcctgagcg
     1081 gttggtggtt gtcagttcca gccgtcacga aacgattccc tctgctttgt caatacgccc
     1141 cagcagccgc atgtagactc cctcaactat ttccgccgtt cgggcccagg agtggtcttc
     1201 cgtcgccgct cgaagtgagc gtccgcccag gcagcgcgca aggtccggct cgtcaagcaa
     1261 gcggagcacg gcggcggcga aggcttcagc atcttctccg ggcacagcga gcagcgcacc
     1321 ctcgcggacc cccctggccg agccctggaa agacacaata ggcttgcctg ccgccatgta
     1381 gttgagcagc ttggatggta taccgtcgca gtgcgggcgc gggttgaggg ccacgtcgaa
     1441 ggtggcgagc acgcctggca gcgcgtctaa gttggggttg gcgaactcta cgtgcgcccc
     1501 tatgcccaac ctcctggctt cggcctccca cttcgcggtg ggcgaacccg tggctaccac
     1561 caggcgcacg tcccgcctca cgtccagcac ctttttggcg gcaagcagca ggatgtctat
     1621 cccctggtaa ggtgccagat tgcccgcgta ccctagcgtg tacgcccgcc ccgcgctatc
     1681 tcccccgcgc cgcccggccg agcgcgcttg cgccaaccgt tccaactcca ccccattggg
     1741 accgaccgtc actcgctttg aaggcacccc cgcaactcgc accagcctct ccctgatttg
     1801 ttccgtcact gccacgatgt gccccgcgcg gccgggcagc cacctgtcta tctcgcgtcc
     1861 taccctcctc tttagcgcac ccgctagcgg cggcccgtaa gagggcagtt ccgactccag
     1921 cgtggtgtgg gcgtcataca caacgggcag gcgcgcggcc cccatctggg cccacgccga
     1981 caccagcagc ccctcgtagt ggtgggcgtg tatcacgtcg atgcgctcct gccgcaatac
     2041 tcgccgtagc tccagcgcca gcagcgggtc gagcagggcc agcttctgca gggaggggcc
     2101 gggcgcggtc ctgctgtatg tcggcaggcg tggggtgcgg tggactttga agggcagtct
     2161 gatgctcctg tcgcccaggt ggtaggtgac tacgtgcacc tcgtgccccc gctcgcacag
     2221 cgcctccgac agccgcaaca ccctggcggg cgtgcctcta ggccagggga aggggcaggc
     2281 tgccagcatg gctatcttca gacgtggcat aggcccgttc ctccgggtag tgggcggcac
     2341 gcggagaggc acggcgcgac cgcagttcct tcaacaccag cagggtgacc gccgccgcga
     2401 ttatagactc cgacagcacc gttgtccagg ccgcccccat cgctcccgcc acgacgaccc
     2461 ccgccaccga cagcgccacg tttgccgcca cgatggggag catgaccctg gcagcccgcc
     2521 tctcaaggtg cagcgcgttg gcgaacatcg cgctcagggt gcccacgtag aggggggcca
     2581 cgctcaagaa gagcaccttc accagccccg gcgtctcctg gtacttgttc cccagcacgc
     2641 tctccaccac gaagtctgcc gccacgatag cccccgccga caccccaacg cccagcaccg
     2701 cgccgccaac cagcagccgc ttgagcagcg attcgatgcc tgcccaatcg ccctggtgcg
     2761 acatgcgcga gcagaggggg tatagtatgg ccgtcacggg ccgcgtcatg tagcgaagca
     2821 cgtccaacag cttgaacccc accgcgtagg cggccaccac ggcgtagggt tgcagcaggc
     2881 ccaggctcac cgcatccacg ttggcgtgca ccatgaccag ggccgtcgcg gcgaagaagg
     2941 gcatagagga ggctgccaac ctagcgaagt ccgcgaggtc ccaccgcagc cgcaaggggc
     3001 ctattttgcc cctcacgagc cagaccgcgc tccccaccag cacggcgctc gacaggatgt
     3061 agcagccgag gatgggccac aatccccagc cgaggttggc cgccacgaac acggagctta
     3121 tcagcagcgt gccggagccg acccgcgccg ccacgctgta gtcggcccgc cccagcccca
     3181 ggaacaacgc cgcgaagctc ccgtacaact ggtcgaggaa cacgtatatc gacacgaggg
     3241 cggcggtgag ggccatttcg ggccgcacca gggccaccag gccgttcagc gccaggaagt
     3301 aggggacggc aagggccagg cgcagcgaca gcacccgcga aaactcctcc gttgcctccc
     3361 ccggctcgca ggccaccgcc cgcatgaggt agttgccggt gcctagctcg gtgcatagca
     3421 cccacaggct ggccagggac agcgcgaaga agaactcccc gacctgcccc ttctcgacgt
     3481 agcgggcgag cactaccagc acgagatagc ccacgaactt gaggaagaag tgcgagaaga
     3541 aagtcagccg caggtcgcgc cccaccttcc gccagtccaa gttgttggct ttcacgcctg
     3601 cgccctttca accgccgggc gtatctgctt tgtcgaatag agtgccacct tcacaacgca
     3661 cccctcgcaa cgttccagta ggcattcgcc acagcgtcca gcgtgcgctt gccaagcctc
     3721 tccaggcggc tggtggtaac gtagccggtg aagggcaggg ggccgcccga caatagatcc
     3781 cggtccggca gccgtcttgc cgcctgtatc gcccgccgct tgcgcataat ggcctgcctg
     3841 tgcctcacca gccagcgtag cgcctgcagc cactcccttg cccacccctt cttgagcacg
     3901 gccccaaact ggaacatctc atatagtagc agcaccggca gcagtgttac caacgtgcgc
     3961 ccctggtagt tcttgaggac cacctgccag cgattcctga cgagcaggaa caccctcctg
     4021 gacgtgtacc gccccgttcg cctgatcgac aggccctcgg tgcccgcgcc atgatagacc
     4081 cgcgcccctg tcaccgatag tatgctgtgc cccagcaccc ggcaacgcag gccgaaatcg
     4141 tggtcctcgt acaggctgaa gaagttctca tcgaaggggg gttggccgcc ccaacgctcg
     4201 cggtccagca gaaagcaaca ggtgaccagc gagcctaacg actgcggcgc tcttccacat
     4261 agctcgttta ccggcacgtc cgcgtcccgc aacgacatga ggccgagaaa gtggctgtcc
     4321 gctccgctgt actgcgctat ctcccggtcg tggttgtaga cgacggtggg catggcggct
     4381 accgcgcggg ggcatgcgtc cagggccgaa gccagcgttt gggcgcagcc tgcggtgagg
     4441 gtcacgtcgc agtcgatgaa cagcacccgc tcgtgccggg ccgccatgag gcccacgttg
     4501 cgcgccctgg caggtccgca gttcctgtcc agccggacgc ttcgcgcagc cgggtactcc
     4561 tcgctcgcga tgcgcaggct ggcgtcggtg gaggcgttgt ccaccaggac gacctcccca
     4621 aaatcgtcgc ccgcctcgcg caccgcccgc aaggcaccgc gcaggtactc ctccccgttg
     4681 tagttcacaa tcacgaggct cacaggcgcg cgggcatcga ggcggctcac ggcccgctcc
     4741 ggctgaacag gtacgttgac agccccgcga agtcgcgatg ctcgaccagg cccaggccgg
     4801 gcacctcgct tgagaagaat tgcaagtagg ggtccttttc ggcggcggag gtagcgagcc
     4861 agaaccgggg gctgcctgtg gcccaggcga ttacctcccg gcgcatgcgc tcgtcgcggg
     4921 tgatgcctgc ggcgagactt gggtcggtga cgcaccgggt cagatcatct ttgtcgtacc
     4981 aggagagcac gaagttgtcg ctgtactcgg ccatgagcac gtcgccgggc ctgcttctct
     5041 cccgcacgaa tgcggcagcc tcccgccact cgtccttcac gggccgtgcg tagtactcaa
     5101 tcatgccggg tgcgaccaac accacgacga gggccagcga tatgtaagcg ggcatgagct
     5161 tgcgcccggc caatatgaca agggccgcca gtatgtaaaa ggctggggtg gcgcttatgg
     5221 tgtagcggct caggtacatc gggccgaata ccttcgacat gagatagggg atgaatatcg
     5281 ggcacagcag ccagcagccc aatagcaaca gtttgtcccg ctctcccgca accccgtgct
     5341 tgatacccgc cgccgtatgt cgcgccgctg cccaccactt ctcgcgcccc ttcgcatagg
     5401 ctaccaccgc cagggctatc agggcgagca aggggacaat cgccagggcc accttagagc
     5461 tactgtagat gggcactaca tatcccataa cgtcgaagaa aggctgccac agaggcgggt
     5521 cgggtatcca cttcatcacg gccgcgctgc cggtaatcac cctgtcggcg ggcaacatga
     5581 gggcgggcaa tatcgccatt gcgatcaccg cctgcgagat gagccagtag cccagcagta
     5641 gcctcagccg cttccagcca agcgcgaagt aaaggttctg cgccgcgaac acgaacacgg
     5701 cgtagctgtg gctgtagaac atcactatcc cggcgagggc gtacagcgcg aagtagatgg
     5761 gcttcccggt cttgtgggcg cgcaacataa ggaggtacga cagcagggcg cacatgacga
     5821 agaggctgta gtacctgatc tcctgggagt aatacacctg gaacgccgag agggacatca
     5881 gcagggcggc caccaggccc actcgcttgc cgaacaattc gcgtcctacc acataggtga
     5941 ggaggaccga cagggtgccg aacagggcgg gcagcaatct tgtggaggct tccgaggtgc
     6001 cgaagaattg tatccaaaag tgggtgagca gcacgtaaac ggggggtcgc gttccaccga
     6061 ggatggaccc cacgtcgccc tctgcggccc gcaacatgat aacctcgtcc agccagtagc
     6121 tctcgctgcc caggtcgtac aacctcagtg tagcgcccag ggcgagcacc gcccccagca
     6181 aggccccggc gggcacgcta cgcacaccca cacccagccg cccggcgacg gggaggatcg
     6241 agcgcgccgc cccgcctcgt gctgcctcgg ccctcactgc atgctctctc ggaccagcga
     6301 cgtgaaagcg gcggcgtgcg ggcttcgggc gctcccggcc aggagtaccg gggcctcggc
     6361 ctctaacttg ccttgtgggt ccgtatcgtg ccgcaaccgc tcgcttatca cgaatgcagg
     6421 ccggtccata gcccggcagt ggcccctggt tgcccactcc cggaagatgc ggaaaggccg
     6481 ggcagacgag gaggcgtcgg agaggagtaa tttcacggcg agcacgtcgg gtatgaccct
     6541 gaaggtgcgg cccatgttgt acttggagcg gccgtagagg cgtgggtagt ggcttacggg
     6601 tacctcggtg atgcgcgcgc ccatgcggct tgcgagtgcc gggatgaagc ggtgcatctc
     6661 cccgtagagc cttagctcct tcaccacctc gctcctgtaa gccttgagcg agcagccgta
     6721 atcgtgcagc cgcacccccg taaccaaccc gatcagccgg ttagccaggc gcgagggcaa
     6781 tttcctggtg aggaaggggt cgcgacgatg ccgccgccaa ccgctcacca ggtcgtagcc
     6841 ttgccgcacc ttatcgagca ggaggggtat atcggcg
//
LOCUS       DASRWZ010000608         3965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_940069, whole genome shotgun sequence.
ACCESSION   DASRWZ010000608 DASRWZ010000000
VERSION     DASRWZ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940069"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VFH60_09580"
     CDS             <1..1145
                     /locus_tag="VFH60_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6314074.1"
                     /translation="LAGGFQGPTSPSWVPVTLTAIYMLLAALLVFWALYIYLDWKDDW
                     LALTTRRLIMMDKTLVFHETRREVPVLKIQNIVAEYPRLLSMYLDFGDLSVDTAGVGL
                     MVFKDLPHPRQMREVIFLQQSALTASQAPPEDRRKAAMRNLLQGASPNSHDRPTPPNG
                     QPQVPGRGETQLIDYSSVSGIGLLGRFFPLAPQRNAAGVTWHKHWIYLVRGLLWPALL
                     YGIALVAWFAMRVTGASAALNPAEDVLAWVAVLLLPVCLVWAVWRWEDWRNDLYRLDA
                     ERIYHVESLPFGLREESTETLVTRITDVSYSLPGLLAHVLNFGNVIIKTPGESSEIVF
                     QGIPRPREVQGEIMARVDEHRLKDQAGIDGEIEAWIKAYDDVKRGK"
     gene            1305..3890
                     /locus_tag="VFH60_09585"
     CDS             1305..3890
                     /locus_tag="VFH60_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314075.1"
                     /translation="MRAFRAVLPILVLLGLMLPGIVPVQAAPPAASPARVASATFQTQ
                     AGVRLVTTAGFGEDGSYAMGAWFPVRVMLDNPAGAGDMRVRVEVDLSGGSGGSGSSSV
                     GTYARDVDLPSPSRKQVTLYTFAGNFTHTVRVRLLQGEKVIASANVSVDPQEPVNGAI
                     VGVVSSDASLLNVYEGEEVGNPEQPPSSMQFGGPMPPTQEAPRARIAHIQPDDIPPLT
                     AGLNSLAALVVDDVDTGALSQEQREAVASWVGRGGTLVVSARSGGANTLAGFADLLPV
                     SASGTRTLSSLKGIADLVGTPLEASGQVIVPDAQLRADLVGAPRTLAAQDGVPLVAIR
                     DLGRGQVVYLAVSPGADPLKGWDGLVPLLKRVLAEHPVTLAYGGARAGGYGPYNQLFS
                     TYGTIFDLPGLDLPEPLLLAAFMLIYIVLVGPVNYVILKRMRRAELAWVTIPVMVAVF
                     AALAYFIGLQSKGGELLTIRGNVVHTTPGLSTAETQQYLGVFSPIRRTYRLEIGSDAT
                     ASEIDPYGYNYSGGGSGGKLPTVLASGSTTTIENVNVNTWSLRSFMAESTVRVESPLE
                     ADLRLGDNLIEGTVRNRGEVGLEDVVLVRGQAIQYIGFIGPGNQTPVKLNIAHAPFHT
                     TSPVPLLPLPPGVEDPSQSGGYYGGGQDSNQQRDYNRRVQMLSTALEPLLVNEPPSDF
                     TVLAVAWGPSPPSEFRVLDRTTRTEDLNVWTSRLQVAGGDGGQGRITQGTLPAMQYVP
                     DDNPAWKPFDGSEVSLNPFTTVQFRLPQGTQPGSLTLEYEARTSLAGNPLEVLAFNVR
                     TGAWDRLAEISGGQSQQTSVPINNPLDYTGPGGDVTLRIAPTAGSTATRIAFDTFTLS
                     LNDTQ"
BASE COUNT          691 a         1310 c         1309 g          655 t
ORIGIN      
        1 tgctcgccgg ggggttccag ggaccgacct caccctcctg ggtgcccgtg acgctcaccg
       61 ctatctatat gctgctggcc gcgctgctgg tcttctgggc gctctatatc tacctcgatt
      121 ggaaggacga ctggctggcc ctcactactc gccggcttat catgatggac aagacgctcg
      181 tcttccacga gacgcgccgg gaggtacccg tgctcaagat acagaacatc gtggccgagt
      241 acccgcgcct gttgagcatg tacctcgatt ttggcgacct gagcgtggac acggcgggcg
      301 tgggcctcat ggtcttcaag gacctgcccc acccaaggca gatgcgcgag gtgatcttcc
      361 tgcaacagtc ggccctcacc gccagccagg caccgcccga agaccgccgc aaagccgcca
      421 tgcgcaacct cctacagggg gcctccccta actcccacga ccgccccaca ccccccaacg
      481 ggcaacccca ggtgccgggc aggggcgaga cgcaacttat cgactactcg tccgtttcag
      541 gcattgggct gctgggcagg ttctttccgc tcgcgccgca gaggaacgcg gcgggcgtca
      601 cctggcacaa gcactggatt tacctggtgc gcgggctgct gtggcccgct ctgctgtacg
      661 gcatagcgct cgtggcgtgg ttcgcgatga gggtaacggg ggcttcggcc gcgctcaacc
      721 cggcggagga cgtactggcc tgggtggcgg tgctgctcct gccggtgtgc ctggtgtggg
      781 ccgtctggcg ctgggaggac tggcgcaacg acctctaccg gctggacgct gagcggatct
      841 accacgtcga gagcctgccg ttcggcctgc gcgaagaatc gaccgagacg ctggtcacgc
      901 gcatcaccga cgtgtcgtat tcgctgcctg gcctgctcgc gcacgtgctc aacttcggca
      961 atgtgataat caagacgccg ggcgaatcga gcgagatcgt cttccagggc atcccgcgcc
     1021 cgcgagaggt gcagggggag atcatggccc gcgtagacga gcaccgcctc aaggaccagg
     1081 ccggtatcga cggcgaaatc gaagcctgga tcaaggccta cgacgacgtg aagcggggga
     1141 agtgacgggg gcgagtaccg agtaccgagt gccgagtaaa gagacggaaa cgccaagacg
     1201 caaagaacgc aaaggacgcg caaagtttaa gatggtttgg gtaggtgcta ctcggcactc
     1261 ggtactcgtc actcggtact cgcaacaggg aagggaagca tagcatgcgc gcattccgag
     1321 cggtcctgcc gattttggtg ctcctgggcc tcatgctgcc cggcattgtc cctgtccagg
     1381 cggccccgcc ggctgcttct cccgctcgtg tggcaagcgc cacgttccag actcaggcgg
     1441 gcgtgcggct ggtcaccacg gcggggtttg gggaggacgg tagctacgcg atgggtgcct
     1501 ggttccccgt gcgcgtgatg ctggacaacc cggcgggtgc cggggacatg cgggtgcgcg
     1561 tcgaggtgga cctctcaggc ggttcaggcg gctccggctc ctccagcgta gggacgtacg
     1621 cgcgcgacgt ggacctgccc agcccctccc gcaagcaggt gaccctttac accttcgcgg
     1681 gcaacttcac ccatacggta agggtgcgcc tcctccaggg ggagaaagtc attgcctcgg
     1741 ccaacgtctc ggttgacccc caggagccgg tgaacggcgc tatcgtgggc gtggtctcct
     1801 ccgacgcgtc gctgctcaac gtgtacgaag gcgaggaggt aggcaacccg gagcagccgc
     1861 cttcctcgat gcagttcggc ggccctatgc cccccaccca ggaagcgccc cgcgcccgca
     1921 tcgcccacat acagcccgac gatataccac cactcacggc cggtctcaat agccttgccg
     1981 cgcttgtggt ggacgacgtg gacaccggcg cgctctccca ggagcagcgg gaggccgtgg
     2041 cctcgtgggt gggcaggggc ggcacgctgg tagtcagcgc gcggtcgggc ggggcgaaca
     2101 cgttggcggg tttcgcggac ctcttgcccg tgtccgcgtc gggcacccgc acgctctcca
     2161 gcctcaaagg tatagccgac ctggtgggca ctcctcttga agcctccggc caggtcatcg
     2221 tgcccgacgc gcagttgcgg gccgacctgg tgggcgcacc caggacgctg gcagcgcagg
     2281 acggcgtgcc cctggtggct atacgcgacc tgggcagggg gcaggtggtc tacctggccg
     2341 tgtcgccggg ggccgaccca ctcaagggct gggacgggct ggtgcccctg ctcaagcgcg
     2401 tgctggccga gcaccccgtc acgttagcct acggaggcgc gcgggcgggc ggctacgggc
     2461 catacaacca gttgttcagc acatacggca ctatcttcga cctgcccggt ctcgacctgc
     2521 cggagccgct gctgctggcg gctttcatgc tcatttatat agtgctagtc ggcccggtga
     2581 actacgtcat actcaagcgg atgaggcggg ccgagcttgc ctgggtcact atcccggtga
     2641 tggtggctgt gttcgcggca ctggcatact tcatcgggct gcaatccaag ggtggcgagc
     2701 tactgacgat acggggcaac gtggtgcata ccacgcccgg cctcagcacc gcggaaacgc
     2761 agcagtacct gggcgtcttt tcgcccatac gccgcaccta ccgcctcgaa ataggctccg
     2821 atgccacggc cagcgagatc gacccttatg gctacaacta cagcgggggc ggcagcggcg
     2881 gcaagctccc aaccgtgctg gcaagcggct ccacgaccac cattgagaac gtgaacgtca
     2941 acacgtggag cctgcgcagc ttcatggccg aaagcacggt gcgggtcgag tcgccgttgg
     3001 aggccgattt gcgcctgggc gacaacctga tcgagggcac ggtgcgcaac cgcggcgagg
     3061 tgggcttgga ggacgtggtg ctcgtgcgtg ggcaggccat acagtacata ggcttcatcg
     3121 gccccggcaa ccagaccccg gtcaagctga acatcgcgca cgccccgttc cacaccacct
     3181 ccccggtgcc gctgctgccc ctgccgccgg gggtggaaga ccccagccag tcgggagggt
     3241 actatggtgg gggccaggac agtaaccagc agcgcgacta caaccgccgc gtgcagatgc
     3301 tcagcaccgc gctggagccg ttgctggtca acgagccgcc gtccgacttc acagtgctgg
     3361 ctgtggcctg gggcccctcc ccgccgagtg agtttcgggt gctcgaccgc accacccgca
     3421 ccgaggacct gaacgtgtgg accagccgct tgcaggtggc gggcggcgat ggggggcagg
     3481 gtcgaataac ccagggtacg ctgcccgcaa tgcagtacgt gcccgacgac aacccggcgt
     3541 ggaagccgtt cgacgggtct gaagtgtctc tcaatccgtt caccacggtg cagttccgcc
     3601 tgccccaggg cacgcagccc ggcagcctca ccctggaata tgaagcgcgc accagcctgg
     3661 cgggcaaccc gctggaggta ttggccttca acgtccgcac cggcgcgtgg gacaggctgg
     3721 ccgagatctc aggcggccaa tcgcagcaaa cgagcgtccc gataaacaac ccgctcgatt
     3781 acaccggccc cggcggcgac gtgaccctgc ggattgcgcc caccgccggc tcaacggcca
     3841 ccaggatcgc tttcgacacg ttcaccctgt cgctcaacga cacgcagtag aaagtaagat
     3901 gtcgaattgc agcaccgaaa tccccctctt gtcattccga gcgtagcgag gaatcaggtg
     3961 gcagg
//
LOCUS       DASRWZ010000609         5279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_940098, whole genome shotgun sequence.
ACCESSION   DASRWZ010000609 DASRWZ010000000
VERSION     DASRWZ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940098"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..662
                     /locus_tag="VFH60_09590"
     CDS             3..662
                     /locus_tag="VFH60_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6314076.1"
                     /translation="MKGVSLTVERGEIVTLIGSNGAGKTTTLRTISGILRPRKGNVRM
                     DGKRIDNIAPDQLVKLGIAHSPEGRRIFSRMTVQENLEMGAFARNDRAGIAQDMERVL
                     TLFPRLRERLVQRGGTLSGGEQQMLAIGRALMSRPQVLLLDEPSMGLSPILVETIFNI
                     IRDLNESGSTILLVEQNALLALQVAHRGYVIETGQIVLEDTGANLLQSEQVRKSYLGE
                     D"
     gene            808..1890
                     /locus_tag="VFH60_09595"
     CDS             808..1890
                     /locus_tag="VFH60_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET6314077.1"
                     /translation="MSSLPTKGPSARQTKLISQAIDLAIEEVGPTLPAWRLEHLALDG
                     GDDETGDWSARKEDANARAAARDPGVIAYIGPYASGAAAVSLPILNRGGLLQGLPAAT
                     WPGLTQAGWAAGEPERYYPTGVQTMVRLMPPDSAQALVAANKAHRLGAATAFIVHDGS
                     DYSLGMAAAFQGAAEKLGIRVLGRANAGIDSEEWWSSVEEADVVFLAPSNLSVAAAAA
                     RGMAAQPPRMAVFSTDVTLSDQLSQEDRQLIEGWYVVFNGDATPGEPGRFEDFDRRFQ
                     ARYGEQPSQYAANAYDLTAAVLEATRRAGPDRGKIATEVLSGTYEAAILGPLRFAPNG
                     DVQGGQLTLYRLSGGEFVVQEELAVP"
     gene            complement(1915..2139)
                     /locus_tag="VFH60_09600"
     CDS             complement(1915..2139)
                     /locus_tag="VFH60_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314078.1"
                     /translation="MANEQEGDDSYRQLVELEELESLLEEVEEAGLDSGADWGALPEE
                     LREQLASLGVGNMSELVARISFMHAELDEQ"
     gene            complement(2163..2984)
                     /locus_tag="VFH60_09605"
     CDS             complement(2163..2984)
                     /locus_tag="VFH60_09605"
                     /inference="COORDINATES: protein motif:HMM:NF040134.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314079.1"
                     /translation="MASLLTVGLGILAALQRGEVATEASAQVNWALGAALVAVAAGGS
                     VWLRLGQGQGAKTVLAPDYLPLLRVPVHALLPALLVVGFAIFVRLFSGSWFEVVVIGL
                     AWFSFVTVYWALAHAADTADRYFGLAQTALNICAHLTAFLLYSTIYGLKVRALYSATA
                     VGLVTALLVYEMLRRDATWHLALGRPVEGRRSTQVVLSAAAGLVLGQLTWGLNYWAAL
                     TTLIGGACLLLALYTTYGIVSAYVDQRLNRGTVLEYGGVGTLGLLAVFASAFFGQ"
     gene            complement(3012..3644)
                     /locus_tag="VFH60_09610"
     CDS             complement(3012..3644)
                     /locus_tag="VFH60_09610"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET6314080.1"
                     /translation="MPIVKATTEAWQQAREVIEGGGLVAFPTDTVYGLGCDPYNVRAI
                     ERLYEAKGRDSSKALPLLLSGVHRITEVATGLPATATVLAERFWPGALTLVVGRQPSL
                     PEELGGGESIAVRVPRHAQLRDFLEMCGGALAVSSANLSGQPDALDAAQAAAYLEDRV
                     DLIVDGGRSPGGVPSSVVDCTLPVPRLLREGAITEEDIRAALAAWEEQRA"
     gene            complement(3656..4783)
                     /locus_tag="VFH60_09615"
     CDS             complement(3656..4783)
                     /locus_tag="VFH60_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET6314081.1"
                     /translation="MKGIVAGPVADAGVLTEKDRFEAALRLRRDRVYEYLDAWPGSDK
                     FQPGDIHDALFSYVRRRGKALRPLLLLLSCGAVGGHEDQALPAAAAVEVFHTWTLVHD
                     DIIDRDDTRRGHPTVHAMYRDHAVLAHGHDEPDAAHYGTTVAILAGDVQQAWTYGLLS
                     DLAERGVPTALVLELIQRMATDLTPRLLEGEMLDVQYSLMPAEALDEDRILHMLTMKT
                     GTLLEYAAWCGARIGLGAQPDIDGVAERLGRFASLCGTAFQLHDDVLGLTADEATLGK
                     PVGSDIREGKRTLLVYKALDRTGAEERQFLLSVLGKGDATAEEVARAVGILDSSGARD
                     EVAALARGYVDQALSLLESLPAGEYRDLLRSWALFMLAREH"
     gene            complement(4784..>5279)
                     /locus_tag="VFH60_09620"
     CDS             complement(4784..>5279)
                     /locus_tag="VFH60_09620"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET6314082.1"
                     /translation="RRERAAEGRNGSSDGSWEKSGWAVLPDLVVIDGGKGQLNVAYEL
                     LTSVGLRDVPLISLAKREEEVFLPGRSEPVVLDVRSEALKLLQRIRDEAHRFSNSYNK
                     KLGSKRGTKSKLDEVPLIGPVRKKALYQKFGSLKGMREASIEELMSVKGMDRAAAQSI
                     KEHL"
BASE COUNT          984 a         1805 c         1595 g          895 t
ORIGIN      
        1 cgctgaaagg tgtctcgcta accgtggagc ggggcgagat agtcacgctc atcggcagca
       61 atggcgcggg caagaccacg actctgcgca cgatttccgg catcctgcgg ccgcgcaagg
      121 gcaacgtgcg catggacggc aagcgcatcg acaacatcgc cccggaccag ctcgtgaagt
      181 tgggtatagc ccactcaccg gaagggaggc gcatcttcag ccgcatgacc gtgcaggaga
      241 acctggagat gggggccttt gcccgcaacg accgggccgg tatcgcgcag gacatggaac
      301 gggtgctgac tctcttcccc cgcctcaggg aacgcctggt gcaacggggc ggcactctgt
      361 cgggtggaga gcagcagatg cttgcgattg gccgtgccct gatgtcacgc ccgcaggtgc
      421 tcctcctgga cgagccgtcg atgggcctct cccccatcct agtcgaaacc atcttcaaca
      481 ttattcgcga cctgaatgag agcggttcta ccattctcct cgtcgagcaa aacgcgctcc
      541 tggcgttgca ggtagctcat aggggatatg taatcgaaac gggccagatc gtgctggaag
      601 atacaggcgc gaacctcctg cagagcgagc aggtgaggaa gtcctacctg ggtgaggact
      661 aggcaccttc actgcctgag gagtatgtcg ctatccaggc tattgacctc atgtgcggct
      721 gccatactgg cagtcgtttt gctttccgcc tgtggaggcg atggtgcaac tgaactgccg
      781 cctgctggcg gaaacacact ccgcatcgtg tcctctctgc ccacaaaggg tccgagcgcc
      841 cgccagacaa agctgattag ccaggccatc gacctcgcga ttgaggaggt cggccccacg
      901 ctacccgcgt ggcggttgga acacctggca ctggacggtg gcgacgacga gacaggcgac
      961 tggtcggccc gaaaagaaga cgcgaatgct cgtgctgcag cgcgcgaccc cggcgtgata
     1021 gcctacatcg gcccttacgc ttccggtgcg gcggcagtct ccctgcccat actgaaccgg
     1081 ggaggtctgc tacaggggct accggcagcc acctggccgg gcctgacgca agcggggtgg
     1141 gcggcgggcg aaccggaacg ctactatccc accggcgtgc agacgatggt gcgcctcatg
     1201 ccccccgact ctgcacaggc gctcgtcgcc gctaacaagg cacaccgatt aggagcggcc
     1261 acagccttca tcgtccacga cggaagcgac tacagccttg gtatggcggc ggcgttccag
     1321 ggcgcggctg aaaagctggg aatccgcgtg ttaggccgcg ccaatgccgg tatcgactcg
     1381 gaagagtggt ggagcagtgt tgaggaagcc gacgtggtgt ttctcgctcc cagcaacctc
     1441 tctgttgcgg ctgcggctgc caggggcatg gccgcccagc ctcccaggat ggcagtcttc
     1501 agcaccgatg tcaccctgag cgaccaattg tcgcaggagg accggcagct aatcgaaggc
     1561 tggtatgtgg tcttcaacgg agacgctacc ccaggcgagc cagggcgctt cgaggacttc
     1621 gaccggcgct tccaggcgcg ctacggagag cagccctccc aatacgccgc caacgcctac
     1681 gacctgactg ctgccgtgtt ggaagccaca aggcgcgcag gcccagacag gggcaagata
     1741 gccacagagg tgctttcggg aacctacgag gccgcaatcc tggggccgct gcggttcgcc
     1801 cccaatggcg atgtgcaagg agggcaactg acactctacc ggctgtccgg cggggagttc
     1861 gtggtgcaag aggagcttgc agtgccgtga ggcgtcggct gtggtgtcgg tccgctattg
     1921 ctcgtcaagc tctgcgtgca tgaaggagat acgtgccaca agttcgctca tgttgccgac
     1981 gcccagggag gcaagttgct ctcttagctc ttcgggtagc gcgccccaat cggctcctga
     2041 atcgagcccg gcctcctcca cctcctccag caggctttca agctcctcaa gctcaactaa
     2101 ctgccggtag ctgtcatcgc cttcctgctc gttagccaca gtggtacccc cttctccccg
     2161 gcctactggc cgaaaaacgc cgacgcaaat acggcaagca gccccagcgt gccgacgccc
     2221 ccgtactcca ggaccgtgcc ccggttgaga cgctggtcca cataagccga aacgatgccg
     2281 taagttgtgt agagggcgag caagaggcac gcgccgccga tgagcgtggt gagggcggcc
     2341 cagtagttga ggccccaggt cagctgcccc agcacgagac cagccgcggc agaaaggacc
     2401 acctgggtgc tcctgcggcc ctccactggc cttcccagtg ccaggtgcca cgtggcatcg
     2461 cgccgcaaca tctcgtacac cagcagggcg gtcacgaggc ccacggccgt cgcggagtag
     2521 agcgcccgga ccttcagtcc gtaaatagtg ctgtacaaca ggaaagcggt caggtgggcg
     2581 cagatattca gtgccgtttg ggcgaggccg aagtagcggt cggctgtgtc ggcggcgtgc
     2641 gccagcgccc agtacacggt gacgaatgag aaccaggcca accctattac cacaacctcg
     2701 aaccagctac cggagaaaag cctcacgaag atggcaaagc ccacaaccag tagcgccgga
     2761 agcagggcgt ggaccggcac cctcagcagc ggcaagtagt ccggcgcaag gaccgtcttt
     2821 gcaccctgac cttgccccag tcgcagccac accgacccac cggccgcgac agccaccagg
     2881 gcagcgccca aagcccaatt cacctgcgcc gacgcctcgg tcgcgacctc accacgctga
     2941 agggccgcca gtatgccgag gccgacagtt agcaaactag ccaatatgaa aacacgctca
     3001 gtacgcatgc ctcaggcccg ctgctcctcc cacgccgcca gcgccgcgcg aatgtcttcc
     3061 tccgtaatcg caccttcccg caagagcctg ggcacgggca acgtacagtc taccacgctg
     3121 gaaggcacgc cgccggggct gcgcccacca tccactatca ggtcaactcg atcctcaagg
     3181 tacgcagccg cctgcgctgc gtcaagcgca tccggctggc cggacaggtt cgcgctgctg
     3241 actgccaggg caccgccgca catctcgagg aagtcccgca attgcgcgtg cctggggaca
     3301 cgcaccgcaa tggactcgcc gcctccaagc tcctccggaa gcgacggctg ccgccctacc
     3361 accagcgtaa gcgcacccgg ccaaaatcgc tctgcaagca ccgtggccgt tgccggtagc
     3421 ccagtggcca cttccgtaat ccgatggacg cccgacagca ggagcggcag cgccttgctc
     3481 gaatcgcggc ccttcgcctc gtaaaggcgc tcgattgccc gtacgttata cgggtcgcag
     3541 ccgaggccat agaccgtgtc ggtggggaac gccaccaacc cgccgccctc gatgacctct
     3601 ctggcctgct gccatgcttc ggttgtagcc ttgacaatgg gcatcgcgta attacctagt
     3661 gctcgcgcgc cagcatgaat agcgcccagg aacgcagcaa gtcccggtac tctcccgcag
     3721 gcagcgattc tagcaacgaa agcgcctggt caacgtaccc gcgcgccaga gcggccacct
     3781 cgtcgcgagc cccgctcgag tccaggatgc cgaccgcgcg cgccacctct tcggcggtag
     3841 cgtcaccttt cccgagcacc gaaagcagaa actgcctctc ctccgcgccg gtcctatcga
     3901 gcgccttgta gaccagcagg gtgcgcttgc cctctcgtat gtccgagcct acgggcttgc
     3961 ccagtgtggc ctcgtccgcg gtcaggccaa ggacgtcatc gtgcagctga aaagccgtgc
     4021 cgcacagcga agcgaacctg ccaagccgct ccgctacccc gtcaatatcg ggctgtgcgc
     4081 ccagtcctat cctcgccccg caccaggcag cgtattcgag cagcgtgccg gtcttcatcg
     4141 tcagcatgtg gagaatgcgg tcctcgtcca acgcctcggc gggcataaga gagtactgga
     4201 catccagcat ctcaccttcc agcagccggg gcgtcaggtc tgtcgccatc ctctggatga
     4261 gttccagcac cagcgccgtg ggcactcccc gttccgcgag gtccgacagc aggccatagg
     4321 tccaggcctg ttgcacgtcg cccgccagga tggcaaccgt tgtgccgtag tgggcggcgt
     4381 ccggctcgtc gtgcccgtgc gccagcactg catggtcgcg gtacatggcg tgcacggtgg
     4441 ggtggccgcg ccgcgtgtcg tcccggtcga taatgtcgtc gtgcaccagc gtccaggtgt
     4501 ggaacacctc tacggctgcg gcggccggta gcgcctggtc ctcgtggcct ccaaccgcgc
     4561 cgcaggacag cagcagcagg agcggccgca atgctttccc gcgccgccgg acgtaactga
     4621 agagcgcgtc gtgtatgtcc ccaggctgga acttgtcgga gccgggccac gcatccaggt
     4681 actcgtacac acggtcccgc cgcaggcgca aagctgcctc aaaacggtct ttttcggtca
     4741 gcaccccggc atccgccacc ggaccggcta caattccctt catctacagg tgctccttga
     4801 tgctctgcgc ggccgcccgg tccatcccct tgaccgacat cagctcctcg attgaggcct
     4861 ctctcatgcc cttgagcgag ccgaacttct ggtacagcgc cttcttccgt acggggccga
     4921 tgagcggcac ttcgtccagc ttgctcttcg tgccgcgctt ggagccgagt ttcttgttat
     4981 agctgttgga gaagcggtgc gcctcgtcgc ggattcgttg cagcagcttc agcgcctccg
     5041 agcgcacgtc gagcaccacc ggctccgacc tacccggcag gaatacctct tcctccctct
     5101 tagccaggga aatcagcggc acgtcgcgca gccctacact ggtaagcagt tcgtaggcca
     5161 cgttcagttg ccctttgccg ccgtctatga ccaccaggtc gggaagcacg gcccaaccgc
     5221 ttttctccca actaccgtcg gagctaccgt tgcggccctc cgccgcccgc tcacgcctc
//
LOCUS       DASRWZ010000610         1735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_323362, whole genome shotgun sequence.
ACCESSION   DASRWZ010000610 DASRWZ010000000
VERSION     DASRWZ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323362"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VFH60_09625"
     CDS             <1..773
                     /locus_tag="VFH60_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6314083.1"
                     /translation="AIGGLVLAVSVLLLGRWRALTMRELGWGVLIGTALWAGYAFQTL
                     GLQGTTASKAGFITGLSVVLVPMLGLFVLRHRPGLWAWAGIALATSGLALLSLRFDER
                     LEVNRGDLLVLGCAFAFAIHIVLVARTAQWADPLRLTTVQVIVAGLLNAVSAALSGQQ
                     VASMSQEVWASVVFLGVAATALAFLVQVSVQRFTTATHTALIFTLEPVFAAIFGSWLD
                     NDVLSISGWVGAGLILGGMLVTELLPYLIKRNAAILGD"
     gene            846..>1735
                     /locus_tag="VFH60_09630"
     CDS             846..>1735
                     /locus_tag="VFH60_09630"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET6314084.1"
                     /translation="MQLTATPPLEVVHFDSIANRRAARYHMTDSLKLKTRADAVRFIN
                     EAGVALLFPGDNIPLPDLWSAINGRERKVPKHHHDWALGKTWDWKDQIPSRKEAWYGK
                     LVRGKPAFVALRDLPAVYALSNNYGELDDYLEAYQDGLLSQEAKAVYEVLLAEGPLPT
                     STLRKSSGLGGGGDNARRFERAIVELQTGLKIVKAGISDSNRWKYCYVYDLLLRWAPN
                     LAEESRNYNSRSAMRHLVARYLQSNLAAPPLLFPRLFAWDANVTLRVIDEMVQDGTLH
                     QVRVLKAPGLTSRAKPTPEG"
BASE COUNT          304 a          557 c          569 g          305 t
ORIGIN      
        1 tcgcaattgg cgggctggtg ctggcggtgt cggtgctgct gctaggcagg tggcgcgccc
       61 tgacgatgcg agagcttggc tggggcgtgc tgattggcac cgcgctctgg gccgggtacg
      121 ccttccagac gctcggcctc cagggtacga cggccagtaa ggcggggttc atcacggggc
      181 tttcggtggt gctcgtgccg atgctcggac tgttcgtgct gagacaccgc cccggactgt
      241 gggcctgggc gggcatcgcg ctggccacgt cggggctggc cctgctcagc ctgcgcttcg
      301 acgagcgact ggaagtcaac cggggcgacc tgctggtgct cggttgcgcc tttgcgttcg
      361 ccatccacat cgtcctggtg gcacgcacgg cacagtgggc cgacccgctg cgactgacga
      421 ccgtgcaggt gatcgtcgcg gggctgttga acgccgtaag tgccgcgctg tccgggcagc
      481 aggtcgcctc catgagccag gaggtgtggg ccagcgtcgt cttcctgggg gtggcggcca
      541 cggcgctggc cttcctcgtg caggtgagcg tgcagcgttt caccaccgcc acccataccg
      601 ccctcatctt cacgctggag ccggtgttcg ccgccatttt cggctcatgg ctggacaacg
      661 acgtgctcag cataagcggc tgggtgggtg ccgggcttat cctgggggga atgctcgtga
      721 cggagctgct accctacctg ataaagagga acgctgcgat tttgggggat tgatttcgca
      781 gttgaggcgc attgccgcta taattgggta gccaatctcc tctagtagaa agagtgtttc
      841 caaacatgca actgaccgca accccgcccc tcgaagtggt gcatttcgac agcatcgcca
      901 accgccgcgc agcccgctac cacatgaccg acagcctgaa gctgaagacg cgcgccgatg
      961 cggtgcgctt catcaacgag gcaggagtgg ccctcctctt tccgggcgac aacattcccc
     1021 tgcccgacct gtggagcgcg ataaacggca gagagcgcaa ggtccccaag caccaccacg
     1081 actgggcgct gggcaagacc tgggattgga aggaccagat acccagccgc aaggaggcct
     1141 ggtatggtaa gctggtacgt ggcaagcccg ctttcgtggc cctgcgcgac ctcccggccg
     1201 tgtacgctct ctccaacaac tacggggaac ttgacgacta cctggaagcc taccaggatg
     1261 gcctgctgag ccaggaagcc aaggcggtct acgaggtgct gctggcggag ggtccgctgc
     1321 ccacgagcac gttgaggaag tcgagcggcc tgggcggtgg gggcgacaat gctcgccgct
     1381 tcgagagggc catcgtagag ttgcagaccg gcctcaagat cgtgaaagcc gggatttccg
     1441 actcgaaccg ctggaagtac tgctacgtct acgacctgct gctacgctgg gcacccaacc
     1501 tggccgagga gtcgcgcaac tacaacagcc gcagcgccat gcgacacctg gtcgcccgct
     1561 acctgcaaag caacctggcc gccccgccgc tgctcttccc ccgcctcttc gcctgggacg
     1621 cgaacgtcac ccttcgcgtc atagacgaga tggtgcaaga cggcaccctc caccaggtac
     1681 gagtgctaaa agcccccggc ctcacaagcc gagccaagcc cacgccggag gggga
//
LOCUS       DASRWZ010000611         5118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1077326, whole genome shotgun sequence.
ACCESSION   DASRWZ010000611 DASRWZ010000000
VERSION     DASRWZ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077326"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VFH60_09635"
     CDS             complement(<1..131)
                     /locus_tag="VFH60_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HET6314085.1"
                     /translation="MSDQFIAEIRIFPFNFAPTGWAFCNGQLMPISQNTALFALLGTT
                     "
     gene            complement(230..721)
                     /locus_tag="VFH60_09640"
     CDS             complement(230..721)
                     /locus_tag="VFH60_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HET6314086.1"
                     /translation="MFAGNFAPNGWLFCEGQTLPISENEVLFQLIGTTYGGDGEETFN
                     LPNLASRVPIHMGTGPDGTTYQIGEAAGTEQETLTTQQIPNHTHPLTASTAPGTQTNP
                     SGNMLAQTAISINPYIEQDPTVAMNAGAISPAGGSQPHENTQPFLCINFIISLFGIFP
                     SQT"
     gene            complement(864..1379)
                     /locus_tag="VFH60_09645"
     CDS             complement(864..1379)
                     /locus_tag="VFH60_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009178584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HET6314087.1"
                     /translation="MPMIGEIRLFAGNFAPNGWAFCKGAPLPISENETLFQLIGTTYG
                     GDGQQSFNLPNLSSRVPIHAGTGPDGTTYQLGEAAGTEQETLTVQQIPNHTHPLVASE
                     APGTQSNPSGNVLAQTAISINPYIEQDPTVAMNAASIAPAGGSQPHENTQPFLCINFI
                     ISLFGEFPRVN"
     gene            complement(1394..1891)
                     /locus_tag="VFH60_09650"
     CDS             complement(1394..1891)
                     /locus_tag="VFH60_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HET6314088.1"
                     /translation="MSEPFLSEIKIVSFNFPPKGWALCNGQFLPINQNQALFALLGTT
                     YGGNGQTTFALPNLRGRVPIHFGNDHDLGEAAGSTSVTVNIQQLPTHLHALNVTNQNG
                     ALANPTNNFLGSVVAMYGAPNNLTTMNSQSVTSVGGSQPHNNMMPYLVLNFIIALQGI
                     FPSRN"
     gene            complement(1997..>5118)
                     /locus_tag="VFH60_09655"
     CDS             complement(1997..>5118)
                     /locus_tag="VFH60_09655"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cadherin domain-containing protein"
                     /protein_id="HET6314089.1"
                     /translation="SEQFTITVTDVNEAPTDIALSPSSVAENQAAGATVGTLSTTDPD
                     AGGSHGYTLVAGAGDTDNASFTISGSALQTAASFDFEGQSSYSVRVRSTDNGGLTTEK
                     QLTISVTNVNETPTDISLSSDNVDEDKPAGSTVGTLSTTDPDAGDTHTYTLVAGTGSA
                     DNASFTVVGDELRTAAAFNFEVKASYSLRVRSTDAGGLFTEKAFTVTVNDVNEAPTNI
                     SLSNSSVEEGSPVASAVGDFSTADADTGDTHTYTLVAGAGDADNASFQIASNHLQTAA
                     VFNYATKSSYTIRVRSTDAGGLFFEKQFTITITRLNQEPVNSVPGPQSVNEDADLVFS
                     TANGNAISISDPDAPSNDVKVSLEVLTGTLTYPTTAGLTFVDGTSNGQSSVNVTGSIA
                     NINAALNGLKYKGNLNFNSTRGDEALTITTNDQDSGGPLFDSDSVSITVNPVNDKPSA
                     TGKTFQAHTNMQISLGGLVAGATDPADEDDAGYSPSFSLVDVVVDNCTGCTVSNVNTA
                     AGTFDFNPAPGGTGSYTLKYRVADSGNPGPGATSDYATITVTVSGPVIWFVNPAAATN
                     GNGTLTSPFNNIASADAVDADNHRIFVHTGTVTGGIALNTGEWLVGQGVSGASFDSVF
                     GITPPAGTAARPAVGGTRPTLQGQVGMDSSSAVRGINVNTATGVAISANTVSNLTIGE
                     AAVSSGNASSVAFVNVTGSVSISSSSFTAGSGDNISVINSSGTLDRITFTSVTVGANG
                     VMGNDGLVFRTSGTAIVKATVQNSTFTAARSHGVEVAASGSSVVDFVFTGNAVSNNNP
                     SIAAGAGNVVIGTGGSALSANVTYNISNNTLRDALGAAITVGKGATGAGDVSGTIANN
                     TIGLASVPNSGSAQGSGIVVGIQGGGSHSATITNNTIVQFTNFGILVQGGNTVAGGGQ
                     GRMTAVIKSNTVSQPSANSVSGGFPTSGLRITAGTNTGDNTKFCLTLGGTAADRNTLT
                     GTGSNLAQDIRLVQARLVSLAVTGYAGAANDNTAMQAYLLSQNIAATAGAANNVSAGG
                     PGYSGTCAP"
BASE COUNT          805 a         1477 c         1735 g         1101 t
ORIGIN      
        1 gtggtgccca ggagcgcgaa gagcgccgtg ttctggctta tcggcatcaa ctggccgttg
       61 cagaacgccc agccggtggg tgcgaagtta aagggaaaga tacgaatttc cgcaatgaat
      121 tggtctgaca tgttcgcctc ctaatagcgc atttcattat cagtgtaggg actccccatc
      181 gggcgttccc ggctgcgtga cacaaacgca gcatgtcttc ggccggtgtc tacgtctggc
      241 tggggaatat cccgaacaga gagatgatga agttgatgca caggaacggc tgcgtgttct
      301 catgcggctg gctgccgccc gcgggcgaga tggcaccggc gttcatcgcg actgtcgggt
      361 cctgctcgat gtacgggttg atgctgatgg ccgtctgggc cagcatgtta ccggaagggt
      421 tagtctgcgt gccgggggcc gtcgaggcgg tcagcgggtg cgtatggttc gggatctgct
      481 gggtagtgag cgtctcctgc tcggtacccg ccgcttcacc gatctggtaa gttgtgccgt
      541 cggggcctgt gcccatgtgg atagggaccc ggctggcaag attgggcagg ttgaacgtct
      601 cctccccatc gcctccgtag gtagtgccta tcaactggaa cagcacttcg ttctcggaga
      661 ttggaagcgt ctgtccctcg cagaaaagcc agccattggg ggcgaaattg cccgcgaaca
      721 ttcggatctc tccgacatat ggttgcatat tgttactcct cagttcttac ctgggcagat
      781 gccctgggtc gcgctgcaaa atagacaggg tatagcctca cagtgtggtg ccggggcgca
      841 ttcgtgtgcg acagcgcctg atgctagttt acccgcggga actctccgaa cagagagatg
      901 atgaagttga tgcacaggaa cggttgcgtg ttctcgtgcg gctggctgcc gcccgcgggc
      961 gcgattgaag ccgcattcat cgcgaccgtc gggtcctgct cgatgtacgg gttgatgctg
     1021 atggccgtct gggccagcac gttaccggaa gggttgctct gcgtgccggg cgcttcagat
     1081 gcgaccagcg ggtgcgtgtg gttcgggatc tgctggacag tgagcgtctc ctgctcggta
     1141 cctgccgcct ctcccaactg gtaagttgtg ccgtcggggc ctgtgcccgc gtggataggg
     1201 acccggctgg agaggttggg caggttgaaa ctctgctgtc catcgccccc gtaggtagtg
     1261 cctatcaact ggaacagcgt ctcgttttca gagataggca gcggtgctcc cttacaaaag
     1321 gcccagccat tgggggcaaa attacccgca aacagcctta tttccccaat cattggcatg
     1381 atgctgatac tccttagttc cggctaggga agatgccttg cagggcaata atgaaattca
     1441 acacaagata aggcatcatg ttattgtgcg gctggctccc gcctacgctc gtcaccgact
     1501 gggagttcat agtcgtcagg ttgttgggcg caccgtacat agcgacgacc gagcccagga
     1561 agttgttggt aggattggcc agggcgccgt tctggttggt gacgttcaac gcatggaggt
     1621 gtgtcggcaa ctgctggatg ttcacggtga cgcttgtgct gccggccgcc tcaccgaggt
     1681 catggtcgtt tccaaaatga ataggcaccc gccctcgcag gttgggcagg gcgaaggtag
     1741 tctgcccgtt gcccccgtag gttgtcccca ggagggcgaa gagggcctgg ttctgattga
     1801 tgggcaggaa ctgcccgttg cagagcgccc agcccttggg cgggaagttg aacgatacga
     1861 tcttgatttc gcttagaaat ggctctgaca tgttacctcc tcaaattggt gcgttgcgtc
     1921 cctgtgaagg gaccgcgaat gtgtaggtgg cttgtagggg aggctgcctg ggcatgaaga
     1981 cagcctcccc aagccgttac ggggcgcagg tgcccgagta gccaggcccg cctgccgata
     2041 cgttgttcgc cgcgcccgcc gtagccgcga tgttctggct caacaggtaa gcctgcatgg
     2101 cggtgttgtc gttagccgcc ccagcgtagc cggttacggc caggctcacc agccgcgcct
     2161 gcaccaggcg gatgtcctgg gccaggttgg agccggtgcc cgtcagcgtg ttcctgtcgg
     2221 ccgctgtgcc gcccagcgtc aggcagaact tggtgttgtc gcccgtgttg gtgcccgccg
     2281 tgatgcgaag cccgctcgtg gggaagcctc cactcaccga gttagccgag ggctgggaaa
     2341 cggtgttgct cttgatcacc gcggtcatgc gcccttgtcc gcctcccgcc acggtgttgc
     2401 caccctggac caggataccg aagttggtga actggacgat cgtgttgttg gtgatggtag
     2461 cgctgtggct gccaccgccc tggatgccca ccacgatgcc cgagccttgc gctgagccgg
     2521 agttcgggac gcttgcgagg ccaatcgtgt tgttggcgat tgtgccgctc acatcacccg
     2581 cgccggtcgc gcccttgcca accgttatcg ccgcgcccag cgcatcgcgt aacgtgttgt
     2641 tcgagatgtt gtaagtgacg ttcgcgctca gcgcgctgcc gcccgtcccg atcactacgt
     2701 tgcccgcgcc ggctgcgatg ctcgggttgt tgttgctcac agcgttgccg gtgaagacga
     2761 agtccactac tgagctaccg ctggcggcga cctccacgcc gtggctgcgg gcagcagtaa
     2821 aggtgctgtt ctgcacggtc gccttcacga tggcggtccc gctggtcctg aacaccaggc
     2881 cgtcgttgcc cattaccccg ttagccccaa ccgtcacact tgtgaaggtg atccggtcga
     2941 gcgtacccga cgagttgatc acagagatgt tgtctcccga cccggcggta aagctgctgc
     3001 tggatatcga caccgagccg gtgacgttga cgaacgccac ggacgatgcg ttgccgctgc
     3061 tcactgccgc ctcacctata gtgaggttgc tcacggtgtt ggccgagatg gctacgcccg
     3121 tggctgtatt gacgttgata ccgcgcaccg ccgagctgga gtccatgcct acctgcccct
     3181 ggagggtggg cctggtgccg ccgaccgcgg ggcgggccgc cgtgcctgcc ggcggggtga
     3241 tgccgaagac cgagtcgaag ctggcaccgc tcacgccctg gccgaccagc cactcgcccg
     3301 tgttgagggc gatgccgccg gtgaccgtgc ccgtgtgcac gaagattcga tggttgtctg
     3361 catctaccgc gtcggcgctc gcgatgttat tgaatgggct ggtcaaggtg ccgttgccgt
     3421 tggtggctgc ggcagggttg acgaaccaga tcacggggcc gctcaccgta acggtgatgg
     3481 tggcgtagtc gctggtcgcg cccggtccgg ggttgccgct gtcggccacg cggtacttga
     3541 gcgtgtagct gccggtgccg cccggtgccg ggttgaagtc gaacgtgccg gccgcggtgt
     3601 tgacgttgga cacggtgcag ccggtgcagt tgtcaacaac cacgtccacc agggagaacg
     3661 aaggagagta accggcatcg tcctcatcgg cagggtcggt tgcgcccgcc accagcccgc
     3721 ccagcgagat ctgcatattg gtgtgggcct ggaaggtctt gcccgtggcc gacggcttgt
     3781 cgttgacggg gttgaccgtg atgctgaccg agtcgctgtc gaagagcggg ccgccgctat
     3841 cctggtcgtt ggtggtgata gtcagggcct cgtcgcccct ggtcgagttg aagttcaggt
     3901 tgcccttgta cttcaggccg ttgagggccg cgttgatatt cgcgatggag ccggttacgt
     3961 ttaccgaaga ctggccgttg ctcgtgccgt ccacgaaggt cagcccggcg gtggtggggt
     4021 aggtcagcgt gccggtcagc acttcgaggc tgaccttcac gtcgttactg ggcgcatccg
     4081 ggtccgatat gctgatcgcg ttgccgttgg cggtggagaa caccaggtcg gcgtcttcgt
     4141 tcaccgactg agggccgggt acgctattga caggctcctg gttgagcctc gtgatggtga
     4201 tggtgaactg cttctcgaag aagaggccgc ccgcgtcggt gctgcgcacg cggatggtgt
     4261 agctcgactt ggtcgcgtag ttgaagaccg ccgccgtctg caggtggttg ctggcgatct
     4321 ggaacgaggc gttgtcggca tcgccggcgc ccgccaccag ggtgtaggtg tgggtgtcac
     4381 ctgtgtccgc gtcggcggtt gagaagtcgc caaccgcgct agcaaccggc gagccctctt
     4441 ccacgctgct gttggatagg ctgatgtttg tcggggcctc gttcacatcg tttacggtca
     4501 cggtgaacgc cttctcggta aagaggccgc ccgcgtcggt gctgcgcacg cgaaggctgt
     4561 agctcgcctt gacctcgaag ttgaaggctg ccgccgtcct gagttcgtcg cccaccaccg
     4621 taaaggaagc gttgtcggcg ctgcccgtgc ccgccaccag ggtgtaggtg tgggtgtcgc
     4681 ccgcgtcggg gtcggtggtg ctcagtgtgc ccaccgtgct gcccgcgggc ttgtcctcgt
     4741 ccacgttgtc gctggacagg ctgatgtcgg taggggtctc gttcacgttg gtgaccgaga
     4801 tggtgagctg cttctcggtg gtgaggccgc cgttgtcggt gctacgcacg cggaccgagt
     4861 agctggactg gccctcgaag tcgaaggagg cggccgtctg taatgcagag ccgctgatcg
     4921 taaaggaggc gttatcggtg tcacccgcgc cggctaccaa cgtgtaaccg tggctgccgc
     4981 ccgcgtcggg gtcggtggtg ctcagtgtgc ccacggtagc gcccgctgcc tggttctcgg
     5041 ccacgctgct gggggagagg gcgatgtcgg tcggggcctc gttcacgtcg gtgacggtga
     5101 tggtgaactg ctcagagt
//
LOCUS       DASRWZ010000612         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_598193, whole genome shotgun sequence.
ACCESSION   DASRWZ010000612 DASRWZ010000000
VERSION     DASRWZ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598193"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1069)
                     /locus_tag="VFH60_09660"
     CDS             complement(167..1069)
                     /locus_tag="VFH60_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET6314090.1"
                     /translation="MVTATTAINTPAPQDRLSPFETLYDTGEGRRMALPAELEGLYGS
                     LRFPAPRGRPHVIGNLVTSLDGVVTLGIPGKAGGKEISGSNTHDRALMGLLRAVADAV
                     IVGAGTLREGKGDALTAANVYPPLAGAYNGLRAGLGKVGEPLSVIVTASGDLDPSMQV
                     FKDGQALVVTTRAGAQQLRQLGLPAEVGVVEAGSQDGRSIPAGAILHAVAQVRACEII
                     LVEGGPHLLGDFFAESVIDEQFLTLSPQVVGRDSSGTRLGLVEGRTFAPDSPVWGRLS
                     VVKRVGSHLFLRYMFDDMTASAAA"
     gene            complement(1088..1753)
                     /gene="folE"
                     /locus_tag="VFH60_09665"
     CDS             complement(1088..1753)
                     /gene="folE"
                     /locus_tag="VFH60_09665"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET6314091.1"
                     /translation="MALKLDIDADIDEEIYTDTGVSIHPRLVSGEQMQKFEGYIAEIF
                     TALGLDLDTPATVDTPRRFMRAMFDATSGYDGDPKLLKVFSTECRGGPDCRLSQVIEG
                     PIPFHALCEHHALPFHGNAYVGYIAHEGIIGISKLTRLVRLYARRFSVQERIGQQVAD
                     TLVSLIQPHGVAVYLEAHHMCMQMRGVREISPMTRTTVWRGAYDGDPAIRSEFFTACG
                     LQR"
     gene            complement(1827..3029)
                     /locus_tag="VFH60_09670"
     CDS             complement(1827..3029)
                     /locus_tag="VFH60_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6314092.1"
                     /translation="MTHHTYLIIGGGMTAHAAIRGIREVDPDGSIALISEESDPPYKR
                     PPLSKKLWAGKRLETVWSKMDDLGVEMHLGRMIVTLDLGNKTVTDTQGIEYTFDKLLL
                     STGGTPRRLPFGGDDIIYYRTLQDYLRLRALTERGERFAVIGGGFIGSEVAAALAMNG
                     KKVAMLLPEAGIGERLYPPDIVEFLTDFYREKGVEVLTGERAAGLEKRGDILTLKTRG
                     GKEIAVDGVVAGIGIEPNVQLAREAGLGVEGERDGNNGIAVDGLLRAGHPDIFAAGDV
                     ALFCDPLLGRIRRVEHEDNANKMGRVAGRNMAGANEPYRHSPFFYSDLFELGYEAVGE
                     LDPQLEIVADWKEPYREGVVYYLRDRRVRGVLLWNVWGQVDAARRLIAEPGPFHAEVL
                     KGRLPEGD"
     gene            complement(3050..>3764)
                     /locus_tag="VFH60_09675"
     CDS             complement(3050..>3764)
                     /locus_tag="VFH60_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314093.1"
                     /translation="LVKPYYGDAAGEQLTVLLKEHIIGAVDLLTAAGNNDTVAVEAAS
                     ERWYANGDDIATFLNSANPQAWPLDVLKAMMKEHLDLTLDEAVAHLKGDYAKDIADYD
                     RVHDHILGMADALSAGIVAQFPEKFDTSAGSIAPGAMPGGEHGPEHVGMPRTGEATGE
                     GYQLIWQLAFAGVLLAGIGWVALRRARRQGGALPAGRVGTWRRSASTLRRPHPTNLAG
                     CQTGWWPQPYYARQTSAKR"
BASE COUNT          648 a         1229 c         1108 g          779 t
ORIGIN      
        1 aagtagtgta tttttccttt gactaaccct gtacccttgt catcctgagc gaagcgaagg
       61 atcatcctcc taagcaatca ctctcccgtg ctgatccttc gcgtcgctca ggatgacaag
      121 gtgtagtgtg gcagtgaatg gatacattac ctgcaagata caccactcag gccgccgcac
      181 ttgccgtcat atcatcgaac atatagcgga ggaagagatg gctaccgacc ctcttcacaa
      241 ccgaaagcct gccccataca gggctatcgg gggcgaaggt ccgtccctct actagcccca
      301 ggcgggtgcc cgaagaatca cggcccacga cctgcgggga gagcgtgaga aactgctcgt
      361 caattacgct ctcagcgaag aaatcgccca gcaggtgagg gccaccctcg accaggatga
      421 tctcgcacgc tcgaacttgc gctaccgcat ggaggatcgc acctgccgga atagatcttc
      481 catcctggct ccccgcttcc accaccccta cttcagccgg taatcctagc tggcgaagtt
      541 gctgtgcgcc tgcgcgggtg gttaccacca gggcctgccc gtccttgaag acctgcatcg
      601 aggggtccag gtcgcccgat gccgtcacga tgacggaaag cggttcgcct actttaccca
      661 ggcccgctct gaggccatta tatgcacctg ccaacggagg gtagacgtta gcggcggtga
      721 gtgcatcgcc tttgccttcg cggagggtgc ccgcccccac tatcacggca tcagccacag
      781 cccgcagcag gcccatgagc gcccggtcgt gcgtattgga gccgctgatc tcttttccac
      841 ccgccttgcc gggtataccc agcgtcacga caccatccag cgaggtgacc aggttgccga
      901 tcacgtgtgg gcggccacgt ggggccggga agcggaggga gccgtagagg ccttccagct
      961 cggcaggtaa cgccatcctt cgcccctcgc cggtgtcgta caacgtctcg aacggcgaga
     1021 gacggtcctg aggtgctggc gtgttgattg ccgttgtcgc tgttaccact atcgcaacca
     1081 tcctctccta tcgctgcaac ccacaggcgg tgaagaactc ggaacggatg gccgggtcgc
     1141 catcatatgc cccacgccag acggtggtgc gggtcatggg ggagatctct cgcacgccgc
     1201 gcatctgcat gcacatgtgg tgcgcctcca ggtagacggc cactccatgc ggctggatca
     1261 gcgagaccag ggtgtcggcg acctgctggc cgatacgctc ctgcaccgag aagcgcctgg
     1321 cgtagaggcg caccagccgc gttagcttgg atattccgat gatcccttcg tgcgctatgt
     1381 acccgacgta ggcgtttccg tggaacggga gggcatggtg ttcgcacagg gcgtggaagg
     1441 gtatcggccc ttcgatcacc tggctcaggc ggcagtcagg accaccccgg cattccgtac
     1501 tgaacacctt gagcagcttc gggtcgccgt cgtagccgct ggtcgcatcg aacatggctc
     1561 gcatgaagcg acgaggcgtg tccacggtgg caggcgtatc gaggtccagc ccaagtgctg
     1621 tgaatatctc cgctatgtag ccctcgaact tctgcatctg ctcacccgat accaggcgtg
     1681 ggtgtatact cacaccggta tccgtatata tctcctcatc tatatctgca tctatatcca
     1741 gcttcagtgc catttgctta tcctttcaca tgctctcgtg tatcggtatt cggcaagttt
     1801 aattcaatgc agcaaacgcc ctggtgctaa tcgccttccg gcagcctccc cttgaggact
     1861 tcggcgtgga acggccccgg ttcggctatg aggcggcggg cggcgtccac ctgcccccac
     1921 acgttccaca ggagcacgcc tcgcacgcgg cggtcgcgca ggtagtagac gaccccctcg
     1981 cggtacggct ccttccagtc ggccacgatc tcaagctggg ggtcaagctc gcccaccgct
     2041 tcgtacccca gttcaaagag atcggagtag aagaaaggcg agtgccggta cggctcattc
     2101 gcgcccgcca tgttcctgcc cgcgactctg cccatcttgt tggcgttgtc ctcgtgctcg
     2161 acgcggcgta tcctgcccag gagcgggtcg caaaagaggg cgacgtcccc cgcggcgaag
     2221 atgtcggggt ggcctgcgcg cagcaacccg tccaccgcta taccgttgtt cccatcacgc
     2281 tcgccctcca cgcccaggcc cgcttctctg gcaagctgca cgttcggctc gataccgatg
     2341 cctgccacca ccccgtccac agcgatttcc ttgccgcccc gggtcttcaa ggtcagtatg
     2401 tcgccacgct tttccaggcc ggccgctcgc tccccggtga ggacctccac ccccttctcc
     2461 ctgtagaaat cggtcaagaa ctctactatg tcaggtgggt ataggcgctc tccgatccct
     2521 gcttcgggaa ggagcatcgc taccttcttg ccgttcatcg ccagggcggc ggcgacctcc
     2581 gagccgatga acccgccgcc gatgacggcg aaccgctccc cacgctcggt aagggcgcgc
     2641 agccgcagat agtcttggag cgtgcggtag tagataatgt cgtccccgcc aaaggggagc
     2701 cgcctgggcg tgccgccggt agagagaagg agcttgtcga aggtgtactc gatgccttgc
     2761 gtgtcggtta cagtcttgtt gcctaggtcc agcgtaacga tcatccgacc caggtgcatc
     2821 tccacgccca agtcgtccat cttgctccag acggtctcta ggcgcttgcc cgcccacagc
     2881 ttcttggaaa gtggtggacg tttgtatggt gggtcgctct cctcgcttat gagagctatc
     2941 gagccgtccg ggtccacctc gcggatgccg cgtatggcgg cgtgagcggt catgccgccc
     3001 cctatgatca ggtatgtatg gtgtgtcatt tttctgcctc tcttaatcgt tatctctttg
     3061 ctgacgtttg ccgtgcgtaa taaggctgcg gccaccagcc cgtttgacat cccgccaggt
     3121 tcgtcgggtg gggcctgcgg agggtcgacg ctgaccttct ccaggttccc acccgaccgg
     3181 ccgggagggc tccaccctgg cggcgggccc gtcgcagagc cacccatccg atgcccgcga
     3241 gcagcacgcc cgcgaacgcg agttgccaga taagttggta tccctcgcct gtcgcctcgc
     3301 cggttcttgg cataccaacg tgctcggggc catgttcccc gccgggcata gccccaggcg
     3361 ctatagaccc ggcagacgtg tcgaacttct cagggaactg ggcgactatg cccgcgctga
     3421 gggcgtcggc catgcccagg atgtggtcgt gtactctgtc gtagtcggct atgtctttgg
     3481 cgtagtcgcc cttcaggtgg gccacagcct cgtccaacgt gaggtccagg tgctctttca
     3541 tcatcgcctt cagcacatcg agcggccagg cttgggggtt ggcgctgttg aggaaggtgg
     3601 ctatgtcatc gccgttcgcg taccaccgct cgcttgccgc ctccactgcg acggtgtcgt
     3661 tattgcctgc ggcggtgagg aggtcgaccg cgcctatgat gtgctccttc agcagcacgg
     3721 tcagttgctc gcccgccgcg tcgccgtagt agggcttgac cagg
//
LOCUS       DASRWZ010000613         5210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_734880, whole genome shotgun sequence.
ACCESSION   DASRWZ010000613 DASRWZ010000000
VERSION     DASRWZ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734880"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFH60_09680"
     CDS             <1..270
                     /locus_tag="VFH60_09680"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET6314094.1"
                     /translation="HMALNITATVLWGLNLLLRWGDLPAATSVGALPLALSVAGTLIL
                     VVSGYLGGRMVYHYGIGIARLSKGRWRRVAERGGARVPNKEEAAG"
     gene            267..1097
                     /locus_tag="VFH60_09685"
     CDS             267..1097
                     /locus_tag="VFH60_09685"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET6314095.1"
                     /translation="MIKHLLQGRPLGHPLHPILNHFPIALFVISLLFDAATLFGYGGN
                     AFVLAAFYTMILGVVTGVLAALTGLADYVDIRADHPGKRNATLHMGLNLTMLVLYAVN
                     LNLRNADLGAGGTPLLPFLLSLAGVGIIGVSGYLGGQLVYDDGVGIGRHLRRADAPPQ
                     TIKVLSSRANADGFVPVADEGRIGDGETLRVQVDRTVMAIVKLEGKTYAFQEFCTHRF
                     GPLSEGNFHDHEVQCPWHRSCFDVRTGKVTQGPAKVDLNVYESKVEGGKILVRMPDSE
                     "
     gene            1334..2311
                     /locus_tag="VFH60_09690"
     CDS             1334..2311
                     /locus_tag="VFH60_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6314096.1"
                     /translation="MVRRSRAGGFALGVVAGVGVGLAAMRVAYAPPDPRLEARARDVQ
                     YMGPWRHGFVMANGVRLHYAEMGSGPLVVLLHGFPQCWYQWRYVMPRLAEQFRVVAVD
                     MRGYNLSDKPEGVRAYSANVIARDIVAFVEALGEERAHIVGHDWGGLIAWHIGANHPE
                     HVNKLVVVNAPHPAAYRREALKLEQLLRSYYVFLFQLPVLPEAMLRLTLRGTLPSSAA
                     VPGAFSPDALDVYQNNISQPGAATAMIHYYRAAFRESLGLMRQDDRTIHIPTMLLWGM
                     KDFALVPRLTEGLEAWVPGIRVERNEESGHWVPEEKPGWVADKLLDFLP"
     gene            2452..3423
                     /locus_tag="VFH60_09695"
     CDS             2452..3423
                     /locus_tag="VFH60_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6314097.1"
                     /translation="MRRHNRWLRPIMVAMLSAFILLNGLAWMQARAMTHYAPGGPRTP
                     RPEALSLGEKAWAVLAGVTVPRPGNNHTPAGVGLEYESRTIPVEGGDYLEGWFVTHAG
                     ANGLVLMFPGYAESKESLLPAASTLHALGYNLLMLDFRGVGGSSGNDTTLGVREAKDV
                     ARAVQYARLQWPGHPLVLYGVSMGGAAVMRAIAVEGVRVNAAIVESPFDSLLHTVRNR
                     FHSMGLPGTPGAELIVLWGSVQQGFNGFEHNPVDYASSIACPTLLLYGDNDPRVTPAE
                     ARSIYDRLPRPKAFASFRGAGHEALAVYAPERWKEQVGQFLAGFTLK"
     gene            3490..4455
                     /locus_tag="VFH60_09700"
     CDS             3490..4455
                     /locus_tag="VFH60_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6314098.1"
                     /translation="MAALIRPAQPETDFVRISQLLTGVEPVPVSVDELVEDESRYVPG
                     KIRRRLVALSEHNYIVGYALAVHYPSQGKGKFHIQIVVDPAFRNRGIGASLYDQALAF
                     VREQGAEVVYCEVQERFPEALRFAQHRGFAIHLHEIGAALDLSTLDERRFHGVLEAVE
                     QSGIRISTVAQEGNTLDNQRRLYEINRIAALDNPSSDGTFPSFEVWLKLIPGSADFQP
                     SGQIVAIDTARADGAYIGFSGVAYIAETNIAETLLTGVAQGYRGRGIATALKLHVIRF
                     AVAHGATQIVTRVNSKNRAMLAINAKLGFRVEPGFHELHLSLTSS"
     gene            4574..5116
                     /locus_tag="VFH60_09705"
     CDS             4574..5116
                     /locus_tag="VFH60_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6314099.1"
                     /translation="MPEELPVEIIRSHNRVKTASARMVDGKLVVRVPAGISLEEEREL
                     VDKLLPKVLKSQRKRQREHLPDLKKRAAELNRVHFGGKLRVNEIKWVGNQEKRYGSCT
                     PSTGTVRISDRIGRMPLWVLDYVIMHELAHLVEANHSPRFWQLVARYPLTERARGDLI
                     AVNLEDEFGPHDLVGETSEE"
BASE COUNT          998 a         1587 c         1641 g          984 t
ORIGIN      
        1 cacatggcgc tgaacatcac ggcgacggtc ctgtggggcc tcaacttgct gctgcggtgg
       61 ggcgacctgc ccgcggctac ctcggtgggt gctctgccgc tcgccctgtc ggtggccggc
      121 acgctcatct tggtcgtgtc gggctacctg ggcggcagga tggtgtacca ctacgggata
      181 ggcatagcgc ggctgtctaa gggcaggtgg cgcagggtgg ccgagcgggg cggggcgcgt
      241 gtgcccaaca aggaggaagc ggcgggatga taaagcactt gctacagggc aggcccctcg
      301 gccaccccct ccaccccatc ctcaaccatt tccccatcgc cctgttcgtc atcagcctgc
      361 tcttcgacgc cgccaccctc ttcggctacg ggggcaacgc ctttgtgctc gccgccttct
      421 acaccatgat tctcggcgtg gtgacgggcg tgctcgccgc ccttaccggc ctggccgact
      481 acgtagatat ccgggcggac caccccggca agcgcaacgc caccctccac atgggcctca
      541 acctcaccat gctggtgctt tacgcggtca acctgaacct gcgcaacgcc gacctcggcg
      601 cgggcggcac gcctctactg cccttcctgc tgtcgctggc cggggtgggc atcatcggcg
      661 tgtccggcta cctgggcgga cagcttgtgt acgacgatgg tgtggggata ggtcgccacc
      721 tgcgccgggc cgatgcgccg cctcagacca taaaggtgct gtcgtcccgc gccaatgctg
      781 acgggttcgt gcccgtggcc gacgaaggca gaataggtga cggggagacc ctgcgcgtgc
      841 aggtggatag gacggtcatg gccatcgtga agctggaggg caagacctac gccttccagg
      901 agttttgcac ccaccgcttc ggtcccctct cggagggcaa ctttcacgac cacgaggtac
      961 agtgcccctg gcaccgctcc tgcttcgacg tacgcacggg caaagtcacc caaggccccg
     1021 ccaaagtgga cctcaacgtc tacgagtcga aggtagaagg cggcaaaatc ctggtgcgta
     1081 tgcccgacag cgaatgattt atgggacaga tttattcacc gcagatgcgc agaggacgcg
     1141 ctgagatcgt gattgtttag tgataggcaa aggcagcatt gtacagtaca agattgccac
     1201 cactcagaac accctcaatt cttctctgcg taatctctgc gctctctgcg cctctgcggt
     1261 gaataaaatc tgtttccctt tgcacggagt agaacccggc aagggtgttg cctattggaa
     1321 ggtggtatcg cagatggtta gaaggtccag ggccgggggg ttcgcgctgg gtgtggtggc
     1381 gggtgtcggg gtgggtcttg cggcgatgcg ggtggcctac gcgccgcccg accccaggct
     1441 cgaagcgcgc gcccgcgacg tgcagtacat ggggccgtgg cggcacggct ttgtcatggc
     1501 gaacggggtg cgcctgcact acgccgagat gggtagcggg ccgctcgtgg tgcttctgca
     1561 cggcttcccg cagtgctggt accagtggcg ctacgtcatg ccccgcctgg ccgagcagtt
     1621 ccgcgtggtc gcagtcgata tgcgaggcta caacctgagc gacaagcccg aaggcgtgcg
     1681 cgcctacagc gccaacgtca tagcccggga catagttgcc ttcgtggagg cccttggcga
     1741 agagcgggcg cacatagtgg ggcacgactg gggcggcctc atcgcctggc acatcggcgc
     1801 gaaccatccg gagcacgtga acaagctggt ggtggtgaac gccccgcacc ccgcggctta
     1861 tcgcagggag gcgctcaagc tcgagcagtt gctgcgcagc tactacgtct tcctgttcca
     1921 actgcccgtg ctgcccgaag cgatgctgcg tctcaccttg cgaggcaccc ttccgtcgag
     1981 cgcggcggta ccgggtgcct tctcccccga cgcgctggac gtgtaccaga acaacatctc
     2041 acagcctggg gccgctaccg ccatgattca ctactacagg gcggcgttcc gcgagagcct
     2101 gggcctgatg cggcaggacg accgcaccat ccacataccg accatgctgc tgtggggcat
     2161 gaaggacttt gccctggtgc cgaggctgac cgagggcctg gaggcgtggg tgcccggcat
     2221 ccgcgtggag cgcaatgagg agagcggaca ttgggtgccg gaggagaagc cggggtgggt
     2281 ggcggacaaa ctgctagatt tcctgccgta aagtggtgtt gtctcattga gaacccctgc
     2341 tgaggtgtag taccggcagg ggtttcgttc gttatatcta tcagtgggta gccgccgcac
     2401 gacgccccgc cctggcatac gatactctcg ttcaaaccac ccctcctgat tatgcgcaga
     2461 cacaaccgct ggctcagacc catcatggtc gctatgctgt ccgccttcat cctcctgaac
     2521 ggcctagctt ggatgcaggc gcgggcgatg acgcactacg cccccggcgg cccgcgcacg
     2581 ccccggccgg aggccttgtc gctgggtgaa aaggcgtggg ctgtgctggc aggggttact
     2641 gtgcccaggc cgggtaacaa ccacactccc gccggcgtgg ggctggaata cgaatcgcga
     2701 accattccag tggaaggcgg cgactacctt gagggctggt ttgtgacgca tgcaggggct
     2761 aatggactgg tgctgatgtt tccggggtac gccgagagca aggagagcct gctccccgct
     2821 gcctcgaccc tgcacgctct gggctacaac ctgctgatgc tcgacttccg cggtgtgggt
     2881 ggctccagcg gcaacgacac caccctgggc gtgcgagagg caaaggacgt agcccgcgcc
     2941 gtccagtacg cccgcctgca atggcctgga caccccctcg tgttgtatgg cgtgtcgatg
     3001 ggcggcgcgg cggtcatgcg ggccatcgcg gtagaggggg tgcgggtcaa tgccgctata
     3061 gtggaaagcc ccttcgacag cttgctgcac accgtccgca accgcttcca cagcatgggc
     3121 ctgcccggta cgcccggagc tgagctgatc gtcttgtggg gcagcgtgca acagggcttc
     3181 aacggcttcg agcacaaccc ggtggactac gcctcttcca tcgcctgccc cacgctcctg
     3241 ctctacggcg acaacgaccc gcgcgtaacc cccgccgagg cccgctctat ttacgaccgc
     3301 ctgcccaggc cgaaggcgtt cgccagcttt cggggggcgg gacacgaggc gctggcggtg
     3361 tatgctcctg agaggtggaa ggagcaggta ggccagttct tggcgggatt taccctcaaa
     3421 tagatattgc gccgaatagc ggcagcaaca ttcggtgaga agaaggaaaa aagagggagg
     3481 gtctacggca tggcagcact gatccgtccg gctcagcctg agaccgattt tgtacgcatt
     3541 tcgcaactcc tgacgggtgt tgagccggtg cctgtctccg ttgacgaact tgtcgaagat
     3601 gaaagccggt acgtccccgg aaagatcagg cgtcggctgg tggcgctaag cgaacacaac
     3661 tacatcgtcg ggtatgcctt agccgtgcat tacccttcac aaggcaaggg taagtttcac
     3721 attcagatag tagtcgatcc ggcctttcgc aatcgcggta tcggagcgtc tctgtatgat
     3781 caagctctcg cctttgttag agagcagggc gccgaggtgg tgtactgcga ggtgcaggag
     3841 cgctttccgg aagctctacg tttcgctcag catagaggct tcgccatcca ccttcatgaa
     3901 ataggcgcag cccttgatct atcgaccctg gacgagagac gctttcatgg ggttcttgag
     3961 gcggtagagc agagtgggat acgaatctcc actgtggcgc aagagggtaa cacgctcgac
     4021 aaccagcgac gcctctacga gatcaaccgg atagcagccc tcgacaatcc gtcgtccgat
     4081 ggcacatttc ccagctttga ggtgtggctc aagctgattc cggggtcggc agactttcag
     4141 ccgtccggcc agatagtagc aatcgatact gctagggccg atggcgcgta cattggcttt
     4201 tcgggcgttg cttatatcgc cgagaccaac attgcggaaa cgctactgac aggagtcgcc
     4261 cagggataca gaggcagggg aatagccaca gctcttaagc tgcacgtcat ccggtttgca
     4321 gtggcacatg gagcaactca gatagtgacc cgcgtcaact cgaagaacag ggctatgctg
     4381 gctatcaatg ccaagcttgg cttccgagtg gagccaggct ttcatgaact gcacctcagc
     4441 ctaacctcgt cataacggtt cttggaggaa tttaccctca aataaatgta agttttaccc
     4501 ggtttggtac gggcattatg tatggcgtat actggtcacg tcaggcacaa gacacccggc
     4561 agccatctcc accatgcccg aagaactacc cgtagagatc atccgctcgc acaaccgcgt
     4621 caagaccgcc tccgcccgca tggtggacgg caagcttgtg gtgcgcgtgc ccgccggtat
     4681 ctcgctggag gaggagcgcg agcttgtcga taagctgctg cccaaggtgc tcaagtcgca
     4741 gcgcaagcgg cagcgcgagc acctgcccga cctcaagaag cgcgcggccg agttgaacag
     4801 agtccacttc ggcggcaagc tgcgcgtcaa cgagatcaag tgggtgggca accaggaaaa
     4861 acggtacggt tcttgcacgc cctcgacggg cacggtccgc atatcggacc gcatcgggcg
     4921 catgccgctg tgggtgctcg actacgtaat catgcacgaa ttggcgcacc tggtggaggc
     4981 caaccactcg ccccgcttct ggcagcttgt ggcccgctac cccctcaccg agcgcgcccg
     5041 cggcgacctg atagccgtca acctggaaga cgaattcggc ccccacgacc tggtgggcga
     5101 gacgagtgaa gagtaacacg taacgcgtaa cacgtaacac caggtacctg tcaggccgtt
     5161 gcagtaatta agaattaaga attaaacatt aagaattcca tgacaaggtg
//
LOCUS       DASRWZ010000614         4200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1008601, whole genome shotgun sequence.
ACCESSION   DASRWZ010000614 DASRWZ010000000
VERSION     DASRWZ010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008601"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..747
                     /locus_tag="VFH60_09710"
     CDS             370..747
                     /locus_tag="VFH60_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HET6314100.1"
                     /translation="MEGWKQRARQLNTEVYALYLAYRDPRVSWPARIFAACVVAYAFS
                     PIDLIPDPIPVLGYLDDLVLIPLGIALARRMIPAYVLDECRERARVEIATKPVNRVAA
                     AVIVAVWLLLAVLAVVWLLNLFS"
     gene            1002..1580
                     /locus_tag="VFH60_09715"
     CDS             1002..1580
                     /locus_tag="VFH60_09715"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HET6314101.1"
                     /translation="MSPLVRVAIMLLLPVIVGALIACDNGSGGTQQGGGTLPANPVAR
                     APYEGVPEGKPKNLSGSVPNPKEVLETAQKGQLPNFLEDVLGPSKQAVTTLYQGAAEH
                     YDEFGYIPCYCGCAIYEHPHHDLAECFIKSMSDDGSVEFTDHSTTCSQCQEAAQMTID
                     GLAKATPLKDIRAAVFNKLNYTGIWTDTPPIQ"
     gene            1667..2947
                     /locus_tag="VFH60_09720"
     CDS             1667..2947
                     /locus_tag="VFH60_09720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6314102.1"
                     /translation="MRLLHIIQRYHPYVGGSELYFQELSERLARAGHSVEVWTTDAWD
                     LDYFWSGRARRVEAVRDRHNGVSIRRFAVRHLPLPAIYYRAWRRGMAILSDLPFPPTS
                     TLYRFSRITPWVPSLRKALKALPPGTFDLVHTTNIPFESMIAAAADYAERVGIPHVIT
                     PFTHLGEPGDRSIRRYYSMRHQLDLERRATRLIVQTRLERDFLGGLGVPTGKMHRVGV
                     GVNPYEVVGGNAERFRKKYDVRGPIVYYLGAAAYDKGTTHTIEAMRHVLARGVNATFV
                     MAGSTMLDKFREYYENLSEEVRERCRWLGFISDEDKRDLLAAGQVFCMPSRTDSFGIV
                     YLEAWLNGAPVIGANAGGVPEVISDGVDGYLVEFGDVPALANMIQFLLQRPDIAREMG
                     KAGRRKVLAEHTWDHKISRIAEIYDEAIREKGAR"
     gene            complement(3187..3999)
                     /locus_tag="VFH60_09725"
     CDS             complement(3187..3999)
                     /locus_tag="VFH60_09725"
                     /inference="COORDINATES: protein motif:HMM:NF044469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(F)"
                     /protein_id="HET6314103.1"
                     /translation="MIEQREMIERVRQICREDPRLVAALMYGSFTRDEGDAYSDIEFV
                     LFFRDEAIGEVEPREWVSQIAPVDMYFVNEFGNGTAIFSNLVRGEFHFDKASDMRLVE
                     GWKDTAWFPSLEATIMVDHTGELASLLRPYITPPRHDTPEQVRALCERFINWVLFGTN
                     VLARGEQARALEILGMAHRYLLWLARLAEGRTEHWPTPSKSLERDISPATYARFKTCT
                     ASLDEGRLWRAYDNAWQWGKELMRVQAEKHGLALPTAVIEAMDARIAGNSNR"
BASE COUNT          778 a         1241 c         1361 g          820 t
ORIGIN      
        1 cgtggcacgg gcaacatgat gggggtgggc attggactcg gcgcgggact gggggccgcg
       61 attggtgcct cgtccggcaa catggcgcaa ggtgtggcga ttggtgtggc cataggtgtg
      121 gcgattggtg ctgcgctcaa cgggcgagtc caatcagggt aggaccgggt cgtagctgtc
      181 ctactcatct ttctaacgag gccaaggacg atgagagcag gggtgctatg gttgctcgat
      241 aggctggtac gcacaggcta tcttgacatc caggtgtgtc aagggacctg ctatgttctc
      301 cccttgcggg caggtataat ggctcatggt cacgttttgc ggtcgagggg cgagttacct
      361 gtgagttggc tggagggctg gaagcagcgc gcccggcagt tgaacacgga ggtgtacgca
      421 ttatacctgg cctaccgcga ccctcgcgtg tcgtggcccg cccgcatctt tgcggcctgc
      481 gtggtggcct atgccttcag ccccatcgac ctcatccccg accccatccc ggtgctcggc
      541 tacctggacg acctggtgct catccccctc ggcatcgcgc tcgcccgcag gatgataccc
      601 gcctacgtgc tggacgaatg ccgcgaacgg gcgcgcgtcg aaattgccac aaagccggtc
      661 aaccgggtgg cggctgcggt gattgttgcc gtatggctgc tgctggcggt gttggcggtg
      721 gtttggctgc tcaacttgtt ctcgtagagg tgtggaggga cagccgcatg gtagccacag
      781 ttggacacct gcaaaacgcc ctgccggtct attggcgggc cgtccctcgt ctatcgtccc
      841 tccctggcat cgcatcccta aatatgggac aatatacctc gcatggctga gcggctgatg
      901 ctgtcgatgt gacggtagat aggagaagca ggttgagtag tgtttccgct ggtaggaaag
      961 taaaaggccg gggtacgcgt tcgcatgagc ctggtagtgg ggtgtccccg ctggtgcggg
     1021 tggcgattat gctgctgctg ccggtgatag tgggtgcctt gatagcttgc gacaacggct
     1081 cgggcggcac gcagcagggc ggtggcacgc tgcccgcgaa tcccgtcgca cgcgcgccgt
     1141 acgagggcgt acctgagggc aagcccaaga acctcagcgg ctcggtaccc aatcccaagg
     1201 aagtgcttga gaccgctcag aagggccaac tacccaactt cttagaggat gtgcttggcc
     1261 cctccaaaca ggccgtgacc accctctacc agggcgcggc ggagcactac gatgagttcg
     1321 gctatatccc ctgctactgc ggctgcgcca tatacgagca cccgcaccac gatctggccg
     1381 agtgcttcat caagtcgatg tcggacgacg gctcggtgga gttcaccgac cacagcacca
     1441 cctgcagcca gtgccaggaa gccgcccaga tgaccataga cggcctcgcc aaagccaccc
     1501 ccctcaagga catccgcgcc gccgtcttca acaagctaaa ttacaccggc atctggaccg
     1561 acacaccccc aatacagtga ttttggattt tagattttgg attttggatt gagacccttg
     1621 cgcgcacctg aatccaaaat ctaaaatcca aaatctaaaa tccaaaatgc gattgcttca
     1681 catcatccag cgctatcacc cttacgtcgg aggctctgag ctttattttc aggagctttc
     1741 cgagcggttg gcgagggcgg ggcattcggt ggaggtgtgg actaccgacg cctgggacct
     1801 ggactatttc tggtcggggc gggcgcggcg cgtagaggcg gtgcgggaca ggcataacgg
     1861 ggtgtctata cggcgtttcg cggtgcgaca cctgccgctg ccggccatct actaccgggc
     1921 gtggcgcagg ggcatggcta tcttgagcga cctgcccttc ccccctacct cgacccttta
     1981 ccggttctcg cgtatcacgc cctgggtacc ttccttgcgc aaggcgttga aggcgctgcc
     2041 ccccggcaca ttcgacctgg tgcacaccac caacatcccg ttcgaatcga tgatagcggc
     2101 ggcggccgat tacgcggagc gcgtgggcat accgcacgtg attacccctt ttacgcacct
     2161 gggggagccg ggcgacagga gcatccgccg ctactacagc atgcggcacc agttggacct
     2221 ggagcgccgg gctacccgcc tcatcgtgca gacccgcctc gaacgggatt tcctgggtgg
     2281 gctgggggtg cccaccggga agatgcaccg ggtgggtgtg ggggtgaacc cctacgaggt
     2341 ggtgggcggc aacgcggagc ggttcaggaa gaagtacgac gtgcgcgggc ctatcgttta
     2401 ctacctgggg gcggcggcct acgacaaggg caccacgcac acgatcgagg cgatgcggca
     2461 cgtgctggcg aggggggtga acgccacctt cgtcatggca ggctcgacca tgctcgacaa
     2521 gttccgcgag tactacgaga acctgtcgga ggaggtgcgc gagcggtgcc gctggctcgg
     2581 cttcatctcg gacgaggaca agcgcgacct gctggcggcg gggcaggtct tttgcatgcc
     2641 ctcgcgcacc gactcgttcg gcatcgtcta cctggaggcc tggctcaacg gcgcgcccgt
     2701 gattggggcc aacgcgggcg gcgtgccgga ggtcatttcg gatggggtgg acggctacct
     2761 tgtggagttc ggggacgtgc ccgccctcgc caacatgatc cagttcctgc tacaacggcc
     2821 tgacatcgcc cgcgagatgg gcaaggcggg ccgccgcaag gtgctggccg agcacacctg
     2881 ggaccacaag atctcccgca tcgcggagat ctacgacgag gcgatacggg agaaaggcgc
     2941 gcgctgaaac atttccgccg ggcgcaggcc gcaagatgta cgtgcaagag gaccaaagct
     3001 cctctctgtc atcctgagcg tagcgaagga tctcaagaag gtgggaagag atgctgtcgc
     3061 accgggagca acggcgcggt gagaattcaa cggcgaggta gaagcagcat cttagatcct
     3121 tcgctacgct caggatgaca gggccactct tgggtgctga tattgggcag gtgcggtcgg
     3181 ggttgttcag cgattggagt tacccgctat tctcgcgtcc atcgcctcga taacggcggt
     3241 ggggagtgct aagccatgct tctccgcttg caccctcatt agctccttgc cccactgcca
     3301 ggcattgtcg tatgctcgcc atagccggcc ctcgtctaaa gaagccgtgc aggtcttgaa
     3361 gcgggcgtaa gtagcggggg aaatatctcg ttcgaggctt ttggaggggg taggccagtg
     3421 ctcggtccgg ccctctgcca ggcgggcgag ccacagcagg tagcggtgcg ccatgccgag
     3481 gatttccagg gcgcgggcct gctccccacg cgccagcacg ttggtgccga acagcaccca
     3541 gttgatgaag cgctcgcaca atgcccgcac ctgttccggc gtgtcatgtc gcgggggcgt
     3601 gatgtagggc cgcaggaggg aggccaactc gcccgtatgg tccaccatga ttgtggcttc
     3661 gagggaaggg aaccaggcgg tgtctttcca gccctccacc aggcgcatgt cggaggcttt
     3721 gtcgaagtgg aactcgccgc gcacgaggtt gctgaagatg gccgtgccgt tgccgaactc
     3781 gttgacgaag tacatatcca cgggcgcgat ctggctcacc cactcacgcg gctcgacctc
     3841 gccaatcgcc tcatcgcgga agaagagcac gaactctatg tccgagtagg catccccttc
     3901 gtccctggtg aacgagccgt acatcagcgc cgcgaccagc cgtgggtctt cccggcaaat
     3961 ttgcctcacc cgctcaatca tctcgcgttg ctcgatcatg acgctcccct cctggtgcat
     4021 tttacaactg ttgcaggatg caagttgtgc atgcaaaggg accaaacgtg cctttgtcat
     4081 cctgagcgta gcgaaggatc aggtgggtag gcgagagtcc tcagcgttgg ggatgctgtc
     4141 ggcattagga atactggtgc atagtaccaa gagtcaagag cgggctactg atccttcgct
//
LOCUS       DASRWZ010000615         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1146437, whole genome shotgun sequence.
ACCESSION   DASRWZ010000615 DASRWZ010000000
VERSION     DASRWZ010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146437"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            432..2213
                     /locus_tag="VFH60_09730"
     CDS             432..2213
                     /locus_tag="VFH60_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET6314104.1"
                     /translation="MKILVTEPLHQAGIDFLREDFEVEVKLGLSPAVLLREIGEYEAV
                     ITRSGTAVTADMLAAGTRDGALKVVGRAGIGVDNIDIDAATRYGVAVVNSPNGNVRAA
                     AEHTIALLFALARNIPQAHDLLHEGVWGKNHFMGCEIAGKTLGIIGLGKVGSHVAKLA
                     KALGMEVVAYDPFVEPRRGIPMLTLDDLLRQADFVTLHVPLTPITTHLIGARELGLMR
                     QDAYLINCARGKVVDEATLYDACKTDAIAGAALDVFEQEPPVGSKLLDLKNVILTPHL
                     GGTTHEAMRHSALEVAAQVKALLHGDYPSHIINPEVLAPAPFDLSALLNRWGRFDRVV
                     FDCDSTLSEIEGINELAAMVGVPYEVAEMTRQAMGGEVPFEEVFARRLDIIQPHRKHL
                     AAVGDLYTSTLVEDASLVAAALAHLHIELRLVSGGYREALLPMADELGVPHEHVHAND
                     LHFDAHGQYTGFDAENPLSRSGGKQDVLSRLPHDAATMFIGDGASDVEVLPHVDMFVG
                     YGGVERRTPVHEISPIYLHGSSLAPLLVMSAGMEGCIKLLSEPRFRPLVIKGFSMLMR
                     EGAADYRPEYRPFFERVRKFCMEGICN"
     gene            2204..3298
                     /locus_tag="VFH60_09735"
     CDS             2204..3298
                     /locus_tag="VFH60_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET6314105.1"
                     /translation="MQLRIPGPTPLPDEVLDAMHRDMISHRGDEFHDLLRDVTAMLKL
                     GFQTENDVLIFPGAGTGGLEASLTNLFSPGDTVISVTVGSFGDRYAEIAERFGLHVVR
                     VPSTWGQAADLDALQHALAATPNLKGVLVTHNETSTGVQNDVEAVSRLVRRAADAQGN
                     SSLLLVDCISSLGAVDIPVDRWDIDVAITASQKAWMAPPGLTMLSVSKRAWAAHSAAK
                     LPRYYWDFTEAKRYLEKWETPYTPAVSLLYALQASLSLITQEGLPNVFARHERLRDRV
                     RSAVRRMGLSLFAPDAIASRTVTAIHVPPGLTASDMLAAMRDRGVIIAGGQDAYKEQI
                     IRIGHMGFVTDQDIHEVMSALEDVAGVLVA"
     gene            complement(3525..4184)
                     /locus_tag="VFH60_09740"
     CDS             complement(3525..4184)
                     /locus_tag="VFH60_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6314106.1"
                     /translation="METGTNLNLTHIVRVSREGSGEKGSPGLLLLHGRGADEADLMGV
                     EAALDPRLTIISARAPFRLGYGYAWYGMAQVGSPEDETLRTSLRELHEFVQGIVPAYR
                     LDPSALFVMGFSQGAVMSAALSLTMPERVRGVVMHSGYVPAESGLDFKVEQAAGKPFF
                     VAHGQYDEVIPVRFGREAHEYLGRIGAEVTYQEYPIGHSISEESLDDLSEWLTNRIDE
                     G"
BASE COUNT          813 a         1577 c         1236 g          821 t
ORIGIN      
        1 aaagggcagt gcaaacggac ttaaagcgga cacttaccaa tgcacgggat aatgtgtata
       61 aaccccttga caccctttct acacttgttt tatcataccc tcatcaggca tagcaatcgg
      121 cgattgtgca tcgaactttc cctcgatgta gccgcgcttc ggacacgtgt aacatcatgt
      181 aggcacgact aagatgcgcc ggaagccccc gttacagggc tagagtgtgt cggcaacctg
      241 ccggagcact cgaagaggct gtgatggcga catcatggcg aaccagggtg gcaccacggg
      301 ctaaggctcg tcctcgagtt gaggacgggc gtttttgttt aagttttgag ttatgagtgc
      361 tgagttttga gttagaagaa caactcaaaa ctcaaaactc aaaactcata actcccaaga
      421 agggggccat cttgaagata ctggtcacgg aaccgctgca ccaggcgggt atagacttct
      481 tacgcgaaga ctttgaagtc gaagtaaagc tggggctgtc gcccgctgtg cttctgcggg
      541 aaataggcga atatgaggcg gtcatcaccc gctccggcac ggccgtcacc gccgatatgc
      601 tggcggcggg cacccgcgac ggcgcgctca aggtggtggg gcgcgcgggc atcggcgttg
      661 acaacataga catcgatgcc gctacccggt atggggtggc cgtggtgaac tcgcccaacg
      721 gcaacgtccg cgccgccgcc gagcacacga tagccctgct gttcgccctc gcccgcaaca
      781 tcccccaggc ccacgacctg ctgcacgaag gcgtttgggg caaaaaccac ttcatgggct
      841 gcgaaatcgc cggcaagacc ctcggcatca tcggcctggg caaagtcggc tcgcacgtcg
      901 ccaagcttgc caaagcgctg ggcatggagg tcgtcgccta cgaccccttt gtcgagcctc
      961 gccgtggcat ccccatgctc accctggacg acctgttgcg ccaggccgac ttcgttaccc
     1021 tccacgtgcc cctcactccc atcaccaccc acctgatagg cgcgcgcgag cttggcctca
     1081 tgcgccagga cgcctacctc atcaactgcg ctcgtggcaa ggtcgtggac gaggccaccc
     1141 tgtacgatgc ctgcaagacc gacgctatcg ccggggccgc gctggacgtt ttcgagcagg
     1201 agccgcccgt cggcagcaag ctcctcgacc tgaagaacgt catcctcacc ccccacctgg
     1261 gcggcaccac ccacgaagct atgcgccact cggccctcga agtggcagcc caggtgaagg
     1321 ccctcctgca tggcgactac ccgagccaca tcatcaaccc cgaagtgctc gcgcccgccc
     1381 cgttcgacct ctccgccctg ctcaaccgct ggggccgctt cgaccgcgtg gtgttcgact
     1441 gcgacagcac cctctccgag atagagggca tcaacgagct tgccgcgatg gtcggcgtgc
     1501 cctacgaggt ggccgagatg acccggcagg cgatgggagg ggaggtgccg ttcgaagaag
     1561 tattcgcccg ccgccttgac ataatccaac cgcaccgcaa gcacctggca gccgtgggcg
     1621 acctctatac ctccaccctg gtcgaggacg cgtccctcgt ggccgccgcc ctcgcccacc
     1681 tgcacatcga gttgcgcctg gtgtcgggcg gctaccgcga ggccttactg ccgatggccg
     1741 acgaactcgg cgtgccccac gagcacgtgc acgccaacga cctccacttc gacgcgcacg
     1801 ggcagtacac cggcttcgac gccgaaaatc ccctgtcccg ttcgggcggc aagcaagacg
     1861 tgctgtcgag gctcccccac gacgccgcca cgatgttcat cggcgacggt gcctcggacg
     1921 tggaggtgct gccgcacgtc gatatgttcg tgggctacgg tggggtagag cggcgcaccc
     1981 ccgtgcacga gatctcgccc atctacctgc acggcagcag cctggccccg ctcctggtca
     2041 tgtcggcggg tatggagggc tgcatcaagc tgctatccga gccccgcttc cgccccctcg
     2101 tcatcaaggg cttctcgatg ctcatgcgcg agggtgccgc cgactaccgg cccgagtacc
     2161 gccccttctt cgagcgagtc cgcaagttct gcatggaggg gatatgcaac tgaggatacc
     2221 cggccccaca cccctcccag acgaggtact cgacgccatg caccgtgaca tgatcagcca
     2281 ccggggcgac gagttccacg acctgctgcg cgacgttacc gccatgctca agctcggctt
     2341 ccagaccgag aacgatgtgc taatcttccc cggcgcgggc acgggcggcc tcgaagcgtc
     2401 cctcaccaac ctcttctcgc cgggcgatac cgtaatctcg gtcacggtag gctcttttgg
     2461 cgaccgctac gccgagatag ccgagcgctt cggcctccac gtcgtgcgcg tgccctccac
     2521 ctggggtcag gccgccgacc tcgacgcgct gcaacacgcc ctggccgcca ctcccaatct
     2581 caagggtgtc ctcgtgaccc acaacgagac ttcgacgggc gtgcagaacg acgtcgaagc
     2641 cgttagccgc ctcgttcgcc gggcagcgga cgcgcagggc aactcctctc tgctgctggt
     2701 agactgcatc tcctccctcg gcgcggtaga catcccggtg gaccgctggg acatcgacgt
     2761 agccattacc gcctcccaaa aagcctggat ggcccctccc ggcctcacca tgctctcggt
     2821 cagcaagcgc gcctgggcag cccacagcgc cgccaaactg ccccgctatt actgggactt
     2881 caccgaggcg aagcgctacc tggagaagtg ggagaccccc tacacccccg ccgtgtcgct
     2941 cctgtacgcc ctgcaagcct ctctctcgct catcacgcag gagggcctcc ccaacgtatt
     3001 cgcccgccac gaacgtctgc gggaccgggt gcgctccgca gtccgccgca tgggcctgtc
     3061 cctcttcgcc cccgacgcga tagcctcccg caccgtcacc gccatccacg tgccccccgg
     3121 cctcaccgcc tccgacatgc tcgccgccat gcgcgacagg ggcgtaatca tcgcgggcgg
     3181 ccaggacgcc tataaggagc aaatcatccg catcggccac atgggcttcg tcaccgacca
     3241 ggacatccac gaggtgatgt cggcgctcga agacgtagcc ggggtgctgg tagcctgaac
     3301 ccgaaaggtg gaaaactaaa cataaaggga ggccggcgca tcaaagccgc cagccttccg
     3361 ttttggacca tccgacacgc tcacttattc ttgtgcacat cggtcgtacc ctggtccttt
     3421 tgcactatct aaccctttct tctctttgcg cgtcctttgc gtcctttgcg tcctttgcgt
     3481 tttcgtctcc tggcgttatt attggcgtcc tggcggtccg tcgtctaccc ttcgtcaatc
     3541 ctgttcgtca gccactcgct gaggtcgtcc aggctctcct cgctgatgga gtggcctatc
     3601 gggtactcct ggtaggtgac ttcggcccca atgcgaccca ggtactcgtg ggcctcgcgc
     3661 ccgaagcgca ccggtatgac ctcgtcgtac tgcccgtggg ccacgaagaa gggcttacct
     3721 gccgcctgtt ccaccttgaa gtccaacccc gactcggccg gcacgtagcc gctgtgcatc
     3781 accacgcccc gcacccgttc aggcatggtt agcgacaggg ccgccgacat gaccgcgccc
     3841 tggctgaagc ccatcacgaa cagcgcggaa gggtccagcc ggtacgccgg tacgatgccc
     3901 tggacgaact cgtgcaactc cctcaggctg gtgcgcaaag tctcgtcctc cgggctgccc
     3961 acctgtgcca tgccgtacca tgcgtagccg taacccagcc tgaaaggggc gcgggcgctg
     4021 ataatcgtca gccgtgggtc gagcgccgcc tccacaccca tcaggtccgc ctcgtccgcc
     4081 cctcgcccgt gcagcagcaa cagtccaggg ctgcccttct ccccactgcc ctcccgcgac
     4141 acccgtacaa tgtgcgtcag gttcagattc gtacccgttt ccatatccac ctctccactc
     4201 caatactgcc ccgcacccac tccacttatt cgcctcattt tactctatcc actctcgtcg
     4261 gatgtattac gatggcctgc cgtgccgtgc cgtgccgtca ccggaagacc ttgtgccgca
     4321 tattgtgtga ccagagtggc ccctgtcatc ctgagcgtag cgaaggatct aagatgttgc
     4381 ctgtaccgct acgttgaaca tcagcaccac aagacgcact attcagcagc attcctgtac
     4441 caccttc
//
LOCUS       DASRWZ010000616         9021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_940766, whole genome shotgun sequence.
ACCESSION   DASRWZ010000616 DASRWZ010000000
VERSION     DASRWZ010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940766"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /gene="gatB"
                     /locus_tag="VFH60_09745"
     CDS             <1..1347
                     /gene="gatB"
                     /locus_tag="VFH60_09745"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET6314107.1"
                     /translation="GMPGTLPAINAEAVRKLLLTGLALNCSIPDFSKFDRKNYPYPDL
                     MKGYQISQYDLPLCVEGYIEVDVPDDRGNAASSFRVGIRRVHLEEDTARLLHRTGPDG
                     PYTLMDVNRAGVPLMEIVSDPDITSPEQAQLFAMKLRHLLRWLGVSTGNMEEGALRVD
                     ANVSVRPVGQAEFGTKVEVKNMNSFKALRAALEYEVARQTGVLRGGGRVVQETRGWVE
                     GRNVTASQRSKEEAHDYRYFPEPDLPPLTFTEADVDEFRAQLPELPDARRERLVRDYG
                     LSPYDAAQLTTSPAIADYYEEAVRGLSSEGARAAAGLVVNDLARLASEAGVAVEESKL
                     APANLRELVELLTGGTINRSIARDLLPELFTGGASPTRLVSERGLGIMRDDSALEIAV
                     DEAIAANPKAVADYLGGKESAIAAFLGPIMRATRGQADANAVRQLLKHKLEALRNA"
     gene            1470..2729
                     /locus_tag="VFH60_09750"
     CDS             1470..2729
                     /locus_tag="VFH60_09750"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET6314108.1"
                     /translation="MADTVNKPNPYLIAVEQFMQAADKLHLDEGMKQILAHPKRELTV
                     HFPVRMDDGSYRVFTGYRVQHSLTRGPAKGGIRYHQDVTLDEVKALAMWMTWKCATVG
                     IPYGGGKGGVVIDPKQHSISELERLTRRFAAEISPIIGPEIDIPAPDVNTNAQTMAWF
                     MDTVSMLRGYPMPGLITGKPIAVEGSLGRNEATARGLQYVVREAVKTRNMSLEGARVV
                     VQGYGNAGAIAARLLSEDGAKIIAVSDSRGGIHNTSGIDPVGALRHKQEHGSLSGFGG
                     SDDVTNEELLEVDCDILVPAALESVITVHNVDRIKTKMIAEAANGPTTPEADRVLYDK
                     GILVLPDILANAGGVTVSYFEWAQNIQGYYWAEDEVNQKLERVMKRSFRDVFETADNN
                     TVDMRTGAYMLAISRVAEVTRLRGIFP"
     gene            3012..3719
                     /locus_tag="VFH60_09755"
     CDS             3012..3719
                     /locus_tag="VFH60_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HET6314109.1"
                     /translation="MDNLSISEAFADLSTPLVADACLRLGLPIRLAPPGLKPIVPGSR
                     LSGRVLPARHYGSVDIFLEAMRAALPGDVLTIDNGGRTEEGCIGDLTALEANAFGLAG
                     MAVWGCHRDTPELLQIGLPIFSYGTCPAGPQRLDARHADALSTARFGSIEVGTGDVLF
                     ADDDGLLFVAHERAEAVLETALGIRRTERQQAEAIRNGTKLSDQLQFEEYLARRATDP
                     AYTFRAHLRSIGGAIEE"
     gene            3766..5709
                     /locus_tag="VFH60_09760"
     CDS             3766..5709
                     /locus_tag="VFH60_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314110.1"
                     /translation="MPDYFDDISTMEELDPEPQPRGRGYAREFVLGALLLLAVIGWAS
                     WLAWQQDVKQQAYQRAEREVAVQGWDSAYRFFVSASGYKDADARAEQIEKLIEERDEH
                     YQAANLATADERWVQALKELQQVRNIQPAYLDVVPMYATVETQVYSDALRGAVALRSA
                     AEPPGLYYRTGSGWVWLEGSDRSSRVLGSTHDSLAYDVPGPDRAPRPTATPLPFNQQP
                     SSGMPELEGRKLKVATLHTTGVRTRDSVLDPARYTIFLPARDGVLAGRYNERPGTYLQ
                     AVRSGYTGWWLDYDYVQDGHPISTTISMGNPSETIMDFARPDQRYVVAIAGGASVRSS
                     KIQLSLGERREDGTYTRRPIYSHVGQLGGAQVSPDGRYMLLTAIAPVDNGIREQQSAL
                     LFDLVAETPPITLTTHTVPIDTIGSFVAFDTRVKGTFLGQGDYAGKLLLVERDDLHLT
                     VSLIDPVDPAARKVLAQIEGNNYLFNVTEKPGRPDLWLSGWSLEGTSSLDFEVSKLLF
                     IRLAPGAEARISYMPYVQTGYARTLGTYGDRLVYEQRTNTNEQASVEVYSVHNRVLDQ
                     TLGVEPTPVYTASTGFILSEGGSAGYSSRNPSWYAGVGLFAYAESGSLYAREYDGGNE
                     VKLESNVWQLIDPSGFFTTTYFR"
     gene            complement(5783..6154)
                     /locus_tag="VFH60_09765"
     CDS             complement(5783..6154)
                     /locus_tag="VFH60_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6314111.1"
                     /translation="MDTPAPYSMNMDTRFAPLELIDVQALADASAEPWYNQTLCRVND
                     CVVRLGVVQGEFHWHRHDEEDEFFYVVEGRFIIDLEGRTVELAPRQGFTVPKGVLHRP
                     RALERTVILMIEGAGVVPTGD"
     gene            complement(6217..6705)
                     /locus_tag="VFH60_09770"
     CDS             complement(6217..6705)
                     /locus_tag="VFH60_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014985146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HET6314112.1"
                     /translation="MDSEEPYTRFIAYLNAGGVPYRLIDHPPEGQTELVSAMRGNTLS
                     QAAKCIVLMVKIGKKVTRYVLAVVPGDRRVDLAAVKALMGGTYVAFASQETAERLAGS
                     VTGTILPFSFNPELELVVDPSLLENDELYFNAARLDRSIALKTADYLVAASPRLGRIA
                     EG"
     gene            6827..7801
                     /locus_tag="VFH60_09775"
     CDS             6827..7801
                     /locus_tag="VFH60_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018535175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6314113.1"
                     /translation="MHTVATVAYDAVNPFELAVATEVFGFERPELGVPWYGFLICGVE
                     PRPIRTSVNMFLSTPYSLQHLAQADTVIVPSSRPGVVPVPEALLAALRQAYERGARII
                     AFCTGAFILAEAGLLTGRRVTTHWAWAAELAERYPGVRVDPMGLYIDDGQVLTSAGTA
                     AAIDLSLHIVRKDYGADIAAAVARRMVVPPHRDGGQAQYIETPLFTTSAQEEPFGTTL
                     SWVACHLHLDLSVEQIAERAMMSPRTFARRFRSTLGITPYRWLIQQRIVLAQRLLETT
                     TEPIEQVAERCGFGSAAALRLHFQRLLHISPQTYRKAFQHEEGKVASG"
     gene            complement(7982..8674)
                     /locus_tag="VFH60_09780"
     CDS             complement(7982..8674)
                     /locus_tag="VFH60_09780"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET6314114.1"
                     /translation="MAEPFLIFGHRGSPKRFPENTIASFDEALRSGADGFETDLRLLS
                     DKTAVLYHDDDFADADVETLSAVEIAERGTVVQQLHDLAAYAERTTMILEVKRSKWED
                     VLLEHIAGWPNVVLASFDHSTIAEVARRNTGIPLGITVYGYLLDLATYASRLGATWCF
                     PNFHYVDEALVASLHARGIKVVPWTANRTRDWERLREIGCDGVITDFPAEAVEWRGQS
                     GQSPVVSRQWSG"
     gene            8739..>9021
                     /locus_tag="VFH60_09785"
     CDS             8739..>9021
                     /locus_tag="VFH60_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6314115.1"
                     /translation="MAKKILLADDSITIQKVVELTFSDGDYEVTAVNNGAKAIAKLAD
                     MRPDIILSDIIMPEKNGYEVCEYVKSHPEYRNIPVVLLTGTFEPFDPDRA"
BASE COUNT         1752 a         2903 c         2799 g         1567 t
ORIGIN      
        1 gggatgcccg gcactctgcc cgccatcaac gccgaggccg ttcgcaagct gctgctcacc
       61 gggttagccc tcaactgctc catacccgac ttctccaagt tcgaccgcaa gaactacccc
      121 taccccgacc tcatgaaggg ctaccagatc tcccagtacg acctgcccct ctgcgtggaa
      181 ggctacatcg aggtggacgt gcccgacgac cgcggcaacg cggcctcctc cttccgcgtg
      241 ggcatacgcc gcgtgcacct ggaggaggac accgcccgcc tgctgcaccg caccgggccg
      301 gacggcccct acaccctcat ggacgtgaac cgcgcgggcg tgcccctcat ggagatcgtg
      361 tcggaccccg acattacctc gcccgaacag gcgcagctct tcgcgatgaa gctgcgccac
      421 ctcctgcgct ggctcggcgt gtccaccggc aacatggagg aaggggcctt gcgggtggac
      481 gccaacgtgt cggtacgccc ggtgggccag gctgagttcg gcaccaaggt cgaagtgaag
      541 aacatgaact cgttcaaggc gctgcgggcg gcgttggagt atgaggtagc gcggcagacg
      601 ggcgtgctgc gcggcggcgg acgggtggtg caggagacgc gcggctgggt cgaggggcgc
      661 aacgtcaccg cgtcgcaacg ctccaaagaa gaggcccacg actaccgcta cttccccgaa
      721 cccgacctgc cgcccctcac cttcaccgaa gcggacgtag acgagtttcg cgcgcagcta
      781 ccggagctac ccgacgcccg ccgcgagcgc ctggtgcgtg attacggcct ctcgccctac
      841 gacgccgccc aactcaccac ctcgcccgca atagcggact actatgaaga ggcggtcagg
      901 gggcttagct cagagggagc cagggccgcc gccggtctcg tggtgaacga cctcgccagg
      961 ctagcgtccg aagcgggcgt agcggtggag gaaagcaagc tcgccccggc taacctgcgc
     1021 gagctggtgg agctactcac cggcggcacc atcaaccgca gcatcgcaag ggacctgctg
     1081 cctgagctat tcaccggcgg cgcgtcccct acccgcctgg tgagcgagcg cggcctcggc
     1141 atcatgcgcg acgactcggc cctcgaaata gcggtagacg aggcaatcgc cgccaacccc
     1201 aaagccgtgg cggactacct gggtggcaag gagagcgcga ttgccgcctt cctcggccct
     1261 atcatgcgag ccaccagggg ccaggctgac gccaacgcgg tccggcagtt gctgaaacac
     1321 aaattggagg cattgcgtaa cgcgtaactc gtaactcgta actcgtaaag tcataccgta
     1381 cgggtataat gagttacgcg ctacgtgtta cgcgttacat gagctatcaa agtcgataat
     1441 tcatctagac tcaatgagga ggctaactaa tggcggacac ggtcaataaa ccgaacccgt
     1501 acctgattgc ggtcgagcag ttcatgcagg ctgccgacaa gttgcacctg gacgagggca
     1561 tgaagcaaat cctcgcccac cccaagcgcg agctgaccgt gcacttcccc gtgcgcatgg
     1621 acgacggcag ctacagggtg ttcaccggct atcgcgtgca gcactctctc acccgcggcc
     1681 ccgcgaaggg tggcatccgc taccaccagg acgtgaccct cgatgaggtg aaggccctgg
     1741 cgatgtggat gacctggaag tgcgccacgg tgggtatccc ctacggtggc ggcaagggcg
     1801 gggtagtcat cgaccccaag cagcacagca tttcggagct tgagcgcctt acccgccgct
     1861 tcgccgccga aatctccccc atcatcggcc ccgaaatcga catccccgcg cccgatgtga
     1921 ataccaacgc tcaaacgatg gcctggttca tggacaccgt gtcgatgctg cgcggctacc
     1981 ccatgcccgg cctcatcacg ggcaagccca tcgcggtcga ggggtcgctg ggccgcaacg
     2041 aggcgacggc gcgcggcctc cagtacgtgg tgcgcgaggc ggtaaagacc aggaacatga
     2101 gcctggaagg ggcgcgcgtg gtggtgcagg gctacggaaa cgccggggcc atcgccgccc
     2161 gcctgctcag cgaggacggg gcgaagatta tagccgtgtc cgacagccgg ggcggcatcc
     2221 acaatacgag cggtatcgac ccggtgggtg ccctgcgcca caagcaggag cacggctcgc
     2281 tcagcggctt cggcggcagc gacgacgtga ccaacgaaga actgctggag gtagattgcg
     2341 acatcctggt gcccgccgcg ctggaaagcg taatcaccgt gcacaacgtg gaccgcatca
     2401 agaccaagat gatagccgaa gccgccaacg gccccaccac ccctgaggcc gaccgcgtgc
     2461 tgtacgacaa gggcatcctg gtgctccccg acatactggc gaacgcgggc ggcgtcaccg
     2521 tgtcctactt cgagtgggcg cagaacatcc agggctacta ctgggccgag gacgaggtga
     2581 accagaagct ggagcgcgtt atgaagcgtt ccttccggga tgtgttcgag accgccgaca
     2641 acaacacggt cgatatgcgc accggggcct acatgctggc catcagccgc gtggccgaag
     2701 tgacccgcct ccgaggcatc ttcccgtaaa ccgcagcggc agagacgaaa tcgcaaagca
     2761 tgccctgagc gcagccgaag ggacgcaaag acgcaaagaa cgcgcagaga ttagggttgt
     2821 tggatgctaa gtaaagagga ccagggtaca tgttgacgaa ccctggtcct ctttgttccc
     2881 actagaccct aacaaccttt gcgcgttctt tgcgtctttg cgtctttgcg ttttcgtctc
     2941 ctataccctg gctgtgattg tacaatggcc ctcaagccgg taaaatggag ggtagtttgt
     3001 tggagggcgc tatggataac ctgagtatta gcgaggcgtt tgccgacctt tcgaccccgt
     3061 tggtggccga tgcctgtttg cggctgggcc tgcccattcg ccttgccccg cccggcctca
     3121 agcccatcgt gccggggagc cgcctgtcgg ggagagtgct gcccgccagg cactacggga
     3181 gcgttgacat cttcctcgaa gcgatgaggg ccgcgctgcc gggcgacgtg ctcaccatcg
     3241 acaacggcgg gcgcaccgag gaaggctgca taggcgacct taccgccctg gaagcgaacg
     3301 ccttcggcct ggcgggcatg gcggtctggg gctgccaccg cgacacgccg gagcttctgc
     3361 aaatcggcct gcccatcttc agctacggta cgtgccctgc cggcccccaa cggctcgatg
     3421 ctcgccacgc tgacgctctc agcacggccc ggttcggcag cattgaagtc ggcaccgggg
     3481 acgtgctgtt cgccgacgat gacggcctct tgttcgtggc gcacgagcgc gcggaagcgg
     3541 tgctcgagac cgccctcggc ataaggcgca ccgagaggca gcaggcggag gccatacgca
     3601 acggcaccaa gctatccgac caactccagt tcgaggagta cctggcccgc cgcgccacgg
     3661 accccgctta cacctttcgg gcgcacctgc gcagcatagg cggggctatc gaggaatagg
     3721 caagccacgc ccggcggttg aagccacatt gcacccaaac ataccatgcc cgactacttc
     3781 gacgacatct ccacaatgga ggaactggac cccgaacccc agcccagggg ccggggctac
     3841 gctcgcgagt ttgtgctggg ggcgctgctg ctgctcgcgg tgattggctg ggctagctgg
     3901 ctggcgtggc agcaggatgt caaacagcag gcttaccagc gggccgagcg ggaagtcgcc
     3961 gtgcaaggct gggactctgc ctatcgtttc tttgtatcgg cctccggcta taaagatgcc
     4021 gacgcgcgcg ccgagcagat agagaagctc atcgaggagc gcgacgagca ctaccaggcc
     4081 gccaacctcg ccacagcgga cgaacgctgg gtccaggctt tgaaagagct acagcaggtg
     4141 cgcaacatcc agcctgccta cctcgatgtg gtccccatgt atgccaccgt tgaaacgcag
     4201 gtatacagcg acgccctccg gggtgcggtc gccctgcggt ccgctgccga gccgcccggc
     4261 ctctactacc ggaccggtag cggctgggtg tggctggaag gcagcgacag gtccagccgg
     4321 gtgcttgggt ccacccacga cagcctcgct tacgacgtgc cgggaccgga tagggcacca
     4381 cgccctacgg ccaccccgct acccttcaac cagcagccgt cctccggcat gcccgaattg
     4441 gagggccgca agctgaaggt cgctaccctc catactacgg gggtcaggac cagggactcc
     4501 gtgctcgacc ctgcccgcta caccattttc ttgcccgctc gcgacggggt cctcgctggc
     4561 cgctacaatg aaaggcccgg cacctacctc caggccgtgc gcagcgggta caccggctgg
     4621 tggctcgatt acgactacgt gcaggacggc caccccattt ctacaaccat atcgatgggt
     4681 aaccctagcg agacaatcat ggacttcgcc cggcccgacc aacgctatgt ggtagccatt
     4741 gccggcggtg ccagcgtcag atccagcaag atacagcttt ccctcggcga gaggagagag
     4801 gatggcacgt acaccaggcg tccgatctac tcgcacgtag ggcagcttgg gggcgcgcag
     4861 gtcagccccg acgggcgata catgctgctc accgccatcg ctccggtgga caacggcatt
     4921 cgcgagcagc aatcggccct gctcttcgat ctcgtggccg agacgccgcc aatcaccctc
     4981 accacgcata cggtgcccat agatacgatc ggcagcttcg tcgcattcga tacgcgggtg
     5041 aaaggcacct tcctgggcca gggtgactac gccgggaagt tgctgctcgt ggaacgcgac
     5101 gacctgcacc tcaccgtgag cctcatcgac ccggtggacc cggccgcacg caaggtgctg
     5161 gcgcagatcg agggcaataa ctacctcttc aacgtaacgg aaaagccggg taggccagac
     5221 ctatggttga gcgggtggag tttggaaggc acctcttcgc tcgatttcga ggtttcgaag
     5281 ctgctcttca tcaggctcgc tcccggcgcg gaggcgagga tatcttacat gccctacgta
     5341 cagacaggct acgcgcgcac cctgggcacc tacggcgacc gcctggtgta cgaacagcgc
     5401 accaacacca atgagcaggc tagcgtcgag gtgtatagtg tgcacaaccg ggtactcgac
     5461 cagacactgg gggtcgagcc tacgccggta tataccgcct ccaccgggtt catcctgtcc
     5521 gaaggtggaa gcgcgggtta ttcgtcgcga aacccctcct ggtacgccgg cgtaggactt
     5581 ttcgcctacg ccgaaagtgg cagcctctac gcgcgcgagt acgacggcgg caacgaggtc
     5641 aagctggaaa gcaatgtgtg gcagttgata gatccgagcg gcttcttcac caccacgtat
     5701 ttccgctgac ccttgagcct gggctgccat acttccacag tagccaccgc cttcaccgga
     5761 cggttcgcag gcaggctgtg gctcaatctc ccgtggggac cacccccgcc ccctctatca
     5821 tgagaatgac ggtgcgctca agcgcgcgtg gccggtgcag cacccccttg ggcaccgtga
     5881 acccctggcg cggggccaac tccacagtgc ggccttccag gtcgatgatg aaacgtccct
     5941 ccaccacgta gaagaactcg tcttcctcgt cgtgcctgtg ccagtggaac tccccctgca
     6001 ctacgccaag ccggaccacg cagtcgttta cccggcagag cgtctggttg taccagggct
     6061 cggcggacgc atccgcaagc gcctgtacat cgataagttc cagaggagcg aaccgtgtgt
     6121 ccatgttcat agagtacggt gcaggtgtgt ccatagtacc ctcctcgcag ggagcattgt
     6181 tgctgccgta tagagccacg cctggagcat aagttatcag ccttcagcaa tccgtcccaa
     6241 ccgagggctg gcggccacca ggtagtcagc cgtcttcagc gcaatggagc ggtccaggcg
     6301 tgcggcgttg aagtacaact catcgttttc gaggagcgaa gggtcaacca ccagttcaag
     6361 ttcagggttg aaggagaacg gcagaatcgt gccggtgacg ctgcccgcca acctttcggc
     6421 cgtttcttgc gaggcaaacg ccacgtaggt gccccccatg agcgccttga cggccgccag
     6481 gtctacccgc ctgtcgccgg gcacgaccgc aagcacgtac cgggtgactt tcttgccgat
     6541 cttgaccatg agcacgatac atttggctgc ctgcgagaga gtgttccctc gcatcgcgct
     6601 caccagttcg gtctgcccct ccggcgggtg atctatcaag cggtagggca ctccgcccgc
     6661 gttaaggtag gcaatgaagc gcgtgtaagg ctcctccgaa tccacagctt acctccgtag
     6721 cagctatata gatgtttggc agaaaagaat ggtagtgata taatcctgcc acgaattgct
     6781 gacgtttgcc agtagtagaa gtgacaagga gcatcaggaa attgccatgc acacagtcgc
     6841 gaccgtcgca tacgacgccg tcaacccctt tgagttggcg gtggcaacag aagtcttcgg
     6901 cttcgagcgt cccgagctag gggtgccctg gtacggcttt ctgatatgcg gcgtcgagcc
     6961 caggccgatc cgcacctcgg tgaacatgtt tctcagcacg ccctattcgt tacaacacct
     7021 cgcgcaggcc gataccgtga ttgtgcccag ttcgcgccca ggggtcgtgc ctgtgcccga
     7081 ggccctgctg gccgccctgc gtcaggctta cgagcgtgga gcgcgcatta tagccttttg
     7141 caccggggcc ttcatacttg cggaagcggg gctgctcacg gggcgccgcg tgaccacaca
     7201 ctgggcgtgg gcggcagagt tagccgagcg ttatccgggc gtgcgcgtcg atcccatggg
     7261 actctatatc gatgacggcc aggtgctgac ctccgcgggg acggccgccg caatcgacct
     7321 gtccctgcac atcgtgcgca aggactacgg ggccgacata gcagccgcag tggcgcgcag
     7381 gatggtcgtg ccgccgcacc gcgatggcgg gcaggcgcaa tacatcgaga cgccactctt
     7441 cactacttca gcccaggaag aaccgttcgg cacgaccctc agctgggtcg cgtgccacct
     7501 acacctggac ctctccgtgg agcagatagc agagcgggct atgatgagtc cgcgcacctt
     7561 cgcgcgccgt ttccgttcca ctctcggcat cacgccatac cggtggctta tacaacaacg
     7621 aattgtgctc gcacaacgac tgctggaaac gacgaccgaa cccatcgagc aggtcgccga
     7681 acgttgtggc ttcggttccg cagccgccct gcgcctgcac ttccaacgcc ttctccatat
     7741 ctctccccag acttaccgca aggcttttca gcacgaggag ggtaaagtgg ctagtggcta
     7801 gtggctagtg gctagtggct agtggttagt ggctagtggt tagtggctag tgattagtgg
     7861 tcagtggtca gtcggcagtg gtcaggggga gaaccccgcg gcgtcatccc tcctgaccac
     7921 tgaccactca ccactaaccc tgaccactgt cggctgaccg ctggccactg accactggcc
     7981 actaacctga ccactgtcgg ctgaccactg gcgactgacc actctgcccc cgccactcga
     8041 cggcttccgc ggggaaatcg gtgatgacgc cgtcgcagcc gatctcgcgc aggcgttccc
     8101 agtcgcgtgt gcggttggcg gtccagggga cgaccttaat cccgcgggcg tggagggagg
     8161 cgacgagggc ctcgtcgacg tagtggaagt tggggaaaca ccaggtggcg cctaggcggg
     8221 aggcgtaggt ggcgaggtcg aggaggtagc cgtagacggt gatccccaac gggatcccgg
     8281 tgttccggcg cgccacctcg gcgatcgtcg aatggtcgaa ggaggcgagg acgacgttcg
     8341 gccagccggc gatgtgctcg aggagcacgt cctcccattt gctgcgtttg acctcgagga
     8401 tcatggtcgt ccgctcggcg tacgcggcga ggtcgtggag ctgctggacg acggtgccgc
     8461 gctcggcgat ctcgacggcg gaaagggtct ccacgtcggc gtccgcgaag tcgtcatcgt
     8521 ggtacaggac ggcggtcttg tcggagagca gacggaggtc ggtttcgaag ccgtcggcgc
     8581 cgctccgcag ggcctcgtcg aacgacgcga tcgtgttctc cggaaagcgt ttcgggctgc
     8641 cgcgatggcc gaagatcagg aagggctccg ccatgcgtaa tgtcttgttc ccctgtactt
     8701 aacgtggtag gttgctgcga acatcggagc gaaaattcat ggcaaaaaaa atcctgctgg
     8761 cggatgacag tatcactatc cagaaggtgg tcgaactgac gttttcggat ggtgattacg
     8821 aggtcaccgc ggtcaacaac ggggccaagg cgatcgccaa gctcgcggac atgcggccgg
     8881 acatcatcct ctcggacatc atcatgccgg agaagaacgg ctacgaggtc tgcgaatacg
     8941 tcaaatcgca tccggaatac cgcaacatcc ctgtcgtcct tcttaccgga accttcgagc
     9001 cgttcgaccc tgatcgcgcc g
//
LOCUS       DASRWZ010000617         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1351420, whole genome shotgun sequence.
ACCESSION   DASRWZ010000617 DASRWZ010000000
VERSION     DASRWZ010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351420"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VFH60_09790"
     CDS             <1..1338
                     /locus_tag="VFH60_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HET6314116.1"
                     /translation="GAWWFVRYLLDGTRMPMPAVQVLAGTAGLVAAILVQAATNSPTN
                     GDYSLEWLLSLVYALLVSLVPWFLGGYRASERPDFNAAYAGFRIGLILIGFAAIMATI
                     IGPEAVAAAWVELGSVLLWFFVWALAGLAVGNRQMVREEYGGIKMGGWGSVLTLSIGA
                     ILIVGLIGGAANAGGLLGGVFALVVGALVLLGAVLYGLSFVLIMVVYYVFMFFAAIGS
                     AIGIGGGEQQQPQEASDGPGYASRFLERIRRDWEQGTWDLPPEVLTFIAWAGVLLGAV
                     IAILVVSSKLRRTARMREREVAEERESIGSWALLLQQIKEWLLRLFGRKGKGAEGVKG
                     SLEDDLAALARHAEWSGTLSVRQIYARMQAAAGKMGYPRAVQQTPIEYLSVLSNAMPD
                     LRAEFRDITAAYLEARYGPAPASDPAVQAASRAWKRAEAVMRPGVRGQGSGVR"
     gene            complement(1331..>2111)
                     /gene="argF"
                     /locus_tag="VFH60_09795"
     CDS             complement(1331..>2111)
                     /gene="argF"
                     /locus_tag="VFH60_09795"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET6314117.1"
                     /translation="MIFQKPSLRTQVSFETGMAQLGGHAVYLGPDHIALGKRESVPDV
                     ARVLGRMVDGIMARVFAHNDVETLAEYAGVPVINGLSDDEHPCQALTDLFTIYEKKRR
                     VEGVNLTYIGDGNNVAASLLLAGAKVGMNVTLISPEGYEVKTQYVKKARADAAISGSR
                     LKFSSDPDDVAGSDVLYTDTWASMGQESEAEQRIRIFQPYQVNAQLLRRTGTNTFVMH
                     CLPAHRGQEITDEVMDGSSSAVFDEAENRLHVQKALMALII"
BASE COUNT          354 a          577 c          752 g          428 t
ORIGIN      
        1 ggggcgtggt ggttcgtgcg ctacctgctg gatggcacgc gcatgccgat gccggctgtg
       61 caggtgttgg cgggcacggc cggtctcgtt gcggctattc tcgtgcaggc agcgacgaat
      121 tcgcccacga acggtgatta ctcattagag tggctgctct ccctggtgta tgcgctgctg
      181 gtgagcctgg tcccctggtt cctgggaggc tacagggcgt cggagcggcc cgacttcaac
      241 gcggcgtatg cggggttccg catagggctt atcttgatcg ggttcgccgc catcatggct
      301 accataattg ggccggaggc tgtagcggct gcgtgggtgg agcttgggag cgtgctgctg
      361 tggttcttcg tgtgggcgct ggccgggctt gcagtgggca accgccagat ggtgcgcgag
      421 gagtatggcg gcatcaagat gggcggttgg gggagcgtcc tgaccctcag cataggcgcg
      481 atacttatag tggggctgat aggtggcgct gcaaacgcag gtggtctcct gggaggcgta
      541 ttcgcgctgg tagtgggggc gctcgtactg cttggggcgg tcctgtatgg cctctcattt
      601 gtccttatca tggttgtgta ttacgtcttc atgttctttg ccgctatagg gagcgccata
      661 gggataggtg gaggcgagca gcagcagccg caggaagcaa gcgatgggcc tggttacgcc
      721 tccaggttcc tggaaaggat caggagggac tgggaacaag ggacgtggga cctgccgcct
      781 gaagtgctga ctttcattgc gtgggcgggc gtgctgttgg gagccgtgat cgcgatactg
      841 gtggtcagca gcaagctgag acgcacggcg cggatgagag agcgagaagt agcagaggag
      901 cgcgagagta taggttcgtg ggcgttgctg ttgcaacaga taaaggagtg gttactcagg
      961 ctgtttgggc gcaaaggtaa aggcgcggag ggtgtaaagg gtagcctgga agatgacctg
     1021 gcggcccttg caaggcatgc agagtggagc ggcacgctgt cggtgcgcca gatttatgcg
     1081 cggatgcagg cggcggcagg gaagatgggc tatccacgcg cggtccaaca aacgccgatt
     1141 gagtacctat ctgtgctgtc caacgcgatg cccgacctgc gagccgagtt ccgggacatc
     1201 acagccgcct accttgaagc gcgctacggc cccgcgcccg cctcagaccc ggcggtgcag
     1261 gcagccagca gggcatggaa gcgagcggag gcggtgatga gaccaggggt caggggtcag
     1321 gggtcagggg ttagataatc agcgccatca acgctttctg cacatggagg cggttttcgg
     1381 cctcatcgaa taccgctgat gaggagccat ccattacttc gtcggtgatt tcctggccgc
     1441 gatgcgccgg gaggcagtgc atcacgaagg tgttggtgcc ggtgcgcctg agcaattggg
     1501 cgttcacctg gtaaggctgg aagatacgaa tgcgctgctc ggcctcgctc tcctgtccca
     1561 tgctggccca ggtgtcggta tagagcacgt cgctgcccgc aacgtcgtcg gggtcgcttg
     1621 agaacttgag gcgcgagcct gatatggcgg cgtcggcgcg ggccttcttc acatattgcg
     1681 tctttacctc gtacccctcc gggctgatga gcgtgacgtt catgccgacc ttcgcacccg
     1741 ccagcaggag cgatgcggcc acgttgttgc cgtccccgat gtacgtcagg ttgacgccct
     1801 ccacgcgccg cttcttttcg tagatggtga agaggtcggt aagcgcctgg caggggtgct
     1861 cgtcgtccga caggccgttg atgaccggca cgcccgcgta ctccgccagc gtctcaacgt
     1921 cgttgtgggc gaacacgcgg gccatgatgc cgtccaccat gcgccccagg acgcgcgcca
     1981 cgtcgggcac gctctcccgc ttgccgagcg cgatatggtc cggccccagg tagacggcgt
     2041 gccccccaag ctgcgccatg ccggtctcga agctcacctg cgtcctgagc gagggcttct
     2101 ggaaaatcat c
//
LOCUS       DASRWZ010000618         4381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1284069, whole genome shotgun sequence.
ACCESSION   DASRWZ010000618 DASRWZ010000000
VERSION     DASRWZ010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284069"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1311)
                     /locus_tag="VFH60_09800"
     CDS             complement(106..1311)
                     /locus_tag="VFH60_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6314118.1"
                     /translation="MFKSRRFIIGLVISLVFLAWALSQEDLGKVFATIAGMNWVSLVP
                     ALALYFAGVWVRAVRWRIRLRPLRPNLPLFKVFDIVVIGYMANNVLPARIGELVRAYV
                     LSKRENVRKTSTLATIVVERIFDALVMVGFVAAALLFVIFFDADLLSTGEGHKFGTFI
                     TEWSPYIALGAAGFLAFVVFFVVTATSQRRVEGLIRFGLRFVPGKLHERVERLAGAFV
                     LGLGSLRSRTNMFLALVLSVTAWLLEAGMYYVIGNMGFNLVGSDGAAVPFYAYVLTTG
                     IVNLSTLIPQAAGYWGVFEAVSKVVLVGAIGVDSGLTTSYVILLHAALYFPVTLLGVV
                     FLARESLSWRELTDLEKQRVEASEAAHELEGPVTDVELASEGKLPDQGADRPVPDSPE
                     RISDPAKGR"
     gene            complement(1375..2253)
                     /locus_tag="VFH60_09805"
     CDS             complement(1375..2253)
                     /locus_tag="VFH60_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6314119.1"
                     /translation="METKLIDAAQGRDGRARKPRVVVVMPAYNAARTLAKTHGDLPKD
                     LVDKAILVDDASQDDTATIARQLGLDVIVHVQNKGYGGNQKTCYIEALNQGADIVVML
                     HPDNQYDATRIPAMIAPILEGRADLVLGSRLLSGRAATLKGGMPLWKYVSNRFLTIVE
                     NFSLGTSLSEAHTGFRAYSRKLLTTIPFLLNSDDFVFDSEVIAQTVAFGFKIAEVPVP
                     TRYFPEASSVGFRRSVIYGLATLNVSRKYWLHRKGIRRYPIFRRRLASVLSREYKERI
                     FGAERLPGEDRVGTRT"
     gene            2782..>4381
                     /locus_tag="VFH60_09810"
     CDS             2782..>4381
                     /locus_tag="VFH60_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314120.1"
                     /translation="MRVPRWETASIFAVTFAVLALFAPRIMTYLNPVTGDEPFYLMTA
                     ISIWKDGDLNECNNYRQLDEAAFHPPFYDPWYGYPVGWKGWVPTPYPLPPHPARLVPA
                     SRRCISTDPSVPLPANGTRSELYSKHGLGLSVLILPAFVAGGRELVVYFLVAMGALLA
                     ANVYLLARESAKQVWPALLTWLAFAFTVPQLPYSYLIFPELPAALFILYAFRRIRLWN
                     NNLFQVAAIGTAIAFLPWLHYRFVPVSAALFIYFVYNTLKRATPHRLASYALVVSQSL
                     VSAGLLMYFFYYRYQQPFPNVSDHAGSSDVAGTLRGAAGLFIDVQWGLFVAAPVYILA
                     IVGVALMGVRKPHRRDLLWLGLVFVPYFGVIANYAQFWGEWCPPARYLASILPLLAMP
                     FAFSLDRIRNWGFYAIYGVLFAVSVAVVAGYLYAPQWMYHHPEAYSLNNVITMGLPML
                     LRELPSGLQGIIDPNALNASLPSFVLPYFYYLQDRAYGDYTSALAWSRSIVPACVIGG
                     IVGLSLGLAWWSNRPRRATAGTALR"
BASE COUNT          831 a         1452 c         1246 g          852 t
ORIGIN      
        1 ggatgagacc ggcaagaact cggtgagtcc ctgcaccagc cccaggacta gcgcctgaaa
       61 tatgtccaag tagtattacc gttcctcatg agaattcttc acggcttagc ggcccttcgc
      121 cgggtcgctg attctttcgg ggctatcagg cacggggcgg tccgcaccct ggtccggcag
      181 cttaccttcc gacgccaact ccacatcggt gacaggacct tcaagctcgt gcgccgcctc
      241 ggaagcctca acccgctgct tttccagatc ggttagctcc cgccacgaca ggctctcgcg
      301 ggccaggaag acgacgccaa gcagcgtcac cgggaagtag agcgcggcgt gtagcaggat
      361 cacgtaagag gtggtaagcc cgctgtccac accaatagca cccacgagca cgactttcga
      421 gaccgcctcg aatacacccc agtaacccgc tgcctgtgga atgagcgtgc taaggttaac
      481 gatgcccgtt gtgagcacgt atgcatagaa cggcaccgcg gcaccatccg aacccacgag
      541 attgaagccc atgttgccga tgacgtagta catgcctgcc tctaacagcc aggccgtaac
      601 tgacagcaca agcgccagga acatattggt gcgcgagcgc aaggagccta gccccagcac
      661 aaaagcaccg gccagtcgct cgacccgctc gtgcagcttg cccggcacga aacgcagacc
      721 gaacctaatc aacccctcca cacgccgttg ggaggtggct gtcacgacga agaagaccac
      781 gaatgccagg aaccccgccg cccccagggc gatatagggg ctccactcgg taatgaaggt
      841 accgaacttg tgcccttcgc ccgtgctgag tagatcagcg tcgaagaaaa tgacgaagag
      901 gagggccgcg gccacaaagc ccaccatgac cagagcgtcg aagatgcgct cgaccacgat
      961 ggtcgccaac gtggaggtct tacgtacgtt ctcgcgcttg ctgagcacgt atgcgcgcac
     1021 aagctcacca attcgggcgg gtagcacgtt attagccatg tagccaataa caacgatatc
     1081 aaatacctta aagagtggca ggttaggtct cagtgggcgg agccgtatgc gccagcgcac
     1141 tgcccgcacc catacccccg cgaagtataa cgcgagtgcg ggtacgagcg agacccagtt
     1201 catccccgca atcgtggcaa agactttgcc caggtcctcc tgggagagcg cccaggcgag
     1261 gaagaccagg cttatcacca ggccgattat aaagcgccgt gacttgaaca aaatgatggc
     1321 tccggtggag gtatatatcc gtagaccgtg agaatggccc tacttattac gaggctacgt
     1381 gcgggtgccc actctatcct cgcccggcag gcgctctgcc ccaaatatgc gctccttgta
     1441 ctcgcgactc agcacgctcg caagccgcct gcggaatatg gggtagcggc ggatcccctt
     1501 ccggtgcagc cagtacttgc gcgacacgtt gagcgttgct agcccgtaaa tcacgctgcg
     1561 cctgaagccg acgctcgagg cctccgggaa gtagcgagtg ggcaccggca cctcggctat
     1621 cttgaagccg aaagccaccg tctgcgctat tacttcgctg tcgaacacaa agtcgtccga
     1681 gttgagcagg aacgggatgg tggtgagcaa tttgcgggaa taggctcgaa aacccgtgtg
     1741 cgcctccgac agactcgtgc cgagggaaaa gttttcgaca atcgtgagga acctgttcga
     1801 gacgtacttc cagagcggca ttcctccctt caaggtggcc gcgcggccgc tcaagaggcg
     1861 agacccgagc accaggtcgg cccgaccttc aaggatggga gcaatcatgg ccgggatgcg
     1921 cgtggcgtcg tactggttgt cggggtggag catcaccacg atgtccgcgc cctggttcag
     1981 cgcctcgatg tagcaggtct tctggttgcc gccgtagccc ttgttctgca cgtgcacgat
     2041 cacatccagg cccaactgcc gggcgatagt ggccgtgtcg tcctggctcg cgtcgtccac
     2101 caggatggcc ttgtccacaa ggtccttcgg caggtcgccg tgggtcttcg ccagcgtgcg
     2161 ggcggcgttg taagcgggca tgactactac cactcgcggc ttgcgggcac gtccgtctct
     2221 gccctgagcg gcgtctatca acttggtctc catttgcctc cccgtctgcc tggcatcctt
     2281 gcatgccttt cagccgtgga attgtagcac acgtcagacg acccacgcaa atgtgctcgc
     2341 ctacgcgtca tatagcctcc cctcgccctg tggctgaagc cacgggctga caaggggcaa
     2401 agtcccctgc ggggactagg gaacatcatg tagtctccgc aggagacttc gtctatggtc
     2461 agcccgccaa tttattggca gggcgtcccc gcagggcgaa cgtgacgcgg ccctcaccta
     2521 gattgatctg gtcctgcccc acgagcgaag cagttgacgc acaagcggcc gagtgataga
     2581 atcgagctaa tatgctgctt tgcgggcggc cagtacgctc cacttgcata tgcgcgtctc
     2641 tatctcgacg cgccacccac ccttttgcgc tcgcggtccc ccatgcagcc tccgattgcc
     2701 agcaccgata acacaagatt cctgctccgg cggcgtggtc cccgtcccac cgcgactcca
     2761 gcagaaagca ccgtatcgat cgtgcgcgtg ccccggtggg agaccgcttc gatcttcgcg
     2821 gtcacgttcg ctgtgctggc cctcttcgcc cccaggataa tgacctacct caacccggtc
     2881 acgggcgacg aaccgttcta cttgatgact gccatcagca tctggaagga cggcgatcta
     2941 aacgagtgca acaactaccg gcagctcgac gaggccgctt ttcacccgcc attctacgac
     3001 ccctggtacg gctacccggt aggctggaaa ggctgggtcc ccacacccta cccgctgccc
     3061 ccgcacccgg caaggttggt gcccgcaagc cgcaggtgca tcagcacgga ccccagcgta
     3121 cccctacccg ccaacggcac caggagcgag ctttattcca agcacggcct cggcctgagc
     3181 gtgctcatac tacccgcgtt cgtggccggg ggtcgagagc ttgtcgtcta cttcctggtg
     3241 gcgatggggg cgctgctcgc ggcgaacgtc tacttgttgg cccgcgagag cgcgaaacag
     3301 gtctggcccg cgctgctcac atggctggct ttcgccttca cggtgccaca attgccctac
     3361 tcctacctta tcttcccgga gctaccggcg gccctcttca ttctgtacgc cttccgccgc
     3421 atacgcctct ggaacaacaa cctgttccag gtggcggcaa tcggcaccgc tatcgcgttc
     3481 ctaccctggc tgcactaccg cttcgtcccg gtaagcgccg cgctcttcat ctacttcgtt
     3541 tacaacaccc tcaagcgcgc aaccccgcat cgactcgcga gctacgccct cgtggtcagc
     3601 cagagtcttg tctcagcggg cctgctgatg tacttcttct actacaggta ccagcagccg
     3661 tttcccaacg tctccgacca cgcgggtagc agcgacgtgg cgggcacgct ccggggcgca
     3721 gccgggctgt tcatagacgt gcagtggggc ctcttcgtcg ccgcgccggt ctacatcctg
     3781 gctatcgtgg gtgtggccct catgggtgtc cgcaagccgc atcgccgcga ccttttgtgg
     3841 ctggggcttg tattcgtgcc ctacttcgga gttatcgcca actacgcgca gttctggggc
     3901 gagtggtgcc cacccgcgcg ctacctcgcc tccattctgc cgttgctggc tatgccgttc
     3961 gccttctccc tcgaccgcat acgcaactgg ggattttacg ccatctacgg tgtgttgttc
     4021 gcggtgagtg tggccgtggt cgcggggtac ctgtacgcgc cccagtggat gtaccaccac
     4081 cccgaagctt acagcctgaa caacgtcatc acgatgggcc ttcccatgct cctccgagaa
     4141 ctgccgagcg gcctgcaagg catcattgac cccaacgccc tcaacgcctc gctcccctct
     4201 ttcgtgctgc cctacttcta ttacctgcaa gaccgcgctt atggtgacta cacttccgcg
     4261 ttggcgtggt caaggagcat cgtccctgct tgcgtcatcg ggggaattgt cgggctgagc
     4321 ctgggccttg cctggtggtc gaatcgcccc cgacgtgcca cggcggggac cgccctcagg
     4381 c
//
LOCUS       DASRWZ010000619         5189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_324227, whole genome shotgun sequence.
ACCESSION   DASRWZ010000619 DASRWZ010000000
VERSION     DASRWZ010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324227"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFH60_09815"
     CDS             complement(<1..801)
                     /locus_tag="VFH60_09815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6314121.1"
                     /translation="MSTDKYDPRYVSRFYDNYGDREWQRFEQEAGPANLANLHLHTHY
                     LRRFIRPGDDVLDIGAGPGRFTIEMARIGARITVGDISPVQLEMNRQKVEEAGLQGAV
                     VARQVMDVTDLSPLPSATYDSAVCYGAVLSYLFDRAGDALEELLRVTKPGGYVLLSVM
                     SLLGGTRAFLPGVLKLTEQLGVEVVYQVNSTGDLDGEVVGGHKAHMYRWSELEALLQA
                     HHCEIVAASASNFLPRQHPEAVQQAMSDPQTWEARLDWELDFCKEPGAI"
     gene            complement(809..1207)
                     /locus_tag="VFH60_09820"
     CDS             complement(809..1207)
                     /locus_tag="VFH60_09820"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET6314122.1"
                     /translation="MTYASLNDGFNDILAACSPKVRDLASQARALIRDVMPDVVEVPW
                     PTQGIAGYGVGPKKMSEHFCYISTHKDHIDLGFNYGSELPDPEGLLAGTGKLFRHIKI
                     NTPEDLARPAVLRLLEVASTHRMPSKAAGE"
     gene            1466..2527
                     /gene="wecB"
                     /locus_tag="VFH60_09825"
     CDS             1466..2527
                     /gene="wecB"
                     /locus_tag="VFH60_09825"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET6314123.1"
                     /translation="MKVLSVVGARPQFIKAAPVSRAMTRAGIEEVLLHTGQHYDPAMS
                     EVFFAELDIPAPRHNLGVGSGSHAGQTAAMLVGIEAVLLDERPDYLLVYGDTNSTLAG
                     ALAAAKLGVPVAHVEAGLRSYNRDMPEEINRVVADSLSSLLFCPTGVAAANLEREGIT
                     RGVFVVGDVMYDAILDSIPRAEGIAPSLLSKLGVESGSYLLATVHRASNTDDSANLAN
                     IVRALSDSAEPVVFPAHPRTARALQQAGITPGPNVRQIGPVSYLEMLALEKHARKVLT
                     DSGGVQKEAMWLSVPCITMRSETEWVETVELGWNTLTGTDPARILAAIHAPRLHSTPP
                     DVYGDGHAAENIVNVLRNT"
     gene            2637..3716
                     /locus_tag="VFH60_09830"
     CDS             2637..3716
                     /locus_tag="VFH60_09830"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6314124.1"
                     /translation="MINIARPMIGEEEKAAVIAVLESGQLAQGGVVAEFERAFADYCG
                     VKHAIATSNGTTALHVAVLAHDLGPGDEVITVPFTFIASANSVLYSGARPVFVDIEPD
                     SFNMAMEQVEAAITPRTKAIMPVHLFGNMAEMSRLTEIAERHGLIVIEDAAQAHGSEE
                     GGRRAGSWGTGCFSFYPTKNITTGEGGIVTTNDDCVADRARLIRSHGMRVRYYHESLG
                     FNFRMTNIHAAIGLAQLPKLESFNARRIENAAYLSERLPSDKVQVPTVRPGTRHVFHQ
                     YTVRVLPPLDRDDVRKQLADAGVGSEVYYPVPVHQQQFYTDQGYSGSFPESERAAREV
                     LSLPIHPGLTQQDLDTIVDAVRDLG"
     gene            3719..4762
                     /locus_tag="VFH60_09835"
     CDS             3719..4762
                     /locus_tag="VFH60_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6314125.1"
                     /translation="MTTHPRLADADSRPPKLAVIGAGSMGRNHLRVLNDLDTVSLVGV
                     ADADIASAERAARRFHIPAYGSYLDMLDRERPDGLIVAVPTVLHRQVTLEAIARGAHV
                     LVEKPIAFTVDEGREMIEAARHAGVVLTVGHIERYNPAILELHKRLHEQQLGRVFQMN
                     ARRLGPFPPRVRDVGVVIDLATHDVDIMRFLSGSEVVRVQAETARRIHTEHEDLLSGL
                     LRFANESIGVLDINWLTPTKIRELMITGERGTFQVNYLTQDLYFYENNYAKTDWDTIS
                     NIEGVSEGDMVRIRIEKTEPLRVELDRFARAVRGEEVSIVSGEDGLRALHVARKIVES
                     GLVGRTIYMDDEG"
     gene            4795..>5189
                     /locus_tag="VFH60_09840"
     CDS             4795..>5189
                     /locus_tag="VFH60_09840"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6314126.1"
                     /translation="MAVVGLGKIGLPLAAQFARAGLEVIGCDISPEVVATVNAGRSHV
                     REEPGLEELVAEMVREGRLTATTGTPGAVSRCNVVVIIVPLMVDEERNIDYRAIDSAT
                     RSVGQGLQRDTLVIYETTLPVGTTRTRLGP"
BASE COUNT          961 a         1677 c         1630 g          921 t
ORIGIN      
        1 gattgcccca ggctctttgc agaagtccag ctcccagtct agtcgggctt cccaggtttg
       61 ggggtcggac atggcctgct ggacggcctc cgggtgctga cggggcagga agttggaggc
      121 cgaggccgct actatctcgc agtggtgggc ttgcagcaga gcttctagtt cggaccacct
      181 gtacatgtgg gccttgtggc cgcccaccac ctcgccgtcg aggtcgccgg tgctgtttac
      241 ctggtagacc acttcaaccc ctagctgctc ggttagcttg agcacgcccg gcaagaaggc
      301 gcgagtgccg ccgagcaggg acatcacgct cagcagcacg taacctcccg gcttcgtcac
      361 ccgcagtagc tcctccagtg catctcctgc cctgtcgaac aagtagctga gcacggctcc
      421 gtaacacacc gccgagtcgt aggtggcgct cggcaggggg gacaggtcgg tgacgtccat
      481 cacctgccgc gccaccaccg cgccttgtaa cccggcctcc tctaccttct ggcggttcat
      541 ctcaagctgg acgggagaaa tatcgcccac ggtgatgcga gcgcctatcc gcgccatctc
      601 tatggtgaaa cgccccggac cggcacctat gtctagcacg tcgtcgccag gccgtatgaa
      661 gcgccggagg tagtgggtgt gcaggtgcag gttggcgagg ttggcggggc ctgcctcctg
      721 ctcgaaccgc tgccactcgc ggtcgccgta gttgtcgtag aagcggctga cgtacctggg
      781 atcgtacttg tcggttgaca tgttggttct actctccggc cgctttggac ggcatgcgat
      841 gggtggaggc aacttcgagc aaccggagca cggcggggcg ggccaggtcc tccggcgtgt
      901 tgatcttgat gtggcggaag agcttccccg tgcctgccag caagccctcc gggtcgggta
      961 gctcgctgcc gtaattgaag cccaggtcga tgtggtcctt gtgcgtgctt atgtagcaga
     1021 agtgctccga catcttcttc ggccccaccc catagcctgc tatcccctgc gtgggccacg
     1081 gcacctcaac cacatcgggc atcacatccc tgatgagtgc ccgcgcctgc gaagccaggt
     1141 cccgcacttt tgggctgcaa gccgccagga tgtcgttgaa gccgtcgttc agcgatgcat
     1201 aagtcatatt attctccttt gcgaacgaac tctcctgcca ggtgactcca tcctataggt
     1261 acctctctac aggacacgta cggcacgcca caagtctatg ccaatccgca tgccgtgaca
     1321 aatacggcaa ctgaacattg caaccatctc aacctcacct cccacttaag gatgtgtcgt
     1381 gggaggtgag gttgtgcgtg cgctttgctt gccctgcttc gtgggtgtta taataagcgt
     1441 tggctctcga tgggcctgtt gcattatgaa ggtactctct gtagtagggg cgcgacccca
     1501 atttatcaag gccgctcccg tgagccgggc catgacgcgg gccggtatcg aggaagtgct
     1561 gctgcacacg ggccagcact acgacccggc gatgtccgag gtcttcttcg cggaactgga
     1621 catacccgcc ccccggcaca acctcggcgt aggctcaggc tcccacgccg ggcagaccgc
     1681 cgccatgctg gtgggcatcg aggcggtgct gctcgacgaa cggcccgact acctgctggt
     1741 ctacggcgac accaactcga cgctggcagg cgcactggcg gcggccaagc tgggtgtgcc
     1801 ggtagctcat gtcgaggctg ggctgcgctc ctacaaccgc gatatgccgg aggagatcaa
     1861 ccgagtggtg gccgacagcc tctccagcct gctattctgc cccacgggcg tggccgccgc
     1921 caacctggag cgagaaggca tcaccagggg cgtgttcgtg gtaggtgacg tgatgtacga
     1981 cgctatactg gactccatac cacgagctga aggcatagcg ccctcgctgt tgtccaagct
     2041 aggggtagag agcggcagct acctgctcgc caccgtccat cgcgcctcca acaccgacga
     2101 ttcggcaaac ctcgcgaata tagttcgtgc cttgagcgac tccgccgagc ctgtggtctt
     2161 tcccgcacac ccccggaccg ctagggcgct gcaacaggcg ggcatcactc ccggcccgaa
     2221 cgtgcggcaa atcggcccgg tatcgtacct agagatgctg gcactcgaaa aacacgcccg
     2281 caaagtgctc accgactcag gcggcgtgca aaaagaggcg atgtggctgt ccgtcccttg
     2341 cattactatg cgcagtgaga ccgaatgggt cgagaccgta gagttaggtt ggaacaccct
     2401 caccggcacc gaccccgccc gtatcctcgc cgccatacac gccccacgcc tgcactcaac
     2461 gccaccagac gtatacggcg acggccatgc ggcggagaat attgtgaacg tgctaaggaa
     2521 tacgtgacgc atgccgagtc gcgcaccaaa actttccgaa tctttgcgcg ttctttgcgt
     2581 cctttgcgtc tttgcgtttt cgtaactcat aactcataac gggaaaggac acccacatga
     2641 tcaacatagc ccggcccatg ataggcgagg aggagaaggc ggcggtgatt gctgtgctcg
     2701 aatcggggca gctcgcgcaa gggggcgtgg tggccgagtt cgagcgggct tttgccgact
     2761 attgcggcgt caagcacgcc attgccacca gcaacggcac cacggccctg cacgtcgccg
     2821 tcctcgctca cgacctggga ccgggcgacg aagtgatcac cgtgcctttt accttcatcg
     2881 cctcggctaa ctcggtcctc tactcgggtg ccaggccggt gttcgtggac atagagccgg
     2941 atagcttcaa catggcgatg gagcaggtag aggcggctat caccccgcgc acgaaggcca
     3001 tcatgcccgt gcacctgttc ggcaacatgg ccgagatgtc gcgtctcacc gagatcgcgg
     3061 agaggcacgg cctcatcgtc atcgaggacg cggcccaggc gcacggctcg gaggagggcg
     3121 gacgtcgggc cggtagctgg ggtaccggct gcttctcctt ctaccccacc aagaacatca
     3181 ccaccggcga gggcggcata gttaccacca acgacgactg cgtagccgac agggccaggc
     3241 tcattcgctc ccacggcatg cgagtgcgct attaccacga gtcgctcggc ttcaacttcc
     3301 gcatgaccaa catccacgcc gcgattggcc tggcccaact gcccaagctg gagagcttca
     3361 acgcccgccg catagaaaac gccgcctact tgagcgagcg cctgccctcc gacaaggtgc
     3421 aggtgcccac ggtgcggccc ggcacccgcc acgtcttcca ccagtacacc gtgcgcgtgc
     3481 tccctcccct cgaccgggac gacgttcgca agcagttggc cgatgcgggc gtgggcagcg
     3541 aagtctacta ccccgtgccc gtgcaccagc agcagttcta cactgaccag ggctacagcg
     3601 gcagcttccc ggagtcggag cgggcggcgc gggaggtgct gtcccttccc atccaccccg
     3661 gcctgaccca gcaggacctc gacaccatag ttgacgccgt gcgggacctg ggctagcaat
     3721 gaccacccac ccacgcctcg cggacgctga ttcgcgcccc ccgaagctgg ccgtaatcgg
     3781 cgcaggctcg atgggccgca accacctgcg cgtgctcaac gacctcgaca ctgtcagcct
     3841 ggtgggcgtt gcggacgccg acatagcgag cgccgagcgc gcggcccgtc gcttccacat
     3901 acccgcttac ggcagctacc tcgacatgct cgacagggag aggcccgacg gcttgatcgt
     3961 ggccgtgccc accgtcctgc accggcaggt gacgttggag gcgatagcgc gtggagcgca
     4021 cgtgctggtg gagaagccga tagccttcac cgtggatgag gggcgcgaga tgatcgaggc
     4081 ggcgcggcac gcgggggtgg tcctcaccgt gggacatatc gagcggtaca acccggcgat
     4141 actggagctg cacaagcgcc tccacgagca gcagcttggc cgggtgttcc agatgaacgc
     4201 tcgcaggctg gggccgttcc cgccacgcgt gcgagacgtg ggtgtggtga tagacctggc
     4261 gacccatgac gttgacataa tgcgcttcct gagcgggtcc gaagtggtgc gcgtgcaggc
     4321 cgagaccgcc cgccgcatcc acaccgagca cgaagacctg ctgtcgggcc tgctccgctt
     4381 cgccaacgaa tcgattggcg tgctcgacat caactggctc acgcccacca agatacgcga
     4441 gcttatgatc accggggagc ggggcacgtt ccaggtgaac taccttacgc aagacctcta
     4501 cttctacgag aacaactacg ccaagacgga ctgggacacc atctccaaca tcgagggcgt
     4561 gagcgaaggc gacatggtgc gcatccgcat cgagaagacc gagccgctcc gggtagagct
     4621 tgaccgcttc gcccgtgcgg tccggggcga agaagtgtcc atcgtatcgg gcgaggacgg
     4681 gcttcgggcg ttgcatgtgg cccgcaagat cgtggagtcg gggctggtgg ggcggaccat
     4741 ctacatggac gacgaaggtt aagcttggag acagcgcgag aaagcagtta cagggtggcg
     4801 gtagtaggcc tcggcaagat aggtctgccc ctggcggcgc agttcgcgcg cgccgggctt
     4861 gaggtaattg gttgcgacat atcccctgag gtggtggcga cggtcaacgc gggtcgctcg
     4921 cacgtcaggg aggagccggg gcttgaggag ctggtagccg agatggtgag ggagggccgc
     4981 ctcaccgcca ccaccggcac gcccggtgcc gtcagccggt gcaacgtggt ggtaatcatc
     5041 gtgcccctca tggtggacga ggagcgcaac atcgactacc gcgccatcga ctcggccaca
     5101 cgctcggtcg ggcaggggct acaacgcgac acgctggtca tctacgagac caccctgccg
     5161 gtgggcacca cgcgcacgcg cctggggcc
//
LOCUS       DASRWZ010000620         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_530475, whole genome shotgun sequence.
ACCESSION   DASRWZ010000620 DASRWZ010000000
VERSION     DASRWZ010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530475"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1514
                     /gene="moeB"
                     /locus_tag="VFH60_09845"
     CDS             45..1514
                     /gene="moeB"
                     /locus_tag="VFH60_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013051199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET6314127.1"
                     /translation="MTTILIPTALRQYAGQQSEVSLQGGTVDEVLQELSTRYPDLRRQ
                     LYSESGKLRNFVNIYLNEDDVRYLQKGATPVSDNDTISIIPAIAGGAPHGAVLDTPPP
                     PVATGGEEQTLSHAEIRRYSRHLIVPEVGLEGQKKLKASSVLLIGAGGLGSPLALYLA
                     AAGVGRIGIVDFDIVDESNLQRQVIHGTSGIGTPKLESAKARILDINPNVQVDSYEYP
                     LTSENALEIFRPYDVIVDGTDNFPTRYLVNDACVLLGKPNVYGSIFRFEGQASVFWSE
                     HGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGTIGTIQATETIKLLLGIGEPLIGRLM
                     LYNALDMRFREVRLRKDPDCPVCGENPTVTELIDYQVFCGINAQTERGETDFDISPRD
                     LKSRLDAGQKLVLLDVREPHEWDITHLEGAKLIPVNQVTAHAHELSTADEMVVYCKAG
                     TRSARAVNELRQLGFRKLWNLRGGINAWAEDVDPSLPRY"
BASE COUNT          341 a          595 c          512 g          294 t
ORIGIN      
        1 accgtccaga acatatcaaa gcacccataa ggagaacatc ccacatgacg accatcctaa
       61 tacctacggc cctgcgccag tatgccggac agcagagcga ggtatctttg cagggaggca
      121 ccgtagacga ggtgttgcag gaactttcca cccgctaccc cgacctgcgc cggcagctat
      181 acagcgagag cggcaagctg cgcaacttcg tgaacatcta cctcaacgag gacgacgtgc
      241 gctacctcca gaagggcgct acccccgtct cggataacga cacgatctct atcatcccgg
      301 cgatagcggg cggtgctccc catggcgcgg tgctcgatac gcctccaccc ccggtagcca
      361 cgggcggcga ggagcagacg ctgtcgcacg cggagatacg ccgctacagc cgccacctca
      421 tcgtgccgga agtcgggctg gagggccaga agaagctcaa ggcaagcagc gtgctcctga
      481 ttggggcggg cggcctcggc tctccacttg ccctctacct ggcggcggcg ggcgtggggc
      541 gcatcggcat cgtggacttc gatatcgtgg acgagtcgaa cttgcagcgc caggtgatac
      601 acggcacgtc gggcatcggc acccccaagc tggagagcgc caaagcccgc atcctcgaca
      661 tcaacccgaa cgtgcaggtg gacagctacg agtaccccct cacctcggag aacgcgctgg
      721 agatattccg cccctacgac gtaatcgtgg acggtaccga taacttcccc acccgctacc
      781 tggtgaacga cgcctgcgtg ctgctgggca agccgaacgt gtacggctcg atcttccggt
      841 tcgaggggca agcgtcggtc ttctggtctg agcatgggcc gtgctaccgc tgcctctacc
      901 ctgagcctcc tcctcccggc ctggtgccct cctgcgccga gggcggcgtg cttggcatcc
      961 tccccggcac gattggcacc atacaggcca ccgagactat caagctgctg ctgggtatag
     1021 gcgagccgct gattggccgc ctcatgctgt acaacgccct cgacatgcgc ttccgcgagg
     1081 tacgtctgcg caaagacccc gactgcccgg tgtgcggcga gaaccccacg gtcaccgaac
     1141 tgatcgacta ccaggtgttc tgcggcatca acgcgcagac cgagcgcggc gagaccgact
     1201 tcgacatctc cccccgcgac ctcaagtccc gcctcgacgc gggccagaag ctggtgctcc
     1261 tcgacgtgcg cgagcctcac gagtgggaca tcacccacct ggaaggggcg aagctcatcc
     1321 cggtcaacca ggtgaccgcc cacgcccacg agttgagcac cgccgacgag atggtggtct
     1381 actgcaaagc cggcactcgc tcggcccgcg cggtgaacga gctgaggcag ctaggcttcc
     1441 gcaagctgtg gaacctgcgc ggcggcatca acgcctgggc cgaggacgta gacccttccc
     1501 tgccccgtta ctaatcacca ctagaattag acggaaccgc ccagacgcca agagcgcgcc
     1561 aagattatgg atggtgttct aaactagagg accagggtac aacaacacgt accctggtcc
     1621 tcttagtacc accttccctg acctcagcgc gtcctctgcg tctctgcgtc tctgcggcgg
     1681 accgttctta acttggcgtg gttattggcg tgcttggcgt cccttcggct aggctcaggg
     1741 ca
//
LOCUS       DASRWZ010000621         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_119315, whole genome shotgun sequence.
ACCESSION   DASRWZ010000621 DASRWZ010000000
VERSION     DASRWZ010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119315"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFH60_09850"
     CDS             <1..531
                     /locus_tag="VFH60_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6314128.1"
                     /translation="LYRRNFDLVEMCGRVVAEYQQSARRHQLVLETAVPRLVGQWDPM
                     RIERVLDNLISNAIKYSPEGGPISIEIGGESAAGGGDGVGHDRVTLVVRDQGVGIPEA
                     DVPYIFDWFRRARNVTGRIRGTGIGLAVVRQVVEQHGGTIAVESREGEGSAFTIELPL
                     QVPEATPTVEPESIEA"
     gene            complement(860..1384)
                     /locus_tag="VFH60_09855"
     CDS             complement(860..1384)
                     /locus_tag="VFH60_09855"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6314129.1"
                     /translation="MTHEDHVRLLQGGIPGPGGVWADLGSGGGAFTLALADLLGPAAT
                     IYSVDRDGGALREQEAAMRARFPDSAVHYIASDYTRAVEMPPLDGVVMANSLHYQRHK
                     EPVLQLVRGYLKPGGRFILVEYNTDHGNPWVPHPLSYPTWERLSLRNGFDTTQLLTTV
                     PSRFLREIYSAVSR"
     gene            complement(1405..>2593)
                     /locus_tag="VFH60_09860"
     CDS             complement(1405..>2593)
                     /locus_tag="VFH60_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314130.1"
                     /translation="TDSAETPTTPDEEGVVLTDPAATELQATVVAGLPPTPTPGGESS
                     GFGGFDGVNVFHLDTADGFGTLWVAYTYGMRSFEPLEQHFVAVYNQEETGWRELGRVE
                     LEDPDYLDSAGVQQVAVDMESTWLQVDSGVGAHSGCFNILRFDGQELHDELSHCGSAP
                     GSGSLEDLNGDDVMEVLLDATDHYVFCYACNVDYIVTDVVRWDGAQMVDVELEPLPDT
                     EPEELRQLTDRAVELAQHDLWKDAQEVIADTARFETQDETYTWNAALINLDASKRAAH
                     IQDSGYPLVSTVFYGDYPAVLDMLRGYTTEELFNPNSSLFTNTPAEGNTDFMAEYVTR
                     TTTLQLDAEPDLAGAYFVRGWSLALVNPRDPNALADLQQAADLDPSESLFSDSLTYLE
                     GQQ"
BASE COUNT          466 a          794 c          874 g          459 t
ORIGIN      
        1 ctataccggc gcaacttcga cctcgtggag atgtgcgggc gcgtggttgc cgagtaccag
       61 caaagcgcca ggcggcacca gctggtacta gagacggccg tgccccggtt ggtaggccag
      121 tgggacccca tgcgcatcga gcgagtgctg gacaacctga tctccaacgc catcaagtac
      181 agccccgaag gcggcccgat aagcatcgag attgggggcg agagtgccgc agggggtggc
      241 gatggcgtgg gccacgaccg ggtgactctc gtggtgaggg accagggggt aggcatcccg
      301 gaggcagacg tgccctacat cttcgactgg ttcaggcgcg cccgcaacgt cactgggcgc
      361 atacggggca caggcatcgg cctcgcggtg gtccgccagg tggtggagca gcacggcggc
      421 acaatcgcgg tggaaagcag agagggcgaa ggctcagcct tcacgataga gctgccccta
      481 caggtgcccg aagccacgcc taccgtcgag cctgagagta tcgaggcatg agtgataggg
      541 aagagacgga accgccaaga cgccaaggac gcctagaacg cgccaagata attaaagtac
      601 ggctcaccgc agaggcgcag agtagggtaa gggtttggca aatgtcacgt tgcgtagtac
      661 cctgcttctc ccttgtcatc ctgagcgtag cgaaggatca gccgcccccg tttgactctc
      721 cctactgaag ttccaatgtt cctctagccg acagcatccc cgatgttgaa gcctctggcc
      781 tacccacctg atccttcgct acgctcagga tgacaagggt ggcgccccgt cccaaaagat
      841 ggcggttccg tctctgcttc tatctgctta ccgccgagta gatttcgcgg aggaagcggc
      901 tgggcacggt agtgagcagt tgggtggtgt cgaagccgtt gcgcagggag agacgctccc
      961 aggtggggta agaaagcggg tggggcaccc acgggttgcc gtggtcggtg ttgtactcga
     1021 ccaggatgaa gcggcctccc ggcttgagat agccgcgcac gagttggagc accggctctt
     1081 tgtgacgctg gtagtgcagc gaattcgcca tgaccacgcc gtcgaggggc ggcatctcca
     1141 cggcgcgggt gtagtcggag gcgatgtagt ggacggcgct atcggggaag cgagcgcgca
     1201 tggccgcctc ttgctcccgc agcgcgccac cgtccctgtc caccgagtag atggtggcgg
     1261 caggccccag caggtcggcc agggcgaggg taaacgcgcc cccgcccgag ccgaggtcgg
     1321 cccacacccc gccggggccg gggatgccgc cttgcaacag tctaacgtgg tcttcgtggg
     1381 tcatggcagg ttacgcagcc ggggctactg ctgcccttcc aaatacgtga ggctgtcgga
     1441 gaacaaactc tccgaggggt cgaggtcggc ggcttgctgg aggtcggcaa gggcgttggg
     1501 gtcccttggg ttgaccaggg cgagcgacca gccccggacg aagtaggccc cggcaaggtc
     1561 cggctcggca tctagctgca aggtggtggt gcgcgtcacg tactcggcca tgaagtcggt
     1621 attgccctcg gcgggcgtgt tggtgaagag ggacgagttc gggttgaata gctcctcggt
     1681 ggtgtagccg cgcagcatgt ccagcaccgc cgggtagtcg ccgtagaaga cggtagaaac
     1741 tagagggtag ccgctgtcct ggatgtgcgc ggcgcgcttc gaggcgtcca ggttgataag
     1801 cgcggcattc caggtgtagg tctcgtcttg cgtctcgaac cgggccgtat ccgcgatgac
     1861 ctcctgcgcg tccttccaca ggtcgtgctg agcaagctcg accgcccggt cggtaagttg
     1921 gcgcaactcc tccggctcgg tgtcgggcag cggctcaagc tccacgtcca ccatctgcgc
     1981 gccgtcccag cgcaccacat cggtgactat atagtccacg ttgcaggcgt agcaaaagac
     2041 gtagtgatcg gtggcgtcca gcagcacctc cattacgtcg tcgccgttca ggtcctccag
     2101 gctgccggag ccgggggcgg agccgcagtg cgacagctcg tcgtgcaact cctggccgtc
     2161 gaagcgcagg atgttgaagc agccgctgtg cgcccccacg ccgctgtcca cctgcagcca
     2221 ggtactctcc atatcgacgg ccacctgttg caccccggcg ctatctaggt agtccgggtc
     2281 ctcaagctct acccggccca gttctcgcca gcccgtttct tcctggttat agacggccac
     2341 gaaatgctgt tcaagcggct cgaagctgcg catgccgtat gtgtaagcca cccacagcgt
     2401 tccgaaccca tccgccgtgt cgagatggaa aacgttcacg ccgtcgaagc ccccgaagcc
     2461 ggagctttca ccgccgggcg tgggagtagg ggggaggccc gccaccaccg tggcctgaag
     2521 ctcggtagcc gccgggtctg tcagcaccac gccttcctcg tcaggtgtag tgggcgtttc
     2581 ggcgctatcc gtg
//
LOCUS       DASRWZ010000622         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_941706, whole genome shotgun sequence.
ACCESSION   DASRWZ010000622 DASRWZ010000000
VERSION     DASRWZ010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941706"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFH60_09865"
     CDS             <1..184
                     /locus_tag="VFH60_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314131.1"
                     /translation="MSALALWRVPCGGRWRLIPLTKSNVRVGPRSTSESDWYGLPGKR
                     RQHLSQVARGLILTAR"
     gene            complement(171..1517)
                     /locus_tag="VFH60_09870"
     CDS             complement(171..1517)
                     /locus_tag="VFH60_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314132.1"
                     /translation="MTRRWSIVAATPGVAIMLFSAVALAQTPPPGTPTAGSSGTPTAV
                     PAGAGTVTPIPTAQLPETVYKAGGGPFAFANPFEGTGRMELLWVRTDRPIKEEKVART
                     WMWGPGPNTPGLLEQYNEDPSGRGQRLVQYFDKSRMEINDPHGNWNTDFYVTNGLLAG
                     ELVSGIIQVGEEDFIRYRPACIGMTGDFGDTLAPTYAAFKEVSNTVDGDRHAPNLTGH
                     RVTQTIDSTGKTGDDPAKSSVPGVDIAYFEARTGHNIPRVFWDFLNSSGEVYEIVLRG
                     DRLVSDEQLTSGRLSSPWYYAAGMPISEAYWAKASIRGETTDVLVQAYERRVLTYVPT
                     NGPGFQVEMGNIGQHYFDWRYRNAGLCPGGAPGTPTPPRPAPTGTIAATTPTIFSTPP
                     PLPTGSVIPAGTGTPPLPHTLAVPTGTITPLPLSTDTPAGTAPAGATGTPTPATVP"
     gene            1782..>2715
                     /locus_tag="VFH60_09875"
     CDS             1782..>2715
                     /locus_tag="VFH60_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6314133.1"
                     /translation="MQGGYTAISEREALVRFCARYTGDPTGAEDLAQKTLLAAWQHEQ
                     QLRDPQARQAWLFSIARRECLAWARSRDRSRLVNLDSLDEWEGQSRLAGDFDIETELE
                     RDDLARLLDRAMGLLPPDLREVLVRRYIEESSQGELAERLGITEGAVEARLHRGKLAL
                     RRVLSTKLSEDALAFGLISASDAGWEETRLWCPGCGRRRLEGRFRRDEGWLYMRCPDC
                     SRSGDDFSHFIHSTLSNKLRAFQSYKPALSRVLEGIHRMYRVQARRGATRCPGCGEWQ
                     AIGQGTPPWVPERFADPQSVYLYHPECGVYDWESW"
BASE COUNT          429 a          820 c          955 g          511 t
ORIGIN      
        1 aatgtcggcg ctagccctgt ggcgggtgcc ctgtggggga cgctggcgtc tcattcctct
       61 cacaaagtcg aatgtgcggg tcggtccgcg ttcgacctca gagagcgatt ggtacggcct
      121 gcccggtaag cgcaggcagc atctctccca ggtcgcacgc gggcttatac ttacggcacg
      181 gtagcgggcg tgggcgtgcc ggttgccccg gcgggggctg tgcctgccgg tgtgtcggtg
      241 gacagcggca ggggggtaat cgtgccggtg ggcacagcca gggtgtgagg tagaggcggg
      301 gtgcccgtgc ctgcagggat tacgctgccc gtgggtagag gcgggggcgt gctgaagatg
      361 gtgggcgtag tggccgctat ggtgcctgtg ggggcgggcc ggggcggggt gggcgtgccc
      421 ggcgctcctc cggggcaaag accggcgtta cggtaccgcc aatcaaagta gtgctggccg
      481 atgttgccca tctcgacctg gaagccgggg ccattggtgg gtacgtaggt gagcacgcgc
      541 cgctcgtagg cctggaccag cacgtcggtg gtctcgccgc gtatgctagc ctttgcccag
      601 tacgcttcgc ttatcggcat ccccgccgcg tagtaccacg gacttgagag ccgcccgctc
      661 gtcagctgct cgtcgctaac caggcggtcg cctctgagca ctatctcgta tacctcgccc
      721 gacgagttga gaaaatccca gaataccctg ggtatgttat ggcccgtcct ggcctcgaag
      781 taggcgatgt caacgccggg cacgctgctc ttggccgggt cgtcgcccgt cttgcccgtg
      841 ctgtcaatcg tctgcgtcac cctgtggccg gtgaggttgg gggcgtggcg gtcgccgtcc
      901 acggtgttgg agacctcttt gaaggcggcg tacgtggggg ccagcgtgtc gccaaagtcg
      961 ccggtcatgc ctatgcaagc ggggcggtag cggatgaagt cttcctctcc gacctggata
     1021 atgcccgata caagctcgcc ggcaagcagg ccgttggtga cgtaaaaatc ggtgttccag
     1081 ttgccgtggg ggtcgttgat ctccatgcgg ctcttgtcga agtactgcac cagccgctgc
     1141 ccgcggcccg aggggtcctc gttgtactgt tcgagcaagc cgggcgtgtt cggtcccgga
     1201 ccccacatcc aggtgcgggc tactttctcc tctttgatgg gcctgtcggt acgcacccag
     1261 agcagttcca tgcgcccggt gccctcgaac gggttggcga aggcgaacgg cccgccgccg
     1321 gccttgtaca ccgtctccgg cagttgcgcc gtggggatgg gggtgaccgt gcccgcgcct
     1381 gcgggtacgg ccgtaggcgt gcccgaacta cctgccgtcg gagtgccggg gggcggagtc
     1441 tgggccaatg ctacggcgct gaatagcata atcgccactc ccggtgtcgc ggcaactata
     1501 ctccatctcc tggtcatgga gctttcctcc tcgtggtcct ttgtagtcgg gtcgtgcttg
     1561 aatgctaata tcgtttgcta ggccgcagcg tgctaagccc tttggctcgg tgtcgccacg
     1621 tagataatct tacaacgggc gctactcaca acggtttcac agcttcgaat ccattgccca
     1681 aaagtggcgt aggattccac gcgccggatg catcttaaaa ctgatgcgtc ctaaacagga
     1741 tacggtgtac agtaaggtgt aggaggaggt agcacgagta gatgcaaggc ggatacactg
     1801 ccatatcaga gcgggaggca ctggtccgct tctgtgcccg gtacacggga gaccccaccg
     1861 gcgcggagga cctggcccag aagaccctgc ttgcggcctg gcagcacgag cagcagttgc
     1921 gagaccccca ggcgcgacag gcctggctct tcagcatcgc acgcagggaa tgcctggcat
     1981 gggcgcgcag ccgcgaccgc tcgcgcctgg tcaaccttga ctccctggac gaatgggaag
     2041 gccagagccg gttggccggc gacttcgaca tcgagacgga gcttgagcgc gacgacctgg
     2101 cgcgactgct cgaccgggcg atggggctgc tgccacccga cctgcgcgag gtgctcgtcc
     2161 gccggtacat cgaggaatcc tcccagggcg agctagcgga gcggctgggt attacggagg
     2221 gtgcggttga ggcgcggctg caccggggga agctcgcgct caggcgagtg ctctccacga
     2281 aattgagcga ggatgcgctg gcgtttgggc tgatctcagc ctccgatgcg ggctgggagg
     2341 agacgcgcct ctggtgcccc ggctgtggcc gccgaaggct ggaaggccgg ttccggcgcg
     2401 acgagggctg gctgtacatg cgctgcccgg actgctcccg ctccggcgac gacttcagtc
     2461 acttcatcca ctcgacgctg agcaacaaac tgagggcctt ccagagctac aagcccgccc
     2521 tctcccgcgt gctggagggc atacaccgga tgtaccgggt gcaggcccgg cgcggggcga
     2581 cacgctgccc cggttgcggc gaatggcagg cgataggcca gggcacgccg ccctgggtgc
     2641 cggagaggtt cgcggacccg cagagtgtct atctgtatca ccctgagtgt ggtgtctacg
     2701 actgggagag ctggc
//
LOCUS       DASRWZ010000623         1914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_119414, whole genome shotgun sequence.
ACCESSION   DASRWZ010000623 DASRWZ010000000
VERSION     DASRWZ010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119414"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..>1914
                     /locus_tag="VFH60_09880"
     CDS             147..>1914
                     /locus_tag="VFH60_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET6314134.1"
                     /translation="MITTKKNHTTDTTEKLRISGMDCADCAISIEKSLETVPGVRSAS
                     VSFGAGTAQVSYDPAVATHDALVRRVSELGYNVEDTIAASVQTGPLEFVIEGMDCADC
                     ARTIEKTVEALPGVAQASVNFGAARLSVTPQASAQLPETSTIERVVTAAGYRATPVAK
                     RRSGESLPFWKRERRVINTAVGTVAALTGLALSFTPAPVWLVNTFFAAGIVVAGAAFA
                     RAGLLALRAGRADMNLLMTVAAVGAVLLGDWGEAVTVVLLFAFGGTLQSYTLDKTRGA
                     IRALMDLTPATALVRRADRSNSLPLMREVRLPVEEVQPGETVLVGHGERLPLDGVVLQ
                     GTSSIDQSPITGESVPVDVEPGTEVFAGTINGPGALSIRVERAAKDTTLARIIEMVEQ
                     AQGRRAPSQQFVDRFSAIYTPVVIAAAALIAILPPLLFAQPFDEWFYRALVLLVVACP
                     CALVISTPVSIVSAIGAATRRGVLLKGGATLEALGKVTAVAFDKTGTLTEGRPRVVAV
                     RALDGDNDALLKLAGMVEARSKHPLAMSIVHAGRNLKNGASEASDFKAFPGLGARAVV
                     DGREVYVGNARLFQEQGIPVDGV"
BASE COUNT          331 a          572 c          667 g          344 t
ORIGIN      
        1 gtttggagca tgtgagggat atctgaaacg tgaaacgaac aacgtgatgg ggcttgctca
       61 tcttggttcc gtaaagtcaa ctacatagca ttcacgaacc aaataacaca agccgcatca
      121 cgtttcacgt ttcagaatca ggaacgatga taactaccaa gaagaaccac acaactgata
      181 caaccgaaaa gctgcgcatc agcgggatgg actgcgcgga ctgtgcgata agcatcgaga
      241 agtcgctgga gacagtgccg ggcgtcaggt cggccagcgt gagctttggc gcgggcacgg
      301 cgcaggtgtc gtacgatcct gccgtggcta ctcacgacgc cctggtcagg cgggtgtccg
      361 agcttggcta caacgtcgag gacaccattg cggcgagtgt gcaaaccggc cctctggagt
      421 ttgtgataga aggtatggac tgcgccgact gcgcgcgtac cattgagaag acggtggagg
      481 cgctgccggg tgtggcccag gcaagtgtga acttcggcgc agcgcggcta tcggtgacac
      541 cccaggcgtc cgcgcagctt ccagagacga gcacgattga gcgggtggtg acggcggcag
      601 gctacagggc cacacccgtt gcaaagcggc gctccggtga gagtttaccc ttctggaagc
      661 gggagcggcg ggtaatcaat accgctgtgg gcacagtcgc ggctttgact ggcctggcgc
      721 tgtcgttcac gcctgcaccc gtctggttgg tcaatacctt cttcgctgcg ggcatcgtgg
      781 tagcaggggc cgcgttcgcc agggcggggc tgctcgcgtt gcgggccggt cgcgccgata
      841 tgaacctgct catgaccgtg gcggcggtgg gcgcggtgct cctgggcgac tggggcgagg
      901 ctgtcaccgt ggtgctgctc ttcgcgttcg gcggcacttt gcagtcgtac acgctggaca
      961 agacacgtgg ggccattcgc gccctgatgg acctcacgcc cgccaccgcc ctggtgcgcc
     1021 gggccgacag gagcaacagt ctgccgctca tgcgcgaggt gcgcctgccg gtggaggagg
     1081 tgcagccagg cgagacggtg ctggtcggac atggcgagcg cctgcccctg gacggtgtag
     1141 ttttgcaggg cacttcatcc atcgaccagt cgcctatcac cggggagagc gtgcccgtgg
     1201 acgtggagcc tggcacggag gtgttcgcgg gcaccatcaa cggtcccggc gcgctcagca
     1261 tacgcgtgga acgggccgcc aaagacacca ccctggcccg catcatcgaa atggtggagc
     1321 aggcccaggg gaggcgcgcc ccttcgcagc agttcgtgga ccgcttctcg gccatataca
     1381 cgcccgtggt aatagcggcc gccgcattga tcgccatatt gccgcccctg ttgttcgcgc
     1441 agccgtttga cgagtggttc taccgggcgc tggtgctgct tgtggtggcc tgcccctgcg
     1501 cgttggtcat atccaccccg gtcagcatcg tgtcggccat aggggctgcc acgcggcggg
     1561 gcgtgctgct caagggtggg gccacgctgg aggcgctggg caaggtgacg gcggtggcct
     1621 tcgacaagac gggcaccctg accgagggcc gcccccgcgt ggtggccgtg cgcgccctcg
     1681 acggcgataa tgacgccctg ctcaagctgg ccggcatggt ggaagcacgc tccaagcacc
     1741 cccttgctat gtctatcgtg cacgcgggcc gcaacctgaa gaacggtgcc tcagaggcct
     1801 ccgatttcaa ggcgttcccc ggcctgggcg cgagggccgt ggttgatggg cgtgaggtct
     1861 acgtgggcaa cgctcggctc ttccaggagc agggcatccc ggtggatggt gtgg
//
LOCUS       DASRWZ010000624         3029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1284819, whole genome shotgun sequence.
ACCESSION   DASRWZ010000624 DASRWZ010000000
VERSION     DASRWZ010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284819"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFH60_09885"
     CDS             <1..219
                     /locus_tag="VFH60_09885"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET6314135.1"
                     /translation="GVLLGTSRKSFIGRVLAHASGGELPPPDERVIGTGATTAIGIAG
                     GVEIVRVHDVAHNVQVARLADAIVRGIK"
     gene            324..740
                     /gene="folK"
                     /locus_tag="VFH60_09890"
     CDS             324..740
                     /gene="folK"
                     /locus_tag="VFH60_09890"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET6314136.1"
                     /translation="MATSDLYCSKPWGKLDQPDFLNMVASIETEMEPRELLRKCKRLE
                     REAGRTAGERWGPRVLDIDILLYDDLTLDSDTLTVPHPRLWQRQFVLMPLAELLPELH
                     DGHGRSILEVSQSEAIQTQGVWPCGERDSKGVDEDG"
     gene            733..2517
                     /locus_tag="VFH60_09895"
     CDS             733..2517
                     /locus_tag="VFH60_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314137.1"
                     /translation="MGKYRQPAVAVIALLLAALATFVPAAAQPQQPQWATLGTLPRGT
                     WSLAPEPSDPNTMYALNSAGISRTGDKGSTWTVCSPGASMLRLVAPVRGQQGKSLLYS
                     TTATGVRVSDDGCRTWRDVPQQGITPSAAHVRWLAAYPNNMSVLYAGMDGLGGLFRST
                     DSGATWQPASKGLPPNSWVTALTADPARASTIFAGLQYTTRDHPGAYLYRSTDGGLSW
                     RSSSLGMRVLPNSGGEIAGLAWSGGSLFAATSHDGLFRSTDGGSTWGLSVMPRASGAA
                     GSRSGGLPLRVSGLYADAEGALVLGTDQGAYQSLDGGGTWVAFGPEQARGRPVVIALD
                     PGSGRVVLSSGGSAYGITLPAGRVNIAPRASATPTQAPPTPPPPAALATYTPTSTPVP
                     PTATATHTPSPTPTIALVDGPKPSDPVPPGDPSVSDYFEETRHNVKYGFRDFWRSNGG
                     LARYGFPLTEEFAENGVSVQYFERARFEFRDGKVGLGNLGTELIQGLSGPQFRALPFF
                     VSTDDRIYFGPTKHSISGPFLDFWRANGRIDGMGYPVSESYSPSDGLEIQWFERARFE
                     WHRDLPASRRIVLANIGTELLQKRGWLR"
     gene            2594..>3029
                     /locus_tag="VFH60_09900"
     CDS             2594..>3029
                     /locus_tag="VFH60_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314138.1"
                     /translation="MYILDVRLPVQLLQELVRLFQGDSTVLALGGGLSQLDVHLLENV
                     TLDTEPGRPRYGVAEIPLTEKNVSALVSSILPRVGVASRIHEVTIARGEGVDPKLLFY
                     AVDNFSHWKCIVGRYNKGAGLTARVDTKLLESLVERQIIRKYL"
BASE COUNT          544 a          969 c          979 g          537 t
ORIGIN      
        1 ggggtcctgt tgggcacctc gcgcaagagc ttcatcggtc gggtgctggc tcacgcttct
       61 ggtggagagt tacctccccc ggacgagcgc gtgataggca ccggggcaac aacggccatc
      121 ggcatagccg gcggagtgga aatcgtgcgc gtccatgacg tagcccacaa cgtgcaggtg
      181 gcccggttgg ccgatgcaat agtccgtggg attaagtagg cgtggtaatt gcctacctcg
      241 gcctcggctc gaacctgggc gaccgcgagg agtaccttcg agccgctgtc gaggccctcg
      301 gctagctgag gggcacgcga gtaattgcga cttccgacct gtattgcagt aagccatggg
      361 gcaaactcga ccagcctgat ttcctgaaca tggtcgcctc catcgagacc gagatggaac
      421 cgcgcgagtt actgaggaag tgcaagcgcc tcgaacggga ggcggggcgt acggcgggcg
      481 agcggtgggg gccacgcgtg ctcgacatcg atatactgct gtacgacgac ctcaccctcg
      541 atagcgacac actgaccgta ccgcacccgc ggctgtggca gagacagttc gtgctgatgc
      601 ccctggccga gctactgccc gagttgcacg atgggcacgg caggtctatt cttgaggtgt
      661 cgcagagtga agcaatacag acgcagggcg tatggccgtg cggcgaacgc gatagcaagg
      721 gcgtagatga ggatgggtaa gtaccgacaa ccggcggtgg ctgtaatcgc gctgttgctc
      781 gcggcgctgg ctacgtttgt gcctgccgcc gcgcagccac aacagcccca gtgggcgacg
      841 ctgggcacgc ttccgcgcgg cacctggagc ctggcccctg aaccttccga ccccaataca
      901 atgtacgcgc tcaacagcgc cggtatatcg cgtaccggcg acaaggggtc tacgtggacg
      961 gtatgttcgc ctggcgcgtc tatgttgagg ttggtagccc cggtgagggg gcagcaaggc
     1021 aagtcgctgc tctattcgac tacggctacc ggcgtgcggg tcagcgacga cggctgccgc
     1081 acctggcgcg acgtgccgca gcaaggcatc accccctccg ccgcacacgt gcgctggctc
     1141 gcggcctacc ccaacaatat gagcgtgctc tacgcaggca tggatgggct gggcggactc
     1201 ttccggtcca ccgactcagg tgccacctgg cagcccgcct cgaaaggtct cccgcccaac
     1261 tcctgggtga ccgccctcac agccgacccc gccagagctt ccaccatatt cgcggggttg
     1321 caatatacca cgcgcgatca ccccggcgca tacctctaca ggtccaccga cgggggcctc
     1381 agttggcgtt cgtcttcgct ggggatgcgc gtactgccga acagcggcgg agagattgcg
     1441 gggcttgcct ggagcggggg cagcctcttc gcggccacgt cgcacgacgg cctcttcagg
     1501 tccaccgacg ggggcagcac ctgggggctg tcggtcatgc cccgcgcctc aggggcggca
     1561 ggctcgcgga gcggcgggct gccgctaagg gtgtcgggcc tgtatgccga tgccgagggc
     1621 gccctggtgc ttggcaccga tcaaggcgca taccagtcgc tcgacggcgg tggcacctgg
     1681 gtagcgtttg ggccggagca ggcgcgcggc aggccggtag taattgccct ggaccccggc
     1741 agcgggcggg tagtgttgtc gtcggggggc agcgcatacg gcattacgct gcctgccggc
     1801 cgcgtaaaca tagcaccacg ggcttccgcc acgcccacgc aggcaccgcc tacccctccg
     1861 ccgcccgccg cgctcgccac ttacacgcct acatccacgc cagtgccccc gaccgccacg
     1921 gccacgcaca caccttcacc gaccccgact atcgcgctgg tcgacgggcc taagccctcc
     1981 gaccccgtgc cgccgggcga cccctcggtg tccgactact tcgaggagac ccggcacaac
     2041 gtcaagtatg gcttccgcga tttctggcgc tctaacggtg gcctggcacg ctacggcttc
     2101 ccgcttacgg aggagttcgc agagaacggg gtgagcgtgc agtacttcga gcgcgcccgc
     2161 ttcgagttcc gcgatggcaa ggtgggcctg ggcaacctcg gcacggagct aatacaaggg
     2221 ctgtcggggc cgcagttccg cgccctaccc ttcttcgtca gcaccgacga ccgtatttac
     2281 ttcggcccca ccaagcactc catcagtggc cccttcctcg acttctggcg cgccaacggc
     2341 cgtatcgacg gcatgggcta cccggtcagc gagtcctaca gccccagcga cggtctcgaa
     2401 atccagtggt tcgaacgcgc ccgcttcgag tggcaccggg accttcctgc gagccgccgc
     2461 atagtgctgg caaacatcgg cacggagttg ctgcaaaaga gagggtggct gaggtagtta
     2521 ccaacggcga agtcgtaggc gtatggcttg ccccggtacc gctgccgcct accaccttcc
     2581 accgggaggt cccgtgtata tccttgatgt acgtctgccg gtgcaacttc tccaggagct
     2641 agtccggctg ttccagggcg attctacggt acttgccctg ggtggcggtc tctcccaact
     2701 ggatgtgcat ttgcttgaga atgtcaccct ggacacggag ccgggccggc ccaggtatgg
     2761 tgtcgcagag attccgctaa ctgaaaagaa tgtttcagcc cttgtaagca gtatattgcc
     2821 acgcgtcgga gttgcgagca ggatccatga agtgactata gcaagagggg aaggggtgga
     2881 ccctaaactc ctgttctatg cagttgacaa tttcagccat tggaagtgta tagtcgggcg
     2941 ctataacaag ggcgctggcc tgacggctcg tgtcgacaca aagctgctgg aaagcctggt
     3001 cgaaaggcag atcataagga agtacctgg
//
LOCUS       DASRWZ010000625         4695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1352331, whole genome shotgun sequence.
ACCESSION   DASRWZ010000625 DASRWZ010000000
VERSION     DASRWZ010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352331"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="VFH60_09905"
     CDS             <1..1412
                     /locus_tag="VFH60_09905"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HET6314139.1"
                     /translation="GARSLPDRLAVVEETFAEHRINLDAPYLNPGSEDDYMPLRDLSA
                     PTPHAPILQTDRDILLLTARQIGEGICTEAIWHEGRCNLIGAQVAAPSGAGNHPRLVY
                     RTLGPDLYDGTAGVALFLAHLYAHTGDAQVYRTALGAARHALSCLARPSPQLGQGAYT
                     GWPGTLLAAMRVGALLDEPELLEEVARASRNLQPDYGKGHDLDLFSGSAGAIVALLAL
                     RDMLPGGHTLEGVTRLGDDLIVLAEVRADGFSWPSRYSKGSRNLTGFSHGAAGIGYAL
                     LKLFHATGEVRYRHAAEMAFRYERYWFDATESNWPDFREVPGRGQIRKRVQAPCSLLW
                     CHGAPGIALSRLYAYQLLGDDIYREEAVVALSTTEAATRDMLKSSTANFSLCHGLAGN
                     AEIVDIGQAALDGSMAGQGELSLEVAANGVEMYARRDCQWPCGVKGGVTPGLMLGLAG
                     IGLFYLRLHDPAIPSVLML"
     gene            1432..1947
                     /locus_tag="VFH60_09910"
     CDS             1432..1947
                     /locus_tag="VFH60_09910"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HET6314140.1"
                     /translation="MAAGNDTDGHPEDTVPTQQPLRFGHACATRPSEWPPDAHLVLAM
                     RRPRWRTRPVLEAGGRSVPAAGPSDALLNAYLGKPPRGAHNTLGLTPEMPKLTWEEFA
                     EGYRQEMRTPEGLRVLDELIDIAQQDPARPLIVLCWERLDGPSAHAHCHRTVLVELLE
                     ERARERGLATI"
     gene            complement(2031..3050)
                     /locus_tag="VFH60_09915"
     CDS             complement(2031..3050)
                     /locus_tag="VFH60_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET6314141.1"
                     /translation="MLPPLSMMAAGLALLVLTAYVLLGGADFGGGVWDFFARGKRAEE
                     QREAIATAMGPVWEANHVWLIFLIVILFSAFPVAFAALSEAFFIPFHLLLAGITLRGA
                     AFVFRAHGSDEAGPQRAWARAFGSASIITPVLLGMCLGAVSAGGIRVVDGQVRVDYFT
                     AWLTPLSIACGLLALALFAYLAATYLCVETEGALQEDFRQRTLISGGVVAALAVILLP
                     LIALQAPYLAGQLLSITALPVMAVGTALALVSLWAVLTRRFRLARITAAGQVTMLLWG
                     WALAQWPYIIYPDLTVEAAAAPDSTLTFLLATAPLGALLLIPSLFLLFAVFKGRNPEG
                     NEAGN"
     gene            complement(3034..4380)
                     /locus_tag="VFH60_09920"
     CDS             complement(3034..4380)
                     /locus_tag="VFH60_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET6314142.1"
                     /translation="MDNLSAARAQMAISLGFHMVFAALGIGLPLLMLISEGLWLRRKE
                     RVYLDLAKKWAKATGLLFAIGAVSGTALSFELGLLWPPFMKFAGPIIGPAFALEGYAF
                     FIEAIFLGLYLYGWKRLSPRAHWLCGVPVAASGMMSGVLVMSANSWMQNPVGFQIENG
                     MPINIDPIQALFNPSWGILAVHLTLASYMASAFMVAAVYAWGMLRGRRDRYHNAGLTI
                     AMVVGTITAIAQPVSGDFAARLVAETQPAKLAAMESQFTTERAAPLRIGGIPDVDNRT
                     VSFAIEIPGGLSFLATHDFNEEVKGLDAFPREDWPDVALVHIAFQVMVGSGLFILLLC
                     AIYWLLRWRKREAGKWLLRGLVLSGVLGVAATEAGWIVTEVGRQPYIIYNIMRTADAV
                     TPAPGLWVTFVSFVALYVLLAVTLIWLLLRLATGRPVEDEDHDGKQGKEASDAASA"
     gene            complement(4506..>4695)
                     /locus_tag="VFH60_09925"
     CDS             complement(4506..>4695)
                     /locus_tag="VFH60_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314143.1"
                     /translation="QGWHHIQLDVMGGVSNRGVQWLWTRPDGVREVVPPYRFRYTPTA
                     GPGTSFTWPAKPEPIICP"
BASE COUNT          921 a         1528 c         1444 g          802 t
ORIGIN      
        1 gtggcgcgcg ctcgctgccc gaccgcctgg ccgtagtaga agagaccttt gccgagcata
       61 gaatcaatct ggacgcgccc tatctcaacc caggctcaga agatgactat atgccgctac
      121 gggatttatc tgctcccaca ccacacgcac caattctcca aactgacagg gatattctct
      181 tgctcactgc gcggcagatt ggggagggca tctgcactga ggcgatatgg cacgagggga
      241 gatgcaactt gattggggcg caggtggccg ctccctctgg cgctggtaac cacccgcgcc
      301 tggtctatcg cacgttgggg cctgatctat acgatggcac ggcaggcgtt gccctcttcc
      361 ttgcccatct ctacgctcat accggggatg cccaggtcta ccggacggcc ctgggcgctg
      421 cccgccacgc gctctcttgc ctcgccagac cttcacccca gttggggcaa ggagcttaca
      481 ccggctggcc ggggacgctc cttgccgcca tgcgcgtcgg ggcgctgctt gacgagccgg
      541 aactgcttga agaggtggcg cgagcctcgc gaaacctaca gcctgactat ggcaaaggcc
      601 acgacctgga cctcttctca ggaagcgcgg gggcaatcgt tgccctgctg gcattgcgag
      661 atatgctgcc tggcggacat accctcgaag gcgtcacccg cctcggcgac gacttgattg
      721 tgctagccga ggttagagcg gacggtttct cgtggccttc ccgctactct aaaggcagcc
      781 gtaatctcac gggcttctca cacggggcgg cgggcatcgg ttatgccttg ctcaagttgt
      841 ttcacgccac cggagaggtg aggtaccggc atgccgccga aatggctttc cggtatgaac
      901 gctactggtt cgacgccacc gagagtaatt ggcccgattt ccgcgaagtg ccgggccggg
      961 gccagatcag aaagcgtgtg caagcgccct gctccctgtt atggtgccat ggcgcacccg
     1021 gcatagccct atctcgcctc tacgcgtacc aactgctcgg tgacgatata tacagggaag
     1081 aagccgtggt agctctctcg accaccgagg cggccacccg cgatatgctc aagtcgagca
     1141 cggcgaactt ctctttatgt catggcctcg cgggtaatgc cgagatagtg gacatagggc
     1201 aagctgccct ggacgggagc atggctgggc aaggcgaatt atcgctggaa gtagccgcca
     1261 acggcgtcga gatgtacgca cgccgggact gccagtggcc ttgcggggtg aagggtggag
     1321 tgaccccagg actcatgctc ggcctcgcgg ggattggcct cttctacctc cgcctgcacg
     1381 accctgcaat cccctcggtg ctgatgctct aatgtatagg agaggacaac gatggcagca
     1441 ggaaacgaca cagacggcca cccggaagat actgtgccaa cgcaacaacc cctacgcttt
     1501 gggcatgcgt gcgctacgcg cccctcggaa tggcctcccg acgcgcacct ggtgctcgcc
     1561 atgcgccgcc cacgctggcg gacccggccc gtgcttgagg caggcggcag gagcgtgccc
     1621 gccgccggac cttcggacgc tctgctgaac gcatatttgg gcaaaccccc tcgcggcgct
     1681 cataacaccc tcggcctcac gcccgaaatg ccgaagctaa cctgggagga gttcgcggag
     1741 ggctatcgcc aggagatgcg aacgccggag gggttgcgcg tgctagatga gctgatagat
     1801 attgcccagc aggaccctgc gcgccctctg atagtgcttt gctgggaacg gctcgacggt
     1861 cctagtgccc acgcgcactg ccaccgcacc gtcctcgtgg agttgctcga agagcgggca
     1921 cgcgagcggg ggctggcgac gatatagggg ccaggggtca ggtgatgggt gatgggtgtt
     1981 gggatttact gcttctatgg ggcatttgat aactttacct tgcaggcggg ctaattacca
     2041 gcttcgttac cttcagggtt gcggcctttg aagacggcga agagcaggaa cagcgagggg
     2101 ataaggagaa gggcacccaa cggcgcggtc gccagcaaga aggtgagggt cgagtcgggt
     2161 gcggctgcgg cctcgacggt caggtcgggg tagataatgt agggccattg ggcgagcgcc
     2221 cagccccaca gcagcatagt cacctggccc gccgctgtta tgcgcgccag ccggaagcgc
     2281 cgggtgagca ccgcccacag tgacaccagc gccagcgccg tgccaaccgc catcaccggc
     2341 agcgccgtta tgctgagcaa ctgcccggcc aggtacgggg cctgtagagc tatcaacggc
     2401 agcaggataa ccgccagcgc cgccactacc ccgcctgaga tgagcgtgcg ctgcctgaag
     2461 tcctcttgca gcgcgccctc cgtctcgacg cagaggtacg tcgccgcaag gtaggcgaac
     2521 agggccaggg cgagcaggcc gcacgctatg gataggggcg tgagccatgc cgtgaagtag
     2581 tcaacccgta cctgcccgtc caccacccgt attccgcccg ccgataccgc gcccaggcac
     2641 atcccaagta acaccggcgt gatgatgctc gcgctgccga aggctctcgc ccaggcacgc
     2701 tgcggccccg cttcgtccga accgtgcgcc ctgaagacga acgccgcgcc ccgcagggtt
     2761 atgcccgcca gcagaaggtg gaacggtatg aagaaggctt cgctcagcgc cgcgaacgct
     2821 accggaaacg ccgaaaagag tatcacgatg aggaagatga gccatacgtg gttagcctcc
     2881 cacactggac ccatagccgt agcaatcgcc tcgcgctgct cctccgcccg cttgccccgc
     2941 gcgaagaagt cccacactcc gcccccgaag tcggccccac ccagcaggac gtaggccgtc
     3001 aacaccagca gcgcgagccc ggcggccatc atgctaagcg gaggcagcat cactcgcctc
     3061 cttgccctgc ttgccgtcgt ggtcctcatc ctctacaggc cgcccggtcg ccagcctcaa
     3121 gagcaaccat atcagcgtaa cggccagcag cacgtacagc gcgacaaagc tcacaaacgt
     3181 cacccataag ccgggcgcgg gggtcactgc atctgctgta cgcatgatgt tatagatgat
     3241 gtagggctgg cggcccactt cggtcactat ccaccccgcc tcagtcgccg ccacacccag
     3301 cacaccactc agcactaaac ctctgagcag ccatttgccc gcctcgcgct tgcgccaccg
     3361 caacagccag tatatagcgc acagcagcag gatgaatagg ccgctaccca ccatcacctg
     3421 gaaggcgata tgcaccagcg ccacgtcggg ccagtcctct cttgggaaag cgtcgaggcc
     3481 ctttacctcc tcgttgaagt catgcgtagc gaggaaactg aggccgcccg gaatctcgat
     3541 tgcgaaggac accgtccggt tgtccacgtc gggtatgccg ccgatgcgca ggggcgcggc
     3601 ccgctcggtg gtgaactgcg attccatcgc ggccaacttt gcaggctggg tctcggccac
     3661 caatcgggcc gcgaaatctc cactcacagg ctgggctatg gccgttatcg tgccgaccac
     3721 catcgcgata gtgaggcccg cgttgtggta gcggtcgcgc ctcccccgca gcatgcccca
     3781 ggcgtaaacc gccgccacca tgaaggccga cgccatgtac gatgccagcg tcaggtgtac
     3841 cgccagtatg ccccacgagg ggttgaacag cgcctgtatg gggtctatgt ttatgggcat
     3901 gccgttctcg atttggaagc ccaccggatt ttgcatccag gagttagcgg acatcaccag
     3961 tacgcccgac atcataccgc tcgcggcaac aggtacgccg cacagccagt gggcgcgcgg
     4021 gctgaggcgc ttccagccgt agaggtagag gcccaggaag atcgcttcga taaagaaggc
     4081 gtacccttcg agggcgaaag ccgggccgat gatggggcct gcgaacttca taaagggcgg
     4141 ccagagcagg ccaagctcga aggagagggc cgtgccgctc accgcgccga ttgcgaaaag
     4201 cagccccgtg gccttagccc acttcttcgc gaggtcgagg tacacccgct ccttgcgccg
     4261 cagccacagc ccctccgaga tgagcatgag cagaggcagg ccgatgccaa gcgccgcgaa
     4321 gaccatgtgg aaccccaacg aaatcgccat ctgagcgcgt gctgctgaca ggttatccat
     4381 acgtaaaccc ctacgcagta ccgagtaccg agtaccgagt accgagtgcc gagtgccgag
     4441 tgccgagtgc cgagtgctga gtgttacttc tgccttcttc tggtcactga ccactgacca
     4501 ctacatcacg ggcaaatgat aggctccggc ttggcgggcc aggtgaagga cgtgcctggc
     4561 ccggcggtgg gagtgtagcg gaagcggtac gggggcacta cctcgcgcac gccgtccggg
     4621 cgcgtccaaa gccattgcac tccccggttg ctgaccccac ccatgacatc cagttgtatg
     4681 tggtgccacc cctga
//
LOCUS       DASRWZ010000626         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_462168, whole genome shotgun sequence.
ACCESSION   DASRWZ010000626 DASRWZ010000000
VERSION     DASRWZ010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462168"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VFH60_09930"
     CDS             complement(<1..539)
                     /locus_tag="VFH60_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET6314144.1"
                     /translation="MEWLLNPDSLIALATLTLLEIVLGIDNIIFISILAGRLPKEQQG
                     TARTIGLGLAMFTRILLLLSLSWIISLTAPLFALFGQEFSGRDLILLGGGLFLLAKAT
                     WEIHHRLEGEPDGHAAQNSSRARSAFVSTLVQIILLDIVFSLDSVITAVGMANELWVM
                     ITAVVIAVGIMMVSAGGISR"
     gene            803..2233
                     /locus_tag="VFH60_09935"
     CDS             803..2233
                     /locus_tag="VFH60_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6314145.1"
                     /translation="MAKGATYDAVIVGSGPNGLAAAITMSRAGKSVLLIEAEETLGGG
                     TRSSELTLPGFTHDVCSAIHPLAVASPFFRGLPLEEHGLEWVHPPVPLAHPLDDGSAA
                     LLERSIEATGESLGPDAKAYRRLVGPIVRDWSRLEASVLGPLRFPRHPVALVRFGLRA
                     LLPARLLAHTAFRGEHARALFAGMAAHSMLSLDQPLSAASGLLLGALGHVAGWPMTRG
                     GSQRLADALVSYLRSLGGEVVTGWRVKSLDELPSARAVLLDVTPRQLLAIAGHKLTHG
                     YRSQLRHYRYGPGVFKLDLALDGPIPWAAPECSRAGTVHVGGTLDEITDSEGAVKRGR
                     HPRKPFVLVAQQSLFDPTRAPGGKHTVWAYCHVPNGSTVDMTARIEAQIERFAPGFRE
                     RILARHEMSPTDVHRHNANYIGGAIGGGVQDLRQLFTRPAVRLVPYTTPARDVYICSS
                     STPPGGGVHGLCGWEAARVALRRSFK"
     gene            2717..>3261
                     /locus_tag="VFH60_09940"
     CDS             2717..>3261
                     /locus_tag="VFH60_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314146.1"
                     /translation="MPGVTADRSQGQTEAVSPPLLSRRWRRSAHVLLVALLLLTWGWL
                     TWQVVIPAMRANVWPYSAYWIGPKFALEGRVHLFYADSSVYLPEIARLNVTADLLEVN
                     APTTLLLFLPLGLLSIGEAFRAWTVISLACFAVGWWGLLRAVRLPLWVGLLLTSAVPL
                     FEPFVANVRGQSYHVLFSLAAL"
BASE COUNT          573 a         1023 c         1042 g          623 t
ORIGIN      
        1 cggctgatgc ccccggccga taccatcatg atgcccaccg cgatcactac ggcggtaatc
       61 atcacccata gctcattcgc catgcccacg gcggtgatca ccgaatcgag ggagaagact
      121 atatcgagca ggattatctg cacgagagtg gagacgaaag cgctccgtgc ccgcgagctg
      181 ttctgggccg cgtgaccgtc cggctcgccc tccagcctgt gatgtatctc ccaggtggct
      241 tttgcgagca ggaacagccc tccacctagc aggatgaggt cgcggcccga aaactcctgg
      301 ccgaacaggg caaagagggg cgcggtgagg ctaatgatcc acgaaagcga gagcagcagc
      361 aatatacgcg tgaacatagc cagccccagg ccgatcgttc gagcagtccc ttgctgctct
      421 ttcggcaatc ggcccgccag gatcgagatg aagatgatat tgtctatgcc taacactatc
      481 tccagcagcg taagggtcgc cagagctatg aggctgtcgg ggttcagtag ccattccatt
      541 aacctgtcct ccaggagtga ttatttgcaa gtcgcgctac acctggtgta tagctcgacg
      601 ttaacgcgga caccctccat ggggcgcgtg cggcagcttt gccaggcgtg tgcctcgtgc
      661 agatagcatg gcctggcatg tgggcggaaa agccgggtac gtcctgatcg ggatgaggca
      721 atatccctgc caccatgtac aatatcagcg tatcaccgtg gcacactcgt aagaagtgag
      781 ggagtagcga caaggagagg gcatggcaaa gggggcgaca tacgatgcgg ttatagtggg
      841 gtcgggtccc aacggcctgg cagccgctat tacgatgtcg cgagcgggca agtcggtcct
      901 gctaatcgag gcggaagaga cgcttggtgg gggcacccgc tcgtccgagc tgacgcttcc
      961 aggcttcacg cacgacgttt gctcggccat acacccgctg gcggttgctt cccccttctt
     1021 ccgagggctg ccgctggagg agcacgggtt ggagtgggta cacccacctg tcccgctggc
     1081 acaccccctc gatgacggct cggcagccct gctcgaacgc tctattgagg ccacgggcga
     1141 gtcgctcggc cccgacgcca aagcgtacag gcggctggtg gggccaatcg tccgcgactg
     1201 gagcaggctg gaggcgagcg tcctggggcc gctcaggttc cccaggcacc ccgtggccct
     1261 ggtgcgcttc ggcttgcggg cgctcctccc agcgcgtctg ctggcccaca cggcgttccg
     1321 gggagagcac gcccgcgcgc tgttcgcagg catggcggcc cactcgatgc tgtcgctgga
     1381 ccagccgttg agcgcggcgt ccggcctgtt gctgggggcg ctggggcacg tcgcgggctg
     1441 gcccatgacg cgtggcggct cgcaacggct ggccgatgcg ttggtgtcgt acttgcgctc
     1501 cctgggcggg gaggtagtca caggttggcg ggtgaagtcg ctggacgagt tgccttcagc
     1561 cagggccgtg ctgctggacg taaccccgcg ccaactgctg gccatcgcgg gccataagct
     1621 tacacacggt taccgcagcc aactacggca ctaccgctac ggccccggcg tgttcaagct
     1681 cgacctggcc ctggacggcc cgataccctg ggcggccccg gaatgctcgc gggcgggcac
     1741 tgtccatgtg gggggcacgc tggacgagat aaccgactct gagggggccg taaagagagg
     1801 caggcaccca cgcaagccgt tcgtgctcgt ggcccagcag agcctgttcg acccgacccg
     1861 cgcgcctggg ggcaagcaca cggtgtgggc ctactgccac gtcccgaacg gctccaccgt
     1921 ggacatgacc gcgcgcatcg aggcgcagat agagcggttc gcccccggct tccgcgagcg
     1981 catcctggcc cggcacgaga tgtcgcccac cgacgtgcac aggcacaacg ccaactacat
     2041 cggcggcgct atcggcggcg gcgtgcagga cctccggcaa cttttcaccc gccccgccgt
     2101 gcgcctagtg ccctacacca cccccgcccg cgacgtctat atctgctcgt cgtccacccc
     2161 gcccggcggg ggcgtgcacg gcctgtgcgg ctgggaggcc gcgcgggttg cgttacggcg
     2221 gagcttcaag tagacaatag acgatagcat agtgacctgc tgcgccgcat ggacgccttg
     2281 cgatagtcct gtggctgaag ccacgggctg ggaagtagcg aagtctcctt cggagactag
     2341 gtggtatctg caaattgcaa gtagtgcggt acctagttcc cctctgtcat tctgagcgta
     2401 gcgaagaatc tcgtccctca gcacccaaat accacttttc ttcacgccag caaggtacca
     2461 tgtgcctgca tgtgctgagg aaaagggaag catcagggta gaggacgaga ttcttcgctg
     2521 cgctcagaat gacaagggcc acctctgttc gcttacttcc tacactttgc agatacctcc
     2581 taccatcatg taagagagtc cccgtagggg actttgctac gtctcagccc gtggcgtcag
     2641 ccacagggcg tccccacagg gcaatcctgc cacgacaccc ggccatattg cagagctgta
     2701 ttatcattag gttgtcatgc caggtgttac cgccgacaga agccaggggc aaaccgaagc
     2761 cgtgtcgccg ccgttgctat cgagacggtg gcgccggtcc gcgcacgtct tgctggtggc
     2821 attgctgctt ctcacctggg gttggctaac ctggcaggtt gttataccgg cgatgcgggc
     2881 gaacgtctgg ccctactcgg cgtactggat cggaccgaag tttgcgcttg aagggcgcgt
     2941 ccacctgttc tacgccgata gctcggtgta cctgcctgag atagcgcgac tgaacgtaac
     3001 cgccgacctc ctggaagtca acgcgcccac taccctgctg ctcttcctgc cgctcggctt
     3061 gctctcgata ggcgaggctt tccgtgcatg gactgtgatc agtctcgcct gctttgcggt
     3121 aggttggtgg ggccttttgc gggcggtccg gttgccgctt tgggtggggt tgttgctcac
     3181 gtctgcggtg ccgcttttcg agccgttcgt cgcgaacgtc aggggccaat cgtaccacgt
     3241 gctgttttcg ctggcggcgc t
//
LOCUS       DASRWZ010000627         7825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_736476, whole genome shotgun sequence.
ACCESSION   DASRWZ010000627 DASRWZ010000000
VERSION     DASRWZ010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736476"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="VFH60_09945"
     CDS             complement(<1..1767)
                     /locus_tag="VFH60_09945"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6314147.1"
                     /translation="MQRAQLAIVPDAQPDAGAAVRHNLPAQPTRLIGRHSELATVLDL
                     LRSPDARLLTLTGPGGVGKTRLATEVATRLVDTFPDGVCMVELAALTTPPTVVTTIAG
                     ALNLRETAGVPLVTTLADYLYDKQFLLLLDNFEQVLPAAPVVAELLASCPSLKLLVTS
                     REVLRLRGEHEVALSPLPLPHIEGSAPPNVEALAQNEAVALFMQRAVAVRPNFQLDDS
                     NARAIAEICIRLDGLPLAIELATARLKLLSPQALLARLRQALKILTGGANDLPARQQT
                     LRDTIDWSYNLLDAYEQCFFRLLAVFSGGCNLEAVEAVASGPGSDEGLLALERVSSLV
                     DKSLLRRLDQPDGEPRLLMLETIRDYAMERLIESGEDEAAHERHAAYFATFAEAAEKE
                     LRGPDQARWLDMLEADRGNLRAALAWSTHNETATSYERVEYGLRIAGALAQFWQIYGP
                     LSEGREWLEAVLKVPEGQAITSARAKAQFSAGWLALFQGEYEVAVSHCAESLTINRQL
                     GDDWNATWALNGLGRAMEATGDLDRATAYYEEGLAIRRTMGNQWGVGGSLNNLGRVAM
                     RQGDYDRAAALFEEGLSVFREVG"
     gene            complement(2013..4244)
                     /locus_tag="VFH60_09950"
     CDS             complement(2013..4244)
                     /locus_tag="VFH60_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314148.1"
                     /translation="MAKAARRNSAANTWARAGVAGVLVMLALGLASGGGSSQAAAPKQ
                     PILPASPQAANQNYGFFMALLGNNTEVRNMGFQWVQYGVYWSTSEPTEGNYQWGDVEN
                     IVNNARDAGLNVLIRISRTPEWARDSRCSGVDRCPPADPADFGRFSAALAAKVRSLTP
                     NAVAYEIWNEPNTNDEWGGNDPDMCPEPEKYANLLKAAYPAIKNADSTALVLGGAVTT
                     VGERLRPNVCALDDITFLERMYDAGAKPYFDILANHPYGFAAAPEAPPVGGENRLTFR
                     RAERHRDVMLQYGDGAKKVWATEMGWAVDPHLEGKCTNKTFPWYFQFTPEQQSDYLVR
                     AFQWSRSYWPWMTGMFVFNFDFNEAPWYQECDDFNFMSVKGRPAQTALSAFVQNPPAT
                     YTPVPATATPVLDLPPAITAVRYSATEFNRFGGTLTVEVDANDPDSSPIDVVQANLSF
                     PDGAYQVFTFELVAGTAEQGTWRTTIPIDANDTGGAQTYSLAPYAVETYPGRRTAAAP
                     VQNIIVHATRFIDVPEGYWAYEYIEGLAERGAISGYGDNSFRPGNSTTRAQLTKITML
                     GFGYPLVEDAQQTFADVPPGSPFHTYVETAARRSIIGGYPCGRTAEPCDEQSRPYFRP
                     NNSITRGQITKIVVLSAGWPELNERPQTFSDVQPGSPFYGFVESAADHQIIGGYPCGS
                     AGEPCDPARRPYFRPNDTTTRAQIAKMVYLALQQNAPATPTATSATSQPPTKATAVTK
                     SKP"
     gene            complement(4478..5887)
                     /locus_tag="VFH60_09955"
     CDS             complement(4478..5887)
                     /locus_tag="VFH60_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6314149.1"
                     /translation="MCVTLFAPGALPSHSRAAAQAQDAPPVPMLYGSAPDAPDSQLQA
                     IVEQTVGELGGDWGVAVKKLDTGQYAAFNGDTQQVSASLYKLWVLGELFGQARLGIVN
                     LEGTSTITPQDAAYDASLGILRISVGAVVTLREAANLMVKRSDNTTSVLLVRTLGPDN
                     IVRFMRDIGLKNSVLDWSGAGDNLTTPIDVLHVLEMIATSRMVDAESSKEMVDIMLGQ
                     QFNDLLAPGLPSGGRFAHKTGELDGLLHDAGIVYGPSGPFLLVAMSSNLPSYALARQN
                     MPILMERVYRYFNDRAYVPARYFPETRRTVGHDFLKFYNMYGGLTTFGYPIGPERVVD
                     GTLVQQFERARMEWHPELAHGAVPVPGVVLSPLGQQRANELRLTWPASAANGSERYFR
                     ETGHGIRGGFLEYWLNNGGERIFGLPISPEVSLRNPTDGKTYTTQYFQRARMELHPEL
                     PEGQRIVLGTLGKEVATGP"
     gene            6323..6586
                     /locus_tag="VFH60_09960"
     CDS             6323..6586
                     /locus_tag="VFH60_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HET6314150.1"
                     /translation="MNSSLIGKIEKAQRYEQEPERVQIQTLEADFRGEHDMYHIKLSN
                     NHWSCSCSFYSGYGTCSHVMAAQRILSPMLSLESRTESPLAHA"
     gene            complement(6672..>7825)
                     /locus_tag="VFH60_09965"
     CDS             complement(6672..>7825)
                     /locus_tag="VFH60_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314151.1"
                     /translation="GLALRKYGIPFARKLSSTTSNPDHIGAMQVAATVTYAWNPLALI
                     EFGANGHNDVLMLTFVLCALWMHLKGWWPLAVSALAAAVLVTMIAVVLVPGYLWLLLW
                     QGRTERPPWDRESWQGGILRMTQAACLFAGLVALAYLPFWEGPGMLRSITGGPPATRT
                     VNSLADILKFQGAEWVSAFAHAQPWYPYRFWEPDAIAARLDWPLRWGPLMIAAAWAIL
                     RTWGARTFPEMLRAWGWVLLVYLTVASVWYWPWYASWLLVPVALIGTGRLMRASLILT
                     ASSMAMYGMFPVMAPPFDNFPRWTGLVIMGPPLLYLLGTWLRDRVAKRRLAPVPQGAL
                     LPSHGSGLTQAAVPVPLRPEPLFAMYSEGGTAQVQHFAPDPAREDRELF"
BASE COUNT         1406 a         2417 c         2557 g         1445 t
ORIGIN      
        1 gcccacctcg cggaacacgg acagaccttc ctcgaagagc gccgcggcgc ggtcgtagtc
       61 cccctgacgc atagcgaccc ggccgaggtt gttcaaggag ccgccgacgc cccactggtt
      121 gcccatcgtg cggcgaattg ccagcccctc ctcgtagtag gcggtagcgc ggtcgagatc
      181 gcccgtggcc tccatcgccc tgccgaggcc gttgagggcc caggtggcgt tccaatcgtc
      241 gccaagctgc ctgttgatcg tgaggctctc ggcgcagtgg cttacagcta cctcgtactc
      301 tccctggaag agggcgagcc aaccggccga gaactgggcc ttggcgcggg cggacgtgat
      361 ggcctggccc tccggcactt taagcaccgc ctccaaccat tcgcgcccct cgctcaacgg
      421 cccgtatatc tgccagaact gcgccagcgc ccccgctatc cgcaggccgt actcgacccg
      481 ctcgtagctc gtcgcagtct cgttgtgggt gctccaggcc agggcggccc gcagattacc
      541 tcggtccgct tcgagcatgt cgagccagcg ggcctggtcc ggcccccgca attccttttc
      601 ggcagcctcc gcgaatgtcg cgaagtaagc ggcatgtcgc tcgtgcgcgg cctcgtcctc
      661 gcccgactcg ataaggcgct ccattgcgta gtcgcggatc gtctcaagca tgaggaggcg
      721 tggctcgccg tcgggctggt cgaggcgtcg aagcaggctt ttgtccacaa gggacgacac
      781 ccgctcaagg gccagcagcc cctcatcgga gccggggcca cttgctacgg cttccacggc
      841 ctcgaggttg cacccgccgg aaaagacggc tagcagacgg aagaaacact gctcgtaggc
      901 atccaacagg ttgtagctcc agtcgatcgt gtcgcggagc gtctgctggc gcgcggggag
      961 gtcgttagcc ccacccgtga ggatcttcag ggcctgtcgc agccgggcga gtagcgcctg
     1021 tggcgaaagc aacttcaagc gtgccgtcgc cagttctatg gccaggggca gaccgtcgag
     1081 ccttatgcag atttcggcaa tggcgcgggc gttgctgtcg tcaagctgaa agttggggcg
     1141 cacggccacg gcacgctgca tgaagagcgc cactgcctcg ttttgggcaa gggcctccac
     1201 gttgggtggc gcgctaccct cgatgtgggg gagaggcaag ggggacagcg ccacctcgtg
     1261 ctcgccgcgt aacctgagca cctcgcggct ggttacaagc agcttcagcg aggggcacga
     1321 ggccagtagc tcggccacga ccggggcggc gggtagcact tgctcgaagt tgtccaggag
     1381 gaggaggaat tgcttatcgt agaggtaatc agcgagagta gtgacgagtg gaacacccgc
     1441 ggtctcacgc aggttgaggg caccggcgat agtggtcacc accgtcggcg gtgtagtcag
     1501 cgcggctagc tccaccatac agacgccgtc ggggaatgtg tctaccagcc tagtagcgac
     1561 ttcggtcgcc agtcgagtct tgcctacgcc ccccggcccc gtcagcgtga gcaagcgcgc
     1621 gtcgggactt cgcagcaggt ccaagaccgt tgccagctcg gaatgccgcc cgatgaggcg
     1681 tgtgggttgt gcaggcaggt tgtgacggac cgctgccccc gcgtccggtt gcgcgtcggg
     1741 gacaatggca agctgggcgc gttgcataac gacctccgag cacattatag caggtagctg
     1801 gcctgtgtac actacccgcc tgcctggaat caacgcactt gccagcagat tcagcatccc
     1861 gagcgtagat cagcagttct acaccatgct catgcgccct acacatggct gcacctctcg
     1921 aatatctctg tggggtgcgg cggggaaaga aagacctcct ccggtttggc acctgagaag
     1981 gtctctattt gcggatgcgc ggcagacaag tgctacggct tactctttgt caccgccgtg
     2041 gccttcgtgg gaggctgcga cgttgcgcta gtagcggttg gggttgcagg tgcgttctgc
     2101 tgaagcgcca ggtagaccat cttcgcgatt tgggcgcgag tggtcgtatc gttagggcgg
     2161 aagtaggggc ggcgggcagg gtcgcaaggt tctcccgctg acccgcacgg gtagccgccg
     2221 atgatctggt gatccgcagc cgactccacg aatccgtaga agggagagcc gggctgtacg
     2281 tcgctaaagg tctgcggcct ctcgttcagt tcgggccagc ctgccgacag cacgacgatc
     2341 ttggttatct gcccgcgcgt gatgctgttg ttgggccgga agtagggtct gctctgctcg
     2401 tcgcacggct cggcggtcct gccgcaggga tagcccccaa tgatgctccg gcgggcggcg
     2461 gtctctacgt aggtgtggaa ggggctgccg ggcggcacgt cagcgaacgt ttgctgcgcg
     2521 tcctctacca gggggtaccc gaagcccagc attgtgatct tggtaagctg agcgcgcgtg
     2581 gtcgagttgc ccggcctgaa gctgttgtca ccgtagccgc taatcgcccc tcgttccgcg
     2641 aggccctcga tgtactcata cgcccagtag ccttccggta catcgataaa gcgcgtcgca
     2701 tgcacgatta tgttctgcac cggggccgcc gccgtccgcc tgccggggta agtctctacc
     2761 gcgtacggcg caagcgagta ggtctgggca ccacctgtgt cgttggcgtc aatggggatg
     2821 gtagtgcgcc aggtgccttg ctcggccgtg cccgcaacaa gctcgaaggt gaacacctgg
     2881 tacgcaccgt cggggaagct gaggttggcc tgcacgacat caatcgggct gctatcgggg
     2941 tcgttggcgt caacctccac cgtgagagtg cccccgaagc ggttgaactc ggtggcgctg
     3001 tagcggaccg ccgttatcgc cgggggcagg tctaagacgg gggttgcggt ggcgggcacg
     3061 ggcgtgtagg tggcgggcgg gttctggacg aaagccgaga gcgcagtttg cgcgggacgg
     3121 cccttgacgg acataaagtt gaaatcgtcg cactcctggt accagggggc ttcgttgaag
     3181 tcgaagttga agacaaacat gccggtcatc cagggccagt aggagcggga ccactggaag
     3241 gcgcgtacga ggtagtcgga ctgctgttcg ggcgtaaact ggaagtacca ggggaaggtc
     3301 ttgttggtgc acttaccttc caggtgtggg tccacagccc agcccatctc ggtcgcccat
     3361 accttcttgg caccatcgcc gtactgcagc atcacgtccc ggtgcctctc cgcacgccgg
     3421 aacgtcagtc ggttctcgcc cccaactgga ggggcctccg gggctgccgc aaagccgtaa
     3481 gggtggtttg cgaggatgtc gaaatagggc ttggcacctg cgtcatacat ccgttccagg
     3541 aaggtgatgt catcgagggc gcacacgttg ggtcgcagtc gttcgcccac cgtagtgacc
     3601 gcgccgccga ggaccagggc ggttgagtcg gcgtttttga tggccgggta cgcagccttc
     3661 aggaggttgg cgtacttttc cggctccggg cacatgtccg ggtcgttgcc gccccactcg
     3721 tcgttggtgt ttggctcgtt ccagatctcg taggccacgg cattcggcgt aaggctccgc
     3781 accttggccg ccagcgccgc gctgaagcga ccgaagtcgg cggggtctgc gggcgggcac
     3841 ctgtccacgc ctgagcacct ggagtcccgc gcccactcag gcgtccgact gatgcggata
     3901 aggacgttga ggccggcatc tcgggcgtta ttgacgatgt tttctacgtc gccccactgg
     3961 tagttgccct cggtaggctc agaagtgctc cagtagacgc cgtactgcac ccactggaag
     4021 cccatgttcc tgacctcggt gttgttgccc agtaacgcca tgaagaagcc gtagttctgg
     4081 ttggcggcct ggggcgacgc gggcaggatg ggctgcttgg gtgccgctgc ctggctgctt
     4141 ccgccccctg acgcgaggcc gagggccagc atcaccagca caccggccac tcccgctctt
     4201 gcccacgtat ttgccgctga attgcgcctt gctgcctttg ccaagttatc agtccttcct
     4261 tgcctgagtg tttgccggtg cccatactag catagttaac gcggacgtgc gtccggttct
     4321 ccacgtatcg cgtggtccct cattattaac ggggtagggt tgctcgcggt tgctagttac
     4381 cgaagcttag ggcgtaaatc ttcgtaaatc tcggagcaaa aagaaagggt gccgctagcg
     4441 cacgcaaggc acccacgttt cactcagggc ctggcggcta aggtccggtg gccacttcct
     4501 tgccgagggt gccgagcacg atgcgttggc cttccggcag ctccgggtgc aactccatgc
     4561 gggcgcgctg gaagtactgc gtcgtgtaag tcttgccatc cgtcgggttc cgcagcgaca
     4621 cttcgggcga gattggaagg ccgaatatgc gctcgccgcc gttgttcagc cagtactcga
     4681 ggaagcctcc cctgatgccg tgtcccgtct cccggaagta gcgctcgctg ccgttcgcgg
     4741 cgctcgcggg ccacgtgagt ctaagctcgt tggcgcgctg ctgcccaagc gggctgagca
     4801 ccacgccagg cacgggcacc gcaccgtgtg caagctccgg gtgccactcc atacgcgcgc
     4861 gctcgaactg ctgcaccagc gtgccatcga ccacccgctc aggcccaatc gggtagccga
     4921 acgtggtgag gccgccatac atattgtaga acttcagaaa gtcgtgcccg accgtccggc
     4981 gcgtctccgg gaagtacctg gcgggcacgt acgctctgtc gttgaagtac ctgtacaccc
     5041 gctccatcaa gataggcatg ttctggcggg cgagggcgta gctcggcagg ttgctggaca
     5101 tcgcgaccag caagaaggga ccgctagggc cgtagacgat tccggcatca tgcagcaggc
     5161 cgtccagttc tcctgtcttg tgggcgaagc ggccgcccga gggcagcccc ggcgctagga
     5221 ggtcattgaa ctgctggccg agcatgatgt ctaccatttc cttgctagac tcggcgtcca
     5281 ccatccggga ggtcgcgatc atttccagca cgtgcagcac gtcaattggg gtcgtgaggt
     5341 tgtcgcccgc tccagaccag tcgagcaccg aattcttgag gccgatgtcc cgcatgaacc
     5401 gcacgatgtt gtcgggcccc aacgtgcgca ccagcaatac cgatgtggtg ttgtcgctgc
     5461 gcttgaccat taggttggcg gcttcacgga gggtgaccac cgcgcctact gaaatgcgga
     5521 ggatgccgag ggaagcgtcg taggccgcgt cctggggggt gatggtgctg gtgccttcca
     5581 ggttgacgat gccgagcctg gcctggccga acagctcgcc gagcacccat agcttgtaga
     5641 gggacgcgga gacttgctgc gtgtcgccgt tgaaagccgc gtactgcccg gtgtccaact
     5701 tctttacggc cacgccccaa tcgccgccca actccccgac tgtctgctcc actatggcct
     5761 gcaactggct gtctggagcg tccggggcgc tgccgtagag catcggcacg gggggcgcgt
     5821 cctgtgcctg cgccgccgcg cgcgagtggg agggtagggc accgggggcg aataaggtga
     5881 cgcacataac cacgcacagg agggtggcgg cacgcttgag agtaacgcgt ctggacgcga
     5941 aggactgtag tagcactcca atcttcatgt gcgaattata tcacaaaccg gccatcggaa
     6001 aagcgaaggc cggcaaagta gtaggccgta accgggacta atttctgccg gaatctcacc
     6061 gccgaaatgg tggatatcgg gttgacttaa gcaggggacg gggctataat ggggcaaatc
     6121 tccgaacaac ggagggctat cgttatgaga atactagctt caggggtacg tggtctgata
     6181 aggctgctac cctgtgcgtg cggataaaaa caccggctgc aagtgcgtct tgcgccggtg
     6241 tttgtgttta gcggcttatg gcagcggagg gattgctgcc ctggtataag cggacggtca
     6301 ttctttacca acggaggtca caatgaattc gagcttgatc ggaaagatag agaaggcgca
     6361 gcgctacgaa caggaaccgg agcgggtcca gatccagact ttggaagccg atttccgggg
     6421 cgagcacgac atgtatcata tcaagctttc caacaatcat tggagctgct catgcagctt
     6481 ctactccggc tacggaacgt gcagccacgt catggcggcg caacgcattc tctccccgat
     6541 gctttcactc gaaagccgca ccgaatctcc gctggcccac gcctagctcg cggcggcttg
     6601 ccgtgacatc gaacaatcga acacatgaaa gagggccgca ccgtgcggcc ctcttttgct
     6661 tgcggtttcc cctagaacaa ttctcggtct tcgcgggccg ggtccggcgc gaaatgttgg
     6721 acctgggccg taccgccttc ggagtacatg gcgaacagcg gctcagggcg cagtggcacc
     6781 ggcacggcgg cctgcgtcaa gccgctacca tgcgagggca gcaaagcgcc ctggggcacg
     6841 ggagccaacc ggcgcttggc cacacggtcg cgcagccagg tgcccagcag gtaaagtaga
     6901 ggcggcccca tgatgacgag gccggtccac ctgggaaagt tgtcgaacgg gggcgccatt
     6961 acggggaaca tgccgtacat agccatgctg ctcgcggtga ggatgaggga agcacgcatg
     7021 agcctgcccg tcccaatcag ggccaccggc accagcagcc aggaagcgta ccacggccag
     7081 taccagaccg aagcgaccgt caggtagacc aggagcaccc agccccaagc ccgcagcatc
     7141 tcaggaaagg tacgcgcgcc ccaggtacgc agaatggccc acgccgcggc gatcatgagc
     7201 ggcccccagc gcaggggcca gtcgagccgg gccgcaatcg cgtcaggctc ccagaacctg
     7261 taggggtacc agggttgggc gtgggcgaac gcgctcaccc actcggcccc ctggaacttc
     7321 agtatgtccg cgagcgagtt gaccgtgcgc gtggccgggg ggcccccggt gatggacctc
     7381 agcatgcccg gaccttccca gaatggtagg taggcgagcg ccaccagccc ggcgaacaga
     7441 cacgcagcct gcgtcatacg aaggatcccg ccctgccagg actcccggtc ccacggggga
     7501 cgctcggttc gcccctgcca gagcaacagc cacaggtagc ccggcaccag caccaccgcg
     7561 atcatcgtga ccagcacggc cgctgccaac gcgctcaccg ccaggggcca ccaccccttc
     7621 aagtgcatcc acaacgcgca caaaacgaaa gtgagcatca gtacgtcgtt gtggccgttc
     7681 gcgccaaatt ctatcagggc aagggggttc caggcatacg taacggtggc ggcgacctgc
     7741 atcgccccta tgtggtcggg gttcgacgtc gtggaggaga gtttgcgggc aaaggggatg
     7801 ccgtactttc gcagtgccag ccccg
//
LOCUS       DASRWZ010000628         6757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_119720, whole genome shotgun sequence.
ACCESSION   DASRWZ010000628 DASRWZ010000000
VERSION     DASRWZ010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119720"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1457
                     /locus_tag="VFH60_09970"
     CDS             78..1457
                     /locus_tag="VFH60_09970"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6314152.1"
                     /translation="MAQPQAQDGDMTSDTGTTMLPREGNRLWGRLGRLSGNPDFMRMW
                     AGETISMFGSQITYVAIPLTAVLVLHAQPGEMGILQAAGFAPFLLLTLFAGVWVDRNR
                     RRPILVLANAGRAVLLALVPLSALFGWLSMAQLFVISLLVGSLTVFFELAYQSYLPTL
                     VNKDELVEGNSKLQTSASVSSIGGPGIAGLLMEVLSAPFALLADAVSFLVSACMLLRV
                     RKVEPEPQRLHEPGSFRREMREGISIVFKNPYLRVFALEAASYNFFWNLMEAVYILYA
                     VRELGASPLVIGLVFSLGSIGSLVGALIAGRWANRFGIGRAITWSMALACASTLLVPL
                     AGDSVLGPWLVGASFFMGGLGVTVSVIHVVSLRQTVTPGHLLGRMNGSYRFITWGVIP
                     VGALLGGYLGEVLGLRAALAVGAAGMSVAWLWVLFSPVPRLRDLSDLEAADTQPEHPE
                     ATQLTDIAA"
     gene            1606..2307
                     /locus_tag="VFH60_09975"
     CDS             1606..2307
                     /locus_tag="VFH60_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET6314153.1"
                     /translation="MSEQVTFTRRGLVQGAVKILPIALVDVPFAIVLGVLARQAGLST
                     VEIMLMSMLVFAGASQLIAVGLWAFPVPVLTIVLTTLIVNLRHLLMGAALRPWFSRLG
                     PLKTYTSAHFITDESWALAMREFKNGGRDAAFLMGGGLMLFVLWVGGTGIGHLLGAGI
                     SAQDLVAWGLDFVVTAVFVALAVSLWRGKSDLLPWIVAAGVSLGAYLLLPGTRWYILL
                     GGLAGSIVGAFRNAD"
     gene            2297..2599
                     /locus_tag="VFH60_09980"
     CDS             2297..2599
                     /locus_tag="VFH60_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET6314154.1"
                     /translation="MQIDPVSLATILAMALVTYATRVGGLWLMGRVTPSPRVEAWLLN
                     IPGAVLVSIVTPLALSNGPADALAAGLTGVVAARTHNLLLAIGVGVGAAWVLRQLL"
     gene            complement(2591..3139)
                     /locus_tag="VFH60_09985"
     CDS             complement(2591..3139)
                     /locus_tag="VFH60_09985"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET6314155.1"
                     /translation="MEIVTDHLVLREFQESDFEAILAYQSDPGYLRYYERAWVENRAE
                     NTRNLLQRFLDSQQEQPRTKFQLAVTLREDGRLIGNVGVRKEDVEATQGDVGCELAPE
                     YWNLGYATEATRAMLSFGFEQLGLHRLWASTIADNRGAWRVLEKLGMTREGELRETTL
                     LADSWANSVVYGMLEQEWRALQ"
     gene            3892..4986
                     /locus_tag="VFH60_09990"
     CDS             3892..4986
                     /locus_tag="VFH60_09990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6314156.1"
                     /translation="MSSALKVEVAESFESAPGREEWDALLREAASSVVFLTKEWQQTW
                     WDSFASEHCCQLKIISVRTDGGALVGIAPLFVEQTPLPPLQAYERGVPRPEHGEGEPL
                     RLVRFVGGVEVADYLDIIAPAYRTREVWSALLDYLLQRRADWDALDLHSLPEFSPSRE
                     ALAQLAAEKGLHIEVVPEDVCPIIELPGDWETYLMGLRKKDRHELRRKVRKLEGRDDA
                     RWYLVRTDDPGELERGMRAFIDLHRSSGLDKAQFMDERRAEAFMEMAARMSRSGWLDL
                     AILAVHDEPAAAYLSFNYHERLYLYNSGYDPKFSAYSAGVALLAYRIHKAILQGVKTF
                     DFLRGDEDYKYDFGAKDTFVYRAVLRVASG"
     gene            5361..6626
                     /locus_tag="VFH60_09995"
     CDS             5361..6626
                     /locus_tag="VFH60_09995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6314157.1"
                     /translation="MCNIAMISMHTCPQACPDNLLGGRETGGMNVYIRELSRELSRRG
                     KKVDVFTRYQDPDTEPIHELGDGARVIHLEAGPKAPYDKNLLPNHVDEFVAQIEQFAL
                     TEGIRYDLVHSHYWVSGLVAQELRRKWGLPMVQMFHTLGVMKNLVAKSEADREVADRM
                     RVERQVMDWADRIIAATPLDKNQMLYCYGADGDKIAVIPAGVDTDHFYPRNKVQVRRQ
                     LGLPPDTPILLFVGRIERLKGIDTLLESAAVVSRTCAGRDLKVLIVGGGGQTPQENAE
                     IRRIWQLHQDLNLEDQVEFVGSKPQEMLPLYYSAADITVMPSHYESFGMVAVEAMASG
                     TPVIASNVGGLSYTVKDGVTGYLVPEENHFVLAEQVHHLLKNPDIREQMGRQAAKHAL
                     QFSWSNIATQIGDLYDTEIISNCGVACRC"
BASE COUNT         1247 a         2044 c         2134 g         1332 t
ORIGIN      
        1 ctataccgca cccccctcca agcatgactt cacctcgaaa ggaccgaaca ctctaccctt
       61 gcgaaggagc ttacacagtg gcacaacccc aagcgcaaga cggcgacatg accagcgaca
      121 ccggaacaac catgctgccg cgggagggca accgcttgtg ggggcggctt ggtaggctgt
      181 ccggcaaccc tgacttcatg cggatgtggg cgggcgagac gatctcgatg ttcggctcgc
      241 aaatcaccta cgtcgcgata cccctcaccg ccgtcctcgt gttgcacgct cagcctgggg
      301 agatgggcat cttgcaggcc gccgggttcg ccccgttctt gctccttacc ctgttcgccg
      361 gcgtgtgggt agaccgcaac cgccgccgtc ccatattggt gctggccaac gcgggccggg
      421 cggtgttgct ggcgctcgtg ccgctatccg ccctcttcgg ctggctgagt atggcgcagc
      481 tattcgtcat ctctctgctg gtaggctcgc tcaccgtttt cttcgagcta gcttaccagt
      541 cgtacttgcc caccctcgtc aataaggacg aactggtcga ggggaacagc aagctccaga
      601 cgagcgcgtc cgtatcgagc atcggagggc cgggcatcgc agggctactg atggaggtgc
      661 tgtcagcccc gttcgcgctg ctggctgacg cagtgtcgtt cctggtctcg gcctgcatgc
      721 tgctgcgcgt acgcaaggtc gagccggagc cgcagcgcct ccacgagccg ggtagcttcc
      781 ggcgcgagat gcgcgaaggc atctccatcg tgttcaagaa cccctacctt cgagtgttcg
      841 ccctggaagc ggcctcttac aatttcttct ggaacctgat ggaggctgtg tatatactat
      901 atgctgtgcg cgagcttggg gcctcgccgc tggttatcgg gttggtattc tcgctgggca
      961 gcatagggtc gttggtgggc gcgctcatcg caggcaggtg ggccaaccgc ttcggcatcg
     1021 gccgggcaat cacatggtcc atggcactcg cgtgcgccag cacactgctg gtaccgctgg
     1081 ccggggatag cgtgctaggc ccgtggctgg tgggcgcgtc cttcttcatg ggcggcctgg
     1141 gggtcaccgt gtcagtcatc cacgtggtga gcctgcggca gacggtcacc cctgggcacc
     1201 tgctaggccg catgaacggc agctaccgct tcattacctg gggcgtcatc ccggttgggg
     1261 cactgctcgg cggctacctt ggagaggtac tcggattgcg ggcagcgctg gcggtaggag
     1321 cggccgggat gtcggtagcg tggctgtggg tgctgttctc gcccgtcccc cgcctgcgcg
     1381 acctgtccga cttggaagcc gccgatacac agcccgagca ccccgaagcg acccaactga
     1441 ccgacatagc ggcgtgacga gtaatgagga ctgaggcacg agtggccccc taagtgaatt
     1501 atcccaatct ttgcgcgtgc tttgcgtccg ttgcgtccat tggggttcgc gtctcgttac
     1561 tcagtcctca ttactcatca ctcattacta tccccggaga gaaacgtgag cgaacaagtg
     1621 acattcaccc ggcgcgggct tgtgcagggt gcggtaaaga tactgcctat cgcgttggtg
     1681 gacgtgccgt tcgcgattgt gctgggcgtg ctggccaggc aggcgggcct ttccaccgtg
     1741 gaaatcatgt tgatgagcat gctggtgttc gccggggcct cacaactgat cgcggttggg
     1801 ctgtgggcct tcccggtgcc cgtgctcacc atcgtgctca ccaccctcat cgtcaacttg
     1861 cgccacctgc tgatgggcgc ggccctgcgg ccctggttct ccaggctcgg ccccctcaag
     1921 acctacacct cggcccactt catcacggac gaaagctggg ccctcgccat gcgcgagttc
     1981 aagaacggcg ggcgcgatgc ggcgttcctc atgggcggcg gcctcatgct gttcgtgctg
     2041 tgggtcggcg gcaccggcat agggcacctg ctgggcgcgg gcatcagcgc gcaggacctg
     2101 gtcgcgtggg ggttggactt cgtcgtgacg gcggtgttcg tggcgctcgc ggtatcgttg
     2161 tggcggggca agtcggacct gctgccgtgg atagtggctg cgggggtatc gctcggggcg
     2221 tatctgctgc tgcccggcac ccggtggtac atcctgctgg gcgggcttgc ggggagcata
     2281 gtcggggcgt tccgcaatgc agattgatcc cgtctcgctg gctaccatcc tggcaatggc
     2341 cctcgtcacc tacgccacac gcgtgggcgg cttgtggctg atgggcaggg tcacgccctc
     2401 gcctcgcgtg gaggcctggc tgctcaacat accgggcgcg gtgctggtct cgattgtcac
     2461 acccctcgcc ctgagcaacg gccccgccga cgctctggcc gccgggctaa ccggggtagt
     2521 ggccgcccgc acccacaacc tgctgctggc gattggggta ggggtaggtg cggcctgggt
     2581 gctgcggcag ctattgtagg gcgcgccatt cctgctccag cattccgtag accaccgagt
     2641 tggcccagct gtccgccagc agggtggtct cgcgtagctc cccctcacga gtcatgccca
     2701 gcttttccag cacgcgccac gcgcccctgt tgtccgcaat ggtcgaggcc cagaggcgat
     2761 gcaggccaag ctgctcgaag ccgaacgaca gcatggcgcg ggtggcttcg gtggcatagc
     2821 ccaggttcca gtattcgggc gcaagctcgc aacctacgtc gccctgagta gcctctacgt
     2881 cctctttgcg cactcccacg ttgccaatca gcctgccgtc ctctcgcaat gtcacggcta
     2941 actggaactt ggtgcgtggt tgctcctgct ggctgtcgag gaacctctgc aacagattgc
     3001 gcgtattttc agccctgttc tcgacccagg cgcgctcgta gtagcgcagg tagcctgggt
     3061 cggactggta ggccagtatc gcctcaaagt cgctctcctg gaactctctc agtacgaggt
     3121 ggtcggtgac aatctccatg tagcgcctcc ggggtaagac tgggtcgtgc ctgcgttatg
     3181 agccgggaag ccatctcaaa gtgaaggtgt cggatatcgg cagctaagcc ccgcctttgt
     3241 cattctgagc ccttcggctg cgctcagggt aaactccgcg aagaatctcg tcctctaccc
     3301 tgatgtttcc cttttccgta gcacatggca ccttaccggc gtgaggaaag gtggcatttg
     3361 ggtgctaagg gacgagattc ttcgctacgc tcagcagagc atccggtaag taagtaccca
     3421 acgcaggagc aaagtggccc ttgtcattcc gagcgtagcg aggaatcagg tggcagtcgt
     3481 actcagccgg attgcagcag gcgagtggcg agcgcacacc cgtacccagc actgagtaca
     3541 gctatgccac ttcttcccta cccacctgat tcctcgctac gctcggaatg acaaggacca
     3601 ctttgcccct gctgttaggc acttacttac cggacgcacc acccataatg acaaaggcgg
     3661 tcttggtctc caggttccca ttatcgtcct cgagatagca tccagtttat ccgcctgttc
     3721 aacatgcgca tactcccatg tggcggcacg cggactgctc tttagaatct cggtcctcag
     3781 accgattaga accgggtgcg aaaataggat ataattaaac tgttgaaccg taaaccaatt
     3841 ttaggcgtgt atataatcgt acagcataaa ggaccgcatg ggatggtgtt tgtgtcttca
     3901 gcgttgaagg tagaagtggc tgagagcttc gagtcggctc ccggccgcga ggaatgggat
     3961 gcattgctgc gcgaggcggc ttccagcgtg gtgtttctca cgaaggaatg gcagcagaca
     4021 tggtgggaca gcttcgcttc ggagcactgt tgccagctca agataatttc ggtccggaca
     4081 gacggaggag cgcttgtggg catcgctcct ctttttgttg agcagacgcc cctgccaccc
     4141 ctacaggctt acgagcgagg agtgcccagg ccggaacatg gcgagggcga gccgctgcgg
     4201 ttggtgcgct tcgtgggcgg cgtggaagtg gccgactacc ttgacataat tgctcctgcc
     4261 tacaggacgc gggaggtgtg gtctgcgctg ctggactacc tgttgcagcg tcgtgccgac
     4321 tgggatgcgc tagacctcca ctcgctgccg gagttctccc cctcccgcga ggcgctggct
     4381 cagctagccg cggagaaggg gctgcacatc gaggtggtgc cggaggacgt ctgccccata
     4441 attgagttgc cgggcgattg ggagacttac ctgatgggcc tgcgcaagaa ggaccgccac
     4501 gagctgcggc gcaaggtccg caagctggag ggccgcgacg acgcccgctg gtacctggtc
     4561 aggacggacg accccggcga gttggagcgg ggcatgcggg cctttatcga cctgcatcgc
     4621 tccagcggcc tggacaaggc gcagttcatg gacgagcggc gagccgaggc cttcatggag
     4681 atggcggcgc gcatgtcccg tagcggctgg ctcgacctgg ctatcctggc cgtgcacgac
     4741 gagcctgccg ccgcctacct atcgttcaac taccacgaac gcctctacct ttacaactcc
     4801 ggctacgacc ccaagttctc ggcctacagc gcgggtgtcg ccctgctggc ctaccgcatc
     4861 cacaaggcca tcctgcaagg cgtaaagacc ttcgacttcc tgcgcggcga cgaggactac
     4921 aagtacgatt tcggagccaa agacaccttt gtgtaccggg cggtgctgcg agtggctagt
     4981 ggttagtgtt ggctgaataa gggcaggaga ttggaactgt gctcccccct gtcatcctga
     5041 gcgaagcgaa ggatctaaag aaggtgagta ggggatgctg ttgaatggtg ggaataatgg
     5101 tgctgacggt tcagcatacg ggaagtccaa catcttagat ccttcactgc gttcaggatg
     5161 acagaggggg gcagggtcgc cgctattggc acggggtcgg gtccactgcg acacagtgcg
     5221 tgcgatgcgc gcagtggctt gcaaagtgct aatggagcgt aacaggagct aacaaggtcg
     5281 cacggcaagg aagcccactc cacatgtcgt tttcaccctg tgatgagaat gtatacaaat
     5341 ttagtaggaa aattagataa atgtgcaaca tagcaatgat tagcatgcat acctgcccgc
     5401 aggcttgtcc cgacaacctg ctgggggggc gcgagacggg cggcatgaac gtctacatcc
     5461 gcgagcttag ccgcgagctt agcaggcgcg gtaagaaggt ggacgtattc acccgctacc
     5521 aggacccgga caccgagccg atccacgagc ttggcgacgg cgcgcgggtc atacacctgg
     5581 aagcagggcc gaaagccccg tacgataaga acctgctgcc caatcacgtg gatgagttcg
     5641 tggcccagat cgagcagttc gccctcaccg aaggcatccg gtacgacctc gtgcacagcc
     5701 actactgggt gtccgggctt gtggcgcagg aactacggcg caagtggggc ctgccgatgg
     5761 tgcagatgtt ccatacgctg ggcgtgatga agaacctcgt cgccaagtcg gaggccgacc
     5821 gcgaggtggc agaccggatg cgcgtcgagc ggcaggtgat ggactgggca gaccgcatca
     5881 tcgccgctac gcccctggac aagaaccaga tgctctactg ttacggggcg gacggtgaca
     5941 agattgccgt catcccggcg ggcgttgata ccgaccactt ctacccacgc aacaaggtcc
     6001 aggtacgccg ccaattgggc ctgcctccgg atacccctat cctgctcttc gtgggccgca
     6061 tagagcggct gaagggcata gatacgctgc tggaatcggc tgccgtggtg agccgcacct
     6121 gcgccgggcg cgacctgaaa gtgctcatcg tgggtggggg cggacagacg ccgcaggaga
     6181 acgccgaaat ccgccgcatc tggcagttgc accaggacct caacctggag gaccaggtgg
     6241 agttcgtcgg ctccaaaccc caggagatgt tacccctcta ttactcggcg gccgacatca
     6301 cggtaatgcc ctcgcactat gagtcgttcg gcatggtggc ggtcgaggcg atggcctcgg
     6361 gcacccccgt aatagcctcg aacgtcggcg gcctctcgta cacggtgaaa gacggcgtaa
     6421 ccggctatct tgtgcccgag gagaaccact tcgtgctggc cgagcaggtg caccacctcc
     6481 tcaagaaccc cgatatccgc gaacagatgg gccgccaggc agccaagcac gccctacaat
     6541 tctcctggtc caacatcgcc acccagatag gcgacctgta cgacaccgaa atcatctcca
     6601 actgcggcgt cgcctgccgg tgctagcggg gtaaggagac gaaaacgcaa agcatgccct
     6661 gagcgaagcc gaagggacgc gaagaacgca aaggacgcgc aaagattagg attgtttagt
     6721 gagtaagcaa acggagcagg gtacgacatc ccgtacc
//
LOCUS       DASRWZ010000629         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1352546, whole genome shotgun sequence.
ACCESSION   DASRWZ010000629 DASRWZ010000000
VERSION     DASRWZ010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352546"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VFH60_10000"
     CDS             complement(<1..643)
                     /locus_tag="VFH60_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6314158.1"
                     /translation="MCYDDKARPPMPPTNGGRAQGQDLVLTAADGNQFAAFLAQPESP
                     TGAQILIYPDVRGLHGFYKDLALRFAETGVRALAIDYFGRTAGLAPRDDSFEFMPHVQ
                     QLSISNIFADAQAALDYLRQGEGADRPAVVVGFCIGGSLTLLTAGQDLGLSGVIAFSA
                     GMSRDFGGFGTALDRAAHVKYPVLGLFGGADPGIPPEQVAELDGRLGQTGVPHD"
     gene            complement(998..2026)
                     /locus_tag="VFH60_10005"
     CDS             complement(998..2026)
                     /locus_tag="VFH60_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6314159.1"
                     /translation="MQAVRLVEVREPLRMQDIPVPAVGDGEVLIRVRAAGICHSDVHY
                     RAGTSPVQPLPRTLGHEVAGVVEQVGEQVAAVSVGDRVCVHYVLSCGQCHYCRGGNEQ
                     FCVQYKMVGRHAEGGYAEYIAVPEHNAVRLPDEVSFEHGAVLMCSSATSFHALRKSRL
                     RAGESVAVFGVGGLGISAVQLAFAFGALDVYAVDINPDKLALAEKYGAVAVNAAAVDP
                     VTEIRERTQGRGVDVALEVIGLALTMKQAVRSLANLGRAVVAGISERPLELDTYNELL
                     GREAEIIGTNDHLLQELPLLLELARRGKLDLSEAVTRTVPLDASAINGVLDNLEHFRS
                     DVRTVIVP"
     gene            complement(2028..2474)
                     /locus_tag="VFH60_10010"
     CDS             complement(2028..2474)
                     /locus_tag="VFH60_10010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET6314160.1"
                     /translation="MSVFTPAEIEYLSSQRLCRLATIGRGGEPHVVPVRFSYNADLDV
                     IELGGGDFGSSKKFRDMLSNPAVALVVDDLTPPPVRPRGVEGRGRAEALLTGGDSVWP
                     GADPEYVRVSPTYIASWGIDGDPYSPAGRAAHPVSPAPTQTEEGAP"
BASE COUNT          472 a          868 c          839 g          480 t
ORIGIN      
        1 tgtcgtgcgg cacgccggtc tggccgaggc ggccatcgag ttccgcgacc tgctccggcg
       61 ggatgccagg atcggcccct ccaaagaggc caagcaccgg gtacttcaca tgggcagccc
      121 ggtcgagcgc cgtgccgaag ccgccaaagt cgcgcgacat accggcagaa aaggcgataa
      181 cacccgacag accgaggtcc tgcccggcgg tcaaaagtgt caggctgccg cctatgcaga
      241 agccgaccac gacggctggc cggtccgccc cctccccctg ccgcaggtaa tcgagggccg
      301 cctgtgcgtc cgcgaagatg ttggagatgc tcagctgttg aacgtgaggc atgaactcga
      361 acgaatcgtc gcggggggcc agcccggcgg tgcgcccgaa gtagtcgata gccagcgccc
      421 gtaccccggt ctccgcgaac cgcaaggcaa ggtccttata aaagccgtgc aggccgcgaa
      481 cgtcgggata aatgaggatt tgcgcgccgg ttggactctc aggctgcgcg aggaaggcgg
      541 cgaattggtt gccgtcggcg gcggtaagta ccaggtcctg gccctgcgcc cggccgccat
      601 tcgtgggcgg catggggggc cgggccttgt cgtcgtagca cataagggtc tacctccggg
      661 atttgggccg gtgcgagggc aagctccccg cccggctgtc ttactatgat aatcgattcc
      721 gcgctctaag tgcgatcagg ctgagcaaag gaatgcagtg atagtccaga gcgtatctgc
      781 aaagtgccgc atgcgggaac cctctcccac tttgtcattc tgagcgcagc gaagaatctc
      841 gtcctccagc acccaaatac cacctttctt cactctacct gttgctatgg tgctacggaa
      901 aaggggaaca tcccggtaga ggacgagatt cttcgctgcg ctcagaatga caaaggtccc
      961 gcttgggtgc ttgtctccca gcgtttgcag ataccatcta gggcactatg acggtgcgca
     1021 cgtcgctacg aaagtgctcg aggttgtcga gcacgccgtt gatggcgctg gcgtcgaggg
     1081 gcactgtacg cgtgacggcc tccgatagat cgagcttccc gcgccgcgcc agttccagca
     1141 agaggggcag ttcttgcagc aggtggtcgt tggtgccgat gatttcggcc tctctgccga
     1201 gtagctcgtt gtaggtatca agctcaagcg gccgctcgct gatgccggcc acaaccgccc
     1261 gccccaggtt cgcgagggac cgcaccgcct gcttcatcgt gagggcaagg ccaatcacct
     1321 ccagggcgac atcgacgccc ctgccctggg tgcgctcacg aatttcggta acgggatcga
     1381 ctgctgccgc gttcaccgcg accgccccgt acttctcggc cagcgccagc ttgtcggggt
     1441 tgatatcgac cgcgtacacg tcgagcgcgc cgaaggcgaa cgcaagttgc accgccgata
     1501 tgcccaggcc gcccacgccg aacacggcca cgctctcccc ggcccgcagc ctggacttgc
     1561 gcagggcgtg gaacgaggtg gccgaggaac acatcaggac agctccgtgc tcgaaggaga
     1621 cctcgtccgg caggcgcacg gcgttgtgtt cgggtacggc gatgtattcg gcgtacccgc
     1681 cctccgcgtg acgccccacc atcttgtact gcacgcagaa ttgctcgttt ccgcccctgc
     1741 aatagtggca ctggccgcag ctcagcacgt agtgcacgca cactcggtct cccacgctca
     1801 cggcagcgac ctgctcgcct acctgctcga ccacacccgc cacttcgtgc ccaagagtcc
     1861 gaggcaacgg ctgcacgggc gaggtgcccg cccgatagtg cacgtctgag tggcagatac
     1921 cggcggcgcg cacgcgtatc aggacttcgc catctccaac cgcgggcacc gggatgtctt
     1981 gcatgcgaag gggttcgcgg acctcgacca ggcggacggc ttgcatgcta gggtgctcct
     2041 tcttcggtct gcgtgggtgc gggtgagacg gggtgggccg cgcgcccggc ggggctataa
     2101 gggtcgccgt cgatgcccca ggacgctata taagtggggc tgacgcgcac gtattcgggg
     2161 tccgcgccgg gccagacgct gtcgccccct gtcagcagcg cctcggcccg ccctcgccct
     2221 tccacgcccc tgggcctgac ggggggcggc gtgaggtcgt ccaccacgag ggccacggcg
     2281 gggttgctca gcatgtcgcg gaacttcttg ctgctgccga agtcgccccc gccaagctct
     2341 ataacgtcca ggtcggcatt gtagctgaag cgcaccggca ccacgtgcgg ctcgccccct
     2401 ctgccaatcg tagccaaccg gcacaagcgc tggctgctca gatattcgat ctcagccgga
     2461 gtaaagacgc tcaatattac ctccagggct gcgtggtgtg aagaagatgc atggagggga
     2521 attatatcat gcgagtgtcc tacattccca ggcggtgctt gtgtctcccc cgtcgctgtg
     2581 cttatccggc aggagctttt catttactgc cccggctgat agaataccta atggattgac
     2641 cgccaagttc accccctgt
//
LOCUS       DASRWZ010000630         1767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1285228, whole genome shotgun sequence.
ACCESSION   DASRWZ010000630 DASRWZ010000000
VERSION     DASRWZ010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285228"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1534)
                     /locus_tag="VFH60_10015"
     CDS             complement(167..1534)
                     /locus_tag="VFH60_10015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6314161.1"
                     /translation="MTFKSFNLDPRIMSGVQALGYEIPTPIQNQAIPPVMKGRDVMGL
                     AQTGTGKTAAFVLPILQRLLNGPRGHVRALVLAPTRELAEQIDEAIKTLGQQTGLRSV
                     TLYGGVGIHPQIQTLRKGVEIVVACPGRLLDHISQRTITLANLEVLVLDEADHMFDMG
                     FLPDVRRILKHVPAVRQTLLFSATMPDDIRHLAHDVLRDPVTVQVGPSAPVATVSHAL
                     YPVPAHLKTDLLMELLKHTDTESVLIFTRTKHRAKRLGENLEKAGYSATSLQGNLSQN
                     RRQAALDGFRDGKYQIMVATDIAARGIDVTRISHVINYDIPGTADAYTHRIGRTGRAA
                     KTGDAFTLVSEEDAVMVRTIERVLGAKVERRTIAGFDYGQHAPARSNEFARPPREPQR
                     RRPQPSAETARPQPATTTSHRSSEDRRPNGNQSGRRYSNPAPSGSGFRGRDERRSRNT
                     SRASA"
BASE COUNT          300 a          507 c          607 g          353 t
ORIGIN      
        1 agacaccctc tcaggatgac agaggggggc tttgggtgcc cggcaagtgc ggtgtacagt
       61 ctgctttcca aagtggctcg aaacgaaaaa ggagtgatga gtcgaattca tcactccttt
      121 ttcgtttctt cgcgtgctcc cggtgctatt cagccacccg gtacttctag gccgaggcgc
      181 ggctcgtgtt gcgagaacgg cgctcatcgc gacccctgaa gccggagccg ctgggtgctg
      241 ggttggaata acgcctgcct gattggttgc cgttcgggcg acggtcctcg gaagacctgt
      301 ggctcgtagt cgtagcaggc tgcggccttg ccgtctcggc ggagggttgc ggtcggcggc
      361 gctgcggctc tcgcgggggg cgggcgaact cattgctgcg cgcaggggcg tgctgcccgt
      421 agtcgaagcc cgcgattgtg cgacgctcca ccttcgcgcc cagcacccgc tcgatcgtgc
      481 gcaccatcac cgcgtcctct tcggacacga gggtgaaggc atcgccagtc ttggcggcgc
      541 ggcccgtgcg gcctatgcgg tgcgtatacg cgtcagcggt gccgggaatg tcgtagttga
      601 tgacgtgcga gatgcgcgtt acgtcgatac cccgcgccgc gatgtcggta gccaccataa
      661 tctggtactt gccgtcccgg aagccgtcaa gcgcggcctg gcggcggttc tgcgagaggt
      721 tgccctgcag cgaggtggcg ctgtagcccg ccttctccag gttctcgccc aggcgcttgg
      781 cccggtgctt ggtgcgggtg aagatcagca ccgactcggt gtcggtatgc ttgagcagtt
      841 ccatcagcag gtctgtcttg aggtgcgcgg gcacggggta gagcgcgtgc gaaacggtcg
      901 ctacgggcgc gcttggcccc acctgcacgg tcaccggatc gcggagcacg tcgtgcgcca
      961 ggtgccggat gtcgtcgggc atggtggccg agaagagcag ggtctggcgc acagccggga
     1021 cgtgcttcag gatgcggcgc acatcgggca agaagcccat gtcgaacatg tggtcggcct
     1081 catcgagcac gagcacttcc aggttcgcca gggtgatggt gcgctgcgag atatggtcga
     1141 gcaggcggcc ggggcaggcc accacgattt cgacgccctt gcgaagcgtc tggatttgcg
     1201 ggtggatgcc cacgccgccg tagagggtca cgctgcgcag tcccgtctgc tggcctagcg
     1261 tcttgatagc ctcatctatt tgttcggcaa gctcgcgggt aggggcgagc accagggcgc
     1321 ggacgtggcc acgcgggccg tttagaagtc gctgcaaaat aggcagcaca aaggccgcgg
     1381 tcttgccggt gccggtctgg gcgaggccca tcacgtcgcg gcccttcatg acgggcggta
     1441 tggcctggtt ctgaatgggg gtaggtatct cgtacccgag ggcctgtacg cccgacatga
     1501 tgcgggggtc gagattgaac gatttgaaag tcactgtaag tcctttatga gaaagcggtg
     1561 ccggtcgtct aaacaaccgc ataaaaaatg agccttcgga cgaccgggct cacacagaag
     1621 cgttatgctt ttggcctgta tattatcgac ataagtatta tacccgcttg aaggcaaaag
     1681 ttcaagtcgc cgggttgcga gcggcctggc tgcaagccac aaagagttaa aggtccggac
     1741 gtggcccctc cttgccattc agagttc
//
LOCUS       DASRWZ010000631         6559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_256506, whole genome shotgun sequence.
ACCESSION   DASRWZ010000631 DASRWZ010000000
VERSION     DASRWZ010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256506"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1001
                     /locus_tag="VFH60_10020"
     CDS             15..1001
                     /locus_tag="VFH60_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6314162.1"
                     /translation="MANVDETNVAVIEQPGTGEGLVIEDLHVNVEGREILKGVSLTVG
                     RGEVHALMGPNGSGKSTLVNALMGNPNYEITSGRATLDGVDLLDLDPDERAKVGLFLG
                     FQYPSSIPGVSVANFIRTAMVSLTKPRARSEQDLLTETGDIRRDAEPAPAPSSLGLAE
                     ANYVGRGGVRKSNPVVKEFRKNLREKLALLKMDESFATRYVNDGFSGGEKKRLEILQM
                     AMLKPKIAMLDEPDSGLDIDAVRVVAEGINSLRGPGLGTLIVTHYQRILNYIKPEFVH
                     VMVNGRIVQSGGPELAQQLEAQGYDWLRPDEGPVEDRTDGGRRDVEGEYDNA"
     gene            988..2379
                     /gene="sufD"
                     /locus_tag="VFH60_10025"
     CDS             988..2379
                     /gene="sufD"
                     /locus_tag="VFH60_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET6314163.1"
                     /translation="MTTPSVVRPSDLVADRPGLSMEAVVALSEARNEPQWLRESRLEG
                     WQAFETLPTPRWTKGIAQWWTTDVSDISLDEFQPYVPSNRTAEEARNLANLGGESTEE
                     GSLLVQVNSEVAYLYVSEDMKAQGVVFCSLEDAVRDHPELVQKYLHKLVAPNTDKFAA
                     LHTALWSGGVFLYVPQGVHVELPVHVVYRLDTPGAVSLGHTLVVAEKGSSVRYIEEFH
                     SEGQTGQALHSGVVEVAVGEEAKVEFTSMQEWARNVYSFATRYATVDRGGLMHWVIGE
                     VGAKLLKAHAETQLLGTGARTLTRGIAFADSDQHFDNTSNTRHAAVDTFGDILFKGAV
                     RDVARLGFEGIIKVEHGAQRTDSYLTMNTLFLSEGSKASSIPGLEILADNVKCSHGAT
                     VGTVQEQEVFYLMSRGIDRISAEKLIVGGFFEPVIAEMPLESVRQRLRDIVEQKVGLP
                     RVGKAARRPGEGP"
     gene            2735..2947
                     /locus_tag="VFH60_10030"
     CDS             2735..2947
                     /locus_tag="VFH60_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET6314164.1"
                     /translation="MPRKIRELKADLRRAGFNYRAGKGSHTIWFHEIIPNEITLSGND
                     GDDAKPYQERLVRNILHTLNEAQRRN"
     gene            2947..3192
                     /locus_tag="VFH60_10035"
     CDS             2947..3192
                     /locus_tag="VFH60_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET6314165.1"
                     /translation="MNEDKITEPYARYSMIIEWSNEDEAYIVTVPELPGCVTHGNTYE
                     EAVRQGQDAIEGWIDVAQELGRPVPAPKVRENVTTQP"
     gene            3230..3862
                     /locus_tag="VFH60_10040"
     CDS             3230..3862
                     /locus_tag="VFH60_10040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6314166.1"
                     /translation="MDQPTERADRKLLVEQGYDKIAWDYLATKPGDAAQVLSTLILMT
                     TDLHDGAKVLDLGCGAGVPSTQFLSRRFDVTGVDVSAQQLELARTYAPNATLIKSDMV
                     ALSFPEATFDLVVAFYSIIHVPREEQPALVGNIFTWLKPGGRFLATWPLVEWEGEEKD
                     WQGWGGTMWWSHFGGADNLRMLRGAGFIIESVEEHVGEEDWLWVVARKEQ"
     gene            3911..5275
                     /locus_tag="VFH60_10045"
     CDS             3911..5275
                     /locus_tag="VFH60_10045"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET6314167.1"
                     /translation="MVTNIAREATEQDGKVSTAEIAASDPRNVAAPPPPGAAPTPAPG
                     AQAGAVDWERVRADFPLLRRTLHGKRLVFLDSTATSQKPQAVLDAMDDYYRTTNANIH
                     RGVYELSEVATDRYEKARARVARFINAKSSREIIYTRNATEAINLVAYSWARANLVPG
                     DVVLLSKMEHHSNLVPWHMLAAERGITLRYLDVDAAGLLVLDSLDAQLEGVKLLSVTH
                     MSNVLGTINPVEELIPRAHAAGALVMLDAAQSVPHLPVDVQALDVDFLVFSGHKMCGP
                     TGIGVLYGKRALLEAMPAFLGGGDMIGTVTLEGFTPNELPWKFEAGTPAIAEAIGLGA
                     AVDYLSGLGMANIHRHEQELTAYALDSITPMRGVKVYGPPAERKGGVVSFEIEGVHPH
                     DIATILDGEGICIRAGHHCCQPLMHHLAVPALARASFYLYTTTEDVDALVEGIVKVQS
                     IFGV"
     gene            5312..5725
                     /locus_tag="VFH60_10050"
     CDS             5312..5725
                     /locus_tag="VFH60_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314168.1"
                     /translation="MASLLFLISLSVLLSGVGGFLFLSQLLTVMSPAAGGMASGDTLR
                     LVYFALVFVGSLAILLRVGLLARNTVGGRVPVHLYMREAAIYGAVMLLVLGMSCWLVS
                     EWARQLERGGLQLIDIAGRGLESEDLTLFSYQDWQ"
     gene            5803..>6559
                     /locus_tag="VFH60_10055"
     CDS             5803..>6559
                     /locus_tag="VFH60_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314169.1"
                     /translation="MTATLVGGWLGSLLSLYVYWNGISFYPLFSVVPFIGSTLLGLVL
                     GVFQGRALRNSLGAQVWPHWALMTWLGLILSIGVGGIAGPQTDRIITSLSDISTEAAQ
                     FGGVILFSTLQALVVGGLQCIILWSHTRKAAWWPLGLAAGLVVGNCIALEVSILAIAS
                     IIGPDPTMFAVPYMFFISGVSSACIPWPFTAVITGWVLVNLLKNPEKQSTGISSNYIM
                     TKFERLYSELERRYGPASPELKPLMAAFFDALMA"
BASE COUNT         1321 a         1949 c         2099 g         1190 t
ORIGIN      
        1 ggcataataa cagtatggcg aatgtagatg agaccaatgt ggcggtgatt gaacagccgg
       61 gcaccggaga gggcctggtt atagaggacc tgcacgtgaa cgtggagggc cgcgagatac
      121 tcaagggcgt gagcctcacc gtgggccggg gcgaggtaca cgcgctcatg ggccccaacg
      181 gttcgggcaa atcgaccctg gtgaacgcgc tcatgggcaa ccccaactac gagattactt
      241 caggtagggc caccctcgac ggcgtggacc tgctcgacct cgaccccgac gagcgggcca
      301 aggtgggcct cttcctcggc ttccagtacc cctcctccat ccccggcgtg agcgtcgcca
      361 acttcatccg cacggcgatg gtctccctga ccaagccccg cgcccgcagc gagcaggacc
      421 tgctcaccga gacgggcgac atccgccgcg acgcggaacc ggcaccggca cccagttccc
      481 tggggcttgc ggaggccaat tatgtgggcc gtggcggggt gcgcaagtcg aaccccgtcg
      541 tcaaggagtt ccgcaagaac ctgcgcgaga agctggccct gcttaagatg gacgagagct
      601 tcgccacccg ctacgtgaac gacggcttct ccggcggcga gaagaagcgc cttgaaatcc
      661 tccagatggc gatgctcaag cccaaaatcg ctatgctcga cgagccggac tccggcctcg
      721 acatcgacgc ggtgcgcgtg gtggccgagg gcatcaattc gctgcgcggg ccgggccttg
      781 gcaccctgat cgtcacccac taccagcgca tcctcaacta catcaagccg gagttcgtgc
      841 acgtcatggt gaacgggcgc atcgtgcagt cgggcggccc cgaactggcc cagcagcttg
      901 aggcccaggg ctacgactgg ctgcgccccg acgaaggccc ggtagaggac cgcaccgacg
      961 gcggcaggcg cgacgtggag ggcgaatatg acaacgccta gcgtcgtccg gccctcggac
     1021 ctggtggcgg accggcccgg cctgagcatg gaggcggtag tcgccttgag cgaggcccgc
     1081 aacgagcctc agtggctccg cgagtcgcgg ctcgagggct ggcaggcgtt cgagaccctg
     1141 cccaccccgc gctggaccaa gggcatcgcc cagtggtgga ccaccgacgt gagcgacatc
     1201 agcctggacg agttccagcc ctacgtgccc tccaaccgca ccgccgaaga ggcccgcaac
     1261 ctggcgaacc tgggcggaga gagcacggaa gagggcagcc tgctggtgca ggtcaactcc
     1321 gaggtggcct acctttacgt gtccgaggac atgaaagcgc agggcgtggt cttctgctct
     1381 ctggaagacg cggtgcgcga tcaccccgaa ttggtgcaga agtacctcca caagctcgtg
     1441 gcccccaaca ccgacaagtt cgcggcgttg cacacggcgc tgtggagcgg cggggtattc
     1501 ctctacgtgc cgcagggcgt acatgtggag ttgccggtgc acgtggtcta tcgcctggac
     1561 acgccgggcg cggtatcgct ggggcacacg ctggtagtgg ccgaaaaagg ctcgagcgtg
     1621 cgctacatcg aggaattcca ctcggagggg cagacggggc aggcgctgca ttcgggcgtg
     1681 gtagaggtag ccgtgggcga agaggcgaag gtcgagttca cttcgatgca ggagtgggcg
     1741 cgcaacgtct actcgttcgc cacccgttac gctacggtgg accggggcgg cctgatgcac
     1801 tgggtcatag gcgaggtggg ggccaagctg ctcaaggccc acgccgagac gcagctgctt
     1861 ggcaccggcg cgcgcaccct caccaggggc atcgccttcg ccgacagcga ccagcacttc
     1921 gacaatacct ccaacacccg gcacgctgcg gtggacacgt ttggcgacat cctgttcaaa
     1981 ggtgccgtgc gcgacgttgc ccgcctcggc ttcgagggca tcatcaaggt ggagcacggc
     2041 gctcagcgca ccgactccta cctcacgatg aacaccctgt tcctcagcga aggctccaag
     2101 gccagttcca tccccggcct ggaaatcctc gccgataacg tgaagtgctc tcacggcgcg
     2161 acggtcggca ccgtgcagga gcaggaggtc ttctacctca tgtctcgcgg catcgaccgc
     2221 atcagcgccg agaagctgat cgtgggcggc ttcttcgagc cggtaattgc cgagatgccc
     2281 ctcgaatcgg tgcgccagcg cctgcgcgac atagtcgagc agaaggtggg cctgccccgt
     2341 gtcggcaaag cggcaaggcg gcccggcgaa gggccgtagg gggacggagt aatgagggct
     2401 gaggactgag aactgagtga cgatgggtgg ctgcgagcgc gtggtcgccc attgttgcat
     2461 ctgtggaggt cgtgtagggt gagcatgaag aagggtacgt gccctgaatg tggctccaat
     2521 gatgtgcgtt acgatagagg atcgtatagt gaagcgattt ataccagggc aaacaacata
     2581 ataatgatcc ataagcccgg attgatgggc atgaacgtcg agagatcata cctggattcg
     2641 tacgtatgcg tgaactgtgg ctacgtgcgc agctttgtcg cagaccggca aaacctggat
     2701 accatagcga agcactggcc gagggtggtg gaagatgccc aggaagatac gcgagctgaa
     2761 ggctgatttg cggcgggcag ggttcaacta tcgggctgga aaaggcagcc ataccatctg
     2821 gttccatgaa atcattccga atgagataac cttgtccggt aatgatggcg acgatgcaaa
     2881 gccttatcag gaaaggctcg tgcgaaatat tttgcacacg ctaaacgagg cgcaaaggag
     2941 gaattgatga atgaggataa gataactgag ccatatgcgc ggtactcaat gattatcgaa
     3001 tggtccaacg aagatgaagc atacatagtt accgttcctg agttgcccgg ttgtgtaaca
     3061 cacggcaata cttacgagga agcggtgcgt caagggcaag atgccataga aggctggatt
     3121 gatgtcgccc aggaactagg ccgcccggtt cctgctccta aagttagaga gaatgtaaca
     3181 actcaaccat agttgtatgg cagctctaag gttgttgccg agggaaacaa tggaccaacc
     3241 gaccgaacga gcagaccgca agctactggt cgaacagggc tacgacaaga tagcctggga
     3301 ctaccttgcc accaaaccgg gggacgcagc ccaggtactc tccaccctca tcctgatgac
     3361 caccgacctg cacgacggcg ctaaggtgct cgacctcggc tgcggggcgg gcgtaccctc
     3421 tacgcagttc ctttcccggc gcttcgacgt gaccggggtg gacgtttcgg cgcaacagct
     3481 tgagctagcc cgcacctacg cgccaaatgc caccctcatc aagtcggaca tggtagccct
     3541 cagcttcccg gaagccactt tcgacctggt cgtggccttc tactccatca tccacgtgcc
     3601 acgcgaggag cagcccgcgc tggtgggtaa catttttaca tggctcaagc ccggcggcag
     3661 gttcctggcg acgtggcccc tggtcgaatg ggagggcgag gagaaggact ggcagggctg
     3721 gggcggcacg atgtggtgga gccatttcgg cggtgcggat aacctccgga tgctgcgagg
     3781 ggctggattc ataatagagt cggtcgaaga gcacgtaggc gaagaggact ggctctgggt
     3841 ggtcgccagg aaagagcagt aacggcaatg atggcagtga tggcaatgat gctgaaagca
     3901 gggtagcagt atggtaacga acatcgcacg cgaagcaacc gagcaggacg gcaaagtctc
     3961 cacggccgag attgccgctt ccgacccgcg caacgtagcc gcgccacccc caccgggagc
     4021 ggcacctaca cccgccccag gagcgcaggc aggggccgtc gattgggagc gggtgcgcgc
     4081 agacttcccc ctcctgcggc gcaccctgca cggcaagcgg ctggtcttcc tcgacagcac
     4141 cgccacctcg cagaagccgc aggccgtgct cgatgcgatg gacgactact atcgcaccac
     4201 caacgccaac atccaccggg gcgtgtacga gctgagcgag gtagccaccg accgctacga
     4261 gaaagcccgc gcccgcgtcg cccgcttcat caacgccaaa agcagccgcg agatcatcta
     4321 cacccgcaac gccaccgagg cgatcaacct ggtggcctat agctgggcgc gcgccaacct
     4381 cgtgcctggc gacgtggtgc tgctgagcaa gatggagcac cattccaacc tggtgccctg
     4441 gcacatgctc gccgccgagc gtggcataac cctgcgctac ctcgacgtgg acgcggccgg
     4501 gctgctggtg ctggacagcc ttgacgcgca gcttgagggc gtgaagctgc tgagcgtgac
     4561 ccacatgagc aacgtgctgg gcaccatcaa cccggtggag gagcttatac ccagggcgca
     4621 cgcggcaggg gcgctggtaa tgctcgacgc ggcccagagc gtgccccact tacccgtgga
     4681 cgtgcaggcg ctcgacgtag acttcctcgt cttctccggg cacaagatgt gcggccccac
     4741 cggcataggc gtgctgtacg gcaagcgcgc cctgcttgag gcaatgcccg cgttcctggg
     4801 cgggggcgac atgataggca ccgtgacgct ggaaggcttc acgcccaacg agctaccctg
     4861 gaagttcgag gcaggcaccc cggccatcgc ggaggctatt ggcctgggcg cggcggtgga
     4921 ctacctatcc ggcctcggca tggcaaacat ccaccggcac gagcaggagc ttaccgccta
     4981 cgccctcgat tccattacgc ccatgagggg agtaaaggtg tacggcccgc ccgccgagcg
     5041 caaggggggc gtggtgtcgt tcgagataga gggcgtccac ccgcacgaca tcgccaccat
     5101 cctggacggc gagggcatct gcatccgcgc cgggcaccac tgctgccagc cgctgatgca
     5161 ccacctcgcc gtgcccgccc tggcgcgcgc ctccttctac ctctacacca ctaccgagga
     5221 cgtagacgcg ctggtggagg gcatcgtgaa ggtccagtcg atattcggcg tataggcagc
     5281 ctggggtatt acgagtttgt acgcgagggt aatggcgagt ctgctgttct tgataagcct
     5341 ctccgtgctg ctgtcggggg tgggcggctt cctgtttctg tcgcagttgc tcaccgtgat
     5401 gtccccagcc gccggtggca tggcaagcgg cgacacgttg cgcctggtct acttcgcgct
     5461 cgtcttcgtt ggcagcctgg ctattctcct gcgggtgggc ttgcttgccc gcaacacggt
     5521 gggcggcagg gtgcccgtgc atctctacat gcgagaagcg gccatctacg gggcagtcat
     5581 gctgctcgtg cttggcatgt cgtgctggtt ggtctccgag tgggcgcggc agcttgagcg
     5641 aggcggcttg caattgatcg acatagctgg ccggggcctt gagtcggaag accttacctt
     5701 attttcttat caggattggc agtagtgact gctaacccga taggccaatc tattacggca
     5761 gcgagcgcga aagccgctga cccagcactg tggcgacgtt gggtgacggc aactctcgtg
     5821 gggggctggc ttggctctct gttgagcttg tatgtttact ggaatggcat ctctttttac
     5881 cccctcttct ctgtggttcc cttcataggg agcactctac tcggcctggt cctgggtgtg
     5941 ttccaggggc gagcattaag gaacagcctc ggagcacaag tctggccgca ctgggcactg
     6001 atgacctggc tcggccttat tctgagtatc ggtgttgggg ggattgccgg tccgcagacg
     6061 gaccggataa ttacgagctt aagcgatatc agcaccgaag ctgcgcaatt cgggggagtt
     6121 atactattca gtacactcca ggccctagta gtaggtggcc tgcaatgcat aattctgtgg
     6181 tcgcatacga ggaaagctgc ctggtggccg ttagggcttg cagcgggcct ggttgtgggc
     6241 aattgtattg ctcttgaggt atctatcctg gcaatagcct cgattatagg tccagaccca
     6301 actatgtttg cagtgcccta tatgtttttc atcagcggag tatcgagtgc ttgcattccc
     6361 tggccgttta cagcggtgat aacgggttgg gtattggtaa accttctaaa aaaccctgaa
     6421 aagcagagca caggcattag cagcaactat atcatgacga aattcgagcg tttatacagc
     6481 gagcttgagc gcagatatgg acctgcttca ccagaactaa aacccctcat ggctgcgttc
     6541 ttcgacgcac tcatggctg
//
LOCUS       DASRWZ010000632         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1216747, whole genome shotgun sequence.
ACCESSION   DASRWZ010000632 DASRWZ010000000
VERSION     DASRWZ010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216747"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1768)
                     /locus_tag="VFH60_10060"
     CDS             complement(71..1768)
                     /locus_tag="VFH60_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6314170.1"
                     /translation="MTLSFRPQALLAGVGLALMLAVLLFAPGAGTSTLTRSQPGTYVE
                     AMLGAPRFVNPLLATSDTDLDLTHLVYSALTRVHTKGRLAPDMASRWEVSPDARVYTF
                     TLKSDVRWHDGEFFTADDVLFTIEQLRRSDFPGDPMLAAPWKDVKLEAPTRQTLIFRL
                     PQSNAAFLQHTTLGILPRHLWDDSKAGALATSELNRDPVGSGPWRYARGPETASEASG
                     PGDVFQPTPSALDTGAEGVLLEPNPYGPNAGVPFSRIWFRLYPSFGAALTGFKMGEVH
                     GLGHIAPEQLAEVEAVAGAQAHRQNLARYTMLMLNVRSPLFDRAETRQAFELAIDRRA
                     IVSDSLHGLAEPVASPILPHSWAFDATSRGAGGYDPMKAGQLLDAAGWKLSAAGMRAR
                     DGMTLTVVLAANADVPSNVAVAQQLASSLKGVSVDVRLALVSREALLRDYLGPRAFHM
                     ALVGWEAQGADPDIWSYWHSSQANITGGLNFSGWTNPEADKALDAARATPDLAARAAN
                     YATVQKAFAVDVPAVILYTPTYVYATRAPATGVHLPTSDMLTPAQRFDTLGDWSLQP"
     gene            complement(1866..2078)
                     /gene="secG"
                     /locus_tag="VFH60_10065"
     CDS             complement(1866..2078)
                     /gene="secG"
                     /locus_tag="VFH60_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET6314171.1"
                     /translation="MNIVQVVLALVLIVAILLQTKGSTFSGIFGGGDSSSVYRTRRGF
                     EKRLFQMTIVLSVVFFLVALLNSIVT"
BASE COUNT          423 a          690 c          802 g          395 t
ORIGIN      
        1 acctttgcgc gtcctttgcg ttctttgcgt ctttgcgttt tcgtctttac actcggcact
       61 cggcactcgg ctagggctgt agcgaccaat cgcccagcgt gtcgaagcgt tgggcagggg
      121 tgagcatgtc ggaggtgggc aggtgcacgc ctgtggccgg ggcgcgggtg gcatagacgt
      181 aggtgggcgt gtagaggatg acggcgggca cgtccacagc aaacgctttc tggaccgtgg
      241 cgtagttggc agcgcgtgcg gcaaggtcgg gcgtggcgcg ggcggcatcg agggccttgt
      301 cggcctcggg gttagtccaa ccggagaagt tgaggccgcc ggtgatattg gcttgcgacg
      361 aatgccagta gctccaaatg tcggggtcgg ccccctgcgc ctcccagccc acgagcgcca
      421 tgtggaaggc gcgcggcccg agatagtcgc gcaaaagcgc ctcgcggctc accagagcga
      481 gtcgcacgtc cacgctcacc cccttgagtg agcttgccag ttgctgcgcg acggccacat
      541 tcgagggcac atcggcgttg gcggccagca ccaccgtcag cgtcatcccg tcgcgggcgc
      601 gcattccggc ggcgctcagc ttccagcccg ccgcgtccag caactgccct gctttcatcg
      661 ggtcgtagcc gcccgcccct cgcgaggtgg cgtcgaacgc ccaggaatgc ggcaggatgg
      721 ggctagccac cggctcggcc agaccatgca agctgtcgct cacgattgcc cggcggtcta
      781 tggccagctc gaacgcctgc cgggtctcgg cgcggtcgaa caggggagag cgcacgttta
      841 gcataagcat ggtgtagcgg gcgaggttct gccggtgcgc ctgcgccccc gccaccgctt
      901 ccacctcggc caactgctca ggcgctatgt gacccagccc gtgtacctcg cccatcttga
      961 agccggtgag cgccgccccg aagctcgggt acagccggaa ccagatgcgc gagaagggga
     1021 ctccggcgtt cggcccataa ggattcggct cgagcaacac cccctccgcg ccggtatcca
     1081 gggcggacgg cgtcggctgg aaaacgtcgc cggggccgga cgcctcggaa gcagtctccg
     1141 ggccgcgcgc gtaccgccag gggccggagc cgaccggatc gcggtttagc tccgaggtcg
     1201 ccaatgcccc ggctttcgag tcgtcccaga ggtggcgggg caggatgcca agcgtggtgt
     1261 gttgcaggaa cgcggcgttg gactgtggga ggcggaaaat cagcgtctgc cgggtggggg
     1321 cctccagctt cacgtccttc cagggggccg ccagcatcgg gtcgccgggg aagtcggacc
     1381 tgcgaagctg ctctatcgta aagagcacgt cgtcggcggt aaagaactcg ccatcgtgcc
     1441 agcgcacatc ggacttgagc gtaaaggtat agacgcgcgc gtcggggctg acctcccagc
     1501 ggctcgccat gtcaggcgcg agtctaccct tcgtatggac gcgtgtcagc gcgctgtaga
     1561 cgagatgcgt gaggtcgagg tcggtgtcgg aggtggcaag cagggggttg acgaagcggg
     1621 gagcgcccag catggcctcc acgtaggtgc caggctgcga gcgggtgagc gtggaagtgc
     1681 ccgctcccgg cgcgaacagg agcaccgcga gcatgagggc cagtccaaca ccggccagga
     1741 gcgcctgggg gcgaaacgaa agtgtcatag acaaacagat agccggggcg tggcaaccac
     1801 accaccggcg tattagctat aaggacgtaa gtgggcctac tttgtagcct ggtgggctgc
     1861 taaatttagg taacgatgct gttgagcagg gccaccagga agaagaccac cgacagcaca
     1921 atagtcatct ggaacaggcg cttctcgaac ccacggcgcg tccggtacac cgaactcgaa
     1981 tcgccgccgc cgaagatgcc tgagaaggtg gagcctttgg tctgtagcaa aatagccacg
     2041 atcaagacga gtgcgagcac cacctgtaca atgttcaaag ctgctggtaa tgtcaccgga
     2101 gaatcgctcc ttgcgcgaac acagataatg gtgggcattt agcccataac cgactgatta
     2161 taccattggc ggggcgggaa tgcaaagcgg gtggtcgatg gtcggtcagg gcgcttggcg
     2221 taggtagtgc gtggatgcta ccagaatggg gtgctgggac ccaggtggtc tttgtcattc
     2281 tgagcgtagc gaagaatctc gtcctccatg
//
LOCUS       DASRWZ010000633         3964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1010483, whole genome shotgun sequence.
ACCESSION   DASRWZ010000633 DASRWZ010000000
VERSION     DASRWZ010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010483"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..1862
                     /locus_tag="VFH60_10070"
     CDS             456..1862
                     /locus_tag="VFH60_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HET6314172.1"
                     /translation="MTSISSGKKGVPGERTKYLLDEDRIPTSWYNIQADLHTPAPPSL
                     HPGTGQPIGPADLAPLFPMAIIMQEVSQDRYIDIPEEVREIYKLWRPTPLYRARRLEK
                     ALDTPARIFYKYEGTSPVGSHKANTALAQAYYSKQEGVKRLATETGAGQWGSALSMAT
                     SMFGIECQVYMVKVSYFQKPYRRSLMHAFGARVIPSPSPETNAGRAALERDPESPGSL
                     GLAISEAVEDAAMREDTKYSLGSVLNHVLMHQTVIGQEAQQQMEMADAYPDVVIGCVG
                     GGSNFSGLVFPFVADKLTGKSPDLRVVAVEPAACPSLTKGVFAYDYGDTAMMAPMVKM
                     HTLGHGFIPQSIHAGGLRYHGMAPLVSHLVEGGFIEATAYPQNPVFEAAIQFTRSEGI
                     VPAPESAHAIRAAIDEALRAKEAGESKTILFNLSGHGFFDLAAYDRYLAGDLEDYEYP
                     ADEVQAALAGLPTIASAM"
     gene            complement(2043..2495)
                     /locus_tag="VFH60_10075"
     CDS             complement(2043..2495)
                     /locus_tag="VFH60_10075"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET6314173.1"
                     /translation="MPVSSEEKKQSIRDLYEQGFNARNMDYLDQIMSDQFTDYSSGTP
                     GPLGRDSFKQFLGMYLQAFPDLHFEIEGDIVAEGDYVAWRDVVTGTHQAEFMGIPATG
                     KQIRIVGVHIAQVDETGMATAHWGGVDDLGMLVQLGVIPAPGAQPAMA"
     gene            complement(2719..3867)
                     /locus_tag="VFH60_10080"
     CDS             complement(2719..3867)
                     /locus_tag="VFH60_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314174.1"
                     /translation="MKQCPNPNCILYTRLEELPDAYVKCPGCGGLLESLENQSGRLSS
                     GRLPNRPQASGPSRPPAAGTGTSPRPAQPKLPGLTPPLATDPDPYDPYGVSGPYDPYA
                     PLQPVQSGVAGDPYAAAFADTGQHGASQAAMSQQTPQSAYVSTSSLSTTKWTTASKVA
                     FGVGSVLLLLACGLLGWIFSNRFFPQSRVIASPQATETALALVRPSMNTPIMQPSLTT
                     LAGNFPGPTLVVPTLPVEEPTPLVVVPTSAPPTEVPLVVPPTQPSGQAAPPAQQEPVG
                     GILEAHTMTGQGNPTVQFGPNEPFNLAVRATFGPGNVTNVLTRWYGPDGAQVYAMQKD
                     YTQPGAYYVAFSVRKDTPWLPGDYRVDIYTNHSPTPSYSVFFSVTSNE"
BASE COUNT          807 a         1221 c         1232 g          704 t
ORIGIN      
        1 ggcaccaatc accgcaacaa cggcaggccc gctatcgtaa ccacctgagc gatacagcgc
       61 ccacggaagt gcttgcggag gaatggtaga cattactaat accagccagg cgataagtag
      121 cacgacaaag cataccgtga agcaacccgg tttcgaacgc tttgctgttt cgtcaggtgc
      181 agtatccgcc atagttacag cgcgcaagaa tctattgaca cccccaactc ccagcatcta
      241 tactaacaca accctccgat accagaaagc gggaacaggg cgaagacatc cgagagcggg
      301 gttacagggg tggcaccacg ggataacccc gttccttcat ggaacgcggg ttgttgtatt
      361 ttaagtgccg agtgccgagt gccgagtgcc gagtgccgag tgcgcggtaa gagtatagcg
      421 accatcgacc actagcaagc gaagggagca acaagatgac gagcatatcc agtggcaaga
      481 agggagtgcc gggcgagcgg accaagtacc tgctcgatga ggacaggata ccgacctcct
      541 ggtacaacat acaggccgac ctgcacaccc ccgcgccccc gtccctgcac cccggcaccg
      601 gccagccgat tggccccgcc gacctcgcac cgctcttccc gatggcgatt atcatgcagg
      661 aggtcagcca ggaccgctac atcgacatcc ctgaggaggt gcgcgaaatt tacaagctgt
      721 ggcggcccac ccccctatac cgcgcgcgca ggctggagaa ggcgctcgat acccccgctc
      781 gcatcttcta caagtacgag ggcaccagcc ccgtgggcag ccacaaggcc aataccgcgc
      841 tcgcccaggc gtactatagc aagcaggaag gcgtgaagcg actcgccacc gaaacgggag
      901 cgggccagtg gggtagcgcc ctctcgatgg ctaccagcat gttcgggata gaatgccagg
      961 tgtacatggt gaaggtatcc tacttccaga agccctaccg ccgctcgctc atgcacgcgt
     1021 tcggcgcgcg ggtcatcccc agccccagcc cagagaccaa cgcgggccgc gccgccctgg
     1081 agcgagaccc cgaatccccc ggcagcctgg gcctggctat ctcggaggcg gtggaggacg
     1141 ccgccatgcg cgaggacacc aagtactcgc ttggcagcgt gctcaatcac gtgttgatgc
     1201 accagacggt gataggccag gaggcgcagc aacagatgga gatggccgac gcctaccccg
     1261 acgtggtgat aggctgcgtg ggcgggggca gcaacttctc cgggctggtc ttcccgttcg
     1321 tggccgacaa actcaccggc aagtcgcccg acctgcgcgt ggtagccgtg gagcctgccg
     1381 cctgcccctc gctcaccaag ggcgtattcg cctacgacta cggcgacacc gctatgatgg
     1441 ccccgatggt caagatgcac acgctggggc acggcttcat accccagtcg atccacgcgg
     1501 gcggcttgcg ctaccacggc atggccccgc tcgtcagcca cctggtggag ggcggcttca
     1561 tcgaggccac cgcctacccg cagaaccccg tgttcgaggc ggccatccag ttcacccgca
     1621 gcgaaggcat cgtgcccgcc cccgagtcgg cccacgccat acgcgccgcg attgacgagg
     1681 cactgcgcgc caaagaagcg ggcgagagca agacgatcct gttcaacctg agcggccacg
     1741 gcttcttcga cctcgccgcc tacgaccgct acctggcagg cgacctagaa gactacgagt
     1801 accccgccga cgaagtccag gccgccctcg cgggcctccc caccatcgca agcgcgatgt
     1861 agaaacggag ggacgaaaac gcaaagacgc aaaggacgca aagaacgcgc aaagagaaga
     1921 attgtttaga ggatgcgaaa aggaccagag tacaactata gtaccctggt ccttttgctt
     1981 agaacaccat tatcttaatc tctgcgtgtc ctttgcgtcc tttgcgtctt tgcgttttcg
     2041 tcttatgcca ttgcgggctg ggcaccgggc gcgggtatca cgccgagttg taccagcata
     2101 cccaggtcgt ctacgccgcc ccagtgggcc gtcgccatac cggtctcgtc aacctgggcg
     2161 atgtggaccc cgacgatacg tatctgcttg ccggtggccg ggatacccat gaactcggcc
     2221 tggtgggtgc cggtcaccac gtcccgccag gcaacgtaat cgccttccgc gacaatgtct
     2281 ccctcgatct cgaagtgcag gtcggggaag gcttgcaaat acatgcctag gaactgcttg
     2341 aagctgtccc ggcccagggg tccaggcgta ccgctcgagt agtcggtaaa ttggtcgctc
     2401 atgatctggt ccaggtagtc catgttgcga gcgttgaacc cctgctcgta caggtctctg
     2461 attgactgct tcttttcctc agacgaaacg ggcattatat tttcctccat catgcaaata
     2521 tattctttac gtgtatggcc gcaaccggta gtcgctacac acaggaatat agcggcggca
     2581 gatgctcagt tgccagcccg aaagatgctc aattggtgct caatagagga agggagtaac
     2641 gagtaataac gagtaacacg taacaagtgg ctgtaggaca cacagtccct acagccactt
     2701 gttacgtgtt actcgttatt actcgttact cgtcacgctg aagaagacgc tatacgaggg
     2761 tgtgggggag tggttggtgt agatgtccac acggtagtcg ccgggcagcc agggggtgtc
     2821 tttgcgtacc gagaaggcca cgtagtacgc gcccggctgg gtgtagtcct tctgcatagc
     2881 gtatacctgc gcgccgtcag gcccatacca gcgggtaagc acgttggtga cgttgccggg
     2941 gccgaacgtg gcccgcaccg ccaggttgaa cggctcgttg gggccgaatt gtacggtcgg
     3001 gttgccctgc cctgtcatcg tgtgcgcctc gaggatgccc ccaacaggct cctgctgggc
     3061 gggaggggcc gcctgacctg aaggctgcgt gggcggcacg accagtggca cctctgtggg
     3121 cggggcggac gtgggcacta ccactagcgg cgtaggctcc tccacgggga gagtgggcac
     3181 caccagcgtg gggccgggga agttacccgc caacgtggta agcgagggct gcatgatggg
     3241 cgtgttcatc gagggccgga cgagagcaag ggctgtctcg gtggcctgcg ggctggctat
     3301 cacccgcgat tgagggaaga agcggttgct gaatatccac cccagcagcc cgcaagccag
     3361 cagcagtagc acgctgccca ccccaaaggc taccttgctc gccgtggtcc attttgtcgt
     3421 ggacagggag gaggtactca catacgcaga ctgcggcgtc tgttgagaca tggcggcttg
     3481 cgaggcccca tgctggcccg tgtcggcaaa tgcggccgcg taagggtcgc ccgccactcc
     3541 cgactgcacc ggctgaaggg gcgcgtacgg atcgtacggg cccgacacgc cgtagggatc
     3601 gtagggatcg gggtcggtgg cgagtggggg agtgaggccg ggcagcttcg gctgtgccgg
     3661 cctgggcgac gtgcctgtgc cggcagccgg tgggcgggaa ggtccgcttg cctgcggccg
     3721 gttcgggagc cgccccgaac tcaaccgccc cgattggttc tccaggcttt ccaaaaggcc
     3781 gccgcaccca gggcacttca cgtaagcgtc aggcagctct tccaggcgcg tgtagagtat
     3841 gcagttggga ttgggacatt gcttcattgt gcgtttggta ccacaggttg cgggccatgg
     3901 gccagagtta ccttagcctc aatcggggcc tgatgttata atctagcaca ctatgcgtct
     3961 cagg
//
LOCUS       DASRWZ010000634         2331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_805228, whole genome shotgun sequence.
ACCESSION   DASRWZ010000634 DASRWZ010000000
VERSION     DASRWZ010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805228"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFH60_10085"
     CDS             complement(<1..622)
                     /locus_tag="VFH60_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6314175.1"
                     /translation="MTTQPSNSAERDGTAVAGRRILVADDDNAIRQLLKTFLEDSDYT
                     ISEATTGQEALDGLKSGNYDLVLLDMRLPGMTGIDVLKQLREKTGEVPVILITAYGSP
                     NIAIQASSLGAYSYITKPFELDDVLHTINLYFERRQLKEEVRSLRSQIEPRDPSERII
                     GSSTAMHDIYQVIGRAAPTEATVLISGETGTGKELVANVIHTYSSYR"
     gene            complement(994..>2331)
                     /locus_tag="VFH60_10090"
     CDS             complement(994..>2331)
                     /locus_tag="VFH60_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314176.1"
                     /translation="GGYKLLSLVAALALLATQANPTRTSAATQAQPACGAWSIVPTSG
                     TALDLGRFKGVAATSYSNVWAIGSTSDQNDLLARWDGTDWSLEPHPQPGSFFNDFQGI
                     ATSSDGDAWIVGAYSSSRASRPSMAETSTLTQRLQGGRWKLVPSPNHPNKPQSYLNSI
                     APIGPDDVWAVGNGGGEDIVLRWNGAEWTFTPVPVAAQGGYLFDVAGTSPGDVWAVGV
                     YTTDKFTQAARILRWNGADWQLMPSPDLPSDEYNLQGVAALTPNDAWAVGSRTENGRT
                     LTLIEHWDGSAWTVVPSPNTTRTENTLHRVTATAPNDGWAVGRTGTGGDAQPLMLHFD
                     GTAWANVTLPRVNARFSELVSIATVPGSTEIWAVGAYAGANSREHTLAMRYRPCADGG
                     GTPGEGVPGGGIPGMPATGAEGNPPLAAQAVATLLHDSVAALLALIRQLPGLF"
BASE COUNT          381 a          666 c          797 g          487 t
ORIGIN      
        1 ggcgatagct ggagtaggtg tggattacgt tcgccacaag ctctttgccg gtgcccgttt
       61 cgccgctaat cagcacggtg gcctcggtgg gtgcagcgcg acctatgacc tggtagatgt
      121 catgcatggc cgtagacgag ccgatgatgc gctccgaggg gtcgcgcggc tctatctgcg
      181 agcggaggct gcgcacttct tccttgagct gccgccgctc gaagtatagg tttatcgtgt
      241 gcagcacgtc gtcaagctcg aagggcttgg tgatgtagct gtacgcgccg agggaggacg
      301 cctggatcgc gatgttgggc gagccgtagg ctgttatcag gatgacgggc acctcgcccg
      361 tcttctcccg cagttgtttg agcacgtcaa tcccggtcat gccgggcagg cgcatgtcga
      421 ggagcaccag gtcgtaattg cctgatttga ggccgtctag ggcctcctgg ccggtggtgg
      481 cttcggaaat cgtatagtcg ctgtcttcga ggaaagtctt gagtagctgc cgtatagcgt
      541 tatcgtcatc ggccaccagg atgcggcgtc cggctacggc agtgccgtct ctctcggcgc
      601 tgttgctggg ctgtgtcgtc acagaggagt ctacctcacc attcgaaccg ttcttcaaat
      661 cttcaacgtc aagtacgctc gttgtacgag ccgaagcacc attgccgctt ttcttcgtca
      721 tcaaggctcc ctttgctaga agaccgcgta gcgggtgcct ccagcagctt aatcgccgcc
      781 gggcagaccg aaacacatgc gcggaatact agtgctacga ttacctctcc attatagagc
      841 agggtggcag ggccgtcaat tgaatatagt acaaaagtgc tatctattga caaaccttta
      901 ctccccttta agtaacgttc cacttcataa aaagatagct cgttatctcc tacgcacggg
      961 cggcccggat ggttcatcag gcggcatatt gtcttagaac aagcccggaa gctgccttat
     1021 cagagcaagc aaagcggcaa cagaatcgtg caggagcgtg gcgacagcct gggccgcaag
     1081 tggcgggttg ccttccgccc cggtggcggg catgccgggt atgccgccac ccggaacgcc
     1141 ttctccgggg gtgcccccac cgtccgcgca cggcctgtag cgcatggcga gggtgtgctc
     1201 ccggctgtta gcgccggcgt aggcccctac cgcccatatt tccgtgctgc cgggcacggt
     1261 tgcgatggat acgagttcgg agaagcgggc gttgacgcgt ggcagggtta cgttggccca
     1321 ggcggtgcca tcaaagtgca gcattagcgg ttgggcgtcg ccgccggtgc cggtgcggcc
     1381 taccgcccaa ccgtcgttgg gggcggtggc cgtgacgcgg tgcagggtgt tctccgtccg
     1441 cgtggtgtta gggctgggca cgacggtcca ggcgctgccg tcccagtgtt ctatgagggt
     1501 gagggtgcgg ccgttctcgg ttcggctccc gaccgcccag gcgtcgttag gggtgagggc
     1561 cgctacgcct tgcaggttgt attcgtcgga ggggaggtcg gggcttggca taagctgcca
     1621 gtccgcgccg ttccagcgga gtatgcgggc ggcctgggtg aatttgtcgg tggtgtatac
     1681 gcccaccgcc catacgtcgc cggggctggt gcctgctacg tcgaacaggt agccgccctg
     1741 cgccgcgacg ggtacgggcg tgaaggtcca ctctgcgccg ttccaacgga gcacgatatc
     1801 ctcgccgccc ccgttgccca ccgcccaaac gtcgtcgggg ccgatgggag ctatcgagtt
     1861 gagatagctc tggggcttat tcggatggtt ggggctgggt accagcttcc acctgccgcc
     1921 ctgtagacgc tgggtaaggg tggaggtctc ggccatgctg gggcggctgg cacgcgagct
     1981 tgagtacgcg cccacgatcc aggcgtcccc atcggaggag gtggctatgc cctggaagtc
     2041 gttgaagaac gagccgggct gtgggtgagg ctcgaggctc cagtcggtgc cgtcccaacg
     2101 cgctagcagg tcattttggt cggaggtgct gccgatggcc cacacgttgg agtaggaggt
     2161 ggctgccacg cccttgaagc gtcccaggtc gagggctgtt cctgaggtgg ggacgatgga
     2221 ccacgctcca caggcgggtt gggcttgcgt ggcggcgctt gtacgtgtgg ggttggcctg
     2281 ggtagcgagc agggcgaggg cggcaactag cgacagcagc ttatatccgc c
//
LOCUS       DASRWZ010000635         3977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1285573, whole genome shotgun sequence.
ACCESSION   DASRWZ010000635 DASRWZ010000000
VERSION     DASRWZ010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285573"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1309
                     /locus_tag="VFH60_10095"
     CDS             137..1309
                     /locus_tag="VFH60_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6314177.1"
                     /translation="MPGIVVEPGSAEEVSEVLAWANGEGLKAVVCGARTKLDRGAAPA
                     ACDVLLDLGKLNRVTEHASGDLTVTVQAGVRLEELQRQLAEAGQFLAVDPPVPGTVGG
                     LVATADTGPRRLRYGGVRDLILGATFVRADGVIAKAGGKVVKNVAGYDLPKLLTGSLG
                     TLAVVVEATFRLYPLPAASATVLIDVAPAELRRQAGTVLQSTLVPTSLDYFSDAAGSS
                     LLAVRFESTTASSHAQAEVAARLLGGSSRIVTGAEETALWAGFDAVIDTTDNDVLARL
                     VATPSDLPGLVAQALRGASAAGAQLTVRAHVGHGHALLRWREANSDTALSLLRGLRYD
                     AESQGHNLVIWRAPQEVRAGIDVWGSVGEGLGLMRLVKQQFDPKSTLNPGRFVGGI"
     gene            1491..2837
                     /locus_tag="VFH60_10100"
     CDS             1491..2837
                     /locus_tag="VFH60_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET6314178.1"
                     /translation="MEAVRGAGGSSLPLRLFPAFDAHNPPSEELIDDCVHCGFCLPTC
                     PTYLLWGEEMDSPRGRIHLMKAGNEGRLGMTDTFVRHFDQCLGCMACVTACPSGVQYN
                     KLIESTRSQVERRYRRNPADRLFRRLVFTLFPYPSRLRLLAPFLLLYRASGLQGIVEL
                     TGLLDRLPPRLRSMHDLMPRMTLKRLRMRVPGRVQPGGRARARVGLLTGCVQQVFFPQ
                     VHAATVRVLASEGCEVIAPAGQACCGALALHAGYDEQAQQFARKLIDVFERERLDYIA
                     VNAAGCGSTMKEYGHLLKDDPEYAGRAARFAAGVRDITELLVELGPAATRHPLRAKVA
                     YHDACHLAHAQGIRREPRELLQGIPGVELVPVTESDICCGSAGIYNLVEPQPAGELGE
                     RKARHILAMGARIVASANPGCTLQIEAHTGRLGAGLKVLHPVEILDRSLHPRRKDR"
     gene            2838..>3977
                     /locus_tag="VFH60_10105"
     CDS             2838..>3977
                     /locus_tag="VFH60_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET6314179.1"
                     /translation="MLEATDRITVPAPASPPKRLLMGPGPSEVDPEVLRSLLMPPLGH
                     LDPALLQIMQELEDMLRAMFATRNRVTLAISGTGTAGMEAALANTVEPGAHVVVGVIG
                     YFGDRMCQIAERLGGVVTRVESEWGRPLYAEAMWEAIQRVRPRVVCAVHAETSTGVRQ
                     DLTDIAAAAHEVDALFVVDAVTSLGGQPLLVDEWGIDVCYSGSQKCLGAPSGLSPFTI
                     NDRALERAAQRTRPVPSFYLDAALLHRYWDERQYHHTISAPLIYSLHTALRLLHTEGL
                     ENRWERHRRSHLAYRAGVEAMGFGFLPDEEWSLWPLNAILLPEGLDELELRAHLLNRH
                     GIEVGGGLGPLKGRLLRVGLMGYGAQEGFVLHLLGALEETLRSFGL"
BASE COUNT          670 a         1256 c         1374 g          677 t
ORIGIN      
        1 tgataaatta gccaattgac taatcaaatt aaaaatctgc gacattttgc ttatcatgag
       61 cacaaattca gacacacata taagtatccg tgcagataac agggccgcta cacctcagga
      121 tgaggtgctt ggcattatgc ccggcatcgt agttgagcct ggtagcgcgg aagaggtgtc
      181 tgaggtgctg gcctgggcca acggcgaggg attgaaggct gtagtctgcg gcgcgcgcac
      241 caagctcgac aggggagcgg caccggcggc ttgtgatgta ttgctcgacc tgggtaagct
      301 caaccgggtc acggaacatg cctccggcga cctcacagtg acggtgcagg ccggggtgcg
      361 gttggaggag ttgcagcgac aacttgccga ggcggggcaa ttccttgccg tcgatccccc
      421 ggtgcccggc acggtgggag ggctagtagc cacggcggat accgggccga gacgcctccg
      481 ctacgggggc gtgcgcgacc tcatcctggg cgcgactttc gtgcgggccg acggggtgat
      541 agccaaagcc ggaggcaagg tggtcaaaaa cgtggcaggg tacgacctgc cgaagctgct
      601 gaccggctcg ctgggcaccc tggcggtggt tgtggaagcc accttccggc tttacccgct
      661 gcccgctgcc tcggctactg tgctgattga tgtcgccccc gccgaactgc gccgccaggc
      721 cggcacggtc ctgcaatcga ccctggtgcc cacttctctc gactactttt ccgacgcggc
      781 aggttcgtct ttgctggccg tccgcttcga gagtactacc gcatcctcgc acgcacaggc
      841 cgaggtggcc gcccgcctcc tgggtggcag cagccgtatc gtgacgggtg cggaggagac
      901 agccttgtgg gcagggttcg acgctgtaat cgataccacc gataatgacg tactagcccg
      961 cctggtagcc accccgagcg accttccggg gctggtggcc caggcgttgc gtggggcaag
     1021 cgcggcaggt gctcagctaa ccgtgcgcgc ccacgtggga cacgggcatg cgctcctcag
     1081 gtggcgtgag gcaaacagcg acacggccct ttccctgctt cgaggcctgc ggtacgatgc
     1141 tgaatcccag ggccacaatc ttgtcatctg gcgggcaccg caggaggtgc gtgcgggcat
     1201 cgacgtatgg ggcagcgtgg gcgaggggct ggggctaatg cgcctggtga agcagcagtt
     1261 cgaccccaaa tcgacgctca accccggccg cttcgtgggc ggcatatagc ggaagcgagg
     1321 cagcggtgga acactcacgc gacagggagc aactggagga agcgcgcccc ggctacgagc
     1381 gagcgaggtt cggggtgccc gcgcacgtgc aggcagctgc cgaggtggca ggcgaagtct
     1441 cggtgcgctc cggcccgcag gatgtaggcg ctgccgctga cggctccggg gtggaggcgg
     1501 tgcgcggcgc gggtgggagc agcctgcctt tgcggctgtt cccggcgttc gacgctcata
     1561 acccgccttc cgaggagttg attgacgatt gcgtgcactg cggcttctgc ctgcctacct
     1621 gccccacgta cctgctttgg ggcgaggaaa tggactcgcc gcgcggccgt attcacctca
     1681 tgaaagccgg caacgaaggc aggctgggca tgaccgatac cttcgtacgc cacttcgacc
     1741 agtgcctcgg ctgcatggcc tgcgtgaccg cctgcccctc aggggtgcag tacaacaagc
     1801 tgatagagtc cacccgcagt caggtggagc gtcgctacag gcgcaacccg gcagacaggc
     1861 tgttccgcag gctggtgttc acgctcttcc cctacccctc gcgcctccgg ttgctggccc
     1921 cgttcctgct gctgtaccgg gcatcggggt tgcagggcat cgtggagctc accggcttgc
     1981 tcgacaggct cccaccgcgc cttcgcagca tgcacgactt gatgccgcgt atgacgttga
     2041 agcgcctgcg gatgcgcgtg ccgggacgcg ttcagccggg aggtcgggcg agggcgcgcg
     2101 tggggctgct gactgggtgc gtgcagcagg tattcttccc tcaggtgcac gcggccacgg
     2161 tgcgcgtgct ggcgagcgag gggtgcgagg tgatagctcc agcggggcag gcatgctgcg
     2221 gcgcgctggc cctgcatgcc gggtacgacg agcaggcgca gcaattcgcc cgcaagctga
     2281 ttgacgtgtt cgagcgggag cggctggatt acatcgccgt gaacgccgcc gggtgtggct
     2341 cgaccatgaa ggagtacggc cacctcttga aggacgaccc ggaatatgcc gggcgtgccg
     2401 ccagatttgc agccggagtg cgcgacatta cggaattgct ggtcgagcta gggccggccg
     2461 ctacgcgcca cccgctacgc gcgaaggtag cttaccacga cgcctgccac ctggcgcacg
     2521 cccagggcat ccgccgcgag cctcgcgagc ttttgcaggg catccccggt gtggagcttg
     2581 tgcccgtgac ggagtccgac atatgctgtg gtagcgcggg gatttacaac ctggtggaac
     2641 cacaacccgc cggtgagttg ggagagcgca aggcccggca cattctcgcg atgggtgcca
     2701 ggatagtggc gagcgccaac cccggctgca ccttgcagat agaggcccac accggccgcc
     2761 tgggggccgg cttgaaagtg ctgcacccgg tggaaatact tgacaggtcg ctgcacccgc
     2821 gacgaaagga ccgatagatg cttgaagcca ccgatagaat cacagtcccc gcgcccgcca
     2881 gcccgcccaa gcggctgctg atggggccgg ggccgagcga agtagacccg gaggtgttac
     2941 gctcgctgct catgcctccg ctggggcacc tcgatcccgc gctgctccaa attatgcagg
     3001 agcttgaaga catgctccgg gcgatgttcg ctacccgcaa ccgcgtcact ctcgccattt
     3061 caggcaccgg cacggcgggc atggaagcgg ccctggcgaa cacggtcgag cctggggcgc
     3121 acgtcgtagt gggggtgatt ggctactttg gcgaccgcat gtgccagatt gcggagcggc
     3181 tgggcggcgt agtcacgcgg gtagagagtg agtggggcag gccgctgtac gccgaggcga
     3241 tgtgggaggc catccagcgc gtcaggccgc gcgtagtgtg cgccgtgcac gccgagacct
     3301 ccacgggcgt gcgccaggac ctcaccgaca tagcggcggc cgcgcacgag gtggacgcac
     3361 ttttcgtggt ggacgcggtg acctcgctgg gcgggcagcc gctgctggtg gacgagtggg
     3421 gcattgacgt gtgctacagc ggcagccaga agtgtctcgg cgcgccctcc ggcctgtcgc
     3481 ccttcacgat caacgaccgc gccctggaac gtgccgccca acggacgcgt cccgtgccca
     3541 gtttctacct ggatgccgcg ctcttgcatc gctactggga cgagcgccag taccaccaca
     3601 ccatatccgc gccccttatc tactcgctgc acaccgccct gcgcctgctg cacaccgagg
     3661 gcctggaaaa caggtgggag cggcaccgac gcagccacct ggcctaccgc gcgggcgtag
     3721 aggctatggg ctttggtttc ctgccagacg aggaatggag cctgtggccc ctgaacgcca
     3781 tcctgctccc cgaggggctg gacgagcttg agctgcgcgc tcacctgctc aaccggcacg
     3841 gcatcgaagt cggcggtggc ctgggaccgc taaagggcag gctgctacgc gtggggctga
     3901 tgggctacgg cgcgcaagag ggcttcgtcc ttcacctgct cggcgccctg gaagagaccc
     3961 tacgctcctt cggcctc
//
LOCUS       DASRWZ010000636         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_668360, whole genome shotgun sequence.
ACCESSION   DASRWZ010000636 DASRWZ010000000
VERSION     DASRWZ010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668360"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1855)
                     /gene="glmS"
                     /locus_tag="VFH60_10110"
     CDS             complement(59..1855)
                     /gene="glmS"
                     /locus_tag="VFH60_10110"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET6314180.1"
                     /translation="MCGIFGYTGERIDAANLVFSGLKTLEYRGYDSWGIAVGTDDGVQ
                     TVRDVGKLVAAPGGMPHSGLALGHTRWATTGAVTQANAHPHRDCTGRFALVHNGIIEN
                     YALLREGLKERGHRFQSETDSEVVAHMLEEEYDTVGQLPVDVESRLAEALRRVSHKLD
                     GLNALAALDVRTGELAAVKNGSPLVVGIGDGATYLASDQAALLAHTDKIVFVRDGQAV
                     ALWPDHASLIDSLTGTSIPLEVEQVRWRPEQAQLNGYNHFLEKEIWEQPKLLREIASG
                     GMEQAIGLASFLAESRGIYFVGCGTAYHAALIGSYLMSTQARRLTTVSYPHEFARYAP
                     LVKGYDAVLAFSQSGETIDVLDAVREARARGAAIGAMTNVPGSTLTREASFKLMLGAG
                     PERSVLSTKTFTAKVAYMMLAAGVIAGEPEAAKATLLEAASDIEALKDGTDGRLDAIR
                     RVAHCVAPHDHMFLLGRGLGHAMSLEAALKIKEVSYIHAEGFAAGELKHGVIALVEPG
                     TPVLVLAPDGADEAHMLTAAGEVKARGAQVIGIGPRNHPVFDFHVPVTAEGDAFYLAA
                     TAVTQRLAYELALLRGTDPDKPRNLAKSVTVR"
     gene            complement(2395..>3455)
                     /locus_tag="VFH60_10115"
     CDS             complement(2395..>3455)
                     /locus_tag="VFH60_10115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6314181.1"
                     /translation="SGYLVAAFTSGPLVGRLGTRLFLTLGVGAFITGGVSIGLALPFF
                     MLLAFYLLFFFGFGVLDAGLNTYIAGLPNNTAVLNNLHACYGLGALIGPIVAQTFLDL
                     RFGWNAVYLAWAGVALLVLAGVWFAFRGAGRVRHEEGPGGGGNVLAATLRLRVVWIVA
                     IFLLLYVGGEVSLGNWSYSLLTEDRAVDPTPASWFVSGYWMGLTVGRLVLGRLAKRLG
                     DRNLIQLCLAGVMAGLLLVWLPPWPLAAALGLWLTGFSLGPIFPTVIAVLSRMVAPRV
                     LPSAIGFVASAGSAGAAFFPWIAGNLAEGLGLWTLLPYVIGLSVLMLGCWWLLQAQSK
                     AGAPQSEVEQAELGVSRT"
BASE COUNT          581 a         1146 c         1111 g          617 t
ORIGIN      
        1 cgtacttggc gcgctcttgg cgtccttggc gtcttggcgg tttcgtctct ttctgtctct
       61 accggaccgt gacgctcttg gcgaggttgc ggggcttgtc ggggtcggtg ccgcgcagga
      121 gggccagttc gtaggccagg cgctgcgtga cggcggtggc agccaggtag aaggcgtcgc
      181 cctcggccgt gacgggtacg tggaagtcga acacggggtg gttgcggggg ccgatgccta
      241 tcacctgcgc gcctctcgcc ttgacctcgc ccgccgccgt gagcatgtgc gcttcgtccg
      301 ccccgtcggg tgccagcacc agcacgggag tgcccggctc caccagggcg attacgccgt
      361 gcttcaactc cccggctgcg aagccctccg cgtggatgta cgacacctcc ttgatcttga
      421 gcgcggcctc caatgacatg gcgtgcccca ggccccgccc aagcaggaac atgtggtcgt
      481 gcggggcgac gcagtgggct accctgcgta tcgcgtctag cctgccgtcg gtgccgtcct
      541 tcagggcctc gatgtccgac gcggcctcca acagagtggc cttcgcggcc tctggctcgc
      601 ccgctattac gcccgctgcc agcatcatgt aagccacctt ggcggtgaag gtcttggtgg
      661 acagcacgct cctctccggc cccgcgccca gcatcagctt gaagctggcc tcgcgggtga
      721 gggtcgagcc gggcacgttg gtcatcgcgc ctatagcggc cccacgggcg cgggcctcgc
      781 gtaccgcgtc cagcacgtca atcgtctcgc cgctctgcga gaaagccagc acggcgtcgt
      841 agcccttgac gagcggggcg tacctggcga actcgtgggg gtaagacacc gtggtgaggc
      901 ggcgggcctg ggttgacatg aggtagctgc caatcaaggc ggcgtggtag gcggtgccac
      961 agcccacgaa gtagatgcca cgggactcgg ccaggaagct agccagcccg attgcctgct
     1021 ccatgccccc gctcgcgatc tctcgcagca gcttcggttg ctcccatatc tccttttcga
     1081 ggaagtggtt gtagccgttt agctgcgcct gctccggccg ccagcgcact tgctcgacct
     1141 ccagggggat ggacgtaccg gtgaggctgt ctatgagcga ggcgtggtcg ggccagagag
     1201 ccacggcctg cccgtcgcgc acgaacacga tcttgtcggt gtgcgccagc agcgcggcct
     1261 ggtcggacgc caggtaagta gcgccgtctc caatgccgac tacgagcggc gagccgttct
     1321 tcacagcggc aagctccccg gtgcgaacgt cgagggccgc cagggcgttg aggccgtcca
     1381 gcttgtggct aacccggcgc agcgcctcgg ccagcctgct ctccacgtcc acaggcagtt
     1441 gccctaccgt gtcgtactcc tcttccagca tgtgggccac cacttccgag tcggtctccg
     1501 attggaagcg atgcccccgc tcctttagtc cctcgcgcag gagggcgtag ttctcgatga
     1561 tgccgttgtg gacgagggcg aagcgcccgg tgcagtcgcg gtgggggtgg gcgttggcct
     1621 gggtgaccgc gccggtggtg gcccagcgcg tgtgccccag cgccaggccg gagtggggca
     1681 tgccgcctgg ggccgctacc agcttgccga catcgcgcac cgtttgcacc ccgtcgtcgg
     1741 tgcccacggc aatgccccaa gagtcgtacc cgcgatactc gagggtcttc agcccgctaa
     1801 aaaccaggtt ggcggcgtct atgcgctcgc ccgtataacc aaaaataccg cacatgtgaa
     1861 tttatctcct tcggggacga tgggagtatc gcaattacga attacgaatt acgaattacg
     1921 cgttactaca cgcagcacac ggtagggact acgcacgcag cccgtcgtcc gttggtgtgg
     1981 gttattgggt tgatgaagaa gggtaggtaa gaagtgctta ctggtgtaag gttggaagga
     2041 gatagcacga gcaggtgccc ggagcgaagg tgctccgcca cgcgccgccg gttggtgtcc
     2101 tgggggtcgc cctccagttt tgtccggcgc taacgaccgt ggtagccggg aagccatccg
     2161 ccgaatattt ctcttggcct ccacctcgtc aactcaccgc ctcacgatga gtcctggcgc
     2221 tactaattca tatggctcga tgttgtgctg ctcgccgtct cggtcttcct ggctgtacct
     2281 cctggcgtcg atgtgtgctg tttgggcaca tacgtatgat agcacgttgg cctggggatt
     2341 acaattactc ctttgtagtg agtatggtta cccgttagag gtattagtga gtgattaggt
     2401 cctagacact cctaactctg cctgttcgac ttcgctctgt ggcgctccgg ctttagactg
     2461 cgcttgcaac aaccaccagc accccagcat cagcaccgac agccctatca cataaggcaa
     2521 cagcgtccaa agccccagcc cttccgccag gttgcccgct atccagggga agaaggccgc
     2581 acccgcgctg cccgcgctag ccacgaagcc tatcgcgctg gggagcacgc ggggcgctac
     2641 catgcgcgag aggaccgcta tgaccgtggg gaagataggg ccgaggctga agccggtcag
     2701 ccacaggccc agggccgccg cgaggggcca ggggggcagc cacaccagca gcagccccgc
     2761 catgaccccg gccaggcaaa gctggatgag gttgcggtcg cccagccgct tagccagcct
     2821 gccgagcacc aggcggccga ccgtgaggcc catccaatag ccgctgacga accagctcgc
     2881 cggggtgggg tcaacggccc ggtcctcggt gagcaggctg tagctccagt tgcccaggct
     2941 cacctcgccg cccacgtaga gcagcaggaa tatggcgact atccacacca cccgcagccg
     3001 tagcgtggcc gccagcacgt tgcctccccc gccggggcct tcttcgtgcc gcactctgcc
     3061 cgcgccccgg aacgcgaacc acacgcccgc caataccagc aaggccacgc ccgcccaggc
     3121 caggtagacc gcgttccagc cgaatctcag gtcgaggaag gtctgcgcca ctatggggcc
     3181 tatcagcgcg cccagcccgt agcaggcgtg caggttgttg agcaccgcgg tgttgttcgg
     3241 caggcccgcg atgtaggtgt tcagccccgc gtcgagcacg ccgaagccaa agaagaagag
     3301 gaggtagaag gccaacagca tgaagaaggg cagcgccagc ccgatgctca ccccgcctgt
     3361 tatgaacgcg cccactccca gcgtgaggaa cagccgcgtg cccagcctcc ctaccaacgg
     3421 cccgctggtg aaggccgcca cgaggtaccc cgacg
//
LOCUS       DASRWZ010000637         1536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1285618, whole genome shotgun sequence.
ACCESSION   DASRWZ010000637 DASRWZ010000000
VERSION     DASRWZ010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285618"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1536)
                     /locus_tag="VFH60_10120"
     CDS             complement(<1..>1536)
                     /locus_tag="VFH60_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314182.1"
                     /translation="VVPTNAQGLPITPPPMPTFPSPTPTPTYVYKDQGYRAVGGVAWS
                     PDGALLASFDGHYIRVWDPATGGQVRRMRSASEVVKIAWSPDGSLLASLDGETAKSPS
                     IFTMYVNQQPAFYDGTLRLWDPTAGKELQSVWPGQVSDFEWSPEGNKIAVRTGTSASI
                     WSADGTIATPNESLTPTSTAQPGSTATAGMSTGAVCGAWSVVPVPDISAQESELRSVA
                     ALGNEAWAVGYSSNSKRDTATGQMRDARTFIVHWSDTAGAWEHVPSPNFGSGDNVLNG
                     VAAISNSETNAIEVWAVGYYAGPNSERRALALRWDGTTWRQTPIPGVGEIDSALNAVS
                     GSAGNDVWAVGTLGGRHTLTLHWDGTRWSRVPSPSPGIDLNELTSVYAKSPGEVWATG
                     KYLSRGMPIGAPQGDPLVLRWDGSRWRPVPVQFGGTYVNAVSIAPDGEGWLVGGYAGE
                     GGGSGALSRLRDGRGEIVPSPQIGLSTNAQGQSNVPDSNVNGVAILTSNDAWAVGHFS
                     TYGP"
BASE COUNT          250 a          508 c          489 g          289 t
ORIGIN      
        1 gggccatagg tactgaagtg cccaaccgcc caggcgtcat tgctcgtcaa aatcgctacg
       61 ccgttgacgt tactgtcggg cacgttcgat tgaccctgcg cattcgtgct caatcctatt
      121 tgcggggaag gcacgatctc cccgcgccca tctctcaggc gcgagagtgc gccactgcct
      181 cctccctcac ccgcgtagcc gccaaccagc cagccttccc cgtcgggcgc tatgctcacg
      241 gcgttcacgt aagtgccgcc gaactgcacc ggcaccggcc tccagcggga accatcccaa
      301 cgcaacacca gcgggtctcc ctgcggagcg cctatgggca tgccccggga gaggtacttg
      361 cccgtcgccc atacctcacc cggagatttc gcgtacacag aggtcagttc gttcaggtct
      421 atgccagggc ttgggctggg cacgcgcgac catcgcgtac cgtcccagtg gagggtgagg
      481 gtgtgcctgc cgcccagcgt gcccaccgcc cacacatcgt tgcccgcgct gcccgatacg
      541 gcgttcaagg cagagtctat ctcacctaca ccggggatgg gcgtttgccg ccaggtcgtg
      601 ccgtcccagc gcagcgcgag cgccctccgc tcggagttgg gtccggcgta gtagcccacg
      661 gcccacacct cgattgcgtt agtctcactg ttggagatgg ctgccacccc gttcaaaacg
      721 ttgtcgccgc tgccgaagtt ggggctgggt acgtgctccc atgcgcctgc ggtgtcgctc
      781 cagtgtacga tgaacgtcct ggcgtcccgc atctggccgg ttgcggtgtc tcgcttgctg
      841 ttgctgctgt aaccgacggc ccatgcttcg tttccgagag ccgcaaccga tctcagttcg
      901 ctctcctggg cgctgatgtc aggcaccggc accacactcc acgcgccgca tacagccccg
      961 gtagacatgc ctgccgtggc cgtcgaacct ggttgagcag tagacgtggg ggtgaggctc
     1021 tcgttcgggg tggcaatggt gccgtcggcg ctccaaatgg aggcggaggt gccggtacgc
     1081 acggctatct tgttgccctc cggcgaccat tcaaaatcgc tcacctggcc gggccagacg
     1141 gattgcaact ccttacccgc ggtcgggtcc cagagccgca gagtaccgtc gtagaaagca
     1201 ggttgctggt tgacgtacat cgtgaatatg gaaggcgatt tcgctgtttc gccgtccagc
     1261 gaggcgagca gggagccgtc gggagaccag gcgatcttca ctacctcaga tgccgaccgc
     1321 atcctccgca cctgcccgcc agtggcggga tcccacactc tgatatagtg gccgtcgaac
     1381 gaggcgagca gggcaccgtc cggcgaccag gctacgccgc cgaccgcccg gtagccctgg
     1441 tctttgtaga cgtaggtagg cgtaggggta ggtgacggaa aggtgggcat gggcggtggg
     1501 gtgatgggca ggccctgggc gttggtgggc acgacg
//
LOCUS       DASRWZ010000638         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_51181,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000638 DASRWZ010000000
VERSION     DASRWZ010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51181"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..450)
                     /locus_tag="VFH60_10125"
     CDS             complement(16..450)
                     /locus_tag="VFH60_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET6314183.1"
                     /translation="MPNHQSEYSTELAVELNRLSHDVIGAALEVHRVLGPGFLESVYE
                     EALCLELELRGIPFARQAPLPVWYKGRIVGEGRMDLLVGGCLVIELKAVEVLAQIHAV
                     QLLTYLRATGHKLGLLINFNVAVLKHGIKRVVLSHPHEQNNP"
     gene            complement(521..3379)
                     /gene="ppdK"
                     /locus_tag="VFH60_10130"
     CDS             complement(521..3379)
                     /gene="ppdK"
                     /locus_tag="VFH60_10130"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET6314184.1"
                     /translation="MATTMQNEAKGTDTGHKWVYLFNEGNAKMRDLLGGKGAGLSEMT
                     NAGLPVPPGFTITTEACRAYLAGGKKYPEGMWEQALEALHAVEGQTGKRLGDPANPLL
                     VSVRSGAKFSMPGMMDTVLNLGLNPETLQGLVQLTGNERFAHDAYRRFIQLFGRIVMG
                     VEAHHFDELLDGMKAQRGVKLDTDLTADDLRQLVEQFKEAVQREAGRPFPDDPHEQLR
                     LAIEAVFNSWNSPRAFAYRNSQDIPHDLGTAVNVVMMVFGNMGNDSGTGVAFTRNPST
                     GEAKLYGEFLVNAQGEDVVAGIRTPQKIAQLEQEMPEVYNQFLEIGQRLEKHYRDMQD
                     LEFTIERSKLYMLQTRSGKRTAAAAVKIAVDMVNEGLISREEAIQRVEPDHVVQLLLP
                     RYDDKDKERAIREGKLLATGLNAAPGAATGQAIFTADKAAELGHAGESIVLVREETNP
                     EDVHGMIASKGVLTARGGATSHAAVVARQFGKVCVAGAENGGLHVDDEAGFFTAGGQT
                     VREGEIISIDGTTGEVFLGAIALIEPNYEEEHDLVELLSWADDARKLGVWANADYPRD
                     AQKAVAFGAEGIGLCRTEHMFFEEVRLPVVRQMILSAAEATRLEDAVSVAQAELGKAD
                     EDKRDAAQEALGKAQQVAQDSRAVHEYRDALTQLGEIQQGDFYGILKAMEGKPTVIRL
                     LDPPLHEFLPSFEDLLVQTTEMRVRDETDSEEYKQKEYLLGIVSQMREQNPMLGLRGC
                     RLGILYPAINEMQVRAILQASLQLLNEGLESHPEIMIPLVGHVNELRVVRDLLEKVAR
                     DVVGQAGREVPYKFGTMIEVPRAALTAGQIAEYAEFFSFGTNDLTQTTYGYSRDDAEG
                     KFLLQYVEREVLPENPFQVLDRDGVGRLVKIAVEEGRKARPDLKVGICGEHGGDPSSI
                     EFCHQAGLNYVSCSPYRVPVARLAAAQAALTSTTEKDK"
     gene            complement(3459..>3865)
                     /locus_tag="VFH60_10135"
     CDS             complement(3459..>3865)
                     /locus_tag="VFH60_10135"
                     /inference="COORDINATES: protein motif:HMM:NF025696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6314185.1"
                     /translation="ISDDDTLESFTPTTSRALAVDDFPGPAVNRTPGGNSTPRSAFRL
                     PILKALIEKGGAASIQDVLLRVEQLIGSGFMAADYEPYPATPNQRRWQVTARLARADM
                     IQDGLLKGDSAPGRWEITSAGREAFEALSRGT"
BASE COUNT          633 a         1277 c         1153 g          802 t
ORIGIN      
        1 aaggacgcgc aaagattaag gattgttttg ttcgtgtggg tgtgagagca ctacacgttt
       61 tatgccatgt tttaggacgg cgacgttaaa gttgatgagc aggccgagtt tgtgtcctgt
      121 cgctctgagg taagtaagca actgcactgc atgaatttgg gccaaaactt caactgcctt
      181 caactctatc accaggcagc cacccacaag caggtccatc ctgccttctc ctactattcg
      241 ccccttgtac caaacgggaa ggggtgcctg gcgtgcgaaa gggatccctc gcaactctag
      301 ttccagacaa agtgcttcct catacaccga ctccaggaac cccggcccaa gcacacgatg
      361 cacctccaaa gcggccccaa tcacgtcatg cgacagcctg ttaagctcta ccgctagttc
      421 cgttgagtac tcgctctgat ggttgggcac tttaaaccct cctgaatctt tgcgcgtcct
      481 ttgcgttctt tgcgtctttg cgttttcgta atcctatcgt ttacttgtcc ttctccgtag
      541 tggaggtaag ggcggcctgc gctgctgcca gacgggcgac gggcacgcgg taggggctgc
      601 aactgacgta gttgaggccc gcctggtggc agaactcgat ggaggagggg tcgccgccgt
      661 gctcgccgca gatgcccacc ttcaggtcgg ggcgcgcctt gcggccctct tccaccgcta
      721 tcttcaccag gcggcccaca ccatcacggt cgagcacctg gaaggggttt tccggcagca
      781 cctcccgctc cacgtactga agcaggaact tgccctcggc atcatcgcgg gagtagccgt
      841 aggtcgtctg cgtcagatcg ttggtgccga acgagaagaa ctcggcgtac tcggcaatct
      901 gcccggcggt gagcgccgcg cgaggcacct cgatcatcgt gccgaacttg tagggcactt
      961 cgcgccccgc ttgcccgacc acgtccctgg cgaccttctc caacaggtcg cgcaccaccc
     1021 gcagctcgtt tacatgcccc accaacggga tcatgatctc agggtgcgac tccagtcctt
     1081 cgttgagaag ttgcagcgaa gcctgtagga tagcccgcac ctgcatctcg ttgatggctg
     1141 ggtagaggat gcccaggcgg cagccgcgca ggccgagcat ggggttctgc tcgcgcatct
     1201 ggctcactat gccgagcagg tactccttct gcttgtactc ctcgctgtcc gtctcgtcgc
     1261 gcacgcgcat ctcggtggtc tgcaccagca ggtcctcgaa ggacgggagg aactcgtgca
     1321 ggggcgggtc gagcaggcgg atgacggtgg gcttgccctc catcgccttg aggatgccgt
     1381 agaagtcgcc ctgctgtatc tcgcctagct gggttagggc gtccctgtat tcgtgcacgg
     1441 cccgcgagtc ctgggcgact tgctgtgcct tgcccaacgc ctcctgcgcc gcgtcccgct
     1501 tgtcctcgtc agccttgccg agttccgcct gagcgaccga aacggcatct tccagcctgg
     1561 tggcctctgc cgccgacagg atcatctggc gcaccacggg caggcgcacc tcttcaaaga
     1621 acatgtgctc ggtgcggcac agaccgatgc cttccgcccc gaaagctacc gccttctggg
     1681 cgtcgcgcgg gtagtcggcg ttggcccaca cgcccagctt gcgggcgtcg tcagcccagc
     1741 tgagcagttc caccaggtcg tgctcctcct cgtaattcgg ctcgatgagg gcgatggccc
     1801 ccaggaacac ctcgcccgtg gtaccgtcaa tggagatgat ttcgccctcg cgtaccgtct
     1861 ggccgccggc ggtgaagaag cccgcctcgt cgtccacgtg caaaccgccg ttctcggcac
     1921 ccgccacgca caccttgccg aactgccgcg ccactacagc cgcgtgagag gtagccccac
     1981 cgcgcgccgt gagcaccccc tttgaagcga tcatgccgtg cacgtcctcc gggttggtct
     2041 cctcgcgcac cagcactatc gactccccgg cgtgccccag ttccgctgcc ttgtcggcgg
     2101 tgaagatggc ctgccccgtg gcagctccag gggccgcgtt gaggcccgta gccagcagct
     2161 tgccttcccg gatggcgcgc tccttgtcct tatcgtcata acggggcagg agcaactgca
     2221 ccacgtggtc aggctctacc cgctgaattg cctcctcgcg ggagataagc ccctcgttca
     2281 ccatgtccac cgcgatcttc accgcggcgg cggcggtcct cttgccggag cgggtctgga
     2341 gcatgtagag cttgctgcgc tcaatcgtga actccaggtc ctgcatgtcg cgatagtgct
     2401 tttcgagacg ctggcctatc tccaggaact ggttgtagac ctccggcatc tcttgctcta
     2461 gctgcgcgat cttctgcggg gtgcggatac cggccaccac gtcctcgccc tgagcgttca
     2521 ccaggaactc tccatagagc ttcgcctcgc cggtggaggg gttgcgggtg aaggccacgc
     2581 ccgtgccgga gtcgttgccc atgttgccga acaccatcat caccacgttc acggcggtgc
     2641 ccaggtcgtg cgggatgtcc tgtgagttgc ggtaggcgaa ggcgcggggc gagttccacg
     2701 agttgaacac cgcttcgatt gccagccgca actgctcgtg cgggtcgtcg gggaaggggc
     2761 ggcccgcctc gcgctgcacg gcctccttga actgctccac aagctggcgc aggtcgtcgg
     2821 cggtgaggtc ggtatcgagc ttcaccccgc gctgcgcctt catcccatcc agcagctcat
     2881 cgaagtggtg ggcctccacg cccatgacga tgcgcccaaa aagctggatg aagcgtcggt
     2941 aggcgtcgtg agcgaagcgc tcgttgccgg tgagctggac cagcccctgc aacgtttcgg
     3001 ggttgaggcc gaggttgagc accgtgtcca tcatgcccgg catggagaac ttggcccctg
     3061 agcgcaccga caccagcagc gggttcgcgg ggtcgcccag ccgcttgccg gtctgccctt
     3121 ccaccgcgtg caacgcctcg agcgcctgct cccacatgcc ctcggggtac ttcttgcccc
     3181 ccgccaggta agcgcggcac gcctcggttg tgatggtgaa gcctgggggc actggcaggc
     3241 ccgcgttggt catctcggag aggcccgctc ccttgccgcc caaaaggtcg cgcatcttgg
     3301 cgttgccctc gttgaacaag tagacccatt tgtgcccggt gtcagtgccc ttggcctcgt
     3361 tctgcattgt tgttgccatc gctttccatt ctccttcttg ctgagtaatt aggacttgat
     3421 agccctgcca acgtttccat cgaaactata tagcggttct aagtgcccct ggacagcgcc
     3481 tcgaaggctt cccgcccggc actcgttatc tcccacctgc caggtgccga gtcgcctttc
     3541 agcagcccgt cctgtatcat gtcggccctg gccaggcgcg ccgtcacctg ccagcgcctc
     3601 tggttcgggg tagcggggta aggctcgtag tccgctgcca tgaagccgga cccgataagc
     3661 tgctccacgc gcagtagcac atcctggata gaggccgctc ctcccttctc tatgagggct
     3721 ttgaggatgg gcagcctgaa ggctgacctg ggcgtgctgt taccgccggg ggtgcgattt
     3781 acagccggac cggggaaatc atcaactgct aaggccctgc tcgtcgtagg cgtaaaagac
     3841 tccagggtat cgtcgtcaga gatag
//
LOCUS       DASRWZ010000639         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_600402, whole genome shotgun sequence.
ACCESSION   DASRWZ010000639 DASRWZ010000000
VERSION     DASRWZ010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600402"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VFH60_10140"
     CDS             complement(<1..923)
                     /locus_tag="VFH60_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314186.1"
                     /translation="MYVGAVWGGGSAAFSERDPAQRAGGIARPAEDGSPLQSPGARPE
                     GGSVDLYLDDNNYEVTLGWGIPEDNINTAAIWLNRYSLAGMRYPVTLQDVHIPFPGPE
                     DGDFAGKTIRIVVYVDPNSDGNPADAVNLYAASHVIQFVEDYQYFDLNLQINQTGDLY
                     VGWEDQWAESGPAPFSYVALLDESSPRRASYVVADRSNYAPNISNLGANDYVSLISMD
                     EFGAEGNLFIHATVAEYVPEPPRCPGERFTDVCPGDYYYTPVHNLNDRGVISGYSSSP
                     PCDTASHVPCFLPNRNLTRGHGAKRVALEAGV"
     gene            complement(1399..2313)
                     /locus_tag="VFH60_10145"
     CDS             complement(1399..2313)
                     /locus_tag="VFH60_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314187.1"
                     /translation="MNTRQFSGNSIVGGVVLILLGVVLFAGTLQPFDLGWGNLWPLFL
                     AVPGVGLLGLAFGMSKERRASVVMAGTSLLLLSLFFFAFTLERAAWSQQATLWPLYVM
                     IPGLAALVAYLGSDMERPGYLMLGAVLSTISGTLLVGTVTGSLSRLAERLPSLGITDL
                     AVLYFLQTWWPAALLVAGFIPLAVSLVAGTPSRRAFMVLAGAVPFLLGGFFLATTLGA
                     LSWGDQGRLWPIYLLIPGVAALAAYCASGFEQRRYLWAALPTGLVGAIFLGLSLTGSY
                     GLLGTLWPLYLVAAGLLLLVPRVLQSHR"
BASE COUNT          531 a          770 c          802 g          436 t
ORIGIN      
        1 acccccgcct cgagcgccac tctcttagcc ccgtgcccgc gcgtgaggtt gcggttgggg
       61 aggaagcagg gcacgtggct ggccgtatcg caaggcggcg agctgctgta gcctgagatc
      121 acgccccggt cgttcaggtt gtgcaccggc gtatagtagt aatcgccggg gcacacgtcg
      181 gtaaaccgct cccctgggca gcgcggcggc tctgggacgt attcggctac cgtggcatga
      241 atgaagaggt tgccttccgc cccgaactca tccatagata tgaggctcac gtagtcgttc
      301 gcgcccaggt tgctgatgtt gggcgcgtag ttagagcggt ccgccacgac atacgaggca
      361 cgtcgtgggc tgctctcgtc cagcagggcc acatacgaga aaggtgcggg gccgctctcc
      421 gcccactggt cctcccaccc tacgtaaagg tcccccgtct ggttgatctg taggttcagg
      481 tcgaagtact ggtagtcctc gacaaactgg atcacgtggc tggccgcgta gaggttgacg
      541 gcgtcagcgg ggttgccgtc cgaattgggg tccacgtaaa cgacgatgcg gattgtcttg
      601 ccggcgaaat cgccatcctc ggggccgggg aagggaatgt gcacgtcctg caacgtcacc
      661 ggatagcgca taccggccag cgagtacctg ttcagccaga tggccgcggt gtttatgtta
      721 tcttcgggta tgccccagcc aagcgtcacc tcgtagttat tgtcgtccag gtagagatcg
      781 acgctgccac cttcgggccg ggcaccgggg ctttgcagtg ggcttccgtc ctccgcgggc
      841 cgggcgatac caccggcacg ttgcgcagga tcgcgctcgc tgaaggctgc cgaaccgccg
      901 ccccaaacag cgccaacata caaatacacg cagctacaat ggaaatagag cgacgcattt
      961 tgctgcctcc tatcttgttg ccgacacgat tgcgacgata cacccatatg cagcccgtct
     1021 ctccaggttc tgcttaccgg ctggagcgaa gcagtcgaat gaccattggg gccggtccgg
     1081 gccacggcgc gatttacctt acgggtgagg ctctttgttg agggttactt actctgcagt
     1141 gggaggtctt gcagattgag gaagtggagg aaaatgtgac tgtgaggggc agaacatgga
     1201 aattatacat taagcaggaa accatgtctc gacaatcgct tacaagctct tacaagctgt
     1261 gggagccatt catattccca ggtacaggcg tggatgcgga gaagccgccc ctgcctcgaa
     1321 caggtggcgg cttccggcta tctcgctaac atcagcggca gggggcgttc ggcatggccg
     1381 tgtagcgccc gcctccgctc acctgtggct ctgcaggacg cggggcacca gcaagagcaa
     1441 gcccgcggca accaggtaga ggggccagag cgtgccgagc aggccgtacg agccggtgag
     1501 gctcagcccc aggaagatag cacccaccag gccggtgggt agggcagccc acaggtagcg
     1561 ccgctgctcg aagccggaag cgcagtaggc ggcgagggca gccacgccgg gaatgagcag
     1621 gtagataggc cacagcctgc cctggtcgcc ccaggagagc gcgccaagcg tggtcgccag
     1681 gaaaaagcct cccagcagga acggcaccgc tcccgcgagc accatgaagg ctctcctcga
     1741 cggggtgcct gccacgagcg acacggccag cggaatgaaa ccggccacca gcaaggcggc
     1801 gggccaccag gtctggagga agtagagtac ggcgaggtcg gtaatgccca gcgagggcag
     1861 ccgctctgcc agccgggaca aggagccggt cacggtgccc accaggagcg tgcccgatat
     1921 ggtggagagg acggccccca gcatgaggta gccgggccgc tccatatcgg agccgaggta
     1981 ggccaccagc gcggccaggc cggggatcat cacgtacagg ggccagaggg tcgcctgctg
     2041 gctccaggcc gcgcgctcga gggtgaaggc gaagaagaaa agcgagagca acagaaggct
     2101 cgtcccggcc atgaccacgc tcgccctgcg ctccttgctc atgccgaacg ccagcccgag
     2161 caagcccacg ccgggcacag ccaaaaacag gggccacagg ttgccccagc ccaggtcgaa
     2221 cggctgcagt gtgcccgcga acaatacgac tccgagaagg atgagcacca ccccgcccac
     2281 tatgctgttg ccactaaatt gcctggtatt catacctcac acccattccg gagacccacc
     2341 catattgcta ctcttatctt agctatatac ccaccgaatc ctatcgtaca aatggagtag
     2401 aatccctgat gcatatgcat gattgatatt gatgatgcta ggaagtgccg agtgccgagt
     2461 gcctagtacc aagtaccaag tactgagtgg tcagtgggca gtgctcagtg acacgacgcc
     2521 aaaacctcaa tcttctttg
//
LOCUS       DASRWZ010000640         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_874680, whole genome shotgun sequence.
ACCESSION   DASRWZ010000640 DASRWZ010000000
VERSION     DASRWZ010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874680"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1858)
                     /locus_tag="VFH60_10150"
     CDS             complement(152..1858)
                     /locus_tag="VFH60_10150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HET6314188.1"
                     /translation="MPNFTSTSARPHRFVPDGFDPADPDAVAAQYDRLDARDVSTRQA
                     LEDFILDWQELGAVLEQEYVISYVDMTADTADEARERRYIAFLENILPITEQRGFNVK
                     QKIAASPAVGELGPDYEVLLRDLKSEVTLFREENVPLIMEDLKLGQEYEKVSGNETAE
                     FQGQTYTLTQLKPFLEEPDRSVREGAWRARVEARLADAPLYDEMFDKMLELRQQIARN
                     AGFDNYLQYKFQEYKRFDYTPEDCLAFHAAIEKHIVPFTVRERERRRTLLGVETVRPW
                     DTEVDPEGHQPLRPFESADRLKEGGLRIVKQVDPQLGDFLESMVRDGLLDLENRPGKA
                     PGAYCQSFADSRVPYIHMNAVGIKVDVDTLLHEGGHAFHYFLARDLPLHSYHNPGMEF
                     SEVASMSMELLARPYLGEFYSEEELDRLLDDQLRGSLSMMPWIAMLDAFQHWVYTAEE
                     RGAEARRAKWREIEQRFRPDLDWSGLEQYRDIGWQYLHVFVVPLYYVEYGIAQIAALQ
                     VWLRSLEDPKGAIEAYKRGLSLGGSRPLPELFSAAGAEFDMGDKVVSMVTERTVAQIK
                     HK"
     gene            complement(2058..>3219)
                     /locus_tag="VFH60_10155"
     CDS             complement(2058..>3219)
                     /locus_tag="VFH60_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314189.1"
                     /translation="LRRLMGLVMLLAAALPLTAPGYAQTQSKIGSQGGGEVPPAAENT
                     QPGKSAAEPAAPGACGINFSDVHATDHYYEGVRYLYCQGAISGYTDNTFRPGNLTTRG
                     QVAKIIVLAKGWTLSNPANPSFWDVPRGSTYYTYVETARAHSVLSGYSDGSFRLGDNV
                     SRGQLAKIVSVAQGWTLVNPGTASFSDVQPGSTFYQYVETVASRQIIGGYTDNTFRPS
                     NAATRGQISKIVYYAITGLTPPPPPPPPTATPVPAGFQLTTQERQMVDLINQRRAGMG
                     LPTLRVNEQLTMATRRHSADIGPKRLCQHDGTDGSSPWSRASDAGYTGFASGEVVACN
                     YSTAQGAVDGWWNSPGHYAILTGASPNEIGCGWWINSEGYGWQTCLVGTSIN"
BASE COUNT          515 a          985 c         1092 g          627 t
ORIGIN      
        1 atcatgcagt agatgctgcc cttacccctt actaaacctt cccgatctcg gcgtgtcctc
       61 tgcgctctct gcgctctctg aggtgaaccg ttcttaatct tggcgcgctc ttggcgtcct
      121 tggcgtcctg gcggttccgt ctctttagct gttacttgtg ctttatctgc gcgaccgtgc
      181 gctccgtgac catgctcacc accttgtcgc ccatgtcgaa ctcggcaccg gcggcggaga
      241 acaattcggg gagcgggcgg gagccgccga gggagaggcc gcgcttgtag gcttcaatcg
      301 cgcctttggg gtcttccagg gagcgcagcc acacttgcag ggccgcgatc tgcgcgatgc
      361 cgtactctac atagtaaagg ggcaccacaa atacgtgcag gtactgccaa ccgatgtcgc
      421 ggtactgttc gaggccggac caatcaaggt cggggcggaa gcgctgctcg atctcgcgcc
      481 acttggcccg ccgcgcctcc gcgccgcgct cctccgccgt atagacccag tgctgaaacg
      541 catcgagcat ggcgatccag ggcatcatgg agagcgagcc tcttagctgg tcgtcgagca
      601 ggcggtcaag ctcctcctcg ctgtagaact cgcccaggta gggcctggcg agtagctcca
      661 tgctcataga ggccacttcg gagaactcca tgccggggtt gtggtagcta tgcaggggca
      721 ggtcgcgggc caggaagtag tggaaggcgt ggccgccctc gtgcagcagg gtgtccacat
      781 cgaccttgat gccgactgcg ttcatgtgga tgtagggcac gcgactgtcc gcgaagctct
      841 ggcagtacgc gccgggggcc ttgccggggc ggttctcaag gtcgagcagg ccgtcccgca
      901 ccatcgactc gaggaagtcg ccaagctgcg ggtccacctg ctttacgatg cgcagcccgc
      961 cctccttgag ccggtccgcg ctctcgaagg ggcgcaacgg ctggtggcct tcggggtcca
     1021 cctcggtatc ccaggggcgc accgtctcca cgccgagcag cgtacgcctg cgctcacgct
     1081 ctcgcaccgt gaagggcacg atatgcttct ctatcgcggc gtggaaggcc aggcagtctt
     1141 cgggggtgta gtcgaagcgc ttgtactcct ggaacttata ttggaggtag ttgtcgaaac
     1201 cggcgttacg ggctatctgc tgcctcagct ccagcatctt gtcgaacatc tcgtcgtaca
     1261 ggggggcgtc ggccagccgc gcttccaccc gcgcccgcca ggcaccctcg cgcaccgagc
     1321 ggtccggctc ctcaaggaag ggctttagct gggtgagggt gtaggtctgg ccctggaact
     1381 cggcggtctc gttgccggat actttctcgt actcctgtcc cagcttgagg tcctccataa
     1441 tcagcggcac gttctcctct cggaagagcg ttacctcgct tttgaggtcg cgcagcagca
     1501 cctcgtagtc ggggccaagc tcgcccacag cgggggaggc ggcgattttc tgcttgacgt
     1561 tgaagccgcg ctgctcggta atgggcagga tgttctccag gaaggcgatg tagcgccgct
     1621 cgcgcgcctc gtcggcggta tcggcggtca tatcgacgta ggagattacg tactcctgct
     1681 cgagtacggc gccaagctcc tgccagtcga ggatgaagtc ctcaagcgcc tggcgggtgg
     1741 acacgtcgcg ggcatcgagc ctgtcgtact gggcggcaac ggcatcgggg tcggcggggt
     1801 cgaagccgtc gggcacgaag cggtggggcc gcgcgctggt ggaagtgaag ttaggcaaag
     1861 gggttctcct ccggatgtta gtctagccag tatacagtct gaccatgcag gtagacaacg
     1921 atgcaggggc tgcacaagaa gccttgtaac aaagaaaccc gttgcacatc agcaggtaag
     1981 ccccgccttt gtcatgtaca aaggccggga gcaaatgccc tcggcctctt ctttcgcgct
     2041 cgattgtagt tgtaccccta attaatggac gtgcccacca gacaggtctg ccagccgtag
     2101 ccctcgctgt tgatccacca gccgcagccg atctcgttgg ggctggcccc tgtgaggatg
     2161 gcgtaatggc cggggctgtt ccaccagccg tctaccgcgc cctgggccgt gctgtagttg
     2221 catgcgacca cctcgcccga ggcaaagccg gtgtagcccg cgtcgctggc gcggctccag
     2281 ggcgacgaac cgtcggtgcc gtcatgctgg cagagtcgct tggggccgat atcggccgag
     2341 tgcctgcggg tcgccatcgt aagctgctcg ttcacccgca acgtgggcag gcccataccg
     2401 gcgcggcgct ggttgatcag gtccaccatc tgccgctcct gcgtcgtcag ttggaacccg
     2461 gcggggaccg gggtcgcggt cgggggaggc gggggtggag gcggggtgag gcccgtgatg
     2521 gcgtagtaga cgatcttcga gatctgcccg cgagtggccg cgttgctggg gcggaaggtg
     2581 ttgtccgtgt agccgccgat gatctggcgg gaggcgaccg tctctacgta ctggtagaag
     2641 gtcgagccgg gctgcacgtc ggagaagctg gccgtgccgg ggtttaccag ggtccaaccc
     2701 tgcgccaccg acacaatctt ggcaagctgg cccctcgata cgttgtcgcc caggcggaag
     2761 ctgccgtccg agtagccgct gaggacggag tgcgccctgg cggtctcgac gtatgtgtag
     2821 taggtgctgc cgcgcggcac gtcccagaag ctaggattgg cggggttgct cagcgtccag
     2881 cccttagcca gcacgatgat cttggctacc tggccgcggg tggtgaggtt gccggggcgg
     2941 aaggtgttgt ccgtgtagcc ggagatggca ccctggcagt agagatagcg cacgccctcg
     3001 tagtagtggt cggtggcgtg cacgtcgctg aagttgatgc cgcacgcgcc aggggcggca
     3061 ggctcggcgg ccgatttgcc cggctgtgtg ttctcggccg cggggggcac ttcgccccca
     3121 ccctgcgacc cgatcttgga ctgagtctgt gcgtagccgg gggccgttag aggtaaagcc
     3181 gccgcgagca gcatcacgag gcccatgagg cggcgcagc
//
LOCUS       DASRWZ010000641         3918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_120351, whole genome shotgun sequence.
ACCESSION   DASRWZ010000641 DASRWZ010000000
VERSION     DASRWZ010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120351"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1137)
                     /locus_tag="VFH60_10160"
     CDS             complement(91..1137)
                     /locus_tag="VFH60_10160"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HET6314190.1"
                     /translation="MNDSDWLERIRGLVGGHQRTVAHSGISREVRNRPERGRVASFLL
                     MGFLALAVVATGFYGLAGRNAGSALTTVIAPADGDVNYMMTVPSEEAAVEGFRAPEYA
                     AKAGQEGQDSQRWDRMIIRTATLQLRVKDVAASMDEIRGVVGGHAGYVTGSESRQEGE
                     STVGTMTVQVPAAQFDAAISKLRTLGLKVLHENVTTSDVTEEYTDLNSQLRNLQATES
                     RILALVGRAEQINDILALDRELRGIQGEIERIQGRLNFLGKRAEMSTITISLYPEAVV
                     VEPAATPAEAWDPVRIANQAWDASLQLLANVGTAVITVVVYLWWLLPLGMLAAVFLRR
                     ARRTMPTAPAATTE"
     gene            complement(1434..1748)
                     /locus_tag="VFH60_10165"
     CDS             complement(1434..1748)
                     /locus_tag="VFH60_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET6314191.1"
                     /translation="MTKIDISSGVVHEVPADLRDALTSDPEALAAWENLTPLARNEWI
                     CWTISVKKPETRREHVEKVCTELKEGIRRPCCWYGCIHRTDKSVSPSVQAVVLDRRSK
                     SK"
     gene            complement(1843..2343)
                     /locus_tag="VFH60_10170"
     CDS             complement(1843..2343)
                     /locus_tag="VFH60_10170"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6314192.1"
                     /translation="MDFIGTARRQMLAGQLQDGRERLVNSYAGLTDEEMLEPGVAGDW
                     SVRDVLAHVAAWDRAQTEAYRQMLRGERPALLDLDEEGNEAFNLENHERMKDLGMQEV
                     ITELFAAREEMVELLREVDNAALYSPAPGDEHAELSIAACISVSASHDEEHAEMIEDW
                     RDSRSQ"
     gene            complement(2348..3541)
                     /locus_tag="VFH60_10175"
     CDS             complement(2348..3541)
                     /locus_tag="VFH60_10175"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET6314193.1"
                     /translation="MASNNNNPVILAGVRTPFGKFGGSLAPLGAVELGAHVMREAMGR
                     ADIDTNDVTHNIMGCVLQGGLGMIPSRQAAFAAGLGREVTSDTVNRVCGSGIRSIELA
                     NDLIRMGHHSLVVAGGMESMSNAPFYSFGARWGLRMGHGELRDAILHDGLTDPLMKVH
                     MGTHGSQVAAEEECSREQQDAWALRSHQRALAAIDNGIFAQEIAAVTIPGKKGDTVVD
                     TDEMPRRDTSMEALAKLKPVFDEKGTVTAGNAPGINDGAAAMVIASSEYAKAHGLKPI
                     ARILGYGEAAWDTPYLAYVPAMAGQNALKKLGMTVDDLDLVEINEAFASVALISIAKL
                     GISEDKVNVNGGAVALGHPIGASGARILLTLIYELQRRGGGIGMAAICSGTAQGDAVI
                     VQVGE"
     gene            complement(3626..>3918)
                     /locus_tag="VFH60_10180"
     CDS             complement(3626..>3918)
                     /locus_tag="VFH60_10180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6314194.1"
                     /translation="DAIEIHGAYGYSSEYPVERYLRNARGAVIYEGTREVHTVMQGEY
                     ALGYRQDRPYRCPPPPAVGYERKDAYDPVSALHYPFPFPMKTEEGATPTPGN"
BASE COUNT          666 a         1277 c         1156 g          819 t
ORIGIN      
        1 cctaatttca gcatcggttc gagcagtcgt gctacgagag gaacggcggg agggaccaat
       61 aatacctccc gccgttcctt tagaaaagcc ctattcggtg gttgccgcag gtgctgtggg
      121 catagtccgc cgggcgcggc ggaggaacac ggcggcgagc atgcccaggg gcagcagcca
      181 ccacaggtac accaccaccg taatgaccgc cgtgcccacg tttgcgagca actgaaggga
      241 ggcgtcccaa gcctggttag ctatcctcac cgggtcccac gcctcggccg gggtagcggc
      301 tggttcgacc acaaccgcct cagggtagag gctgatcgtt atggtagaca tctcggcgcg
      361 cttgcctagg aagttgagcc ggccctgtat gcgctcgatc tcgccctgga tgccgcgcaa
      421 ctcgcggtcc agcgccagga tgtcgttgat ctgctcggca cgtcccacca gggccaggat
      481 gcgcgactcg gtggcctgga ggttgcgcaa ctgcgagttg aggtcggtgt attcctcggt
      541 cacgtcgctg gttgtgacgt tctcgtggag caccttcagc cccagggtgc gtagcttgga
      601 gatagcggcg tcaaattggg ccgcaggtac ctgcacggtc atcgtgccga ccgtggactc
      661 gccttcctgg cggctctccg acccggtgac atatcccgcg tgcccgccca ccacgccgcg
      721 tatctcgtcc atgctggcag ctacgtcttt tactctcagt tgcagggtgg ccgtcctgat
      781 aatcatgcgg tcccagcgct gactgtcttg cccctcttgc cccgccttgg cagcgtattc
      841 cggcgctcta aagccctcta cagcggcctc ctcgctcggc accgtcatca tgtaattaac
      901 atcaccgtct gcgggtgcaa tgacggttgt cagcgcagac cccgcgttcc ggccggctaa
      961 accatagaag ccggtcgcca ctaccgcgag cgctaggaag cccatgagca ggaaggacgc
     1021 tacccgtcct cgctccggtc tgttccgcac ctcacgcgat atgccgctgt gggctacggt
     1081 ccgctgatgt ccccccacca gccctcgaat tctctctagc cagtctgaat cgttcatcgt
     1141 acgcctcccg tgcttggcta agctttgtag tcacctggca cattgttgag tgctacttag
     1201 tgacgccggg aggcgcaact ttgttccacc gcgcatatct aaacgccaag acgccaagac
     1261 gccaagggcc acgccaagtt gagtatggtg ttccaagtaa aaggaccagg gtacattagc
     1321 acgtaccctg gtcccatttg aactcaccga acgctcctaa actttgcgcg ttctttgcgt
     1381 cctttgcgtc ttggcgtcta gagcctgtta tgcacttttc ggcggcaaac aagctatttg
     1441 gacttagatc gtctatcaag taccactgcc tgtaccgaag gactaactga cttatctgtc
     1501 cggtgaatac agccatacca acagcaaggt cggcgaattc cctctttaag ttctgtacac
     1561 accttttcaa catgctccct tctggtttcc ggcttcttaa cggagatggt ccagcatatc
     1621 cattcattgc gtgcaagggg tgtaagattc tcccatgctg cgagcgcttc cgggtcagat
     1681 gtcaaagcat cccgtaaatc cgctggaact tcgtgcacta caccgctaga aatatctatt
     1741 ttggtcatag ggctattata acagaacccc tttggacgta ttgggtgaca ggcgaaacgg
     1801 tgaaagtggt ggaatagttc ataacaggct ctagatagaa agctattggc tgcgagagtc
     1861 ccgccagtcc tctatcatct cggcgtgctc ctcgtcgtgg ctggccgaga ctgagatgca
     1921 ggcggctatg gaaagctcgg cgtgctcgtc gccgggggcg ggggagtaga gggcggcgtt
     1981 gtccacctcg cgcaatagct cgaccatctc ctcgcgggcg gcgaatagct cggttatgac
     2041 ctcctgcatg ccgaggtcct tcatgcgctc gtggttttcg aggttgaaag cctcgttacc
     2101 ctcttcgtcc aggtcgagca gggcggggcg ctcgccacgg agcatctgcc tgtaagcctc
     2161 cgtctgcgcg cggtcccagg cggccacgtg ggccagcacg tcgcgcaccg accagtcgcc
     2221 cgccaccccc ggctccagca tctcctcgtc ggtcaggccc gcgtaggagt tgaccagccg
     2281 ctcgcgccca tcctgtagct gcccggccag catctgccgc cgggccgtgc ctatgaagtc
     2341 caaggcttta ctcgcctacc tgcacgataa cggcgtcgcc ctgcgcggtg ccggagcaga
     2401 tagcagccat tcctatgccg ccgccgcgcc gctgtagctc gtagatgagc gtcagcagga
     2461 tacgggcacc ggacgcgcct atcgggtggc ccagcgcaac cgcgccgccg ttcacgttca
     2521 ccttgtcctc ggagatgccc agcttggcta tgctgatgag ggccacgctt gcgaacgcct
     2581 cgttgatctc caccaggtcg aggtcgtcca ccgtcatgcc cagctttttg agggcattct
     2641 gcccggccat cgcgggcacg taggccaggt aaggggtgtc ccaggcggcc tcgccgtagc
     2701 cgagtatgcg ggcaatgggc ttcagcccgt gggccttggc gtactccgat gaagcgatca
     2761 ccatagccgc cgcgccgtcg ttgatgccgg gggcgttgcc cgccgtgacc gtgcccttct
     2821 catcgaacac cggcttgagc ttcgcgaggg cctccatcga ggtgtcgcgc cggggcatct
     2881 cgtcggtgtc caccaccgtg tcgcccttct tgccggggat ggtcacagcg gctatctcct
     2941 gggcgaagat gccattatct atggcagcca gggcgcgttg gtgggagcgc aaggcccagg
     3001 cgtcctgctg ctcgcggctg cactcttcct cggcggccac ctgcgagccg tgagtgccca
     3061 tgtgcacctt catcagcggg tcggtcagcc cgtcgtgcag gatagcgtcg cgcagctcgc
     3121 cgtgccccat gcggaggccc cagcgagcgc cgaaggagta gaacggggcg ttcgacatgc
     3181 tctccatgcc acccgccaca accaggctgt gatgccccat tcgtatcagg tcgttggcaa
     3241 gctcaatcga gcgtatgccg ctgccgcaca ctcggttcac cgtatccgat gtgacttcgc
     3301 gccccagccc cgccgcgaac gccgcctgcc gcgaggggat catgcccagg cccccctgca
     3361 acacgcagcc catgatgttg tgcgtaacat cgttcgtgtc gatgtcggcg cggcccatcg
     3421 cctctcgcat cacgtgcgcg cccaattcca ccgcgccgag gggtgccagc gacccgccga
     3481 acttgccgaa cggggtgcgc acccccgcga gtatcaccgg gttgttattg ttgcttgcca
     3541 tgtatgttgc ctcctgtacg tcgccgtaac gtccgtgtta caacactact ctgtttgctc
     3601 tactatacaa cttcccggtt acattctagt tgccgggcgt gggcgtggcg ccttcctcgg
     3661 tcttcatagg gaaggggaag gggtagtgta acgcgctcac cgggtcgtat gcgtccttgc
     3721 gctcatagcc caccgcgggc gggggcgggc agcggtacgg cctgtcctgc ctgtagccca
     3781 gcgcgtactc gccctgcatc accgtgtgga cctcgcgggt gccctcgtag atgaccgccc
     3841 cacgcgcgtt gcgcaggtac cgctccactg ggtactcgga cgagtagcca taggccccgt
     3901 ggatctcaat cgcgtcgt
//
LOCUS       DASRWZ010000642         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1353225, whole genome shotgun sequence.
ACCESSION   DASRWZ010000642 DASRWZ010000000
VERSION     DASRWZ010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353225"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1583)
                     /locus_tag="VFH60_10185"
     CDS             complement(201..1583)
                     /locus_tag="VFH60_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6314195.1"
                     /translation="MQDLTSLTATQLALSIRERQTSSVEAVEAYLARIAVRNPALNAI
                     VTLDAEGALRRAAECDEAQARGEMWGPLHGVPMTLKDGHSTAGMRTTCGYPPLAEYVP
                     TEDGTVAARLKEAGAIILGKTNVSPLLGDIQSDNDIFGRTNNPYDLERTPGGSSGGAA
                     AALAAFMTPLEIGSDMAGSIRIPAHFCGLFGLKTTEHRVSSYGHIPDLPGALRSHGLM
                     GTIGPLARSLDDLELAYRVIAGPDGHDHAVPPIPLREVPHLSPGELRVAWAPTFPGVP
                     VAAAIREAVHRVAAELERAGAQVEETLPDVDFRQLARARAVLSSAMGLPAEPEPGEEV
                     PDLRRYYNALHLRDAIISAWEAFFGEFDALLCPVCMVTAFPHCPTNSALQVDGETVNY
                     WRALGHTAPFNFTGHPVVVVPIGKDAGGLPIGMQVVGKRWDEERLLGIARLLEAIPGV
                     GMLGQPALRD"
     gene            complement(1906..>2099)
                     /locus_tag="VFH60_10190"
     CDS             complement(1906..>2099)
                     /locus_tag="VFH60_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314196.1"
                     /translation="NAMGVKGAGEAGAIPVGPLYAQALDNAFGDIALEIREIPLSPSK
                     LWELVEAARKGRSAGGARS"
BASE COUNT          347 a          698 c          655 g          399 t
ORIGIN      
        1 aagctgctaa attgaggctg atgaacgtta cctcttatgg gctgacgttc agcgtagagg
       61 cagaagtagc atcttagatt cttcgctacg ctcagaatga caagatccac ctttgttcgc
      121 ttgcttccta cactttgcaa atatgccctg gcacactgca tgccggttat tgtggtttat
      181 tccctggcag gccacatcaa ttagtctcta agtgctggct ggccgagcat ccctacaccc
      241 ggaattgcct caagcaacct tgctatcccc agcagacgct cctcgtccca ccgcttcccc
      301 accacctgca tacctatagg cagaccgcct gcatctttcc cgattggcac caccactacg
      361 gggtggccgg tgaagttgaa gggggcggtg tggcccaggg cgcgccaata gttcaccgtc
      421 tcgccatcta cctgcagggc actattggtc ggacagtgcg ggaaggccgt gaccatgcac
      481 acggggcaga ggagcgcatc gaactcaccg aaaaaggcct cccacgcgct gatgatggcg
      541 tcgcggaggt ggagagcgtt gtagtaccgc cgcaggtccg gcacctcctc gcccggctcc
      601 ggctccgcag gtaggcccat cgcggaggag agcacggccc gcgccctggc gagttgcctg
      661 aagtccacgt cgggcagcgt ctcttccacc tgcgccccag cccgctccaa ttcggcggca
      721 accctgtgga ccgcctcgcg tatggcagcg gccaccggca cgcccggaaa cgtaggtgcc
      781 caggcgacgc gcaactcccc cggcgatagg tgcggcacct cgcggagagg gatgggcggc
      841 acggcgtggt cgtgtccgtc cggcccggcg attacgcggt aggcaagctc caggtcgtcc
      901 aggctgcggg cgagcgggcc tatcgtcccc atgaggccgt ggctgcgtag cgcgcccggc
      961 aggtcgggta tgtggccgta actcgacacg cggtgctcgg tcgttttcag cccgaaaagg
     1021 ccgcagaagt gggccgggat gcgtatcgag cctgccatgt cactgccgat ttccaggggg
     1081 gtcatgaacg cagccagggc cgccgctgcc ccaccgctcg agcctcccgg cgtgcgctca
     1141 aggtcgtacg ggttgtttgt gcggccaaag atgtcgttgt ccgactggat gtctcccagc
     1201 aggggcgaaa cgttggtctt gccgaggatg atagccccgg cctccttcag ccgcgccgcc
     1261 acggtgccgt cctcggtggg cacgtactcg gccaggggcg ggtagccgca ggtggtgcgc
     1321 atccccgccg tggagtgccc gtccttcagc gtcatgggca ccccgtgtag cggcccccac
     1381 atctccccgc gggcctgcgc ctcgtcgcac tccgccgcac gcctgagcgc gccctccgcg
     1441 tccagcgtga ctatggcgtt cagggcgggg ttgcggacgg ctatccgcgc caggtacgcc
     1501 tccaccgcct ccacggacga tgtctggcgc tcgcgtatgg agagggcgag ttgtgttgct
     1561 gtgagggagg tcaggtcttg catggtctaa gcgtcaggta tgcgagtgcc ggaaggtggg
     1621 cttgcgggtg tattggcagg acttaccacg tgctggtagc tagctcgcta cctcgtagta
     1681 cgggaccaaa gcggccttgt catcctgagc gcagcgaagg atcagccgcc cgtgttcgac
     1741 tcttcctatc ttggcaccag tgttcctctt accacgctca tccccaacgt tgtagactct
     1801 cgcttaccca cctgatcctt cgctgcgctc aggatgtcaa agggaggctt ggtcctacaa
     1861 caagaaagat aggggctaac cacgagggta cgaggcctgg tgctcctacg agcgggcacc
     1921 gcccgccgag cggcctttgc gcgctgcctc caccagctcc cacaacttgc tggggctgag
     1981 cggtatctcg cgtatctcca gcgcgatatc cccaaaggcg ttgtctagcg cctgcgcgta
     2041 cagcggcccc accgggatgg cccccgcttc gcccgcgccc ttgaccccca tcgcgttca
//
LOCUS       DASRWZ010000643         5354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_325689, whole genome shotgun sequence.
ACCESSION   DASRWZ010000643 DASRWZ010000000
VERSION     DASRWZ010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325689"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..538
                     /locus_tag="VFH60_10195"
     CDS             167..538
                     /locus_tag="VFH60_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET6314197.1"
                     /translation="MSKRRRGYPSERAVKRGKRVVHGHKELTEKLGKDDPCPCGSGRR
                     FQAVLTQLGQLRRLRARLLLLENSGRGVHHLHAPFILPYLANLVLTSGSLPRHLRCPQ
                     NHHHRSLGRLLAMSSDLPPMY"
     gene            complement(710..1276)
                     /locus_tag="VFH60_10200"
     CDS             complement(710..1276)
                     /locus_tag="VFH60_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET6314198.1"
                     /translation="MIECKIESIRVSLVTQQRVVILKEVDSERYLPIWIGPYEADAIA
                     LELQEVPVQRPFTHDLLRNVISELGASVTHVLVNDLHDDTFFARIVLDVQGRHAEVDS
                     RPSDAIALGVRCKCPIYVEERVMDKAGITIDTDGASKDAEEVPANESLSQGERVDEDR
                     LSVFRDFINSLDIDDLGNKGGADDPRRS"
     gene            1877..4441
                     /locus_tag="VFH60_10205"
     CDS             1877..4441
                     /locus_tag="VFH60_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET6314199.1"
                     /translation="MNDKSLHTLEFHKVRERLASYASFSAGKTVALELVPLTDITQVR
                     RNQRITSEAVRLLSLRPDVTLGGARVFREMVPRSELGSVLDPGEVLMVLDTLRACRIL
                     HGVITRTAEQRGELPTLAFIADGLTLLPKLEQEIERCISEDGQVLDSASPALGKIRVG
                     IRAAHNRLMQRLQQLISSEAAGRALQEPIITLRNGRYVVPVKRDFQGMLKGIVHDQSN
                     TGSTLFVEPLAVVDLGNEWRQLQLEEQQEIERILRELGGLIGEKGDAIRTNVEILADI
                     DLALAKARYSIAIDAREPELVEPRIKGPDPTRPLDNRERRQVHTQPRPPQDDQSNQIQ
                     NPKSKIQNPQGLYFKNARHPLLTGKVVPITIELGGRFRMLVITGPNTGGKTVALKTVG
                     LLTLMSQAGMHIPAEDGSRAVVFKDVFADIGDEQSIEQSLSTFSSHMSNIVGILRRAT
                     RDTLVLLDELGAGTDPQEGSALARAIISTILDIGALAVCTTHYSELKAYAFSTPNVEN
                     ASVEFDIETLSPTYRLMIGVPGKSNAFSIASRLGLNPMVIDKARRYIDPESQRVEDLI
                     EDIRTQREAAKSELGTAEQARREMDQRRQELDRRLAEADELKARAAEEGRAQLEGELE
                     TLRSELRQLRARVEAGLHATSDETLTRQWVKDAQVRAEEMERELRSKANAQKKARNRP
                     QPSKGPRAGEAGKPHVLSPGDMVFVQSLSSEGEVLTQPDGSGQVDVQVGAFKMRVPVA
                     NLSLRSTASAASAAREAASAGMARSYNTPAMPTETPRLEYDYRGWRAEEALEDLDQRL
                     NDATLVGMPFIRIIHGKGTGALRQAMREYLRKHPVVKEIEPAPVEQGGEGVTIVKL"
     gene            4775..>5354
                     /locus_tag="VFH60_10210"
     CDS             4775..>5354
                     /locus_tag="VFH60_10210"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET6314200.1"
                     /translation="MNPHAQWRLELARRIAPAYSGNPSVRAVAVHGSAARGYADKYSD
                     IEIVAFWDLAPTDEERIAAVEGAGGNLWNLYPFEDENNEWSEEFDIRGVKIDMTHRTV
                     EGTETWFADVIERYDTDLTKHDMISVVRRCYPLYGAELIEQWRARTDVYPDELARLMV
                     SKYLDWIPAATRVMAAARGELIPLYENIAVSER"
BASE COUNT         1095 a         1621 c         1685 g          953 t
ORIGIN      
        1 ctgctcacaa actctccgta ttgccgcgaa gcgtcgaggg cgcgccacac aaagctactg
       61 taccgtagcc ccccaggcgt gtcaaggtcg ccgtaccgcc tcccaggatg gtcccacaga
      121 ccgattgacg cgaaggagac cgccgagggg cacggctttt gagccgatga gtaagaggcg
      181 acgcggctac ccatccgagc gggcggtgaa gcgtggtaag agggtagtgc acgggcacaa
      241 ggagcttact gagaagctgg gcaaggacga cccctgcccc tgcggctcgg ggaggcgctt
      301 tcaagcggtg ctgacgcaac tcgggcaact acgacggctc cgagcgcgat tactactttt
      361 agagaatagc ggaaggggcg tgcatcacct gcacgcccct ttcattttgc cctacttagc
      421 caacctggtg cttacgtctg gatctcttcc tcgtcacctt cgttgtcctc agaaccacca
      481 ccatcggtct ttgggacggt tgcttgccat gtcatcggac ttacctccta tgtattagaa
      541 cccgacctgc cggacgccgc agtgttgaat ggggcaagta tatcacagga tgtagtatac
      601 gtacagactc cacaagccaa gagacgaagg ggccgcgcac cagtggcaca acccctcccc
      661 aaatcacacc ctaattcgaa atccgaaatc cgacattcgc aattcctact tagcttctcc
      721 gcgggtcgtc cgcgccgcct ttgttgccga ggtcgtctat atcgagggag ttgatgaagt
      781 cgcggaagac ggagaggcgg tcttcgtcca cgcgctcgcc ctgggagagg gactcgttgg
      841 caggcacttc ttccgcgtct ttggaggccc catcggtgtc gatggtgata cccgccttgt
      901 ccatcacgcg ctcttccacg tatatggggc acttgcagcg cacgccgagg gctatggcgt
      961 cggatgggcg ggagtcgacc tccgcgtggc ggccctgcac gtcgaggacg atgcgggcga
     1021 agaaagtatc gtcgtgcaag tcgttcacga gcacgtgggt cacggacgcg ccaagctcgg
     1081 agatgacgtt gcggagcagg tcgtgcgtga acggccgctg tacgggcact tcctgcaatt
     1141 cgagcgcgat ggcgtcggcc tcgtacggtc cgatccagat cggcaggtac ctttcggagt
     1201 ccacctcctt cagaatgacc acgcgctgct gagtaaccag gctcaccctg atgctctcta
     1261 tcttacactc aatcatcgac ttaccgcctc catctagcac agggaagaag aaaatagaat
     1321 tacgcttgcc ttcactgcta ttgtactgtc tttttcctgc ctttgtaaag acataacgag
     1381 ggttgggagc gccgggtagg aagtctatgt cctgcaattc gcaggccacg ctttgccgtt
     1441 gtcacctagt tgtcacctgt attaacgcag gcgacttgaa atgagtttca gccgcgcggt
     1501 gcgccaatct atacctctga ttgggcgcaa agcctcacag ggtggcgcgt cagcacctga
     1561 aggtgatatt gtcattctga gcgtagcgag gaatcaggtg ggtagggaag aaggcgcatt
     1621 ggaggactgg cgtggccttg cgccctctat tccttccaat tcaacgctga acgactgcca
     1681 cctgattcct cgctacgctc ggaatgacaa gatggacgag ggtactacta attgcggcag
     1741 cttgcggcag ctatgaccct gacatagccc caatctacac ctctcgtcgc atgcgggagg
     1801 gctgccccga atggtataat tgactgtatg cagtactgta gctttatcat gcccgaaaca
     1861 tgggaccgga aagacattga acgataaatc ccttcacacg ctagagtttc acaaggtacg
     1921 cgagcggctt gcctcctacg cttcgttcag cgcgggcaag acggtggccc tcgaactcgt
     1981 gccgctcacc gacatcaccc aggtgcggcg caaccagcgc atcacgagcg aggccgtgcg
     2041 cctgctgtcg ctacggcccg acgtgacgct cggcggggcg cgggtcttcc gcgagatggt
     2101 gccgcggtcg gagcttggct cggtgctcga ccccggcgag gtgctgatgg tgctcgacac
     2161 gctgcgggcc tgccgcatcc tgcacggcgt catcacgcgc acggcggagc agcgcggtga
     2221 gcttccgacg ctggctttca tcgcggacgg cctgacgctg ctgcccaagc tggagcagga
     2281 gatagagcgt tgcatcagcg aggacgggca ggtgctcgat tctgcctccc ccgcgctggg
     2341 caagatacgg gtgggcatac gcgcggcaca caaccgcctg atgcagcggc ttcagcagct
     2401 tatatcgtcc gaggcggcgg ggcgggcgtt gcaggagccg attatcacgt tgcgcaacgg
     2461 ccgctacgtc gtgcccgtga agcgggactt ccagggcatg ctcaagggca tcgtgcacga
     2521 ccagtcgaac accgggtcca ccctcttcgt ggagccgctg gccgtggtgg acctgggcaa
     2581 cgagtggcgg cagctacagc ttgaggagca gcaggagata gagcgcatcc tccgcgaact
     2641 gggcggcctg attggcgaaa agggcgacgc gatacgcacc aacgtcgaaa tcctggccga
     2701 catcgacctg gccctcgcta aagcccgcta cagcatagcc atagacgcgc gggaacccga
     2761 actcgtagaa ccccgcatca aaggccccga ccccacccgc cccctagaca accgcgagcg
     2821 ccgccaggtc cacacgcaac ccaggccccc acaagacgac cagtccaatc aaatccaaaa
     2881 cccaaaatcc aaaatccaaa atccccaggg tttgtacttc aagaacgctc gccaccctct
     2941 gctcaccggc aaggtggtgc cgattacgat tgagcttggc ggcaggttcc gcatgttggt
     3001 gattaccggc cccaacacgg ggggcaagac ggtggccctc aagacggtgg ggttgctcac
     3061 cctcatgtcg caggcgggca tgcacatccc ggcggaggac ggctcgcggg cggtggtctt
     3121 caaggacgtg ttcgcggaca taggcgacga gcagagcatc gagcagagcc tgagcacctt
     3181 ctcttctcac atgtccaaca tcgtgggcat cctgcgccgg gccacccgcg acacgctggt
     3241 gctgctggac gaactgggcg cgggcaccga cccgcaagag ggttcggccc tggcgcgcgc
     3301 cattatcagc acgatactcg atataggggc gctggcggtg tgcaccaccc actactcgga
     3361 gttgaaggcg tacgcgttct ccacgcccaa cgtcgaaaac gcctccgtgg agttcgacat
     3421 agagacgcta agccccacct accggctgat gataggcgtg cccggcaaga gcaacgcttt
     3481 ctccatagcg agccgcctgg ggctgaaccc gatggtgata gacaaggcgc gccgctacat
     3541 cgacccggaa tcgcagcgcg tggaggactt gatagaggac atcaggacgc agcgggaggc
     3601 ggcgaagtct gagctaggca cggcggagca ggcccgccgc gagatggacc agcgccgcca
     3661 ggagcttgac cgccgcctgg cggaagccga cgagctaaag gcgcgcgccg ccgaggaggg
     3721 ccgcgcccag cttgagggcg agcttgagac gctgcgctcc gagcttcggc agcttcgggc
     3781 gcgagtggag gcggggctgc acgctacttc cgacgagacg ctgacccgcc agtgggtgaa
     3841 ggacgcccag gtacgcgccg aggagatgga gcgcgagctc aggagcaagg ccaacgccca
     3901 gaagaaggcg cggaaccgtc cgcagccgtc gaaaggcccc agggcgggag aggcgggcaa
     3961 gcctcacgtc ctcagccccg gcgatatggt gttcgtgcag agcctctcct cagagggaga
     4021 ggtgctgacc cagccggacg gcagcgggca ggtggacgtg caggttgggg cgttcaagat
     4081 gcgggtgccg gtcgccaacc tgtcgctcag gagcacggcg tcggctgctt ccgccgccag
     4141 ggaagccgca tcggcaggta tggcgaggag ctacaacacg cccgccatgc ccaccgaaac
     4201 gccccggctg gagtacgact atcggggctg gcgcgccgag gaagcgctgg aggacctgga
     4261 ccagcgcctg aacgacgcca cgctggtcgg catgcccttc atccgcatca tccacggcaa
     4321 gggcaccggg gcgctgcgcc aggccatgcg cgagtacctg cgcaagcacc cggtcgtcaa
     4381 ggagatagag cctgcccccg tcgaacaagg cggcgagggc gtgacgatag tcaagttatg
     4441 aggcgtgacg agtgacgagt gatgagttga ggcttactga ggatagtacg tgagtgtgcg
     4501 gtagtgggtg ttacgggatc aagcccccct ttgtcattct gagcgcagcg aagaatctcg
     4561 tcttctaccg aggttttccc cttttcaatg gcagcattgt ggcatgaaga aaggggtcat
     4621 ttgggtgctg gaggacgaga ttcttcgcta cgctcagaat gacaaaggtc aacagggtcc
     4681 ctccattaca tcagtgtggc attcacgcag cagataggta agccgcctca ctcatcactc
     4741 atccctcatc actcgtcact tctgagggag atgtatgaac ccacatgcgc agtggcggct
     4801 ggagcttgcg cggcgtatag caccggctta ttccggcaac ccctcggtgc gtgctgtggc
     4861 cgtgcatggg tcggcggcgc gcggctacgc ggacaagtac tcagacatag agatagtggc
     4921 tttttgggac cttgcgccta ccgacgagga acgcatcgcg gctgtcgagg gggccggggg
     4981 caacctgtgg aacctctacc cgttcgagga cgagaacaac gagtggtcgg aggagttcga
     5041 tatacgcggc gtcaagatcg atatgaccca ccgcacggtg gagggtaccg agacctggtt
     5101 tgccgatgtt atagaacgct acgataccga cttaaccaag catgatatga tttcagtggt
     5161 gcggcggtgt tacccgcttt acggcgcgga gctaatcgag caatggcgcg cccgcaccga
     5221 cgtttacccc gacgagcttg cccggctcat ggtgagcaag tacctcgact ggattccggc
     5281 ggccacgcgc gtgatggcag cggcacgcgg cgagctgata ccactctacg aaaacatcgc
     5341 cgtctccgag aggc
//
LOCUS       DASRWZ010000644         1679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1217222, whole genome shotgun sequence.
ACCESSION   DASRWZ010000644 DASRWZ010000000
VERSION     DASRWZ010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217222"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="VFH60_10215"
     CDS             <1..1587
                     /locus_tag="VFH60_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6314201.1"
                     /translation="QAEDAIELKQALNRSVQMRFEVTHVASLSDAARWLATTEFDVVL
                     ADLYLPDSSGPRAIVEFHERAPHVPVVALATHEAESAAMEAMQAGAEDYYVKGNVDTV
                     LLVRIVRHAIERKSYTDKLQFLGEVSARLAALLDYEGTLFSIVRLAVPRLADCCLVDV
                     LDEAGKLKRVVACPPGPAVSSHEHELDIHEVEQGAGPHPTMEAVYAGKPELFPTISNS
                     ELAQLAGSAEYLKPLFDVEPKSLIRVPLMLRGSALGMLSLFVVGPHRSYEQDDLALAV
                     EFAQRCALALDNALLYRKVGQALHAREDLLASVSHGLRTPLTSIKAGLGLVEMSMSER
                     MGTDERQLITNIRRNVERLGILVNDLLTFSEIETRTFQLACKPLDLRAVVEDAVAMIE
                     PLVQKKRQVVEVDMPEAVPYNGDRTRLEQVVVNLLHNSHKYAPEGARITVSGQVTPGE
                     VLLAVRDDGPGIPIEEHEAIFERFDHGHPVRSGEGWGLGLAIARSIVEMHGGRIWVEG
                     QQGEGATFHIALPLPGEPAI"
BASE COUNT          329 a          469 c          568 g          313 t
ORIGIN      
        1 caggctgagg atgctattga gttgaagcag gcgctcaatc gctccgtcca gatgcgcttc
       61 gaggtgacgc acgtcgcatc cctgagcgat gcggcccggt ggttggcgac gacagagttc
      121 gacgtggtgc tggccgacct ctatctgccc gatagctccg ggccgcgcgc catagtcgaa
      181 ttccacgaac gagcgccgca cgtgcctgtg gtggcgctcg ccacgcacga agctgagagc
      241 gcggcgatgg aggctatgca ggcgggcgct gaggactact acgtgaaggg caacgtagat
      301 accgtcttgc tcgtgcgtat cgtccggcat gctatcgagc gtaaaagcta caccgacaag
      361 ttgcagttct taggggaagt gagtgccagg cttgccgctt tgctcgacta cgagggcacg
      421 ctcttctcta tagttcggct ggcggtgccc aggctcgccg actgctgcct ggtggacgtg
      481 ctggacgagg cgggcaagct gaagcgggtg gtggcgtgcc ctcccggccc ggcggtgtcg
      541 tcccacgagc atgagcttga tatacacgag gtcgagcagg gtgcgggacc tcacccgact
      601 atggaggctg tgtacgctgg gaagcctgag ttattcccca ccatatcaaa tagcgagtta
      661 gcgcaattgg cgggtagtgc cgagtacctc aaacccttgt tcgatgtaga gcctaagtcg
      721 ttgataagag tgcccttgat gttgcggggc agcgcgctcg gaatgctcag cctgttcgtg
      781 gtggggccgc acaggagcta cgagcaggac gacctggcgc tggcggtgga gtttgcccag
      841 cgttgcgcgc tcgcgcttga caacgcgctg ctctaccgga aggtggggca ggcgttacac
      901 gcccgtgaag acctcctcgc atcggtgtcc cacggcttgc gcacccccct cacttccatc
      961 aaggcggggc tggggctggt tgagatgagc atgtcggagc gtatgggcac ggacgagcgg
     1021 cagctaatca cgaacatccg ccgcaacgtc gagcggctgg ggatattggt caacgacctg
     1081 ctgaccttca gcgaaataga gacgcgcacg ttccaactgg cgtgcaagcc gttggacctg
     1141 cgggcggttg tcgaagacgc ggtggcgatg atagagccac ttgtgcagaa gaaaaggcag
     1201 gtcgtagagg tagatatgcc ggaggccgtg ccctacaacg gggaccgcac gcggcttgag
     1261 caggtggtgg tgaacttgct gcataactcg cacaagtacg cgccggaggg ggcacgaatc
     1321 accgtgtcgg ggcaagttac gcccggcgag gtgctgctgg cggtgcgcga cgacgggccg
     1381 ggcatcccaa tcgaggagca cgaagcgata ttcgagcggt ttgatcacgg ccaccccgtc
     1441 aggagtggcg agggctgggg cctggggctg gcaatagcgc gctccatagt tgagatgcac
     1501 ggcgggcgca tctgggtaga aggccaacag ggagaaggcg caaccttcca tatagccctc
     1561 ccactccccg gcgagcctgc gatataactt tctataaaac aaataacaaa agctgtgtcg
     1621 gcgcggagcg ccgacacagc ttttgttatt tgttttaatc tcccctcccc acaggggag
//
LOCUS       DASRWZ010000645         6261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_257426, whole genome shotgun sequence.
ACCESSION   DASRWZ010000645 DASRWZ010000000
VERSION     DASRWZ010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257426"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..945
                     /locus_tag="VFH60_10220"
     CDS             193..945
                     /locus_tag="VFH60_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6314202.1"
                     /translation="MLVHAGIADHRMWDDQWDLYSRRYRVIRYDTRGYGGTPILDREY
                     SNRHDIYDLLSHLGVDKTYIVGVSRGGQIATDFTLEHPEMVDGLVLVGAGIGGFTEGP
                     EPTPDELRAFEEMEEAETKEDWELVAELDVRVWVDGFGRPDGRGDERVRDKVRQMCIE
                     TYRRPQGSGQPVVLDPPAAGRLDQVKVPTLVLVGEYDVSNAQVSADALATRIPGARKV
                     VIEGTAHVPNMERPEEFDRLVLDFLRGIEKAD"
     gene            1030..1323
                     /locus_tag="VFH60_10225"
     CDS             1030..1323
                     /locus_tag="VFH60_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314203.1"
                     /translation="MLTASPNTGTTNTRVNPERYVAMSDALLMVLPADGPGLTWPEIV
                     DAVAEKVPEGLFPEVKTVRWYSICVRLDLETKGLVERLQEKKRLRFRRVKTTI"
     gene            1382..1795
                     /locus_tag="VFH60_10230"
     CDS             1382..1795
                     /locus_tag="VFH60_10230"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HET6314204.1"
                     /translation="MPTTSTIHATGTFVVKSWDEKPYEELGEGAKLTRASVAETFQGD
                     IEGEGTVEYLMMYPDANTASYVGMQRVNGSLGGRTGSFVLQVSGTFAGGLAQATWSVV
                     PGSGTGELRSLRGEGGFPAQHGTSSVLYTLDYSFE"
     gene            complement(1848..2258)
                     /locus_tag="VFH60_10235"
     CDS             complement(1848..2258)
                     /locus_tag="VFH60_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET6314205.1"
                     /translation="MYHTSLFGRFAKLTARATGHPAAFAVALLTIIAWVVTGPIFGFS
                     DTWQLVINTGTTIVTFLMVFLIQNTQNRDSIAVHLKLDELIRAMSGAHNGLLDLEELT
                     EGELIKIRSTYEALARKAKQDLERGLIDTGTPNI"
     gene            2849..4048
                     /locus_tag="VFH60_10240"
     CDS             2849..4048
                     /locus_tag="VFH60_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314206.1"
                     /translation="MLQAQSNQMPSIADLLVRGKQAAMSGQRELARETLAHVVYLDPG
                     NAEGWLWLSGVVDQPEQVRYCLERTLRIEPHNSRALRGVEWLDSRQKEIDEARAAAER
                     VKPYWQTSKPKPLLNLIKGQSHAASVGTPVPLPRSGGQDQQQPASWASVGASIPNTRV
                     ARANMPTMPVSGETLDRLQRNPQAAPVNDRQYREEVLETDDELAPRFVTNIALHNLMK
                     AVDKVTGASGLAAMLRMAGLEELEGLDLEPDETPAINHTRFSAFGEAMEEFYTHAVDT
                     MEYKVGREMFRQELAMKGKMVAMKGITFKLMSPDKKLRSILMELADAHARMGMEAYFE
                     EREGGYLFGIETCPYCYGRLTNAHCNVTTGFLAAGIEWATGRALEIQEVSCRGIDEEF
                     CGYWIPV"
     gene            4226..>6261
                     /locus_tag="VFH60_10245"
     CDS             4226..>6261
                     /locus_tag="VFH60_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314207.1"
                     /translation="MHHRKPPTTAKRTFLPIFTALVLLVTSLIPASARQVNIATPQAA
                     EELPQPLWITPRGGEPLFVVGANYEGPVDRAWLMWEDDKFDIGLIEQDFSRASAFGIN
                     TLRIFVQKPLRDDINAGDFSKLDAVTSLARKHNLYLILTFTDWAEPDLSKAADLNGKI
                     AAHLVEEPSILAYDVKNEPQFSDVIGAIYSSVGLTVPLQVPDTIATYGERVTRAELAA
                     YRQSRERKAIIPARMTEDQAYSVANYYKLYLEFLDAGAAWVRQHPNTTTLDYLDSADS
                     IAWAPYLRALDETLGAWVSSQIATVRDADPARPVTTGYSNAVLAKMPSNRQLDFQSVH
                     RFTSHGYSGLNGTFLVLENLQRTFAGQPVMLEEFGYPGAVRNGSGGVTGYDPRTTANL
                     ESAVWTYLYSRGYAGGAKWMLNNFPQGDNPAENSFGLFDNDGKPKLQAYALRQLADLF
                     SRHEPGTFSDFRSEASSAVNFAYKAHDALVAGGKVYTSDNLTFQANVPSQLVVGTKGG
                     GVTLFATDVATTELHLPTVFGIATADLGRVTLTGLDPAGRPSMPSQPMLSGDWLRINL
                     APLYNYRLTVVPRAVEHAPAQADPNTVYFPQTGHNLTGEFLKYWQAHGGLSIFGYPLT
                     EAFTEGGYTVQYFERNRFELHPENQPPYNVLLGRLGADLTTGRTFDSVQPFEPSP"
BASE COUNT         1258 a         2037 c         1886 g         1080 t
ORIGIN      
        1 accgcgccca tatcgcgacc atcctcaccc agcagggcgt aactcctccc gaaatcgacg
       61 gttggagcta catgatggcg atgaaagcgg aaggagtcac gactgtctga cgttaccagc
      121 ggtccgtcag gaacgattga ggcagcgggc ggcccccttt actacgaggt cgcgggggag
      181 gggcacccgc tcttgctcgt ccacgcgggc atcgcggacc accggatgtg ggacgaccag
      241 tgggacttgt attccaggcg ctaccgggtg atccgctacg acacgcgcgg ctacggcggc
      301 actcccatac tcgaccgcga gtacagcaac cgccatgaca tttacgacct gctctcccac
      361 ctgggcgtgg acaagaccta catcgtgggc gtatcgcgtg gcgggcaaat agccaccgac
      421 ttcacgttgg agcaccctga gatggtggac ggtctcgtgc tcgtgggcgc gggcatcggc
      481 ggcttcacgg agggacctga gccgacaccg gatgagctgc gggcgttcga ggagatggag
      541 gaggccgaga cgaaggaaga ttgggagctt gtggccgaac tcgacgtgag ggtgtgggtg
      601 gatggcttcg gccggcccga tgggcgaggc gacgaacggg tgcgtgacaa agtgcgccag
      661 atgtgcatcg agacctaccg gcggccccag ggaagcggcc agcccgtggt gctggaccct
      721 ccggcggcgg ggcggctcga tcaggtcaag gtgcccacgc tcgtcctggt gggcgagtac
      781 gacgtctcta acgcccaggt aagcgccgat gccctggcca cccgcatccc cggcgcgcga
      841 aaggtggtta ttgaaggcac ggcccatgtg cccaacatgg agcggcctga ggagttcgac
      901 aggctggtgc tcgacttcct gcgtggcatc gaaaaagctg attgaaagac aagttgttaa
      961 caagcgaaat tagccaataa gccacgaaaa ggacaatttc actgctttgt caggcacagg
     1021 aggtagaaaa tgctgaccgc aagcccaaat accggcacaa ccaacacccg cgtgaacccg
     1081 gagcggtatg tagccatgtc tgatgcgctg ctgatggttt tacctgctga cggccccgga
     1141 cttacctggc ctgagatcgt ggacgccgtt gccgagaagg tgccggaggg actcttccca
     1201 gaagtcaaga cggtacgttg gtactccatc tgcgtgcgcc tggaccttga aacgaaaggt
     1261 ctcgtggagc ggctccagga gaagaaacgc ctgcgcttcc ggcgggtaaa gaccacaata
     1321 tagactcagg ttgaagccag cctgtgtttt acacgaacaa tcggaggtac gaaacttagt
     1381 tatgccgacc acatccacaa tccatgctac aggcacattc gtggtaaaga gctgggacga
     1441 aaagccctat gaagaactgg gcgagggggc caagctcacc cgtgccagtg tggccgagac
     1501 cttccagggg gacatagagg gagaaggtac ggtagagtac ctgatgatgt accccgatgc
     1561 gaacaccgcc agctacgtcg gcatgcagcg cgtgaacggc agcctgggcg ggcgtacggg
     1621 cagcttcgtg ctccaggtga gtggcacgtt cgcgggtggg ttagcgcagg ccacctggtc
     1681 cgtggtgccg ggttcgggga cgggggagtt gcgcagccta cgaggcgaag gcggcttccc
     1741 ggcccagcac ggcacgagca gcgtgctcta tacgctagac tacagcttcg agtagcatag
     1801 ccagtgtacg tgccgagtcc accgctcggc attcgcggtc ggcctatcta tatattaggc
     1861 gtcccggtgt ctatcaggcc ccgttccagg tcttgcttcg ccttgcgagc cagcgcttcg
     1921 taagtgctcc gtatcttaat cagctcaccc tcggtcaact cctcaaggtc cagcagcccg
     1981 ttgtgagcgc cggacatcgc tcgtatcaac tcatcaagct tcaggtgcac cgctatgctg
     2041 tccctgttct gggtattctg gatcaggaat accatgagga aggtcacaat agtagtcccg
     2101 gtgttgatca caagctgcca ggtatcgctg aagccgaaga tcggccctgt aaccacccag
     2161 gcgataatcg tcagcaacgc cacagcgaat gccgccgggt gcccggttgc gcgggccgtc
     2221 aatttggcaa agcgtccgaa caaagatgta tggtacatag ttttcctcct gggtaatgtc
     2281 ttaaaagtca cccaagcttt cagcacaagc acctgttaag caggtgcttc actggtgaga
     2341 cgttcaacca acagcctcgc tacagcacgc accttgtttc agcgcggcgc aaacttctcc
     2401 aattcctcca tcccgcaagt cagacgaggc cctcatagac ctcactccgc accccatcgc
     2461 tcaacaattg cgacatcctg cttctaaagc ttagcaccag cagtaggttc gattccacct
     2521 ttccactttc cttccttcgc cccgcctgtc gagccacacc ggcaggtacg attgtggaaa
     2581 actcaccggc cggtgcttgc cagttttacg cgtgattcgc gcctcaaggc ggcgtctgca
     2641 cagcacgtcc accgcctttc cccacgttat gcgcataatc cgcacaagtc cgcatccctg
     2701 tgctaatata gctttgtaac gaccaaacgg gcacacaaag cacgcggcgt tacacagacc
     2761 aggtggagcg gcaaggtgcg cggtggggct tgcgggcgta gctaatgcgt cgaccacagg
     2821 cccggagcta gaatagggga gcacggagat gttacaggcc caatcgaacc agatgccttc
     2881 tatagccgac ctgctggtgc ggggcaagca ggcagccatg agcggacagc gcgagctggc
     2941 ccgcgagacg ctggcccacg tggtctacct cgacccgggc aacgccgagg gctggctctg
     3001 gctttcaggc gtggtggacc agccggagca ggtgcgttac tgtttggaac gcactctgcg
     3061 catcgagccg cacaactcgc gagcgttgcg cggtgtcgag tggctggact cgcgccagaa
     3121 ggagatagac gaggcccgtg ccgccgccga gcgagtgaaa ccctactggc agacctccaa
     3181 gcccaagccg ctcctcaacc tgatcaaagg ccaatcccac gccgcctcgg tgggcacccc
     3241 cgtgcccctg ccccgttcgg gcggccagga ccagcagcag cccgcaagct gggcctccgt
     3301 cggcgcgagc atccccaaca cgagggtagc ccgcgccaac atgcccacca tgcccgtgtc
     3361 cggcgagacg ctggaccgct tgcagcgcaa cccccaggcc gcgcccgtca acgaccgcca
     3421 gtaccgcgaa gaggtgctgg agaccgatga cgagctggcc ccgcgcttcg tcaccaacat
     3481 cgctcttcac aacctgatga aagcggtgga caaggtgacg ggtgccagcg gcctggcggc
     3541 gatgctgcgc atggcaggct tggaggagct tgagggcctc gacctcgaac ccgacgagac
     3601 gcccgcgatc aaccacactc gcttttccgc tttcggtgag gcgatggagg agttctacac
     3661 tcacgcggtc gacacgatgg agtacaaggt gggccgcgag atgttccggc aggagcttgc
     3721 catgaagggc aagatggtgg ctatgaaagg catcaccttc aagctcatgt cgcccgacaa
     3781 gaagctgcgt tccatactga tggagcttgc cgacgcgcac gctcgtatgg gtatggaagc
     3841 ctacttcgag gagcgcgagg gtggctacct gttcgggata gagacctgcc cctactgcta
     3901 cggtcgcctc accaacgccc actgcaacgt taccaccggc ttcctggccg cgggtatcga
     3961 gtgggccacg ggccgggcgc tcgaaataca ggaggtgtcc tgccggggga ttgacgaaga
     4021 gttctgcggc tactggatac ccgtgtagag gcagcaaggc cgggcgcgcc gaggactggg
     4081 aggggagcat gacctgcttc gctcccagtc cttgccgtct gtgcggcttg tgcaaccgct
     4141 tgcctgtagg cgcgttattc tcacaagcgg cacagctatg tagaaccggt aacataaaca
     4201 ggctcatacg cacagggtat cgctcatgca ccacagaaaa cccccaacca ccgcaaagcg
     4261 cacgttcctt cccatcttca ccgcgctcgt gctacttgtc acgagcctta tacccgcctc
     4321 cgcgcggcag gtgaacattg ccacacccca ggccgccgag gagttgccgc agcccctatg
     4381 gatcaccccg cgtggcggcg agccgctgtt tgtggtggga gctaattacg aggggccggt
     4441 ggatcgtgcc tggctgatgt gggaggacga caagttcgac atcgggctga tcgagcagga
     4501 cttctcgcgc gccagcgcct ttggcatcaa caccctgcgc atcttcgtcc agaagcccct
     4561 gcgcgacgac atcaacgccg gtgacttctc caagctggac gcggtcacct ccctcgcccg
     4621 caagcacaac ctctacctga tccttacctt cactgactgg gccgaacccg acctctcaaa
     4681 ggccgccgac ctgaacggca agatagccgc gcacctggtg gaggagcctt caatcctggc
     4741 ctacgacgtg aagaacgagc cgcagttcag cgatgttatc ggcgcgatct actcgagcgt
     4801 tggcctcacc gtgcccttgc aggtgcccga tacgattgcg acgtatgggg agcgcgtgac
     4861 gagggccgag cttgccgcct acaggcaaag cagagagcgt aaggccatca tcccagcgcg
     4921 catgacagag gaccaggctt attccgtcgc caactactac aagctctacc tggagttcct
     4981 cgacgccggg gccgcctggg tgcgacagca cccgaacacc accacgctgg actacctcga
     5041 ctcggccgac tctatcgcct gggcacccta cctgcgcgcc ctggacgaga cgcttggggc
     5101 gtgggtgtcg tcgcaaatcg ccaccgtgcg cgacgccgac cccgccaggc ccgtcacgac
     5161 cggctacagc aacgcagtgc ttgccaagat gccctcaaac cgccagctgg acttccagtc
     5221 ggtgcaccgc ttcacctcgc acggctactc cggcctgaac ggcaccttcc tcgtgcttga
     5281 gaacttgcag cgcaccttcg cggggcagcc ggtgatgctg gaagagttcg gctatccggg
     5341 ggcggtgcgc aatggcagcg gcggcgtcac cggctacgac ccccgcacca ccgccaacct
     5401 ggagagcgcg gtgtggacct acctctacag ccggggctac gcgggcgggg ccaagtggat
     5461 gctcaacaac ttcccccaag gggacaatcc cgccgagaac tcgttcggcc tgttcgacaa
     5521 cgacggcaag cccaagctcc aggcctacgc gttgcgccag cttgccgacc tgttcagccg
     5581 ccacgagccg ggcaccttct cggacttccg ctccgaggcg tcatctgccg tcaacttcgc
     5641 ctacaaggct cacgacgcgc tcgtggcggg cggcaaggtc tacaccagcg acaacctgac
     5701 cttccaggct aacgtgccct cgcagctcgt ggtgggcacg aagggcggcg gagtcaccct
     5761 gttcgccacc gacgttgcca ccaccgagct acacctgcct accgtcttcg gcatcgcgac
     5821 cgcggacctc ggcagggtga ccctcaccgg cctcgacccc gccgggcggc cctctatgcc
     5881 ctcccagccc atgctgagcg gcgactggct caggatcaac ctggcaccgc tctataacta
     5941 ccgcctgact gtggtgccca gggccgtgga gcacgccccc gcgcaggccg accccaacac
     6001 ggtgtacttc ccgcagaccg gccacaacct cactggcgag ttcctcaagt actggcaggc
     6061 gcacggcggc ctctccatct tcggctaccc cctgaccgag gcgttcaccg agggcggcta
     6121 cacggtgcaa tacttcgagc gcaatcgctt cgagctacac cccgagaacc agccgccata
     6181 caacgtcctc ctgggcaggc tgggggccga cctgaccaca ggccgcacct tcgacagcgt
     6241 tcagcccttc gagccgtccc c
//
LOCUS       DASRWZ010000646         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_257803, whole genome shotgun sequence.
ACCESSION   DASRWZ010000646 DASRWZ010000000
VERSION     DASRWZ010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257803"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFH60_10250"
     CDS             complement(<1..737)
                     /locus_tag="VFH60_10250"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET6314208.1"
                     /translation="MTSTQPERNELAIGRAATLKSIYEIAGMMGLEHGDIEPYGSTKA
                     KIRLEVIERNRDRPNAKYVVVTAITPTPLGEGKTTTSIGLAQGMYHIGKHAIVALRQP
                     SLGPVFGIKGGGSGGGYSQVVPMEDMNLHLTGDIHAVAVAHNLLSAFLDASIFHGNPL
                     NIDIERIELRHVMDMLDRSLREINKGLGGKNNGIPRQSGFDIAVASEAMAILALCKDI
                     FDLRERLGRITVAQDAEGKPVTAEQLRA"
     gene            complement(769..1683)
                     /locus_tag="VFH60_10255"
     CDS             complement(769..1683)
                     /locus_tag="VFH60_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HET6314209.1"
                     /translation="MPEVESTQMPPHDEISAPQSPLLLDGRAVAGEMRAQYACEAAAL
                     KARFGLLPGLGVLRVGNDPASVSYTGRISKAFAEAGIAVTINELPLSASRAMLRAELG
                     RLNVLPEIAGIIVQMPLPAHIGLDALVDVLDPDKDVDGLHPANVGSLTLGLEAYVPAT
                     PAGGLALLDYYGIKLEGLNALVVGRSGVVGRPLAQLLLARNATVTIAHTHTRDLEDLI
                     SRADLVATAVGKPGLIRGHCIKPGAVVLDFGASIVEGHMTGDVQFEEAALRASAITPV
                     PGGTGPMTNAMLLRNTLNAIKRYFGVTS"
     gene            complement(1738..2478)
                     /locus_tag="VFH60_10260"
     CDS             complement(1738..2478)
                     /locus_tag="VFH60_10260"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6314210.1"
                     /translation="MSAMTQSLENTKLVISPGSNQTLVFLVRHAQTEWNLERRFQGQF
                     DVPLSATGLQQAVIVADWLKNQLQHFAALYTSDLARARQTAQPIGERLGLVPQPVEAL
                     REIHAGDWQGLLTVEIEERYPGQMLRWHREPHDFRLPGGETIPEVQARVLAWYREAVA
                     AHRGRAIVAVSHGLAIRTLLAALNGWDVADAEAMKTAGLGNTGVAVMLADHGTGRSTM
                     LLLNYLGHLDEASSLGAAGERTNEPTAV"
     gene            complement(2475..>2681)
                     /locus_tag="VFH60_10265"
     CDS             complement(2475..>2681)
                     /locus_tag="VFH60_10265"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6314211.1"
                     /translation="YRGRGIAEALVRHTLQYLASVGMTSATLFVESQNTAARRLYTKM
                     GWRPVYRTDHYWRRMPLSTEPQTI"
BASE COUNT          424 a          828 c          895 g          534 t
ORIGIN      
        1 gcccttagct gctcggcagt gacgggctta ccctcggcgt cctgggcgac ggtgatccta
       61 cccagccgct cgcgcaggtc gaaaatgtcc ttgcaaagcg cgaggatcgc cattgcttcg
      121 gaagctacgg ctatgtcgaa gccgctttgc cgtggtatgc cgttgttctt gccccccagg
      181 cccttgttga tttcgcgcaa ggagcgatcg agcatgtcca tgacgtggcg cagctctatg
      241 cgctcgatgt cgatgttgag ggggttgccg tggaagatgg aggcgtcgag gaaggccgag
      301 agcaggttgt gagcgacggc tacggcgtgg atgtcgccgg tgaggtggag gttcatgtcc
      361 tccatcggca ccacctggct gtagccgccc cccgaaccgc cccccttgat gccgaatacg
      421 gggccgagcg acggctgccg cagggccaca atggcgtgct tgccgatgtg gtacatgccc
      481 tgcgccaggc cgatagaggt ggtcgtctta ccctcgccca gcggggtggg ggtgatggcg
      541 gtgacgacga cgtacttggc gtttggcctg tcgcggttgc gctctatcac ttcgagcctg
      601 atcttggcct tggtgctgcc gtatggctcg atgtccccgt gctcgaggcc catcataccg
      661 gctatctcat atatcgactt gagcgtggcc gctcgaccta tggcaagctc gttgcgctcc
      721 ggttgtgtgg aggtcatctg gtccttccag gtgaaagtat gggcgtcatt atgaggtaac
      781 gccgaagtaa cgctttatcg cgttcaacgt gttgcgcagc agcatggcgt tggtcatagg
      841 gccggttccg ccgggtacgg gggtaatcgc ggaagcgcgc aaagcggcct cctcgaactg
      901 cacgtccccc gtcatgtgcc cttcaactat gctggccccg aaatcgagca ccaccgcgcc
      961 cggcttgatg cagtgcccgc gaatcaaccc cggcttgccg acggcggttg ccaccaggtc
     1021 ggcccgcgag atgaggtctt cgaggtcccg cgtgtgggtg tgggcgattg tcacagtggc
     1081 gttacgggca agcaggagtt gggccagcgg cctgcccaca acgccgctac ggcccaccac
     1141 gagggcgttc aggccctcca gcttgatgcc gtagtagtcg aggagggcca gcccaccggc
     1201 gggggtagca ggcacgtaag cctcaaggcc cagcgtcagg ctgcccacgt ttgccgggtg
     1261 caggccgtcc acgtccttgt cggggtcgag cacgtccacg agggcgtcga ggccgatgtg
     1321 ggctggcagg ggcatctgca cgatgatgcc cgctatctca ggcaggacgt tcaagcgccc
     1381 tagctcggcc cggagcatgg ccctcgacgc gctcaggggt agctcgttga tggtgacggc
     1441 gatgcccgct tccgcgaagg ccttgctgat gcgcccggtg tagctgacgg aggcggggtc
     1501 gttccccacg cgtagcacgc cgaggcccgg cagtagtccg aaccttgcct tgagagcggc
     1561 ggcttcgcag gcgtactggg cgcgcatctc ccccgcgacc gcgcgtccgt cgaggaggag
     1621 gggcgattgg ggcgcgctta tctcgtcgtg cgggggcatc tgcgtggatt caacttccgg
     1681 catagtaata gtcatcgcac atcctggctt tcgtgtctaa ccgcgtcgca tcttccatca
     1741 tacggctgtc ggttcgttgg tgcgctcccc ggcggccccc aggctcgaag cctcgtccag
     1801 gtggcccagg taattgagaa gcaacatcgt gctgcggccg gtgccgtgat cggccagcat
     1861 taccgccacg ccagtgttcc ccaggcccgc ggtcttcata gcttcagcgt cggccacgtc
     1921 ccagccgttg agcgcggcca gcagggtgcg tatcgccagc ccatgggaca cggccacgat
     1981 ggcccggccc cggtgcgcgg cgaccgcttc ccggtaccag gcgaggacgc gggcctgcac
     2041 ttcgggtatg gtctcgccgc cgggcaaccg aaagtcgtgt ggctcccggt gccagcgtag
     2101 catctggccg gggtaacgtt cctctatttc cacagtcagc aggccctgcc agtcgcccgc
     2161 atgtatttcg cgcaaggctt ccacgggttg gggcaccagg ccgaggcgtt cgccaatagg
     2221 ctgagccgtt tgccgggcgc gcgcaaggtc actagtgtag agagcggcaa agtgttgaag
     2281 ttggttcttt agccaatcag ccacaatcac cgcttgttgc aggcccgtgg cgctaagcgg
     2341 cacgtcaaac tggccctgga agcggcgttc caggttccat tccgtctgcg cgtgtctcac
     2401 caggaagacc agagtctggt tgctgccggg cgagattacg agcttggtat tctcaaggct
     2461 ttgagtcatg gcgctcatat cgtttgtggc tcggtggaca ggggcatgcg cctccagtag
     2521 tggtcggtgc ggtagacggg ccgccaaccc atcttagtat agagccttcg cgcggcggtg
     2581 ttctggctct ccacaaagag cgtggcgcta gtcatgccga cggatgccag gtattgaagc
     2641 gtgtgcctca ccagggcctc agctatgccc ctcccgcgat a
//
LOCUS       DASRWZ010000647         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1217822, whole genome shotgun sequence.
ACCESSION   DASRWZ010000647 DASRWZ010000000
VERSION     DASRWZ010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217822"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1575)
                     /locus_tag="VFH60_10270"
     CDS             complement(139..1575)
                     /locus_tag="VFH60_10270"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET6314212.1"
                     /translation="MIPEQAIDRALVEATRLGAEYAEARAETDTSERVHARNGDVERL
                     GSDTDSGWGIHVLAGGGWGFASTSSEAIQDIRDTVQRAVEIARASGTRHKSRSDLDIM
                     PTWQGEYETNLQQDPFEVPLRERVALAQETTHRMLAAGDRLKVATCTLYAQRAVKLYA
                     NTRGARLRQRITYVGIDLRATATHEGYSYRRSLDDESQAGWEFVRRLDPPGQGERVGR
                     EAAELVDAPWAPSGPRTVILSSDMVALLVHESCGHPTELDRVLGWEAAFAGTSFLMPD
                     MLGTFRYGSPLVNMTADSLLPGGLGTFGWDEEGTPAQRSPIVREGIFTGYLTSRESAL
                     DIGQRSNGCGRAISWGRIPIVRMVNLSLEPGGGSLDDLVSQVDDGLYLETPSSWSLDD
                     KRMNFHFSAELCREIKGGKLGELRKGASFQGRTPAFWGSVRGIAGPEEWHLWGFPHCA
                     KGEPLQLCPVSHGASPIRVEGLQMSGHS"
     gene            complement(1635..2288)
                     /locus_tag="VFH60_10275"
     CDS             complement(1635..2288)
                     /locus_tag="VFH60_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6314213.1"
                     /translation="MSSREKKLSYEWQPSRGWYVVIRAFFTVVFHTIWPLRVSGRENV
                     PRKGAAIIVCNHLSLVDPFVVGYAANRIVNFMGKEELFSVPVVGFLIRKVGAFPVDRS
                     RRDPVAMRTALQVLKSGELLGMFPEGTRSTTGEMLEFRAGAARLAARTEVPIIPAAVH
                     NTGKAMPPGKLLRPARISIRFGEPFELTELYDRKDKGEAMDRALQTIKERIEALAEP"
     gene            complement(2378..>3313)
                     /locus_tag="VFH60_10280"
     CDS             complement(2378..>3313)
                     /locus_tag="VFH60_10280"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET6314214.1"
                     /translation="NWTGGRGARLKGLGVRAASGAVLLAVVIAAIWLQGPLLMLCIAV
                     AAGFAAHEFYGIVRKAGYKPWYLAGVSLALLLALRGYPAGSLLSGVPYVGPSVAAEVL
                     VLVLALFFALARQGLYRRVGLLAARAEGSPGETAGAPGRSPYLTWSDLGLTLAGALYA
                     GGLLGYAPLLAAEADRTHAGSAFTWLLLVLLGTAACDTGAYFAGSLLGRHKLIPHISP
                     AKTWEGLAGGTLGAVAAALAFSGPLGLSLPMAVVLGLVVCAAAVSGDLCESLLKRAAG
                     VKDSGRIIPGHGGVLDRIDSILFVLPAVYWFAQVT"
BASE COUNT          606 a         1123 c         1043 g          541 t
ORIGIN      
        1 ggatggagaa cgcctcagcc tcccctacca gaccccagtt tccaccgcta cctgtcatgt
       61 gccatgtcct tccgaaagga tcgcgtgtcg ataaagtgcc agcaacgccg gtagcgcgcc
      121 gtcagataca gaatggacct agctatgccc gctcatttgc aggccttcca cgcggatggg
      181 cgaggccccg tgcgacaccg ggcacagttg cagaggctca cccttggcgc aatgtgggaa
      241 gccccacagg tgccactcct caggccccgc gatgccccgc accgaccccc agaaagcggg
      301 cgtgcggccc tggaacgacg cgcccttgcg cagctcgccc aatttgccgc ccttgatttc
      361 gcggcacagc tccgccgaaa agtggaagtt catccgcttg tcatccaggc tccacgagga
      421 aggcgtctcc aggtaaaggc cgtcgtccac ttgcgaaacg aggtcgtcca gcgagccgcc
      481 acccggctcc agcgaaaggt ttaccatccg cactatcggt atgcggcccc aagagatcgc
      541 ccgcccgcag ccgttggaac gctggccgat gtctaaggcg ctctcacgcg aggtgagata
      601 gccggtgaat atgccctcgc gcacgatggg gctgcgctgg gcgggggtgc cctcttcgtc
      661 ccaaccgaag gtgcctagcc cgccgggcag cagcgaatcg gcggtcatgt tgaccagggg
      721 cgagccatag cggaaggtgc ccagcatgtc aggcatgagg aacgaagtgc ccgcgaaggc
      781 tgcctcccac ccgagcacgc ggtcaagctc ggtagggtgg ccgcacgact cgtgcaccag
      841 cagcgcgacc atgtccgaag aaaggatgac cgtgcgcggc cccgagggcg cccagggcgc
      901 atcgactagc tcggctgcct cccgccccac gcgctcgccc tggcccggcg ggtcgagccg
      961 ccgtacgaac tcccagcccg cctggctctc gtcatccagc gacctgcgat acgaatagcc
     1021 ttcgtgagtg gcggtcgccc ggaggtcgat gccaacgtag gtgatacgct ggcgcaaccg
     1081 cgctcccctg gtgttggcat aaagcttcac tgcgcgctgg gcgtaaaggg tgcaggtggc
     1141 taccttcagg cggtcgcccg ccgcgagcat acggtgagtg gtctcctgcg ccagggccac
     1201 tcgctcccgc agcggcacct cgaaagggtc ttgctgtagg ttggtctcgt actcgccttg
     1261 ccaagttggc atgatatcga ggtccgagcg ggacttgtgg cgggtgccgc tggccctggc
     1321 gatttcgacg gcccgctgta ctgtgtcccg tatatcctgg atggcttcgg acgaggtcga
     1381 agcgaagccc cagccgccgc ccgcaagcac gtggatgccc cacccgctgt cggtgtccga
     1441 accaagccgc tccacgtcgc cgttgcgggc gtgcacccgc tcggaggtgt cagtctcggc
     1501 ccgcgcctcg gcatactcgg cccccaggcg cgtggcttct accagcgcac gatcgatagc
     1561 ttgctcagga atcatggggc attatagcac agggctttgt ggcgagtggc cggtggtcag
     1621 ggtgtaaaca cttatcaggg ctcggccaac gcttcgatgc gctcctttat ggtctggagc
     1681 gcgcggtcca tggcttcgcc cttgtctttg cggtcgtata gctctgtaag ctcgaacggc
     1741 tcgccaaagc ggattgagat gcgggcgggg cgcaggagct taccgggagg catcgccttg
     1801 ccggtgttgt gcacggcggc cgggattatg ggcacctccg tgcgggcggc caggcgggcg
     1861 gcccccgctc ggaactccaa catctccccg gtagtgctgc gggtgccctc cgggaacata
     1921 cccagcagct cgcccgattt tagcacctgt aaggccgtgc gcatggctac ggggtcgcgc
     1981 ctggagcggt ctacaggaaa agcccccacc ttgcggatga ggaaccccac gacgggcacg
     2041 ctgaacagtt cttccttgcc catgaagttc acgatgcggt tggcggcata gccgaccacg
     2101 aacgggtcta cgaggctcag gtgattgcac acgattatgg cggcaccctt gcgcggcacg
     2161 ttttctcgcc cgctcacccg caacggccag atagtgtgaa acaccacggt gaagaaggcc
     2221 cggatcacca cgtaccaacc ccggctgggc tgccattcgt agctaagttt cttctcacgc
     2281 gaggacatgg gcactcctgc gaatttggga attggaattt cagatttcga atttgttgga
     2341 tacctggtaa attcgaaatc cgaaattcca aatccggcta tgtgacttgc gcgaaccagt
     2401 agactgcggg gagcacgaac aggatgctgt cgatgcggtc gagcacaccg ccgtggcctg
     2461 ggattatgcg gccgctgtcc tttacgcctg ccgcgcgctt caacagggac tcgcagaggt
     2521 ccccggagac cgcggcggcg cacaccacca gccccagcac gactgccata ggtaggctca
     2581 ggcccagcgg gccggaaaaa gcgagggccg cagccaccgc cccaagcgtg ccccccgcga
     2641 ggccctccca ggtcttagca gggctgatgt gtggtatcag tttgtgcctg ccaaggaggc
     2701 tccctgcgaa gtacgccccc gtgtcgcagg ccgcggtgcc cagcagcacc aagagcagcc
     2761 aggtgaacgc gctgcccgca tgcgtgcggt cggcctcggc ggccagcaac ggagcgtaac
     2821 ccagcagtcc accggcgtac agcgccccgg ccagagtaag gcccaggtcg gaccatgtaa
     2881 ggtaggggct gcgccccggt gctcccgccg tctcgccggg gctgccctcc gcgcgggcgg
     2941 cgagcaggcc cacccgccgg tacaaccctt gccgggccag tgcgaagaac agcgccagca
     3001 ccaaaacaag cacctcggcg gctacgctcg gcccgacata ggggacaccg gacaataagc
     3061 tacctgcagg gtagccgcgc agggccagca gcagcgcgag cgacacgccg gcgaggtacc
     3121 agggcttata acccgccttg cgcactatac cgtagaactc gtgcgcggca aacccggcgg
     3181 ctacggcgat gcacagcatc agcagcggac cttgcagcca tatggccgca atcaccacgg
     3241 caagcaacac cgccccgctg gcagcccgca cccccaggcc cttcaaacgc gctccccttc
     3301 cgccggtcca att
//
LOCUS       DASRWZ010000648         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1149289, whole genome shotgun sequence.
ACCESSION   DASRWZ010000648 DASRWZ010000000
VERSION     DASRWZ010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149289"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VFH60_10285"
     CDS             <1..1137
                     /locus_tag="VFH60_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314215.1"
                     /translation="KRNAETGIKESPTVIWTRPRGLHISQGGVFRDREELTSASLFDV
                     AMIVYQADPARLKHPLCFYIPKSESADEALWWRDLFQAISAAKGWPRDYIKCMALVES
                     HPLAFQMEEFVYNLREHILGLNLGRWDYMASLIHFNFNDPNWVLPDRNTIPHNVPFFQ
                     NLRELIPEICHKRGILAIGGMTALYPSRVDPELNERALKVLAEDKKNEANSLMDGAWT
                     GHPDQNEIAVSQFPYPNQLDARPDGIERYPDLRPTPAGVGKRTLAGTRAAVRTVIRYR
                     NGVLNGKGASLLDGYMEDLATDRIYRLMIAQRMKHSATVPISAEAGHAVEHTPELVSR
                     LFDEELDRLLGELPQDAPPGAAGKFRQAREISERMISRGEFDPV"
     gene            1435..2649
                     /locus_tag="VFH60_10290"
     CDS             1435..2649
                     /locus_tag="VFH60_10290"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HET6314216.1"
                     /translation="MVHEVPTNVTQDGHSNGHGNGQNPAPAQPQVELAPTALLPEGYM
                     VPKSRWTHPSTRMRQLLESEPYLFGPGVYDPFGAQLVMYYGFKAVYFSGYSFAIGHLG
                     TTDMDLYSSVEIADAARRTVSAMRKFQLTMAIGDPEKGVPPKHLEIPPLIVDMDGGYG
                     NIFNVQRTAELYVGAGVAAAHIEDQVLPKRCGHIGGKALIGVNEMVGKLRMARAVADD
                     MGNSDFVIIARTDGLSAVDAPESQRGMDLAIERGLRYLDTGIPDLLWCEFPTSDREPV
                     ETFASEIKKRFPDARFAFNWSSSFKWFNDPNPTTFAQLGEMGFKFLFITLGGQHATGY
                     GLSNLLQEMSQTQEQGYINLQRKEFEGGDYPTKSHHFFSGVPYHHAVGKLYDAARLGA
                     EYLEDIPEGKVV"
BASE COUNT          582 a          829 c          854 g          461 t
ORIGIN      
        1 aagcgcaacg ccgagacggg cataaaagag agccccacgg tcatctggac gaggccgcgc
       61 ggcctccaca tttcccaggg tggagtgttc cgagatagag aagaactcac ctcggcttcg
      121 ctgttcgacg tagctatgat agtgtaccag gcggacccgg cccggctcaa gcacccgctc
      181 tgtttctaca tccccaagag cgagtcggcg gacgaagccc tctggtggcg cgacctcttc
      241 caggccattt cggcggcgaa gggctggccc cgcgactaca tcaagtgcat ggccctggtc
      301 gagtcgcacc ccctggcctt ccagatggag gagttcgtat acaacctgcg cgagcacatc
      361 ctgggtctca acctggggcg ctgggactac atggcgagcc tcatccactt caacttcaac
      421 gaccccaact gggtgctgcc cgaccgcaac acgataccgc acaacgtgcc tttcttccag
      481 aacctgcgcg agctaatccc ggaaatatgc cacaagcgcg gcatcctcgc tatcggcggc
      541 atgacggccc tctacccgag ccgcgttgac cctgagttga acgagcgcgc gctcaaggtg
      601 ctggccgagg acaagaagaa cgaagcgaac agcctcatgg acggggcgtg gacgggtcac
      661 ccagaccaga acgagatcgc ggtgagccag ttcccttacc ccaaccagct agacgcccgg
      721 cccgacggca tcgagcggta cccggacctg cgccccacgc ccgcaggtgt tggcaagcgc
      781 accctggcgg gcacccgcgc cgccgtgagg accgtcatcc gctaccgaaa cggcgtgctg
      841 aatggcaagg gcgcaagtct gctggatggc tacatggagg acctcgcgac tgaccgcatc
      901 taccggctga tgatagccca gcgcatgaag cacagcgcca cggtgcccat cagtgccgag
      961 gcgggtcatg cagtcgagca cacgcccgaa ctggtgagcc gcctctttga tgaggagctt
     1021 gatcgcctcc tgggggagtt gccccaggac gcaccgccgg gtgccgccgg caagttcagg
     1081 caggcccgcg agattagcga aaggatgatt agcaggggcg agttcgaccc ggtgtagtag
     1141 ttgttaagca gtgagaagta gccgggtgag agggacgaag ccccccttat gtcattctga
     1201 acgtagtgaa gaatctcaag aaggcagtca tggatgctgt tgaatgtcac ggacactgtc
     1261 atggacatag tactcatatt caatgactgt catggacata gtactcatct tcaatgtagg
     1321 gacacaagca gcatctgaga ttcttcacta cgttcagaat gacaggggac gtagcccagt
     1381 cgcgcaatgt tgcggctcga taatggaaag gttaacaagg aggttaacag catcatggtc
     1441 catgaagtac ccacgaacgt gacgcaggac ggacacagca acggacatgg caacgggcag
     1501 aaccccgcgc ccgcccaacc ccaggtggag cttgcgccca cggccctgtt gccggaaggc
     1561 tacatggtgc ccaagtcgcg ttggacgcac ccaagcaccc gcatgcggca gttgctggag
     1621 agcgagccgt acctgttcgg ccccggcgtg tacgacccgt tcggcgcgca gcttgtgatg
     1681 tattacggct tcaaggcggt ctacttcagt ggctattcgt tcgcaatagg ccacctcggc
     1741 accaccgaca tggaccttta ttccagcgta gagatagccg acgccgcccg ccgcacggtg
     1801 agcgctatgc gcaagttcca gcttacgatg gcgataggcg atcccgaaaa gggcgtgccg
     1861 ccgaagcacc tggaaatacc gcccctcatc gtggacatgg atggcgggta tggcaacatc
     1921 ttcaacgtgc agcgcacggc cgagctttac gtgggcgcgg gcgtggcggc ggcccacatc
     1981 gaggaccagg tgctgcccaa gcggtgcggc cacatagggg gaaaggccct catcggcgtg
     2041 aatgagatgg tgggcaagct gcgcatggcg cgggcggtgg ccgacgacat ggggaacagt
     2101 gatttcgtga ttatcgcgcg caccgacggc ctgagcgcgg tagacgcacc cgaatcgcag
     2161 cggggcatgg acctggcgat tgagcgtggc ctgcgctacc tcgataccgg cattcccgac
     2221 ctgctctggt gcgagttccc cacctcggac cgcgagccgg tggagacgtt cgcaagcgag
     2281 atcaagaagc ggttcccgga tgcgcggttc gccttcaact ggtcgtcctc cttcaagtgg
     2341 ttcaacgacc ccaacccgac gaccttcgcg cagttgggcg agatgggctt caagttcctt
     2401 ttcatcacgc tgggcggaca gcacgccacc ggctacggac tgagcaacct gctgcaagag
     2461 atgtcgcaga ctcaggagca gggctatatc aacctccagc gcaaggagtt cgagggcggc
     2521 gattatccca cgaagagcca ccacttcttc tccggcgtgc cctaccacca cgcggtaggc
     2581 aaactatacg atgcggcccg gctgggggct gagtacctgg aggatatacc agaggggaag
     2641 gtggtctagg ggaggacaga cgtcaggtgc tgggctaggg acggcggtct gtgggtgtgc
     2701 ccagcatgac acgcagcatg aggccc
//
LOCUS       DASRWZ010000649         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_875713, whole genome shotgun sequence.
ACCESSION   DASRWZ010000649 DASRWZ010000000
VERSION     DASRWZ010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875713"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            560..2032
                     /locus_tag="VFH60_10295"
     CDS             560..2032
                     /locus_tag="VFH60_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314217.1"
                     /translation="MAKPKARLLNIWLVLLLAPTLYACGSDTPPTPTIVPTAAPTSAV
                     LPTSGVPDAPPPVLEPTSDPATGDAGTLMGLPVGVFENAQTKAVQQGQPRQVQSAQAR
                     IRSTDLPNTRELGAPSWVRPGMRITFYAAAATVVSRGEPKCKEDPNGEWEDQAGNKFS
                     CTGMPGDPDGSSAGDGLSQVDVLAVEGTDVVLATTLFSIDRISNQFTVAPTGGGKAPG
                     DVIDGVWIHPRRLAEIAAMDLQDMKVLRGNYEVEGVTYNAISFVSLKPGSYQSYAYDT
                     ETGLQIAATTRTTPSTTLLEAPQTAGNGSAQLYVSRLLGYRQRNVPGLNGTNPAWVAR
                     TRQLNYNGTWTFTNPMGSGSPSDTMAFPMDLSVALSRGGKNWVPFTARSAIHFPPDQV
                     STGSGVTGSAGQYWIDPKALARLKRGQVIDEDPRTNERLVVESVGTRAGAKTVVISSE
                     LPGNRSLLTYDLTTGALVDFQTSSSGTGITILLRLQTMPR"
BASE COUNT          449 a          753 c          708 g          376 t
ORIGIN      
        1 gctgcgccct gtctccagca tgcccaacgc agagacaatg accgggacga ccagttcagt
       61 gccgtctgcc gctggcattg atacctggag gatgccgtcg ccgaagtctg cctgcacatt
      121 gtgagcaact taccgggcga gccttatctt gccgacaccc ggcgtactcg tcacagtcag
      181 caaaccaacg tcagggccag ccaggaggcc cctgcaagcc tccaagtact ctcttgcggt
      241 ttacttgcct attgaggagt gcaggcaggc taggagggcc gctgaagggc tgtcagggcc
      301 tgtcttgagt gctgtcagca tatcgcgccg gaggcggtgc cgcgccaggc ctatccggct
      361 ttcccgcgcg aactgtatga aggccgcctg ctctcccgcc ggtacaggct gtccccagag
      421 tgctacctcc ccagcttacc ccagaacgta gctaaccgct gtggcagagc cgcccaggct
      481 gcgctataat tggagcgctc cacatgacga cccgccctct cttgagcgct gtcttaccgt
      541 ctacaatagg aggaaagcct tggctaagcc gaaagctagg ctgctgaaca tctggctggt
      601 gctgcttcta gcaccgaccc tgtacgcgtg cgggagtgac accccaccta cgcccaccat
      661 cgtgcctacc gccgcgccaa cctccgccgt cctgcctacg agtggagttc cggatgcgcc
      721 cccgccggtt ttggagccaa cctccgaccc tgctacgggc gacgcaggca ccttgatggg
      781 gcttcccgtg ggtgtattcg agaacgccca gacgaaagcc gtgcaacagg gccagccccg
      841 gcaggtgcag tcagcacaag cccgaatcag gagcaccgac ctacccaaca cgcgagagct
      901 aggcgcgccg tcgtgggtga ggccgggcat gcgcatcacc ttctatgcgg cggcggccac
      961 ggtggtctcg cggggcgaac ccaagtgcaa ggaagacccg aacggcgagt gggaggacca
     1021 ggcgggcaac aagttcagtt gtacagggat gcccggcgat cccgatggca gctccgccgg
     1081 tgacggcctg agccaggtag acgtgctggc ggtggagggc accgacgtgg tgcttgctac
     1141 caccctcttt agcatcgacc gcatcagcaa ccagtttacg gtggccccaa cgggcggcgg
     1201 caaggcccca ggcgacgtaa tcgacggggt atggatccac cctaggcggc tggccgagat
     1261 agccgctatg gacctccagg acatgaaggt actgcgaggc aactacgagg tggaaggcgt
     1321 gacctacaac gccatcagct tcgtcagcct caagcccggc tcctaccagt cgtatgctta
     1381 cgacaccgag acggggctgc aaatcgcggc caccacccgc accacaccct ccactacgct
     1441 gctcgaagcc ccgcagacgg cgggcaacgg cagcgcgcag ctttacgtga gtcgcctgtt
     1501 aggctaccgg cagcgcaacg tgcccgggct caacggcacc aaccccgcct gggtggcgcg
     1561 gacccggcaa ctgaattaca acggtacctg gaccttcacc aacccgatgg gtagcggcag
     1621 ccccagcgac actatggcct tcccgatgga cctgtcggtg gcgctctcca ggggcgggaa
     1681 gaactgggtg ccgttcacag ccaggtcggc catccacttc ccaccggacc aggtctccac
     1741 cggcagcggc gtcacgggca gcgcgggcca gtactggata gaccccaagg cgctcgccag
     1801 gctcaagaga ggccaggtga ttgacgagga cccgcgcacg aacgagcggc tcgtcgtgga
     1861 gtccgtcggc acgcgcgcgg gggccaaaac ggtggtcatc agcagcgagc tacctggcaa
     1921 caggtcgctg ctcacctacg acctcaccac gggcgcgctg gtggactttc agaccagctc
     1981 cagcggcaca ggaataacga tcctcctccg gcttcaaacc atgccgcgct agggaggcag
     2041 acgccctgcg gcgttgccct gcggcgttgc cctgggggtg taccctaggg ggtgccctga
     2101 gggggtgccc tgccaatgaa ttggcgggct taccagaggc aaagtcccct tcggggactg
     2161 aggaacgtga gggtagggtc tatctgcaaa gtatatgaag caatagaaca cgggtggtcc
     2221 ttgtcattct gagcgtagca aagaatctcg tcctccatgc aagtgttgcc cttttccata
     2281 gcacct
//
LOCUS       DASRWZ010000650         6221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1354208, whole genome shotgun sequence.
ACCESSION   DASRWZ010000650 DASRWZ010000000
VERSION     DASRWZ010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354208"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VFH60_10300"
     CDS             <1..1212
                     /locus_tag="VFH60_10300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6314218.1"
                     /translation="LAFTVQAIYFILNADLDPLQLVLVGTVLEVSCFLFEIPTGIVAD
                     VYSRRLSVLIGFALWGLGFLIEGAFPFFWTILVSQVVMALGYSFNSGALEAWIAGEVG
                     EENLGRTFLRASQVGRISSLAALVAIIVLGLGNINPAALILVAGVGFLGLAVYGKFAM
                     PETAFTRKPKEEREGWRDMATTFKDGTRVVRASPVLLIFMALTLFAGASTEGMDRLWE
                     AHFLQNFTFPGGAGLQPIVWFGLMNIGLLILGVLGTYILQKRVDTNNRNVVARVLMAF
                     TVLEIASLLWFALSGDFAQALLAFWGMNLFRGSAGPLFRTWLTRSIEPRVRATVLSMV
                     GQSDAIGQMTVGPVIGWIGTAGSIRAALMFSAVLLAPVVPLVAMARNRVVAKPSEPAT
                     ILPEVPVALGD"
     gene            complement(1382..2740)
                     /locus_tag="VFH60_10305"
     CDS             complement(1382..2740)
                     /locus_tag="VFH60_10305"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET6314219.1"
                     /translation="MESSTLQRTPAIGMDTPAPQAPSGMRLLRMVAIATCAALLLVYL
                     LGWYAAMRDPQMHGFDGVVRRSDFLSNLTGAKLIAEGRGPDLYNLDAQREAQNAVLAP
                     YFKLDPGKILPYNHIPFEAMLTAPFVQAGVPYTAIFTLWELLMLGLLAASIWSMQRVL
                     PVHGAALFVLVLAAVSYQPVARSFILGQNSPMVLLGICLVYAASRHKQDVWVGLALLL
                     VAPKPQVLPVIGLVLLLQGRWRGLLVFGGAFGALCVAAMPVLGVDWPWQYARLLVGVA
                     GWQDTGAIDPGIMHNWRGFVTNLFGAWAPGLVTPLFLLLTLLSVGLVVWLWLRTRGTG
                     EAESAPYLARFDKLWAVAGILAVLTSLHLNPHDLVLLIFPAWIVGAYALSGAFGGASR
                     LWLGLLWLGYALIPAAFFLQNVPGVKVGGVVLSVLLMAGAALWLATQRGDGTDRAAIK
                     PT"
     gene            complement(2754..3980)
                     /locus_tag="VFH60_10310"
     CDS             complement(2754..3980)
                     /locus_tag="VFH60_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314220.1"
                     /translation="MWNRVFAVLVTVVVTTLIAGPIVSGQDLITPDSQRGLRNDHYYY
                     LARNMVQGNLTVDGIPDFYPDWVEWNGHRYLPLGPLPAVLLIPFLPVLELGLDPVAIG
                     LLFTLLNAWLLYKVLMSIGIRDARLRWSLLLFFGGTVYLSTAMKSGAWFLAHIVTITF
                     LLMAVWAVVGRKLPLAAGVLLGLAGMSRTTALFALPFFVAMLLYKGGIEGDRVTRGEL
                     AKRVGMLLLGVSGPVLLLGLYNYARFGSPLDTGYSHVVLQTSVLSDALRHGLFSLEHI
                     PKNLFMLLVQGPLPYPNENAAVLSFPYLRPSPWGMGIFFTSPALLYIFRANRRKPFVL
                     ACWLAVACVMIPIITYYGVGWIQFGYRYALDFTPFLVLIAALGFPAVMSNKVRVLVML
                     SVLVNVWGSIFLMKSY"
     gene            complement(4347..>6221)
                     /locus_tag="VFH60_10315"
     CDS             complement(4347..>6221)
                     /locus_tag="VFH60_10315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6314221.1"
                     /translation="RATRAAWTPALLALGGLVLVAAGLRFYCLDCYGLWYDEVSSVQV
                     AQRGVVAILTDRFGWMRVQTPLHYLLVRLSIQPLDPTTSEVLVRLPSALAGLLTPVVV
                     YGLGRETFGRAQGLLAALFVAASAVHVSHSQDARPYVFLALFTALSVYCLLLCERTGE
                     RRWWFAFAASTGANLLMTYHALTLAMPAIAPYLLWVLYKVWRGRGAEEGKAGLRGVAV
                     SLAAIGVVGVVMLADILRVPRIPPDLSLFSPASVGDQLGRMLNRLGQVGVDREAEKVL
                     QWLVAAVALLGVLLGVRERRFRGVLLCLLMLLVPALLMAVLRTTNSVFQRYVLFAMPV
                     FLLLVANGSVGAGVLVLARWKWKQGGRAVSGALGAGALMLFGLGLYVYFSPEQHKQLS
                     FLPDYRGAARYLTERVKPRDLIVLLEEPPQGMQVLDFYWHGSPPAPTFVGIDPRLHAQ
                     PAPRNIYWVMTYALNDPEYLAALQAASPPGRDFASVAEFDRLLVLEENNPGGVLPSLR
                     RMADRMAVANPPFSPSLQALYTLQGSLLQAGGDTQGAANMYRTAGPFYLLGDEYLTTS
                     QGFHSRGDRSAAWREAIMSLFLEPYRPQIHAYLAQLLNEEALIPQGTLEQQVATDLER
                     "
BASE COUNT         1235 a         2004 c         1957 g         1025 t
ORIGIN      
        1 ctggccttca cggtgcaggc catttacttc atactcaacg ccgacctgga cccgctgcaa
       61 ctggtgctgg tgggtaccgt gctggaagtg tcgtgcttcc tgtttgagat acccaccggg
      121 atcgtggccg acgtctacag ccgccgcttg tctgtgctga taggtttcgc gctgtggggt
      181 ctgggcttcc tcatcgaggg tgccttcccc ttcttctgga ccatcctggt gtcgcaggtg
      241 gtcatggcgc tcggctactc gttcaacagc ggggcgctcg aagcgtggat agccggggag
      301 gtgggcgagg agaacctggg ccgcaccttc ctgagggctt cgcaggtggg ccggataagc
      361 tccctggcgg ccctggttgc gatcatcgtg ctcggcctgg gcaacatcaa ccccgccgca
      421 ctgatcctgg tggccggtgt cggcttcctg gggctggccg tctacggcaa gttcgccatg
      481 cccgaaaccg ctttcacacg caagccgaaa gaagagcgag aaggctggcg cgacatggcg
      541 accaccttca aagacggcac cagggtggtg agggccagcc cggtgctcct catcttcatg
      601 gcgctgaccc tcttcgcggg cgcatctacc gagggcatgg accggctgtg ggaggctcac
      661 ttcctccaga actttacctt tccgggtggg gccggcctcc agccaatcgt ctggttcggc
      721 ctgatgaaca taggtctgct catcctgggc gtgctgggca cctatatcct ccagaagcgc
      781 gtcgatacca acaatcgcaa cgtagtggct cgcgtgctga tggcttttac ggtgctggag
      841 atagcatctc ttctctggtt cgcactctcg ggagacttcg cacaggccct gctggccttc
      901 tggggcatga acctcttccg aggcagcgcc ggccccctgt tccgaacctg gctgacccgg
      961 agcatcgagc cgagggttag ggccaccgtg ctgtcgatgg tcgggcagag cgacgcaata
     1021 ggccagatga cggtggggcc ggtaatcggc tggataggca cggcaggctc catacgcgcc
     1081 gcgctgatgt tctccgccgt gctgctggct cccgtagtgc ccctggtggc gatggcccgc
     1141 aacagggtgg tagccaagcc ctctgagccg gcgaccatcc tgcccgaagt gcccgttgcg
     1201 ctgggtgatt agcagagacg cagagagcgc agaggttacg caaagattag gattgtttgg
     1261 gaatggacag gggcagcatc gtactgtgcg atgccgcccc tgtaagaaca ccctccatcc
     1321 ttctctgcgt aacctctgcg ccctctgcgt ctctgcggtg aattctttac ttgggcaggc
     1381 gttatgttgg ctttatagcc gccctgtcgg tcccgtcccc acgctgcgtg gcaagccata
     1441 gtgccgcccc cgccatgagc agcacgctca gcaccacgcc ccccaccttc actcccggca
     1501 cattttgtaa gaagaaggca gccgggatga gagcataacc cagccatagc agccccaacc
     1561 ataaccgcga cgcgccaccg aacgcgccgg atagggcgta cgcgcccact atccaggccg
     1621 ggaagatgag cagcaccagg tcgtgcgggt tgaggtgcag ggaggtgagc accgccagga
     1681 tgcccgccac cgcccacagc ttatcgaacc gggcgagata tggggcgctc tctgcttccc
     1741 ctgtgcctct tgtccttagc cacagccaca cgaccaggcc caccgacagc aacgtcagca
     1801 gcaagaagag cggagtcacc aggccgggtg cccacgcgcc gaacaggttg gtgacgaagc
     1861 cgcgccagtt gtgcatgata ccggggtcga tagccccggt gtcctgccag cccgccacgc
     1921 ccaccagcag ccgcgcgtac tgccacggcc aatcgacgcc caacactggc atggccgcca
     1981 cgcacagcgc cccgaaggcc ccgccaaaca cgagcagccc cctccaccgc ccttgcagta
     2041 acagcaccag ccctatcacg ggcagcacct gcggcttggg ggccaccagc aacagcgcca
     2101 gccccaccca cacgtcctgc ttatgccggg aggccgcgta caccaggcag atgcccagca
     2161 gcaccatcgg ggagttctgc cccaggataa aggagcgcgc taccggctgg tacgatacgg
     2221 ccgccaggac caggacgaac agcgcggccc cgtgcaccgg cagcacccgc tgcatgctcc
     2281 atatcgaggc cgccagcagc cccagcatca gcagctccca cagcgtgaat atagcggtgt
     2341 agggcacgcc cgcctgcacg aacggcgcgg tgagcatcgc ctcgaagggg atgtggttgt
     2401 agggcagtat cttgccgggg tcgagcttga aatagggggc gagcacggcg ttctgggcct
     2461 cgcgctgggc gtccaggttg tagaggtcag gcccccggcc ctcggcaatc agcttcgcgc
     2521 ccgtgaggtt ggagaggaag tcggagcggc gcaccacgcc gtcgaagccg tgcatctgcg
     2581 ggtcgcgcat cgcggcgtac cagccgagca ggtagaccag cagcaacgcc gcgcaagtgg
     2641 caatcgccac catcctcagc aggcgcatac cggacggggc ctgtggggct ggcgtgtcca
     2701 tacctatcgc gggcgtcctt tgcaaggtcg aagactccat atcgcgtgct atatcagtaa
     2761 gacttcatca ggaatatact gccccacacg ttcacgagca cggacaacat gaccagcact
     2821 cgtactttgt tcgacatcac ggcagggaag cctagcgccg caatcagcac caggaagggc
     2881 gtgaagtcga gcgcgtagcg gtagccgaat tgtatccagc cgactccata ataggtgatg
     2941 atcggtatca tcacgcacgc gaccgccaac cagcatgcta acacaaaagg cttgcggcgg
     3001 ttggcgcgga agatatagag cagcgcggga gaggtgaaaa agatgcccat gccccagggc
     3061 gaggggcgta ggtaagggaa gctgaggacc gccgcgtttt cgttgggata aggaaggggg
     3121 ccttgcacga gcagcatgaa caggtttttc ggtatgtgtt ccaggctgaa cagcccgtgg
     3181 cgcaacgcgt cggacagtac cgaggtctgc aacacaacgt gagagtagcc ggtgtcgagg
     3241 ggagagccga agcgggcgta gttataaagc cccagcagca gcaccgggcc tgacacaccc
     3301 agcaacaaca tgcccactct tttcgccagt tcccctctcg ttaccctgtc tccctcgatg
     3361 ccgcccttgt acagcagcat cgccacgaag aaaggtagcg cgaacaaggc cgtggtccgc
     3421 gacatgcctg ccagccccag cagcaccccg gcggcaagag gcagcttcct acccactacc
     3481 gcccacaccg ccatgagcag gaaggtaatc gtaactatgt gggccaggaa ccaggcccca
     3541 ctcttcatcg cggtgctgag atagaccgtg cccccgaaga agagcaggag cgaccacctc
     3601 agccgggcat ccctgattcc gatgctcata agcaccttgt acagcagcca tgcattgagc
     3661 agcgtaaaga gcagacctat agccaccggg tcgaggccaa gctccaacac cggaagaaaa
     3721 gggatgagca gcacagcggg gagaggccca aggggtaagt agcggtgccc gttccactcc
     3781 acccagtcgg gatagaaatc gggtatgcca tcgacggtga ggttgccctg caccatgttg
     3841 cgggcgaggt agtaatagtg gtcgttgcgc agcccgcgct gggagtcagg cgttatgagg
     3901 tcctgccccg atacgatagg ccccgctatc agcgtggtta ccactaccgt caccaggacc
     3961 gcgaagaccc tgttccacaa gggaccctct ccctgtggcg tagcttttac ctcgttccca
     4021 tatgcaggaa tagactgtac gctattcaac aagatcacct ttcgtgcgtc gattctgagg
     4081 gatgttcgcc taccttagcg catgggggtt ttgcatgaag ctacgacagg tatggtactg
     4141 cacagcgccc tgtgacaggt gggagctcgg accgggctgt aattcacgtc ccagccggga
     4201 caatagtcct aatccatcct gaataagtct accatcgggc tactcccagc gaaatagccc
     4261 cacagggcca tccggcatgc gacataggta ctgtagattt tagattttag attcagtaga
     4321 acttcgagct aatccaaaat ctaaaatcat ctttccagat ccgtggctac ctgttgttcc
     4381 agcgtgcctt ggggaatgag ggcttcttcg ttgaggagtt gggcgaggta ggcgtggatt
     4441 tgggggcggt atggctccag gaaaagggac atgatggctt ctcgccaggc ggcggagcgg
     4501 tcgcccctgg agtggaagcc ctgggatgtg gtgaggtact cgtcgcccag caggtagaaa
     4561 ggcccggcgg tgcggtacat gttggcggcc ccttgcgtgt cgcccccggc ttgcaggagg
     4621 ctaccttgca gggtgtagag ggcttgcagg ctgggtgaga agggcgggtt ggctacggcc
     4681 atcctgtccg ccatgcgtcg caggctgggc agcacgccgc cggggttgtt ctcctccagc
     4741 accagcagcc ggtcgaactc ggccaccgag gcgaagtcgc ggccgggggg cgaggcagct
     4801 tgcagggccg ccaggtactc ggggtcgttc agcgcgtagg tcatcaccca gtagatgttc
     4861 cggggggcag gctgtgcgtg caggcgcggg tctatgccga cgaacgtagg ggcgggcggg
     4921 ctgccatgcc agtagaagtc gagcacctgc atgccctggg gcggctcttc cagcagcacg
     4981 atcaaatcgc gcggctttac acgctcggta aggtagcggg ccgctccccg gtagtcgggc
     5041 aggaaagaga gttgcttgtg ctgctcaggg ctgaaataga cgtacaggcc aagcccgaac
     5101 agcatcagcg cccccgcgcc caaagccccc gacaccgcgc gtcctccctg cttccacttc
     5161 catctggcga gcacgagcac gcccgcgccc acactgccgt tggcgacgag cagcaagaag
     5221 actggcatgg cgaagagcac gtaccgctgg aacaccgagt tggtggtcct tagcaccgcc
     5281 atgagcagcg cgggcacaag cagcatcagc aggcacagca acacccctcg aaagcgccgc
     5341 tcccgcacgc ccaacagcac gcccaacagg gccacagccg cgacaagcca ttgcagcacc
     5401 ttctcggcct cgcgatctac ccccacctgc cccagccggt tgagcatcct gccaagctgg
     5461 tcgcccaccg aggcggggga gaacagcgac aggtcgggcg gaatcctggg cacgcgcagt
     5521 atgtccgcca gcatcaccac cccgaccaca ccgatagccg ccagactgac cgccacgccg
     5581 cgaagccccg ccttaccctc ctccgcaccc cgcccgcgcc acaccttgta gagcacccac
     5641 agcaggtacg gggctatggc gggcatggcg agggtaagcg cgtggtaggt catgagcagg
     5701 ttggcgcccg tgctggcggc gaaggcgaac caccaacgcc gctccccggt gcgctcgcac
     5761 agcagcaggc agtagaccga cagggcggtg aagagcgcca ggaagacgta gggccgcgcg
     5821 tcctgcgagt ggctgacgtg cacagccgag gccgccacga agagggccgc gagcaggccc
     5881 tgcgccctcc cgaacgtctc gcgccccagc ccgtagacca ccacaggcgt gagcagcccc
     5941 gccagcgccg acggcaggcg caccagcacc tcgctcgtcg tggggtcaag cggctggatg
     6001 ctgagcctca caagcaggta gtgaagcggg gtctgcacgc gcatccagcc gaagcggtcg
     6061 gtcagtatgg cgaccactcc ccgttgcgcc acctgcaccg aagagacctc gtcgtaccag
     6121 aggccgtagc agtcgaggca gtagaagcgc agcccagccg ctacgagcac gaggcccccg
     6181 agcgcgagca gggcaggcgt ccaggccgcg cgggtagccc t
//
LOCUS       DASRWZ010000651         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_738310, whole genome shotgun sequence.
ACCESSION   DASRWZ010000651 DASRWZ010000000
VERSION     DASRWZ010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738310"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFH60_10320"
     CDS             complement(<1..421)
                     /locus_tag="VFH60_10320"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET6314222.1"
                     /translation="MRKQILDEIIESKCLLKHPFYQAWNMGTLTKGDLAVYATQYGEF
                     IDTIATGWEAVGDADTAAEEREHAVLWTDFASGLNAPRPAESRLPEITELVTLAQATF
                     SNRPQALGALYAFEHQQPATAHSKLEGLRRHYNLPTSA"
     gene            complement(765..1940)
                     /locus_tag="VFH60_10325"
     CDS             complement(765..1940)
                     /locus_tag="VFH60_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314223.1"
                     /translation="MSQYYPIPQPTIATNILVRRERRLPVPGEVVVRAGQRVEPSDVL
                     AQSTLGAEPVQVGIAADLDMSPAAAGKRLRVSNGQTVEKGAVLAQKGGLGSRVSRSPI
                     TGTFTGYDSNSGIGLISLPSEIVSVQAHLKGIVTELIPYYGAVIETPATLIRGIFGIG
                     GEQHGVLRVLVSGNDEPVTPELIDARVSYSVALGGSEVTVAALHKMIELGARGLITGS
                     IRASELGEFLGYRGHGSWRLGATSMSGNGWDFPPPSSAVPSPVPPDFVLIITEGFGPV
                     PMSARTFEMLAAYDGQEIAIDGTTRLRGGLSRPEIVIPLARTTAVRWLEETGPKVEVG
                     KHVRLLAQAYLGQVATVISLPVGPRASQSGVLTTVAEVQLGTGQRLRVPIVNLEVLE"
     gene            complement(1984..2220)
                     /locus_tag="VFH60_10330"
     CDS             complement(1984..2220)
                     /locus_tag="VFH60_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314224.1"
                     /translation="MLQTSVRDKIINRLDGFTVEQQQKILEYMESLASRLPAGVGTAG
                     SNLLHYAGTLPKEEAEEMLRAIEEECECIEPYQG"
     gene            complement(2289..>3877)
                     /locus_tag="VFH60_10335"
     CDS             complement(2289..>3877)
                     /locus_tag="VFH60_10335"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HET6314225.1"
                     /translation="LDEGLHQLPNEDEAVLNSAAIAWSQEQTDKLLALPPDVVLMSGG
                     VDGGPVSPLVRLAKVVAVTAREQGSRAELAARKGQAAPGMPMLIFAGNREALQGVTDA
                     LVPISEMLSVPNVRPDLHTETPGPAAAAIAGIYTERRVPQIPGYHVLSRWVEGPIVPT
                     ADCERLIAQYLHRQYARETLVVDVGATSTGLFLANGLGDSSAVMGDMGMAYGLTNLLA
                     TRGAASVLRWLPFEMSEEELWEWALNKVARPLTLPQTPRDLMIEHALAREALLHGAEA
                     LQGYNGGRPPRYDLLVGTGGLLAHTPRPGQAALLLLDALQPAAVELGSVELALDTTLL
                     IPPLGNLARHQGAAASYIFDRDCLVWLGTAIVVHNAWSDTGNANGTGKAPAAVPGSTI
                     AVNVTVERQQGGTESVEVPYGTIRIVPLRPGQRAALTVKPGAGFRVGSGEPGKALKTQ
                     PGQEVKGGLVGLVIDARGRPLDLPEDGTVRRTMIRGWLAAMDAIAESGTGQAPGARGL
                     EIVPPPDAQGEAEPEGEGGV"
BASE COUNT          619 a         1361 c         1183 g          714 t
ORIGIN      
        1 ctgccgacgt aggaaggtta tagtgcctgc gcaggccctc aagcttgctg tgggcggtag
       61 cgggctgctg gtgctcgaag gcgtatagag cgcccagggc ttgcgggcgg ttgctgaacg
      121 tggcctgggc tagcgtcacc aactcggtta tctccggcaa gcgggactcg gcgggacgtg
      181 gggcgttcag gccggaagca aaatcggtcc acaacaccgc gtgctcccgc tcctcggcag
      241 ccgtatcagc atcacccacg gcttcccagc ccgtggctat agtatctatg aactcaccat
      301 actgggtcgc gtaaaccgcc aggtcgccct tcgtcagcgt gcccatgttc caagcctggt
      361 agaaggggtg cttcaacagg cacttgctct caattatctc gtccaggatt tgctttctca
      421 ctttattctc ctattgtcac tttagtagct cgactggcct gccgggaaca catggtacga
      481 cttagcaggt tcacgctgca aacgtgcagc cctgggcatg attgtagctc atagctaccc
      541 tgtttgcaac tgccccagga ggcagcgcgc cctgcacacc caagcaataa cactgtagcg
      601 cattctttac gtactttgcg cccttgtggt ttcgtttctt actaagagcg tcttctaaag
      661 ggtcaggaca ttactcgtgg gggcaggccc ccgtgcctgc tcctacgcat ctttgggtta
      721 gaatggcgct cttagtggct tcgcctgacc ctgtgaccct gtgcctattc cagcacctca
      781 aggttcacga tgggcacccg cagtcgctgc ccggtgccca attgcacctc ggccaccgtg
      841 gtgagcacgc cggactgcga ggcgcgcggg cctaccggca agctgatgac tgtggcgacc
      901 tgcccgaggt aggcctgggc cagcaggcgc acgtgcttgc cgacctccac tttaggcccg
      961 gtctcctcca gccagcgcac ggcggtggtg cgggccagcg gtatcactat ctccgggcgc
     1021 gacaggcccc cgcgcagccg ggtcgtgccg tcaatcgcta tctcctggcc gtcgtaggcc
     1081 gccagcatct cgaacgtgcg ggcggacatc ggcacggggc cgaaaccctc ggtgatgatg
     1141 agcacgaagt cgggcggcac gggcgagggc acggcggagg agggcggggg gaagtcccag
     1201 ccgttgccgc tcatgctggt agctcccagc cgccaggagc catgcccgcg atagcccagg
     1261 aactcgccaa gctccgaggc ccgaatgctg cccgtgatga ggccgcgcgc cccaagctcg
     1321 atcatcttgt gcagcgccgc gaccgtaacc tccgagccgc caagcgccac cgagtaggac
     1381 accctcgcgt cgataagctc cggcgtcacc ggctcgtcgt tgcccgacac gagcaccctg
     1441 agcaccccgt gctgctcgcc ccctatgccg aagatgcccc ggatgagggt ggcgggggtc
     1501 tctatgaccg caccatagta gggtatcaac tcggtcacga tgcccttgag gtgggcctgc
     1561 acagacacga tctcggaagg caaggaaatc aggccgatgc cgctattaga atcgtaccct
     1621 gtgaaagtgc ccgtgatggg cgacctcgac acccgcgagc caagcccgcc cttctgcgcc
     1681 agcacggccc ctttctccac agtctgcccg ttgctcacgc gcaacctctt gccggcggcc
     1741 gcgggcgaca tgtccaggtc ggcggctatg cccacctgca ccggctccgc gcccagcgtg
     1801 ctctgcgcca gcacatcgga aggctcgacc ctctgccccg cccgcaccac cacctcccca
     1861 ggcaccggca agcgccgctc ccgcctgacg aggatgttgg tggctatcgt gggttggggt
     1921 atagggtagt actggctcac ctgtgtctcc ctctgtctct gttcgtctgc cgctgtctgt
     1981 gcctcagccc tggtatggct cgatacattc gcattcttct tctatggcac gcagcatctc
     2041 ttccgcttcc tccttgggga gggtacccgc gtaatgaagt aagttgctgc ccgcagtccc
     2101 aacgccggca ggcagtcgtg aagctagaga ctccatatat tccagtatct tttgttgctg
     2161 ctcgaccgta aaaccgtcca gcctgttgat aatcttatct cttaccgacg tttgaagcat
     2221 tccagcacct cctaccgtca cccactacga atgcttctac cattataagc cttcgcgcca
     2281 caccaagact atacgccgcc ctcaccctcc ggctccgctt cgccctgggc gtcgggaggc
     2341 ggcacgatct ccagccccct cgctccgggg gcctgcccgg tgccgctctc ggctatcgcg
     2401 tccatcgcgg cgagccaacc tcgtatcatg gtgcggcgga ccgtgccgtc ttccggcagg
     2461 tcgagcgggc ggccgcgggc gtcaattacc agccccacca gcccgccctt gacctcctgg
     2521 cccggctgcg tcttgagggc cttccccggc tcgccgctgc ccacccggaa gcccgccccc
     2581 ggcttcacgg ttagagccgc gcgctgtccc ggccgcaggg gcacgatgcg tatagtgccg
     2641 tagggcacct ccacgctctc ggtgccgccc tgttgccgct ccaccgtgac gttcaccgcg
     2701 atggtgctgc cgggcacggc ggcgggcgct ttaccggtgc cgttagcgtt gccggtgtcg
     2761 ctccacgcgt tgtgcaccac aatcgccgta cccagccaca ccaggcagtc gcggtcgaag
     2821 atgtaggagg cggcggcccc ctggtgccgg gccagattgc ccagcggcgg gatgagcagc
     2881 gttgtgtcca gcgccagctc taccgaccca agctccacgg cggcgggctg caaagcgtcg
     2941 agcagcagga gggccgcttg tcctggtcgg ggcgtgtggg cgagcaggcc gccggtgccc
     3001 accagcaggt cataccgggg cgggcgtccg ccgttgtaac cctgcaacgc ctccgccccg
     3061 tgcagcaagg cttcgcgggc caaagcatgc tcgatcatca ggtcgcgggg tgtctggggt
     3121 agcgtgaggg ggcgggccac cttgttgagc gcccattccc aaagctcctc ctcgctcatc
     3181 tcgaacggta gccagcgcag cacgctcgcc gcgcccctcg tggcgaggag attcgtcagg
     3241 ccgtaagcca tgcccatatc gcccatcacc gccgagctat cgcccagccc gttcgccagg
     3301 aacaacccgg tggaagtggc ccccacgtcc accaccagcg tctcccgcgc gtactgcctg
     3361 tggaggtact gcgcgataag acgttcgcaa tcggcggtgg gcacgattgg cccctccacc
     3421 cagcgcgaga gcacgtggta gccgggtatc tggggcacgc gccgctcggt atagatgccg
     3481 gctatggcgg cggcggcggg gccgggcgtc tcggtgtgca ggtcgggccg cacgttgggc
     3541 acgctcaaca tctcggaaat cggcaccagc gcgtcggtaa cgccctgcaa cgcctcccgg
     3601 ttgcccgcga atatcagcat cggcatgccg ggggcggcct ggcccttgcg cgcggctagc
     3661 tcggcccggc tgccctgctc ccgcgccgtc actgccacca cttttgccag cctcacgagg
     3721 ggagacaccg gcccgccgtc caccccaccc gacatgagca ccacgtcggg cggcagggcc
     3781 agcagcttat cggtctgctc ctgcgaccat gcgatagccg cgctgttgag caccgcctcg
     3841 tcctcattgg gcaactggtg caggccctcg tccaggg
//
LOCUS       DASRWZ010000652         4385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_121645, whole genome shotgun sequence.
ACCESSION   DASRWZ010000652 DASRWZ010000000
VERSION     DASRWZ010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121645"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..950)
                     /locus_tag="VFH60_10340"
     CDS             complement(96..950)
                     /locus_tag="VFH60_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314226.1"
                     /translation="MNANSSNSHNGNHNGNHISSQALSVLSLFADAHDEGDLSKASFQ
                     ALSVPDLGAQIQAAMGVPALDVPATRAILLTLLLDDSGSIAAGGNEQAVRDGYNEVVK
                     ALRESRQADDILAQCSYLNRGILYPYEFIGHVPLLDVRNFSAYGITPLYDRAIVTLGA
                     VLAKSREFTASGVPTNTVTLIVTDGHDEGSVHRARDVYPIVQDMLAQESHIVAGMGID
                     DGDTDFRAVFRSMGVPDNWILTPRSDPKEIRQAFQLFSRSAISASQSQATFSQTAASG
                     FTVGGFGV"
     gene            complement(1020..2132)
                     /locus_tag="VFH60_10345"
     CDS             complement(1020..2132)
                     /locus_tag="VFH60_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase"
                     /protein_id="HET6314227.1"
                     /translation="MATQITTQNGNTGGLLPTLPIPPHFDPSRADKVWPVDYLRIERE
                     AEEWAARHDIEPSASDRVRTALLVIDAQITFCIPGYELYVGGRSGRGAVEDNIRLCEF
                     IYRNMPHITRIFPTMDTHTQYQVFHPLFWVNDSGRHPDPYTFISHDDIVRGVWKVNPR
                     ATYSLGGNYLALQRHVEHYTAELEKSGKYALCVWPPHAMLGGIGHALAPIVHEAVEFH
                     NAARAAQTGFEIKGGHPLTENYSVLAPEVLTTTGGAPLPNAQRNYKFLEKLIDPMSGF
                     EVIGITGQALSHCVAWTIDDLLRDILQKDPAAARKVHIIRDCSSPVVTPGYDFTPDAE
                     AALQRFERAGMHIVTSDQPVTEWPGYREALAAPVTR"
     gene            complement(2173..4170)
                     /locus_tag="VFH60_10350"
     CDS             complement(2173..4170)
                     /locus_tag="VFH60_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314228.1"
                     /translation="MGTRKVYVGGRTLTLQAAQAIGTGGEADVYSIGGGLALKVYKSP
                     DHPDLQSANPVERAALKAAAQERLDTAQRKLRVFPRNLPSSVLGPLELASDGPGTGAC
                     VVGYTMPYLAGADLLYQYGKRKFREDALQTGLTPERVRDVLLDLHGTVRAVHSAGVVI
                     GDFNDLNVLVRDDITYLVDADSFQYGGFLCRTFQYRFIDPLICDPRLDHLELSRPHTQ
                     ESDWYAYSVIALQCLLFVDPYAGLYKPKAAGKSKGKISESTRPLHRITVFSPDVQYPR
                     PAIPYRALPDELLEYFRETFEGDRRGEFPVRLLQDLRWSRCPLCGADHAHPTCPWCQP
                     QTCVQVPPVVPLQTVRGDVTATLVFRTEGRVVAAAVRDGQLCWLYQQDGELRREDGST
                     LLRWGLVKGIRVRLSGNATHVARGANLVSLRAGAPPERRVIETVTGAGASAIPAFAAN
                     SAHLYWVEGGRLYRDSALGPYAIGDVLSHQTRIWAGERFGLGHYRAGGLEGALLFDAE
                     HAGVNDRVDLALPPGQLLDATCSFGHDRCWFLAAIKGPKGVTNHCVLVARDGHVLGRH
                     AAPEGDGSWLGTLRGKCAAGNSLLCATDDGVARVDELSGALVQTRLFPDTAPYVDSAT
                     QLFAGRDGLYAVSDRTIYLLRLGNAGNTVPPPGGVLATHPI"
     gene            complement(4157..>4385)
                     /locus_tag="VFH60_10355"
     CDS             complement(4157..>4385)
                     /locus_tag="VFH60_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314229.1"
                     /translation="GKEDVAGGLDPWWREDLYYRNAAALPNRLRLLGTDCQRIDWDAR
                     RKLTDWGRVEDDATLVVGRRAPREEVAHGNA"
BASE COUNT          721 a         1400 c         1478 g          786 t
ORIGIN      
        1 actaatggca acaggggaca aagacgatac gagaggcacc cggagcccgt agatctccgg
       61 gtgcctctcg cagtggacct acagtcgctt cggcgtcaca ccccgaagcc gcctaccgta
      121 aagccggacg cggcggtctg cgagaaggtg gcctggcttt gagaggcgga tatcgctgag
      181 cgcgagaaga gctggaacgc ttgccttatc tcctttgggt ccgagcgcgg agtcaggatc
      241 cagttgtctg ggacgcccat tgagcgaaat acggcccgga agtcagtgtc tccgtcgtcg
      301 atgcccatgc ccgcaactat gtgcgactcc tgggcgagca tgtcctggac gatgggatag
      361 acgtcgcgcg cccggtgcac ggaaccctca tcgtgtccgt cggtgactat gagcgtgaca
      421 gtgttggtgg gcaccccgct cgctgtgaac tcgcgggact tggcgagcac cgcccccagc
      481 gttactatgg cgcggtcgta aagcggcgta atgccatatg ccgagaagtt acggacgtcg
      541 agcaggggca cgtgcccgat gaactcgtat gggtagagta tgcctcggtt gaggtagctg
      601 cactgcgcaa ggatgtcgtc cgcctgccgg gattcgcgca gggccttcac cacctcgtta
      661 tagccgtccc gcaccgcctg ctcgttacca ccggctgcta tagaacccga gtcgtcgagc
      721 agcagggtca gcagaatcgc cctggtagcg ggcacgtcga gcgcgggcac gcccatcgcc
      781 gcctggatct gcgcgcccag gtcgggtacg gacagcgcct ggaacgaagc cttcgatagg
      841 tctccttcgt cgtgggcgtc ggcgaacagg ctgagcaccg acagagcctg gctactgatg
      901 tggttgccgt tgtggttgcc gttgtgactg ttactggaat ttgcgttcat ctttttctcc
      961 tttctgaagt tgccccagtg accggcgtga cggccgggtc tatacgtcgc aattgacgcc
     1021 taccgggtaa ccggcgcagc gagggcctcc ctgtagccgg gccattcggt cacgggctgg
     1081 tccgaggtca cgatgtgcat cccggcccgc tcgaagcgct ggagcgcggc ctcggcgtcg
     1141 ggggtgaagt cataaccggg ggtaacgacg ggcgaggagc aatcccggat gatgtgcacc
     1201 ttgcgggctg ccgcggggtc cttctgaagt atgtcgcgga gcagatcatc tatggtccag
     1261 gccacgcagt gagagagcgc ctggcccgtg atgccgatca cctcgaaacc agacatgggg
     1321 tcgatgagct tctccaggaa cttgtagttg cgctgggcat ttggcagcgg ggcaccaccc
     1381 gtggtggtga gtacttcggg ggctagcacc gagtagttct cggtcagcgg gtggccgccc
     1441 ttgatctcga agccggtctg cgcggcgcgt gctgcattgt ggaactccac agcctcgtgc
     1501 acgataggcg cgagcgcgtg cccgatcccg cccagcatag cgtgcggtgg ccagacgcag
     1561 agggcatact tgcccgactt ctcaagctcg gccgtgtagt gctccacgtg ccgctggaga
     1621 gccaggtagt tgccccccag cgaataggtg gcgcgggggt taaccttcca gacgccccgg
     1681 acgatgtcgt cgtggctgat gaaagtgtag ggatcggggt ggcggcccga gtcgttcacc
     1741 cagaacagcg ggtggaagac ctggtactgc gtgtgggtgt ccatggtagg gaagatccgc
     1801 gtgatgtgcg gcatgttcct gtagatgaac tcgcacagcc taatattgtc ctcgaccgcg
     1861 cccctacccg agcggccgcc cacgtacagc tcgtaaccgg ggatgcagaa ggtgatctgg
     1921 gcgtcgatca ccaggagcgc cgtgcgcacg cggtcggacg cagagggttc tatgtcatgc
     1981 cgggcggccc actcttcggc ctcgcgctcg atacgcaggt agtcgaccgg ccacaccttg
     2041 tcggccctgg aggggtcgaa gtgaggtggg atgggcagag tgggcagaag tccccctgtg
     2101 ttaccgttct gggtggttat ctgggtagcc attgtattgt cctccttatg gcccctcctt
     2161 acgagcgggc cgctaaatgg ggtgggttgc caacaccccg cccggcggcg ggacggtgtt
     2221 tccggcgttt cccagccgga gcaggtagat ggtgcggtcg ctgacggcgt agaggccgtc
     2281 gcggcccgcg aacagttggg tggcagagtc gacgtagggc gcggtgtcgg ggaataggcg
     2341 tgtttggacc agcgcccccg agagctcatc cacgcgggcc accccgtcat cggtggcgca
     2401 cagcaggctg ttaccggcag cgcacttgcc tcgcagggtg cctagccagg acccgtcccc
     2461 ctctggtgcc gcatgccgcc cgagcacatg cccgtcgcgc gccaccagga cgcagtggtt
     2521 ggtcacgccc ttgggtccct ttatggccgc aaggaaccag caccggtcgt ggccgaacga
     2581 gcacgtggcg tcgagcagtt ggccgggcgg gagcgccagg tcgacccggt cgttcacgcc
     2641 cgcatgttcc gcgtcgaaaa gcagtgcccc ctccagacca cccgcccggt agtgccccag
     2701 cccgaaccgc tcgcccgccc agatgcgggt ctggtgggag agcacgtcgc cgatagcgta
     2761 ggggccgagc gccgagtccc tgtagagccg cccgccctct acccagtaga ggtgggccga
     2821 gtttgcagcg aacgccggta tcgcggacgc gcctgcgccc gtgaccgtct cgatcacccg
     2881 ccgctccggg ggcgcacccg ctcgcaggct gaccaggttg gccccccgcg ctacgtgtgt
     2941 ggcgttaccc gacaggcgga cgcgtatacc cttgactagc ccccatcgca gcagggtcga
     3001 accgtcctcc cggcgcaact cgccgtcctg ctggtagagc cagcacagtt ggccgtctcg
     3061 cacagcggcc gccactacgc gcccctctgt cctgaatacc agggtggccg tcacatcgcc
     3121 gcgcacggtc tggaggggta ccacgggggg cacctggacg caagtctgtg gttggcacca
     3181 ggggcatgtg gggtgtgcgt ggtcggctcc gcacaggggg cagcgggacc agcgcaggtc
     3241 ctgtagcagc cggaccggga actcccccct gcggtcgccc tcaaacgtct cacggaagta
     3301 ctccagcagt tcatcgggca gggcgcgata ggggatggcc gggcgcgggt actgcacgtc
     3361 cggcgagaag accgtgatgc ggtgcagcgg ccgcgtcgac tcggatatct tgcccttact
     3421 cttgcccgcg gccttcggct tgtacagccc tgcgtacggg tctacgaaga ggaggcactg
     3481 cagggcgatc accgagtagg cgtaccagtc cgactcttga gtgtgggggc ggcttagctc
     3541 caggtggtcc agtcgcgggt cgcagatgag gggatcgatg aagcggtact ggaaagtgcg
     3601 gcacagaaag cctccgtact gaaacgagtc cgcgtccacc aggtacgtga tgtcgtctcg
     3661 aaccaggacg ttcagatcgt tgaagtcgcc gattacaacc cctgccgagt gcaccgcgcg
     3721 caccgtgccg tgcaggtcca gtaacacatc gcgcacccgc tccggggtaa gccccgtctg
     3781 tagtgcgtcc tctcggaact tacgcttccc gtactggtac agcaggtcgg ctcccgccag
     3841 gtaaggcatc gtgtaaccga ctacgcaggc ccccgtgccc ggcccgtcgg aggccaactc
     3901 aagtggtccc agcacggatg agggcaggtt gcgcgggaag acgcgcagct tgcgctgcgc
     3961 cgtgtcgagc cgctcctgcg cggctgcctt gagcgcggcc ctctcgacgg ggttggccga
     4021 ttgcaggtcg gggtggtcgg ggcttttgta tactttcagg gccagcccgc cgcctattga
     4081 gtagacatcg gcctccccac ccgtgcctat ggcctgcgcc gcctgcagcg tcagggttcg
     4141 cccgcccacg tacaccttac gcgttcccat gtgcgacctc ctcccgaggg gcgcggcgcc
     4201 ctaccacgag agtagcgtcg tcctccactc gcccccaatc ggttaacttg cgccgggcgt
     4261 cccagtcgat ccgctgacag tccgtgccca gcaggcgcag gcggttcggg agcgcggcgg
     4321 cgttacggta gtagagatcc tcacgccacc aggggtccag acctcccgcc acatcttcct
     4381 tgccc
//
LOCUS       DASRWZ010000653         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1218488, whole genome shotgun sequence.
ACCESSION   DASRWZ010000653 DASRWZ010000000
VERSION     DASRWZ010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218488"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1953
                     /locus_tag="VFH60_10360"
     CDS             229..1953
                     /locus_tag="VFH60_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314230.1"
                     /translation="MRRNPRHTQVVLRKLAVLLLLGAALPAVSLAAPQAQSGGPRASA
                     SFTLAGGAGRQLAPSAAGNVVVYSDCATGTCRVRGVKLDNKQAFSISPTAAGQLPGTD
                     GSRVVWMDGRNATDTSTTDRLNNMDVYAARLDGSGEQVVSKAPNLQSRPRVSGNIAVW
                     SDHRAATSDEDSESGDIYMYDFATGQETRVSGAASAQIRPATNGKVIVWEDYRNEPDP
                     NGFNSAIYGYDLATKQEFVITNAPDQQSDPAIWGNIVVWSDFRKGEDYNADIYGYDLT
                     TRQEFLITDAPGSQTQPAIDGYLVVWADYRNNPQPGSGNNSDIYGYDLITKREFSIFM
                     GPGVQGGAVVSGNTVAWEDFPSGDVETGESDVKAATVSGVTSAAPGGKTLPPPALLPG
                     RGSRTFPETGKTVSGAFLEYWDKNGGLAQQGFPISEVMGEVSDLNGKAYTVQYFERAV
                     FEYHPENQPPHNVLLSQLGTFQYKKKYPNGAPQPPTEPGGLTFPETGKKVSGRFLEYW
                     QQNGGLAQQGLPLSNPFQEKSDTDGKTYTVQYFERAVFEAHPEKPAPYDVLLSLLGTF
                     QYKQKYTP"
BASE COUNT          452 a          742 c          618 g          345 t
ORIGIN      
        1 gggtagcagt caagagcggg ctgctgatcc ttcgctacgc tcaggatgac agtgggaggc
       61 tttggtccgt cccaatgctg tgcatcagct actcaacggg gcacgtctct gcgcgcccac
      121 gtacaacacg accccggcca aaacatgtta cactggacgt gcggcaggca acctcgcgta
      181 gcgcccgttc gcacgtctac acacctaaaa cccgccggga gatcatacat gcgtcgtaat
      241 ccgcgccata cccaggttgt gcttcgtaag ctggctgtgt tgctgctgtt gggagccgct
      301 ttgcccgccg tgtccctggc cgcgccgcag gcacagtccg gcgggccccg ggcatcggcc
      361 tctttcacgc tggcaggggg agcgggcagg cagctagccc cctccgccgc cggtaacgtt
      421 gtggtataca gcgactgcgc cacaggcacc tgccgagtgc ggggcgtcaa gctcgacaac
      481 aagcaggcct tctccatcag tcccaccgcc gcgggccaac tgcccggcac cgacggcagc
      541 cgcgtggtct ggatggacgg ccgtaacgcc accgacacca gcaccaccga ccggctcaat
      601 aacatggacg tgtatgccgc caggctggac ggcagtggcg agcaagtcgt gagcaaagcc
      661 cccaacttgc agagccgccc ccgcgtgtcc ggcaacatcg cggtctggtc ggaccaccgc
      721 gcggccacga gcgacgagga ctccgaatcg ggcgacatct atatgtacga ctttgccacg
      781 ggccaggaga cccgagtgtc aggagccgcc agcgcgcaaa tccgccccgc caccaacggc
      841 aaggtgatcg tgtgggaaga ctaccgcaac gagccggacc ccaacggctt caactccgcc
      901 atctacggct acgacctggc taccaagcag gagttcgtta tcaccaacgc gccagaccag
      961 cagtccgacc ccgccatatg gggcaatatc gtggtctggt ccgacttccg caagggcgag
     1021 gactacaacg ctgacatcta tggctacgac ctcaccacca ggcaggagtt tcttatcacc
     1081 gacgccccgg gcagccagac ccagcccgcc atcgatggct acctggtggt gtgggccgac
     1141 taccgcaaca acccgcaacc cggcagcggc aacaactccg atatttacgg ctacgacctg
     1201 attaccaagc gcgagttctc catattcatg ggtcccggcg tgcagggtgg cgcggtggtc
     1261 tccggcaaca cggtggcctg ggaggacttc ccctccggcg acgtggagac gggcgagtcg
     1321 gacgtcaagg cggctaccgt gagcggcgtt acaagcgcgg cccccggtgg caagaccctg
     1381 cccccgcccg cgctcctgcc cggcaggggc agccgcacct tccccgaaac cggcaagacc
     1441 gtctccggcg cgttcctcga atactgggac aagaacggcg gcctcgccca gcaaggcttc
     1501 cccatctcgg aagtgatggg cgaggtgtcg gacctcaacg gcaaagccta cacggtgcag
     1561 tacttcgagc gggcggtgtt cgagtaccac cccgaaaacc agccccccca taacgtgctg
     1621 ctctcccagc taggcacgtt ccagtacaag aagaagtacc ctaacggtgc cccgcaaccg
     1681 ccaactgagc ctggcggcct cacgttccct gagaccggca agaaggtatc cggcaggttc
     1741 ctggagtact ggcagcagaa cggcggcctc gcccagcagg gcctacccct ctccaacccc
     1801 ttccaggaga agagcgacac cgacggcaag acctacaccg tccaatactt cgagcgcgcc
     1861 gtcttcgagg cccaccccga aaagcccgcc ccctacgacg tcctcctctc cctcctcggc
     1921 accttccagt acaaacagaa gtacacaccg tgagtaatgg acgaaaacgc aaagacgcaa
     1981 agacgcaaag aacgcgcaaa gaggatgaag gtttagtggg aacaaagaga accagcgtac
     2041 gtcacgatat acgctggttc tctttgtact cagaacaaca atccttaatc tctgcgcgtt
     2101 ctttgcgtct ttgcgtcttt gcgttttcgt ctctatggct gtgggcgata aggcccc
//
LOCUS       DASRWZ010000654         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_53076,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000654 DASRWZ010000000
VERSION     DASRWZ010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53076"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1402)
                     /locus_tag="VFH60_10365"
     CDS             complement(20..1402)
                     /locus_tag="VFH60_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HET6314231.1"
                     /translation="MVLQSDAPGKLSGADEASEHTPDIKGPLPGPKAQAVFDRDCAVI
                     SPSYTRSYPFVMGYGKDCMAWDVDGNRFIDFTAGIAVLATGHSHPEVVRAVQEQAAKY
                     IHMAGTDFYLPEAVDLAEALCRITPGDHEKQVFFTNSGTESIEAAMKLCRHHTGRPVL
                     ISFIGGFHGRTYGSMSLSSSKYLHRTKYQPLVGGIYHAPYPNPYRSPFPHLPTEQLGR
                     ACVDYIEKTMFRHLVSPHEVGGIFVETIQGEGGYVVPTDDFYPALRDLCDRYEIPLVL
                     DEVQSGFGRTGKMFAIEHWGIEPDVMCFAKGIASGMPMGAIVARKSIMGGWTSGAHAN
                     TYGGNALAVAASLATIRLLEEGLTDNARDMGDYILRRVNGWLDRYPFVGDVRGKGLMI
                     GVEIVKSKEGREADHDLRDRIVDESFQRGLLLLGAGPNTIRFCPPLTLNQPTADAALD
                     VMEAVMDTLR"
     gene            1695..2612
                     /locus_tag="VFH60_10370"
     CDS             1695..2612
                     /locus_tag="VFH60_10370"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HET6314232.1"
                     /translation="MRVTIYTKPGCHLCEDALDLLDKLTPQYKLVVEQANILDDMALY
                     EAMHEQIPVIVAEEGQYGRLVAPISEPELRAYLEVVRRDRRYGRSVLPDYKESVLDRT
                     ARWIGKHWLKMALVFVVVFVVLPWLAPAFAAFGWWDVANPIYTAYALTCHQLPERAGT
                     VFGYQVAFCYRNTAIYGGFIVFGALYGLARDRHVPRLQWLRKPLPWWLFAAMLLPMAA
                     DGLTHMLGLRDTMLDMTVEANFGMFTIGSQPLSLNWWLRIITGFLAACGTVWFAFPRM
                     DRAVAESEAQAQIYRMSALNYPPGMAQRV"
     gene            2662..>2953
                     /locus_tag="VFH60_10375"
     CDS             2662..>2953
                     /locus_tag="VFH60_10375"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET6314233.1"
                     /translation="MDSLAFLGLSFFAGLWSFLSPCVLPLVPAYIGYLGGSALPVARG
                     VSSAGGGQATMTQTTARGIVLANALLFVLGFTIVFAMIGNLAGALSTLLYENK"
BASE COUNT          485 a          865 c         1005 g          598 t
ORIGIN      
        1 gttttcgtct tttacaactc taacgcagtg tgtccatgac cgcttccatg acgtcgaggg
       61 cggcgtcggc ggtgggctgg ttgagggtga ggggcgggca gaagcggatc gtgttcggcc
      121 ccgcgcccag gaggagcagg ccgcgctgga acgactcgtc cacgatgcgg tcgcgcaggt
      181 cgtggtcggc ttcgcggccc tctttgctct tgactatctc gacgcctatc atcaggccct
      241 tgccgcgcac gtcgcccacg aaaggatagc ggtcgagcca gccgttgacg cggcgcagga
      301 tgtagtctcc catgtcgcgg gcgttgtcgg tgaggccctc ttcgagcagg cgtatcgtgg
      361 cgagggaggc ggccacggcc agggcgttgc cgccgtaggt atttgcgtgc gcgcccgagg
      421 tccagccgcc catgatgctc ttgcgggcca cgatagcgcc catcggcatg cccgaggcga
      481 tacccttcgc gaagcacatg acgtcgggtt cgatgcccca gtgctctatc gcgaacatct
      541 tgccggtgcg cccgaagccc gactgcacct cgtcgagcac cagcgggatc tcatagcggt
      601 cgcacaggtc gcgcagggcg gggtagaagt cgtcggtggg caccacgtag ccgccctcgc
      661 cctggatcgt ctcaacgaag atgccgccta cctcgtgcgg ggagaccagg tgccggaaca
      721 tcgtcttttc gatatagtcc acgcaggcgc ggcccagctg ctcggtcggc aggtggggga
      781 agggcgagcg gtaggggttg gggtaggggg cgtggtagat gccgccgacg aggggctggt
      841 acttcgtgcg gtgcaggtac ttgctggacg agagggacat cgagccgtag gtgcgcccgt
      901 ggaacccgcc gatgaacgag atgagcaccg ggcgccccgt gtggtggcgg cacagcttca
      961 tcgcggcttc aatcgactcg gtgccggagt tggtgaaaaa tacttgcttt tcgtggtcgc
     1021 cgggggtgat gcggcagagg gcttcggcaa ggtccaccgc ttcgggcagg taaaagtcgg
     1081 tgcccgccat gtggatgtat ttagcggcct gctcctgcac ggcgcgcacc acttcggggt
     1141 gggagtggcc ggtcgccagc acggctatac ccgccgtgaa gtcgataaag cgattgccgt
     1201 ctacgtccca ggccatgcag tctttgccgt agcccatgac gaagggataa gagcgggtgt
     1261 aagaggggga gatgaccgcg cagtcccggt cgaaaaccgc ttgcgccttg gggccgggga
     1321 gcgggccttt gatgtcgggg gtgtgttcgg aagcttcgtc cgcgccggag agcttgccgg
     1381 gtgcgtcaga ttgcaagacc atgtagaatc ctccatcgga attagggtta cgacgccgta
     1441 acccgcagca tagagcgggc tgtgaggaca gtctatcaca ccctcgcctt tgtggcaatt
     1501 ccttagtgct aaggttggag agggactcaa ggcggcgtga ggtggaagac cttaagcaaa
     1561 tgataccgac atcatcgtcc ccttgacccc ctctgccaca cgctcgtata atcttaatgc
     1621 tagttcccgt tcaataactt tcaggccggg tgccgccacg cactctctgg cctgctggaa
     1681 gccggttctt ctatatgcgc gtcacgatct acaccaagcc tgggtgccac ttgtgcgagg
     1741 atgctttaga cctcctcgat aagctgacgc cccagtacaa gctcgtggtc gagcaggcca
     1801 acatccttga tgacatggcc ctgtacgaag cgatgcatga gcagataccc gtgatagtgg
     1861 ccgaggaggg gcagtacggc aggctggtgg cccctatcag cgagcctgag ttgcgggcgt
     1921 acctggaagt ggtgcggcgc gacaggcggt acgggcggag cgtgctgccg gattacaagg
     1981 aatcggtcct ggaccgcacg gcacgctgga ttggcaagca ctggctgaag atggcgctgg
     2041 tgttcgtggt ggtgttcgtg gtgttgccct ggctggcccc ggcgttcgct gcctttggct
     2101 ggtgggacgt tgccaacccc atatataccg cctacgcgct cacctgccac cagctgccgg
     2161 agcgggcggg caccgtcttt ggctaccagg tggctttctg ctaccgcaac acggctatct
     2221 atggtgggtt catcgtcttc ggtgccctct acggcctggc ccgcgaccgc cacgtgccgc
     2281 gcttgcagtg gctccggaag ccgctcccct ggtggctgtt cgcggcgatg ctgctgccga
     2341 tggcggcaga cgggctgacc cacatgctcg gcctgcgcga tactatgctc gacatgacgg
     2401 tggaagccaa tttcggcatg ttcacgatag gctcgcagcc gctctctctc aactggtggc
     2461 tgcgtatcat caccgggttc ctggccgcgt gcgggacggt ctggttcgcc ttcccacgta
     2521 tggaccgcgc ggtggccgag tcggaggcgc aggcgcaaat ctaccggatg tcggccctga
     2581 actacccgcc cggcatggcg cagcgggtgt gagaggcagt gacagcgttg cagacgttgt
     2641 aactacatag agggaaactg tatggactcg cttgcttttt tggggctatc atttttcgcg
     2701 gggctgtggt cgttcctgtc gccgtgcgtg ctgccgttgg tgcccgccta cataggctac
     2761 ctgggtgggt cggccctgcc ggtggcgcgg ggtgtatcca gcgccggggg cggccaggct
     2821 acgatgacgc agaccaccgc ccgcggaatt gtgctggcaa acgcccttct ttttgtgctc
     2881 ggcttcacca tcgtgttcgc tatgatcggc aatcttgccg gggcgttgag caccctgctg
     2941 tacgagaaca agc
//
LOCUS       DASRWZ010000655         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1012655, whole genome shotgun sequence.
ACCESSION   DASRWZ010000655 DASRWZ010000000
VERSION     DASRWZ010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012655"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..899)
                     /locus_tag="VFH60_10380"
     CDS             complement(255..899)
                     /locus_tag="VFH60_10380"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET6314234.1"
                     /translation="MAHELPPLPYDYNALEPYIDEETMHLHHDKHHQAYINNLNTALA
                     NYPDLQDKSAEELIRDLDSVPEAIRPAVRNNGGGHVNHTMFWEIMGPNGGGEPSGALA
                     DAINQSFGSFDAFKQQFNDAGTKRFGSGWAWLVRGSDGGLSVISTANQDNPLSDGQYP
                     VLGNDVWEHAYYLKYQNRRPEDLAAWWNVVNWDVVSQRYEQAGKTRRDGTPILP"
     gene            1139..2416
                     /gene="lysA"
                     /locus_tag="VFH60_10385"
     CDS             1139..2416
                     /gene="lysA"
                     /locus_tag="VFH60_10385"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET6314235.1"
                     /translation="MAWNRQASALAYIRGRLHVEEVPVEELARRFGTPLYAYSSATLR
                     RQYARLESSMRGLVKDVLICYALKANANPEIGGLLASLGAGADVVSGGEVYLARRMGF
                     PGERIVFAGVGKTRREMSEALDAGVHSFHVESRGELEALAEVAASRGTVAPVAVRVNP
                     DVDAGTHPHITTGTRANKFGVGPAEAFDLIRWAAAAPSLAPAGLHAHIGSQLQRVQPI
                     LDAVDRLLELWDTLQAEGINLTELDIGGGLGIQYRPEEEPEGPEELAAGLWPLLAGRK
                     LHLTLEPGRFIVGPAGALITSVTYTKEVPAPGQGERPGLLAIADAGMNDLLRPALYGA
                     WHPVWPLHERDVDRGYPLDVVGPVCESSDVLARGRRIGDVKPGDLLAIGQAGAYGYSM
                     ASQYNARPRPAEVLVTGTEARLIRPRETYEDLW"
BASE COUNT          437 a          786 c          807 g          455 t
ORIGIN      
        1 acaatgccca ctcccttttg ctttgggaca atagacgata agagagacgc aaccgccaag
       61 acgccaagag cctgccctga gcgcagccga agggacgcca agggccacgc caagctacag
      121 gattgttcag ttctaagcaa agggacaaga gtacaacatc atgtactctt gtcctttcgt
      181 actcaccact ccatcttaag ctttgcgcgt tctttgcgcc tgtgcgtctt catctcttgg
      241 cgctactttg gggttcatgg cagtatgggg gttccgtctc ttctcgtctt tcctgcttgc
      301 tcgtaccgct ggctcacgac gtcccagttg accacgttcc accaggccgc aaggtcctcg
      361 gggcggcggt tctggtactt gaggtagtag gcgtgctccc agacgtcgtt gcccagcacg
      421 gggtactgcc catccgacag cgggttgtcc tggttggcgg tgctgatgac tgacaggccg
      481 ccgtcggagc cgcgcacgag ccacgcccag ccggagccga agcgcttggt gcctgcgtcg
      541 ttgaactgct gcttgaaggc gtcgaagctg ccgaagctct ggttgatcgc gtcggccagc
      601 gcgccacttg gctctccccc gccattggga cccatgatct cccagaacat ggtgtggttc
      661 acatgcccgc cgccgttgtt gcggaccgcc gggcggatcg cctccggcac gctgtccagg
      721 tcgcggatca gttcctcggc gctcttgtcc tgcaagtcgg gataattcgc aagggccgta
      781 ttcaggttgt tgatgtaagc ctggtggtgc ttgtcatgat gcaggtgcat cgtctcttca
      841 tcgatgtatg gttcgagcgc gttgtagtcg tagggcagag gcggtagttc gtgggccatt
      901 ttagtcctcc tgagtattcc tcgttgaatg tcatgtagag ctgctccggc ccattggccc
      961 tttccaagtc gcaagaggtg ggcgcagacc ggggagcgta cataccttat tgtacgcatc
     1021 cgcgtccaac cgtcaaggca cggggtcacg cgagcggggc cacgagcctg tttccacgtg
     1081 gtatactgcg cctgttgacg ctggccgcac cccctaacgc atacccagga ggcagctatt
     1141 ggcctggaac cggcaagcga gcgccctcgc ctatatacgc gggcgcttgc acgttgaaga
     1201 agtccccgtc gaggagttgg cccgccggtt cggcacgccc ctgtacgcct atagcagcgc
     1261 cacccttcgc cggcagtacg cccgccttga gagcagcatg cgcggtctcg tcaaggacgt
     1321 gctcatctgc tacgcgctca aggccaacgc caacccggaa atagggggct tgctggcgag
     1381 cctgggcgcg ggggcggacg tggtgtcggg cggtgaggtc tacctggcgc ggcgcatggg
     1441 cttccccgga gagcgcatcg tcttcgcggg tgtgggcaag acgcgcaggg agatgtcgga
     1501 agccctcgac gcgggagtgc actcgttcca cgtggaatcg cgaggcgagc ttgaggcgct
     1561 ggccgaagtg gcggcctcca ggggcacggt cgcgcccgtc gcggtgcggg tcaaccccga
     1621 cgtggacgcg ggcacccacc cacacatcac caccggcacg agagcaaaca agttcggcgt
     1681 aggtccggcg gaggccttcg acctcataag gtgggcggcg gccgctccgt cgctagcccc
     1741 cgccggtctg cacgcccata tcggctctca gctacagcgg gtgcagccca tactcgacgc
     1801 ggtcgatcgg ctgctggagc tatgggatac cctccaggcc gaaggcataa atctcacgga
     1861 actcgacata gggggcgggc tggggataca gtacaggccg gaggaggagc ctgaggggcc
     1921 ggaggagctt gccgccggac tgtggcctct gctggcgggc cgcaagctac atctcacgct
     1981 cgaaccggga cgcttcatcg tgggaccggc gggcgcgctt atcacgagcg tgacgtacac
     2041 gaaagaagtc cccgcccccg gccagggtga acgccccggc ctgctcgcaa tcgcggacgc
     2101 gggcatgaac gacctgctaa ggcccgccct gtacggtgcc tggcaccccg tgtggcccct
     2161 gcacgagcgc gatgtagacc ggggctaccc gctcgacgta gtgggtccgg tgtgcgaatc
     2221 gtccgacgtg ctggcgcggg gccgccgcat aggagacgtg aagccgggcg acctgctggc
     2281 aataggccag gcaggtgctt acggctactc gatggcctcc cagtacaacg ctcgcccccg
     2341 ccccgccgag gtactcgtca ccggcaccga agcccgcctc atccgccccc gtgagacgta
     2401 cgaagacctg tggtgaagag aggagtgccg agtgccgagt accgagacga aaacgcaaag
     2461 gacacaaaga acgcccatag cggat
//
LOCUS       DASRWZ010000656         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1287783, whole genome shotgun sequence.
ACCESSION   DASRWZ010000656 DASRWZ010000000
VERSION     DASRWZ010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287783"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VFH60_10390"
     CDS             complement(<1..817)
                     /locus_tag="VFH60_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET6314236.1"
                     /translation="MTTDIDIESRAEPAASSTNEELVDTIFNTVRRLIQERHHIPAAT
                     YRLQFNRFFTFQQARELVPYLHRLGVSDIYASPYFKARAESLHGYDIANHNELNPSIG
                     TEADLDALLAELHRHDMCHILDTVPNHMGIGEEGNTWWRDVLENGRSSLYAPYFDIDW
                     DPINTKLTNKVLLPILGEQYGKVLENRELQVTFAPEEGRFYLRYWEKTFPINPRSYQA
                     ILDYDKDRLLDELGHDSEPALEYQSILTALDHLPSHFETERERILERNREKEVI"
     gene            1345..>3636
                     /locus_tag="VFH60_10395"
     CDS             1345..>3636
                     /locus_tag="VFH60_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6314237.1"
                     /translation="MAGAITFGQWLREQRKKRDLTQEMLAERIGCSIGAIQKIEVGAR
                     RPSRQVAELLAEVFGIPPSDLQDFVRFARSREDAAEAAEAAHAPAVSAAQTASLAEPA
                     PGGQSGHAGQAPEVSLPADNLPAQLTQLVGRDSEVAEIGGRLLRDEARLLTLTGAPGT
                     GKTRLAIEVASNVKAHFEAVCFVTLSAVSDPALVLPTVSRAMGLKDAGEGTALPQLAQ
                     YIGERRVLLLLDNFEQVLGAAPHVTQLLSMCPRLKVLVTSREALNVRGEQQFQVPPLA
                     LPEHDRLPSLEQLSRYPAIALFEERARAVRPDFELSEENAEAVALICRRLDGLPLAIE
                     LAAARIRLFPPQQIAARLDSRLKLLTGGARDLPARQQTLRGAIEWSYNLLDRGEQTLL
                     SRAGVFVGGCSLSAVEAVCNAEGDLPMDVLDGLASLLDKSLVRQYEGPHGDTRFTMLE
                     TIREYAREQLDALGEGEGEVTLRMHAEYYLALAEAAEPQLIGAEQTLWLDNLESDHDN
                     VRAVLRWAIERGEAEFAARLGATLWQFWFRRGYYGEGREWMQKVLSMPQAGHTVNRVK
                     ALNVAGFLAYGQGDFAAMSLYYEEGAALARELSDSQGTAYALEGLGIVAHHKADYEQA
                     ARHFNESREIFEQVGNSWGVSNALFRLGNVALNEGRYDEARALHEQRLAMRRALGDPG
                     RMASSLLHLGEVARCRRDYDEAYTRYSESLELYRSIGVKDGIASSYHNLGHVYYHRGD
                     YKQAAEVFRKGLLLFRELGDKLSI"
BASE COUNT          660 a         1068 c         1259 g          649 t
ORIGIN      
        1 tgatgacctc tttttcgcgg ttgcgctcaa gtatcctctc ccgctcggtc tcgaagtggg
       61 agggcaggtg gtccagggcg gtgaggatgc tctggtattc gagggcgggt tcgctgtcgt
      121 gcccaagttc gtccagcagc cggtccttat cgtaatccag gatggcctgg taggagcggg
      181 ggttgatagg gaaggtcttc tcccagtagc ggaggtagaa cctgccttct tcgggggcga
      241 acgttacctg aagctcccgg ttctccagca ccttgccgta ctgctcgccc aggatgggca
      301 ggagcacttt gttggtaagc ttggtgttga tggggtccca gtcgatgtcg aaataggggg
      361 cgtagaggga ggagcggccg ttttccagca cgtccctcca ccaggtgtta ccctcctcgc
      421 ctatgcccat gtggttgggc acggtgtcga ggatgtggca catgtcatgc cggtgcaact
      481 ccgccagcag agcgtccagg tcggcttcgg tgcctatgga ggggtttagc tcgttgtggt
      541 tggcgatgtc gtagccgtgc aggctttcgg cgcgggcctt gaagtagggc gaggcgtaga
      601 tgtcgctgac gcccagccgg tggaggtatg ggacaagctc gcgggcctgt tggaaggtga
      661 agaagcggtt gaactggagc ctgtaggtag ccgccgggat gtgatgccgc tcctgtatca
      721 gtctccttac tgtattaaat atagtatcga ccaactcttc gttggtcgag ctagccgccg
      781 gttcggcccg gctttcgata tcgatatccg tggtcacaag cgccctctcc aggggatacg
      841 tacccaagca tccctgcaca cacaatagtt tcgcccacct taatgcaaga agcgggccag
      901 gaagccgaat ggaaagcggt cgccaaagca agaacggtgc caattccgct tgttgaaaga
      961 gacgaaaacg caaagacgca aagacgcaaa gaacgcgcaa agagtacgaa ttgtttagag
     1021 gatccaaaaa ggagcagggt acacgacgca gtaccctgct cctttatctt gaacaacacc
     1081 tacgtaatct tggcgcgctc ttggcgtcct tggcgtcttg gcgtttccgt ctatttacat
     1141 gttgctgttg ccgcaatact tggagtgctg gatggggcat gttaggctgt tgttgcaggg
     1201 aaaggttgtc ccggattgga ctaatcacag gcacgaaagc cgcaaaaatg ttacactgga
     1261 tgtagtaagg ctcgtttgag aacggggggc gatagtgtgc cttcgcgcgt gggtgcgacc
     1321 ggcaggtcag gagcgggaaa cgtcatggca ggcgctatta ccttcgggca gtggttgaga
     1381 gagcagcgca agaagcgcga cctgacccag gagatgctgg ccgagcggat cggctgctcg
     1441 attggggcca tacagaagat agaggtaggc gcgcgtcgcc cttcgcggca ggtggccgag
     1501 ttgctggccg aggtgttcgg catacccccc tccgacctcc aggacttcgt gcgcttcgcc
     1561 aggagccggg aggacgctgc ggaggctgcg gaggctgccc atgcgcccgc cgtgagtgcc
     1621 gcacaaaccg catccctcgc ggagcctgca ccggggggtc aaagcgggca cgccgggcaa
     1681 gccccggaag tctctcttcc cgccgacaac ctgcccgcgc agctcaccca gcttgtgggg
     1741 cgcgattctg aggtggccga gataggcggc cgcctcctgc gcgacgaagc gcgtctcctc
     1801 accctcaccg gcgcgcccgg caccggcaag acccggctgg ctatcgaagt ggcttccaat
     1861 gtgaaggcgc acttcgaggc tgtgtgcttt gtgaccctct ccgcggtcag cgaccccgcc
     1921 ctggtgctgc ccaccgtgtc gcgcgctatg ggcctgaagg acgcgggcga aggcacggcc
     1981 ctgccgcaac tggcccagta cataggcgag cgccgggtgc tcctcctgct ggacaacttc
     2041 gagcaggtgt tgggggccgc gccgcacgtc acgcagttgc tgtcgatgtg cccacgcctg
     2101 aaggtgctgg tcacgagccg tgaggcgttg aacgtgcgcg gcgagcagca gttccaggtg
     2161 ccgcccctcg cgttgccaga gcacgaccgt ctgccatcgt tggagcagtt gtcgcgctac
     2221 ccggccatcg cgctgttcga ggagcgcgcc cgcgcggtgc ggcccgactt cgagctgagc
     2281 gaggagaacg ccgaggcggt ggccctgatt tgccggaggc tggacggcct gcccctggcg
     2341 atagagcttg ccgccgcccg catcaggctc tttccccctc agcagatagc cgcccgcctc
     2401 gacagccgcc tcaaattgct caccgggggg gctagggacc tgcccgcccg ccagcagacc
     2461 ttgaggggcg cgattgagtg gagctacaac ctgctggaca ggggcgagca gaccctcctc
     2521 tcgcgcgcgg gtgtgttcgt gggtgggtgc agcctctcgg cggtggaggc ggtgtgcaac
     2581 gccgagggcg acctgccgat ggacgtgctc gacggcctgg cctcgctgct ggacaagagc
     2641 ctggtgcgcc agtacgaggg accgcacggc gacacccgct tcacgatgct ggaaacgatc
     2701 agggagtacg cgcgggagca gcttgacgcg ctcggcgagg gcgagggcga ggtgacgctg
     2761 cgcatgcacg ccgagtacta cctggcgctg gcagaggcgg cggaaccgca gcttatcggc
     2821 gccgagcaga ccctctggct ggacaacctg gagagcgacc acgacaacgt gcgtgccgtg
     2881 ctgcgctggg cgatagagcg cggagaagcc gagttcgcgg cccggctcgg cgcgacgctc
     2941 tggcagttct ggttccggcg cggctactac ggcgaggggc gggagtggat gcagaaggtg
     3001 ctttccatgc cgcaggcggg acacacggta aaccgcgtca aggcgctgaa cgtggcgggc
     3061 ttcctggcat acgggcaggg cgatttcgcc gccatgtcgc tctattacga ggagggcgcg
     3121 gccctggcga gggagcttag cgacagccag ggtaccgcgt acgcgctgga gggcctgggc
     3181 atagtggccc accacaaggc cgattacgag caggccgccc ggcacttcaa cgagagccgc
     3241 gagatattcg agcaagtagg caacagttgg ggcgtatcga acgcgctgtt ccgcctgggc
     3301 aacgtggccc tgaacgaggg caggtacgac gaggcgcgcg cactgcacga gcagagactg
     3361 gcgatgcgcc gggcacttgg ggatcccggc aggatggcaa gctcgctgct gcacctgggg
     3421 gaggtggccc gctgccgccg ggactacgac gaggcctaca cccgctacag cgagtcgctg
     3481 gagctatacc ggagcatagg cgtgaaagac ggtatcgcca gctcgtacca caacctcggc
     3541 cacgtctact accacagggg cgactacaag caggcagccg aggtgttcag gaagggcctg
     3601 ctgctcttca gggagttggg cgacaagctg agcatc
//
LOCUS       DASRWZ010000657         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1150440, whole genome shotgun sequence.
ACCESSION   DASRWZ010000657 DASRWZ010000000
VERSION     DASRWZ010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150440"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..959)
                     /locus_tag="VFH60_10400"
     CDS             complement(96..959)
                     /locus_tag="VFH60_10400"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HET6314238.1"
                     /translation="MPLTTRHSALVTRHSSLGTRHFPMIDIHTHIIPALDDGPTDMDV
                     SVAMGEIAAQEGTKVLISTSHSAEAGAVGYEGMLARLAEVRAAWASAGIDIRLELGVE
                     VFLDYETVESLRSGKLWTLAGSQYVLVEVPYTPWPAYAERALFDLQTAGYTPILAHPE
                     RYTAVQDDPNLLYRLVQRGILAQVTSAALLGEQGEKVHRTAEVLVRHGLAQFLASDGH
                     NVKTKRRMPLLREAVQAASHLIGEEAALALVTTNPSAVLENRAIEVAPEPVELRKSFL
                     GGLFGGGTREG"
     gene            complement(1047..1781)
                     /locus_tag="VFH60_10405"
     CDS             complement(1047..1781)
                     /locus_tag="VFH60_10405"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HET6314239.1"
                     /translation="MADKRNRGKEDSGAGLVAYTQPKSAAAEAYRTLRTNIQFASPDS
                     PVHTILATSTSPDDGKSTTIANLAITFAASGFATVLVDADLRRPHLHKIFGLSNDTGL
                     TTLVAELARAASITGATGSTGAPRVHHPLQPTQVDNLHVLTSGPVPPNPAEILGSQRM
                     NEVLAMLRDTAEYVLIDTPPIIAVTDAAVLAPRVDGVLLVINAGKTRRDLAVKARDML
                     QQVNAHLLGVVLNNATLDKSAYVYYG"
BASE COUNT          292 a          581 c          589 g          346 t
ORIGIN      
        1 aaggagactt cgctacttcc cagcccgtgg cttcagccac agggtgtcat gcggggcgtc
       61 actgccgggc catcacccca agccatcaca gcaccttacc cctcccgcgt cccgccccca
      121 aacagccccc caaggaacga cttccgcaac tccaccggct ccggcgcgac ctctatcgcc
      181 ctgttctcca gcacggcgga ggggttagtc gtcaccaggg ccagggccgc ctcttcccca
      241 atgaggtggg aggccgcctg taccgcttcc cgcaacaggg gcatgcgccg cttcgtcttc
      301 acgttgtggc cgtcggaggc aaggaactgg gcgagaccgt gccgtaccag gacctcggcg
      361 gtgcggtgta ccttctcacc ctgctctccg agaagggcgg cggaggtgac ctgagccagt
      421 atgccgcgct gcaccaggcg atacagcaga ttggggtcgt cctggaccgc ggtgtagcgt
      481 tcggggtggg cgaggatagg ggtgtagcct gccgtttgca ggtcgaacag ggcgcgctcg
      541 gcgtaggcgg gccagggggt gtaaggcact tcgaccagca cgtactggct gcccgccagc
      601 gtccagagct tcccagagcg caagctctcc accgtctcgt agtccaggaa gacctcgacc
      661 ccaagctcca accttatgtc aatacccgcg ctcgcccacg ccgcccgcac ctccgccagc
      721 ctcgccagca tcccctcgta ccccaccgcg cccgcctccg cgctgtggga ggtagaaatc
      781 agcaccttcg tgccttcctg cgcggcaatc tcgcccatag ccaccgacac gtccatatcc
      841 gtaggcccgt cgtcaagcgc gggtatgatg tgggtgtgga tgtcgatcat agggaagtgc
      901 cgagtgccga gtgacgagtg ccgagtgacg agtgccgagt gacgagtggt cagtggcact
      961 acgccaaaac ctcaatcatc tttgcgcgtt ctttgcgtcc tttgcgtctt tgcgtttccg
     1021 tctctctact cggcactcgg tactcgtcag ccgtaataga cgtacgcgct cttgtcgagg
     1081 gtggcgttgt tgaggacgac gccgaggagg tgagcgttga cctgttggag catgtcgcgg
     1141 gccttgaccg cgaggtcgcg gcgggtcttg cctgcgttga tgaccaggag cacgccgtcc
     1201 acgcgggggg ccaggacggc ggcatcggtc acggcgatga tgggcggggt gtcaatcagc
     1261 acgtactcgg cggtatcgcg cagcatcgcc agcacctcgt tcatgcgctg cgagccgagt
     1321 atctctgccg ggttaggcgg gacggggccg ctggtcagca cgtgcaggtt gtccacctgg
     1381 gtcggctgta gagggtgatg cacgcgagga gcgccggtgc tgcctgtggc ccctgtgatg
     1441 ctggcggcgc gagcgagttc ggccacaagc gtggtcagcc ccgtatcgtt ggagaggccg
     1501 aaaatcttgt gcaggtgcgg cctcctcaaa tcggcgtcca ccagcacggt cgcaaagccc
     1561 gaagccgcga aggtaatcgc caggttggcg atagtggtgg acttgccatc gtcggggctg
     1621 gtggaggtag ccagtatcgt gtgcaccggg ctgtcggggc tggcgaactg tatgttggtg
     1681 cgcagcgtgc ggtacgcttc agccgccgcc gactttggct gagtgtaagc caccaacccc
     1741 gctcccgagt cctctttgcc gcggttcctc ttgtctgcca atctcgtctc cttttaggaa
     1801 gttgtcag
//
LOCUS       DASRWZ010000658         4870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_259170, whole genome shotgun sequence.
ACCESSION   DASRWZ010000658 DASRWZ010000000
VERSION     DASRWZ010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259170"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4161
                     /locus_tag="VFH60_10410"
     CDS             <1..4161
                     /locus_tag="VFH60_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HET6314240.1"
                     /translation="PDPVLDASGRTKYKDLDLADALSRASAGHMLIQPQYKVTDAFTV
                     AMSIDSYEGLFKLAYSELRPDILQVLPPGRWTQEVRPNGTLEYIQPGDRRLQLRVIDI
                     KHTAEPSNGYFAEVAYYMVTLAGWLEQNGLDRDFVVVAGAVWPGSHDASALRKKDQKA
                     KSEGRFATQEELNTALEEDLEPLEFSVFAPRLRSFLQDNLKSALETPWQEFTIHVATT
                     CGHCEYLGHKWTPSTIVNDDHCIPTEERTESLSRIAQMTPGAALELREAGVTRVTDLA
                     ALTPADPVLDRHQTLKSRRIMFPARAQALGLGTVAMPTDVGTSAVMPKWSDLSIYVST
                     DFDVSSGITVAFGLRATWREPLPFGHPKDDARKEAWPAFNPGAARAQAGSALVFPVDS
                     RDMTSERRELLAFLGAMQGILDDVGNLDAQSRFATANSPVRSSMQVYIWDSLQLKHLQ
                     RVIGRHLPSILANPSISGLTWLFPPETVLPSHELQRPSPITLVQDAVASLAALPVAHY
                     YSLLRTVQVYHLPATNSDWFNVHPLYDSPLSDQIPSERAHEIWSRSTESGRHWLDQLQ
                     IYKETVAKKLHALVEVTRTLRGEIGLSLQEKAPDLQIRPPRPKGKMSFDGELWYHFSK
                     LDASIDEARVLTTRAMPPYEREARFESALLTRRLYGPEEVQALNFVQLPRRANRRVYE
                     LAPGSVAVKVKPGDFSLALSPNQDSSFLNQNCWSWLRADPTLQLLMEVAGRRRWRMEE
                     LTGVTVRAIDRERRVIVLDVSLKDGMDYVSALENAGLADFGENVSLDPVSRDYFTGKL
                     LDSLQSIGNPAIAAPEPAVLAALGQGGRRGGRPSPHTPAADFLWDARNMASSAALRPH
                     GAPFGGASSPSLDEVMARLKGLGISLTDTQWAAWRSALTTRLRLIWGPPGTGKSRTLV
                     AVVVGAFLAARIAGVPLRVLISAATYRAMDIVLKQSAEQLAKEVPLDEITVARVRSKW
                     REQPEETWAVPIDLEINPDRPSTAAIALHKRLSENAGLTLVGATPGQVHNLTRLSGDV
                     QAELFDLVLIDEASQMDVAHAIFPLCALAEGASLVLAGDPKQLPPIHQAEPPLGLESR
                     VGSIYLYMTDCKGVEQAMLDRNYRSNKTLVDFGIEAGYRSSLTSHSPDLRLALTPAAN
                     LADPSLPPPGWPAGLYWTPAWEQMMDPAVPAVCFVYRDGRSSQWNDFETETVGALLQT
                     VNGRVVNGLEGERAPDGSLLPTGTAVYTPDEFWRRAVGVVTPHRAQQSRITARLQALF
                     GPEVSNLGIIRDAVDTVERFQGQQRDIIIASYALGDPDAIRNEEEFLMSLNRFNVMAS
                     RARAKLIVLVSREVIDYLASDLAVLRESALLKLFADSYCARSTPLQLGYNDAAGKQVS
                     RPGVLKTR"
     gene            complement(4200..>4870)
                     /locus_tag="VFH60_10415"
     CDS             complement(4200..>4870)
                     /locus_tag="VFH60_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314241.1"
                     /translation="FYLPWLQLHGLVPPNGLGVEGLRENLAAKHRTDDSLQAFGKALS
                     QCFKEVARVLKPDGRLVFTYQHKTPGAWQSLGEALHGAGFRVIQLFPMLGDSSVGLHT
                     HEGSIRWDAVFVARKTETENSSDSPQGPLTLDPAAQYVAQEHYRKWVTRLERTCGDDF
                     HSGDSVNFHRACAVAAALGLNKVCSTTPTADEGYTSGIGVGESRKEKPLREALQEVPI
                     TAAV"
BASE COUNT          949 a         1539 c         1471 g          911 t
ORIGIN      
        1 ccggacccgg tactcgatgc ctccggccgc accaagtata aagacctcga tctggctgat
       61 gccctctctc gggcaagtgc cgggcacatg ctcatacagc cgcagtataa ggttaccgac
      121 gccttcacgg tggcaatgag catcgacagc tacgaaggcc tgttcaagct ggcgtacagc
      181 gaactgcgcc ctgatattct ccaggtgctg ccccctggtc gctggacgca ggaggtgcgg
      241 cctaacggca ccctcgaata tattcagccc ggcgacaggc gcttgcagtt gcgggtgata
      301 gatataaagc acaccgccga gccgtccaac ggctatttcg cggaagttgc ctactacatg
      361 gtaacgctgg cgggctggct cgaacagaac gggctggaca gggattttgt ggttgtagcc
      421 ggcgcggtgt ggccggggtc ccacgatgcc tcggccttgc gcaagaaaga ccagaaggcc
      481 aagtctgagg gccggttcgc cacccaggag gagctgaata cggccctgga ggaggacctg
      541 gagccgctgg agttctcagt atttgccccg cgcctacgca gctttttgca ggacaatctc
      601 aaaagcgccc tggaaactcc ctggcaggag tttacgatac acgtagccac cacgtgcggg
      661 cattgcgagt acctggggca caagtggacc cccagtacga tcgttaatga cgaccactgt
      721 attccgacag aggaacgtac ggagagcctg agccggatcg cccagatgac cccgggcgcg
      781 gctctggaac tgcgcgaggc gggagtcacc cgggtgaccg acctggccgc cctcaccccg
      841 gcagacccgg tgctcgaccg gcaccagacg ctcaagtcga ggcgcatcat gttccccgcc
      901 cgcgcccagg ccctcgggct gggcactgtc gccatgccca cagacgtcgg cacctcggcc
      961 gttatgccca agtggtcgga cctcagcatc tatgtgtcca ccgacttcga tgtgagcagc
     1021 ggcataactg tggccttcgg cctgagggcg acctggcgag agcctctacc gttcggccat
     1081 cccaaagacg atgcccgcaa agaagcgtgg cccgctttca accctggcgc tgcccgcgcc
     1141 caggccggca gcgccctggt attcccggtg gacagcaggg acatgacgtc ggagcgccgg
     1201 gagttactgg cctttctggg ggctatgcag ggtatcctag atgacgtggg caatctcgat
     1261 gcccagagcc ggtttgcaac ggccaacagt ccggtacgct cctccatgca ggtgtatatc
     1321 tgggactcct tgcaactcaa gcacctgcaa agggtcatag gccggcacct gccctctatc
     1381 cttgccaacc ccagcatatc ggggttgacc tggttgttcc cgcccgagac ggtgctgccc
     1441 agccacgagc tacagagacc ctctcccatt accctcgtcc aggatgccgt ggcctccctg
     1501 gccgccctgc cggtggcgca ttactactcg ctgctgcgca ccgtgcaggt gtaccacctc
     1561 cccgccacca actccgactg gttcaacgta caccctctct acgacagccc cctctcggac
     1621 cagatccctt ccgagcgggc gcacgagata tggagtcgct ccaccgaatc gggcaggcac
     1681 tggctggacc agctacagat ttacaaagaa acggtcgcca agaagctgca cgccctagtc
     1741 gaggtaaccc gcaccctgcg aggcgagata ggactctcgc tgcaggagaa ggcccccgac
     1801 ttgcagatac gcccgccccg cccgaagggc aaaatgagct tcgatggcga gttgtggtac
     1861 cacttttcca agctggatgc gagcatagac gaggcaaggg tccttaccac ccgcgccatg
     1921 cccccctacg agcgggaggc gcggttcgag agcgccttgc tgacccggcg cttgtatgga
     1981 cccgaggaag tacaggccct caactttgtg cagctcccgc gtcgagctaa tcgccgggtt
     2041 tacgaacttg ctccggggtc ggtggcggtc aaggtaaaac cgggggactt cagtctcgcc
     2101 ctctctccga accaggatag cagtttcctc aaccagaact gctggtcctg gctgcgggcc
     2161 gaccccaccc tgcagctgct catggaagtg gcggggcgca ggcggtggcg catggaggaa
     2221 cttacaggcg tgaccgtgcg ggctatagac agagagcgcc gcgtgatcgt cctggacgtg
     2281 agcctgaaag acggcatgga ctacgtatcg gcattagaga atgcaggcct ggccgatttc
     2341 ggtgagaatg tgtctctcga cccggtaagt cgtgattatt tcactggcaa gctcctcgac
     2401 tctctacaga gcataggcaa ccccgctatc gccgcgcctg aacccgcggt gctcgcggcc
     2461 ctcggccagg gcgggaggcg aggcggacga cccagcccgc atacccctgc cgccgatttc
     2521 ctgtgggacg cgcgcaacat ggcatcctcg gctgccctcc ggccccacgg cgcacctttc
     2581 ggcggggcta gttcgccctc gctggacgag gtaatggctc ggctgaaagg cctgggcatc
     2641 agccttaccg acacacagtg ggcggcctgg aggtcggccc ttaccactcg cttacggctt
     2701 atctgggggc ctcccggtac gggcaagtcc cgaaccctgg tggctgtagt cgtgggcgct
     2761 ttcctggcgg cccgtattgc cggggtgccg ctgcgcgtgc tgatatcggc cgccacgtac
     2821 cgggccatgg atatagtgtt gaagcagtcg gccgagcagc tggccaaaga ggtccccctg
     2881 gatgagatca ccgtggcccg cgtgcggtcc aaatggagag agcagccgga ggagacctgg
     2941 gccgtcccta ttgatcttga gataaaccct gataggccct cgactgcggc gatagccctg
     3001 cacaagcgcc tctctgagaa cgcgggcctt accctggtgg gcgcaacgcc cgggcaggta
     3061 cataacctca ccaggctatc gggggacgtg caggcagagc tgttcgacct ggtgcttata
     3121 gacgaggcgt cacagatgga cgtcgcgcac gccatattcc ctctatgcgc cctcgcagag
     3181 ggtgcgagcc tggtgctggc gggcgacccc aagcaactgc cgcccataca ccaggccgag
     3241 ccaccccttg ggctggagtc ccgggtaggc tctatttacc tctatatgac cgactgcaag
     3301 ggcgtggagc aggcgatgct cgaccgcaac taccggtcta ataagaccct ggtcgatttc
     3361 ggcatagaag ccgggtaccg gtcgagcctc acaagccact cgccggattt gcgactggcc
     3421 ctcaccccgg ccgcgaacct ggcagacccc agcctacccc cgcccggctg gcccgcaggt
     3481 ctatactgga cccccgcgtg ggagcagatg atggacccgg cagtgcccgc agtgtgcttt
     3541 gtctaccgag acgggcggag cagtcagtgg aacgatttcg aaacggagac cgtaggtgcc
     3601 ctgctgcaaa cggtcaacgg cagggtggtg aacggattag agggggagcg tgcgcccgac
     3661 ggcagcctac tacccaccgg caccgccgtg tacacaccgg atgaattctg gcgtagagct
     3721 gtgggggtgg tgacgccgca ccgggcgcag cagagtcgca tcacggcgcg gcttcaggct
     3781 ctgttcggtc ccgaggtaag caatctcggc attatcaggg atgccgtaga tacggtcgaa
     3841 aggttccagg gccagcagcg ggatataatc atagcttctt atgcgctggg ggatcccgat
     3901 gctatccgca acgaggaaga gttccttatg agcctcaatc gctttaacgt gatggcgtcc
     3961 cgcgctcggg ccaagctcat agttttagta tcacgcgagg tcatcgacta cctggcgagc
     4021 gacctggccg tgctgcggga gtcggcacta ctcaagctgt tcgccgattc atattgtgcc
     4081 aggagcacgc ccttacaact ggggtataac gacgctgccg gtaaacaagt ctcaaggccc
     4141 ggtgtgctca agacgaggta gctataccgc tgcccaaaag aaacggtgtt ccttgcatgt
     4201 caaacagccg ctgtgatagg tacttcttgt agtgcctctc tcagcggctt ctcctttcga
     4261 ctctcaccca ccccgatgcc actggtgtag ccttcgtcgg cggttggtgt agtgctacat
     4321 accttgttga gacctaaggc cgccgccaca gcacaggctc tatggaaatt gacactatcg
     4381 cctgaatgga aatcgtcacc acaggttctt tccagcctgg tcacccactt acgataatgt
     4441 tcctgagcca cgtactgtgc cgcagggtcg agcgtgaggg gtccttgtgg gctatccgac
     4501 gagttctcag tctcggtttt cctggcgacg aaaacggcgt cccagcggat gctcccttca
     4561 tgggtatgca accctactga gctatctccc agcattggaa ataactggat tactcgaaat
     4621 ccagctccat ggagcgcctc ccccagggac tgccaggctc caggggtttt gtgctggtac
     4681 gtaaagacga gcctgccgtc aggcttcagt acgcgtgcga cctctttgaa acattggctc
     4741 agtgctttgc caaaggcctg cagagagtcg tcggtgcgat gctttgcagc cagattctcc
     4801 cgcaagccct ccactcccag accattcgga ggaaccagtc cgtgcaattg cagccagggc
     4861 aagtagaagt
//
LOCUS       DASRWZ010000659         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_739499, whole genome shotgun sequence.
ACCESSION   DASRWZ010000659 DASRWZ010000000
VERSION     DASRWZ010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739499"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="VFH60_10420"
     CDS             <1..1510
                     /locus_tag="VFH60_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HET6314242.1"
                     /translation="VHGMIASKGVLTARGGATSHAAVVARQFGKVCVAGAENGGLHVN
                     DEDGHFTANGQTVREGDIISIDGTTGEVFLGAIALIEPNFAEEHDLLKLLSWADDVRK
                     LGVWANADYPRDAIKAVNYGAEGIGLCRTEHMFFEEERLPVVRQMILSAGEATRLDGT
                     LYDAQTALDKLGPNDDDKRPAAEKELAWAKEAADSSDAVKDYHDALAQLEVIQQGDFY
                     GILKAMEGKPTVIRLLDPPLHEFLPSYEDLLVETTEMRMRGETDSDEYREKVQLLGVV
                     GQMREQNPMLGLRGCRLGLMYPAINDMQVRAILKASLQLLNEGLESHPEIMIPLVGHV
                     NELKRVREQLEEVAKSVEAEAGRDIPYKFGTMIEIPRAALTAAQIAEHAEFFSFGTND
                     LTQTTFGYSRDDAEGKFLLQYVEQGVLPENPFQVLDREGVGQLVRMATEQGRKTRPNL
                     KVGICGEHGGDPSSIQFCHSVGLDYVSCSPYRVTVARLAAAQAALESVEKDK"
BASE COUNT          324 a          471 c          496 g          238 t
ORIGIN      
        1 cgtacacggc atgatagcct cgaagggcgt gctcaccgca aggggcggcg cgacttctca
       61 cgccgcggtg gtggcccgcc agttcggcaa agtgtgcgtg gcgggcgcgg agaacggcgg
      121 cctgcacgtc aacgacgaag acggccactt caccgccaac ggccagacgg tgcgcgaggg
      181 cgacattata tccatagacg gcaccaccgg cgaggtcttc ctgggcgcaa tcgctctcat
      241 cgagccgaac ttcgccgagg agcacgacct gctcaagctg ctgagctggg cggacgacgt
      301 tcgcaaattg ggcgtgtggg ccaacgccga ctacccgcgc gacgctatca aggcggtcaa
      361 ctacggcgcg gaggggatag gtctgtgccg caccgagcac atgttcttcg aggaggagcg
      421 cctgccagtc gtgcgccaga tgatcctgtc ggcgggcgag gctacccgcc tggacggtac
      481 gctgtatgac gcccaaaccg ccctcgataa gctggggcca aacgatgacg acaagcgacc
      541 agccgccgag aaggaattgg cgtgggcaaa ggaagccgcg gacagttccg atgcggtgaa
      601 ggactaccat gacgcgcttg cccagctgga agtgattcag caaggcgact tctacggcat
      661 cctcaaggcg atggagggga agccgaccgt gatccgcctg ctcgacccac cattgcacga
      721 attcctcccc tcatatgagg acctgctggt agagaccacc gagatgcgta tgcgtggtga
      781 gacggacagc gacgagtacc gcgagaaggt gcaactgctg ggcgtagtag gccagatgcg
      841 cgagcagaac ccgatgctcg gcctgcgcgg ctgccgcctg gggctgatgt acccggccat
      901 caacgatatg caggtgcgcg ccatcctgaa ggcgtccctc caattgctga acgaggggct
      961 ggagtctcac cctgagataa tgataccgct ggtggggcac gtcaacgagt tgaagagggt
     1021 gcgcgagcag cttgaggagg tcgcgaagag cgtggaagcg gaggccgggc gcgacatccc
     1081 ctacaagttc ggcacgatga tagagatacc ccgcgccgcc ctcactgccg cgcagatagc
     1141 cgagcacgct gagttcttct ccttcggcac caacgatctc acgcagacca cgtttggcta
     1201 ctcgcgggac gacgccgagg gcaagttcct cctgcaatac gtggagcagg gcgtcctccc
     1261 cgaaaacccc ttccaggtgc tggatagaga aggggttggg caactggtga gaatggcaac
     1321 cgagcaaggc cgcaagaccc gtcccaacct gaaggtgggc atctgcggtg agcatggcgg
     1381 cgacccctcc tccatccagt tctgccacag tgtcggcctg gactacgtaa gttgcagccc
     1441 ctaccgcgtc acagtagcgc gcctcgccgc cgcccaggcc gccctcgaat ctgtcgagaa
     1501 ggataaatag ttacgctaac cgcaaagac
//
LOCUS       DASRWZ010000660         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_122918, whole genome shotgun sequence.
ACCESSION   DASRWZ010000660 DASRWZ010000000
VERSION     DASRWZ010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122918"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2787)
                     /locus_tag="VFH60_10425"
     CDS             complement(<1..>2787)
                     /locus_tag="VFH60_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6314243.1"
                     /translation="EPRIFDDPRWPQALRDDLHTRVEDGYRRAEEMLKPTPYPANFPP
                     LPKLQALEKSAEGMGEKFYRPPINVTFKEGVNHVGVEQHACKLCGDCCSGCNYAAKNT
                     VLMNYLPDAWNHGAEIFTQVSVRRVEREGKRWLVHYSLLDTGRERFDAPTLCVEADVV
                     VLAAGTLGSTEILLRSKAAGLAISDRIGHHFSGNGDVLAFGYNNDVEINGTGFGQHPE
                     AAKGPVGPCITGIIDVREQDVLNDGYVIEEGSIPSPLATILPGALATAATISGIDTDT
                     GARDRVREKVREGQSLLLGPYRGAVRNTQTYLVMTHDDSAGRMYLDDDRLRISYPGVG
                     KQPIFERVDKSLEDATRVLGGTYVRNPVWSDLLGKDLVTVHPLGGCSMSEDAEGGVVN
                     HKGQVFLGPSGTDVYEDLYVADGSVVPRSLGVNPLLTISAVAERCCALIAQDRAWHRD
                     YTLPSKPSRPPESHRLGIQFTEKMRGYFGMGALDSYEEGAEQGKLNGSPLQFTLTITG
                     DDLDAMLKGEDHRAGIVGTVEAPALSPEPLTVEGGEFNLFVSDPTGASTRLMRYRMKL
                     RSEEGRTYYFDGFKRVHNDPGLDVWSDTATLYITLYEGESEHGPALGKGILRIHPADF
                     MRQMTTMKVTNARGIEERLEATARFGTYFAGMLFEVYGGVFARPFAPNRLPGGDTPRK
                     KRELRVDAPETHSFTTADGVQLRLTRYRGGAKGPVMLAHGLGVSSLIFSIDTIGTNLL
                     EYLFANGYDVWLLDYRASIALPTSTAPQNADDVALMDFPAAVRYIREAAGVESVQVVA
                     HCFGAVAFSMAMLGGLRGVRSAVLSQVGMHTVTPILGRAKAGLHLPSMLENLGVESLT
                     AYAGRNPDWRDRLFSRALEILPGKKEEECDNPVCPRITFMYGYLYRHSRLNPATHNAL
                     REMFGVASIDSFEHI"
BASE COUNT          466 a          889 c          880 g          552 t
ORIGIN      
        1 tatgtgctcg aacgagtcta tcgaggctac accgaacatc tcgcgcaggg cgttgtgggt
       61 cgccgggttg agcctgctgt ggcggtagag gtagccatac atgaaggtga tgcgcgggca
      121 gaccgggttg tcgcactcct cttctttctt cccaggcaga atttcgagcg cccggctgaa
      181 gaggcggtct cgccagtcgg ggttgcgccc cgcgtaagcc gtgagcgact cgacgcccag
      241 gttctcaagc atggaaggga ggtgcaatcc agccttcgcg cggcccagga taggcgttac
      301 cgtgtgcatg cccacctgcg agagaacggc tgagcgcacc cctctcaacc ctcccagcat
      361 cgccatactg aaggcaaccg cgccgaagca gtgggccacc acctgcacac tctccactcc
      421 cgccgcttcc cgtatgtaac ggaccgccgc cgggaagtcc atgagcgcca cgtcgtcggc
      481 gttctggggc gctgtggacg tgggcagggc gatgctggcg cggtagtcga gcagccacac
      541 gtcgtagccg ttggcgaaca ggtattcaag gaggttggtg cctatcgtgt ctatggagaa
      601 gatgaggctc gacacgccga ggccgtgcgc cagcatcacc ggccccttcg cgccgcccct
      661 gtagcgagtg agcctgagtt gcaccccgtc ggcagtagtg aaagagtggg tctcaggcgc
      721 atccacccgt aactcccgct ttttgcgtgg cgtgtcccct cccggcaacc tgtttggggc
      781 aaatggcctg gcgaagaccc ccccgtagac ctcgaagagc atacccgcga agtaagtgcc
      841 gaaccgggcc gtcgcctcaa gtcgctcctc tatccccctg gcgttcgtga ccttcatggt
      901 ggtcatctgg cgcatgaagt ccgccgggtg tattcgcaat atccccttgc ccagcgccgg
      961 gccatgctcg ctctcacctt cgtacagggt gatatagagt gtggccgtgt cggaccacac
     1021 gtcgaggccg gggtcgttgt ggacccgctt aaacccgtca aagtagtaag tgcggccctc
     1081 ctccgacctt agcttcatgc ggtagcgcat cagccgtgta cttgcgccgg ttgggtcgct
     1141 cacaaagagg ttgaactcgc cgccctctac cgtcaacggc tccggcgaaa gagcgggcgc
     1201 ttctaccgtg cccactatcc ctgcccggtg gtcctctccc ttgagcatgg cgtccaagtc
     1261 gtcgccggtg atggtgagcg tgaattggag gggcgagccg ttgagcttgc cctgctctgc
     1321 cccctcctca tagctgtcca gcgcgcccat gccgaagtag ccccgcatct tctcggtgaa
     1381 ttgtatcccc aggcggtggg attcaggagg gcgcgagggc ttcgatggga gggtgtaatc
     1441 tctgtgccac gcgcggtcct gggcgatgag ggcgcagcaa cgctccgcca cagccgagat
     1501 ggtcagcaga gggttgaccc ccagcgagcg cgggacgacc gagccgtcgg ccacgtacaa
     1561 atcttcatat acgtctgtgc ccgaaggacc caaaaagacc tgccccttgt ggttgaccac
     1621 gccgccctct gcgtcttcgg acatggagca gccgcccagc gggtgtaccg tcacgaggtc
     1681 tttgcccagc aggtcagacc ataccgggtt gcggacgtat gtgccgccca ggacgcgggt
     1741 cgcatcctca agactcttat ctacccgctc gaagataggc tgcttgccta cgccgggata
     1801 ggagatgcgc agcctgtcgt cgtccaggta catacgcccg gcgctgtcgt cgtgcgtcat
     1861 aaccaggtag gtctgcgtgt tcctcaccgc gcccctgtac ggcccgagca ggaggctctg
     1921 cccctcacgc accttctccc tgacccggtc ccgcgcccct gtgtcggtgt caatgccgga
     1981 tatggtggcg gcggtagcca gagcgcccgg caggattgtg gcaagcgggc tgggtatgga
     2041 gccttcctca atcacgtagc cgtcgttgag aacgtcctgc tcacgcacgt ctatgatgcc
     2101 ggtgatgcac ggcccgaccg gaccctttgc cgcctccggg tgctggccga acccggtgcc
     2161 gttgatttcc acgtcgttgt tgtagccgaa ggcgagcacg tcgccgttgc ctgagaaatg
     2221 gtgcccgata cggtccgaaa tggcaagtcc ggccgccttt gagcgcagca gtatctccgt
     2281 agagccgagc gtgcccgcag ccaggactac cacgtcggcc tcgacgcaca gggtgggcgc
     2341 gtcgaacctt tctcggcccg tgtcgagcag gctgtaatgc accagccacc gtttcccttc
     2401 gcgctctact cggcgcacgg atacctgcgt gaatatctcc gcgccgtgat tccaggcgtc
     2461 gggcaggtag ttcatgagga cggtattctt ggcggcgtag ttgcagcctg agcagcagtc
     2521 gccgcacagc ttacatgcgt gctgctccac cccgacgtgg ttgacgcctt ctttgaacgt
     2581 gacgttgatg ggtgggcggt agaacttctc gcccatgccc tccgccgatt tttcgagggc
     2641 ctgtagcttg ggtaggggcg ggaaattagc ggggtaaggc gtgggcttga gcatctcttc
     2701 agctcgccgg tagccatcct caacgcgcgt atggaggtcg tcacgtagtg cctggggcca
     2761 gcgtgggtcg tcgaagatgc gcggctc
//
LOCUS       DASRWZ010000661         1685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1288256, whole genome shotgun sequence.
ACCESSION   DASRWZ010000661 DASRWZ010000000
VERSION     DASRWZ010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288256"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFH60_10430"
     CDS             <1..807
                     /locus_tag="VFH60_10430"
                     /inference="COORDINATES: protein motif:HMM:NF039385.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HET6314244.1"
                     /translation="MALAYFLGTALVLLVRYLTQIAQNGAPLFQLPQGYSAFTANSDS
                     TLQTIIFMLAIAGLVGLFVASKLRSISGRYLYAANLRSPFWSSIIAASKEEKAMGRIV
                     AAVSMALRERSGTSAPTAQTPAEPEVYYQDENAVSINDTSLEVQGKSFPVSSLHFATA
                     YPVVGQSIRDTFTSTGLVGLAFGALNGISSDPQVREVPWVPLIIYLLAGAAISGFLFL
                     VNKRKGGPLAIIHVCWVRTAREDTVAFTSIDQGYTEQVVRAINETVHHRR"
     gene            896..>1685
                     /gene="panC"
                     /locus_tag="VFH60_10435"
     CDS             896..>1685
                     /gene="panC"
                     /locus_tag="VFH60_10435"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET6314245.1"
                     /translation="MKVIATIQELRDARAGLPQGRTLGLVPTMGYLHAGHISLVDMAR
                     RECDLVAVSIFVNPTQFGPREDFSRYPRDTERDLAMLRDAGASWVFMPGVEEIYPPGF
                     STYVQVSEVTRRLEGEVRPGHFEGVATVVAKLFNLVQPHRAYFGQKDAQQVVVIKKMV
                     LDLNFPLEIRVGQTLREPDGLAMSSRNVYLNPEERQAALVLYRALTAARELWGGGERR
                     GTPLREAMSRALAAEPLARPDYVSVANPLTLEELDDSGVAQEALA"
BASE COUNT          327 a          573 c          507 g          278 t
ORIGIN      
        1 atggccctcg cctacttcct gggcaccgcg ctcgtactgt tagtacgcta cctcacccaa
       61 attgcccaga acggcgcgcc cctcttccag ctaccccagg gctacagtgc tttcaccgcc
      121 aacagcgaca gcaccctcca aactatcatc ttcatgctgg ccatcgccgg gctagtgggg
      181 ctgttcgtgg caagcaagct gcgctcgatc agcgggcgct acctgtacgc cgccaaccta
      241 cgcagccctt tctggagcag catcatcgcc gcctcgaaag aggaaaaagc aatgggccgg
      301 atagtagccg ctgtgagtat ggccctgcgc gagaggtcgg gcaccagtgc ccccacagcc
      361 caaacgcccg ccgaaccgga ggtctactac caggacgaga acgcggtgag catcaacgac
      421 acctcactgg aagtgcaggg caaaagcttc cccgtatcga gcctgcattt cgccacggcc
      481 tacccggtcg tcggacagtc gatacgcgac accttcacga gcaccgggct tgtgggcctg
      541 gctttcggcg cgctcaacgg cataagcagc gacccgcagg tgcgggaggt gccctgggtg
      601 ccgctcatca tctacctgct ggcgggcgcg gctatctccg gcttcctctt cctggtgaac
      661 aagcgcaaag gcggccccct ggctataata cacgtgtgct gggtgcgtac cgcccgcgaa
      721 gacaccgtcg ccttcacctc catcgaccag ggctacaccg agcaggtcgt ccgcgccatc
      781 aacgaaacgg tacaccaccg gaggtgaatt tggaatttcg aatttcggat tgcgaattag
      841 gggagctacc cgtaacgtac gtaatccgaa attcgtaatc cgaaattcga aacacatgaa
      901 agtgatcgcc acaatccagg agcttaggga cgcacgcgcc gggctgccgc agggtcggac
      961 gctcggcctg gtgccgacaa tgggctacct gcacgcgggc cacatctcgc tggtggatat
     1021 ggcgcggcgg gagtgcgatc tcgtggccgt aagcatattc gtgaacccca cgcagttcgg
     1081 ccccagggaa gacttcagcc gctatccacg cgacacggag cgcgacctgg caatgctgag
     1141 ggacgcaggc gcgagctggg tcttcatgcc cggcgtggag gagatatacc cgccgggctt
     1201 ctccacctac gtgcaggtga gcgaagtcac ccggaggctt gagggggaag tgcgtcccgg
     1261 ccacttcgag ggggtcgcca cggtggtcgc caagctgttc aacctggtgc agccccaccg
     1321 cgcctacttc ggccagaagg acgcccagca ggtcgtcgtc atcaagaaga tggtgctcga
     1381 cctgaacttc ccgctcgaga ttcgcgtggg gcagacgttg cgcgagccgg acggcctggc
     1441 catgtcctcc cgcaacgtct acctcaaccc ggaggagcgc caggcggccc tcgtcctcta
     1501 ccgcgccctc accgccgccc gcgagctttg gggcggcggc gagcgccggg gcacccctct
     1561 gcgagaggca atgtcccgtg ccctcgccgc cgaacccctg gcccgccccg actacgtaag
     1621 cgtagccaac cccctcaccc tggaagagct ggacgacagc ggggtagctc aagaggccct
     1681 ggcct
//
LOCUS       DASRWZ010000662         1830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1150882, whole genome shotgun sequence.
ACCESSION   DASRWZ010000662 DASRWZ010000000
VERSION     DASRWZ010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150882"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFH60_10440"
     CDS             <1..418
                     /locus_tag="VFH60_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HET6314246.1"
                     /translation="RGQASKIVSNTFFPNCQDPVPTSTPVPTNTTMPTSTPMADLDVM
                     IKNFDYAPKHITVTMGTTVRWTNFDLDYHTVTSCVRDPKTESCSPSGAFDSGNIEQNE
                     QWQYTFSSLSPGLYEYYCIPHPYMTGTVTLLPVLVR"
     gene            723..>1830
                     /locus_tag="VFH60_10445"
     CDS             723..>1830
                     /locus_tag="VFH60_10445"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET6314247.1"
                     /translation="MAKRKSLLIFGLVGLLAVVTALSFAASGRSGTAAGNTNGLTVPA
                     ANMSFAANFKAQPAAGAPVSLPWANTKVNTDATNEAQNEPFVAINPNNSQHMVIGANN
                     WVVGNGHYEVSAYVTFDGGQTWAASHPYIDRNASRINAADPTVAFGADGKVYFAFVAM
                     YPANGAVAVSTSNDGGLTWSFQSWATSFTVGADKPAIAYANGKLHVFYQNNSLYGRVS
                     SDGGSTWGNATLIESGGRNAAPVVDRGNVVLFYNTASAIKMARPNTARGGGYTISTVA
                     ATTALQPRPTHYRAGIYPAAGADGKGNLYVAWADGRNVGRGNDILISRSTDGSSWSAP
                     VAVNTDATAADQLMPALAVSRDGAVTVAWLDNRND"
BASE COUNT          393 a          626 c          492 g          319 t
ORIGIN      
        1 caggggccag gcgtcgaaga tagtgagcaa caccttcttt cccaactgcc aggaccccgt
       61 gcccaccagc acgcccgtac ccaccaatac gactatgcct acctctacgc cgatggcgga
      121 cctggacgtg atgataaaga acttcgatta cgctccgaag cacatcaccg tcacgatggg
      181 cacaacggta cgctggacca acttcgacct cgactaccac accgtcacca gttgcgtccg
      241 cgacccgaag acggagtcgt gcagcccatc cggcgcgttc gacagcggca acatcgagca
      301 gaacgagcag tggcagtaca cgttcagctc gctcagccca ggcttgtacg aatactactg
      361 catcccccac ccttacatga cgggcaccgt cacgctactg ccggtgttgg tgcgctgagc
      421 aggtagcaat caagtaaaaa ccagcaagaa gtcgggtctc acgcccggct tcttgctttt
      481 cctgtgtaag tgttcagatt caagatacta tttctccaca cacggatata ctttttcctg
      541 ctcaaaatac ttccatttag gcgggagata tgctaagata agcaggcaga gcgccacacc
      601 ttcctcccta tttcccactg tggcttgcaa cgccacacga agaaccgctc accccccggc
      661 acacctgttc tgcggcgaca catgttccct gtattttcta aagcaagaga ggagaaaata
      721 gtatggcaaa gcgcaagtca ttattgatct ttggcctggt aggtctgctc gcggtggtga
      781 ccgcgctctc cttcgcggca tccgggcgct caggcacggc ggcaggcaac acgaacggct
      841 tgacggtgcc cgccgccaat atgtcgttcg ccgctaactt caaggcccag cccgcagcag
      901 gcgcacccgt tagcctcccc tgggccaaca ctaaggtgaa caccgacgcc actaacgaag
      961 cgcagaacga gccgttcgtc gccatcaacc ccaacaactc gcagcacatg gttatcggcg
     1021 cgaacaactg ggtcgtgggc aacgggcatt atgaagtttc cgcgtatgtg accttcgacg
     1081 gcggccagac ctgggctgcc tctcacccct acattgaccg caacgccagc cgcatcaacg
     1141 ccgccgatcc gacggtagcc ttcggggcgg acggcaaggt atacttcgcc ttcgtcgcca
     1201 tgtaccccgc taacggcgcg gtggccgtca gcacctcgaa cgacggtggc ctcacgtggt
     1261 cgttccagag ttgggcaacc agcttcacgg tcggcgctga caagcccgcc atcgcgtatg
     1321 cgaacggcaa gctccacgtc ttctaccaga acaactcgct ctatggccgc gtctcctccg
     1381 acggcggctc aacctggggc aacgccactc tgatcgaatc gggtgggcgc aacgcggccc
     1441 cggtcgtgga caggggcaac gtggtcctct tctacaacac ggccagcgcc atcaagatgg
     1501 cccgcccgaa taccgccagg ggcgggggtt acaccatctc gacggtcgcc gccaccaccg
     1561 ccctacagcc ccgccccacg cactatcgcg cgggcatcta cccggcggca ggcgcggacg
     1621 gcaagggtaa tctctacgtg gcatgggcag atggccgcaa tgtcggtcgc ggcaacgaca
     1681 tcctcatcag ccgctcgaca gacggctcaa gctggagcgc gccggttgcc gtcaacactg
     1741 acgccacagc cgccgaccag cttatgcccg ccctggcagt cagccgcgac ggcgcagtga
     1801 ccgtagcctg gctcgacaat cgcaacgacg
//
LOCUS       DASRWZ010000663         5023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_191682, whole genome shotgun sequence.
ACCESSION   DASRWZ010000663 DASRWZ010000000
VERSION     DASRWZ010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191682"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFH60_10450"
     CDS             <1..860
                     /locus_tag="VFH60_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314248.1"
                     /translation="FRAADQEYESAAAIENTLAKLDEGRQSISQAGKPPTKRGQVGFR
                     RQSGGDSANSNEGAATPGGQDTGGAGADSEGADNTAPYNSSNVGGNAASFGGGQPGSP
                     ANGGQPGQQPSSGGGPPGGPNSDVAGGTAGGGQDDGSKGTLQGEITGPVGGAGGAISR
                     VQNPAGQGEGGSSAPASEDNPPPDNEQVYVPSQEGAPSSAEEPSVAGQAAPEPPNTDG
                     LEGRVGEGGGGEERRGQSGPGTLAPIHTPYKEVIGQYAERATESLDRAYIPPDAKDYV
                     REYFTQLGK"
     gene            971..1963
                     /locus_tag="VFH60_10455"
     CDS             971..1963
                     /locus_tag="VFH60_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6314249.1"
                     /translation="MPEITPEQFRDKAIRIEHEIGKVIVGQRTLMHQVLVCLLAGGNA
                     LLEGVPGLGKTMLVRTLARVLDCQFSRIQFTPDLMPADIIGTNILVEDEHGGRRFQFQ
                     QGPIFANLLLADEINRATPKTQSALLEAMQEGTVSVARTVYTLPKPFFVLATQNPLEM
                     EGTYPLPEAQLDRFLFKLNVTFPTAAELVAISDRTTSGVEPVINHVVDASEIVAMQHL
                     SRQTPIATHVTSYAVRILRATHPDNPEAATMSKRYVRYGGSPRALQAMILAGKIMALL
                     DGRYNVAFRDIRTAALPALRHRLILNFDGEAEGISPDSIIAAVIDETPEEESKR"
     gene            2108..4117
                     /locus_tag="VFH60_10460"
     CDS             2108..4117
                     /locus_tag="VFH60_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6314250.1"
                     /translation="MGLLAPFLALAALLPLGIIVALYLLRLRRPMAPVASLHLWSVLM
                     RDREANTLWQRLKVTPLLLLQVLALLVLTIALARPWVSSPQPVGQNIILVMDVSASMG
                     ATDARERGLETRLQAAQSKARSLVDEVAQGASMTLISSDEHASIVVHSTDDKARLRSA
                     IDGLAVRAAGTDMAEAVELAAAVAARQGNSVIWIMSDGAFPATIEQAGEVQAQVRFFP
                     VGTNSRNQGITALSLQQDAGSLSLFVQIMHAAEVTASRRLDLAVDDAPWNARTVSIGP
                     GLSTELIIEDVPLTARVVAAQLALPDSLPADDQAWTINRASVPANVLLVTDGNKFLEL
                     ALSLLPMVTLYKVAPADYDPAAKLEGGAPVDLTVFDSSVTTTTLITLPQSSILMIAPH
                     TSNALLEVSGVMTQPVPLLAPQFEAGNGQPASQDGRDPLLRYVDLSGLHIAEAYQLAV
                     PQWGRVVLASEQGPLVIAGEEGGRKVAALAFDLHDSDLPVQPAFPLLVRNLVTYLLPE
                     PAGGLPILVTPGEAVGIEAVSPQVDRVLVEDPHGKEWVYPVTAGNPRVAFGETSLLGV
                     YYVTQYSGETIVAQEAFTANLFSRDESVITPNYAPQLPFGVNAAANSGGAGGQDSFQR
                     EIWPWIAFAGFVVLLLEWLYAQRMAFRRAATEIRTRRALRRAERA"
     gene            4128..>5023
                     /locus_tag="VFH60_10465"
     CDS             4128..>5023
                     /locus_tag="VFH60_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET6314251.1"
                     /translation="MDLPFSISEPRLLILLVTVVPVVILGVLSARARPRDRGRINAST
                     VIRSLILVLITLALAGLQWVSSGGPLNVVFLIDESASVPQESSDAAREYVRKAIESMG
                     PEDRAGVVLFGERAIVDRALSPDTTWQPVGKHPANLATNIADAIQVGNALFPEGGSRR
                     IILLSDGAETIGKARDLAARAGLTGVQLSVVPLGAQSQNEVAVDRLASPNNVPAGQQF
                     NVRVLLKSTSARAASVSLFDGAEPAGRQDVQLKPGDNVVEFSMTASSEGFHVIRARVE
                     SVDDRYVENNEESSFTIVRKAPT"
BASE COUNT          982 a         1576 c         1659 g          806 t
ORIGIN      
        1 gcttccgggc agccgaccag gagtacgaat cggctgccgc catcgagaac accctggcga
       61 agctggacga ggggcggcag agtatctcgc aggcaggcaa gccacccacc aagcgcgggc
      121 aggtaggttt caggcggcag agcggaggcg atagcgcgaa ctcgaacgaa ggcgcggcaa
      181 ccccaggcgg ccaggataca ggtggtgcgg gcgcggactc cgaaggcgcg gataacacag
      241 ccccgtacaa cagctccaac gtcggtggga atgcggcgtc gttcggggga gggcagccag
      301 gctcgcccgc gaacggagga caaccggggc agcaaccctc gtcgggtggc ggaccccccg
      361 gcggacccaa ctctgacgtg gcgggcggca ccgcgggcgg agggcaggac gacggcagca
      421 agggcactct ccagggggag atcaccggcc cggtaggcgg cgcgggtggc gcgataagcc
      481 gggtgcaaaa tcctgcgggc cagggcgagg gcggctcctc ggcccctgcc tcggaggaca
      541 acccgccgcc cgataacgag caggtctacg tcccctccca ggaaggcgcg ccctcttctg
      601 ctgaagagcc ttcggtagcg gggcaggctg cgccggaacc gcccaacacg gatgggctag
      661 agggcagagt gggagagggc gggggcggcg aggaaaggcg cggccagagc gggccgggca
      721 ccctcgcgcc catacacacc ccttacaagg aagtcatagg gcaatacgcg gagcgcgcta
      781 ccgagtcgct ggacagggca tatattcctc ccgacgcgaa ggactatgtc agagaatact
      841 tcacccaact tggcaaataa ggaagacggc agtgcggcgc acaaatgttg tcactctaat
      901 ctaggagcaa ttagtggcta tcagtcaaca gggcacacag acgggccagc aagcggttgg
      961 taaggagcaa atacccgaaa tcacccctga gcagttcagg gacaaagcga tacgcatcga
     1021 gcatgagata ggcaaggtga tagtgggcca gcgcacgctg atgcaccagg tgcttgtctg
     1081 cctgctagcg ggcgggaacg ccttgctgga aggggtgccc ggcctgggta agacgatgct
     1141 ggtgcgcacc ctggcacgtg tgctcgactg ccagttcagc cgcatacagt tcacgcctga
     1201 cctcatgcct gcggacatca tcggcactaa catcctggtc gaggacgagc acgggggccg
     1261 ccgcttccag ttccagcagg gtcccatatt cgccaacctg ctgctggccg acgaaataaa
     1321 tcgcgccacg cccaagacgc aatcggccct cctggaagcg atgcaggagg gtacggtgtc
     1381 ggtggcgcgc accgtctaca ctctcccaaa gcccttcttc gtgctggcga cccagaaccc
     1441 tctcgaaatg gagggtacgt atccattacc cgaagcgcag cttgaccgtt tcttattcaa
     1501 gctcaacgtc accttcccaa ccgccgccga gcttgtcgcc atatcggacc gcacgacctc
     1561 cggcgtcgag ccggtaataa atcatgtggt ggacgcatcc gaaatcgtgg cgatgcaaca
     1621 cctgtcgcgg cagacgccca tcgccacgca cgttacctcc tacgccgtgc gcatattgcg
     1681 cgccacccac cccgacaacc cggaggccgc gaccatgtcg aagcggtacg tgcgctacgg
     1741 cggctcgccc cgcgccctgc aagcgatgat attggcgggg aagataatgg cgctgctaga
     1801 cggtaggtat aacgtggcct tccgcgacat caggacagct gccctgcccg cgctacgaca
     1861 ccgcctcatc ctcaacttcg acggcgaggc cgaaggcatc tcccccgact ccataatcgc
     1921 cgcggtgatt gacgagacgc cggaggaaga aagtaagcgc taagcgcagg ggtcaggggt
     1981 cagggattag ggatcagggt atattgagta aacatgaccc aatcggccac caccggcaat
     2041 gacagtagcg gcaggttctg atccctgatc cctgatccct ggcccctcat aacaggtaga
     2101 acatcttatg ggattactcg cgcccttcct cgccctggcg gccttgctgc ccctgggcat
     2161 catagtagcc ctgtacctgc tcaggctgcg gcggccaatg gcacctgtgg cgagcctgca
     2221 cctgtggtca gtgctcatgc gcgaccgcga ggcgaacacg ctctggcaga ggctcaaggt
     2281 gacgcccctg ctgctgctgc aagtccttgc cctcctggtg ctcaccatcg ccctggcgcg
     2341 gccctgggtt agctcgcccc agcctgtggg gcagaacatt atcctcgtaa tggatgtgtc
     2401 ggcaagcatg ggcgcgacgg atgcgcggga gcgcggtcta gagactcgcc tacaggcagc
     2461 acaaagcaag gcccgctcgc tggttgacga ggtggcccag ggcgcaagca tgacgctcat
     2521 ctcctcggac gagcacgcgt ccatagtcgt gcattctacc gacgacaagg cccggctgcg
     2581 ttccgccatt gacggtctgg ctgtacgggc cgcaggcacc gacatggccg aggctgtgga
     2641 actggcggcg gcggtcgcgg cacggcaggg caacagcgta atatggataa tgtcggatgg
     2701 cgcgttcccc gctacgattg agcaggccgg ggaggtgcag gcccaggtgc gcttcttccc
     2761 ggtgggcacc aacagccgca accagggcat caccgccctt tcgttgcagc aggacgcggg
     2821 cagcctgagc ctattcgtgc agattatgca cgccgccgag gtgacggcca gcaggcggct
     2881 cgacctggcg gttgacgatg cgccctggaa cgcgcgcacg gttagcatcg gacccggcct
     2941 ctcgacggag ttgataatcg aggacgtgcc cctcaccgcc agggtggtgg ccgcccaact
     3001 ggccttaccc gactctctgc cagcagacga ccaggcgtgg acgattaacc gggcgagcgt
     3061 gcctgccaac gtgctgttgg tcacggacgg caacaaattc ctcgaattgg ccctgtcgct
     3121 gctgccgatg gtcacgctct acaaggtcgc gcccgccgac tacgacccgg cggcgaagct
     3181 ggagggcggc gctcccgttg acctgaccgt gttcgacagc agcgtgacga ccacgactct
     3241 tataaccctg cctcaaagca gcatattgat gatcgcgccg cacaccagta acgccctgct
     3301 ggaagtgtcg ggcgtgatga cgcagcccgt gccgttgctg gccccgcagt ttgaagccgg
     3361 gaacggccag ccagccagcc aggatgggcg agaccctttg ctgaggtacg ttgacctgtc
     3421 ggggctacat atcgccgaag cgtaccagtt ggccgtgccg cagtggggcc gggtggtgct
     3481 ggcgagcgag caggggccgc tggttatcgc gggcgaagag ggcgggcgca aggtggcggc
     3541 gctggccttc gacctgcacg acagcgacct gccggtgcag cccgccttcc ccctgctggt
     3601 gcgcaacctc gtgacttacc tgctgccgga gcctgccggt gggctgccca tcctggtgac
     3661 gcccggcgaa gctgtgggca tcgaagcggt atcgccccag gtagacaggg ttctggttga
     3721 agaccctcac ggcaaggaat gggtctaccc agttacggct ggcaaccccc gcgtggcgtt
     3781 tggtgagacg agccttctgg gcgtctatta tgtgacgcag tacagcgggg agacaatcgt
     3841 agcgcaggag gctttcaccg cgaacctatt ttccagggac gagtcggtga taacgcccaa
     3901 ctacgcgcca caactgccat tcggggtgaa cgcagccgcg aactccggcg gggccggggg
     3961 gcaagacagc ttccagcgcg agatatggcc ctggatagcg ttcgcgggct tcgttgtgct
     4021 attgcttgag tggctgtacg cgcaacggat ggctttccgc cgcgccgcta ccgagatacg
     4081 aaccaggcgc gccctgcgaa gggccgagcg cgcgtgagag atatagcgtg gacttacctt
     4141 ttagcataag cgagcctcgg ctcctcatat tgttggtgac cgtcgtgccg gtcgtcatcc
     4201 tgggcgtact gagcgcgcgc gccaggccgc gcgaccgggg acgcatcaac gcaagcacgg
     4261 tcatccgctc gctcatactc gtgctgatta cccttgccct ggcgggcctg caatgggtgt
     4321 catcgggcgg cccactgaac gtggtcttcc tcatagacga gtcggccagc gtgccccagg
     4381 agagcagcga cgcggcgcgg gaatacgtgc gcaaagctat cgagagcatg ggaccggaag
     4441 accgcgcggg ggtggtgctg tttggcgaga gggccatagt ggaccgcgcg ctgtcgcccg
     4501 acacgacatg gcagcctgtg ggcaagcacc ccgccaacct ggcgaccaac atagccgacg
     4561 ccatccaggt gggcaacgcg ctcttccccg aaggcgggtc gcggcgcatc atcctcctga
     4621 gcgatggtgc cgaaactata ggtaaggcgc gcgacctggc cgcgcgcgca gggctaacgg
     4681 gcgtccaact ctccgtcgtg ccactgggcg cgcaatcgca gaacgaggtg gcggtagaca
     4741 ggctggcgag tcccaacaac gtgcccgcgg gccagcagtt caacgtgcga gtgctgctga
     4801 agagcacgtc cgcccgcgcg gcctccgtga gcctcttcga tggcgctgag cctgcgggcc
     4861 gacaggacgt gcagttgaag ccaggggata acgtggtcga gttcagtatg actgcttcga
     4921 gcgagggctt tcacgtaatc agagcgcgcg tagagtcggt ggacgatagg tacgtcgaga
     4981 ataacgaaga gtcctcattc acaatagtgc gaaaggcccc tac
//
LOCUS       DASRWZ010000664         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1355807, whole genome shotgun sequence.
ACCESSION   DASRWZ010000664 DASRWZ010000000
VERSION     DASRWZ010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355807"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1390
                     /locus_tag="VFH60_10470"
     CDS             293..1390
                     /locus_tag="VFH60_10470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6314252.1"
                     /translation="MEAAPATWYVRPYRPGDESQLAALYAEVFGRERPLDVWRWKLME
                     RGVSPASTWVAATHEGERIVGHYGGIPLRMRLRGEEQPMVHSVEAMSARDFRRQGILT
                     RLGEAAHEAWGRAGYVAVLGLPNEQWGTRNYAVGFRRVFPLAWLRFPLHVERVVARAG
                     RLPAPLDGPAGVLAGAGSAAWRWAFRSIMHARRGSSDLQVKEADGPADLDRLWSRLAG
                     EYENVVVRDGAWVDWRFVRAATHHHKVLVAQKVGEPTGYIAYYLADTGERANGYIADI
                     FLGRKDLATASALVKSALDDLSARGAGMVLATAPPGSALYSRLRGLGFLPAPRRTAFN
                     FEIVPLHPNVDPASLADPRTWHLTAGDFDVI"
     gene            1387..2316
                     /locus_tag="VFH60_10475"
     CDS             1387..2316
                     /locus_tag="VFH60_10475"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET6314253.1"
                     /translation="MIADMRASLHSILRWLARRAVFPGVERAWELGFLLAGLLLRPAA
                     QRLTPTGSDYVLVVAPHPDDETIGCGGSIARHTEAGDRVRVLVVTDGGGSRAGGIGRD
                     EMRRLREVEADRAVRRLGPVDLVQLGLPEGRWDSEDLLRTLAAQLRDDPPTLIYTTSC
                     VDFHQEHAKVARVLARALRSLGGATRPRVRVYELQVPLTPALANVVIEVGGLAFSRKA
                     GALAEYRTQEGALKSLRRQSKYLRRLYRTHQPVEVFWELDAEGFCRLMETGSRRPPYR
                     STRPRPFTDGLAWLAGLRTRVRLKKLAERRAAS"
     gene            2313..3275
                     /locus_tag="VFH60_10480"
     CDS             2313..3275
                     /locus_tag="VFH60_10480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6314254.1"
                     /translation="MNSTPRVSVIIPTYNRAAMLRETLKSVMSQTVPPAEVVVADDGS
                     TDDTPGVVRKFEGAGPAVIYLRLPHSNRRGPIRNAGVAASTSPLLAFLDSDDLWEPVR
                     LERQLEEWERAPAAGFAFCKIRRFDETGIFGAPWPPPTARPAGHILGDLLEEPLAGGS
                     TLMAKRQAFERVGGFRDLRLNEDYELTLRLAAHYEASYVPEPLVLVREHAGRTTLAGR
                     EAPMLDYLRIVRRFVAANPDLPNGTKARARRGMANVHYKLAGVYLELGDRAAARRHLW
                     AMSRLKPLDRRAPAAWLKTLAPRGSKLPPPGLPRLGRPLGACPA"
BASE COUNT          557 a         1045 c         1127 g          548 t
ORIGIN      
        1 ggaaaggcga tctatgccgc ttctgtgacc gttaccatct cgctcgtgct ctgctgcggc
       61 ctgttgtgtg aagttagtgg tatgcatgat agccccttgt ctgagaaggg tagtgaagcg
      121 ttctttattt cttcagccaa ctgctgaaga ttatagcaga cttggcgctg tagtgcgttt
      181 ggcagcctgc tgtaaccctg cctgtagaaa cagtaggagc ctcgcctgcc ggtgcgctat
      241 actgaaggac caacccggca aatcgcccgc tggagaagaa cccttgcaat cgatggaagc
      301 cgcgcccgcc acatggtacg tgcgccctta caggcccggc gacgagtcgc aactcgccgc
      361 gctgtatgcg gaggtgttcg gccgggagcg ccccctggac gtatggcggt ggaagctgat
      421 ggagcgcggg gtctcaccag cctctacgtg ggtagcggcg acccacgagg gcgagcgcat
      481 agtagggcac tacggtggca ttccactaag aatgcggttg agaggcgagg aacagccgat
      541 ggtacattcg gtggaggcca tgtcggcccg cgacttccgg cggcagggga tacttaccag
      601 gctgggcgaa gcagctcacg aggcatgggg gcgggccggt tacgtcgcgg tgctgggcct
      661 ccccaacgag caatggggca ctcgcaacta tgcggtcggt ttcagacgtg tcttccccct
      721 tgcgtggttg cgtttccctc tgcacgtcga gcgtgttgtg gcgcgtgccg gccgcttgcc
      781 cgcgccgctg gacgggccgg ccggagtact cgcgggggca gggtccgccg cctggcgctg
      841 ggccttccga agcatcatgc acgcgaggag gggaagcagc gatttgcaag tgaaagaggc
      901 cgacggcccc gctgatcttg accggctttg gtcgcgcctg gcgggggaat acgagaacgt
      961 ggtggtgcgc gacggcgcct gggtagattg gcgtttcgtg cgcgccgcca cgcaccacca
     1021 caaggtgctg gtggcccaaa aggttggcga gccgaccggg tacatagcct actacctggc
     1081 agacacgggc gaacgcgcca acggctacat cgcggacatc ttcctggggc gcaaggacct
     1141 cgcgaccgcc tcggccctgg tgaagagcgc cctggacgac ctgtcggcac ggggcgcggg
     1201 catggtgctg gcaaccgccc ctcccggctc ggccctgtac agccgcctga gagggctggg
     1261 cttccttccc gcgcccaggc ggaccgcctt caacttcgag atcgtgcccc ttcaccccaa
     1321 cgtagacccc gccagcctcg cggacccacg cacctggcac ctcaccgccg gtgacttcga
     1381 cgtgatatga ttgctgacat gcgtgcaagc ctccactcta ttttacgatg gctggcgcgg
     1441 agagcggtct tccccggcgt ggagagggcc tgggaactag gcttcctgct ggcggggctg
     1501 ctgctccgtc cggcagcgca acggctcacg cccaccgggt cggattacgt gctggtggtg
     1561 gcaccccacc ccgacgacga aacgattggc tgtggaggct ctatcgctcg ccacaccgaa
     1621 gccggggacc gtgtgcgcgt gctggtcgtc acagatggcg gcggttcgcg cgcggggggc
     1681 atagggcggg acgagatgcg gcggctgcgt gaggtggagg cagaccgcgc ggtgcggcgg
     1741 ctggggccgg tggacctggt gcaattgggc ctccccgaag gccgctggga ctcggaagat
     1801 ttgttgcgaa cgcttgcggc acagctgcga gacgacccgc caacgcttat ttacaccact
     1861 tcgtgcgtcg attttcacca ggagcacgca aaggtggcgc gggtgctggc ccgtgccctc
     1921 cggtcgttag gaggagcgac gcgtcccaga gtgagggtgt acgagctaca ggtgcccctc
     1981 accccggcgc tggctaacgt ggtaatagag gtgggagggc tggcattttc aaggaaagcc
     2041 ggggcgctcg ccgagtacag gacgcaggaa ggcgcgctca aaagcctgcg caggcaaagc
     2101 aagtacctgc gcaggctgta ccgcacccac cagccggtgg aggtcttctg ggaattggat
     2161 gcggagggct tttgcaggct gatggagacc ggcagccgca ggccccccta caggagcacc
     2221 aggccgcgtc ccttcaccga tgggctggcc tggctggcgg gcttgaggac gcgggtgagg
     2281 ctgaagaagc tggccgaacg tcgagccgcc tcatgaacag cacgccccgc gtcagcgtca
     2341 ttatcccgac ctacaaccgt gcggctatgc tgcgcgagac cctcaagagc gtcatgtcgc
     2401 agacggtgcc cccggccgag gtggtggtgg cggacgatgg ctccaccgac gacacgcccg
     2461 gcgtggtgcg caagtttgaa ggcgcaggcc ccgccgtcat ctacctgcgg ctgccacact
     2521 ccaaccggcg cggccctata cgaaacgcgg gagtggcagc cagcacaagc cccctcctgg
     2581 ccttcctcga ctcggacgac ctctgggagc ctgtccggct tgagaggcag ctagaggaat
     2641 gggagcgcgc gcccgcggcg ggtttcgctt tctgcaaaat ccgccgcttc gatgaaacgg
     2701 gcatctttgg tgcgccctgg ccgcccccca ctgcccggcc cgccggccac atcctgggtg
     2761 accttttgga ggagcctctg gcgggcggga gcacactcat ggcgaagcgg caggcgttcg
     2821 aacgggtcgg cggcttccgc gacctgcgcc tgaacgaaga ctacgagctg acgctgaggc
     2881 tcgccgccca ttacgaagcg agctacgtgc ccgaaccgct ggtgctggtg cgcgagcacg
     2941 cgggccgcac cacgctggcc ggacgcgaag cgcccatgct cgactacctg cgcatagtcc
     3001 ggcggttcgt ggccgccaat cccgacctgc ccaatggaac taaagcccgc gccaggagag
     3061 gcatggcaaa cgtccactac aaactggcag gcgtctacct tgagctgggc gacagggcgg
     3121 cggccaggag gcacctgtgg gccatgtcca ggctgaagcc gctggaccgc cgcgcccctg
     3181 ctgcctggct caagacgctg gcaccacgcg ggagcaagtt gccccctcct ggccttcctc
     3241 gactcggacg acctctggga gcctgtccgg cttgaga
//
LOCUS       DASRWZ010000665         5038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_54115,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000665 DASRWZ010000000
VERSION     DASRWZ010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54115"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1024)
                     /locus_tag="VFH60_10485"
     CDS             complement(113..1024)
                     /locus_tag="VFH60_10485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6314255.1"
                     /translation="MKIGLQVNNFTWDGGEEQLGETFGAIGRRAEEAGFDSFWVMDHF
                     FQIGGIGPAEWPMLEGYSALAFIAGQTSKMKLGTMVTGVTYRHPGILVKTVTTLDVLS
                     RGRAYLGIGAAWFEREHLGLGVPYPPLKERFERLEETLQIAHQMWSGEVGAYNGTHYQ
                     LAETLNVPQVISKPHPPILIGGTGEQKTLRFVAKYGDACNLFVRMGEDVLKHKLEVLR
                     GHCETEGRPYEEIEKTSLDTLLVTRDGQGDMAMSPQQAIDYFGNLAQLGFDHALISMR
                     NVSHPDAFDVFRDEVVPAVHEITPAGR"
     gene            1239..2279
                     /locus_tag="VFH60_10490"
     CDS             1239..2279
                     /locus_tag="VFH60_10490"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET6314256.1"
                     /translation="MSTRSLRLVMSSLLAFLFAFVSSYSALSASARSLSDAHDRHSAA
                     LAPARPYRVALQVGHYKNNELPPSLSSLSGHTGAYGGGRSELELNLDVANRSARLLRA
                     QGVIVDILPATVPTGYTADAFIALHADGNSSPAARGFKISTRWRSEVALQDYMLVDML
                     TDAYRAATALPEDGNVTRNMRGYYAYSPWRPDYRVSNFTPSAIVEMGFVTSAADRAVM
                     FGQTDKVTSGVVTGILAFLKSAYGSSPASRSYGYGLVDADINKPRPTPAPRTGTQGPQ
                     SRQSQGDWQLLLMGKSMINVYSRAGGGSVLAQLPKGKFHHSIFRDGDYYRITLPNGQE
                     GWIHRNAIIVQM"
     gene            complement(2335..3222)
                     /locus_tag="VFH60_10495"
     CDS             complement(2335..3222)
                     /locus_tag="VFH60_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6314257.1"
                     /translation="MRERLRHQEEVLALLQRAFGIEEWTFTLPPGTGTETYFAHSGKR
                     SCFIKLGAYPDRTVAMSEAGLSPPVLAIERLEDGTSVLIQAYVKGRTPRRSDYHEHLE
                     QVATLLRKMHHDPNVQKALHAPRSDLYSDVGRDVVADVRRRWEAYKAALPDVAAFVDS
                     SLDYMAQQVEDFTGSGLVASHNDFCNANLLLTGDGRIFVVDLEAMSMEDPARDVGVAL
                     WWYYPPEMRSRFLQIAGYGDGPKFQHRMRVRTALHCLVITLPRAGSFDRLDPSSYGER
                     LADFRAALAGEENPEGYGD"
     gene            complement(3256..>5038)
                     /locus_tag="VFH60_10500"
     CDS             complement(3256..>5038)
                     /locus_tag="VFH60_10500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET6314258.1"
                     /translation="RNVVVRRSSDGQLSDVTPPPFNARTRVHEYGGGAFFVSNGTLYF
                     SNYADQRLYRQEPGGQPEAITPEVDMRYADGVLDHRRGRIICVREDHTGEGEAVNTLV
                     GISLEGGDSGTVLLSGNDFYSTPRISPDGTCLAWLTWNHPNMPWDGTELWVGEFAEDG
                     SIRDRKLVAGGQEESIFQPEWSPDGTLYFVAEYTGWWNIYRLRGGQVENVCEMEAEFG
                     VPQWQFGATSYGFESAQTIICRYIEGGATKLGWLDTRKGLLVPIETPYTYIWHLKVKK
                     GQAIFIGGSPTQSASVVRLDLATLRIEVLHKSSTLTIDPAYLSVPEHIEFPTENGQTA
                     HAVYYPPTNPGHTPDCEELPPLLVISHGGPTSSTSTVLAPNTQFWTSRGFGVLDVNYG
                     GSTGYGRAYRQRLNGQWGVVDLNDCVNGALYMVKQGRADKNRLAIRGGSAGGYTTLCA
                     LTFSDVFSAGASYYGIADLSIFVHDTHKFESRYLERLVGPYPEKEELYRQRSALHHIE
                     RLSAPVIFFQGLEDKVVPPNQAEIMVAALREKGVPVAYLPYEGEQHGFRRAENIKRSL
                     DAELYFYSRIFGFEIADEIEPVEIENL"
BASE COUNT          911 a         1725 c         1401 g         1001 t
ORIGIN      
        1 ccgccaggac gaggcatgcc tcgcccccta cccggaatcc gaacgctcac ataccagggc
       61 agaagaccag gcatctcccc cggccctctc ccctcattcc tcgaacctct gtttaccgtc
      121 cggctggggt aatctcatgc accgcaggca ccacctcgtc gcggaagaca tcaaacgcgt
      181 ctgggtggct gacattccgc atgcttatca gggcgtggtc gaagcctagc tgggccaggt
      241 tgccgaagta gtctatcgcc tgctgcggcg acatcgccat atcgccctgg ccgtcccgcg
      301 tcacgaggag ggtatcgagg ctcgtcttct ctatctcctc atacgggcga ccctcggtct
      361 cgcaatgccc acgcagcact tccagcttat gcttcagcac gtcttcgccc atgcggacga
      421 agagattaca cgcatcaccg tacttcgcca cgaagcgcag cgtcttctgc tcccccgtgc
      481 cgccgatgag gattggcggg tgcggcttgc tgatgacctg gggcacgttc agcgtctccg
      541 ccaattggta gtgtgtgccg ttgtacgccc cgacctcgcc cgaccacatc tggtgggcta
      601 tctgcaaagt ctcttccagc cgctcgaacc gctctttgag tggcgggtag ggcacgccca
      661 ggcccaggtg ctcgcgctcg aaccacgccg cccctatgcc caggtaggcg cgcccgcgtg
      721 agagcacgtc cagagtcgtc accgtcttga ccaggatgcc ggggtggcgg tacgtgaccc
      781 ccgttaccat cgtccccagc ttcatcttcg acgtttgccc cgcgatgaac gccagcgcgc
      841 tataaccctc aagcatgggc cattccgcag ggccgatacc tccgatctgg aagaagtggt
      901 ccatcaccca gaagctgtcg aagcccgcct cttcagcccg gcggcctatc gctccaaacg
      961 tctcccctag ctgctcctcg ccaccgtccc atgtgaaatt atttacctgc aatccgatct
     1021 tcatctctta tatctcctct gccgatatgg tccttcaata gtttcgctat atcccctggt
     1081 cgctcaccct gcgtgctttt cgcggggtcc agcgccagta taacacatgc ccgcccttgc
     1141 attccctggc ttatgccatg tataatcaag gcaggtagtg cccttacccc ggttgctctg
     1201 aaccctctcc ggtccctccc gcccatcatg gagctattat gtccacgcgc tctcttcgcc
     1261 ttgtcatgtc atccctcctg gcgttcctct tcgcattcgt ctcgtcttat tctgcgttgt
     1321 ccgcgagcgc ccgctccctc agcgacgccc acgaccggca cagcgccgcg ctcgcccctg
     1381 ctcgccccta cagggtcgcc ctacaggtag gccactacaa gaacaacgaa ctgcctccct
     1441 ccctttccag cctctccggg cataccggcg cttacggcgg agggcgctca gagcttgagt
     1501 tgaacctgga cgtggcgaac aggagcgcgc ggctcttgcg tgcccagggg gtgatagtgg
     1561 acatcctccc cgccaccgtg cccaccggct acacggcgga cgctttcatt gccctccacg
     1621 ccgacggtaa ctcttctcca gcagcacgcg gcttcaagat cagcactcgg tggcgcagcg
     1681 aggtagcctt gcaggattac atgctggtgg atatgctcac cgatgcctac cgcgccgcca
     1741 ccgccctgcc cgaagacggc aacgtcacgc gcaacatgcg tggctactac gcctactcac
     1801 cctggcggcc cgactaccgc gtgtccaact tcaccccctc cgctatcgtg gagatgggct
     1861 tcgtgaccag cgccgccgac cgcgccgtga tgttcggcca gaccgacaag gtgacctccg
     1921 gcgtcgtcac cggcatcctg gccttcctaa agtcggcata cggctcatca cccgcttctc
     1981 gctcctacgg ctacggcctg gtggatgccg acataaacaa acctcgtcct acgcccgctc
     2041 ctcgcaccgg cacgcagggt ccccagtcgc gccaatccca gggcgactgg caattgctcc
     2101 ttatgggcaa atccatgatc aacgtctact caagagcggg cggtggctcg gtgctggccc
     2161 aactccccaa gggcaaattc caccactcca tcttccgcga cggcgactac taccgcatca
     2221 ccctccccaa cggccaggaa ggctggatac atcggaacgc gataatagtc cagatgtaag
     2281 cacgaggggc caggggtcag tgcgttcagt gcgtgctgta gtaccggcta ttgtttaatc
     2341 cccatacccc tcggggttct cctcgcctgc cagggctgcg cggaaatccg ccaggcgttc
     2401 gccataggaa gaaggatcga gcctgtcgaa gctgcccgcc ctggggaggg ttatgacgag
     2461 gcagtgcagc gctgtacgca cgcgcatccg gtgctggaat ttggggccat ctccataccc
     2521 ggcgatttgg aggaaccggc ttcgcatctc aggcggatag taccaccaga gcgccacccc
     2581 aacgtcgcgg gccgggtcct ccattgacat cgcttccagg tcaaccacaa agatgcggcc
     2641 atcgccggtt agcagcaggt tcgcgttgca aaagtcgtta tgcgaggcga caagccctga
     2701 ccctgtgaaa tcctcaactt gctgtgccat gtagtcaagg ctactatcta cgaacgcggc
     2761 gacatcgggg agtgcagcct tgtaggcctc ccaacggcgg cgaacgtcag ctaccacgtc
     2821 gcgtcccacg tcgctataca ggtcggaccg aggcgcatgc agtgccttct gcacgttggg
     2881 gtcatggtgc atcttgcgaa ggagagtggc gacctgctct agatgctcgt ggtagtcact
     2941 ccggcgtggc gtgcggccct tcacatatgc ctgtatgagc acagaggtgc cgtcttccag
     3001 tcgctctatc gccagcacgg gcggggagag tccggcttcc gacatggcta cggtcctgtc
     3061 cgggtaagcc cccagcttga tgaaacaact gcgcttcccg ctatgcgcga aatacgtctc
     3121 ggtccccgtg cccggcggaa gagtaaaggt ccactcttcg atgccgaaag ccctctgaag
     3181 gagagccagc acttcctcct ggtgccgaag tctctcgcgc ataaccccta acccctaact
     3241 cctaacccct gaccactaca aattctctat ctccaccggc tctatctcgt ccgctatctc
     3301 gaagccgaaa atgcgcgagt agaagtatag ctcggcgtcc aggctgcgct tgatattctc
     3361 ggcgcgccgg aagccgtgct gctctccctc atacggcagg taggccaccg gcacgccctt
     3421 ctcgcgcaaa gccgccacca taatctcagc ctggttcggc ggcacgacct tatcctcaag
     3481 gccctggaag aatatcaccg gcgcggagag gcgttcaata tggtggaggg cggaccgctg
     3541 ccggtaaagc tcctctttct cagggtaagg ccccaccagg cgttccaggt agcgcgactc
     3601 gaacttgtgt gtgtcatgca cgaatatgct caggtcggct atgccatagt agctcgcgcc
     3661 cgcgctgaat acgtcgctga acgtgagggc gcacagcgtc gtataccccc ccgcgctgcc
     3721 cccgcgtata gccagccgat tcttatccgc cctcccctgc ttcaccatat agagcgcgcc
     3781 gttcacgcag tcgttcaggt ccactacgcc ccactggccg ttgagcctct gcctgtaggc
     3841 ccgcccgtag cccgtgctgc cgccgtagtt tacgtccagc acgccgaaac cccggctcgt
     3901 ccagaactgg gtattcgggg ctagcacggt gctcgtagac gacgttggcc caccatgact
     3961 gatgaccagc agcgggggca actcctcaca gtcaggcgtg tggccggggt tcgtgggcgg
     4021 gtagtagacg gcgtgcgcgg tctgcccgtt ctcggttggg aactcgatat gctccggcac
     4081 agaaagataa gccgggtcaa tcgtgagcgt gctagactta tgcaacactt caattcgcaa
     4141 tgtcgccagg tccagccgga ccaccgatgc agattgggtg ggcgagccgc ctatgaagat
     4201 ggcctgccct ttctttacct tcaagtgcca gatgtaggtg tagggcgtct ctataggcac
     4261 aagcaagccc ttgcgggtat cgagccatcc cagtttagtg gccccaccct cgatgtagcg
     4321 gcagatgatg gtttgcgccg actcgaagcc gtagctggtc gcaccgaatt gccactgcgg
     4381 caccccgaac tcggcctcca tctcgcacac gttctcaacc tgcccgccgc gcaggcggta
     4441 gatgttccac cagccggtgt actcggccac gaagtagagc gtgccgtccg gcgaccactc
     4501 aggctggaag atagactcct cctgcccgcc cgcgaccagc ttgcggtccc ggatcgagcc
     4561 gtcttcagcg aactctccga cccaaagctc ggtgccgtcc cagggcatgt tggggtggtt
     4621 ccaggtgagc cacgccaggc acgtgccgtc cgggctgatg cgcggggtag agtagaaatc
     4681 gttgccagag agtagcacgg tcccgctatc cccaccctca aggctgatgc ccacgagcgt
     4741 gttgaccgct tccccctcgc ccgtatggtc ctcgcgcacg caaatgatgc gccctcgcct
     4801 atggtccagt acgccgtcgg cgtagcgcat atccacctcc ggggtgatgg cttcgggctg
     4861 gccgcccggt tcctggcgat agaggcgctg gtcggcgtag ttggagaagt acagggtgcc
     4921 gttcgataca aagaacgcgc ccccgccata ttcgtgaact cgagtgcgag cgttgaaggg
     4981 cggcggcgtc acatcgctta actgcccatc agagctgcgg cgcaccacga cgttgcgg
//
LOCUS       DASRWZ010000666         3318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_739991, whole genome shotgun sequence.
ACCESSION   DASRWZ010000666 DASRWZ010000000
VERSION     DASRWZ010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739991"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..439
                     /locus_tag="VFH60_10505"
     CDS             182..439
                     /locus_tag="VFH60_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314259.1"
                     /translation="MSNNLGLGEVKQCAHCDGNGVCKAGFGNMRSCKACLVKAGLDQN
                     AMDVITCATCGGKGSVWVGPEVVQIVGSAGQGNPGSGKRLG"
     gene            460..1266
                     /locus_tag="VFH60_10510"
     CDS             460..1266
                     /locus_tag="VFH60_10510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6314260.1"
                     /translation="MEGSAGAEDRSLVEESRHIWDRNAEVWDSVIAAGRGWQTVFIGP
                     VVERLLALRPGETALDIACGNGIFSRRMAELGAYVVAADFSPRLVELARSRTTEHADR
                     VEYHVADATDEAQLVALGEGRFDAAVCSMALMDMPAIEPLYRAVSRMLKPGGRFVFSV
                     SHPCFNTGYTTRMVEEDNSTGEVTHSIKVANYLSVRTSKGVALREQPAEQYYWDRPIG
                     ALLGAAFAAGLVLDALEEPPFKRETAPTDRLDWANYDMPPVLFARLRLPG"
     gene            1397..2086
                     /locus_tag="VFH60_10515"
     CDS             1397..2086
                     /locus_tag="VFH60_10515"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6314261.1"
                     /translation="MNTLYLVRHGENRANITKEFSHTKVDYPLTEKGVLQARQTAEYF
                     KDRHVDEIYSSPLKRAFQTAEMIAATKGLPVTVIESLREVNSGSLEDTPPTEETWRIY
                     LDTVQGWNEGRPEVALPGGENYYMLVDRIKQAIGEIVGDKDGKHIVVAGHGGIFTAAI
                     GWLCRNVQADELRSAHYHNCAISQIEVERTPEGRLEGMLIGWAYCEHLSGPAADLVSP
                     IPDTPVPAERP"
     gene            2083..2214
                     /locus_tag="VFH60_10520"
     CDS             2083..2214
                     /locus_tag="VFH60_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314262.1"
                     /translation="MSEEEGFIGTGEGPRLYYNVVGGGPDVVVMPGSSNDTPPATQL"
     gene            2211..3008
                     /locus_tag="VFH60_10525"
     CDS             2211..3008
                     /locus_tag="VFH60_10525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6314263.1"
                     /translation="MTAPIPKPGHLSGEYGKQFSDRSVVEAYRFRPPYPDELFDVLIS
                     LIGGSPGHVLDAGCGTGPIARRLAGRVDRVDAVDVSEAMIEEGKRLPGGDHPGLRWTI
                     GGMEDAPIEPPYALIVAGQSLHWMEWDVVMPRFARSLAPGAYLAIVNLTVASTPWRED
                     LLRAIQRHSTNREFQPYNLVDELEMRHLFRKVGEKDTAWEPFTQPVEEYIEAIHSMNG
                     FSRRRMSPHEADTFDEEARLIVSPFAIEGKVELQTAANIVWGFPHPA"
     gene            3014..>3318
                     /locus_tag="VFH60_10530"
     CDS             3014..>3318
                     /locus_tag="VFH60_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET6314264.1"
                     /translation="MDPLPLDTILRGDNLELLRQLPDRCVDLIFADPPYNLQLQNELR
                     RPNTTLVDAVDDAWDHFSDFAAYDAYTEQWLAECRRVLKDTGAIWVIGTYHNIFRVG"
BASE COUNT          661 a         1011 c         1103 g          543 t
ORIGIN      
        1 gcgggagtct ggtcctctga ggccaactgt cacattcacg caccagccgt agtaagccgc
       61 aacactcgtc actcgtcact cgtcactcgt cactcgccac gcgtgcccta tgttataatc
      121 tgccttgtca cgctagcggc gggcgttctt tttgtcgggt gaagtgagag gtaggattgt
      181 catgtcaaat aacctggggt tgggcgaggt gaagcagtgc gcccactgcg acggcaacgg
      241 ggtgtgcaag gccggcttcg gcaacatgcg ctcgtgtaag gcgtgcctgg tgaaggcggg
      301 tctcgaccag aacgcgatgg acgtgattac ctgcgctacc tgcgggggca aaggctcggt
      361 gtgggtaggg ccggaagtgg tgcagattgt cggcagcgcg ggccagggca accccggcag
      421 cggcaagcgg ctcgggtagg cgggaggaga gggcagcgca tggagggtag cgcgggggcc
      481 gaggaccgca gcctggtcga agagtcgcgc cacatctggg accgcaacgc cgaggtgtgg
      541 gacagcgtca tagcggcggg caggggctgg caaacggtgt tcatcgggcc ggtggtcgag
      601 cgcctgcttg cgctgcgccc cggcgagacg gcgctcgaca tcgcctgcgg caacggcatc
      661 ttctcgcggc gcatggccga gctgggcgcg tacgtggtcg ccgccgactt cagccccagg
      721 ctggtggagc ttgcccggtc gcgcactacc gagcacgccg accgggtaga gtaccacgtt
      781 gccgatgcta ccgacgaggc gcaactcgtg gcgttgggag aggggcgctt cgacgcggct
      841 gtgtgcagca tggccctcat ggacatgccc gctattgagc cgctgtaccg ggccgtcagc
      901 cgcatgctca agccgggcgg gcgcttcgtc ttctcggtca gccacccttg cttcaacaca
      961 ggctacacga ccaggatggt ggaggaagac aactcgacag gggaggtcac gcactcgatc
     1021 aaggtagcaa actacctcag tgtgcgcacc agcaagggcg tggccctccg cgagcaaccc
     1081 gccgagcagt actactggga ccggcctata ggcgccttgc tgggtgcggc cttcgcggcg
     1141 ggcctggtgc tcgacgccct ggaggagccg cccttcaagc gagagacggc cccaaccgac
     1201 cgtctcgact gggccaacta cgacatgccg ccggtgctct tcgccaggct gcggctgccg
     1261 gggtgatgcc ggggtgatgc cggggtgagt ctcttctgga acggttattg caggtaagtt
     1321 agtctgaggg cgcgcattgg gaatagactg tgcagaccac ggcgtggtac gctggccgtg
     1381 gagtaagcta ggggtaatga acacactgta cctggtcagg cacggagaga accgcgccaa
     1441 catcacaaag gaattctccc atacgaaggt agactacccg ctcaccgaga agggcgtgct
     1501 acaggcccgg cagaccgcgg agtacttcaa ggaccgccac gtagacgaga tctattcctc
     1561 gcccctcaag cgagcgttcc agacggccga gatgatcgcc gccaccaagg ggctgcccgt
     1621 gaccgtaatt gagagcctgc gcgaggtgaa ttcgggcagc ctggaagaca cgcccccgac
     1681 cgaggagacc tggcgtatct acctcgacac ggtgcagggt tggaacgaag gacggcccga
     1741 agtggcccta cccggcggcg agaactatta catgctcgtg gaccggataa agcaggccat
     1801 aggcgagata gtgggcgaca aagacggcaa gcatatcgtc gtggcggggc acggggggat
     1861 attcacggcg gccatcggct ggctctgcag gaacgtgcag gccgacgagc tgcgcagcgc
     1921 ccactaccac aactgcgcca tcagccagat agaggtggag cgcaccccgg aagggcggct
     1981 ggaaggcatg ctcataggct gggcgtactg cgagcacctg tccggcccgg cggctgacct
     2041 tgtatcgccc atccccgaca cgcccgtacc ggcggagagg ccgtgagcga agaagagggc
     2101 ttcatcggaa cgggagaagg gccgcgcctc tactacaatg tggtcggggg tgggccggac
     2161 gtcgtagtca tgcccggcag cagtaacgac acgccgccgg cgacccaact atgaccgcac
     2221 ctatccccaa gccgggccat cttagcggcg agtatgggaa gcagttcagc gaccggagtg
     2281 tggtcgaagc gtaccgtttc cgcccgccct accctgacga gctattcgac gtgctcatca
     2341 gcctcatcgg aggcagccct gggcacgtgc tggatgcagg ttgcggcaca ggccccatag
     2401 ctcgccgcct cgcaggcagg gtggacaggg ttgatgcggt agacgtctcc gaggccatga
     2461 ttgaggaggg taagcgcctg ccgggcggag accaccctgg tctgaggtgg accataggcg
     2521 gtatggaaga cgcacctata gagccaccct atgctctcat agtggccgga cagagtctgc
     2581 actggatgga gtgggacgta gtgatgcccc gttttgcgcg aagccttgca cccggcgcgt
     2641 acctggcaat cgtcaacctg acggtcgcaa gcacaccctg gcgcgaggac ctgctacgag
     2701 cgatacagcg ccactccacc aacagagagt tccagcccta taaccttgta gacgaattgg
     2761 agatgcggca cctgttccgg aaggtgggcg agaaagatac agcatgggaa cctttcaccc
     2821 agccggttga ggagtacata gaggcgatac atagcatgaa tggcttctcg cgccggcgca
     2881 tgtcgcccca tgaggctgac accttcgatg aggaagcccg gctcatcgtc agtccgttcg
     2941 cgatagaggg caaggtcgag ctacagaccg ctgcgaacat cgtatggggt tttccccacc
     3001 ccgcataaac agtatggacc cgctacccct cgacaccatc ctccggggcg acaacctgga
     3061 actgctgcga caactccccg accgttgcgt ggacctcatt ttcgcggacc caccctacaa
     3121 ccttcaactc caaaacgagc tgcgccgccc caacaccacg ctggtggatg ccgtggacga
     3181 cgcctgggac cacttctccg acttcgccgc ctatgacgcg tataccgagc agtggctggc
     3241 cgagtgccgc cgcgtgctga aggacaccgg ggcgatatgg gtgataggca cctaccacaa
     3301 catcttccgc gtcggcaa
//
LOCUS       DASRWZ010000667         4204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_123235, whole genome shotgun sequence.
ACCESSION   DASRWZ010000667 DASRWZ010000000
VERSION     DASRWZ010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123235"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2388
                     /locus_tag="VFH60_10535"
     CDS             <1..2388
                     /locus_tag="VFH60_10535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET6314265.1"
                     /translation="ARLTHAGNTVEGRIEQDEEREIAVISFEEAIPVGEVSLEIAFDG
                     EVSNGLEGLYLSKDGPEELLCTQCEETAARDIFPCFDEPTFKAQFAWKVTTSPDATVL
                     ANGTLESVRESDDGQSKTWTFAPTKPMSSYLVALVIGDVASTQEEEVNGTPIRVYAMR
                     GKEFMGQYAHDFTRRLLPWFEDYFGVPYHFDKYDQVGVPGFAAGAMENSGLVLFRQSM
                     LLMNPQTASWNAEKAVAHVVAHEFAHMWFGNLVTMKWWDDLWLNESFAEWISYKAVNA
                     LSPEYDIWDDSQGGRVRAMSLDALESTHPIYNKVETPSQAAEMFDVITYEKGCAVMRM
                     LESFLGEEAFRAGIRTYMREFSEANAAGSDLWRHLERAAGRPVTRIMESWVTQGGYPI
                     IGVSLEGNTLRLTQKRFFSSPTAPADNAQTWDAPLIIRYEDGAGVHELRHLLSGREGT
                     LQLDVQGEVRWVYANADEIGFYRQDPDDTLLRGLLDNLDKLSPLEQMGLLSDQWALVR
                     NGTNPITRFLEVEQAMLGANNHNVLGMAVGYLFNIEDLLKESGEEQAMERFRSWVGSS
                     FKERLAGLTFEPKEGESQNDAQARRSVLTAMTMLAQDRESVEQAQQWASKEAADPASV
                     DANLAGVFVSTAAQFGDAAHFEKHLATYVQRQKNASSPQERERYLYSLPFFRPQELVE
                     RIFTLIDERTLPTEAVIPLLRDMLGRRHTQLTAWNYIQGHWEFIATLGSLSVPSVITA
                     TGSLPISQRDELEQFYEQHLKGVADQSYARAIETMEQLAEFRARTKGDVVAWFKK"
     gene            2641..3561
                     /locus_tag="VFH60_10540"
     CDS             2641..3561
                     /locus_tag="VFH60_10540"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET6314266.1"
                     /translation="MEPSPILQTDLPLPLFGRGKVRDTYDLGEELLIVATDRISAFDS
                     VLPTGIPYKGQVLTALSAFWFRRTADIVPNHMIAWERRDFPFEWQSWPQEQQAQLLGR
                     AMLVKKAERIDIECVARGYLAGSAWSEYDQTGAITGITLPHGLVPSQQLPEPIFTPAT
                     KAASGHDENVTFERVVELVGQDLAEQLREATLKIYTALAEHARQRGVVVADTKLEFGL
                     LDGQLILIDELGTPDSSRFWEVTKYRFGEDQASLDKQYVRDWLLQSGWDREPPAPALP
                     DEVARQTSEKYREAYRQIVGHDLLNDLKHQ"
     gene            3582..3839
                     /gene="purS"
                     /locus_tag="VFH60_10545"
     CDS             3582..3839
                     /gene="purS"
                     /locus_tag="VFH60_10545"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HET6314267.1"
                     /translation="MQKWLANVYVTLNPVVNDPPGLSIRDALHNLGYEQVQTVRSGKY
                     IRLHMEAPDETSARTAVDAMCQRLLANPVIEDYTFNLERSE"
     gene            3880..>4204
                     /locus_tag="VFH60_10550"
     CDS             3880..>4204
                     /locus_tag="VFH60_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314268.1"
                     /translation="MRDRISRAMIALVVLAGLSLAVPSRAYACTPLALPDLQTARNNA
                     DAIFVGKVVAIDSSPFAAIGARIFDTGHQRVLFNVTSFWKGPETSQAIVSVANKVCSA
                     TIHDLY"
BASE COUNT          808 a         1363 c         1314 g          719 t
ORIGIN      
        1 gcccggctca cgcatgcggg caacacggtc gagggccgca tcgagcagga cgaggagcgg
       61 gagattgccg tcatcagctt tgaggaggcc atcccggtgg gcgaggtcag cctggagatc
      121 gcgttcgacg gcgaagtgag caacggcctc gaggggctgt acctctccaa ggacggcccg
      181 gaggaactgc tctgcaccca gtgcgaggag accgccgccc gcgacatctt cccctgcttc
      241 gatgagccga ccttcaaggc gcagtttgcc tggaaagtca ccacgtcgcc cgacgctacc
      301 gtgctcgcca acggcacgct ggagtcggtg cgggagagcg acgacgggca gtcgaagacc
      361 tggaccttcg cgcccaccaa gccgatgtcg agctacctgg tggcgctggt gataggcgac
      421 gtggcctcta cccaggagga ggaggtcaac ggcaccccca tccgcgtgta cgccatgcgc
      481 ggcaaggagt tcatgggcca gtacgcccac gatttcaccc ggcgcttgct cccctggttc
      541 gaggactact tcggcgtgcc ctaccacttc gacaagtacg accaggtggg cgtgccgggc
      601 ttcgcggcgg gcgcgatgga gaacagcggc ctcgtgctct tccgccagtc gatgctgctg
      661 atgaacccgc agaccgcctc ctggaacgcc gagaaggccg tggctcacgt ggtggcccac
      721 gagttcgccc acatgtggtt cggcaacctc gtcacgatga agtggtggga cgacctgtgg
      781 ctcaacgagt cgttcgccga gtggatttcc tacaaagccg tgaacgccct ctcccccgag
      841 tacgacatct gggacgactc gcagggcggc cgcgtgcgcg ccatgagcct cgacgcgctg
      901 gagagcaccc acccgatcta caacaaggtc gaaacgccct cccaggccgc cgagatgttc
      961 gacgtgatca cgtacgagaa gggctgcgcg gtcatgcgca tgctcgaaag cttcctgggt
     1021 gaagaggcgt tcagggcggg catccgcacc tacatgcgcg agttctccga ggccaacgcg
     1081 gcaggctccg acctgtggcg gcacctggag agggcggcgg gccgccccgt cacgcgcatc
     1141 atggagagct gggttacgca gggcggttac ccgatcatcg gcgtgtcgct ggagggcaac
     1201 accctgcgcc tgacccagaa gcgattcttc tccagcccca ccgcccccgc cgacaacgcc
     1261 cagacctggg acgcgcctct aatcatacgt tacgaggacg gcgcgggcgt gcacgagctg
     1321 cgccacctgc tctccgggcg ggaaggcacc ttgcagttgg acgtgcaggg cgaggtcagg
     1381 tgggtgtacg ccaacgccga cgaaatcggc ttctaccgcc aggaccccga cgatacgctg
     1441 ctcagggggc tgctggacaa cctcgacaaa ctgtcgccgt tggagcagat ggggctgctc
     1501 agcgaccagt gggcgctcgt gcgcaacggc accaacccca tcacccgctt cctggaagtg
     1561 gagcaggcga tgctgggcgc gaacaaccac aacgtgcttg gcatggctgt cggctacctg
     1621 ttcaatatag aggacctgct gaaagagtcg ggcgaggagc aggctatgga gaggttccgc
     1681 tcgtgggtcg gctcctcgtt caaggagagg ctggcggggc ttaccttcga gccgaaggag
     1741 ggggagtcgc agaacgacgc ccaggcccgg cgctcggtgc tcaccgcgat gaccatgctg
     1801 gcgcaggacc gcgagtcggt ggagcaggcg cagcagtggg cgagcaagga agccgccgac
     1861 cccgcctcgg tggacgccaa cctggccggg gtgttcgtct cgaccgccgc ccagtttggc
     1921 gatgcggccc acttcgagaa gcacctcgcg acctacgtgc agcgccagaa gaacgcctcg
     1981 tccccccagg agcgagagcg gtacctctat agcttgccct tcttccgccc ccaggagctt
     2041 gtggagcgca tctttaccct gatagacgag cgcactctgc ccaccgaggc ggtcatcccg
     2101 ctgctgcgcg acatgctggg ccgccgccac actcagctta cggcttggaa ctacatccag
     2161 gggcactggg agtttatcgc gacccttggc agcctctccg tgcccagcgt aatcaccgcc
     2221 acgggcagcc tccccatctc ccaacgcgac gagcttgagc agttctacga gcagcacctc
     2281 aagggagtgg ccgaccagag ctacgcccgc gccatcgaga cgatggagca gctagccgag
     2341 ttccgcgccc gcaccaaggg cgacgtggtg gcctggttca agaagtagcc tcagctagga
     2401 gacggaaccg ccaagacgcc aagagcgcca agagcgcgcc aagattaagt aggtgttcta
     2461 agtgaaaggg ccagggtacg acacatgtac gctggtcctt tcttgtacca cctaagcctt
     2521 tgttctcttt gcgcgtcctt tgcgtctttg cgtctttgcg ttttcgtact cttggcgttc
     2581 ttggcgtctt ggcggttccg tctcttagcg tcgcgcgttt gcacgtggta taatgccgct
     2641 atggaaccca gccctatact tcagaccgat ttgcccctcc cgttatttgg caggggcaag
     2701 gtgcgtgaca cttacgacct cggtgaggaa ctgcttatcg tggcgaccga ccgcatctcg
     2761 gcgttcgact cggtgctgcc caccggcatc ccgtacaagg ggcaggtgct caccgcgctc
     2821 tcggccttct ggttccggcg caccgcggac atagtgccca accacatgat cgcgtgggag
     2881 cggcgcgatt tcccgttcga gtggcagtcg tggccccagg agcagcaggc gcaactcctg
     2941 gggcgggcga tgctcgtgaa gaaggccgag cgcatcgaca tagagtgcgt ggcgaggggc
     3001 tacctggcgg ggtcggcctg gtccgagtac gaccagacgg gcgcgattac cggcatcacc
     3061 ctgccccacg gcctcgtgcc gagccagcag ctaccggagc ctatcttcac ccccgccacc
     3121 aaggccgcat cggggcacga cgagaacgtc accttcgagc gcgtggtgga acttgtcggg
     3181 caggacctgg cggagcagtt gcgcgaggcc acgctgaaga tatacacggc ccttgccgag
     3241 cacgctcgcc agcgcggagt ggtcgtggcc gataccaagc tggagttcgg cctgctcgac
     3301 gggcaactga tcctcatcga cgaactgggc acgcccgact cctcccggtt ctgggaagtg
     3361 accaagtacc gcttcgggga ggaccaggcc agcctcgaca agcagtacgt gcgcgactgg
     3421 ctgctgcaat cgggctggga ccgcgagccg cccgccccag ccctgcccga tgaagtagcc
     3481 cggcagacct ccgagaagta ccgggaagcc tatcgccaga tcgtgggcca cgacctcctc
     3541 aacgacctca aacaccagta gcagggctag agagcatacc gatgcagaag tggcttgcaa
     3601 acgtctacgt gaccctcaat ccggtagtga acgaccctcc cggcctctcg atacgcgacg
     3661 cgctgcacaa cctcggctac gagcaggtgc agaccgtgcg ctcaggcaag tacatccgcc
     3721 tccacatgga ggcccccgac gagacttccg cccgcaccgc cgtagacgcc atgtgccagc
     3781 gcctgctcgc caaccccgtg atcgaggact acactttcaa cctggaacgg tcggaataaa
     3841 aggacagtac gaacaagttg ggtgcggtgt agagacgtta tgagagaccg tatatcacgt
     3901 gccatgatag cacttgtagt gctagccggc ctttcactgg cagtcccctc cagggcatat
     3961 gcctgcacac ctcttgcttt accagactta cagaccgcac ggaacaatgc cgacgcaatc
     4021 ttcgtgggca aggttgtggc catcgatagc agtccctttg cagcgatagg cgctcgcatt
     4081 ttcgatacag gacatcagcg cgtccttttc aatgtcacgt ccttctggaa aggtcctgag
     4141 acctcccagg ccattgttag cgttgcaaat aaggtttgca gcgccacaat acacgactta
     4201 tata
//
LOCUS       DASRWZ010000668         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1013663, whole genome shotgun sequence.
ACCESSION   DASRWZ010000668 DASRWZ010000000
VERSION     DASRWZ010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013663"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="VFH60_10555"
     CDS             complement(<1..1625)
                     /locus_tag="VFH60_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314269.1"
                     /translation="MLPKKNRLVGPAIAATLLFAALVVAGLQAVTSPATAATQPADAS
                     LCWHSFLSPQATAGYETYNGVAGLSANDVWAVGMHTAGNVNGQPRNLTAHWDGFAWRE
                     VPSPGAGQNSDRLNDVAVVAPDDVWAVGEYYTGPGDAEKTGTFIMHWDGKEWSIVGSP
                     SPADRLNSLLSIAAASAKDIWAVGLSGNISEPIQPLILHWDGVAWSQVATPTIPPPPQ
                     AAANSLETGQLLDVSVIRNDDVWAVGSGTGGGALALHWDGKTWSLVDTEAQGALQSVG
                     GVAANDLWALGISQAGAQALHWDGSRWNKVPLPAPSAIITVLHGLAVLGKDDVWVAGY
                     FYPAPNSPAQALVMRWDGKEWVQQERPPIQALGMQLEAAAAVQGNLWVVGHAATGKGF
                     QVVGLLARHTGAPCASPLPTHAVPFYPPAPLPGSNSRTFTETGYTVSGIFLDYWQRNG
                     GLSQQGYPIFAVIGEVSPLNGSLYTVQYFERAVFEYHPENKPPYDVLLSQLGTFQYKK
                     KYPGGAPGQKPNNDPGSVFFPETGTRLGGAFLE"
     gene            complement(1765..2943)
                     /locus_tag="VFH60_10560"
     CDS             complement(1765..2943)
                     /locus_tag="VFH60_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314270.1"
                     /translation="MPITQVSTPRILQHRRFSALSLFLLGLASIILSACDANQPGSSS
                     SEADGCWARVPGPEIEAEFVDVTAVHAISAGDVWAVGHTGTVGGDVSRTLTMHWDGAQ
                     WRTVPSPNGPNPDTGRNSLYAVSASAPDNVWAVGAYTTGGSKFRTLAMRWDGVEWRAV
                     PTPELGEYDDELKAVLAFAPDDVWAVGSYLNHEYEDGHMVVLHWDGVSWTRSPLPLNV
                     THTLLSIAANGPDDIWAVGTQVLHWNGTNWRILPTPTAEGAYFDGVAALGKDDVWVVG
                     NDGTAAITLHWDGQKLNIVHVPGPAADAFLHEATATGGDLWTAGENVDRPSMRETLLM
                     KWTGAQWSLYPNAVPGADARLQGVSAAKDGSLWAAGSVTEGGKSKPLFLRYSAEPCNR
                     "
BASE COUNT          502 a         1000 c          915 g          570 t
ORIGIN      
        1 tactccagga acgccccgcc gagtcgcgtt ccggtctctg ggaagaagac cgagcctggg
       61 tcgttgttcg gcttctgccc cggcgcgccg cctgggtact tcttcttgta ctggaacgtg
      121 cctagctggc tcagtagcac gtcgtaaggg ggtttgttct cagggtggta ctcgaacacc
      181 gcccgctcga agtactgcac cgtgtacaaa ctgccgttga ggggcgacac ttctcctata
      241 acagcgaaga tcgggtagcc ctgctgagac agaccgccgt tacgctgcca gtaatcgagg
      301 aagatgccgc tcaccgtata tccggtctcg gtgaaggtgc ggctattact tcccggcagg
      361 ggagcgggag ggtagaacgg gactgcgtga gtgggaagag gcgaggcaca gggtgcccct
      421 gtatgcctgg cgagcaagcc aaccacctgg aagccctttc cggtggctgc atgtcctacc
      481 acccagaggt tgccctgcac cgccgctgct gcctccagtt gcataccgag cgcctggatg
      541 ggcggcctct cctgctggac ccactccttg ccgtcccagc gcatcaccag cgcctgtgcc
      601 ggggagttcg gggcggggta gaagtagccc gctacccaaa cgtcgtcctt acccagcact
      661 gccagcccat gcaggaccgt aattatcgcg gacggcgcgg gtagcggtac cttgttccac
      721 cgcgacccat cccagtgtag cgcctgcgcc cctgcctggc taatgcccag ggcccaaagg
      781 tcattcgccg ccacaccgcc aaccgattgg agagcgccct gggcttctgt gtccaccagg
      841 ctccaggtct taccgtccca gtgcaacgcc agcgcgccac cgccggtgcc cgagcctacc
      901 gcccaaacgt cgtcgttccg aataacgctc acatctagca gttgccccgt ctccaacgaa
      961 ttggctgcgg cctggggagg aggcggtatc gtaggcgtcg caacctggga ccatgctacc
     1021 ccgtcccagt gcaggatgag aggttgtatc ggttcgctaa tattgcctga caagcctacc
     1081 gcccagatgt ccttagccga agcggccgca atagataaga gtgagttgag cctgtcggca
     1141 ggactggggc tgcccacgat gctccactct ttgccatccc agtgcatgat aaatgtgccg
     1201 gtcttctcgg cgtctcccgg gccggtgtaa tactcaccga cggcccacac atcgtccggg
     1261 gcaacaactg ccacgtcgtt aagcctgtcg ctattctggc ctgcacccgg gctgggtact
     1321 tctcgccagg caaaaccgtc ccagtgggcg gtgagattac gaggctgccc gttcacatta
     1381 ccggcagtat gcatgccgac cgcccaaacg tcattggccg acagccctgc cactccattg
     1441 taagtctcat acccggcggt ggcctgcggc gataaaaagc tgtgccagca caggcttgcg
     1501 tccgcgggtt gggtcgcggc ggttgcgggc gacgtgacag cctgtaggcc cgctaccaca
     1561 agggcggcga aaagtagggt tgcggctata gccggaccca ctagcctgtt cttctttggc
     1621 agcatggctc ttctccgtaa tgtagggttt gcgtttactg tttacctcta cattactcaa
     1681 cgcggctatg cattgcaggt tccgccgcat tccgctggag gcaggccttc cgccggcaag
     1741 ctatgccaac gccccggtaa tcccctagcg gttgcagggt tcggcgctgt agcgcaagaa
     1801 cagaggcttg ctcttgcctc cctccgtcac cgaccccgcc gcccacaggc tgccgtcctt
     1861 tgccgccgat actccctgca agcgagcgtc ggcccccggc accgcgttgg gatagaggga
     1921 ccactgcgcg ccggtccact tcatcagcag cgtctcgcgc atgctgggcc tgtctacgtt
     1981 ctcgcccgcc gtccacaggt cgccgcccgt tgccgtggcc tcgtgcagga aggcatcggc
     2041 ggcggggccg ggtacgtgca cgatgttcag cttctgcccg tcccagtgca acgtgatggc
     2101 agccgtgccg tcgttgccca ccacccagac gtcgtcctta cccagcgccg ccacgccgtc
     2161 gaaataagcg ccctcggctg tgggggtggg gaggatgcgc cagttggtgc cgttccaatg
     2221 cagcacctgc gtgcccaccg cccagatgtc gtcggggccg ttggccgcga tgctgagcag
     2281 cgtgtgggta acgttgaggg gcaggggcga gcgggtccag ctgaccccgt cccagtggag
     2341 cacgaccatg tgcccgtcct cgtactcatg gttgaggtag ctgcccaccg cccacacgtc
     2401 gtcgggcgcg aaggccagca ccgcctttag ctcgtcgtcg tactcgccca actccggcgt
     2461 gggcaccgcg cgccactcta ccccatccca gcgcatcgcc agcgtcctga acttgctgcc
     2521 gcccgtcgtg tacgcgccca ccgcccacac gttatcggga gccgaggccg acaccgcgta
     2581 gaggctgttg cgtccggtgt cggggttagg tccgttgggg ctgggtacgg tgcgccactg
     2641 tgcgccgtcc cagtgcatag taagcgtccg cgacacgtcg ccgccaacgg tgccggtatg
     2701 ccccaccgcc caaacatccc cggcggagat ggcgtgcacg gccgtcacgt caacaaattc
     2761 ggcttcgatc tccggccccg gcacccgcgc ccagcagccg tccgcctcgc tgctggagct
     2821 acccggctgg ttcgcatcgc aggcagacag gatgatgctg gcgagcccga gcagaaagag
     2881 ggagagggcc gagaaacgac ggtgttggag tatcctgggg gtacttacct gggtaattgg
     2941 cacttggtta gctctattct aggcgtggta tagtctcctt cccattg
//
LOCUS       DASRWZ010000669         4014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_260207, whole genome shotgun sequence.
ACCESSION   DASRWZ010000669 DASRWZ010000000
VERSION     DASRWZ010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260207"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..737)
                     /locus_tag="VFH60_10565"
     CDS             complement(162..737)
                     /locus_tag="VFH60_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding-motility protein MglA"
                     /protein_id="HET6314271.1"
                     /translation="MALINVAAREIHCKLVYYGPGLSGKTTNLKYIYSTVPGQARGDL
                     LSIDTETERTLFFDFLPLDLGRVHGYQVRFHIYTVPGQVLYERTRVAVLNGSDGVVFV
                     GDSQRAKLAENVRSLQELARNLTQQGKRFLEFPLVMQYNKRDMPDVLPLQTLEKYLNS
                     MHVPAFEAVASKGVGVFDTLRTISKLVISKL"
     gene            complement(830..1354)
                     /locus_tag="VFH60_10570"
     CDS             complement(830..1354)
                     /locus_tag="VFH60_10570"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET6314272.1"
                     /translation="MVNFAPNNLEPEPRPHTSLYVNEHDSRRIGAQLDRVLLEAGANI
                     TLLLDKSGEVIAARGDKLPTDLTVMGALLAGTFATSREVARMMNDHSFRTLVQQGMRE
                     NTLTELVGESWIVVVMFDKSTHIGLVKDMCRQITPELEAVLLQVQRRSRNMSGLNPSF
                     RTSIEDTIDLLFKD"
     gene            complement(1448..2107)
                     /gene="purN"
                     /locus_tag="VFH60_10575"
     CDS             complement(1448..2107)
                     /gene="purN"
                     /locus_tag="VFH60_10575"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET6314273.1"
                     /translation="MTRGSQVVPGPAARDVPFRLGVLVSGRGSNLQAILDAVARGELQ
                     AEVALVVCNHKDAGAIGRAERARVPVEVHEMRAYASRLAQHQAIATRLEEAGVDLVVC
                     AGWDRVLEPEVVRRFAGRMINVHPSLLPAFGGGLHAIEDALRCGVKITGCTVHFVTEE
                     VDTGPIISQAAVPVLDGDTPETLAGRVHPEEHRLLVEAIKLYSQGKLEVDGRVVKVRN
                     T"
     gene            complement(2094..3158)
                     /gene="purM"
                     /locus_tag="VFH60_10580"
     CDS             complement(2094..3158)
                     /gene="purM"
                     /locus_tag="VFH60_10580"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET6314274.1"
                     /translation="MGAVAANTYRQAGVDIDAGARTVGLMRAAVRGTFGPNVLADLGH
                     FGGLYALDPATEDGPVLVASADGVGTKLKLAFVLGTHRLIGHDIVNHCINDILACGAR
                     PLFFLDYIASGKLVPEQTAEVVTGMAEACRAAGCALLGGETAEMPGMYGAGEYDVAGF
                     IVGMVARDRMLLGHDIEPGDIVLALPSNGLHTNGYSLAREVLGLNGDPERARERLARY
                     DATLGTTLGEALTRPHTSYLDHVAPLLKLAERPIKGMAHITGGGLLDNIPRVLPEGCA
                     VELSTVAWAVPPIFGLIQEAGGIEPREMARVFNMGIGLVLVVSPEHVDLVESRLAGSF
                     RAGRVVAADAGERVTLRDEG"
     gene            complement(3370..>4014)
                     /locus_tag="VFH60_10585"
     CDS             complement(3370..>4014)
                     /locus_tag="VFH60_10585"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6314275.1"
                     /translation="TAERLLAIQPARREAAAQELARFLRELHSFDIETATQAGVVTCG
                     YPFCRTEEGIVSGPAEVHYRRELEKLLAYPQVDPATGAHCGTLVERLLAKNAPGELAH
                     SLVHGDLSQDHVLFDEATNRLSGVIDFTDVVVTTPLLDFVYLHHAYGTDFLTMLLSHY
                     GVADAGATLARVRVLHQWYLAMRLLWVLEHNYQPGIAPRLAALTAARIAEAEPL"
BASE COUNT          710 a         1319 c         1179 g          806 t
ORIGIN      
        1 agttatctct gtccagcgcc ggtatgtagc gcagtgcagt tatctctgtc gtagcgggcc
       61 gatgtagcgc agtgcagtta tctttgtcac ggaaaagccg ccagtgcagc tatatctgtc
      121 cccagtgcag atatctctgt cgccctacag acgcccgaca attacagctt gctaatcacc
      181 agcttgctga tcgtcctcag cgtgtcgaac acgcctacgc ctttggacgc cacggcctcg
      241 aacgccggta cgtgcatcga gttgaggtac ttctccagag tctggagggg cagcacgtcg
      301 ggcatgtcgc gcttgttgta ctgcatgacg agggggaact ccaggaagcg cttgccctgc
      361 tgggtgaggt tgcgtgcaag ctcctgcaac gagcgcacgt tctcggccag cttggcgcgc
      421 tgcgagtcgc ccacgaacac cacgccatcc gagccgttga gcaccgccac gcgggtccgc
      481 tcgtagagca cctggcccgg cacggtgtag atgtggaagc gcacctggta gccgtgcaca
      541 cggcccaggt cgagcggcag gaagtcgaag aaaagggtgc gctcggtctc cgtgtcgatg
      601 gagagtagat cgcccctggc ctgccccggc acggtcgagt agatgtactt caggttcgtg
      661 gtcttgcccg agagacccgg cccgtagtac acgagcttgc aatgtatctc tctggctgca
      721 acatttatca gggccatacg cacctcttgg gattttagat tttagatttt ggattttgga
      781 tttagcgcgg ttctaagcaa atctaaaatc caaaacctaa aatctaaaat cagtctttaa
      841 agagcaagtc tatcgtgtcc tctattgatg tgcggaacga cgggttcagt ccgctcatat
      901 tacggctgcg gcgctggact tgcaacagca cggcctccaa ctccggtgtg atctgacggc
      961 acatgtcctt taccaggccg atgtgcgtgc tcttgtcgaa cattactacc actatccacg
     1021 actcccccac tagctcggtc agggtgttct cccgcatccc ctgctgaacg agcgtccgaa
     1081 agctgtggtc gttcatcatg cgggccactt cccgcgacgt ggcgaacgtg cccgctaata
     1141 aggcccccat gactgtgagg tcggtgggca gcttgtctcc cctggccgct atcacctcgc
     1201 cgctcttgtc gagcagtagc gtgatgttgg cccccgcctc cagcagcacg cggtcgagct
     1261 gcgcaccgat acgcctgctg tcgtgctcgt tcacgtacag gctcgtgtgg ggcctcggct
     1321 ccggttccag gttgttcggc gcgaagttga ccatgttggg taatgagtaa cacgtaacac
     1381 gtaacgcgta atgccttagt cactatagtg ctggtattgt ggcttcttac gtgttacgtg
     1441 ttacgcgtta cgtgttacgt accttcacca ctcttccatc tacctctaac ttcccctggc
     1501 tgtagagctt tatcgcttct accaggaggc ggtgctcttc agggtgcacc ctgccggcga
     1561 gcgtctcagg cgtgtcgccg tcgagcaccg gcacggcggc ctgcgagatt atcggcccgg
     1621 tatcgacctc ctcggttacg aagtggactg tgcagccggt aatctttacc ccgcatcgta
     1681 gcgcgtcctc gatagcatgc agtccgccgc cgaaggccgg tagcagcgac gggtggacgt
     1741 tgatcatcct ccccgcgaac cgcctgacta cctccggctc cagcaccctg tcccacccgg
     1801 cgcacactac cagatcgacg cccgcttctt ccagccgggt cgcgattgcc tgatgttgcg
     1861 cgagcctgga ggcgtaagcc ctcatttcgt gcacttcgac cggcaccctg gccctctcgg
     1921 ccctgccaat cgccccggcg tccttgtggt tgcataccac cagggccacc tcggcctgta
     1981 gctcgccccg cgccaccgca tcgagtatcg cctgcaggtt gctgcccctg ccggagacca
     2041 gcacgcccag cctgaacggt acgtcgcggg ccgccgggcc aggcactacc tggctacccc
     2101 tcgtcacgga gggtgacgcg ctccccggca tcagccgcta cgacacggcc cgccctgaac
     2161 gaccccgcca gcctgctttc gaccagatcg acgtgctccg gcgagacgac cagcaccagc
     2221 ccgatgccca tgttgaacac cctcgccatc tcgcgcggct ctatgccgcc cgcttcctgt
     2281 atcagcccga agatgggagg cacggcccat gccaccgtac tcagctccac cgcgcagcct
     2341 tcgggcagca cgcgcggtat gttgtcgagc agaccgcccc ccgtgatgtg cgccataccc
     2401 ttgataggcc gctccgccag cttgagcaga ggcgctacgt ggtccaggta cgaagtgtgc
     2461 ggccgcgtga gggcctcgcc cagcgtggtg cccagggttg cgtcgtacct ggccagccgc
     2521 tctcgcgccc gctcggggtc gccgttcagg ccgagcacct cgcgggccag cgaatagccg
     2581 ttggtgtgca ggccgttcga gggcagggcc agcacaatat cgcccggctc gatgtcgtgt
     2641 ccgagcagca tgcggtcgcg ggccaccatg cccacgatga agcccgccac gtcgtactcc
     2701 ccggcaccgt acatgcccgg catctcggcc gtctcgcccc ccagcagcgc gcacccggca
     2761 gcgcgacacg cttcagccat gcccgtgact acctcggcgg tctgttccgg cacgagcttg
     2821 cccgaggcta tatagtcgag gaagaagagg ggtcgcgcac cgcacgccag tatgtcgttg
     2881 atgcaatggt tgacgatgtc gtgccctatc agccggtgcg tgcccaggac gaaagcgagc
     2941 ttgagcttgg tgcccacgcc gtcggcgctc gccacgagca cggggccgtc ctcggtcgcg
     3001 gggtcgagcg cgtagaggcc gccgaagtgg ccgaggtccg ccagcacgtt ggggccgaag
     3061 gtacccctga cggccgcgcg cataagcccg acggtccttg cgcccgcgtc tatgtcaact
     3121 cctgcctgcc ggtaggtgtt ggctgctaca gcccccaaac tctcggttcc tcctctgtcg
     3181 ccgttattat acagaagggt gccccgtttt gctatgctat gcacgcgtac tttggtccca
     3241 acaagtgtta ggaccatgta tgtgcttact gcgcaggcaa gcacgccgcc cacctgccgg
     3301 tcctctgcct cacttcttca caatccctcc ccaactttcg tggagggcag aagctacgct
     3361 gagggcttgc tataaaggct cagcctcggc aatacgtgct gcggttaatg ccgcaaggcg
     3421 gggcgctatg ccaggctggt aattgtgctc cagcacccac aacagccgca tcgcaaggta
     3481 ccactggtgc agcacacgga cgcgggccaa agttgccccg gcatccgcca caccatagtg
     3541 ggacaggagc atcgtaagaa agtccgtacc gtaagcgtga tgcaggtaca cgaaatcgag
     3601 cagcggggtg gttaccacga catcggtgaa gtcaatcacg ccgctgagcc ggttggtggc
     3661 ttcatcgaac agcacgtggt cttgagaaag gtccccgtgc accaggctgt gggcaagctc
     3721 acccggcgca tttttagcca gcaaccgttc tactaatgtg ccacagtggg cgcccgtagc
     3781 agggtcaacc tggggatagg ctagcagttt ttccagttcc ctgcggtagt ggacctctgc
     3841 cggacccgac acgatacctt cttcggtccg gcagaagggg tagccgcacg tcaccacgcc
     3901 cgcctgtgta gccgtctcga tgtcgaacga atgtagctcc cgaaggaagc gggcaagctc
     3961 ctgggcggcc gcttctcgcc gcgcaggttg gatggccagc aggcgctcgg ctgt
//
LOCUS       DASRWZ010000670         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_328638, whole genome shotgun sequence.
ACCESSION   DASRWZ010000670 DASRWZ010000000
VERSION     DASRWZ010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328638"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..770)
                     /locus_tag="VFH60_10590"
     CDS             complement(147..770)
                     /locus_tag="VFH60_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314276.1"
                     /translation="MTQESYSYRGAARRNRPARRVVTGSEDEPGLLTLDFQGVSAAYG
                     DALLFIGYEVEGRLEVVVYPRYRFPSDDRVQGYLGYLWVEPGTPWQVVLRHVDGITEA
                     AQTNYRTAVALRSQELRAQGSRFEAAYDAIADVFRESWHGFELRLARYGEANKQGITF
                     TGRPPGPVLATWRGETYTVLVFETGSICMLSGDQRARFEHVDVALDD"
     gene            969..1526
                     /locus_tag="VFH60_10595"
     CDS             969..1526
                     /locus_tag="VFH60_10595"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HET6314277.1"
                     /translation="MSMNRPQHNVGKVSWIGAEALGLPGQRTFRVLAQGNALTAHLWL
                     EKQQLQALADAIGRMLVEIDTTQGLAIPSRTEPVSNPKPPDFPAVPDVEIHVVALGLR
                     YDPDDDLIALEAYEQETDEDSDEPPTLRCLAGRAQMQALQLNAIEVVSAGRPRCPFCG
                     TPLSQAGLPHFCPPMNGHQKLASEE"
     gene            complement(1614..>2505)
                     /locus_tag="VFH60_10600"
     CDS             complement(1614..>2505)
                     /locus_tag="VFH60_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314278.1"
                     /translation="DEPAASPATSPAPSPIVDGPRNVPASPPTGPTQPQPAPPDLFGD
                     GADKTTFLRGTGPLNPDVAGPSANELTAWLPTAGTSGYVGSTGNTGPLLHTGPLRTGP
                     FDQSVLAQAPGQPEAAGTGKQTPRGALELLPQESVAFQLGALYLTTKRVILLAPSVIR
                     SAFIRDIDAVGTFTERASGWFLFGSVLMLAGAGLSVYLSLARTTQSLELAYPWLYIID
                     PLYFGLLLLAGALLTLVRYFFHVKRTLFISVRGRPLITVSIADWNTRKLEGMDSFVNA
                     FFQIKDHMTGELDARQVEGR"
BASE COUNT          492 a          766 c          805 g          442 t
ORIGIN      
        1 atagaggact tgcagcagga catcatcatc gatgagctgg gccgcgaggt agtgatgagc
       61 cgccccaagt gtgccacact cgccctcggc acgctcaaac aggccgtgca cgactaccgg
      121 cagaagctgc ggcagcagga actggcttaa tcgtcgagag ctacgtctac atgttcgaag
      181 cgggcgcgtt ggtcgcccga tagcatgcaa atcgagcctg tttcaaatac cagcaccgtg
      241 taagtctcac cgcgccaggt cgcgaggacc ggccccggtg ggcgtcccgt gaacgtgatc
      301 ccttgcttgt tcgcctcgcc ataccgggcc aaccttagct cgaacccgtg ccagctctcg
      361 cgaaatacgt ccgcgatagc gtcataggcc gcttcaaagc ggcttccctg cgcccgcagt
      421 tcctgagagc ggagggcgac agcggtccgg tagttggtct gcgcggcttc ggtgatacca
      481 tctacgtggc gcaggaccac ctgccagggc gtcccaggct ccacccagag gtagccgagg
      541 tagccctgta cgcggtcgtc ggacggaaag cggtagcgtg ggtacaccac gacctccagc
      601 cgcccttcca cttcgtagcc gatgaagagc aaggcgtcgc cgtatgcagc cgaaacaccc
      661 tggaagtcca gtgtcaggag gcccggctcg tcctcactgc cggttacgac gcgccgggcg
      721 ggacggttac ggcgggcggc accgcggtat gagtagcttt cttgggtcat gaggataggt
      781 ccagaggggt acgggtcgca tgggtgccgg cccgattggt aattttctcc atgatatgat
      841 acaaaagcgg acggcactta acaagcaggg aggaaccggg ccgcttttct gcttcagccg
      901 ggcggcagtt cagcttgctt cggttgaccg cgtcgggccg ctaccttcgg aagccgggag
      961 tcgcacacat gagcatgaac agaccgcagc ataacgtggg caaagtcagt tggatcggtg
     1021 ccgaggcgtt gggcctcccc ggacagcgta ccttccgggt gctggcgcag ggcaatgcgc
     1081 taacggcgca cctgtggttg gaaaagcaac agcttcaggc gctcgcggac gccataggca
     1141 ggatgcttgt cgaaatcgac accacacagg gcctggcaat ccccagccgc acggagccgg
     1201 tgtccaaccc gaagcctccc gacttccccg cagtgcctga tgtggagatc catgtagtcg
     1261 ccctcggcct ccgctacgac cccgacgacg acctgatcgc cctggaagcc tacgagcagg
     1321 agactgacga ggacagcgac gagcctccca cgttgaggtg ccttgccggt cgcgcccaga
     1381 tgcaggccct gcagctcaac gcgattgagg tggtgtccgc cggcaggccg cgctgcccct
     1441 tctgtggcac ccctctcagc caagctggcc tgcctcactt ttgccctccc atgaacgggc
     1501 atcaaaagct cgcctcggaa gagtgacgcg gggaaactat gttcaggtgc aaaaagagga
     1561 cggcaagcgt cctctttttg atctctgtct gcaattattt gggcgggatc gcgctagcgg
     1621 ccttccacct gcctggcgtc tagctcgcct gtcatgtggt ccttgatttg aaagaaggcg
     1681 ttgacgaagg aatccattcc ttctagctta cgcgtattcc agtcagctat gctcacggtg
     1741 ataaggggcc ttcccctcac tgatatgaac agcgtgcgct taacgtggaa gaagtagcgc
     1801 accagcgtaa ggagcgcacc ggccagcaag aggaggccga aatatagcgg gtcgataatg
     1861 tagagccacg gatatgccag ctccagggac tgagtggtcc tggcaagcga caggtagacg
     1921 ctcaaccccg cgcccgccag catcagcacg ctgccaaaaa ggaaccagcc cgaggccctc
     1981 tcggtgaagg tgcccaccgc gtcaatgtcc ctgataaaag ccgagcgaat aaccgacggg
     2041 gccagcagaa tcacccgctt ggtggtgagg tagagcgccc caagctggaa ggcgacactt
     2101 tcctgaggaa gcagttccag cgcgccacgc ggggtctgct tgcccgtacc cgcggcctcg
     2161 ggttggccag gtgcctgggc aagcaccgac tggtcgaagg ggcctgtcct gagaggtccg
     2221 gtgtgcagga gcggaccagt attccccgtc gagccaacgt agccgctggt accggcggtg
     2281 ggaagccagg cagtcaattc gttcgcagac ggtcccgcca cgtcagggtt gagcggcccg
     2341 gtgccccgga ggaacgtagt cttgtccgcg ccatctccaa acaggtcggg cggcgcgggc
     2401 tgtggctgcg taggtcctgt gggtggggat gcggggacgt tgcgcggccc gtcaacaatg
     2461 gggctgggag ctggacttgt agcaggggac gcagcgggtt cgtcc
//
LOCUS       DASRWZ010000671         1957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_192268, whole genome shotgun sequence.
ACCESSION   DASRWZ010000671 DASRWZ010000000
VERSION     DASRWZ010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192268"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VFH60_10605"
     CDS             <1..903
                     /locus_tag="VFH60_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair protein Mre11"
                     /protein_id="HET6314279.1"
                     /translation="QKPSFHVIDTKAGPVQIIAIPWLSRSYMLSNKDFRNLDPDSLNR
                     EMINLVEGFIDQSLDKVDASLPVILTAHASVQGAVFSTEKEIMLGQDVVYPKAMLMDE
                     RIDYVAMGHIHKHQVLSQQRPPIVYPGSLERIDFGEERDAKGFVSVDISDPGPDGKRA
                     VTHTFHEVGARPFRSIKVNADCDFPTEEVLRRIEERAVDIQGAIVRLIIDTSPEHQRD
                     LRQDEIRQALNSHRPAFMAVTTNYTRAHRMRLGDQVIEQMTPRDALSIYLRSRETPPD
                     RVQNLLGYYDKLSATVAEAEKSTD"
     gene            992..>1957
                     /locus_tag="VFH60_10610"
     CDS             992..>1957
                     /locus_tag="VFH60_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314280.1"
                     /translation="MAQDDSRWQDLMKMQESANSPTNAASDQPSGQFSGDDIEFESFM
                     FDPGTGPLPTLGSLNTDPNMGVVTAVNPLAADLAAPRTGNTGPLSAPPQQPPVYAPSQ
                     PAQPVQAAPSFEFAAEEVPLPPFLGGAEPQAQQQADPEPPSWGAQPPAADEPAYEAPR
                     TAAPRGTGPLGLGAHNTGTNLAARLSQLQGEAGQSATTPRGTGPLGMRRGTGPLGGVP
                     TSGTGPLSTPTAASTAGMPGAGQGATPGPINPAYFKAPTGPLSQRMRTGPLNAASLRP
                     EPELTQQLEQAPTGMAQTGGVMGAFGTYIEQTQNDPNTWNDTTLSS"
BASE COUNT          399 a          667 c          606 g          285 t
ORIGIN      
        1 cagaagccaa gctttcatgt tattgatacg aaggcaggcc cggtacagat catcgccata
       61 ccgtggctgt cacgcagcta catgctgagc aacaaggact ttcgcaacct cgacccagac
      121 tcgctgaacc gcgagatgat aaacctggtg gagggcttca tcgaccaatc gctggataaa
      181 gtggatgcct cgctgccggt gatactgacc gcgcacgcct ccgtgcaggg ggcggtcttc
      241 tcgaccgaga aggagatcat gctggggcag gacgtggtat accccaaggc gatgctcatg
      301 gacgagcgca tcgactacgt ggcgatgggc cacatccaca agcaccaggt gctcagccag
      361 cagaggccgc caatcgtcta tcccggctcg ctagagagga tagacttcgg ggaggagcgc
      421 gacgccaagg gcttcgtgtc ggtggacatc agcgaccccg gccccgacgg caagcgcgcg
      481 gtcacgcaca ccttccacga ggtgggggcg cgaccgtttc gcagtatcaa ggtgaacgcg
      541 gactgcgact tccccaccga ggaggtgttg cgccgcatcg aggagcgcgc ggtcgatata
      601 cagggggcca tcgtgcgcct cattatcgac accagcccgg agcaccagcg cgacttgcgc
      661 caggacgaaa tccggcaggc gctcaactcg cacagaccgg ccttcatggc ggtcacgacc
      721 aactatacga gggcgcaccg catgcggctg ggcgaccagg tgatcgagca gatgacccca
      781 cgggacgccc tgtcgatcta cctgcgaagc cgcgagaccc cgcccgacag ggtacaaaac
      841 ctgctgggtt actacgacaa attgagcgcg acggtggcgg aagccgagaa atcaacggac
      901 tgatttggga tttcggattg cggatttcgg atttgctccg atatctacta aatccgaaat
      961 ccgcaatcag aaatcccaaa tggaggaata catggctcag gatgatagtc gctggcagga
     1021 cttgatgaag atgcaggagt cggcgaactc gcccaccaac gccgcgtcgg accagccttc
     1081 gggccagttc agcggcgacg acatagagtt cgagtcgttc atgttcgatc ccggcaccgg
     1141 gccgctgcct acgctgggca gcttgaacac cgaccccaac atgggcgtcg taacggcggt
     1201 taacccgctg gccgccgacc tggcagcgcc gcgcacggga aacaccggtc ccctgtccgc
     1261 gccaccccag cagccgccgg tctacgcgcc ctcgcaacct gctcagcccg tccaggccgc
     1321 gcccagcttc gaattcgcgg ccgaggaagt gccgttgccc ccgttcctgg ggggcgctga
     1381 gcctcaagcc cagcagcagg ccgacccgga gccaccgtct tggggcgcgc agcccccggc
     1441 agcggacgag ccggcatacg aagccccacg caccgctgct cctcgcggca ccggcccgct
     1501 gggcctcggc gcgcacaata ccggtacgaa cctcgccgcc cgcctctcgc agttgcaggg
     1561 ggaagcaggg caaagcgcca caacgccacg cggcacgggg ccactgggca tgcgccgggg
     1621 caccggcccc ctcgggggcg tacccacttc cggcaccggg cccctgagca ctcccaccgc
     1681 ggcttctacc gcagggatgc ctggtgcggg ccagggcgcc acaccagggc ctatcaaccc
     1741 ggcttatttt aaggcaccca ccggccctct gagccagcgc atgcgcaccg gccccctcaa
     1801 cgcggccagc ctgcgcccgg agccggagtt gacacagcag ctggagcagg cacctaccgg
     1861 catggcgcag accggtgggg taatgggcgc gttcggtacg tacatcgagc agactcagaa
     1921 cgatcccaac acctggaacg ataccacgct ctcctcg
//
LOCUS       DASRWZ010000672         6897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_534943, whole genome shotgun sequence.
ACCESSION   DASRWZ010000672 DASRWZ010000000
VERSION     DASRWZ010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534943"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFH60_10615"
     CDS             complement(<1..761)
                     /locus_tag="VFH60_10615"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET6314281.1"
                     /translation="MVMKVGINGFGRIGRQVFKALRDYYPDEIQVVAVNDLTDNNTLA
                     HLLRYDSNYGPLDADVSATEDTIIVDGDEIRAFAERDPATLPWGDIGAEIVIESTGVF
                     TDAERARAHLTGGAKKVIITAPAKGEDITVVMGVNHHRYDPREHTIVSNASCTTNCLA
                     PVAKVLNDTIGIERGLMTTIHAYTKDQKILDQIHSDMRRARAAAMSMIPTTTGAARAV
                     GEVLPELKGKLDGSSVRVPTPNVSLVDLTFTPPRDT"
     gene            complement(962..2386)
                     /locus_tag="VFH60_10620"
     CDS             complement(962..2386)
                     /locus_tag="VFH60_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HET6314282.1"
                     /translation="MANVGGMGNMERWLPSGYGFRRWALVFALGVAIVGLALAMGVTN
                     LYRTYVLPQSGTPFIYAVTLQYIPHPWRESLVGIVGLATGLLGARGMFRAAGRAAAKG
                     RQYEMPVLAPLRAARRAGGPRIVAIGGGTGMPTLLRGLKEVTDNLAAVVNVVDDGGSS
                     GRLRRDMGVLPPGDFRNNLVALSESEPLLSELFQHRFTGPGELEGHSFGNIFLVAMNE
                     ITGNFEQAIRECSQVLNVRGKVLPASLTNIELYAELQNGQVVRGESVIPRAPSPVKRI
                     YMEPAHAPGYPEAIAEILGAELIVLGPGSLYTSIMPPLLVADIARAVKNNKTAVKVYI
                     CNVATQPGETDGYGVVEHVRALHNHIGPGLFDYVIYNVNRGVEKQIKPEWGVTPVTLD
                     KDASARFPNLEFVPADVVRDDNPLRHDPQKLTDAVTRLYDNRGGRGAPGRVRDRDRAA
                     THSRNGTGYADIHETEELEAGAEK"
     gene            complement(2513..3766)
                     /locus_tag="VFH60_10625"
     CDS             complement(2513..3766)
                     /locus_tag="VFH60_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET6314283.1"
                     /translation="MPLYLDISIALVVFMGLASGGILSLVLMSERRQQTNFSIMLALS
                     LFLALGGGTGMIAALPNVASSLTNMLILLVLSMALGYTLTAFSVLSPKRKKWSLEAPQ
                     AKARAERTAVILLMPGEPPEYSVWSAARRLELADDPKDVPPILLRPFYMRDLKHKYAA
                     LGSSPYREHQHRLAEEIRSRLESGYSVYEAYYSDHPTLAEAAVEAIEEGARRIIVAHV
                     RVTDPPDPVMQGELWEGFDLDNLGVKVTQVGPVWVSSLLPQIYVRRVIEAIPQAGNTP
                     DDVGLLLVGRGHATEGESAQARQEQEESFQRKVRQALLRVGFGEDRVVIGWVRRSPNC
                     AEALQALVVAGCTSVYVIPSAFVADGITTLFDIPAQVQALADERGVKLSYLPAWNADG
                     LAAEEIAVYVRAVSAPALMELGIRN"
     gene            4283..5005
                     /locus_tag="VFH60_10630"
     CDS             4283..5005
                     /locus_tag="VFH60_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314284.1"
                     /translation="MSTRVRPLKPQIEDSTQRGLPQVSYYPPLVVTLLASVFLLAVLV
                     VLTFITGLAAITQVGRLGDNGSSGAGLTMFACGLVSLLCLVATVFFVWSVIKGVRDLL
                     TPLHYTRGVVADKRVIGGRRAGTWLGVFPNYSGPDLTIASQAEAPRSPGSGARSGDTP
                     GQGSTATHRSSGYLSADRISADEATETTTRRIFRVDAASHAVMEAGDEVLVAHSRFLE
                     HIFYVARLNGDEWESYKNKALI"
     gene            complement(5039..6487)
                     /gene="gltX"
                     /locus_tag="VFH60_10635"
     CDS             complement(5039..6487)
                     /gene="gltX"
                     /locus_tag="VFH60_10635"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET6314285.1"
                     /translation="MADKVRLRIAPSPTGDPHVGTAYMALFNKAFAHRHGGKFILRIE
                     DTDQSRYNEGSEREIIESLRWLDLLPDEGPGIGGDYGPYVQTQRRELYARHAVELLEK
                     GKAYRCFCTPQRLEEMRREQAARKEPPGYDRRCFRLSPEEIQANLDAELPFTVRMLVP
                     DEGSTAFTDLIRGEISFENRVLDDQIILKSDGLPTYHLAVVVDDHYMEISHIVRAEEW
                     ISSTPKHVLLFQALGWEMPKFAHLPLLRNADRTKISKRKNNTSIKWYREQGYLPEAMV
                     NFLGLMGWSMPNGEETFTFEDVQREFTFERVTTSGPVFDLVKLTAINGKYIRLLSDGE
                     LYERLVPYLPEGLDEARVRKAVPVVKDRLTRLGEFAELTGYLFGPPPVYDAATLVPRK
                     NTPEAARAALEKTRQLLGDLPEPWTHEEWEGGMRALAAELGVKAGDAFMVLRVAVTGS
                     QTSPPLFESIEILGKEETLRRVDAGLAKLGAG"
BASE COUNT         1168 a         2287 c         2080 g         1362 t
ORIGIN      
        1 gtgtcacggg gcggcgtgaa ggtaaggtcg acgaggctga cgttgggcgt cggcacgcgc
       61 accgaagagc cgtcgagctt gcctttcagc tccggcagca cctcgccgac cgcgcgggcg
      121 gcgccggtcg tggtcgggat catcgacatg gcggcggcgc gcgcgcggcg catgtcggag
      181 tggatctggt cgaggatctt ctggtctttc gtgtaggcgt ggatggtcgt catcaggccg
      241 cgctcgatgc cgatcgtgtc gttcagtacc ttggcgacgg gcgcgaggca gttggtggtg
      301 cacgatgcgt tggagacgat ggtgtgctct cgcgggtcgt agcggtggtg gttcacgccc
      361 atgaccaccg tgatgtcctc ccctttggcc ggggcggtga tgatgacctt cttcgcccca
      421 cccgtcagat gcgcccgcgc cctctcggca tcggtgaaca cccccgtcga ctcgattacg
      481 atctcggccc cgatgtcgcc ccacggcagc gtggcggggt cgcgctccgc gaacgccctg
      541 atctcgtcgc cgtccacaat gatcgtgtcc tcggtggctg agacatcggc atcgagcggg
      601 ccgtagttgc tgtcataccg cagcaggtgg gccagggtgt tattgtcggt caggtcgttg
      661 actgccacca cctggatttc gtcgggatag tagtcccgca acgccttgaa cacctgccgc
      721 ccgatgcggc cgaaaccgtt gataccgacc ttcattacca ttgcgctatg ctcctttcga
      781 acgcgaacgt tcgctcccaa agtgacgagt gacgagtgac gagtaccgag tacagagtac
      841 cgaggggcac ccatcgaaac aaacattaaa ctttgcgcgt tctttgcgtc ctttgcgtcc
      901 cttcggctgc gctcagggca tgctttgcgt ttccgtctct ctactcggta ctcggcactc
      961 gttacttttc tgctcctgct tccaactcct ccgtctcgtg gatgtccgca taacctgtgc
     1021 cgttgcgcga gtgtgtagcg gcgcggtctc gatctcgcac gcggccgggc gcgcctcttc
     1081 cgccccggtt gtcgtagagc cgggtgacgg catcggtcag cttctggggg tcgtgacgca
     1141 gcggattgtc gtcgcgcact acgtcggcgg gcacgaactc caggttgggg aagcgggccg
     1201 acgcgtcttt gtccagcgtg acgggggtaa cgccccactc cggcttgatc tgcttctcca
     1261 ccccccggtt gacgttgtag atgacgtaat cgaataggcc cggcccgatg tggttgtgca
     1321 gcgcccgcac gtgctccacc accccgtagc cgtcggtctc ccccggctgg gtggcgacgt
     1381 tgcagatgta cactttcacg gccgtcttgt tgttcttgac cgcccgcgcg atgtcagcca
     1441 ccagtagggg cggcatgatg ctggtgtaga gcgaccccgg ccccagcacg attagctcgg
     1501 cacccagtat ctcggcaatc gcttcggggt agccgggtgc gtgcgccggt tccatgtata
     1561 tgcgcttgac gggcgacggg gcgcgcggta tgaccgactc cccgcgcacc acctggccgt
     1621 tctgtagctc ggcgtacaac tcgatgttgg tgagggaggc gggcagcacc ttgccgcgca
     1681 cgttgagcac ctgggagcac tcgcgtatcg cctgctcgaa gttgcccgtg atttcgttca
     1741 tggcgaccaa gaagatgttg ccgaaggagt ggccttcaag ctcgcccggc ccggtgaagc
     1801 ggtgctggaa cagctccgaa agcagcggct ccgactcgga gagggccacc aggttgttgc
     1861 ggaagtcgcc ggggggcagc acgcccatgt cgcgtcgcag cctgccggac gagccgccgt
     1921 cgtccacaac gttcaccacg gccgcgaggt tgtcggtgac ttccttgagg ccgcgcagca
     1981 gggtgggcat gcccgtgccg ccgccaatag ccactatgcg cggcccgccc gcccgcctcg
     2041 ccgccctgag gggcgctagc accggcatct cgtactgcct gcccttggcg gcggctcgtc
     2101 cggcggcgcg gaacatgccc ctcgccccca gcaggcccgt cgccaggccg acaatgccga
     2161 ccagactctc ccgccagggg tgagggatgt actgcaaggt caccgcgtaa ataaagggag
     2221 tgccgctctg ggggagcacg taggtgcggt acaggttggt cacgcccatc gcgagggcca
     2281 ggcccactat tgccacgccc agggcgaaca ccagcgccca tcgccggaag ccgtagccgc
     2341 tggggagcca ccgctccata ttacccatcc cacctacgtt cgccattcag tccccactct
     2401 ccgaacatgg taaatcagcc cattataacc gatttggaaa ttggaatttc gaaattcgaa
     2461 tttagtaggt atcccagcaa attcgcaatt cgcaattcca atttccaaat tcctagttcc
     2521 gtattcccag ttccatcagg gccggtgcgc tcacggcgcg gacgtaaacg gctatctctt
     2581 cggcggccag gccgtcggcg ttccaggcag gcaggtagct gagcttgacg cctcgctcgt
     2641 ccgctagagc ctggacctgc gcggggatgt cgaacagggt ggtgatgccg tcggccacga
     2701 acgcggacgg tattacatag acgctcgtac agcccgccac caccagcgct tgcaaggctt
     2761 cggcgcagtt gggggagcga cgcacccagc ctatcaccac gcggtcctca ccgaacccca
     2821 cccgcaacag ggcctggcgc accttgcgct ggaagctttc ttcttgctcc tgcctcgcct
     2881 gcgccgactc gccttccgtg gcgtgccccc tgcccaccag cagcaggccc acgtcgtcgg
     2941 gggtgttgcc cgcctggggg atggcctcta tcacccgccg cacgtagatc tggggcagca
     3001 agctggacac ccacaccggc cctacctgcg tcacctttac gcccaggttg tcgagatcga
     3061 agccttccca caattcgccc tgcatgaccg ggtcgggcgg gtcggtcacg cggacgtgag
     3121 ccacgatgat gcgtcgcgcg ccttcctcga tagcctccac ggcagcctcg gccagtgtgg
     3181 ggtggtcgct gtagtatgcc tcgtaaacgc tgtagcccga ctcgagccgc gaccgtatct
     3241 cctcggcgag ccggtgctgg tgctcgcgat aggggctgct accgagggcc gcgtacttgt
     3301 gcttgaggtc gcgcatgtag aaggggcgca gcagaatggg gggtacgtcc ttggggtcgt
     3361 cggccagttc gagacgccgg gccgcgctcc ataccgagta ttcgggcggc tcgccgggca
     3421 tgaggagtat cacggcggtg cgctcggcac gcgctttcgc ctggggagct tcgaggctcc
     3481 acttcttcct cttgggtgaa agcaccgaga aagccgtgag ggtgtaaccc agggccatcg
     3541 agagcaccag caatatgagc atgttggtga ggctggaggc gacgttgggc agcgcggcta
     3601 tcatgcccgt gccgccacca agcgcgagga acagcgagag ggccagcatt atgctgaagt
     3661 tggtctgttg tcgccgctcc gacatgagca ccagcgacaa tatgccgccg ctcgcaaggc
     3721 ccatgaacac gaccagcgcg atgctgatgt ccaggtaaag tggcactcac tctctccgcg
     3781 aaagacatgt ccccacagtc gccggtctgc cctccaggct ggcacattat agcacagtgc
     3841 cgagtgccga gtgccgagtg ccgagtgccg agtagtggtc agtggctagt ggttagttgt
     3901 ggctcgcaaa tgttggagac tccctgtcat gaatgccctg tggctaaagc cacgggctta
     3961 ccagagacga aatctccttc ggagactaca tgatgttgct tagtccccgc aggggactct
     4021 gccccttgtc agcccgtggc tttatccaca gggcgtcctc cactgggcta tcccaggccc
     4081 tctccaggac aaactgctac tcctgtgcct ggcatcccaa ccctttgaac cttacgccgt
     4141 ccaaccctgt atctctagta gccttcgcaa acacgcaaac acgcaaacac gcaaacacgc
     4201 aaacacgcta accactaacc actaaccact aaccactggc aaccgaccac tgacaactga
     4261 ccactgtgct ataatcaagc ccatgagcac gcgcgttcgc ccattaaaac cgcaaatcga
     4321 ggatagtacc cagcgggggc tgccccaggt cagctattac ccgccgctgg tcgtcaccct
     4381 gctggcgtcc gtcttcctgc tggccgtgct ggtggtgctc accttcatca ccgggctggc
     4441 agcgattacc caggtcggga gactcgggga caacgggtcg tccggggcgg ggttgacgat
     4501 gtttgcttgc gggctggtga gcctgctttg cctggttgcc accgttttct tcgtatggag
     4561 cgtcatcaag ggcgtgcgcg acctgctcac cccgctccac tacacgcggg gcgtcgtggc
     4621 cgacaagcgc gtaatcggcg ggcgcagggc cgggacctgg ctgggcgtct tccccaacta
     4681 ctcaggcccc gacctgacta ttgcctccca ggcagaagcc ccccggtccc caggctccgg
     4741 cgctcgttcc ggcgacacac cgggccaagg ctctaccgcc acccataggt cgagcggcta
     4801 cctctccgcc gaccgtatct cggctgacga ggccacggag accactacgc gccgcatctt
     4861 ccgcgtcgat gcggcctccc atgccgtcat ggaagcaggc gatgaggtgc tggtggccca
     4921 ctcccgcttc ctcgagcata tcttctacgt ggctcgtctc aacggtgacg agtgggagtc
     4981 ctacaagaac aaagcgctga tctagccgaa ctcgtaattc gtaattcgta attcgtaatt
     5041 acccagcccc cagcttcgcc agccccgcgt ccaccctccg caacgtctcc tctttgccca
     5101 ggatttcgat gctctcgaac agcggcggcg acgtctggct gcccgtgacg gccactcgca
     5161 gcaccatgaa ggcgtccccg gccttcaccc caagctccgc cgccagcgcg cgcatgcccc
     5221 cctcccactc ctcgtgcgtc cagggttccg gcaggtcccc aagtagctgc cttgtcttct
     5281 caagcgcggc gcgtgcggct tccggcgtgt tcttcctggg gaccaacgta gcggcgtcat
     5341 agacgggcgg cggcccgaaa aggtagccgg taagctccgc gaactccccc aggcgcgtca
     5401 ggcggtcctt tactaccggc acggccttgc gcacgcgcgc ctcgtccagc ccctcaggca
     5461 ggtacggcac caggcgctca tatagctcgc cgtccgagag tagacggatg tacttgccgt
     5521 tgatcgcggt gagcttcacc aggtcgaaca ccgggccgct ggtggtcacg cgctcgaaag
     5581 tgaactctcg ctgcacgtcc tcgaacgtga acgtctcctc gccgttgggc atcgaccagc
     5641 ccatcagccc caggaagttg accatcgcct ccggcaggta gccctgctcc cggtaccact
     5701 taatcgacgt gttgttcttg cgcttcgaga tcttcgtgcg gtcggcgtta cgcagcaatg
     5761 gcaggtgggc gaacttcggc atctcccagc ccagcgcctg aaacaacagc acgtgcttgg
     5821 gggtgctgct tatccattcc tcggcccgca cgatgtggct gatctccatg tagtgatcgt
     5881 ccaccaccac ggccaggtga taggtgggca gcccgtcaga cttgaggata atctggtcgt
     5941 ccagcacccg gttctcgaag ctgatctcac cacggatcaa gtcggtgaac gcagtggagc
     6001 cttcgtcggg cacgagcatg cgcaccgtga acggcagttc ggcgtccagg ttggcctgta
     6061 tctcttcggg ggagaggcga aagcaccgcc ggtcgtagcc gggtggctcc ttgcgcgcgg
     6121 cctgctcgcg gcgcatctcc tccagccgct gcggcgtgca gaagcacctg tatgctttgc
     6181 ccttctccag cagttcgacg gcgtgcctgg cgtaaagctc gcggcgctgc gtctgcacgt
     6241 aagggccgta atcgcccccg atgcccggcc cctcgtcggg caacaggtcg agccagcgca
     6301 ggctctcgat tatctcccgc tccgagcctt cgttgtagcg gctctggtcg gtgtcctcga
     6361 tgcgaaggat gaacttgccg ccgtgcctgt gcgcgaacgc cttgttgaac agggccatgt
     6421 aagccgtgcc tacgtgcggg tcgccggtgg gcgacggcgc gatacgcaag cgtactttgt
     6481 ctgccattct aatgctctcc cttgttggaa atctccacta tagccgctgc cgcctgccct
     6541 tcctctatcg tgcgccttcc atctttaaga attccccttg caactctcct agctcgcgga
     6601 actcctgttc cgtcagttcg tgcagggcct gtgcgtgcct cttcagcacc aataccagcc
     6661 aacccttcat gctacaggga taagcgtgct ccaccagcca gtgcgcggtc tcgtagatgg
     6721 tcggccccgg cgagatgcgc tactcccccg atatcgattt gcacgtgagg caattcaacc
     6781 caggctccca acgatgctac cttgcacgct cccgctcgcc cggttatgtg gctgactatt
     6841 actgtaaatg agaatgccct cagttagcaa gtggcacaaa aagtagagga ggcgcat
//
LOCUS       DASRWZ010000673         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1220307, whole genome shotgun sequence.
ACCESSION   DASRWZ010000673 DASRWZ010000000
VERSION     DASRWZ010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220307"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VFH60_10640"
     CDS             complement(<1..763)
                     /locus_tag="VFH60_10640"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6314286.1"
                     /translation="MNTWRAQPVTARLFLAVLSQAPIVLVVWLWSRLSERVRVVEYDG
                     GVFLLGLALALSLFLYGVFCYLAYCAFSISYSLDRDFLAMRCGGAVVRVPLSAVRSVH
                     GAGETPGGPVGVRWKGAAGVVPGYVVGEGRSAQLGRVHSIATLPAQRQVWVVARGVAY
                     GLSPEYPERFVQLLNARLEDEDESPGPAPRVRRSRVLDIGLGLWGDRVVRTLFLAGLA
                     LNVLLWGYLSLVYTELPSRVAMHWNAQAQVDRIGEP"
     gene            complement(865..1842)
                     /locus_tag="VFH60_10645"
     CDS             complement(865..1842)
                     /locus_tag="VFH60_10645"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET6314287.1"
                     /translation="MNNESPLLCYFEGKVMPLSEAKVGILTHALSYGTGCFEGIRAYF
                     NEEHGQLYVFRGLEHYQRLDASACILLMSLPDTPQALLDRTLELLKDSGLYTDSYIRP
                     MMYKADEIIGVRLHDLRSELYITVQPFGNYIDIHRALNVGTSTWRRIDDNMIPARGKV
                     SGAYVNSAFAKTEAQLNGFDEAIMLDHDGHVSEGSAENLFMLRGGMVYTPPVTANILE
                     GITRNTVMTLLREEMGLTVVEREIDRTELYVCDELILCGTGAQIAPVGTIDHRKIGDG
                     SVGQLGRALQDLYFEVVRNNVPKYSHWCTPVHTEVRRRNQQEQLAIANA"
     gene            2061..2468
                     /locus_tag="VFH60_10650"
     CDS             2061..2468
                     /locus_tag="VFH60_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6314288.1"
                     /translation="MGKYTSLARRIKELRQVANFTQQDLSRASGLSRSYISRLEMGDI
                     ALPSRDKLRALAVALGASLDDLLQAAGFLDAVSDTSNLPDIKAYLRRKYGIYNPQVLQ
                     AIETIVTNVQSTNGATEHAPAGAGGLQDVAAPN"
BASE COUNT          585 a          888 c          785 g          488 t
ORIGIN      
        1 aaggctcgcc gatcctatcg acctgtgcct gggcgttcca gtgcatggct accctgctgg
       61 gcagttcggt atagactagg ctcaagtagc cccagagcag gacgttcagc gccaggcccg
      121 ccaggaagag cgtgcgcacc accctgtcac cccaaagccc caggcctatg tccagcaccc
      181 tggaacgcct cacgcgcggg gccgggccgg gggactcatc ctcgtcctcc agccgggcgt
      241 tcagcagttg cacgaaccgc tccgggtact cgggcgagag gccatacgcc accccacgcg
      301 ctaccaccca cacctggcgt tgagcgggca gcgtggctat tgaatgcacc cgccccagtt
      361 gcgcgctccg cccctccccc accacgtacc ccggcaccac gcccgccgcg cccttccatc
      421 gcacccccac cggaccgccc ggcgtctcac ccgcgccatg caccgaccgg accgcgctca
      481 gaggtacgcg cacgacggcc ccgccgcacc gcatcgccag aaagtcgcgg tcgaggctat
      541 aggagatgct gaaggcgcag taggcgaggt agcagaacac cccgtagagg aagagcgaga
      601 gggccagggc cagcccaagc aggaacaccc caccgtcata ctcgacgacc cgcacgcgct
      661 ctgaaagcct ggaccacagc cagacgacaa gtacaatagg agcctgcgac agcacagcca
      721 ggaagagccg tgccgttaca ggctgcgcgc gccacgtgtt catacgggac aaatataaca
      781 cgtaacgcgt aacacgtcag gtctgttcgc acagacaccc gtgttacgcg ttactttcgc
      841 ctattccgtg ttacgcatta cgtgttacgc gttagctatg gcaagctgct cctgctggtt
      901 gcgccggcgc acctcggtgt gcacgggcgt gcaccagtgc gagtacttgg gcacgttgtt
      961 gcgcaccacc tcgaagtaga ggtcttgcaa cgcccggccc aactgcccaa cgctgccatc
     1021 gccgatcttg cggtggtcta tcgtacctac cggggcgatt tgagcgcccg tgccgcacaa
     1081 tatcagctcg tcgcacacgt atagctcggt gcggtcgatc tcccgctcca caaccgtgag
     1141 gcccatctcc tcgcgcagca gcgtcatcac cgtgttgcgg gtgatgcctt cgagtatgtt
     1201 ggccgtcacg ggcggggtgt agaccatacc gccgcgcagc atgaacaggt tctccgccga
     1261 gccttccgac acgtgcccgt cgtggtccag catgatcgct tcatcgaagc cgttgagttg
     1321 cgcctccgtc ttggcgaagg ccgaattgac gtacgcgccc gacaccttgc cgcgggccgg
     1381 tatcatgttg tcatcgatgc gccgccacgt cgaggtgccc acgttgaggg cgcggtggat
     1441 gtcgatgtag ttgccgaaag gttgtacggt tatgtaaagc tcactgcgca ggtcgtgcag
     1501 gcgcacgccg attatctcgt cggccttgta catcatcggg cggatgtagg agtcggtgta
     1561 gaggccgcta tctttgagca gttccaacgt tctgtcgagc agggcctggg gggtgtcggg
     1621 cagcgacatg agcaggatac aagcggaggc atccaggcgc tggtagtgct ccagcccacg
     1681 gaagacgtaa agctggccgt gctcttcgtt gaaatacgcg cggatgccct cgaagcagcc
     1741 cgtgccgtag ctcaacgcgt gggtgaggat gccgaccttt gcctcgctca acggcattac
     1801 cttaccctcg aagtaacaaa gtagcggcga ttcgttgttc atgtcgtgtt ctcctattgg
     1861 ggggaaacgg caccggacta cgttgtccgg accgtaggcg actcctcgcc gcccatccga
     1921 ttatatcaca tgccgagact tcggtcagtt caaacagtca tgtaaacgcc cccgacaggt
     1981 gactaactgt aaacaattag gcaacctcac gcctgggtcc aacttgcccc ccgcctacgc
     2041 tataggttat agtagacact atgggaaaat acacatcact cgcgcggcga atcaaggaac
     2101 ttcgccaggt agcgaacttc acgcaacagg acctgagcag agccagcggg cttagcaggt
     2161 cgtacatatc aagacttgag atgggcgaca tcgccctgcc ttcacgagac aaactgcgcg
     2221 ccctggccgt tgccctgggc gcgagcctgg acgatctttt gcaagccgcc ggtttcctcg
     2281 acgccgtttc cgacacctcc aacctccccg acatcaaggc ctacctgcgg aggaaatacg
     2341 gcatctacaa cccccaggtg ctacaggcta tcgagacgat tgtcaccaac gtacaaagca
     2401 ccaacggcgc aacggagcac gccccggcag gcgcgggcgg cctgcaagat gtggctgccc
     2461 ccaactaaca agcggcggca gagaaaagta gtagcccaaa ggtaccatcc ggcctagtac
     2521 cagggttagt actccagggg ggccaaaaag gtacctttag gccatataca cgattcggcc
     2581 gctgaggtaa tataagggca gaagaagtgt aagaagttgc taaatcctgc tcctcctggt
     2641 tcgaggcctc gaaagaggcc tcttttcttt gcccaaaaca cacaggagga gacggaacca
     2701 ccaagacgcc aagaacgcca aggaccacgc caagatttaa cgtttt
//
LOCUS       DASRWZ010000674         9678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_808778, whole genome shotgun sequence.
ACCESSION   DASRWZ010000674 DASRWZ010000000
VERSION     DASRWZ010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808778"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..632)
                     /locus_tag="VFH60_10655"
     CDS             complement(81..632)
                     /locus_tag="VFH60_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314289.1"
                     /translation="MIAKKLATFLSLAALVLVLAACDSQPATPTTPGVSDSGTPTPGD
                     DIPISPAETATIPANATTTPGVLLTFTKTGGFAGFNTTLTVQQTGEYTLAERGQQPKS
                     GKLDDAKLNDLKTQLDVVRGLGDVEDKYDEGNVADDIYRTVMFDQNGTLKSVTVAEIG
                     GQGLAPEELQKLIATINSIVETQ"
     gene            complement(1048..3783)
                     /locus_tag="VFH60_10660"
     CDS             complement(1048..3783)
                     /locus_tag="VFH60_10660"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET6314290.1"
                     /translation="MSDTTLAEDSAKQGDEMAKAYSHEAVERRWYDWWEGQGYFTASR
                     DPNRKPYTIIMPPPNVTGELHMGHALTNAVEDILIRWRRMQGYEALYLPGEDHAGISG
                     QYVVEKELAKEELSRHDIGREKFLERAWEWMNEYRPRIRYQLRRLGASCDWTRERFTL
                     DPGPVSAVRTVFKRLYDKGLIYKGERIVNWCPRCMTTLSDLEVEPEEEQGHLWTIRYP
                     LAGDPSQGIEVATTRPETMLGDTAVAVHPDDERWQHLIGKEVLLPIMQRPIPVIADTG
                     VDKEFGTGAVKITPGHDPLDFEIGQRHDLPIINLLNLDGTLNPNAGLYAGLGVEEARA
                     GVGKQLEDEGYLIRIDAHTHSVPHCERCGTVLEPIVSDQWFVKMQPLAAPAIEVVRRG
                     EIRIVPERFTKVYYHWMQNIRDWPISRQLWWGHRIPVWYCDNGHQFAAIEDKAAACEI
                     CGSTDLRQDEDVLDTWFSSGLWPFSTLGWPEETEDFKYFYPTDVMETGYDILFFWVAR
                     MIFQGIEHTGRIPFHTVYLHGLVRDETGRKMSKSWGNVVDPIGLADRYGSDALRLSLI
                     TNSSPGNDQKFSDQRTEQAGHFANKLWNATRFVLAQTMDDNNPKSKIQNPKSLADRWI
                     LSRYSRTVSQVTQMLEDYQLGEAGKVLQEFVWSEYCDWYIEAAKGALRSEDAETRGAT
                     LAVARTVLDGILRLLHPYMPFVTEELWQNLHGWPSRAESLGKREQSIIVAEWPAALAT
                     DEEAERDFDTVIEIIRQIRNARAETIKDAPDNLKGEMARRKIEAIIGGGSRAPMLQQE
                     ADTLARLAQLDAAKLTITESISGEQRPEQAATLVIGEIEVVLPMSGLVDTGAERKRLR
                     SEAESTQAEIERAQALLSNDQFTSRAPAQVVERERARLSAAQERLSKLNERLATL"
     gene            complement(4030..5976)
                     /locus_tag="VFH60_10665"
     CDS             complement(4030..5976)
                     /locus_tag="VFH60_10665"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HET6314291.1"
                     /translation="MAGNGTTSVKSNHPFLVRLREARPIVCDGAMGTMLYSMGVPANS
                     CFDESNLTQPDLVRKVHETYIKAGAEIIETNTYGANRHKLAGYDLADKVGQINKRGAR
                     LARDVREVSGQPVMVAGAVGPLGKPLKPVGLIPALEARTVFREQIEALLEGGVDLIIL
                     ETIADLEEMRQALIAAHDACDLPVVAQMTFDGDGRTQMGNTPAEVARALEELGADVIG
                     ANCSTGPQRMSRVISEMSAVVKTPLSAQPNAGWPEMVGDRIVYVSTPDYVASFASRMM
                     AAGARLLGGCCGTTPAHIQAIAATVAELEATGVADGAATQEPAQPVRVSEPEPPMLRE
                     AAPLELSDTSASVHTEVGSFGWKLGKEFVVSIELAPPRGVNPSKLLAGAHMLKAKGVD
                     AVNVTDSALGRASMSALAVSYLVQQQVNMGVIVHFTTRDRTLMGIQAELLGAHAMGLR
                     HILALTGDAPNPVDRLGSTAVFDIDAIGLIKLLTRLNDGLDLADNSIGGTTDFLIGCA
                     FNPTARDLPLEIDRLGQKLEAGAHFVMTQPVYDLDLVARTLESVQSFNARVLLGILPL
                     QSFRHAEFLHNEVPGIVIPDFVLKRMEEAGANGRQAGVEMAQEILKYAYNRYAGAYLM
                     PSFGRYELCASVLDVLPDFAPVGT"
     gene            complement(6528..7289)
                     /gene="rsmG"
                     /locus_tag="VFH60_10670"
     CDS             complement(6528..7289)
                     /gene="rsmG"
                     /locus_tag="VFH60_10670"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET6314292.1"
                     /translation="METRLAILQEGARQLGFTLTPRQLDQFQLYYDTLVDWNSRINLT
                     AITDYDLVQVRHFLDSLTVAAALLSELRGTQPGLAAPPAAFRLIDIGTGAGFPGVPLK
                     ILWPHIDLTLNDSVGKKTSFLQELARAVALEDVRVVTARAEELGQDKLHRQRYDLVTA
                     RAVSALPVLCEYCLPLAKVGGHLLAPKKGDISQELSEGKQAAKLLGGRLGKTYPFTLP
                     GDQEPRTVISVRKVASTPPGYPRRVGLAKTKPLGR"
     gene            complement(7293..7907)
                     /locus_tag="VFH60_10675"
     CDS             complement(7293..7907)
                     /locus_tag="VFH60_10675"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6314293.1"
                     /translation="MSGNGDSHILQKQGKPLGEQLGWHLLGTTYPFVSPWITLRQDRV
                     RLRGKDEPIEFNYQEHPGAVGVVPVTKQGEVVLIRQYRYTIDAWSLEVPAGGMHDADG
                     ATHEEVAGKELEQEIGATCGEMLYIGCYYTTVGQSDQAYHIYLALDVALEREQSTEET
                     EQIEVRPVPAAEALAMCRDGRIQDGNAALALLLCEGHLRERGYI"
     gene            complement(7904..8272)
                     /locus_tag="VFH60_10680"
     CDS             complement(7904..8272)
                     /locus_tag="VFH60_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6314294.1"
                     /translation="MPVIGLDHVQVAAPRREGVEQQARDFYAGLLGLRELEKPDILKP
                     KGGVWFSLGAGELHVGLEDQFSPALKAHPALHVRDLPALRRSLEEAGIVIAEAEPIPG
                     VSRFYVSDPFGNRIELMERA"
     gene            complement(8278..9342)
                     /gene="fni"
                     /locus_tag="VFH60_10685"
     CDS             complement(8278..9342)
                     /gene="fni"
                     /locus_tag="VFH60_10685"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET6314295.1"
                     /translation="MLEAQYNAPAIAMSIENRKADHIRICLEEDVSGRGITTGLERYR
                     FVHQALPELDLAAVDTSLRLWLHTLRLPLLISSMTGGAPRAEEINRNLAIAAQELGLA
                     MGVGSQRAALKDPQLAPTYQVRRYAPDVPLFANLGAVQLNYAYTLDECKRAVDMIEAD
                     ALILHLNAIQEAVQPEGDTNFAGLAAKIAQVCRGLDVPVIVKEVGWGISEDAATLLWE
                     AGVSAIDAAGAGGTSWSQVELHRIPAERDRLVAEAFADWGIPTADCLKALTLNLPQGG
                     KVFASGGIRNGIDAAKALALGADLVGIARPFLKAAAISAEATVELGGVLRDQLRIAMF
                     GIGAADLEALRFTPHLVEKG"
BASE COUNT         1558 a         2878 c         3334 g         1908 t
ORIGIN      
        1 gctgccgcat gtcgcaggta gccctgacag tctgacgatg ccttggcgtg agccgtggca
       61 cctttgcaaa gctgcccgtg ctactgcgtc tcaacgatgc tgttgatggt ggcgattagc
      121 ttttgaagct cctcaggagc gaggccctgg cccccgatct cggccaccgt cacggacttg
      181 agtgtgccgt tctggtcgaa cattacggtc ctgtagatgt cgtcggctac gttgccctca
      241 tcgtatttat cctctacatc cccaaggccg cgtaccacgt caagctgcgt tttgaggtcg
      301 ttcagcttgg cgtcgtccag cttgccggac ttgggctgct gtccgcgctc cgccagggtg
      361 tactcgcccg tctgctgcac ggtgagggtg gtattgaagc ccgcgaagcc cccggtcttt
      421 gtgaaggtga gcaacacgcc aggggtggta gtcgcgttgg ccgggatcgt agcggtctcg
      481 gcgggggaga taggaatgtc gtcgcctggg gtgggcgtgc cgctatcgga tacgccgggc
      541 gtggtcgggg tggcgggctg gctgtcgcag gcggcaagca cgagcacgag ggctgccagc
      601 gagaggaagg tggcaagttt tttggctatc atgtgggttc tccatgctgc taggaagtag
      661 tcgttgctca tctttaagca atatcgaggc cctgcggcgg accataaggc tacaaatagt
      721 ggaggcgcag gtatctcgtc agaggctacg ctcacttgct cagttgggct accacggtgc
      781 tgaacgcgca ggaccacaca tgcccctgtc atcctgagtg tagcgaagga tctaagatgc
      841 tgccttcggt ttgctgccga attaccgacc accagttccc tggaagctca acagcatcgc
      901 ctgaccaact tcttgagatc cttcgctacg ctcaggatga cagggtggga cttggccccg
      961 caaaccgaaa cgtactgccg aaaggaggag tggttcttta cggaccactc ctcaactcaa
     1021 aactcagcac tcagcactca aaacttccta cagcgttgcc agccgctcgt tcagcttcga
     1081 cagcctctcc tgggccgcgg acagcctggc tcgctctcgc tcgaccacct gggcaggggc
     1141 gcgggaggtg aactggtcgt tggagagcag agcctgggcg cgctcgatct cggcctgcgt
     1201 agactcggct tccgatcgca gccgcttccg ctcggccccg gtgtccacca ggcccgacat
     1261 cggcagcacc acctcgattt cgcctatcac cagggtggcg gcttgttcgg gccgctgctc
     1321 accgctgatg ctctccgtga tggtgagctt ggccgcatct agttgggcca ggcgggccag
     1381 ggtgtcggcc tcctgttgca gcatcggggc acgcgagcct ccaccgatga ttgcctcgat
     1441 cttgcgccgg gccatctcgc ccttcaggtt gtcgggcgcg tccttgatag tctcggcgcg
     1501 ggcgttgcga atctggcgga tgatctcgat cactgtgtcg aagtctcgct cggcctcttc
     1561 atcggtagcc agggctgcgg gccactcggc cacgatgata ctttgctccc gcttgcccag
     1621 cgactcggcg cgggagggcc agccgtgcag gttctgccat agctcctcgg tgacgaaggg
     1681 catgtaaggg tgcagcaggc gcaggatgcc gtccagcacc gttcgggcca ccgccagcgt
     1741 agcgccccgc gtctcggcgt cctcgctgcg caacgctccc ttggccgcct cgatatacca
     1801 gtcgcagtac tcgctccaca cgaactcctg caacaccttg cccgcctccc caagctggta
     1861 gtcctccagc atctgcgtca cctgcgacac tgtgcggctg taccggctca gaatccatct
     1921 atcggcgagg gattttggat tttggatttt ggattttgga ttgttgtcgt ccatcgtctg
     1981 tgcaagcacg aaccttgtcg cgttccagag cttgttggcg aagtggccgg cttgctcggt
     2041 gcgctggtcg ctgaacttct ggtcgttgcc gggggaggag ttggtgatga gggagaggcg
     2101 cagggcgtcg gagccgtagc ggtcggccag gccgatgggg tccaccacgt tgccccagga
     2161 cttgctcatc ttgcgccccg tctcgtccct gacaaggccg tgcaggtaca ccgtgtggaa
     2221 gggtatcctg ccggtgtgct ctatcccctg gaagatcatg cgggccaccc agaagaagag
     2281 tatgtcgtag ccggtctcca tcacgtcggt cgggtagaag tacttgaagt cctcggtctc
     2341 ctccggccag ccgagcgtgc tgaagggcca caggccgctg gagaaccagg tatcgagcac
     2401 gtcctcgtcc tggcgcaaat ccgtgctgcc gcagatttcg cacgcggccg ccttgtcctc
     2461 tatggccgcg aactggtggc cgttgtcgca gtaccacacg gggatgcggt gcccccacca
     2521 tagctggcgg gagatgggcc aatcgcggat gttttgcatc cagtggtagt acaccttcgt
     2581 gaaacgctcc ggcacgatgc ggatttctcc ccggcgcacc acctcgattg cgggggcggc
     2641 gaggggctgc atcttcacga accactggtc cgacacgata ggctcgagca ccgtgccgca
     2701 ccgctcgcag tggggcacgc tgtgggtgtg cgcgtctatc ctgatgaggt agccctcgtc
     2761 ctcaagctgc ttccccacgc ccgcgcgggc ctcctccacc cccagcccgg cgtacaagcc
     2821 cgcgttgggg ttgagcgtgc cgtccaggtt gaggaggttg atgatgggca ggtcgtgacg
     2881 ctggcctatc tcgaagtcga gggggtcgtg gccgggggta atcttcaccg cgccggtgcc
     2941 gaactccttg tccacaccag tgtcggctat caccgggatg gggcgctgca tgatgggcaa
     3001 gagcacctct ttgccgatga ggtgctgcca gcgctcgtcg tccgggtgca cggccacggc
     3061 agtgtcgccc agcatcgttt cggggcgcgt ggtggctact tcgatgccct ggctggggtc
     3121 gcccgcgagg gggtagcgta tcgtccagag gtggccttgc tcctcctccg gctccacttc
     3181 gaggtcggag agggtggtca tgcagcgggg gcaccagttc acgatgcgct cgcccttgta
     3241 gatgaggccc ttgtcgtata ggcgcttgaa cacggtgcgc acggcgctca cggggccggg
     3301 gtccagtgtg aagcgctcgc gggtccagtc gcaggaggcc cccaggcggc gaagctggta
     3361 gcgtatgcgg gggcggtact cgttcatcca ctcccaggcg cgttccagga acttctcgcg
     3421 gcctatgtcg tgccgggaaa gctcctcctt ggcaagctcc ttctccacca cgtactgccc
     3481 cgatataccg gcgtggtcct cccccggcag gtagagggcc tcgtagccct gcatgcgccg
     3541 ccagcggatg aggatgtcct ccaccgcgtt ggtgagggcg tgccccatgt gcagttcgcc
     3601 ggtcacgttg ggcgggggca tgatgatggt gtatggctta cgattggggt cgcgcgaggc
     3661 ggtgaagtag ccctgcccct cccaccagtc gtaccagcgt ctctccaccg cctcgtggct
     3721 gtaggctttc gccatctcgt cgccctgttt ggcgctgtcc tctgctagtg ttgtgtctga
     3781 cattgtactt tcctccttag ccgggttctt atgctaatgc tctttgcgag acctataaaa
     3841 gataaagatg gaggggagag aactggggga cacccccagc ccccgtcagg ggagtacccc
     3901 tgcaccccct tgtgagggag ggaaccgggg gacaccccca gcctcccgtc aggggagtac
     3961 ccctgcaccc accgtgggaa ggaccgagga caacacaggc ccgtcagggg agtacccctg
     4021 cacccctttt cacgtgccga ccggggcgaa atcgggcagc acgtccagga cgctggcgca
     4081 tagctcgtag cggccgaagg agggcatgag gtaggccccg gcgtagcggt tgtaggcgta
     4141 cttgaggatt tcctgggcca tctcgacgcc ggcttgcctg ccgttcgcgc ccgcttcttc
     4201 catgcgcttg agcacgaagt cggggatgac gatgccgggc acctcgttgt gcaggaactc
     4261 ggcatggcgg aagctttgca gaggcagtat gcccaggagc acgcgggcgt tgaagctttg
     4321 cacgctctcc aacgtgcggg ccacgaggtc gaggtcgtac acgggctggg tcatgacgaa
     4381 gtgcgcgccc gcttcgagct tctggccgag gcggtctatt tcgagcggga ggtcgcgggc
     4441 ggtggggttg aaggcgcagc ctatcaggaa gtcggtggtg ccgccgatgc tgttatccgc
     4501 caggtcgagg ccgtcgttga ggcgggtcag cagcttgatg agaccgatag cgtcgatgtc
     4561 gaacacggcg gtggagccaa ggcggtccac ggggttgggg gcatcgcctg tgagggcgag
     4621 tatgtggcgc aggcccatcg cgtgggcacc gagtagctcg gcctgtatgc ccatgagcgt
     4681 gcggtcgcgc gtggtgaagt gcacgatgac gcccatgttg acctgctgct gcacaaggta
     4741 gctcacggcc agggcgctca tggaggcgcg acccagggcg gagtcagtga cgtttaccgc
     4801 gtccacgccc ttggccttca gcatgtgagc gcccgccagc agcttggagg ggttcactcc
     4861 cctgggcggg gcaagctcga tgctgaccac gaactccttg cccagcttcc agccgaacga
     4921 ccccacctcc gtgtggaccg aagcgctggt gtcgctcagc tcaagggggg cggcttcgcg
     4981 gagcatgggg ggttcgggtt cggagacgcg caccggctga gccggctcct gggtggcggc
     5041 accgtccgcg acgccggtag cctcaagctc tgccacggtg gcggcgatgg cctggatgtg
     5101 ggcgggggtg gtgccgcagc aaccgccgag gaggcgcgcg cccgccgcca tcatacggga
     5161 ggcgaagctt gctacgtagt cgggggtgga cacgtaaacg atgcggtcgc ccaccatttc
     5221 cggccagccc gcgttgggct gggcggacag aggcgtcttg accacggcgg acatctcgga
     5281 gatgacgcgc gacatgcgct gggggccggt ggagcagttc gcgcctatga catcggcgcc
     5341 aagctcctcc aaggccctcg ctacttcggc cggggtgttg cccatctggg tgcggccgtc
     5401 gccgtcgaag gtcatctggg ccacgacggg caggtcgcag gcgtcgtggg cggcgatgag
     5461 ggcctggcgc atctcctcaa ggtcggcgat agtctccagg atgatgaggt ctacgccgcc
     5521 ttctagcagc gcctctatct gctcgcgaaa gacagtgcgg gcctccaaag ctggaatgag
     5581 gcccactggc ttgaggggct tgccgagcgg cccgaccgca cctgccacca tgacaggctg
     5641 cccgctgacc tcgcgcacgt cacgggccag gcgcgccccg cgcttgttga tctggcctac
     5701 cttgtcggcc aggtcgtagc ccgcgagctt gtgccggttg gcgccgtagg tgttggtctc
     5761 gatgatctcc gcacccgctt tgatgtaggt ttcgtgcact ttgcggacga ggtcgggctg
     5821 ggtgaggttg ctttcatcga agcagctatt ggcaggcacg cccatagagt agagcatggt
     5881 gcccatagcc ccatcgcaca cgattggccg cgcctcgcgc agccgcacga ggaaggggtg
     5941 attgctcttc accgatgtag ttccgttgcc tgccatgtca aactccagta gtgagtgccg
     6001 agttccgagt aatttaggtg cccactcggc actcggtact cggtgctcgg cacttaaaca
     6061 aaaacgcctc tacgcggaga ggccacaatt gccaggtatg aggctggccg aatccttccc
     6121 cttggggctg ggtggggcac cgcgcggttt gccggttgcc gggtaggtct gcgagccagt
     6181 tctctctcta ccactctcga tttctatttg gtttgatttg agtgtacacc aggcagggcg
     6241 aggtgtcaag ttgcgggcgc atatgggggc tggccttgct ttggccggtg gcaactacct
     6301 ggcaatgtgc cgtgaccaaa gccgagggtt tgttatcctg agtgggcctt gtcatcctga
     6361 gcgtagcgaa ggatcaggtg ggtaggcgag agtctacaac gctggagatg atgcaggcag
     6421 taggaagagt caagcacggg cggctgatcc ttcgctacgc tcaggatgac aggacggagc
     6481 aggagggtcg cgaagggtac cccccctgct tcgatggcac ctgatagcta cctgcccagc
     6541 ggctttgttt tggcgaggcc cacgcggcga gggtagccgg gcggggtgga ggcgactttg
     6601 cgaaccgaaa tgacggtgcg cggctcttgg tcaccgggga gggtgaaggg gtaggtcttg
     6661 ccgaggcggc cacctagcag cttggcggcc tgcttgcctt cggatagctc ctgggatatg
     6721 tctcccttct tgggggcgag taggtggccc cccactttgg caagggggag gcagtactcg
     6781 cagaggacgg gcagggcgga cacggcgcgg gcggtgacga ggtcgtagcg ctggcggtgg
     6841 agcttgtcct ggcctagctc ctcggccctg gcggtgacca cccgtacgtc ctctagggcg
     6901 acagcgcggg ctagctcctg caggaaggag gtcttcttgc ctacagagtc gttaagggtg
     6961 aggtcgatat ggggccagag tatcttgagg ggcacgcctg ggaagcccgc gccggtgccg
     7021 atgtcgatca ggcggaaggc ggcaggtggg gctgcgaggc cgggctgggt tccacgcagt
     7081 tccgagagga gggcggcggc tacggtgagg ctgtcgagga agtggcgcac ctgcacgagg
     7141 tcgtagtccg tgatggcggt gaggttgatg cggctgttcc agtctaccag ggtgtcgtag
     7201 taaagttgga actggtcaag ctggcggggg gtgagggtga agccgagctg gcgggcaccc
     7261 tcctgcagaa tggcaaggcg ggtctccacg ggctagatat accctcgctc gcgcagatgg
     7321 ccttcgcaca ggagcagggc cagggccgcg ttgccgtcct ggatgcgacc gtcgcggcac
     7381 atggcaaggg cttcggcggc gggcacgggc ctcacttcga tttgctcggt ctcttcggtg
     7441 gactgctcgc gttcgagggc cacgtccagg gcgaggtaga tgtggtacgc ctggtcgctc
     7501 tggcctaccg tggtgtagta gcagccgatg tagagcattt cgccacaggt cgcgcctatc
     7561 tcctgctcca gttccttgcc ggccacctcc tcgtgcgtgg caccgtccgc gtcgtgcatg
     7621 cccccggcgg gcacctccag gctccaggcg tcgatggtgt agcggtactg gcggatgagc
     7681 acgacctcgc cctgcttggt gaccggcacc acgcctaccg cgccggggtg ctcctggtag
     7741 ttgaactcga taggctcgtc cttgcctcgc agtcgcacgc ggtcctggcg tagggtaatc
     7801 cagggggaga cgaaggggta cgtggtgccg agcaggtgcc agccaagctg ctcgcccagc
     7861 ggcttaccct gcttttgcag tatgtggcta tcgccgttac cgctcatgca cgctccatca
     7921 gctcgattct attgccgaac gggtccgaca cgtagaagcg cgagacgccg gggataggtt
     7981 cggcttcagc aatgacgatg ccagcctctt cgagcgagcg ccgcagagcg ggcaggtcgc
     8041 gcacgtggag ggccgggtgg gctttgaggg cggggctgaa ttggtcttcg aggccgacgt
     8101 gtagctctcc agcgccgaga gagaaccaga cgccgccttt cggcttgagg atgtcgggct
     8161 tctctagctc ccggaggccc agcagaccgg cgtagaagtc acgggcctgc tgctcgacgc
     8221 cctcgcgcct gggagcggcc acctggacgt ggtcgaggcc aattaccggc acgtaagtta
     8281 ccctttctcc acgaggtgtg gggtgaagcg cagggcctcg aggtcggcgg cccctatgcc
     8341 gaacatggca atgcgtagct ggtcgcgcag cactccgcct agctccacgg tggcctcggc
     8401 ggatatggcg gcggctttga ggaaggggcg ggcgatacct acgaggtcgg caccgagagc
     8461 gagggctttg gcggcgtcta tgccgttgcg tatgccgccg ctggcgaaca ccttgccacc
     8521 ctggggcagg ttgagcgtga gcgctttgag gcagtcggcg gtggggatgc cccagtcggc
     8581 gaaggcctca gccaccaggc ggtcgcgctc ggcggggatg cggtggagtt ccacctggct
     8641 ccaggaggtg ccgcccgcgc ccgcggcatc gatggccgag acgcccgcct cccacaggag
     8701 ggtggcggcg tcctcggaga tgccccagcc cacctccttg acgattaccg gcacgtcaag
     8761 cccccggcag acctgggcga ttttagcggc caacccggcg aaattggtgt cgccttcggg
     8821 ctgcacggcc tcctggatgg cgttgaggtg gaggatgagg gcgtcggcct ctatcatgtc
     8881 cacggcgcgc ttgcactcgt ccagggtata ggcgtagttg agctggacgg cgccgaggtt
     8941 ggcgaagagg ggcacgtcgg gggcgtagcg gcgcacctgg taggtggggg cgagctgtgg
     9001 gtctttgagc gcggcccgct gcgagccgac gcccatcgcg aggccaagct cctgcgccgc
     9061 gattgcgagg ttgcggttga tctcctcggc cctgggcgcg ccgccggtca tggaggagat
     9121 gaggaggggg aggcgcaggg tgtggagcca gagccggagg gaggtgtcca ccgcggcgag
     9181 gtcaagctcc ggcagggcct ggtgcacgaa cctgtagcgt tcgaggcccg tggtgatgcc
     9241 tcgcccgctg acgtcctctt ccaggcagat gcgaatatgg tcggctttgc ggttctcaat
     9301 cgacatggct attgcaggtg cattgtactg tgcttccagc atgggtgaca acgtgaaatg
     9361 taagagacga aaacgcaaag acgcaaagga cgcaaagaac gcgcaaagag aagaatagtt
     9421 tagtgagtaa atagaggacc agagtacatt tatcgtaccc ttgccccctt tgcttagaac
     9481 aacacaatcc taatctctgc gcgtgctttg cgtcctttgc atcccttcgg ctgcgctcag
     9541 ggcatgcttg gcgttttcgt ctcttgtcca tactggcaac agggtggcta tttgacgact
     9601 tcgaggacga agaggagttc ctggcctggg atgatgtagg cgaggtgttc ttcgtataag
     9661 gggaggaagc ggtttatg
//
LOCUS       DASRWZ010000675         5466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1014079, whole genome shotgun sequence.
ACCESSION   DASRWZ010000675 DASRWZ010000000
VERSION     DASRWZ010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014079"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..2111
                     /locus_tag="VFH60_10690"
     CDS             90..2111
                     /locus_tag="VFH60_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HET6314296.1"
                     /translation="MEDVRPGDGVGGTGEEGSGEPTQEQVTRREFVRRAALALGGAVA
                     AGAVFSTLGYDTADAVVDTDAVVGKGPTAAEPGTSASARLHNNTSLAQPAATAPTARP
                     TGTRSNAPASQPAATKAAAPTGTPTPQPVPPAGTLPPMDLSSNPYSRQRQATIARNGV
                     VATSTPLAVEAGLLMLRKGGNAVDAALAAAIAQTVVEPTSNGIGSDAFAMVWDGKKLH
                     GLNGSGRAPRSLTLDLLRRKGYTSIPYRGWSSVTVPGAPALWRDLHARFGKLPFDTLF
                     EPAIEYAERGFRVSPVVAYHWGRSVPIYSPNKGPEYKGWLETYAPGGRAPRAGEVWSS
                     KALGTSLRRIAQSKGDDFYKGELARTMARFSAQTGGFLAEADLAAHTSTWVDPISTTY
                     RGYEVWEMPPNGQGITALIGLNILEGFELDKLARDSTRSFHLQIEALKLAFADAFRYV
                     ADPDFVPVPVAGLLDKGYAATRRALIGNRALQPAPGKPNSGGTVYLCAADADGMMVSF
                     IQSSYMGFGSGIVVPGTGIALHDRGANFSLDPNHPNRLAPGKRPYHTIIPGFLTRNGQ
                     AVGPFGVMGGFMQPQGHIQVITGTLDHHLNPQAILDAPRWQWTSGKSVQVESDADPAI
                     IKALRGAGHQVSVLQPSGTFGRGQIIWRLPQGGYAAGSDKRADGHAGGM"
     gene            complement(2196..3170)
                     /locus_tag="VFH60_10695"
     CDS             complement(2196..3170)
                     /locus_tag="VFH60_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6314297.1"
                     /translation="MREDPTSRPQENIISRWRNRLAAAMLNRQGRALHDKGEYQQAIE
                     EFTRAIELSPDLAESYNRRGSAYAALGDHDRAIADYNKAIAYSPDMPQPYLDRATSLH
                     AKGDLERAIADLTRAIRIKPDFLPAYTQRIAIYRSLGDEDLAQADEDQLKKLSPTRAI
                     EEYSRLIETDPNDPVLYHNRGSAYGIKQAYDRALDDFNKAIALDPDYAQAYDNRGVIY
                     AEKKLYDSAIEDFDRAVELRPDYPEAYNNRGTAFGEIGNLDQAVRDFTRAIDLRRDYL
                     EAYINRATAYRVKGAKEAAAADLRHVRDVTPNPSMRREMEKRLKELGE"
     gene            3362..3853
                     /locus_tag="VFH60_10700"
     CDS             3362..3853
                     /locus_tag="VFH60_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6314298.1"
                     /translation="MKILLLLRHGKAQESAPGGDKARRLTGQGRLEAAEVGSRLSRIA
                     GRLDDVLSSDAARALDTARIAASGAGYSGEVRVEPDIYSADVDTLLDVLRGMRAGECV
                     LLVGHNPGLEYLAAALAQEGTLEPSLPTGGLVHLTFEADRWRDVRPHTGRLLGVYRPG
                     SDE"
     gene            complement(4063..4701)
                     /locus_tag="VFH60_10705"
     CDS             complement(4063..4701)
                     /locus_tag="VFH60_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET6314299.1"
                     /translation="METEAKFVVPDEATFSCLRQLTAVGPYAVRDTRTKHTHDRYLDT
                     EDRSFFSRNLYVRLRQLSGGVLLLTVKGLGLGVREGSIHTRDEYESQVSGLDVAGWPQ
                     GDVRRIVEETAQGKPLHDLYSLDQIRTVSNLYQGDRAAAELSVDEVAFHTATGPVRAY
                     ELELELLPHGQLSDLRELSHLLTEEYHLLPQPLSKFERAMLLNMERRVDGDE"
     gene            complement(4747..>5466)
                     /locus_tag="VFH60_10710"
     CDS             complement(4747..>5466)
                     /locus_tag="VFH60_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6314300.1"
                     /translation="ILQEDGIDVLFEAESLRVARGGDNKVLFTVHAPGGERVLEGSHL
                     LVATGRTPNSDSLNLGAAGIETDERGFIKVNGKLETNVPGVYALGEANGEPAFTHISY
                     DDFRVICANLLYGQDVTTDDRILPYVVFTDPQLGRVGMNEEDARKQGRNVKVARMPMT
                     WVARALETGETRGLMKAVVDADTGQILGCAVLGLEGGEVMAQVQLAMMGKLPYHVLRD
                     AIFAHPTLAESLNTLFAQVEE"
BASE COUNT          895 a         1699 c         1872 g         1000 t
ORIGIN      
        1 cgcgtagagg gtctcttgca ggacgtaggg gatgccctcc atgtgttata gtggactgcc
       61 ttgcacgagt gaggcaggaa ggaacgggta tggaggacgt gcgccccggt gatggcgtag
      121 gcggcaccgg agaggaagga agtggggaac ccacgcaaga gcaggtcaca aggcgcgagt
      181 tcgtgcggcg ggcggccctg gcgttgggcg gggcggtggc ggcgggagct gtcttctcca
      241 cgctggggta tgacacggcg gacgcggtgg tcgatactga tgccgtagtt ggtaaaggcc
      301 caaccgctgc cgagcctggt acttcggcct ccgcacgcct gcataacaac acctctctcg
      361 cgcagccggc agccaccgcc cctactgcca ggcccaccgg cacccgctcg aacgctcccg
      421 caagccagcc ggcggccacc aaagccgccg cacctaccgg cactcccacg ccccagccgg
      481 tcccacctgc cggtacgctg cccccaatgg acctatcgag caacccctac agccgccagc
      541 gtcaggcgac cattgccagg aacggggtcg tcgctaccag cacgccgctg gccgtggagg
      601 ccgggttgct catgctgcgc aagggcggca acgcggtaga cgccgcgctc gcggccgcca
      661 tcgcgcaaac ggtggtggaa ccgacctcca acggtatcgg ctcggatgcg ttcgctatgg
      721 tgtgggacgg caagaagctg cacgggctga acgggtcggg ccgcgctccc aggtcgctca
      781 ccctcgacct gctcaggcgc aagggctaca ccagcatccc gtacaggggt tggtcctccg
      841 tgacggtgcc cggcgcgccc gctttatggc gcgacctcca cgctcgcttc ggcaagctgc
      901 ccttcgacac gctcttcgag cctgcgatag agtacgccga gcgaggcttc agggtctcgc
      961 ccgtggtggc ctatcactgg ggccggtcgg tgcctatcta ctccccgaac aagggaccag
     1021 agtacaaggg ctggctggag acttacgcgc cgggcggacg cgccccgagg gcgggcgaag
     1081 tgtggtccag caaggcgctg ggcacgagct tgcggcgcat cgctcaatcg aagggggacg
     1141 acttctacaa gggggagcta gcgcgcacga tggcccggtt ctcggcgcaa actggcggct
     1201 tcctggccga ggcggacctg gcggcgcaca cgagcacctg ggtggaccct atctcaacca
     1261 cctacagagg ctacgaggtg tgggagatgc cgcccaacgg gcagggtatt acggccctaa
     1321 tcggcctcaa catcctggaa ggcttcgagc ttgacaagct ggcgcgggac agcacccggt
     1381 cgttccacct gcaaatagag gcgctcaagc tggcctttgc cgacgccttc cgctacgttg
     1441 ccgaccccga tttcgtgccg gtgccggtcg cgggcctgct cgacaagggc tacgcggcga
     1501 cccggcgcgc cctcataggc aaccgcgccc tccaacccgc gccgggcaaa cccaattcgg
     1561 gcggcacggt gtacctgtgc gcggcggacg cggacggcat gatggtcagc ttcatccagt
     1621 cgagctacat gggcttcggg tcgggtatcg tggtgccggg cacgggcatc gcgttgcacg
     1681 accggggcgc gaacttcagc ctggacccca accacccgaa ccgcctcgcg ccgggcaagc
     1741 gcccatacca caccatcatc cccggcttcc tcacgcgcaa cggccaggcg gtcggcccct
     1801 tcggcgtgat gggcggcttc atgcagcccc agggtcacat ccaggtgata accggcacgc
     1861 tggaccatca cctcaacccc caggccatcc tggacgcgcc ccgctggcag tggaccagcg
     1921 gcaagagcgt gcaggtcgag tcggacgccg accccgccat aatcaaagcc ttgaggggag
     1981 cgggccacca ggtaagcgtg ctacagccga gcggcacctt cggtcgcggc cagattatct
     2041 ggcggctccc ccagggcggc tacgcagcag gcagcgacaa acgagccgac ggccacgccg
     2101 gagggatgtg acgatttgga aattggaatt tcggatttcg aatttagtag atatccagca
     2161 aattcgaaat ccgaaattcc aatttccaat tttcttcact ctcccagctc tttgagccgt
     2221 ttctccatct cgcgccgcat gctggggttg ggggttacgt cgcgtacgtg gcgcaggtcg
     2281 gcggcggcgg cctctttcgc gcccttcacg cggtaggcgg tggcgcggtt gatgtaggct
     2341 tccaggtagt cgcgccggag gtcgatggcg cgtgtgaagt ctcggacggc ctggtcgagg
     2401 ttgccaatct cgccaaaggc ggtgccccgg ttgttgtaag cttcggggta gtcggggcgt
     2461 agctccaccg cgcggtcgaa gtcctcgatt gcgctgtcgt acagcttctt ctcggcgtag
     2521 atgacgcccc ggttgtcgta ggcctgtgcg taatcggggt caagcgcgat agccttgttg
     2581 aagtcgtcca gggcgcggtc gtaggcttgc ttgatgccgt atgccgagcc gcgattgtgg
     2641 tagaggacgg ggtcgttggg gtcggtctct atcagccggg agtactcctc aatagcgcgg
     2701 gtagggctga gcttcttaag ctggtcctcg tcggcctggg caaggtcttc atctcccagg
     2761 ctacgataaa tggcgatgcg ctgggtatag gcgggcagga agtcgggctt tatgcggatg
     2821 gcgcgggtga gatcggcaat ggccctttcg aggtcgccct tcgcgtgcag ggaagttgcg
     2881 cggtcgaggt aaggctgggg catgtcgggg ctgtaggcga tagccttatt gtagtcggca
     2941 atcgcccggt cgtggtcgcc cagggccgcg taggcactgc cccgcctgtt gtagctctcg
     3001 gcgaggtcgg ggctaagctc gatagcgcgg gtgaactcct caatggcctg ctggtactcc
     3061 cccttgtcgt gcagcgcgcg gccctggcgg ttgagcatgg cggctgccag gcggttgcgc
     3121 cagcgagaga tgatattttc ctgtgggcgg ctcgtggggt cttcgcgcaa gggtgccttc
     3181 cggtctggtg gagtgtgtgg ggtgaacttc gcggaattat agcacagggc aacgtgcgtc
     3241 acctagtgcc cgttgtttcg cctcggtgcc gtagcagcag ccggtatgag ggccaactca
     3301 gccgtgggcg tggtatgcgg tattatagct acatgacacc aaccggcagc ggagggacgc
     3361 attgaagata ttgctgttgc tgcgacacgg caaggcgcaa gagagcgcac caggtggcga
     3421 caaggcgcgc cgcctcaccg ggcagggcag gttggaggcg gcagaggtag gcagcaggct
     3481 gtcgcgcatc gccggcaggc tggacgatgt gcttagctcg gacgcggcac gcgcgctgga
     3541 cacggcccgt atcgcggcgt cgggtgcggg ctatagcggc gaggtgcgcg ttgagcctga
     3601 catctacagc gccgatgtgg ataccctgct tgacgtgttg cgtggcatga gggccgggga
     3661 gtgcgtgctg ctggtggggc acaatcctgg cctcgaatac ctggccgccg cgctcgccca
     3721 ggaaggcacg ctggagccgt cgctgcccac cggcgggctg gtccacctca ccttcgaggc
     3781 ggaccgctgg cgcgacgttc gcccgcacac aggcaggctg ctgggtgtat acagaccggg
     3841 gagtgacgag tgactagttg tcgcaaacat tgcaggtgac gtcccgctat agccctgtgg
     3901 gacgccctgc caatgaattg gcgggcttac aagaggcaaa gtctcctacg gagactaagg
     3961 agcatgtagt agtctccgta ggagactttg cctcttgtaa gcccgtggct tcagccacag
     4021 ggctatctct gggcgaccat gcaggacgtc atattggagt cgtcactcgt caccatccac
     4081 tcgtctctcc atgttcaaca gcatggcccg ctcgaacttg gagagcggtt gaggcaggag
     4141 gtggtactct tccgtgagga ggtggcttag ctcgcgcagg tcggagagct ggccgtgcgg
     4201 gagcagttcc agctctagct cgtaggcgcg gaccggcccg gtggcggtgt ggaaggcgac
     4261 ctcgtccacg ctcaactcgg ctgcggcgcg gtcgccctgg tagaggttgg agacggtgcg
     4321 gatctggtcc aggctgtaga ggtcgtgcag gggcttgccc tgcgcggtct cctcgactat
     4381 ccggcgcacg tcgccctgcg gccaaccggc aacgtccagg ccggacacct ggctctcata
     4441 ttcgtcccgc gtgtgtatgc tgccctcccg cacaccgagg cccagccctt ttaccgtgag
     4501 cagcaggacg cctcctgaga gttggcgcag ccggacatag aggttgcggc tgaagaagct
     4561 gcggtcctcg gtgtccaggt agcggtcgtg ggtgtgtttg gtgcgggtgt cgcggacggc
     4621 gtaggggccg accgccgtca gttggcgcaa gcagctaaag gtggcttcgt ccggcaccac
     4681 aaacttggct tcggtctcca tgttggtatc tccagcggcg cggcgctttg tccccactac
     4741 accgcgttac tcctctacct gggcgaagag cgtattgagg gactcggcca gggtggggtg
     4801 ggcgaatatg gcgtcgcgca ggacgtggta ggggagcttg cccatcatgg ctagctgcac
     4861 ctgggccatg acctcgccgc cttccaggcc gagcaccgcg cacccgagta tctgcccggt
     4921 gtccgcgtcc accacggctt tcatcaggcc gcgcgtctcg ccggtctcca gggcgcgcgc
     4981 tacccaggtc atcggcatcc tggccacttt gacgttgcgg ccctgcttgc gggcgtcctc
     5041 ctcgttcatg ccgacccggc ctagctgggg gtccgtgaac acgacgtagg gcaggatgcg
     5101 gtcgtccgtc gtaacgtctt gcccgtagag gaggttggcg cagataacgc ggaagtcgtc
     5161 gtaggagatg tgggtgaagg caggctctcc gttggcctcg cccagcgcgt acacgcccgg
     5221 cacgttggtc tccagcttgc cgttcacctt gatgaagccg cgctcgtccg tttcgatgcc
     5281 cgccgcgccc aaattgaggc tgtcgctgtt gggggtgcgg ccggtggcta ccagcaggtg
     5341 agagccttcc agcacgcgct ctccacccgg cgcgtgcacg gtgaagagga ccttgttgtc
     5401 gccgccccgc gccaccctta gcgactcggc ctcgaaaagc acatctatac cgtcttcttg
     5461 caggat
//
LOCUS       DASRWZ010000676         5721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_671993, whole genome shotgun sequence.
ACCESSION   DASRWZ010000676 DASRWZ010000000
VERSION     DASRWZ010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671993"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFH60_10715"
     CDS             <1..457
                     /locus_tag="VFH60_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HET6314301.1"
                     /translation="ADCPPVYFSDPVRRAIGLAHSGWKGTVARSSASVVQGMGRHFGS
                     RPEDIVAVVGPGIGPCCYKVGPSVVEAVESAYSRPWSVRLPLLELYEGDVYFNLWEGI
                     RRALVEAGVPPRNVAIDGLCTMHNTGIFYSHRGEAGKCGLFGAVLGIKE"
     gene            495..1226
                     /locus_tag="VFH60_10720"
     CDS             495..1226
                     /locus_tag="VFH60_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013333868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET6314302.1"
                     /translation="MSSEIADNVARVRERLARAAERVGRPVEDVTLVAVSKAQPVDRI
                     LQAVEAGVSVFGENRVQEAVAKFAGREGGDDGSMVLRHGIALHMIGTLQRNKARHAAR
                     HFDCIHSVDGGSLARDLDVAAGRERGGEPLSVLLEVNVTEEGNKSGVSVEELPRLAEV
                     LESCRNLRCNGLMTIARFGAGETELRETFARLRELLEQMRKSYPGDWRHLSMGMSHDF
                     EHAIEEGATMVRVGRAIFGERTRLG"
     gene            1226..2617
                     /locus_tag="VFH60_10725"
     CDS             1226..2617
                     /locus_tag="VFH60_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET6314303.1"
                     /translation="MYCLFPSLLTEADNFLDRLSQSARRQGYSLFLVGGSVRDLLMTR
                     PLHDLDLTTDAPVEGIKMLLEEAEADVVYGMGEKFGTIAARVGEITVEVTTFRNRDEA
                     VPGDLLSALHTDLAHRDFTINAMARDLATGELHDPFGGELDIKQQLVRAVGDPEARFA
                     EDPLRLLRAVRFAANFNFFIERETAEAIRRLAHLLEDVSRERVGDEMDRLLMSDPPSE
                     GISLLDNLGLLEYTVPELLEMHEMERGPLHYKEVYPHTLKVLDRTAPDLILRWAALLH
                     DIAKPQTYGITDGEVHFFGHEKVGAQMARRILSRLRRPHEVVEQVEQLVAEHLRIGLY
                     DEGWSDGAVRRFLRETAPINERLFALSRADITSQRAFRVAAALSRIDALYERCEQIKA
                     QEEVEKIASPLDGNELMQLFGGRPGRWIGEVKDYLLGLVLDGALAQDDKAEAERLARE
                     YVAEHQEILHGAV"
     gene            complement(2628..>5721)
                     /locus_tag="VFH60_10730"
     CDS             complement(2628..>5721)
                     /locus_tag="VFH60_10730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6314304.1"
                     /translation="AHHPGPVSTGLAPEQHIERDVQAVPGGATRGIEAWIPEARRPVE
                     ARPVERAKPVEKRAPRDMVRGAWKARPVWLGVLAFLVGAAGQVLLFEEPQRALGVVLL
                     VGALVLAMVAWSGLRDAPLLTAAPEPARRLLNMRPELALRLVGIAVSLGLLVGSYLAW
                     YRQPDDIFGWQGVLWLASMAVLLISCAQWYPGSKRQDPLETPWPRWEYAVLAALVVVS
                     LVTHLTWLDRYPYEIDVNEYFAWYESMAFYRDPPIVSMFTTVWLNLGLPSLWFWFQAQ
                     FLHLFGVNLVGLRFITGLVGALTIIPVYLLSRLVWGRTAALLSAVPVTFLIVILHHSR
                     QSSNNIATGLFWTFCFYFLLKGLRTRRPADFVWAGLWAGTSQYTYYGTRLLPYLLVAF
                     GAYLAIFHFRAFRERLGLLALTAVGFMVGFGPLVAYFTRNPEKWAGRGLSELIIPLEI
                     PTNWDQIANIWNKVWPEVWKNFLSLSVIPANDTFFFAPFLRPFEMVLLALAFGVLIYR
                     WRQPASFLVLLWAGSVAAVSSIINIPINPNPNFTHWAPAWPVFFLAYGLAPALWLAAL
                     RQTSRRLWQAGSVLVAAGIIVLAFANFRFYIVDYPARVLTDPVLRSIQAKYLMQVKPG
                     SVARFVSCCWFAFDTEFGRAFAADTPAGQFLNPSRDLPLVMEPPRDQVFIMPRTVDAY
                     LPVLQHYYPGGQIDKLTGNDGTYVASAYKVPADRITARHGVSATVSYNGNVVATGKVP
                     TVGSLPPGVPTDRYPLQATWSGAFYVEKAGPATLSLQGASGRVWVMGQQVAPGEELTL
                     DAGWVPFTVDARLAGRSEVRLLLGSGGAVAEIPAKHLWPSEPTGGLTAAIRLPASEGD
                     NSTKVISRVDPFVGAGARRTPKGWSQKGILSDEEYQRTFEQTLPLAPTLPEAKDGDIW
                     VRWSGEVLSDEGTYSMELRTDGRAHLAIDGVPVVSKCEPTYTEGRAAADFELTAGWHR
                     VQLDLHVTPDQQRGLEWLWRRPDGVTEVVPPSRLRYATSTTPQALVQWPEPPTEIPCP
                     "
BASE COUNT         1077 a         1748 c         1917 g          979 t
ORIGIN      
        1 cgccgactgc ccgccggtct acttctccga ccccgtgcgg agggcgattg ggctggcgca
       61 ctcaggctgg aagggcaccg tggcccgcag ctcggcaagc gtggtgcagg gtatgggcag
      121 gcacttcggc tcgcggcctg aggacattgt ggcggtagtg gggccgggta tcgggccgtg
      181 ttgctacaag gtcggaccga gcgtggtgga ggcggtcgag agtgcgtatt cgcggccgtg
      241 gtcggtgcgt ttgccgctgc tcgagcttta cgagggcgac gtctatttca acctctggga
      301 gggcatacgc cgcgccctgg tcgaggcagg agtgcccccg cgcaacgtcg ccatagacgg
      361 cctctgcacg atgcacaata ccggcatctt ctactcccac cggggcgagg caggcaagtg
      421 cgggcttttc ggcgcggtgc tggggatcaa agaataatta cgaattacga attacgaatt
      481 acgagttgtg aagcgtgagt agtgagattg cggacaatgt ggctcgcgtg cgggagaggc
      541 tggcacgagc cgctgagcgg gtggggcgtc cggtggagga cgttaccctg gtggctgtga
      601 gcaaggcgca gcctgtggac cggattctgc aagctgttga ggcgggagtg agcgtctttg
      661 gggagaaccg ggtgcaggag gcggtggcga agttcgcggg gcgagagggt ggggacgatg
      721 gcagtatggt gctccggcac ggcatcgccc tgcacatgat cggcacgttg cagcgcaaca
      781 aggcccggca tgcggcgcgg cacttcgatt gcatccattc ggtggacggc ggcagcctgg
      841 cgcgtgactt ggatgttgct gcggggcggg agagaggcgg ggagccgctt tccgtgctgc
      901 tggaagtgaa cgtcacggaa gagggcaaca agtcgggcgt gagcgttgag gagttgcccc
      961 ggcttgccga ggtcctggag agttgccgta acttgcgctg caatgggctg atgaccatcg
     1021 cccgcttcgg ggccggggag acagaattgc gtgagacgtt cgcgcggttg cgagagttgc
     1081 tcgaacagat gaggaagagc tatccgggcg actggcggca cctgagcatg ggtatgagcc
     1141 acgatttcga gcacgctatt gaggaaggcg cgacgatggt cagagtgggg cgcgccatat
     1201 ttggcgaaag aacgcggttg gggtgatgta ctgcttgttt ccgagcttgc tgactgaggc
     1261 cgacaacttt ctcgacaggc tgagccagag cgcgcggcgg cagggctaca gcctcttcct
     1321 cgtgggcggc agcgtgcgcg acctgctcat gacccgtccc ctccacgacc tcgacctcac
     1381 caccgacgcg cctgttgagg gcatcaagat gctgctcgaa gaggcggagg ccgatgtcgt
     1441 ctacggcatg ggtgagaagt tcggcaccat cgcagcccgc gtgggtgaaa tcacggtgga
     1501 agtgaccacc ttccgcaacc gcgacgaggc cgtacccggc gacctcctgt ccgccctcca
     1561 caccgacctg gcgcatcggg atttcaccat caacgccatg gcgcgggacc tggcgacggg
     1621 cgagttacac gaccccttcg gcggtgagct tgacataaaa caacagctcg ttagggccgt
     1681 gggcgacccg gaggcgcgct tcgctgagga ccccttgcgc ctgttgaggg ccgtgcgctt
     1741 cgccgccaac ttcaacttct tcatagagcg cgagacggcg gaggcgattc gcaggttagc
     1801 ccacttgctg gaagacgtgt cccgcgagcg ggtgggcgac gagatggacc ggctgctgat
     1861 gtccgacccg cccagcgagg gtatcagcct gctggacaac ctgggcctcc tcgaatacac
     1921 cgtgccggaa ctacttgaga tgcacgagat ggagcgcggg ccgctgcact acaaggaggt
     1981 ctacccgcac accctcaagg tgctggaccg caccgcgccc gacctgatat tgcgctgggc
     2041 ggccttgctg cacgacatcg ccaaacctca gacgtatggc atcacggacg gtgaggtaca
     2101 cttcttcggg cacgagaagg tgggcgctca gatggcacgc cgcatcctca gccgcctgcg
     2161 ccgcccccac gaggtagtcg agcaggtgga gcagcttgtg gccgagcacc tgcgcatcgg
     2221 cctgtacgac gaaggctggt ccgatggggc cgtgcgccgc ttcctgcgcg agaccgcccc
     2281 tatcaacgag cgtctgttcg ccctctctcg cgccgacatt acctcgcaac gcgccttccg
     2341 cgtagccgcc gctttgagcc gcatcgacgc cctctatgag cgatgcgagc agatcaaggc
     2401 gcaggaggaa gtcgagaaga tcgccagccc cctggatggc aacgagctaa tgcagctttt
     2461 cggcgggagg cccggccgct ggattggcga ggtgaaggac tacctgttgg ggctggtgct
     2521 cgatggcgcg ctggcgcagg acgacaaggc ggaagcggaa cggctggcgc gagagtacgt
     2581 cgcggagcac caggaaatcc tccacggcgc ggtgtgagtg gctacgccta cgggcagggg
     2641 atttcggtag gcggttcggg ccactggaca agcgcctgcg gggtcgtaga cgtggcgtag
     2701 cgcagacggc tgggaggcac cacctccgtc acgccgtccg gccgcctcca gagccactcc
     2761 aggcctctct gttggtccgg cgtgacgtgg aggtctagct gcacacggtg ccaacccgcc
     2821 gtcaactcga agtccgccgc tgctctgcct tcagtgtacg tcggctcgca cttgctcacg
     2881 acaggcacgc catctatggc aagatgcgcc ctgccatcgg tacgcaactc catactgtac
     2941 gtgccttcgt cgctgagtac ttctccgctc caccggaccc agatgtcccc gtccttcgct
     3001 tccggcagcg tcggcgcgag cggcagggtc tgctcaaaag tgcgctggta ttcttcatcg
     3061 ctcaggatgc ccttctggga ccagcccttc ggcgttcgtc gcgcgccagc acccacaaag
     3121 ggatccaccc ggctgatgac tttagttgag ttatcaccct cagaagcagg tagcctgatg
     3181 gcggcggtca gtcccccggt tggttccgaa ggccagaggt gttttgccgg aatctccgcc
     3241 accgccccgc ccgacccaag cagcaaccgg acctcggacc ggcctgccag gcgggcgtct
     3301 accgtgaatg gtacccagcc cgcgtccagg gttagctcct cgccgggggc tacctgttgg
     3361 cccattaccc atacccggcc ggaagcccct tgcaggctca gtgtggccgg tcccgccttt
     3421 tctacataga atgccccgct ccacgtggcc tgaagcggat agcggtcggt ggggacgcct
     3481 gggggaagcg agccgacggt aggcaccttg cccgttgcga ctacgttgcc gttatagctg
     3541 accgtcgcgc ttacgccatg tcgggcggtg atccggtctg ccggcacttt gtacgcagac
     3601 gccacgtacg tgccgtcgtt gccggtaagc ttgtctatct ggccgccggg atagtagtgt
     3661 tgcagcacgg gcaggtaggc gtccaccgtc ctcggcatta tgaacacctg gtcgcgtggc
     3721 ggttccatca ccaggggcag gtcgcggctg gggttcagga attgacctgc cggagtgtcg
     3781 gcggcgaacg ctctgccaaa ctccgtgtcg aaggcaaacc agcagcagct gacaaagcgg
     3841 gcaacgctgc cgggttttac ctgcataagg tacttggcct ggatggagcg tagcaccggg
     3901 tcggtcaaga cgcgcgccgg gtaatcgact atgtagaagc ggaagttggc gaaggccagc
     3961 actatgatac ctgcggccac aaggacgctg ccggcctgcc acagccgcct ggaggtctgt
     4021 ctcagtgccg ccagccacag cgcgggtgcc agcccgtaag ccaggaagaa cacgggccag
     4081 gcgggtgccc agtgggtgaa gttaggattg gggttgattg gtatgtttat gatcgaggac
     4141 acggccgcga cgcttccggc ccataatagg accaggaacg aagcaggctg ccgccacctg
     4201 tagatgagca ctccaaacgc gagggccaac agcaccattt caaaggggcg cagaaaaggc
     4261 gcgaagaaga acgtgtcgtt ggccggtata acgctaaggc tcaggaagtt cttccatacc
     4321 tccggccaca ccttgttcca gatgttggca atctggtccc agttggtagg gatttcaagc
     4381 ggtattatta gctcggacaa gcctcttccg gcccactttt cgggattgcg cgtgaaatag
     4441 gccaccagcg gcccgaagcc caccatgaaa cccaccgccg taagggccag caggccaagg
     4501 cgctccctga aggctcggaa gtggaagatt gccaggtacg cgccgaaggc caccagcagg
     4561 tagggcagga ggcgcgtgcc atagtaggtg tactggctcg tacctgccca taggccggcc
     4621 cacacgaagt ccgcgggccg gcgggtgcgc aggcccttga gcaggaagta gaagcagaaa
     4681 gtccaaaaca gtcccgtggc gatgttattg gacgactgcc tgctgtggtg caggataaca
     4741 atcaggaagg tgaccggcac ggcggacaga agcgcggcgg tgcggcccca cacgagccgg
     4801 gacagcaaat agaccgggat aatggtgagc gcgccgacga ggccggttat gaagcgcagc
     4861 cccacgagat tcacgccgaa caggtgcagg aactgggcct ggaaccagaa ccacaggctc
     4921 ggcagcccca ggttcagcca caccgtggtg aacatggaga cgatgggcgg gtcgcggtag
     4981 aaggccatcg actcgtacca ggcaaagtac tcgtttacgt cgatctcgta cgggtacctg
     5041 tcgagccagg taagatgcgt aaccaggcta acaactacca gggcggccag cacggcatat
     5101 tcccaccggg gccagggcgt ttccagcggg tcttgccttt ttgagccagg gtaccactgg
     5161 gcgcacgata tgagcaggac tgccatgctc gccagccaca gcaccccttg ccagccgaat
     5221 atgtcatctg gctgcctgta ccaggccaag taagagccaa ccaggaggcc aagcgagacg
     5281 gcgataccca cgagcctcag cgccagttcg ggccgcatgt tcagtagacg acgggcaggc
     5341 tccggtgccg ccgtcagcag cggcgcatca cgcagtccgc tccacgcgac catcgccagc
     5401 accagcgcgc ctaccagcag cacaacgccc agcgcccgtt gcggctcctc gaacagcagc
     5461 acctgcccgg ccgcgcctac gaggaacgcc agcacgccta gccagaccgg ccgggccttc
     5521 caagctcctc tgaccatgtc gcgaggagca cgcttctcaa caggcttcgc gcgctcaacc
     5581 ggcctggctt cgaccggacg ccgcgcctca ggtatccagg cttcgatgcc gcgagtggca
     5641 ccgcccggta ctgcttgaac gtcgcgctca atgtgctgtt caggggctaa accggtactg
     5701 acgggcccgg ggtgatgcgc t
//
LOCUS       DASRWZ010000677         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_54787,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000677 DASRWZ010000000
VERSION     DASRWZ010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54787"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFH60_10735"
     CDS             complement(<1..512)
                     /locus_tag="VFH60_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314305.1"
                     /translation="MGISFGCGISLLVLVTGGIASLGPSGSQVPVPLETFSARLLLDI
                     VRFAVVPALAFAGTVTLCFHAIVRSVLPDNGVRRWAGLTFTSALGYMLLFAATFYLVL
                     PPVLGPLNSQLLLAGGVAQAGVVGLIALTGAFLGLVSGSALGVGQWASLRKYTQDARR
                     FSVAIAAGNAL"
     gene            complement(596..>2215)
                     /locus_tag="VFH60_10740"
     CDS             complement(596..>2215)
                     /locus_tag="VFH60_10740"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HET6314306.1"
                     /translation="MASQLTIDSPAKSAAQTGTATASAPRSPGIRIVPNLASPSRVLW
                     AGLGLGMAFDVLFYRKMLGISFPLFVASLLGVLFWLGRQEDVRPRMRNMWLVLPICFF
                     ASMVYFRVDGFLTFLNVCAVLGLLVLLAYFYAAGRLDRLGILGYYLSLMTAGLHSVTL
                     PFTLVPKSMEGRQLGGKSRDKWMPVLRGVMLAAPVLLVFAALLSSADMVFASLLGQML
                     EFRYLPDMGDLFTQVVWAVIVGWVLAGGLAFAVGRRDGASAGDEEPWDAGMDAVPTRV
                     SIGFVEVTTVLVLVNALFLVFGLVQFAYLFGGESNISAAGLTYAEYARRGFFELVAVS
                     VLTLGLILGLHHLTRRDTARQKLYFNALGSLMVALVLVLLASAYWRLSLYEQAYGYTH
                     LRLYVEVFELWLAATFMWLLLALWWKPKRFAIGAFVAALGFLATLNLINPDATIARGN
                     LERYHATGKLDASYLMGLSADAVPALVPVLDTMPPSPERSALVRDLWSRLRTFERSSE
                     WREWQALHWSRSEAFALLTANRDRIKQEDIR"
BASE COUNT          470 a          723 c          713 g          309 t
ORIGIN      
        1 agggcattgc ctgccgcgat tgcgaccgaa aagcgtctcg cgtcttgagt atacttccgc
       61 aagctcgccc actgaccgac acctaacgcg gagccactga ccagccccag gaacgcgccc
      121 gtgagggcaa tcaggcccac cacaccggcc tgcgccacgc cgccggccag tagtaattgg
      181 ctgttgaggg ggccaagaac agggggcagg accaggtaga atgtcgcagc gaacagtagc
      241 atgtaaccga gcgccgaggt gaaggtcagc cccgcccacc gccggacgcc gttatcgggg
      301 agaacagacc gcacaatggc gtggaagcat aacgtcacgg tgccggcaaa agctagcgcg
      361 ggcacgaccg cgaaacggac gatgtccaac aatagtcttg cagagaacgt ctccagtggc
      421 accggcacct gcgaccctga aggccccagt gacgcgatgc cgccggtcac caggaccagc
      481 agacttattc cgcagccaaa gcttatgccc atgacggcca tacacccgct accaactgcg
      541 agcgcactga gctcgtcacg ggctgcgttc atcttcacgg cggcggtgct gctctctatc
      601 taatgtcctc ttgctttatg cgatccctgt tggcggtcaa cagcgcgaac gcctcgctcc
      661 ggctccagtg cagcgcctgc cattcgcgcc attcggaact ccgctcgaac gtgcggaggc
      721 ggctccacag gtcgcggacc aacgcactgc gttccgggga gggcggcatg gtgtcgagca
      781 cgggcacgag ggcgggcacg gcgtccgcgc taagccccat gaggtaggag gcgtccagct
      841 tgccggtggc atggtagcgt tccaggttcc cccgcgcgat ggtagcgtcg gggttgatca
      901 agttgagcgt agccaggaag ccgagagccg ccacgaaagc ccctatcgcg aaacgcttgg
      961 gcttccacca gagggccagc agcagccaca tgaaggtcgc cgccagccac agctcgaaca
     1021 cttccacgta gaggcgcagg tgagtatagc cgtaggcctg ctcgtagagc gacaaacgcc
     1081 agtaggccga ggccagcagc accagcacca gggccaccat gaggctgccc agggcgttga
     1141 agtaaagctt ctgtcgggcc gtgtcgcgcc gggtgagatg gtgcaggccc aggatcaggc
     1201 ccagcgtgag caccgacacg gccacaagct cgaagaaccc ccgccgggcg tactcggcgt
     1261 aggtgaggcc cgccgcgctg atgttgcttt cgccgccgaa caggtacgcg aactggacca
     1321 gcccgaacac caggaatagc gcgttcacca gcaccagtac ggtcgtcact tccacgaagc
     1381 ctatcgaaac gcgggtgggc acggcgtcca taccggcatc ccacggttct tcgtcgccgg
     1441 ccgaggcccc gtcgcgcctg ccgacggcga aggccagccc tcccgccagg acccagccca
     1501 cgatgacagc ccaaacgacc tgcgtgaaga ggtcgcccat gtcggggagg tacctgaact
     1561 caagcatttg gccgagcagg ctggcgaata ccatatccgc tgaagagagc agggccgcga
     1621 acaccaggag caccggggcg gccagcatga cgccgcgcag cacgggcatc cacttgtcgc
     1681 ggctcttgcc gcctagctgc ctgccctcca tgctctttgg caccagcgtg aagggcaggg
     1741 taacagagtg cagccccgcc gtcatcagcg agaggtagta gccgaggatg cccaggcggt
     1801 cgagccgccc cgccgcgtag aaataggcca gcagcaccag gaggccgagc acggcgcaca
     1861 cgttcaggaa cgtcaggaag ccatcgactc ggaagtagac catcgaggcg aagaagcaga
     1921 ttgggagcac gagccacatg ttgcgcatgc gcgggcgcac gtcctcctgg cggcccagcc
     1981 agaacagcac tcccagcagg gaggccacga acagcgggaa cgatatgccg agcatcttcc
     2041 ggtagaagag cacgtcgaag gccatgccca gccccaggcc cgcccatagc acgcgggaag
     2101 gggaggcgag attgggcaca atgcgaatgc caggtgaacg gggcgcactc gcagtagccg
     2161 tgcctgtctg tgcggcgctc ttggcagggg aatcgatggt gagttggctt gccat
//
LOCUS       DASRWZ010000678         3739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_54861,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000678 DASRWZ010000000
VERSION     DASRWZ010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54861"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..994)
                     /locus_tag="VFH60_10745"
     CDS             complement(89..994)
                     /locus_tag="VFH60_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314307.1"
                     /translation="MRGSPTATPAPRNERAPNPGGPRSSWLNLAWKLLLAALILLLMS
                     FASPLSWLLSWTLLNPVMATLLVWGALVVGVASSNLFEGPTPLRLAWGALTVGAYVAL
                     TLPTLVTNAPSRLVDALGPVAALTLVAHLGRRDLQRALRDWALVMSLALYASLGVHAM
                     WLLLGVFGKFGPLVFVLSVLLPPLLFEAILLALRRIGRPTSILPPTVALLLATVLSTV
                     VVSLNLLNRSTELAWLLLFNLIIATLIGTGLVVSYLTRPMMEAASGAHRLHPRAIDLG
                     RALVELSHGPILISLALYLPLRLLR"
     gene            complement(1004..1852)
                     /locus_tag="VFH60_10750"
     CDS             complement(1004..1852)
                     /locus_tag="VFH60_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET6314308.1"
                     /translation="MSEANSDPKPQHVAIIMDGNGRWARQRGLPRLMGHRAGTENIRR
                     IVHACVENDVKYLTVYAFSTENWNRPDSEVSGLMRILAEVIERETPELDREGVQIRHL
                     GHMENVPQALQDAIAYSLETTRHNSRLILSVCFNYGGRDDVVRAVREIVAEGVAPEDV
                     TEELISAHLDTAGTPDPDLIIRTAGEMRLSNFLLWQAAYSEYYSTPTTWPDFDKEAFA
                     EAIREYGRRRRKFGGILPEEYREYAEREGLPFTADAQQADNGTAAPAAADEQAPISNS
                     PDPAAV"
     gene            complement(1912..2469)
                     /gene="frr"
                     /locus_tag="VFH60_10755"
     CDS             complement(1912..2469)
                     /gene="frr"
                     /locus_tag="VFH60_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET6314309.1"
                     /translation="MIEDVQKDAEDRMKKAIEALRRDLQTIRTGRATPALLDRVTVEY
                     YGTPTPLNQLATVAAPEPRLLTVKPWDKGALPLIEKALQKSEMGFNPSNDGQLIRIPI
                     PALTEDRRREMVKVVKHKVEESRVAIRNVRRDVMHDLKEMENEKMISEDEHKRANDKI
                     DALAHKYIAEADHVGEGKEHEVLEI"
     gene            complement(2466..3287)
                     /gene="pyrH"
                     /locus_tag="VFH60_10760"
     CDS             complement(2466..3287)
                     /gene="pyrH"
                     /locus_tag="VFH60_10760"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET6314310.1"
                     /translation="MRRVNTTLPEPRYRRVLLKLSGEALAGEAKSGIDPAVVSSISNQ
                     ISEVVERGVQVAIVVGGGNIWRGIAASAKGMDRATADYMGMLATVLNALALQDALENR
                     GVSTRVQSAIAMHEVAEPYIRRRGIRHMEKGRVVILAAGTGNPYFSTDSAASLRALEL
                     GCEVLLMAKNRVDGVYTDDPFANPDATKFDVVTYRQAIEMDLRVMDTTAMALCMENSL
                     PIIVFNFQEPGNILRIVMGEKVGTTVTTESDDTHPEEVMPVDKTHELQTLRGLQQ"
     gene            complement(3484..>3739)
                     /locus_tag="VFH60_10765"
     CDS             complement(3484..>3739)
                     /locus_tag="VFH60_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314311.1"
                     /translation="MQVASMDPAEVGTLDGSQGGGNNALLDQEYIRDPRKTIRDLVKE
                     AIGKLGENIQVSRFIRFEVGTSGGTEQPGGSANGTIDSGE"
BASE COUNT          632 a         1091 c         1272 g          744 t
ORIGIN      
        1 aaccgtctat aacaatgact ctctttgcgc gttctttgcg tcctttgcgt ctttgcgttt
       61 tcgtctcttt actcggtact cggtactctc atctcaggag gcgtaagggt aggtaaaggg
      121 ccagggaaat caggatgggg ccgtggctga gctccaccag ggcgcgaccg aggtctatgg
      181 cgcgggggtg gaggcggtga gcgcctgagg cggcctccat catgggccgg gttagatagc
      241 tgaccaccag gcccgtgccg atgagggttg ctatgataag gttgaatagc agtagccagg
      301 ccaactcggt cgagcggttc aacagattga gcgagacgac cacggtggac agcacggttg
      361 ccaggagtaa tgccacagtg ggaggcaaaa tgctggtcgg cctgccgatg cgtcgcaggg
      421 cgagcagtat cgcctcgaac aagaggggcg gcaagagcac cgagagcacg aagacgagcg
      481 gcccgaactt gccgaacacg cccaggagca gccacatggc atgcaccccc agcgaagcgt
      541 aaagggccag cgacatgacg agcgcccagt cgcgtagcgc cctctgaagg tcgcggcggc
      601 ccaggtgggc gacgagggtg agggcggcca ccggcccaag cgcatccacc aggcgggagg
      661 gcgcgttggt gacaagggta gggagggtga gggcgacgta ggcacctacc gtaagcgcac
      721 cccacgccag gcgcaggggg gtagggcctt cgaacaggtt gctcgaggct acaccgacca
      781 cgagcgcgcc ccagaccagc agggtagcca tgacggggtt gagcagcgtc cacgacagca
      841 gccaggatag gggactggcg aacgacatca gcaggaggat aagggcggcg agaagaagtt
      901 tccaggcgag gttaagccag cttgagcggg ggcctccagg gttgggggcg cgctcattgc
      961 gaggggcggg tgtggcagtg ggcgaacccc gcatggaccc ccgctagacc gccgccgggt
     1021 cggggctgtt ggagatgggg gcctgctcgt ccgcggcggc aggggcagcg gtgccgttat
     1081 ctgcctgttg agcgtccgcg gtgaagggta agccctcgcg ctcggcgtat tcgcggtact
     1141 cttcggggag gatgccgccg aacttccgtc gcctgcgccc gtactcgcga atggcctcgg
     1201 cgaaggcttc cttgtcgaag tcgggccagg tggtgggggt ggaatagtac tcggagtagg
     1261 cggcctgcca gagcaggaaa ttgctcaggc gcatctcccc ggccgtgcgg ataatcaagt
     1321 cggggtcggg ggtgcccgcc gtgtccaggt gcgccgaaat caactcttcg gtgacgtctt
     1381 cgggcgcgac cccctcggcc actatctcgc gcacggcccg caccacgtcg tcgcggcccc
     1441 cgtagttgaa gcacacgctc aggatgaggc ggctgttgtg cctggtggtc tcaagggagt
     1501 aagcgatggc atcctgtagc gcctggggga cgttctccat gtggcccagg tgccgtatct
     1561 gcacgccctc gcggtcaagc tccggcgtct cgcgctcgat tacttccgcg agtatgcgca
     1621 tcaggccgct cacctcgctg tcgggccggt tccaattctc cgtcgagaaa gcgtacacgg
     1681 tgaggtactt cacgtcgttc tcgacgcaag catgcacgat gcgccggata ttctcggtgc
     1741 ccgcgcggtg ccccatgagc ctgggcagcc cgcgctgtct ggcccaacgc ccgttcccat
     1801 ccatgatgat ggcaacgtgt tggggcttgg gatcggagtt cgcttcgctc ataattttag
     1861 attttagatt ttggattagg acgtaaatct aaaatccaaa atctaaaatg gctagatttc
     1921 cagcacctcg tgttctttgc cttcgcctac gtgatcggct tccgcgatgt acttgtgggc
     1981 gagggcgtcg attttatcgt tggcgcgctt atgctcgtcc tcggagatca tcttctcgtt
     2041 ctccatctcc ttgaggtcgt gcatcacgtc gcggcgcacg ttgcggattg caacgcggga
     2101 ctcttccacc ttgtgcttga ccaccttcac catctcgcga cgacggtcct cggtgagggc
     2161 ggggatgggg atgcggatga gctggccgtc gttggagggg ttgaagccca tctcggactt
     2221 ttggagggcc ttctctatca ggggtagcgc gcccttgtcc cagggcttca ccgtgagcaa
     2281 gcgcggctca ggggccgcta ccgtggctag ctggttcagg ggggtggggg tgccgtagta
     2341 ctcgaccgtg acgcggtcga ggagggcggg ggtggcacgg ccggtgcgga tcgtctgaag
     2401 gtcgcggcgc agggcctcta tcgccttctt catacggtct tcggcatctt tttgtacgtc
     2461 ttcgatcatt gctgcaatcc tcgcagggtc tgtaactcgt gagtcttgtc tacgggcatc
     2521 acttcctccg ggtgcgtatc gtcagattcg gtggtcaccg tggtgccgac cttctcgccc
     2581 atgacgatgc ggaggatgtt gcccggctcc tggaagttga acacgatgat ggggaggctg
     2641 ttctccatgc acagggccat agcggtggta tccatcacgc gcaggtccat ctcaatcgcc
     2701 tgccggtagg tgactacgtc gaacttggtg gcgtcggggt tggcgaaggg gtcatcggtg
     2761 tacacgccgt ccacgcggtt cttcgccatg agcagcactt cgcagccaag ctccagcgcg
     2821 cgcagggagg ccgccgagtc ggtgctgaaa taaggattgc ccgtgcccgc cgccaggata
     2881 acgacgcggc ccttctccat gtggcggata ccgcgcctgc ggatgtaagg ctcggctacc
     2941 tcgtgcatgg cgatggccga ctgcacccgc gtggacacgc cccggttttc gagggcgtct
     3001 tgcagggcga gggcgttgag cacggtagcc agcatgccca tgtagtcggc ggtggcgcgg
     3061 tccatgccct tggcggaggc ggcgatgccg cgccagatgt taccgccacc caccacgatg
     3121 gcgacctgta cgccgcgctc gaccacctcg ctaatctggt tggagatgga gctgaccacg
     3181 gcggggtcga tgcccgactt ggcctcgccc gccagcgcct cgccgctcag cttgagcagc
     3241 acccgcctgt aacgcggctc cgggagcgtg gtgttcacgc gcctcatggc gggtagttct
     3301 tctacatcat cgtcatcagg caacagtgga tcgatcaagg ggacctcctg gtgcaggctg
     3361 ccatgctgcc taatagtcta tcaggtttgg cagtcgatga caacaacctg agtgcttgac
     3421 gggaaggcta atgcgtaaca cgtagctccc gagtagggtg gcttacgtgt tacgcgttac
     3481 gtgttactct cccgagtcta tggtaccgtt agcagacccg cccggctgct cggtgccgcc
     3541 ggaggtgcct acctcgaagc ggatgaatcg gctgacctgg atgttctcgc ccagcttgcc
     3601 aatggcttcc ttgacgaggt cgcggatggt cttgcggggg tcgcggatgt actcctggtc
     3661 gaggagggcg ttgttgccgc cgccctggct gccgtcgagg gtgcccactt cggcggggtc
     3721 catcgaggct acctgcatg
//
LOCUS       DASRWZ010000679         1665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_672220, whole genome shotgun sequence.
ACCESSION   DASRWZ010000679 DASRWZ010000000
VERSION     DASRWZ010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672220"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VFH60_10770"
     CDS             complement(<1..796)
                     /locus_tag="VFH60_10770"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET6314312.1"
                     /translation="MANEPVQGDGRRPHSSDGEAANLVIEQGSGLPLAGVGTDVLDHT
                     PGRTNRPIKKVGFIVNDTIEAAKVEAPRIAGLLHERGVEVTESHSASPDTTVNWTKDD
                     LLDMIFTFGGDGTILRAARIAAPLGVPLMGVNLGRVGFLTEMNPWQVQERIDLLREGK
                     YWLERRAMLRAVLLRDSVEVGSFVALNDVVASRAALSRVVHCTLSANGHKVTTYVADG
                     VIVATPTGSTAYSMAAGGPIMHPELRSIIVTPIAPYLTIVKSLVLPD"
     gene            complement(786..1577)
                     /locus_tag="VFH60_10775"
     CDS             complement(786..1577)
                     /locus_tag="VFH60_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET6314313.1"
                     /translation="MKQRLDVALVERGLAETRARAQAIIMAGDVQVDGQRADKPGMPV
                     AEGARIEVKATLPYVSRGGLKLAHAIEAFGLAGRIAGKVALDIGASTGGFTDVLLQAG
                     AAHVYAVDVGTNQLAWKLRQDPRVTPMEQTNIRYLDALPQPCDLATCDVSYIGLELVT
                     PPAVRLTSPDAFFVFLIKPQFEAGREQVGKGGVVREASVHRDVIIRLAHFWRALGLHL
                     AGLARSPITGPAGNVEYLALIEKQPRPEAFDLQAAIEREVKLDGK"
BASE COUNT          285 a          553 c          546 g          281 t
ORIGIN      
        1 cgtcggggag caccaggctc ttgacgatgg tgaggtaggg cgcgataggc gtgactatga
       61 tgctgcgcag ctccgggtgc atgatggggc caccggcggc catggaatag gcagtggagc
      121 cggtaggggt agccacgatg acgccgtcgg ccacgtaagt agtgacctta tgcccgttcg
      181 cgctgagcgt gcagtgcacc acgcgggaga gggccgcgcg gctggctacc acgtcgttca
      241 gcgccacgaa gctgcccacc tcaacgctgt ccctcagcag cacggcccgc agcatggccc
      301 gccgttcgag ccagtacttg ccttcgcgca agaggtctat ccgctcctgc acctgccagg
      361 ggttcatctc ggtgaggaag cccacccgcc ccaggttcac gcccatcagg ggcacgccca
      421 gcggggccgc gatgcgggcc gccctcagga tggtgccgtc gcccccaaac gtgaagatca
      481 tatcgagaag gtcgtctttg gtccagttga cggtggtatc gggcgaggcg gagtgcgatt
      541 ccgtcacctc cacgccgcgc tcgtggagca gcccggctat ccggggggcc tcgaccttgg
      601 ccgcctcgat ggtgtcgttg acgatgaagc cgaccttctt gatagggcga ttagtgcggc
      661 cgggggtatg gtccaggacg tcggtgccca cgcccgcgag gggcaggccg ctaccctgct
      721 cgatcaccag gttggcagct tcgccatctg agctatgggg tctgcggcca tcaccctgta
      781 ccggctcatt tgccatccag tttgacctct ctttcaatcg cggcttgcag gtcgaacgcc
      841 tcagggcgtg gctgcttctc gataagggcc aggtactcca cgttgcccgc cgggccggta
      901 ataggcgagc gggccaggcc ggccaggtgc agccccagcg cccgccaaaa gtgcgctagc
      961 cgtattataa catcgcggtg tacgctcgcc tcgcgcacca cgccgccttt gcccacctgc
     1021 tcgcggcccg cctcgaactg cggcttgatg aggaacacga agaaggcgtc aggggaggtc
     1081 aggcgcacgg cggggggcgt cacaagctca agccctatat atgatacgtc gcaggtggcg
     1141 aggtcgcacg gttggggcag cgcatctaag tagcggatgt tggtctgctc cataggggtg
     1201 acgcgggggt cctgccgcag cttccaggca agctggttgg tgcccacgtc cacggcgtac
     1261 acgtgggccg cgcccgcttg cagcagcacg tcggtaaaac cccctgtgga ggcccctatg
     1321 tcgagggcca ccttgcccgc gatgcgcccc gccagcccga acgcctcaat cgcgtgcgcc
     1381 agcttcagcc cgcccctgct cacgtagggc agggtcgcct tcacctcgat gcgtgccccc
     1441 tcggccacag gcatgcccgg cttgtcggcc cgctgcccgt ccacctgcac atcgcccgcc
     1501 ataatgatgg cctgcgccct ggcacgggtc tccgccagcc cgcgttccac gagcgctacg
     1561 tcaagtcgct gcttcataag agaacaatag cacgaaaacg caaagacgca aagagcgcaa
     1621 aggacacgca aagggggtga tggtttgcgg acggcaagcg ccagg
//
LOCUS       DASRWZ010000680         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1152020, whole genome shotgun sequence.
ACCESSION   DASRWZ010000680 DASRWZ010000000
VERSION     DASRWZ010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152020"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFH60_10780"
     CDS             complement(<1..207)
                     /locus_tag="VFH60_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314314.1"
                     /translation="MTITLEWWQYILPAFIACGVVGLYMGWRIMGLWTVGVFFSGLVA
                     ARLGPKLELFINKFFSVGGEFFAIA"
     gene            complement(241..1416)
                     /gene="ftsW"
                     /locus_tag="VFH60_10785"
     CDS             complement(241..1416)
                     /gene="ftsW"
                     /locus_tag="VFH60_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET6314315.1"
                     /translation="MSRARRFERPDYWILAAIGILCMIGVLMVFSSSGVEEGDPTRLV
                     TRHAMMLSIGTFALLVTMSVPYTRWRRYSVLGIAVALILLVAVLVLPPFFSETINGAK
                     RWLSIGNLPFGLQPSEFAKLALVIYLADWCSTKGEKVRDLTNGLVPFGIVLGAMAGLV
                     FIEPDMGTMLVIFSIGVTIYFVSGAAMSHMIVGFLGALIAFAIAATAAPYRMQRVLSF
                     LDPWHDPQGVSYHSVQSLLALGSGGWTGMGLGSGRQKYGWLPEQSTDAIFSVWGQEVG
                     FVGAVLMIALFLVVAWRGYRVARLAPDGFSSLLATGITTWIIFQGMLNIGAVSGAVPF
                     TGVPLPFISYGGSSLVILLTGVGILLNISKHANLPVTETRNAGNAASRVLRLEPKAR"
     gene            complement(1869..3263)
                     /gene="murD"
                     /locus_tag="VFH60_10790"
     CDS             complement(1869..3263)
                     /gene="murD"
                     /locus_tag="VFH60_10790"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET6314316.1"
                     /translation="MNFPYADKNVLVLGLGVHGGGLGVARFRGRNGANVRVTDLRSAE
                     QMQESVDALARESFAVEYTLGEHREVDFQWADVVVRNQAVPLTSPWVALARQLGKPVE
                     TEINIFMRLCPGPILGVTGTKGKSSTATWTWEMVRHWREDAVLAGNLRVSALEALDRI
                     TPETPVVLELSSFQTEGFEEDRLAPHLAAVTNLSPDHLDRYRSMEEYGAAKKHIFLYQ
                     QPERGDYVVLNAADPVVAGWAEDALAGVAWFGIGAATRTPGVYVQDGVFYWYEQGREH
                     VFVGEAADLQVPGAHNLANAACAATLAILAGAPLEAVRDGLRSFKGVADRQELLRTLR
                     GVRFYNDTTSTTPTSTIAALNAIEGPIVLISGGADKGLDFAQLAPVVTAETVGVVLLE
                     GTVTDLMESQFREVGVNLLGRFSDFGEAVRVAQEAAPEGGAVLLSPGTASFGMFRNEF
                     HRGEQFRAIVAGLT"
BASE COUNT          655 a         1084 c         1044 g          564 t
ORIGIN      
        1 ggctatcgcg aagaactcgc ccccgactga gaagaacttg ttgatgaaca attccagctt
       61 ggggccgagc cgggcggcca ccagaccgct gaaaaagact cccaccgtcc acagccccat
      121 gatgcgccag cccatgtaga ggcccaccac gccgcacgcg atgaacgcgg ggaggatgta
      181 ctgccaccat tcgagcgtga ttgtcatggg gcgtctctcc tgggcatggc gctcatccct
      241 ttacctcgcc tttggctcaa gcctgagcac cctcgaggct gcgttgccgg cgttacgcgt
      301 ctcggtcacg ggcaggttgg cgtgcttgga gatgttgagc aggatgccca cgccggtaag
      361 caggatcacg agggacgagc cgccgtagct gataaagggc aggggcaccc cggtgaaggg
      421 caccgccccg ctcaccgcgc cgatgttgag catgccctgg aagatgatcc aggtcgtgat
      481 gccggtcgcc agcagtgacg agaagccgtc gggggcgagc cgggccacgc gatagccacg
      541 ccaggccacc accaggaaga gcgctatcat cagcaccgcc ccgacgaagc cgacctcctg
      601 cccccacacc gagaagatgg cgtcggtcga ttgctcgggc agccagccgt acttctgccg
      661 ccccgacccc aggcccatgc ccgtccagcc accggaaccg agggccagca gcgactgcac
      721 cgagtggtag ctcaccccct gggggtcgtg ccacgggtcg aggaaggaca gcacgcgctg
      781 catgcggtag ggggcggccg tggccgcgat agcgaacgcg atcagggccc ccaggaaacc
      841 cactatcatg tgcgacatgg ccgcgcccga cacgaagtat atagtcactc ctatcgagaa
      901 gatgacgagc atggtgccca tgtccggctc gatgaacacc aggcccgcca tcgcgcccaa
      961 cacgatgccg aagggcacca ggccgtttgt gaggtcgcgc actttctcgc ccttggtgga
     1021 gcaccagtcg gccaggtaga tcaccagggc cagcttggcg aactcggagg gctgtagccc
     1081 aaacggcaga ttgcctatgg agagccagcg cttggcccca ttgatggttt cggagaagaa
     1141 tggcggcagc accagcaccg ccaccaacaa tattaaggca accgcgatgc ccagcacgga
     1201 gtacctgcgc cagcgagtgt agggcacgct catcgtcacc agcagggcga acgtgccaat
     1261 gctaagcatc atggcgtggc gggtgaccag gcgcgtgggg tcgccctcct cgactccgct
     1321 ggaggagaag accatgagca cgccaatcat gcacaggatg ccgatagcgg ccagtatcca
     1381 gtagtcgggt cgttcgaagc gtcgggcacg agacatagtg gtggatttcg gatttggaaa
     1441 ttcggatttc ggatttgtgg gagggccagg atatgttctt gccttccagg gagtccatct
     1501 tagcgcaccg ggggcgggcg agggccgtta tccacaggtg gggaatacgt ccgaaggaga
     1561 cgggaccgcc aggacgccaa ggatgccaag agtgcgccaa gaagaagagg atcattcacc
     1621 gcagagacgc agagagcctg ccctgagcgc agccgaaggg gcgcagagga cacgctgaga
     1681 ttaggattgt ttaaggatag acaaaggcag catcgtacag tacgatgctg cccttgctag
     1741 aacaccctca attcttctct gtgtaccttt gcgtcccttc ggctgcgctc agggcaggct
     1801 ctctgcgtct ctgcggtgaa ccgttcttat tcctcttggc gtcctcggcg tcttggcggt
     1861 ttcgttcttt acgtaagccc tgcgacaatc gcgcggaact gttcgccccg gtggaactcg
     1921 ttgcggaaca tgccgaagga ggcggtgccc ggcgacagca acacggcccc accctccggc
     1981 gcggcctctt gagcgacccg caccgcctcg ccgaaatcgg agaaacggcc caggaggttt
     2041 acccccacct cgcggaactg gctctccatg aggtcggtga ccgtgccttc cagcagcacc
     2101 acgcccaccg tctcggccgt gaccaccggc gcaagctgcg cgaagtcgag gcccttgtcc
     2161 gccccgcccg atatgagcac gataggccct tcgatggcgt tcagcgcggc gatggtggag
     2221 gtcggtgtag tagaggtggt atcgttgtag aaacgcacgc cgcgcaacgt ccgcaacagt
     2281 tcctgccggt cggctacccc cttgaaggag cgcaggccgt cccgcactgc ttccaggggc
     2341 gcccccgcca ggatagccag ggtagcggcg caggctgcgt tcgccagatt gtgggcgccg
     2401 ggcacctgca agtccgcggc ttcccctacg aagacgtgct cccggccttg ctcgtaccag
     2461 tagaacacgc cgtcctgcac gtagacaccg ggcgtcctgg tggcggcccc tatgccgaac
     2521 caggcgaccc cggcgagggc atcctcagcc cagcccgcca ctaccgggtc ggctgcgttg
     2581 agcaccacgt aatcgccgcg ctccggctgc tggtagagaa agatgtgctt cttggccgcg
     2641 ccgtactcct ccatgctcct gtagcggtcg aggtggtcgg gcgagaggtt ggtgaccgcg
     2701 gccaggtggg gcgcgagccg gtcttcctcg aatccctcgg tctggaagct cgatagctca
     2761 agcaccacgg gggtttcggg tgtaatgcgg tcgagggcct ccaacgcaga tacccggagg
     2821 ttgcccgcca gcaccgcgtc ctcccgccag tgccgcacca tctcccacgt ccaggtggcg
     2881 gtggacgact tgcccttggt gcccgttacg ccaaggatag ggccggggca cagccgcatg
     2941 aagatgttga tctcggtctc cacgggcttg ccaagctgcc gcgccagggc cacccagggt
     3001 gaagtgaggg gcaccgcctg gttgcgcacg accacgtcgg cccactgaaa atcgacctcg
     3061 cggtgctcgc ccagcgtgta ctccaccgcg aagctctccc gcgccagggc atcaaccgac
     3121 tcctgcatct gctcggcgct acgcaagtcg gtcacgcgca cgttggcccc attgcgcccc
     3181 ctaaaccgcg caacccccaa cccgcccccg tgcaccccga ggcccagaac aagtacgttt
     3241 ttgtcggcgt aaggaaaatt catacgaatt tggaatttgg aatttcggat ttcgaattta
     3301 ctaggatttc gaacaaatcc gaaattcgaa atccgaaatt ccaaatt
//
LOCUS       DASRWZ010000681         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_124124, whole genome shotgun sequence.
ACCESSION   DASRWZ010000681 DASRWZ010000000
VERSION     DASRWZ010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124124"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1181
                     /locus_tag="VFH60_10795"
     CDS             168..1181
                     /locus_tag="VFH60_10795"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6314317.1"
                     /translation="MEHFTNGTGREVRHGEQRRANMTGAVVLGLVLCFTVLAAALVVF
                     AGRASAIDSREAARVGSVQGIPAAQETVPAAVDTGVAQPVQTGGTGPVGGAVASVGPT
                     AANQGVAAATQGGDFPWWILIPILLALAVVAFLVARRRPAVAVTTAPGARGAGPYTGT
                     TTTTTTYTTTSTAGLGAATTAVGAAGAATPTEIVCPNCGATNDLNENFCHDCGQDLRQ
                     ARAQLLAPALDVVDEYTPYLETQSRVDEQLEYVLSRGRVTVGSAPGNDIVIDAAFNGS
                     GTVGGTHAELRREGDGFVIADLGSGTGVYVNGDKVAEAALKDNDQVRVGDVQFVYHAP
                     PRP"
     gene            1215..3485
                     /locus_tag="VFH60_10800"
     CDS             1215..3485
                     /locus_tag="VFH60_10800"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET6314318.1"
                     /translation="MNRLRRAGQTNNNAEVTGDESRAERAPQQQEAAGTNGTPDELVM
                     DAGDASFFRESSTESAEPAFGAAAVLDTESAGLHSHLDIAETTPPEPTYPNASPDDAG
                     SEAGQGAEGEIVAQDATTTTRPDEAPTQPNVLISDGTAPLSSMVEPETLEPVPVNGLI
                     ASRYMVQNILHHGIDRNLYRVGAHKQQRCLTCGRLSPMDQEVCDTCKSPLANQAPADF
                     YLMAESYRPESLLQDPDLMQLSLYHPNLVPVIDFFSDTPHGRERFYAVAEPRQGVRLS
                     QLSLPRSGLQVLNWAMQLADALDYLHSRGVVGAGAEADDILVQGDRASLASLQNARAS
                     DPNTDPADMQAMDLARLASTIYEAYTGNQVSMGPDGTLAMPSGTPEPVGAAFRAAIEP
                     VQGGGRIISAAQWRNLLASAMETIDELERPGRPVDFVSAGLTDVGRLREQNQDSFGMA
                     EFVQASAERPMRVGFYMVADGLGGHKGGEIASALAVQAFSGEVVSRIIAPIALSNGDR
                     STPTNEAILQAMTRAVQLANERIYKARDSRRNDMGTTLVAAVIAGGKAYVVNVGDSRI
                     YMYARNRGQMDAQMKDATRPLIKGTSPLSGTAPLRSKEAAMDALEEALESRGVSASET
                     GSFALNQVSIDHSLVHRLVELGQLEPEEAKVHPHRNFIYRSLGGPPPIEVDTFVRTLH
                     PGDRLMLCSDGLNSMVEDEDIEHILAVEPDPHNACRRLIDLANQNGGHDNITVIIVDV
                     TEYLPAPEHPHALLGQ"
BASE COUNT          689 a         1114 c         1154 g          580 t
ORIGIN      
        1 tgaactttat acttggaaat taggaattag gatttaggat ttaggatttt agaaggtatc
       61 cgaacaaatc cagaatccaa aatccaaaat cctaaatctc aaagtggtat aatatcctcc
      121 gggcttgcga ggcttgccta atatagcaac tgggggagtt acctcttttg gagcatttta
      181 ctaacggcac gggacgcgag gtccggcacg gtgagcagcg ccgcgccaac atgacgggtg
      241 cagtggtcct gggtctcgtg ctttgtttta ctgttcttgc tgcggcgttg gtagttttcg
      301 cgggtagggc atcggccatc gatagtagag aggccgcgcg ggtgggaagc gtacaaggca
      361 tcccggcggc ccaggagacg gtccccgcgg cggtggatac gggcgtggcc cagccggtac
      421 agaccggtgg aacgggaccc gtcggcgggg ctgtggcctc ggtgggtccg accgccgcca
      481 accagggcgt ggccgcggcc acgcagggcg gggacttccc ctggtggata ttgataccga
      541 tattgctcgc tttagcggta gtggcgttcc tggtggcgcg gaggcgtccg gcggtggcgg
      601 ttacgacagc acccggagca cggggcgcgg ggccttacac tggcaccacg accactacta
      661 caacgtacac aacgacatcg acagccgggc tgggggccgc cacaaccgcg gtgggcgccg
      721 ccggggcagc taccccgact gagattgttt gccccaactg cggtgccact aacgatctga
      781 acgagaattt ctgccatgat tgcgggcagg acctcaggca ggcgcgggcg cagttgctcg
      841 ccccggcatt ggacgtagta gacgagtaca cgccgtacct ggagacccag agccgcgtgg
      901 acgagcaatt ggagtatgta ctatcgcggg ggcgggtcac tgtgggcagc gcgcccggca
      961 acgacattgt gattgacgcg gccttcaacg gctcagggac cgttggtggc actcacgctg
     1021 aattgcggcg tgaaggtgac gggttcgtga tcgcggacct cggttcaggg accggagtgt
     1081 acgtgaacgg ggataaagtc gctgaagctg ctttgaagga caacgaccag gtacgcgtag
     1141 gcgatgtgca gtttgtgtat cacgccccgc cgcggccata aggttatgtg caaataggct
     1201 cactgggaga gtatatgaat cgacttcgcc gcgccgggca gaccaacaac aacgccgaag
     1261 ttactggaga cgagtcgcgg gctgaacgag caccccagca gcaggaagcc gcagggacta
     1321 atggtacccc tgacgagctt gtcatggatg ccggggacgc cagcttcttc cgtgaatcgt
     1381 ctaccgagtc ggcggagcct gctttcgggg cagcagccgt gcttgacacg gagtcggcgg
     1441 gtttgcactc gcacctggac atcgccgaga cgaccccgcc cgaaccgacc tatcccaacg
     1501 catctcctga tgacgccggg tccgaggccg gtcaaggggc ggagggcgag attgtagcac
     1561 aggacgctac gaccactacg cgccccgacg aggcccccac tcagcctaac gtgctcataa
     1621 gcgacgggac ggcacccctg agcagcatgg tcgagcctga gacgctcgag ccggtgcccg
     1681 tgaacggcct catcgcgagc aggtacatgg tgcagaacat cctgcatcat ggcatcgacc
     1741 gtaacctcta ccgcgtgggc gcgcacaagc agcagcgctg cctcacctgc ggccgcctct
     1801 cccccatgga ccaggaggtt tgcgacactt gtaagtcccc tctggccaac caggcaccgg
     1861 ccgacttcta cttgatggcc gagagctacc ggcccgagtc cctgttgcaa gaccccgacc
     1921 tgatgcagct cagcctgtac caccccaacc tggtgcccgt catcgacttc ttcagcgaca
     1981 cgcctcacgg ccgcgagcgg ttctacgcgg tggccgagcc tcgccagggc gtgcgactga
     2041 gccagcttag cctgccccgg tcgggcttgc aggtgctcaa ctgggccatg cagcttgccg
     2101 acgcgctcga ctacctgcac agccgcgggg tcgtgggcgc gggggccgag gcggacgaca
     2161 tcctggtgca gggcgaccgg gcctcgctcg cgagcttgca aaacgcccgc gcatccgatc
     2221 ccaacaccga ccccgccgac atgcaggcta tggacctcgc acgcctcgcc agcaccatct
     2281 acgaggccta cacgggcaac caggtgtcga tggggccgga cggtacgctc gccatgccct
     2341 ccggcacgcc ggagcctgtg ggcgcggcct tccgcgcagc gatagagcct gtgcagggtg
     2401 gcggccgcat catcagcgcg gcccagtggc gcaacctgct ggcctcggct atggaaacga
     2461 tagacgagct agagcgcccc ggcaggccgg tggacttcgt atcggccggg ctgacggacg
     2521 tggggcggtt gcgcgagcag aaccaggact cgttcggcat ggccgagttc gtgcaggcca
     2581 gcgcggagag gcccatgcgc gtcgggttct acatggtggc cgacgggctg ggtgggcaca
     2641 aggggggcga gatcgcatcc gcccttgcgg tgcaggcgtt ctcaggcgag gtggtctcgc
     2701 gcataatcgc ccctatcgcc ctgtctaacg gcgaccgctc gacccccacc aacgaggcga
     2761 tattgcaggc catgaccagg gcggtacagc ttgccaacga gcgcatttac aaggcgcgtg
     2821 acagccgccg caacgacatg ggcaccaccc tggtggcagc ggttattgcc gggggcaagg
     2881 cgtacgtggt gaacgtgggc gacagccgca tctacatgta cgcccgcaac cgtggccaaa
     2941 tggacgccca gatgaaggac gcgacccgcc cgctcatcaa gggcacctct ccgctatccg
     3001 ggacggcgcc ccttcggtcg aaagaggccg caatggatgc gctggaggag gcgctggaga
     3061 gccggggtgt cagcgcaagc gagaccggct cgttcgccct caaccaggta tccatcgacc
     3121 attcgctggt gcacaggctg gtggagctag gacagttgga gcctgaggag gccaaggtcc
     3181 acccgcatcg taatttcatc taccgctcgc tgggcggacc accccccatc gaggtggata
     3241 cgttcgtgcg cacgctgcac cccggcgacc gcctgatgct ctgttcggac ggcctcaaca
     3301 gcatggtgga ggacgaggac atcgagcaca ttctcgcggt cgagcctgac ccccacaacg
     3361 cctgccgccg cctgatagac cttgccaacc agaacggcgg gcacgacaac atcaccgtca
     3421 tcatagtaga cgtcaccgag tacctccccg cccccgaaca cccgcacgct ctgcttgggc
     3481 agtaatttca ggagacggaa ccgccaagac gccaaggacg ccaagagcgc gccaaga
//
LOCUS       DASRWZ010000682         1792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1289492, whole genome shotgun sequence.
ACCESSION   DASRWZ010000682 DASRWZ010000000
VERSION     DASRWZ010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289492"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VFH60_10805"
     CDS             <1..1019
                     /locus_tag="VFH60_10805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6314319.1"
                     /translation="ITFYEPDAYERQQHRDIPDPEWATVVVYPGEGEEGAYRVLEAAR
                     HADVVVKASGVGVFDELLEREVLSLQRPGTLVVYCDVDAPATLERVHGDPNDPFLPLI
                     PRYDLVFTYGGGDPVVNAYKALSARDCVPIYNALDPSTHYPVSPDPRFEGDLGFLGNR
                     LPDREARVEEFFLRAASLLPGQSFLLGGSGWDGKGMPPNVNYVGHVYTRDHNAFNCTP
                     RMVLNVSRESMARYGFSPATRVFEAGGAAACLITDYWEGIDHFFEPGTEVLVARDGSE
                     VAEHVRGTGVEQVRRIGEAAYRRVLSEHTYAHRAAQLEALLTGSLLAPAGGPSNAQAT
                     GAAR"
     gene            1019..>1792
                     /locus_tag="VFH60_10810"
     CDS             1019..>1792
                     /locus_tag="VFH60_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6314320.1"
                     /translation="MPESLNIVILGLSITSSWGNGHATTYRGLVRELTRRGHNVLFLE
                     RDKPWYASNRDMPDPPWGRTRLYDSLDDLRACYTAEVRDADLVIVGSYVPEGVAVGEW
                     VTQTARGATAFYDIDTPVTLAKLERGDTEYLAPHLIPRYGMYLSFTGGPTLARIERQY
                     GSPMARALYCSVDPELYYPISNFGFRISDLDEFGANSKSEIRNSKSYDMGYMGTYSDD
                     RQPPLDLLMLEPARRWAEGRFVVAGPQYPEHIVWPRNVRR"
BASE COUNT          304 a          624 c          560 g          304 t
ORIGIN      
        1 gcatcacctt ctacgagccg gacgcctatg agcgacagca gcaccgcgac atccccgatc
       61 ctgagtgggc gaccgtggtg gtctacccag gcgagggcga ggagggtgcg taccgcgttt
      121 tggaggccgc ccgtcacgcc gacgtggtcg tcaaggccag tggtgtgggg gtgtttgacg
      181 aactgctgga gcgcgaggtg ctttcgcttc agcggcccgg cacgctggtg gtctactgcg
      241 acgtggacgc gcccgccacc ctggaacggg tgcatggcga ccccaacgac ccgtttctac
      301 cgctcatccc tcgctacgat cttgtcttta cgtacggcgg gggcgacccg gtggtgaacg
      361 cctacaaagc cctcagcgct cgcgactgcg tgcccatcta caatgccctc gacccctcca
      421 cccactaccc ggtgtcgccg gacccccgct tcgagggcga cctgggcttc ctcggcaacc
      481 gcctccccga ccgcgaggcc cgcgtggagg agtttttcct cagggccgcc tcgctcctgc
      541 ccggccagag ctttctactg ggtggcagcg gctgggacgg caaggggatg ccccccaacg
      601 tcaattacgt gggccacgta tacacccgcg accacaacgc cttcaactgc accccgcgca
      661 tggtgctcaa cgtcagccgg gagagcatgg cccgctacgg cttctccccc gccacccgcg
      721 tgttcgaggc aggcggggcc gccgcctgcc tcatcaccga ctattgggaa ggcatcgacc
      781 acttcttcga gccgggcacc gaggtgctgg tggcgcggga cggctcggaa gtggccgagc
      841 acgtgcgcgg caccggcgtg gagcaggtgc ggcgcatagg cgaggcggcc taccgccgcg
      901 tgctgtccga gcatacatac gcccaccgtg cggcgcagtt ggaggcgttg ctcaccggca
      961 gcctgcttgc cccggcaggc ggcccctcca atgctcaagc tacaggagca gcccgctgat
     1021 gccggagagc ctgaacatag tcatactggg cctgtccatc acctcctcct ggggcaacgg
     1081 ccacgccacc acctacaggg gcctggtgcg cgagcttacc cggcgcggcc acaacgtgct
     1141 cttcctggag cgggacaagc cctggtacgc ctccaaccgc gatatgcccg accctccctg
     1201 gggccgtacc aggctctacg acagcctgga cgacctcaga gcctgctaca ccgccgaagt
     1261 gcgcgacgcc gacctggtga tagtcggctc ctacgtgccg gagggcgtgg ccgtgggcga
     1321 atgggtcacg cagaccgcca ggggggccac cgccttctac gacatcgaca cccccgtgac
     1381 gctggcgaag ctggagcgcg gcgacaccga gtacctagcc ccccatctca tcccccgcta
     1441 cggcatgtac ctatccttca ccggcggccc cacactcgcc cgcatagagc gccagtacgg
     1501 atcccccatg gcccgcgccc tctactgctc cgtagacccg gaactgtatt atccgatttc
     1561 gaatttcgga tttcgaattt cggatttaga tgaattcgga gcaaattcga aatccgaaat
     1621 tcgaaattcc aaatcctacg acatgggcta catgggtact tatagcgacg accggcagcc
     1681 tccactcgat ttgctgatgt tggagcctgc gcggcggtgg gcggagggca ggtttgtggt
     1741 ggcggggccg cagtaccctg agcatatcgt gtggccgcgg aacgtgcggc gg
//
LOCUS       DASRWZ010000683         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_946543, whole genome shotgun sequence.
ACCESSION   DASRWZ010000683 DASRWZ010000000
VERSION     DASRWZ010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946543"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFH60_10815"
     CDS             <1..522
                     /locus_tag="VFH60_10815"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET6314321.1"
                     /translation="SGSLSATGHFNLVAAIAAATIGSLMGASVLYGIGRWGGETRIGD
                     WLDRYGKWVLLSRDDLHNSRTWFAKHGNYAVLVARVIPGMRSFVSVPAGLASMHYGRF
                     LLFTAIGSAIWNAGLVLAGYFLGSNWSQVEGWLAPLGPVIYVIMALVLGLFVGRRLWT
                     KFGPNARRTKPVE"
     gene            683..907
                     /locus_tag="VFH60_10820"
     CDS             683..907
                     /locus_tag="VFH60_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314322.1"
                     /translation="MFSELTRAIYDSSGMDWQTAEKIAYTVLVFMEKKMPLEDYNILK
                     RYLLGDVEYKMPDRSIYPGYGGELPDQART"
     gene            1006..>2132
                     /locus_tag="VFH60_10825"
     CDS             1006..>2132
                     /locus_tag="VFH60_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6314323.1"
                     /translation="MRPGVYKIATVGGIDIKLHWSWLLILLLMTFSLSSSWFPMRLPN
                     ENPLTYWGLGLVASLMLFVSVLVHELAHSFVALARGLKVHDIVLYMFGGASNIESEPE
                     KPSDEFIIAVVGPLTSLGLSGLFWLLTVALTPQEGQLGSWGANLFGYLAVINLLLAVF
                     NLIPGFPLDGGRVLRAGIWGLLKDFDKATRYAAMISQFVAYGFIAGGLFWAFFLGDWD
                     GLWLAFIGWFLLNAAQQSVTGVRVREAVKGVTVAQVMEQTPPVVAPTYTIAHLLSQYI
                     LPRNLRVVPVAYDGRLVGIVTLSDIKDIPQEEWGTGPAADVMPGGERLRVAHPSDRLD
                     KVMDTLMESDFDQLPVVDARGALVGMLTRAHILRWLKLREEL"
BASE COUNT          354 a          634 c          694 g          450 t
ORIGIN      
        1 tcgggcagcc ttagcgccac cgggcacttc aacctggtgg cagctattgc ggcggccact
       61 atcggctcgc tcatgggcgc aagcgtcctt tacgggatcg ggcgatgggg cggcgagacc
      121 cggattggcg actggctcga ccggtacggc aagtgggtgc tcctctcacg cgacgacctg
      181 cataactcgc gcacgtggtt cgccaagcat ggcaactatg ccgtgctggt ggcgcgtgtc
      241 attcccggta tgcgctcgtt cgtgtcggta cccgcggggc ttgcctccat gcactacggc
      301 cggttcctgc tgtttaccgc gattggcagc gccatctgga acgccggcct cgtcctggcc
      361 gggtacttcc tgggcagcaa ctggagccag gtcgaagggt ggctggcccc tctcggcccg
      421 gttatttacg ttattatggc gctcgtgctg gggttgttcg tggggcggcg gctatggacc
      481 aagttcggcc ccaacgccag gcgcaccaag cccgtggaat gagccttgca tgaaaccgat
      541 ttgggatttc gaattgcaga gttcgacttt gttagctttc gggtgctact ttgtgccgac
      601 taagcggggc gggcgttgcg ccgggtgttg gggtgtgtta tgttacgtgt aattttgctc
      661 acgaagccgg gaggagagaa caatgtttag cgagcttacc agggccattt acgactcgtc
      721 gggtatggac tggcagaccg ccgagaagat cgcatacacg gtgttggtgt tcatggagaa
      781 gaagatgccc cttgaggact acaacatcct gaagcggtac ctgctgggcg acgtggagta
      841 caagatgccg gacaggtcta tctaccctgg ctacggggga gagttgccgg accaggcacg
      901 gacgtagacc ctggtgcctg tgccgcccgt tattatcacg gtcctctcgc gagagcggct
      961 tctcgatgcc ttatccttga cggcggcagg aactgagagg tcgcgatgag gccgggcgtc
     1021 tataagattg ccacggtcgg cggcatcgac atcaagctgc actggagctg gctgctcatc
     1081 ctgctcctga tgaccttcag cctctcaagc tcctggttcc ccatgagact gcccaacgaa
     1141 aaccctctca cctactgggg gctgggactg gttgcctcgc tcatgctctt cgtatcggtg
     1201 ctggtgcacg aattggcgca ctcgttcgtg gcgttggcgc gagggctaaa ggtgcacgac
     1261 atcgtgctct acatgttcgg gggcgcgtcc aacatcgaga gcgagcctga gaagccgagc
     1321 gacgagttca tcatcgccgt ggtggggccg ctcaccagcc tgggcctctc aggcctcttc
     1381 tggctgctaa ctgtggccct tactccccag gaaggtcagc tcggaagctg gggcgcaaac
     1441 ctattcggct acctggctgt gatcaacctc cttctggcgg tgttcaacct gatccccggc
     1501 tttcccctgg acggcggacg ggtgctgcgt gcaggcatct ggggccttct gaaggacttc
     1561 gataaggcca cgcgttacgc ggcgatgatc agccagtttg tggcttacgg tttcatcgcg
     1621 ggtggcctgt tctgggcgtt cttcctgggc gactgggacg gcctatggct tgccttcatc
     1681 ggctggttcc tgctgaacgc cgcccagcag agcgtgaccg gggtgcgcgt gcgcgaggcg
     1741 gtgaagggcg tcacagtggc gcaggtgatg gagcaaacgc cgcccgtcgt agcacccact
     1801 tacacgatag cccacctgtt gagccagtac atcctccccc gcaacctgcg cgttgtgccc
     1861 gttgcctacg acggccgcct tgtcggcatc gtcaccctgt ccgacattaa ggacatcccg
     1921 caggaggagt ggggcaccgg gccggctgcc gatgtgatgc ccggcggcga gcggttgcgc
     1981 gtagctcacc cctcggaccg gctcgataag gtcatggaca ccctgatgga aagcgacttc
     2041 gaccagctac ccgtggtaga cgcccgcggc gcgctggtgg gcatgctgac ccgcgcccac
     2101 atcctacgct ggctgaagct acgggaagag ct
//
LOCUS       DASRWZ010000684         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_604520, whole genome shotgun sequence.
ACCESSION   DASRWZ010000684 DASRWZ010000000
VERSION     DASRWZ010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604520"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2133
                     /locus_tag="VFH60_10830"
     CDS             <1..2133
                     /locus_tag="VFH60_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET6314324.1"
                     /translation="AGAGSMRAAPAAAPAEGFAHAAIRQIWERDDAAVASGRATRPWM
                     WGPGPFYTNYEPYAGTPGAGHLVQYFDKGRLEINDPNANRDSPWFVTSGRLVSEMVAG
                     EAEAGGGQTYKVGPANVAVAGDGGPPSATYASFRGLLGGVPLSEGRAVAALLEASGKV
                     SDMSAPPATVTLARREAATGHHWADVFWRFANAPDRPARFDWLYTLGYPITEPYWVQV
                     PVSGKSQAVLVQLFERRSLTFNPANPAALQVEMGNVGRHYFQWRYAQLEAADLDTHYH
                     ARIEVGPRPSRVTRVVEQIELANGTGAPLSNVVLHAPWQHWEGAFRLLSITAGGQQVE
                     TAWREEISLDVQLPAPIAAGGRVTLDLTFELYPRPVGGRTGYDTANDILGLGDMLPSV
                     VPWENGGWSVYPYSELGDLGYYTHGSYTVEITSSGGEKLGAGGTGDTVTYDAGANTWR
                     FEAENVRDVGYVVSPRFINPLTDESMRRQVGDVKVLGYFLPEYREQARQQLSIVVPAL
                     DWLGRTVGYYPFSTYTVAQMGVPKLRSDNYAQEYPTTYFIPTSWLNLGVTPGTWTWYT
                     PVHEVAHQWFYSTVANNQLADPWLDEAMATYVTAEYVRANHPGYYNASYASMTRNATR
                     AMPVSAGIFSGFANENQYSATIYDSGTLMLHRVRSAMGDGPFYAALREYYSTYRFKTV
                     TPGDLLAVLQKHSKADLRPIWDVYLAYY"
BASE COUNT          372 a          690 c          724 g          348 t
ORIGIN      
        1 gccggtgcag ggagcatgcg ggcggccccg gcggcggctc ccgctgaggg cttcgcgcac
       61 gcggccatcc ggcagatttg ggagcgagac gacgcggcgg tggcttcggg cagggcgaca
      121 aggccctgga tgtggggtcc ggggccgttc tacactaact acgaaccgta cgccggcaca
      181 cccggcgcgg gccacctcgt gcagtacttt gacaaggggc ggcttgagat caacgacccc
      241 aacgccaacc gggactcgcc ctggttcgtt accagcggtc gcctggtgag cgagatggta
      301 gcgggcgaag cagaggcagg cggcgggcag acgtacaaag taggcccggc gaatgtggcc
      361 gtggcaggcg acggcggacc gccgtcggct acctacgcca gcttccgggg cctgctaggg
      421 ggagtgccgc tgtctgaggg ccgggcggtg gccgctctgt tggaggcatc gggcaaagtc
      481 tccgacatga gcgctccccc ggccacggtg acgctggccc ggcgggaggc tgccacgggg
      541 caccactggg ccgacgtctt ctggcggttc gccaacgccc ccgaccgtcc cgcccgcttc
      601 gactggctct acacgctcgg ctaccccatc acggagccgt actgggtgca ggtgccggtg
      661 tccggcaagt cgcaggcggt gctggtgcag ctattcgagc ggcgcagcct caccttcaat
      721 cccgcgaacc cggccgcgtt acaggtggag atgggcaacg tggggcggca ctacttccag
      781 tggcggtacg cgcagcttga ggcggcagac ctggacaccc actatcatgc ccgcatcgaa
      841 gttggcccga ggccgagccg cgtcacccgc gtggtagagc agatagagct ggcgaatggc
      901 accggcgcgc cgctcagcaa tgtggtgctg cacgcgccct ggcagcattg ggagggcgct
      961 ttccgcctgc tttcgattac cgccgggggc caacaggtcg agaccgcctg gcgcgaggag
     1021 atcagcctgg acgtgcagct tccggcaccc attgcagcag gtgggcgggt gaccctcgac
     1081 ctcaccttcg agttataccc ccgtcccgtt ggcgggcgca cggggtacga taccgccaac
     1141 gacatcctcg gcttagggga tatgttgccc agcgtggtgc cgtgggagaa cggcggctgg
     1201 tcggtctacc cgtactcgga gcttggcgac ctgggttact acacgcacgg cagctatacg
     1261 gtggagatta cgtcctcggg tggcgagaag ttgggggctg ggggcacggg cgacaccgta
     1321 acttacgacg cgggggcaaa tacgtggagg ttcgaggccg agaacgtgcg cgacgtgggc
     1381 tacgtggtgt cgccgcgctt catcaacccg ctgacggacg agagtatgcg gcggcaggtg
     1441 ggagacgtaa aggtgctggg gtacttcctg ccggagtacc gggagcaggc gcgccagcag
     1501 ctcagcatag tggtgcccgc gctcgactgg ctggggcgca ccgtgggcta ttacccattc
     1561 agcacctaca cggtggccca gatgggcgtg ccaaagctca ggagcgacaa ttacgcgcag
     1621 gagtacccta ccacctattt cataccgacc tcctggctga acctcggcgt aacgcccggc
     1681 acctggacct ggtacacacc cgtgcacgag gtagcccacc agtggttcta ctcgaccgtc
     1741 gcgaacaacc agcttgccga cccctggctc gatgaggcga tggctaccta tgtcactgcc
     1801 gagtacgtgc gcgcaaacca cccaggctac tacaacgcct cctatgcctc catgacgcgc
     1861 aacgccaccc gggctatgcc cgtatccgcc ggcatcttca gcggctttgc caacgagaac
     1921 cagtactcgg ccaccatcta cgacagcggc acgctgatgc tacaccgcgt gcgctcggct
     1981 atgggcgatg gccccttcta cgccgcgctg cgcgagtact acagcaccta ccgcttcaag
     2041 acggtcaccc ccggcgattt gctggccgtg ctacagaagc actcgaaggc ggacttgagg
     2101 ccgatctggg atgtgtatct cgcttactat tgag
//
LOCUS       DASRWZ010000685         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_399301, whole genome shotgun sequence.
ACCESSION   DASRWZ010000685 DASRWZ010000000
VERSION     DASRWZ010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399301"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VFH60_10835"
     CDS             <1..956
                     /locus_tag="VFH60_10835"
                     /inference="COORDINATES: protein motif:HMM:NF037610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HET6314325.1"
                     /translation="HEQLGLGVSLTALYPFYFPFYRSFGYDLTHDAAQYTVKIEQFKQ
                     WRKAAERGKFVPLDVEQLKGDDAGRAGGELEKLDSIYTPYARRNLGNVARDRNWWLHK
                     LIHKEQFAPGYIYHDADGRVAGYIIYHLEDKENWQREMVIHDMRAIDRAAQEAIYGFI
                     YNHDSQAQKVSFWQPVDAGIASQLPDPREAEVKVHSGYMLRLLDVEGAFRQRTFVPEA
                     RGEFSFGLTDEMLPANDGAYHVCVEDGRASVERLANGASAGAGVQLDARALAQLYGGY
                     ASPTRAAAIGQIKVSREADLLGMQAVLYPAGQPIPYMADDF"
     gene            1342..2535
                     /locus_tag="VFH60_10840"
     CDS             1342..2535
                     /locus_tag="VFH60_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET6314326.1"
                     /translation="MQKATQQQTKTHNGRLVLKTIYDQGRISRADVARLTHLTRTTVS
                     DIVADLQEKGLVEEVGMGRSLGGRTPILLSVIDDARHIISVDLANDKLRGAVVNLRNR
                     VIATICLPTNGRAGEEALTLVYELLDRLVYATDRPILGIGIGTPGLVDTTQGVVLSAV
                     NLDWRDLPLGSLLRERYGLPVYVANDSQLAALAQYMFGGEQYGNSLAVVKIGHGVGAG
                     AVLNGQLFQGDGYGAGEIGHIVVVEGGQRCRCGNSGCLETVVSLPAMKAHARALAAQH
                     PKSMLNSIHPSHDIETATINRAFQHGDVAARQLVLETGRYLGMAVAILVGALNVRRVV
                     LMGEVACFGAPLLEIVRQEMLSRALPTLARDTRIDLVDLDPHAVLTGASALLLTNELG
                     LSLTR"
BASE COUNT          510 a          797 c          842 g          493 t
ORIGIN      
        1 tccacgagca gctcggcctg ggcgtatcgc ttaccgccct ttaccccttc tacttccctt
       61 tctaccgctc gttcggttac gacttgacgc acgacgccgc tcagtacacg gtcaagatag
      121 agcagttcaa gcagtggcgg aaggctgccg agaggggcaa attcgtgccg ctcgacgtag
      181 agcagctcaa gggcgacgac gcggggcggg ctgggggtga gttggagaag ctggactcga
      241 tctatactcc gtacgcgcgg cgcaacctgg ggaacgtggc acgcgaccgc aactggtggc
      301 tgcacaagct tattcacaag gagcaattcg cccccggcta catctatcac gacgccgacg
      361 gccgcgtggc gggttacatc atttatcacc tcgaggacaa ggagaactgg cagcgcgaga
      421 tggtcatcca tgacatgcgc gctatcgacc gcgctgcgca ggaggcaatt tacggtttca
      481 tctacaatca cgactcgcag gcgcagaaag ttagcttctg gcagccggtc gatgcgggca
      541 tagcgtcgca gctacccgac ccgcgtgagg ccgaggtaaa ggtgcactcc ggctatatgc
      601 tgcgcctgct ggacgtggag ggcgctttcc ggcaacgcac gttcgtaccc gaagcgcgtg
      661 gagagttcag cttcggcctc accgatgaga tgctgccggc caacgacggt gcgtaccacg
      721 tttgcgtcga agatggcagg gcaagcgtcg agcggctggc gaacggggcg agcgccggtg
      781 ctggggtgca actcgatgcc agggcgctgg cccagttgta cggtggctac gccagcccca
      841 cgcgggccgc ggctataggc cagatcaagg tctctcgtga ggcggacctg cttggcatgc
      901 aagctgtgtt gtaccccgcc gggcagccca taccctatat ggccgacgat ttctagttct
      961 ctagtgataa aatgccctct gtcattctga acgcagtgaa gaatctcagg tgccggttat
     1021 gtgtctacgt cgaacgccgg caccatagga cgtgtcgttc aacgacggcc ctatgtaggc
     1081 ttctctagat tcttcactgc gttcagaatg acagaggccg ctttggtcct gcactcgaaa
     1141 cgcccaagcg tcgtcgggag acgtgcctcc tttattgcag ggcccctgct atggagcacc
     1201 tgagttgaat gcgggtgtct gccactctgt gccattcgca aaaatctcca caccaactat
     1261 tgacaatctg ccaaacccat gctaaacttc gttagtctaa tggattaaca aagtttggct
     1321 cggggtatag acgtgactca gttgcagaaa gccactcagc agcagaccaa gacccataac
     1381 gggcgcctgg tgctcaaaac gatttacgac caggggcgga ttagccgcgc cgacgtggcc
     1441 cgcctcaccc atctcacccg cactaccgtc tccgatatcg tggccgacct ccaggagaag
     1501 ggacttgtgg aggaggttgg catggggcgg tcgctggggg gccgcactcc catcctcctg
     1561 agcgtgattg acgatgcccg gcacatcatc agcgtggacc tggcgaacga taagctacgc
     1621 ggggcggtgg tcaacttgcg taaccgcgtt attgccacca tctgcctgcc gacaaacggc
     1681 cgggcagggg aggaggccct caccctcgtc tacgagttgc tggaccggct ggtgtacgcc
     1741 acagacaggc ccatcctggg tataggtatc ggcacgccgg gcctggtgga caccacgcag
     1801 ggcgtggtgc tgagcgccgt caacctcgac tggcgcgacc tgcccctggg cagcctcctt
     1861 cgcgagcggt atggcctgcc cgtgtacgtc gccaacgaca gccagcttgc ggccctcgcc
     1921 cagtacatgt tcgggggcga gcagtacggc aacagcctgg ccgtagtcaa gataggccac
     1981 ggcgtaggcg cgggggccgt cttgaacggg cagcttttcc agggcgacgg ctatggcgcg
     2041 ggcgaaatcg gccatatcgt ggtagtggag ggggggcagc gttgccggtg cggcaattcg
     2101 ggctgcctgg agacggtggt gagcctcccg gccatgaagg cccacgcccg cgccctggcc
     2161 gcgcaacacc cgaaatctat gttgaacagc atacacccgt cgcacgatat agagactgct
     2221 acgataaacc gcgcgttcca gcatggggat gtcgcggcca ggcaactggt gttggagacc
     2281 gggcgctacc tcggcatggc ggtggctatc ctcgtaggcg cgctgaacgt gcggcgcgtg
     2341 gtgctgatgg gtgaggtagc ctgcttcggc gcgcccctgc tggagatcgt gcgacaggag
     2401 atgctcagtc gcgccctgcc cacgttggcg cgggacaccc ggatcgacct ggtggacctg
     2461 gacccccacg ccgtgctaac cggcgcttcc gccctcctgc tgaccaatga gctggggcta
     2521 agcctgactc gctagctaat cggacccgct ccgggtactc ggcactgagg aatttgcaac
     2581 atatgagcag ctatgacggc tcgccggaat tactaatagg ggtggacgtg gggggcacca
     2641 ag
//
LOCUS       DASRWZ010000686         3427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1014725, whole genome shotgun sequence.
ACCESSION   DASRWZ010000686 DASRWZ010000000
VERSION     DASRWZ010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014725"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(668..2029)
                     /locus_tag="VFH60_10845"
     CDS             complement(668..2029)
                     /locus_tag="VFH60_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6314327.1"
                     /translation="MAYSEYWNPKTETLPRDRLEALQLGKLRRLAGYAYANSPFHKRR
                     FDEAGFHPRQLESLDDLRRIPFMTREEWMQSLVDVPMFGDIISTDPRNAIRYHLTSGT
                     TGRTPIRVLDSTKDWDWISEMWCYGLWGFGIRPEDTVYFAFGYGSFIGFWGAHYACEK
                     MGALVIPGGAQSTESRIKQIVELGVTTVASTPTYALNMWQKAREMGIDLAKDSKVDKL
                     VLSGEPAGSIPAAKRQLEEAWGAKAGDTAGMTELGTIMIFECSHQPGGTHIIEDHYIE
                     EVINPDTGEPVGYGELGERVVTSFGRGFIPVLRYRTKDMVMKVPASTCDCGRTWDIYD
                     GGIRGRWDDMKLIRGTNVYPRAVESIVREHEAIDEFQIYIWRTDRGTDEITVKVEVKP
                     DFEEEWLDLHLKLSKDLAEAHEGLRFNVDREEYGSLPRFELKAKRLVDARPVAEYSQG
                     PKS"
     gene            complement(2056..2661)
                     /locus_tag="VFH60_10850"
     CDS             complement(2056..2661)
                     /locus_tag="VFH60_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314328.1"
                     /translation="MSNLGRASAQNGSLIRRLWCRTVALPVCLMLLSVIAAPATTLAA
                     KRGPSLLTISTIEVTPLSGGPGAVVEIRGVEIYTKVDLYLAVRKTLAARPGQPFPDPN
                     GPTALLGTAEGKGPNGSFATRVTLPATWPSGKAIVEEDMLIGAVRGDVWLDYRDFTFT
                     PPGLPLTGSEGRLDWLSLALLAAGFIAAGAWTLRRADAHRE"
     gene            complement(2654..3280)
                     /locus_tag="VFH60_10855"
     CDS             complement(2654..3280)
                     /locus_tag="VFH60_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314329.1"
                     /translation="MQLVNVTYEGPASDDPRVLDVLPPDLRNLLEQINGFIQFDGGLH
                     VRGACKEPGWHSIGEAMTGEYAFHERYPGVLHTDVPFAQDAIGDQYLLRDGIVHWLYA
                     ETGDVESLRTGLIEFLEAAQANPDTYLGLQLLRQFLAGGGTLQPGELLHVYPPLCTVE
                     AKSGVTVTAVPAGERLKFLAEFAARVRGVSEGGQIEMSGEESGAEHDE"
BASE COUNT          590 a         1122 c         1009 g          706 t
ORIGIN      
        1 aggcagttca tcgtggtact gtccgcaata gctgcaagta aagcccatcc gtcttccctt
       61 tgccctaccg gcgcattcgt taacccgccg gtctcatgca ggcgcttgca tacaccgtta
      121 gcttcgtccg tgtttccagc gagacgtcag cgccggttat gaattcggcc cttctcgcgt
      181 gcgtcgttac cggcgtttgc ttgctttctc gcgcccgcct acttcgcgac tagtcgaatt
      241 gtgcccaggt tatccggggg attgattacc gctcctggtt ccatcccata caacctgcgc
      301 aacgtccttt gctcctcttg acctatacag cgtctcccgc gcatcttctc cctgatgcgc
      361 ctgcgctcat gagcgcgaag gtcctccagg atgagactac gctcgcgctg tacggctgct
      421 aatgtggcga gcaactggta ctctgtcggg tcccttctcc gaaagccgaa ttccgcatga
      481 aatcgttcgc gagcctgaga gaaaggttct cccgtcttcc atgcgagccg aagaatgcac
      541 gagtggagag cactgtaacg gacccccacc ggcaggtttg ggtttagcac cttacgggcg
      601 aaatggttga aagacatacc tggtccctat taacgcacct tcgtagacct gggtgtttgc
      661 gcttgcatca gctcttcggc ccctgtgagt actccgccac cggccgcgca tccacaagtc
      721 gcttcgcctt aagctcaaac ctcgggagcg acccgtactc ctccctgtcc acgttgaagc
      781 gcaagccctc gtgcgcctcc gccaggtcct tcgacagctt gagatgcagg tccagccact
      841 cctcctcgaa gtcgggcttg acctccacct tcaccgttat ttcgtcggtg ccccggtccg
      901 tgcgccagat gtagatttgg aactcgtcta tcgcttcgtg ctcgcgcact atcgactcga
      961 cggcgcgggg gtagacgttg gtgccgcgta tcagcttcat atcgtcccag cggccccgga
     1021 tgccgccgtc gtagatgtcc caggtgcggc cgcagtcgca cgtggaggcg ggcactttca
     1081 tcaccatgtc cttggtgcgg tagcgcagca ccgggatgaa gccgcgcccg aacgaggtca
     1141 ccacccgctc gcctagctcc ccgtagccca caggctcgcc cgtgtcgggg ttgatcactt
     1201 cctcgatgta gtggtcctcg atgatgtggg tgccgccggg ctggtgggag cactcgaaga
     1261 tcatgatcgt gccaagctcg gtcatgcccg ccgtgtcgcc cgccttcgcg ccccacgcct
     1321 cctcaagctg cctcttggcg gcggggatcg acccggcagg ctcgcccgac agcaccagct
     1381 tgtccacctt gctgtctttc gccaggtcta tgcccatctc gcgggccttc tgccacatgt
     1441 tgagcgcgta ggtgggcgtg gaggccacag tagtgacccc aagctccact atctgcttga
     1501 tgcgcgactc ggtggactgt gccccgcctg ggatgacgag cgcgcccatc ttctcgcagg
     1561 cgtagtgcgc cccccagaag ccgatgaacg agccgtagcc gaaggcgaaa tagaccgtgt
     1621 cctcagggcg tatcccgaag ccccacaggc cgtagcacca catctcgctg atccagtccc
     1681 agtccttggt gctgtcgagc acgcggatgg gggtgcgccc ggtggtgccg gaggtgagat
     1741 ggtagcgtat cgcgttgcgc gggtcggtgg agatgatgtc cccgaacatc ggcacgtcca
     1801 ccagcgactg catccactcc tcacgggtca tgaacgggat gcggcgcagg tcgtccagcg
     1861 actctagctg ccgggggtgg aagcccgctt cgtcgaaacg gcgcttgtgg aagggggagt
     1921 tggcgtaagc gtagccggcc aggcggcgca gcttgccgag ttgcagcgcc tccagcctgt
     1981 cgcggggtag cgtctccgtc ttggggttcc agtactctga gtatgccata gtgacctcct
     2041 tgtcaacttt gcgtactact ccctgtgtgc atcggcccgg cgaagtgtcc aggcaccggc
     2101 agcgatgaaa ccggctgcca gcaacgccaa actgagccaa tctagcctgc cttcgctgcc
     2161 cgttagaggc aggccgggcg gtgtgaaggt gaaatctctg tagtccagcc agacgtcacc
     2221 ccggactgcg ccgattaaca tgtcttcttc tacgatggct ttccctgacg gccaggtcgc
     2281 cgggagggtg accctggtgg cgaagctgcc gttaggcccc ttgccttccg ccgtgcccaa
     2341 caaggcagtc ggaccattcg gatcgggaaa gggctggccc ggccgcgcgg caagcgtttt
     2401 ccttacggcg agatacaggt ccactttagt ataaatctcg acgcccctta tctctacaac
     2461 cgcccccggc cctccggaca acggggtaac ttcaattgtc gatatagtaa gcagggaagg
     2521 accccgtttc gcagccagcg tcgtggcagg tgcggctatc acgctcaaca gcataaggca
     2581 gaccggcagg gccacggtcc ggcaccacag gcggcgaatc agcgacccgt tttgggcact
     2641 ggcgcgtccc aaattactca tcgtgctccg cccccgactc ctccccgctc atctctatct
     2701 gtccgccctc cgagacgccc ctcacccgcg ccgcgaactc cgccaggaac ttgagccgct
     2761 cccccgcagg tactgccgtg acggtcacgc ccgatttggc ttccacggtg cacagcggcg
     2821 ggtagacgtg caatagctcc ccaggctgca acgtaccgcc ccccgccagg aactgccgga
     2881 gcagttgcag ccccaggtag gtgtcagggt tcgcctgggc ggcttcgagg aactcgatga
     2941 ggccggtacg gagcgattca acgtcgcccg tctcggcgta gagccagtgc acgatgccgt
     3001 cacggagcag gtactggtcg cctatcgcgt cctgggcaaa aggcacgtct gtatggagca
     3061 cgccggggta acgttcgtga aaggcatatt cccccgtcat agcttctccg atggagtgcc
     3121 agccaggctc tttgcaagcg ccccgcacgt gcagccctcc atcgaactgt atgaagccgt
     3181 tgatctgctc cagcaggttc cgcaggtcgg gaggcagcac gtccagcacc cgagggtcgt
     3241 cgctcgcggg gccttcgtag gtgacgttaa ctaactgcat tggttgccgc ctccttgctt
     3301 gccagcgcta gcgtctatct gtacagtgcc gcccgtgcgg taccagagtg gcccctgtca
     3361 ttctgagcgt agcgaagaat ctaaagaagg tggtacaggg aggctgttga atgatacgta
     3421 ctctggt
//
LOCUS       DASRWZ010000687         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_55397,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000687 DASRWZ010000000
VERSION     DASRWZ010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55397"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VFH60_10860"
     CDS             complement(<1..695)
                     /locus_tag="VFH60_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET6314330.1"
                     /translation="MATEVIAQQPNITEAALNVRGMTCASCVARVERSLKKLGGVEAA
                     SVNLATERALVSYDPLQVGASEMVAAIEKAGYTASPRGEEGLDEAAELQEDRERRARR
                     VEYVRQRDTLIFSVLASIPVVITGMFFMSSPHAIHFFTIFGLVVWYVPGNQFHAIALK
                     NLRHGTADMNTLVSLGTTAASAYSIYILFRDGLLAQTYFEVAAVIITLVLLGRFLETR
                     ARGRTSEAIRKLVG"
     gene            1250..3838
                     /locus_tag="VFH60_10865"
     CDS             1250..3838
                     /locus_tag="VFH60_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6314331.1"
                     /translation="MDVSEDSTQNNDYKFWLTFQQAAVGIALVRPDGRFMRANPELCV
                     LLGYDESELKELSVQNITHRSDVDADAVMTRRMLTGEISSYRAEKRFITRQGRTVWGL
                     QSVSVVRDRVGKPIQFINVIRDISAQKANGAPIPHTHAVEVLQPADLPEDELDAALAT
                     GPLDGTPGQTEPHLASPQDDLRLIELLSALPLGLMIVDTQGETWFANEAARNLLGPGV
                     DPEFAPEAGSEDDNQPHVYSAYKVGTEEVYPLGEAPITRALMGQQAVVSDIEIRRPNG
                     SLPVQVWAGPVYDEQGKVAYAVAAIDELKTRLQVVEQQTQTQALRVGDTVAGLLQLAG
                     EVAAGIGLAPSLEEAMQLCLDRVCEVTGLPVGHMLLAQGDLYARNGAHVHLASAGVWH
                     LQEPERYRALRKATERMVFDMGAELPGQVLTERQAVWTVGPGLIASSPRGKELEACGV
                     TTGFAFPVAADGAMLGVMEFFSIEPLEPDQTLLDVMSLIGANLGNAIARRTATDELER
                     ARGRYELVGQIAGEMLSVHDVEGKYLYTGKAAAALLGYEPADLMGSSLLTYCHPNDVP
                     RLNRLLGRYLAGSDEYSRVKYRFRHKGGDYIWIEMSAQPLVATYGYRREIIAMSKVTQ
                     AELEAAQRAPNGQVITKELKPLQAIDDAQDLDPLTGLYNRETVEDMLTTKLSSRRASS
                     FPVGCLFIDLDDFDELAESYGEETRDEVVRRVAEMLHQTCRDDDFLGRYSGSSFVIVL
                     PNTDAAGTIIVGEKVLRRMRAMEWPGTNVESPVSVSIGGTCLRHGTTLTLPDLLELLG
                     SQLMQAKGSGGDCIIMNARETSRVANSFNPLGGWTQPLRMGLNGQAPGAPAGHAAPPE
                     PGNRSN"
     gene            complement(3767..>4720)
                     /locus_tag="VFH60_10870"
     CDS             complement(3767..>4720)
                     /locus_tag="VFH60_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6314332.1"
                     /translation="IGMSAREFSMLLTLGAIWGASFMFIKLGSEGLQPFALVELRLAL
                     AGVVLLAANAYRKGQFAALLANWRPLTVLGLLNCAVPYVLINWGEHYISSGLAAIYNA
                     CAPLWAAVLLALLPASSERLTGVRLLGLVFGMLGVILVVSGNLSLASEDPLHLVGQGA
                     CLLAALSYAIAGIFARRALVGVPSSVSATGQLVTGAIMLIPLAALQLPDRMPSWVSLG
                     AVVALAVLGTALASMMYYWLIARVGATGALLVTYLLPGFALVWGALFLREQITLTAVL
                     GLCLVLLGIAITSGSGPKLVAAVTRFRRGRMSGGGTGSLPV"
BASE COUNT          920 a         1491 c         1549 g          760 t
ORIGIN      
        1 cctacgagct tgcgtatcgc ctccgacgtg cggcccctgg cccgcgtctc caggaagcgg
       61 ccgagcagca ccagggtgat gatgacggcc gccacctcga agtaggtctg ggcgagcagg
      121 ccgtcgcgga agaggatgta gatggagtag gcggaggcgg cggtggtgcc caggcttacc
      181 agggtgttca tatcggcggt gccgtgccgc aggttcttta gcgcgatggc gtggaactgg
      241 ttgccgggca cgtaccagac caccagtccg aaaatggtga agaaatggat agcgtggggg
      301 ctactcatga agaacatgcc ggtgattacc acgggtatgg aggcgagcac gctgaagatg
      361 agggtgtcgc gctgcctgac gtactccacg cggcgggcgc ggcgctcgcg gtcttcctgt
      421 agctcagcgg cttcatcgag gccctcttcg ccgcggggcg aagcggtata cccggccttt
      481 tcgatggcgg ccaccatctc ggaagcgcct acctgcaacg ggtcgtagct cacgagggca
      541 cgttcggtgg caaggttgac gctggcagct tcgacgcccc caagcttctt gagcgaccgt
      601 tccacgcggg caacgcacga ggcgcatgtc atgccccgca cgtttagtgc ggcttcggtg
      661 atgtttggtt gttgcgcaat cacttcagta gccattttgg ttggcccttg tagcgccggg
      721 cgaagcccaa cgaagtagaa tctgcgacgg caccactctt ctacgcacaa acagcacaag
      781 cagttctagt aaggctatag ctgcaattct accactactg tatgagtttg caagcaccat
      841 cggcttatta gcaccattag agtgggcagc acatcatgtg gcgtgcagca attgcggtcg
      901 cctaaactcc tccttgtcat cctgagccct tcggctacgc tcagggtaaa ctccgcgaag
      961 gatcaggtgg gtaggcgaga gtctacaacg taggggcgat gagggcgata ggaacgctgt
     1021 tgccgcgcaa ggaggagcca aacgcgggcg gctgatcctt cgctacgctc aggatgacat
     1081 gggaacatgg tcgctgcgag tgccgcgctg ccggcacatg ctatagtatt cccttcgtcg
     1141 cggaggggga acaaagttta gtgggaatta ggactaaggt cactccctgt gagggacgct
     1201 catgccgtat tgtaaaggta acctggaagg aggcatacgc ggcctgctta tggatgtttc
     1261 tgaagattcc acacaaaaca acgattataa gttctggctg acctttcagc aggcggctgt
     1321 gggcatcgcc ctcgtgcgcc ccgatggccg cttcatgcgc gccaatcctg agctttgcgt
     1381 gctgctaggc tacgacgaga gtgagcttaa ggagctttcg gtccagaaca ttacccaccg
     1441 cagcgacgtg gacgcggacg ccgtcatgac ccgccgcatg ctcaccgggg agatttccag
     1501 ctaccgggcc gagaagcgct tcattacccg gcagggccgc acggtctggg gcctccagtc
     1561 cgtatccgta gtgcgtgacc gcgtgggtaa gcccatccag ttcatcaacg tgatacgcga
     1621 catcagcgct caaaaggcga acggcgcgcc cattcctcac acccatgccg ttgaagtcct
     1681 gcagcccgcg gacctgcccg aagacgaact cgacgctgcc ctcgcgaccg gccccctgga
     1741 tgggacccca gggcagacag agccgcacct tgcctcgccg caggacgacc tccggctgat
     1801 cgagctgcta tcggcgctgc ccctcggctt gatgatcgtg gacacgcagg gcgagacctg
     1861 gttcgccaac gaagccgccc gcaacctgct agggccgggc gtcgatccgg agttcgcccc
     1921 tgaagccggt tccgaggacg acaatcagcc gcacgtctac tcggcctaca aggtaggcac
     1981 cgaggaagtt taccccctgg gcgaggcccc tattacccgc gccctcatgg gccagcaggc
     2041 ggtggtctcg gacatcgaga ttcgacggcc caacggcagc ctgcccgtgc aggtgtgggc
     2101 ggggccggtg tacgacgagc aggggaaggt cgcctacgcg gtggccgcga ttgacgaact
     2161 caagacgcga ttgcaggtgg tcgagcagca gacgcagacc caggcgctca gggtgggcga
     2221 cacggtggcg gggttgctgc aactcgcagg cgaagtagcg gccggcatcg ggctggcccc
     2281 cagtctcgaa gaggcgatgc agttgtgcct cgaccgggtg tgcgaagtca ccggtctgcc
     2341 ggtgggacat atgttgctgg cccagggcga cctctacgcc cgcaacgggg cgcacgtgca
     2401 ccttgcctct gccggcgtat ggcacttgca ggaaccggag cgctatagag ctcttcgcaa
     2461 ggcgacggag cggatggtct tcgacatggg ggccgagctg ccgggccagg tgctcaccga
     2521 gaggcaggcg gtctggacgg taggcccagg gctgatcgca agctctccgc gaggcaaaga
     2581 gctggaagcc tgcggcgtca ccacagggtt cgctttcccc gtggccgcgg acggtgccat
     2641 gctcggcgtg atggagttct tttccatcga gccgctggaa cccgaccaga ccttgcttga
     2701 tgtgatgtca ctgattgggg ccaacctggg caacgccatc gcccgccgca ccgctaccga
     2761 cgagttggag cgcgcgcggg gccgctacga gttggtgggg cagatcgcgg gcgaaatgct
     2821 ctcggtgcac gacgtggagg gcaagtacct ctacacgggc aaagcggccg ccgcactgct
     2881 cggctacgag cctgccgacc tcatgggcag cagtctgctc acctactgcc accccaacga
     2941 tgtgccccgg ctgaacaggc tgctaggccg ctacctggcg ggcagcgacg aatacagccg
     3001 ggtcaagtac cgcttccggc acaagggcgg cgattacatt tggattgaga tgtcggccca
     3061 gccgctcgtc gcgacttatg gctacaggcg cgagattatc gctatgtcga aggtaacgca
     3121 ggccgagctt gaagccgcgc aacgcgcgcc caacggccag gtgatcacca aggagctgaa
     3181 gccgttacag gccatcgatg acgcgcagga cctcgacccg ctcaccgggc tgtacaatcg
     3241 ggagacggtc gaggacatgc tcactaccaa gctgagcagc cgccgcgcct ctagcttccc
     3301 cgtgggctgc ctcttcatcg acctggacga cttcgatgag ctggccgaga gctacggcga
     3361 ggagacccgc gacgaggtag tcaggcgcgt ggccgagatg ctgcaccaga cctgccgcga
     3421 cgacgacttc ctgggccgct actcgggcag cagcttcgtg atcgtgctgc ccaacaccga
     3481 cgcggcgggc acgatcatcg tgggcgagaa ggtgttgcgc aggatgcggg ctatggagtg
     3541 gccgggcacg aacgtcgaga gcccggtcag cgtgagcatc ggcggcacct gcttgcggca
     3601 cggcacgacg ctcactctgc cggacctttt ggaactgctc ggctctcaac tgatgcaggc
     3661 caagggttcg ggcggcgact gcatcatcat gaacgcccgc gagacgagcc gggtcgccaa
     3721 cagcttcaac ccgctgggcg gctggacgca gcccctgcgt atgggcctaa acgggcaggc
     3781 tcccggtgcc cccgccggac atgcggcccc gcctgaaccg ggtaaccgca gcaactagct
     3841 tcggcccgct cccggacgta atcgcgatac ccagcagcac gaggcacagc cccagcacgg
     3901 cggtgagggt tatctgctcg cggaggaaca gagcgcccca caccagggcg aagccgggca
     3961 gcaggtaagt caccagcagc gcgcctgtgg cccctacccg cgcaatcagc cagtagtaca
     4021 tcatcgaagc cagggcggta cccagcaccg ccagcgcgac cacggccccc aacgacaccc
     4081 acgaaggcat cctgtcgggc agttgcaggg cggccagggg tataagcatg atcgcgcctg
     4141 tgacaagctg cccggtcgcc gaaacggacg aaggcacgcc caccagcgcg cgcctggcga
     4201 agatgcccgc tatagcgtag gagagcgcgg ccagtaagca ggccccctgc cccaccaggt
     4261 gcagcgggtc ctcgctcgcc agcgacaggt tgccgctcac caccagtatc acgccgagca
     4321 tgccgaacac caggccgagt aggcgcaccc cggtcagccg ctcggaggag gcgggcaaga
     4381 gggccaggag cacggcggcc cagagaggag cgcaggcgtt gtagatggcg gccagcccgc
     4441 tgctgatgta gtgctcgccc cagttgatga gtacgtaggg caccgcgcag ttgagcaggc
     4501 ccaatacggt gagcggacgc cagttggcca gcaaggccgc gaactgcccc ttgcggtagg
     4561 cattggcggc cagcagcacc acacccgcca gcgccaaccg tagctccacg agggcgaaag
     4621 gctgcaagcc ctctgagccg agcttgatga acatgaagga cgcgccccag atcgcgccca
     4681 gagtcagcag catcgagaac tcacgcgccg acatcccgat
//
LOCUS       DASRWZ010000688         4093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_193327, whole genome shotgun sequence.
ACCESSION   DASRWZ010000688 DASRWZ010000000
VERSION     DASRWZ010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193327"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFH60_10875"
     CDS             complement(<1..432)
                     /locus_tag="VFH60_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6314333.1"
                     /translation="MTRVVKTKIEIEGRVHEETVVVERDEPAAWEAGREFSQVGKSAN
                     RVDGRDRVTGAARYTYDMHPAGLLYAAVLRCPHPHARIVALDTSEAERLPGVRGVLSR
                     NNAPDIPWYGGASKLFDEELRFAGEEVAVVAADDLDTARDGL"
     gene            complement(477..992)
                     /locus_tag="VFH60_10880"
     CDS             complement(477..992)
                     /locus_tag="VFH60_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6314334.1"
                     /translation="MRPSHDESDHTPVAGTTSRNIELTVNGETYQATVPSRRTLLDLL
                     RDQLALTGTKKVCDMGHCGACTVLRDGKAVLSCLTLATACDGADIRTVEGLAAPDGAL
                     SPLQQAFIDADAFQCGFCTPGQLMSATALLADNPTPSPQEIRAYMAGNLCRCGAYSGI
                     VDAIERASRQG"
     gene            complement(1009..2130)
                     /locus_tag="VFH60_10885"
     CDS             complement(1009..2130)
                     /locus_tag="VFH60_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314335.1"
                     /translation="MSVMWHARRKSTVMVALLALLAAVVAGCAAPTTISEGGSAGKYD
                     VRVRMDPVHLNPPALGTLSFEISQMTDQNTKQAITEFQPVFDIGDLMHTIIIHKDMTS
                     FRHSFTDRLVLGSASVPVGFPELGTYYTWTYFKPVGAEVQVFETTVQAGTEPATQPQD
                     VDVRPKVSYGLRVELLNGGEALHVGEPVQLAFRVTQRGYPVRDLQAYFGAPGHLWIVQ
                     VPEGGAHGAEASAEEHAPKLGHELGSAQSYVKAVQTPEEGQSETTDSGFPNSGNLVGS
                     DMVGGGRAPTPPAPTFQPQVATAVASITAQPVSTLLPVQQTPQAAVIGTQVVEPGVGY
                     GPEVAFTHTFEHAGLHKMWLELRHGREIIVTDFVVRVEE"
     gene            complement(2240..>4093)
                     /locus_tag="VFH60_10890"
     CDS             complement(2240..>4093)
                     /locus_tag="VFH60_10890"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6314336.1"
                     /translation="AHIAAEDYESSYQINLEDNVIPGILGRVCARPCEPACRRGRLDE
                     PIAICWLKRVAADYRGPWTPPPSAPRTSEKTVAVIGAGPTGIAAARDLAKLGYGVTVY
                     EALPVAGGMLTAGIPEWRLPRDLCKREIDEYLAALGVEIKLNTPIGFGTYPGAEPANE
                     NAIPLDELMGMHDAVLIAAGTQHPQEMEIAGEDYETLTGLFPGLWFMERVNLHQQVDV
                     GKRVAVIGGGFTAMDCSRSSLRMGAEKVYVLYRRSRNEMLVYEEEAREAEIEGVDFRF
                     LVSPLEILHENGKVVGLKCIRNRLGDPDASGRRSPVPIPGSEFVLELDSVIAATGQNS
                     DTAWVPETLGMKVNRRGRPETDKLNWSTSVPGLFAAGDFVEGARTIIACVADGQKAAI
                     AIDQFLKGADTKADTVQLQADFALVPYLDYVSDYHLDVTRELMEWAESETVREETAPE
                     ATVRMSWQPISIWTADTMQRRLVDGDDYDAVPRQHMPMLPLPQRFNLETEVELGFHKH
                     EAFQEAKRCLQCQLNIFLDGDNCILCNACVEVCPTNVIHMADLDLIDSVNGSEEEPRL
                     VEARSWKHGAAMIMDEYLCIRCGLCSQICPTNCITMQHYEPKLVRGELLVP"
BASE COUNT          675 a         1336 c         1298 g          784 t
ORIGIN      
        1 gagcccgtca cgtgccgtat ccaggtcgtc ggcggccacc accgccacct cttccccggc
       61 gaacctcagt tcctcatcga atagcttgct tgctcctccg taccagggta tgtcgggtgc
      121 gttgttcctg gaaagcaccc ctcgcactcc cggcaggcgc tcggcctcgc tcgtgtccaa
      181 cgcgacaatg cgggcgtgcg ggtgcgggca gcgtagcact gcggcataga gcaagcccgc
      241 ggggtgcatg tcatacgtgt acctggcggc tccggtcacc ctgtcgcgcc cgtctacgcg
      301 gttggccgac ttgccaacct gcgaaaactc gcgcccggcc tcccatgccg ccggttcgtc
      361 ccgctcgacc accaccgtct cttcgtggac gcggccttct atctcaatct tggtcttgac
      421 gacgcgcgtc atgggcctct cctaactatt gggtggtagc gcggggcagc aggcgattag
      481 ccctgccgcg acgcccgctc gatggcgtcc acgatgccgc tgtaggcacc gcaccggcaa
      541 aggttacccg ccatgtacgc ccgaatctcc tgggggcttg gcgtggggtt atcggcgagc
      601 agcgccgtgg cggacatcaa ttgcccaggc gtgcaaaagc cgcactggaa cgcatcggca
      661 tcgatgaacg cctgctgaag cggtgagagc gcgccgtcgg gtgcggccag gccctcaacc
      721 gtgcggatgt cggccccgtc gcaggccgtg gcaagcgtga ggcaggacag taccgctttg
      781 ccatcccgca ggaccgtgca ggccccacaa tgccccatgt cgcacacctt cttggtgcct
      841 gtgagggcta gctggtcgcg cagcaggtcg agcagcgtgc gccgtgaagg cacggtggcc
      901 tggtatgtct cgccatttac ggtcagctcg atgtttcggg aggttgtgcc agcgaccggg
      961 gtgtggtcgg attcatcatg tgagggccgc atagtctcct tcgcgcttct actcctcaac
     1021 gcgcaccacg aagtcggtaa cgatgatctc gcggccgtgc cgtagctcaa gccacatctt
     1081 gtgcaggccg gcatgctcga aggtatgggt aaaggctacc tcaggcccat aaccgacgcc
     1141 cggttccaca acctgcgtgc cgattacggc ggcctgcggt gtttgctgca ccggcaggag
     1201 ggtcgatacg ggttgcgcgg tgatgctggc gacggcggtg gctacttgcg gttggaaggt
     1261 cggcgcggga ggcgtaggtg ccctgccgcc gccaaccatg tcgctcccca ccaggttgcc
     1321 gctgttcggg aaacccgagt cggtggtttc gctctgcccc tcttcgggcg tctgtacggc
     1381 tttcacgtat gactgcgcgc tacccagttc gtggcccaac ttgggtgcgt gctcctccgc
     1441 ggacgcctca gcgccatgcg cgccgccttc aggcacctgt acgatccaca ggtggccggg
     1501 cgcgccgaag taggcctgca agtcgcgcac cgggtagcca cgctgcgtca ctcggaacgc
     1561 aagctgcaca ggctcgccaa cgtgtaacgc ctcgccacca ttcagcagtt cgacgcgcag
     1621 gccgtaggag accttcggcc tcacgtccac gtcctgcggc tgcgtggcag gctcggtccc
     1681 cgcctgtacg gtcgtctcga agacctgtac ctcggcccca acaggcttga agtaggtcca
     1741 ggtatagtag gtgcccagct cagggaagcc caccgggacg ctggccgaac cgagcacgag
     1801 ccggtcggtg aagctatgcc ggaaggaggt catgtccttg tggatgatga tggtgtgcat
     1861 caggtcgccg atgtcgaaca cgggctggaa ctcggtaatg gcctgcttcg tgttctggtc
     1921 ggtcatctgg cttatctcaa aggagagggt gcccagcgcc ggggggttca ggtgcaccgg
     1981 gtccatcctg acgcgcacgt cgtacttgcc cgcgctgccg ccttccgaga tggtcgtagg
     2041 ggcggcgcag cccgcgacaa ccgccgccag gagggccaaa agtgccacca taaccgtaga
     2101 cttacgccgg gcgtgccaca ttacagacac aacgcttcct cctggtaaaa gtgcgcgagt
     2161 acaaccgtgg gattatatca cagacaggga cgatggaccg ctaaattcga aattcgaaat
     2221 cccaaattcg aatttcctac tacggtacca acaattctcc cctgaccagc ttcggctcgt
     2281 agtgctgcat ggtgatgcag tttgtggggc aaatctggga gcagaggccg caccggatgc
     2341 acaggtactc gtccatgatc atggccgcgc cgtgtttcca gctacgcgcc tccaccagcc
     2401 gcggctcctc ctcgctgccg ttgaccgagt cgatgaggtc gaggtcggcc atgtggatca
     2461 cgttggtggg gcacacttcc acgcaggcgt tgcacagaat gcagttgtcc ccgtcgagga
     2521 agatgttcaa ctggcattgc aggcaccgct tggcctcctg gaacgcctcg tgtttgtgga
     2581 agcctagctc cacctcggtc tccaggttga agcgttgggg gaggggcagc atcggcatgt
     2641 gctgcctggg taccgcatcg tagtcgtcgc cgtcaaccag gcggcgctgc atcgtatcgg
     2701 ccgtccagat cgagataggc tgccaggaca tcctgaccgt ggcttcgggg gcggtctcct
     2761 cgcgcaccgt ctcgctctcc gcccattcca tcaactccct ggttacgtcc aggtggtagt
     2821 cggacacgta gtcgaggtaa ggcaccaggg cgaaatccgc ctgcaactgc accgtgtcgg
     2881 ccttcgtgtc cgcgcccttg aggaattggt cgatagcgat ggccgccttc tgcccgtcag
     2941 ccacgcaagc gatgatagtg cgggcaccct ccacgaagtc gcctgccgcg aagaggccgg
     3001 gcaccgaggt gctccagttg agcttgtcgg tctcgggcct gccgcgcctg ttgactttca
     3061 taccgagggt ttcgggcacc caggccgtgt cgctgttctg cccggtggca gcgattacgc
     3121 tgtcaagctc cagcacgaac tccgagccgg ggataggcac gggcgagcgg cggccactgg
     3181 cgtcggggtc tcccagccgg ttcctgatgc acttcagacc gactaccttg ccgttttcgt
     3241 gcaatatttc gagcggcgac accaggaagc ggaagtctac accttctatc tcggcctccc
     3301 gtgcctcttc ctcgtagacg agcatttcgt tgcgcgaccg ccggtagagc acgtacacct
     3361 tctccgcgcc cattcgcaag gacgagcggg agcagtccat agccgtgaag ccaccgccaa
     3421 ttacggccac tcgcttgccg acgtccacct gctggtgcag gttcacgcgc tccatgaacc
     3481 acaggccggg aaacaggccc gtcagcgtct cgtagtcctc gcccgcgatc tccatctcct
     3541 gcgggtgctg cgtgcccgcc gcgattagca ccgcgtcgtg catacccatc agttcatcca
     3601 aggggatggc gttctcgttg gcaggctcag cgccggggta ggtgccgaag ccaatcgggg
     3661 tgttgagctt gatttcgacg cccagggccg ccaggtactc gtcgatctcg cgcttgcaca
     3721 ggtcgcgtgg caagcgccac tccggtatac cggccgtaag catgccgccc gccacgggca
     3781 gcgcctcgta caccgtcaca ccatagccga gctttgccag gtcgcgggcc gccgcgatgc
     3841 cggtaggccc cgcgcctatg accgccacgg tcttctcgct ggtgcggggt gccgatggtg
     3901 gcggggtcca ggggcctcga tagtcggcag cgacgcgctt gagccagcag atagcgattg
     3961 gctcatcgag gcggccgcgg cggcaggcag gctcgcacgg gcgggcgcac accctcccca
     4021 ggatgccggg gatgacgttg tcctccaggt ttatctggta agacgactcg tagtcctcgg
     4081 ctgcgatgtg ggc
//
LOCUS       DASRWZ010000689         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1083821, whole genome shotgun sequence.
ACCESSION   DASRWZ010000689 DASRWZ010000000
VERSION     DASRWZ010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083821"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..904
                     /locus_tag="VFH60_10895"
     CDS             113..904
                     /locus_tag="VFH60_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET6314337.1"
                     /translation="MAANSTGQPQPGYIVQAEDVHKRFGHVEVLRGIDLLVKPGETVV
                     LIGPSGSGKTTFLRCINHLEKINSGCIYVGGQLIGYRKQGDRLVEDSERNIAAQRARI
                     GMVFQRFNLFAHMTALENIIEAPMRVKKVPKAEAIEKARALLAKVGLSEKDDSYPSQL
                     SGGQQQRVAIARALAMDPMLMLFDEPTSALDPELVGEVLKVMEQLAMEGMSMVVVTHE
                     MSFARDVADRVVFMDMGRVVEEGSPSEMFDNPQHERTLTFLKRIL"
     gene            1144..>3476
                     /gene="uvrA"
                     /locus_tag="VFH60_10900"
     CDS             1144..>3476
                     /gene="uvrA"
                     /locus_tag="VFH60_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006381085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET6314338.1"
                     /translation="MSIPKNKLVVLTGLSGSGKSTLAFDTLHNEAIRQYLESVGMVTY
                     QQKPSVERIEGLSPSISIDQHLTNHSPRSTVGTVTEVFTYLRVLFARIGQQPCPACGQ
                     AVPPSHNVTGLELWDADSAGDLGGSAVDTYPCPHCGAAVPELGMAHFSFNKPEGACPT
                     CTGLGVVHAANLSLLIDPARSIPGGGVVRWVSQEITRHLETLQLAAKHFGFAFDPSAP
                     IGEFTQAQRDLLLYGVHSREFRRHYPHIEPPETVNQGRFEGVVTALMRRYQEHAQDAE
                     YRERLEQVMVTQTCPDCEGHRLRLESRQVRVAGSSIIDLSQIPFTALHTWIEGLRGIV
                     PTSEADVVQPIIDDLSERIRRLVKVGAGYLTLERSSPTLSQGEAQRLRLAALLSSGLT
                     GVLYVFDEPTIGLHQRDTGRLIDVLRQLRDLGNTVLVVEHDLEVVRAADHVIDFGPGA
                     GQHGGRIVAEGTPAEIAASTASITGDYLSGRVVMAGSRGRPGNGKRLVIRGAREHNLK
                     NVTVGVPLGTLTAVAGVSGSGKSSLMLDILDRAARQRFNGATEAPGKHDSIEGWEHLD
                     KIISIDQAAIGRTSRSNAATYTDAFTPIRQVFAETPEARGRQLTPGHFSFNVPGGHCE
                     RCKGAGTLTVNMHFLPDVLVRCPACKGRRFKQEVLAVRYRGYDIAEVLDLTIEEALAV
                     FQDIAAVAARLSLMSEVGLGYLQLGQPANTLSGGEAQRIKLAKELARRTTGRTLYLLD
                     EPTTGLHPADVVRLLDLLQRLVDGGNTVVVIEHHIDV"
BASE COUNT          674 a         1061 c         1131 g          610 t
ORIGIN      
        1 aaggcgcaaa ggtcgcatta tagaggatga gtggtgctgt cagtgccact cagtcctcag
       61 tcctcagtcc tcagtcctca tcactcatta ctctcctcat ggagccaagc gaatggcagc
      121 aaacagcaca gggcagccac agccggggta catcgtgcag gccgaggacg tgcacaagcg
      181 gttcgggcac gtggaggtgt tgcgcggcat cgacctgctg gtgaagccgg gcgagacggt
      241 ggtgctcatc gggccgtcgg ggtcgggtaa gacgaccttc ctgcgctgca tcaaccacct
      301 ggagaagatc aacagcggct gcatctacgt gggcgggcag ctaatcggct accgcaagca
      361 gggcgaccgc ctggtggagg acagcgagcg caacatcgcg gcccagcgcg cccgcatcgg
      421 catggtcttc cagcggttca acctgttcgc ccacatgacc gcgctggaga acatcatcga
      481 ggcccccatg cgcgtcaaga aggtacccaa agcggaggcc atcgagaagg ccagggcgct
      541 gctcgccaag gtggggctga gcgagaagga cgacagctac ccgagccagc tatcgggcgg
      601 gcagcagcag cgcgtggcaa tcgcccgcgc gctggcgatg gacccgatgc tcatgctgtt
      661 cgacgagcct acgtcggcgc tcgaccctga gcttgtgggc gaggtgctca aggtgatgga
      721 gcagctcgcg atggagggca tgagcatggt ggtggtcacc cacgagatga gcttcgcccg
      781 cgacgtggca gaccgcgtgg tgtttatgga catgggccgg gtggtagagg aaggttcccc
      841 cagtgagatg ttcgacaacc cccagcacga gcgcaccctg acgttcctca agaggattct
      901 ttaaggggtg gacgatggac gacggacggt ggaggggaag gaactgagca ggtgcgaagc
      961 atgagaagtt gaatgttctg taagggtttg atatgatggt ttctcgcttg agaaaagtcc
     1021 cacgggtaca tccttaagtg ggttttttct tttaagacgc aatggtagga ggttgacgca
     1081 cagagcatgc aagacacgat aaggataaaa ggcgcgcgtc ttcacaatct gaagaatatc
     1141 aatgtgagta ttcccaagaa caagctggtc gtgctgaccg ggctgtccgg ttcaggcaaa
     1201 tcgaccctgg cgtttgatac cctgcataac gaggcgattc gccagtacct tgaatcggtc
     1261 ggtatggtga cgtaccagca gaaaccgagc gtcgagcgca tcgaggggct atccccttcc
     1321 atcagcatcg accagcattt gaccaatcac agcccgcggt ccacggtggg caccgtcacg
     1381 gaagtgttca cctacctgcg cgtgctgttc gcccggatag gccagcagcc atgccccgcc
     1441 tgcggccagg ctgtcccacc gtcccacaac gtcaccgggc ttgagctgtg ggatgcagac
     1501 tctgccggcg atcttggcgg ctctgcggtg gacacctatc cctgcccaca ctgcggcgcg
     1561 gccgtacccg agctggggat ggcccacttt tccttcaaca agcccgaagg ggcgtgccca
     1621 acctgcaccg gcctgggcgt cgtgcacgcg gccaacctgt ccctgctgat cgacccggcg
     1681 cgcagcatcc ctggcggtgg ggtcgtgcgt tgggtgtcgc aggagatcac gcggcatctc
     1741 gaaacgctgc aactggccgc caagcacttc ggttttgctt tcgacccgtc cgcgcccatt
     1801 ggagaattca cgcaggcgca acgcgacctg ctgctgtacg gcgttcacag ccgggagttt
     1861 cgacgccact atccgcacat cgaaccgccg gagaccgtca accaggggcg atttgagggc
     1921 gtggtcaccg ccctgatgcg ccgctaccag gaacacgcgc aggatgcgga gtatcgcgag
     1981 cggctggaac aggtgatggt gacgcagacc tgcccggact gtgagggcca ccgcctgcgc
     2041 ctggaaagcc gccaggtgag ggttgccggg tcgagcatta tcgacctgtc gcaaataccc
     2101 ttcaccgccc tccacacgtg gatagaagga ctgcggggaa tcgtacccac gagcgaagcg
     2161 gacgtcgtcc agcctattat cgatgacctg agcgaacgca tccgccgcct ggtgaaagtg
     2221 ggagcgggct atttgaccct tgagcgttcc tcgcctaccc tctcacaggg tgaagcccag
     2281 cggttgcgcc tggccgccct cctgagctcc ggcctgacag gtgtgctgta tgtgttcgat
     2341 gaaccgacga ttggcctgca ccagcgcgat acggggcggc tgatcgacgt gctgcgccaa
     2401 ctgcgcgacc tgggaaacac ggtgctggtg gtcgagcacg acctggaagt ggtccgcgcg
     2461 gcggaccacg tgatcgattt cggcccaggc gcgggccaac acggcgggcg catcgtcgcg
     2521 gagggcacgc ccgccgagat cgcggctagc acggcgtcga tcactggcga ttacctttcg
     2581 ggccgagttg tcatggcagg ctcccggggg cggcccggca acggcaagcg cctcgtcatc
     2641 agaggggcgc gcgagcataa cctgaaaaat gtcaccgtcg gggtgccgct agggaccctc
     2701 accgccgtcg cgggggtctc cggttccggc aagtcctcgt tgatgctcga cattctagac
     2761 cgggcggccc gccagcgctt caacggcgcg accgaagctc ccggcaagca cgacagtata
     2821 gagggctggg agcacctgga caagatcatc tcgattgacc aggcggctat cgggcgcacg
     2881 tcccgctcca acgcggcgac ctatacggat gcctttacac ctatccgcca ggtgttcgcc
     2941 gaaacgcccg aggcgcgcgg ccgccagtta acgccgggac acttttcgtt caacgttccc
     3001 ggtgggcact gcgagcgctg caaaggcgcc ggtacgctca cggtgaatat gcactttctg
     3061 ccggacgtcc tggtgcgctg tccggcttgc aaaggacggc gtttcaaaca ggaagtgctc
     3121 gccgttcgct atcgcggcta cgacatcgcc gaggtgctcg acctgacgat tgaggaggcg
     3181 ctggcggtat tccaggacat agcagcggtg gcggcgcgac tatccctcat gtccgaggtc
     3241 gggttggggt acctgcaact ggggcagcct gccaatacgc tgtcgggtgg ggaagcgcaa
     3301 cgcatcaagc tggccaaaga actggcgcgc cgcacgacgg gtcgcacgct ttacctgctg
     3361 gacgagccca ccaccggcct gcaccccgcc gacgtcgtcc ggctcctcga cctgttgcaa
     3421 cggttggtgg atggcggcaa caccgtcgtg gttattgagc atcacatcga cgtcat
//
LOCUS       DASRWZ010000690         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1357739, whole genome shotgun sequence.
ACCESSION   DASRWZ010000690 DASRWZ010000000
VERSION     DASRWZ010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357739"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1039)
                     /locus_tag="VFH60_10905"
     CDS             complement(230..1039)
                     /locus_tag="VFH60_10905"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET6314339.1"
                     /translation="MARYKATLLSSRPKFNVAMITALLTALCLGAIAMPSASAASAQA
                     QENVQAQYAPSSVVFNTAPGSYGGLWIGEMSGGGNVRSGPGIANARIKSWGAGRRVLL
                     YQGVTDASGGLWYRVSEAPEQPMYVHSSLVRRVAAVKFEAGKFRGKWVNINLTQQVVT
                     AYEGGTPLRVTLASTGTKKYPTEVGVWKIFYKLPKQDMDGGSRAAGDYYNLKDVPYPQ
                     YFHKSGEGLHGTYWHDNFGRPMSHGCVNLSTPMASWFYGWAPIGTTVWSHY"
     gene            1627..3438
                     /locus_tag="VFH60_10910"
     CDS             1627..3438
                     /locus_tag="VFH60_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314340.1"
                     /translation="MIVYGDPQYQEGLPNLVEQLRQRIEIYLDASLNKDAGERLQQLR
                     SALILAGQLEQAAYDASDQPGAQGYNSSLHDATTHIGAAFYAAWAEQNGAQPTGGLDR
                     EASLAGALAHLAGAVELPEAPVAVKMPEGFAFYTLYPEQYCAAALRWLDAHQAVEGKR
                     AVVVGIRSIGTTLAAVVAATLTAHGRHATSLTLRPTGHPFRREAHISPEQVEGAEWGL
                     VVDEGPGLSGSSMSAVGQALVRAGLSRHNVSFFSSHANGPGSATSDEVRAWWSGTPTY
                     VVPLGEMRFGGLSLPESLAAWLTARDDSDPVTQIEDASGGRWREWLYEDGDKWPPACA
                     PFERTKYLCTTRSGRRVLFKFEGLCGAPGTAATTAELVQGQLADRATSGWTAAPVGVV
                     NGFVAGEWVEGKPLGQGDCDPAVLRHVGRYVAAVSGPALTVEQHETGSKRLAEMLYWN
                     AWEALGEAMAERTRPWAERLQAVGHGSRSYGDGRMAPHEWIRTNEGQLVKADSAGHAW
                     DHTCIGPQPVAWDLAGAIVEWGLNDEAAGVLSQAFYAAVGERITEDALTAYRLAYAAF
                     RAGQCHLCANMSAHDPTEQARLGAAYTRYKDMLAALL"
     gene            complement(3483..>3919)
                     /locus_tag="VFH60_10915"
     CDS             complement(3483..>3919)
                     /locus_tag="VFH60_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314341.1"
                     /translation="DEEFPEKLPHAVSRHVFDLGIVSRIIVGAVAAVAALLVLSPRTG
                     FGLVATALIAGSAGTSILRSLQDRITAALAVGEVNQARENGAKAEEVIDQVRARLEEA
                     QARASASLSAESSAAARGVPALDDIQSLLGEAKGLARSISKR"
BASE COUNT          709 a         1209 c         1285 g          716 t
ORIGIN      
        1 gaaaggtcta aacgccaccc cgcgaggaga cgaaaacgca aagacgcaaa gacgcaaagg
       61 acgcgcaaag agaataaagg gttagatagt gcaaaaggac cagagtacgg atttagtacc
      121 ctggtccttt tgcgcagaac atcaatcctt aatctctgcg cgtcctttgc gtctttgcgt
      181 ctttgcgttt tcgtctcctc gcggggtggc gtgagtagga tgccggtacc tagtagtgag
      241 accagacggt ggtgccgatg ggtgcccagc cgtagaacca gcttgccatg ggggtgctca
      301 ggttgacgca gccgtggctc atgggccgcc cgaagttgtc gtgccagtag gtgccgtgca
      361 gcccttcgcc cgacttgtgg aagtactgcg ggtaaggcac gtccttgagg ttgtagtagt
      421 cccctgcggc ccggctgccg ccgtccatgt cctgcttggg cagcttgtag aagatcttcc
      481 acacgccgac ttcggtgggg tacttcttgg taccggtgga ggcgagcgtc acgcggagcg
      541 gcgtgccgcc ctcgtaggcg gtgactacct gctgggtgag gttgatgttg acccacttgc
      601 cccggaactt gccggcctcg aacttgaccg cggccacccg gcgcaccagg ctggagtgca
      661 cgtacatcgg ctgctccggc gcttcggaca cgcggtacca gaggccgccg ctcgcatcgg
      721 tcacgccctg gtagagcagc acgcgccgcc ccgcgcccca cgacttgatc cgggcgttcg
      781 ctatgccggg gccgctcctg acgttacccc cgccgctcat ctcgcctatc cacagcccgc
      841 cgtagctgcc cggcgcggtg ttgaacacca cggacgacgg ggcgtactgg gcctgcacgt
      901 tctcctgcgc ctgcgcgctc gccgctgagg cggatggcat ggcgatagct cccaggcaca
      961 aagccgtgag gagcgcggtt atcattgcga cgttgaattt gggcctgctc gataggaggg
     1021 tagccttata tcttgccatc tcgcacctcg acacaattat tctatgatgc ttgttgatcg
     1081 ttgtgcctag ccgcgcaccg aaggtacctg catgatactt gccatcgttt tcaccgggag
     1141 gacgcaactt gccggtcgag ccgcgaccgg gcattgttgc tctatgaagc gaatgatgtt
     1201 ggagagggtg cgcgtgcgtc tgcatatact taacgcacgc accggcgact cggtacgggt
     1261 ttggcctggc agtttcgtaa ataattgtta agtggtgctt cgatggtcag ggctgatgga
     1321 agctatctca aacgtaacta tgtggaatac gagcacatgg ccccgcctat gtcatttcga
     1381 gtgtagcgag aaatctcgtc cttcagcacc caggtgccac ctttcttacg agacagagtc
     1441 ccttaagaaa aaggagacat ccgggtgaag gacgagattt ctcgctgcgc tggaaatgac
     1501 aagggcgggg cttaggtact tacgttcgac agtttcgttc tagagttagc ttctaataac
     1561 acctatcccc gacaggcttc ctccacttac atactggctc ggctcttgca tgcctaagca
     1621 gccgccatga tagtctacgg cgacccacag taccaggaag gcctgcctaa cctcgtggag
     1681 cagttgcggc aacgcataga aatctacctg gatgcctcgc tcaataagga cgcgggggag
     1741 aggttgcagc agctacgctc cgcgctcatc ctggccgggc agttggagca ggccgcgtat
     1801 gatgcttcag accagccggg agcgcagggc tacaacagca gcttgcacga tgccactacg
     1861 catattgggg cggcattcta cgccgcctgg gccgagcaga acggcgcgca gcccacagga
     1921 gggttggacc gggaggcgag tctcgccggc gccctggccc atctcgcggg agcggttgag
     1981 ctgccggagg cacctgtagc ggtcaagatg ccggagggtt tcgcgttcta caccctctac
     2041 ccggagcagt actgcgccgc cgccctccgg tggctggacg cgcaccaggc ggttgagggt
     2101 aagagggcgg ttgtggtcgg gatacgcagc atcggcacga ccctggccgc tgttgtcgcc
     2161 gccacgctga cggcccacgg ccggcacgcg acgagcctta ccttgcgccc caccgggcac
     2221 cccttccggc gcgaagccca catctcccct gaacaggttg agggtgcgga gtggggcctc
     2281 gtggtggacg aggggccggg gctgtcgggt tcgtccatgt cggctgtggg gcaggcgctg
     2341 gtgcgagcgg gcctctcgcg ccacaacgtt agcttcttct cctcgcacgc aaacggaccg
     2401 ggcagcgcca catcggatga ggtccgcgcc tggtggtcgg gcacgccaac gtacgttgtg
     2461 cctctcggtg agatgcgctt cggggggcta tctctgcccg agtccctggc ggcatggctg
     2521 actgcccgcg acgacagcga ccctgtaacg cagatcgagg acgcgagcgg gggccggtgg
     2581 cgcgaatggc tgtacgagga cggcgacaag tggccgcccg cgtgcgcgcc attcgagcgg
     2641 accaagtacc tctgcacgac acggagcggc aggcgcgtgc tgttcaagtt cgaggggctg
     2701 tgcggcgcgc ccggcacggc ggctaccacg gcagaattgg tccaggggca attggcggac
     2761 cgcgcaactt ccgggtggac ggccgcgccg gtgggggtgg taaacggctt cgtcgccggc
     2821 gagtgggtgg agggtaagcc gctcggccag ggggactgcg acccggcggt gctccggcac
     2881 gtggggcggt acgtcgcggc ggtgtccggc cccgcgctca cggtggagca gcacgagacg
     2941 ggcagcaaga ggctggccga aatgctttac tggaacgcgt gggaagcctt aggagaggca
     3001 atggcggagc gcacccgacc ctgggccgag cgcctgcagg ccgtagggca cggctcccgc
     3061 agctacggcg acggccgcat ggcaccccac gagtggatac gcacgaacga gggccaactg
     3121 gtgaaggcag acagcgccgg gcacgcctgg gaccacacct gcataggtcc gcagccggtg
     3181 gcctgggacc tcgcgggggc aatcgtggag tggggcctca acgatgaagc cgcaggtgtt
     3241 ttatcgcaag ccttctacgc ggcggtaggc gaacgcatta ccgaagatgc ccttaccgcc
     3301 taccgcctgg cctacgccgc cttccgggcc ggacaatgcc atctctgcgc gaacatgtcc
     3361 gcccacgacc ccaccgaaca ggcgcgactg ggggccgcct acacccgcta taaggacatg
     3421 ctcgccgcat tgctgtaggg aagtgctaaa ggcgcacggc cttacgtgcc actatcagct
     3481 tgttagcgct tgctgatgct tcgcgcaagg ccctttgcct cacccagcag gctctggata
     3541 tcatcaaggg cgggaactcc gcgcgcggcc gcactcgatt ctgcgctaag tgatgcgctc
     3601 gcacgcgctt gagcctcttc gagtctagct ctcacctggt cgatgacttc ttccgcctta
     3661 gccccgttct cccgcgcctg gttgacttca cccactgcca gggccgcagt gatgcggtct
     3721 tgcagcgagc gcaagatgga agtgcctgcc gagccggcaa tcagggctgt ggccacaagc
     3781 ccgaagccgg tcctggggct aagcaccagg agtgcggcta cagcggccac ggctcccacg
     3841 atgatgcggg acacaatgcc caggtcaaac acatgccggc taacagcatg aggcagtttt
     3901 tcggggaact cctcatccg
//
LOCUS       DASRWZ010000691         6120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_605104, whole genome shotgun sequence.
ACCESSION   DASRWZ010000691 DASRWZ010000000
VERSION     DASRWZ010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605104"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFH60_10920"
     CDS             <1..733
                     /locus_tag="VFH60_10920"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET6314342.1"
                     /translation="CRVHVKLADHYMQVVGWMGAAATPAEPPGGHKSRLLSRVAATPQ
                     AAPATSAATEPIPFAPRTTAGPAMTAYSGKDAQGVPSSVADLAEYRARRAGRSRVLWS
                     GAAVAVLALLVLAGWLSSVLGRPYLPEGYKAIAVQNQPGGSASSAVLFYNPERRDAYF
                     QADGLQVLDAQKVYEMWLLPKGGGDPVPAGIFNAGGGGKVWHEIQAPADMAQYAGVAV
                     SLENAPGGKVVAGPILLMGQYSTTD"
     gene            complement(957..1910)
                     /locus_tag="VFH60_10925"
     CDS             complement(957..1910)
                     /locus_tag="VFH60_10925"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HET6314343.1"
                     /translation="MTRLKTYASLGAALVALGIGGLFTGSASAQTPGGAQRPNPHPVR
                     LQGEVKAVGTNSITLNTRGGEVTANVGPNTWIVVQKPDGPGQGSISDLKPNMPATVAG
                     MTTNDPRVVDARTITQGVGGDRRGAGRHRGPGPANVGGGTIKSISGNTLTVTNHRGRD
                     VQVETTADTVVLDSGFKTVSALKAGDRVQVLGVPQRVNRDARPTPDNLKVNAWAVRVV
                     RPGVELVGGQVEAVSGNTVTLKRLRNADGLPVNLSATTEYRKLTISATERKATLGSAT
                     QADAKAGSHIVVEGTRSADGKTFNATSVIIVPGKGDTAPQV"
     gene            complement(2050..2574)
                     /locus_tag="VFH60_10930"
     CDS             complement(2050..2574)
                     /locus_tag="VFH60_10930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET6314344.1"
                     /translation="MPIEKHLFLRVMGSFASGVTIVTVTDQEGVARGFTASAVSSLSL
                     EPRMLLVCVSERSSTLDIIKQAGSFAVNILAADQQHVAQQFATPDGDRFAGLRWRQGP
                     ETGNPVIDGSLAYAECRLTDTCKGGDHVIVIGEIVAGDSHEAEPLLYFRGRYGRYEAV
                     VAPVVYPADIWEIW"
     gene            complement(2804..3538)
                     /locus_tag="VFH60_10935"
     CDS             complement(2804..3538)
                     /locus_tag="VFH60_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6314345.1"
                     /translation="MSNSQHDQQDAPHAEPLSRHVTFEGREAGGEAPRLEGVLHVPQD
                     GSLHPGVVLLHANPAAGGNMDMKVIAAIEAALAAAGIATLRYNSRGVGNSGGTVSRVA
                     GKRLVAPEGEAEVGDIGAALDFLGGQDYVDESRLALVGHSFGARISLAYVAANPDERV
                     QAITCIGLPVAWRDLSYLGQWPRPRLFVTGELDDFCPPEELAEFVEGLPEPKTLLTIK
                     QTGHFFEGRERDLAIGVRAFLQQVLR"
     gene            complement(3554..4141)
                     /locus_tag="VFH60_10940"
     CDS             complement(3554..4141)
                     /locus_tag="VFH60_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6314346.1"
                     /translation="MDNQQGQPADPQAEASPAQQLGHQLRKGLDRIGFEADKLLRSNR
                     IRSEATRLQDQSDEKIYALGTKVVEMAERGMALEPELQTLVSQIKRLESELARKKKEL
                     EAISSEAWAEPEPPPALRPAPAKPALQAPRQPRQSGGRTAKPEQPTANAASATPAAST
                     GAPGPQQEAERICPVCKAQVRPQAVFCANCGHRLG"
     gene            complement(4198..5367)
                     /gene="rodA"
                     /locus_tag="VFH60_10945"
     CDS             complement(4198..5367)
                     /gene="rodA"
                     /locus_tag="VFH60_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HET6314347.1"
                     /translation="MRAVSLSNIDLPLILCTLLSCALGIVMVFSATNAAGAATFGFNS
                     FVTRQMIYAGAGILLMIAVARFDYRMLESFTVAFYVIVIGLLALVAVLGMTEYGSTRW
                     INLGIFPIQPSELAKLSLILMLAKFLAAHENEMSRFKWFVGAGLLMAVPAGIVLEQPD
                     MGTGSMLIFIFLGMAAAARVPARTFVASAVLAIPAIYVFWNYVMHDYQRSRLLVFLNP
                     ESDIFGEGYNLIQTRITIGSGGLFGQGYLHGMQTQGDFLKVQYSDFIFSVVAEQFGFI
                     GGVALVALLFILVWRCLVVASRAPDAYGSFIAVGVATWICMQVFIHVGMNIGLMPVTG
                     IPLPFISYGGSSLMSILLGLGLVQSVALRSSTVIFGGEPLRAGWARSARTTLRPR"
     gene            5583..5783
                     /locus_tag="VFH60_10950"
     CDS             5583..5783
                     /locus_tag="VFH60_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314348.1"
                     /translation="MPASDLLPYIFTNTRLPLAVQFARWVQESPRFRAFAETYRDKIR
                     KKVRGITEAEGYRDLQADLATA"
     gene            5811..>6120
                     /locus_tag="VFH60_10955"
     CDS             5811..>6120
                     /locus_tag="VFH60_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314349.1"
                     /translation="MEYEKYGVGKQRGQDFSITYKTHTRFDVEVTRLRPGQAGEAGVA
                     PEPGKLANTLCAKLEQLLPSMINVLVLAADDAHFSGDDSSLDAGQALENWLLSATTLL
                     Q"
BASE COUNT         1052 a         1914 c         2006 g         1148 t
ORIGIN      
        1 gtgccgggtg cacgtgaagt tggccgacca ctatatgcag gtggtgggct ggatgggggc
       61 ggctgccact cccgccgagc cgccgggcgg gcacaagagc cgcctgttgt cgcgtgtcgc
      121 cgccaccccg caagccgcgc ccgcgacgag cgcggcaacc gagcctattc cgtttgcgcc
      181 gcgcacaacg gcaggtccgg cgatgaccgc ctattccggc aaggacgcgc agggtgtgcc
      241 gtcttccgtg gcggacctgg ccgagtatcg cgcccgccgg gcaggcaggt ccagggtgct
      301 atggagtggg gcggcggtgg cggtgctcgc gctgctggtg ctggcgggct ggctgtcgtc
      361 ggtcctgggc aggccctact tgccggaggg ctacaaggct atagccgtgc agaaccaacc
      421 gggagggtcg gcgtcctcgg ctgtcctctt ttacaaccct gagcggcgag acgcttattt
      481 ccaggctgac ggcctacaag tcctcgacgc ccagaaagtg tacgagatgt ggctgctgcc
      541 gaagggcggt ggcgaccccg taccggcagg catattcaac gcgggcggcg gaggcaaggt
      601 ctggcacgag atacaggccc ccgccgacat ggcccagtac gccggtgtgg ctgtgagcct
      661 ggagaacgcg ccgggtggca aggtggtcgc cgggccaatc ctcctcatgg gccagtacag
      721 caccaccgac tagatggcac gagttgaaga aggcactacc aggagtacga agacgcaaag
      781 aacgcaaagg acgcgcaaag attaagatgg actagagcgt aatgagagga gcagggtacg
      841 atatcgagtg ccctgctcct ctggctttaa gagatcatct ttagtcttgg cgtggtcctt
      901 ggcgtccctt cggctgcgct cctggcgttc ttggcgtcct ggcggttccg tctcttctaa
      961 acctgggggg cggtgtcgcc cttgcccggc actatgatga ccgaggtggc gttgaacgtc
     1021 ttgccgtcgg cgctcctggt gccttctacg actatgtggc tgcccgcttt ggcgtcggct
     1081 tgggtggcgc tgcccagtgt tgctttgcgt tcggtggcgg agatggtgag cttcctgtac
     1141 tcggtggtgg cgctcaggtt gaccggcagc ccatcggcgt tacgcagccg tttgagcgtg
     1201 accgtgttgc cgctaaccgc ctccacttgc ccgcccacaa gctcgacgcc gggccgcacc
     1261 acgcgcaccg cccacgcgtt gaccttcagg ttgtcggggg tgggccgggc gtcgcggttt
     1321 actcgctgtg gcacgcccag tacctgcacc cggtctccgg ccttcaacgc gcttaccgtc
     1381 ttgaagccgc tgtcgagcac caccgtgtcg gcagtcgtct caacctgcac gtcgcggcca
     1441 cggtggttgg tgacggtcag ggtgttgccg ctgatagact tgatggtgcc accacccacg
     1501 ttcgcggggc cggggccgcg atgtctgccc gcgcctcgcc tgtcgccgcc cacaccctgc
     1561 gtgatagtgc gggcgtcaac taccctgggg tcgttggtgg tcatgcctgc cacggtggcg
     1621 ggcatgttcg gcttcaggtc gcttatgctg ccctggcccg ggccgtccgg cttctggacg
     1681 acgatccagg tgttggggcc gacgttggcc gtcacttcgc caccgcgcgt gtttagcgtg
     1741 atgctgttgg tgccaaccgc tttcacctcg ccttgcagcc gaacggggtg cgggttgggc
     1801 ctctgcgcgc ctccgggggt ctgcgccgaa gcggagcctg tgaacaagcc accgataccc
     1861 agggcgacca gggccgcccc caaacttgcg tatgtcttca accttgtcat gtacttcgtc
     1921 ctcctacttg gaccgtgtgt actgccgcgc aatctcctgc cgcggccgtt gtcagtctta
     1981 ttgtaggtcg ttgtcgcccg cgtaacgtta gggctttgta aaatctctgt aaaacggggc
     2041 cgtgacgggc taccaaattt cccagatgtc ggcgggatat accaccgggg cgaccaccgc
     2101 ctcgtagcga ccgtagcggc cccggaagta gagcagcggc tcggcctcgt gcgagtcccc
     2161 ggccacaatc tcgccgatga cgatcacgtg gtcgccgccc ttgcacgtat cggtcagtcg
     2221 gcactcagcg taggccaggg agccgtcgat gacggggttg cctgtctccg gcccctgccg
     2281 ccagcggagg cccgcgaacc tatcgccgtc gggggtggcg aactgttgcg ccacgtgctg
     2341 ttggtcggcg gcgaggatgt tgacggcgaa gctccccgcc tgcttgataa tatcgagggt
     2401 gctcgaccgc tcgctgacgc acaccagcag catacgcggc tccagcgaca gggaagatac
     2461 ggcgctcgcg gtaaagcccc gcgcgactcc ctcctggtcc gtcaccgtga cgatggttac
     2521 cccgctcgcg aacgacccca tcacgcgcag gaacaagtgc ttttctatcg gcatggttgt
     2581 ttacctcgat ggcttgctgt agtttgttgt tgctatacgc acgtagagta acatacgaag
     2641 ggacggaacc gccaagacgc caagataaga ttggtgtccc acgcaaaagg accagggtac
     2701 agcatcacgt accctggtcc tatttgtact cttctaaccc tttattctct ttgcgcgtcc
     2761 tttgcgttct ttgcgtcttt gcgttttcgt ctcctagcaa gtcctagcgt agcacctgtt
     2821 ggaggaaggc ccgcacgccg atagccaggt cgcgctctcg gccctcgaag aagtggccgg
     2881 tctgtttgat ggtgagcagg gtcttgggtt cgggcaaccc ctctacgaat tcggcaagct
     2941 cttcgggcgg gcagaagtcg tccagctcgc cggttacgaa gaggcggggg cgaggccact
     3001 gccccaggta gctcaggtcg cgccaggcga cgggcaggcc gatgcacgtt atcgcctgca
     3061 cccgctcgtc ggggttggcg gccacgtagg caagggaaat gcgcgccccg aacgaatggc
     3121 ccaccagggc gagccgcgac tcgtccacat agtcctgccc accgaggaag tcgagcgccg
     3181 cccctatgtc gcccacctcg gcctctcctt caggggccac aagtcgcttg ccagcgacac
     3241 gggagacggt gccgccgctg ttccccacgc cccgcgagtt gtagcgcaac gtggcgatac
     3301 cggccgccgc gagggccgcc tcaatcgccg cgatgacctt catgtccata ttgcccccgg
     3361 cggcggggtt ggcgtggagg agcaccacgc cggggtggag ggagccatcc tgaggcacgt
     3421 gcagcacgcc ttcaagccga ggggcttcgc cgcctgcctc gcgaccctcg aaggtcacat
     3481 ggcgcgacag tggctcggcg tgtggagcgt cctgctggtc gtgctgggag ttgctcatgg
     3541 gaagggtgcg cctttagccc agccggtggc cgcaattggc gcaaaagacg gcctgaggcc
     3601 gcacctgggc cttgcatacc gggcaaatcc tctcggcttc ctgttggggt ccgggtgcgc
     3661 cggtagaagc ggcgggtgtg gcggatgctg cattagctgt tggctgctcg ggcttggccg
     3721 tgcgcccgcc gctttgcctc ggctgcctgg gggcttgcaa agcgggcttg gcgggggcag
     3781 ggcgcagcgc ggggggcggt tcaggctcgg cccatgcctc gctgctgatc gcctcaagct
     3841 ctttcttctt acgcgcaagc tccgactcga gcctcttgat ctggctcacc agcgtctgta
     3901 gctccggttc cagggccatg ccgcgctcgg ccatctcgac caccttggtg ccgagggcgt
     3961 atatcttctc gtccgactgg tcctggaggc gggtagcttc ggagcggata cggttggagc
     4021 gcagcagctt gtcggcctcg aagccgatgc ggtccagccc cttgcgcaac tggtgcccaa
     4081 gctgctgtgc ggggctggct tcggcctggg ggtcggcggg ttgtccttgt tggttgtcca
     4141 tgggtgtgct cgcttatgag ggttgatagt ggtgtcggag catatacgca gcagggctca
     4201 gcgaggtcgc aaagtggtcc gtgccgagcg cgcccagccc gcccgcaacg gctcgccgcc
     4261 gaatatgacg gtgctcgagc gcagcgccac gctttgcacg aggcccagcc ccagcaagat
     4321 gctcatgagg gaggagccgc catagctgat gaagggcagc gggatgccgg tcacgggcat
     4381 gaggccgatg ttcatgccga cgtggatgaa gacctgcatg catatccagg tagccacgcc
     4441 caccgctatg aagctgccgt aagcgtcggg agcgcgcgag gcgaccacca ggcaccgcca
     4501 caccaggatg aacagcagcg cgacgagagc cacgccgccg atgaagccga attgctcggc
     4561 caccaccgag aagatgaagt cgctgtactg caccttgagg aagtcgccct gcgtctgcat
     4621 gccgtggagg tagccctgcc cgaacaggcc gccggagcca atcgtgatgc gcgtctggat
     4681 gaggttgtag ccctcaccga agatgtcgct ctccgggttg aggaagacga ggaggcggct
     4741 gcgctggtag tcgtgcatca cgtagttcca gaatacgtag atggcgggta tcgcgagcac
     4801 ggctgaagcg acaaaggtgc gggcgggcac tcgcgcggcc gcagccatgc ccaggaagat
     4861 gaagatgagc atcgagccgg tgcccatatc gggctgttcg agcacgatgc ccgcgggcac
     4921 cgccatcagg agacccgcgc ccacgaacca cttgaaccgg ctcatctcgt tctcgtgggc
     4981 cgcaaggaac tttgccagca ttaggatcag gctcagcttg gcaagctcgc tcggctggat
     5041 cgggaagatg cccaggttga tccaccgcgt tgagccatat tcagtcatgc ccagcacggc
     5101 taccagcgcg agcaacccga tgacgatcac gtagaaggcc accgtgaagc tctcaagcat
     5161 gcggtagtcg aagcgggcga cggcgatcat gagcaggatg cccgcccccg cgtagatcat
     5221 ctggcgagtg acaaagctgt tgaacccgaa cgtcgcggcc cctgccgcat tcgtggcgct
     5281 gaacaccatg acgatgccca gggcgcacga cagtaaggtg cacagtatga gcggtaaatc
     5341 gatattgctt aaactaacgg cacgcacgtt gcgattaccc tttgcaggct gatgtgataa
     5401 gtccctagat gataacacat gcccgccgcg aatcaaaagg cgtagcggca aactgctcta
     5461 cgaacaacag cgttccgcct cccgaccgat ccatcttgcg gcaaagtgcg ctacattagg
     5521 aacataggaa cggtctgcca aggaacgcca acctttatgc cggattgact gcccaactgc
     5581 ccatgcctgc cagcgatttg ctcccctata tcttcaccaa tacgcgccta ccgctcgcgg
     5641 tgcagttcgc ccgctgggtc caggagtcgc cccgcttccg cgcgtttgcc gaaacctacc
     5701 gcgacaaaat tcgcaagaaa gtgcgcggca tcaccgaggc cgagggttac cgcgatctgc
     5761 aagccgacct ggcgacggcc tagttcctca cactggaacg gcgggttctg gtggagtatg
     5821 agaagtacgg ggtgggcaaa cagcgagggc aggacttctc catcacctac aagactcata
     5881 cccgcttcga tgtcgaagtc acgcggcttc gtccgggcca ggcaggggag gcgggggtag
     5941 cgccggagcc gggcaagctc gccaacaccc tttgcgccaa gctggagcag ctcctgccga
     6001 gtatgatcaa cgtcctggtc ctggccgcag acgatgcaca ctttagcggg gacgattctt
     6061 cactggatgc ggggcaggcc ctggagaact ggcttctgag tgcaacgacg ctgctgcaag
//
LOCUS       DASRWZ010000692         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_193633, whole genome shotgun sequence.
ACCESSION   DASRWZ010000692 DASRWZ010000000
VERSION     DASRWZ010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193633"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1627
                     /locus_tag="VFH60_10960"
     CDS             194..1627
                     /locus_tag="VFH60_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6314350.1"
                     /translation="MLLSARRPRPFMLSFLVFAFVFTASAQAGTAHMQVEDETAALMR
                     FGSAPSVPDAGLQAIVDGVVGELPGTWGVAIKKLDTGQYASFNADERQVSASLYKLWV
                     LGELFRQVAEGTVSLDSRETVTAEDAAYDVLTGELRLPAGSDVQIARAAYLMVTLSDN
                     TAASLLVRVLGPGNISRFMSRNGLSDSVFDWEADEGPFTTPNDVLKLLEMLATSQMVD
                     AQSSSEMVDLMLGQQINNLLPSGLPDDTPMAHKTGALEDLLHDAGIVYGASGPYILVA
                     MSSELPDYGIAWDAMPALSEQVDAYFNARPTAPVRYFHETRQAVGHHFLKFWQEYGGA
                     ETFGLPIAPESLADGRVTQQFERARFEWDPAGAGGAQANVTLGSLGEERAAKLGLSWP
                     RGEDTGEGRYFEETGQSLAGEFYTYWLNHGGERVFGLPISPAAEMQSPADDKMYMTQW
                     FQRARMELRGDGSVVLALLGREMAPSR"
BASE COUNT          298 a          544 c          562 g          298 t
ORIGIN      
        1 gggtcggagc ggggcagtga aggcttgcga tagggttatc ttcagcataa gtagggccaa
       61 cgtgtgaaga ggggaagacc gcaggtaatg acctcgctcc cgcagcgcgc cattgcgact
      121 tgctttgcgc cgccgccccc ctgcgctata atgttgggac cgaaacctac gctacccctg
      181 gagtcgctct gtgatgctat tgtccgcgcg ccgcccgcgc cccttcatgc tatccttcct
      241 ggtcttcgcg ttcgttttca ccgcctcagc acaggccggt accgcgcaca tgcaggtcga
      301 agacgagaca gccgccctca tgcgcttcgg tagcgcgccc tcggtccccg atgccgggct
      361 gcaagctatt gtggacggag ttgtgggcga gttgccgggc acctggggcg tggcgatcaa
      421 gaagctggac acggggcagt acgcctcgtt caacgctgac gagcggcagg tctcggcctc
      481 cctctacaag ctgtgggtgc tgggcgagct tttccggcag gtggccgagg gcacggtgag
      541 cctcgacagc cgcgagacgg tcaccgccga ggacgccgcg tacgacgtgc tgaccggcga
      601 gttgcgcctg cccgccggta gcgacgtgca aatcgcccgc gccgcctacc tgatggtgac
      661 gctcagcgac aacaccgcgg ccagcctcct ggtgcgggtg ctcggacccg gcaacatcag
      721 ccgcttcatg agccgtaacg gcctctccga ctccgtcttc gactgggagg ccgacgaagg
      781 gccgttcacc acgcccaacg acgtgctcaa gctcctggag atgctggcga cctctcagat
      841 ggtagacgct cagtcgagca gtgagatggt tgacctgatg ctcggccagc aaatcaacaa
      901 cctccttccc tccggcctgc cggacgatac gccgatggcg cataagacgg gggcgctcga
      961 ggacctgcta cacgacgcag gcatcgtcta cggcgcgtca ggcccataca tcctcgtcgc
     1021 gatgtcgagc gagctgcctg actacggcat cgcgtgggac gccatgcccg ccctctcgga
     1081 gcaggttgac gcctacttca acgcccgccc caccgcccct gtccgctact tccacgagac
     1141 gcgccaggcg gtcggccacc acttcctcaa gttctggcag gaatacggag gcgcggaaac
     1201 gttcgggctg ccaatcgccc ctgaaagcct cgcggacggg cgggtcacgc agcagttcga
     1261 gcgcgcccgc ttcgagtggg accccgcagg cgccggtggt gcgcaagcta atgtgacact
     1321 gggcagcctg ggtgaagagc gggccgcgaa gcttggcctc tcatggccgc gaggcgagga
     1381 caccggcgag gggcgctact tcgaggagac cggacagagc ctcgcgggcg agttctacac
     1441 ctattggctc aaccacggtg gggagcgcgt gttcggtctg cccatctccc cagccgccga
     1501 gatgcaaagc cccgccgacg ataagatgta catgacacag tggttccaga gggcgaggat
     1561 ggagttgcgg ggggatggtt cggtggtgct tgcgttgctt gggagggaga tggcgccttc
     1621 gcggtgaggg taagagtgtc gatgacactt gtaacgtgct cgctatgagt cagctttagc
     1681 cctgccaata aattggcggg ct
//
LOCUS       DASRWZ010000693         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_125398, whole genome shotgun sequence.
ACCESSION   DASRWZ010000693 DASRWZ010000000
VERSION     DASRWZ010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125398"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..2239
                     /locus_tag="VFH60_10965"
     CDS             53..2239
                     /locus_tag="VFH60_10965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6314351.1"
                     /translation="MDPDLSIVILSWNVRDLLAGCLRSLPRAAAQWWDRSEVLVVDNA
                     STDGSAGMVRRDFLAVRLLLLPRNLGFSGGNNAGIMSARGKYVLLLNPDTVALPGAIA
                     SMAAYMEAHEEAGIVGPRLLNPDGTLQPSRRRFPSLATALVESTPLQRWLGNLPAIRR
                     FYMLDVPEGETQQVDWLSGAALLCRRETLSQAGLFDPGYFMFSEEVDLCKRVKDAGWE
                     IVYIPDAEIVHYGGQSTGQAVAARHIDFNTSKARYFRLHEGRAIGRLVRLYLLATYAV
                     QTASEAAKWLLGHKRELRAERLRMYARVLGSGLRERRPRRRHASVLLVTGEYPPAQGG
                     VGDYTCKLGQALEAAGLRHQVLTRPTEAASPGDLAGASVQPNQPGPFLSRRIDLRAVT
                     RSLRATGAPIAHIQYQTGAFEMRPTVNFLPWLLRLTRRGATVVTFHDVRVPYLFPKAG
                     PVREWANRLMARGAGACIATNPEDAARIKSWGVKRLELVPIGSNIPNNPPSGFNRQAW
                     RERWGAVEGTTLLAYFGFLNSSKGLDDLLRALPQLRERGDYRLLMVGGGLGSSDPTNR
                     ATASQLGELARQLGVEGSLIWTGYLEPREVSAALLCADMAVLPYADGASFRRGSLLAV
                     LEHGLPLVTTEPDLEIRISDPSLRSMASFEFGGSWPSLVDGENALLVRVGDVEGIVAA
                     VARVAGDGELRERLGRGAEELAGFFSWAAIAESHARLYRRLLGREM"
     gene            2444..>2745
                     /locus_tag="VFH60_10970"
     CDS             2444..>2745
                     /locus_tag="VFH60_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314352.1"
                     /translation="MTSLSLGSNVAASSERRKNPWVLPPAGIALVYFALAALWILLSD
                     QAAAAIFPEHNTFVWVQTYKGWFFGAFTAILLYFLIKRAEGAQEESELRFRTLVEQ"
BASE COUNT          467 a          870 c          922 g          486 t
ORIGIN      
        1 aggcaaagcg ggtgtgttat cgtgacggct gaacgtacaa cctgggggga gcatggaccc
       61 ggaccttagc atcgtcatac ttagctggaa cgtgcgcgac ctgctggcgg gctgcctgcg
      121 ctccctgccc cgggccgccg ctcagtggtg ggaccgcagc gaggtgctgg tggtggacaa
      181 cgcctccacc gacggcagcg ccgggatggt gcgccgcgac ttcctagccg tgcggctcct
      241 gcttctcccc cgcaacctcg gcttctccgg cggcaacaac gcgggcatca tgtcggcgcg
      301 aggcaagtac gtgctgctgc tcaaccccga tacggtggcc ctgcccggcg ccatcgcgag
      361 catggcggcg tacatggagg cgcacgagga agcgggcatc gtggggccgc gcctgctcaa
      421 ccccgacggt acgctgcaac cttccaggcg gcgcttcccc agcctagcca ccgcgctcgt
      481 ggagtccacc cctttgcaga ggtggctagg taacctgcct gctatccggc gcttctacat
      541 gctggacgtg cccgaaggcg agacccaaca ggtagactgg ctgagcgggg cggccctcct
      601 gtgcaggcgc gagacgctgt cgcaggcagg gctgttcgac cccggctact tcatgttcag
      661 tgaggaagta gacctctgca agcgggtgaa agacgcgggt tgggagatcg tctacatccc
      721 cgacgcggag atcgtgcact acggcggcca gagcaccggc caggccgtcg ccgcccgcca
      781 catcgacttc aacaccagca aggcccgcta cttccgcctg cacgaaggcc gcgccatagg
      841 caggctggtg cgcctctacc tgctagccac ttacgccgtg cagaccgcct ccgaggccgc
      901 caagtggctg ttggggcaca agcgggagct tcgcgccgag cggctgcgta tgtacgcccg
      961 cgtgctcggc agcggtctgc gggagcggcg gcccaggcgg aggcacgcca gcgtgctgct
     1021 cgttaccggc gagtacccac cggcgcaggg cggcgtgggc gactacacct gcaagctcgg
     1081 ccaggcgctc gaagccgccg ggttgaggca ccaggtgctc acgcgaccca ccgaagctgc
     1141 atccccaggc gacctcgcgg gtgcgtccgt ccagccaaac caacccggcc cgtttctctc
     1201 ccggcgcatt gacttgcggg cggttacaag gtcgttgagg gccacgggcg cgcccatagc
     1261 ccacatccag taccagaccg gggctttcga gatgcgcccc acggtgaact tcctgccgtg
     1321 gctgctgcgc ttgacgcggc gcggggctac cgtggtgacc ttccacgacg tgcgggtgcc
     1381 ctacctgttc ccgaaggcgg ggccggtgcg cgagtgggcc aaccgcctca tggcgcgggg
     1441 tgccggcgcc tgcatcgcca ccaacccgga ggatgccgcg cgcataaaga gctggggcgt
     1501 gaaacggctg gagcttgtac cgataggctc taacatcccc aacaacccgc cgtccggctt
     1561 caaccggcag gcgtggcgcg agcggtgggg tgccgtcgag ggtaccacgc tcctggccta
     1621 cttcggcttc ctcaatagct ccaaagggct ggacgacctg ctcagggcgc taccacaact
     1681 ccgagagcgc ggagattacc ggctgctcat ggtggggggc ggccttggct caagcgaccc
     1741 caccaaccgg gctacggcct cgcaactggg cgagctagcc cggcagttag gcgtcgaggg
     1801 aagtctcatc tggaccggct acctcgaacc ccgagaggta tccgccgccc ttctctgcgc
     1861 cgacatggcc gtgctgccct acgccgacgg cgcttccttc cggcgtggca gcctgctggc
     1921 ggtgctcgaa cacgggcttc cactggtaac gacggaaccg gatttggaaa ttcggatttc
     1981 ggacccttcg cttcgctcga tggcaagctt cgaatttggg gggagttggc ccagtttggt
     2041 ggatggggag aacgccttgc tggtgagggt gggggatgtg gaggggattg tggcggcggt
     2101 cgccagggtg gcgggggatg gcgagttgcg ggagaggcta ggcaggggcg cggaggaact
     2161 ggcggggttc tttagctggg cggctatagc cgagagccac gcacggctct acaggcggct
     2221 gctgggccgg gaaatgtagg ctcagacaat tacaggcgat agtatataat tatttgtatc
     2281 gctgtcaatg gacacttgtg ctatagtgta tactggtgtt gccggattcg ctgcaagcct
     2341 ggatccatgc aggtgcatct acccgctggc acaaacgcac cctggtcacg tgaatctatg
     2401 taactcgtgt gcggaccgca cgagtgaaag tgtcaggtga gccatgactt ctctatccct
     2461 gggcagcaat gttgctgcaa gcagcgagcg tcgcaagaac ccgtgggtcc tcccaccggc
     2521 gggtatagcg ctcgtctact tcgccctggc agcgctatgg atacttctat ccgaccaggc
     2581 ggccgctgcc atcttccccg aacacaatac cttcgtatgg gtgcagacct acaagggctg
     2641 gttcttcggg gcgtttacgg cgatacttct ttattttctg atcaagcgcg ccgagggcgc
     2701 gcaggaggag tccgagttgc gattccgcac gcttgtagag caaat
//
LOCUS       DASRWZ010000694         1872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_467950, whole genome shotgun sequence.
ACCESSION   DASRWZ010000694 DASRWZ010000000
VERSION     DASRWZ010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467950"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VFH60_10975"
     CDS             complement(<1..1224)
                     /locus_tag="VFH60_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314353.1"
                     /translation="MCSRCGLRFAPVSAAVGGAAGAAAVVAADELSAGPAQEFPGEDA
                     AYTPVEDEGTQTEPLAPGLVDADAGGDTGYDTGDDTGDEAVAAGEAYDPDEVDDAAFI
                     TGESVPRSAGETESGALAGRSARAGDDPIFASLPDASYDVPTAAAATGAVPDTSFNDL
                     DSEAYGTYGAEDTFDAPQAPEAPAAFDSPEAYAGPETLEAQDAPVIPAVLDTADVPHD
                     AALEVDPGPAPGAVMDVASGIARDAEDEPEIVGAPVAMSIPTTPITTLAVEDQQRSRN
                     QLLAAGVAAVAVLVLCLAIVLAATGGRAGDGTPTVEAGTSPTVPIIAAANTATSTAAV
                     ATPAPPTATAVVEVSTETTGPIVAPTDTAQPAGTEQPTDTPLPLPTDTSVPAPTDTEV
                     PAPTDTLVPVPTDTLV"
BASE COUNT          355 a          555 c          604 g          358 t
ORIGIN      
        1 cactagcgtg tccgtgggta ccgggacaag agtgtctgta ggcgcgggga cctcagtatc
       61 ggtaggcgcg ggcacggacg tatcggtggg caggggcagc ggtgtatctg taggctgctc
      121 ggtgccagcc ggttgcgcgg tgtctgtagg tgccacgata gggccggtgg tctccgtact
      181 tacctcaaca acggcggtgg cggtcggggg cgcgggcgtt gcgacggctg cggtggaagt
      241 ggcggtattg gcggcggcaa tgataggcac ggtgggcgag gtaccagctt cgacggtggg
      301 cgtaccgtcg cctgctctgc ccccggtggc cgccagcacg atggcgagac acaggaccag
      361 taccgcgacg gccgccactc ccgccgccag caactggttt ctcgaccgct gctggtcttc
      421 cacggccaac gtggttatag gggtagtggg aatagacatg gcgacgggcg cgccgacaat
      481 ctcaggctcg tcttcagcgt cgcgggctat gccggaggca acgtccatca ccgctccagg
      541 agccgggcct ggatcgacct ccagggctgc atcgtggggc acgtccgctg tgtcgagaac
      601 ggcggggata acaggagcgt cctgggcctc gagagtctca ggaccggcgt atgcttcggg
      661 gctatcaaaa gccgcaggtg cttcgggggc ctggggggcg tcgaaagtgt cctccgcacc
      721 ataggtaccg taagcctcgc tatcgaggtc attaaaggaa gtatcgggga cagcgccggt
      781 agccgcggcc gcagtaggaa cgtcgtatga ggcatccggc agggaggcaa agataggatc
      841 gtcaccggca cgggcgcttc ttcccgccag ggcaccgctc tcagtctcgc ccgcgctccg
      901 tgggacgctt tcgcccgtta tgaaagctgc atcgtccact tcgtctgggt cgtacgcttc
      961 gccagcggct actgcctcat ccccggtgtc atcaccggtg tcgtacccgg tgtcgcctcc
     1021 tgcgtctgca tctacaagac caggcgcaag tggctcagtc tgcgtgccct cgtcctcaac
     1081 cggggtgtaa gcagcgtcct cgccggggaa ctcttgagcc ggtcccgccg acaattcgtc
     1141 cgctgccacg acggcagccg cgccagccgc gccacccact gcggcagata cgggggcgaa
     1201 acgcaggccg caacgagagc acaccgtctg cccacgttct atgggggtgt cacacctggg
     1261 acaggcctca agtcctggag ttgttattag attcatattg tattcgcggt acttgtcggc
     1321 attattggct acgccacgga cccggcgctc taaatggggc gaggcgcaga tgacgtatgc
     1381 gcctacgcct aagcctggtg ctaccactaa ctgatgctag cttacactat tataacattg
     1441 tggaatatga ggctatttgc ctatgcacga atggcacatt agtaatggcc gccatgcgta
     1501 atagaccctg cgcggcaccc gttgcaggtt tgcggtgcat actcaccctg caagtaccct
     1561 gatggcatct caagagccaa cccacgcctt tgtcattctg aacgcagtga agaatctcgt
     1621 cccccaacga ccaggccgca cctttcttac agcacacagc cacaattaag catcgcctct
     1681 aaagaaaggt gtaacatcgc ggtgtaggac gagattcttc actgcgttca gaatgacaaa
     1741 gggaggtttg gtacgccaca agcaacgctc aagaaggggt ggccgagcct tcgcggagct
     1801 taccctgagc gtagacgaaa ggctcaggct gaccggggag cgatgtggtc ctccatgttg
     1861 ggcgacaggg gt
//
LOCUS       DASRWZ010000695         6027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_125495, whole genome shotgun sequence.
ACCESSION   DASRWZ010000695 DASRWZ010000000
VERSION     DASRWZ010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125495"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1165
                     /locus_tag="VFH60_10980"
     CDS             254..1165
                     /locus_tag="VFH60_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET6314354.1"
                     /translation="MSRLLVQGNVNLETTLRIPTFPIPYQPAQFVPHAVDIGVSGVGY
                     NLAKALSVLGDEVVLAAIVGRDTAASTIREQLRQDGFDGRYVLDRVGKTALSVIAYDA
                     TGKRAAFADLKDLMTQVYPHDLFSQALAGVEAVLFTQAAYCRPLLATARDAGKLVAVD
                     LQLISDLDDPYKRPYLEAAEIVFMSGEALPEPPEMWASNLLQRYPVVRVVGIGLGAEG
                     ALLAVRDTGLTLLVPTVAARPVVNTSGAGDALFACFMHYYLSTRDAEESLRRAVVFAS
                     YKTGAAGSSQGLLDEARLEQLYRSLHA"
     gene            1305..2357
                     /locus_tag="VFH60_10985"
     CDS             1305..2357
                     /locus_tag="VFH60_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6314355.1"
                     /translation="MSSKPERSKTKKPSIQDVARLAGVSQTTVSFVVNDAQEANIPAE
                     TRERVWSAVKELNWRPNALARGLISRRSHTIGFVSDGYVSDEMVAAPLAGKTIQGVQD
                     AAWADDKMVLVFNTGRNRKIERDAIEMLLERQVDGIIYATLFHHGVTPPAAISQVPTV
                     LLDCYAEDRSLPSVVPDEVQGGRTATEVLLEKGHRRVGFINNVDPMPAQTGRLEGYKQ
                     ALAGYGVAFDPRLVQYGTTIHTSGGYRCAMELMQLPEPPTALFCFNDLTAMGAYDALR
                     HLGLAIPGDVAVVGFDNLELIAAQLSPPLSTMELPHYQMGQWAVRYLLEHADHTGDVE
                     PVQHKIACPFIPRSSA"
     gene            complement(2480..4045)
                     /locus_tag="VFH60_10990"
     CDS             complement(2480..4045)
                     /locus_tag="VFH60_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HET6314356.1"
                     /translation="MSSFPNLRRAIALVMLLGLTVLAVSCVSADPATAPSPTPAAPSA
                     TPSPSPTPSGPRTVFVHLFEWKWTDVAQECETFLGPKGYAAVQISPPQEHLMAPGHPW
                     WQRYQPVSYKLESRSGTRAEFADMVSRCKAAGVDIYADAVINHMAGMDSGTGTAGTAF
                     THYSYPGLYEFADFHHCGLTGRSDDIFSYRDRKQVQTCELVNLADLDTASGKVQDRIA
                     AYLNDMLGLGVSGFRIDAAKHIDTNELKAIIGKLDKTTGGSEAYIYQEVIEGPGEPIT
                     ATEYLQNGDVSEFKFSSRVGPNFRNGQLAGMKEFALTRAFLPSDKAIVFTDNHDNQRG
                     HGAGGNVVTFKDGPLYNLANVYMLAWPYGYPQVMSSYNFDSSDQGPPSNPDGSTRPVY
                     SNGVPDCARENWVCEHRRPEIAGMVGFRNYTAPRFEVTDWWTNGGKAIAFGRGDLGFV
                     VINREDTAVTETFKTSMPGGTYCDVTRGEVTADGRDCTGPTITVSAAGEFVVPVPPTS
                     AVAIHGGAKVLGP"
     gene            complement(4337..5113)
                     /locus_tag="VFH60_10995"
     CDS             complement(4337..5113)
                     /locus_tag="VFH60_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HET6314357.1"
                     /translation="MNLLFIGDLVGPEAVAYLVGRLPALRREYNVNLVVANAENAAIT
                     ATTPWEGFGMTVRIVDDLLRAGVDVITSGNHGWDGPEMQEVHKHPRVLRPYNSPESMP
                     GKGLVHLEVQGEPVTVVNLSSVRGLIDGALPLYETWRTLDLRGTVIVDFHGDAAWEKM
                     IFATAIDGEAAAVLGTHTHEPVHKLHILPLGTALVTDVGMTGPTGSPGGFPLLHFAAK
                     YREEDYLALPPFELATGPVELGAVVLRIEKGKTQEIARVW"
     gene            complement(5135..5608)
                     /locus_tag="VFH60_11000"
     CDS             complement(5135..5608)
                     /locus_tag="VFH60_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314358.1"
                     /translation="MGGTPKAPMPQPEGTPNSDPTGLDLLAGTWRTDIFMPIEPPIAV
                     QGRTTCEWLEEGLFLVVRGSVEHPEFPSLLAIIGRDETTDAYSMLYFDSRGVSRIYAM
                     SLSTEAWKLWREAPGFFQRFVGTFSDDRNTITGYWEKSSDGSDWELDFNLTYTRI"
     gene            complement(5647..>6027)
                     /locus_tag="VFH60_11005"
     CDS             complement(5647..>6027)
                     /locus_tag="VFH60_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314359.1"
                     /translation="WAMSALGFARLGQGDYTRAEELFRESLRLAKTIDKHKIPDSLEG
                     LAEVECVLAEGGKALARLQRAATLLGAAEAIRGAMKSKIIPVRAPSRGRLVAEVRSRL
                     GPETYEQAYRLGEAMSFEEVLSLA"
BASE COUNT         1078 a         1913 c         1853 g         1183 t
ORIGIN      
        1 caccgggagg ttaccctttt ctcttgagac ggcatcatgc gctgtaagaa aagggccaat
       61 tgcgtccaaa aggacgagat ttctcactac gttcgaaatg acaaaggcgg cagccagtgc
      121 ccatatacac actcttatct cccacataca cactcttatc ttaagatgtc atcaagtgag
      181 tcattcaacc cgctgtctgt gaccttgctg ctttgtgaac aggctcccat gtagagccgg
      241 tggaaaggcc gccatgtccc gcctactcgt ccagggcaac gtcaacctgg aaactacgct
      301 ccgcatcccc acgtttccca ttccctacca gccggcccaa tttgtgccgc atgccgtcga
      361 tatcggggtg tcgggggtag ggtacaacct ggccaaagcg ctgtccgtgc tgggggacga
      421 ggttgtgctg gccgcgatag tcgggcgcga tacggcagcc tccactatta gagagcagtt
      481 gcgccaagac ggcttcgacg gtcgctatgt ccttgaccgg gtggggaaga cggccctatc
      541 ggtgattgcc tacgatgcga ccggcaagcg cgcggccttc gcggacctga aagacctcat
      601 gacccaggtc tatccccacg acctcttcag ccaggccctt gcaggcgtgg aggcggtgct
      661 gttcacccag gcggcctact gtcgcccgtt gctggcaacc gcccgcgacg ccggtaagct
      721 ggtggccgtt gaccttcaac tgatttcaga cctggacgac ccttacaaac gcccctacct
      781 ggaagctgcc gagattgtgt tcatgagcgg cgaagcgctg cctgagccgc cggagatgtg
      841 ggcctcgaat ttgctgcaac gttacccggt ggtgcgagtg gtaggcatcg gactgggggc
      901 ggagggggcg ttgctggcgg tccgcgacac gggcctaacg ctgctggtgc ccacggtggc
      961 ggcccgcccg gtcgtgaaca ccagcggtgc aggggatgcc ctgttcgcct gcttcatgca
     1021 ctactacctg tccacacgcg acgcggagga gtcgctgcgc cgggccgtag tgttcgcgtc
     1081 gtacaagacc ggggccgccg ggtccagcca gggcctactt gacgaagctc gcctggagca
     1141 gctatacagg tccctgcacg cgtagaggca tcggaacgaa agaaggagca tggctatacc
     1201 cctgccacgc gacacaaccc ccttgacacc gttacctgcc atccatatgc ttgttgctcg
     1261 atccccaaat ccgaaattcg aaattccaat ttccaaatcc gcctatgtcc agcaaacctg
     1321 aacgttccaa aaccaagaag ccttccatac aggacgtcgc caggctggcc ggggtctcgc
     1381 agaccaccgt ttccttcgtg gtcaacgacg cgcaggaggc caacatcccg gcagagacca
     1441 gggagcgcgt ctggtcggca gtcaaggagt tgaactggcg tccgaatgcg ctggcgcgcg
     1501 ggcttatctc gcggcggtcc catacgatag gctttgtctc agatggctat gtctcggacg
     1561 agatggtagc ggcccccctc gcgggcaaga cgatacaggg agtgcaggac gcggcctggg
     1621 ccgacgacaa gatggtgctg gtgttcaaca cgggccgcaa ccgcaagatc gagcgcgacg
     1681 cgatagagat gttgttggag cggcaggtcg atggcatcat ctacgcgacg ctgtttcatc
     1741 atggcgtgac cccacccgcc gctatttcgc aggtgcccac cgtgctgctg gattgttacg
     1801 ccgaagaccg ctcgctcccc tccgtggtgc ccgacgaggt gcagggcggg cgcaccgcca
     1861 cagaggtgct gctggagaag gggcaccggc gggtcgggtt catcaacaac gtcgatccca
     1921 tgcccgcgca aacgggccgc ctggaagggt acaagcaggc gctcgcaggc tacggtgtgg
     1981 cattcgaccc ccggctcgtg cagtacggga cgaccatcca caccagcggc ggctatcgtt
     2041 gcgcgatgga gcttatgcag ctaccggagc ctcccaccgc cctcttctgc ttcaacgacc
     2101 tcaccgcgat gggggcctac gacgccttgc gccacctcgg gctggcaatc cccggcgacg
     2161 tggcggtggt aggcttcgac aacctggagc tgatagcggc gcagctctct ccgcccctct
     2221 ccacgatgga actgcctcac taccagatgg gacagtgggc cgtgcgttac ctgctcgaac
     2281 atgccgacca cacgggggac gtggagccgg tgcagcacaa aatcgcctgc ccgttcatcc
     2341 cccggtcctc ggcctgagct gccgccttat atcaactccc tttgaagaga ttgtaaaaag
     2401 agaaagatag gagtgggaac aacccccagc ccccgtcagg ggagtacccc tgcacccccc
     2461 ttctcacgcc aacctgctat cacgggccga ggaccttcgc cccgccatga atcgccaccg
     2521 cgctcgtcgg cggcactggg acaacgaatt cgcccgccgc gctgaccgtt atggtcggtc
     2581 ccgtgcagtc ccgcccatcc gccgtcacct ctcccctcgt aacgtcgcag taagtaccgc
     2641 ccggcatgct cgtcttgaag gtctcggtca cggcggtatc ctcgcggttg atgaccacga
     2701 agcccaggtc cccgcgcccg aaagcgatag ccttgccccc attggtccac cagtcggtga
     2761 cctcgaacct gggtgcggtg tagttgcgaa agccgaccat cccggcgatt tccggccgcc
     2821 tgtgctcgca cacccagttc tccctggcgc agtcgggcac gccgttggag tagacgggcc
     2881 tcgtgctgcc gtcggggttg gagggtggcc cctggtcgga gctgtcgaag ttgtagctag
     2941 acataacctg cgggtagcca tagggccagg ccagcatgta gacgttcgcc aggttgtaga
     3001 gagggccgtc cttgaaggtg acgacgttgc cgcccgcgcc gtgccctcgc tggttgtcgt
     3061 ggttgtcggt gaagacaatc gccttgtcgc ttgggaggaa agcgcgggtc agggcgaact
     3121 ccttcatgcc cgcgagttgg ccgttgcgga aattggggcc gacgcggctg ctgaacttga
     3181 actcggacac gtcgccgttt tgcaggtact ccgtggccgt aattggctcg cccggccctt
     3241 ctatcacttc ctggtagatg tatgcctcgc tgccgcccgt cgtcttgtcg agcttgccta
     3301 ttatcgcctt gagctcgttg gtgtcgatgt gcttggcggc gtcgatgcgg aagcccgaca
     3361 cacccaggcc cagcatgtcg ttcaaatagg cggcgatgcg gtcctgcacc ttaccagacg
     3421 cggtatcgag gtcggccagg ttgaccagct cgcatgtttg gacctgtttg cggtcgcggt
     3481 agctgaagat gtcgtcgctg cgcccggtga ggccgcagtg gtggaagtcc gcgaactcgt
     3541 agaggcctgg gtagctgtag tgggtgaagg ccgtgcccgc tgtccccgtg ccgctgtcca
     3601 tgccggccat gtggtttatc accgcgtcgg cgtagatgtc cacccccgcc gccttgcagc
     3661 ggctgaccat gtccgcgaac tcggcgcggg tgccgctgcg gctttcaagc ttgtagctga
     3721 ccggctggta ccgctgccac caggggtggc cgggggccat caggtgctcc tgtggcgggg
     3781 aaatctgcac ggctgcgtac cccttggggc cgaggaaggt ctcgcactcc tgcgccacat
     3841 cggtccactt ccactcgaac aggtggacga acacagtgcg ggggccgctt ggcgtgggac
     3901 tggggcttgg cgtggcgctg ggggccgcag gggttggcga gggggcggtt gccgggtcgg
     3961 ctgatacgca acttaccgcc aggacggtca ggcccagcaa catcaccagg gcgattgccc
     4021 gccgcaggtt ggggaacgaa ctcatatgct actcccttgt cctcatcgcc atgaccgttg
     4081 tttcgtgtct gtgggcgagg atacttgcgc gtggtgcagc aagattaggg ccgcgcatga
     4141 actgacgagt aaccgggagt acagctactg aagctgctgc ggacgttcca actgacgtgg
     4201 ccggaccgct tcgacggagt ggcgatagag gccgtcgagt atgccgtgag caaggccgat
     4261 tgggagtaac acgaagccgc gcgtatttga ggcaggccat aactggcggt cggcggagcc
     4321 tatcgcgcgg cccggctcac catacgcggg cgatttcctg ggtcttgccc ttctctatgc
     4381 gcagcaccac cgcaccgagt tccaccggcc ctgtagccag ttcgaacggt gggagcgcta
     4441 agtagtcttc ctcacggtac ttggctgcga agtgcaagag cgggaagccc cccggactgc
     4501 cggtaggtcc cgtcatcccc acgtcggtaa cgagcgccgt acccagcggc aggatgtgca
     4561 gcttgtggac cggctcgtgc gtgtgcgtgc caagtacggc cgctgcttcg ccgtcgatgg
     4621 cagtagcgaa gatcatcttc tcccaggcag catcgccatg aaagtccact atcacggtgc
     4681 cacgcaggtc gagcgtgcgc caggtctcgt acaggggcag cgcgccgtct atgaggccac
     4741 ggacgctact caagttcact accgtaacag gctcgccttg cacctccagg tgtaccaacc
     4801 ccttgcccgg catgctctcc ggcgagttgt agggcctcag cacgcggggg tgcttatgta
     4861 cttcttgcat ctcagggccg tcccagccgt gattgccgga ggtgatgacg tctacccctg
     4921 cacgcagcaa atcgtccact atgcgcaccg tcataccgaa gccttcccac ggagtggtgg
     4981 ctgtaatcgc cgcgttttcg gcgtttgcga cgaccaggtt cacgttgtac tcacggcgca
     5041 gtgcgggtaa gcgtcccacc aggtacgcga ccgcctccgg tcccaccagg tctcctatga
     5101 aaagcaaatt catgatgagt agaagcccct atacttagat ccttgtatac gtcaggttga
     5161 agtctagctc ccagtcggag ccgtcgctcg atttctccca gtagcccgtg atggtgttgc
     5221 ggtcgtcgct gaacgtgcct acgaaacgct ggaagaaacc cggcgcttct ctccagagct
     5281 tccatgcctc cgtgctcaag ctcatcgcat agatgcggga gacgccacgc gagtcgaaat
     5341 agagcatgga atatgcatcg gtcgtctcgt ctctgcctat gatcgccagg aggctaggaa
     5401 actccgggtg ttcgacactc ccgcgcacaa cgaggaaaag gccttcctcc agccattcgc
     5461 aagtcgtgcg cccctgtacg gcgatgggcg gctcgatggg catgaatata tcagtacgcc
     5521 aggtgcctgc cagcaggtcc aggcctgtgg ggtctgagtt cggggtgcct tcgggttggg
     5581 gcattggggc ctttggtgtg ccgcccatgt tgcctgctcc tttcaccgaa cctggaagcg
     5641 aagaattcat gcaagcgaga ggacttcctc gaagctcatc gcttccccta gcctgtacgc
     5701 ctgctcgtaa gtttctggcc ccagcctcga acggacctca gccacgaggc ggcccctgct
     5761 gggcgctctg accgggatta tcttggactt catagccccc cggatggcct ctgccgcccc
     5821 aagcagcgtt gccgcgcgct ggagcctggc cagagctttg cccccttccg cgagcacgca
     5881 ttcgacctcg gccagccctt ccaggctgtc aggtatcttg tgtttgtcta tcgtttttgc
     5941 caggcgcagg ctctcacgaa aaagctcttc cgcgcgggtg tagtcgcctt gccccagcct
     6001 cgcaaaacct aacgccgaca tagccca
//
LOCUS       DASRWZ010000696         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_400553, whole genome shotgun sequence.
ACCESSION   DASRWZ010000696 DASRWZ010000000
VERSION     DASRWZ010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400553"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..743)
                     /locus_tag="VFH60_11010"
     CDS             complement(54..743)
                     /locus_tag="VFH60_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314360.1"
                     /translation="MSDIKWTLLDSTQKQATDSVTTLAGPTGSEQIAKGSYLTPSATQ
                     LDGAGFRFMAYVRDDGWDAIVQILKRTPGGSTVANWQIDLPLLNLNTMQPDPNGIGSK
                     ADGVSLVLDGATGNLWVYITSHTKPSPAPNIVIAWWEGELAGVGTPVQTGGYVPGPEP
                     CGPQNLPYFRPGAPLTRGECAEIVVRATWPGRTYTGPQKFQDVAPGAPFFEESGILSE
                     EGVVGGFACTP"
     gene            1060..1536
                     /locus_tag="VFH60_11015"
     CDS             1060..1536
                     /locus_tag="VFH60_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET6314361.1"
                     /translation="MKAAVLTVSDRAAAGVYEDRSGPVAAEFLRAKLHVEEVVTAIVP
                     DDREEVERALLQWVEVGGVRLVLTSGGTGISPRDVTPQATRNVLDYEVPGLAELMRAA
                     SFLKTPMAALSRATAGVRGRTLIINLPGSPKAVEECLEAILPVLPHAMSQLEGYGH"
     gene            1656..2081
                     /locus_tag="VFH60_11020"
     CDS             1656..2081
                     /locus_tag="VFH60_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314362.1"
                     /translation="MSADSNHGGESDRPAPGGALHDNLEVDAENPNIVRAIPATQRAG
                     DETAGNTSPGTRAEDDGYGDIIEGSDSERTGTSWGLPGREPGQTQTSAGEVPEGEGDG
                     EAHPNRNTGDRVTRDELDNVGSWSTIDTDDSAGSPSGFS"
     gene            2171..>2738
                     /locus_tag="VFH60_11025"
     CDS             2171..>2738
                     /locus_tag="VFH60_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314363.1"
                     /translation="MPGNVLAPSQPKKAVRAIVWPIVRNCALWALLHSLLASLPAKRA
                     AERVVGTRTRNGLYRVFYNAQAWLSFGWLVLSIARVPDRELLRLKGSAAKLVLLGRLG
                     AAGLTAWSGVILGPLRFNGVPQLLTYLEGKQPSPEDEAQGPILEHGRLKVAGPFRVIR
                     HPANAGPFLFALFSPRRTVKGLTLTVLLG"
BASE COUNT          527 a          833 c          880 g          498 t
ORIGIN      
        1 cggcgtaaac gcatgggcgc gcagtgggat tggcccgccg gcttaccgac ctgctatgga
       61 gtacacgcaa agccaccgac tacgccttct tcagacaaga ttccgctctc ctcgaagaag
      121 ggcgcgccgg gcgctacgtc ctggaacttc tgtggacctg tgtacgttct gccgggccac
      181 gtcgcgcgca ccacgatctc cgcgcactcg ccacgggtga gaggggcacc gggccggaag
      241 tagggtaagt tctgggggcc gcaaggttcg ggaccgggca cgtatccgcc cgtctggacc
      301 ggggtaccca cgcccgctag ctcgccctcc caccaggcta ttacgatatt gggtgcgggc
      361 gagggctttg tatggctggt aatatatacc cacaggttgc ccgtagcgcc gtccagcacg
      421 agtgacactc catctgcctt gctcccgatg ccgttggggt ccggctgcat cgtattcagg
      481 ttgaggagag gcaggtctat ctgccagttg gcgacggtac tccctcccgg cgtgcgcttc
      541 agtatctgca cgattgcgtc ccagccgtcg tcacgcacgt aggccatgaa tcgaaagccc
      601 gccccgtcga gctgagtagc tgaaggtgtc aagtagctgc ccttggctat ttgctcggaa
      661 ccggtcggcc ccgcgagcgt ggttaccgag tccgtcgctt gtttctgcgt agagtccaac
      721 agcgtccact ttatgtctga catgggtgtt ctcctttact cgataggttt ccggcctctt
      781 caccctggtt gaaaatgccg ggtgctctaa ccctcacggc ggaagtagcg acaccatcag
      841 cagccgcaga ccccacctta ggcgatgcgt atatcatagc ggtttttttc cgttccgcat
      901 cagccaatct gcgaggtagt cacatgctaa tacgcgcccc tggtagattg ttgcaggctt
      961 cggcggcgtt aggtgtaagc taccgggtgt ggcataatag ggcacgctac gggcacgaag
     1021 gccgaggtgg tgcccaagag aagcgtcagg agactgatta tgaaggcagc agtgctcacc
     1081 gtgagcgacc gggcggcagc gggggtgtac gaggaccgta gcggtcccgt agctgctgaa
     1141 tttctgcgag caaagttgca cgtagaagaa gtcgtcaccg cgattgtgcc ggacgaccgg
     1201 gaggaggtag agcgggcgct gctccagtgg gtagaggtgg gtggcgtgcg gttggtgctt
     1261 accagcggcg gcaccggtat ctcaccacgg gacgtgacgc cccaggccac gcgcaacgtc
     1321 ctcgactacg aagtgcccgg cctggccgaa ctgatgcgcg cggcctcgtt cttaaagaca
     1381 ccgatggccg cactctcccg cgccactgcc ggggtgagag gccgcacgct gattatcaac
     1441 ctgcccggct cacctaaggc ggtcgaagag tgcctggaag cgattttgcc tgtgctcccg
     1501 cacgcgatga gccaacttga agggtacggt cattagccaa agagtcaatt tcgtcagtag
     1561 caaaatagaa tatgtcagcc atgaaagcac atggaatggg ccccatcgtg gtgggcacac
     1621 taagtgctac tacctgaagg tataggaggt acgcaatgag tgctgattct aaccacggtg
     1681 gagagagtga ccggcccgcg ccgggcggcg cactccacga caacctggaa gtggacgcgg
     1741 agaaccccaa tatcgttcgc gccataccgg ctacgcaaag ggcgggcgat gagaccgcag
     1801 gcaatacatc gcccggcaca cgcgcggaag acgatggcta cggcgacatc atagaggggt
     1861 ccgacagcga gaggaccggc acgtcctggg gcctgccggg ccgcgagcct ggccagacgc
     1921 agacctcggc gggcgaagtg cccgagggcg aaggcgacgg tgaggcccac cctaaccgca
     1981 acaccggcga ccgcgtcacc cgcgacgagc ttgacaacgt cggcagttgg agcactatag
     2041 acaccgacga cagcgcaggc tctccgagcg gcttctctta gacctgttgc gcccacacgc
     2101 tacacgagcc cacttccggg ccgttgtacc tggaagtggg ctttggttcg tcactcgaaa
     2161 gggggcttaa atgccgggca acgtactcgc cccatcacaa ccgaagaagg cggtaagagc
     2221 aatagtgtgg cccatcgtgc gaaactgcgc gttgtgggcg cttctgcaca gcctgctggc
     2281 atcgctgccc gccaagcgcg ccgccgagcg ggtggtgggt accaggacgc gcaacgggct
     2341 gtaccgcgtc ttctacaacg cacaagcttg gttaagcttc ggctggttgg tgctgagcat
     2401 cgcgcgggtg ccggaccggg agctgctgcg cctcaaaggc tcggctgcga agcttgtcct
     2461 gctggggcgg ctaggcgcgg ccggactcac agcctggtcg ggcgtcatac tggggccgct
     2521 tcgcttcaat ggcgtgccgc agctgctcac gtacctggag ggaaagcaac catcgcctga
     2581 ggacgaggcg caaggcccca tcctcgagca cggcaggctc aaggtagccg ggccattccg
     2641 cgtaatccgg cacccggcga atgccggccc attcctgttc gccttgttct caccacggag
     2701 aaccgtcaag gggttgaccc tcaccgtgct gttgggcc
//
LOCUS       DASRWZ010000697         1684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1290923, whole genome shotgun sequence.
ACCESSION   DASRWZ010000697 DASRWZ010000000
VERSION     DASRWZ010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290923"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1352
                     /locus_tag="VFH60_11030"
     CDS             108..1352
                     /locus_tag="VFH60_11030"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6314364.1"
                     /translation="MIPAGERRLGLWRNRDYMLLWSGQVVSSLGSTVSAIVMPLFILV
                     LTGSPWLAGIAGALNTLPYLVFSLPVGALIDRWDRKLVMVLCDAGRAINMFSVPFAML
                     FDALTVWQLYAVTLVEGTLFVFFNIAEVAALPRVVRKDQLGDATSQNMATHAAANLVG
                     PAIGGYLFQVVGRGVPFLLDGISYMLSALSLLAIKTRFQGEKAAVERGTRRIGAEIKE
                     GLAWAWNEPLVRFTSFLSGGLNVVFSGGFLIIIVLAQNLGAGEAAIGTIFSIGSVGGI
                     VGAALGGRINRRFTLRQIVLATVWINVVCFPLYALAPSVLALGVIAAVVFISVPVYSV
                     AALSYRLALIPDALQGRVNSAVRLVAFGFQPVGSALAGFLLERVGAVPAVAVFSGLLL
                     VMGLLSLAMPLRPPDALPTSAS"
     gene            1388..>1684
                     /locus_tag="VFH60_11035"
     CDS             1388..>1684
                     /locus_tag="VFH60_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009050346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314365.1"
                     /translation="MEGSKVAREAGTIQIALVGDHDPNVKAHRAIPIALQMAAEAANC
                     RVAPDWIGTEELAADAAARLRAYDAIWVVPASPYRSMEGALAAIRYAREEGRPFL"
BASE COUNT          247 a          501 c          581 g          355 t
ORIGIN      
        1 tacgcgttac gtgttacgcg ttacgtgtta cgtgttacgt gttacgtgtt acgcgttacg
       61 tgttacgcgt tacgtgttaa tcatcgctac tctgctataa tgttcccatg attccggctg
      121 gcgagcggcg gttgggccta tggcgcaacc gcgactatat gttgctgtgg agcgggcagg
      181 tcgtgtcgtc gctgggcagc accgtctcgg ctatcgtcat gccgctgttc atccttgtgc
      241 tcaccggctc gccctggctg gcgggtatcg cgggggcgct caacacgctg ccttacctcg
      301 tcttcagcct gcccgtgggc gcgctcatcg acaggtggga ccgcaagctc gtgatggtac
      361 tttgcgacgc ggggcgcgcc atcaacatgt tcagtgtccc cttcgcgatg ctcttcgacg
      421 cgctcacggt gtggcagctt tacgcggtga cgctggtaga gggcacgttg ttcgtcttct
      481 tcaacatcgc ggaggtagcg gccctgcccc gcgtggtcag aaaggaccag ctaggcgatg
      541 cgacctcgca gaacatggcg acccacgcgg cggcgaacct cgtaggcccg gcgattggcg
      601 gctacctctt ccaggtggtg ggccgcgggg tacctttcct cctcgacggc atctcttaca
      661 tgctctcggc gctgtctctg ctggcgatca agacccgctt ccagggagag aaggcggccg
      721 tcgagagggg cacgcggcgg atcggggcgg agatcaagga gggcttagcc tgggcctgga
      781 acgagccgct ggtgcgcttc acttcgttcc tgagcggtgg gctgaacgtg gtcttttcgg
      841 gcgggtttct tataattatc gtgctggcac aaaacctggg ggcgggcgag gcggcgattg
      901 gcactatatt cagcataggg tccgtgggcg gcatcgtggg tgcggccctc ggcgggcgca
      961 tcaatcggcg ctttacgctg cggcagatag tgctggctac cgtgtggatt aacgtcgtgt
     1021 gctttccgct gtacgctctc gcaccgagcg tgctggcgtt gggggttatc gcggcggtgg
     1081 tctttatctc cgtgccggtg tacagcgtgg cggccctgag ctaccgcctg gcgctcatcc
     1141 ccgacgcttt gcagggccgg gtgaacagcg cggtgcggct ggtggcgttc ggcttccaac
     1201 cggtaggctc ggcgcttgcg ggcttcctgt tggagagggt gggggcggtg cccgcggtgg
     1261 cggtcttctc cggcttgctg ctggtcatgg ggctgctatc gctcgcgatg ccgctcagac
     1321 ccccggacgc tctgcccacg agcgcaagtt gagcctgtcg aacgaaacca ttccccagca
     1381 tctagatatg gagggtagca aagtggcgag ggaagcaggg accattcaaa tagccctggt
     1441 cggagaccac gaccccaacg tgaaggcgca ccgggcaatc cccatcgcct tgcaaatggc
     1501 tgctgaagcc gccaactgcc gggtagcgcc ggactggata ggcaccgagg agcttgctgc
     1561 ggatgccgcg gcccgcctgc gcgcttacga cgcgatctgg gtggtgcccg ccagccccta
     1621 ccggagcatg gagggcgcgc tcgcggccat acgctacgcg cgtgaggagg ggcggccctt
     1681 cctg
//
LOCUS       DASRWZ010000698         1905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1153671, whole genome shotgun sequence.
ACCESSION   DASRWZ010000698 DASRWZ010000000
VERSION     DASRWZ010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153671"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1451)
                     /locus_tag="VFH60_11040"
     CDS             complement(24..1451)
                     /locus_tag="VFH60_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6314366.1"
                     /translation="MPRYDAVVVGAGPNGLAAAITLARAGCSVLVIEAKETIGGGSRS
                     AELTLPGYVHDVCSAIHPLGVGSPFFRALPLEEHGLEWIHPQVPFAHPLDDGTAAVLH
                     RSVQATAETLGRDADAYRKLMLPLARDWETILDAILGPLRPLRILRHPFALARFGLGA
                     IQPARAFAERRFKGREARALFGGMAAHSMLALEQPPSAASGLMLGLLAHAVGWPVARG
                     GSQKIADAMSSYLRSLGGEIITGSPVASLDEVPQARAGLFDVTPRQLLRIAGDHLPEG
                     YKRQLGRYRYGPGVFKMDLALDGPIPWKARECLGAGTVHVGGTLREIAAAERAVTRGR
                     HPARPFVLVAQQSLFDPTRAPQGTHTVWAYCHVPNGSKLDMTERLEAQIERFAPGFRD
                     RIIGRSVMTPAQMEQYNPNYIGGDINGGVQDLLQLFTRPAPRLSPYTTPAREIYICSS
                     STPPGGGVHGMCGYFAAQDALKRIS"
     gene            complement(1459..>1905)
                     /locus_tag="VFH60_11045"
     CDS             complement(1459..>1905)
                     /locus_tag="VFH60_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6314367.1"
                     /translation="GYTAFSQPLLTRYSPVQLTGMSLVAAVPVLALAGVLAPPGLAYD
                     WSRFNVEAGLALLFGGILSVNVCYVIWYRGVQKLGGARTAVFSNMTPLVAIVVAWLWR
                     DEPLQPVYALGAALIAGGVLMTRCSRPTVHQKANVAAEADSAVGTG"
BASE COUNT          346 a          630 c          600 g          329 t
ORIGIN      
        1 cccctacaga tagatatcgg ccttcaagat attcgtttga gtgcatcctg cgcggcgaag
       61 taaccgcaca tgccatgcac gccaccgccg ggtggggtgg aggacgagca gatataaatc
      121 tccctggcag gcgtcgtata gggggatagc ctgggcgcgg ggcgcgtgaa tagctgcaac
      181 agatcttgta ccccgccgtt gatgtcgccg ccgatatagt tggggttgta ttgctccatc
      241 tgcgcgggcg tcatcacgct gcgcccaatg atgcggtcgc ggaagccggg cgcgaaccgc
      301 tcgatctgcg cctctagtct ctcggtcata tctagcttcg acccgttggg gacgtggcag
      361 tacgcccaga cagtgtgcgt gccctggggc gcgcgggttg ggtcgaagag gctctgctgc
      421 gccaccagta cgaacggcct tgcggggtgc ctgcccctgg tcacggcacg ctcggcggct
      481 gctatctccc ggagggtgcc ccccacatgg accgtgcccg cgccgagaca ttcccgcgcc
      541 ttccagggta tcgggccgtc cagcgcgagg tccatcttga acacgccggg gccgtatcta
      601 tatcgcccga gttgcctttt atagccttcc ggcaagtggt cgcccgctat gcgcaataac
      661 tgcctgggcg taacgtcgaa cagccctgcc cgcgcctgtg gcacttcatc caacgacgcg
      721 acggggctgc ccgtgattat ttctccccca agcgagcgca ggtaggaaga catggcatcc
      781 gctatcttct gtgagccgcc ccgcgccacc ggccacccaa cagcgtgagc cagcaggccc
      841 agcatcaagc cggatgccgc gctcggcggt tgctcaaggg ccagcatgga gtgggcagcc
      901 atgcccccga atagtgcgcg tgcctcccgt cccttgaatc gcctctccgc gaaagctcgg
      961 gccggctgaa tcgcgcccag gccgaaccgc gccagggcaa agggatgccg caatatacgt
     1021 agcggcctta gtgggccaag tatagcgtca aggatggttt cccagtcacg ggctaggggt
     1081 agcataagct tgcgataggc gtcggcgtcg cggcccaacg tctcggctgt ggcctgcacc
     1141 gacctgtgca gcactgccgc cgtgccatcg tccagggggt gcgcaaaggg gacctgcggg
     1201 tgaatccact caagcccgtg ctcttcaaga ggcaaggcgc gaaagaaagg tgaacctacg
     1261 ccgagaggat ggatagcaga gcaaacgtcg tggacgtagc cgggcagcgt aagctccgcc
     1321 gacctgctcc cgccgcctat cgtctcttta gcctcgatga cgagcacgga gcatccggcg
     1381 cgggcaagcg ttatggcggc tgccagcccg ttcggacccg ccccaaccac tacggcgtca
     1441 tacctgggca ctctctcgct accctgtgcc taccgcgctg tcggcctcgg ctgcgacgtt
     1501 tgctttctgg tgcacggtgg ggcggctgca gcgcgtcatc agcactcccc cggcgatcag
     1561 cgccgcaccc agtgcgtata ctggctgtag cggctcgtcg cgccagagcc acgcgaccac
     1621 tatcgcgacc aggggagtca tattggagaa gacggcggtg cgcgcccctc ccagcttctg
     1681 cacgccacga taccagatga cgtagcacac gttgaccgac aggatgcccc cgaagagtag
     1741 agccagccct gcctccacgt tgaaacgact ccaatcgtag gcaagtcccg gcggcgcgag
     1801 tacccccgcc agcgcgagca cgggcaccgc tgccaccagg ctcatgccgg tgagttgtac
     1861 aggcgagtaa cgcgtcaaaa gaggctggct gaacgcggta tagcc
//
LOCUS       DASRWZ010000699         8456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1016184, whole genome shotgun sequence.
ACCESSION   DASRWZ010000699 DASRWZ010000000
VERSION     DASRWZ010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016184"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..263
                     /locus_tag="VFH60_11050"
     tRNA            128..263
                     /locus_tag="VFH60_11050"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            311..430
                     /locus_tag="VFH60_11055"
     tRNA            311..430
                     /locus_tag="VFH60_11055"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            478..599
                     /locus_tag="VFH60_11060"
     tRNA            478..599
                     /locus_tag="VFH60_11060"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            632..765
                     /locus_tag="VFH60_11065"
                     /pseudo
     tRNA            join(632..665,728..765)
                     /locus_tag="VFH60_11065"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /pseudo
                     /anticodon=(pos:663..665,aa:Phe,seq:aaa)
     gene            1016..2710
                     /locus_tag="VFH60_11070"
     CDS             1016..2710
                     /locus_tag="VFH60_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HET6314368.1"
                     /translation="MKNLRAYTRKQDVTPQNEPIPGSTQVPNSAGGYAWAVDDWTRLD
                     RFMILGSEGGTYYITERKLTAENAQAVERCIAADGPRAVRRIVEISEAGRAPKNDPAV
                     SALALCATYGNDETKKAAFEALPRVCRTGTHLFHFVEFVDGLRGWGRGLRQAVAQWYA
                     MPPGKLAYQAIKYQNRDGWGHRDVLRLSHPKPPTEQHNAIYNWIAKGWEWVGEQPHPD
                     ETLRQLWAFERAKRAGSAGEIVQLITEYNLPREAVPTRWLTDIAVWEALLRRMPMEAM
                     LRNLATMTRIGLIAPTSEATRHVVEQLGNEERLRASRLHPIKVLSALTTYQAGKGVRG
                     KGTWQPVSQVVDALDGAFYKAFGNVPSTGKRWLLALDVSGSMGVPVMGIPGLSARSAS
                     AAMALVTAATEPQHTIVAFSSAGVGAYGGRWGGGEPGMSPVSISPRQRLDDVVKTLNK
                     IPMGGTDCSLPMRWALKRKVQCDVFVVYTDSETWYGDIHPVQALRQYRDKMGIPARLV
                     VVAMTSNGFSIADPNDAGMLDVVGFDTTTPAVMTDFVTGGAVSMQATADELAIAEA"
     gene            3120..4379
                     /locus_tag="VFH60_11075"
     CDS             3120..4379
                     /locus_tag="VFH60_11075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6314369.1"
                     /translation="MKQEPTHGSRVSRDRTVLAVSFAVFAAYVGTDMVVPVRVLYAQQ
                     QGASIAVIGAMATAVLLSNFIFQYPAGWLADRLGRRPVMIAGLVAQAAISLAYIFVPD
                     PVAFVGLRLIEGLASATMLPPARALIADAIPPEKRGEAYGVFNAFFNASLLLGPGIGS
                     GLAVFGYQGVFVAAIVARLLAVVVVVALIQGASRRKSGEGESVRRVSMRELFSLPLVG
                     AYILVFGDYLYLGFDLTIFPLWMQDKLGASILVIGLTYILWGVPTTLLSPVGGRWADR
                     VRRRSTLMLIFGAAQVPIYFVYGILETAWPVAALGLLHGAIYALMQPAIDAHVAASSA
                     EDVRARVQGVYSSIGLAGAFVGANGLTWLYEVDYRLPLFGLGATFGACVLVGGLIVRL
                     SESRRMLGGPGTGSAPLLEDTKDRGAT"
     gene            4376..5596
                     /locus_tag="VFH60_11080"
     CDS             4376..5596
                     /locus_tag="VFH60_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6314370.1"
                     /translation="MRFSIANCTVIDGLGGLPQEGVTVTVSGDTIEARARAQDAPPGE
                     FHPVYDGRGLTLLPGLIDMHVHMCLDPTTEERPDDPAEFAAIMTLNAAANLRKALFAG
                     ITTVRDVGGGLGVPMLVKRAWQEGRVVGARPFVAGSMITAPSGHGVEMGFGLEVEGPD
                     EVRRAVRHELSRGADLVKLVTFGVNTPSELGLDELRAGVEEAHRSGHRVACHAHFSRQ
                     SIENTLLAGCDTLEHGSLLDERLVDLMLARGTYYCPTIAVLEHIADTVKYYGGPESKF
                     RRVVRENMGNSRASVRLAHGGGVPIIAGTDAGTPGMEFDTLHDELRCLVDLGMSPHEA
                     IRCATGVAADGLGQPKMGRIAPGARADLLIVEGDPLSDLDVLRSPRAVFLDGKLLVGE
                     LPIYHPGQEAPDDA"
     gene            5589..5786
                     /locus_tag="VFH60_11085"
     CDS             5589..5786
                     /locus_tag="VFH60_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314371.1"
                     /translation="MLEFTPANEFERLLVQAGTDPGARPDFYRAFREADLIVLGYSGQ
                     SPGEGVTDRVRRPPASSLRSE"
     gene            complement(5885..8359)
                     /locus_tag="VFH60_11090"
     CDS             complement(5885..8359)
                     /locus_tag="VFH60_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET6314372.1"
                     /translation="MKHRRYRYHLARRGALCILGLLLLTLPLTGSISMSRAQQAVAVG
                     TLDEQATLVLRVYFRDVAERDRLAAELQPTEVGTEGGYLTLWASKATYDSLVARGLRV
                     EIAGQVEGIAARPDAAPLRQEGDTFYGGYRTVEGIYGFLDAQVESRPTLAEKVDFGDS
                     WCKAHAGACEFPEPTDGYDLWALHITNRDIPGPKPVFWFDAAIHPEEIATSEMAMRFI
                     SWLLDGYDTNPDARWLVDYHDIWVVPLVNPDGHHIAEAGGDQPYLQRKNADNDDGCSM
                     YPPGEAGAVGVDLNRNFPFKWACCGGASNSPCSHLYRGPHAASEEETQAVMGLIRRLV
                     PDQRGERDTDAAPLTTMGILQSMHAFASINIFPWGWSNSPAPNSTDLDNIGAHMSAPE
                     AGGNGYKHCASGAFDCLYRTDGNTKDWVYGELGAAAFTTELEGETFFPPYGELEGVWN
                     NNRGTLVYMAKIARAPYLLTRGPDANNVTVEPQVVDAGSRPRVSATINYNWTGNIYRQ
                     PVDAAEYYIDTPPWAGGTAHPMSPADGTLDGETEQVQALVDTTGLAAGRHIVFVRGRG
                     TSVYEGNPAWGPVSAAFLEVVPSGGTPVPSFPTAPPASTATTVPAASATAVAAASTPL
                     PPPVPLPGEARRTFPETGKTVSGTFLDYWQRNGGLAQQGYPISEVIGEVSDLNGQPYT
                     VQYFERAVFEYHPEHAGTPFEVLLSQLGTFRYRQKYPQGAGSASHSGQTPNTAAGSVL
                     FPETGKRLGGRFLDYWQRNGGLAQQGLPISDEFTEVSDLDGKPYTVQYFERAVFEYHP
                     EHAGTPFEVLLSQLGTFRYGEKYGQR"
BASE COUNT         1456 a         2647 c         2831 g         1522 t
ORIGIN      
        1 actccgacca gctgctcgca tgcgccaggt gggtagcggt atcgcgggta ctgcggtgat
       61 catgaaccat cgctatagat aagggggagg gtaatatcct cccaacttta caaccgaata
      121 cacacatggg gacgtagctc acataactca tcgccgcgtt cttgcccatc atgggccgtt
      181 ggtagatggt tagctggcat cgagcgcctt gcgcggagat cacccacgca aggaggttgc
      241 aggttcgagg cctgccgtcc ccaccgggcc gtcgcgtgga aacacgccgg cggcgcacaa
      301 ccgaatacat ggggacgtag cgtagtggac taacgcactt ttttccatca gccgctcttt
      361 gccttatggg ccgttggacg atggttaccg ctgtacggag cgatcgcggg ttcgaatccc
      421 gccgtcccca ccgagccgcc gcaacggaag acgcgggcgg cgaacaaccg aatacctggg
      481 gacgtagcgt agtggactaa cgcacttttt tccatcagcc gcttttcgcc ttatgggccg
      541 ttggaggatg gttaccactt tggatggaga gatcgcgggt tcgaatcccg ccgtccccac
      601 tgcgcacaac gcgctaaaca actaaataca tgggaacgta gctcagttgg tagagcagag
      661 gaaaaaaacg ccgtcaccgt acacttgcct cgctcgttgc ggggccgctg gtagatggtt
      721 atcttcagcc tccggtcgca ggttcgagtc ctgccgttcc caccatggcg gtacggtgtg
      781 cttgggttat ctttccgcaa caaaatggca ccttggaatg gtgggcttag gcccgaaccc
      841 agcccggagc ttgaccgcca gtagacaatc ttaagagcgg gccggagtaa tcaggttacc
      901 gagggctgtt tgagtctatg gctcggcagt catgggctgc tttgcagttg cctggctgca
      961 cgtttagccc gctctcaaca actttgcccc tatggggccg gaagggaggt gcgacatgaa
     1021 gaatctaagg gcatacactc gcaagcagga cgtgacgccg cagaacgagc cgataccagg
     1081 ctctacccag gtgcccaaca gcgcgggcgg ctacgcctgg gccgtggacg actggacgcg
     1141 cctcgaccgc ttcatgatcc tgggtagcga gggaggtacg tactacataa ccgagcgcaa
     1201 gctgacggcg gagaacgcgc aggcggtgga gcgttgcatc gcggcggacg gcccgcgggc
     1261 ggtgcgccgc atcgtggaga tatcggaggc gggccgcgcg cccaagaacg accctgcggt
     1321 gtccgcgctg gccctgtgcg ccacctacgg caatgatgag accaagaagg ccgctttcga
     1381 ggcgctgccg agggtctgcc gcaccggcac gcacctgttc cacttcgtgg agttcgtgga
     1441 cggcctgcgc ggctggggcc gtggcctgcg ccaggctgtt gcccagtggt acgccatgcc
     1501 gccgggtaag ttggcttacc aggccatcaa gtaccagaac cgcgacggct gggggcaccg
     1561 cgacgtgctg cgcctgtctc acccgaagcc gcccaccgag cagcacaacg ccatctacaa
     1621 ctggatcgcg aagggctggg agtgggttgg cgagcagccg caccccgacg agacgttgcg
     1681 ccagttgtgg gcgttcgagc gggccaagcg ggccggaagc gcgggcgaga tagtgcagct
     1741 catcaccgag tacaacctgc cccgcgaggc cgtgcctacc cgctggctca ccgatatagc
     1801 cgtgtgggag gcgctgctac ggcgcatgcc gatggaggcg atgctgcgca atctggcgac
     1861 gatgacccgt atcggcctga tcgccccgac gagcgaggct acccggcacg tggtggagca
     1921 gctaggcaac gaggagcggc tgcgggcttc gcgcctgcac cctatcaagg tgctgtcggc
     1981 cctcaccacc taccaggcag gcaagggcgt gcgcggcaag ggcacgtggc agccggtgtc
     2041 gcaggtagtg gacgcgttgg acggggcgtt ctacaaggcg ttcggcaacg tgccgagcac
     2101 cggcaagcgc tggctgctgg ccctcgacgt atcaggctcg atgggcgtgc cggtcatggg
     2161 cataccgggg ctgagcgcac gtagcgcgag cgcggctatg gccctggtga cggctgctac
     2221 cgagccgcag cacacgattg tggccttctc gtccgcgggg gtgggcgcgt acggcgggcg
     2281 gtggggcggg ggcgagccgg gcatgtcgcc cgtgtcgatc tcgccgcgcc agcgcctgga
     2341 cgacgtggtc aagacgctca acaagatacc gatgggcggc accgactgct cgctgccgat
     2401 gcgctgggcg ctgaagcgca aggtgcagtg cgacgtgttc gtggtctaca ccgactcgga
     2461 gacgtggtac ggcgacatcc acccggtgca ggcgttgagg cagtaccgcg acaagatggg
     2521 catcccggcc aggctggtcg tggtggcgat gacctccaac ggcttctcca tcgcggaccc
     2581 caacgacgcg ggcatgctcg acgtggtagg cttcgacacc actaccccgg cggtcatgac
     2641 cgacttcgtg accggcggcg cggtctcaat gcaggcaacc gctgacgaac tggcaatagc
     2701 ggaagcctag aagcaacagc ataccccgta gcgagtgggc actataacct gctcgctgtg
     2761 gggtatgccg ttgtagaagc tatcaaagcg acggtgtgga acggtgcaac caagcccctc
     2821 tgtgtcatcc tgagcgcagc gaaggatcag gtgggtaggg aagaagctgt attggagtag
     2881 ccacaacgcc ttgccaccag gctcaccact cccaccagcc tcacgcattg ccacagagac
     2941 cgactgccac ctgatccttc gctacgctca ggatgacaag gggaatatag tcctcttgtt
     3001 tgttacttac ttgcaagacc caccactgcg cgaaaatcgc ctgttactca caacccttgc
     3061 ctggtaacgc acgaactcgg ggccggtgcc tccgatttga tcttctgtta gagttaagca
     3121 tgaagcaaga acccacgcat ggaagcaggg tgtcgcggga ccggacggtg ctggccgtgt
     3181 cgttcgcggt gttcgccgcc tacgtgggca ccgacatggt ggtgccggtg cgcgtgcttt
     3241 acgcccagca gcagggggcg tccatcgcgg tcatcggggc gatggcgacc gccgtcctgc
     3301 tctccaactt catctttcag taccccgccg ggtggctggc cgaccgcctg gggcgcagac
     3361 ccgtcatgat cgcggggctg gtggcccagg ccgccatatc gctggcctac atcttcgtgc
     3421 ccgaccctgt cgcgttcgtc ggcttgcggc tcatcgaggg cctggcgagc gccaccatgc
     3481 tcccacccgc cagggcgttg atagccgacg ccataccgcc tgagaagcgg ggcgaggcgt
     3541 acggggtgtt caacgccttc ttcaacgcca gcctgctgct cggccccggc atcgggagcg
     3601 ggctggccgt ctttggatac cagggggtgt tcgtggccgc tatcgtggcc cgcttgctgg
     3661 cggtagtggt ggtagttgcg ctcatccagg gggcgtctcg ccgcaagtcg ggcgaaggcg
     3721 agagcgtgcg gcgcgtaagc atgcgggaat tgttctccct gccgctggtg ggggcttaca
     3781 tcctggtgtt tggcgactac ctgtacctcg gcttcgacct caccatcttc ccgctctgga
     3841 tgcaagacaa actgggcgcg tcgatacttg ttatcggcct gacctacatc ctctggggcg
     3901 tgcccaccac cctgctctca cccgtgggcg ggcgctgggc cgaccgggtg cgccgccgct
     3961 ccactctgat gctgatattt ggcgctgccc aggtgccgat ctatttcgtg tacggcatac
     4021 ttgagaccgc ctggcccgtg gcggcgcttg gcctgctgca cggcgctatc tatgccctca
     4081 tgcagcccgc tattgacgct cacgtcgcgg cctcatcagc cgaagatgtg cgggcgcggg
     4141 tgcagggtgt atactcctcg atagggctgg cgggggcgtt cgtgggggcc aacggcctca
     4201 cctggctgta cgaggtggac taccgcctgc cgctgttcgg gctgggcgcg accttcggcg
     4261 catgcgtgct ggtgggcggc ctcattgtgc gcttgagcga gtcccgccgc atgctgggag
     4321 gcccaggtac cggctccgcg cccctgctcg aagacacaaa ggaccgaggg gctacatgag
     4381 gttttcgatt gccaactgca ccgtaatcga cgggctgggc ggtctgccac aggagggcgt
     4441 caccgtcacc gtgagcgggg acaccataga ggctagggcc cgcgcgcaag atgctccgcc
     4501 gggcgagttt caccccgttt acgacgggcg cggcctgacg ctgcttcccg gccttatcga
     4561 catgcacgtg cacatgtgcc tggaccctac gacagaggag aggcccgacg acccggcgga
     4621 gttcgcggca atcatgacgt tgaacgccgc agccaacctg cgcaaagcgt tgttcgcggg
     4681 tatcaccact gtgcgtgacg ttggcggggg gctgggcgtg cctatgcttg tcaagagggc
     4741 gtggcaggag gggcgggtgg taggtgcgcg cccgttcgtg gcaggctcga tgatcaccgc
     4801 gccgagcggc cacggcgttg agatgggctt cgggcttgag gtggaaggcc ccgacgaggt
     4861 acgccgggcg gtgaggcacg agctatcgag gggagccgac ctggtcaagc tggtcacgtt
     4921 cggggtgaac acgcccagcg aactgggcct cgatgagttg cgggcgggcg tagaagaggc
     4981 ccaccgcagc ggccacaggg ttgcctgcca cgcgcatttc agcagacagt cgatagaaaa
     5041 caccctcctg gcaggctgcg ataccctgga gcatggcagc ctgctggacg agaggttggt
     5101 agacctcatg ctggcgcgcg gtacctacta ctgcccgacc attgccgtgc ttgagcatat
     5161 tgccgacaca gtgaagtatt acggcggccc cgaaagcaag ttccgccggg tggtaaggga
     5221 gaacatgggc aacagcaggg cgagcgtgag actggcccac ggcggtggag tgcctatcat
     5281 agcgggcacc gacgcgggca cgcccggcat ggagttcgac acgctgcacg acgagctacg
     5341 gtgcctggta gacctgggca tgtctccaca cgaggccatc aggtgcgcaa cgggcgtggc
     5401 ggcggacggc ctggggcagc ccaagatggg tcgaatcgcg ccgggcgcgc gtgccgacct
     5461 cctcattgtg gagggcgacc ccctgtcgga cctcgacgtg ctgcgcagcc ccagggccgt
     5521 gtttctcgac ggcaaactgc tcgtgggcga actgcccatt taccaccccg gccaggaggc
     5581 tcccgacgat gcttgaattt acccccgcca atgagtttga gaggctgctg gtgcaggccg
     5641 gcacagaccc cggcgcacgt cccgacttct accgcgcctt ccgcgaggcc gacctgatcg
     5701 tgctcggcta ctccggccag tcgccagggg aaggcgtcac agatcgagta cgacggccac
     5761 ctgcttcctc gctacgctcg gaatgacaaa gtcccccctt ggtcctgcgt tgggcactta
     5821 ccggacgcac cactcagaat gacaaagacc accttaccgc ctggtttgca gatactccct
     5881 gccctcatct ttggccgtac ttctccccat agcggaaggt gcccaactgg gagagtagca
     5941 cctcgaacgg agtgcccgcg tgctcggggt gatactcgaa caccgcgcgc tcgaagtact
     6001 gcaccgtgta cggcttacca tcaagatcgc tcacctccgt gaactcatcc gagatcggaa
     6061 ggccctgttg cgccaggccg ccgttgcgct gccagtagtc caggaaccgg ccgccgaggc
     6121 gcttgcccgt ttcggggaag aggacggaac cggcggcggt attgggcgtc tggcctgaat
     6181 gcgaagcaga acccgcgccc tgtgggtact tctgcctgta gcggaaagtg cctagctggg
     6241 agagcagcac ctcgaatgga gtgcccgcgt gctcggggtg gtactcaaac accgcgcgct
     6301 cgaagtactg cacagtgtag ggctgcccgt tcaggtcgga cacctcgcct atcacctccg
     6361 agattggata gccttgttgc gccagacctc cattgcgctg ccagtaatcg aggaaggtac
     6421 cgctaaccgt cttgcccgtt tcggggaagg tgcggcgggc ctcgccgggg agcggcacgg
     6481 gcggcggtag cggtgtggag gcagcagcga ccgccgtagc ggaggccgcc ggtacggttg
     6541 tcgcggtcga agcggggggc gctgtgggga agctcggcac gggcgtgccc ccggagggca
     6601 caacctcgag gaacgcggcg gagaccggcc cccaggcagg gttgccttcg tagacactgg
     6661 tgcctcgccc gcgcacgaag actatatgcc tgcctgccgc cagtccggtg gtatccacca
     6721 gggcttgcac ctgttcggtc tccccgtcga gcgtgccatc ggcaggagac atcgggtgcg
     6781 ccgtgccgcc cgcccagggg ggcgtgtcga tgtagtactc ggctgcatcc accggctggc
     6841 ggtagatgtt gcccgtccag ttgtagttga tggtcgcgct gacgcggggc ctagacccgg
     6901 cgtccactac ctgcggctcg acggtaacgt tgttggcgtc cggccctctc gtaagcaggt
     6961 agggcgcgcg ggcgatcttc gccatgtaga cgagcgtccc gcggttgttg ttccataccc
     7021 cctctagctc gccgtatggg gggaagaagg tctccccttc aagctcggtg gtgaaggccg
     7081 ccgcgcccaa ctcgccatac acccagtcct tggtgttacc atcggtgcgg tacaggcaat
     7141 cgaaggcccc ggacgcgcaa tgcttgtagc cgttgccgcc ggcctcaggg gcgctcatgt
     7201 gcgcgccgat gttgtccagg tcggtgctgt tgggagcggg cgagttcgac cagccccagg
     7261 gaaagatatt gatgctggcg aatgcgtgca tgctctgcag tatgcccatc gtcgtgagag
     7321 gggcggcgtc cgtatcacgc tcccctcgct ggtcgggcac cagcctgcgg atcagtccca
     7381 ttactgcctg cgtttcctcc tcggaggccg cgtgcgggcc gcgatacagg tggctgcacg
     7441 gggagttgga agcgcctccg cagcaggccc acttgaaggg gaagttgcgg ttcaggtcaa
     7501 cccccaccgc ccctgcctct cccggtgggt acatcgagca gccgtcgtcg ttgtcggcgt
     7561 tcttgcgctg taagtaaggc tggtcgcccc cggcctccgc gatgtggtgg ccatccgggt
     7621 tcacaagggg taccacccag atatcgtggt agtccaccag ccaccgggcg tccggattcg
     7681 tgtcgtagcc gtcgagcagc cagctaatga agcgcatcgc catctcggag gtggctatct
     7741 cctccgggtg gatggccgcg tcgaaccaga agaccggctt gggaccgggg atgtcgcggt
     7801 tggtgatatg gagcgcccac aggtcgtacc cgtcggttgg ttcggggaac tcgcacgcac
     7861 cggcgtgggc cttgcaccac gaatcgccaa aatcgacctt ctcggccagg gtagggcgac
     7921 tctccacctg cgcgtcgaga aagccataga tgccttccac ggtgcggtag ccgccgtaga
     7981 acgtatcacc ctcctgccgg agtggggccg cgtcggggcg ggcggctatt ccctctacct
     8041 ggcccgcgat ttccacccgc aggccgcgcg ccaccaggct gtcgtaggtc gccttgctgg
     8101 cccaaagcgt caggtagcca ccctcggtgc ctacttccgt gggttgcagc tcggcggcga
     8161 gacggtcccg ctcggccacg tcgcggaagt aaacgcgcag cacgagcgtg gcctgctcat
     8221 cgagcgtgcc cactgctaca gcctgctggg cgcggctcat actaatgctt cccgtgagag
     8281 gcagcgtgag caggagcaac cccagtatgc acaaagcgcc gcgcctggcg aggtggtaac
     8341 ggtaacgtct atgcttcaca gcccactgcc tcctgttcgg gttgagaggt cgctttggcg
     8401 tccgagcgcc aaggttctat gtccctttag ctattatcgc gcaacgaggg ccattg
//
LOCUS       DASRWZ010000700         3188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_537221, whole genome shotgun sequence.
ACCESSION   DASRWZ010000700 DASRWZ010000000
VERSION     DASRWZ010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537221"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..2953)
                     /locus_tag="VFH60_11095"
     CDS             complement(80..2953)
                     /locus_tag="VFH60_11095"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HET6314373.1"
                     /translation="MAKNPMQQLLEAGQSPWLDNIRRGLLTSGELQRMIDEDGIVGVT
                     ANPTIFEKAISGSTDYDTAIDDLVRQGKSSADIYKILIIEDISRAADILRPVYDRTDG
                     GDGYISIEVAPGLAHDTQGTINEAQEFWDAIKKPNIFIKIPATHEGIPAIEECLYRGI
                     NVNITLIFSVDVHRQVMEAYLSALERRVREGKPVDHVASVASFFVSRVDTKADKALED
                     LLKQTPDVDKQQQIKGLLGTTAINNSKLAYQAFLEVFKGDRWENIKRHGGRVQRPLWA
                     STSTKNPNYRDVIYVEELIGPDTVNTMPEATVRAFKDHGVVARTIDKDMDLANQQLQQ
                     LEELGISLQRITEELTVEGVESFSKSFVSLDECIGAKVDSLVSGLSERYGASLGGLQP
                     QVDDGLRLASKNQYVSKIWEKDAKFWKQDDADAQKKIKNRLGWLGVVHLMQDRAAELK
                     QFAQEIVDEGYTSVVLLGMGGSSLAPEVVNRVFGQAQGYPRFFMLDSTDPGTIEAIER
                     RIDPATTLFIVATKSGGTTETMSFYRYFRSRATAGKRDRAGENFVAITDPGSSLETLA
                     REEGFRRTFLNMPDIGGRYSALSYFGLVPAALMGLDIDALLSRAETMVEACAPCVNSE
                     DNPGLWLGTVWGTAAREGRDKLTILTSPDLDSFGLWAEQLIAESTGKEGKGLIPIAGE
                     PAGRPSAYGADRLFVYLRIDGATDAHQEHNIQKLEDAGHPVVRLNMRGKLDLGAEFFR
                     WEFATAVAGQMLGIDPFDEPNVQESKDNTQRLLDYYEQNGRLPQADALIEGEAFDLYA
                     DQEALARAGFEGTIETGIRALLSTAQEGVDYVAIMAYMPSTGEHEELFQDARTAVRDT
                     LRVATTFGYGPRFLHSTGQLHKGGPNRGIYIQVTAEPQRDVSIPGSPYSFGTLIRAQA
                     MGDKQSLEKQGRRVIRLHVKGDHAAGVEAIREVVREAIEPLGI"
BASE COUNT          502 a         1001 c          988 g          697 t
ORIGIN      
        1 actgaagcgg tgtcgatcag tcggaacttg aacagccata cacgcccctc gtttcttaac
       61 cccgatgctg tgaattcacc taaatcccta gcggctcgat tgcttctctt accacctccc
      121 gtatagcctc gacgcccgcc gcgtggtcgc ccttgacgtg caggcgaatg acccgacggc
      181 cctgcttctc cagcgactgc ttgtcgccca tcgcctgcgc ccgtatgagg gtgccgaagc
      241 tgtagggcga gcctggtatg ctcacgtcgc gttgcggctc ggcggtcacc tggatgtaga
      301 tgcccctgtt cgggccgccc ttgtgcaact gcccggtcga gtgcaggaag cgcgggccgt
      361 agccaaaggt ggtcgccacc cgtagcgtat cacgtacggc ggtgcgggcg tcctggaaga
      421 gttcctcgtg ctccccggtg ctcggcatgt aggccattat tgctacgtaa tcgacgcctt
      481 cctgggcagt gctcagcagg gcgcgaatgc cggtttcaat cgtgccctcg aagcccgcgc
      541 gggccaatgc ctcctggtcg gcgtacaggt cgaacgcttc gccttcgatg agggcatccg
      601 cctggggcag cctgccgttt tgctcgtagt agtcgagcag gcgctgggtg ttgtccttgc
      661 tctcctgcac gttcggctcg tcgaaggggt ctatccctag catctgcccg gcgaccgcgg
      721 tggcgaactc ccagcggaag aactcggccc ccaggtcgag cttgccgcgc atgttcaggc
      781 gcacgactgg gtgccccgcg tcctccagct tctgaatgtt gtgctcctgg tgagcgtcgg
      841 tcgcgccatc tatcctgagg tacacaaaca gcctgtcggc cccgtaagcg gaggggcggc
      901 ccgcaggctc tccagcgatt gggatgaggc ccttgccctc tttgccggtg ctttcggcta
      961 tcagttgctc ggcccacagc ccgaagctgt cgaggtcggg cgaggtgagg atggtaagct
     1021 tgtcgcggcc ctcgcgggcg gcggtgcccc acaccgtgcc aagccacagg ccggggttgt
     1081 cctcggagtt cacgcagggc gcgcaggcct ccaccatcgt ctcggcccgg ctgagcagcg
     1141 cgtctatgtc gaggcccatc agcgcggcgg gcacgaggcc gaagtaggag agggccgaat
     1201 agcgcccccc gatgtcgggc atgttgagga aggtgcggcg gaagccctct tcccgcgcca
     1261 gcgtctccaa cgacgagccg gggtcggtaa tcgccacgaa gttctcgccc gccctgtcac
     1321 gcttacccgc ggtggccctg ctacggaagt agcggtagaa gctcatcgtc tcggttgtgc
     1381 cgcccgactt ggtagccacg ataaagaggg tggttgccgg gtcgatgcgc cgctcgatgg
     1441 cttctatcgt gccggggtcg gtcgagtcga gcatgaagaa gcggggatag ccttgcgcct
     1501 gcccgaacac gcggttgacg acctcgggcg cgagggagga gccgcccatg ccgaggagca
     1561 ccacgctggt gtagccctcg tccacgatct cctgggcgaa ctgctttagc tcggcggcgc
     1621 ggtcctgcat caggtgcacc acgccgagcc agccgaggcg gttctttatc ttcttctggg
     1681 cgtccgcgtc gtcctgcttc cagaacttcg cgtccttctc ccagatcttg ctgacgtact
     1741 ggttctttga ggcaagccgc aggccgtcat cgacctgcgg ctgtagcccc cccaggctgg
     1801 caccgtacct ttcggacagc cccgacacta gcgaatccac tttcgcgccg atgcactcgt
     1861 ccaggctcac gaagctcttg ctgaacgact cgacgccctc cacggtaagt tcctcggtaa
     1921 tgcgttgcag ggagatgcca agctcctcaa gctgttgtag ctgttggttg gcgaggtcca
     1981 tgtccttgtc aatcgtgcgg gctaccacgc cgtggtcctt gaaggcgcgc acggtggcct
     2041 ccggcatggt gttgaccgtg tcggggccga taagctcttc cacgtagata acatcgcggt
     2101 agttgggatt cttggtggag gtggaggccc agagggggcg ctgcactcgg ccgccgtgcc
     2161 gcttgatgtt ctcccagcgg tcacccttga acacctcaag gaacgcctgg taggccagct
     2221 tgctgttgtt gatggcggtg gtgccaagga ggcccttgat ctgctgctgc ttgtccacgt
     2281 cgggcgtctg cttgagcagg tcttccagcg ccttgtcggc cttggtgtcc acgcggctga
     2341 cgaagaagga ggccaccgag gcgacgtggt ccaccggctt gccttcgcgc actcgccgct
     2401 ccaacgccga gagataggct tccatcacct gccggtgtac gtccaccgag aagatgaggg
     2461 tgatgttgac gttgatgccg cggtagaggc actcctcaat cgccgggatg ccttcatggg
     2521 tggccggtat cttgatgaag atgttcggct tctttatagc atcccaaaac tcctgggcct
     2581 cgtttatcgt gccctgggta tcgtgggcca ggcccggcgc tacctcgatg gagatgtagc
     2641 cgtccccgcc gtcggtgcgg tcgtatacgg ggcgcaggat gtcggcggcg cggctaatat
     2701 cctcgatgat gaggatcttg tagatgtcgg cggagctttt gccctgccgg acgaggtcgt
     2761 ctatcgcggt atcgtagtcg gtggagccgg atatggcctt ctcgaagatg gtcgggttgg
     2821 ccgtgacgcc cacgatgccg tcctcatcga tcatgcgctg caactcgccg gaggtcagca
     2881 acccccgccg tatgttgtcc agccacggac tctgtcccgc ctctaatagt tgttgcatcg
     2941 ggttcttagc catttgtttc tcctaacctg actgttttac aagcctcagt gttatccata
     3001 tctcacatct cgtaccattg tatgtgcggg atttcgagtg tactatcccc cctgtcatcc
     3061 tgagcgtagc gaaggatcta agatgctacc ccttccaaag tgttccactt ttagcaccct
     3121 gccgctcagt gttcgatatc atccctcacc aacttcttta gatccttcgc tgcgctcagg
     3181 atgacagg
//
LOCUS       DASRWZ010000701         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1153966, whole genome shotgun sequence.
ACCESSION   DASRWZ010000701 DASRWZ010000000
VERSION     DASRWZ010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153966"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFH60_11100"
     CDS             complement(<1..415)
                     /locus_tag="VFH60_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET6314374.1"
                     /translation="MSSRYDRTTEDYARPEDIEAILKMRRIAVVGLSSDPYKPSNGVS
                     RYLQRAGYDITPVNPTEREVLGLTAYPTLRDLPEPPELVDVFRRPEYVGEVVEDAIAA
                     GAKAIWIQSGIINYEAARKAREAGLITVMDCCLMVE"
     gene            complement(489..1589)
                     /locus_tag="VFH60_11105"
     CDS             complement(489..1589)
                     /locus_tag="VFH60_11105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6314375.1"
                     /translation="MTSGEPGHVNDMQRYLVEEEAEKWQEGRISRREFVRRATLILGS
                     AVVAGPVLLALGCDPNTPEPTPTAAGASTGSTPQPSPQGTATVDVVAPTVAATSEPTT
                     APTTAPTASGTAGTTGGSPFHVPEDDPNVHAEMVEFPATDGSATLKAYLALPTPVNAR
                     IMTPPGVLVIHENRGLTEHIKDVTRRLARANYAALAVDLLSRQGGSDAHPDEAERTGL
                     LGQNPSEQVISDLSAGLDYLKGRGDVVPDKLGVMGFCFGGGYAWRMATARADLKAAVP
                     YYGPNPPLEDVPKIKAAVLGIYGERDTRITGQVPALEEALKQAGVTYEIKIYEGAEHA
                     FHNDTTARYNPEAARDAWEQTLAWFEKHLRGA"
     gene            complement(1948..>2174)
                     /locus_tag="VFH60_11110"
     CDS             complement(1948..>2174)
                     /locus_tag="VFH60_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314376.1"
                     /translation="EAVRPLLAPGAPFVVIIGESAHAGILVPVPDMTAELGRMAGYEA
                     SEVRVLRTRRSSSHKFGLKESAVVLRRGSE"
BASE COUNT          347 a          716 c          688 g          423 t
ORIGIN      
        1 gctcgaccat gaggcagcag tccatgacgg ttatcaggcc ggcctcgcgg gctttgcggg
       61 cggcctcgta attgatgatg cctgactgga tccagatagc tttcgcgccc gccgcgatgg
      121 cgtcctccac cacttcgcct acgtattcgg ggcggcggaa cacgtccacc agttccggcg
      181 gctccggcag gtcgcgcagg gtggggtatg ccgtcaggcc cagcacctcc cgctcagtgg
      241 ggttgacggg cgtgatgtcg tagcccgccc tctgcaagta acgtgacaca ccattgctcg
      301 gcttgtatgg gtccgaggag aggcccacga ccgcgatgcg gcgcatcttg agtatggcct
      361 cgatgtcctc cggcctcgca tagtcctcgg ttgtccggtc ataacgggaa ctcattgctg
      421 cctcctgacg cctgctggct gcacgtacag tagaaagtcc cgtgccacga tagccgaagc
      481 gtcgtctatc acgcaccgcg caggtgcttc tcgaaccacg ccagcgtttg ctcccaggcg
      541 tcccttgccg cctcggggtt gtagcgcgcc gtggtgtcgt tgtggaaagc gtgctcggcc
      601 ccttcgtata tcttgatctc gtacgtaacg cctgcctgct tgagggcctc ctccaatgcg
      661 ggcacctggc cggtaatgcg ggtatcacgc tccccataaa tgcccagcac cgcggccttg
      721 attttgggca cgtcctcgag cggcgggttg gggccgtagt agggcacagc cgccttcaag
      781 tcggctctcg ccgtagccat gcgccaggcg tagcccccgc cgaagcagaa gcccatcacc
      841 cccagcttgt cgggcaccac gtcgccccgg cccttgaggt agtccagccc tgcggagagg
      901 tcggaaatca cctgctccga tgggttctgg cccagcaagc ccgtgcgctc ggcttcgtcc
      961 gggtgggcgt cgctcccgcc ctgcctggag agcaggtcaa ctgccagcgc ggcatagttt
     1021 gccctcgcca accgtcgcgt cacgtccttg atgtgctccg tcagcccgcg attttcgtgt
     1081 atcaccagca cgccgggcgg ggtcataata cgcgcgttga cgggggtggg cagcgccagg
     1141 taagccttga gcgtggcgct gccgtcggtg gcgggaaact ccaccatctc ggcgtgcacg
     1201 ttggggtcgt cttcgggtac gtggaaaggg ctgcccccgg tggtacctgc cgtacctgat
     1261 gcagtaggtg ccgtggttgg tgccgtggta ggttctgagg tagctgctac cgtaggtgcc
     1321 acgacatcta cggtggcggt gccctggggt gagggctgtg gggtcgaacc cgtggaagcg
     1381 ccagcagcag tcggtgtggg ttcgggcgtg ttggggtcgc aacctaacgc gaggagcacc
     1441 ggccccgcga ccacggcgct acccaggatg agggtggccc ggcgcacgaa ctcacgcctg
     1501 ctgatgcgcc cctcctgcca cttctcggcc tcttcttcga cgaggtaacg ctgcatgtcg
     1561 ttgacatgcc ctggttctcc gctagtcatg acggctgctc ctcggagggg gacccgtcgt
     1621 tgtgcgtgcc acgagcgtca tgaggccagt ataacacacg gcggcagtag tagtcagcgg
     1681 gcggaacgct gcgtccgggc tgtagccgtt ccaggtacgg gcactttaaa taacgccccg
     1741 catgacgccc tgtggctgaa gccacgggct gagaagaggc aaagtcccct acggggactg
     1801 tgtaactaga gggtagtctc cgaaggagac ttcgctactt ctcagcccgt ggcttcagcc
     1861 acagggcgtc atgcggggcg ctccacaacc gcttaccgtc tacccctagc tactcgtcac
     1921 tcgatagctg taattcgcaa ttcgtaacta ctcgctcccc cgcctcagga ccaccgcgct
     1981 ttccttcagc ccaaacttgt gcgacgacga cctacgcgtt cgcagcaccc gcacctcact
     2041 cgcctcgtac cctgccatgc gccccaactc ggcggtcata tccggcacgg ggacgaggat
     2101 gcctgcgtgc gccgattccc cgatgatgac cacaaagggc gcgcctggcg cgagcagcgg
     2161 acggaccgct tcga
//
LOCUS       DASRWZ010000702         1696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1358933, whole genome shotgun sequence.
ACCESSION   DASRWZ010000702 DASRWZ010000000
VERSION     DASRWZ010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358933"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1193
                     /locus_tag="VFH60_11115"
     CDS             213..1193
                     /locus_tag="VFH60_11115"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HET6314377.1"
                     /translation="MAVMLEHMQHTGTLPQQPEDAPAIPPATLPLPGRIGFIGAGKVG
                     TALASLLHARGVNVRAVAGRTTTSSHRMAVAARLAPGTATSRAATVAHSDIVFLTVPD
                     DAIGPLCQEIAASGGWREGQGVVHCSGALSSSILQPAADMGALVASFHPLQTFASTEA
                     AVAHMPGSTFAIEGDALLVEQLDRLALLLDGVPIHLTATQKTLYHAAAVIASNYTVTL
                     AALAADLLVGQGIAPDTNTALHYLMPLLRGTMDNLDALGLPAALTGPIARGDAGTVAR
                     HLAALDQTAPQLAQIYRDLGNSTLPLATKKSRLARETIESLRRALEEKEQ"
     gene            1202..>1696
                     /locus_tag="VFH60_11120"
     CDS             1202..>1696
                     /locus_tag="VFH60_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6314378.1"
                     /translation="MQAIDTLSHSDLTVDDLRQLTNEMVDRMLGLIEDCVDADVTLEV
                     PDPQAHDPEASVEDEVTLPWTLGHVIVHATASAEEAAFLAAELARGVPLHGRSRSEVP
                     WRTVTTVEQCRHRLEESRRMRLATLNVWPDQPYLDNTYTPREDRPPLNCVSRFLNGLR
                     HDKSH"
BASE COUNT          321 a          636 c          465 g          274 t
ORIGIN      
        1 atcactcatc actcatcact catcactcag tcctcactac ttcccttgcg ccacatgcta
       61 taatggacct ggaccacttg gatcccgcac gggaccgaga tggcggacaa gtcactatac
      121 cggccttacg cgtgccaacg gagcgcccgc gtggcctttg tccccattcc atcatgcccc
      181 cgtgctgttg ttgctagcct gggggtattt tcatggccgt aatgcttgag catatgcagc
      241 acaccggcac cctaccgcag cagcccgaag acgcgcctgc catacccccc gcaacgttgc
      301 ccttacccgg tcgtatcggc ttcatcggcg cgggcaaggt ggggacagcc ctggcttccc
      361 tgttgcacgc ccgtggggtc aacgtgcgcg cggtggcggg ccgcaccact accagcagcc
      421 accgcatggc cgtggccgct cgcctggcac cgggcaccgc cacctcccgc gccgctaccg
      481 tggcccactc ggatatagtg ttcctcaccg tgcccgacga cgcgataggg ccgctgtgcc
      541 aggagatcgc ggcctccggc ggctggcgcg agggccaggg cgtggtgcat tgcagcggcg
      601 cgctatcgag tagcatatta cagcccgccg ccgatatggg cgcgctcgtc gcctcattcc
      661 accccttgca gaccttcgcg agcaccgaag ctgccgtcgc ccacatgcca ggctccacct
      721 tcgctatcga gggcgacgcc ctcctggtcg agcagctaga ccgcctcgcc ctgctgctgg
      781 acggcgtacc catccacctt actgccacgc agaagaccct ctaccacgcc gccgccgtga
      841 tcgcctccaa ctacacggtc accctggccg ccctcgccgc cgatctgctg gtgggccagg
      901 gcatagcgcc cgacacgaac accgctctgc attaccttat gcccctcctg cgcggcacga
      961 tggacaacct cgacgcgctg ggacttcccg ccgccctcac cggccccatt gcgcgcggcg
     1021 acgcgggcac cgtggcccgc cacctggccg cactggacca gacagccccc caactggccc
     1081 agatatatcg cgacctcggc aatagcaccc tgcctttagc cacaaaaaag agccgcctgg
     1141 cacgagaaac gatagagtca ttaagacgag cgcttgaaga gaaggagcaa taaacaaccc
     1201 gatgcaagcc atagacactt tgtcgcattc cgacctgaca gtagacgatt tacggcaact
     1261 cactaacgaa atggtagata ggatgctggg acttattgag gactgcgtcg atgcggacgt
     1321 aacattggag gtgccggacc cccaggccca cgaccccgaa gccagcgtag aagacgaagt
     1381 gacgctgccc tggacgctgg gccacgtcat cgtgcatgcc accgcctcag cagaggaggc
     1441 cgctttcctg gccgccgagc tggcgcgcgg ggtgccttta cacggccgct ctcgtagcga
     1501 ggtgccctgg cgcaccgtca ccaccgtcga gcagtgccgc caccgcctcg aagagagtcg
     1561 ccgcatgcgc ctggcaaccc tcaacgtgtg gcccgaccag ccctacctgg acaacaccta
     1621 caccccccgc gaggaccgcc cacccctcaa ctgcgtcagc cgcttcctca acggcctccg
     1681 ccacgacaaa agccac
//
LOCUS       DASRWZ010000703         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_811183, whole genome shotgun sequence.
ACCESSION   DASRWZ010000703 DASRWZ010000000
VERSION     DASRWZ010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811183"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFH60_11125"
     CDS             <1..808
                     /locus_tag="VFH60_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314379.1"
                     /translation="KTYTVQYFERAVFELHTELPAPNNVLLSLLGNFLYKQKYPSGAP
                     NQQPNADLNFQVFNETGKRVGGKFLDYWKSHGGLAQQGFPISEEFTEVSPLNGLPYKV
                     QYFERAVFEYHPENQPPYDVLLSQLGKFRYDAKYGKGGPQPTTPPGQPTAQPTAQPTT
                     PPAASCDLGGGRNGTATPNQVKPGDTVTFEATGFVPGENVSFWFTAPDGSVAGTARPL
                     CCAEPDGKVRFAPLQIPASFADFPGRWALTVQGAISGAGRSAVIPFCVTR"
     gene            1080..>2773
                     /locus_tag="VFH60_11130"
     CDS             1080..>2773
                     /locus_tag="VFH60_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314380.1"
                     /translation="MTRHKSMRRLPYLMLGALALSLIGGWGGAAADTRPKRSDLSPYL
                     TMSAQGRIAEASSLTGSAGNMFAANLQAQAAFANPAFQRVWERTDKPVASGQVQRSWY
                     WGPTANSQGLQEDYAEGAGGKRLVQYFDKSRMEINNLGGDPNSQFYVTNGLLAVELIS
                     GKMQVGNAAYTDRYPADIPLASDADDANAPTYLSFQGVTNTPLGDHPAESKVGTAAIS
                     TIARNGAVGEDATKSSYPRVNYVHFEQATKHNVPQAIWEFLNESGPVYNSATGRNADA
                     RLSEPWFYATGLPISEPYWARVKIAGQQQDVLIQAFERRIVTYAPNGVPGFKVQMGNI
                     GQHYYDWRYKDAGRPTIPPTQPTPVSQPTTTGPQPTATTAPATATATAVPQPQDPNTT
                     CGGIPAPQNMIVRPDCGPLGTTFRFEGSGFEPGEQVGIYVSLPDQSGFDAEFQATAEP
                     DGKVTGIGLGTFNDPEIFPPGIWGITMEGVTTHNKAIGYFKILSPGPTPTPPPAGTPL
                     ACDTSGSVNGTATPPSGRPGTTIFFRATGFSPNEEISYWFTLPNGDVFGTVEPVPPG"
BASE COUNT          565 a          892 c          861 g          455 t
ORIGIN      
        1 caagacttac acggtgcagt atttcgagcg cgcggtgttt gaattgcaca cggagcttcc
       61 cgcccccaat aacgtgctac tgtcgctgct gggcaacttc ctctacaagc agaagtaccc
      121 aagcggcgcg cctaaccagc agccgaacgc cgacctcaac ttccaggtat tcaacgagac
      181 cggcaagcgc gtaggcggca agttcctcga ctactggaag tcgcacggcg ggctggcgca
      241 gcagggcttc cccatttcag aggagttcac agaggtatcg cccctgaacg ggctgcccta
      301 caaggtgcag tacttcgagc gagcggtgtt cgagtaccac cctgagaacc agccaccata
      361 cgacgtgctg ctgtcgcagt tgggcaagtt ccgctacgac gccaagtacg gcaagggcgg
      421 ccctcagccc accacgccgc ccggccagcc tacggcccaa ccgaccgcac agcccaccac
      481 gccgcccgcg gcaagctgcg acctgggtgg cggcaggaac ggcactgcta ccccgaacca
      541 ggtgaagccg ggcgacaccg ttacgttcga ggccaccggc ttcgtaccgg gcgaaaatgt
      601 gtcgttctgg ttcaccgcgc ccgacggctc ggtagcaggc acggcgcgcc cgctgtgctg
      661 cgcggaaccg gacggcaaag tacgcttcgc acccttgcag ataccggcat cgttcgctga
      721 tttccctggg cggtgggcgc tcacggtgca gggtgccata agcggtgcgg gccgttcagc
      781 cgtcataccc ttctgcgtga caaggtagac tgaacaactg agtctggcgt tatctttcgg
      841 gcaagagagg cggcaattcg cctctcttgc ttgatttaac cgaggctttc tggtatagtc
      901 aatcgaacca tatgtcagag actgttctac gggcacgcga ccctatgaga tatggcatgt
      961 atatgctgtg cgaaaggcgc tttatctcag cgcgtattat cgtggtggcc tggggagggt
     1021 ctcgtttgga gcggggctac cgcatcagtt aagatgccat tagcgaaagg aaagttagct
     1081 tgacacgtca caaaagcatg cgccgccttc cgtaccttat gctcggcgcg ctcgcactat
     1141 ccctcatcgg cggctgggga ggggcggcag ccgatacgcg gccaaaaagg agcgacctct
     1201 cgccgtacct caccatgagc gcccagggta ggattgcgga agcctccagc ctgaccggga
     1261 gcgcaggtaa catgttcgcc gcgaacttgc aggcgcaggc ggctttcgct aatcccgcct
     1321 tccagcgcgt gtgggagcgc accgacaagc cggtggcttc ggggcaggtg cagcgctcgt
     1381 ggtactgggg gccgaccgcc aacagccagg gcttgcagga agactacgcg gagggcgcgg
     1441 gcggcaagcg gctggtgcag tacttcgaca agagccgcat ggagatcaac aacctgggcg
     1501 gcgaccccaa cagccagttc tacgtcacga acggcttgct ggcggtggag cttatctccg
     1561 gcaagatgca ggtgggcaac gcggcctaca ccgaccgcta cccagccgac attcccctgg
     1621 cctctgatgc ggatgacgcc aacgcgccga cctacctcag cttccagggc gtaaccaaca
     1681 cgcccctggg cgaccaccca gccgaatcga aggtggggac ggcggctatt tccaccatcg
     1741 cacgcaacgg cgcggtgggt gaggatgcca ccaagtcgag ctatccgagg gtgaattacg
     1801 ttcacttcga gcaggctacc aagcacaacg taccgcaggc catatgggag ttcctgaacg
     1861 agtcgggacc cgtgtacaac tcggctacgg gcaggaatgc cgacgcccgg ctgtcggaac
     1921 cctggttcta cgcgacgggc ctccccatct ccgagccgta ctgggcgcgg gtgaagatag
     1981 cgggccagca gcaggacgtg ctcatccagg cgttcgagcg gcgtatcgtg acctatgcgc
     2041 ccaacggcgt gccgggcttc aaagtgcaga tgggcaatat cggccagcac tactacgatt
     2101 ggcgctacaa ggacgcgggg aggccaacca taccacccac gcagcctacg cccgtgagcc
     2161 agccgacgac caccgggcca cagcccacag cgaccaccgc cccagcgacc gctacagcta
     2221 ccgcagtgcc ccagccccag gacccgaaca ccacttgcgg aggcataccc gcgccgcaaa
     2281 acatgatcgt gcgccccgac tgcgggccgc tcggcaccac gttcaggttt gagggtagcg
     2341 gattcgagcc gggggagcag gtgggaatct acgtgtcgct gcccgaccaa tcgggattcg
     2401 acgcggagtt ccaggcgacc gctgagccag acggcaaggt gacaggcata gggttgggga
     2461 cctttaacga tcctgaaatc ttcccgccgg gcatctgggg catcactatg gaaggtgtga
     2521 cgacgcacaa caaggccata ggctacttca agatactgtc gcctgggccg acgcctacgc
     2581 cgccgcccgc gggcacaccg ctggcctgcg acacgtcggg cagcgtgaac ggcaccgcga
     2641 cgcctcccag cgggcggccc ggcaccacga tattcttcag ggcaaccggc ttctcaccca
     2701 atgaagagat ttcctactgg tttactctgc ccaacggaga tgtgtttgga acggtggagc
     2761 cggtgccgcc ggg
//
LOCUS       DASRWZ010000704         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_674402, whole genome shotgun sequence.
ACCESSION   DASRWZ010000704 DASRWZ010000000
VERSION     DASRWZ010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674402"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..2088)
                     /locus_tag="VFH60_11135"
     CDS             complement(106..2088)
                     /locus_tag="VFH60_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314381.1"
                     /translation="MPSVEQVPYTRIAPWKRTEAINIVAPALASRPNARKTARERVRL
                     VVVFGATLLYACLLLYMLSLSSSSLSPSLGLTAQRDAGGVWRVTSLVPGGLSYDNGAH
                     PGDTVIAAFDATGVALDLKSLPGDTSDFQSAYQLTVLSAGNPGAKPLEISTANQRPDT
                     PLQRWGYALLGIIFIAVGGPVFVKARQRDAARVFYLFCLTTAVALALASAMYLRIDWL
                     QSMLFITLAAWAGSFATFFFKFPVRAGKSQLAHYLILTTVAVGALAVVLGYLWVLLGN
                     YKDYETVRMLTLIYVSACVAAGLGTLVRSLLGERSPEVRQQLFLLLGGTAVAVGPPLL
                     LDLLPHIIVNEAIVSIDLAVLSLGFLPLAFAYAITQHQLLGIRSLVRRSVVYVVLGFT
                     VLVVFALAAAIVSTLMPQGWWGSEFGFLGFGLFVFLIALSFGWAQRRVERLVDRYIYH
                     DAYDYKEALLQFSAQLASEQDLNVLAEQLVERTCRLMNLTCGVLLLASQPQEVDQPDR
                     LPSTLGLDFGDAHSEFDQALTARVGAAAARNNRPQKPEDGLYLEPYASYGSHADTLLE
                     GLRDELSSLGIVLRQPDSTAQMVYFDGTEQRAAFGAAVSSSMQTGKLGSQASFDYEDH
                     AEFESVRSVLGVPLWTRSYFIGVLCLGGKKTGERFT"
     gene            2683..>2888
                     /locus_tag="VFH60_11140"
     CDS             2683..>2888
                     /locus_tag="VFH60_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314382.1"
                     /translation="MSTGTETQTTAQATGTQEGPGYANPDILVSTQWVEDHLNDPNIR
                     RVESDEDVLLYEQGHVPGAVKLDWV"
BASE COUNT          622 a          821 c          968 g          477 t
ORIGIN      
        1 ggtgatagta tcgagtagcg cctgctcgcg ggcctcgtac aattgcgcgt tgtaggtggc
       61 gagggcggcc tggctaccca gcgtgctgag cagcgatatg tcgtcctagg tgaagcgctc
      121 gccggtcttc ttgccgccga ggcagagcac gccgatgaag taggagcggg tccacagggg
      181 cacgcccagg acagagcgca ccgactcgaa ctcggcgtgg tcctcgtaat cgaaggaagc
      241 ctgagaacct agcttcccgg tctgcatgga agaagacacg gccgcaccaa aggccgcgcg
      301 ttgctctgtg ccatcgaaat agaccatttg cgccgtgctg tcaggctggc gcagcacgat
      361 acccaggctg gatagctcgt cgcgcaggcc ctccagcagc gtgtcggcgt gcgacccgta
      421 gctggcgtac ggctccaggt agagtccatc ctcgggcttt tgcgggcggt tgttgcgggc
      481 ggcggccgcg cccacgcggg cggtgagggc ctggtcgaat tcgctgtggg cgtccccaaa
      541 atcgaggccg agagtcgagg gcaagcggtc gggctgatct acttcctgcg gctgagaggc
      601 gagcaagagc acgccgcagg tgaggttcat caggcggcag gtgcgctcca caagctgctc
      661 ggccagcacg ttcaggtcct gctcggaggc gagctgggcg ctgaactgca acagcgcttc
      721 cttgtagtcg taggcgtcgt ggtagatgta gcggtccacc aagcgttcga cgcggcgctg
      781 cgcccagccg aaagacaggg caatgagaaa cacgaagagg ccgaagccca ggaacccgaa
      841 ctcgctaccc caccagccct gcggcatgag ggtgctcact atcgcggcgg ctaacgcgaa
      901 gacgaccagc acggtaaagc cgaggaccac gtacacgacg ctacgccgta ccaggctgcg
      961 gatgccgagc agttggtgct gcgtgatggc ataggcgaag gcgaggggca agaaaccgag
     1021 ggagagcacg gcgaggtcta tgcttacgat ggcctcgttt acgatgatgt ggggcagcag
     1081 gtccagcagc agaggggggc cgaccgcgac ggccgtgcca cccagcaaca ggaatagctg
     1141 ctgtcggact tccggggacc gctcgcccag cagcgacctg accagcgtgc ccagccccgc
     1201 ggccacgcag gcgctcacgt agatgagggt gagcatcctc accgtctcgt agtccttgta
     1261 gttgccgagg agcacccaca ggtagccgag cacgacagcg agggcaccga ccgccaccgt
     1321 ggtcagtatg agatagtgtg ccaattgcga tttgcccgca cgcaccggga acttgaagaa
     1381 gaaagttgcg aacgatcccg cccaggcagc cagcgtgatg aagagcatcg attgcagcca
     1441 gtctatacgc aggtacatgg ccgaggcgag ggcaagggct acggcggtag tgaggcagaa
     1501 gaggtagaag accctggcgg cgtcgcgctg ccgggctttg acgaacacag gcccaccgac
     1561 cgctatgaat atgatcccca gcagggcata accccagcgc tgcaacggtg tgtcggggcg
     1621 ctggttggcg gtgcttatct ccaggggctt cgcgccgggg ttgcccgccg agagcacagt
     1681 aagctggtag gccgattgga agtcgctcgt atctccgggg agcgatttca ggtctagggc
     1741 aacgcctgtc gcgtcgaagg cggcaatgac ggtgtcgccg gggtgcgcgc cgttatcgta
     1801 gctcagcccg ccgggcacga gcgaggttac ccgccacacg ccgccggcgt cccgctgggc
     1861 ggtgaggcct agcgaggggc tgagcgagga gctgctgagg gagagcatgt agagcagcag
     1921 gcacgcgtag agcaaagtcg ccccgaacac cacgaccagg cgcacgcgct cgcgggccgt
     1981 cttgcgcgcg ttggggcgag acgccagggc cggggcgacg atattgatcg cctcggtgcg
     2041 cttccaggga gcaatgcgcg tgtagggcac ctgctcgacg gagggcatgg gctggtgttg
     2101 ccagcgcact tgagtgtctg gagcgatgtt gtgctgaaaa gcacgattat gatgttgcaa
     2161 atgccgctga gagtggtcca taaaaacctg agtgtaacga gccttgaggc atatcgccgg
     2221 actcaaagcc gttcactatg ggctaagcga ccagagagta agaagaagca agtacgtggt
     2281 gcgctatagc atcttcatct aattgttctt aagcacaatt gactgatgcg aagactatta
     2341 tacgtgtctg gttgacgtaa aagcaagaac gaaaagtcct gccggggtgg tactttagtc
     2401 atacctggta ccagaccacc gggccccctg gcccgtgccc gcacgccggg agatgcgtac
     2461 ggaaccgtat aaaaggtcct gtatgcgtta agtaacgttc catcgacaca gaagatagct
     2521 accggcagta gagtggtgtc tcgtgacctg cctaccatga cgaccggcca cattaaaaca
     2581 gcccgaacag tgtataatag gtacgcgact gcgttactct ttggatagca gaccggcaaa
     2641 caaagtagcg tatctaaaaa taccagggaa aaggagcaca ctatgtctac aggcacagag
     2701 acgcaaacta cggcgcaggc caccggcacc caggaaggtc ccggctacgc caatcccgat
     2761 atactcgtgt ccacgcagtg ggtcgaggac cacctgaacg accctaacat caggcgcgtg
     2821 gagtcggacg aggacgtgct gttgtacgag caggggcacg tgccgggcgc ggtcaagctt
     2881 gactgggt
//
LOCUS       DASRWZ010000705         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_331203, whole genome shotgun sequence.
ACCESSION   DASRWZ010000705 DASRWZ010000000
VERSION     DASRWZ010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331203"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..820)
                     /locus_tag="VFH60_11145"
     CDS             complement(143..820)
                     /locus_tag="VFH60_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET6314383.1"
                     /translation="MSRAGEPPVDHSTGLDEDTRRARLLELNVRLRACRRCHAAGYLD
                     ERESVPLARDPDPEASLPRVLLVGQAPGLRATTEYKPFAGITGDKLRGWMEAGGIPRE
                     DFWRKIHFSAVTKCYPGRLPGARGDRVPTPEEQVLCRPWLDGQMALLRPDIVVLVGLL
                     AIQTFMGRVPSLTAVVGTGVIKDGVRYLPLPHPSGVSRWLNEPENIRAVARATGIMRL
                     WVEELGI"
     gene            complement(966..>1556)
                     /locus_tag="VFH60_11150"
     CDS             complement(966..>1556)
                     /locus_tag="VFH60_11150"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET6314384.1"
                     /translation="CEALGIPNLVTHAGAHMGSGEERGLERIASAIERVVGERPGQDV
                     MVLLETTAGQGTSLCYAFEHLRDVRQMLSDEARARVGVCWDTCHLLAAGFDFSDEYRY
                     ERLVESFDRIVGLDTLRAIHMNDSKKGLGSRVDRHEHIGKGALGLEPFRFIMNDPRFE
                     NVPKILETPKEGDKGPDGKPIEMDPINLEALKSLVK"
BASE COUNT          260 a          514 c          485 g          297 t
ORIGIN      
        1 gtgctatagg aaaagggcca catcccggtg taggacgaga ttcttcgcta cgctcagaat
       61 gacaaagggg agcagggtcc caccattgga gcggggtagc attcacgcac cggctaccct
      121 aagccacgtc tctcctcact catcagatcc ctagttcttc cacccacagc cgcataatgc
      181 ctgtggcccg cgccactgcc cggatgttct ccggctcgtt gagccagcgg ctgacgccgg
      241 aggggtgggg cagcggcagg tagcgcacgc cgtccttgat tacgcccgtg cccaccaccg
      301 cggtgagcga gggcacccgg cccatgaaag tctgtatcgc cagcagaccc acgaggacga
      361 ctatgtcggg ccgcaggagt gccatctggc cgtcgagcca ggggcggcag aggacctgct
      421 cttcgggggt gggtacgcgg tcgcccctcg cgccgggcag gcggccgggg tagcacttgg
      481 tgacggcgct aaagtggatt ttacgccaga agtcctctct cggtatgccc ccggcctcca
      541 tccagccgcg cagcttgtca cctgtgatac cggcgaaggg cttgtactcg gtggtggccc
      601 gcaggccggg tgcctggccc accagcagca cgcggggcag cgacgcttcg gggtcagggt
      661 cgcgggccag gggcaccgac tctcgctcat cgaggtagcc cgccgcgtga caccgcctgc
      721 aagcccgcaa gcgcacgttt agctccagca gcctcgcgcg cctggtatcc tcatctaacc
      781 cggtgctatg gtccaccggc ggctctcccg ctcgactcat ggtatcgact ttctagctgg
      841 cgcggacgat ggacgatagg agacgaaaac gcaaagacgc aaagaacgcg caaagatggt
      901 tattgttgtt tggtgctagc cacaagctat cgggatgcca tcgtctatcg ttcatggtcc
      961 atcgtctatt tgaccaggct cttgagggct tccaggttga tggggtccat ctctatgggc
     1021 ttgccgtccg gccccttgtc gccttccttg ggggtctcca gtatcttggg cacgttctcg
     1081 aacctggggt cgttcatgat gaagcggaac ggttcgaggc ccagcgcgcc cttgcctatg
     1141 tgctcgtggc ggtccacgcg gctccctagc ccttttttcg agtcgttcat gtgtatggcg
     1201 cgcagcgtgt cgaggcccac tatcctgtcg aacgactcga ccaggcgctc gtatctgtac
     1261 tcgtcgctga aatcgaagcc cgccgctaac aggtggcagg tgtcccagca gacacctacg
     1321 cgcgcccgcg cctcgtcact gagcatctgg cggacgtcgc gcaggtgctc aaaggcgtag
     1381 cacagggacg tgccctgccc cgccgtcgtc tccagcagca ccatgacgtc ctgtcccggc
     1441 cgctcgccca ctacgcgctc gatagccgag gcgatccgct caagtccgcg ctcctcaccc
     1501 gagcccatgt gcgcgcccgc gtgggtgact aggttgggga tgcccagggc ttcgca
//
LOCUS       DASRWZ010000706         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_811243, whole genome shotgun sequence.
ACCESSION   DASRWZ010000706 DASRWZ010000000
VERSION     DASRWZ010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811243"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..756
                     /locus_tag="VFH60_11155"
     CDS             43..756
                     /locus_tag="VFH60_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314385.1"
                     /translation="MDGFSTGSDAETMQFDAIGADTGYTASGGTGTTDVGQDIGGTVR
                     GTVQQVQETAGNVAGQAQETVGRVADQVRQTASNRLEDQKNRAVEGISNVAEQVREMS
                     LNMQDQGAVAEYMDRAAWQLQRFSDYLQEKDLRDLAIEAEHFARRQPTMFLAGGFLLG
                     LLGARFLKSSARQLQAQGYSSGGSSSSSSVPQVVHFTTQGDNTVEGRSGAGLPMDADG
                     GDTFRSTYVVTPRDGGSED"
     gene            760..1155
                     /locus_tag="VFH60_11160"
     CDS             760..1155
                     /locus_tag="VFH60_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET6314386.1"
                     /translation="MQVNKEDRSIGELFADLAKESSVLVRQEVALAKAEMTQKAGEVG
                     KDIGFLAVGGLVLYAGLLAIIAAIIIVLGTVGLPWWLSALLVGLIVAGVGYFLVQKGL
                     KALKRENLAPRQTIQTLKDDAEWAKEQVR"
     gene            1152..>2293
                     /locus_tag="VFH60_11165"
     CDS             1152..>2293
                     /locus_tag="VFH60_11165"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HET6314387.1"
                     /translation="MRPYGAVDDEGNNEATQGGGAEDSSVVVYRPYTPDDTAPSISDA
                     PTSQYAGPAYDATPQTLQPSFLDATPDPVLEADEAIITDVDAGRDLGQDAGADDWAAR
                     VPINPVTPVPVLPVAPVVPVTHQAPVTSVARALSEDDAGWTAGSGYDAPATGLGDGGD
                     EDVTIPDDPEAIRAGIEQTRAEMSDTINAIQEKLNPQILMDQAKDTLQDAASNILNNA
                     KDTVRDATVGRVEHMVSNVTDTGGNFVQMVKDNPIPAALVGLGLGWLFTRNSGSSNKG
                     RDYRAYSNYRDYPDYTDRGYPGAGSYQPQSYGRQAYGSYSGGQGDGDGLVDQVSGRIK
                     QNPVPAALAAAGLGWLLMGGGSNTQSSETSYARYGGGGYSGQRGYG"
BASE COUNT          473 a          700 c          785 g          335 t
ORIGIN      
        1 acagttatgg accagagaga ccaatattcc gagggaaatg gcgtggacgg attctccacg
       61 ggcagcgatg ccgagaccat gcaattcgac gcaatcggcg cggacaccgg gtataccgct
      121 tcgggcggca ccggcacgac agacgtcgga caggacatag gtggcacggt gcggggcacg
      181 gtgcagcagg tgcaggagac cgccggtaac gtggcggggc aggcccagga gacggtgggc
      241 cgggttgccg accaggtgcg ccagaccgcc tccaaccgcc ttgaagacca gaagaaccgc
      301 gccgtcgaag gcatctcgaa cgtagccgaa caggtgcgcg agatgagcct caacatgcag
      361 gaccagggcg cggtggccga atatatggac agggccgcgt ggcagctaca gagattctcc
      421 gactacctgc aagagaaaga cctgcgcgac ctggcgattg aagcggagca tttcgcccgc
      481 cgccagccga ccatgttcct ggccggtggg ttcttgctcg gcctgctggg cgcccgcttc
      541 ctgaagagtt ccgcccgcca actgcaagcg caaggttatt cgagtggcgg aagtagcagt
      601 agcagttccg tcccgcaggt cgtccacttc accacgcagg gcgacaacac agtcgaggga
      661 cgctcagggg ccggacttcc gatggacgcg gatggcggtg acaccttcag gtccacctac
      721 gtggtcactc ctcgtgacgg cggctcggag gactaagcga tgcaagtaaa caaagaagac
      781 cgctcgatag gagagctttt cgcggacctt gccaaggaat ctagcgtgct ggtacgccag
      841 gaggtggctc tcgccaaggc agagatgacc cagaaggcgg gcgaggtcgg caaagacatc
      901 ggcttcctgg ctgtgggcgg cctcgtactc tacgcggggc tgctggcgat catcgcggcg
      961 atcatcatcg tgctgggcac ggtaggcttg ccgtggtggc tgtcggccct gcttgtgggc
     1021 ctgatcgtgg ccggtgtggg ctacttcctc gtgcagaagg gcctcaaggc gctcaagcgg
     1081 gaaaatctcg cgccgcgcca gactattcaa acgctgaagg acgatgctga gtgggcaaag
     1141 gagcaggtga gatgagacca tacggagctg ttgacgacga gggtaacaac gaggccaccc
     1201 agggcggcgg tgccgaggac tcgagcgtag tggtctaccg cccatacaca cctgacgaca
     1261 ccgcaccctc catttcggac gcgcccacga gccagtacgc gggacctgca tatgatgcga
     1321 cgcctcagac gttgcagccc tcgttcctgg acgccacgcc cgacccggtg ctggaagcgg
     1381 acgaggccat aatcacggac gtggacgcgg gccgcgacct ggggcaagac gccggggccg
     1441 acgactgggc ggcgcgggtg cctataaacc ccgtgacccc ggtgccggta cttcccgtcg
     1501 ccccagtcgt gcctgttact catcaagcgc cggtaacgtc tgtggcgagg gcgctcagtg
     1561 aagacgatgc cgggtggacc gcgggctcgg gctacgatgc cccggcgacc ggcctcggcg
     1621 atggcgggga cgaggacgtg actatccctg acgaccctga ggctatcagg gccgggatag
     1681 agcagacccg cgccgagatg agcgatacga tcaacgccat ccaggagaaa ttgaacccgc
     1741 agatattgat ggaccaggcc aaggacacgc tccaggacgc cgcgtccaac atcctcaaca
     1801 atgccaaaga caccgtgcga gacgctacgg tggggagggt tgaacacatg gtgagcaacg
     1861 taacggacac ggggggcaac ttcgtccaga tggtgaagga caacccgatc ccggcagccc
     1921 tggtggggct tggcctgggc tggctcttca cgaggaacag cggctcgtcc aacaagggcc
     1981 gcgactaccg cgcctactct aactacaggg actaccccga ctacacggac cgcggttacc
     2041 cgggcgcggg gagctaccag ccccagagct acggcaggca ggcttacggc agctacagcg
     2101 gcggccaggg agacggcgac gggctcgtag accaggtatc gggccgcatc aagcagaacc
     2161 cggtgccggc cgcgcttgca gccgccggcc ttggctggct gctcatgggc gggggcagca
     2221 acacccagtc gagcgagacc tcctacgctc gctacggcgg cggtggctat agcggccagc
     2281 gcgggtatgg cag
//
LOCUS       DASRWZ010000707         5200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_194986, whole genome shotgun sequence.
ACCESSION   DASRWZ010000707 DASRWZ010000000
VERSION     DASRWZ010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194986"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="VFH60_11170"
     CDS             <1..1601
                     /locus_tag="VFH60_11170"
                     /inference="COORDINATES: protein motif:HMM:NF022812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HET6314388.1"
                     /translation="DPNAAVPALVQAEVNADLAQIDAHQGLSPSAVFGIGNPDEYMED
                     FSQYVPRGHYTRSEDLKRYFRAMMWYGRMTFRLQSVEETKSALLVTQALQTAKNGNET
                     AAQLWATIYEPTSFFVGGSDDLTYLDYAPLMQQALGPDPKAVADNAKIAQFQELAKTL
                     AGPKVNSMFVLIDEDKEAVTKGLRMMGQRFTLDAYVFGQLIWRNVGNPNGPESEKRWL
                     PKGLDVPAAFGSQEALSILEGMGETKYMSYTLQLNKVRGEISGLADSQWTENLYWSWL
                     YSFRPLLQPKAANSGYPSFMTNSAWTRKGLNTVLGSWTELKHDTILYAKQVMAEMGGG
                     PPETIKGYVEPEPEFYARIAALIGMTRDGLLARGLLEKTEDEFARSEYNTLNDLYNLA
                     IDLKRISEKELQGQALTEDEYALIQYYGGRLENSTLAASDEDENGQPGGDLNDQDAAL
                     VADVATGGPGGDLALEEGTGRIMEIYVVVPVEGRLVLARGGIYSQYEFVQPSSNRLTD
                     EQWRAMVESGRLPPLGEWKTFIAK"
     gene            complement(1695..3056)
                     /gene="nhaA"
                     /locus_tag="VFH60_11175"
     CDS             complement(1695..3056)
                     /gene="nhaA"
                     /locus_tag="VFH60_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET6314389.1"
                     /translation="MPRLRIPLATHWTETPVGRAVRPMQEFVHSSTAGGIVLLFAAVV
                     ALVLANSPLRAPYEEVLNTYLDFQIGTFQLHQTVLHWINDGLMAIFFFLVGLEIKREI
                     WAGELSNVRAALLPIVAALGVAIVPALVYAAINLGGPGNAGWGIPMATDIAFALGVLA
                     LVGSHLPFTLKILLTAIAIIDDLIAVLVIAFFYSGSLDFSALGIGFALLGVLLAMNAI
                     GIRNIPLYALIGVLVWVAFLQSGVHATIAGVLVALMIPVRTRINQVTFLQRARSILDS
                     FEDSSKREPYSILQDQEQQSAVIELEELSEAVQAPLQKMEHTLHNWVAFIIMPLFALA
                     NAGVALSAEALRGETLPVALGILAGLLIGKPLGLFGATWLAVRAGLVTLPQSVTWRHI
                     AGLACLGGIGFTMSLFVATLAFGGDPLLETAKLGILAASLIAGTAGFLLLKTTPAGPD
                     PTP"
     gene            complement(3122..>5200)
                     /locus_tag="VFH60_11180"
     CDS             complement(3122..>5200)
                     /locus_tag="VFH60_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET6314390.1"
                     /translation="AAHPVAFAVGGLSLAALALRLFGISDNLPYTSNPDEPAVADRAL
                     GILQTGDYNPHYFVYPNLYTYMQAAVFLPLFFLLVSSGSLETLDQVVPTDFYLWGRLL
                     TALLGTLTVPVVFLAARRLYGTTTALIAAAFMATNSVHLVYSQLITTDVPATFFSALS
                     LLTIAWLLPPSEAAHEANPTPHSPLPSRRLYLIAGVAMGLAVGTKYNSALVVLPFLLA
                     HAYAVAAQTPALGHRLRAFFGGRLWLALASMAGAFLLTTPFALFDLPNFLNDIASVLA
                     HYRFGHLGFEGDENWRFYFGTFLQSDFLPTLLVLVGVIVALARRNRADLLLLSFPLVF
                     YLSMSSYRVNFTRNMLPIIPFTSILAAMSLALAWNALMSYLAKQPSAAQRPITRLAPW
                     LLGVIVALGLLPQTAAALQRGYRQTQPDTRLRAAQWLDANTRPGTKLWLEFDTPKLTT
                     GRYLIGSGQHVTERPMDWYVANRYEYLVLSAGAYKPVVYDRPESNPPLRDAYLRFFDE
                     NRANLVVSFETNKVDHPGPTIQIYRTGYTPPRSAQDVRSQHPVNAHYREIDPNSGLIT
                     LIGADFPLDIQPASSLPITLYWTTDRPLNTNYTVFVHLVDASGNKLSQIDVAPRSGTY
                     PTSLWPSNEVVVDELTLSLPDSLPPGEYSLRIGLYLQQGAEAHTAFALTNAPPGSGGD
                     YVLLGPLTAR"
BASE COUNT          990 a         1224 c         2109 g          877 t
ORIGIN      
        1 tcgacccgaa cgccgcggtg cccgcgctgg tgcaggccga ggtgaacgcg gacctggcgc
       61 agatagacgc gcaccagggg ttgtcgccgt cggcggtgtt cgggataggc aaccctgatg
      121 agtacatgga ggacttcagc cagtacgtgc cgagggggca ttacactcgt agcgaggacc
      181 tgaagaggta cttcagggcg atgatgtggt acgggcggat gacgttccgg ctgcagtcgg
      241 tggaggagac gaaatcggcg ctgctggtga cgcaggcttt gcagacggcg aagaacggca
      301 acgaaacggc ggcgcagtta tgggccacga tttacgagcc tacttccttc ttcgtgggcg
      361 ggtcggacga cctgacttac ctcgactacg cgccgctgat gcagcaggcc ttaggccccg
      421 acccgaaggc ggtggcagac aacgctaaga tcgcgcagtt ccaggagctt gccaaaacgc
      481 tggcgggtcc gaaggtcaac tcgatgttcg tgttgattga cgaggacaag gaagcggtga
      541 ccaagggcct gcgcatgatg gggcagcggt tcacgctgga cgcttacgtg ttcggacagc
      601 ttatctggcg caacgtgggc aacccgaacg gccctgagtc ggagaagcgg tggctgccca
      661 aggggctgga cgtgcctgcg gctttcgggt cgcaggaggc gctgagcata cttgagggga
      721 tgggcgagac gaagtacatg agctacacct tgcagctaaa caaggtgcgt ggggagatta
      781 gcgggctggc cgactcgcag tggacggaga atttgtactg gtcgtggctg tacagcttcc
      841 ggccgctgtt gcagcccaag gcggcgaata gcggataccc tagcttcatg acgaactcgg
      901 cgtggacgcg taagggcttg aacacggtgc tgggtagctg gaccgagctg aagcacgaca
      961 cgatcctgta cgccaagcag gtgatggcgg agatgggcgg cgggcctcct gagacgatca
     1021 aggggtatgt agagccggag ccggagttct acgctcgtat cgcggcgctg atcggcatga
     1081 cgcgggacgg gctgctggct cgcgggctgc tggagaagac tgaggacgag ttcgcgagga
     1141 gcgagtacaa cacgctcaac gacctgtaca acctggcgat agacctgaag cgcataagcg
     1201 agaaggagtt gcaggggcag gcgctgacgg aggacgagta cgcgctcatc cagtactatg
     1261 gtggccggtt ggagaacagt actttagccg cctcagacga ggacgagaac ggccagcctg
     1321 gcggcgacct gaacgaccag gacgcggcgc tggtggcgga cgtggcgacg ggcgggccgg
     1381 gaggcgacct ggcgctggaa gagggcacgg ggcgcatcat ggagatatac gtagtggtgc
     1441 cggtggaggg caggctggtg ctggcgaggg gaggcatcta cagccagtac gagttcgtgc
     1501 agccttcgag caaccgcctg accgacgagc agtggcgggc gatggtggag agcggcagac
     1561 tgcccccgct tggggagtgg aagacgttca tcgctaagta ggagacgaaa acgcaaagac
     1621 gcaaagggcg cgcaaagatt agaatagtcg ttcttgcaag ggcaggcttg tcgctttgac
     1681 gggcctgccc tttgctatgg agttggatct gggccggcgg gggtggtttt gaggagcagg
     1741 aagcctgcgg taccggcgat gagggaggcg gcgaggatgc ctagcttggc ggtttctagg
     1801 agtgggtcgc cgccgaaggc gagggtggcg acgaagaggg acatggtgaa gccgataccg
     1861 ccgaggcagg ccagcccggc tatgtgccgc caggttacgg actgggggag ggtgacgagt
     1921 cctgctcgca cggccagcca ggtagcgccg aagaggccga ggggcttgcc gatgagcagg
     1981 ccggccagga tgccgagggc cacgggcaag gtctcgccgc gcagggcttc ggcgctgaga
     2041 gctaccccgg cgttggcgag ggcgaacagg ggcatgatga tgaaggctac ccagttatgg
     2101 agggtatgct ccatcttctg caggggggct tgtacggcct cggatagctc ttctagctcg
     2161 atgacggcgg actgttgctc ctggtcctgg aggatggagt agggttctcg cttgctgctg
     2221 tcctcgaagc tgtcgaggat ggagcgggcg cgttggagga aggttacctg gttgatgcgg
     2281 gtgcggacgg gtatcatgag ggccacaagc acgcccgcga tggtggcatg gacgcccgat
     2341 tgaagaaacg ctacccacac cagcacacct atcagggcgt agagggggat gtttcggatg
     2401 ccgatggcgt tcatggcaag gagcacgccg aggagggcga aaccgatgcc tagcgcgctg
     2461 aagtcgaggc tgccggagta gaagaaggct atcacgagca cggctatgag gtcgtctatg
     2521 atggcgatgg cggtgagcag tatcttgagg gtgaagggca ggtggctgcc taccagggcg
     2581 aggactccga gggcgaaggc tatgtcggtt gccattggga taccccagcc cgcgttgccc
     2641 ggtccgccaa ggttgatggc tgcatagaca agggcgggga cgatggccac accgagggcg
     2701 gctactatgg gcaggagggc ggcgcggacg ttgctcaact cgcccgccca gatttcgcgc
     2761 tttatttcga ggccgaccag gaagaagaat atagccatga ggccgtcgtt gatccagtgg
     2821 agtacggtct ggtgtagctg gaatgtgcca atttggaagt ccaggtaggt attgaggacc
     2881 tcctcatagg gggcgcgcag gggtgagttg gccagcacga gggccacgac ggcggcgaag
     2941 agcagcacta tgccgcccgc ggtggagctg tgcacgaact cctgcatggg cctgacggcg
     3001 cggcctacgg gggtctcggt ccagtgggtt gcgagtggga tccttagtct tggcatatca
     3061 cgggtattat acgggactac tactttgagg ctcaacgccg cagaagatga gggatgcgct
     3121 gctagcgtgc ggtgaggggg ccgaggagga cgtagtcgcc gccggagccg ggtggggcgt
     3181 tggtgagggc gaaggcggtg tgggcttcgg cgccctgttg caggtagagg ccgatgcgta
     3241 ggctgtactc gccggggggc aggctgtcgg ggagggagag ggttagctcg tcaacgacca
     3301 cttcgttgga gggccagagg gaggttgggt aggtgccgct gcggggggcc acgtcgatct
     3361 gagagagttt gttgccggaa gcgtctacga ggtgcacaaa gacggtgtag ttggtgttga
     3421 gggggcggtc tgtggtccag tagagggtga tgggtaagga gctagcgggc tggatgtcga
     3481 gtgggaagtc ggcaccgatg agggtgatga ggccgctatt tgggtcgatt tcgcggtagt
     3541 gggcgtttac ggggtgttgt gagcgcacgt cctgggccga cctgggggga gtgtagccgg
     3601 tgcggtagat ctggatcgtg ggacctgggt gatctacttt gttggtctcg aagctgacga
     3661 caaggttagc gcggttttcg tcgaagaagc gcaggtaggc atcacggagg ggcgggttgg
     3721 actcggggcg gtcgtagacg acgggcttgt aggccccggc gctgagtacg aggtattcgt
     3781 agcggttggc gacgtaccag tccatcgggc gttcggtgac gtgctggccg gagcctatga
     3841 ggtagcggcc cgtcgtgagc ttgggggtgt cgaactcgag ccagagcttg gtgccggggc
     3901 gggtgttggc gtctagccac tgggcggcgc ggaggcgggt gtcgggttgg gtctggcggt
     3961 agccgcgttg gagggcggcg gcggtttgcg gtaggaggcc gagggccacg ataacgccga
     4021 gcagccaggg tgccagccgg gtgatgggcc gttgtgcggc gctgggctgc tttgcaaggt
     4081 acgacatgag ggcgttccag gcgagggcga gggacatggc ggcgaggatg gaggtgaagg
     4141 ggatgatggg cagcatgttg cgggtgaagt tgacccgata gctgctcatg gagaggtaga
     4201 agacgagggg gaagctgagc agcagcaggt cggcgcggtt gcggcgggcg agggcgacta
     4261 ttacgcccac gaggacgagg agggttggca ggaaatcgct ttgtaggaag gtgccgaagt
     4321 agaagcgcca gttctcgtcg ccctcgaagc cgagatggcc gaagcggtag tgggcgagca
     4381 cggaggcgat gtcgttcagg aagttaggca ggtcgaagag ggcgaaggga gtggtaagca
     4441 ggaaggcccc ggccatgctc gcgagggcca gccagagacg accgccgaag aaggcccgca
     4501 gccggtggcc cagggcgggt gtttgggcgg ctacggcgta agcgtgggcc aggaggaagg
     4561 ggagcacgac gagggcgctg ttgtactttg tgccgacggc gaggcccata gctacgcccg
     4621 cgatgaggta gaggcggcgg gagggtagcg gactgtgcgg ggttgggtta gcctcgtgtg
     4681 cggcctcgga gggtggcagc agccaggcaa tcgtgagaag ggagagggcg ctgaagaagg
     4741 tggcgggaac gtcggtggtg atgagctggg agtagacgag gtgcacgctg ttggtagcca
     4801 tgaaggcggc ggcgataagc gcggtggtgg tgccgtagag cctgcgggcg gccaggaaca
     4861 cgacgggcac ggtgagggtg ccgaggaggg cggtcaggag gcggccccag aggtagaagt
     4921 cggtgggcac tacctggtcg agcgtctcca gggagcctga ggagacgagg aggaagaaga
     4981 gcggcaggaa gacggcggcc tgcatgtagg tgtagaggtt ggggtagacg aagtagtggg
     5041 ggttgtagtc gccggtctgg aggatgccga gggcgcggtc ggccacggca ggttcgtcgg
     5101 ggttgctggt gtagggcagg ttgtcgctga tgccgaacag ccgaagggcg agggctgcca
     5161 gggagaggcc gccgacggca aatgccacgg ggtgcgctgc
//
LOCUS       DASRWZ010000708         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_263064, whole genome shotgun sequence.
ACCESSION   DASRWZ010000708 DASRWZ010000000
VERSION     DASRWZ010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263064"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..2027
                     /locus_tag="VFH60_11185"
     CDS             72..2027
                     /locus_tag="VFH60_11185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET6314391.1"
                     /translation="MSNPQVAPYGSWKSPITTDAIVAGSLSLGQVAVDGGDVYWSEGR
                     PTERGRVAIMRRTPDGEVTEVTPPEFYVRSKVHEYGGGAYTVHKGTVYFSNYHDGRLY
                     RQEPGGRPQPISPAGVDFRYADFVVDEARGRLISVREDHTVGGPEAINSLVSLPVSGE
                     GHGGESQQTVLLSGNDFYSNPRLSPDGASLAWLAWDHPNMPWDGTNLWVGDFDEGGLV
                     VNKRQVAGGPDESVFQPEWSPDGTLYFVSDRTGWWNLYRLRDGEAEPLHPMEAEFGRP
                     QWVFGTGTYGFEHAGRIICAYAEKGVWKLTSLDTETLEFAPIDTPYTELGTSLRVLPG
                     LVVTDAKSPTEPPSIVQVDLDSGHVTVLRRSSSLEVDPGYLSIPESTEFPTEQGLTAY
                     AFFYPPTNRDYRAPEGEKPPLIVISHGGPTSQTAATRDLRIQFWTSRGFGVLDVNYGG
                     STGFGTEYRRRLNGQWGVVDVDDCANGARYLVERGDADPDRLIIRGGSAGGYTTLCAL
                     TFRDLFRAGASYYGICDLEALDEESHKFEARYNHSMIGPYPEQKELYRQRSPIHHTER
                     LNCPIIFFQGLEDKAVPPRQSEMMVEALRRKGLPVAYLAFEGEQHGFRKAETIKRTLE
                     AELCFYSRIFGFDLGEAVEPVEIENLG"
BASE COUNT          458 a          781 c          801 g          411 t
ORIGIN      
        1 tagactgcgg tggggtgcct gctgaggccg gattgctgta tcatgtcgga aaagacgcag
       61 gaggcacccc tttgtctaac ccacaagtgg ccccgtatgg gtcgtggaag tcgcctatca
      121 ctactgacgc tatcgtggcg gggtcgctgt cgctggggca ggtcgcagtg gacggcggcg
      181 acgtttattg gagcgagggg cggcccaccg agagggggag ggtcgccatc atgcgccgca
      241 cgcccgacgg agaggtgact gaggtcaccc cgcctgagtt ctacgtgcgc agcaaggtgc
      301 acgagtacgg cggcggcgct tacaccgtac acaaaggcac ggtctacttc tccaactacc
      361 acgatggccg cctctaccgg caggagcctg ggggccggcc ccagcccatc tcccccgccg
      421 gcgtggactt ccgatatgcc gacttcgtgg tggacgaggc acgcggccgc ctcatcagcg
      481 tgcgcgagga ccacaccgtc ggcgggcctg aagcgatcaa cagcctcgtg agcctgccgg
      541 tgagcggcga ggggcacggt ggcgagagcc agcagacggt cttactctcc ggcaacgatt
      601 tctactccaa cccgcgcctc agccccgacg gcgccagcct ggcgtggcta gcctgggacc
      661 acccgaacat gccgtgggac ggcacaaacc tctgggtggg cgacttcgat gagggcggcc
      721 tggtggtcaa caagcggcag gtggcgggcg ggccggacga gtcggtgttc cagcctgagt
      781 ggtcgcccga cggcacgctc tacttcgtgt cggaccgcac cggctggtgg aacctctacc
      841 gcctgcggga cggtgaggcg gaaccgctac accctatgga ggccgagttc gggaggcccc
      901 agtgggtgtt cggcaccggc acctacggct tcgagcacgc cggccgcatc atctgcgcct
      961 acgccgagaa aggggtgtgg aagctgacta gcctcgatac cgagacgctg gagttcgccc
     1021 ccatcgacac gccctatacg gagcttggca cttcgctgag ggtgctgccc ggcctggtcg
     1081 tcaccgatgc aaagtccccc accgaaccgc cctccatcgt gcaggtggac ctggactccg
     1141 ggcatgtcac agtgttgcgc cgctccagca gcctggaggt ggaccccggc tatctctcca
     1201 tccccgagtc gactgagttc cccacggagc aggggctgac cgcttatgcc ttcttctacc
     1261 cgcccacgaa cagggactac cgcgcaccgg agggagaaaa gcccccgctg attgtcatca
     1321 gccacggcgg gccgacctcg cagacggcgg ccacccgcga cctgcgcata cagttctgga
     1381 cgagccgggg cttcggggtg ctcgacgtga attacggggg cagcaccggc ttcggcaccg
     1441 agtaccgccg caggctcaac gggcagtggg gcgtggtgga cgtggacgac tgcgccaacg
     1501 gggcgcgcta cctcgtggag cgaggggatg cggaccccga ccgcctcatc atccggggcg
     1561 gcagcgcggg cggctacact accctgtgcg ccctcacctt ccgcgacctg ttcagggcgg
     1621 gcgcgagcta ctacggcatc tgcgacctcg aagccctcga tgaagaatcg cacaagttcg
     1681 aggcgcgcta caaccacagc atgatagggc cgtaccctga gcaaaaggag ctttacaggc
     1741 aacgctcgcc catacatcac accgagcggc tgaactgccc catcatcttc ttccagggcc
     1801 tcgaagacaa agcggtgcct ccacgccagt cggagatgat ggtggaggcg ctacggcgca
     1861 agggcctacc ggtggcctat ctcgcgttcg agggcgagca acacggcttc cgcaaggccg
     1921 agacgatcaa gcgcaccttg gaagccgagc tatgcttcta ctcccgcatc ttcggcttcg
     1981 acctggggga ggccgtcgag ccggtggaga tagagaacct ggggtgacga gtgacgcgta
     2041 acgcgtaaca cgtaacgcgt aaagcgtaaa gcgtaatgcg taacgggctt gctcacgtgg
     2101 gtgcgcgaat acgacaccgt tgctattaca ggaccaaacc ccgcctttgt cattctgagc
     2161 gtagcgaaga atctcgtcct acaccgggat ggtccccttt tctcgtgcgg ctcttgttgc
     2221 catgagaaag gtgcgacctg ggtgctgtag gacgagattc ttcgctacgc tcagaatgac
     2281 aaaggcgggg cttggtccca ccacagaagc agtctgacat tcacggacca acgtagcaag
     2341 cggcgttacg cgttacgtgt cacgcgtaac gtgtcactcg tcacgctcta caggcacttg
     2401 cgaagaagag gcgtttggtc cttgacggcg ttgtaggggc cgacctggct c
//
LOCUS       DASRWZ010000709         1862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1154382, whole genome shotgun sequence.
ACCESSION   DASRWZ010000709 DASRWZ010000000
VERSION     DASRWZ010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154382"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1577
                     /locus_tag="VFH60_11190"
     CDS             210..1577
                     /locus_tag="VFH60_11190"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6314392.1"
                     /translation="MSDNRAADKPAHKLSTNFWLLWTGQAISALGSSFTGFALPLLVY
                     NLTGSVIYLAYATAAHFIPYLLFGLFIGAWSDRTDRKRLMIWTDVGRALGIASVPLLA
                     TLGMLEVWWIFVVLFVNSLFSIAFDAAKFAAVASLVDKDELVTANGRIQASYSTMTIL
                     GPLAAGVLVAVIPIYDLLVIDAMSYVVSTASLVMITAAFNAPGKPRGTSIRQDVVEGL
                     RYVFGHPVLRNISIMMALVNFAGTTVYAQRVLYAKEQLSASDSEVALLAAAGSFGVIV
                     LSLIAGKLRKRLPFSKVALGALMASGLATIGLAFTTWYWLGLVIWSVFSGLGIMFNIN
                     TNSLRQAIAPNHMLGRVVTIAGVLAWSANPLGAFVGGWLIERTQNVGLVYGGIGVLTV
                     LIPVLFAFGPLGHAERYLPQNNPDEQPASQPPPEPEPRQEQIVQVAAEENEADRPREK
                     VGSGH"
     gene            complement(1683..>1862)
                     /locus_tag="VFH60_11195"
     CDS             complement(1683..>1862)
                     /locus_tag="VFH60_11195"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET6314393.1"
                     /translation="GLNRVQAVVLPDNLGSMRVLEKAGMQREGLLRKYQYVLGDYWDV
                     YMYGLLRSDWEASGG"
BASE COUNT          322 a          624 c          543 g          373 t
ORIGIN      
        1 caggggagga actttgcaga tactccccag tctccggagg agacttcgct acttgccagc
       61 ccgccccttt agtggcaggg cacacccaca gggcgtcccg cgggcgcacc aaccgtctcc
      121 ccacccgctg taacctctct cctcccaaaa ccgttgtgta taatggccga accacgtttc
      181 cacactccat cccgcatcga gggtgagaca tgtccgacaa ccgcgccgca gacaagcccg
      241 cgcacaagct aagcaccaac ttctggctgc tgtggaccgg gcaggctatc tccgcgctgg
      301 gcagctcttt caccggcttc gccctgcccc tgctggtcta caatctcact ggctccgtga
      361 tctacctggc ctacgcgacc gctgcccact tcatccccta cctgctgttc ggcctcttca
      421 taggggcatg gtccgaccgc accgaccgca agcggctcat gatctggact gatgtcgggc
      481 gcgccctggg catagcctcc gtgccgctgc tggccacact cggtatgctg gaagtgtggt
      541 ggattttcgt ggtgctgttc gtcaattcgc tcttcagcat cgccttcgac gccgccaagt
      601 tcgcagccgt ggcgagcctg gtggacaaag acgaactcgt caccgccaat ggtcgcatcc
      661 aggccagcta ttccaccatg acaatcctcg ggcctttggc ggcgggcgtt ttagtggctg
      721 tgatacccat ttacgacctg ttggtgatag atgcgatgtc ttacgtggtg tccaccgcct
      781 cgctcgtcat gatcacggcg gccttcaacg cgcccggcaa gccacgcggc accagcatcc
      841 gtcaggatgt ggtcgagggc ctgcgctacg tgttcgggca cccggtgctg cgcaatatct
      901 ccatcatgat ggcgttggtg aacttcgccg gcaccaccgt ctacgcccaa cgggtgctct
      961 acgccaagga gcagctaagc gcgagcgact ccgaggtggc gctcctggcg gcggcgggca
     1021 gcttcggggt gatagtgctc tcgctcatcg cgggcaagct gcgcaagagg ctgcccttca
     1081 gcaaggtagc cctgggcgcg ctcatggcga gtggtcttgc cacgattggc ctggcattta
     1141 ccacgtggta ctggctgggc ctggtcatct ggtcggtgtt ctccggcctc ggtattatgt
     1201 tcaacatcaa cacgaacagc ctgcgccagg ccatcgctcc taaccacatg ctggggcgcg
     1261 tggtaaccat cgcgggggtg ctggcgtggt cggctaaccc gcttggcgct tttgttgggg
     1321 gttggctcat cgagcggacg cagaacgtgg ggctggtgta cgggggaata ggcgtgctaa
     1381 cggtcctcat cccggtgctg ttcgccttcg gcccgctggg gcacgccgag cgatacctgc
     1441 cccagaacaa ccctgatgag cagcctgcat ctcaaccccc gcctgagcct gaaccgcgcc
     1501 aggagcaaat agtacaggta gccgccgagg aaaatgaggc cgaccggccc cgcgagaaag
     1561 tgggctcagg ccactgagct tgccgggtgc ttggtccttc ttggaggctt ggggaggctt
     1621 atcctgccag gtgcgccatg cagggctagc gcagggcgtc tcccgcatag cctgacgaca
     1681 ccctaccctc ctgacgcctc ccaatcgctt ctgagcaggc catacatgta cacgtcccag
     1741 tagtcgccaa gcacgtactg gtacttcctt agcaaaccct cccgctgcat acctgccttc
     1801 tccagcaccc gcatggaacc taaattgtcc ggcagcacaa ccgcctgcac ccggttgagg
     1861 cc
//
LOCUS       DASRWZ010000710         4211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1291713, whole genome shotgun sequence.
ACCESSION   DASRWZ010000710 DASRWZ010000000
VERSION     DASRWZ010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291713"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFH60_11200"
     CDS             complement(<1..195)
                     /locus_tag="VFH60_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314394.1"
                     /translation="MSSSTGYPTTSNVLPVTLGDYDSCGHAVQFYDDDHFLLDALGRF
                     VGSALEAGDAAIVIATEAHRD"
     gene            complement(444..1721)
                     /locus_tag="VFH60_11205"
     CDS             complement(444..1721)
                     /locus_tag="VFH60_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6314395.1"
                     /translation="MIGNPTNWAGNYTYRAAMLHFPETVEQVQELVSGASKLKALGSR
                     HSFNHVADSPGDLILLDRLERVFVLDRERNTVTIDAGARYGRFCEELHREGYALRNMA
                     SLPHISVAGACATATHGSGDRNGNLATAVAAMELVTADGTVVVLSRDERGAGGEQFRG
                     AVVGLGGLGVVTKLSLDVVPAFDMRQDVYEDLPFAQLEAHFDEITSSAYSVSLLLNWR
                     DARVNQVWLKRPVTDGATFELPPEFFGAAPAPTHRHPIPGFSAEPCTEQMGIRGAWYA
                     RLPHFKMGFTPSSGEELQTEYLLPRRHGMAAFRAIYSLREHVVPLLQISEVRTVAADD
                     LWLSPCYGQDCVAIHFTWEKDWEAFSLVLPLVEEQLAPLDARPHWGKLFATSPERVQS
                     LYPKLPDFQRLLRTYDPQGKFRNAFLDVYIFGE"
     gene            complement(1937..2455)
                     /locus_tag="VFH60_11210"
     CDS             complement(1937..2455)
                     /locus_tag="VFH60_11210"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET6314396.1"
                     /translation="MDYDLTELIRDIPNFPVQGILFRDITTLLKVGPAFQQVVDQFCD
                     KLAPLNATKVVAIESRGFIFGAPIAYRLGVGFVPVRKLGKLPAETVTAAYELEYGANT
                     VEMHSDAIENGERVIVVDDLLATGGTTRATIELVERLGGDVVALAFLIELTELGARQY
                     LEDYNIITLIRF"
     gene            complement(2581..>4211)
                     /locus_tag="VFH60_11215"
     CDS             complement(2581..>4211)
                     /locus_tag="VFH60_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314397.1"
                     /translation="MKNYLDVLSSERRNLRTVVYLLLAAMTFGLGLTFASTAQSAPQQ
                     QTEVKISGTVTLGSAGAEGVTVELRQHANSGAESLLATTTTNGAGHYAFANQPSAPGD
                     AFYYIEFKGGAGTLANWRTFPIIYLNGTDFTVPTVDLSDVVLVEPAAGATVGPGSKLK
                     WNARRAGETYRVYIYAAGQGDKPVVDSGKLGGSAEYTLGDGGLAEGQYEAIVQVRDAV
                     IGYGNSRARFQFTFGKVVVALPTPQQDQGGAASAQPTSEKQITLPTAQPTSPEGGEAA
                     PADNNQHAQPTAATPAQVQPTAAAEPATKPDVKLRLSADKTSVNQGQSLVYVIEVHNA
                     GDAAATDVVVTDKLPAGVAVDAAQMRSTHGSVAVTDNTVTAHVGELVPNGTAQVQIPV
                     LVGPEAGSALSNQASAVYKQSSEAVQSNAYIAQVAEPLTAAQPAPTEEAQPTATQAAQ
                     SPVQEPQPTATTPSRPESTPVVASDEGTSGSESASGQAPATTPNTPTPRPTQARAAAA
                     IPATGGSFPLVLALLFLVMALLARYLRGSRTRKL"
BASE COUNT          736 a         1360 c         1287 g          828 t
ORIGIN      
        1 atcacggtgc gcttctgtgg cgatcacaat agcggcatcc ccggcctcaa gagccgaacc
       61 gacgaaccgg cccaacgcat ccaacaagaa atggtcgtca tcgtagaact ggacggcatg
      121 gccacaggaa tcgtagtccc ccagtgtaac tgggagcacg ttcgatgtgg ttggatagcc
      181 cgtactgctg ctcatacctg ccctgactct cctgctggtt cggtcgtggc tgctgctcgt
      241 gcatttaccg cccaaccgta cgccatgccg atttcccgcc gctcgaataa gcaagccgct
      301 tcattgcatc tgcaatatac atgccgcgtt accctcacca gtcctaatac tatacgactt
      361 ttatctacct tttagcaagg tccggcgcag tggcataggt catttgttca atactgcctg
      421 actttggggc tgcctgtgtg gcatcattca ccaaagatat acacatctag gaaggcattt
      481 cggaatttac cctgtggatc gtaggtacgt agcagtcgtt gaaagtccgg caacttcggg
      541 taaagcgatt ggactctttc gggggatgta gcaaacagct tgccccagtg agggcgagcg
      601 tccagcggcg cgagttgttc ttccaccagt ggaagcacca gactgaacgc ctcccaatct
      661 ttctcccagg taaagtgaat cgctacacag tcctgcccgt agcagggact cagccacaga
      721 tcgtcggcgg cgacggtgcg cacctcggag atctgcaaca gcgggaccac gtgctcccgc
      781 aggctgtaga tggcgcggaa ggccgccatg ccgtgccggc ggggcagcag gtactcggtt
      841 tgcagctcct cgccgctact cggcgtgaag cccatcttga agtgcggcag gcgtgcgtac
      901 caggccccgc gaatgcccat ctgctcggtg caaggttccg ctgagaagcc ggggatgggg
      961 tgccggtggg ttggcgcggg tgcggccccg aagaactccg gcggcaattc aaacgtcgcg
     1021 ccgtccgtaa cgggccgctt caaccagacc tggttcaccc gcgcatctcg ccaattcaac
     1081 aagaggctga cgctgtatgc gctcgacgtg atctcatcga aatgggcttc caactgcgcg
     1141 aaggggaggt cctcgtacac gtcctggcgc atatcgaaag ccgggacaac atcgaggcta
     1201 agcttcgtaa ccacccccag accgcccagc ccaaccaccg ccccccggaa ttgctcgcct
     1261 cctgcgccac gttcgtcgcg agaaagcacg accaccgtac cgtcggctgt gacgagttcc
     1321 atcgccgcca cggccgtggc caggttgcca ttgcggtcgc ctgagccgtg ggtggccgtc
     1381 gcacaggcac ccgcgacgga aatgtgcggc aatgaggcca tgttgcgcag cgcgtagccc
     1441 tcacggtgaa gctcctcgca gaaccggccg taccgggcac cggcatcgat tgtcacggtg
     1501 ttgcgctcgc ggtccagcac gaatacacgc tcgagacggt cgagtaagat gagatcgccg
     1561 ggggagtccg cgacgtgatt gaacgaatgg cgagaaccga gcgccttcaa tttgctcgcg
     1621 ccactcacca gttcctggac ttgctccacc gtttcgggaa agtgcaacat ggcggcacga
     1681 taggtgtaat tgcccgccca attggttgga ttgccaatca tccttgcgcc cctacccatc
     1741 acccctgtgc tcgcgaggag cggaccgtgt gcctggcatt acgggccgtt tcccgcaagc
     1801 gcgaggtgcg cggacaaaag ggaggtcgta ctcagacctc cctcccgctt tgaccctgac
     1861 agggcatgcc ggcgtctatc tttaccaatc gccttttaga gtcggcccgc cctgttcggc
     1921 agttgcaccg gggccatcaa aagcgtatca gggtgatgat gttgtagtct tccaggtact
     1981 ggcgcgcccc gagttctgtt agctcgatga ggaaagccag ggccacaacg tcgccgccga
     2041 gccgctccac aagctcaatc gtggcgcggg tggtgccccc tgtcgccagc aggtcgtcca
     2101 ctacaatcac gcgctcccca ttctcgattg cgtcgctgtg catttccacc gtgttcgcgc
     2161 cgtactcaag ctcgtacgca gccgtcaccg tctcggcggg cagcttgccc agcttgcgca
     2221 ccggcacaaa gcccaccccc agcctgtagg caatcggcgc accgaagatg aagccacggc
     2281 tttcaatcgc cactaccttt gtggcgttga gaggggccag cttgtcgcag aactggtcta
     2341 ccacctgctg gaacgccggg cctaccttga gcaaggtagt aatgtcgcgg aacaggatgc
     2401 cctgcaccgg aaagttgggt atatcgcgga tcaattcggt cagatcgtag tccattagcc
     2461 ccccaggcga gtcgccaatc tctaagccgt tcgagcacac gggagcgtat cgccccgcgc
     2521 tattatatca ctccagggtg caagattcgc gcctgcatct cgcctacatc tagccaatat
     2581 ctacagcttt ctggtcctgc tgccccgcag gtacctcgcg agcagcgcca tcaccaggaa
     2641 cagcaaggca agcaccagcg ggaacgagcc gccggtcgca ggaatagcgg ccgcagccct
     2701 ggcctgggtg ggccggggag tgggcgtatt aggggtcgtg gcgggtgcct ggccggaagc
     2761 gctctcactc cccgacgtgc cttcgtcgga cgcgacgacc ggggtactct caggtcgtga
     2821 cggggtagtg gcagtcggct gcggctcctg caccgggctt tgcgctgcct gcgtggccgt
     2881 gggttgcgcc tcctcggtgg gggcgggctg ggcggcggtc agtggctcgg ctacctgcgc
     2941 gatataagcg ttcgactgca ccgcctcgct gctctgcttg tacaccgcgc ttgcctggtt
     3001 gctgagcgcg ctacccgctt ccggccccac cagcacgggg atttgcacct gcgccgtgcc
     3061 gttggggacc aactcaccaa cgtgcgccgt caccgtgttg tccgtcaccg ccaccgagcc
     3121 gtgggtgctg cgcatctgtg ccgcgtccac cgcgacaccg gccgggagct tgtcggtcac
     3181 taccacgtcg gtagcggcgg catcacccgc gttgtgcacc tcgattacgt agaccagcga
     3241 ctgcccctgg ttaaccgagg tcttatcggc ggagaggcgc aacttgacgt ccggctttgt
     3301 agcaggttcc gccgctgctg taggctggac ctgggccggg gttgcggcag tgggttgtgc
     3361 gtgctggttg ttgtccgccg gtgccgcttc gcctccctcc ggcgacgtgg gctgggcggt
     3421 aggcagggtt atttgctttt cgctggtggg ttgcgcggaa gccgcaccgc cctggtcctg
     3481 ctggggcgtg ggtagcgcga ccacaacctt gccgaacgtg aattggaagc gcgcccggct
     3541 gttgccgtag ccaatcaccg cgtcgcgcac ctgcacgatg gcttcgtact gcccttcggc
     3601 aaggccgccg tcgcccaggg tgtactcagc gctgccgccg agcttaccgc tgtccacaac
     3661 cggcttgtca ccctgccccg ccgcgtaaat atacacccgg tacgtctcgc ccgcgcgccg
     3721 cgcgttccac ttgagcttgc tgcctgggcc gacggtcgcc ccggcggcag gctccaccag
     3781 tacgacgtcg gagagatcga ctgtcggtac ggtaaaatcg gtgccgttga gatagatgat
     3841 cgggaaggtg cgccagttcg cgagcgtgcc cgcgccgccc ttgaactcga tgtagtagaa
     3901 ggcatcgccg ggggcgctcg gctggttggc gaacgcgtag tgccccgcgc cgttggtggt
     3961 ggtggtggcg agcaggctct cggccccgct gttggcgtgc tggcgcaact ccaccgtgac
     4021 accctcggcc cccgcgcttc ccagcgtcac ggtgccgcta atcttgactt ccgtctgctg
     4081 ttggggcgca ctctgggcgg tggatgcaaa agtcagcccc agcccaaagg tcatagcggc
     4141 gagcagcagg tatacaacgg tcctcaggtt tctccgctct gaactcagca catctaagta
     4201 gttcttcatc t
//
LOCUS       DASRWZ010000711         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1223068, whole genome shotgun sequence.
ACCESSION   DASRWZ010000711 DASRWZ010000000
VERSION     DASRWZ010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223068"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /gene="uvrC"
                     /locus_tag="VFH60_11220"
     CDS             complement(<1..1316)
                     /gene="uvrC"
                     /locus_tag="VFH60_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET6314398.1"
                     /translation="MAISPSIPPRERAPGFEERLRALPGTPGVYIMHNRADDIIYVGK
                     AVSLKNRVRSYFGSLKGQAPKVMRMVDEVFDFEYILTDTELEALILENQLIKKHRPRY
                     NIKLKDDKTYPYIKVTTNEAWPRVLPTRRVLNDGAQYFGPFPGMGTVYQTLDLLDKLF
                     PFRTCDKEITGNDARPCVQYFIHRCLGPCAGLADKPAYDDAIKQVVLFLNRKHDIIIR
                     NLKSKMGEASENLEFERAAVFRDRIRSLERVLEEQKVFSATKTDEDVIAFARNDGEAC
                     VQIFFIRGGKLLGREHYMLEGTADSTPGEILESFMTQFYDDASYVPPKVVLQDDVSDA
                     EIIESWLRVRRGTKVTLSVPRRGQKRDMVEMAARNAAETLEQFRLRWLSDEQKSTAAL
                     TELQALLRLPVWPQRIECYDVAHLQGTDVAGAMVVFEQGMPKKKEYR"
     gene            complement(1371..>2997)
                     /locus_tag="VFH60_11225"
     CDS             complement(1371..>2997)
                     /locus_tag="VFH60_11225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6314399.1"
                     /translation="WWFRTALLEQQRSPLSDPGSYAPFGFNLGQGEPTLANTLPGVVL
                     GALTNDVVAYNLLALGSFVVSGIGAYLLVKELSSSRLAALVSGLAFAFCPYRLSQFAG
                     HLQLLGTGWIALAFYFLERLLRTGRVGDGLLLGLSFGLAALSAWYYAYMVGLALGAYL
                     LLRLALGRRGYNVRKVLVGLLAGAVLFLALASPVAIPSLQLWAEGGLTHSAKAADEHS
                     AAPADYVVRNPLHPLWGEVGMRAHAEQNVIESALYVGIVLSFVTVGGWVTLRRRTAVS
                     ERRVWSTWLALGALAFILSLGLSLHDANGQVRVGAGGVVPLPGQLLYDWLPLYSSMRA
                     FARFGVIVALALAVLLGLVWSAGLRQGPSKVRRNPHFATFVLAFLLMIDFWTAPYAWG
                     TTRVEASQTARFLSQQPPGTVMQMPLHASQSGPALYRRVTSGKPVAYGYETFEPPAWR
                     ARRDELQEFPEGASLDVLREWGVRYVVVSGKPYGADWAGLLDHLKTLPGLRYMAAFED
                     RSPWTVDPQVLDARPDMVEYAMPENNAVFELVR"
BASE COUNT          567 a          994 c          916 g          520 t
ORIGIN      
        1 cggtactctt tcttcttcgg catcccctgc tcgaacacca ccatcgcgcc cgccacgtcc
       61 gtgccctgca agtgcgccac gtcgtagcac tcgatacgtt gcggccagac cggcagccgt
      121 agcaacgcct gtagctcggt gagggccgcg gttgacttct gctcgtcgct cagccagcgc
      181 aggcggaact gctcaagcgt ttcggcggca ttgcgggcgg ccatttcgac catgtcgcgc
      241 ttctgccctc ggcggggcac cgacagcgtg actttggtgc cccgcctcac gcgcagccac
      301 gactcgatta tctccgcgtc ggaaacgtcg tcctggagca caaccttggg gggcacataa
      361 gaagcatcat cgtagaactg cgtcatgaag ctctcgagga tttcgccggg cgtcgagtcg
      421 gccgtgccct cgagcatgta atgctcccgc cccagcagct tgccgccccg tatgaaaaat
      481 atctgcacgc aagcttcgcc gtcgttgcgg gcgaacgcga tcacgtcctc gtcggtcttg
      541 gtggcgctaa agaccttttg ctcttcgagc acgcgttcga ggctcctgat gcggtcgcga
      601 aacacggccg cgcgctcgaa ctccaggttt tccgatgcct cgcccatctt gctcttcaag
      661 ttgcgaatga taatatcgtg cttgcggttg aggaagagca ccacctgctt gatagcgtcg
      721 tcgtaggcag gcttgtcggc cagccccgcg catggtccca ggcagcggtg gatgaagtac
      781 tgcacgcaag gccgggcgtc gttgcccgta atttccttgt cgcaggtgcg gaaggggaac
      841 agcttgtcca gcaggtcgag cgtctggtag accgtgccca tgccggggaa cgggccgaag
      901 tactgcgccc cgtcgttcag cactctcctg gtggggagca cgcgcggcca cgcttcgttg
      961 gtggtcactt tgatgtaggg gtaggtcttg tcatccttta gcttgatgtt gtagcgcggg
     1021 cggtgcttct tgataagctg gttttcgagt atcagcgcct ccagctcggt gtccgtgagg
     1081 atgtattcga agtcgaacac ctcgtccacc atgcgcatga ccttgggggc ctgccccttg
     1141 agggagccga agtaggagcg cacgcggttc ttgagcgaca ccgccttgcc gacgtagatg
     1201 atgtcgtcgg cgcggttgtg cattatgtaa acgccgggcg tgccgggcag cgccctgagc
     1261 cgctcctcga agccgggggc ccgctcgcga ggtgggattg acggagaaat cgccatacct
     1321 gaatattacc agaaagtgcg cgtttgttct attgtgtaac ctcagctgcc tcagcgcaca
     1381 agctcgaaca cagcattgtt ttccggcatc gcgtactcaa ccatgtccgg ccgcgcgtca
     1441 agcacctgcg ggtctacggt ccaggggctg cggtcctcga aagccgccat gtagcgcaga
     1501 ccggggagcg tcttcaggtg atcgagcagg cccgcccagt ccgccccgta tggcttgccg
     1561 ctcaccacaa cgtaccgcac gccccactca cgcagcacat ccagcgacgc cccttccggg
     1621 aactcctgca attcgtctcg ccgggcgcgc caggccggag gctcaaaggt ctcatagccg
     1681 taagcgacgg gcttgccgga ggtcacccgg cggtacaagg caggccccga ttgagaggcg
     1741 tgaagcggca tctgcatcac agtgccgggc ggctgctgcg acaggaagcg ggcggtctgg
     1801 ctggcctcca cgcgagtggt accccaggcg tatggcgcgg tccagaagtc tatcatcagc
     1861 aggaaggcca gcacgaacgt agcaaagtga gggttacggc gcactttgga cgggccttgc
     1921 cgcagccccg cgctccacac aagccccagc aataccgcaa gcgccagggc cacaatcacc
     1981 ccgaacctcg caaacgccct catcgaggag tagaggggca gccagtcgta gagcaactgc
     2041 cccggcagcg ggaccacgcc gcccgcccct actctcacct ggccgttcgc gtcgtgcaaa
     2101 ctcaggccta gcgataagat gaaagccaac gcgcccaggg caagccaggt gctccaaaca
     2161 cgccgctccg agacggcagt acgccgccgt aacgtcaccc agcctcccac ggttacaaac
     2221 gaaagcacga tacccacgta cagggcgctc tcgattacgt tctgctcggc gtgcgcccgc
     2281 atgcctacct ctccccacag cgggtggagg gggtttcgca ccacgtagtc cgccggtgcc
     2341 gcagagtgct cgtcggccgc cttggcgctg tgcgtcaacc caccttccgc ccacagctgg
     2401 agcgacggta ttgccaccgg acttgccagc gcgagaaaga ggaccgcgcc cgcgagcagc
     2461 cccaccagga ccttgcgcac gttgtacccg cggcgaccga gtgccaggcg caggagcagg
     2521 tatgccccca gggcaagccc caccatgtag gcgtagtacc aggccgaaag cgccgcgagc
     2581 ccgaacgaca gcccgagcaa gaggccgtca cccacccggc ccgttctgag caggcgttcc
     2641 aggaagtaga acgccagcgc tatccagccg gtgcccaaca gttgcaagtg cccggcgaac
     2701 tgcgacagcc ggtaggggca gaatgcgaac gccagccccg aaacgagggc ggccagtctg
     2761 ctggatgaga gttccttcac cagcaaatac gctccgattc cggataccac gaacgagccg
     2821 agggccagca aattgtacgc cactacgtca ttcgtcagcg cccccagcac cacgcccggc
     2881 agggtgttgg ccagcgtagg ctccccttgc cccaggttga agccaaacgg cgcgtagctg
     2941 ccaggatcgg agagaggcga gcgttgttgt tccaataagg ccgtgcggaa ccaccac
//
LOCUS       DASRWZ010000712         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_195200, whole genome shotgun sequence.
ACCESSION   DASRWZ010000712 DASRWZ010000000
VERSION     DASRWZ010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195200"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..794)
                     /locus_tag="VFH60_11230"
     CDS             complement(81..794)
                     /locus_tag="VFH60_11230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6314400.1"
                     /translation="MYQRNARYYDALYSWKDYAAESTKLVELVQAQAQRPAGTLLDVA
                     CGTGAHLVYLRDYFHIEGVDFEPEMLSIARDRLPGVALTQGDMVDFDLGRSFDVVTCL
                     FGSIGYVKTLERLNQALANMARHVSPGGLLVIEPWFMPERFQPGTIHSLFVDQPDLRI
                     ARMNVSTIEGRLSILHFHYMVGTPQGIDYWDERHEMALFTHEEYTQAFHSAGLDVSYD
                     EQGLWGRGLYTGLKSEAAE"
     gene            1306..2667
                     /locus_tag="VFH60_11235"
     CDS             1306..2667
                     /locus_tag="VFH60_11235"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HET6314401.1"
                     /translation="MVRLQKRGSVRLGVGIVLAGLAVVAVLLLRAMYAPETVPVPQDT
                     ALLPALAEATVVVPGAPQCWPCPTPAGAEAQATPTIAIPTPEPTASPTPGPVFGLAWF
                     HKPPEDGTTPEQMTQAHRYIHLTSLADIEYRDQLRDAGYDGPIYTYITANAVEGPGPY
                     ANSSEECEAGYEPFDNTLTWKTDDFCRYVHAHESWFLHNGEGERLVDDYFGSGRWTYL
                     MNPADPGWQAFAQERLVVMRDEWGYDGVWLDNVDLDLARAKFDVENGDGRVREFAGDV
                     EWRAGIESWLRGVRSAVGEWPIWANLVGGGKAADSWDPYAPYLDGAMDESFGVRWVDE
                     WRPAQDWHGQLERASRWLGAGKGLVMVGQGAKEDHERLLFTLASYLLVAEGEQAFYRY
                     TRFDSYYNSVWLYPEFDTARQLGRPTGPRDEVVPGVWRRTFTNGTVEVDLNTREGKMD
                     VRP"
     gene            2733..>3524
                     /locus_tag="VFH60_11240"
     CDS             2733..>3524
                     /locus_tag="VFH60_11240"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HET6314402.1"
                     /translation="MGNRRKLAVLFCLGLALLGLGFPGVGIAAKGDGKTEVGGSALDA
                     RSANSRIAAENALPGTDEWADVGHHDMSKLSAFTGAVSVNAGEALNVHVKSTGSSLSA
                     KLYRLGYYQDHGARFYWERTNIATGSQPACTRDKDTGLVQCPWSPTFAVPTDPAWISG
                     IYLLRLDSNQGYRFFVYFIVRNDGYPADILVMEPTKTNQAYHRYGCESLYFSGTATAS
                     NNQCDGVVSNGNSNRTRGHKVSFDRPYKGGAGTGGLFTHDVEMVRW"
BASE COUNT          660 a         1017 c         1175 g          672 t
ORIGIN      
        1 tgagatcctt cgctacgctc aggatgacag aggggtgcct ggcccgactg gaccggcggg
       61 gctattttca cgttccacct ttattcagcc gcctcacttt tgagacctgt gtacagccct
      121 cggccccaga gaccttgttc gtcgtaactt acatctagac cggctgagtg gaaggcctgg
      181 gtgtactcct cgtgagtgaa gagggccatt tcgtgccgtt cgtcccagta gtcgatgcct
      241 tgcggggtgc cgaccatgta gtggaagtgg aggatagaga ggcggccttc gattgtgctg
      301 acgttcatgc gggctatgcg caggtcaggc tggtccacga agagcgagtg aatggtgccc
      361 ggctggaagc gttccggcat gaaccagggt tcgattacga gcaagccacc ggggcttacg
      421 tgccgggcca tgttcgccag cgcctggttg aggcgttcga gagtctttac gtagccgatg
      481 gagccaaata gacacgtcac cacgtcgaag gagcggccca ggtcgaagtc gaccatgtcc
      541 ccctgcgtga gggctacgcc aggcagtcgg tcgcgggcta tgctcagcat ctcaggctca
      601 aagtctacgc cttcgatgtg gaaatagtcg cgcaggtaga caaggtgcgc gccggtgcca
      661 caggctacgt ccaggagggt gccggcggga cgttgtgcct gggcctggac aagctctaca
      721 agcttcgtgc tctcggcggc gtagtccttc caggaatata gggcatcgta gtacctggcg
      781 tttctctggt acattcttgt ctcctcttgg aacttactct gggcaggtct caaatttctg
      841 ggaagtgtta gccattgact cgtatgagtg gcagcacgtc tggcctggcg gtttgctagg
      901 ccgggcgggg tactggtaca ctgattgcta catgctcctg agacgcgttc gaaggcgcgt
      961 cactgcgccg gtgcgtgatt atacatacat gaccggcggg tgcgtcaagc gggcgcgagc
     1021 gtatccgacc tccaaccacg gtagcatgtt gtccgcggat gccccctctc ccagacttat
     1081 agctactctt gcgttgcacc ccttctaact ctaatgggtg atatagacgc accctctgct
     1141 gccgtacaat catggggtac gagcatgccc acttcgccgt ctctaacctc gcgggaatat
     1201 aagatagctg gaaggcgttc cagctagagg atgcaggggc attgcacggg tccgtgagga
     1261 aagaaacagg ggccattttt gatagggaag aggttcagag cactggtggt aagacttcaa
     1321 aagcgaggtt cggtacggct gggcgtgggc atcgtgctgg cggggctggc ggttgtggct
     1381 gtgttgctgc tgcgcgccat gtacgcgcct gagacggtgc cggtgccgca agatacggcc
     1441 ttgctgccgg cactggccga ggcgacggta gtagtaccgg gcgcgccgca gtgctggccc
     1501 tgccccacgc ctgccggagc cgaggcgcag gcaaccccca ccattgcgat accgacgccc
     1561 gaacctaccg catctcccac gcccggtccg gtgtttggcc tggcctggtt ccacaagccc
     1621 ccagaagacg gtaccacccc ggagcagatg acacaggccc atcgctatat ccacctgacc
     1681 agcctggccg atatcgagta ccgcgaccaa ctgcgcgacg cgggctacga tggtcctatc
     1741 tatacctata tcaccgccaa tgcggtcgaa gggccagggc cgtatgccaa ttcctctgaa
     1801 gagtgcgagg cgggctacga gccgtttgat aacaccctga cctggaagac ggacgatttt
     1861 tgccggtacg tacacgcgca cgaatcgtgg ttcctgcaca acggcgaggg cgagcggctg
     1921 gtggacgact atttcggctc agggcgctgg acgtacttga tgaaccccgc cgacccgggt
     1981 tggcaagcct tcgcccagga acggctggtc gttatgcgcg acgagtgggg ttacgacggg
     2041 gtatggctcg acaacgtgga cctcgacctg gcgagggcga agttcgacgt agagaacggc
     2101 gatggacggg taagggagtt cgccggtgac gtcgagtggc gggcgggcat cgagagctgg
     2161 ctgcgaggag tgcgctcggc ggtgggtgag tggccaatct gggcgaacct tgtaggcggc
     2221 gggaaagccg ccgattcgtg ggacccctac gcgccttacc tggatggagc gatggacgag
     2281 tcgttcgggg tgcgctgggt agacgagtgg cgcccggcgc aagattggca cggacagctt
     2341 gagcgggcga gccgttggct gggcgcgggc aaggggctgg tgatggtcgg ccagggcgcg
     2401 aaagaggacc atgagcggct gcttttcact ctcgccagct atctgctggt ggccgagggc
     2461 gagcaggcat tctaccgcta cacccgcttc gactcgtatt acaactcggt gtggctctac
     2521 ccggagttcg acaccgcccg ccagcttggc aggcccaccg gcccccgcga tgaggtagtg
     2581 cccggagtgt ggcggcgcac cttcaccaac ggcacggtcg aagtggacct caatacgcga
     2641 gaaggcaaga tggacgtgag gccgtaactc gtaattcgta actcgtaatt cgtaactcgc
     2701 ggagggtaag gttgggcgat agaggcgcta gagtgggcaa tcgtaggaag ctggcggtgt
     2761 tgttctgcct ggggttggct ttgcttgggc tggggtttcc tggggtgggt atagcggcga
     2821 aaggtgatgg caaaacggag gtgggaggca gcgccctgga tgcgaggtcc gccaatagcc
     2881 gcatcgcagc cgagaacgcc ctgccgggta ccgacgagtg ggccgacgtg ggccaccacg
     2941 acatgagcaa gctgtcggcg ttcactggag cggtgagcgt caacgcgggc gaggcgctca
     3001 acgtacacgt gaagtccacc ggctcgtcac tgtccgcgaa gctgtaccgg ctcggatatt
     3061 accaggacca cggggcgcgt ttctactggg agcgcaccaa catagccacc gggtcgcagc
     3121 ccgcctgcac ccgcgacaag gacaccgggc tggtgcagtg cccctggtct cctacgttcg
     3181 ccgttcccac cgaccccgcc tggatcagcg gcatctacct gctgcgcctc gactcgaacc
     3241 agggctaccg cttcttcgtc tatttcatcg tccgcaacga cggctacccc gccgacattc
     3301 tcgttatgga gccgaccaag accaaccagg cttaccaccg ctacggctgc gaaagcctct
     3361 acttctccgg tacggccact gccagcaaca accagtgcga cggggtggtc tcgaacggca
     3421 acagcaacag gacaaggggc cacaaggtgt cgttcgaccg cccgtacaag ggcggcgcgg
     3481 gcacgggcgg gttgttcacg cacgacgtgg agatggtgcg ctgg
//
LOCUS       DASRWZ010000713         3054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1223182, whole genome shotgun sequence.
ACCESSION   DASRWZ010000713 DASRWZ010000000
VERSION     DASRWZ010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223182"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1158
                     /locus_tag="VFH60_11245"
     CDS             55..1158
                     /locus_tag="VFH60_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314403.1"
                     /translation="MPTTDAPGAETPIASSAVTLRNLPTPRVVKCPTPGCPGWITLDI
                     TRHQTEPCRYCRIGVDTLRFLLSRIGAEHKALPPEVHEEFNRLMLTMAGAWGQNVYLR
                     PSPTTLVDLKAGVHGVVVEYNPLLGNAGADGPAPLVGIILHKLLHFEAHLGQRGPQIG
                     IKSGSRDKEGMVPLMNYLSTVTDHAWVTARLEQLNPALRQAQSRWGLDAAQMLVGSES
                     MFNRYLSERNLQRIVSVLKETSDPEQFRKSVLSRLATLTAQAVEREKEDTRRMYLALQ
                     LANVRLMNAESYAEYQQALEAAGLQNVRESYPLAERLSNDLANSPAAHPPAQTSLSND
                     AGHAVVLESAQFHRALEGAIKSLGMANYFEVKS"
     gene            1172..1399
                     /locus_tag="VFH60_11250"
     CDS             1172..1399
                     /locus_tag="VFH60_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HET6314404.1"
                     /translation="MQCDICGKEGAHVRRVARTYGKGANLLVIENVPVVSCPHCGESY
                     LTADTLHEIERIKLHRRSFAVTRPVEVASFA"
     gene            1418..>3054
                     /locus_tag="VFH60_11255"
     CDS             1418..>3054
                     /locus_tag="VFH60_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314405.1"
                     /translation="MIKWLSKVFDSTDKELQRLQRTVGQANEEIMQLLESQRDQIGRM
                     REDALEVVCSANVGERAGSIYSSEMRKVAADARRLSEQVGSLRETIERGEQTSRELSV
                     LLADARTLSSMNAEKSQRPPAERRELEEEIEALVAELSEQLAGVMEAKPDLREAILRD
                     VPKARFHPHKPGPEEMIKQVDATASFLGREVADYFSRINETLLALRRGVALSLLAGIV
                     PQGSCFRPSSPEDLDGAVRGLVTGLVQNDLIRHLQRQGLRLPSEIKNAAGLHEGQAPA
                     PQEGRGRRGRDNAPAQPEQGKDDLADELYWEEQATAELLAIACEVVTEGEPAQNINNI
                     MSRFQAREERLQARAAAFRGRILEARTDYWLGIQARIADIVGAEAMSPFIERFEELDA
                     EQDELLEQVTRQDEDPDLPVMIKPEQVGEFMRRRDSLEEATFTELMNELRPTNRGAHD
                     SLKALSDEFRKEQRKDEQRVLSDLIPEVFACVWEASRRTNGMRPYDVQLMGGIVLHEG
                     KISEMKTGEGKTLVAVAPLALNGLTARGAHLCTVNEYL"
BASE COUNT          618 a          996 c          979 g          461 t
ORIGIN      
        1 agggtgcggt accacgccgc tatatgcgac cattgattgg agccagtaat ttccatgccc
       61 actactgacg cgccgggtgc cgagacccct atcgcctcct ccgctgtcac cctgcgcaac
      121 ctgcccacgc cacgagtggt aaagtgcccc acgccgggct gtccgggctg gattaccctt
      181 gacataactc ggcaccagac cgagccgtgc cgctactgcc gcatcggggt cgataccctg
      241 cgcttcctgc tgtccaggat cggggccgag cacaaggcgc tgccaccgga ggtccacgag
      301 gagttcaatc gcctgatgct cacgatggcg ggcgcctggg gccagaacgt ctacctgcgc
      361 ccttcgccca ccaccctggt ggacctcaag gcgggggtgc acggcgtggt ggtcgagtac
      421 aacccgctgc tcggcaacgc gggcgcggac ggcccggccc cgctcgtggg catcatcctg
      481 cacaagctac tgcatttcga ggcccatctg ggccagcgcg ggccgcagat aggcatcaag
      541 tcaggctctc gcgacaaaga gggcatggtg cccctcatga actacctctc gaccgtcacg
      601 gaccacgcct gggtgacggc ccgccttgag caactcaacc ccgcgctccg ccaggcgcag
      661 agccgctggg gcctggacgc cgcgcagatg ctggtaggct cagagagcat gttcaaccgc
      721 tacctatcgg aacgcaacct gcagcgcatc gtatccgtac ttaaggaaac gtcggaccct
      781 gaacagttcc gcaaatcggt gctgtcgcgg cttgccaccc tcaccgcgca ggcagtcgag
      841 cgcgagaagg aagacacccg ccgcatgtac ctggctttgc agctagccaa cgtgcgactg
      901 atgaacgccg agtcctacgc cgagtaccag caggcgctgg aagccgccgg actgcagaac
      961 gtgcgcgaat cgtaccccct ggccgagcgc ctctccaacg acctggcaaa ttcgcccgcc
     1021 gcccacccgc ccgcgcagac aagcctcagc aacgacgccg ggcacgcggt ggtgctggaa
     1081 tcggcgcagt tccacagggc gttagaaggt gccatcaagt cgctgggcat ggcgaactac
     1141 tttgaagtga aaagctaggg ctgggaacca gatgcaatgt gatatttgtg gtaaggaggg
     1201 cgcgcatgta cgccgggtcg cccgaactta tggcaagggt gctaacctgc ttgttatcga
     1261 aaacgtacct gtagtaagtt gcccgcactg tggtgagagc tatctgaccg ccgatacgct
     1321 ccatgagatc gaacgcataa agctccacag gaggagtttc gcggtcacgc gccctgtgga
     1381 agtggcaagt ttcgcctgag atgaaggaca caaaatcatg ataaagtggc tcagcaaagt
     1441 attcgatagc acagacaaag agcttcagcg gctgcaacgc actgtggggc aggccaacga
     1501 ggagatcatg caactgctag aaagccagcg cgaccagatt ggccgcatgc gcgaagatgc
     1561 cctggaggtc gtgtgctcgg ccaacgtggg cgagcgcgcg ggcagcatct acagcagcga
     1621 gatgcggaaa gtggccgccg acgcccgcag gttgtcggag caggtgggca gcctgcgcga
     1681 gacgatagag cggggcgagc agaccagccg cgagctttcg gtgctgctgg ccgacgcgcg
     1741 taccctctcc tccatgaacg ccgagaagtc gcagcgcccg cccgccgagc gccgcgagct
     1801 tgaggaagag atagaagcgc tggtggccga gctatcggag cagcttgcgg gcgtcatgga
     1861 ggccaagccg gacctgcgcg aggccatcct gcgcgacgtg cccaaggcca ggttccaccc
     1921 gcacaagccc ggccccgaag agatgatcaa gcaggtagac gctacagctt cgttcctggg
     1981 ccgggaggta gccgactact tcagccgcat caacgagacg ctgctggccc tgcgccgtgg
     2041 ggtcgccctg agcctgctcg cgggcatagt gccgcagggt tcgtgcttcc gcccctcgtc
     2101 tcctgaggac ctggatggcg cggtgcgcgg cctggtcacc gggctggtgc agaacgacct
     2161 tatcaggcac ctgcaacggc agggcctgcg cttgccttcc gagatcaaga acgctgcggg
     2221 gctgcacgaa ggccaagcac ccgcgccgca agaaggtcgt ggccgccgcg gccgcgataa
     2281 cgcccccgct cagcccgaac agggaaagga cgacctggcc gacgagcttt actgggaaga
     2341 acaggccacc gcggaattgc tggctatcgc ctgcgaggtt gtcaccgaag gcgaaccggc
     2401 gcagaacatc aacaacatca tgtcccgctt ccaggcccgt gaggagcgct tgcaggcccg
     2461 tgccgccgcc ttccgcggcc gcatcctgga ggcccgcacc gactactggc tcggcataca
     2521 ggcccgcata gccgacatcg tgggtgccga ggccatgtcg cccttcatcg agcggttcga
     2581 ggagcttgac gccgagcagg acgaactgct cgaacaggtg acccgccagg acgaggaccc
     2641 agacctgccc gtgatgataa agccggagca ggtgggcgag ttcatgcggc ggcgcgacag
     2701 cctggaggaa gccaccttca ccgagctgat gaacgagcta cggcccacca accggggcgc
     2761 ccacgacagc ctgaaggccc tgagcgacga gttccgcaag gagcagcgaa aggacgagca
     2821 acgggtcctc agcgacctca tccctgaggt gttcgcctgc gtatgggagg cctcgcgccg
     2881 caccaacggc atgcggccct acgatgtgca gctcatgggc ggtatcgtgc tccacgaggg
     2941 caaaatatcc gagatgaaga cgggtgaagg caagaccctg gtggccgtgg cccccctcgc
     3001 cctcaacggc ctgaccgcgc gcggcgcgca cctgtgcacc gtgaacgagt acct
//
LOCUS       DASRWZ010000714         4649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_401649, whole genome shotgun sequence.
ACCESSION   DASRWZ010000714 DASRWZ010000000
VERSION     DASRWZ010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401649"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1326
                     /locus_tag="VFH60_11260"
     CDS             70..1326
                     /locus_tag="VFH60_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6314406.1"
                     /translation="MSDQQSTQSLHSAEDLGSPTPTEPGADGSLEGSNGSGGGSPTVA
                     MGGGTPTVSTATGTTEIPKPPNGARPDVLLEVDRLVKYFPVRGGLLQRTVAWTKAVDD
                     VSFFIRQGETLGLVGESGCGKTTVGRTLLRLTPATGGEVRFQGQNIFDLKADHLKRVR
                     RDMQIIFQDPFSSLDPRVPIGESIGEGLIIHGVSKNERQDRVYDALAHVGLLPEHAKR
                     YPHEFSGGQRQRIGIARALVLRPKFIVCDEPVSALDVSIQSQVLNILKDLQGEMGLTY
                     LFIAHNLSVVEHISDRVGVMYLGKMAELAERTALFASPQHPYTHALLSAIPIPDPKIR
                     RERIILKGDVPSPLNPPTGCRFHTRCWLAQDICKQQVPEWRNVAPPGQLEHFVACHFA
                     PFTKMPLPGETSSIIGKDETTVVQHQ"
     gene            complement(1438..2745)
                     /locus_tag="VFH60_11265"
     CDS             complement(1438..2745)
                     /locus_tag="VFH60_11265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6314407.1"
                     /translation="MLSRLSHAIRSLWGWTLSRPGLVLGALVLVGFALRVGWLVASGG
                     LFAPPQEDELDYNELAKSLLSGRGYSLDGQPQVNRSPGYPVLLFLVYSVFGVQPAVAR
                     LVQVALLVAVVPLLYYVGSRGWSRSVGMLAAHVFAFHPFSIFWSRYLATENLLVFVYA
                     VLAALLVCPERAGAWRLLAGGGVLGVALLTRPTAILVAVGLVLWLLLVSRGRGRLVRP
                     ALLLAGALVALSPWVARNFAEYGQFIPLTSGYGSSAGGYVFWISNNEFTARPGERWGR
                     YVPAEMLPDYAEYISHTGNPGLLDRKGYEYGLRYLTSHPQDVPVLLLGTFLRFWNVFP
                     GSTLLTRTAGATTLILLPFFLVGLWACWRNRHSGGMVLVFIVGTMLIGLIFWADTRIR
                     APAEPFIVLAAAIGAVWWAGRKAERRMKIDEPLPAPPEQEGSG"
     gene            complement(3038..4246)
                     /locus_tag="VFH60_11270"
     CDS             complement(3038..4246)
                     /locus_tag="VFH60_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6314408.1"
                     /translation="MKVLMIVQEPTVTRRILQEARSLIDAGHSVTIMSRPAGAEDTTE
                     EIDGIPVEWVAVRGRDPRFGWLYRLAGVSLGSNFAALWSVLTMKHTFTLRALDMAVAA
                     KADVYHSHDLNNLELACTAAAANGARVVYDSHELFTEIGNRWIKLRRTAWMQLERRLI
                     KKVDLVVTVNEFIAAELSSRYGVPAPLVVMNCPDPPPDFDPSTPYDLLRERLGLAPGR
                     KIVLYQGWMSEGRGLENLVRCAPMLAGDAAVVFIGYGDYQQVLERMGTAEAPGRVYFL
                     PAVSHRDLLPYCASADVGLIPYQAVDLNNYYSSPNKLFDYIQAGLPIVASDFPFLRKV
                     IATDDLGVVADLSTPESYARAINQVLTLPDGGEQIRANMRRVASRYTWQSQARALVAA
                     YEALQAGGGR"
     gene            complement(4243..>4649)
                     /locus_tag="VFH60_11275"
     CDS             complement(4243..>4649)
                     /locus_tag="VFH60_11275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6314409.1"
                     /translation="EALARRLGVNGVCHFHGALPHEQVAAMMPSYDVLVLSSLAETFG
                     VVAVEALASGVPVVATRCGGPEEVLTPDLGVLVEKADPEALADGILDVMRRPHDFPPE
                     HLRRVVEERFGEARVTGRLVSLYRQVGEARPA"
BASE COUNT          893 a         1603 c         1410 g          743 t
ORIGIN      
        1 atactgaagc aacaactcaa aactcacatt caaaactcaa aactccaact agaccaggag
       61 caactgctta tgagcgacca gcaaagcaca cagtccctgc actcggccga agacctcggt
      121 tcgcccactc ccaccgagcc gggggccgat ggctccctgg agggcagcaa cgggtccggc
      181 gggggtagcc ccaccgtggc tatgggcggg ggtactccca ccgtctctac cgccaccggc
      241 acgaccgaaa tccccaagcc acctaacggc gcgaggcccg acgtgctcct ggaagtggac
      301 cgcctggtga agtacttccc cgtgcgcggc ggcctcttgc agcgcaccgt cgcctggacc
      361 aaggcggtag acgacgttag cttcttcatc cgccagggcg aaacgctcgg cctcgtggga
      421 gagtcgggct gcggcaagac cacggtggga cgcacgttgc tccgcctcac cccggccacc
      481 gggggcgagg tgcgcttcca ggggcagaac atcttcgacc tcaaggccga ccatctgaag
      541 cgggtccgca gggatatgca gatcatcttc caggacccct tctcctcgct cgacccgcgc
      601 gtgcccatcg gtgaaagcat aggtgagggc ctcatcatcc acggcgtgtc caagaacgag
      661 cgccaggacc gtgtgtacga cgcgttggca cacgtgggcc tgctccccga acacgccaag
      721 cgctacccgc acgagttctc aggcggccag aggcagcgta tcggtatcgc ccgcgccctc
      781 gtgctgcgcc cgaagttcat cgtgtgcgac gagccggtgt cggccctcga cgtgtctatc
      841 cagtcgcagg tgctcaacat cttgaaggac ttgcaggggg agatgggcct tacctatctc
      901 ttcatcgccc acaacctgag cgtggtggaa cacatctccg accgcgtagg cgtgatgtac
      961 ctgggcaaga tggccgagct tgcggagcgc accgccctgt tcgcaagccc ccagcacccc
     1021 tacacccacg cactgctgtc ggccatacca atccccgacc cgaagatcag gcgcgagcgc
     1081 atcatcctca agggagacgt gccttcgccg ctcaacccgc ctaccggctg ccggttccac
     1141 actcggtgct ggctagctca agacatctgc aagcagcagg tgccagaatg gcgcaacgtg
     1201 gcccctcccg gccagctaga gcacttcgtg gcctgccact tcgccccgtt caccaagatg
     1261 ccgctgccgg gcgaaactag ctccatcatc ggcaaggacg aaacgacggt agtgcagcac
     1321 cagtaattga caaagcacaa aaggagggga gaccgcgcgg tctcccctcc tttttgcttc
     1381 cttgaattac gttgtggttg ctgactgtca agcgccgctt ccggacagaa gattagctca
     1441 acctgagcct tcctgttctg gtggggcagg tagaggttcg tctatcttca tgcggcgttc
     1501 cgctttgcgc cctgcccacc ataccgcccc gattgctgcc gcaagcacga tgaacggctc
     1561 ggcaggagcg cggatgcggg tgtcggccca gaaaatcagc ccgatgagca tcgtccccac
     1621 tatgaacacc agcaccatgc cgccactgtg cctgttacgc cagcacgccc acaggcccac
     1681 caggaagaaa ggcagcaaga tgagtgtagt agcgcccgcg gtcctcgtca agagggtcga
     1741 gccggggaag acgttccaga aacggaggaa cgtgccgagc agcagcaccg gcacgtcctg
     1801 cgggtgcgac gtgaggtatc gcaggccgta ctcgtagccc ttcctgtcga gtaatccagg
     1861 gttgcctgta tgggaaatgt actcagcgta gtcgggcagc atctcggcgg gcacgtagcg
     1921 tccccaccgc tcgccggggc gggccgtgaa ctcgttgttg cttatccaga agacgtaccc
     1981 tcccgccgac gagccgtagc cggaggtcaa ggggatgaac tgcccgtatt cagcgaagtt
     2041 gcgggccacc cacggcgata aggccactaa ggccccggcc agcaacagcg cgggcctcac
     2101 cagcctgcca cgcccccgcg acacgagcag cagccacagc acaagcccca ccgccaccag
     2161 gatggccgta ggccgtgtca gcagcgccac gcccagcacc ccccctcccg ccagcagccg
     2221 ccatgccccg gcacgctccg ggcagaccag gagggcggcc agcacggcgt acacgaacac
     2281 gagcaggttc tcggtcgcca ggtaccggct ccagaagatg ctgaacgggt ggaacgcgaa
     2341 cacgtgcgcg gccagcatgc ctacggagcg actccagccc ctgctcccga cgtagtaaag
     2401 gaggggcacc acagccacaa gcagggcgac ctgcaccagc cgggcaacgg cgggctgcac
     2461 cccaaagacg ctgtacacca ggaacagcag gacggggtag ccgggcgagc ggttgacctg
     2521 tggctggccg tccaggctgt agccgcgccc cgatagcagg ctcttcgcca gctcgttgta
     2581 gtcgagctcg tcctcctgtg ggggcgcgaa cagcccgccg gaggctacca gccaacccac
     2641 ccgcagcgca aaccccacca gcaccagcgc gccaagcacc agccccggcc tggacagcgt
     2701 ccaaccccag agcgaccgta tggcgtggga aagcctggac agcaactgct gcctcctact
     2761 tcttattact ggtgtctacg tgcacagtgc cgcctgtgcg agaccaaagt ggcccctgtc
     2821 attctgagcg tggcgaagaa tctaaagaag gtggcacagg gaggctgttg aactagacgg
     2881 ctcatggtgc tgatattcaa cgcaggggca aagccagcat cttagattct tcgctacgct
     2941 cagaatgaca ggggccacct ctggtcagtt gtctggtaca gtctgccgat actccccagt
     3001 ccgggcatct gcgattagtg caatcgagcg ggctgcatca gcggccgccc cccgcctgca
     3061 acgcttcgta ggccgcaacg agggcgcggg cctggctctg ccacgtgtag cgggaggcca
     3121 cacggcgcat gttggccctt atctgctcgc caccgtccgg cagcgtcagc acctggttga
     3181 tggccctggc gtagctctcc ggcgtgctca gatcggccac cactccgaga tcgtcggtcg
     3241 cgatcacctt gcgcaggaag gggaagtcgc tggctactat gggcagaccc gcctgtatgt
     3301 agtcgaatag cttgttgggt gacgagtagt agttgttcag gtcaaccgcc tggtagggga
     3361 taaggcccac gtccgcagag gcgcaataag gcagcaagtc ccggtgggat accgccggga
     3421 ggaagtagac ccgtccgggc gcttcggccg tccccatgcg ctcgagcacc tgctggtaat
     3481 cgccgtagcc tatgaacacc acggcggcgt caccggccag catgggcgca caccgcacca
     3541 ggttctccag cccgcgcccc tcagacatcc agccctggta gagcacaatc ttcctgccgg
     3601 gggccaggcc caggcgctcg cggagtaggt cgtagggtgt cgatggatcg aagtcgggcg
     3661 gcgggtcggg gcagttcatc accacgaggg gggcgggcac gccgtagcgg ctcgatagct
     3721 cggcggctat gaactcgttt accgtgacta ccaggtccac cttcttgata agcctgcgct
     3781 ccaactgcat ccaggccgtg cgccgcagct ttatccagcg gttgccgatc tccgtgaaaa
     3841 gctcgtgcga gtcgtacacc acgcgcgccc cgttggcggc ggctgccgtg caggcaagct
     3901 ccaggttgtt caggtcatgg gagtggtaca cgtcggcctt cgccgccacg gccatgtcga
     3961 gcgccctcag ggtgaaagtg tgcttcatgg tgagcacgct ccagagggcc gcgaagttgc
     4021 tgcccaacga cacccccgcc agccggtaga gccagccgaa gcgcgggtct cggcctctca
     4081 ccgccaccca ctccaccggg atgccgtcta tctcctcggt ggtatcctcg gccccggcgg
     4141 ggcgggacat tatagtgacg ctatgcccgg cgtctatgag gctgcgcgct tcctgtagga
     4201 tgcggcgcgt cacagttggc tcctggacga tcatcagcac cttcatgcgg gccgcgcctc
     4261 cccgacctgc cggtagaggc ttaccagccg ccccgtaacc ctggcctcgc caaagcgctc
     4321 ctccaccacc cgccgcaggt gttccggagg gaaatcgtgc ggcctgcgca tcacgtccaa
     4381 tatgccatcg gccagcgcct ccgggtcggc tttctccacg agaaccccca gatcaggcgt
     4441 cagcacctcc tccggtccgc cgcacctagt cgccacgacc ggcaccccgg aagccagcgc
     4501 ctccaccgcc actaccccga acgtctcggc cagggaggag agcaccaaca catcatacga
     4561 gggcatcatg gcggccacct gctcgtgggg cagcgcgccg tggaagtggc acaccccgtt
     4621 cacgcccagc cgccgggcca gcgcctcgt
//
LOCUS       DASRWZ010000715         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_674963, whole genome shotgun sequence.
ACCESSION   DASRWZ010000715 DASRWZ010000000
VERSION     DASRWZ010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674963"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..2493
                     /locus_tag="VFH60_11280"
     CDS             154..2493
                     /locus_tag="VFH60_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET6314410.1"
                     /translation="MPNHAARYRVPSRPVTTPGRHPLVHGRRERLACFAAMLLMLLGL
                     GTWAIVASPPAAAAPQAAPKSYPVRPYVYAASTSATTYDVMLADGVSKDRMVAQVKVD
                     SIYFGDVSARLAYDRGMFAFRVSGDRNGGSSLYVVDIKSGKSTQIALAKSKAEGIGAF
                     AWSPAGRTMAYVRSSPATDPADADLAYGTVYLYSAGFNPVRLGGSNGNDRVLAFSGDG
                     LGVYVARREAARAGTLEHLAYLPLSGGDATVVMRSQPGLKYSRYAVSALPNEPARIAA
                     LAEGDFTLALTGTLPGRPTATPTPTSTALPQSTITSTFAAAASATLAATTPKATATPT
                     PTVPASKVPVAGKLARPSGLGIVVLDQVSAVPYLVRRDAEAFPYVAWAADGKSLLVGG
                     GRSGLAWVVDLEGGRRALGTPLTGLSVLSWSEDGSIAMLSDSPTSKLLALDFRGGQVL
                     ASRALGFSKVAAPAVALKVPYIHQVNDTAASGDGNWACGPTSVAMALAYYGKIEPWET
                     VMQGRTGAAAPSDSTRPLSGIDFAPYVTNVYTNNGRTYSATARDPRGNMLAGLYGTIC
                     PTGLASWSAMRSVLEWHGLQATQIAASWDGVVAALKRGHPVLLGNMLTSEGHILRVVG
                     YTPDGNLIVNDPYGNRFAPGYGANNGEQVVYAWKRTTARTAVEVTGIYPPPTRTPTPT
                     ATSTVTPTNTPIPTATNTPTPVVTFIAIPDAQSNEGALQQSTPEATQEPTQEPTFEPT
                     LEPTQEPTPIPTSEPTQEPTQEPPTPLPALEPVPVTPAP"
BASE COUNT          532 a         1034 c          972 g          552 t
ORIGIN      
        1 atctaaaatc caaaatggga aaatcgttac caaagtcctc ttgacgctcg tctgctacat
       61 cgggtaagat cgattcattg tctgcgtgag agagcgcgga cgtccgcacc gccgagtccg
      121 aatcccccct tagacgttag attgggggct tagttgccca accacgccgc gcggtaccgc
      181 gttccatcca ggcctgtaac tacgccaggc aggcaccccc tcgttcatgg ccgccgggag
      241 cggctggcgt gctttgccgc catgttgctg atgctgctcg gcctcggcac gtgggcgatt
      301 gtcgcttctc cacccgccgc agccgctccc caggccgcgc ccaagtcgta ccctgtgcgg
      361 ccctacgtgt acgctgcctc tacgtcggcc accacctacg atgttatgct tgccgatggg
      421 gtgagcaaag accgcatggt ggcccaggtc aaggtggaca gcatctactt tggcgacgta
      481 tcggcccggc ttgcttacga ccggggcatg tttgccttcc gggtgagcgg ggaccgcaac
      541 ggcggctcaa gcctgtacgt cgttgacata aagagtggca agtccactca gatcgcgctg
      601 gctaagagca aagccgaggg aatcggggcg ttcgcgtggt cgcccgccgg taggactatg
      661 gcctacgttc gcagctcccc cgccaccgac cccgcggacg ccgacctggc ttacggcacc
      721 gtctacctct attcggcggg cttcaacccg gtcaggctgg gcggctccaa cggcaacgac
      781 cgcgtgttgg cgttctccgg ggatggcctg ggcgtgtacg tggcgaggcg ggaggctgcc
      841 agggcgggca ctttggagca cctggcgtac ctgcccctgt cgggcgggga tgccacggta
      901 gtcatgcgca gccaacccgg cctcaagtac tcccgctatg ctgtgtcggc gctgcccaac
      961 gagcctgcca ggattgcggc gttggccgag ggcgatttca cactggcgct caccggcacc
     1021 ctccccggca ggcccacggc tactcccact ccgacctcaa cggcgctccc gcagagcact
     1081 atcacctcta ccttcgcggc ggcagcctcg gctacattgg ccgccaccac cccgaaggca
     1141 accgccacgc cgacccctac cgttccagcc tccaaagtgc cggtagcggg caagttggcg
     1201 cggccttcgg ggctgggcat cgtggtgctc gaccaggtca gcgcggtacc ctacctcgtg
     1261 cggcgcgacg ctgaagcttt cccctatgtg gcgtgggcag ccgatggcaa gtcgctgctc
     1321 gtgggcggcg ggcgctccgg cttggcgtgg gtggtggacc ttgaaggggg caggcgcgcc
     1381 ctgggcaccc ctctgacggg gctttcggtg ctgtcctggt ccgaagacgg ctctatcgct
     1441 atgctgtccg actcgcccac gagcaagctg ctggcgctcg acttcagggg cggccaggtg
     1501 ctggcatcgc gcgcgctggg cttctcgaag gtggccgcgc ccgccgtggc cctcaaggtg
     1561 ccttacatcc accaggtgaa cgacaccgcc gcgagcggag acggcaactg ggcgtgcggc
     1621 cctacgtctg tggcgatggc gctggcttat tacggcaaga tagagccgtg ggagacggtc
     1681 atgcaggggc gcaccggggc ggctgcccct tccgactcga caaggcccct ctcgggcatt
     1741 gacttcgcgc cgtacgtcac caacgtctac accaataacg gccgtaccta cagcgccacg
     1801 gctcgcgacc cacgcggcaa catgctggcg ggcctgtatg gcacaatctg ccccaccggg
     1861 ttggcaagct ggagtgccat gcggtcggtg ctggaatggc acgggctgca agccacgcag
     1921 atagcggcta gctgggacgg cgtggtggcg gccctcaagc gggggcaccc ggtgctgctg
     1981 ggcaacatgc tcacgtccga ggggcacatc ctgcgggtgg tgggctacac gccggacggc
     2041 aacctgattg tgaacgaccc ttacggcaac cgcttcgcac cgggctatgg cgcgaacaac
     2101 ggcgagcagg tggtctacgc gtggaagcgc accactgccc gcaccgcagt ggaagtcacc
     2161 ggcatctacc caccgccgac caggacgccc acgcccaccg ctacgagcac ggtcacaccc
     2221 accaacacgc ctataccgac ggccacaaat acccccacgc cggtagtgac tttcatagcg
     2281 ataccggacg ctcagtccaa tgaaggggcg ctgcaacagt ccacgccgga ggccacacag
     2341 gagccgacgc aggagcccac tttcgagcca acgctggaac ctactcagga gccaacgccg
     2401 ataccaacgt cggagcctac tcaggagccg acgcaggagc cgcctactcc gctgcccgcg
     2461 ttggaacctg tacccgttac gcctgctcct tagggcctgt gactttgggg cggggtggcc
     2521 ttacacggac gccctgcatg atcgccctgt ggctaaagcc acgggctggg aagtagcgaa
     2581 gtctccttcg gagactgggg ctcacctgca aactgtaggc gatagccgaa caaagagaga
     2641 ccttgtcatt ctgagcgcag cgaagaatct cgtccctcac cgggatgtgg cccttttcta
     2701 ttgcaccatt gcaccttata gtcgtaatga aagatggtat ttgggtgccg caggacgaga
     2761 ttcttcgctg cgctcagaat gacagagggg cacaggttac cgcactggcg gcactttgca
     2821 gatagaccca gcccccgtca aggagagtca ccgtagggga ctttgcccct tgtcagcccg
     2881 tggctttagc cacagggcgg tggtggcggg gtatcctata gtcccgcgtc tactttgccg
     2941 ctgctgccgc tgccgcctcc gcttcagcct cttcgtgctt cagccgcctg tgctctttgt
     3001 agggcagcac ttccagcact tcgcccgcgt tgtggcgcgc ctgtaggccg ttccagaagt
     3061 cggcggtgaa cagctcgccg tggtgctcta
//
LOCUS       DASRWZ010000716         3294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1292121, whole genome shotgun sequence.
ACCESSION   DASRWZ010000716 DASRWZ010000000
VERSION     DASRWZ010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292121"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1348
                     /locus_tag="VFH60_11285"
     CDS             26..1348
                     /locus_tag="VFH60_11285"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6314411.1"
                     /translation="MRRVGSMFGWRFGKYGEIFSVRAFRLFWSGFTFSVLGDAMSRVA
                     LTWFVYEATGSAEALGWLMLAYTGPVILGGLVAGTLLDRFDRRKVMLADNLVRGLAVA
                     LVPALHWLGLLQLWHVYAVAAIYGSLMMVSLAGGPALVPSLVRKEQLATANALEMLTF
                     TLGGVVGPVLAGLLISSIGATNVLVLDALSYGVFALALSRIRFTGGEGGAPAAASAEG
                     DRPNQTLLGVVRFAVGDRVLLSTTLMFAAFNLGQGFLFVWLPVLSGNMSGGGPELYGM
                     LLGAWAAGEVIGSLLVGSVTLKVPLGTLICGSQFLSGLSLGLLVVDQTLWFSVPALAM
                     LGLFSAPLTIWAQTLRMQVIPPDMRGRTFALLRTMMQSTPPIGGAIAGLLLPVLGIPM
                     MVAVSAALAGLPGLLGYGVAELRGAGASEQTLQHEMPVPGDIDYAIGE"
     gene            1345..1953
                     /locus_tag="VFH60_11290"
     CDS             1345..1953
                     /locus_tag="VFH60_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6314412.1"
                     /translation="MTADMGDNHNALTGFHTEALDVNNWPDLERLFGARGACGGCWCM
                     WWVLPRKQYDAQKGEANKQALRARVEAGEVPGLLGYIGDEPVAWCAVQPRQAYPALER
                     SRTLARVDEQPVWSIVCLFVARKYRRKGISTLLLASGVEHAGRQGASIVEGYPVEPRS
                     EKMPDAFAWTGTLTAFRRAGFVEVARRSETRPIVRYLRQATD"
     gene            1982..2488
                     /locus_tag="VFH60_11295"
     CDS             1982..2488
                     /locus_tag="VFH60_11295"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6314413.1"
                     /translation="MAIDVDTISDPQVRQFFRVYLPHRQVVRDFYELLPEDKLTYRMV
                     DTGGRRSDSPHESLVHILETQLAYFEGIKSGSLSFDAMGAERYAAMSKRQLLDELTAL
                     DEEMYRHVGRPAFDPQTRVETSWGDETALGVLALVRDHDILHAGWNLALMDHLGMERY
                     QSLVDIWG"
     gene            2741..2974
                     /locus_tag="VFH60_11300"
     CDS             2741..2974
                     /locus_tag="VFH60_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314414.1"
                     /translation="MAQVDINDLVEYQVFRRNPGPLLARAAEGRPLLLLKGRTRFVIV
                     DAESYNSLVRRAADGEHPAEPGAEGSGLEEGAA"
     gene            2971..>3294
                     /locus_tag="VFH60_11305"
     CDS             2971..>3294
                     /locus_tag="VFH60_11305"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6314415.1"
                     /translation="MIIAVSQHKGGTGKTSTVLNLGAAIAEKGHRVLLVDLDPQADLS
                     AGLGIEVDLDEKSPRPTLYTVLAEEQGTLAGLVAPAGLERLSLVPGTLDMADLELHLA
                     PQIGRE"
BASE COUNT          578 a          961 c         1148 g          607 t
ORIGIN      
        1 agggtcaatt tggatcttga gtggcatgcg gagggttggg tcgatgttcg gctggcggtt
       61 tggcaagtac ggagaaatct tcagcgtgcg ggcgttccgg ctattttggt cgggcttcac
      121 cttctcagtg ctgggagatg ctatgtctcg ggtggccctc acgtggttcg tgtacgaggc
      181 tacaggctcg gcggaggcgt tgggctggct gatgctggcc tataccggcc cggtgatctt
      241 aggcgggctg gtggcaggca cgctcctgga caggttcgac aggcggaagg tcatgcttgc
      301 ggacaacctg gtgcgcgggc tggccgtggc cctggtgccc gccttgcact ggcttgggtt
      361 attgcagctc tggcacgtct acgcggtagc cgcaatctac ggcagcctga tgatggtgtc
      421 cctggccgga gggcctgccc tggtaccatc gttggtacga aaggagcagc tagccaccgc
      481 taacgccctt gagatgctta ccttcacgct tggaggggta gtggggccgg tgctggcagg
      541 gctgctgatc agttccatag gtgcgaccaa tgtgcttgtg ctcgacgccc tctcctatgg
      601 ggtgttcgcc ctggccctct cacgcataag gtttaccggc ggtgaggggg gagctccggc
      661 tgccgccagt gccgaggggg acaggcccaa ccagactctg ttgggtgtgg tacgcttcgc
      721 ggtaggggac cgggtgctcc tctcgaccac gctcatgttc gcggctttca acctgggcca
      781 gggcttcctc ttcgtgtggc tgcctgtgct ctccggcaat atgtcagggg gtgggccaga
      841 actgtacggc atgttgctgg gcgcgtgggc ggccggcgag gtgatcggct cgctactggt
      901 cgggagcgtg acgctaaagg tgccgctggg cacgttgatc tgcgggtcgc agttcctctc
      961 cggcctgtcg ctgggcctgt tggttgtaga tcagacgctc tggttttcgg tgcccgcgct
     1021 ggcgatgctg ggcctattca gcgcgccgct cacgatctgg gcgcagacgt tgcgcatgca
     1081 agtcatccca ccggacatgc gcggacgcac cttcgccctg ctacggacca tgatgcagag
     1141 cactccgcct atcgggggtg ccatcgccgg cctcctgctg ccggtgctcg gcatccccat
     1201 gatggtcgcg gtctcggcgg cgctggcggg attaccaggg ctactcggat acggggtggc
     1261 cgagcttaga ggagctgggg cctccgagca gaccctccag cacgagatgc cggtgccggg
     1321 tgacatagat tacgcaatag gagagtgacc gcagacatgg gggacaacca caatgcgctt
     1381 acaggcttcc acaccgaagc cctggatgta aataactggc cggacctgga gcgactgttc
     1441 ggagcgcgtg gcgcgtgcgg cggctgttgg tgcatgtggt gggtactgcc gcgtaagcaa
     1501 tacgacgcac agaaggggga agccaacaaa caggctttgc gggcgcgggt cgaggcgggc
     1561 gaggtgccgg gcctgctggg ttacatcggg gacgagccgg tagcatggtg cgccgtgcag
     1621 ccacgccagg catacccggc gctggaacgc tcccgcacgc tggcgcgggt ggacgagcag
     1681 cccgtgtggt cgatcgtgtg cctgttcgtg gcgagaaagt acaggcgcaa gggcatctct
     1741 acactgctgc tggcaagcgg ggtcgagcat gcgggccggc agggtgcctc catcgtcgag
     1801 gggtacccgg tagaaccgcg gagcgagaag atgcccgatg cgttcgcctg gaccggcacc
     1861 cttacggcct ttcgccgggc gggcttcgtg gaggtagcca ggcgttccga aacacgcccc
     1921 atcgtgcggt atctcagaca agctacggac tagcaagcaa aatgttcaag aggagatgag
     1981 aatggctata gacgtagata caatcagcga cccacaggtg agacagttct tccgggtcta
     2041 cctcccacac cggcaggtgg tccgcgactt ctatgagttg cttcccgagg acaagctcac
     2101 ctacaggatg gtagacaccg gaggcaggcg ttccgactcg ccccatgaga gcctggtgca
     2161 catcctggag acacagcttg cgtacttcga gggcatcaag tcgggcagcc tgagtttcga
     2221 cgcgatgggc gcggagcggt acgccgctat gagcaagcgg caactactgg acgagcttac
     2281 cgcgctggac gaggagatgt accgccacgt cggccgcccg gcgtttgacc cccagacgag
     2341 ggtggagacc tcgtggggag acgagaccgc cctgggagtg ctagccctgg tccgcgacca
     2401 cgacatcctg catgcgggct ggaatctcgc cctcatggac cacctcggca tggagcggta
     2461 ccaatcgctg gtggatatct ggggctaaag ctacaccgga acataatcgc atacgaccaa
     2521 accggatgta agggcaagcg tgtcccttac atccggtttt tgctacaagc tgcggctggg
     2581 ccacccattg tagcaagcct acgtgcaagg tgcagaaccg ggtattgcgg gccgccaatg
     2641 gctacaataa ctgcgctgga atccagagtc ctttttcggc tacgcatgtc agagaaggtc
     2701 ggcctcacga ggtgcgagct taccggagac aggaggtcag atggcccagg tagacatcaa
     2761 cgatctagtc gagtaccagg tgttccggcg caatcccggc cccctgctcg cccgcgccgc
     2821 cgagggtcgg cccctgttgc tgctcaaagg ccgcacccgc ttcgtgatag tggatgccga
     2881 gtcgtacaat agccttgtgc ggcgggcggc ggacggagag caccccgccg agccgggggc
     2941 ggagggtagc ggcctcgagg agggtgcggc atgattatcg ccgtgagcca acacaagggc
     3001 ggcaccggca agacctccac cgtgctcaac ctgggtgcgg ccatcgccga gaagggccac
     3061 cgagtgctgc tggtggacct cgacccacag gccgacctct cggcggggct gggcatagag
     3121 gtcgatctgg acgagaaaag cccccgcccc accctgtaca cggtgctggc cgaggagcag
     3181 ggcacgctgg cggggctggt cgcgccagcg gggctggagc ggttgagcct tgtgccaggc
     3241 acgctcgaca tggccgacct ggagttgcac ctggcacccc aaatcggcag agag
//
LOCUS       DASRWZ010000717         1764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_675042, whole genome shotgun sequence.
ACCESSION   DASRWZ010000717 DASRWZ010000000
VERSION     DASRWZ010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675042"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="VFH60_11310"
     CDS             complement(<1..1655)
                     /locus_tag="VFH60_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET6314416.1"
                     /translation="MSRLHSARAARTLFALLLLLTSSVVYRPSFARSGPTAAQPTTAF
                     VTVQGNQLMLRGVPIKLKGTNFYPKDRPWADMWKKWNGPETRTDLARAREIGVNSVRI
                     LVPYKPVNGWTDRDTGAVVPEYLDRLRQMVQMAGELDMKVIIALFDFYDPEDDRRAGM
                     QPSETEARNLRYLRDIVPAFAGDDRVLAWDLHNEPEHYKTWQEVKKPQVMIAWMARMA
                     AEIRRLDPNHLLTVGMADYKNLFTADDTGAPYEEEPARGLTPADLSDFLSWHSYNAGD
                     MDWQIRYIRAHSNKPLVLQETGWPSGPACTAPEYNEQHQTFLYNTMVDWANKGGLSGL
                     MQWQLWDLPLGGSVGAGRESHEDFFGLLRRDGSWKAAMPVFRDGWKVEPLPSVTRTAL
                     PLTVFVPNPTPPDPNYQPPLYFPETGKYIHAEFRDYWRRFGGLAVFGYPITDQRQEDD
                     MWVQYFERARFEFHPEVRREVKDYDKLDKVQQYKLIVQLTRLGAELVDQKTGGKGFPL
                     PDRATLPAGATVFPETGHSLSGKIGKYWWANNGLTNFGFPLSEP"
BASE COUNT          294 a          521 c          613 g          336 t
ORIGIN      
        1 ggctcggaga gagggaagcc gaagttggtc aggccgttgt tggcccacca gtacttgcca
       61 atcttgcctg aaagcgagtg tccggtctca gggaacacag tagcgcccgc aggtaaagtg
      121 gcacgatcgg gcagggggaa gcctttgccg cccgttttct ggtccaccag ttcggcaccc
      181 agccgtgtga gttgcacgat gagcttgtac tgctgcactt tgtccagctt gtcatagtcc
      241 ttcacctcgc gccgcacctc cgggtggaac tcgaaccgcg cgcgctcgaa gtactgcacc
      301 cacatgtcgt cctcctggcg ctgatccgtt atcgggtagc cgaacaccgc gagaccgcca
      361 aagcgccgcc agtagtcgcg gaactcggcg tggatgtact tgccggtctc tgggaagtag
      421 agcggcggct ggtagttggg gtcgggcggg gtgggattgg gcacgaacac ggtgaggggc
      481 agggcggtcc tggtgacgct gggcagcggc tccaccttcc agccgtcccg gaatacgggc
      541 atggcggcct tccacgagcc gtcgcgcctc agcaggccaa agaagtcctc gtgcgactcg
      601 cggcccgcgc ccacgctccc acccagcggc aggtcccaaa gctgccactg catcaggccc
      661 gacaggccgc ccttgttcgc ccaatctacc atcgtgttgt agaggaacgt ctggtgctgc
      721 tcgttgtatt cgggggcggt gcaggccggg ccgctgggcc agcctgtctc ctgcaacacc
      781 aggggtttgt tcgaatgggc gcgtatgtag cgtatctgcc agtccatatc gcctgcgttg
      841 tagctgtgcc agctaaggaa atcgctcaag tcggcggggg tgaggccgcg cgcaggctcc
      901 tcctcgtagg gcgcgccggt gtcgtcggcg gtgaagaggt tcttgtagtc ggccatgccc
      961 acggtgagca ggtggttggg gtcgaggcgg cgaatttcgg cggccatgcg cgccatccag
     1021 gcaatcatca cctggggctt cttcacctcc tgccaggtct tgtagtgctc aggctcgttg
     1081 tgcaggtccc aggcgagcac gcggtcgtcg cctgcgaagg cgggcacgat gtcgcgcagg
     1141 tagcgcaggt tgcgggcctc ggtctcggag ggttgcatgc cggcacgcct gtcgtcctcc
     1201 gggtcgtaga agtcgaacag ggcgatgatc accttcatgt caagctcgcc cgccatctgc
     1261 accatctggc gcagccggtc caggtactcg ggcaccaccg cgccggtgtc cctgtcggtc
     1321 cagccgttca ccggcttgta gggcaccagg atgcgcaccg aattgacccc gatttcgcgg
     1381 gcgcgggcga ggtcggtgcg ggtctcaggc ccgttccact tcttccacat gtcggcccac
     1441 gggcggtcct tggggtagaa gttggtgcct ttcagcttga tgggcacgcc ccgcagcatg
     1501 agctggttgc cctggacggt gacgaaggcg gtggtcggct gggcggcggt gggtcctgag
     1561 cgtgcgaagg atggacgata gacgacggac gacgtgagga gcaggagcag ggcgaacaga
     1621 gtgcgcgcag ctcttgcgct gtggagcctg gacatgaggg acaaggtgcc tttctagagg
     1681 gcgggacgtg ctacggtata gctatttcta ccaggaaata cgggtgaata gcaagattgg
     1741 agcgggcaaa acgataatca gcga
//
LOCUS       DASRWZ010000718         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_743717, whole genome shotgun sequence.
ACCESSION   DASRWZ010000718 DASRWZ010000000
VERSION     DASRWZ010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743717"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VFH60_11315"
     CDS             complement(<1..877)
                     /locus_tag="VFH60_11315"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET6314417.1"
                     /translation="MSSTVAARPSDATRPVGGRLSVALARLEGHAPRLLVLGVVLWIA
                     VMFVASYYKYETFGQGYDQVDYEQGIWNTLQGRVMEDSRFNFTGSVFGMDWMPMLLFF
                     VPVYALVPSAHVLFFLQILGSALGAVPVYWLARDRLGVKLAAAGAAMAYLLYPTLLHT
                     ALNPFQVRLFALTLLLFGFYFYERGNWKLFLACALMAVLARTDVALVVMMFALYGLLT
                     RRKWQYVVPPLVVGLGYFALSTFVITPSFAYPGALTPPPPGTQLDMSCWPCGMSPQLA
                     YYAHLGRSGPEILQFV"
     gene            complement(874..1650)
                     /locus_tag="VFH60_11320"
     CDS             complement(874..1650)
                     /locus_tag="VFH60_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6314418.1"
                     /translation="MRQDLYYDLYIKEQDYWWHVGKRAIVYSLLDRYLPPARGRERHA
                     LDLGCGTGLNLDHLARYARPTGTDFSEEALAFCRERGHTSLAKADAAELPFPDGYFDI
                     ITALDVIEHLDDEHAALTELKRVLRPGGLLIVSVPAYKALWTYWDDILGHRRRYTTGM
                     MRKAMHKAGLRVRKVSYSNALILAPVVLVRLLKTLLQKRAQLRNENKDPETDFMPVPG
                     WVNRLLIKYYQGEAGFISRSRLPFGLSVVCVAQKGRENGR"
     gene            complement(1655..>3475)
                     /locus_tag="VFH60_11325"
     CDS             complement(1655..>3475)
                     /locus_tag="VFH60_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314419.1"
                     /translation="DAYVSGFHAKEATPGFEYRWSTSRAVVNFPEIGNEPVNVSITHV
                     GAANDLAAKVVTVTIRGQQFAMPSPPELRTDTFFVPRGDFMQGSLNVVMDVDTFNEGT
                     AENPGRALGVNIDSVEVAPAEYGLRPVVLPSPGALLALALGLLLFGASAAFATGRTLP
                     ALVVGGALSFATVVLILVARPELGLLLPSILPMGAWCLGFAVVGRLGLGLLTGSSTRA
                     SRLTIGLGTAAFVLAFALRFGGLTYPQFLTSDLLLNVHNVQALLGGEWIMSEPLPDGT
                     PAPYPPALYVLVTPFTWVFGSGDEAISLALKWMCSLLDSLTCLALAWCGYRLWGGASG
                     AWAALLYAVSVAPFDLFSAGNYTNLFAQSTLNLSILGGLVYLSVREEGSRRHAWVRAP
                     VAACFFLTLLGHYGMMIGALAIAGLFLLWMVAGLWLGWGSDRGWRLIRLISFSVAVGF
                     VTYYRNVLDLVANHFGGLLGRLTGAKATEGAATPQAFSLDKLSRKVAYLVGPAQVLAA
                     ATAAVVGLRLNRVASGWLGAWLVVGVVFALLDQTLGDAIRWYYMVAAAVSLLAGRTLG
                     LLAERGRIGRWLAALLVAALLWQLLNTWVGDMIFYRYHTR"
BASE COUNT          695 a         1163 c         1115 g          502 t
ORIGIN      
        1 acacgaattg caagatctca ggcccgctcc ggcccaggtg ggcgtagtag gcaagctgcg
       61 gactcatgcc gcacggccag cacgacatat ctagctgcgt gcctggaggg ggcggggtca
      121 gcgcgccggg ataggcgaac gagggcgtaa tgacgaaggt agacagcgcg aagtagccta
      181 gccccacgac caggggaggc acgacatact gccacttgcg ccgcgtaagc aatccgtaaa
      241 gcgcgaacat catcaccacc agcgccacgt cggtgcgggc cagcaccgcc ataagcgcgc
      301 aagccaggaa aagcttccag ttgcctcgct cgtagaagta gaagccgaac agcagcaggg
      361 tcagcgcgaa aagccgcacc tggaacgggt tgagggccgt gtgcaggagc gtcgggtaaa
      421 gtagatatgc catcgccgcg cccgctgccg ccagcttcac gcccaggcgg tcgcgcgcca
      481 gccagtacac gggcaccgcg cccaacgcgg atcccagtat ttgcaggaag aagagcacgt
      541 gggccgacgg cacgagcgcg tacaccggca cgaagaagag cagcatcggc atccagtcca
      601 tgccgaacac cgagccggtg aaattgaagc gcgagtcctc catgacccgg ccctgcaggg
      661 tgttccagat gccctgctcg tagtctacct ggtcgtagcc ctggccgaac gtctcgtact
      721 tgtagtagct ggctacgaac atgaccgcta tccagagcac tacgccaagc accagcaagc
      781 gtggggcatg cccctcaagc cgcgccagcg ccacgctaag gcggccccct accggccggg
      841 tagcgtcact gggtcgtgcg gctaccgtgc tgctcaacgc ccgttctccc ggcccttctg
      901 agcgacgcac accaccgaca gcccaaacgg caggcgagag cggctgatga agccggcctc
      961 cccctggtag tacttgatga gcagcctgtt cacccagccg ggcaccggca tgaagtctgt
     1021 ttcggggtcc ttgttctcgt tgcgaagctg agcccgtttc tgcaatagcg tcttcagcag
     1081 gcgcacgagc accaccgggg cgaggatgag ggcgttcgag tagctcacct tgcgcacccg
     1141 cagcccggcc ttgtgcatcg ccttacgcat catgccggtg gtgtagcggc ggcggtggcc
     1201 caggatgtcg tcccagtacg tccagagcgc cttgtacgcg ggcacgctca cgatcagcaa
     1261 gccgccgggc ctgagcaccc gctttagctc ggtgagggcg gcgtgctcgt catcgaggtg
     1321 ctctatcacg tccaacgccg tgatgatatc gaagtagccg tcggggaagg gcagctcggc
     1381 ggcgtcggct ttggcgaggc tggtgtgccc acgctcccgg caaaaagcca gcgcttcctc
     1441 cgagaagtcc gtgccggtgg gccgggcgta gcgggccagg tggtccaggt tcagccccgt
     1501 gccgcaaccg aggtccaggg cgtgccgctc gcggcccctg gcgggcggga ggtacctgtc
     1561 gagcaggctg tagacaatcg cccgcttgcc cacgtgccac cagtagtcct gttccttgat
     1621 gtagaggtcg tagtagaggt cttgtcgcat aagtctatcg tgtgtgatag cggtagaaaa
     1681 tcatgtcgcc aacccacgtg ttgagcaact gccacagcaa ggcggcaacc aggagcgcgg
     1741 ccagccaccg cccgatacgg cccctttccg cgaggagacc cagggtgcgg cccgccagga
     1801 gcgacacggc agccgcgacc atgtaatacc atcgtatcgc gtcacctagc gtctggtcaa
     1861 gtagtgcaaa aaccaccccg accacgagcc acgcgccgag ccagcccgag gcgaccctgt
     1921 tgagcctgag tcccacgacc gcagccgtcg cagcggcaag cacctgcgcg gggccgacga
     1981 ggtaagccac tttgcgagac agcttgtcga gggagaacgc ctggggagta gccgccccct
     2041 cggtggcctt tgccccggtg agccgcccca gcagcccgcc gaagtggttg gcgaccaggt
     2101 cgagcacgtt gcggtagtag gtgacgaaac cgaccgccac ggagaaggaa atgaggcgaa
     2161 tgagccgcca gccccggtcg gagccccagc caagccacag gcccgccacc atccagagca
     2221 ggaacaaccc ggctatggcg agagcaccta tcatcatacc gtagtgcccc aggagcgtca
     2281 agaaaaagca ggcggccaca ggcgcgcgca cccaggcgtg tcgcctgctc ccctcctccc
     2341 gcacggacag gtagaccagg ccgccgagta tgctgaggtt gagcgtgctt tgggcgaaca
     2401 ggttcgtgta gttcccggcc gagaacaggt cgaacggcgc gaccgatacg gcgtacagca
     2461 gcgcggccca ggccccactc gcgccccccc acagcctgta tccgcaccac gccagtgcca
     2521 ggcacgtcag cgaatcgagg agcgagcaca tccacttcag ggccagcgat atggcctcat
     2581 cgccgctccc aaatacccag gtgaaagggg taacgagcac gtagagggcc ggtgggtatg
     2641 gggcgggagt gccgtcgggc agcggctcgc tcatgatcca ctcgcccccg agcagcgcct
     2701 gcacgttgtg cacgttgagc agcaggtcgc tcgtgaggaa ctgcgggtag gtgaggcccc
     2761 cgaagcggag cgcgaaggcc agcacgaaag ccgccgtgcc cagtccaatc gtcagccggg
     2821 acgcgcgcgt ggagctacct gtaagcagac ccagccccaa cctccccacc actgcgaagc
     2881 ccaggcacca ggcccccatc ggcagaatcg aaggcagcag caacccgagt tcgggccggg
     2941 ctaccaggat gagcaccacc gtcgcgaacg agagcgcacc gccaacgacc agcgcgggca
     3001 acgtcctgcc ggtcgcaaac gccgccgatg cgccaaacaa cagcagcccc agcgcgagag
     3061 ccagcagagc cccaggcgag ggcagcacca ccgggcgcag gccgtactcc gcgggcgcta
     3121 cctccacact gtcgatgttg acgcccaggg cgcgtccggg gttctccgct gtgccctcgt
     3181 tgaaggtatc cacgtccatc acgacgttga ggctgccctg catgaagtcg ccacggggca
     3241 cgaagaaggt gtcggtgcgc agctcagggg gacttggcat ggcgaattgc tggccgcgta
     3301 tcgttacagt caccaccttt gccgccaggt cgttagccgc gcccacgtgg gtgatggaca
     3361 cgttgacagg ctcgttgcct atctccggga agttcactac cgctctagaa gtgctccagc
     3421 ggtactcgaa ccccggcgtc gcctccttgg catggaagcc actgacgtag gcgtc
//
LOCUS       DASRWZ010000719         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1292285, whole genome shotgun sequence.
ACCESSION   DASRWZ010000719 DASRWZ010000000
VERSION     DASRWZ010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292285"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1402)
                     /gene="eno"
                     /locus_tag="VFH60_11330"
     CDS             complement(107..1402)
                     /gene="eno"
                     /locus_tag="VFH60_11330"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET6314420.1"
                     /translation="MYEVEIKDVRAYEILDSRGNPTIEVEVSLIGGAIGRAAVPSGAS
                     TGAHEAVELRDGDKRRYGGKGVLKAVENVNVAMAEQIIGMSACDQVALDRALLALDGT
                     PNKGRLGANAILGVSLAAAHAAARHFNMPLYRYLGGLAARTLPVPMFNILNGGKHALD
                     STDFQEFMVMPTGAPNFREGLRWGVEIYQSLKKTLHDKGYSTNIGDEGGFAPSLKSNA
                     EAVELILSAVEQAGYTPGKDVHIALDPAMTELYENGSYHLTKEGRTLSSREMVAFWED
                     WTGKYPIISLEDGLAEDDWDGWRLLNERIGSRVQLVGDDLLVTNTERIERAIEAGACN
                     SLLTKVNQIGSLTEAIQAVRDAQQAGWTAVMSHRSGETEDTTIADLAVALNCGQIKTG
                     ASARGERTAKYNRLLRIEAELGESSVYPGISAFYNIRKS"
BASE COUNT          264 a          498 c          521 g          346 t
ORIGIN      
        1 aaaagaaaaa tcccacgggt actttcttaa gcaggatttt tcttttcgcg cccgtgtcac
       61 gcgctctcct gcatgagtgg ggctggcgta ttggcggctc gtggccctac gatttgcgga
      121 tgttgtagaa ggcgctgatg ccggggtaga cggagctttc gccaagctcg gcttcgatgc
      181 gcaggaggcg gttgtacttg gcggtgcgct cgccgcgggc cgaggcaccc gtcttgatct
      241 ggccgcagtt gagggccacg gcgaggtcgg ctatcgtggt gtcttcggtc tcgccggagc
      301 ggtgggacat gacggcggtc cagcctgctt gctgggcgtc tcgcacggcc tgtatcgcct
      361 cggtgaggct gcctatctgg ttcaccttgg tgagtaggga gttgcaggcc cccgcctcga
      421 tagcgcgctc gatgcgctcg gtgttggtca ccagcaggtc gtcgcccacg agctgcacgc
      481 ggctgcctat gcgctcgttc aggagccgcc acccgtccca gtcgtcctcg gccagcccgt
      541 cttccaggga gatgatcggg tacttgccgg tccagtcctc ccaaaacgcg accatctccc
      601 ggctggagag ggtgcggccc tctttcgtca ggtggtacga cccgttctcg tatagctccg
      661 tcatggcggg gtcgagggcg atgtgcacgt ccttgcccgg cgtgtaaccg gcctgctcca
      721 ccgcggagag gattagctca accgcctccg cgttgctctt gagagatggc gcgaagccgc
      781 cctcgtcgcc gatgttggta ctgtagccct tgtcgtggag ggtcttcttg agcgactggt
      841 agatctccac gccccagcgc aacccctcgc ggaagttggg cgcgcctgtg ggcataacca
      901 tgaactcctg gaagtcggtg gagtccagtg cgtgcttgcc gccgttgagg atgttgaaca
      961 tcggcacggg cagggtgcgg gcggcgaggc cacccaggta gcggtagagc ggcatgttga
     1021 agtgcctggc ggcggcatgc gcggcggcta gggacacccc aagtatggcg ttggcgccga
     1081 gacggccctt attaggcgtg ccgtcgagcg caagcagcgc ccggtcgagg gccacctggt
     1141 cgcaggcact catgcctatg atctgctcgg ccatcgccac gttgacgttc tcgaccgcct
     1201 tgagcacgcc cttgcccccg tagcgccgct tgtcgccatc ccgcagttcg accgcctcgt
     1261 gcgccccggt ggacgcccct gagggcaccg ccgcacgccc tatggcccca cctatcaggc
     1321 tgacctccac ctcaatcgtc gggttccccc tggaatcgag tatctcgtac gctcgtacgt
     1381 cctttatctc gacttcgtac acagcaagtc ctccctatgg gtaattacga attacgaatt
     1441 acggtaaagc gtaacacgta aagcttaaga cgtaaaggtg gaggtagtaa catataaagc
     1501 ggccccaagg actgtgcctc gtaagccgct ttataagtta tgaattatcg taattcgtat
     1561 ttcgtaattc ccgcctactg gcggggaatg ggcatgcgca ggaacggttc ggtgggggtg
     1621 cgctggccc
//
LOCUS       DASRWZ010000720         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_607198, whole genome shotgun sequence.
ACCESSION   DASRWZ010000720 DASRWZ010000000
VERSION     DASRWZ010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607198"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="VFH60_11335"
     CDS             <1..1454
                     /locus_tag="VFH60_11335"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET6314421.1"
                     /translation="EVNAAPGLRMHVYPSEGKSRPVGDAIVSMLFPEGTSARVPLIAI
                     TGTNGKTTVTRMIGHIYSTMKLYVGMTTTDGIYFGGVRRVKGDSAGPRSAESVLQHPM
                     VDVAVLETARGGILRSGLAWDRSTVSVCLNIANDHLGLEGIETPERLARVKRVVIESA
                     SKDGYGVLNADDPLVVDMAQDCPGSVVFFSMDPSTPALTLHLAGGGKAAYHNNGDLIL
                     AEGSKETFLMKATDIPASFGGLIPFQIQNALAAATACWVAGVPIESIRLGLRTFKADD
                     NSAPGRINMFNVGRARVIIDYGHNPHALKAVQAAIGQMKPRRSIGVVAAPGDRRDADI
                     QELAVVAANTFDWIIVREDNDLRGRERGEVAHLISETIKRTRPSLPVTIIEDEAESVE
                     QALEMARAGDLVVVFVDRVDETIEQIKRASKAVEIEQSDAFWCPVPEPSTANQRTRAE
                     AIANTTGLDDEDVLRNLDGQIKPRVAVSVKRET"
     gene            1649..2545
                     /locus_tag="VFH60_11340"
     CDS             1649..2545
                     /locus_tag="VFH60_11340"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HET6314422.1"
                     /translation="MIRIPYSGAGSALLTGPWRTPGYLMVIGGADRLDPDARLARLFI
                     QLAERVTAHSGQRDIVLVSTATRHPEVLTAEYVRIFTRLGVPRGHIHAPFIRNRDEAQ
                     EFANIRLLSEAAGIFITGGDQYALTQALDRSPAEEAIRLAYQRGAVVAGPSAGATAMG
                     RPMVVAGGGSGELRMGMVQMSHGLGWVGDDMIIDTHFGARGRFPRLASAVAEHPNALG
                     IGIDENTCLLVDGLKHCIVAGSGVVYFLDAGRIEANSASAAHSGQPISIGPVNVTMLA
                     DGGEYDLRARRILLAAGDATKV"
     gene            2571..2646
                     /locus_tag="VFH60_11345"
     tRNA            2571..2646
                     /locus_tag="VFH60_11345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2604..2606,aa:Arg,seq:cct)
BASE COUNT          614 a          933 c          889 g          477 t
ORIGIN      
        1 tagaggtgaa cgccgccccc ggcctgcgca tgcacgtcta tccatcggag ggtaagtccc
       61 gcccggtggg tgacgccata gtgagcatgc tcttcccgga gggcacctcg gcccgcgtgc
      121 cactgatcgc catcaccggc accaacggca agaccaccgt cacccgcatg ataggccaca
      181 tttattccac gatgaagcta tacgtcggca tgaccaccac cgacggcatc tacttcgggg
      241 gagtgcgccg cgtgaagggc gactcggcag gcccgcgcag cgccgagtcg gtcttgcagc
      301 acccgatggt ggacgtagcc gtgcttgaga cggctcgcgg cggcatcctg cgctccggcc
      361 tcgcgtggga caggtccacc gtgagcgtct gcctcaatat cgctaacgac cacctcggcc
      421 tcgaaggcat agagaccccc gaaaggctcg cccgcgtgaa gcgcgtggtc atagagagcg
      481 cgagcaagga cggctacggc gtgctgaacg ccgacgaccc gctggttgtg gatatggccc
      541 aggattgccc cggcagcgtg gtattcttct cgatggaccc ctccacgcct gctctcacgc
      601 tccacctggc gggcggcggc aaggcagcct accacaacaa tggcgacctc atactggcgg
      661 agggttccaa agagacattc ttgatgaaag ccaccgacat acccgcaagc tttggcggcc
      721 tgatcccctt ccagatacag aacgccctcg ccgcagcgac cgcctgttgg gtggcgggcg
      781 tgcccatcga atcgatcagg ttgggcttgc gtaccttcaa ggccgacgac aatagcgccc
      841 ctggacgcat caatatgttc aacgtgggcc gcgcacgcgt aatcattgac tacggccaca
      901 acccgcacgc cctcaaagcg gtgcaggccg ccatcggcca gatgaagcca agacgctcca
      961 taggcgtggt ggccgctcca ggagaccgcc gggacgccga catacaggaa cttgcggtgg
     1021 tagcggccaa taccttcgac tggataatcg tgcgcgagga caacgacctg cgcgggcgcg
     1081 agcgtggaga ggtagcccac ctcatctcgg agaccatcaa gcgcacccgc ccctccctac
     1141 ccgtgaccat catcgaggac gaagccgagt cggtagagca agcccttgag atggcccgcg
     1201 caggcgacct ggtcgttgtg ttcgtggacc gggtggacga gacgatagag caaatcaagc
     1261 gcgccagcaa agccgtcgag atagagcaaa gcgacgcctt ctggtgtccc gtgccggagc
     1321 catccaccgc gaaccagcgc acccgcgccg aagccatagc caataccacc ggcctggacg
     1381 atgaagacgt gctacgcaac ctcgacgggc aaatcaagcc gcgtgtggcg gtgagcgtga
     1441 aacgtgaaac gtgaaacgta ttgcgtagta cgtattgcgt aaatgttgaa ccacaaagaa
     1501 caccgggatg gagtgaataa aaccgcagag gcgcagagac gctgagatta tttaggcaat
     1561 acgcaatacg tttcacgttt tacgcaatac gagctagagg aacaacccat acgtgtcaag
     1621 taatggtaag caaaagactc cgcaggctat gattcgcatc ccgtattcgg gtgcggggtc
     1681 ggccctcctg accggaccct ggcggacgcc gggctacttg atggtcatcg gtggggcaga
     1741 tcgcctcgac ccggacgccc ggcttgcccg cctcttcatc cagcttgcgg agcgcgtaac
     1801 ggcccattcc gggcagcgcg acatcgtact ggtgagcacg gccacccgcc accccgaagt
     1861 gctcaccgcc gagtacgtgc gtatatttac gcgcctgggc gtgccgcggg gccacatcca
     1921 cgcacccttc atacgcaacc gcgacgaggc ccaggagttt gccaacatac ggctactctc
     1981 ggaagcggcg ggcatcttca tcacgggggg cgaccagtac gcgctcaccc aggccctgga
     2041 ccgctcgcca gccgaggaag ctatcaggct ggcctaccag cgcggcgcgg tagtggcggg
     2101 ccccagtgcg ggggcgaccg ctatgggcag gccaatggtc gtagcgggcg gcgggagcgg
     2161 cgagctacgc atgggcatgg tgcagatgtc gcacggcctc ggctgggtgg gcgacgacat
     2221 gattattgac acccacttcg gcgcgcgtgg ccgcttcccc aggctggctt cggcggtggc
     2281 cgagcaccct aacgccctgg gcatcggcat agatgagaac acctgcctgc tggtggacgg
     2341 gctgaagcat tgcatcgtcg cggggagcgg cgtggtctac ttcctggacg ccgggcgtat
     2401 cgaagccaac tccgcgagcg ccgcgcactc cggccagcct atctcaatcg gcccggtgaa
     2461 cgtcaccatg ctcgctgacg gcggcgagta cgaccttcgc gcccgccgca tcttacttgc
     2521 ggcaggcgac gcaactaagg tataattgac tgcgcgaaaa tggcgcatct gcgcgtgtag
     2581 ctcaggggac agagcgaggg cgtcctaagc cctgcgtcgg gagttcgaat ctctccacgc
     2641 gcaccaacgt aacgagtgcg gaaagcggaa tgcggcatgc tgaccagcga aagtagcgtg
     2701 cctgcatatt gctgatggcc taccgattgc taacagagta gcaatcgtaa cattccgcac
     2761 tccgcattcc gcattccgca ctctaaaatc tcccctggag gacactcaat ggatgtaacc
     2821 atcaggccaa agaacttcaa gctggccggt gacatcgagc aacagatccg caagcgcata
     2881 gaccgcctct ctcgccatat tgacaacatc act
//
LOCUS       DASRWZ010000721         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_58051,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000721 DASRWZ010000000
VERSION     DASRWZ010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58051"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFH60_11350"
     CDS             complement(<1..405)
                     /locus_tag="VFH60_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET6314423.1"
                     /translation="MTARDIDPHPQTQTALTDGRPHTPVRNAIVSAAVVVGLGLAIFF
                     TWRALGSPGPSPSGAPQVRARVGTIAPDFELKDINTGQMVKLSELRGRPVWVNFWATW
                     CPPCAEEMPEMQKLYDQYRHSDLALLGVDVQES"
     gene            complement(426..1859)
                     /locus_tag="VFH60_11355"
     CDS             complement(426..1859)
                     /locus_tag="VFH60_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET6314424.1"
                     /translation="MNNIPWIIYGAVVLGVALVVGRHMGRFREAYTEMTGMMVGMGMG
                     MLNGFLLGYAAAAATSSMFWGNLIGMPLGVLLGAWYGRAGSLMGVLDGGMGGGMGGSM
                     GAMLAAMLAFPAWTMNVTGLLLVALYIPGMLGLVLLIERSAPGHAAMHRLLPFFTRAI
                     STEAHENLANGATHAHHAPRITDYYAFLGISPDADADRIAQAYLEKLAVSSPADVRTA
                     EVAFATLTDPHRKRAYDAVLAANRPAADKGDNYPPRRKKQAAQPVASTAAPAANNNAA
                     TARVKSRGSRQQTQQVHRSVQERPRAVERHKKEAPISWVGVTAGIVTLAVLGGWWFAS
                     QSGMLAGAGSQATGGLGTPVLGYVNNERNLPETEVRKLEATAVTVPVAADGKQNVDLT
                     VIGDTMSYKPRVLKVKKGVPVHFNISVQGRDPGCGRFVAFRGLGAHGLAEPGQVTTAE
                     FTASDTGVYEINCGMEMMEPGYILVTE"
     gene            complement(2168..>3709)
                     /gene="lon"
                     /locus_tag="VFH60_11360"
     CDS             complement(2168..>3709)
                     /gene="lon"
                     /locus_tag="VFH60_11360"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6314425.1"
                     /translation="ALGRRFVRVSLGGIRDEAEIRGHRRTYIGALPGRIIQTMKTAAT
                     VNPVFMLDEIDKLGADFRGDPSAALLEVLDPEQNNSFSDHYLDVPYNLSKVLFIMTAN
                     MLDPIPPALLDRMEVIELPGYIEDEKYHIARQFLVPRQIDEHGLTAAHLKISDGALRR
                     LIREYTHEAGVRNLEREIGGICRKVAKKVAELMPVTQDETPAEAAVMDKPLPILSTDQ
                     ATEPLGHLNPDDLRAASAAIEAADAISQDDVTDMAPPVGEQTADGQKRPRARVIHERD
                     LEEYLGPARYSYGLAEEQDEVGVATGVYWSPMGGDTIGVEVTLMEGRGTLMLTGQLGD
                     VMKESAQAALSYARSRAPQLGIDPARFEKTDIHIHVPAGAVPKDGPSAGVTLATALVS
                     ALTGRKVRRDVAMTGEITLRGKVLPIGGLKEKMLAAHRAGISTFVLPAKNKKDLAEIP
                     GKVLRQIQVVPADDLDKVLDVALRPAPNLSDPAEPTPDAQSKATLRPKAPSHRRTGIS
                     GIPAS"
BASE COUNT          585 a         1128 c         1283 g          713 t
ORIGIN      
        1 gctttcctgc acgtccactc cgagcagggc gaggtcgctg tggcggtact ggtcgtacag
       61 cttctgcatt tcgggcatct cttcggcgca gggcgggcac caggtagccc agaagttgac
      121 ccacaccggc ctgccccgca gctcggatag cttcaccatc tgccccgtgt tgatgtcctt
      181 cagctcgaag tcgggggcga ttgtgccgac ccgcgccctc acctgtgggg caccggaagg
      241 ggatggacca gggctgccca aagctcgcca ggtgaagaag atggccaggc cgaggcccac
      301 gaccacggca gcactcacta tggcgttgcg cacgggggtg tgaggtctgc cgtcggttag
      361 tgccgtttgc gtctgggggt gagggtctat gtctcgtgcc gtcatgcgct ccacgcgtgc
      421 ttgctctact cggtgacgag tatgtagccc ggctccatca tctccatgcc gcagttgatt
      481 tcgtacacac ccgtgtctga ggcggtgaac tctgcggtgg tgacctgtcc cggctccgcg
      541 aggccgtggg cacccagccc tcggaaggcc acgaagcgcc cgcagccggg gtcgcgaccc
      601 tgcacgctga tgttgaagtg gaccggcacg cccttcttca ccttgagcac gcggggcttg
      661 tacgacatcg tgtcgccaat tacggtgaga tcgacgttct gcttgccgtc cgcggccacc
      721 ggcacggtta cggccgtcgc ttcgagcttg cgtacctcgg tttcgggcag gttgcgctcg
      781 ttgttcacgt agccgagcac cggggtgccc aggccgcctg tcgcctggct accggctccc
      841 gccagcatgc ccgattgaga ggcaaaccac caaccgccca gcaccgccaa agtgactatg
      901 cctgcggtga cgcccaccca ggagatagga gcctccttct tatgccgctc gacagccctg
      961 ggccgctcct ggacgctcct atgcacttgc tgtgtctgct gtctcgaccc gcgagacttc
     1021 acccgggcgg tggccgcatt gttgttggcg gcgggcgcgg ccgtagaagc tacgggttgt
     1081 gctgcctgct tctttcggcg gggcgggtag ttgtcccctt tgtcagccgc agggcggttg
     1141 gcggccagca ccgcatcgta ggcacgcttc ctgtgtgggt cggtgagggt ggcgaaggct
     1201 acctcagccg tgcgcacgtc agcgggactc gagacggcca gtttttcgag gtaggcctgc
     1261 gctatgcggt cggcgtccgc gtcgggtgag atgcccagga aggcgtagta gtcggtgatg
     1321 cgaggggcgt ggtgggcgtg agtggcgccg tttgcaagat tctcgtgcgc ttcggtgcta
     1381 atggcgcggg tgaagaaggg gagtaggcgg tgcatagcgg cgtggccggg cgcgctcctc
     1441 tcgatcaaga gcaccaggcc cagcatgccc ggtatgtaga gagcgacgag cagcaggccg
     1501 gtcacgttca tagtccaggc ggggaaagcg agcatggcgg caagcatcgc gcccatcgag
     1561 ccgcccatcc ccccgcccat accgccatcg aggacgccca tgagggagcc tgcccgcccg
     1621 taccacgcgc ccaggagcac gcccagaggc atgcctatca ggttgcccca gaacatgctt
     1681 gaggtagcgg ccgcggccgc gtaaccgagg aggaagccgt tgagcatgcc catgcccatg
     1741 cccaccatca tgcctgtcat ctcggtgtag gcttcccgga agcggcccat gtggcggccc
     1801 acaacgagcg ccacacccag cacgacggct ccgtagatta tccagggaat gttattcatg
     1861 tagctatcct gactgtacgg ttatagaacg ctagcgcgag tccttactat tatacgatgt
     1921 tcgaggtata gcatcaggta agcattggct gaaaagaggt aaaaaggcgg ctaacttgca
     1981 acgatggggc gatagacgtg ggaggagaaa tgaaaccgca aaggcgcaaa gagcgcaaag
     2041 gacactagag aatgaaggtt agcgggtaca aagaggacca gggtactaca tcctgtactc
     2101 tggtccttaa gcttataacc ccctgcttta tcgttccgtg tcctttgcgc tctttgcggt
     2161 ttcacgtcta actggccggt atgccgctga tgcccgtcct gcggtgggag ggggctttag
     2221 ggcggagggt ggccttggat tgggcgtcgg gggtcggctc ggcggggtcg ctcaggttgg
     2281 gggcggggcg cagggccacg tccagcacct tgtcgaggtc gtcggcgggc accacctgta
     2341 tctggcggag caccttgccg ggtatctcgg ccaggtcctt cttgttcttg gcgggcagca
     2401 cgaaggtgga tataccggcg cggtgggcgg ccagcatctt ctccttgagg ccgccgatgg
     2461 gcagcacctt gcctcggagg gtgatctcgc ccgtcatcgc cacgtcgcgg cgcaccttgc
     2521 gtcccgtgag ggccgatacc agcgcggtag ccagggtgac gcccgccgag gggccgtcct
     2581 tgggcacagc gcccgcgggc acgtggatgt ggatgtcggt cttttcgaag cgggccgggt
     2641 cgatgccgag ttgcggggcg cggctgcggg cgtaggagag ggccgcctgg gccgactcct
     2701 tcatcacgtc gcctaactgc ccggtgagca tcaacgtgcc ccggccctcc atgagggtca
     2761 cttccacccc tatcgtgtcg ccacccatcg ggctccaata gacgcccgtg gccacgccga
     2821 cctcgtcctg ctcctccgcc agaccgtagc tgtagcgggc ggggccgagg tactcttcga
     2881 ggtcgcgctc gtggattacg cgggcgcgag ggcgcttctg gccgtcggct gtctgctcgc
     2941 caacgggcgg ggccatgtcg gtcacgtcgt cctgggagat agcgtcggcg gcttcgatag
     3001 cggcagaggc ggcgcgcagg tcgtcggggt tgaggtggcc cagcggctcg gtggcctggt
     3061 cggtggagag gataggtagg ggcttgtcca tcacggcggc ctcggcgggg gtctcgtcct
     3121 gggtgacggg catcagctcg gccaccttct tggctacctt gcggcagata ccgccgattt
     3181 cgcgctccag gttgcgcacg cccgcctcgt gggtgtactc gcggatgagg cggcgcaaag
     3241 ccccgtcgct gatcttgagg tgcgcggcgg tgaggccgtg ctcgtcaatc tggcggggca
     3301 ccaggaactg ccgggcgatg tgatacttct cgtcctcgat gtagcccggc aactctatga
     3361 cttccatgcg gtcgagcagg gcggggggta tagggtcgag catgttggcg gtcatgatga
     3421 acaggacctt ggacaggttg tagggcacgt cgaggtagtg gtcggagaag gagttgttct
     3481 gctccgggtc tagcacctcg aggagggccg ccgaggggtc gccccggaag tccgcgccga
     3541 gcttgtctat ctcgtcaagc ataaataccg ggttgaccgt ggccgccgtc ttcatcgtct
     3601 gtatgatgcg gcctgggagc gcgcctatgt aggtgcggcg gtggccgcgt atctctgcct
     3661 cgtcgcggat gccgccaagc gacacgcgca cgaacctgcg gcccaatgc
//
LOCUS       DASRWZ010000722         6623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1155042, whole genome shotgun sequence.
ACCESSION   DASRWZ010000722 DASRWZ010000000
VERSION     DASRWZ010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155042"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /gene="prfA"
                     /locus_tag="VFH60_11365"
     CDS             <1..820
                     /gene="prfA"
                     /locus_tag="VFH60_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET6314426.1"
                     /translation="LEAQLNEIKRLLLPKDPLDDKNVIIEIRGGAGGEEANLFAHELY
                     GMYTRYADKQRWKTEILSLNPSEVGGVREVIFEVKGQGAYSHFKYESGTHRVQRVPVT
                     ESQGRIHTSTATVAVLPEAEDVDVEIKDDDLKIDVYRSGGHGGQSVNTTDSAVRITHI
                     PSGIVVTCQDERSQLKNRIKAMTVLRSRLYEMELARRQQELGDERRSQVGTGDRSEKI
                     RTYNFPDDRVTDHRIKMTVSNVPRVLQGDIDNIIETLRVTDQAERLRAAGLASE"
     gene            831..1676
                     /gene="prmC"
                     /locus_tag="VFH60_11370"
     CDS             831..1676
                     /gene="prmC"
                     /locus_tag="VFH60_11370"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET6314427.1"
                     /translation="MTIMTIGEALLQARKRLSPNSSTPQLDAEVLLAHVLGTGRAALL
                     ARTGDPLAPEQARAFEDLAARRTRGEPVAYITGHKEFYGLDLLITRDVLVPRPETESV
                     VEACLEALPKGEVSQLADIGTGSGAILVAVLANRPQTTGFGTEISPEALEVARQNCLR
                     HGVADRATLLLGDLLEPLTNPVNVIAANLPYVSHGEASPDVATWEPNVAVFGGGEDGT
                     ATIRRFLHAAPDYLLPGGTVVMETAYSQGKAVAELAKAAFPGASVEVRKDLAGYDRIV
                     VVKTR"
     gene            1673..2503
                     /locus_tag="VFH60_11375"
     CDS             1673..2503
                     /locus_tag="VFH60_11375"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET6314428.1"
                     /translation="MTVKLLALDLDGTLFTDDLVISVRTREAIRATQARGVLVTLATG
                     RMFTSARKIAGDLGIDAPLICYQGALVQHSGTGEVLYHKTVPLALTHEIITATASRGL
                     HLNVYLDDRLYVAHMTEGALFYSQINYGLELNQVGDLHSWLDAQGGREPTKLVIVTPQ
                     EQTDAVLAEFTAEYGERLQVTKSHPRFTEFTNIECSKGRALAFLAAQVGVGREEVMAI
                     GDGHNDLDMLAWAGYGLAMPTAPQAVLDVAHSICLPLHADGAAEAIERLILALGQPGK
                     "
     gene            2574..4433
                     /locus_tag="VFH60_11380"
     CDS             2574..4433
                     /locus_tag="VFH60_11380"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HET6314429.1"
                     /translation="MKAERTPRTTRRAAPPADDDAILDAAVEPARPERQATGAATDGR
                     PTPNGASARVENAQESESVPTVEPSAGQVAESVVAPAAADVAPGVTRIIDTTAPQATL
                     PTRGGLVSRRLPRFDMDERLGRLGLALILAVLLWFYVVNLENPAQTTSFRDLTIDVRG
                     TSSNLRVISPLPTVDVTIQAPQNLMTNLSPADVHPYIDLSTMTEGVHQIPIDIETRGL
                     PPESLTVNVEPSQVQVQLQLQITEVFSIAVQLEGTPPLGYKFDPAQVTPRSVEVTGSQ
                     DAVSRISQVLVPVDIDGQTTRVQGSRTPRAVDADGRDVTGLTFEPANVQVLVPIEAYV
                     RNKTVSVLPVVVGQPASGYRVAALTADPNNVQICCSEEVLGPIDFLSVVAVPITGTTS
                     TIVTTTQLVLQPGVDLLPGQPREITVTVEVEELQSTIELSLAPVAQGMPSGYSAFITP
                     DRVDVTLQGSFNRLQQIAPADIRVVANVSGLGAGDHEVTFQTIVPEGVTLQRISADEA
                     TVTLVAPTPVPPTATPQPSPTQTTEPTPAPTITEPVVAPVTTSTSLPVATATQPAPSR
                     TPTAVLTATPTVAPTTTVIPTSEPAGVEGEQPPALSPTPTTGSRLTVYEAAHR"
     gene            complement(4567..6420)
                     /locus_tag="VFH60_11385"
     CDS             complement(4567..6420)
                     /locus_tag="VFH60_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HET6314430.1"
                     /translation="MDVKDFGFTRDETYSEQIGQYLNPLPRSVVRPGEVQLLDGEWRF
                     ELDPEDQGLREEWFRQHSYKRKAMWPSSIEEHMAKAAEQARITGPLAQYTPDNVVAWY
                     ERDFTIPEHWLGDPERIVQITFGACGYETRVWLNGTQLSTIEGEQVHYGEYTSFSYEL
                     PPGLLRPTNRLTVRTADTLDAEITRGKQESRVYKRGGIWYQTVSGPVRSVWIEPVLLN
                     RLRSRLEVLSTIEDGLVEFIVTTRTATRGTYNLRLVVTPYNKAEQAVVSEFKLALDIG
                     VKRQRLVMEIPGAKLWSPDEPNLYGLVAQLTTPGGAISQIGTHFGLRRIEAHGRHIYL
                     NDRETYLDGILYQPGTSTYEQIANHMHAMKALGCNLVRVHIAGIDPRIYDLADEVGML
                     LWVEVPSPHVSTQRSRDNHWAELTRMLPILCSHPSVVILSLYNEDWGVQDIQFNALTR
                     EYIARTWAYLRVNYPQLLVVDNDGWRHVSTEGRVESHLLTAHIYRQELDEWRERLDLL
                     VSGENEGVAPHPLVVGDPFFYRGQVPLVVSEWGGFGFVEYGGAKDMAARVGQIEAFKH
                     ELRKRPIAGDVYTQATSIEDEVNGLIDPATGELLVPEGLLYSAPVEAVEPP"
BASE COUNT         1242 a         2232 c         2002 g         1147 t
ORIGIN      
        1 gttagaagcc caactcaacg agatcaagcg cctgctgctg cccaaagacc cgcttgacga
       61 caagaacgtt atcatcgaaa tccgtggcgg ggccgggggc gaggaagcca acctcttcgc
      121 ccacgagctg tatggcatgt acaccaggta cgccgacaag cagcgctgga agaccgagat
      181 cctgtcgctc aaccccagcg aggtgggcgg cgtgcgggaa gtcatattcg aggtaaaggg
      241 ccagggtgcc tacagccact tcaagtacga gagcggcacg caccgggtgc aacgcgtgcc
      301 cgtgaccgag tcgcagggcc gcatccatac ctccaccgcc accgtggccg tgctgccgga
      361 ggcggaggac gtggacgtag agatcaagga cgacgacctc aagattgacg tgtatcgctc
      421 cggcgggcac ggcgggcaga gcgtgaacac caccgactcg gcggtgcgca tcacgcacat
      481 tccgagcggc attgtggtga cctgccagga cgagcgcagc cagctaaaga accgcatcaa
      541 ggcaatgacc gtgctacgca gccgcctgta cgagatggag cttgcccggc ggcaacagga
      601 gctgggcgac gagcgtcggt cgcaggtggg cacgggcgac cgctccgaga agatacgcac
      661 ctacaacttc cccgacgacc gcgtgaccga ccaccgcatc aagatgaccg tctcgaacgt
      721 gccccgcgtg ctacagggcg acatcgacaa catcatcgag acgttgagag tgaccgacca
      781 ggctgagcgg ctgcgtgccg ccgggctggc gtccgagtaa cccgccgtcc atgacgatca
      841 tgaccattgg agaggcgctt cttcaggcga ggaagcgcct ctcgcctaat tcctccactc
      901 cccagctcga cgccgaggtg ttgctggctc acgtgctggg caccggccgg gccgccctgc
      961 ttgcacgcac cggcgacccc cttgcaccgg agcaggcgcg cgctttcgag gatctggctg
     1021 cgcgcaggac caggggcgag ccggtggcct acatcacggg gcacaaggag ttctacgggc
     1081 tggacctgct aatcaccagg gacgtactcg tgccgaggcc cgaaaccgag agcgtggttg
     1141 aggcttgctt ggaggcattg ccaaagggtg aagtaagcca attggctgat ataggcacag
     1201 gaagtggggc tattctggtt gcggtgctgg ctaacaggcc ccaaacaact ggattcggca
     1261 ccgaaatctc gcctgaagcc ctggaagtag ccaggcagaa ctgcctccgg catggggtcg
     1321 cggatcgcgc tactctgctg ctgggcgacc tgctggagcc tttgaccaac cccgtgaatg
     1381 tgattgccgc caacctgccc tacgtgtcgc acggcgaagc ctcccccgat gtggctacct
     1441 gggagccaaa cgtagcggtg tttggcggcg gcgaggacgg cacggccacg ataaggcgct
     1501 ttctgcacgc ggcccccgat taccttctcc ccggcggcac tgtagtgatg gaaacagctt
     1561 actctcaggg taaagccgtc gcagagctgg caaaggctgc gtttcccggc gcgtcggtcg
     1621 aagtgcgcaa ggacctggcg ggctacgaca ggatcgtggt ggtaaagacc cgatgaccgt
     1681 caagttgctc gccctcgacc tggacggtac cctcttcacc gacgacctgg ttatatcggt
     1741 gcgcacccgc gaggccatca gggcgacgca ggcgcgcggc gtgctggtga cgctggccac
     1801 ggggcgcatg ttcacctcgg cccgcaagat agcaggcgac ctcggcatag acgccccgct
     1861 gatttgctac cagggtgccc ttgtacagca cagcggcacc ggggaggtgc tgtaccacaa
     1921 gacggtgccc ctagccctca cacatgagat tatcaccgcg actgcaagcc ggggcctgca
     1981 cctcaacgtc tacctggacg accggctcta cgtggcccac atgacggagg gtgccctctt
     2041 ctactcgcaa atcaactacg gcctcgagct taaccaggtg ggtgacctgc actcatggct
     2101 cgacgcgcag ggaggccggg aaccgaccaa gctggtgatc gtcaccccgc aagagcaaac
     2161 ggacgccgta cttgcggagt tcaccgcgga gtatggagag cggcttcaag tcacgaagtc
     2221 gcacccacgc ttcaccgagt tcaccaacat agagtgttcc aagggcaggg cactggcctt
     2281 cctggcggca caagtgggcg tagggcgcga ggaggtcatg gcgattggcg acggccacaa
     2341 cgacctcgac atgctcgcct gggcgggcta cggcctcgcg atgcctaccg ctccccaggc
     2401 cgtcctcgac gtagcccaca gtatctgcct ccccctccac gcggacggag cagccgaagc
     2461 catcgagcgc ttgattctcg cgttgggcca accaggaaaa tagccggttt catgcctttc
     2521 gaggtcggat tttactgtcc tacccccggc tggtgcgtta taatgtctga agcatgaagg
     2581 ccgagcgaac gccccgaaca acccgtagag cagcgccccc cgcagacgac gatgccatcc
     2641 tggatgcagc ggtggaaccg gcgcgcccgg aacgccaagc aaccggcgct gccaccgacg
     2701 gtcgcccgac cccaaatggg gcttccgcac gtgtggaaaa cgcccaggaa agcgagtccg
     2761 tccccaccgt cgagccttct gccggacagg tggccgagag tgtagtagcg cccgctgctg
     2821 ccgatgttgc ccccggcgtc actaggataa tcgataccac tgcgccccag gccacgctcc
     2881 ccacgagagg gggcctcgtg agcaggcggt tgccccggtt tgacatggac gagaggctcg
     2941 gacgcctcgg cctggccctc atacttgcgg tgctgctctg gttctacgtc gtaaacctgg
     3001 agaaccccgc ccagaccacc tcctttcgcg acctcaccat cgacgtccgc ggcacctcct
     3061 ccaatctgcg cgtcatcagc cccctgccca ccgtggacgt gaccatccag gccccccaaa
     3121 acctcatgac caacctgagt ccggcggacg tccaccccta catcgacctg tccaccatga
     3181 ccgagggcgt gcaccagatt cctattgaca tcgagacgcg cggcttgccg ccggagagcc
     3241 tcacggtgaa cgtcgagccg agtcaggtgc aggtccagct tcagctgcaa ataacagagg
     3301 tcttttcgat agcggtgcag cttgagggca ctccgcccct gggctacaag ttcgaccccg
     3361 cccaggtgac cccgcgcagc gtcgaggtca caggctccca ggacgccgtg tcccgcatca
     3421 gccaggtgct cgtgcccgtg gacatcgacg gccagaccac gcgcgtgcag gggagcagga
     3481 cgcccagggc ggtcgatgca gacgggcgcg acgtgacggg cctcacgttc gagcctgcaa
     3541 atgtgcaggt gctcgtccca atagaggcct acgtgcgcaa caagacggtg tcggtgctgc
     3601 ctgtagtcgt ggggcaaccc gcctccggct accgcgtggc cgcccttacc gccgacccca
     3661 acaacgtgca gatatgttgc agcgaggagg tgctcggccc catcgatttc ctcagcgtcg
     3721 ttgccgtgcc catcacgggg accacttcca ccatcgttac gaccacacaa ctggtcttgc
     3781 agcctggtgt ggacctcctg ccgggccagc cgcgcgaaat tactgtcacg gtagaggtgg
     3841 aggagttaca gagcaccatt gagttgtcgc tcgccccggt ggcgcagggc atgccctcag
     3901 gctactcggc cttcatcaca ccggaccgcg tggacgtgac cctccagggt tcgttcaacc
     3961 gcctgcaaca gattgccccg gcggacatac gcgtagtggc taatgtctcc ggcctcgggg
     4021 cgggcgatca cgaggttaca ttccagacga tagtgccgga aggcgtcacg ctgcaacgca
     4081 tctcggcgga cgaggcgacc gttacgctgg tcgcacccac cccggtgccg ccaaccgcca
     4141 ccccccagcc ttcgccaacc cagacgacgg agccgacccc cgcgcccacc atcacggaac
     4201 ccgtagtggc ccccgtcacc acctcgacgt cgctgccggt cgccacggcc acgcagcccg
     4261 ctccaagtcg aacccccacg gccgtactca ccgccacgcc caccgttgcc cccaccacga
     4321 ctgtgatccc tacatctgaa cccgcaggcg ttgagggtga acaaccaccc gcactttcgc
     4381 ctacccccac gactggctcc cgcttgaccg tctacgaggc cgcgcaccgc tagcaatgcc
     4441 ctggcagata aatctgcggg ctgccgttac aagtccccct acggggacta cccagtagat
     4501 gaggtagtct ccgtaggggg actttgtagt tgttgctcgc ggtttcaacc gccgggcagc
     4561 tagggcctac ggcggctcaa ccgcctcgac cggcgcggag taaagcagcc cttccggcac
     4621 gagcaactcc ccggtcgccg ggtctatcag gccgttgacc tcgtcttcga tgctggtggc
     4681 ctgggtgtaa acgtcgccgg ctatggggcg cttgcgcagc tcgtgcttga acgcctctat
     4741 ttgccccacg cgcgccgcca tgtccttcgc gccaccgtat tcgacgaagc cgaagcctcc
     4801 ccactcgctg accaccagcg gcacctgccc ccggtagaag aacgggtcgc ccaccaccag
     4861 cgggtgcggg gccacaccct cgttttctcc gcttacgagc aggtcgagcc gctcccgcca
     4921 ctcgtctagc tcctgcctgt agatgtgcgc cgtcagcagg tgcgactcta ccctcccctc
     4981 ggtcgagacg tgtcgccagc catcgttgtc caccaccagc agttgcgggt aattcacccg
     5041 caggtaggcc cacgtcctgg cgatatactc gcgggtgagc gcgttgaact ggatgtcctg
     5101 cacgccccag tcctcgttgt acagcgagag gatgaccacc gaagggtgcg agcagaggat
     5161 gggcaacatg cgtgtaagct ccgcccagtg gttgtcgcgg ctgcgctggg tcgagacgtg
     5221 cgggctgggc acctccaccc acaacagcat gcccacctcg tcggccaggt cgtagatgcg
     5281 agggtcgata ccggcgatat gcacccgcac gaggttgcag ccgagagctt tcatggcgtg
     5341 catatggttg gcaatctgct cgtaggtgga ggtgcccggc tggtagagga tgccgtccag
     5401 gtacgtctcg cggtcgttta gatagatgtg ccgcccgtgc gcttctatgc gcctgaggcc
     5461 gaagtgggtg ccaatctggg agatcgcccc gcccggcgtg gtaagctgcg ccaccagccc
     5521 gtagaggttc ggctcgtccg gcgaccacaa tttagcgccc ggtatttcca tcaccagccg
     5581 ctgcctcttc acgccaatgt ccagggccag cttgaactcc gacacgacag cctgttcggc
     5641 cttattgtaa ggggtcacga ccaggcgcag gttgtaggtg ccccgcgtgg cggtgcgcgt
     5701 ggtgactatg aactccacca gcccatcctc aatcgtgctg aggacttcca ggcgagagcg
     5761 cagtctgttg agcaacaccg gctcgatcca gacgctccgc acggggccgc tgaccgtctg
     5821 gtaccagatg cccccccgct tgtagacgcg cgactcctgc ttgcccctgg taatctcggc
     5881 atccagggtg tcggccgtgc gcacggtaag gcggttggtg ggtcggagca gccccggcgg
     5941 caattcgtag ctgaaggagg tatattcgcc gtagtgcacc tgctcgcctt ctatcgtgct
     6001 cagttgcgtg ccattcagcc acacgcgcgt ctcatagccg cacgccccga aagtgatctg
     6061 cacgatgcgc tcagggtcgc ccagccagtg ctccgggata gtaaaatcgc gctcgtacca
     6121 ggccaccacg ttatcgggtg tgtactgggc aagtgggccg gtaatgcggg cctgctccgc
     6181 cgctttagcc atgtgttcct ctatggaaga aggccacatg gccttgcgtt tgtagctgtg
     6241 ctgcctgaac cactcctcgc gcagcccctg gtcctcgggg tccagctcga agcgccactc
     6301 gccgtcgagc aactggacct cgccgggccg caccacggag cggggtagcg ggttgaggta
     6361 ctgtcctatc tgctcggaat acgtctcgtc gcgtgtaaag ccgaagtcct taacgtccat
     6421 cttcccccct gttgccatct atgcaatgtg cccggaaaca cgagcggccc cgctagacta
     6481 ctcacctatc ctaatctgtt agacgtgaca tcaaaagcga ctttgtcgaa gaacggcact
     6541 caaaccccgc ctctgtcatt ctgagcgcag cgaagaatct cgtccgctac ccgtacgttc
     6601 cccttttctc atgagactct ttg
//
LOCUS       DASRWZ010000723         8506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_881408, whole genome shotgun sequence.
ACCESSION   DASRWZ010000723 DASRWZ010000000
VERSION     DASRWZ010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881408"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="hflX"
                     /locus_tag="VFH60_11390"
     CDS             complement(<1..677)
                     /gene="hflX"
                     /locus_tag="VFH60_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET6314431.1"
                     /translation="MEKAILVGVETGKQTLSMEDSLDELEQLAATAGVEVLGRITQKL
                     NDPNRATLVGKGKVDEIKTLREQLQADVVLFDEELTPTQQRNLEEVLGTKLLDRTGLI
                     LDIFARHAQTREGRLQVELAQLEYRLPRLTRMWTHLSRQAAGGVGLRGPGETQLEVDR
                     RRSSERIAKLRDELKEVHSHRELYRQKRRREGLPIVALVGYTNAGKSTLLNALSKSDV
                     YAANVLFA"
     gene            complement(768..1928)
                     /locus_tag="VFH60_11395"
     CDS             complement(768..1928)
                     /locus_tag="VFH60_11395"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HET6314432.1"
                     /translation="MRFAERLNKLPPYVFAGMAKRIADLRASGTEVINLTMGDPDVPT
                     PGFLLDALCEAAQKPENSRYPDYYGKKKLREAVADWYRVRFGVELEPPTEVLPLIGSK
                     EGIANVALAFVDPGEAALVPDPAYPVYKFGTIMADGVICPLPLKAENGWLPDLDALDA
                     DTSRAVKVLWLNYPNNPTGAIAGLDVFERVIAWAKRYDVIIAHDNPYSEITFDGYTAP
                     SILQVPGAKDVAVEFNSLSKTYSMAGLRIGMVVGNAEIVGALGRIKTNIDSGIYGAVQ
                     EAAYAALSGDQSWRPARNEIYRKRRDKLCHALGAIGIHVEPPKASLYLWAPVPDGYTS
                     AQFSELMLSQIGVAITSGASYGAQGEGWFRISLTVSDEEVDQAIARVPKLQF"
     gene            complement(2016..2969)
                     /gene="miaA"
                     /locus_tag="VFH60_11400"
     CDS             complement(2016..2969)
                     /gene="miaA"
                     /locus_tag="VFH60_11400"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET6314433.1"
                     /translation="MSSLLPPLVAVTGPTASGKSALALRLAEHFGGEIVTADSRQVYK
                     LMDIGTEKPSPEERARLPHYMIDLVYPDEPYTLALYQRGAYATINEVLARGNVPILAG
                     GTPLYVNAVLEGWTIPEVEPDMELRATLEAEAAAGGPGPLHRRLAELDPAAAEGILPT
                     NTRRIIRALEVIARTGRQISEQQDKVPPPYRILTIVLHCERPELYRRIDLRVDRQLER
                     GLVDEVAALHARGYGFDLPSMSGIGYRQIGEYLTGRATLEQAAQRIKWDTHNFSRHQG
                     NWFRRAKAAALIDVTDSDPFPEAQRLVTQFFEAAERVRGNP"
     gene            3152..4183
                     /gene="mltG"
                     /locus_tag="VFH60_11405"
     CDS             3152..4183
                     /gene="mltG"
                     /locus_tag="VFH60_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET6314434.1"
                     /translation="MIVVVLAAGAGIYMFVGNALQSGESGATDSVPVAFEIKPGEGVN
                     DVSSNLERQSIVDSAFWFTLQLRSSGKDKLIKAGTFQLRTNMDNSEVIEVLTAPIADE
                     PGVRFTVIEGLRLEETAEKLGAQGLITPSIFLDAVRSPEGVAKYGGAFFQEAGAPADA
                     TLEGYLFPDTYEIKQNPGDNTDAVIKIMLDTMQQRITPEMREKLTAQGRTVHQMLTVA
                     SIVQREGVVKEELSTIAAVFWNRVDAGMQLGADPTIQYAIGQSPTWWPPLNLVPNEVE
                     SPYNTYVIIGLPPGPICAPGLAAIEATVNPETTDYLYFVAKEDGTHTHAFSRTLEEHE
                     RNRALYGNK"
     gene            complement(4299..5306)
                     /gene="lhpH"
                     /locus_tag="VFH60_11410"
     CDS             complement(4299..5306)
                     /gene="lhpH"
                     /locus_tag="VFH60_11410"
                     /EC_number="4.2.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050346 - trans-L-3-hydroxyproline
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-3-hydroxy-L-proline dehydratase"
                     /protein_id="HET6314435.1"
                     /translation="MHFDRVYSTIDCHTAGEPLRIVTSGIPFIPGATILERRSWVREH
                     LDHVRRALMHEPRGHADMYGCYVTPPVTPNADLGVIFMHNEGYSTMCGHGVIALAAVA
                     VATGMVPATTPETRVGLDTPAGFVEAFVEWDGRKTGRTRFLNVPSFLYRRDLVVDTPG
                     FGRLTVDVAFGGAFYAYLEAEQADLTVRPADYRPLIQLGDEVKHAVEAAIEVVHPLEP
                     ALHGIYGTIISGPPLAPGADQANVCVFADREVDRSPTGTGTAGRVAQLHARGLLPVGR
                     EFVNESIIGTRFTARVVEETMVGDFPAVVPEVAGQGYIVGFCQWVVDADDPVAEGFFL
                     R"
     gene            complement(5680..6870)
                     /locus_tag="VFH60_11415"
     CDS             complement(5680..6870)
                     /locus_tag="VFH60_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6314436.1"
                     /translation="MFGRVVRVGLASLLLALLVVLGGLSVTGIASTLSFGPPSTAGAG
                     TGAEVKPASNDAAQPASPDNAVLAPEPPAPTQGGVLDARIAARRTGSAVVTVVNYLDT
                     NAQPGAFGAIPQASGSGVIVDARGYIVTNNHVVANQRSLEVVFSDGKKAAATLVGTDP
                     FSDLAVLKVDAKMPAVASWGDSDKLEPGQPVVAIGSALGDYQNSVTAGVVSQVHRDIK
                     DADSTALRDLIQTDAAINHGNSGGPLLDIEGNVIGINVAVVRNTGVGDVAEGLGFAIP
                     SNTARAVVDQIINNGVVSRPYIGITYQMINERLAGAENLARDHGILVSEVSPGTPADK
                     AGITPGSIITRFEGVELNEDNTLMELLMERKVGDTVTLTVLEPGATGEKDLQITLAAR
                     PKDQ"
     gene            complement(7266..7496)
                     /locus_tag="VFH60_11420"
     CDS             complement(7266..7496)
                     /locus_tag="VFH60_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314437.1"
                     /translation="MDDASGGGAGTTRPPVLDNASETQILELQRQFTEDDRQGWNALT
                     RSYGWSDQESNEVWSWFAQQPEGGAGSSGGGL"
     gene            complement(7558..7815)
                     /locus_tag="VFH60_11425"
     CDS             complement(7558..7815)
                     /locus_tag="VFH60_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314438.1"
                     /translation="MSDQITRTERTTTTSTEVVPAVPVVPVETATTTSTTTTTEVQPA
                     APQTTNINVVPTTGVETAGGGSVSVNVPGPGTGTTNVNVND"
     gene            complement(7919..8290)
                     /locus_tag="VFH60_11430"
     CDS             complement(7919..8290)
                     /locus_tag="VFH60_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HET6314439.1"
                     /translation="MQDPNDRAPETTTIDVFDRVDMRAGRITRVEPFPEARKPSYKLE
                     IDFGPLGTRKSSAQLTAHYTPEELVGKMVVAVVNFPPRRIAGFASEVLVLGLPDAGVA
                     REGGVILLAPEREVEPGARVF"
     gene            complement(8303..>8506)
                     /locus_tag="VFH60_11435"
     CDS             complement(8303..>8506)
                     /locus_tag="VFH60_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314440.1"
                     /translation="QWSLMDFAPRSTAKVANFEEHFGLFRLDGTPKPAAEIFKNGYSG
                     RILPSQTKTYLPLDTADHPNLNP"
BASE COUNT         1537 a         2599 c         2667 g         1703 t
ORIGIN      
        1 gcgaacagca cgttagcggc gtacacatcc gacttgctca gcgcgttgag cagagtagac
       61 ttgccagcgt tcgtatagcc cacgagggcc acaatcggga gaccttcgcg gcggcgcttc
      121 tgccggtaca actcgcggtg ggagtggact tccttcaatt cgtcacgcag cttggcgatg
      181 cgctccgagg agcggcggcg gtccacttca agctgcgtct cgccaggacc gcgcagaccg
      241 accccgccag ccgcctgccg ggaaaggtgg gtccacatgc gggtgaggcg gggcagccgg
      301 tattcgagct gggcaagctc aacctgcaag cggccctcgc gggtctgggc gtgccgggcg
      361 aagatgtcga ggatgaggcc cgtgcggtcg agcaacttgg tgccaagtac ctcttcaagg
      421 ttgcgctgct gcgtgggggt gagttcctcg tcaaacagca ccacgtccgc ctgaagctgc
      481 tctcgtaggg tctttatctc atcgaccttg cctttgccga cgagggtagc tcggttgggg
      541 tcgttgagtt tctgggtaat acggcccagc acctccacgc ccgcggtggc cgcaagctgc
      601 tctagctcgt cgagcgagtc ctccatgctc agcgtctgct tgccggtctc gacgcccact
      661 aatattgcct tttccacggg cttccccgtg tccgtcattc gagttgagat agtattctcc
      721 tcctggggcg acttgcgacg tcgtcgcctc cctccttatg agtgatccta aaactgcagc
      781 tttggaacgc gtgctattgc ctgatccact tcctcgtccg acaccgtaag cgaaatcctg
      841 aaccagcctt cgccctgcgc gccgtagctg gcacccgaag tgatcgcgac gccgatttgg
      901 gacagcatta gctcggaaaa ctgcgccgat gtgtagccgt ccggtacggg tgcccacagg
      961 tagaggctgg ctttgggtgg ctcgacgtgg atgccgattg ctcccagcgc gtgacatagc
     1021 ttgtcgcgcc tcttgcggta aatctcgttg cgggcgggtc tccaggactg gtcgccgctc
     1081 agcgccgcat aggctgcctc ctgcaccgcg ccgtaaatac cggagtctat gttcgtcttg
     1141 atacggccca gcgcacccac tatttccgcg ttgccgacga ccatgcctat acgcagcccc
     1201 gccatcgaat aggtcttgct cagcgaattg aactccaccg cgacatcttt ggcacccggc
     1261 acctgtagaa ttgagggtgc cgtatagccg tcgaaagtaa tctcgctata ggggttatcg
     1321 tgtgcgatta tgacatcgta gcgcttcgcc caggcgatga cccgctcgaa cacgtcaagc
     1381 ccggctattg cccccgtggg gttgttgggg tagttcagcc acagcacctt gacggccctg
     1441 gaagtgtccg cgtccagagc gtcgaggtcc ggcagccagc cgttctcggc ctttagcggc
     1501 agggggcata tcaccccatc ggccatgata gtgccgaact tgtaaaccgg gtaggccggg
     1561 tcgggcacta gggcggcctc accgggatct acaaaggcca gggcgacgtt ggcgataccc
     1621 tctttggagc ctatgagcgg gaggacttcg gtgggcggct caagctccac tccgaagcgc
     1681 acccggtacc agtcggcgac ggcctcacgc agctttttct ttccgtagta gtccgggtat
     1741 cgtgagtttt ccggcttctg agcggcctcg cacagggcgt cgagcaaaaa gcccggcgta
     1801 ggcacgtcag ggtcgcccat tgtgaggttg atcacctcgg tgccggaggc gcgcaggtcg
     1861 gctatgcgct ttgccatgcc cgcgaagacg tacggaggta gcttgttcag tcgctcggcg
     1921 aatctcatcc ttgagtcctt gtcggtgctg ttgggaggct cccacaatta taccccacag
     1981 cctatgccgc cacaaagcag ccggatgcat ccaagctaag ggttccctcg tacgcgctca
     2041 gcagcctcaa agaattgcgt caccaggcgt tgtgcctctg gaaatgggtc gctgtccgta
     2101 acgtcgataa gggcggcggc cttcgcacgc cgaaaccagt taccctggtg ccgcgagaag
     2161 ttgtgtgtgt cccacttgat gcgttgcgcc gcctgctcca gcgtggccct tcccgtgagg
     2221 tactcgccaa tctgccggta gcctatgccg ctcatggatg gcaaatcgaa gccgtagcca
     2281 cgggcgtgta gtgcggctac ctcgtctacg aggccacgct caagctggcg gtccacgcgc
     2341 agatcgattc tcctgtacag ttctggacgc tcgcagtgga gcactatagt cagtatccgg
     2401 tagggcgggg gcaccttgtc ctgttgctcg ctgatttgcc gtcccgtgcg cgctatcact
     2461 tctagggcgc gtatgatgcg gcgcgtattt gtcggaagga tgccctcggc ggcggctggg
     2521 tccagttccg ccaggcggcg atggagtggg ccggggccac ctgccgcggc ttcggcttca
     2581 agagtggccc gcaactccat gtcaggctcg acctcgggta tcgtccagcc ttcgagcact
     2641 gcgttcacgt acagcggagt gcctccggcc aggatgggaa cgttgccgcg ggccaggact
     2701 tcgttgatag tcgcataggc cccgcgctgg tacaaggcga gcgtgtacgg ttcgtctggg
     2761 tagaccaggt cgatcatgta gtgtgggagt cgggcgcgct cctccggcga gggcttctcg
     2821 gtgccgatat ccatcagctt gtagacctgc cgggaatcgg ccgtgacgat ctcgccgccg
     2881 aagtgctcgg ctagccggag tgcaagggcg ctcttccccg aagctgtcgg acctgtgacg
     2941 gccacgagtg gtggcaacaa tgaggacatg ggtacattat atggtttgcc cgccccatcg
     3001 ttaaggaccg acctcctcgg agtaacggcc cggtgtagtc gcttgccatg ttggccctgg
     3061 ctttgctaga atgacaactg gctaggaaag aaggagatac aagtaagtgc aagtcctggg
     3121 cagcataatt agaggggcaa tcggtctctc aatgatagtg gtcgttctgg cggcgggcgc
     3181 gggcatatac atgttcgtgg gcaacgcttt gcagtcgggg gagtctggag ctaccgactc
     3241 ggtgccggtc gcttttgaga tcaagccggg cgagggcgtg aacgacgtct cctcgaacct
     3301 ggagcggcag agcatcgttg acagcgcctt ttggttcaca cttcaactga ggagtagcgg
     3361 caaggacaag ctcataaagg ccggtacgtt tcaactgcgc acgaacatgg acaacagcga
     3421 agtgattgag gtgttgaccg cgccaatcgc tgacgagccg ggcgtgcgct tcacagtgat
     3481 cgagggcctg cgacttgagg agacggccga aaagctgggc gcgcagggcc tgataacccc
     3541 cagcatcttc ctagatgcgg taaggtcgcc ggaaggggtc gccaagtacg gaggcgcgtt
     3601 cttccaggag gccggtgcgc ccgccgacgc tacgcttgaa ggctacctgt tcccggacac
     3661 ctatgaaata aagcagaacc ccggcgacaa caccgacgcc gtaatcaaga tcatgctgga
     3721 cacaatgcag cagcgaatca cccctgagat gcgcgagaag ctgaccgcgc agggccgcac
     3781 ggtgcaccag atgctgacgg tcgcttccat cgtgcagcgt gagggtgttg tcaaagagga
     3841 gctgagcacc atcgcggcgg tgttctggaa ccgtgtggac gcgggaatgc agcttggcgc
     3901 ggacccgact atccaatacg cgatagggca gtcgcctacc tggtggcccc cgctaaacct
     3961 ggtcccgaac gaggtggaaa gcccgtacaa cacatacgtc atcatcggcc tcccgcccgg
     4021 ccccatctgc gcgcccggcc tggcagccat tgaagccacc gtgaatcccg aaaccaccga
     4081 ctacctgtac ttcgtagcca aggaagacgg cacacacacc cacgcgttct cccgcacgct
     4141 tgaggaacac gaaagaaacc gcgccctgta cggcaataaa tagccgctag actgcacgct
     4201 aatttggcag aatggcccct ggtacgtgat cgtaccaggg gcaattcgtc gcctgtatgg
     4261 agtatcactt acggctacga ccgtattttc taccagacct accggagaaa gaacccttcg
     4321 gccaccgggt cgtcagcgtc cactacccac tggcagaagc cgacgatgta accctgccca
     4381 gccacctcag gcacaaccgc cggaaagtca ccgaccattg tttcctcgac tacacgcgcg
     4441 gtgaagcgag tgccgataat gctttcgttg acgaactccc gtcccaccgg cagcagcccc
     4501 ctggcatgaa gctgggccac acgtccggcc gtgccggtgc ccgttgggga gcggtctacc
     4561 tcacggtccg cgaacacgca cacgttcgcc tggtctgcgc cgggagccaa aggcgggcct
     4621 gaaatgatgg tgccgtagat gccgtgcaaa gcaggctcca gcgggtgcac cacctcgatg
     4681 gcggcctcta cggcgtgctt cacctcgtcg cctagttgaa tcagcgggcg gtagtcggct
     4741 ggacgcaccg tcaggtccgc ctgttcggct tccaggtagg catagaaggc cccgccaaag
     4801 gctacgtcaa ccgtgagtct gccgaagcca ggagtgtcca cgaccagatc acgtcggtag
     4861 aggaaggagg gcacgttgag aaagcgggtg cgcccggtct tccgcccatc ccactccaca
     4921 aacgcctcga caaagccggc gggcgtatcc agccccacgc gcgtctcagg ggtagtggcg
     4981 gggaccatgc ccgtggcaac ggcgacggct gccagggcga tgacgccgtg cccgcacatc
     5041 gtgctgtagc cctcgttgtg catgaatatt acacccaggt ccgcattggg cgtgaccggg
     5101 ggagtgacgt agcagccgta catgtcggcg tggcctcgcg gctcgtgcat gagggcgcgg
     5161 cggacatggt caaggtgctc tcgcacccag ctacggcgct ccaggatggt agcgcctggg
     5221 atgaagggga tgcctgaggt gacgatgcgc agcggctctc ccgcagtgtg gcagtcaatc
     5281 gtcgaataga cgcggtcgaa gtgcatgccg cccactcctt ctgatttcga cagtctggcc
     5341 gcaacagctt agcacttact cctgggcggc gcaagacggt ggcccgccac ggcagggctt
     5401 cgttattctc cgttggttgg cgtggggcgc cctgtggctg aagccacggg cttacaagag
     5461 gcaaagtccc cttcgcggac taagcaacat catgtagtct ccgtaggaga ctttgctact
     5521 tcacagcccg ccagttcatt cgcagggcgt ccccccgcct cattgccgag aatgattaag
     5581 ccccgccacc gcagttcggg acaacaagaa gacctgctgg ttaggcaggt cttcttgttg
     5641 aggagggagg aaggaaggtt ggcaaagtta gggttgacct tactggtctt tgggccgggc
     5701 ggcgagggta atttgcaggt ccttctcgcc ggtcgcgccc ggttccagga cggtcagcgt
     5761 tacggtgtcg ccgactttgc gctccatcag aagctccatc agcgtattgt cttcgttcag
     5821 ctctacgccc tcgaacctgg tgatgatgct gccgggggtg atccctgcct tgtcagcggg
     5881 ggtgcccggc gagacctcag agacgagtat cccgtgatcg cgggccaggt tttcagcccc
     5941 tgccagccgc tcattgatca tctggtaagt gatgcctatg tagggcctcg acacgacgcc
     6001 gttgttgatg atctggtcca ccacagcccg cgccgtgttc gatggtatcg cgaagccgag
     6061 accttccgcg acgtcgccca ccccggtgtt gcgcacgacc gccacgttga tgccaatcac
     6121 gttgccctcg atgtcgagga gggggccgcc cgagttaccg tggttgatgg cggcgtcggt
     6181 ctggatcagg tcacgtagcg cggtggagtc ggcgtccttt atgtcgcggt gtacctggct
     6241 gaccacaccg gctgtaacgc tgttctggta gtccccgagg gccgagccga tagcgaccac
     6301 cggctgtccc ggctccagct tgtcgctgtc gccccagctt gccaccgcgg gcattttagc
     6361 atccaccttg agcaccgcga ggtccgagaa ggggtcggta cccacgaggg tggcggctgc
     6421 tttcttgccg tcggagaaga ccacttcgag gctgcgctgg ttcgccacaa cgtgattgtt
     6481 ggtaacgatg tagccccgcg cgtccactat gacgcccgaa ccactggcct ggggtatcgc
     6541 gccgaacgcg cccggctgag cgttggtgtc aaggtaattc acgaccgtga cgaccgccga
     6601 gcctgtcctg cgggcagcga tacgcgcgtc gagcacgccg ccttgtgtcg gtgccggggg
     6661 ttcgggggcc agcactgcgt tgtcggggct ggcgggttgc gcggcatcgt tgctcgccgg
     6721 ctttacctcg gcccccgtgc ccgcgcctgc ggttgacgga ggaccgaagc tcagagtgga
     6781 agcgatgcct gtgacggaaa ggccacccag caccaccagc aaggccagca gcagggaagc
     6841 cagtcccact cttacaacac gtccaaacat tgttctcgct ccatacgttc taggtagtct
     6901 aagatccctg aaaagtgcct gttctgaaag cagtcgctac tcttcgctaa ttgaataggt
     6961 caggcctctc ctcgtctttc gacctgctac cggggctgac agcttactgt cacacacccg
     7021 gcggcggaga ggcccgttgg ctaacggatc agggagcaca ctttacgacg accgcggcta
     7081 ggtcccattc cctaattgtt tatctcgcta ccagtgccct tactggtatg tgcgctccct
     7141 cgatgtcttc attatggcag ccgtccatta acgaaagctt agaggagtct tagaaacaga
     7201 ttagaatcac aggccaaaag gaaaggaggc atagacgcct cctttgaacg tcacacagtc
     7261 ctccgctaca ggccgccacc ggaagagcct gcgccgccct ctggctgctg ggcgaaccag
     7321 ctccaaactt cgttggactc ctggtctgac cagccgtagc tgcgggtcaa cgcgttccac
     7381 ccctgacggt cgtcttcggt gaactgcctc tgcaattcca gtatctgcgt ctccgatgcg
     7441 ttatcgagga ccggggggcg cgtcgtaccc gcgcctccgc cgctcgcgtc atccatgagg
     7501 gttctccttg gtgcttcgag gctgtaggtt gggccggtag ccccgcgcaa agcttagcta
     7561 atcgttgacg ttgacgttag tggtccccgt gccgggtccg ggtacgttca cggacaccga
     7621 gcctccaccg gccgtctcaa cacccgttgt gggtacgacg ttgatgttgg tggtctgcgg
     7681 cgcggcgggt tggacctcgg tggtcgtggt ggttgaggtg gtcgtggcgg tctccacggg
     7741 gactacggga acggccggca ctacttcggt tgaggtggta gttgtgcgtt ccgtgcgagt
     7801 tatctggtca gacattgttt ctccatcctt tcgcaagagt ggccgcgatc tggggcggca
     7861 tggccgatca ggcacccgcc caccgacagt aatgcaaaag gcatgccatt gtgccgcctc
     7921 agaagaccct cgcccccggc tcgacctcgc gctcaggtgc gagcaggata acgcctccct
     7981 cgcgagcgac cccagcgtcg ggcagaccca gcaccagcac ctcggaggcg aagcccgcga
     8041 tgcggcgtgg cgggaaattg accacggcca caaccatctt gcccaccagc tcctcaggtg
     8101 tgtagtgggc ggtcagttgg gcgctggact tcctcgtgcc gagcggtccg aagtcaattt
     8161 ccagcttata ggagggcttg cgcgcttcgg ggaacggttc gaccctggtg atgcgcccgg
     8221 cccgcatgtc tacgcggtcg aacacgtcaa tcgtggtggt ttcgggggcg cggtcgttcg
     8281 ggtcttgcat agtgccttgc tcctaagggt tgaggttggg gtggtctgcc gtgtcgagcg
     8341 gcaggtaagt cttggtctgg ctcggcagaa tgcggcctga gtagccgttc ttgaaaatct
     8401 cggcggcggg cttgggcgtg ccgtccagcc tgaagaggcc aaagtgctcc tcgaagttag
     8461 ccaccttcgc tgtggagcgc ggcgcgaaat ccataagcga ccactg
//
LOCUS       DASRWZ010000724         6087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_127252, whole genome shotgun sequence.
ACCESSION   DASRWZ010000724 DASRWZ010000000
VERSION     DASRWZ010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127252"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFH60_11440"
     CDS             <1..289
                     /locus_tag="VFH60_11440"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6314441.1"
                     /translation="RFVQALCGEETVGVGMAVSERGWTGIFNMATAQEMRGRGVATSI
                     ISDLARWSLEQGADRLYLQVMESNEVARRLYTRLGFSALYGYHYRTEPERS"
     gene            complement(532..918)
                     /locus_tag="VFH60_11445"
     CDS             complement(532..918)
                     /locus_tag="VFH60_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6314442.1"
                     /translation="MKDKDNMTKVLVVDDDPDLVAVCSLVLEYEGYDIAVARNGVEAY
                     KVLSEAGPNAPDVVLMDVMMPVMDGITVCKMVKRDPHTRDLPVIIMSASESLREQARN
                     ASADAVIAKPFDIDYLVSTVHQFAVS"
     gene            complement(1040..2539)
                     /locus_tag="VFH60_11450"
     CDS             complement(1040..2539)
                     /locus_tag="VFH60_11450"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET6314443.1"
                     /translation="MLGGGPKRIQQQHAKGKLTARERLDLLLDEGSFEELDMFVTHRI
                     TDFGMGDQKVLGDGVVTGWGRIDGRLVYVFSQDFTVFGGSLSEAHAQKIVKVMEMAMK
                     NGAPVIGLNDSGGARIQEGVLSLGGYADVFLQNVLASGVVPQISAIMGPCAGGAVYSP
                     AMTDFIFMVKDTSYMFVTGPNVVKTVTHEDVNFETLGGAVVHNTRSGVAHFAIDSEEE
                     CLLRIRDLLGYLPSNNLDDPPVVPNDDPPGRMDDELNSLVPDVPTKGYDMHEVIRRVV
                     DKGQFLEVHEHFARNIICGFARLGGQPVGIVAQQPLVLAGVLDIDSSDKAARFVRFCD
                     CFNIPIITFVDVPGFLPGVRQEHGGIIRHGAKLLYAYCEATVPKITVITRKAYGGAYD
                     VMNSKHVRGDINYAWPTAEIAVMGADGAVNIIFKDTIEQSDDPEATRKRLVDDYLEKF
                     ANPYVAAGRGFIDDVIEPRLTRPKLISALQMLRNKRDTNPPKKHGNIPL"
     gene            3037..6042
                     /locus_tag="VFH60_11455"
     CDS             3037..6042
                     /locus_tag="VFH60_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314444.1"
                     /translation="MKRLIVAMSAFVLVLIVARASSDELLGTTKAVAQANPTLPQISL
                     SNPETRQAVSFDVSPPLHSIPPRRAAVPAQQAGERENWIKPRSNVPNFVTTDPVVQHS
                     FGAEAMPTPILSFKGYTQHDNGDTGPLIVWPPDTEGDVGLNHYFQWNNIGFKIFDKSG
                     NLVYGPAAGNTLFAGMTGEGGAACEATNSGDPIVLFDTMANRWLASQFTTASLPTGPT
                     YECIAISKTDDPTGQYYRYAFLASPTSTPNFFEDYPHFGVWPDAYYMTTNEFALSKNV
                     ADFVGAGNFAFERAAMLAGNPAARMIYFHLNPPYGGLLPSDMDGPTPPPAGSPNYFME
                     VDDNFGEPPADLISVFKFHVDWTNVANSTFTGPTTVQVASFDAGLCEAERSACIPQPE
                     TEQKLEAITDRLMYRLAYRNFGTHESLVVNHTVDASGTGQAGIRWYELRSPGNSPTIH
                     QQGTYAPDASHRWMGSMSMDGDGNMALGYSVSNSTDLYPSIRYTGRLASDPLGQMSQG
                     EATMYQGLGAQLANARWGDYSMMGIDPVDDCTFWYTQEYYGSSGDYWLTRVGKFRFAG
                     CGGGAIPTETPVPAPNPCALPGAIVYRDAVGDVADLLGGGGSEIDKYDVEYISVAEPY
                     TGTGPNQLTFTLKVVSLDGPENDPATSPNSYWRIFFTKPFTTTLGFVDMRTNATGDVT
                     FNYGTGTGTTVGQADFGTYTREGSIRITVALSNLGLAPGGTISGIYSRVSNGAIFVDN
                     ANFPEPDWSAAYTIRGNAACQSGGVATPTPIVVPTACPIQFADVPPTGEGSTFYTYVR
                     CLACRQIVGGYPCGEPGEACNGSNQPYYRPGANVTRGQLSKIIANAAGLDDPVAPDQQ
                     QFADVEPGDPFYIFVERLAQTGAVEGYPCGGPGEPCTSLNRPYFRPSNPATRGQISKI
                     VAIAAGYNEDVPEDKQTFTDVPSDSPFWIYIERLSSREIISGYGTAGQCPTGTPCFRY
                     NDYTTRGQMAKIAANAFFPNCVTPARASP"
BASE COUNT         1220 a         1932 c         1809 g         1126 t
ORIGIN      
        1 cagattcgtg caggcattgt gtggcgagga gacggtgggc gtagggatgg cggtaagcga
       61 acgcggctgg acgggcatct tcaacatggc gaccgcgcaa gagatgcgcg gccgaggtgt
      121 tgccacgtcg atcatctcgg acctggcacg ttggagtctc gagcaaggcg cggaccggct
      181 ttatttgcag gtcatggaga gcaatgaagt cgccaggagg ttatacacta ggctgggctt
      241 cagcgcgttg tacggctacc actaccgcac agagccggag cgaagttaaa agaacaggag
      301 ggcacggaag aggcaaaaaa gaaaccccag gcaatccttt cggatcatcc ggagtttcaa
      361 atagtctttg gttcgaggcc gaagccccaa cccctctgac tggcctcagt ataaggccgc
      421 aggggtcgtg ggtcaatagg cggcccgatt ttttatgcac atcccaggct ccctggaagg
      481 gtagctgcta agctggttgc tcagcaaatg ggaaagcgat aacgtcgagt ctcaggagac
      541 cgcgaactga tggaccgtgc tcaccaggta gtctatatcg aacggcttgg caataaccgc
      601 gtccgccgag gcattcctgg cctgctcgcg cagcgactcc gaggccgaca tgatgataac
      661 gggcaagtcc ctcgtgtgcg ggtcgcgctt gaccatcttg cacacggtga taccgtccat
      721 cacgggcatc atcacgtcca tcagcaccac gtcgggcgca ttcggcccag cctcgctcag
      781 caccttgtag gcttcgaccc cgttccgcgc cacagcgatg tcgtacccct cgtactcaag
      841 caccaacgag cagacggcca cgagatcggg atcgtcgtca actacaagca cctttgtcat
      901 gttgtccttg tctttcataa ccatggtcct accacccccg gtgcagctac tttgcaaaaa
      961 gcatgccacc aaggggattt tagattttgg attttagatt gagaagaact cctaacaatc
     1021 caaaatctaa aatccaaaat caaaggggta tgtttccgtg cttcttgggt gggttagtgt
     1081 cgcgcttgtt gcggagcatc tggagggcgc tgattagctt ggggcgggtg agacgcggct
     1141 ctattacgtc gtcgatgaag cctcggcccg cggcgacgta agggttggcg aatttttcca
     1201 ggtaatcgtc taccaggcgt ttgcgggtgg cttcggggtc gtcgctctgc tcgatggtgt
     1261 ccttgaagat gatgtttacc gcgccgtccg cgcccatcac ggcaatctcg gcggtgggcc
     1321 aggcgtagtt gatgtcaccg cgcacgtgct tggagttcat cacgtcgtag gccccgccgt
     1381 aagccttgcg cgtgatgacg gttatcttgg gcacggtggc ctcgcaatag gcgtatagca
     1441 gcttggcacc gtggcggatg atacctccgt gctcctggcg cacgcccggc aggaagcccg
     1501 gcacgtctac gaaggtgatg atcgggatgt tgaagcagtc gcagaagcgc acgaaccggg
     1561 cggccttatc cgacgagtcg atgtcgagca cgcccgcgag caccagcggc tgctgagcca
     1621 caatcccgac cggctgcccg ccgagcctgg cgaagccgca gatgatgttg cgggcgaaat
     1681 gctcgtgcac ctccaggaac tggcccttgt ccactacccg ccggatgacc tcgtgcatgt
     1741 cgtagccctt ggtgggcacg tccggcacga ggctgttcag ttcgtcgtcc atgcggccgg
     1801 gcgggtcgtc attgggcacc acaggagggt cgtccaggtt gttggagggg aggtagccga
     1861 gcaggtcgcg tatccgcagc aggcactcct cctcgctgtc aatcgcgaag tgcgccacac
     1921 ccgaccgggt gttgtgcacg accgccccgc ctagcgtctc gaagttcacg tcctcgtgcg
     1981 tgacggtctt tacgacgttg gggccggtca cgaacatgta cgaggtgtct ttcaccatga
     2041 atatgaagtc ggtcatcgcc ggggagtaga ccgccccgcc cgcgcacgga cccataatgg
     2101 ccgagatctg cggtacgacg cccgatgcga gcacgttctg aaggaacacg tctgcgtagc
     2161 cgccgaggga aagcacgccc tcctgtatgc gcgccccgcc ggagtcgttc aggccaatca
     2221 cgggcgcgcc gttcttcatc gccatttcca tgaccttgac tatcttctgg gcgtgcgcct
     2281 cggatagcga gccgccgaac accgtgaagt cctgggagaa cacgtacaca agcctgccat
     2341 cgatgcgccc ccagccggtc acgacgccgt ccccgagcac cttctggtcg cccattccga
     2401 agtcggtgat ccggtgggtg acgaacatgt caagctcttc gaacgagcct tcgtcgagca
     2461 acaggtccag ccgctcgcgc gcggtaagct tgcccttggc gtgctgctgt tgaattcgct
     2521 tcggaccacc gccgagcatc gcctgctccc gcaaatcgcg cagcctgttg atgcgttcgg
     2581 cggcattggg tggcgcggca ggcgtcgagc cgttgggtga agtggcaggg gctgcgctct
     2641 ccgtgggggc tacgtcgtac gggctcgcct cgcggctata ctcggagatg ggcaactcgt
     2701 cgccgggccg tgtgggatcg gtttctcgcg tcatgggcgc tatccttcct ggggtgcgtg
     2761 taggtaacag cagccattat aacggctaga ggcacgacgc gccacgcacg ctgggccact
     2821 cacaagagcg agttcaagag cgagttttgc gccgccaacg ggcacctgct tcagttctcc
     2881 cggaacggct ccccggtttc caggaacgca tcttgctagt cctttcacct gccggcgcac
     2941 accagccgcc gacctgtgca ttcaagtagc catcgtgtgt ggtagagccg agcccaatga
     3001 cgggccggtt ttgatgacca ggaaggagag aaggctgtga aacgactgat cgtggcaatg
     3061 tcagcgttcg tactggtgct catagtggcg cgcgcctcgt ctgacgagct gctgggcacg
     3121 acaaaggctg tcgcgcaggc aaaccccacc ctcccgcaga tatccctgag caaccccgaa
     3181 acgaggcagg ccgtcagttt cgacgtgtcc cctccgctac actcgatacc ccctcgacgc
     3241 gcggcagtac ctgctcagca agccggggag cgcgaaaact ggattaagcc gaggagcaac
     3301 gtgcccaact ttgttaccac cgacccggtg gtacagcact ccttcggcgc agaggcgatg
     3361 cccaccccaa tcctgtcgtt caagggctac acccagcacg acaacgggga taccggtccc
     3421 cttatcgtct ggccgcccga cacggaagga gatgtgggac tgaaccacta cttccagtgg
     3481 aacaacatag gtttcaagat cttcgataag agcggcaacc tcgtctacgg acctgcggca
     3541 ggtaatactc tattcgcagg catgaccggg gaaggtggag ccgcatgcga agccaccaac
     3601 agcggcgacc ccatagtcct cttcgacact atggctaacc gctggctcgc cagccagttc
     3661 actacggcga gccttcccac cggcccaacg tatgaatgta ttgccatttc caagacagac
     3721 gacccgacgg gccagtacta tcgctatgcc ttcctcgcca gccctacctc tactcctaac
     3781 ttcttcgagg actacccgca cttcggggtg tggcccgacg cctactacat gaccaccaac
     3841 gagttcgccc tctccaagaa cgtagccgac ttcgtcggag cgggtaactt cgcgttcgag
     3901 agagccgcga tgctggcggg gaatcctgcc gccaggatga tctacttcca cctcaaccca
     3961 ccgtacggag ggctgctgcc atctgatatg gacggcccga ccccgccacc cgccggcagc
     4021 cctaactact tcatggaggt agacgacaac tttggcgagc cgccggccga cctgatctcc
     4081 gtattcaagt tccacgtgga ctggacgaac gtcgccaatt ccaccttcac aggtccaact
     4141 acggtgcagg tcgcatcttt cgacgccggt ttgtgcgagg ctgagcgaag cgcctgtatc
     4201 ccacagccgg aaaccgagca gaagcttgag gccatcaccg accggctgat gtacaggctg
     4261 gcttatcgca acttcggcac tcacgagtcg cttgtggtca atcacacggt tgacgccagt
     4321 ggcacgggcc aggcaggcat tcgctggtac gagctgcgaa gtcccggtaa ctcacccacc
     4381 attcaccagc agggcactta cgcccccgac gcgagccatc ggtggatggg cagcatgtct
     4441 atggacggcg acggcaacat ggccctcggc tacagcgtgt ccaactccac ggacctgtac
     4501 cccagcatcc ggtacaccgg caggctggcg agcgacccgc tggggcagat gtcacagggt
     4561 gaagccacaa tgtaccaggg gcttggcgct cagcttgcca acgcccgctg gggcgactac
     4621 agcatgatgg gcatcgaccc tgtggacgac tgcacattct ggtacaccca ggaatattac
     4681 gggtcaagcg gcgactactg gctgacgcgc gtcggcaagt tcaggtttgc aggctgcggc
     4741 ggcggcgcga tacccaccga aacgcctgtg cccgccccta atccctgcgc gctgcccggt
     4801 gccatagtgt acagggatgc cgtgggggac gtagcagacc tgctgggtgg cggcggctcg
     4861 gaaatagaca agtacgacgt cgagtatata tccgtcgccg aaccgtacac cggcacgggg
     4921 ccaaaccaac tgacgttcac actcaaggtg gtgagcctgg atgggccgga gaacgacccc
     4981 gccaccagcc cgaactctta ctggcgcatc ttcttcacca agccatttac caccactctg
     5041 ggcttcgttg acatgcgcac caacgccacg ggcgacgtga ccttcaacta cggcaccggc
     5101 actggcacca ccgtggggca ggcggacttc ggtacctaca cccgcgaggg cagcatccga
     5161 attaccgtag ccctctccaa cctggggctg gcaccgggcg gcactatcag cggaatctac
     5221 agcagggtat ccaacggcgc gatcttcgtg gacaacgcca acttccccga acccgactgg
     5281 agcgctgctt acacgattcg gggcaacgct gcctgccagt cgggcggcgt agctacaccc
     5341 acgcccattg tcgtacctac ggcgtgcccc atccagtttg ctgacgtgcc gcccacgggc
     5401 gaaggctcga ctttctacac ctacgtgcgg tgccttgcct gccgccagat cgtaggcggt
     5461 tacccgtgcg gcgagccggg agaggcctgc aacggcagca accagcctta ctacaggcct
     5521 ggcgcgaacg tcacgcgcgg gcagctatcg aagattatcg ccaacgcggc gggcctcgac
     5581 gacccggtcg cgccggacca gcagcaattc gctgacgtgg aaccgggtga cccgttctac
     5641 atcttcgtag agcgacttgc ccagaccggg gcggtagaag gctatccctg cggcggcccc
     5701 ggcgagccgt gtacgtcgct gaacaggccc tacttccgtc ccagcaaccc cgccacaagg
     5761 gggcaaatct caaagattgt agccatcgcg gcaggctaca acgaggacgt gcctgaggac
     5821 aagcagacgt tcacggacgt accttccgac agccccttct ggatctacat cgagcgactc
     5881 tcctccaggg agattatctc cggctacggc acagccggtc agtgcccgac gggtacgcca
     5941 tgcttccgct acaacgacta caccaccagg ggccagatgg cgaagatagc cgctaacgcc
     6001 ttcttcccca actgcgtcac tcccgcacgg gcctcgccct gacaagcacg catacggggt
     6061 aacctgtaac atgaagaagg ggcctcc
//
LOCUS       DASRWZ010000725         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_58265,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000725 DASRWZ010000000
VERSION     DASRWZ010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58265"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFH60_11460"
     CDS             complement(<1..656)
                     /locus_tag="VFH60_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314445.1"
                     /translation="MISALLILLVSALLCAALGFLPRMTVLARIPAVLGAAAALAVAL
                     LHEGLQGGGVAGAILPWPQALTWLGEPLYRSDTLSAGLGLWCLLAGGVCLLRIGEGHN
                     APRQLATALLTLATLYSITYTQHLLAFAGQVLLLALLVWAYHAWDASQQAQAGTGRHF
                     VSLGLGAIMLLSAVLLVGRTTGGAYHLSDLSLSALTVWPLSLLALFTALWLGTVPFTG
                     "
     gene            complement(687..1763)
                     /locus_tag="VFH60_11465"
     CDS             complement(687..1763)
                     /locus_tag="VFH60_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314446.1"
                     /translation="MLPAPMRLRLELRPAGDSSPLGWGAQIGSVEVERGYNYVGLEER
                     VRSGGVDWQSALALVEGTCGCCSQANLLAFVQAAEAMANLIVPPRAAYLRLVLAETER
                     IVSHLLNAADTMRALGLHEKGVSLLDLRERIVHALAEWSGARSQPGLIVYGGVASNPE
                     ESDVRALTLAARGVERALRAQVTATINSREAAGRLVGLGVITAQEAVVAGLRGPVLRG
                     SGVTTDIRATFTTGAYEDEAVTIVVQRAGDAFSRLIVRLLECLESLRVIEQAMDDLPT
                     GPFRGRGDMELRTGSAVGRAEGPRGEVFCWAWGGEGGLRGLHLSAGSFPSLGIVPGLL
                     KGQRLEDLPLLLLSLDLCVPSTER"
     gene            complement(1898..2419)
                     /locus_tag="VFH60_11470"
     CDS             complement(1898..2419)
                     /locus_tag="VFH60_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314447.1"
                     /translation="MDQDSNAAPRTGAFTRPMPGRDPDAEPAGVERQLAQYAFIREER
                     DREVRLLFRDVPADEVARAFRASGASLITIYGERLTPAVPTSDDVSGEAGTPPGEDGT
                     APRRHRKRRAESQSTPVGEALLRYFYSLGEIVYTVGIAVPSGVVASVAGVYPVAALSE
                     HEIRDRIAVVFKP"
     gene            2723..3238
                     /locus_tag="VFH60_11475"
                     /pseudo
     CDS             2723..3238
                     /locus_tag="VFH60_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          676 a         1106 c         1063 g          584 t
ORIGIN      
        1 cagccggtga agggtaccgt gcctagccac agggctgtaa agagggccag cagcgagaga
       61 ggccacacgg tcagggctga gagcgacagg tcagacaggt ggtatgcgcc tcccgtggtg
      121 cgcccgacga gcagcaccgc actcaatagc atgatagcac ccaggccgag ggatacaaag
      181 tgtcgtccgg tgcctgcctg tgcttgttgg gaagcatccc aggcgtgata tgcccatacc
      241 agcagggcca ggagcaagac ttgcccggca aacgccagca ggtgctgcgt atatgtgatg
      301 ctgtacaggg tggctagcgt caggagggct gtagctagtt ggcgcggcgc gttgtggcct
      361 tcccctatcc tcaagaggca cacgccaccg gccagcaggc accagagacc cagccccgcc
      421 gacagcgtat cgctccggta tagaggctcg cccaaccatg tgagcgcctg cggccagggt
      481 aatatggcac ccgccacgcc gccaccctgc aagccttcat gcagcagcgc gacggccagc
      541 gcggcggcag cgccaagcac ggcaggtatc ctggctagca ccgtcatgcg cggtaggaag
      601 cccagcgcgg cgcagagcag ggccgaaacc agcaggataa gtagtgcaga aatcatgaat
      661 tagattacag cggcaaggct agcaagctac cgctcagtag atggcacgca caggtcgagc
      721 gacaggagga gcaggggcag gtcttccagc ctttgcccct tcaataagcc gggcactatc
      781 cccagggatg ggaaagaccc cgcgctaagg tggaggccgc gcaagccgcc ctctcccccc
      841 cacgcccagc agaagacctc tcccctcggc ccctcggccc tccctaccgc gctgcccgtt
      901 ctcagttcca tgtcgcctcg ccccctgaac ggcccggtag ggaggtcgtc catcgcctgc
      961 tctattacgc gcaggctctc aaggcactct aagaggcgca ctatcagccg ggagaaggcg
     1021 tcgcctgccc tttggactac tatcgtgacc gcttcatcct cgtaagcccc ggtcgtgaag
     1081 gtggctcgga tgtctgtagt gacgccggaa cctctcagga cggggccgcg cagcccggcc
     1141 acgactgctt cctgagccgt gatgaccccc agtccgacta acctgcccgc cgcctcccgg
     1201 ctgttgatag tggccgtgac ctgcgcccgc agcgcgcgct ccaccccgcg tgcggcaagg
     1261 gtgagggcac gaacgtcgct ctcttcaggg ttgctcgcca cgccgccata tacgataagg
     1321 ccgggctggc tacgcgcccc gctccactca gcgagggcat gcacgatgcg ctcccgcaag
     1381 tcgagcagtg acaccccttt ctcgtggagg ccaagcgccc gcatcgtgtc ggcggcgttg
     1441 agcaggtggg aaacgatgcg ctcggtttcg gcaagcacca gccgcaggta ggcggccctc
     1501 ggaggcacta tcaggtttgc catagcctcg gcggcttgca cgaaggccag caggttagct
     1561 tgcgagcagc agccgcaagt cccctctacc agcgccaggg ctgattgcca atccaccccc
     1621 ccgcttctga cgcgctcttc caggcccacg tagttgtagc cacgctcgac ctctaccgag
     1681 cctatctgcg cgccccagcc caggggacta ctatcccctg ccgggcgcag ttccagccgc
     1741 aggcgcatag gcgcgggcag catgggatca tatgggccta tggtacagga cgtggagcct
     1801 gcgtgcgcct gggacgggac gaatggattg gaatacgtgg attgcaatct tgttatcaat
     1861 cccaaataga agtaagcagc ttatcagcgt cgtgagacta cggtttgaat acgactgcga
     1921 tgcggtcccg tatctcatgc tcggagagcg ccgcgaccgg gtaaacgccc gccaccgacg
     1981 cgacgacgcc cgaaggcacc gctatcccga ccgtgtaaac tatctcgcca agcgagtaga
     2041 agtagcgaag gagcgcctcg cctaccgggg tagactgtga ctccgcgcgg cgcttgcggt
     2101 gccgcctggg agcggtgccg tcctcccctg gtggcgtgcc tgcttcccca ctcacatcat
     2161 ctgaggttgg cacagccggg gtaagcctct cgccgtagat ggttatgagc gatgcgccag
     2221 atgccctgaa ggcgcgtgct acctcgtctg cgggcacgtc acgaaagagg aggcgcactt
     2281 cacggtcacg ctcttcgcgg atgaatgcgt actgggcgag ctgccgctcc acgcctgccg
     2341 gttcagcgtc agggtcgcgc cctggcatgg gccgtgtgaa agcgccggtg cggggcgcgg
     2401 cgttactatc ctggtccata attgtccaca ctttcccctt tcaggcggca cctgtggctc
     2461 cggccaatat accagtgttg agaggggcga gtcaagcgag gaatagtgtt aagttagggc
     2521 aaacgccgcc aaaatgacgg gaccataaac tggaacaact aagtacgaaa ccgccaagac
     2581 gccaagagca ccaaccaccc gccaagttct tgtgttatgg atacttacag cgtctttcaa
     2641 aaccgagcca atcttgtaaa atcaggatac agtatagatg aaggtaagga atgctaaaat
     2701 ttgtgttcgt gaaaggtgcg gcatgatcct cgccaaagat cgcgaccctc gcttcatcac
     2761 catccgccgc ggtgggacgc tcacagattc agaccatcat cttctcgcct cgtgggcggc
     2821 cgcatgtgcg gagcacgtcc tgcacctctt cgagtctgcg aggccttcgg actcgcggcc
     2881 gcgtcacgcg attgagcagg ctcgggcatg ggcgcgcggc tagatcccga tgtcccaggc
     2941 ccgcgcgtcg gctggccatg cgatggcggc ggccagggat ctgagaggcg cggcacgcca
     3001 tgccgcgtat gctgccggtc aggccgcggc ggtcgcgcac gtcgccgcgc acgagctagg
     3061 cgccgcagcc tacgcgatca aggccgcgcg cgcggctgcg cctgaaggcg agagcgagag
     3121 tgcaggccgg cttgagtgcg actggcaacg cgaccagttg ccggatgaaa tccgcgatct
     3181 tgtcctggac gatcagcggt tgcgaaacga catctgctgg tcggtattcg actgctgatg
     3241 ggcgaccggc gtgtgccggc actgcccaac tagcgcttcg agccgacgcc gaactcaaac
     3301 cgcgaaacga ggtagcctgg gggccacaac cctataaaat acctggcgct cctggcggtc
     3361 tcgttaccac tacaatttag atacggctgc cgtaagccat ttcattgcat gacaggctac
     3421 ctagcgcgc
//
LOCUS       DASRWZ010000726         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_744027, whole genome shotgun sequence.
ACCESSION   DASRWZ010000726 DASRWZ010000000
VERSION     DASRWZ010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744027"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VFH60_11480"
     CDS             complement(<1..1071)
                     /locus_tag="VFH60_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314448.1"
                     /translation="MSVVPPPPIPTRTSSRPAPAAAQRRLNPVERALAGPPPRYARPL
                     LMFLLAVGLLAAALWGALTLGGGVEMRLVLQNNFNAATNELPTSMVAQSFYVTRPNLS
                     RLDIAMSFVPVLDSDGRVRLLEGEGLGGAVLYEVPLRQTRFTQGLYLSVDLPPQPDSQ
                     GRTYTVVVETPGRPLGETVGIRYNTFDALTAGGMYTNDSDGRPTGGDLSFVMYYRYGM
                     GTLLADVMNVAGEARRLALSPLLLLLLPGLALLVWLPNSLTGGQRLLAAPGLSALALP
                     VFFLVTRALGVKMGEAQMWALMVLCAVVLGAGLWRARPKLNWRGIKGEDAAFWSLLLG
                     VLGATIISRFVALRDQVGGVGLD"
     gene            complement(1084..2337)
                     /locus_tag="VFH60_11485"
     CDS             complement(1084..2337)
                     /locus_tag="VFH60_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET6314449.1"
                     /translation="MMDMDEIDVLEVPVDRTPRAELEGRATRLQAAMREAGLDGVLAT
                     QNADVFYLSGIVQQAQVYLPVAGRPLLMVRKHFDRARITTELDESQVVGVRSLRELPA
                     LLESVGARPRRLGLELDTLPYNTYQAYAKALSPLEADLVDASMLFRQVRAIKSEFELD
                     QIRTAARVADVGIRAASEAAVEGMAEVELAAIIEAATRVAGHSGILRMRFFGQEMHMG
                     HVLAGNAGGVASFMNSPTGGYGVGPWAPYGASRRPIRRGEPVFIDYTGEWGGYIADQT
                     RMLSIGPLSHFWQDVYVAMVDVEAYVAANARPGMTSGELFSLAEERAGHLGYADYFMG
                     PPEAESAGQKVPFIGHGVGLELDEWPPLQRGTDAVLQAGMVLALEPKLIYPNRGAVGI
                     EDTYVLTDQGLSPLTISSPQIFVVD"
BASE COUNT          431 a          837 c          835 g          381 t
ORIGIN      
        1 gtcgaggccg acgccgccta cctggtcgcg cagcgccacg aaccggctga tgatggtcgc
       61 ccccagcacg cccagcagga gcgaccaaaa ggccgcgtcc tcccccttga tgccccgcca
      121 gttgagtttc ggccgcgcgc gccacaggcc ggccccgagt acaacggcgc agagtaccat
      181 gagcgcccac atctgcgcct cgcccatctt gacgcccagg gcgcgcgtca ccaggaagaa
      241 cacgggcagc gccagggcac taagtccggg cgcggccagc aacctctgcc cgcccgtcag
      301 gctgttgggc agccatacga gcaaggcgag gccgggcagc aacaacaaga gcagcggtga
      361 aagcgccagc cgccgcgcct cacccgccac gttcatcacg tcggccagca gagtgcccat
      421 gccgtagcgg tagtacatga cgaaggacag gtcgccgccg gtgggcctgc cgtcgctgtc
      481 gttggtgtac atgccgcccg ccgttagcgc gtcgaaggtg ttgtaccgta tccccacggt
      541 ctcaccgagt ggcctgccgg gggtctccac tacaacagtg taggtgcggc cctgcgagtc
      601 gggttgcggg ggcaggtcca cggacaggta cagcccctgc gtgaaccgcg tctggcggag
      661 cggcacctcg tacagcaccg cgccacccag gccctccccc tccagcaagc gcaccctgcc
      721 gtcgctatcc aggaccggca cgaagctcat cgcgatgtct aacctggaaa ggttggggcg
      781 cgtgacgtag aagctctgcg ccaccatcga ggtaggcagc tcattggtcg cggcgttgaa
      841 gttattctgg aggaccaggc gcatctccac gccgcccccc agcgtgaggg caccccagag
      901 cgcggcggca agcaggccca cggccaggag gaacatgagc aacggccggg cgtaacgagg
      961 cggcgggcct gccagcgccc tctccaccgg gttgaggcgg cgctgggcgg ccgcgggggc
     1021 ggggcggctg ctcgtcctcg tgggtattgg gggcgggggg actaccgaca tgtcagccag
     1081 ctatcaatcg accacgaata tctgtggcga cgaaatagtc agggggctaa ggccctggtc
     1141 cgtgagcacg taagtatctt cgatgcctac cgcgccccgg ttggggtaaa tcagcttggg
     1201 ttcgagggcc agcaccatac cggcctggag caccgcgtcg gtgccgcgct gcaacggggg
     1261 ccactcgtcc agttcgaggc ccaccccatg cccgataaag ggcaccttct gcccggcaga
     1321 ctcggcttcg ggcggcccca tgaagtagtc ggcatagccc aggtgccccg cgcgctcttc
     1381 agcaagggag aagagttcgc cgctggtcat acctgggcgg gcgttggcgg cgacgtaggc
     1441 ctcaacgtcc accatagcga cgtagacgtc ctgccagaag tgggagagtg ggccgataga
     1501 gagcatgcgg gtctggtccg cgatatagcc gccccactcg cctgtgtagt cgataaacac
     1561 cggctcgccc cggcgtatgg ggcggcggct ggccccatag ggtgcccagg gaccgacgcc
     1621 atagcctccc gtgggcgagt tcatgaagga ggcgaccccg cccgcgttac ccgcgagcac
     1681 gtggcccatg tgcatctcct ggccgaagaa gcgcatacgc aggatgccgc tatgcccggc
     1741 cacgcgggtg gcggcttcga tgatggcggc aagctctacc tcggccatgc cttccaccgc
     1801 ggcttctgag gcggctctga tacccacgtc cgcgaccctg gcggcggtcc ttatctggtc
     1861 cagttcgaac tcgctcttga tcgcccgcac ctggcggaac agcatgctgg cgtccaccag
     1921 gtcggcctcc aggggcgaga gagccttcgc gtaagcctgg taggtgttgt agggcagggt
     1981 atcaagctcc agccccagcc gcctgggccg cgcgcccacg ctttccagca gggcaggcaa
     2041 ctcccgcagc gaccgcacgc ccaccacctg cgactcgtcc agctcggtag taatgcgggc
     2101 gcggtcgaag tgcttgcgca ccatgagcag gggccgtcct gcgacgggca ggtagacttg
     2161 cgcctgctgc acgatgcccg acaggtagaa gacgtcggcg ttctgcgtgg cgagcacgcc
     2221 gtccagcccc gcctcgcgca tggcggcctg caagcgtgtg gcgcggccct ccaactcggc
     2281 gcgtggagtg cgatctacgg gcacctcaag cacgtcgatt tcgtccatgt ccatcatagg
     2341 ggcagcatag aggaactacc gccgggtgtc aagttgcctt cggtgaaggg ttgaagtgca
     2401 ggcgtgctca gtggagaacg taggggatgc cctgggatag gcctgtgggg acgccccgta
     2461 ttgacgccct gtggctgaag ccac
//
LOCUS       DASRWZ010000727         5014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_881513, whole genome shotgun sequence.
ACCESSION   DASRWZ010000727 DASRWZ010000000
VERSION     DASRWZ010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881513"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            662..2128
                     /locus_tag="VFH60_11490"
     CDS             662..2128
                     /locus_tag="VFH60_11490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6314450.1"
                     /translation="MRIAFFSPLNPIQSGISDYSEELLLALHGARVAGSPAYIDLFID
                     KGYKPSNTDVVRRFDVRQGRAFGRVAGQYDSVLYQMGNSAAHAYIYDALLRHPGVVVM
                     HEFVLHHLRVWMTLNSGKRKEYMAIMERQYGAEGLDAARRVLLGQYPEALFNYPLSDE
                     VIRAARGIIVHSRYMEELVKGVRPDVPISVVPMGVPLPPRIPRDEARARLGLDPGLFL
                     VTSVGPLNPYKRVSATLRAFRALLREVPDSAYLLIGSRSPNYDPTRQIEMLGLTERVR
                     STGYVSPQDFPYYLAASDVCLNLRYPPAGETSASLLRIMGAGVPVLVSRTGSFEERPA
                     DAAGKVDVGDFEEELLLEYLLLLARRPDLREAMSASARCHVAERHPLEGAATGYLRFL
                     ASLSSRGGSEPTITLSPDAATAVHVPSPAGSIARVQIGQAVAPPPATPARKLPDNDTI
                     AVVAEAAAELGIEESDTWLLADVAKAVEGMVPGAREEW"
     gene            2122..4677
                     /locus_tag="VFH60_11495"
     CDS             2122..4677
                     /locus_tag="VFH60_11495"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET6314451.1"
                     /translation="MVAAAKRQAERRVAVWRGQGVREVALLAVLPVLISFAMLWPALL
                     GQGVLASTDMVTYDPFIGDGRPGVQPPISANPVLGDPVDQFIPRRLYARGELRAGRFP
                     LWNPYNALGTHFHANYQSQVLSPFNILWLALPPIWGLGFIAALKWALMGLGMGLLLRR
                     LGLGMPAAVFGSVAGQLMGPMVAWLQWQHTEALAWMPWLLLAALAWVDTRRLLWLAAF
                     GALVAAELLAGHVETAFYSFALGGAFALAAGGAAGMPPRQKWLSLAGLLGAGVLGAGI
                     AAAQFLPLLGVLTSSWQWVAREGSTTHLTSLPPEAALTYLSPNGFGSAGSWYGPLNWI
                     EINGYVGALTLLLAVWGLAAAIFARRAPDAQADPTFPVRLGQALSPRKQLFWVLAAIT
                     GISMAYGIPPFSLLRNLPGFSSSLNYRLIAVAAASLVVLGAMGLQRLSEYRVPSAEYR
                     VGPLPLRTRYSVLGTFLLAGLGLLFLVDGVRAWYVDSAAAGAYMQAWKMWAGALFCAG
                     GALILARVSGLLGTRAFAALAVVLLLLDLGRANWNFNVTSPLGTFYPANEMTDFLAQR
                     GITERVAIDAAWAEPNRLMAYRIPDIRYYDPTIDNRYRVFIRLMSPGTFTSPYPAYPI
                     HLMLDRPSATLLSMVGVKWLVTTSNFNPNTWQPAPSKGPIYAPWLNRNSFVAWENRYA
                     NPYSYLASRIRVVPDEAAARQRMSEMKLEEINEATVEHPAGLPGEVASASNGQPLTEA
                     EVDSLETVANIPGTIRVRLRTEKARLLVINESWSPGWRATVDGTETPIYRANYIVQGV
                     VVPPGEHEVVFHYDPPIFKLGLAVSGVSLLGWLGLLALAIRRWRRAREILPGG"
BASE COUNT          836 a         1716 c         1594 g          868 t
ORIGIN      
        1 gttcgctcct tgctcaacct acccccggaa gtcaccccga tgggcgtcat ccccataggc
       61 caccgcgctc ccgacgtgcc ctccccctcc ctaaagcgcg gtcgcaagaa gctggagcag
      121 caggtctact acgagagctg gggagccgaa acgcagtcgt gatgcgtgaa gcgtactgcg
      181 taacaacgct taccacccag gaacgtgaat acgcgccgcg ggcctaaaag gaccacactc
      241 ccccttgtca tcctgagcgt agcgaaggat caggtgggta ggccagaggc tttaacattg
      301 tggatgatgg cggctaaagg aacattggtg ccccaagagg aagggtcaag agcgggccac
      361 tgattcttcg ctgcgctcac tggagcattc ggtaagtaag tgcccaaagc aggagcaaag
      421 gaagtattgt cattccgagc gtagcgagga atcaggtggc agtcgtactc agtagcattg
      481 cggcaggcaa gcggcgatgg gcaagggcac gccgctactc ggcactgcaa ttcgccttct
      541 tccctaccca cctgattcct cgccacactc ggaatgacaa aggtcccttt gctcctgccc
      601 caggtagcta tgtgggctaa tcggacagcc tgcctccgga tgctctacaa tagtcactgc
      661 catgcgaatc gccttcttct ccccgctcaa ccccatacag tccggcatct ccgactacag
      721 cgaggagttg ctgctcgccc tccacggtgc ccgcgtggca ggctcgccgg cctatataga
      781 cctgttcatc gacaagggct acaaaccctc caacacggat gtcgtccggc ggttcgacgt
      841 gcggcagggg cgggcgttcg ggcgcgtggc gggtcagtac gactcggtcc tctaccagat
      901 gggcaactcg gcggcccacg cctacattta cgacgccctt ttgaggcacc ccggcgtcgt
      961 agtcatgcac gagttcgtgc tgcaccacct gcgggtctgg atgacgctca acagcggcaa
     1021 gcgcaaagag tacatggcaa tcatggagcg gcagtacggc gcggaagggc ttgacgccgc
     1081 acgcagggtg ctattaggac agtacccgga agccctgttc aactacccgc tcagcgacga
     1141 ggtgatacgg gccgcgaggg gcataatcgt gcacagccgg tacatggaag agctggtaaa
     1201 gggcgtgcgg cccgacgtgc ccatatcggt agtgccgatg ggcgtgccgc tgcccccccg
     1261 catacccagg gacgaagcga gggcgcggct gggcctcgac cccggcctct tcctggtcac
     1321 ttcggtgggc cccctgaacc cctacaagcg ggtgtcggcc accttgcggg ccttcagagc
     1381 cctgctgagg gaggtgcccg actccgctta cctgctcata ggcagccgct cgcccaacta
     1441 cgaccccacc cgccagatag agatgctcgg cctcaccgag cgcgtgcgca gcaccggcta
     1501 cgtgtcgccc caggacttcc cttactacct ggccgcttcc gacgtgtgcc tcaacctgcg
     1561 ctatcccccg gcgggcgaga cctcggcctc gctcctgcgc atcatgggcg cgggggtgcc
     1621 cgtgctcgtg tcgcgcacag gctcgttcga ggagcgaccc gccgacgcgg cgggcaaggt
     1681 ggacgtgggc gacttcgagg aagagctgct cctggagtac ctgctcctgc ttgcccgccg
     1741 ccccgacctg cgcgaggcta tgtccgcctc ggcccgctgc cacgtagccg agcgtcaccc
     1801 gctcgagggc gcggcgaccg gctacctgcg cttcctggca agcctctcca gccggggcgg
     1861 gagcgagcct actattaccc tctcaccaga cgccgccaca gccgtgcacg taccttcccc
     1921 agccggcagc atagcccgcg tacagatagg gcaggccgta gccccaccgc ccgctacccc
     1981 cgcccgcaag ctgcccgaca acgacacgat agcggtagtg gccgaggccg cggcggaact
     2041 gggcatcgag gagagcgaca cctggctgct ggcagacgta gcgaaggcgg ttgaaggcat
     2101 ggtgcccgga gcgcgggagg aatggtagcc gctgcgaaac ggcaagccga gcgcagggtt
     2161 gcggtgtggc gcgggcaggg ggtgcgggag gtagctctgc tggctgtcct gcccgtgctc
     2221 atctcgttcg ccatgctctg gcccgccctc ctgggccagg gggtgctcgc ctccaccgac
     2281 atggtgacct acgacccatt catcggcgat ggcagaccgg gcgtgcaacc gcccatctcg
     2341 gccaaccctg tgcttggcga ccctgtggac cagttcatcc ccaggcggct ctacgcccgc
     2401 ggcgagctta gggccgggcg cttccccctg tggaacccct acaacgccct cggcacccac
     2461 ttccacgcca attaccagtc gcaggtgctg tcgccgttca acatcctctg gctggcgctg
     2521 cccccgatct ggggcctggg cttcatcgcg gcgctcaagt gggcgctgat gggcctgggc
     2581 atgggcctgc tattgcggcg attggggctt ggcatgcccg ccgccgtctt cggctccgtc
     2641 gcggggcagc tcatggggcc gatggtggcc tggttgcagt ggcagcacac cgaagccctg
     2701 gcctggatgc cctggctgct ccttgccgcg ctggcctggg tggacacccg ccgcctctta
     2761 tggctggccg cgttcggggc gctcgtggca gccgagctac tggccgggca cgtcgagacc
     2821 gccttctact cgttcgcgct gggcggggcg ttcgccctgg cggcgggggg cgctgccggc
     2881 atgcccccca ggcagaagtg gctctcgtta gccgggctgc tgggcgcggg cgtgctgggg
     2941 gccggtatcg cggccgccca attcctgccg ctgctggggg tgctcacctc tagctggcag
     3001 tgggtcgcga gggaaggctc caccacccac ctgacatcgc tgccccccga ggccgccctc
     3061 acctatctca gccccaacgg cttcggctcg gcaggctcat ggtacggccc gctcaactgg
     3121 atcgaaatca acggctacgt gggcgcgctc acgctcctgc tggcggtgtg gggcctggcg
     3181 gctgcaatct tcgcccgccg cgcgcccgac gcacaagccg accccacctt ccccgtgcgg
     3241 ctgggacagg ccctctcccc gcgcaagcaa ctgttctggg tgctcgccgc cataaccggc
     3301 atcagcatgg cctatggcat cccacccttc agcctcctgc gcaacctgcc cggcttcagc
     3361 agcagcctca actaccgcct catagccgta gccgccgcga gcctggtcgt cctgggcgcg
     3421 atgggcctcc aacggctcag cgagtatcga gtgccgagtg ccgagtaccg agtcgggcct
     3481 ttgccactca gaactcggta ctcggtactc ggcactttcc tcctcgccgg gctgggcctg
     3541 ctcttcctgg tggatggagt gcgggcgtgg tacgtcgata gcgcggctgc gggcgcgtac
     3601 atgcaggcgt ggaagatgtg ggcgggggcg ctgttctgcg cgggcggggc gctgatactg
     3661 gcccgcgtgt cggggctgct tggcacgcgg gcgttcgcgg cgcttgcggt cgtcctctta
     3721 ttgcttgacc tggggcgcgc caactggaac ttcaacgtta cgtcgcccct gggcacattc
     3781 tatcccgcca acgaaatgac cgatttcctg gcgcaacgcg gcatcaccga gagggtagct
     3841 atagacgccg cgtgggccga gccgaaccgg ttgatggcct accgcatccc cgacatccgc
     3901 tattacgacc ccaccatcga caatcgctac cgcgtcttca tccggctgat gagtcccggc
     3961 accttcacct cgccctatcc tgcatacccc atccacctga tgctcgacag gccgagcgcc
     4021 acgctgttgt cgatggtggg cgtcaagtgg ctggtaacta cctccaactt caacccgaac
     4081 acctggcagc ctgcgccatc gaagggtcca atctacgcgc cctggctgaa ccgcaacagc
     4141 ttcgtggcct gggagaaccg ctacgccaac ccctactcgt acctggcttc tcgcatccgg
     4201 gtggtgcccg acgaagcggc ggcccggcag cgtatgagcg aaatgaagct ggaggagatc
     4261 aacgaggcca ccgtggagca ccctgccggg ctgcccggcg aagtggcctc cgccagcaac
     4321 ggccagcccc tcaccgaagc cgaggtggac agcctggaga cggttgcgaa catccctggc
     4381 acgatacggg tcaggctgcg caccgagaag gcgcgcctgc tggtgatcaa cgaaagctgg
     4441 tcgccgggct ggcgggccac cgtggacggc accgaaacgc ccatctacag ggcgaactac
     4501 atcgtgcaag gcgtggtagt cccaccgggc gagcacgaag tggtgttcca ctacgacccg
     4561 cccatcttca agctggggct ggccgtcagc ggggtgtcgc tgctgggctg gttggggctg
     4621 ctcgcgctgg caatacggcg ctggcggcgc gctagggaaa tcctgcccgg tggttaaggg
     4681 tagcctgtag gggcaggccc ccgtgcctgc cctggtggta cgcgtacaat gttgctaatc
     4741 tccaacaagg gcaggcacgg ggaccggccc ctacatagca cccattcgtg cctgcccatt
     4801 acctgactaa ccagttaatc ttcatcaact gccaggcgac aggtgtagtg tgcacactga
     4861 tcagcaagcc acaaaccaca taagtgcctt ctggcgcatg ctacaccccc tcccctcgct
     4921 catgaccgtg gtggtggcgg gggcgtttgt ggtgcttgcc gccaaaggac tgccaccgct
     4981 ggggaggttc gtccaactgc tcgcggtcga gacg
//
LOCUS       DASRWZ010000728         7855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_607536, whole genome shotgun sequence.
ACCESSION   DASRWZ010000728 DASRWZ010000000
VERSION     DASRWZ010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7855
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607536"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VFH60_11500"
     CDS             <1..1155
                     /locus_tag="VFH60_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6314452.1"
                     /translation="ALFYFSFYGAAAAYLPFISIFYAHQGLSGSEIGVLAAIGPLMAL
                     LASPALAALADRKGWRRRLLVLSLAGTAVALLVVPLPTSFLWFLPVVALLSAVGSASI
                     PVADSLVARTAARRGLSYGKMRLWGSVSWAVVAAVGGALWEQAGFFTMFAVASLLFLA
                     TIPSAGWLEEDRASESQVRPPLRLVIGDVRLRVVLLATLGLGTAMSAMYTFSAIYIDR
                     IGGQSLVGLFAGVMAISELPVMHWSERITRRLGGPLTLVLAYSCFGVAYLGLAFVQSP
                     VLLLGIAFLQGLGFGLFLPTTVRLFADWAPAEWSSTSQGILSAGLWGLAPLIAGPLGG
                     IIYDNVGPIAVFFASAGVIALAALVIVLAQLASVFHLKTETGDAILKREA"
     gene            complement(1234..1566)
                     /locus_tag="VFH60_11505"
     CDS             complement(1234..1566)
                     /locus_tag="VFH60_11505"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET6314453.1"
                     /translation="MQAISRTLRAMVRAGMPQALELLHASQNHVGYSHAASPRDTICY
                     ICPMRDYVRLGFMYGGHLADPERVLEGTGKRLRHVKVRTLEATHGSALAQLVADAWVE
                     AEERTEKR"
     gene            complement(1640..3217)
                     /locus_tag="VFH60_11510"
     CDS             complement(1640..3217)
                     /locus_tag="VFH60_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6314454.1"
                     /translation="MPTRSARFVAVAFFLLISVLMPLGAAPRAAGAAATPKPAVRLTG
                     GEKCFSQTSRCMHGVFLGYWQDNGGVAQFGYPLTDELEEGGRTVQYTERARFELHEQN
                     RDTPYEVQLSLLGTQLVAGRTEAAFKRVSQPSSGTWFRESGHTLKEPFLSYWQRSGGL
                     PVFGFPVSEAFSEKSPTDGKTYTVQYFERNRLEHHPEAKGTPNEIQVGLLGREFYSRT
                     YGNTPPPPASPYPVSIDALQGMKRWGSDLRVERTTNETGAYTQYAITYRSGNLRISGQ
                     MYVPKGTGPFPVMIMNHGFIPIEDYVTGMDSRRESPFVASNGYVAIHPDFRNYATSDD
                     DENASQNLTAFGWADDSLNLVDAVKRSTLPYLDKTRIGYWGHSNGGQVSMMALVSQRQ
                     PDIKAFVLFAATSPNYAENFNRWTRPRTDQASSVRARHGFPEDNPAFYEGLSVGPHFG
                     EAVTKGPVLSFHGTGDTNTPFEWSQRSANLMEAAGIDITFVPLRGENHLFSDRAWRGG
                     VASQFLSFIDEHVKGVK"
     gene            complement(3334..3419)
                     /locus_tag="VFH60_11515"
     tRNA            complement(3334..3419)
                     /locus_tag="VFH60_11515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3384..3386),aa:Leu,seq:tag)
     gene            3759..4442
                     /gene="scpB"
                     /locus_tag="VFH60_11520"
     CDS             3759..4442
                     /gene="scpB"
                     /locus_tag="VFH60_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET6314455.1"
                     /translation="MTTDIDTAQQDTFAPGQQPPLTEREDIQEMAAALEALLFIAPEP
                     VPVHDLRKALQVRQPTLERVIEFLGKSLSEGKRGLRLQRHNDMLRLVTAPETALYIEK
                     LKGAQDTQRLSDAALETLALVAYTQPTTRPQIEAIRGVDCSGVVNTLMQRGLVVEVGR
                     LDTVGHPILYGTTLAFLQHFGLERPDQLPSVVGLPFRREERANGTTDVEGEQAATTPD
                     RSSEPPGPL"
     gene            complement(4402..4908)
                     /locus_tag="VFH60_11525"
     CDS             complement(4402..4908)
                     /locus_tag="VFH60_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6314456.1"
                     /translation="MSLILGNRLPDRAMKYLQVGRLVVLATVDEQGRPDTCPVSWVVA
                     LNPSVIRLAISREVNTYHNILNNEHVMISLVGGAMTLGIRGRARVLAESLDGIPLSMA
                     IIEMEVDEVKDDSVIGRGLQDELVKWEERRRSVSDWHVENALKTTGPLPQTDTSIMDQ
                     VAQNYDLA"
     gene            complement(5045..6289)
                     /locus_tag="VFH60_11530"
     CDS             complement(5045..6289)
                     /locus_tag="VFH60_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6314457.1"
                     /translation="MNIHSNGTTSSNGLPQRSVYLDHAATTSVHPDVLNAMLPYFTER
                     YGNPSSVHAWGRSAHQGLEGARRQVASVLGCRPREVVFTSGGTESDNFAMKGVAWAYR
                     RGLFPSKQPSTGPGHIVTSAIEHHAILHSAEELEKHGFEVTVLPVDQFGRVSLEAVAR
                     AMRPDTVLVSIMAANNEIGTIQPIAEIAAIAHEHGALFHTDAVQAGAYLDVDAAKLGA
                     DLLSLSAHKFYGPKGVGVLYIKANTPILSIQHGGSQEARRRASTENVPGIVGLGAALA
                     RTHQNLASEVSYVKGLRDRLVAGILQKVPDVHQMGHPTERLPNNANFCIEGVEGETML
                     LKLDMRGIGASSGAACASGSTEPSHVLRALGIPRELAQGSLRLTVGIDNSPEDIEYAL
                     DVLTACVAELRSGNRTMVGSEK"
     gene            6736..>7855
                     /locus_tag="VFH60_11535"
     CDS             6736..>7855
                     /locus_tag="VFH60_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314458.1"
                     /translation="MTDQVGNGLAEQKLPGSGCEEVRTEKDDGPSLGVLFVHGIGEQK
                     RGDTLPWAGDALFKWYRLWSCHGNIEVSSRAAPCEPYLTDTRLMRPVPSEMDAPPFSH
                     MKFQTTNQEVAQREQEWILAESWWASEFTPPTYAEMVVLGLSIAPWLIEQTGLRIIGD
                     ILRVVRDNNVLTTLTNQPRRGAPAGKPVDFLSRAFGWISDRLLDIAAVITWVMVGFLL
                     LTVGVLAQTLILALWVLALIPPLRDPVVKLQLGLTGWLGDAYLMATSPIRFSAMVSQV
                     RRDVQWLASKQAGKGCCKTVAVIAHSGGAVVAHEALTGPGAPQIGQDILLITYGSGQS
                     KVDTIAKLRNDRTPFFRFSPVFRAAGVLFALAALYLGVQ"
BASE COUNT         1377 a         2424 c         2464 g         1590 t
ORIGIN      
        1 gcgctattct acttcagctt ctacggggcg gccgccgcat acctgccttt tatcagcatc
       61 ttctacgctc accagggcct ctccggcagc gagattggcg tgctggctgc tatcgggccg
      121 ctgatggcgt tgctggcctc acccgctctt gcggcgctgg ccgaccggaa gggctggcga
      181 cggcgcttgt tggtgttgtc gttggcgggc actgccgtgg ccctcctggt cgtgccgttg
      241 cccacctcct ttttgtggtt cctgccggtt gtcgcgctct tgtcggcggt gggtagcgct
      301 tccatcccgg tggcagacag cctcgtggca cgcacggcag cgcgcagggg cctctcctac
      361 ggaaagatgc ggctgtgggg gtccgttagc tgggccgtag tggcggcggt ggggggcgcg
      421 ctgtgggagc aggcggggtt cttcacgatg ttcgcggtgg caagcctgct gttcctggcg
      481 accattccct ccgccgggtg gctggaggaa gaccgggcgt ccgaatcgca ggtcaggccc
      541 ccgctacggc ttgtcatcgg cgacgtcagg ctacgcgtag tgctccttgc cacgttgggg
      601 ctgggcaccg ccatgtcggc tatgtacacg ttctcggcga tctacatcga ccgcataggc
      661 gggcaatcgc tggtgggcct gttcgccggg gtgatggcga taagcgagct gcccgtgatg
      721 cactggagcg agcgtatcac gcgccgcttg ggcgggccgc tgacgctggt gctggcctac
      781 tcatgcttcg gcgtcgcgta cttggggctt gccttcgtac agtcgcctgt gctgctcctg
      841 ggcatcgcct tcctacaggg gttagggttc gggctgttcc tccccaccac cgtgcgcctc
      901 ttcgccgact gggcccccgc cgagtggtcc tccacctcgc agggtatcct gagcgcggga
      961 ctctggggtc tagcgccgct gatagcaggc ccgcttgggg gcataatcta cgataacgtc
     1021 ggccccatag ccgttttctt tgccagcgcg ggggtaatag ccctggcagc cctcgtcatc
     1081 gtcctggctc agcttgccag tgtattccat ctgaagacgg aaacgggaga tgcgattctg
     1141 aaacgcgaag cgtgacgctt ggtagaatac aagcaaaaag aaggccgcct ggtgtttgcc
     1201 gggcgacctt ctttttaagg gctgtagagc tattcagcgt ttctcggtgc gctcctcggc
     1261 ttcgacccag gcgtccgcta ccaattgtgc cagggcagac ccgtgcgtag cctcaagcgt
     1321 cctcaccttg acgtggcgca ggcgtttgcc cgtgccttcc agcacccgct ccgggtcggc
     1381 caggtgcccg ccgtacatga agccaaggcg tacgtagtcc ctcatggggc agatatagca
     1441 gatggtgtcg cgaggtgacg cggcatgtga gtagccaacg tggttctggc tcgcatgcaa
     1501 tagctcgagg gcttgaggca tgcccgccct caccatagcc cgcaaagtcc ggctgatggc
     1561 ctgcacttcg gggggtagcc cgcgaagaag tcctcaacca ggtcgggcat gcgtgctcct
     1621 tcccttaccc acgaccggcc tacttaacac ccttcacgtg ctcgtcgatg aacgacagga
     1681 actgcgaagc gacaccgccc cgccaggcac ggtcgctgaa caggtggttt tcgcccctta
     1741 gcggtacgaa cgtgatgtcg atgcccgccg cttccatgag gttcgcggag cgttgcgacc
     1801 actcgaacgg cgtgttcgtg tcgcccgtgc cgtggaacga gagcaccggc cccttggtga
     1861 ctgcctcccc gaagtgcggc ccaaccgaca gcccctcgta gaaggccggg ttgtcctccg
     1921 ggaaaccgtg tcttgccctg acgctgctcg cctggtcggt gcgcgggcgc gtccagcggt
     1981 tgaagttctc cgcgtaattg ggggacgtgg cggcgaagag cacgaacgcc ttgatgtcgg
     2041 gctgccgttg cgacaccagc gccatcatgc tcacctgccc gccattggaa tgcccccagt
     2101 agccgatacg ggtcttgtcg aggtagggca gggtggagcg tttcaccgcg tccaccaggt
     2161 tgagcgagtc gtcggcccag ccgaaggccg tgaggttctg gctggcgttc tcgtcgtcgt
     2221 cggaggttgc gtagttgcgg aagtcagggt ggatggctac gtagccgttg gaggccacga
     2281 agggcgactc cctgcgcgag tccatgccgg tgacgtaatc ctcaatcggg atgaagccgt
     2341 ggttcatgat catcaccggg aacggccccg tgcccttcgg cacgtacatc tgcccgctga
     2401 tgcgcaggtt gccgctcctg tacgtgatcg cgtactgggt gtacgccccg gtctcgttcg
     2461 tggtgcgctc cacccgcaaa tcgctgcccc accgcttcat cccctgcaag gcgtcaatcg
     2521 acacggggta aggggaagcc gggggcggcg gggtgttgcc gtaggtgcgg ctgtagaact
     2581 cgcggccgag caggccaacc tgtatctcat tcggcgtgcc tttagcctca gggtggtgtt
     2641 cgaggcggtt gcgctcgaaa tactgcaccg tgtaggtctt gccgtcggtg gggcttttct
     2701 cgctgaatgc ctcggatacc gggaagccga acacgggcag gcccccgctg cgctgccagt
     2761 aagagaggaa cggctccttg agcgtgtgcc cgctctccct gaaccaggtg ccgctcgagg
     2821 gctggcttac gcgcttgaaa gcggcctccg tgcgcccggc caccaactgc gtgcccagca
     2881 ggcttagctg cacctcgtag ggggtgtcgc ggttctgctc gtgcagttcg aagcgggcgc
     2941 gctcggtgta ctgcacggtg cgtccgccct cctccagctc gtcggtgagg gggtagccga
     3001 actgggccac gccgccgttg tcctgccagt agccgagaaa gacgccgtgc atgcagcgcg
     3061 aggtctgcga gaagcatttt tcgccgcccg tcagccgcac cgccggtttg ggggtggcag
     3121 ccgctcccgc ggcccgtggt gccgcgccga ggggcatcag taccgaaatg agaaggaaaa
     3181 aggcaaccgc aacgaagcgc gccgatcttg ttggcataat cgtgctccaa actggtcttt
     3241 atagttgtac gccgccacgg tctaagtgga gtatacgggg aaggtttcgg ataaagcaaa
     3301 aggacgcctg ggggagcgtc ccaaaggttc gattggtgcg ccagaaggga ttcgaaccct
     3361 tacgcccttg cgggcactag ctcctaaggc tagcgcgtct gccattccgc cactgacgcg
     3421 ttttgtaagg ttaattatac cgattcggac aggtcgagac aagatgtttg tgcctgggtc
     3481 tcatggctcc tttgcattgg gaatgaggac caacccacgc ccttgtcatt ctgagccgag
     3541 cgaagaatct cgtcccacag cacacaaatg ccacctttct ttatgccagt atattcaccg
     3601 aggaaaaagg gcacatccag gtgtaggacg agattcttcg ctccgctcag aatgacaaag
     3661 accaccaggg ttcttgtatc ggatattgac acagccgcag cttgatccct aatgagtaga
     3721 acgtgacgcc caccccgcca cttacttata atatgtccat gacaaccgac atcgacaccg
     3781 cgcagcagga caccttcgcc cccggccaac agccgcccct caccgagcgc gaggacatcc
     3841 aggagatggc agccgccctg gaggccctgc tcttcatcgc gcccgaaccc gtgccagtgc
     3901 acgacctgcg caaggcgttg caggtccggc agccgacgtt ggagcgggta atagagttcc
     3961 tggggaagag cctttccgag gggaagcggg gactgaggct tcagcgtcac aacgatatgc
     4021 tcaggctggt tacggccccc gagaccgccc tctacatcga gaaactgaag ggggcacaag
     4081 acacgcagag gctttcggac gccgcgctcg aaacgctggc cctcgtcgct tacacgcagc
     4141 ccacgacccg gccccagata gaggccatac ggggtgtgga ctgtagcggc gtggtgaata
     4201 cgctcatgca gcgcggcctc gtggtggagg tgggcaggct ggacacggtg ggccacccca
     4261 tcctctacgg cactacgctg gccttcttac aacacttcgg gttggagcgg cccgatcaac
     4321 tgccttcggt cgtgggcctg ccgttccggc gtgaggaaag ggcgaacggc acgaccgacg
     4381 tagaagggga gcaagcggcc actacgccag atcgtagttc tgagccacct ggtccattat
     4441 agaagtgtcc gtctgaggga gaggcccggt ggtcttgagg gcgttctcca cgtgccaatc
     4501 ggacacggag cggcggcgct cctcccactt gaccagctcg tcctgaaggc cccggcctat
     4561 gaccgagtcg tccttcacct cgtccacttc catctcgatg atagccatcg acaacgggat
     4621 gccatccagg ctttccgcca acacccgcgc tcggcccctg atgcccagcg tcatcgcccc
     4681 gcccacgagg gagatcatca cgtgctcgtt gttgaggatg ttgtggtagg tgttgacctc
     4741 gcgggagatc gccaggcgta tcaccgaggg gttgagcgcc actacccacg acacgggaca
     4801 cgtatcgggg cggccctgct cgtcaaccgt agccagcacc accagcctgc ccacttgcag
     4861 gtacttcatc gcccggtcag gtaaacggtt gccaagtatc aggctcatgt ggggcctcct
     4921 tgggaagtga tgagtaatga ggactgagga ctgagtaaag agacgccagc acaaataatg
     4981 cgctaagtgg ctgagggttt cggcctgtgc ctctcaaccc tcagtcctca ttactcatta
     5041 ctcattactt ctcagacccc accatcgtcc tgttgccgct tctaagctct gccacgcagg
     5101 ctgtcagtac gtcgagggcg tattctatgt cctccgggga gttgtcgatg cccaccgtga
     5161 ggcggaggct gccctgggcc agttcccttg gtatgccgag ggcgcggagc acgtggctcg
     5221 gctcggtgga accggaggcg caggccgctc cactcgaggc ccctatgccg cgcatgtcca
     5281 gcttcagcag catcgtctcg ccctcgacac cctctatgca gaagttggcg ttgttcggca
     5341 ggcgctcggt ggggtgcccc atctggtgca cgtccggcac cttctgcaag attcctgcca
     5401 caagccggtc gcgcagcccc tttacgtaag agacctcgct cgccaggttc tggtgcgtcc
     5461 tcgccagcgc cgccccaagc cccacaatgc ccggcacgtt ctcggtggac gcccggcgtc
     5521 gcgcctcctg cgagccgccg tgctgtatcg acaaaatcgg cgtgttggcc ttgatgtaga
     5581 gcacgcccac accttttggc ccgtagaact tgtgggccga cagcgaaagc aggtcggcgc
     5641 ccagcttcgc cgcatcgacg tccaggtaag cccccgcctg tacagcgtca gtgtggaaca
     5701 gggcaccatg ctcgtgcgcg atggccgcta tctccgcgat gggctgtatc gtgccgatct
     5761 cgttgttggc cgccatgatg ctcaccagca ctgtatcggg gcgcatggcg cgcgccaccg
     5821 cctcaaggga cacacgcccg aactggtcca ccggcagcac cgtcacctcg aacccgtgct
     5881 tctcaagctc ttcggcggaa tgcagtatgg cgtggtgctc gatagccgat gtgacgatgt
     5941 gacccggccc ggtcgaaggc tgcttgctgg ggaagaggcc gcgccggtaa gcccaggcca
     6001 cgcccttcat cgcgaagttg tccgactcgg tgccgcccga cgtaaagacg acctctcgcg
     6061 ggcggcagcc tagcacggag gccacctgcc tgcgcgcccc ctccagtccc tggtgcgccg
     6121 acctacccca ggcgtgtacg ctcgacgggt tgccgtatcg ctcggtaaag taaggcagca
     6181 tggcgttcag cacgtcgggg tgaacgctcg tggtggccgc gtggtccagg tatacggacc
     6241 tttgcggcag gccgttgctc gatgtggtgc cgttgctatg tatgttcatg ttatcttcgc
     6301 tgctcctatt tattaccgcc tgtaccgggt ttgcttttgc ctgtgtatcg atgcggatat
     6361 tgcgcacttt aatgattata ccactcggct tgtagcatta gaaccccacg attgggttag
     6421 gaaggctgta gtcagccata gcacgggaag ctggtagctg ccccgcaggg acgccctggc
     6481 aaccaggccc tgtggcggta gtcccaccgt gaatgtctgc accctgcaag ctatatatca
     6541 cctggcgtcg ctacaaagcc acctgcttct ccttagacca cgtaccgcag ttttaccgtt
     6601 ctgccgtttt gccatactgt gacacttatt gtgacgctta gtatcgcacg attctcgcta
     6661 tattgtgctt gggtagacga ctactaaccc gctagagagt gcaatcaggt tgagatagga
     6721 gggaaggagc cagagatgac cgaccaagtt ggtaacgggc tagctgagca gaagctaccg
     6781 ggcagcggat gtgaggaagt gcggaccgag aaagatgacg gccccagcct tggggtgctg
     6841 ttcgttcacg gtatcgggga gcagaaacga ggcgataccc taccctgggc gggagacgcg
     6901 ctattcaagt ggtaccggct ctggtcctgc catggcaata tcgaggtcag ttcccgtgcc
     6961 gccccgtgcg agccgtactt gaccgatacc cgcttgatgc ggcctgtgcc cagcgagatg
     7021 gacgctccgc ctttttcgca catgaagttc cagacgacca atcaagaggt ggcgcagcgc
     7081 gagcaggaat ggatactcgc ggagtcgtgg tgggcctccg agtttactcc acctacctac
     7141 gctgagatgg tggtcctggg gctgagcatc gcaccctggc tgatagagca gaccggcctc
     7201 aggatcattg gtgacatact cagagtggta agagacaata acgtcctcac cacactgaca
     7261 aatcagccgc gacgtggtgc gcctgcgggc aagcctgttg acttcctcag cagggcattc
     7321 ggttggataa gcgaccgcct tctcgacatc gctgcggtaa tcacgtgggt gatggtcggc
     7381 ttcttgttgc tgactgttgg ggtactggct cagacgctaa tactggcctt gtgggtcctg
     7441 gcgctcatac cgccgttgcg cgatcccgtg gtcaagctcc agctagggct tacgggctgg
     7501 ttgggcgacg cgtacctgat ggctaccagc ccgattcgat tcagtgcaat ggtcagccag
     7561 gtccggcgcg acgttcaatg gctggcttca aaacaagccg gcaagggctg ctgcaagaca
     7621 gtggccgtga tagcacactc cggcggcgca gtggtagccc acgaagccct gacggggccg
     7681 ggcgctcctc agatcggcca ggatattctg ctcatcacgt acggatcggg acagagcaag
     7741 gtggacacca ttgccaagct aaggaacgac aggaccccct tctttaggtt cagccccgtc
     7801 tttcgcgctg cgggggtatt gtttgcgctc gcagccctgt acctgggagt gcaaa
//
LOCUS       DASRWZ010000729         8858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_949557, whole genome shotgun sequence.
ACCESSION   DASRWZ010000729 DASRWZ010000000
VERSION     DASRWZ010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949557"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1203)
                     /locus_tag="VFH60_11540"
     CDS             complement(127..1203)
                     /locus_tag="VFH60_11540"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET6314459.1"
                     /translation="MSNVEDSVVGRGAISDRPLDERVPAWTMPQYSRINEWLEGAGRI
                     WLPLHVSPDGDCLGSSLAFAKALKLRGFDCVVVSSDPIPDMYAGLYNPDDVFIGAVPP
                     GPPATHIACLDISDPRRTGDFYAANEATLRGETGVKVLNIDHHATNTQFGDLQLLDTA
                     APACAEQIAVALNELEWAVDAEIARYILLGIITDTLGFRTPATTARTLRVSAHMLERG
                     GNLQVVIDAVFNSRPLSAVLLWGKVLNGVTCAVDGRVVYAVVTPRMLDETGAKEEDLE
                     GLSSYLATVKGDVKVAAVLKEREDGTTRVSFRSQPGTDVAAIARHFGGGGHPQAAGAT
                     IQAVGDEAERQFLEACEIGLDAQA"
     gene            complement(1184..1675)
                     /gene="rbfA"
                     /locus_tag="VFH60_11545"
     CDS             complement(1184..1675)
                     /gene="rbfA"
                     /locus_tag="VFH60_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET6314460.1"
                     /translation="MTRRQIQVGDEIKQIVSVLLQRELKDPRIGFVTVISVQVTQDLK
                     YARIHVSVMGSAEEQRATMDALTSARGFIRREIAARMEIRFVPEIQFRLDKGAEYSDQ
                     IARLLNELKESDAEKVVGGFGEDPGHVSEAEEADSAGDTAATGQTEEDGEDGEEEDEQ
                     RRG"
     gene            complement(1792..3579)
                     /gene="infB"
                     /locus_tag="VFH60_11550"
     CDS             complement(1792..3579)
                     /gene="infB"
                     /locus_tag="VFH60_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET6314461.1"
                     /translation="MGATLERPVRRAALPPYMTVKELADELNVKPVEVIKEMMKHGVM
                     ATINQQIDYDTGAIVAVGLGFEVSEAIPEIEEDLGEVMVAQQEAADNSRVMPRPPVVT
                     IMGHVDHGKTKLLDSIRKANVIATEAGGITQHIGAYQVELQGRKITFLDTPGHEAFTA
                     MRARGAQATDIVILVVAADDGVMPQTVEALSHAKAAGVPIVVAINKIDKEDANPDRAM
                     TQLSEHGLVPTQWGGDTEFIPVSARTGDGIPTLLETILLVAEIAEFRANPERRATGVI
                     IEAEMDRNRGPIATVLIQNGTLNLRDYIVAGATWGRVRAMQDDKGRKLRKAEPSTPAE
                     ILGLVAVPMAGDTIISAPDETTAKQIAEQRARQKRLEEGSQGIKAISLDDLFSQIQAG
                     RVKELRIILKADVQGSLEAITQTLNKLTNEAVKVNVLHRATGGITESDVSLAYASGAI
                     IIGFNVRPDAAAKRAAEKSNIDLRFYDIIYNLSEDIEKAMRGLLDPTFRDVTDGYAEV
                     RAVFRLPRNEQAAGLIVTEGKITRNGQVRLLRNGAVIYDGTVSSLKRFKDDVREVAQG
                     YECGIGLNNFHDFQEGDTLEFYRKEQVRA"
     gene            complement(3606..3818)
                     /locus_tag="VFH60_11555"
     CDS             complement(3606..3818)
                     /locus_tag="VFH60_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314462.1"
                     /translation="MAAGVAGRVVAQAVPGRRVVALRDRAVRAGRVPRAVIAALAALT
                     GAARIEAAVSAGAPTTARTAAARLAR"
     gene            complement(4029..4337)
                     /locus_tag="VFH60_11560"
     CDS             complement(4029..4337)
                     /locus_tag="VFH60_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET6314463.1"
                     /translation="MAKPTKGPRPKPVPQRTCVACKTTGPKRGLVRLVRLPDGHVEVD
                     ETGKKSGRGAYLCRTRSHWEKALQSRALEYALKTAIGIDDKAALQVYAAGLPEEDSEE
                     "
     gene            complement(4417..5823)
                     /gene="nusA"
                     /locus_tag="VFH60_11565"
     CDS             complement(4417..5823)
                     /gene="nusA"
                     /locus_tag="VFH60_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET6314464.1"
                     /translation="MKSDLYAAINQIAAERNIPREAILRGIEDAIITAYRRISGTDQN
                     VAVKLDLQSGDARVFERRTIVEDVTDPALEVGLDEVLEVEPNAKVGDMIEKETTPANF
                     GRIAAQTAKQVVMQRIREAERDRVFSEFNDREGDIVLANVQRQDNNGNWILEVGRAEA
                     ILPPNHQVKTERYRSGNRMRVLLLEVQKNPKGPQLIVSRADRSFLKRLFELEVPEIHS
                     GIVEIKSIARESGMRSKVAVAARQSNVDPVGSCVGMKGQRIQKIVDELGGEKIDVVLW
                     DADMSVYIANALSPAQVLSVDLSEPEKTATVVVPERMLSLAIGKEGQNARLAAKLTGW
                     RIDIKSAAIPEEDQEEYVPDDEELARRAAVAMRAEHAANAAAAADGNNGFQPPVAGEN
                     GAGPKEERRKVRPNNTVVYQNVAYGPLPEAMPGETIRLRDLGDNVEIRTDSGRTIATY
                     PVQAGEPAEEAEAEEAEA"
     gene            complement(6011..8371)
                     /locus_tag="VFH60_11570"
     CDS             complement(6011..8371)
                     /locus_tag="VFH60_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314465.1"
                     /translation="MSSVSNSAPQAPDAAGGVTPTPGEATASMRVHLLALLGYLALAV
                     LVTYPAITQFGTNVPGDLLADRDQNLWNLWWVREAIAHATNPYHTGMLYYPYGVDLYY
                     HTLGLPLGLIGLLPQLLFGLPAAYNTVLLAAFTLSGYGAFRLALRFVSTGEGWPLPPW
                     AVAFVAFVGGVVFAFTPYTLDALKGQLEVLSLQWMPFYAEAWVMAWRTGRLRYGLPAG
                     VFFALAALSSLYYAAYLAIFSLAYVVYQLWARRGARGKEAGRGWGGLVPPLVVAPIAA
                     LVLTLPLLVGLVRDRDNPRLAVEAGVEHRLAHSADLLSFVAPPHDHPLLGWDPADRPG
                     VNEPPIHDYLSLGFAALTLSVLGGIVGWRTSRGKFWVGLALFALVLSMGPQLQVGRMQ
                     TGVPLPFRLLEWLPGMEAIAKPERLVVLARLCMGVLAAWGVGWLLSRLVLARPAAWGR
                     TVAACAVVLALLLVELPIHPRYVEPLDVPGGFRTLAGQGGGALMELPFATQQSEVGGE
                     RMLYQTAHGRPIMGGYLARTYNSPITDSCGPFWSFISARYLPVEGRDIVSPTMTSRPL
                     DILAYYDIGYVALYNNYNGPGTTPLDPRERAAFEDILSHVSHAPPLYADDYVSLHKVD
                     TYPRENIQPALIVSDGWYEAEDSGGEPFRWVRGDFGRLCIFTPGPLRAALSMQVTAFD
                     SDQEVTIYQSTARPGQTIKVGQSAGQNLPIITSPIVGEPIFRGTIGTTGSVVIVELEL
                     PAGMTEVRIVPEGGGVTPQSLDPSVKDDRALTVGFKRVRLEPVAPK"
BASE COUNT         1502 a         2993 c         2670 g         1693 t
ORIGIN      
        1 tggtgcttga gcaagaagag tcaagaacgg gcggctgatc cttcgctacg ctcaggatga
       61 caagaggagg gcatgtccca tgaaggtaaa aggctggcgc tcacgcactc tgttcagtaa
      121 gccgcatcac gcttgggcgt cgaggccgat ctcgcaggct tccaggaact gcctttcggc
      181 ctcgtccccc accgcctgga tggtcgcgcc cgcggcctgg gggtggccac cgcctccaaa
      241 gtgacgggct atagctgcca cgtcggtgcc cggctggctg cggaaggaca cccgcgtggt
      301 gccgtcctcg cgctccttga gcacggcggc gaccttcacg tcacccttca cagtggcaag
      361 gtagctggaa agcccctcca ggtcttcttc cttcgcgcca gtctcgtcca gcatccgggg
      421 cgtcaccacc gcgtagacga cccgcccgtc caccgcgcac gtcaccccgt tgagcacctt
      481 gccccacaac aagaccgccg acagcgggcg gctgttgaac accgcgtcta ttaccacttg
      541 caggttgccc ccacgctcca acatgtgggc cgacacgcgc aaggtgcgag cggtggtggc
      601 gggggtgcga aaaccaagcg tgtcggtgat gatgcccagc agtatatagc gggcgatctc
      661 cgcatcgacc gcccactcaa gctcattgag ggccacggct atctgctcgg cgcacgccgg
      721 ggcggcggta tcgagcagtt gcaggtcgcc aaactgggtg ttggtggcgt ggtggtcgat
      781 gttgagcact ttcacgcctg tctcgccccg cagggtggcc tcgttggcgg catagaagtc
      841 gcccgtgcgc ctggggtcgc tgatgtcgag gcaggcgatg tgagtggcgg ggggtccggg
      901 gggcacggcc ccgataaata cgtcgtcggg gttgtacagc ccggcgtaca tgtcgggtat
      961 ggggtcggag gagaccacca cgcagtcgaa gccgcgcagc ttgagggctt tggcgaaggc
     1021 gagggaagag ccgaggcagt cgccgtcggg cgaaacgtgg aggggcagcc agatgcgccc
     1081 ggcgccttcc aaccactcgt tgatgcgcga gtactggggc atcgtccagg cgggcacgcg
     1141 ctcgtcaagc ggccggtcgc tgatagcccc acgccccacg acgctatcct ctacgttgct
     1201 catcctcctc ctctccgtcc tctccgtcct cttcggtctg ccccgtggcg gcggtatccc
     1261 cggcgctatc tgcttcctcc gcctcgctca cgtggccagg gtcctccccg aagccgccaa
     1321 ccaccttctc ggcgtccgac tccttcagct cgttgagcag ccgcgcaatc tggtcgctgt
     1381 actcggcccc cttgtccagc ctgaactgga tttcgggcac gaagcgtatc tccatgcgcg
     1441 ctgcaatctc gcgccggata aacccccgcg cgctcgtcag cgcatccata gtagcccgct
     1501 gctcctcagc tgaccccatg acgctaacgt gaatacgcgc gtacttcaag tcctgcgtca
     1561 cctgcacgct aatgaccgtc acgaacccaa tccgcgggtc cttcaactca cgctggagca
     1621 gcaccgacac gatctgcttt atctcatccc caacttgtat ctgccgtcga gtcataataa
     1681 ttcttaattc ttaattctta atgtttaatt gttagctgct cggcaggcca ccgcttcggt
     1741 gacgagcctc cgcaattaag aattaaacat taagaattaa gaattcgcac gctaggcgcg
     1801 cacctgctcc ttgcggtaga actccagggt gtcgccttcc tggaagtcgt ggaagttgtt
     1861 gaggccgatg ccgcactcgt agccctgggc cacttcgcgc acgtcgtcct tgaagcgctt
     1921 gaggctggag acggtgccgt cgtagatgac cgccccgttt cgtagcaggc gcacctggcc
     1981 gttgcgggtt atcttgccct cggtgacgat aagccctgcg gcctgctcgt tgcggggcag
     2041 gcggaacacc gctcgcacct cggcgtaacc gtcggtcacg tcgcggaagg tcgggtccag
     2101 caggccccgc atggccttct cgatatcttc cgacaggttg tagatgatgt cgtagaagcg
     2161 taggtcgatg ttgctcttct cggcggcgcg cttggctgcc gcgtcggggc gcacgttgaa
     2221 gccgatgatg atggcccccg aagcgtaggc tagcgacacg tcagactccg tgatgccgcc
     2281 ggtggcccgg tgcaacacgt tcaccttgac cgcctcgttg gtcagcttgt tgagcgtctg
     2341 cgtgatagcc tccaacgaac cttgcacgtc ggccttgagg atgatgcgta gctccttcac
     2401 gcgcccggcc tgtatctgcg agaacaggtc gtccaggctg atggccttga tgccctgcga
     2461 gccttcctcg agccgcttct ggcgggcgcg ctgctcggct atctgcttgg cggtcgtctc
     2521 gtcgggcgcg ctgatgatcg tatcgcccgc catcggcacg gccaccaggc ccaggatctc
     2581 ggctggggtc gaaggctcgg ctttgcgcag cttgcgcccc ttgtcgtcct gcatggccct
     2641 tacgcggccc caggtcgccc cggccacaat gtagtcccgc aggttgagcg tgccgttctg
     2701 gatcagcacc gtagcgatgg ggccgcggtt gcggtccatc tcggcctcga taatcacgcc
     2761 cgtggcccgc cgctcggggt tggccctgaa ctccgcgatt tcggccacca gcaggatcgt
     2821 ctccagcaga gtggggatgc catcgcccgt gcgcgccgac accgggatga actcggtatc
     2881 gccaccccac tgcgtcggca ccaggccgtg ctccgaaagc tgcgtcatgg cgcggtcggg
     2941 gttagcgtct tccttatcga tcttgttgat ggctaccacg ataggcacac cggcggcctt
     3001 ggcgtgcgaa agcgcctcca ccgtctgcgg catcacaccg tcgtcggcgg ccaccacgag
     3061 gatgactatg tcggtcgcct gggcgccacg cgcgcgcatg gcggtgaacg cctcgtgccc
     3121 cggcgtgtcc aggaaggtga ttttgcggcc ctgtagctcc acctggtacg cgcctatgtg
     3181 ctgggtgata ccgcccgcct cggtcgcgat aacgttggcc ttgcgtatcg agtcgagcag
     3241 cttggtcttg ccgtggtcta cgtggcccat gatggtcacg accggcgggc ggggcatgac
     3301 gcggctgttg tccgccgcct cctgttgcgc caccatgacc tcacccaggt cctcctcgat
     3361 ttcggggatg gcctcgctta cctcgaagcc caggcccacc gccacgatag ccccggtgtc
     3421 gtagtctatc tgctggttga tcgtagccat gacgccgtgc ttcatcatct ctttgatgac
     3481 ttccaccggc ttgacgttca gttcgtcggc taattccttc acagtcatat agggaggcag
     3541 ggctgcccgg cgcacgggcc gctccaacgt agcgcccaag cgagcgcctc cgccgaagcc
     3601 gcgccctacc gcgcgagacg ggccgctgcc gtgcgcgccg ttgttggagc gcccgccgaa
     3661 accgccgcct ctattcgggc cgcccctgtt agggccgcca gggccgcgat aaccgcccgc
     3721 gggacgcgcc ccgcccggac cgcccggtct cgcagcgcca ccacccgccg gcctgggacc
     3781 gcctgcgcca ccacccggcc tgccacgccc gccgccaccg cgagggcgcg gtccgcctcc
     3841 gggtcctcgt gacgtactct cttcctctac gggtccgtcc gctccgtcat cattcccccc
     3901 atgcacaggg ggcactatca cataatctat cttctttggc atccattacc tccttggtcc
     3961 ctaccgagcg acgagcgacg agtgactcta tctactcaca atccacacaa cttgctcgct
     4021 actcgtccct actcctcgct atcctcttcc ggcagaccgg cagcgtatac ctgcaatgcc
     4081 gctttatcat caatacctat agctgttttt agcgcgtatt ccagcgccct cgactgcaag
     4141 gctttctccc agtgagaccg cgtcctgcac aggtaagccc cgcgtcccga cttcttccct
     4201 gtctcgtcca cctctacgtg cccgtccggc agccgcacca gacgcaccaa ccccctctta
     4261 ggcccggtgg tcttgcatgc cacgcaggtg cgctgcggta ccggcttggg ccgaggcccc
     4321 ttggttggct tcgccatttc taatctcacc ttagaaggag taggtgggaa agtgggggca
     4381 ccccacaccc cgtcaggggg agacccctgc acccctctac gcctccgctt cctctgcttc
     4441 tgcttcctcc gccggttcgc ctgcctgcac cgggtacgtg gctatcgtgc ggcccgagtc
     4501 ggtgcggatt tctacgttgt cgcccaggtc gcgcaggcgg atggtttcgc cgggcatcgc
     4561 ttcgggcagg gggccgtagg ctacgttctg gtagacgacc gtgttgttgg ggcgcacctt
     4621 gcggcgctcc tccttagggc ctgcgccgtt ctcgcctgct acgggcggct ggaagccgtt
     4681 gttgccatcg gcagcggcag ccgcgttggc ggcgtgctcg gcgcgcatgg cgacggcggc
     4741 gcggcgggca agctcctcgt cgtcaggcac gtactcttcc tggtcctctt cgggtatcgc
     4801 cgcgctcttg atgtcgatac gccagccggt gagcttggcg gccaggcggg cgttctgccc
     4861 ctccttgccg attgccagcg acagcatcct ctcaggcact actaccgtgg ccgtcttctc
     4921 aggctcgctc aggtccaccg acagcacctg cgcgggcgag agggcgttgg cgatgtacac
     4981 cgacatatcg gcgtcccaca gcaccacgtc gatcttctcg ccgccaagct cgtccacgat
     5041 cttctggatg cgctgtccct tcatgccgac gcacgagcct acagggtcca cgttgctctg
     5101 gcgggcggcc accgccacct tggagcgcat gcccgactcg cgcgcgatgc tctttatctc
     5161 cactatgccg ctgtgtatct ccggcacctc aagctcgaac aaccgcttga ggaacgacct
     5221 gtcggccctt gacacgatca attgcggtcc tttcgggttc ttctgcactt cgaggagcag
     5281 cacccgcatg cggttgcccg agcggtagcg ctccgtcttc acctggtggt tgggcggcaa
     5341 aatcgcctcg gcccggccca cctccagtat ccagttgccg ttgttgtcct gccgctgcac
     5401 gttggcgagc acgatgtcgc cctctcggtc gttgaactcg ctgaatacac ggtcgcgctc
     5461 ggcctccctg atgcgctgca tgaccacctg cttggctgtc tgcgcggcga tgcgcccgaa
     5521 gttggcgggg gtagtctctt tctcgatcat gtcgccgacc ttggcgttcg gctcgacctc
     5581 gagcacctcg tccagcccca cctccagcgc ggggtccgtt acgtcttcca cgattgtgcg
     5641 gcgctcgaat acgcgagcat cgcccgactg caagtcgagc ttcacagcca cgttctggtc
     5701 cgtgcccgaa atacgcctat aagccgttat gatggcatcc tcgatgcccc gcagtatcgc
     5761 ctcacgcggt atgttgcgct cggcggctat ctggttgata gctgcatata agtcactctt
     5821 catttgttgc taaccccctg tattcagttt tagattttgg attttagatt ttggatttgc
     5881 ggggcttgga aggacaatcc aaaacccaaa atccaaaatc taaaatggtc ccgttcccta
     5941 atacgacaaa aaaagtgggg acctcccact ttcgcaagtc cggcacgcgt gttgtagcta
     6001 cctaattata ctactttggg gccacaggtt caagacgaac gcgcttgaat cctacggtca
     6061 atgcgcggtc gtccttgacg ctcgggtcca gcgattgcgg ggtcacgccc cctccttccg
     6121 gcacgattcg tacctccgtc atgcccgcgg gcagctcaag ctctactata actacagagc
     6181 cggtggtgcc gattgtaccc ctgaatattg gctcacctac tattggagac gtgataatgg
     6241 ggaggttctg tcccgcactt tgtcctacct ttatagtttg tcccggtcgc gccgttgatt
     6301 ggtaaatagt gacctcctgg tcgctgtcga aagcagtgac ctgcatggat aacgcggcgc
     6361 gtagcggccc aggtgtgaag atacatagcc ttccaaaatc gccccgtacc caccggaacg
     6421 gctctccccc cgaatcctct gcttcgtacc agccgtcgct cacaatcagg gccggctgaa
     6481 tgttctctcg tgggtaggta tctaccttgt gcaagctcac gtaatcgtcc gcgtagaggg
     6541 gcggggcgtg cgagacgtgg gagaggatgt cctcgaaggc cgcgcgctcc cgcgggtcga
     6601 ggggcgtagt gccggggccg ttgtagttgt tgtacagggc gacgtagcct atgtcgtagt
     6661 aggccaggat gtcaagcggg cgagaggtca tggtgggcga gacgatgtcg cgcccctcca
     6721 cgggcaggta gcgggccgag atgaagctcc agaaggggcc gcacgagtcg gtgatggggc
     6781 tgttgtaggt gcgggccagg tagccgccca tgataggtcg tccgtgcgcc gtctggtaca
     6841 gcatccgctc gccgcccacc tcgctctgct gcgtggcgaa cggcaactcc atcagcgcgc
     6901 cgccaccctg ccctgcaagc gtgcggaagc cccccggcac gtccagcggc tccacgtagc
     6961 gcgggtggat gggcaactcc accaggagca gggccagcac gacagcgcat gccgcgaccg
     7021 ttcgccccca ggccgctggt cgcgccaaaa cgagtctgct gagtagccaa cccacgcccc
     7081 aggcggccag cacgcccatg cacagccgcg ccagcaccac caaccgttcg ggtttggcga
     7141 tagcctccat accgggcagc cactccagca ggcggaaggg cagaggcaca ccagtctgca
     7201 tgcgccccac ctgcaactgc ggccccatcg acagcaccag cgcaaacagt gccagcccca
     7261 cccagaactt gcccctcgat gtgcgccaac ccacgatccc gcccaggact gagagcgtca
     7321 gggccgcgaa gccgagcgac aggtaatcgt gtatcggcgg ctcgttcacg ccggggcggt
     7381 cggcggggtc ccagccgagc agcgggtggt cgtggggcgg ggcgacgaag ctgagcaagt
     7441 cggcggagtg cgccaacctg tgctcgacgc ccgcctccac cgccagccgt gggttatcgc
     7501 ggtcacgcac caggcccaca agcaaaggca gcgtgagcac cagggccgca atgggcgcga
     7561 ccaccagggg agggaccagc ccgccccaac ctcgccccgc ctccttcccc ctcgcccctc
     7621 ggcgcgccca gagctggtac accacgtagg ccagcgagaa gatagccagg tacgccgcgt
     7681 agtacaggct gctcaaggcc gcgagcgcga agaacacccc cgccggcaag ccgtagcgca
     7741 gcctgcccgt gcgccaggcc atcacccacg cctcggcgta gaagggcatc cattgcagcg
     7801 acagcacttc aagctgcccc ttcagcgcgt cgagcgtgta gggcgtgaac gcgaacacca
     7861 caccccccac gaacgccaca aaggccaccg cccacggggg aagcggccaa ccctcgcccg
     7921 tggagacaaa ccgcaacgcc aggcgaaatg ccccgtaacc cgacagcgtg aaggccgcca
     7981 gcagcaccgt gttgtaggcg gcgggcagcc cgaacagcag ttgtggtagc agccctatca
     8041 accccagcgg caatcccagc gtgtggtagt agaggtccac gccgtagggg tagtagagca
     8101 tgccggtgtg gtaggggttg gtggcatgcg ctatcgcctc gcgcacccac cagaggttcc
     8161 acaggttctg gtcgcggtcg gccagcaagt caccgggcac gttggtgcca aactgcgtga
     8221 tggccggata ggtaaccagc acggcgagcg ccaggtagcc gagcagcgcc aggaggtgta
     8281 ccctcatgct tgctgtggcc tcacctgggg tgggtgtcac gccgccagcc gcgtcggggg
     8341 cttgcggtgc tgagtttgaa acactggaca tagcctgtgg agacatctta gcaaaagcgg
     8401 cgaccgggca caactgccct gcgatagccc cacatggacg ccctgtggct aaagccacgg
     8461 gctgagaagg ggcaaagtcc cctgcgggga ctaagcaaca tcaggatagt ctccgaagga
     8521 gacttcgtct ctggtaagcc cgtggcttta gccacagggc atccccactg ggcgaccctg
     8581 cagagaatga cgacacccgg acatctgccc tctaccagta cccttacccg caagggcagc
     8641 ggtaaagcaa aggagcagcc tttctggtac catgcggaag gcaaaggacg cgctaagcac
     8701 tattgcacag gaggctaccc aatgaccccc gcagataaag ccaggttcca caagaaactc
     8761 gacaccattg ttcaaggtgg cggtaccctc caggtggatg ggaaacgcac cgcccccggt
     8821 gatttgcttc cccacctgga ttgccttgaa ggccagtg
//
LOCUS       DASRWZ010000730         4855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_949720, whole genome shotgun sequence.
ACCESSION   DASRWZ010000730 DASRWZ010000000
VERSION     DASRWZ010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949720"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFH60_11575"
     CDS             complement(<1..618)
                     /locus_tag="VFH60_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6314466.1"
                     /translation="MGRMAGGGDPHEGQPIASAGEPLETARAAMIMVHGRGASAESIL
                     TLTEEFQRQGLAYVAPQAAGGTWYPYPFLAPMETNEPWLSSALLALEHTLALVNEHIP
                     AERVIWLGFSQGACLATEFVARHARLYGGVVGLSGGLIGPPGTPREYAGALDGTPVFL
                     GCSDRDAHIPAERVEESAEVFRRMGGTVTVRLYPGMGHTVNADEIV"
     gene            complement(618..1706)
                     /locus_tag="VFH60_11580"
     CDS             complement(618..1706)
                     /locus_tag="VFH60_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6314467.1"
                     /translation="MTVQGIHHITIVCADAQRTLDFYTQVLGHRLVKKTVNFDDPSSY
                     HLYFGNEAGAPGSIITFFEWPGAEPGHWGIGGTHHYAMQVADYDGLLKWKRRLNDLGL
                     PVDGPLDRHYFTSIYFTDPDGTILEIATVGPGWAIDEAPDRLGTEYREPPAEMLVRNR
                     DEERIAATTWPEPVPLITPDMALSAGLHHITAIGSDIERTHDFFGGLLGMRRVKMTSN
                     FDDPDSAHWYWGVGDGQPGTLITYFGRSAAKERRRKFGAGQTHHFALSVPDEDALLAW
                     RDRLVEANLSVTPVRDRVYFKSIYMTDPDGHIVELATAGPGFAVDEHQAELGSSLKLP
                     PWLEQDRERIEAKLKPLSAATALSVGQQ"
     gene            complement(1765..4671)
                     /locus_tag="VFH60_11585"
     CDS             complement(1765..4671)
                     /locus_tag="VFH60_11585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6314468.1"
                     /translation="MESTRKGNPKPTGGAYTALQSLLGAVAHTLGAGRVCLVSPSHEP
                     ECFTLLESGGEQSDRQRFQGGERDVSVPVMHGEQELARLVVVDAEPGLDRERLDAFAR
                     AAGYILGHSEQVSRAESMIEETRVLRELGMRLGQPTDLRELLDSMLAGVRRVLNADYA
                     SIAILEPDGRTRWLSMDGFRTSAYRDYLFPTGQGVSGRTIAAMRPVVVHGIGTDPDLP
                     AEEFPVHISEGGVSALGVPLIARGQPLGALILGSRTERQWHEQEIQLASVIANGAAVA
                     IDQNRAQKAESGQRQFLQAVIEHYPGVLVVVGPPPDWRIIIANSHFNKFLPEPYRSGK
                     SVVGLTAQELNTNDGARGEATRQMLAHVFETGEPLSFEQYESENPYTGTTYWNWQALP
                     VDNLAGDGERSLMLIAHDVTEVVRARQVVQEAAEVARARAEELEMVISHMADGVVIFD
                     RDGEVLRMNPAGEKLLGSSPLRAAPSGNQAAPYGLFAAEGETYETEELPVMRALRGEV
                     VVGENMLVRRPDQPEAVVRVSCAPLTGPQGDTVGAVAVLHDITQDKLVERLKDEFLSI
                     VSHELRTPLTAIIGYSDLMLRGVHGTLADRQAKVLNSVRANADRLLRLINDLLDVSKL
                     ESGAVHLRMDPVDVGEVAARTIAQTRILAVNAGVQISNLLPTRHLNKVQADDQKVQQV
                     LENLITNAIKHAGGGTITIDGYLTPLEPGDEALQVYEPPAQPLADGEAGSIVVSVRDT
                     GAGLESDQLARIWDKFYQVDTTVKRRSGGAGLGLTIVRNLVDLHGGRVWAYSQGPGKG
                     STFSFSLPVVQGEYGTFAQPGGTVPSRLLPSEPRRVRQPVMGTVLVAEDDADQREIIC
                     DMLELEGFEVVLAETGDEALDLAGQILPSAIALDVILPRRDGWEVLAALRQDPRTKDI
                     PVLIISVVDQTDFGRKLGADEYLLKPLDPRSLRTAIRRLVLAHERDASDKQQHN"
BASE COUNT          780 a         1646 c         1507 g          922 t
ORIGIN      
        1 gacgatctcg tcggcattta cggtgtgtcc catgccaggg tagagtcgaa ccgtcaccgt
       61 gccgcccata cgccggaaga cctcggccga ctcttcgacg cgctcggccg ggatgtgtgc
      121 gtcgcggtcg ctgcacccga ggaacacggg agtgccgtcg agagcgcctg catactcgcg
      181 tggcgtgccc ggcggaccta tcaacccacc actcaacccc acgaccccac cgtacagccg
      241 ggcgtgccgc gccacaaact cagtagccag gcaagccccc tgcgaaaagc ccagccagat
      301 gacgcgctcg gccgggatgt gctcgttgac gagcgccagg gtgtgctcaa gggccagcaa
      361 ggcagaggag agccacggct cgtttgtctc catcggcgcg aggaacgggt acgggtacca
      421 cgtaccccct gccgcctggg gtgcgacgta ggcgaggccc tgacgttgaa actcctctgt
      481 caaggtcaag atgctttccg cgcttgcccc acgcccgtgc accattatca ttgccgcccg
      541 cgccgtctca agcggttccc ccgcgctcgc gatgggctgc ccctcgtggg gatctccccc
      601 gcctgccatg cggcccatca ctgctgcccc actgaaagtg cggttgcggc gctgagcggc
      661 ttaagcttcg cctcgattcg ctcgcggtcc tgttcgagcc acggcgggag cttgaggctg
      721 cttcccaact ccgcctggtg ctcgtccacc gcgaagcccg gccctgcggt ggctagctcc
      781 acgatgtggc cgtcggggtc ggtcatgtag atgctcttga agtacacgcg gtcgcgcacg
      841 ggggtaacgc taaggttggc ctcgactaga cgatcgcgcc aggctaagag cgcgtcctcg
      901 tcaggtacgg agagcgcaaa gtggtgggtc tgaccggccc cgaatttgcg gcggcgttcc
      961 tttgcggcgc tgcggccgaa ataggtgatg agcgtccccg gttggccgtc ccccacgccc
     1021 cagtaccagt gtgccgagtc ggggtcatcg aagttggagg tcatcttgac gcgccgcatg
     1081 ccgagcaggc cgccgaagaa atcgtgtgtg cgctctatgt cgctgccgat ggccgtgatg
     1141 tggtgcagac cggcgctgag ggccatgtca ggcgtaatta agggcaccgg ctcaggccag
     1201 gtcgtggcgg cgatgcgctc ttcatcgcgg tttcgcacca gcatctcggc gggcggctcg
     1261 cggtactcgg tgcccagccg gtcgggggct tcgtcaatgg cccagcccgg ccccacggtg
     1321 gctatctcca ggatggtgcc gtccgggtcg gtgaaataga tggacgtgaa atagtgccgg
     1381 tccagcggcc catcgacggg caaccccagg tcgttgagcc tgcgcttcca cttcagcagg
     1441 ccgtcgtagt cggccacctg catggcgtag tggtgggtgc cgcctatgcc ccagtgcccc
     1501 ggctccgcgc ccggccactc gaagaaggtg attatcgagc cgggcgcgcc cgcttcattg
     1561 ccgaaataga ggtgataact ggaagggtca tcgaaattga ccgtcttttt gacgagccgg
     1621 tgccccagca cctgcgtgta gaagtcgagg gtgcgttgag cgtccgcgca tacgatagtt
     1681 atgtggtgaa taccctgtac cgtcatgtgg actcctcaat atacgtcgag caccgggttg
     1741 gcggccgctc attcgtgcct gacctcagtt gtgctgctgc ttgtctgacg cgtcacgctc
     1801 gtgggcaagc accaggcgtc gtatggcggt acgcaggctg cgggggtcta gcggcttgag
     1861 caggtactcg tccgctccca gcttcctccc gaagtccgtc tggtccacca ccgaaataat
     1921 cagcaccggg atgtctttgg tgcgcgggtc ttgcctcaag gcagcgagca cttcccagcc
     1981 gtcgcgcctc ggcagtatca cgtcaagggc tatggccgag ggcagaatct gccccgccag
     2041 gtccaacgcc tcgtcgcctg tctcggccag caccacctcg aagccttcca actccagcat
     2101 gtcgcagatg atttcgcgct ggtccgcgtc gtcctccgcc acgagcacag tacccatcac
     2161 gggctggcgg acgcgcctcg gctcagaagg caatagcctt gagggtactg tgccccccgg
     2221 ctgcgcgaac gtgccgtact ctccctgcac gaccggcagg ctgaagctga atgtgctgcc
     2281 tttgcctgga ccctggctgt aagcccacac ccgcccgccg tgcaggtcta caaggttacg
     2341 cactatagtc aagccaagcc cggcaccacc ggaccgccgc ttgacggtgg tatccacctg
     2401 gtagaactta tcccagatgc gcgctagctg gtccgactcg aggcctgccc cggtgtcccg
     2461 caccgacact acgatgctgc ctgcctctcc gtccgcgagc ggctgggcgg gaggctcgta
     2521 gacttgcagg gcctcatccc ccggttccag cggcgtcagg tagccatcta tcgtaatggt
     2581 gccgccgccc gcgtgtttta tcgcgttggt gatgaggttt tcgagtacct gctgcacttt
     2641 ctggtcgtcc gcctgcacct tgttcaaatg gcgggtcggg agcaggttcg atatttgcac
     2701 gcccgcgttc acggcgagga tgcgagtctg cgcgatggtg cgggcggcga cttctccgac
     2761 gtctaccggg tccatgcgca ggtgaaccgc ccccgactca agcttcgata cgtccaacag
     2821 gtcgtttata aggcggagca gccggtcggc gttggcgcgc accgagttta gcaccttggc
     2881 ctgccggtcg gccagggtac cgtgcacgcc cctcagcata aggtcgctgt aaccgatgat
     2941 ggcggttagc ggcgtcctca gctcgtggct cacgattgac aggaattcgt ccttgagacg
     3001 ttccacgagc ttgtcctggg tgatgtcgtg tagcacggcc accgcgccca cggtgtcgcc
     3061 ctgcgggccg gtgagagggg cacagctcac ccggacgacg gcctccggtt ggtccggcct
     3121 acgcaccagc atgttttcgc ccactaccac ttcaccgcgg agcgcgcgca tgacgggtag
     3181 ctcttccgtc tcatacgtct cgccctccgc cgcgaacagg ccgtagggag cggcctgatt
     3241 accggatggc gctgcgcgaa gcgggctgct gcccagcagc ttttcgcccg ccgggttcat
     3301 gcggagcact tcgccgtccc ggtcgaagat caccaccccg tccgccatgt ggctgatcac
     3361 catctccaac tcttcggccc gtgcccgcgc tacctcggcg gcctcctgca ccacctgccg
     3421 ggcgcgtacg acctcggtaa cgtcgtgggc gatgagcatg agggagcgct cgccgtcgcc
     3481 cgccaggttg tccaccggca gggcctgcca gttccagtac gtggtcccgg tgtatggatt
     3541 ttccgactcg tactgctcga aagagagggg ttcgccggtc tcgaagacgt gcgcgagcat
     3601 ctgccgcgta gcctcaccgc gcgcgccatc gttggtgttc agttcctgcg ccgtaaggcc
     3661 aactacggac ttgccgctgc gatacggctc cgggaggaac ttgttgaagt ggctattggc
     3721 aatgataatt cgccagtcgg gaggcggccc cacgaccacg agcacgccag ggtagtgctc
     3781 gattaccgct tgcaggaact gtcgctgccc gctctcggcc ttctgtgccc ggttctggtc
     3841 gattgcgacg gccgccccgt tcgcgatgac ggaagcaagc tgtatctcct gctcgtgcca
     3901 ctgccgttcc gtgcggctgc cgaggatgag tgcgccaagc ggctgccccc tggctataag
     3961 aggtacgccg agcgccgaga cgccaccctc ggaaatgtgc accgggaact cctcggccgg
     4021 caggtccggg tcggtgccaa tgccatgtac caccaccggg cgcatggcgg caatcgttct
     4081 tcccgatacc ccttgtcccg tggggaataa gtagtcgcgg taggccgagg tgcggaagcc
     4141 gtccattgac agccagcgcg tcctgccgtc cggctccaat atggcaatgc tggcgtagtc
     4201 tgcattgagc acccggcgca cgcccgcgag cattgagtcc aacagctcgc gcaggtcggt
     4261 gggctggccg aggcgcatgc caagctcgcg caacacccgt gtctcttcaa tcatcgactc
     4321 ggcgcgcgac acctgctcgg aatgccccag gatatagccc gccgccctgg cgaaggcatc
     4381 cagacgctcc cggtccagcc ccggctcggc atcgaccact accaaccggg caagctcctg
     4441 ctcgccatgc atgacaggca ccgagacgtc gcgctcgcca ccctggaacc tctgccggtc
     4501 tgactgttcg ccgcccgact ccaggagcgt gaaacactca ggctcgtggc tcggtgagac
     4561 aaggcagacc ctgcccgctc ccaacgtgtg ggccaccgct cccagcagac tctgcaacgc
     4621 ggtgtaagcc ccacccgtgg gcttcgggtt cccctttctt gtggactcca tcccccctgt
     4681 cctctcttac ggcttccatg cacccttgcg gtcccgctgc cggctatatg cggtcgcaag
     4741 gcgcgaccca ctcgcgtcta catgcgcatt atcacccgcc tgtacgcttc ggcaaaagcg
     4801 acaccgtacg gctcgcctat ctgggctgca cgcatcttta tgcgattgcc cagtt
//
LOCUS       DASRWZ010000731         4329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1155452, whole genome shotgun sequence.
ACCESSION   DASRWZ010000731 DASRWZ010000000
VERSION     DASRWZ010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155452"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VFH60_11590"
     CDS             complement(<1..1043)
                     /locus_tag="VFH60_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314469.1"
                     /translation="MLLLLLLFAGVLSGCEVPKPPPELPTATPGSSNPESPNFEMGKT
                     PVRIEQNKGYTPVPGAPGNIFFVRATGLWRISPDGSGETRLASLGVTNPPQPSPDGSL
                     VAFTTADAVYVIPSSGGEPRKLTGGSIADRQRLGWTPDGNMVGYFTFDATIMGREIAW
                     AVPVEGGDPVEILPLTGSAVSRGPLYEKSVRWSPDGLFVAVSGVDNPFWLLRWPLSTG
                     REGDMRQVPGGEPEWSPDGRTIIYAETLSGALLVYGVLQAEATPFRNELRYIGTGLGE
                     YAQGPGPRFSPASTGSDSDLIAFRSRTQQGEPQVALRRRSVGELSPLPPLTNNPAWAP
                     GGDRLVVETGYLK"
     gene            1553..2314
                     /gene="recO"
                     /locus_tag="VFH60_11595"
     CDS             1553..2314
                     /gene="recO"
                     /locus_tag="VFH60_11595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET6314470.1"
                     /translation="MSAREHLYKSEAVVIKRSDLGEADKILTIFTPHFGKLRVVAKGV
                     RKVSSRLAGHVELFTRSQMLLAKARNLDIITQSETLDSYRPLHDDFDRVANAFYAAEL
                     LDALTPDELESYPIYKLMVESFGLLSTDPDPARVLRWLELQLLGYLGYAPELTKCVEC
                     RNDIAPTVNTFSAALGGVLCASCRRPGVGRDLSVNGLKLLRLMQRNPYSAVSRVRLDP
                     DLHHELQSVLQSYLTYLLERELRSTHFIKGLRNEG"
     gene            2363..2695
                     /locus_tag="VFH60_11600"
     CDS             2363..2695
                     /locus_tag="VFH60_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314471.1"
                     /translation="MDRSTHKSVWYRWSPKRLWTPFAPQDVGKLLITRRYLEYSSRRL
                     SVRIRIADISSISMGRQGSDWINKWVKVEYGRGKVAYFADGRFLGWRGILGGTRRLLD
                     RLRLARRS"
     gene            2697..2984
                     /locus_tag="VFH60_11605"
     CDS             2697..2984
                     /locus_tag="VFH60_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HET6314472.1"
                     /translation="MSAYVIVEIDVTDPEGYEDYKGPAAATVEASGGRYLVRGGKTEV
                     LEGDWQPKRLVVLEFDSVEQAKVWWHSEEYAPLKDIRHRTTNSRMIVVEGI"
     gene            complement(3358..4140)
                     /locus_tag="VFH60_11610"
     CDS             complement(3358..4140)
                     /locus_tag="VFH60_11610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6314473.1"
                     /translation="MELEGKVALVTGGGRGLGRATALALAGLGARVVALARSLPEVEE
                     TARLVRDEYGFGRSMAIRADVTRESEVVEAFEQVRRRWGGVDILVNNAGETGATKPIG
                     QLSLAEWRQAMDVNMTGVFLCSREALPYMQSRRWGRIVNVSSASASVAVPSMAPYSVA
                     KAAVEHFTRILAAEGAPHGIVAVALRPGIVDTRMQEDMRSRPTEAISPELRALYVAYD
                     RRGMLAPPERPARFIAYLCTNRAGDMNGRVLDADEMDALLTR"
BASE COUNT          875 a         1385 c         1313 g          756 t
ORIGIN      
        1 tccttgagat atccggtctc gaccaccagg cggtcaccgc cgggagccca ggcggggttg
       61 ttggtgagtg gaggcagcgg tgatagctcg cccacgctgc gccgcctgag ggccacttga
      121 ggctcgccct gctgggtgcg gctgcggaag gctatcaggt cgctgtcgga gccggtcgat
      181 gccggggaga agcgcggccc cggaccctga gcgtactcgc ccagccccgt gccgatgtag
      241 cgcaattcgt tgcgaaacgg cgtggcctcc gcctgcaaca ccccgtagac cagcagcgcg
      301 cccgaaagcg tctcggcgta aatgatggtg cggccatcgg gcgaccactc aggctcgccc
      361 cccggcacct ggcgcatgtc gccctcgcgg cccgtagaga gcggccagcg tagcagccag
      421 aagggattgt caacccccga caccgccacg aacagcccat ccggcgacca gcgtaccgat
      481 ttttcgtaca gaggcccccg cgacacggcg gacccggtga gcggcagaat ctcaacagga
      541 tcgccacctt ccaccggcac ggcccaggct atctccctgc ccataatagt ggcatcgaag
      601 gtaaagtagc ccaccatatt gccatcaggg gtccagccca ggcgctggcg gtcggctatg
      661 ctccctccgg tcagcttgcg gggttcgccc ccagaggagg ggataacata cacggcgtcg
      721 gccgtagtga aggccaccag gctgccatcg ggcgagggtt ggggcgggtt ggtgacgccc
      781 agggacgcca accgggtctc gccggagcca tcgggggata tgcgccatag gccggtcgct
      841 ctcacgaaga agatgttgcc cggcgctcca ggcaccggcg tgtagccctt gttctgctcg
      901 atgcgaacgg gggtcttgcc catctcgaag ttggggcttt cggggttgga ggagccaggg
      961 gtcgcggtcg gcagctcggg cgggggcttg ggcacttcgc accccgataa cacccccgcg
     1021 aacagcagca gtagcaatag cacacacgat aaccggcgca tgtttcagta tagcacagca
     1081 cgccgcacgt accaggcaga gagaaagcgc gcgccccaat tttacggtat tgttaatgct
     1141 cggcgataag gcccccgcgt gagaccatac ccgatgttca gtgcaaagcc tcgcccccgc
     1201 catcctgagc ggagcgaagg atcagccgcc cgttcttgat agccacttct acagctccca
     1261 aagccccctc tgtcattctg aacgtagtga agaatcttgg atgctgcctt tgcttctacg
     1321 ttgaacgtga gcactatgat acgtaaggtt caacagcatc cgtataccag cttctttaga
     1381 ttcttcacta cgttcagaat gacatgggcg ggactctatc cctcctcacc aacagtgccg
     1441 acagcgagac agattgccgc cgctacacac gaaacgcgcg taatcgggta taattaggag
     1501 gtacgtagca caccggcgca gaggcaaaca gagctagaga agcgagttag agatgagcgc
     1561 gagagagcat ttatacaagt cggaagcggt ggtcatcaag cggtcggacc tcggtgaggc
     1621 cgacaagata ctgaccatct tcacccccca cttcggcaag ctgcgcgtgg tcgccaaggg
     1681 cgtgcgcaag gtaagcagcc gcctggccgg gcacgtcgag ctattcacca ggagccagat
     1741 gctcctcgcc aaggcccgca acctcgacat catcactcag agcgagacgc tcgatagcta
     1801 tcgccccctg cacgacgact tcgaccgcgt agccaatgcc ttctacgccg ccgagctgct
     1861 cgacgccctc acccccgacg agcttgagag ctaccccatc tacaagctca tggtggaaag
     1921 cttcggcctc ctctccaccg atcccgaccc cgcccgcgtg ctgcggtggc tggaattgca
     1981 gctcctcggc tacctcggct acgcccccga gcttaccaag tgcgtggagt gccgcaacga
     2041 catcgcgccc acggtaaaca ccttcagcgc cgcgctgggc ggggtgctgt gcgcctcttg
     2101 ccgcaggccg ggcgtgggcc gcgacctctc ggtaaacggc ctcaagctgc tcaggctgat
     2161 gcagcgcaac ccctacagcg ccgtgagccg cgtccgcctc gaccccgacc tgcaccacga
     2221 gctgcaatcg gtcctccaga gctacctcac ctacctcctc gaacgcgaac tccgctccac
     2281 ccacttcatc aaggggctta ggaacgaagg gtaagcataa agcgggaccg cgtgcttcga
     2341 tgccgggcag aggagaatgg cgatggatag gagtacgcac aagagcgttt ggtacaggtg
     2401 gtcgcccaaa aggctctgga caccgttcgc cccgcaggac gtgggcaagc ttcttatcac
     2461 ccggcgttac ctcgaataca gcagcagaag gctcagcgtg cgtattcgca tcgcggacat
     2521 cagcagcatc tcgatgggca ggcaaggcag cgactggata aacaagtggg tcaaagttga
     2581 gtatggccgc ggtaaggtgg cctactttgc tgatgggcga ttcctgggct ggaggggaat
     2641 cctgggcggt acgaggagac tacttgatag gctcaggcta gcaaggagga gttgagatgt
     2701 ctgcatacgt gattgtcgaa attgacgtga cggacccgga ggggtacgag gactacaaag
     2761 gccctgcggc agccacggta gaggcgtcgg gtggccgcta cctggtgcgt gggggcaaga
     2821 cggaggtgct tgagggcgat tggcagccca agcgcctggt agtgctagag ttcgatagcg
     2881 tggagcaagc aaaggtgtgg tggcactcgg aggagtacgc tcccctcaag gatataagac
     2941 accgcacgac caacagcagg atgattgtgg tcgaggggat ttagtccgca ttgacgaacc
     3001 agcggaaccc tattccccct tgtcatcctg agagcagcga aggatcagtg gcccgtgttt
     3061 gactctccct actattgctc ccatgttcat cttaccgcca gcatcctgag cgttgaagag
     3121 tctcgcctac ccacctgatc cttcgctacg ctcaggatga caagggggaa taaagtccta
     3181 cccatccagc attgggacta gcggcagggc acaacgcatc gtgcccccat gcaccctaaa
     3241 tgctcgtaat ctccgcatat catttatatc ctttgctcac tttgcgctct ttgcggtttc
     3301 acgtctcacg tgtactatcg cataggagat gcaatcgtcg gaacgcccta tgtctattta
     3361 ccgagtcagg agcgcgtcca tctcgtcggc gtccaggacg cggccgttca tgtcgcctgc
     3421 gcggttggtg cacaggtagg cgatgaagcg ggcggggcgt tcgggtgggg cgagcatgcc
     3481 acggcggtcg taggcgacgt acagggcgcg cagttcgggc gaaatagcct cggtggggcg
     3541 ggaacgcatg tcttcctgca tgcgggtgtc tactatgccg ggccgcaggg ccaccgctac
     3601 gatgccgtgg ggcgcgccct cagcggccag gatgcgggtg aagtgctcca cggcggcctt
     3661 ggctacgctg taaggggcca tcgagggcac cgccaccgat gcggaggccg acgacacgtt
     3721 gacgatgcgg ccccaacgtc tgctttgcat gtatggcagg gcctcgcgag agcagaggaa
     3781 cacccccgtc atgttcacgt ccatcgcctg ccgccactcc gcgagggaaa gttggcctat
     3841 gggcttggtc gcccccgtct cgcccgcgtt gttcaccagg atatcgacgc cgccccagcg
     3901 cctgcgcacc tgctcgaacg cctctactac ctccgactcg cgcgtcacgt cggcgcggat
     3961 agccatcgag cgcccgaagc catactcgtc gcgcaccagg cgggccgtct cctcaacctc
     4021 cggcagcgac cgcgccagcg cgacgaccct cgcccccaac ccggcaagcg cgagcgccgt
     4081 cgccctgccc agccctcgcc cgccgccagt caccaatgct accttgccct caagctccat
     4141 tgcctgaatt cctcccttgc gtgccaggag cgcccacccc atgcacaccc caatcttagc
     4201 atgctccccc acgcctgtcc agcgtcattg aattagagac ggaaacgcaa agacgcaaag
     4261 gacgcaaagg acgcaaagga cgcaaaggac gcaaaggacg cgcagagatt acgataatgg
     4321 tgttctaag
//
LOCUS       DASRWZ010000732         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_744270, whole genome shotgun sequence.
ACCESSION   DASRWZ010000732 DASRWZ010000000
VERSION     DASRWZ010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744270"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VFH60_11615"
     CDS             <1..839
                     /locus_tag="VFH60_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314474.1"
                     /translation="MARFVKSYETERWGKRHLLTVSVFGAFPEPGYIDLILRHPELDF
                     ATTHVYQNDAKDNPTDTISGAITMRDAVRFAYAHIVDMRPYMDTESGPIFGYMHTRKR
                     PTAEFENEYYHNMTWAHLSTGGAGSGMRWPFRDPHTLSPGMHDVMLGMSRFVKANPLR
                     WLDFSPRPLDDSLRVVPRDAAGGDTQTQVLPFGCSDGRQALVWLLRDTRVAEGQAASG
                     PIDLLLPGMIAGGYIIEFWETYEGNKVGKAQVAVGAGDANIGLALPDFGRDMAIAVRL
                     GE"
     gene            1082..1981
                     /locus_tag="VFH60_11620"
     CDS             1082..1981
                     /locus_tag="VFH60_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HET6314475.1"
                     /translation="MIEAIKFWNEPNNLSHWDFEIDPGWVEFAQMTGLAARTVRDLCP
                     DLCLVLGGISPIDPEFILTLQKQGVLEVLDAVAVHGFPRDWNHWQINDWPAKVAEIEA
                     VSHLPVWVTEVGVSTFGADEVQVFGLQRTADLLLGRVPRVFWYSLFDLPPAWEATTRH
                     KESEGSSYYRHFYMGLIRADGTPKPAIDHFNPEMGICQWFHFEDHRLELAVEWLRRMG
                     VKRLRTGISWADWHRPNAVEWFDRQMRAIEEFDTTVTLCFTPPSIGKRPHHTSPPVDL
                     GEFAYFASQVVERYALNHLAVPR"
     gene            1988..2605
                     /locus_tag="VFH60_11625"
     CDS             1988..2605
                     /locus_tag="VFH60_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6314476.1"
                     /translation="MTTFLLIRHASHALLKRTLVGRTPGVPLDEEGRAQAERLAERLA
                     DLKPLALYSSPVQRAMETAAPLSERWGLPVQMSKEFIEIDFGEWAGRDFSDLRPLQQW
                     DYFNSYRSGTRPSGGELMLEVQARAVTGLQRLHAEHPGSLIAIVSHGDVIRAALNYYL
                     GAALDHFRRLEVSPASVSLLELQDWGPRVLLVNHLGDLPQVSADM"
BASE COUNT          605 a         1054 c          966 g          599 t
ORIGIN      
        1 agatggcgcg gttcgtcaag agctatgaga ccgagaggtg gggcaagcgg cacctgctca
       61 ccgtgtcggt gttcggcgcg ttccccgaac cgggctacat cgacctcata ctgcggcacc
      121 ctgagcttga cttcgccacc acccacgtct accagaacga cgccaaagac aaccccaccg
      181 atacgatcag tggggctatc accatgcgcg acgccgtgcg cttcgcctac gcccatatag
      241 ttgacatgcg cccttacatg gacaccgaaa gcgggcctat cttcggctac atgcacaccc
      301 gcaagcgccc gaccgccgag ttcgagaacg agtactacca caacatgacc tgggcgcacc
      361 tctccacagg cggggcgggg agcggcatgc gctggccttt ccgcgacccc cacaccctct
      421 cccccggcat gcacgacgtg atgctgggca tgtcccgctt cgtcaaggcc aaccccctcc
      481 gctggctcga cttctccccg cgcccactgg acgactccct acgtgtagta ccccgcgacg
      541 ccgctggggg agacacgcaa acgcaagtgc tgccgtttgg ctgctccgac gggcggcagg
      601 cgctcgtctg gctgctacgt gacacgcgtg tcgctgaagg ccaggcagca agtgggccta
      661 tcgacctgtt gctccccggc atgatagcgg gcgggtatat aatagagttt tgggagacgt
      721 acgaggggaa caaagttggg aaggcacagg tggctgtcgg cgcgggtgat gctaatatcg
      781 ggttggcact gccggacttt gggcgggaca tggcgattgc cgtgcggctg ggggagtaat
      841 gctgtggttt cggcggcata gtcctggggg gacgccctat gtggacgccc tgtggctaaa
      901 gccacgggct gacaaggggc gaagtcccct acggggactg aggaacgtca tgtagtctcc
      961 gaagcagact tcgtctctgg taagcccgcc aattcattgg cagggcgtcg tgcaggtcaa
     1021 ccatgctgga caacactgac ccttcccacc cgaccgtcgc ccgcgtgcca aaggagacac
     1081 tatgatcgaa gccatcaagt tctggaacga gcctaacaac ctgtcgcact gggacttcga
     1141 gatcgacccc ggctgggtgg agttcgcgca gatgaccggc ctggccgctc gcacggtgcg
     1201 cgacctctgc cccgacctgt gcctggtgct gggcggcata tcccctatag acccggagtt
     1261 catcctcacc ctacagaagc agggcgtgct ggaggtgctc gacgcggtcg cggtgcatgg
     1321 cttcccgcgc gactggaacc actggcagat caacgactgg cccgccaagg tggccgagat
     1381 agaggccgtg agccacctgc ccgtctgggt gaccgaggta ggagtgtcca cgttcggcgc
     1441 ggacgaggtg caggtgttcg gcctgcaacg caccgccgat ctgctcctgg gccgggtgcc
     1501 ccgcgtcttc tggtacagcc tgttcgacct accgcccgcc tgggaggcca caactcgaca
     1561 taaagagagc gagggcagct cctattaccg ccacttctac atgggcctca ttcgcgccga
     1621 cggcaccccc aagcccgcta tcgaccattt caatccggag atgggcatct gccagtggtt
     1681 tcatttcgag gaccacaggc tcgagctagc cgtggagtgg ctcaggcgca tgggcgtgaa
     1741 gaggctgcgc accggcatta gctgggccga ctggcaccgt cccaacgccg tggagtggtt
     1801 cgaccggcag atgcgcgcga tcgaggagtt cgacaccacg gtgacgctct gcttcacgcc
     1861 cccctcaatc ggcaagcgcc cgcaccacac tagcccacct gtggacctgg gcgagttcgc
     1921 ctatttcgcc tcgcaggtgg tagagcggta cgccctcaac cacctggcgg tgccccgcta
     1981 ggcaattatg accactttcc tgctcatccg ccacgcttcc cacgccctgc taaagcgcac
     2041 cctggttgga cgcacccccg gcgtgccact ggacgaggag ggcagggcgc aggccgaacg
     2101 tctggcggaa cgactggctg acctgaagcc gctggccctg tacagcagcc ccgtgcagcg
     2161 cgcgatggaa acggcggccc ccctctccga gcgctggggc ctgccggtgc agatgagcaa
     2221 ggagttcatc gagatcgact ttggagagtg ggccggccgt gacttcagcg acctgcgccc
     2281 gctccaacag tgggactatt tcaacagcta tcgtagcggc acccgcccct cgggaggcga
     2341 gttgatgctt gaggtacagg cccgcgcggt gacgggcctc caacgcctcc acgccgagca
     2401 ccccggctcc cttattgcca tagtgtccca cggcgacgta attcgcgcgg ccctcaacta
     2461 ctacctgggt gccgcgctag accacttccg acgcctcgaa gtcagccccg cctcggttag
     2521 cctcctcgaa ctgcaagact ggggtccgcg cgttctgctg gtaaaccacc tgggcgacct
     2581 gccccaagtt tcggcagaca tgtaataagt gggagggtcc ttaacggcaa gacgccacgg
     2641 cactaaggac cacgccagga gtgtttcggt ctagaaagtg caaagcggac cagggtacgt
     2701 gctgtcgggc tataatctga ctgcctcggc gctgtgccct ggattttcgc cgtcacgcta
     2761 attactcttt ccaggactgt cccttgatat ttgtgacgct ttttgtgacg gtcagtgcgg
     2821 caaatttata agtatactat ggctacttgt agttcctagg ggtgcctatg gtgtcctgcg
     2881 atgtacggaa gcaaagccgg tatctgcaac ctcacggttc ttcctatgca ggcccggccg
     2941 cttatgcatg tccacgcgtt acaagcatct ccgcgtttcc ggccactccc aggcggcaat
     3001 agcacccaat ctcatcaatc ggttctcagc accccgaccg gttagcccca ttactcaccg
     3061 aagaggagca acaaatgcaa gctaccgcga ctccaaccgt agtacctaca tcaactcccg
     3121 tcccgccgtt cgatccatca tcggtaatct cgtcattagg caccgccgac ttttggaccg
     3181 cgctctgggt cacgcttctc gcaggcgcgg tcgggggggt ggga
//
LOCUS       DASRWZ010000733         4276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_402462, whole genome shotgun sequence.
ACCESSION   DASRWZ010000733 DASRWZ010000000
VERSION     DASRWZ010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402462"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFH60_11630"
     CDS             complement(<1..328)
                     /locus_tag="VFH60_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314477.1"
                     /translation="MKRSIFALIALFVSAAIQTSCDFGGNAPLPGDATMTPTNPSGSV
                     PTEPPTPGSYPTFGQAPDLSWISGRVTFTKIQGSCVYIFAKEVPAPAAAEPTSTGPVV
                     GTSVAND"
     gene            complement(859..2265)
                     /locus_tag="VFH60_11635"
     CDS             complement(859..2265)
                     /locus_tag="VFH60_11635"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET6314478.1"
                     /translation="MVMFDALRETFPAEIVRRLLKSIVHARLMPLAEDGDLEALVAEL
                     HLVAEEAYEGVLGTQLKDMDEKLKDFFHIGDVYYEVAGEGEVDYNKLSRTLGLTPNLI
                     QHMRDEEMGYEDALDFLGEIIREGYEKQQQSRLDQLKKYISDESAKISSRDREAGVSA
                     VDLRVRGLLETIDSGFNLSSASAARYVDAEKGQQGGRSSMDMGGSDSVVDEVQEQLEA
                     LYAEVEQTLGDVFMETNQQMPLIAMVDNRLPRSVYRQIEERLGLEGLEQVENIPLSQL
                     PQEVADIVNEAFVRWQEADLMLRVIDYLWTRHLTTMEGLRHSIGLQAYGQKDPLIQYK
                     IKAYDLFEDLKGEIRQLVVLNVLLMGEKAEAARAQNRQAAQTQAKAAQQQSAAQPQPS
                     RAAVGAGVGAGNGRAAHGGNGKQQGKGQQASRPSNQASRPAQGPPVGAGAAMKGKLGR
                     NDPCFCGSGRKYKHCHGR"
     gene            complement(2281..4276)
                     /gene="secA"
                     /locus_tag="VFH60_11640"
                     /pseudo
     CDS             complement(2281..4276)
                     /gene="secA"
                     /locus_tag="VFH60_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
BASE COUNT          662 a         1376 c         1344 g          894 t
ORIGIN      
        1 tgtcattcgc cacggacgta ccaaccacgg ggccggtgga ggtcggttcg gcggccgcgg
       61 gtgccggcac ttccttcgcg aagatgtaga cacagctacc ctgtatcttt gtgaaggtca
      121 cccgccccga aatccagcta aggtcggggg cctggccgaa ggttgggtat gaacctgggg
      181 tgggcggctc ggtgggtacg gagccggaag ggttggtggg ggtcatggtg gcgtctcctg
      241 gaaggggcgc gttgccgccg aagtcgcacg aggtctgtat cgcggctgac acgaacaatg
      301 caatcaaggc aaagatgctg cgcttcaagt cgccatcctt tctgtagtgc tgggccgcct
      361 tgcatggtcg ccctgtgggg gacgccctgc caatgaatta gcgggctgac aagagacgaa
      421 gtctccttcg gagactacat gagcaacatc cgcaccgcac attccgaatc cgagcaaggc
      481 cccgcactac ttataaggtg caatacaagt aacaggatcg ccagccgacg ccgggcgatt
      541 gatatcgggg gctacacgtt gcaaagtctg cccagcggca gactggccgc ctgcgcagga
      601 gttgctttca agagacaatg tggccgaagg agtccgccgc agggcggact ttgtatgtgt
      661 agcccgcgat ttcaatcgcc gggcgtcggc ctttggctgc tgtatccaat gacctgtggg
      721 acaccaactg gtgacttgtg ctgctcctat tggcatcatc gggcgacggc cttcgcatta
      781 cccgtcccaa aaccactgag gaccaagccc aaacgctcag tcctcagtcc tcagtcctca
      841 gtcctcagtc ctcagtactt atctgccgtg acagtgcttg tatttgcgac cgctgccgca
      901 gaagcagggg tcgttgcggc ctagcttgcc cttcatcgcg gcgcccgcgc ccacgggggg
      961 tccctgtgcc ggacgcgagg cctggttgct ggggcgtgag gcttgctggc ccttgccctg
     1021 ctgcttgccg ttgccaccgt gggccgccct gccgtttccg gcacccacgc ccgcacccac
     1081 tgctgcccgc gagggctggg gttgggccgc agattgctgc tgggctgcct tggcctgtgt
     1141 ctgggcggcc tgccggttct gggcgcgtgc cgcctcggcc ttctcgccca tcagcagcac
     1201 gttcagcact actagctgcc ggatttcccc cttgaggtcc tcgaacaggt cgtaggcttt
     1261 gatcttgtac tggatcaggg ggtctttctg gccgtaagcc tgcaagccga tagagtggcg
     1321 caggccctcc atcgtggtca ggtgccgcgt ccacaggtag tcgatcaccc gcagcatcag
     1381 gtcggcctcc tgccagcgca cgaaagcctc gttgactatg tccgcgacct cctggggaag
     1441 ctggctgagg gggatgttct ccacctgctc caacccctca agtcccagcc gctcctctat
     1501 ctggcggtag accgagcggg gcaggcggtt gtccaccatc gcgatgaggg gcatctgctg
     1561 gttggtctcc atgaagacgt cgcccagcgt ctgctcgacc tcggcataga gcgcctcaag
     1621 ctgctcctgc acctcgtcca ccacgctgtc ggagccaccc atgtccatgc tcgacctgcc
     1681 gccctgctgg cccttctcgg cgtccacgta gcgcgccgcc gaggccgacg ataggttgaa
     1741 gccggagtct atcgtctcaa gcaggccgcg cacgcgcagg tctaccgcgc tgacgcccgc
     1801 ctcgcggtcc ctgctgctta tcttcgccga ctcgtcggat atatacttct tcaactggtc
     1861 gaggcgcgat tgctgctgct tttcgtaacc ctcgcgtatg atctcgccca ggaagtcgag
     1921 cgcgtcctcg tagcccatct cctcgtcccg catgtgctgg atgaggttgg gagtgaggcc
     1981 gagcgtgcgg ctcagcttgt tgtagtctac ctcgccctcg cccgccactt cgtagtacac
     2041 gtcaccgata tggaagaagt ccttcagctt ctcgtccatg tccttgagct gcgtgccgag
     2101 cacaccctcg taagcctcct cggccaccag gtgcagctcc gcgaccaaag cttccaggtc
     2161 gccatcttcg gccaggggca tcaggcgggc gtgcacgatg gacttgagca ggcggcgcac
     2221 tatctcggcg gggaaggtct cgcgcagggc gtcgaacatg accatatagc cgccggggac
     2281 ggccccccgc ttgcctttgc cctcgccgcc caccgcctga gcgcctatct cctgcacctt
     2341 cgcggcgatc atctcctcga tgatgaggcg cgggtcgccc tgcctgagga tgtagcggcg
     2401 gtcgctgtag acgtagtcgc gctgcgagtt caccacgtcg tcgtactcta cgaggtgctt
     2461 acgggtgtcg aagttgtagc cctccatctt cacctgggcc tgctccaccg agcgggagat
     2521 catcgagtgc tcgatcggca ggtcttcttc ccacaggctc ttgaagatgt tggagttgac
     2581 gatgttctgg ttcacgcccg aacggcgcat gatttcgtct tcgatagaga cgtagaagcg
     2641 cgacgagccg ttgtcgccct ggcgtccggc gcggcctcgt agctggttgt cgatgcggcg
     2701 gctctcatgc cgctccgtgc cgatgatgtg taggccccca agctcgttca cgccgaagcc
     2761 cagcacgatg tcggtcccgc ggcctgccat gttggtggcg atggtgatgg ccccgcgctg
     2821 tccggcctgg gccacgatgc tggcctcgcg ctcgtgctgc ttcgcgttga gcagagagtg
     2881 gggtaggccc gcttcttcca gcatcttgct gagttcttcc gacttctcga ttgagacggt
     2941 acccacgagc accgggcggc cctgctcgtg aagctccata atctcctcca ccacggcccg
     3001 gaacttggcg gcctcggtgc ggtagatgcg gtcggggttg tccacgcgaa tcatagcgcg
     3061 gtgcgtgggg atgggggtca cttcgagctt gtaaatcttg aagaactcct cggcctcggt
     3121 ggccgccgta ccggtcatac cggccagctt ggggtagagg cggaagtagt tctggatggt
     3181 aatcgtggcg aaggtctggc tctccgagcg cacgctgatg ccttccttgg cctcgatagc
     3241 ctcgtgcagg ccctccgagt agcggcggcc ttgcatcagg cggcccgtga actcgtccac
     3301 gattactact tctaccggcg ctttggggtt gagcttctcg tgcgcgctca ccaccttgcc
     3361 ttcctgccac agcacgtagt ccttgtcgcg cctgaacagc acgtgggcct tcagcgcgtt
     3421 gtccacgccg tacacaagct ggaagttgtc ggggtggtac aggttgtcca ccttgaagaa
     3481 ctgctcggcg cggtcgatgc ctgcatcggt gagggccacg ctacgggtct tctcgtcctg
     3541 ggtgtagtcg atctcggccc gcaactgctt ggcgaaagcg gcggcgcgca cgtgcacctc
     3601 ggcgttgccc tcgcccgcgc ccgaaatgat gagcggggtg cgagcctcgt cgatgaggat
     3661 gttgtccacc tcgtccacga tgcagtagtg gtgcgggcgc tgcactatat cgtcggggtt
     3721 gttggagatg ttgtcgcgca aatagtcgaa gccgaactcc gagttcgagc cgtacgtgat
     3781 gtccgactgg tactcggcct tgcgctccca cggggccatg ctgctcagga tcgcccccac
     3841 cgacaggccc agcatcttgt agacaggccc catccagtcg gagtcgcggc ggataaggta
     3901 ctcgttcacg gtgcacaggt gcgcgccgcg cgcggtcagg ccgttgaggg cgaggggggc
     3961 cacggccacc agcgtcttgc cttcacccgt cttcatctcg gatatcttgc cctcgtggag
     4021 cacgataccg cccatgagct gcacgtcgta gggccgcatg ccgttggtgc ggcgcgaggc
     4081 ctcccacacg caggcaaaca cctcagggat gaggtcgctg agcacccgct gctcgtcctt
     4141 gcgctgctcc ttgcggaact cgtcgctcag ggccttcagg ctgtcgtgag cgccccggtt
     4201 ggtgggccgt agctcgttca tcagctcggt gaaggtggct tcctccaggc tgtcgcgccg
     4261 ccgcatgaac tcgccc
//
LOCUS       DASRWZ010000734         3345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_58622,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000734 DASRWZ010000000
VERSION     DASRWZ010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58622"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..>3345
                     /locus_tag="VFH60_11645"
     CDS             24..>3345
                     /locus_tag="VFH60_11645"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HET6314479.1"
                     /translation="MTTQVLGLVALPIAAVLMRGVPDRGYTAAKALGLLLVTWPAFTI
                     AMTGVVPYGRVLLFGCLLLVAALSTWLLLRDNRALLWELRAHFRTRSLVRHLVAAEVL
                     FAVLFAFWAWMRAFNPEILDQEKFMDFGILNSILRTGSLPPPDMWFAGQSLNYYYFGY
                     IIIAAMSSLSGVAASVSFNIANAFLFAMTGLGAYGVVYNLIVGTILRRAGRPTLTQAP
                     PPPPPAPSTARGRRGNKPAPAPARPLPTRQVQAQQLAFAGATSGTYSTVVDEEVAAAP
                     LPARRNRAAAQRDEARYAEPVEAGEGTDDPTRRVPFYLSPILYGVLAALMIVAMGNLA
                     APFGTKDDTTEEATINGNGWTFCFFCAKKDYDYWWDPSRIILDYRTVQQPDGSMLKSK
                     VGYETINEFPAFSFVLADMHPHVMGLPFVLLAVMVAYALGRRRVAGSNLWRDGLPRSL
                     GGWLGLLLSSIIVGALYAINTWDYPTYLLVMLGGLALPYIAAGHREEGGRRWARPFVV
                     QAFLLIAISLLAYLPFHLTFKSFAGGGPVALPENIANIPVLGGLLRSLSGLFLVNTAD
                     KTITGFMVIFGIFLLSLTVWLIYEFATYMRKRARASEEGVNSLLVFGITLMVILLVAL
                     WTRFPLLALLLPLIIMAGYLIWQEPRRTERNMVLGVFLVAATIGLGIEIIYLHDNFGN
                     RMNTLFKFYYQMWVLWALAAAYGFWRTLQAAFGANRLMSAGRGYDRVECAVNDTPNGL
                     RVLAGTWAAVFLLLVLSALPYAWFGSQQRNMINKTAPIGLDGMETLRMRNPSDYEAVN
                     WLNQNAGPRDVVLECCRNEYDWPGRISAFTGLATLVAWDNSHEALWRSSQPALSAEIG
                     RRRQVVNAIYQGKDPDSPSELLTPQKLLDTLNTYGVDYVVVGAIERGTVGARTGAGYQ
                     PGEQVTPYGENVMKAALREVFRSSDGVTVVYGVQGAQVDANVVVPTPAPVVTPAEGGE
                     QADPNAPPQALFDRADPGPNRGQFNLPRGIARDAQGNFYVSDTENKRIQKFDPTGKWL
                     LSFGGEGTGNGQFSSISPGSIGTGPGGLAVDADGNVYVADTWNHRIQKFDSEGKFLMS
                     WGAFVNLSDPAAAADPQRD"
BASE COUNT          543 a         1115 c         1063 g          624 t
ORIGIN      
        1 tgggtgagac ttttctctgg tggttgacga cacaggtgct cggcctcgta gcccttccta
       61 tagcggcggt gctgatgcgg ggtgtgcccg atagagggta caccgccgcc aaggcgctgg
      121 gcctgctgct cgtcacgtgg ccggccttca ccattgccat gacgggcgtg gtgccgtacg
      181 ggcgcgtcct gctctttggc tgcctactgc tggtggcggc gctctcgacc tggttgctgc
      241 tgagagacaa ccgggcgctg ctgtgggagc ttcgcgcgca cttccgcacg cgctctctcg
      301 tgcggcacct ggtcgctgct gaagtgctgt tcgccgtgct attcgccttc tgggcgtgga
      361 tgcgggcctt caaccccgaa atcctcgacc aggagaagtt catggacttc ggcatcctga
      421 actccatact gcgcaccggc agcctccccc cgcccgatat gtggttcgcc gggcagtcgc
      481 tcaactatta ctatttcggc tacatcatca tagccgctat gtccagcctg agcggggtgg
      541 cggcctctgt gagcttcaac atcgccaacg ccttcctgtt cgccatgacg ggcctcgggg
      601 cctacggcgt ggtgtacaac ctcattgtgg gcaccatcct gcgccgcgcc ggacgcccca
      661 cgctcacgca ggccccgcca cccccacccc cggcaccttc gaccgcgagg ggccgccggg
      721 gcaacaaacc cgcacccgct cccgccaggc ccctgcccac gcggcaggtg caagcccaac
      781 aactggcgtt cgcgggtgcc acgtcaggta cctactccac ggtcgtggac gaggaggtag
      841 ccgcggcccc cctgcccgcc cgccggaaca gggcggccgc gcaacgtgac gaggcgcggt
      901 acgcggagcc tgtggaggcc ggcgagggca ccgacgaccc gactcgccgc gtgcccttct
      961 acctgtcccc catattgtac ggagtgctgg ccgctctcat gatcgtggcg atgggcaacc
     1021 tggccgcgcc tttcgggact aaggacgata ccaccgaaga agctaccatc aacggcaacg
     1081 gctggacttt ctgcttcttc tgcgccaaga aagattacga ttactggtgg gacccctctc
     1141 gtattatcct ggactaccgc accgtgcaac agccggatgg tagtatgctc aagtcaaagg
     1201 tcggctacga gaccatcaac gagttccccg ccttctcgtt cgtgctggcc gacatgcacc
     1261 ctcacgtcat ggggctgcct ttcgtgctgc tggcggtcat ggtggcttac gccctgggcc
     1321 gccgcagggt cgccggctcc aacctctggc gcgacggcct gccccgttcg ctcggcgggt
     1381 ggctggggct gctcctgtcc tccatcatcg tgggcgcgct ctacgccatc aacacctggg
     1441 actaccccac gtacttgttg gtgatgctgg gcggcctcgc cctgccctac atagcggcgg
     1501 gacaccggga ggagggtggc cggcgatggg cgcggccttt cgtcgtgcag gcgttcttgc
     1561 tcatcgcgat aagcttgctg gcctacctgc ccttccacct gactttcaag tcgttcgctg
     1621 gcggcggccc tgtggccttg cccgaaaaca tcgcaaacat acccgtgctg ggcgggctgt
     1681 tgcggagcct gagcgggctg ttcctggtga acaccgccga caagacgata acgggcttca
     1741 tggttatctt cggcatcttc ctgctctccc tcaccgtgtg gctcatttac gagttcgcca
     1801 cgtacatgcg caagcgcgcc cgcgccagcg aagaaggggt gaactcgctg ctggtgtttg
     1861 gtatcacgct catggtcatc ttgctggtgg ccttgtggac gcgcttcccg ctcctggctt
     1921 tgctgctacc cctgatcatc atggcgggct acctcatctg gcaggagccg cgccgcaccg
     1981 agcgtaacat ggtgctgggc gtcttcctgg tcgccgccac cattggcctg gggatagaga
     2041 tcatctacct gcacgacaac ttcggcaacc ggatgaacac cctgttcaag ttctactacc
     2101 agatgtgggt gctctgggcg cttgcggccg cttacggctt ctggcgcacg ttgcaggccg
     2161 cttttggcgc caaccgcctc atgagcgcgg gccgaggcta cgaccgggtg gagtgtgccg
     2221 tgaatgacac ccccaacggc ctgagagtgc tggcgggcac gtgggcggcg gtgttcctgt
     2281 tgctggtgct ctccgcgctt ccatacgcct ggttcggctc ccagcagagg aacatgatta
     2341 acaagacggc acccatcggt cttgacggca tggagacgtt gcggatgagg aatccaagcg
     2401 actacgaagc cgtcaactgg ctcaaccaga acgccggccc ccgcgacgtg gtgctcgagt
     2461 gctgccgcaa cgaatatgat tggccgggcc gcatctcggc ctttacgggg ctggccacgc
     2521 tcgtggcctg ggataactcg cacgaagcgc tgtggcgttc cagccaaccc gctttgtcgg
     2581 ccgagattgg ccgcaggcgg caggtggtca acgccatcta ccagggcaag gaccccgaca
     2641 gcccctctga gctactgacg ccccagaagc tgttggacac gctcaacacc tacggcgtgg
     2701 actacgtggt ggtcggcgcg atagagcggg gcacggtagg cgcacggacc ggcgccgggt
     2761 accagcccgg cgagcaagtc accccgtatg gcgagaacgt gatgaaggcg gcgctaaggg
     2821 aggtcttccg gtcgagcgac ggtgtgacgg tggtgtacgg ggtgcagggc gcgcaggtcg
     2881 atgcgaacgt ggtggtgccc acgcccgcgc ccgtggtgac tcccgccgag ggaggggagc
     2941 aagccgaccc caacgcaccg ccccaggccc ttttcgaccg cgcagaccca ggccccaacc
     3001 ggggccagtt caacctgcct cgcggcatcg ccagggacgc gcagggcaac ttctacgtgt
     3061 cggatacgga gaacaagcgc atccagaagt tcgaccctac cggcaaatgg ctcctctcgt
     3121 tcggcgggga gggcaccggc aacgggcagt tctcctcgat cagccccggc tccataggca
     3181 cggggccggg cggtctcgcg gtggacgccg acggcaacgt gtacgtggcc gatacctgga
     3241 accaccgcat ccagaagttc gactctgaag gcaagttcct gatgagctgg ggtgccttcg
     3301 tcaacctgtc ggaccctgca gcggcagccg acccgcagcg agata
//
LOCUS       DASRWZ010000735         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_127922, whole genome shotgun sequence.
ACCESSION   DASRWZ010000735 DASRWZ010000000
VERSION     DASRWZ010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127922"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2113
                     /locus_tag="VFH60_11650"
     CDS             <1..2113
                     /locus_tag="VFH60_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HET6314480.1"
                     /translation="LGLSQYDRLEEAAKEEARERKARMNELEGTIKQIDAQLLVRPEA
                     EKQLVEVEAQLIDAQESLATSRAELVELHAQVQSLDHNRDRLRELQERQRSREGMMLT
                     SKARLENNSARKSELVALIARRGEIEQGYADWQRVQEEERRLNGALATMRRMENEQSA
                     LERQIDGERIRLESTTTHHREQLRKLGSNLASRGVLESQLSEVLDKLRRFEQLQDRHE
                     DTRCMRDNLDVKMRTLKSELDACEEEGRQLAAKLKLIMTAHAEGQGHADCPLCGSDLT
                     ADALKRVQASYDHDIKQKRLEYERKRKELDAVNKEIVATQKKYAQEADDLKPLEMHRQ
                     REANLKASIAGLDMDEQQLAKEEAALVQVRMRLDEGDYAHEAREALLALQARMRSLAY
                     DEEAHRAVSRRVAEMRGQQYDQLYHKLQNAERELASVEQFIEDDTLHLEAWTAERAVD
                     AEEIGTLLPKLGQWEIVKQQHAEKLEEEKWLAGMVSRLGEQRGGLKKQIEHCEQQQEE
                     KGRYTTEYNKAAEEKAAYDELATAFGKKGIQAMIIENIIPEVEEEANKLLHRMTDGRM
                     TVQFATQRDAKSSKSVIETLDINISDEIGTRAYEMYSGGEAFRVNLAVRIALSKLLAR
                     RAGTQLQTLVIDEGFGSQDGQGREKLVSAIRSIEGDFERILVITHIEELKDEFPVRIN
                     IIKTPSGSHIMMNEGELAPAA"
     gene            2569..3408
                     /locus_tag="VFH60_11655"
     CDS             2569..3408
                     /locus_tag="VFH60_11655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6314481.1"
                     /translation="MKYAIYTPNFGPLGEPRVLAEMAREAEEAGWDGFFIWDHVLWTY
                     PENHPTYDPWVALAAIATATSRIRFGALVTPVARRRPWKLAREAVTLDHLSGGRLVLG
                     VGVGHDMFKEYSAFGESTDLKMHGEMLDEGLEVISGLCSGEPFSYEGKHYHIKDAHFI
                     PGPVQQPRIPVWVAGMWPHKKPFRRSARWDGIVPIGTTAALTPDDIRIIASYVQEHRT
                     SQEPFDVVCSGTPLAGSEDAVERAAAFEAAGATWWLEHFVPWGDRSVEEQRNVVRNGP
                     PVR"
BASE COUNT          787 a         1064 c         1150 g          548 t
ORIGIN      
        1 acttggcctg tcgcagtacg accgccttga ggaggccgcc aaggaagagg cccgcgagcg
       61 caaggcccgc atgaacgagc ttgagggtac gattaagcag atagacgccc agctactcgt
      121 gcggcccgag gccgagaagc agcttgtgga ggtcgaggcc cagcttatcg acgcccagga
      181 gagccttgct acctctcgcg ccgagcttgt agagctacac gcccaggtgc agagcctcga
      241 ccacaaccgc gacaggctga gggagttgca ggagcgccag cgcagccgcg aaggcatgat
      301 gctcacctcg aaggcgcgtc tggaaaacaa ctccgctcgc aagtccgagc ttgtggccct
      361 catcgcccgg cggggagaga tagagcaggg ttacgccgac tggcagcgcg tgcaggagga
      421 ggagcgccgc ctgaacggag cgctcgccac catgcgccgc atggagaacg agcagtcggc
      481 cctggagcgg caaatagacg gcgagcgcat caggcttgag agcaccacca cccaccaccg
      541 cgagcaactg cgcaagcttg gcagcaatct cgccagtaga ggcgtgctgg agagccagtt
      601 gtccgaggtg ctggacaagc taaggcgctt cgagcagcta caggaccggc acgaagacac
      661 ccgttgcatg cgggacaacc ttgacgtgaa aatgcgtacg ctgaagtcgg agctggacgc
      721 ctgcgaggaa gaaggccgcc agcttgccgc caagctcaag ctgatcatga ccgcccacgc
      781 cgaggggcag ggccatgccg attgtcccct gtgtgggagc gacctgaccg cggacgccct
      841 gaagcgcgtg caagcctcgt acgaccacga cataaagcag aaacggcttg agtacgaacg
      901 caagcgcaag gagcttgacg cggtaaacaa ggagatcgtt gccactcaga agaagtacgc
      961 ccaggaggcc gacgacctca agccccttga gatgcaccgc cagcgcgaag ccaacctgaa
     1021 agcgtctatc gcgggcctcg acatggacga gcagcagctt gcgaaggaag aggccgcgct
     1081 cgtgcaggtg cgcatgcggc tggacgaggg tgactacgct cacgaagcgc gggaggccct
     1141 gctggcgttg caggcccgca tgcgcagcct ggcctacgac gaggaggccc accgagcggt
     1201 cagccgccgg gtggccgaga tgcgcggcca gcagtacgac cagctatacc acaagctaca
     1261 gaacgccgag cgagagcttg cttccgtgga gcagttcatc gaggacgaca cgctgcacct
     1321 ggaggcctgg accgccgagc gggccgtgga cgccgaggag ataggcacgc tgctgcccaa
     1381 gctggggcag tgggagatag tgaagcagca gcacgccgag aagctagagg aggagaagtg
     1441 gctggccggg atggtcagtc gcctgggcga gcagcgcggc ggcctcaaga agcagataga
     1501 gcactgcgag cagcagcaag aggagaaggg ccgctacact accgagtaca acaaggccgc
     1561 tgaggagaaa gccgcctacg acgagcttgc caccgccttc ggcaagaagg gcatccaggc
     1621 gatgatcatc gagaacatca tcccggaggt agaggaggag gccaacaaac tgctccaccg
     1681 tatgaccgat ggccgtatga cggtgcagtt cgccacgcaa cgcgacgcca agtcgagcaa
     1741 gagcgtgata gagacgcttg acataaacat ctcagatgag ataggcaccc gtgcctacga
     1801 gatgtacagc ggcggcgaag cgttcagggt gaacctggca gtgcgcatcg ccctgagcaa
     1861 gctgctggcg aggcgtgcgg gcacgcagct gcaaacgcta gtgatagacg agggcttcgg
     1921 cagccaggac gggcagggcc gcgagaaact ggtcagcgcc attcgctcca tcgagggcga
     1981 cttcgagcgc atcctggtta tcacccacat agaggagcta aaggacgagt tccccgtgcg
     2041 catcaacatc atcaagaccc ccagtggctc ccacatcatg atgaacgaag gcgaactcgc
     2101 ccccgccgcc tagcctgtta ggagacggaa cggccaagag ctccaagaga cgaaaacgca
     2161 aagacgcaga ggacgcaaag aacgcgcaaa gattaggatt gtttagtgag tacaaaagga
     2221 ccagggtaca tcatgtcgta ccctggtcct tttgcctggg acaccaccct aaactctgcg
     2281 cgccctctgc gtcctctgcg tctctgccgt gaaccgtact taatattctt ggcgctcccc
     2341 tggcgctctt ggcgtcttgg cggttccgtc tcttagtggt cgtttgaacc atctagggct
     2401 gccccggagt atcttagttg tagcaactca ccatgaagcc gggtgctttc tatgctgcga
     2461 gcgggttggg gtcaatgcac agactgcacc gaggggatgg gccgcacacc attcgttgcg
     2521 aggtgaggcc ctgctgtgtt atcagcaccc taaaaggagc gcttcaggat gaaatatgcg
     2581 atttacaccc ctaactttgg gccgttgggc gaaccgcgcg tgcttgccga gatggcacgt
     2641 gaggcggaag aagccggttg ggacggcttt ttcatctggg accacgtact ctggacctac
     2701 cccgaaaacc acccgaccta cgacccctgg gtggccctcg ccgctattgc caccgccacc
     2761 agccgcatca ggtttggcgc gctcgttacc ccggtcgccc gtcgtagacc gtggaagctg
     2821 gcccgcgaag ccgtgacgct ggaccacctg tccggcggac gcctagtgct cggcgtgggc
     2881 gtcgggcacg acatgttcaa ggagtacagc gccttcgggg agtctacgga cctcaaaatg
     2941 cacggggaga tgctggacga gggcctggaa gtgatttcag ggctgtgctc gggcgagccg
     3001 ttcagctacg agggcaagca ctaccacata aaggacgccc acttcattcc cggcccggtg
     3061 cagcagccgc ggataccggt ctgggtggcc gggatgtggc cccacaagaa gccgtttcga
     3121 cgttcggccc ggtgggatgg catcgtgccg ataggaacca ccgcggcgct tacccccgac
     3181 gatatacgca tcatcgccag ctacgtccag gaacaccgca cgagccagga gccgttcgac
     3241 gtcgtgtgca gcggtacgcc actcgcgggc agtgaggacg cggtggagag agcagccgcg
     3301 ttcgaggccg ccggggccac gtggtggcta gagcactttg tgccctgggg cgaccgctcc
     3361 gtcgaggagc aacgcaacgt ggtacgcaac ggccctcccg tcaggtagcg tgcctgtagg
     3421 caaccttcct gctacacgct gaatccctca ggcagtccct atgtcacgtt tgcgggacca
     3481 tgttcccctc tgtcattctg agcgtagcga agaatctaaa gaagctggta cagggaggct
     3541 gttgaacaa
//
LOCUS       DASRWZ010000736         4711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_676076, whole genome shotgun sequence.
ACCESSION   DASRWZ010000736 DASRWZ010000000
VERSION     DASRWZ010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676076"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..306
                     /gene="rpmH"
                     /locus_tag="VFH60_11660"
     CDS             145..306
                     /gene="rpmH"
                     /locus_tag="VFH60_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET6314482.1"
                     /translation="MVKRTYQPKRIPRKREHGFLARMRTKGGRRVVSARRAKGRWRLT
                     VSDEAKGVK"
     gene            386..886
                     /gene="rnpA"
                     /locus_tag="VFH60_11665"
     CDS             386..886
                     /gene="rnpA"
                     /locus_tag="VFH60_11665"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET6314483.1"
                     /translation="METLKRSGQFERVRREGRTWAGGLMVLNAATNGQDAVRCGFIAG
                     KKVGGAVERNRARRLLREALRARLPRIKPGFDLVLIARAPIVQVKLDAVGKELDDLLL
                     RGKLLLPPAETAGAQSRIIGSKGDPASQGAGTPLPGTERTTVETPERAYTVGTVGTAD
                     LPETRT"
     gene            896..1135
                     /gene="yidD"
                     /locus_tag="VFH60_11670"
     CDS             896..1135
                     /gene="yidD"
                     /locus_tag="VFH60_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET6314484.1"
                     /translation="MSIAKLGRLPRQPLLWLIRLYQRTVSLALPPNTCRFYPSCSYYG
                     YEAIEKYGIIKGGAMATWRIMRCNPWNDGGFDPVK"
     gene            1222..2544
                     /locus_tag="VFH60_11675"
     CDS             1222..2544
                     /locus_tag="VFH60_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HET6314485.1"
                     /translation="MEIFGFLWNSFWHVLLNVLVAINSVVPNAGLSIIVFTLLMRLLT
                     VPLTMKALRSSRNMQQIQPLIKEVQRKHGKDRAKQQEELMKIYAEYGINPAAACFPML
                     IQLPIFIGLYSALTFVLPVFSGSADEIAKATAAHLEQLKAILWNPAWVDNAANFGQAF
                     LWVPNLAREDPLHIWPVLSGVFQFIQSRMAMPVRDPNQPLDPQTRMMNNMMQFMPIYI
                     IFISWGFPAGTVIYWAFSSIFGAVQQYFITGFGTLPDLPGLGWLPRKPIQPPTPIHPR
                     GENGELPTGARPQPKRGMMTWMMEKAAAAQETQRAAQGAQGAQATTAIEERRGGAATR
                     PTGASTVKTGSANGGEPTRKTGSVLRGGNVGRDAIENGAAGQRSKGRGSVYGSAQADD
                     GETDGETPRGAAKYASDLDLVEGTAGAASARSGGAGPAQLPRKRKNKR"
     gene            2638..3723
                     /locus_tag="VFH60_11680"
     CDS             2638..3723
                     /locus_tag="VFH60_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HET6314486.1"
                     /translation="MRQVDIQARSVDEAVRLALEQLGRTRDQVKVEVLATDPGGDEVL
                     VRVTAPDLPGASSQEEVSPRGRRGFEDRGGARPAERPERPTDDIEPGNRDDRGVRPDR
                     DRGPRPDRPPGGGYAGRERLTGSRSGGGGSGGGVDRGGRQDRGGMGMGGGMGRTYGRP
                     EGGRYRTEPQYDPDVPGEIEIDSSLQMEAAPEGEEGAAKTNVAPLAAEVLQTILLGMK
                     IRGRIVRRMPAEEGDAAFEEDQDSLVLNVVDLNERDQNTLVGRRGETLDAIQFLVNLV
                     LSKQTDHWARVTVDVEGYRLRRKHSLINLARRTADQVETRGQAVPLEAMPPHERRIIH
                     MTLADHPSVTTESSGAEPERRVVVLPK"
     gene            3824..4699
                     /locus_tag="VFH60_11685"
     CDS             3824..4699
                     /locus_tag="VFH60_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6314487.1"
                     /translation="MSDDHLHLRPDYLVLGHVARDVLPGGDGTAGGTVTYSSLAAQKL
                     GLQAAVVTACAPEDEGLLAPLREAGVWVHALPSPVTTTFSNTYDTTGRRVQVIGGQAM
                     TLDLGDVPEVWRTAGIAHLGPIAQELPPELARHLSPRRLLGVTPQGWLRSWDEQGRVT
                     QSGTPVPPALIRLPRSAVLVLSIEDLGYSPDLLFSYVKLTKLTIVTRGVEPAHVYRHG
                     TLTQAPAMPARVVDPTGAGDVFAAAFFVRYAETHDAAQAARFAHAAAACAIEGHGTSA
                     IADRATVERRLQGQT"
BASE COUNT          957 a         1479 c         1546 g          729 t
ORIGIN      
        1 cagagcaact aacaaccaac aactaacaac tgataactcg tttgacacgc ctgggagcat
       61 cacgtataat ggcgaagtac attttatact ggcgtattgt gccgtccgca ggcggcagtt
      121 caaaaatagg agaaacacga caggatggtc aagcgtactt accaaccaaa gcgcatcccg
      181 cgcaagcgcg agcacggttt tctcgcccgc atgcgcacca agggtggccg ccgcgtagtc
      241 tcggctcgtc gcgccaaggg ccggtggcgg ctcactgtca gcgacgaggc taagggcgta
      301 aagtagagag tgatgaggac tgaggactga ggactgagtg gtttgagact gctcggtcct
      361 cagtcctcag tcctcagtcc tgactgtgga gacactcaag cgtagcgggc agttcgagcg
      421 agtcaggcgg gaggggcgca cctgggccgg gggccttatg gtgctgaacg ccgccactaa
      481 cgggcaggac gccgtgcgct gtgggttcat cgcaggcaag aaagtagggg gagcggtcga
      541 gcgcaacagg gcacgccgct tgctccgtga ggctctcagg gcaaggctgc cgcgcatcaa
      601 accggggttc gacctggtct tgatagcccg cgctcctatc gtacaggtga agctcgacgc
      661 ggtaggtaag gagctggacg acttgctgct acgaggcaag ctactgctcc caccggctga
      721 aaccgccggg gcgcaatcgc gtataatagg aagtaagggc gacccagcct cgcaaggcgc
      781 gggcaccccc ctacccggaa ccgagcggac cacggtggaa acaccggaga gagcatacac
      841 cgtcggcacg gtcggcacgg cggacctgcc ggaaacaagg acataggcac cagagatgag
      901 catcgccaag ctcggaaggc tgccgagaca gccccttctg tggctaatac gcctctacca
      961 gcgcaccgtc tccctggcgc taccgcccaa tacctgccgt ttctatccta gctgctcgta
     1021 ctacggctac gaagcgattg agaagtacgg catcatcaag ggcggagcta tggctacctg
     1081 gcgcatcatg cgctgcaatc cctggaacga cggcggcttc gacccagtga aataacgatt
     1141 tggaatttcg gatttcgaat ttcgaatttg ctggatacct gctaaattcg aaattcgaaa
     1201 ttccaaatcc cggagattgt cttggaaata tttggcttct tgtggaactc gttctggcac
     1261 gtactgctga atgtgctggt ggcgatcaac tcggtggtgc cgaacgcagg cctgtctatc
     1321 atcgtcttca cgctgttgat gaggctgctc accgtgccgc tcactatgaa ggcgctgcgg
     1381 tcgtcgcgca acatgcagca aattcagccg ctcatcaaag aggtccagag gaagcacggc
     1441 aaggaccggg cgaagcagca agaagagctg atgaagatct acgccgagta tggcataaac
     1501 ccggcggcag cctgcttccc gatgctgata cagctgccta tcttcatcgg cctgtacagc
     1561 gcgctcacct tcgtgttgcc cgtcttcagc ggcagcgcgg acgaaatcgc gaaagccacg
     1621 gccgctcacc tcgagcagct caaggccata ctctggaacc ccgcctgggt cgataacgcc
     1681 gccaacttcg ggcaagcctt cctgtgggtg cccaacctgg cccgcgaaga ccccctgcac
     1741 atctggccgg tcctctccgg cgtcttccag ttcatccaga gccgcatggc catgccggtg
     1801 cgcgacccca accagccgct cgacccgcag accaggatga tgaataacat gatgcagttc
     1861 atgcctattt acatcatctt catatcgtgg ggcttcccgg cgggcacggt catctactgg
     1921 gcgttctcga gcatcttcgg cgcggtgcag cagtacttca ttaccggctt cggcaccctg
     1981 cccgacctgc ccggcctggg ttggctgcct cgcaagccca ttcagccgcc cacgcccatc
     2041 cacccccgcg gtgagaatgg tgagcttcct actggggcca ggccgcagcc caagcggggc
     2101 atgatgacct ggatgatgga gaaagcggcg gcggcccagg agacgcagag agcggcccag
     2161 ggagcgcagg gagcgcaggc caccacggct attgaggaga ggcgcggcgg ggcggccaca
     2221 aggcccaccg gcgcgagcac cgtcaagacc ggcagcgcga acggcggcga gccgaccagg
     2281 aaaacaggct ccgtgttgcg cggaggcaac gtcgggcgcg acgcaataga gaacggcgcg
     2341 gcgggacagc gcagcaaggg ccgaggcagc gtctacggca gcgcacaggc cgatgacggc
     2401 gaaacggacg gcgaaacgcc ccgtggcgcg gccaaatacg catcggacct cgaccttgta
     2461 gaaggcacag cgggcgcggc gtccgccaga agcggcgggg cgggtccggc ccaactccca
     2521 cgcaagagaa agaacaaaag gtagtgatga ggactgagga ctgagtgatg agtactcaag
     2581 tgaccgctca tcactcagtc ctcagtcctc agtcctcaac gctaggagga aacgagtttg
     2641 cgacaggttg atatacaggc gcgcagcgtt gacgaagcgg tcaggctggc ccttgagcaa
     2701 ctgggacgca cgcgagacca ggtaaaggta gaagtgctcg cgaccgatcc cggaggagac
     2761 gaggttcttg taagggtgac cgctcccgac ctgccggggg cctcatccca ggaggaagtt
     2821 tcgccgaggg gccgcagggg gttcgaggac aggggtggcg cgcgccccgc cgagcgtccc
     2881 gagcgtccca ccgacgacat cgagcctggc aatcgagacg accgtggcgt acgtccagac
     2941 agggaccgtg ggcctcgccc tgaccgcccg ccgggtggag gttacgccgg cagagaacgg
     3001 ctgaccggga gtcgtagcgg agggggagga agtggaggcg gggtagaccg gggcggcagg
     3061 caggaccgtg gcggcatggg catgggtggc ggcatgggcc ggacctatgg tcgcccggaa
     3121 ggcggccgct accgcaccga acctcagtac gaccccgacg tgcccggcga gatcgagatt
     3181 gatagctcgc tgcaaatgga agcggcgccg gaaggcgagg agggggccgc taagaccaac
     3241 gtcgcgccgc tcgccgccga agtgttgcag actatcctgc tcggcatgaa gatccggggc
     3301 cgcatcgtgc gccgaatgcc cgccgaggag ggggacgccg ccttcgagga ggaccaggac
     3361 tcgctggtgc tcaacgtggt tgacctcaac gagcgcgacc agaacaccct cgtaggccgc
     3421 cgtggggaga cgctggacgc gatacaattc ctggtgaacc tcgtcttgag caagcagacc
     3481 gaccactggg cgcgggtcac cgtggacgtc gagggctaca ggctgcgccg caagcattcg
     3541 ctcatcaacc tcgctcgccg caccgccgac caggtggaga cgcgaggtca ggcggtcccc
     3601 ctggaagcga tgcccccgca cgagcgccgc atcattcaca tgacgctggc cgaccacccg
     3661 agtgtgacca ccgagtcgtc cggggccgag cctgagcgcc gggtagtggt gctgccgaag
     3721 taaggcagat gcgagctata cagacctttg aaggggcggc tatatgctgc cccttcacgc
     3781 tttgcgcggc taaccgcaag atgctctacc aagaggcagg ctcatgagcg acgaccatct
     3841 ccacctgcgc cccgactatt tggtcctcgg ccacgtcgcc agggatgtgc tgccgggcgg
     3901 tgacggcacc gctggcggca ccgtcaccta ctcgtcgctg gcggcccaaa agctgggctt
     3961 gcaggcggcg gtggtcactg cgtgcgcgcc ggaggacgaa gggctgcttg cgcccctgag
     4021 ggaagcgggg gtgtgggtgc atgccctgcc ctcccccgtc actaccacct tcagcaacac
     4081 ctacgacacc acgggccgcc gcgtgcaggt aatcggcggg caggccatga cgctcgacct
     4141 gggcgacgtg ccggaggtgt ggcgcactgc cggaatcgcg cacctggggc ctatcgcgca
     4201 ggagcttccc cctgagctgg cgaggcacct ctcgcccaga cggctgctgg gggtcacgcc
     4261 gcagggttgg ctgcgctcct gggacgagca aggccgcgtc acccagtcgg ggacacccgt
     4321 gcctcccgcc ctgatacgcc tgccgaggag cgccgtgctc gtgctcagca tcgaggacct
     4381 gggatacagc ccggacctgc ttttctctta tgtaaagctg acaaagctca cgatagtcac
     4441 aagaggagtg gaaccggcgc acgtataccg gcacggcacc ctcacgcaag cgcctgccat
     4501 gcccgcgcgg gtagtggacc ccacgggcgc gggggacgta ttcgccgccg ccttcttcgt
     4561 ccgctacgcc gagacgcacg atgccgcgca agcggcccgt ttcgcccacg ccgccgccgc
     4621 ctgcgcgata gagggccacg gcacctcggc catcgcggac agggcaacgg tcgagcgaag
     4681 gcttcaaggc caaacgtaag gcggcttgcc a
//
LOCUS       DASRWZ010000737         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1018160, whole genome shotgun sequence.
ACCESSION   DASRWZ010000737 DASRWZ010000000
VERSION     DASRWZ010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018160"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VFH60_11690"
     CDS             complement(<1..460)
                     /locus_tag="VFH60_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6314488.1"
                     /translation="MINRAIELAARAHRDQVRKGTDIPYVAHPYAVGMSLARAGCDDE
                     VVAAGILHDTVEDTDVTLEELRAQFGARIASIVEGCSEPERGKSWEERKEHTLAYLPT
                     APYDIRLVSLADKLHNARSILADFGASGEGAWSRFKRGRPQQEWYYRGLAE"
     gene            727..2880
                     /gene="rhaD"
                     /locus_tag="VFH60_11695"
     CDS             727..2880
                     /gene="rhaD"
                     /locus_tag="VFH60_11695"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF006189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HET6314489.1"
                     /translation="MQSGTEAIIENRWSDDETRGMDDLDLLVYQSRLVGSDPRLVVWG
                     GGNTSIKTHETDFRGRPGMVLRVKGSGSDLKAVVRSDFPGVRMDDVLPLMDRDDMSDE
                     EMVEYLGMTLMEPGSPRPSIETLLHAFVPARSVVHSHADAILALTNNARPGEALAEVF
                     GGELAIVPYRRPGFLLSKQVGEAVRDNPGIRGVILLNHGLITWDDEPGEAYRLHIEMV
                     DRAARYAKARGMAPTATSHGTRLADAERLEAAAKLAPVLRGLLGQDKRVIVRYHDSPE
                     VLRFVSGDIVPLDRMREVLRQGAATPDHILNTKRTPLWIEAGAGSDAEGLARAARAAY
                     EEWVSDYKQYYDAHQRGEGMLPVVPRVVLVAGVGMFSVGKDSRAATISSDIYHHTIGI
                     IEAAEGLGGYRSLSLKDAFAAEYWPLELYKLTLAPPEKALSRRVVLVTGAAGAIGAGI
                     ARRFAAEGAHVVCADLDLDKSGALAAELSKANPANRSMAVSMDVTDESSVAGAYKQAV
                     LEYGGVDVVVSNAGIAKSCPIDELSLEDWEGSFAVNARGHFLVAREALRVMKAQGTGG
                     SFVFIATKNVTAPGKEFAAYSASKAAEAQLARVLALEGAPYGIRSNMVNPDAVFAGSG
                     LWSQEVRQQRARAQGIEPAEIEEHYRKRNLLQVEVTAADVAEAALFYASDRSAKTTGS
                     MLPVDGGLREAFPRLWCRGYSPDGGTPLTGAGGVP"
BASE COUNT          535 a          896 c          991 g          494 t
ORIGIN      
        1 cttccgccag cccgcggtag taccactcct gctgagggcg gccccgcttg aagcgcgacc
       61 acgcgccctc gccgctagct ccgaaatcgg ccaggatgct acgggcgttg tgtagcttgt
      121 cggccagtga caccagccgg atgtcatacg gggcggtagg taggtaggcc aacgtgtgct
      181 ccttgcgctc ctcccaactc ttccccctct ccggctcaga gcagccctcc acaatagagg
      241 caatccgcgc gccgaattgc gcgcgcagct cctccagcgt gacgtcggta tcctccaccg
      301 tgtcgtgcag gatgcccgcg gccaccactt catcgtcgca gcccgcgcga gccagcgaca
      361 tgcccaccgc gtaagggtgc gctacgtacg gaatgtcggt gcccttgcgg acttggtccc
      421 ggtgtgccct ggccgcaagc tcaatagccc ggtttatcat gcgtcctccg tatgggtagg
      481 gggcgaagtg gatcatgcca tgtctgttag ttacacgcct cattttatca tgacatcgcc
      541 cggcggttga aacagcgtgc aacagctaca aagtccccct tcggggactt ctcgcatctg
      601 aggtagtccc tgaaggggga ctttgtaatc ctagcccgtg actttagtcg ccgggtactt
      661 ctataatgtg aatggcgacg ttctaattat cggatacgcc acccatcttg ataaccgagg
      721 caagttatgc agagcggcac tgaagctata atcgaaaaca ggtggtcgga cgacgaaacg
      781 cgcggtatgg acgacctgga cctgctggtg taccaatcgc ggctggtggg ttccgacccc
      841 cggctggtag tgtggggcgg gggcaacacc tccatcaaga cgcacgagac cgacttccgg
      901 gggcgacccg gcatggtttt gcgcgtgaag ggcagcggtt cggacctgaa ggccgtggtc
      961 cgcagcgact tcccaggggt gcgcatggac gacgtgctcc ccctcatgga ccgcgacgac
     1021 atgtccgatg aggagatggt ggagtacctc ggcatgacgc tgatggagcc ggggtcgccc
     1081 cgcccctcca tcgagacgct gctgcacgcc ttcgtgcccg cccgtagcgt ggtgcacagc
     1141 cacgccgacg ccatactggc cctcaccaac aacgcccgcc ctggggaggc gctggccgag
     1201 gtgttcggcg gcgagctggc tatcgttccg taccggcggc ccggcttcct cctgtcgaag
     1261 caggtgggcg aggcggtgcg ggacaatcca ggcatcagag gggtcatcct gctcaaccac
     1321 ggcctcatta cctgggacga cgagccgggg gaagcctacc gcctgcacat agagatggtg
     1381 gacagggccg cacgctacgc aaaggcgcgg ggcatggcac ccactgcaac cagccacggc
     1441 accaggctag cagatgcgga aaggctggag gcagcagcca aattagcccc ggtgttgcgc
     1501 ggcttgctgg ggcaggacaa gagggtaatc gtgcgctatc acgactcccc agaggtccta
     1561 cgcttcgtat cgggcgacat tgtgcccctc gaccgcatgc gcgaggtgct tcgacaaggg
     1621 gcggccactc ccgaccacat cctcaacacg aagcgcactc ccctgtggat agaagccggc
     1681 gcggggtcgg acgctgaagg tcttgcgcgg gcggcgcggg cggcttacga agagtgggtg
     1741 agcgattaca agcagtacta cgatgctcat cagaggggag aggggatgct gcccgtcgtg
     1801 cccagggtgg tgctggtcgc gggggtaggc atgttctcgg tgggcaagga cagccgcgcc
     1861 gcaaccattt cgagcgacat ctaccaccac acgatcggca taatcgaagc ggcggaaggg
     1921 cttgggggct accggtcgct gtcgctaaaa gatgcgtttg ccgccgagta ctggccgctg
     1981 gaactgtaca agctaaccct ggccccaccg gagaaagccc tcagccgccg ggtggtgctg
     2041 gtcacggggg cggcgggggc tatcggggcc ggtattgcgc gacggttcgc ggcggaaggc
     2101 gctcacgtgg tatgcgccga cctggacctg gacaagtcgg gcgcgctggc agccgaattg
     2161 agcaaggcaa accccgccaa caggtctatg gcggtgagca tggacgtgac ggacgagtca
     2221 tcggtggcag gggcgtacaa gcaggccgtg ctcgaatacg ggggcgtgga cgtggtggtg
     2281 tcaaacgcgg gcatagccaa atcgtgcccc atcgacgagt tgagcctgga ggactgggag
     2341 ggtagcttcg cggtgaacgc cagggggcac ttcctcgtgg cccgcgaggc gttgcgcgtg
     2401 atgaaggcac agggcacggg gggcagcttc gtgttcatcg ccaccaagaa cgtcaccgcg
     2461 cccggcaagg agttcgcggc ctacagcgcg tcaaaggcag ccgaggcaca actggcgcgg
     2521 gtgctggcat tggagggggc accttacggc atccgctcca acatggtcaa ccccgacgcg
     2581 gtgtttgccg ggtccggtct ctggtcgcag gaagtaaggc agcagcgggc cagggcgcag
     2641 ggtatcgagc cggcggagat agaggagcat taccgcaagc gcaacctcct gcaggtggaa
     2701 gtcaccgccg ccgacgtagc cgaggctgcc ctcttctacg cctccgaccg ctcggccaag
     2761 actaccggga gcatgctgcc cgtagacggg ggcttgcgag aggcgttccc ccgcttatgg
     2821 tgcagggggt actcccctga cgggggtact ccccttacgg gggctggggg tgtcccctag
     2881 tgcgctctcc ctcacaaggg ggtgcagggg tactcc
//
LOCUS       DASRWZ010000738         3749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_127994, whole genome shotgun sequence.
ACCESSION   DASRWZ010000738 DASRWZ010000000
VERSION     DASRWZ010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127994"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VFH60_11700"
     CDS             <1..1239
                     /locus_tag="VFH60_11700"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6314490.1"
                     /translation="TRYSALGTRHSALGTRYSHLMKIALDYTTGIYPGAGIARYTRSL
                     VGALAELDGENSYRLFYAARGLPRPTPETRQAAALFRRHPNFRPARVPMSVRQMFAVW
                     QRLRAPLPVELFAGRIDLLHSPDFVSPPHLWGIDVITVHDLSFMVVPECAEPSLASFL
                     GKTVPRAVRRADHIIAVSRQTRDDLVKLLSVPPGKVTVAYNGVDARFVPGAGVSRAGE
                     LRASLGLPGRFVLHVGTLEPRKNLARLVEAYGLLLERATDGSGLRDVGLVLAGRRGWL
                     YEPVLAAAERANAAGGRVVLVDYVRDDDLPTLYNMAAAFAYPSLYEGFGLPAAEALAC
                     GVPTLVSRDGALAEVVGEAALKVDAYSVEAIAGGLKKLLTDQGLRTHLADAGPQQVAQ
                     FTWQAAARTVLDVYTKSRVG"
     gene            1457..2047
                     /locus_tag="VFH60_11705"
     CDS             1457..2047
                     /locus_tag="VFH60_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314491.1"
                     /translation="MNEDNSKLKTQNHALGRSEGSSLPEDPFDRLYERWAEHALRKHL
                     DPPSRERWDEIVLRLISSEDPERVRLYRRIEAAGDDTLGGDAYGLWSKMPLYTTNRTV
                     PLKTLPELAEYATGHHGSMRRLMEAKGLGEKYHAIVTAQKPKRSRFSIDPDDPADKPR
                     VRRSQMSVLAISCAGMLALILMVLTALLLIAIGMTS"
     gene            2054..3646
                     /locus_tag="VFH60_11710"
     CDS             2054..3646
                     /locus_tag="VFH60_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HET6314492.1"
                     /translation="MLEQQAPSPDAQPLGTQQAALGTPSAARRVALNAFNPFAAQLFT
                     KLLLMGYGMVQYRLFAQDQAALDGYILAGVVFSYTSTISEWGMGTLVPRDVARSRGTD
                     AEAEVAAALFGRSLTLRLLISLGLFVPVAALIAVYTTFFNLRADGAWAVAILSLSLIP
                     SAFSGSVTALLYAYERMSLPAGIGIATVVLNVALGLSALALGLGVLGLALSAFIATLI
                     TALAFLWVLRRNFPGVAVRLGRLSLRGREARSLLGAGWPLMLNALLVGLFFRVDIFII
                     KAAPGGEGDLTRYNTAYSFLGFVLLISPAVTLALFPRMARHAVSDRPRLNSEYTFALK
                     MMLVISVPIVAGTVWLAPLLITVLTLDAPNYLPDAAIALRILIFFLPLSFVNGLTQYV
                     LIAVDRQRLITRAFGVTVLFNLLANLLLIPVLGIYGAALTTILSEVVLLVPFIMWTRR
                     EVGDVPLLSISVKPALAGGAVALLVWLLWPVQEAWRSGWGTFALYLAIALAIPLVYVA
                     VLMALRPFTAPETQTLRGLLRR"
BASE COUNT          607 a         1272 c         1214 g          656 t
ORIGIN      
        1 actcggtact cggcactcgg cactcggcac tcggcactcg gcactcggta ctcgcatctc
       61 atgaagattg cactcgatta caccacaggc atatatccgg gagcggggat cgcgcggtat
      121 acgcggtcgc tggtcggggc gttggcggag ctggacgggg agaatagcta caggttgttt
      181 tacgcggcca gggggctgcc ccggcccacg cctgagacgc ggcaggccgc ggccctcttc
      241 cggcggcacc ccaacttccg gcccgcgcgg gtgcctatgt cggtgcggca gatgttcgcg
      301 gtgtggcagc gtctgcgcgc gcccttgccc gtggaactgt tcgcggggcg catagacctg
      361 ctgcattcgc ccgatttcgt gtctcctcct cacctgtggg gcatcgatgt aatcaccgtg
      421 cacgacctct cgttcatggt cgtgccggaa tgcgccgagc caagcctggc ctccttcctc
      481 ggcaagacgg tgccacgtgc cgtgcgccgg gccgaccaca tcatcgccgt ttcccgccag
      541 acccgcgacg acctggtgaa gctgctgtcc gtgccacccg gcaaggtcac ggtagcctac
      601 aacggcgtgg acgcgcggtt cgtgcccggc gcgggcgtga gcagggcggg cgaactgagg
      661 gccagtctgg gcttgcctgg aaggttcgta ctgcacgtgg gcacgctcga gcctcgcaag
      721 aacctggcga ggctagtgga ggcgtacggc ctgctcctgg agcgcgccac cgatggtagc
      781 gggctgcgag acgtggggct ggtgctggcg ggccgccggg gctggctgta cgagccggtg
      841 ctggcagccg ccgagcgggc gaacgcggcg ggcgggcgcg tggtgctggt cgattacgtg
      901 cgcgacgacg acctgcccac cctgtacaac atggcggcag ccttcgctta cccaagcctc
      961 tatgaggggt tcggcctgcc cgccgctgaa gccctcgcgt gcggcgtgcc caccctggtg
     1021 tcgcgggacg gcgcgctcgc ggaggtggtg ggtgaggccg ccctcaaggt ggacgcctac
     1081 tccgtagagg caatagcggg tggcctgaaa aagctgctga cggaccaggg gttgcgcacc
     1141 cacctggcag acgccgggcc gcagcaggtg gcccagttca cctggcaggc agccgcacgc
     1201 acggtgctgg acgtgtatac aaagtccagg gtcggctaaa ccaggcacct gggtgcaggg
     1261 ataagcagta tgggcctgag cacgcataaa cgtgacaacc ggcgcgggct tgatccggct
     1321 gctagcccca ggactgcgta ccgtccatga gcaagcttac gcgcctgtga gtcagggcta
     1381 ccgtctctgt gtaaacggga aagctaggcg gtggctggta tagtatatcc agcaaccgta
     1441 gcggggcaaa cccaccttga acgaagacaa ctcaaaactc aaaactcaga accacgccct
     1501 tgggcgaagc gaagggtcat cacttccaga ggaccctttc gaccgcctct acgagcggtg
     1561 ggccgagcac gccctccgca agcacctcga ccccccttcg cgcgagcggt gggacgagat
     1621 agtgctgcgc ctcatctcca gcgaagaccc ggagcgggtg aggctctaca ggcggataga
     1681 ggccgcggga gacgacacgc tgggcggcga tgcttacggc ctgtggagca agatgcccct
     1741 ctacaccacc aaccgtaccg tgccactgaa gaccctgccc gaactcgcgg agtacgccac
     1801 agggcatcac ggctccatgc ggcggctgat ggaggcgaag gggctaggcg agaaatacca
     1861 cgcgatcgta accgcccaaa agccgaagcg ctcccgtttc tccatcgacc ccgacgaccc
     1921 agccgataag ccccgcgtca ggcgttcgca gatgagcgtg ctggccataa gctgcgctgg
     1981 catgctggcc ctcatactga tggtcctcac cgccctgctc ctcatcgcga tagggatgac
     2041 cagctagccc gccatgctcg aacagcaagc cccctccccc gacgcccagc cgctcggcac
     2101 ccagcaagcg gccctcggca cccccagcgc cgcccgccgc gtggccctca acgccttcaa
     2161 ccccttcgcc gcccagcttt tcaccaagct gctgctgatg ggctacggca tggtgcagta
     2221 caggctgttc gcccaggacc aggcggcgct cgacggctac atactcgcgg gggtggtctt
     2281 ttcctacacc agcaccatct cagagtgggg catgggcacg ctcgtgccga gggacgttgc
     2341 caggagccgg ggcacggacg ccgaggcgga ggtggcagcc gcgctgttcg gcaggtcgct
     2401 caccctgcgc ctgctcatca gcctgggcct tttcgtgccc gtggcggccc tcatcgcggt
     2461 ctataccacg ttcttcaacc tgcgggccga cggggcgtgg gccgtggcga ttctcagcct
     2521 gtccctcatc ccaagcgcct tctccggctc ggtgaccgcg ctgctctacg cctacgagcg
     2581 gatgtcgctc ccggcgggca taggcatcgc caccgtggtg ctgaacgtcg cgcttgggct
     2641 gtcggccctg gcgctggggc tgggcgtgtt ggggctggcg ctgtcggcct tcattgctac
     2701 gctaatcacc gccctggcgt tcctgtgggt gctgcggcgc aacttccccg gcgtcgcggt
     2761 gaggctgggt aggctttcgc tccgagggcg tgaggcgcgg tcgctgctgg gggcgggttg
     2821 gccgctcatg ctcaacgcgc tgctggtggg ccttttcttc cgggtcgata ttttcatcat
     2881 caaggccgcg ccgggaggag agggcgacct cacccgctac aataccgcct acagcttcct
     2941 cggcttcgtg ctgctgatta gccccgccgt caccctggcc ctcttcccgc gcatggcccg
     3001 ccacgccgta tcggaccgcc cccgcctcaa tagcgagtac accttcgccc tcaagatgat
     3061 gctggttatc tcggtgccca tcgtggccgg gacggtgtgg ctcgccccct tgctcatcac
     3121 ggtactgacc ctcgacgcgc ccaactacct gcccgacgcc gcaatagccc tgcgcatcct
     3181 gattttcttc ctgcccctca gcttcgtcaa cggcctcacc cagtacgtgc tcatagccgt
     3241 ggaccgccag cgcctcatca cgcgggcttt cggcgttacg gtgctgttca acctgctcgc
     3301 caacctgctg ctgataccag ttttgggcat ctatggcgcg gccctgacta ccatactcag
     3361 cgaagtcgtg ctcctggtgc cgttcatcat gtggacccgg cgagaggtgg gagacgtgcc
     3421 gctgctctcc atttcggtga agcccgcgct ggcgggtggg gccgtggccc tgctggtgtg
     3481 gctgttgtgg cccgtgcagg aggcgtggcg cagcggctgg ggcactttcg ccctgtactt
     3541 ggctatcgcg ctcgccatcc ccctcgtcta cgtcgcggtg ctgatggccc ttcgcccctt
     3601 caccgccccc gaaacgcaaa cgctcagagg cttgctacgc cgataaggag acggaaccac
     3661 caggacgcca agaacgccaa gagcgcgcca agagacgaaa acgcaaagca tgccctgagc
     3721 gtagtcgaag ggacgcaaag gacgcaaag
//
LOCUS       DASRWZ010000739         5424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1360778, whole genome shotgun sequence.
ACCESSION   DASRWZ010000739 DASRWZ010000000
VERSION     DASRWZ010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360778"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2117)
                     /locus_tag="VFH60_11715"
     CDS             complement(<1..2117)
                     /locus_tag="VFH60_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6314493.1"
                     /translation="MALSTQSASIGADWLPVPAPADMANPSAGWWLSILERALISRAM
                     DDLEVTKEYRPNPDKPLEGKLKFQFGAKGHEVPQLIAAALLNHPHDAATVYYRSRPLM
                     LGVGLSPWEAFASNMHKVEGVSGGRDIGVVFNHKQPGGVTVLPASGDVGAQFTPAVGW
                     AQAVRYRVEVMGQQEYDGAVALVHAGDGATSTNGFWSAINIAGPRKLPYVMLIEDNRY
                     ALSVPWRYQSAATSVVENLANFQGLNIQSVEGGDIVELYNALHSAISSARQGGGAQLV
                     HVKVPRLTGHNWQDPAAYKTPEEKEEDLRRDPIARLTAYLQQEHGVPEGKIEEMRQEA
                     EGFARQQAEAAWSQGTEPQVGDGLKHLFAPARTSASTTPITEGPRLTMQQAIKQALAD
                     EMAHDASVVVFGEDVGAFGGVHRVTEGLQAKYGEARCFDTSLNEEGIIGRAVGMSMNG
                     LRPVPEIQFRKYADPAHEQITDAGSLRWRTNGRFGGPMVMRIPVGYQVMGGDPWHAVC
                     GEAVFAHLPGWQIAYPATAADAVGLLRTALRGDNPVVFLEHRLLYRYREANAPYPGPD
                     YEVPFGKARIARQGSDALIVTWGDTVYRSIEAANAVAGATGAEARVLDLRTIVPWDKE
                     AVLAAVRETGRVLIVHEDTITCGFGAENAAQVAEEAFMYLDAPVKRVAMEDVPSPTHH
                     NLFEEVMPTSRKIQKALEELLRF"
     gene            complement(2789..4492)
                     /locus_tag="VFH60_11720"
     CDS             complement(2789..4492)
                     /locus_tag="VFH60_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6314494.1"
                     /translation="MIKETTADPVASTAQKPPSLVDVIKNSNFAKLWGAQVLSQTAQQ
                     IVNFALVLQVASITKSSTATSGIIIAFTVPAILFAAIAGVFVERNSKKLMLVLTNILR
                     GVMVLAFLFTDPRWGVGAVLPIFYTATFLFASVSQFFNPAEAALIPQLVKKRELVSAN
                     ALFNLTLSATQLGGFVVLGPLLLNTIFHNNYNGLYFVCFLLCIAAAGMTYLLPNDAPT
                     ETLAQRRARGEKVNAMSVAAGTGEIARSGFRQALSELVEGWSFIRRDQVIMSAILYWS
                     IAIAVFMMLGTIGPNFLKLVLGVDESKLFYILLPGGIGLVVGVLLVGRLASERNREAM
                     INWNLFFAGITLLLFALVPVTLRWFVSLFGPAAVLPAHVNVASEATAQVGAAMGWASE
                     LFGSGVVQAEYAFPPNWEWVLLALLGGLTLILGLFNSFISVPAQTALQERAPEAIRAR
                     VFSAFYTVSNAILIVPVFFAGAMADSFGYQQTVAVIGVAVIAIAGLGLYRSRQRRSST
                     DPVSEGPITAEEVQAALTAGSPAPRPMPADTQQEHTGDSQPLEPPQPQVPPTSDRDED
                     K"
     gene            complement(4540..4749)
                     /locus_tag="VFH60_11725"
     CDS             complement(4540..4749)
                     /locus_tag="VFH60_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HET6314495.1"
                     /translation="MQPMDIRMGDVYTLRKPHPCGSYDWRVVRVGADIGIRCAKCDHK
                     VLMARSEFERRVKKLLARESGDGGS"
     gene            complement(4884..5381)
                     /locus_tag="VFH60_11730"
     CDS             complement(4884..5381)
                     /locus_tag="VFH60_11730"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6314496.1"
                     /translation="MTGEEVDAGGALQDVWPDATCYGCGPANSEGLRIKSYWSEDGAE
                     VVCRFEPQPRYNAGFPNVMYGGLVASLIDCHSIWTAIAWSYREEGREHGSAPSISYVT
                     GTLNLRFLKPTALDRPVVLRARVTELQPRKALVACSLYSGDEETARGEVTAVRFSMDK
                     ALGAT"
BASE COUNT          981 a         1837 c         1636 g          970 t
ORIGIN      
        1 taaaatctca acaactcttc cagtgccttt tgtatcttcc tgctggtggg catgacttcc
       61 tcgaacaggt tgtggtgggt gggagagggc acgtcctcca tcgccacccg tttcaccggc
      121 gcatcgaggt acataaacgc ctcctcggcc acctgcgccg cattctccgc gccgaagccg
      181 cacgtgatcg tgtcctcgtg cacgataagc acccgcccag tctcccgcac cgccgccagg
      241 accgcttcct tgtcccacgg cactatcgtg cgcaaatcca acacgcgcgc ctccgccccg
      301 gtcgccccgg ccaccgcgtt cgccgcctcg atggagcggt acaccgtgtc gccccaggta
      361 actatcaaag catcgctgcc ctgtctcgcg atacgcgcct tgccgaacgg tacctcgtag
      421 tcggggccgg ggtaaggggc attggcctcg cgatagcggt acagcaggcg gtgctccagg
      481 aacaccaccg ggttatcgcc cctgagcgcc gtcctcagca ggcccaccgc gtccgcggcg
      541 gttgccgggt aggctatctg ccagccgggc aggtgggcga acaccgcctc gccacacacc
      601 gcgtgccagg ggtcgccgcc catcacctgg tagcccaccg ggatgcgcat caccattggc
      661 ccgccaaagc gcccgttggt gcgccagcgc aggctgcccg cgtcggttat ctgctcgtgc
      721 gcggggtctg cgtacttgcg gaactggatt tcgggcaccg gacgcaggcc gttcatcgac
      781 atgcccacgg cccgcccgat tatcccctcc tcgttcaggc tcgtgtcgaa gcagcgcgcc
      841 tcgccgtact tcgcctgcaa cccctccgtc acgcggtgca cgccgccgaa cgcccccacg
      901 tcctccccga acaccaccac gcttgcatcg tgcgccatct cgtcggccag cgcctgcttg
      961 atagcctgct gcatggtgag gcgcggcccc tcggtgatgg gcgttgtgct cgcgctcgtg
     1021 cgggccgggg cgaacaggtg cttgaggccg tcgcccacct gcggctcagt gccctggctc
     1081 catgccgcct ccgcctgctg ccgggcaaag ccttccgcct cctgccgcat ctcctcgatc
     1141 ttcccctcag gcacgccatg ttcctgctgc aagtaggcgg tcaggcgggc gattgggtcg
     1201 cggcgcaggt cttcctcttt ctcctcaggc gtcttgtagg cggcggggtc ctgccagtta
     1261 tgcccggtga ggcgcggcac cttcacgtgc actagctgcg cccctccccc ctgcctggcg
     1321 ctgctgatcg cggagtgcag ggcgttgtaa agctccacga tgtcgccgcc ctccacggac
     1381 tgtatgttga ggccctggaa gttagccagg ttctccacga ccgacgtggc cgccgactgg
     1441 taccgccagg gcaccgagag ggcgtaccgg ttgtcctcta tcagcatgac gtaaggcagc
     1501 ttgcgcggcc cggctatgtt gatggcgctc cagaagccgt tggtggaggt agcgccgtcc
     1561 cccgcgtgca ccagggccac cgcgccgtcg tactcctgct gccccatgac ctccacccgg
     1621 tagcgcaccg cctgcgccca gcccaccgcc ggggtgaact gcgcgcccac gtcgccggag
     1681 gccggcagca cggtcacgcc acccggctgc ttgtggttga acaccacgcc gatgtcacgc
     1741 ccgcccgaca cgccctcgac cttgtgcatg ttgctggcga acgcctccca cggcgagagg
     1801 cccaccccca gcatcagcgg acgcgagcgg tagtacaccg tggcggcgtc gtgcgggtga
     1861 ttcagcaggg ccgccgctat caactgcggc acctcgtgcc ccttcgcccc gaactggaac
     1921 ttgagcttgc cttccagcgg cttatcgggg ttggggcggt actccttggt cacttccagg
     1981 tcgtccatag cccgcgaaat aagggcgcgt tccaggatgg agagccacca accggcggag
     2041 gggtttgcca tgtcggcggg cgcgggcacc ggcagccagt cggctccaat gctcgcggat
     2101 tgcgtgctga gtgccatttc aactcctcat tgagcagaga ttatacgtgc agcgaacctg
     2161 attgcaggct ccctccatca taccagaaat agccccacct tccgagaggg acggacaggg
     2221 acggaaccgc caagacgcca ggagacgaac ggttcaccgc agagacgcag agagcgcaga
     2281 ggttacgcag agagaattca ggctttgggg agcaaagagg accaggggta ctgcattcgt
     2341 accctggtcc tctttgctca gaacgcctac ttaatcttgg cgttcttgac gtcttggcgg
     2401 ttccgtctcg taacctgtag cgacgggctt agaagcccct ggaggtcgtg atgaggacga
     2461 cgcccaagac caggagggcc acctgtatca tgatgaagat gacggacttg agcttggaca
     2521 taatggcgga caacatagcg ggcgactctc ctctaggaag gtagtacagc cagatagcac
     2581 gggagaagcg gcaacacaaa gcgcggacac ccacataagg tacccgaaac gccctgcgag
     2641 ccgcttaccg ataagctccg gctggctgga tgcgagtcta cccggtcgga gtctgccgcc
     2701 gcaagtggat ggggccaaaa tttagcctta gagatggtcc tggttgggga attacgtaat
     2761 agtacgtatc gcggacccgg cccgatattt acttgtcctc gtctctgtct gaggtgggcg
     2821 gtacctgggg ctggggaggc tcaagcggct ggctgtcgcc cgtgtgttcc tgctgggtgt
     2881 cggcgggcat gggcctcggc gcggggcttc cggcggtcag cgcggcctgg acctcttcgg
     2941 ctgtgatggg accttcgctg accgggtcgg tcgagctacg gcgctggcgg ctgcggtaca
     3001 atcccagccc cgctatcgcg atcaccgcca cgcctattac ggccaccgtc tgctggtagc
     3061 cgaaagagtc ggccatcgca ccggcgaaga atacgggcac gatcagtatc gcgttcgaca
     3121 cagtgtagaa ggcgctgaac acgcgggcgc ggatggcttc gggggcgcgc tcctgtagcg
     3181 ccgtctgcgc gggcaccgat atgaagctgt tgaacagccc cagtatcagc gtcaggccgc
     3241 caagcagtgc caacagcacc cattcccagt tcggcgggaa ggcatactcg gcctgcacca
     3301 ctccgctgcc gaacagctca gaggcccagc ccatcgcagc ccccacctgg gcagtggctt
     3361 ccgaggccac gttgacgtga gcgggcagca cggcggccgg cccgaacagg ctgacgaacc
     3421 accggagcgt gacaggcacc agggcgaaca gcagcagcgt aatcccggcg aagaacaggt
     3481 tccagttgat catcgcttcc cggttgcgct cgctcgccag ccgccccacc agcagcacgc
     3541 ccaccaccag gccgatgccg ccgggcagca ggatgtagaa cagcttgctc tcgtccaccc
     3601 ccagcaccag cttcaggaag ttggggccaa tcgtgccgag catcatgaac accgcgatgg
     3661 cgatggacca gtagaggatg gcgctcataa tcacctggtc gcgccggatg aagctccagc
     3721 cttccaccag ctcggacagc gcctgccgga agccgctccg ggcgatctcg cccgtgcccg
     3781 ccgccacaga catagcgttc accttttcgc cccgcgccct ccgctgggcc aatgtctcgg
     3841 tcggcgcgtc gttgggcagc aagtaagtca tgcccgccgc cgctatacac agcaggaagc
     3901 aaacgaagta gaggccgttg tagttgttat ggaagatcgt gttcagcagc agcggtccga
     3961 gcaccacgaa gccgccaagc tgggtagccg acagcgtcag gttgaacagc gcgttggccg
     4021 acacaagctc tcgcttcttg accaactgtg ggattagggc cgcttccgcc gggttgaaga
     4081 actggctcac cgaggcgaac aagaacgtcg ccgtgtagaa gatcggcagc accgcgccca
     4141 ctccccagcg cgggtcggtg aaaagaaagg ccagcaccat cacgccgcgc aagatgttgg
     4201 tcagcaccag catcagcttc ttcgagttgc gctcgacgaa cacgcccgct atagccgcga
     4261 acaggatggc gggcaccgta aaggcgatga tgatgcccga cgtggcggtg ctcgacttgg
     4321 tgatagaggc gacctgcaat accagcgcga aattgactat ctgctgtgcc gtctgcgaca
     4381 gcacctgcgc gccccacagc ttggcaaaat tgctgttctt gattacatcc accagcgaag
     4441 gcggcttttg tgcggttgaa gcgaccgggt cggccgttgt ttctttgatc atagttgtga
     4501 cgtgacctgg caagaaaatt acgaattaag aattaaaaat tacgaaccgc cgtccccgct
     4561 ctcccttgca agcagcttct tcacccggcg ctcgaactcg gaccgggcca tgagcacctt
     4621 gtggtcgcac ttggcgcagc gaatgccgat gtcggccccg accctcacca cgcgccagtc
     4681 gtagctaccg cacgggtggg gcttgcggag ggtgtataca tcccccattc tgatgtccat
     4741 cggctgcatt ttacgtgccc cgctccacgc ccaggcccaa tatctccctg caaaccggca
     4801 catgtctctg ccgctgtgcg cttcgtcatt ataacatgca ggcccgaacc cgtaatgtgt
     4861 aacccgtaaa tcctaaatcc taactacgtg gctcccagcg ccttgtccat gctaaagcgc
     4921 acggccgtga cctcgcccct ggcggtctcc tcgtcgcccg agtagagcga acaggcgacc
     4981 agggccttgc gcggctgcaa ctccgtcacc ctggcgcgca gcaccacggg gcggtcgagg
     5041 gcggtgggct taaggaagcg gaggttgagg gtgcccgtca cgtagctgat gctgggggcc
     5101 gaaccgtgct cccggccctc ctcccggtaa ctccacgcga tggccgtcca gatcgagtgg
     5161 cagtcgataa gggaagccac cagtccgccg tacatgacgt tggggaagcc cgcgttgtat
     5221 ctcggctgcg gctcgaaccg gcacaccacc tccgccccat cctccgacca gtagctcttg
     5281 atgcgcaacc cctcactgtt cgcgggcccg cagccgtagc aagtagcatc cggccacaca
     5341 tcctgcaaag cccctccagc atcgacctct tcgcccgtca tgcttcctct cctggataaa
     5401 gtgtcggcaa caggccatta tagc
//
LOCUS       DASRWZ010000740         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_539375, whole genome shotgun sequence.
ACCESSION   DASRWZ010000740 DASRWZ010000000
VERSION     DASRWZ010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539375"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..>2896
                     /locus_tag="VFH60_11735"
     CDS             426..>2896
                     /locus_tag="VFH60_11735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6314497.1"
                     /translation="MVPPTGVTDEHTNGHAEAPPSPNAAPQADGHAPGDGTATVASLP
                     APIVADVEAPAAVDGAAPDVKPEPVARASRGRTPEPWLWRTKGFWLGGIGLAFAMNAQ
                     KVLIVDREVVGSIRWYVLAIFLVLVAWLGTYRNKSVLAEVVREERVERKSRGRTSLEP
                     ARTAVVPSAPIASATTPATTLPTRPRAAKPTPAPTSTLPVRPTNRAQPPPAPYVAPKT
                     DLRSRGADLLATARDRYPGLVRSGPRYLLAIVALLLNVYSANTLRGDYYSAVGSIGWL
                     ISLVLLLVAFIGERRPKLRDPNAGAADNEDRDRVRMAVVIEIGFVLAILMLAFYLRVH
                     RLGDLFGGMHGDEGETGMEGIKILEGNRVSPFMTGWFAHPNFSFWAVALSMGAFGKTL
                     FGLRILSTILSVLMIVPFYLLVRMWFGVRTALIAGILLAVMDVSIYFGKLGLNNIMTP
                     LFLVGGIYFLARGLRSLRATDFVLAGYSSMLTLYFYFAGRLTPFLLAAMLGYLFLLMP
                     LVGLPGAYLEQRREDRLRSRLLALRSAFAAQWRRVTPYFSRLLIFGVACLCMASPWGV
                     FYLDHQGEMNSRSNDKFVLFPTNEPRMASEYQANHDPLYVGLRMPTSSDIYPFLPVVF
                     EQTPVSVKVAEDGFFARVFWGQTTKTLSMFTYRADNSSFFTLTGEPAAKPIEAALLVL
                     GIAWAVARWRDHRMAILSIWFWGTVFAGGVLTIDAPYMPRMVGLVPVLAIFIALPLNK
                     MAAEFYNAFGRVRAAKPTRRARWQPIVARAVPAVLIVGTLVYLGLQNATDYDRFLNTY
                     PYRDVAGQAYFVREMNKQVAAEGKP"
BASE COUNT          496 a          930 c          889 g          581 t
ORIGIN      
        1 agccagaggg catccatgcg gggcgtcgtg tagggcacac ccacaggcct accgccgtgc
       61 aaccttgccc ctagaccgga agtgcccgcc ctcggctcaa tccggtacgt ttattgacgt
      121 gccacatcag catgccttac aattgctgtt aagtgcaaga ctatctcatc agtgtgagga
      181 cgggcgacca tacctgctac cctgaccaaa gaccctgagc cactggcgag aaaccccaag
      241 cgcggactat gcaccgccca ggctcgccgc ttaaggggag cagcaccctt cgcatatttc
      301 ttgttgccat taccgcgtgc cgcgagctac atgcaaactg atcccaaagg gcactgttta
      361 tgaaaccatg gttgcctgaa gaggcagcta gtaagggtaa acgcgcccgc gaaagcgcac
      421 aacccgtggt ccctcccact ggcgtgaccg acgagcacac gaacgggcac gcggaagcgc
      481 ccccgtcgcc taacgccgcc cctcaggccg atggccacgc tcccggtgac ggtactgcta
      541 ctgttgccag cctccccgca cccattgtcg ctgatgtcga agcaccggcg gctgtcgatg
      601 gtgcggcacc tgacgttaag ccggaacctg tggcacgtgc ctcgcggggg cgaacccccg
      661 aaccctggct gtggcggacc aaggggttct ggcttggggg tattggcctc gccttcgcga
      721 tgaacgcgca gaaggtcctc atcgtggacc gcgaagtcgt cggctcgatt cgttggtacg
      781 tgctcgctat tttcctcgtg ctggtggcat ggctcggcac ctatcgcaac aagagcgtgc
      841 ttgctgaggt ggtgcgggag gagcgagtcg agcgcaaatc gcgggggagg acttccctgg
      901 agcctgcccg cacggctgta gtaccgtccg cgccaatagc atcagccacg acaccggcga
      961 ccacgcttcc aacacgcccc agggccgcta aacccacccc cgcgcccacg agcacgcttc
     1021 ccgtacggcc taccaaccgc gcgcagcctc cgccggcccc ctatgtcgca cccaagacgg
     1081 acctgcgttc gcgcggggct gacctgcttg cgactgctcg tgacaggtac cccggcctgg
     1141 tgaggagtgg gcctcgctac ctgcttgcaa ttgtggccct tctcctgaac gtctacagcg
     1201 ccaacacttt gcgcggcgac tactattccg cggttggaag cattgggtgg ctgattagcc
     1261 tggtgctgct gttggtagcg ttcatcggag agcggcggcc gaagctgcgc gatccgaatg
     1321 cgggtgcagc cgacaacgag gaccgcgata gggtccgcat ggcggttgtg attgagatcg
     1381 gcttcgtgct ggccatactc atgctggcgt tctacttgcg cgtgcatcgc ctgggcgacc
     1441 tgtttggcgg tatgcacgga gacgaaggcg agacgggcat ggagggcatc aagatcctcg
     1501 aaggcaaccg cgtgtcgccc ttcatgaccg gctggttcgc ccaccccaac ttctccttct
     1561 gggctgtggc cctctcgatg ggcgcgttcg gcaagaccct gtttggcctc cgcatcctct
     1621 ccacgatcct gagcgtgctt atgatcgtgc ccttctacct gctggtacgg atgtggttcg
     1681 gcgtgaggac cgcccttatc gcgggcatac tgctggcagt gatggacgtg tcgatctact
     1741 tcggtaagct gggcctcaac aacatcatga cgcccctctt cctcgtgggg ggtatctact
     1801 tcctcgcgag agggctgcgc agcctgcggg ccacggactt tgtgctcgcg ggctacagtt
     1861 cgatgcttac cctctacttc tacttcgcgg gcagactaac accattcctg ctggccgcga
     1921 tgctggggta cctgttcttg ctgatgccgc tagtgggact accgggcgcg tatttggagc
     1981 agcgcaggga ggatagactc cgctccaggc tgctggcctt gcggtctgcc tttgccgcgc
     2041 agtggcggcg cgtgaccccc tatttctcca ggctgctcat ttttggcgtg gcgtgtctct
     2101 gcatggcctc gccctggggc gtgttctacc tggaccacca gggcgaaatg aactcacgct
     2161 ccaatgacaa gttcgtgctg ttcccgacta acgagccgag aatggcgagc gagtaccagg
     2221 ctaaccacga cccgctctac gtcggccttc gtatgccgac ctccagcgac atctatccct
     2281 tcctgccggt ggtgttcgag cagacaccgg taagtgtgaa ggtggcggag gacgggttct
     2341 tcgcccgcgt cttctggggg cagaccacca agacgctgtc gatgttcacc taccgggccg
     2401 acaatagctc cttctttacg cttaccggcg aaccggctgc caaaccgatc gaggcggcct
     2461 tgctggtgct gggcatagcc tgggccgtcg cgcggtggcg cgaccaccgc atggcgatac
     2521 tctctatctg gttctggggg accgtgttcg ccgggggcgt gctgacgatt gacgcgcctt
     2581 atatgccccg catggtgggc ctggtgcccg tgctggctat cttcatcgcc ctcccactga
     2641 acaagatggc tgccgagttc tacaacgcct tcgggagggt gcgagcggcc aagccaacac
     2701 ggcgggcgcg ctggcagcct atcgtggcgc gcgccgtgcc tgccgtgttg attgtgggca
     2761 cgctggttta cctagggctg caaaatgcca ccgactacga ccgcttcctg aacacatatc
     2821 cctaccggga cgtggcgggg caggcctatt tcgtccgcga gatgaacaag caggtagccg
     2881 cggaaggcaa gccgat
//
LOCUS       DASRWZ010000741         4654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1156270, whole genome shotgun sequence.
ACCESSION   DASRWZ010000741 DASRWZ010000000
VERSION     DASRWZ010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156270"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..1516)
                     /locus_tag="VFH60_11740"
                     /pseudo
     CDS             complement(383..1516)
                     /locus_tag="VFH60_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            complement(1527..2498)
                     /locus_tag="VFH60_11745"
     CDS             complement(1527..2498)
                     /locus_tag="VFH60_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6314498.1"
                     /translation="MAEVRVGFIGAGGIAGRHVGVLQQFEDVKIVAFADPSEERAGGL
                     AGWAGAAAYTDYRQMLDREKLDALYICVPPFAHGEPELAAAERDLPFFVEKPLATDRE
                     TAERIAASVEQKGLITAVGYHWRYLTTIDQAQELLESNPARLALGYWLDQTPPPYWWQ
                     KEELSGGQMVEQTTHIFDIARVLVGEIEEVYGVGSRKDREGFPDLDVCDVSAATLKFS
                     TGAVGTMSSTCLLNWPHRVGLHLFSEGMAIELSDRDIMIDVGQGRPVHYQQGDPVVRE
                     DRDFIDAVQGKANRVRCPYSDALKTHYVTTAAARSLVEGRPLRPFER"
     gene            complement(2525..3676)
                     /locus_tag="VFH60_11750"
     CDS             complement(2525..3676)
                     /locus_tag="VFH60_11750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6314499.1"
                     /translation="MKPRLCLFTDSIEPSGVGEHMLCLAEELRSQYDVFFLGWSSTGL
                     YARAAERGFPVYELSVDWYVNKDLLIQLEPQVMHVHAGASWEGHGGVWAARNASVPHV
                     LRTEHLPYLITEKWQQDQYAGFLDWVDAIICVSEGVRRTYAEAGVPEDKLRVVLNGIP
                     ITPERHDSNTAACRVRTELGLDPDSRLVLTVGRLTEQKGYTYLLDAACIVAAELPGTH
                     FLLAGEGPLERQLRARAGACGASRNVHFLGPRPGVAHLMSSCDLFVLPSLFEGLPLVV
                     LEAMSAGRAVVATRVCGTEEAVAEAVTGRLVPARDPDALAAAITYALLNHDVRDAWGV
                     AGRERVSVQFTANRMAGEVAAIYDTLLAPSRAAVHPVESLVTPVQAHNY"
     gene            complement(3744..4406)
                     /locus_tag="VFH60_11755"
     CDS             complement(3744..4406)
                     /locus_tag="VFH60_11755"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6314500.1"
                     /translation="MKIKGVILDVDGTLVDSNDAHIRSWLEALSQHGYNCTYEEIRRL
                     IGMGSDNLLPAAIGVEKDTSEGKRLSDTWAEVFERDYLPHIKAFPHTRDLLLALKARG
                     LKLVVASSAQEDMLDKLLDIAEAKDLLEESTSADDAENSKPDPDIVEASLRKIGLRPD
                     EVLMLGDTPYDIQAASKLHIGTIGLRCGGWDDEGLKGAVAVYDDPADLLARLDDSPLT
                     RS"
BASE COUNT          804 a         1437 c         1548 g          865 t
ORIGIN      
        1 gtcgtcgtca actacgagac cgcgcatgtt tggtcactgc ctggtgggta gggattcacg
       61 atgattatag cacaccgggc aggaagagcc tctaaaatgg agccgaagtc taatcgaacc
      121 ttaatcttca ccacgccagg aaaagaggaa gttaagttgg gcaaagttgc agtaagcaaa
      181 agaaaaaccc cgaacgaact ttgcagtccc tccggagttt ctcttttgtc ggagacgctc
      241 aacacatctc caacgcccac agtatagggg atgtcaggag gggtgtcaac ggggcagcct
      301 ggcacttttc cacatccacc aagcggtgga agggaaagtt tcagccagca tgttcaatat
      361 tgtagctggg ccgcctgctg tgctcctcta tgacctcctt gaagagtgct tcccacctgg
      421 ggacgagcgc gcagctgctg tattcgcgtt ccactttctc gcgcagtgcg tggccgagct
      481 tgcgcgcaag ggcagggtcc tgtagcagcc gggtgatggc tgttgctacc tcttcgggcg
      541 actcgtgcgg cacgaacaga cccgtcacgg cgtgggtgat ttgttgctcc gagccgttgt
      601 cgcgggtggc tacgacgggc aggcacgcgg cccctgcctc cgatatgacg tggggcatgc
      661 cctcgcctct ggagagccag gcgaacacgt ccaggcccga gagcaggcgc ggtacgtcgt
      721 cgcggtcgcc caggaacgac accgcgtcac caagccccag gtctgaggca agggcgacaa
      781 gctcggcggc gtactccggc atgaacgcat cgggtccgcc tatgattacg aagcgggcag
      841 agtgcaccgt acgccggacg atggccgctg ctcggatgaa gtcctccacc cgcttcttgc
      901 ggtccagcct cccggcccag cctacgaggg gcgcgccctc cggtgccttc agctcctcgc
      961 ggaccgaggc ccggcgcgcg gggtcgaatt cctcaaggtc caccatcgag ggtagttcta
     1021 tagcgtagcg ttcgcggccg ggcatgctgc gggcggcggc atccctgatg gacctgcaca
     1081 cgcctacgta gcggctcgtg aggtgcttgg ggcctgagag ggcttcgctc accaggccgc
     1141 cgtgctctat cagcgggggc cggagttgtg ggtcgatgcg ctccaacgcg ggatacacgt
     1201 ctggcacgcc ctggcaagcc accacgaggt cgtagcgggg cagctttgtg gcgagatagg
     1261 cgacagtttc atcgaaggag aggctgtagg gcgtttcgtc tatcgcaacg ccgagcgccc
     1321 gcaattgctc gcgggcctgc gcgggcatgt tgggcttgta gaagcaggcc actacctcga
     1381 tgtcgaactt gcggcggtcc atgtgtttgg cgagcaggcg cacctccgtc tcctcaccgc
     1441 cgaccacgag ccagccgaac acgaacagca ccctgtacgg gcgctcgcct ccattgcgcg
     1501 agagcgttct ctccatgcct gcccccttat cgctcgaacg gcctgagggg ccttccctcc
     1561 acgagggagc gggcggcggc tgtggtcacg tagtgggtct tgagggcatc ggagtagggg
     1621 cagcgcaccc tgttggcctt gccctgcacc gcgtcgatga agtcgcggtc ctcgcgcacc
     1681 acggggtcgc cttgctggta atgcacgggc cgcccctgcc ccacgtctat catgatgtcg
     1741 cggtcgctaa gctcgatggc catgccctcc gagaagaggt gtaggccgac gcggtgaggc
     1801 cagttgagca agcaggtgga ggacatggtg cccacggccc cggtgctgaa cttgagggta
     1861 gccgccgaca cgtcgcacac gtcgaggtcg gggaagccct cgcggtcctt gcgggagcct
     1921 acgccgtaca cttcctctat ttcgcccacc agcactctcg cgatgtcgaa aatatgggtg
     1981 gtctgttcca ccatctgccc gccggaaagc tcctctttct gccaccaata gggcggcggg
     2041 gtctggtcga gccagtagcc gagggccagg cgcgcggggt tcgactccag aagctcctgg
     2101 gcctggtcga tggtggtcag gtagcgccag tggtagccta cggcggttat gaggcccttc
     2161 tgctccacgg aggcagctat tcgttcggcg gtctcgcggt cggtcgccag gggcttctcg
     2221 acgaagaagg gcaggtcgcg ttcggcggcg gcaagctccg gctcgccgtg ggcgaatggc
     2281 ggcacgcaga tatagagggc gtccagcttt tcgcggtcga gcatctgccg gtagtcggta
     2341 tatgcggcag cgccggccca gcccgccagt ccgccggcgc gctcttccga cgggtccgca
     2401 aaggcgacga ttttgacgtc ctcgaactgt tggagcacgc ccacgtgacg gcccgcgatg
     2461 cccccagcac cgataaatcc tacgcgtacc tctgccattg tgtaactcct ctgaaggctc
     2521 ccggttagta attgtgagct tgtacgggcg taacaagtga ctcgacggga tggacggccg
     2581 cgcgcgaggg ggcgagcagg gtgtcgtaaa tggccgccac ttcgcccgcc atgcggttcg
     2641 cggtaaattg cacgcttacc cgctcccggc ccgccacgcc ccaggcgtcg cgcacgtcgt
     2701 ggttgagcag cgcgtaggtt atggctgcgg ccagggcgtc agggtcgcgg gcgggtacga
     2761 gccgcccggt gactgcctca gcgacggctt cctcggtgcc acacacgcgg gtagcgacga
     2821 ctgcccgccc ggcagacata gcttccagca ccaccagggg cagtccctcg aagagggagg
     2881 ggagcacaaa gaggtcgcag gacgacatga ggtgggccac gccgggccgg gggccgagga
     2941 aatggacgtt gcgagaggcc ccgcatgcac cggccctcgc ccttagctgg cgctcaagcg
     3001 gaccctctcc cgcgagcagg aagtgagtgc cgggaagctc agccgctact atgcaggccg
     3061 catccagcag gtaagtgtaa cctttttgct ccgtcaggcg gccaaccgtc agcaccaacc
     3121 ggctgtcggg gtccagaccg agttcggtgc ggacccggca ggcggctgtg ttgctgtcat
     3181 gccgctccgg ggtgatgggt atgccgttca ggaccacccg gagtttgtcc tcggggacac
     3241 cggcctcggc gtaggtccgg cgcacacctt cggagacgca gattatggcg tccacccagt
     3301 cgaggaagcc cgcgtactgg tcctgctgcc acttctcggt gatgagatag ggtaggtgct
     3361 cggtgcgcag aacgtgtggc actgaggcgt tgcgggccgc ccatacgccc ccatgcccct
     3421 cccacgacgc gcccgcgtgg acgtgcatga cttgaggctc tagctgtatg aggaggtcct
     3481 tgttgacata ccagtccacc gacagctcgt agaccgggaa gccacgctcg gcggcgcgcg
     3541 cgtacaggcc ggtggaggac cagccgagga agaagacgtc gtattgagac ctgagttctt
     3601 ccgcgaggca aagcatgtgc tcgcccacgc ctgaaggctc gatgctgtct gtaaacaggc
     3661 acaatcgtgg tttcactatg tactccggta ggggtaggtt tccagcaggt ggccgcggtc
     3721 tgtggtgcga ccggctcgcg ctgtcaggaa cgtgttagtg gcgaatcgtc caggcgtgcc
     3781 agcaggtcgg ccgggtcgtc gtacacggcc acggcacctt tgaggccctc atcgtcccag
     3841 ccgccgcaac gcaggccgat ggtgcctatg tgcagcttgc ttgcggcctg gatgtcgtag
     3901 ggagtgtcgc ccagcatcag gacctcgtcg gggcggaggc cgatcttccg aagtgaggcc
     3961 tctacgatgt cgggatcggg cttggagttc tcggcgtcgt cggccgaggt gctttcttca
     4021 aggaggtcct tggcctcggc gatgtcgagc agtttgtcca gcatgtcctc ctgcgccgag
     4081 cttgccacga ccagcttgag gccgcgcgct ttgagggcga gcagcaagtc cctcgtgtgg
     4141 ggaaaggctt tgatgtgggg gaggtagtcg cgctcgaaca cctcggccca ggtgtcgctg
     4201 aggcgcttgc cttcggacgt gtctttctcg acgccgatgg ctgcggggag caggttgtcg
     4261 ctgcccatgc cgatgaggcg gcggatttct tcgtaagtgc agttatagcc gtgttgcgac
     4321 agcgcctcca accaggagcg tatgtgcgcg tcgttgctgt cgacgagcgt gccgtcaacg
     4381 tcgaggatta cacctttgat tttcaagttc gttctccagg tctgggcttg tattaccaac
     4441 cctatttaag aaactaagaa tagaagggaa agggagaact gggggacact cccagccccc
     4501 cgtcagggga gaacccctgc acccccttcc gagggagaac cgggggacac ccccagcctc
     4561 ggtcagggga gaacccctgc accccttcat ttcctccacc gtttacgggg gcacgcgggg
     4621 gccgactgca caattggcag cggccccgct agca
//
LOCUS       DASRWZ010000742         6263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_882602, whole genome shotgun sequence.
ACCESSION   DASRWZ010000742 DASRWZ010000000
VERSION     DASRWZ010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882602"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFH60_11760"
     CDS             complement(<1..298)
                     /locus_tag="VFH60_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314501.1"
                     /translation="MAVATGTAVLVRVLVATAVLVLVRVAVPVATAVLVLVLVAAVVA
                     VDDGTTTEVAVPVAVVVEVIGCVAVAVVVEVIACVVAVVVAVAGCVVAVAVAVAT"
     gene            285..974
                     /locus_tag="VFH60_11765"
     CDS             285..974
                     /locus_tag="VFH60_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314502.1"
                     /translation="MATATACAIQFADVQPGDTFYEFIRCLACRQIVGGYPCGGPGEA
                     CNQDNEPYYRPGANVTRGQLSKIIANSAGLDDTPAEGQQQFADVPPGSPFYEFVERLA
                     QTGAIGGYPCGGPGVSEPCDSEGRPYFRPNNPATRGQISKIVSIAAGFEEDVPEDQQT
                     FTDVPTDSPFWVYIERLAGRGIISGYGEEERCPETGAPCFRYNDNTTRGQMAKIAANA
                     FFPNCQTPARP"
     gene            1114..2613
                     /locus_tag="VFH60_11770"
     CDS             1114..2613
                     /locus_tag="VFH60_11770"
                     /inference="COORDINATES: protein motif:HMM:NF037273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314503.1"
                     /translation="MPSQDTGLTTPSLGYLNSLRGAMSQPLGSWEGFYRAQSPSMNFA
                     LRYQLAFASYALALMSQRTPAYRAPYAEALRGAIKKMLHVATWGYWRAPVGVGAQGGV
                     ASQGHIAVLAGPHQRGPLGPPSDPIARDNLQYSGHLSTMLGLYEKVSGDRRYDEPFAL
                     EDDESGARYTYTHGEVARRIYTQMRENGFGGVCCEKGMAYVPCNNYSMASNALHDLLH
                     GTDYSLANGRWLKTVRDKMVLKGPALRGVFGTSYMQDFRLATPVAFNFTDVWGLAFLL
                     PFDRALVRKLYGRFKKKAIHRDGEGAYVESSGLSERMEISDVPINTGFGLILARGVGD
                     LKLADALSAYAHAAFEAGWEGTRYLLSGAPRTLHATALFALAECMQPGGEDFTSLFNA
                     PASTSTGDPALLQLEDASGRVGVFEAEYSREVRVLRIGLRQVGEPGVLRGAGAVAVAL
                     HVGNLPGLAQVQVTGAPQEEHGWIGEGSLRLNVTVGPQEDVRCTISLTQ"
     gene            2830..3624
                     /locus_tag="VFH60_11775"
     CDS             2830..3624
                     /locus_tag="VFH60_11775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6314504.1"
                     /translation="MEEPQTWHYGVVARWWAEFNTDGPEIAYFQSVIERHGQPALDVA
                     CGTGRLLVPYLRAGLDVDGCDLSPDMLALCRDKAARDGLVPRLYQQAMHELDLPRAYK
                     TIFMCGAFGLGGDRALAQKALQRAYDHLEPGGVLAFDHYLPYGDARQWQYWLPENRGQ
                     LPRDWRPPGERRLASDGTEYELRSRLVALDPLEQLITMQMRAEQWRDGVLVAQEEYTL
                     KETLYFKSEILLMLERAGFSDVAVYGAYTGREATPEHTVLAFVAKK"
     gene            complement(3762..4844)
                     /locus_tag="VFH60_11780"
     CDS             complement(3762..4844)
                     /locus_tag="VFH60_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET6314505.1"
                     /translation="MSTFKRVSNRIKWTIALIAVAAIVLPSAVAMPASTASAAQARYF
                     PNTGNSVGGAFLKFFDAYGGVRVFGLPLTGELNENGRTVQYFERQRFEYFPEKTGTSY
                     EVQLGLLGTRAAAGKPGAGRVAAFASRKDMVYVSETGHSLSGAFLSFWRANGGVRVLG
                     YPITEPIQEAGLTVQYFERARMEYPPEKAKQGFVVELSLLGRDYLWGGGGGVSPQSFG
                     QAQAAPASSGHTLDGLEQELLNRINGARAGGGLHPVSVDGQVSSLSRHRSNDMVAKGY
                     FSHNAPGGDSYMALLKGAGVPFKLAGEIIAWNNFSNSAEKAFEGFMNSPAHRAIIMDG
                     RYNFAGVGQARSGDGKYYYTVIFVQK"
     gene            5211..6167
                     /locus_tag="VFH60_11785"
     CDS             5211..6167
                     /locus_tag="VFH60_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET6314506.1"
                     /translation="MDKHPLDSWVMVICNPVSGSGTGPAVLTALRRLLDREGVRYACE
                     FSTRRGDPARLARSAVLMGCSAIVAIGGDGTFFEVVNGVMDPEAPTSENGGSPDLAVG
                     LLLAGRGSDFGRSAGIPSDVESACRRILEGRTQLADLGHVTYTAMQGGERSRFFANAA
                     GLGFDGEVTVRANSAPRVMGGGTVPYLTSLLATLGTYRNKQVSVRVDEEDEWHARVNS
                     VIVANGQFFGGGMKIAPGASLSDGLFDIVVLGDLGKLDLVRNVPRVYVGTHITHPKVR
                     VLQGRQVTVDSSDRMLLQADGEVLGKAPAAFRIVPGALRLIV"
BASE COUNT         1176 a         1853 c         2067 g         1167 t
ORIGIN      
        1 gtgttgccac cgccacggct acggccacca cacaacctgc aaccgccaca accacagcca
       61 ccacacaagc gataacctct acaactacgg ctacggccac acaaccaatt acctctacaa
      121 ccacagcgac cgggaccgcc acttctgttg tcgtgccgtc gtccaccgct actaccgcag
      181 ctaccagcac caggacgagc accgcagtag ccacaggaac ggctactcgc accaggacga
      241 gcaccgcagt agccaccagc acccgcacga gcacggcggt acctgtggcc acagccacag
      301 catgcgcgat ccagttcgct gacgtgcagc ctggtgacac cttctacgag ttcatcaggt
      361 gcttagcatg ccgccagata gtaggagggt acccgtgtgg aggacctggt gaggcgtgta
      421 accaggataa cgagccttac tacagaccag gtgctaacgt cacaagaggt cagctgtcca
      481 agatcatagc taactcagca ggtctggacg acactcctgc agagggacag cagcagttcg
      541 cggacgtacc tcctggttct cctttctacg agttcgtgga gcgcctggca caaacaggag
      601 caataggggg atacccgtgc ggaggacctg gagtgagtga gccttgcgac agcgagggta
      661 ggccatactt ccgccccaac aatcctgcaa cgcgtgggca gatctccaag atcgtgtcga
      721 tagctgcagg gttcgaggag gacgtaccag aggaccagca gacgttcaca gacgtaccca
      781 cagacagccc gttctgggtg tacatagagc ggctggcggg cagaggcatc atctcagggt
      841 atggagaaga ggagagatgt cctgagacag gagctccgtg cttccggtac aacgacaaca
      901 ctacgagagg tcagatggcc aagattgctg ctaacgcttt cttccccaac tgccaaacac
      961 cagccaggcc ttaacggcgc tagccaagct acagccttga tataggcaac cagcagagag
     1021 cagagggcct cccttcgcaa gaggggaggc cctctgttat tggtcgggac cgtttgttgt
     1081 atagtgtggg taagcagccc gcaggtgagt aatatgccgt ctcaagatac aggtttaact
     1141 acgccgagcc tgggctacct caactccctg cgcggggcca tgtcccagcc gctcggctcg
     1201 tgggagggct tctacagggc gcaaagcccc tcgatgaact tcgccctgcg ctaccagctt
     1261 gccttcgcct cctatgccct cgccctgatg tcgcagcgca cgcccgctta ccgcgcgcct
     1321 tacgcggaag cgttgcgggg tgctataaag aagatgctgc acgtggcgac ctggggctac
     1381 tggcgggcac ctgtgggtgt gggcgctcag ggaggggtgg cgagccaggg gcatatcgcc
     1441 gtgctggccg gccctcacca acgggggccg ctcggcccgc cttccgaccc catcgcgcgg
     1501 gacaatctac agtactccgg ccacctgagc acgatgctgg ggctgtacga gaaggtatcg
     1561 ggcgaccgcc ggtacgacga gccgttcgcg ctggaggacg atgagagcgg cgcgcgctac
     1621 acctataccc acggggaggt ggcgaggcgt atctacaccc agatgcgaga gaacggcttc
     1681 ggcggggtat gctgcgagaa gggcatggct tacgtgcctt gcaacaacta ttcgatggcc
     1741 tccaatgccc tgcacgacct gctgcacggc accgactaca gcctcgccaa cggcaggtgg
     1801 ctcaagacgg tgcgggacaa gatggtgctc aaaggcccgg cgctgcgcgg ggtgttcggc
     1861 accagctaca tgcaggactt ccgcttagcc acgccggtgg ccttcaactt caccgacgta
     1921 tggggcctgg ccttcctgct gccgttcgat cgggcgctgg tgcgcaagct gtatggcagg
     1981 ttcaagaaga aggcaataca ccgcgacggg gagggggctt acgtcgagtc gtcgggcctc
     2041 agcgagcgca tggagatcag cgacgtgccc ataaacaccg gcttcggcct catcctcgcc
     2101 aggggagtgg gcgacctgaa gctggcggac gcgctgtcag cctacgcgca cgctgcgttc
     2161 gaggcggggt gggaggggac gcgctacctg ctgagtggag cacctcgcac gctgcatgcg
     2221 accgcgctat tcgccctggc cgagtgcatg cagccgggcg gggaggactt caccagcctc
     2281 ttcaacgccc ctgcctccac ttcgacgggt gaccctgctc tgctgcaact ggaagacgcc
     2341 tccgggcggg tgggcgtatt cgaggccgag tactcgcgcg aggtgcgcgt gctgcgtatc
     2401 ggcttgaggc aagtaggcga gcctggggtt ttgcgcgggg cgggggctgt ggctgtcgcg
     2461 ctgcacgtcg gcaatttgcc ggggctggcg caggtgcagg tgaccggcgc tccccaggag
     2521 gagcatggtt ggatagggga gggtagcttg aggctgaatg tgactgttgg gccgcaagag
     2581 gacgtgcgct gtaccatcag cctgacgcag taggggtgcc acgccccaga gtcagcacta
     2641 tgctcccctt tgtcatcctg agcgtagcga aggatcagcc gcccatgttt ggtcggtggc
     2701 actgaggtaa cagtgttcct cttaccacta acatcctgta cgttgaagac tctggcctac
     2761 ccacctgatc cttcgctacg ctcaggatga caagggggag tgtggttgag atgaggaggg
     2821 agtactgtga tggaagaacc gcaaacctgg cattacggcg ttgtggctcg ctggtgggct
     2881 gagttcaaca ctgacgggcc ggagatcgcc tactttcaga gtgtcatcga gcggcacggc
     2941 caacctgccc tcgacgtggc ttgcggcacc gggcgcttgc tggtgcccta cttgcgggcc
     3001 ggcctggacg tggatggctg cgacctctca cccgatatgc tcgccctctg tcgggataaa
     3061 gccgcccgtg acgggcttgt acccaggctg taccagcagg ccatgcacga gttagacctg
     3121 cctcgcgcgt acaagacaat cttcatgtgc ggggcgtttg gcctcggcgg cgaccgcgcg
     3181 ctggctcaaa aagcgcttca acgtgcgtac gaccacctgg agccgggtgg cgtgctggcg
     3241 ttcgaccatt atctcccgta tggggatgcc aggcaatggc aatactggct accggaaaat
     3301 cgcggccagc taccgaggga ctggagaccg cctggcgagc gcagacttgc gtctgatggg
     3361 accgagtacg agttgcgttc caggctggtg gccctggacc cactggaaca acttatcacc
     3421 atgcaaatgc gcgccgaaca gtggcgcgat ggcgtgctgg tcgcgcaaga ggagtacacc
     3481 ctgaaagaaa cgctgtactt caagagtgag attcttttga tgttggagcg cgctggcttc
     3541 agcgatgtgg ccgtgtacgg ggcctatacc ggccgggagg caacgcctga gcacaccgtg
     3601 ctggcgttcg tcgcgaagaa gtgagattgg ggcagggtgc cgtgcagccc atatcgccct
     3661 gtggctgaag cccgccaaat cgttggcagg gcgtttaggt tgcggcgaat aacaaaaggc
     3721 ggtagcgcat ttgctaccgc ctgtcttatt cattgggcgg gctatttctg gacgaatatg
     3781 actgtgtaat aatatttgcc gtcgccgcta cgggcctggc cgacgcccgc gaagttgtac
     3841 ctgccgtcca tgatgatggc tcgatgggcg ggcgagttca tgaagccctc gaaggctttc
     3901 tccgccgagt tgctgaagtt gttccaggcg atgatttcgc ccgccagttt gaacggcacg
     3961 cccgcgccct tgagcagggc catatagctg tcgccacccg gcgcgttgtg ggagaagtag
     4021 cccttggcga ccatgtcgtt ggagcgatgc cgagacaggg acgacacttg cccgtcgacg
     4081 ctcaccgggt gcaacccccc gcccgccctc gcgccgttga tgcggttcag cagttcctgc
     4141 tccagcccat cgagggtgtg gccgctgcta gcgggcgcgg cctgggcctg cccgaacgac
     4201 tgcggcgaga ccccgccacc gccgccccat aggtagtccc taccgagcag gcttagctcc
     4261 accacgaagc cctgtttggc cttctccggg gggtactcca tgcgggcgcg ctcgaagtac
     4321 tgcacggtga ggcccgcctc ctggatgggt tcggtgatag ggtagccgag cacccgcacg
     4381 ccgccgttgg cccgccagaa gctgaggaaa gcgccgctga gagagtggcc ggtctcgctg
     4441 acgtatacca tgtccttgcg ggaggcgaag gcagcgacgc ggcccgcgcc cggcttgccc
     4501 gcggccgccc tggtgcccaa aagcccaagc tgtacctcgt aggaagtgcc cgtcttctcc
     4561 gggaagtact cgaaacgctg gcgctcgaag tactgcaccg tcctgccgtt ctcgttgagt
     4621 tcgcccgtaa gggggaggcc gaacacgcgc actccgccgt aagcgtcgaa gaatttcagg
     4681 aacgctccac ccacgctgtt gcccgtgtta gggaagtaac gtgcttgcgc tgcggacgcg
     4741 gtggaagccg gcattgccac cgccgagggc agcacgatag ccgccactgc tataagggcg
     4801 atggtccact tgatacgatt ggaaactctt ttgaaggtag acaaggtatt attctccgca
     4861 ggtattatgg ccgcaataat caaccgcctg acattacagg cgaacttgcg gtctcaccct
     4921 tgaggcccgc tgaattgtaa ccgaagagaa tagattatag ccataggaaa ttggtcctgg
     4981 acgtacgtca tttgcagtaa tttttccgca gatggacaac gtatcttgat ggttagtcct
     5041 ttgcgcatgc ctattttggc ctactgccgc tttgagccct cgcgagaggc tgctattgcg
     5101 gccggggcgt gcggtggtat aatgaccaac tgtggcgcgt cagggtggtg gataactcgt
     5161 gcaaaagcgt gcaaaaaagt ggatatttcg gggaaaagtg aggtcgcata gtggataaac
     5221 acccactcga tagctgggta atggtgattt gcaacccggt atcaggctcg ggcacggggc
     5281 ctgcggtgct gacggcgttg cggcggctgc ttgacaggga gggcgtgcgc tatgcgtgcg
     5341 agttcagcac gcggcgtggc gaccccgcgc ggcttgctcg ctcggcggtg ctcatggggt
     5401 gcagcgccat cgtggctatc ggcggcgacg gcaccttctt cgaggtggtc aacggggtga
     5461 tggaccccga agccccgacc tcggagaatg gcggcagccc ggacctggcg gtgggcctgt
     5521 tgctggcggg gcggggcagc gatttcgggc ggtcggcggg catcccctcc gacgtggagt
     5581 cagcctgccg ccggattctg gagggcagga cgcagcttgc cgacctgggg cacgtgactt
     5641 ataccgcgat gcagggcggg gagcgctcgc gcttcttcgc caacgccgcc gggctgggct
     5701 tcgatggcga ggtgacggtg cgggcgaact cggctccccg cgtgatgggc ggcggcaccg
     5761 tgccctacct gaccagcctg ttggccaccc tcggcaccta ccgcaacaaa caggtgagcg
     5821 tgcgggtgga cgaggaggac gagtggcacg cccgcgtcaa cagcgtgata gtggcgaatg
     5881 ggcagttctt cggcgggggc atgaagatcg cgccgggggc cagcctctcc gacggcctgt
     5941 tcgatatcgt ggtgctgggc gacctgggca agctcgacct ggtgcgcaac gtgccccgcg
     6001 tgtacgtcgg cacccacata acccacccga aggtgcgcgt gttgcaaggg cggcaggtca
     6061 cggtcgattc ttccgacagg atgttgttgc aggccgacgg cgaggtgctg ggcaaggctc
     6121 ccgcggcttt tcggatcgtg ccgggggcgt tgaggttgat tgtatagtgg ttgaagaggc
     6181 ggtttggtgt aggtagtgcg tggatgctgc taggttggga ggcctggtcc ctctcccttt
     6241 ttgtcatcct gagcgtagcg aag
//
LOCUS       DASRWZ010000743         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1293748, whole genome shotgun sequence.
ACCESSION   DASRWZ010000743 DASRWZ010000000
VERSION     DASRWZ010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293748"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2271
                     /locus_tag="VFH60_11790"
     CDS             <1..2271
                     /locus_tag="VFH60_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HET6314507.1"
                     /translation="ISGLNATFANGMARPGSVGFISQSGALLTAVLDWSTQENVGFST
                     FVSLGSMLDVGWGDMIYYLGDDPHTRSIVIYMETVGDARSFLSAAREVALTKPIIVIK
                     PGRTQAAAKAAASHTGSLTGSDEVLAAGFARAGVLRVSTIAELFYMAEVLAKQPLPKG
                     PRLTIVTNAGGPGVLATDALISEGGELADLSDETMQELDKILPAAWSHNNPVDILGDA
                     SPERYAASLAIAAKDPNSDGLLVVLTPQAMTDPTATAEQLMPYAKIEGKPVLASWMGG
                     ADVEPGARLLNRADVPTFSYPDTAVRMFNYMWRYNYNLRALYETPALPADTESATNRE
                     QAAQIIDTARSEGRTLLTEVESKQLLAAYGVPIVETRIARTEEEAVRWADEIGYPIVV
                     KLYSNTITHKTDVGGVQLNLVDSEAVRQAYRLIEQSVREKAGAEHFQGVTVQEMIQWT
                     GYEVIIGSSIDPQFGPVLLFGMGGQLVEVFKDRALGLPPLTTTLARRMMERTHIYTAL
                     KGVRGRKPVDLEEVEHLLVRFSQLVVEQRWIKERDINPLLASPERLVALDARVVLHEP
                     GVSEEELPRPAIRPYPLQYVREWTTKDGVPLLIRPIRPEDEPLVAEFHKRLSTESVYM
                     RYFQHVQLGDRVAHERLVRICFNDFDREAALVAEHLDPQTGQRSIVGVGRLSRSHVAN
                     EASFAITINDAWQGRGLGQELLRRLVQIGRDEKLDRITAEILPENRGMIRVSEKVGFK
                     IVNDHKGDEVKAVLEL"
     gene            complement(2530..>3196)
                     /locus_tag="VFH60_11795"
     CDS             complement(2530..>3196)
                     /locus_tag="VFH60_11795"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET6314508.1"
                     /translation="AVRDGLTLWDFSKSRLDFVADTELEKKESRFNDGAVDPGGRFWA
                     GTMTFDGSSNGSSLYRLDPDHSLHTMATGFTISNGIGWAPDRKTMYHTDTEKQVIYAY
                     DYDLATGNIANRRDFVRTSGEEGFPDGLAVDSEGFVWSAFYGGWRVTRYDPDGKKERD
                     VYLPVSNVTACTFGGPELTDLYITTAWSGLSVTQRRKQPLAGDLFMLRTGIKGQPESK
                     FRG"
BASE COUNT          593 a         1006 c         1078 g          519 t
ORIGIN      
        1 atcagcggcc tcaacgccac cttcgccaac ggcatggcgc ggcccggcag cgtcggcttc
       61 atcagccaga gcggggcctt gctcaccgcc gtgctcgatt ggagcaccca ggagaacgtg
      121 ggcttcagca ccttcgtgtc gctaggctcg atgctcgacg tgggctgggg cgacatgatc
      181 tactacctgg gcgacgaccc ccacacccgc agcatcgtca tctacatgga gacggtaggc
      241 gatgcccgtt ccttcctctc cgcggcccgc gaggtggccc tcaccaagcc gatcatcgtc
      301 atcaagccgg ggcgcacgca ggcagcggcc aaggcggcgg cctcccacac cggctccctc
      361 acgggcagcg acgaggtgct ggcggcgggg ttcgccaggg cgggcgtgct gcgcgtgagc
      421 acgatagccg agctgttcta catggccgag gtgctggcga agcagcctct gccgaagggg
      481 ccgcgcctca ctatcgtcac caacgcgggc gggccgggcg tgctggctac cgacgccctc
      541 atctcggagg gtggagagct tgccgacctg tcggacgaga cgatgcagga gctggacaag
      601 atactacccg ccgcgtggag ccacaacaac ccggtggaca ttctggggga cgcctcaccc
      661 gaacgctatg cggcgtcgct ggcaatcgcg gccaaggacc ccaacagcga cggcttgctg
      721 gtcgtgctca ccccgcaggc gatgaccgac cccaccgcta ccgccgagca gctaatgccc
      781 tacgcgaaga tagagggcaa gccggtgctg gctagctgga tgggcggcgc ggacgtggag
      841 ccgggcgcac ggctgctcaa cagggccgac gtgccgacct tctcgtaccc cgacaccgcc
      901 gtgcgcatgt tcaactacat gtggcgctac aactacaacc tgcgcgccct ctacgagacc
      961 cccgccctgc ccgccgacac cgagagcgcc accaaccgcg agcaggcggc ccaaatcatc
     1021 gataccgcac gctcggaggg gcgcaccctg ctgaccgagg tggagtcgaa gcagctactg
     1081 gcggcgtacg gcgtgcccat cgtggagacg cgcatcgccc gcaccgagga agaggccgtg
     1141 cgctgggccg acgagatcgg ctaccccatc gtggtcaagc tctactccaa caccatcacg
     1201 cacaagaccg acgtgggcgg cgtgcaactc aacctggtgg atagcgaggc agtgaggcag
     1261 gcttacaggt tgatagagca gtcggtgaga gagaaggcgg gcgcggagca tttccagggc
     1321 gtcaccgtgc aggagatgat ccagtggacc ggctacgagg tcatcatcgg cagcagcata
     1381 gacccgcagt tcgggccggt gctgctcttc ggcatgggcg ggcagttggt ggaggtgttc
     1441 aaggaccggg ctttgggcct gcccccgctc acgaccacgt tagcccggcg catgatggag
     1501 cgcacccaca tctacaccgc gctaaaggga gtaaggggcc gcaagccggt ggacctggag
     1561 gaggtggagc acttgctggt gcgcttcagc cagcttgtgg tggagcagcg ctggatcaag
     1621 gagagagaca tcaaccccct gctcgcctcg ccggagcggc tggtggccct ggacgctcgc
     1681 gtggtactgc acgagcctgg cgtgagcgag gaggagctac ccaggcccgc gatacgtccc
     1741 tatcccttac agtacgtgcg cgagtggact acgaaggacg gcgtgcccct gctgatacgg
     1801 cccatccgcc ccgaagacga gccgctggtg gccgagttcc acaagcgact gtctactgag
     1861 agcgtctaca tgcgctactt ccagcacgtg cagcttggcg accgcgtggc ccacgagcgg
     1921 ctggtgcgca tctgcttcaa cgacttcgac cgggaggcgg ccctggttgc cgagcacctg
     1981 gacccgcaga cggggcagcg ctcgattgtg ggcgtgggca ggctaagtcg ctcgcacgta
     2041 gcgaacgagg ccagcttcgc catcaccatc aacgacgcct ggcaggggag aggcctgggc
     2101 caggagctac tccgccgcct ggtgcagata gggcgagacg agaagctgga ccgcatcacc
     2161 gccgaaatcc tgccggagaa ccgggggatg ataagggtga gcgaaaaggt cggcttcaag
     2221 atcgtcaacg accacaaagg ggatgaagtt aaggcggtgc tggagctata gaagcgtcgg
     2281 ctcaacacaa ggcgcatgga ggaagtcccc cagcacataa gagtggccct tgtcatcctg
     2341 agcgtagcga aggatcaggt gctagagtta gacagctgga cctgtgagac tcagcacctc
     2401 ctgccacttc tggtgttgtg cgttgtagac tctcgcttac ccacctgatc cttcgctacg
     2461 ctcaggatga caaaggggag tttgggtgct cctttcggca ataaggtgcc aggccacgcg
     2521 gatgggccgc taacctcgga acttcgactc cggctggccc ttgatgccgg tgcgcagcat
     2581 gaagaggtcg cctgcaaggg gctgcttgcg gcgctgggtg acgctgaggc cgctccaggc
     2641 ggtggtgatg tagaggtcgg tcagctcggg gccgccgaag gtgcaggcgg tgacgttgct
     2701 caccggcagg tacacgtccc gttccttttt gccatccggg tcgtagcgcg tcaccctcca
     2761 accaccatag aaggcgctcc acacgaagcc ctcggagtcc accgccagcc cgtcggggaa
     2821 gccttcttcg ccggaggtgc gcacgaagtc gcggcggttc gcgatgttgc ccgtggcaag
     2881 gtcgtagtcg taggcgtaga ttacctgctt ctcggtgtcg gtgtggtaca tcgtcttgcg
     2941 gtcgggagcc cagcctatgc cgttggagat ggtgaagccg gtagccatgg tgtgcagcga
     3001 gtggtcgggg tcgaggcggt agaggctgct gccgttggag ctgccgtcga aagtcatggt
     3061 gccggcccag aagcggccgc cggggtcaac cgcgccgtcg ttgaagcgtg actctttctt
     3121 ctccaactcg gtgtcggcta caaaatcaag gcgcgacttg ctgaagtccc acagcgtgag
     3181 gccgtcgcgc accgcc
//
LOCUS       DASRWZ010000744         5875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_128606, whole genome shotgun sequence.
ACCESSION   DASRWZ010000744 DASRWZ010000000
VERSION     DASRWZ010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128606"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..852)
                     /locus_tag="VFH60_11800"
     CDS             complement(46..852)
                     /locus_tag="VFH60_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET6314509.1"
                     /translation="MIRIGTSGYSYDDWKGPYYPENIKSTEMLPFYAQEFGATEINYT
                     YYRMPTARTLAAMARKVPENFVFTVKASKELTHEREEVEEGGGTPENFRLFREALQPL
                     TDEGKFGCVLAQFPNSFKPTQENRDYLQTFRERMGDLPVVVEFRNATWVTDDTMDLLR
                     KNELGYCCVDEPRMKGLIPPIAVATAPIAYVRFHGRNAKKWWQHDEAYERYSYEYSPE
                     ELTEWVPKIEKLENTAKETFIFTNNHYRGAAVTTARQLKEMLEKAGERVV"
     gene            1341..2978
                     /gene="mazG"
                     /locus_tag="VFH60_11805"
     CDS             1341..2978
                     /gene="mazG"
                     /locus_tag="VFH60_11805"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET6314510.1"
                     /translation="MPSIIVAGLGPGAWDHVTLEVKSLLESAGTVYLRTATHPTASYL
                     PPHLDVRTFDHLYEREDRFEEIYRKIALELVSIAVSSTGAPVIYCVPGHPAIGEASVR
                     HLRALAVERGVELRLVAGLSFLEPVCTALGIDPLHRGLQIVDGTELAGFGGEKPGSAP
                     EDGDEPFLFSPYSLPVDPAAFPAMSGLTPLYPVLLCQVYNDRVASACKLMLMERYPDE
                     HPVRVVLNAGVPGQEQVISTTLAEMDRGHGINHLACVYLPALEPLHLPREFDSLVYVM
                     GRLRGPGGCPWDREQTHDSIKHHLLEEAYEAVDAMDSNDPDTLAEELGDLLLQVVFHS
                     QLAVSNEEFTLSDVVSHIVTKLVRRHPHVFGDVEVTGAEHVLRNWEVIKAGEREAKAK
                     AGRKFDSAEAANQGLEGTGLLLSVPRAMPSLDRAQQLQVRAARVGFDWSEISGIYEKV
                     QEELEELRVEESLERRTEELGDLLFVLVNLGKWLGINAEEALRRANDKFLNRFTAMER
                     AIVGEGKKLREMSVEEMDRYWDRVKAEGSAAKEGREG"
     gene            2983..3753
                     /locus_tag="VFH60_11810"
     CDS             2983..3753
                     /locus_tag="VFH60_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019494662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HET6314511.1"
                     /translation="MSKQHKYVAARPRNASLGGRRLGPKRWAYHKPVYMQSPLLTFLR
                     RLGIGALALLVVSVVTLFAYKLLVEADARTLMYKVDDPNLPHNHVALVFGAGLRPDGG
                     PSPILDDRVTAAADLYKAGKVDKLLMTGDTSQVDYNEVEAMRKAAVSQGVADRDIVLD
                     YAGFNTWDSCYRAREVFSLKEATLVTQRFHLPRALHACNYLKVKAIGVIADRQPYNTF
                     NNELRELPALAGNLFRIIINDQPRFLGPKVNVDEPQVR"
     gene            3835..4395
                     /locus_tag="VFH60_11815"
     CDS             3835..4395
                     /locus_tag="VFH60_11815"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET6314512.1"
                     /translation="MVVQIERILFTADQYEKMIEAGILEEGDNVELLRGEIVKMAPIG
                     LRHAGCVARLVSIFAAKLSASAIVWPQNPIRLADDTEPEPDVALLKPSPDFYTNRRPG
                     PQDVLLLVEVADSSLEKDQSLKVPMYAQAGIAILWIVNLIEDVVEVYASPVNGVYTEL
                     HRAGRGETILLPLAQRLSVSIDEILG"
     gene            complement(4402..4629)
                     /locus_tag="VFH60_11820"
     CDS             complement(4402..4629)
                     /locus_tag="VFH60_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314513.1"
                     /translation="MKVTYGSDANNESRAAAFYRHTTIEDLAKQQGVEPVTDPSMLAG
                     DFWPEDETAEEFIATLRQWRNEEDSRSGGNS"
     gene            complement(4786..>5875)
                     /locus_tag="VFH60_11825"
     CDS             complement(4786..>5875)
                     /locus_tag="VFH60_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET6314514.1"
                     /translation="LTIDPHLQNVAQEQLGERKGSVVVLDTQSGAILAMASWPHYDPQ
                     LLSFNPGVDDDEWEAESERIVEYYQSLLADESNPLLLRATQGLYPPGSTFKTVTAGAA
                     IDTGAAQPLSVFTDTNGEVKVEAGDYVHVDCKTCRPRNHGPQFTLTEGYKWSLNVVFA
                     ELGVQLGGNRLTEYGRRFGFGTRYDIGLPLEASRLTSNVEALQTSKNLLAATAYGQGE
                     VQATPLQMALVAAAVARGGELPAPYMVSSVNDHETHQPIWQFQPHALQQVLSPNTNEA
                     VKQMMITSIQSGWAKDAAIPGATVGGKTGTAETGRGTEHSWFIGFAGKDPAKPQYAIC
                     AMVEEGGEGTRVALPLAKVVLEEALKRK"
BASE COUNT         1073 a         1834 c         1817 g         1151 t
ORIGIN      
        1 ctttgcggtt tcatcgtctt acatgttcct ttagccgccc aggtttcaga cgacgcgctc
       61 cccggccttc tctagcattt cctttagctg ccgggcggtg gtgacggctg cgccccggta
      121 gtggttgttg gtgaagatga aggtctcctt cgcggtgttc tccagcttct cgatcttggg
      181 cacccactcg gtcagttctt cggggctgta ttcgtagctg tagcgctcgt atgcctcgtc
      241 gtgctgccac cacttcttgg cgttgcgacc gtggaagcgc acgtaagcga taggggccgt
      301 cgctaccgct atgggcggga tgaggccctt catgcgcggc tcgtccacgc agcagtagcc
      361 cagctcgttc ttgcgcagca ggtccatcgt gtcgtccgtg acccaggtgg cgttgcgaaa
      421 ctccactacc accggcaggt cgcccatgcg ctcgcggaac gtctgcaaat aatcgcggtt
      481 ctcctgcgtc ggcttgaacg agttggggaa ttgggccagc acgcagccga acttgccttc
      541 atcagtcagg ggctgaaggg cctcgcggaa cagcctgaag ttctcaggcg tgccgccccc
      601 ctcctccact tcctcccgct cgtgggtaag ctccttgctc gccttgaccg tgaacacgaa
      661 gttctccggc accttgcggg ccatggcggc cagagtgcgg gcggtgggca tgcggtagta
      721 ggtgtagttg atctccgtcg cgccgaactc ctgggcgtaa aacggcagca tctcggtgct
      781 cttgatgttc tccgggtagt acggcccctt ccaatcgtcg tagctgtagc cgctggttcc
      841 aatgcgtatc atatctctac ctccgcaacg cctgttgctt atggtgtgag ccttccgctt
      901 tttcgcttga aaacatctat ttccacacca tgtcaggcac tatttacgaa cttttgttct
      961 gattaattat accatatcag ctaccaaatt gcatccccga cgcgaaggac cataagttat
     1021 taagtcagca cctgaacagg ttactgtcat tccgagtgga gcgaggaatc aggtgggtag
     1081 ggaagaaggc gaattggagc accaggctgg ccctatgaac tctggtccca cattgcaacg
     1141 gggaacgact gccacctgat tcctcgctac gctcggaatg acaaaggggg gcgacggtcc
     1201 tgctggttgt gacacctggc ccttgcagca gcccagcagc ctactttcca ataattgccg
     1261 tatatgccgc ttcgtggtat tatccgacca gacccctcac gcacgaaagt tgaagctaac
     1321 ccctcggaag agctaatttt atgccatcga ttatcgttgc cgggcttggc cccggagcct
     1381 gggaccacgt tacactcgaa gtcaaatcgc tgctcgaatc ggcgggcacc gtctacctgc
     1441 gcaccgccac ccaccccacc gcctcgtacc tgcctcccca cctcgacgtg cgcacgttcg
     1501 accacctcta cgagcgcgag gaccgcttcg aggagatata ccgcaagatt gcccttgaac
     1561 tggtgagcat cgcggtgtcc tccacgggag cgccggtcat atactgcgtg cccggccacc
     1621 cggcgattgg cgaagcgtcg gtgcgccacc tgcgcgccct ggcggtcgag cggggcgtgg
     1681 agctaaggct ggtggcgggg ctgtctttcc tggagccggt gtgcaccgcc ctcggcatcg
     1741 acccgctgca caggggcctc cagatcgtgg acggcacgga actggcgggc ttcgggggcg
     1801 aaaagccggg tagcgcaccg gaggatggag acgagccatt cctgttcagc ccctactcgc
     1861 tgcccgtgga ccctgcggcc ttccccgcta tgtcgggcct gacccccttg tacccggtgt
     1921 tgctgtgcca ggtctacaac gaccgcgtgg cttcggcctg caagttgatg ctcatggagc
     1981 gctaccccga cgagcacccg gtgcgggtcg tgctgaacgc gggcgtgccg ggacaggagc
     2041 aggtgataag caccacgctg gccgagatgg accgggggca tggcatcaac cacctggcct
     2101 gcgtgtacct gcccgcattg gagccgctgc acctgccccg cgagttcgac tcgctcgtgt
     2161 acgtcatggg caggctgcgc ggccccggcg gctgcccatg ggaccgcgag cagacccacg
     2221 actctatcaa gcaccacctg ctcgaagagg cctacgaagc ggtggacgcg atggactcca
     2281 acgacccgga cacgctggcc gaggagctgg gcgacttgct gttgcaggtg gtgttccact
     2341 cgcagctcgc cgtctccaac gaggaattca ccctgtccga cgtggtttcg cacatcgtca
     2401 ccaagctggt gaggcggcac ccgcacgtat ttggcgacgt tgaagtgacc ggagccgagc
     2461 acgtgctgcg caactgggag gtaatcaagg cgggagagcg ggaggccaag gccaaagccg
     2521 ggcgcaagtt cgactccgcc gaggccgcca accagggcct tgagggcacc gggctgctcc
     2581 tgagcgtgcc ccgcgccatg ccctcactgg accgcgctca gcagctacag gtgcgcgcgg
     2641 cccgcgtggg gttcgactgg tcggagattt cgggcatata cgagaaggtg caagaggagc
     2701 tggaagagct gcgggtggag gagagcctcg aacggcgcac cgaggagctc ggcgacctgc
     2761 tgttcgtgct ggtgaacctg ggcaagtggc tgggcatcaa cgccgaggag gcgttgcgcc
     2821 gagccaacga caagttcctc aaccgcttca ccgcgatgga gcgggccatt gtgggcgagg
     2881 gcaagaagct gcgcgagatg agcgtggagg agatggaccg ctactgggac cgggtcaagg
     2941 ccgagggtag cgcggcgaaa gaggggagag aggggtaggc gcgtgagcaa gcagcacaag
     3001 tacgttgccg cgcgtccgcg caacgcgagt cttggagggc gcaggcttgg ccccaagcgc
     3061 tgggcgtacc acaagcccgt gtacatgcaa agccccttgc tcacctttct gcgcaggctc
     3121 ggcatcgggg cgctggcgct gctcgtcgtg agcgtggtca ccctatttgc ctacaagctg
     3181 ctggtagaag ccgacgcccg caccctcatg tacaaggtgg acgaccccaa tctgccgcac
     3241 aatcacgtcg cgctggtgtt cggggcgggg ctgaggccgg atggtgggcc ttctcccatc
     3301 ctggacgacc gtgtgaccgc cgccgccgac ctctacaagg cgggcaaggt ggacaagctg
     3361 ctcatgacgg gcgacaccag ccaggtggac tacaacgagg tcgaggcgat gcgcaaggcg
     3421 gcggtcagcc agggcgtggc cgaccgcgat atcgtgctgg actacgcggg cttcaacacg
     3481 tgggatagct gctaccgggc gagggaggta ttcagcctga aggaggccac cctggtcacc
     3541 cagcgcttcc acctgccccg cgccctgcac gcctgcaact acctgaaggt caaggcgata
     3601 ggtgtgatag ccgaccgcca gccctacaac accttcaaca acgagctccg cgagcttccc
     3661 gccctcgcgg gcaacctctt ccgcatcatc atcaacgacc agccccgctt cctcggcccc
     3721 aaggtgaacg tggacgagcc gcaggttagg tagcatattg tacgagtgta cgcttcagtg
     3781 ctatgattac cttgcgaatg gtgatgacag gttacacagg cagcaggtcc gcagatggta
     3841 gtacagattg agcgcatact gttcacagca gaccagtatg agaagatgat tgaagccgga
     3901 atactggagg agggcgacaa tgtcgagctt ctcagggggg agatcgtaaa gatggcccct
     3961 attggcctgc gccatgccgg gtgcgtggcc cgcctggttt ccatcttcgc ggccaagctc
     4021 agtgcgtcag ccatagtgtg gccgcagaac cccatacgcc tggcagacga caccgagccg
     4081 gagccggacg tagccctgct caagccaagc cccgattttt ataccaacag gcggccaggc
     4141 ccccaggatg tcctgctgtt agtggaggtt gccgacagct ccctggagaa ggaccagtcg
     4201 ttgaaggtgc cgatgtacgc gcaggctgga atagctattc tctggatcgt gaatctgata
     4261 gaggatgtcg tggaggtcta tgcaagcccc gtcaatggtg tatacacgga gcttcacagg
     4321 gcgggtcgcg gagagaccat attgctgccg ttggcgcaac ggctaagtgt gagcatagac
     4381 gaaatactgg gttaaagggt actaagaatt acccccgcta cggctatcct cctcattacg
     4441 ccactgtctc aaagtcgcaa tgaactcttc agcagtctca tcttccggcc agaaatctcc
     4501 cgctaacata ctggggtcag ttacaggctc aacaccctgt tgtttagcga ggtcctctat
     4561 agtggtatgc ctgtagaagg ctgccgccct actttcatta ttcgcatcgc ttccatatgt
     4621 gactttcatg cagaactacc accttttccg tagggacata caaatcgcag cacacggctg
     4681 gcgaccctgc tacatttcgc cgccacgtgc gtgaacctgc atgaagcctc ggccttagca
     4741 agctgacatc cctggcatgc acacaccctg gtgaaaaata gttacctact tccgcttcag
     4801 cgcctcttcc agcaccacct tagccagcgg cagggccacg cgcgtgccct cgccgccttc
     4861 ttctaccatc gcgcagatag cgtactgggg tttggcgggg tctttgcccg cgaagccgat
     4921 aaaccaggag tgttcggtgc ctcgcccagt ctcggcggtg cccgtcttgc cgcccacggt
     4981 ggcgccgggt atcgcggcgt ctttggccca gcccgactgt atggacgtaa tcatcatctg
     5041 cttgacggcc tcatttgtgt tgggtgagag cacctgctgc aaggcgtggg gctggaactg
     5101 ccatattggt tggtgggtct cgtggtcgtt caccgaggac accatgtaag gcgccggcag
     5161 ctccccaccg cgcgccaccg ccgccgccac tagggccatt tgcaggggcg tggcctgcac
     5221 ctccccctgg ccgtaggccg tggctgccag caggttcttg ctcgtctgta gcgcctccac
     5281 gttcgaggtg aggcggctgg cctccagcgg caggccgata tcgtagcgcg tgccgaaccc
     5341 gaagcgccgc ccgtactcgg tcagccggtt cccccctagc tgcaccccaa gctccgcgaa
     5401 caccacgttc agcgaccact tgtagccttc cgtcagcgtg aactgggggc cgtggttgcg
     5461 ggggcggcag gtcttgcagt caacgtgcac gtagtccccg gcttccacct tcacctcgcc
     5521 gttcgtgtcg gtgaacaccg acaggggttg ggcggccccg gtgtctatcg cggccccggc
     5581 ggtcaccgtc ttgaaggtgg agccgggagg gtagaggccc tgcgtggcgc gcaacaggag
     5641 cgggttgctc tcgtccgcca acagcgattg gtagtactcc acgatgcgct cgctctccgc
     5701 ctcccactca tcgtcgtcca cgccggggtt gaacgacagt agctgggggt cgtagtgcgg
     5761 ccacgaagcc atcgcgagga tagccccgct ctgcgtgtcg agcaccacca ccgagccttt
     5821 acgctcgcca agctgctctt gcgccacgtt ttgcaggtgg gggtctatgg tgagg
//
LOCUS       DASRWZ010000745         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_676794, whole genome shotgun sequence.
ACCESSION   DASRWZ010000745 DASRWZ010000000
VERSION     DASRWZ010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676794"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3083)
                     /locus_tag="VFH60_11830"
     CDS             complement(<1..3083)
                     /locus_tag="VFH60_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314515.1"
                     /translation="MKAIPGGDITKHRRRLFVLMAALLAVWAGAMLVGRQPEQGNPLA
                     PDTASRSDGARGAGGDPSAYGPGGSGESAELQSLEDYWLARISYPTGKVDGKWLLAAA
                     KQDKLVRNRVPGGTPDYARGRNGPNAPTSPLSMSATSWTSLGPQPLQSNGCSGCYSYG
                     HVSGRVNDIEVDPVTPNVAYIATVGGGIWKTTNCCTAATTWVPVTDDPLLNTASVDDL
                     SIDPNDHNTIYVGTGDLNFGSFSMGSVGIYKSTDAGGSWSVKGADVFGPAYPNAGNGF
                     PQYNAVGKVEADPNNSNLVVAGTKTGVYFSYNGGDTWSGPCVTNSFSSQRQDVTGLIL
                     SDSGSLTTMYVAVGARGYSTTVQSNLADNGANGIYKAVVPLTGCPLAWTSVTSPANGW
                     PAGTSTGIPQYQPGGNQLGRIDMAIAPSDANTLYAIVQAINSPGTRGAPLGVWRTTDA
                     GLTWEQRAGAGERSDSTDWQGCDNTGAQSWYNQHIEVDPNNPDVVVADTIDVFRSTNG
                     GDTFTNITCGYTQNTVKQVHVDQHALEYLPGSSSSMLAGNDGGVYVSHNMTETTPTNI
                     TFTQLNDSLNTIEFYSGDITSNFATSSSPGINAGAQDNGSMVYKWPSNNVGPAMWQVR
                     KGGDGMFARIEPMLEQNWYQESQNGNLAVTTNGPTSTLQTITGGWTADSPRVSFIFPY
                     EIYKYDCPPTGCRHLIAGSYRVWETILGGLPNGSTWYANSPDLTKNTLGDRSFINQLS
                     YAISMSTTAIVGTNDGNVQYGFNLGLGVANTANWVNVTGGNSILPNRPILDVTTDPID
                     PLIGYAAVGGFNQNTPSQPGHVFRITCTTECLTFTWQDKTGNLPNIPVDTIMVNPKYR
                     QQVFVGTDWGFYFTDDITAPSPTWSRFNTGMPNVMIWDMAIDHGFTTLAAFTRGRGAY
                     VWPLPSGPFGGPTLTPTTTATASATSVLIATQTAIETATETPSSTAIATSTATSIATE
                     TATQTATQSATETSVPTSTSTATVTSTATLTVVASASATNTTIPASSTSTSTSTAT"
BASE COUNT          524 a          870 c         1069 g          668 t
ORIGIN      
        1 gtggctgtgg aggtgcttgt ggaggtgcta gaggcaggga tggtggtgtt agtggccgat
       61 gccgaggcta ctacggtcag tgtggcggtg ctggttacgg tggctgtgct tgtggaagtg
      121 ggcacggagg tctcggtagc cgattgagtt gcggtttggg ttgccgtctc ggtagctatg
      181 ctggtagctg tgctggttgc gatagccgtg ctcgaggggg tctcggtggc cgtctcaata
      241 gctgtctgag tagcgattag cacggatgtc gcgctggccg tggcggtggt cgtaggggtc
      301 agggtggggc caccgaaggg gccggaaggc aggggccaca cataggcacc tcgcccgcga
      361 gtgaaggccg caagcgtggt gaagccgtgg tcgatcgcca tgtcccagat catcacgttg
      421 ggcatgccgg tgttgaagcg ggaccacgtg ggtgaggggg cggtgatgtc gtccgtgaag
      481 tagaagcccc agtcggtccc cacgaacacc tgctggcggt atttcgggtt gaccattatg
      541 gtgtccacgg ggatgttcgg caagttgccg gtcttgtcct gccaggtgaa agtgaggcac
      601 tcagtagtgc aggtgatgcg gaagacgtgc cccggctgcg agggagtgtt ctggttgaag
      661 ccacctacgg ccgcataccc aatcagaggg tcgatggggt cggtagttac gtccaggatt
      721 gggcggttcg gtagtataga gttgccgccc gttacgttca cccagttggc ggtattcgcg
      781 acacccagcc ccaggttgaa gccgtactgc acattcccgt cgttggtgcc cacgatggcg
      841 gttgtagaca tcgaaatcgc atagctaagc tggttgatga acgagcggtc gcccagcgta
      901 ttcttggtca ggtcggggct gttggcgtac caggttgagc cattgggcaa cccgcccaaa
      961 atggtctccc agactcgata gctgcctgca atcaggtgtc tgcacccagt cggcgggcag
     1021 tcgtacttgt aaatctcgta ggggaagatg aagctgacgc gagggctgtc ggccgtccaa
     1081 ccacccgtta tggtctgtag agtgctggtc ggaccattcg tggtgacggc aaggttgccg
     1141 ttctggctct cctggtacca gttctgctcc agcataggct cgatgcgggc gaacatgccg
     1201 tcgccgccct ttctcacctg ccacatggcc ggacccacgt tattgctggg ccacttgtag
     1261 accatggagc cgttgtcctg cgcgcctgcg ttgatgccgg gcgaagagga ggtggcaaaa
     1321 ttggaggtga tgtcgccgga gtagaactcg attgtgttca ggctgtcgtt tagctgggta
     1381 aaggtgatgt tggtgggcgt agtctccgtc atgttgtggc tgacgtaaac accgccgtcg
     1441 ttacccgcca gcatgctgga agacgagccg ggcaagtact ccaacgcgtg ctggtccacg
     1501 tgcacctgct tgacagtgtt ctgtgtgtag ccgcaggtga tattcgtgaa cgtatcgccg
     1561 ccattggtag acctgaaaac gtcaatcgta tcggccacaa ccacgtcggg gttgttggga
     1621 tcgacctcta tgtgctggtt gtaccagctc tgcgcgccgg tgttgtcgca accctgccag
     1681 tcggtgctgt cggagcgctc gcccgcgccc gcgcgctgct cccaggtaag gccggcgtcg
     1741 gtggtgcgcc acacgccgag aggcgcgccc cgggtgcctg gtgagttgat cgcctgaacg
     1801 atggcgtata aagtgttggc atcgcttggg gctattgcca tgtcgatgcg gccaagctga
     1861 ttgccgccgg gctggtactg tgggatgccg gtcgaggtgc ccgcgggcca gccgttggca
     1921 ggggaagtaa cagaggtcca ggccaggggg cagccggtga ggggtacgac ggccttgtag
     1981 atgccgttag ctccgttgtc ggcaaggttg ctctgcactg tggtgctgta gccgcgcgcg
     2041 cccaccgcca cgtacatggt ggtcaggctg ccggagtcgc tgaggataag gccggtcacg
     2101 tcctgccgct ggctgctaaa gctgttggtg acgcagggtc ccgaccatgt gtcgccaccg
     2161 ttgtaggaga aatagacgcc ggtcttggtg cctgcaacta cgaggttgct attgttgggg
     2221 tccgcctcca ccttgccgac cgcgttgtac tgcgggaagc cgttgcctgc gttaggataa
     2281 gcgggaccga acacgtccgc gcccttcacg ctccaactac caccggcgtc agttgacttg
     2341 tatatgccga cgctacccat cgagaaggag ccgaagttca ggtcacccgt gcccacgtaa
     2401 atggtgttgt ggtcgttagg gtcgatggat agatcgtcca ccgaggccgt gttgagcagc
     2461 gggtcgtcgg tgaccggcac ccaggttgtg gcggcggtgc agcagttggt ggtcttccag
     2521 atgccgccac ccacagtggc aatataggcc acgttggggg tcaccgggtc tacctctatg
     2581 tcgttgacgc ggcccgacac gtgcccgtaa ctgtagcagc cgctacagcc gttgctctgc
     2641 aagggctgcg gacccagcga ggtccaactt gtggcgctca tggatagcgg gctggtagga
     2701 gcattaggac cattccggcc tcgtgcatag tcgggagtac cgcccggcac gcgattgcgc
     2761 accagtttgt cctgcttcgc cgccgcgagc agccacttgc catcgacttt gcccgtgggg
     2821 tagctgatgc gagcgagcca gtagtcttcc aggctctgca gttctgcgct ttcacctgag
     2881 ccaccggggc cgtatgcgga ggggtcgccc cccgccccgc gagcaccgtc gctgcggctc
     2941 gcggtatcag gagcgagggg gttgccctgc tccggctgcc gccccaccaa catggctccg
     3001 gcccatacag ccagcaatgc ggccatcagg acgaacagac gccgccggtg cttagtgatg
     3061 tcgccacccg gaatagcttt catgcttcct cctcgcggaa ctctagagca ccacatacct
     3121 tcgagagtac t
//
LOCUS       DASRWZ010000746         8595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_813777, whole genome shotgun sequence.
ACCESSION   DASRWZ010000746 DASRWZ010000000
VERSION     DASRWZ010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813777"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFH60_11835"
     CDS             <1..636
                     /locus_tag="VFH60_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314516.1"
                     /translation="AVVYARVQQGLPPPVPDVPGNVLLALGVSGATTVVAKSIKVNNL
                     QQGKELGTVLPDTGPLSTLLTDDDGSLSLQKLQYLVWTFVAIGVFLVGLDQSNRLALP
                     DIDTTLLVLSGISVGAYLGPKLVPQSADEMSQIAQAAAVQGAQLSAPATMTAPSAVVA
                     STTTSGVLTPPQNPPAVVETPPNPERETAIALRLLPARLDTHPRVTYNHLE"
     gene            676..864
                     /gene="rpsU"
                     /locus_tag="VFH60_11840"
     CDS             676..864
                     /gene="rpsU"
                     /locus_tag="VFH60_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET6314517.1"
                     /translation="MTDVVVGDNESFESALRRFNKKVQQDGILAEARRREFYEKPSVL
                     RKRKEAARRRKAMLKASR"
     gene            1050..1544
                     /locus_tag="VFH60_11845"
     CDS             1050..1544
                     /locus_tag="VFH60_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET6314518.1"
                     /translation="MPLGTRHSILRLMSLKEQLTKDLQDAMRSGDELKRSTIRMVRAA
                     IKNAEIAQIGELDDAGVQNVMRKEIKQRRESADEYERHNRPELAEKERAEAALIEAYL
                     PQQMSEAEIEAVVRGIVSEVGASGPRDMNKVMPLAMARLKGRAEGRLVNKVVNGILSE
                     GAAQ"
     gene            1603..4197
                     /locus_tag="VFH60_11850"
     CDS             1603..4197
                     /locus_tag="VFH60_11850"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET6314519.1"
                     /translation="MDSPSQPDMPRPRPTPVESDPRPASPQKPASSGGRRQGHHSSGA
                     TRFRLPFRARPVSPIAGEAEPYRGRPRHVVFSAIFLVIALTAIMVVPFQPGQDSIEPG
                     LPAGQDIFSPIFLRYESKVLTDDARNKAMQLPSNEVWVQDGEVVQRQRFILLNNLSAI
                     DSVRNNQDPNPNPGLNRLHSLQDTPLTQAQIDLLLEMNDSDYRYWRENGVIAGFDAAM
                     KSRRITSDADLNAVRAALPDLLVPSLTAEQKAVALAFIVPRLSVNMTLDEAQTEQRRQ
                     NAASNVKPVVVTVQRGETILRQGELITPEAVEKLQEAGLLSRQLSPQSVLGTAGIVGL
                     LMLLLHIYIFRNAPQVWRRHKQLVLVAMLLIITVGAARLILPGHSLLPYLLPVAAVSM
                     LIAVLLSADLAVVVTVVLSVLLGVMLSNGLSMDLPVYYFVSGLTGIFSLTRIEKVSTF
                     ARAGFFIAGASFASALVTQVLSVGVPDWYSVGLLMLAAAFNGGIATSITYAAFSLLGT
                     LFGITTPLQLMELAHPDQPLLRRLMQEAPGTYHHSLVVSNLAERAAEMIGADTLLARV
                     CAYYHDIGKVEHPEAFIDNQSGMANLHDRLSPADSAQIIAGHVRDGVKLGQKYKLPKR
                     VLDAIPQHHGTMLIKYFYYKAAAADPYVSQDDFRYPGPRPQTKENAILMLADGVEATV
                     RSMAQSGALDKMATAAVAATANSDATESPSLYNNAASLPPDAIAEVVHKIISERIEEG
                     QLDECDLTVRDLARIQEAFVSMLKGIYHPRIVYPEPPNKQAAQAPAPAEATPVATPSA
                     VLVAVPSGGASTDAANGTNGANGTNGANGTNGANGHGAYGHEAPSTETTPTTESQHAT
                     RPSTSPLL"
     gene            4163..4717
                     /gene="ybeY"
                     /locus_tag="VFH60_11855"
     CDS             4163..4717
                     /gene="ybeY"
                     /locus_tag="VFH60_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET6314520.1"
                     /translation="MQPGPQPPPSSEERNANIPDTQGAQVSADPGSGYLITVQVEPQF
                     EDKVDADALHRVAMHVLKSEGAPTPLEVGVVVTTDDEVHALNRQYLGHDYQTDVISFG
                     MAAEGSEEFITPEERPRYLGDVVVSYDRAAEQAPEYGHDPAREVATLLVHGLLHLLGY
                     DDTTDADREKMHQRQDALIVDFGF"
     gene            4747..5181
                     /locus_tag="VFH60_11860"
     CDS             4747..5181
                     /locus_tag="VFH60_11860"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET6314521.1"
                     /translation="MTDPGPPLPPDSLVPSKSKIQNPKPKIVSSFGFAFAGLAYVFRT
                     QRNFRIHIAIALLMSLMGWLLGLSLAEWAVFGVMVVVVLAAEMTNTMVEALVDLVSPG
                     YHPLAKVSKDVAAGVVLLTAIGAVVVGLLIFGPKLLAAVGLV"
     gene            5240..5533
                     /locus_tag="VFH60_11865"
     CDS             5240..5533
                     /locus_tag="VFH60_11865"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET6314522.1"
                     /translation="MSKTVNLIEAQDSLSELADEANAGQTIIVANEGQEVAVILGIEE
                     YRRLKNVEGEQRSRDFGVLLSPMDRDGLSEEEAREIAVEAVREYRAANHTTEA"
     gene            6070..7359
                     /locus_tag="VFH60_11870"
     CDS             6070..7359
                     /locus_tag="VFH60_11870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6314523.1"
                     /translation="MLAPQWPDPARQGAAIRNLQVLLHLAQRHEVYLLTFASDGPMEM
                     ERLRQACVLAEALPRPTRSSILRLQQLATTRLPDMAWRLYSDEMLQRVTSLCQEVPFD
                     AVHIEGIEMATYGLLAARFRIGDFGMWTEELRPDSFGGYSMQLRKLESPTPSLTYDAH
                     NAEYVLQRRAFTTDVTDPARLPHAMYSLVQWWRLRRFERRVCIASKDILAVSDADRKA
                     LALRAPDKAAAMRVLPNGVDPNYWSRDAAFSPANIPRGVEALVFDGTMDFRPNVDAAL
                     WFVKRVLPLVRADRPQVRFYIVGRNPSPEVVALADTPGVTVTGAVPDTRGWVARATVY
                     VVPMRMGGGVRLKVLQAMSMGCAIVSTPMGAEGIFIRPNKDVMLARTPIDFALNILTL
                     LQDRSQRVELGISARERALDRYTWDKLLPTLDEIYPT"
     gene            complement(7769..>8595)
                     /locus_tag="VFH60_11875"
     CDS             complement(7769..>8595)
                     /locus_tag="VFH60_11875"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET6314524.1"
                     /translation="LLADIVAWHIRGTHFGIKVDPEDGLLTAGEEGLQLTWMDAKVDD
                     WVVTERRGKPVEINALWYNALRVMDKLRVSLGRTMVRGGKEELPDFHALANQVRDSFR
                     TRFWYGAGDYLFDVIDGPGGHDTSIRPNQIFAVSLRDDLLSVDQQRLVLECVRKHLLT
                     PYGLRTLSPEDPHYIANYTGNRRERDGAYHMGTVWPWLLGAYFDAVRNVEGAEAAAGE
                     LRSMLPTLRRHLAEAGLGSISEIFDADPPHAPRGTISQAWSVAELLRHLWGPSRLL"
BASE COUNT         1675 a         2747 c         2637 g         1536 t
ORIGIN      
        1 gcggtggtgt acgccagggt ccagcagggg ctgcctcccc ccgtcccgga cgtacccggc
       61 aacgtgctcc tggcgttggg ggtaagcggc gctacgaccg tggtggccaa gagcatcaag
      121 gtgaacaatc tacagcaggg caaagagctt ggcaccgtac tgcccgacac cggccctctc
      181 tctacgctgc tgacggacga cgatggctcg ctcagcctgc aaaaactgca ataccttgtg
      241 tggaccttcg tagccatcgg cgtcttcctg gtcggcctgg accagagcaa ccgcctggcg
      301 ctacccgaca tagacaccac gctgctggtg ctgtcaggta taagcgtggg cgcctacctt
      361 ggcccgaagc tggtgccgca gtcggcggac gaaatgtccc agatagcgca ggccgcagcc
      421 gtccaggggg cgcagcttag cgcaccggcg accatgactg ctcccagcgc cgtagtagcg
      481 agcaccacga cgtccggcgt cctaacgccc cctcagaatc cacctgcggt tgtggagaca
      541 cccccgaacc ctgagaggga gacggcgata gccctgcggc tacttccagc ccggcttgac
      601 actcacccac gcgtgacata taatcatctg gaatagcaag taccaggcat accgtggcga
      661 aaggggtgat gtaacgtgac tgacgttgta gtcggcgata acgagagttt cgagagcgca
      721 ctgcgccgat tcaacaagaa ggtgcagcag gacggcattc tggccgaggc tcgccggcgc
      781 gagttctacg agaagccgag cgtgctgcgc aagcgtaaag aagccgcccg ccgccgcaag
      841 gctatgctga aggcctcccg ataacatctt ctgtttgaac gtaccagggt ctaggagtgc
      901 gatgtgcgaa ttagtgcagt gccgccccta gacccttgtg ctttctgaaa agtaccgagt
      961 gccgagtacc gagtaccgag taagaagacg aaaacgcaaa gacgcaaaga acgtaaagaa
     1021 cgcgcaaaga ttaaggattg tttcaacggg tgccactcgg cactcggcac tcgatactta
     1081 gacttatgag cctcaaagag caactaacca aagacttgca ggacgctatg cgtagcgggg
     1141 acgagctaaa gaggtccacc atacgcatgg tgcgcgcggc tataaagaac gccgagatcg
     1201 cgcagatagg cgagcttgac gacgcgggcg tgcagaacgt catgcgcaag gagatcaagc
     1261 agcgccgcga gtctgccgac gagtacgagc ggcataacag gcccgaactc gcggagaagg
     1321 agcgggccga ggccgccctt atcgaggctt acctgcccca gcaaatgtcc gaagctgaga
     1381 tcgaggccgt ggtacgcggc atcgtcagcg aggtgggcgc gagcggcccc agggacatga
     1441 acaaagtgat gcccctcgcc atggcccgcc tcaagggacg cgccgagggc aggctggtca
     1501 acaaagtcgt taacggcata ctctccgagg gcgcagccca gtagctgccg gcgcgtagct
     1561 gccagcggcg ctttctcccg gagacctgaa gcagcaaggc ctatggacag ccctagccag
     1621 cccgacatgc cccgacctcg acccacgccc gtggagagcg atccgcgtcc cgcatccccc
     1681 caaaagcccg cttcctccgg gggcagacgg caaggccacc attcttcggg cgcgacgcgg
     1741 ttccggctgc ccttccgtgc tcgtcccgtc agccccatag cgggcgaagc cgagccctac
     1801 agggggcgtc cccgccacgt cgtcttctcg gctattttcc tcgtcatcgc cctcaccgcc
     1861 atcatggtgg tgccgttcca gccgggccag gacagcatag agcctggctt gcccgccggc
     1921 caggacatct tttcgcccat cttcctgcgc tacgagtcga aggtgctcac cgacgacgct
     1981 cgcaacaagg cgatgcagtt accctccaac gaggtgtggg tgcaggacgg cgaggtggtg
     2041 cagcgccagc gctttatcct gctcaacaac ctctcggcaa tcgactcggt gcgcaacaac
     2101 caggacccca atcccaatcc cggcctcaac cgcctccaca gcttgcaaga caccccgctg
     2161 acccaggcgc agatagacct gctgttggag atgaacgaca gcgattaccg ctactggcgc
     2221 gagaacgggg tgatagccgg gttcgatgcc gcgatgaaga gccgccgcat caccagcgac
     2281 gccgacctca acgcggtgcg cgcggccctg ccagacctgc tggtgccctc gctcaccgcc
     2341 gagcagaagg ccgtggccct cgccttcata gtcccgcgcc tctccgtcaa tatgacgttg
     2401 gacgaggccc agaccgagca gcgccgccag aacgccgcga gcaacgtcaa gccggtggtg
     2461 gtcacggtgc agcgcggcga gacgatcctg cggcagggcg agctgataac cccggaggcg
     2521 gtcgagaagc tacaggaggc gggcctcctc agcaggcagc tatcgcccca aagcgtgctc
     2581 ggcacggcgg gcatagtggg cctgctcatg ctcctgctgc acatctatat cttccgcaac
     2641 gccccccagg tgtggcggcg tcacaagcag ttggtgctgg tggcgatgct gctcatcatc
     2701 acggtggggg cggcccgcct gatactgccg gggcactcgc tgctgcccta tttgctgccc
     2761 gtggccgccg tatcgatgct catagcggtg ctgctcagcg cggacttggc ggtagtggtc
     2821 accgtggtgc tgtcggtgct gcttggggtc atgctcagca acggcctatc gatggacctg
     2881 cccgtgtact atttcgtcag cgggctgacg ggcatattca gcctcacccg catcgagaag
     2941 gtgagcacct tcgccagggc gggcttcttc atcgcgggcg cgtcgttcgc ctcggccctg
     3001 gtcacgcagg tgctctccgt gggcgtgccc gactggtact cggtggggct gctcatgctg
     3061 gcggcggcct tcaacggcgg catagcgacc tcgatcacct acgcggcctt ctcgctgctg
     3121 ggcaccctgt tcggcatcac cacgcccctg caattgatgg agcttgccca ccccgaccag
     3181 ccgctcctgc gccgcctgat gcaggaggcc cccggcacct accaccactc cctcgtggtg
     3241 agcaacctgg ccgagcgcgc cgccgagatg atcggggccg acacgctcct ggcgagggtg
     3301 tgcgcctact accacgacat cggcaaggtg gagcacccgg aggctttcat agacaaccag
     3361 tcgggtatgg cgaacctgca cgaccgcctc agccccgcgg acagcgccca gatcatcgcg
     3421 gggcacgtgc gcgacggggt gaagctgggg cagaagtaca agctgcccaa gcgcgtgctc
     3481 gacgccatac cgcagcacca cggcacgatg ctcatcaagt acttctacta taaggccgcc
     3541 gcggcagacc cctacgtgag ccaggacgac ttccgctacc ccggcccgcg cccgcagacc
     3601 aaggagaacg ccatcctgat gctggccgac ggggtcgagg ctactgtgcg ctcgatggcc
     3661 cagagcggcg cgctcgacaa gatggctacc gcggccgtgg cggccaccgc caacagcgac
     3721 gctaccgaaa gcccctccct ctacaacaac gccgcctcac tgccgcccga cgctatcgcg
     3781 gaggtagtgc acaagatcat cagcgagcgc atcgaggaag gacagcttga cgagtgcgac
     3841 ctcacggtgc gcgacctcgc ccggatacag gaggcgttcg tcagcatgct caagggcatc
     3901 taccacccgc gcatcgtcta ccctgagcct cccaacaagc aggctgccca ggcccccgca
     3961 cctgctgaag ccacacctgt cgctaccccg tctgccgtgc tggtagccgt gccaagcggt
     4021 ggtgctagca cggacgccgc taacggcacc aacggcgcta acggcaccaa cggcgctaac
     4081 ggcaccaacg gggcgaatgg acatggcgca tacggccacg aggcaccttc caccgagacc
     4141 acccccacca cggagagcca gcatgcaacc cggccctcaa cctcccccct cctctgagga
     4201 gcgtaacgcc aacatacccg atacgcaggg cgcgcaggtc agcgcggacc ccggctccgg
     4261 ctacctcatc acggtgcagg tggagccgca gttcgaggac aaggtagacg ccgacgcgct
     4321 gcacagagtg gcgatgcacg tcctcaagtc tgagggtgcc cccacccccc ttgaggtggg
     4381 cgtggtggtg accacggacg acgaggtaca cgccctcaac cgccagtacc tcggccacga
     4441 ctaccagacc gacgtgatct ccttcggcat ggccgcggaa ggcagcgagg agttcatcac
     4501 cccggaggag cgtccccgct acctgggcga cgtggtcgtc tcatacgacc gcgccgccga
     4561 gcaagccccc gaatacgggc acgaccccgc ccgcgaggtc gccaccctgc tggtgcacgg
     4621 cctcctgcac ctcctcggct acgacgacac caccgacgcc gaccgcgaga aaatgcacca
     4681 acggcaagac gcacttattg tcgattttgg attttaggtt ttggattttg gattggctga
     4741 aacgaagtga ccgaccctgg acccccctta ccgcctgact cgctagtccc gtccaaatcc
     4801 aaaatccaaa atccaaaacc taaaatcgtc tcttccttcg gcttcgcatt tgcggggctt
     4861 gcctacgtct ttcgtacaca gcgcaacttt cgcatccata ttgctatagc cttgctcatg
     4921 tcgctgatgg gctggctgct gggtctgagc ctggcggagt gggcggtgtt cggggtgatg
     4981 gtggtagtgg tgctggcagc cgagatgacc aacacgatgg tcgaggccct ggtggacctg
     5041 gtgtcgcccg gctaccaccc ccttgctaag gtgtcaaagg acgtagcggc gggcgtggtg
     5101 ctgctcacgg cgataggggc agtggtagtg gggctgctga ttttcgggcc taagttgctg
     5161 gcggcggtgg gactggtgta gcgcgcggta tgttgttttc gtggcctgtg ttacaattct
     5221 gtatgaaagg gcatctattg tgagcaagac agtcaatctc atcgaagcac aggatagtct
     5281 gtccgaactg gctgacgaag cgaatgctgg tcagactatc atcgtggcga atgaaggcca
     5341 agaagtagcc gtgattctgg gcatagagga gtaccggcgg ctaaagaacg tggaagggga
     5401 acagcgcagc cgcgatttcg gtgttctatt gtctccaatg gaccgagatg ggctttctga
     5461 agaggaggcg cgagaaatcg ctgtcgaggc tgtgcgcgag taccgggccg ccaatcacac
     5521 taccgaagcc tgatgcgcgt ggttgtagac actaatgtgt acgtgtctta tttgttgtcg
     5581 cccagaggaa ctactgtttg gttactgtct ctatggcgtg atgggcattt cgacgtagca
     5641 atctcacctt cattatttgc ggagttggtg ggagtacttc aacgctctca catctcggag
     5701 cgaattgacc ttcaacgtcg ctttagcttg ctccggcgct tacggcagga agctgtgtgg
     5761 acgcccggcg aactggatgc ctccggtgca acccctgatc ctaaagatga tatgctggtc
     5821 agcactgctc tggaaacggg tgctcagtat gtagtcacct gggatgcagc gcttctaagt
     5881 caggtcactc gtgatgacct gcgcctggtt actcctgaag agcttattgc ccttgtgtcg
     5941 ccaaggtaat cagtttagtc ggaacccagt tatctactag ggctttgcca gcatttcgcc
     6001 gtctctggta gatggtgtgc cacttcattc tcagcacact aagttataat acccacgatg
     6061 agaatcctag tgctggcccc ccagtggccc gaccccgccc ggcagggtgc ggctatccgc
     6121 aacctgcaag tgctgttgca cctcgcgcag cggcacgaag tctacctgct gacgttcgcc
     6181 tccgacggcc ctatggagat ggagcggctg cggcaggcgt gcgtgctggc cgaagccctg
     6241 cctcgcccta cccgctccag catccttcgg ttgcagcaac tggctaccac tcgcctgccc
     6301 gacatggcgt ggcggctcta ctcggacgag atgctccaac gcgtcacgtc cctgtgccag
     6361 gaggtgccgt ttgacgcggt gcacattgag ggcatcgaga tggctaccta tggcctactc
     6421 gccgcccggt tccgcatcgg ggatttcggc atgtggaccg aggagctacg ccccgacagc
     6481 ttcggcggct attcgatgca gcttcgcaag ctggagtcgc ctacgcccag cctgacctac
     6541 gacgcccaca acgccgagta cgtcttgcaa cgccgcgcct tcacgaccga cgtcaccgac
     6601 cccgcccggc tgccccacgc tatgtactcg ctggtgcagt ggtggcggct gcgcaggttc
     6661 gagcggcggg tgtgcatcgc cagcaaggac atactggccg tctcggacgc cgaccgcaag
     6721 gcgctggccc tccgtgcgcc ggataaggcg gctgccatgc gcgtgctgcc caacggggtc
     6781 gatcccaact actggtcgag ggacgccgcc ttctcccccg ctaacatacc gcgcggggtg
     6841 gaagcgctgg tgtttgacgg cacaatggac ttccgcccta acgtagacgc ggcactgtgg
     6901 ttcgtaaagc gggtgctccc cctcgtcagg gccgaccgcc cgcaggtgcg cttttacata
     6961 gtggggcgca acccctcacc ggaggtggtg gcgctggccg acacaccggg ggtgaccgtc
     7021 acgggcgcgg tgcccgacac gcggggctgg gtggcgaggg ctaccgtcta cgtggtgccg
     7081 atgcgcatgg gcggtggcgt gcggttgaag gtgttgcagg ctatgtcgat gggctgcgcg
     7141 atagtgtcca ccccgatggg tgcggaaggt atcttcatac gccccaacaa ggacgtcatg
     7201 ctggcccgca ccccgattga cttcgccctc aacatcctaa cgctgctaca ggaccgctcg
     7261 caacgcgtcg aactcggcat ctccgcccgc gagcgcgccc tcgaccgcta cacctgggac
     7321 aaactcctcc ccaccctgga cgaaatatac ccaacctgat gatgtaaata gacggaaacg
     7381 caaagacgca aagaacgcgc agagagaaga atcgtttaga gttgtagggc tacgatgcat
     7441 cctgccctgg taggtagcat cgatgcttct ttcctaagga ctgaaccacg tgttgtcatc
     7501 ctgagcgtag cgaaggatca ggtgggtagg ccagagtctt caacattgag gatgatgtgg
     7561 gtaagaggaa cactggtgca gcaataggga gaatcaagaa cgggcggctg atccttcgct
     7621 acgctcagga tgacaagggg gagtttgatc ctctggaaag aagcacatcc tcgaagacac
     7681 caggtatagc actcctacac acatcctaac ccttaatagt ctttgcgcgt tctttgcgtc
     7741 tttgcgtctt tgcgtttccg tctaaatctc agaggaggcg ggagggaccc cagaggtggc
     7801 gcaggagttc ggctacgctc caggcctggg agatagtgcc cctgggggca tgaggtgggt
     7861 ccgcgtcgaa gatttcgctg atggagccta gcccggcctc tgccaggtgg cggcggaggg
     7921 tggggagcat gctccgtagc tctccggctg ctgcctccgc gccctctacg ttgcgcaccg
     7981 cgtcgaaata ggcccctagc agccacggcc acacagtgcc catgtggtac gcaccatccc
     8041 gctctcgccg gttgccggtg tagttggcga tataatgcgg gtcttcagga gagagggtgc
     8101 ggaggccgta aggggtgagc aggtgcttgc gcacgcactc caacacgagg cgctgctggt
     8161 caacggagag caggtcgtcg cgcagagaga cggcgaagat ttgattgggg cggatggagg
     8221 tgtcatggcc ccccggaccg tctatcacgt cgaacaggta atcgcccgcg ccgtaccaga
     8281 agcgggtgcg gaacgagtcg cgcacctggt tggcaagggc gtggaaatcg ggcaactcct
     8341 ccttgccgcc cctcaccatc gtgcggccca gcgacactcg cagcttgtcc atcacgcgca
     8401 gggcgttgta ccagagggcg ttgatctcta cgggcttacc ccgccgctcc gtcaccaccc
     8461 agtcgtccac cttggcgtcc atccaggtaa gttgcaagcc ctcttcgccc gccgtgagca
     8521 agccgtcctc cgggtctacc ttgatgccga agtgcgtgcc ccgtatatgc caggccacaa
     8581 tatcggccag cagcg
//
LOCUS       DASRWZ010000747         5044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_677183, whole genome shotgun sequence.
ACCESSION   DASRWZ010000747 DASRWZ010000000
VERSION     DASRWZ010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677183"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..3318)
                     /locus_tag="VFH60_11880"
     CDS             complement(166..3318)
                     /locus_tag="VFH60_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314525.1"
                     /translation="MSLKSGGREPSFYTERGPASGGGRLVRSLALFWGLLSLCVAALV
                     LAGALPVGAKEPVTEAPGKAQSKPTTPGYGSMPVAAYSDAAGPAGVESAAPDMEGGPD
                     AFGYTFADERDPGGPVYNFREGTTRIADSAWLTPTAIPNTGGPLDDGVTSVNLPFPFT
                     FYGVTYNQVNISTNGNIHFGQPNAWVPGSDCLPSSSQFVPKAMLAPLWYDFRVPTAAS
                     NAGGVYVGSEGTSPNRSYIIEWRNVPNYDEDSIRATFEVILYENGQATFQYKDLVGEG
                     VSGSQAVVGIQNDNGSVGLKYLCRENVLVPNRAIRFRLVQDVFLQPSTASKGGAPGAT
                     FTYTETLRNQTGINNSFVLTYTGNLWPTTVEPLTTGTIANGASMPVTVTVQIPPGSPI
                     GSYDDVTVSASSTLPSPGAFTATAVLTTSVNTTGVDFSPKNPTKGGDFNSTVTHSMRL
                     FNRSGQTNSFDVQKQQSGSGWSTSLTPSNTGSMPNESSVPVTLTVNVPSNARLGDSEV
                     VTISASSDLPLPGSYYGEQVVTTTAGVWLNRTDLMQPRSRSAAVSFGNQGHIYVLGGE
                     NPEGDIDLPVELYDSLSNRWYSRAHLAQPVSNAGAAAIGDAIYVVGGTSGAEAQSTLQ
                     VYRPGTDATEIVSTDPLPQPRFGAGVVAVNGKLYVIGGADSDQIGTNTVFEYDPALPA
                     GSRWRSRAAMPTARMYLGASAVDGTIYAVGGAARAVPVADLAKVEAYNPGTNSWTTLA
                     SMSRGRAGVTAVGVNSGEPGCGARLYALGGGYTQPSATGEVYDPSTGKWRPLSNMVLG
                     RRTHAAAYNPYTKSLMVFGGWINNVESEVESISCMSGVIYCEAGFGDVPPDSPFYAYV
                     RCLACRSIVGGYADGTFGPNNPVTRGQLSKIVSNAAGFSEEHEGQTYQDVSPDSPFYI
                     WVERLSSRSIIGGYPCGEPGESCEPDGRAYFRPNANSTRGQITKIVANAAGFNEPVPA
                     GSQTFTDVAPGSPFYEFVQRLTSRSVMAGYPCGGAGESCDGQNRPYFRPTANATRGQI
                     SKIVANTFFPGCETSTQPSD"
     gene            3597..4868
                     /locus_tag="VFH60_11885"
     CDS             3597..4868
                     /locus_tag="VFH60_11885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6314526.1"
                     /translation="MSATTTTTYVTTEVPAFVRRNTFLLAVAQCTGWLGLQMMATLSS
                     IVAYELTRDPQWAGIPTTLFAVSSALAAPFAGRLTDRLGRKPVLASGQALAGLGSLAA
                     GLSIPAGTLVGFLLGILLMGAGTGAVLLARSAAADMYPASKRAGGMSLVIMGGAAGAI
                     FGPSLLGLVTGSSTTGLQIALPWLILPVLNVVAIAAIAAIRPDPRQIAADLERYYPGE
                     TPAREAAFEAAPPRTLSEVLRQPAVIVAVASTALVQAGMVMLMVTASLSLKLHGHGES
                     VYGVMTGHFVGMLGLSLLIGRIADRLGRRAVIMGGALIYVSGALTAPLFENPLYNGAS
                     LFMVGLGWSLCYVAGNAVLADVSRASERGRTMGANDLLVGLTGAFASLTGGLLLGGAG
                     YLTVGAVGLALGVVPFLLALRLREPSPGRYE"
BASE COUNT          872 a         1587 c         1640 g          945 t
ORIGIN      
        1 cagagcaaaa ggaccagggt acatgttgtc gtaccctggt cctctttgca ccacctaaac
       61 ctttattctc tctgcgtaac ctctgcgtcc tctgcgtctc tgcggtgaac cgttctttat
      121 cttggcgcgc tcttggcgtc cttggcgtct tggcggtttc gtctcctagt cggagggctg
      181 ggtggaggtc tcgcagccgg ggaagaaggt gttggctact atcttggaga tttggcccct
      241 ggtggcgttg gcggtggggc ggaagtaggg gcggttttgc ccgtcgcacg actcgcccgc
      301 tcctccgcag gggtagcccg ccatgacgga gcggctggtc aagcgctgca cgaactcata
      361 gaaagggctg cctggggcca cgtcggtgaa ggtctggctg cccgctggca ccggctcgtt
      421 gaagcccgcc gcgttcgcca cgatcttggt aatctgcccg cgggtcgagt tggcgttggg
      481 gcggaagtac gccctgccgt ccggctcgca cgactctcca ggctccccgc acgggtagcc
      541 accgatgatc gagcgactcg acaggcgctc cacccagatg tagaaggggc tatcgggcga
      601 cacgtcctgg taggtctgcc cttcgtgctc ctcgctgaag cccgccgcgt tgctcacgat
      661 cttcgatagc tggccgcgcg ttaccgggtt gttcgggccg aaggtgccgt cagcgtagcc
      721 gccgacgatg ctgcgacacg ccaggcaccg cacgtatgcg tagaaggggc tgtccggcgg
      781 cacgtccccg aaccccgcct cgcagtaaat cacgcccgac atgcaggaga tcgactctac
      841 ctcgctctcg acgttgttga tccacccgcc gaacaccatc agcgacttcg tatatgggtt
      901 ataagcggcg gcatgcgtgc gccggccgag gaccatattc gataagggcc gccatttgcc
      961 cgtagagggg tcgtacacct cgcccgtggc gctaggctgg gtgtagccgc cgcccagggc
     1021 gtacaggcgc gctccacaac cgggctcgcc ggaattgacg cccacggccg taacccctgc
     1081 cctgccccgg ctcattgaag cgagcgtggt ccagctatta gtgccggggt tgtaggcctc
     1141 caccttagcc aggtccgcga ccgggaccgc gcgggcagcg ccacccaccg cgtaaatcgt
     1201 gccgtccacc gccgatgcgc cgaggtacat acgggccgtg ggcatggcag ccctcgaccg
     1261 ccagcggctt cccgccggga gcgcggggtc gtactcgaac accgtattgg tgcctatctg
     1321 gtcggagtcc gcgccgccta ttacgtacag cttgccattc accgccacca cgcccgcccc
     1381 gaagcgaggc tggggcagcg ggtcggtgct tacgatctcc gtagcgtccg tgccgggcct
     1441 gtagacttgc aacgtactct gcgcttcggc cccgctcgtg ccgcccacca cgtaaatggc
     1501 gtcgccgatt gccgccgctc ccgcgttgga gaccggctgc gccaggtgcg cgcgtgagta
     1561 ccagcggttg ctaaggctgt cgtaaagctc caccggcagg tcgatgtcgc cctccgggtt
     1621 ctcgccgccg agcacgtaga tgtggccctg gttgccgaag ctcacggcag cggagcggct
     1681 gcgaggctgc attaagtcgg tgcggttgag ccacaccccc gccgtggtgg tcactacctg
     1741 ctcgccgtag tagctccccg gcaacggcag gtcggacgac gcgctgatgg tcactacttc
     1801 gctgtcaccg agccgggcgt tggagggcac gttcacagta agcgtaaccg gcaccgaact
     1861 ctcgttgggc atggagccgg tgttggaggg cgtgaggctg gtgctccaac ccgaccctga
     1921 ttgctgcttc tgcacgtcga agctattggt ctggccgctg cggttgaaca ggcgcatcga
     1981 atgggtgacg gtgctgttga aatcgccgcc cttggtaggg ttcttcgggc tgaagtccac
     2041 gccggtcgta ttgaccgacg tggtaagcac cgccgtggcg gtaaacgcac ccggcgaggg
     2101 cagggtagac gaggcagaga ccgtcacgtc gtcatacgag cctatgggcg agcctggcgg
     2161 tatctgcacc gtaaccgtaa cgggcatgga agcgccattg gctatcgtgc ccgtagtgag
     2221 cggctcgacc gtagtgggcc acaggttgcc ggtgtaggtc agcacgaacg aattgttgat
     2281 gccggtctgg ttacgaaggg tctcggtgta cgtgaaagta gccccaggag cgcccccttt
     2341 cgaagccgtg ctgggctgca agaagacatc ctgcaccagg cggaagcgta tagcccggtt
     2401 gggcaccagc acgttctcac ggcagaggta cttcagacct acggacccgt tgtcgttttg
     2461 aatgcccacc acggcctgtg agccggagac cccctctcca accagatctt tgtactggaa
     2521 ggtagcctgg ccgttttcat agaggatcac ctcgaaggtg gccctgatgg aatcttcgtc
     2581 gtagttgggg acgttgcgcc actcgatgat gtaagatcga ttgggagagg tgccttcgga
     2641 tcccacatat acgccgccag cgttactggc tgcggttgga actctgaagt cgtaccaaag
     2701 gggggcaagc atagccttgg gaacgaactg gctgctggat ggcaaacagt cgctgccagg
     2761 cacccaagcg ttaggctggc cgaaatggat attgccgttt gtgctgatat ttacctggtt
     2821 gtaggtcaca ccgtaaaaag taaaaggaaa aggcaggttg acgctcgtca cgccatcgtc
     2881 aagaggccca ccagtattcg ggatggcagt cggtgtcagc caggcgctgt cggcaatcct
     2941 ggtggtgcct tcgcggaagt tatataccgg gccgccgggg tcgcgctcgt cagcgaaggt
     3001 gtagccgaaa gcgtcggggc caccctccat gtcaggcgca gcagactcca cacccgccgg
     3061 gccagccgca tccgaatagg ccgcgacggg catcgagcca tagccaggag ttgtgggctt
     3121 gctctgtgcc ttgccgggag cctccgtaac aggctctttg gcacccaccg gcagggcacc
     3181 cgcgagcacc agcgcagcca cgcacagcga cagcaacccc cagaacaaag ccagggaccg
     3241 aacgagacga cccccgccgg aggctggccc tctctctgta tagaaggaag gctcccggcc
     3301 tcccgacttg agtgacatag tagctggact ccttacatcg ccggtggtaa gatagcatac
     3361 aaacaacgga gcgatatacg ggagtcgaag tacctatata tgcgctcgac aacgccgggt
     3421 ctgccaggta attgtccaag acaggcggcg gtttgtcaaa tgggacgagt gccgagtacc
     3481 gagtgccgag taccgagggg cactgatcga aacaaccctt aatctctgcg cgttctttgc
     3541 gccctttgcg tctttgcgct ttcgtttcct tactcggtac tcgacactca ccccctatgt
     3601 ccgctacgac caccacaact tacgtcacga ccgaagtgcc tgccttcgtg cgacgtaaca
     3661 cgttcctgct ggccgtcgcg cagtgcaccg gctggttggg gttgcagatg atggcaaccc
     3721 tgagtagcat cgtggcgtac gagttgacca gggacccaca gtgggcgggc attcccacca
     3781 cgctgttcgc cgtgtcctcc gccctggcgg caccgttcgc cgggaggctg acggataggc
     3841 tcggcaggaa gcccgtgctc gcgtcggggc aggcgttggc gggcctgggc agtttggcgg
     3901 cggggctgtc gataccggca ggtacactcg tgggcttcct gctgggcata ctgctcatgg
     3961 gcgcgggcac gggcgcggtc ctgctggcca ggtcggcggc tgcggacatg tatcccgcgt
     4021 cgaagcgtgc ggggggcatg agcctggtga ttatgggagg ggcggcgggg gccatattcg
     4081 gaccttccct cctggggctg gtcaccggtt ccagcacaac ggggctgcaa atcgccctgc
     4141 cctggctcat cctgcccgtc ctcaacgtcg tggctatcgc cgccatagcc gccatacgcc
     4201 ccgacccccg ccagatagcc gccgacctcg aacgctacta ccccggcgag acgcccgcac
     4261 gggaggccgc gttcgaggcg gccccaccca ggacgttgag cgaggtgctg cgccaacccg
     4321 ccgtgatagt ggcggtggcc tccaccgcgc tggtgcaggc tggcatggtg atgctgatgg
     4381 tgacggcctc gctgagcctg aagctacacg ggcacggcga gtcggtgtac ggggtgatga
     4441 cgggccactt cgtgggcatg ctggggctgt cgctgctgat tggccgcatc gcggaccggc
     4501 tcggcaggcg ggcggtcatc atgggcgggg cgctcatcta cgtctccggc gcgctcacgg
     4561 ctcccctgtt cgagaacccc ctctacaacg gcgcgagcct gttcatggtg ggcctggggt
     4621 ggagcctctg ctacgtggcg ggcaatgccg tgctggccga cgtgagccgc gcctcggagc
     4681 ggggccgcac gatgggcgcg aacgacctgc ttgtgggcct tacgggcgcg ttcgcatcgc
     4741 tcaccggcgg cctgctgcta ggcggcgcgg gctacctcac agtgggcgcg gtgggcctgg
     4801 ccctgggtgt ggtgcccttc ctgctggccc tccgcctgcg cgaaccctcc cccggaaggt
     4861 acgaatagac gctaaacgcc aagacgccaa gacgccaagc accacgccaa gagtacgaaa
     4921 acgcaaagac gcaaaggtcg caaagaacgc gcaaaggtta aggattgtag cgttctaagc
     4981 aaaaggagca gggtacccat gtcgtaccct gctcctttta ctactctcta taccctaata
     5041 acct
//
LOCUS       DASRWZ010000748         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_334059, whole genome shotgun sequence.
ACCESSION   DASRWZ010000748 DASRWZ010000000
VERSION     DASRWZ010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334059"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VFH60_11890"
     CDS             complement(<1..1172)
                     /locus_tag="VFH60_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6314527.1"
                     /translation="MQRSTHRLDRLVLLIGAATMLLVSTVRADAHGAAGATQEQGRNL
                     GLGNIPVAGRFLQEWSRQGNEQASVYVNGWPITARRPEISLEDGKSYEVQWFERARYE
                     AHPEHKAPYDVLLGRLGATLVEGRGRVDPNTKAVRNPADLPFRGIERPPDADRKSKVW
                     FRETGHAVSGKFLEQWNRYGGLAQFGYPLSEQFEEVSPTDGKTYTVQYFERNRFELHP
                     GNPAPYDVELGLLGVQQHLLTPVPADQLPIAPPKGVTSSKDTMVQASLQEPTSLVGFE
                     ENTLVAARHLDAITFQDRLVHADDKGSWFPLAAWYVPSFENGGSYFVGAGEDRHLVTK
                     YKLRPGIKWSDGKEVTSNDAVFAYSLLLRDPHAVATERLTKLASVDNPDKYTLIYN"
     gene            1351..2475
                     /locus_tag="VFH60_11895"
     CDS             1351..2475
                     /locus_tag="VFH60_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET6314528.1"
                     /translation="MMSKQRARDLGIPFDGTPGPLNAITDIAGLEVGHTTLISGEGEL
                     KVGRGPVRTGDTAILPRGKQSVVPCFAGWFSLNGNGEMTGTTWVEESGFLEGPVMITN
                     THSVGVVRDAVIAWYVREMQKPGLNIMGDDFWSLPVVGETYDGYLNDINGFHVKEEHV
                     FGALDGASATNVEEGNVGGGTGMICYQFKGGIGTASRRLEAEQGGYTLGVLAQCNFGL
                     RRQLLIAGVPVGREMAHDLPGPASAGNPTGDVGSVIVVAATDAPLLPHQLKRVARRIS
                     MGLARTGSVAGNSSGDIFVAFSTADPGALDARTSNVEMLSNNHISPLFEATVQATEEA
                     VVNALMAAETMVGRDNHRVVALPYDRLKEVLKEYNRFQGL"
     gene            2693..2824
                     /locus_tag="VFH60_11900"
                     /pseudo
     CDS             2693..2824
                     /locus_tag="VFH60_11900"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            2850..>3611
                     /locus_tag="VFH60_11905"
     CDS             2850..>3611
                     /locus_tag="VFH60_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314529.1"
                     /translation="MFGGKKSASAVEDRNWSGLLDAIIHLDPKRFRGYIKGNKLLKAL
                     QCYLTPDDPCTSIRPTGKKRPLYVLSTDFNTGQETVWCFTRHVTPVDVREGAEADQFE
                     PAPGRQAFWEVRRDNDPQIQQFPTLAEVCRCSASIPMIFVPARAPIKCTDGTTIDRLY
                     TDGGVRDNYSLSTAVKLAGCDRVIGMFLSSLVITNKPWDGLIDLQQRTMSQMGMTIFE
                     ADQDDAQIRTTDIRTLVPYFAGQITTFDIKAMPSLY"
BASE COUNT          712 a         1092 c         1132 g          675 t
ORIGIN      
        1 cagttgtaga tgagcgtgta cttgtcagga ttgtccacgg atgccagctt tgtcagccgc
       61 tcggtggcga cggcgtgcgg gtccctcaag agcaacgagt aggcgaacac cgcatcattg
      121 gaagtgactt ccttgccgtc ggaccacttg ataccgggcc gcagcttgta cttggtgacc
      181 aggtggcggt cctcacccgc acccacgaag taggagcctc cattctcaaa tgacggcacg
      241 taccaggccg ccagcgggaa ccagctgccc ttgtcgtcgg cgtgcacgag ccggtcctga
      301 aaggtaatgg catcaagatg gcgcgccgct actagcgtat tctcctcgaa gcccaccagg
      361 cttgtaggct cctggagcga cgcttgcacc attgtgtcct tgctggaagt cacccccttc
      421 ggtggggcga taggaagttg gtcggcaggc acgggtgtaa gtaggtgctg ctgcactccg
      481 agtaggccca actccacgtc gtagggtgcc gggttgccgg ggtgtagctc gaagcggttg
      541 cgctcaaagt actgcaccgt gtaagtcttg ccgtcggtgg ggctgacctc ctcgaactgc
      601 tcgctgagag ggtacccgaa ctgcgccagg ccgccgtagc ggttccactg ttccaggaac
      661 ttgcccgata cggcgtgtcc ggtctcccgg aaccacacct tgctcttgcg gtctgcgtcc
      721 gggggccgct cgataccccg gaaaggcaag tcggcagggt tgcgtacggc tttcgtgttg
      781 gggtctactc tgccccgtcc ctccaccaag gtagcgccaa gtcgaccgag gagcacgtca
      841 taaggagctt tgtgctcggg gtgggcctcg tagcgggcgc gctcgaacca ctgcacctcg
      901 tagctcttgc cgtcctctag ggagatttcg ggcctgcgag cggtaatggg ccagccgttc
      961 acgtacacgc tagcctgctc gttgccctgc ctgctccact cctgcaagaa cctccctgcc
     1021 acgggtatgt tgccgaggcc gagattgcgg ccctgctcct gcgttgcacc agcggcaccg
     1081 tgagcgtccg cccgcaccgt agatacgagc agcatagttg ccgcgcctat gagtagcact
     1141 agacgatcca ggcggtgtgt agagcgctgc atgcttcccc tcctcgtacc tgtcgaacgg
     1201 tttactatta cacaacatcg gctaccacaa tggtttcatt ttcagtgaaa gtccgttgca
     1261 gtacgcccgc cccggcggag gtcggtccgg gggtgggcac gtagagggcg ttctggtatc
     1321 atggcctcca acactccgaa cgggaggcac atgatgagca aacagcgcgc ccgtgacctc
     1381 ggcatcccct ttgacggcac ccccggcccg ctcaatgcta ttaccgacat cgcgggttta
     1441 gaagtggggc atacgaccct catctccggc gagggagagt tgaaggtagg tagggggccg
     1501 gtgcgcaccg gcgacacggc gatactgcca cgtggcaagc agtcggtcgt accctgtttc
     1561 gcgggctggt tctcgctcaa cggcaacggt gagatgaccg gcaccacctg ggtagaggaa
     1621 tcgggcttcc tcgaaggccc ggtgatgatc accaacacgc acagcgtggg cgtggtgcgg
     1681 gacgcggtga tcgcctggta cgtgcgcgag atgcagaagc cgggcctcaa tatcatgggg
     1741 gacgacttct ggtcgctgcc cgtggtgggc gagacctacg acggctacct gaacgacatc
     1801 aacggcttcc acgtgaagga ggagcacgtc ttcggcgcgc tcgacggggc cagcgccact
     1861 aacgtagagg aaggcaacgt gggcggcggc accggcatga tttgctacca gttcaagggc
     1921 ggcataggca cggcctcgag gcggttggag gccgaacagg gtggttacac gctcggcgtg
     1981 ctggcgcagt gcaactttgg tctgcggcgg cagctcctca tcgcaggggt gcctgtaggc
     2041 cgcgaaatgg cgcacgattt gcccggcccc gcctctgcag gcaacccaac gggcgacgta
     2101 ggctcggtca tagtggtggc cgcgacggac gcgcctttgc tgccgcacca gctaaagcgg
     2161 gtggcgcgcc gcatcagcat gggcctggcg aggacgggca gtgtggcggg caatagctcc
     2221 ggcgacatct ttgtcgcctt ctccactgcc gatcccggcg ctctggatgc gcgcacgagc
     2281 aacgtggaga tgcttagcaa caaccacatc agcccgctgt tcgaggcgac tgtgcaggcc
     2341 acggaagaag cggtcgtcaa cgcgctcatg gccgccgaga cgatggttgg gcgcgacaac
     2401 caccgcgtag tcgcactgcc ttacgaccgg ttgaaggagg tgctgaaaga gtacaaccgc
     2461 ttccagggac tatgatggcc ggcccaggcg aggctatacc tctacaccga ttctcttgac
     2521 ttactgctct aataacactt gactagaccg gcacctggat tgtataacca tcgctgagtc
     2581 gggagagttg gcattagcat ggtagtctca ccatgaagga ggggcaggtc ttatggcgaa
     2641 gctaggactg gtgttgagcg gcgggagcat acgaggggta gcccatatcg gcatgctcaa
     2701 ggcgctcgaa gagtacgaag agtacgactt gctgaaagat atcgagattg tagttggcac
     2761 ctcggcaggg tcgattgtgg cctccatgtt cgctgtcggc tacacccccc cacgaaatgt
     2821 ggaaaatctg ggacgaggac gtttggcgtt tgttcggggg caaaaagagc gcgagcgccg
     2881 tcgaagaccg caactggagc gggctgctgg acgctatcat acacctcgac ccgaagcgct
     2941 tccgtgggta cataaaaggc aacaaactgc tgaaagccct ccaatgctac ctcaccccgg
     3001 acgacccttg caccagcatt cgccccaccg gaaagaaacg ccccctctac gtcctgagca
     3061 ccgacttcaa cacgggccag gagacggtct ggtgctttac ccgccatgtc actccggtag
     3121 atgtgagaga gggagcggag gcggaccaat ttgagcctgc tcccggcagg caggctttct
     3181 gggaggtacg ccgggacaat gacccgcaaa tacagcagtt cccaaccctg gctgaggtct
     3241 gccggtgcag cgccagcatt ccgatgatct tcgtaccggc ccgcgccccc attaaatgca
     3301 ccgacggaac gacgatagac cgcctctaca ccgacggcgg cgtccgggat aactacagcc
     3361 tgagcaccgc cgtgaagctc gccgggtgcg accgggtaat aggcatgttc ctcagctcgc
     3421 tggtcattac caataagccg tgggacgggc tgatcgacct tcaacagcgc acgatgagcc
     3481 agatgggcat gaccattttt gaagccgacc aggacgacgc tcaaatccgc acgaccgaca
     3541 tcaggaccct ggtgccatat ttcgccggtc agatcaccac attcgacata aaggccatgc
     3601 cgtcgctcta c
//
LOCUS       DASRWZ010000749         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1088181, whole genome shotgun sequence.
ACCESSION   DASRWZ010000749 DASRWZ010000000
VERSION     DASRWZ010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088181"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /gene="asd"
                     /locus_tag="VFH60_11910"
     CDS             <1..832
                     /gene="asd"
                     /locus_tag="VFH60_11910"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET6314530.1"
                     /translation="FSALPSEAAREIEPRLAQQGIGVVSNASTYRMQEDVPLMVPEVN
                     PDHLRLIEVQRRQRGWPGFIVTNPNCSTISLTLALAPLHWAFGLDSAYVTTLQAISGA
                     GFNGVSALDMVDNVVPYIGSEEDKIETEPLKLLGTLAGEAGNGLHIEPPDGLRLSAQC
                     TRVPTTDGHLEMVSVKLNSLVTHQDIVDAWESWRPAPQQLDLPSAPARPVVVRTEPDR
                     PQPRFDRDTEGGMATVVGRLRPCPLLDWKFALLGHNLVRGAAGGVLLIAELLKSEGYV
                     "
     gene            899..2041
                     /locus_tag="VFH60_11915"
     CDS             899..2041
                     /locus_tag="VFH60_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET6314531.1"
                     /translation="MAANTTPSPQNTLQIVQLGLGGVGQALVRHYIDLAGSYPWLRYA
                     GIADRSGLLLLEGGWTLDDLQSVLSSKAGGSGLAQFAGSFGARANFTPASEESAMPPL
                     DALFSSGERAAAGEFVVVDVTAERKAYDALVQARQHGAHLVLCNKWALAEDIERYDAL
                     VHGGSGRFLYETTVGAALPVINVLDSLLRTGDEVTAIDAAISGTLGYISTEIQTGVPF
                     SAALAGAQSLGYTEPDPRDDLGGVDARRKALILARRLGMRLNMADIQVESLVPPGLDD
                     ISLTAFWQEIPLADMAYVNRVAEARARGNVLRYLARIDASGASVALREVPLDSLAGGL
                     SGTESLFVFHTRRYGEQPLAVRGRGAGAEITASGVLGDILRLGGVL"
     gene            2047..>2583
                     /locus_tag="VFH60_11920"
     CDS             2047..>2583
                     /locus_tag="VFH60_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET6314532.1"
                     /translation="MKFGGTSVGSAERIKGAAQIVASYAGTERVVVVVSAVSGVTDKL
                     LAAARKAAAREHFEALELVLEIRSAHASIIEGLGLCDSEAALLRGDVQLIVGELQNFV
                     QSISILGEVTLRTLDYVAGMGERLSSRLVSAALRNIECASEAVEATRFVVTDATFGNA
                     YPLMEPTRARAAEVLGPLL"
BASE COUNT          466 a          795 c          863 g          459 t
ORIGIN      
        1 cttcagcgcg ttgccctccg aggcggccag ggaaatagag ccccgacttg ctcaacaggg
       61 tataggcgtg gtgtcgaacg ccagtacgta ccgtatgcag gaggacgtgc cgctcatggt
      121 gccggaggtg aacccggacc acctgcgctt gattgaagtg cagcggcggc agcgcggctg
      181 gcccggcttc atcgtgacca accccaactg ctcgaccatc agcctcacgt tggccctggc
      241 cccgctccac tgggcgtttg gcctcgatag cgcgtatgtg accaccctgc aagccatctc
      301 cggcgcgggc ttcaatggag tcagcgccct ggacatggtt gacaacgtcg taccttacat
      361 aggcagtgag gaagacaaga tagagaccga gccgctcaag ctgctgggta ctctcgcggg
      421 cgaagcgggc aacgggctgc acatcgagcc gcccgacggc ctgcgcctga gcgcccagtg
      481 cacccgcgtg cccaccaccg acgggcacct ggagatggtg tcggtcaagc tgaatagcct
      541 cgtgacgcac caggacatag tcgatgcctg ggaaagttgg cgacccgcac cgcagcagtt
      601 ggacctccca agcgcccccg cccgccccgt ggtggtgcgt accgagcctg accgccccca
      661 gccccgcttc gaccgcgaca cggagggcgg tatggcaacc gtagtcggca ggctgcgccc
      721 ctgtcccctg ctggactgga agttcgcgct gctgggacac aacctcgtgc gaggcgctgc
      781 gggtggcgtg ctactcatcg cggaactgct caaatcggag ggctacgttt agccgcaccg
      841 gagcttgcgg ctaagtatcg ttatccgtag tacccaaaga cggctagaaa gtacattcat
      901 ggcagctaac accacaccaa gtccgcagaa cacgttacaa atcgttcaac ttggcctggg
      961 cggggtaggg caggcgctgg tgcggcacta catcgacctt gctggcagtt acccctggct
     1021 ccgctacgcg ggcatagccg accgttccgg cctcttgctg ctggaagggg gctggacgct
     1081 ggatgacttg caatcggtgc tgagctcaaa ggccgggggc agtgggcttg cgcagtttgc
     1141 aggctcgttc ggcgcccgcg ccaacttcac ccccgcctcc gaagagtccg ccatgcctcc
     1201 gctggacgcc ctgttctcat ccggtgagcg ggcggctgcc ggtgagttcg ttgtcgtgga
     1261 cgtgaccgca gagcgcaaag cctacgacgc actggtgcag gcaaggcaac atggcgcgca
     1321 cctcgtgttg tgcaataagt gggcgctggc ggaagatata gaacgctacg atgccctcgt
     1381 gcacggcggc agcgggcgct tcctctacga aaccactgtg ggcgcggccc tgcccgtcat
     1441 caatgtgctc gacagcctgc tgcgcaccgg cgacgaggtg accgccatcg acgcagccat
     1501 cagcggtacc ctgggctaca tatccactga gatacagacg ggcgttccgt tcagcgcagc
     1561 gctggcgggt gcgcagtcgc tgggctacac cgaacccgac ccacgggatg acctgggcgg
     1621 cgtggacgcc cgccgtaaag cactcatact ggcacgtagg ctcggtatgc ggctcaacat
     1681 ggccgatata caggtggagt cgttggtgcc gccaggcttg gacgacatat cccttacagc
     1741 tttctggcag gaaatacctc tggccgacat ggcatatgta aatagagttg ccgaggcacg
     1801 agccagaggc aacgtattac gctacctggc ccgcatagac gcttccggcg cctcggtggc
     1861 cctgcgcgag gtgcccctcg acagcttggc gggcggcttg agcggtacgg agagcctgtt
     1921 cgtgttccac acgcgccggt acggtgagca acctctagca gtacgtggga ggggagcggg
     1981 ggccgagatt accgcttcgg gcgtgctggg cgacatcctg agactgggag gtgtgctgtg
     2041 atagtgatga agttcggcgg cacctcggtc ggcagcgcgg agcggatcaa gggtgcggct
     2101 caaattgtgg caagctacgc aggcaccgaa agggtagtgg tggttgtgtc cgccgtgtcg
     2161 ggtgtgaccg acaaactgct tgccgccgcc cgcaaagccg ctgccaggga gcacttcgag
     2221 gcgctggagc ttgtgctgga gatacgtagc gcccacgcct ccatcatcga gggattgggg
     2281 ttgtgcgatt cggaggctgc cttgctacgg ggtgacgtgc agctcatagt gggcgagttg
     2341 cagaacttcg tgcagagcat ctctatcctg ggcgaagtga cgctccgcac tctggactac
     2401 gtggccggta tgggcgagcg gctgtccagc aggctagtat cggctgcgtt gcggaacata
     2461 gagtgcgcgt ccgaggcggt ggaggcaacc cgcttcgttg tgaccgatgc gaccttcggc
     2521 aacgcctacc cgctcatgga gcctacgcgt gcgcgggcgg cggaggtgct ggggccgctc
     2581 ctc
//
LOCUS       DASRWZ010000750         3892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_745944, whole genome shotgun sequence.
ACCESSION   DASRWZ010000750 DASRWZ010000000
VERSION     DASRWZ010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745944"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1870
                     /locus_tag="VFH60_11925"
     CDS             242..1870
                     /locus_tag="VFH60_11925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6314533.1"
                     /translation="MSATQQEATTGVEPRGWRLAGKQAVLNWPLTALAAWAVLLLGGA
                     LRLYHLDALSYWLDEGNTVYDTRREWDVVLGFHGRYTEHPPLYHAIVKLLAVVFPDNI
                     AARVFSFICGTLTVVVLYFLVAALLNRRAAIVASVALAVSPVHVWYSQEARMYAPTMF
                     AVAASYLMLVLFYKGPQVKWAVLYGLSVLLAVYMSYSSFYALAPQVALVLLLVWKHRR
                     RAVPVLVAGACAVVLYLPWLPQMLASAEQWRGRRDSYLAPTPYRVSTSLLSVIGAGGD
                     ASTTGVPHVGYYWGWKYTAWDLGFILPRTALDPILLVRVIILLGMGAVVVLAALALWR
                     RSRLGVAVALLLLGTIPAAILASQVSAGYAERTILTATMGWAMIVGGAAYASTLGGWP
                     KWARAASLLAVVFAIGIAGLSLRALYNGGIKQDWRGLAAGTAEAARSGRQVIVYPTLS
                     ETLLDIYQPQLKQGKFIRVDDYGSMPDLSQLPGGKPDTVLFSYFNSVNIGTVRSQLEA
                     QGYERRDQRNWVGELYLDTYALPGSAGLPRGDLP"
     gene            2553..>3892
                     /locus_tag="VFH60_11930"
     CDS             2553..>3892
                     /locus_tag="VFH60_11930"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET6314534.1"
                     /translation="MSTIETSAGPVAKESGVARYFEFARYNVTFASEVMAGATTFLVM
                     AYIAFVNPGILSSVPDATGFSLDRGATISATCWVASFLCILMGAFARRPFAMAPGMGL
                     NAVVTFQFVAAQRMTWPQAMGIVVLEGIIITILVLTKFRQAILDAVPMDLKRAIAAGI
                     GLFILYIGLNQGGFVAGNPIYAVDPTVPPVQLGNFGTVTILVSVFGVLLTAWLHVRKN
                     RAAILIGILGATAFAMVLNYITMALGNPALYPNNIAQLPGFPIAVPTLPYFFGLDLGA
                     FSSQGPLTSSLDTFSLMLSDFFDTMGTFVGVALLAGFLTKDGKLPDVEKPLLVDSVGP
                     IVGGAFGASSATTYIESGAGVSAGGRTGLVAITVGVIFLFVPFLTPVIGIVPIHATSA
                     ALIMVGFFMLQTLRDVDWGNFREAFPVLITMIAMPLTYSITNGIGFGFILFVLMS"
BASE COUNT          657 a         1196 c         1223 g          816 t
ORIGIN      
        1 cacgtgttcg agtcacgtcc tcggcaccac aattcaggca caaaaatagc tcccttgtcg
       61 cacacagggg agcttttttg taggttcttt cccgcctcgc ataatatcgt gttagaatta
      121 gctcgttacc aggggtgccg gtcctaattt ggctcaagaa gcgcccgcgt ttactctccc
      181 tggtgtctaa agtagctatt ctttactggt gaggcaaggg tccacggagt aggaggagga
      241 tttgtcagca acgcaacagg aggccacgac cggggttgag ccgcgcggct ggcggctggc
      301 gggcaagcag gccgtgctca actggccgct gactgccttg gccgcctggg cagtgctctt
      361 gctgggtggg gccttgcgcc tctaccacct cgacgcgctc agctactggc tcgatgaagg
      421 caacacggtg tacgacaccc gccgcgagtg ggacgtcgtg ctcggcttcc acggcaggta
      481 caccgagcac ccgcccttgt accatgctat cgtcaagctc ctggcggtgg tcttccccga
      541 taacatcgcg gcccgtgtgt tcagcttcat ttgcgggacg ctcacggtgg tcgtgctcta
      601 cttcctggtg gctgctctgc tcaaccgcag ggcggctatc gtggcaagcg tggcgctcgc
      661 ggtatcgccg gtgcacgtct ggtactcgca agaggcgagg atgtacgcgc ccacgatgtt
      721 tgccgtggct gcgtcctacc tgatgctcgt cctgttctac aagggtccgc aggtcaagtg
      781 ggcggtcttg tacgggctgt cggtgctttt ggccgtctac atgagctaca gctcctttta
      841 cgccctggcc ccgcaggtgg cgctcgtgct gcttcttgta tggaagcacc ggcgtcgggc
      901 ggtcccggtg ctggtggcgg gagcgtgcgc cgtggtgctt tacttgccct ggctcccgca
      961 gatgctcgct tctgccgagc agtggcgcgg ccggagggac agctacctgg caccgacacc
     1021 ttaccgtgtc agcacctcgc tcctgagcgt gattggcgcg ggcggcgacg cttcgaccac
     1081 gggtgtgccc cacgtcggct actactgggg ctggaagtac accgcctggg acctgggctt
     1141 catattgccc aggaccgccc tcgatcccat tctgctggta cgcgtgataa tccttctcgg
     1201 catgggggcc gtggtggtcc ttgccgcgtt ggccttgtgg cgtcgcagcc gcctgggggt
     1261 ggccgtggcg ttgctgttgc tggggactat tccggcggca attctcgcca gccaggtcag
     1321 cgcggggtac gccgagcgca ccattttgac cgcgacgatg ggctgggcga tgatcgtcgg
     1381 tggcgccgcc tacgcttcga ccctgggtgg gtggcctaag tgggcgcgtg cggcgagcct
     1441 gctcgcggtg gtgtttgcta tcggtatcgc ggggctatcg ctgcgggccc tgtacaacgg
     1501 tggcatcaag caagactggc ggggactggc ggcggggacg gcggaggcgg cgaggtcggg
     1561 gcggcaggtt atcgtatacc ctacgctgtc ggagaccttg ctggacatat accagcccca
     1621 actgaagcag ggcaagttca taagggtgga tgactacggc agtatgcccg acctgtcgca
     1681 actaccgggg gggaagcccg ataccgtcct gttctcgtac ttcaactcgg tcaacatcgg
     1741 cacggtcagg tcgcaactgg aagcgcaggg ctacgagcgg cgcgaccaaa ggaactgggt
     1801 gggcgagctt tacctggaca cctacgccct gcctgggagc gcgggcttgc ctcggggcga
     1861 cctgccataa ccgacgatta gaaatacccg tttgctccta tcggcaccgg agctcagggt
     1921 gccctgtggc taaagccacg ggcttagaag aggcaaagtc ccctgcgggg actgaggaac
     1981 gtcatgtagt ctccgcagga gacttcgtct ctggtaagcc cgccaattca ttggcagggc
     2041 acccccgcag gccgtccatg cggggtcaca gggcttccct actaagtagg gaagcatctc
     2101 cttggcagag ccgatatcaa ggtattgtca atctcctcct ttgatgaaag gtcaggaatg
     2161 tgccgcgcaa caaattggta agcatttcca cagaatccac ctccccaaag tctcgttgac
     2221 tctcgattcc ccatcctata tactgctcgg ctatcagggg tagattaagc ggtctgccgc
     2281 tgcaacgagc cgcacaaata cccatacggc gaggcacaag ctacgagggc caaaccccgt
     2341 agcccggcca caatttacct gttcccggcg ctgttaccca cgtaggtgtg ggtatgcggt
     2401 agggagcatt gccgtattcc gcgatgggaa aggaggaggc gcgagtccgg cggaggagga
     2461 ctagcaaacg gtacgattcc ggtggtccgc agtggccgga gctgcacggc gagcagagtt
     2521 gtcggagcgc acatgtagga ggaggagcgc ggatgtcaac tatcgaaacg agcgcaggcc
     2581 ccgtagccaa agagtcgggc gttgcgcgct actttgagtt tgcacggtac aacgtcacct
     2641 tcgccagcga ggtcatggcg ggggcgacca cgttcctggt catggcgtac atcgcttttg
     2701 tgaacccagg catcctgagc agtgtccccg atgcgacggg cttcagtctg gacagagggg
     2761 cgaccatctc cgccacgtgc tgggtggcaa gtttcctttg catcctgatg ggtgcgttcg
     2821 cccgcaggcc gttcgcaatg gcccccggca tgggcctcaa cgcggtggtc acgttccagt
     2881 tcgtggccgc tcaacgcatg acgtggccgc aggcgatggg catagtggtg ctcgaaggca
     2941 tcattatcac catattggtg ctcaccaagt tccgccaggc gatactggac gccgtgccga
     3001 tggacctcaa gcgggccatc gccgccggta tcggcctctt catcctctac atcggcctca
     3061 accagggcgg tttcgtggca ggcaacccca tctacgccgt ggaccccacg gtgccacccg
     3121 tacagcttgg caacttcggc accgtcacca tactcgtctc ggtgttcggt gtgctgctga
     3181 ctgcctggct gcacgtccgc aagaaccggg cggccatcct gataggcata ctcggagcaa
     3241 ccgcgttcgc catggtgctc aactacatta cgatggccct tggcaacccc gcgctgtacc
     3301 cgaacaatat agcccaactg cctggctttc cgattgccgt gccgactttg ccctacttct
     3361 tcggcctcga cctgggcgcg tttagctcgc aggggccgct cacaagctcg ctcgatacgt
     3421 tctcccttat gctcagcgac ttcttcgaca cgatgggcac cttcgtgggt gtggccctcc
     3481 tcgccgggtt cctcaccaag gacggcaagc tgcccgacgt tgagaagccg ttgctggtcg
     3541 actcggtcgg cccgatagtg ggcggggcgt tcggtgcgag cagcgccacg acctacatcg
     3601 agtctggcgc gggcgtgtcg gctggcgggc gcacggggct ggtggctatc accgtgggcg
     3661 tgatattcct ctttgtgccc ttcctcaccc ccgtgatcgg catcgtgccc atacatgcca
     3721 cttccgccgc cctcatcatg gtcggcttct tcatgctgca aacgctcagg gacgtggact
     3781 ggggcaactt ccgcgaggcc ttccctgtgc tcatcacgat gatagccatg cccctcacgt
     3841 atagcatcac taacggcatc ggcttcggct tcatcctctt cgtgctcatg tc
//
LOCUS       DASRWZ010000751         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1361898, whole genome shotgun sequence.
ACCESSION   DASRWZ010000751 DASRWZ010000000
VERSION     DASRWZ010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361898"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1273)
                     /locus_tag="VFH60_11935"
     CDS             complement(119..1273)
                     /locus_tag="VFH60_11935"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HET6314535.1"
                     /translation="MTSLTWHQVCAWRLSQHALSPRLQRRDFLDAVTRTGGIQAQVLS
                     AAELALWARVDGLAREHVQSALWQDRTLVKTWAMRGTLHLMRAADLPLYVAARSADET
                     HNWLKYFTYYGITEAQYEAFMAAVPQVLRSEPLTREQLASAVARHIGAPELERLLLAS
                     SWGSLWKPSAWRGDLCFGPNQGRNVTFVNPRHWLGPDAKLNGPSLEPYTALQETARRY
                     LRAYGPATPEDFARWWDGSAGIVPAKKLFRSIEDELEAVDVEGWHALALRATLEPMQE
                     LEAAAPGVVRLLPLFDAYTLGLGRNIEPVLPRAYKSRVFRPQGWISAVILVDGRMKGV
                     WEYETRPSQTTVKVSMFASPAASVHEGIEAEAERLNAFLNTKVVLKFEDP"
     gene            complement(1321..2235)
                     /locus_tag="VFH60_11940"
     CDS             complement(1321..2235)
                     /locus_tag="VFH60_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET6314536.1"
                     /translation="MKLGLLTAAFPDMPLDQLAGWAAGNGFQMLEVACWPQSTASRRY
                     AGVTHIDVTELTPSKAADILGLLDRHGLQISSLAYYPNPLHPDLDHRATVIDHLKRVI
                     AAAEMLKVPIVGTFVGRDQHRTVEDNFGEFEKVWPPIVSFANDHGVKIAIENCPMIFS
                     NDEWPGGTNLATTPAIWRRMWEIIPDPGFGLNLDPSHLVWQMIDYTRVVYEFGSRILH
                     VHAKDMRIDREGLYQRGVLSAGMGWEIPRLPGLGEVRWDQFISALYAVGYDYVISIEH
                     EDRAFEGTEDLVKRGFLISRDVLKPYIY"
     gene            complement(2256..>2587)
                     /locus_tag="VFH60_11945"
     CDS             complement(2256..>2587)
                     /locus_tag="VFH60_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6314537.1"
                     /translation="SGIAWDGERPNELWIGHRDRPNEVLLKDPALLSPEARQFAFYPG
                     GHAEGFPDTFKSLYQVVYRYLDAGDFEAPPDFPTFEDGHRALRVDEAVWQSAQEDRWV
                     SLSEIGE"
BASE COUNT          459 a          801 c          821 g          506 t
ORIGIN      
        1 tacgactgcc acctgattcc tcgctacgct cggaatgaca agggccactt tttggtgctt
       61 gtatttcata ctgtcaagct tgagataggt tcgggtcaat ggctgaacat tgggaagatc
      121 aagggtcctc aaacttcagc actaccttag tattcaagaa ggcattcagc cgctccgcct
      181 cggcttcaat accctcgtgg accgaagccg ccggtgatgc gaacatgctg actttcacgg
      241 ttgtttgcga gggccgcgtc tcgtattccc agacgccttt catacgcccg tctacgagta
      301 tgacagccga gatccaacct tgcggtcgaa agacgcggct tttgtacgca cggggtagta
      361 ccggctctat gttgcgtcca agccccaacg tgtaggcgtc gaacaatggc agcaatcgca
      421 caacccctgg cgctgctgct tctagctctt gcattggctc aagagtagca cgcagggcta
      481 gcgcatgcca gccctccaca tcgacggctt ccagctcgtc ctctatggag cggaagagct
      541 ttttggcggg gacgatcccg gcggagccgt cccaccagcg ggcgaagtct tcaggcgtgg
      601 cggggccgta agcccgtaga tagcggcggg cagtctcttg cagcgcggtg taaggctcta
      661 gggacggacc gttgagtttc gcatccgggc caagccagtg gcgtggattg acgaaggtga
      721 cgttgcggcc ctggtttggc ccgaagcaga ggtcgccgcg ccaagccgag ggcttccata
      781 gcgaacccca gcttgaggcg agcaggagtc tctccagctc cggcgcaccg atgtgcctgg
      841 ctaccgcact tgccagttgc tcgcgagtga gcggttcgct gcgcaggacc tgcgggacgg
      901 cggccatgaa cgcctcgtac tgcgcctccg ttatgccata gtaggtgaaa tacttcagcc
      961 agttgtgagt ctcatccgcg ctacgagcgg ctacgtacaa gggcaaatcg gcggcgcgca
     1021 tgaggtgtaa cgtgccacgc atggcccagg tcttgacgag ggtgcggtcc tgccagagcg
     1081 cggattgcac gtgctcgcga gcaaggccgt ccacccgcgc ccacaacgcc agctcggctg
     1141 ccgacagcac ttgcgcctgg atgccgccgg tgcgcgtcac ggcatccagg aagtcccggc
     1201 gttgcaggcg cggtgacagg gcatgttgtg acaagcgcca ggcgcaaacc tggtgccacg
     1261 tcaacgaagt catagccacc cccttgtcct ttcttccatg agggtgtccg gctccagcgc
     1321 ttaatagatg taaggcttca gcacgtcgcg cgagatgagg aagccccgct tgaccaaatc
     1381 ttcggtgccc tcgaaggcgc ggtcttcgtg ctcgatgctg atcacgtagt cgtagcccac
     1441 cgcgtacagg gcgctgatga actggtccca cctgacttcg cccaggcccg gcaggcgggg
     1501 tatctcccag cccatgcccg ccgacagcac gccccgctgg tacagcccct cgcggtcgat
     1561 gcgcatgtcc ttggcgtgca cgtgcaggat gcggctgccg aactcgtaca ccacgcgcgt
     1621 gtagtcgatc atctgccaga ccaggtgcga agggtcgagg ttgaggccga agcccgggtc
     1681 ggggatgatc tcccacatgc gccgccagat ggcgggggtg gtcgccaggt tggtgccgcc
     1741 gggccactca tcgttggaga agatcatcgg gcagttctca atcgcgatct tcacaccgtg
     1801 gtcgttggcg aaactgacaa tgggcggcca gaccttctcg aactcgccaa agttgtcctc
     1861 cacggtcctg tgctggtcgc gccccacgaa agtgcccacg atgggtactt tcaacatctc
     1921 ggcagccgcg attacccgct tgaggtggtc tatgaccgtg gccctgtgat ccaggtcggg
     1981 gtgcagggga ttggggtagt aggcgaggga ggagatttgc aggccgtggc ggtccagcag
     2041 ccccagaatg tctgcggcct tggagggtgt cagttccgtc acgtctatgt gagtgacccc
     2101 agcgtagcgg cgggaggctg tgctctgcgg ccagcaggcg acctcaagca tctggaagcc
     2161 gttaccggcc gcccagcccg ccagttggtc cagcggcata tcggggaacg cggcggtcag
     2221 cagtcctagt ttcatggtgc tcctctcagt tggtattatt cgccaatctc gctcaggctc
     2281 acccagcggt cttcctgggc gctctgccac actgcttcgt ccaccctcag cgcccggtgc
     2341 ccgtcctcga acgtggggaa gtcgggcggc gcctcgaagt caccggcgtc caggtagcgg
     2401 tagacgacct ggtagaggct cttgaaagtg tcggggaagc cctcggcatg ccctcccggg
     2461 tagaaggcga actgccgtgc ttcgggcgag agcaaggcgg ggtctttcag cagcacctcg
     2521 ttgggacggt cgcgatgtcc tatccacagc tcgttggggc gctcgccgtc ccacgcgatg
     2581 cccgacc
//
LOCUS       DASRWZ010000752         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_540537, whole genome shotgun sequence.
ACCESSION   DASRWZ010000752 DASRWZ010000000
VERSION     DASRWZ010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540537"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1759)
                     /locus_tag="VFH60_11950"
     CDS             complement(<1..1759)
                     /locus_tag="VFH60_11950"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6314538.1"
                     /translation="MLICPLCGKYNSDDKRNCEACGAFLGQAERVAAPAPAVPAATNG
                     SSGPEGAFSGPMCPVCRRPNRATSAFCAHCGHRLRSNSADPVYAMPHVAAPPSVAGGF
                     PSAPAASVAPDVTGNLPTGTVLKRQYRILRKVAQGGMGAVYESADLQGPTGNRRAIKE
                     ISPAALPPGERTQAVADFRREAQMLARLQHSNLVKVVETFEELGKHFLVMEFVPGRTL
                     LNVLDLAGGPLAEERVMVWARQMLDVIQYLHSQDPPIIYRDLKPANIMLVEGTELIKL
                     IDFGIARLHKAGKTSDTEAFGTAGYAPPEQYGKGQTDQRSDIYAFAATLHHLLTNRDP
                     SLNPFNWLSIRRYNPGVSVRVDTALMQALNLDPQRRFQSVEAFAQALGLEGTFAPRSR
                     PLQEPPAPTPLPHVPTRSKANGQSKKKAQGTGLADALPVGPLPQPIPPPRSEPAVAAM
                     PAIPLAASIAAATQFAPAPAAPAVPATPTNEATSNGKVAQSDTATATAAPPTPDIVAP
                     STIMAPPRWEPEARTEVVEATTPAPLPAQPKSQPAEVRVPGRNGSAGGSTGASALVIS
                     DRLVDLGETRWNRGAVKRIQ"
     gene            complement(1982..>2620)
                     /locus_tag="VFH60_11955"
     CDS             complement(1982..>2620)
                     /locus_tag="VFH60_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6314539.1"
                     /translation="PKDIPPAFQQELAAVQSVTLRNVDIQVRLSAGVELRRAYRVRPA
                     IAAVVDARKQGRTVAVSMGDLDPANPPALLLELIVPTQPRGTFRIAGISVNHQTPAGS
                     QVAATRDVVVRYSMSKEREEPNATVMNTVEKVTAYVLQTRALNDMAAGNISAATQRLR
                     AAATRLLTVGETDLAQAVEAEAARLESSGQVSPEGAKEIRYATRKLTQRLEA"
BASE COUNT          409 a          797 c          918 g          496 t
ORIGIN      
        1 gctgtatgcg ctttaccgct ccccggttcc agcgggtctc tcccaggtca accagcctat
       61 ccgagatcac cagcgcggac gcgcctgtgc tgccgcctgc gctgccgttc cgccccggta
      121 ctctcacctc ggcgggctgc gactttggct gggcgggtag gggcgccggg gttgtcgctt
      181 ccacgacctc agtgcgtgcc tctggctccc atcgtggagg tgccattatc gtggaaggcg
      241 ctacaatgtc aggggtgggc ggtgcggcgg tagcagtcgc agtgtcgctt tgtgcgacct
      301 tgccgttcga cgtagcttcg ttcgtagggg ttgcgggtac ggcaggtgcg gcaggcgcgg
      361 gcgcaaactg cgtggcggcc gcaatgctgg cggctaaggg tatggcaggc atagcggcca
      421 cggcaggctc ggagcgcggc ggcggtatgg gctgaggcag agggccgacg ggcagcgcat
      481 cagctaaccc ggtaccctgt gctttcttct tcgactgtcc gttggccttg ctcctggtgg
      541 gcacgtgagg gagcggagtg ggggccggtg gctcctgtag gggcctggac ctgggggcga
      601 acgtaccttc cagacccagg gcctgagcga acgcctctac cgattggaag cgcctttggg
      661 ggtcgaggtt gagcgcctgc atgagcgcgg tatcgacccg cacggacacg cccgggttgt
      721 agcgacggat ggacagccag ttgaaagggt tgaggctggg gtcgcggttg gtgagcaggt
      781 ggtgcagagt ggcggcgaat gcgtaaatat cggaccgctg gtctgtctgc cccttgccat
      841 actgttccgg tggcgcataa ccggccgtgc cgaacgcctc ggtatcggag gtcttgcccg
      901 ccttgtgcag gcgcgcgatg ccgaaatcta tcagcttgat tagctcagtg ccctcaacca
      961 gcatgatgtt ggcgggcttg aggtcgcggt agatgatggg cgggtcctgg ctgtgcaggt
     1021 actggatcac gtcgagcatc tgcctggccc acaccatgac tctctcttcg gccagcggcc
     1081 cacccgcaag gtcgagcacg ttgagcaagg ttcggcccgg cacgaactcc atgaccagga
     1141 agtgcttgcc caattcctcg aacgtctcga ctaccttcac gaggttgctg tgctggaggc
     1201 gggcgagcat ctgcgcctcg cggcggaagt cggccacggc ctgagtgcgc tctccggggg
     1261 gtagggcggc ggggcttatc tccttgatgg cccggcggtt gccggtcggt ccctgcaagt
     1321 cggcgctctc gtaaacggcc cccatgccgc cctgagccac tttgcgaagg atgcggtact
     1381 ggcgcttcaa cacggtgccg gtgggtaggt tgcccgtaac atcgggggct actgaagcgg
     1441 cgggggctga agggaaaccg ccggcaactg acggaggagc cgccacgtgg ggcatggcgt
     1501 agaccggatc ggcgctgttc gaacgcaggc ggtggccgca gtgggcgcag aaagccgacg
     1561 tagcgcggtt ggggcggcgg cacacggggc acatcggccc gctgaacgcc ccctcagggc
     1621 cggacgagcc gttggtggcc gcgggcacag caggcgcagg ggccgcgact cgctcggcct
     1681 gccccaggaa cgccccgcac gcctcgcagt tacgcttatc gtcggagttg tatttgccac
     1741 acagcggaca gattagcatt acaacaacct atacgttggg gcggaggcca atgttacgcc
     1801 tggcggacga agtaccgagt gccgagtgcc gagtgccgag tgccgagtgc cgagtaccga
     1861 gtaccgagta ctgagtactg agtactgagt gcgagagtac tactaacata atatcatgtt
     1921 tcaagttcaa ctgttacgga ctaatcttac tcggcactcg gcactcggca ctcggtactc
     1981 gtcaagcttc cagtctctgc gtcagcttcc gcgtggcgta gcgtatctct tttgcaccct
     2041 ccggggagac ctggccgctg ctttccaggc gggcggcttc ggcctctact gcctgggcga
     2101 ggtccgtctc gcccaccgtg agcaggcggg tggcggcggc gcgcaggcgc tgggtggcgg
     2161 ccgagatgtt accggcggcc atgtcgttga gggcgcgggt ctgcaacacg taggccgtga
     2221 ctttctccac cgtgttcatg acggtggcgt tcggctcttc ccgctccttc gacatagagt
     2281 aacgaactac tacgtccctg gtagcggcca cctggctgcc cgcaggggtc tggtggttca
     2341 ccgagatgcc cgcgatgcgg aaggtgccgc gcggctgggt gggcacgatc aactccaaca
     2401 ggagcgcggg ggggttggca gggtccaggt cgcccatcga aacggcgaca gtgcggcctt
     2461 gctttctcgc gtccaccaca gccgcaatcg ccggccgcac cctgtacgcc cgcctcagtt
     2521 ccacgcccgc gctgagccgc acctgtatat cgacgttgcg cagcgtgacc gactgcaccg
     2581 ccgcaagctc ctgctggaag gccgggggta tatctttcgg
//
LOCUS       DASRWZ010000753         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1294809, whole genome shotgun sequence.
ACCESSION   DASRWZ010000753 DASRWZ010000000
VERSION     DASRWZ010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294809"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1193)
                     /locus_tag="VFH60_11960"
     CDS             complement(126..1193)
                     /locus_tag="VFH60_11960"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET6314540.1"
                     /translation="MTIDPKHSPAPGPAVLVEVTRGPIVESVHYGVVAAADVEGDLLA
                     WLGDPGFVSYYRSSSKPIQAVPIVESGAADHFGLTEPEIAICCGSHGGEDIHEEAVLS
                     ILHKIGLGTDALACGPHAPYDKAAARLLAEQGGPPTVLHNNCSGKHAGMLVLARFHGW
                     PVGGYESRLHPVQHAMHSVVAEFAGVAPEEMPVGVDGCGVLTFGLTIHAMAVSFARLA
                     DADYWPEPRRSAVRRITQARVAYPEYVAARQQRFDTDLMRAAGGTLVSKVGAEGVFCA
                     ALLPAEDRPAIGFALKVLDGDNSLRARNPSVTEGLRQTGMLTEEALNKLETYWVEEIR
                     NRPGDVVGVVRPAFTLAVAED"
     gene            complement(1259..1918)
                     /locus_tag="VFH60_11965"
     CDS             complement(1259..1918)
                     /locus_tag="VFH60_11965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET6314541.1"
                     /translation="MKHFGVVDPRLEDYMEGLLPSREGVLTDMEAHAEREGLGIIGPL
                     QGHFLYTLVLALKPKNILEVGTAIGYSAMWLSMAAEKVEGRVVTIERDNSRAVWAEAY
                     MRQAGLSGVCAVRRGEALVLMRGMEESFDLVFLDVLTQFDRPGTALELLDLCVRRLRP
                     GGVLLSDNALRSGQVLAPQDHSPSTQGIAAFNQAIFTHPRLTSTIIPIRDGLSMSVKG
                     F"
     gene            complement(1915..>2253)
                     /locus_tag="VFH60_11970"
     CDS             complement(1915..>2253)
                     /locus_tag="VFH60_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314542.1"
                     /translation="TPMLEAGADCIVLGCTHYPFLNAVLRRIAGEDVRLIDSSGGVAL
                     QTRRVLEARYLLRDDDHTGRLTVYTSGDPHVVAPVVLRLLGEEVAVLHESQVPPPSGA
                     GSPPPVQPAP"
BASE COUNT          395 a          687 c          750 g          421 t
ORIGIN      
        1 ggtgcaaaga ggaccagggc acaaatgcag taccctggtc ctccttactt agaacaccta
       61 ctaaaccttg gcgcgctctt ggcgttcttg gcgtcttggc ggttccgtcc ttattgcctt
      121 tggatttagt cttccgcaac agcgagggtg aaggcagggc ggaccacgcc cactacgtcg
      181 ccggggcggt tgcgtatttc ctccacccag tacgtttcca gcttgttgag ggcttcttct
      241 gtgagcatgc cggtctggcg caggccctca gtgacggagg ggttgcgggc gcgcagggaa
      301 ttgtcgccgt cgagcacttt gagggcaaag ccgatggcgg gacggtcttc cgcaggcagg
      361 agagcggcac agaagacgcc ctccgcgcca actttgctga ccagggtgcc accggcggcc
      421 cgcatgaggt cggtatcgaa acgctgctgg cgggcggcta cgtactccgg gtaggcaacc
      481 ctcgcctggg tgatgcggcg gaccgcgcta cggcgaggct ccggccagta gtcggcgtcg
      541 gcgaggcggg cgaaggatac ggccatcgcg tgaatcgtga ggccgaacgt cagtacgccg
      601 cagccgtcaa cgcccaccgg catctcctca ggggcgacgc ccgcgaactc cgcgacgacg
      661 ctgtgcatgg cgtgttgcac agggtgaagg cggctctcgt aaccgcctac gggccagccg
      721 tggaagcgag ccaggaccaa catcccggcg tgcttgccgg agcagttgtt atgcagcacg
      781 gtgggtgggc ctccctgctc ggcgagaagg cgagcggctg ccttgtcgta gggcgcgtgg
      841 gggccgcatg cgagggcgtc ggtgcccagg ccgattttgt ggaggatgct gagcacggct
      901 tcttcgtgta tgtcttcgcc gccgtgggaa ccgcagcaaa tggctatctc cggctccgtc
      961 agcccgaagt ggtctgccgc cccgctttcg acaatgggca ccgcctgtat aggcttggag
     1021 gaggagcgat agtagctcac gaatcctggg tcgccaagcc acgcaagcag gtccccctcc
     1081 acgtcggccg ccgcgaccac cccgtaatgc accgattcca caatcggccc gcgtgtgacc
     1141 tccaccaata ctgccgggcc aggcgcgggg ctatgtttgg gatcgattgt cattattcct
     1201 cctgtggcga ttttagattt tagattttgg attattagga gaatccaaaa tctaaaatct
     1261 aaaatccttt cacgctcatg ctaaggccgt cgcgtatggg gatgatggtg ctggtcagcc
     1321 tggggtgggt gaagattgct tggttgaagg cggcgatgcc ctgggtggag gggctgtggt
     1381 cctggggggc cagcacctgg ccggagcgca gggcgttatc cgacaggagc acgccacccg
     1441 gccgcaggcg gcgcacgcac aggtcgagca gctccagggc ggtgccgggc cggtcgaact
     1501 gcgtcagcac gtccaggaat accaggtcga agctttcctc catgccgcgc attagcacaa
     1561 gggcctcccc acggcgcacg gcgcacacgc cagacaaccc ggcttggcgc atgtaggcct
     1621 cggcccagac cgcacgagag ttgtcacgct caatcgtaac cacccgccct tcgacctttt
     1681 cggcggccat actgagccac atggctgaat agccaatcgc ggtcccaact tcaaggatat
     1741 ttttgggctt gagggccagc accaaagtat agaggaagtg gccctgtagc ggtccgatta
     1801 tgcccaggcc ttcgcgctcg gcgtgggctt ccatatcggt aagcacgccc tcgcgggagg
     1861 gtagcaggcc ctccatgtag tcttccaagc gcgggtctac gacgccgaag tgtttcatgg
     1921 cgctggctgg acgggagggg gactgcctgc gccgctgggt ggtggaacct gggactcgtg
     1981 caggactgcc acttcttcgc ccaggagccg cagtacgacg ggggccacta cgtgcgggtc
     2041 gccgctggta tagaccgtca gccgtccggt gtgatcgtcg tctcggagca ggtacctggc
     2101 ttcgagtacc cgccgggtct gcaaggccac gccgccgctg gagtctatca gccgcacgtc
     2161 ctccccggct atccggcgca agaccgcgtt gagaaacggg tagtgggtgc agccgagcac
     2221 gatacaatcg gcacctgcct cgagcatggg cgt
//
LOCUS       DASRWZ010000754         3311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1019873, whole genome shotgun sequence.
ACCESSION   DASRWZ010000754 DASRWZ010000000
VERSION     DASRWZ010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019873"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFH60_11975"
     CDS             complement(<1..1012)
                     /locus_tag="VFH60_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314543.1"
                     /translation="MRERLLILALLVIGALSVSGCNPGVEDAGAAPQQVDAPAVMGTP
                     EPAATATSVPPPTATPTPPRPTETPWPTQVVSQEVWFNIHDIEDKAAAIRGLQVKGDV
                     PEIYITPQQLHDYFAAYVGVGYTIEEAQQDEMIAWLMRLIKQRDVDLKEKAVEVHTEG
                     ILGFYSPDTKQLFVLGEADNMDAQTRATLAHEFTHALQDQHFGLVHLVQQYSHDSDRR
                     LAMRALIEGDATMSDLLYTYYHEEIDYSRQEKESVATNNAQEQSENEQDLEGLYVGRT
                     IYFPYIQGSQFVSELTRVGGYSTINQTFQDPPVSSEQIMHPEKYLQTPHDIPLNVAMP
                     PLT"
     gene            1260..1574
                     /locus_tag="VFH60_11980"
     CDS             1260..1574
                     /locus_tag="VFH60_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314544.1"
                     /translation="MLDQPERNISESKSVDGTQLLLLTVFLAVAQVRVYLLLRFWTGE
                     VTGAQLAYKVVPPSGSSNPLIYMVLLVVLFMALLCYGRIRWLAYALLLSIPGAFLFMT
                     VT"
     gene            1816..2319
                     /locus_tag="VFH60_11985"
     CDS             1816..2319
                     /locus_tag="VFH60_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6314545.1"
                     /translation="MRVKEFLSQRDITYTEKYVDRDRDAAIEMIRRSGQQGVPVTVIG
                     DQVIVGFDRPRLERAIAAMREAAPSSTGGGAGGRRSLGAKVADAGRYVMPGGTRVEGA
                     FVGGVNPGSPAESAGLKVGDVIVAVDGNPIGSVDALSEALTAARASSVKLSLARGTTR
                     REVTVQF"
     gene            complement(2363..3172)
                     /locus_tag="VFH60_11990"
     CDS             complement(2363..3172)
                     /locus_tag="VFH60_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HET6314546.1"
                     /translation="MRVIIISDMEGVSGIVKWQQVMGGEALYQECRKLYTEEINAAVR
                     GARAGGATEIVVMDCHGAGGPWSFNSLVPDILDPGCEYVVQEKWTGYTGALEQGCEAA
                     LFVGMHAMAGTPLGVLSHTVSSEAWHNLWFNDVLVGETGINAALCGTWDCPVLLVTGD
                     QATCDEARALLGDELKTVAVKQGLGRYTARQVPPVRARELIEEAAKQAIRERRPVPPY
                     SPSKPCEIKVEFVSPDRAEPYRNKPGVQIVAPRLLVSRADDWWSAWKQFWL"
BASE COUNT          579 a          995 c         1057 g          680 t
ORIGIN      
        1 cggtgagggg cggcatagcc acgttcaggg ggatgtcgtg gggcgtttgc aggtacttct
       61 cagggtgcat gatctgctcg ctcgacacgg gcgggtcctg gaaggtctgg tttatcgtgc
      121 tgtagccgcc cacccgcgtc agctcggaca cgaactggct gccctggatg taagggaagt
      181 agatcgtccg cccgacgtaa agcccttcca ggtcttgctc attttccgac tgctcctgcg
      241 cgttgttcgt ggcgacgctc tctttctctt gcctggagta atctatctcc tcgtggtagt
      301 aagtgtagag gaggtcggac atcgtggcgt cgccctcgat gagagcgcgc atggcgaggc
      361 ggcggtcgct gtcgtggctg tattgctgca ccaggtgcac caggccgaaa tgctggtcct
      421 ggagggcgtg ggtgaactcg tgggccaggg tggcgcgggt ctgcgcgtcc atgttgtcgg
      481 cctcacccag cacgaatagc tgcttggtgt cggggctgta gaagccgagt atgccctcgg
      541 tgtgcacctc cacggccttt tccttcaggt ccacgtcgcg ctgctttatg aggcgcataa
      601 gccaggcgat catctcatcc tgctgggcct cctcaatcgt gtagcccacg cctacgtacg
      661 cggcgaagta gtcgtgcagt tgttgggggg tgatatatat ctccggcacg tcgcccttga
      721 cctggaggcc gcgaatggcc gccgccttgt cctctatatc gtggatgttg aaccatactt
      781 cctggctcac gacctgcgtg ggccaggggg tctcggtcgg cctgggaggc gtgggggtgg
      841 ctgtgggcgg gggcacgctc gtggccgtgg cggcaggttc gggggtgccc atgacggccg
      901 gggcatctac ttgctggggt gccgcgccgg catcctccac gccagggttg cacccggaaa
      961 cggaaagcgc cccgataacc agcagggcca atatgagcaa tcgctcgcgc atggtaaaaa
     1021 ctccactcta taaagaagga tgacgaaaac ggcagcaaca ggacggccct tcggccacat
     1081 cccatgccca atgcaataat aatcaacgcg agtgccatgc cctatgaggc gatgctccta
     1141 gcaagtccta ctaatttgtc ctataggtca tgtggcgtgt gatatggtga attgccatag
     1201 tgtcgcacgc tcgcggctgc tgctcatcaa atggcacgga ctctgcacgg gctagatgta
     1261 tgttggacca gccggagcgc aatatctcgg aaagcaagag cgtcgacggg acgcagcttc
     1321 tcctgttgac ggtctttctc gcggtcgcgc aagtccgcgt ttacttgctg ttgcggtttt
     1381 ggacgggtga ggttacaggg gcacagcttg cttataaggt tgtaccgccg tccggttcct
     1441 ccaatccgct aatctacatg gtattgcttg ttgtgctgtt catggccctt ctttgctacg
     1501 ggcgcatccg gtggctagcc tatgccctcc tgctgagcat tccaggggcc ttcctcttca
     1561 tgaccgtcac gtagacactc taaggccagg cccagtctgc aaagtgcctc ccgcgttgga
     1621 ccaaagcggc ccctatcatt ctgagcgcag cgaagaatga caggggccag ctttgtttac
     1681 ttgtttccag cactattttg gcactcctta ggactggcac ggactctgca cactgtcacg
     1741 atgagagact gcttcgcgca aggaggccaa atgcaacccg ttactattta ctcgacccgc
     1801 acctgaccct ggtgcgtgag ggtgaaggag tttctttcac agcgcgacat aacgtacacc
     1861 gagaagtacg tcgataggga cagggacgcc gcgattgaga tgatacggcg ctccggccag
     1921 caaggcgtgc ctgtaacggt gataggcgac caggtgatag tgggtttcga caggcccagg
     1981 cttgagcggg cgatagccgc catgcgcgag gcagccccct ctagcacggg cgggggtgcc
     2041 ggtggccgcc gctctctcgg cgcgaaggtg gccgatgcgg ggcggtacgt gatgccgggt
     2101 ggcacgcggg tggaaggggc gttcgtgggt ggcgtcaacc caggctcccc agccgagagc
     2161 gccgggttga aggtcggaga cgtaatagtg gcggtggacg gcaaccccat aggctcggtg
     2221 gacgcgctat ccgaggcctt gaccgccgcc agggcaagca gtgtgaagct ctcactggct
     2281 cggggcacca cacggcggga ggtgacggtg cagttctagg gggctgcggg aatgcggccc
     2341 ccggcaagta cacggggggc tgttagagcc agaactgctt ccaggcggac caccagtcgt
     2401 ctgccctcga caccagcagc ctcggcgcta ctatctgcac gcccggcttg ttgcggtaag
     2461 gctcggcgcg gtcgggcgac acgaactcca ccttgatttc acagggctta ctggggctat
     2521 aaggaggcac ggggcggcgc tctcgtatgg cctgcttagc ggcctcctcg attagctcgc
     2581 gggcgcggac gggtggtacc tggcgagccg tgtacctgcc tagtccctgt ttgaccgcga
     2641 ccgtcttcag ttcgtccccc agcagcgcgc gtgcctcgtc gcaggtggcc tggtcccccg
     2701 tgaccagcag cacggggcag tcccaggtgc cacacagcgc ggcgttgata cccgtctcgc
     2761 cgaccagcac gtcgttgaac cacaggttgt gccaggcctc gctggagacg gtatggctca
     2821 gcacgcccag gggagtaccg gccatcgcgt gcataccgac gaacagggcg gcctcacacc
     2881 cctgctccag cgcgccggtg tagccggtcc acttttcctg cactacgtac tcgcagccgg
     2941 ggtctagtat gtccggcacg agggagttga acgaccaggg gccgcccgcg ccgtggcagt
     3001 ccatcaccac gatctcggtg gccccacccg ctctcgcccc gcgcaccgcc gcgtttatct
     3061 cctccgtgta gagcttgcgg cactcctggt atagtgcctc cccgcccatc acctgctgcc
     3121 acttgacgat gcccgatacc ccttccatgt cactgatgat tattactctc atcgcatgct
     3181 ctccgcgcaa tagatttcgg aaggccagta tgcgacaatc agccacccaa gcgcaagaac
     3241 atagctagtg cgtgcagggc ggctgcacca ggtgcgaaat cgatcatagt tggaaggctt
     3301 ctggagccga t
//
LOCUS       DASRWZ010000755         3913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1226189, whole genome shotgun sequence.
ACCESSION   DASRWZ010000755 DASRWZ010000000
VERSION     DASRWZ010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226189"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1503
                     /locus_tag="VFH60_11995"
     CDS             163..1503
                     /locus_tag="VFH60_11995"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6314547.1"
                     /translation="MPALLLKATWNHDPLPALDQTSLAYVLVDINEEQATDGSQEGSA
                     LSPQSSSLNISLVLDTSGSMGGAKLQNLKQAVNWLVDHLGEHDTLAITLFDDEVHPLT
                     GSTTLHDRDALHSQIDGIREAGGTAMSKGLLVGLDEAMKGRRDGVVSRIVVLTDGQTW
                     GDAERCEQLARQAGAAGIPVTALGIGAEEDWSIELLDSLATESGGESGYIAKPEEIAG
                     AFEETLRSMKATTYQNLRLTFNPAGGVQPRAIYRVAPIISRLWPAQQAAQDGEATAVP
                     QTDGAATAIALPVGDLQPGTGQTLLYELVLPARKPGQYRLAHLLLDYEQTGGTTSKHD
                     TALDLVVTFAGGVAKGPGDPRVMNHVERATTFKLQTRALQASMVGDVAGATRNLQAVA
                     TRLLNMGETELAEEAQRQAVRVEQQGSMSAAGTKKLAYDTRKLSVNETMILREE"
     gene            1621..3819
                     /locus_tag="VFH60_12000"
     CDS             1621..3819
                     /locus_tag="VFH60_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314548.1"
                     /translation="MNGTLQREPALPADVSRGFGIAPRTVLVPMLNLGIAGDMLHLAA
                     ILAAGHKSNVPAPVPAGDSSTPPRLVPRIVVLGVVEVPADQPLTTGMDMARSYRALFD
                     FLPSEVDAGGKQVRVDRLVKVSRDVPTAVRQAVEEEHADLVLAHWKGHPRNPKRHVYG
                     HILDDLLKDPPCDLLLARPDGWRDCGRVLLPVRGGPSAEQALDVALLLAEHGDMDLAV
                     MHNVPASRAGTGPLGEGLLKKVSTGPLTEAQVAADAMETGPYIDFNDYLEAVTGEWAV
                     RVERIATVSENAVTAVLGEVQSEDIVIMGVGAFEESGGKRTSAGAATLPPTVAASTKA
                     PILLLRARERLDLDKYARKAQGRQVRNAWVDMPFEYWFVENTYHGDEFRDPEEFLKLK
                     QATGLTISVGLLTSNDARHLHSMITGLRRVLVEMHPLADQIAVVDAGSADETLEIARS
                     LGVEAYSVPEILPGEGSLHGRGESWWKSLAVLRGDIIVWLDPRATRFHPSAALSLAGP
                     LLRNANLQLVKAFALTQHDTHGAAAPRQQEAPTEGFDPLDVSWGGFVVPKRDGAWPFA
                     RRIRVQALKPADLAALTPAQFAALPPRTILQVLSPSLAGVVDPFGRDYAARREAMLSI
                     PAFTGPNLELGMLLSAASGWGSRSVAQVELRHAQPSPPPQPGLRIAIDVLHVLAHRLE
                     DPQLCATASELAARLQSELQGPTAGPGTGIEVRALNPVERPPMTTVLGTR"
BASE COUNT          706 a         1377 c         1234 g          596 t
ORIGIN      
        1 acatcccggt aaaggacgag attcttcgct acgctcagaa tgacaaaggg gaagagcatc
       61 ttgccttcct cgtatggtag tattcacgca ccaactacga taggctggat tactcgatac
      121 tcgtcactcg ttctactgcc attttcatag aggttttgct caatgcccgc actattgttg
      181 aaagccacct ggaaccacga cccgctccct gccctcgacc agaccagcct tgcctacgtt
      241 cttgttgaca taaatgaaga acaagcaact gacggctcac aagaaggctc agccctcagt
      301 cctcagtcct catcactcaa catcagccta gtgctcgaca cgtccggctc gatgggcggg
      361 gccaagctgc aaaacctcaa gcaagcagtc aactggctgg tggaccacct gggcgagcat
      421 gacaccctcg ccatcaccct gttcgacgac gaggtacacc ccctcaccgg cagcaccacc
      481 ctgcacgacc gcgatgccct ccactcgcag attgacggca tacgcgaggc gggaggcacc
      541 gctatgagca aggggctgct ggtgggcctg gacgaggcga tgaagggccg ccgggatggc
      601 gtggtgtcgc gcatcgtcgt gctcaccgac gggcagacct ggggcgacgc cgagcgttgc
      661 gagcagcttg cacggcaggc gggcgcggcg ggcatacccg tcacggccct gggcattggc
      721 gcggaggagg actggagcat agagttgctc gacagcctgg cgaccgagag cggcggcgag
      781 tcgggctaca tagccaaacc cgaggagata gccggggcct ttgaggagac gttgcgctcg
      841 atgaaggcca ccacctatca gaacctgcgc ctcaccttca acccggcggg cggcgtgcag
      901 ccacgcgcca tctatcgcgt ggcccccatc atctctcgcc tgtggcccgc ccaacaagcc
      961 gcccaggacg gcgaggcaac ggccgtgccc cagacggacg gtgcggccac tgccattgcc
     1021 ctacccgtgg gcgacttgca gccggggacc gggcagacgc tgctctacga attggtgcta
     1081 cccgcccgca aacccggcca gtaccgcctg gcgcacctgc tcctcgacta cgagcagacg
     1141 ggtggcacta cgagcaagca cgataccgcc ctcgaccttg tggtgacgtt cgccggtggc
     1201 gtggccaagg ggccgggcga cccccgcgtg atgaaccacg tagagcgcgc caccaccttc
     1261 aagctacaga cgcgggcctt gcaagccagc atggtgggcg acgtagcagg cgctacccgc
     1321 aacctccagg ccgtcgccac gcgcctgctc aacatgggcg agacggagct ggccgaggag
     1381 gcccaacgcc aggccgtgcg cgtagagcag caaggcagca tgagcgccgc cggcaccaag
     1441 aaacttgcct acgacacccg caagctgtcg gtgaatgaga cgatgatctt gcgtgaagag
     1501 tgatgtgtaa cacgtaacgc gtaacgcgta attcgtaata acatcaggca aatctcgtta
     1561 cgcgttacgt gttacgtgtt acgctttatc cccaggaact caacggcgag gacctgacct
     1621 atgaacggta cattgcagag agaacccgcg cttccggcgg acgtttcgcg cggcttcggg
     1681 atagcgccca gaacggtgct ggtgccgatg ctcaacctgg gcatcgccgg ggatatgctg
     1741 cacctggcgg ctatcctggc tgccgggcac aagtccaacg tccccgcgcc tgtgcccgcc
     1801 ggtgacagtt ccacgccccc ccgcctcgtg cctcggatcg tggtgctggg cgtggtcgag
     1861 gtgcccgccg accagcccct cacaacgggc atggacatgg cccgctccta ccgcgcgctt
     1921 ttcgatttcc tcccctccga ggtggacgcg ggcggcaagc aggtgcgcgt ggaccggctc
     1981 gtgaaagtgt cccgtgacgt gcccacggcc gtgcggcagg cggtggagga ggagcacgcc
     2041 gacctcgtgc tggcccactg gaagggccac ccccgcaacc ccaagcgcca cgtctacggc
     2101 cacatcctcg atgacctgct gaaagacccc ccctgcgacc tgctcctggc tcggcccgac
     2161 ggttggcgcg actgcgggcg ggtgctgctg cccgtgcgcg gcggcccctc cgccgaacag
     2221 gccctcgacg tggccctgct gctggccgag cacggcgaca tggacctggc agtgatgcac
     2281 aacgtgcctg cctcccgcgc cggcaccggc cccctgggcg aggggctgct caagaaggtg
     2341 agcaccggcc cgctcaccga ggcgcaagtg gccgcagacg ctatggagac cggcccctat
     2401 atcgacttca acgactacct tgaggcagtc accggcgagt gggccgtgcg ggtcgagcgc
     2461 atcgccactg taagcgagaa cgccgttacg gccgtgctcg gagaagtgca atccgaggac
     2521 atcgtaatca tgggcgtggg ggcgttcgag gagagcggcg gcaagcgcac gagcgcaggt
     2581 gccgccacac tgccccctac cgtggccgcg agcaccaaag cccctatcct gctgctccgc
     2641 gccagagaga ggctggacct ggacaagtac gcgcgtaagg cgcagggccg ccaggtgcgt
     2701 aacgcctggg tggacatgcc gttcgagtac tggtttgtcg agaacaccta ccacggcgac
     2761 gagttccgcg acccggagga gttcctgaag ctgaagcagg ccaccggcct cactatcagc
     2821 gtgggcctgc tcacctccaa cgacgcccgc cacctgcact cgatgattac gggcctgcgc
     2881 cgcgtgctgg tggagatgca cccccttgcc gaccagatag cggtggtaga cgccggttcg
     2941 gccgacgaga ccctggaaat agcccgctcc ctcggcgtgg aggcgtacag cgtgcccgaa
     3001 atactgccgg gcgagggtag cctgcacggg cgcggcgaaa gctggtggaa gagcctggcg
     3061 gtgttgcgcg gcgacatcat cgtgtggctc gacccgcgcg ctacccgctt ccacccaagc
     3121 gccgcccttt ctctcgcggg gccgctcctg cgcaacgcca acctgcaact ggtcaaggcg
     3181 ttcgccctca cgcagcacga cacgcacggc gcggccgctc ccaggcagca ggaggccccg
     3241 accgagggct tcgacccgct ggacgtaagc tggggaggct tcgtggtgcc caagcgagac
     3301 ggggcgtggc ctttcgctcg ccgcatccgc gtgcaggccc tcaagcccgc cgacctggcg
     3361 gcccttaccc ccgcgcagtt cgccgcgctg cccccgcgca ccatcttgca ggtgctgtct
     3421 ccctccctcg ccggggtggt ggaccccttt gggcgcgact acgcagcccg ccgcgaagcc
     3481 atgctctcca taccggcgtt caccggcccc aacctggaac tcgggatgtt gctgtcggcg
     3541 gcttcaggct ggggcagccg ctcggtcgcc caggtggagc ttcgccacgc ccagccctcc
     3601 ccgcctcccc agccggggct gcgcatcgcc atagacgtgc tgcatgtgct cgcccatcgc
     3661 ctggaagacc cccaactgtg cgccaccgcc tccgagcttg ccgcgcgcct ccagtccgag
     3721 ctacaaggcc ccaccgccgg acccggtacc ggcatcgaag tccgcgccct caacccggtg
     3781 gagcggcccc ccatgaccac cgtgctcggc acccgatagg acgggaccgc caggacgcca
     3841 gtgccacacg cagacgggat caaaccccct ggggtgaccg gtcgcgcggt cattcccgcg
     3901 gtagcggagc aca
//
LOCUS       DASRWZ010000756         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1294871, whole genome shotgun sequence.
ACCESSION   DASRWZ010000756 DASRWZ010000000
VERSION     DASRWZ010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294871"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFH60_12005"
     CDS             complement(<1..474)
                     /locus_tag="VFH60_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HET6314549.1"
                     /translation="MKEEARQVTLTSLSVSEALENILSYFSPLPAVGVPLMEALDTVL
                     AEEVRSDMDNPPFDNSSMDGYAVRAEDTVGAGDDSPVSLRVTGHMPAGAFPQPGDSVE
                     PGTAFRIMTGAPVPPGADAVIRFEDTSEGRALDNARLLPQDARAQAGELGEDVLLF"
     gene            904..2694
                     /locus_tag="VFH60_12010"
     CDS             904..2694
                     /locus_tag="VFH60_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314550.1"
                     /translation="MSTQPAELTPTNKAWILRQLTISGVLLLVGYMGFFVLMQSSAFY
                     YANLPDPIQIRLHAIFDPINRLFPSTWLNTDRADKSSLVLVLPYLAVMAVIIGTLVYI
                     LRRAGRPDAWRPEYVKPALRRIFGFTAVILVLLLFVRGLLTTDIYSYVWYSRVWVEHG
                     VSPYTHVPAEFAAQDSARWLDWVFWKNEPAVYGPVWVYISGLFYKIGQLANGQFATQL
                     LSLRILADVAHLLNAWLVWKIGGLIVRRRRASPDGTTGTAPDDRQSEGAAPELGRDAG
                     LGFRFGALLFYIWNPLLLVEFGANGHNDVVMLTFVLLAFWLHLSGRWRLAALALGLAT
                     LVKLLALLFVPGYLWLLLWEGYRAAGTRDVLGRFARGALRGAQALAIMAAAWVVLYIP
                     FWEGVQTLRVLVSGPANRWYLHTLSENVVQHGPSFVANIGPWFGVRDTGPEFQGQVRA
                     YIEANLKLWLIPLVGLIAIVVTWKARTFERAMRAWGWVIFAAIIAQAWFWPWYVSWLI
                     APAALASSARLRTATLIFCASSLLNYIEEQVLLWHYSIFRDLSGALIMAPPLIYLLCS
                     WLFEVARNRRARRQAQREVVREQPAVAYGG"
BASE COUNT          485 a          814 c          916 g          529 t
ORIGIN      
        1 gaagagcagc acgtcctctc ctaactcgcc ggcctgggcg cgcgcgtcct gggggaggag
       61 gcgggcgttg tcgagggcgc ggccttcgct ggtgtcctcg aagcgtatga cggcatcggc
      121 tccggggggc acgggcgcgc cggtcatgat gcggaaggcg gtgcccggtt cgacgctatc
      181 acccggctgg gggaaggccc cggcgggcat gtggccggtg acgcgcaggc tgacggggct
      241 atcgtcgccc gcccccacgg tgtcctcggc gcggacggcg taaccgtcca tcgaggagtt
      301 gtcgaagggc gggttgtcca tgtcgctgcg cacctcttcg gccagtacgg tgtccagcgc
      361 ctccatgagc ggcaccccta cggcaggcag cggcgagaag taggagagga tgttctcaag
      421 cgcctcgctg acgctcaagc tggtgagagt aacctggcgc gcttcttctt tcataggacg
      481 cctcgtgtgg gagtagatag cacagcagat tatagcatgt ggtgatgtcg gctcggcaac
      541 aggaccacga ccggagggca aacgataggg acccaggtat tttcgccaga gaggctatag
      601 gcggccgctt agtagggacc aacctcaaaa cgcatgggtt gtgtgttaag tgcgtcttga
      661 ggttccgctc gcaggccggg gacgccctgc cactgaagtg gcgggctggc aagaggcaaa
      721 gtccccttcg gggactaagc aacatgtagt agtctccgaa ggagactttg tctctggtca
      781 gcccgtggct ttagccacag ggcacccccc acagggccaa cctggggcaa gcaatggccc
      841 agaatgtgca aatctgcctt gtataacgag caagcgtagc gtgcgtgagg atcggccctg
      901 ctaatgagca ctcaacccgc agaactcacc ccgacaaaca aagcctggat tttgcggcaa
      961 cttaccatct ccggtgtcct cctcctggtg ggctatatgg ggttcttcgt cttgatgcag
     1021 tcgtcggcct tctactacgc caacctgccc gaccctatac agatccgcct ccacgccatc
     1081 ttcgacccga tcaacaggct tttcccatcc acttggctga acacggaccg cgcggacaag
     1141 tcctcgctgg tgctggtact gccgtacctg gcggttatgg ccgtcataat aggcacgctg
     1201 gtctacatcc tgcggcgagc ggggcggccc gacgcctgga ggccggagta cgtcaagccg
     1261 gccctcagga ggatattcgg ctttaccgcc gtcatactcg tgctgttgct cttcgtgcgc
     1321 ggactcctca ccaccgacat ctattcgtac gtgtggtatt cgcgggtatg ggtggagcac
     1381 ggagtgtcac cttacacgca cgtcccggct gagttcgcgg cgcaggacag cgcgcgctgg
     1441 ctcgactggg tcttctggaa gaacgagcct gcggtgtacg gacccgtttg ggtctacata
     1501 tcggggctgt tctacaagat cggacagcta gcgaacgggc aattcgccac acaattgctg
     1561 tcgctgagga tactggcaga cgtcgctcac ctgctgaacg cctggctggt gtggaagata
     1621 ggagggctaa tagtgcggag gcggcgggcc tcgccagatg gcaccaccgg gaccgccccg
     1681 gacgaccggc agagcgaagg agcggccccc gaactaggcc gtgatgcagg gctgggcttc
     1741 aggttcgggg ccttgctgtt ctacatctgg aacccgctgc tcctggtgga gttcggggcg
     1801 aacgggcata acgacgtggt catgctcacg ttcgtgctgc tggccttctg gctgcacctg
     1861 tcgggtaggt ggaggctggc cgcgctggcg ctcgggctgg caacgctggt gaagttgctg
     1921 gcgctcctgt tcgtgcccgg ctacctgtgg ctgctgctgt gggagggcta ccgggcggca
     1981 ggtacgcggg acgtgttggg ccgtttcgca aggggggcgc tgaggggagc gcaagccctc
     2041 gctatcatgg cggctgcctg ggtggtgctt tacataccct tctgggaagg cgtgcagacg
     2101 ttgcgggtgc tggtatccgg ccccgccaac aggtggtact tgcacacgct gagcgagaac
     2161 gtagtgcagc acggccccag cttcgtcgcc aacatcgggc cgtggttcgg ggtgcgtgac
     2221 accggtcccg agttccaggg gcaggtacgg gcctacatcg aggcgaacct caagctatgg
     2281 ctcataccgc tcgtgggact catagcgata gtcgttacgt ggaaggcacg caccttcgag
     2341 cgggcgatgc gggcctgggg ctgggtgatt ttcgcggcca tcatcgcaca ggcgtggttc
     2401 tggccctggt acgtgtcgtg gctgatagcg cccgccgcgc tggcctcgtc ggcgcggctg
     2461 cgcaccgcca cgctcatatt ctgcgcgtcg tcgctgctga actacattga agagcaggtg
     2521 ctcctgtggc actacagcat attccgcgac ctgagcggcg cactcatcat ggccccgccc
     2581 ctgatctatc tgctctgctc gtggctgttt gaagtagcac gcaacaggag agcacgcagg
     2641 caagcccagc gcgaggtagt tcgagaacag cctgccgtag cctacggtgg atgagcattg
     2701 cgacaatagc aacatctaaa aagacggaac cgccaagacg ccaa
//
LOCUS       DASRWZ010000757         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_404613, whole genome shotgun sequence.
ACCESSION   DASRWZ010000757 DASRWZ010000000
VERSION     DASRWZ010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404613"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..717)
                     /locus_tag="VFH60_12015"
     CDS             complement(208..717)
                     /locus_tag="VFH60_12015"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6314551.1"
                     /translation="MFKHEGRGREGTVERYERETPIAPLGNQINYSEMVLTQVRSAPT
                     TLGWKSVFVTVVLALACAGVTSVFYMLRVPSSDKFAAALVQSHIQEGDRLYGVGLYDM
                     AIVEYNRAIVGRSDYDLAYYRRAEAYEAKGDRARAAADYRKVLEISPDEAMKQEATSR
                     LQRLEAPSP"
     gene            860..1948
                     /locus_tag="VFH60_12020"
     CDS             860..1948
                     /locus_tag="VFH60_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HET6314552.1"
                     /translation="MTSINELDTPALLVDLQRLQRNIASMQAMCDANGVSLRPHTKTH
                     KSPAVARLQVAAGAKGITVAKVGEAEVMVEAGFDDLFIAYPLIGESKYRRLMPLLERA
                     RIAVAADSQEGVAQMSAYFGPLGASLPVLVEIDTGFRRTGVSGAEAAVALAQVIDRAP
                     GLTFAGLMEFSGHSYAACSEEERRAVGHSEAAPLLEAAERLAKMGLPAGVISTGSTPS
                     MPFVAEIAGVTEVRPGVYVFGDLKQAELGTLRRDECALTVLATVVSTPAPGRYILDSG
                     TKALSSDHYATWTYGELKEYPGAVVTRASEEHGIIEAVSLPLRVGDRVEVIPNHACAT
                     CNMHDEFYLTDGDVVLDVWPVLGRGKFR"
BASE COUNT          383 a          656 c          666 g          440 t
ORIGIN      
        1 gatccttcgc tacgctcagg atgacagtgc ctcccctggt cctttactgg taacttactt
       61 gcaagacacc ccaccgagcg tggcgaagca tctagaatgc ctaatgtgca gcttcattga
      121 gcgtcagcac tgttatctct acgttcaacg ctatcctatt accaacttct tgagattctt
      181 cacttcgttc agaatgacag gtcgccgcta cgggctgggc gcttccagtc gctgcaacct
      241 tgatgtcgct tcctgcttca tcgcctcgtc ggggctaatc tctagcacct tgcgataatc
      301 ggcggcggcg cgggccctgt cgcccttggc ttcgtatgct tcggcccgcc tgtaatacgc
      361 caggtcgtag tctgagcgac cgactatggc cctgttgtac tccactatgg ccatgtcgta
      421 cagccccacg ccgtacagcc tgtcgccttc ttgtatgtga ctttgcacga gcgcggcggc
      481 gaacttgtcg gacgagggca ccctgagcat gtaaaacacc gaagtgaccc cggcgcaggc
      541 gagcgcgagc accaccgtta cgaacaccga cttccagccc agggtcgtag gggccgagcg
      601 cacctgcgtc agcaccatct cggagtagtt gatctggttg ccgagagggg ctatgggggt
      661 ttccctctcg tagcgctcta ctgtgccttc tctgcctcgc ccttcatgtt tgaacatatt
      721 tgcgctttcc cacccggccg tatgaggatg ccgggaggct aaggtgtgtc tactcttaac
      781 acaacaaatc gaggggaaag ggtcgttttc gcggtatact ggtaccggcc cgtgccaaaa
      841 ttcacgtcgg gagtagcaga tgaccagtat caacgagctt gacacgcccg ccttgctagt
      901 ggacctccag cgcctgcagc gtaatatcgc ctccatgcag gccatgtgcg acgcgaatgg
      961 cgtatcgctc cgcccgcata ccaagaccca caagtccccc gcggtggcgc gtttgcaggt
     1021 ggcggcgggg gcgaagggca tcaccgtcgc caaggtgggt gaggcggagg tcatggtcga
     1081 agcgggattc gacgacctgt tcattgccta cccgctcata ggcgagagca agtaccgtcg
     1141 gctcatgcct ctgctggaac gtgcgcgtat cgcggtggcc gccgactcgc aggaaggtgt
     1201 agcgcaaatg tccgcctatt tcgggcctct gggtgcgagc ttgccggtgc tggtggagat
     1261 agatacgggc ttcaggcgca cgggtgtgtc cggtgccgag gctgcggttg ccctggccca
     1321 ggtgatagac cgcgcgccgg gtctcacgtt cgcggggctg atggagtttt cgggccactc
     1381 ctacgcggcg tgctcggagg aggagcggcg ggcggtgggt cactccgagg cggccccgtt
     1441 gctggaggcg gcggagcggc tggcgaaaat gggcctgccc gcaggcgtga tctccaccgg
     1501 ctccacccct tccatgccgt tcgttgccga gattgcgggc gtgaccgagg tgcggcctgg
     1561 cgtgtacgtg tttggcgatc tcaagcaggc cgagcttggc accctccggc gcgacgagtg
     1621 cgcgctgacc gtgctggcta cggtggtgag cacccccgcc ccaggccgct acatcctcga
     1681 tagcggcacc aaagccctgt ccagcgacca ctatgcgacc tggacctacg gcgagctaaa
     1741 ggagtaccca ggtgccgtcg tcacacgcgc cagcgaagag cacggcatta tcgaagccgt
     1801 gagcctgccg ctacgagtgg gcgacagggt ggaggtgata cccaaccacg cctgcgctac
     1861 ctgcaacatg cacgacgagt tttacctcac ggacggagat gtggtcctag acgtgtggcc
     1921 ggtgctaggc cggggcaagt ttcgctagac gttcgggagg cagagggggt gccctgccaa
     1981 tgaattggcg ggctgaccag agacgaagtc tccgtaggag actaggcttg tctgcaaagt
     2041 cccgcctctg cgggaccaac ttcccctctg tcattctgag cgtagcgaag aatctcgtcc
     2101 tccagcaccc aaactgccac ttttctttac gtaggtaagg tgcca
//
LOCUS       DASRWZ010000758         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_951973, whole genome shotgun sequence.
ACCESSION   DASRWZ010000758 DASRWZ010000000
VERSION     DASRWZ010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951973"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..1227
                     /locus_tag="VFH60_12025"
     CDS             388..1227
                     /locus_tag="VFH60_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314553.1"
                     /translation="MEEHDEPFGWWGWHWQPPVPLSVMQIIDAGSADTGLMALVWAML
                     ARRASIIVAAEPPMAGKTTTLTALLDLMPPDTRRVYLRGHYETFDFTLEPDAEPHKTY
                     VLANEMSDHLAVYLWGSRVYKMFELITKGYAIGSTMHAETVNDVIAILDSEPLSVPAD
                     WIARLTLVVNLFVSSTYGSAVRRFNTVHMLGPGNHDGESGLLPGVRPLLLSSWDRATD
                     TFDHRFGRPEIEAHLAAWACCSVEEWRADVGCRKAHLDNMLEQGVRGISATHAALRKL
                     KTD"
     gene            complement(1335..2012)
                     /locus_tag="VFH60_12030"
     CDS             complement(1335..2012)
                     /locus_tag="VFH60_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314554.1"
                     /translation="MPGTGLGVAGGGGGAVVGSVGGMVGGGCVGAMVGGMAGRGVAVL
                     AGAGVDEGAGFTVSAALGVGVAGIAVEVAVATGHEATVGGTASGDFTFGTGVGDETEA
                     VTVPPVAVEMVREAVSLPGIAVGEPSAELFICVTPAATTSARTRARLSTPPIALLGAA
                     CLGSSLAARGVVIGAVLAGTRVVGSRARPHRAHASLPAFIGEWHRGHTLGESMKLQQP
                     LSFGLRR"
     gene            2002..>2393
                     /locus_tag="VFH60_12035"
     CDS             2002..>2393
                     /locus_tag="VFH60_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314555.1"
                     /translation="MPGIGGTANDWNGWAIGLATMAAHQQINSQWGVIYAPSGTFWLI
                     WIDARNDYTATRSLGATVDFLLQDGNGNLYTELSDHGKGQDTRYIAPIFGRDYLNARV
                     APGGVTRTLLVFDLPPNATPAELIGRRI"
BASE COUNT          485 a          850 c          630 g          428 t
ORIGIN      
        1 tgatccctga tccctatttc aagcaaagga cgcaatatgt tgcatatcac taacggcgag
       61 tcggcggggg aaaggatcag gcagtcgggg gtggctgggc ggggtgcatc tacagggcaa
      121 ggatgccgcg tggcggtggc atggcaggga gaagaggctt gttcccatat aaacatcata
      181 cgcgatagca gaagccaagc cccggaggct gtatgcaccc gcttctcagt aagaacttgg
      241 tctgagcttt atcgcaccct acacctcaca accgaactta acgtataatt atatagccct
      301 ctatatgagc gtacccattc cccacactcc accaattacc aacccagcac acatcacgtt
      361 tatcgcaaga cgcaatacgc caaccccatg gaagaacacg acgagccgtt tggctggtgg
      421 gggtggcact ggcagccgcc ggtaccactc tcagtaatgc agataattga cgcgggcagc
      481 gccgatacgg gcctgatggc gctcgtgtgg gccatgctcg cacgccgcgc ctccatcatc
      541 gtggcggctg agcctcctat ggcgggcaag accaccaccc tcaccgcgct cctcgacctc
      601 atgccccccg acacgcggcg cgtctacctg cgcgggcatt acgagacgtt cgatttcacc
      661 ctcgaacccg acgccgagcc ccacaagacc tacgtgctcg ccaacgaaat gagcgaccac
      721 ctggcagtct acctctgggg ttcccgcgta tacaagatgt tcgagctaat cacgaagggc
      781 tacgccatag gctccaccat gcacgccgag accgtcaacg acgtaatcgc catcctcgac
      841 tccgagcctc tgagcgtgcc cgccgactgg atagcccgcc tcaccctggt cgtcaacctc
      901 ttcgttagca gcacttacgg ttcagccgtg cgccgcttca acaccgtgca tatgcttggg
      961 cctggcaacc acgacgggga aagcggcctc ctcccaggcg tgcgccctct cctcctgtca
     1021 agttgggacc gcgccacgga caccttcgac caccgctttg gccgccccga aatagaggcc
     1081 cacctggcgg catgggcttg ctgctccgta gaggagtggc gcgcggacgt aggctgccgc
     1141 aaagcccacc tggacaatat gttggagcaa ggcgtccggg gcataagcgc cacccacgcc
     1201 gccctgcgca agctgaaaac ggattagcag catggccact aatacgattg tcactctaca
     1261 aatacctcct tcgagcatag cgtgggatat gggctttgtg gtataaataa cttgccctac
     1321 tcggtgccgg cgtgttacct tcttaacccg aacgatagag gctgttgtaa tttcatgctt
     1381 tcacccaacg tatgcccgcg atgccattcc cctataaagg ccggaaggct tgcgtgcgcc
     1441 cggtgcggcc tggccctcga cccgaccacc cgcgtcccgg ccagaacagc gcctatcacc
     1501 acgcctctgg ccgccagtga gcttcccagg caggccgcac ccaacagggc aatcggcggc
     1561 gtgctcagcc tcgccctggt cctggcgctg gtcgtcgcgg cgggcgtgac gcagatgaat
     1621 agctctgccg atggctcccc cactgcgatc ccaggcaggc ttaccgcctc ccgcaccatc
     1681 tcaaccgcca ctggggggac tgttactgct tcagtttcat cgcctacacc ggtgccgaac
     1741 gtgaagtcac ctgaagccgt gccgcccaca gtggcttcgt gacctgtcgc tactgctacc
     1801 tctaccgcta tccctgccac tcctaccccc agggctgcgg acacggtgaa gcctgcgcct
     1861 tcatcaacac ccgctcccgc gagcacagcc acgccccgcc ccgccatccc acccaccata
     1921 gcgccgacgc agcccccacc taccattcca cccacgctgc cgaccacagc gccgcctccg
     1981 ccgcctgcca ctcccaggcc tgtgcccggc atcggcggga cggccaacga ctggaacggc
     2041 tgggcaattg gcctggctac tatggccgca caccagcaga taaactcgca atggggcgtt
     2101 atttatgccc catccggcac tttctggctc atctggattg atgcccgcaa cgactacacg
     2161 gccacccgct ctctgggcgc aacggtagac ttcctcctcc aagacggcaa cggcaatctg
     2221 tacaccgaat tgtcggatca tggtaagggg caggacacac gatacatagc gccgatcttc
     2281 gggcgcgact acctcaacgc tcgcgtggcc ccaggaggcg tcacacgcac cctgctcgtc
     2341 ttcgacctcc cccccaacgc aacccccgcc gagcttatcg ggaggcgcat cat
//
LOCUS       DASRWZ010000759         7279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1157641, whole genome shotgun sequence.
ACCESSION   DASRWZ010000759 DASRWZ010000000
VERSION     DASRWZ010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157641"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..2799)
                     /locus_tag="VFH60_12040"
     CDS             complement(373..2799)
                     /locus_tag="VFH60_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6314556.1"
                     /translation="MPKANSIAHIPQANGHAPGKDAPRSLPAQVTPFIGRQREVAGVR
                     ELLHDAGVRLLNLTGPPGIGKTRLAVQVAAGVAGRFPGGVYFVSLAAVTRPEGIVAHL
                     AQALGLREMSNQPLLQRLQEHLADKQTLLVLDNLEQIEGPAATIGSLLAACPRLKMLA
                     TSRTRLQVYGEHEYRVPALGLPDAERLPNLQSLSKVEAVALFVQRAQAARADFRLTEA
                     NAEAVARLCVGLDGLPLAIELAAARTRTLSPQAMLSRLHHRLDLLGGGPRDLPARHQT
                     LREAIAWSYDLLTPEEQQLFRRLAVFSGGATIEATRHVAGERDGGHTASACGEALVES
                     LHDKSLLAQPAQGEQAEAEARFSMLETVREYAWERLAESGELEAARKAHALYYEALAD
                     MRTEVVVSPAGKALLERLEREYDNVRAALAWCANTQDPEGTDIGMRLAGGMSLFWDIR
                     GYLKEQYEQVMAMLSKAEQATDRHALARLLIAAARSVTLLGIEVVGKSYLEESLALSR
                     EAGEKHAIVVALSALGHAAVHLDDLALAESCFEECLALHAESGDTCGLATILSKQADL
                     ALHGGDIAGARVKFEEALAVARDVYAPMLLADLLIYTGYVSAEEGEYEQAIENVSQAI
                     EMVREMEAVQTIAYAVGVLGKVAYVQGQHEQAVRLYAAASTLFDRCGLPLEPPLHTDP
                     AEYRRYLEELSGTLGDKTFAAAWERGRKLGVPEVLAELRNAPAQSAPETAQPQPGVPN
                     ASALPAGLTPREVEVLRLVASGMTNSEAASALTVSPHTINMHLRSIFSKLGVTSRSAA
                     TRFALINGLV"
     gene            3086..4018
                     /locus_tag="VFH60_12045"
     CDS             3086..4018
                     /locus_tag="VFH60_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314557.1"
                     /translation="MDDDARFAPLQEAQERLNRLLAGGHTPVEGGQQAEGEGQEGRAD
                     VPVELPIAAVYIDRDNNQLVIGLSAGEEANRSRHEEQIERLLGDVPRRLTYVTVVRDD
                     APNKKQACRPLWGGVRVQSGATLSVVYDLRNGTMGTILSGHGTGGVGTSVGQPGAPDT
                     YGKVTINPPLTYRASDSALAVITNQRIQGQPNAIWIAPNQSITVSDYAISANTPLRLV
                     IYMQGAQTDEIQRGVIYAKGVTVRDARGTLVNQVLGTYSAQGGDSGAPVFYVVNSYAV
                     YVGIHAGRMQMDQGMMPFYSPWEAIRSELKLPQY"
     gene            complement(4085..5257)
                     /locus_tag="VFH60_12050"
     CDS             complement(4085..5257)
                     /locus_tag="VFH60_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6314558.1"
                     /translation="MTTYIVRRFAQSLAFGALASLLIFTVLVYVMPNGPYQTYKSVLP
                     FQHNPDSRRFLKQITVAYPVDKPWPLSFLAWLYDPNDTTTIDPLTEQEVPKGVDIVIG
                     GLHIRGDGALLGELGNSFYVFRGQPVRELIGSRWGNTVSLLGLAFFLSLVVAIPVGLI
                     GAISHNSKLDHSLTFFSFLGMSVPPFALGLILIIFLAVIPFQLRLSNGWEWLPYFPPG
                     AVASLGQEESLINRLYHLALPAITLALGQIAWMSRHVRFSMLDVLRRDYVRTARAKGV
                     RGWRVISKHAFRNALIPLITIIGLSVPALATGAIVVEKLFGYPGLGYLLFFSLGGCTP
                     TPGSETPCPPNSAFPDTPLALTLTLILIFVVTFANMLADIFYAVADPRINDNTGQG"
     gene            complement(5566..6591)
                     /locus_tag="VFH60_12055"
     CDS             complement(5566..6591)
                     /locus_tag="VFH60_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6314559.1"
                     /translation="MATAISTEVQAEIPGRRREDAVGQSQVAIVWRNFRRRKVGIVGL
                     VMLGLLIVLSVAVPIFSPFGYSATSGPQLWYAPFGAPDPNGHSHLLGTDSLGRDLMTR
                     LFFAGRISLSLAVPSALGVMLLGCIIGAIAGFYGGLIDAVLMRATDFLLSLPLIPMYL
                     YTSQFLKATFIPQTGEPLNTERLAENTLVILGLTVLVFTMYGWMGVSRLVRGSILSLR
                     SQPFVEAARALGASDRRIIFRHLLPNAMAPILVAATFAVGDFVIIESLLSYMGQGATD
                     TFAPSWGNLLAVSEENVWYLTSLNPFENVKAFTILMPSFMILFTVLSFNYIGDALRDA
                     LDPHERA"
     gene            complement(6859..>7279)
                     /locus_tag="VFH60_12060"
     CDS             complement(6859..>7279)
                     /locus_tag="VFH60_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314560.1"
                     /translation="RLSRRHHLGGYPCGGPGEPCDNQSRPYFRPAANATRGQLSKIVS
                     NAASFNDTPQGQTYTDVPPSTSESSFYPFIERLSSRGVMGGYPCGSPGETCDDQNRPY
                     FRPHTLVTRAQSAKIVSNTFFPGCTIGTEAQQAAVKK"
BASE COUNT         1350 a         2185 c         2434 g         1310 t
ORIGIN      
        1 cttactctac cggcaaacgg ccgcgtggca cattacttat tcgagtagtt ttggtaggac
       61 cgtgaggggc tatgcgttcg agcaggtcgg ggcatgagga aaggtggtac ttgggtgctg
      121 gaggacgaga tttctcacgg agtttaccct gagcgtagcc gaagggttcg aaatgacaag
      181 ggtggggctt tggttctccg gaagtagcta tcaagagcgg gtggctgatc cttcgctatg
      241 ctcaggatga caaaggggag tatggtcttc ggtcacgaag caggaaatca ggccggccca
      301 tgaccgcaga accttcgctt tctcagcgta acctctgcgt cctctgagtc tctgcggtga
      361 aaaaatgtcg ccctacacca ggccgttgat gagggcgaag cgggtggcgg cgctgcggga
      421 ggtgacgccc agcttgctga agatggagcg gaggtgcatg ttgatggtgt gggggctgac
      481 ggtgagggcg gaggccgctt cggagttggt catgccggag gccaccaggc gcagcacttc
      541 tacctctctg ggggttagcc cggcgggtag cgcggaagcg ttggggacgc cgggttgggg
      601 ttgtgccgtc tccggggcac tctgggcggg cgcgttgcga agctcggcca gcacctccgg
      661 cacgccgagc tttcgccccc tctcccaggc ggcagcgaac gtcttgtctc ccagggtgcc
      721 gcttaattct tccaggtagc ggcggtactc ggcggggtcg gtgtgcaggg gcggctccag
      781 gggcaggccg cacctgtcga acagggtcga agccgcggca tagaggcgca ccgcctgctc
      841 gtgttgcccc tgcacgtacg ccaccttgcc gagcactccc acggcatagg cgatggtctg
      901 gacggcctcc atctcccgca ccatctcaat ggcctgggat acgttctcaa tcgcctgctc
      961 gtattctcct tcctcggcgc tcacatagcc cgtatagatg agcaggtcgg ccaggagcat
     1021 cggggcgtag acgtcgcggg ccacggccag ggcttcctcg aactttaccc tggccccggc
     1081 aatatcacca ccgtgcaagg ccaggtctgc ctgcttgctc aggatggtag cgaggccgca
     1141 cgtgtcgccc gactcggcgt gcaacgccag gcactcttca aagcaggact ccgccagggc
     1201 aaggtcgtcc aggtgcacgg ctgcatgccc cagcgcgctc aaggccacca cgatggcatg
     1261 cttctcaccg gcctccctag acagcgccag gctctcctct aggtagctct tgcctaccac
     1321 ctcgatgcct agcagggtta cggacctagc ggcggcaatc agcagccggg ccagggcgtg
     1381 cctgtcggtg gcctgctctg ccttgctcag catggccatg acctgctcgt actgctcctt
     1441 caaatagcct cgtatgtccc agaaaagcga cataccgcct gcgaggcgca tacctatatc
     1501 ggtaccttcc gggtcctggg tattcgcgca ccaggcgagt gcagccctga cgttgtcgta
     1561 ctccctttcc agccgctcca acagcgcctt gcccgcgggc gagacgacta cttcggtacg
     1621 catgtcggca agggcctcgt agtagagcgc gtgcgccttg cgggccgcct caagctcgcc
     1681 actctcggcc agccgctccc aggcgtactc ccggaccgtc tcaagcatcg agaagcgcgc
     1741 ctcggcctca gcttgctccc cttgcgcggg ctgggcgagc aggctcttgt cgtgcaggga
     1801 ctccaccagg gcctccccgc aggcgctcgc tgtatggccg ccgtcacgtt cgcccgcgac
     1861 gtgccgggta gcctcgattg tcgcgccgcc ggagaagacg gccagccttc ggaaaagctg
     1921 ttgctcctcg ggggtgagga ggtcgtagct ccaggcgata gcttccctca gcgtctggtg
     1981 ccgggcgggg aggtcacggg ggccgccgcc aagcaggtct agcctgtggt gcagccggga
     2041 gagcatcgcc tggggagaga gggtgcgggt gcgggcggcg gcaagctcaa tggcgagggg
     2101 cagtccgtct agacccacgc acaggcgggc gactgcctcc gcattggctt cggtgagccg
     2161 gaaatcggcc ctggccgcct gcgcccgctg cacgaacaag gcaaccgcct cgaccttcga
     2221 gagggattgc aagttgggca gtcgctcggc gtcgggcagg cccagagcgg gcacgcggta
     2281 ctcgtgctcg ccgtagactt gcaggcgggt ccggctggtt gccagcattt tgagccgggg
     2341 gcacgcggcg aggagcgacc ctatagtggc ggcgggcccc tctatctgct ctaggttgtc
     2401 gagtaccagg agggtctgct tgtctgccag gtgctcctgc aatcgctgga ggaggggctg
     2461 attgctcatc tcccgcagcc ccagcgcctg ggcgaggtgc gcgacgatgc cctccggccg
     2521 ggtgacggca gccagcgaca cgaagtagac tccaccgggg aagcgcccgg ccacaccggc
     2581 ggccacctgt accgcgaggc gggtcttacc tatccctggc ggccccgtga ggttcagcag
     2641 gcgcaccccg gcatcgtgga gcaactcgcg cacgcccgcc acttcgcgct ggcggcctat
     2701 gaaaggtgtg acctgcgcgg gcaagctcct cggcgcgtcc ttgccgggtg cgtggccgtt
     2761 ggcctgggga atatgtgcta tggagttggc ctttggcatc tcgcccggtc ctcgtctgca
     2821 taaagctgag tcgggagccg atatagtccc ttctctgctc tatatcacag ccaatacccg
     2881 aaaacggttg actggaccct accggtgcta ctctgctgga aggtatcgga cggcacgtgc
     2941 cgaacattta ggtcgtaagt ttatggaagt aattaatcat tgtattatag ctatatgtgc
     3001 tctatactca agatgctaaa ctgcaatact aatcaccctg gcgcggccca cgtacttcag
     3061 gctctacgag caaggagcga cagacatgga cgatgatgcg agatttgccc cactacagga
     3121 ggcccaggaa cggttgaacc ggttgctggc gggcggacac accccggtgg agggcggcca
     3181 acaagccgag ggcgagggcc aggaaggtag agccgacgtg ccggtggaat tgcctatcgc
     3241 ggcagtctac atcgatagag acaacaacca gctcgtgatc ggcctgtccg ccggagaaga
     3301 agctaaccgc tcgcggcatg aggagcaaat tgagcggttg ctcggggacg ttccgaggcg
     3361 acttacctat gtgaccgtcg tgcgcgacga cgcgcccaac aaaaaacaag cgtgccgccc
     3421 actgtgggga ggcgtgcggg tgcaaagcgg ggccactttg agcgtagtct atgacctgcg
     3481 gaacggcact atgggcacga tcctatcggg ccacgggacc ggcggtgtgg gtacgtcggt
     3541 tgggcagcct ggggcgccgg atacgtatgg aaaggtgact atcaacccgc cattgaccta
     3601 ccgcgccagc gactccgcgc ttgccgtgat caccaaccag cgtatccagg ggcagcccaa
     3661 cgcgatatgg atcgccccca accagtctat tactgtgtcg gactacgcca tctcggcgaa
     3721 tacacctctc cggctggtta tctacatgca aggcgcgcag accgacgaaa ttcaacgagg
     3781 tgttatctac gccaaggggg tgaccgtcag ggacgcgcgg ggtacgctgg tcaaccaggt
     3841 cctggggact tactcagctc aaggcggcga cagtggagca cctgtattct acgtggtcaa
     3901 cagctacgcc gtgtacgtcg gcatccatgc agggcggatg cagatggacc agggcatgat
     3961 gcccttttac agcccctggg aagcgatcag aagcgaacta aagctgcccc agtactagag
     4021 accaggtaga gagtttaccc tgtgcagggc ctcactcgcc acgcattacc cacccatcgt
     4081 cttcttatcc ctggcccgtg ttgtcgttga tcctggggtc ggccacggcg tagaagatgt
     4141 cggccagcat gttggcgaac gtgaccacaa aaatgaggat gagggtaagg gtgagggcca
     4201 ggggtgtgtc ggggaaggcg ctgttggggg ggcagggggt ctccgagccg ggggtggggg
     4261 tgcagccgcc cagcgagaag aagagcaggt agcccaggcc ggggtagccg aacagcttct
     4321 ccaccactat agccccggtc gccagggccg gtacggacag gccgattatg gtgatgaggg
     4381 ggatgagggc gtttcggaag gcgtgcttag agatcacccg ccagccgcgc acgcccttgg
     4441 ccctcgccgt gcgcacgtag tcgcgccgca gcacgtcgag catcgagaag cgcacgtgcc
     4501 gcgacatcca ggctatctgc cccagcgcca gcgtgatagc gggcaaggcc aggtggtaca
     4561 gccggttgat gaggctctcc tcctgcccca ggctggccac cgcgccgggc gggaagtagg
     4621 gcagccactc ccagccgttg ctcaagcgca actggaacgg tatcaccgcc aggaagataa
     4681 tcaggatgag gccgagcgcg aagggcggca ccgacatgcc caggaacgag aagaaggtca
     4741 gcgagtggtc cagtttcgag ttgtggctga tcgcgcctat gaggcccacc ggtatggcga
     4801 cgaccagcga caagaagaag gccagcccca gcagcgacac ggtgttaccc cagcggctgc
     4861 ctatcaactc gcgcaccggc tggcccctga acacgtagaa cgagttacca agctccccca
     4921 gcaaggcccc atcgcccctg atgtggaggc ctcctatcac gatgtcaacg cccttgggga
     4981 cctcctgctc ggtgagcggg tcgatggtcg tggtgtcgtt ggggtcgtac agccaggcga
     5041 ggaagctgag gggccagggc ttatcgacgg ggtaggccac cgtgatttgc ttgaggaagc
     5101 gccggctgtc ggggttgtgt tggaagggca ggacgctctt gtaggtctgg tacggcccat
     5161 tcggcatgac gtacaccagc acggtaaaga tgagcagcga ggcgagcgcc ccgaaggcca
     5221 ggctctgcgc gaaccgccgg acgatgtaag tggtcatacg tgtttacctc tgcgaagtaa
     5281 aagcatagat ctatgtcaag ccgtattaaa gtacccggcg caccaaccgg cgtaccaacc
     5341 gatgcgctat tgccgtccta agcgcggcga ccatactccc ctctgtcatc ctgagcgccg
     5401 cgaaggatca gccgcccgtt cttgactctc gctacttgag caccaatgct cctaatgccg
     5461 ccagcatccc cagcgttgta gactctggcc tacccacctg atccttcgct gcgctcagga
     5521 tgacaagggg cggggcctgg tacagcacaa gggacagcaa tcgcgttacg cgcgctcgtg
     5581 ggggtccagg gcgtccctga gggcgtcgcc tatgtagttg aaggagagca ccgtgaacag
     5641 gatcatgaag ctcggcatga ggatggtgaa cgccttgacg ttctcgaacg ggttgaggct
     5701 ggtgaggtac cagacgttct cctccgacac ggccagcagg ttgccccagg aaggcgcgaa
     5761 cgtatcggtc gccccctgcc ccatgtagga gagtagcgac tcgataatca cgaaatcgcc
     5821 caccgcgaag gtcgccgcca cgaggatggg ggccattgcg ttaggcagca aatgccggaa
     5881 gatgatgcgg cggtcgctgg caccaagcgc cctggcggcc tccacgaagg gctggcttcg
     5941 cagcgacagg atggagccac gcaccagcct gctcacgccc atccagccgt acatggtgaa
     6001 cacgagcacc gtcaggccca gtataaccag cgtgttctcg gccaggcgct cggtgttgag
     6061 cggctcgccc gtctgcggga tgaaggttgc cttgaggaac tggctggtgt aaaggtacat
     6121 cggaatgagg ggcagggaga gcaggaagtc ggtagcgcgc atgagcaccg cgtcgatgag
     6181 gccgccgtag aagcccgcta tcgccccgat gatgcagccg agcagcatga cgccgagcgc
     6241 cgagggcacc gcgagggaga gggagatgcg cccggcgaag aagagccggg tcatcaggtc
     6301 gcgccccagg gagtcggtgc cgagcaggtg ggagtggccg ttggggtcgg gcgcgccgaa
     6361 gggagcgtac cagagctggg ggccgctggt ggcgctgtag ccgaacggcg agaagatggg
     6421 caccgcgacg gacagcacta tcagcagccc cagcatcacc agcccaacta tgcccacttt
     6481 gcgcctgcgg aagttgcgcc agactatcgc cacctggctc tgccctaccg cgtcctcgcg
     6541 ccgcctgccc ggtatttccg cctgcacctc cgtggagata gcagtagcca tcctcacctc
     6601 ctgcgctgtg atgctgctgt gaatggccgc gttgctatgt cgttggtgac atgggattgg
     6661 cggctcacct gtatatatgc aggagtgttt tagaaagttc gagatactag gtagaaatta
     6721 gaacgcgagt tgaacggggc ttgctcttga ttcctggcat tggcactggc ccgctatacg
     6781 agtaaggcca gggccgggtt tggcaaacga aaagggaggc cctgcggggc ctcccttttc
     6841 gtttgcgcct ctagaccgtt acttcttcac cgctgcctgt tgggcttcgg tgccgatggt
     6901 gcagcctggg aagaaggtgt tggacacgat cttcgcggac tgggccctgg tcacgagggt
     6961 gtggggccgg aagtacggcc tgttttgatc gtcgcatgtc tcgccggggc tgccgcacgg
     7021 gtagccgccc atcacgccgc gagaggatag tcgttcgatg aacgggtaga acgacgactc
     7081 gctggtgctc ggcggtacgt ccgtgtaggt ctggccttgc ggcgtgtcgt tgaagctcgc
     7141 agcgttggaa actatcttcg atagctgccc gcgagtggcg ttggcggcag gccggaagta
     7201 cggcctgctc tggttgtcgc acggctcgcc ggggccgccg cacgggtacc cacccaggtg
     7261 gtgcctgcgg gagaggcgg
//
LOCUS       DASRWZ010000760         5964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_472383, whole genome shotgun sequence.
ACCESSION   DASRWZ010000760 DASRWZ010000000
VERSION     DASRWZ010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472383"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFH60_12065"
     CDS             complement(<1..591)
                     /locus_tag="VFH60_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314561.1"
                     /translation="MVGKKVRGLAALTLVAVSLGGVGLAGIPSASAAATTEVITEADV
                     VRQLHPSTPTNEWVIYTRNVASATFRNGPGTPPLGTGSLELSTGDKVYAFNYEHIGTQ
                     LSDIDAISYSTYRDPASTAIPSQVAALNIEVDVNGAAPGGFTTLVFEPVYNLTQGVVQ
                     PGVWQGWDAYNGGNAIWWSSNNIPGAPNRDTFVTWNT"
     gene            complement(1053..3098)
                     /locus_tag="VFH60_12070"
     CDS             complement(1053..3098)
                     /locus_tag="VFH60_12070"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET6314562.1"
                     /translation="MSTTVTPRAGRSLEPFIGPALSLSTLFLYARTLSPSVGGTVDSP
                     EFQHAAYSLSVAHPTGYPLYLLAGRAWITLFPFGDPAYRMNFLSALFAALAVWVLYAL
                     VRHITGSIAAGVGAAALYAVLPIPWSQASVAEVNSLHTLLVGLSFLCVVLWARGRLPL
                     PAVALVLGCAISHHRTALLYVPVLALYALFALKKPGAARVRLADIPLSVLLLLLPLAT
                     YIYLPLRAFTTSWYTNTWAGFIQHVAGESALSMINVGIGRALLGRVRQLLADQIFSGP
                     QGGLVFAIGLAGLGVLVFESLRRWRSRKSNTGANSPWYSFEEVRLGPAAVAVVAFVLG
                     IGFASIYQIFDIVDYLGVPIFMWGVLTGLAFLAAGKLAAFALSRLSTATRAVTVARLA
                     QGLVIALAAVLAFFAATQSLAKSGVQVDYSKLDRQGYWRTVKQELEAAPPGTILIGDW
                     YELNEALYFQRVEGWRPDFVGAPLDSAYREEGAQVDAWLAEGRQVYLLGHYEPVLSRF
                     EAQVRGPVTFGSEVRGPLWQVLGRRTQVATPEMAREVNRRFGSSIVLVGYTLSPEAEM
                     KPGETLHLTLYWQASERVYERYTAFTHVIDERGDKVGQKDDEPQRGYRPTVLWQPGET
                     IADTLEIPISSTTAPGRYRIVTGMYNSVTQQRIEAFAADGTSLGDYVPLAEIVVPTP"
     regulatory      3685..3825
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4002..5099
                     /locus_tag="VFH60_12075"
     CDS             4002..5099
                     /locus_tag="VFH60_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HET6314563.1"
                     /translation="MSTLATRMKKIGAVSMSVLCVLGTFAGGAAAQGKYQGQATAALN
                     WMKTQQQPDGSFAGFGAGSTLDAVLAIMAAGQSPSSYSIDGNTPITFLESKVADLTRN
                     PGSAGKLLIVTGNLNGNGTFGGVDLVGLINSSYDPATGHYGGDALGHAFAMLGLHAAG
                     QTIPPAAVEYLKGTQTPEGGWAFTGDTAPGAADTNTTAVVIQALQAAGATNTQPDLLK
                     KAVTYIDSQQNPDGGFPYQKVGGPDSESDVNSSSYVAQAMLSLGNTTIADRALAFIAS
                     LQKPDGAFQWKPSEPDDTAGATYQAVSALLQTTLVTPLPGTASQGETSSPVPGRPTTG
                     SPVGDLLGTLAALAALALSAGVLVRRGAQGR"
     gene            5126..>5964
                     /locus_tag="VFH60_12080"
     CDS             5126..>5964
                     /locus_tag="VFH60_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314564.1"
                     /translation="MRRSYTTGYGVTRPYTTSRGMPHRALSLLLVIVCVGLAGGSAAG
                     AGSAQSESHAGLVVQFADGTTNTYCLAFTGESITGLDLLLKTGLDVTAETQGALGGWI
                     CKIGPDGCNFPEQPCACQSYVLGGKYWVYSHLKDGVWKTSQLGASNYKVRDGEVEGWA
                     WSTGKGPSVTPSFAELCAAVLPAPPEPTATNPPPPPPPTNTPLPPLPTDTPVPPTSTP
                     QPPAPTATPRPRLTPTPAPAPPTAEVPQQLAPTPTQEPPTQTPTTLPTDTPVPTDTPT
                     TLPT"
BASE COUNT         1173 a         1975 c         1839 g          977 t
ORIGIN      
        1 ggtgttccac gtgacaaagg tgtcgcggtt cggggcaccg ggtatgttgt tcgaggacca
       61 ccaaattgcg ttgccgccat tgtatgcatc ccagccctgc cagacaccgg gctgaacaac
      121 tccctgggtc aggttgtata caggctcaaa gacgagcgtg gtaaacccgc ccggcgcggc
      181 cccattcaca tcgacctcga tgttcagggc ggctacctgt gacggaatag cggtggatgc
      241 ggggtcccgg tacgtagagt agctgatggc atcgatgtca cttagctgag tgccgatgtg
      301 ttcgtagttg aacgcataca ctttgtcgcc ggtgctcagc tccaggctgc cggttccgag
      361 gggcggcgtg cccggcccgt tcctgaacgt ggccgaggcg acgttcctcg tgtagatgac
      421 ccactcattg gtaggggtac taggatgaag ctgacgcacc acgtccgcct ctgtgatgac
      481 ttccgtcgta gccgccgcgc tggcagaggg tatgcctgcc aggccaaccc caccgaggct
      541 aaccgctacg agtgtcagtg ccgcgagtcc tctaactttc ttgccaacca ttcgtaagtc
      601 ctcctgatgt gattacccgc cagccggggt gcttgaggcc ttctgcaaac ccccggccgc
      661 ggcttacgtt acttgtcgcc gtcccgatac caccctgccg gttgtgccct gtttgccgcg
      721 cgggcgtggt caagacaagg ccggttggcc ggatgccata gctcaggctc cgataggcaa
      781 gatggttttg gacagcaaca gaccagccac tgactaaata ggataacagt cagctggccc
      841 aggtcctttg cttgcgcctg tgcgtagctt cgggtttcgc ggcagctttg taacatttta
      901 ctgctaccac ttcgccaggg aactacttac gaggcgaggc cagtatacgt aatactgccg
      961 cttccgtcaa tgccgcgaag tattatcttc taagtaatac cttgaggatg cgtccagcag
     1021 agggcgtgct cgcggttagt ccacacaacc ggctatggcg tggggactac gatttcagca
     1081 agcggcacgt aatcgcccag ggaggtgccg tccgccgcga aagcctcgat ccgctgctgc
     1141 gtcaccgagt tgtacatgcc ggtgacgatc ctgtaccttc caggggccgt ggtgctgctt
     1201 atgggtattt ccagcgtgtc ggctatcgtt tcgcccggct gccagagcac tgttggcctg
     1261 tagccacgct gcggctcgtc gtccttctgc cccaccttat cgccacgctc gtctatgacg
     1321 tgcgtgaagg cggtatagcg ctcgtacacg cgctccgacg cctgccagta gagcgtcagg
     1381 tgcagcgtct cgcccggctt catctcggcc tccggcgaca gggtgtagcc cacgagcacg
     1441 atactactgc caaagcgcct gttgacctcg cgcgccatct ccggcgtggc gacctgcgtc
     1501 ctgcgcccca acacctgcca taaggggcct cttacctccg agccaaacgt gacggggccg
     1561 cgtacctgcg cctcgaagcg actcagcacc ggctcgtagt gccccaggag gtagacctgc
     1621 cgcccctccg cgagccaagc gtctacctgc gcgccctcct cgcggtacgc gctgtcgagg
     1681 ggcgcgccta cgaagtcggg ccgccagcct tccacccgct ggaagtagag cgcctcgttt
     1741 agctcgtacc agtcgcctat gagaatggtg ccgggaggcg cggcctccag ctcctgcttg
     1801 accgtgcgcc aatagccctg gcggtcaagc ttgctgtagt ccacctgcac gccgctcttc
     1861 gccaggctct gcgtagccgc gaagaaggcc agcactgcgg ccagggcgat aaccagtccc
     1921 tgcgcgagcc ttgccaccgt aacggctcgc gtggcggtgc tcagcctgga gagggcaaag
     1981 gccgccagtt tgcccgccgc caggaacgcc aggcccgtca gcacgcccca catgaagata
     2041 ggcacaccca ggtaatcgac tatgtcgaat atctggtata tagacgcgaa accgataccc
     2101 aggacaaacg ccaccacggc tacggcggca ggtccgagcc tgacttcctc aaatgagtac
     2161 cagggagagt ttgcgccggt gttgctcttg cgggagcgcc accgccggag ggactcgaag
     2221 acgagcaccc caagccccgc caggccgata gcgaacacaa gcccaccctg cggcccgctg
     2281 aatatctggt ccgcaagtag ctgcctcacg cggcccagca atgccctgcc tatccccacg
     2341 ttgatcatcg agagggccga ctcgcctgct acgtgctgta tgaacccggc ccacgtgttg
     2401 gtgtaccagg acgtggtgaa ggcccgtagc gggaggtaga tgtacgtcgc cagggggagg
     2461 agcagaagca acaccgagag ggggatgtcc gcgagccgca cgcgagccgc gccaggcttc
     2521 ttgagggcaa acagggcgta cagcgcgagc acgggcacgt acagcaacgc ggtacggtgg
     2581 tggctgatcg cacagccgag cacgagcgcc acggcgggca gggggagccg ccccctggcc
     2641 cataggacca cgcagaggaa ggaaagtccg acaaggagcg tgtgcaggct gttcacttcg
     2701 gccacgcttg cctgcgacca gggtataggg aggaccgcgt acagggcagc cgcgcccaca
     2761 cccgccgcta tgctgccggt aatgtgcctg acgagcgcgt acagcaccca gacagcgagc
     2821 gccgcgaaca gggcagagag gaagttcatg cggtaggccg ggtcgccaaa ggggaagagc
     2881 gtgatccagg cccggcccgc gagcaggtac agcgggtagc cggtggggtg cgcgaccgac
     2941 agcgaatagg cggcgtgctg gaactcaggg ctgtccaccg tgccaccgac cgagggcgac
     3001 agggtgcggg cgtagaggaa gagcgtgctg aggctcaagg ccgggccgat gaaaggctcc
     3061 aggctgcgcc ccgccctggg tgttacggtg gtgctcatgc tggtggatag ccttccttgc
     3121 gggacataga agcgcaggtc tcgtacggcg gggtgggtgt ggtggccctg ggaagtatag
     3181 caccgggcag ttggtggtgc aaagcgggct gcacaggagg gagaccctag tcgaggtcac
     3241 tcgtggacta gcccgcaatg cggcatactg acaaattatt caacatattg tgccagccgc
     3301 ctgctaggga tgcaaataaa ccccatagtc gccagccgac gcccgtcgat tgaaatcgcg
     3361 ggctacacaa tacgaagtcc cacaagggga ctccttctca gctagatatt cgtgcaggag
     3421 agactcagtc gcacgtagcc agtctgccgc agggcagact ttgtatgtgt agcccgcgat
     3481 ttcaatcgcc gggcgtctcc tggcaccgtg tgcggcttgc atcctcagcc ccctggtcgc
     3541 ctggagattg tgctgctcgt atatgcttga cctgcataat cggcaaggca tcctacgcag
     3601 gacatctgct cccacaattc actaacaact aacaactctt gactccaccc caccccggtg
     3661 ctatactcag cctgttaaca accgaatacg agaaagcgca gaggtcgccc gaaacacgga
     3721 agtaagggcg gcaatggggg aagccggtaa aagtccggcg ctgtcccgca actgtaatct
     3781 ctacaggaga aagccaggat acccgcctct gtgtcactcc agttaccttc gcgagagagg
     3841 gagaggagga gcgatgacgc agccacgcac gcatagccca ccgtaggcac tgtcatcccc
     3901 aactgtagat agaagcatct acacacgcaa acccttgtcg caccggcaag ggcttttttg
     3961 tttgtcacgc gacacttaca tttcgtgtgg aggagaaaca gatgtctaca ctggcgacaa
     4021 gaatgaagaa gataggggcc gtatcgatgt ccgtgctgtg cgtcctgggg acgttcgcag
     4081 gcggcgcggc ggcccagggc aagtaccagg gccaggccac ggcagccctc aactggatga
     4141 agacccagca gcagccggat ggctccttcg cgggcttcgg cgcaggctcc accctggacg
     4201 cggtgctggc gatcatggcg gcggggcagt ccccctcgtc ctacagcatc gacggcaaca
     4261 cgcccataac gtttcttgag agcaaagtgg ccgacctcac caggaacccc ggcagcgcgg
     4321 gcaagctgct aatcgtgacg ggcaacctca acggcaacgg caccttcggg ggcgtggacc
     4381 tcgtgggcct catcaacagc agctacgatc ccgctacagg ccattacggt ggggatgccc
     4441 tcggccacgc gttcgccatg ctcggcctgc acgccgccgg gcaaaccata ccccctgcgg
     4501 cagtggagta cctcaaaggc acgcagaccc cggagggcgg ctgggcgttc acgggcgaca
     4561 ccgcaccggg cgctgccgac accaacacca cggcggtcgt catccaggct ttgcaggcgg
     4621 cgggcgcgac caatacccag cccgacctgc tcaagaaggc cgtcacctac atcgactcgc
     4681 agcagaaccc cgacggcggc ttcccctacc agaaggtggg cggccccgac agcgagagcg
     4741 acgtgaactc cagctcctac gtcgcgcagg ccatgctcag cctgggcaac accacgatag
     4801 ccgaccgggc gctagccttc atcgcctccc tacagaagcc ggacggcgcg ttccagtgga
     4861 agccgtcgga gcctgacgac accgccgggg ccacctacca ggctgtgtcc gcgctactac
     4921 aaacgaccct ggtaacgccg ctgcccggca ccgcatcgca gggtgaaaca agcagcccgg
     4981 tgcccggcag gcccacgacc ggcagcccgg taggcgattt gctgggtaca ctggccgcgc
     5041 tcgctgccct ggcgctgagt gcgggcgtgc tggtgcggcg tggcgcacag ggcaggtaag
     5101 tacaaaccgc accaggcagg aattgatgcg acgcagctac acgacagggt acggcgtgac
     5161 acgcccctac acgaccagta ggggcatgcc tcaccgtgcc ctgtcgctcc tgctggtcat
     5221 agtgtgcgtg gggctggcgg ggggaagcgc cgccggggca ggtagcgcgc agagtgagag
     5281 ccacgccggg ctggtcgtcc agttcgcgga cggcactacc aacacctact gcctggcgtt
     5341 caccggcgaa tcgatcaccg ggttggacct gctactcaag accggactcg acgttacagc
     5401 cgaaacgcag ggggcgcttg gcggctggat ttgtaagatc ggccccgacg ggtgcaactt
     5461 cccggaacag ccatgcgcgt gccagtcgta cgtcctcggc ggcaagtact gggtctacag
     5521 ccatctcaag gacggcgtgt ggaagacctc gcagctgggg gccagcaact acaaggtgcg
     5581 cgacggcgag gttgagggtt gggcctggag caccggcaag gggccgagcg tcacgcccag
     5641 cttcgcagag ttgtgcgccg ccgtgctgcc cgcgcccccc gagcctaccg ccaccaaccc
     5701 accgccccca cccccaccaa caaacacccc cctgccgccc ctccccaccg atacccccgt
     5761 gcctcccacc agcacgcccc agccgcctgc ccccacagcc acacccagac cgcgcctcac
     5821 ccccacgcct gcccccgcgc cacctaccgc cgaggtaccc cagcagctag cgcccactcc
     5881 cacacaagag ccgcccaccc agacacccac aacgctgccc accgatacgc ctgtgccaac
     5941 agacacgccg acgacgctgc caac
//
LOCUS       DASRWZ010000761         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_130118, whole genome shotgun sequence.
ACCESSION   DASRWZ010000761 DASRWZ010000000
VERSION     DASRWZ010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130118"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1709)
                     /locus_tag="VFH60_12085"
                     /pseudo
     CDS             complement(54..1709)
                     /locus_tag="VFH60_12085"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
     gene            2092..3936
                     /gene="glmS"
                     /locus_tag="VFH60_12090"
     CDS             2092..3936
                     /gene="glmS"
                     /locus_tag="VFH60_12090"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET6314565.1"
                     /translation="MCGIVGYVGPREATGIIVEGLCNLEYRGYDSAGIAVQNNGSIER
                     RRAAGKLSNLQALLRDEPITGHRGIGHTRWATHGKVNEANAHPHRDCSGDIVVIQNGI
                     VENYLELKETLGAQGHVFLSETDTEVLPHLIEQCLKDGAPSLQEAVRRMLLAIRGSHA
                     IVAMRAEEPDLLVAARLGNAGGVVIGYGEGEMFVASDQPAILQHTRRLAFLDPGEFAV
                     LTNDGARYYKLDGSEVSKKPQQFAWDPISAAKGGYKHFMLKEIHEQPRALMDTLRGRV
                     NAGTGHIDLDGVTLTAAEMRELKEVVVLACGTAFHAGLAGKYIIEELARIPVTVEYAS
                     EYRYRNPVVEPNTLAVIITQSGETVDTLEAMAEAHSHGARVLSIVNVIGSQADRISES
                     KIHVQAGPEVAVASTKVFTGMIADLVLLGMLLGQARGTLDDARSRELADALLRLPNQA
                     ADLLADAALYDELAGRLHNASDMLYLGRGVNYPTALEGALKIKEISYIHAEGCPAGEM
                     KHGINALIDNKLPVVCIALQDHVYSKMLSTMEQVKARDGIVVAIATEGDEAIREIADE
                     VIYVPKVDYLLSPILAVMPLQMLAYKVAVRRGADVDQPRNLAKSVTVE"
BASE COUNT          852 a         1260 c         1265 g          661 t
ORIGIN      
        1 aacacaaccc caaggtggtt tcggttctac aatttcaaat attggttttt agctcagttg
       61 gccgacacac cggcgaaggg gcctccggtc gattcacccg tctttgcagc cgtgttacaa
      121 catagtacgc ccgcgcgttg cagttccttc agccgggtgt acgcctccca gttggaaagg
      181 tggaacgcct gccagtttcg ccagctcttg ttggtctcca gcctcttcaa tcgtctttcc
      241 aggtcgttga tgatgtccat gcgcgccagc ccttctgcgt cgcgcagccc gtcggccagc
      301 accggcacag catcgttcga cagcacgtac aggtagcggg tcgccaggtc gtcgccccgg
      361 tgcgcgaggt tataggcggc tacttcagca tcggggttca gtatgttgat ggtcatcagg
      421 aacaccagca gtcccacgaa ggccccaatc gcgaacctct ccggcttcac ccacagggta
      481 gctgccagcc acaagaatgc caaccccagg aaaaccacga acacccgcac gaagatgcgc
      541 aggggcgtgt tgatgtagaa ctcgatgttc tcccacacca gcatgcgcca gaacgccgag
      601 acgagcagca ccagcgtcag cccgaccagg gccgacgaca gcaggttgta cacacgcacg
      661 tgccggtccg tgacgagccg ggccgcccag cgcagcccga gtatcagcgc catcgtgagc
      721 atcgacacga agagtagctc gccgaagccc tgccgcacgt actccctgta ggccgcgtag
      781 ttcatggtgc gttcggcgtg gccgctgaac aggtaggcga actgcaccca ggcgaaggcg
      841 gcaaagagca cgttgaccat ccccagcacc gtggcgctct cgccaaaccc tatccgccgc
      901 ttcggcttga agtcacccgg tagcgcgtaa agctcccgca cgtcctcggc ctcgcgctag
      961 cggctgagcg agaagtatat agcgcccgcg caaagccagg caacggtgag aatgaaggcc
     1021 acgtgctgca tcgtttcggg caggctcgag gcgaacttca tcgcgaacac ttcgcgcagg
     1081 tagcgggcaa agaaaatatc ggcagacgag agcagcaagc taaagacgat cagtaccggc
     1141 agcgccaaca aaaggccgcg cagcagcggc accaggaggc gggcgcgctc gcggtgcgcc
     1201 ttcgcggagg aggctacctg catgacgggt ggcaccgggt cggccaggct gcgtcccagg
     1261 ctggtgaaga gcaccgccgg gtagccgagt atgcccaggc gctctacctg gtcggccgcc
     1321 aggaagtaga gcagcagccc catgagcagc accaccgccg ccaggttcat ggaggtgagc
     1381 gacgagttgg cccgcacgaa gagcatgccg gcgaagaaga gcagcggctc caacagccac
     1441 aggttgcgcc tcgccaggct cacacgctcg agcctgctca gtccgaagat cgagcctagc
     1501 aagagcaaca caaagagcag gacggagatt ccgatattct ggccgtagaa cagcagatcg
     1561 ctcgcccacc ccagccccag ggcaacccac aggaccctta gtggcgcctt cagcgtttgc
     1621 attgccaagc cccctcgcag gaagaaatgt tgtggagatg ctgctacatg gtgccaggcc
     1681 acccggcccg cagtaacagc ggcggacata ctccataacg ggcgagagtc actgcggttg
     1741 catgcgcgca cggcaagaga caacctgcaa gtgataatcg ggcaggagtc tgttatgggc
     1801 gctgtttgag gctgccggtg gtttttgtag cgcgagtagt tgctttagat gtactcctta
     1861 cccgtccccg gtaggcttcc ctggtcgtgg tcttgcattc acactggtgg cggtggttca
     1921 tcagccacgg ggccggtccg ccgctacgct cccgcccagg ctgtcggagg cgctgtggta
     1981 taatcacgca gggtctctta ccccaaaccg ggggtaaacg tcccctcaca acagacggtc
     2041 cgcgccgagc ggtgacactc gagcgggggc agacttggag cgccgtaacg catgtgtgga
     2101 atagtaggtt acgtaggccc gcgagaggcc acgggtataa tcgtcgaggg cctgtgcaac
     2161 ctcgagtacc ggggctacga ctcggcaggt atcgcggtgc agaacaatgg ctcgattgaa
     2221 cggcgaagag cggcgggtaa gctgagcaac ctgcaagccc tgttgcgcga cgagcctata
     2281 acggggcaca gaggcatagg acacacccgc tgggccaccc acggcaaggt gaacgaggcc
     2341 aacgcccacc cccaccgtga ctgttccggc gacatcgtgg tcatccagaa cggcatcgtg
     2401 gagaactacc tcgaactaaa ggagacactc ggcgcgcagg ggcacgtgtt cctgtctgag
     2461 accgacaccg aagtgctccc ccacctcatc gagcagtgcc tcaaagatgg cgcacccagc
     2521 ctgcaagaag ccgtacgccg tatgttgctg gccatacgtg gctcgcacgc catcgtcgcc
     2581 atgcgcgccg aggagcctga cttgctggta gccgcccgcc tgggcaacgc aggaggcgtg
     2641 gtgattggct acggcgaggg cgagatgttc gtcgcctccg accagcccgc catcctgcaa
     2701 cacacccgga ggctggcctt cctcgaccct ggcgagttcg cggtgctcac gaacgacggc
     2761 gcgcgctatt acaagctcga cggcagtgaa gtaagcaaga agccgcagca gttcgcctgg
     2821 gaccccatca gcgcggccaa gggtggctac aagcacttca tgctcaagga gatccacgag
     2881 cagccgcgcg cgcttatgga cacgctgcgt ggccgagtga acgccggcac cgggcatatc
     2941 gacctcgatg gcgtgacgct gacggcagct gagatgcgcg agctaaagga ggtcgtggta
     3001 ctggcttgcg gcacggcttt ccacgcgggc ctggcgggca agtacattat cgaggaactt
     3061 gcccgcatcc ctgtcaccgt ggagtacgct tcggagtatc gctatcggaa cccggtagtc
     3121 gagccgaaca cgctggccgt tatcataacc cagtcaggcg agacggtgga caccctggag
     3181 gcaatggcgg aggcccacag ccacggggcg cgggtgctct ccatcgtgaa cgtgataggc
     3241 agccaggccg accgcatctc cgaaagcaag atacacgtgc aggccggccc ggaggtggcg
     3301 gtcgccagca ccaaggtctt cacgggcatg attgcggacc tggtgttgtt gggcatgctg
     3361 ctggggcagg cgcggggcac gctggatgac gctcgctcgc gggagttggc tgacgctctc
     3421 ctcaggctgc ccaaccaggc cgccgacctc ctggcggacg cagccctgta cgacgagctt
     3481 gcaggtaggc tgcacaacgc gagcgacatg ctctacctgg gccgcggcgt aaactacccc
     3541 acggcgttgg agggtgcgct caagatcaag gagatcagct acatccacgc cgagggctgc
     3601 cccgccgggg agatgaagca cggtataaac gcgctcatcg acaacaaatt gccagtggta
     3661 tgcatcgcct tgcaggacca cgtctacagc aaaatgctca gcaccatgga gcaagtgaag
     3721 gcccgagacg gcatcgtggt agccatcgcc accgaaggcg acgaggccat acgggagata
     3781 gccgacgagg taatttacgt gccgaaggtg gactacctgt tgtcccccat actggctgtc
     3841 atgccgctac aaatgctggc ctacaaggta gccgtgcgtc gcggcgccga cgtcgaccag
     3901 ccccgcaacc tcgcaaagtc ggttacggta gagtagccgt gcgttcgagg cacgggcgtt
     3961 cttacattgt tactgagtgc cgcagttttg aaagtacgtg cggaatagcg gcgacaagcc
     4021 agtgcgcccc aagttaga
//
LOCUS       DASRWZ010000762         7629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1295237, whole genome shotgun sequence.
ACCESSION   DASRWZ010000762 DASRWZ010000000
VERSION     DASRWZ010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295237"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1035)
                     /gene="argF"
                     /locus_tag="VFH60_12095"
     CDS             complement(103..1035)
                     /gene="argF"
                     /locus_tag="VFH60_12095"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET6314566.1"
                     /translation="MAVSMKGRNFVSVADFSTEEIWNVWQLAAQLKSKVKAGEPHRLL
                     EGKQLAMIFQKPSLRTQVSFETGMSQLGGHAIFLGPDHIQLGKRESVPDVARVLGRMV
                     DGIMARVFAHRDVEELAEHAGVPVINGLSDAEHPCQALGDLFTIYEKKRRIEGVNLTY
                     IGDGNNVAASLLLAAARVGMNITLISPDGYEVPSHYVQRAKTDAASTGARLQFSSDPD
                     DVVGSDVVYTDVWASMGQEAEAEQRARIFRPYQVNEALLRQSGGQPLVMHCLPAHRGH
                     EITDAAMDGPNSVVFEQAENRLHVQKAIMALLIQ"
     gene            complement(1154..2158)
                     /gene="arcC"
                     /locus_tag="VFH60_12100"
     CDS             complement(1154..2158)
                     /gene="arcC"
                     /locus_tag="VFH60_12100"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET6314567.1"
                     /translation="MSDTAPAQGRTYIETLVIALGGNAIQQAGQKGTAAEQFANVSHA
                     MRSVADLADRGYRVVLTHGNGPQVGTILLQQAAGEEHHGIPAVPMDMAGAMSQGQIGY
                     MMQQCLQNELRSRGRPWPVACVVTQTVVDPADPAFKNPTKPIGPFYTAERAEELRAKG
                     YTVIEDSGRGYRRVVPSPQPAAIAEIYAIRTLVNSGALVICSGGGGIPVVRDEQGLLH
                     GVEAVIDKDMSASLLAQRLDADRLLILTDVEQVYINYRKPDQQALSRLSLAEAQGYIQ
                     QGHFAAGSMGPKVRAAVGFVEAGGREAIITHLHSVGDALEGKTGTRIVSTEFQVPSSE
                     "
     gene            2481..4310
                     /locus_tag="VFH60_12105"
     CDS             2481..4310
                     /locus_tag="VFH60_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET6314568.1"
                     /translation="MFAERRDEISRRWNAVANVRLGAFVLAVACLGWGFWQDNGLVKI
                     AGLLLLADFFVLVRYHNLLGRQRRRYDELYNINNEARLRLHRQWTALPLRHSILPDPA
                     SPYSFDLDIFGRASLFQLVETVSTSMGEATLGSWLKRFAPVEVVRKRQAAVAELAPMI
                     DLRDELALPGRLSQDKPDPEPFLKWAEGEPWLAKRAWLRWGAIADVALLWVLLLAHFF
                     GWIEYPLYLVLAAINLLAGLTIVKTVYTIMATASTREKAFAQYATMVELLSRQGFASP
                     LLREMQARLLVDGEHAHALMRKLHRLTTLTIPPSAQLYIVIQAFTLWDVHLLAAFEGW
                     QAKAGKHAREWLDVLGEAEALSALSVLAHDNPGWTFPDVDGEARSLEARQLGHPLIAP
                     GVRVDNDVQVGPEGTFLLVTGSNMSGKSTLLRAIGVNIVLAGAGGPVCARWLRLPPVE
                     LWTSMRVQDSLEEGVSYFMAELKRLKQVVDAGRRLSGGERRLFYLLDEILHGTNTAER
                     QIAARRVVMYLVEQGALGAVSTHDLTFADSPTVAAAARMFHFTETFSNGPGGPQMSFT
                     YKLTPGIATSTNALKLMQIVGLDLGGGGGLVGDGNAKTPSTPR"
     gene            complement(4410..4937)
                     /locus_tag="VFH60_12110"
     CDS             complement(4410..4937)
                     /locus_tag="VFH60_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314569.1"
                     /translation="MPLLTIELSKEKLQALEQEARALGMTPQRRAAEIVEQAVTASAS
                     KGQKKRAEGLQKLQAYMERIPAVQVVSASEASEARWWLKFDIDIHHPLAWNVVQELGF
                     VLNYLSLTERLPTVFKPVSPPPYMNGGPADFLSWVIEAEEPLPYAGYIAEVLEGRLPR
                     PVDDETQWDTSDDEA"
     gene            complement(4966..5385)
                     /locus_tag="VFH60_12115"
     CDS             complement(4966..5385)
                     /locus_tag="VFH60_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET6314570.1"
                     /translation="MAEIFVDTAGWAQLFVPSEPYQIAAASIYTQALQRGSTLVTTNY
                     VITELVALLTSPLRVPRRTIISLVGDLKVASHVEIVHVDPTLHEQAWLLFTKRPDKNW
                     SLVDCASFVVMQQRSIQEALTTDHHFEQAGFTNLLKP"
     gene            complement(5399..5647)
                     /locus_tag="VFH60_12120"
     CDS             complement(5399..5647)
                     /locus_tag="VFH60_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314571.1"
                     /translation="MAHTLLLELPDDVYKLLAQTAEQTSQPLEELAVEWLTNATRRVT
                     EDPLAKYIGAIHTDVPNWGERHDELLGQALMDDHRSEE"
     gene            complement(5694..7445)
                     /locus_tag="VFH60_12125"
     CDS             complement(5694..7445)
                     /locus_tag="VFH60_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6314572.1"
                     /translation="MNGNGKDNSLVSQLLTRVEAERNGSHTAERTLLGQVTAADTERG
                     VTIHLRREQPVELLRQGQFVVVEGQHLRFFGTIGAFRLATTDPAVGMDPPDSSSPLIR
                     DTLSRSTTYAECNVRLALEMVEGSVRPARTIPGPFSQSYTAGQQDYQTVFGTEDRHHF
                     IIGSPRELDESLAAEAPICIDLRKFVERSNGIFGRSGTGKSMLARLLLCGLVKSEAAV
                     NLIFDMHSEYATEKELEPRGTGKYAKGLRDLFGKSRVALYTMDEEDSRKRGGADGVIR
                     IALSDITIDDIEALGSELKMSEGSAMLITARQAYRKMGRNWIERLVSMGPGELEAYCT
                     ETGANSGSVEALKRRLENIADFSFVVDSKQAVGTGIDGLIKDLRAGRHVILMFGKFRS
                     TLAYMLVANILTKRIHDEWVRWSERHEQDREGTEAPRPLMITIEEAHKFLGRDSTTIF
                     GTIAREMRKYLVTLMVIDQRPSGIDDEVMSQIGTRVTALLNDDKDIDAVFMGIRGGGQ
                     LKALLATLDSKQEVMVMGHAVPMEVVLRVRSFDADFYKSLTTTGPSTTPSQARTVSEN
                     IVKSEQKKASLEDLFGE"
BASE COUNT         1263 a         2298 c         2516 g         1552 t
ORIGIN      
        1 ggagtgatga ggactgagga ctgaggactg agtaactagt aatgtggact gaggactgat
       61 taggtccaga acgaacactc agtcctcagt cctcattact cgttactgaa taagcagggc
      121 catgatcgcc ttctggacgt ggaggcgatt ttcggcctgc tcgaagacta ccgagttggg
      181 gccgtccatc gcggcgtcgg tgatttcgtg gccgcggtgg gcgggcaggc agtgcatgac
      241 gaggggctgg ccgccgctct gcctgaggag ggcttcgttc acctggtagg ggcggaagat
      301 gcgggcgcgc tgctcggcct cggcctcctg gcccatgctg gcccacacgt cggtgtagac
      361 cacgtcgctg cccaccacgt cgtcggggtc gctagagaat tgcaggcgcg cgcccgtact
      421 ggcggcgtca gtcttagccc gctgcacgta gtgggaaggc acctcgtagc cgtcggggct
      481 gatgagggtg atgttcatgc ccacgcgggc cgccgccagc agcagcgagg ccgccacgtt
      541 gttgccgtcg cctatgtagg tgaggttgac cccttcgatg cggcgcttct tctcgtagat
      601 agtgaagagg tcgcccagcg cctggcaggg gtgctccgcg tcggacaggc cgttgatcac
      661 gggcaccccg gcgtgctcgg caagctcctc cacgtcgcgg tgggcgaaca cgcgggccat
      721 gatgccgtca accatgcggc cgagcacgcg ggccacgtcg ggcacgctct cgcgcttgcc
      781 aagctggatg tgatcggggc cgaggaagat ggcgtggccg ccaagctggg acatgccggt
      841 ctcgaagctc acctgagtgc gcaacgacgg cttctggaag atcatggcaa gctgcttccc
      901 ctccaacagg cggtgtggct ccccggcctt caccttgctc ttgagctgcg cggcaagctg
      961 ccacacgttc catatctcct cggtgctgaa gtcggccacc gacacgaaat tccgaccctt
     1021 catcgatact gccattgcgt tctccttctg acgagttccg agttccgagt ggtcagtggt
     1081 tagtggttag tggcacacac cccaaacctt catactcttt gcgcgtgctt tgcgtctttg
     1141 cgcttgtgtc gccctactcg gaactcggaa cttggaactc ggtacttact atcctcgtgc
     1201 ctgttttgcc ttccagcgca tctcctacgc tgtgcaggtg ggtgatgata gcttcgcggc
     1261 cgcccgcctc tacgaagccc accgccgccc ggaccttggg ccccatgctc ccggcggcga
     1321 agtggccttg ctggatgtag ccctgggctt cggccaggct caggcgggag agggcttgct
     1381 ggtcgggctt gcggtagttg atatagacct gctccacgtc ggtgaggatg agcaggcggt
     1441 cggcgtcgag gcgttgggcg agcagggagg cgctcatgtc tttgtctata accgcctcca
     1501 cgccgtgcag caggccctgt tcgtcccgca ccacgggtat gccgccgccg cctgagcata
     1561 tcaccagcgc gccgctgttc accagcgtgc gtatggcgta gatttcggct atcgcggcgg
     1621 gctgcggaga gggcaccacg cggcggtagc ccctgccgct gtcctcgatc acggtgtagc
     1681 ccttcgcgcg tagctcctcg gcccgttcgg ccgtgtagaa gggccctatc ggcttggtgg
     1741 ggttcttgaa ggcggggtcg gcggggtcca ccaccgtttg cgtaaccacg caggcgacgg
     1801 gccacggcct gccccggctc cgcagctcgt tctggaggca ctgctgcatc atgtagccaa
     1861 tctgcccctg gctcatcgcc ccggccatgt ccatcggcac ggccggtatg ccgtggtgct
     1921 cctcgccggc cgcctgttgg agcaagatag tgcccacctg ggggccgttg ccgtgcgtga
     1981 gcaccacccg gtagcccctg tcggccaggt cggccaccga gcgcatggcg tggctgacgt
     2041 tggcgaactg ctcggcggct gtgcccttct gccctgcctg ctggatggcg ttacccccca
     2101 gcgcgatcac gagcgtctcg atgtaagtcc ggccctgcgc cggggcggta tcggacaacg
     2161 ctgtccctcc catctagcaa gtctggtgtc gcgacatggc cgctccctta tgttagcacc
     2221 caggccacaa atcgaaaaga gggggcaacc aaaccggctg cccctcgcgt gcgacattat
     2281 aacatagttc ggcacgggca ggcttggcgg tcccgtctat acacatgccg ggcgttgggg
     2341 cgcgttctga tgttatactc aattattcct tttgaaagag gcatagcatc gacgcgcaga
     2401 ctatcgagca gccaatcgtc cggattgaag cgggaagccg cgaccggccg gcccaggaag
     2461 tatatgacgc ccgctgtacc gtgttcgcgg agcgcaggga cgagatttcg cgccggtgga
     2521 acgccgtcgc caacgtgcgg ctgggggcgt tcgtgctggc ggtggcgtgc ctggggtggg
     2581 gcttctggca ggataacggg ctggtcaaga tagcggggct gttgctgctg gccgacttct
     2641 tcgtgctggt gcgctaccac aatctgctcg gcaggcagcg gcggcgctac gacgagctat
     2701 acaacatcaa caacgaggcc cgcctgcgcc tgcaccggca gtggaccgcc ctgcccctgc
     2761 ggcactcgat actgcccgac ccggccagcc cttactcgtt cgacctggac atctttggcc
     2821 gcgcctccct cttccaactg gtagagacgg taagcaccag catgggcgag gccacgctgg
     2881 gaagctggct gaagcggttc gccccggtgg aagtagtgcg caagcggcag gcggcggtag
     2941 ccgagcttgc ccccatgatc gacctgcggg acgagcttgc cttgccgggc aggttgtctc
     3001 aggacaagcc ggacccggag ccgttcctga agtgggcgga gggcgagccc tggctggcga
     3061 agcgcgcatg gttgcggtgg ggcgcgattg ccgatgtagc gctgctctgg gtgctcttgc
     3121 tcgcccattt cttcggctgg atagagtacc cgctctacct cgtgcttgcc gctatcaact
     3181 tgctggcggg gctgaccatc gtcaagacgg tctacacgat catggctacg gcttccacgc
     3241 gggagaaggc gttcgcccag tacgcgacga tggtagagct gctgtcgaga cagggttttg
     3301 ccagcccgct gctgcgggag atgcaggccc gtctgctggt agacggggag catgcacacg
     3361 cgctgatgcg caagctgcac cggctcacca cgctcacgat acccccttcg gcgcagctat
     3421 acattgtgat acaggcgttc accctgtggg acgtgcacct gctggcggca ttcgaggggt
     3481 ggcaggcgaa ggcaggcaag cacgcccgcg aatggctcga cgtgctgggg gaggcggagg
     3541 ccctatcggc cctgtcggtg ctggcgcacg ataaccccgg ctggaccttt cccgacgtgg
     3601 acggcgaggc gcggtcgctg gaagcgaggc agttagggca cccactgatt gcccccggcg
     3661 tgcgcgtaga caacgacgtg caggtggggc cggagggcac tttcctgctg gtgacaggct
     3721 cgaacatgtc gggcaagagc accctgctga gggctatcgg ggtgaacatc gtgctggcgg
     3781 gagcgggcgg gccggtgtgc gcccggtggc tcaggctgcc gccggtggag ctttggacga
     3841 gcatgcgcgt gcaggactcg ctggaggagg gcgtgagcta cttcatggcg gagctgaagc
     3901 ggctcaagca ggtggtggac gccgggagga ggctatcggg cggggagagg cggctcttct
     3961 acctgctgga cgaaatcctg cacggcacca acacggcgga gcgccagata gccgcccgcc
     4021 gcgtggtgat gtacctggtg gagcaggggg cgcttggggc cgtctccacc cacgacctga
     4081 ccttcgcgga cagccccaca gtggcagcgg cggcccgcat gttccatttc accgagacgt
     4141 tcagcaacgg ccccggcggc ccgcaaatga gcttcaccta caagctgacg ccgggcatcg
     4201 ctacgtctac caacgccctc aagctgatgc agatcgtggg gctggacctg ggtggtgggg
     4261 gtggcttggt tggagacgga aacgccaaga cgccaagcac gccaaggtag cgccaagatt
     4321 aagaagggtt tgcagcgttc agaaaaggtc agggtacaaa ggcacgtatc ccggcctttt
     4381 tgcgttcctc gaacctacat cctcagagac taagcctcgt cgtccgatgt atcccactgc
     4441 gtttcatcgt ctacagggcg gggcaggcga ccttccaaca cttctgcgat gtagcctgca
     4501 taggggaggg gttcttcggc ttctatcacc caggatagga agtctgccgg gcctccgttc
     4561 atgtaaggag gtggggagac gggcttgaac acagtgggaa ggcgctcagt caaggacagg
     4621 tagttcagca cgaagcctag ttcctgcacg acgttccagg cgagtgggtg gtgtatgtct
     4681 atgtcgaact tgagccacca gcgggcttcg gaggcttcgc tcgctgagac gacctggact
     4741 gcgggtatcc gctccatgta cgcttgtagc ttttgcaggc cctcagctcg ttttttctgg
     4801 cctttgcttg ccgatgctgt gactgcctgc tctactattt cggcggcgcg ccgctggggg
     4861 gtcatgccta gtgcgcgggc ctcttgctcc agggcttgta gcttctcttt agaaagctct
     4921 attgtcagca ggggcatagc gggttccggt agttggcaaa caaatttacg gctttagcaa
     4981 gttcgtgaag cctgcttgct caaagtggtg gtctgtcgtc aaagcctctt gtatgcttcg
     5041 ctgctgcatt acgacaaagc tggcacaatc gaccaggctc cagttcttgt cagggcgctt
     5101 tgtaaatagc agccaggctt gttcgtgaag cgttgggtct acgtgcacaa tctcaacatg
     5161 tgatgccacc ttgaggtctc ccactagcga aataatggtt cgtcttggga ctcggagggg
     5221 actagtgaga agtgccacca gttctgtgat gacatagttc gttgtgacaa gggtacttcc
     5281 gcgctgtaga gcctgcgtgt agatgctggc ggcggctatc tggtaaggtt ccgacgggac
     5341 gaataactgc gcccagccag cagtatcaac gaagatctca gccatctagc ccgtttctct
     5401 actcttcact tctatggtca tccattaacg cctgacccag aagttcgtcg tgacgctcgc
     5461 cccagttagg tacatcagtg tgtattgcgc caatatactt cgcgagggga tcttctgtga
     5521 cacgccgcgt cgcgttggtc aaccattcaa ccgcaagctc ttccagtggt tggctggtct
     5581 gctcggctgt ttgcgctagt aacttgtaca cgtcgtcggg tagttccagc agtaatgtgt
     5641 gagccatatg tcatactcct atcactcacc catagtatat catgaccaac ttcttactcc
     5701 ccgaacaagt cctccaagct cgccttcttc tgctcgctct tgacgatatt ctcgctcacc
     5761 gtgcgggcct ggctgggggt cgtggagggg cccgtggtgg tgaggctctt gtagaaatcg
     5821 gcgtcgaagg agcggacgcg cagcactacc tccatcggca cggcgtggcc catgaccatc
     5881 acctcttgct tgctgtcaag ggtggcgagg agggccttta gctgcccgcc gccccggatg
     5941 cccatgaaca ccgcgtctat gtccttgtcg tcgttgagca gggcggtgac gcgggtgcct
     6001 atctgcgaca tcacctcgtc gtctatgcct gaggggcgct ggtcgattac catgagggtg
     6061 acgaggtact tgcgcatctc gcgggctata gtgccgaaga tggtcgtgct gtcgcggccc
     6121 aggaacttgt gggcctcctc tatggtaatc atgagggggc ggggggcttc ggtgccctcg
     6181 cggtcctgct cgtgccgctc ggaccacctg acccactcgt cgtggatgcg cttggtgagg
     6241 atgttggcta ccagcatgta ggccagggtg ctgcggaact tgccgaacat gaggattacg
     6301 tgcctgcccg cccgcaagtc cttgatcagg ccgtcgatgc cggtgcccac cgcttgcttg
     6361 gagtcgacca cgaaggagaa gtcagcgatg ttttccaggc ggcgcttgag ggcctccaca
     6421 gagccactgt tggcccccgt ttcggtgcag tacgcctcca gctcgccagg ccccatgctc
     6481 accagccgct ctatccagtt gcggcccatc ttgcggtatg cctggcgggc cgtgatgagc
     6541 atggccgagc cttcgctcat cttcaattcg ctgcccaggg cctctatgtc gtcaatcgtg
     6601 atatcgctca gggcgatgcg gattaccccg tcggccccgc cacgcttgcg gctgtcctcc
     6661 tcgtccatcg tgtagagggc cacgcgggac ttgccgaaca ggtcgcgcag gcccttggcg
     6721 tacttgccgg tgcctctcgg ctccagctcc ttctcggtgg cgtactcgga atgcatatcg
     6781 aagatgaggt tgaccgccgc ctcggacttg acgaggccgc acaggaggag gcgggccagc
     6841 atgctcttgc cggtgcccga ccgcccgaag atgccgttgg agcgctccac gaacttgcgc
     6901 aggtcgatgc agataggggc ttcggcggcc aggctctcgt caagctcgcg gggggagcct
     6961 atgatgaaat ggtgccggtc ctcggtgccg aacacggtct ggtagtcctg ctggcccgcg
     7021 gtgtaggact ggctgaaggg gccggggatg gtgcgggcgg ggcgcacgct gccctccacc
     7081 atctccaggg ccaggcgcac gttgcactcg gcgtaggtgg tggagcggga gagggtgtcg
     7141 cgtatcaggg gagaggagga gtcgggcggg tccatgccga cggcggggtc ggtggtcgcc
     7201 agcctgaacg cgccaatcgt accgaagaag cgcagatgct gcccctcgac caccacgaac
     7261 tgcccctggc gcagtagctc gaccggctgc tcgcggcgca ggtggatagt aacgccgcgc
     7321 tcggtgtcgg cggccgtgac ctggccgagg agggtgcgct cggcggtgtg actgccgttg
     7381 cgttcggcct ctacgcgggt gaggagttga gataccaggc tgttgtcttt gccgttgccg
     7441 ttcatgcggt gcgtcctctc aagtcacttt caagcaacta taaaatgtaa agaaggggag
     7501 aactggggga cacccccggc cccgtcaggg gaggacccct gcaccccctt gtgccctaac
     7561 gacggtagcg ggtgccctct gccgagcggt ccttgttcag gcggcggttg aggtgcaggc
     7621 cctccagga
//
LOCUS       DASRWZ010000763         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1226851, whole genome shotgun sequence.
ACCESSION   DASRWZ010000763 DASRWZ010000000
VERSION     DASRWZ010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226851"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(523..1500)
                     /locus_tag="VFH60_12130"
     CDS             complement(523..1500)
                     /locus_tag="VFH60_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET6314573.1"
                     /translation="MPKPKGGLGRGLDSLIPSSPQASTDAQTQPAPTTGAPASTGTGG
                     LATEAGVLMLPVDSIAPNPRQPRGAFDLEGLEDLAASIREHGVLQPLVVTMSGGAGGA
                     SSYNLIAGERRWRASMLAGLEQVPVIVKEATSRGMLELALVENLQRADLNPLEEATAY
                     RALVDEFDMKQDEVADRVGKSRQAVANSLRLLRLPPAILDALAAGTISEGHARALLQV
                     PEAEAQLQLAQRIASEGLSVRQVEAFARRLAEQAANEENAPDDETEEGRSDSLYDPIR
                     DLEEQFRTALGTKVQLSRSRRGGRLVIYFYSDEELERIYGTIIRDEPEG"
     gene            complement(1487..2308)
                     /locus_tag="VFH60_12135"
     CDS             complement(1487..2308)
                     /locus_tag="VFH60_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET6314574.1"
                     /translation="MYCFANQKGGVGKTTTAVNVAAYLAAGGHRVLLVDADSQANATS
                     SLGIDKHGEGPSLYDVLEGSAPASEAVLHVDRPPFRGGLYLLPSSVDLAGIEVELAGQ
                     EQGQREYRLRTALEPLRGDFDFMLIDCPPSLGLLTLNALTAADAVIIALQCEYLALEG
                     LMQLTSTISLVLRGLNKDLYILGIVMTMYDSRTNLSAQVVQEVKSHYPNEAFETLIPR
                     SVRLSEAPSYGLTILEYDPNSRGALAYAALSEELAARSGAHAAPVAEEVSSDAKA"
BASE COUNT          448 a          762 c          831 g          503 t
ORIGIN      
        1 gtggtgattg ccacgataat tgttggcttc ctggcttgtg cgatgctggg gttactggtg
       61 gaggccaaca actattctta ttagcggccg gtagaggaca gcgggcaata ttgacgccct
      121 gcatgacgcc ctgtggctga agccactggc tgagaagtag cgaagtctcc ttcggagact
      181 ggggcttctc tgcacagtgc tgcctgtgcg ggaccaaagt ggcccctgtc attctgagcg
      241 tagcgaagaa tctaaagaag caggtacagg gaggctattg aacatcacat cctgtggtgc
      301 tggcgttcaa cttagaggaa aggccagcat cttggattct tcgctacgct cagaatgaca
      361 aggtccatcg ttgttcgttt agttcctgct cttttgtaga tagaccctag cctcatgcac
      421 taggtagtct ccgaaggaga cttcgctact tctcagcccc tggctttagc cacagggcgt
      481 ccccacaggg cacctaagcg aggcataacg aaccagaagg cgttacccct ccggctcgtc
      541 gcggatgatg gtgccgtaga tgcgctcaag ctcttcgtcg gagtagaagt agatgaccag
      601 cctcccgccc cggcggctcc tggagagttg cactttggtg cccagggcgg tacggaactg
      661 ctcttcgagg tcgcggatgg ggtcgtagag cgagtcggac cggccttctt cggtctcgtc
      721 gtcgggggcg ttttcctcgt tggcagcctg ttcggccaag cggcgggcga acgcctcgac
      781 ctgccgcacg ctcaggccct ccgaggctat gcgctgggcc aattgcagtt gcgcttcggc
      841 ctccggcacc tgtagcaggg cgcgggcgtg tccctcgctt atggtgcccg ccgccagcgc
      901 atccagtatg gcggggggca accttagcag acgtagtgag ttggcgaccg cctggcggct
      961 cttgcccacg cggtcggcta cctcgtcctg cttcatatcg aattcgtcca ccaaagcccg
     1021 gtaagcggtg gcctcttcga gcgggttgag gtcggcgcgc tgcaagttct ccaccagcgc
     1081 cagctcgagc atgcccctgg acgtggcctc tttgacgatg accggcacct gttccagccc
     1141 ggccagcatc gaggcccgcc agcgacgctc gcccgctatg aggttgtagc tactcgcgcc
     1201 acccgcgccc cccgacatgg tgaccaccag gggttgcagc acaccgtgct cgcggataga
     1261 ggcggccagg tcctcaaggc cctccaaatc gaaggcccca cgaggctggc gggggttggg
     1321 cgctatgctg tccaccggca gcatgagcac gcctgcttcg gtagccagtc cgcccgtgcc
     1381 cgttgaggca ggcgcgccgg ttgtcggggc aggctgagtc tgagcatcgg tgctcgcttg
     1441 cggcgacgac gggatgaggg agtcgaggcc gcggcccagc ccgcctttag gctttggcat
     1501 cgctgctcac ctcctccgcg accggcgcgg catgggcacc gctgcgggcg gccagttctt
     1561 ccgagagggc ggcgtaggcg agcgcgcccc tggagttggg gtcgtactca agtatggtaa
     1621 ggccgtagct gggggcctcg ctcaggcgca cgctgcgggg tatgagcgtc tcaaacgcct
     1681 cgttggggta gtggctcttc acttcctgca ccacctgggc cgatagattg gtccggctat
     1741 cgtacattgt catcactata ccgagtatgt agaggtcttt gttgaggccg cgcagcacca
     1801 gcgaaatagt gctggtaagc tgcatcagcc cttccagcgc caggtactcg cactgcaacg
     1861 cgattatcac cgcgtcggcg gcggtgaggg cgttgagcgt gagcagaccg agggacggcg
     1921 ggcagtctat cagcatgaaa tcgaagtcgc cgcgcagtgg ctccagggcc gtcctgagcc
     1981 ggtactcgcg ctgcccctgc tcctgcccgg caagctccac ctcgatgccc gccaggtcca
     2041 ccgaggaggg cagcaggtag aggccgccgc ggaagggtgg gcggtctacg tgaaggacgg
     2101 cctcggaggc gggggcgcta ccttccagca cgtcgtagag ggaaggcccc tcgccgtgct
     2161 tgtctatgcc gaggctggac gtggcgttgg cctgcgagtc ggcatccacc aggagcacgc
     2221 gatggccccc ggcggcgaga taagcggcaa cgttgacggc cgtggtggtc tttcccacgc
     2281 cgcctttctg gttggcaaag cagtaaatgc gcccctttcg agggcgagaa ttggcttcca
     2341 ctgaggggta ttatagcaca gccattggag aggggagacg aaaccgccaa gacgccaaga
     2401 acgcgaagaa cgcgcaaaga ggatgatggt ttaaatgaga gtaaaaggac caggatacga
     2461 tataacgtac cctggacctt ttgcttcgaa caaccccatc ctctatcttg gcgtggtcct
     2521 tggcgttctt ggcgtcttgg cggt
//
LOCUS       DASRWZ010000764         1886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_541565, whole genome shotgun sequence.
ACCESSION   DASRWZ010000764 DASRWZ010000000
VERSION     DASRWZ010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541565"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFH60_12140"
     CDS             <1..654
                     /locus_tag="VFH60_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314575.1"
                     /translation="CQRDVPTASPFYPYIYNLAYHGVIGGYACGSNPDEPCAPPDNYP
                     YYRPNANITRGQISKLVALSAGFEDTPSGQSFADVPSTHTFWRWIGQLSSRGLIGGYP
                     CGGPGEACSPDKLPYFRSTNNATRGQLAKIVANAAGIDDDPGDPIFADVPPGHTFYDY
                     INPLARRGVMSGYPCGGPGEACGSDSLPYFRSSHNVTRGQAAKIVANTFWLDDWLEP"
     gene            889..1695
                     /locus_tag="VFH60_12145"
     CDS             889..1695
                     /locus_tag="VFH60_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6314576.1"
                     /translation="MKVEGFAEVNGTRLYYEKAGAGHPLVLVNGFSLDTRMWDDQVTT
                     FARHYEVIRYDARGHGKSAAPGDRGFYHAGDLKGLLDYLGCSRASLLGLSWGAAVATE
                     FVLAYPQMAGALVVADPVLWGHRWSPEYEASLSGLWEVGRNEGVEAARSLWLSHPMFA
                     AALERPEVADRLVEIVSAYSGWHWTHDDPALLPDPPAAWRLEDIAVPTLAIIGERDVP
                     DFHAVADEMSKRIPGARKVVLPGVGHMSNMEDPASFNDIVLAFLSRIWPK"
BASE COUNT          372 a          618 c          570 g          326 t
ORIGIN      
        1 tgccagagag acgtgcccac cgccagcccc ttctatcctt acatctacaa tcttgcctac
       61 cacggcgtga ttggcggcta cgcttgcggt agcaacccgg acgagccgtg cgcgccgccc
      121 gacaactacc cctactaccg ccccaacgcc aacatcaccc gcggccagat atcaaagctc
      181 gtggccctct ccgcgggctt cgaggacacc cccagcggcc agagctttgc cgacgtgccg
      241 tcaacccata cgttctggcg gtggataggc cagttgagca gccgcggcct gatcggcggc
      301 tacccgtgtg gagggcctgg cgaagcgtgc agcccggaca agctgcccta cttccgctcc
      361 acaaacaacg ccactagagg ccagttggcg aagatagtag ctaacgcggc ggggatcgat
      421 gacgaccccg gcgacccaat cttcgccgac gtgccccccg gccatacttt ctacgactac
      481 atcaaccctc tggcgcggcg cggcgtgatg agcggctacc cctgcggcgg cccaggtgaa
      541 gcctgcggct cggacagcct gccctacttc cgctcctccc acaacgtgac caggggccag
      601 gcagccaaga tcgtcgccaa cactttctgg ctcgacgact ggctcgaacc atagggacac
      661 accaaccacg ccgataagac acaccctgaa cagccacgag catcctgcca acaggatgct
      721 cgcggcacgt gcggcggtgg gctgtccgag ttcatgaggg atagaggttc gaaacccgtt
      781 agccttggga acggcagatg atagttgacc cgaaatccgg gcccacacta gaatgtgcaa
      841 gaaggatgct aatagtgggt caatcacttt gggaacagtg cgggagttat gaaagtcgaa
      901 ggattcgcgg aagtcaacgg aacgcgcctc tactacgaga aagccggggc agggcacccg
      961 ctagtgctcg tgaatggctt ctccctcgat acgagaatgt gggacgacca ggtaactacg
     1021 tttgctcgcc actacgaggt gatacgctac gacgcacggg gccacggtaa gtccgccgcg
     1081 cccggtgacc ggggcttcta tcacgcgggc gatctcaagg gtctgctaga ctacctcggc
     1141 tgctcgcgag cctccctgct cggcttgtcg tggggggcgg ccgtcgccac cgagttcgtg
     1201 ctggcgtatc ctcagatggc gggcgcgctc gtcgtcgccg accccgtgct gtggggccac
     1261 cgctggtcgc ccgagtatga ggcatcgcta agcgggctgt gggaggtagg acggaatgaa
     1321 ggtgttgaag ccgccaggtc gctctggctg tctcacccga tgtttgcggc ggccctggag
     1381 agacccgaag tggcagatcg cctggtagaa atagtctccg cttactcggg ctggcactgg
     1441 acgcacgacg acccggccct gctcccggac ccgcccgccg cgtggcgatt agaggacata
     1501 gccgtaccta ccctcgccat aataggtgaa cgcgacgtgc ccgacttcca cgctgttgca
     1561 gatgaaatga gcaagcgcat ccctggtgcc cgcaaggtgg tattgccagg cgtcggccac
     1621 atgtcgaaca tggaggaccc cgccagcttc aacgatatcg tgctggcttt tctttccagg
     1681 atttggccta aatagagccc tcatcccgat tgtgacgcgc cgcccaaccc gggacgtcag
     1741 cgcacaggct catacgcacg ttacgcccca ccagcacctc acggcgctgg gtcgtcgcgc
     1801 cctccgcagg acgccgaggt ttcgtggagc gccctgttcc ccggtgcgtg gaccaagggg
     1861 aatcaacgaa atgcgacagt agttgg
//
LOCUS       DASRWZ010000765         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1226959, whole genome shotgun sequence.
ACCESSION   DASRWZ010000765 DASRWZ010000000
VERSION     DASRWZ010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226959"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFH60_12150"
     CDS             complement(<1..383)
                     /locus_tag="VFH60_12150"
                     /inference="COORDINATES: protein motif:HMM:NF016553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314577.1"
                     /translation="MTMPFGTLAATLEALEPVSGRTQMIGMLAEMLGQAGPDDIGQLV
                     YLSQGRLLPDFIQKEFGMNERLVFRSIAQAYGAPADTVAARFKQLGDVGLVAQEFAQA
                     GAAQMALAGDAGTAEGVPLPEEYPH"
     gene            complement(509..1327)
                     /locus_tag="VFH60_12155"
     CDS             complement(509..1327)
                     /locus_tag="VFH60_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6314578.1"
                     /translation="MSDQNPLDRPNTTNLGWRIQQLRVRRGLQQKTLARLANVDAAFL
                     NRMERGRSSRSRPKPETIHRLLDAMDATPAEREAVFHVEVPPLSEEEIQSCVSEIEAN
                     YERAPEPMVLVDDRWNRWYINELGRRMFALSDDEYRRCLGRTTLDAFVDPEQPLYSRY
                     SEEHRELQFAQRLIIFKTFFAAQQFDTWYLAVEDYVKRFPLGRRVWEHPESYVPPIFM
                     FSQEVTMTDPQGRLYMLAAQADILLKTPRFMLVQLRPRDEETRRRLAALHAVEA"
     gene            complement(1857..2633)
                     /locus_tag="VFH60_12160"
     CDS             complement(1857..2633)
                     /locus_tag="VFH60_12160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6314579.1"
                     /translation="MLVSPISPVVIGLVLAIIVLTFFVWRWAVRTPVSVPPDPSVQDE
                     GEQEQEQVQEPQPFVYAGIGASDVVGVGATDPASESWVELLARYLPANTRLVKLGRNG
                     ITLNDANKVEVPQAVAAQPDLVTLWNCVNDATRGIPLPLYTRDLRSALDRLTLDTEAH
                     VLLLNLPDLSLLVPASDSARRELIQGGIRQWNAAMSQAAADYGDRVTLVDLFPGSEQV
                     LIRPDFVSADRFHPSSAGYRWLADHVWSVIQREKLPGSGA"
     gene            complement(2667..2879)
                     /locus_tag="VFH60_12165"
     CDS             complement(2667..2879)
                     /locus_tag="VFH60_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314580.1"
                     /translation="MSQDVGRRSSRRAISWVVVGVGAILLLLVPFYLVFNAVAGSTQV
                     TVGGTWLILSAVVGMGLIAAGAALAD"
BASE COUNT          567 a          894 c          923 g          520 t
ORIGIN      
        1 atgtgggggt actcctccgg taacggcacg ccctcggcgg tgcctgcgtc accggcgagc
       61 gccatctgcg ccgccccagc ctgcgcgaac tcctgggcca cgaggcccac gtcgcctagc
      121 tgtttgaaac gagcggcaac cgtgtccgcg ggcgcgccgt aagcctgggc aatcgagcgg
      181 aagaccagcc gctcgttcat gccgaactct ttctggatga agtccggcag caggcggccc
      241 tggctgagat agacgagctg gccgatgtcg tccggcccgg cctggcccag catctcagcc
      301 agcatcccga tcatctgggt gcgcccgctt accggctcaa gcgcttcaag cgtggccgcg
      361 agggtgccaa aaggcatagt catgtcgtgt gggaactcct gaaagcggta gtgataagcc
      421 gttggggcct acagcgcgat tatataacgc tgccgtgggg aaggcaaatc acaccggcgc
      481 gcccgatgca gagggttgtg aagcccggtc aggcttcgac tgcgtgcagc gcggcgaggc
      541 ggcgtcgcgt ctcttcgtct cgcggcctta gctgcacgag catgaagcgg ggagtcttga
      601 ggaggatgtc agcctgggcc gcgagcatgt atagcctgcc ctgagggtcc gtcatcgtga
      661 cttcctggct gaacatgaag attggcggca cgtagctttc cgggtgctcc cataccctgc
      721 ggcccagggg aaacctcttg acgtagtcct cgaccgccag gtaccaggta tcaaactgtt
      781 gagcggcaaa gaaggtcttg aagataatca ggcgctgggc aaattggagc tcacggtgct
      841 cttcgctata ccttgaatag agcggctgtt cggggtcaac gaatgcatcg agggtggtcc
      901 ggcccaggca gcgcctgtac tcgtcgtccg acagcgcgaa catgcgccgc cctagctcat
      961 tgatgtacca ccggttccac ctgtcgtcca cgagcaccat aggctcgggg gcgcgttcgt
     1021 aattagcctc tatctcagag acgcaggact gtatctcctc ctctgaaagc gggggcactt
     1081 cgacgtggaa cacggcttct cgctcggcgg gtgtggcgtc catggcgtcg agcaacctgt
     1141 gaattgtctc aggcttgggc cgcgagcggc tagaccgacc tcgttccatg cggttgagaa
     1201 aggcggcatc cacgttcgcc aaccgggcca gcgtcttttg ttgcagccca cgccgcactc
     1261 tcagttgctg gatgcgccag cccaggttgg ttgtattagg gcggtcgagc gggttctggt
     1321 cggacaacgg ggacctcatc acagagcata gtcatcatag agcctggcaa gtggtgggac
     1381 gctctacacg ggctgacggc cagttcagat gtggagagca gcaaaccgcg aagaaatcgg
     1441 cgcacacaaa gccagcccta taccaccgag gtcacggagc agcacacggt ggtcgcgctt
     1501 acgtatcgcg gtccgctccc ggcgcgggca gcgcgcgggg gcgaacaccc gcaacacaac
     1561 ccggcccccc ggtaaagatc ggaggcgtca ccacggccca atccggtaaa gtccccgcgg
     1621 tcgtgggccg gcccggatcg tgtttggttg cggaacaagc gccctatcgg accattggcg
     1681 cggtcaattg ccaccatagt cctgtaggag ttggcgatag tatataccat tagttaagct
     1741 ttaatcaata gggggatgca cttacgctct gcaaatatag cagattttga cttagcgccg
     1801 gtgcagactg acacgcttgg gccgttcggg ggtgttcaag cgcgcgtttg gccgcttcag
     1861 gccccgctgc cgggcagctt ttcgcgctgg attacggacc agacgtggtc ggccagccag
     1921 cggtagcccg cgctgcttgg gtgaaacctg tccgccgaca caaagtcggg acggataagc
     1981 acctgctcgc tgccggggaa gaggtctacc aacgtcacgc ggtcgccgta gtccgcggcg
     2041 gcctgcgaca tggcggcgtt ccactggcga ataccgccct gtatcaactc tcggcgggcg
     2101 gaatcagacg cgggcaccag caaagagaga tcgggcaggt tgagcaacag gacgtgggcc
     2161 tcggtgtcca gggtaaggcg gtcgagggcg gagcgcaggt cgcgggtgta aaggggcaga
     2221 gggatgcccc gcgtggcatc gttcacgcag ttccacagcg tgacgaggtc gggctgcgcc
     2281 gccaccgcct gcggcacctc gaccttgttt gcatcgttga gggtaatgcc gttgcggccc
     2341 agcttcacga gccgcgtgtt cgcaggcaga tagcgcgcca gcaattctac ccagctttcc
     2401 gaggccgggt cggttgcccc cactcccacc acgtccgagg caccgattcc cgcatacaca
     2461 aaaggttgtg gctcctgcac ctgctcctgc tcctgctctc cttcgtcctg gacgctgggg
     2521 tcgggaggca cggacactgg cgtgcgtacc gcccacctcc agacaaagaa cgtgagcacg
     2581 atgatggcaa gaacaaggcc aatcacgacg ggcgatatgg gagaaacgag caaggaatct
     2641 acctttcggg gcttgcgaac cgaggttcag tcggctagtg cagcacctgc cgcgataagg
     2701 cccatgccca caaccgccga caggattagc cacgtacccc ccactgtgac ctgcgtgctg
     2761 ccggcgaccg cgttgaacac aagatagaac ggcacgagca gcagcaagat cgcccccacg
     2821 cccaccacca cccacgatat ggcccggcga gaggaacgcc tgccaacgtc ttgagacaaa
     2881 ttaggcctcc ctgagtgtat aatt
//
LOCUS       DASRWZ010000766         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_130624, whole genome shotgun sequence.
ACCESSION   DASRWZ010000766 DASRWZ010000000
VERSION     DASRWZ010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130624"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..2747
                     /gene="lon"
                     /locus_tag="VFH60_12170"
     CDS             213..2747
                     /gene="lon"
                     /locus_tag="VFH60_12170"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6314581.1"
                     /translation="MAGNGERTPDSINEQAAQPEVTSAGIADTPIVVPLLPLVENVVF
                     PKAVYPLVLQGEQAESLLGAAESSNRLVALFLQREGAGAEPSASDLYGVGTLSRLSEI
                     KRGQDGVIEVTAEGIAPVQLLNIKQWNPHVTAEVEAIEPLNEQDPALESLAARTLELY
                     RGLVSADPTAAARLQPVIEETRDPLDVAYLVAATIPISPAARQAILEARSPSAMLLQL
                     LTFLNKAQEMSANSHANGEATRNVVSLPARSASLDQAVLDRTDLEMQLRDAGMPPTAW
                     RVAQRELDRFSLLTPTSPDYNLVRTYLQWLADLPWQNGYEPDIDLDRARAILDRDHYG
                     LEEVKLRIIEHLAVRKLRRQRASDNQAGCDVEAHPDALAQGQPPTQPVLCLVGPPGVG
                     KTSLGRSISEALNRPFVRISLGGVSDEAEIRGHRRTYLGAMPGRIVQSLARVESSQPV
                     MMLDELDKMGGNGKGDPTAALLDVLDPEQQTEFVDHYIEVPFDVSCVFFLATANTLES
                     VPEALRDRLEVIRLSGYTEEEKAQIAARHLVPRQMLWHALNDGDMRWEPDAVLQIIRS
                     YTREAGVRQLEREIATVCRRVATLVAQDSDVTSRFPFVIDTAFVTDVLGTPRFLPAEP
                     EATDQPGVVTGVFWTPVGGDIMHIEALMMPGSKTLTITGQLGEVMRESAQTALSYVRA
                     RAEDLRIAPDFYERHDLHLHIPSGAVAKDGPSAGIALAVAIVSLLTDTPVSGDVAMTG
                     EITLRGRVLPVGGIKEKVLAARRAGVHKVVLPAQNRRDLDDVQPEILQDLELVFVDTM
                     DEVLAAAFPLSAKGLPASNKTTRKEQANPRATMLDVPSAARAMHQQ"
     gene            2882..>3211
                     /locus_tag="VFH60_12175"
     CDS             2882..>3211
                     /locus_tag="VFH60_12175"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HET6314582.1"
                     /translation="MATVVGLFDNRNQAQMAVEQLRAMNIAAGDISVAMQSSTAGTTE
                     VVTETGAGAGAGAVTGAVGGGVLGGLAGLLVGVGALAIPGIGPVVAAGPLATTLIGAG
                     VGAATGGR"
BASE COUNT          645 a         1041 c          989 g          536 t
ORIGIN      
        1 cgcctgcctg gtgggggcgg ttcgcgcacc gcccctactg ttcttttgtc aggtgttgta
       61 aacaaaataa gtggcatggt tgttgcacta gagcatatca cattatctac tccagtacgt
      121 ccccccaagc ggcactctta cacgcttcta atatccccac gcggcaacgc gcctgggcag
      181 catggcatca gcctgctaga acggatgaag atatggcagg aaacggagaa cgtacacctg
      241 atagcataaa cgaacaagcg gcacagcccg aagtaacaag cgccgggatt gccgatacgc
      301 ctattgtagt ccctttactc ccgctcgttg agaacgtcgt cttcccgaag gccgtatacc
      361 cgcttgtctt gcagggcgag caagccgaaa gcctgcttgg ggcggcggaa agctcgaaca
      421 ggctggtcgc gctattcttg cagcgagaag gcgcgggtgc cgagccttca gcgagcgacc
      481 tgtatggtgt gggcaccctc tcgcgcctga gcgagataaa gcgtgggcaa gacggggtga
      541 tagaagtcac agccgagggt atagcgcccg tccagttgct caacataaag cagtggaacc
      601 cgcacgtcac ggctgaagta gaggccattg agcctcttaa cgagcaagac cccgcgcttg
      661 agagcctggc ggcgcgcacg cttgagctat atcgagggct ggtctccgcc gaccccacag
      721 ccgccgcccg cttgcagccc gtcatagaag agacacgcga ccccctggac gtggcttacc
      781 tcgtggcggc taccatcccc atcagcccgg cagcccggca ggccatcctt gaagcccgca
      841 gcccctcggc catgctgctg caactcctta ccttcctgaa taaggcacag gagatgagcg
      901 cgaactctca cgccaacggg gaggcaaccc gcaacgtcgt cagcctgccc gcgcgctctg
      961 cctcgctcga ccaggcggtg ctcgaccgga ctgacctgga aatgcaactg cgcgacgcgg
     1021 gcatgcctcc taccgcgtgg cgcgtggccc agcgcgagct tgaccgcttc agcctgctca
     1081 ccccgacctc cccggactac aacctcgtgc gtacctacct gcaatggctg gccgacctcc
     1141 cctggcagaa cggctacgaa cccgacatag acctggaccg cgcccgcgcc atcctcgacc
     1201 gcgaccacta cgggctggaa gaggtcaagc tgcgtatcat agagcacctg gcggtacgca
     1261 agctgcggcg gcaacgggcg agcgacaatc aggcgggctg cgacgtagag gcccaccctg
     1321 acgccctggc ccaggggcag cctcccactc agcccgtcct gtgcctggtc gggcctccgg
     1381 gcgtcggcaa gacctccctg ggacgctcca tctcggaggc gctgaaccgt cccttcgtgc
     1441 gtatctccct gggcggggtg agcgacgagg ccgagatacg cgggcaccgc cgtacctacc
     1501 tgggcgctat gcccggcagg atcgtgcagt cgctcgcacg cgtggagagc agccagcccg
     1561 tgatgatgct ggacgagctg gacaagatgg gaggcaacgg caagggagac cccacagccg
     1621 ccctcctcga cgtgctcgac cctgagcagc agaccgagtt cgtggaccac tatatagagg
     1681 tgccattcga cgtgtcgtgc gtcttcttcc tcgccaccgc caacactctc gaatcggtac
     1741 ccgaagccct gcgtgaccga ctggaagtga tacgtctctc aggctacacg gaggaggaga
     1801 aagcgcagat agcggcccgc cacctcgtcc ccaggcagat gctctggcac gcgctcaacg
     1861 acggtgatat gcgctgggag ccggacgccg tgctgcaaat catacgcagc tatacgcggg
     1921 aggcgggcgt gcgccagctt gagcgcgaga tagccactgt ctgccgccgc gtagccaccc
     1981 tcgtcgccca ggacagtgat gttaccagcc ggttcccatt cgtgattgat accgccttcg
     2041 ttaccgacgt tctgggcacg ccgcgcttcc tgcctgccga gcctgaggcc accgaccagc
     2101 cgggcgtcgt tacgggcgtt ttctggacgc ccgtgggtgg cgacatcatg cacatcgaag
     2161 cgttgatgat gcccggcagc aagaccctca ctatcacggg ccagttgggg gaggtgatgc
     2221 gagaatctgc acagacggcg ctctcgtacg tgagggcgcg agcggaggac ttgcggatag
     2281 ctcccgactt ttacgagcgc cacgacctgc acctgcatat accgtcgggc gcggtagcga
     2341 aggacggccc ctcggcaggc atcgcccttg ccgtagcgat agtatctcta ctcaccgaca
     2401 cccccgtttc aggcgacgta gctatgaccg gcgaaatcac cctcaggggc agagtgctac
     2461 ccgtgggcgg gataaaggag aaggtgctgg cggcgcgtcg cgcgggcgtt cacaaggtcg
     2521 tgctgcccgc ccagaaccgg cgcgacctgg acgacgtgca acccgaaatc ctgcaagact
     2581 tggagcttgt attcgtggat acgatggacg aggtgctggc ggcggctttc cctctctcag
     2641 cgaagggctt gcccgcgtcc aacaagacca cgcgtaaaga gcaagccaac ccgcgtgcta
     2701 ccatgctcga cgtgccaagc gcggccaggg ctatgcacca gcaatgatta tgtctggtgg
     2761 cacactcatt gcttatacca tgcttgccgc agtaaacgcg tctcatatgg cggcacgggc
     2821 gaccagagac gcgagcggac ataaccggaa cactctaaaa taagtaggag gtactcccct
     2881 aatggcaaca gtagtaggtt tattcgacaa tcgcaaccag gcccagatgg ccgttgagca
     2941 acttcgtgct atgaacatcg ccgcaggcga tataagcgtg gcaatgcagt ccagcaccgc
     3001 agggaccaca gaagtagtaa cagagaccgg cgcaggcgca ggtgcaggcg cggtaacagg
     3061 cgcagtaggc ggtggcgtgc tgggcggcct ggcaggcttg ctcgtgggcg tcggcgcact
     3121 cgccatcccc ggtataggcc ccgtagtggc tgccggaccc ctcgccacca cattgatagg
     3181 cgcgggcgtc ggtgcggcga ccggcggccg c
//
LOCUS       DASRWZ010000767         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_267114, whole genome shotgun sequence.
ACCESSION   DASRWZ010000767 DASRWZ010000000
VERSION     DASRWZ010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267114"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..645
                     /locus_tag="VFH60_12180"
     CDS             382..645
                     /locus_tag="VFH60_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314583.1"
                     /translation="MEDTVAPGTRPAPPHTHQAGPASPEPATLTLQDLLDLPRQLLPE
                     ETYRHLQNATRETLLAGYTLWQHLSKSMSANSGKRVRKRIDVE"
     gene            741..>2255
                     /locus_tag="VFH60_12185"
     CDS             741..>2255
                     /locus_tag="VFH60_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6314584.1"
                     /translation="MADATHHDDNPIKGQGKRKRPAAEGSENGSPQQARTRRKAGAAN
                     LPPFIIVDDVAYLDPEEENLAASKEKIDISSMDDLDMAERFDLGDTISRSGGARGDSL
                     VDLLDEAFDTLALDEADGDSRPGKVERTITRTSASISSVDANGIVGRVEIDEIAITEP
                     TAETEFEEEGEPDLDELVKVDAAVEVDKPEELERFIDRALTMEGVSVDDSVRIYLREI
                     GRTALLKSPDESALAERMTQGRNAVVEMEMLKETLNDGSLDPAVIEARMRELEALKRS
                     GDQAKQILVQANLRLVVSIARRYIGRGLSLLDLVQEGNIGLMKAADKFDHRKGFKFST
                     YATWWIRQAITRAISDQSRTIRLPVHVTEMVTKLRRAAHELQQQLLREPTEEELGKEL
                     GMPAERVRRILDMARHPASLDAPMEEGEDSFLGDFIEDESTGAPEEEASRQMLKEQVE
                     DILSKLTERERKILEMRYGLADDRPRTLEEVAREFNITRERIRQIETRILRKLRY"
BASE COUNT          495 a          740 c          686 g          334 t
ORIGIN      
        1 ttcgccacca aggagcaggt ggacctgctg caaacgggtg acgagcttgt ggtgcaggtg
       61 ggcgactaca agcgcaacct gatcctgccc cgcgccctgg ctactttgca ggtggcaggg
      121 gccaagctgg acaacggcga gctaaggatc aacttcgcca aggcctagcg tatagccgcc
      181 aggcgcttgc cgggactacc ggtcacttat ttgacggcat gcaatagtcc tacccgcttt
      241 gaccggcagc ggttttcctt gcaatgtaac attgacggtg gtatagttgg gtacctggag
      301 gacgggtgac tgaaagcccc cgtgcggcac gcgtgagaga gggtaccagt cgaagccccc
      361 gccccagcga gagggagaca aatggaggat acggtagcgc cagggacccg gcccgcgccg
      421 ccccatacgc accaggcagg gcctgcctcg ccggagcctg ccacccttac cttgcaagac
      481 ctcctcgatc tgcccaggca gcttctaccc gaggaaactt accggcacct ccaaaacgcc
      541 acccgtgaga ccctgctggc cggctacacc ctgtggcagc acctcagtaa gtccatgtcc
      601 gccaactccg gcaagagggt ccgcaaacgc atcgacgttg agtaattact aatcacgaat
      661 tactgaggaa caaacgtaac tcgtagctca caacccgtaa ttcgtaatta gtaattcgta
      721 attcggagaa tccgctacag atggccgacg ccacccacca cgacgacaac cccataaagg
      781 gccagggaaa gcgcaaacgc ccggctgccg agggtagcga gaacggctct ccccagcagg
      841 cgcgcaccag gcgcaaggcc ggggcggcca acctgccccc cttcatcatc gttgacgatg
      901 tagcctacct cgaccccgag gaggagaacc tcgccgcgtc caaggagaaa atcgacatct
      961 cctctatgga cgacctcgac atggccgagc gcttcgacct gggtgatacg atctcccggt
     1021 cgggcggggc gcgcggcgac agcctcgtgg atttgctgga cgaggcgttt gacacgctgg
     1081 ccctcgatga ggcggacgga gatagtcgcc ccggcaaggt agagcgcacc ataacacgca
     1141 ccagcgcctc catcagcagc gtagacgcca acggcatagt gggccgcgtg gagattgacg
     1201 agatagccat tacggagcct accgccgaga ccgagttcga ggaagaaggc gagccggacc
     1261 ttgacgagct tgtgaaggta gacgccgccg ttgaagtgga caagccggag gagcttgagc
     1321 ggttcatcga ccgcgccctc actatggaag gcgtgtcggt ggacgactcg gtgcgcatct
     1381 acctgcgcga gataggccgc accgccctcc tcaagtcgcc cgacgaaagc gccctcgctg
     1441 agcgcatgac ccagggccgc aacgccgtcg ttgagatgga gatgctcaag gagaccctca
     1501 acgacggcag cctcgacccc gccgtgatag aggcccgcat gcgcgagctt gaggccctca
     1561 aacgttcggg cgaccaggcc aagcagatac tcgtgcaggc caacctgcgc ctggtggtga
     1621 gcatcgcccg ccgctacata ggccgcggcc tctcgctgct cgacctcgtg caggaaggca
     1681 acatcggcct gatgaaggcg gcggacaagt tcgaccaccg caagggcttc aagttctcca
     1741 cctacgccac ctggtggata cgccaggcca tcacccgcgc catctccgac cagagccgca
     1801 cgattcgcct ccccgtgcac gtcaccgaaa tggtcaccaa gctgcgccgg gccgcccatg
     1861 agcttcagca gcagctcctg cgcgagccga ccgaggagga gcttggcaag gagctgggta
     1921 tgcccgccga aagggtgcgc cgcatactcg atatggcccg ccaccccgcc tcgctagacg
     1981 cgccgatgga agaaggggaa gacagcttcc tgggtgactt catcgaggac gagagcacgg
     2041 gcgcgccgga ggaagaggca agccgccaga tgctcaagga gcaggtcgag gacatcctga
     2101 gcaaactgac cgagcgcgag cgcaagatcc ttgagatgcg ctacggcctc gctgacgacc
     2161 gcccccgcac gctcgaggaa gtggcccgcg agttcaacat cacccgcgag cgcatccgcc
     2221 agatagagac ccgcatcctc cgcaagctgc gctac
//
LOCUS       DASRWZ010000768         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1363265, whole genome shotgun sequence.
ACCESSION   DASRWZ010000768 DASRWZ010000000
VERSION     DASRWZ010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363265"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1947
                     /locus_tag="VFH60_12190"
     CDS             160..1947
                     /locus_tag="VFH60_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6314585.1"
                     /translation="MNDGNPNPNLRTDSPDRWGEPGAPVPSDILEQLDRDIAASGGEW
                     REPVEYVGAVGRTMFDLPTSEDNTVTVLLPREEIGKVPSQSIVRVRSVGDGRVYLGIV
                     VKGPFAEPDGLRGDAPIVITTAVRGGIFMPRYHGRVQVELIGEQVGGVVVPPRLRPLP
                     NSPVFILDTDETMDILRLGGDITLGVPVGYEQLTASVPSNKKSVLPRHLGILGTTGGG
                     KSTTVSGLIASFRGAGVATVVLDTEGEYTQIYQPTTDPTMLTTLEKRGLEPRGVKGVR
                     VYHLQGRTTTCPDPRRVTAFSLQFAQLSPYLVMEVLDLNEAQSQRYGKAYDITKRLLR
                     QLNIFPSNAEERDERLELDEMETGYPRMTVAHMYDVVKACVEAVSGEEGDGKFYSPDF
                     KQAGKEIAKAIKAADIPSHIGSWRVVQGRLSQLLRLHIFDNPSAGPLDYAALTRPGTV
                     SIIDLSDTDSPQINNLVIAELLRGLQGAQNDNYRRSEQGGEQRRLMIIVEEAHEFLSD
                     ERIKQMPVLFQQVARIARRGRKRWLGLCFVTQLPQHLPDEVLGLINNYILHKISDANV
                     ISRLRRSLGVIDESLWSRLPNLAPGQAIV"
BASE COUNT          430 a          679 c          729 g          373 t
ORIGIN      
        1 gggagttcag gggtaagtac gttgcggttg gtgcggcccc ggtgttttcg ggggctgatg
       61 gcgaggatgt gcagtcggcg ctggtgccgt tcccggacgg gagcgtgcag agggcggagg
      121 cttattgaag tcgaaggtcc acgcaaggag gtaggcccgt tgaacgacgg caaccccaac
      181 cccaacctac gaaccgactc tcccgaccgc tggggagagc cgggcgcgcc cgtgccctcc
      241 gacatcctgg agcagcttga ccgggacatc gccgcctccg ggggcgagtg gcgggagcct
      301 gtggagtacg tcggggcggt ggggcgcacg atgttcgacc tgccgacgag cgaggacaac
      361 acggtcaccg tgctcctgcc gagggaggag ataggcaagg tgccttcgca gtccatcgtg
      421 agggtgcgca gcgtgggtga cgggcgagtg tacctgggca tcgtggtcaa ggggccgttt
      481 gcggagcctg acgggctgcg gggggatgct cctatcgtga ttaccacggc ggtgcggggc
      541 ggcatcttca tgcccaggta ccacgggcgg gtgcaggtgg agcttatcgg cgagcaggtg
      601 ggcggggtgg tggtgccgcc gagattgagg ccgctgccca acagccccgt cttcatcctc
      661 gatacggacg agacgatgga catactgcga cttggtggcg atataacgct gggcgtgccg
      721 gtggggtacg agcagcttac cgcctcggtg ccctccaaca agaaatcggt gctgccccgg
      781 cacctgggca tcctgggcac cacgggcggc gggaagtcta ccaccgtatc gggccttatc
      841 gctagcttcc gcggggcggg cgtcgccacg gtggtgctcg acaccgaggg ggagtacacg
      901 cagatttacc agcccaccac cgaccccacc atgctcacga cgctggagaa gcggggcctt
      961 gagccacggg gggtgaaggg ggtgagggtg taccatctgc aaggccggac caccacctgc
     1021 cccgacccca ggcgcgttac ggccttttcg ttgcagttcg cccagctttc gccctacctg
     1081 gttatggagg tcctcgacct gaacgaggcg cagagccaac ggtacggcaa ggcttacgat
     1141 ataaccaagc gcctcctccg ccagctaaac atctttccct ccaacgccga ggagcgagac
     1201 gagcgacttg agcttgacga gatggagacc ggctacccgc gcatgacggt ggcccacatg
     1261 tacgacgttg tgaaggcgtg cgtggaggcg gtgtcgggcg aggagggtga cggcaagttc
     1321 tacagccccg acttcaagca ggccggtaaa gagatagcaa aggccatcaa agccgccgac
     1381 atcccgtcac acatcggaag ctggcgggtg gtacagggcc gcctgagcca gctactgcgc
     1441 ctgcacatct tcgacaaccc cagcgccggg ccgctcgact acgccgccct tacccgcccc
     1501 ggcacggtct ccatcatcga cctgagcgac accgactcgc cccagatcaa caacctggtg
     1561 atagccgaac tgttgcgggg tctgcaaggt gcccagaacg acaattacag gcggtcggag
     1621 cagggcgggg agcagcgccg gttgatgata atagtggagg aggcgcacga gttcctgtcg
     1681 gacgagcgca tcaagcagat gccggtgctc ttccagcagg tagcccgcat cgcacgccgg
     1741 ggccgcaagc gctggctcgg gctgtgcttc gtgacgcagc ttccccagca cctgcccgac
     1801 gaggtgctcg gcctcatcaa caactacatc ctgcacaaga tcagcgacgc caacgtgata
     1861 agccgcctca gacgctcgct cggcgtaatc gacgaatcgc tctggagccg ccttcccaac
     1921 ctcgcccccg gccaggcgat cgtctagatg agcagcttcg cccgccccct gctgctcaac
     1981 atcgacccca cgccctgcaa gctgcgcatg gttgactagg ggaacagaag agacgaaaac
     2041 gcaaagacgc aaagaacgca aagaacgcgc agagattagg attgtggtgt ggtacacaaa
     2101 gaggaacagg gtacgtggtc atgtaccctg ttcctctttg tgtactctct aaaccctccc
     2161 taatctttgc gcgttctttg cgtctttgcg ttttcgtctt aaccaacgct a
//
LOCUS       DASRWZ010000769         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1158360, whole genome shotgun sequence.
ACCESSION   DASRWZ010000769 DASRWZ010000000
VERSION     DASRWZ010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158360"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1968
                     /gene="aspS"
                     /locus_tag="VFH60_12195"
     CDS             142..1968
                     /gene="aspS"
                     /locus_tag="VFH60_12195"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET6314586.1"
                     /translation="MQVELGQSGQAGYERVSCGQLRSQDDGRQVQLNGWVHRRRDQGA
                     LIFIDLRDRDGITQVVLNREQNSQAHKLAESVRSEYVLRVEGTVQLRGAERVNPNLGT
                     GEVEVVAGQVTILNEAKPLPFEIASGADADEMLRLKYRYLDLRRPRMQRNLILRHRVV
                     KFIRDYLDERGFVEIETPMLTNETPEGARSYLVPSRIHPGEFYALPQSPQQLKQLLMV
                     SGMMRYFQIARCLRDEDLRADRQPEFTQLDLEMSFVQRDDVLDLMEGLFAELTERVSE
                     KKVLFKPFPRLTFEESMRRFGNDKPDMRFGMELTSLEDVLRAGSFAVFTSAIESGGSV
                     AGIRVPGAGSYTRRELDELTETAKRYGAKGLVWFAVEEKAEGGVQFRGPAAKFLTEDE
                     TGGIVAAFGAEVGDLLLIVAGDTRSTQDVLGRLRLDMGRKLDLIDESVMAYAWIIDPP
                     LFEWNAEEERWDSTHHPFTSPLPEDAHLLETDPGQVRAAAYDIVCNGYELASGSIRIH
                     ERGLQFRIFELMGYAAEDIEVRFGHLLAAFEYGAPPHGGIAPGIDRLVMLLASEANIR
                     EVIAFPKTAQARDLMMGAPSPVSARALKELHIRVVGEQPQGN"
BASE COUNT          428 a          660 c          678 g          357 t
ORIGIN      
        1 aagggctgtt tgggtgctga agggcgagat tcttagctgc gctcagaatg acaaaggcga
       61 agtttggcta tcgtcagttc aacactttta ttcttgagat agcttctaac aagaatatca
      121 cgggacacaa aggagacagg catgcaggtc gaacttggac agagcgggca agcggggtat
      181 gaaagggtaa gctgcggcca gcttcggtcg caggacgacg ggcggcaggt gcaactcaac
      241 ggctgggtgc acaggcggcg cgaccaagga gcgctcatct tcatcgacct gcgcgaccgc
      301 gacggcatca cccaggtggt gctcaaccgg gagcagaaca gccaggccca caagcttgcg
      361 gagagcgtgc gctcggagta cgtgctccgg gtagaaggca cggtgcagct tcgcggggcc
      421 gagcgggtga accccaacct cggcaccggc gaggtggaag tggtagcggg gcaagttacc
      481 atcttgaacg aagccaagcc tctgcccttc gagatcgcga gcggggcgga cgcggacgaa
      541 atgctgcgcc tcaagtatcg ctacctcgac ctgcgccgtc cccgcatgca gcgcaacctg
      601 atattgcggc accgggtcgt caagttcatc cgcgactacc tggacgagcg cggtttcgtg
      661 gaaatcgaga cccccatgct caccaacgag accccggagg gcgcgcgctc ctatctcgtg
      721 cccagccgca tacaccccgg cgagttctac gccctgcccc aatcgccgca acagttgaag
      781 cagcttctca tggtgtccgg catgatgcgc tacttccaga tagcccgctg cctccgcgac
      841 gaggacctgc gcgccgaccg ccagcctgaa ttcacccaac tcgacctcga aatgagcttc
      901 gtgcaacggg acgacgtgct cgacctgatg gaagggctgt tcgcggaact caccgagcgg
      961 gtgagcgaga agaaggtgct gttcaagccg ttcccccgcc tcaccttcga ggagtcgatg
     1021 cgccgctttg gcaacgacaa gcccgacatg cgctttggca tggagcttac ctccctggaa
     1081 gacgtgctgc gtgccggcag cttcgcggtc ttcacgtcgg ccatagagtc ggggggcagc
     1141 gtcgcgggca ttcgcgtgcc gggcgcgggc agctacaccc gccgcgaact tgacgagctt
     1201 accgaaacgg ccaagcggta cggcgcgaaa gggctggtct ggttcgcggt agaggagaaa
     1261 gcggaagggg gcgtgcagtt ccgtggcccc gccgctaaat tcctcaccga ggacgagacc
     1321 ggcggcatag tggcggcttt cggcgcggag gtgggcgacc tgctgctaat cgtagctggc
     1381 gacacccgct ccacccagga cgtgctagga cgcctccgcc tcgacatggg ccgcaagctg
     1441 gacctcatcg acgagtccgt gatggcctac gcctggatta tcgacccacc cctgttcgag
     1501 tggaacgcgg aggaggagcg ctgggactcc acgcaccacc ccttcacctc gccgctgcct
     1561 gaggacgcgc acctgcttga gaccgacccc ggccaggtgc gcgccgctgc ctacgacatc
     1621 gtgtgcaacg gctacgagct tgccagcggc tccattcgta tccacgagcg gggcctccag
     1681 ttccgcatct tcgagctgat gggctacgct gccgaggaca tagaggtacg cttcggccac
     1741 ctgctggcgg cgttcgagta cggcgctcca ccgcacggtg gcatcgcccc cggcatcgat
     1801 cgcctggtga tgctcctggc aagcgaggcc aacatacgcg aggtgatagc cttccccaag
     1861 accgcccagg ctcgcgacct catgatgggc gcaccctccc ccgtctccgc acgcgccctc
     1921 aaggagctac acatacgggt tgtgggcgaa cagccacagg ggaactaaac gccaagacgc
     1981 caaggaccac gccaaggtat taaggtttgg agagttgagc gagttaggtc agcaggcagt
     2041 ataggggcgg tgctcacgat aagagtaaag ttcccatggg tattccttaa gtgggaactt
     2101 tacttttgga gacggaccag ggt
//
LOCUS       DASRWZ010000770         4926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_541961, whole genome shotgun sequence.
ACCESSION   DASRWZ010000770 DASRWZ010000000
VERSION     DASRWZ010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541961"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="VFH60_12200"
     CDS             complement(<1..1411)
                     /locus_tag="VFH60_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET6314587.1"
                     /translation="MDTSTEGSGAGPRKNRQLLVGAGIAFALLLLVGVGVWRALSQTP
                     PGENGPRAGATRTPGPIANTQSGLNGWVSVYTPGPTRTPDARLGTPGTATPQPTETTG
                     TSDGQVESTPTIVAGAATFTPEAGQGPAGTDDKPRVLGITAGVGMRSGDIAGAAPLAV
                     TFSEAMDQASAQSAFLLRPQVPGKFAWTANTLIFTPDQSLQPSTSYTVTLASDARSQS
                     GESVAAPLSASFQTAPPPAVLRTLPSAGASEVPTDTIVTITFNRPMIPLTALDSQPDP
                     SQWVTISPNVAGRWVWLGTAAVGFHADTGFLPSTAYRVDVKAGWPDAAGVTLQQGASV
                     SFTTVRPAILNVSPGNGSARVPIDAPIVVSFNIPMDHASVESNISLPLPSAAEWSSDS
                     TVVTFTALSLLEFSRSYTVQVTGSLKPARGNAAELTGGASANRWSFTTTGATHVENHY
                     PDSTGGPASPGESFGFSFNN"
     gene            2126..4585
                     /locus_tag="VFH60_12205"
     CDS             2126..4585
                     /locus_tag="VFH60_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HET6314588.1"
                     /translation="MSDIVTNRAYGGNGNGNGHENGHENGHGNGSNGHGGRNGSAGSN
                     GHGNGHAGDPVKPAQGSRLLIVSNRLPFTAQEEKGDLTLKPSSGGLVSGLTDYLESLR
                     DPGIDEVTPENAPYLWVGWPGATVARESEEKLKAIALDRFHAYPVFVDEEAMEKFYHG
                     FCNKTIWPLFHYLPSFAEFREEYWEQYKAVNQVFADAVLEIARPGDTIWVHDYQLMLL
                     PRLIRDRMPGVTVGFFLHIPFPGYELFRTLPRKWATAILEGMLGADLVGFHTHEYTQY
                     FSRTVLRELGYEQNLGEILVSDRLVKVETFPMGIDFNRYHDAVSTPEVERERSLLQRS
                     VQDTKLVLSIDRLDYTKGIINRLEGFARFLEEYPEWHGRVMMALVIVPSRIGVERYQA
                     TKERIEEIVGRTNGRFGSITWTPITYQFKSIPFPLLVAMYNVSDVALITPLRDGMNLI
                     AKEYVAAHSGNGGVLILSEFAGAAKELGDAIIINPNSREEIAQAIREALEMPQDEQDR
                     RMERMQDILRRFDITYWAEDFMGQLQATREAQEKLKARLMSDTARERMLGDYRYASKR
                     LLLIDYDGTLVPFNKDPQQARPGDDVVQLLARLCDTPGSQVVLISGRDKETLDRWFGD
                     LPVAMVAEHGVWIKQPHESEWTMSQPLTSEWKSRIRPVLEMYANRLPGSFLEEKDYSI
                     AWHYRGADPDLGPSRAKELLDHLVNFTANINVQVLQGNKVIEVKTSGVSKGTAGRTWL
                     DGGDFDFVLAIGDDWTDEHLFMVLPESAYSLRVGVVQSYARYNLRSYTEVTRLLEELS
                     HNTPAEPALYGEQQAQPVHAS"
BASE COUNT          980 a         1493 c         1610 g          843 t
ORIGIN      
        1 ggttgttgaa cgagaagccg aacgactcgc ctggagaggc gggaccgccc gtgctatcgg
       61 gataatggtt ctccacgtgc gtggcaccgg tcgtggtgaa gctccagcgg ttggccgaag
      121 ccccgcccgt aagctcggcg gcgttgcccc gcgcgggctt gaggctgccg gtgacttgca
      181 cggtgtagct gcggctgaat tccaggagac tgagcgccgt gaacgttacc acggtgctgt
      241 cggacgacca ttcggccgcg ctcggtagcg gcaggctgat gttgctctcc accgaggcat
      301 ggtccattgg aatgttgaag ctcaccacga tgggcgcatc tatgggcacg cgcgccgacc
      361 cattgccggg cgacacgttg aggatggcgg ggcgcaccgt ggtaaagctc accgaagcgc
      421 cctgctgcaa cgtcacaccg gcggcatcgg gccacccggc tttcacgtcc accctgtagg
      481 cggtcgacgg gaggaagccg gtatcggcgt ggaagcctac cgccgccgtg ccgagccaca
      541 cccagcggcc cgctacgttg ggcgaaatcg tcacccactg cgaggggtcg ggctggctgt
      601 ctagcgcggt gagggggatc atcgggcggt tgaaagtgat agtgacaatc gtgtcggtgg
      661 gcacctcgct ggcaccggcg ctcggcagcg tgcgcagcac ggcgggaggg ggtgccgtct
      721 ggaagctggc agacagggga gcggccacgc tctcgccgct ctgcgaccgc gcgtcggagg
      781 ccagggtcac ggtgtaggag gtgctgggct ggagcgactg gtcgggcgtg aagatgaggg
      841 tgttggcggt ccaggcgaac ttgccgggca cctgcggcct caagaggaag gccgattgcg
      901 ccgaagcctg gtccatagcc tcggagaaag tcactgcgag gggcgccgcc cccgctatgt
      961 cgccggagcg catgcccacg cccgccgtga tgcccagcac gcggggcttg tcatcggtcc
     1021 cggccggccc ctgccccgct tcgggcgtga aagtggcagc gccagcgacg atggtgggcg
     1081 tgctctccac ctgcccatcg gaggtgccgg tcgtttcagt aggttgtggt gtcgcggtgc
     1141 ctggggtgcc cagacgtgcg tccggcgtgc gggtcggccc tggcgtgtag actgagaccc
     1201 agccattcag tcccgactgg gtgttggcaa tagggccggg cgtgcgagtg gcccccgccc
     1261 ttggcccgtt ttcgccgggc ggagtttggg agagtgcccg ccacacaccc acacccacga
     1321 ggagcagcaa ggcaaacgcg atgcccgccc ccactaatag ctggcgattc ttgcgaggtc
     1381 cggccccgct accttccgtc gaggtatcca ctacgacaac ccccctggtg aattttggat
     1441 ttgggatttc ggattgcgaa tttagggacg catctccgat atcaattaca tgacgatggt
     1501 ggtatgcata aagttcccgc acctgatagg aggtggggga ttgactggca tatgcagtat
     1561 atcattttgg agagggagtt gtgaatcttg ggctttcagg atagtcgctg ggctgtagtg
     1621 aagtgatgaa ttgtgaaggc cgcacggcgg tcgagaccac actcccactg tcatcctgag
     1681 cgtagcgaag gatcaggtag gcaggcgaga gtctacaacg acggggatga tgtgggaagg
     1741 aggaacactg gcgccgcaat tggaacggtc aagagcagga ggctgatcct tcgctgcgct
     1801 caggatgaca ggggggagtg tggtcacaca atcgtagagg gcgctttgca cgacttggac
     1861 tgggatcgct tacgttaaca aacgtaaaat cgtccggccg gcagcctgcg tgccccggcg
     1921 gtggtacgca acggtggcac atgccttgca cggtccaggt agtaggacca aactaaggta
     1981 tgtagttgtg agaagtagaa gaagcaagag ttgaggaagc tgagtgtgag cagggcgcgc
     2041 aaccaaaggg cgggacacgc cgccaatagc ctatatccgt gcgtccggca accacgctcg
     2101 tcctgagaaa ggactcgcgg tctgcatgtc tgacatagtc acgaatagag cttatggcgg
     2161 caacggcaac ggcaatggcc acgagaatgg ccacgagaac ggccacggca acggcagcaa
     2221 cggccacggc ggcaggaacg gcagcgcagg gagcaacggc cacggtaatg ggcatgcggg
     2281 cgacccggtg aagcccgcgc aaggctctcg cctgctcatc gtatcgaacc gcctgccctt
     2341 cacggcgcag gaagaaaagg gcgacctgac cctgaaacca agctccggcg gcctcgtttc
     2401 cggcctcacc gattacctgg agagcctgcg cgaccccggc attgacgaag ttacccccga
     2461 aaacgcccct tatttgtggg tgggctggcc gggggccacc gtcgccaggg agtcggagga
     2521 gaagctcaag gcgatagccc tcgatcgctt ccacgcctac cccgtcttcg tggacgagga
     2581 ggcgatggag aagttctacc acggcttctg caacaagact atctggcccc tcttccatta
     2641 cctgccctcc ttcgccgagt tccgcgagga gtactgggag caatacaaag cggtaaacca
     2701 ggtgtttgcg gacgccgtgc tcgaaatagc caggccgggc gacacgatct gggtgcacga
     2761 ctaccagctt atgctgctcc ccaggctcat ccgcgaccgc atgcccggcg tgaccgtggg
     2821 cttcttcctg cacatcccct tccccggcta cgagctattc cgcacgctgc cccgcaaatg
     2881 ggccaccgcg atacttgaag gcatgctggg ggccgacctg gtgggctttc acacccacga
     2941 gtacacgcag tatttctccc gcaccgtgtt gagggagctt ggctacgagc agaacctggg
     3001 cgaaatcctg gtgagcgacc ggctggtgaa ggtcgagacc ttcccgatgg gcatcgactt
     3061 caaccgctac cacgatgcgg tgtccacgcc ggaggtcgag cgggagcgca gcctgctgca
     3121 gaggtcggtg caggatacca agctggtgct ctccatcgac cggctcgact acaccaaggg
     3181 catcatcaac cgcctggaag gcttcgcccg cttcctcgag gagtaccccg agtggcacgg
     3241 acgtgtgatg atggcgctgg tgatagtgcc ctcgcgcatc ggggtcgagc ggtaccaggc
     3301 gacgaaggag cgcatcgagg agatagtggg ccgcaccaac ggcaggttcg gcagcatcac
     3361 ctggacgcct attacctacc agttcaagtc gatacccttc ccgctgctcg tcgccatgta
     3421 caacgtgagc gacgtggccc tcatcacccc cctgcgcgac ggcatgaacc tgatcgccaa
     3481 ggagtacgta gccgcccaca gcgggaacgg cggcgtgctc atcctgagcg agttcgctgg
     3541 cgcggccaag gagttgggtg acgctattat catcaacccc aacagccgcg aggagattgc
     3601 ccaggccatc agggaagccc tcgaaatgcc ccaggacgag caggaccggc gcatggagcg
     3661 tatgcaggac atcctcagac gcttcgacat cacctactgg gccgaggact tcatgggcca
     3721 gctacaggcc accagggagg cgcaggagaa gctcaaggcc cgcctgatga gcgatacggc
     3781 ccgcgagcgc atgctgggcg actatcgcta cgcgagcaag cggctgctgc tgatagacta
     3841 cgatggcacc ctggtgccct tcaacaagga cccccagcag gccaggcccg gcgacgacgt
     3901 agtgcaactg ctcgctcgct tgtgcgatac ccccggcagc caggtggtgc tcatcagcgg
     3961 gcgcgacaag gagacgctcg acaggtggtt tggcgacctg cccgtggcga tggtagccga
     4021 gcacggggta tggataaagc agccccacga gagcgagtgg acgatgtcgc aacccctcac
     4081 gagcgagtgg aagtcgcgca tccgccccgt gctggagatg tacgccaacc gcctgcccgg
     4141 ctcgttcctg gaggagaaag actactccat cgcctggcac taccgcggag ccgaccccga
     4201 cctggggccg agccgggcca aggagctgct ggaccacctg gtcaatttca cggccaatat
     4261 caacgtgcag gtgttgcagg gcaacaaggt aatcgaggtg aagacctccg gcgtcagcaa
     4321 gggcacggcg ggccgcacct ggctagacgg gggcgacttc gatttcgtgc tggctatcgg
     4381 cgacgactgg accgacgagc acctgttcat ggtgctaccc gaaagcgcct actccctgcg
     4441 cgtgggcgtg gtgcaatcgt acgcgcgcta caacctgcgc tcctacaccg aagtcacccg
     4501 cttgctcgaa gagctatccc acaacacccc cgccgaacct gcgctttatg gcgagcaaca
     4561 ggcacaaccc gtacacgcta gttaagagac ggaaccgcca agacgccaag gacgcgcaaa
     4621 ggttagtagg gtgttctgag caaggggacc agggtgctac atatgtaccc tggtcttttt
     4681 gcggggagta ctggaaacgg tagtcccagg tactagccaa ttagggccat gacttttatg
     4741 caccccctgt agtaagtagt gcccattcac ttagctcaac accgcaccta tacttagcgt
     4801 agggcctccc tgcgcgggtg gcatgggcac gtactcggct actttgccgg taaatgccac
     4861 atcagcctct cacatgccag gcgagccgga acatagatag accactggta cggcgctccc
     4921 ttccgt
//
LOCUS       DASRWZ010000771         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_747436, whole genome shotgun sequence.
ACCESSION   DASRWZ010000771 DASRWZ010000000
VERSION     DASRWZ010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747436"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VFH60_12210"
     CDS             complement(<1..1308)
                     /locus_tag="VFH60_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET6314589.1"
                     /translation="MTSVPRAEEAAKVTATPSEAPELSAPVEQAVATPSTTASYLSLL
                     LVYLPLGFSGIMMTLDLPIVNAVLNRFPNANTSVAALSVAFSLALVYEASHISMIDLS
                     TALSTDQRTFRMLQRFYLVMAGVLFVLASIIAFSPLYDLIVRGVMNIPPEVAEAARPA
                     VWAFLLWPVPIGWRRLCQGALIKHGHPKPVGAGGIVRMAALIVALTFFGWLGSSVVWI
                     EPAAIAVLAMLVSVTAEALAVQGWTSRLVKSMPEAIPGKPPPTWADIRRFFFPLSGTA
                     IMSTLVQPIMRAGIASAAVAWAAAPNDETVAVAVAAYQTAWSMAFLAFGPTLSMTQAS
                     IAWSGSSNEVVRERGPRVIMGVGVGLGALMALFAFTPLSEWIFSTVIQSPPQTAHLAA
                     EVARWLVPMPVLHAASFMLRGRLIAMHKPQAVRRAQLIDLLSIF"
     gene            complement(1344..1838)
                     /locus_tag="VFH60_12215"
     CDS             complement(1344..1838)
                     /locus_tag="VFH60_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6314590.1"
                     /translation="MTHDRHMIHFEIGNAVFNLRVVAVFVEGEYVLVHRAEPDDFWTL
                     PGGRAELGENTREAAAREMREELKVEAEVGKLLWVVEYFFDLPRHAYHEVAFYYRMHF
                     PLGASITPATPIFHGDDMGLPLIFRWVRLDELQGLRLYPTFLKTALCELPQTTQHVIL
                     RDDD"
     gene            2173..2907
                     /locus_tag="VFH60_12220"
     CDS             2173..2907
                     /locus_tag="VFH60_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HET6314591.1"
                     /translation="MDVPLKPYKREFDHSYALGVFPTLELVEAQPRHVLKVLVSSKGE
                     RNEGLRKLERLCERAGIRLETNDRAVERLAQKENTFAVGVFRKYASGLQAASNHIVLV
                     NPGDMGNLGTIARTMLGFAVADLAIVRPAADMFDPRVVRASMGAIFRLRCRYFDTFEQ
                     YREEAGERSLYPFMTGGDLMLDKTAFNPPFSLIFGNESAGLPPEYRATGTPVTIRHTA
                     QIDSLNLPMSVGIALYEATKHAANFT"
BASE COUNT          568 a          901 c          968 g          523 t
ORIGIN      
        1 gaatatcgag agcaggtcta tcagttgggc gcggcgcacc gcctggggct tgtgcatggc
       61 tatcagcctg ccccgcagca tgaaggaggc cgcgtgcagc accggcatcg gcaccagcca
      121 gcgggctacc tcggccgcaa ggtgagcggt ctgcggcggc gactgtatga cggtgctgaa
      181 tatccactcg gatagcggtg tgaaggcgaa cagggccatc aacgcgccca gccccacgcc
      241 tactcccatg atgacgcgtg gaccccgctc gcgcaccact tcgtttgagc tgcccgacca
      301 ggcaatcgaa gcctgcgtca tgctcaatgt cgggccgaag gcgaggaagg ccatgctcca
      361 ggcggtctgg taggcggcca cggccacggc taccgtctcg tcgttggggg cagcggccca
      421 ggccacggca gcggaggcga taccagcccg catgatgggc tgcaccagcg tgctcataat
      481 ggccgtgcca ctgaggggga agaagaagcg gcggatgtcg gcccaggtgg gcgggggctt
      541 gcccggtatg gcttcgggca tcgacttcac caggcggctg gtccagccct gcaccgccag
      601 cgcttccgcc gtgacgctca ccagcatcgc gagcaccgct atggccgcag gctcgatcca
      661 caccacgctg gagcctaacc agccgaagaa ggtcagcgcc acgattagcg ccgccatcct
      721 gactatgccg cccgcgccca ccggcttggg gtggccgtgc ttgatgagcg cgccctggca
      781 cagccgccgc cacccgatgg gcacgggcca cagcaggaag gcccacacgg cgggccgcgc
      841 ggcctcggct acctccggcg gtatgttcat cacgccccgc acgatgaggt cgtacagggg
      901 ggagaacgcg atgatcgatg cgagcacgaa cagcacgccc gccatgacga ggtagaagcg
      961 ttgcagcata cggaaagtgc gctggtcggt ggagagggcg gtcgagaggt cgatcatgga
     1021 gatgtgcgag gcctcgtaga caagcgcgag ggagaaggcc acgctgaggg ccgccacgga
     1081 cgtgttggcg ttcgggaagc gattgagcac cgcgttcacg atgggcaggt ccagcgtcat
     1141 catgatgccg ctgaagccga gcggcaggta gacgaggagc agcgacaggt agctagcggt
     1201 ggtcgagggg gtggcgaccg cttgctccac gggtgccgac agttcggggg cttcgctggg
     1261 ggtggcggtg actttggcgg cttcctcggc ccgtggtacg gaggtcatgc gaaattatac
     1321 aacatgaggc atgaccgggg cgttcagtcg tcgtcccgca ggattacgtg ttgcgtcgtc
     1381 tgcggcaact cgcataaggc ggtcttgagg aaggtgggat ataggcgcag tccttgcagt
     1441 tcgtccaggc gcacccaccg gaagatgagg ggcagcccca tgtcatcgcc gtggaagatg
     1501 ggcgtggcag gagtgatgct cgcgccgagt gggaagtgca tcctgtagta gaaagccacc
     1561 tcatggtagg cgtgcctcgg caaatcgaag aaatactcga ctacccacag cagcttaccc
     1621 acctcggctt cgaccttcaa ctcctctcgc atctctctgg ccgcggcctc cctggtgttt
     1681 tcccctagtt cggcgcgccc gcccggcagc gtccagaagt cgtcaggttc ggcccggtgg
     1741 accagcacgt actcgccctc tacgaacacg gctaccacgc gcagattgaa gactgcgttg
     1801 ccgatttcga agtggatcat gtgtctgtcg tgggtcattt tgcaccctgc cgatgtctac
     1861 acctgccggg tggttaacta aaagacagag ttccggcagg acgagaaagc ccggtgttca
     1921 tagcaatacc tccacgggcg aactaaggat gataatatca tagcatcttc cgcgagggta
     1981 gtggccgtgt gatggaagta gcgcggtacg gctgtgttaa agtagctctg cagtaccaaa
     2041 acttcccctg tcctcctgaa cccttcggct gtactcagga tgacaggggc cgcatagctt
     2101 gacagcccac ttacccttcc tgtataccgc tagcagccgc cccgtctccc atacccggca
     2161 tgacaccgcc ttatggacgt tccgctgaag ccttacaagc gagagttcga ccattcttac
     2221 gcgctgggcg tgttccccac gctggagctt gtggaggctc aacctcggca cgttctgaag
     2281 gtgctggtaa gctcgaaagg agagcgtaac gagggcctgc ggaagctgga gcggctttgc
     2341 gagcgggcgg gcatcaggct tgagacgaac gaccgggcgg tggagcggct ggcgcagaag
     2401 gagaacactt tcgcggtagg ggtgtttcgc aagtacgcct ccggcctgca agcggcgagc
     2461 aaccatatcg tgctggtcaa ccccggcgac atgggcaacc tgggtactat agcgcgcacc
     2521 atgctcggct tcgcggtggc cgacctcgct atcgtgcgcc ccgccgccga catgttcgac
     2581 cccagggtgg tgcgggcctc gatgggcgct atcttccggc tgcgctgccg ctacttcgat
     2641 acgttcgagc agtaccggga ggaggcgggc gagcggtcgc tgtacccctt tatgaccggg
     2701 ggcgacctga tgttggacaa gaccgcgttc aacccgcctt tctcgctgat attcggcaac
     2761 gagtccgcgg gcctgccccc tgagtaccgc gcaacgggta cgcctgttac catcagacac
     2821 accgcgcaaa tcgactcgct caatctgccc atgtcggtgg gcatcgcgct ctacgaagcg
     2881 acaaagcacg cagcgaactt tacataacgg gagacgcatg tgagcaagaa gcttcactac
     2941 tatatcgtgg acgtgtttac
//
LOCUS       DASRWZ010000772         4406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1227393, whole genome shotgun sequence.
ACCESSION   DASRWZ010000772 DASRWZ010000000
VERSION     DASRWZ010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227393"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFH60_12225"
     CDS             <1..388
                     /locus_tag="VFH60_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314592.1"
                     /translation="NGTITQRGILNTLGMGTGEAAVLNLTDGDLSVDIVSLGDDSGSS
                     LLETNLSLKPTDSNSFTAIRPGRYRLTFTASVGGAASGTCSLQMGSGDLYQFVALNEG
                     VVVTSEKYTPTTAADMNMATSSLCKK"
     gene            407..931
                     /locus_tag="VFH60_12230"
     CDS             407..931
                     /locus_tag="VFH60_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314593.1"
                     /translation="MRDKRPRDLRPRLMALLLLLLGVLLVGCSSIGVENTSTDQTARV
                     TMYLPDRSGSSSFTLGPGQSGSELSTESGLVVILVMPNDEYIKQLEEMKKYFEAVLRV
                     PTFTQQDYKDATTRVKEFNEKIKQASVGGNSCYVKVAENVDVVASLAWDEPNATWNIT
                     CVTRKVETESSPSD"
     gene            1039..1698
                     /locus_tag="VFH60_12235"
     CDS             1039..1698
                     /locus_tag="VFH60_12235"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HET6314594.1"
                     /translation="MRRYRLSGMAGRLVGVLALFSGAALLTHIVWRVSLPLALAAAVL
                     VLVFSAATLWRRATPTQRVMLARLVKVGAASGLVATVSYDAAKYALSQFDPSPYNPFE
                     AIRIFGTLLVGQPASELAIYAAGASFHLLNGVSFGVAYCFLFRRQGIVAGMAWGLFLE
                     MFQLTLYPGWLNIRLYSEFAQISAISHVVYGLVLGKVCQVTLARSQGTDLTARGAEHG
                     Q"
     gene            1688..3058
                     /locus_tag="VFH60_12240"
     CDS             1688..3058
                     /locus_tag="VFH60_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314595.1"
                     /translation="MGNNPNWGAGPGMPVPPQPQQPYPQQPYPQQPFPQPPPPPPSMQ
                     PQQPMPSSQSPDERLERFWYVKAPAWLDDWFPQIAKAISDGAWTTAWRGVGAYAPVVG
                     FALGLLCRIFFPQIYSTFSESLLFMMIVIAVSLLSGTMGAALLAGYIVEDLLVGNRAS
                     VNTGFFPAEMPPEGLSGLPGVLGGKLVSYLLLSIPTITIPLLVRQLAPSIKLHTITDP
                     NSRTFALAGLHAAIAGVLIFLWAQSVVVLLRPLFTWVGAVPTFQAINPVQAQWSFLVG
                     VAVVAAAGRVILERMIARTAPRAAVAATLQRQRFTGEGQGILEKVPGLVRVVIPPVVI
                     AMLLAGTYDGWLDAIIVAAVAAVLGLWRSRLIRIIPLPSQWSLTIRRIPALIRLAAAP
                     FIGYWLATIVITPMWTNTAQGLRPVMLGSLVTLIVFYVLFPPLPVVPGPTPQPQPQPQ
                     PQPTRY"
     gene            3060..4391
                     /locus_tag="VFH60_12245"
     CDS             3060..4391
                     /locus_tag="VFH60_12245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6314596.1"
                     /translation="MLLSGLEIPPLFATLAVLLDNCSSLFDCWDTLGAAAAAAVGAGA
                     AAVFVGGLLLGPGEAPGIVEEGVPPQVAASASEPQPASPGGGAPTTVAQAPPQASPHG
                     VQPQAPPQVAPQAPPQAPPQATAPTAPTAPTAPTAPQGTPVFGGEVQPGAIAASAPAS
                     PGTPSPAFEAPPIGASPTAPAAPPAAPPGAPPVAPPVAPPVAPPVVPPAADTALAAGL
                     AAGVAPVVSTLKAVLGSRAGGSGAGSDAAGQDEGDQDDLDTCPEADASLFGLPDIITR
                     QDCPCTRPGMPPYYAGLDFTSNDDHGRPLELPFEAGVTGEVTYVGGAFNSVQVTLDNG
                     NRIQFLHASQVLVQLGQQVEPTTVLGRTGGTGPYGPNQYEIHLHVQAFDRHGDLIDPD
                     CALSGGENRRLRRNVVPPATQATQATSAPPEAPREAFTTPGTTAVLSGTQP"
BASE COUNT          772 a         1525 c         1365 g          744 t
ORIGIN      
        1 aaacggcacc atcacccagc gcggcatact caacacgctg ggcatgggga ccggcgaggc
       61 cgccgtgctc aacctgaccg acggcgattt gagcgtcgat atcgtttcgc tgggcgacga
      121 ctccggttct tcgctgcttg aaactaacct gtcgctcaag cccaccgaca gcaacagctt
      181 caccgcaatc aggccggggc gctaccgcct caccttcacg gccagcgtag gcggcgcggc
      241 gagcggcacg tgctctttgc agatggggag cggcgactta taccagttcg tcgcgttgaa
      301 cgagggcgtg gtcgtcacca gcgaaaagta cacgccgacc acggcagccg atatgaacat
      361 ggcaacgtcc tcgctgtgca agaaatagca gatgggaggt agcaacatgc gcgacaaacg
      421 gccccgcgac ctgagacccc gcctcatggc gctgctgctc ctgctcctgg gcgtgctgct
      481 cgtggggtgt tccagcatcg gggtggagaa cacctccacc gaccagaccg cccgtgtgac
      541 gatgtacctg cccgaccgta gcggctccag ttcattcacg ctcggccccg gccagtccgg
      601 tagcgagctt agcaccgaga gcggcctggt ggtcatcctg gttatgccca acgacgagta
      661 tatcaagcaa ttggaggaga tgaagaagta tttcgaagcc gtgttgaggg tgcccacctt
      721 cacccagcag gactacaagg atgccacgac cagggtaaaa gagttcaacg agaagataaa
      781 acaggccagc gtgggcggca actcttgcta cgtcaaagtc gcggagaacg ttgacgttgt
      841 agccagcctg gcgtgggacg aacctaatgc cacctggaat atcacctgcg tcacgcggaa
      901 ggtagagacg gagagcagcc catcggactg accaaagatg tgtgcacgag gtcacacgcg
      961 aggcacaagc aaagcagtga gccagaggaa accaaagagt agcttaggtg ggtgaagccg
     1021 tagatgatgt cgcgcctggt gagacgctat agactgagcg gcatggctgg caggctggtg
     1081 ggagtgctgg cgctcttttc cggggcggcc ctgctgacac acatcgtctg gcgggtgtcg
     1141 ctacccctcg cccttgccgc cgccgtgctc gtgctcgtct tcagtgccgc gaccctctgg
     1201 cgcagggcca ccccaacgca acgcgtcatg ctcgcccggc tggtgaaggt cggggcggct
     1261 tcgggcctgg tagccaccgt cagctacgac gccgccaagt acgccctgtc ccagttcgac
     1321 ccctcgcctt acaacccgtt cgaggcgata cgcatattcg gcacgctgct cgtgggccaa
     1381 ccggcctcgg agctcgccat atacgcggcg ggggccagtt tccacctgct gaacggggtg
     1441 tcgttcggcg tggcctattg tttcttgttc aggcggcagg gcatagtagc gggtatggcg
     1501 tggggcttgt tcctcgaaat gttccagttg accctctacc ccggttggct gaacatccgc
     1561 ctctactccg aattcgcgca gatttcggcc atatcgcacg tcgtgtacgg cctggtgctt
     1621 ggcaaagtgt gccaggtcac actagcccgc tcgcagggca ccgatctaac agcaagggga
     1681 gcagaacatg ggcaataacc cgaactgggg cgcggggccg ggtatgccgg tgccgccaca
     1741 gccccaacaa ccatacccgc aacaacccta tccgcaacag ccgtttccgc agccaccgcc
     1801 gcccccgccc tcaatgcagc cgcaacagcc gatgccctcg tcacagtccc cggacgagcg
     1861 gttggagcgg ttctggtacg tcaaggcccc cgcgtggctg gacgactggt tcccgcagat
     1921 agccaaagcc atctcggatg gggcgtggac tactgcctgg cgcggagtgg gcgcgtacgc
     1981 ccctgtggtg gggttcgcgc tgggcctcct ctgccgtatt ttcttcccgc agatatacag
     2041 caccttctcc gagtcgctcc tgttcatgat gatagtgata gccgtctccc tgctgagcgg
     2101 cacgatgggc gcggccctcc tggccggcta cattgtggaa gacctgctcg tggggaaccg
     2161 ggccagcgta aataccggct tcttcccggc ggagatgccc ccggaagggc tgtcggggct
     2221 gccgggcgtg ttgggaggca aactggtcag ctacctgttg ctctcgatac ccaccatcac
     2281 catcccgctg ctagtgcggc agttggcccc cagcatcaag ctgcacacca tcaccgaccc
     2341 gaatagccgc accttcgccc tggccgggct gcacgccgcg atagccgggg tgctcatctt
     2401 cctgtgggcg cagtcggtgg tcgtgctcct gcgcccgctc ttcacgtggg tgggcgcggt
     2461 gcccaccttc caggcgatta accccgtgca ggcgcagtgg agcttccttg tgggcgtagc
     2521 agtggttgcc gccgcgggcc gcgtgatact ggagcgcatg atcgcacgca ccgcccccag
     2581 ggccgccgtg gccgccacgc tgcaacgcca gcgcttcacg ggcgagggcc agggcatact
     2641 tgagaaggtg ccgggcctgg tgcgcgtcgt catcccgccc gtggtgatag cgatgctgct
     2701 ggcgggcacc tacgacggct ggcttgatgc cattatcgtg gcggccgtgg ccgcggtact
     2761 ggggctgtgg cgctcccgcc tgattcgcat tatacccctg ccctcccaat ggtcgctcac
     2821 gatacgtcgc atccccgcgc taatccggct ggcggcggcc cctttcatcg gctactggct
     2881 ggctactata gtaatcacgc cgatgtggac caacacggcg cagggcctgc gcccggtgat
     2941 gttggggtcg ctggtgactc tgatcgtctt ctacgtgctg ttcccgcctt tgccggtagt
     3001 ccccggcccc acaccccaac ctcaaccgca acctcaacca caaccaacga ggtactagca
     3061 tgcttctctc cggcctcgaa atcccaccgc tgttcgcgac gctggcggtg ctcctggaca
     3121 actgtagctc attattcgac tgctgggaca cgctaggtgc ggccgcggcg gctgccgtgg
     3181 gagcgggcgc ggcggccgtg ttcgtgggtg gcctgctgct cggccccggc gaggcgcccg
     3241 gcatagtgga agaaggcgtg ccgccccagg tcgcagcctc cgcgagcgaa ccccaaccgg
     3301 cctcgcccgg cggtggcgca cctaccacgg tagcgcaagc gccccctcaa gcatcacccc
     3361 acggcgtgca gccccaggca ccccctcaag tagctcccca ggcaccgcca caagcgcctc
     3421 cccaagctac ggcacctaca gcacctacag cacctacagc acctacagct ccgcaaggga
     3481 cgccggtgtt cggcggcgag gtacaaccgg gggcgatagc tgcttctgcg cctgccagcc
     3541 cgggcacgcc cagccccgct ttcgaggctc cgcccattgg ggcatcaccc accgccccgg
     3601 ccgcaccccc ggccgcgcct cctggggcac ctcccgtggc acctcccgtg gcacctcccg
     3661 tggcacctcc tgttgtgccc ccagcggccg acaccgcgct tgctgccggt ttggcggcgg
     3721 gagtggcacc cgtggttagc accctgaagg cggtactggg tagcagggcc ggcggttcgg
     3781 gcgcgggaag cgacgccgcc ggccaggacg agggggacca ggacgacctc gacacctgcc
     3841 ccgaagcgga cgcctcgctg tttggcctgc ctgacataat cacccgccag gactgcccct
     3901 gcacccgccc gggcatgccc ccctactacg cgggcctcga cttcaccagc aacgacgacc
     3961 acggtcgccc cctggaattg ccatttgaag ccggggtgac gggagaagtg acctacgttg
     4021 gcggcgcgtt caactcggtg caggtgacgc tcgacaacgg caaccgcatc cagttcctcc
     4081 acgcctcgca ggtgctcgtg cagttgggcc agcaggtaga acctaccacc gtgctcggca
     4141 ggacgggagg caccggcccc tacggcccca accagtacga gatacacctg cacgtccagg
     4201 cgttcgaccg gcatggcgac ctgatcgacc ccgattgcgc cctctccggt ggggagaacc
     4261 gccgcctcag aaggaacgtc gtgccacccg cgacacaggc gacacaggcg acctccgccc
     4321 cacccgaagc cccccgcgag gcattcacca cgccgggtac taccgctgtg ttgagtggca
     4381 cgcagccctg accctacgcc ccacag
//
LOCUS       DASRWZ010000773         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_953091, whole genome shotgun sequence.
ACCESSION   DASRWZ010000773 DASRWZ010000000
VERSION     DASRWZ010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953091"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1368
                     /locus_tag="VFH60_12250"
     CDS             85..1368
                     /locus_tag="VFH60_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314597.1"
                     /translation="MATSNEQLTTNNPPMTDIRPQQAVDQALPDPDDFLELEGDEFSA
                     AFGESLDATLDLATWQPGEDIGALYERLEREIAGAVDQEDRIRERIRAELFPYVFSRN
                     GAPPGAGCYRVDLPTLERVHRGLLFTGAVEACDGTSLIHDSLALTIAQIGVSLVSYRG
                     DQGTWVQRLFRRDLRVHGLDPIGEAMSMLEKRHRRAVAEDGNSDRLSDLSRRGIMAYA
                     ERAILLRKSSAMWRVGHGNPVSLELLSGAGLVIDGEMPLLRQSIAVWRELLAGHKKWI
                     YVPSAPANRVLLTLGNALYPLEYALVDTPTRTLRDFVENNLPQSKYIYPRDEARTFVE
                     EVAPQLVIGVYRASGAAPPYMFYAHRDYAHEAAMIVMADSALQEHRGFPMLIDLADSV
                     CRSTFGLDTFSAAIREAYVQAGVPIRYLGEREMRP"
     gene            1573..2067
                     /locus_tag="VFH60_12255"
     CDS             1573..2067
                     /locus_tag="VFH60_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314598.1"
                     /translation="MDDRSVYDYAFLGEVSYWFETSDELFVAVRYAHAGGDRDYLFAD
                     SFAQILDLANILPPTTDIVVFRQKQLPYRGTADESLLSAALAGIPDGADWLLLTRRRE
                     GEQDFTSKSGSTHEGLRGAFGEFRGKYVAVGAAPVFSGADGEDVQSALVPFPDGSVQR
                     AEAY"
     gene            complement(2228..2515)
                     /locus_tag="VFH60_12260"
     CDS             complement(2228..2515)
                     /locus_tag="VFH60_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314599.1"
                     /translation="MAVEKHPIPFDEFAGDVAGFFERVVHGHETLLVEKAKDEIVVVK
                     AARSTRRARVRTSSRPSVNRDAFLATAGGWKDIVDTDKLVEHIYKSRSICL"
BASE COUNT          485 a          772 c          876 g          489 t
ORIGIN      
        1 ctgatccttc gctacgctca ggatgacaag gggggctggg cactcccgct ccatcggtga
       61 tgctgttatc agcacctgct cctattggcg actagcaacg aacaactaac aactaacaac
      121 ccgcccatga ccgatattcg cccacaacag gccgtggacc aggcgctccc cgacccggac
      181 gacttcctgg agttggaggg ggacgagttc agcgcggcct ttggcgagtc gctcgatgcg
      241 acgctggacc tggcgacctg gcagccgggc gaagacatag gggcgctgta cgagcggttg
      301 gagcgggaaa tcgcgggcgc ggtggaccag gaggaccgca tccgggagcg catccgggcc
      361 gagttgttcc cctacgtgtt cagccgcaat ggcgcgcccc ccggcgcggg ctgctaccgc
      421 gtggacctgc cgacgttgga gcgggtgcat cgcggcctgc tgttcacggg ggcggtggag
      481 gcatgcgacg gtaccagcct catacacgac tcgctggcgc tgacgattgc ccagataggg
      541 gtgtcgctgg tgtcgtaccg gggcgaccag gggacgtggg tgcagcggct cttccggcgc
      601 gacttgcggg tgcacggcct cgacccgata ggcgaggcga tgtcgatgct ggagaagcgg
      661 caccgccggg cggtggcgga ggacggcaac agcgaccgcc tcagcgacct gagccgccgg
      721 ggcatcatgg cctacgccga gcgggcgata ttgctgcgca agtcgtcggc tatgtggcgc
      781 gttgggcacg gcaacccggt tagcctggag ctgctgtcgg gggccgggct ggtgatagac
      841 ggcgagatgc ccctgttgcg ccagagcatc gcggtgtggc gggagttgct ggcggggcac
      901 aagaagtgga tttacgtgcc gagcgccccc gccaaccgcg tgctgctgac gctgggcaac
      961 gccctctacc cgctcgaata cgccctggtg gacacgccga cccgcacgtt gcgcgatttt
     1021 gtggagaaca acctgccgca gagcaagtac atctacccgc gagacgaggc aaggacgttc
     1081 gtggaggagg tggccccgca actggtgata ggggtatacc gggcgtcggg ggcggccccg
     1141 ccttatatgt tctacgccca ccgcgactat gcccacgaag cggctatgat cgtcatggcc
     1201 gacagcgcgt tgcaggagca ccggggcttc cccatgctga ttgacctggc cgactcggtc
     1261 tgccgcagca cgttcggcct cgacacgttc agcgcggcta tacgcgaggc gtacgtgcag
     1321 gctggcgtgc cgatccggta tttgggcgag cgcgagatga ggccgtaacg tgactaatgt
     1381 aacgtgacga atgctgaggg tgtattatag cgcctgtcgg gagcgtcctg gcaccgaggg
     1441 aaaagcgaag tggaaagggt atgcctataa gtagcagctt cacttttgct ccgtgtccgt
     1501 agccggtttg tggctgcgcc gggcacctct actttgcaag ccgcagatag gactatagcg
     1561 aggcaccaga gcatggatga caggtcggtc tacgattacg cgttcctggg tgaggtctca
     1621 tactggttcg agacgagtga cgagctgttc gtcgccgtgc gatacgcgca tgcgggcggg
     1681 gaccgggact acctgttcgc ggactcgttc gcgcagatcc tcgacctggc gaatattcta
     1741 cctccaacca ccgacatagt ggtcttcagg cagaagcagc taccttaccg gggcacggct
     1801 gacgaaagcc tgctcagcgc cgcgctcgcg ggcattcccg acggcgcgga ctggttgctg
     1861 ctgacccgcc gcagggaggg cgagcaggac ttcacctcta agagcggctc cacgcacgaa
     1921 ggcttgagag gggcgttcgg ggagttcagg gggaagtacg tggcagttgg tgcggccccg
     1981 gtgttttcgg gggctgatgg ggaggatgtg cagtcggcgc tggtgccgtt tccggatggt
     2041 agcgtgcagc gggcggaggc ttattgaagt agcctgcctt acgcccggcg gttgaaaccg
     2101 cgtgctacac ttacaaagtc cgccttcgcg gactgtctca gaacaggaaa gtccccgtag
     2161 ggggacttcg taaaagtagc acgcggtttc aaccgccggg cggtgcattg cacaggagca
     2221 cgggtggtca caaacagata cttcgactct tatatatgtg ttcaacaagc ttgtctgtgt
     2281 caacgatgtc tttccagccc ccggcagttg ctagaaaggc atctctgttg acggaaggcc
     2341 gactggacgt gcgaaccctg gctcgtctgg tggagcgtgc cgcttttaca acgacaatct
     2401 cgtcctttgc cttctccacc agcaatgtct catgcccatg cacgacgcgc tcgaagaatc
     2461 cggccacatc accggcaaat tcgtcaaagg gtattgggtg tttctctacc gccatgatag
     2521 ttctccttta ctgaattgta tcaccgtcac gcaaggaagt gcgcccgttg aacgacggca
     2581 atcccacccc caatccacaa accggctccc ccgaccgctg gg
//
LOCUS       DASRWZ010000774         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_747969, whole genome shotgun sequence.
ACCESSION   DASRWZ010000774 DASRWZ010000000
VERSION     DASRWZ010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747969"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="VFH60_12265"
     CDS             complement(<1..1375)
                     /locus_tag="VFH60_12265"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET6314600.1"
                     /translation="MARLTYFGLYALQHRGQESAGIATTGGNDLMIRARMGLVSTAFD
                     EESLAGLTGHAAIGHTRYSTTGSSKICNAQPLRLHDPALGWLALAHNGNVTNANPLQA
                     ELQSQGVQVETTSDSEVIGKLAIASHGTNWVTKFRRAMSRLTGAYSLALLTPTHLIAV
                     RDPMGIRPLCIGRLPGTAPVGEHSKHKGEPIAHSPLRSAQSKSGGWVIASESCALMTI
                     GAEFIREIEPGEIVTIGPEGLTSYGDVPSVAQAMCVFEYIYFARPDSVINNVSLYTAR
                     QSMGRELAREHPADADIVIGVPDSATPAAIGFSLESGIPFTEGLIKNRYIGRTFIQPH
                     QHLRERGIGLKFNPLPGVLRGKRVVVVDDSIVRGTTTRPIVELLRRAGATEVHVRIHS
                     PAMSHPCYLGVDTARREELIAHRMSVPDIARHIGADSLGYLSLPGLFRAVGLTSERLC
                     SGCFTGDY"
     gene            complement(1635..>3548)
                     /gene="purL"
                     /locus_tag="VFH60_12270"
     CDS             complement(1635..>3548)
                     /gene="purL"
                     /locus_tag="VFH60_12270"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET6314601.1"
                     /translation="LNSLRFGPLSDPHTRYLFGGVVAGIGGYGNCIGVPTVGGEIAFD
                     PSYAGNPLVNAMCVGLIRSDSIMRAVASGAGNPVLLVGADTGRDGIHGATFASDTLGE
                     DALQDRPAVQVGNPFLEKCLMEACLEVLQLDNVVVGMQDLGAAGLTSSSVECAARAGM
                     GIEIDLAHVARRERGMNAYEIMLSESQERMLVIVRQGREAEAQAVFSRWGLHSDVIGS
                     VTGDGMVRVYDGDVLAAELPATLLADEVPIRYVEGVPDPELYELQHPDWDAILPQENP
                     GTRYDNALLDVLASPNVASKARVFRTYDHTIGSTTVLQPGEADAAVLRIKGTSKGLAL
                     TTDCNGRYCYLDPYGGGVHALAEAARNLSCVGAEPLAVTDCLNFGNPERPHVFYQMES
                     AVNGIADACNALGVPVVSGNVSLYNETNGVAVLPTPVIGMLGLLEDVERRAGIAFREG
                     MAIGLLWAKHAGDEPYAGGGHLLGASQYAETCLGLKVGMPPPVDLVAERAVQHVCRES
                     IRLGIVDIAHDCSNGGFAVALAEGCIAGDVGASIHLDELEKIHPSLRSDVLLFAEEPS
                     RIIVALPLSNWDGLMELASSMEVSLCRLGDTWGETLTVERGEHQLVSLPMLDVAAAWQ
                     GPGVRDQGSGTRD"
BASE COUNT          648 a         1077 c         1155 g          668 t
ORIGIN      
        1 ggtaatctcc cgtgaagcag cctgagcaga gcctttcgct ggtaaggcct accgcgcgga
       61 agaggccggg caggctgagg tagccgagcg agtcggcccc gatgtggcgg gctatgtcgg
      121 gcacgctcat gcggtgggcg attagctcct cccggcgggc ggtatccacg cccaggtagc
      181 aggggtggct catcgcgggc gagtggatac gaacatgtac ctcggtggcc ccggcacggc
      241 gcagcaactc cacgatgggg cgggtggtgg tgccgcgcac tatggagtcg tccacgacca
      301 caacgcgctt gcctcgcaat acccccggca aaggattgaa cttcaggccg atgccgcgct
      361 cccgcaggtg ctggtggggc tggatgaagg tgcggcctat gtagcggttc ttgataagcc
      421 cctcggtaaa ggggatgccc gattcgaggg aaaaaccgat ggcggcggga gtggccgagt
      481 cgggcacgcc tatgactata tcggcgtcgg ccgggtgctc ccgtgcaagc tctcgcccca
      541 tgctctggcg tgccgtgtag agcgatacgt tgtttatcac gctgtcgggc cgagcgaagt
      601 agatgtactc gaatacgcac atcgcctgcg ccacgctcgg cacgtcccca tatgaggtaa
      661 gcccctccgg cccgatggtc actatctccc caggctcgat ctcgcgtatg aactccgcgc
      721 ctatcgtcat cagtgcgcac gactcggagg cgattaccca gccgcccgac ttcgattgcg
      781 cactcctaag aggggaatgg gcgataggct cgcctttgtg cttgctatgc tcgccaacgg
      841 gtgccgtgcc gggcagcctg cctatgcaaa gggggcgtat acccataggg tcgcgcacgg
      901 cgataaggtg ggtaggcgtc aatagcgcga gggagtaagc ccccgtcagg cgggacatgg
      961 cgcggcggaa cttcgtcacc cagttagtgc cgtgcgaggc tatagccagc ttgcctatta
     1021 cttcactgtc ggaggtagtc tcgacttgga cgccttgcga ctgaagttca gcctgtagag
     1081 ggttggcgtt ggtgacgttg ccgttatgtg ccagggccag ccagccgagc gcggggtcat
     1141 gtaagcgtaa gggctgggca ttgcatatct tagaggagcc ggtggtcgaa tagcgggtgt
     1201 ggcctatcgc ggcatgtccg gtcagacctg ctagcgactc ctcgtcgaac gcagtggata
     1261 ccaggcccat acgcgcgcga atcatgaggt cgttgcctcc ggtggtagca atcccagccg
     1321 actcctgccc ccggtgctgc aaagcgtaaa ggccaaagta ggtcagacgg gccacatcct
     1381 ccccaggcgc gtatacgcca aagacgccgc actcttcgtg cattttgtcc gaggggtcag
     1441 gggtcaggga ccagggatca gggactaggg gcgaggggcg aggggcgagg ggcgagggat
     1501 cgggagttgg aggtgaggag ataacagcat caacagtatg cgaaattgcc ggatcaacgc
     1561 tatcttgatg cctattcgtg ttgctcatta tgcactccga cctttgctag tagcgtctcg
     1621 cggtccgctg attactaatc tctggtccct gacccctgat ccctgacccc tggcccctgc
     1681 cacgcagcag ccacgtcgag cataggcagg ctcaccagtt ggtgttcgcc ccgctctacg
     1741 gtgagcgttt ctccccatgt gtcgcccaga cggcataggc ttacctccat cgaggaggca
     1801 agctccatca ggccgtccca attgctgagg ggaagcgcca caattatacg ggatggctcc
     1861 tcggcaaaca agagcacatc cgagcgtaag cttgggtgga ttttctccaa ttcatccagg
     1921 tggatagacg cgccaacgtc gcccgctatg cacccttcgg ccagcgcaac ggcaaacccg
     1981 ccattgctgc aatcgtgggc aatatctact atgcccaggc gtatgctttc tctgcatacg
     2041 tgctgcaccg cacgctcggc taccaggtca accggcgggg gcatgccgac cttcaatccc
     2101 aggcaggtct cggcgtactg gctcgcgccc aggagatgcc cgccgccagc gtatggctca
     2161 tcgccagcgt gcttcgccca gagtaagcct atcgccatcc cttcgcggaa ggcgatgcct
     2221 gcgcggcgct ccacgtcttc cagcaggccg agcatgccta tgacgggagt tggcagcaca
     2281 gccacgccgt tggtctcgtt gtagagcgac acgttgccgc tcaccacagg cacgcccagg
     2341 gcgttgcaag cgtccgctat gccgttcacc gcactttcca tctggtagaa gacgtgaggg
     2401 cgctcagggt tgccgaagtt gaggcaatct gtcacggcta gcggctccgc gcccacgcag
     2461 gagaggttgc gcgcggcttc ggctaaggca tgcacgccgc ctccgtaagg gtcgaggtag
     2521 cagtagcgcc cattacagtc ggtagtgagg gcgagtcctt tggatgtgcc cttgatgcga
     2581 agcacggcag cgtccgcctc gccaggttgg aggacggtag tgctgcctat cgtgtggtcg
     2641 taggtgcgga agacgcgcgc tttgctcgcg acgttgggag atgccagtac gtccaggagg
     2701 gcgttgtcat agcgcgtacc ggggttctct tggggcagaa tggcgtccca atcaggatgc
     2761 tgcaattcat acagttcggg gtcaggcacc ccctcgacgt agcggatggg cacttcgtcg
     2821 gccagcaggg tagcgggcag ttcggcggct agcacgtcgc catcgtagac gcgcaccata
     2881 ccgtcgccgg taacgctgcc aatcacgtcc gagtggaggc cccaccggct gaataccgcc
     2941 tgggcttcgg cctcgcgccc ctgtctgact ataaccagca tgcgctcctg cgactcggag
     3001 agcattatct cgtaggcatt catgccgcgc tcgcgccggg cgacgtgcgc caggtctatc
     3061 tcgatgccca tgcctgcgcg ggcggcgcac tctacggagg aggaagtgag gcccgccgcg
     3121 ccgaggtctt gcattccgac tacaacgttg tcgagttgga gcacttccag gcaggcttcc
     3181 ataaggcact tttcgaggaa ggggttgccc acctgcacgg ctgggcggtc ctgaagcgcg
     3241 tcctctccaa gcgtatcgga ggcgaaagtc gcgccgtgta tgccgtcgcg cccggtgtcg
     3301 gcccctacca gcagcacggg gttacccgca ccagaggcca ccgcgcgcat gatgctatcg
     3361 gagcgtatga ggcccacgca catcgcgttc accaggggat tgcccgcgta tgatgggtcg
     3421 aaagctatct cgccgccgac ggtgggcacg cctatgcaat tgccgtagcc gccgatacct
     3481 gccactacgc cgccgaagag gtagcgggtg tgaggatcgg agagggggcc gaagcggaga
     3541 gagttcag
//
LOCUS       DASRWZ010000775         6576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_199887, whole genome shotgun sequence.
ACCESSION   DASRWZ010000775 DASRWZ010000000
VERSION     DASRWZ010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199887"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..2123
                     /locus_tag="VFH60_12275"
     CDS             414..2123
                     /locus_tag="VFH60_12275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6314602.1"
                     /translation="MQRSEPGSSDVNLPRRDAPNVNRSRVLIVLSALSLALVVVAVLA
                     AWVWPSVAGRASGSTPDVAGTGTSSAADAASAPGDVALVQASSTRVPPTQVPPTATPT
                     LTPYTGDPVAARPVPSLGPGMRYFTETGHLVSGDFYSFYRSVQKSADLFGLPLTEAFA
                     QKLPDGGVYMVQYFERARLELHPDLPASQRVQIGALGAAILQGRSFDRVSPVQNSSTR
                     AYFRETGHTIQGGFFEFWLANGGLRLFGLPLSEEMHEGGLTVQYFERARFEYYPGLVG
                     TPYAIQVSPVGYAALKQSSFNIPMGTMLRFNPPALAEGHTSIVEVAASPGVSVTAQYE
                     GRTLYFKLDEGREVAWAVLGAVPFQDVGPRQVTFYFEHGDGGRRVVTRTLEITPYRFP
                     SETVRFDPETAALLDPAYTGPEAETLERIFSGRTPEKYWDGPFIMPVAGNVRTTSQFA
                     TRRCYNCAPGSKPTSYHGGMDMAVNEGTPVRAPAAGRVVFAGKLQVRGNTVIIDHGLG
                     VFTLLAHNSRLLVQEGQMVQQGERVSLSGNTGLSNGPHLHGDVHASGPPVEPVEWVKR
                     QMP"
     gene            2156..2755
                     /locus_tag="VFH60_12280"
     CDS             2156..2755
                     /locus_tag="VFH60_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET6314603.1"
                     /translation="MEQELLELLSAREGHFRLESGYHGGLWLDLDPLFVIPGRIQPFV
                     ETFAERLRGHAVAAVCGPLVGGAFLAQAVASLLGVEFYYAERFVPPVRDTATLYPVEY
                     RVPGGVGRMLRGKAVAVVDDAVSAGSAVRGTLATLEAYGAKPVVVGALLVLGGQAEQF
                     CRERGLALEYVARLPYQVWLPGECPLCASGLPYEDTGAP"
     gene            2788..3774
                     /gene="fmt"
                     /locus_tag="VFH60_12285"
     CDS             2788..3774
                     /gene="fmt"
                     /locus_tag="VFH60_12285"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET6314604.1"
                     /translation="MSEAKGTKSESPNPSLVFMGTPQFAVPSLRALAGEPYDITVVTQ
                     PDKPAGRGGRVTPPPVKVLAQELGLPVLQPASLKDPGFRALLAEMQPDVTVLVAYGEY
                     VAPVLLDLPRHGSINLHPSLLPRWRGSTPIQSAIMAGDEVTGVSIIRMDRGLDTGPIL
                     AQRSTPLAPDESHPELSARLANMGAELLAETLPRWLKGEIEPVPQTEEGATLTRTLSK
                     EDGLIDWSAPAEEIDRRVRAFQPWPGTYTYWQGRLLKVLRARPVPAEGDRVSGTVLAL
                     QQPGSPRSLAVATGGGGALELLEVQLAGKPPVEARALLTGYPQMVGSVLSDE"
     gene            3939..4529
                     /locus_tag="VFH60_12290"
     CDS             3939..4529
                     /locus_tag="VFH60_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314605.1"
                     /translation="MAIRTPEPRLPLEPEEYPLYQPPRRVGCSGITIVTLLAIGAFLF
                     LVWRVTPPMAKAITDFPKTLPIVGGLFVDDDEVTAVGTATFPTQTAQALASPAASVVT
                     TPAPPTPAPTNTPVPEYVQVAHTGGQGVRLRKEPRQNAEFEVVAREGTVFQIIGPDTP
                     DPNDPTQAWRNVVLPNDGRSGYVLKRYLVPHSGPNP"
     gene            4835..5311
                     /locus_tag="VFH60_12295"
     CDS             4835..5311
                     /locus_tag="VFH60_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314606.1"
                     /translation="MSKSRVEREIEEILSKYEDEKGEKSRGKTEDRQVVEFRANRGVP
                     PRPSRYSRSAPSFTMPNWKRLSSGQYMLMAFGAALLGVMVGPFVPFLATLLVILAVVL
                     FIVPILLYVQSGTTSGGWSPTTEEKRWRGQVINMHDRTRRDPNDPLDGIKRFFRRR"
     gene            complement(5492..6265)
                     /locus_tag="VFH60_12300"
     CDS             complement(5492..6265)
                     /locus_tag="VFH60_12300"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6314607.1"
                     /translation="MELVRLTTYDEIELAGHLYLPEGRAEWSPGLLVCHGFGSSKERH
                     AAFGQLAAQAGFAALILDLRGHGESGGQVDANIFNDIAAGLLFLQNRPEVNPMSIAIR
                     GSSMGGWLALHTAAHLKDVSPVVAYCPANEMMLMTLMEEVAMVQRGHTSPMITGPLPR
                     VNVNSMMQLLYRVDVQRAARRLNPRPLLLVHCEGDELVPAHVSQRIYDEAREPKTLWL
                     LPGCNHSFAQHDPATSTRLLDWLTAFGTQTGKLSPDVIS"
BASE COUNT         1176 a         2035 c         2128 g         1237 t
ORIGIN      
        1 gcgagtggac ctttttcgtt gcccgcgtaa ggcccgtcct tatgacgtga cagccaggcg
       61 gctctttggt ggccctgcga tagccctgcc accatcaccc tgtggctgaa gccacgggct
      121 tacaagaggc aaagtcccct acggggacta agcaacatca ggtagtctcc gtaggagact
      181 tcgtctctgg caagcccgcc aattcattgg cagggcgacg gcggcagggc gtcctggcag
      241 agtgttcttg cacctcgcac tcggtactcg gcactcggca ctcggtactc ggcactcggt
      301 actcggcact cggtactcgg cactcggtac tcggtactcg gtccttcctt tgtgctataa
      361 ttagagtccc tgcccccgat ggaccgcttt acatggacct ggagaatttc tcattgcaac
      421 ggtcagaacc tggctcttcc gacgtaaacc tgccccggcg tgacgcgcct aacgtaaacc
      481 gctctcgtgt gttgatcgtc ctatcggcgt tgtcgttggc gctggtggtg gttgccgtgc
      541 ttgctgcctg ggtgtggccg agcgtcgccg gcagggccag cgggagcacc cctgacgtcg
      601 ctggcaccgg cacctcgtcg gcggctgatg cggcttccgc gccgggggac gtggccctgg
      661 tccaggcgag cagcacgcgc gtaccgccta cccaggtgcc ccctacggca acgcccacgc
      721 tcacgcccta cacaggcgac ccggtagccg cccgccccgt gcccagcctc ggccccggca
      781 tgcgctactt caccgagacg ggccacctcg tgtccggcga tttctacagc ttctatcgca
      841 gcgtgcaaaa gagcgccgac ctgtttgggc ttcccctcac cgaagccttc gcccagaagc
      901 tgcccgacgg cggcgtctac atggtgcagt acttcgagcg cgcccgtctg gagttgcacc
      961 ccgacctgcc cgcatcgcaa cgggtgcaga ttggcgcgct tggggccgct atcctgcaag
     1021 ggcgcagctt cgaccgcgtg tccccggtgc aaaacagttc gacccgcgcc tattttcggg
     1081 agaccgggca cacgatacag ggcggcttct tcgagttctg gcttgccaat ggcggactgc
     1141 gcctgttcgg gctgccactc agcgaggaga tgcacgaggg gggcctcacc gtgcagtact
     1201 tcgagcgcgc ccgcttcgag tactacccgg ggttggtcgg cacgccctac gctatccagg
     1261 tgtcgcccgt tgggtacgcg gccctcaagc agtcaagctt caacatcccg atgggcacca
     1321 tgctacgctt caacccgccc gcgctggccg aggggcacac gagcatagtg gaggtagccg
     1381 cctcgcccgg cgtgagcgtc acggcgcagt acgagggccg cacgctctac ttcaagctgg
     1441 acgaagggcg cgaggtggcc tgggcggtgc tgggggcggt gcctttccag gacgtggggc
     1501 cgcgccaggt gacgttctac tttgagcacg gcgacggggg ccgccgcgtg gtcacccgga
     1561 cgcttgagat aacgccttac aggttcccca gcgagacggt gcgcttcgac cctgagaccg
     1621 cagccttgct cgacccggcg tacaccgggc cggaggcgga gacgctggag cgcatcttct
     1681 ccgggcgcac gccggagaag tactgggacg gcccgttcat catgccggtg gcaggcaacg
     1741 tccgcactac ttcgcagttc gccacgcgcc gctgctacaa ctgcgcccca ggctccaaac
     1801 ccaccagcta tcacggcggc atggacatgg cggtgaacga gggcacgccg gtgcgcgcgc
     1861 ccgccgcggg cagggtggtg ttcgcgggca agctacaggt gcggggcaat acggtaatta
     1921 tcgaccacgg cctgggcgtg ttcaccctgc tggcccacaa ttcgcgcttg ctggtgcagg
     1981 aagggcagat ggtgcagcag ggcgagcggg tgtcgctgtc gggcaacacc ggcctctcca
     2041 acggccctca cctccacggg gacgtacacg ccagtggccc ccccgtcgag ccggtggagt
     2101 gggtgaagcg gcagatgccg tagctgattt tggattttgg actttggggt ggtatttgga
     2161 gcaggaacta cttgagctgc tgagcgcgag agaagggcat ttccggctag agtcggggta
     2221 tcatggcggg ctgtggctcg acctggaccc gttgtttgtt attcctgggc gtattcagcc
     2281 ttttgtcgag acgttcgcgg agcggttgag agggcacgct gtggcggcgg tgtgcgggcc
     2341 actggtgggt ggggcgtttc tcgcgcaggc ggtggcttct ctgctggggg tggagttcta
     2401 ctatgcggag aggttcgtgc ctcccgtgcg cgataccgct accctgtatc ctgtggagta
     2461 ccgcgttccg ggtggggtag gccgcatgct gcgcggcaag gctgtggctg tggtagacga
     2521 cgcggtaagc gccgggtcgg cggtgcgggg cacgcttgcc actcttgagg cttacggcgc
     2581 gaagccggta gtcgtaggcg cgttgctagt actgggcggg caggcggagc aattttgccg
     2641 ggagcgcggc ctcgcgttgg agtacgtcgc acggcttccc taccaggtgt ggctgccggg
     2701 ggaatgtccc ctctgcgcct ccgggctgcc atacgaagac acgggggcac cttgagccag
     2761 ccaggcaacc aaatccgcag cttgcccttg agcgaagcga agggtacgaa atccgaatcg
     2821 ccaaatccat ccctcgtttt catgggaacg ccgcagttcg cggtgccgtc tttgcgcgcg
     2881 ctcgccgggg agccgtacga catcacagtg gtcacgcagc cggacaagcc cgccgggagg
     2941 ggtggacgcg tgacgccacc gccggtgaag gtgctggcgc aggagcttgg cttgccggtg
     3001 ttgcagccgg cctcgctgaa ggaccccggt ttccgcgctc tgctggccga gatgcagccg
     3061 gacgtgacgg tgctggtggc gtacggtgag tacgtggccc ccgtgctgct ggacctgccg
     3121 aggcatggca gcatcaacct ccacccgtcg ctactgcctc gctggcgcgg gagcacgccc
     3181 atacagtcgg caatcatggc tggcgatgaa gtcacggggg tgagcatcat ccggatggac
     3241 cgggggctgg acacaggccc catcctggcg cagcggagca ccccgttggc acctgacgag
     3301 agccacccgg agctgagcgc gcgcctggcg aacatgggcg ctgagttgct ggccgagacg
     3361 ctacctcgct ggctgaaggg ggagattgag cctgtgccgc agacggagga gggggccacg
     3421 ctcacccgca cgctgagcaa ggaggacggc ctcatcgact ggtcggcccc cgccgaggag
     3481 atagaccgcc gcgtgcgagc cttccagccc tggcccggca cctataccta ctggcaaggt
     3541 cgcctgctca aagtgctccg cgcccgcccc gtgcccgccg agggagacag agtctccggc
     3601 actgttttgg cgctgcaaca gcccggctct ccgaggtcgc tggcggtggc aacaggcggt
     3661 gggggtgccc tcgaattgct ggaagtgcaa ctggcgggca agcccccggt agaggcacgc
     3721 gccttgctta ccggctaccc tcagatggtg ggtagtgtgt tgagtgacga gtgacgagtg
     3781 gcccttaatt cgtaatgccc ggcacgtttt gcgcgtaatc ctattgcatc gctgctgctt
     3841 atgttgcaag cggcggcttt acacgttaca ttataggtag atcgtaatta ctcagtcctc
     3901 atcactcata actcatcacc cgtcacggaa ggcattagat ggcaattcgc acacctgaac
     3961 cgaggcttcc actggagccg gaagagtacc cgctgtacca gccgcccagg cgcgtgggtt
     4021 gctccggcat cactatcgtg acgttgttgg cgataggggc cttcttattc ctggtgtggc
     4081 gcgtcacgcc gcctatggcc aaggctatta ccgacttccc caagactttg cctatagtcg
     4141 gcggcctgtt tgtcgatgac gacgaagtca ccgcagttgg taccgctacc ttcccgacac
     4201 agaccgccca ggcgctcgcc tccccggcag cctcggtcgt aaccactccc gcgccaccca
     4261 cccccgcccc gaccaacact cccgtgcccg agtacgtgca ggtggcgcac accggcgggc
     4321 agggggtccg cctgcgcaag gagccgaggc aaaacgccga gtttgaggtg gtcgccaggg
     4381 aaggtactgt gttccagata atcggccccg acacgcccga tccaaacgac cctacgcagg
     4441 cctggcgcaa cgtggtctta cccaatgacg gccggtcggg ctacgttctg aagcgctacc
     4501 tggtgccaca ctctggcccg aacccttagg gcgacgagcg ctaagagacg gaaacgcaaa
     4561 gacgcaaaga gcctgtcctg aacgcagccg aagggacgca aaggacgcgc aaagagaagt
     4621 aaggttggat gttaccggta gctgatggta tccagtcgtc catcgtccat cgtccatcgt
     4681 ccatcgtccc tttgacatgg tacaggggcg gatgtacaat cagatatgag gcgcacgttt
     4741 aatgttgtgt agccactgac caccgaccac taaccactag ccacttcccg cgacaactaa
     4801 caaccagcca ttagcccgag agcgcaggta agagatgagc aagagcaggg tcgaacgaga
     4861 gatagaggaa atacttagta aatacgagga tgagaagggc gagaagtcgc gtgggaagac
     4921 cgaggaccgg caggtggtgg agtttcgcgc caaccgtggc gtgccgccga ggccctcgcg
     4981 ctacagtcgg agcgccccca gcttcaccat gcccaactgg aagcggctaa gctcgggcca
     5041 gtacatgctg atggcatttg gtgcggcgct gctcggcgtg atggtcggcc cgttcgtgcc
     5101 gtttctcgcg acactgctcg tcatcctggc cgtcgtgctg tttatcgtgc cgatactcct
     5161 gtacgtccag agcggcacca caagcggtgg ttggtcgcct actaccgagg aaaaacgctg
     5221 gcgcggccag gtcatcaaca tgcacgaccg cacccgccgc gaccccaatg accccctgga
     5281 cggcatcaaa cgcttcttcc gccggcggta ggattattta gacgaaaacg caaagacgca
     5341 aaggacgcaa agaacgcgca aagaggatga tggtgttcta agcaagagga ccagggtacg
     5401 acatgatgta ctctggtcct atttgtaacc actaaccata ttcttctctt tgcgcgttct
     5461 ttgcgtcttt gcgtctttgc gttttcgtct cctacgatat cacgtctggg gagagtttgc
     5521 cggtctgggt gccgaaggct gtgagccagt cgaggaggcg ggtagaggtg gctgggtcgt
     5581 gctgggcgaa gctgtggttg cagccgggga ggagccagag ggtcttgggt tcgcgggcct
     5641 cgtcgtagat gcgctggctg acgtgggccg gcaccaactc gtcgccctcg cagtgcacca
     5701 gcagcaaagg ccgggggttg aggcggcggg cggcacgttg tacgtccacg cggtagagca
     5761 gctgcatcat cgaattgacg ttcacgcgcg gcagggggcc ggtaatcatg ggcgaagtgt
     5821 gcccgcgctg caccatcgcg acctcctcca tgagggtcat gagcatcatc tcgttggcgg
     5881 ggcagtaggc cacgacgggg gaaacgtcct tcaggtgggc ggcggtgtgc agggccagcc
     5941 accctcccat cgaggagcct cttatcgcga tgctcatcgg gttcacttcg gggcggtttt
     6001 gcaggaataa cagcccggcg gcgatatcgt tgaatatatt ggcgtccacc tgcccgccgc
     6061 tctcgccatg gcctcgcagg tcgaggatca gcgcggcaaa cccggcttgc gccgccaact
     6121 ggccgaacgc ggcgtgcctc tccttcgaag agccgaagcc gtggcacacc agcagcccag
     6181 gcgaccactc ggcccgcccc tccggcagat acaggtgccc cgccaactcg atctcatcgt
     6241 atgtagtgag cctgaccaac tccatttatg cgtgcccctc ttcgagttgt tagttgttag
     6301 tgattagtaa gtcatatgtc cgaattatat actacaatag cctggtgtgc tgagtcgtac
     6361 aatggtaccg tgtgccgcaa agactttcgc ctgctatccg cccgcgctaa ctgccagaag
     6421 ctatctcgat aatgagcatg tagggcatag gtagctgcgg ccgcctctgt cattctgagc
     6481 gcagcgaaga atctcgtcct tcagcacaca gtctcacctt tcttaaatgt cggggcagag
     6541 tctcaagaga aaagggcaac atcggggtcg aggacg
//
LOCUS       DASRWZ010000776         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1159137, whole genome shotgun sequence.
ACCESSION   DASRWZ010000776 DASRWZ010000000
VERSION     DASRWZ010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159137"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..462
                     /locus_tag="VFH60_12305"
     CDS             217..462
                     /locus_tag="VFH60_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007457327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314608.1"
                     /translation="MPHDNDSEAAIAQRAEATGARAIGARAVGASAFGIFAIGAFAIG
                     VFAIGRLVIRSMRVGKLQIGELEVDRLRVRKLEILEG"
     gene            464..1657
                     /gene="coaBC"
                     /locus_tag="VFH60_12310"
     CDS             464..1657
                     /gene="coaBC"
                     /locus_tag="VFH60_12310"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET6314609.1"
                     /translation="MLRDKRVVLGVSGGIAAYKVADLASKLAQAGALVDVIMTQGAQQ
                     FVTPLTFQALTKRPVRTDIYADWLGEDTGHVGLARNADILVVAPATAHTIARLALGLS
                     DDMLCAVYLSTQAPVLVIPAMESGMFLHPATQEHLETLRRRGATVMQPGEGHLASGAS
                     GVGRLPEVPEMLAAIKHTLGRDGPLAGKKVVVTAGGTQEPLDPVRFIGNGSSGRMGYA
                     IAEEALNAGAQVTLITGAASAQPPYGVELVRARTTREMEAAVREAVRDADVLVMAAVA
                     DFAPRHTSEQKIKKTDAGLTVELERNPDILAGLATMDLPNLLRMGFAAETEDLLDNAR
                     AKLQKKKLDLIIANDAVASIGTESSSITFVRKDGNAQQLPQLPKEASARAIIEEISRL
                     IQERS"
BASE COUNT          412 a          586 c          623 g          290 t
ORIGIN      
        1 tgggtaggcg ggagtctaca acgtacagga tgatagctgc tagaggaaca ggggtgcaga
       61 atgagggaga gtcaagagcg ggcggctgat ccttcgctgc gctcaggatg acagggggga
      121 gtgtggttct gtgcaacgaa acatatgcca aacccataca aatctctgcg taacctctgc
      181 gccctctgcg gtgaaaattt agtaggagaa acgacaatgc cacacgacaa cgacagcgaa
      241 gcggccatcg cacaaagggc cgaggccacg ggagcgcggg ccataggggc cagggcggtg
      301 ggcgcatccg cgttcggtat attcgcgatt ggggcgttcg ccataggtgt gttcgctatt
      361 gggcggcttg tcatccgcag catgcgcgtg ggcaagctgc aaataggcga gcttgaggtg
      421 gaccgactgc gggtgcgcaa gttggaaata ctggagggct gagttgctgc gggacaagcg
      481 tgtagtgcta ggagtgagcg ggggcatcgc cgcgtacaag gtggccgacc tggcgtccaa
      541 gctggcgcag gcgggcgcgc tcgtggacgt cattatgacg cagggcgcgc agcagttcgt
      601 cacgcccctt accttccagg ccctcaccaa acgccccgtg cgcaccgaca tctacgccga
      661 ctggctgggc gaggacaccg gccacgtggg gctggcccgc aacgccgaca tcctggtcgt
      721 cgcccccgcc accgcccaca ctatagcccg cctggccctc ggcctctccg acgacatgct
      781 gtgcgccgtc tacctctcca cccaggcccc ggtgctcgtc atcccggcga tggagagcgg
      841 catgttcctg caccccgcca cccaggagca cctggagacg ctgcgccgcc ggggggcgac
      901 cgtcatgcaa cccggcgaag ggcacctggc gtcgggcgcg tcgggtgtgg gcaggctgcc
      961 ggaggtgccg gagatgctcg ccgcgataaa gcacaccctg ggcagagatg ggccgctcgc
     1021 gggcaagaag gtggtggtca cggcgggggg cacccaggag ccgctcgacc cggtgcgctt
     1081 catcggcaac ggctcgtccg gcaggatggg ctacgcgata gccgaggaag ccctcaacgc
     1141 cggggcgcag gtgacgctca tcacgggcgc ggcttcggcc caaccgccct acggggtaga
     1201 gctggtgcga gcgaggacca cccgcgagat ggaggccgcc gtgcgcgagg ccgtacggga
     1261 cgccgacgtg ctggtcatgg cggcggtggc cgacttcgcg cccaggcaca cgtccgagca
     1321 gaagatcaaa aagacggacg ccgggctgac cgtggagctt gagcgcaacc ccgacatcct
     1381 ggcagggcta gctacgatgg acctgcccaa cctgttgcgc atgggcttcg ccgccgaaac
     1441 ggaggacctg ctcgacaacg cccgagccaa gctccaaaag aaaaagctcg acctcatcat
     1501 cgccaacgac gccgtagcca gcatcggcac cgagtcgagc agcatcacct tcgtacgcaa
     1561 agacggcaac gcccaacaac tcccacaatt gcccaaagaa gcaagcgccc gcgccatcat
     1621 cgaggaaata tcccgcctca tccaggaacg aagctagtag agacggaacc gccaagacgc
     1681 caagaacgcc aagagcgcgc caagattaat taacgaatgg tgctagtttg acaaaaatat
     1741 gaggtactcc gatcctgttg ttaccgcact ccaggagcaa ggagaacctc atgtctacac
     1801 aggattttat cacggaactg ttctgcaggg tagatgattc catgaaagag gtaccgaagc
     1861 atagccaagc cagcctctac ccctctgaag tggtgacgct tggattgttg t
//
LOCUS       DASRWZ010000777         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_542774, whole genome shotgun sequence.
ACCESSION   DASRWZ010000777 DASRWZ010000000
VERSION     DASRWZ010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542774"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1327)
                     /locus_tag="VFH60_12315"
     CDS             complement(347..1327)
                     /locus_tag="VFH60_12315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6314610.1"
                     /translation="MPISLIMTVRNEAASIARLLESVLAQTLQPDEIVIADGGSTDGT
                     QEVVSSYMDRLPLRLLVLPGANISEGRNAAIRNASHDIIAVTDAGVRLEAEWLEALAG
                     PLLDEGAGVDVVSGFFVPDAQSPFELAMGATVLPALEDIEPGAFLPSSRSVAFRKAAW
                     EAAGGYPEWLDYSEDLVFDMRMKALGYRFTFAPEARVHFRPRSNLRAFFLQYYRYARG
                     DGKADLWRKRHAIRYATYIVGPLAAGWAWRHRRGMIGKGLFALMGVAAAGYCRRPYMR
                     LLPVLSRLPLGSRLYALAMVPVIRLTGDVAKMLGYPAGVWWRVRRGKDLR"
     gene            complement(1363..>2126)
                     /locus_tag="VFH60_12320"
     CDS             complement(1363..>2126)
                     /locus_tag="VFH60_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314611.1"
                     /translation="QALGDYIVPAAPGRKIDLGMVGSLVGWFPLDCLPNSREEPFARV
                     TLAALEAAHFHEGALFVNTGHSGWGTYLNMRVAGCRIRLGMDGGWQMMRWLLDHASPT
                     YNWPEAIHPHSKGGSAGDGHHGWASAEWLMLVRAMLFDDSREGLLEITPCLPLEWVGA
                     PGRLAVERAPTVFGTLSYAIEWDAGGRNMRLEISPDWHTPPRFMHWYMPGRPGDAAHP
                     GERARVLIDGKEGALTRGHVRIRGRASVVEVVRKP"
BASE COUNT          389 a          723 c          639 g          375 t
ORIGIN      
        1 gctgtaggac gaaattcttc gctacgctca gtggagcatc tcacaaataa gtatcaagaa
       61 agaggagcat attccccttg tcatcctgag cgtagcgaag gatcaggtgg gtagggaaga
      121 agctgtattg gagtactcac gggggcttgc cgccagcctc acgcattgct acagagaccg
      181 actgccacct gatccttcgc tacgctcagg atgacaaggg ggtagatggt cctgttgtgg
      241 ttactttctt gcaagctgat ccgctcagaa tgataaaggg gaacttgctc ccgcgcatgc
      301 agcactttgc agacaggccg gagcaggtgc tcgcgtgcta accagcctac cggaggtctt
      361 tcccacgtcg tacccgccac cacacccccg ccgggtaccc cagcatcttc gccacatccc
      421 ccgtgagcct gataaccggc accatcgcga gcgcgtacag cctggacccc agcggcagcc
      481 tactcagcac aggcaagagg cgcatgtaag gtctgcggca gtacccggcg gccgcgactc
      541 ccatgagcgc gaacaagcct ttccctatca tgcctcgcct gtgtcgccac gcccagcccg
      601 ccgcaagtgg ccccacaatg taggtggcgt agcgtatggc gtgccgcttg cgccacaggt
      661 cggctttacc gtcgccacgc gcgtaacggt agtattgcag gaagaacgct cgcaggttcg
      721 agcgcgggcg gaagtggacg cgcgcctccg gcgcgaacgt gaagcggtag cccagtgcct
      781 tcatgcgcat gtcgaacacc aggtcttccg agtagtcgag ccattcgggg tagccccctg
      841 ccgcctccca cgccgctttg cgaaaggcca ccgagcgact cgacggcagg aacgcgcccg
      901 gctcgatgtc ttccaacgcg ggcagcacgg ttgcgcccat cgccaattcg aacggcgact
      961 gagcgtccgg cacgaagaag cccgacacta catctacccc cgccccttca tccagcagcg
     1021 gccccgccaa agcttccaac cactccgctt ccaggcgcac ccccgcgtcg gtgacggcta
     1081 tgatgtcgtg gcttgcgttg cgtatggccg cgttgcgccc ctccgatatg ttggcccccg
     1141 gcaggacgag caggcgcagc ggcaggcggt ccatgtacga cgaaacgacc tcctgcgtgc
     1201 cgtcggtcga gccaccatcc gcgatcacta tctcgtcggg ttgcaacgtc tgcgccagca
     1261 cgctttcgag caggcgagcg atgctggccg cttcgttgcg cacggtcatg attagtgaga
     1321 tgggcatagt ctgaaaggtc tagcctggtg tcgcgcgggc tgttagggtt tgcggactac
     1381 ctctactacg ctcgcccgcc cccttatgcg gacgtgtccc ctggtgagag cgccttcttt
     1441 gccgtcaatg agcacgcggg cgcgctcgcc gggatgtgcc gcatcgccag gcctgccggg
     1501 catgtaccag tgcatgaagc gaggtggagt gtgccagtcg ggcgagattt cgaggcgcat
     1561 gttgcgccct cccgcgtccc attctatagc atacgataaa gtgccaaaca cggtgggggc
     1621 gcgctccacc gcgagcctgc ccggtgctcc cacccattcc aacggcaggc acggcgttat
     1681 ctccagcagc ccctcgcggg agtcatcgaa tagcatggcc cgcacgagca tcagccactc
     1741 cgccgacgcc cagccgtggt gcccgtcgcc cgcgctgcct cctttcgagt gtgggtgtat
     1801 cgcttcgggc cagttgtacg tgggcgaagc gtggtccagc agccaccgca tcatttgcca
     1861 gccgccatcc atgcccaggc gaatcctgca ccctgccacc cgcatattca ggtaggtgcc
     1921 ccaacccgaa tgcccggtgt tcacaaagag cgcgccctcg tggaagtggg ccgcctccag
     1981 cgcggcgagc gtaacgcggg cgaacggctc ctcccggctg ttgggcagac agtcgagggg
     2041 gaaccaaccc acaagcgacc ccaccatccc caggtcgatt ttgcggcccg gcgcggcagg
     2101 cacgatgtag tcgcccagcg cctgcg
//
LOCUS       DASRWZ010000778         5318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_611674, whole genome shotgun sequence.
ACCESSION   DASRWZ010000778 DASRWZ010000000
VERSION     DASRWZ010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611674"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1671
                     /locus_tag="VFH60_12325"
     CDS             127..1671
                     /locus_tag="VFH60_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6314612.1"
                     /translation="MQTANGPAPSESPGADISASPAEQSDKRTTSDSDARPPVVQIER
                     VSKQFRGVAALRGVSLNIERGEIFGLLGPNGAGKSTLLKLLLGFLHSDSGAVKLFGST
                     DLTRAHARIGYLPEQPSYHGNFSGREYVQFQARLAGLSARDARTMAARALKRVGLEEA
                     ANRRIRTYSKGMKQRLGLAVAVYACGDTPPELLVLDEPASGLAPEGQVAVREVLLDCN
                     RLGSTILLCSHQLTEVERICSQVGILRGGRLVALTRLDDQSRAVIVAAPRPRALELST
                     FLIEYLRNLHPAVIVKGGLVEDERLYVSLPTGDDVPHAAAMKAAAIKAMVDARWDIIS
                     LHVENRDLESIYLQAVRPSRKDAATPDPAGGKSLEAAATAEAEPAEVAATEVAATEVA
                     AAEGEQPQPGIVDADGAGALVDAAHTIGTEADEPGQPEGVEVVPPIQPLPVRPVSPNG
                     SAAVSQDAVAPPVEISAGEVPATGMRSGPSTAPLPPLPASIASEADAGPQDSWVRDLR
                     REGEEH"
     gene            1675..2484
                     /locus_tag="VFH60_12330"
     CDS             1675..2484
                     /locus_tag="VFH60_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314613.1"
                     /translation="MEATAPQTLSTAQAQDAPRITPRANPRVFWKLLRFTLSDYFRTP
                     WVFLNVAILVGVHAVFFTYKSGQSHFFGIEYAAAMLQAALTCTVMFSRSNRAESYAIL
                     ARQVPRASYVGAVMLAAWLVAALAYALSALFDMVYFSEWVTGQPLFDWQTPTNYALGS
                     LPVLVGAAFAVCLVTLLSTFVSTSAVRLGTMTLIAVLVMSFDARNFPVEPMRPVLQNI
                     PPVLAPLAGALKFAVEQDDIALVSLAVLGGYTLLMLVFALVLSGTRELVLD"
     gene            complement(2703..3485)
                     /locus_tag="VFH60_12335"
     CDS             complement(2703..3485)
                     /locus_tag="VFH60_12335"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6314614.1"
                     /translation="MPSTEPFDANASAFSDYQRSPWGRLLHDVAAANVQRHLDGQPLR
                     VLDAGGGSGVDAILFAGAGHAVTIIEPSVELLGQARANAGAAGVNARMEFHQAEIADI
                     PRLFAVAQFDLVLCHNVLQYVDDMAAALGAICSALVPGGLLSAICVNRYSEAYREVLQ
                     HHDPGAALEKLDTPAIFSGVFKTEVRAYTAEEVSQALGELGCVLLAQYGVRCVYDYIP
                     NELKNDPTFLVELERLEHALSGRYPYYLLARFFQLIVRKAAD"
     gene            3707..4336
                     /locus_tag="VFH60_12340"
     CDS             3707..4336
                     /locus_tag="VFH60_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314615.1"
                     /translation="MSGKLSPKIILEGTRLTLKTEIAFALNEHSRIVGPRKYRYHSPL
                     ISGEWCAFTNFPWGRGLINFEPQEEGLAMETYATWVRLFELQRFYSWIVDRFHISTRA
                     YQLQTYGKDYEFHWLEERLHALGFHIVLCTRMPESFHQAREERLKVSGNPSQYDDLGK
                     FIAEQELMRRLAGESLLPVLELDVSDSDVPRAADRVADWLESTGGLYAK"
     gene            4351..5136
                     /locus_tag="VFH60_12345"
     CDS             4351..5136
                     /locus_tag="VFH60_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HET6314616.1"
                     /translation="MDPIPPSQWTEYADINAMLGVLLDETRQILGGKLVGLYLYGSLV
                     TGDFDRGSSDIDLLAVTSSDIDRQEFARLDRMHQGVINRYQQWENRLEMAYLSATALQ
                     TYRRRVSNIAIISPGEPFHVKEAGKDWLLNWYVVREQGIALFGPSPKTIIEPITREEF
                     IQTVKSQVREWREWVEHGHTRPWQAYAILTMCRALYAVSTGEQASKKQAALWAAQALP
                     QWSSLINSALAWRAAWRDQDVDHEATFPETLRFVHFVIDRITA"
BASE COUNT         1061 a         1712 c         1578 g          967 t
ORIGIN      
        1 cttgcaagat gctccactta ggatgacaga gggcgatgga gtcctgccat cctacttccg
       61 caacctggcg cgaaataccc tccaccgtct ttccttaatc ccgcgcttta ctctataatt
      121 ctcactatgc aaacagccaa cggtcccgcg ccctcggaaa gccccggcgc ggatatctcc
      181 gcctctcctg ccgagcagag cgacaagcgt actacaagtg atagtgacgc ccggccgccc
      241 gtcgtgcaga tagagagggt gagcaagcaa ttcagggggg tcgccgcgct gcgtggcgtg
      301 tcactcaaca tcgagcgcgg ggagatcttc ggtctgcttg ggccaaacgg cgcgggcaag
      361 tccaccctgc tcaaattgct gctcggcttt ctgcacagcg acagcggcgc ggtcaagctc
      421 ttcggctcca ccgacctcac ccgcgcccac gcccgcatcg gctacctgcc ggaacagccc
      481 agctatcacg gcaacttctc cgggcgcgag tacgtgcagt tccaggcccg cctggcaggt
      541 ctgagcgccc gcgacgccag aactatggcc gcccgcgccc tcaagcgagt ggggctagag
      601 gaggccgcca accggcgcat acgcacctac tcgaagggca tgaagcagcg gttgggcctg
      661 gccgtggcgg tgtacgcctg cggtgacacc ccgcccgaac tgctggtgct ggacgaaccg
      721 gcctcggggc tggcccccga agggcaggtg gccgtgcgcg aggtgctgct cgactgcaac
      781 cgcctcggtt ccaccatact gctgtgctcg caccaactga ccgaggtgga gcgcatctgc
      841 tcccaggtgg gtatcttgcg gggcggcagg ctggtggccc tcacccggct ggatgaccaa
      901 tcgcgggcag taatcgtggc cgccccccgc ccccgcgccc tcgaattatc gaccttcctt
      961 atagagtacc tgcgcaacct gcacccagcc gtgatcgtga agggtgggct ggtggaggac
     1021 gagcgcctct acgtgagcct gcccacgggg gatgacgtgc cccacgccgc cgctatgaaa
     1081 gccgccgcca tcaaggccat ggtcgatgcc cgctgggata tcatctccct gcacgtggag
     1141 aaccgcgacc tcgagagcat ctacctgcaa gccgtacgcc cgtcccgcaa agacgccgcg
     1201 acaccggacc cggcaggtgg caagagcctc gaggcagccg ccacggccga ggcggaaccc
     1261 gctgaagtcg cggccaccga agtcgcggcc accgaagtcg cggccgcgga gggcgaacaa
     1321 ccacaacccg gcattgtgga tgccgacggt gcgggcgcgt tggtagatgc cgcgcacacg
     1381 attggcacag aggcagacga gccggggcaa ccggaagggg tcgaggtcgt accaccaatt
     1441 cagccgctac cggtgcgccc ggtgtcgcct aacggttcgg ctgcggtttc acaggacgca
     1501 gttgcgccgc cggtagagat ctcggccgga gaagtgcccg ccacaggcat gaggagcggc
     1561 ccctctaccg cgccgcttcc gccgctgccc gcatctatag catcagaggc agacgccggg
     1621 ccgcaagact cctgggtgcg cgacttaaga cgagaaggcg aggagcatta gaccgtggaa
     1681 gccacagccc cgcagacgtt gagcaccgct caagcccagg acgccccaag aatcaccccg
     1741 cgcgccaacc cgcgagtgtt ctggaagctg ttacgcttca cgttgagcga ctacttccgc
     1801 acgccctggg tcttcctcaa cgtggctata ctggtagggg tgcacgccgt cttcttcacc
     1861 tacaagtcgg gccagagcca cttcttcgga attgagtacg ccgccgccat gctgcaagcc
     1921 gccctgacct gtaccgtgat gttctctcgc agcaaccggg ccgagtcata cgctatcctg
     1981 gcaaggcagg tgccccgcgc ctcctacgtc ggggccgtta tgctggcggc ctggctcgtg
     2041 gcggcgctgg cttacgccct atcggccctg ttcgacatgg tctacttcag cgagtgggtc
     2101 acgggccagc ccttgttcga ctggcagacg cccactaact acgcgctggg cagcctgccg
     2161 gtgctggtgg gcgcggcctt cgcggtgtgc ctggtgacgc tgctcagcac gttcgtctcg
     2221 acctccgcgg tgcgactggg gacgatgacg ctcatagcgg tgctggtaat gtcgttcgac
     2281 gcgcgcaact tcccagtgga gcccatgcgc cccgtcttgc agaacatccc ccccgtgctg
     2341 gcgcccctgg ccggtgcgct caagttcgcg gtcgagcagg acgacatcgc cctcgtgtcc
     2401 ctcgccgtgc tgggcggcta taccctgcta atgctggttt ttgccctggt gctttcgggc
     2461 acgcgcgagc ttgttctcga ctagggagca tctgctgtag gaaacgggcg aacaaaggtg
     2521 gcccctgtca ttctgagcgc ggcgaagaat ctaggatgcc gcccctgcta ctatgttgaa
     2581 cgccagcacc acaggacgtg tggttcaaca tcatccctat accaacttct ttagattctt
     2641 cgctgcgctc agaatgacag aggccccttt gggtgattgc attgagcagt ttcagccagg
     2701 ccctagtctg ctgccttccg cacgatgagc tgaaagaaac gcgccagaag gtaatacggg
     2761 tacctcccgc tcagggcatg ttccagcctt tccaactcaa ccaggaaggt cggatcgttc
     2821 tttagctcgt tggggatata gtcgtagacg caacgcacgc cgtattgggc gagcagcacg
     2881 cagcctagct cccccaatgc ctggctcact tcctctgcgg tgtatgccct cacctcggtc
     2941 ttgaacacac ctgagaagat agcgggcgta tcaagcttct ccagggccgc accgggatca
     3001 tggtgctgca agacctcgcg atacgcctcc gaatagcggt tgacgcagat ggcggaaagc
     3061 agtccgcccg gcacgagggc agagcaaatc gctcccaacg ctgccgccat gtcgtccacg
     3121 tattgcagga cgttgtggca cagcaccagg tcgaattgcg ctaccgcgaa cagcctggga
     3181 atgtccgcaa tctcagcctg gtggaactcc atgcgtgcgt ttacacccgc cgctccggca
     3241 ttggcacgtg cttgacccag gagttccacg gaaggctcaa ttattgtgac tgcgtgcccc
     3301 gcccctgcga acaagatcgc gtcaacccca ctcccgccgc ccgcatccag cacgcgcaaa
     3361 ggctgcccat cgaggtggcg ctgcacgttc gcggcggcga catcgtgcag caggcggccc
     3421 cacggagaac gctggtagtc gctgaaggcg gaggcattgg cgtcgaacgg ctcagtagat
     3481 ggcatagcac tctcctggtg tatccgggcg cttgcacaac gtggcgtaac catcgccacg
     3541 cggttctggc gaaactataa ccgcaacatc atgtctcagt atgaggcttg cgcgcaattt
     3601 ctggcgtaca ataagggtaa gcatacaccc cacaacggag cgaggccagt agtccggcct
     3661 tgcttcatat gcgagtaaca gtaaaggttt ggaggtagca accgacatgt ccggcaagct
     3721 aagccccaag atcatccttg agggcacgcg actgacgctc aagaccgaga tagcctttgc
     3781 cctcaacgaa cactcgcgca tagtcggccc gcgcaagtac cgctaccatt cgcccctcat
     3841 ctccggcgaa tggtgcgcct ttaccaactt cccgtggggc cggggcctca tcaacttcga
     3901 gccgcaagag gaagggctgg cgatggagac ttatgccacc tgggtgcgcc tcttcgagct
     3961 gcaacgcttt tactcctgga tcgtggaccg cttccacatt tctacgcggg cctaccagct
     4021 acaaacctac ggcaaagact acgagtttca ctggctggag gagcgtctgc acgcactcgg
     4081 cttccacatt gtgctgtgca cccgcatgcc cgaatccttc caccaggcca gggaggaacg
     4141 gctgaaagtc tccggcaatc cctcgcagta cgacgacctg ggcaagttca tcgccgagca
     4201 agagctaatg cgccgcctgg ccggagagtc gctgctaccc gtgctggaac tggacgtctc
     4261 cgatagcgac gtgccccgtg ccgccgaccg ggtagcagac tggctcgaat cgaccggggg
     4321 cctgtacgcg aagtagaggg agagcattca ttggacccta tcccaccctc gcaatggact
     4381 gaatacgccg acataaacgc gatgcttggt gtcttgctgg acgagacgcg gcagatttta
     4441 ggcggtaagc ttgtcggcct gtacctctat ggttccctcg tcacgggcga cttcgatagg
     4501 gggagcagcg acatcgacct gctggccgtc acgtcctcgg acatcgacag acaagaattt
     4561 gcgcggttgg accggatgca ccagggcgtt atcaaccgct accagcagtg ggaaaaccgc
     4621 ttagaaatgg catacctgtc cgcaactgct ctgcaaacgt acaggcggcg tgtcagcaac
     4681 atagccatca tcagccccgg cgagccattc cacgtgaaag aagcggggaa agactggctg
     4741 ttgaactggt atgtcgtgcg agagcagggc atcgcgctat ttggcccttc accaaagact
     4801 ataatcgagc caatcaccag ggaagaattc atccagacgg tgaaaagcca ggtaagagaa
     4861 tggcgagaat gggtagagca tgggcacaca cgcccctggc aagcctacgc aatcctgacg
     4921 atgtgccggg cattgtacgc tgtcagcacc ggcgagcaag cgtcgaagaa gcaagcagct
     4981 ttgtgggcgg cgcaagccct cccccaatgg tcttccttaa taaatagcgc cctcgcctgg
     5041 cgcgctgcct ggcgagacca ggacgtagac cacgaggcga ccttccccga aactctccgg
     5101 ttcgtccact tcgtcatcga ccggattacc gcgtagggac ctcaaaaacg cgggggttgc
     5161 gaacggtagc caaagcccct tctatgtcat ttcgaacgca gtgagaaatc tcgtcctcca
     5221 ccgggatgtc acccctttct tagggcatac tataactgtc tgcgtaaaga aaagtggagc
     5281 ttgggtgctg agggacgaga tttctcacta cgttcgaa
//
LOCUS       DASRWZ010000779         4384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1228161, whole genome shotgun sequence.
ACCESSION   DASRWZ010000779 DASRWZ010000000
VERSION     DASRWZ010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228161"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..1373)
                     /locus_tag="VFH60_12350"
     CDS             complement(258..1373)
                     /locus_tag="VFH60_12350"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HET6314617.1"
                     /translation="MLGTRGVPASYSGFETCVEELGSRLVERGHEVTVYCRTHHIKYE
                     GETYKGMRLVKLPTIANKYLDTLVHSFVSSVHALFQPYDICLYFIAGNSLVTWIPRLV
                     GKRTIINVDGLDWKREKWPELARKYIRFAERMATVLPNAFLTDSTVVQRYYQDTYGVK
                     PPYIAYGSDVRPLLPGDTLGKYGLEPGKYILFVGRLVPENCAHHLVEAYCMLPDKHGM
                     KCVIVGDAPYAEEYKQSLKAGACEGVVFTGYVFGDGYRELSSSAYCFVETSGVGGTHP
                     ALLEAMGFGNCVVAHDTPENLETIGPAGFSYDGREGAASLCRVLARLLQDPQVVEERR
                     ALSLAHVRANYSWDRITSRYIDLFRWVLRGKRRRNAR"
     gene            complement(1440..>4384)
                     /locus_tag="VFH60_12355"
     CDS             complement(1440..>4384)
                     /locus_tag="VFH60_12355"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HET6314618.1"
                     /translation="GVTTTLETPSLPRQRVAARGVLGRRAITPLLRWLVLLVALFGLL
                     AGVYSSATHWRSDGANAVSAPLVPGTRLGQTFVSRYPGLSGLEVRVNGQYLRGRDAGA
                     TLVMHLRAAPGDASDLATATLRVTGDVQESQYQRFAFPALPDSRDKTYYVELESPDAT
                     PDNAPALLWWIQFDPNLPTDTYAGGTAYLDGQPQQSDLAFGLAYNPSPLDAFRVLASH
                     ASTNLPSLPVLLFLALAIAVTLLATYLALRPESEVRNPKSQILRFSLPIALTVALANG
                     VLFSLLVPPWQGPDEHGHYAYAALLYRHGMNDDAVQALQWQEGGKDHEEALALKAAVV
                     ASMSEHDWTRRVSGHPTPGAPAFPAGGQDLYFEFMWQIRQPALYYWLCAASLHVANAL
                     GAGIDPFADPAAALRVMRLVSVLLNVAMVGLAWLAGVLLGGRRLPWLRLVLPLAVALL
                     PMHTFIASVASNDAISDLAVTTVFVALVALLKWPFGWRVVGLAALSVLLLGAGAFTKL
                     TALVAGGPLLGLALLVWVGLLLTRYLPQWRVARRLPGAARDGDRFAPSPYVVPGIVAT
                     GVLLLCLVGALLLFRPSPAGAAWQAGGGPSMRPARVQMAAHSGSHAMQVQPGLTIYQW
                     VELPWPHPAYTATLTFWAQLGEPPELEPGEVAVPVESRAELAEDRRGTLSRIGADTLG
                     RIEYSMPFTPTRGEWRQFTISNTGTNADRKVWVRFTGGSTGFYVDDVSLTATPILAAG
                     TSERERARMPQEVRLPLFNTSMETGSLAVSPLGARVLRGETQDIVDVLVNPQAFDKLA
                     IWQRYAYRQFRSFWGNFGWLSIPLPELGYAIMNALIVVVLAALLSYGVRRLGTWTPME
                     WLGMVTLLALVSAILVTFARMMGPLSTSGVHTDPQGRYLFGYTVPVMWLMFEGLNIAW
                     SWVAGVARRNVLRGMARPRAGLSASTPATALGAATLMPWGAWLWCVGVAFFAGYSLLV
                     LVVPYYYR"
BASE COUNT          839 a         1454 c         1406 g          685 t
ORIGIN      
        1 gaaagggttg ccagatgggt aggtcgtagc gcagcgccat tgccatttcg agcgacaatt
       61 gcacaatgac gacatctata aggaacaaca acgccatgta gttggcattg agccgcttga
      121 acatggagcc tgctacctcc cctgttcccc cctgtgaact accacctaac agtatatggc
      181 aagggtatgc ataaagcaaa cctgacgagg cagccccgca cgcctgttct gaccgttgtg
      241 tataacggca gcttgcctca acgtgcatta cgcctcctct tgccgcgcaa tacccaccgg
      301 aacaggtcga tgtacctgct cgtaatcctg tcccacgagt agttagcccg cacgtgggcc
      361 agcgacaggg cgcggcgctc ctccaccact tgcgggtcct gtaacagccg ggccagcacg
      421 cggcagaggc tcgcggcccc ctcccgccca tcgtagctga acccggcggg gccaatcgtc
      481 tccaggttct caggcgtgtc gtgggcgacc acgcagttgc cgaagcccat cgcttccaag
      541 agcgccgggt gcgtgccccc aacgcccgaa gtctccacga agcagtaggc gcttgagctt
      601 agctcgcggt agccgtcacc gaacacgtag cccgtgaaca ccacgccctc gcacgccccg
      661 gctttcagcg actgcttgta ttcctccgca tagggcgcat cccccacgat aacgcacttc
      721 atgccgtgct tgtcgggcag catgcagtag gcttccacca ggtgatgcgc gcagttttcg
      781 ggcaccaggc ggcccacgaa caggatgtac ttgccaggct caagcccgta cttgccgagc
      841 gtatcgcccg gcagcagggg acgcacgtcg gagccgtagg cgatgtaggg cggcttcact
      901 ccgtaggtgt cctggtagta ccgctgcacg accgtggagt cggtgaggaa ggcgttgggg
      961 agcactgtgg ccatgcgctc ggcaaagcgg atgtatttcc ttgcaagctc cggccacttc
     1021 tcgcgcttcc agtcgaggcc gtccacgttg atgatagtgc gcttgcccac caggcggggt
     1081 atccacgtca ccaggctgtt gcccgcgatg aagtagaggc agatatcgta aggctgaaag
     1141 agcgcgtgca cggacgagac aaaggagtgc accagcgtgt cgaggtactt gttcgcgatg
     1201 gtgggcagtt tgaccagccg catgcccttg taagtctcgc cctcgtactt gatgtggtgg
     1261 gtgcggcagt agacggtaac ttcgtgccct cgctccacca ggcgagagcc aagttcctcc
     1321 acgcacgtct caaacccact gtagctcgcc ggtaccccgc gcgtgcctaa catcgcgatc
     1381 ctcaatgctg cccaccttgt ttgcgttttt cgttacggga aggccagggc cttattcgct
     1441 caccggtaat agtacggcac cacgagcacc agcaggctgt acccggcaaa gaaggcgacg
     1501 cctacgcacc agagccacgc cccccacggc attaacgtag cagcccccaa cgcggtcgcg
     1561 ggagtggagg cactgagacc cgcgcgcggc ctcgccatac ccctgagaac gttccgacgc
     1621 gccaccccgg ccacccacga ccaggctatg ttcagcccct cgaacatcag ccacatcacg
     1681 ggcacggtgt agccgaacag gtagcgccct tgcgggtcgg tgtgcacgcc ggaggtgctt
     1741 agcggcccca tcatgcgggc gaacgtgacc aggatggctg ataccagcgc gagcagcgtc
     1801 accatgccta accactccat aggggtccag gtgccgaggc ggcgcacgcc gtaagagagg
     1861 agcgcggcca acaccaccac gatgagcgcg ttcattatcg catagccgag ttccggcagg
     1921 ggtattgaca gccagccgaa gttaccccag aaggagcgaa actggcggta agcgtaacgc
     1981 tgccagatcg caagcttgtc gaacgcctgt gggttcacca gcacgtccac tatgtcctgc
     2041 gtctcgcccc gcaagacccg cgccccaagt gggctgaccg cgagcgaacc tgtctccata
     2101 gaggtattga acagcggcag gcgcacctcc tggggcatac gggcgcgctc tcgctccgag
     2161 gtaccggcgg ccagtatcgg agtggcggtc agggagacgt cgtccacgta gaaccccgtg
     2221 ctgccccccg tgaaacgcac ccacacctta cggtcggcat tcgtgccggt attggagatg
     2281 gtgaactgcc tccactctcc tcgggtgggc gtgaacggca tgctgtactc aatgcggccc
     2341 agggtatccg cgccaattcg cgagagagtg ccccgccggt cctcggccag ttccgccctg
     2401 gactctaccg ggaccgctac ctctcccggt tcgagctcag gcggttcccc aagttgcgcc
     2461 cagaaggtga gggtggcggt gtacgccggg tggggccacg gcagttcgac ccactggtaa
     2521 atggtcagcc ccggttgcac ctgcatcgca tgcgagccgc tgtgggcggc catctgcacg
     2581 cgggcggggc gcatggacgg cccacccccc gcctgccagg ccgcacccgc cgggctgggc
     2641 cggaacagga gcaatgcccc caccaggcaa agcagcaaaa ctccggttgc cacaatgccg
     2701 gggaccacat atgggctagg agcgaatcta tccccgtctc gcgccgcccc cggcaaacgc
     2761 cgggcgaccc gccattgggg cagatagcgc gtcagcagca gccccaccca caccagcaac
     2821 gccagcccca gcagtggccc gcccgccacc agcgcggtca gcttggtgaa ggccccggca
     2881 cccagcagca acacggaaag cgccgccaga cctaccaccc gccagccgaa gggccacttc
     2941 agcagggcca ccagcgcaac gaacaccgtc gttaccgcca ggtcggagat ggcgtcattg
     3001 ctcgccacgg aggcgatgaa agtatgcatc ggcaggagcg ccacggcgag aggcagcacc
     3061 aggcgcagcc acggcaggcg ccggcccccc agcagcacgc ccgccagcca ggccagcccc
     3121 accattgcca cgttcagcag gacagagacc aggcgcatga cgcgcaaagc agccgccggg
     3181 tcggcaaaag ggtcgatgcc cgcacccagc gcgttggcga cgtgcaggct ggcggcgcaa
     3241 agccagtagt agagggcagg ttggcgtatc tgccacatga attcgaagta gaggtcctgc
     3301 ccgcccgccg ggaaggcagg cgcgcccggc gtggggtggc ccgacacccg ccgcgtccaa
     3361 tcatgctcgc tcatcgaggc aaccaccgcc gccttgaggg ccagtgcctc ctcgtggtcc
     3421 ttgccgccct cctgccactg caacgcctgc accgcatcgt cgttcatgcc gtgacggtag
     3481 agcaacgcgg cgtaggcata gtgcccgtgc tcgtccggcc cctgccaggg cggcaccagg
     3541 agcgagaaga gcacgccgtt agccagcgcc acggtcaggg cgataggcaa ggagaagcgg
     3601 aggatttggg atttcggatt tcggacttcg gattccggtc gtagggccag gtaggtcgcg
     3661 aggagtgtga ctgctatcgc cagtgcgagg aagaggagta cgggcaaaga gggcaggttc
     3721 gtgctggcgt ggctggcgag gactcggaag gcgtccaggg gcgaggggtt gtatgccagg
     3781 ccgaacgcga ggtccgactg ctggggttgg ccgtccaggt aggcggtgcc gcccgcgtag
     3841 gtatcggtgg ggaggttggg gtcgaactgt atccaccaga gtagcgcggg tgcgttgtcg
     3901 ggcgtggcgt cgggcgactc tagctccacg taataggtct tgtcgcggct gtcgggtagc
     3961 gcggggaaag cgaaacgctg gtactggctc tcctgaacgt cgcctgtcac gcggagagtg
     4021 gcagtggcga ggtcggaggc atcaccgggc gctgctcgca ggtgcatcac cagcgttgcc
     4081 ccggcgtccc gcccgcgcag gtactggccg ttgacccgca cctccaggcc cgagaggccg
     4141 gggtagcgcg acacgaacgt ctgccccagg cgcgtaccgg gcacgagcgg cgctgacacc
     4201 gcgttagctc cgtcgctacg ccaatgcgtg gccgacgaat agacaccggc gaggaggccg
     4261 aacaaggcca ccagcaacac gagccaacgc aggagcgggg tgatcgcccg ccgccctaga
     4321 acaccacgcg cggcaacacg ctgtcgcggg aggctgggag tctcaagtgt tgtggtcacg
     4381 cctc
//
LOCUS       DASRWZ010000780        10226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1021993, whole genome shotgun sequence.
ACCESSION   DASRWZ010000780 DASRWZ010000000
VERSION     DASRWZ010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021993"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(683..3913)
                     /locus_tag="VFH60_12360"
     CDS             complement(683..3913)
                     /locus_tag="VFH60_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314619.1"
                     /translation="MLIPLLAAFPLSSKAARDPVPPQAYTAHAQQGGKATGNTSPSSP
                     AAVPVRGVSGDLWADVVVGKPRFSENSPFSIVPNKLFGSHGTIVDRSSTPNKLYVYDS
                     GNNRILGLDLGGCLSRPTNPLDCSADLVIGQPSMSHGACNGDSGYQLWPVRAEASAST
                     LCLQEESVLSVAEGGTGSSMAVDALGNLYVNDFWNNRVLKYINPFLTDTVADEVWGQT
                     DFTGKTCNRGLGSPDASTLCFNWGNSNNWTAGVELDALGNLWVVDNGNNRVLRFPPGS
                     HQADLVIGQPNFTSRGEGTGLNQFSDPNAARVNSRGWLYVGDHKNGRVLVFEPPFTTG
                     MSGRVFGADIAWPGGIEFEPGVSGVWIYDEGRKVLERRAEDTGSVVETLGILHDGNLL
                     GSATGSYGIDSDGNKYIAIGEGNYRYDVLMFAPGATTSPTKQLFGNGLGAGNSAGNQV
                     TASGMLGVSGVEVADNQLIATDDGRVLFWNDPASLYSGKPADGALGGGVNAFDQRARG
                     CCVALKADKSHHLYVAFAADAQWLDRLEVYQLPLTNGANPVDTIQLPLPVLGGGTVSA
                     TANFQALWGIAPTADSKFLWLSHSSTNRVFRIRDPLTNPTVDVILGQADNNGVDCNRS
                     TSANSTALPNTLCRPGSLALDRLGNLWVSDHSLEVEGNMRLLEFDASLFPTDNQQAIY
                     GPPATRQFPYLATWQPAFNSANMMVVGYNGYYPGNPIPVANYPYYDSAQRGRFPGVYS
                     NPLAPERSRLPDDFLKDFGSMSFTAVFDEEDNLYIGDLNRGRVLVYKKPALEPSPTST
                     PVPSSPTSTPTNTAASTATRTPAATFPATSTRTSTATRTASAVASASSTVAATATACS
                     ITFTDVPQASTFEPYVECLVCRGILSGYNTQERCPETGAPCFRPGDSITRGQMAKIVS
                     NAAGFEEDHQEVTFTDVPAGHTFYVYIQRLASRNIVSGYGTAANCPTGAPCFLPEATV
                     SRGQMAKFVASAAGFEEAVPPAQQTFADVPSNDTFWLYIERLASRSVVGGYACGQSPP
                     PGSACSTTVETCDASQRPYYRPCGLVTRGQAAKFVSLAFFPACDVP"
     gene            complement(4098..4940)
                     /locus_tag="VFH60_12365"
     CDS             complement(4098..4940)
                     /locus_tag="VFH60_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6314620.1"
                     /translation="MRKEADDNPANPKSKIQNLKSGEAPCPHCKGVGWLRMDVRVGDP
                     RFGKLYPCVCRTNATEESNLEELYKLSNLEAFSSKVFANFDPEVQGAAEAYDAAADFA
                     TNPEGWLVLMGGYGSGKTHLAAAIANHVVQDHRMQVYFAVAPDLLHHLRAAFAPNSTT
                     TYDDRFEQIRSVYLLVVDDLGAEQSTPWAVEKLYQIFNHRYNLRLPTVVTSNSDLDTL
                     DPRIRSRLCDPDLCRHVFLPSGDYRMRKMDPRFVQGGRNQGGTGGPRPQAGQRGSGYR
                     RNGG"
     gene            complement(4969..5826)
                     /locus_tag="VFH60_12370"
     CDS             complement(4969..5826)
                     /locus_tag="VFH60_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET6314621.1"
                     /translation="MKPFIGFSSDRLKTVRVPEGFFEDVLPAITSMLEMKVTLYLFWR
                     LARHGAPDGHAAGVPRMVSLDEMEGDTALRGGLSPVKGPRPWVEALRDGLELAVARGT
                     LLQMWVREEPNDLSEGRAEAWFMLNTRDNRTWLEALGKGKIDVTTTPLVATNGHNGHN
                     GSNGQNGHNGHTEDKSKIQNLKSKIQIVVERPTIFALYEQNIGLLTPLLAEKLQDAEG
                     RYPLEWIEAAFEEAVTNNKRNWRYIERILERWAVEGMNSGKDRGPAERSLDPDKYTKG
                     KYAFLFRPD"
     gene            complement(6281..7639)
                     /gene="dnaB"
                     /locus_tag="VFH60_12375"
     CDS             complement(6281..7639)
                     /gene="dnaB"
                     /locus_tag="VFH60_12375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET6314622.1"
                     /translation="MALATQALIERPVPYDLPAEEAVLGSLLLDRDAIIKIAPFLRPE
                     DFYREAHGWIYAAVLDLYARREPPDPVTLSTELERTGRLQSIGGSSYLIGLVNRTPTS
                     VHIEYYAHIVERTSVLRRLISAGGEIAALGYSEEQEVSAVLDKAESMLFAVSQRTSNQ
                     AFVSIEQVLTEYYERIENIQHNPGSVAGVPTGFHDMDEVTGGLQPSDMIILAARPGTG
                     KTSLALGIAHNAAMKGKHVGVFSLEMGRDQLVQRLLAVETGVDSQRLRLGYLSDDDWV
                     LVSDAIGRLASMPIYIDDSAGLSVSELRAKARRLKAEVGVDMLIIDYIQLMQGTARKD
                     GNRVQEVGEISRNVKLMARELNVPVLACAQLSRAVESRTKHVPMLSDLRESGSLEQDA
                     DMVMFIHREEMYDEETEKRGIAEIHIAKHRNGPLAVIPLRFWHTTTKFTDLEVYRTDP
                     LS"
     gene            complement(7902..8534)
                     /gene="rplI"
                     /locus_tag="VFH60_12380"
     CDS             complement(7902..8534)
                     /gene="rplI"
                     /locus_tag="VFH60_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET6314623.1"
                     /translation="MKVILTQNVPGVGEAGAIKEVADGYARNYLIPKKLAAPATRSSL
                     KQAEAQAEAIVRKANKQRDSLQQTASALDGKTVRIRARVGSENRLYGSITPADVADAI
                     GKQLNVTVDRRKVELAEAIHRTGTYTATADFGSGIVAQFQVEVAPEAAGAFGASAPAQ
                     SGAGASGADTGADAGGAEAEAGAGTPEGGAGTGDAASEEVAEQEAEANPT"
     gene            complement(8742..>10226)
                     /locus_tag="VFH60_12385"
     CDS             complement(8742..>10226)
                     /locus_tag="VFH60_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314624.1"
                     /translation="GGLPQQGYPISNEFSEVSDLDGKAYRVQYFERAVFEYHPENQPP
                     YNVLLSQLGTFQYKKKYPNGAPNQQPNNEAGSQLFTQTGKRLGGKFLTYWQTHGGLAQ
                     QGYPISDQFVEVNDLDGKPYTVQYFERAVFELHPENTGTPYEVLLSQLGTFRYQEKYQ
                     SPTPGPTSLPATPLPGATATGTRPSSGTGPPARAGHDLVYHDSLKLVLLVNGDDEGQP
                     AGTPSKIWGWDGREWRVVTSGGPEARSLGGVAYDPGRNVLVMYGGSTQDRVFSDTWEW
                     DGRAWSKKDVTGPGLLHHTSMVYDAERKELVMYGGNLQATESTRSNITVPEDTWTWNG
                     QAWRKAATQGLGPRYHYAMTYDAVRKMALVFGGATRSGDRNDIWGWNGAAWSEYLPQG
                     PEPSARSAARMAFDERAGKTILFGGVAGRTTLDDTWEWDGTGWRRLDVPGARPTARSH
                     HAMAYDRNRGRIVLFSGINDINLPPQLTDTWEWDGSSWAKVAGP"
BASE COUNT         1828 a         3046 c         3249 g         2103 t
ORIGIN      
        1 caggttgaag agaaaggcct cgatgccctg gcgtaccatg aaaaagcggt acgctttatg
       61 gttgtagtta gagttagagt tagagttaga gtttgggttc acgctcacct gctttggttt
      121 gcggcacaac aaagtgcctg gctagtatag caggctcgtg taagggaggc gcgggccgcc
      181 agtcacggtg cgccaggggg ttacggcttg tgcagccggg gtcgcgggcg tgccacgtgg
      241 aggcgtcacg ggcacggtat gcctgagacg ggtggcagcc tggcgagttg ttatcgcgcg
      301 gcgagactga ggttgcgcaa ggccccttgg agacctgctt tcctggtcgg tgctagcgat
      361 gtagttgaca ttgtgcgggc ctcctttccg gctgaacgag atgcagccgc cgtgtacttc
      421 cgccgcatta tacgggtgcc cctgccccga cgcatggggc tgtagatgta ttgtgaatag
      481 acggaaccgc caaggaccac gccaagagta cgaaaacgca aagacgcaaa ggacgcaaag
      541 aacgcgcaaa gattaggatt gttgttctta gcaaagagga gcagggtacg acatcgcgta
      601 ccctgctcct ctttgcaccc accaaacctt aatctatctt ggcgtggtcc ttggcgtctt
      661 ggcgtttaga taagctgagc gactacggaa cgtcgcaggc agggaagaag gccaggctga
      721 cgaatttggc cgcctgccca cgggtcacaa ggccgcaggg ccggtagtag gggcgctgag
      781 aggcatcgca agtctctacc gtggtgctac aggcgctacc tgggggtgga ctttggccgc
      841 aggcatagcc gcccaccacg ctgcgcgaag caagccgctc tatgtagagc cagaacgtgt
      901 cattggaggg tacgtcggca aaggtctgtt gagcgggtgg aacggcctct tcgaacccgg
      961 cggcgctggc gacgaacttg gccatctgcc ccctgctaac tgtggcctcg ggcaagaagc
     1021 agggcgcgcc ggtggggcag ttggcggcgg tcccgtagcc cgacacgatg ttgcggctcg
     1081 ccaatcgctg tatgtagaca taaaaggtat ggcctgccgg tacgtcggta aaggtgacct
     1141 cctggtggtc ttcctcgaag cccgcggcgt tggagacaat cttcgccatc tggccgcgtg
     1201 ttattgaatc gccgggcctg aagcaaggcg cgccggtctc agggcaacgc tcttgagtgt
     1261 tgtagccgga gaggataccc cggcagacaa gacactccac gtaaggctca aaggtactcg
     1321 cctggggcac gtcggtaaat gtaatcgagc aagcggtggc ggtggccgca accgtagagg
     1381 aagccgaagc gaccgcgctg gccgttcgag tggctgtcga tgtgcgtgta ctcgtggctg
     1441 gaaaagtagc agccggggtg cgcgtagccg tcgaggcggc ggtattggtc ggtgtcgaag
     1501 tggggctgct cggaaccggt gttgaggtcg gtgacggctc aagcgcgggc ttcttgtaga
     1561 caagcaccct gcccctgttc aagtcaccta tatacaggtt gtcctcttca tcgaagaccg
     1621 ccgtgaagga catcgagccg aagtccttca aaaagtcatc gggcagccgc gaacgctcag
     1681 gcgcaagggg attgctgtag acgccgggga agcggcccct ctgagcagag tcgtagtagg
     1741 ggtaatttgc aacaggtatt ggattgcctg gatagtagcc gttataacct accaccatca
     1801 tgttggctga attgaaggca ggctgccagg tggccaggta cgggaactgc cttgtggctg
     1861 gagggccgta aatggcctgc tggttgtcgg ttgggaatag cgaggcgtcg aactccagca
     1921 gccgcatgtt tccctcgact tccagggagt ggtccgagac ccacaggttg cccagcctgt
     1981 cgagggctag cgaacccggc ctgcacaatg tattggggag cgccgtggag ttggcggacg
     2041 tgctgcggtt gcagtcgact ccgttattat ccgcctggcc cagtattaca tctacggtgg
     2101 ggttcgtaag cgggtctcta atacgaaaca ccctgttcgt ggaggagtgg ctcaaccaca
     2161 agaacttgct atcggcggtc ggtgcgatac cccacaacgc ctgaaagtta gccgtcgcgg
     2221 atacggtgcc gccgccaagc accggcagtg gaagctggat cgtgtctacc gggttggccc
     2281 cgttagtcag cggtagctga taaacttcga gccggtcgag ccactgggca tccgccgcga
     2341 aggcgacgta cagatggtgg ctcttatcag ccttcagggc aacgcagcag ccacgagcgc
     2401 gctgatcgaa ggcatttacc ccaccaccca acgcaccatc cgccggcttg ccgctataca
     2461 gggatgcggg gtcgttccag aagagtaccc taccgtcgtc cgtggcaatc agctggttgt
     2521 cggcaacttc cactccactt acaccgagca tgccgcttgc cgtgacctgg ttacccgcgg
     2581 agttgcccgc acccaggccg ttgccgaaaa gctgcttggt cggtgaggtg gtcgcgccag
     2641 gggcgaacat gagcacgtcg tacctgtagt tgccttcacc gatggcgatg tatttgttgc
     2701 cgtcggaatc tataccataa gagccggtag cagagcctag caagttgccg tcgtgcaata
     2761 tgcccaacgt ctcaacaacg ctcccggtgt cctccgccct ccgttcgagt accttgcggc
     2821 cttcgtcgta tatccatact ccagaaacgc caggctcaaa ctcgatgccc cccggccacg
     2881 caatgtctgc cccgaacact cgcccgctca tccccgtggt aaaaggcggc tcgaacacca
     2941 gcactcgccc gttcttgtgg tcgccgacgt agagccagcc gcggctgttc acccgcgcgg
     3001 cattggggtc gctgaactgg ttaagcccgg taccttcacc cctggaggtg aaattaggct
     3061 ggcctattac caggtcggcc tggtgcgagc cgggagggaa acgcagcacc ctgttgttcc
     3121 cattgtccac cacccagagg ttgcccagcg cgtccagctc gaccccggca gtccagttgt
     3181 tgctgttgcc ccaattgaag cagagagtgg aggcatcagg agaccccagt ccccggttgc
     3241 acgtcttccc ggtgaagtcc gtctgccccc atacctcgtc ggccactgtg tccgtgagga
     3301 agggattgat atatttgagc acacggttgt tccagaagtc gttgacgtac aggttaccca
     3361 gggcgtccac ggccatgctc gaaccggtgc cgccctccgc aacactcagg acggactctt
     3421 cttgcaggca gagagtggag gcgctggcct cggctcgcac gggccacaac tggtagccgg
     3481 aatcgccgtt gcacgcgccg tgtgacatgc tgggctggcc tattacgagg tccgccgagc
     3541 agtcgagggg gttggtcggg cgggagaggc aaccacccag gtccagcccc aggatgcggt
     3601 tgttccccga gtcgtacacg tacagcttgt tgggtgtaga agaacggtcc actatcgtac
     3661 cgtgagaccc gaacagcttg tttgggacga tgctaaaggg gctgttttcc gagaaccggg
     3721 gcttaccgac aaccacgtca gcccacaagt caccggacac accgcgaacc gggacggccg
     3781 ccggactcga gggactcgtg ttgccggtag ctttgccccc ctgttgagcg tgggcggtat
     3841 aagcctgcgg gggtaccggg tcgcgcgcgg ccttgctgct tagcggaaac gcggccagca
     3901 ggggaatcag caacaggagt atgaaaccaa aaagccggtg tcgtaaagaa ggcaaggacg
     3961 gcggcgcagg caacatatac ccttcttgtc ttgagacaat cagtgaggga atgcaagcaa
     4021 ggaccctagc aaaagctaca gtccttgcta attgtagcct acgtgaccac aaacgtcaaa
     4081 tattgatagt ctgcctgcta cccaccgttg cgccggtagc cgctgccgcg ctgccctgcc
     4141 tgggggcggg ggccgcctgt gccgccctgg ttgcggccac cttgcacgaa gcgggggtcc
     4201 atcttgcgca tgcgatagtc gccgctgggg aggaagacgt ggcggcacag gtcggggtcg
     4261 cacaggcgcg agcggatgcg cgggtcgagg gtgtcgaggt cggagttgga ggtgacgacg
     4321 gtgggcaggc gcaggttgta cctgtggttg aatatctggt acagcttctc caccgcccag
     4381 ggcgtggact gctcggcccc caggtcgtcc accacgagga ggtagacgga gcgtatctgc
     4441 tcgaagcggt cgtcataggt ggtggtgctg ttgggggcga aggccgcgcg caggtggtgc
     4501 agcaggtcag gggccaccgc gaaatagacc tgcatgcggt ggtcctgtac cacgtgattg
     4561 gcaatcgcgg ctgccaggtg ggtcttgccg gagccatagc cgcccatgag cacgagccag
     4621 ccctctgggt tggtggcgaa gtcggcggcg gcgtcgtatg cctcggctgc gccctgcact
     4681 tcagggtcga agttggcgaa caccttgctg gagaaggctt ccaggttgct gagcttgtaa
     4741 agctcctcca ggttgctttc ctcggtggcg ttggtgcggc acacgcaagg gtatagcttc
     4801 ccaaaccggg ggtcgcccac gcgcacgtcc atgcgcagcc agccgacgcc tttgcagtga
     4861 ggacaggggg cttcgcccga ttttagattt tggattttgg attttggatt ggcgggatta
     4921 tcatctgcct ctttccgcac tccgaaatcc gaaattccaa tttccaaatt agtccggtct
     4981 gaagaggaag gcgtacttgc ccttcgtgta tttatcggga tcgagagatc gttcagcagg
     5041 tcccctatct tttccactgt tcattccctc cacggcccac cgttcgagga tgcgctcgat
     5101 gtaccgccag ttgcgcttgt tgttggtgac ggcttcctcg aacgccgcct ctatccactc
     5161 cagcgggtag cgtccttcgg cgtcttgcag cttctcggcc agcagcggcg tgagcaggcc
     5221 gatgttctgt tcgtacagcg cgaaaatggt gggacgctcg acgacgattt ggattttgga
     5281 ttttaggttt tggattttgg atttgtcttc tgtatgtccg ttgtgaccat tttggccgtt
     5341 gctcccgtta tgcccgttgt gcccgttggt cgcgaccagg ggggtggtgg tcacgtctat
     5401 cttgcctttg ccgagggctt ccagccaggt gcggttgtcg cgagtgttga gcatgaacca
     5461 ggcttcggcg cggccctcgc tcaggtcgtt cggctcctcg cgcacccaca tctgcaagag
     5521 ggtgccccgc gccacggcaa gctcaagccc gtcgcgcaac gcctccaccc agggcctcgg
     5581 ccctttcacg ggcgagaggc ccccgcgcag ggcagtgtcg ccctccatct cgtccaggct
     5641 gaccatgcgg ggcactcccg ccgcatggcc gtcgggtgca ccgtgacggg cgagccgcca
     5701 gaaaaggtag agcgtcacct tcatttcgag catggaggtg atggcgggca gcacgtcctc
     5761 gaagaagccc tccggcacgc gcaccgtctt gagcctgtcg ctggagaacc cgatgaaagg
     5821 cttcacggct ggggtgtcga gttgtgagtt gttagttgtg agttgatagc acttctcatc
     5881 tttgcgtgtg gtgttactcc ttgctctggc tgtacgtgcc tcgtatagaa ccaagcctct
     5941 tcttcaggtg cccacccccg cctttgtcat cctgagcgta gcgaaggatc agccacccgt
     6001 tcttgatact tcctacctta gcacctgcgt ttctcatatc ggcatcatcc ccgctgttga
     6061 agactctggc ctacccacct gatccttcgc tacgctcagg atgacaaggg ggaatacagg
     6121 ctgacaggag cggctttgat cctaaaccgt ggctgcttgc ggtgcgtact ataccactcg
     6181 ccaggcccgc cagccgcgat ttcctccgca aaccctcatt atcttcgcgt aacctccgcg
     6241 tcctctgcgc ctctgcggtg aattgtcctc cagcacggcc ctagctcaac gggtcggtgc
     6301 ggtagacctc caggtcggtg aacttggtgg tggtgtgcca gaagcgcagg gggatgacgg
     6361 cgagggggcc gttgcggtgc ttggcgatgt ggatctccgc gatgccccgc ttctcggtct
     6421 cctcgtcgta catctcctcg cggtggatga acatcaccat gtccgcatcc tgctccaaac
     6481 tacccgattc gcggaggtcc gacagcatcg ggacgtgctt ggtgcggctc tccacggcgc
     6541 gggatagctg ggcgcaggcg agcaccggga cgtttagctc gcgggccatc agcttgacgt
     6601 tgcggctgat ttcgcccact tcctgcacgc ggttgccgtc cttgcgggcg gtgccctgca
     6661 ttagctgtat gtagtcgatg atgagcatat caacgcccac ctcggccttg agccgccgcg
     6721 ccttggcccg cagctcgctc accgagaggc ccgcgctgtc gtctatgtag atgggcatgc
     6781 tcgccaggcg gccaatcgcg tcggacacca gcacccagtc gtcgtcgctg aggtagccga
     6841 ggcgcaggcg ctggctgtcc acgcccgttt cgaccgcgag gagacgctgc acgagctggt
     6901 cgcggcccat ttcgagggag aacacgccga cgtgcttgcc cttcatggcg gcgttgtggg
     6961 cgatgccgag cgcgagggag gtcttacccg tacccggacg ggctgcgagg atgatcatgt
     7021 cgctgggctg gaggccgccg gtcacctcgt ccatgtcgtg gaagccggtg ggcacgcccg
     7081 cgacggagcc ggggttgtgc tggatgttct cgatgcgctc gtagtactcg gtgagcacct
     7141 gctctatcga cacgaacgcc tgattgctgg tgcgctggga caccgcgaag agcatcgact
     7201 cggccttgtc gagcaccgcg ctgacttcct gctcctccga gtagccgagc gccgctatct
     7261 cgccccccgc cgagatgagg cggcgcagca cggaagtacg ctccactata tgggcgtaat
     7321 actcgatgtg caccgaggtg ggcgtgcggt ttaccaggcc gatgaggtag ctagagccgc
     7381 cgatgctctg caagcggccc gtgcgctcaa gctccgtgga gagggtcacc gggtcgggcg
     7441 gctcgcggcg ggcgtacagg tcgagcacgg cggcgtaaat ccagccgtgg gcctcgcggt
     7501 aaaagtcctc cgggcggagg aagggggcga tcttgataat cgcgtcgcgg tcgaggagga
     7561 gggaacccag cacagcctct tcggccggca ggtcgtaagg cacgggtcgc tctataagcg
     7621 cctgggttgc taatgccata gctacctcac atcaggtgat gagtaacgag taacaagtaa
     7681 tgagtaatta gtgctgagta ctctctaact caaaatccaa ctaacaacac aatatcgaac
     7741 tgactcaacc ccgtttactc gtagctacac gtacaagtac ctgtgagtag cacggcatta
     7801 ccgggaaagg aacaaaccct tatgaaagtg ctgagttccg agtaccaagt accgagtggt
     7861 aagtgcttac tcggaactcg gtactcacga ctcggaactg cctacgtcgg gttggcttcg
     7921 gcttcttgct cggctacctc ttccgaggcg gcgtcgcctg tgcctgcgcc accttcgggg
     7981 gtgcccgcac ccgcttctgc ctctgcacca cccgcgtccg cgccggtatc agcaccgctc
     8041 gcacctgcgc ccgactgggc cggggcggag gcaccgaagg cacccgcggc ttccggcgcg
     8101 acctccacct ggaactgggc gacgatgccg ctgccgaagt ccgcggtggc cgtgtaggtg
     8161 ccggtgcggt ggatcgcctc ggcaagctcc accttgcggc ggtcaaccgt tacgttgagc
     8221 tgcttgccta tcgcgtcggc cacgtcagcc ggggtaatag agccgtacag gcggttctcg
     8281 gagcctactc gcgcccgtat cctgacggtc ttgccgtcga gggcggaagc ggtctgctgg
     8341 agcgagtcgc gctgcttgtt ggccttgcgg acaatcgctt cagcctgggc ctcggcctgc
     8401 ttgagcgaac tacgggtcgc gggtgcggcc agcttcttag ggatgaggta gttgcgagcg
     8461 taaccgtcgg ccacttcttt gatcgcgcct gcctcgccca cgccgggcac gttttgcgtc
     8521 agtataactt tcatccgggg aatgctccct ctactttctg agtcaagggg gaaatccttg
     8581 cctcgaatta taccacaggg gggcacccca cccggaattg cacccggcat acacccgctt
     8641 tgcacacgtt atccacggcc ccaggcacga ggaaggggct tatccggccc tggatgtgga
     8701 aaacgtggag gcgaaaggca cagccggtct attgctgccg gtcacggacc tgctaccttc
     8761 gcccacgagc taccgtccca ctcccaggta tccgtaagtt gcggcggtag gtttatgtcg
     8821 ttgatgccgc taaaaagcac tatgcggccc cggttgcggt cgtaggccat cgcgtggtgg
     8881 ctccgggcgg tcgggcgagc gccgggtacg tccagcctgc gccaacctgt gccgtcccac
     8941 tcccatgtgt catcgagggt cgtgcggccc gccacgccac cgaacagtat ggtcttgccc
     9001 gcccgctcat cgaaggccat cctggcagca gatcgggcgg acggttccgg accctggggt
     9061 agatattcgc tccatgcggc tccattccag ccccaaatgt cattcctatc gccggaccgg
     9121 gtggccccgc cgaataccag cgccatcttg cgcacggcgt cgtacgtcat ggcgtagtgg
     9181 taacgcggcc caagaccttg cgtggcggcc ttcctccacg cctgcccgtt ccaggtccag
     9241 gtgtcttccg ggacagttat gttgcttcga gtggactcgg ttgcctgcaa gttgccgccg
     9301 tacattacca gttccttgcg ttcggcgtca tataccatcg aggtgtggtg taggaggccc
     9361 ggtcctgtaa cgtccttttt cgaccaggcc ctcccgtccc actcccaggt atcgctgaag
     9421 acacggtcct gggtgctacc cccgtacatg acgagcacgt tgcgccccgg atcgtaggct
     9481 acaccgccca gggatcgcgc ttcaggcccg ccggaggtaa caacccgcca ttcgcgccca
     9541 tcccagcccc atatctttga tggggtacct gccggttggc cttcatcatc tccgttgacc
     9601 agcaggacca gcttgaggct atcgtggtag accaggtcgt gacctgccct ggcaggcggg
     9661 ccggtgccgc ttgagggtct tgtgcctgtg gcggtcgccc ctggcaaggg agtcgcgggc
     9721 aaggaggtag gcccaggcgt gggagattgg tacttctcct ggtagcggaa ggtgccaagc
     9781 tgcgatagca gcacctcgta aggagtgccg gtattctcag ggtgcaactc gaacacggct
     9841 cgctcgaagt actgtaccgt gtaaggcttg ccgtcgaggt cgttcacttc aacaaactgg
     9901 tcggagatag gatagccttg ctgtgcaagc ccgccgtgcg tctgccagta cgtgaggaac
     9961 ttgcccccca ggcgtttgcc ggtctgggtg aatagctgag agcctgcttc gttatttggc
    10021 tgctggttgg gcgcgccgtt ggggtacttt ttcttgtact ggaacgtgcc cagttgggag
    10081 agcagcacgt tgtagggtgg ctggttctca gggtggtact cgaacaccgc ccgctcgaag
    10141 tactgcaccc tgtacgcctt gccgtccagg tcggatactt cgctgaactc gttgctgata
    10201 gggtaaccct gttgcggcaa gccgcc
//
LOCUS       DASRWZ010000781         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1090748, whole genome shotgun sequence.
ACCESSION   DASRWZ010000781 DASRWZ010000000
VERSION     DASRWZ010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090748"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..>2403
                     /locus_tag="VFH60_12390"
     CDS             274..>2403
                     /locus_tag="VFH60_12390"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET6314625.1"
                     /translation="MSRCMRLAKGRGRYAICALLAVAALGLLLAERTAGAAPRSATLW
                     SAPRSGNAFSFFDQAKRLAAPIFGPNVRANQDTTVYSQHEPSLAVSRVHTNTVIVASK
                     DYRQGNVKRVWIDGSTDGGATWPVQVHMPNLPATDSESDPVVMARDDGRIYVSCLTTG
                     NNGIWITWTDDDGQTWHPSVPIIQNQGGLQDKEWFAIDNNPASPYYHRMYMMYVPDPG
                     FGSGVVEHHSTDGGLTWSGQQPIAGNDTEYTYPVIASDGTVYNFMMENWGFGQTGTVQ
                     MTKSTNGGVTWSAPADVATAFQPTSPIRQPDQFRFFAIISAAVDPINGNLYVAWTDNR
                     NFNTNGTDVVYVRSTNGGASWSAVTRLSHDPTGVVRDHITPMLTVGADGKLHAFWLDR
                     RLDPNNRLFDSWYSSSTDGGNTWEPDTRVSTLSQDLNVGLPCCAGNYPAGDYWGLDTA
                     LDTVYVAWNDTRSGDQDILVSKGLMNFSDPPTTTPTVTGTPPTATSTRTATGTPSPTN
                     TAIATSTPVCTISVSSDDVPKNIPLGGTVTSTLTINQGANIADLDLVLGLNSDPTSGG
                     FRVSLTSPAGTTRVLVDAACGPYHNIYLDDEASQSIMGQCPPLTRQRFRPYTPLTAFD
                     GENSAGTWTLTATDVVGSEGELFAWGLLITSSGLCPTGTPTSPASTANPTAQGSATAT
                     RTATASATPQPECEPSWQTVITPDVGVF"
BASE COUNT          489 a          799 c          710 g          405 t
ORIGIN      
        1 cgaccacaga tgccaaagta tacgtccggc gatgcgccat tgtcaacttg attctgttat
       61 aatcgaatac gttcaggctt gccgggcgtt cgtgacataa gtactcgtat gaagggtagg
      121 ggcatgagcg tagcgggggg ccatgcctcg ccctgcttgg gtcacatcta aagccaatat
      181 gccagcctca ccagggcgag gcatgcctct cccctacagg aacattcctt tacccaatcg
      241 cgtggcagcc atttgcaagg gaggagcggg tttatgtcca ggtgcatgcg cctcgcaaaa
      301 gggcgcggtc gttatgctat ctgcgcgctc ctggcggtgg cggctctagg gctgttgctg
      361 gccgagcgca cggcgggtgc cgcgccccgc tctgccactc tctggtcggc tccccgtagc
      421 ggcaacgcat tctccttctt cgaccaggca aagcgacttg ccgcgcctat attcgggccg
      481 aacgtgcgcg ccaaccagga tactaccgtc tactcgcagc acgagccgag cctggcggtg
      541 agccgcgtcc acaccaacac ggtgatagtg gcctcgaagg actaccggca gggcaacgtc
      601 aagcgcgtgt ggatagacgg ctcgacggac ggcggcgcga cctggcccgt gcaagtccac
      661 atgcccaacc tccccgccac cgacagcgag agcgaccccg tagtgatggc gcgtgacgac
      721 gggcgcatat acgtctcctg tctcacgacg ggcaacaacg gcatctggat tacctggacg
      781 gacgacgatg ggcagacctg gcacccctcc gtgcccataa tccagaacca gggcgggctg
      841 caagacaaag agtggttcgc catagacaac aaccccgcca gcccatatta ccaccgcatg
      901 tacatgatgt acgtgcccga ccccggcttc gggagcggcg tggtggagca ccattcgacc
      961 gacggtggcc tcacatggtc gggccagcag ccgatagcgg gcaacgacac cgagtatacc
     1021 tacccggtga tagcgagcga cggcacggtc tacaacttta tgatggagaa ctggggcttc
     1081 gggcagaccg gcaccgtgca gatgaccaag tccaccaacg gcggggtaac gtggtccgcg
     1141 cctgcggatg tggcgaccgc cttccagccg accagcccta tccgccagcc cgaccagttc
     1201 cgcttcttcg ccatcataag cgcggctgtt gaccccatca acggcaacct gtacgtagcg
     1261 tggaccgaca accgcaactt caacaccaac ggcacagacg tggtgtacgt gcggagcacc
     1321 aacggcgggg ccagttggag cgcggtcacc cgcctgagcc acgaccctac gggtgtggtg
     1381 cgcgaccaca tcacgcccat gctgacggtg ggtgcggacg gcaagctgca cgccttctgg
     1441 ctggaccgcc gcctcgaccc caacaatagg ctcttcgata gctggtatag ctcaagcacc
     1501 gacggcggca acacctggga gccggacacc cgcgtgagca cattatcgca ggacttgaac
     1561 gtggggctgc cctgctgcgc cggcaactac ccggcaggcg attactgggg actggatacc
     1621 gcgctcgata ccgtgtatgt cgcgtggaac gacacccgta gcggcgacca ggacatcctc
     1681 gtgtcgaagg ggctgatgaa cttctccgac ccgcccacaa ccacccctac ggtgaccggc
     1741 acgccgccca cagccaccag tacccgcacg gccaccggca cgccatcgcc tacgaatacg
     1801 gcgattgcca cctccacgcc cgtgtgtacg attagcgtat ccagcgacga cgtgccaaaa
     1861 aatatacctc tcggcggcac ggtaacatca accctgacca tcaaccaggg ggcgaatata
     1921 gctgatttag acctcgtgct gggtctaaat agcgacccta cttcgggcgg cttcagggtg
     1981 agcctgacct ctcccgcggg cacgacgcgg gtgctggtgg atgcggcctg cgggccttac
     2041 cacaacatct acctggacga tgaagcgtcg caatctatca tgggccagtg tccgcctctc
     2101 acccgccaac gcttccggcc ttatacgccg ctaacagctt tcgatggcga gaactccgcc
     2161 ggcacctgga cgttgacggc tacagatgta gtcgggagcg agggagagct tttcgcctgg
     2221 ggcctgctaa tcacaagcag cggcctttgc cctactggca cgcccacttc ccccgcgtct
     2281 accgcgaacc cgactgctca agggagcgct actgccacgc gcacggctac ggcatcagcc
     2341 acgccgcagc cggagtgtga gccgtcgtgg cagacagtga tcacacccga tgtgggagta
     2401 ttc
//
LOCUS       DASRWZ010000782         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_268537, whole genome shotgun sequence.
ACCESSION   DASRWZ010000782 DASRWZ010000000
VERSION     DASRWZ010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268537"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..955
                     /locus_tag="VFH60_12395"
     CDS             227..955
                     /locus_tag="VFH60_12395"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET6314626.1"
                     /translation="MKLLVSKRPLLKKLLRRAGKRWLAWKYRHFSPDEGATGYVKVAG
                     LTLLLDRTVFDPRLHFTSRFFAVYLRRGAVVPKRGRVLDMGTGSGLLAISAALAGAGH
                     VTAVDLNAAAVLSARLNVAQYGLGGKVEVEQGDMFEPVKGERFDLIVCNPPYLKGEPT
                     SEGTLAYMAGDDYQWLRRFARDAAGHLASGGRCLLVLADSTDLSVVLDIFRRSGWAVR
                     LMARRDLIVERLFIVELIVQGKGL"
     gene            1059..2438
                     /locus_tag="VFH60_12400"
     CDS             1059..2438
                     /locus_tag="VFH60_12400"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET6314627.1"
                     /translation="MDPKQANDSTFPHDFLWGAATAAYQIEGSPTADGKGESIWDRFC
                     RIPGVIANGDTSDVACDHYRLWREDLALMKELGLDAYRFSISWPRVLPEGRGRLNAAG
                     LDFYDRLTDALLEAGIVPFVTLYHWDLPQALQDRGGWTDRDVAPYFAAYATAVGERLG
                     DRVKHWITLNEPAVVAFTGHLWGEHAPGMRSLRMAFAAAHNTHRAHGMAVQALRAAAP
                     GANVGITLNLHPVHPAGDTNEDQEAARLADSFHNRWFLDPVFYGRYPDDISQLLGDSG
                     PGVEEGDMATAQQPLDFLGINYYTRSVVRSRDSSQWFKEGKLPFETIKPDGREYTEMG
                     WEVYPDGLRELLERVHRDYPLPTYYVTENGAAFHDTVSAEGTVRDGRRQAYLESHFEA
                     VARAIGAGVPVKGYFAWSLLDNFEWAYGYSRRFGLIYVDYRTQDRIWKDSAKWYRRWL
                     ASRAAPASR"
     gene            2449..2769
                     /locus_tag="VFH60_12405"
     CDS             2449..2769
                     /locus_tag="VFH60_12405"
                     /inference="COORDINATES: protein motif:HMM:NF016197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HET6314628.1"
                     /translation="MPDYETARLKIDAIEAEMQSQGMWRAEPLPLEAYDFKAAFAMDT
                     MPFVHWLQFIFIPRVREIIDSEGTFLASSQVGAQAVREFDGINEASELVSLLSDFDHS
                     IRSG"
     gene            complement(2753..3115)
                     /locus_tag="VFH60_12410"
     CDS             complement(2753..3115)
                     /locus_tag="VFH60_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314629.1"
                     /translation="MHAVLTRLKALPGEMDRVKALAADLTMPAYVENAARGAFILASC
                     HCDEVLVLVLYATQPEAEMIECGEALRFLRDNWQLVLAEPPTSEAFEVLAGMTGGAPG
                     KPLSGDIRAFLSDLTRSL"
     gene            complement(3369..3683)
                     /locus_tag="VFH60_12415"
     CDS             complement(3369..3683)
                     /locus_tag="VFH60_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HET6314630.1"
                     /translation="MSEVKVTKWQQAQPPTEAALHRLLREEGLSAYAWQNGPRDAYST
                     HSHSYDKVIYVVRGSITFGLPDREESASLAAGDRLDLPRGVRHNAQVGPDGVTCLEAH
                     RQ"
BASE COUNT          688 a         1129 c         1205 g          737 t
ORIGIN      
        1 gtacgcgtcc aaatggccct tcaattctat tggcgaccat gttctactgg agatggtgag
       61 gaagtagtct cgatgatggg tctacaagtg actgtcggag tgatctcggc gttgaacgga
      121 aagatttatg ttggcattta caaattgcgc gtgtggctaa ctgttgtacc tatcggtgta
      181 cttttgtgct cagccaaccc gtaaatcagg cacaactcat gacaaaatga agctcttggt
      241 aagtaagcgt cccctcctaa agaagctgct acgccgggca ggtaagcgtt ggcttgcctg
      301 gaaatatcgc catttctcgc cggacgaagg tgccacgggg tacgtgaagg tagccgggct
      361 gactttgctg ctggaccgga cggtgttcga cccacgattg cacttcacca gccgcttctt
      421 tgcggtgtac ctccggcggg gggctgtagt cccgaagcga ggccgggttc tggacatggg
      481 gacgggcagc ggcttgctgg cgataagcgc ggcgctggcg ggggcggggc acgtcaccgc
      541 ggtagacctg aacgcggcgg cggtgctctc ggccaggctg aacgttgcgc aatatggcct
      601 tgggggcaaa gtggaggtag agcagggcga catgttcgag ccggtaaagg gcgagcggtt
      661 cgaccttatc gtctgcaacc caccatactt gaaaggagaa ccaacctccg agggcacgct
      721 cgcttacatg gccggagacg actatcagtg gctccgccgc tttgcgcgcg acgctgccgg
      781 gcacctggca tctggtggaa ggtgtctgct cgtgctggcc gatagcacgg atctatcggt
      841 agtcctcgac atcttcaggc gtagcgggtg ggccgtccga cttatggccc ggcgtgacct
      901 gatagtcgag cggctgttca tagtggaact gattgtgcaa ggtaagggcc tatagttatc
      961 ttgcgccaga agtgctgccc ggcgactggc tgtaccggcc cgggtggtag ctttcctgat
     1021 gctcgaaccg aagccacatt tactccggag gtatattttt ggacccgaaa caagctaatg
     1081 actctacctt cccccacgat ttcttgtggg gagcggccac cgctgcctac cagatagagg
     1141 gcagccctac cgccgacggc aagggagaga gcatctggga ccgcttctgc cgaataccgg
     1201 gcgtcatcgc gaacggcgat accagcgacg tggcttgcga ccactacagg ctgtggcgcg
     1261 aggacttggc gctcatgaaa gaactcggcc tggacgccta ccgcttctcc atctcctggc
     1321 cgcgcgtgct tcctgagggc aggggcaggc tgaacgcggc cgggctggac ttctacgacc
     1381 gcctcaccga cgcgctgctt gaggcgggaa tcgtgccgtt tgtcacgctc taccactggg
     1441 acttgcccca ggcgttgcag gaccggggtg gctggaccga ccgggacgtg gcaccctact
     1501 tcgccgcgta cgcgaccgca gtgggggaga ggctggggga ccgggtaaag cactggataa
     1561 ccctcaacga gcctgccgtg gtggccttca cggggcacct ctggggcgag cacgcacccg
     1621 gcatgcgctc gttgcggatg gctttcgcgg cggcccacaa tacccacagg gcgcacggta
     1681 tggccgtgca ggcgctcagg gcggcggctc ccggcgcgaa cgtgggtata acgctcaatc
     1741 tccacccggt ccacccggcg ggcgacacca acgaggacca ggaggccgcg cggctcgcgg
     1801 acagcttcca caatcgctgg ttcctcgatc ccgtgttcta tgggcgctac ccggacgaca
     1861 tatcacagct tctgggagac tccggccccg gagtagagga gggcgacatg gcgaccgccc
     1921 agcagcctct cgacttcctc ggcatcaact attacacccg cagcgtggta aggagccggg
     1981 acagcagcca gtggttcaag gagggcaagc tgccgttcga gacaatcaag cccgacggtc
     2041 gagagtacac ggagatgggc tgggaggtct acccggatgg gctgcgggag ctactggagc
     2101 gcgtgcaccg agattatccg ctgcccactt actacgtcac cgagaacggc gcggcgttcc
     2161 acgacacagt gtcggctgaa ggtaccgtcc gcgacggacg gcggcaggct taccttgaaa
     2221 gtcacttcga ggcggtcgca cgagctatag gcgcgggggt gccggtgaaa gggtatttcg
     2281 cctggtccct gctggacaac ttcgagtggg cttacggtta ctcacgccgc ttcggcctca
     2341 tctacgtgga ctacaggacg caggaccgaa tctggaaaga tagcgcgaag tggtaccggc
     2401 ggtggctcgc ttcaagggcc gcgcccgctt caaggtaggg gaactgggtt gcctgattac
     2461 gaaaccgcgc ggctgaagat cgatgccata gaggccgaga tgcagagcca gggcatgtgg
     2521 cgcgccgagc ccctgccgct ggaggcctac gacttcaagg cggctttcgc gatggacact
     2581 atgccattcg tccactggct gcaattcatt ttcattccgc gcgtccgcga gatcattgac
     2641 agcgagggga ctttcctggc tagcagccag gttggggcgc aagcggtgcg cgagttcgat
     2701 ggtatcaacg aagcaagcga gcttgtgtct ctcctgagcg acttcgacca ctctataagg
     2761 agcgggtgag gtcggacagg aacgcccgta tatcaccact caggggcttg ccgggcgcgc
     2821 cacccgtcat gccggccagc acctcgaagg cctcggaggt gggcggttcg gccagcacca
     2881 gctgccagtt gtcgcggagg aagcgcaggg cttccccgca ttcgatcatc tcagcctccg
     2941 gctgggtggc gtagagcacg agcactagca cctcgtcgca gtggcagctg gcgaggataa
     3001 aggccccacg cgccgcgttc tccacgtagg cgggcatggt caggtctgcc gccagggcct
     3061 tcacacggtc catctcgccc ggtagcgcct ttagccttgt caaaaccgcg tgcatcaagg
     3121 acctccatcg tgagcattga tgctgctcac tctatcttcg atggctgacc attttccata
     3181 ccaccgggta gccactttgt gctcattgtc ttgggcttcc gcgaccatga tgatgcggct
     3241 ggagacaccg aagctgggaa acttacttgt acagagacac ccctggctca taaagaccag
     3301 gcgtgctcgt aacggctctt taccgcgtcc ctatccatgt acgccgccct tgtcgcggcc
     3361 ggaaatatct actggcggtg cgcttccagg caggtgacgc cgtcggggcc gacctgcgcg
     3421 ttgtgcctca cccctcgcgg caggtccagg cggtcaccgg cggccaggga cgcgctttct
     3481 tcccggtcgg gcagcccgaa ggtgatcgag ccgcgcacca cgtatattac tttatcataa
     3541 gaatggctgt gagtggaata ggcatctcgc ggcccgtttt gccaggcgta ggccgaaagc
     3601 ccctcctccc ggagcaggcg gtgtagcgcg gcctccgttg ggggctgcgc ctgctgccac
     3661 tttgtgacct ttacctcgct cacggtaatc cctcctcgtg ttgtggcatc tcgcctgcgt
     3721 cctctgcgtc ctctgcgtcc tctgcgtcct ctgcgtcct
//
LOCUS       DASRWZ010000783         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1364866, whole genome shotgun sequence.
ACCESSION   DASRWZ010000783 DASRWZ010000000
VERSION     DASRWZ010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364866"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VFH60_12420"
     CDS             <1..1060
                     /locus_tag="VFH60_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HET6314631.1"
                     /translation="EVEEVTAAPDLGVTLDLTTAYLPPGTQVEYYWTLTDPLGNTTET
                     PTQAFKLVDERYSWKSLTDAQGRVTVHWYEGGSGFGKTLLGVATGALDRLQNDIDAGL
                     TRPAEVWVYASQDELLDALPANIPEWVGGKAFPHLALVMASIADDNMAELETKRTIPH
                     ELSHLVLYQATRNPYNAPPAWLDEGLAVYNQEAHDPAEEEALRWAAEHGALLPLNSLS
                     GSFGADEESALLAYAQSHSAVEFLLTDSRYGPDKLARTVAAFREGVTYDEAFQAGLGA
                     TVDEIDQQWLASLPYEVVPDRETVAASALDVVVWIVASIALLLFSAGGLLTAILLVRR
                     RRPAHAGATHPHPPGANL"
     gene            1064..1531
                     /locus_tag="VFH60_12425"
     CDS             1064..1531
                     /locus_tag="VFH60_12425"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6314632.1"
                     /translation="MPTVQATANIEGSAQEVFDFLADYRNIPRVQPQFNAARLVGDRE
                     RCVGACVELVGRFRGVPMQVQNRIVTYSPPYRLASISEGTVLSRNVWELQPLEHNFNA
                     TRVTFTVDYKVGGALGGLFTGLAASLFNKEIQAMTDEALRNLQGFFAAGAGGR"
     gene            complement(1748..2017)
                     /locus_tag="VFH60_12430"
     CDS             complement(1748..2017)
                     /locus_tag="VFH60_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314633.1"
                     /translation="MGERLHRSIAVLGNLGALVEVEEHAGTTYICGYSCPLANVTTKH
                     REACKLLEAMLVELTGLPVKERCRESEEAACWFEVSMPPRPAQGA"
     gene            2123..2198
                     /locus_tag="VFH60_12435"
     tRNA            2123..2198
                     /locus_tag="VFH60_12435"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2156..2158,aa:Gly,seq:ccc)
     gene            2398..>2809
                     /locus_tag="VFH60_12440"
     CDS             2398..>2809
                     /locus_tag="VFH60_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HET6314634.1"
                     /translation="MDRYDWGRLSHLQVGKYAEYFTKMEFTLYGFDVYTAEVDNKGID
                     FVVRKDHTRYYDVQVKSVRGRNYIFVPKKTWGNQLRENLLLAVVLFDAGESPKLYIIP
                     STRWHQPDELFVVREYEGKLSIPEWGLNVSRKNMP"
BASE COUNT          546 a          877 c          862 g          524 t
ORIGIN      
        1 cgaggtggag gaggtcactg ctgcccccga tttgggcgtg acgctcgacc tcaccaccgc
       61 ttacctgccg cccggcaccc aggtggagta ctactggacg ctcaccgacc cgctgggcaa
      121 caccaccgaa acgcccactc aggccttcaa gctggtagac gagcggtata gctggaagag
      181 cctcaccgac gcgcaggggc gggtgactgt gcactggtac gagggcggga gcggctttgg
      241 caagacgcta ctgggcgtgg cgacaggggc gctcgaccgc ctgcaaaacg acatcgacgc
      301 cgggctgacg cgccccgcgg aggtgtgggt ctacgcctcg caggacgaat tgctcgacgc
      361 actgcccgcc aacatcccgg agtgggtggg cggcaaggcg ttcccccacc tggcgctggt
      421 catggcctcg atagccgacg acaacatggc cgagcttgag accaagcgca ccatccccca
      481 cgagttgagc cacctggtgc tctaccaggc gacccgcaac ccctacaacg cgcccccggc
      541 ctggctggac gaggggctgg cggtctacaa ccaggaggcg cacgaccccg ccgaggaaga
      601 ggctttgcgg tgggccgccg agcacggggc gctgctgccc ctcaactcgc tgtccggctc
      661 gttcggcgcg gacgaggaat cggccctgct ggcctacgcc cagagccaca gcgcggtgga
      721 gttcctgctg accgacagcc gctacggccc cgacaagctg gcgcgcaccg tggccgcctt
      781 ccgagagggc gtcacctacg acgaggcgtt ccaggcgggg ctgggcgcga ccgtggacga
      841 gatcgaccaa cagtggctcg cctccctccc gtatgaagtc gtccccgaca gggaaacggt
      901 ggccgcctcg gccctggacg tggtcgtctg gatcgtggcc tccattgccc tgctgctctt
      961 cagcgcgggg ggcctgctga ccgcgattct actggtgcgc cgccgcaggc ccgcccacgc
     1021 gggggcaacc cacccccacc cgcctggcgc gaacctctaa gccatgccca cggtacaggc
     1081 caccgccaat atcgagggga gcgcgcagga ggtgttcgac ttcctggccg actaccgcaa
     1141 catcccccgc gtgcagccgc agttcaacgc cgcccggctg gtgggcgacc gcgagcggtg
     1201 cgtgggggcg tgcgttgagc ttgtggggcg gttccgcggg gtgcccatgc aggtgcagaa
     1261 ccgcatcgtc acctactcgc caccctaccg gctcgccagc atcagcgagg gcacggtcct
     1321 gagccgcaac gtttgggagc tacagcccct ggagcacaac ttcaacgcca cccgcgtgac
     1381 cttcacggtt gactacaagg tggggggcgc gttgggcggc ctgttcaccg gcctggccgc
     1441 cagcctgttc aacaaggaga tacaggcgat gaccgacgag gcgttgcgca acctacaggg
     1501 cttcttcgcg gcaggcgcag gtggcagata gcctcatgag gagtgcagga ttatagcagg
     1561 tcattccagg tcatcggaag tgccgcagac ttctcactat aaatcacctg cccccgatgt
     1621 agactccatc ttaagctccc tggacgaact ctgacaggag gatactcgct tgccagccgg
     1681 tttacggcgc tttactctca ggtagagcaa accgcctgga tatttcgccg ggagagaagg
     1741 tcagtcgcta ggccccctgc gcgggacggg gcggcatcga cacttcaaac cagcacgcgg
     1801 cttcttcgct ctcccggcac cgctccttga cgggcagccc ggtcaactcc acgagcatag
     1861 cctccagcag cttgcatgcc tcccggtgct ttgtagtcac attggcgagg gggcagctat
     1921 agccgcagat gtaggtggtg cccgcgtgct cttccacttc cacaagagcg cccaggttgc
     1981 cgagcacggc tatggagcgg tgcaggcgtt ctcccacatc gcctgctggt gcgcatccga
     2041 cgggggtcct ctacgcgtga tgggggaggt agctagcccc ttgaagtggg gccaggcatc
     2101 tgctataatt gagctaccga gcgcgggtgt aactcagttg gtagagtgtc tgcttcccaa
     2161 gcagaatgtc gtcggttcga gtccgatcac ccgctccata catctaaata ggcacgctta
     2221 gggttagggc ttgcgacgaa tggggtctga gtatattacg gtatatactc agacttcttg
     2281 cttttctgag gtactgtccg gtacacgtga ccgcacctag agcaaccatt ttcgtcaacc
     2341 ctattccact caattggcag atggtataag cctttatacc ctggccggag actgttaatg
     2401 gatagatacg attggggcag gctgagccat ctacaagttg gaaaatacgc cgagtacttc
     2461 acgaagatgg agtttaccct ctatggcttc gatgtctata cagctgaagt agataacaag
     2521 ggcatagatt ttgtggtccg taaggaccac acacgttact acgatgttca agtgaaatct
     2581 gtaagagggc gcaattacat ttttgtgcca aagaaaacct ggggcaacca gttgcgtgag
     2641 aatctgctcc ttgcggttgt tctctttgac gctggcgagt ctccgaaact ctacattata
     2701 ccttctaccc ggtggcacca gcccgatgaa ctcttcgttg tccgtgagta cgaaggcaag
     2761 ttgagcattc cagaatgggg attgaatgtc tccaggaaga acatgccaa
//
LOCUS       DASRWZ010000784         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1022411, whole genome shotgun sequence.
ACCESSION   DASRWZ010000784 DASRWZ010000000
VERSION     DASRWZ010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022411"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFH60_12445"
     CDS             <1..460
                     /locus_tag="VFH60_12445"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HET6314635.1"
                     /translation="GAKSVLDLPATLEWLETHGVPVLGFKTDYFPAFYTQATDPPLMV
                     DARVETAQEVADIFSMRKKLGLEGGVLVCVPIPSEVAMSNAELDGAISRALEDARRQG
                     VKGREVTPFLLGALSRSTEGRSLAANRALLENNAAIAAQISRALTPARYV"
     gene            473..1357
                     /locus_tag="VFH60_12450"
     CDS             473..1357
                     /locus_tag="VFH60_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET6314636.1"
                     /translation="MNILSLGDLLLDVVVRYDPLSGEADTGPDGVRMLPGGSAANFAV
                     HAARLGANVRFVSRVGRDWEGEMLARSLQSEGVAASVRTVQEAATGRVLVMVDAAGHR
                     RMWSYPGASGTLSPDDLDPEWFNNLDAFHLTGYSLLRDGPRAAALHALDLARSGGSPL
                     CTLDPNPPHLISDYDPMRYRDLLAQLRFDVIFPNLEEGRLLSGKETPGEIAASLLDIS
                     PGVVLTLGERGCIVATSTGTLEVDAAQVDRVVDVTGAGDAFAAGFVVEYLKSGDPRRA
                     AIAANMAAGEVVRRVGAR"
     gene            1426..1947
                     /locus_tag="VFH60_12455"
     CDS             1426..1947
                     /locus_tag="VFH60_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314637.1"
                     /translation="MAPISSSVEENQSAPPERGHARRPPLAEIDSWELWQAWKRGDKA
                     IVRPWIGLLVGFCVVLAAMYAFFYFSTSFSNDTIAQVGLLIVLVGFLYAIWRRSSPRR
                     GFALAVVALMAAVSGAAFLLWLLAYSLSNWNPWVAIIGNVIVGITGLLIGLPSLNAIA
                     RGQGSGIRDQELE"
     gene            1993..2895
                     /gene="ftcD"
                     /locus_tag="VFH60_12460"
     CDS             1993..2895
                     /gene="ftcD"
                     /locus_tag="VFH60_12460"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET6314638.1"
                     /translation="MRKLIECIPNFSEGRRQEVIDKVAAAVRAVTGVRLLDVSPNPDH
                     NRTVLTFVGEPEAVAGAAFACTAEAVRLIDMNKHKGEHPRMGAMDVVPFVPVAGVTMD
                     DAVALANSVGKRIADELEVPVYLYAAAATSPARKRLPDIRQGQYEGLKEAITRPERKP
                     DFGQPRMHPTAGATAVGARPPLIAFNVNLGTTNLQIAKNIAKGLRESSGGLVNVQAMG
                     VDLAAEEMVQVSMNLLDYTHTPIHRAYELVRLEAERYGVPVVASEIVGLVPMDALAGA
                     ADFYLRLKDFNRNQVLEARLLEED"
     gene            complement(2899..>3407)
                     /locus_tag="VFH60_12465"
     CDS             complement(2899..>3407)
                     /locus_tag="VFH60_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314639.1"
                     /translation="PLPGVLQGKRVVVVDDSIVRGTTTRPIVELLRRAGATEVHVRIH
                     SPAMSHPCYLGVDTARREELIAHRMSVPDIARHIGADSLGYLSLPGLFRAVGLGGERL
                     CSGCFTGNYPVPVQLDFAHADGKMALEDSGIRDQGPEANGHIQGGLELELDQVEMDRD
                     RVPVGFDL"
BASE COUNT          647 a         1022 c         1136 g          602 t
ORIGIN      
        1 tggggcaaag tcggtgctcg acctgccagc cacactggaa tggcttgaga cgcatggagt
       61 gcccgtgctc ggcttcaaga cggactactt cccggctttc tacacgcaag ccactgaccc
      121 gccccttatg gtggatgcca gggtcgagac agcgcaagag gtagcagata tattctccat
      181 gcgtaagaag ttgggcttgg agggaggcgt gcttgtatgc gtgcccatac cctcagaggt
      241 tgctatgagc aatgctgaat tggatggagc tatcagccgg gcgcttgagg atgcccgcag
      301 gcagggggta aaagggcgcg aagtgacccc cttcctgctg ggcgcgctat ccaggtccac
      361 agagggccgc tccctggcag ccaaccgcgc cctgttggag aacaacgcag cgatagccgc
      421 acagatcagc cgtgcgctca ccccggcgcg ctatgtgtaa cccgcgcagc ctgtgaacat
      481 cctctccctc ggcgacctcc tgctcgatgt ggtagtacgc tacgatcccc tctccggcga
      541 ggccgatacg gggcctgatg gagtgcgcat gctgcccggc gggtcggcgg ccaatttcgc
      601 ggtgcatgcg gcgcggctgg gggcaaacgt gcgcttcgtg agcagggtgg ggcgcgactg
      661 ggaaggggag atgctggcgc gctcgctcca atcagagggc gtagcagcat cggtgcggac
      721 cgtgcaggag gcagccacgg ggcgcgtgct ggtcatggta gacgctgccg ggcaccgccg
      781 catgtggtcg tatccgggcg cgagcggcac cctcagccct gacgacctgg accctgagtg
      841 gttcaacaac ctcgacgcct tccacctcac cgggtactct ctcttgcggg atggccccag
      901 ggcagccgcg ctccatgctc tcgatttggc gcgctccggc ggctctccac tctgcactct
      961 cgaccccaac cctccacacc tcatctcgga ctacgaccct atgcggtacc gcgacctgct
     1021 ggcccaactc cgctttgacg taattttccc taacctggaa gaaggcagat tgctgagcgg
     1081 caaagagact ccgggggaga tagccgccag cctgctggat atatctccgg gggtcgtact
     1141 gacgctagga gagcgcggct gcatcgtggc gacgagtacg ggcacgctcg aagtggacgc
     1201 tgcacaggta gacagagtgg ttgatgtgac aggcgcgggc gatgctttcg cggcgggctt
     1261 tgtggtagaa tacttgaaga gcggggaccc tcggcgggca gccatagctg ccaacatggc
     1321 ggcgggtgaa gtggtgcggc gggtgggggc acgataggtt caccgcagag gcgcggagac
     1381 gcagagaacc gtaatgtttt gccctatgca ggcataggct ggagagtggc cccaattagc
     1441 tcatctgtcg aagaaaatca gtccgccccg cctgagcgtg ggcatgcaag gcgacctcct
     1501 cttgccgaga tagactcgtg ggagttgtgg caggcgtgga agcgagggga taaggcgata
     1561 gtacggccct ggatcgggct gctggtgggc ttctgcgtcg tgctggcggc gatgtacgct
     1621 ttcttctact tctcaaccag cttcagcaat gacacgatag cgcaagtggg gttgctcata
     1681 gtcctcgtcg gcttcctcta cgcgatatgg cggcgcagct cccctcggcg cggcttcgcc
     1741 ctggcagtgg tggcgctcat ggcagcggta tcgggggcag ccttccttct atggctgctg
     1801 gcatacagcc tcagcaactg gaacccctgg gtcgccatca taggcaacgt gatagtcggt
     1861 atcaccggcc tgctgatcgg cctcccttct ctcaatgcga tagccagagg ccaggggtca
     1921 gggatcaggg atcaggaact agagtaactt acgcaatacg cagtacgcaa tacgtttcac
     1981 gggaagagga ccatgcgaaa gctaatcgaa tgtataccga acttcagcga gggtaggcgg
     2041 caggaagtta ttgacaaggt ggcggctgcg gtacgtgccg ttacgggcgt gcgcctgctg
     2101 gatgtgtcgc ccaatcctga ccacaaccgt accgtgctca ccttcgtggg cgagccagaa
     2161 gcggtggcgg gcgcggcgtt cgcctgcacg gcagaagcgg tcaggctcat agatatgaac
     2221 aagcacaagg gcgagcaccc acgcatgggc gctatggatg tggtgccatt cgtgcccgtt
     2281 gctggcgtga cgatggatga cgcggtggcg ctggctaaca gcgtcgggaa gcggatagcc
     2341 gacgagttgg aggtgccggt ctatctctac gcggcagccg ccaccagccc ggcgcgcaag
     2401 cgtctgcccg acatccgcca ggggcaatac gagggcttga aagaggcgat cacccggcct
     2461 gagcgcaagc ccgatttcgg tcagcccagg atgcacccca ccgcaggcgc gacggcggtc
     2521 ggcgcgcgcc cacccctgat cgccttcaac gtcaacctgg gcaccaccaa cctacagata
     2581 gccaaaaata tcgccaaggg cctgcgcgag agcagcggcg gcctggtgaa cgtgcaggcg
     2641 atgggggtgg acctggcagc ggaggagatg gtgcaggtct cgatgaacct gctcgattac
     2701 acgcacacgc ccatccacag ggcatacgaa ctggtgaggt tggaggcgga gaggtacggc
     2761 gtcccggtgg tcgccagcga gatagtgggc ctggtgccga tggatgccct ggcgggcgcg
     2821 gcggacttct acctgcgcct gaaggacttc aaccgcaacc aggtgctcga agcgcgactg
     2881 ctcgaagaag attagaggtt agaggtcaaa cccgacgggc acgcggtcgc ggtccatctc
     2941 cacctggtca agttccaact ccaacccgcc ctgaatatgg ccgttagctt ccggcccctg
     3001 atccctaatc cctgaatcct ccaacgccat cttaccgtcc gcgtgggcga agtctaactg
     3061 cacaggcacg gggtaattgc ccgtgaagca gcctgagcag agcctttcgc cgcccagccc
     3121 tactgcgcgg aagaggccgg gcaggctgag gtagccgagc gagtcggccc cgatgtggcg
     3181 ggctatgtcg ggcacgctca tgcggtgggc gattaactcc tcccggcggg cggtatccac
     3241 gcccaggtag caggggtggc tcatcgcggg cgagtggatg cggacgtgta cctcggtggc
     3301 cccggcacgg cgtagcagct ccacgatagg ccgtgtggtg gtgccgcgca cgatagagtc
     3361 gtccactacc accacgcgct tgccctgcaa tacccccgga agaggat
//
LOCUS       DASRWZ010000785         5075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_337206, whole genome shotgun sequence.
ACCESSION   DASRWZ010000785 DASRWZ010000000
VERSION     DASRWZ010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337206"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..598)
                     /locus_tag="VFH60_12470"
     CDS             complement(107..598)
                     /locus_tag="VFH60_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6314640.1"
                     /translation="MMPERVLMGGLLNTRRIRAVAICVFSRHGSILVAEGVDAVKGET
                     FYRPLGGTIEFSEHSSEAVVREIREELGAEVKPESLRFLGTLENIFVYNGLPGHEIML
                     VYDGELSDVTLYELGELHAHEDDGVPFRALWKPLEEFGRGAPLYPNGLLELLKGAKGS
                     SLS"
     gene            complement(650..1606)
                     /gene="deoC"
                     /locus_tag="VFH60_12475"
     CDS             complement(650..1606)
                     /gene="deoC"
                     /locus_tag="VFH60_12475"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET6314641.1"
                     /translation="MPHVERNPGTPLDLDWVYGTRINRSAVERRAATLVTRRTVKKEW
                     QAAWLLRAVTVIDLTTLAGDDTPGNVVRRCAKARRPVRDDLLEALGARGLGIRVGAVC
                     VYNNLVPVAVEALEGTNIPVAAVSTGFPAGQTSHESKLNEIRTSVAAGAREIDIVISR
                     AHVLTGNWQALYDEVRDFREACGDAHMKSIIATGELATMRNVYQASIVCMMAGSDFIK
                     TSTGKEATNATLPVSLVMARSIREYLDRTGHKVGFKPAGGIRTAKQALDWLILMKEEL
                     GNEWMMPHLFRIGASGLLTDIERQLEYFVTGRYSAAYRQPMP"
     gene            complement(2223..3581)
                     /locus_tag="VFH60_12480"
     CDS             complement(2223..3581)
                     /locus_tag="VFH60_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6314642.1"
                     /translation="MGAIRVLVNSWSNALKSRLSNNPMFRYPDLGLICLGVFIMLLGV
                     GAVVPVRAIYARDLGATAAELGLMASSFLLGTFMFQLPGGWASDKWGRKPLLVFGIAI
                     AGIVSFAFLLWDNPWYFIVLRFIEGAAGGAIQPASNAYVMDAVPAKERGAAFGWLGSA
                     FSAGFMLGPAAGGIMVDTMGYASPFIFGGVTSLFTAVFLLKRMHNRKPGDRPAEAEVP
                     SESASAGAETPVRKQIPRTLFRPALFSTLLLIAASGIEDGLFIALWTLWLDDLGASTS
                     YIGLTFVTFSLPLMVLMPITGKWADKYRLAPIIAIPAALASLVYLTYALTENLLLIAL
                     LGLFEGTLVAIRLPAISAFIANLSPDNARGRIQGITSTTRTVAGFVSSMVVIMLYEIN
                     HHFPFFMLFGTQLLVSVVGGILIWRIERRSIEANRMAAVAEATPVVRLEVASAGLDTA
                     AK"
     gene            complement(4031..4897)
                     /gene="nth"
                     /locus_tag="VFH60_12485"
     CDS             complement(4031..4897)
                     /gene="nth"
                     /locus_tag="VFH60_12485"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET6314643.1"
                     /translation="MPRIDTQQREKWPAPNEGPAMSEVEREVDLAREEAAVEPLRAKA
                     RAVLALLREEYGEPNWPMLDPLGTLIETLLSHRTADPQTWAAYNELKRRWPTWEELCD
                     APVEEIREAVGGTTWPEQKAPRVKAILQRIKDERGDFDLSFLYDMPLDEANAWLQSLG
                     GVGPKTAACVLLFACRRPVLPVDTHVHRVSIRLGLIGEKMEANEAHAVLPRFLPDPSN
                     APDVLAFHRNLLLHGQRICVWRDPKCPKCVVRDWCDYYATHPEKQAQAGELGQRKGKR
                     SAKEVLADTPEG"
BASE COUNT          924 a         1603 c         1587 g          961 t
ORIGIN      
        1 ttgctacaac aacaggaagg gtcaagaacg ggcggctgat ccttcgctac gctcaggatg
       61 acaaagggga gcggagtggg cctaatcgta atacctttac gccatcctac gacaaggaag
      121 agcctttagc gccctttagc aactctagca ggccatttgg gtagaggggc gcgccgcggc
      181 cgaactcctc caacggcttc cacagcgcac ggaacggtac cccatcgtcc tcatgggcgt
      241 gtagctcacc cagctcatat agcgtaacgt cggatagctc gccgtcgtag accagcataa
      301 tctcgtgtcc ggggagaccg ttgtagacga agatgttttc cagcgtaccg aggaagcgca
      361 ggctttcggg cttgacctcc gcgcctagct cctcccgtat ctcgcggacg acggcctcgc
      421 tgctgtgctc gctgaactcg atagtgccac caaggggacg gtagaaggtc tcccccttca
      481 cggcgtccac accttcagcg acgaggatgc tgccgtgtcg gctaaacacg caaatggcta
      541 cggcacgaat acgccgggtg ttgagcaggc cgcccattaa gaccctttcg ggcatcattg
      601 tctcacccaa tgcgctaacc ggtttcgtgc ctgtctcgga ctccgcgagc tagggcatag
      661 gttggcggta ggctgccgag tagcgcccgg tgacgaagta ctccagctgg cgctcgatgt
      721 cggtgagcag gccgctggcc ccgatgcgga acaggtgggg catcatccac tcgttgccca
      781 gctcttcttt cataaggatg agccagtcga gggcttgctt ggccgtgcgg atgcccccgg
      841 cgggcttgaa gccgaccttg tgcccggtgc ggtccaggta ctcgcgtatg gagcgcgcca
      901 tcaccaggct caccgggagg gtggcgttgg tagcttcctt gccggtggag gtcttgatga
      961 agtcggaccc cgccatcatg cacacgatgc tggcctggta tacgttgcgc atggtggcaa
     1021 gctcgcccgt ggcgatgatg gacttcatgt gggcgtcgcc gcaggcctct cggaagtcgc
     1081 gcacttcgtc gtagagggcc tgccagttgc cggtgagcac gtgggcgcgg gagatgacga
     1141 tatcgatctc ccgcgccccc gctgccaccg aggtcctgat ctcgttcagc ttgctttcgt
     1201 ggctcgtctg cccggcgggg aagcccgtag acactgcggc caccgggatg ttggtgcctt
     1261 ccagggcctc taccgctacc ggcacgaggt tgttgtacac gcacacagcg cccaccctga
     1321 tgccaaggcc cctcgcccca agcgcctcaa gcaggtcgtc acgcacgggg cggcgggctt
     1381 tggcgcaccg gcggaccacg ttgccggggg tgtcgtcgcc cgccagggtg gtcaggtcga
     1441 tcacggtgac ggcgcgaagc agccaggctg cctgccactc cttcttgacc gtgcggcggg
     1501 tgacgagggt ggccgccctg cgctccaccg cgctcctgtt tatgcgggtg ccgtacaccc
     1561 aatcgaggtc gaggggtgtg ccgggattgc gctcgacatg tggcatggtt tccactcgtt
     1621 gccgcgaggt gacggctgcg ctttgagtcg ccccgttctt cccgccgcgc ttgccgtcca
     1681 ggaccgtctg ctgctcgtct ttgaccgtgc tagccatcgt ctttccctcc gcaattgcgt
     1741 gccgtacatg taacgcttta gtatagcata acacgcgccg agggtagccg cgccgggtga
     1801 ggtttactgg aaattggacg gcaagaggtc acaatccata aatccaaacg ggacggcggc
     1861 ccgtagtatg aagtgaaagt tgccgctcta gtgtgttcgc cctccaccat tccgctagac
     1921 cggcaaccgc catttaaccc ctcactcggg acaactcggt aatggcagta tcgtagagag
     1981 acgaaaccgc caagacgcca agaacgccaa ggaccacgcc aagattaagt agggttttct
     2041 aagcgaaagg accagagtac atctaatgta ccctggtcct ctttgtactc tccaaaccct
     2101 tacttctctt tgcgcgttct ttgcgtcctt tgcgtctttg cgtttccgtt tcttggcgcg
     2161 ctcttggcgt ccttggcgtc ttggcggttc cgtctttact tgaagctatc gtcccactgc
     2221 tattacttgg ccgcggtgtc gagacctgcg ctcgcgacct cgaggcgaac taccggagtg
     2281 gcctcagcaa ctgccgccat ccggttggcc tcgatgctgc gacgctcgat gcgccagatg
     2341 agtatcccgc cgaccacgct caccagtagc tgcgtgccga agagcataaa gaacgggaag
     2401 tgatggttga tttcgtacag catgatgacc accatcgagc tgacgaagcc tgccaccgtg
     2461 cgcgtggtgc tggtaatgcc ctggatgcgg cccctggcgt tgtcgggcga taaattggcg
     2521 atgaaggcgc ttatcgcggg cagccgtata gccaccaggg tgccctcgaa caggccgagc
     2581 aacgcaatca ggagcaggtt ctcagtcagc gcataggtca ggtagaccag gctggcgagc
     2641 gccgccggga tcgcgatgat cggggcgagc cggtacttgt cggcccactt acccgtgatg
     2701 ggcatgagta ccattagcgg cagcgagaag gtaacaaacg tcagcccgat gtatgaggtt
     2761 gaagccccca ggtcgtccag ccaaagcgtc caaagcgcga tgaacaggcc gtcctcgatg
     2821 ccgcttgccg cgatcagcag cagcgtgctg aagagagcgg gccggaacag cgtacgaggt
     2881 atctgcttgc gcaccggggt ctctgcaccc gcacttgcgc tctcagatgg gacctcggct
     2941 tcggcgggcc ggtccccagg cttgcgattg tgcatccgct tgagcaggaa gacggcggtg
     3001 aagagagaag tcacgccacc gaagatgaag ggcgaggcat aacccatcgt atccaccatg
     3061 atgccacccg cggcaggacc gagcatgaag cccgcgctga aagccgatcc cagccaaccg
     3121 aaggccgcgc cgcgctcctt agcgggcacc gcgtccatta cgtacgcatt ggaggcgggc
     3181 tgtatggccc cgcccgccgc gccctcgatg aagcgcagca cgatgaagta ccagggattg
     3241 tcccacagga ggaaggcgaa cgacacaata cccgcgatgg cgataccgaa gaccagtagc
     3301 ggcttgcggc cccacttgtc ggacgcccac ccgccgggta gctggaacat gaaggtgccc
     3361 aggaggaacg aggaggccat caggccaagc tcggccgccg tggctccaag gtcgcgtgcg
     3421 tagatggcgc ggacgggcac gaccgcaccc actccaagaa gcatgatgaa gacgcccagg
     3481 cagataagcc ccaggtccgg gtagcggaac atggggttat tgctcagcct gcttttcagt
     3541 gcgttggacc agctattcac aagtactcta atggcaccca ttcccgtgtg cctccttccg
     3601 gcggcccctc ttagggccgt ccgcaaacaa cgatgagcag ccgccgagcc actcatcacc
     3661 gtaactatac cagattcggg aggcaccccg gcagagtcgc aagtcccatt ttgggaggag
     3721 gagagtaggg cttaagtccc actgttcggg gccgggtccg gtgcaaagta gggcgtagca
     3781 agcgcgtgtg cgtgggatgc gttgcggttg ccccgtgggg ccgccctgcg atagccctgt
     3841 ggctaaagcc acgggcttac cagagacgaa gtctccttcg gagactggta cacgtttcta
     3901 agtccccgta ggggactttg ccccttgtaa gcccgccaat tcattggcag ggcacaccca
     3961 tagggcgatg gtggcagggc gtccccacat ggcacccaca gggcatccca tgcacgcaca
     4021 acaggcccgc tcacccctct ggcgtgtctg cgagcacctc tttggccgag cgcttaccct
     4081 tgcgctgccc caactctcct gcctgggctt gcttctccgg gtgggtggcg tagtagtcgc
     4141 accagtcgcg gaccacgcac ttggggcact tggggtcgcg ccacacgcag atacgctggc
     4201 cgtggaggag caggttgcgg tggaaggcca gcacgtcggg ggcgttcgat gggtccggca
     4261 ggaaccgggg cagaacggca tgggcctcgt tggcctccat cttctcaccg atcaagccca
     4321 ggcgtatgct caccctgtgg acgtgcgtat cgacgggcag cacgggccgc cggcacgcga
     4381 acaggagcac gcaggcggcc gtcttcggcc ccaccccgcc caggctctgc aaccaggcgt
     4441 ttgcctcgtc cagcggcata tcgtagagga aggagaggtc gaagtcgccc cgctcgtcct
     4501 tgatgcgctg gaggatagcc ttcacacggg gagctttctg ctcgggccag gtggtgccgc
     4561 cgaccgcctc ccgtatctct tcgacgggcg cgtcgcacag ttcctcccag gtgggccagc
     4621 gccgcttcag ctcgttgtag gcggcccagg tctgcgggtc ggcggtgcgg tgggagagca
     4681 gcgtctcgat gagggtgccg agcgggtcga gcatgggcca gttaggctcg ccgtactcct
     4741 cgcgcaacag ggccagcacc gcccgcgcct tggcccgcaa cggctcgaca gccgcctcct
     4801 cccgagctaa atctacctcc cgctcgacct cgctcatcgc ggggccttcg ttcggggcgg
     4861 gccacttctc gcgctgctgg gtgtctatgc ggggcatggc ggctcctcct gtgtagtgcg
     4921 gagattaagt ccgcaactta tatgccatta ttggatgatg tggacacctc tagcgcccat
     4981 cgtccgctac acgcgggctg taatatcgtc tcaaccagct actcagcccg tccaaccccg
     5041 gaaacagcac ccgctcggtg atattcgcct ggtcc
//
LOCUS       DASRWZ010000786         4577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_200933, whole genome shotgun sequence.
ACCESSION   DASRWZ010000786 DASRWZ010000000
VERSION     DASRWZ010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200933"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2085
                     /locus_tag="VFH60_12490"
     CDS             <1..2085
                     /locus_tag="VFH60_12490"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HET6314644.1"
                     /translation="WRLTAQGINAQTQAGMASVPMTVTQPLLIRSVLPRFFLTGDNPH
                     PQAIVHNNTGQALDVEVSLVVSGAITLDSASPSAQRMTVQPGTQTIVNWSASVGKGDI
                     ANLRYWVHTVDNAGQASYREDAIGLSLPVKPFAAPEAVATSGEVAGTRADETIFLPYT
                     VNPLLGELVVQVSPSLAAATTNSLHFVEDYPYESTDMTVSRFLPLVVLEKVYNEQGLK
                     TPYSAQIPGILKSSLKRLGELQQPDGGWSWWEEGPSSWWHTGYVVQGLMEARNAGHAV
                     PDNMLQRGLERLRQFQQENAPQGVDDTYNLNMRAYTVYVLSRAGAASNDMKQEGRDLM
                     GQAPRISNHARAWLAMALGDMGMQAEAKTVLDSLAAAARQSSTTAHWEEERPDYWSMA
                     TNTRATALAVDALVTLSPNDPLTPKAVRWLMSAQKEGHWLSSMETSIGIISLAHYIRA
                     SKELTADYTWQVSAFDRLLGNAVANSSNVTQTVTMNLPVSAMPQNTLGTLGLARSSDQ
                     GKMYYQVSLRYYVPGEGIKSRSEGMAITRSYYKVGSDDPVKEVNAGDLVRVRLTIVAP
                     ETSYYVMVTDPLPAGLEGVNGSLNTTSFTERPPDLMGTIESAEGDDSGFGFDYGYYSR
                     WWRWGPFNNVEMRDDRTVLFASYMGPGTYVYEYYARATTPGVYMSLPAHTELLYYPDV
                     FGHSDGGQFTVK"
     gene            2224..3348
                     /locus_tag="VFH60_12495"
     CDS             2224..3348
                     /locus_tag="VFH60_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314645.1"
                     /translation="MVFLVLLLAGCGRYGQEVPGSPTPDATAEAASATLVDDFGPAAL
                     DYAFSSGLVATGTPQVLLSQKVSAAQLVCLGLGWAHWENHDQAVKYELVVYEGQLAGF
                     DTPGGLAPRIADSRFLLRVFDKGVMTAGGGPDWGRLRHTYGSSALPTDHPLYVSPEGI
                     PSLADLAPEEAAYPTAATVSNLVWGREGQELNVSQSQNGWTVSVARVYATTSWVILAF
                     TVSGPDRTYTVEAPGLTTPDGVSLTSNFAEQGGTFDGTYVGPNGFLVAFRAQNSPISP
                     STQSLRFEVPAIHVRSVFTPCNTLADTILPTPTRIPDNIMEILQNVTPQPTPPDLEVV
                     GPFTFDLQVPVVPSTPTPVLPTAIPTQPSELPPLPVPTGT"
     gene            4065..>4577
                     /locus_tag="VFH60_12500"
     CDS             4065..>4577
                     /locus_tag="VFH60_12500"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET6314646.1"
                     /translation="MSFNDRSRLDPSQVEDRRGRRGGAGLAIGGGGLGLVVVVVALLL
                     GVDPTKLGSPSVPPSSQVTDSAGSSNTLEEQCRTGADANARADCRIVGFVNSIQSFWT
                     DEFARHGERYSPATLVLFTDATEAACGYASAAAGPFYCLDDRKVYMDLGFFQELSTRF
                     GAKGGSFAEAY"
BASE COUNT          872 a         1459 c         1410 g          836 t
ORIGIN      
        1 tggcggctta ccgcgcaggg catcaacgcg cagacccagg cgggcatggc ctcggtgccg
       61 atgacggtta cgcagccgct acttatacgg tcggtgctgc cgcgcttctt cctcaccggc
      121 gacaaccccc acccgcaggc catcgtccac aacaacaccg gccaggcgct cgacgtggag
      181 gtgagcctgg tggtcagcgg ggctatcacg ctcgatagcg ccagcccctc ggcccagcgc
      241 atgacggtgc agcccggcac gcagacgatt gtcaactgga gcgccagcgt gggcaagggc
      301 gatatcgcca acctgcgata ctgggtgcac acggtggaca acgcgggcca ggcgagctac
      361 cgggaggacg cgattgggct gtccctgccc gtcaagccgt tcgccgcgcc cgaagccgtg
      421 gcgaccagcg gcgaggtagc gggcacccgc gccgacgaga cgatcttcct gccctacacg
      481 gtcaacccgc tgctgggcga gcttgtggtg caggtatcgc cttcgctggc ggcggccacc
      541 accaatagcc tgcatttcgt ggaggactac ccctacgaaa gcaccgacat gacggtgagc
      601 cgcttcctgc ccctcgtggt gctggagaag gtgtacaacg agcagggcct caagaccccc
      661 tactccgccc agataccggg catcctgaaa agcagcctca agcgattggg cgagcttcag
      721 cagccggacg gcggctggtc ctggtgggag gaaggcccct ctagctggtg gcatacgggc
      781 tacgtggtgc aggggctgat ggaggcgcgc aacgccggtc acgccgtgcc ggataacatg
      841 ctgcaacggg gcctcgagag attgcggcag ttccagcagg agaacgcacc ccagggtgtg
      901 gacgacacct acaacctgaa catgcgcgct tacacggtgt acgtgctctc ccgcgccggt
      961 gcggcctcca atgatatgaa gcaggagggc cgcgacctca tgggccaggc cccgcgcatc
     1021 agcaaccacg cgcgcgcatg gctggctatg gcgctgggcg acatgggcat gcaggccgag
     1081 gccaagacgg tgctcgacag cctggccgct gccgcccgac agtcgagcac caccgcccac
     1141 tgggaggagg agcgtcccga ctactggagc atggctacca acacccgtgc taccgccctc
     1201 gccgtggatg cgcttgtgac gctctcgccc aacgacccgc tcactcccaa ggcggtgcgc
     1261 tggctgatgt cggcccagaa ggaggggcac tggctgtcga gcatggagac ctctatcggc
     1321 atcatcagcc tcgcccacta catacgcgcc tcgaaggagc ttaccgccga ctacacctgg
     1381 caggtgagcg ccttcgaccg gctgctgggc aacgccgtcg ccaatagctc caacgtcacc
     1441 cagacggtga cgatgaacct gcccgtctcg gccatgccgc agaacacgct gggcacgctc
     1501 ggcctggccc gcagcagcga ccagggcaag atgtactacc aggtgagcct gcgctactac
     1561 gtgccgggcg aaggcatcaa gtcgcgcagc gagggcatgg cgatcacgcg cagctactat
     1621 aaggtaggca gcgacgaccc ggtgaaggaa gtcaacgcgg gcgacctcgt gcgcgtgcgc
     1681 ctgacgatag tggccccgga gacctcgtac tacgtgatgg tgaccgaccc gctgcccgcc
     1741 gggcttgagg gggtgaacgg ctcgctcaac accacctcgt tcaccgagcg cccgcccgac
     1801 ctgatgggca ccatcgagtc ggctgagggt gacgacagcg gcttcggttt cgactacggt
     1861 tactatagcc gctggtggcg gtgggggccg ttcaacaacg tggagatgcg cgacgaccgc
     1921 acggtgctct tcgccagcta catgggtccc ggcacctacg tctacgaata ctacgcccgc
     1981 gccaccaccc caggcgtcta catgtcgctc ccagctcaca ccgagctgct gtactacccc
     2041 gacgtgttcg ggcacagcga tggaggacag ttcactgtga agtagcattg taacgcgtaa
     2101 aacgtaaaac gtaatccgca cacctgcgag ttacgtttta cgttttacgc gtgatttcac
     2161 aaaggcagac agcatgtgaa gtcggaccat tcagcttacg cactcttttt acccgtacct
     2221 gctttggttt tccttgtctt gctgctggcc gggtgtggca ggtatgggca ggaagtacct
     2281 ggctcgccca cgccggacgc gaccgccgag gctgcttctg ccaccctggt ggacgacttt
     2341 ggaccggctg ccctcgacta cgccttcagt tccggactcg tggccacggg tacgccccag
     2401 gtgctgctga gccagaaggt aagcgcagcg caactcgttt gcctgggtct cggctgggcg
     2461 cattgggaga accacgacca ggcagtaaag tacgagcttg tcgtgtatga aggccagctg
     2521 gcgggtttcg ataccccggg cggcctggcg cctcgcatcg cggacagcag atttttactg
     2581 cgtgtgttcg ataagggagt gatgacggcg ggtggtggtc ccgactgggg acgtttacgc
     2641 cacacctacg gcagttcagc gctgcccaca gaccatcccc tgtacgtgtc acccgaaggt
     2701 atccccagtc tggcagacct cgcccctgag gaggctgctt acccaactgc ggcaaccgta
     2761 agcaacctgg tctgggggcg tgagggccag gagctaaacg tatctcagag ccagaacggc
     2821 tggacggtca gcgttgctcg cgtgtatgcc actacttcct gggtcatcct ggcctttaca
     2881 gtttcgggac cggaccgcac ttacacggtg gaagctcccg gcctcactac accggatggc
     2941 gtgtctctta catcgaactt tgcagagcag ggcggcacgt tcgacgggac ctatgtcggg
     3001 cctaatggat tcctggttgc gttccgcgcg cagaacagcc ccatcagccc gtcaacacag
     3061 tctttgcgct tcgaggtgcc cgcaatccac gtgcgctcgg tctttacacc ctgtaacacc
     3121 cttgcagaca cgatcttgcc tacgcctact cgtatcccag acaacataat ggagatattg
     3181 cagaacgtaa cccctcaacc cacaccaccc gacctggagg tggttggccc gttcaccttt
     3241 gacctgcaag tgccggtagt gccttccact ccgacgcctg tgctgccaac tgccataccg
     3301 actcagccat ccgagctgcc tcccctgccg gtccctacag ggacgtaagg gcatcgatac
     3361 ccctaaatac cggctgcgac aactgacatg ggccgggcat agacgaacac tcggtcccgg
     3421 cccatctgtt tggcagagta gagcgccccg tcgttggggt gtacctgatg ggctggctag
     3481 agtagggatg tagtacggtc cttccggcac aagacacgat agggtcttcc tgcaaaatgc
     3541 agcttatgcc gtaccaaagg tccgcccttg tcattctgag cgtagcgaag aatctcgtcc
     3601 tccagcaccc aaataccacc tttcttcacg ctggcagggt gccatgtgcc gctgcgagct
     3661 acggaaaagg ggaaaccggg gtgtaggacg agactcttcg ctacgctcag aatgacaagg
     3721 tccaccctcg ttcgcgtgct tcctgcactt tgcagatact ctctgttatt gccttacttc
     3781 gtgctgcctt ggccgccctg cgcaaatcct cgcgcggtct cgttattcat cagatggcat
     3841 agctattgca tgcgataagg agggcaattg aacgttgcgc cgggtagcgc taggtataat
     3901 tcctcacggc ttgccccaac cagcccgaac tgcggtgcgg ttcgaggctg tcgtgggacg
     3961 tggtcgccga acatagatac tcttaatcaa tgggtgaggt caggctgcat ccaggcagtc
     4021 aggggcgcac tgtcgaatcc atcgtgaggt agggggacag accaatgtca tttaacgatc
     4081 gatcgcgcct ggacccatcg caggtcgagg accggcgtgg ccggaggggc ggggccggcc
     4141 tggccattgg cggcggcggg cttggtctgg tggttgtagt agtggcgctg ctgctggggg
     4201 tcgatccgac caagttagga agcccgtcgg tgccaccgtc atcgcaagtg acggacagcg
     4261 ccggatcgag caataccctg gaggagcagt gccggacggg cgctgatgca aatgcccgtg
     4321 cggactgccg cattgtaggc ttcgtcaaca gcatccagtc gttctggacc gatgagtttg
     4381 cccgccacgg tgagcgctac agccccgcca cgctcgtcct cttcacagat gcaaccgaag
     4441 ccgcctgcgg ctacgccagc gcagcggcgg ggccgttcta ctgcctggac gaccggaaag
     4501 tctacatgga cctgggcttc ttccaggagc taagcactcg ctttggcgct aagggcggct
     4561 cgttcgctga agcctac
//
LOCUS       DASRWZ010000787         3885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_337331, whole genome shotgun sequence.
ACCESSION   DASRWZ010000787 DASRWZ010000000
VERSION     DASRWZ010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337331"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..2691
                     /locus_tag="VFH60_12505"
     CDS             262..2691
                     /locus_tag="VFH60_12505"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET6314647.1"
                     /translation="MSAPDLNIPAQGVADIESAAPHPNGTNGRHGGWRVSRAPLHLVA
                     GRQDGRHDGKQNGRRRPSRRKTAAAVPTIDTLLKTVSGYNPQANLAALRKAYDLAEMA
                     HEGQFRGTGEPYITHPLSVAVILAEMRLDTETLEAALLHDVIEDTPVTYEDIRTQFGP
                     TVAHMVEGVTKLGSIPKLRRAQDVAENAPKGERGEKAHREAQQAENLRKMFLAMFDDP
                     RVVLIKLADRLHNMRTLEGVAVEKQQRIAAETLEIYAPLANRLGMWQMKGELEDLSFA
                     YIKPDEYNSLVKMLRETKQARDRYVKRVIETLDSNLQEAGIKADIKGRVKHVYSLWQK
                     MERKRLPAGDIYDLLAVRIIVDTKQDCYAALGIIHSIWPPVPGEFDDYIARPKESTYQ
                     SLHTAVRSLDNKALEVQIRTHEMHEMAEFGIAAHWRYKEGKGGKRDPQYEDKIAWLRR
                     LMSVPEDGGSAQDFVDTLKSDIFQEHIYVFTPQGDIFELPRGATPIDFAFRIHSNLGY
                     QCAGARMNNKLVPLDTPLENGAVVSILKDKNRVGPSRDWLQSDRYLKTANARQKVRQY
                     FRRQRRDENVMQGLGILDQTLKHLGLLHVSHMDVLAYFPRYNSLEDFLEAIGNTDITP
                     QNITSKLGEFRQEELLQSTDALPVSVPTSVSLPSMPTTMSVSDLGGILTTLGRCCKPM
                     PGDEIIGYTTKLRGITVHRADCPNLQTVQDTGRLVRVSWGAKGLTKYPAGVRIQALDR
                     VGLLRDVTTVLADEKVNLISIHTNKTPSVATSTIIMTLDVTGVDQLYHVMGRLSTVRG
                     VYEVTRDTGQE"
     gene            2752..3732
                     /gene="lgt"
                     /locus_tag="VFH60_12510"
     CDS             2752..3732
                     /gene="lgt"
                     /locus_tag="VFH60_12510"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET6314648.1"
                     /translation="MPDRVAFTVFGIPVHWYGIMRMLGVLAASYVVYIEARRRGEDTD
                     HVWQLFPWALIAGILGARIGWVVSEAGNRRFNDFWEIFAIQQGGLSIQGTIIGGALAV
                     VIYCWRYKLRFFKWLDMIAPGVALGQAIGRWGNYFNQEAFGSPTNLPWGIPIDVRRQA
                     EVAGPNAGFAPSSDLRFHPTFAYEMIWNLFNFGLLMWLGRQRRIRLREGDIFWVYLIF
                     YSVGRYAIEELRVDSAIVSGFKAPQLFSIVAILFALVMIAVRHSPNSKVPLSATNLPH
                     GASVATHGRTRAAALDAGAESATPRVRRVARSSRRGRGATTTETSGAGTE"
BASE COUNT          819 a         1178 c         1235 g          653 t
ORIGIN      
        1 acttcccagc ccgtggcttt agccacaggg cgaccgtggc aggacatcgt tgacaacgtg
       61 aacgtggcag agcacaccca ggcacttaca gcgtgaatgg ctacacacag cctctcactt
      121 gaccctctgc cggtatggta gtatagttaa gtaggcacgc tgtcggtgca ttctacacag
      181 ggcgtgccat tttctacccc tttacactca atcccattgc gattcacacg ctagatatac
      241 aggctcaaag gcacagcagt tatgagcgcg ccggacctca atataccggc tcaaggggtt
      301 gctgacatcg agagcgcagc gccccacccc aacggcacca acggccggca cggcggttgg
      361 agggtttcgc gcgccccgct acacctggtc gcagggcgac aggacgggag gcacgacggc
      421 aagcaaaacg gccgccgtcg cccatcgcgc cggaagaccg ccgctgccgt gcctactata
      481 gacaccctgc tcaagacggt cagcggctac aacccccagg ccaacctggc cgcgctgagg
      541 aaggcctacg acctcgcgga gatggcccat gaagggcaat tccggggcac gggcgagcct
      601 tacatcacgc acccgctgag cgtcgccgtc atacttgccg agatgcgcct cgacaccgaa
      661 acgttagagg ccgccctcct gcacgacgtg atagaggaca cccccgttac ttacgaggat
      721 atacgcacgc agttcggccc aaccgtggcc cacatggtag agggcgtgac caagctcggc
      781 tcgataccca agctgcgccg agcgcaagat gtcgctgaga acgcccccaa gggtgagcgc
      841 ggcgaaaaag cccaccgaga ggctcagcag gccgagaacc tgcgcaagat gttcctcgcc
      901 atgttcgatg acccccgcgt ggtgctcatc aagctggccg accggctgca caacatgcgt
      961 acgctcgaag gcgtggcggt cgagaagcag cagcgcatcg ccgccgagac gctggagata
     1021 tacgcgcccc tcgccaaccg cctcggtatg tggcagatga agggcgagct ggaagacctc
     1081 tcgttcgcat acatcaagcc ggacgagtac aacagcctgg tcaagatgct cagggagacc
     1141 aagcaggccc gcgaccgcta cgtcaagcgc gtgattgaga cgctggacag caacctgcag
     1201 gaggccggta taaaagccga catcaagggg cgggtgaagc acgtctactc gctctggcag
     1261 aagatggagc gcaagcgcct gcccgccggg gatatttacg acctgctggc cgtgcgcatc
     1321 attgtggaca ccaagcagga ctgctatgcg gccctcggca taatacactc gatctggccc
     1381 ccggtcccag gcgagtttga cgactatatc gcgcggccca aggagtcgac ctaccagtcg
     1441 ctacatacgg ccgtgcggtc cctcgacaac aaggcgctcg aagtgcagat tcgcacgcac
     1501 gagatgcacg aaatggccga gttcggcatc gcggcgcact ggcggtacaa ggaaggcaag
     1561 gggggcaagc gcgacccaca gtacgaggac aagatcgcct ggctgcggcg gctgatgtcg
     1621 gtgcctgaag atggggggag cgcgcaggac ttcgtggaca cgctcaagag cgacatcttc
     1681 caggagcaca tctacgtatt cacgccgcag ggtgacattt tcgagctacc gaggggcgcg
     1741 acgccgatag acttcgcctt ccgcatccac tcgaacctgg gctaccagtg cgccggggcc
     1801 aggatgaaca acaagctggt gccgctcgat acccctcttg agaacggcgc ggtggtgtcc
     1861 atcctcaagg acaagaaccg ggtggggcct tcgcgcgact ggttgcagtc ggaccgctat
     1921 ttgaagacgg ctaacgcccg ccagaaggtg cgccagtact tccgcaggca gcggcgcgac
     1981 gagaacgtca tgcagggcct gggcatactg gaccagacgc tcaagcacct ggggctgctc
     2041 cacgtgtcgc atatggacgt gctggcctac ttcccccgct acaacagcct ggaagatttc
     2101 cttgaggcta tcggcaacac cgacattacg ccccagaaca taacgtccaa gctgggtgag
     2161 ttccgccagg aggagctgct acagagcacc gacgcgctgc ctgtgagcgt gccgacgagc
     2221 gtctccttgc ccagcatgcc gaccaccatg tcggtaagcg acctgggggg catactcacc
     2281 acgctgggcc gctgctgcaa gcctatgccg ggcgacgaga ttatcggcta cacgaccaag
     2341 cttcggggta ttacggtgca ccgggccgac tgcccgaacc tgcaaacggt gcaggacacc
     2401 gggcgcttgg tgcgcgtgtc gtggggtgcc aaggggctga ccaagtaccc ggcgggcgtg
     2461 cgcattcagg cactcgaccg cgtgggcctg ttgcgcgacg taaccacggt gctcgccgac
     2521 gagaaggtga acctaatcag catccacacc aacaagaccc ccagcgtagc taccagcacg
     2581 atcatcatga ccctggacgt gacgggcgta gaccaactct atcatgtgat gggccgcctc
     2641 agcactgtac ggggcgtata cgaagtaacg agagatacgg gacaagaata gttacgaatc
     2701 gcagtacttt aagcctaatg cgtggttcgt aattgatgaa ggggagggaa aatgccggat
     2761 agagtggctt ttaccgtgtt tggaataccc gtgcattggt acggcattat gagaatgctg
     2821 ggggtgctcg cggcgtctta cgtggtctat atagaggcac gcaggcgcgg ggaggacacc
     2881 gaccacgtgt ggcagttgtt cccatgggcg ctgattgcgg gcatcctggg ggcgcgtatc
     2941 ggctgggtgg tgtcggaggc gggcaaccgg cggttcaacg acttctggga gatattcgcc
     3001 atccagcagg gcggcctgag catccagggt acgatcatcg gcggggcgct ggcggtcgta
     3061 atctactgct ggcggtacaa gctgaggttc ttcaagtggt tggacatgat cgcgccggga
     3121 gtggcgttgg ggcaggccat agggcgctgg ggcaactact tcaaccagga ggcgtttggg
     3181 tcgcccacca acctgccctg gggcataccc atagacgtgc gccgccaggc agaggtggcc
     3241 gggcctaacg ccggtttcgc cccaagctcc gacctgcgct tccacccgac gttcgcctac
     3301 gagatgatct ggaacctgtt caacttcggg ctgttgatgt ggctgggtag gcagcggcgg
     3361 atacggctgc gcgagggcga catcttctgg gtgtacctca tcttctactc ggtaggccgc
     3421 tacgcgatag aggagttgcg cgtggatagc gcgatagtga gcgggttcaa ggcccctcaa
     3481 ctcttctcca tcgtggccat cctgttcgcg ctagtcatga tcgccgtgcg ccacagcccg
     3541 aacagcaagg tgccgctatc ggccaccaac ctgccgcacg gggcatccgt cgcgacacat
     3601 ggccgtacca gggccgcagc gctagatgcg ggcgcagaga gcgcgactcc tcgcgtgcgc
     3661 agggtggcca gaagctcgcg acgcggtcga ggggccacga ctaccgagac tagcggggcg
     3721 ggtacggagt gatagggctt ttctaaacgc caagacgcca agaacgcgcc aaggttaagg
     3781 attattaggg gtggtgcaca agccacccct ttgctattaa cggtgctcag ggtgtccagc
     3841 ggacgtaagc cccgccaacg agccgggggg cgtattggct tgcgc
//
LOCUS       DASRWZ010000788         8349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1228973, whole genome shotgun sequence.
ACCESSION   DASRWZ010000788 DASRWZ010000000
VERSION     DASRWZ010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228973"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(481..1974)
                     /gene="pyk"
                     /locus_tag="VFH60_12515"
     CDS             complement(481..1974)
                     /gene="pyk"
                     /locus_tag="VFH60_12515"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET6314649.1"
                     /translation="MPPRTKIVATIGPACWDEPILRSLLASGVSVARFNFSHAEHART
                     AETFALIRRLADEAGLNVAILADLQGPRVRVGDLPPGGVTLTPGTSLTLDTRNPYYSP
                     GIIPVDYSGLAQDVYPDDLILLDEGLIALKVVDVAPEDGCIVCNVAVGGVLTSNKGLN
                     VPNRPLGVPTITGKDRADLSFALEHGADMVALSFVRTGSDITEARKLIAGTTGRYVPI
                     IAKIEKASAVDNFAEILAEADGVMVARGDMGVEMPPEVLPAIQKRIIAAANIAAKPVI
                     TATQMLDSMIRNPRPTRAEATDVANAVLDGSDAIMLSGETATGKYPLEAVQTMARIAL
                     EAEKLFNYDGWTDHLAGIAPNHNISPQDNRQTAEIICQAADQISDRLNARAVIALTRT
                     GTSARLVSKYRPRSNLIAVTDRPNTCKALAVTWGARVLLLDHFTDTFATMSAAEQAAL
                     QQGLIEPGDLLVFIGGLGQPLAGHTNLLQVQVAGTTSEYPEPNEPAQ"
     gene            complement(2044..3996)
                     /locus_tag="VFH60_12520"
     CDS             complement(2044..3996)
                     /locus_tag="VFH60_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314650.1"
                     /translation="MFGLPPLLAHIAELLALTLLVSIAAGTGLALLRLLRVAPLLTVG
                     ERLLFGTLLGYGTIALLVLGVGLLGLLYTPVVLIILAALAATGFLPLTGALREATPAL
                     HRALNSLRYPPNLFLASLSALSVAAALAKALVPVATQDDLMYHLALPRRYVEQHAINF
                     YPDSNYSLFPQIMEMLYTVGLLLGSDRLSVLFAFTTGLLAAASAALFAKRLMAERHAH
                     PLWQALPLLVAAIFLSAPLHNFIMRAANTDFAQATAEMLGIYAFYLAITARPHVSTRL
                     LALSGICGGLSFSVKYYGLLLPGALGLALLAILLTRWRNADPTGRKAITQSALQNVLA
                     YGLPILAIGGVWLLRNFIASGNPVWPALGNLLGGSFWSPAADPEVLLGTIPGLKWESL
                     PSGLMYQFGATIARKPLPIDNHTYVVSLGPLLLTSLLALPLARWKPPLRLVFYSAAFV
                     WVVSLFYFSRASIRYLATVFLLCALMGAYGLISLAVRARLATFVLGTVVAISLTLVTL
                     DTTLSTRPFLPTTLSLDRFDEELYLQAHMDDYPIMQYINFATPPNALIYDWDSRPRGY
                     RIPRPYVYGRLVPKYSGVNPNPDEWHARLKELGVTHVLVHNRQVFAPGYPPGYDPDRE
                     TFQALATRYLGPPLITEGNFALYELR"
     gene            complement(4471..5265)
                     /locus_tag="VFH60_12525"
     CDS             complement(4471..5265)
                     /locus_tag="VFH60_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6314651.1"
                     /translation="MDQYLQSNRDLWNAWTELHERSEMYDVEGFKKGNFRLHPIEKQE
                     LGDVRGKTMLHLQCHFGLDTLSWANLGAIVTGVDFSEKAIALARKLSEETGIPATFVC
                     SNVYHLPDNLEGQFDLVFTSYGVLGWLPDIDRWAKVVAHFLKPGGTFYIAEVHPVVWI
                     WDSDHPDTLVRQFSYFEKEPLRFETVGSYASAEDYRGVEYGWNHTLSDYINGLIKAGL
                     TIQQFNEYPYMTWKAFPWMVEDDQHYWRMPEGQESIPLIFTLKATK"
     gene            5773..>8349
                     /locus_tag="VFH60_12530"
     CDS             5773..>8349
                     /locus_tag="VFH60_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET6314652.1"
                     /translation="MQSPQDEGVKVRVASSVGAIEKPPLPVAWTRPLPRLHPKHAGGH
                     GRRPLFVLSSARRRKARLTGARGGYYARLPKRRVVPGFVKWFMAVSSSLVLLVMFLVV
                     LSLGSVGGAYLWITKDLPSVDNVHAGGIQFETSRIFDRYGNLLYELYDTEMGKRTYVN
                     IERMPQDLINATIAVEDSSFQENNGVDPLGIIRAAYINYTNQGTSGGSTITQQVVRRI
                     LLPEKDEKTLLRKLREAILAVRMTEKYPKEKILEIYMNEIYYGSLSYGVAAAADTYWG
                     RQVKDLSLAQAAMLAGLPQAPGEYDLNVNFDLAKLRQKIVLDLMVKNEFVTQEQADLA
                     YAEDIRPRPLPAHVAADAPHFVEYARQMLIEKYGVEVALRGGLRVVTTIDLEWQAEAE
                     KIAAQQIQNLRRQGASNAGLVAINPRTGEILAMVGSVDYSNPVYGQVNVATSLRQPGS
                     SFKPITYAAALERGDYNPYTILPDLPVKYANGTSMAYIPRNYDGRFHGPVTMKAALAN
                     SFNIPAVKVLEDVGMPAVLDLAHRMGITTLNDPERYGLSLTLGGGEVRLVDMTAAFAT
                     FANYGYRVPATPFLKVVDGDGKVLEEVDLERPVGEQVLNPGVAFQISSMLSDNQARTP
                     IFGANSPLHIPGIEAAVKTGTTDDSKDAWTMGYTPALAVGVWVGNNDGRRMGNVAGAI
                     GAAPIWHNFIKKVYSDPKLKARLYAPGRDSVEEHFAPAPGMIRAAVCAVSGKAPTSAC
                     THLRHEYVTPENLPTEECDWHVWVPVTLHDGGAHLVGEGVPEEDVIERIYTVPPKEYR
                     GWIGGGPPGTMLVMTETAQVPVPVPTPVEGEPASIAALVTPLPSEARGQAGGGAGLAS
                     ENP"
BASE COUNT         1511 a         2102 c         3173 g         1563 t
ORIGIN      
        1 ggtgctagat gacctgcgat ggtgcaatac aagcggcaca tggtgccagc cgacgaccag
       61 cccagtagtg ccatacaaac cacatagatt ggtagccgac gtgcggagat gcaatacaag
      121 ccacacctgg tgccagccga cgcccggcga ttgaaatcgc gggctacacg atgcaaagtc
      181 cgcccttcgg cggactcctt aagccatata ttcacctaaa agaaactcaa tgcagtagaa
      241 ccagtccccg aagggggact tcgtaggtgt agcccgcgat ttcaatagcc gggcgtcggc
      301 tggcaccagg tgtggcttgt attgcaagct gtcgtaagaa caaggctagc gtgctcaaga
      361 gaaaggggct atttgggtgc tgagggacga gattcttcac tgcgttcaga atgacaaagg
      421 ggcgttcggg gtgctataga agaggctatg tgcaagtacg atggggttgt gcctgataag
      481 ctattgggcc ggctcgttgg gttcagggta ctcgcttgtg gtgccggcta cctgcacttg
      541 taggaggttg gtgtggcccg cgaggggctg gccgaggccg cctatgaaga cgaggaggtc
      601 gcctggctcg atgaggccct gttggagggc ggcttgttcg gcggcggaca tggtagcgaa
      661 ggtgtcggtg aagtggtcga gcagtagcac gcgcgcgccc caggtaacgg cgagggcttt
      721 gcaggtgttg gggcggtcgg tgacggcgat gaggttgctg cgggggcggt acttggagac
      781 gaggcgggcg ctggtgccgg tgcgggtgag ggcgattacg gcgcgggcgt tgaggcggtc
      841 gctaatttgg tcggcggcct ggcagatgat ttcggcggtc tgccggttgt cctgtggcga
      901 gatgttgtgg ttgggggcta tgccggcgag gtggtcggtc cagccgtcgt agttgaacag
      961 cttttcggct tcgagggcga tgcgggccat ggtttgcacg gcttcgagag ggtacttgcc
     1021 ggtggcggtc tcgccggaga gcatgatagc gtcgctgccg tcgagcacgg cgttggccac
     1081 gtcggtggct tcggcgcggg tggggcgggg gttgcgtatc atcgagtcga gcatctgggt
     1141 ggcggtgatg acgggcttgg cggcgatgtt agcggcggcg atgatacgct tctggatcgc
     1201 gggcagcacc tcagggggca tctccacgcc catatcgccc ctggcaacca tcacgccgtc
     1261 ggcctcggcc agtatctccg cgaagttgtc taccgccgag gctttttcga tcttggcgat
     1321 gataggcaca tagcggccgg tcgtgccggc gatcagcttg cgggcctcgg tgatgtcgct
     1381 gccggtgcgc acgaaggaga gggctaccat gtcggccccg tgttccaggg cgaaggagag
     1441 gtcggcgcgg tctttgccgg tgatggtggg gacgccgagg gggcggttgg gcacgtttag
     1501 gcccttgttg gaggtaagca cgccccccac cgccacgttg cacacgatgc agccatcctc
     1561 aggggctaca tccaccactt tgagggcaat gaggccctcg tccagcagga tgaggtcgtc
     1621 ggggtaaacg tcctgggcca gccccgagta gtccacgggg atgataccgg ggctgtagta
     1681 ggggttacgg gtgtccaggg tgagggaggt acctggggtg agggtcacgc cgccgggggg
     1741 gaggtcgcca acgcgcacgc gggggccttg caggtctgcc aggatggcga cgttgaggcc
     1801 cgcttcatcg gcgaggcggc ggatgagggc gaacgtctcg gcggtgcggg cgtgctcggc
     1861 atgtgagaag ttgaagcggg ctacgctgac cccggaggcg agcagggacc gcagtattgg
     1921 ctcgtcccaa caggcgggac cgatggtggc tactattttg gtgcgtggag gcatgggtct
     1981 ccggatttta gattttggat tttgggtttt ggatttattg agtcttatta taagggctgg
     2041 tggctagcgg agttcgtaga gggcgaagtt gccttcggtg attaagggtg ggccgagata
     2101 gcgggtggcc agggcctgaa aggtctcgcg gtcggggtcg tagccgggtg ggtagccggg
     2161 ggcgaatacc tggcggttgt ggacgagcac gtgggtgacg ccgagttcct tcaggcgggc
     2221 gtgccactcg tcggggttgg ggtttacgcc gctgtatttg gggacgaggc ggccgtagac
     2281 gtagggacga gggatgcggt agcccctggg gcggctgtcc cagtcgtaga taagggcgtt
     2341 gggcggggtg gcgaagttga tgtactgcat gatagggtag tcgtccatgt gggcctggag
     2401 gtagagttcc tcatcgaagc ggtctaagga gagagtggtg ggcaggaagg ggcgggtgct
     2461 cagggtggta tccagggtga ctagggtcag ggagattgct acgaccgtgc cgaggacaaa
     2521 tgtggctagc ctggcgcgga cggcgaggga gatgaggccg taagctccca tgagggcgca
     2581 aagcaggaag acggtggcga ggtagcggat gctggcgcgg gagaagtaga agagggagac
     2641 gacccacacg aaggccgccg agtagaagac caggcgcagg ggcggcttcc agcgggcgag
     2701 ggggagggcc agcagcgaag ttagcagcag cgggccgagg cttacgacgt aggtatggtt
     2761 gtctatgggg agcggcttac gcgctatcgt ggccccgaac tggtacatca ggcccgaggg
     2821 gaggctttcc cacttgaggc cggggatggt gccgagcagc acttcagggt cggcggcggg
     2881 tgaccagaag ctgcccccca ggaggttgcc cagggcgggc catacggggt tgcccgacgc
     2941 gatgaagttg cgcaggagcc atacgccgcc tattgccagg atgggtagtc cgtaggcgag
     3001 gacgttctgg agcgcgcttt gggttatggc tttacgcccg gtagggtcgg cgtttcgcca
     3061 gcgggtgagg agtatagcca gcagggcgag gccgagggcg ccgggcagca gcaggccgta
     3121 gtacttgacc gagaaggaga ggccgccgca gatgccggag agcgccagca gtcgcgtgct
     3181 gacgtgcggg cgggcggtga ttgcgagata gaaggcgtag atgcctagca tctcggcggt
     3241 ggcctgggcg aagtcggtgt tggcggcgcg catgatgaag ttgtgcaggg gcgcgctcag
     3301 gaagatggcg gcgacaagca acggcagggc ttgccagagc gggtgggcgt ggcgctcggc
     3361 catgaggcgt ttggcgaaga gggcggcgga ggcggcggcc aggaggccgg tggtgaaggc
     3421 gaagaggacg gagaggcggt cgctgcccag gagcaggccg acagtgtaca gcatctccat
     3481 aatctggggg aagaggctgt agttgctgtc ggggtagaag ttgatggcgt gctgctcgac
     3541 gtagcggcgg ggtagggcga ggtggtacat caggtcgtct tgcgtggcga cgggcaccag
     3601 ggccttggcg agggcggcgg ctacggatag cgcgctgagg ctcgccagga agaggttggg
     3661 cgggtagcgg aggctgttga gggcgcggtg gagggccggt gtggcctctc gcaaggctcc
     3721 tgtgagaggt aggaagccgg tggctgccag ggccgccagt atgattagca cgacgggggt
     3781 gtacagcagc cccagcaggc ccacgcccag cacaagcagc gctatcgtgc cgtagccgag
     3841 cagggtgccg aacagcaggc gctctcccac cgttagcagg ggtgccacgc gtaggaggcg
     3901 caacagggcc aggccggtgc ctgcggcgat gctcaccagc agggtgaggg cgagcagttc
     3961 ggctatatgg gcaagcagcg ggggcagacc gaacatgcta gccggacagg ggtatcaggt
     4021 tgggcaggtt ctgccactgg aagacgtaga ggtagagcat cgcgaggagc acgatggcca
     4081 ggaggagggt atagatgttg gctagagtgt ctttcatggg tgtttcagcg gtggaacgtg
     4141 gtgcgagtgt atgttcttgg tgtgtgaata tgatgcagtt ggtgtatcgg ggccaaggct
     4201 gccttttgtc actctgagcc cttcggctgc gctcagggta aactccgcga agaatctcgt
     4261 cctctatcga gatgttacac ctttcttcag agagaaagag ggtaacccgg taagaaaggt
     4321 ggtatttggg tgctggagga cgagattctt cgctgcgctc agaatgacag agggggagtt
     4381 ggtcccgcta aagcagctta gtgcattcac gtcttgtccc tcgcgtaggt ctgcatttag
     4441 gtgttgtgtt tgatggtagg gtagtgttcc tcatttggtg gctttgagtg tgaatatgag
     4501 gggtatcgac tcctggcctt cgggcatgcg ccagtagtgt tggtcgtctt ctaccatcca
     4561 ggggaaggct ttccaggtca tgtacgggta ttcgttgaac tgctgtatgg tgaggccagc
     4621 tttgatgagg ccgttgatat agtcggagag ggtgtgattc cagccgtatt ctacgcccct
     4681 gtagtcctcg gcgctggcgt aggagcctac cgtctcgaag cggagcggct ctttctcgaa
     4741 gtaggagaac tggcggacca gggtatcggg gtggtcggag tcccatatcc agacgaccgg
     4801 gtggacttcg gcgatgtaga aggtgccgcc gggcttgagg aagtgggcga ccactttagc
     4861 ccagcggtcg atgtcgggca gccagccgag cacgccgtag gaggtgaaga ctaagtcgaa
     4921 ctggccttcc aggttgtctg gcaggtggta gacgttggag cagacgaagg tggccgggat
     4981 gcccgtttct tcgctcagct tgcgggcgag ggcgatggct ttctcggaga agtcgacgcc
     5041 ggtgacgatt gcgcccaggt tggcccagga gagggtgtca aggccgaagt ggcactggag
     5101 gtggagcatc gttttgccgc gcacgtcgcc aagctcctgc ttttcgatgg ggtgcaggcg
     5161 gaagttgccc ttcttgaagc cttcgacgtc atacatctcg gaccgttcgt gtagctcggt
     5221 ccaggcgttc cagaggtcgc ggttggattg gaggtactgg tccattttgt cctcttgtgc
     5281 tgcgatggtg ccctccgggg acgccctgtg gctaaagccc cagggcgtca tgctgggcct
     5341 cacctgccta gattgccggt ataagtactc ccctacgccc ttatgatgta tattacatta
     5401 tgtcgcagcc gcaataacgt gtgagtggcg gctacggcag gtattatagc gtaatcggcg
     5461 gatgaaggca tgactgctgc tatcgtttgg agtgcgcggg actatcgcag tctcatacga
     5521 ggtacttttt cgtcatataa agtggccgtg tttggttgta tgctttctat gtagcttatg
     5581 cgtgtggccc ctcgacaacc tgcgcacacc agcccaccgc gacgcctgcc caggtcgtcc
     5641 cgcgcgcttg tggctgtccg cgataggata aagggcaggg cggttgagcc gtccgcggca
     5701 gctacgccct cggttgtttc cccaaagaca ggtactttgt tgccggcgcg cgctcatgcg
     5761 gcgaaggctc gcttgcagtc cccgcaggac gagggagtga aggtgcgggt agcctcgtcg
     5821 gtgggggcga ttgagaagcc gccgctgccg gtggcgtgga cgcggcccct gcccaggctg
     5881 cacccgaagc atgcgggcgg gcatggcagg cggcctcttt tcgtgcttag ctcggcccgg
     5941 cgacgcaagg ccaggctcac gggagcgagg ggagggtact acgcgcggct gccgaagcgc
     6001 cgggtggtgc ctgggttcgt gaagtggttc atggcggtat cgagcagcct ggtgttgctg
     6061 gtgatgttcc tggtcgtgct ctcccttggt agcgtgggcg gggcgtacct gtggatcacc
     6121 aaggacctgc cctcggtgga taatgtacac gcgggcggga tacagttcga gacgagccgc
     6181 atcttcgacc gctacggcaa cctcctctac gagctgtacg acacggagat gggcaagcgc
     6241 acttatgtca acatagagcg gatgccgcag gaccttatca acgccactat agcagtggag
     6301 gactcgtcgt tccaggagaa caacggggtg gacccgctgg gcataatcag ggcggcgtac
     6361 atcaactaca ccaaccaggg gacgtcgggc ggctctacga tcacgcagca ggtggtgagg
     6421 cggatattgc tgccggagaa ggacgagaag acgctcctgc gtaagctacg cgaggcaatc
     6481 ctggcggtgc gcatgacgga gaagtacccg aaggagaaga tactagagat ctacatgaac
     6541 gagatctact acggatcgct ctcctatggc gtggcggcgg ccgccgacac gtactggggc
     6601 aggcaggtga aggacctgag cctggcgcaa gcggcgatgc tggcggggct accccaggca
     6661 ccgggcgagt acgacctgaa cgtgaatttc gaccttgcga agctccggca gaagatcgtg
     6721 ctcgatttga tggtgaagaa cgagtttgtc acgcaggagc aggcggacct agcttacgcg
     6781 gaggatatac ggccgcgacc gctgccggcg cacgtagcgg ccgacgcccc gcacttcgtg
     6841 gagtacgccc ggcagatgct aattgaaaag tacggggtgg aagtggcgtt gcggggcggg
     6901 ctgcgggtgg tgaccacgat agatttggag tggcaggccg aggccgagaa gatcgcggcg
     6961 cagcagatac agaacctcag gcggcaggga gcgagcaacg cggggctggt ggctatcaac
     7021 ccgcgcacgg gcgaaatcct ggcgatggtc ggctcggtgg actattcgaa cccggtgtac
     7081 gggcaggtga acgtggctac gtcgttgcgg cagccggggt cgtcgttcaa gccgattacg
     7141 tacgcggcgg cattggagcg gggggactac aacccctata cgatcctgcc cgacctgccc
     7201 gtgaagtacg ccaacggcac ctcgatggcg tacatacctc gcaactacga cgggcggttc
     7261 cacgggccgg tgacgatgaa ggcggcgctg gctaactcgt ttaacatccc ggcggtgaag
     7321 gtgctggagg acgtgggcat gcccgccgtg ctcgacctgg cgcaccgcat gggtatcacc
     7381 acgctgaacg acccggagcg gtacgggctg tcgctcacgc tggggggtgg cgaggtcagg
     7441 ctggtggaca tgacggcggc gttcgccacc tttgccaact atgggtacag ggtgcccgcc
     7501 acgccgttcc tgaaggtggt ggacggggat ggcaaggtgc tggaagaggt cgatttggag
     7561 cgcccggtcg gggagcaggt gctcaatccg ggggtggcct tccagatcag ctccatgttg
     7621 tcggacaacc aggcccgcac gccgatattc ggggccaact cgccgttgca cataccgggg
     7681 atagaggcgg cggtcaaaac gggcaccacg gacgactcga aggacgcctg gacgatgggc
     7741 tacactcccg cgctggcggt tggggtgtgg gtgggcaaca acgacggcag gcgcatgggg
     7801 aacgtggcgg gggctattgg cgcggcccct atctggcaca attttattaa gaaggtatat
     7861 agcgacccca agctgaaggc gagactgtac gcgcctggca gggactcggt ggaggagcat
     7921 ttcgcgcccg cgcctgggat gatacgggcg gcggtgtgcg cggtgtcagg gaaggccccg
     7981 acgagcgcct gcacccacct gcggcacgag tacgtcacgc cggagaacct gcccacggag
     8041 gagtgcgact ggcacgtgtg ggtgccggtg acgctgcacg acgggggcgc tcacctggtg
     8101 ggtgaggggg tgccggagga ggacgtgata gagcggatat acacagtgcc gccgaaggag
     8161 tacaggggtt ggataggggg cgggccgcca ggtacgatgc tggtgatgac ggagaccgca
     8221 caggtgccgg tgccggtgcc tacgcccgtg gagggagagc ctgcctcgat agccgcgctg
     8281 gtgaccccgc tgcccagcga ggcgcgaggg caggcgggcg gcggggcggg gctggcatcg
     8341 gagaacccg
//
LOCUS       DASRWZ010000789         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1022588, whole genome shotgun sequence.
ACCESSION   DASRWZ010000789 DASRWZ010000000
VERSION     DASRWZ010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022588"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VFH60_12535"
     CDS             <1..1273
                     /locus_tag="VFH60_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314653.1"
                     /translation="NQQRNMGRGSLYFPETGKWLGGEFLEFWQANGGLAQQGYPITDE
                     FTETSALNGQQYRVQYFERSVMELHPENAPPHNVLLAQLGRFRYDELYGPARATGSPP
                     TDQLVARNVMGRVVANDRYLFWIANTPPNFPIHGFDIRENRSFLVTDAGGYKYDLACD
                     NSALAWVEMNKDAYPPASGRPVPVRIMKFEPARGKVSIVREGRMSDQIPEGRAAVRDW
                     MLYYNERSGGIRAQSLSTGHEYFFLADAHDPVVSGDVVLWSRRLPECLQGDGKPLCIG
                     AQELYVRKGTQDTLLAKIKGQFTFEQYAVHDDKVVWTNSPIGGSGAPPQLYSLSAGTT
                     ITMSQQLARFPVVRDDLVLWGSAPAAGKDWSIDRYYPASGAITVLLQSATPMYPQAFI
                     GREALAYIAGKESPKAGYRTTGDLYLLRLGP"
     gene            complement(1275..>2709)
                     /locus_tag="VFH60_12540"
     CDS             complement(1275..>2709)
                     /locus_tag="VFH60_12540"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET6314654.1"
                     /translation="VQTLNLDEFRNYNRLSLQLSPGMTVLQGDNASGKTSLLEAVYLL
                     ATTRSPRATSDLELVNFAAPNDLGSPPFARITAHVERARGPLAVEILIVRDEEPAGPG
                     GLAGTVQARKRIKVNGVARRALDLVGQATVVLFTPQDVDLVTGPPSLRRKYMDVTISQ
                     MDHRYVRLLAQYMKVLAQRNSLLRALKEQGRGAGSRDVLDEMAYWDEELVRHGAYVVL
                     RREVFAARLSQRAEAVHHTLAGTDAVNGRANTRQLLRERRPTAELRLNVAYQSAVAPE
                     ARKLLIPELVAQMEAESALSDLGNDETTAALDPAARGRIPHLDRHIERISEEFKRQLD
                     EVRANEVRRGMSVLGPHRDDIVFQLGDPSAGSGQAPTSSPGQALPLNAYGSRGQQRTA
                     VLALKLAEVDLMTAETGDTPILLLDDILSELDANRRGYLLNAVSSQQYQSLITTTDLA
                     GFDPKFLERATLLEVRQGEVLAMEAAS"
BASE COUNT          490 a          820 c          937 g          462 t
ORIGIN      
        1 taaccagcag cggaacatgg gcaggggctc cctctacttc cccgaaacgg gcaaatggct
       61 gggaggcgag ttcctcgaat tctggcaggc gaacggcggc ctggcccagc aaggctaccc
      121 tatcacagat gagtttaccg aaacgtcggc gctgaacggc caacaataca gggtgcagta
      181 cttcgagcgg tccgtcatgg agctacaccc ggagaacgcg ccgccgcata acgtgctgct
      241 cgcacaactc ggcaggtttc gctacgatga gctgtatggg ccggcacgcg ctaccggcag
      301 ccctcctacc gaccaattgg tagcccgcaa cgtcatgggt agggtggtgg caaatgacag
      361 gtacctgttc tggattgcca acaccccgcc aaacttcccc attcacggct tcgacatacg
      421 ggagaataga agctttctgg tcacggacgc gggcgggtac aagtacgacc tggcgtgcga
      481 taacagcgcc cttgcctggg tagagatgaa caaggatgca tacccaccgg cttcgggccg
      541 gccggtgccg gtacgcataa tgaagttcga gcctgccagg gggaaggtgt ccatcgtgcg
      601 cgaaggcagg atgtcggacc agataccgga gggccgggcg gcggtgcgag actggatgtt
      661 gtactacaac gagaggtcgg gcggcatccg agcgcagagc ctgagtacgg gccatgagta
      721 cttctttctt gcggatgcgc acgatccggt ggtttcagga gacgtagtac tgtggtcccg
      781 gcggctaccc gagtgccttc agggcgacgg caagcccttg tgcatcggcg cccaggagct
      841 atacgtcaga aaaggtacgc aagacaccct gctggcgaag ataaagggcc aattcacctt
      901 cgagcaatac gccgtgcacg acgacaaagt agtatggacc aacagtccca taggggggtc
      961 tggcgctcca ccgcaattgt atagtctcag cgcgggcacg acgatcacca tgtcccagca
     1021 gctcgcccgc ttcccggtgg tgagggatga cctggtcttg tggggaagcg ccccggccgc
     1081 cggtaaggac tggtcgatag accgctacta tccggcgagc ggagcaatca ccgtgctatt
     1141 gcagagcgcg acccctatgt acccgcaggc cttcatcgga cgcgaagccc tggcctatat
     1201 cgcgggcaag gaatcaccta aagccggtta ccgcaccacg ggcgacctct acctgctgag
     1261 gctgggaccg tagcctaaga agcagcctcc atcgcgagca cctcgccctg ccgcacctcc
     1321 aacagggtgg cgcgctcgag gaacttgggg tcgaagcccg ccaggtcggt cgtggtgatg
     1381 agcgactggt attgctgcga gctgaccgcg ttgagcaggt agccccggcg gttggcgtca
     1441 agctcggaga ggatgtcatc gaggaggagg atgggggtgt cgccggtctc ggcggtcatc
     1501 aggtccacct cggcaagctt gagggcgagc acggcggtgc gctgctggcc ccttgagccg
     1561 taggcgttaa gcgggagggc ctgccctggg ctggaggtgg gtgcctgccc tgagcctgcc
     1621 gaagggtcgc ctagctggaa cacgatgtcg tcgcggtgcg gccccagcac cgacatgccc
     1681 cgcctcacct cgttagcgcg cacctcgtcc agttggcgct tgaactcctc ggagatgcgc
     1741 tctatatgcc tgtccaggtg gggtatcctg cccctggcag cggggtcgag ggcggcggta
     1801 gtctcgtcgt tgcccaggtc ggagagggcg ctctcggcct ccatctgggc cacgagttcg
     1861 gggatcaaca gcttgcgggc ttcgggggcc acggccgact ggtaggccac gttcaggcgc
     1921 aattcggcgg tagggcggcg ctctcgcagc agttggcggg tgttggcgcg gccgttcacg
     1981 gcgtccgtgc ccgcgagcgt atggtgtacc gcctcggcgc gctgggagag gcgggcggcg
     2041 aacacctccc ggcgcagcac cacgtaggcc ccgtgccgca caagctcctc gtcccagtaa
     2101 gccatctcat ccagcacgtc gcgcgaccct gcgccccgcc cctgctcttt gagcgcgcgc
     2161 agcagcgagt tgcgctgggc gagcaccttc atgtactggg cgagcaggcg cacgtagcgg
     2221 tggtccatct gcgagatggt tacgtccatg tatttgcggc gaagggaggg ggggcctgtc
     2281 acgaggtcca cgtcctgcgg ggtaaagagc accacagtag cctggcccac gaggtcgagg
     2341 gcgcggcgag ccaccccgtt caccttgata cgcttgcggg cctgcaccgt gcccgccagc
     2401 ccgccaggcc cggcaggctc ctcgtcgcgc acgatgagta tctcgaccgc cagcggcccc
     2461 cgcgcgcgct cgacgtgggc cgtgatgcgg gcgaaggggg gcgagccgag gtcgttgggg
     2521 gcggcgaagt tcaccagttc caggtcggag gtagcgcgtg gggagcgggt ggtagccagc
     2581 aagtagacgg cttcgaggag cgaggtcttg ccggaggcgt tatcgccttg cagcacggtc
     2641 atgccggggg atagctgcag ggagaggcgg ttgtagttgc ggaactcgtc taagttgaga
     2701 gtttgaacg
//
LOCUS       DASRWZ010000790         4285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_475008, whole genome shotgun sequence.
ACCESSION   DASRWZ010000790 DASRWZ010000000
VERSION     DASRWZ010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475008"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..330
                     /locus_tag="VFH60_12545"
     CDS             100..330
                     /locus_tag="VFH60_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006528754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET6314655.1"
                     /translation="MIEDSEAQLLNRITVNPEIFGGKPIIRGRRLAVEHILGMLAAGD
                     TQGTLLREYPWLEPKDIQACLVYAKHSLEGQE"
     gene            353..1912
                     /locus_tag="VFH60_12550"
     CDS             353..1912
                     /locus_tag="VFH60_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6314656.1"
                     /translation="MQTIAPETQLPAPSAEFALQARGITKRFPGVLANDHIDLDLRPG
                     EVHALLGENGAGKSTLMNILYGLYRPDEGAIYIKGKETHFTGPSHAIAAGLGMVHQHF
                     MLVPPLTVTENIMLGGETTQRGVMLDKDAAARRIAELSATYGLQVDPNATIEDLPVGS
                     QQRVEILKAFYRNADVLILDEPTAVLTPQEARDLFVIMRRLAESGKAIVFITHKLKEV
                     LAIADRITVLRHGRVAGTADPRASSEAQLASLMVGRSVLLEVKKKPANIGEAVLSIEG
                     LEVKDNRGHLGVDGLSMQVRAGEIFGIAGIEGNGQTELVEALTGLRKVHRGKIMLDTR
                     DVTLTGTRQLIDQGLAHVPEDRHKHGLVLTYSVADNICLSTYDERPFASGPLRNDNFI
                     FSFARKLVKKFDVRTASVQTQARNLSGGNQQKAILARELSRQIRLLVASQPTRGLDVG
                     SIEFIHKQIVAQRDEGVAVLLVSAELDEVLALSDTVGVIYKGKLVAVMPRAEATREKL
                     GLLMTGGTVPA"
     gene            1935..3401
                     /locus_tag="VFH60_12555"
     CDS             1935..3401
                     /locus_tag="VFH60_12555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET6314657.1"
                     /translation="MHPASTPDWQETRARYDVALRWLEALITDPKGERYLLPQTQEEF
                     RRSQEAGIARTSRFLDYLGNPQQAYRTVHVAGTGGKGSVATMIAAILRASGANTGLHT
                     SPYLQVPNEKLVANGRMIAPSSMVRLIEQFRPLYEGYIARFPDETPRYLEAWAGITHL
                     FFRHMGVEWAVIETGMGGRFDPTNVGDYDLAVITNVDFDHVPQLGTSLPEIAWHKAGI
                     IKPRKPAITGETKPEALEVIEREAAAHASELFRLGRDYSLENTRHHEHGVITDILTPF
                     GRLADLYVSLLGEFQATNAATASMSAYLLRERHGVAVMEGAIREAMGSLKIAGRMEVV
                     QTEPLVVIDGAHNPQKLRAAASALRQDYPGLEKTLVIGMLRTKDAQSSLAEIVPLADR
                     IIATEARVLGKPSFEAREIAEMARGMRPVVSVTVEPDVQPGIKRAIEDMALDGMVFVT
                     GSIYMLGQARELWHPREELLGRLEHGEGTRLPASGPGP"
     gene            complement(3382..3627)
                     /locus_tag="VFH60_12560"
     CDS             complement(3382..3627)
                     /locus_tag="VFH60_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314658.1"
                     /translation="MGLDAVEVRKIGDWLERTGVQAACPACGSAGTQVPFDVVAAPVK
                     KPSGLHIGDKMTPIVQVVCNNCAHVNFFLAEPMGLDR"
     gene            complement(3689..>4285)
                     /locus_tag="VFH60_12565"
     CDS             complement(3689..>4285)
                     /locus_tag="VFH60_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6314659.1"
                     /translation="LPYITIMPDQNTTPDYPLEEALELTTAEQLKALADPTRQRILGL
                     IGQRAASTKQLSEMLSVPKGTVGHHLKALESAGLVRVVRARQVRAITEKYYGRVARVF
                     RVSSDGGLGGGEGIALSKDVATMPLRQALSEVVLSNDKDDPTTFLISHARIPKSRARE
                     FALRLEAISQEFGVQAAPGEKVFGLVAGVYQTDWRGSD"
BASE COUNT          890 a         1390 c         1293 g          712 t
ORIGIN      
        1 ctacgctcag aatgacaaag gcggggctta gatgccccta tggcacattg tcattcttga
       61 gatagattgt agcgatgact cacgagtgag tgactagaga tgatagagga cagtgaagcg
      121 cagttgctca acaggataac cgtgaaccct gagattttcg gtggtaagcc catcatcagg
      181 gggcgacgcc tggctgtgga gcatatattg ggtatgctgg cggcaggcga tacccaaggg
      241 actttgctta gagagtatcc ctggctcgag ccaaaagaca ttcaagcgtg cctcgtgtac
      301 gcgaaacatt cactggaagg tcaagagtag taaagaacgg tagcccaaac ttatgcaaac
      361 aatagccccc gaaacccaac tacccgcccc cagcgccgag ttcgccctgc aagcgcgcgg
      421 catcaccaag cgcttccccg gcgtgctcgc caacgaccac atcgacctcg atctgaggcc
      481 gggcgaggtg cacgcgctcc tgggcgagaa cggcgcgggt aagtccaccc tgatgaacat
      541 cctctacggc ctctaccggc ccgacgaggg cgctatctac atcaagggca aggagaccca
      601 cttcaccggc cccagccacg ctatcgccgc cggtctcggc atggtccacc agcacttcat
      661 gctcgtgccg cccctcaccg tcaccgaaaa catcatgctc ggcggcgaga ctacccagag
      721 gggcgtgatg ctcgacaagg acgcggcggc caggcgtatc gcggagctat cggccaccta
      781 cggcctgcaa gtggacccca acgccacgat agaggacctg cctgtcggct cgcaacagcg
      841 cgtggagatc ctcaaggcat tctaccgcaa cgccgacgtc ctgatcctcg atgagccgac
      901 cgccgtgctc accccccagg aggcgcgcga cctcttcgtc atcatgcgcc gcctggccga
      961 gagcggcaag gccatcgtct tcatcaccca caagctgaag gaagtgctgg ctatcgccga
     1021 ccggattacc gtgctgcgac acgggcgggt agcgggcacc gccgaccccc gcgccagcag
     1081 cgaggcccag cttgcctccc tcatggtggg ccgctcggtg ctgctggagg taaagaagaa
     1141 gcctgccaat ataggcgagg cggtgctctc gatagagggg cttgaggtga aggacaaccg
     1201 ggggcacctg ggggtggacg gcctcagcat gcaggtgcgc gccggggaga ttttcggcat
     1261 cgcgggcata gagggcaacg ggcagaccga gctggtggag gccctcaccg gcctgcgcaa
     1321 agtgcaccgt ggcaagatca tgctcgacac gcgcgacgtg accctcaccg gcacccgcca
     1381 gctcatcgac cagggcctgg ctcacgtccc ggaggaccgg cacaagcacg gcctggtgct
     1441 cacctattca gtggccgaca acatctgcct gagcacctac gacgaaaggc ccttcgcaag
     1501 cggccccttg cgcaacgaca acttcatctt cagtttcgcc cgcaagcttg tgaagaagtt
     1561 cgatgtgcgc acagcctcgg tgcagactca ggcccgcaac ctctccggcg gcaaccagca
     1621 gaaagccata ctcgcccgcg agttgtcccg ccagatacgc ctgctcgtgg cctcccagcc
     1681 gacgcgcggc ctcgacgtag gctcaataga gttcatccac aagcagatag tggcccagcg
     1741 cgacgagggc gtggcggtgc tgctcgtatc cgccgagctt gacgaggtgc tggccctgtc
     1801 cgacacggta ggtgtgatct acaagggcaa gcttgtagcc gtcatgcccc gcgccgaggc
     1861 gaccagggag aagctcggcc tgctgatgac gggtgggacg gtgcccgcct agcaaacagg
     1921 cggccagagc atctatgcac cctgcgagca cccctgactg gcaagagaca cgcgcccgct
     1981 atgatgttgc cctccgctgg ctcgaggccc tcatcaccga ccccaaaggc gagcggtacc
     2041 tcctccccca gacgcaggag gaattcaggc gcagccagga agccgggatc gcccgcacct
     2101 cccgcttcct cgactacctc ggcaaccccc aacaggcgta ccgcacagta cacgtagcag
     2161 gcacgggcgg caaaggctcg gtcgccacga tgatagccgc catactacgg gcttcgggcg
     2221 caaacaccgg gctgcacacc agcccctacc tgcaagtgcc aaatgagaaa ttggtagcca
     2281 acggcaggat gatcgcccct tcgtctatgg tgcggctaat cgagcagttc aggccgctgt
     2341 acgagggcta tattgcgcgc tttcccgatg agacgccaag gtacctggaa gcctgggcgg
     2401 gcataacgca cctgttcttc cggcatatgg gcgtggagtg ggcggtcatc gagaccggca
     2461 tgggcgggcg gttcgacccc accaacgtgg gcgactacga cctcgccgtc atcaccaacg
     2521 tcgatttcga ccacgtaccc caactcggca ccagcctacc ggagatagcc tggcacaagg
     2581 cgggcatcat caagccccgc aagcccgcga ttacgggaga aacaaagccc gaagctttgg
     2641 aggtaatcga gagggaagcg gccgcccatg cctcggaact gttccgcctc ggccgtgact
     2701 acagcctgga aaacacgcgg catcacgagc acggggttat caccgacata cttacgccct
     2761 tcggaaggct agccgatctc tacgtcagcc tcctgggaga gttccaggcg actaacgcgg
     2821 cgaccgccag catgtcagca tacttgctgc gcgagcggca cggggtggcg gtcatggagg
     2881 gggccatacg ggaggcaatg gggagcttga agatcgcggg gcggatggag gtcgtgcaga
     2941 ccgagccgct ggtggtgata gacggcgcgc acaaccccca gaagctacga gccgcggcct
     3001 cagcacttcg ccaggactac cctggcctcg aaaagacgct ggttatcggc atgttgcgga
     3061 ccaaggacgc gcaatcatcg ctcgcggaga tcgtgccctt ggctgaccgc ataatcgcca
     3121 ccgaggcgcg agtgctgggc aaaccgtcgt tcgaggcccg tgagattgcc gagatggcgc
     3181 gcggcatgcg gcctgtggtg agcgtcaccg tggagcctga cgttcagcca ggcataaagc
     3241 gggcaatcga ggatatggca ctggacggca tggtctttgt tacaggctct atttacatgc
     3301 tgggccaggc gcgcgagcta tggcacccac gcgaggagtt gctcggtagg ctggagcacg
     3361 gcgaaggcac caggctgccg gctagcggtc caggcccata ggctcggcca ggaagaagtt
     3421 tacatgggcg caattgttac acaccacctg cacgatggga gtcattttgt ccccaatatg
     3481 tagcccgctc ggcttcttca caggcgccgc caccacatcg aatggcacct gggtaccggc
     3541 agagccgcac gccgggcacg cagcctgcac ccctgtgcgc tccaaccagt caccaatctt
     3601 tctcacttca acagcatcaa gtcccatctc agtccctccg gcaatcagca attacaaggc
     3661 gctcacaatg atcccaaaat ccaaaatcct aatcactccc ccgccaatcg gtctggtaga
     3721 ctccggccac caggccgaac actttctcgc ctggtgccgc ctggacgcca aactcctggg
     3781 atatggcttc cagccggagg gcgaactccc ttgccctgga cttgggtatg cgtgcgtggg
     3841 agatgaggaa ggtggtgggg tcgtctttgt cgttggagag cactacctcc gatagcgcct
     3901 gccgcagggg catggtcgcc acgtccttgc tgagggctat gccttcgcca ccccccaggc
     3961 cgccatccga ggagacccgg aacacacgcg ccacccggcc gtaatacttc tccgtgatag
     4021 ccctcacctg ccgcgcccgc accacgcgca ccagacccgc gctctccaac gccttcaagt
     4081 ggtgcccgac agtgcccttg ggtacagaca acatctccga caactgcttg gtggaggccg
     4141 cccgctgccc tatcagcccc aggatgcgct ggcgggtcgg gtcggcaagt gcctttagct
     4201 gctcggcggt tgtcagttcg agcgcctctt caagcgggta gtcgggagtg gtattttggt
     4261 ctggcataat agtaatatac ggtaa
//
LOCUS       DASRWZ010000791         3122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_612674, whole genome shotgun sequence.
ACCESSION   DASRWZ010000791 DASRWZ010000000
VERSION     DASRWZ010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612674"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFH60_12570"
     CDS             <1..789
                     /locus_tag="VFH60_12570"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6314660.1"
                     /translation="KLIGEGRTAQVYAWGPRHVLKLYYDWWPRGNVEYEAKVGWAVHA
                     AGVPCPAVGEIVQVDGRRGLVYERVDGPSMVDLLLAEPHRLEERAITFAGLLAAMHHP
                     ARGSGLPGQRERLIRHIDLASPTPLPIELKDKVLQVLSGLPDGAVVCHGDLHPGNVLV
                     SSRGPIAIDWENASLGSPLADVARTALLLETAHFYLPQTPDYPSIADAIALFRRLYLQ
                     AYCAVTGADLGLISAWRAPIAAARLHENIAVEEANLLGIVAEGP"
     gene            complement(797..1681)
                     /locus_tag="VFH60_12575"
     CDS             complement(797..1681)
                     /locus_tag="VFH60_12575"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6314661.1"
                     /translation="MDFRHMQTFQAVVKHASFVRAAEELQYAQSTVTLHIQQLEAELG
                     VELFTRQGKKVKLTEAGRTLREQVDHILGRVDTLRQSMQELGEGEAGHVRIGAIEPTA
                     SRRLPPVLFEFSRQRPKVRLAVEVEGTATISRRVASGDLEFGICSAPAPGLGLVFEPL
                     VREPMGLLVPENHPFAVKERVYVDDLGESRFVLTEPHCAYRQLIQRSLVERGTNVFAG
                     VEIGSVNALVWAVRSGVGVAIVPLESVSPPPEGTVLREIEEIDIALSVGIVQRAGHNI
                     ESRAARELLALIRSCLKR"
     gene            2212..2664
                     /locus_tag="VFH60_12580"
     CDS             2212..2664
                     /locus_tag="VFH60_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314662.1"
                     /translation="MTYPNAEVARLLNAEFIPVRINTQDQPEVARRFHLMWSPILVAL
                     DHREFPLRRIVGYLPPAEMLAQLHFIRALSEMRSARPANALQIFRQLYTRFPNADVVP
                     EAIYWEGIAAYRQSKNKEDLWVIWRRLAAGYPNNAWAARTTLLPEGER"
BASE COUNT          579 a         1044 c          923 g          576 t
ORIGIN      
        1 aaactcatag gcgagggccg caccgcgcag gtatacgcct gggggcctcg tcacgtcctc
       61 aagttgtact acgattggtg gccccgaggc aacgtagagt acgaggctaa ggtaggctgg
      121 gccgtgcacg cggcgggcgt cccctgccct gccgttggcg agatcgtaca ggtggacggg
      181 aggcgcggcc tggtctacga gcgggtagac ggcccgtcga tggtggacct gctcctggcg
      241 gaaccccacc ggctggagga gcgtgccatc accttcgccg gcctactcgc cgcgatgcac
      301 catcccgcac gcggcagcgg cctgcccggc cagcgcgaga ggctgatccg gcacatcgac
      361 ctggcgtcac ccacgcccct ccccatcgag ctgaaggaca aggtgctgca agtgctatcg
      421 gggctgcccg acggtgccgt agtctgccac ggcgacttgc accccggcaa cgtgctcgtg
      481 tcctctcgcg gccctatcgc catcgactgg gagaacgcca gcctcggcag ccccctcgcg
      541 gacgtggctc gcaccgcgct cttgctggag accgcccact tctacctgcc gcagacgccc
      601 gactacccct ctatcgcgga cgcaatcgcc ctttttcgca ggctctacct gcaagcctac
      661 tgcgccgtca ccggcgcgga cctcggcctg ataagcgcct ggcgagcgcc cattgccgcc
      721 gcccgcttgc acgaaaatat agcggtggag gaggccaact tgctcggcat cgtggctgaa
      781 ggcccttaga aggaagctag cgtttcaagc aactgcggat gagggccagt agctccctgg
      841 cggcgcggct ctctatgttg tgcccggccc gctgcactat gcctaccgac agggctatgt
      901 ctatctcctc gatctcgcgc aggacggtgc cctccggcgg ggggcttacg ctctccagcg
      961 gcacgatagc cacccccaca ccgctacgta ccgcccacac cagcgcgttg acgctgccga
     1021 tttcgacgcc cgcgaacacg ttggtgcccc gctccaccag cgacctctgt ataagctgcc
     1081 ggtaggcgca gtgcggctct gtgagcacga accgcgactc ccccaggtcg tccacgtaca
     1141 cccgctcctt cacggcgaac gggtgatttt ccggcaccag caggcccatc ggctcgcgca
     1201 ccagcggctc gaataccagg cccagccccg gcgcgggcgc ggagcaaatc ccaaattcga
     1261 ggtcgcccga ggccacccgc ctgctgatcg tggcggtgcc ctccacttcc accgccaggc
     1321 gcaccttggg ccgctggcgg ctgaactcga acagcaccgg gggcaggcgt ctgctggccg
     1381 taggctcgat tgcacctatg cggacgtgcc ccgcctcgcc ctccccaagc tcctgcatgc
     1441 tctgccgcag cgtgtccacc cggcccagta tgtggtccac ctgctccctg agcgtgcgtc
     1501 ccgcctccgt aagcttcact ttcttgccct gccgggtgaa aagctccact ccaagctccg
     1561 cttcaagctg ctggatgtgc agcgtcaccg ttgactgggc gtattgaagc tcctctgcgg
     1621 cccgcacgaa gctggcgtgc ttcaccaccg cctggaacgt ttgcatgtgc ctgaagtcca
     1681 tcgtcccacc cctgcatcta cttcgataat actgaataaa acttcatatc attcatcttg
     1741 aataatatac cacagcccct tatgcttgaa cagtaagcag cctgccggcg gccgggccac
     1801 acaaaggcca ttgcttcgag caggaggacc aaagggacta ttgtcattcc gaatgcagtg
     1861 aggaatcagg tggcagtcgt acagtgctgc aacaggcgtg atcggtgcga tatacacaaa
     1921 cggataaaac agggccgccc aggtacccca attcaacggc ctcacctacc cacctgattc
     1981 ctcgccgcgc tcggaatgac aatggcctcg tttgggtatc acaaatctaa cgactgctgc
     2041 tcgtgagaag aacaaatggg atatttatcg tcgtgaggcc agatgagtgc tgagcttata
     2101 gagtggtata ccaacctgga gaccgcgatg tcggcggcta gcagggcgtc caggccggtg
     2161 ttcatcgact tctggtcgta cgggtgagcg gggtgcgcgc agctggaggc tgtgacgtac
     2221 cccaacgccg aggtggcgcg gctcttgaac gccgagttca tcccggtgcg catcaacacg
     2281 caagaccagc cggaggtagc gcggcgcttc cacctgatgt ggtcgcccat cctggtggcg
     2341 ctggaccacc gcgagttccc cttgcgccgc attgtgggct acctgccacc cgccgagatg
     2401 ctggcgcagt tgcacttcat ccgcgccctg agcgagatgc gcagcgcacg gcccgccaac
     2461 gccctacaaa tattccggca gctttacacc cgcttcccca acgccgacgt cgtgccggaa
     2521 gctatttact gggaaggcat agctgcgtac cggcagagca agaacaagga ggacctgtgg
     2581 gtaatctggc gccggttagc cgccggctac cccaacaacg cctgggccgc aaggactacc
     2641 ctcttgcctg aaggcgagcg gtaattgcat cttggtgatc ccatgcgttc aaaagtgggg
     2701 gctttgtcgt cctgagtctc tcccgtcagc ctgtattacc ctttgtcatc ctgagccctt
     2761 cggctgcgct cagggtaaac tccgctaagg atcaggtggg taggcgagcg tctgcaacgt
     2821 tgaggatgat gcgggctaga ggaaaactgg tgcacgaata gggagagtcc ggagcgggca
     2881 actgatcctt cgctacgctc aggatgacaa aagcaacttt gggcgcgcct atccagcaat
     2941 catacattcg cagctacaaa cagaaagaga gatattaccc accatgcaga cccgctatga
     3001 ctccaggacg ctgatttgca tcggcctcac gctcgtgctg tgggcgtcgg ggttcgcggg
     3061 catccgcgcg gggctttcgg gctacgaccc ggctacgatg gccctgctgc gcttcgccgt
     3121 gg
//
LOCUS       DASRWZ010000792         3127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_749257, whole genome shotgun sequence.
ACCESSION   DASRWZ010000792 DASRWZ010000000
VERSION     DASRWZ010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749257"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFH60_12585"
     CDS             complement(<1..786)
                     /locus_tag="VFH60_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314663.1"
                     /translation="MMPSAVRNLIRVFALLGGIGLVVFGMVSGALPGKPKEPPSDGVW
                     LICLGGAFLLFLIYAWLTSTPLVGRYATMDARFRHNVQQLGAFLLVGFVLLSLHLLRE
                     QIVTAVAIKEATVITEEGRVIQDPRKIPEELRTQRGAIMAGDTVVAASEIISPSGFAY
                     RTYPEPNISYLVGYYNPMIYGSAGLEASFDKYLSGSETLNPLTEQQRRLLHRPVIGND
                     LFLTIDPTLQNVAQEQLGERKGSVVVLDTQSGAILAMASWPHYD"
     gene            complement(783..2225)
                     /locus_tag="VFH60_12590"
     CDS             complement(783..2225)
                     /locus_tag="VFH60_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET6314664.1"
                     /translation="MSVASPPTMHTPPAPGVPAVQAGPSRAKQFWGTVRSLRWTEFRL
                     LLVPSILSIVGMLMVILVPTGAMQWQWKDLWMSFVFIGLLAGLHLYLNVFKPRADQVL
                     LPIVATIMVLGLVMIQRLEPALVVKFEEFEGIARKQVLWIVVGMFAMWATVALVRDLN
                     WLRRYKYTFAILGIGLVAATLVFGTDAGSGSGVKLWFDFGFFLFQPSELLKVVLVIFL
                     AGYLDDKRELLASPYRLGPVKLPPIPYIAPLLVLWALALVLFVVQRDLGSALLFFGIF
                     LAMLYVASGRLFFVVGGLALFFAAAYTLSAFFASQFTHIARRVSIWLDPWPVGHDEGY
                     QIVQSLYALASGGVLGVGIGFGSAGIIPAVQTDMIISAIGEELGLAGTLGVVALFMVL
                     VYRGYHIAFASRSGYEQLLAVGLTTILGLQAIIIIGGAIKMIPLTGITLPFISYGGSS
                     LITNCIIIGLLLRVSAPRPVRTDTERLGAP"
     gene            complement(2236..3099)
                     /gene="mtnP"
                     /locus_tag="VFH60_12595"
     CDS             complement(2236..3099)
                     /gene="mtnP"
                     /locus_tag="VFH60_12595"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET6314665.1"
                     /translation="MDTGVIGIIGGSGFYDMPGITDVRTEEVDTPFGKTLDPVVLGRI
                     GGREVAFISRHGKGHRVNPTHVPVRANIFALKLLGVTHLISVSAVGSMREELAPEHMV
                     VPDQLFDRTVHRPRTFFEHGLVVHVAFDKPYCSDLRAKLVAAATQAGGTVHDGGTDMC
                     VEGPQFSTRAESETYRGWGMSIIGMTALPEARLAREAEMCYATLAMVTDYDTWHPDHD
                     SVTVDMVVQTLNKNVHLARNTVAAVISTIGASRECSCGSALQSAIMTAPEMVPADVKQ
                     QMRPLIGRYVE"
BASE COUNT          617 a         1017 c          972 g          521 t
ORIGIN      
        1 gtcgtagtgc ggccacgagg ccatcgcgag gatagccccg ctctgcgtat cgagcaccac
       61 caccgagcct ttgcgctcgc caagctgctc ctgcgccacg ttttgcaggg ttgggtctat
      121 ggtgaggaag aggtcgttgc ctatcacggg gcggtgcagg agtctgcgct gctgctcggt
      181 cagcgggttg agcgtctcgc tgcccgacag gtacttgtcg aacgaagctt ccagccccgc
      241 cgagccgtag atcatggggt tgtagtagcc caccaggtac gagatgttag gctcagggta
      301 ggtgcggtac gcgaagccgg agggcgagat aatctcggag gccgcgacta cagtgtcccc
      361 cgccatgata gccccgcgct gcgtcctcag ctcctccggt atcttgcggg ggtcctgtat
      421 gaccctgccc tcctcggtga tgacggtagc ctctttgata gccacagcgg tcacgatctg
      481 ctcgcgcaac aggtgcaggc tcagcaagac gaaacccacc agcagaaagg ccccaagctg
      541 ctgcacgttg tgccggaagc gggcgtccat agtggcgtac ctgcccacga ggggcgtgga
      601 ggtgagccac gcgtagatca ggaacagcag gaacgcccca cccaggcaga tgagccacac
      661 gccgtcactg ggaggctcct tgggcttgcc cggcagcgcg ccgctcacca tgccgaacac
      721 caccagccca atgccgccga gcagcgcgaa cacgcggatc aggttgcgca ccgcgctggg
      781 catcaaggcg cgcccagcct ctccgtgtcg gtgcgcaccg ggcgaggggc cgacacccgc
      841 agcagcaggc cgatgattat gcagttggtg atgagcgagg agccgccgta gctgatgaag
      901 ggcagtgtaa tgcccgtgag gggtatcatc ttgatcgccc cgccgatgat gatgatggcc
      961 tgcaacccca gtatcgttgt gaggcccacc gccagcaatt gctcgtaacc gctgcgcgag
     1021 gcgaaagcga tatgatagcc ccggtagacc agcaccatga acagggccac cacgcccagc
     1081 gtgcccgcaa gccctagctc ctcaccgatg gccgagatta tcatgtcggt ctgcacggcg
     1141 gggatgatgc cggcggagcc gaagccgatg cccacgccca gcacgccccc cgaagccagg
     1201 gcgtagagcg actgcacgat ctggtagccc tcgtcgtgcc ctacgggcca ggggtcgagc
     1261 cagatgctca cgcgccgcgc gatgtgggtg aactgcgagg cgaagaaggc gctcaatgtg
     1321 taggccgccg cgaagaagag ggccagcccg cccaccacga agaagagccg cccgcttgcc
     1381 acgtatagca tcgcgaggaa gatgccgaag aagagcagcg cgctcccgag gtcgcgttgg
     1441 accacgaaca gcaccagcgc caacgcccaa agcaccagca acggcgcgat atagggtatc
     1501 ggggggagtt tcaccgggcc tagcctgtag ggagacgcca gcagctcgcg cttgtcgtcc
     1561 aggtagcccg ccaggaagat gacgagcacc accttgagca actcggacgg ctggaagagg
     1621 aaaaagccga agtcaaacca caacttgacc ccgctgccgc tcccagcgtc ggtgccgaac
     1681 accagtgttg cggccacaag cccaataccc aatattgcga aggtgtattt gtagcgccgc
     1741 agccagttga ggtcgcgcac cagggccacc gtagcccaca ttgcgaacat ccccaccact
     1801 atccaaagta cctgcttgcg cgcgatgccc tcgaattctt cgaatttgac caccagggca
     1861 ggctccaggc gctgtatcat cacgaggccg agcaccatga tggttgccac tattggcagt
     1921 agcacctggt cggccctggg cttgaatacg ttgaggtaga ggtgcaagcc cgccagcaag
     1981 ccaatgaaca cgaagctcat ccagaggtct ttccactgcc actgcatcgc gcccgtgggc
     2041 accaggatga ccatgagcat gcccacaatg ctgagtatcg aggggaccag gagcaggcgg
     2101 aactcggtcc agcgcaggga gcgcaccgtg ccccagaact gtttcgcgcg agaaggcccg
     2161 gcctgtacgg cgggcactcc cggcgcgggg ggtgtatgca tggtgggtgg agatgctacg
     2221 ctcataagcc gctaactact caacgtacct gcctatcagc ggtctcatct gctgctttac
     2281 atcggcaggg accatctcag gcgcagtcat gatagcactc tggagggcgc taccacaact
     2341 gcactcgcgg ctagccccga ttgtggaaat caccgcggcc accgtattgc gggccaggtg
     2401 cacgttcttg ttgagcgtct gcactaccat atcgaccgtg acgctgtcgt ggtcggggtg
     2461 ccaggtgtcg taatcggtga ccatagccaa cgtggcgtag cacatctcgg cctcccgtgc
     2521 cagcctcgcc tccggcaggg ccgtcatgcc gatgatcgac atcccccagc cccggtacgt
     2581 ctccgactcg gcgcgggtgg agaactgcgg cccctccaca cacatatctg tgccgccatc
     2641 gtgtacggtt ccgcctgcct gggtcgcggc tgctaccagc ttcgcccgca ggtcggagca
     2701 gtagggcttg tcgaacgcca cgtggaccac caggccgtgc tcgaagaagg tgcgggggcg
     2761 atgcaccgtg cggtcgaata gctggtcggg caccaccatg tgctctgggg caagctcctc
     2821 gcgcatgctg cccaccgcgc tcaccgagat aaggtgcgtc acccccagca gcttgagggc
     2881 gaagatgttg gcgcgcaccg gcacgtgggt ggggttgacc ctgtgcccct tgccgtgccg
     2941 cgatataaag gccacctcgc gcccgccaat cctgcccagc accaccggat cgagggtctt
     3001 accgaacggc gtgtccacct cctcggtgcg cacgtccgta attcccggca tgtcgtaaaa
     3061 ccccgaccct ccgatgatgc cgatgacccc tgtgtccatg ctacccccag tcgattttag
     3121 attttag
//
LOCUS       DASRWZ010000793         4747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_407436, whole genome shotgun sequence.
ACCESSION   DASRWZ010000793 DASRWZ010000000
VERSION     DASRWZ010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407436"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1859
                     /locus_tag="VFH60_12600"
     CDS             126..1859
                     /locus_tag="VFH60_12600"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET6314666.1"
                     /translation="MSKLFSQTLRDAPSDVEFASHKLLLRAGFIRQLAAGIFSYLPLA
                     RRSMSKIEAILREEINGIGGQEVTMPVVHPADIWRETGRWYSIGSELGRFRDKNERDM
                     VLAMTHEEVVGDLVRREVRSYRQLPQLIYHIQTKWRDDPRPRAGLIRVREFTMLDSYS
                     LDADWEGLDAQYRAHYDAYFRIFARCGLLAIAVQSDVGMMGGSLAHEYMYLTPMGEDT
                     LILCDNCGYAANRQIATFRKPQGEEEQARPVEKVATPNATTIEQLARFLGIPASRTAK
                     AVFMVATVSEGAESVEKFVFAVVRGDMDLNETKLANAVKANELRPAREEEIRGVGAEP
                     GYGSPVGVRGAVVVVDEAVAGSPNLVAGANAPGYHLLNVNYGRDYTGDIITDIAAARE
                     GDACVQCGSPLRASGGVEVGNIFKLGTRYTDAMGATFLDRDGVSRPVVMGSYGIGVGR
                     LLACVAEEHHDERGLVWPISVAPYEVHLVSLGGEGTQAQQLAYDLYSRLEAGGVEVLY
                     DDREASPGVKFADADLIGVPLRLTVSARSLERGGVEVKRRAGGEGVIVGVEAVVEYVA
                     GEIRGMRQTEL"
     gene            1951..2244
                     /locus_tag="VFH60_12605"
     CDS             1951..2244
                     /locus_tag="VFH60_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002777575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HET6314667.1"
                     /translation="MLYEWDEEKNRTNLLKHGLDFTDAWEIFNAPMLILLDDREDYGE
                     DRWIGIGLLRSRVVVVIYTERGEDTIRIISLRKALSHERTQYEKALRDGLGQS"
     gene            2207..2485
                     /locus_tag="VFH60_12610"
     CDS             2207..2485
                     /locus_tag="VFH60_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006510088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HET6314668.1"
                     /translation="MKKPSETDWARVDALEDDAIDTSDIPALTDAQFAKMTLRVPTEL
                     TAVTVSVDPEVLAWFRSQGEDFEQRINAALRVYVEANRKSGSHSLHGS"
     gene            complement(2653..3336)
                     /locus_tag="VFH60_12615"
     CDS             complement(2653..3336)
                     /locus_tag="VFH60_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6314669.1"
                     /translation="MDDQTLVREGFRKLLELEPDFEVVGTAGDGESAITAMERLAEAQ
                     TPPDVVLMDIRMPRMNGIEATRRIKQRWPGAHVVILTTFDDTELIQEGLSAGALGYSL
                     KDITAEQLSMTVRLASSGQVLLQPNIASKVFASFSPATPPTPPAPKEPHAPAPPGPTS
                     PDLAEPLTEREREILTLVGKGASNREIAEALYLTEGTVKNHMTNILNKIGVRDRTQAA
                     LKARELGLS"
     gene            complement(3360..4685)
                     /locus_tag="VFH60_12620"
     CDS             complement(3360..4685)
                     /locus_tag="VFH60_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6314670.1"
                     /translation="MASTQLPDQRTEGHSALDEWLITRVGDIAVYSTAVIGYLFSVLS
                     SPELTGTGFFLFTAVNVLYLAAAWLTWSRWRRVERVDWGVASGVGAVAILTVAAGLLM
                     PTGLEFDWLLYFATVGMSIVILPPWVGLAVTVLLYLSVPCVYIVTVGPSESSADWLSL
                     LAGFGFVAAFSYSNRLLVTERERSRKLLRQVEESNRELASAHAQLQAYADEVEELAVA
                     RERTRMARDIHDTLGHYLTILSIQLETIAKLQERDPAQAAVEVAEARRVAAHSMQEVR
                     NAVSALRPTSIATLSLPEALAQLGSEFQRVAGDTELTIDLDTDLPPLSADVQLALYRA
                     AQEALTNVRKHARATKVLLRMRYEDEAIKLVVLDNATRVPQDTPQNGSGGFGLLGLRE
                     RVELLGGSVSYGSVEPSGYRVEVSVPVSRVSTRPGAEPELATWKGGEQL"
BASE COUNT          868 a         1380 c         1595 g          904 t
ORIGIN      
        1 gcttcatgcc ctccccgccc caccttcttt agcttcctta attggctttg atatgtataa
       61 gtgccgcatg acgtgcggtg ctgtttggcg ttgcagtggc acgcggaggt atttgcatta
      121 tgcgcatgag taagttattc agccagacgt tgcgggacgc gccgtctgac gtggagttcg
      181 ccagccacaa gctgctgctg cgggcgggat tcatccggca gctagcagct ggtatcttca
      241 gctatctacc cctggcaaga cgctcgatga gcaagatcga ggcgatacta cgggaggaga
      301 taaacggcat aggcgggcag gaggtcacca tgccggtggt gcaccccgcc gacatctggc
      361 gggagacggg gcgctggtac agcatcggct cggagctggg gcggtttcgc gacaagaacg
      421 agcgggacat ggtgctcgcc atgacgcatg aagaggtggt gggggacctg gtgcgccgcg
      481 aagtgaggtc gtaccggcag ctaccgcagc ttatctacca catacagacc aagtggcgcg
      541 acgacccacg tccgagggcg gggctgatac gcgttcgcga gttcaccatg ctggacagct
      601 acagcctcga cgccgattgg gaggggctgg acgcgcagta ccgcgcccac tacgacgcct
      661 acttccgcat cttcgcgcgg tgcggattgc ttgcgattgc ggtgcagtcg gacgtgggca
      721 tgatgggcgg cagcctggcc cacgagtata tgtacctcac cccgatgggc gaggacaccc
      781 tcatcctgtg cgacaattgc ggctacgcgg ccaaccggca gatagccacg ttccgtaagc
      841 cgcagggcga ggaggagcaa gcgcggccgg tggagaaggt cgccacgccc aacgccacca
      901 caatcgagca gctagcgcgg ttcctgggca tcccggcctc gcggacggcc aaggctgtgt
      961 tcatggtggc gaccgtaagc gagggcgcgg agagcgtgga gaagttcgtg ttcgccgtgg
     1021 tgcggggcga catggacctg aacgagacga agctggcgaa cgcggtgaag gccaacgagc
     1081 tgcgaccggc acgcgaggag gagatacgcg gcgttggcgc ggagcccggc tacgggtcgc
     1141 cggtcggcgt gaggggtgcg gtcgtggtgg tggacgaggc tgtcgcgggg tcgcccaacc
     1201 tggtagcggg ggcaaacgcg ccgggctacc acctgctgaa cgtcaattac gggcgcgact
     1261 acaccggcga tatcatcacc gacatcgcgg cggccaggga gggcgatgcc tgtgtgcagt
     1321 gtggcagccc gctgcgtgcc tctggcggcg tggaggtggg caacatcttc aagctgggca
     1381 cgcgctacac ggacgcgatg ggcgctacct tcctggaccg cgacggcgta tcgaggccgg
     1441 tggtaatggg gtcttacggc atcggggtgg ggcggctgct ggcgtgcgtc gcggaggagc
     1501 accacgacga gcgggggctg gtgtggccta tctcggtggc cccgtatgag gtgcatttgg
     1561 tgtcgctggg cggggagggc acgcaggcgc agcagctagc ctacgacctc tactcgcgcc
     1621 tggaggcggg gggcgtggag gtgctgtacg acgaccggga agcctcgccg ggcgtgaagt
     1681 tcgcggacgc ggacttgata ggcgtgccgt tgcgactgac ggtgagcgcg cggtcgctgg
     1741 agcggggcgg ggtggaggta aagcgccggg ctggcgggga gggggttatc gtgggagtgg
     1801 aggcggtggt ggagtatgtg gcgggggaga ttcgggggat gcggcagacc gagttgtagg
     1861 atgcctatac attcctaagg cttccgtcct catccacccc agccctacaa gtcagttgtc
     1921 ttgcagttat actggggcta tactgctacc atgctttatg agtgggacga ggagaaaaac
     1981 agaactaacc tgctcaagca tggtctcgac tttaccgacg catgggagat cttcaacgca
     2041 cctatgctca ttcttcttga tgatcgagag gattacggag aagaccgttg gattggtata
     2101 gggttattga gaagccgcgt tgtagttgtt atatataccg agcgaggtga agacaccata
     2161 cgtatcatct cactacgaaa ggcacttagc catgagcgca cacagtatga aaaagccctc
     2221 cgagacggac tgggccagag ttgacgccct ggaagacgac gccatagata cctcggacat
     2281 ccccgcgctt acagatgccc agtttgcaaa gatgactctg cgcgtgccca cggagctaac
     2341 ggcagtgact gtgagcgtgg acccagaggt actcgcctgg ttccgctcgc agggagagga
     2401 ctttgagcag cgcataaatg ctgctctgcg tgtctacgtt gaagccaata gaaagtccgg
     2461 ctctcattcc ttgcatggct cgtagagcgt acagttttgg ttcactccct atctggccag
     2521 gtactgcatc ccccgataca tctcaccagc cccttgagct ccatcatcgt gagtacggcg
     2581 ctgaccgtgg gggcggggag gcccctgagg ttggtcaggt ggtctatgtg ctgcgtgggg
     2641 tcgtgccatg cctcagctca accccagctc tcttgccttg agcgcggctt gcgtgcggtc
     2701 gcgcactcct atcttgttca ggatgttggt catgtggttc ttgaccgtgc cctccgtcag
     2761 gtacaacgct tcggctattt cgcggttgga cgcgcctttg cccaccagcg tgagtatctc
     2821 ccgttctcgc tcggtgagag gctcggcgag gtcgggggaa gtggggcctg gaggtgctgg
     2881 ggcgtgtggt tcctttggtg ccggtggcgt tggtggggta gcaggggaaa aggaggcgaa
     2941 caccttgctt gcgatgttcg gctggagcaa cacttgcccg ctgctggcga ggcggacggt
     3001 cattgaaagc tgctcggctg taatgtcttt cagcgagtaa cccaacgctc ccgcgctcaa
     3061 cccttcctgg atcagctccg tatcgtcgaa ggtggtcagg atgaccacgt gcgcgcccgg
     3121 ccagcgttgc ttgatccgcc gcgtggcctc gatgccgttc atgcggggca tgcggatgtc
     3181 catcaggacg acgtcgggtg gcgtctgcgc ttcggccagc cgctccatcg cggtgatagc
     3241 gctctcgcca tcgcccgcgg tgcccacgac ctcgaagtcc ggctccaact cgagtagttt
     3301 gcggaacccc tcgcgcacca gcgtctggtc gtccacgacc aggatgcgta tgcgggtcgt
     3361 tatagctgtt cccctccttt ccaggtagca agctcaggct ctgcgccagg ccgggtgctt
     3421 acccgcgata caggcacaga aacctccacg cgataccccg aaggctcgac gctgccatag
     3481 ctgaccgagc cgccgagcaa ctctactcgc tctcgcaagc ccagcaggcc gaaacccccc
     3541 gacccattct gcggcgtgtc ttgcggcact ctcgtcgcat tgtcgagcac gaccagcttg
     3601 atagcctcgt cttcgtagcg catgcgtagc agcactttcg tagcgcgggc gtgtttgcgg
     3661 acgttggtga gtgcctcctg cgcggcccgg tacagggcca gttggacgtc ggctgagagc
     3721 ggggggaggt ccgtgtcaag gtcgatagtt agctcggtgt cgcccgcgac ccgctgaaac
     3781 tcgctcccaa gctgagcgag ggcttcaggt aggctcagcg tggctatgct ggtggggcgt
     3841 agcgccgaca ccgcgttgcg cacctcctgc attgagtgag cggctacacg tcgcgcctcc
     3901 gctacttcga cggcggcttg cgcggggtct ctctcctgga gcttggcgat tgtctcaagc
     3961 tggatgctca ggatggtaag gtaatggccg agggtatcgt gtatgtcgcg ggccatacgg
     4021 gtgcgctcgc gggccaccgc cagttcctcc acctcatccg cgtaggcctg caactgtgcg
     4081 tgtgccgagg ctagctcgcg gttggactcc tcgacctggc gcaagagttt cctggaacgc
     4141 tcgcgctcgg tcaccagcag gcgattcgag taggagaagg ccgctacaaa accgaagccc
     4201 gcgagcaacg atagccaatc cgcagacgat tcggagggtc ccaccgtcac gatgtagacg
     4261 catgggaccg aaagataaag cagcaccgtg accgcgaggc ccacccacgg cgggagaatg
     4321 acgatagaca tccctaccgt ggcgaagtac agcagccagt cgaactccag cccggtgggc
     4381 atgagcaggc cggcggcgac cgtcagaatg gctaccgcgc ccaccccgga ggcgacgccc
     4441 caatcgacac gttccacacg ccgccaccgc gaccatgtca gccaggcggc ggcaaggtat
     4501 agcacgttta cggcggtaaa gaggaaaaag ccggtaccgg tcagctcagg cgacgatagc
     4561 acgctgaaga ggtagccgat aaccgctgtt gagtagacgg ctatatcgcc caccctggtt
     4621 atgagccatt cgtctaaagc tgaatgcccc tcggtgcgtt gatctggcag ttgtgtactc
     4681 gccacgccgt tccttcccct gagatacctg cccgtttggt gagagtataa catagcgccc
     4741 cgccccc
//
LOCUS       DASRWZ010000794         3667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_680786, whole genome shotgun sequence.
ACCESSION   DASRWZ010000794 DASRWZ010000000
VERSION     DASRWZ010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680786"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..978)
                     /locus_tag="VFH60_12625"
     CDS             complement(313..978)
                     /locus_tag="VFH60_12625"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET6314671.1"
                     /translation="MITASTGSKTWNFILDLLYPPRCGGCDRRGEWLCAGCRESVAPP
                     KAEGHSIMGVHGLVCAGDFDGPLRQAVHNLKYENDTPLALPLAEILHAALEHSPYWPD
                     LQSLAPAIVPVPLHREKERARGYNQSHLLGLELARRTGWVLASNLKRLKHTRSQVGLD
                     AQERAENVRDAFVWEGDGTYPAVLLLDDVFTTGSTLGECVAVVRATGARRIYIATVAR
                     ARG"
     gene            1163..2152
                     /locus_tag="VFH60_12630"
     CDS             1163..2152
                     /locus_tag="VFH60_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6314672.1"
                     /translation="MATVAQAERITAPAQGSQSALIWQRFRRHRAGMTGLLVLVLLSL
                     SVVIIPMVSPFTLYEANGLQQYAPFGTMDLIKHEVHWLGTDTFGRDEMVRLFTAGRVS
                     MFVAFGSALLIVLLGSLIGATAGYYGGWVDSLLMRFTDLMLAIPLLPVYLFALRLLRG
                     ADFLREGWNNPDTNSWLNLGLVMAVFTLFGWMSLARIVRGSVLTLRSLEFVEAARALG
                     ASNRRIIFRHLLPNSVAPIVVAATFAVGDFIILEAILSYFTHGITDPPFPTWGNMLAL
                     FQGLALNITSLNPFENVRGYLFLLPSIMILITVLSINYVGEALRSALDPHRIE"
     gene            2191..2628
                     /locus_tag="VFH60_12635"
     CDS             2191..2628
                     /locus_tag="VFH60_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314673.1"
                     /translation="MLRLEKATGGARISKMPQLANLFAMLLLCASVLYVLLAIAPFYL
                     HGINQRSYAEIFGSAVDVKGYAPFSWPVFGPLFQLGAMLSVFLVRVFCPLVCGALAIS
                     GWLGWQRSAPAGRIAWLVAGTVALSLTLVSWTTQDIILTWLAD"
     gene            2711..>3667
                     /locus_tag="VFH60_12640"
     CDS             2711..>3667
                     /locus_tag="VFH60_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6314674.1"
                     /translation="MTTYIIRRFLQSAIFIVFVWMLVYTVLVLLMPLGPKYKYEHFAG
                     FHFPGEMTDAELRQLYKLDKPWPANFFLWLFDPNDTSKQDPNSIEGVLRKGIDVNVLG
                     LRLQGSGALTGNFGISERVLAGREVGEAIAERWDNTVWLVGVSLVVAVLVAVPLGIIA
                     AMNYRSSIDHMITFLSFAGFSIPPYAFGVILIITLAVVPYYLHNFNGYTWLPYLPPGD
                     VASIGQRDNLLDRVYHLVLPVVTLALPQIAYLSRHMRFAMLEILRLEYIRTAWAKGLS
                     GRRVMFKHAFRNALIPLVTAIGLMVPLLVSGAILVETVFGYPG"
BASE COUNT          637 a         1117 c         1126 g          787 t
ORIGIN      
        1 agttcatctg caagtacagt tgggtgggcc tggacgaggg agtgcggctg tccaggggcc
       61 tgggcgactg gctgcacgcg ttgcgtgacg agtgactata gcgccgcccc gtggccttca
      121 ccctgcatgg acgccctgtg gctaaagcca cgggcttaca agaggcaaag tcccctgcgg
      181 ggactaagca acatcatgca gtctccgaag gagacttcgt cctttgtcag cccgccaatt
      241 tattggcagg cggatgccac agggctattg cagcgtgtct catatcagcg gtaattcgta
      301 attcgtaatt actcaccccc tcgcccgcgc caccgtcgct atgtagatgc gcctggcacc
      361 tgtggctcgt actactgcca cgcactcgcc cagggtggag ccggtggtga atacgtcgtc
      421 cagcagcagg acggcggggt acgtgccgtc cccttcccat acaaaggcgt cacgcacgtt
      481 ctcggcgcgt tcctgggcat cgaggcccac ctgggagcgg gtgtgcttca gccgctttag
      541 gttggaggcc aatacccagc cggtgcgccg ggcaagctcc aggccgagca ggtgcgactg
      601 gttgtagccc ctggcacgct ccttctccct gtgcagcggc accggcacta tggcgggggc
      661 gagactttgc aggtcaggcc agtaggggct atgttccagc gcggcgtgta ggatctcagc
      721 gaggggaaga gcgagcgggg tgtcgttttc gtacttgagg ttgtggacgg cctgccgcag
      781 cggcccatca aagtcgcccg cgcacaccag gccgtggacg cccatgatgc tatgcccttc
      841 ggccttgggc ggggccacgg attcgcggca cccggcgcat aaccactcgc cccgcctatc
      901 gcaccctcca cagcggggcg ggtagagcag gtccagtatg aagttccagg tcttggagcc
      961 tgtagacgct gttatcatgt caccacctcg ccagccacaa cagtttacca ctttcgtcca
     1021 ggtctcttgc cggccccagc cggcgtagcc ctgttacttt tcacgccggg cagccgataa
     1081 tcgtcatagc cgggccgcat atggatactg gtccaaccca gcgcatgtcc acaccacggt
     1141 tactcttctt aggagacagt gtatggctac cgttgcacag gcggaacgga taaccgcgcc
     1201 tgcccagggc agccagtccg cgctcatctg gcagcgcttt cgcaggcacc gggcgggcat
     1261 gaccggtctc ctggtgctgg tgctgttgtc tctctcggtc gtcatcatcc ccatggtctc
     1321 ccccttcacc ctatacgagg ctaacgggct gcaacaatac gcgcccttcg gcacgatgga
     1381 ccttatcaag catgaagtac actggctcgg caccgatacg ttcggcaggg acgagatggt
     1441 gaggctcttc acggcggggc gcgtctcgat gttcgtggcg ttcggctcgg ccctgctgat
     1501 cgtgctgctc ggctccctta tcggggccac cgcgggctac tacggcggtt gggtagacag
     1561 cttgctcatg cgcttcaccg acctgatgct ggctataccg cttctgccgg tgtacctgtt
     1621 cgcgttgcgc ttgctcaggg gagcggactt cttgcgggaa gggtggaaca atcctgacac
     1681 caactcgtgg ctcaacctgg ggttggtcat ggctgtgttc accctgttcg gctggatgag
     1741 cctggcaagg atcgttcgag gctccgtact cacgttgcgc tcgctggagt tcgtggaggc
     1801 ggccagggcg cttggagcca gcaaccgtcg catcatcttc aggcacctgc tgcccaactc
     1861 ggtcgccccc atcgtggtgg ccgctacttt tgccgtgggc gacttcatca tcctcgaagc
     1921 catcctgagc tacttcaccc acggcatcac cgacccgccc ttccccacct ggggcaacat
     1981 gctggccctg ttccaggggc tggccctgaa tatcaccagc ctcaacccgt tcgagaacgt
     2041 caggggctac ctgttcctgc tacccagcat catgatcctt attaccgtgc tctctatcaa
     2101 ctacgtcggc gaggcgctcc gaagcgccct cgacccgcat agaatagaat gagtggccct
     2161 gggctagacc ctcacgggag gtagttcgat atgctgcgat tggaaaaagc cacggggggc
     2221 gctcgaatat cgaagatgcc gcagctcgcc aacctgtttg ccatgctgct gctctgcgcg
     2281 agtgtcctgt acgtcttgct ggcgatagcg cccttctacc tgcacggcat caaccagcgc
     2341 tcttacgcgg agatattcgg ctcggcggtg gacgtgaagg gctacgcgcc gttctcctgg
     2401 cccgtgttcg gcccgctgtt ccagttggga gcgatgctga gcgtcttcct ggtgcgggtc
     2461 ttctgtccgt tggtgtgcgg cgcacttgct atcagcggct ggctgggctg gcagcgcagc
     2521 gccccggcgg gcaggatagc ctggctggtg gcgggcaccg tcgccctctc gcttacgctc
     2581 gtgtcatgga ccacccagga catcattctc acctggctgg ccgattgatg atacctcaag
     2641 ttatgccagg actttctata agcatgggaa ggacaagcga caggcgaata atggagcacg
     2701 gcataatatt atgacaacct acataatcag gcggttcctg caaagcgcaa tcttcatcgt
     2761 gttcgtgtgg atgctcgtct acacggtgct tgtgctgctc atgcccttgg ggcctaaata
     2821 caaatacgag cacttcgccg gatttcactt tccgggggag atgacggacg cggagttgag
     2881 gcagctatac aagctggaca agccctggcc cgctaacttc ttcctgtggc tgttcgaccc
     2941 gaacgatacg agcaagcaag accccaacag tatagagggc gtgctacgta agggtattga
     3001 cgtgaacgtg ttagggttgc ggctgcaagg ctccggggcg ctcaccggca atttcggcat
     3061 ctcggagcgg gtgcttgccg ggcgagaagt gggcgaggcc atcgcggaga ggtgggacaa
     3121 tacggtgtgg ctggtaggcg tatcgctggt ggtcgcggtg ctggtggccg tgcccctggg
     3181 catcatagcg gccatgaact accgcagcag catagaccac atgatcacct tcttgtcctt
     3241 cgcggggttc tcgataccgc cttatgcttt cggggtgata cttataatca cgctggcggt
     3301 ggtgccttat tacctgcaca acttcaacgg ttatacctgg ctgccgtatt tgccgccggg
     3361 cgatgtagcc tcaatcgggc agcgcgataa cctgctcgac cgtgtgtacc atctcgtgct
     3421 gccggtggtg accctggcgc tgccgcagat tgcgtacctt tccaggcaca tgcggttcgc
     3481 gatgctggag atactcaggc tagaatacat aaggacggcg tgggcgaagg ggctatcggg
     3541 ccgccgggtg atgttcaagc acgccttccg caacgccctt atacctctcg ttaccgccat
     3601 agggctgatg gtaccgctgc tggtgtccgg ggccatcctg gtggagacgg tgttcggcta
     3661 ccccggc
//
LOCUS       DASRWZ010000795         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_63957,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000795 DASRWZ010000000
VERSION     DASRWZ010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63957"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..>1557
                     /gene="asnB"
                     /locus_tag="VFH60_12645"
     CDS             50..>1557
                     /gene="asnB"
                     /locus_tag="VFH60_12645"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6314675.1"
                     /translation="MCGILGIWHIDGRPLDLATVRRATTVLRHRGPDDEGYLLVDTAS
                     GRVVLCGGAHTDPELALPRIERFFGEQFNLALGFRRLSILDLSPAGHQPMSSPDGRFW
                     LVFNGEVYNYIELRSELCALGYDFHSGSDTEVVLAAYAEWGPECLKRFNGMWGLAIWD
                     NVKRELFVARDRFGVKPFNTVASEKTFVFASEIKAILAMGVVSFRPSPLAVAGYIAQG
                     RFPSHEKGETFFEGVQSLPGSHYALISQEGRTARRYWRVPSRAEADGGQIEEATRRYG
                     ELFTDAVRLRLRADVAVGTCLSGGLDSSSIVAAGGWLMRHEHAVSLERLGDHQQTFSA
                     VYDTQGPWNERRYIEQVAERTGAGANYVIPTAGRLWADLERLVWHQDEPFQSTSIFAQ
                     WCVMDLARQRGATVLLDGQGADEALGGYRPFAIWLAQLLRSGRFPETVRVMRQIQAAT
                     GLNPAPILARAMVLQLPAPVLARLRGTRLKEAISTSGLRSDLGDRLLARQADT"
BASE COUNT          247 a          471 c          537 g          302 t
ORIGIN      
        1 atcgccgcca gctttgacgc taccctcccg attgcctata attacgtgca tgtgtggcat
       61 cctgggcatc tggcatattg acggtaggcc gctagacctc gccaccgtcc ggcgcgccac
      121 gaccgtgctg cggcacaggg ggccggacga cgagggctat ctgctggtag atacggcttc
      181 cgggcgcgtc gtgctgtgtg gcggcgcgca caccgacccg gagctggcgc tgccccggat
      241 agagcgattc ttcggcgagc agttcaattt agccctgggc tttcgccgcc tctccatcct
      301 tgacctctct cccgccgggc accagccgat gtccagcccc gatggtcgct tctggctggt
      361 gttcaacggc gaagtctaca actacatcga attgcgctca gagctatgcg cgctcggcta
      421 cgattttcat tcgggcagcg ataccgaagt ggtacttgcg gcctatgccg aatgggggcc
      481 ggagtgcctc aaacgcttca acggtatgtg ggggttggct atctgggaca acgtgaagcg
      541 cgagcttttc gtagcccgcg accgcttcgg tgtgaagcct ttcaatactg tggcgagtga
      601 gaagacgttt gtattcgcgt cggagataaa ggctatcctg gctatgggcg tggtgtcttt
      661 ccgcccttcg ccgcttgccg tcgcgggtta catcgcgcag gggcgattcc ccagccacga
      721 gaagggagag accttcttcg agggggtgca atctctcccc ggctcgcact atgcgctcat
      781 ctcccaggag gggcgcacgg cccggcggta ctggcgtgtg ccttcgcgtg cagaggcgga
      841 cggcgggcaa atcgaggagg cgacgcggcg atacggcgaa ctctttaccg atgccgtgcg
      901 gttgcgcctg cgcgcggacg tggctgtggg cacctgcctg tcgggggggc tggactcgtc
      961 gtccatagtg gctgcgggcg gctggctgat gcgccacgag cacgccgtct cgttggagcg
     1021 cctgggcgac caccagcaga cgttcagcgc cgtctacgat acgcaaggcc cctggaacga
     1081 gcgacgttac attgagcagg tagcggagag gaccggggct ggggccaact atgtcatacc
     1141 gacggctgga cggctgtggg cggaccttga gcggctggtg tggcaccagg acgagccgtt
     1201 ccaatctacg agcatcttcg cccagtggtg cgtgatggac ctggcccggc agcgcggggc
     1261 gacggttttg ctggacgggc agggcgctga cgaggcgctt ggcggctaca ggccattcgc
     1321 tatatggctg gcgcaactcc tccgttcggg ccgcttccct gagacggtgc gggtgatgag
     1381 gcagattcag gccgcgacgg gcctcaaccc tgcccccata ttggcgcggg cgatggtgtt
     1441 gcaactgccc gcgcctgtgc tggcacgcct gcgagggacg cggctcaagg aggctatctc
     1501 aacatccggc cttaggagcg acctgggtga caggctgctg gcgcggcagg ccgatac
//
LOCUS       DASRWZ010000796         3612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_133110, whole genome shotgun sequence.
ACCESSION   DASRWZ010000796 DASRWZ010000000
VERSION     DASRWZ010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133110"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VFH60_12650"
     CDS             <1..983
                     /locus_tag="VFH60_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HET6314676.1"
                     /translation="EKRQAFIDKRHNEARIIRDREARGAPDDAEQVISTARSSVDPSA
                     PVPVPPFWGYKVLDQRHIPLDEVFDCLDLKSLFRLSWGAHKLHGAEYERAVETDLMPR
                     LKQMRGDMRRRQILTPKVVYGYFPCQSEGNDVIVYDPDTKEPKIRFTFPRQPDRERLC
                     LADYFASKDSGRMDVIPLQVVTMGDRATEVFEQMQRDGNYAEGYYIYGLSVALAEALA
                     EWTHQLVKRELGLPDWQGRRYSWGYPACPDPAEQVKLLEVLPSDKTIGVGLTEGYVLV
                     PEQSTAALVVHHPQAKYYTTRPLNKGARAGAVDEEEAGRGYVEPGADDAA"
     gene            complement(1114..2907)
                     /locus_tag="VFH60_12655"
     CDS             complement(1114..2907)
                     /locus_tag="VFH60_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314677.1"
                     /translation="MSQTGTTTVQKSPGAPAPQTLRAFVARHWFGLYASLTFMGYVVL
                     FLLLQSTILWQIYSPVQARFPMLFGWLSAPFPDPQQRTDADIGWGLAGLLLYMLVLAC
                     LFAAYIFALRRANRLSLSAWASRRLFRRLLAVTAGLLALLMLTREIYAVDVFAYSWFG
                     RIGGVFGDNPYIHVPSDYAASDTGGWLPYLYWHDLPAPYGPAWLILAGAVAKVANVFG
                     SDIAYHVIGHKLLVSAAYLLSVWLIWKVAGQLAGKWVGSAAFAGRASRSRRVGGTQMV
                     PAARKARGLQFAAALAFAWNPLMLIEFGISAHNDLLMLTCVLLALHLHLRGRWQLAVL
                     AVALACLIKFTAIIFLPGYLWLLLSQARRGSPGGSWARALVRVGGAAGIFAATCVAFY
                     VPFWQGPTTLNVLYEDPTAQFFVHSFGTIVNRKLPDLLSALGFASEGLRSREGLEALA
                     STLARWVPLVVTGLVAVWQTWPARNTQRMLQAWGWTIFVLLTVGLAWFWPWYVAWLLV
                     PALLSRDRLLINATILLSFTSLTIYLVGVPLRSIWPDVRLWTALWVMGLPLLYVGYMY
                     RRARAASRAPLQSPATAERATSQAAEGPSHS"
     gene            3034..>3612
                     /gene="dnaX"
                     /locus_tag="VFH60_12660"
     CDS             3034..>3612
                     /gene="dnaX"
                     /locus_tag="VFH60_12660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET6314678.1"
                     /translation="MAASTSLYRKYRSLTFDELVGQEPVVRTLKNAIAAGRIAHAYLF
                     TGPRGVGKTSAARLLARAANCTADAADRPCNRCENCLAVAKDIVTDLIEIDAASNTGV
                     DNIREIIERANFAPSVWHTKFYIIDEVHMLSVSAFNALLKTLEEPPPQTAFILATTEV
                     HKVPATVASRCQRFDFRKIPLSAMSARLRHVCG"
BASE COUNT          710 a         1127 c         1218 g          557 t
ORIGIN      
        1 aggagaagcg gcaggctttc atcgacaagc ggcacaacga ggcccgcatc atacgcgacc
       61 gcgaggccag gggtgcgccc gacgacgccg agcaggtgat atcgaccgcc cgctccagtg
      121 ttgaccctag cgcgcccgtg ccggtgcctc ccttctgggg ctacaaggtg ctcgaccagc
      181 gacacatccc gttggatgaa gtgttcgatt gcctggacct gaaatcgctg ttccggctgt
      241 cgtggggcgc gcacaagctg catggcgcgg agtacgagcg ggcggtggag accgacctca
      301 tgccgcggct gaagcagatg cggggtgaca tgcgccgcag gcagatactg acgcccaagg
      361 tggtgtacgg ctacttccca tgccagtcgg agggcaacga cgttatcgtg tacgacccgg
      421 acacgaaaga gccaaagatt cgcttcacgt tcccgcgcca gccggaccgc gagcgcctgt
      481 gcctggccga ctacttcgcc tcgaaagact cgggccgcat ggacgtgata ccgctacagg
      541 tggtcacgat gggcgaccgg gcgaccgagg tgttcgagca gatgcagcgc gacggcaact
      601 acgcggaggg ctattacatc tacgggctga gcgtggcgct ggcggaggcg ttggcggagt
      661 ggacccacca gcttgtgaag cgggagcttg ggctgcccga ctggcagggg cggcgctact
      721 cgtggggcta cccggcctgc cccgaccccg ccgagcaggt gaagctgctg gaggtgctgc
      781 cgtccgacaa gacgataggg gtggggctga ccgagggcta cgtgctggtg ccggagcaat
      841 cgaccgccgc gctggtggtg catcacccgc aggccaagta ctacaccacg cggccgttga
      901 acaaaggcgc aagggcgggc gcggtggacg aagaggaagc gggccggggc tacgtagagc
      961 cgggtgcgga cgacgcggcc tagctcctta cgccttgcat gggaacaata gggcctattt
     1021 gcaaagtgcc gcccgtgcag taccaagtcc cgcctttgtc cgctcgtttc tacagtttgc
     1081 agatagaccc taatctcgca tgaaggctct agcctacgaa tggctggggc cttctgccgc
     1141 ttgcgaagtt gcgcgttcgg cggtagcggg gctttggagg ggcgcgcggc tggcggccct
     1201 tgcccgtcga tacatatagc ccacgtacag gagcggcagg cccatgaccc agagcgcggt
     1261 ccacagccgc acgtcgggcc agatggagcg gaggggtacg ccgaccaggt agatggtcag
     1321 gctggtgaag ctgaggagta tggtggcgtt aatgagcagc ctgtccctgg ataggagcgc
     1381 gggcaccagc agccaggcca cgtaccaggg ccagaaccag gcaaggccca cggtgagcag
     1441 cacgaaaatc gtccagcccc acgcttgtag catccgttgc gtgttcctcg cgggccacgt
     1501 ctgccacacc gccacaagcc ctgtgacgac caggggcacc caccgggcga gggtagaggc
     1561 aagcgcctcc agcccctccc gcgagcgcag gccttccgac gcaaacccca gggctgaaag
     1621 caggtcgggg agcttgcggt tcacaattgt accgaacgag tgcacgaaga attgggccgt
     1681 cgggtcctcg taaagcacgt tgagagtggt tggcccctgc cagaacggga cgtagaaggc
     1741 cacgcacgtc gcggcgaaaa tgcctgccgc cccacccacc ctgacgagcg ccctggccca
     1801 ggagccgccg ggactgcccc gtctcgcctg cgacagcaac agccacaggt aaccgggtag
     1861 gaagatgata gcggtgaact tgattaggca cgccagcgcc accgccagca ccgccagttg
     1921 ccagcgaccc cgtaggtgca ggtgcagcgc gagtagcacg cacgttagca tcagcaggtc
     1981 gttgtgcgcg ctgatgccga actcgatgag catgagcggg ttccaggcga acgccagggc
     2041 tgcggcgaac tggaggccac gtgccttgcg ggccgccggt accatctggg tgcccccgac
     2101 ccgtcgcgaa cgactcgccc gtccggcaaa cgccgccgag ccgacccact tccccgcaag
     2161 ctgccctgcg accttccaga ttagccacac cgacagcaag taggcggcgc tcaccagcag
     2221 cttgtgtccg atgacgtggt atgcgatgtc gctgccgaag acgttggcga ccttcgcgac
     2281 cgcgcctgcg aggatgagcc aggcggggcc atacggggcg ggcaggtcgt gccagtagag
     2341 gtagggcagc cagccgccgg tatcactcgc cgcataatcc gagggtacgt ggatgtatgg
     2401 gttgtcaccg aaaaccccgc cgatgcgccc gaaccaggag taggcaaata cgtcaactgc
     2461 gtagatttcc cgcgtcagca tgaggagcgc aaggagacct gccgttaccg cgagcagacg
     2521 ccggaacagc cgcctggagg cccacgcgga gagagacagc cggttggccc tgcggagcgc
     2581 gaagatgtac gcggcgaaca agcacgccag caccagcatg tagagcagca gtccggcgag
     2641 gccccaacct atatcggcgt cggtgcgttg ttgcgggtcg ggaaagggag cggagagcca
     2701 accgaacagc atggggaacc gggcctgcac gggggagtag atttgccaga ggatggtgga
     2761 ctgtaggagc aggaacagca cgacatatcc catgaaggtg aggctcgcat ataggccgaa
     2821 ccagtgcctc gcgacaaagg cccggagcgt ttgcggggcg ggcgctcctg gggatttttg
     2881 cacagttgtg gtgccggtct gcgacatggt attagcataa catatgggga attatgcact
     2941 tcttgcgagc gcggcgggcg agtcgtggaa agtcggcaaa tgggctataa tatgcgtgcg
     3001 gcacgaccat cgtccgctgc cgctgaggag tttatggctg cttctacttc cctctatcgc
     3061 aagtaccgct ctctgacgtt tgacgaactg gtgggacagg agccggtggt gcgcacgctc
     3121 aagaacgcta tcgcggccgg gcgcatcgct catgcctacc tgttcacggg gccgcgtggg
     3181 gtcggcaaaa cgagcgccgc ccggctgctg gcgcgcgccg ccaactgcac ggcggacgcc
     3241 gcggaccgtc cctgcaaccg ttgcgagaac tgcctggcgg tcgccaagga tatcgtgacc
     3301 gacctgatcg agatagacgc ggcctctaac acgggcgtgg acaacatccg ggagattata
     3361 gagcgcgcca acttcgcacc ctccgtgtgg cacaccaagt tctacataat cgacgaggtc
     3421 cacatgctga gcgtgtcggc cttcaacgcg ctcctgaaga ccctcgaaga gccgccgccg
     3481 cagaccgcct tcatcctcgc caccaccgag gtgcacaagg tcccggccac cgtggcctcg
     3541 cgctgccagc ggttcgattt ccgcaagata ccgctgagcg ccatgagcgc ccgcctgcgc
     3601 cacgtctgcg gc
//
LOCUS       DASRWZ010000797         7135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_408171, whole genome shotgun sequence.
ACCESSION   DASRWZ010000797 DASRWZ010000000
VERSION     DASRWZ010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408171"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1093)
                     /locus_tag="VFH60_12665"
     CDS             complement(131..1093)
                     /locus_tag="VFH60_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6314679.1"
                     /translation="MRRIAIPPPLAAVLLAVVLFLLSGLLPNGFGSNPDIAGSQATNI
                     IRLAVFLGVVAAGQTLVIISGSEGIDLSAGAVVTLSAIVTYSIANGNNGAVLPALAAS
                     LVVGAVIGLVNGLGVTYLRIAPLVMTLSMAGVVTGLIIVITQGSVTGAVAPVMTQVIA
                     RPVVLGIPGAILIWLVFGALMWLLLQRTTFGKNLFAIGTNRITSRLSGVNVTGMVLAT
                     YSLAGMLAALAGFLVVGNTGVVHIRIGDPFLFPSIAAVAVGGTLLTGGKGSYWGTMAG
                     ALVLTLITSLLTTMQMPESVRRMVLGGTLLIMISIYGRQRGFRQ"
     gene            complement(1090..2058)
                     /locus_tag="VFH60_12670"
     CDS             complement(1090..2058)
                     /locus_tag="VFH60_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6314680.1"
                     /translation="MMNRAWRRLVSQNRLPFALFLLAATIVVNIILQPNFLEPGTLNG
                     SMRVFLPLILVAVGQSIVILGGGIDISAGAIVSIVNTILATQGGVRGEPGTAALMMVL
                     VLFVGMAAGAINGFFVAILRLQPIITTYATSFLFGGLALLILPSPGGGMPSQFTSFYR
                     ETMPVGLPLAFFVIAIIMLAWYGVRQTRYGRYLFAVGGNPDAAYQTGVPVNTVQFSTY
                     VVSGLMSAFAGIALSLLTGSGNAGIGDSLTLAAITAVVLGGTALSGGVGGIGGPIIGA
                     ITLGLLPNIISFSRIETWWQTFLNAAIIVLALATPGVINLFRRRRT"
     gene            complement(2055..3575)
                     /locus_tag="VFH60_12675"
     CDS             complement(2055..3575)
                     /locus_tag="VFH60_12675"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6314681.1"
                     /translation="MTISLSARNVAKRYGAVVALSDANLDIQSGEVLALIGANGSGKS
                     TLSKIINGVVVLDGGQLLVDGSPAHFQSPHAAKRLGISTVFQELSLVPQMSVEENIWL
                     TREPLLGAGIVDRKAVRRKTEELLALFSGMFKTELTPDMPVGPLPPDEKQIIEILKAV
                     SFDPRLLILDEATASLDSRQVQRLFELVTRWKAEGKAIVFVSHRMEEIFQIADRYTVL
                     RNGQTVGEGSMKEVTTRDLVGMMVAGITAENVVQRARPSAEELATRTVPLEARDLHAE
                     GVHGVSFQLHEGELLGLGGLRGQGQHQLLLALFGDIPHSGTATIDGTPVRFTHPRQAM
                     EKRLALVPGERAKEGLLLIRSIFENLLVPSWRRYGFPHRLERARLAASGVSVALNLKM
                     AGLDAPVSSLSGGNAQKVVIGKWLLRNPRVLMLDDPTKGIDVGAKAEFYKLLAQLCDE
                     GKSVLLYSSDDEELIGLSDRVLVMHDGKIRSELIGESLTKANLIAASLGAAEEATA"
     gene            complement(3580..4725)
                     /locus_tag="VFH60_12680"
     CDS             complement(3580..4725)
                     /locus_tag="VFH60_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6314682.1"
                     /translation="MKRKPQVLLSLLIGLPMLLSACGGDTPPAATSTTGTGGAQPTTG
                     TESGGTTPQAAGEKFTIGISNPFISSEYRTQMIQELIDTNKEYMDQGITNELVIESAD
                     TDVAGQLQQLQNLINKDVDAILVNPGDVNALNDTLQEAITKGIIVISVDQELDSPNVY
                     NVGIDQKEWAKTSAKWLAEKMGGKGNIVLIEGFPGHPANSARMSGVDEVLKEYPDIKV
                     LGKDTGRWDEATGQQVMSNFLASFPNMDGYWTQDGMAIGAFQAVLAANPSKFPVPVLE
                     GRCQALQLWDQRLKEDPNFESIAVANPPGVAPTGLRIAVNMLQGKRLDQSKLGGANGQ
                     SFVIPVPVTVTKDNFAEVYKSQCEGKPAAYLLDGIMSDEEVQQYFAK"
     gene            5100..6308
                     /locus_tag="VFH60_12685"
     CDS             5100..6308
                     /locus_tag="VFH60_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET6314683.1"
                     /translation="MQVTEQEAQVLEALRRQAAVSRTDISRITNWSRPKVTGIVNRMI
                     DRGVLMEMGEGDSQGGRRPQLLRLNNRLGYVVGLDIGATSMDLALADLNAEVLSRDRG
                     PADVRVEPDVLLGEVKRRLLDMLRVEGVRPEQVLGVGAGVPGPVDFAKGVLVAPPLMP
                     AWGNYPIRNFFRDLFPNAFVSVDNDVNMMALGELRAGAGKGIDNFIFVKIGTGIGAGI
                     ICHGLVHRGSAGSAGDIGHISVARSGPVCHCGNIGCLEMLAAGPAIADRAAEAARKGT
                     SAILKRKLDANGGRMRAEDVGAAAREGDRSAIEIIQSSGQMVGDVLAGLVNFFNPSLI
                     LIGGGVANIGNQLLASIRQEVLRRSTALATRELVVSYSPMGVEAGTTGAIHLALDHLF
                     VIENTGRVTA"
     gene            6305..>7135
                     /locus_tag="VFH60_12690"
     CDS             6305..>7135
                     /locus_tag="VFH60_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6314684.1"
                     /translation="MTDTTWRVGVIGTGFIGPVHVEALRRLGIEVVALAGNNAERTAE
                     KARQLHIAESYGSVEEMLDRSNIDVVHITSPNHLHVPHARAALFAGKHVVCEKPLAMD
                     SNESRELVRLAKETGRVNAVNFMTRYYPLSQQARSLVRSGELGDVYMVQGSYLQAWLL
                     LPTDWNWRLEPEFSGNTRAIADIGSHWIDLTTYITGLRIESVFADFGTFLTTRQKPKR
                     AVETFTNKAQTPVEYEEQPIYTEDYASVLFRYDSGARGVMTVSQVSAGRKNRMYYEIG
                     G"
BASE COUNT         1318 a         2279 c         2250 g         1288 t
ORIGIN      
        1 tatcctgtta tatgttagtt ttgactttgc atccgcctta tttgcaatgt gcggtctgtt
       61 caaggaatac acgacagaat ttcgcgcact acgatgatgc tgtgtttacc tgacccctat
      121 atagcaaatc ttactgcctg aacccgcgct gccggccgta gatggagatc atgatgagca
      181 gcgtgccccc cagcaccatc cggcgcaccg attccggcat ctgcatcgtg gtgagcaggc
      241 tggtgatgag cgtcagcacc agcgcccccg ccatcgtgcc ccagtagctg cctttgccgc
      301 cggtgagcag cgtgccgccc accgccaccg ccgcgatgga ggggaacagg aacgggtcgc
      361 caatgcggat gtgcaccacg cccgtgttgc ctaccacgag gaaccccgcg agcgccgcca
      421 gcatcccggc gagggagtac gtcgccagca ccatgcccgt cacgttcacg cccgacagcc
      481 gcgaagtgat gcggttggtg cctatcgcga acaggttctt gccgaaggtg gtgcgctgca
      541 acagcagcca catcagcgcg ccgaacacga gccagatcag aatcgcgccg gggatgccca
      601 gcaccacggg gcgggctatc acctgggtca taacgggagc caccgcgccg gtgacgctgc
      661 cctgcgtgat gactataatc aggcccgtta ccacccccgc catgctgaga gtcatcacga
      721 ggggggcgat tcgcaggtac gtaacgccga ggccgtttac aagcccaatt acagcaccca
      781 ccaccaggga ggcggccaat gccgggagca cggccccgtt gttgccgttc gctatcgagt
      841 aggtgacgat agccgacagc gtcaccaccg cccccgccga gaggtcgatg ccctcggacc
      901 cgctgatgat gacgagggtc tgccccgccg ccaccacgcc caggaacacc gccaggcgga
      961 tgatgttggt ggcctgcgac cccgcgatgt cggggttgct gccgaagccg ttgggcagca
     1021 gcccgctcaa caggaacagc acaaccgcca ataggacggc ggccaggggc gggggaatcg
     1081 ctatgcgtct catgtgcgcc tcctgcggaa caggttgatg acaccgggcg tcgccagcgc
     1141 cagcacgata atcgcggcat tgaggaacgt ctgccaccat gtctcgatgc gggagaagga
     1201 gatgatgttg ggaagcaggc ccagcgtgat agccccgatt ataggcccgc ctatgccacc
     1261 caccccgccg ctcaacgccg tgcccccgag taccaccgcc gtgatagcgg ccaacgtcag
     1321 cgagtcgcca atgcccgcgt tgcccgaccc tgtgagcagg ctcagcgcga tgcccgcgaa
     1381 cgccgacatc aggcccgata cgacgtaggt gctgaactgc accgtgttca ccggcacgcc
     1441 ggtctggtag gccgcgtcgg ggttgcctcc taccgcgaac aggtaccgcc cgtagcgcgt
     1501 ctgccgcacg ccgtaccagg ccagcatgat tatcgcgatc acgaagaagg ccagcggcag
     1561 ccccacgggc atcgtctcac ggtagaaaga ggtgaactgc gagggcatgc cgccgccggg
     1621 actgggcagt atcagcagcg ccagcccccc aaacaggaag ctggtcgcat acgtggtgat
     1681 gatcggctgc aagcgcagga tagcgacgaa aaagccgtta atcgcccccg ccgccatgcc
     1741 cacgaacagc accagcacca tcatcaaggc cgccgtgcca ggctcgcccc tcaccccccc
     1801 ctgcgtggcg aggatggtgt tgacgatgga cactatcgcc cccgccgaga tgtcgatgcc
     1861 gccgcccagg atgactatcg actgccccac cgccaccagg atgaggggca ggaacacgcg
     1921 catgctgccg ttgagcgtgc ccggctctag gaagttgggc tgcaagatga tgttgaccac
     1981 gatcgtggcg gccagcaaga acagcgcgaa cggcaggcgg ttctggctga ccagcctgcg
     2041 ccaggcacga ttcatcatgc ggtcgcctcc tccgcagccc ccaggctggc ggcgatcagg
     2101 tttgccttgg tcagcgattc gcctatcagc tccgagcgga tcttgccgtc gtgcatcacc
     2161 agcactcggt cgctcaagcc tataagctct tcgtcgtcgc tgctgtacag gagcaccgac
     2221 ttgccctcgt cgcacaactg ggccagcagc ttgtaaaact ccgccttggc ccccacgtcg
     2281 atgcccttgg tggggtcgtc cagcatgagc acgcgggggt tgcgtagcag ccacttgccg
     2341 atgaccacct tctgcgcgtt gccgccggac aggctgctta ccggggcgtc caaccccgcc
     2401 atcttcaagt tcagggctac cgacacgccg gaggctgcca gccgcgcccg ctccaggcga
     2461 tgggggaagc cgtagcgtcg ccaggatggg acgagcaagt tctcgaatat cgagcggatc
     2521 aagagtagcc cctctttggc ccgctcgcct gggacgaggg cgagcctctt ttccatcgcc
     2581 tgccgggggt gagtgaaacg cacgggcgtg ccgtctatcg tggccgtgcc cgaatggggg
     2641 atgtcaccga agagggcgag caacagttgg tgctggccct gccccctcag accgcccagc
     2701 cccagcaact ccccttcgtg caactggaag ctcacgccgt gcaccccctc tgcgtgcaag
     2761 tccctcgctt ccaacggaac agtgcgtgtg gctagctcct cggcagaggg ccgggcgcgc
     2821 tgcaccacgt tctcagctgt gatcccggcc accatcatgc cgaccaggtc cctggtggtg
     2881 acttctttca tcgagccttc gcccacagtc tgaccgttgc gcagcaccgt gtagcggtcg
     2941 gctatctgga atatctcctc catgcggtgg gagacgaaga ctatagcctt gccctccgct
     3001 ttccagcgcg tgacaagctc gaagaggcgc tgcacctggc ggctgtcgag gctggcggtg
     3061 gcttcatcca gtatcagcag gcgagggtcg aagctgaccg ctttgaggat ctcgatgatt
     3121 tgcttttcgt cgggagggag aggccccacg ggcatgtcgg gggtcagttc cgtcttgaac
     3181 atgccggaga agagggcgag cagttcctcc gtcttgcgcc ggacggcctt gcggtctact
     3241 atgccggcgc cgagcagcgg ctcgcgcgtc agccagatgt tctcctccac gctcatctgg
     3301 ggcaccaggc taagctcctg gaacacggtg gagatgccga gccgcttagc ggcgtggggc
     3361 gattggaagt gggcggggct gccatcaacg agtagttgcc ccccatcgag gacaactact
     3421 ccgttgatga tcttgctcag cgtgctctta ccgcttccat tcgcgcctat cagggccagc
     3481 acctcgccgg actggatatc gaggttggcg tccgagaggg ctaccactgc cccgtagcgc
     3541 ttcgcgacat tgcgggcgga aagcgatatg gtcatggcgt tacttcgcga agtactgctg
     3601 gacttcctcg tcgctcatga tgccgtccaa gaggtaggcg gcgggcttgc cctcgcactg
     3661 gctcttgtag acctccgcga agttgtcctt ggtcacggta acgggcaccg ggattacgaa
     3721 cgactggccg ttcgcgccac cgagcttgct ctggtccaac cgcttgccct ggagcatgtt
     3781 caccgcgatg cgcagccccg tgggcgcgac gcccggcggg ttagctacgg cgatgctctc
     3841 gaagttgggg tcttccttga ggcgctggtc ccacagttgc agggcctggc agcgtccttc
     3901 cagcaccggc acggggaact tggaggggtt ggcggcgagc acggcctgga aagcgccgat
     3961 agccatgccg tcctgggtcc agtagccgtc catgttgggg aaggaagcca ggaagttgga
     4021 catcacctgc tggccggtgg cttcgtccca cctgccggtg tccttgccga gcaccttgat
     4081 gtcgggatat tccttgagca cctcgtccac gccgctcatg cgggccgagt tggcggggtg
     4141 gccggggaag ccctctatga gcacgatatt gcccttgccg cccatcttct cggcaagcca
     4201 cttggccgag gtcttggccc attccttctg gtcgatgccc acgttgtaga cgttcgggct
     4261 gtcaagctcc tggtccaccg agatgacgat gatgcccttc gtgatcgcct cttgcaaggt
     4321 gtcgttcagc gcattcacgt cgccggggtt gacgaggatg gcatccacgt ccttgttaat
     4381 caggttctga agctgctgga gctggcctgc tacgtccgta tcggcgctct cgatcaccag
     4441 ctcattggtg ataccctggt ccatgtactc cttgttggtg tcgatcagct cctggatcat
     4501 ctgcgtgcgg tactcgctgc tgatgaaggg gttggagatg ccaatcgtga acttctcgcc
     4561 cgcggcctgg ggagtggtcc cccccgattc ggtgccggtg gtcggctggg ccccgcccgt
     4621 gccggtcgtg gaggtagcgg cggggggagt gtcgccaccg caggcggaaa gcaacatcgg
     4681 cagcccgatt agtaacgaga gtagaacttg cggtttcctt ttcattgata gctccttctg
     4741 gggtcgtgaa ccatggtcca ttcagcgtcg aaagcgactt gcgagagcgt gtgcagccgt
     4801 tgtagcaatt gaggggccac cattacgcaa gggtacaatg aggtaaaacc atcgcaacct
     4861 attgccacat cagcctatat ggcttatact atgcccgcga ctcgccatca cgcaagttga
     4921 aagaatatta ccatacttac caatgaaatg tacatactta agtaagttta tgaagtaagt
     4981 tcgtgagcct gatgtacggc tcctggtgtg cacacgcccg accacggatt ggattcgccg
     5041 gcagaagtcc caggtgatct tatatgaccc ccaagatgac caaaaatgaa gctccatcgt
     5101 tgcaagttac cgagcaggaa gcgcaggtcc tagaggcgtt gcgccggcag gctgccgtct
     5161 cgcgcaccga tatctctcgc atcaccaatt ggtcgcgccc caaggtcacg ggcatcgtca
     5221 acaggatgat agaccggggc gtgttgatgg agatgggcga aggcgactcg cagggtggcc
     5281 gccgcccgca gctgctgcgg ctcaacaacc gcctgggcta cgtggtcggc ctcgacatcg
     5341 gggccacgag tatggacctg gcgctcgcgg acctcaatgc cgaagtgcta tcgcgcgacc
     5401 gcggccccgc cgacgtgcgg gtggaacccg acgtgctgct tggcgaggtg aagcgccgcc
     5461 tgctcgacat gctgcgagtg gagggcgtgc ggccggagca agtgctcggc gtgggtgcgg
     5521 gcgtgcccgg cccggtggac ttcgcgaagg gcgtgctcgt ggctcctccc ctcatgccgg
     5581 cctgggggaa ctaccccatc cgcaacttct tccgagacct ctttcccaac gcctttgtct
     5641 cggtggacaa cgacgtgaac atgatggccc tgggcgagct tcgggcgggc gcgggcaagg
     5701 gcatcgacaa cttcatcttc gtcaagatcg gcacgggcat tggcgcgggc atcatctgcc
     5761 acggccttgt gcaccggggc agcgccggga gcgcgggcga tatagggcac atctccgtgg
     5821 cccgctcagg ccccgtatgc cactgcggca atattggctg cctggaaatg ctggccgctg
     5881 gccccgcgat tgcagataga gcagccgaag ccgcccggaa agggacaagc gccatcctca
     5941 agagaaagct ggatgcgaac ggaggcagga tgcgagccga ggatgttggc gcggccgcgc
     6001 gggaagggga ccgctccgcg attgagatca tacagagtag cgggcagatg gtgggcgacg
     6061 tgctggcggg cctggtcaac ttcttcaatc ccagcctgat cctcattggc ggcggcgtgg
     6121 ccaacatcgg caaccaactg ctcgcctcca tccgccagga ggtactgcgc cgctccaccg
     6181 ccctggcgac ccgcgagctt gtcgtcagct actcacccat gggcgtggaa gccgggacga
     6241 ccggcgcgat tcacctggcc ctggaccacc tgttcgtcat cgaaaacacc gggagggtaa
     6301 cagcgtgaca gatacaacct ggcgagtggg cgtaatcggt accggcttca tcggccccgt
     6361 gcacgtcgag gctttgcgcc gcctgggcat cgaagtggtg gccctggcag ggaacaacgc
     6421 cgagcgcacg gccgagaagg cgcgacagct ccacatcgcg gagtcttacg gctccgttga
     6481 ggagatgctc gatcgctcca acatcgacgt ggtgcacatc acctcgccca accacctcca
     6541 cgtcccccac gccagggccg ccctgttcgc cggtaagcac gtcgtgtgcg agaagcccct
     6601 tgcgatggac tccaacgagt cgcgcgagct ggtgaggtta gccaaagaga ccgggcgcgt
     6661 caacgcagtc aacttcatga cccgctacta cccgctctcc cagcaggccc gctcgctcgt
     6721 gcggagtggg gagttgggcg acgtctacat ggtgcagggt tcctacttgc aagcttggct
     6781 gctgctgccc accgactgga actggcggct cgaaccggag ttcagcggca acacccgcgc
     6841 catagccgac atcggctccc actggatcga cctgaccacc tacatcacgg ggctgcgcat
     6901 cgagtcggtg ttcgcggact ttgggacgtt cctgaccacc cggcagaagc ccaaacgcgc
     6961 ggtggagacc ttcaccaaca aagcccagac gccggtagag tacgaggagc agcccatcta
     7021 caccgaggac tatgcgagcg tgctgttccg gtacgacagc ggggcgaggg gggtaatgac
     7081 ggtgtcgcag gtgagcgcgg ggcgtaagaa ccgcatgtac tacgagatag gcggg
//
LOCUS       DASRWZ010000798         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_681732, whole genome shotgun sequence.
ACCESSION   DASRWZ010000798 DASRWZ010000000
VERSION     DASRWZ010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681732"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="VFH60_12695"
     CDS             <1..1348
                     /locus_tag="VFH60_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314685.1"
                     /translation="ALLIAVSYFMIFNKTPVALATMVEQGDIVDYSQPVHVLLAKSVK
                     ADDIQNNTTVHEVSGTNLIPAATENKYRSRGPNSGELVIYPVGAWKAGVQYRIVIDAR
                     RVQLWGVVGDNPLGDEPVELTFNTAMYTPTPSSTPTETPMPTDTPVPPTETPEPPAPT
                     AIAQASTEVPVAAASNTPEPPAATATTRPNTPVPPTATRPVPTNTKVAPTATLPPPPT
                     NTVAPSNTPVPEPTATSQPTHAPSATPTVSATPTRRSATPAVTATATVQPRVTPGKVT
                     PTVSPISDCLLEPVRGFGAIWRGDSDVRERLGCPTVPEEPVRPAAQQHFQGGYMFWRG
                     DTRVIYVFLQTGRHDQYGTWFEFKDTWVEGEAVPTVEDGTPDGGYVPVRGFGKLWANN
                     PELRQKLGYATEPEASVDAAWQPFVHGRALWTSDRTIRLMYEDGIWQHFNDTYVGE"
     gene            complement(1480..2268)
                     /locus_tag="VFH60_12700"
     CDS             complement(1480..2268)
                     /locus_tag="VFH60_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET6314686.1"
                     /translation="MRIGIVSDVHGNYVALKAVIEHMGSVDALWCLGDLVGYGPQPNE
                     VVAAVSHYPHLCVPGNHDWGTLGRLESRAFNRDARALLEWTREALTDDHVAYLEGLPL
                     TLTVLSSSITLVHASPRDPMWEYLLDLFDAAECFPLFQSRYCFVGHTHVPLIFRDVAG
                     VVKGLVPEAGEPMRLNVRPYRPGENGDQHNPRMIINPGSVGQPRDGDPRSSYMVLTIP
                     DEDTIDGAATLVQYRVEYQVQETQTLMKSLGFPPRMIARLELGI"
     gene            2615..>3726
                     /locus_tag="VFH60_12705"
     CDS             2615..>3726
                     /locus_tag="VFH60_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET6314687.1"
                     /translation="MQPTPTKLGIHQNSPEPPRPSAAEALGGGGRSGQGTPVTEPQRR
                     PYLPHVLGGLLVLAAGWLYVRTLAPSVMPGDYAEFQFCAAILCVPHPTGYPLYILLGK
                     LFTLLPFGDVAYRVNFSSAAYMVGAVGLLFAIAVRLLKLAGLARVWWCAAVGAALFAV
                     APTVWSMSLVARSYALNALLVGSVVYCLVSWRNTGGRGWFFASCTLIGLSMVHHGTTY
                     LLLPAYGLYLLLVEVERLRRGADKRPWLRRGLGVVALLVGFSPMLFLVYRFVFGSPYY
                     WGNPTTWKDFFRLVTGGPFHNQVMAFGSDPGAQLGRVAFGIGELSVQYTAVGIVLGLV
                     GLVALWRLRRAEAGLLALMLLGNFLFAMNYSLVGYL"
BASE COUNT          660 a         1153 c         1217 g          696 t
ORIGIN      
        1 agcactactc atcgcggtgt cctacttcat gatcttcaat aagacacctg tagccctggc
       61 aactatggtg gaacagggcg atatagtaga ctattctcag ccggtacacg tgctactggc
      121 caaatcagtt aaggccgacg atatacagaa caataccacg gtccatgagg tttccggcac
      181 caatttaatt ccggcagcga ccgagaacaa gtacaggtcg aggggtccga acagcggcga
      241 gctggtcatt taccccgttg gcgcctggaa ggcaggcgtc cagtacagga tagtcataga
      301 tgcgcgccgg gtacagttat ggggcgttgt gggcgataat ccgcttggtg atgagccggt
      361 cgagctgacc ttcaacacgg ccatgtacac gcccacgccc agcagtacgc ctaccgaaac
      421 gcctatgccg acggatacgc ccgtgccgcc caccgagacg ccggaaccgc ccgctcctac
      481 agctatagct caggcatcga cggaggtgcc cgtagcggca gcgtcgaaca cgccggaacc
      541 gcccgcggct accgccacca cgaggcccaa cacgcccgtg ccgcctactg cgaccaggcc
      601 cgtgcccacc aatacgaagg ttgcgcccac ggccacgctg cctccgccgc ctactaacac
      661 agtcgcgccg agcaatacgc ccgtgccgga accgacggcc acgagccaac cgacgcacgc
      721 acccagcgcc acgcccaccg tttcggctac gcccacccgc aggagcgcca cgcccgcggt
      781 tacggctacg gctacggtgc agcccagggt cactccgggc aaggtcacgc cgacggtctc
      841 gcctatctcg gattgtctgt tggagccggt gcgcggtttc ggggctatct ggcgcggcga
      901 ctcggacgta cgcgagcgtc tcggctgccc tacggtgcct gaagagccgg tgcgacccgc
      961 cgcacaacag cacttccagg gcggatacat gttctggcgg ggcgacacgc gtgtgatcta
     1021 cgtgttcttg cagaccggcc gccacgacca gtatggcacg tggttcgagt tcaaggatac
     1081 gtgggtagag ggtgaggcgg tgcccacggt cgaggatggg acgcccgacg gcggctacgt
     1141 cccggtgcgc ggcttcggca agctgtgggc caacaacccg gagctacgcc agaagctagg
     1201 ctacgccacc gagccggagg ctagcgtgga cgccgcgtgg caacccttcg tgcacggccg
     1261 cgccctctgg accagcgacc gcaccatccg cctcatgtac gaggacggca tctggcagca
     1321 cttcaacgac acgtacgtgg gagagtaatg agtgatgagg actgaggact gaggactgag
     1381 tagagaaagc aagaagcaac aaagcggctg tggggatcgt gtacccccac agccgcttta
     1441 ctactactca gtcctcagtc ctcatcactc attacttcct cagatgccca gctccaggcg
     1501 ggcaatcata cgcgggggga agccaaggct tttcatgagg gtctgggtct cctgtacctg
     1561 gtactcgact cggtactgga cgagggtggc ggcgccgtcg atagtgtctt cgtcggggat
     1621 ggtgaggacc atgtaggagg agcgggggtc gccgtcgcgg ggttggccga cgctgccggg
     1681 gttgataatc atgcgggggt tgtgctggtc gccgttctcg ccgggccggt aggggcgcac
     1741 gttcagacgc ataggctctc ccgcctccgg caccagccct ttcaccaccc ccgccacgtc
     1801 tcggaagatg agcggcacgt gggtatgtcc cacgaagcag tagcgcgact gaaagagggg
     1861 gaagcattcg gcggcatcga acaggtcgag caggtattcc cacatcgggt cgcgtgggga
     1921 ggcgtgcacg agtgtgatgc tggacgagag cacggtgagc gtcaggggga ggccctccag
     1981 gtaggcaacg tggtcgtcgg tgagggcctc gcgggtccat tcgaggaggg cgcgggcgtc
     2041 gcggttgaat gctctggact cgaggcggcc cagggtgccc cagtcgtggt tgccgggcac
     2101 gcacaggtgc gggtagtgcg acacggcggc cacgacctcg ttgggttgcg ggccatagcc
     2161 aacgaggtcg cccagacacc agagcgcgtc aacgctgccc atgtgctcta tgacggcctt
     2221 cagggcaaca tagttaccgt gtacgtcgct gacaatgccg atgcgcaaag ttgaaaagcg
     2281 cccttctttc aggggttggg tttcggggta tctcctgcca atatatcaca cgtggcgact
     2341 gtggacaatg ggagagcagt cctgggctgt gtgacatggg cacaggggat gctttggtcc
     2401 cccggcggtg catgacgccc tgtggctaaa gccacgggct tacaagaggc aaagtcccct
     2461 tcggggacta agcaaaatga ggggagtctc cgaaggagac ttcgctactt cccagcccgt
     2521 ggctttagcc acagggcacc cgacagggcc tctattcgag gacacaaccg caaacacgac
     2581 ccgcgcgtgc ctgtgatata ttgcttgtgt caccatgcag ccaaccccaa ccaagctcgg
     2641 catacatcag aactcaccag aacccccgcg cccttccgct gccgaggcac tggggggagg
     2701 aggtcggtcg gggcagggca cgccggtgac cgagccacag cgccggccct acctacctca
     2761 tgtgctgggc ggcttgctcg tgctggctgc gggctggctg tacgtgcgga cgcttgcccc
     2821 ctccgtcatg ccgggcgact acgccgagtt ccagttctgc gccgctatcc tgtgcgtgcc
     2881 ccacccgacc ggctacccgc tatatatctt gctgggcaag ctattcacgc tattaccctt
     2941 cggcgacgtg gcctaccggg tgaacttctc ctcggcggcc tacatggtgg gagcggtcgg
     3001 gttgctgttc gccatcgctg tgcgcttact caagctggcg ggccttgcgc gtgtgtggtg
     3061 gtgcgcggcc gtgggtgccg ccctgttcgc cgttgcccct accgtctggt ctatgtcgct
     3121 cgtggcgcgg agctacgccc tcaacgcgct gctggtgggc agcgtcgtct actgtctggt
     3181 tagctggcgc aacacgggcg gaaggggctg gttctttgcc tcctgtaccc tgattggcct
     3241 gagcatggtg caccacggca ccacatactt gctgctgccc gcctacggcc tctacttgct
     3301 gctggtggaa gtggagcgtc tccggcgcgg cgcggataag cggccctggt tgcggcgtgg
     3361 gctgggggtc gtggcgctgc tggtggggtt cagccccatg ctcttcctgg tgtaccgctt
     3421 tgtgttcggc tccccctatt actggggtaa cccgaccacc tggaaagact tcttcagact
     3481 ggtcacagga gggccgttcc acaaccaggt aatggctttc ggctctgatc caggggcgca
     3541 actgggccgc gtagcctttg gcataggcga gctttccgtg cagtacaccg cagtgggtat
     3601 tgtgctgggg ctggttgggc ttgtggcgct gtggcggctc aggcgggcgg aggccgggct
     3661 gctggcgctc atgttgctgg gcaacttcct gttcgccatg aactactcgc tggtaggtta
     3721 tctcta
//
LOCUS       DASRWZ010000799         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_338771, whole genome shotgun sequence.
ACCESSION   DASRWZ010000799 DASRWZ010000000
VERSION     DASRWZ010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338771"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..653)
                     /locus_tag="VFH60_12710"
     CDS             complement(33..653)
                     /locus_tag="VFH60_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314688.1"
                     /translation="MSNRTQLRERLRLELGDTGSVQVWSDDLLDALLVESVGWYSRLW
                     PRQEAIYRDVAEGERTFSILTGTLGITSVECPPGRVLPHEATGPVGAPQSSGFRQSWS
                     LWGETLYLGRGARGDEVGALKLVMQVLLPWPRLDPFEQWPGPVGDERLLVLWSAVEAW
                     AWLDGQDQKRGRPARSGVMVGRYTEELEREVAARRRAATSRVMSSE"
     gene            complement(770..2128)
                     /locus_tag="VFH60_12715"
     CDS             complement(770..2128)
                     /locus_tag="VFH60_12715"
                     /inference="COORDINATES: protein motif:HMM:NF016988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HET6314689.1"
                     /translation="MMGATGVLSTGSQPGEARPFVLSQEDAMRLAGYRAYLDFYNGAQ
                     WEGLPAPNERRMTFNYARVFANKAASYLMGKGVGFAVDAPEGAGEEGRKAAQHAEGLL
                     EACYRRNSLALVDLDTAVDSAVLGDGVFKVTWDAAEGAVSVAAVDPASITCVRAADDY
                     RRLLAVRQSYVVGNGGAGVTLGSAAGMVVEEWTPETLEVWRDGVLSRRVPNPYGFIPY
                     VVFPNLRVPKEPWGQSDLVDLLQVNRDLNERLSVLSHILEVSGNPVAVLENVTDSTGI
                     KVGPGRLWELPKDSRAYLLDLLSGGGVGLHIEYVNLLYRAMHDLAEMPRTSFGDSNGT
                     ARSGVALEIEMQPLLQKLARKRAIWTVALEERSRMALKLYALHGDGVAATVIDAANSY
                     RLRIVWPPVLPSDRSELVSQETALVASGIHSHRRAMDMLGEGDSEVEWSRVLEERKEV
                     SG"
BASE COUNT          445 a          974 c          599 g          475 t
ORIGIN      
        1 ttgcgttttc gtctctctct actcggcact cgctactcgc tactcatcac tcgactagtg
       61 gccgctcgcc gccgagccgc cacctctcgt tccaactcct cagtgtagcg gcctaccatc
      121 acccctgagc gggcggggcg acctcgcttc tggtcctgcc catcgagcca ggcccacgct
      181 tcaaccgccg accacagcac gagcaaacgc tcgtcaccca caggccccgg ccactgctcg
      241 aacgggtcca ggcgtggcca gggcaggagc acttgcataa ccagcttcag cgcccctacc
      301 tcgtcccctc tagccccccg ccccaggtac agggtctcgc cccacaggct ccagctttgc
      361 ctgaagccgc tactttgcgg agcgcccacc ggccccgtcg cctcgtgagg cagcaccctg
      421 ccgggcgggc actccacgct agtaatgccc agcgtgcccg tcagtatgct gaacgtacgc
      481 tccccttccg ccacgtcccg gtagatcgcc tcctgcctcg gccacaacct gctataccac
      541 cccaccgact ccaccagcag cgcatccagc aagtcgtcgc tccacacctg cacgctcccc
      601 gtatccccca gctccagcct caaacgctcc ctcaactgcg tcctattgct catgcctccc
      661 tccatcagtg acgagtggtt agtgcaagtc tcacgaagaa cccagccaaa caatccttaa
      721 tctttgcgcg ttcttggcgt cctttgcgtc tttgcgtttt cgtctcctac taaccactta
      781 cctctttcct ctcctccaac accctgctcc actccacctc agagtcgcct tcgccaagca
      841 tgtccattgc ccggcggtgc gagtggatgc ctgaggccac cagcgccgtc tcctgagaca
      901 ccagttccga gcggtccgag ggcagcaccg gcggccacac tatgcgcagc cggtagctgt
      961 tggccgcgtc aatcacggtc gcggccaccc catctccgtg cagagcgtac agcttgagcg
     1021 ccatccgact ccgctcttcc agcgccaccg tccagatcgc ccgcttgcgc gccagcttct
     1081 gcaacagcgg ctgcatctct atctccagcg ccacccccga acgcgccgtg ccgttgctat
     1141 ccccaaacga agtgcgcggc atctcggcca ggtcgtgcat cgcccggtag agcaggttga
     1201 cgtactctat gtgcagccct accccgcccc ccgacagcaa gtcgagcagg tacgccctcg
     1261 aatccttcgg aagctcccac aaccgccccg gccctacctt gatgcccgtc gagtcggtca
     1321 cgttttccag caccgctacc gggttgcccg acacctccag gatgtgcgac aacaccgaca
     1381 gcctctcgtt caggtctcgg ttcacctgca acaggtcaac caaatcgctc tgcccccacg
     1441 gctccttggg caccctcagg ttggggaaca ccacgtacgg tatgaagccg taagggttgg
     1501 gcacgcgcct gctcagcacg ccatcccgcc atacttccag cgtctccggc gtccattcct
     1561 caaccaccat cccggccgca gagccaagcg tcacccccgc gcccccgtta cccaccacgt
     1621 agctctgccg caccgccagc agccgccggt aatcgtccgc cgcccgcacg cacgtgatac
     1681 tcgccgggtc caccgccgcc accgacaccg caccctcagc cgcgtcccac gtcaccttga
     1741 acacgccgtc gcccagcacc gcgctgtcca ccgccgtgtc caggtccacc agcgccaatg
     1801 agttgcgcct gtaacacgct tcgagcaacc cctcggcatg ttgtgcggcc ttgcgcccct
     1861 cctcccctgc cccttccggg gcatccaccg cgaagccgac ccccttcccc atgaggtacg
     1921 aagccgcctt gttcgcgaac acgcgcgcgt agttgaacgt catgcgccgc tcgttgggcg
     1981 cgggcaagcc ctcccactgc gccccgttgt aaaagtccag gtaggcccgg taccccgcca
     2041 gcctcattgc gtcttcctgc gacaaaacaa aaggccgcgc ctcacccggc tgcgaccccg
     2101 tagataacac ccccgttgcg cccatcataa gcgcaccccc tttgtaatta gaaattatgt
     2161 cctattatcc ttatcccatt ctcattcaca tttgggacac gatccggctg ccaatgccaa
     2221 acacgttttc acagccgtcg ggcatccatg accatctccg cccttgtcat tccgagcgta
     2281 gcgtggaatc aggtggcagt cgtactcaac cgcaatgcag caggcgagtg gcgaggcacc
     2341 ccggtatggg tacggacggt ggagactgta cggatgctct tctgcaagtt ctgtatgcat
     2401 cttcagtaag gcgagtcttg aagctttgga actcatagaa cgatagtggc tcaaccctga
     2461 ctttagcgtg gcacttttga tccgggcatg ata
//
LOCUS       DASRWZ010000800         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_750383, whole genome shotgun sequence.
ACCESSION   DASRWZ010000800 DASRWZ010000000
VERSION     DASRWZ010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750383"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFH60_12720"
     CDS             complement(<1..256)
                     /locus_tag="VFH60_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314690.1"
                     /translation="MLHHAPGLSYRLGLLLAALAVALPLLLAQQVPSTLSQDLGSAPV
                     PNIEGLGQPEQGEHGAFRWSTGPVEMQLQPLGYPTYVRLFV"
     gene            716..2692
                     /gene="ftsH"
                     /locus_tag="VFH60_12725"
     CDS             716..2692
                     /gene="ftsH"
                     /locus_tag="VFH60_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET6314691.1"
                     /translation="MTDNKWLRNSFVWVIIMVAMLVLFFTFLGNRNTSQEISISKVVE
                     DAKGGNIAKIVTHEDSYTITLCSDAECKQPTRTAQKEPDGNLQDYLLRAGVPPEKMPE
                     ITVEKSSSIGNYLGILGFLLPTLVLVAAFVFMMRQAQGTNNQALSFGKSRARLFTGNK
                     PTITFQDVAGVDEAKQELAEVVEFLKYPEKFNALGARIPRGVLLVGPPGTGKTMLARA
                     VAGEAGVPFFSISGSEFVEMFEGVGASRVRDLFDQAKRNSPCIIFVDEIDAVGRHRGA
                     GLGGSHDEREQTLNQILVEMDGFDSQTNIIVVAATNRPDVLDPALLRPGRFDRQVVLD
                     RPDIKGRLAILNVHSRGKPLEKNIQLETLAKQTPGFSGADLENLVNESAILAARRNRK
                     TITMSEMEEAIDRVIAGPERRSRLISDREKAITAYHEVGHALVARMLPNVDPVHKISI
                     IARGMMGGYTRVLPGEDRYMWSKSQFKDNLAFALGGRAAEEIIFQEVTTGASNDIERV
                     TEMARQMVTRYGMSLKLGLVALGRKEELVFLGREISEQRNYSDQIALEIDQEVRALVD
                     EAYATAKEILTTYREKLIEISELLIERETLDAADFEALFEGMPRPEPKLVNPPKLKRP
                     EQPAPPATEQPRPIPGLQGAPGMAFQQEAHDGKN"
     gene            2943..>3456
                     /locus_tag="VFH60_12730"
     CDS             2943..>3456
                     /locus_tag="VFH60_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6314692.1"
                     /translation="MRLKHLTLQGFKTFVPRTDLVYGEGITAIVGPNGSGKSNVADAV
                     RWALGEQSLSVLRAKRTEDLIFAGSSNRQPVGMAEVSLTIDNADRLLPLDFAEVTLTR
                     RAYRSGENEYYINKSRVRLRDVLDVASTLGQAYTVVGQGLIDAALSLRPEERRELFEE
                     AAAIRGYFVQR"
BASE COUNT          689 a         1103 c         1052 g          612 t
ORIGIN      
        1 gcacgaacag ccggacgtag gtggggtacc cgaggggctg caactgcatt tcaacgggtc
       61 cggtgctcca acggaacgcg ccatgctccc cctgctccgg ctgtcccagt ccttcgatgt
      121 tgggcaccgg ggcgctgccg aggtcctggc tcagtgtcga gggtacctgc tgcgccagga
      181 gtaacggtag ggcgaccgca agcgccgcca gcagcaggcc gaggcggtac gaaagcccgg
      241 gggcgtggtg aagcaagctc ctcctagcgg ccatagacat cgagtgtggg ctgtttcccg
      301 gttttcaaag tcatgtcggg tccggtttgc tcggcaagcc tgcgatgtgg ttgcacctgg
      361 ctgaaactgc cgggggccgc gctgcgtaaa tacgagtcat ggcgcaggtg gcgcggccgc
      421 tcggcccatg cgtcatgcta tcacaataac atagccgttt gaagtgcaag ccccaacagg
      481 ggggaccggg actgaagatg caagctcaac ggacaatctg ctttgacacc cctggccccg
      541 cggtagtata atcaagcgtg tggatgtgct tccgtggccc aatatagaac cctttaggtc
      601 ccacgagcgt taatgtattc agagcaccga ctcactcccc atattcatca aggtaccgaa
      661 ctttatcgct tgacatctgc gatggaagcc ccgaactagt cgcgaggatt cagaaatgac
      721 tgacaacaaa tggttgagaa atagctttgt atgggtcatc atcatggtgg ccatgttggt
      781 tttgttcttc acgttccttg gcaaccgcaa caccagccag gagatctcga tcagcaaagt
      841 ggtcgaggac gccaagggcg gtaatatcgc taagatcgtc acccatgagg acagttatac
      901 catcactctg tgctcggatg ccgagtgcaa gcagccaaca cgcacggctc agaaggaacc
      961 cgatggcaac ctacaggact acctgctgag ggccggcgtg ccgcctgaga agatgccgga
     1021 gatcacggta gagaagtctt cctctatcgg caactacctg ggcatcctcg gcttcttgct
     1081 gccgacactc gtgctggtag ccgctttcgt ctttatgatg cggcaagcgc agggcaccaa
     1141 caaccaggcg ctatcgttcg gtaagtctcg cgctcgcctc ttcaccggca acaagccgac
     1201 gatcaccttc caggacgtgg cgggtgtaga cgaggcgaag caggagcttg ccgaagtggt
     1261 ggagttcctc aagtatcctg agaagttcaa cgctctcggc gcgcgcatcc cgcgcggcgt
     1321 gctgctggta ggccctccgg gcaccggcaa gacgatgctc gctcgtgcgg tggcgggaga
     1381 ggctggcgtg ccgttcttct ccatctcagg ctccgagttc gttgagatgt tcgagggcgt
     1441 tggcgccagc cgcgtgcgcg acctgttcga ccaggccaag cgcaactccc cctgcatcat
     1501 cttcgtggac gagattgacg cggtaggtcg ccatcgcggt gccggtctcg gcggctcgca
     1561 cgacgagcgc gagcagacgc tgaaccagat actggtggag atggatggct tcgatagcca
     1621 gaccaacatc atcgtagtcg ccgccaccaa ccgccccgac gtgctcgacc ccgcgctcct
     1681 gcgccccggc cgcttcgacc gccaggtggt gctcgaccgc cccgacatca agggccgcct
     1741 cgccatcctc aacgttcact ctcgcggcaa gccgttggag aagaacatcc agcttgagac
     1801 ccttgccaag cagacgcccg gcttctcagg cgcggacctt gagaacctgg tgaacgagtc
     1861 ggctatcctg gccgcccgcc gcaaccgcaa gacgatcacc atgtccgaga tggaagaggc
     1921 catcgaccgc gtgatagccg gccctgagcg tcgcagccgc ctcatatccg accgcgagaa
     1981 ggccatcacc gcttaccacg aggtcggcca cgcgctggta gcccgcatgc tgcccaacgt
     2041 ggaccccgtg cacaagatca gcatcatcgc acgcggcatg atgggcggct acactcgcgt
     2101 gctgcccggc gaggaccgct acatgtggtc caagtcgcag ttcaaggaca acctggcctt
     2161 cgcgctcggt ggccgcgccg cggaggagat catcttccag gaagtgacca ccggcgccag
     2221 caacgacatc gagcgcgtga ccgagatggc ccgccagatg gtgacccgct acggtatgag
     2281 cctgaagctc ggcctggtgg ccctgggccg caaggaagag ctggtgttcc tgggccgcga
     2341 gatctcggag cagcgcaact actccgacca gatcgcgctc gaaatcgacc aggaagtccg
     2401 cgccctcgtg gacgaggcct acgcgaccgc gaaggagatc ctcaccacct acagggagaa
     2461 gttgatcgaa atcagcgaac tgctcatcga gcgcgaaacg ctggacgccg ccgacttcga
     2521 ggcgctgttc gagggtatgc ctcgacctga gccgaagctg gtcaacccgc ccaagctcaa
     2581 gaggccggag cagcccgccc cgcccgctac cgaacagccc cgcccgatcc ccggtctgca
     2641 aggcgctccc ggtatggcgt ttcagcagga agcgcacgac ggtaagaact gaacgacagg
     2701 gcatacgtga gcagggtaga tacagcaaag gccactcccg cgagtggcct ttgctttaat
     2761 tatcttaggt gtgccgctct gccaaactag ctgccgaagc tttccacttc ccgccgccct
     2821 gtggtacaat caaagggtgc ccgcccggtg actgaccggg accctctatg ccgggcgctg
     2881 gattatcacg tgaatatcag cccctttctc accatgccgg ggccgcgcac tcaccacttc
     2941 ccatgcgtct caaacacctc accctgcaag gattcaagac tttcgtcccc aggaccgacc
     3001 tggtctatgg cgaaggcatc accgccatcg tcgggccaaa cggcagcggc aaaagcaacg
     3061 tggccgacgc cgtccgctgg gcgttgggcg agcagtccct gtccgtgcta cgggccaagc
     3121 ggaccgaaga cctgattttc gccggttcct ccaaccgcca gccggtcggc atggccgaag
     3181 tgtccctcac catagacaac gcggaccgtc tgctgcccct cgatttcgcg gaggtcaccc
     3241 tgacgcgccg ggcgtaccgt tcgggcgaga acgagtacta tatcaacaag agccgcgtgc
     3301 gcctgcgcga cgtgctcgat gtagcttcga cgctggggca ggcgtacacc gtggtgggtc
     3361 aggggttgat tgacgcggcc ctgtcgttgc ggcccgagga acgccgggag ctgttcgagg
     3421 aagccgccgc catacggggt tacttcgtgc agcggg
//
LOCUS       DASRWZ010000801         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_338860, whole genome shotgun sequence.
ACCESSION   DASRWZ010000801 DASRWZ010000000
VERSION     DASRWZ010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338860"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1591
                     /locus_tag="VFH60_12735"
     CDS             86..1591
                     /locus_tag="VFH60_12735"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HET6314693.1"
                     /translation="MSEKMERLKAILAETVALNQVVGLLNWDRETYMPEGAAQARGNQ
                     LGVIAKLQHEMFTSEEVGRLLEDLAAETADLDPDSDDARLVKVTRRDYEQYSRVPTSL
                     VQELQQGTAEGIPAWHHAKEAQDFKLFVPALKRNNELSQQLADALGYEERPYDAFVNI
                     FEPGMSTTQLETIFSELKETIVPMVREIVKRQDAVDDSFLFQHFEPQQQLDFSMGIAK
                     QYGYDNSRGRLDLTAHPFCQSMSRDDVRITTRVLPEFLNACLFAVLHETGHALYEQGV
                     SPDLAFTPLSSGTSAGVHESQSRLWENLVGRSRAFQDYLFPRLQETYPQQLGNVDVER
                     FYRAVNKVYPSYIRVEADEVTYNLHVLLRFELENAMLEGRIDIDTLDEAWNDKMQEYL
                     GIVPSNPVEGVLQDIHWAWGQGYSFPGYTVGNVVGAQFFAQARKDMPDLDEQISRGEF
                     SNLLGWLQTNIYRHGRKFDANELAQRITGEPVNTRAWREYVTSKFPAIYGF"
     gene            1897..3525
                     /gene="purH"
                     /locus_tag="VFH60_12740"
     CDS             1897..3525
                     /gene="purH"
                     /locus_tag="VFH60_12740"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET6314694.1"
                     /translation="MTTPNSRPFNAVVSVSDPAGLEALGQALTGLGATIYATGGTRSK
                     LSAAGVEARSVSELTGFPEILGGRVKTLHPGVLAGVLARRDEEQLAELGEHGLQTIDL
                     VAVNLYPFAKTIEREGVGIAEAVEQIDVGGPTMIRSAAKNFSGVVVLVDPADYEPVLA
                     EWRESGGVGPDTRKRLAAKAFAHVSQYDALIARYLSGGEAGEGSSAGEHRAGDGTAES
                     MPESISLNLRQAQSLRYGENPHQLAALYRDELPVGGPTLVGSLTQLKGPELSYNNLLD
                     ADAALTIVRDYTMPTIAIIKHAGPCGIACGAGEDDLADVFERALASDPVSAFGGIVGV
                     NRPVNDALAEIIARTRFDLLVAPGFSEEAIDLLARRKNLRILIVPDPDVAHRDDLFVS
                     RLAFRQISGGFLVQTRDAGAEQPVMEPATLRHPTLEEIADLTFAWRAVKHVKSNAIVL
                     ARKLATVGIGGGQPSRVDSVKIATGKAGDRARGSVLASDAFFPFPDGVQIAAEAGITA
                     IIQPGGSVNDDAVIEAADEAGLAMVFTHRRHFKH"
BASE COUNT          730 a         1090 c         1206 g          632 t
ORIGIN      
        1 ttgcgttttc gtctctcgtt ccgttttagc acccagcagc ccaaaatggt aaaatggccg
       61 caatcatgac gcgaggagat gtctaatgag tgaaaagatg gagcggctga aggcgatact
      121 ggccgagacg gtggctttga accaggtcgt ggggctgctg aactgggacc gcgagactta
      181 tatgccggag ggcgcggccc aggcgcgggg caaccagctt ggggttatcg ccaagctaca
      241 gcacgagatg tttacgtccg aggaggtggg caggctgctg gaagacctcg cggcggagac
      301 ggctgacctc gaccccgaca gcgacgacgc ccgcctggtg aaggtcaccc ggcgcgacta
      361 cgagcagtat tccagggtgc ccacatcgct ggtgcaggag ttacagcagg ggaccgccga
      421 gggcatcccc gcctggcacc acgccaagga ggcgcaggac ttcaagctct tcgtcccggc
      481 gctcaagcgc aacaacgaac tgagccaaca actggccgac gcgttgggct acgaagaacg
      541 gccttatgac gccttcgtca acatcttcga gccgggcatg agcaccacgc agttggagac
      601 tatcttctcg gagctgaaag agacgatagt gcccatggtg cgcgagatag tcaagcgcca
      661 ggacgcggtg gacgacagct tcctgttcca gcacttcgag ccgcagcagc agcttgactt
      721 cagcatgggt atcgccaaac agtacggcta cgataacagc aggggcaggc tcgatctgac
      781 ggcgcacccc ttctgccagt cgatgagccg cgacgacgtg cgtatcacca cgcgcgtgct
      841 gcccgagttc ctgaacgcct gcctgttcgc ggtgctgcac gagaccggcc acgcgctgta
      901 cgagcaaggt gtcagccccg acctggcgtt tactccgctg tccagcggca cctcggcggg
      961 cgtgcacgag tcgcaatcga ggctgtggga gaacctggtg gggcgcagcc gggcgttcca
     1021 ggactacctc ttcccgcgcc tgcaagagac ctacccgcag cagcttggca acgtggacgt
     1081 ggagcggttc tacagggcgg tcaacaaggt atacccctcg tacatacggg tggaagccga
     1141 cgaagtgacc tataacctgc acgtgctgtt gcgcttcgag cttgagaatg ccatgctgga
     1201 gggccgcatc gacatagaca cgctggacga ggcctggaac gataagatgc aggagtacct
     1261 gggtatcgtg ccctccaacc cggtcgaggg cgtgctacag gacatccatt gggcgtgggg
     1321 ccaggggtat tcgtttcccg gctacacggt cggcaacgtg gtcggcgcgc agttcttcgc
     1381 ccaggcacgt aaagacatgc ccgacctgga cgagcagatt tccaggggcg agttctccaa
     1441 cctgctaggc tggttgcaga ccaacatcta ccggcacggg cgcaagttcg acgccaacga
     1501 gctggcgcaa cgcatcacgg gcgagccggt caacacccgc gcctggcggg agtacgtgac
     1561 gagcaagttc ccggcgatat atgggtttta gctggacgat ggacgccgaa cgatagccca
     1621 caacctatta agtattatct gtgccggagc aggatagcag gaccaagagt ggccctgtca
     1681 tcctgaacgt agtgaaggat ctaaagaagc tggtatcggg ataatgttga atgaaggggt
     1741 aatgttgctg atggttcatc actgagacaa gggcggcatc ttagatcctt cgctacgctc
     1801 aggatgacag ggccattctt gatcgccccg gttgctaggc tgtacgtcct gccccgccat
     1861 tacccatcgt ccattgtccc acaaggagtc tccaccatga cgacccccaa ctcccgacct
     1921 ttcaacgctg ttgtaagcgt ttccgaccct gcggggcttg aggcgcttgg ccaggccctt
     1981 acagggcttg gggccaccat ttacgcgact gggggcacca ggtcgaagct tagcgcggcg
     2041 ggggtggagg cccgttcggt gagcgagctt acgggcttcc cggagatact gggcgggcgc
     2101 gtgaagacgc tgcaccctgg cgtgctggcg ggggtgcttg cccggcggga cgaggagcag
     2161 ctggcggaac tgggcgagca cggcctgcaa acgatagacc tggtggccgt gaacctgtac
     2221 ccgttcgcca agaccatcga gcgggaaggg gtcggcatcg ccgaggcggt ggagcagatc
     2281 gacgtgggcg ggcctaccat gatacgctcg gcggccaaga acttcagcgg cgtggtggtg
     2341 ctcgtggacc cggcggacta cgagccggtg ctggcggagt ggcgcgagag cggcggggta
     2401 ggccccgata cgcgcaagcg gctggcggcc aaggcgttcg cgcacgtgtc gcagtacgac
     2461 gcgctgatag ctcggtacct atcgggtggg gaggcgggag aagggtccag cgcgggcgag
     2521 cacagggcgg gggacggcac tgcggagagc atgccggaaa gcatcagcct caacctgcgg
     2581 caggcccagt cgctgcgcta cggcgagaac ccgcaccagt tggccgcgct ttaccgggac
     2641 gagctaccgg tgggtggccc tacgctcgta ggctcgctca cccagttgaa ggggccggag
     2701 ctttcctaca acaacctgct ggacgcggac gccgccctta ctatcgtgcg cgactacacg
     2761 atgcccacta tcgctatcat caagcacgcg gggccgtgcg gtatagcgtg tggtgccggt
     2821 gaggacgacc tggcggacgt gttcgagcgg gcgctggcct ccgacccggt gtcggccttc
     2881 ggaggtatcg taggcgtgaa caggcccgtc aatgatgcgc tggcggagat aatcgcccgg
     2941 acgcggttcg acctgctggt agcgcccggc ttctcggaag aggctatcga cctgctggcg
     3001 cggcgcaaga acctgcgcat cctcatcgtg cccgacccag acgttgcgca ccgggacgac
     3061 ctgttcgtct ccaggctggc tttccggcag atcagcgggg gcttcctggt gcagacccgc
     3121 gacgccgggg ccgagcagcc tgtgatggag cctgccacgc tgcggcaccc cacgctggag
     3181 gagatagccg acctcacgtt cgcatggagg gccgtcaagc acgtcaagtc gaacgccatt
     3241 gtgctggccc gcaagctggc gaccgtgggc attggcgggg gccaaccgag ccgggtcgat
     3301 tcggtgaaga tagccacagg caaggcaggc gaccgcgcgc gtggctccgt gctcgcctcc
     3361 gacgccttct tccccttccc cgacggtgtg caaatcgccg ccgaggcggg cataaccgcc
     3421 ataatccagc ccggcggctc cgtcaacgac gacgccgtaa tagaagccgc cgacgaggca
     3481 gggttggcga tggtcttcac ccacagacgc cactttaagc attagatttg gaaattagaa
     3541 tttcggattt cgaatttagt aggtatccaa gcgaattcta aatccgaaaa tcgaaatccc
     3601 aaatccaaag tagtagccca tattaaagcc cccattagga ccaatggggg cttttccg
//
LOCUS       DASRWZ010000802         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_202512, whole genome shotgun sequence.
ACCESSION   DASRWZ010000802 DASRWZ010000000
VERSION     DASRWZ010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202512"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..>1660
                     /locus_tag="VFH60_12745"
     CDS             131..>1660
                     /locus_tag="VFH60_12745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314695.1"
                     /translation="MPRYLRPDIRPVFLASFLLVTFLSPTSYAAPMQQAKPKVSAPYL
                     LAGGAGNQGQVSLAGGFMAYSNCKSADCDIYGVELATRREVVITEQGWDEEQPSTDGV
                     RVVWRDARNVDRAYRNSNNQLVNFDLWGTVLGEGKPAPISRAAQMQNRPAVWGNTVVW
                     ADFRDSGGSDDPDAGNIYTTDFPSGREAVLVKASGAQVRPATNGSIVAWADYRNEPDP
                     NGTNADIYGYDLNTGQEFPISTAPDTQTDPAISGNIVVWADWRKGNNTADIYGYDLAT
                     KQEFAISTAPGSQIEPAIWGNIVVWTDWRNEKDPAGSNTDIYGYDLATKQEFSVFVGA
                     APQRAPGVTGGVVAWEDEAKGNKDRDVMGATISGIALTPPPAPPPPLPGTGAHRFPET
                     RRVVTGLFLDYWQNNGGLPQQGYPISEVMREKSDLDGKEYTVQYFERAVFEYHPEQKP
                     PFNVLLSQLGTFRYQQKYPNGAPNQSTNNSPGARFFPETGKTIGGLFLIYWNNHGGLS
                     QQ"
BASE COUNT          330 a          563 c          514 g          253 t
ORIGIN      
        1 ccatgcttgt gctgatcgta accgttagca cgggcacggg cgcaacattg gcctggttat
       61 tgcgacactc tgatgcgcga gcatctcaca aagggagcct cattcctcag aaggtggact
      121 aattctctcc atgccccgat acctgcgtcc tgacataagg cccgtgttcc tggcttcctt
      181 tctccttgta accttcctaa gccccacctc gtatgccgcg cccatgcagc aggctaagcc
      241 gaaggttagc gcgccctatc tgctggcggg tggggcgggc aaccaggggc aggtcagcct
      301 ggccgggggg ttcatggctt actccaactg caagtccgcc gactgcgaca tctacggcgt
      361 ggagctggct acccgccggg aggtggtcat caccgagcag gggtgggacg aggagcagcc
      421 ttccaccgac ggggtgagag tggtgtggcg cgacgcccgc aacgtggacc gcgcctaccg
      481 caacagcaac aaccagctcg tcaacttcga cctgtggggc accgtgctgg gcgagggcaa
      541 gcccgctccc atctccaggg ccgcgcaaat gcagaaccgg cccgccgtct ggggcaacac
      601 ggtcgtgtgg gccgacttcc gagattctgg cggctccgac gaccccgacg cgggcaacat
      661 ctacacgacc gatttcccct ccggcaggga ggctgtgctc gtgaaggcca gtggcgcgca
      721 ggtgcgcccc gccaccaacg gcagcatcgt ggcgtgggcc gactaccgca acgagccgga
      781 cccgaacggc actaacgccg acatctacgg ctacgacctg aatacgggcc aggagttccc
      841 catcagcacc gcgcccgaca cgcagaccga ccccgccatc tccggcaaca tcgtggtgtg
      901 ggccgactgg cgcaagggca acaacaccgc cgacatctac ggctacgacc tggcgacaaa
      961 gcaggagttc gccatcagca cggcccccgg cagccagata gagcctgcca tctggggcaa
     1021 catcgtggtg tggaccgact ggcgcaacga aaaagacccg gcgggcagca acaccgacat
     1081 atacggctac gacctcgcga caaaacagga gttctcggtg ttcgtaggcg cggccccaca
     1141 gcgggcgccg ggggttacgg gcggtgtggt cgcctgggag gacgaagcca agggcaacaa
     1201 ggaccgcgac gttatggggg cgaccatctc aggcatcgcg cttacgccgc ccccggcacc
     1261 accgccgccc ctgccgggca cgggcgcgca tcgattcccg gagacccgcc gggtcgtgac
     1321 gggcctcttc ctcgactact ggcagaacaa cggcggcctc ccccagcagg gctaccctat
     1381 ctcggaggtg atgcgggaga agtctgacct tgatggcaag gagtacacgg tgcagtactt
     1441 cgagcgcgcg gtgttcgagt accaccctga acagaagccg cccttcaacg tgctattgtc
     1501 gcaactgggc acgttcaggt accagcagaa gtacccgaac ggcgcgccca accagtctac
     1561 caacaactcg cccggcgctc gcttcttccc ggagaccggc aagacgatag gcggcctgtt
     1621 cctcatctac tggaacaacc acggcggcct atcgcagcag
//
LOCUS       DASRWZ010000803         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1230879, whole genome shotgun sequence.
ACCESSION   DASRWZ010000803 DASRWZ010000000
VERSION     DASRWZ010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230879"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1700)
                     /locus_tag="VFH60_12750"
     CDS             complement(177..1700)
                     /locus_tag="VFH60_12750"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6314696.1"
                     /translation="MADTQVKADEQAQGVLGRTSWQLLINGQQVDAVSGETFEVTNPA
                     TNQTFARVAKAGREDVDSAVEAAREAFEKGKWVRMGAARRGSLLYKVAQVMRDRYEEI
                     ARLEVTNNGKAMSQAKAELNQAIEDFEFFAGAATKITGNTIPVPGNFLNYTVREPIGV
                     CAQIIPWNYPVMMAAWKLAPALAAGNTVVLKPASATPLTALLLGEICLEAGIPAGVVN
                     VLAGPGAEIGAYLAEHPGVDKVAFTGETETGRAIMRTAAGTLKRVSLELGGKSPNIVF
                     EDADIDHAVNGSLFAIYYSAGQSCEARSRLFVQQGVYDRFVEQFVEKADRLKVGDPFD
                     NSTQVGSLISPAQLERVERYVRLGEEEGGQVLAGGQRASGDGLDKGNFYRPTVLAGVS
                     NKARVSQEEIFGPVVNIMPFATEAEAVQLANDVVFGLAGTLWTKDVGRAHRVAGQIRS
                     GVVTVKTPITPFPGLPFGGYKQSGFGRELSMEALDLYTELKSVLLYIGEKPINPFGI"
     gene            complement(1764..>2256)
                     /locus_tag="VFH60_12755"
     CDS             complement(1764..>2256)
                     /locus_tag="VFH60_12755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET6314697.1"
                     /translation="KLQAAFRKGGTVTAGNSSGINDGASAVLIVSKEAAEEMGLTPMA
                     RIIAGAVAGVDPACMGVGPIPATRKALERAGLTVDDLDLVELNEAFASQSLACIRELR
                     LDPDKVNVNGGAIALGHALGNSGSRLVTTLVHELKRRDGRYGLATMCIGVGQGIAAVI
                     EMV"
BASE COUNT          364 a          747 c          692 g          453 t
ORIGIN      
        1 gatgttgccc ttttcaatgg catccaagta acatagacct ctgcaaagaa aggtggtatt
       61 tgggtgctga aggacgagat tcttcgctgc gctcagaatg acaaaagcgg ggcgtggtat
      121 ttcaaagcct actgtgacac cctcacgcaa ctgcgtcact cgtcaggcgt ccacctttat
      181 ataccaaagg ggttgatggg cttttcgcct atgtaaagga gcacgctctt cagttcggtg
      241 tagaggtcga gagcctccat gcttagctcg cggccgaagc ctgactgctt gtagccgccg
      301 aaaggcaagc ctgggaaggg ggtgatcggg gtcttgacgg tgactacccc ggagcgtatc
      361 tgcccggcga cacggtgcgc gcggcctacg tccttcgtcc agagcgtgcc cgcgagccca
      421 aacactacgt cattggccag ctgcaccgct tcggcttcgg tcgcgaaggg catgatgttc
      481 actacggggc cgaatatctc ctcctgggag actcgcgcct tgttcgacac accggccagc
      541 accgtcggcc tgtagaagtt gcccttgtcg aggccgtcgc ccgaggcgcg ctgcccgcct
      601 gccagcacct ggccgccttc ctcctcgccg agtcgcacgt agcgctccac gcgctcaagc
      661 tgggcggggg agatgaggga gcctacctgc gtgctgttgt cgaacgggtc gcccaccttg
      721 aggcggtcgg ccttctcgac gaactgctcc acgaacctgt cgtagacgcc ctgttgcacg
      781 aacaggcgcg agcgcgcttc gcacgactgc cccgcgctgt agtagatggc gaacagggag
      841 ccgtttaccg cgtggtcgat gtcggcgtcc tcgaagacga tgttgggcga cttgccgcca
      901 agctccaggg ataccctctt gagggtgccg gcggcggtgc gcatgatggc gcggcccgtc
      961 tccgtctctc ccgtgaaggc caccttgtct acgccggggt gctcggccag gtaagccccg
     1021 atctcggcgc ccggcccggc gagcacgttc accacgcccg ctgggatccc tgcttccagg
     1081 caaatctccc ccagtagcag ggccgtgagg ggcgtggcgc tcgcgggctt gaggacgacc
     1141 gtgttgcctg ccgccagcgc gggggccagc ttccaggcgg ccatcatcac cgggtagttc
     1201 cacggtatga tctgcgcgca caccccgata ggctcccgca cggtgtagtt gaggaagttg
     1261 ccgggtaccg ggatggtgtt gcccgttatc ttggtggccg cgcccgcgaa gaactcgaag
     1321 tcctcgatgg cctggttcaa ctcggccttg gcctgagaca tcgccttgcc gttgttggtg
     1381 acttccagcc gggcgatttc ctcatagcgg tcgcgcatga cctgggctac cttgtacagc
     1441 aggctacccc ggcgcgccgc gcccatacgc acccacttgc ccttctcgaa cgcctctcgc
     1501 gcagcctcga ctgccgagtc tacgtcctcg cgcccggctt tggctaccct ggcgaacgtc
     1561 tgatttgtag cggggttcgt gacctcgaac gtctcgcccg aaacggcgtc cacctgctgg
     1621 ccgttgatga gcaactgcca actggtccgc ccgagcacgc cctgcgcctg ctcgtctgcc
     1681 tttacctgtg tgtcagccat cgtgtaatct tactcctcgt tgagtgttgt actgaaggtc
     1741 gtgggtgtga ttatcaccct cgcctagacc atctctatca ctgccgcgat accctggccc
     1801 acgccgatgc acatggtcgc caggccgtac ctgccgtccc gccgcttgag ttcgtgcacc
     1861 agcgtggtga cgaggcggga gccgctgttg ccgagcgcgt ggcccaacgc gattgcgccc
     1921 ccgttcacgt tcaccttgtc ggggtccaga cgtagctctc tgatgcaggc gagcgactgg
     1981 gaagcaaacg cctcgttcaa ctcaaccaga tcgaggtcgt ccacggtaag cccggcgcgt
     2041 tccagtgcct tgcgtgtggc cgggataggg ccgaccccca tgcaggcggg atcgacaccc
     2101 gcgaccgccc cggctatgat gcgggccatc ggcgtaaggc ccatctcttc agccgcctct
     2161 ttgcttacaa tgaggaccgc cgaagcgcca tcgttgatgc cggacgagtt acccgccgtg
     2221 accgtaccgc ccttgcggaa ggccgcctgt agcttc
//
LOCUS       DASRWZ010000804         7390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1299484, whole genome shotgun sequence.
ACCESSION   DASRWZ010000804 DASRWZ010000000
VERSION     DASRWZ010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299484"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1522)
                     /gene="lpdA"
                     /locus_tag="VFH60_12760"
     CDS             complement(83..1522)
                     /gene="lpdA"
                     /locus_tag="VFH60_12760"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET6314698.1"
                     /translation="MSDQQSNTEYDMTIIGAGPGGYVAALYGGLKGLKIAIIEKDRRL
                     GGTCLLRGCIPSKAMVKTGELMLQMSHAEKYGLGLRGEVTVDWEKTVARKDKVVDGLT
                     SGLTGLMKSRKVEVITGHGTITSPRTVSVDGREIRTRNIVIATGSGTVLPTDIQGGDL
                     PGVITSDDILDADLTLKRLPTMPESIIIVGGGVVGMEFASIFNAYGCKVTVVISSARV
                     LRLVDEEVARRFTQVAKGQGIEIITRSDIVKMEEAAGGKIRATIKNGDSGAEEVREVS
                     NVLWAKGRYPYTQNLGLDSIGLRTEQGFIPVDERMMTSVEGVYAIGDVVPGAMLAHVA
                     SYEGEIAVDNITGHYRAADYGAIPDVIFTIPEIASVGMMEQQAKDRGIPVKVTKFPFA
                     ALGRAQAIGEPDGLVKMVCDPTTGIILGMQVMGAHASDVIAECTLAVQQGITAEELAH
                     TIHAHPTMPEAVAETAFAQILGTPLHANR"
     gene            complement(1626..2174)
                     /locus_tag="VFH60_12765"
     CDS             complement(1626..2174)
                     /locus_tag="VFH60_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314699.1"
                     /translation="MVDEAAAIPVLVLTGPVGVGKTAVAGEISNLLAEMGTAHAVIDM
                     DWLRWCHPSPKQDPFHTALGLRNLAAVWANYRAAGAGCLVLVDIVETRDMLAAYHVAI
                     PSANIEVVRLNATLPAILRRLEGRETGASLAWHQARAGELLGLMAEQAVEDLLVDTEG
                     KTVAEVALEVLSAVGWGRKASV"
     gene            complement(2216..3214)
                     /locus_tag="VFH60_12770"
     CDS             complement(2216..3214)
                     /locus_tag="VFH60_12770"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HET6314700.1"
                     /translation="MPATNLLVTPEANCYNEGERPTEAARPFAYMTVSLQPTPTQAPP
                     RVESWRLLAGFDVPGPLNMALDEALLDSVIAGGVPVLRFYTWRPATLSLGTNQALGEI
                     DRAACEAHGFGVVRRLTGGRAVLHQHELTYSVIARENDPRVSGGVVESYRKISAALVE
                     GLRTLGATVSLAAPDKALLRAMSVRRAAVLDEAGEAAEGSSAVCFDAASAYELTAGGK
                     KLVGSAQARRGGALLQHGSILLDIDLDAWVSVFAYASEAGKRRAYTKLPERMTSLRHE
                     VGRAISPEEALAALVSAFERTLSISLERATLERAEEAVALRLAAEKYGSEEWTARR"
     gene            complement(3225..4859)
                     /locus_tag="VFH60_12775"
     CDS             complement(3225..4859)
                     /locus_tag="VFH60_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET6314701.1"
                     /translation="MSQTNHRKTPSSANDRPTPANPRPNPNHQYRRPEPTYRARSVTT
                     GVTKPTQPVAGRPAVARAGTNRGKRVRRITLLVLLALMLVGGGTGYVFYQDTVKPVLD
                     IPGKISKTPVNKRVVDPDGTVSPDATPQLVEVEPPDWASGEPINILLLGLDYRPTEED
                     TRADTQIIVHIDPTAKTATLLSIPRDLYVTIPGFGPGRINQSYQLGDRAWRLDPESLP
                     GGGPTLAMSTISATFGIPIDYYAQVNFQGFEQVVDALGGLKIDVPVPLVDNEYPLSST
                     SYGTSGTPGTYTSSYGATRIYIPAGMQHMNGKTALQYARSRHADSDLGRNQRQQQVLL
                     ALKQQGMNLNILTKLGELANKLSDAVETDMPLSQLPALAGLAQDIGSGSITTCAIDAT
                     MVEQTILDSGADVLMPIWERIRPRVKQCFSDPRLVNENARLSVQNGTTTSGTARIVSE
                     LLAEKGLNVADLSSAADQGQHPRTTITDYTGGQKPHTIEVIKQELGLADTAVLQGDPA
                     DAPIATNFDGMPVDILVMVGDDRLNRQPARMPTRTP"
     gene            complement(4938..6230)
                     /locus_tag="VFH60_12780"
     CDS             complement(4938..6230)
                     /locus_tag="VFH60_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6314702.1"
                     /translation="MSNGDAQTRRDEPLEPLIPRIRVTPRQRARLVLVGIAIFVVWWL
                     LNQSWDALGPFVIALVLAYLMLPLVDRLSRFLPRVVAILAVYAVFIGSVWGLIAWLAP
                     VVGHQVGELAKQAPTYSEQAQGWGRDAMAWYQSLPISDDMRQSIENGLRNSVGAIGSA
                     VQQGVLNTLSAVSRAMGFIVGLLIIPFWLFYTLKDKDRGINAFNNMLPHAWRTDVWRI
                     VRIINGVLSSYIRGQLLLGLIVGVATFIGMLIVGARYPVVLAIISGITEIIPVVGPVL
                     GAVPGLILAAFHPEGWVMVLKVLAVYVLVQQLENNLLVPKIQGDSVKLHPSIIMVALV
                     VGSQVGGLFGLIIAVPVAAILRDVYLYLYRRYSEGYTSREAEASVPSRQDEQTETTQA
                     REQRELAEEEKRPGINSQDELIEKFDDTSEQPAAAAKP"
     gene            6491..>7390
                     /locus_tag="VFH60_12785"
     CDS             6491..>7390
                     /locus_tag="VFH60_12785"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HET6314703.1"
                     /translation="MDEMESGSSGRDDWQYVDGKPTRAGEQVRILPGDPQQARQVGPG
                     SPATQPPGSRRLDPYEPANMDAGRRGAATIAPTGSAVPYQAAQAPPPTIVQVKRGPTA
                     CVILAGTFAVLALSCALLGFATFRGGLDGFGKLDGILPSFSFGMTPTVTIDTQQPAVI
                     SQIRSLSKLETVNYQLETVVTAKSSGPLPDLLTSDKILLVAHGEVVAGIVLSRLKEED
                     VTVVSGTVTVNLPPAEVLYTRLDNDKTYVDDRQTGIFSSPDPNLETELRRVAEREILS
                     AALEDGILTKASENAQTVLRTLIT"
BASE COUNT         1278 a         2467 c         2266 g         1379 t
ORIGIN      
        1 ttggcggttc cgtctctgac tctacagcgc ttatcgccac actggcggtt cggcatgact
       61 cctgtgtatc agcaatgggt acttacctgt tcgcgtgcag gggcgtaccc aggatttgcg
      121 cgaaagcggt ttcggccacg gcttcgggca tagtggggtg ggcgtggatg gtgtgggcca
      181 gctcctcggc ggtgataccc tgctgtacgg ccagggtgca ttcagcgatt acgtccgagg
      241 cgtgcgcgcc catcacctgc atgccgagta tgatgccggt ggtggggtcg cacaccattt
      301 tcaccaggcc gtcgggttcg cctatagcct gggcgcgtcc cagggctgca aaggggaact
      361 tggtaacttt gaccggaatg ccccggtcct tcgcctgctg ctccatcata ccgaccgatg
      421 cgatttcagg gatggtgaag atcacgtcgg gtatggcacc gtagtcggcg gcccggtaat
      481 gccccgtgat gttgtccaca gcaatttcgc cctcgtagga ggctacgtgc gccagcatcg
      541 cgccgggcac cacgtcgcct atcgcgtaca caccctccac cgaggtcatc atgcgctcgt
      601 ccaccgggat gaagccctgc tcggtgcgca ggccgatgct gtcgagcccc aggttctgcg
      661 tgtaggggta gcgtcccttc gcccagagca cgttgctgac ctcgcgcact tcctccgcgc
      721 ccgaatcgcc gttcttgatg gttgcccgta tcttgccccc cgctgcctcc tccatcttga
      781 cgatatccga gcgggtgatg atctctatgc cctggccctt ggcgacctgc gtgaagcgcc
      841 gggctacctc ctcgtccacg agccgtagca ccctggccga gctgattacc accgtcactt
      901 tgcagccgta ggcgttgaat atggaggcga attccatgcc cactacgccg ccgcccacga
      961 tgatgatgct ctcaggcatg gtcggtaggc gcttcaacgt caggtccgcg tccaggatgt
     1021 cgtcggatgt gatgacgccc ggcaggtcgc cgccctgtat atcggtgggc agcacggtgc
     1081 cggagccagt ggcaattacg atgttgcggg tgcgtatctc gcggccatct acagagacgg
     1141 tgcgaggaga ggtaatggtg ccgtgcccgg tgatgacctc caccttgcgc gacttcatca
     1201 gcccggtgag gccgctcgtc aggccgtcca ccaccttgtc tttgcgggcg accgtcttct
     1261 cccagtccac cgtgacttcc ccgcgcaggc ccaggccgta cttttcggca tggctcatct
     1321 gcaacatcag ctcgcccgtc ttcaccatcg ccttggaggg gatgcagccg cgcagcaagc
     1381 aggtgccccc caggcggcgg tccttctcga tgatggcaat cttcagcccc ttgaggcccc
     1441 cgtacagcgc cgccacgtag ccccccggcc ccgcgcctat tatcgtcatg tcgtattctg
     1501 tattactctg ctggtcagac atttttgtac ctctctgaaa aatattgttc ggcgcggacc
     1561 ccctcgcccg caccatgatt aacgttcctg actatctgca tctttacgtg tcggcggagc
     1621 gaacgtcaaa cgctcgcctt gcgcccccac cctaccgcgc tcaacacttc cagcgcaacc
     1681 tcggcaaccg tcttcccctc ggtatccacg agcaagtctt ccaccgcttg ctccgccatt
     1741 agcccaagga gttcccccgc ccgcgcctgg tgccaggcaa ggctagcccc ggtctcgcgc
     1801 ccctccagcc gccgaaggat ggccggtagc gtggcgttca gccttaccac ctcgatgttc
     1861 gcggagggta tggccacatg gtaagcagcg agcatgtccc gtgtctcgac tatatccacc
     1921 aggacgaggc aacccgcccc ggccgcccta tagttcgccc agactgcggc caggttccgc
     1981 aggccgagcg cggtatggaa ggggtcctgc ttagggctgg ggtggcacca ccggagccag
     2041 tccatgtcga ttaccgcgtg ggccgtgccc atttccgcca gcagattgga tatctcaccg
     2101 gcgacggccg tcttacccac gcccacgggg ccggtcagga ctaatacggg aatggccgca
     2161 gcctcatcta ccataatgcc agtatatcaa ctaccaacca ggcccacacc ccggcctagc
     2221 gacgcgcggt ccactcctcg gacccgtatt tctcagccgc caaccggagc gcgacggctt
     2281 cctccgcacg ctccaacgtc gcacgttcaa ggctgatgga cagcgttcgc tcgaaggcgg
     2341 agactaacgc cgccagggct tcttcaggtg agatggcgcg gccaacttcg tgtcgtaagg
     2401 aggtcatgcg ctccggcagc ttggtgtagg cccgccgctt gcccgcctcc gatgcgtaag
     2461 caaataccga gacccaggcg tccaaatcga tgtccagcag tatcgagcca tgttgcagca
     2521 aggcaccccc acgcctggcc tgggcgctcc ctaccagctt cttaccgccc gccgtcagct
     2581 cgtaggccga agccgcgtcg aagcagaccg cgctgctccc ctccgctgcc tcccctgctt
     2641 cgtccagcac agctgcacgg cgtacggaca tggcgcgcag gagggccttg tccggcgctg
     2701 ccagcgacac cgtagccccc agcgtgcgca gcccctccac cagcgccgcg ctgatcttgc
     2761 ggtacgactc gaccaccccg cccgaaacgc gcgggtcgtt ttcgcgggcg atcaccgagt
     2821 atgtaagctc gtgctggtga agcacggccc gcccgccggt cagccgccgc accaccccga
     2881 acccgtgcgc ctcgcaggcc gcccggtcga tctcaccgag tgcctggttc gtgccgagcg
     2941 agagggtagc gggccgccat gtgtagaacc gcagcacagg cacaccaccc gctataaccg
     3001 agtcgagcag cgcctcgtca agagccatgt tgagcggccc cggcacgtcg aatccggcaa
     3061 gcagtcgcca cgattctacg cgcggtgggg cctgggtggg ggttggttgg agggatactg
     3121 tcatatatgc aaaagggcga gccgcctccg tgggccgctc cccctcatta tagcagtttg
     3181 cttcaggcgt gacgaggagg tttgtagcag gcacaaaccc tccgctaggg agtacgggtt
     3241 ggcatgcgag cgggctggcg gttcagccta tcgtcgccca ccatgaccag gatgtctacc
     3301 ggcatgccat cgaagttggt ggcgattggc gcgtcagccg gatcgccctg caagacggcc
     3361 gtatccgcca gtccaagctc ctgctttatc acctctatcg tgtgcggctt ctgcccgccc
     3421 gtgtagtcgg tgatggtggt gcgcgggtgc tggccctggt cggcagccga ggagaggtcg
     3481 gccacgttca agcccttttc ggcaagcagc tcgctcacga tgcgggccgt accgctggtc
     3541 gtcgtgccgt tctgcaccga cagccgggcg ttttcgttca ccagcctggg gtctgagaag
     3601 cactgcttta ccctgggccg gatgcgctcc cagatgggca tgagcacgtc cgcgccgctg
     3661 tcgagtatcg tttgctccac catagtggcg tcaatggcgc aggtggtaat ggagccggag
     3721 cctatgtcct gcgcgaggcc ggcaagcgcc ggaagctggc tcagcggcat gtccgtctcc
     3781 acggcgtcgg acagcttgtt cgccagctca cccagcttgg tgaggatgtt gaggttcatg
     3841 ccctgctgct tgagcgcgag caagacctgt tgctggcgct ggttgcggcc caggtcgcta
     3901 tccgcgtgcc gggagcgggc gtactggagg gccgtcttgc cgttcatgtg ttgcatgccc
     3961 gccggaatgt agatgcgggt cgcgccgtag ctgctggtgt aggtgccggg cgtgccggac
     4021 gtgccgtagc tggtgcttga gagcgggtac tcgttgtcca ccagcgggac gggcacgtca
     4081 atcttcagcc cgcccaacgc atcgaccacc tgctcgaagc cctggaagtt cacctgggcg
     4141 tagtagtcaa tcggtatccc gaaggtggcc gaaatggtgg acatcgccag cgttgggccg
     4201 ccgcccggca ggctttcggg gtcgagccgc caggcacggt cgcccagttg gtagctctgg
     4261 ttgatgcggc ccggcccgaa ccccggaatc gtcacgtaca ggtcgcgggg aatggagagc
     4321 aacgtcgccg tcttcgcggt aggatcgata tgcacgataa tctgcgtgtc ggcgcgcgtg
     4381 tcctcttccg taggccggta atcaaggccc agcaacagga tattgatagg ctcaccgctc
     4441 gcccaatcag gaggctcgac ctcaaccaac tgcggcgtgg catcgggcga cacggtgcca
     4501 tccgggtcca ccacgcgctt gttcacaggc gtcttcgata ttttgccggg tatatccagc
     4561 accggcttta ctgtgtcctg gtagaacacg tacccggtac cgccgcccac cagcatgagg
     4621 gccagcagga cgagtagcgt gatgcgtctg acgcgcttcc cccggttcgt gcctgcacgg
     4681 gctacggccg gccggcccgc cacgggctgc gttggcttgg tgacgcccgt ggtcacagac
     4741 ctggccctgt aggtaggttc gggccgccgg tactggtggt tggggttggg ccgtgggttg
     4801 gcgggggtag gtctgtcgtt agcagacgag ggtgtcttgc ggtggttggt ctggctcaat
     4861 cctgggtgct tcctcttgcg ccggtcgtat ggcaccggcc tcttatttac tgtgactctg
     4921 tagtgactct gtacgcttta tggtttcgcg gcggctgcgg gttgctcgct cgtgtcgtcg
     4981 aacttctcta tcagctcgtc ctgggagttg atgccgggcc gcttctcctc ttcggccagc
     5041 tcccgctgct cgcgggcctg ggtggtctcg gtctgctcgt cctgccggga gggcacgctc
     5101 gcctccgcct cacgcgaggt gtagccttcg ctgtaccgcc ggtacaggta gagataaacg
     5161 tcgcgcagta tggccgccac gggcaccgct attatcaaac cgaacagccc gcccacctgg
     5221 ctgcccacca ccaacgcgac catgatgatc gacgggtgca gcttcacgct atcaccctgt
     5281 atctttggaa cgagcaagtt gttctcaagt tgctggacaa gcacgtacac cgccagcacc
     5341 ttgagcacca tcacccaccc ctccgggtgg aaggccgcca ggatcaaacc cggtaccgcg
     5401 cccagcaccg ggccaactac cgggatgatc tccgtgatgc cggagatgat ggcgagcact
     5461 accgggtacc tggcaccgac aatcaacatg ccgatgaagg tcgccacgcc gacgatcagc
     5521 cccagcagca gttggccccg gatgtagctg ctgagcacgc cgttgatgat gcgcacgatg
     5581 cgccacacgt cggtgcgcca ggcgtggggc agcatattgt tgaaggcgtt gatgccccgg
     5641 tccttgtcct tcagcgtgta aaagagccag aacgggataa tcagcaggcc cacgatgaag
     5701 cccatcgcac ggctcacggc gctgagcgtg ttgagcacac cttgctgcac tgccgagccg
     5761 atcgcgccca ccgaattgcg caggccattc tctatcgact gccgcatgtc gtccgagatg
     5821 ggcagcgact ggtaccaggc catagcgtcc cggccccacc cctgcgcctg ctccgaatag
     5881 gtgggggcct gtttggccaa ctcgcccacc tggtgcccga ccaccggggc gagccaggct
     5941 atcaagcccc ataccgaccc gatgaatacc gcgtacacgg ccaggatagc gaccaccctg
     6001 ggcaggaacc ggctcagccg gtccacgagg gggagcatca ggtaggcgag cacgagggcg
     6061 atgacaaacg gccccagcgc gtcccacgac tggttgagca gccaccagac cacgaatatg
     6121 gcgataccca ccaggaccag ccgagcgcgt tggcggggcg tgactctgat tctcggtatg
     6181 agcggctcaa gcggttcgtc gcgcctggtt tgagcgtcgc cgttgctcaa tagtgcggtc
     6241 tccaaaggtc tgcttcaaga aggcttaggc aatcgaggtg ccactcgcgt attctaacac
     6301 acggcctggg ggctgaacag cgcaatccac agcatgccac actcgccccg gcggcccgcg
     6361 tatatagtaa cgacctggga gtcaaaactg ttcccgcacc ggggaacgcc ctgcaagggt
     6421 aacggcacgc tggtataatc gttctacaga agaacggatg cgagtttgag acgagtgagg
     6481 cagcaccggt atggacgaaa tggaaagcgg ctcttcgggg cgcgatgact ggcagtacgt
     6541 ggacggcaaa cccacacgcg cgggcgagca ggtccgcata ctccccggcg acccgcagca
     6601 ggcccgccag gtagggccag gctcccccgc aacccaacct cccggctctc gcagactgga
     6661 cccttacgag cctgccaaca tggacgcggg caggcgcggc gcggcgacga tagcgccaac
     6721 aggcagcgca gtaccctacc aggccgccca ggcgccgcca cccacgattg tgcaggtgaa
     6781 gcgcggcccc accgcctgcg tcatcctcgc gggtactttc gcggtactgg ctctttcgtg
     6841 cgcgctgctg gggttcgcca ccttccgggg tgggttggac ggctttggca agctcgacgg
     6901 catcctcccc agcttcagct tcggtatgac ccccaccgtc accatcgaca cgcagcagcc
     6961 cgccgtcatc agccagattc gcagcctgag caagctggaa acggtgaact accagcttga
     7021 gacagtggtc accgccaaga gcagcggccc cctgcccgac ctcctgacca gcgacaagat
     7081 actgctcgtg gctcacggcg aggtggtagc gggcatcgtc ttgagccgcc tgaaggaaga
     7141 agatgttacc gtcgtaagcg gcaccgtcac ggtcaatctg ccgcccgccg aggtgctgta
     7201 cacccgcctc gacaacgaca agacctacgt cgacgaccga cagaccggca tcttcagcag
     7261 ccccgacccc aacctggaaa ccgagttgcg ccgcgtagcc gagcgagaaa tcctgagcgc
     7321 ggccctggag gacggcatcc tgaccaaagc ctccgaaaac gcccagaccg tcctgcgcac
     7381 cctcatcacc
//
LOCUS       DASRWZ010000805         5241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_270964, whole genome shotgun sequence.
ACCESSION   DASRWZ010000805 DASRWZ010000000
VERSION     DASRWZ010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270964"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFH60_12790"
     CDS             <1..374
                     /locus_tag="VFH60_12790"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6314704.1"
                     /translation="SDTLLRNYAPVTAEAVYEVARAGDGAALGVTRAVGRYLALAIQQ
                     LVMAYDVECLVLGGGVSQQGEAFLQPILGELERLRQDSALAREMLQPDMIRLLPPDYG
                     AGVWGGVVLAGGDHPTHGRRH"
     gene            358..1974
                     /locus_tag="VFH60_12795"
     CDS             358..1974
                     /locus_tag="VFH60_12795"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HET6314705.1"
                     /translation="MAGGTEMIAHHASPVPLTVEQAVGQKTLLAFDGHEPPAEFLSLL
                     RRRHVGGVTLFRGPNVKDPEQVRKLTSALQLAASDAGQPPLLICADQEGGTLLALAGT
                     TPFPGNMALGAARSPELARRTGYAIGRELAAMGINVNYAPVCDVIVSSSAALVGPRSF
                     GEDPGEVGRLAGAVVAGLQQAGVAATAKHFPGHGDTSVDSHYGTPVLPHGEERLRMVE
                     LPPFVAAIEAGAKLVMTAHIALPALTGGLELPATMSSEVLRGLLRRELGFEGLIVTDA
                     MNMGAIQQGHGLVIDAIASLAAGADLLLLTDSGECQEGVYAGVLQAAQRGLLSQEETL
                     ASAGRVLDLKAWASGHEQPGLEVVRCEEHLALAYEVGAKSVTLVRDRGGILPLQLPAG
                     ARVVAIVPRPTDLTPADTSSFETPSFAAALRRYHPAVEEVVVPLDPSAEAVAALRDRL
                     AGCDLAVVGTINAGDHPGQAALVNALLEDGTRVLAVALRLPYDLAAYPAAPAYACTYS
                     LQPHSLEALADAFWGRIPFVGRLPVSVEGT"
     gene            2060..3100
                     /locus_tag="VFH60_12800"
     CDS             2060..3100
                     /locus_tag="VFH60_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET6314706.1"
                     /translation="MALIDEIMEQPDVLRHWLDTNLGKVEQIAEAVRQRDVQYVFLAA
                     RGTSDHAGVYAQYLLGSRNRLPVALAAPSLFTMYPGSPRLENALVIGISQSGQSPDIV
                     GVIEEGRRQGAITLAITNYDDSPMSEAAEMTLDIGAGPERAVAATKTYTAQLLALAAL
                     SVAMSGNRQEDLEALHRVPEAVAASLELERRAESIASKHASLDHCVVLGRGYNYATAQ
                     EWALKLKELAYVFTDVYSTADFQHGPIAIVEEGFPILAVAPEGAVFPDMLSLLHRLRD
                     EYKAELVVISDNEDALSLAQDGLRLPGGLPEWLTPIPAIIPAQLFCYHLTRAKGYDTE
                     QPRRLRKVTLTH"
     gene            complement(3317..>5241)
                     /locus_tag="VFH60_12805"
     CDS             complement(3317..>5241)
                     /locus_tag="VFH60_12805"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET6314707.1"
                     /translation="RRGLVMLAALAGAAASLLLMAALYVPDLDPSRIYYGTDTRASGL
                     LLGVSLAFLWAPWQPRSPGREWGWARKLLLDLGGLGALAGVIGFFLYLDEFQPFLYQG
                     GFASLGLLTVAAIGLTVHPRARLLPRLLGARLLRWVGLRSYGIYLWHWPIFMVTRPEL
                     DVPFDGPPLFILRVAATLAIAELSYRYVETPIRTAGLAGAWRALRARPELNWWDLAAR
                     RSATALGLLACIVICASVLSARPPEPPSYLSGDSVEIISSVDMSPGDFGPTFDLTAQP
                     SPETTPPSEATAMSTPTAEAPTAAQQDEVVPSPTETATTLPSATATATATRVPDALTN
                     TGVNLRTGPGKVYPILKVLGKGEPLAVLGKLRGNGSYVVRTSEGIEGWAYSVALTLNI
                     NPDDVPVVAAPPVGQPPPAAPTSAPANAAQGNSDPSPTSTHTLAATATPSPAGPPSEA
                     ANDKPTEVPADAPAGPPAPAPQPPPPGPPAITPVRITAIGDSVMQGAARELVRVLGAI
                     APTEVDAAKSRQVSQAINLIRGKRDAGTLGDVVVIHMGTNGTFTAKQFEQMMQLLSGV
                     RKVIFVNLKVPRQWEANNNKVITEGVQRYPNTVIVDWKAASAGQPTLFAKDGIHLQPA
                     GARLYTSLIAAQVQAP"
BASE COUNT          877 a         1648 c         1810 g          906 t
ORIGIN      
        1 ttagcgacac gctgctgcgg aattacgcgc ccgtgaccgc cgaagccgta tatgaggtcg
       61 ccagggcagg ggatggagcc gcgctggggg tcacgcgcgc ggtaggccgc tacctggccc
      121 tggcgataca gcaattggtg atggcttacg atgttgagtg cctcgtgctg ggaggcgggg
      181 tctcccagca gggagaggct ttcttacagc cgatactggg ggagctggag cgcctgcgac
      241 aggactctgc cctcgcccgt gagatgttgc aacctgatat gatccgcctc ctaccgcccg
      301 actacggtgc aggtgtgtgg ggaggcgtgg tgctcgcggg cggcgaccac cccacgcatg
      361 gcaggcggca ctgagatgat agcgcaccac gcctcacctg tgccgctcac ggtggagcaa
      421 gcggtgggcc agaagacgct cctggccttc gacgggcacg agccgcccgc cgagttcttg
      481 tcgttgttgc ggcgcaggca cgtgggtggg gtgacgctct ttcgcggccc caacgtgaag
      541 gacccggagc aagtacgcaa gctgacctcg gccctgcaac tcgccgcctc tgacgcgggt
      601 cagcccccac tgctgatatg cgccgaccag gaagggggta cgttgctggc cctggcaggc
      661 accacgccct tccccggcaa catggcgctg ggggctgctc gctcgccgga gcttgcacgc
      721 aggaccggct atgcgatagg acgggagctt gccgcgatgg gcatcaacgt caactacgcc
      781 ccggtgtgcg atgtgattgt cagttccagc gccgcgctgg taggcccgcg ctccttcggg
      841 gaggaccctg gggaggtagg caggttggcc ggggccgtgg tggccgggct gcaacaggcg
      901 ggcgtcgcgg ccacggccaa gcacttccca ggacatggtg acacgtccgt ggactcgcac
      961 tacggcacgc cggtgctccc acacggcgag gagcggcttc gtatggtcga actgccaccg
     1021 tttgtcgccg caatagaagc gggagcgaag ctggtgatga cggcccatat cgccctgccc
     1081 gccctcaccg ggggtctaga gctaccggcg accatgtctt cggaggtgtt gcggggcctg
     1141 ctacgccgcg agcttgggtt cgagggcttg atagtcaccg acgctatgaa catgggcgct
     1201 attcagcaag ggcatgggct ggttatcgat gctatcgcca gcctggctgc cggggccgac
     1261 ctgttgctgc tcacggacag cggcgagtgc caggaagggg tctacgcggg cgtgttgcaa
     1321 gcggcccagc gggggctgct ctctcaagag gagactttgg cgtcggccgg gcgtgtgctg
     1381 gacctgaagg cgtgggcaag cggacacgag cagccggggt tggaggtggt gcgctgcgag
     1441 gaacacctgg ccctggctta cgaggttggc gcgaagtctg tgacgctggt gcgcgaccgg
     1501 ggcggcatct tgcctctcca actaccggcc ggtgcgaggg ttgttgctat cgtgccgcgt
     1561 cccactgacc tcacccccgc cgatacctcc tcgttcgaga cgccgtcctt cgccgccgcc
     1621 ttgcgtcgct accaccctgc tgttgaagaa gtcgtggtgc ctctcgaccc atccgcggag
     1681 gccgtagcgg ctttgaggga ccggctggcg ggttgcgacc tggctgtcgt gggcaccatc
     1741 aacgcgggcg accacccagg acaggcggcc cttgttaacg cgctcctgga ggacggcact
     1801 cgcgtgctgg cggtggccct gcgcctcccc tacgacctgg cagcctatcc ggccgcgccc
     1861 gcctacgcct gcacgtacag cctacagccc cactccctcg aggcactggc cgacgcgttc
     1921 tgggggcgca tcccctttgt gggacgcttg ccggtaagcg tcgagggtac ttgatctacg
     1981 attggcctgc tttggcacaa cccttgactt gtagtaagtg cataatcgga caggccggaa
     2041 gctgaagtac gaggggaata tggcccttat agacgagatc atggagcagc cggatgtgct
     2101 gcgccactgg ctggatacca acctcggcaa ggtggagcag atcgccgagg cggtgcgaca
     2161 gcgggacgtg cagtacgtat tcctggcggc tcgcggcacg tccgaccacg cgggggtcta
     2221 tgcccagtat ctgctgggtt cgcgcaaccg cctgcccgtg gccctggccg ccccctctct
     2281 attcaccatg taccccggct cgccccggct ggagaacgcc ctggtaattg gcatttcgca
     2341 gtcgggccaa tcgcccgata tagtgggcgt catagaggag gggcggcggc agggggctat
     2401 tacgctcgcg ataaccaact acgacgactc gcccatgtcg gaggcggccg aaatgacgct
     2461 agacatcggg gccgggccgg aacgggcggt ggccgccacc aagacctata cggctcagtt
     2521 gctggccctc gcggcgctgt cggtcgccat gtccggcaac aggcaggaag acctcgaggc
     2581 cctccaccgc gtgcccgagg ccgtagcggc atcgctggag cttgagcgcc gggccgaaag
     2641 catagcctcc aagcacgcct cgctcgacca ctgtgtggtg ttggggcgcg ggtacaacta
     2701 cgccaccgcg caagagtggg ccttgaaact gaaggagctg gcttacgtct tcaccgacgt
     2761 ctactcgacc gccgatttcc agcacggccc catcgccatt gtcgaggagg gcttccccat
     2821 actggccgtg gcccctgaag gcgcggtctt ccccgacatg ctgtcgctgt tacaccggtt
     2881 gcgggacgaa tacaaggctg agttggtggt catctcggac aacgaagatg cgttgtccct
     2941 ggcgcaggat gggctgcgcc tgccgggcgg gctgccggaa tggctcaccc ccatcccagc
     3001 catcatcccc gcccagctat tctgctacca cctcacccga gccaaaggct acgacaccga
     3061 acaaccccgc aggctgcgca aggtcaccct aacccactag gtaggcacac ggcgagtaag
     3121 aggaagagac ggaaacgcaa agacgcaaag acgcaaagga cgcgcaaagg ttaagatggt
     3181 tgggtgagta caaaaaggag cagggtacct ggtgtcatac cctgctcctt tttgcttgga
     3241 acagctttat cctaatctct gcgcgtcctt tgcgtcctgt gcgtcttggc gttttcgtct
     3301 cttcccacgg caggtgctaa ggggcttgca cttgcgcggc gatgaggctg gtgtagaggc
     3361 gggcaccggc gggttggagg tgtatgccgt ccttggcgaa gagggtgggc tggcccgcgc
     3421 tggcggcctt ccagtctacg attacggtat tggggtagcg ctgcacgccc tcggtaatca
     3481 ccttgttgtt gttggcctcc cactggcgcg gcaccttcag gttcacgaat atcaccttgc
     3541 gcacgccgct gaggagctgc atcatctgct cgaactgctt ggcggtgaag gtgccgttcg
     3601 tgcccatgtg gatcaccacc acgtcgccca gcgtcccggc gtcgcgcttg cctcgtatca
     3661 gattgatcgc ctgggacacc tgcctgctct tcgccgcgtc cacctcggta ggcgctatgg
     3721 ctccgagcac ccgcaccaac tcgcgggcgg ccccctgcat gaccgagtcg cctatggctg
     3781 tgatcctgac gggcgttatc gcgggcggcc caggcggggg cggctggggc gcgggagcag
     3841 gtggaccggc aggtgcgtcc gcgggtacct cggtgggttt atcgttggcg gcctccgaag
     3901 gtggacccgc cggggacggc gtggccgtag cagcgagggt gtgcgtagag gtgggcgaag
     3961 ggtcgctgtt gccctgtgct gcatttgccg gcgcggaagt gggggccgcg ggcgggggct
     4021 gtccgacagg gggcgcggct accaccggca catcgtcggg gttgatgttt agcgtaaggg
     4081 ccaccgagta cgcccagccc tctatgccct cgctagtcct cacaacgtag gagccgttgc
     4141 cccgcagctt gcccagcact gccagcggct cgcccttacc caggaccttg aggatcgggt
     4201 aaactttgcc cggcccggtg cgcaggttca cgcctgtatt agtgagggca tcgggcaccc
     4261 gagtggctgt ggctgtggcc gtggcacttg ggagtgtagt agctgtctcg gtaggggagg
     4321 gcacaacctc atcctgttgt gcggccgttg gggcctcagc cgtaggtgtg gacatggcag
     4381 tggcttccga cgggggcgtg gtctcagggc ttggttgcgc ggtaagatcg aaggtcgggc
     4441 cgaagtcgcc gggcgacata tccacagaag agatgatttc gaccgagtcg cccgaaaggt
     4501 atgacggcgg ctccgggggc cgcgcgctta gtacggacgc gcagataacg atgcacgcca
     4561 gcaagccaag ggcagtggcg cttcgccggg ccgccaggtc ccaccagttg agttcgggtc
     4621 gtgcccgcag cgcgcgccag gcaccggcca ggcccgccgt gcgtatgggc gtctctacgt
     4681 agcggtaaga tagctccgcg atagccaggg tcgccgccac acgcaggatg aagaggggcg
     4741 gtccgtcgaa cggtacgtct agctccggcc gtgtgaccat gaatatgggc cagtgccaca
     4801 ggtagatacc gtacgagcgc agccctaccc accgcagcag ccttgcgccg agcaggcggg
     4861 gcaataggcg ggcgcgcggg tgcaccgtca gcccgatagc ggccaccgtg agcagtccca
     4921 gcgaggcgaa accgccctgg tacaggaagg gctggaactc gtcgaggtac aagaagaagc
     4981 cgatcacccc cgccagcgcg cccaggcctc ccagatcgag cagcagcttc cgcgcccagc
     5041 cccactcacg tcctggagag cggggttgcc agggtgccca taagaaggcg agcgacacgc
     5101 ccagcagcag ccccgacgct cgagtatcgg tgccgtagta gatgcgggag ggatcgaggt
     5161 cgggcacgta gagcgcggcc atgaggagga gagaagcggc cgcccctgcc agcgcggcca
     5221 gcatcaccag gccccggcgc a
//
LOCUS       DASRWZ010000806         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1231024, whole genome shotgun sequence.
ACCESSION   DASRWZ010000806 DASRWZ010000000
VERSION     DASRWZ010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231024"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VFH60_12810"
     CDS             <1..1179
                     /locus_tag="VFH60_12810"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET6314708.1"
                     /translation="SLREINKGLGGKNNGIPRQSGFDIAVASEAMAILALCKDIFDLR
                     GRLGRITVAQDAEGKPVTAEQLRAAGAMTVLLKDAIKPNLMQTLEGTPALVHCGPFAN
                     IAHGNSSILADYIGIKSTDYLITEAGFGADMGFEKFCDIKCRTSGLKPDAAVIVATVR
                     ALKSHSGRYKIVAGKPLDPGLTGENLPALEEGVENLVAQIENVRRMGLPAVVAINAFP
                     TDTEAEWSLIERVATQAGAFAAVPATHYANGGAGAADLAQAVVRATEQPAGFKFLYPL
                     EWGIKEKIHTIATQVYGADGVEYSEAAEARIDTITHMGHANLPICMAKTHLSLSHDPK
                     LKGRPRGFTLPIRDVRLSSGAGFVYPLCGEMMTMPGLPSEPAGAYMDIDEQGQVIGLH
                     "
     gene            1587..>2155
                     /locus_tag="VFH60_12815"
     CDS             1587..>2155
                     /locus_tag="VFH60_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET6314709.1"
                     /translation="MKPRHPNYTRLLKSALMCVCLAASSLSMLFADQVSGSVASQPAA
                     PTSPNGLVINEAFDSQTLTNEYFELLNTSGVTINLSTYVIYNKDGNTPLSNLDDQTIG
                     PGEYRVIGPVQLKTSSIGGASGLSQNDFLGLVNTSPSDTNIDVVNWGGAANPGWANYE
                     RFSNDFFKSTYAYMPPADDPRSLQRWPDGL"
BASE COUNT          437 a          742 c          612 g          364 t
ORIGIN      
        1 tccttgcgcg aaatcaacaa gggcctgggg ggcaagaaca acggcatacc ccggcaaagc
       61 ggcttcgaca tagccgtagc ttccgaagcc atggcgatcc tcgcgctttg caaggacatt
      121 ttcgacctgc gcgggcggct gggtaggatc accgtcgccc aggacgccga gggtaagccc
      181 gtcactgccg agcagctaag ggcggcgggc gcgatgaccg tgctcctcaa ggacgccatc
      241 aagcccaacc tgatgcagac gctggaaggc acccccgccc tggtccactg cggccccttt
      301 gccaacatcg cccacggcaa ctcctcaatc ctggcggact acatcggcat caagagcacc
      361 gactacctca tcacggaggc gggcttcggg gccgacatgg gcttcgagaa gttctgcgac
      421 atcaagtgcc gcaccagcgg actcaagccc gacgcggcgg ttatcgtggc taccgtgcgt
      481 gccctcaagt cccattccgg ccgctacaag atcgtggcag gcaagcccct cgaccccggt
      541 ctcacgggcg aaaacctccc cgcgctggag gagggcgtag agaacctcgt cgctcaaatc
      601 gagaacgtgc gccgcatggg cctcccggcg gtggtggcta tcaacgcctt ccccaccgac
      661 accgaggccg aatggtcgct catcgagcgc gtggcgaccc aggcgggcgc tttcgccgct
      721 gtacccgcca cccactacgc caacggtggg gccggggccg ccgacctcgc ccaggccgtc
      781 gttagggcca ccgagcaacc cgccggcttc aaattcctct acccgctgga gtggggcatc
      841 aaggagaaga tccacaccat cgccacccag gtgtatggcg cggacggcgt agagtactcg
      901 gaggcagcgg aggctcgcat cgacacgatt acgcacatgg ggcacgccaa cctgccaatc
      961 tgcatggcta agacccacct ctccctctcg cacgacccca agctgaaggg gcggccgcgc
     1021 ggctttacgc tacccatacg cgacgttcgc ctctcctccg gcgcgggctt cgtatatccc
     1081 ctctgcggcg agatgatgac catgcccggc ctcccctcgg agcctgccgg tgcctacatg
     1141 gatatcgacg agcaaggcca ggtaataggg ctgcactagc ggccatatcg ccacacggca
     1201 cgcacaggtg tggccccata gccctgtcgg agtgccctgc caccgtcgcc ctgtggctga
     1261 agccacgggc ttacaagagg caaaatctcc tgcggagact gaggaacatg agggtagtct
     1321 ccgaaggaga cttcgtccct ggtaagcccg ccaattcatt ggcagggcga aggccacagg
     1381 gcgacggtgg cagggcaccc ccacaggact ataccttttg gctagtgcgt ccttattgac
     1441 aggcacgcgc caaatcaagt atcatgtcct gtgcggctgc ggcgccgcct ttttagcacg
     1501 gggcgcgttc gtgcgttcct tgcagcccag cttttcccat tcccagcatt tgtatttcgt
     1561 cgggcgcaaa aggagactca accactttga agccgcgcca ccccaattac acgcgcctgc
     1621 tcaagtccgc gcttatgtgc gtgtgccttg cagcctctag cctgagcatg cttttcgcgg
     1681 accaggttag cggaagcgtg gcctcccagc ccgccgcccc aactagccct aacggccttg
     1741 tcatcaacga ggcattcgac agccagaccc tcacgaacga atacttcgag ctgctcaaca
     1801 ccagcggcgt cacgatcaat ctctccacct acgtcatcta caataaggac ggcaacacac
     1861 ccctctccaa ccttgacgat cagacgatcg gaccgggcga gtaccgcgtg ataggccccg
     1921 tccagctaaa aaccagcagc attggcggtg ccagcggact gtcacagaac gacttcctcg
     1981 gcctggtgaa cacgtcacct agcgacacga atatagacgt ggtgaactgg ggcggggcgg
     2041 ccaaccccgg ctgggccaac tacgaacgct tcagcaatga cttcttcaaa tcgacttatg
     2101 cctacatgcc tcccgccgat gaccccaggt cgttgcagcg ctggcccgat ggcct
//
LOCUS       DASRWZ010000807         4134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_614527, whole genome shotgun sequence.
ACCESSION   DASRWZ010000807 DASRWZ010000000
VERSION     DASRWZ010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614527"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="VFH60_12820"
     CDS             complement(<1..1269)
                     /locus_tag="VFH60_12820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F390 synthetase-related protein"
                     /protein_id="HET6314710.1"
                     /translation="MSRVAKALTLPLIVAHFAWARWRWRFLRGARLRRWQDRRARQVV
                     AYAQKHSPFYRWHWAGHNTLRWRTLPTVNKRLMMDNFSRFNTRGVGKDEAMSVALRAE
                     TTRDFSPTVRGLTVGLSSGTSGHRGIFLANGLEQAAWAGTVLARTLHRLRLRRLRVAF
                     FLRSNSNLYEKVGGLLVQFRFFDLMLPLAEAVDALNGYRPHIIVGPPSLLGLLAGEHL
                     ARRLRTGPQRLVSVAEVLEPQDMDYLESVFRAPVHQIYQCTEGLIAVSCERGSLHVQE
                     DIVALQMEPLPGDGERATPIVTDLWRRTQPIIRYRLNDVLKMQSARCPCGSPYRVIES
                     IEGRCDDICHFLAERGTRPFFPDTVRRMVLLSSPEITGYQAFQERPGHLRIHLELPPA
                     TSFDKVANNVRESVNTIVAQYGCMPAEVEIK"
     gene            complement(1266..2159)
                     /locus_tag="VFH60_12825"
     CDS             complement(1266..2159)
                     /locus_tag="VFH60_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6314711.1"
                     /translation="MMDSGVAGPALSTSLDAHSVRGSGPRLRYHVLDTGYCLASEHHI
                     MRGGARRKVKCHSIVGLLRHSQRGWFLWDAGYAPRMLAATRRLPYRLYRLATPLHIEA
                     GLAVAAQLPRFGLSTSDIGTVVLSHFHADHVAGLRDFPDARFIATEAAYDDVRSRRGV
                     SALRRAFIPSLLPPDFGRRATLISSFDGPDLPGLGPTHDLFGDGSALLVRLPGHARGQ
                     VGMLASTEGGPVLFAADGCWLTRQVRERRPPHPVTNLIVDDPHAVRSTIEGLHAFMQA
                     RPEVAVVPSHCPEAFAREVAR"
     gene            complement(2156..3145)
                     /locus_tag="VFH60_12830"
     CDS             complement(2156..3145)
                     /locus_tag="VFH60_12830"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6314712.1"
                     /translation="MEILVTGGTGFLGRHLAWGLLERGHGVRLMGRNFEGASDLTRSG
                     AVPEKADLRDRKAVIDACRGVGAVFHVGALSAPWGPARDFHDINVGGTASVLAGCREH
                     GVRRVVYVSSPSVVFDGRDHVNSREDAPYPRRFASVYSLTKKLGEDLVNATSRDGHLE
                     TVILRPKAMFGPGDQSLLPRLVEVARRKRLPQIGDGRNLVDLTYVENVVQALLLALDS
                     PAAPGKTYTITNGEHVPLWPTVRMVLRRTGLPDDLRPLPLTIALLVARLMEIGGTLRG
                     KEPLLTTYTVGILARTQTYDISAARRDLGYNPSVSVAEGIERTLGDFEQKKAA"
     gene            complement(3529..>4134)
                     /locus_tag="VFH60_12835"
     CDS             complement(3529..>4134)
                     /locus_tag="VFH60_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6314713.1"
                     /translation="KLTRVCVYDRANIGRSERGPSPRTSEQAVKELRTLLASAGVAGP
                     YLLVAHSHGGLHAQLFASKYRSEVVGMVMVDVTTYDIDQRYEAAITPAQVQQRRDLIE
                     QNREGVTYDAILASYAQVRDAGPLPEVPLLVLTHGRILEHPPGWPVEAVERAWREAQE
                     GLAKRSPKGRLIVAQNSGHFIHRDEPQLVIDSIREVLQAAR"
BASE COUNT          703 a         1357 c         1401 g          673 t
ORIGIN      
        1 cttgatttct acctcggcgg gcatgcagcc gtattgggct acgatggtgt tgacgctctc
       61 tcgcacgttg ttggctactt tgtcgaatga ggtggcgggg ggtagctcaa ggtggatgcg
      121 caggtggccg ggccgctcct ggaaggcctg gtagccggtg atttcagggc tggacaggag
      181 gaccatgcgg cgtaccgtgt cggggaagaa agggcgcgtg ccacgctcgg ccaggaagtg
      241 gcaaatgtcg tcgcaacggc cctcgatgga ctctatcacc ctgtaggggg agccgcaggg
      301 acaccgcgcg ctctgcatct tcagcacgtc gttgagccgg tagcggatga tgggctgcgt
      361 cctgcgccac aggtcggtga cgatgggggt ggcgcgctcg ccgtcgccgg gcagaggctc
      421 catttgcagg gccacgatgt cctcctgcac gtgcagcgac ccacgctcgc agcttaccgc
      481 gatgaggccc tcggtgcact ggtaaatctg gtgcaccggc gctcgaaaga ccgactcgag
      541 gtagtccatg tcctgcggct ccagcacctc ggccaccgac accagccgct gggggccggt
      601 ccgcagccgc cgcgccaggt gctcccccgc caggaggccg agcagagacg gcgggccgac
      661 gattatgtga gggcggtagc cgttgagcgc gtctacggcc tcagcgagcg gcagcatcag
      721 gtcgaagaag cggaactgca ccagcagccc gcccaccttt tcgtataagt tgctattcga
      781 gcgcaggaag aaggccaccc gaagccgcct cagcctcagc ctgtgcagcg tccgcgccag
      841 cacggtgccc gcccaggccg cctgctccag cccgttcgcc aggaatatgc ccctgtgccc
      901 cgacgtgccc gacgagaggc ccaccgtcag cccgcgcacc gtggggctga aatcgcgcgt
      961 ggtctccgcc cgcagggcca ccgacatcgc ctcgtccttg cccacaccgc gcgtgttgaa
     1021 gcggctaaag ttgtccatca tgagccgctt gttcacggtg ggcagggtgc gccagcgcag
     1081 ggtgttgtgc ccggcccagt gccagcggta gaagggcgag tgtttctgcg cgtaagccac
     1141 gacctgccgc gcccgccggt cctgccagcg acgcaacctg gccccgcgca ggaatcgcca
     1201 gcgccacctc gcccacgcga aatgcgccac tatcaacggc aacgtaaggg ccttcgccac
     1261 ccggctcacc gcgccacctc acgggcaaat gcctcagggc aatggctggg cacgacagcc
     1321 acctctgggc gagcctgcat gaaggcatgc aggccctcga tggtcgagcg caccgcgtgg
     1381 gggtcgtcca caatcaggtt ggtgacaggg tgtggcggcc tcctctcgcg tacctggcgg
     1441 gtgagccagc aaccatcggc ggcgaacagc accggccccc cttccgtgct cgccagcata
     1501 cccacctgcc ccctggcgtg ccccggcagc cgcaccagta gagcggaacc gtcgccaaac
     1561 aggtcgtggg tggggccaag tccgggcagg tcagggccgt cgaagctaga gatgagcgta
     1621 gccctccgcc cgaagtcggg cggtagcagc gaagggatga aggcccggcg cagggcgctc
     1681 acccctcgcc tggaccgcac gtcatcgtag gcggcttcgg tggcgatgaa gcgagcgtcg
     1741 gggaagtcgc gcagcccggc gacgtggtcg gcgtggaaat gggacaggac cactgtgccg
     1801 atgtccgagg tggagaggcc gaagcgtggc agttgggccg cgaccgccag gcccgcctcg
     1861 atgtgtagcg gggtggcgag cctgtagagg cggtaaggca ggcggcgggt ggccgcgagc
     1921 atgcgcgggg cgtaaccggc atcccaaagg aaccagccac gctgcgagtg ccgtagcagg
     1981 cccacaatcg agtggcactt caccttgcgc cgcgccccgc cccgcatgat gtggtgctcc
     2041 gaggcgaggc agtagcctgt atccagcacg tggtagcgca gccgtggccc gctgccacgc
     2101 acggagtgag cgtccagcga agttgagagg gcagggccag ctacgccgct atccatcatg
     2161 ccgccttctt ctgctcgaag tcaccgagcg tgcgctctat gccctcggcc accgaaacag
     2221 aggggttgta gcccaggtcg cgccgggccg cgctgatgtc gtaagtctgc gtgcgggcca
     2281 gtatgccgac cgtgtaggtg gtgagcagag gctccttgcc ccgcaacgtg ccgccaatct
     2341 ccatgaggcg ggcgacgagc agggcgatgg tcagggggag gggccgcagg tcgtcaggga
     2401 ggccggtccg ccggagcacc atgcgcaccg tgggccagag gggcacgtgc tcgccgttgg
     2461 tgatggtgta ggtcttgccg ggcgcggcgg ggctgtcgag ggccaggagg agggcttgca
     2521 ccacgttttc gacgtaggtg aggtccacga ggttgcggcc gtcgccaatc tggggcaggc
     2581 gcttgcggcg cgctacctcc acgagccggg gaaggagcga ctggtcgcca ggcccgaaca
     2641 tcgccttggg ccgcaggatg acagtttcga gatgcccgtc gcgactcgtg gcgttcacca
     2701 gatcttcgcc cagtttcttg gtgagggagt agaccgaagc gaaacggcgg gggtaaggcg
     2761 cgtcctcccg cgagttgacg tggtcgcgcc cgtcgaacac cacgctgggc gacgacacgt
     2821 agaccacgcg ccggacccca tgttcccggc agcccgccag caccgaggcc gtgccgccaa
     2881 cgttgatgtc gtggaagtcg cgggccggac cccacggagc agagagcgcc ccgacgtgga
     2941 acacggcccc caccccccgg cacgcgtcga ttacggcctt acgatcacgc aggtcggcct
     3001 tttcaggcac ggcccccgaa cgggtgaggt cggatgcgcc ctcgaagttg cgccccatga
     3061 ggcgcacgcc atgtccccgc tcgagcaggc cccacgccag gtgccgcccc aggaaccccg
     3121 taccgcccgt gaccagtatc tccaaaccgc gctccgctgg taccacttac gcacaaaaac
     3181 accccggcag agcataagaa acgccccacc agggctatta tagcaccggc actatcgcca
     3241 ggcaatgaag aggagacgaa aacgcaaaga cgcaaaggac gcaaagaacg cgcaaagagt
     3301 aggattgttt cgggtgtggg taggagcacg atgcatcgtc gctgcacggg caggaccaat
     3361 cccccatcct gtcatttctc accgcgttcg aaatgacagg atgggggatt ggtcctctgg
     3421 tcgcgatata caggccaggt accataggtt gtgctctggt agccctatac acccaaaccc
     3481 tacaccctct ttgcgcgttc tttgcgttct cctcatcact agtggggcct agcgcgcggc
     3541 ctggaggact tctcggatgg agtctattac cagttggggt tcgtcccggt ggatgaagtg
     3601 gccgctgttc tgggccacta tcagcctgcc cttgggggag cgtttcgcca ggccctcctg
     3661 ggcttcgcgc caggcccgtt cgacggcttc taccggccag cctggtgggt gctccaatat
     3721 gcggccgtgc gtcaggacga gcagcggcac ctccggtagc ggcccggcgt cgcgcacctg
     3781 ggcgtagctg gcgagtatag cgtcataggt aacgccctcc cggttctgtt cgatgaggtc
     3841 ccggcgttgt tggacctgcg ccggggtgat ggccgcttcg taacgctggt cgatgtcgta
     3901 ggtggtcaca tccaccatga ccatgcccac cacctcggac ctgtatttgc tggcgaacag
     3961 ttgcgcgtgg aggccgccgt gcgagtgtgc gaccagcagg tatggccccg ccaccccggc
     4021 gcttgccagc agcgtccgta gctctttcac cgcctgctcg ctggtgcgcg gtgatggtcc
     4081 gcgctcgctc ctgcctatgt tcgcccggtc gtacacgcat acccgcgtca gctt
//
LOCUS       DASRWZ010000808         1773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1367388, whole genome shotgun sequence.
ACCESSION   DASRWZ010000808 DASRWZ010000000
VERSION     DASRWZ010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367388"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1061)
                     /locus_tag="VFH60_12840"
     CDS             complement(198..1061)
                     /locus_tag="VFH60_12840"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET6314714.1"
                     /translation="MPDVSGCFQLVSTLGSTGGRDVADLDARQGSLGSEETQREDALI
                     ESAMPETGSETEIRERRVQWRTLLRAHYAMVLAAVLLATFGVLTFLALGLEPLAFDVP
                     VTLGLQRMQWGWFEGLMVAVSVFGFWPWMIVVPIAAMAILVALRVPKEAVWLGVAAIS
                     AVASEAVKVVVQRPRPTSDLVSVAAELHTYSFPSGHVTSYVTFYGFIFYLAYTGLRRG
                     WARTVILVLSGMMVLLVGPSRVWLGQHWASDALGGYALGFFMLLVLIQLHRAWATRAV
                     RAEQAQAAEAG"
     gene            1267..>1773
                     /locus_tag="VFH60_12845"
     CDS             1267..>1773
                     /locus_tag="VFH60_12845"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET6314715.1"
                     /translation="MYNQQSASPGSSASSLQDISNSWINALTKPYVPVYQQEAARATQ
                     NRILINVGIATAVAVLLGILGGLGGNMLGNVLGSLILTPLMFAIQVAVLYVASRALGG
                     TGTLEQQAWVQSTYWVPILMLRAIPIVGGFLYMIGWLYSLYLTYITVQTVHRLQSTQA
                     ILAMVLAFV"
BASE COUNT          343 a          634 c          499 g          297 t
ORIGIN      
        1 gtcggcgtgt ccggagaacc tggcgcgaga gtggagattg gcggggctgg cggcaggccg
       61 ggaacggctg gtcgacactc agtcggtcct cctgggggct tatgctgggc ccggtctttc
      121 tgaacggccg gcgccgtcac agcgcccatc gggagccatt atagcagcac cggctctacc
      181 accgcaaatg ccctgctcta cccggcctct gctgcctgcg cctgctccgc ccgcaccgct
      241 cgtgtcgccc aggcccggtg tagctggatc agcaccagca gcatgaaaaa tcccagggcg
      301 tagcccccta gcgcgtcgct tgcccagtgc tgccccagcc agacccgtga cgggcccacc
      361 agcagcacca tcatcccact cagcaccagt atgaccgtgc gcgcccagcc ccgccgcaga
      421 cccgtgtacg cgagatagaa gataaaaccg tagaaggtca cgtacgaggt cacgtgcccg
      481 ctggggaagc tgtaggtatg tagctccgcg gcaacggaaa ccaggtctga cgtcgggcgc
      541 ggccgctgca ccaccacctt caccgcctcc gacgccaccg cgctgatcgc cgcgacgccg
      601 agccacaccg cctccttcgg cactcgcagc gccaccagga tggccatcgc ggcgatcggc
      661 acgacgatca tccagggcca gaagccgaag acgctcaccg ccaccatcag cccctcgaac
      721 cagccccatt gcatgcgctg caatcccagc gtcaccggca cgtcgaaagc cagtggctcc
      781 aatcccagcg ccaggaaagt cagcacccca aaggtggcca gcagcaccgc cgccagcacc
      841 atcgcgtagt gcgcccgcag gagggttcgc cactgcacgc gcctctcgcg gatctcggtt
      901 tcgcttccgg tctcgggcat cgcgctctcg atgagcgcat cttcccgctg cgtctcttcg
      961 ctccccaggc tcccctgcct ggcgtccagg tcggccacgt ctctaccccc ggtcgatcct
     1021 aacgtgctga caagttgaaa acaacctgaa acatccggca agcaaaatgc gtacctatga
     1081 gtagttgaca gcgcccagcg cttgtgctag cataaggccg cttctccacc gggatagaag
     1141 caggcgagcc ggccccgaac cgctgcgggt caaattgtga cgcaactcgc aacaggacct
     1201 actaaatagg caatacgcaa tacgcaatag ccattacgca tcatcgaaag gagcatcggc
     1261 agtaacatgt acaatcagca gagcgcaagc cctggctcgt ctgccagtag cttgcaggac
     1321 atctccaaca gctggataaa cgccctcacg aagccctacg tgcccgtcta tcagcaagag
     1381 gcggcgcggg ccacccagaa ccgcatcctg atcaatgtcg gcatcgccac ggccgtggcc
     1441 gtgctgctgg ggatcctcgg cggtctcggc ggcaatatgt tgggcaacgt attgggcagc
     1501 ctgatcttga cgcccctgat gtttgccatc caggtcgcgg tgttgtacgt cgcctcgcgc
     1561 gctctcggcg gcaccggcac gctcgagcaa caggcatggg tgcagtccac ctactgggtc
     1621 cccatcctaa tgctccgcgc catcccgatc gtcggcggct tcctctacat gatcggctgg
     1681 ctctatagcc tctacctgac ctatatcacg gtgcagacgg tccaccggct ccagagcact
     1741 caggcgatcc tcgccatggt gcttgcgttc gtc
//
LOCUS       DASRWZ010000809         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1024941, whole genome shotgun sequence.
ACCESSION   DASRWZ010000809 DASRWZ010000000
VERSION     DASRWZ010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024941"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFH60_12850"
     CDS             <1..717
                     /locus_tag="VFH60_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314716.1"
                     /translation="VPAPTDTLVPVPTDTLVPVPTDTVAPAPTDTPVPAPTDTPVPPP
                     TNTRAPAPTDTARPQPTNTRVPAPTNTAVPPRPTATRAPAPPTSPPTRGIGDLVTGFN
                     GWTVLPLWVQTRDQLPSPDPNVVYRPRGVYWVLRVDARNTLDVPRSFGGTMDFVLRDA
                     NGRLYAELSDHGREPGVRDVARREGLNSLDMVLNQGQAGGTLLIYDIPRGVQPTQLVG
                     RIIEGNGVSPNGQVVWNLQR"
     gene            complement(767..1279)
                     /locus_tag="VFH60_12855"
     CDS             complement(767..1279)
                     /locus_tag="VFH60_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314717.1"
                     /translation="MLGHLLRQLTGGVVRLFLAAFVTYTALMYTPAGSRDVVIHPPRF
                     ASEGYVALTIERFELDKPWPLNFLAYLYEPGETTEVVFGKTYPKGMQVQLFGVGISGS
                     GILTGDFGRSLVIAKHVPVTDMYGSGFDLLFAVLLGMVLTFGYVATVQRLRLRDSRAL
                     TLDFADVMRA"
     gene            complement(1306..1611)
                     /locus_tag="VFH60_12860"
     CDS             complement(1306..1611)
                     /locus_tag="VFH60_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314718.1"
                     /translation="MNDRNEVEAKGVDVDLAGLQIRGSGILTGDFGYSVAMAKGTLAL
                     DVYGRGFDLAFAVLLGMVLTFGYVATVQRLGVRGGYAKPLDFADVTTAQGTYRREFV"
     gene            complement(2255..>2928)
                     /locus_tag="VFH60_12865"
     CDS             complement(2255..>2928)
                     /locus_tag="VFH60_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET6314719.1"
                     /translation="VSDRDSFEAARRVTREEGILIGGSGGTAMHAALQTARTMEEDEV
                     IVVLLPDTGRGYLSKLYNDAWMRENGFLDRLPTKARVRDLVVRSDRELPKLVTVPCNK
                     TVREAIDLLQQYSISQLPVTRPQSGDGNGRGPFTDVHEMIGSIQERNLLERVFRDPTL
                     IDAKVESVMDSPFPLVDVAEEVERVVPALLANNSAVLVEEAGQPVGLITRSDLLEFVA
                     HGQLG"
BASE COUNT          594 a          975 c          844 g          515 t
ORIGIN      
        1 gtccccgcgc ctaccgatac gcttgtcccg gtacccactg acacgctagt gccagtgccg
       61 actgatacgg tcgcgcctgc ccccaccgac acgcccgtac ccgcgccgac cgacacgccc
      121 gtaccaccgc ccactaacac gagggcaccc gccccgaccg ataccgccag gccccagcct
      181 accaacacga gggtacccgc ccccaccaat acggccgtgc ccccgcgacc taccgccacc
      241 agggcaccgg ccccgcccac ctcgccaccg acccgtggca taggcgacct ggttacgggc
      301 ttcaatggct ggacggtgct gccgctgtgg gtgcagacgc gcgaccagct acccagcccc
      361 gaccctaacg tcgtctaccg gcccaggggt gtctactggg tgctgcgcgt cgatgcccgc
      421 aacacgctcg acgtgccccg ctcgttcggc ggcactatgg acttcgtgct gagggacgcc
      481 aacggcaggc tctatgccga gctgtcagac cacggcaggg agccgggcgt gcgcgatgtg
      541 gcgaggagag aggggctgaa ctcgctcgat atggtgctca accagggcca ggcaggcggt
      601 actctgctga tatacgacat ccccaggggc gtgcaaccca cgcaattggt gggccgcatt
      661 atagaaggca acggagtgtc gcccaacggc caggtagtgt ggaacctgca aaggtagcgg
      721 cctgacgcag ctttgctcat caaacaagtt cccggcctta ctcgccttac gccctcatga
      781 catcagcaaa atccagggtc aatgcgcggc tatcacgaag tcgtagccgc tgcactgtgg
      841 cgacgtaccc gaaggttaga accatcccca gcagcaccgc gaagagcaag tcaaagccac
      901 taccgtacat gtccgttacc ggtacgtgct tggctattac aagtgacctc ccgaagtctc
      961 cggtcaatat tcccgatccg ctaatcccca cgccgaacaa ctggacttgc atacccttcg
     1021 ggtaggtctt gccgaatacg acctccgtag tctcaccggg ttcgtacaaa taagccagga
     1081 agttaagcgg ccagggctta tccaactcga acctctctat tgtcagcgcg acgtagcctt
     1141 cggaagcaaa acgcggcggg tgaattacta cgtcccgcga cccggcgggg gtgtacatga
     1201 gtgccgtgta ggtgacaaat gccgcaagga agagcctgac cactccgcct gtaagctgcc
     1261 gcagaagatg acccagcata tctcaccctc cgtcagggtt gctcatcaga cgaactctcg
     1321 ccggtaagtg ccttgcgctg tcgtgacatc agcaaagtcc agcggctttg cgtagccgcc
     1381 acgcacgccc agccgctgca ctgtggcgac gtacccgaag gtgaggacca tccccagcag
     1441 caccgcgaaa gctaagtcga agccacggcc atacacgtcc aacgccaggg tacccttggc
     1501 catcgcgaca gaatagccga agtcccccgt tagaatgccc gagcctctta tttgcaggcc
     1561 cgcgaggtcc acatccacac ccttcgcttc tacctcgttc ctgtcgttca cctgccttgt
     1621 gtccagaggg tcaagcagcc atgtcatgta attgtaaggc cagggcttat agaggtgata
     1681 tgcaccctcg aacgggtggc ggaggctggt accgtcgatc ggccgtgtgc cgacgcgctt
     1741 gtcgggtttc tgtgacaggg cttcccgaag gcggacaaca cctgtaatct cgtctatagc
     1801 gccgccgggc gaatagacta ataggtggta cagcacacac gtggcagcca gcagggagac
     1861 aataccccca attgcgagcc gtacaaaatg acgtgccata agtcctgcct gtgcctacac
     1921 tccagcttcc gccgtgcctt acgctgttag tacaacccag gcgcgggggg tggttcactc
     1981 ggggcgcgcg ggaccgcgtg ctgctatagg ggcggcacgc accggggtgg cagaccgaga
     2041 aagtagtgat gccaatctct agcggagggc gcgtccccta gagattggca tcttctgact
     2101 ataaaacgcc ggggtcgcgc aaaagatagg ggtaaaacaa cgaaatccgc aaaaattggt
     2161 tcgtaccaac gacccatccg cccgctaaaa gcaacccctc gattggctga acacccacaa
     2221 atccgaaatt ctaaattgca aattccaaat ctcttcaccc caactggccg tgcgccacga
     2281 actccagcag gtcgcttctc gtaatcaggc ccacgggctg gcctgcttcc tccaccagca
     2341 cggcggagtt gttggccagc agggcgggca ccacccgctc cacctcctcg gctacgtcca
     2401 cgagcgggaa ggggctgtcc atcaccgact cgaccttggc gtctatgagg gtggggtctc
     2461 ggaacacgcg ctctagcagg ttgcgctcct ggatggagcc tatcatctcg tgcacgtcgg
     2521 tgaaggggcc gcgcccattg ccatcgcccg actgcggccg cgttacgggt agctgcgaga
     2581 tgctgtactg ctgcaagagg tcaatcgcct cacgcaccgt cttgttgcag ggcaccgtca
     2641 ccagcttggg tagctcgcgg tcactacgga ccacgaggtc gcgcacgcgg gccttggttg
     2701 gtaggcggtc gaggaagccg ttttcgcgca tccaggcatc gttgtagagc ttcgataggt
     2761 agccgcgccc ggtgtcgggg agcagcacca cgatcacctc gtcctcctcc atcgtgcgcg
     2821 ctgtctggag ggcggcgtgc atagcggtgc cgccggagcc gccgatcagg atgccctcct
     2881 cgcgggtcac gcgccgggcc gcctcgaacg agtcgcggtc gctcaccg
//
LOCUS       DASRWZ010000810         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_271400, whole genome shotgun sequence.
ACCESSION   DASRWZ010000810 DASRWZ010000000
VERSION     DASRWZ010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271400"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            488..1888
                     /gene="murF"
                     /locus_tag="VFH60_12870"
     CDS             488..1888
                     /gene="murF"
                     /locus_tag="VFH60_12870"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET6314720.1"
                     /translation="MYTLNDVLEGTKGRLDEATGALAMTGLGFNSVAIDSRAALSGAL
                     FVALMGERVDGHEFVLDAAGHGARGALVREDWTAPATLPPGMALIRVPDPLAALQSFA
                     AWWRARFDVQVVGVTGSVGKTSTKEVIAGVLSTRYNTLKSEGNLNNEIGCPLTVLRLG
                     TEHQKAVLEMGAGYQLGELTLLCEIARPEIAVVTVVGPVHLERMGTIENIALNKSELV
                     RALPPSGTAVLNGDDPLVRGMQEVASAGVLLYGLDPQWDLYATDIEGQGLQGVHFTLH
                     VKATGERWRIRLPLLGRHSVHTALAAAGAGMAAGMDWDSIIMALQRLDAQVRLLVVPG
                     YNGSTLIDDSYNASPDSTMAALNLLEEMPGQRVAVLGDMLELGSYEREGHLKVARRVA
                     GVAHRLVVVGSLGRLIGEEALRSGMPQDRVFFAADNEQVAVYLKRVLKAGDYVLVKGS
                     RGLKMEQIVDALKVDG"
     gene            1915..3045
                     /gene="mraY"
                     /locus_tag="VFH60_12875"
     CDS             1915..3045
                     /gene="mraY"
                     /locus_tag="VFH60_12875"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET6314721.1"
                     /translation="MSLVAGVANQAAGALPDVQSLFGDVVAQTAVLTSTVTPTPTPTA
                     ESPFTLPLPRDVMVRGLTLGAVSFLIGLMIGRPVINWLRDRRIGKSIRIEGPASHQIK
                     TGTPTMGGIIFLIPLVIVIAIFMDIPRFLSLLLPLAIVVSCGVLGAFDDLLSTVVRDR
                     GGLAGRFKMAWLLVIAILASWVIYFLLGRHNVGVPFMGLQPVDATVYMVLAVLAIVGT
                     ANTVNLSDGLDTLAGGTTAIAFVSYGIIAYLQGQEPVVLICFATVGALLAFLWFNAHP
                     AQVFMGDTGSLTLGALLAVCALMTDQWLLLPLVGIVFVMEGISDILQVLYFKLTKGKR
                     LFRMAPIHHHFEQLGWSETQVTMRFWLISMLAGMLGVALAMV"
BASE COUNT          569 a          996 c          965 g          617 t
ORIGIN      
        1 gtagcgaagt cgcctttgga gactagagct actgcgcaaa ctgtaggaag caagcacaca
       61 gggtagtcct tatcattctg agcgtagcga agaatctcgt ccttcaccga ggtgtggccc
      121 ttttccgtag cacatggcag cgccttgtac tgtgcaggca cgaagaaaag gggtatttgg
      181 gtgctgaagg acgagattct tcgctacgct cagaatgaca aagtgaaact cggtcccgca
      241 ctggcggcat ttagcagaga agccccagtc tccgaaggag acttcgctac ttcccagccc
      301 gtggcttcag ccacaggcgt ccatgcaggg caacatggca gagcatacaa tccaggaggt
      361 cgcaatttga acccacccta ccgcataccg cccccattgc ccgtttcctc aacatcctgt
      421 atccgtctac ttggcccatg acgaggccaa atcaggccgt ggccacccag taacacccgt
      481 aactcccatg tacaccctca acgacgtatt agaaggcacc aagggccgcc tggacgaggc
      541 aaccggcgca ctcgccatga ccgggctggg cttcaactcg gtggcaatcg actcgcgggc
      601 cgcgctgtcg ggtgccctgt tcgtggccct catgggcgag cgggtagacg gccacgagtt
      661 cgtgctggac gccgccgggc atggcgctcg cggtgccctg gtgcgcgagg actggaccgc
      721 tcccgctacg ctgccgcccg gcatggccct catacgcgtg cccgaccccc tcgccgcgct
      781 gcaaagtttc gccgcctggt ggcgcgcccg cttcgacgtg caggtggtcg gcgttacggg
      841 gagcgtgggc aagacctcca ccaaggaagt gatagccggg gtgctctcca cgcgctacaa
      901 caccctcaag tcggagggca acctgaacaa cgagataggc tgccccctca ccgtgctgcg
      961 gctcggtacc gagcaccaaa aagccgtgct ggagatgggc gcgggctacc aactgggcga
     1021 gcttaccttg ctgtgcgaga tagcgcgtcc cgaaatcgcc gtggtcacgg tggttggccc
     1081 ggtccacctg gagcgcatgg gcacgataga gaacatcgcc ctcaacaaga gcgagcttgt
     1141 gcgcgccctt ccgccctccg gcacggccgt gctcaacggc gacgaccctc tggtgagggg
     1201 catgcaagag gtagcgtctg ccggggtact cctctacggc ctcgacccgc agtgggacct
     1261 ctacgctacc gacatagaag ggcagggctt gcagggcgtg cacttcaccc tgcacgtcaa
     1321 ggccaccggc gaacgctggc gcatccggct gccgctcctg gggcggcaca gcgtgcacac
     1381 tgccctggcg gctgccgggg cgggcatggc cgcgggcatg gactgggact cgataatcat
     1441 ggctttgcag cggctcgacg cccaggtgcg cctgctggtg gtgcccggct acaacggctc
     1501 taccctcata gacgacagct acaacgcctc gcccgactcc acgatggccg cgctcaacct
     1561 gctggaagag atgcctgggc agcgggtcgc cgtgctgggt gatatgctgg agctgggcag
     1621 ctacgagcgc gaggggcacc tcaaggtggc gcgcagggtc gccggggtgg cccaccgcct
     1681 cgtcgtggtg ggcagcctgg ggcggctcat cggagaggag gcgctgcgct ccggcatgcc
     1741 ccaggaccgg gtgttcttcg ccgccgacaa cgagcaggtg gccgtctacc tcaagcgcgt
     1801 gctcaaggcg ggcgactacg tgctggttaa aggctcgcga ggcttgaaga tggaacagat
     1861 tgtggatgcc ttgaaagttg atggataacc ttgcctgatt ttcttctccc atgcgtgtcc
     1921 ctggtagccg gcgtcgccaa tcaggcggcc ggtgcgctgc ctgatgtgca atcgctgttt
     1981 ggcgatgtgg tagcccaaac agccgtattg acgtccacgg tgacacccac tcccacgcct
     2041 accgccgagt cgccgttcac cctgcccctc ccaagggacg tgatggtgcg cggcctgacg
     2101 cttggtgccg tctctttcct catcggcctc atgataggta ggcccgtaat caactggctg
     2161 cgcgaccgcc gcatcggcaa gagcatacgc atcgaaggcc ccgccagcca ccagatcaag
     2221 acgggcaccc ccactatggg cggcatcatt ttcctcatac cgctcgtcat cgtgatcgcc
     2281 atattcatgg acataccgcg cttcctgtcg ttgctgttgc ccctcgccat cgtcgtgtcg
     2341 tgcggcgtgc tgggggcctt tgacgacctc ctgagcacgg tagtacgcga ccgagggggc
     2401 ctcgcggggc gcttcaagat ggcctggttg ctggtgatcg ccattttggc gtcctgggtg
     2461 atctatttcc tgctcggtag gcacaacgtg ggcgtgccct tcatggggtt gcagccggtg
     2521 gacgcgaccg tctatatggt gctggccgtg ctcgcaatcg tgggcaccgc caacacggtg
     2581 aacctcagcg acggcctaga cacgctggcg ggcggcacca cggccatcgc tttcgtgtcc
     2641 tacggcatca tagcctacct gcaaggccag gagccggtgg tcctcatctg tttcgccacc
     2701 gtgggcgcgt tgctcgcgtt cctgtggttc aacgcccacc ccgcccaggt gttcatgggc
     2761 gacaccggct cgctcacgtt gggcgcgctg ctcgccgtgt gcgccctcat gacggaccaa
     2821 tggttgctgt tgccccttgt tggtatagtg ttcgtaatgg agggtatttc cgacatcctg
     2881 caagtgttat acttcaagct gaccaagggc aagcgcctct tccgcatggc ccccatccac
     2941 caccacttcg agcagcttgg ttggtccgaa actcaagtca caatgcgctt ctggctaatc
     3001 agcatgctcg ccgggatgct gggagtagca ctggcgatgg tataatttta gattttggat
     3061 tttaggttgt cgaggcgtct agaatccaaa atctaaaatc taaaatctag aatcgtatgt
     3121 cttttcctta cgccgacaaa aacgtac
//
LOCUS       DASRWZ010000811         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1162719, whole genome shotgun sequence.
ACCESSION   DASRWZ010000811 DASRWZ010000000
VERSION     DASRWZ010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162719"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..3050
                     /locus_tag="VFH60_12880"
     CDS             375..3050
                     /locus_tag="VFH60_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314722.1"
                     /translation="MNRRVLASLLGLGALALAVLPASTGHVAATSNTNAATNASVASQ
                     RAGDSGARVVADSPIVDSPNAVDARQNASRSVPAAPSLSGPSAVGDLASSSFDRMPAW
                     SAPAGYEGSWSATSGRLQQTGDASGDGSLSHDTAALANNVATRDGTVRVVAYPTGGEP
                     FGVLFRGTASGHYRVTLYPNAPNNGPKAVVERVSTASKSGTRVSSVVQASASAWQGYT
                     PGRWHDVRLAMQGSRVELFVDGANVLSYTDTAPAAGWSGLWGTADQGVSFDNFALSSA
                     ADFTVGEAPSYEAPASNDAPAAPLAGWSSPKNVTNSPLYDQTPALAVSASTGAVTVLW
                     MRDDPQNVTTPASYLMASNGSASTDFSNLTVSTALPRALGNIRAARDSEGKVHAAFFE
                     LTLYSCGRYSQLDAKGNAVLTEDVPGTCGTNRKNVAIAVGPTGTVHILFGSNNRDAYY
                     YQRSADGKWTVQGEEVVTDAVADLAIVESTKGVVMAAYKAPSLGGYSDIVTARRDAAG
                     TWVKENVSSQCCNSCADNSRTYLPSLAAAPDGGLRMSWSDESCEARGELAYNDIYYRE
                     WVPGTGWEGKPMVRVVKNAGHSYYNDIAVDSSGKAHIIWADTTGREYSNFLLMYASGS
                     GTTFSAPVSPWDAWNGVSFSKEPSIETSTGYAHVVFGSNRDDFLKENYYTNLPITDGP
                     TPTPQPTATPVACLFTDVCTTDYFYDAVRALNKAGVLSGYAANPPCDKGTPCFRPYDN
                     MTRAQSVKVVALGAGWRLLEPRTNSFADVAVGSTFYQYVETAAARGVISGYQCGGANE
                     PCDSEKRPYLRPGGKVTRGQFTKMVVSGFDMQLHPGNTAHFADVPVGSTFFQYIETAY
                     TGGLISGYGDGNFRPNDNVTRGQAAKVVYIARGNK"
BASE COUNT          589 a         1064 c          935 g          525 t
ORIGIN      
        1 cgctacgctc agaatgacaa aggccgggct tggtcccgca cgggcggcac gttgcggata
       61 agtcctagtc tccgaaggag acttcgctac ttcccagccc gtggcttcag ccacagggcg
      121 tcgtacgggg cgtcaccgca attcactgaa atgacagtac ttaccgtacc gcatgattag
      181 gacccttgct gctttttgtc gcgtagccac ccacaacggg gcataagtag taggactttg
      241 ggttagtggt gtgtggctac aaatagctaa gctcagcggt accatcgggc tactgtgcgg
      301 aagcgtcggg ttggatagac tgaggatgtc atattcctta ccatcatcaa gcgcctattc
      361 ggaaaggaca tccaatgaac cgcagagtct tagcaagtct gctaggcctg ggggctttgg
      421 ctcttgccgt gctcccagcc agcacaggac atgtagccgc cacctccaac accaatgcag
      481 ccaccaacgc ctccgtcgcc tctcagcgcg cgggagattc gggtgcgcgg gtcgtagccg
      541 attcgcccat cgttgattcg cccaacgcgg tagacgctcg ccaaaacgcc tcccgttcgg
      601 tgcccgccgc gccttcgctg agcggccctt ccgctgtggg cgatctcgcg tcgagcagct
      661 tcgaccggat gccggcatgg agcgcccccg caggttatga gggtagctgg agcgccacgt
      721 cgggtcgctt gcagcagacc ggcgatgcct ccggcgacgg cagcctctcc catgacactg
      781 ccgccctggc caacaacgtg gcgacccgcg acggcaccgt gcgcgtggtg gcttacccca
      841 ccggcggcga gccgttcggc gtgctcttca ggggcaccgc ctccggccac taccgcgtga
      901 ctctctaccc taacgccccc aacaacggac ctaaagccgt ggtagagcgc gtgtccacag
      961 cctccaagag cggcacccgc gtctcgagtg tggtccaggc ttccgcgagc gcgtggcagg
     1021 gctacacccc cgggcgctgg cacgatgtgc ggttggcgat gcaaggctcc cgtgtggagc
     1081 tattcgtgga cggcgcaaac gtgctcagct acaccgacac cgcgcccgcc gccggttggt
     1141 ccggcctatg gggcaccgcg gaccagggtg tgtcgttcga caacttcgcc ctatcgtcgg
     1201 ccgccgactt cacagtaggt gaggccccct cctacgaagc ccccgctagc aacgatgcgc
     1261 ctgccgcgcc ccttgccggt tggtcgtcgc ctaagaacgt gaccaacagc cccctctacg
     1321 accagacgcc tgctctggcg gtatccgcct cgacgggcgc ggtgaccgtg ctctggatgc
     1381 gcgacgaccc tcagaacgtg accacccctg ccagctattt gatggcaagc aacggcagcg
     1441 cctccaccga cttcagcaac ctgacggtaa gcaccgccct cccaagggcg ctcggcaaca
     1501 ttcgtgcggc ccgcgactcc gagggcaagg tccacgccgc cttcttcgaa ttgacgctct
     1561 attcctgcgg ccgctactcg cagctcgacg ctaagggcaa cgcggtacta accgaggatg
     1621 tacccggcac ttgcggcacc aatcgcaaga acgtggctat cgcggtcggc cccaccggca
     1681 ccgtacacat cctgttcggc tcgaacaaca gggacgcgta ctactaccag cgctccgccg
     1741 acggcaagtg gaccgtgcag ggcgaggaag tcgtcacgga cgccgtggcc gacctcgcaa
     1801 tcgtggagtc gaccaagggt gtggtcatgg cggcttacaa ggcccccagt ctcggcggct
     1861 acagcgacat cgttaccgcc cgccgcgacg ccgccggcac gtgggtgaag gagaacgtct
     1921 catcgcagtg ctgcaattcc tgcgccgaca actcgcgcac ctacctgccc agcctggccg
     1981 cggcccccga cggtgggcta cgcatgtcct ggtctgacga aagctgcgag gctcgcgggg
     2041 agctggctta caacgacatc tattaccgtg agtgggtgcc gggcaccggg tgggaaggca
     2101 agccgatggt gcgcgtcgtc aagaacgccg ggcattccta ctacaacgac atagcagtgg
     2161 acagctccgg caaggcgcac atcatctggg ccgacaccac gggccgcgag tactccaact
     2221 tcttgttgat gtacgcctcc ggcagcggca ctacgtttag cgccccggtc agcccgtggg
     2281 atgcctggaa cggcgtcagc ttctccaagg agccttccat cgagacttcg accggctacg
     2341 cacacgtggt cttcggctcg aaccgggacg acttcctcaa ggagaactac tacaccaacc
     2401 tgccgatcac agatggtcct acccctacgc cgcagcccac cgccaccccg gtagcctgtc
     2461 tcttcaccga cgtctgcacg accgactact tctacgacgc ggtccgggcg ctgaacaagg
     2521 cgggcgtgct ctcaggctac gcggccaacc ccccgtgtga caagggcacc ccctgcttcc
     2581 ggccctacga caacatgacg cgggcgcaga gcgtgaaggt tgtggccttg ggcgcgggct
     2641 ggcggctgct cgaaccgcgc accaatagct tcgccgacgt ggcggtaggg tctaccttct
     2701 accagtacgt ggaaacggcg gcggcacgcg gcgtgatctc cggctaccaa tgcggcggcg
     2761 cgaacgagcc ttgcgatagc gagaagcgtc cctacctgcg gcccggcggc aaggtcactc
     2821 gcgggcagtt caccaagatg gtggtgtcgg gcttcgacat gcagctccac cccggcaata
     2881 cggcccactt cgcggacgta ccggtaggct ccaccttctt ccagtacatc gaaacggcct
     2941 acaccggcgg cctcatcagc ggctacggcg acggcaactt ccgccccaac gacaacgtca
     3001 cccgcggcca ggccgccaag gtggtctaca tcgcacgggg gaacaagtag ctagaataga
     3061 cgttaggaga cgaaaccgca aaggcgcaaa gagcgcaaag tttaatgatt gat
//
LOCUS       DASRWZ010000812         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_409811, whole genome shotgun sequence.
ACCESSION   DASRWZ010000812 DASRWZ010000000
VERSION     DASRWZ010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409811"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1365
                     /locus_tag="VFH60_12885"
     CDS             154..1365
                     /locus_tag="VFH60_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HET6314723.1"
                     /translation="MLSSARHSPSDTTRMAARVHTITESVFSEMSRLAVEHNAVNLSQ
                     GFPDFPAEEWLKEAAREAVARDVNQYAPSQGTLRLRKAIAAKAESFMGLTFDPAQEIT
                     VTNGATEAIYDVIQALVGPGDEVIVFEPYYDSYVPSIVFAGGTPRFVTLDSPDWSFDR
                     ALLASLFNERTRAIILNTPHNPTGKVFSQDEMELIADLCKRYDALAITDEVYEHLVFD
                     GHRHVYMAALPDMRDRTVTISSASKTFSVTGWKVGYVLAPPDLTEALRRLHQFVTFCC
                     SAPMQEAVALGIETAPERGYYEQLQQAYNARLDKLHGYLQQAGLNPIRPQGTFFIMSD
                     ISALGFENDVEFARYMTTEVGVACIPPSAFYSNPQSGTHLARWCFAKRDSTLDAAGDR
                     LMAWATRQDKA"
     gene            1400..2536
                     /locus_tag="VFH60_12890"
     CDS             1400..2536
                     /locus_tag="VFH60_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012576214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6314724.1"
                     /translation="MDFNLTAHQIELRNRIRNFAEKELAPTVSERDEAGTFDRWVFDK
                     CVEAGLAGLPFPEAYCGGGGGILDYIIALEEIGRVESAQVLVLASHVAPMTCLLQHGT
                     EEQKQRWLAPMCKGETLACFSLTEPGAGSDPASMKSRAVREGDGYVLNGRKTFATNSG
                     EADVYILFAMTDPEAGGRGISAFIIPRDTPGLTFGQPIQKMGVRTSVQREIYLEDVRL
                     PAEHLLGQEGQGLRIALAALDVGRIEIAAQGVGLSQGAYDFALNHAKGRVQFGQAIGQ
                     NQAISFMLADMHVATEAARMLTYRAAMSYDEGKPFAKEAAMAKLLATDTAMKVTTDAV
                     QILGGFGVTKASPAERFKRDANVTQIYEGTNQVQRMVIAGHIMK"
BASE COUNT          526 a          845 c          836 g          458 t
ORIGIN      
        1 tggaatttcg gatttcggat ttcgaatttg gcaggtatcc aatcaaattc gcaattcgaa
       61 attccaaatt ccaaattcgt ccatcgcaac tcccgtgccc gcgtacggag gctatgttat
      121 aatgtctcac cccacgagca ggaggctaac gccgtgcttt cctcagcccg ccattccccc
      181 agcgacacca cgcgcatggc agcccgcgtc cacacgatta ccgagtcggt cttctccgag
      241 atgagcaggc tcgccgtcga gcacaacgcc gtcaatctct cccagggctt ccccgacttc
      301 ccggcggaag aatggctcaa ggaagccgcg agagaggctg tggcccgcga cgtgaaccag
      361 tacgcaccca gccagggcac gctgcggctg cgcaaggcga ttgcggccaa ggcagagagc
      421 ttcatgggcc tcacgttcga cccggcgcag gaaatcaccg tcaccaacgg ggccaccgag
      481 gctatctacg acgtgataca ggcgctggtc gggcctggcg acgaggtgat cgtgttcgag
      541 ccttactacg actcgtacgt gcccagcatc gttttcgcgg gcggcactcc caggttcgta
      601 acgctcgatt cgcccgattg gagcttcgac cgcgccctgc ttgcctccct gttcaacgag
      661 cgcacccgcg cgatcatcct gaacacgccc cacaacccta cgggcaaggt cttctcgcag
      721 gacgaaatgg agctaatcgc ggacctgtgc aagcggtacg acgcgctcgc catcaccgac
      781 gaggtgtacg agcacctggt gttcgacggc caccggcacg tctacatggc cgccctgccc
      841 gatatgcgcg accgcaccgt gaccatcagc tccgcaagta agaccttctc ggtaacgggc
      901 tggaaggtcg gctacgtgct cgccccgccc gacctcaccg aagcgttgcg ccgccttcac
      961 cagtttgtga cattttgctg ttccgcaccc atgcaagaag cggtggccct cggtatcgag
     1021 accgcgccgg agcggggata ttacgagcag ctacagcagg cgtataacgc ccgcctggac
     1081 aagttgcatg gctacttgca acaggcgggc ctcaacccta tcaggccgca gggcaccttc
     1141 ttcatcatgt ccgatatctc cgcgctcggc ttcgagaacg acgtggagtt cgcccgctat
     1201 atgactaccg aagtgggtgt ggcctgcatc ccaccttcgg ctttctacag caacccgcag
     1261 tctgggacgc acctggcccg ctggtgcttc gccaagcgcg acagcaccct ggatgcggcg
     1321 ggcgacaggc tcatggcgtg ggccacgcgc caggacaagg cttgagcacc ctggcactta
     1381 tccgatatag aaggaaagca tggacttcaa tctcacagcc catcaaatcg agctaaggaa
     1441 caggatacgg aacttcgcgg agaaggagct tgcccccacc gtatcggagc gggatgaggc
     1501 cgggacgttc gacaggtggg tgttcgacaa gtgcgtggaa gcggggctgg cagggctgcc
     1561 cttccccgaa gcgtattgcg gtggcggggg cggcatcctg gactacatca tcgcgctgga
     1621 ggagataggg cgggtcgagt cggcgcaggt gctggtgctg gcctctcacg tcgcgcctat
     1681 gacctgtctg ttgcaacacg gcacagagga gcagaagcag cgctggctcg ccccgatgtg
     1741 caagggggag accctggctt gcttcagcct caccgagccg ggcgcggggt cggacccggc
     1801 ctccatgaag tcgcgggcgg tgcgcgaggg cgacggctac gtactgaacg ggcgtaagac
     1861 gttcgccacc aactcgggcg aggccgacgt gtacatcctc ttcgcgatga ccgaccccga
     1921 ggcgggaggg cggggcatca gcgctttcat tatcccccgc gacacgcccg gcttgacgtt
     1981 cggccagccg atacagaaga tgggcgtgcg cacatcggtg cagcgcgaga tttaccttga
     2041 ggacgtgcgc ctgcccgccg agcacctgct ggggcaggag gggcagggcc tgcgcatcgc
     2101 cctcgccgcc ctggacgtgg ggcgtatcga gatcgcggcg cagggggttg ggctgtcgca
     2161 gggggcgtat gacttcgcgc tgaaccacgc caagggcagg gtgcagttcg ggcaggccat
     2221 aggccagaac caggctatca gcttcatgct ggccgatatg cacgtagcca ccgaggccgc
     2281 ccgcatgctg acctaccggg ccgctatgtc ctacgacgag ggcaagccct tcgccaaaga
     2341 agccgctatg gccaagctac tcgccaccga cacggccatg aaagtcacca ccgacgcggt
     2401 gcaaatcctg gggggcttcg gcgtgaccaa ggccagcccc gccgaacgct tcaagcggga
     2461 cgccaacgtc acgcagatat acgagggcac caaccaggtg cagcgcatgg tcatcgcggg
     2521 acatattatg aagtaacgag tgacgagtaa cgagtaacga gtgatgcggc ttgttgagga
     2581 aggagcgtgg tgggtgactg tcggggttgc gggaccaact cccccattgt cattctgagc
     2641 gtggcgaaga gtctcgtcct ccagc
//
LOCUS       DASRWZ010000813         4870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_203797, whole genome shotgun sequence.
ACCESSION   DASRWZ010000813 DASRWZ010000000
VERSION     DASRWZ010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203797"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VFH60_12895"
     CDS             <1..638
                     /locus_tag="VFH60_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6314725.1"
                     /translation="DGLDQIVDPPAQAEIKAADAMPFAEEDELAVYGLNGWQRGLKGR
                     EVLYTEMFEPTANIAGFTSGYSGPGAKTIVPSKAVVKMDFRLVPRQTPEKMLDLLRAH
                     FDARGFNDIEIVQLAALHPAKTAVDSPLVQVSADVWRDLGEHNVMIAPMTGGSGPVSL
                     ISAGLGVPTVVTGGVAFAESRTHSPNEAIRLADFKTSIRYWGRFFAKLADL"
     gene            complement(1054..2766)
                     /locus_tag="VFH60_12900"
     CDS             complement(1054..2766)
                     /locus_tag="VFH60_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 66 protein"
                     /protein_id="HET6314726.1"
                     /translation="MLTIDDIIPDKVTYRPGETARIRVTVSNDGHMAVGACLTLALTH
                     FVTTVEQLTETVRFEPNARTVVQFAFTPPPVPLRGYGLDATLHDEAGQVLAEGSGALD
                     VLERWSQAPRYGFLSDFAPGQTGVGERCDSLLRYHLNVVQFYDWMWRHYKLLPPSEEF
                     TDGMGRRLSLNAVRAAIEGVQARGGAAMAYGAVYGAEPEFAEEHPELVLYDEAGERVS
                     LAGLFYIMNIASDSPWVPLIVSEFAEAVRVMGFDGIHLDQYGFPKTAVTASGEKVELA
                     EHFPLLIDQARAAVVSVRDDAGVIFNAVENWPIETVAPTSQDAVYIEVWPPDVSYNDL
                     RRLIREGRRLSGGKQVILAAYLSPFLGTGPDTDTQTLSQAEAAALLATATIAASGGSH
                     LLLGERDRVLCDPHYPKYVILRPEFAATMRAYYDFLVRYEEFLISPELEDWEHTSSSG
                     AASFSIRGVPAASSAEVGKVWIIARRKPGVHLVHLINLTDQADVAWNALRTAPETLHN
                     LDLSVDGLPAVRQVLLLVPDVDTGKPLPLTWRQEAGKLHVSVPRLTVWAVIACVLEDG
                     GTQE"
     gene            complement(2800..3882)
                     /locus_tag="VFH60_12905"
     CDS             complement(2800..3882)
                     /locus_tag="VFH60_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET6314727.1"
                     /translation="MHNLYTRSIELILANQAPNGAYVASPNFGEYAYCWFRDGTYIAY
                     SMDLVGEHAGAARFYGWATDMVASRQAQVERAVEAARLDNLPRPDDLLHTRYALDGQA
                     SDGDWPNFQLDGFGTLLWGLAEHVSMSGRPMPGEWRAAVDLLARYLAALWRFPCYDCW
                     EEFGDKIHTSTLAALYGGLNAATALVGDATHAHVAGAIKQFVLDNCVLDGTLCKFVGS
                     EAVDSNLIHVATPYRLLSPGDPVKVATSERIERELRQAGGGVHRYAGDSYYGGGEWVL
                     LTAYLGWYYLERGQVSRARDLLRWIEARAGADCMLPEQVAEHLNYPEMLPVWEERWGP
                     SARPLLWSHAAYLTLSEHLRRAGDVV"
     gene            complement(3949..4782)
                     /locus_tag="VFH60_12910"
     CDS             complement(3949..4782)
                     /locus_tag="VFH60_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6314728.1"
                     /translation="MYARSSPIVSALRYVALFAGAFLMLAPFLYMIGTSFKPHAFVLE
                     LPPRFIPSDPTLENYIRAINSNNFGRYFLNSLLVATSSTVVVVMLSSLLAYAFARWDF
                     PGRQFLFYTMLGTMMIPTLVLIIPQFVLAKNLHLLDSLWGLVVVYSAGTAFNMFLLRG
                     FFEEIPQDLFDAATIDGAGAFRTFWSIALPLARPALAAVAIFSFLGAWDEFTWALTAI
                     NNQDLYTLPIALRLFQQQHGTEWGVVFAASAIAVLPTILVFVIFQRHFIKGITSGAIK
                     G"
BASE COUNT          941 a         1443 c         1574 g          912 t
ORIGIN      
        1 tggacggtct ggaccaaatc gtggacccgc ctgcccaggc tgagataaag gcggccgatg
       61 ctatgccctt tgccgaagag gatgaactcg ctgtctacgg cttgaatggt tggcagcgcg
      121 gcctcaaagg tcgcgaggtg ctctacaccg agatgttcga gccgactgcc aacattgcag
      181 gcttcacaag cggctacagc ggtccaggcg ctaagaccat cgttcctagc aaagccgtgg
      241 tcaagatgga cttccggctt gtgccacgcc agacacctga gaagatgctg gacttgttgc
      301 gcgctcactt cgatgctcgt ggcttcaacg acatagagat agtccaattg gcggcgctcc
      361 atcctgccaa gacggctgtt gatagcccgc tcgtgcaggt cagcgctgac gtgtggcgag
      421 atctggggga gcacaacgta atgattgcac ccatgacggg tggcagcggc ccggtgtcgc
      481 tcatcagcgc tgggcttggg gtgccgaccg ttgtgacggg tggtgttgcc tttgccgaat
      541 cacgaaccca ctcccccaac gaggctatca ggctggctga tttcaagacc tcaatccgct
      601 actggggtcg tttctttgct aagttggccg acttgtagca cggccagatt tctggtattt
      661 ccgctgagac tgtgtgctgt tgactatagc tgctattctc atatgttcat agtttgcaga
      721 tagcccctgg ttcccaattc tttttaggag catcgttggc ctgggcagag aaggcaagca
      781 aggaagatga cccttcacgc tcgccccgtg agacccggtt gccggtatct attaatgatg
      841 tgcaggaggc aggcggttac gcgggccgag gaactggccc gaaaggcaag ctggcacttg
      901 cgcctgccac acttggtcgc gcacacgcga acaaggtgct tactctgccc ctgggataaa
      961 tgtcagttcg cagaatgtga tgaatcgcca ttcaacggat tgcgaggttg ggagggttac
     1021 aagctctacc gctcacgcag acaaccaccg cgattattcc tgtgtcccgc catcctcaag
     1081 aacgcaggcg atcacggccc agaccgtcaa tcggggtacg ctaacgtgta gcttacctgc
     1141 ctcctgtcgc cacgtaaggg gcagcggttt cccggtatct acgtcgggga cgagcagtaa
     1201 cacctggcgc accgcgggca acccgtcaac gctcaggtcg aggttgtgca gcgtctccgg
     1261 agcggtacgc agggcattcc aggcgacgtc cgcctggtcg gtcaggttga tcaggtgcac
     1321 aagatggacg ccgggcttac gcctcgcgat tatccatacc ttacccactt cggcactgct
     1381 tgccgcgggc actcctcgta tggaaaaact cgccgcccct gagcttgagg tgtgctccca
     1441 gtcctctagc tccggcgaaa tgaggaactc ttcataccgc acgaggaagt catagtaggc
     1501 ccgcatcgtt gccgcgaact cggggcgcaa gatcacatac ttcgggtagt gggggtcgca
     1561 gaggacgcgg tcccgctcgc cgagcagcag gtgcgagcct ccactagcgg cgattgtggc
     1621 cgtggccagc agcgcggcgg cctcggcctg tgagagcgtc tgagtgtcag tgtcggggcc
     1681 ggtacccaga aagggcgata ggtaggctgc caggatgact tgcttgcctc cgctcaggcg
     1741 tctgccctct ctgatcagcc gccgcaggtc gttgtagctc acgtcggggg gccacacctc
     1801 gatgtaaacg gcatcctggc tggtaggagc gaccgtttcg atgggccagt tctcgacggc
     1861 gttgaatatg acacccgcat catcgcgtac cgacacgacc gccgcgcggg cctggtcgat
     1921 gagcaacggg aaatgctccg ccagctccac tttctcaccg gaggccgtga cagccgtctt
     1981 tgggaagccg tactggtcga ggtggatgcc gtcgaagccc atcacgcgca cggcctccgc
     2041 gaactcgcta actatgagcg gcacccaggg cgagtcggac gcgatgttca tgatgtagaa
     2101 gagtccggcg aggctgacgc gctctccagc ttcgtcgtaa agcacaagct ctgggtgttc
     2161 ctccgcgaat tcaggctcgg caccgtacac cgcgccgtaa gccatcgcgg ccccgccacg
     2221 cgcctgcacg ccctcaatcg cggcccgcac cgcattgagg gaaagacgcc gccccatgcc
     2281 gtcggtgaat tcctccgatg ggggcaacag cttgtaatgc cgccacatcc agtcgtagaa
     2341 ctgcaccacg ttgaggtggt agcgcaggag ggagtcacaa cgctccccga caccggtctg
     2401 gccgggcgcg aagtccgaca ggaagccgta gcgtggtgcc tgcgaccagc gttcaagcac
     2461 gtccagcgcg ccgctccctt ccgccaacac ttgccctgcc tcgtcgtgaa gggtggcatc
     2521 gaggccgtag ccgcgcaggg gcacaggcgg gggtgtgaag gcgaactgca cgactgtgcg
     2581 ggcattcggc tcgaaccgca ccgtttcggt gagttgctcc acggtcgtga cgaagtgcgt
     2641 gagcgcaagc gtgaggcagg caccgacggc catgtgcccg tcgttgctaa ccgttacgcg
     2701 tatgcgtgcc gtttcaccgg ggcgataggt gactttgtcg ggaataatat cgtcaatcgt
     2761 gagcattgga taacttcagg tcggtgatgg gtgtgggcat tagactacgt cgccggcgcg
     2821 gcgcaggtgc tcggagaggg tcagataagc cgcgtgggac cacaggagag ggcgggcgct
     2881 tggcccccaa cgctcctccc aaacgggcag catctccggg tagttgaggt gttcggcgac
     2941 ctgctccggc agcatgcaat ccgcaccggc gcgcgcctct atccagcgca gcaggtctct
     3001 cgcccgcgag acctgccctc gctcgagata gtaccaccca aggtatgccg tcagcagcac
     3061 ccactcgccg cccccgtagt agctgtcgcc ggcgtaacgg tgcaccccac cgccggcctg
     3121 gcgcaattct cgctcgatgc gctcgcttgt cgcgaccttg acggggtcgc cgggagacag
     3181 caggcggtaa ggggtcgcca cgtgtatcag gttgctatcg accgcctcgc tacccacgaa
     3241 cttgcacagg gtgccgtcga gcacacagtt gtccagcacg aactgcttga tcgccccggc
     3301 gacatgggcg tgagtagcgt ctcccacgag ggcggtggca gcgttcaggc cgccgtagag
     3361 ggcggcgagg gtggaggtgt gtatcttgtc gccgaactcc tcccagcagt cgtagcaagg
     3421 gaaccgccag agggcggcca ggtagcgggc gagcaggtcc acggccgcgc gccactcccc
     3481 aggcatgggg cggcccgaca tggacacgtg ctcggctagg ccccagagga gggtgccgaa
     3541 gccatccaac tggaagttgg gccagtcgcc gtcgcttgcc tgtccgtcga gggcgtagcg
     3601 ggtgtgcagc aggtcgtccg gccggggcag gttgtcgagg cgcgcggctt cgaccgcccg
     3661 ctccacctgg gcctggcggc tggcaaccat gtcggttgcc caaccgtaga agcgggcagc
     3721 gccggcgtgc tcgcccacga ggtccatcga atatgcgatg tacgtgccgt ctcggaacca
     3781 gcaataggcg tattcaccaa agttggggct tgctacgtac gcgccgttgg gggcctggtt
     3841 ggccaggatt agctcgatac ttctggtata taggttatgc atcgtggtgc cgtggactcc
     3901 tgattgcact ttgttcgaca ttgtagttta ccgcaccctt gttgaccgct agccctttat
     3961 cgcgccggaa gtaataccct tgataaagtg gcgttggaaa ataacgaaca cgaggatggt
     4021 cggcagtact gcgatggccg aggcggcgaa caccacaccc cactcagtgc cgtgttgttg
     4081 ctgaaacaag cgcagggcaa tcggcagcgt gtagaggtcc tggttgttga tggcggtcag
     4141 cgcccaggtg aactcgtccc acgcgccgag gaaggagaat atcgccaccg cggccagggc
     4201 cgggcgggcc aggggcaggg cgatcgacca gaaggtccga aacgcccccg cgccgtcaat
     4261 ggtggcggca tcgaacaggt cctggggtat ctcctcgaag aagccgcgca ataggaacat
     4321 gttgaaagcg gtgcccgccg agtagacgac caccagcccc cacaggctgt cgagcaggtg
     4381 gaggttcttg gcgagcacga actgcgggat gatcaggacc agggtgggga tcatcatcgt
     4441 gccgagcatg gtgtagaaaa ggaactggcg gccggggaag tcccagcgag cgaacgcata
     4501 ggccagcagc gaggagagca tcaccactac cacggtggag ctggtagcca cgagcaggct
     4561 gttgaggaag tagcggccga agttgttgct gttgatggca cggatgtagt tttccaaggt
     4621 agggtcactg gggatgaagc gtgggggtag ctcaagcacg aaggcgtgcg gcttgaacga
     4681 cgtgcctatc atgtacagga acggcgcgag catgaggaaa gcccccgcga acagggccac
     4741 gtaacgtagc gcgcttacga ttggcgaaga acgtgcgtac actgcgtaat accacctgtc
     4801 ctttgctgcc tgggataata atttgaccag cgttgcgtgt ctactcacaa tctcggatca
     4861 gacttgtggg
//
LOCUS       DASRWZ010000814         1774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_889401, whole genome shotgun sequence.
ACCESSION   DASRWZ010000814 DASRWZ010000000
VERSION     DASRWZ010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889401"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1774
                     /locus_tag="VFH60_12915"
     CDS             <1..>1774
                     /locus_tag="VFH60_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314729.1"
                     /translation="LGTVLVTALAFAFRQWGAGGHLAPPLDDTFIHFQYARQMAAGYP
                     FQYNTGDPASSGASSFIYPFLLAPAFLLGLDGQKPLLFADLLNFIAHLGSVLLLYKLA
                     FQLGGRPLALFASILLLLDGRLNYIFLSGMETGLFTLALVTFFWLLSREEGAERLVWL
                     AVVGSFAALLRPEGHIIMSVACVLALVLAWRHGRLRARHLWLIVPVLAGLLPYLANLL
                     MTGQLQFNTAASKSIWYLPYAPFYENLSLTAGWAITEVKNTYLGLEIGRSPYPLLALP
                     VAVLGAGVALSGLRYRFLHALLVITFLLGTALALLLPPIHFNRYHMPYDFIFLLYLAI
                     GVIALARVAGRAVGLVRADVGDSGETAVGSRSGSRPVWWAGAAVGAMLLPQFVSYFFA
                     LGDSARDIYSQQMTFSEWVKQYTPADARIGVNDTGAHKYISGRYVIDLIGLTNNRLQG
                     AYFGGWGTIYDRLAAMPEAERPTYLLIHPNVFLNGVDESVAGSLITPMYSIGVQNPII
                     TAGSVETLYKVNWEYALLSEPQTYVLQRDKQPLDMLNVGDVADEKRHGYDISARQSTF
                     SAPKSIVTTAFYEGQEFALSESGRR"
BASE COUNT          274 a          567 c          579 g          354 t
ORIGIN      
        1 gctcggcacg gtgctggtga cagcgctggc gttcgctttt cggcagtggg gggcgggcgg
       61 gcacctggca ccacccctgg acgacacctt catccatttc cagtacgccc gccagatggc
      121 cgccgggtac ccgttccagt acaacaccgg cgatccggct tcttcggggg ccagctcctt
      181 catctatcct ttcctgctcg cccccgcctt tctcctgggg cttgacgggc agaagccgct
      241 gctctttgcc gacctgctca acttcattgc gcacctgggt agcgtgctgc tgctctacaa
      301 gctggcgttc cagctgggcg ggaggccact ggccctcttt gcctcgatac tgctcctgct
      361 cgacggcagg ctgaactaca tcttcctgtc gggaatggag acgggccttt tcacacttgc
      421 gctggtgacc ttcttctggc tgctgtcgag agaggagggc gcggagcggt tggtctggct
      481 ggcggtggtt ggctcgttcg cggcgctgct caggccggag gggcatatca tcatgtcggt
      541 ggcgtgcgtg ctggcgctgg tgctcgcgtg gcggcacggt aggctgcggg caaggcacct
      601 gtggctcatt gtgccggtgc tcgccgggct gctgccgtac ctcgccaacc tgctgatgac
      661 ggggcagttg cagttcaaca ccgccgcctc caaatcgata tggtacctgc cttacgcccc
      721 gttctacgaa aacctttccc tcacggcggg ctgggccatt acggaggtga agaacacgta
      781 cctggggctg gagatcgggc ggtcgccgta cccgctgctg gcgctgccgg tcgccgtatt
      841 gggcgcgggc gtggccttga gcggcttgcg ctaccgcttc ctgcacgcgc tgctggtgat
      901 taccttcctg ctcggcaccg cgctggccct gctgctaccg cccatccact tcaaccgcta
      961 ccacatgccc tacgacttca tcttcctgct ctacctggct ataggggtaa tcgcgctcgc
     1021 gcgcgtggcg gggcgcgcgg tagggctggt aagggctgat gtgggagata gcggggaaac
     1081 ggcggtgggg tcgcgctccg ggtcgaggcc ggtgtggtgg gcgggggctg ccgtgggtgc
     1141 gatgctcctg ccgcagttcg tgagctactt cttcgcgctt ggagacagcg cccgcgatat
     1201 ttactctcag cagatgacct ttagcgagtg ggtcaagcag tacaccccgg ctgacgcccg
     1261 catcggcgtg aacgacacgg gggcgcacaa gtacatcagc ggccgctacg tcatcgacct
     1321 tatcggcctg accaacaacc gtctgcaagg ggcctacttc ggcggctggg gcacgatcta
     1381 cgaccgcctg gcggccatgc ctgaagcgga gcggcccacc tacttgctca tccacccaaa
     1441 cgtcttcctc aacggcgtgg acgagagcgt ggccgggagc ctcattacgc ccatgtactc
     1501 cataggcgtg cagaacccca tcatcacggc ggggagcgtg gagacgcttt acaaggtgaa
     1561 ttgggagtac gcgctcttga gcgagccgca gacttacgtg ctacagcggg acaaacagcc
     1621 cctggacatg ctcaacgtgg gcgacgtggc cgacgagaag cggcatggct acgacatttc
     1681 ggcccggcag tcgaccttct cagcgcccaa gtccatagtc accacggcgt tctacgaagg
     1741 ccaggagttc gccctcagcg agagcggtcg cagg
//
LOCUS       DASRWZ010000815         6719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1300440, whole genome shotgun sequence.
ACCESSION   DASRWZ010000815 DASRWZ010000000
VERSION     DASRWZ010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300440"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VFH60_12920"
     CDS             <1..1249
                     /locus_tag="VFH60_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6314730.1"
                     /translation="SFERATDPRLAGSLPPTQLPAGLFLNDIVGVMDKLEGKATEIEG
                     VKALDPTTLEITIDEAKGYFLSKLTSGPAFVVQRANVEEGPRWTEHPRGTGPFRLEKW
                     VHNDQMVLVANNLYYGGRPALSTINIWMGANATGELQQYEVGGLEVANVPVDDLYRVT
                     DRNNPMSKELQSVPELSVTYLGFNLRHKPFDDPKIREAFALAIDRQKIARVMFQSRVQ
                     QASGFVPPHMAGYAPPEAELGYDVTRARQLIGESTYKEVKNLPRVRLYTAGEALGPML
                     QEVYSQTLGIDVEVHEVEWSDYLDGLARHDYPMFTLTWSADFPDPEAILGSLFRSTSP
                     ENHTGYRNADVDAVLSVASTEKDRQSRMNVYREVERRILKDLPAVPLYHSVSYTLVKP
                     YVTGLNVTPMGILSLKDVRLAPR"
     gene            1258..2145
                     /locus_tag="VFH60_12925"
     CDS             1258..2145
                     /locus_tag="VFH60_12925"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6314731.1"
                     /translation="MQSLMSALFYRGNARSDEQPEIEPLTPATVRDLRLPWLSRFNSS
                     LLTHHLQQHPGHALWVPRTGEYVVWEPWRRRDDIASVMEVTARRGRDALIEEMAATVR
                     EEGYGLLLCADEVWRDHTKSWLGSGFAQVERIVFFRRDLRDGMAGLDPSLLPKLTYRR
                     ADLSYMDDLLAVDHDSFPWMWWNSYAEFEMYLQMSSVHAYMAYLDNMPVGYASFTMYD
                     GWAHLDRLSVASAYQGRRLGAAQLMHALHRMRELGAASVGLSTQEDNTQSHRLYRSFG
                     FKQQSDTMTFYGRELGRDE"
     gene            2310..3563
                     /locus_tag="VFH60_12930"
     CDS             2310..3563
                     /locus_tag="VFH60_12930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6314732.1"
                     /translation="MKVLMLSWEYTPHIVGGLGKHVVELVPELIADGVEVHLVVPRLG
                     GGEDSEPLPLPDGSPAANGSRVYRVPPANAQGDFFAKTFYDNAQVRAFASRLVAQEGG
                     FDLIHNHDWLSSFAAIDLKYDFRIPLLTTIHATEMGRNQGNLYGGLQQAIHQAEWRTS
                     YESWRIIACSHYMAWEIQTYFGVPPEKIDVIPNGVDPRRFDALNGVDLSEFRLNFARA
                     DQPIVYYVGRMVPEKGLAVLVESVPYVLREWPGVKFVLAGGGGYVEELRAKARALGVG
                     DNIIFPGRISDEDRDGLFKVANAAVFPSLYEPFGIVALEAMAAGTPVIVSEVGGLTEV
                     VDLHRTGITVKPDDPQSLAWGILHTLKNPEWSATRASNAHQAVRAEFNWPRIADLTTR
                     VYHRVVDEACGGDWAYKVPEAAAAT"
     gene            3620..4240
                     /gene="dcd"
                     /locus_tag="VFH60_12935"
     CDS             3620..4240
                     /gene="dcd"
                     /locus_tag="VFH60_12935"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET6314733.1"
                     /translation="MLLSDKKIMEGMEQGNIVIEPFEPRQLGTNSYDVRLGPYYFIPN
                     HNMVTVNFTEEEDAKSFWLGPYFAEDGLIRIRPGDTILAHTEEVVGGRNGFTTSMRSR
                     SSIGRSCLSVCKCAGVGDVGYVSRWTMEITNHSHAMAIVPVGLRVAQILFYEVGETAR
                     EYQGKYGSWNDWSPKDMLPKLYRDYDLQRVGTNRVPARNGHETLAR"
     gene            4700..5068
                     /locus_tag="VFH60_12940"
     CDS             4700..5068
                     /locus_tag="VFH60_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314734.1"
                     /translation="MCGRHFCAQHGDVDASVCRLCSASYRRKLHDEASQVAEIERRVS
                     AEARNNDGICGWQDCPNELYARCERCGASYCPRHISRHRYHYRYRTRKGIYKRQAEVT
                     LCDACKPYLDEYKTEKSWRD"
     gene            5143..6711
                     /gene="gpmI"
                     /locus_tag="VFH60_12945"
     CDS             5143..6711
                     /gene="gpmI"
                     /locus_tag="VFH60_12945"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET6314735.1"
                     /translation="MSNTDAGRPRPVVLVILDGWGLREETENNAVALADTPNMDRLWA
                     GSPHTALDASEHAVGLPSGQMGNSEVGHQNMGAGFIVYQELTRLDKSIEDGSFYENPE
                     LVAACEHVKARGSTLHLLGLLGPGGVHSHWAHLFALLELAKRHGLERVVYHAFTDGRD
                     TPPQSGLGYMQVVLEKMRETGVGRVGSVSGRYYAMDRDKRWERVEKAYDALVHGQGRR
                     FADPLEVFAASYAEGVTDEFIVPAVIAAEGEDPSTIRDGDGVIFFNFRSDRGRELTQA
                     ITDADFQGFDRGQALTDLYYVTMTRYAAELNVHVAFQAMEVKVPLARVLSDLGLRQFH
                     IAETEKYAHVTFFFNGRTEPPFPGEDRELVPSPKVATYDLQPEMSAYGIAEELRERIA
                     SGKYDFIVANFANGDMVGHSGKLDATIAACEAVDRCVGQVFQAVREAEGVMLITADHG
                     NAEMMADPDGGGPHTFHTTNPVPFILAAPDDSPLRNATLRNGGRLCDLSLTILDIMGI
                     DAPGDMNCRTLIHH"
BASE COUNT         1308 a         2048 c         2212 g         1151 t
ORIGIN      
        1 ctcgttcgag cgggccaccg accccaggtt ggccgggtcg ctgccgccca cacagttacc
       61 cgccgggctg ttcctaaacg atatcgtggg cgtgatggac aaactcgaag ggaaggcgac
      121 cgaaatcgag ggcgtgaagg ccctggaccc caccacgttg gaaatcacga ttgatgaagc
      181 aaagggctat tttctgtcga agctgacttc cggcccggcg ttcgtggtcc agcgcgccaa
      241 cgtggaggaa ggtcccaggt ggaccgagca ccctcgcggc acggggccgt ttcgcctgga
      301 gaagtgggtg cacaacgacc agatggtgct ggtcgccaac aacctgtact acggtggacg
      361 ccccgcgctc tctaccatca acatctggat gggcgctaat gccacgggcg aattgcagca
      421 gtacgaggtg ggcgggctgg aagtggcgaa cgtgccggtg gacgacctct accgcgtgac
      481 cgaccgcaac aaccccatgt ctaaggagct acagtcggtg ccggagttgt cggtgaccta
      541 cctgggcttc aacctgcgac ataagccctt tgacgacccc aagatacggg aggccttcgc
      601 cctggctatc gaccggcaga agatagccag ggtgatgttc cagtcgcgag tgcagcaggc
      661 cagcgggttc gtgccgccgc acatggcggg gtacgccccg cccgaagcgg aactgggtta
      721 cgacgtgaca cgcgcgcgcc agttgatagg ggagtctacc tacaaagagg taaagaacct
      781 gccgagggtg cgcctctaca cggcggggga ggcgctcggc cccatgctgc aagaggtgta
      841 cagccagacg ctggggatcg acgtcgaggt gcacgaggtg gagtggagcg actatctcga
      901 tgggctggcc cggcacgact acccgatgtt cacgctgacg tggagcgccg atttccccga
      961 ccctgaggcc atactgggca gtctcttccg ctccacctcg cccgagaacc acaccggcta
     1021 ccgcaacgcc gacgtggacg ccgtgctcag cgtcgccagc accgaaaagg accgccagag
     1081 ccgcatgaac gtctacaggg aggtggagag gcgtattttg aaggacctcc cggcggtgcc
     1141 gctgtaccac tctgtttctt acacgctggt caagccatac gtcacggggc taaacgtcac
     1201 gccgatgggc attttgagcc tcaaagacgt gcgcctggcc ccgcgctgag gagggcattg
     1261 cagagcctca tgtccgcgct cttctaccga gggaacgcca ggagcgacga gcagcctgaa
     1321 atagagccgc tgaccccggc cacggtgcgc gacctgcgcc tgccgtggct cagccgcttc
     1381 aactcgtcgc tcctcaccca ccacctccag cagcacccag gccacgccct gtgggtgccg
     1441 cgcacgggtg agtacgtagt gtgggagccg tggcggaggc gcgacgacat cgccagcgtg
     1501 atggaagtca cggcgaggcg gggccgcgat gcgctgatag aggagatggc cgccacggtg
     1561 cgagaagagg gctacggcct gctgctgtgc gcggacgagg tatggcggga ccacacgaag
     1621 tcgtggctgg gcagcggttt cgcccaggta gagcggatcg tcttcttccg gcgcgatttg
     1681 cgggacggta tggccgggct ggacccttcg ttgctgccca agctgacgta tcgccgcgcc
     1741 gatctctcct acatggacga cctgctcgcc gtggaccacg actcgttccc ctggatgtgg
     1801 tggaacagct atgccgagtt cgagatgtac ttgcagatga gcagcgtcca cgcgtacatg
     1861 gcttacctcg ataacatgcc agtggggtac gcctcgttca ccatgtacga cggctgggcg
     1921 cacctcgacc ggctctcggt ggcctccgcg taccagggcc gccgtttggg tgccgcgcaa
     1981 ctgatgcacg ccctgcaccg catgcgggag ctgggggccg cctcggttgg cctttccacc
     2041 caggaggaca acacccagtc ccatcgcctt tacaggtcct tcggcttcaa gcaacaatcc
     2101 gacacgatga cgttctacgg gcgggaactg ggacgtgacg agtaacacgt aacgcgtaac
     2161 acgtaaagag acgaaaccgc aaaggcgcaa agcgcgcaaa gaacgcgcaa aggggatttc
     2221 ggcttggatg cctgtcgctt ggcactcatg cctggtcact cgtcgcgcaa atccgaaatt
     2281 cgcaattcga aatcccaaat ggaggatcta tgaaagtcct gatgctgtcg tgggagtaca
     2341 cgccgcacat tgttggcggg ctgggcaagc acgttgtgga gcttgtgccg gagctaatag
     2401 cggatggggt ggaggtgcac ctggtggtgc ccaggctggg cggtggggaa gatagcgaac
     2461 ctctgccgct ccccgatgga agtcccgccg cgaacggcag ccgcgtctac agggtgccac
     2521 ctgctaacgc gcaaggggac ttcttcgcca agaccttcta cgacaacgca caggtccgcg
     2581 ctttcgcctc tcggttggtg gcccaggagg gcggcttcga ccttattcac aaccacgact
     2641 ggctcagcag cttcgcggcc atagacctga aatacgattt ccggatacct ctgctcacga
     2701 ccatccacgc caccgagatg gggcgcaacc aaggcaacct ctatggcggc ttgcaacagg
     2761 ccatccacca ggccgagtgg cgcacttcgt acgagtcgtg gcgcatcatt gcgtgcagcc
     2821 actacatggc ctgggaaatc cagacctact tcggcgtgcc cccggagaag attgacgtaa
     2881 tacccaacgg cgtggaccct cgccggttcg atgcgctgaa cggcgtcgat ttgtcggagt
     2941 tccggctcaa tttcgcgcgt gccgaccagc cgattgtgta ctacgtgggg cgcatggtgc
     3001 ctgagaaagg gcttgcggtg cttgtcgagt cggtgcccta tgtgttgcga gagtggccgg
     3061 gtgtgaagtt cgtgctggcg gggggcggtg gatatgtcga ggaactgcgt gcaaaagccc
     3121 gcgccctggg agttggggac aatataatct tccccggacg catatccgac gaggatcgcg
     3181 acgggctgtt caaggtagcc aacgcggcgg tcttcccaag cctgtacgag ccgttcggca
     3241 tagtggcgtt ggaggcgatg gcggcgggca ccccggtgat cgtgagtgag gtgggcgggc
     3301 tgaccgaggt ggtggacctg caccgcaccg gcatcacggt aaagccggac gacccgcaat
     3361 cgctggcctg gggcatcctg cacacgctga agaacccgga gtggtcggcc acgcgggcct
     3421 cgaacgccca ccaggcggtg cgcgcggagt tcaactggcc gcgcatagcc gacctgacga
     3481 cgagggtgta ccacagggta gttgacgaag cctgcggggg cgactgggct tacaaggtgc
     3541 ccgaagcggc cgccgcgaca tagagactcg gcctggtagc agacgaaagg ccggggccac
     3601 gagcaagagg agcaaaacag tgctgctatc agacaagaag atcatggaag gcatggagca
     3661 ggggaatatc gtcatcgagc cgttcgagcc tcggcagctt ggcaccaact cctacgacgt
     3721 gaggctcggc ccctactatt tcatcccgaa ccacaacatg gtgacggtga acttcaccga
     3781 ggaagaggac gccaagtcgt tctggctagg cccctacttc gcggaggatg gcctcatccg
     3841 catccgcccc ggcgatacga ttttggccca cacggaggaa gtggtaggcg gccgcaacgg
     3901 cttcaccacg tccatgcgct cgcggtccag catcgggcgg tcgtgcctgt cagtgtgcaa
     3961 gtgcgcgggg gtaggcgatg tgggttacgt ctcgcgctgg acgatggaga tcaccaacca
     4021 cagccacgcg atggctatcg tgccggttgg cctgcgggtc gcccaaatcc tgttctacga
     4081 ggtaggcgag acggcgcgcg agtaccaggg caagtacggc tcgtggaacg actggtcgcc
     4141 caaggacatg ctgcccaagc tgtaccgcga ctacgatctt cagcgcgtgg gcaccaaccg
     4201 tgtccccgcc cgcaacgggc acgagacgct ggctagatag cgacgtgtcg ggacgagtga
     4261 cgcggaacgc gtaaggcacg cttgcgacgg gagccgggcc gggttggcct gcatggacgc
     4321 cctgtggctg aagccacggg ctgggaagta gcgaagtctc cttcggagac tacttagtgc
     4381 tacagagtcc ccgcagggga ctttgcctct tctcagcccg tggctttagc cacagggcgt
     4441 ccccaatgta cattgtctcg cttgactgcc tcacgccgtg gcagaggatc cgtgttgcga
     4501 aacagcgggg aattaggcat actacattgt tggtttggac cgtttcgccg atgtggcagt
     4561 agtggtccca aaatgggtag ttaccctagt tttccgaatt acgcgttacg cgttacgcct
     4621 cacgagaagc tgacttatgc cattacgctg tgaaagcccc attacaattt gctttcggac
     4681 tccggcggcc cagtgcatca tgtgcggccg ccacttttgc gctcaacatg gcgatgtaga
     4741 cgcctcggtg tgcaggctgt gcagcgcctc ctaccgtcgc aagctgcacg acgaagcctc
     4801 ccaggttgcc gagatagagc gccgcgtgag cgcggaggcc cgcaacaacg acggtatttg
     4861 cggctggcag gactgcccga acgagctata cgcccggtgc gagcgttgcg gggcctccta
     4921 ctgtccccgc cacatcagcc gccataggta tcactatcgc taccgcacgc gcaagggtat
     4981 ttacaagcgg caggccgagg tcacgctctg tgacgcctgc aaaccctacc tcgatgagta
     5041 caagaccgag aaaagctggc gcgactgagc cgctcgcgca gatggcgtga agccggtaga
     5101 gagcgggcac cctgcaacat acacaaccac aggtggacgc gaatgagcaa taccgacgcc
     5161 ggtaggcccc ggcccgtggt cctggtgatc ctcgacggct ggggcttgcg cgaggagacg
     5221 gagaacaacg ccgtcgcgct cgcggatact ccgaacatgg accgcctttg ggccggctct
     5281 ccccacacag ccctcgatgc ttccgaacac gcggtgggcc tgccttcggg gcagatgggc
     5341 aactcggaag tggggcacca gaacatgggc gcgggcttca tcgtgtacca ggagcttacc
     5401 cgcctcgaca agtctatcga ggacggctcg ttctacgaga accccgaact cgtggcggcc
     5461 tgcgagcacg tgaaggcgcg cggctccacg ttgcacctgc tggggctgct ggggccgggc
     5521 ggggtgcata gccactgggc gcacctgttc gcgctgctgg aactcgcgaa gcggcacggg
     5581 ctggagaggg tggtctatca cgcctttacc gacgggcgcg acacacctcc ccagagcggc
     5641 cttggctata tgcaggtggt gctggagaag atgcgcgaga cgggcgtggg ccgcgtgggt
     5701 agcgtgtcgg gccgctacta cgcgatggac cgggacaagc ggtgggaacg ggtagagaag
     5761 gcatacgatg cgctcgtgca tgggcagggc aggcgctttg ccgatccgct tgaggtgttc
     5821 gccgcctcct acgcggaggg agtcacggac gagtttatcg tgcctgccgt tatcgctgcc
     5881 gagggtgaag acccgagcac aatacgggac ggcgacgggg tgatcttctt caacttccgc
     5941 agcgaccggg gccgggagct tacgcaagcc atcaccgacg ctgatttcca ggggttcgac
     6001 cgggggcagg cgctcaccga cctgtattac gtcaccatga cgcgttacgc ggcggagctt
     6061 aacgtgcacg tcgcgttcca ggcgatggag gtgaaggtac cgctcgcccg cgtgctgagc
     6121 gacctcggcc tgcggcaatt tcacatcgcg gagaccgaga agtacgccca cgtgaccttt
     6181 ttcttcaacg gccgcaccga gccgcccttt ccgggggagg accgggaact ggtgccgtcg
     6241 ccaaaggtgg cgacctacga tcttcagcct gaaatgagcg cgtacggcat agcggaggag
     6301 ctacgggagc gcatcgcgag cgggaagtac gacttcatcg tggcgaactt cgctaacggg
     6361 gacatggtgg ggcatagcgg caagctggac gccacaatcg cggcttgcga ggcggtggac
     6421 cgctgcgtgg ggcaggtgtt ccaggccgtg cgggaggcgg agggtgtgat gctgatcacg
     6481 gccgatcatg gcaacgccga gatgatggcc gacccggacg gtggcggccc gcacaccttc
     6541 catacgacca accccgtacc cttcatcctg gccgcgcccg acgacagccc cctccgcaac
     6601 gccacgctaa ggaacggcgg ccgcctgtgc gatctcagcc tcaccatcct tgacataatg
     6661 ggaatcgacg cgcccggcga catgaactgc cgcacgctca tccaccacta gacaccccg
//
LOCUS       DASRWZ010000816         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_135227, whole genome shotgun sequence.
ACCESSION   DASRWZ010000816 DASRWZ010000000
VERSION     DASRWZ010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135227"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /gene="radA"
                     /locus_tag="VFH60_12950"
     CDS             <1..1486
                     /gene="radA"
                     /locus_tag="VFH60_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET6314736.1"
                     /translation="AEVKRFLSEIGIMGREEAVSHIAALSEPGLPSTQRDTIPTGKAF
                     WRHLQQVKGDLTYAELTRLAGTKVKNRRHESPLCRATVSKLATVLDDPPLKALADGDV
                     YWDEIASVTPAGEAPVYDISVPSKENFVANDLIVHNSTLLLQVSGELALWGDGAILYV
                     SGEESPQQIKLRASRLGINPERLMLLSETDLDSVLGQIETLQPELVIIDSIQTMFVPE
                     LTSAAGSVSQVRECTARLTRLAKQRNIPMFLVGHVTKEGALAGPRVLEHMVDAVLYLE
                     GDRFHQYRLLRGVKNRFGSTNEVGVFEMSNEGMIEVTNPSAAFLAERSEGAPGAAVAV
                     TLEGTRPILVEVQALTSSTSFGMPRRTANGIDMNRLLVLTAVLTKRVGLGLSAQDIYV
                     NIVGGLKISEPAVDLGVAVAIASSFRDVQVSPDVVCIGEVGLSGELRSVGQLERRLLE
                     AAKLGFTRAIYPPIVGKGKLVLPKGLQGRSVATLQEAIESALQK"
     gene            1513..2289
                     /locus_tag="VFH60_12955"
     CDS             1513..2289
                     /locus_tag="VFH60_12955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6314737.1"
                     /translation="MAQGTAEDNPRWQETDSETFVEYGSYFIPEREVQTDVICSLIPA
                     ASGECRMLDLCCGEGVLTYALLERFPGSHVFGYDGSQTMLDTARAALSIFAERFETRL
                     FDLADTSWRSFPQPLHAVVSSLAILHLDAEQKQRLFRDVYAMLEPGGAFVIADIIQPT
                     SKQAVDLAGRQWDEAVRQRSLHLSGDLAAYEEFHTLGWNSFTAPEPDPVDKMSLLFDQ
                     LKWLEHAGFEGIDVFWAKAGHAVFGGYKPRGGRPVAKATG"
     gene            2423..3460
                     /locus_tag="VFH60_12960"
     CDS             2423..3460
                     /locus_tag="VFH60_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET6314738.1"
                     /translation="MGQTSTNRYSRQTNLPQIRREGQEKLAAATVLVSGVGALGSSMA
                     EMLARAGVGTLRLADRDYLELHNLQRQSLYTEADVEGGLPKAVAAARHIGQINSEVRV
                     EPCVVDITPETVGDLLDGVTVALDGTDNLQTRYLLNDACIELGIPWVYSGVIGVSGVS
                     LSVVPGKTACLRCVFPDPAPPGTLQTCDVAGVLGPAAHAFASIACAEAIKLLVGAPPA
                     PGMLTADLWEGSWDRFDMPRNPHCPTCGLRRFDFLHGEAWGPQDAARLCGREAIQVRV
                     GSRGDRLDLHTRGERLGAAGAGEVLANEYVVRLRTPHYELTIFPDSRVIVKGTEDAAV
                     ARGLVAKYVGT"
BASE COUNT          626 a         1054 c         1232 g          623 t
ORIGIN      
        1 tgccgaagtg aagcggttcc tttcggagat aggcataatg ggtcgcgaag aggcagtatc
       61 gcacattgct gcgctgtccg aacccggctt gccctctacg cagcgcgata ccatacctac
      121 aggcaaagct ttctggcggc acctgcaaca ggtgaagggc gatctcacgt acgcggagct
      181 gacccggctg gcgggtacca aggtaaagaa ccgcaggcac gaaagcccgc tttgccgggc
      241 aacggtgagt aagcttgcaa ccgtgcttga tgaccccccg ctgaaggccc tggcggacgg
      301 cgacgtttac tgggacgaga tcgcaagcgt taccccggcg ggcgaggccc cggtctacga
      361 catcagcgtg cccagcaagg agaactttgt cgccaacgac ctgatagtgc acaactccac
      421 ccttctgctg caagtaagcg gcgagcttgc gctctggggc gatggggcta tcctgtacgt
      481 gtcgggcgag gaaagccccc agcagataaa gctgcgcgcc tcgcggttgg gcatcaaccc
      541 tgagcggctc atgctgctgt cggagaccga cctcgactct gtgctggggc agatagagac
      601 gctgcaaccg gagcttgtga ttatcgactc gatacagacg atgttcgtgc ccgagctcac
      661 ctcggcggcg ggttcggtga gccaggtgcg cgagtgcacg gcccggctga cgcgcctcgc
      721 caagcagcgt aacatcccca tgttcctggt gggccacgtc accaaggagg gcgcgctggc
      781 cggaccccgc gtgctagagc acatggtgga cgccgtgctc tacctcgagg gcgacaggtt
      841 ccaccagtac cgcctgctgc ggggggtgaa gaaccgcttt ggctccacca acgaggtagg
      901 cgtgttcgag atgagcaacg agggcatgat agaagtgacc aacccctcgg cggcgttcct
      961 ggcggagcgg agcgaggggg cgccgggcgc tgccgtggcg gtcacgttgg agggcacgcg
     1021 ccctatcctg gtggaggtgc aggcgctgac ctcctctacc tccttcggca tgccccgccg
     1081 caccgccaac ggcatcgaca tgaatcgtct gctggtgctc accgccgtgc tcaccaaaag
     1141 ggtggggctt ggtctttcag cgcaggacat ctacgtgaac atcgtgggcg gcctcaagat
     1201 tagcgagccg gcggtggacc tgggcgtggc ggtggctatc gcgtcgtcgt ttcgggacgt
     1261 gcaggtgtcg cccgacgtgg tgtgcatagg cgaggtgggg ctgtcgggcg agctacgcag
     1321 cgtggggcag ttggagcgcc ggttgctcga ggcggccaag ctaggcttca cccgcgctat
     1381 ctacccgccc atcgtgggca agggcaagct cgtgctgccg aagggcttgc agggcaggtc
     1441 ggtagccacg ctgcaagagg cgattgagtc ggcgttgcaa aagtagaggt agaggcccac
     1501 atggagggaa cgatggcaca ggggaccgcc gaagataatc cgcgctggca ggagaccgac
     1561 tccgagacgt tcgtggagta cggcagctac ttcatacccg agcgtgaggt gcagaccgac
     1621 gtgatttgca gcctcatacc ggcggcgtcc ggggagtgcc gcatgctcga cctgtgttgt
     1681 ggagagggag tgctcaccta cgcgctgctg gaacgcttcc caggctcgca cgtcttcggt
     1741 tatgacggct cgcagacgat gctggacacg gcgagggcgg cgctatctat cttcgcggag
     1801 cggttcgaga cgcggctgtt cgacctggcg gatacctcct ggcgcagctt tccgcagccc
     1861 ctgcatgcgg tagtctcctc cctcgccatt ctccacctcg atgccgagca gaagcagcgg
     1921 cttttccgcg acgtgtacgc catgttggag ccgggcgggg cgttcgtcat agccgacatc
     1981 atccagccca cttccaaaca ggccgttgat ctcgcgggca ggcagtggga cgaggcggtg
     2041 cgccagcgtt cgctccacct cagcggcgac ctggcggctt acgaggagtt ccacacgctg
     2101 ggctggaact cgttcactgc ccctgagccg gacccggtgg acaagatgtc gctgttgttc
     2161 gaccagctaa aatggttgga gcacgcgggc ttcgagggca ttgacgtgtt ctgggcgaag
     2221 gcgggtcatg cggtgttcgg ggggtacaag ccccgcgggg gacgccctgt ggctaaagcc
     2281 acgggctgag aagcggcaaa gtcccctacg gggactctct tggagagggt agtctccgta
     2341 ggagacttcg tctctggtaa gcccgccaat tcattggcag ggcgatggtg gcagggcgtg
     2401 acgtaggaca atacaggtga ctatgggcca aacctccaca aacaggtact cacgccagac
     2461 caaccttcct caaatccgcc gcgagggaca ggagaagctt gccgcggcca ccgtgctggt
     2521 ctcaggggtg ggggcgcttg gctcgtctat ggccgagatg ctggctaggg cgggcgtggg
     2581 gacgctacgg ctggcagacc gcgactacct ggagctgcac aacctccaac gccagtcgct
     2641 ttataccgag gcggacgtgg agggcgggct gcccaaggca gtggccgctg cccggcatat
     2701 cgggcaaatc aactcggaag tgcgcgttga gccttgcgtg gtggacatta caccggagac
     2761 ggtgggcgac ctgctggacg gggtgacggt cgcgctggac ggcaccgaca acctccagac
     2821 ccgctacctg ctcaacgacg cctgcatcga gctcggcatc ccgtgggtct attcgggcgt
     2881 gataggggtc agcggagtga gcctgagcgt ggtgccgggc aagaccgcct gcctgcgctg
     2941 cgtctttcca gaccccgcgc cgcccggcac gctgcaaacg tgcgacgttg ccggggtgct
     3001 cggtcccgcc gctcatgctt tcgcctccat agcctgcgcc gaagcgataa agctccttgt
     3061 aggcgcgcct cctgctcctg gcatgctcac cgcggacctg tgggagggta gctgggaccg
     3121 tttcgacatg ccccgcaacc cccattgtcc cacctgcggc ctgcggaggt tcgacttcct
     3181 gcacggcgag gcgtgggggc cgcaggatgc cgctcgcctg tgcgggcgcg aggctatcca
     3241 ggtgcgggta ggctctcgtg gcgaccggct ggacctccac acgcgggggg agcgcctggg
     3301 ggcagcaggg gcgggagagg tgctcgccaa cgagtacgtg gtccgtctgc gcacgccgca
     3361 ctacgagcta accatcttcc ccgacagccg cgtaatcgtc aaaggtaccg aagatgcggc
     3421 ggtagctcgt gggttggtgg cgaagtacgt ggggacgtga cgagtgacga gtgacgagtg
     3481 acgagtgacg agtgacgagt ggctagtggt tagtgggtag tggttagtgg ttagt
//
LOCUS       DASRWZ010000817         5159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_546642, whole genome shotgun sequence.
ACCESSION   DASRWZ010000817 DASRWZ010000000
VERSION     DASRWZ010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546642"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="VFH60_12965"
     CDS             complement(<1..1717)
                     /locus_tag="VFH60_12965"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET6314739.1"
                     /translation="MIIVPREICADLDRALAKEWLVANGIGGYASSTVAGANTRRYHG
                     LLVAALKPPVERTVLLSKIDEEATIGGRTFYLGTNEYPDGQVLPEGFAHIEGFSIRDG
                     IPTTAFRLGSALLHKTVWMEHGQNTTYIRYTYVEGKAPCELVLHAMCNYRDYHDSTTG
                     SPERDFGVEALPGGCMVVARPGATPFWLSSQPGVDFTRTGGWLWNFVYRGEVERGYDD
                     TDDLYTPGFFRAVLQPGQSLTLVASTEPPEVTSSQVGEAFQRASARARGLYKAAGLKR
                     GDENGDAADPDAPVEAMIAQLVLAADSFIVTRKVSRGGETTQTPSVLAGYHWFTDWGR
                     DTMISLPGLTLPTGRGREAGKVLQTFGIFARDGLIPNHFPDSGAAPHYNTADGTLWMF
                     AATEALAQGRGNISVARNLYPLLADIVAWHIRGTHFGIKVDPEDGLLTVGEAGVQLTW
                     MDAKVDDWVVTERRGKPVEINALWYNALRVMDKLRVSLGRTVVRGGKEELPDFHALAN
                     QVRDSFRTRFWYGAGDHLFDVIDGPGGHDTSIRPNQIFAVSLRDDLLSVDQQRLVLEC
                     VRKHLL"
     gene            complement(1818..2567)
                     /locus_tag="VFH60_12970"
     CDS             complement(1818..2567)
                     /locus_tag="VFH60_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6314740.1"
                     /translation="MLKGVLFDMGGTLLEYRREDWEALDVQLNSDLHTYIASRGHADK
                     LPPLDEFLEVFNARFRSGWEQAARTQKSFNMLNLLDELFSERGIPERAGVDHMVPWYK
                     GVSDMTYVRPDVQPTLERLRDSGLKLGLVSNTAWPSTIHDPDLERFGLLDLLECRVYS
                     CELGWEKPAPPIFRAALDCIGLEPGETAFVGDFRRYDIAGAHAVGMKGIWKRIEGRPD
                     DVDDPSIVPDATITTIGELPEALSVLYGFGI"
     gene            complement(2733..3587)
                     /locus_tag="VFH60_12975"
     CDS             complement(2733..3587)
                     /locus_tag="VFH60_12975"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6314741.1"
                     /translation="MRYTITMDMPANVSAKSTGPLEHIAAPLAPKPGPYPLMPGAEPF
                     LYEAGPVGCLMLHGFTSTPYEMRGLGRFLADRGITAGAALLAGHGTAPEDLANTTWHD
                     WLASVNGALDAMLARCEKVYLAGLSLGGALTLYTAAHRGQDLAGIITMGAPIYVPPPI
                     GSLLRGIQGQMPYLNQPYRDIEDPLAHSRHVSYERSPSAATASLIDFISQVRGVLGQV
                     TVPALVMYARRDHVVPYMNSHHIYSRLASHDKRLVALQRGFHIMTVDTDREKVQQAVY
                     EFIQEREE"
     gene            4003..4437
                     /locus_tag="VFH60_12980"
     CDS             4003..4437
                     /locus_tag="VFH60_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6314742.1"
                     /translation="MTHEWHRDDYTISTDPKRLDLEVIHGFLANDSYWAQGRSVETIR
                     RSIEHSLAFGVYQGERQVGFCRVVTDYATLAWLADVFIVEAYRGRGLAVWLVDTVISH
                     PELQGLKRWLLATKDAHGLYAKYGFGPLTEVERWMTHLGGGR"
     gene            4453..4962
                     /locus_tag="VFH60_12985"
     CDS             4453..4962
                     /locus_tag="VFH60_12985"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HET6314743.1"
                     /translation="MPLELHPHDPRWAAYFDEVKASLSGVLGRYAPDIQHVGSTSIAG
                     IVAKPVIDVAVAIEQYPLPDEVLAAVEALGYTYWGDYGIPHRHLFFQRGGPVGYNIHI
                     NELANEQFQRHVLFRDYLRAHPDAAQEYERLKLELAAQHEDVNTYANSKSEFVRDILK
                     KAHLWREGR"
BASE COUNT          955 a         1621 c         1639 g          944 t
ORIGIN      
        1 tgagcaggtg cttgcgcacg cactccaaca cgaggcgctg ctggtcaacg gagagcaggt
       61 cgtcgcgtag cgagacggcg aagatttgat tgggacggat ggaggtgtcg tggccgcctg
      121 ggccgtctat cacgtcgaac aggtgatcgc ccgcgccgta ccagaagcgg gtgcgaaacg
      181 aatcgcgcac ctggttggca agggcgtgga agtcgggcaa ctcctccttg ccgcccctca
      241 cgaccgtgcg gcccagcgat acccgcagct tgtccatgac gcgcagggcg ttgtaccaga
      301 gggcgttgat ctccacgggc ttaccccgcc gctccgtcac cacccagtca tccaccttgg
      361 cgtccatcca ggtaagctgt acgcccgcct ctcccaccgt gagcaagcca tcctccgggt
      421 ctaccttgat gccgaagtgc gtgccccgta tgtgccaggc cacaatatcg gccagcagcg
      481 ggtagaggtt gcgggccacc gagatgttgc ccctgccctg ggcgagcgcc tccgtcgccg
      541 cgaacatcca gagggtgccg tcggcggtgt tgtagtgcgg ggccgcgccg gagtcgggga
      601 agtggttggg gatgaggccg tcacgggcga atatgccaaa ggtctgtagc actttacccg
      661 cctcgcggcc ccgcccggtg gggagagtaa gccccggcag tgagatcatg gtgtcgcggc
      721 cccagtcggt gaaccagtgg tagcccgcga gcacgctggg cgtctgggtc gtctcgcctc
      781 ctcggctcac tttgcgcgtg acgatgaagc tatcggcggc cagcaccaac tgggcgatca
      841 tggcttcaac cggcgcgtcg gggtcggcgg catcgccatt ttcgtcgccg cgcttcagcc
      901 ccgccgcctt gtacagcccc ctggccctcg cgctcgcccg ctggaaagcc tcgcccacct
      961 gcgatgacgt gacctccggc ggctcggtgc tggccacgag cgtgaggctt tgccccggtt
     1021 ggagcacggc gcggaagaag ccgggcgtgt aaaggtcgtc cgtatcgtcg tagcctcgct
     1081 ccacttcgcc ccggtagacg aagttccaca gccagccgcc ggtgcgagtg aaatcgaccc
     1141 caggttggct tgaaagccag aacggcgtag caccagggcg ggcgaccacc atgcagccac
     1201 ccggcagtgc ctccacacca aagtcgcgct ccggcgagcc ggtcgtgctg tcgtggtagt
     1261 cgcgatagtt gcacatcgcg tgcagcacca actcgcacgg cgctttaccc tcgacatagg
     1321 tgtagcggat gtaagtggtg ttctggccgt gctccatcca gaccgtctta tggagcagcg
     1381 cgctcccaag gcggaaagcg gtggtgggta tgccgtcccg tatgctgaac ccctcgatat
     1441 gagcgaagcc ttcgggcaag acctggccgt ccgggtactc gttcgtgccc aggtagaagg
     1501 tgcgcccgcc aatcgtggct tcttcgtcaa tcttggagag gagcaccgtg cgctccaccg
     1561 gcggcttgag ggcagccacc agcagcccgt ggtaccggcg ggtgttcgct cccgccacgg
     1621 tgctggacgc gtagcccccg atgccgttag ccaccagcca ctctttagcg agcgcccggt
     1681 ccaggtctgc gcaaatctct cttggtacga ttatcataaa tttgggattt cgaaatgcga
     1741 atttcgaatt ggaaaggtcg aactgcagga ttcagctccg agaggtcaaa gagccaacaa
     1801 atccgcattt cgaagtttca aattccaaat ccgtatagca cgcttagtgc ctcgggtagc
     1861 tcgcctatgg tggtgatggt ggcgtcgggc acgatgctgg ggtcgtccac gtcgtcgggg
     1921 cggccctcta tgcgcttcca gatgcctttc atgccgaccg cgtgcgcgcc cgcgatgtcg
     1981 tagcggcgga agtcgcccac gaaagcggtc tccccaggct ccaggccgat gcaatctaat
     2041 gcggctcgga agatgggcgg ggcgggcttt tcccaaccca gctcgcacga gtagacgcga
     2101 cattccagca ggtcgagcag gccgaagcgt tccaggtcgg gatcgtgtat ggtggagggc
     2161 caggccgtgt tggacaccag ccccagcttg aggccgctat cgcgcagccg ctccagcgtg
     2221 ggctgcacgt cgggtcgcac ataggtcatg tcgctgacgc ccttgtacca gggcaccatg
     2281 tggtccaccc cggcgcgttc gggtatgccg cgctcgctga acagttcatc cagcaggttg
     2341 agcatgttga agctcttctg ggtgcgggcc gcctgctccc agccggaccg gaagcgcgcg
     2401 ttgaacacct ccaggaactc atcgaggggc ggcagcttgt cggcgtgccc ccgggaggcg
     2461 atgtaggtgt gcaggtcgct gttgagctgc acgtccagcg cctcccagtc ctcccgccgg
     2521 tattccagca aagtgccccc catgtcgaaa agcacgccct tcagcatgtg actgactcct
     2581 tcgtggttag taccgagtac cgagtgccga gtgccgagtg ccgagcaaag agacgtaaac
     2641 gcaaagacgc aaaggacgca aaggacgcgc aaagtttagg attgggttgg gtgcggggca
     2701 ctcggtactc ggtactcggt actcggcact tgtcattcct cccgctcttg gatgaactcg
     2761 taaacggcct gctgcacttt ctcccggtcg gtgtctaccg tcatgatgtg gaagccgcgt
     2821 tgcagggcta ccaggcgctt atcgtgcgag gccaggcggg aatagatgtg gtgcgagttc
     2881 atatagggca ccacgtggtc gcgccgggcg tacatgacga gggcgggcac cgtcacctgg
     2941 ccgagcacgc cgcgcacctg gcttatgaag tctatcagcg aggcggtggc cgcgcttggg
     3001 gaccgctcgt aggagacatg cctgctgtgg gcgagggggt cctctatgtc gcggtagggc
     3061 tggttgaggt agggcatctg gccctggatg ccccggagca ggctgccgat ggggggcggc
     3121 acgtaaatag gcgcgcccat cgtgatgatg cccgcgaggt cctggcccct gtgcgcggcg
     3181 gtatagaggg taagcgcccc tcccagcgac agcccggcca ggtagacctt ctcgcagcgc
     3241 gcaagcatgg catccaaagc tccgttcacc gaggcgagcc aatcgtgcca tgtggtgttc
     3301 gccaggtcct ccggggctgt accgtgcccc gcgaggagcg cagcccccgc cgtgatgccc
     3361 ctgtcggcca ggaagcgccc cagcccgcgc atctcgtaag gggtggaagt gaagccgtgg
     3421 agcatgagac agcccaccgg cccggcctcg tagaggaacg gctccgcgcc cggcatgagg
     3481 gggtacggac ccggcttagg ggcaagcggg gcggcgatat gctcaagcgg cccggtgctc
     3541 tttgctgaaa cgttagcagg catgtccatc gtgatagtat agcgcattgc gcggttgtgg
     3601 gcatctgcta acggccctgg ggtagccctg catgatgccc tgtggctaaa gccacgggct
     3661 gacaaggtac aaagtcccct acggggactg aggactacga gggtagtctc cgtaggagac
     3721 ttcgtccctt gtaagcccgc caattcattg gcagggcgtc ccccacaggg cgtccataca
     3781 ggccgtcatg caagaccacc cctcaccccc gccaaaaatg cttgcaactg catcagcaca
     3841 aggccgtata cacaaccggg tataaattcc gttgcagttt cccctcgcct ggtttatcat
     3901 cctctatgcg gccgtcacac gagaaggttg caggctataa aggcaggtca gggcctctct
     3961 caagcagtga cacttgccgt ctaccacacc gatggagcgc acatgaccca cgaatggcac
     4021 cgggacgatt acaccatcag caccgacccc aagcgcctcg acctggaggt gatacacggc
     4081 ttcctcgcta acgactccta ctgggcgcag ggcaggagcg tcgagacgat acggcgctcc
     4141 atcgagcact cgctggcgtt cggcgtgtac cagggcgaga ggcaggtagg cttctgccgg
     4201 gtagttaccg attatgcgac cctagcgtgg ctggccgatg tattcatagt ggaggcttac
     4261 agggggcgcg gcctggcggt gtggctggtg gacacggtca tctcgcaccc cgaattacag
     4321 ggcctcaagc gctggctgct agccaccaag gacgcccacg gcctgtatgc gaagtacggc
     4381 ttcgggccgc tgactgaggt tgaaaggtgg atgacacacc tcggcggcgg acggtaaccg
     4441 ctaagtgagc cgatgccact tgagctacac ccgcacgacc cgcgctgggc tgcctacttt
     4501 gatgaagtga aggcctcatt atccggggtg ctaggccgct atgcccccga tattcagcac
     4561 gtgggcagca cctcgattgc cggtattgtt gcgaaaccgg tgattgacgt tgcagtcgct
     4621 atagagcagt acccgctacc ggacgaggtg ctcgctgccg tggaggcgct gggctacaca
     4681 tactggggcg actatggcat cccgcaccgc catctcttct ttcagcgcgg cggacctgtg
     4741 gggtacaaca ttcacataaa cgagcttgcc aatgagcaat tccagaggca cgtgctcttc
     4801 cgcgactacc tgcgcgccca ccccgacgcc gcacaggaat acgagcgcct caagctggag
     4861 ttggcggcac aacatgaaga cgtaaatacc tatgcgaaca gcaagagcga gtttgtgcgg
     4921 gacattttga agaaggctca tttgtggcgg gaaggccgct gaagcagacg ttataggaga
     4981 gataaagata atgaaaaagc ggttatcgtt tttacttgta gcccttctcc tccccaccct
     5041 cgcggcatgc gacccgtccg gcttacccac agcccccacc cctaccatcg ccgcccagcc
     5101 taccgccgtc gaggcgcgat ccaccaacac gccccgcccc cgccgcaccc ccacgcctg
//
LOCUS       DASRWZ010000818         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1300528, whole genome shotgun sequence.
ACCESSION   DASRWZ010000818 DASRWZ010000000
VERSION     DASRWZ010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300528"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1130)
                     /locus_tag="VFH60_12990"
     CDS             complement(120..1130)
                     /locus_tag="VFH60_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013879094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6314744.1"
                     /translation="MPDKNKSEVVVITGASAGVGRATVQAFARRAARIGLLARGEEGL
                     EGARRDAERLGGKALVLPTDVADPEQVEAAAEAVEREFGPIDVWVNNAMTSVFSPFME
                     MTAEEFRRVTEVTYLGYVYGTQSALKRMLPRDRGHIVQVGSALAYRGIPLQSAYCGAK
                     HAVQGFTESVRAELIHEKSRVKIAMVQMPALNTPQFGWVKSRLPRKAMPVPPIYQPEV
                     AAEAVYWAAHHDRRHLYVGLSTIIVIWGDKLAPGLGDLYLGKTGYDAQQYDGPEDPNR
                     PHNLWEPLPGDRGAHGDFDEQANDTSAQLWADTHRSWLALAGAGVAGVAGLVAAAMSR
                     RK"
     gene            complement(1667..>3840)
                     /locus_tag="VFH60_12995"
     CDS             complement(1667..>3840)
                     /locus_tag="VFH60_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314745.1"
                     /translation="ATGCTHLIAGTYRVWEAIDGAVSGSSCYTNSPQLTKTEPQFPGW
                     GGDVYISQLQYAVSLSTTAIVGTNDGNVWYGFNLGQGTPNSATWVDVTGENEQLPNRP
                     VLDVTTDPITPTIGYAALGGFNESIKGQPGHLFQVTCLAPMCGGAMTVPGGANAPAGA
                     NFTWVDKSGNLPDLPLDAVVVNPRFRQQVFVGTDAGLFYTNDIDAAEPVWMRFTNGIP
                     NVWIGDLTIDRGFTTLAVWTKNRGLYVWPLPAAPFIQPSPTPSPTGTPPTATPTTTAG
                     GGTCSQVAVTTQEGTIIPANDDTGAYCDDCSPLINFPFPVRLYDRTFSQGYATSNGAI
                     AFDMVSLAYGDDCLPNRNETYTIHAFTSDLCTNQCTAENTACEGCGIFTSTVGEAPNR
                     KFIVEWRAEYFGAPYQVNFEVVFTEGSDTVSVIYGEDTTESSGAFANAGVQKDQTTYY
                     SFACNEELLTNGLQVNYTFSECPAATATPIGGDTCPIQFADVPPSSSASSFYPYVRCL
                     ACRNIIGGYPCGGTNPQTGAAEPCNASRDAYYRPNNNITRGQIAKIVAQSAGYSDEPG
                     LQVYADVPPDQVFWLYIQQLSNDNIMGGYACGGTDPYTGQPEVSDSENRPFFRPNAPA
                     TRGQIAKIVANTAGVVYAGSNQSYQDVPPSSSPTSFYPYIEGLTSMGVMSGYACGGPG
                     EPCVGPNNRPYFRPGKLVTRAQAAKIVANTFFPNCQTPARP"
BASE COUNT          621 a         1195 c         1278 g          746 t
ORIGIN      
        1 cgggcggctg atccttcgcg gagtttaccc tgagcgtagc cgaagggctc aggatgacaa
       61 agggggagtt ggtcctgcca attgttagtt ccgtccctat ccatctgagc acgtgatggc
      121 tacttacgcc tcgacatcgc ggctgcaacc aggcccgcga cacccgccac gcccgccccg
      181 gcgagcgcca gccagctacg gtgggtgtcg gcccatagct gcgcgctggt gtcgttggcc
      241 tgctcgtcga agtcgccgtg cgccccacgg tcgccgggca gaggctccca caggttgtgc
      301 gggcggttgg ggtcttccgg gccgtcgtac tgctgcgcat cgtacccggt cttgcccagg
      361 tagaggtcgc ccaggccggg ggccagtttg tcgccccaga tcacgatgat ggtggacagg
      421 cccacgtaca ggtggcggcg gtcgtggtgc gcggcccagt agaccgcctc ggctgccact
      481 tcgggctggt agatgggcgg caccggcatg gccttgcgcg gcaggcggct cttgacccag
      541 ccgaactgcg gcgtgttgag ggcgggcatc tgcaccatcg cgatcttgac gcggcttttc
      601 tcgtggataa gctcggcccg caccgactcg gtgaagccct gtaccgcgtg cttggccccg
      661 cagtaggccg actgcaacgg tatgcccctg taggccagcg ccgagccgac ctgcacgatg
      721 tgcccccggt cccttggcag catccgcttg agggccgact gcgtgccata cacgtagccc
      781 aggtaggtca cctcggtcac ccgccggaac tcttccgccg tcatctccat gaagggcgag
      841 aagaccgagg tcatggcgtt gttcacccac acgtcgattg gcccgaactc ccgctcgacc
      901 gcctcagccg ccgcctccac ctgctccggg tcggccacgt cggtaggcag caccagcgcc
      961 ttgccgccca acctttcagc gtcccgtcgc gccccctcta gcccctcctc gccccgcgcc
     1021 agcagcccta tgcgcgcggc tctcctggca aacgcctgca cggtagcccg ccccaccccc
     1081 gcgctcgccc cggttatcac cactacctca gacttgtttt tatccggcat tgtaggttcc
     1141 tttctcgaac gatggacgct gggacagcga tggggtgggg gatgctttgc ctaccccgcg
     1201 ccgtcgagac gccctgccac taatgaagat taacaacaga atgaggtaat cagcaggcgc
     1261 aagcagcatc tgtaggggca ggcacggggg cctgccccta cacactatta ccttattcag
     1321 ttcttaatct tcataagtgg cgggctggca aggggcaaag tctcctttgg ggactaggga
     1381 acatcatgta gtctccgaag gagacttcgt ctctggtcag cccgtggctt cagccacagg
     1441 gcgaggttgg cggggcggct cgggcctcat ggtttgcctc ccgcccatcg tctaccaacg
     1501 ttcgctctgc gttgatgttg cacgagcact cagtctgcac attccaggcc atccaagaat
     1561 tgccggcacc acccacaaac agagaaggga cccccgtgtg gaggtccctt cccgttggcc
     1621 catgcggttg accgcgcggg cataggtgtg ttagtaactc tgccccttag ggcctggcgg
     1681 gtgtctggca gttggggaag aaggtgttag ccacgatctt cgctgcctgc gccctggtga
     1741 ccagcttgcc gggacggaag taaggccggt tgttcgggcc tacacaaggc tcgcccggac
     1801 caccgcacgc gtagccgctc atcacgccca tgctcgtcag cccttcgatg tacgggtaga
     1861 acgaggtcgg gctgctgctc ggcggcacgt cctggtagct ctggttggag ccggcgtaca
     1921 cgaccccggc cgtgttagcc acgatcttgg ctatctggcc gcgggtggcg ggagcgttcg
     1981 ggcggaagaa cggcctgttc tccgagtcgc tcacttccgg ctggcccgtg taggggtcgg
     2041 tgccgccgca cgcgtaacca cccatgatgt tgtcgttgga caactgctgg atgtagagcc
     2101 agaacacctg gtcgggcggt acgtcggcgt agacctggag acctggctcg tcggagtaac
     2161 cggcggactg ggccacgatc ttggctatct ggccgcgtgt gatgttgttg ttgggcctgt
     2221 agtaagcgtc gcggctggcg ttgcaaggct ccgccgcgcc cgtctgcggg ttggtgccgc
     2281 cgcacgggta gccgccgatg atgttgcggc aggcgaggca gcgcacgtat gggtagaacg
     2341 agcttgcgga gctgctagga ggcacgtccg cgaactggat cgggcacgtg tcaccgccga
     2401 taggggtggc agtagccgcg gggcactcgc tgaacgtgta gttcacctgt agcccgttcg
     2461 tgagcagttc ttcgttgcag gcgaagctgt agtaggtagt ctggtccttc tgcacgcccg
     2521 cgttggcgaa cgcgccgctg ctctcggtag tgtcctcgcc gtagatgacg gacaccgtgt
     2581 ccgagccttc ggtgaagacc acctcgaagt tcacctggta cggtgcgccg aagtactccg
     2641 cgcgccactc aacgatgaac ttgcggttcg gggcctcgcc caccgtggag gtgaagatac
     2701 cgcagccttc gcaggcggtg ttttccgcgg tgcactggtt ggtgcacagg tcggaggtaa
     2761 aggcgtggat ggtgtaggtc tcgttcctgt tcggcaggca gtcgtcgcca taagcgaggc
     2821 tgaccatgtc gaaggctatc gcgccgttgg aggtcgcgta accctggctg aaggtcctat
     2881 cgtacagcct gacggggaac gggaagttaa tcagaggcga gcagtcgtcg cagtaagccc
     2941 cggtatcgtc gttggccggt attatcgtgc cttcctgggt cgttaccgct acctgggagc
     3001 aggtgccccc gccggcagtg gtggtaggcg tagcggtggg cggcgtgccc gtggggctag
     3061 gcgtagggct gggctggatg aacggagccg caggcagcgg ccacacgtac aggccgcggt
     3121 tcttggtcca cactgccagc gtggtgaagc cacggtctat cgtcaggtcg ccaatccaga
     3181 cgttgggtat gccgttggtg aagcgcatcc atacaggctc ggccgcgtcg atgtcgttgg
     3241 tgtagaacag acccgcgtcg gtaccgacga acacctgctg gcggaaccgc gggttcacga
     3301 ccacggcatc caggggcagg tcgggcaggt tgccggactt gtcgacccag gtgaagttgg
     3361 ccccggcagg cgcgtttgcc ccgcctggaa ccgtcatggc cccgccgcac atcggagcaa
     3421 gacatgtcac ctggaacagg tggcccggct ggcccttaat cgactcgttg aagccaccca
     3481 gggcggcata gccgatagtc ggggtaattg ggtcggtagt cacgtcgagc acggggcggt
     3541 tgggcagttg ttcgttttcg ccggtaacgt ctacccacgt agccgaattg ggggtaccct
     3601 ggcccaggtt gaagccgtac cagacgttgc cgtcgttggt gcccacgata gcagtggtgg
     3661 acaggctcac ggcgtactgg agctggctga tatagacgtc gccgccccaa ccggggaact
     3721 gaggctcggt cttggtcaac tgcgggctgt tggtgtaaca actgctgccc gaaaccgcgc
     3781 cgtcaattgc ttcccacacg cggtaggtgc ccgcgatgag gtgggtacaa ccggtggccg
//
LOCUS       DASRWZ010000819         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1025502, whole genome shotgun sequence.
ACCESSION   DASRWZ010000819 DASRWZ010000000
VERSION     DASRWZ010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025502"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /locus_tag="VFH60_13000"
     CDS             complement(<1..1983)
                     /locus_tag="VFH60_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET6314746.1"
                     /translation="MRRVVLVTDGWETRGRAADEASRLSARGIDVQVVALPALGEPEV
                     VVQYVSTTPYVRVGDYLQSDVSVFSTVETSATLRVSIDGGEPSVREVLLRRGQNQIPL
                     EQRAEVSGFHRIDVQVDTPRDTASENNTASVTLVVKGEPRVLVLEDRPGEGQSIADAL
                     RSQQMTVDVRAPATIPAQVGELADYDAIIMNNVAATSMTLDQQKTIQEYVRRNGRGLV
                     VVGGETSYSRGGYLDSVFEEMLPVSSDPAPRPEQGATALMLVLDRSGSMSLHEGTGPA
                     SLIQKSDMAKEAARLAVDALREGDSLGVLAFDDINWWIVPVQTIRGQADKDRAKALIS
                     ELEMGGGTNIYPAVKEGAEALRSVLTRNKHMVLLTDGQEERPPDYGPLMEQIRAEGVS
                     LSAIAIGQSADTDLLTRLAKLGQGRYYFTEQPANIPKIVFKELNLALKDATIVGRVQP
                     HFQAPSPVLRGFAPQDLRQLSGYNITTAKDDAVMALVSDQNDPLMAHWNYGLGRVAAF
                     TSDANSLWAADWLLWEDFGRFWSGVVRWTMASPVSRQLQPAITVVQQGDQDVAVLSVE
                     SLNADNSFANLTDLTAALRSPSGAITNVAMSQAAPGRYEARIPLAEKGAYEVRLSRGG
                     DQPAIETAGFSVPPSAEFLHAGTNDRLLRQLNGGKAF"
BASE COUNT          323 a          677 c          666 g          446 t
ORIGIN      
        1 aaaggcttta ccgccgttga gttgcctgag gaggcggtca ttggtgcccg cgtggaggaa
       61 ttcggcgctg ggaggcacgc tgaagcccgc cgtctcgatg gcaggctggt ccccgccccg
      121 cgagagcctg acctcatatg cgcccttctc ggccaggggt attcgtgctt cgtaacgccc
      181 cggcgcggcc tgcgacatag caacgttcgt gattgcacct gaagggctgc ggagggcggc
      241 tgtgaggtcg gtgaggttgg cgaagctgtt gtccgcgttg aggctttcca ccgacagcac
      301 ggctacgtcc tggtcgccct gttgcaccac cgtgatggcg ggctgcaatt gcctgctcac
      361 cggacttgcc atcgtccacc gcacgacgcc gctccagaag cgcccgaagt cctcccacaa
      421 cagccagtcg gcggcccaga gggagttggc gtcggaggtg aaggcggcta ccctaccgag
      481 gccgtagttc cagtgcgcca tcaaggggtc gttctggtcg gatacaaggg ccataaccgc
      541 gtcgtccttc gcggtggtga tgttgtagcc gctcaactgg cgcaggtcct ggggcgcgaa
      601 tccacgcagt acggggctgg gggcctggaa atggggctgc accctgccca cgatggtggc
      661 atctttgagc gccaagttca actctttgaa gactatcttg gggatgttag cgggctgctc
      721 ggtgaagtag tagcgcccct ggcccagctt ggcgagcctg gtcagcaggt ctgtgtccgc
      781 gctctggcct atggctatgg ccgacaggct cactccctcg gccctgatct gttccatcag
      841 tggaccgtag tcgggtgggc gctcttcctg cccgtcggtc agcaagacca tgtgcttgtt
      901 gcgcgtcagc acggagcgca acgcctccgc gccctctttc accgccgggt agatgttggt
      961 gcctccgccc atttcaagtt cggagatgag ggccttcgcg cggtccttgt ctgcctggcc
     1021 ccggatggtc tgcacgggca ctatccacca gttgatgtcg tcgaaggcca gcacgcccag
     1081 cgaatcacct tcgcgcaggg cgtccaccgc cagccgcgcc gcctctttcg ccatgtccga
     1141 cttctggatg aggctggcag ggccggtccc ttcgtgcagg ctcatgctgc cggagcggtc
     1201 cagcacgagc atgagggctg tcgcgccctg ctcaggtcgc ggggcggggt cggacgacac
     1261 gggcagcatc tcctcgaata cgctgtcgag gtagccgccc ctggaatagc tggtctcgcc
     1321 gcccacgacg acgaggccgc gcccgttgcg gcgcacgtac tcctggatcg tcttctgctg
     1381 gtcgagggtc atgctggtgg ctgccacgtt gttcattatt atggcgtcgt agtcagctaa
     1441 ctcgcccacc tgcgccggga tcgtggcagg ggcgcgcacg tccacagtca tctgctggct
     1501 tctcagcgca tcagctatgc tctgcccttc ccccggcctg tcttccagca ctaacactct
     1561 cggctcaccc ttgaccacca acgtgacgga agcggtattg ttctccgagg cggtgtcgcg
     1621 gggggtgtct acctgcacgt ctatcctgtg gaagcctgat acttcggctc gctgctcaag
     1681 cggtatctgg ttctggcccc ggcgcagcag gacctctctg accgatggct ccccgccatc
     1741 tatgctcacc cgcaaagtgg cgctggtctc gacggtgctg aatacgctca cgtcgctctg
     1801 caagtaatca ccaacgcgca cgtatggggt ggtcgaaacg tactggacca ccacttccgg
     1861 ctctccgagg gcgggtaggg cgactacctg cacgtctatg ccgcgtgcgg agaggcgaga
     1921 ggcttcatcg gcagcccgcc cgcgcgtctc ccagccgtcc gtgacgagca cgacccgccg
     1981 catgcccgtt tcgttcagga ggctgcctgc aaggcgtaac cctgccgcaa aatcacttcc
     2041 attttggatt ttcgattttg gattttcgat tggattttcg gtttgtccga ggtttgcttc
     2101 taaccaggag tt
//
LOCUS       DASRWZ010000820         6401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1368072, whole genome shotgun sequence.
ACCESSION   DASRWZ010000820 DASRWZ010000000
VERSION     DASRWZ010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368072"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /gene="pyrR"
                     /locus_tag="VFH60_13005"
     CDS             complement(<1..539)
                     /gene="pyrR"
                     /locus_tag="VFH60_13005"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET6314747.1"
                     /translation="MPKPEPIVLLDGEAIRRALTRIAHEILERNSGVGDLALVGIKTR
                     GETLATRLSALIEKFEGTAVPVAELDVTPYRDDRPELRGQGARGEVPGLGMEVERRTI
                     VIVDDVFYTGRTVRAAIDGIMDNGRPASIQLAVLVDRGHRELPIRADYVGKNVPTSRA
                     ERVQVHITETDGLDEVVLLR"
     gene            1111..1539
                     /locus_tag="VFH60_13010"
     CDS             1111..1539
                     /locus_tag="VFH60_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6314748.1"
                     /translation="MNTPKHKPTDTTAGAGTATLVQIVTGEDTCEVRCVHPGNVRRGR
                     DLLLPPGEYQALADTFQALSDPSRAKIVYSLLEQELCTCDLAAIVGVSESAVSQHLNV
                     LRRLRLVRSRREGKIIYHTLDDAHIKTLLAVCLEHVRDEE"
     gene            1753..4158
                     /locus_tag="VFH60_13015"
     CDS             1753..4158
                     /locus_tag="VFH60_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET6314749.1"
                     /translation="MITTKKNHTSDTTEKLRISGMDCGDCAVSIEKSLETLPGVRSAS
                     VSFGAGTAQVTYDPALVGHAAIARRVSELGYNVEETGAASMQTGPLEFIIEGMDCGDC
                     ARTIEKSVAALPGVAQASVNFGSARLSVVPSADSERPQASTIERVVTAAGYRATPVEK
                     RRTDASMPFWKRERRVISTVIGTVLAMLGFALSFTPAPVWLVNAFFGAGILIAGLAFA
                     RAGLLALRAGRADMNLLMTVAAIGAVLLGDWGEAVTVVLLFAFGGTLQSYTLDKTRGA
                     IRALMDLTPATALVRRADTSTGLPIMREVRLPVEEVRPGETVLVGPGERLPLDGVVLQ
                     GASSIDQSSITGESVPVDVEPGAEVFAGTINGPGALGIRTERAAKDTTLARIIEMVEQ
                     AQARRAPSQQFVDRFSAVYTPVVIAAATLVAVLPTLFFAQPFNDWFYRALVLLVVACP
                     CALVISTPVSIVSAIGAATRRGVLVKGGATLEALGKVRAVAFDKTGTLTEGRPRVVAV
                     RALDADTDGLLRLAATVEARSEHPLAMAIVHAGRNLKHGAPEAAQFTAFPGLGAKAWV
                     DGREVYVGNARLFREQGIPTDGVDSLLEDEQEQGRTVVIVGTREGALGVVSLADSLRH
                     DARQAVQGLREAGIGYVAMLTGDNERTARAIAREVGVDECYAGLLPADKLAAVERITK
                     ERGPVAMVGDGVNDAPALAAAEVGIAMGVSGTDAALEVADVALMSDDLSRLNYALQLS
                     RRTLSTIQANVAASLIVKALALALAALGMLPLWGAIFADMGVSLLVTLNGMRLLGYGR
                     RKA"
     gene            complement(4476..5381)
                     /locus_tag="VFH60_13020"
     CDS             complement(4476..5381)
                     /locus_tag="VFH60_13020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6314750.1"
                     /translation="MKLGLQVNHFNWPGGEASIGQTFRDIGTRAEQAGFDSLWVMDHF
                     FQIPGLGPAEYAMLEGYTALGFIAGATSKIKLGTMVTGVTYRHPGILVKTATTLDVLS
                     GGRAYFGVGAAWFEREHQALGVPFPPLKERFERLEEMLQIAHQMWSGDVGPYNGKHYQ
                     LAETLNVPQSISKPHPPILIGGTGEQKTLRFIAKYGDACNIFLRLGDDVVRHKLDVLR
                     GHCEAEGRPYEEIEKTSLERLHVTRDGSGDTMSPQQAIDHFAETAALGFDHAIFSMPN
                     VYEPDAFEVFHEIAPAIHDIPVAGR"
     gene            5750..5944
                     /locus_tag="VFH60_13025"
     CDS             5750..5944
                     /locus_tag="VFH60_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314751.1"
                     /translation="MLSELYEADLAANGFIPNYARAFSLRPEVYQAWRTLIGSIRGNM
                     DLRRYELVTIAAAAKLRCSY"
     gene            5945..6298
                     /locus_tag="VFH60_13030"
     CDS             5945..6298
                     /locus_tag="VFH60_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314752.1"
                     /translation="MLAHGAVLRSKFFSPEEVEAIARDYRSAGLSEVDVAVMALAEKV
                     TLNAYKVTEEDLEKLRAHGLADSDILDIILAASARCFFSKVLDATGAEPDEEAYGTMA
                     PELRVALVVGRPFGE"
BASE COUNT         1153 a         1944 c         2123 g         1181 t
ORIGIN      
        1 cggaggagca ccacctcgtc aagaccgtct gtctcggtga tgtggacttg cacgcgctcg
       61 gcgcgggagg tggggacgtt cttgccaacg tagtcggcgc gaatgggcag ctcgcggtgc
      121 ccacggtcta ccagcacggc cagttgtatg ctggcggggc ggccgttgtc catgatgcca
      181 tcgatggcgg cgcgcaccgt gcgcccggtg tagaacacgt cgtccacgat cacgatggtg
      241 cggcgctcca cctccatacc gaggcccggc acctctcccc tcgcgccctg gcccctaagc
      301 tccggcctgt cgtcacggta gggggtgacg tcaagctcag ctaccgggac ggcggtgccc
      361 tcgaacttct cgatgagggc cgaaaggcgc gtggccagcg tctcgccgcg cgttttgatg
      421 ccgaccaggg ccaggtctcc cacgccgctg ttgcgctcca gtatctcgtg cgctatgcgt
      481 gtgagggcgc gacgtatcgc ctcgccgtcg agcagcacaa taggttcggg tttgggcatg
      541 atatctactg cttcttccta cggcgtatgt tgaacgcggg cactccaccc gcggccagcc
      601 acaacacccc caacatcacg agcaaaatcc aatccacacg catggcaaat acccccggtc
      661 tcaagaccac cgccggccag aaccggcgca tcaatttcgc ggatgctaca cccagcgggg
      721 acgtacatcc gacgttttgc acgtctgtgg atttcggaat tgttgaggtg gtcagcatca
      781 tgttcgctgg tgatggaaga tcacaactgc attgatattg gatagcagga ccaaagtggc
      841 ctttgtcatt ctgagagtag cgaagaatct aaggaagctg gcaatgggag gctgttgaac
      901 gttgcatccg atgcggctaa tgctccacgt accggcaggg gcggcatctt agattcttcg
      961 ctacgctcag aatgacaaag gccactttgg tctctctact tgcaatagga ccaatttggc
     1021 aaatgacgga accaagactg aatagctatt gccttcccgt gtaaagagtg ttaccattag
     1081 taacgattta gcgtatactt aattgagact atgaacacac ccaaacacaa accaacagac
     1141 acaacagcag gcgcaggcac cgcgaccctg gtgcaaatcg tgacgggcga ggacacctgc
     1201 gaagtacgct gtgtgcaccc cggcaacgtg cgccggggcc gcgacctcct gctgccaccg
     1261 ggcgagtacc aggccctggc agacaccttc caggcgctct cggaccccag ccgcgccaaa
     1321 atcgtctaca gcctgctgga acaggagctt tgcacctgcg acctcgcggc catagtgggc
     1381 gtcagcgagt cggcggtgtc ccagcacctg aacgtgctgc gccgcctgcg cctggtaagg
     1441 agccgccggg agggcaaaat catctatcac accctggacg atgcccacat caagacgctg
     1501 ctggccgtct gtctggagca tgtgagggac gaggagtaac gagtgccgag tagccctgtg
     1561 gccgtcgccc agccaatgaa tgggcgggct tacacggtac aaagtcccct acggggacta
     1621 agcaacattg ggtagtctcc gagggagact tcgtctctgg taagcccgcc aattcattgg
     1681 cagggcgtcc cggcagggcg aaccagacag gacatcacgc ggggcgtcct aagcgcctct
     1741 gatcagacaa ccatgattac gactaagaaa aaccacacat ccgacacaac cgagaagctg
     1801 cgcatcagcg gcatggactg cggcgattgc gccgtgagca tcgagaagtc gttggagacg
     1861 ctgccggggg tgcgctcggc cagcgtgagc tttggggcgg gcacggcgca ggtgacgtac
     1921 gacccggcgc ttgtcggaca cgcagccata gccaggcgtg tatcggagct tggctacaac
     1981 gtcgaagaga ccggggcggc gagcatgcag accgggccgc tcgaattcat catagagggc
     2041 atggactgcg gcgattgcgc gcgcaccatt gaaaagtccg tggcggcgct gcccggtgtg
     2101 gcccaggcaa gtgtgaactt cggctcggcg cggctgtcgg tggtgccctc agcggactcg
     2161 gagcgtcccc aggcgagcac aattgagcgg gtggtgacgg cggcgggcta cagggccaca
     2221 cccgtggaga aacgccggac ggacgcaagc atgccgttct ggaagcgcga gcgccgggtt
     2281 atcagcactg tcataggcac cgtcttggct atgctgggct tcgcgctgtc atttacgccc
     2341 gccccggtct ggctggtcaa cgccttcttc ggtgccggca tcctcatcgc ggggctggct
     2401 ttcgccaggg cggggctgct tgcgctcagg gccggacgcg ccgatatgaa cctgcttatg
     2461 acagtggcgg ccataggcgc ggtgctgctg ggcgactggg gcgaggcggt cacggtggtg
     2521 ctgctcttcg cgttcggcgg caccttgcag tcttacacgc tggacaagac gcgcggtgcg
     2581 attcgcgccc tgatggacct cacgcccgct accgccctgg tgcgccgcgc cgacacgagc
     2641 accggcctgc ctatcatgcg cgaggtccgc ctgcccgtcg aggaggtgcg gcccggcgag
     2701 accgtgctgg tcggccccgg cgaacgcctg cccctggatg gcgtcgtgct gcaaggcgca
     2761 tcgtccatcg accagtcgtc catcacgggc gagagcgtgc cggtggacgt ggagcctggg
     2821 gcggaggtgt tcgcgggcac catcaatggc cccggcgcgc tcggcatacg cacggagcgg
     2881 gcggccaagg acaccaccct ggcccggatc atagaaatgg tcgagcaggc gcaggcgagg
     2941 cgcgccccgt ctcagcagtt cgtggaccgc ttctcggctg tgtacacgcc ggtggtgatt
     3001 gcggccgcca cgctggtcgc cgtgctgccc acattgttct tcgcgcagcc gttcaacgac
     3061 tggttctacc gcgccctggt gctgctcgtg gtggcctgcc cctgcgccct cgtgatctcc
     3121 acccccgtga gcatcgtgtc cgcgattggc gctgccaccc ggcggggcgt gctcgtcaag
     3181 ggcggggcta cgttggaggc gctgggcaag gtcagggcgg tggcgttcga caagaccggc
     3241 accctcaccg aagggcggcc ccgcgtggta gccgtgcgcg ccctcgacgc tgacaccgac
     3301 ggcctgctca ggttagccgc cactgtggaa gcccgctccg agcaccccct cgcgatggct
     3361 atcgtgcacg cgggccgcaa cctcaagcac ggcgcgcccg aagccgctca gtttaccgcc
     3421 ttccccggcc tgggcgcgaa agcctgggtc gatgggcgcg aggtgtacgt gggcaacgcc
     3481 cgcctcttcc gcgagcaagg catccctacg gacggtgtgg actcgctgtt ggaggacgag
     3541 caggagcagg gccgcaccgt ggtaatcgtg ggcacgcgcg agggagcgct gggggtagtc
     3601 tcactggccg acagcctccg gcacgacgcg aggcaggcgg tgcagggcct gcgggaggcg
     3661 ggcatcggct acgtggcgat gctcaccggg gacaacgagc gcaccgctcg tgcgattgcc
     3721 cgcgaagtag gcgtggacga gtgctacgcc ggcctgctgc ccgccgataa gctggccgcg
     3781 gtggagcgca taacgaagga gcgcggcccc gtggcgatgg tgggcgacgg cgtaaacgac
     3841 gcgcccgcgc ttgccgccgc cgaggtgggt atagcgatgg gcgtgtccgg caccgacgca
     3901 gccctggaag tggccgacgt ggccctgatg agcgacgacc tctcgcggct caactacgcc
     3961 ctgcagctgt cgcgccgcac actctccacc atccaggcga acgtggcggc ctccctcatc
     4021 gtgaaggcgt tagccctggc gctggccgcg ttgggcatgc ttccactgtg gggcgcgatc
     4081 ttcgcggata tgggcgtgtc gctcctggtc acgctcaacg gcatgcggct gctgggctac
     4141 ggcaggcgca aggcttagtg ttccgcgcgc cggactagcc ggttattctt cattcgccac
     4201 agggcgttgg cctttgcctg ttgttgccag ctagtaccaa atctctttga gcagactaga
     4261 aaaaaagaag ggaaaataag aatgggggac acccccagcc ccccgtcagg ggagtacccc
     4321 tgcacccccg tgtgagggag agcactgggg gacactccca gccccacgtc aggggagtat
     4381 ccctgcaccc ccttgtgagg gagagcactg ggggacaccc ccgccccagt caggagaccc
     4441 ccctgcaccg cctgcgtgac cctatcgcgt gcgggttatc taccggctac cgggatgtcg
     4501 tggatggcgg gggcaatttc gtggaagacc tcgaaggcgt caggctcata cacgttgggc
     4561 atgctgaaga tggcgtggtc gaagcctaag gcggccgttt cggcgaagtg gtctatagcc
     4621 tgctggggcg acatggtgtc tccactgccg tcgcgggtga cgtgcaggcg ttccaggctg
     4681 gtcttctcga tttcctcgta ggggcgacct tccgcctcgc agtggcctcg cagcacgtcg
     4741 agcttgtggc ggaccacgtc gtcacccagg cgcaggaaga tgttgcaggc gtcgccgtat
     4801 ttggcgatga agcgcagcgt cttttgctcg ccggtgcctc cgatgaggat gggcggatgg
     4861 ggcttgctga tggactgggg cacgttgagg gtctcggcta gctggtagtg cttgccgttg
     4921 taggggccga cgtcgccgga ccacatctga tgggctattt gtagcatctc ttccaggcgc
     4981 tcgaagcgtt ccttgagggg cgggaacggc acgccgagag cctggtgctc gcgctcgaac
     5041 caggccgcgc cgacgccgaa gtaggcacga ccaccggaga gcacgtcgag ggtggtggcg
     5101 gtcttgacga ggatgccggg gtggcggtag gtgacgccgg tgaccatagt gcctagcttg
     5161 attttggagg tcgctcccgc gatgaagccg agggcggtgt agccttcgag catggcgtac
     5221 tcggcagggc cgaggccggg gatctggaag aagtggtcca tgacccagag gctgtcgaag
     5281 cctgcttgtt cggcgcgggt gcctatatcg cggaatgtct gtcctatgct tgcctctcca
     5341 ccgggccagt taaagtggtt tacctgcaat ccgagtttca ttggtatgta tctcctcgtg
     5401 tgtgttacgt tgttttttgc cgttcgtacg tgtatagcct catgttcaca agaagtgtgc
     5461 cgcctctgtg ttagggtggg gtgtattcgt ttgtaaagga gcgctatttg gctgcggagg
     5521 tagaatgtgg gcgcttaggg tgcagtataa cacaagggag gcggtagcct gggcgagttc
     5581 tgcaagcatc accactcccg ccattcccct ttacaggtcg ttcggcttcg gatataagtt
     5641 aggcatctcg aagctgtttt gcagcccggc aagggagggc ctgcgaaacc cacataggag
     5701 gtagagggtg tcgtacatac agactgtgcc acatgaagaa gcatcaggct tgctcagcga
     5761 actatacgag gcagacctgg ccgccaacgg cttcatcccg aactacgccc gcgctttcag
     5821 cctgcgcccc gaggtatacc aggcctggcg caccctgata ggctccatca gggggaacat
     5881 ggacctccgg cgctacgagc tggtcaccat cgcggctgcg gccaaactca ggtgcagcta
     5941 ttgaatgctg gctcacgggg cggtgctccg ttcgaagttc ttcagccctg aggaggtcga
     6001 ggctatcgcc cgcgactacc ggagcgccgg tttgagcgag gtggatgtag cagtgatggc
     6061 cctcgcggag aaggtgaccc tcaacgccta caaagtgacg gaggaggact tggagaagct
     6121 gcgcgcccac ggcctggccg actcggacat cctcgatatt attttggcgg cctccgcgcg
     6181 ttgcttcttc agcaaggtgc ttgatgctac cggcgcggag cctgacgagg aagcgtacgg
     6241 cactatggca ccggagctgc gcgtggcact ggtggtaggg cggcccttcg gcgagtaata
     6301 ccaccgacct gctttagccc tgtggctaat gaagatcaag aagttatcca acacacatca
     6361 ccagccgacg cccatcgatt tatgaagatt atgaacttga t
//
LOCUS       DASRWZ010000821         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_410191, whole genome shotgun sequence.
ACCESSION   DASRWZ010000821 DASRWZ010000000
VERSION     DASRWZ010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410191"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            550..1965
                     /locus_tag="VFH60_13035"
     CDS             550..1965
                     /locus_tag="VFH60_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET6314753.1"
                     /translation="MKNNSKTRAAINKRRYSSRLAMVALALVLAAFVAASGTWSTASA
                     QSPQPKPERTSPQAACPPDGQCFADVLPSNPFYNYANTLYREDIVTGYACGGAGEPCD
                     ASRRPYFRPNSAVTRQQMTKFIDAARTQPGIFINTSTSALPLYSRTTVLDGIALYGES
                     TNGDAISALASASNRSAVLARNTGGGFGVYADVTGNGYGVWARSGGQALHGETTGNAD
                     AVVGRNGTSGRSGVYGLHTGSGLGVTGRSTSGIGVLGDSAGDNGVMGQSSSSSDSGVY
                     GYNTNGGFGVAGRTNANSGIFAAVFGENTGGGYAAYFSGATNTRGTGTASLGTYTIDH
                     PQDPANKYLNMSAVAGPEAMNVYNGNVTTDSNGDATVTLPAYVESLNKDFRYQLTVIG
                     TFAQAIVSTEIQKNSFTIKTDKPNVKVSWQVTGVRKDPYAEKSQPPVEQDKPSLERGY
                     YRHPEVYGQPDSKSVQDAYQP"
BASE COUNT          454 a          703 c          661 g          386 t
ORIGIN      
        1 aggaatcagg tgggtaggga agaaggcgaa ttgcagtacc gagtaccaag tacggagtac
       61 cggagtatgc ttgctggcgt acccatatcg gggtgcctcg ccactcgcct gctgtcctcc
      121 gactgagtac gactgccacc tgattcctcg ctacgctcgg aatgacaagg gccactttgc
      181 gcctgctatt agattcttac ttgcaagatg caccgctcgg aacgacatcg gtggggcttc
      241 gggtactcca atctcccacg cgagttccta cgatgtcttc tagcttcttc cagcaagagt
      301 tatctttgtt ccagggtgcg accaccgcgt catttgcccg tttaaggatg gcgtcgggcg
      361 ataaagggac ggcggcagtc cctggatagg catgttcgtc gcctggctgt gctgcgggga
      421 atctgcccgg catattggat gcgaatttgg gggtgcgcgc catgaaagtg tcatagaatg
      481 gtggcgagcc aatcaggttt gctttgtttg catttacatc gtgggagcag gaggaaagga
      541 gacactacaa tgaaaaacaa cagtaagacg cgggccgcca taaacaagcg gcggtactct
      601 tcacgtctgg cgatggtagc gctcgccctg gtgcttgcgg cgttcgtggc ggcgagcggc
      661 acctggagca cggcctcggc ccagtcgccc cagcctaagc ctgagcgcac gtcgccccag
      721 gcagcctgcc cgcccgacgg ccagtgcttc gcggacgtgc tgccgtccaa ccccttctac
      781 aattacgcta acaccctgta ccgggaggac attgttaccg gctacgcgtg cggcggcgca
      841 ggcgagccgt gcgatgcgtc caggcggcca tacttccgcc ctaacagcgc cgttacgcgc
      901 cagcagatga ccaagttcat tgacgctgcc cgcacccagc ctggtatctt catcaacacg
      961 tctacctcgg ccctgccgct ctactcgcgc acaaccgtgc tcgacggcat cgcgctgtac
     1021 ggagaaagca ccaacggcga tgccatatcg gccctcgcgt cggcatctaa ccgcagcgcc
     1081 gtgctcgcac gcaacaccgg gggcggcttc ggcgtctatg cggacgtcac gggtaacggc
     1141 tacggcgtgt gggcgcggtc cggcggccag gcactgcacg gcgagaccac cggcaacgcc
     1201 gacgccgtcg tcggccgcaa cggcacctcg gggcgtagtg gcgtctacgg cctgcacacg
     1261 ggcagcggcc tcggcgtcac cgggcgcagt acgagcggta tcggcgtgct tggcgacagc
     1321 gctggagaca acggcgtgat gggccagtcc agctccagtt cggacagcgg cgtgtacggc
     1381 tacaatacga acggcggctt tggcgtggcc gggcgcacca acgccaattc aggcatcttc
     1441 gcggcggtgt tcggcgagaa cacgggcggt ggctacgcgg cctacttctc cggggccacc
     1501 aatacgcggg gcaccggcac cgcctcgctg gggacctaca ctatcgacca cccgcaggac
     1561 ccggccaaca agtacctcaa catgtcagcc gtggcaggcc cggaagcgat gaacgtctac
     1621 aacggcaatg tcaccaccga ctccaacggc gatgccacgg taacgctgcc cgcctatgtg
     1681 gagtcgctga acaaggactt ccgctaccaa ctgaccgtga taggcacttt cgcccaggct
     1741 atagtgtcca ccgaaataca gaagaacagc ttcacgatca agaccgacaa gccgaacgtg
     1801 aaggtctcct ggcaggtgac aggcgtgcgc aaggacccct acgcagagaa gagccagccc
     1861 cctgtcgagc aggacaagcc cagcctagag cgcggctatt atcgccaccc cgaagtgtac
     1921 ggccagcccg acagcaagag cgtacaggac gcataccagc cctaaaagag acggaaccgc
     1981 gcaggacgct aaggtaaata ggctgttctg agcacaaaag gaccagggta cagatgacgt
     2041 accctggtcc ttttacatgt gccacaatcc tttacctcgg cggttccctc tcctagccgc
     2101 cagggtgcca cctttgccgc tgtgacgcaa ctggcgaagc accgctatga ggactctctc
     2161 ccacgatggt agtctccgaa ggagacttcg ctacttccca gccc
//
LOCUS       DASRWZ010000822         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_340590, whole genome shotgun sequence.
ACCESSION   DASRWZ010000822 DASRWZ010000000
VERSION     DASRWZ010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340590"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..>1531)
                     /locus_tag="VFH60_13040"
     CDS             complement(73..>1531)
                     /locus_tag="VFH60_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6314754.1"
                     /translation="TFGRGRSLPGRVLAHGKPAWVSNVTNDPYFIRAPFARKAGLRAA
                     FASPITLGTEMVGVLEFFSKQVLEPDEPLLEMMTNIGAMLGRVVERSRARQALMESEA
                     KYRLLVDQASDGIAVYDQRGTILEVNSRASEMMGLPRDGLLGLNVTQVIAPEDLEKTP
                     LRFEELKGGEPVIGERVLVRPNGSRLPVELSARMLSNGNVQVIVRDITLRKLAEEQIQ
                     RLNEDLERRVAERTAELQEANRRLEEARQRAEEAVHIREELLSVVSHDLKNPLAAILG
                     NTQMLKRRVDRSANDDKEQWLRTVDRISRAANKMHNLVDELLDFGRLQAGQPLTLQFT
                     STDLVELAGQVVEEHQQSTNMHRVVLEAEVPSLIVRSDANRLERVLGNLLSNAVKYSP
                     GGGEIRVRVGREVDAEQEWAVLSVRDEGLGIPEHDLPHIFEWFRRAQNYSGRISGAGI
                     GLASANQIVKQHNGTIEVSSTEGQGSTFTVRLPIQ"
BASE COUNT          234 a          502 c          481 g          314 t
ORIGIN      
        1 accgttgcag gacgtgcatg cagggcaccc cgccagcctc actaccgaga atgcctgcac
       61 cgggcattgc cgctattgta tcgggaggcg cacggtgaag gtagagccct ggccttcggt
      121 gctgcttacc tctatcgtgc cgttgtgctg cttgacgatc tggttggcgc tggcgaggcc
      181 gatgcccgca ccgcttatcc tgccggagta gttttgcgcc cggcggaacc actcgaatat
      241 gtggggcagg tcgtgttcgg ggatgcccag gccctcgtct cgcaccgata gcaccgccca
      301 ttcttgctcg gcatcgacct ctctgccgac gcgcacccgt atctcgcccc cgccggggct
      361 gtacttgacc gcgttggaga gcaggttgcc aagcacgcgc tccagcctgt tggcatccga
      421 cctgacgatg agcgagggca cttcggcctc caggaccaca cggtgcatat tggtgctctg
      481 ctggtgctcc tcgactacct gcccggcaag ctccaccagg tcggtggaag tgaattgcag
      541 agtgaggggc tgcccggctt gcaagcgccc gaagtctagc agctcgtcca cgaggttatg
      601 catcttgttg gcggcgcggc tgatgcggtc cactgttctg agccactgct ctttgtcgtc
      661 gttggcggac ctgtctaccc ggcgcttcag catttgcgtg ttgcccagga tagcggccag
      721 ggggttcttc aggtcgtgcg acacgaccga gagcagttcc tcgcggatgt gcaccgcttc
      781 ctcggcccgc tgcctggcct cttcgagacg gcggttggcc tcctgtagct cggccgtgcg
      841 ctcggccacg cggcgttcca ggtcttcgtt gagccgctgt atctgctcct cggccagctt
      901 gcgcagggtg atgtcgcgca caatcacctg cacgttgccg ttgctgagca tgcgggcgct
      961 gagttccacc ggcagccggg agccgttggg acgcacaagc actcgctcgc cgataacagg
     1021 ctccccgccc tttagctcct cgaagcgcag gggcgtcttt tcgaggtcct ccggggcgat
     1081 cacctgggtc acgttaagcc ctagcagccc gtccctgggc agccccatca tctcggaggc
     1141 ccgcgagttt acttccagga tggtgccgcg ctggtcgtaa actgcgatac cgtcggaagc
     1201 ctggtctacg aggaggcgat atttggcctc gctctccatg agggcctgcc gggcgcgact
     1261 tcgctctacc acccggccca gcatagcgcc tatgttggtc atcatctcca ggagcggctc
     1321 gtccggctcc agcacctgtt tggagaagaa ttcaagcacc ccgaccattt cggtgccgag
     1381 cgtgatggga gaagcaaacg cggctcgaag ccctgctttg cgagcaaacg gcgctcggat
     1441 gaaatagggg tcgttggtca cgttgctgac ccaggccggc tttccgtggg caagcacccg
     1501 gcccggcagg ctccggcccc gaccaaaggt c
//
LOCUS       DASRWZ010000823         6554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_615699, whole genome shotgun sequence.
ACCESSION   DASRWZ010000823 DASRWZ010000000
VERSION     DASRWZ010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615699"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFH60_13045"
     CDS             <1..636
                     /locus_tag="VFH60_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314755.1"
                     /translation="FEDENNEWSEEFDVRGVKIDMSHRTVEGTERWFSEVIEGYDTDL
                     TKHDTISVIRRSYPLYGAELIERWRARTDVYPDELALRMVRQYLDWIPAVTREMGAAR
                     GELIPLYENISASERHILLVLLALNRIYLPHLAFKWTEQVAAELGIAPPNLAARLRNA
                     FLLGPEEGVRELHSLIEDTVRLVEQHMPEVDTSKVWARHRFNRGVWDRAPE"
     gene            658..2343
                     /locus_tag="VFH60_13050"
     CDS             658..2343
                     /locus_tag="VFH60_13050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6314756.1"
                     /translation="MQTSATPVIADTGPGAPLNLGLRHRRDLVNLGLLAGAFAVLLLL
                     VNPARIYPINDDWAYARAVQDLLAWNYRPPQWAQTVTISHVAWGALFAYFLGFSFTTL
                     TISTLVVSLVGLVVFYLLLRQLDIGPNFALFGVALLGCNPLYVYLSYTFMTDVTFVAL
                     MLASLLGFVLAFKQERVREGWLLVGSVACALAYLDRQFGILVCVAVLAYMWLRQEWSW
                     QRALLVAFLPALAVVSYTLWERSQPVQLISIALENANRGALANPVGLLLTKLLTITYS
                     VAMLGLCLLPLLFMRRPPRLSIVAFGLMAPFVVVLSTIEPEVLPIGNIIVSTGLITPL
                     YSRPALVPEPVWWGLNVAGTLVLCLLIGSIARATSLAWRGLLPLRRPITPGPIALVYI
                     LFGFLVPVTLVLPYSLFDRYFLPMLPLVIIAALRILQRASHSPARWRWAALVPIALFS
                     VGGMQDVSTLSMRRWYRAETLASTGVAHRNINAGFEWIGWHLYEEGEKLVREKALNTV
                     SDMPGMAVLDPVYLFSEVPQSGYEQIDETTYRVWLWGGAERRIMLLRRTGTGQ"
     gene            2340..3986
                     /locus_tag="VFH60_13055"
     CDS             2340..3986
                     /locus_tag="VFH60_13055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6314757.1"
                     /translation="MSLKWTRERRVDLLNLGLIGAAFLLVVWLVPPVRDFPMDDDWVY
                     AGSVQDLLRGDFQVGQWAQAIALGHEAWAALLAYFLGFSFTTLSIANLAMGLAGLALF
                     YLLLRQLGIRPNYALFGVATLGLNPMYFYLSYSFMTDVSFVTFTLAAMLCFVLAFNSP
                     AVREGWLWWGSAASALAYLNRQFGVLVPAAVLLYMWAAREWGWRRVAAVVLIPVAAAA
                     GYALWEGSQPTRLVLLLIEQQRAYAFENPGQAALLRVFEIVYTVAIPGLCLLPLLFLR
                     VRPRYPLVAFAVLAALQVYVLRASGTVLPVVGNVVDHSGFFSSYEALWPQWIWAGIAL
                     LGGLTLCLFVGSLMSGIRPGAIRDAVRGKAEAGRTVEGDPAHIAYILLVLLVPATLFL
                     PTMLFDRYFLPMFPLLIFAALRYLQRSAPSPPWWRWALLVPFALFSLIGMRDLFEFYD
                     LRWERAEALVARSGVAHRNIDAGYEWVGWHLFDEAEPLVRQSGDYSNMIVPGRAALDP
                     VYRFSNMPIEGYEQVDSLAYRYWLAGGLERRLLLLKRSGD"
     gene            4072..4608
                     /locus_tag="VFH60_13060"
     CDS             4072..4608
                     /locus_tag="VFH60_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET6314758.1"
                     /translation="MAATPPATFRRRDFGDPPPLTPRPMSASLSVISQVMLPNDANPM
                     GNVHGGSIMRMVDIGAAVAAMRHCGRQVVTVALDHMSFLEPVFIGDLVTITSQVEYVG
                     STSMLVRVTVDAENPGSGRKVHTSSCVLTFVALDENNRPVPVPPLLTETPEEEERMAE
                     GKRLYERAKAERNRQAQV"
     gene            complement(4702..6462)
                     /gene="polX"
                     /locus_tag="VFH60_13065"
     CDS             complement(4702..6462)
                     /gene="polX"
                     /locus_tag="VFH60_13065"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HET6314759.1"
                     /translation="MPEMAPEAAEFTNATFARIFYEIAAMVELKGESPFKSRAYRTAA
                     DTFANLGEDVKQVWREGRLDDLPGVGKAISEKVDELMRTGKLRFYDRLTAEIPPALIA
                     ITEIPHVGAKTAMLLYKTLGIQGIADLEKALDDGRILQVPGIGQKSVETIRQGIATIQ
                     RRSADKRELLGTALPIMRQMLVLLKDKCPGNIDRIEVAGSARRWRPTVGDLDILATAK
                     DPIQVIDCFVKLPMVGSVESQGENKATVRLHNGLQVDLYVLPPEHYISLLNHFTSARP
                     HNIKLRDRAIRMGYHLNEYGLTKNGQPVPISSEADIYSALGLQYIPPEMREDRGEIEA
                     AERHALPTLVEMEDIKGDLHCHTTWSDGTLSVYDMARAAKARGYQYVAITDHSQSLTV
                     ANGLTVERLRAQRKEVEEARARLGPDFRIFHGIELEIKLDGTLDFSDDVLAWLDIVVA
                     SIHSGLRQPGEQVTQRALNAVRNPHVDILGHPTGRIINQREPSAMDVGAVIKEAARTG
                     TALEINSAPDRLDLDDIYIKEAMEEGCTLSIDTDAHSRGNYDLLEYGVHLARRGWATP
                     DRVLTCWDGDKVEAWLKARG"
BASE COUNT         1043 a         1941 c         2145 g         1425 t
ORIGIN      
        1 ttcgaggacg agaacaacga gtggtcggag gagttcgacg tgcgcggcgt caagatcgac
       61 atgagccacc gcactgtgga gggtaccgag cgttggttct cggaggtcat agagggctat
      121 gacaccgacc tgaccaagca cgacaccatt tcggtgatac ggcgctcgta tcccttgtac
      181 ggggccgagc taatcgagag gtggcgcgcc cgcaccgatg tttaccctga cgagcttgcc
      241 ctgcgcatgg tgcgccagta cctcgactgg atcccggctg tgacccgcga gatgggggcg
      301 gcacgcggcg agctgatacc cctctacgaa aacatctcgg cttccgagag gcacatttta
      361 ctggtgctgc tcgccctcaa ccgcatctac ctgccgcacc tcgccttcaa gtggaccgaa
      421 caggtggcag ccgagctagg catcgccccg cccaatctcg ccgcccgcct gagaaacgcc
      481 ttcctcctgg gaccggaaga gggcgtgcgc gaactgcact cgctcatcga ggataccgtc
      541 cgcctggtcg agcagcacat gccggaggta gatacgtcaa aggtgtgggc caggcacagg
      601 ttcaaccggg gtgtgtggga ccgcgcccct gagtagtggt tggactataa tcgaggcatg
      661 cagacatcag caacgccggt tatcgccgac accgggccag gtgcgcccct gaacctgggc
      721 ttgaggcacc gcagagacct cgtcaacctg gggctgcttg ccggtgcttt cgccgtcctg
      781 ttgctgctgg taaaccccgc tcgcatatac cccataaacg acgactgggc ttacgcacgc
      841 gccgtccagg acctgctcgc ctggaactac agacctccac agtgggcgca gaccgtcact
      901 atcagccacg tagcctgggg ggccttgttt gcctacttcc tcggtttcag ctttaccacg
      961 ctcacgatct ctacgttagt ggtgagcctg gtggggctgg tcgtatttta tcttctgcta
     1021 agacagctag atataggccc caacttcgcc ctgtttggcg tcgcactgct gggctgcaac
     1081 ccactgtacg tttacctgtc ctacaccttc atgaccgatg tcacttttgt ggcattgatg
     1141 ttggcctcgt tgcttggctt tgtgctggct ttcaagcagg aacgggtgcg cgaggggtgg
     1201 ctcctggtgg gtagcgtcgc gtgcgcgctg gcctacctgg accgacagtt cggcatactc
     1261 gtttgtgtgg ccgtgcttgc gtacatgtgg ctgcggcagg agtggtcctg gcagcgtgcg
     1321 ctcctggttg cgttcctgcc tgcgctggcg gtggtaagtt acacgctctg ggagcgctcg
     1381 cagccggtgc aattgatctc cattgctctt gagaacgcga accggggtgc gctggccaac
     1441 ccggtaggct tgctcctgac gaagttgttg accataacct attcggtcgc tatgctcggc
     1501 ctgtgtctgc tgcccctgct gttcatgcgc aggccgccgc gcttatcgat tgtagctttc
     1561 gggttgatgg cacccttcgt agttgtactc tccacgatcg agccagaggt gctgccgatt
     1621 ggcaacatta tcgtctccac ggggctgata acgcccctgt attccaggcc ggccctggtg
     1681 ccggagccgg tctggtgggg gttgaacgta gccgggactc ttgtactctg cttgctgata
     1741 ggttccatag cgcgtgctac gtcgttagcc tggcgcgggt tgttgccatt gcgacgaccg
     1801 ataacgcccg gccccatagc tctagtctat attctgttcg gctttttggt ccccgtaacg
     1861 ctcgtgcttc cttactctct attcgaccgc tatttcctgc cgatgttgcc gctggtcatt
     1921 atcgctgcgc tgcgaatatt gcaacgcgcc tctcattcgc ccgcccggtg gcgctgggct
     1981 gctctggtgc ctatcgcgct gttcagcgtg ggcggcatgc aagacgtatc gacgctttcg
     2041 atgcgacgct ggtatcgtgc cgaaacgctg gccagtactg gtgtcgcgca ccggaatatc
     2101 aacgcgggtt tcgaatggat cggctggcac ctgtacgaag aaggtgagaa gctggtgcgc
     2161 gagaaggcgc taaatacagt gtctgatatg cctggcatgg cggtgctcga cccggtctac
     2221 ctgtttagcg aagtgccgca gtcggggtac gaacagatcg atgagacgac gtaccgggtg
     2281 tggctgtggg gcggcgcgga gaggcgcatc atgctcctga gacggacggg taccggccag
     2341 tgagcctcaa gtggacgaga gagcggaggg tagacctgct aaacctgggt ctgattggcg
     2401 ctgccttcct gctcgtggta tggctggtcc ctcccgtgcg tgacttcccc atggatgacg
     2461 attgggtgta cgccgggtcg gtgcaggacc tgctcagggg cgacttccag gtaggacagt
     2521 gggcgcaggc gattgccctg ggccatgaag cgtgggcggc gttgttggcc tactttcttg
     2581 gcttcagctt taccacgctc agcatcgcta acctggcgat gggactggcg ggcctggcgc
     2641 ttttctacct gttgttgagg caactcggca tcagaccaaa ttacgcgctg tttggcgtgg
     2701 cgacgctcgg cctgaaccct atgtacttct acctgtcgta cagctttatg accgacgtat
     2761 cgtttgttac tttcacgctg gcggccatgc tttgcttcgt gcttgccttc aactcgcctg
     2821 cggtgcgcga gggctggctc tggtggggga gcgccgccag cgctctcgcc tatctgaatc
     2881 gccagttcgg cgtgctcgtt cccgcggcgg tgttgctcta catgtgggcg gcacgtgagt
     2941 gggggtggcg gcgggtagcc gcggtggtgc tgattccggt ggccgcggcg gcagggtacg
     3001 cgctctggga aggctcgcag ccgactcggc tggtattgct gttgattgag caacagcggg
     3061 cctacgcctt cgagaatccc ggccaggccg cgctgctgcg ggtgttcgag attgtctata
     3121 cggtagccat accggggttg tgcctgctgc ccttgctgtt cctcagggtc cgcccgcgct
     3181 acccacttgt cgcgttcgct gtgttggcgg cgctgcaagt gtacgtcctg cgggccagcg
     3241 gcaccgtgtt gcccgtggtg ggtaacgtcg tggaccacag cggcttcttt tcgagctatg
     3301 aggctttgtg gccgcagtgg atatgggcgg gcatcgctct gttgggcggc ctgacgctct
     3361 gcctgttcgt tggctctctc atgtcgggca tccgtccggg ggccatacgt gatgctgtgc
     3421 gcggaaaggc agaggcgggc cgtaccgtag agggagaccc ggcacatatc gcttatatac
     3481 tacttgtgtt gctcgtcccc gcgacccttt tcttgccgac gatgcttttc gaccgttatt
     3541 tcctgccgat gttcccgctg ctgatattcg ctgctttgcg gtacctgcaa cgctcggcac
     3601 cctctccgcc gtggtggcgg tgggccttgc tggtgccatt tgccttgttc agcctgattg
     3661 gcatgcgcga cctgtttgag ttctacgacc tgcgctggga gagggcagag gcgctggtgg
     3721 cgaggagcgg agtggcccac cgcaatatcg acgcgggcta cgagtgggtg ggctggcacc
     3781 tgtttgacga ggcggagccg ctggtacgcc agagcggcga ttactccaat atgatcgtgc
     3841 ccggacgggc tgccctcgac ccggtctatc gcttcagcaa catgcccatc gaaggctacg
     3901 agcaggtaga ctccctggcg tacaggtact ggctcgcggg cgggctggaa cgacggctgc
     3961 tgctgctgaa acggagcggt gattagcatg ctgcgcggcg aaataacagc ataacagtta
     4021 caatatagag agtatagatt ttccagggac tgacagtaaa aggagcacct aatggcagcg
     4081 accccgcccg ccacattccg caggcgcgat tttggagacc caccccctct cactcccagg
     4141 cccatgtcgg cctcactcag cgtcatcagt caggtgatgc tgccgaacga cgccaaccct
     4201 atgggcaacg tgcacggcgg ctcgatcatg aggatggtgg atataggcgc tgccgtggct
     4261 gccatgcggc actgcgggcg gcaggtggtc accgtggcgc tggaccacat gagcttcctg
     4321 gagccggtgt tcataggcga cctggtgacg attacctccc aggtggagta cgtgggcagc
     4381 acctccatgc tggtgcgcgt gaccgtggac gccgagaacc ccggttcggg ccgcaaggtg
     4441 cacacaagct cctgcgtgct caccttcgtg gccctggacg aaaacaaccg ccccgtgccc
     4501 gtgccgcccc tgctgacgga gaccccggag gaggaagagc gcatggccga gggcaagcgc
     4561 ctgtacgagc gtgctaaagc ggagcgtaac aggcaggcgc aggtctaaca cgtaaagcgt
     4621 aacacgtaaa gcgtaacacg tattttgcct tgccgccagt tacgtgttac gctttacgtg
     4681 ttacgtgtta ctttccacgc ttcagcctct cgccttcagc cacgcctcta ccttgtcgcc
     4741 gtcccagcag gtgagcacgc ggtcgggggt ggcccagccc cggcgggcga ggtggacgcc
     4801 gtactccagg aggtcgtagt tgccccggct gtgcgcatcg gtgtcgatcg agagggtgca
     4861 gccctcctcc atcgcctctt tgatatagat gtcgtccagg tcgaggcggt cgggggccga
     4921 gttgatttct agcgcggttc cggtgcgggc ggcctccttg atgaccgcgc ccacgtccat
     4981 agccgacggc tcgcgctggt tgatgatgcg gcccgtgggg tggcccagga tgtccacgtg
     5041 ggggttgcgc accgcgttga gggcgcgctg ggtgacctgc tcgcccggct gccgcaagcc
     5101 gctgtggatg gaggccacca cgatgtcgag ccaggccagc acgtcgtcgg agaagtcgag
     5161 cgtgccgtcg agcttgatct caagctctat gccgtggaag atgcggaagt cggggcctag
     5221 cctggctcgg gcctcctcta cctccttgcg ctgggcgcgc aaccgctcca ccgtgaggcc
     5281 gttggcgacg gtgaggctct ggctgtggtc ggtgatggcg acgtactggt agcccctggc
     5341 tttggcggcg cgggccatgt cgtacacgct gagggtgccg tcgctccagg tggtatggca
     5401 gtgcaggtcg cccttgatgt cctccatctc gaccagggtg ggcagggcgt ggcgctcggc
     5461 ggcctcgatc tcgccccggt cctcccgcat ctccggcggt atgtactgca agccgagcgc
     5521 gctgtaaata tcggcttcgc tgctgatggg cacaggctgc ccgttcttcg tgaggccgta
     5581 ctcgttcagg tggtagccca tacgtatcgc ccggtcgcgc agcttgatgt tgtgggggcg
     5641 tgcgctcgtg aagtggttga gcagcgagat atagtgttcg gggggcagca cgtagaggtc
     5701 cacctggagg ccgttgtgca ggcgcacggt ggccttgttc tcgccctggg attctaccga
     5761 gccgaccatg ggcagcttca cgaagcagtc gatcacctgg atagggtctt tagcggtggc
     5821 gagtatgtcg aggtcgccca cggtgggccg ccagcgccgg gccgagcctg ctacctctat
     5881 ccggtcgatg ttgccggggc acttgtcttt gagcagcacg agcatctgcc gcatgatagg
     5941 gagggccgtg ccgagcaact cccgcttgtc ggcgctcctg cgctgtatcg tggcaatgcc
     6001 ctggcgtatc gtctcgacgc tcttctggcc gatgcccggc acctggagga tgcggccgtc
     6061 atcgagggcc ttctccaggt cggcaatgcc ctggatgccg agcgtcttgt agaggagcat
     6121 ggcggtcttg gccccgacgt gcggtatctc ggtgatggcg atgagggcgg gggggatttc
     6181 ggcggtgagg cggtcgtaga agcgcagctt gccggtgcgc atcagctcgt ccaccttctc
     6241 gctgatggcc ttgccgaccc ccggcaggtc gtccagcctc ccctcgcgcc acacctgctt
     6301 gacgtcttcg cccaggttgg cgaaggtgtc ggcggcagtg cggtaggccc gcgacttgaa
     6361 gggcgactcc cccttcaact ccaccatcgc tgcgatctcg tagaagatgc gcgcgaacgt
     6421 ggcattggta aactccgcag cttcgggtgc catctccggc attgtacctc tcctgttatt
     6481 gataagagac gaaaacgcaa agacgcaaag aacggatagg acgagcaaag aggatgatat
     6541 ggttctggat gtat
//
LOCUS       DASRWZ010000824         4609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_1163519, whole genome shotgun sequence.
ACCESSION   DASRWZ010000824 DASRWZ010000000
VERSION     DASRWZ010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163519"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFH60_13070"
     CDS             <1..509
                     /locus_tag="VFH60_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET6314760.1"
                     /translation="YESLPLKFMIVGTVFYFITCIQGPLQAIQGFNRLIHFTNWIVGH
                     AHLALLGTFSFWAMGAIYYIIPVTLRRRIYSPGLAEVQFWLVTAGFLLMMLSLQIGGL
                     IQGAMWLNGDSQYKVLPELKPYLVIRAVSGAMIVLSGILQAWNIYKTVTASQPMTAAA
                     PAPAEAPN"
     gene            629..1816
                     /locus_tag="VFH60_13075"
     CDS             629..1816
                     /locus_tag="VFH60_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HET6314761.1"
                     /translation="MDGRTVFFGAFIVFASIIFIVVALPYLTFQPPRSADARPLSPEE
                     EAGRRLYAANGCVYCHSQYLRPQDWTGVQSFRTVGRVAQAGDYYYQQTLLLGTERTGP
                     DLSQEGGAHDDEWHLAHFYNPRYTSPKSIMPSFSFYYDGFRPKDDVLHLIAYVQSLGG
                     QAGVERAERQRALKIKLQTERARGNEAIIHEWFPSTWRNVRNPMEPTTRSLVHGKQVF
                     TTNCIGCHGLNGDGKGPANRFIANPAPRNFTDASQQLYFSDGELYDAILFGVDGTAMP
                     AWGDVLTVNDIWDVTNFIRTIPNGGLQVDQLDASMMVSPADVIPINATPLPIEPVQGN
                     VPVVTPEGGLGETGGATGANASPQPSDDETPGANVTVGPQRTAGPGGGSNATVGPAGT
                     PQP"
     gene            1887..2288
                     /locus_tag="VFH60_13080"
     CDS             1887..2288
                     /locus_tag="VFH60_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314762.1"
                     /translation="MRKHLRLMKLSWGVSALLTLAVPTIASAHEAIGDDELAVANWML
                     VGAMFSVVLGLLFGYWAFRSGQFSNVEESKFTMLDMAEDYEAIMSESDAREAAILREE
                     QRSGTKPTAGTKAANSSPGAGGEPATRPTGI"
     gene            2319..2615
                     /locus_tag="VFH60_13085"
     CDS             2319..2615
                     /locus_tag="VFH60_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314763.1"
                     /translation="MSFPNEDDLIQPWSWNLPLEAVMWFIVLLLAAGIWLWTSSGKDR
                     KNLPDVLGRNVEDFAGVTQEGNGPIPLFLLMFYLVVALFMIGYPIVTLIFNYDY"
     gene            2691..3011
                     /locus_tag="VFH60_13090"
     CDS             2691..3011
                     /locus_tag="VFH60_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314764.1"
                     /translation="MEHEARLTQDSIQQAHELRSVVTHEPLYGLAPEPGQGGPESAAG
                     DDSQPKIEAPVEEPASGADHRLNMTWGIILGGVLLLFIVAAVLYLGIGNTYSSSPFTQ
                     YPLK"
     gene            3024..3338
                     /locus_tag="VFH60_13095"
     CDS             3024..3338
                     /locus_tag="VFH60_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314765.1"
                     /translation="MAFVRTISFMVPSERTQELEPGHNLYLAAVHGIKIAAQNTPGFH
                     RGGVWMQRQPDGAIRVFIYSQWYELDNIENYTSIPMVRDFERDVSTYLSTPVVDIYEV
                     VG"
     gene            3390..3944
                     /locus_tag="VFH60_13100"
     CDS             3390..3944
                     /locus_tag="VFH60_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314766.1"
                     /translation="MDPHASAVLRWRKPVYIVLGILGLAMFIMSLVQVWVLLTVEDPS
                     GFGAVQADSEGMPATDQRGAYLMLKPDHDPAFPSGLIKLLTTTQAGVIDPTVGAHVTP
                     ADLDAVLLESSNISTEVSDYRLYIIADQVEYPMKYDRATGGKSLQIRPASGVWRPGAY
                     QLDVPSDGMFGGRDYYQFYVDAAE"
     gene            3984..>4609
                     /locus_tag="VFH60_13105"
     CDS             3984..>4609
                     /locus_tag="VFH60_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET6314767.1"
                     /translation="MATITAQQSLAGTRPTPPTEDDAQTQHDYDHGIARFFYAAAAAL
                     ILSAVQGVAQRLPGVSDWLRDADYGGHMVTNLAQTHITIVGAGTISLTGLIYYVLPRV
                     CRRPLYSQALTNVSFWATLIGVFGFYIAMLSVGSYEGAMVHAGWPYDAARDWMGALHK
                     APMAMTAAVMGIGYWTFVTNVYVTVAKASSERRANPEKGATASEFLLA"
BASE COUNT          917 a         1443 c         1410 g          839 t
ORIGIN      
        1 tttacgagag cctgccgctg aagtttatga tagtgggcac cgttttctac ttcatcacct
       61 gtatccaggg gccgctacag gctatccaag gcttcaacag gttgatccac ttcaccaact
      121 ggatcgtggg gcacgcacat ctcgctttgc tcggcacctt ttccttctgg gcgatgggcg
      181 cgatctatta catcattccc gttaccttgc gccgtcgcat ttacagccct ggcctcgctg
      241 aagttcagtt ctggctggtc accgcgggct tcctgctgat gatgctttcg ctgcaaatcg
      301 gcgggctgat acagggggcg atgtggctca acggcgactc gcagtacaag gtgcttcccg
      361 aactcaagcc ttacctggta atccgcgccg tctcgggtgc tatgatcgta ctgtcgggca
      421 ttctccaggc ctggaacatc tacaagaccg tcacggccag ccagcctatg acggcggctg
      481 ccccggcacc cgctgaagcg cccaactagc ccaggcacgc agtgtaacgc cgcgtagacc
      541 tgccacacca ggaaggcact cggctccaaa gcgtcaaata acaggacgag gagtgtaaat
      601 ggaccacaac ggtggagcag tattcagaat ggacgggcgg accgtgttct tcggtgcgtt
      661 catcgtcttc gcctcaatca tcttcattgt ggtggcgctg ccttacctga cgttccagcc
      721 acctcgttcc gccgacgctc gccccctgtc cccggaggaa gaggcgggcc gccggttgta
      781 cgcggccaac ggttgcgtct actgccacag ccagtacctg cggccccagg actggaccgg
      841 cgtgcagtcg ttccgcacgg tcgggcgcgt ggcccaggcc ggagactact actaccagca
      901 aacactcttg ctcggcaccg agcgcaccgg ccccgacctg tcgcaggaag gcggcgctca
      961 cgacgacgag tggcacctgg cccatttcta caacccgcgc tacacctcgc ccaagtcgat
     1021 catgccatcg ttcagtttct actatgatgg tttccggccc aaggacgacg tgctgcactt
     1081 gatcgcgtac gtgcaaagtc tcggtgggca ggctggtgtg gagcgtgcgg agcgccagcg
     1141 cgccctcaag atcaagctac agacggagcg cgcgcggggc aacgaggcca tcattcacga
     1201 gtggttcccc agcacctggc gcaacgtgcg caacccgatg gagccgacta cgcgctcgct
     1261 ggtgcacggc aagcaggtct tcaccaccaa ctgcatcggc tgccacggcc tgaacggcga
     1321 cggtaagggt ccggcgaacc gctttatcgc caatcccgcc ccccgcaact ttaccgatgc
     1381 tagccagcaa ctttacttca gcgacggtga gctttacgac gcgatcctgt tcggtgtgga
     1441 cggtacggcc atgcccgcct ggggtgacgt gctcaccgtg aacgacatct gggacgtgac
     1501 caactttatc cgcactatcc ccaacggcgg cctccaggtg gaccagctcg acgcgagcat
     1561 gatggtcagc ccggcggacg tgatccccat aaacgctacc ccgctaccga tagagcctgt
     1621 gcagggcaac gtgccggtgg tcacgccgga aggtggcctg ggagagaccg gcggcgcgac
     1681 gggggccaac gcttcgccgc agccatcgga cgacgaaaca ccgggcgcga acgtcacggt
     1741 tggcccgcag cgcacggcag gcccaggtgg gggcagcaat gccacggtag gtccggccgg
     1801 aacgccacag ccatagtgtt caactccgag gacgaaagca accaggtcaa gaacgtgcct
     1861 ggtgctcagg aagtgggtga ttggtcatga gaaagcattt gcggttgatg aagctatcct
     1921 ggggtgtgtc agctttactt acgctggcgg tccccacgat agcctcggct cacgaggcga
     1981 taggcgatga cgagctggcg gtggccaact ggatgctggt cggtgcgatg ttttcggtgg
     2041 tgctcggcct gctgtttggc tactgggcct tccggtcggg ccagttcagc aacgtcgagg
     2101 agtcgaagtt cacgatgctt gacatggcgg aggactacga ggccatcatg agcgagtcgg
     2161 acgcccgcga agctgccatt ttgagggagg agcagcggag tggcacgaag cccacggcgg
     2221 gcacgaaagc tgctaactct agccccggcg cgggcggcga acccgccacg aggccgacgg
     2281 gcatctagcc gcgcggagaa cgagggaggc aagctggtat gtcgttccca aacgaagacg
     2341 atctaatcca accctggtcc tggaacctgc ctcttgaggc ggttatgtgg ttcatcgtgc
     2401 tcctgctggc ggcgggcatc tggctgtgga ccagttcggg taaggaccgc aagaacctcc
     2461 ccgacgtgct tggccgcaac gtggaggact tcgccggtgt cacgcaggag ggcaacgggc
     2521 caatcccctt gttcctcctg atgttctacc tagtagtcgc cctgttcatg ataggctacc
     2581 caatcgttac ccttatcttc aactacgatt actgaggtgg gccgtcgcta taacgaccct
     2641 caggcgttag acgtacccac gggatagaat tcaggcacca ggagaggaat atggagcacg
     2701 aggcgaggct cacacaggac agcatacagc aggcgcacga actgcggagc gtggtgaccc
     2761 acgagcctct ctatggcctg gcaccggagc cgggacaagg ggggccggaa agcgccgccg
     2821 gggacgactc gcagccgaag atcgaagccc ctgtcgaaga gcctgccagc ggcgcggacc
     2881 accgcctgaa catgacctgg ggcataatcc tgggtggggt gcttctgctg ttcatcgtgg
     2941 ccgccgtgct ctacctcggc atcggaaaca cctactctag ctcgcctttc acacagtacc
     3001 cactcaaata gcgagggctt cgcatggcat ttgtacgcac gataagcttc atggtcccgt
     3061 ccgagcgcac gcaagagctt gagccgggcc acaacctcta cctggccgcc gtgcacggca
     3121 tcaagatagc cgcccagaat acgcccggct tccaccgggg cggcgtgtgg atgcagcgcc
     3181 agcccgacgg ggcgataagg gtgttcatct actcgcagtg gtacgagtta gacaatatcg
     3241 aaaactacac cagcataccg atggtgcgcg atttcgagcg cgacgtaagc acctatctta
     3301 gcactcccgt ggttgacatc tacgaggtag tgggctaaca tccgttccgg ggtgctgtcc
     3361 aaccgtccat gcggctagaa gggaattaaa tggacccaca tgctagcgcg gtactacgct
     3421 ggcgaaagcc cgtatatatc gtgcttggta tactggggct ggcgatgttt attatgtcgc
     3481 tcgtccaggt atgggtgctc ctcacggtag aggacccctc gggcttcggg gccgtgcagg
     3541 cggactccga gggcatgcct gctacagacc agcgcggtgc ctacctgatg ctcaagcccg
     3601 accacgaccc cgccttccca agcggcctca tcaagctgct gaccaccacg caggccgggg
     3661 tgatagaccc gaccgtgggg gcgcacgtca cgcccgccga cctcgatgca gtgttactgg
     3721 agtcctcgaa catcagcacc gaagtcagcg actaccggct ctatatcatc gctgaccagg
     3781 tcgagtaccc aatgaagtac gaccgggcaa cgggcggcaa gtctctacag ataaggccgg
     3841 caagcggcgt ctggaggccg ggggcgtacc agttagatgt acccagcgac ggcatgttcg
     3901 gcggccgcga ctactaccag ttctacgttg acgcggcgga gtagcaatct atccaacaga
     3961 catgagggaa ggttagtgga aacatggcaa cgattacggc acagcaatcc ttagcaggca
     4021 cccgccccac tccacccacc gaggacgacg cgcaaacgca gcacgactac gaccacggta
     4081 tcgcgcgctt cttctacgca gcggcggcag ccctcatcct gagcgcggtg cagggtgtgg
     4141 cccagaggct gcccggcgtc tcagactggc tgcgcgacgc cgactatggc gggcacatgg
     4201 tgaccaacct ggcccagacg cacatcacca tcgtaggggc gggcacgatc agcctcaccg
     4261 gcctcatcta ctacgtgctc cccagggtgt gtcgcaggcc gctatacagc caggcgctca
     4321 cgaacgtttc cttctgggct accctcatcg gcgtgttcgg cttctacatc gccatgctca
     4381 gcgtcggctc ctacgagggc gcgatggtgc acgccggttg gccctacgac gcggcgcggg
     4441 actggatggg cgctttgcac aaagcaccca tggcgatgac ggcggctgtt atgggcatcg
     4501 gctactggac cttcgtgacc aacgtatacg tgacggttgc taaggcttcg agcgagcggc
     4561 gggccaaccc ggagaaaggc gctaccgcct cggagttcct gctcgccaa
//
LOCUS       DASRWZ010000825         5083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_272292, whole genome shotgun sequence.
ACCESSION   DASRWZ010000825 DASRWZ010000000
VERSION     DASRWZ010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272292"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..871
                     /locus_tag="VFH60_13110"
     CDS             152..871
                     /locus_tag="VFH60_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HET6314768.1"
                     /translation="MPISGTLLNVLTVLVGGTMGLLLGGRLPERFHGIIFNGLGLATL
                     LIGMQNALTTANILVLLGGILVGGLIGEALRLDYHLDNLGNYLQKRLAGNPDRKGSAT
                     FSEAFVTSSLIFCVGPLTILGSIQNGISGDITFLAIKSLLDGFAAFAFAASLGWGVLV
                     SAVTVLVYQGALSLVAWVAMSGVPSRDNPYIVEMTAAGGLIIIGVALRLLNIKELKLA
                     NYLPALAVAPAIVWVLQHWPW"
     gene            953..1219
                     /locus_tag="VFH60_13115"
     CDS             953..1219
                     /locus_tag="VFH60_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314769.1"
                     /translation="MLIPVAALLIFVVAVVGLLFGERLGWMDMLRQIPPIAIFALVAF
                     ALLGGWRMRPGRSIGTMGRNIWLALFIFSVLVLVWQVVSYFQAQ"
     gene            1594..2154
                     /locus_tag="VFH60_13120"
     CDS             1594..2154
                     /locus_tag="VFH60_13120"
                     /inference="COORDINATES: protein motif:HMM:NF025108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HET6314770.1"
                     /translation="MFKKHLKVSVAAMCVMAALVPTAAFAADTTVTVNGGALSISAPT
                     VATFTAVNMDGTAKETNAAVGTFSVTDARGNGNGWHVTASASQFAEWSGTAYVTDVKT
                     LAAGSLELSEPSATANGTDSPAPTVSSGPYTIDSGSGVSVASAAVDEGMGKYDFGATS
                     LTLSIPADAYARTYKSTVTISVTTAP"
     gene            2232..3347
                     /locus_tag="VFH60_13125"
     CDS             2232..3347
                     /locus_tag="VFH60_13125"
                     /inference="COORDINATES: protein motif:HMM:NF017810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HET6314771.1"
                     /translation="MRVRNIIAVAALLACLHTVLALPATAQEGAEVSDFSLTAVSDAQ
                     QPLVTEKGYFIYQLAPGSTSAGKLLVVNSGDKPLEVQLAVVPAMTAQNGGSTFGAPGS
                     GSGGPADWVTLDRASVSLEPGASSTVGFVVRVPGAVQPGQYLAGVAAYKPKTEVTKQI
                     SNGQNQASAILDVQMRYVVAVQVDVPGAWQAAMSIPEVSVMEQPSGAFLGVHLKNSGD
                     MLLRPTGTVKVADESGNTIVERAINMGTFVTGTEVRYPVPWPAIPKPGSYSVRVVLNY
                     GDGKTTTYDQVMRVEAPNAVPALPAADSRNAPDPEANSSADNLATNAAPAQAAGAGVV
                     LVAIEPWMLYGFGFVLLAIVVLLALNLAAGRRRQKAE"
     gene            3508..4596
                     /gene="dprA"
                     /locus_tag="VFH60_13130"
     CDS             3508..4596
                     /gene="dprA"
                     /locus_tag="VFH60_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET6314772.1"
                     /translation="MHPQTPYYIALSKVPGVGAARVRRLLDYFGSVETAWHATLGDLL
                     ASGLDARSAMSLVETRRTASVESDLERLAKLGGRALSWEDDEYPQRLKETGSMPPVLY
                     MLGEVTPQDAWAVGIVGTRRATHYGKEATERITAGLVEAGVSVVSGLARGIDTVAHRA
                     ALEAGGRTLAVLGSGLDVVYPPENRNLARQITEEGLGAVLTEYPLGTQPDAVNFPPRN
                     RIISGLSLGVLVVEAAEKSGALITVEFALEQGRDVFAVPGPLTSRLSEGTNSLIKKGA
                     KCVTQAADILEELHLNMVTEHVAAVRALPSDPTERMLLAHLQDDPQHIDHLTNLSGLP
                     ASTVSAVLTMMELKGLVRCIGGMQYAAR"
     gene            complement(4728..>5083)
                     /locus_tag="VFH60_13135"
     CDS             complement(4728..>5083)
                     /locus_tag="VFH60_13135"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HET6314773.1"
                     /translation="ARGERYMLIGFDDKTGAFVKSVDPSITKDRIEDLLSAYTEPVPS
                     VRYDTTTWEDGMVGLIEVIREAHQVPYRVKVNIGDKLKAGDVFVRRGSHTYRLTTDEV
                     EYLDLVAEGDRARLT"
BASE COUNT          969 a         1724 c         1540 g          850 t
ORIGIN      
        1 ctcgctcatc ctgtatattg aggactctgg cctacccacc tgatccttcg ctacgctcag
       61 gatgacaggg gggagtaggg tcccacgccc caacttcgcc cgcttacgaa ccggcaaccg
      121 caaaccgcag gaagcttcaa aaaggagacc catgccaata tccggcacac tgctcaacgt
      181 gctgaccgtc ctggtgggcg ggacgatggg cctgctcctg ggcgggaggc tgcccgaacg
      241 cttccacggc atcatcttca acggcctggg cctcgccacc ctgctcatcg gcatgcagaa
      301 cgccctcacc accgccaaca tcctcgtgct gctcggcggc atcctggtgg gcggcctcat
      361 aggggaggcg ctacgccttg actaccacct ggacaacctg ggcaactact tgcagaagcg
      421 cctggcgggc aatcccgacc gcaaaggctc ggccaccttc agcgaggcgt tcgtcaccag
      481 cagcctcatc ttctgcgtgg ggccgctcac catcctcggc tcgatacaga acggcatcag
      541 cggcgacatc accttcctgg cgatcaagtc gctgctcgac ggcttcgcgg ccttcgcctt
      601 cgcggcaagc ctgggctggg gcgtgctggt gtcagccgtt accgtgctgg tataccaggg
      661 cgcgctcagc ctggtagcct gggtagccat gagcggcgtc ccctcccgcg acaaccccta
      721 catcgtggag atgaccgccg ccggtggcct catcataatc ggtgtggccc tgcgcctgct
      781 caacatcaag gagctgaagc tggctaacta cctcccagcc ctcgccgtag cccccgccat
      841 agtctgggtc ctgcaacact ggccctggta agaacgtatt gtgcatagag agggatgggt
      901 atccgcaatc ctaaatccta aattacgaag gtctgccatg cttggaaaag atatgctaat
      961 tccggttgcc gcgctgctga tatttgtggt tgcggtggta gggctgctgt ttggcgagcg
     1021 cctgggctgg atggacatgc tcaggcagat accgcccatt gccatattcg cgctggtggc
     1081 cttcgcgttg ctgggcggct ggcggatgag gccgggccgc agcataggga cgatgggtcg
     1141 caacatctgg ctggccctgt ttatcttttc cgtgctggtc ctcgtctggc aggtcgtttc
     1201 ctatttccag gcgcaataac cggctgtacg cgccacactc tcccccttag taccccctcc
     1261 ccatagcata gaaggccccc tctagcggac cccggtcccc agtgagccgg gactgtttgt
     1321 catgcccaag aggaccacgg gcattcgcaa cctgggcact gaagccattt acttagggac
     1381 ctaagcatct tatccgcgcc cggccacgcc cctataatag cctcgaacgc aacacccaat
     1441 catctgtagg ccccgacgcg gacgctatag tacggggtgg agagaggcgt ctgcagggct
     1501 tttctctaca cgagtggtgc acaacgcgtt gccctttgcc tggacctacc ggcaagcttt
     1561 attccaatat tcttcaatag gagttatcca acaatgttca agaagcacct caaggtaagc
     1621 gtggccgcca tgtgcgtcat ggctgccctc gtacctaccg cggctttcgc agccgacacc
     1681 accgtcaccg tcaacggcgg cgcgctcagc atctcggccc ccaccgtcgc caccttcacc
     1741 gccgtgaaca tggacggcac ggccaaggaa accaacgccg ccgtgggcac cttcagcgtc
     1801 accgacgcca ggggcaatgg caacggctgg cacgtgaccg cctcggccag ccagttcgcc
     1861 gagtggagcg gcaccgctta cgtcaccgac gtcaagaccc tggccgcagg ctccctcgaa
     1921 ctgtccgagc cgagcgccac cgccaacggc accgactcgc ccgcacccac agtcagctcc
     1981 ggcccctaca cgatcgatag cggttcgggc gtgtccgtgg cgagcgccgc cgtggacgag
     2041 ggcatgggca agtacgactt cggggccacc agcctcaccc tgagcatacc cgccgacgct
     2101 tacgccagga cctacaagag caccgtcacc atcagcgtca ccaccgcacc ctaaccgggg
     2161 cgctagcgca gggggaccgc taaccggcgg tccccctgct tccgtccgaa gaacaggggg
     2221 agaagagaga catgagagta cgcaacatca tcgcggtcgc tgccttactg gcctgcctgc
     2281 acacggtcct ggcgctgccc gccaccgccc aggagggtgc cgaggtaagc gacttttccc
     2341 ttacggcggt aagcgacgca cagcagcccc tcgttaccga gaagggttac ttcatctacc
     2401 agttggcccc cggcagcacg agcgcaggca agctgctggt cgtgaacagc ggcgacaagc
     2461 ccctggaagt gcagcttgcc gtcgtccccg ccatgaccgc ccagaacggc ggcagcacat
     2521 tcggcgcgcc cggcagtggc agcggaggcc ccgccgactg ggtaacgctc gaccgcgcca
     2581 gcgtcagcct ggagccgggc gcgtccagca ccgtgggctt tgtcgtgcgc gtgcccggtg
     2641 ccgtacagcc ggggcagtac ctggcgggcg tagccgctta caagcccaag accgaagtca
     2701 ccaaacagat aagcaacggc cagaaccagg ccagcgccat actcgacgtg cagatgcgct
     2761 acgtggtagc cgtgcaggtg gacgtaccgg gcgcgtggca ggccgccatg tccatccccg
     2821 aagtcagcgt catggagcag ccgtcgggcg cgttccttgg cgtgcacctc aagaacagcg
     2881 gcgacatgct gctgaggccc accggcacgg tgaaagtggc cgacgagagc ggcaatacga
     2941 tagtcgagcg agccatcaac atgggcacct tcgtcacggg caccgaggtg cgctaccctg
     3001 tgccctggcc cgccatcccg aagccgggca gctacagcgt acgggtggta ttgaactacg
     3061 gcgacggcaa gaccaccacc tacgaccagg tcatgcgagt ggaagccccg aacgcagtcc
     3121 cagcactccc tgctgccgac tcccggaacg ccccggaccc cgaagcaaac agcagcgccg
     3181 acaacctcgc cacgaatgcg gcaccggcgc aggctgcggg cgcgggcgta gtgctggtgg
     3241 ctatcgagcc gtggatgctg tatggcttcg gctttgtgct gctggcgatt gtagtgctac
     3301 tggccctcaa ccttgccgcg ggccgcaggc gacagaaggc agaatagacc ggacgtccgg
     3361 ctacattctc atgccacagc taaatacaac acccggggag gtgggcgtgc gggcctacct
     3421 ctcttcttgt aattccactc aaattgctgt cgaaatcaac catcccggcg ggaataacca
     3481 ttagaccacc tttcccacgg accggcaatg cacccacaaa caccctacta catagccctg
     3541 agcaaagtgc ccggcgtggg cgcggccagg gtgcggcggc tgctcgacta cttcggcagc
     3601 gtagagacgg cctggcacgc caccctgggc gacctgcttg cctcgggcct cgacgccagg
     3661 tccgccatgt cgttggtgga gacgcgccgt accgccagcg tggagtccga cctggaacgg
     3721 ctggcgaagc tcggtggccg cgccctgtcg tgggaggacg acgagtaccc ccagcgcctc
     3781 aaggagacgg gcagcatgcc cccggtgcta tacatgctgg gcgaggtaac gccgcaggac
     3841 gcctgggcgg tgggcatagt aggcaccagg cgcgccacgc actacggcaa ggaggccacc
     3901 gagcgcatca cggcgggcct ggtggaggca ggggtaagcg tggtgtcggg cctggcgcgg
     3961 ggcatcgaca cggtagcgca cagggcggcg ctggaggcgg gcggcaggac gctggcggtg
     4021 ctcggctccg gcctggacgt ggtatacccg cccgagaacc gcaacctcgc caggcagata
     4081 accgaggagg gcctgggcgc ggtcctcacc gagtaccccc tgggcacgca gcccgacgcg
     4141 gtcaacttcc cccctcgcaa caggatcatc agcggcctgt cgttgggcgt gctggtggtg
     4201 gaggccgccg agaagagcgg ggccctcatc acggtggagt tcgcgcttga gcaggggcgg
     4261 gacgtgtttg cggtgcccgg ccccctcacc agccgcctga gcgaaggcac caacagcctc
     4321 atcaagaagg gcgcgaagtg cgtcacccag gctgccgaca tactggaaga gctacacctg
     4381 aacatggtca ccgagcacgt agcggcggtg cgcgccctcc cctccgaccc caccgagcgc
     4441 atgctcctcg cacacctcca ggacgacccc cagcacatag accacctgac caacctcagc
     4501 ggcctccccg cctccacggt cagcgccgtg ctcacgatga tggagctgaa gggcctggtg
     4561 aggtgtattg gcgggatgca gtatgccgcg aggtagggtt atgggtacga agctgcgcaa
     4621 actttacggg acccggccct ctccatacag cccgacccta ttccaggcct ctgagagcat
     4681 tacgcatgcg cctagatgat gcgggaggct ggtagcgccg gagcctacta ggtcagccgc
     4741 gcccggtccc cctcagcaac aaggtccaga tactccacct catccgtcgt tagcctgtag
     4801 gtatggctgc cacgccgcac gaatacatct ccggccttga gcttgtcccc aatgtttacc
     4861 tttacccggt aaggcacctg atgggcttca cgtatcacct caatcagccc caccatacca
     4921 tcctcccaag tggttgtgtc atagcgaaca gagggcaccg gctccgtgta ggcgctcaga
     4981 aggtcctcta tgcggtcctt agttatcgag gggtccacag acttcacgaa ggctccggtc
     5041 ttgtcatcga agccgatgag catgtagcgt tctccacgcg cct
//
LOCUS       DASRWZ010000826         8081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_890215, whole genome shotgun sequence.
ACCESSION   DASRWZ010000826 DASRWZ010000000
VERSION     DASRWZ010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890215"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFH60_13140"
     CDS             complement(<1..470)
                     /locus_tag="VFH60_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314774.1"
                     /translation="MRNRLACAAVLVLILCCLQLGAPRPALACYCMPPATPEEGIRLS
                     DAVFSGHIVGITMPPPTPVNGVYSPALAEFRTASVWKGVVGPNVKVTYSPSEASCGYD
                     FHAGADYLVYAHSYVDGPTLSIFGNSFQLPLGGSKLTTGICMRTRLLSTAQHDLA"
     gene            606..2240
                     /locus_tag="VFH60_13145"
     CDS             606..2240
                     /locus_tag="VFH60_13145"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET6314775.1"
                     /translation="MTSPSISPTPETILIHNARVYTMDRQASQAEAIAMREGKIVAVG
                     RTRDLEGMLGITRRIDAARRTVIPGLIDAHIHFLDYARSLALVNLEGVRSKDEALRIV
                     AQKAAELGPGRWVIGGGWNNNLWSPPDYPTRHDLDRVAPHNPVYLDRKDLHSCWVNGL
                     ALQKAGVTRDTPVPAGAAIGKDEAGEPNGMFYESARAIVRNAIDEARSDARAAMRDGF
                     RVLTGMGLVGFHSPEGPDAFHALQELDALGELKMRVVHLLAYSRLDEAIGVGLRTGFG
                     SERLRTGPVKIFSDGSLGSMTAQMLEPFEGTADNYGLATIPQEEMEAAIVRAAEAGIP
                     CAIHAIGDAANRRVLDAFEKAQPQLARLLPTHMRPRIEHAQLVHPDDIPRFAHLGVVA
                     SMQPIHATSDMHAADRLWGARSRYGYAWRSLLDAGAHIAFGSDAPVETPNPFAGIHAA
                     VTRQDEQDQPEGGWYPQERLTVEEAVRAYTEWAALSAPYLPGVTGTLTPDSAADLLVL
                     DRDIFTIPPSQIKDAHPLATIIGGEAVFDPGGIFND"
     gene            complement(2404..4110)
                     /locus_tag="VFH60_13150"
     CDS             complement(2404..4110)
                     /locus_tag="VFH60_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314776.1"
                     /translation="MADSKDDDALTKALGVAIALGALAVGVGAALANNERTRQMRAEM
                     KQRLDELGRRVDDLSSEAQKRAGGLASQAQHVIEERRPEIEQTIQRTREVVTHGLDKA
                     RTAVEQGAEKAQEYVQRVGTQAADTAGEAVDTASGVVGVSGSDLGDKAGDLAGSARDA
                     AQGAGERAGEMLEGAGDKAGDLVEGAQAGLQDAGSTVQDWTGEAGQQLSEMLSEAGER
                     ASDVREQAGSTVRDWTSTTFEAPDETGDTQDAGASSDDQVSGAGEDGQGGSQAYAADE
                     GYQGYQGTQDEQGGGGDGDGGGEGGGTQPGGYTQATLGDLGFDGNANAGASTDNPDVE
                     DANANIYGDDSAGGNASGGDAEASETSGNSDNSGNSNSAPARGIATAPLMENLGTAGL
                     DALRSDDLSTSSEQFEMSTEDVWGEGTFTEQAAGESGYQMAGSMHAHESGGSEDSRDR
                     EGNGTSGGTGATGTVEQESSDIPRIDYDLQTGGLISSGRAGWATGSELSEANGGGGAG
                     EAGAPEFGSGGGTDASESQPDFTMGEPTIPFRNDNPGSDLEVDNEPPHPGDTSTTDRD
                     RS"
     gene            complement(4297..5142)
                     /locus_tag="VFH60_13155"
     CDS             complement(4297..5142)
                     /locus_tag="VFH60_13155"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018922146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET6314777.1"
                     /translation="MSELSDKIKSTASAVRERLPADWQPDVAIILGSGLGALADQVDA
                     VAAIPYGEIPGFVQSSVQGHAGRLVLGTLEGTRVAVMQGRVHFYEGFSLQQTTFPVRV
                     LRELGAHTLLVTNAAGGLNPAFTPGDLMLIEDHINMLGWGGQNPLTGPNDPDLGPRFL
                     PMDPAYDPGLLLKAEEAARECNLDLKRGVYIVLAGPNFETRAEMRALRQWGGDAVGMS
                     TVPEVIVALHGGMKVVGISNITNMALADNTEPVNHEDVLKVAAESGPRFIGLMKALVK
                     RLRDE"
     gene            complement(5976..>8081)
                     /locus_tag="VFH60_13160"
     CDS             complement(5976..>8081)
                     /locus_tag="VFH60_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET6314778.1"
                     /translation="LVIQVTTLELFAQGLFPGLHVPFFGDNPGWLLFVDIIQFLVLVA
                     MASFFWRRLVTRPERLTDSLGALGILTAISLLMLSGLVLQGIRINLGDEPAEWRPFSI
                     AVGSLFAGLSPEVQTFIHGVAYFAHLFIVLWFLVFIVYSKHLHILTSILNVFFYDLTP
                     KGAMKPITDIEERIENEKTLGVASIKDLGWKDLVDTVTCTECGRCQDACPAWLTDKPL
                     SPKKLILDLQEHFLEAAPLELARVDKKGPLQMLAMGKLGGAEQDPGAQAEELPLVGGW
                     IMDETLWSCTTCRACMEACPVFIEHVPKITGMRQYLVMQESRFPSEFNRLFRNLEQRG
                     NPWEFPATQRDAWAAGLNVPALSDLYAQAEEEGRPLFAAPQLVTKVEREAVSHGDPAI
                     TTETEPDLENPKPKVQNPKSQEPLLEVLYFVGCLGSFDARTQRIAQSLAQVLTEAGVR
                     FGILGKEESCCGDPARRPGNEYLFQMMAQANIEVFNTYGVKKVLTSCPHCFNTLKNEY
                     PQFDGNYEVVHHSEFVNDLIKEGRIQLANKVEQAITYHDPCYLGRYNDVYDAPRAVLE
                     AIPGVELREMKRTRNNALCCGAGGGRMWLEEHVGRRMNQNRMDDVLQTGAPTLAASCP
                     FCTSMFEDGIKGKDAGDQIRLMDIAELVSLSMSRDGNGLKRGGTAPDPGPTTNADPLA
                     TGGGVGDPAKEEPPTLAQG"
BASE COUNT         1407 a         2721 c         2411 g         1542 t
ORIGIN      
        1 gccaggtcat gctgcgcggt agataacagc cgggtgcgca tgcatatgcc cgttgtcagc
       61 ttgctccccc cgaggggcaa ctgaaaggag ttgccgaaga tggagagagt cgggccatcc
      121 acgtaggagt gggcatagac caggtagtca gccccggcgt gaaagtcgta gccgcacgag
      181 gcttcgcttg ggctataagt caccttcaca ttcggcccca ccacaccctt ccaaacggaa
      241 gccgttcgga actcggccag tgcgggcgag tagacgccat ttaccggggt tggcggcggc
      301 atggtgatac ccactatgtg accgctgaat accgcatcag atagacgtat gccctcttca
      361 ggggtggcgg gcggcatgca gtagcaggca agggcagggc gtggcgctcc gagttgcaga
      421 cagcataaga tgagcaccag caccgctgca cacgcaagtc tgttcctcat ggctcacctc
      481 cttgtctcaa caaccggcgc gggtgccgaa aaatccccaa tcctaccctg cttaagccat
      541 atcttaccag aacaacacgc cttcccatgc tataatatcc ggccaatcac agcacaggag
      601 acagcatgac atcccccagc atctcaccaa cacccgaaac gatcctcatc cacaatgccc
      661 gcgtctacac gatggaccgc caggcgtccc aggcagaggc catcgccatg cgcgagggca
      721 aaatcgttgc cgtggggcgc acgcgagacc tggaaggcat gctcggcatc accaggcgaa
      781 tagacgcggc gcgccggacg gttataccgg ggctgattga cgcgcacata cacttcctcg
      841 actacgcccg cagcctggcc ctcgtcaacc tggagggggt gcgctccaaa gacgaggcgt
      901 tgcgcatcgt ggcccagaag gcagccgaac tggggccggg ccgctgggta ataggcggcg
      961 ggtggaacaa caacctctgg tcgccgcccg actaccccac ccgccacgac ctcgaccgcg
     1021 tggcccccca caacccggtt tacctggacc gcaaagacct gcatagctgc tgggtgaacg
     1081 gcctggcact gcaaaaggcc ggcgtcacgc gcgacacgcc ggtgcccgcg ggcgcggcca
     1141 taggcaaaga cgaggcgggc gaaccgaacg gcatgttcta cgagagcgcc agggctatcg
     1201 tacgcaacgc catagacgag gcgagaagtg acgcccgcgc cgccatgcga gacggcttcc
     1261 gcgtgctgac tggcatgggg cttgtcggct tccacagccc ggaaggcccg gatgccttcc
     1321 acgccttgca ggagcttgac gcgcttgggg agctaaagat gcgagtggtg cacctgctgg
     1381 catacagcag gctggacgag gctatcggcg tgggcctgcg caccggcttc ggctcggagc
     1441 ggctgcgcac cggcccggtg aagatattca gtgacggctc gctaggctcc atgaccgccc
     1501 agatgcttga gccgttcgag ggcacggcgg acaactacgg cctcgccacc atcccgcagg
     1561 aggagatgga agctgccatt gtgcgggcgg ccgaggcggg catcccctgc gccatccacg
     1621 cgattggcga tgccgctaac cgccgcgtgc tggacgcgtt cgagaaggcg cagccccaac
     1681 tcgcccggct ccttcccacg cacatgcgcc cccgcatcga gcacgcgcaa ctcgtccacc
     1741 ccgacgacat cccacgcttc gctcatcttg gcgtagtggc ctccatgcag cccatacatg
     1801 ccacctcgga catgcacgcc gccgaccgtc tgtggggcgc tcgctcgcgc tatggctacg
     1861 cctggaggag cctgctggat gccggggcgc acatagcgtt cggctccgac gccccggtcg
     1921 agacgcccaa cccgttcgcc ggcatccacg ccgccgtcac ccgccaggac gaacaggacc
     1981 aaccggaggg aggctggtac ccccaggaac ggctcaccgt ggaagaggcc gtccgcgcct
     2041 acacagaatg ggcggccctc tcagccccct acctgcccgg cgtgaccggc accctcaccc
     2101 ccgactccgc cgccgacctg ctggtgctcg accgcgacat cttcaccatc cctccaagcc
     2161 aaataaagga cgcacaccca ctcgccacca tcatcggagg cgaagcggta ttcgaccctg
     2221 gtggcatatt caacgattaa gagacgaaaa cgcaaagaac gcgcagagat taaggattat
     2281 gttctaagta caaagaagac cagggtacat gatgctgtac cctggtcctt ttgtacccac
     2341 taaacctttc tactctttgc gcgttctttg cgtctttgcg tctttgcgtt ttcgtcgtcc
     2401 ccgttacgac ctatccctat cggtggtaga ggtgtcgccg gggtggggcg gctcgttgtc
     2461 tacctccagg tcggagcctg ggttgtcgtt gcggaagggg atggtcggct ctcccatcgt
     2521 gaagtccggc tgggattcgg aagcatcggt gccgccccca ctgccgaact caggagcgcc
     2581 cgcctcccct gctccaccgc ccccgttggc ctccgaaagc tcgctgccgg tggcccaccc
     2641 tgctctaccg gaggagatca ggccgcccgt ttgcaggtcg taatcgatgc ggggtatgtc
     2701 gctactctcc tgctccacag tgccggtagc acccgtcccg ccggaggtgc cgttgccctc
     2761 cctatcacgg gagtcctcgc tgccgccgct ctcgtgagcg tgcatagagc cggccatctg
     2821 gtagcccgac tccccggctg cctgctcggt gaaggtgcct tctccccaca catcttccgt
     2881 agacatctcg aactgctcgc tgctggtaga gaggtcgtcg ctgcgcaagg cgtccaggcc
     2941 tgccgtgccc aggttctcca tcagcggggc agtagctatg cctctcgcag gggccgagtt
     3001 agaattgccg gagttgtccg agttgcctga agtctcagac gcttctgcgt cgccaccgga
     3061 agcgttgccg ccggcgctgt catctccata tatattagcg ttggcgtcct caacgtcggg
     3121 gttgtccgtg gaagcgcctg cgttcgcgtt cccgtcgaag ccgaggtcgc caagcgtggc
     3181 ttgcgtgtag cctcccggtt gggtgccccc gccttccccg cccccatcac cgtccccgcc
     3241 gcctccctgc tcgtcttgag ttccctggta gccctggtaa ccttcatcgg ccgcgtacgc
     3301 ctggctgccc ccttgcccgt cctcgcccgc gccggagacc tggtcgtccg agcttgcccc
     3361 ggcgtcctgg gtatcgcctg tctcgtctgg ggcctcgaac gtggtggagg tccaatcgcg
     3421 gacggtgctg cctgcctgct cgcgcacgtc cgacgcacgc tcgccagcct ccgacagcat
     3481 ttcggatagc tgctggcccg cctcgcccgt ccagtcctgc acggtagagc ctgcgtcctg
     3541 caaaccggcc tgcgcgccct caacgagatc gcctgccttg tcgcccgccc cctctaacat
     3601 ctcaccggca cgctcgcccg cgccctgcgc cgcgtcacgc gccgaaccgg ccaggtcgcc
     3661 cgccttgtcg cccaggtccg acccgctcac gcctacgaca ccactcgccg tatcaactgc
     3721 ctctcccgct gtgtcggcgg cctgcgtgcc aacgcgctgc acgtactcct gggccttttc
     3781 cgccccttgc tccactgccg tgcgggcctt gtcgaggccg tgcgtcacca cttcgcgggt
     3841 gcgctgtatc gtctgctcga tttcgggccg ccgctcctca atcacgtgct gggcctgcga
     3901 ggcgaggccg cccgcgcgct tctgggcctc cgacgatagg tcgtccaccc gcctgccaag
     3961 ctcgtccagc cgctgcttca tctcggcccg catctgtcgg gtgcgctcgt tgttcgccag
     4021 ggccgcgccc acaccaaccg ccagcgcccc cagcgcgata gccacaccca gcgccttcgt
     4081 caatgcatcg tcgtctttac tgtcggccat cttaatctct ccttatcagt agctggaacc
     4141 tgacgagcaa cgagtaatga ggactgagga ctgagcggcc ccgttcgaag cattagcctc
     4201 gtcgcctctt tgcacctttg cggtcctatc tcttcactca gtcctcagtc ctcagtcctc
     4261 attactcgtt actcgttact cgtcactcgt cactcgtcac tcgtcacgca atcgctttac
     4321 taacgctttc atcagtccga taaaccttgg ccccgactcg gcggccacct tcagcacgtc
     4381 ttcgtggttg acaggttccg tgttgtcggc cagcgccatg ttggtgatat tggatatgcc
     4441 tacaaccttc atgccaccat gtagggccac tatcacttcg ggcaccgtgg acatgccgac
     4501 cgcgtcgccg ccccactgcc gcagcgcacg catctcggcc ctcgtctcga agttcggccc
     4561 ggcgagcacg atatatacgc cccgcttaag atcgaggttg cactcgcgcg cggcctcctc
     4621 ggctttgagg agcaggccgg ggtcgtaggc ggggtccatc ggtaggaagc gcggccccag
     4681 gtcggggtcg ttggggccgg tgagggggtt ctggccgccc cagccgagca tgttgatgtg
     4741 gtcctcaatc agcatcaggt cgcctggcgt gaaggcggga ttgaggcccc cggcggcgtt
     4801 ggtcacgagg agggtgtgcg caccaagctc gcgcagcacg cgcaccggga aggtggtctg
     4861 ctgcaagctg aagccctcgt agaagtggac ccgcccctgc atcaccgcca ccctggtgcc
     4921 ctccagcgtg cccagcacca gcctgcccgc gtgcccctgc accgaggact gcacgaatcc
     4981 cggtatttcg ccgtatggga tggccgcgac cgcgtctacc tggtcggcca gcgcgccgag
     5041 gccagaaccg aggataatag ccacgtcggg ctgccaatca gcgggcagtc gctctctaac
     5101 ggcagacgct gtactcttga ttttgtctga tagttcgctc atgaaggttt gttcccaact
     5161 tatccattga atgctcccag acaggccctg gaactcggtc cagttcaccc gccgaaggag
     5221 tttacactcg gcataatgcg cttatcacga agtcctgcgc gcgggcctca caatctccct
     5281 ttggaaccgc tccgccaact ctcgaccttc gctttcttta cctctgggta cgggcaccca
     5341 cacttcatag ggacctctcc tgcttcctag cttctccagt tgaaacttca ggacaggtac
     5401 cgtattgtgt ggacggtctg catatccaac ccatacacgc cttagagacg caccgttacc
     5461 catcaggtgc atcaatttgc gcccgctaac tattgcaatg ggcgtaacag cgagttctct
     5521 cgtttgcgcc tttagtcctt cataccacgt ttccctcgac ttgaggaacc agacattccc
     5581 gaatgccgtt aggaagatga agcccatcac ggcaaccata cagcctgccg ccagagctat
     5641 ccaggtgcct gcgctccaaa gggagaacaa ggcgaacata cccccaccca cggacagtag
     5701 aacggtgaat aacagccaac gtgtgctcga acgccgcatt ctccgggcat ccagttcgag
     5761 gaaccgcgcc cactctccct gctcgtatgt ccactccgcc caaatgggat cgccggtcac
     5821 cgccagatcg tatgctcgat tgtaggttct gctctcgaac ataaaagcct gcctggtccg
     5881 caactccgag gtcgttagaa ctaagcctcg gcggtgatga gagcttcatt tggctgatgc
     5941 ggacggcaca acagttatgt caatgcctgg cagcgtcagc cttgcgccag ggtcggaggc
     6001 tcttcctttg ccgggtcgcc cacgccaccg cccgtcgcca gcgggtcggc gttggtggtg
     6061 ggaccggggt ccggtgcagt accaccgcgc ttgaggccgt tgccgtcgcg gctcatcgac
     6121 agcgacacaa gctccgcgat gtccatgagg cgtatctggt cgcccgcgtc cttgcctttg
     6181 atgccgtcct cgaacatcga ggtgcagaag gggcaggagg cggccagcgt gggcgcgccc
     6241 gtttgcagca cgtcgtccat gcggttctgg ttcatgcgcc gcccgacgtg ctcctccagc
     6301 cacatccgcc cgccgcccgc cccgcaacac agggcgttgt tcctggtgcg cttcatctcc
     6361 cgaagctcca cgcccggtat cgcctcaagc acggcgcggg gggcgtcgta cacgtcgttg
     6421 tagcggccca ggtagcaggg gtcatggtac gtaatcgcct gctcgacctt gttggcaagc
     6481 tggatgcggc cctctttgat gaggtcgttg acgaactcgg agtggtgcac cacctcgtag
     6541 ttgccgtcaa actgggggta ctcgtttttc agcgtgttga agcagtgggg gcaggaggtg
     6601 agcaccttct tcacgccata tgtgttgaac acctcgatat tggcctgcgc catcatctgg
     6661 aacaggtact cgttgccggg ccgccgcgcc gggtcgccac aacagctttc ctccttgccc
     6721 aatatgccga agcgtacccc cgcctcggtc agcacctgcg ccagcgactg cgctatcctc
     6781 tgggtgcgcg cgtcgaacga gccaagacag ccgacgaagt agagcacttc gagcagcggc
     6841 tcttgcgatt ttggattttg gactttgggt tttggatttt cgaggtccgg ttcggtctcg
     6901 gtggtgatgg cggggtcgcc gtgggagacg gcttcgcgct cgactttggt tacgagctgg
     6961 ggggcggcga agagggggcg cccttcttct tcggcttggg cgtagaggtc gctgagggcg
     7021 ggcacgttca ggccggcagc ccaggcgtcg cgctgggtgg cggggaactc ccacgggttg
     7081 ccgcgctgtt cgaggttgcg gaagaggcgg ttgaactcgc tagggaatcg gctctcctgc
     7141 atgaccaggt actggcgcat gcccgtgatc ttgggcacgt gctcgatgaa cacggggcac
     7201 gcctccatgc aggcccggca ggtggtacac gaccagagcg tttcgtccat gatccagccg
     7261 ccgacgaggg gcaactcctc ggcctgcgct cctgggtcct gctccgcccc acccagcttg
     7321 cccatagcga gcatctgcaa cggccccttt ttgtccaccc tggcaagctc cagcggggcc
     7381 gcttcgagga agtgctcttg caggtcgagg atcagcttct tgggcgagag gggcttatct
     7441 gtcagccagg cggggcaggc gtcctggcag cggccgcact cggtgcaggt cacggtatcc
     7501 accaggtctt tccagcccag gtctttgatc gaggccacac ccagcgtttt ctcgttctcg
     7561 atccgctcct ctatgtccgt tattggcttc atcgcgccct tcggcgtgag gtcgtagaag
     7621 aagacgttga ggatggaggt gaggatgtgc aggtgcttgg agtagacgat gaacaccagg
     7681 aaccagagca cgatgaacag gtgcgcgaag taagccacgc cgtggatgaa ggtctgcact
     7741 tcggggctaa gcccggcaaa gaggctgccc accgcgatgg agaagggccg ccactcggca
     7801 ggctcgtcgc ccaggttgat gcgtatgccc tgcaacacga ggcccgagag catgagcagc
     7861 gagatggcgg tcagtatgcc gagcgccccc aggctgtcgg tcagccgctc cgggcgcgtc
     7921 accagcctgc gccagaagaa cgaggccatc gccaccagca ccaggaactg aattatgtca
     7981 acgaatagca gccagccggg gttatccccg aagaaaggta cgtggaggcc tgggaagagg
     8041 ccctgcgcga acagttcgag ggtagtgacc tggatgacga g
//
LOCUS       DASRWZ010000827         5184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_410785, whole genome shotgun sequence.
ACCESSION   DASRWZ010000827 DASRWZ010000000
VERSION     DASRWZ010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410785"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..688)
                     /locus_tag="VFH60_13165"
     CDS             complement(83..688)
                     /locus_tag="VFH60_13165"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HET6314779.1"
                     /translation="METPQPTIFDDLRAPTGPDEEYRLASVLVLIYPRAGESYVVFMR
                     RTDTVEHHKGQISLPGGGRDATDPDPVYTALREAQEELGIDPGMVQVIGTMPDIYARV
                     SSFLITPVVALLKPGTEDRLVFKPAPEEVAEVLEVPLRVFYDESAHRTETRTHDNVTY
                     QIHFYTHGPYEVWGVTGRIMHEFTKRYPKSIFSPPQTTRNS"
     gene            complement(688..1554)
                     /locus_tag="VFH60_13170"
     CDS             complement(688..1554)
                     /locus_tag="VFH60_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314780.1"
                     /translation="MDSLKHFIKRKANGSGNGNGGASGPKGNGASSEPLPPPAKEGGH
                     GDHWGCIFRAVEQDIIISLIQDVVATDSRPDGFQAPGGGAAYHSSSSPVRVCVLVAGD
                     TLESAYPEAQHGTEWPIIVREVVPWANGVEGQVTGECHGALVSFFDTRFYANRYKYKA
                     GEQYTFRMSAFAFAVGRASDAEVEIEGGAKVSLKGARAYMPANLGNNPDADIDEYWFH
                     SPLEAPASEAAFEGKRLHALPITMAIPGHFDMSLVLYAAGHTLDPNTDLHTAEEIDLE
                     GYLWLQGYLSDV"
     gene            complement(1911..3461)
                     /locus_tag="VFH60_13175"
     CDS             complement(1911..3461)
                     /locus_tag="VFH60_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET6314781.1"
                     /translation="MSLDIEKREEELSPLELLLRGALNDATIPLGVAPTSFPFYDGEP
                     DALDYDNLLACIRCGACLPVCPTYNTDLLEVQSPRGRVQLMRQVEEGLLEPSPNFAEH
                     MYHCLDCRACQTACPTGVRIGEQVLRARVQVEKARPQPFAKKIGLDFALGDQRRLELL
                     MLPIALYQRTGLQWLVRKTRVLHWALPKRLRILAFMEELLPERIPVRSLRQQIAVTVP
                     PQGEKRYRVGFFLGCVMSTMFAQTSSATVRVLSENGCEVVTPKTQKCCGAPHAEEGDF
                     ESVKRFAKENIALFERYKVDYIICDCAACGAQTKEYAELLHDDPEWSERAHHFSAKVR
                     DIMQFLGEIPLRDSFAELSKRVAYHEACHLCHAQKVREEPVDVLRSVGVDLAPLKESD
                     MCCGSAGVYNLTHNDRSMLILDRKMDHIEATDADVVLTGNPGCLLQLDYGRRAAGSEK
                     PVLHPVQVVDAAYRAERARTGTPARFSGVPSGDDLRTKAGKMLAIAANGRTKVAFGRK
                     GAKKPPGG"
     gene            3816..4556
                     /locus_tag="VFH60_13180"
     CDS             3816..4556
                     /locus_tag="VFH60_13180"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET6314782.1"
                     /translation="MAVDVESGTQALSGKSTAFVDWMEGWLGERTPLELQSFLQGNGI
                     GPERVAVVCVDVIVGFCYEGRLSSPRIAGIVEPIARLFERAHELGVRHFLLPQEHHTE
                     DAAEFEQYGPHGIRNTREAETVDELRALPFSNTYRVFLKNSIHPALGSGFGEWLDEHP
                     SVDTFIVVGDCTDLCAHQAVMYLKLRGNAVDRRVNVLVPEDCLQTYDLPIETARQIGA
                     MPHDGDLLHAVFLYHMALNGAQVVEKIV"
     gene            4620..>5184
                     /locus_tag="VFH60_13185"
     CDS             4620..>5184
                     /locus_tag="VFH60_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314783.1"
                     /translation="MALVLAGCDNGPAPATPTLPRPAITEQIEGDITVAPTRTAQADA
                     GVGPTSTAAPGPGGVSPTEVARITPTGTIVDAVPAVTIEGPTPVDDTVPTATPVTARS
                     SGEALTALQALAELKDKASAEMPDARLAMLVNSRPGQQKILLGTSLGDPNVNEATPGG
                     LGRNWTLVAVSPSQGRAAAFSTDGTVVD"
BASE COUNT          874 a         1430 c         1878 g         1002 t
ORIGIN      
        1 aggaattgta cggcgctagc aactaattgt tcaattgggg gcctttccag cattgccacg
       61 ctcgctttcc tggtaacttt agttatgagt tacgagttgt ttgtggagga ctgaagatag
      121 atttcgggta tcgtttggtg aattcgtgca ttattcggcc tgttacgccc cagacttcgt
      181 aggggccgtg ggtgtagaag tggatctggt aggtgacgtt gtcgtgggtg cgggtctcgg
      241 tgcggtgggc tgattcgtcg tagaagacgc gcaggggcac ctcgaggacc tcggctactt
      301 cctcgggggc gggcttgaac accaggcggt cctcggtgcc gggcttgagg agggccacta
      361 cgggggtgat gaggaagctg ctgacgcggg cgtagatatc gggcatggtg ccgattacct
      421 ggaccatgcc tgggtctatg ccgagttctt cctgggcttc gcgcagggcg gtgtagaccg
      481 ggtcggggtc ggtggcgtcg cggccgccgc ctgggaggga gatctggccc ttgtggtgct
      541 ctaccgtatc ggtgcggcgc atgaagacga cgtagctttc cccggcgcga gggtagatga
      601 ggacgagcac ggaggccagc cggtactcct cgtcggggcc ggtgggagcg cgtaggtcat
      661 cgaagatggt gggctgcggt gtttccatca gacgtcgctc aggtagcctt gcagccagag
      721 gtagccttcg aggtctatct cctctgcggt gtgaaggtcg gtgttggggt cgagggtgtg
      781 cccggcggcg tagagcacga gggacatgtc gaagtggccg gggatcgcca tagtaatggg
      841 tagggcgtgc aagcgcttgc cctcgaaggc ggcctctgac gcgggggcct cgagggggct
      901 gtggaaccag tactcgtcta tgtcggcgtc ggggttgttg ccgaggttgg cgggcatgta
      961 ggccctcgcg cccttgagag acactttggc cccgccctcg atctccacct cggcatcgga
     1021 ggcgcggccc acggcgaagg cgaaggcgct catgcggaag gtgtactgct cgcccgcctt
     1081 gtacttgtag cggttggcat agaagcgggt gtcgaagaag ctcaccagcg cgccgtggca
     1141 ctcgcccgtc acctgaccct ccactccgtt ggcccagggg actacctcgc gcacgattat
     1201 gggccactcg gtgccgtgct gggcttcggg gtaggcgctt tcgagggtgt cgcccgccac
     1261 caggacgcag acgcgcacgg ggctggagct tgagtggtag gcagcgccgc ctccgggggc
     1321 ctggaagccg tcggggcggc tgtcggttgc tacaacgtcc tggatgaggc tgattatgat
     1381 gtcctgttcg accgcgcgga agatgcagcc ccagtggtcg ccgtggccgc cctctttggc
     1441 ggggggcggg agcggctcgg aggacgcgcc gttgcctttc gggccggagg caccgccgtt
     1501 gccgttgccg ctaccgttgg ctttgcgctt gatgaagtgt ttcaggctat ccaatgttac
     1561 cttccgggtc ctcccggcag ttgaaactgc gggctatgat taggaagtcc ccctgcgggg
     1621 acttgttccg acttaggcta gatgtgtcta cacaccgttc gatataggca accaaacctc
     1681 tcctgtcatc ctgagcgtag cgaaggatca gccgcccgtg tttgactctt cgcgcttgga
     1741 cacccatgtt cctgatgccg ccggcatccc cagcgttgaa gaccctggcc tacccacctg
     1801 atccttcgct gcgctcagga tgacaaggcc tctcatggtc ccgcgtacct tgcattttac
     1861 agatgctccc tggtccgcga aggcggagtt tgaaggtgta gcctgcggtt tcaaccaccg
     1921 ggcggcttct tcgcgccttt cctgccgaat gctactttgg tcctgccgtt ggcggctatg
     1981 gccagcattt tcccggcctt cgttctgagg tcgtcgccgg aggggacgcc ggagaagcgg
     2041 gcgggggtac cggtgcgggc gcgctcggcg cggtaggcgg cgtccaccac ctgcacgggg
     2101 tggagcacgg gcttttcgga gccggcggcg cggcggccgt agtcgagttg gagcaggcag
     2161 ccggggttgc cggtgagcac cacgtcagcg tccgtggcct cgatatggtc catcttgcgg
     2221 tcgaggatga gcatggagcg gtcgttgtgg gtgagattgt agacgcccgc gctgccgcag
     2281 cacatatcgc tctctttgag gggggcgagg tccacgccga cggagcggag cacgtctacc
     2341 ggctcctcgc gcaccttctg ggcgtggcag aggtggcagg cctcgtggta ggcgacgcgc
     2401 ttggaaagct cggcaaagga gtcgcgcagg ggtatctcgc ccaggaactg cattatgtca
     2461 cgtactttgg cgctgaaatg gtgggcgcgt tcggaccatt cgggatcgtc gtggagtagc
     2521 tcggcgtatt ctttggtttg ggcaccgcag gcggcgcaat cgcagatgat gtagtccact
     2581 ttgtagcgct cgaacagggc gatgttctct ttcgcgaacc gcttgaccga ctcgaagtcg
     2641 ccctcttcgg cgtggggggc accgcagcac ttttgcgtct tgggggtgac cacctcgcag
     2701 ccgttctcgc tgagcacgcg cacggtagcg gaggaggtct gagcgaacat ggtgctcata
     2761 acgcagccga ggaagaagcc gacgcgatag cgtttctcgc cctgcggcgg cacggtgacg
     2821 gctatttgtt ggcggaggga gcgcacgggg atgcgctccg gcagtagctc ctccatgaag
     2881 gcgaggatgc gcaggcgttt gggcaaggcc cagtggagca cgcgggtctt gcgcacgagc
     2941 cactggaggc cagtacgctg gtacagggcg atggggagca tcagtagctc gagccgacgc
     3001 tggtctccaa gcgcgaaatc gaggccgatt ttcttggcga agggctgggg gcgggccttt
     3061 tcgacctgca cgcgggcgcg aagcacctgc tcgcctatgc gcacgccggt ggggcaggcg
     3121 gtctggcagg cgcggcaatc taggcagtgg tacatgtgct cggcgaagtt gggggagggt
     3181 tcgagcagcc cttcctccac ctgccgcatt agctgcacgc gacctcgggg ggactgcact
     3241 tccagcaggt cggtgttgta ggtggggcag acgggcaggc aggccccgca cctgatgcag
     3301 gcgagcaggt tgtcgtagtc gagcgcgtca ggctcgccgt cgtagaaggg gaaggaggta
     3361 ggtgccacgc cgagagggat ggtagcgtcg ttgagcgcgc cccgcagcag cagttcgagc
     3421 ggggatagct cctcctcccg cttttcgatg tcgagactca tggatgaatt atatcacagg
     3481 gacgatggac gagggacggt aagagacgga accgccaaga cgccaagagc ctgccctgag
     3541 cgcagccgaa gggacgccaa gagcgcgcca agattaagaa cggttcaccg cagagacgca
     3601 gagggcgcag aggttacgca gagaaggagg tagggtgttc ttaccggggc agcatcgtac
     3661 tgtacgatat cgcacttgtc cattcccaag caataactta ctctgcgcgc cccaggtgct
     3721 ctcggtgtct gtgcggtgca cgttcggcgc tgacctgggg cgggactgtc ggttataatg
     3781 gcgtgaagtg tgaccaggca cgaggaggat gtagcatggc ggtagatgtg gagagcggca
     3841 cgcaggctct gagcgggaag agcacggctt ttgtcgattg gatggagggg tggcttggcg
     3901 aacgcacgcc actggagttg cagtcttttc tgcaagggaa tggcattggg ccggagcggg
     3961 tggcggtggt ctgcgtggat gtgatagtgg gcttttgcta cgaggggcga ttgtcatcgc
     4021 cgcgcatcgc ggggatagtg gagccgattg cgcggctgtt cgagagggcg cacgagctgg
     4081 gggtgcggca ctttttgctg ccccaggagc accacacgga ggacgcggcg gagttcgagc
     4141 agtacgggcc gcacggcatc aggaacacgc gggaggcgga gactgtggac gaattgcggg
     4201 cgctgccttt ctcgaacacg tacagggtat tcctcaagaa ctcgatacac cccgcgctgg
     4261 ggagcggctt tggcgaatgg ctggacgagc acccaagcgt cgatacgttc attgtggtgg
     4321 gcgactgcac ggacctgtgc gcgcaccagg cggtgatgta cctgaagctg cgaggcaacg
     4381 cggtagaccg cagggtgaac gttctcgtgc cggaagactg cttgcagacg tacgatctgc
     4441 caatcgagac ggcgaggcag ataggcgcga tgccccatga cggcgacctg ctgcacgccg
     4501 tgttcctgta tcacatggcc ctgaacgggg cgcaggttgt agagaagatc gtttagaagg
     4561 ctgtggaggt tgttttgagg ttacgcggca cactgtctgt tttcttgctg gtagcgacga
     4621 tggccctggt cttggcgggc tgcgacaacg gtccggcacc ggcgacgccc actctgcctc
     4681 ggccggcgat taccgagcaa atcgagggtg atataaccgt ggcacccaca cgtacggcgc
     4741 aagcggatgc cggtgtgggg ccgacctcga cggctgcgcc tgggccaggg ggcgtttcgc
     4801 ccaccgaagt tgcaagaatc acaccaaccg gcaccatagt tgacgcagtg ccggcagtga
     4861 cgatagaggg accgaccccc gttgacgaca cggtgcccac ggccaccccc gttacggcca
     4921 ggagcagcgg agaggccctg acggccttgc aggcgctggc ggaactcaag gacaaggcgt
     4981 cggcggagat gccggatgcg cggctggcga tgctggtgaa ctcgcggccc ggccagcaga
     5041 agatactgct cggcacctcg ctgggcgacc ccaacgtgaa cgaggcgaca cccggggggc
     5101 tgggacgcaa ttggacgctg gtggcggtgt ccccttcgca ggggcgggcc gcggccttca
     5161 gtacggacgg caccgtggtt gaca
//
LOCUS       DASRWZ010000828         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_411083, whole genome shotgun sequence.
ACCESSION   DASRWZ010000828 DASRWZ010000000
VERSION     DASRWZ010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411083"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..800)
                     /locus_tag="VFH60_13190"
     CDS             complement(39..800)
                     /locus_tag="VFH60_13190"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6314784.1"
                     /translation="MTADVRLVAYNVGQGGPRDRAVWERVLSDLAPDLLFVQETRPPA
                     QALLPALPTAPAGSWLWATVPGGRWGSALWTRSGYLTPLPVPEDFTGRVVAAVVEGRA
                     WPGLGVAPIVALSIHAPTRKGSSYIKEVGRILDFAGEIAGGLPLVLAGDFNVVVGLRE
                     PGQVISMTRGEQALLRRLREELDLVPCWQTAHPGEPLARTLRWLHRSDSLPYHCDGLF
                     VPAAWASALQSCEVLEAEEWLALSDHNPVVASFAL"
     gene            complement(828..1121)
                     /locus_tag="VFH60_13195"
     CDS             complement(828..1121)
                     /locus_tag="VFH60_13195"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6314785.1"
                     /translation="MANTGNIGIEVRVPTLLRDCTGGSSPVTIEAATLAEALERLRET
                     YPLLRIHLYDEAQQLRPHVLIFYNEESTRWLERLDVPLQPGDRLEIIQAVSGG"
     gene            complement(1123..2253)
                     /locus_tag="VFH60_13200"
     CDS             complement(1123..2253)
                     /locus_tag="VFH60_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET6314786.1"
                     /translation="MSGMADKARKVTVLVGTNKGAFFFHADADRRNWEMTGPHLDGWE
                     IYSLCGLNGNGHENGSSGAGNRVFAGTSHFVYGPTIRFSDDLGATWTQVEAGPSYSEE
                     SGFKLNRIWQIVPGHDTEPGTLYAGVDEAGLFASHDGGTTWEEVTGLTQHPTRDEWAP
                     GNGGLCLHTILVDPGNPRRMWVGISAVGVLRTEDGGQTWQTCNQGLPQLATGIRTQDV
                     GRCVHKLVLDPKDPNTLYCQFHGGVFKSTDAAGSWQPIEEGLPSNFGFPMGISAEGDL
                     FVVPLESDLQRHVKDGQLRVYRSRDGGAQWAPVGEGLPEQPPYVGVLRDAMAVDTLEP
                     AGVYFGTSMGEVFYSPDAGEHWDRLPGQFPRITSIKAWVREA"
BASE COUNT          437 a          838 c          833 g          442 t
ORIGIN      
        1 cgtcatgcag ggcaccccca cagggtatcc ggcggctctt acagcgcgaa cgaggccaca
       61 accgggttgt ggtcgctgag ggcgagccat tcctccgcct caagcacctc acatgattga
      121 agcgccgagg cccaggcggc gggcacgaac aggccgtcgc agtggtaggg cagggagtcg
      181 ctgcggtgga gccagcgcaa cgtgcgggcg agtggctcgc ctgggtgcgc cgtctgccag
      241 cacggcacga ggtcaagctc ctcgcgcagc cgccgcagca acgcctgctc gcctcgcgtc
      301 atggagataa cctgccccgg ctcgcgcagc cccaccacta cgttgaagtc ccccgccagc
      361 acgagaggaa gcccacctgc tatctcgccc gcgaaatcaa ggatgcgccc gacttccttt
      421 atgtaggaac tgcccttgcg cgtgggggcg tggatgctaa gggctacgat gggcgcgacc
      481 ccaaggcccg gccaggcccg cccctcgact accgcggcga ctacccggcc tgtgaagtct
      541 tccggcaccg gcagcggcgt caggtagccg gagcgcgtcc agagcgcgct accccagcgg
      601 ccgccgggca ccgttgccca tagccacgac ccggcaggcg cggtggggag cgcaggcagc
      661 aaggcttgtg cgggagggcg cgtctcctgg acgaagagca agtcgggcgc caggtctgag
      721 agtacgcgct cccagacggc ccggtcgcgc ggcccaccct gccccacgtt ataggcgacc
      781 aggcgtacgt cggcggtcac actgctatgc ccgctatgcc cgctacgtta cccaccggag
      841 actgcctgga tgatttccag gcggtcccca ggttggagcg gcacgtcgag gcgttccagc
      901 cagcgggtgc tctcctcgtt gtagaagatg agcacgtggg gccgtagctg ctgcgcttcg
      961 tcgtagaggt ggatgcgcag cagcgggtag gtctcgcgca gccgctccaa agcctccgcg
     1021 agagtggcgg cctctatcgt taccgggctg ctgccacccg tgcagtcccg caggagcgtg
     1081 gggacgcgca cttctatgcc aatgttgccc gtatttgcca tgtcaggcct cccgcaccca
     1141 cgccttgatc gaggtgatac gcgggaactg gccgggcagc cggtcccagt gctcgcccgc
     1201 atcgggggag tagaacacct cgcccatgct ggtgccgaaa tagaccccgg caggctcaag
     1261 cgtatctacg gccatcgcat cgcggagcac gccgacgtaa gggggttgct ccggcagtcc
     1321 ttcgcctacg ggtgcccatt gcgcgccgcc gtcccgcgag cggtagacgc gcagttggcc
     1381 gtcctttacg tggcgctgca agtcgctctc caatggtacc acgaaaaggt cgccttccgc
     1441 gctgataccc atcgggaagc cgaaattgct cggcaagccc tcctcgatag gctgccacga
     1501 gccggctgca tcggtggact tgaacactcc gccgtggaac tggcagtaga gcgtgttggg
     1561 gtcttttgga tcgagcacga gcttgtgcac gcagcgcccc acgtcctggg tgcggatgcc
     1621 cgtcgctaac tgcgggaggc cctggttgca ggtctgccag gtctggccgc cgtcttcagt
     1681 gcggaggacc cccaccgccg agatgccgac ccacatccgc cgggggttgc cgggatcgac
     1741 caggatggtg tgcaagcaga ggccgccgtt gcccggtgcc cactcatcgc gggttgggtg
     1801 ctgggtgagg ccggtcacct cttcccaggt ggtgccgccg tcgtggctgg caaacaggcc
     1861 cgcttcgtct acccctgcgt agagggtgcc tggctcggtg tcgtggccgg gcacgatttg
     1921 ccagatgcgg ttgagcttga agccgctctc ctcgctgtag ctgggtccgg cctcaacctg
     1981 cgtccaggta gcgcccaggt cgtcgctaaa gcggatggtt ggcccgtaaa cgaaatggga
     2041 cgtgcccgcg aacacccggt tgcccgcccc actcgacccg ttctcatggc cgttgccgtt
     2101 cagcccgcac aggctataga tctcccagcc atcgagatgc ggtccggtca tctcccagtt
     2161 ccgacggtcg gcgtccgcgt ggaaaaagaa cgcgcccttg tttgtgccga ccaatacagt
     2221 caccttgcgt gccttgtctg ccatacctga catgcctgtg tgcctccttc tagatgaaag
     2281 taccacctgg gccggagtat agcacttatg ttctagggag tcaaacggcc gccacgctgt
     2341 gtattcgcca ctcgcaaagc gcacgatggc atcagggtgt aagcatccca ggcagggaaa
     2401 ctatgtcggc cctgtggcta aagccacggg ctgggaagca gcgaagtctc cttcggagac
     2461 tagggactta gctgcaaagt ggaggaagca aacgaacgaa ggtggcccct gtcattctga
     2521 gcgtagcgaa gaatctagga tgctggcatg
//
LOCUS       DASRWZ010000829         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_684249, whole genome shotgun sequence.
ACCESSION   DASRWZ010000829 DASRWZ010000000
VERSION     DASRWZ010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684249"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VFH60_13205"
     CDS             complement(<1..1230)
                     /locus_tag="VFH60_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314787.1"
                     /translation="MSKGEDRVPTRRIVLVLGLLLGVVMTAALLRLQANRQDNNVSST
                     GQGTTTAANGLIGEARGIIAPGQDPQDEIEILRSREEYFRTRYGLPEQQIDNNWLVAA
                     EKQVARMQSGIPAGKISYDRSQADSPLVLDPGQLISLGPRPGQSDPCASCFKYGLVSG
                     RINSIAIHPTITSTVYLGVSNGGVWKTTTCCTLNTQWYPTTDDPSIGTQTIDEVTLDP
                     NNPDIVYAATGDFRATGAARGSNGILKSTDAGASWTVLGQSVFPPFRNGPFGPGQPIG
                     GSSQYQAVSAIKVDPNNSNTLIAGTKYGFYMSYDAGTNWTGPCFTNPYTQTITISGGQ
                     HEVRQDNTEILVRKVNTTTTFIYYAIGGAFNNLVGGGTNGANGIYTATLTPATTGCPS
                     PWTLITRGNNGWPAGTGD"
BASE COUNT          273 a          422 c          551 g          341 t
ORIGIN      
        1 gtcaccggtg cctgcgggcc agccgttgtt gcccctggtg atcagggtcc agggtgaggg
       61 gcagcccgtg gtggcggggg tgagcgtggc cgtgtagatg ccgttggcgc cgttggtgcc
      121 gccacccacg aggttgttga aggcgccgcc aatcgcatag tagatgaagg tcgtggtggt
      181 gttgaccttg cgcaccagga tttcggtgtt gtcctgccgc acctcgtgct gcccaccgga
      241 gatcgttatc gtctgcgtgt aggggttggt gaagcagggg ccggtccagt tggtgcccgc
      301 gtcgtacgac atgtagaagc cgtacttggt accggcgatg agcgtgttgc tgttgttagg
      361 gtccaccttg atagcgctca cggcctggta ctggctggag ccgccgatag gctggccggg
      421 accgaagggg ccgttgcgga agggcgggaa gaccgactgg ccgagcacgg tccacgacgc
      481 gcccgcatcg gtgctcttga gtatgccgtt ggagccgcga gccgcaccgg tggcgcggaa
      541 gtcgcccgtg gccgcgtaca cgatgtcggg gttgttcggg tccagcgtga cttcgtcaat
      601 cgtctgggtg ccgatgctgg ggtcgtcggt ggtcgggtac cactgggtat tgagggtgca
      661 gcaggtggtg gtcttccaga cgccgccgtt ggacacaccc aggtacaccg tggaggtaat
      721 ggtggggtga atggcaatcg agttgatgcg cccgctcacc aggccgtact tgaagcagct
      781 cgcgcagggg tcggactggc cgggcctggg gccgaggctg atgagctgtc ccggatcgag
      841 gaccaggggt gagtcggcct gggacctgtc gtagctgatt ttgccggcag gtatgccgga
      901 ctgcatacgg gcgacctgct tctcagcggc gacgagccag ttgttgtcta tctgttgttc
      961 gggcaacccg tagcgggtgc ggaaatactc ttcgcgagag cgcagtatct cgatctcgtc
     1021 ctgagggtcc tggcctgggg cgataatgcc acgcgcctcg ccaatcagcc cattggcggc
     1081 ggtggtggta ccctgcccgg ttgagcttac gttgttgtct tggcggttcg cttgcaaacg
     1141 caacagcgcg gcagtcataa cgacgccgag cagcagccca agcaccagca caatacgcct
     1201 tgtagggacg cggtcctctc ctttagacat tgtcctcttc tcctcttcct tgcgctacct
     1261 cttccttgcg ctagttagat aaatatataa attatagggt aaaatagagg cttttttaca
     1321 taggcttgct ccggggatta caggatacaa agagcgcagg gtcgaggagg cagtaccgcc
     1381 cgtgccaatc gaggactgcg aatgtcgtag agtataccaa ctatgttatg gcaatgacaa
     1441 ggctttcaac cggcaatgac gcaacttgat cgttctcggt ggtgccggga agccctgcgg
     1501 gggtcgccct gtggctaatg aagattacct ggataatcag gtaatggcca ggcacgaatg
     1561 ggtgctgcgt aggggcaggc ccccggg
//
LOCUS       DASRWZ010000830         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_547995, whole genome shotgun sequence.
ACCESSION   DASRWZ010000830 DASRWZ010000000
VERSION     DASRWZ010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547995"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFH60_13210"
     CDS             complement(<1..317)
                     /locus_tag="VFH60_13210"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET6314788.1"
                     /translation="MSKVEHILEVQNRLGEGPRWHPEEQALYWVDIENDYFYRYFPRN
                     GDAEGFSVGMPVGALGFREAGGLVLAVRDGLALWHFGKGRTGRLEFVSDVEADKRDAR
                     FNDG"
     gene            569..1111
                     /locus_tag="VFH60_13215"
     CDS             569..1111
                     /locus_tag="VFH60_13215"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET6314789.1"
                     /translation="MEVIEAIRRKRAVRDYAPGPLPEDVVHQILYAGRRSQSSMNSQP
                     WRFVAVQDPERLAALAQFGDYARHLPSAALAVAILTPDPKAYYWVMFDAGQAAAYMQL
                     AAVELGVGSCIVTLHRPEGSRELLGYPDDLYLTMLVTFGYPADPKALEPASRLGGRQP
                     FDELVHFERYGQHEMQGSGG"
     gene            complement(1309..3177)
                     /gene="treZ"
                     /locus_tag="VFH60_13220"
     CDS             complement(1309..3177)
                     /gene="treZ"
                     /locus_tag="VFH60_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HET6314790.1"
                     /translation="MTWELPIGAQLRENGVRFRVWAEDVRRVEVAILGAEGKEQTAHE
                     LTRDADGYFEGFVAGIGAGTRYMYRLNGDTLRPDPASRYQPEGVHGPSEVVDPAFPWG
                     DGGTWQGLALEETVIYEVHIGTATPDGTFESFIEKLPYLKSLGVNTMEIMPVADFPGE
                     RNWGYDGVDLYAPANAYGGPEGLKRLVDAAHSQGIAVLQDVVYNHLGPDGNYLRDFSR
                     SYFTDAHKTPWGDALNYANPHVRRFFLSNAIYWAREYNMDGLRLDATHAILDDGEPHI
                     LKELPAAVKHSLPPGRHFVICAEDERNEVWLITPPGQGGAGIDAVWADDFHHQVRSAL
                     AGDNEGYYIDFTGSAADLAQTLDRGWFYTGQKSRFSGHARGTDASKHDPARFVYCIQN
                     HDQVGNRPLGDRLTESAGLDAYRAASALLLLSPYTPLLFQGQEWAASTPFLYFTDHNA
                     ELGRLVTEGRRAEFASFKGFHGETVPDPQAASTFMASKLNWHEVEEGEHGQILALYRE
                     LLRLRGSLPAFKERSRANFRSSQVAANAIAMRYLSPTGTDDLLVVVNLRGDLELDFAG
                     AEIMQPPEGQRWQVVLSTEDSRFGGSGSLEELGAGVETSCLKARGPIAVALQAARI"
BASE COUNT          629 a         1052 c         1080 g          646 t
ORIGIN      
        1 ccgtcgttga agcgggcgtc tcttttgtcc gcctcgacat ctgatacgaa ctccaatcgc
       61 cccgttcgcc ccttgccgaa gtgccacaag gccaggccgt cccgcaccgc cagcaccagg
      121 ccgcccgcct cgcgaaagcc caacgccccc acgggcatac ccacgctgaa gccctcggcg
      181 tcaccgttgc gcggaaagta cctgtagaaa taatcgttct cgatgtccac ccagtacagc
      241 gcctgctcct ccgggtgcca cctggggcct tcgcccaacc tgttctgtac ttcaaggatg
      301 tgctcaactt tgctcatggc tgccctctcc cgtgttgaag ttgtgtggct gatgtgccac
      361 gaggtaacga ccatgcaaga agcctgccca gtctaaagtc aagtcattat gctgtaagtg
      421 taaagcacgt aggggagggc tatgccacag tttggacatc acggtgcgct acaccagaca
      481 gaagtgctat attggggcga ctcgacgcaa taatttcctc cgtaagaccg agcggggcat
      541 gggcaatata gaagaaggag acaaggcaat ggaagttata gaggctatcc ggcgcaagcg
      601 ggctgtgaga gattacgcac ctgggccgct accggaggac gtggtccacc agatactata
      661 cgcggggcgc aggtcgcaat cgtcaatgaa ctcgcagccg tggcgcttcg tcgccgtaca
      721 ggacccggag aggttggccg ccctggcgca gttcggggac tacgccaggc acctgccatc
      781 cgccgcgctg gcagtcgcta tcctcacgcc cgacccgaag gcctactact gggtgatgtt
      841 cgacgcgggg caggcagcgg cctacatgca actggcagcg gtggagctag gggtcggctc
      901 gtgcatagtg acgctgcacc gcccggaagg ctcaagggag ctgctgggct accccgacga
      961 cctgtacctc actatgctgg taacgttcgg ctacccggcg gaccccaagg cgctggaacc
     1021 cgcgtccagg ctcggaggcc gccagccctt cgatgagctg gtgcacttcg agcgttacgg
     1081 ccagcacgag atgcagggtt caggtgggta gagtggccct tgtcatcctg agcttagcga
     1141 aggatctaaa gaagccggta gaggatgctg ttgaacatta cggttcatgc gggtgaaggt
     1201 tcagcacagt ccaagaccaa catcttagat ccttcgctac gctcaggatg acaaggtccc
     1261 actacgggtg caagcgttga acgggatggt catggatcga ggcggtgact agatacgcgc
     1321 cgcttgaagg gccaccgcga taggacctct agccttcagg caactcgtct ccacccctgc
     1381 gccaagctct tccaggcttc cagacccacc aaagcgacta tcttcggtag ataggactac
     1441 ctgccagcgt tggccttccg gcggttgcat gatctcagcg cccgcaaagt ccaattctaa
     1501 gtcgccgcgc aggttgacga ccaccagcag gtcgtctgtt ccggtggggc tgagatagcg
     1561 catagcgata gcgttagcgg cgacctggct gctgcggaaa ttggcacggc tgcgctcctt
     1621 gaaggcgggt agactcccgc gcaaccggag cagctcgcgg tagagcgcca gtatttgccc
     1681 gtgctcgccc tcttcgacct cgtgccagtt gagcttggag gccatgaagg tgcttgccgc
     1741 ctgcgggtcg ggcaccgtct cgccgtggaa gcccttgaac gatgcgaact ccgcccgccg
     1801 tccctccgtg actaaccttc ccaattcggc attatggtcg gtgaagtaga ggaacggggt
     1861 gcttgccgcc cactcctgcc cctggaagag cagcggggta tagggcgaga gcagcagaag
     1921 ggccgaggcc gctcggtagg catcgaggcc ggcgctctcg gtcaggcggt cgcccagggg
     1981 gcggttgcct acctggtcgt ggttctggat gcagtagacg aagcgcgccg ggtcgtgctt
     2041 gctcgcgtcg gtgccccgcg cgtgcccgga gaaccgcgac ttctgcccgg tgtagaacca
     2101 gcccctgtca agcgtctgcg ccaggtcggc ggcgctgcct gtgaagtcga tgtagtagcc
     2161 ctcgttgtcg cccgccaggg ccgagcgcac ctggtggtgg aagtcgtcgg cccacacagc
     2221 gtcgataccg gccccgccct gtccaggggg agtgatgagc cacacttcgt tgcgctcgtc
     2281 ctcggcgcat atgacgaagt ggcggccggg tggaaggctg tgtttgaccg cggccggtag
     2341 ctccttgagg atgtggggtt cgccgtcgtc caggatagcg tgagtggcgt cgagacgcag
     2401 gccatccatg ttgtactcac gcgcccagta gatagcgttc gacaggaaga agcggcgcac
     2461 gtgggggttg gcgtagttga gagcgtcgcc ccagggggtc ttgtgggcgt cagtgaaata
     2521 ggagcggctg aaatcgcgca ggtagttgcc gtcggggccg aggtggttgt ataccacgtc
     2581 ctggagcacc gcgataccct gcgagtgagc cgcgtccacc agccgcttca gcccctccgg
     2641 cccaccgtat gcgttggcgg gggcgtacag gtccacccca tcgtagcccc agttgcgctc
     2701 gcccggaaag tcggccacgg gcataatctc catcgtgttg acgcccagcg acttgaggta
     2761 aggcagcttt tcgatgaacg actcgaacgt gccgtcgggc gtggcagtgc cgatgtgcac
     2821 ctcataaatg accgtctctt ccagcgcgag accctgccag gtgccgccat ccccccaggg
     2881 gaaggcgggg tcaactacct cgctcggacc gtgtacgccc tcaggctggt agcggctggc
     2941 ggggtcggga cggagcgtgt cgccgttcag gcggtacatg tagcgggtgc ccgcccctat
     3001 tcccgcgacg aagccctcga agtagccgtc ggcgtcgcgt gtgagttcgt gggcggtctg
     3061 ctccttgccc tcagcaccca gtatcgctac ttccacgcgc cgcacgtctt cagcccacac
     3121 gcggaagcgc acgccgttct cccgcagttg cgcccctatg ggcagttccc aagtcatccc
     3181 agttcctttc tgttagacgt gatgagtgat gcggcttatc gaagctagca cgtgaatgcc
     3241 agggtgcggg aaagcaggac caacgtcccc cttgtcattc tgagcgcagc gaagaatctc
     3301 gtccctcagc acccaagttt cacctttctt tacgccagta tgctgctagg gtgccataga
     3361 aaagggggac atcccggggg aggacgagat tcttcgctgc gctcaga
//
LOCUS       DASRWZ010000831         3329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_205419, whole genome shotgun sequence.
ACCESSION   DASRWZ010000831 DASRWZ010000000
VERSION     DASRWZ010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205419"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VFH60_13225"
     CDS             <1..1360
                     /locus_tag="VFH60_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6314791.1"
                     /translation="QRDRAYGMSRNDVRASEQSADLVRVMEIELLGELEVDADNRLNI
                     AEPNRLPHTGQPVYEIPADVIPQLLGIPTLERIDEGLHLGDLESGGRRVPFLLPTMAI
                     ARHMAVLGKTGVGKSYAVGVLMEELNDKGIPLLSFDVLGDTETTAQELGGRHIVAGSN
                     DFRIPYSILGLSEFLPFIPSLTPDQKDLVASAYGSAYDAASDLLDHGQPLDVPFSTLT
                     NAIQAIGTAISSNATNRAITRAQTAFDRSTLLTDQAVSWPARLRDTPLTNIYVGHLGQ
                     QQRNQVVGAAARILQTLRRRGHIPPFVLVIDEAHLFLPGGQDPPPSTAVLREMIRTAR
                     HDAVGVVLLSQSPSSLDRQILLTANTRLIFSLDPEDLRVVAGQLGDLPEESIQRIPRL
                     ARGRAVVTSAMDIMRHPVIVNIRQRQRTTHVANTPDLREAVREWHEAHPQQDPVHNRN
                     GR"
     gene            1306..>3329
                     /locus_tag="VFH60_13230"
     CDS             1306..>3329
                     /locus_tag="VFH60_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314792.1"
                     /translation="MARGPSTARPGSQPQRAVDPDALATEAAAAIREILQAQGDNIVG
                     LNITELTLHLRTKGVEAQPRFVDGVVRRMRAQDELAQHDDPRRRGGVCYHLPGMGPVQ
                     TSLFDMVATRDEIERQERTESDLWAEDETAREQYTASVMHQIASGHATEERMAELVRD
                     VASVLATENPVALIMEIVRWVVADLNGLAERMRAAPQGRTEEVRGLARELGFRRMKAV
                     RFLQRLWRLDASAEGIPGILDLPTIPKMMDGYQVWANLERVEQQLRKRVIGEHVIEIV
                     SLPENTLQAAVGTDASVGDVMIEHARGSFMPPTPAVLFVAAGALRVRGEAGTAADGEG
                     AGAGAGARYWDYDIDPRELEQYEDLAAAEEGLMISPRLRRDAISDFRHLRSAAMELRQ
                     YVEELRIVQRRSHWYPMGEVVELHHPPEIPLLVRDGRIYPLVHRLDDYDGASAPDDVL
                     YGEVVRREIRTFREVFHSTAGLGRMGAIYGGAVKSPEFSWLAMLTFWYLHARQGHADL
                     ADTFYRPPLSDQAVTHLLFWGLARANRGAALADPRGMFVTFRAVRRYSDIAFPAHPLV
                     IVEERNRVLKIVDEDSEDDWFTFIGQHIDDANESYNLHKRGVPALPGVDEYRPFLDLC
                     RRAGVAMFYAAPARMYRATVEDGSHFLMPRWEVAVDLTGTAAATLQHQLER"
BASE COUNT          640 a         1053 c         1061 g          575 t
ORIGIN      
        1 gcagcgagac cgcgcctacg gcatgagtcg taatgacgtg cgcgcctccg agcaatccgc
       61 cgacctggtg cgcgtaatgg agatcgagct gttgggcgag ctggaagtgg acgccgacaa
      121 ccggctcaac atcgccgaac ccaaccgcct tccccacacc ggccaacccg tatacgagat
      181 acccgccgac gttatccccc aactgctggg tattcccacg ctcgagcgca tagatgaagg
      241 gctgcaccta ggcgatcttg agtctggtgg ccgtcgcgta cccttcctac tgcccaccat
      301 ggctattgcc cgccatatgg ccgtactcgg caagacgggc gtgggcaagt cgtacgcagt
      361 gggcgtgctc atggaagagc tgaacgataa gggtatccct ctactgtcgt ttgacgtact
      421 tggcgacacc gagaccacgg cccaggaact cggcggcagg cacatagtgg ccggctccaa
      481 tgacttcagg ataccttact ctattttagg cctatcggag tttctcccgt ttatccctag
      541 ccttacgccc gaccagaaag acctggtggc gtcggcctat ggtagcgcat acgacgcggc
      601 gagcgacctg ctggatcacg ggcaaccact ggacgtgcct ttctccacgc tcaccaatgc
      661 catacaggct attggcactg cgataagttc aaacgcaaca aaccgggcga taacccgtgc
      721 tcagaccgcc tttgatcgaa gcacccttct caccgaccag gccgtttcct ggcccgcccg
      781 gctgcgggat acgcccctta cgaacatcta cgttggccac ctggggcagc agcagcgcaa
      841 ccaggtggtg ggcgcggcgg cccgcatctt acagacgctc cgcagacgcg gccacatccc
      901 tccctttgtg ctcgttattg atgaggcgca cctattcctt ccgggcgggc aagacccgcc
      961 gccctctacg gcggtgctgc gcgagatgat ccgcacggcc cgccatgacg cggtgggcgt
     1021 ggtgctcctc agccagagtc cctcctctct ggatcgccag atcctgctca ccgccaacac
     1081 ccgcctcata ttcagcctgg atcccgagga cctgagggtg gtggcggggc agctaggcga
     1141 cctgcctgaa gagtccatcc agcgaatccc ccggttggcg cgaggcaggg cagtggtcac
     1201 ctcggctatg gacattatgc gccaccccgt tatcgtaaac atccgccaga ggcagcgcac
     1261 tacgcacgtt gccaacaccc cagatttgcg agaggcggtg agagaatggc acgaggccca
     1321 tccacagcaa gacccggttc acaaccgcaa cgggcggtag accccgacgc cctcgctacc
     1381 gaggctgcgg cggccatcag ggaaatactg caagcccagg gcgataacat agtcggcctg
     1441 aacataaccg agctgaccct gcatttacgc acgaaaggtg tagaggcgca gccgcgcttc
     1501 gtggacggcg tagttcgccg catgcgcgcc caagacgagc ttgcccaaca tgacgaccct
     1561 cggcggcgtg gcggcgtgtg ctaccacctg cccggcatgg gcccggtaca aaccagcctg
     1621 ttcgacatgg tggccacccg agacgaaatc gagcggcagg agcgaacgga gtccgatctt
     1681 tgggcagagg acgaaacggc ccgcgagcag tacacggcat cggtgatgca ccagatagcg
     1741 tcggggcacg ctaccgagga gagaatggcc gagctggtgc gcgacgttgc ctcggtgctg
     1801 gctacagaga accccgttgc cctcatcatg gagatcgtgc gctgggtggt agccgacctc
     1861 aacgggctgg ccgagcggat gcgggcagcg ccccagggcc gcacggaaga ggtgcgcggg
     1921 ctggcgcgtg agctgggctt tcgtcgtatg aaagccgtgc gcttcctaca gcgcctatgg
     1981 cggctcgacg cctcggcaga gggcatcccc ggcatcctgg acctgcctac catacctaaa
     2041 atgatggacg gctatcaggt ttgggctaac ctggagcgtg ttgagcagca gttgaggaag
     2101 cgggtgatcg gcgagcacgt gatcgagata gtatccctgc ccgaaaatac cctgcaagcg
     2161 gcggtgggga cggacgcttc cgtaggcgac gtgatgatag agcacgccag gggcagcttc
     2221 atgccgccca ctcccgccgt gctatttgta gcggcggggg cattacgagt tcgcggggaa
     2281 gccggtacgg ctgcggacgg cgaaggggcc ggggccgggg ccggagcgcg ttattgggac
     2341 tacgatattg accctcggga gctagagcag tacgaggacc tggcggcagc cgaagaggga
     2401 ctgatgatat cgcccaggct gcgccgcgac gccatatccg actttcgcca cctgcgctcg
     2461 gcggctatgg agcttcggca gtacgttgag gagcttcgca tcgttcagcg ccgctcgcac
     2521 tggtatccga tgggcgaggt ggtagagttg caccacccac cggagatacc tctcttggtg
     2581 cgcgacgggc gcatctaccc cctggtgcac cggcttgacg actacgacgg ggcaagcgcg
     2641 cccgacgacg tgctgtacgg cgaggtggtg aggcgagaga tacgcacctt ccgcgaggtg
     2701 ttccatagta cggctgggct gggtaggatg ggggcgatct acggcggcgc ggtgaagtcc
     2761 ccggagtttt cctggcttgc catgctcacg ttctggtacc tgcacgccag gcaaggccac
     2821 gccgaccttg ccgacacgtt ctaccgcccg cccctcagcg accaggcggt tacccaccta
     2881 ttgttctggg ggctggcgcg ggcaaaccga ggggccgcct tggccgaccc acgtggtatg
     2941 ttcgttacat tccgtgcggt aagacgctac tccgacatcg cgtttccggc gcacccactc
     3001 gtgatcgtag aggagcgcaa tcgcgtgctc aagatagtag acgaggactc cgaggacgac
     3061 tggttcacgt tcatcgggca gcacatagat gacgcgaacg agagttacaa cctgcataaa
     3121 cggggcgttc ctgcgctgcc gggcgtggac gagtacaggc cattcctgga cctgtgtagg
     3181 cgggccgggg tagccatgtt ctatgcggcc cctgccagga tgtatagggc aaccgtagag
     3241 gacggctcgc acttcctgat gccacgctgg gaggtggccg ttgacctcac cggcaccgcc
     3301 gcggccaccc tgcagcacca gctcgagcg
//
LOCUS       DASRWZ010000832         4143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719 k141_68081,
            whole genome shotgun sequence.
ACCESSION   DASRWZ010000832 DASRWZ010000000
VERSION     DASRWZ010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68081"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1785
                     /locus_tag="VFH60_13235"
     CDS             109..1785
                     /locus_tag="VFH60_13235"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET6314793.1"
                     /translation="MRRLANPYVKRGKSRLPHPAFVVMGALLLAWVVFSPAAFSLAQQ
                     GQQDFANAAFRAMWDRTDGPVAAGATLRPWIWGPAPGRTLPEPFAGLPGNNHLVQYFD
                     KGRMEINDPNGDPRDPFYVTNGLLAVELISGQMQTGRDTYENRGPATINLGADADDAT
                     APTYQSFNGVSNIPGAPNERRKTDQTGQVVRTAIDRQGLTQPWPATHPDYGVRIAYFE
                     PTTGHNVPNVFWDFLNAEADIFVEGDLAPGRLFEPWFSVTGLPISEAYWSYVKVEGRY
                     MDVLTQAFERRVLTFLPQLPTPFKVQMGNIGQHYYEWRYLRNANPGTPAPQRPVPTPT
                     GASRPAVANVTIDEIHPRTSLVDLNSNYCVITNRGQAAVNFTGWWLDSPKWTVVDRYY
                     FPAGFTLQPGASVNVNAGTGNNSATDLYMFRSTAMWDGQPYDLAVLYDNLGREVNRLF
                     PASDVGQPPAPTVPGGSPSATPRPGGTTIATASVPTRGSPTLTPTTGVPTAQSSIVAT
                     VGTRTATPVASGTATATVTGTPPTATATVTGTPPTATITTTPTRTPTRAP"
     gene            complement(1968..3962)
                     /locus_tag="VFH60_13240"
     CDS             complement(1968..3962)
                     /locus_tag="VFH60_13240"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HET6314794.1"
                     /translation="MAHTIVILVDCLRPPDQEKGYFAPGSEGLFLLRLGVLLAGSGEA
                     RGRVLLCRFLTVPEGEAVSSYSVAAQAMRQKLEGEALAALAQIEAELSEESRSPSNAH
                     IAPVVRVALENELAQDARRFLRGEDGALALLPFRRLTKDTCPWIRKALRNPLPCDVAW
                     ARPPAGYEQEREPDMSRGKPFHKGMKVLLPARGGPQAVLAMEIARDLTIKLDARATVL
                     HVLKSMPELARATEEAPFAEFMDEMERAERRNNPLARRFERAFVVGADPQKNIIGAAE
                     GHDMLIMGTGGRAAADIGSFTEGVAKGVDATVVALKTKTPVIPEIQAARARARTRMHA
                     IAPEALSLLVDKWFAENTFDAGEFSDLRRMVELKRQQGVTISLGLPALNEEATIGDVI
                     TVLKRSLMEEVPLVDEIVLIDSNSTDRTREIAAELGVPVHIHQEVLPETGVYSGKGEA
                     LWKSLHVLKGDIVAWVDTDVVNMQPQFVYGLVGPLLREPRIGYVKGYYHRPIRTERAM
                     QQEGGGRVTELTARPLINLFFPLLSGLVQPLAGEYAGRRSVLEKIPFFSGYGVEVGML
                     IDILEGFGLDAIGQVNLESRVHRNRPLSDLSLTAFAIVQVIMGRVERRQRTTLLEEVN
                     TTMKLIRFEKDHLSLEVRNVRDVERPPMLEVAKYWNKGKG"
BASE COUNT          715 a         1393 c         1297 g          738 t
ORIGIN      
        1 gcagctcgtg ccgttcttct tccctgctac ttcctactga ggcccacgcc gggcacacgg
       61 agcagacctt tttatgccta ccgaccctcg tccaggagac gattcgccgt gcggcggctt
      121 gccaatccct acgtgaaacg gggcaagtcg cgcctgccgc acccggcttt tgtcgtcatg
      181 ggcgcgctcc tgctggcttg ggtagtgttc tcgcccgccg ctttctcgct cgcgcagcag
      241 gggcagcagg acttcgccaa cgccgctttc agggctatgt gggaccgcac cgatgggccg
      301 gtggccgccg gggctaccct aaggccgtgg atatggggtc ccgcgccggg gcgcacactg
      361 ccggagccgt tcgcggggct gccgggcaac aaccacctgg tgcagtactt cgacaagggc
      421 cgcatggaga tcaacgaccc caacggcgac ccccgcgacc ccttctacgt gaccaacggc
      481 ctgctcgcgg tggaactcat ctccggccag atgcagaccg gacgcgatac gtacgagaac
      541 cgaggccccg ctaccataaa cctgggggct gacgcggacg acgccaccgc ccccacttac
      601 cagtcgttca acggcgtgtc gaacataccc ggcgcaccca acgagcggcg caagacggac
      661 cagaccggcc aggtggtgcg cacggcgatt gaccgtcagg gcctcaccca gccgtggccc
      721 gctacccacc ccgactacgg cgtgcggatt gcttacttcg agcctaccac cgggcataac
      781 gtccctaacg tgttctggga cttcctgaac gcggaggccg acatattcgt ggagggcgac
      841 ctcgcgccgg gcaggctgtt cgagccgtgg tttagcgtca cgggcctgcc catctccgag
      901 gcgtactggt cgtacgtgaa ggtcgagggg cgctacatgg acgtgctgac ccaggcgttc
      961 gagcggcgcg tgctcacgtt cctgccccag ctgcccacgc ccttcaaggt gcagatgggc
     1021 aacataggcc agcactacta cgagtggcgc tacctccgca acgccaaccc cggcacgcct
     1081 gcgccgcagc gccccgtgcc cacgcctacg ggtgcctcgc ggcccgccgt ggctaacgtc
     1141 acgatagacg aaatccaccc ccgcacctca ctggtggatt tgaacagcaa ctactgcgtc
     1201 atcaccaatc gcgggcaggc ggcggtcaat tttacgggtt ggtggctcga tagccccaag
     1261 tggaccgttg tggaccgcta ttacttcccg gcgggcttca cgttgcagcc tggggcttcg
     1321 gtgaatgtga acgccgggac gggtaacaac tcggccacgg acctgtacat gttccgcagc
     1381 acggcgatgt gggacgggca gccgtacgac ctcgcggtgc tctacgacaa cctgggcaga
     1441 gaggtgaaca ggctcttccc cgcgtccgac gtgggccaac ctcccgcgcc tacagtgccg
     1501 ggcgggtctc cgagcgccac ccctcgcccc ggcggcacca cgattgccac cgcctcggtg
     1561 cccaccaggg gttcgcccac gctcacgccc acgaccggcg tgcccacggc ccagagcagc
     1621 atcgtagcca cggtaggcac ccgcaccgcc acgccggtcg cgagcggcac cgctacggcc
     1681 acggtgacgg gcaccccacc cactgccaca gccaccgtga cgggcacccc acctaccgct
     1741 accatcacca ccacgcccac ccgcaccccg actagagcgc cgtagccctg ggacgttgaa
     1801 agtagacgaa aacgcaaaga cgcaaagacg caaagaacgc gcagagatta aggattgttg
     1861 ttttgagtat atagaggacc agggtgcatg ataccgcgct ctagtccgcg ttgtactata
     1921 taaacctttc ttctctttgc gcgttctttg cgtctttgcg ttttcgtcta tcctttaccc
     1981 ttgttccagt acttagctac ttcgagcatg ggggggcgtt ctacgtcgcg gacgttgcgg
     2041 acttcgaggg agaggtggtc tttctcgaag cggatgagtt tcatggtggt gttgacctct
     2101 tcgaggaggg tggtgcgctg gcggcgctcc actcggccca tgatgacctg gacgatggcg
     2161 aaggcggtga gcgacaggtc ggacaggggc cggttgcggt gcacgcggct ttcgaggttc
     2221 acctgcccga tagcgtccag cccgaagcct tccaggatgt ctatcagcat gccgacttcc
     2281 acgccgtaac ctgagaaaaa gggtattttc tccagcacgc tgcggcggcc cgcgtattcg
     2341 cctgccaggg gctgcacaag gccggacagc agcgggaaga acaggttgat gagcggcctg
     2401 gcggtaagct cggtcaccct gccgccgccc tcctgctgca tggcccgctc ggtgcggatg
     2461 gggcggtggt agtagccctt tacgtagccg atgcgcggct cccgcaacag cggccccacc
     2521 agcccgtaca cgaactgggg ctgcatgttc accacgtcgg tgtccaccca ggccacgatg
     2581 tcgcccttga gcacgtgcag cgacttccag agggcctccc ccttgccgct gtaaacgccc
     2641 gtttcgggca gcacctcctg gtggatgtgg accggcacgc caagctcggc ggcgatttcg
     2701 cgggtgcggt cggtcgaatt ggagtctatc agcacgatct catcgacgag gggcacctct
     2761 tccatcagcg accgcttgag caccgtaatc acgtcgccta tcgtggcttc ctcgttcagg
     2821 gcgggcaggc ccagcgagat tgttacccct tgctggcgct tcaactcgac catgcggcgc
     2881 aggtcggaga actcgcccgc gtcgaaagtg ttctccgcga accacttgtc cacaaggagg
     2941 gagagggctt cgggggcgat ggcatgcatg cgagtgcggg cgcgggcgcg ggcggcctgt
     3001 atctccggga tgacaggggt cttggtcttt agggccacca ccgtcgcgtc cacgcccttc
     3061 gccacgccct ccgtaaagct gccgatatcc gccgctgccc ggcccccggt gcccatgatg
     3121 agcatgtcgt ggccctcggc cgcgcctatg atgtttttct gggggtccgc gcccaccacg
     3181 aaggcgcgct cgaagcgcct ggcgaggggg ttgttgcgcc gctcggcccg ctccatctcg
     3241 tccatgaact cggcgaaagg ggcctcctcg gtggctcgcg caagctccgg catcgatttc
     3301 aagacgtgca gcaccgtggc ccgcgcgtca agcttgatcg tgagatctct ggctatctcc
     3361 atagcgagca cggcttgggg gccgccgcgg gcgggaagca gcaccttcat gcctttgtgg
     3421 aacggcttgc cccggctcat gtccggctcg cgttcttgct cgtacccggc gggagggcgc
     3481 gcccaggcca cgtcgcaggg gagggggttg cgcagcgcct tgcgaatcca ggggcaggtg
     3541 tccttcgtca ggcggcggaa gggcagcagc gcgagcgcgc cgtcttcgcc acgcaagaag
     3601 cggcgggcgt cctgggccag ctcattttcg agggccacac gcaccacggg cgcgatgtgg
     3661 gcgttagagg gggagcggct ttcctcgctc agctcggcct cgatctgggc cagcgcggcc
     3721 aacgcctcgc cttcgagctt ctgcctcatc gcctgggccg cgaccgagta gctgctcacc
     3781 gcttcgcctt cgggcaccgt gaggaacctg cacagcagca cgcgcccgcg agcctccccg
     3841 ctcccggcca gcagcacccc cagcctcaac aggaacagcc cctccgagcc tggagcgaag
     3901 tagccctttt cctggtccgg cggcctcaga cagtccacca ggatgacgat tgtgtgcgcc
     3961 atgagagcgg tccccttcgg gaattacgaa ttacgaatta tgagaaagac ggaaacgcaa
     4021 agacgcaaag gtcgcaaaga acgcgcaaag gttaggattg tttggtgttg gcctgtagtt
     4081 agcaactcgt aattcgtaat tcgtaattcg taattcgtaa ttaccgtcag gccagcgtgg
     4141 cga
//
LOCUS       DASRWZ010000833        11267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_821889, whole genome shotgun sequence.
ACCESSION   DASRWZ010000833 DASRWZ010000000
VERSION     DASRWZ010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11267
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821889"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            782..1579
                     /locus_tag="VFH60_13245"
     CDS             782..1579
                     /locus_tag="VFH60_13245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6314795.1"
                     /translation="MKNTRRPHNHPRRASPATSWDAVAHWYDGWVGEDGSEHHREVAV
                     PTLLSLLDPRPGEHILDIGCGQGVLAPHIAGAGARYTGVDASPRLLDIARRRHGKEGR
                     FFLGDACGLAAVQGLREGSYDGVTFLLSIQDMDPLEKVLASAAWALTPGGRLVALLTH
                     PAFRVPRQSGWGWDEGRRLQYRRVDRYLTPLPVPMKSVDGSRPTRSFHRPVSAYVNGL
                     AACGLVVDNMLEVPAHRVKSPRAGDERAAELARREIPLFLALRARKG"
     gene            complement(1631..2239)
                     /locus_tag="VFH60_13250"
     CDS             complement(1631..2239)
                     /locus_tag="VFH60_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314796.1"
                     /translation="MLKLRRIWPFGLVLLLASCVPVGGGAAVPVPQDFSPTPPQVALA
                     TPTSTAPVTPTEAVVAKQATAPDRETANCPVTQPPELPFTPPPPYPPTAPWAGSFWYG
                     TDLLWTQVPGDGTWSGPTQKVFWWREGYSWKEDPTPQLTVTGRRLDAPAPPLDASTAT
                     NAFDEDIQSAMLVGVDFPTSGCWEITGKVADAELSFVVWIGP"
     gene            complement(2336..2824)
                     /locus_tag="VFH60_13255"
     CDS             complement(2336..2824)
                     /locus_tag="VFH60_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014282579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6314797.1"
                     /translation="MEAIIFTGLQASGKSSFYRQRFFDTHVRINLDMLRTRNRERVLL
                     QACLEMKQPFVVDNTNPTPDDRARYIGPARQAGFRVVAYYFQPDLQGSRARNDLRTGK
                     ARVPVGAISGTAGRVQSPPWEEGFEAIYDVWVADGGFRVVERLREAGHQENPGYMKGF
                     TG"
     gene            complement(2856..3815)
                     /locus_tag="VFH60_13260"
     CDS             complement(2856..3815)
                     /locus_tag="VFH60_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6314798.1"
                     /translation="MATTTGTETTITTAKPTVKRSREAVRRRLAVLAITLVAFVLAAY
                     IGVSGIVADKLSHPTRKAVLRTPEELNMQFEDVLFNSTVDNIPLKGWYIDSPGDKVIL
                     VMHGRNSTRDGGDILDIIKGLYDRGYDAFAFDFRAHGLSGGERYSLGLLETRDVAGAL
                     LYLKERGVTEVGGLGFSMGAATELLAAPDHPEMKTLVSDSAFANLPNLLDKELPKASG
                     LPSLFNPGIIMMGKLFYGIDLAATKPVESLPRLRDRPLFLIHGTEDATVPVSHAYELQ
                     KAAEGNPGLQSWVVEGAGHTGSYDKAPQEYMQRLIAFYDKYLP"
     gene            complement(3837..4913)
                     /locus_tag="VFH60_13265"
     CDS             complement(3837..4913)
                     /locus_tag="VFH60_13265"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET6314799.1"
                     /translation="MATTDKTNIGVDSQADASGARLRVATLEEVGLQSPEDVLLRVAG
                     LRMHFPIMRGVLFQRQVGAVRAVDGLDFFVKKGETLGLVGESGCGKSTAGRAILQLYK
                     PTAGKVVFKGENIVGLPGEQMRHLRRRMQMIFQDPYASLNPRQTVSDIIGEPLKVHGL
                     AEGKGRQERVLDLLRRVGLNPYHANRYPHEFSGGQRQRIGIARALAVQPEFIVADEPI
                     SALDVSIRAQIINLMEDLQAEFGLTYLFIAHDLSVVRHISDRVAVMYLGKIVELADRN
                     ELYANPMHPYTEALLSAVPIPDPMAEMQRQRIILTGDVPSPINPPRGCHFHTRCPLAI
                     AACKEIDPPLQEKSPGHWAACIRV"
     gene            complement(5125..6147)
                     /locus_tag="VFH60_13270"
     CDS             complement(5125..6147)
                     /locus_tag="VFH60_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6314800.1"
                     /translation="MPHLLEVNNLQTYFYTAEGVVKAVDGVSFTLDERETLGIVGESG
                     SGKSVTAMSIMRLIASPPGKVVGGEILYRGENLLNYSREDMRHIRGKEISMIFQDPMT
                     SLNPVLKVSTQIGESLQLHMDMTQAQARTRTVELLKLVGIPSAQERVDNYPHQFSGGM
                     RQRVMIAMALSCNPKLLIADEPTTALDVTIQAQILDLIRRLKSEFNTAVIFITHDLGV
                     VAGLCDRVIVMYGGQVMEQGTTMQMYKDPRHPYTLGLLKSVPRLDEERKERLEPIPGL
                     PPDLIRMAPGCPFYARCTYREPRNELERPPLRTVDEGHQVACWIDVRTTETHDIEDET
                     AVKAGS"
     gene            complement(6248..7198)
                     /locus_tag="VFH60_13275"
     CDS             complement(6248..7198)
                     /locus_tag="VFH60_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6314801.1"
                     /translation="MLLRQKAVEPGILRPTRSASMWRNATRHLLRNRSGQVGLVIIGI
                     LLFVAIFAPIIATHDPLTFTDPNNRVRITPCIHLFGCPSNEPQHIFGLDGNGRDLFSR
                     VVHATRVSLFIGVATVTFAIIVGTLIGALSGYIGGWLDNILMRLMDVLLAFPSLLLAI
                     ALVAVLRPLIVQGLSPLLPALFAIGFVAIPVYARIVRASVLSIRELDYVTADKALGVP
                     AGRTLFRTILPNALTPLIVQGTLGIASGILDAAALGFLGLGQQPPHPEWGTMLGVERN
                     SVFNAPHLLIFPGLAIMITVLAFNLLGDGLRDALDPRLNR"
     gene            complement(7209..8285)
                     /locus_tag="VFH60_13280"
     CDS             complement(7209..8285)
                     /locus_tag="VFH60_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6314802.1"
                     /translation="MARFLLQRLLLIVPVILGIIFVTFILVRSIPGDPCIAMLGERAS
                     AAACADFRVRFGLNDNILVQFGRYTTGLATADLGNSIKFGRPVGDILLERLPMTLELA
                     IGSMLFAIAVGVPLGIISAYRHKSALDAGAMIIANLGVSMPIFWLGLLLAGFFGVVLR
                     DTPFQLPTGSRLTPGLALPPLDKAWNMGELEGVARGIFLFVSNMFTLNATLQGKWNVL
                     GDALEHLILPSVALGTIPMAIIARLTRSNLLDVLGADYIRTARSKGLGERFVLFRHAM
                     RNALLPLVTVIGLQFGALLGGAVLTETVFGLPGVGKFLVDSIFARDYPVVQGFTIIVA
                     MLFVLMNLLVDISYAYIDPRIRLS"
     gene            complement(8504..10303)
                     /locus_tag="VFH60_13285"
     CDS             complement(8504..10303)
                     /locus_tag="VFH60_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6314803.1"
                     /translation="MFSMKKSAGLLAASLILLPMLAACGPEAAPTPTTVQQATATAPP
                     AAQATNTTAAAPTDTVAAPEETPTEEAVASPTGSSASITATDDLYAVSDCSYGGIMKS
                     IEAVDETTVKFTLCASDPAFPSKIAFSSLGIQSAAHLEETGGAPLDEPLGSGPYMVQE
                     WVRGDHLTMVPNPNYDGEAPAFKTLIFRWNESATARLSSLRAGEADGIDNPDPNDFAT
                     IEGDANLKLFPRVALNVFYVGMNNTKPPLDNEKVRQAIAMALNRQAIVDKFYPPGSAV
                     AQYFTPCAIPGGCEGDPWYEYNVDQAKALLAEAGFPNGFNIRLAYRNVVRGYLPSPDK
                     VAEELQAQLAPLGITATIDQQESATFLDNASAGNLEMFLLGWGADYPDATNFLDSHFG
                     AGANDSFGTKWPDVTSKLAQAASLSDQEQRNALYGDANTAIKQHVPMIPIAHGGSAVV
                     YRADVVGAHSSPLGNESFRVMSAPGRDTFVWIQNGEPPSLYCADETDGEALRACEQIF
                     EPLLGYATGSTEVELGLAASYESNTDLTEWTIKLRPNAKFSDGTAVTARDVVATYAVQ
                     WDAKHPLHKGRAGNFDYWTYLFTAFLNPPPPAE"
     gene            10598..11116
                     /locus_tag="VFH60_13290"
     CDS             10598..11116
                     /locus_tag="VFH60_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6314804.1"
                     /translation="MLLVALKGMPGSGKSTLGRGISRRLGWPIIDKDDVKDLIDGHCD
                     DSATLAYSVMFNIARRQLQQGLNVICDSPLTYAGLYAQARQVASEAGAKLVVLECMCS
                     DEAEWRRRVDARGELGLPSHHMSSWDKLQAYRGMVEGKVDYPVADERLVLDTCGAVEE
                     LVEEAVGWLREH"
BASE COUNT         2152 a         3370 c         3653 g         2092 t
ORIGIN      
        1 tagttgttag tggttagtgg ttagtggtta gtggttagag gctcttcact gaccactaac
       61 cactaacaac caacaactaa cgacttaaac aaaaaactcc cgtccccaaa tcaatgaagg
      121 gacgagagcc aggctctgcg cggtgccacc ctacttcggc acgtcgtgtg ccgcactcaa
      181 caggtaccta gtcgctggcg tggctacgat gccatacgtc tgccgatacc cggcttcctg
      241 ataacggtga agtctccggc gcagtctact cgacgagctt tcggtgcgca actcccagat
      301 ccattcacca cacgcgctgg tgccggttca cacctactcc ggctctctgt accccgctct
      361 tgcagctact cgttctgttc gcggttgttg tcctatgcga ttgagatgac tataacacag
      421 gcatgtaagc caagtcaaga gggtaagcgg catttggcga cataatgcca ggagtaaagc
      481 taatctacat ctccagccgg attgaccgcc cccgccccac cgggttatac tcgcgcagtg
      541 gccgagttga gcgccacacc cacacggaga aggttgtgac agtgatacgc atacgcacaa
      601 actagcggca aagcactagc cgctacataa gctttcgtgc tcccccggcc tgcattcagg
      661 ctgaccgggc gcgactgcgc tttgccgcac cgacaccatg acacgcagca gcgaacctgc
      721 tggtgtgttg tggttttttg tttgtccggc agccgcagtc acgcaaggaa ggagctatac
      781 cttgaagaac acacgacgac cccacaacca cccacgcagg gcctcccccg ctacaagctg
      841 ggacgcagtg gcgcactggt acgacggttg ggttggagag gacggaagcg agcaccaccg
      901 cgaggtagcg gtacccaccc tgctgtccct gctcgaccca cggcccggcg agcatatcct
      961 cgatataggc tgcggacagg gcgtgctggc accgcacata gccggggcgg gcgctcggta
     1021 tacaggcgtg gacgcaagcc ctcgtctgct cgacatcgcc cggcgcaggc acggcaaaga
     1081 aggccggttc ttcctgggcg acgcctgcgg cctggcagcg gtgcaagggt tgcgagaggg
     1141 cagttacgac ggcgtgacgt tccttctcag catacaggac atggacccac tggagaaggt
     1201 gctggcgtcg gcggcctggg cgctcacacc tgggggcaga ctggtggccc tgctcaccca
     1261 ccctgcgttc cgggtgccgc gccagagcgg ctggggctgg gacgagggcc gcaggctgca
     1321 gtaccgccgg gtggaccgct acctgacgcc cctccccgtg ccgatgaaga gcgtggacgg
     1381 gtcgcgcccc acccgcagct tccaccgccc ggtgagcgcc tatgtgaacg gcctggcggc
     1441 ctgcggcctg gtggtggaca acatgctgga ggtgcccgcg caccgggtga agtcgccacg
     1501 cgcgggcgac gagcgggcgg cggagttggc gaggcgggag ataccgctct ttctggcgct
     1561 cagggcgagg aagggttagc gccttgcaag acccgcgcat caatttagcg cagtgagtct
     1621 gctcgcgttc ctaaggtccg atccagacga cgaagctcag ttccgcgtct gcaactttgc
     1681 ccgtgatttc ccagcagccg gacgtgggaa agtccactcc taccagcatg gcggattgaa
     1741 tgtcctcgtc aaaggcgttt gtagccgtgg aggcatcgag gggcggggcg ggcgcatcta
     1801 gccgcctgcc ggtcacggtg agttgaggcg tagggtcctc cttccaggag tagccttctc
     1861 gccaccagaa caccttttgt gtgggtccag accaggtgcc atctcccggc acttgcgtcc
     1921 agaggaggtc cgtgccgtac cagaagctac cggcccaggg tgcggtgggc gggtaaggcg
     1981 gtgggggcgt gaagggcaac tccgggggct gtgtcacagg gcagttggca gtctcgcggt
     2041 cgggcgctgt ggcctgtttt gccacgaccg cctccgtggg tgtcacaggc gcggtgctag
     2101 taggagttgc cagggcgacc tgcggaggtg ttggcgaaaa gtcttgcggc acggggacgg
     2161 cagcgcctcc gccgaccgga acgcaggaag caagcagcag gacaagcccg aacggccaga
     2221 tacgacgcaa tttcagcata aatgcctccc caacaggtac aggatgttgt tgctctagat
     2281 tacaccgcaa caagataccc ggttccatcg agacgcattc aaagaggtcg tacgattacc
     2341 ctgtaaaacc cttcatatat cctggattct cctggtgacc agcttccctc agcctctcca
     2401 ccaccctgaa gcctccatcc gccacccaca cgtcgtaaat cgcctcgaag ccctcctccc
     2461 aggggggaga ctgcacccgc cccgccgtgc cggagatggc acccaccggg acgcgggcct
     2521 tgccggttcg gaggtcgttg cgggcgcggg agccttgcaa gtcgggctgg aagtaatagg
     2581 ccaccacgcg gaagccggcc tggcgggcgg gtccgatgta ccgggcgcgg tcgtcggggg
     2641 tggggttcgt gttgtccact acgaagggct gcttcatctc caggcaggct tgcagcagca
     2701 cgcgctcccg gttgcgcgtc cgcagcatgt cgaggttgat gcgcacgtgc gtgtcgaaga
     2761 atcgctgcct gtagaaggac gattttccgg aggcttgcag gccggtgaat atgatggctt
     2821 ccatcagtaa ggaaatccga aattcgaaat tcgaattacg gcaggtattt gtcgtagaag
     2881 gcgataaggc gctgcatgta ttcctgtggg gccttgtcgt agctgccggt atggcccgcg
     2941 ccctctacca cccaggattg caagccgggg ttgccctccg cggccttctg tagctcgtag
     3001 gcgtggctga cgggcaccgt agcgtcctcc gtgccgtgta tgaggaagag ggggcggtcg
     3061 cgcaggcgtg ggagggactc cactggcttg gtggcagcca ggtcgatgcc gtagaacagc
     3121 ttacccatca tgatgatgcc cggattgaag aggctcggta ggccgctggc tttcggcagt
     3181 tccttgtcca agaggttggg caggtttgcg aaggccgagt ctgaaacgag ggtcttcatc
     3241 tccgggtggt cgggtgccgc cagtagctca gtggccgcgc ccatagagaa ccccaggccg
     3301 cctacctcgg tcactccccg ctccttcagg tacagcaacg cccccgccac gtcccgcgtc
     3361 tccagcaagc cgagggaata ccgctcgccg ccggatagcc cgtgggcgcg gaagtcgaac
     3421 gcgaacgcgt cgtagccccg gtcgtacagc cccttgatta tgtcgagtat atcgccgccg
     3481 tcgcgcgtgc tgttgcggcc gtgcatcacc aggatgacct tgtcgccggg agagtcgatg
     3541 taccagccct tgagcgggat gttgtccacg gtgctgttga acagcacgtc ctcgaactgc
     3601 atgttcaact cttcgggcgt gcgtagcacc gccttgcggg tggggtggga gagtttgtcg
     3661 gctacgatgc ctgatacccc tatgtaggcg gccaggacaa acgccacgag cgtaatggca
     3721 agcaccgcca gcctgcgccg caccgcctct ctactgcgct ttacagtggg tttcgctgtt
     3781 gttatcgtcg tttcggtccc ggtggttgtt gccattgtct cctctagtct aaaatgtcac
     3841 accctgatgc aggccgccca atgccccggc gacttctcct gcaacggcgg gtctatctct
     3901 ttgcaggcgg cgatagcgag ggggcagcgg gtgtggaagt ggcagcccct cggcggattg
     3961 atgggtgagg gcacgtcgcc ggtgaggatg atgcgctggc gctgcatctc ggccattggg
     4021 tcagggatgg gcaccgcgga cagcaacgct tcggtgtagg ggtgcatcgg gttggcgtat
     4081 agctcgttgc ggtcggcaag ctccacaatc ttgcccaggt acatcacggc cacgcggtcg
     4141 gagatgtggc gcaccacgct caggtcgtgg gcgatgaaca ggtaggtcag gccgaactcc
     4201 gcctgcaagt cctccatcag gttgataatc tgggcgcgga tcgacacgtc gagggcggaa
     4261 attggctcat cggccacgat gaactcgggc tgcaccgcca gcgcgcgggc gatgccgata
     4321 cgctgcctct ggccgccgga gaactcgtgc gggtagcggt tggcgtggta ggggttgagg
     4381 cccacgcggc gcagcagatc aagcacccgc tcctggcggc ccttaccctc cgcgaggccg
     4441 tgcaccttca gcggctcccc gatgatatcg gacaccgttt gccgggggtt gagggaggcg
     4501 taggggtcct ggaagatcat ttgcatccgg cggcgcaggt ggcgcatctg ctcgcccggc
     4561 aggcccacga tgttctcgcc cttgaagacc accttgcccg cggtaggctt gtaaagctgc
     4621 aatatggcgc gccccgccgt gctcttgccg cagcccgact cgcccacgag gcccagcgtc
     4681 tcgcccttct tcacgaagaa gtccagccca tccaccgccc gcaccgcgcc gacctgccgc
     4741 tggaacagca ccccccgcat gatgggaaag tgcatccgca gccccgccac ccgcagcagc
     4801 acgtcctccg gcgactggag accgacttcc tccagcgtcg cgacccgcag ccgcgccccc
     4861 gaagcatcgg cctgcgaatc gacccctatg ttagtcttgt ccgtagttgc catatcgacc
     4921 cttcaagata caagtttacg aaaacgcaaa gacgcaaagg acgcaaagaa cgcaaagaac
     4981 gcgcagagat aaagattgtt gttcaaactt tagttacttc cctcaccaag ttgaacgcta
     5041 tggcaacgtg ctacttccga caagaacaaa cctttctact ctttgcgcgt cctttgcgtc
     5101 tttgcgtctt tgcgttttcg tctcctaaga cccggctttg acggctgttt cgtcttctat
     5161 gtcatgtgtt tcggtggtgc gaacgtctat ccagcaggct acctggtgtc cctcgtctac
     5221 cgtgcgcagg ggcgggcgct ctagctcgtt gcgcggctcg cggtaggtgc agcgggcgta
     5281 gaaggggcag ccgggggcca tgcgaatcag gtcggggggc aggccgggga taggctccag
     5341 ccgctccttg cgctcctcgt ccagccgggg caccgacttc agcaggccca gcgtgtaggg
     5401 gtggcgtggg tccttgtaca tctgcatggt cgtgccctgc tccatcacct ggccgccgta
     5461 catcactatc acccggtcgc acaggcccgc caccacgccg aggtcgtgcg tgatgaagat
     5521 cacggccgtg ttgaactcgc tcttgagacg gcggatcagg tcgagtatct gcgcctggat
     5581 ggtcacgtcc agcgcggtgg taggctcgtc ggcaatcagc agcttggggt tgcacgagag
     5641 ggccatcgct atcatcacgc gctggcgcat gccgccggag aactggtggg ggtagttgtc
     5701 cacgcgctcc tgggcggacg gtatgcccac cagcttgagc agctcgaccg tgcgggtgcg
     5761 ggcctgggcc tgcgtcatgt ccatgtgaag ctggagcgac tcgcctatct gcgtgctcac
     5821 cttcagcaca gggttcaggg aggtcatggg gtcctggaag atcatcgaga tctccttgcc
     5881 ccgtatgtgg cgcatgtcct cccggctgta gttgagcagg ttctccccgc gatacaggat
     5941 ttcgcccccc accaccttac cgggcggtga ggctatcagg cgcatgatcg acatggcggt
     6001 tacgctcttg ccggagcccg actcgcccac gatgccaagc gtctcgcgct cgtccagcgt
     6061 gaacgacacg ccgtccaccg ccttcaccac gccctcggcg gtgtagaagt acgtctgcag
     6121 gttgttcact tcgagtagat gtggcattag agacctttct ctcgatttgg aatttggaat
     6181 ttcgaatttc ggatttagta gatatccaac aaattcgcaa ttcgaaactc caaattccaa
     6241 atccgtccta gcggttgagg cgtgggtcga gcgcgtcgcg caggccgtcg cccagcaggt
     6301 tgaaggccag cacggtgatc atgatcgcca ggccggggaa tatcagcagg tggggggcgt
     6361 tgaacacgct gttgcgctcc acgcccagca tggtgcccca ctccgggtga ggcggctgct
     6421 gcccaagccc caggaagccg agcgccgccg catcgaggat gcccgaggcg atgccgagcg
     6481 tgccctgcac gatgagcggg gttagggcgt tgggcaggat ggtgcggaac agggtgcggc
     6541 ccgccggcac gcccaacgct ttgtctgccg tcacgtagtc aagctcccgg atggagagca
     6601 cgctggcgcg cacgatgcgg gcgtatactg gtatggccac gaatccaata gcgaacagcg
     6661 cgggaaggag cggcgataat ccctgcacga tgagggggcg caacacggcc acaagcgcga
     6721 ttgccagcag caagcttggg aaggccagca gcacgtccat cagccgcatg agtatattat
     6781 ccagccagcc gcctatataa cccgacagcg cccctatcag cgtgcctacg atgatggcga
     6841 aggtcaccgt ggctaccccg atgaagaggg agaccctggt ggcgtgcacc acgcggctga
     6901 acaggtcgcg cccgttgccg tcaagcccga atatgtgctg cggctcgttg gaggggcagc
     6961 cgaacaggtg gatgcagggc gttatcctca cccggttgtt ggggtcggtg aacgtcagcg
     7021 ggtcgtgggt ggctatgatg ggcgcgaaga tggcgacgaa cagcaggatg ccgatgatca
     7081 ccagccccac ctggcccgaa cggttgcgca gcaggtgccg cgtggcgttg cgccacatgc
     7141 tggccgacct ggtgggcctc aatatgcccg gttctaccgc tttctgtcgc agaagcattt
     7201 tccggctcct aactcaacct gatacgcggg tctatatacg catacgatat gtccacgagg
     7261 agattcatca gcacgaagag catggccact atgatggtga agccctgcac caccgggtag
     7321 tctctggcga agatcgaatc gaccaggaac ttgcctacgc cgggcagccc gaacaccgtc
     7381 tcggtgagca cagcgccccc cagcaacgcc ccaaactgga ggccaatcac ggtgacgagg
     7441 ggcagcaggg cgttgcgcat ggcatggcgg aaaagcacga atcgctctcc cagccccttg
     7501 gaacgcgcgg tgcggatgta gtcggccccc agcacgtcga gcaggttgga gcgcgtaagg
     7561 cgggcgatga tcgccatcgg gatggtgccc agggcgaccg agggcaggat aaggtgctcc
     7621 aacgcatcgc ccaggacgtt ccacttgccc tgcaaggtgg cgttgagcgt gaacatgttg
     7681 gatacgaaca ggaagatgcc cctggcgacc ccctcaagct ctcccatgtt ccacgccttg
     7741 tcgaggggcg ggagggccag ccccggcgtg aggcggctgc cggtgggcag ttggaacggc
     7801 gtgtcgcgca gtacgacccc gaaaaagccc gccagcagca ggcccagcca gaagatcggc
     7861 atcgacactc cgaggttcgc tatgatcatc gccccggcgt ccaacgcact cttgtgcctg
     7921 taggccgata tgatacccag cggcacgcct accgctatcg cgaacagcat cgagcctatc
     7981 gcaagctcca acgtcatggg caggcgttcc agcaggatgt cgcccaccgg gcggccaaac
     8041 ttgatggagt tgcccaggtc ggcagtcgcc aggccggtag tatagcgccc gaactgcacc
     8101 aggatgttgt cgttcagccc aaaccgcacg cgaaagtccg cacatgcggc tgccgaggcc
     8161 cgctcgccca gcatggcgat gcacgggtcg cccggaatcg agcgcaccag tataaacgtt
     8221 acgaatatga tacctagaat aacgggtaca atcagcagca aacgctgaag taagaagcgt
     8281 gccatatctt atccccaact cttacatact cttgtagcaa aggcccctgg aaggccgcaa
     8341 taagggagag gaaaaggtaa ccggggagca ggagtaccac caacccacgg caaggggcac
     8401 ccccgcaacc ctgagaatga catacagcga atgggcagga cggcttgcgt cccacccatt
     8461 cgcttacctg cctggcgacc tgggtcgcac ggcaccccga actttactcc gcgggcggag
     8521 gcgggttcag gaacgcggtg aacaggtaag tccagtagtc gaagttgcct gcacggccct
     8581 tgtggagcgg gtgcttggca tcccactgta cggcgtacgt agccaccacg tcgcgggcgg
     8641 tcacagcggt gccgtccgag aatttggcgt tcggccggag cttgatggtc cactcggtca
     8701 ggtcggtgtt cgactcgtac gaagcggcca ggccaagctc gacttcggtg ctgccggtcg
     8761 cgtagccgag cagcggctcg aagatctgct cgcaagcgcg cagggcttcg ccgtccgtct
     8821 catcggcgca gtagagcgaa ggcggctcgc cgttctggat ccagacgaag gtgtccctgc
     8881 cgggggcgga catcaccctg aacgactcgt tgccgagcgg gctggagtga gcgcctacca
     8941 cgtcagccct gtagaccaca gccgaaccgc cgtgggctat aggtatcata ggcacgtgct
     9001 gcttgatggc ggtgttggcg tcgccgtaga gggcgttgcg ctgctcctgg tccgacagag
     9061 aagcggcctg ggccagcttg gaagtaacgt cgggccactt ggtgccgaac gagtcgttcg
     9121 cgcccgcgcc gaagtgggag tcgaggaagt tggtggcgtc cggatagtcc gcgccccagc
     9181 cgaggaggaa catctccagg ttgccggcgg aagcgttgtc gaggaaggta gcggactctt
     9241 gctggtcaat cgtggcggtg atgccaagcg gagccagctg ggcctgcagt tcctcagcta
     9301 ccttgtcggg agagggcaga tagccacgca ccacgttgcg gtaggcgagc ctgatgttga
     9361 aaccgttggg gaaaccggcc tcggcaagaa gggccttggc ctggtctacg ttgtactcat
     9421 accagggatc gccctcgcag ccgccaggga ttgcgcacgg ggtgaagtac tgcgccacag
     9481 ccgatcccgg aggatagaac ttgtcaacga tagcctggcg gttgagggcc atagcaatgg
     9541 cctggcgcac cttctcgttg tccagcggcg gcttggtgtt gttcatgccg acgtagaaga
     9601 cgttgagggc cacgcggggg aagagcttga ggttggcgtc accctcgatg gtggcgaagt
     9661 cgttcgggtc ggggttgtcg atgccgtcgg cttcaccggc gcgcaggctg ctgaggcggg
     9721 cggtagccga ctcgttccag cggaagatga gggtcttgaa cgcgggggcc tctccgtcgt
     9781 agttgggatt ggggaccatg gtcagatggt cgccgcgcac ccactcctgc accatgtagg
     9841 ggccggagcc gagaggttcg tcgagcggcg cgccgccggt ctcctcaagg tgggcagccg
     9901 actggatgcc gagggagcta aaggcgatct tagaggggaa cgccgggtcc gaagcgcaca
     9961 atgtgaactt gacggtggtc tcgtccacgg cctctatcga cttcatgatg ccgccgtagc
    10021 tgcaatcgga gaccgcatag aggtcgtcgg tggctgtgat gctggcgctt gagccggtgg
    10081 gggatgccac ggcttcttcg gttggagtct cttcaggggc ggcaacagta tcggtgggcg
    10141 ccgcggccgt ggtattggtg gcctgggcag cgggcggcgc ggtggcggtc gcctgctgaa
    10201 cggttgtggg cgtaggggct gcttccggcc cgcacgctgc gagcatgggc agcagtatga
    10261 gactagccgc caataacccg gcagatttct tcattgagaa cattcttgct ttcctcctat
    10321 ggtttctcga tactcatgcg agtaccaggt acggaatgct cgaacaatta cgccaagcga
    10381 gcactcaata cgacaatacg agatgggcta taaacacgca tcaccccctt cgcgggatat
    10441 gacaatccac catgcaagta atttgcatta taagttataa caccaacatg tcaagtccag
    10501 ggttcagatg gagcagcaat agagaggcga aggcgatgca agagagccga aacaaccgtg
    10561 attcacaccc cgcacaccgc cccgatgagg tgccccggtg ctgctagtgg ccctgaaggg
    10621 tatgcctggc agcggcaaga gcacgctggg tagaggcata agccggcggc tgggctggcc
    10681 catcatcgac aaggacgacg tgaaagacct catcgacggg cactgcgacg actcggccac
    10741 actggcctac tccgtcatgt tcaacatcgc ccgccgccag cttcagcagg gcctcaacgt
    10801 catctgcgac agccccctca cctacgctgg cctgtacgcc caggcacggc aagtagcaag
    10861 cgaggccggg gcgaagctgg tggtattgga atgcatgtgc tcggacgaag cggagtggcg
    10921 gaggcgggtg gatgctagag gggagttggg actgccctca caccacatga gcagttggga
    10981 caagttgcag gcgtaccggg ggatggttga ggggaaggta gactacccgg tcgcagacga
    11041 gcgcctggtg ctggatacgt gtggggcggt tgaggagctt gtggaggagg cggtgggatg
    11101 gctgagggag cactgaggga gcaatgatta ttcgtgtacg catagggtat ttacagatct
    11161 gaatttccgt tacgtgaatt aagccgattt tcctcagtgg ccaaattaat aatatgggcg
    11221 acaaactgtg tgcgagcata gaaagcacgt gatgtgctcc cgtgtcc
//
LOCUS       DASRWZ010000834         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U11719
            k141_274047, whole genome shotgun sequence.
ACCESSION   DASRWZ010000834 DASRWZ010000000
VERSION     DASRWZ010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970245
            Sequence Read Archive: SRR6517650
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,659
            CDSs (total)                      :: 2,642
            Genes (coding)                    :: 2,617
            CDSs (with protein)               :: 2,617
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 0 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 7 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274047"
                     /isolate="SMAG_U11719"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.45 N 104.96 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3038
                     /locus_tag="VFH60_13295"
     CDS             <1..3038
                     /locus_tag="VFH60_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6314805.1"
                     /translation="PRRVLPLQWSAWLAKPGVEPLLVTVFFIELTVLMTWPWTLHMGQ
                     AINPFGDVIVQMTTLQWNAHALATNPGGLFEAPFFYPYRHSLAFSENLLGETLLTLPI
                     LLLTGNPALASNFYILLTFVLTGLFTYLLVRDLTGSRLAGVLSGVAFAFCPFRFMQMG
                     HLHMLSTQWFPFTLWALRRGLGVLDYGHWTSRYGAIQQKSIVQHPRATLWLILAALGF
                     VMMGLSSVYYTYFLAIAVLIYVIWWGIVEFREAVKVADVKWAPVGAGVFIALLVVVLI
                     LGPVFWPYLQLNQDLGFARTTYEVQNWGANWIYYRNVLASNWLYGQVLAPSWVSTLGE
                     RELFPGIVASILALGGLVLGRGRERWFYPILGLIAFVLTFGLSHNLPGTSREVPLPYA
                     LLYDWVPGFKALRVPVRFAVMLDFAIYVLAGYGLARILQLLRRTEKVEQTQTPSVLED
                     NLGSIVLGTQRLQPIEPEVVEVVVKPNRRTTVAVAGVLTLLVLVEFINPLDTANKRDV
                     AAQLETIEPYGWLSRPENAGPVLELPMSADQPDVWYTFFGTKHWQPLVNGWSSFVPPG
                     TMELKLALDAFPDARTVSMLQGLEVRHVVVHLWQYPQDQQAGLKQRLDADPRLKMVNQ
                     AGDNYVYEITPDPWLRKIAEDVLARDAYIWVGEAKSGSMPGFEVLAYALNLFGVPQGH
                     IGGNINIGHRHIGSLPFGVPPDYVLTPNLDGDDDTPFGFEYMQELPTTESVRFLQSPP
                     GIIKIYDLTQRNAPNTDLDNLRMGVSTAGINFGDSPNRDAAPGGVLLNMTFLSFAPAR
                     IDVQLGSGRVVSVTLPVGVSKVSTRGFPSPREVKLEQRSGDATLLKVDLLEAAGAPPS
                     GPDAFLLPPADLVPVPIEVTTSRDGDFLDMRMRVVTPQGGDEFAATLDVYSEPWGTHP
                     EGHFGYWSVVIPADGVAHDYNLRLDPILKTVTATRDATPVEAFAWQGPPNQGDFRATL
                     HITRKGSLVATVPLYLFTLAEGRLASWYAEPSHLTIVRP"
BASE COUNT          483 a         1053 c          967 g          579 t
ORIGIN      
        1 ccccgcgccg ggtgcttcct ttgcagtggt cggcctggct ggcgaagccg ggcgtggagc
       61 cgctgctcgt cacggtgttc ttcatcgagc tcacggtgct gatgacctgg ccctggacgc
      121 tgcacatggg gcaggctatc aacccgttcg gggacgtgat cgtgcagatg accaccttgc
      181 agtggaacgc ccacgccctc gccaccaacc ccggcggcct ctttgaagcg cctttcttct
      241 acccctaccg ccattccctc gccttctccg agaacctgct cggtgagacc ctgcttaccc
      301 tgcccatcct gctgctcacc ggcaacccgg ccctcgcctc caacttctac atcctgctca
      361 ctttcgtgct caccggcctc ttcacctatt tgctggtgcg cgacctcacg ggaagcaggc
      421 tggccggggt gctttcgggc gtggccttcg ccttctgccc cttccgcttc atgcagatgg
      481 gccacctgca catgctctct acccagtggt tccccttcac gctgtgggcg ctgcgccggg
      541 gcctgggcgt gctcgactac gggcactgga cctcgcgcta cggggctata cagcagaagt
      601 cgatagtgca gcaccccagg gccacgctct ggctaatcct ggcggccctc ggcttcgtga
      661 tgatgggcct ctcgagcgtc tattacacct acttcctggc aatcgcggtg ctcatttacg
      721 tcatctggtg gggcatcgtg gagtttcgtg aggccgtcaa ggtggccgac gtgaagtggg
      781 cgccggtagg ggcgggcgtt ttcatcgcct tgctcgtggt cgtgctgatc ctaggcccgg
      841 tcttctggcc ctacctgcaa ctcaaccagg acctcggctt cgcgcgcacc acctacgagg
      901 tgcagaactg gggggccaac tggatctact accgcaacgt gctggcgagc aactggctct
      961 acggtcaggt gctggccccc tcctgggtct ccacgctggg cgagcgcgag ctgttccccg
     1021 gcatcgtagc ctcgatactg gcgctggggg gcctcgtcct ggggcggggg cgcgagcgct
     1081 ggttctaccc catcctgggc ctaatcgcgt tcgtcctcac cttcggcctg agccacaacc
     1141 tgcccggcac gtccagggag gtgcccctgc cctacgccct gctgtacgac tgggtgcccg
     1201 gcttcaaggc gttgcgggtg ccggtgcgct tcgccgtcat gctggacttt gcaatatacg
     1261 tgctggcggg ctacggcctc gcccgcatat tgcaactgct gcgtcgcacc gagaaggtcg
     1321 agcagaccca gaccccctcg gtgctcgagg acaacctcgg ctccatcgtg cttggcacgc
     1381 agaggctaca gccgatagag ccggaggtgg tggaggtggt ggtcaagccc aaccgccgca
     1441 cgacggtggc cgtggcgggg gtgctcaccc tgctcgtgct ggtggagttc atcaacccgc
     1501 tcgacacggc caacaagcgg gacgtggccg cgcaacttga gacgattgag ccttacgggt
     1561 ggctgtcgcg ccccgagaac gccgggcctg tgctagagct gcccatgagc gccgaccagc
     1621 cggacgtctg gtacaccttc ttcggcacca agcactggca gcccctggtg aacggttgga
     1681 gcagcttcgt gccccccggc actatggagc ttaagctggc cctcgacgcc ttccccgacg
     1741 cgcgcacggt gtcgatgttg caggggcttg aggtacggca cgtggtggtg cacctgtggc
     1801 agtaccccca ggaccaacag gcaggcttga agcagcggct cgacgcggac ccacgcctca
     1861 agatggtcaa ccaggcgggc gacaactacg tgtacgaaat cacgcccgac ccgtggctgc
     1921 gcaagatcgc cgaggacgtg ctggcaaggg acgcctacat ctgggtgggc gaggcgaaga
     1981 gcggcagcat gcccggcttc gaggtgctgg cttacgcgct caacctgttc ggcgtgcccc
     2041 agggccacat cggcggcaac atcaatatcg ggcacaggca tataggctcc ctgccgttcg
     2101 gcgtgccgcc cgattacgtg ctgaccccca acctggacgg cgatgatgat acgcccttcg
     2161 gcttcgagta catgcaggag cttcccacga ccgagtcggt gcgcttcctg caaagcccgc
     2221 ccggcatcat caagatatac gacctgaccc agcgcaacgc ccccaatacg gacctggaca
     2281 acctgcgcat gggcgtgagc acggcgggga tcaacttcgg tgacagcccc aacagggacg
     2341 cagcgccagg gggcgtgttg ctgaacatga ccttcctctc gttcgcgccc gcccgtatcg
     2401 acgtgcagtt gggtagcggt cgcgtggtga gcgtgacgct gccggtgggt gtatcaaagg
     2461 tgtctaccag gggtttcccc tcccccaggg aggtcaagct tgagcagcgg tcgggcgacg
     2521 ccacgctcct caaggtggac ctgttggagg cggcgggagc gccccccagt gggccggatg
     2581 ccttcctgct cccgcccgcc gacctggtgc ccgtgcctat agaggtcact acttcgcggg
     2641 atggcgattt cctggacatg cggatgcgcg ttgtcacccc gcaggggggc gacgagttcg
     2701 cggccacgct ggacgtatat tcggagccgt ggggcacgca cccggagggg cacttcggct
     2761 actggtccgt ggtgataccc gctgatggcg tggcccacga ctacaacctg cgcctggacc
     2821 ctatcctcaa gacggtgacg gccacccgcg acgccactcc tgtggaggcg ttcgcctggc
     2881 aaggcccccc caaccagggc gactttcgcg ccaccctcca tatcacccgc aaaggcagtc
     2941 tcgtcgccac cgtgcccctc tacctgttca ccctcgccga gggccgcctt gcctcctggt
     3001 acgccgaacc ctcgcacctg actatcgtgc ggccgtaacg tttgtccagc gtggcgaaag
     3061 agcaagctgg agccttctga tt
//
